LOCUS       DAOQGF010000001       164351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13_length_164351_cov_7.871762, whole genome shotgun sequence.
ACCESSION   DAOQGF010000001 DAOQGF010000000
VERSION     DAOQGF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 164351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 164351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..164351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_164351_cov_7.871762"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1755)
                     /locus_tag="PK937_00005"
     CDS             complement(85..1755)
                     /locus_tag="PK937_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH30985.1"
                     /translation="MANSLFRTKSIDKIMREAEEGMSDAHTPHLSKTLGVRDLTALGV
                     AAVIGAGIFSTIGAAAYDGGPGVIFLFLITAVTCGFTALCYAEFASRVPVAGSAYTYS
                     YVTFGEVIAWIIGWALILEYAIGNIVVAISWSSYFNNLLEGLGLHLPVWLTVDYQTAR
                     NIYQDALRNNLPTDQLVWTMAPSFAGLKVVMNLPACIIVILITILAYVGIKESKNSAN
                     LMVALKLLVLFVVIAVGVFYINTDNWTPFMPNDFTGVLKGVSAVFFAYIGFDAISTTA
                     EECSNPKRDLPRGMMYSLIICTIIYVITTLVITGMVNYKEFQGVSDPLAYVFEKIQME
                     KLGYIISFSAVVAATSVLLVFQIGQPRIWMSMSRDGLLPKKFAEIHPKYHTPGFATII
                     TGVIVAIPALFIEGGIVTDLTSIGTLFAFVLVCGGVLLLPAKKEQEDNRFHLPYINGK
                     WIVPFLLLVFIYFFSERIGSAMTHIGNEAYQEVLFLIFMVIAVVMAVLTFVRNYSLIP
                     ILGMMCCLYLMIEIPVKSWFVFFIWMAVGLSVYFMYGYKNSHLSESKS"
     gene            2003..3334
                     /locus_tag="PK937_00010"
     CDS             2003..3334
                     /locus_tag="PK937_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH30986.1"
                     /translation="MSETILTLIISVSLFALVFIPYLWYTNKKRVRFEAKKREAITLG
                     HDKPVAQHPLIDQSRCIGCAACVIACPEHALGMIDGLAELVYPAKCVGHGVCAEACPV
                     SGIRIVLDPTKSTTELPLLDESFQSNIPNLFLIGELGGMALIRNAIFQGRSVIEYITE
                     NIRNTNSSPASSDIHDVIIVGAGPAGLSAGLTAKKNGLDYVLLEKETQAGGAILSYPR
                     QKLVMTTPVEIPLYGLLKQRELSKENLLSMWNDIIQKTDLAVCCDEKIVNVSCENKIF
                     TVYTSAGHTYRSHHVVLALGRRGTPKKLQIPGEDLPKVAYQLLETVRYKHSRSLVVGA
                     GDSAIEAALALSKQTGAEVTLINRGGEFYRAKPKNQDRIRDAERNQKIKIYYQSHVLQ
                     IDPTSVLVQTSNGQVSVENDFVFIFAGGEMPTPFLKQIGIEFIQKSKVIAA"
     gene            3331..4209
                     /locus_tag="PK937_00015"
     CDS             3331..4209
                     /locus_tag="PK937_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH30987.1"
                     /translation="MNKRMHRWYILSMTVAGFIVVLSIGIYGWDYYGTPLPLRHDHVQ
                     HGLLSPTGFWGHGMGFIGAFLMTFGVLLYSLRKRARWMAAVGQIKHILELHIFLCLLG
                     PALIVFHSAFKFGGIIGVSFWCMVIVVASGVIGRYLYLQIPKTITGREITPIELEKQI
                     TTLRDRLQKDDGIEEYLLQRLDALSAAFTETSRLSIIRAFPAMIIQNLRHDARVRTLL
                     QEFEKNRNPFSSDVHTRLTMKAHLQRQLTNLTLTQKMFRYWHMFHLPFAVVMFIIMIA
                     HIVTAFLFGYGWIFTS"
     gene            4226..4801
                     /locus_tag="PK937_00020"
     CDS             4226..4801
                     /locus_tag="PK937_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH30988.1"
                     /translation="MKKHLPVLLIAILLLAIAAYQTLETQQTTSVKTSSNKPRPTLGL
                     MKDQNPDNNSQVFAIKQLQDYLQGHPDDADHWIQLGNLLFDNGNFADAVKPYTEALRL
                     QPENPDVRTDLGVCLFNTGMNDEAILQFERALQYAPDHITATFNLGIVHTRMGHTPSA
                     VQYWRKTIRLGPDTDMGKKATRALTDAGYKP"
     gene            4804..6567
                     /locus_tag="PK937_00025"
     CDS             4804..6567
                     /locus_tag="PK937_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH30989.1"
                     /translation="MKHFLHTMLSVMMAWSLHAQFSPGDLASSHKQLEGIQQCTQCHD
                     LGKKIDDARCLDCHTLIKTRIANNKGFHSSAETKNKACIECHHDHLGRDYAMIQWKPS
                     REKFDHTQTGYILDGKHQTTQCEVCHKEAFIKDNAVRARTGDGLLLNKTFLGLSQDCR
                     SCHFDEHRDQFDQCLNCHDFNDWKKSSEKIFDHNKSKYPLTGLHAKVSCEKCHTSVAD
                     PRQKADHKTDETYFKYRPLQFANCTPCHADPHQNRFGQDCQKCHLTASWKQIKNALFD
                     HRQTRYPLEGLHITVRCEKCHQPDSIKPAVYKNMSFSNCTDCHTDAHVGQLTQRSDQG
                     RCETCHNVNGFRPSRFTVSMHATESSYPLIGAHEKTDCNKCHIKANASEFLRRTGIRP
                     RVDSTHIILKFKEKTCQSCHTDIHRGQFADKLKSNDCDICHKTTTWKDIVFDHQKNSD
                     FKLIGKHEKTPCEKCHLLVDVGTEIKRTLYKPIPTYCERCHNDIHEGQFAEYKTMTPS
                     HPKTDCAKCHSNDAFKPALFDHEKQSQFPLTGAHIKVECVKCHIRARITSDTLNVVYK
                     PISTECAACHPDQHEGVFQQR"
     gene            6564..7319
                     /locus_tag="PK937_00030"
     CDS             6564..7319
                     /locus_tag="PK937_00030"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH30990.1"
                     /translation="MNRSLIIAAALLSMAAYLPQPDPEYTAWTYARPAADTPYVKIPH
                     KPFDIPCDACHTTTTWKILKPKIEYDHTKTGFPLVGAHLQTACSQCHGGGVFRSAIRE
                     CFVCHTDPHQAQLGLECERCHTEYAWAPSIFKHEDQNLFMFGAHKGLDCTGCHKNLIT
                     FAMPNVQECGDCHIPLGATTDHNAYKSIGDCMACHNMNGWSNYPHADEWFSLTGHHRR
                     SCETCHKQIPNYTTYTCRDCHRFDHKGEGHDDD"
     gene            7309..8613
                     /locus_tag="PK937_00035"
     CDS             7309..8613
                     /locus_tag="PK937_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH30991.1"
                     /translation="MMIKFLNRVIIIILISYFFIVKDGRSQSLTGNKNPFIARFSSGW
                     NYYSRGASLNPQLSEFTQYAMLNYRTLDDSNRIIWQIRTDIYQKDAVTQTRLRNQNEQ
                     FDHRTVIRQAYGSYTLRDGEVKLGRFVPIGTHTDAYPINGLGAENTRITSRWRGSVYG
                     GKIFDEYDNRPEGIGYSTGGSLTYDQTRWQAGAGLSAEQLRNTRLTKAYLFGEYRPSY
                     TWRIVSNNQYVVNKALLGYSQNTLYYRLNKNLSTRFFAEYHDRRAYFPAPTDSLSMDR
                     FFNTTQELMIGGSVRYRIFQWRKIGMLETMPSLKKRLGNDNLTYAALQFFYQNYFFWR
                     FNLNTGFSYTDNQWIRNYRSHLSLNKDFFASRLDASFSLIVNTYTWNSTSSRAKLLTT
                     VSADATYRFDASWYISAGIYEEVGNATDPHSGVNIRMFYYLH"
     gene            8699..9700
                     /locus_tag="PK937_00040"
     CDS             8699..9700
                     /locus_tag="PK937_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNH30992.1"
                     /translation="MAVADLTRKTILQGDIAQSGMTSILSLIKLSQKSGTLRLKDGEK
                     NASIRIEKNYFIEASAPEEILLGEFLVREGYLTEQDLAKTLPLQKRMHIPLGQLLQGM
                     GYFGLDANALTRILNRYLREVIFRIMKWNRGTFTVEETVKKDFPVREEIPVRLNMDFV
                     LLDTTQRLDEWKRLASSVTSLQSALKINRRAISENASLTLNADQLTVLSYVNGRRTLV
                     RILEKIGHNEMFYLSVIDDLLKRRILIERQAEAMRVIIPGRIKADAVNRNRQFPGKFA
                     ANLLYKEIDGKKNLHELADSLHFELPDLWENISLLLKNEAVEIIEGRREFHNLSEEM"
     gene            9924..12605
                     /locus_tag="PK937_00045"
     CDS             9924..12605
                     /locus_tag="PK937_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH30993.1"
                     /translation="MKNRLPRFPLYLAAVLFFVIPTFLQAQVVGKITGTIVDASNGDP
                     LIGANVFVLGTKLGGITDLDGNFIIGKIPVGTYSIEISCLSYKKKIVHNLTVNDGLAS
                     TLKISLEPEALEGEEVVVEAEVLKTQESGLLLAQKKSSKVMDGVSSEQIAKTSDGNAG
                     AALKRVTGVTIVGDKDVYVRGLGNRYSNVQLNGSVLPSTNPNQKETPLNIFSSNVIDN
                     IIVQKTYTPDQPGEFSGGSVQIETKDFPESRTIKIGVSSMFNSNSIGNDYLNYNGGGS
                     DFLGFDDGTRKLPTAAKQGRIGTSQGVTAIRQFQNSWSPRSSRTTPGQSYSISYGDQF
                     AVGSRALGFIAAINYSYQNQVRNEVLRNLQQAQSLASDYDASRGMTSTLLSGMLNVSY
                     KINPTSKVSLRNLYTNVSDNTTSRVEGYYYNSGGDYRQTQLRFSQRSIFASNLQYDTY
                     IKEWMDSKILIEATMAAANAYEPDTRNTHYAYNSTLHQYEIVLDQRTNTHFFSDQKDR
                     DYNVKLNWDLKLLPTLRLKTGGLFYDKQRDFEARRFAIAGDPQSVYPAELKTTNPEEA
                     LNPDLFSNGGLIFFETTRNTDSYRGDQNIYAGYLSADITIGNRWNLIAGARAEQSIQR
                     IDKNEVLNTADLLPAVNLTFKVNDRANLRAAFSATLARPEFRELSNFFYSDFVGARTV
                     YGNPDLKRTKIKNYDLRWETYPGIGEYFAVSGFYKHFTNPIEIFHRLSTNPEVYYGNI
                     AEAELWGVELEARKALTEALRVTANLAVMTSSVNYGKAVYSQANRNRPMYGQSPYTIN
                     LNGFYVLRSATEFSLAYNRFGKRLSSVGNVSQGEDEYEMPFDKLDFTATQKFGKWTYK
                     LSIQNLLNERVINRQGDIITRDYAPGVTYSVGASYDF"
     gene            12709..14049
                     /locus_tag="PK937_00050"
     CDS             12709..14049
                     /locus_tag="PK937_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH30994.1"
                     /translation="MKKSLILLLLIGSIQLISCGDDDKKDTPAEVPSAYTSLPGTLIV
                     EDETWSGTKTLTGAYYILPGVTLTIEPGTVIKFTYHNNNPDSVGAIFALKADPTNFST
                     PRASARLEAEGTAANPIIFTSAKTTKAAGDWGGIVLIGEAPNNIPGGIGDVEGVSSEI
                     QYGGSNAADNSGTLKYVRIEYCGFGLITDSELNGLSLYSCGSGTTLDHVQVYKCTDDG
                     FEFFGGSVNAKYLVSAYNDDDSFDHDQGWNGKGQFWLGVQTTGADNGFESDGRHTLGS
                     GNPTKPTIYNVTLYGLGTGKDAADGNRGMRLREDFEGTLKNFLITNFAGVAWRLESAS
                     GDSTSRNYDKGLLTITNAVVYNNDRDVTVAGNYGGFNSLNDSTRFFAAANVTEANPNF
                     TNVGSKDFSVQAGSPCLSNAATPPSDGFFDVSATYRGAMGSTNWATGSWVRWSD"
     gene            14226..14750
                     /locus_tag="PK937_00055"
     CDS             14226..14750
                     /locus_tag="PK937_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH30995.1"
                     /translation="MNFSLAQSIEILERTPMILTAWLDGLSPEWTSSNEGPDTWSAYD
                     IVGHLIHGEKTDWITRAEIILSDLPDKTFKPFDRFAQFEDSKDKTLRQLLTAFTELRA
                     KNIKKLQSLNISEQDLSRTGLHPAFGTVTLVQLLSTWAVHDLDHISQIARVLAKRYKD
                     DTGPWVAYLKILRQ"
     gene            14819..15238
                     /locus_tag="PK937_00060"
     CDS             14819..15238
                     /locus_tag="PK937_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH30996.1"
                     /translation="MNVIIRNVFAVIVGAIVGSIVNMSIIMIGGQLIPPPAGADVTTM
                     EGLKSSMHLFELRHFLMPFLAHALGTLGGATVAALIAANHKMKFALSIGFVFLLGGLI
                     NVFMLPSPVWFTVVDLAGAYLPMAYLGGKWMSKNQNI"
     gene            15498..17360
                     /gene="htpG"
                     /locus_tag="PK937_00065"
     CDS             15498..17360
                     /gene="htpG"
                     /locus_tag="PK937_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNH30997.1"
                     /translation="MSSQTLEFKTEVKELLHLMIHSLYSHKEIFLRELISNASDAIDK
                     VRYLSLTDSQISGGDADWKIKLKIDKEAGTLTISDNGIGMTEEEIIRSLGTIAHSGTR
                     DFIQRLTEKQVKDNPELIGQFGVGFYSSFMVADRVVVISRKAGEPQDKAIRWESKADG
                     VFTVESFEKATRGTDVILFMNEEGKKYLEEREIRSVVRKYSDYIEHPICMDVEKDKKI
                     EEETLNSRKAIWLKDKSEITTEEYNDFYKHVSHDFGEPLKTIHFRAEGTSEFSALLFI
                     PKKAPFDLFYKEFKIGPMLYVKRVQIMDHCEQAIPTYLRFVKGVVESSDLPLNVSREI
                     LQANKQMEVIKKNIAKKILDALSDMKTHEFEKYVEFYNEFNRALKEGLYSDHQRKESL
                     RDLMLFESTRTEKGKYTSLKTYVDGMKSDQEFIYYMTGASREELERSPYLEAVKDKDY
                     EVLFLTDEIDEFIVSELEYSGKKLKSLTKGDIELDKDKKESKKEAEKKYGKLIDLLKD
                     QLKEEIKDVRVSARLKDSACCLVADEDGMDQNMERIMKALGQNVPSGKRTLEINPDHP
                     LIGKMQMMFDIDKDQPLLKDYTALLYDQALVLEGSKPKDPVLFAKRIAEIMVKA"
     gene            17588..18952
                     /locus_tag="PK937_00070"
     CDS             17588..18952
                     /locus_tag="PK937_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNH30998.1"
                     /translation="MISGKDLIAMGYKPGKWFKDAIEHINENHLTDEKLENYLQQISP
                     KEVLPLDKAIPYFSNIRAENEVELENIKSVDTTMMELMKTPTMIKGAIMPDACPTGEK
                     GQIPVGGVAVAQNAIHPAMHSADICCSVMMTGFGKIAPKSVLDAAHAITHFGPGGRDE
                     FSNLPSELVEQICSNPFLNNERSKTLAATHLGTQGDGNHFLFVGVSAKSGETIMVTHH
                     GSRGFGAQLYAQGMKMAEFFRKEISPKTRACNAWIPFDTGEGNNYWEALQIVRVWTKL
                     NHRVIHDAVLHSLHADITERFWNEHNFVFREKDLFYHAKGATPLDDKFVPDSTDGLRL
                     VPLNMSQPVLVVKGATTKNNLGFAPHGAGRNISRGQHKRLYAHKTKEQLFAEETRGLD
                     IRFFSRHIDVSELPSAYKNADAVKAQMKEFGLGEILDEIIPYGCIMAGDWEKNAPWRK
                     KKNK"
     gene            19423..>21503
                     /locus_tag="PK937_00075"
     CDS             19423..>21503
                     /locus_tag="PK937_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH30999.1"
                     /translation="MKKVVFYWLVTVLCLTSFAAAQYDSSLFSGMKARSIGPAGMSGR
                     IASIDAVKSDPNIIYVGAATGGVWKSINGGITWNSIFDDQSTGSIGSVAIFQKNPNIV
                     WVGTGESNVRNSVGVGRGIFKSIDAGKTWQFFGLEKAEKIARVILHPENADIAWVAAM
                     GTTWGENKERGVFKTTDGGKTWKQVLYVDEKTGAAELVIDPSNPNKLLCGMWEHRRYP
                     WYFKSGGTSSGLYISTDGGETWKKQTTKEGLPEGELGRIALSFSRNKTSTVYALVEAS
                     ESVLLRSDDGGYKWRTVNKEKNVHPRPFYFSSIEVNPVNENIVYRIHVTFDVSEDGGK
                     SFSLLTPYQNIHPDFHALWMHPNGEMMIAGNDGGLAFTYDRGKTWRFVQNLPVGQFYH
                     ISYDMEYPYNIYGGLQDNGSWRGPSTTFKRDAIYNDVWDMVAFGDGFDTEPDPENADF
                     GYAMSQGGALVYFNHKTGLQKFIRPTESAVKHRYNWNAGFALDPFDAKTIYYGSQFLH
                     KSTDKGDTWEIISPDLTTNDTTKINLGKETGGLTLDVSGAENHCTILTIAPSSVKRGV
                     IWVGTDDGNVQVTQDGGKTWTLVSKNKSIAAPAGTWVPHIEASKFDAGTAYVVFDDHR
                     RSNWSTYVYGTRDFGKTWTNLATKEVDGFVHCFEEDPVNKDLLWLGTEYGLYVSFNSG
                     KNWMKWRAGVPT"
     assembly_gap    21504..21603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21604..22709
                     /locus_tag="PK937_00080"
     CDS             <21604..22709
                     /locus_tag="PK937_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31000.1"
                     /translation="VSFNSGKNWMKWRAGVPTVPVYDLATHPREHDLIIGTHGRGIYL
                     LDDITPLRKFAENSKKNAHLYEVNPGYQYNYSFWSGSNAGGPGNAAFKGEQRPYGSII
                     TYFVNTSDSIKALEDTPKEPKMKIEILDKDSVIRVIKGGMKRGMNRVAWDLSRGAFKG
                     ISSVDSTDLEASGIYVLPGMYTVRMRYNGQEYKQSVEVKNDPRFAISTDGRKAMQTWA
                     EKAGELQTAAATVYQQIAESKKSITAMLAVTGNLDSAKSNPIKRLAESAQKKMSGIQD
                     KIQSELSKQGFNDNSDELLQQIGELQFYITSHYEAPTQQAKVKYERLRKVLEGLIEDH
                     KKIQNTDIPELQKLISNAGVNYFKQPQEIKLNK"
     gene            22811..>23684
                     /gene="aroB"
                     /locus_tag="PK937_00085"
     CDS             22811..>23684
                     /gene="aroB"
                     /locus_tag="PK937_00085"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNH31001.1"
                     /translation="MKTKAAPTKKLTVPLGQRSYPITISDKISTSIVPQKKSVEKTIV
                     ITNETLAFLYQKKLSRFFSARWIVIPDGESHKTLSTLEHIVTQLIDFRADRQTQILAF
                     GGGVIGDIAGLAAATFMRGIPYVQVPTSLLAMVDSAVGGKVAVNHPLGKNMIGAFYQP
                     RSVHIATDFLKTLPDREMRCGLAEIIKYGIALDAKFFDWLENNQDLVLTRQPEAVSFC
                     IHRACSIKAAVVASDEKDTGRRMILNFGHTFAHAIERVSGYEPIKHGESVFLGMVAAL
                     CISLRLKKLSGANFD"
     assembly_gap    23685..23784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23785..24093
                     /locus_tag="PK937_00090"
     CDS             <23785..24093
                     /locus_tag="PK937_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31002.1"
                     /translation="AALCISLRLKKLSGANFDRMMAVLLPQVDDIFSRSEVKKSWLAL
                     DKKDVLNALYHDKKVSQNQIQWIMSTAIGKTEIVSGIHKKHTEEAFDFLNYIIHETLA
                     "
     gene            24077..24715
                     /locus_tag="PK937_00095"
     CDS             24077..24715
                     /locus_tag="PK937_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNH31003.1"
                     /translation="MKPLHKIPLFPLGVVLFPGMPLPLHIFEERYKAMIAECLEQQSV
                     FGVVYYTGENFYKIGCTARIRDVLKRFDDGRMNIVTEGCDRFSIHRVTNDKPYLQGEV
                     EYIDDISENIVEETELSRAAAQTFKDAVKLINKEDVPDLNLLDAKRISYIIASSAGFS
                     LNERQAFLEMNSTAERLRKATSALRRLMERHKMTSDIESIISGNGYLHNSKL"
     gene            24733..26340
                     /gene="murJ"
                     /locus_tag="PK937_00100"
     CDS             24733..26340
                     /gene="murJ"
                     /locus_tag="PK937_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNH31004.1"
                     /translation="MSDLHNNGKKIFRSQQAGIAALFVTVATLLSTVLGLVRDKVMAH
                     YFGASLDTDLYNYGTRIPDALQTILIMGVTSSSFIPMFSEFLAQRGHEAANRLASSFL
                     NITLTVFIGICLLIGIGMPVICDVWLSPDLPLDQKNTIVHIARIFLASQIAFALSKIL
                     SGILQTHKHFTAYGLALLVYNPAIILGMVLFHDTMGIDSAAYGALLGSVFVVLVTLLD
                     LRGTGYQYNFQWFFDKEGVRRIFVLAIPNFLNMALLQFVILAYSKISIHMPDGSFSAF
                     NYALNFESFPVSVFGISFVTAIFPYLSENASQRNFQNFNYNIQNSLRQILFLTLPSGI
                     GMAILSYPIISLILGGGRFGSEAIEMTASTLFFYALVVPLESLWYLFARAFYAIKDTW
                     SPFWYRFIGTVINLGISYSLAQSMGPSAFSLGLLAAFLIQIVLFTFGLKRRVPEVDIR
                     SAAVTGGKLFVCALAQGAVVWLLVQWLPTLSFIHQKAQRMQDLMVVGISIFAGMIIYF
                     TLTAVFKCADFSVLQRVLNRIMKKESV"
     gene            26341..27717
                     /locus_tag="PK937_00105"
     CDS             26341..27717
                     /locus_tag="PK937_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH31005.1"
                     /translation="MMSHVLIVDDEKNIRRTVEMILQDTYTVSTAESGREAFNILKEQ
                     PIDLVFLDLLMPEMNGIDVLKEIRTINPDTTVVMMSGHGTIENAVEAVKLGAYDFIEK
                     PLTKEKLIIAAKNALERVSLKEENRILRQEVNKRFEMVGESPGMKEIKATISKISPTN
                     ARVLILGESGTGKELVARAIHDNSPRKSKPFIKVNCAAIPEELIESELFGAVKGAFTG
                     AVADRDGKFVLADKGSIFLDEIGDMSLKVQAKVLRALQEGEFEKVGGTKTIKVDVRVI
                     AATNKDLKEEVKKGMFREDLYFRLNVVPILMPPLRQRKADVPFLIQHFVKLCAAEGGL
                     PEKTFSDEAMQTLSDYGWPGNIRELRNVVERTLILSLTDIVQREDLPRQILAAESTTA
                     VATNLNYEDGKTLKDFKEEAEKQYIIQVLQKNNWNVSRTAEELDVERTNLHKKLKQYN
                     IEMDKSEN"
     gene            27894..29150
                     /locus_tag="PK937_00110"
     CDS             27894..29150
                     /locus_tag="PK937_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNH31006.1"
                     /translation="MKKMFLLIAGSMLLWAGCTSAPRMRSIPEGAVIPEVKVLIARSS
                     QSVNISSKEAFAVKTDDNQDVGFISAGQNVELRFNENRVEIYDATGKSLGAYGNLIFT
                     TKSSSQRYIINNKQYRGSLWVTTQEGKILVINRLDMESYLMGVVRNEIGNLGMDQIEA
                     SKAQAVAARTYAIKNKGKYKIFDFQSDINDQVYDGAGTETEVSNQAVIETLGEVAEYD
                     SKPIDAVFYSTAGGVTTRSHWVWKSSDPKPYLNSISTKIGDKALDEASPHYRWEVKWT
                     GEEIEKIIKTNLPTVLAVVYPNEDFSKLATQRLYNLAVVSRDSSQRVQEFKIGFSKDS
                     YTVTGEQARRIMRGEKYMLYSSLFRLDVMRNADGTIQAVNCKGAGFGHGVGMCQYSAR
                     EMAKTGYSYKQILKFFYRGTDIRKMY"
     gene            complement(30076..30510)
                     /locus_tag="PK937_00115"
     CDS             complement(30076..30510)
                     /locus_tag="PK937_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31007.1"
                     /translation="MGITSIKYLKKIILLFLISSVACKDGGERVFFRSKDGKYTATMI
                     KTSHRSYYITLCEYEADTIPDLFIMPKFNKLDVMNPVMVFFISWNGDTCVVNTYANDV
                     EFSIKRSPLLIYKEYCTPTSNDYVEFHRLKKLNSENYIYIDE"
     gene            complement(30855..31616)
                     /locus_tag="PK937_00120"
     CDS             complement(30855..31616)
                     /locus_tag="PK937_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31008.1"
                     /translation="MCKESLLLIILILIGCSKTPKQNVPTAFNDSLLAATSIEDSIQD
                     VADTLTRFDGLLPAFFVPVGKYGYGLPNLIGGKRVLPSYKNYLSMSDTIDLSKIRYIL
                     KQDSVLIIVADISGLRTGLILALKRTSKDYLLLSAEEIGSVKTWSLSNSINSSMLFEV
                     TSYPYSIMCEMMQGYHVFKLDNRFGLIRVFDGVLFMTYSEKSELCPENTSYVQEFELS
                     QSGNKILLRALKLDEKGLKTEKRYVLGTDKFYPIQ"
     gene            complement(31618..32829)
                     /locus_tag="PK937_00125"
     CDS             complement(31618..32829)
                     /locus_tag="PK937_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH31009.1"
                     /translation="MTLDKNKGITSIEYNYLNLPTKVEFGSSSNRIEWKYAVTGAKLR
                     KTVYTNGTVTSTIDYVSGFVYTSNNLNYTLDYFFTETGRIKRTGTGDLQYEYDIKDHL
                     GNTRISFADLLPTPNQQPDILMESHYYAFGMRIEGLGSKSDNKYLYNGKELTEDFGLN
                     WYEYGWRMYDPQLGRWHAVDPADEYNTPYVFVGNMPTIKIDPDGSFDIFYFEIDGSVT
                     VVPAPGPPRYFVRVPVFFEIAGQKWGDYMWTEYFINDDLLAFARGIYAEFGGRPQIEK
                     ETAAEIIDNRKESETYKHTGNTYKGVLEDRKDGVQFSSTIKGNGRYDHYTNPYKITND
                     DPREFQQFLASVSAAVKIAFGNYNRTNGSLFFYSPLRMEPPFSVPGWVNGKTETTPPG
                     VDPIYMRTYKK"
     gene            complement(32923..33102)
                     /locus_tag="PK937_00130"
     CDS             complement(32923..33102)
                     /locus_tag="PK937_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31010.1"
                     /translation="MKNFDCVEMKHKAQQIIHQRLEGMSADEKMKYWQEREEDLRKRI
                     ELAKAKKSEEIIISR"
     gene            complement(33089..33553)
                     /locus_tag="PK937_00135"
     CDS             complement(33089..33553)
                     /locus_tag="PK937_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNH31011.1"
                     /translation="MKNNRVYVDTSVFGGMFDPEFERASKTFFEQIKEQRFSLVISPT
                     VADEISLAPENVKMFFEEMLAYASMLTLNPEATQLQKAYLNAGILSKKSEEDALHVAL
                     ASVSECSMIISWNFKHIVHFEKIPMYNAVNILNGYGPIIIFSPMEVIENEEF"
     gene            complement(33978..34406)
                     /locus_tag="PK937_00140"
     CDS             complement(33978..34406)
                     /locus_tag="PK937_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31012.1"
                     /translation="MILSVLNVVWPAIYILKGIFAFWYVVVMTIFIEAFFLKKMIGIS
                     QKQSVIMSSVGNICSGVIGTAVMAFTMIPVTGYGPHFLSDLNSKANWILSFVVMFAGS
                     ALIEITVVRLIWKQSIGKTSAALLIGNAITYITIYFSIQF"
     gene            complement(34403..36955)
                     /locus_tag="PK937_00145"
     CDS             complement(34403..36955)
                     /locus_tag="PK937_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH31013.1"
                     /translation="MQSLVDTYVIAGNGSATGKKFYEERSSLNYSTNHGYTANQAFPD
                     LASCDVWTVTYYDDYNFDNTGGDDYTFASDIEFPNNSKFDRLKGKVTGAKTKILEGVQ
                     GYAGFASAPDTVVLKNQAQSSGNISAKEIGLEGEVMLTGEMTLEAKKDAVKQPSSYAA
                     ALNGVTWLSSVSFYDKYGRVIQTQSNNSLGGTDVSNTEYDFPGKVLKTKTVHTTSITT
                     VTVKKRFDYDDGGRLIKTYQQNNTDSEVMLAKNVYNELGTLIEKDLHSTNSGSTFLQA
                     VNYKYNIRGWMTKINDPANLGTYLFGMELKYESPDAAIAGNAQYNGNISQQIWSTSLT
                     SRHAYGYIYDKLNQLSEAQYESYSGATWNQTPGIYNETMTYDRNGNIKSLLRAGKTNW
                     SSNTYGTMDNLQYGYIGNRLYSVNDAVADIANNQKYDFSDNGNELTFSASNASTHEYS
                     YDVNGNMKEDKNKGITNIEYNYLNLPTKVEFGADHQNRIEWTYTVSGVKLEKRVYTSG
                     ALTLTQDYVSGFVYKNGVLEYFSNTHGRLKRKPNGSLRYEYMLSDQVGNTRVTFTDDD
                     NNGQPEIQEESHYYPFGMRIEGISSNALDNKLTFNRKELEDDLGLNWYHYGARFYDPQ
                     IGRWHVVDPSDEFHSPYLALSNNPIITVDPDGRDVIYLLDQNAPFGTGLSGHTALLIG
                     NDETGWYLFSKNGFVDGKESNTDGQYFSNLAAFEKEGYWGYEKGAYVQTTHEQDQLLL
                     ASARRNVTKKYDTQDNNCDGFCRSTLSENRMKTAGSEKFLGIIWPKEGFEDFVKKMSA
                     QVLNLHMPDNYSPIVGHNFVVRDREGIVESGPFRTNQWYDPNTDTWKDWGTK"
     gene            complement(37303..37986)
                     /locus_tag="PK937_00150"
     CDS             complement(37303..37986)
                     /locus_tag="PK937_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH31014.1"
                     /translation="MVKHLSLNKKILYLFFLGNIDVLINHFYGPKFYHEHPEHIFAFQ
                     ITLLVIPFIVLIYAIKNTSLTWKSIGITTTIGNIKKSSAKYYYVFSFFILILLVEAIS
                     TSIVKFLIPINLLAMNPVEIPKKSVFVDFLVGFLIAIRSGFFEEIYFRGFLNLFFANK
                     ITFVIISSLLFGFMHWPMGLHSVVTSVLIGIVYSINYIRTGSLVPSIIAHTVYDLLLE
                     MGKVFLGAA"
     gene            complement(37980..38357)
                     /locus_tag="PK937_00155"
     CDS             complement(37980..38357)
                     /locus_tag="PK937_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31015.1"
                     /translation="MKSIGLRVQSNDNGSINFSMRLGNGATVHSPGGQGVNIMKNDKS
                     IIKDFGSGFNYTVKLQYKPSLGLMIMEDLFQKYLERSGLKVPLTVKKDPFVPRESFTP
                     KNYVKPVTPETIRNQMTSPGLTW"
     gene            complement(38501..39217)
                     /locus_tag="PK937_00160"
     CDS             complement(38501..39217)
                     /locus_tag="PK937_00160"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNH31016.1"
                     /translation="MEREEISRMLAQDFSFRQMTMRLSSETIYTYIYILPRGTLKKEL
                     ISCLRQNRKRRYRHTRGAKPVRNLEDMLSIEERPQSLEDRIIPGHWEGDLIVGKYNRS
                     ALGTLVERTTRTTILVPLKNRNAYDLAKAFAKEVIKLPQQMKLSLTYDQGREMAEHKL
                     FTHITGMKVYFAHPRSPWERGTNENTNGLVRQYFPKGTDFNNVSRYQIKKVQNLLNGR
                     PRKVLNFQKPYEVFNQLINQ"
     gene            complement(39284..39982)
                     /locus_tag="PK937_00165"
     CDS             complement(39284..39982)
                     /locus_tag="PK937_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31017.1"
                     /translation="MQRHINGMDISMISKKLLKNTIRFTPFLLMCLLIVFSSCEEKKL
                     TINFIQDELIVNSNSDTLSTIVYLINRSNDSLIVYTAFNKTIPRPEVFGNGPLMESKY
                     DCRDVVLNGFNVLLYDSAQEMCLVRDLPYHFDIDTLLNNKAIGSNSINSKRQHESTNT
                     YDDILSSRVRLLPSDTLQVSIQIPLNRYSLSNGGYWLRLIYFVNTNSFPHSLYDSNVF
                     DGCVFSKMIKVFIR"
     gene            complement(39915..40469)
                     /locus_tag="PK937_00170"
     CDS             complement(39915..40469)
                     /locus_tag="PK937_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31018.1"
                     /translation="MLRVKELLKQVILIPKNLLKLAKKTSKAIKNSEELQKLHKAVGN
                     SDVRITTPENLKAVGSRRIGVIDGSMYDLKDGQMSAGYTGVIQRSGFKTSDGSIGFRT
                     YNIISRSAFASAEKLFLTLGHELTHSIHYFSGEYVRWGNSQNSDDAHNMSEKAAYAWE
                     LNYATSHKWDGYINDIQKALEEYD"
     gene            complement(40689..41318)
                     /locus_tag="PK937_00175"
     CDS             complement(40689..41318)
                     /locus_tag="PK937_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31019.1"
                     /translation="MKQKTFKLMQLFSIAASFYFITGCVGISNSRFSKLDLENNNFNK
                     IVEEPYVGEMIVEKNFAYRVFPLNAADSGFLIFPIPFPFSESTPIDSEFSVGISLKPF
                     CTDLRFDPQKVIFWTDSSTKYLPYGFIGPYECSSSDTRPIERKLPVEPIMLEDSITSC
                     FWIKFKTIVPDPKKKLHFEIKGLSINGSEVVIPEIVFKQAKRRELFSLP"
     gene            complement(41553..41978)
                     /locus_tag="PK937_00180"
     CDS             complement(41553..41978)
                     /locus_tag="PK937_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31020.1"
                     /translation="MKILIVIVLLRILSCDEEKNSYSTMGRNHYIFEFPGKIYGRTII
                     GFIETYDSIAIKTSFEISYSKDRIIHVNKITDSSQYKYSVNAEKNVYVHGPRITCYDT
                     NNFKNNKIHKEAFYRLIADDFLSKTNKEKHDFLLELSSK"
     gene            complement(42340..42531)
                     /locus_tag="PK937_00185"
     CDS             complement(42340..42531)
                     /locus_tag="PK937_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31021.1"
                     /translation="MSDKVICCFCGKTLPLEEATILTIQPNIKSGEKQNLFCHKNHLM
                     ERLIKSIPLHPDLFDDDDK"
     gene            complement(43193..44389)
                     /locus_tag="PK937_00190"
     CDS             complement(43193..44389)
                     /locus_tag="PK937_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH31022.1"
                     /translation="MVLKSSFQQKGLRVGRDKLFSVLRESGMLIKPKRRYVQTTDSRH
                     WVRKYPNLISALPPPTRSEQLWVSDITYVASDEGFGYLSLITDAYSKRIMGYAFRSDL
                     TKEGPLSALRMALKGRRYTDQALIHHPDRGYPYLCASGNITSSSPKGIGTLTYDPFIH
                     RTRTIAIGASSMNFQYDGTERRIYRKEVVSGVTSEALYLHDGMNAIHERMKTGTVTEY
                     VYGPTGIIAYGSASTWKYVIKDHLGSTRAVVDGTTVTGYDYSAFGSILAPVTPNTNYM
                     YTGQEYDKTSGLHNYKARMCDSDLMRFYGMDPAGQFSSPFLYVGNNPALRNDPDGKIA
                     PLIAGLIIVGSGIYNAYQNRDKIDNFGKGFSFFLVGGGQATASIFGWPTGAIIGGMLG
                     SGANSA"
     gene            complement(44534..44938)
                     /locus_tag="PK937_00195"
     CDS             complement(44534..44938)
                     /locus_tag="PK937_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31023.1"
                     /translation="MQEGQGCRMYVKRTQRDYSLAFKLQVVDAIEKGDFTRKEALSKY
                     GIQVQSTLHVWLCKHGRLDWSSTMQKKATPGKRIRELVKALERAREDKEILQRAIDIA
                     EAQLGIDIRKKYLAQLSEAMAIKGAGDTALGE"
     gene            complement(45043..45192)
                     /locus_tag="PK937_00200"
     CDS             complement(45043..45192)
                     /locus_tag="PK937_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31024.1"
                     /translation="MKKLMLVAAAALMVTSFVFAGMGQDNCGSCPNKENCGTKVCTPK
                     ECCKK"
     gene            complement(45775..46770)
                     /locus_tag="PK937_00205"
     CDS             complement(45775..46770)
                     /locus_tag="PK937_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNH31025.1"
                     /translation="MKTQSRWVYVFSFLYPLSVVAGNTLGGHFARSGAVVGLLIYPLL
                     DWLLGESESNNLEDSDPFYFDAILMTHAVLQIVCLLSLALLIQRDGWNYFAVLASIST
                     GLNSGISSIVIAHELIHRRYAFQRVTGVFLLWTVNYMHFYFEHVRGHHKSVATVEDPA
                     SARENENFWSFLIRTIPGQFINAFKIMSPRANSWRLNPVLVFLVIQIVSWIVVYFLLG
                     KFILFAWLIQSAFAVFLLEYVNYIRHWGLQRPVGSRVQPEHSWQSNHRWSRWTLVELT
                     RHADHHYIASREYWKLRTYRESPNLPTGYYGCFWLVVIPPIWRKVMKKRLPKQGI"
     gene            complement(46805..47968)
                     /locus_tag="PK937_00210"
     CDS             complement(46805..47968)
                     /locus_tag="PK937_00210"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNH31026.1"
                     /translation="MKRIGVLTGGGDVPGLNPCIKALVYDAIHAGMEVVGIRSGWGGL
                     LRYNPDDPEGSKKWITPLDLINTRTIDRSGGTFLHTSRTNPGKVNKSDVPDFLKKPEH
                     DTLDSKTKIDFTPHIFRVLKHLKIDALVTIGGDDTLSYSVRLHNEDFPVVAIPKTMDN
                     DVYGTDYCIGFSTAVTRSVHFINMLRTPAGSHERIAVVELFGRNSGETSLISALLANA
                     DRAIISEVPFDPEKLSEMLLNDKRNNPSNYAIMTISEGATMEGGKIIEYGQEDAYGHK
                     KLGGIGEITAEAIKKITGQHMIYQSLAYLMRSGEPDALDRMVAMNFAGLAIDLLTKNR
                     FGMMTALQDGRYTTVPAETIIQGKRNVDVKELYDVENYRPKVFNVMHKPMFLY"
     gene            complement(48112..49341)
                     /gene="xseA"
                     /locus_tag="PK937_00215"
     CDS             complement(48112..49341)
                     /gene="xseA"
                     /locus_tag="PK937_00215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNH31027.1"
                     /translation="MDLFSVSNENSSDLGQRQEKKPVIYSITEITRLIKTHLEDSFPA
                     ITVSGEISNLTRASSGHVYFTLKDAGAQIKAVIWKSSSASQSDALKDGKKVIARGNIA
                     VYEKGGYYQITVQKIQQEGIGALQEAFEKLKQKLYAEGLFDTSHKKTIPKYIQRVGII
                     TSPTGAAIRDILSVARRRMPWIELILYPVKVQGEGAAEEIAHAIHEFEKYGAVDVLII
                     GRGGGSLEDLWAFNEEVTARAMYACSIPTISAVGHEIDISISDYVADYRAATPSAAAE
                     MVTVDQEDMRSYIVNLQYTLSQNVKKRISDYQKQLHNLKNRYAFRQPENIVGQYRQQV
                     DDFEKTMMRVLRHKLIVSGQTITALARQLQTLNPKNTLQRGYAIVRQKDQIVMRASEW
                     DDTKPTELEFFDGKKNT"
     gene            complement(49347..50006)
                     /locus_tag="PK937_00220"
     CDS             complement(49347..50006)
                     /locus_tag="PK937_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31028.1"
                     /translation="MSSAKRIGCFIFIVLFTVCGLSADAQVRRKPTKKVEPKKETKKE
                     KGKTSYLIGATTSYSGITGVVVEAVGKSNKSTYSLEAGYSFRRVKFESPYIVLDTSLT
                     NKTEMVHGFGAHICMNNYLSKYKQGFAWTLGVGGHYFFKKHDAKIYNITDEPYYRPGP
                     DVKFFSVFGRVSYRLETDEGMYLTPFIGGGIMATPFKSTVHSKINGAFFQGGLNFVMK
                     L"
     gene            complement(50003..50695)
                     /locus_tag="PK937_00225"
     CDS             complement(50003..50695)
                     /locus_tag="PK937_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNH31029.1"
                     /translation="MRKSEKRIISLCITVLFSLSFTVHAGSGDEEKKVVISSMRTANE
                     ATISLQAKMKQRKQSSFMDKEIVTKADFYYQKPGKYALNPSSEAENQYIVNNNSIWII
                     NRKNKTVTTTSDNEINFSQYLLGFGNSLEMLEKYFDVQVDTKQVQKKFGSYKLTLVPK
                     RGSKFYDKMESVIIYVRDDLWLPYQAELSEADGDKTIWEFSDFRLNHSINSDVFKQET
                     PKGFQVKNLEKK"
     gene            50972..51523
                     /locus_tag="PK937_00230"
     CDS             50972..51523
                     /locus_tag="PK937_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH31030.1"
                     /translation="MALRKLAYDEIKQLRSTPEELVYKTRFPIYGVLENIRSMYNVGA
                     AFRTSDAACIQELILCGYTAQPPRSQIEKTALGATETVPWRHFNNTLDSINYLKDRGV
                     TVVALEHCEESKNLLTTEFPFPVALFVGNEVDGLNEETVAHCDMACEIPMFGSKQSLN
                     AAVAYGIAVFELTRKYLVLQEKG"
     gene            51528..52115
                     /locus_tag="PK937_00235"
     CDS             51528..52115
                     /locus_tag="PK937_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31031.1"
                     /translation="MRSLMVLFLFINLTIYGQEFSIGWTKASTESGSEFKKYEPQTAC
                     FFIKHRRLYNEDFMWGLGLDMMSFRKSASPERTIKIKGITGEIGYYFRQIPGLTVLGS
                     TSFGLMRCEYDFIDPPADLKGSSGTGVSIEPSVSIFFPLSKTLIFSPSIGYHMLFDKM
                     NFKTTNPIKQPIGKLDSHMFAMTYRLALSWRFRKE"
     gene            52120..53100
                     /gene="nadA"
                     /locus_tag="PK937_00240"
     CDS             52120..53100
                     /gene="nadA"
                     /locus_tag="PK937_00240"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNH31032.1"
                     /translation="MIFKKNTYSNDEINIQRISPDIDLFKEINRLRKDLNAVILAHYY
                     QESEIQDLADFVGDSLELARQAAKTKADVIVFAGVHFMAETAKILNPEKLVLLPDLKA
                     GCSLADSCPTDEFKKFREAHPDHFAITYINCTAEVKALSDLICTSSNAERLIAQIPES
                     TPILFSPDKNLGAYIRKKTGRPMILWQGSCMVHEIFSEKKLVQLRATHPEAKIIAHPE
                     CEETVLNMADFIGSTSGLLKFVQQSPDHTFIVATEAGIIHQMEKACPDKTFIAAPPNN
                     NCACNECPHMKLNTLEKLYLCMKNKTPEITLPEDVRTRALKPILRMLEMS"
     gene            complement(53131..54201)
                     /locus_tag="PK937_00245"
     CDS             complement(53131..54201)
                     /locus_tag="PK937_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31033.1"
                     /translation="MRSDSIEMEFELLGDNGNVNIAKERIYGPIKNYTDFYKTMYRAV
                     ENLIQRINLTEKNILSPNGVLLGHERLRTIASDHEEYALYVQKWEALGYYSLGVSAFE
                     KNNDSDAILFFEKARRLDYNNLLKSEKNISAVYLRAANMAYRNSHWPSAITGYNRAIE
                     FQPENADAVFNLGNVYQARKEWDVAVLQYRRALELNSKYVDAWVNLGVVLGEKGNAPE
                     ALVAYESALKIDSLRALVHYGCAVGYDDNLDYVKAEIHYRKAIVLDSNLYDAYLNLGI
                     LLKRKKQHKEAQSLFEKSLALNPNNANIHRSLGIVYMNDKKQIKKAIHHLEQTLALDP
                     AQRDADVIQKNINVLKKRLSKK"
     gene            complement(54508..56040)
                     /gene="zwf"
                     /locus_tag="PK937_00250"
     CDS             complement(54508..56040)
                     /gene="zwf"
                     /locus_tag="PK937_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNH31034.1"
                     /translation="MAAHVTEALRQVKSDARAGTCSVIIFGGSGDLARRKILPALFNS
                     AKEGLLPEKFTVIGCGKPEMSSEAYREMVSSALREFAKSDAVDPKIVNEFVQNSFYVG
                     GGFDENALYQTMDSLIKNNCTTRACNDNRIYYLATPPTVFATICQKLAEHNMSQVRGG
                     NYWRRIIIEKPFGRDLESARALNAEVAKAFEEWQVYRIDHYLGKETVQNVLAFRFANG
                     IFEPLWNRNYIDHIQITAAEALGVEDRGGYYDNAGALRDMIQNHVLQVMALVAMEPPT
                     TFDANAVRDERVKVMRAIRPVKPDQVNDYFVRGQYGAGQINGKPVPGYRTEPKVDPQS
                     NTETYVAAKFYVDNWRWAGVPFYLRTGKRMPRRDSEIAIFFKRTPHMMFTQTAADDVA
                     PNVLVIQIQPDESITMSFEAKVPGPEMQLQSVDMHFNYKEAFQVEIAEAYQRLILDCM
                     RGDATLFMRKDMVEVAWWLVMPVLEHWKNNKAEFPNYVAGSWGPEAADKLLNKDGRKW
                     RS"
     gene            complement(56108..57286)
                     /locus_tag="PK937_00255"
     CDS             complement(56108..57286)
                     /locus_tag="PK937_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH31035.1"
                     /translation="MRFIWFFLTWTAIIMSGYWYVGRRIVPAMAHFGLPVYIGWIVIA
                     VLCLLPLGGFMMVLQRQHESISLDVLLWCGYSSLGLFSLLLVAFLLRDAGGWLHHGFT
                     WLFTKPNETVTDETRRQFLIQSVNLGVAGFCTAVAGYGIFKAHNDPVVEAIEVPLQNL
                     PKNFDGFRIVQFSDLHVGPTIKREFVQRVTDHVMALKPDLVAFTGDLVDGTVAWLREE
                     VEPLKQLQAPHGVFFVTGNHEYYSGAKAWCDEAKRLGFDVLLNEHRLIHRENQQICLA
                     GITDYGATDFIPGHLSDPVKAIDGTSSDYSRILLAHQPKSAPAASAAGFDLQLSGHTH
                     GGQFFPGNLLAAMAQPYLKGLHRHDNMWVYVNRGTGYWGPPMRIGSPPEITVITLRCA
                     "
     gene            complement(57377..58981)
                     /locus_tag="PK937_00260"
     CDS             complement(57377..58981)
                     /locus_tag="PK937_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH31036.1"
                     /translation="MNQRSLFQIVLAVALVFTTACKKNDAVSDATGDTPQYGGTVIYG
                     KNGPPVTLDPAFVEETESSIIVGNLFEGLIIQRAGKIALDPGLAKSWKISLDGRTYTF
                     NLKTGLTFHDGTPFNAQSVIFTFERQKSGGEGESSSGYALWKNFNMDNIFQSIEAIND
                     STVKIVLQKADATFLNILSLNFLAIVSPAAVQKYGAEFSKNPVGAGPFKFVSWDTDGS
                     VRTAAFDGYWHGRPYLDTVIFRPVPDPHERWLALKSGVINMMGLPTQKDLADIEKTPG
                     VKVTKQPGMNVAYVGMNMRKKPFDNPKVREAIVLAINKERIVREVFAQLGSQAKNPVP
                     PTLLGHNDQIQAAPYDPERAKQLLKEAGFPNGFKCKLWGLPLVRDYMPNPPLAAELII
                     NDLKAVGITAEEEKISNWGEFLRRRGRGEHEMSVSGWVGDAPDPHFFFYPLLDKDITK
                     QQSSTNVSFYASEEMHNLIKRGRQTFDPNARADIYKKACEVFNKDFPWVTLAHALTIV
                     PMRDNVMGFELHASATRRFEKTWIKQ"
     gene            complement(59041..60600)
                     /locus_tag="PK937_00265"
     CDS             complement(59041..60600)
                     /locus_tag="PK937_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH31037.1"
                     /translation="MFFFVSGCSKKENVNTVSDNQPRFGGTLLYGKHGPPVTLDPAKV
                     AETESSILTDNIFETLVRLQPGNVDVVPGLAKSWIIAVDGLSYTFNLQSGVRFHDGTP
                     FDAKAVLYSLDRQKKGARRSADQFSNWINFNMDELIREVRAVNDSTVQFKLHRADATF
                     LKILSMQFLAIVSPAAVEKYGADFEKHPVGTGAFMMSASSDWNDLSLVANPNYWGERP
                     YLDTIRFLSEPDPEKRITRLEQGEFHLIEPPPFDMLARVKRNEQIIQFKQPGANVSFL
                     AMNMSKKPMSDIRVRKAVAYAINREKLVQAVYGSFGVPAKNPIPPGMLGYNDDLRPTP
                     YNPAESERLLKEAGYKRNLKLKLWNVPLPREYMPHPEKAVEMIRSELEKVGIEIEVVT
                     PPWETYIQDTYLGDYDLLIGGWIADIPDPDNFFYPLLSKTTVRQKGTGNSAFYDNDEM
                     HRVLLKAKAATKTEERDQLYKNACQIFQRDVPWFTIAHTQIVIPRRREVMNFNPYASY
                     TRNLKNVWVKS"
     gene            complement(60663..63434)
                     /locus_tag="PK937_00270"
     CDS             complement(60663..63434)
                     /locus_tag="PK937_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31038.1"
                     /translation="MIDIKHILTVGVDDQVKTIYEDYIAQLTERIGISDANAKPAFKA
                     LMNILITLEESGNIRKGYDSLPSLFITQRVLYMKVFDLMLQHGHAVSDVDEFWFRGKE
                     IELASPSHFIDDQVQVARSIFSVFQPFLDADDDVDRFMRVISLYAAFISKKNDVGAMV
                     NLMHHFAHFADKIYLAGYKKVHDGWGGKLDLDTDVYPVFFKSIIRDAIETYGFSDYVV
                     DFLYDRLKEVGESETVLPDLYDFTKSLQANAELTKEIQDADKSLKTEIQKTLQIVQQQ
                     KGESLSENAQNAIVRELTAYEELEGLIYYYFDERRLFKIMTDTKTVSLFEKTLDIVRS
                     YSPKMKHYVMYAFGKLLGAVKNNVEVDEAVVEMFCREIKLDIETINSDKVMQNLPAQY
                     RARVPDLEVRFVFNRMMSNTASLEGFERVHDLFRAYLTDQTDFPLIHEMFIERAAEKR
                     HASALMSFYVAALLVHELGDIVSDQLIKVLAGRLSLTYSFTRRLTLFQEIKKLRYEFD
                     GSHTGRETLYYNGFDKQNAVVPEKEKQTPLKSRPFEEQWIILYHYLNNRELAQALRLL
                     DDQILFDYIYKREHTQEEIDVARTELRQKVYKIIDDKFLDVVSNEEDREKRELESEGK
                     FAAGYYGKYEKVRSILVAQFVASGWDMEVHELRRKGKRPLIKMTQTELLRGIKNIDPN
                     RILEQYEYKEARENIDVSTLRKQVDARELLLGFVNRTIKDETAFYQKALGLSDEAVAL
                     LSYSSTKLRSNELRVELGVRQLVERLRADLGVAAQREPLPLHDILQKAFAGTYGKIDV
                     KVIKKDKNGKPMIDPETGEEAEKTYTIEPGKFPDYLKSARNEIEALGKSELYRQFYLI
                     TLSSILSETFSSMTFIKEALATNKYVIAADGLKEQSLENQEIIEYARIKMDSAAQLMP
                     FELQLQPIS"
     gene            complement(63431..65251)
                     /locus_tag="PK937_00275"
     CDS             complement(63431..65251)
                     /locus_tag="PK937_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNH31039.1"
                     /translation="MDNGSPRNLADVFHQSARRFGHKTLYMSKRDGKYRALTYGQVHE
                     IVCNLALGMASLGIKKDDKVAILCPTQEEWAMSDFAILSLGAVTVPIYPNLPTPQVEY
                     ILQNSDARLVVASDREQLAKIIECKNQCPMLNNAVLITHEDKHKDYVLFFSELQELGK
                     KFGTENPGYIEKQIDSIDPKGLATIIYTSGTTGMPKGVMLSHNNILSNSLAAVKVATV
                     GETDTMLSFLPLAHSFERMAGHFMPVIRGCAIAYAESIEKVPQNMGEINPTVMTAVPR
                     LYEKMYDRVKEGLSTAPPIRRKIFEWALGVGKSAYETGERGFKYKIAEKLVFSKLHHR
                     LGGRLRFFVSGGAPLAKEIAEFFAYMGVMILEGYGLTETSPVITVNRPEKVKFGAVGP
                     AIDGVEVRIAQDGEVLTRGPHVMMGYYKNEEATREMIDSDGWLHTGDIGFLDSDNHLH
                     ITDRKKNIIVTSGGKNIAPQPIENTLITIPFVEQALVIGDRRNFISALIVPKRERLRD
                     WAMQHGIKFDSEEELLENKEVYAMIDKLIQEAQDKAGFARYEKVRKFALLPKAFSIES
                     GELTPKLSIKRHVVTKNFKDMIDTIYNRETERMTEGDSLT"
     gene            complement(65403..67034)
                     /gene="bshC"
                     /locus_tag="PK937_00280"
     CDS             complement(65403..67034)
                     /gene="bshC"
                     /locus_tag="PK937_00280"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNH31040.1"
                     /translation="MTISFRELKNTTSLFTDYLYSPEKTTSFFSANPSDQKAWSDTFS
                     EVLKKYKTNRELLSDILADQQHRFQSAKQALENVALLRQPNTVAVVTGQQTGLLGGPI
                     YTVLKALGTVKYCRLLKTKFPDYNFIPVFWLELEDHDFEEVRSTQLINIQNELVKIFY
                     DGGISENQNKTPVHRFALTDDISRIIQELKTALNPTDFSQALFDTITKSYQPGKSWAE
                     AFGMLLSSWLSPMGLVLMDPSDVRFKGIVKPVFERALKESESLHEAVLNQSNALKVAG
                     YHAQVETEPSYVFLTNYGENKLSFNNKGHDKIRITRDIWKTYKNDILHILENEPQRFI
                     PNVILRPLIQDTLLPTVFYVAGPGEIAYFAQFRDLYTHMDMASPVIIPRPFFTLVEKK
                     IKKVLDKYQITIADIINDGEAVIERINQQSNVSLEPALLRFMNEVQDGMKNLETQLTA
                     IDASLKGASATAMDKMQQAMQVLTQKTRDAEKRAALTTHGQLTKAMLHIRPNDNFQER
                     ELSILYYLNKYGFTLFDTISEQITLDSWDHRLIEL"
     gene            complement(67039..68793)
                     /locus_tag="PK937_00285"
     CDS             complement(67039..68793)
                     /locus_tag="PK937_00285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH31041.1"
                     /translation="MKKILYIEDDPVAMTYMTQLIQRMGHTIITATTGESGLAKAFAE
                     RPDLILIDLLLPGLDGFELTIKMRSSETLKHTPIIATTATPNEDDQQLATMAGCDGFL
                     VKPIEYEALSDLIRGCFDSETHTNKNVSLESENIELRRFTLRLVHRLTDKINEISYAN
                     KELQKTNNTLDRLIQEVRTNNADLMQINQLTSQIMAYRDRSTIYREMPQLISDQLSLA
                     GAAIYVVNEKDLTLDVFSSYKYDFLPETEHIPFTEPPFFETVYDNQSVTIDVHKLIAL
                     QRENERHARRLEVLFESFHAQTVCFVPIRGRARAADDFECENQDCEAFVKKDGQWWRN
                     LIHQYNRRDLDYPSRLRDASRYFFNCCFYDLKGVLALGIPDGRMSDAFRQIIHSFTTT
                     VGLRLDNVQLYEDVREAYMLAEKQAITDAMTDVFNYRYFHHQLEREIRRAKRHWSKLS
                     MIMIDVDHFKSYNDRHGHPAGDEILKRLAVVFKNITRTSDIIARYGGEEFVIVLPETP
                     KVAAVKFAEKLRAAIQDEDFPYEQTQPMGDITVSMGVATFPDEAQTIDELIQRADEQL
                     YRAKSGGRNRVAYSNTTG"
     gene            complement(68815..70182)
                     /locus_tag="PK937_00290"
     CDS             complement(68815..70182)
                     /locus_tag="PK937_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNH31042.1"
                     /translation="MKDKIFIANGQGFWGDSIDAPVHLVQGGQLDYLTLDYLAEVTMS
                     IMQRQKLKKPELGYATDFVQLIERILPTIVEKNIKVIANAGGVNPEACRQALFAVAKK
                     LGIKGLKIGVVYGDNILPRISEMETKQIPMKSMDTGESLYDLMKQGRDILSANVYMSS
                     LPLVEALQKGAQIVVAGRTTDTGLAMAPMIYEFGWSFDDWNKLAAGTVAGHIIECGAQ
                     CTGGNFTRWQDVPDMWNIGYPVVEAYPDGTFYVSKHENTGGMVTVDTVSEQLVYEMGD
                     PHNYITPDVIADFTSIRLEQAGKDRVKVFGVQGKPSTEFYKVSASYLKGYKATGQLTI
                     SGPDALPKAKLAAEVLWKRLTRAGMEYEETHTEFLGVNTCHEGMVAIPNPINEVVLRV
                     GVKDKDQKKVDRFGKEIAPLVTSGPPGVTGFAGGRPKPQEIVAFFPCLIPKHLIETTV
                     SVEEV"
     gene            complement(70293..72761)
                     /locus_tag="PK937_00295"
     CDS             complement(70293..72761)
                     /locus_tag="PK937_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH31043.1"
                     /translation="MIAVKHDKRFFIRNTLIAVGFGLLSIGMSYIRFVIPTFNFMTSS
                     SLNEVAILISILFLPHWGFVIITGAMTAVNAANELQLFSAVSHGFSAAMAWLVYHRFF
                     SRFTKSRQEPILWVMVVILYYYLFLLPQIVGLYLLFDPNPMPAMHITSAFFVNMTPEL
                     FATLLITLLFSIMRQELLSRRETERALAEKEERYRIIAEQTGQLIYDLDVTTGAITWT
                     GAIEINTGFTKEEFLRVDLKTWETMIHPEDRPNAISLLDEAMASGNRYHVEYRFRKKD
                     GGYVFMDDNGLFLRDEKNTVYRMLGTMTNIDERKKAEKSIKENVERFEIIARATNDAV
                     WDWDARQDKVWWNESARKLFAVEEYYPQDNYRLWQERLHPEDRERVTQKYKNVLESTQ
                     NFWSDEFRFRLPDGSYGYFYDRAYILRDEQGRPTRVLGSMTDMTERKKLEQNLIQAQK
                     TESLGRVAGGIAHDINNMLAVILPTAELLLNQNYDKHTVHQHADVIVSSARRASDIVK
                     QLLVFARQTPSSAALLNINNLVDETRNMLDRFLGKNITITLHLEPEIPYIDADATQVQ
                     QIIINLCVNARDAMNNKGSIGIETKMVELDDTESAKYNVPEGLYVQLSIADSGTGIPP
                     EVMDKIFEPFFSTKDIGKGTGLGLAVVKTIITNHGGYIHVHSQIGVGTRMELGFPVSQ
                     RVTIPHVQAQKPQYETGTGTILLVDDEKPILEMGEMVLKRLGYQCYTAENGYRAVEVM
                     NQKPVDLVILDVQMPGMDGIETLEKLLEIKPELKFLFTSGYIGADRLQLLHSKYPDHI
                     VNKPYTVEELSKAIKKLLDTQSAG"
     gene            complement(72875..73099)
                     /locus_tag="PK937_00300"
     CDS             complement(72875..73099)
                     /locus_tag="PK937_00300"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HNH31044.1"
                     /translation="MERVGGAMTSDYKEIVFTITRDGSLEYAIKGYKGGACEDLSKLF
                     ESMGAVIEEKKTYEYYEKDEDVTQKIRLQS"
     gene            complement(73087..73461)
                     /locus_tag="PK937_00305"
     CDS             complement(73087..73461)
                     /locus_tag="PK937_00305"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNH31045.1"
                     /translation="MSKFVSLKTALRERVHLENALKEMGCEISNSKKIKTLLGRSYDV
                     DLTAKTAFGMIGFIKNRSGEFEIAGDDMVLASDKDFVGKLTQKYAYSRVVSEAQKAGF
                     QLVKESVNEDQSVRLVLRKWNG"
     gene            73561..74121
                     /locus_tag="PK937_00310"
     CDS             73561..74121
                     /locus_tag="PK937_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNH31046.1"
                     /translation="MRKWIIVFLSLACLFFYLKNYDISSVEGRSENHGVIIKISDGDT
                     FLIEVNGKTEKVRLIGIDTPESKDNLKAKKDAARNSADLDAMVRQGIEARRFVERNFP
                     LGTILRIETDRDTRDRYGRMLAYLFTEDDTMINETIIREGYATPLFYSPNYKYKSRLE
                     EAYRYAKSNKKGLWQNGKPLHIRKDD"
     gene            74230..75324
                     /gene="ychF"
                     /locus_tag="PK937_00315"
     CDS             74230..75324
                     /gene="ychF"
                     /locus_tag="PK937_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNH31047.1"
                     /translation="MPLRCGIVGLPNVGKSTIFNALTAAGALAANYPFATIEPNVGVV
                     TVPDHRIDALIKVVKPKRVLYTTVNFVDIAGLVKGANAGEGLGNQFLSHIREVDAIGH
                     VVRCFDNADVVHVEGSVNPERDIAIIDTELVLKDLESVNKRISQVERTAKSGDKDAKL
                     AFDVLSKVKVQLNDGKPVRLMSLSENDRKAIADLFLLTDKPVLYICNVSESDVLTENN
                     YVKQVRSIAEKENARVVVISGQIESEIQQLPEEERKGFLESLGLKEPGLHALIRETYS
                     LLDMISFLTAGVEEVRAWTITRGTKNPQAAGVIHSDFEKGFICADVIWWEDFVKHGGE
                     QACKDKGLLRTEGKEYVVKDGDVMHFKFNV"
     gene            complement(75355..76320)
                     /gene="obgE"
                     /locus_tag="PK937_00320"
     CDS             complement(75355..76320)
                     /gene="obgE"
                     /locus_tag="PK937_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNH31048.1"
                     /translation="MFIDFVQIQVRAGHGGDGCVSFRREKFIPKGGPDGGDGGKGGDV
                     VFEADENLHTLQDIVHHRIFRAQHGQKGGSQNMSGRFADDIVIKVPVGTIIKDQETGE
                     MLCDLTENRQRAVLAKGGQGGKGNSRFKSPTNRTPREFTPGERGEEKFLDLELKVLAD
                     VGLVGFPNAGKSTLLASVTAAKPKIANYPFTTLTPNLGIIKIGEYRSLVLADIPGLIE
                     GAHEGKGLGVQFLKHIERTKALAFLIDVQNANPEKDYVTLKKELKAFNKELLNRPRLV
                     VLTKIDTVDTLPKRTKMKDGTPVLMISSVAQTGLEKLVKTLWKML"
     gene            complement(76347..76913)
                     /locus_tag="PK937_00325"
     CDS             complement(76347..76913)
                     /locus_tag="PK937_00325"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNH31049.1"
                     /translation="MNIVHNSGWIEVIAGSMFSGKSEELIRRMRRAEIAKMRVQVFKP
                     QIDSRYSEDHIVSHSELKMPSEVIEKASEIMEKLDPRTDVVGVDEAQFFDASIVEICN
                     KLAYMGKRVIVAGLDKDYLGRPFEPMPQLLAVAEVVDKPMAICMVCGAPANYTQRLVQ
                     SADRVLVGATGTYEARCRRHFDPPKDTK"
     gene            77047..77946
                     /locus_tag="PK937_00330"
     CDS             77047..77946
                     /locus_tag="PK937_00330"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNH31050.1"
                     /translation="MNQQITINGVGIHSGKPSRILIRPHEHGVLFNVEGTPIPALIQH
                     VKDTQRATTISVGDRSLRTIEHLCSAIYGLNLSGAEITVTGGEIPILDGSAMPFIEAL
                     ADTLTTESQNEALTIDRTITHRNPDNGAFIRIEPSQNFHVTYTQRFQKRAIDTFSFFS
                     SPKNYIKEIASARTFAYAGELQLLYRKGFIRGLDPNAGFIILDSEPNWDLMEEIIGED
                     ARSAVHETPFYKILSKIPPRYPNEATRHKVLDILGDFSLLGMPIKGLITADGTGHAEN
                     IALVQKIAETYGLIHFDPLGDDE"
     gene            complement(78013..79473)
                     /locus_tag="PK937_00335"
     CDS             complement(78013..79473)
                     /locus_tag="PK937_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31051.1"
                     /translation="MKRLWTLLLLVFMIACEKEKSDGTLTDDQKEVVEAAVKTTALVS
                     QVDDEFSDNEEITSENGYNGAGDESDYTASSIIKDISDEHLMKSSSSFTFVGRDTITR
                     NYPDGTQATIIRVFDPTTLRLTSIDVATKNFPNSSRVEKKFYRATFTYDPNVIITQNT
                     VMTSSFKDSTIIKTYEEYPGFTQITNGTIIRNVGRVKFADRKLSYSATTTGFATVSGI
                     SNRVTSISIDPVGGTSHKETTTNYTDASSSTSEITYTRTVNATTGERTLSGTMDITLR
                     DGTHRYSVSTMNITADSSNLSKSGTIQIQTDFPAGDSLYRKITKVIMSGAVSTDSSTF
                     YFLDGTTYSTVLTITRTSAPTGNGTPEGLSYTITGSDSRGRTLNGTVTGKRYNFETTG
                     TITESDGSTSQFTLIRDGSSVTLTFTITKTDAALSSTGSFVFDLQNKTGGGTITNGDI
                     TMVYVFTGMCEGYYYPENHENLKQDIEGCMFSLLKL"
     gene            79743..81275
                     /locus_tag="PK937_00340"
     CDS             79743..81275
                     /locus_tag="PK937_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH31052.1"
                     /translation="MQNDLEVYIQARYPLIYLVSWEEERILKTLDQIAIHLRKMLFVW
                     TQTQGLYNYVLPDHLDESKSDPEVLLNHIAASQDNGIFVLFDFHAFIENHRIRRQLRD
                     LSKLLRKSNKTIIIVAPQMTIPLELSKDIAVMDFDLPGLTELNIAFQNALKVLHKRRD
                     VVLELTPQISEKLLKAAQGLTLVEFENVLAKSVVHSKAINAHTIQEVLEEKKQIIRKS
                     STLEYFSPDENFEQVGGLENLKQWLTKRAEAFTERAAQFGLPTPKGLLLVGTQGCGKS
                     LTAKAVAAHWNLPLLKLDVGSVFSGIVGSSEANMRHAIKVAESISPCILWIDELEKGF
                     SGMASSNFSDGGTAARVFGSFTTWLQEKKKPVFVIATSNDITLLPPEILRKGRFDEIF
                     FVDLPEQVEREAIFTIHLQKRHRTTDSFDVVKLALLSEGYSGAEIEQAIIAGLYDAFE
                     AGRELTTDDISNNLRATVPLSRVMAEQISALRQWAEKRARKASRTRTAGTGIDLDRFS
                     VN"
     gene            81303..81746
                     /locus_tag="PK937_00345"
     CDS             81303..81746
                     /locus_tag="PK937_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH31053.1"
                     /translation="MIIETLSEFFERDLLKLKEEIASYSNETAIWKIEGDIRNTAGNL
                     ALHLVGNINHFIGAVLGKTGYIREREREFSDSDVPRIKLIQDIDTTIAMAKTTFSRMD
                     DSVLYQNYPIEKHGKTVTVEHMLLHLLAHLNYHLGQVNYHRRLIG"
     gene            82109..82930
                     /locus_tag="PK937_00350"
     CDS             82109..82930
                     /locus_tag="PK937_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH31054.1"
                     /translation="MKEMQTGQFFGETDKIMHLDGITLTKTVYTHDYVDWHYHQNAYF
                     TFLLRGGLIETDRKKTVRCAPGDLLFHYWQESHCNIKPPEFTQGFHIEIEQSWFDNLE
                     LDRRMFEGSFQITDPAVRIAFYSMARELGCPDTASVVSIEDSLCRALVHVSKTTAFHS
                     NKPLWVTQIRDVLHARPFDTHTLLGLAMTLHIHPVHLSRAFTQYFGVPLGTYIRQIRL
                     QKSIERMYDPSLSLTNIALQCGFSDQSHFVRCFKAVTGITPLRYRRELCKINEPC"
     gene            83002..84117
                     /locus_tag="PK937_00355"
     CDS             83002..84117
                     /locus_tag="PK937_00355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH31055.1"
                     /translation="MKIFVILCMVLCTSIPSVSQVPIDRHDGIKVSDASSMHILPTTL
                     LKLDSAANQGVLKNITSILIARHGTLVFENYYNGADEATLHNTRSATKTITAILTGLA
                     IDQQFIPSEKTPVFPYFRDKKEILNPDPRKNQITIEDLLTMSSLLECDDDNQFSNGNE
                     ERMYLIEDYIQFALDLPIRGFAAWNPKPQDSPFGRSFSYCTAGVILLGGVLERSSNMR
                     VDAFAKKYLFDPLGIQQTAWQITPTGMPMTGGGLGLRSRDLLKIAQLYENKGIWNGKR
                     IISGSWIEKSTKPHAQARENTDYGYLCWLQKFGSNDKTYFGYYMAGNGGSKIAVFPEL
                     HLTVVITSTWYGTGKAHAQSEKILSDYIIPAVQLESK"
     gene            84104..84817
                     /locus_tag="PK937_00360"
     CDS             84104..84817
                     /locus_tag="PK937_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31056.1"
                     /translation="MNRNKFYLFLILFFLESCSTGFPEIPPGATHKFHATDFPDSFVL
                     LTVPNKYESKRPTPVLIALHGSGDRAEAFHDFWKKSADSLGLILVTPQGLEKMNMGFG
                     WNRNEATERYIKNVLVDVRKRIQIDSTQIYFAGFSSGGSLAAYLTLKNTIPNRGTVLF
                     GASLDTHSSLFNSGEKARFYIGHGTLEQNYPHISSVADTLAQSGHSVRFVPYPNIGHT
                     IPEPKQKELLNALRWILEK"
     gene            complement(84995..85912)
                     /locus_tag="PK937_00365"
     CDS             complement(84995..85912)
                     /locus_tag="PK937_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31057.1"
                     /translation="MNEWLKNKKFIVKALGALILVLLTVHLIISITRVSKQEDPFAYI
                     KEEPVAEQVIPEPRRLTIGVSLEQLNRYVPFDEAIDSLLTHEFDIQPGFIKSERIQLE
                     NVNYIRKVIQLPKNFPNTLFVLDMNVLSEQRDWDIFSVSEKLNRQAGASDFTMDIGRR
                     NLIYSRLEFTVNPRLRPMGKEVFFVVTGFGKNFDDLTRSFVELPESFAILVPKTESNY
                     KIIAHEAERTGKSVMTYMPWSKYIYYFDEKGGDQERLTQALYENLEKAANRAFIVCEY
                     SSATYQVLKKELPRLAKKGYIPRTYIQKW"
     gene            complement(85909..87081)
                     /locus_tag="PK937_00370"
     CDS             complement(85909..87081)
                     /locus_tag="PK937_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HNH31058.1"
                     /translation="MQICIYEDDHYVNLYPLTLTRPVFELRCGASTLAERIARYFPEA
                     EIFYQCRPLLASVYHEQYPDRRINHKISGECLFINGRVLMNDALMQQMSEHSGGEFIN
                     QGVIVGFWNSVKPVKLDAETRMIKYPWDLVKYNGECLREDFDRNKSGHTQATIYAGVH
                     LVNADAITIGEGSSIKPGVVLDAEHGPIMIGKHVQVMPNTVIEGPCYIGDHSVIKIGA
                     KIYGSTSIGPVCKVGGEVEGSIIQGYSNKQHDGFLGHSYIGEWCNLGADTNTSDLKNN
                     YGPVSVTINGAEINTGSLFVGLTMGDHSKSGINTMFNTGTVVGVCSNVFGGGFPPKVI
                     PSYAWVDSPKITLYKMDKAMQVANTVMARRKVTLGEKYNLLLQWIFEFAKDEHAKL"
     gene            complement(87100..88131)
                     /locus_tag="PK937_00375"
     CDS             complement(87100..88131)
                     /locus_tag="PK937_00375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH31059.1"
                     /translation="MNTKISIDDLFERIQLVKKINAEQPLFPSYEKYFDRKINTMSSV
                     SKLNRKLLAWDTILRYHGIDLENKKVLVVGCGAGWECVYMYAMGAKEVVGLEVMEMFV
                     KGMEKMFPILGLHPTVKPILQDIHTYSLDSYYDLILTVDAISHMYDHKLFLSSCFKLL
                     APNGSLLIIDDNNALNYRRRKEVANIWESWEKYGLDLKSNDGKTMHINSYEQDRYEYI
                     VERFPELSKEAKSLAERTANFNRQQIDNAILKYKKTGIMPNSVYRRGVMAYDSKYDMP
                     KEKQFNPYTLAHEIRSARFENAIHLPDLITTDTFFRKNVVKMLIVFKPFSYFFVSKGF
                     SVSGRKSSH"
     gene            complement(88173..88946)
                     /gene="bshB1"
                     /locus_tag="PK937_00380"
     CDS             complement(88173..88946)
                     /gene="bshB1"
                     /locus_tag="PK937_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNH31060.1"
                     /translation="MKLDALFFGAHPDDVELSAGGTVAKLVKLGYKVGICDITRGEAG
                     TRGSAEMRDKEAEDAAKILGVHVRENIELPDTEFVNDRANQLKAIHIIRKYQPEFVFC
                     QYWNDRHPDHMHASDLVRESSFYSGLSKIETSDKGKPQEKFRPAKVIYYAARYEFSRI
                     DGHIFIVDITETIDIKMKAMAAFASQFFNPTYKSDEPQTYISSPEFTDAIRNRAAHFG
                     SLIDTKYGESFLVREFPSVPDPVKFFRDGGQDVNKLGLA"
     gene            complement(89034..89678)
                     /locus_tag="PK937_00385"
     CDS             complement(89034..89678)
                     /locus_tag="PK937_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNH31061.1"
                     /translation="MNWNDALMPIIKKYKNKKHPLEYKNRYQLLVMTLLSAQDSDKHI
                     NEVGSRFFGVYPSMRELAKATEDEIASHIRDVRNFGNKAKWLAGIAKTVGSDENIPTT
                     METLTSLKGIGRKSASVIIRESGGPAEGIIVDLHVVRVAPRVGVTKEEKPEKIEKDLM
                     AVVEKKYWGDVGMAISFLGREICRPTNPKCEECPINENCSYFQNQIRSAKSKKN"
     gene            complement(89702..90874)
                     /locus_tag="PK937_00390"
     CDS             complement(89702..90874)
                     /locus_tag="PK937_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNH31062.1"
                     /translation="MPKPEKHEEKSDSYRIVKYESDEASEYNDFEIAPQTPFTTNIKG
                     RSSGFNPPNRFDTQHQDKSIDDITEEDIDPERTVPTELIEDKSRTILSKNDSPDIPFT
                     YGINPYRGCEHGCVYCYARPSHEFLGHSLGLDFETKIHVKYDAAKLLEKEFLKPSWKA
                     EPIAFSSNTDCYQPIERKLKITRACLEVFLKYRNPVRMITKNYLITRDLDIISQLSKL
                     NLFGAMISITTLDTGLARRLEPRTSRPDNRLKAIELLAKNNIPVGVLTAPIIPGLNDH
                     EIPEILRRAADSGATSAGYTVLRLPYQLRELFSDWLTRNEPNKKEKILHAIQSVRGGK
                     LNNTEFGKRMRGEGNMADHISNLFNLFYKKYGFDKKVVRGEPASFLRGGVVQESLF"
     gene            90967..93663
                     /locus_tag="PK937_00395"
     CDS             90967..93663
                     /locus_tag="PK937_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31063.1"
                     /translation="MEYLPSSKNPIHSQRRRGKFYDVSDILAQKPSTISGEDIQFFEE
                     LDLVYRTMCGILYNFVPLSGHPGGSLSSGRIVEGLLYQSMDYDFSKPDIDINDMICYT
                     AGHKAMGLYAMWALRNELIRIANPGMLAPERRQLRLEDLLGFRKNPTQGTPLFEKFKS
                     VSLDGHPSCIVPFVKTATGPSGVGVPAAFGLAFGALDMYPENPPRTHLLEGEGGMTPG
                     RVYEAIAAAASAQLRNAVLHIDWNQSTIDSDRCTAEGDKHGDYVQWDPVELMYLNDWN
                     VIFVPEGHNYHQVLTAQKIALSLQSDMPTAIVYRTVKGYTYGVTGKASHGAGYKFCSP
                     EFYNFIKPFEEQFGVMFPRFAGDAKNPVDVETFYWNTLLVIREVLEKNSFLPQEAAKR
                     LALSKDRLYSHNRTHRAHAPKLANIYDGTVNLSAMPDDIRLEPGTSTTIRGQVGKVLN
                     YLNKKSNGALVATAADLFESTSIAAVGKGFPDGMFNAKTNPGARLVPVGGICEDAMGA
                     FMSGVSSYRNHIGISASYAAFIVAMEHTAARCHSIGQQNYWERTGEPMHTWIMVNGHA
                     GPKTGEDGPTHADPQCLQLLCEDFPSGKLITLTPWSPDEVWPLMIEGIMKRPAVLAPF
                     VTRPAEAVPDRVKLGLDPAIAAAKGVYAFRRANMNAREYHGTLVLQGCGVASMFINGV
                     LEKIDKAGLNMNIFYVASTELFKLLPEKEQEAIFPEHLRTHAMGLTDFTLPTMYQWIR
                     WNEGLNRTLHPFRHGHHLSTGSAEKVLEEAGLHSEGQFKEIMKYARMIEKMNLTKPQT
                     DSVKAEEKIRSNRGRPKGYKTKVARVRTTKPAKQQIKNTRAKSNEHINRNKKAQTKIT
                     IKPGPGRPKKNKLVRLKLKSQTQKKSQTTKKIKPGPGRPRKK"
     gene            complement(93762..94307)
                     /locus_tag="PK937_00400"
     CDS             complement(93762..94307)
                     /locus_tag="PK937_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNH31064.1"
                     /translation="MKKTLRYLAFSLMLSGFVFSQDNKKPSPPAKSEQKVGSEKISIN
                     YSQPSVKSRKIWGELVPFGLTKNRDGKMIPWRAGANENTTFTVSKDVKIEGQTLKAGT
                     YGLHMIPGESEFVVIFNKSSTAWGSYSYDQNEDALRVTVKKETAKFQEVLTYGFESVT
                     DKSCTVYLHWELVKIPFKIEF"
     gene            complement(94402..95166)
                     /gene="surE"
                     /locus_tag="PK937_00405"
     CDS             complement(94402..95166)
                     /gene="surE"
                     /locus_tag="PK937_00405"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNH31065.1"
                     /translation="MKHILLTNDDGIDANGIFALYKELKKLGDVTVVAPQIERSAVGH
                     AITIHDPIRVLDYHRPEKFKGYAISGTPADCVKIGVKNLVHKKPDLIVSGINHGSNTA
                     TNVLYSGTVSAATEGIIMGIPSIASSMIAFDHKTDLTFAAEITAKIARIVLDKGLPSD
                     TLLNINIPPGPRTSIKGIAVTRQGRGRYEEEFEQRTDLSGRAYYWLTGKKMILDHEDD
                     VDDVVVLKNKVSVTPIQIDLTAHKFLDELKTWNIEI"
     gene            complement(95190..96062)
                     /gene="accD"
                     /locus_tag="PK937_00410"
     CDS             complement(95190..96062)
                     /gene="accD"
                     /locus_tag="PK937_00410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNH31066.1"
                     /translation="MNWFQRKKDGVNESTEKRELPNVWRKCDKCGEVQYKKDVEKNYF
                     MCVKCGYHFRVFARTYIEIMLDENSFVETESNMISVDPFQFVDTKKYSDRIQAMIKKT
                     KIKDAIRTGYGTIEGRPVMVAIMDFGFIGGSMGSVVGEKISRAIDQAYKNKLPMIIVS
                     ASGGARMMEGALSLMQMAKTSAKLALLHEKGIPYISLLTDPTTGGVTASFAMLGDVII
                     AEPGALIGFAGPRVVKEATGKDLPDGFQTAEFLVEHGFVDLIVPRNELKKTLSRLFKF
                     FEPNTDTQFKPTKS"
     gene            complement(96079..96513)
                     /gene="rimI"
                     /locus_tag="PK937_00415"
     CDS             complement(96079..96513)
                     /gene="rimI"
                     /locus_tag="PK937_00415"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNH31067.1"
                     /translation="MTNEDLDTVMRIEGASFDSPWTRSNFADELKNTDIALPLVLEQN
                     KLIIGFIVLWIILDECHLANIAVTPDYRGRGLGKLMMQNIISIARQNKCVKVMLEVRK
                     SNTTAIALYQKFLFEKVGVRKNYYHDGFMNTEDALLMDLNLI"
     gene            complement(96558..97211)
                     /gene="tsaB"
                     /locus_tag="PK937_00420"
     CDS             complement(96558..97211)
                     /gene="tsaB"
                     /locus_tag="PK937_00420"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNH31068.1"
                     /translation="MKLLVLDASTSICGVALVEGNESEIKLISSIHLNINNAHSERMM
                     LLVEYIMQQSSISSKDIQAIAVTIGPGSFTGLRIGLSVAKGLAFSFKVPLVTVPTLEA
                     IALPWIDHYRRIWVALTSRKKEYYIAFFEDHNVLIKGQVWKFDELENNLLQGDLLLTD
                     DPISFSELSGIHISNSQDGLPNVFSVGKIGLTKAIAGIYADLDNVVPDYFQGFRGAS"
     gene            complement(97208..97648)
                     /gene="tsaE"
                     /locus_tag="PK937_00425"
     CDS             complement(97208..97648)
                     /gene="tsaE"
                     /locus_tag="PK937_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNH31069.1"
                     /translation="MIPDYISHSEKETFDYAEDFAEKIKPGDIIALFGYLGSGKTIFA
                     KGLCKGLGVQEPVHSPTFVIVNEYEGYKNGKALIIRHCDFYRINTEKDIAELGIEEMM
                     DQEDYVVIAEWSERVEKKLPQSSWRVYFEKRNEQERQISIRREL"
     gene            complement(97645..98373)
                     /gene="pgl"
                     /locus_tag="PK937_00430"
     CDS             complement(97645..98373)
                     /gene="pgl"
                     /locus_tag="PK937_00430"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNH31070.1"
                     /translation="MKTHTTVFTSADELAEKTAEKIISIGHQAISMHGHFSWSLAGGK
                     TPKMVYEVLAKKHKESIDWKKVHLFWGDERDVISTHPDSNFGMAKNVLMDPIGFPDAN
                     IHRIGTEEDCMLAAQQAEDDIKDFFGLSDGGFPKFDLILLGLGEDGHTASLFPGTDGI
                     TETKFLVMDNFVKKLGRHRVTFTFPTINHASNVLFLVSGKSKSSIIKQVLQGSTEDIL
                     PAQMVRPAGELYWYLDKEAASELQ"
     gene            98487..99665
                     /locus_tag="PK937_00435"
     CDS             98487..99665
                     /locus_tag="PK937_00435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH31071.1"
                     /translation="MKEVFILSAVRTPIGKYGGVFSSIKAKELGIIAAAESIMKAGLT
                     PDSVDEAIFGCARQAGIGPNISRQIAVGVGIPSQKTAYTINKACGSGLKSIINAYTSI
                     VLGDNDVVLAGGVESMSNIPYLLTQARWGYRMGNSEILDANHTDGYFCPMADKLMGAT
                     VEDLALKYNITRDEQDAFALQSQLKAESAIKTGKFNNEIIPVKVATKKGDQIITTDEH
                     PRIGATIEDFKKLKPVFKKDGTITAGSSSGVTDGASALIIAGKEFVQNNNLKPLARII
                     GYASAGVNPSEMGIAPVPAVQKLLKKSNLRLQDIDLIELNEAFAAQALACDRELHFDK
                     DKVNVHGGAIALGHPTGCTGARITTTLVHSLHDRKAKLGIATLCISGGLGLALLIEKV
                     "
     gene            complement(99735..101123)
                     /gene="dnaA"
                     /locus_tag="PK937_00440"
     CDS             complement(99735..101123)
                     /gene="dnaA"
                     /locus_tag="PK937_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNH31072.1"
                     /translation="MSAIIELDAKSVWNRCCDIIKSQIPGPSFRTWINPIEPHVIENE
                     KFVAFIPNQFFVEWLESRYNDLILNTLREVCGRSIGVTYIVKEITEPIQTVSTPSTPL
                     VLQASVNKPKETAPVVYDSFINIRYTFDNYIEGNGNKFAKAAALAVSEKPGKTSFNPL
                     VIYGGAGLGKTHLIQAIGNQVKRNFPEKKICYVTSETFTNDFVAAIRKNDVLEFSRLY
                     RSFDLLMVDDIQFFADKGKIQEEFFHTFNTLHQLGKQIILTSDRPPKELKQVEERLIS
                     RFQMGLVTDIQPPDLETRIAILQKRSEEDGVYLPPDVCEFVAMNITSNIRELEGSLIR
                     LLAYASITNTELNLDVAKAVLKDTIASKRKNLSIETIQKIVSDYYSIPVDQMLTKGRK
                     KEIAEVRQICMYLVKEILGSSLKTIGIHFGGRDHSTVIYACKMVEEWMKSNTKIKEDV
                     EIIKRKIELAAF"
     gene            101772..101915
                     /gene="rpmH"
                     /locus_tag="PK937_00445"
     CDS             101772..101915
                     /gene="rpmH"
                     /locus_tag="PK937_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNH31073.1"
                     /translation="MKRTYQPSKRKRKNKHGFRSRMKTKGGKNVLASRRAKGRKKLSV
                     SDE"
     gene            101991..102245
                     /gene="yidD"
                     /locus_tag="PK937_00450"
     CDS             101991..102245
                     /gene="yidD"
                     /locus_tag="PK937_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNH31074.1"
                     /translation="MLRRSVLYFFVSLIRIYQHTLSVYFPSSCRFYPSCSQYSIESLK
                     SHGLFKGLWLTIRRISKCHPFHEGGYDPVPQNQNTNNKCC"
     gene            102267..103991
                     /gene="yidC"
                     /locus_tag="PK937_00455"
     CDS             102267..103991
                     /gene="yidC"
                     /locus_tag="PK937_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNH31075.1"
                     /translation="MDRRTIFGLFMIALIIILYPFYMQAVAPPADPSVKTTLAVDSTS
                     TPTHPDTLLQSKTNISKESDLPKTVSLTKDTIKQSEKIIRINSEIYSAEISNLGGGSI
                     KSWKILPYKTHDGQPVDFIESSKNLNLNFVFQGKNYNTWNIPYSIINTDSVIDLITKQ
                     KNTLTFVYHFDSERQIIQNLTFYNDRYDVDIEVQFKNFGDDITNGEYQIQWLSALKST
                     ELDRNGDLSHTISYAYMGDEKEEFDIGTDPSKETTWSGVTKWVASRTKYFAVYIIPQE
                     KDGIGCKLTSTTTHENGLLTKKSNFMNIQMKFEPNVVDRYKFYIGPMDYHILKEYDDN
                     VAVTMEWGWAVFRPLTKAIFYTFNFLYDIIPNYGWVIIVFTLLVRIVLYPLTYKTFVG
                     MQKMKVVMPRQKEIQKKYKDNPSRMQQEMMKLYKEAGYNPLSGCLPMLIQMPLLFPIY
                     QVFNQSIELRLAPFFGWITDLSRPDTITILHTGLPFIGDFHVNPLPIIMTVLTFLQQK
                     IAPMTPMGDDSDPAQRFNQKFLMYGMPIMFFFLFNGFASGLVLYWTVFNVLTMAQQVM
                     MQKFVEVK"
     gene            104066..105442
                     /gene="mnmE"
                     /locus_tag="PK937_00460"
     CDS             104066..105442
                     /gene="mnmE"
                     /locus_tag="PK937_00460"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNH31076.1"
                     /translation="MNFDETIAAISTPIGTGGVCMVRVCGKEAVNLADQIFLGHSKPS
                     QHISHSLHNGYIIDPLTKEKIDHVLIGVMLGKKTYTGLDTIEIYGHGGVLNAKRILDV
                     CIKYGARHAEPGEFTKIAFLNGKMDLSQVEAVADLIHAKTESARRSSMAQFMGNLSHE
                     IDELKKQLIKLCSLLELDLDFGEENLINITKESIDAQITNVRLQIEKLLSTYNTGHLI
                     REGAKISIIGKPNVGKSSLLNAMLKRNRAIVSDIPGTTRDFIEESLDIEGIPFIFVDT
                     AGIRQSKDVVENQGIQSSMEIAKNSDLILVIFDGSEDLNEEDINILKMVEDLIWKQKH
                     VKALFIANKCDKENYNPQRTKNDFIEISAKNDIGIDKLKRSIGDLFLNNFSIESPMIT
                     RLRHKDALENSVVSLNNAKVSLHNKMSYEFISLDIKNAIRHLGEIIGEVTGDDVLNNI
                     FSNFCIGK"
     gene            105596..107512
                     /gene="mnmG"
                     /locus_tag="PK937_00465"
     CDS             105596..107512
                     /gene="mnmG"
                     /locus_tag="PK937_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNH31077.1"
                     /translation="MDVFDVVIVGGGHAGYEAAFAASRLGCSTLLLSMETTKIGTLSC
                     NPAIGGTAKGHLVKEIDSLGGEMGFITDQVGIQFRMLNKSRGPAVWSPRAQVDRAAYP
                     LKIQELLGLKKNISIRDANIVGVVVESNQIKGVITDKNEVIKGKTVILTCGTFLNGII
                     HIGLKNVRAGRIGEKPSLGITESLISLGFESSRLKTGTPPRIHKDSINFSVCEPQYGD
                     NPPEPFSYRTDSITNTQIPCHLTYTNDKTHDILKSGFDQSPMFTGRIKGLGPRYCPSI
                     EDKINRFSDKERHQIFLEPEGYDSIEYYVNGFSTSLPEEIQISAIRSVTGLENAHVLR
                     PGYAVEYDFFPPYQINLNMETKYVEGLFFAGQINGTSGYEEAAAQGMMAGINAALKVK
                     QKDPFILRRNEAYIGVLIDDLVNKSTLEPYRIFTSLAEYRLLLRQDNADMRLMEKGNA
                     FGLIDEKQMEKFIRKKDAINQIETWIEKTNLHPKKINSFLINKGSTILEEPEPLAKIL
                     KRPEVTLTDLLECEEEIFEPKIVNLLKYRDTLREVDTSIKYDGYLKRQQEQVDRFLRM
                     ENKKIPLNFDYDKITSLSKEAREKLKKVKPGNVGQASRISGVSPSDISLLLVFLERGS
                     LGKNIQDSFNDTLN"
     gene            107516..108199
                     /gene="rsmG"
                     /locus_tag="PK937_00470"
     CDS             107516..108199
                     /gene="rsmG"
                     /locus_tag="PK937_00470"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNH31078.1"
                     /translation="MQNNQINWDVFFQILENNTLKLSSIQKNQFFSFHDFLLDWNKKI
                     NLIGRQEGNIISRHFLNSALIAFFVKFKKEQKVIDIGSGGGFPAIILNIIFPETHFTL
                     VESIKKKSDFLKETSKVVGLKNLVVINERAEKIAKTKSFSSHYEFVTARAVSALDELI
                     PLTSPFLKNEGRMLFLKGKQVDIEVESVKKSNWPDKYTLTIHRFQNIPFIPSDQESAL
                     VDIKFFLDR"
     gene            108318..115439
                     /gene="sprA"
                     /locus_tag="PK937_00475"
     CDS             108318..115439
                     /gene="sprA"
                     /locus_tag="PK937_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNH31079.1"
                     /translation="MRLLIHQYDLQVGGLFLHIISKRFLVVSFLLFFLNPSKSFSQDL
                     DSISVSLQTADSILFFINEKDIEKNINKTPTSKDVRQDSVWSDLNAIKALETELISLM
                     SEINANLSITGQLDLVINQVIENEAKKIKDSRSTRSLFSLYPFQYHNTISIDPNQNYF
                     LIRGGSIDFSQTQKLLPGYKVIAMLDSSGNYVFADEFGQTTKKQRYKETTVIKQESYI
                     SNSFVYQIRSVFTKTVVTGIASKEERQSGAVRLFSASVTTNETFQKIFGGDEVTVDAT
                     GNINLNVSGASEKSSNQSSSTGKSSQFIPKFEQQQKFNLRGTIGKKVEILFDQDSERE
                     FSFENNIKITYTGFDDEILQKLEAGNIDLSLPGTQYVTQGGQNKGLFGVKSIFKFGDL
                     TLTSIASIQNGEKKVIEINPGSGNNSTSIIKKSAKDYEKGKFFFLDKRYLNRYEMYDP
                     QDGRKHTYDPADEIINIRLFRKSEGNDRNPEKRIGIVTYNNSTLTNPNLPNMTVSQIT
                     DLDSANAATMIFLEIPKEQFSCNFQLGIINLSTKLTVGEALAVYYETSQQVFGDVSSD
                     TLKLKLLWKPTPLPTDSTWDLELKNIYAFDGITGLSAEEGESVKIKFKPSSNNDITSF
                     PNIGGKDRGLLDILHVDETSQNGKSKDPDQIIDIAPSSTVLMGYGLDYDNGWIIFPRL
                     RPFDPPTTPLPGFRDEVFPALNNDPNLGEVRIPIIYNEARSSDKFKQFNDRYSIEIES
                     KKSSSAKFSLGINVLESSEVVRLDGDVLEKNIGYTIDYTSGELILIDPRAALSTANIK
                     IEYEQAQLFQVDKKSIFGTRAEYNLANYGFGSNSFIGTTALFHSQSTVNKRVQINEEP
                     FNNFVWDINTNIELEANYLTKFINYIPFVSSNQNSKINFAAEYAKLLPTPNTSNGLME
                     NDENGVAYIDDFEAIKRTYPMGSNRKSWTLASKPIEKNASKRRKLIWYQNEISRSKIS
                     NIKTNNTDRATVLTLTLQNKISDEAPTEGLWGGVMRGFSLSSAGELGASRFIEFWVKN
                     NTPEGKHAKINIEIGEISEDQNGNGGAPNREVKSALQPVIEKNQDIGLDDLSDAGEDS
                     LLSKLGITRSQYGNLSFSQKAIVDSLQIIFPWGTIALDPDDPFGDNWNNEQARFGNKD
                     IQNAEANRDIRNIKVNGYENNTFPITDGGLRIGDTEDINNNGVIDIQNVYARYSVTTD
                     THSLDNDIIVGRGEDGWILYRLPILRPDTIINSNSIDLVLPKITSARIWISSEDPESE
                     IISVQLSEIQFINSEWKLPIEVPNGVLLNPNGKIPSNNENSKIVEISSISTDEGGGYT
                     RPNGVKREKPIDQNGNNAQREVKEQSLLLRLNELPVNSKAIISKSNSQRDFRNYGRIR
                     MFVHGDKGGSIPLPISDESSDSPLKYFIRFADRFNSYYEIEQPLYAGWSEEKNSLDID
                     IRENLTSLKFQRTGNDTLTEYNIGDNKIIRIKGNPSLDNVQFIYLGVHNTSLVNAYTG
                     EIWFNELRVTDADDKSGEAVMARLSLNVGDMLTLSGSTEYRSADFRTVEQRFNPSGGN
                     QKSWSISGNLNLHKFHIERWGISLPLSFNASHNTSDPKFLPNNDILVTDAKEANNEKI
                     RSLQLRNEEIRDSLKAWRLSASMDSSIYRDSLQNDSLLKVALNFKNMIKSESFSRGFS
                     LNFSKAKNENDFWLLRWTINNITSNFNWSETETNTPTSPQSTSRDWSSNTAYNISFAR
                     KSYKPLRWLPLTKTPFIGVVFKNISETDLNYKFITSANTDFRIDSKYRSETIRDAYNN
                     PIKKPVNTTLSGSRGYGLSVSPWQSLTSSMNVKYNMDLQGLSYGQILKGVGRGLNPFD
                     GFNDFRFDSPLDTIRGIDDSLIFNRDYSSTNTFVLSYSPQSFTWLTHTINYNAATGSS
                     RNRAANSQLNKSATIQRSVQISSSFSLKSFIGSVKEPFKNIDTRQPLSATEDSRKNRR
                     DRFKNPGSDNSAKSSDKDSGNVITKGSSFFKTASLRAGSTFEKILLMINDTRFNIDFK
                     NSFNLGALENQLDPAAKWFGYMSTDNSGMLDHIFTWDLEPINQYRTPVDSVLKQQVKS
                     LTTNSKSINYGFMYGFNFGPFTLDIKYDRSETRSISTNNTQDISIRRSSLFPYLKFVP
                     FPFYEMTLRASNVGRWPIFNLLAGATRNINFSIGYLSNENITLNEIRSRVDGIIFYNN
                     DGFVDSSRTIQNIGGRRMQTVSMDKSITFPQISFDISWKGNVTQNISFSKSNTEKLNQ
                     NTTINQNSINILSNISYSKRGGFRIPIWFLKKKQLDNEVRMSSQFTFRKTQEYSQSNT
                     RSNDGFRQPKQKTSETTYWSVEPRIDYSFTRWVTGGTFYRYEYNKSLTLGKTTRVLFG
                     LNVNITIGA"
     gene            115649..117709
                     /locus_tag="PK937_00480"
     CDS             115649..117709
                     /locus_tag="PK937_00480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH31080.1"
                     /translation="MYKLSFLIALLISCNNEVTNKQNLLKIKIERKNKEIYTLLNQES
                     ETERLNAVRCLGEFQDSNSILIIEEKIKTEKSDLVYNAYLFALGQLGFLNNHNLNLRI
                     ENFLIEQISPNKDTPYQLYVEALSKVGYQNSIIKILEIAKIDTNPTLKKHINLSLARL
                     ANRNQKNTDVFTYLKESITSKNDDVRWSAAYAYMRISDTNTVKYILDYVKDSDPRVRM
                     DIVRSLGNMKISKTNEFYKTTINTLIEILHNDPDWRVRSNAASGLGNYKFDFEEIKKI
                     YYLTALEKTKDKNEHVRISAIRSIAKSFSGDIKPNNVFLKDFFDRYLTNTEKNMENAE
                     IFNALCLMFGNKLLENGDFIEYCKNVNQIKNAYVRAKIFESLSSLTSPDLTVYYKTAL
                     NDSFLLVRYQAIEGLGKINNSEARDLIVQSINTSDPTLLSIVAGKLAEDTVYRKDDRK
                     KEVLTKKIIEAFESIQKPFDTEGKIGVIDALIQINHKSSYPFIRTFIKDPENKISTYV
                     AEQLEKITGVSYKDSLPKTNTNKSLEYKKLDQIFVKKPKAKIHTSKGLIELVFHASEA
                     PISVLNFVELAEKKYFDGVYFHRVVPNFVIQTGDPTGTGWGGPGYSIVSEYNTLHYSR
                     GVVGMASAGKDTEGSQWFITHSDQTHLDGRYSIFASVENGMEVVDQIQVGDQVKKIEI
                     IWNE"
     gene            complement(117713..118876)
                     /gene="hemW"
                     /locus_tag="PK937_00485"
     CDS             complement(117713..118876)
                     /gene="hemW"
                     /locus_tag="PK937_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNH31081.1"
                     /translation="MSNKALKNKSFGIYVHIPFCERVCIYCDFFVTTSRKYRPAFIES
                     INREIHYVASQLEAPEARTIYLGGGTPSFLEDGQITSILNSIYGNFIKSENCEITIEV
                     NPNNVNSEKLNEFNRSGINRLSLGIQSLNDTELKSLYRTHNGEQAVSAFVTARKAGFK
                     NISVDVIFGLPGQTLENLQNTVTGIIEMDPEHISIYNLTIEEQTHLHKMVKAKKVVLL
                     DDEREFEMFSWLSEYLEKSGYSQYEISNFAKDGFESKHNSSYWNGLSYLGFGPSAHSF
                     DNSRRWWNVRDLSKYIAYAGIENQKIIDAEENLNINERITEFLFLNLRQRKGINKEIF
                     LDAFGISFDAMFEKALSEAKPYINNTSDIVFLNQAGMFLYNQVCLLFVKNLNE"
     gene            119102..119758
                     /locus_tag="PK937_00490"
     CDS             119102..119758
                     /locus_tag="PK937_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31082.1"
                     /translation="MKRFLGQAMMMALIAMLAVSLTSIDAMAQKDKKKKKKKKDGPPE
                     WISKPGLYEDVIVAAGVGEGFNEQKAKSQAEQNGRKKIAEALSTEVKSLTTNFMEEAS
                     TTTEEGSSGAAQEYFSEVTQSMTNQTLSGAMIEEYWPPMGEKNGGKIKFYAKVVLKKS
                     AVVDAYKKQVEEDIAKKKIKSVKGSADDALKALDNAIGKWEKTNDNGPSTSEEGGAEE
                     "
     gene            119839..120432
                     /locus_tag="PK937_00495"
     CDS             119839..120432
                     /locus_tag="PK937_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31083.1"
                     /translation="MKSVQMFLISTLLVIMSCGGGKATSKNENKIPKWVSKPGLYEDV
                     IVAAGIGEGLSEQKAKSQAEQNGRKKIAEALQTQVKSLTTNFMEEAGTTTEKGTESAA
                     QEYFQEVTQTLTNITLNGAVLEEYWPPMGEKDGNRIKFYAKMVLKKSSLVDEFKKKVA
                     DDIAQKKIKNVKASADEALKALDKAIGKWEKTSESAD"
     gene            120606..122132
                     /locus_tag="PK937_00500"
     CDS             120606..122132
                     /locus_tag="PK937_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNH31084.1"
                     /translation="MEMQSKIKFLWLVIFLCFVQTNLWSQGPAPQWVNELPMKDGTLY
                     AVGLVNRYYEKKAGEEAADSAARSELSQTLKLNIKTTVQSWSGTIGGTGFIQELEKTI
                     DQDVFESVREAQVVGRWVDPEGLTYSLVSMPLSGVSTIQNKIVAFAKENSGGKKEAKK
                     IEKLEVSLKGLKDPKSYIRSSKPDWINGLPEEDDAIYALGIAEKFYFYVNGRESAKDK
                     ARAEIAATVKTEVNAILTDWYEVNEGSASYGSQQSFMEEMSNQVSEATLSGTQIVETW
                     YDKSTKWHYALARMSLSKVVGQIQDKAKSKVKDEKALKGLSDKLGKMINRDYLKSKDG
                     LPTWINKLPKDKEAIFAVGIDNGKYFSETQGIEKAKADARTKLAKTVSVTVENITKSW
                     IEIEEKSGDTKGEPLNDYMSSLTKEATDVSLQGSQVLSVYTKRDKDGKVTYYALGRLF
                     TGSMISKLKKKASEVMNIPQTKPNETQEQKAKRILSSGREKAAAALERLNAALDKVNQ
                     "
     gene            122163..124367
                     /locus_tag="PK937_00505"
     CDS             122163..124367
                     /locus_tag="PK937_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31085.1"
                     /translation="MKRCMWMLAMALIVAALLPMDQAQAQKKKSKDDKKSKSKKGGTP
                     EWVTKPGLYEDVIVAAGIGEAMSEQAAKSEAETDARKKIAQVLQTQVQSLTTNFMEEA
                     NTTTEAGSTGAAQEYFSEITQTMTDLTLKNVIIEEYYPPQGEKNGKKIKFYAKAVLSK
                     TDFADKFKNQVSTDVASGKINGIKMSANDALARLNKVLGGWGQDEEISSATDKPTATT
                     GLGAGISKPGGIGDTPTTTAQTGSKAASYPDWKKSLPNRPSRPGYYQGLGVAPITSNQ
                     AQDEKDAESEARAQVIRAIRSEITSKVTSAMEERSSSTGGSSSGSYSESFNSVTESFS
                     SESLKNLIVEFHTDKSSKKRYAYCEISIEEVNRQFAERLKKAINVAKTYYMAAKSSEA
                     NGDYYIALTQYLEGAKEVVIAEAINKEPIEGDIDGSGKSVSVRATFDTQLKQLLGRMR
                     VEIVSGDAQKGTKNEPLAQPLVARLVYDNMGNVMSVKGAVLVSSSVAPTVVKADLSVQ
                     TDGSGNAGFTVHAVESVNASGVNKVRIGLSTSDFEAFATQLPGALEKAKTVFKDFSFS
                     ARGSSVSRIAILIFEENIGKPQSNSIIEGDIVKQLLASKYKVIDKNEVYRAISREQAK
                     ASAESGDDAAANAIKSMADVLVIGYAKAMESVGGSTNPYGGGTSNRVSAWADCSIRVV
                     DLETGKVLASSDIQREKGLSVGAAEKAGIIALQNLSKKASKEIVDNMNAALK"
     gene            124390..124998
                     /locus_tag="PK937_00510"
     CDS             124390..124998
                     /locus_tag="PK937_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31086.1"
                     /translation="MKSFIIIGCLICSLVACGEVKKKSTEKTEKTPETVQPESESPSS
                     EDKPSSLEELVYEATGASKLRTESAARSNAELNGRTALIETLTEDGVGLLEKFYLENK
                     GMFDASMDLEKYKSDIKAVFKKSTTLKGSTVAEYTQSRKGDTTYAIVEIPLMGGYEAI
                     EEQIVSVGLKNKFLKSEHVAHFKTAFKEFFLNEKKKILTTPA"
     gene            125038..127422
                     /locus_tag="PK937_00515"
     CDS             125038..127422
                     /locus_tag="PK937_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH31087.1"
                     /translation="MRKWFICLAFVMLVNTAQAQKESDFQSGVVAYSNGQYEQAIGFF
                     ESALKYYSSIKKKKAAAADCYNYLGLLYQVLNQHGKAVENFKAAVPLFIETKNKSGAA
                     SALGNWGISSYRLKLAELRQNKQSFSLEDIGSLMAYHLKAKQYHEELKDKAGVANDLN
                     NIATMYFEGEFYDEASDYLNQALKIHSEIGYKAGMAYDMANLARLYYKTDDETGALKF
                     YQRSVGILEEIGDREGAWRTYSYIGSIYESFMKQAAILKDAQKESENRKSAIVALKKS
                     VEIIESFRGNFTNKDFFDSYLKDKNPVYKRLIRLLKLDDNASEALYYIERSKAKMSND
                     VLSAGKLAFNNKEEQKQVDKVQDLQKKTEELEKKIADEKAKPADKQDKARIDSLSKSL
                     TKNQGDFNALMIDLETKYPNIYQVIKVDPIQLSDIQKQLSDDVVILEYFPADDALYIF
                     MITKDKIEAKSVPVSSTTIDSLVNKYRYYINDVTSLMKRGRFDTKLANWKQGEDSRFY
                     ERHTKPLRDIMVKLNDYLILPVWETIKDAKYKNVTVIPAASLYYVPFQCLATETQDGD
                     ISFLVEKKAVNYLTAATLMDMINKKKQNKINNVLVFGNPDGSLPGAEEEAKIIKAAYT
                     DALLYKNQDATKDKAKDLSGNTEVVHFATHGFLSSDEPQKSFLLMAPNEAKNEDDKLT
                     ISEILKMPLKEKNELVVLSACNTSMGKSATGVELISLSRAFAIAGAPTTVATLWPVDD
                     ESTKIIMVNFYDGLKKGLAKSEAMRQAQIALIRKGDYHIHPFFWAPYVMIGNPK"
     gene            127484..128161
                     /locus_tag="PK937_00520"
     CDS             127484..128161
                     /locus_tag="PK937_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31088.1"
                     /translation="MSNSKKPQTMFGFFSKKAKKTIREDDVIWQAEDIKIHNLILRLA
                     DYSHNSDGIMIIAHFPKTLEQLIVSAKKNNLVLKELYTITDADRWATGYYNDPVAILS
                     KAIFVSSSQPPMVQEEKKKKIIVAEHFPTPEKDILLMNWAINVKAESIQWYESTDNPI
                     FTRFGGDQIRILAEKLGWQQDESMQHRFITSSIGSMQEKIFRKSTGDGDAEDVMQWFK
                     SYVPSSL"
     gene            128182..128688
                     /gene="nbaC"
                     /locus_tag="PK937_00525"
                     /pseudo
     CDS             128182..128688
                     /gene="nbaC"
                     /locus_tag="PK937_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
     gene            128815..129402
                     /locus_tag="PK937_00530"
     CDS             128815..129402
                     /locus_tag="PK937_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31089.1"
                     /translation="MLLDQVLPDYHVRAQYQILIPCKRDVVWKTLMDMDLTSIQTLRL
                     LMGLRSIPAWLMGKSKPQLKKHTLHDLSDSGMHVISEVTGSEIVLGINGKFWQLSANV
                     RMDIPAERFVSFSENDLAKAAWNFFLSETNGITTVQTETRIFCSDPKMRRKFRLYWTL
                     IGPFSGLIRIALLRKLRSNTIRYEKKNGDKGYEKK"
     gene            129410..130420
                     /locus_tag="PK937_00535"
     CDS             129410..130420
                     /locus_tag="PK937_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH31090.1"
                     /translation="MKIDIHTHILPEKLPDFAKKFGYGGFVSLEHHKPSCARMIVDGK
                     FFREIESNCWDPHVRIKESAGHGINMQVLSTIPVMFSYWSKPQDGLDVSMFLNDHIAE
                     IVRQFPQRFSGLGTVPLQAPDLAIAELERCMKIGLQGVQIGSHVNEWNLNDEWLFPFF
                     QAAEKMGAAIFVHPWDMMGQDRMSKYWLPWLVGMPAETSLAICSMIFGGVFERLPNLR
                     VAFAHGGGSFPSTIGRIEHGFHCRPDLVAVDNKRNPREYLGKFYFDSLVHDARMLRYL
                     IELVGEKRIALGSDYPFPLGEEIPGSLVESMDDLTENIKERILSGTAAEWLNINLETL
                     QK"
     gene            130421..131257
                     /locus_tag="PK937_00540"
     CDS             130421..131257
                     /locus_tag="PK937_00540"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNH31091.1"
                     /translation="MMQIFINCNDAEYAIDINSPLDISIPVYFDDLQPNTYGVPAASA
                     RAYEDGKFVGDVRRGGSCNFEEYRIIPHCNGTHTECVGHISKERIAINTYFDRLLIPA
                     TLITIEPEKAVDSTETYAPDKQNADLIITAKSLFYKLHTLPREFMSALVIRTTPNVPS
                     KKSRDYMKENSPFFSLEAMDYLFEIGVEHLLVDTPSIDRLFDEGKLSAHHVFWGVPQG
                     SHDIHLEVHSRKTITEMIYVDNSIADGNYFINIQIPNFVADAAPSRILMYQAQLKNQL
                     LP"
     gene            131261..132523
                     /gene="kynU"
                     /locus_tag="PK937_00545"
     CDS             131261..132523
                     /gene="kynU"
                     /locus_tag="PK937_00545"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNH31092.1"
                     /translation="MNFENSVGFAQKLDQEDSLKEFRSRFHIPCHKDGTQSIYFCGNS
                     LGLQPKSVRSYIEQELKDWETLGVEGHFHAKNPWMPYHEFLTAKAAKIVGANEHEVAH
                     MNSLTTNLHLLMVSFYRPTKERHKILIEKTAFPSDQYAVQSQIRYHGYDPKTALVEIM
                     PRDGEEILRTDDIEEYIQSHGQEIALILLGGVNYYTGQAMDMLRITAAGHNAGCVVGF
                     DLAHAAGNIILSLHDWNVDFAAWCSYKYLNSGPGGISGIYIHENHTRNHNIPRFAGWW
                     GHDKKTRFLMKPDFIPIPTAESWQLSNAPVLLMASLRASLDIFDEASMDRLRKKSLLL
                     TGYLEYLIHDIKTDNFVLITPSDSSQRGCQISIQTKRNGKKLFEKLSEAGIIADWREP
                     DVVRVAPVPLYNSFLDVYRFAEILKKHS"
     gene            132544..133407
                     /locus_tag="PK937_00550"
     CDS             132544..133407
                     /locus_tag="PK937_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH31093.1"
                     /translation="MKNLIYAALWVIILVLSCRQPQTHQPLSAKDGVTIDYRVVGQGD
                     TTILFLHGWGINQTYWDQQIEHFRKKYKVVTLDLPGFGKSGKNREAWTIENYAEDVAH
                     AIKTLELQSVILVGHSMSGSIVVETAVRLPSNIIGVIGVDNFKDVGHVSLPEEKAGID
                     TFIHYLGVDFVGTITASAEGTLYSAQTDSTIRKRILNDVITGNSIVSKNVLNAVFSYG
                     SKEVEMLKKLPYKLFLINSDNQPTETKVLSQIVTHSCEVFTVHATSHYPMIENAEDFN
                     RQLETIISKLK"
     gene            133412..134779
                     /locus_tag="PK937_00555"
     CDS             133412..134779
                     /locus_tag="PK937_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH31094.1"
                     /translation="MGNNITIVGAGLAGSLLSVYLAKRGYTVDLYERRPDMRQVKISA
                     GRSINLALSTRGIHALKEVGLFDEISKILIPMKGRMMHGTDGAQTFVPYGKDDTEVIN
                     SVSRGELNMRLMSLAESFPGVKIHFNQRCTGMNFETGEVFFTNESNGEKHSIFSERVI
                     GTDGSASALRSDMMRLNRFNYSQSYLEHAYKELTIPPAKNGSFRLEKNALHIWPRKTF
                     MLIALPNIDASFTCTLFLSLEGPESFSTLDTPEKVTLFFKKYFSDALEQMPDLTNHFF
                     ENPTSSLMTVKSFPWSYEDKVVLLGDASHAIVPFFGQGMNCSFEDCSELNRFIGTYSG
                     DWKKIFKEFEKSRKPNSDAIADLAVENFYEMRDLVADPRFLLKKKVEHILEEKFSDFF
                     VPKYAMVTFHRIPYSVAMERGKIQDIILDEITTGIHDPHHVDIAIAEHLIKTRLTPWS
                     DVVRL"
     gene            135020..135703
                     /locus_tag="PK937_00560"
     CDS             135020..135703
                     /locus_tag="PK937_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNH31095.1"
                     /translation="MSTARLAGFLYLVVVLTGMFTLAYVPSVLIDWNNASVTYNNIKS
                     AETLFRLGIVAGIICYTAFVLLPFVLYKLLSQVNQTFAVGMVVLSTVSVPFSLVNLLY
                     KVNVLTLIANSGNLTDPELEKTQEQIMLLLNYYGNGIQIVSIFWGLWLFPFGYLVFKS
                     GFLPKILGVFLMAGCFGYLINFTGRFLYPNYASFGIGGYVSLPATIGEIGICLWLLIG
                     GIKTQTASE"
     gene            135812..136237
                     /locus_tag="PK937_00565"
     CDS             135812..136237
                     /locus_tag="PK937_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HNH31096.1"
                     /translation="MIKIKEHDINIRFKLTALWTSVTLCYLYCDYFQLYAPEKVKGLM
                     SGQNMLDSPVKLLTAAVMMAVPACMVFLNLVLSDRILKVLNIVFGSFFTLIMLLIIPA
                     ILKPWYAFYVFYAVLEIVITIFIVRMAWKWDINKPAKVN"
     gene            136514..138616
                     /gene="fusA"
                     /locus_tag="PK937_00570"
     CDS             136514..138616
                     /gene="fusA"
                     /locus_tag="PK937_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNH31097.1"
                     /translation="MKTFTPDKIRNICLASHSGTGKTTLAEAMLFVSGTKKRFGSVDE
                     GSTSSDFHPDEIERKFSINTSLMHCEWKGHKINILDTPGYADFRGEVRSAMRVADVVI
                     IPIRATAMLDVGAEIAWNYAEEHNLPVIFCVNELDKENSNFDQTFDVLMERFGDGRHI
                     VEIGFPIGEGRPDFNKIFDIISMKLLKFELDKTGNYEALDVPSEFKERLNKLHEILIE
                     DAAESEEEFMEDFFATGTLTDEEFKKGLREGIQGRKIVPVFCTDGINSVGIKPLLDFI
                     VDYCPSPADMPSATGRTNTNAQEQRPNKSDAPFSALVFKTISENHVGELSLFRVYSGT
                     VKSGDDVLNASKNVTERIGQVFLLNGKEREDAGQVGAGDIAALVKLKDTHTNNTLCDK
                     SKPIVFGEIAFPDPVITFGIVPKGKADETKISQGLHTLHEEDPAFLYTYDVETQQSTV
                     SGQGEMHLTIITRRLKERFGVEVDLVDPKIPYKETIRGAVKDAEFKHKKQSGGRGQYG
                     HVHIKLSPKQRGSGFEFIDEVVGGVVPGRFIPAVEKGVVEAMTGGVVAGYPVVDVAVT
                     LYDGSYHDVDSDELSFKIAGAQAFRKGFKEGHPIILEPIYEVEITIPEEYLGAIMGDI
                     SSRRGQILGMEADGHFQIVKAQLPLAEILKYATILRSMTSGRGMFRKKFDHYAEVPHE
                     QAAKLMKEYEERKAAGNG"
     gene            139041..141377
                     /locus_tag="PK937_00575"
     CDS             139041..141377
                     /locus_tag="PK937_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH31098.1"
                     /translation="MLRCITILWMIGYFHSGTVSSQEYGSINGFVYDAANGEAVIGAN
                     VFINGTKLGAVTNLNGYYVIPKVPAGIYLLVVQSLGYKDYTQKITVKPGDKLRVNTQI
                     HGEDIQGEEIVVTADSIPTIMKLFEEPISRIELTGTQINQIPQVAESDLLRSLQTLPG
                     ILPVSDFSSELYVRGGTPDQNLYLLDGTDVYNPEHAFGLFSTFNTDAIKQVQISKGGF
                     GAEYGGRLSSILNVTNLDGNRKEFEGSAGISLLSAKTTLQMPIGNFGSVSASIRRTYF
                     DKTLGQAIDEIPNYYFYDGNIKAFFELDSRNTLTISTYGGRDVLNFNFNENAEDKSGI
                     DYDWGNKTGSIRWTRVFNPNWFGNFWVTGSVFSSQFHLDEIVDEDNFISDITIKGNIE
                     YHQSKQFIYKLGFEQKTMHGKYQQDFPNGAVDVDAHRYLYTMYGQTNWRPNENWDIEA
                     GLRYDYFDSPRDYQFIDPRLAAKYRINETTNLKASAGRYHQYVHRIPRTFFASIWTTT
                     DQYQKASEAYHFITGFEKTFDDVYQVEVETYYKKYNSIYTLNRFLVTDMKPSYYDEQG
                     NPIFTNTNGIFNHGNGNSIGLELMLRKDVGAFTGWVGYSLARTEYNVEGKNHGQSFTP
                     RHDRTHTINAVGNIDIRNGMRVIRGLETTKSNSRWTLGFNFIYSSGQPITRPGSLYVI
                     TPTPDNGNPVIIEYPDDINNYRLPYYARLDVSLTYEKQFKSWSIAPYLQIYNIGNRKN
                     VWFIDYPYEIKEGGTIKPKVKTTSMFPMLPSIGINFTF"
     gene            141390..142301
                     /locus_tag="PK937_00580"
     CDS             141390..142301
                     /locus_tag="PK937_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNH31099.1"
                     /translation="MNKFKFLFYIPLLLITACGEGKVSIDNINYKPKIVIRGLIFPGQ
                     PVSGISITRNLPIREQQLTLDQLIIADAKVVITNVSSTVTDTLVFDPITATYYDPEHN
                     FSVQYNTIYRLNVAAQIDGKNLNASSETKVPQQGFSIIEESSRLDSLHYRERDEKDSI
                     RTFTVRFERSVNAETYLMSMNALDADTSTYVYSPVNPYSDLKPSDVLKDLDDFKYSTQ
                     WSSLFENQPGQSEMSVFWFFLSFYGRYQMVVYAADENYSDFINTYDQVQDIDGNYDEP
                     VFHIDGDGIGVFGSAIADTVYFKVLKP"
     gene            complement(142335..143333)
                     /locus_tag="PK937_00585"
     CDS             complement(142335..143333)
                     /locus_tag="PK937_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNH31100.1"
                     /translation="MISLNDRLLHEDKPRRYAVGLDLGGTNLKYGIVSDRGEVIYQNV
                     IPVFADHGRDHVLQTMVDAAYECVMFGRKESFEVEGIGVGCPGTIDSDRGISLGPTPH
                     LPDWENAPIAEYISQKMNLPVFADNDANFMAFGEAMLGAGKQYKLVVGITLGTGVGGG
                     IIINGNIFHGSSYNGAELGHTIVEAHGRPCGCGNQGCLEQYAGGKYIVKEALSAIARG
                     DKSVLSSVENITPKTIFDFAQKNDTLCLNILEQMVFYLGAGLSSIVHVLNPDIIIIGG
                     GLSEAGDAFVSRIHNEVIKRVMKPIKPKLQIVRAKLGNTAGLLGAGYYVLYKNRSK"
     gene            complement(143317..144873)
                     /locus_tag="PK937_00590"
     CDS             complement(143317..144873)
                     /locus_tag="PK937_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNH31101.1"
                     /translation="MPSSLFDLSAGFSVVDVETTGDKSSIGYVIEIGIVNVRDFKITD
                     TFHSLVKPPIPIPPFITKLTGLTDRDVSSAPVFADIAHKVEAMMEGHYFVAHHVHFDY
                     AFIKSELERLGKYFYKDRFCTVNLSRKMLPQNTKHNLGALIEYFQIPVKARHRALDDA
                     QATAEALIRFLKMPNAEEIFSKYAEQFDRRDKQLERLEHELFKLPNVRGVYMFKDTHD
                     LPLYIGKSNHIRSRILGHVREFDIPRKRRLMKYTDHFDFTPCSSELEALILESRLIKQ
                     HQPPYNIQQRARKGYLFIKVTPDPYPRVFQVAEKKADGGHYIGPFRSGKFLDHMLRKL
                     QRHYRLCPELMKERRSPKGFCFAHQLRQCSGACGEAISAEEYREQVEDAMAVLAEYIR
                     IESKENIDSFLKSRDARSPQLVEFRKALRRVRRHMDDNPDAFDEKFLLIQKEDRVGYF
                     IHNGLLSKVLHGEELENPNLLEALSTISTNGLIETPEILDERMTIQRFVRTHRHKLSI
                     IPIRQYDFAQ"
     gene            complement(144915..145448)
                     /locus_tag="PK937_00595"
     CDS             complement(144915..145448)
                     /locus_tag="PK937_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31102.1"
                     /translation="MPVNRKVFSLRFIFIFSVLLSGIWLLSGCDELMDDENDDSDCDP
                     KPLDCHETKYSHGYLNINLTINGKNNSVPIRIFRGDFDNNDLLLDTIVTVNTFSYRLP
                     TEEYYSVVARYIQNDGDTVLAVDADDIENDRESYCDGDCYELDNGSIDLKLRFPKETA
                     ESVYSTRYIVRSRQKIQ"
     gene            complement(145448..145963)
                     /locus_tag="PK937_00600"
     CDS             complement(145448..145963)
                     /locus_tag="PK937_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31103.1"
                     /translation="MSYENRAKHSAYFEMPFRLPGFGGKNYGDLALGGTYRHNINGNQ
                     FLGVFGSFYGRPYGKAVLVKAGPRFYFQAKEYRYVIAGGLDKKFWINNKLDLFIGAGI
                     GVTFIDYRGTSSGIIDGHKLEKKEGWTPVVQAGFSHKFSRFVFIRSGLQYMNMRTIPG
                     LRFYAALGGQF"
     gene            complement(146050..147105)
                     /locus_tag="PK937_00605"
     CDS             complement(146050..147105)
                     /locus_tag="PK937_00605"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNH31104.1"
                     /translation="MLKTIQELLGAEADALLKYECKGIKKDQIHIPGPDFVDRIFIHS
                     DRHPQVLRSLQSLFGTGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVKLA
                     VEGGCNAVASTYGVLGACARKYAHKIPFIVKINHNEFLSYPNKYDQIMFGSVKQAWDM
                     GAIAVGATIYFGSAESARQIQEVSEAFAYAHELGMATILWCYTRNNAFKTSEKDFHTS
                     ADLTGQANHLGVTIQADIIKQKLPTNNGGYEALKFGKTHKKVYDELTTENAIDLTRYQ
                     VANCYMGRAGLINSGGESKGASDLAEAVKTAVINKRAGGMGLISGRKAFQKPMGDGVR
                     LLNAIQDVYTTKEITIA"
     gene            147262..150168
                     /gene="uvrA"
                     /locus_tag="PK937_00610"
     CDS             147262..150168
                     /gene="uvrA"
                     /locus_tag="PK937_00610"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH31105.1"
                     /translation="MKLNDDTQLTLDYEKKPTRSESDLFKETIIVRGARVHNLKNVDL
                     EIPRNKLTVITGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLNMMEKPDVDLVD
                     GLSPAISIEQKTASRNPRSTVGTVTEIYDYLRLLYAKVGVPYCYNCGREVKKQTRDQI
                     IEAILKMKKGARIQILAPVVRGRKGHYQELFEKLRKSGFVRVRIDGEIRELEEVDKVS
                     RYQIHNIEVVIDRLELPEKADQRLIDSVEQALEIGEGVLIVVSGEGKKNSDVTYSQHL
                     ACLHCAISYDEPFPKAFSFNSPFGACPACEGLGVIREIDETLIIPDRAKTVNEGAIAP
                     YDNPKADTWFYLQMKKILEMNGADFDTPIHKLPKKAYEMLMEGTGKTKHAFYYYSGNK
                     KKNYNGTYKGVKGIIRHFFSDTSSEQIREWAEGFMSEKVCPSCLGGRLKKEALSYRIG
                     DKNIHDAVHMSVQRCADFFDKYRIDPRIQKIGEPILKEINERLGFLLKVGLDYLTLDR
                     SARTLSGGESQRIRLATQVGSQLVGVLYILDEPSIGLHQRDNEKLIQALTDLRDLGNT
                     VIVVEHDRDTMEHADFLVDLGPGAGRLGGKIVAAGAPGDFARFDSVTAKYIRNEARIE
                     VPSVRRDGNGKVLSLKNASGNNLKNVDLHIPLGKLVCVTGVSGSGKSSLINETFYRVL
                     ARHFYKAKDAPLPYKSIKGLDHIDKVIDIDQSPIGRTPRSNPATYTNLFSLIRDLYAG
                     LPEAKVRGYKVGRFSFNVTGGRCEECQGAGLKKIEMNFLPDVYVPCETCEGKRYNRET
                     LEIRYKGKSISEVLEMTVAEALDFFDAVPPIKRKIQTLHDVGLDYIHLGQQATTLSGG
                     EAQRVKLAEELSKSSTGKTLYILDEPTTGLHFEDIKMLMNVLGQLVDKGNTVVIIEHN
                     LDVIKCADWVIDIGPEGGDGGGNIVGEGTPEEIAKIKDSYTGKFLKQELRRFRN"
     gene            150255..151286
                     /gene="hprK"
                     /locus_tag="PK937_00615"
     CDS             150255..151286
                     /gene="hprK"
                     /locus_tag="PK937_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNH31106.1"
                     /translation="MEDILYRIEKDNTRVVEKMAVRRLYEDLKEKIHLELIVSEGMDI
                     RYFAQRDLHRPGLALAGFVKLFTHDKIQILGNTENYYLLSQDDSERHVALETIFEFEM
                     PCIIVTNGNKIMPEMSALCEKKKVPLMVTELSTTDTIHLISNYLDEMFAPEMTMHGSM
                     VDVYGIGMILMGKSGIGKSEIALDLVERGHRLVADDTIRISRTSGGILMARGHDTLKH
                     FLEVRGVGILDVREMFGIRAVRVQKRVEVVVDLQLWDGKEEYERLGLDDHTVEYLGVK
                     IPIVKLPIYPGKNITVIAEAIALNLLLKVYGYDAAKIFTDRLSEEIRNKNKNAKSYVT
                     NKFLDGDKE"
     gene            151302..152201
                     /locus_tag="PK937_00620"
     CDS             151302..152201
                     /locus_tag="PK937_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNH31107.1"
                     /translation="MNENKVMEIKVGITILLATVLLVMSIVWLKGITFKPNTFEVEIT
                     FNNTAGLQIGDPVTVSGMKVGKVTDINLEADSVIVSVSLSNSVILKEDVRAVITSTDF
                     FGGKKIELVTGVADNIFKSKRFRGTREPDITELTSQLKEIATDVKGTLQRVDSVLIGV
                     NNFVGDKKMINSLKNAVYNLDSTTSRVRQIVERSDVRIDSTLSRLSSSTKIFKTMLSR
                     TDAQIDSTFGNVRLITQSVMDVTQSLDSIVSKVQSGEGNLGRMIYDDQLYWRINRTTQ
                     QLDSLVGSLREKGVKVNVKLFGD"
     gene            152217..152951
                     /locus_tag="PK937_00625"
     CDS             152217..152951
                     /locus_tag="PK937_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNH31108.1"
                     /translation="MNFLLSIMLSLFAGVLIIAGGWVFSSKKNWESRYLDFFIALGAG
                     YILGVALLDMIPEAFHATPHAMYYVVLGYLIVHLFEHVLTPHFHFGEETHNHLISNVV
                     STSALIGLMVHNFFSGVAIGSGMLTSEKVGWMIFAGTVLHKLPEGFTIASIMFASHHR
                     RTYGVVGTILLALASLLGTVMIYLFEMESLPVKDVALAMSAGTFIHVATTDLIPRIND
                     SGSRRMPLVVFLGVGLFLISYMLMHP"
     gene            153045..154574
                     /locus_tag="PK937_00630"
     CDS             153045..154574
                     /locus_tag="PK937_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HNH31109.1"
                     /translation="MLKKIFSRDTLQKVLNFIERVGNLLPHPATLFGLFALTIVIVSG
                     IFSAMDISVQHPGTGEVIRPVSLLTVEGMHRILSNLVTNFTGFVPLGTVLVAMLGIGI
                     AEGSGLIGSVLRLLVISAPKRLLTFVIVFAGVMSNVAGEVGYVLLIPLAAVIFLAVGR
                     HPIAGLAAAFAGVSGGYSANLLLGTVDPLLAGLTQEAARIVDPAYHVNPACNYYFLVA
                     STFVLSAVGTWVTEKIVEPRLGVYQGEEKPEEIKPLTVEEKRGFRYAMITGLVLTAII
                     LIGLIPENGYLRDLKTGSVLKSPFMAHIVSFIFILGAALGIAYGWGTGKFKNDSDIMK
                     SMGKSMETLGGYMVLVFFAAQFVAYFNWTNLGLIIAVKLAGILKTSGLGSVPLIVSMV
                     FMTSFVNLFMGSASAKWAIMAPIFVPMFMILGYAPELTQAAYRVGDSCSNVISPMMSY
                     FALIMAFMQRYTKDAGLGSLIATMIPYTIAFLIFWTVALVIWLIFEIPLGPDARLFLQ
                     V"
     gene            complement(154743..156575)
                     /gene="glmS"
                     /locus_tag="PK937_00635"
     CDS             complement(154743..156575)
                     /gene="glmS"
                     /locus_tag="PK937_00635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNH31110.1"
                     /translation="MCGIIGYIGRRQAMPIVMNGLKRMEYRGYDSAGVCFVENQSLQI
                     RKQAGKLYELEKDLVGVNFDATVGMGHTRWATHGVPNQTNAHPHFDEDNNFAVIHNGI
                     IENYASLKIELVKKGHTFKTETDTETVIHLVEEFYKSGMDFETAVREAIYCIKGAYGF
                     VFMCRHEPEKIIVARCGSPLVLGYGNEEFLVASDASAIVAHTRQVVYLEDNEMAILSR
                     QGIEVKTLDNKVIEKQVEEITFDISQIEKAGYEHFMLKEIFEQPNTIRDAMRGRLIAE
                     EGMPRLGGLVDHIDTLKKAKKIVFIACGTSWHAALTAEYMIEEFARINVEVEYASEFR
                     YRNPIVEEGTVVFVISQSGETADTLAAMREAKRRGAAVFGICNVVGSSIARETDAGVY
                     IHAGPEIGVASTKAFTSQLTVIALITVLLARLRNMSSAEGKQIVEEMDMLPEKVKEIL
                     KASDYIREIAELYKDRRNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKHGP
                     IALIDEEMPIVFIAPQDAIYDKVVSNIEEVRARGGKVIAITTEGNTDIRSKADHIIYI
                     PKTMDFLIPILSVIPLQLLSYHMAVLRGCNVDQPRNLAKSVTVE"
     gene            156830..159058
                     /locus_tag="PK937_00640"
     CDS             156830..159058
                     /locus_tag="PK937_00640"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNH31111.1"
                     /translation="MNLFQEQGATAPIVRDKAHYDHIFEEALNSYQSYIRIPDTETLR
                     KAYEYSYLSHLGQFRKSGLPYFDHIIEVVKILIEQNMDTVTLVAAFLHDVIEDTGKTY
                     DDIAKEFGNDVAGIVDGLTKIAGFEFGSLEIKQAENFRKMLLSMIKDIRVIIIKFADR
                     IHNMRTIDSLPRKKQERIATETRDVYAPLAHRFGMARTRWELEDLVLKVLDPESYWEL
                     SRLVSEKRTEREFYIQSVAKPLIEKLKDNGVEAQIIGRPKHFFSIYNKMKKRNKPFNE
                     IYDLLAIRIMVQEKTDCYRALGFVHSTYTPVTERFKDYVAVPKINGYQSIHTTVIGPD
                     GKMVEIQIRTHEMHQIAEEGIAAHWLYKEGKNQMDQIDKQIGWIRQLIERQRDQSDPG
                     EFLEDLKIDLFQDEVFVFTPKGDLIPLPKGSTPIDFAFAVHSEVGLHCIGAKVNGKIV
                     PLHTELRSGELIEILTSEHQSPSTHWLEIVKSSKARNHIKRYFRNIEIEQSIVLGKDL
                     LEQELRLVQLRKPFADYVDDLIKMATELTFENLDMMLSSIGRGNTSAQSIAQRLAQKN
                     IAPEKPQESSSFFKRFIKRSSSESKGIAISGVDNMMIHFAKCCNPIPGDQIMGYVTRG
                     RGVHLHRNDCHNILDAVKKEPERALAAKWQVDETKNFNVQIRVVGRDRKNFLVDVAQK
                     ISSTDTNIVSAEVRTTGNETVHLFVLQVRNIAHLNMILDKIRRVPNVITASRADTSGV
                     AN"
     gene            159079..159840
                     /locus_tag="PK937_00645"
     CDS             159079..159840
                     /locus_tag="PK937_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH31112.1"
                     /translation="MKIQFLGTGTSHGVPMIGCTCAVCASENPYNKRLRTSVIVEVKE
                     KKILIDTSIDLRQQALLHRIHHVDAVLFTHHHVDHIFGLDETRSFNRLQKKRLPCYAG
                     AYTIEQMKKVFSYIFEYPDIPGGIPMIDFIPVDKPFAIEDVQIIPITVMHGKLPIYAY
                     RIGDFIYATDCSGIPETSWEYFYNAEILVLDCLRYRTHPTHFNVEQAVEAARKIGAKK
                     TYFTHISHELDHDIVNATLPENIKLAYDGMIVESN"
     gene            159851..162211
                     /locus_tag="PK937_00650"
     CDS             159851..162211
                     /locus_tag="PK937_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31113.1"
                     /translation="MRKNFFLVPFLLVLQSCAFFNTFYNARKSYKLGKESIERSSRTS
                     REAPSNTPEMKLGFFDSEPDNIPGDAKTAFDISIEKTNKVVVLHPTSGWVDDALLLLG
                     KSYYLRSGEGDYLEAKNRLEVLMTRFPASELISEAKLWYGKTLFKLQQFQEAQEILRP
                     IEALNTTEDIRAEANLILGDIALLDENLNVAKAYYAQAQKLAKDENLRKTTLYKSAYC
                     FYKDQKYSDCVVFLNKLNQNNLSLSERFEILVFRSRALKMAGQYKTAIDHLDKILGDP
                     KYKTYFARAEFEIADILRMQGRSSDAEKQFIFIVNTYPAAPVGDCYYMLGLLHDTTQT
                     RPSADFIPDHNRAMKFYTLVRNKYASSYYAPTAVARLTFFEKMNLLKDVMSVNRRLVE
                     IIDGRIKTLESGDTATFVPDEFLVQLVKVDTSVQTNLLEDKDKAETLSDKVKQDLKTI
                     NMVSTELTEKQNEINSDIFSLVELTHRDSLLNFRKKVNERLAEYHILLAEYYANELHE
                     YDSSLAYYTKVTELFKESTSEEIAYYGLARIHQIKGFPSYLSLYKEAYEKFPNGVMAK
                     TGETILGLKAANADRYDDVFEEAQMLMVSDTQWVKAADLFQTIARSDSTKHKWPSVYA
                     MGLLEEKKKNNPQKAFLYYNTLLYGAPSWPSAEKLRQKVNAYKKAWNIQNDSAGYTID
                     TTLIRWHHDISVTQIDQKADSVRTHQEIGKNSMPITTESARRDTNRLQMTDFELEEFE
                     NLLKKKSGADSTKVGKKLKPDESLKNKKQRRIKEDESDATPIIKEE"
     gene            162208..162636
                     /locus_tag="PK937_00655"
     CDS             162208..162636
                     /locus_tag="PK937_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNH31114.1"
                     /translation="MMRYTVLLLIFMLFLYACVPNPQYGTVASRTRGEIPGKEIIKKG
                     YQTGVASYYAHKFHGRKTANGEIFDMHKLTAAHRTLPFNTLVKVTNLKNNKTIQVRIN
                     DRGPFAKDRIIDLSLAAAQKIDMIGSGTAEVRLDIIKEGP"
     gene            162657..163367
                     /locus_tag="PK937_00660"
     CDS             162657..163367
                     /locus_tag="PK937_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31115.1"
                     /translation="MLGQAPFAVASLMLINFALTLIFFFRSVRYSEAGRSSSLRFTVF
                     FVVFLWVLLQAVLSYIGFYTQFSALPPRLILTGVGPAVIAVLVFLIIPSLRNIINGFR
                     LEDLILLSVVRIPVEIMLHQLFTVGLVPEDMTYTGLNWDIFSGITAPAMMWVAKKNFT
                     WSRSVLIVWHVLTLGLLINIVSIAILSAPFPFQQINFDQPNIAVFSFPFVFLPTFIVP
                     MVLWATLTGLVKIYNVKK"
     gene            163368..164066
                     /locus_tag="PK937_00665"
     CDS             163368..164066
                     /locus_tag="PK937_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HNH31116.1"
                     /translation="MMKFLSNAFIFLVFIVSCKTKSDNLLQTLIPTTTIEAKSDNKIA
                     QAAKKYVENPFSYDITMSYHVTTYKNGQNTKTAVYPMGDINPEIGVCTDVVIRSLRIA
                     GCDLQELVHRDASKHFSFYPYFIWGQGKPDPNIDHRRVPMLNAFFKRHAKTLTIATDD
                     KHINEWQAGDIIIWDLTGSGKMDHIGIVSDTRTKAGNIPLIIHNYPSPGYVTQENVLN
                     RWVIKAHYRYDGCN"
     gene            164104..>164351
                     /locus_tag="PK937_00670"
     CDS             164104..>164351
                     /locus_tag="PK937_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31117.1"
                     /translation="MFSLSCQHPGVLFDQPMPVQASANFSFPDTLLGRWSAVLPDTTY
                     NPGPPMRVVQSTTNLIISSKLMYTQAQTISEGPIIFLDT"
BASE COUNT        48820 a        34694 c        33685 g        46952 t
ORIGIN      
        1 ggtgggaata tcaaaaaact tccacacttc ttttaccggg tgacgaatcg aaacgcggct
       61 gatgatcgtc acactaaaat ccttttatga tttggactcc gaaaggtgac tgtttttata
      121 gccgtacata aaatacaccg aaagccctac agccatccat ataaaaaaga caaaccagct
      181 tttgacgggt atttcgatca tcaggtatag acaacacatc atgccgagta tcggtatgag
      241 ggaatagttt cgcacaaacg taagcaccgc catcacgacg gcgataacca taaaaataag
      301 gaacaacact tcctgatacg cttcgttgcc gatatgtgtc atcgcgctgc cgatacgttc
      361 ggagaagaaa tatataaaaa ccagcaacag aaaaggtaca atccatttgc cgttgatata
      421 aggcaaatga aaacggttat cctcctgttc ctttttcgcc ggcaaaagta atacgcctcc
      481 gcatactaag acaaatgcga acagtgttcc tatactcgtc aggtcggtaa cgatgccgcc
      541 ttcgataaaa agcgcaggaa tcgccacgat cacaccggtg ataatcgttg caaagccggg
      601 tgtatggtat tttggatgaa tctcggcaaa ttttttagga agtagcccgt cgcgactcat
      661 actcatccaa atgcgcggct ggccgatctg aaacaccaaa agaacacttg tcgccgccac
      721 gacggcgctg aacgaaataa tatacccgag tttttccatc tggatttttt caaacacata
      781 cgcgagcggg tccgatacgc cttgaaattc tttgtaattg accatacccg tgatcaccaa
      841 tgtcgtgatc acataaatga tcgtacaaat gatcagcgaa tacatcatac cgcgcggtaa
      901 atcccgtttg ggattggaac attcctccgc cgtagtggaa atcgcatcaa aaccgatata
      961 tgcaaaaaac acggcggata cgcctttgag tacgcccgta aaatcattgg gcatgaacgg
     1021 cgtccaatta tccgtattga tataaaaaac tccgactgcg atcacgacaa atagcaccaa
     1081 caatttgaga gcaaccatca gattggcact gtttttactc tctttgatac cgacataagc
     1141 caaaatcgta attaaaatga cgattataca agccggtaaa ttcatcacta ctttcagacc
     1201 tgcaaaactc ggtgccatag tccaaaccaa ctgatcggtc ggcaagttat tccgaagtgc
     1261 atcctggtaa atgttacgtg ccgtctggta gtccaccgta agccacaccg gaagatgcaa
     1321 accaagccct tccagaagat tattgaaata actactccag gaaatcgcca cgacgatatt
     1381 accgatggcg tattcaagta tcagcgccca gccgataatc catgcgataa cttcgccaaa
     1441 tgtgacgtac gaataagtat aagcgctacc ggctacggga acgcggcttg caaactccgc
     1501 ataacataaa gcggtaaagc cgcacgtcac agccgtgatc aaaaacaaaa aaatcacgcc
     1561 gggtccgccg tcatacgcgg ctgcaccgat cgtggaaaaa ataccggcgc caatcacagc
     1621 cgcgacacca agcgccgtaa gatcccgtac acccaatgtt ttggatagat gcggcgtatg
     1681 cgcgtcggac ataccctcct cggcttcgcg catgatttta tcaatagatt ttgtcctgaa
     1741 aagcgaattt gccatgcgat ttactttaca ctatgttgag aatttgagat tggattacaa
     1801 aaaagcgttg ggcattgtac atattttatc atcctatatc aagtagagat tagatcgtta
     1861 aaagtttaat ctccaattaa gtgtgcgcgt tttttttaac attaaagttt tttaactatg
     1921 acaaaacggt tgaatttagc actttagcgt catcggggtt attaaaaaca atccgcatat
     1981 gaatatgtat ttggataaat aaatgtcgga aacgattcta acgctgataa tcagtgtatc
     2041 actttttgca ttggtattta tcccttacct ttggtacacc aacaaaaaac gagtgcgttt
     2101 cgaagccaaa aaaagggaag ctatcacgct gggacacgat aaaccggtag ctcagcatcc
     2161 cttgatcgat caatcccgct gtatcggttg tgcggcctgc gtgatcgctt gccccgaaca
     2221 tgcactgggc atgattgatg gtttggcgga actcgtttac ccggccaaat gtgtcggcca
     2281 tggcgtttgt gccgaagcgt gtccggtgtc cggtattcgt atcgtacttg acccgaccaa
     2341 atccaccact gaattgcctt tgttagatga atcgtttcaa tccaacatac ctaatctttt
     2401 tttgatcggt gaattaggcg gtatggcgct tatacgaaac gccatttttc aaggccgttc
     2461 tgtgatcgaa tatatcaccg aaaacatccg aaatactaat tcttcacccg cttcgtccga
     2521 tattcatgat gtaatcattg taggcgccgg tcctgcagga ctcagtgcag gactgacggc
     2581 caaaaagaac ggattagact atgtgctcct cgaaaaagaa acccaagcgg gcggagctat
     2641 tttgagttat ccgcgtcaaa aacttgtgat gacgacaccg gtggaaattc cgctttacgg
     2701 attactcaaa cagcgtgagt tatccaaaga aaatctgctc tccatgtgga atgacattat
     2761 ccaaaaaacc gatttagccg tctgctgtga tgaaaaaata gttaatgtaa gctgtgaaaa
     2821 taaaattttt acggtgtaca catccgccgg gcatacatac cggtcccacc acgttgtctt
     2881 ggcgctcggt cgccgaggta cgcccaaaaa actacaaatt cccggagaag atttgccaaa
     2941 agtagcatac caacttcttg aaacggtacg ctacaaacat agccgttcat tggtcgtggg
     3001 ggcgggggac agcgcgatcg aagccgcgct cgctttatcc aaacaaaccg gtgccgaagt
     3061 cactctgatc aatcgcggcg gagaatttta tcgcgccaaa cctaaaaatc aagatcgtat
     3121 ccgtgacgcg gaacggaatc aaaaaataaa aatctattac caatctcatg ttttacaaat
     3181 tgatccgacg tccgtacttg ttcagacatc gaacggccag gtatcggtag aaaatgattt
     3241 tgtatttatt tttgccggtg gcgaaatgcc gacaccgttc cttaagcaaa tcggaatcga
     3301 gtttatccaa aaatccaagg tgatcgcggc atgaataaac gaatgcatcg ctggtacatc
     3361 ctttcgatga ccgttgcagg attcatcgtt gttttaagta tcggaattta cggctgggat
     3421 tattacggca caccgcttcc tttgcgacac gatcatgtac aacacggatt attgagtcct
     3481 accggttttt ggggtcacgg gatgggtttc atcggcgcat ttcttatgac attcggcgta
     3541 ctattgtatt ccttacgaaa gcgagcccga tggatggctg ctgtcgggca gatcaaacat
     3601 atacttgaac tgcatatttt tttgtgcctt ctcggacctg cattgatcgt atttcattcc
     3661 gcattcaagt ttggcggtat tatcggggta agtttttggt gtatggtgat cgtcgtcgcg
     3721 agcggcgtga tcggccgcta tctatatctt caaattccaa aaaccattac aggtagggaa
     3781 atcacaccga tcgaattgga aaaacaaatc accacactgc gtgatcgctt gcagaaagat
     3841 gacggcattg aagaatatct tttacagcgt ttagacgcgc tttccgccgc atttacagaa
     3901 acttcacgtc tctcgatcat tcgtgctttt ccggcaatga ttatacaaaa cttacgtcat
     3961 gatgcgcgcg ttcgcacctt acttcaggag tttgaaaaaa accgtaatcc gttttcgtca
     4021 gacgtccata cacgactgac catgaaagcg catctgcaac gccaactgac caatctcacg
     4081 cttacacaaa aaatgtttcg gtactggcac atgtttcatc ttccgtttgc cgtggtcatg
     4141 ttcatcatca tgatcgctca tatagtgacg gcttttttat tcggctacgg gtggattttt
     4201 acgtcatgat ccggagactt atcgtatgaa aaaacattta ccggttttgt taatagctat
     4261 actgcttttg gccatagcgg cataccaaac attagaaaca caacaaacca cttcggtaaa
     4321 gacttcatcg aataaacccc gacctactct aggattaatg aaagaccaaa accctgacaa
     4381 taattcacag gtatttgcga taaagcaact tcaagattat ttgcaaggac accctgacga
     4441 tgccgatcat tggattcagt tgggtaattt actgtttgat aacggtaatt ttgcagatgc
     4501 cgtaaaacca tacacggaag ccttgcgttt acaacctgaa aaccccgacg tccgaaccga
     4561 cctcggcgtg tgtttattta ataccggcat gaatgatgaa gccattctac agtttgaacg
     4621 tgctttacaa tatgcccctg accacatcac ggcgacattc aatctgggca tagtccacac
     4681 gcgcatggga cacactccgt ccgctgtgca atactggaga aaaacaatac gcttaggtcc
     4741 ggataccgat atgggaaaaa aagcaacgcg tgccctgacc gatgcgggat ataaaccata
     4801 aacatgaagc attttctaca tacaatgtta tcggtgatga tggcgtggtc gcttcatgca
     4861 caattttcac cgggcgacct ggcatcgtcc cacaaacaac tcgaaggcat tcaacaatgt
     4921 acgcaatgcc acgacctcgg taaaaagata gatgatgcgc gatgccttga ttgccatacc
     4981 ttgataaaaa cccgcatcgc aaacaataaa ggttttcaca gttcggctga aaccaaaaac
     5041 aaagcctgca tcgaatgcca tcatgatcac ctcggacgag actatgcgat gatccaatgg
     5101 aaaccatcca gagaaaaatt tgatcacaca caaaccggat atatcctcga cggaaaacat
     5161 caaacgaccc aatgtgaagt ttgccacaaa gaggccttca tcaaagataa tgcggtacgc
     5221 gcacgaaccg gtgacggctt actgctcaac aaaacttttt tgggtttatc gcaagattgc
     5281 cgttcatgcc actttgacga acaccgtgat caatttgacc aatgtttgaa ttgccacgat
     5341 tttaatgatt ggaaaaaatc ttcggaaaaa atttttgacc acaacaaatc aaaatatccg
     5401 ctcacgggat tgcacgccaa agtatcctgt gaaaaatgcc acacctctgt tgccgacccg
     5461 cgtcaaaaag ccgatcataa aacagatgaa acctatttca aataccggcc gcttcagttt
     5521 gccaattgta caccatgcca tgccgatccg catcaaaacc gattcggcca ggattgtcaa
     5581 aaatgccatt tgacggcttc gtggaaacaa attaaaaacg ccttatttga ccaccggcaa
     5641 acgcgttacc ctctggaggg cctacacatc acggtgcgct gcgaaaaatg ccatcaacct
     5701 gattctataa aaccagccgt ctataaaaat atgagttttt ctaactgtac cgattgccat
     5761 actgacgcgc atgtcggtca attaactcag cgatccgatc aaggccgttg tgaaacgtgt
     5821 cataatgtca acgggttccg tccgtcgcgt tttacggtca gcatgcatgc aaccgagagt
     5881 tcctatccgc ttatcggcgc tcatgaaaaa acagattgta ataaatgcca tattaaagcc
     5941 aatgcatctg aatttttacg tcgaaccggc atccgacctc gcgttgacag cacacatatc
     6001 atcctgaagt tcaaagaaaa aacatgccaa tcgtgccata cggatatcca ccgcgggcaa
     6061 tttgcagata aattaaaaag taacgattgc gacatctgtc acaaaaccac aacatggaaa
     6121 gacattgttt ttgatcatca aaaaaactct gatttcaaat taatcggaaa acacgaaaaa
     6181 acaccgtgtg aaaaatgcca tcttttggta gatgtgggca cggagataaa acgcacattg
     6241 tacaaaccga taccgacata ctgcgaacgc tgccacaacg atattcatga aggtcagttt
     6301 gccgaatata aaaccatgac gccttcgcat ccgaaaaccg attgcgctaa gtgccattct
     6361 aacgacgctt tcaagccggc acttttcgat cacgaaaagc agtctcagtt tccactaacc
     6421 ggcgctcaca tcaaagtgga atgcgtcaaa tgccatattc gcgcacggat tacgtcggac
     6481 acactaaatg tagtctacaa acccattagt accgagtgcg cggcatgcca tccggatcaa
     6541 cacgaaggag tgtttcaaca acgatgaacc gaagccttat catcgcggca gcgctactga
     6601 gtatggccgc ctatttaccg caaccggatc ccgagtatac ggcgtggact tacgcacgtc
     6661 ctgccgcgga tacaccgtat gtaaaaatac cgcataagcc gtttgacatt ccgtgcgatg
     6721 cttgtcatac cacgacgaca tggaaaatat taaaaccaaa gatcgaatac gaccacacca
     6781 aaactgggtt tccgctggtt ggcgcccatc ttcagacggc atgcagccaa tgccatggcg
     6841 gcggtgtgtt tcgttcggcc attcgcgaat gcttcgtatg ccataccgat ccgcatcagg
     6901 cacaacttgg attggagtgt gagcggtgcc acacggagta tgcgtgggcg ccttcgattt
     6961 ttaaacatga ggatcaaaat ttattcatgt tcggcgcaca caaaggttta gattgcaccg
     7021 gttgccataa aaacctcatc acctttgcaa tgcccaacgt gcaggagtgc ggcgattgcc
     7081 atatcccgct cggcgcaacc accgatcata atgcatacaa atccatcggc gactgcatgg
     7141 cctgccataa catgaacgga tggagcaatt acccgcatgc cgacgaatgg ttctctttga
     7201 ccgggcatca tcgccgaagc tgcgagacat gccacaagca gatacccaac tatacgacct
     7261 atacatgccg ggactgtcat cgttttgacc ataaaggtga aggacacgat gatgattaaa
     7321 tttttaaacc gagtcatcat tatcatatta atctcgtact tctttattgt aaaagacggc
     7381 cggtcgcaat ctctcacggg caacaaaaat ccatttattg cacgattttc ttccggatgg
     7441 aattattatt cccgcggcgc ttcgctcaat ccgcagctaa gcgagtttac acagtatgcg
     7501 atgctcaatt atcgcacgtt ggatgatagt aatcgaatta tctggcaaat acgtaccgat
     7561 atttaccaaa aagacgccgt gacacaaact cgtctgcgaa atcaaaacga gcagtttgat
     7621 caccgaacgg taattcgtca agcctacgga tcctatacat tacgcgatgg cgaagtcaaa
     7681 ctgggacgct tcgtcccgat cggaactcat accgacgctt atccgatcaa cggtctcggc
     7741 gccgaaaata cgcgcatcac ctcgcggtgg agaggttctg tgtatggcgg aaaaattttt
     7801 gatgaatacg acaatcgccc tgaaggcatc ggctatagta cgggcggaag cctgacgtat
     7861 gaccaaaccc ggtggcaagc cggagccgga ttgagcgctg aacaactacg caatacccgt
     7921 cttaccaaag cttatctttt cggtgaatat cggccttcct acacatggcg catcgtatcc
     7981 aacaatcaat acgtagtgaa caaagcgctt ctcggctatt cgcaaaacac cttgtattat
     8041 cgtttaaaca aaaatctaag cacccgtttt tttgcggaat accatgaccg gcgcgcctac
     8101 tttcctgcgc cgacggacag ccttagtatg gatcgctttt tcaacacaac acaggaattg
     8161 atgatcggag gctctgtgcg ttaccggata ttccagtggc gcaaaatcgg gatgttagaa
     8221 acaatgcctt cgttgaaaaa gcgtctcggt aatgacaatc tgacttatgc ggcgcttcag
     8281 tttttttatc aaaattattt tttctggcga ttcaacctga ataccggatt cagctatacg
     8341 gataatcagt ggattcgtaa ttatcgctcc catctttcac ttaataagga tttttttgct
     8401 tcacgtttgg acgcttcatt ctctcttatc gttaatactt atacgtggaa ctccacatcc
     8461 tcgcgcgcca aacttttgac caccgtttcg gccgacgcca cctatcgttt tgacgcatcg
     8521 tggtatatat ccgcaggaat ttatgaagaa gtgggtaacg ccaccgatcc gcattccggc
     8581 gttaatatcc gcatgtttta ttatctgcat tgacctcggg ctttatatta tcttggtaac
     8641 aaagcgatag tttgggcata ataaacgatt tgtatttttt caaaaaaaca aatctactat
     8701 ggccgttgcc gatttaaccc gcaaaactat actccaaggc gatatcgcac aatccggcat
     8761 gacgagcata ctttcgctca tcaagttatc gcaaaaatca ggcacactcc gcctcaagga
     8821 cggcgaaaaa aacgcctcga tccggatcga aaaaaattat ttcatcgaag cctccgcacc
     8881 ggaagaaatc ctgctagggg aatttcttgt gcgcgaaggc tatctgaccg aacaagattt
     8941 agctaaaacc ctgccgttac aaaagcgcat gcacatcccg ttgggccaat tgctccaagg
     9001 tatgggatat tttggattgg acgcaaatgc gttgacccgg atcctcaatc ggtatttacg
     9061 agaagtgatt tttagaatta tgaaatggaa tagaggcaca ttcactgtgg aagaaaccgt
     9121 aaaaaaagat ttccctgtgc gagaagaaat cccggtgcgc cttaatatgg attttgtttt
     9181 attggatacc acccaacgac tggatgaatg gaaacggctt gccagcagtg ttacctcgtt
     9241 gcaatcagca ctaaaaatca accgccgggc catctctgaa aacgcatcgc tcacattaaa
     9301 cgccgatcaa ctcaccgtgc tatcgtatgt caacgggcgg cgaacgcttg tgcgcattct
     9361 ggaaaaaatc ggccacaacg aaatgttcta cctttccgta atagacgatc ttctcaaacg
     9421 ccgtatactg atcgaacgcc aggccgaagc gatgcgcgtt atcataccgg gacgcatcaa
     9481 agccgatgcg gtcaatcgaa accgccagtt tcccggcaaa tttgcagcta accttttata
     9541 taaagaaatc gacggcaaaa agaaccttca tgagttagcc gatagccttc atttcgaact
     9601 cccggatcta tgggaaaata tttccctact gctcaaaaac gaggccgtcg aaatcatcga
     9661 aggccgccga gagtttcaca atctaagcga agagatgtaa aaccaacaat tcttactaca
     9721 gaatacttta gtccggtatt attaaatttg tgttaccaaa tcattacact ccaggttttt
     9781 aatactcttg ccaacttcgt gataacattt cgctaatttt taagctcaaa caataacact
     9841 tcgataattc tctcctaata cacttccttt atctttcggc gatttcgaaa agcatttaac
     9901 ctaaccatcg ttaaggaagt cgtatgaaaa accgtttacc gcgttttcct ctttatcttg
     9961 ccgctgtttt attttttgtc attccgacat ttttgcaggc gcaagtggtc ggcaaaatca
    10021 ccggaactat cgttgatgca tccaatggtg atccgctgat tggcgcaaac gtatttgtgc
    10081 ttggcacaaa actgggcggc atcaccgatc tcgacggtaa ttttatcatc ggtaaaattc
    10141 ccgtcggtac atacagtatt gaaatttcat gcctctccta caaaaagaaa atcgtccata
    10201 atcttaccgt caatgacggc cttgcttcta cgctcaaaat ttcgctggaa ccggaggcat
    10261 tggaaggaga agaagttgtc gttgaagccg aagttctcaa aactcaggaa tcgggtcttt
    10321 tgctggcgca gaaaaaatct tccaaagtga tggacggcgt cagttctgaa caaatcgcca
    10381 aaacgtcgga cggcaatgcc ggtgcggcgc tcaaacgtgt taccggcgtg acgatcgtcg
    10441 gcgataaaga cgtctatgtt cgcggcctcg gaaaccgcta tagcaatgtc cagctcaatg
    10501 gctccgtgct gccgtccacc aatcccaatc agaaagaaac accccttaat attttttcat
    10561 ccaacgtgat agacaatatc atcgttcaaa aaacctacac gccggatcaa ccgggcgaat
    10621 tttccggcgg ttcggtgcaa atcgaaacca aagactttcc ggaatcccgt acgatcaaaa
    10681 tcggcgtatc aagtatgttc aacagcaata gcatcggtaa tgactatttg aattataacg
    10741 gcggcggttc ggattttctg gggtttgacg acggcacacg aaaactccct acggccgcaa
    10801 aacaagggcg tatcggtaca tcccaaggtg ttacggccat ccgccagttc caaaattcct
    10861 ggtcaccacg ttcttcacgt accacgccgg gtcaaagcta cagtatatcg tatggcgatc
    10921 agttcgccgt cgggtcgcgt gcgctcggat ttatcgccgc gatcaactac agctatcaaa
    10981 atcaggtgcg caacgaagta ctgcgcaatc ttcagcaagc acaatccctc gcttccgact
    11041 atgatgcctc ccgaggcatg accagcactc tgctcagcgg tatgctcaat gtatcctaca
    11101 aaataaatcc gacatccaaa gtcagcctgc gcaatctgta caccaacgta tccgacaata
    11161 ctacaagccg tgtcgaagga tattactaca attcaggcgg tgattaccgg caaacccaat
    11221 tacgcttttc acaacgatcc atcttcgctt ccaatctcca atacgatacc tacatcaaag
    11281 aatggatgga ttccaaaatc ctgatcgaag cgaccatggc cgcagccaat gcttacgaac
    11341 ccgatacgcg caatacgcac tacgcatata atagcacact ccatcaatac gagatcgtat
    11401 tggatcaacg caccaatacc cacttcttct ccgaccaaaa agaccgcgac tataatgtca
    11461 aactcaattg ggatttgaaa cttctgccga cattacgact gaaaaccggc ggtttgttct
    11521 atgataaaca gcgtgatttc gaagcccgcc gctttgctat agccggtgat ccgcagtccg
    11581 tatatcccgc tgaactcaaa acgaccaacc cggaagaagc attgaatccg gatttgtttt
    11641 ctaatggcgg attaatcttc tttgaaacga cgcgcaatac ggactcctac cgtggtgatc
    11701 aaaacatcta tgcaggttac ctttccgcag atattaccat cggaaaccgt tggaacctga
    11761 ttgccggcgc tcgcgccgaa caatctattc agcgcattga taaaaacgaa gtgctcaaca
    11821 cggcagacct ccttccggcg gtcaacctga ccttcaaagt caacgatcgt gctaacctcc
    11881 gcgccgcttt ctcggccacg ctcgcgcgtc cggagtttcg cgaactgtcc aacttcttct
    11941 attcggactt tgtcggagcg cgcacggtgt atggtaaccc tgatttgaaa cgaaccaaaa
    12001 tcaaaaacta tgacctgcgc tgggaaacgt atcccggaat cggcgaatat ttcgccgtga
    12061 gcgggtttta taaacacttt acgaatccga tcgaaatctt tcaccgcctc agcaccaatc
    12121 cggaagtgta ctacggtaat atcgctgaag ccgaactgtg gggtgtcgaa ctcgaagcac
    12181 gtaaggcgct gaccgaagcg ttgcgtgtaa cggctaacct tgccgtgatg acctcttcag
    12241 tcaattacgg aaaagccgtt tattcgcaag ccaatcgcaa ccgcccgatg tacggccagt
    12301 cgccgtatac gatcaacctc aatggttttt atgtactccg atcagctacg gaattcagcc
    12361 tggcctataa ccgttttggc aaacgcctca gctccgtagg caatgtcagc cagggcgaag
    12421 atgaatacga aatgccgttt gataaattgg atttcacggc cacccaaaaa tttggaaaat
    12481 ggacgtataa actgtccata caaaacttac tcaacgagcg cgtcatcaat cgtcagggcg
    12541 atatcattac acgtgactat gcgcccggtg tgacctatag cgtcggcgcc tcttatgact
    12601 tttgatcgca gatgttataa aggtaatgtt cgcataatcc cgacgtaaca tgttcgtgtt
    12661 agattgtgtt ataccaaaat aactacataa caagcgtaag gagttttcat gaaaaagagt
    12721 ttgattttgt tactgcttat cggttcgatc caactgattt catgcggtga cgatgacaaa
    12781 aaggatacac cggccgaagt gccctcggcg tacacctctc tgcccggcac attgatcgtt
    12841 gaggatgaaa catggagcgg caccaaaaca ttgaccggtg catattatat attacccggc
    12901 gtgacgctga ctatcgaacc cggtacggtg attaagttta cataccataa caacaatccc
    12961 gacagtgtcg gcgccatctt tgcacttaaa gccgatccta ccaatttttc cacgccgcgc
    13021 gctagcgcac gtcttgaagc cgaaggaacg gcagccaatc cgattatttt tacatctgcc
    13081 aaaacgacta aagccgccgg cgactggggc ggtatcgtac tgatcggtga agcacctaat
    13141 aatattccgg gcggtatcgg cgatgtcgaa ggcgtttcgt ctgaaatcca atacggcggc
    13201 agcaatgcgg ccgataacag cggcacactc aaatatgtac gtatcgaata ttgcggattc
    13261 ggattgatca cggactccga gctcaacgga ttgagtttgt attcgtgcgg cagcggtacg
    13321 acgttagatc acgttcaggt ttacaaatgc accgacgatg gttttgaatt cttcggcggc
    13381 agcgtcaatg caaaatattt agtctcggct tataatgacg atgacagttt tgatcacgat
    13441 cagggttgga acggtaaagg ccaattctgg cttggcgtac aaaccaccgg tgcggacaat
    13501 ggctttgaat ccgacgggcg ccatacgctc ggctccggca atccgaccaa accgacgatc
    13561 tataatgtga cattatacgg actcggaacc ggcaaagacg ccgcagatgg aaatcgtgga
    13621 atgcgtttgc gtgaggattt tgaaggtacg ctcaaaaatt tcttaatcac caatttcgcc
    13681 ggcgtcgcat ggcgtcttga gagcgcgagc ggcgactcga cctcacgtaa ctatgacaaa
    13741 ggccttctca cgatcaccaa tgccgtcgtg tataataacg accgtgatgt gaccgtcgcc
    13801 ggcaactacg gcggattcaa cagcctcaac gattcgactc gctttttcgc cgcggctaat
    13861 gttaccgaag ccaatcccaa tttcaccaac gtaggctcca aagatttctc cgtgcaagcc
    13921 ggttcgccat gcctcagcaa tgccgccacg ccaccgagcg atggattctt tgatgtgagc
    13981 gcgacctacc gcggtgcgat gggttctacc aattgggcca caggtagctg ggtgcgttgg
    14041 tcggactaaa aacgatgatc tgaaaatcct tctccatggt taggttggag atacatgcag
    14101 tcccgcgttt gttcttacag gcgcgggatt tttattgtga ataaacatca ccttttttat
    14161 gatgacatta tcacgacggg tatttatctt ttcaagccca agtcacagca tcaggagaca
    14221 cggttatgaa tttttctctg gctcaatcca tcgaaatcct cgaacgtacc ccgatgattc
    14281 tgacagcatg gcttgacgga ttatcaccgg aatggacttc gtccaatgag ggtcccgata
    14341 catggtcagc ttacgacatc gtcgggcacc tgattcacgg agaaaaaacc gactggatca
    14401 cacgtgcgga aatcatttta tccgatttac ccgataaaac attcaaaccg tttgaccgtt
    14461 ttgcacaatt tgaagacagc aaagataaaa ccctccgaca actgttaacc gcattcaccg
    14521 agctgcgcgc gaaaaatatt aaaaaactcc aatcgctgaa tatttcagaa caagatctga
    14581 gtcgcaccgg tcttcatccc gcgtttggca cggtcacctt ggtgcagctc ctttcgacgt
    14641 gggcggtgca cgatctcgat cacatcagcc agattgcacg cgttttggcc aaacgctaca
    14701 aagacgatac cgggccatgg gttgcctatc tcaaaatcct gcggcaatag tcacccgata
    14761 atgcgcagca tatttaatgt atttttttaa ataaccgttt tattcggagg aaaaacccat
    14821 gaacgtcatc attcgaaatg tttttgctgt gattgtcgga gcgatcgtcg gcagtattgt
    14881 caatatgagt atcattatga ttggcggcca gttgattcct ccgcctgcgg gtgccgatgt
    14941 gacgacgatg gaaggactca aatcatctat gcatttgttt gaactgcggc actttcttat
    15001 gccgttttta gcgcatgcac tgggaacgct tggcggtgcc accgtagccg ctttgatagc
    15061 ggccaaccat aagatgaagt ttgccttgag cataggtttt gtttttctgc tcggcggtct
    15121 gattaatgtg tttatgcttc cttctccggt atggtttacc gtcgtggact tggctggtgc
    15181 ctatttgccg atggcttatc tcggcggcaa atggatgtca aaaaatcaaa atatttgatc
    15241 ttcacaggat acgttgaaat atttcttctc agacaagatc aaatcgttta atgggatacg
    15301 ataacctttc aaaggtaacc ggggtgtgaa gcagtatatc cctaacggtt atgttttttt
    15361 tctacggatt tatgtccttt caaaacgaaa taaaacctta accggatgag aatcctccct
    15421 atttgatttt ttccctccca ttttctattt tcccccgcta aaattatttt ttcaaacatt
    15481 ttatgaggtg aatttttatg tcttcgcaga cactcgaatt taaaacggag gttaaagaat
    15541 tacttcattt gatgattcac tccttgtact cgcacaagga aatatttctc cgtgaactta
    15601 tttctaacgc ctccgatgcg attgataaag tgcgctacct ttccctcacc gacagccaaa
    15661 tatcgggcgg tgatgccgat tggaaaatca aactcaaaat tgacaaagaa gccggcacac
    15721 tgaccatttc cgataacggt atcggtatga ccgaagaaga aataatccgg tctctcggca
    15781 cgatcgcaca ttccggcacg cgcgacttca tccaacgcct tacggaaaaa caggtcaaag
    15841 ataaccctga attgatcggc caattcggtg tcggttttta ttcttcattt atggtcgctg
    15901 atcgtgtagt cgtcatttca cgcaaagccg gagaaccgca ggataaagct attcgatggg
    15961 aatccaaagc ggacggtgtg tttacggtgg aatcgtttga aaaagcaacc cgcggcacgg
    16021 acgtgattct ttttatgaac gaagagggta aaaagtacct ggaagagcgg gaaatacgca
    16081 gtgtcgttcg taaatattcc gactacatcg aacatccgat ttgtatggat gtcgaaaaag
    16141 ataaaaaaat tgaagaagaa acactcaatt cgcgtaaagc catttggctc aaagataaaa
    16201 gcgaaatcac cacggaagaa tacaacgatt tttacaaaca cgtctcgcat gatttcggcg
    16261 aaccactgaa aaccatccat tttcgcgccg agggtacatc ggaattttca gcgctgcttt
    16321 ttattcccaa aaaagcaccg tttgatctgt tttataaaga attcaaaatc ggaccgatgc
    16381 tgtatgtcaa acgcgttcag atcatggatc attgcgaaca ggccattcct acgtatcttc
    16441 gttttgtcaa aggcgttgtt gagtcatcgg atctgccgct caatgtctcg cgcgaaatcc
    16501 tgcaagccaa caaacaaatg gaagtcatca agaaaaacat tgccaaaaaa attctcgatg
    16561 ccctttccga catgaaaacg cacgagtttg aaaagtatgt ggagttttac aacgaattta
    16621 atcgtgctct caaagaggga ctgtattccg atcatcagcg caaagaatcc ttacgcgatc
    16681 tcatgctgtt cgaatccaca cgcaccgaaa agggtaaata tacctcgctc aaaacttacg
    16741 tagatggaat gaaatccgat caggaattta tctactacat gacaggcgct tcgcgtgaag
    16801 agctcgaacg ctcaccgtat ttggaagcgg tcaaagataa ggattacgaa gtgctctttt
    16861 tgaccgatga gattgacgaa tttatcgtat ccgaactcga atacagcggt aaaaaactca
    16921 aatctttgac caaaggcgac atcgaactcg acaaagataa aaaagaatcc aaaaaagaag
    16981 ccgaaaagaa atacggtaag ctgattgacc tgctcaaaga tcagttgaag gaagagatca
    17041 aagatgtgcg ggtctcggca cgactcaaag attcggcttg ctgccttgtt gccgacgaag
    17101 acggcatgga ccaaaacatg gaacgaatca tgaaagccct gggtcaaaat gttccgtccg
    17161 gcaaacgtac gctcgaaatc aatccggacc atcctctgat cggtaaaatg cagatgatgt
    17221 ttgatatcga taaagatcaa ccgttgctca aagactatac ggcgctgttg tatgatcagg
    17281 cgctcgtact ggaaggcagc aaacccaaag atccggtgct ttttgccaaa cgtatcgctg
    17341 aaataatggt aaaagcataa aaaggatttg caaaacaaaa atggatcggt tataattctg
    17401 cgctatgata aagaacaaca acatacgtta ttaccgttca aacggactta tccttcgtcg
    17461 tcatacacgt atggatcaag tcaatttgat atattggagc gcatgagccg attttaattt
    17521 tttgaatatt ttttctaacc ccggctccgt gaacagccgg ggttttttat tttactaaag
    17581 gagtattatg atctcgggga aagatctcat cgctatgggt tacaaacccg gcaaatggtt
    17641 caaagatgcc attgaacaca tcaatgaaaa tcatctaacg gatgaaaagt tggaaaatta
    17701 tttacagcag ataagtccaa aggaagtgtt gccgctcgat aaagctattc catacttcag
    17761 caatattcgc gctgaaaatg aggtagagct tgaaaacatc aaaagcgttg acaccacaat
    17821 gatggagctg atgaaaacac cgacgatgat caaaggcgct atcatgcccg atgcatgtcc
    17881 gacgggtgaa aaaggccaaa taccggtcgg cggcgtagcc gtcgcccaaa acgctattca
    17941 ccccgctatg cacagtgccg atatttgttg ctcggtaatg atgaccgggt ttggcaaaat
    18001 agctcctaaa tcggtgttag atgcggcaca tgcgataacc catttcggtc cgggtggtcg
    18061 tgacgaattt tcaaatctcc cgagcgaatt ggtcgaacaa atctgttcca atccgtttct
    18121 gaacaatgaa cggagcaaaa cattggctgc aacgcatttg ggtacgcaag gtgacggcaa
    18181 ccattttttg tttgtcgggg tatcggccaa aagcggcgaa acgatcatgg tcacccacca
    18241 tggtagccga gggttcggcg cacaactgta tgcacaggga atgaaaatgg cggagttttt
    18301 cagaaaggag atctcaccca aaacacgcgc ctgcaatgca tggattcctt ttgatacggg
    18361 cgaaggcaat aactattggg aagccttaca aatcgtgcgc gtatggacca aacttaacca
    18421 ccgtgttatt cacgatgctg ttttgcattc gctgcatgca gatattacgg agcgtttttg
    18481 gaacgaacat aatttcgtct tccgtgaaaa agacttgttt tatcatgcga aaggcgctac
    18541 gccacttgat gataagtttg ttcccgattc cacggatggt ttgcgcttgg taccgctcaa
    18601 tatgagccaa cccgtactcg tcgtcaaagg cgcgaccaca aaaaataatc tgggttttgc
    18661 gccgcatggc gccggccgta atatcagccg gggccaacac aaacggttgt atgcgcataa
    18721 aactaaggag cagttgtttg ctgaagaaac acgcggtttg gatatccgct ttttcagtcg
    18781 tcacattgat gtatccgaat tgcccagcgc atacaaaaat gcagatgctg tcaaagcgca
    18841 gatgaaagaa ttcggtctag gcgaaatcct ggacgagatt attccctacg gctgtatcat
    18901 ggccggcgat tgggaaaaaa atgcgccgtg gcgaaagaag aaaaacaaat agcattagat
    18961 ggacaacacc ggaaaccgct aaaggttatt ccggtgtttg ttttatgggt atcatcgtat
    19021 catgtttaaa agagatgctt cgtacccgta tgcattgtta ggttcattgg cagacacgac
    19081 aattatctta ttctgaaatc ttgttttttc tctcattatt gaaacaatta agtagcaact
    19141 tacgtaaagg tcttttactt ttccgttacg attccttagt tgtttttttc ttatctattt
    19201 aagatatata accttacgac ctgtttattt agtggctgtg atttttgatt atttagatta
    19261 aaaaatctga ttttattgaa tttgagatat catattattt gaaaatactt ggccgtatat
    19321 gtcttaaata tggcgagcgc gtaatcctgt tttgtgatat ttttaaaaaa ctatagaaat
    19381 gtcatcccgt cccatacgtt acttttaatg gagttttctg ttatgaaaaa agtcgtgttt
    19441 tattggcttg ttaccgtttt gtgcctgact tcgtttgctg ccgcacaata cgacagttca
    19501 ttattcagcg gcatgaaagc ccggagtatc ggtccggccg gcatgagcgg acgtatcgct
    19561 tcgattgacg ccgtcaaaag cgatcctaac atcatctatg tcggagccgc taccggcggt
    19621 gtctggaaat ccattaacgg cgggattacg tggaactcta ttttcgacga tcaatccacc
    19681 ggctccattg gttcggttgc gattttccaa aaaaacccaa acatcgtttg ggttggtacc
    19741 ggcgaatcca atgtccgaaa ctctgtcggt gtcggtcgcg gtattttcaa atccatagat
    19801 gccggcaaaa catggcaatt cttcggtctt gaaaaagccg aaaaaattgc acgcgttatt
    19861 ctacatcctg aaaacgccga tatcgcgtgg gtagccgcca tgggaactac ttggggcgaa
    19921 aacaaagaac gcggcgtttt taaaacaacc gacggcggta aaacatggaa acaagtactc
    19981 tatgtggacg aaaaaaccgg cgccgcggaa ctggtgatag atccctctaa tcccaataaa
    20041 cttttgtgcg gtatgtggga acatcgccgc tacccttggt atttcaaatc gggcggcaca
    20101 agcagcggtt tgtatatatc tacggatggc ggtgaaacat ggaaaaaaca aaccaccaaa
    20161 gagggtttgc ctgagggcga actgggtcgc atcgcattgt ctttttcgcg caacaaaacc
    20221 tccacagtgt acgctctggt cgaagcttcc gaaagtgtgt tacttcgctc cgatgatggc
    20281 ggctacaaat ggcgaaccgt caacaaagaa aaaaatgttc atcctcgccc gttttatttc
    20341 agttccatag aagtcaatcc cgtcaacgaa aatatcgttt acagaattca cgttacattt
    20401 gatgtgagcg aagacggcgg caaaagtttt tctcttctga caccgtacca aaacattcac
    20461 cccgattttc atgccttgtg gatgcatccc aatggtgaga tgatgatcgc cggcaatgac
    20521 ggcggtttgg ctttcacata cgaccgcggt aaaacatggc gctttgttca aaacctgccc
    20581 gtcggccagt tttatcatat cagctatgat atggaatatc cctataatat ctacggcgga
    20641 ttgcaagaca acggctcctg gcgcggtccg agcaccacat tcaaacggga tgccatttat
    20701 aacgacgtct gggatatggt tgcgttcggc gatggatttg atacggaacc cgatcccgaa
    20761 aatgccgatt ttggatacgc gatgtcgcaa ggcggcgctt tggtgtattt caatcacaaa
    20821 accggtttac aaaaattcat tcgcccgacg gaatctgccg tcaaacaccg gtataactgg
    20881 aatgccggat ttgcactgga tccttttgat gcgaaaacca tttattatgg aagccagttt
    20941 ttgcacaaga gtacggataa aggcgatact tgggaaatca taagccctga tctcactacc
    21001 aacgacacga cgaaaatcaa tcttggcaaa gaaaccggcg gattgacgct cgatgtatcc
    21061 ggcgctgaaa atcattgcac cattctcacc atcgcgccaa gttccgtcaa gcgcggcgtg
    21121 atctgggtcg gaaccgacga cggtaatgtc caagtcacac aagacggcgg taaaacatgg
    21181 acactggttt caaaaaacaa atccatcgca gcacccgcag gaacatgggt gcctcatatt
    21241 gaagcgtcca aatttgatgc cggcacggcg tacgtagtgt ttgacgatca tcgccggtcc
    21301 aattggtcaa cctacgtcta tggtacacgc gatttcggaa aaacatggac taatcttgcg
    21361 accaaagaag ttgacggatt cgttcactgc ttcgaagaag atccggtaaa caaagatctg
    21421 ctgtggcttg gcaccgagta cggactttac gtttctttta acagcggcaa aaattggatg
    21481 aagtggcgcg ccggtgtacc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnacgtttc ttttaacagc ggcaaaaatt ggatgaagtg gcgcgccggt gtacccaccg
    21661 tgccggtcta tgatcttgca actcatccgc gcgaacatga tctcatcatc ggtacgcacg
    21721 gacgcggcat ctatcttctc gatgatatta caccgctacg aaagtttgct gaaaactcca
    21781 aaaagaacgc gcacctgtac gaagtcaatc ccggctatca gtataattat tcgttttggt
    21841 caggatcaaa cgccggcgga ccaggcaacg ccgccttcaa aggtgaacaa cgcccgtacg
    21901 gttccatcat tacgtatttt gtgaatacat ccgatagcat caaggcttta gaagacacgc
    21961 ccaaagaacc gaagatgaaa atcgaaatat tggataaaga ttcggttata cgtgtgatca
    22021 aaggcggcat gaagcgcggt atgaatcgtg tagcttggga tttgtcccgc ggcgctttca
    22081 aaggcatatc ctccgtcgat agtaccgatc ttgaagcttc cggcatctat gtattgcccg
    22141 gtatgtatac cgtgcgtatg cggtataacg gacaggaata taaacaatcc gtcgaagtta
    22201 agaacgatcc gcgatttgct atttccaccg atggccgcaa agccatgcaa acatgggctg
    22261 aaaaggctgg tgaacttcaa accgcggccg caaccgttta tcaacaaatc gccgaatcca
    22321 aaaaatccat aaccgcgatg ctggccgtca ctggcaatct ggattctgcc aaatccaatc
    22381 cgatcaaacg tctcgcggag tccgcacaga aaaaaatgtc cggcattcag gataaaattc
    22441 agtcggagtt gtccaaacaa ggattcaatg acaactcgga tgagttatta caacagatcg
    22501 gtgaactgca gttttatatc acgtcacact acgaagcccc gacgcaacag gccaaagtga
    22561 aatacgagcg cctgcggaaa gtgctcgaag gattgatcga agatcataaa aagattcaga
    22621 ataccgatat tccggaattg caaaaattga tctccaatgc cggtgtgaat tatttcaaac
    22681 aaccgcagga aataaaactc aacaaataag atttgatctg tatttaaaaa agggaaggtt
    22741 tgtgccgcta aggactcaaa ccttcccttt tctgttttag aatagttttt ttttgtgtat
    22801 tatagcggtc atgaaaacaa aagcggcccc aacaaaaaaa ttaaccgtcc ccctcggcca
    22861 acgaagctat cccattacca tttccgataa aatcagcacg tccatcgtac cacaaaaaaa
    22921 atccgtagaa aaaaccattg tcatcaccaa tgaaacactc gcttttttgt atcaaaaaaa
    22981 attgagtcgt tttttttcgg cccgctggat agttataccg gacggtgaat cccataaaac
    23041 actttctaca ctcgaacaca ttgtgacgca gttgattgat tttcgagcgg atcgccaaac
    23101 gcagatttta gctttcggag gcggtgttat cggcgatatt gccggattgg cggccgctac
    23161 ttttatgcgc ggcattcctt acgtacaagt gcctacatcg ttattggcta tggttgacag
    23221 cgccgtcggc ggcaaagtcg ccgtcaatca tccgctgggt aaaaacatga tcggcgcatt
    23281 ttaccagccc cgatcggttc acatcgctac ggattttctt aaaacacttc ccgatcgtga
    23341 aatgcgctgc ggcttggctg aaatcattaa gtacggcatc gcgttagacg ctaagttttt
    23401 cgattggctg gaaaataatc aggatttggt gctgacacgc caaccggaag ccgtttcctt
    23461 ttgcatccat cgcgcgtgca gcataaaagc tgccgtggtg gcttccgacg aaaaagacac
    23521 cggccgacga atgattctca acttcggtca tacctttgct catgcgatcg agcgtgtgtc
    23581 cgggtatgaa ccgatcaaac acggcgaatc ggtttttttg ggtatggtgg ccgcattgtg
    23641 catttcgctg cgattaaaaa aattatccgg tgcgaatttt gatcnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnngccgca ttgtgcattt cgctgcgatt aaaaaaatta
    23821 tccggtgcga attttgatcg catgatggcc gtcctcttac cgcaagttga tgatatcttc
    23881 tcgcgttcgg aagtaaaaaa atcatggttg gctctggata aaaaagacgt tctaaacgca
    23941 ttatatcacg ataaaaaagt ttcccaaaat caaattcagt ggatcatgag caccgctata
    24001 ggaaaaactg aaatcgtatc cgggattcat aaaaaacata ccgaagaagc attcgacttt
    24061 ttaaactaca tcattcatga aacccttgca taaaataccg ctgtttccgc tcggcgtcgt
    24121 tctttttccg ggtatgccgt tgccgttgca catttttgaa gagcgttaca aagcgatgat
    24181 cgccgaatgt ttggaacagc aatcggtttt cggtgtggtg tactacaccg gagaaaattt
    24241 ttataagatc ggttgcaccg cccgtatccg cgacgtactc aaacgttttg acgacggacg
    24301 catgaatatc gtcaccgaag ggtgcgaccg gttttcgatc catcgcgtga ccaacgataa
    24361 accttacctg caaggcgaag tggagtacat tgacgacatc tcggaaaata tcgtcgaaga
    24421 aaccgaattg tcacgggcgg cggcgcaaac atttaaagac gctgtaaaat tgatcaataa
    24481 agaagatgta cccgacctca atctgctgga tgccaaacgt atctcttata tcatcgcctc
    24541 cagcgcagga tttagtttaa acgagcgcca ggcttttttg gaaatgaatt ctaccgccga
    24601 gcgccttcgc aaagccacgt ccgccttgcg ccggttgatg gagcgtcaca aaatgaccag
    24661 cgacatcgaa agcatcattt ccggcaacgg ttatttgcat aattctaaat tataatacgc
    24721 ccggagtcac ccgtgtccga tcttcacaat aacggaaaaa aaatatttcg ctcgcagcag
    24781 gccggtatcg cggcgctttt tgtaaccgta gctacgctcc tgagcacggt cctcggtctt
    24841 gtacgcgata aagtgatggc ccattatttc ggcgcaagtt tggacaccga tttgtataac
    24901 tacggcacgc gtatcccgga tgctttgcag accatattga ttatgggcgt tacgtcgtcg
    24961 tcattcatac cgatgtttag cgaatttctt gcccagagag gccatgaagc cgccaatcgt
    25021 ctggccagca gttttctcaa tattactttg accgtattta tcggcatctg tttactcatc
    25081 ggtatcggta tgccggtgat atgcgatgta tggctttcgc cggatttgcc cctcgaccaa
    25141 aaaaacacga tcgttcacat agcccgtatc tttcttgcat cgcagatcgc ttttgcatta
    25201 agcaaaatcc tgagcggtat tttacaaacg cacaaacatt ttacggcgta cggattggcg
    25261 cttttggttt acaatccggc tatcattttg ggtatggttc tttttcacga tacgatgggt
    25321 attgattcgg cggcctacgg tgcgcttctc ggttcggtat ttgtcgtttt ggtgacgctg
    25381 cttgatctgc gcggcaccgg atatcaatat aactttcagt ggtttttcga caaagaaggg
    25441 gttcgtcgta tttttgtttt ggctataccc aattttctaa acatggcgct gcttcagttt
    25501 gtcatactgg cgtattccaa aatttccatt cacatgccgg atggcagttt tagtgccttt
    25561 aattacgcgc tcaattttga aagttttccc gtaagcgttt ttggaatttc ttttgttacg
    25621 gcgatttttc cgtatttgtc ggaaaacgcc agtcagcgta attttcaaaa ttttaactac
    25681 aacattcaaa attcactccg ccaaatattg tttttgactt tgccgtcagg tatcggcatg
    25741 gctatcctaa gttaccctat catcagtctg atattgggcg gcggacgatt tggttcggaa
    25801 gcgattgaga tgaccgcttc cacgttattt ttttacgcat tagttgttcc gctggaaagt
    25861 ttatggtatc ttttcgcacg tgctttttat gcgatcaaag atacgtggag tcctttttgg
    25921 taccggttta tcggtacggt gatcaatctc ggtattagtt attcgttagc ccaatcgatg
    25981 ggaccgtcgg ctttttcact ggggctactc gcggcgtttc ttattcagat tgttcttttt
    26041 acgtttggtc tcaagcggcg cgttcccgaa gtggatatcc gaagcgcggc ggttaccggc
    26101 ggcaaactct ttgtttgtgc gctcgcgcag ggtgcggtcg tctggttgct cgtgcagtgg
    26161 ttgccgacat taagttttat ccatcaaaaa gcgcagcgta tgcaagacct tatggtggta
    26221 ggtatcagca tcttcgccgg tatgatcatt tattttaccc tgacggctgt ttttaagtgc
    26281 gctgactttt ccgtattgca aagggtgctc aatcgaatta tgaaaaaaga gagtgtttag
    26341 atcatgagtc atgttttgat cgtggacgac gaaaaaaata ttcgtcgaac cgtagagatg
    26401 attttacaag atacttatac tgtctcgaca gccgaaagcg gacgggaggc ttttaatatc
    26461 ttaaaagagc aaccgattga cctcgttttt ttagatctat taatgccgga gatgaatggg
    26521 attgacgtgc tcaaagagat acgcacgatc aatcccgata cgacagtcgt catgatgtcg
    26581 ggtcacggca ccatcgaaaa cgccgtggag gccgtcaaac tcggcgcgta tgattttatc
    26641 gaaaaaccgc tgaccaaaga aaaactcatc atcgcggcca aaaacgcgct cgagcgcgtt
    26701 tcgctcaaag aagaaaaccg aattctgcgt caggaagtga ataaacgttt tgaaatggtc
    26761 ggcgaaagcc cgggcatgaa agaaatcaaa gccacgattt ccaaaatttc cccgaccaat
    26821 gcccgcgtat tgattttagg tgaaagcggc accggtaaag aattggtcgc ccgcgccatc
    26881 catgataaca gcccgcgcaa atccaaaccg tttatcaaag tcaactgcgc tgcgattccc
    26941 gaagaactta ttgaaagcga attatttggc gccgtcaaag gcgcattcac cggcgccgta
    27001 gccgatcgcg acggcaagtt tgttttggcc gacaagggtt ctattttttt ggatgaaatc
    27061 ggcgacatga gcctcaaagt gcaggccaaa gtgcttcgtg cactgcagga gggcgaattt
    27121 gaaaaagtcg gcggtaccaa aacgatcaaa gtagatgtgc gcgttatcgc ggcaaccaat
    27181 aaagacctga aagaagaagt caaaaaagga atgttccgcg aagatcttta ttttcgtttg
    27241 aatgtcgtac cgatcttgat gccgccttta cgccaaagaa aagccgacgt cccgtttctg
    27301 atccaacatt ttgtcaaact ttgtgccgcc gaaggcggac ttccggaaaa aacattttcg
    27361 gacgaagcga tgcaaacatt atcggattac gggtggccgg gcaatatacg cgaactgcgt
    27421 aatgtcgtcg aacgcacgct gatcctcagc ctgaccgata tcgttcaacg cgaagacctc
    27481 ccgcgccaaa tattggccgc cgaaagtacc acggccgtgg cgaccaatct gaactatgaa
    27541 gacggcaaaa cgctcaaaga cttcaaagaa gaagcggaga aacaatacat catacaagta
    27601 ttgcaaaaaa ataactggaa tgtatcgcgg acggcggagg aattggatgt agaacgaacg
    27661 aatctacaca aaaagctgaa gcagtataat atagaaatgg ataaatcgga gaattgatgg
    27721 tgtttttcgt tatttataat aacggtgcat tacaaaaagt gtcgcacaaa aaaagaaaaa
    27781 cacggacacc aatagtacca taagcagcaa gtcttgatta gcagaagcca tataacgaga
    27841 tcctttgtga gggtaaatta cttatacaaa tattctaacc aagcggtcac accatgaaaa
    27901 aaatgttttt attgattgcc gggagcatgc tcctatgggc gggttgtacc tccgcacccc
    27961 gtatgcgttc gataccggaa ggcgctgtaa tacctgaagt taaagtactt attgcccgct
    28021 cctcccaatc cgtgaatatc tcttccaaag aagcttttgc cgtcaaaacg gatgacaatc
    28081 aggatgtcgg atttatttca gccggtcaga atgtggaact gcgtttcaac gaaaaccgcg
    28141 tggaaatcta tgatgcgacc ggcaaatcgt tgggtgcgta cggcaatctg atttttacga
    28201 ccaagtcgtc ctcgcagcgc tacatcatca ataacaaaca ataccgcggc agtttatggg
    28261 taacgacgca agagggtaaa atattggtca tcaaccgtct cgatatggaa tcgtacctga
    28321 tgggtgtcgt acgcaatgaa atcggaaacc tcggaatgga tcaaatcgaa gcttccaaag
    28381 cgcaagccgt cgccgccaga acctatgcga tcaaaaataa aggcaaatac aaaatttttg
    28441 atttccagag cgacatcaat gatcaggtct atgacggcgc gggtacggaa accgaagtga
    28501 gcaatcaggc agtgatcgaa accctggggg aagtggcgga atatgacagt aagccgatag
    28561 acgccgtatt ttattcgaca gccggaggcg tcactacacg ctcgcactgg gtatggaaaa
    28621 gctccgatcc gaaaccatac ctcaacagca tcagcactaa aatcggcgat aaggctttgg
    28681 atgaagcctc gccgcattat cggtgggaag tgaagtggac cggtgaagag atcgaaaaaa
    28741 tcatcaaaac caatctgccc acggttttag cggtcgtcta tcccaatgaa gattttagca
    28801 aattagccac acagcgtctt tataatctgg ctgtcgtttc gcgcgactcg tcgcagcgcg
    28861 tacaggaatt caaaatcggc ttctccaaag acagctatac cgttacgggc gagcaagcgc
    28921 gtcgcatcat gcgcggtgaa aaatatatgt tatacagctc acttttcaga ctcgatgtga
    28981 tgcgcaacgc cgacggcacc atccaggcgg tcaactgcaa gggcgccgga tttggtcacg
    29041 gtgtcggtat gtgccaatac agtgcacgcg aaatggccaa aaccggttat tcgtataaac
    29101 agattctcaa attcttttat cgcggcaccg atattcgcaa gatgtattga tggaaattct
    29161 tgatcacaaa aagaccccgc actttacagt acggggtctt tctaatttac aataactcag
    29221 gttacctttt ttctaaataa aaaaatcacc catgacgaca caagtcgaaa cttcaaaaga
    29281 cctgactcta tcatatataa tatacgtatc atgcattttt tgttagcaaa ccgaattaag
    29341 aacccatcgt atttgtctga atatagattt tagaaatgtc gctatctttt atgttaaaag
    29401 gattttaaca tcaacgtcaa ctaatcgaat cacacgctac agaaatactt attgagtctt
    29461 acaatgtagc ggcttcttta aattattttc atcaaataac agcggaggtg agattgaaaa
    29521 ttgatcgtcc tgctttattc tgtatttgga tagaaatacc cgttttcagt ataccgaacc
    29581 atatggagtt gtacaaaaag gcgactaatt agtcgttgta tcactaaatg cttaaagtta
    29641 atgaaagtat ttccagtttt cttcataaac aaaattaact ccattaacat aatacctcat
    29701 cgtgcgaact tcagaagctg catagattat tttcacggta tcaggcgaga cctttgtcac
    29761 tgttaccccc cttgcgtgat atgcaaaaaa aacgttacct gttctgtttc ccaaattttg
    29821 caaatcatca tttgtcatgc aaactgagac gttcgcatcc atatcaactg tagcaccaca
    29881 gtcgcctaga tacgaaacga ccattactcc attattaaga ttttgaactg tagttattga
    29941 actgacagca caatctcgac aactccaaat cgctattaca aatatagata atattagaac
    30001 aaaatttttc atcaaatttt atcaccttag accatttact cgtacttgaa tcataatggg
    30061 aaccgaacac gatacttact catcaatata aatataattt tcagaattca attttttgag
    30121 ccgatgaaat tcaacgtaat catttgaagt cggtgtgcaa tactctttgt atattaacaa
    30181 tggagatcgt ttgattgaga actcgacatc attagcataa gtatttacaa cacacgtatc
    30241 accattccat gaaataaaaa aaaccattac tggattcatt acatcaagtt tattaaactt
    30301 aggcataatg aatagatcgg gaattgtatc cgcttcatat tcacatagag taatgtaata
    30361 actacgatgg ctggttttaa tcattgttgc agtatattta ccgtcttttg aacgaaaaaa
    30421 aacacgctca cccccatctt tgcaagcaac ggaactgata agaaataaaa gaatgatttt
    30481 ctttaaatat ttaatacttg tgatacccat attttgacag agttaaatca cctctgaatt
    30541 ctcgctcaat ctatattcaa cttcgtgcat cctcaccgca ttttagatat aaaacacaca
    30601 tcactatcat aaatgagaat atgaacatat atcgatattt tttttcttat caaaatcatc
    30661 attttcaaac caatccatga taatagaact acctttctat tgggactctc aaaacggggt
    30721 ttattaaagt tgactggtca ttgaacaaaa aaggatttct aaagcccatt gactactttc
    30781 catgccaacg ttacataaaa tcaacgaatt tttgtcaagt aacatagaaa cacagtcaat
    30841 cattatgcca agcctcattg aatgggataa aacttgtcag ttccaagaac gtaccttttt
    30901 tcagttttca aacccttttc atcaagcttc aaagcccgaa ggagtatttt gtttccggat
    30961 tgagaaagct caaattcttg aacataagag gtgttttcag gacatagttc agatttttcg
    31021 ctatatgtca tgaaaagcac accatcaaat actcttataa gaccaaagcg attatccaat
    31081 ttaaaaacat gataaccttg catcatttca cacataattg aataagggta acttgtaacc
    31141 tcaaaaagca tactgctatt gatagaattc gataaactcc atgtcttgac acttccaatt
    31201 tcttctgccg acaacaacaa ataatctttt gaagtacgtt ttagagctaa aattaaaccc
    31261 gttctcagcc cagagatatc agctacaata atcagcactg agtcttgctt cagtatatat
    31321 cttattttag ataaatctat tgtatcgctc atactaagat aattcttgta agacggtaaa
    31381 acacgttttc caccaattaa gttaggtagt ccatatccat atttaccaac aggtacaaaa
    31441 aaagcaggaa gaagcccgtc aaatctagta agtgtatcag caacgtcttg aatgctatct
    31501 tctatagaag tggcggccaa cagcgagtca ttaaacgcag tcggtacatt ttgtttggga
    31561 gtttttgagc agccaatcaa aattaatatg attaataata aggattcttt acacatatta
    31621 ttttttataa gttctcatgt atattgggtc aacaccagga ggagtagttt cagtctttcc
    31681 gttaacccaa ccaggcacac tgaacggagg ttccattcgc agtggactat agaagaacaa
    31741 tgagccgttg gttcgattgt aatttccaaa agcaatctta actgccgcag aaaccgaagc
    31801 aaggaattgt tgaaattctc ttggatcatc atttgttatt ttatatggat ttgtataatg
    31861 atcataacgg ccgttacctt ttattgtaga tgaaaactga acaccgtctt tcctatcttc
    31921 taagacacct ttgtaagtgt ttccagtatg cttgtatgtt tcactttctt ttctgttgtc
    31981 tattatttcg gcagcggttt ctttttcaat ttgagggcgc cctccaaatt cagcatatat
    32041 ccctcttgca aaagccaaga ggtcatcatt aataaaatac tccgtccaca tataatcgcc
    32101 ccatttttga ccagcaattt caaaaaaaac aggtacgcgt acaaaatatc taggtgggcc
    32161 tggcgctggt actacagtaa cagatccatc tatttcaaaa tagaatatgt caaaactccc
    32221 gtcagggtca atttttatag tgggcatatt tccgacaaat acgtatgggg tattatattc
    32281 atcggcagga tccaccgcat gccacctacc caattgcgga tcatacatac gccagccgta
    32341 ttcgtaccag ttgagtccga agtcttcggt tagttcttta ccattgtaga gatatttatt
    32401 atcggacttc gagccaagtc cttcgatacg catgccgaag gcgtagtagt ggctttccat
    32461 cagtatatcc ggttgctgat tgggcgtcgg cagcagatca gcaaatgaaa ttcgcgtatt
    32521 gccaagatga tctttgatat cgtattcata ctgtagatcg ccggttccgg ttcttttgat
    32581 gcggccggtt tccgtaaaaa agtagtccaa tgtataattc agattattcg atgtatatac
    32641 gaatccgctg acatagtcaa tcgtggaagt aactgttccg ttggtgtata ccgtttttct
    32701 gagtttggct ccggttactg catatttcca ctcaatcctg ttactgctgc ttccgaattc
    32761 cactttggtg ggcaggttca ggtagttgta ttcgatgctg gtgatgcctt tgtttttatc
    32821 gagggtcatg ttaccattga tatcgtagca tatgaatggt agtgcgtttt tttccgcact
    32881 gtaaatgttc acgttatgga aggtgtgttt tggaggctac aatcatcggc taataattat
    32941 ttcttctgat tttttagcct tagctaactc gattctttta cgcaaatctt cttcccgctc
    33001 ttgccaatat ttcattttct catccgcgct cattccctcc aatcgctgat gtataatttg
    33061 ttgggctttg tgcttcatct ctacacaatc aaaattcttc attttctatc acctccattg
    33121 gtgaaaatat aataattggg ccatacccat tgagaatatt tacggcattg tacatcggta
    33181 ttttttcaaa atgcacgata tgtttaaaat tccaactgat aatcatggaa cattctgaga
    33241 cactagccaa agccacatga agagcatcct cttctgattt tttcgaaagt atcccggcgt
    33301 tgaggtatgc cttctgtaac tgtgtcgctt ccggattgag cgtgagcata gaggcatagg
    33361 ccagcatttc ctcaaaaaac atttttacat tctcaggcgc cagcgaaatt tcatcggcta
    33421 ccgtcggcga gatcacaagg gaaaaacgct gttcttttat ttgttcaaag aaagttttgc
    33481 tggctcgttc aaactccgga tcgaacatac cgccaaacac cgatgtatcc acataaacac
    33541 gattattttt cataccacct cgcatgctat taaattaatg aatatctcgt atgattccaa
    33601 tccaatattt cggcaagctc aatacaggta tttcgacaaa cccaatacaa gttttgatac
    33661 tcaccgatcg ggcgtatccg tcacggcgtg gctgatcata tgagcgctct gttccgccgc
    33721 gctcccgcac atacgcggga tacgaggcat ctgaacggat aatctatagt gaagatatcc
    33781 cccgaccggt cacgctgtca ggcgtatatc agcgtccagt ggggcaaaga ggcaacgaag
    33841 tagtctgccc cgcgctacga cgcggggtgt agcaggactt aggcgaccgt cgggattcag
    33901 aaggaaaaag gcggcgggag atcagccaga ggctgaaagc gcgcggagat tcaaaaaaat
    33961 ccggcagtcg gcaatgctca aaattgtatg ctaaaataaa ttgtgatata ggtaattgca
    34021 ttgcctatca aaagcgctgc gctggtctta ccaatagact gtttccaaat taatcgaact
    34081 acggttattt caattaatgc actaccggca aacataacca caaacgacaa tatccaattc
    34141 gctttactgt tcaaatcaga tagaaagtga ggcccatacc ctgtcactgg tatcattgta
    34201 aatgccatta ctgccgttcc aataactccc gaacatatat taccaacaga tgacataata
    34261 acagattgtt tttggcttat ccctatcatt ttctttagga aaaaagcttc aataaaaatc
    34321 gtcatcacaa ctacatacca aaaagcgaat atccctttta atatgtatat tgctggccag
    34381 accacattta ggacgctcaa aatcatttag tcccccaatc tttccaagta tccgtattag
    34441 gatcatacca ttgattggtt ctaaatggtc cactttcaac aatcccctct ctatctctaa
    34501 ctacaaaatt gtgtccaaca ataggagaat aattgtcagg catatgaaga ttcagaactt
    34561 gtgcactcat ttttttcaca aaatcctcaa aaccttcttt gggccaaatg atacctaaaa
    34621 acttctcaga tccagcagtt ttcatcctat tttcactaag cgttgatcta caaaaaccat
    34681 cacaattgtt atcctgcgta tcgtatttct ttgtaacgtt ccttcttgca gatgctaaga
    34741 gaagttgatc ttgttcgtgg gtcgtctgaa catacgctcc tttttcgtag ccccagtaac
    34801 cttctttttc aaaggcagcc aagttactaa aatattgtcc atctgtgttg ctttctttac
    34861 catccacaaa tccattttta gaaaacaaat accaccccgt ctcgtcgttt ccaatcaata
    34921 atgcagtatg tccactgaga cctgttccaa atggcgcgtt ttgatcaagc aagtaaatta
    34981 catcacgccc atctggatca acagttataa ttggattgtt acttaacgct aagtatggtg
    35041 aatgaaattc atcacttggg tcaaccacat gccatctccc tatctgagga tcatagaacc
    35101 gcgcaccata atgataccaa ttcagcccta agtcatcttc aagctctttt ctattaaatg
    35161 ttagtttgtt atccaatgca ttagatgaaa ttccttcaat tctcatccca aatggatagt
    35221 aatgagattc ctcctgaatt tccggttgac cattattatc atcatcggta aaagttactc
    35281 gcgtattgcc gacttgatcc gataacatat actcgtaccg taatgatcca tttggcttac
    35341 gcttaagtcg tccatgagtg ttagagaagt attccaatac accattctta tacacgaacc
    35401 ccgagacgta atcttgagtc aatgtcaaag ctccacttgt atacacccgc ttctcaagct
    35461 tcacccccga aaccgtataa gtccactcga tgcgattctg atgatcggcc ccaaattcca
    35521 cctttgtcgg cagattcaga taattgtatt cgatgtttgt gatgcctttg tttttgtcct
    35581 ctttcatgtt accattgaca tcgtagctgt attcgtgtgt agaagcattg gatgcggaaa
    35641 atgtaagttc attaccgtta tcggagaagt cgtatttttg attattggcg atgtcggcga
    35701 cggcgtcatt gaccgagtag aggcgattgc cgatatagcc gtattggaga ttatccatgg
    35761 taccgtaggt attggaagac cagttggttt ttccggctct taggagcgac ttgatattgc
    35821 cgttgcgatc ataggtcatc gtctcattgt agatgcccgg ggtctgattc cacgtagcac
    35881 cgctgtacga ttcgtactgg gcttcgctca gttggttgag tttatcatag atatatccat
    35941 aggcatgccg ggacgtaagg gatgtggacc atatctgctg gctgatattg ccgttgtatt
    36001 gcgcattacc tgcgatagcc gcatccggtg attcgtactt cagttccata ccgaaaagat
    36061 atgtccctag attagccggg tcgtttattt tcgtcatcca gccgcggatg ttgtacttgt
    36121 aattaaccgc ctgcaagaaa gtacttccgc tattagtgga atgcaaatcc ttttcgatca
    36181 gcgtgccgag ttcgttgtac acattcttgg ccagcattac ttcgctgtca gtgttgtttt
    36241 gttgatacgt tttgatgagt ctgccgccgt cgtcgtagtc aaatcgtttt ttgaccgtga
    36301 cggtggtgat agaggtcgtg tgtacggttt tggtcttgag tactttaccg gggaagtcat
    36361 actccgtatt tgaaacatcc gtcccaccta aactgttatt actctgcgtt tggatgaccc
    36421 ggccgtattt atcatagaag ctcacgctcg aaagccaggt gacgccattg agcgctgccg
    36481 cataagacga cggctgtttc acggcatctt ttttggcctc cagcgtcatc tcgccggtga
    36541 gcatgacctc gccctccagg ccgatctctt tggcagagat attacccgaa ctctgcgcct
    36601 gatttttgag cactaccgta tccggtgcac tcgcaaatcc cgcataaccc tgtacgcctt
    36661 ctaatatctt cgtcttcgct ccggtgacct tgcccttcag gcggtcaaat ttgctgttat
    36721 tgggaaactc gatatcgctc gcaaaggtat agtcatcacc gcccgtatta tcaaaattat
    36781 aatcatcata atacgtcacc gtccatacat cgcagctcgc cagatcgggg aatgcctgat
    36841 tggccgtata gccatgattg gtgctatagt tcagtgatga gcgctcttca taaaatttct
    36901 tacccgtcgc gctgccattt cccgcgatca catacgtgtc caccagactt tgcatgtcaa
    36961 cccgcgtcgt acatgtggta ttggtgtaga gaccggtgag aatagtttag taaaatcaaa
    37021 aaacgataca ggggagaagc ctctcccctc gcctacggct ttcacgccaa ggcgtgacga
    37081 tcctcggcgc ccttcccttt gaggggactg cccacgcgtg gggcatcgga tatatgagtg
    37141 tcaagcgcgt taattcctat cggaacttag gcggcggctt gtcccctatg tgggattcag
    37201 aagcaatgag gcggtgagag acgagagata gcgaagagaa agatagctca gaacgtcaac
    37261 tgatgattcg aacaccattg tgaccaagga agaaaaaacg aattacgctg cacccaaaaa
    37321 aactttaccc atttctagta ataaatcata taccgtatga gcaattatag aaggtaccaa
    37381 cgaccctgtt cgaatgtagt taattgaata tacaatacca atgagaacac ttgttacaac
    37441 agaatgcagg cccatcggcc aatgcataaa accaaatagc agtgaagaaa taataacgaa
    37501 agtgatcttg tttgcaaaaa aaagattcaa aaaacctcta aaatagattt cttcaaaaaa
    37561 acccgatcta atcgcgatca gaaaaccaac aaggaaatca acaaagacac ttttttttgg
    37621 tatttcgaca ggattcatcg ccaataaatt tataggaatt aaaaacttaa ctatagaagt
    37681 tgaaattgct tccacaagta ggatcaatat aaaaaatgaa aaaacataat agtatttcgc
    37741 cgaagacttt tttatgtttc caattgtagt ggtaatacct atagatttcc aagttaatga
    37801 tgtgtttttg attgcataaa tcaaaacgat aaatggaatc actaataaag tgatttgaaa
    37861 agcaaatatg tgttccggat gttcatgata gaatttaggc ccgtaaaaat gatttatcaa
    37921 aacatcaatg tttcccaaaa agaacaaata caatatcttt ttgttaagtg acaaatgttt
    37981 taccatgtta gccccgggct tgtcatctgg ttacgaatcg tctctggtgt cacaggtttc
    38041 acatagttct ttggggtgaa tgattcccga ggaacgaagg ggtccttctt gacggttaat
    38101 ggaactttaa gaccggatcg ttcgaggtat ttctgaaaca aatcctccat tatcatcaaa
    38161 ccaaggctgg gtttatattg aagtttaaca gtataattga aaccactacc aaagtcttta
    38221 ataatacttt tatcgttctt cattatgttt acgccttgcc cgccaggact atgcactgtt
    38281 gctccattgc ccaaacgcat tgaaaaattg attgaaccgt tatcatttga ctgaacacgc
    38341 aaaccgattg atttcataaa atcctcatta tccgtttgac cagtttcaat caaatctttt
    38401 aaagttactt tacctgctaa atctccttct tttcctaagt gttcttctgc gatttgctga
    38461 ggaataatta tacttggatt ccagttttta acgcaacagg ttattgatta ataagttgat
    38521 taaagacttc ataaggtttt tggaagttca ggactttacg aggtcttccg tttaataggt
    38581 tttggacttt cttgatttga tatctggata cattgttaaa gtcggtgcct ttgggaaagt
    38641 attgtcttac aagaccattg gtattttcat tagtgccgcg ctcccaaggg cttcggggat
    38701 gggcaaaata gactttcatg ccggtgatat gggtaaacag cttatgctca gccatttcac
    38761 ggccttgatc ataggtcagc gataatttca tttgttgcgg taattttata acttctttag
    38821 caaaggcttt ggcgagatca taggcatttc ggtttttcag cggcactaag atcgttgttc
    38881 gggttgtacg ttccaccaac gtccctaaag cggagcgatt atatttaccc acgattaagt
    38941 cgccctccca atggcctgga ataatgcgat cttctagtga ttgagggcgt tcttcgatgc
    39001 tgagcatatc ctctaaatta cgcacgggtt ttgcaccccg ggtatggcga taccggcgtt
    39061 tgcggttttg acgtaagcaa gagatgagtt cttttttgag cgttcctcta ggcaagatat
    39121 agatgtaggt atagatggtt tccgatgata ggcgcatggt catttggcga aaggagaagt
    39181 cttgagccag cattcggcta atttcttctc gttccacttc gcttagtctt ttataatttt
    39241 tcgtaagaat ctaactaatt attaaaatga aattaaaagc aattcaccta ataaaaacct
    39301 taatcatctt tgaaaacaca caaccatcaa acacatttga atcatacaaa gaatggggga
    39361 aagaatttgt gttaacaaaa taaataagac gtaaccaata accaccattt gacaacgaat
    39421 acctatttaa cggtatttga atgctgactt gcaacgtatc tgacggcaac aaccttaccc
    39481 tgcttgataa aatatcatcg tatgtgttcg tactttcatg ctggcgtttc gaattgatgc
    39541 tgtttgaacc aatagcttta ttgtttaaca gtgtgtcaat atcaaaatga taaggcaaat
    39601 ctcttacaag acacatctct tgagcagaat cataaagtaa gacattaaaa ccattcaaaa
    39661 cgacatctcg acaatcgtat ttgctttcca ttaaaggccc gtttccaaaa acttcaggac
    39721 gtggtattgt cttgttaaaa gccgtgtaaa caattaatga gtcattagat ctatttatta
    39781 aatagacaat agttgacaag gtatctgaat ttgaattaac tattaactcg tcttgaatga
    39841 agtttatggt taattttttt tcctcacatg aagaaaaaac aatcaacaaa cacattaata
    39901 aaaacggagt aaacctaatc gtattcttca agagcttttt ggatatcatt gatatatcca
    39961 tcccatttat gtgacgttgc ataattcaac tcccaagcgt atgctgcttt ttcactcatg
    40021 ttatgtgcat catctgaatt ttgagaatta ccccacctaa catattcacc agaaaaataa
    40081 tgtattgagt gtgtaagctc atgccctaat gtcaaaaaga gtttctcagc agatgcaaat
    40141 gctgatcggc tgattatatt gtatgttcta aaacctattg acccatcaga agttttgaaa
    40201 cccgaacgct gaattactcc tgtatatcct gcactcattt gaccatcttt caaatcatac
    40261 atactaccat caataacacc aatccttcta ctaccaacag cttttaagtt ttcaggtgtc
    40321 gttattctaa catcagagtt ccctacagct ttatgaagtt tttgtaattc ttcactattt
    40381 ttaattgctt tcgaggtttt cttggctaat ttcaatagat tttttgggat cagaataacc
    40441 tgctttaata gctctttgac gcgcaacatg aagttgagat ccgtatgata gagatccacc
    40501 gagagcacca attcaagcat taagtgctgt attcagtcca aaatttgatt ttccgtcgga
    40561 aatacgcgaa ctatataagc tacctgtcaa acttgatgct attcctgatc gaattgaacc
    40621 tcctaacgtt actccagctc cacctgcaaa tcctgttata accccccccg gctctggaaa
    40681 actttaaatc atggtaaaga aaataactct ctacgcttag cctgcttaaa cactatttcc
    40741 gggatgacaa cttcactacc attaattgat agacccttaa tttcaaaatg taacttcttt
    40801 ttggggtcag gcacgatagt tttgaatttt atccaaaaac aactcgtgat tgaatcctct
    40861 aacatgatag gctcgacagg tagtttccgt tcaatcggtc tagtatcgct cgacgaacat
    40921 tcgtaaggtc ctataaagcc atatggcaaa tatttagtac tcgaatcagt ccaaaatata
    40981 actttttgtg gatcaaagcg taaatcggta caaaacggct tcaaagatat cccaactgaa
    41041 aactctgagt ctatcggagt tgattcagaa aacgggaatg gaattggaaa tataagaaaa
    41101 cccgaatctg ctgcgttgag aggaaaaacg cgataagcaa aatttttctc aacgatcatt
    41161 tcaccaacat acggctcctc tactatttta ttaaaattat tgttttccag atcaagttta
    41221 ctgaagcgac tattggagat tcccacacat cctgttatga aataaaaact tgcagcaata
    41281 ctaaatagtt gcatcaattt gaatgtcttt tgtttcattg aatttacctt tcaattgaat
    41341 tccaattttg tgggtagaga atgaaaggaa cacaaccaag tggtttataa atagatatat
    41401 ccgaaaatta ttatttaaga actgagcatc ctaatgtgca acctaaattc gatcatcttt
    41461 aaagatggct cagaaaaaaa attcacacat atacaaccgc ctatctacac tctatgcatt
    41521 acaaacaaaa cttgtacaga agtgattatt gactatttag atgataactc aagaagaaaa
    41581 tcatgcttct ctttattcgt tttgctcaaa aaatcgtcag cgattagtct atagaaagcc
    41641 tctttgtgaa ttttattatt tttaaaatta ttcgtatcat aacaagtgat acgtgggcca
    41701 tgcacatata cgtttttttc tgcgtttact gaatacttat attgactact atccgttatt
    41761 ttatttacat gaattatcct atctttcgaa taagaaattt cgaatgaagt ttttatagct
    41821 atcgaatcat atgtttcaat aaaaccaatt attgttcgcc cataaatttt tcctggaaac
    41881 tcaaaaatgt aatggtttct gcccatggtt gaataactat tcttctcttc atcacaactc
    41941 agtattctca atagaacaat aacaattaat attttcataa gctcaataat gattggttaa
    42001 attatacgaa aataaatccg gtcttacacc catagtatag gctttcgttg ttgcatacaa
    42061 tatataagga tgtatcccaa tattaagtat tccattaacc caaagagaca atgaagccat
    42121 atttgcacaa ttaactgtgc ctccttggta aggcaaaaat cttactaaac cccgaatagt
    42181 tgcattcatt ggcatttgaa accattgaaa tctgctatag ctggtaaaat attcattcta
    42241 tgctttctta taaaaaactc aataaaccta aagtgctcgc agtcatctta gtcgcaagca
    42301 attgcactaa ggacccgtct tatttgaaac acaaaacatc tatttatcat cgtcatcaaa
    42361 aagatcagga tgtaaaggaa tagactttat tagtctttcc ataagatgat ttttatgaca
    42421 aaaaagattt tgcttttctc cacttttaat gtttggttga attgttaaga ttgtagcttc
    42481 ttctaaagga agtgttttcc cacaaaaaca acaaattact ttatcactca agtttccatc
    42541 catagttttt agctattctg ttaatagatt ttagagaaaa attttccatt attgtaatta
    42601 gtccgttatt gccttggaca acaacattaa atctgccagg tgatacttca tgtagaaaga
    42661 ctgttgcacc atctgcttga ctaaatgtcc tacctaaagt tttgtagtat ttataccttc
    42721 gaccgataat actccgcctc ttgtagcatt agaccctgca atcctttttg agcatgatcc
    42781 gttgtttgca tccccgcaac catatggtat tccggatttc ctattgacgt tatttcaatc
    42841 ggctttccag tccaagggtt tatcttgctc tcttttgcgt aattatacct acagtacctc
    42901 caatgattgc gccactgatt ccagtataaa cataatttgc atccaaagct gcatctaaag
    42961 ccgaaccaaa atcaccggtt ctgaaataac ctcctgcgaa attacctacg aatcctcctg
    43021 ccaatgatcc tacggcgcca gcaaaagcag ttttaaaaac agggcttgtt atagaagtac
    43081 cacctacatt tacagctaat gaagaagcaa agtttcctgc tccgtagccg gcagcacttg
    43141 caaaaccacc tacgatagcg ctttgagtga tttggccagg attgtctccc cgctatgctg
    43201 aatttgcacc tgaccccaac atacctccga taatggcacc agttggccat ccaaagatac
    43261 tcgccgtagc ctggccgcct cctacgagaa aaaagctaaa tcctttacca aaattatcaa
    43321 tcttatctcg attttggtag gcgttataaa tcccactccc aacaattatc agtcccgcaa
    43381 taagaggggc aatctttcca tcaggatcat ttcgtaatgc tgggttgttt ccgacgtata
    43441 aaaatggact actaaactgt cctgccggat ccatcccata aaaccgcatc agatcgctat
    43501 cacacatccg ggctttgtag ttatgcaacc cgctcgtctt atcatattcc tgaccggtgt
    43561 acatgtagtt tgtattgggc gttaccggcg cgagaatact tccaaacgca ctgtagtcat
    43621 atcccgtaac tgtcgtaccg tccactaccg cacgtgtaga accgagatga tctttgatca
    43681 cgtatttcca tgtggatgcg ctaccatagg cgatgatgcc cgtaggtccg taaacgtatt
    43741 cggtgactgt tcctgttttc atacgctcat ggatcgcatt cataccatca tgcaaataca
    43801 aagcttcact ggtcacaccg gataccacct ctttgcggta tatgcgacgc tccgtaccat
    43861 cgtattgaaa gttcatgcta ctcgcaccga tagctatcgt gcgggtacga tggataaacg
    43921 gatcgtaggt cagcgtaccg atccctttgg gtgatgaaga agtgatattg ccggacgcac
    43981 ataagtacgg ataccctcta tcggggtgat gaataagcgc ttgatccgtg taccgacgcc
    44041 cttttaacgc catgcgcaat gccgagagcg ggccttcttt ggttaaatca gagcggaacg
    44101 catagcccat aatcctctta gaataagcgt ccgttatcag actcaagtat ccaaatcctt
    44161 cgtcactagc cacataagta atatcgctca cccatagctg ttcactacgg gttggtggcg
    44221 gtagagcgct aattaaatta ggatattttc gcacccaatg acgtgaatcc gtagtttgta
    44281 cataccgccg cttgggtttg atcagcatac ccgattcccg taacacggaa aataatttat
    44341 cgcgtcccac gcgtaacccc ttttgctgaa aggatgactt gagtaccata atacagcttg
    44401 cgggtaccta tacgcggcat ttggctccgt atctcttggc tcaggcggcg aaccgtttcc
    44461 tgtacctctt ttgacctagt tccgcgcttg gcttgcttat agaaagccga acggctgtaa
    44521 cccaaaacac cggctattcg cccaacgccg tatcaccggc tcccttgatc gccatagctt
    44581 cggacagttg ggctaagtac ttttttcgta tgtcgattcc cagctgcgcc tcagctatat
    44641 ctatggcgcg ttgcaaaatc tccttatcct cacgggcgcg ttccaaggct ttcaccaatt
    44701 cccgaatccg tttaccgggg gttgctttct tctgcatagt tgaactccaa tctaatcgcc
    44761 cgtgtttaca aagccatacg tggagcgttg attggacctg aatcccgtac ttcgacaacg
    44821 cctcttttcg tgtaaaatcg cctttttcta tcgcatcaac aacctgtaat ttgaacgcta
    44881 aactgtaatc ccgttgcgtc cgcttcacat acattctaca tccttgtcct tcttgcatct
    44941 gctactcctc ctgtggacgt atttcaggac gagacatttt gataaataaa aaaacccccg
    45001 cacttcacag tacgggggtt tttgtttaca aatcacaaag tactactttt tgcagcactc
    45061 tttgggagtg catactttgg ttccgcagtt ttctttatta gggcacgaac cgcaattatc
    45121 ctgacccata cctgcaaaaa caaaagacgt gaccatcaaa gcggccgcag cgacaagcat
    45181 taattttttc atatataaat ttcctatttt ttaaaaattg atatcatagc aaataacata
    45241 ttcgccatat acaaaatact tttgtgatgt ttatttttat gcgttcggga tgacagcgtt
    45301 cggaggatgc caaatgatgc ggaggatttc cggattatat ccggattccg gtgcggaaaa
    45361 aatctgatgt tgtatctgtt ttcctggtga aagggaaatt gattctactg attcataaat
    45421 gcagcatacc tgacaactta caaccggagg gcacgcttct ttagggcaat tttttttgga
    45481 atcatgatgt tgcgatttgt tgcacgcacc cgtacttgat ttaccgcagc aatttttctt
    45541 ggtttgaaaa atcaacgacg atgtcatcgg cacattagtc gccacaacaa acgatagtgt
    45601 cagcaataaa acacttaaag aataaaaatc gtgtgtactc ttaaatttca tacgtaacta
    45661 aagattactc ttcaaatcgc ttaaaaacca tgatccggat caggaaacgg agataaaact
    45721 attgtaacta cgaaaaaaca agggtttggt ttcaaaaaat cgttttttag tgcgttaaat
    45781 accttgtttt ggtaatcttt ttttcatgac tttacgccag atcggaggga taacgactaa
    45841 ccaaaagcag ccgtaataac cggtgggaag attaggactt tcccgatagg ttcttagttt
    45901 ccaatattca cgggatgcga tatagtgatg atcggcgtga cgtgtcaatt cgaccaaagt
    45961 ccaacggctc caccgatgat tactttgcca actatgctcg ggttgaaccc gcgaacccac
    46021 aggcctttgc aatccccaat gccggatgta gttcacatac tccagcaaaa aaacggcgaa
    46081 ggcgctttga atcaaccagg caaacaaaat aaatttcccc aataggaaat aaacgacgat
    46141 ccatgaaact atttgtatta ctaaaaaaac caaaaccgga ttgagccgcc atgaatttgc
    46201 acgaggactc atgattttga aagcgtttat aaattggccc ggaatcgtcc tgattaaaaa
    46261 agaccaaaaa ttttcattct ctcgggctga agccggatct tctaccgtag ccacgctttt
    46321 gtgatgtccg cggacgtgtt caaaataaaa atgcatatag ttaaccgtcc acaaaagaaa
    46381 aacacccgta acgcgttgaa acgcgtatcg gcgatgaatc aattcgtgcg cgatcacgat
    46441 ggatgaaatg ccggagttca gaccggtaga aatcgaagca agcacggcga aataattcca
    46501 cccgtctcgt tggatcagaa gcgccaaact aagcaaacaa acgatctgca aaaccgcatg
    46561 cgtcatcaaa atggcatcaa aataaaacgg atcggaatct tctaaattat tgctttccga
    46621 ctcccccagc agccagtcta acaaaggata gatcaacagt ccgactacag cgcctgacct
    46681 cgcaaagtga ccgccaagcg tattacccgc aacaacactc aatggataca gaaaactaaa
    46741 aacgtatacc caccggcttt gagttttcat aataataatc ttaacgcgtt ttttttagtg
    46801 ctaatcagta cagaaacatg ggtttgtgca tgacattaaa aactttcgga cgatagtttt
    46861 ctacgtcata aagttctttt acatccacat tgcgtttgcc ttgtatgatc gtttccgcgg
    46921 gtaccgtagt atagcgccca tcctgcaaag ccgtcatcat gccaaaacgg tttttggtca
    46981 aaaggtcaat cgccaatccg gcaaaattca tggccaccat acgatcaagc gcatccggtt
    47041 caccgctgcg catgagatac gcgagagatt ggtagatcat gtgctggcct gtgatttttt
    47101 tgatagcctc cgccgtgatt tcgccgatgc ctccgagttt tttatgcccg tatgcgtctt
    47161 cctgaccgta ttcgatgatc ttgccgcctt ccatggtagc gccttcggat atggtcatga
    47221 tcgcataatt actgggattg ttccttttat cgttcaaaag catttccgat agtttttcgg
    47281 gatcaaacgg cacttcggaa ataatggccc gatccgcatt ggccagcaga gctgaaatca
    47341 acgacgtttc cccggagttg cggccaaata actcaactac cgctatacgc tcgtgtgagc
    47401 cggccggtgt tctgagcata ttgataaagt gtaccgaacg cgtgacagcg gtagaaaatc
    47461 cgatacagta atccgtacca tacacatcat tatccatcgt tttgggaatc gctacgaccg
    47521 gaaaatcttc gttatgcagg cgaacactat aactcagcgt atcgtcgccg cctatcgtta
    47581 ccaacgcgtc aatttttaaa tgcttgagca cgcgaaaaat atgcggagta aaatcaattt
    47641 ttgttttgga atccagtgta tcatgttcgg gctttttcaa aaaatcgggc acatcgcttt
    47701 tgttgacttt gccgggattg gttcgcgacg tatgtaaaaa agtaccccca ctgcgatcaa
    47761 tcgtgcgtgt attgataagg tccagcggtg taatccattt tttactgcct tccggatcat
    47821 caggattgta tctcagcaat ccaccccaac cgcttcgtat tccgaccact tccataccgg
    47881 catgaatcgc atcataaacc aacgctttga tgcaaggatt gagacccggc acatcgccgc
    47941 cacctgtcaa tactccgatc cgcttcataa atacacctca ttaggttata aaatccatat
    48001 aagatgagga ttcccgctac gaaatgacat gagaaatgtt gggaaatata acttatgtgc
    48061 tgtttaaata gaaatggaat taagtaaaag tattctagga tagacgaaaa atcaggtgtt
    48121 ttttttaccg tcaaaaaatt ccaattctgt aggtttggtg tcgtcccatt cggatgcacg
    48181 catgacgatt tgatcttttt ggcgaacgat cgcgtatccg cgttgaagtg tatttttggg
    48241 gttgagcgtt tgcagttggc gagctaacgc ggtgatcgtc tgtccggaaa cgataagttt
    48301 gtgacgcaaa acgcgcatca tagttttttc aaaatcatcc acttgctggc ggtattgacc
    48361 gactatgttt tccggttgac gaaaagcata tctgttcttc agattatgca actgtttttg
    48421 ataatcgctg attcgttttt ttacattttg cgacagcgtg tattggagat tgacgatata
    48481 acttcgcata tcttcctgat ctacggttac catctccgcc gccgccgaag gcgtggccgc
    48541 acgataatcg gcgacataat cactgatgga gatgtctatt tcatggccga cagcgctgat
    48601 cgtcggtatc gaacaggcat acatagcccg cgccgtcacc tcttcattaa aagcccataa
    48661 atcttccaat gacccgccgc ccctgcctat gatcaaaaca tccacggctc cgtatttttc
    48721 aaattcgtga attgcatgcg ctatttcctc ggcggcccct tcgccttgta ctttgaccgg
    48781 atacaaaatc aattcgatcc atggcattcg tcgccgcgca acgctaagta tatcgcggat
    48841 ggccgcaccg gtaggagaag ttatgatgcc gacgcgctga atatattttg ggatagtttt
    48901 tttatgcgag gtatcaaata atccttcagc atagagcttt tgtttaagtt tttcgaaagc
    48961 ctcctgcagc gctccaatgc cctcttgttg gattttttga acggttattt gataataacc
    49021 gcctttttca tataccgcta tgtttcctcg agcaataact ttttttccgt ccttcaaagc
    49081 atccgattgg gacgcggaag aacttttcca tatcacagct ttgatctgtg cgccggcatc
    49141 tttgagtgta aaatatacgt gaccactgga ggcgcgggtc agattggaaa tttcgccgga
    49201 aacggtaata gcagggaatg aatcttcgag atgggttttt attaagcggg ttatttcggt
    49261 aatggagtaa ataacaggct ttttctcctg ccgttggccg agatcggatg aattctcatt
    49321 tgaaactgaa aataaatcca ttctgattac agcttcatta caaaattaag cccgccttgg
    49381 aaaaaggcac cgtttatttt ggagtggacg gtgcttttga atggagtagc cataataccg
    49441 ccgccgatga acggggtgag atacatacct tcgtctgttt ctagtctata acttacccgt
    49501 ccaaatacgc taaaaaactt gacatccggt cccggacgat aatacggttc gtccgtaata
    49561 ttataaattt tcgcgtcgtg ctttttaaaa aaatagtgcc cgccgacccc taatgtccaa
    49621 gcaaaccctt gtttatattt tgacaaatag ttattcatac aaatatgagc gccaaaacca
    49681 tgtaccattt ccgttttatt agtcaacgag gtatctaaaa cgatatatgg tgattcaaat
    49741 tttactcgtc taaatgaata acccgcttct aaactgtaag tgcttttatt ggatttgccc
    49801 acagcctcca cgactacacc tgtaatcccg gaataggatg tggtagcgcc aataagataa
    49861 cttgttttgc ctttttcttt ttttgtttcc ttttttggct ctactttttt ggtcggtttg
    49921 cgtcgcacct gcgcatcggc tgataaccca cacacagtaa aaagaacgat gaaaataaaa
    49981 catccgatac gttttgctga actcattttt tttccagatt tttaacttga aatcctttag
    50041 gcgtttcttg tttaaagaca tccgagttga tggaatggtt taatcggaaa tccgagaatt
    50101 cccaaatcgt tttatcaccg tcggcttcac taagttccgc ctgatagggt aaccacagat
    50161 catcccttac gtaaataatg acgctttcca ttttgtcata aaacttgctt ccgcgcttgg
    50221 gaacaagtgt cagtttatat gaaccaaatt ttttttgaac ttgtttggtg tccacttgta
    50281 catcaaaata tttttcgagc atctcaagtg aattaccaaa acctaataaa tactgactga
    50341 aattaatttc attatcagac gtcgtcgtaa cggttttatt ttttcgattg ataatccata
    50401 tcgaattgtt gtttacgata tattggtttt cggcttctga tgaaggattg agcgcatact
    50461 tgcccggttt ttgataataa aaatccgctt ttgtaacaat ctctttatcc ataaatgaag
    50521 attgtttacg ttgtttcatt ttagcctgaa gagaaattgt cgcttcattg gctgtccgca
    50581 tgctggaaat aactaccttt ttttcttcat cgccggatcc ggcatgtact gtgaatgaca
    50641 aagaaaaaag aacggtaata caaagactga ttattcgttt ttctgatttt ctcataaata
    50701 tctttcttga tctgattttc cgttgatgaa ttccggtgaa cctacggaat ccatatccgc
    50761 tttttgataa acgtaaacgc atacaaacat agtatgataa aaattttgat tcatgcaatc
    50821 aatcataatc aacacatcta aaacacatcc gtctaacaac aatgattaca tcgggatcag
    50881 gacgacataa atcaacagga aatgtttttg caaattacgg tatctactcc tacatttcga
    50941 ggattgtatt tagaataata agaaaaaatt catggcatta cgaaaactgg cgtatgatga
    51001 aataaagcag ctccgctcaa cacccgaaga attagtttac aaaacgcgat ttcctatata
    51061 cggtgttttg gaaaacatac gtagtatgta caatgtcgga gcggcatttc gtacatcgga
    51121 cgccgcgtgt attcaagagc ttattctctg cggttatacg gcacaacctc cgcgctcgca
    51181 aatcgaaaaa acagcgctcg gcgctacaga aactgtccca tggcgacact ttaataacac
    51241 tcttgatagc attaattatc taaaagatcg aggcgttaca gtcgttgctt tggagcattg
    51301 cgaagaaagt aaaaatttat taactacgga gttccctttc ccggtcgcgt tatttgtggg
    51361 caacgaagtg gatggtttaa acgaagaaac cgtcgctcat tgcgatatgg cttgtgaaat
    51421 accgatgttt ggctctaaac aatccctcaa tgcggccgtt gcttatggta tcgctgtgtt
    51481 tgaattgacc aggaaatact tagttttgca agagaaaggg taatgctatg cgatcattga
    51541 tggttctttt tttatttatt aatttgacaa tttacggtca ggagttttcg atcggttgga
    51601 ctaaagctag tacagagagc ggatctgagt ttaaaaaata tgaaccccaa accgcgtgtt
    51661 tttttataaa acaccggcgc ctttacaacg aggattttat gtggggttta ggattagata
    51721 tgatgtcttt tcgtaagtcc gcatctccag aacgcacgat aaagataaaa ggaattaccg
    51781 gtgaaatagg ttattatttc cgacaaatac ccggtttgac cgtcttaggt tcaacaagct
    51841 ttgggctaat gcgatgcgag tacgacttca ttgatcctcc agcagatttg aaaggctctt
    51901 caggaaccgg tgtttctata gaaccctccg tatcaatttt tttcccctta agtaaaactc
    51961 ttattttttc accgtccatc ggttatcata tgctctttga taagatgaat tttaaaacga
    52021 caaaccctat aaaacaacct attggaaaac tcgattctca catgttcgct atgacctatc
    52081 gccttgcctt gtcttggcgc tttcggaaag aataacacaa tgatttttaa aaaaaacaca
    52141 tactctaatg atgaaataaa catacaacga atcagcccgg atattgattt gtttaaggaa
    52201 ataaatcgac tgcgcaaaga tttgaatgcc gttatccttg ctcactatta tcaggaatcg
    52261 gaaattcagg atttggcaga ctttgtaggt gatagtttag aattagcaag gcaagccgcc
    52321 aaaacgaaag cggatgtgat cgtttttgcc ggtgtgcatt ttatggccga gaccgctaaa
    52381 atactcaacc cggaaaaatt agttttacta cccgatctta aggccggatg ttctttagcc
    52441 gacagttgcc ccactgatga attcaaaaaa tttcgtgaag cgcatcccga tcattttgcc
    52501 attacttaca tcaattgtac ggccgaagtc aaagcattaa gcgatctgat ttgtacatca
    52561 agcaatgccg aacggcttat cgctcagatc cccgaatcta cacccatctt gttttcaccg
    52621 gataaaaacc tcggcgctta tatacgtaag aaaaccggtc gccccatgat tttgtggcaa
    52681 ggctcatgta tggtacatga gattttttct gaaaaaaaat tagtacaact gcgggcaaca
    52741 caccccgaag ctaaaatcat cgcacatcct gaatgtgaag agaccgtact caatatggcg
    52801 gactttatcg gatccacaag cgggcttctc aaatttgtcc aacaatcgcc ggatcataca
    52861 tttattgtcg cgacggaggc cggcattatc catcaaatgg aaaaagcatg cccggacaaa
    52921 acatttatcg cagcgcctcc caataataac tgcgcttgca atgaatgccc gcatatgaaa
    52981 ctgaatactt tagaaaaact atatctctgc atgaaaaaca aaactccgga aattacactt
    53041 ccggaggatg tacgcacacg cgcattaaag ccgattttgc gaatgcttga aatgagttaa
    53101 cttgttccaa taactcattg tcataaacgt ttatttttta ctcagtctct tttttaaaac
    53161 attaatattc ttttgtataa catccgcatc gcgttgcgca ggatcaagcg caagtgtttg
    53221 ctctagatga tgtatcgctt ttttgatctg ctttttatcg ttcatatata cgatccctaa
    53281 actgcgatgg atatttgcgt tgtttggatt caaagccagt gatttttcaa acaacgattg
    53341 cgcttcttta tgttgttttt ttcgtttaag cagaataccc aaattgagat atgcatcgta
    53401 taaattagaa tccaaaacaa tcgctttacg ataatgtatt tcggctttga cgtaatccaa
    53461 gttgtcatca tatccgacag cacatccgta gtgtactaag gcacggagcg aatctatttt
    53521 taacgctgat tcataagcca ccaacgcttc gggtgcgttt cctttttcgc ccaacactac
    53581 acctaagtta acccacgcat ctacatattt tgaattgagt tccaaggctc tgcgatattg
    53641 taatacggca acatcccact cttttcgcgc ctgataaaca tttcccaaat taaaaacagc
    53701 gtcggcattt tcgggttgaa attcgatagc acgattatag ccggtgatag ccgaaggcca
    53761 atggctgtta cgataggcca tattagccgc tcgaagatat accgccgata tatttttttc
    53821 cgattttagc aaattattat aatccaaacg acgggctttt tcgaaaaaca aaatcgcatc
    53881 ggaatcgtta tttttttcaa aagccgaaac gcctaaactg taataaccaa gagcctccca
    53941 tttttgaaca taaagcgcat actcttcgtg atcactcgcg atcgtccgca atctttcatg
    54001 ccccagcaac acaccgtttg gactcaaaat atttttttct gtaaggttga ttcgttggat
    54061 taagttttca accgcacggt acatggtttt atagaaatcc gtgtagtttt taattggtcc
    54121 atagattcgc tcttttgcga tgtttacatt accgttatcg cccaaaagtt caaactccat
    54181 ctcgatggag tcgcttcgca tacaataatt tcctcgcaca atgaagtctg ttttatcatc
    54241 aactatgaga tctaatttct ctgaatgttt atcacccgat ataacgcttt tgttttcaaa
    54301 cctattaaat attgtatctt gtgaaattat tgtcaattct ctagtggcgc tcaacacatc
    54361 cgaaagcata tagcttagat aataagttgt tttattatcc gagctacaag tcgatgtaaa
    54421 cggaagtact gcaatttttt tattttgtgc tgtgagagta tatgcgcata taaaaagtat
    54481 cgtagcaaaa acaaaccgtt tcatgcctta agacctccat ttgcgaccgt ctttgttcaa
    54541 taacttatcc gccgcttccg gcccccaaga acctgcaaca taattcggaa actccgcttt
    54601 attgtttttc caatgttcca aaaccggcat tacgagccac caagctacct cgaccatatc
    54661 tttacgcata aaaagcgttg cgtcgccgcg catacagtca agtatcaatc tttgataggc
    54721 ttcggcaatt tccacctgaa atgcttcttt ataattaaaa tgcatgtcta ccgactggag
    54781 ctgcatttcc ggacccggta ctttggcttc aaagctcatc gtaatacttt catccggctg
    54841 gatttgtatc accaatacat tgggcgccac atcatcggcc gccgtctggg taaacatcat
    54901 atgcggtgtc cgtttaaaaa agatcgctat ctccgaatca cgacgaggca ttctttttcc
    54961 agtccgcaaa taaaatggta cgcccgccca gcgccaatta tccacataaa attttgctgc
    55021 gacataggtt tcggtatttg attgcggatc cactttcggc tcggtacgat agccgggaac
    55081 cggtttgcca ttgatctgcc cggctccgta ttgaccgcga acaaaataat cattcacctg
    55141 atctggtttt acgggacgaa tggcgcgcat gactttgacg cgctcgtcac gtacagcatt
    55201 ggcatcaaat gtagtaggcg gttccatcgc cacgagagcc atgacctgaa gcacatgatt
    55261 ttgtatcata tctctcaaag cgccggcatt gtcataataa ccgccccgat cttcgacccc
    55321 caaagcttcc gccgctgtta tttgaatatg gtctatataa ttgcgattcc acaaaggttc
    55381 aaaaatcccg ttcgcaaaac gaaacgccaa tacgttttga acggtttctt ttccgagata
    55441 gtgatctata cgatagacct gccattcttc gaaagcttta gccacttccg cattgagtgc
    55501 acgcgctgac tcaaggtcac gaccaaatgg tttttcaata ataatgcgcc gccagtaatt
    55561 acctccacgt acttgcgaca tattgtgctc agccaatttc tgacatatcg tagcgaatac
    55621 tgtaggcggc gtcgcgagat aatagatacg gttatcatta caggcacgcg tcgtacagtt
    55681 gtttttgatc agcgaatcca tagtttgata taacgcattc tcatcaaaac ctccgccgac
    55741 ataaaaggag ttttgaacga attcattcac gattttcgga tctacggcgt cggatttggc
    55801 aaattcacgc aacgccgaac ttaccatttc acggtatgct tcgctactca tctccggttt
    55861 gccgcatcct atcactgtga atttctcagg cagcaggcct tctttggcgc tattaaaaag
    55921 cgccggtaaa attttgcgcc gcgccagatc accggaaccg ccgaagataa tcaccgaaca
    55981 tgtaccggca cgcgcatcgg atttgacctg tcgcaaagct tctgtgacgt gagctgccat
    56041 gaagagcctc cagataaaag ttatacagtt atttcaaatc aatatgataa caaacaatca
    56101 tccgttttta agcacaacga agtgtgatca cggtgatttc gggtggagaa ccgatacgca
    56161 ttggcggacc ccaataaccg gttccgcgat tgacatacac ccacatatta tcatgccggt
    56221 gcagtccttt caaatacggt tgcgccatcg ccgccaatag attacccgga aaaaattgtc
    56281 cgccgtgtgt gtgtcctgac aactgcaaat caaaacccgc tgccgatgct gccggcgcac
    56341 ttttaggttg atgcgcaagc aatattcgcg aataatcgga tgatgtgcca tcaatagctt
    56401 ttacaggatc ggataaatgc ccgggaatga aatccgttgc cccataatcc gttataccgg
    56461 cgagacaaat ctgctgattt tcacgatgaa taagacggtg ttcattgagc aatacatcaa
    56521 atcctaatct tttagcttca tcacaccaag cttttgcccc ggagtaatac tcatgattac
    56581 ctgtgacaaa aaaaacacca tgcggtgctt gaagttgctt cagcggttcc acttcctcac
    56641 gaagccaagc taccgtaccg tcaaccaaat cgccggtgaa cgccactaag tcaggtttta
    56701 gcgccattac atgatcagta acacgctgga caaattcgcg tttaatagtc ggtcccacat
    56761 gaagatctga aaactgtacg atccgaaatc catcgaaatt tttaggtaga ttttgaagcg
    56821 gtacttcaat cgcttcgact accgggtcat tgtgcgcctt aaaaatacca taccctgcaa
    56881 ccgccgtaca aaagccggcc acaccgagat ttacactttg aatgagaaac tgtcttcgag
    56941 tttcgtctgt aacggtttca ttgggttttg tgaacagcca tgtaaaacca tggtgcaacc
    57001 atccacctgc atcccgaagt aaaaatgcaa ctaataggag actgaacaat ccaagactcg
    57061 aatatccgca ccacagtaga acatccaaac ttatgctttc gtgctgacgc tgcaatacca
    57121 tcataaatcc gccaagcggt aaaagacata aaacagcgat cactatccat ccgatataga
    57181 ccggtaaacc aaaatgagcc atagccggta cgatgcggcg acctacatac caataacctg
    57241 acataatgat ggccgtccac gtcagaaaaa accagataaa tcgcattaaa ttaattagta
    57301 tccgataatt ttgattctaa aaaataaggt cggatggatc atccgacctt ttataacttt
    57361 cagaaattct attcgtttac tgtttgatcc aggttttttc aaaacgacgc gtagcactgg
    57421 catgaagctc aaatcccatg acattatccc gcattggaac gatcgttaac gcatgagcta
    57481 aagtaaccca tggaaaatct ttattaaaaa cttcgcacgc ttttttgtat atatccgctc
    57541 gtgcattggg atcaaatgtt tgacgaccgc gttttataag attgtgcatt tcttcagaag
    57601 cataaaacga tacattggta gacgattgtt gtttggtaat atctttatcc aaaagcggat
    57661 aaaagaaaaa gtgcggatcc ggagcatcac cgacccatcc ggaaacagac atctcatgtt
    57721 ccccgcgccc gcgccgacgt aaaaattcgc cccagtttga tattttttct tcttccgcgg
    57781 ttatacctac agctttaaga tcgttgatga tcagttccgc agctaaaggc gggttgggca
    57841 tataatcgcg aaccagcggc aaaccccata gtttgcattt gaatccgttt ggaaaaccgg
    57901 cttctttgag cagttgtttg gcgcgttccg gatcataagg tgctgcctgt atctgatcat
    57961 tgtgacctaa aagagtagga ggaaccgggt ttttggcctg gctaccgagt tgtgcaaata
    58021 cttcgcgtac aatccgttct ttgttgatcg ccaatacgat tgcctctcgc actttgggat
    58081 tatcaaatgg cttttttctc atattcatac ctacataagc cacattcata ccgggctgtt
    58141 tggttacttt tactccggga gttttttcga tatccgcgag atccttttga gtcggcaaac
    58201 ccatcatatt gatcaccccc gatttcaatg caagccaacg ttcatgcgga tcaggtacag
    58261 gtctgaagat caccgtatca agatagggac gcccgtgcca atacccatca aaagcggctg
    58321 tacgcactga gccgtccgta tcccagctga caaatttgaa aggaccggcg ccgacgggat
    58381 ttttgctaaa ttctgcgccg tatttttgta cggccgcagg acttactatg gctaaaaaat
    58441 tgagcgacaa gatattgaga aacgtggcat cggctttttg taaaacgatt tttaccgtag
    58501 aatcgtttat ggcttcgatg gattgaaaaa tattgtccat attaaaattt ttccaaagcg
    58561 catagccact gctgctttcc ccttcgccgc ccgatttttg gcgttcaaat gtaaaaatta
    58621 cagattgcgc gttgaacggc gtaccgtcat gaaaagtcaa tcctgttttt aggttgaatg
    58681 tataagtacg gccatccaaa gaaatcttcc atgatttggc aagtcccgga tccaatgcaa
    58741 ttttgccggc acgttgaatg atcaaacctt caaaaagatt tcctactata atagaagatt
    58801 ccgtttcctc cacaaacgcc ggatcaagtg ttaccggggg accgttctta ccatagatca
    58861 ctgtaccgcc atattgcggt gtgtctccgg ttgcatcact tacagcatcg ttttttttac
    58921 aggccgttgt aaatacaagt gccaccgcta aaacgatctg aaataaagat cgttgattca
    58981 taaatacatc ctttcggtat gaatgatgta gtatggaaat agttttccaa tcacgctatg
    59041 ttaggatttg acccacacat tcttcagatt acgtgtataa ctagcatacg ggttaaaatt
    59101 cattacttca cgccgacgcg gaatcacaat ttgcgtatgt gcgatggtaa accaaggtac
    59161 atcacgttgg aatatttgac acgcgttttt atatagctga tcacgctctt ccgtttttgt
    59221 agctgcttta gctttaagca aaacgcgatg catttcgtcg ttatcataaa aagcgctgtt
    59281 tccggttcct ttttgacgca cggtggtttt ggataagaga ggataaaaga aattatcggg
    59341 atctggaata tcggcaatcc agcctccgat caacaaatcg taatcaccga gataggtatc
    59401 ctgaatatat gtctcccacg gcggagtaac tacctcgatc tcaataccta ctttttccaa
    59461 ttcactacga atcatttcaa cggctttttc cgggtgcggc atgtattctc tgggcaaagg
    59521 cacattccac aatttcagtt tgaggtttct tttatatccg gcttctttca gaagcctttc
    59581 tgattctgcc ggattatatg gtgttggacg aagatcgtca ttataaccga gcatacccgg
    59641 aggtatgggg ttttttgccg gcacaccaaa gctgccataa acggcttgaa ccagtttttc
    59701 acgattgatc gcataagcta cggcttttcg cacacgaatg tctgacatgg gttttttgct
    59761 catgttcatt gccaaaaaag agacattagc gccgggttgt ttaaattgaa taatttgctc
    59821 attgcgtttg actcgagcca acatgtcaaa cggcggcggt tcgatcaaat gaaattcgcc
    59881 ttgttccaaa cgcgtaattc gtttttccgg gtcgggctca ctcaaaaatc gtatggtgtc
    59941 caaataagga cgctcacccc aataattggg attggctaca agcgaaagat cattccaatc
    60001 gctggaagcg ctcatcatga aagcacctgt accgacggga tgtttttcaa aatccgcacc
    60061 atatttttcc actgccgcag gacttactat agccaaaaac tgcatactca gaattttcaa
    60121 aaaagtagca tccgcacggt gaagtttgaa ttgtaccgtt gaatcattga cggcgcggac
    60181 ttctcgaatg agttcatcca tattaaaatt gatccagtta gaaaactggt cagcggagcg
    60241 acgggcgccc tttttttggc gatccaaact atataaaact gctttggcat caaacggtgt
    60301 cccgtcgtga aaacgtacgc cgctttgaag attaaacgta tagcttaatc cgtctacggc
    60361 gatgatccac gatttggcca gccctggcac aacatccaca ttacccggtt ggaggcgcac
    60421 taaggtttca aaaatattat cggtcagaat agaggattcc gtctccgcaa ctttggccgg
    60481 atctagtgtg accggcggac catgtttacc gtagagtaat gtacctccaa agcgaggttg
    60541 attatcggaa acagtattta cgttttcttt tttggaacat ccgctcacaa aaaagaacat
    60601 aaacgataat atcagtaaca aaaaacggaa ggggatttta taattcggca tgttgtaagt
    60661 aattatgata tcggttgaag ttgaagttca aaaggcatga gttgagcagc gctatccatc
    60721 ttgatgcggg cgtattcgat gatttcctga ttttcgagcg actgctcttt tagaccgtct
    60781 gctgcgatga catacttatt cgtcgctaaa gcttctttga taaatgtcat tgaactgaat
    60841 gtttcagaca aaatactgct gagcgttatc aaataaaact gacggtacaa ttcggatttg
    60901 ccgagcgctt cgatttcatt tcgtgcactt ttgaggtaat caggaaattt cccgggttcg
    60961 atcgtatacg ttttttccgc ttcttctccg gtttccggat cgatcatcgg ttttccgttt
    61021 ttatcttttt tgatcacctt gacatcaatt tttccatacg taccggcaaa agccttttgt
    61081 aagatatcat gaagcggcaa tggttcacgt tgtgccgcta caccgagatc ggcacgcagc
    61141 ctttcgacaa gctgccgcac acccaactca acgcgcaact cattggagcg cagtttggtc
    61201 gacgaataac ttagtaaagc cacggcttca tcggacagtc cgagcgcttt ttgataaaac
    61261 gccgtttcat cttttatggt acgattgaca aaaccaagca gcagctctcg ggcgtctact
    61321 tgtttacgca gggtggacac gtctatgttt tcacgcgctt ctttgtattc gtattgttca
    61381 aggatacgat tgggatcaat attttttata ccgcgtaaaa gttccgtctg tgtcattttg
    61441 atcagcggac gtttaccttt acggcgcaat tcgtgcacct ccatatccca accggatgca
    61501 acaaactggg caacgagaat actccgcact ttttcatact taccataata gccagcggca
    61561 aactttcctt cgctttccag ttctcgtttt tcacgatctt cttcgttaga aacaacgtcg
    61621 agaaacttgt catcaatgat cttataaact ttctgtcgca attcggtgcg cgcaacatca
    61681 atctcttctt gcgtatgttc ccttttgtag atgtagtcaa agagaatctg atcatccaaa
    61741 aggcggagcg cttgagctaa ttcgcgatta ttgagatagt gatacaaaat gatccattgt
    61801 tcttcaaacg gacgactttt aagcggtgtt tgtttttcct tttcgggcac aactgcgttt
    61861 tgtttatcaa atccgttata atacaatgtc tcacgacccg tatgtgaacc gtcaaattca
    61921 taccgaagtt ttttgatttc ctgaaacaat gtcagacggc gcgtaaagct gtaggtcaaa
    61981 gaaagacggc ctgcaagtac tttgatcaat tgatccgaaa cgatgtcgcc cagttcgtgc
    62041 accaaaagtg cggccacata aaacgacatg agcgcggagg cgtgacgttt ttcagcggca
    62101 cgctcgataa acatttcgtg aataagaggg aagtccgtct gatccgtaag atacgcccgg
    62161 aaaagatcgt gaacacgttc aaacccttcc aaggacgccg tattgctcat catacgatta
    62221 aaaacaaagc gcacttcaag atcaggcacg cgcgcgcgat attgcgcagg aagattttgc
    62281 atcaccttgt cgctgttgat cgtctcaata tcaagtttga tttcccggca aaacatttca
    62341 acaaccgctt catccacttc gacgttattt ttgacggcgc caagcagttt tccgaacgcg
    62401 tacattacat aatgtttcat tttcggtgaa tacgagcgca caatgtccaa cgttttttca
    62461 aaaagcgata ccgttttcgt atcggtcatt attttgaaaa gacgacgttc gtcaaaataa
    62521 tagtagatca atccctcaag ctcttcgtac gcggtaagtt cacgcacaat ggcgttttgt
    62581 gcgttttcgc tcaacgattc gcctttttgc tgttgaacga tctgcaacgt tttttgaatt
    62641 tccgttttta aagatttatc ggcgtcttgt atttctttcg tcaattccgc attggcctga
    62701 agcgacttgg taaaatcata aagatcgggc agtacggttt cggattcgcc cacttctttg
    62761 agacgatcat ataaaaaatc tacgacgtag tctgaaaagc catatgtttc aatcgcatca
    62821 cgaataatag acttaaaaaa aacaggatac acatcggtat ccaaatctaa tttaccgccc
    62881 catccgtcat gcactttttt ataaccggcg agatatattt tatctgcaaa atgcgcaaag
    62941 tgatgcatca gattgaccat cgcaccgaca tcgttctttt tactgataaa tgcggcatag
    63001 agtgaaatca cacgcataaa acgatcaacg tcgtcatccg catcaagaaa cggttggaac
    63061 accgaaaaaa tggaacgcgc tacctgaact tgatcgtcaa taaagtggga tggcgatgct
    63121 aactcaattt ctttaccgcg aaaccaaaat tcatccacat cggaaacggc atgcccatgc
    63181 tgcaacatga gatcaaacac cttcatatac agcacgcgtt gggtgataaa aagcgaaggc
    63241 aacgaatcgt accctttgcg gatatttccg ctttcctcaa gcgtgatgag aatgttcatc
    63301 aacgctttga aagccggttt tgcattcgca tcgcttatac cgatgcgttc ggtaagctgc
    63361 gcgatataat cttcataaat ggttttgacc tgatcgtcaa caccgacggt caggatatgc
    63421 ttaatatcta tcatgtcaaa ctatcgcctt ccgtcatacg ttctgtttcg cggttgtata
    63481 tcgtatcaat catatcttta aaatttttag taacgacatg gcgtttgatc gaaagtttgg
    63541 gagtaagctc accgctttct atcgaaaatg ctttgggtaa taatgcaaac ttccgcactt
    63601 tttcataccg cgcaaatccg gctttatctt gcgcttcttg gatcaattta tctatcatgg
    63661 cgtatacttc tttgttttcc agcaattctt cttcgctgtc aaacttgata ccgtgctgca
    63721 tcgcccagtc gcggaggcgt tcgcgtttgg gaacgatcaa cgccgagata aaattacggc
    63781 gatcgccgat gacaagcgct tgctctacaa aaggaatggt gatcaacgtg ttttcaattg
    63841 gctgtggtgc aatgtttttt ccgccggatg ttacgatgat gtttttctta cgatccgtta
    63901 tgtgtaaatg attatcagag tcaagaaatc cgatatcgcc ggtgtgcaac catccatccg
    63961 agtctatcat ttcacgtgtg gcttcttcgt tcttgtaata ccccatcatg acgtgaggcc
    64021 cgcgtgtcaa tacttcgcca tcctgtgcga tacgcacttc gacaccgtca atagccggac
    64081 cgacggctcc aaattttact ttttcaggac gattgacggt gattaccgga gaagtttcgg
    64141 ttaacccgta cccttcgagg atcatcacgc ccatataagc aaaaaactca gcaatttctt
    64201 tggccaaagg agcgccgccc gacacaaaaa atcgtaagcg cccgccaagc cgatgatgca
    64261 gctttgaaaa aacaagtttt tccgcgatct tgtatttgaa tccgcgttca ccggtttcat
    64321 aagccgattt acctacaccg agcgcccatt caaaaatttt gcgacgaata gggggcgccg
    64381 tagatagtcc ctcttttaca cggtcgtaca ttttttcgta taaccgaggt acggcggtca
    64441 ttacggtcgg attgatttct cccatgtttt gcggcacctt ttcgatgctt tccgcataag
    64501 caatggcgca accgcgaatc accggcataa agtgtccggc catacgttca aaactgtgcg
    64561 cgagtggtaa aaacgacaac atggtatcgg tttcgccgac ggtagccact ttaacagccg
    64621 ctagactatt actcaaaata ttattgtgcg agagcatcac acctttgggc atacccgttg
    64681 taccggatgt ataaatgatc gttgccaatc ctttgggatc tatgctgtcg atttgttttt
    64741 cgatatatcc ggggttttca gtgccaaatt tttttcctaa ctcctgaagc tctgaaaaaa
    64801 acaaaacgta gtctttgtgt ttatcttcgt gagttataag cacggcgtta ttcaacatcg
    64861 ggcactgatt tttgcactca atgattttcg caagctgctc acgatccgat gcaaccacta
    64921 aacgtgcatc cgaattttgc aggatgtatt cgacctgcgg cgtaggaaga ttggggtaaa
    64981 tcggaaccgt tacggcgccg agtgacaaaa tcgcaaaatc ggacatagcc cactcttcct
    65041 gagtcggaca gagaatggct actttatcat ctttcttaat tcccagtgat gccataccca
    65101 gcgccagatt gcagactatt tcgtgcacct gtccatacgt caaagcgcgg tacttgccat
    65161 cacgtttgct catatacagc gttttatgac caaaacgacg cgcactctgg tgaaatacat
    65221 ccgccagatt tctgggtgaa ccgttatcca tgtcggtatc tccaaagaaa ggttgtgagt
    65281 ataggcgtga aaccgatgcg tttgtaaaag caacaccgga tgaaacgtca gaatggaaca
    65341 gtgaaaaatt agacaatgct cataataaaa gcaaaggatt tggagctcaa aaaggtttga
    65401 ccttacagtt cgatcaaacg atgatcccaa gagtccaaag tgatttgctc actgatcgta
    65461 tcaaacaaag taaagccgta tttattcaaa taatataaaa tcgaaagctc acgctcctga
    65521 aagttatcat tgggacgaat atgcagcatg gctttcgtca gttggccatg ggtcgtcaga
    65581 gcggcgcgtt tttcggcatc gcgtgttttt tgtgtaagca cctgcatcgc ttgttgcatc
    65641 ttatccatcg ccgtagcaga cgcacctttg agtgaagcat ctatcgccgt caattgcgtt
    65701 tcaaggtttt tcataccgtc ttgcacttcg ttcataaacc tcagaagcgc cggttcaagc
    65761 gaaacattgg attgttgatt aattcgttcg atcaccgctt cgccgtcatt gatgatgtcc
    65821 gctatcgtta tttgatattt atcgagcact tttttgattt ttttctcaac taaagtaaaa
    65881 aatggcctgg gaattatgac cggcgatgcc atatccatgt gagtgtacaa atcccgaaac
    65941 tgtgcaaagt aggcgatttc gccaggaccg gcgacataaa aaacggttgg taataacgta
    66001 tcttgaatca atggtctgag aataacattc ggaataaatc gttgcggctc attctccagt
    66061 atatgaagaa tatcattttt gtatgttttc cagatgtccc gtgtaatacg gattttgtcg
    66121 tgccctttgt tattaaacga aagtttattc tcgccatagt tcgtcaaaaa aacataactc
    66181 ggttcggttt caacttgagc gtgataaccg gcaactttta acgcattgct ttgatttaga
    66241 accgcttcat gtaatgattc ggattctttg agggctcgtt caaaaacagg ttttactatg
    66301 cctttaaaac gtacatccga aggatccatt agaaccaatc ccatcggact aagccatgat
    66361 gataacagca ttccaaaagc ctccgcccaa gattttccgg gttggtagga ttttgtaatc
    66421 gtatcaaata acgcctgtga aaaatcggta ggattgagcg ctgttttcag ttcttgtatt
    66481 attcgcgata tgtcatccgt caacgcaaac cggtgcaccg gagttttgtt ctgattttca
    66541 gaaatgccgc catcataaaa aattttgaca agctcgtttt gaatgtttat aagctgtgtt
    66601 gagcgtactt cttcaaaatc atgatcttca agttccaacc aaaacaccgg tataaaatta
    66661 tagtcaggaa attttgtttt gagcaatcgg caatatttta ccgtacctag tgctttaaga
    66721 accgtatata tcggaccccc caaaagcccc gtttgttgac cggtgacaac tgcgacggta
    66781 ttcggctgac gaagcaacgc aacattttcc aaagcttgtt ttgccgattg gaaacgatgt
    66841 tgctgatcgg caagaatgtc cgatagaagt tcacggtttg tcttatattt ttttaaaact
    66901 tcgctgaacg tatcggacca tgctttttga tcggatgggt tagcggaaaa aaacgaagtc
    66961 gttttttcgg gtgaataaag ataatccgta aataacgatg ttgtgttctt gagttcgcga
    67021 aaggaaatag tcatgctgtc atcctgttgt attgctatag gctacacgat tacgtccgcc
    67081 tgatttggcg cggtaaagct gttcatccgc acgttgtatg agttcgtcta tggtttgcgc
    67141 ttcgtcggga aatgtggcca cgcccatact gaccgtaata tcacccatgg gctgtgtttg
    67201 ttcatacggg aaatcttcat cttgaattgc cgcgcgcaat ttttcggcaa atttcacagc
    67261 ggcgaccttt ggcgtttctg gtaatacgat gacaaattct tcgccgccgt agcgtgcgat
    67321 gatatcgctg gtacgtgtga tatttttaaa aacaacggca agtcgtttta atatctcatc
    67381 gccggcgggg tgaccgtggc gatcattgta tgatttgaaa tgatctacgt ctatcatgat
    67441 catggacagt tttgaccaat gacgtttggc acgccggatt tcacgctcca gttggtgatg
    67501 aaaatagcga taattaaaaa catctgtcat cgcatcagtg atggcttgtt tttcggcaag
    67561 catataggct tcacgtacat cttcatacaa ctgaacattg tctaaccgaa gaccgacggt
    67621 cgtagtaaag gaatgaataa tctgccgaaa cgcatcgctc atacgtccat ccggaatacc
    67681 cagcgctaaa acacctttga gatcataaaa acaacaatta aaaaaatatc tgctcgcatc
    67741 acgaaggcgg gaagggtaat caagatcacg ccggttgtat tgatgaatca aatttcgcca
    67801 ccactgtccg tcttttttta caaacgcttc acaatcctga ttttcacact caaaatcatc
    67861 cgctgcgcgg gctcgaccgc gaatgggaac aaaacaaacg gtctgtgcgt gaaaagattc
    67921 gaaaagtacc tctaaacggc gcgcatgcct ttcgttttcc cgttgcaatg cgatgagttt
    67981 atgaacatct atggttaccg attgattgtc ataaaccgtc tcaaaaaaag gaggttctgt
    68041 aaaagggatg tgttctgttt cagggagaaa atcatatttg taactggaaa aaacatcgag
    68101 cgttaaatct ttttcgttaa ccacatatat cgcggcaccg gccagagaaa gttgatcgct
    68161 gataagctga ggcatttcac ggtaaatcgt cgagcgatca cggtaggcca tgatttgcga
    68221 tgtgagttga ttgatctgca tcagatcggc attattggtg cgaacttcct gtatgagacg
    68281 atcgagagta ttattggttt tttgaagttc tttgttggcg taggagatct cgttgatttt
    68341 atccgtcagg cgatgaacca gacgcagtgt aaagcgtcgc aactcaatat tttccgattc
    68401 gagactaaca ttcttatttg tatgtgtctc agaatcaaag caaccgcgga tcagatcaga
    68461 aagagcttcg tattcaatcg gcttaaccaa aaaaccgtcg catccggcca tggttgcgag
    68521 ttgttgatcg tcttcattcg gtgttgcggt agtagcgatg ataggtgtat gcttgagcgt
    68581 ttcggaactg cgcatcttga tcgtgagttc aaatccgtcc aaacccggca acaaaagatc
    68641 tatcaaaata agatcgggac gttcggcaaa cgctttggcc aaaccggact cgccggtcgt
    68701 cgctgttata atcgtatgac ccatgcgctg aataagctgc gtcatgtatg tcatggcgac
    68761 gggatcgtct tcgatataca gtattttttt catgcctgag acgtccgatt atccttacac
    68821 ttcttccacg ctaacggttg tttcaatgag atgtttaggt atcaggcacg gaaaaaatgc
    68881 cacgatttcc tgtggctttg gacgccctcc tgcaaaaccg gttactcccg gaggcccgct
    68941 ggtaaccagg ggcgcaattt ccttgccgaa gcgatctacc tttttctgat ctttatcttt
    69001 tacaccgaca cgcaaaacca cttcgttgat gggattgggt atggcgacca tgccttcgtg
    69061 acacgtgttt acgccgagaa attcggtgtg tgtttcttcg tattccattc ctgcccgcgt
    69121 aagacgtttc cataaaactt ctgcggccaa cttagctttg ggcaacgcat cagggccgga
    69181 aatcgtaagt tgtcctgttg ctttatatcc tttgagataa ctggcgctga ctttgtaaaa
    69241 ctccgttgaa ggtttacctt gtacaccgaa tactttaaca cgatcttttc cggcttgctc
    69301 cagacgaatg gatgtaaaat cagcgatgac gtccggcgtg atatagttat gcgggtcgcc
    69361 catttcgtac accaactgtt ctgaaacggt gtccaccgtc accatcccgc ccgtgttttc
    69421 atgtttactg acataaaatg ttccgtcagg ataggcctcc acgacgggat aaccgatatt
    69481 ccacatatct ggcacatcct gccaccgcgt aaaattacca cctgtacatt gcgcaccgca
    69541 ttcgatgata tgacctgcaa ctgttcctgc ggccaattta ttccaatcat caaacgacca
    69601 gccgaattca tatatcattg gcgccattgc caatccggta tctgtagttc gtcctgcgac
    69661 aacgatctgc gcgccttttt gtaaagcttc gactaaagga agagaactca tatacacatt
    69721 ggcgcttaag atgtcgcggc cttgtttcat cagatcatac aaactttcac cggtgtccat
    69781 ggacttcatc ggaatttgtt tagtttccat ttcactgata cggggaagaa tattatcacc
    69841 atagacgacg ccgatcttca gacctttgat acctaatttt ttggccacgg caaaaagggc
    69901 ctgacgacat gcttcaggat ttacgccgcc ggcattggcg atcactttaa tgtttttttc
    69961 tacgatcgtc ggcaatatgc gttcgataag ttgcacaaaa tcggtagcgt aacccagctc
    70021 cggttttttg agtttttgac gttgcatgat ggacattgtg acttcggcaa gataatccaa
    70081 agtgagataa tccaattggc cgccttggac taaatgcaca ggcgcatcaa tgctgtcgcc
    70141 ccaaaatccc tgaccgttgg ctataaaaat tttgtctttc attttaagct ttttatttat
    70201 gaggttgtta caaataatca attgatgaaa ctacgaaaac aaattaagct tttaaatgaa
    70261 attctgtggt ttctgctata cttatacagc ggttatccgg cggattgggt atcaagtagt
    70321 ttctttatgg ctttggataa ttcttcgacc gtatatggtt tattgactat atggtcggga
    70381 tatttactgt ggagcaattg taaacgatcc gctccgatat atccggatgt gaaaaggaat
    70441 tttaattccg gtttaatttc gagcaatttt tccaatgttt cgatgccatc catgccgggc
    70501 atttgcacgt cgagtattac gagatcaacc ggtttttggt tcatgacttc aacggcgcga
    70561 tacccgtttt ccgccgtata acattgatac cctagacgct tcaaaaccat ttctcccatt
    70621 tccaatatgg gtttttcatc atccactagc agtatggtgc cggtgccggt ttcatattgc
    70681 ggtttttgcg cctgcacatg gggtatcgta acgcgctggg aaaccgggaa accaagctcc
    70741 atgcgcgttc ctacaccgat ttgcgaatga acatgaatat atcctccgtg attggtaatg
    70801 attgttttga cgacagccag ccccaatccg gttcctttgc ctatatcttt ggtcgagaaa
    70861 aacggttcga aaatcttatc cattacttct ggaggaatgc ccgtgcccga atcggctatg
    70921 gatagttgta cataaagacc ttccggcaca ttatattttg ccgattctgt atcatccagt
    70981 tcaaccattt tggtttcgat accgatactc cctttattat tcatcgcatc gcgggcgttg
    71041 acacacagat tgatgatgat ttgctgtacc tgtgttgcat cggcatctat ataaggaatc
    71101 tcgggttcta agtgtaaggt gatcgtaata tttttgccaa gaaaccgatc cagcatgttt
    71161 cgggtttcat caaccagatt attgatgttg agcaatgctg cactggacgg agtttggcga
    71221 gcaaaaacca aaagttgctt gacaatatct gacgcacgac gcgctgatga cacgatcacg
    71281 tcggcgtgct gatgaacagt atgcttatca taattttggt taagcaacag ttcggctgta
    71341 ggtaaaatca cagccagcat attattgatg tcatgagcga taccgccggc aacacggcca
    71401 agactctccg ttttttgagc ctgaatcaga ttttgttcta gttttttccg ctccgtcata
    71461 tcggtcatcg agcccaatac gcgagttggc cgaccttgct catctctcaa tatgtaagct
    71521 cgatcataaa agtaaccgta acttccgtct ggcaaacgaa agcggaactc atcgctccag
    71581 aaattctgcg tactttctaa aacgtttttg tatttttggg ttacacgttc acggtcttcc
    71641 ggatgcagtc gctcttgcca aagccggtaa ttgtcctgcg ggtaatattc ttctacggca
    71701 aacagcttac gggcggattc attccaccat actttatcct gccgtgcatc ccaatcccaa
    71761 accgcatcat tggtcgctcg agcgatgatt tcaaaacgct cgacgttttc cttaatagat
    71821 ttttctgcct ttttacgttc gtctatatta gtcatcgtgc ccagcatgcg gtaaaccgtg
    71881 tttttttcat cacggagaaa aagaccgttg tcatccataa acacgtaacc accgtctttt
    71941 tttcgaaagc ggtattcgac gtgataccga ttaccagaag ccatggcttc atctaaaaga
    72001 gaaatggcgt taggcctgtc ttcgggatga atcattgttt cccatgtttt cagatcaacc
    72061 cgtaaaaact cctccttggt aaatcctgtg ttgatttcga tcgcgccggt ccaggtgatc
    72121 gcaccggtcg ttacatcaag atcataaatc aactgacctg tctgctcagc tatgatacga
    72181 taacgttctt ctttttctgc taacgcacgc tccgtttcac gacgactcag aagctcttgc
    72241 cgcatgatac taaaaagcaa agtgatcaga agtgttgcaa ataactccgg agtcatatta
    72301 acaaaaaaag cagaagtgat gtgcatggcc ggcatcggat tgggatcaaa aagaagatac
    72361 aatccgacaa tctgcggtaa caggaagaga taatagtata gaatcacaac catcacccac
    72421 aagatgggtt cttgacgtga tttggtgaat cgcgaaaaaa aacgatgata aacaagccac
    72481 gccattgcag ccgaaaagcc atgtgaaaca gccgaaaata attggagttc gttggctgca
    72541 ttcactgccg tcatcgcacc ggtgatgatg acaaatcccc aatgcggaag gaataaaata
    72601 ctgatgagta tggcaacctc attgaggctg ctggatgtca taaagttgaa cgtgggaata
    72661 acaaagcgga tatagctcat gccaatggac aaaagcccaa agccaacagc aatcagagtg
    72721 tttcgaataa aaaaacgctt gtcatgtttc accgctatca tcacaactcc ccataggtga
    72781 acaattgcaa agccggaacg ttattttata aatcaagcgt atcggaattc aactaaatga
    72841 gaaaccacag agatcaaaca aaacgaaaac gcttttacga ctggagtcga attttttgag
    72901 tgacgtcttc gtctttttcg taatattcgt atgtcttttt ttcttcgatc acagcgccca
    72961 tagattcaaa aagtttggat agatcttcgc acgcgccgcc tttatagcct ttgatcgcat
    73021 attccagcga gccgtcgcga gttattgtaa aaacgatttc tttatagtcg gatgtcatag
    73081 cgccccctac ccgttccatt ttcttaaaac caaccgcacc gattgatctt cgttgactga
    73141 ttcttttacc aactgaaatc cggctttttg cgcttctgaa acaacacgac tgtaggcata
    73201 tttctgtgtc agtttaccga caaaatcttt atccgaagcc aaaaccatat catcccccgc
    73261 aatctcaaat tcaccgctac ggttttttat aaaaccgatc attccaaatg ctgtttttgc
    73321 cgttagatcg acatcgtacg aacgccctaa aagggttttt atttttttgg aattagaaat
    73381 ttcgcaaccc atttctttca gagcattttc caagtggaca cgctcgcgaa gtgcggtttt
    73441 cagcgaaacg aacttagaca tgaaattact ttcgtttggt aacgcgacag tttaacttga
    73501 cgcaaagtag agaatttttt ttcgtcttgc aatctcaaaa aatcgaggcg agttgaagtt
    73561 atgcgtaaat ggattattgt ttttttgagt ttggcttgtt tgttttttta tcttaaaaac
    73621 tatgacatct catcggttga aggccgctcg gaaaaccacg gcgtcataat caaaatcagc
    73681 gatggtgata catttttgat cgaagtgaac gggaaaacgg aaaaagtgcg tttgatcgga
    73741 attgacacgc ccgaatccaa ggataatctt aaagccaaaa aagacgcggc tcgtaattct
    73801 gctgatcttg atgcgatggt tcggcaggga atcgaagcac gccgttttgt ggaacggaat
    73861 tttcctctcg gaacaatcct ccgcatcgaa accgatcgcg atacgcgaga tcgttacggc
    73921 cgaatgctcg cttatctttt tacggaagac gatacgatga tcaatgagac catcatccgt
    73981 gagggttacg ccacaccgtt attttattcg cctaactaca aatacaaatc gcgtcttgag
    74041 gaagcatacc gttatgccaa atcaaacaaa aaaggccttt ggcaaaatgg caaaccgctg
    74101 catattcgaa aagacgatta agttcatata agactatttt tttatagatt ttctgctttg
    74161 catcccatac tttgccgcgg tttttttaag gtaatcgaaa attaaaaata tcatataagg
    74221 atttgctata tgccacttcg ttgcggtatc gtcggtctcc ccaatgtggg taaatcaacc
    74281 atttttaatg cacttactgc agccggtgct ctggcggcaa actatccttt tgcaacgatc
    74341 gaacccaatg tcggcgtagt gacagtgcca gaccatcgta ttgacgctct gatcaaagtc
    74401 gtcaaaccca aacgtgtact ttatacgact gtgaattttg ttgatatcgc aggtcttgtc
    74461 aaaggcgcca atgccgggga aggattgggc aatcagtttc ttagccacat tcgtgaagtg
    74521 gatgctatcg gccacgtcgt acgttgtttt gacaacgcgg atgttgtcca cgtcgaaggc
    74581 agtgttaacc cggaacgtga tatagccatc attgataccg agcttgtact caaggatctc
    74641 gaatccgtaa acaaacgcat ttcccaagtt gaacgaactg caaaaagcgg tgataaggat
    74701 gcgaaacttg cttttgatgt gctgagtaaa gtcaaagtac aactcaatga cggaaaaccg
    74761 gttcgcctga tgagtttatc cgaaaatgac cgcaaagcta tcgcggatct ttttttgctt
    74821 accgacaaac ccgtgcttta tatatgcaat gtatcggaga gcgatgtact cacggaaaat
    74881 aattatgtca aacaagttcg ctccattgcc gaaaaagaaa atgcacgtgt tgttgtgatt
    74941 tcggggcaga tcgaatccga aattcagcaa ttacctgaag aagaacgtaa gggatttctc
    75001 gaaagtttag gtctcaagga accggggctt catgcgctta tccgtgaaac atattcgtta
    75061 ttggatatga tttcttttct taccgccggc gtcgaagaag tgcgtgcatg gacgatcaca
    75121 cgcggcacta aaaatcctca ggccgccgga gtaattcatt cggatttcga aaaaggcttt
    75181 atatgcgcgg atgtgatttg gtgggaagat tttgtcaaac acggaggcga acaagcctgt
    75241 aaagacaaag gtctgctccg taccgaagga aaagaatacg tcgtcaaaga cggtgacgta
    75301 atgcacttta agtttaatgt gtaagaatag tcaataaatc ttaaacctag aaaactacag
    75361 cattttccag agtgttttga ccaatttttc taaccccgtt tgggcaaccg aggatatcat
    75421 caatacgggt gtgccatctt tcattttggt tcgtttgggc aaagtatcta ccgtatctat
    75481 tttggtcaat acgacaaggc gaggacgatt gagcaattct ttattaaaag ccttcagttc
    75541 cttttttaac gtcacataat ctttttcggg gtttgcgttt tgaacatcaa tcagaaaagc
    75601 aagcgcttta gtacgttcaa tgtgcttcaa aaactgaaca cccaaacctt taccctcatg
    75661 agcgccttcg atcaagcctg gaatatcagc caatactaat gagcgatatt cgccgatctt
    75721 tataatacct agattcggcg tcaatgttgt aaagggatag ttcgcaattt taggtttggc
    75781 cgctgttact gacgcaagca aagtagattt cccggcattg ggaaaaccga caagtcctac
    75841 atccgccaat actttaagtt caagatccaa aaatttttct tcaccgcgtt caccgggcgt
    75901 aaattcgcgc ggcgtgcgat tggtaggcga cttaaaacgt gaatttccct taccgccttg
    75961 accgcctttg gcgagaactg cacgttgtct gttttcggta agatcacaca gcatttcacc
    76021 ggtttcttga tccttgatga tcgtcccgac tggtactttg attacaatat catccgcaaa
    76081 acgaccggac atattttggc tgccgccttt ttgtccgtgc tgggcacgaa aaatgcgatg
    76141 atgaacgatg tcttgaagcg tatgaagatt ttcatcggct tcaaaaacta catcgccgcc
    76201 tttgccgccg tcaccgccgt ccgggccgcc tttgggaata aatttttccc tgcgaaaact
    76261 tacacagccg tcgccgccat gaccggcccg cacttgtatt tgtacaaaat caatgaacat
    76321 tttttgagtc gcttaagcca attaaattat ttcgtatctt taggaggatc aaaatgacgg
    76381 cggcatcgcg cttcgtacgt acctgtcgca cctaccaaaa cgcgatcggc gctttggaca
    76441 agtcgctgtg tatagttggc cggtgcacca cataccatac agatagccat gggtttgtct
    76501 accacttcag ccaccgcaag aagctgcggc atcggctcaa acggccggcc tagataatct
    76561 ttgtccaatc cggcaacaat cacacgttta cccatgtatg ctaatttatt acatatttca
    76621 acaatcgaag catcaaaaaa ctgggcttcg tcaacgccaa cgacgtccgt tctaggatca
    76681 agtttctcca tgatttcgga cgctttttcg ataacttccg aaggcatttt aagttcgctg
    76741 tgagacacaa tatgatcttc actataacgc gaatcaattt gcggtttaaa aacctgaaca
    76801 cgcattttgg caatttctgc ccggcgcatg cggcgaataa gttcttcaga tttcccactg
    76861 aacatgcttc cggctatcac ttcgatccaa ccgctgttat gtacaatatt catatatgaa
    76921 acgggttaaa ttaatgctct gtaaactaca aattatttac atccatcagc gcggcataat
    76981 aatgttattt tggtgtttcc gcaaattgtt tttattaagt tatgtacaaa gaaatatatg
    77041 gagtctatga accaacaaat aactatcaac ggcgtaggca ttcacagtgg gaaaccaagc
    77101 cgtattctta ttcgcccgca tgaacacggt gtacttttta atgttgaggg aacgccaatt
    77161 cctgcgctta tccaacatgt aaaagacacc caacgcgcta ctacgatttc tgtcggcgat
    77221 cgttcgttac gtaccataga gcacctctgc tcagccatct acggattaaa tctcagcggt
    77281 gctgaaatca ccgtaaccgg cggtgaaatt cccatcttgg atggaagcgc aatgcctttt
    77341 atcgaagcgc tcgctgacac tcttactact gaaagccaaa atgaagcact aaccattgat
    77401 cgtaccatca ctcatcgcaa ccctgacaat ggagcgttta ttcgaatcga accaagccaa
    77461 aattttcatg tcacttatac tcagcgtttt caaaaacgtg ccattgatac tttttctttt
    77521 tttagttcac ctaaaaatta catcaaagaa atcgcgtcag cccgtacatt tgcttacgcg
    77581 ggtgaattac aattactata tcgtaaggga tttattcgcg gtttggatcc taacgccggt
    77641 tttataattt tggattccga acccaattgg gacttgatgg aagaaattat cggtgaagat
    77701 gcacgtagtg cggttcatga aacgcccttt tataagatat tatcaaaaat tcctccgcgc
    77761 tatcccaacg aggccacgcg tcataaagtg ctcgatattt taggtgattt ttcacttctc
    77821 ggtatgccta tcaaaggttt gataaccgcc gatggtactg gtcatgcaga gaatatagct
    77881 ctcgtccaaa aaatcgcaga aacctacgga ttgattcatt ttgacccgct tggggatgac
    77941 gaataagttt tgaataaaaa agccgacgca aaatacgtcg gcttttgaat taacatgata
    78001 ccggagaggg ttttataatt tcaacagact aaacatacag ccttcgatgt cttgttttaa
    78061 gttttcgtga ttttccggat aataatatcc ttcacacata cctgtaaaaa catagaccat
    78121 agtaatgtca ccattggtga tcgtaccacc gccggtttta ttttgtaaat caaagacaaa
    78181 tgagcctgtg gatgataaag cggcatcggt tttggtgatg gtaaatgtca gtgttacact
    78241 ggaaccgtca cgaatcagag taaactggga ggtcgaaccg tcactttctg tgatcgtacc
    78301 cgtggtttca aaattataac gttttcctgt taccgttcca ttgagtgttc tcccccggct
    78361 atccgaaccc gtaatagtat acgataaacc ctccggcgta ccattgccgg taggcgctga
    78421 agttcgcgtg atagtcaata ccgtagaata tgtcgtacca tcaaggaagt agaatgttga
    78481 cgaatcggtt gataccgctc cactcatgat tacttttgtg attttacgat aaaggctatc
    78541 gccggccggg aaatctgtct gaatttggat ggttccgctc ttagaaagat tgctgctatc
    78601 ggccgtgata ttcatcgttg aaaccgaata ccggtgtgta ccgtcacgaa gtgttatatc
    78661 catagttccg ctcagcgttc gctcgcccgt tgtagcattg acagtacggg tatatgttat
    78721 ctcggatgta cttgatgatg catcggtata attagtggtt gtttccttat gggaagtgcc
    78781 tccaaccgga tcaatagaaa tgcttgtaac tcgattggat atgcctgaca ccgtcgcaaa
    78841 accggttgtt gtagcagaat aacttagttt tctgtcggca aacttcacgc gtccgacatt
    78901 acgaattatt gtcccgttcg taatctgtgt aaacccgggg tattcctcat acgttttgat
    78961 aatcgtagaa tccttaaaac ttgatgtcat cacagtattc tgagtaatga tcacattagg
    79021 gtcgtatgta aatgtcgcac gataaaattt cttctcaaca cgactgctgt ttgggaaatt
    79081 tttggttgct acgtcaatac ttgttaatct tagtgtcgtt ggatcaaaca cacgtatgat
    79141 cgtagcttgc gtgccatccg gataattacg tgtgatggta tcacgaccca cgaacgtaaa
    79201 tgacgaagaa cttttcataa gatgttcgtc agatatatct tttattatcg aactcgctgt
    79261 atagtcggat tcatcaccgg cgccgttata tccattttcg gaagttattt cctcattgtc
    79321 cgaaaactcg tcatccactt gactgaccaa cgctgttgtt ttcaccgccg cttcaacaac
    79381 ctctttttgg tcatcggtta gtgtgccatc acttttttct ttttcacacg caatcataaa
    79441 aaccaatagc agcaatgtcc ataatcgttt catactttag aaaactcctt atttatttaa
    79501 atactgtttt aatttggtcg ctaataatta cctacacgtt tatgcaggct ttgtgccaat
    79561 ctataaaaaa ctgttattat ttagtttaac tttaaaaaat aattttcaaa aatctacttt
    79621 gacggattta ttgccatttt attttgacgc tttgtaacga ttttaaccac acatatagta
    79681 atgtttattc ttgtattttc tttgatgaac ctttagcttt ctaaaaaaag cataccctac
    79741 ttatgcaaaa cgatctcgaa gtttacatac aagcgcgcta ccctttgatt tatttggtct
    79801 catgggagga ggagcgcatc ctcaaaacgc tcgaccagat cgctattcat cttcgcaaaa
    79861 tgctttttgt ttggacgcaa acacagggct tgtacaatta tgtgttgcca gaccatttgg
    79921 acgaatccaa atccgatcct gaggtgttgc ttaaccatat cgcagcttct caagataacg
    79981 gcatttttgt tttgtttgat tttcacgcgt ttatcgaaaa ccaccgtatt cggcgccaat
    80041 tgcgcgatct ttccaagtta cttcgcaaaa gcaataaaac gattattatc gttgcaccgc
    80101 aaatgacgat tcccctcgag ctatccaaag atatcgcggt tatggatttt gatctgccgg
    80161 gtttaacgga actcaatatc gcttttcaaa acgcacttaa ggttctccat aagcgccgcg
    80221 acgtagtcct agaattgaca ccgcagattt ctgaaaaact cttgaaagcc gcacaaggtt
    80281 taaccttggt agaatttgaa aatgtactgg ctaaatcggt agtacacagc aaagcgatca
    80341 acgctcatac gatccaggaa gttttggaag agaaaaagca gatcattagg aaatcaagca
    80401 ccctagaata tttttctccg gatgaaaatt ttgaacaagt cggcggactt gaaaacctca
    80461 aacaatggct taccaaacgc gcggaggctt ttactgaacg tgccgcccaa ttcggattgc
    80521 ctacaccgaa ggggcttttg ttggttggta cccaaggttg cggaaaaagc ctcacggcta
    80581 aagccgtagc agcacattgg aatctgccgt tgctcaaact ggatgtgggc agtgttttta
    80641 gcggtattgt cggatcgtca gaagccaata tgcgtcatgc catcaaagta gccgaatcca
    80701 tcagtccttg tattttatgg attgacgaac tcgaaaaagg attttcaggt atggcaagtt
    80761 ccaatttttc tgatggtggc acagccgcga gagtattcgg ttcttttacc acctggcttc
    80821 aagaaaaaaa gaaacctgtt tttgtaattg ctacgtctaa cgatataact cttcttcctc
    80881 cggaaatact tcgtaaaggc cgttttgatg aaatcttttt tgtggattta ccggaacaag
    80941 tagaacggga agctattttt actattcatc ttcagaagcg ccaccgtact acggattcgt
    81001 ttgacgtcgt aaagttagcc ttgctgtccg aaggatatag cggtgccgag atcgaacaag
    81061 ctatcattgc cggtttgtac gatgcctttg aggctggacg tgaattgaca accgacgata
    81121 tttccaataa cctgcgggcc acggtgccgc tctcgcgtgt catggccgaa cagatttcgg
    81181 cattacgcca atgggctgaa aaacgcgccc gtaaagcatc gcgaacacgt accgcaggaa
    81241 caggtattga cttggatcgc tttagtgtca actaactata ttttatttat cccggtaata
    81301 tcatgatcat tgaaacttta tctgaatttt ttgaacgcga tttgctgaaa ctcaaagaag
    81361 aaatcgcaag ttacagcaac gaaacagcga tctggaagat cgaaggcgat atccgaaata
    81421 cggccgggaa tcttgcgctg catctagtcg gtaatatcaa tcatttcatt ggtgctgtac
    81481 tcggaaaaac aggatacatt cgcgaaaggg aacgtgaatt ttctgattcc gatgttccac
    81541 gcataaaact aatacaggac atagatacaa cgattgcaat ggcgaagaca acgttttccc
    81601 gtatggatga ctccgtgctg tatcaaaatt atccgattga gaagcatggc aaaacagtaa
    81661 ctgtcgaaca catgctcttg cacctgcttg ctcatttaaa ctaccatcta ggacaagtaa
    81721 actatcatcg tcgcctcatc ggctaacatt gtttcgaagc ttacccgcct caattgtgca
    81781 aatatctaac agcgattttg atgttttata atggtatcga agaaatcaaa atctcataaa
    81841 aatgatttaa ctacttcgtc atggaacttc gcattattaa acgcgtaaca gaagacaata
    81901 accttatgat tgtcaagaca tgaaatgact tgtatcggct ttctaagaag tagtggtttt
    81961 taactccgca caaaatttgt atttttttgc ttcatacatt atcaccggcg cacacagaat
    82021 cttaaatatc agaattttta tacacaggaa acattctaat gggtttcaca ttttacatat
    82081 tcttgacacg attacaggaa actgaacgat gaaagagatg caaacaggtc agttttttgg
    82141 tgaaactgac aaaattatgc atctggacgg tattacgctt accaaaacgg tttataccca
    82201 tgattatgta gattggcatt accaccaaaa tgcttacttc acattccttc ttcgtggcgg
    82261 tctgatcgaa accgatcgaa aaaaaaccgt gaggtgtgct cccggtgatt tattgtttca
    82321 ttattggcaa gagtcacact gtaacattaa acctcctgaa ttcactcaag ggttccatat
    82381 cgaaatcgag cagtcttggt ttgacaatct ggaactggat cgtcgcatgt ttgaaggaag
    82441 ttttcagatt accgatcctg cagttcgtat cgcattctat tctatggcac gcgaactcgg
    82501 ttgcccggat acggcttctg tagtttctat agaagattcg ctttgccgtg ctcttgtgca
    82561 tgtatctaaa actacagctt ttcactcaaa taagcctttg tgggtaacgc aaatacgaga
    82621 tgttttgcat gcccgcccat ttgacacgca tacattgctt ggcctggcca tgactctgca
    82681 tatacacccg gtacatttat cgcgcgcctt cactcaatat ttcggtgttc cgctgggtac
    82741 atatattcgc caaatacgtc tgcaaaaatc catcgaacgg atgtatgatc cttctttgtc
    82801 acttactaat attgcgttgc aatgcggttt ttctgaccaa agccacttcg ttcgttgttt
    82861 caaagctgtt accggaataa cgccgcttcg ctatcgccga gagttgtgca agatcaatga
    82921 accatgttaa atacgttcta tttctaagcc tccgcacgtg ttatattatg gtaacactaa
    82981 aataaaacat agaggggcat tatgaaaatt ttcgtaattc tatgcatggt cctttgtacg
    83041 agtattcctt cggtttctca ggttcctata gatcggcacg acggtatcaa agtttccgac
    83101 gcttcttcga tgcatatact accgaccact ttgctcaaat tggatagcgc tgcaaatcag
    83161 ggtgttttaa aaaacattac gagtattttg atcgcacgcc atggaacgct tgtttttgaa
    83221 aattattata acggcgccga cgaagctacg cttcacaata cacgcagcgc taccaaaact
    83281 atcacagcaa tacttaccgg tttagcgata gatcagcaat ttattccttc agaaaaaacg
    83341 cctgtatttc cgtatttcag agacaaaaag gaaatcctga atcccgatcc gcgtaaaaat
    83401 caaattacca tagaagattt gcttaccatg agctctttgc tcgaatgcga tgacgataat
    83461 cagttctcga acggaaacga agagcgcatg tatcttattg aggactacat ccagttcgct
    83521 ctggatttgc cgattcgggg gtttgcggca tggaatccca aacctcagga ctcgcctttc
    83581 ggacgaagct ttagttattg tacagccggt gtaattttgc tcggaggtgt attggaacgt
    83641 agctccaata tgcgtgttga cgcattcgca aaaaaatatt tgttcgatcc gttggggatt
    83701 cagcaaaccg cgtggcaaat cacgcctacg ggaatgccta tgacgggcgg tgggcttggt
    83761 ttacgaagcc gcgatttact caaaatcgct caactttatg aaaacaaagg tatatggaat
    83821 gggaagcgta tcatttcggg atcgtggata gaaaaatcca caaaacccca tgctcaggct
    83881 cgtgaaaata cagattatgg ttatttatgc tggttacaaa aatttggctc taatgacaaa
    83941 acctactttg ggtattatat ggccggtaac ggtggaagta aaatcgccgt gtttcccgag
    84001 ctacatctca cggttgtaat cacaagtacg tggtacggta ctggaaaagc acacgcacaa
    84061 agcgaaaaaa ttttatctga ctatattatt ccagcggtgc aacttgaatc gaaataaatt
    84121 ttatctattc ttaattttat tttttttgga atcttgctcc accggatttc cggaaatacc
    84181 accgggtgcg acacacaaat ttcatgcaac tgattttcct gattcttttg tactgcttac
    84241 agttcctaat aaatacgaat caaaacgccc gaccccagtg cttattgctt tgcatggctc
    84301 aggagatagg gcagaagcct ttcatgattt ttggaagaaa tcagcggatt ctcttggtct
    84361 catcctagta acgccgcaag gattagaaaa aatgaatatg ggttttgggt ggaatcgcaa
    84421 tgaggctacc gagcgttata tcaaaaatgt tttagttgat gtacgaaaac gtattcagat
    84481 agattccaca caaatttatt ttgccggttt ttcatccgga ggatcgctgg cggcttatct
    84541 gaccttaaag aacacaatac ccaatcgagg gacggtttta tttggagcct ctttggatac
    84601 gcattcctcg ttatttaact caggtgaaaa agctcggttt tatattggac atggaacgtt
    84661 agaacaaaat tatccacata ttagttctgt agcggataca ttagcccaat ccggccatag
    84721 tgttcgtttt gttccttacc caaacatagg tcacacgata cctgaaccga aacagaagga
    84781 attactcaat gcgcttaggt ggattttaga aaagtaaaag acacattaga tatttcttaa
    84841 taccgaataa attagccccg cgatataaag cggggcttca tggagactag tggaaatata
    84901 ttgtttcttc tattatttct aaaaggatta tgttttagat agttacacgt attacttaaa
    84961 atcattcaca caacaaaaca acttgcagtc gatttcacca cttttgaatg tacgtacggg
    85021 ggatatagcc ttttttagct aagcggggga gttctttttt gaggacttgg taggttgcag
    85081 atgaatattc acagacgata aaagctcggt tcgccgcttt ttcaaggttt tcatataacg
    85141 cttgggtaag tctttcttga tcaccgcctt tttcatcaaa ataataaata tattttgacc
    85201 acggcatata ggtcatcaca gatttacccg ttcgttctgc ttcatgggct atgattttat
    85261 agttggattc cgttttaggt acaagaatgg caaaactttc aggcaattca acaaatgaac
    85321 gcgtcagatc gtcaaaattc tttccaaaac ctgtcaccac aaaaaacact tctttgccca
    85381 taggccgcaa tctgggattt acagtaaatt caaggcgact gtatatcaga ttacgacgac
    85441 cgatatccat agtaaagtca ctggcgccgg cctgacggtt gagtttttca ctgacactaa
    85501 agatatccca atcccgctgc tcggatagaa cattcatatc gagcacaaaa agcgtatttg
    85561 gaaaattctt tggaagctgt atgactttac ggatatagtt gacattttcc agttgaatgc
    85621 gttcactttt gataaaacca ggttggatat caaattcatg agtcaggagc gaatcaatgg
    85681 cctcatcaaa aggtacatac ctattaagtt gttccaaact gacaccgatc gtaagtcgtc
    85741 gtggttcagg aataacctgt tcagctacag gttcttcttt gatataagca aaaggatctt
    85801 cttgcttaga aacacgtgtg atggaaataa tgaggtgaac cgtaagcaaa accaatataa
    85861 gcgctccaag cgctttcact ataaatttct tattttttaa ccattcattc ataatttagc
    85921 gtgttcgtct ttggcaaact cgaatatcca ctgcaaaagc aggttatatt tttcacccaa
    85981 cgtcactttg cgccttgcca ttacagtatt tgcgacctgc atcgctttat ccattttata
    86041 caatgttatt ttcggcgaat ctacccacgc ataagatggt ataactttgg gggggaaccc
    86101 tccaccgaaa acattgctgc acacacctac taccgtaccg gtgttgaaca tcgtattgat
    86161 cccgctttta gaatgatctc ccatcgtcaa gcctacaaat aaagaaccgg tattaatttc
    86221 tgctccattg atcgtaacgg ataccggacc ataattgttt tttaagtcac tcgtattcgt
    86281 atcggcgcca agattgcacc attctccgat atacgaatgt cccagaaaac catcatgttg
    86341 tttattgctg taaccttgta tgatcgagcc ttcaacttcg cccccgactt tacaaacagg
    86401 gccaatggat gtactgccgt aaatttttgc accgattttt atcacactat ggtctccgat
    86461 atagcacgga ccttcaatga ctgtattagg catcacttga acatgtttgc cgatcataat
    86521 agggccgtgc tcagcatcta gcacaacgcc tggttttata gatgaccctt cgccgatcgt
    86581 gatcgcatca gcatttacta aatgcacacc cgcataaatc gttgcctgtg tatgacccga
    86641 tttgtttcga tcaaaatctt cacgtagaca ctctccatta tatttcacca aatcccatgg
    86701 atattttatc atccgggttt ctgcatccaa ttttacaggt ttcacactat tccaaaagcc
    86761 aactataact ccctgattta taaactctcc tccgctatgc tcagacattt gttgcatcaa
    86821 tgcatcattc ataagcacgc gcccgttgat aaacaaacat tccccggaaa ttttatgatt
    86881 gatccggcga tcaggatatt gctcatgata caccgaagcc agcagcggtc ggcattgata
    86941 aaaaatttct gcctctggga aatatctagc tatgcgctcg gcaagagttg aagcgccgca
    87001 acgtaattcg aataccggcc gtgtcagtgt gagcggatat aaatttacgt agtggtcatc
    87061 ttcgtatata cagatttgca tgtatttatt ataacacatt taatgtgatg atttacgacc
    87121 ggaaacactg aatcctttcg atacgaaaaa gtaactgaac ggcttaaata caatgagcat
    87181 tttgactacg ttttttctaa aaaaagtatc tgttgtaatg aggtctggta aatgtattgc
    87241 attttcaaat cgcgcactcc ttatttcatg agccagtgta taaggattga actgtttctc
    87301 cttaggcatg tcatacttcg aatcataagc cataacacct cgacgataca cagaattagg
    87361 catgattcct gtttttttat acttcaaaat tgcgttatca atttgctgtc ggttaaaatt
    87421 agctgttcgt tcagccaaag attttgcctc tttagataat tcaggaaacc gttcgactat
    87481 atattcatag cgatcttgtt catatgaatt gatatgcatc gttttgccgt cattgctttt
    87541 tagatcaagt ccatattttt cccatgattc ccaaatattc gcaacttctt ttcgacgtct
    87601 ataattaagt gcgttattat catcaatgat tagcaatgag ccgtttggag caagtaattt
    87661 aaaacaagaa ctcaaaaaca acttatgatc gtacatatgt gatatagcat caacggtgag
    87721 tatcaaatca taatatgagt ctagagagta ggtgtgaata tcttgtaata ttggtttaac
    87781 agttggatgc agtccgagaa tggggaacat tttttccatc cccttaacaa acatttccat
    87841 tacttctaag ccaactactt cttttgctcc catagcatac atataaacac actcccaccc
    87901 agccccgcaa ccgaccacta agactttttt gttttctaaa tcaattccgt gatatcttaa
    87961 aatcgtatcc catgccaata gtttacggtt taatttcgat acactactca tcgtatttat
    88021 tttacgatca aaatacttct cgtaagatgg aaatagcggt tgttcggcgt tgattttttt
    88081 tactaattga attcgctcaa aaagatcatc gatagagatt tttgtattca tttgttttaa
    88141 taaggtattt aaaaaattga ttgtttatct gtttacgcaa gaccaagttt attgacatcc
    88201 tgcccgccgt ctctgaaaaa tttaacggga tcgggtacgg agggaaattc acgcacaaga
    88261 aaagattctc cgtactttgt atcaatcaac gaaccaaaat gcgccgcccg gttacgaatc
    88321 gcatcagtga attccggaga actgatatag gtttgcggtt cgtcggattt gtaagtcggg
    88381 ttgaaaaatt gcgacgcaaa tgcagccatt gctttcattt tgatatcgat cgtttctgta
    88441 atgtctacga taaatatatg cccgtctatc cggctaaatt cgtaccgcgc agcataatat
    88501 ataaccttcg caggcctaaa cttctcctgc ggtttccctt tgtccgacgt ctcaattttg
    88561 gaaagaccgc tgtaaaaaga cgattcgcgt actaaatcac tggcatgcat gtgatcggga
    88621 tggcgatcat tccaatattg gcaaaaaaca aattctggtt ggtattttct aatgatatga
    88681 atggctttga gttgattggc gcgatcattg acaaactcgg tatcgggtaa ttctatgttt
    88741 tcacgcacgt gtacgcctag tatttttgct gcatcttcag cttctttatc acgcatttcg
    88801 gctgatccgc gcgtcccggc ctcgcctcgt gtgatgtcac aaatcccgac tttgtatcct
    88861 agtttgacca atttagcgac ggtcccgccg gccgataact ctacatcatc agggtgcgca
    88921 ccgaaaaaca aagcatctaa cttcatgtat gatctcttta atttttgtcc atgttacaaa
    88981 acactatagg aaaattcaag gtgaatgact tacctgaaag gatcaacgtc aaactaattt
    89041 ttcttagact ttgcagaacg gatttgattt tgaaagtatg aacagttttc attgataggg
    89101 cattcttcgc atttggggtt cgtgggtcta caaatttcac gtcctaaaaa agaaatagcc
    89161 atacctacat caccccaata tttcttttca acgaccgcca ttagatcttt ttcgattttt
    89221 tccggttttt cttcttttgt aacacctact ctgggcgcca cacgtacgac atgcaaatcc
    89281 acgataattc cttccgctgg tcctccggat tcgcgaataa tcacactggc tgatttgcga
    89341 cctattcctt tgagtgatgt caaggtttcc atcgtagtcg gtatattttc atcggaaccg
    89401 acagtttttg caatgccggc tagccattta gctttgttac caaaattgcg cacatctcgt
    89461 atgtgtgacg cgatttcatc ttctgtcgcc ttggccagtt cgcgcataga cggataaaca
    89521 ccaaaaaacc tcgacccgac ctcgttaata tgcttatcgg aatcctgcgc cgataaaagc
    89581 gtcatgacta aaagctggta tctgtttttg tattctaaag gatgcttttt gtttttatac
    89641 tttttgatga tcggcatcaa ggcgtcgttc caattcataa acctcctttg tatttatatt
    89701 ttcagaataa gctttcttgt accaccccgc cgcgtagaaa actcgccggc tctccgcgta
    89761 ccactttttt atcgaatccg tattttttat aaaacaggtt aaacaagttt gatatgtggt
    89821 cggccatatt accttcgcca cgcatgcgtt ttccaaattc cgtattattt agttttcctc
    89881 cgcgcaccga ttggatggcg tgtaatatct tttccttttt attgggctca tttcgcgtaa
    89941 gccaatcaga aaaaagctca cgcaactggt acggtaatcg aagtacggta tatccggcac
    90001 tggtcgctcc cgaatcagca gctctcctta atatttcggg tatttcgtga tcgttgagtc
    90061 cggggataat aggagccgtc aaaacaccca caggaatatt gtttttagct aataattcga
    90121 tcgccttaag acggttatcg ggtcgcgatg tgcgaggctc caatctacgc gcaagacctg
    90181 tatccaacgt cgtgatcgag atcatggccc cgaataaatt cagctttgaa agttgtgaaa
    90241 tgatgtccaa atcacgcgtt ataagataat ttttggtaat cattcgcacg gggtttctat
    90301 atttgagaaa cacctccaaa catgcacgcg taatttttaa ttttcgctcg atcggttgat
    90361 aacagtctgt gttactagaa aaagcaattg gttccgcttt ccacgacggc ttcaaaaatt
    90421 ctttctcaag tagttttgcg gcatcgtatt tgacgtgaat tttagtttca aaatccaaac
    90481 ctaaggaatg acccaaaaat tcatgtgaag gccttgcata gcaataaaca catccgtgtt
    90541 cacaaccccg atacggattg attccataag taaaaggtat gtcgggactg tcattttttg
    90601 aaagaatagt tcgtgatttg tcttcgataa gttccgtggg tacagttcgt tcaggatcta
    90661 tatcttcttc tgtaatatcg tctatgcttt tatcctgatg ctgcgtatca aaacgattgg
    90721 gtggattaaa cccggaagaa cgccctttga tgttggttgt aaatggcgtt tgcggcgcaa
    90781 tttcaaaatc attatattcg ctcgcttcat ccgattcgta ttttacgatg cgatatgaat
    90841 ctgatttttc ttcatgtttt tccggctttg gcatagcatc aacttatcgc atttaggagc
    90901 aagacgcaag cctgccggtt gtaattcaca tacaataacc atgatttcat aatttggagt
    90961 tttcgcatgg aataccttcc gtcttctaag aatccgattc attctcagcg ccgtcgagga
    91021 aaattttatg atgtgagtga tatcttggct caaaaacctt cgacgatctc cggagaagat
    91081 atacagtttt ttgaagaact tgatcttgtg taccgcacaa tgtgtgggat tctctataat
    91141 tttgtaccct tatccggcca tcctggtgga agtctgtcca gtggacgtat cgtcgagggt
    91201 cttttatacc aatccatgga ctacgatttt tccaagccgg atatcgatat caacgacatg
    91261 atttgctaca ctgccggcca taaagccatg ggcttgtatg ccatgtgggc gttacgcaat
    91321 gagttaattc gcatagccaa tcccggaatg cttgcccccg aacgccgtca attgcgcctt
    91381 gaagatcttt taggttttcg taaaaacccg acgcaaggta cgccattatt tgaaaaattt
    91441 aaatcggttt cactggatgg ccatccgtca tgcattgttc cttttgtgaa aacggcaact
    91501 ggacctagtg gtgttggcgt acctgcggca ttcggtcttg cttttggtgc actggacatg
    91561 taccccgaaa atcctccccg tacacattta ctcgaaggag aaggcggcat gacaccgggt
    91621 cgtgtttatg aagctatagc cgcagccgct tccgctcagc ttcgtaatgc ggttttacac
    91681 attgattgga atcaatcaac gattgattca gaccgatgca ccgctgaagg tgataaacac
    91741 ggcgactacg tacagtggga tcctgttgag ctcatgtatc ttaatgactg gaatgttatt
    91801 tttgtacccg aaggccacaa ctatcatcag gtactcacgg ctcaaaaaat tgctctatct
    91861 ctccaatccg atatgcctac agctatcgtg tatcgcacgg tcaaaggtta tacctatggt
    91921 gtcactggta aagcttcaca tggtgccggt tataaatttt gttcgcctga gttttataat
    91981 ttcatcaaac cctttgaaga acaatttggt gtgatgtttc ctcgttttgc cggcgatgca
    92041 aaaaaccctg tggatgttga aaccttttat tggaatacgc ttttagtcat tcgtgaagta
    92101 cttgaaaaaa attctttttt accgcaagaa gctgccaaac gccttgcctt atctaaagat
    92161 cgtctatact ctcataaccg tacccaccgc gcacatgcgc caaaactagc caatatttat
    92221 gacggcacag tcaacttaag cgcaatgcct gacgatattc gtttggagcc gggaacatcc
    92281 accaccattc gcggacaagt aggtaaagtt cttaattatc ttaacaaaaa atcaaatggc
    92341 gccttagtag caacggccgc cgatcttttc gaatcaacaa gcattgcggc tgttggaaaa
    92401 ggatttccgg acggtatgtt taacgcaaaa accaatccgg gtgcgcgttt agtacctgtg
    92461 ggtggtattt gtgaagacgc gatgggtgct tttatgtccg gtgtttcaag ttatcgtaat
    92521 catatcggca tcagcgcctc gtacgcggct tttattgtcg cgatggaaca taccgctgcg
    92581 cggtgtcatt cgatcggtca gcaaaactat tgggaacgta ccggcgaacc gatgcataca
    92641 tggattatgg tcaatgggca cgctggtcct aaaaccggag aagacggacc gacacatgcc
    92701 gacccacagt gtttgcaact tctatgcgaa gatttcccct caggcaaact tataacgcta
    92761 acaccctggt caccggacga agtgtggccc cttatgatcg aagggatcat gaagcgaccc
    92821 gccgtgcttg caccttttgt aacccgaccc gccgaggcag tcccagatcg tgtcaaactc
    92881 ggattggatc cagcgatcgc tgcagctaaa ggcgtttatg cgtttcgccg agccaatatg
    92941 aatgcgcgtg aatatcacgg caccttggta ctgcaaggct gcggagtagc cagtatgttt
    93001 atcaatggcg ttttggaaaa aatcgataaa gccggtttga acatgaatat tttttatgtt
    93061 gccagcactg aacttttcaa actccttcct gaaaaggaac aggaggctat ttttccggaa
    93121 catctccgta ctcacgcaat gggtttgaca gattttacat tgccgacaat gtatcagtgg
    93181 ataaggtgga atgaaggact taaccgcaca ctgcatccat tccggcatgg acaccacctt
    93241 tctaccggat cggctgaaaa agtgcttgag gaagcagggc ttcacagcga aggtcaattc
    93301 aaagaaatta tgaagtacgc acgtatgatc gaaaagatga atttgactaa acctcaaaca
    93361 gattcagtta aagctgaaga gaaaattaga tcgaatcgag gtcgtccaaa aggatataaa
    93421 accaaagttg ctcgtgtacg aacgacaaaa ccagcaaagc agcaaataaa aaacacccgt
    93481 gcaaagtcca atgaacacat taaccgaaat aaaaaagcgc aaactaagat tactatcaaa
    93541 ccgggacctg gaagaccaaa aaagaataaa ctcgttcgcc tgaaactaaa atcacaaacg
    93601 cagaagaaaa gtcaaacgac aaagaaaata aagccaggac caggaaggcc aagaaaaaaa
    93661 tgataaaaaa acagcaataa cgaaaagccc cgttaaattt attagcgggg ctttttacta
    93721 aacaaaaaca tattcgaaag agtcgatctt aaatgtataa atcaaaattc tattttaaat
    93781 ggtatcttta ccaattccca atgcaaataa accgtacatg atttatcggt tacactctca
    93841 aaaccatacg ttagaacttc ttgaaatttt gcagtttctt ttttaaccgt tacgcgtaaa
    93901 gcatcttcat tttgatcata ggaataactt ccccaagctg ttgagctttt gttaaaaata
    93961 acaacgaact ccgattctcc tggtatcata tgcaaaccat acgttccggc tttcaaagtt
    94021 tggccttcga ttttaacgtc tttgcttaca gtgaaagtcg tattctcatt cgctccggca
    94081 cgccaaggta tcatcttgcc gtcacgattt tttgtcaaac caaacggaac aagctctccc
    94141 catattttcc tgcttttcac agaaggttgg ctataattaa ttgatatttt ttctgaacct
    94201 actttttgct ctgatttggc cggaggactc ggttttttat tatcttgaga aaatacaaaa
    94261 cccgatagca ttagactaaa cgcaagataa cgaagtgttt ttttcatgcg gatctccttt
    94321 tatggtgtaa ttaaaaacgg ttaccgaaaa gtaaccgttt atacgatgta aacttttaaa
    94381 agtttgtttc tcaaagcgat tttaaatttc aatattccac gtttttaatt catccaaaaa
    94441 cttatgtgcc gtaaggtcta tttggattgg ggtcacggaa actttgtttt ttaggacaac
    94501 tacatcgtca acatcatctt catgatccaa tatcattttt tttccggtca accaatagta
    94561 cgcacgaccg cttaaatctg ttctttgctc aaactcttct tcatatcgcc cgcgaccctg
    94621 ccgtgtcact gcgattcctt ttatggacgt acgaggaccg ggaggaatat ttatatttag
    94681 tagtgtatcc gaaggcaacc ctttatccaa aacaatacgc gctatttttg ccgtaatttc
    94741 tgcggcaaat gtaagatccg ttttatgatc gaaagcaatc atagatgaag caatagaagg
    94801 tatgcccatg ataatacctt ccgtagcggc ggagacagta ccggaatata gcacattagt
    94861 tgcggtattc gatccgtgat taatacctga aactatcaaa tctggttttt tatgaaccaa
    94921 gttttttaca ccgattttca cacagtcggc aggagttccg gaaattgcgt atcctttgaa
    94981 tttttctggc cgatgatagt caagcacacg aataggatcg tgtatcgtga tggcatggcc
    95041 gacagcgcta cgttctatct gaggtgcgac gacagttaca tcccctaatt tttttaattc
    95101 tttatataac gcaaaaatac cattagcatc tattccgtca tcattagtca gaagtatgtg
    95161 tttcatttta ttcaaaatta ttatttgtat cagcttttgg ttggtttgaa ttgggtgtcc
    95221 gtattcggct caaaaaattt aaacaacctc gagagcgttt ttttaagttc atttcttggt
    95281 actatcaaat ctacaaatcc gtgctcaact aaaaactcgg ctgtttgaaa tccatccgga
    95341 agatcttttc ctgttgcttc tttgaccaca cgaggaccgg caaaaccaat caaagcccca
    95401 ggttcagcta tgattacatc acccagcatt gcaaaactag ccgtgactcc gccggtcgta
    95461 ggatctgtaa gcaatgaaat atatggaata cctttttcat ggagtaaggc aagcttcgct
    95521 gatgttttcg ccatttgcat aagcgaaaga gctccttcca tcatacgtgc tccgccggaa
    95581 gctgaaacga taatcatcgg gagcttgttt ttgtatgctt gatcaatagc tctcgatatc
    95641 ttctcaccta caacagatcc catgctcccg ccgataaaac caaaatccat gattgccacc
    95701 attacaggac gaccttcgat ggtaccataa cccgttctta tggcgtcttt tatcttcgtt
    95761 tttttaatca tcgcctggat gcgatcgctg tactttttgg tatcgacgaa ctgaaatgga
    95821 tcaactgaaa tcatattaga ttctgtttcg acaaaggagt tttcgtctaa cataatttca
    95881 atatacgtgc gtgcaaatac gcgaaaatga taaccacatt ttacgcacat aaaataattt
    95941 ttttctacgt cttttttata ttgaacttcg ccgcacttat cgcattttct ccacacattc
    96001 ggcaactcac gcttttctgt gctttcatta acaccgtctt tttttctttg aaaccagttc
    96061 atagtctcct caatatttct aaataagatt aagatccatc aataaggcgt cttctgtatt
    96121 cataaaacca tcatgataat agttttttct aacacctact ttttcaaata gaaatttttg
    96181 atacaatgcg atcgccgtcg tattggactt tctgacctct aacataacct tcacacattt
    96241 gttttgacgc gcgatagaga tgatgttttg catcataagt ttacccaacc cgcgtcctct
    96301 ataatccggt gtaacagcga tattagccag atggcactca tccaatatta tccaaagaac
    96361 aatgaaaccg attatcaatt tgttttgttc caaaaccaac ggcaacgcaa tatcggtatt
    96421 ttttaattca tctgcaaaat tacttcgcgt ccacgggctg tcaaatgatg cgccttcaat
    96481 tcgcatgacc gtatcaaggt cttcgtttgt catcttgcgc aatattaaat ttccgggaat
    96541 tatcaactca ttaaagttca tgaagctccg cgaaatcctt gaaaataatc cggaaccaca
    96601 ttatccaaat cagcatatat acccgctata gctttagtta agccgatttt accgacggaa
    96661 aacacattgg gtaaaccgtc ttgagaatta gaaatatgaa tacctgataa ttcactaaat
    96721 gatattggat catcagttag aagtaaatct ccttggagca aattattttc gagttcatca
    96781 aacttccata cttgtccctt tatcaaaaca ttatggtctt caaaaaatgc gatataatat
    96841 tctttttttc tcgaagtcag agcaacccat atacgccgat aatgatctat ccacggcagc
    96901 gcgatggctt caagtgtagg tacggttacc aacggtactt taaaagaaaa agctaatcct
    96961 tttgctacag ataaaccgat ccttaagcct gtaaatgatc ccggacctat tgtgacggca
    97021 atggcttgaa tatcttttga actaatcgaa ctttgttgca ttatgtattc tacaagtaac
    97081 atcattcgct ctgaatgtgc gttattgata ttgagatgaa tacttgagat aagcttaatc
    97141 tctgattcgt taccttctac tagcgcaacg ccgcatattg atgttgatgc atctaatact
    97201 aataatttca taattcgcgt cgaatactaa tttgcctttc ttgttcattt ctcttttcaa
    97261 aatagactcg ccacgatgat tgaggtagtt ttttttcaac acgttcagac cattcagcaa
    97321 ttactacata atcttcttga tccatcattt cctctatgcc caattcggca atatcttttt
    97381 ctgtatttat gcgataaaaa tcacagtgtc ttatgataag agcttttccg tttttatacc
    97441 cctcgtattc gtttactatt acaaatgtcg gactatgcac gggctcctga acacctaaac
    97501 ctttacatag acctttcgca aatatcgttt tcccgctgcc aaggtaaccg aaaagtgcta
    97561 taatatcgcc gggttttatt ttttcggcaa aatcttcagc ataatcaaaa gtttcttttt
    97621 ctgaatgcga tatataatcg ggtatcattg caattcggat gcggcttctt tatcaagata
    97681 ccaatagagt tcaccggcgg gtcttaccat ttgagccggc agtatatctt ctgtactccc
    97741 ttgaagtact tgtttaataa tagatgattt tgatttaccc gaaacaagaa acaaaacatt
    97801 cgaagcatga ttgatggtag gaaatgtaaa agttacccta tgtcgcccta atttttttac
    97861 aaaattatcc attacaagaa actttgtttc ggtaattcca tccgttcctg gaaaaagcga
    97921 cgcggtatga ccgtcttctc caaggccaag aagtatcaga tcaaattttg gaaaacctcc
    97981 atccgacaac ccaaaaaaat ctttaatgtc atcttcagct tgctgtgcgg ctaacatgca
    98041 gtcttcttct gtaccaatac gatgaatatt ggcatcagga aaccctattg ggtccatcaa
    98101 tacatttttt gccattccaa agttgctatc tggatgtgta gaaataacat cgcgctcgtc
    98161 accccaaaag aggtgtactt ttttccaatc tatagattcc ttgtgttttt ttgccaaaac
    98221 ttcataaacc attttgggtg ttttgccacc agctaaagac catgaaaaat gtccatgcat
    98281 ggatatggct tgatgaccta tggatattat tttttcagcc gttttttctg ccaattcatc
    98341 cgcagaagta aaaaccgtag tgtgtgtttt catagttttg atcggttagt gttttaggca
    98401 gacgtattta ggcaagaatt gtgttgagta tcgtttggaa cattttaaat ttgaaaaaaa
    98461 gaatatacaa aacaaaggac aattttgtga aagaagtttt tattcttagt gctgtccgaa
    98521 ctcctattgg aaaatacggc ggtgtgtttt cttctatcaa agcaaaagaa cttggaataa
    98581 tcgccgcagc agaatcaata atgaaagcag gattaactcc agactctgta gatgaagcta
    98641 tatttggatg cgcgcgacag gccggtatcg gacccaacat ctcaagacaa attgctgtcg
    98701 gtgttggtat tccatcacaa aagactgcat atacgattaa taaagcttgt ggctcagggc
    98761 tcaaatccat tatcaatgcc tacacctcca tagttctcgg tgacaacgat gtcgtacttg
    98821 ccggaggtgt agagagcatg agtaatattc catatttact tacccaagca cgttggggat
    98881 atcgtatggg taatagtgaa attttggatg ctaatcatac ggatggatat ttttgtccga
    98941 tggctgataa actcatgggt gctacagtcg aagacctggc tctaaaatat aatattactc
    99001 gcgatgaaca ggatgctttt gcattacaat cccaattgaa agcagagagt gctatcaaaa
    99061 ccggtaaatt taataatgaa atcattcctg taaaagtggc taccaaaaaa ggtgatcaga
    99121 tcataacgac agatgagcat ccacggattg gcgccactat tgaagatttc aaaaaattaa
    99181 aaccggtttt taaaaaagat ggtacgatca ccgccggctc ttcgtccgga gttacggatg
    99241 gcgcatcagc cttgataata gctggaaaag aatttgtaca aaataataat ctgaaacccc
    99301 ttgcccgtat tatcggttat gcatcagcag gtgtcaatcc ttctgaaatg ggtattgcgc
    99361 cggtacccgc tgtacaaaaa cttctaaaaa aatcaaactt acggcttcaa gatatagatc
    99421 ttattgagct caacgaggcg tttgctgccc aagcacttgc ttgtgaccgt gaattacatt
    99481 ttgataaaga taaagttaat gtacatggag gggccattgc tttgggacac ccgacgggtt
    99541 gtacgggagc tcgtattaca acaacactag ttcattcgct tcatgatcgt aaagcaaaat
    99601 tgggaattgc gaccttatgt attagcggag gcttagggtt agcgttattg attgaaaagg
    99661 tataaaaaag aggggcttga gtgaaggatc ccaagcccct gatcgtctat gtgaaggtga
    99721 aaggaggagt tttattaaaa cgccgccagt tcaatttttc tttttatgat ctcaacgtct
    99781 tcctttattt ttgtgtttga tttcatccat tcttctacca ttttgcaagc gtatatcacg
    99841 gtgctgtgat cccttcctcc aaaatggatt ccaatagttt ttaaagagct tcccaagatt
    99901 tcttttacca aatacatgca tatttggcga acttcggcta tttccttttt tcttcccttc
    99961 gtaagcattt gatctacggg aatcgaataa taatccgata cgatcttctg aatggtttcg
   100021 atggataaat tttttctttt gctggctatg gtatctttaa gcacagcctt agcaacatca
   100081 agattgagct ccgtattggt tatcgaggca taagcaagca atctgatcaa agaaccttcg
   100141 agctcgcgaa tatttgacgt aatattcatt gcgacaaatt cgcatacatc cggaggtaaa
   100201 tatacaccgt cttcttcgct gcgtttttgg agaatagcaa tacgggtttc aagatccggc
   100261 ggctgaatat ccgttaccag tcccatttga aatcgagaaa tcagacgctc ttcaacttgt
   100321 ttcaactctt tgggcggacg atccgatgtt agaataatct gtttacccag ttggtgaagc
   100381 gtattaaatg tatgaaaaaa ttcttcctgg attttccctt tatcggcgaa gaattgtata
   100441 tcatccacca tgagcagatc gaagctacgg tataaacggc taaactctaa aacatcattt
   100501 tttcgaatcg cagcaacaaa atcattcgta aaagtctcac tcgtcacata acatattttt
   100561 ttttcaggga agtttctttt tacctgatta cctattgctt gaataaggtg cgtttttccc
   100621 aaaccggcac caccataaat aacgagtgga ttaaatgacg ttttacccgg tttttcagaa
   100681 acagctaaag cggctgcttt tgcaaatttg tttccatttc cttcgatata gttatcaaaa
   100741 gtatatcgta tgttgataaa gctgtcataa actactgggg ctgtttcttt aggtttgttt
   100801 acagaagctt gaagaacgag cggggtggat ggcgtagaaa ctgtttggat cggttctgta
   100861 atttctttta cgatgtaggt taccccgatt gaacgaccac aaacttcgcg taatgtattc
   100921 aaaatcaaat cgttataacg actttctaac cattctacaa aaaattgatt aggtataaat
   100981 gcaacaaact tctcgttttc tataacatga ggctctattg ggttaatcca tgttctaaaa
   101041 gatgggccgg gtatttgtga tttgattata tcacaacagc gattccatac cgatttggca
   101101 tcaagttcga ttatcgctga catattaccc gattctgttg tagtaggctc aaccatgact
   101161 taaaaacaag ttattaacaa tacaaacacg tgtacaaaaa aatcaacaca ggttaaaaaa
   101221 acaaataaag atcaaatcaa aaaaaatcaa atcaattatt tacaactgtt tgaataaaaa
   101281 aattaagaaa aacgagggga taattagtaa ataaatgaaa acaacaacac taataatgaa
   101341 tatataaaca aattatcaac acaaaacaaa cacaatggaa cacgattaca atttatcaaa
   101401 gacaaggtct ttgtcaagtg ctttgtcaaa gcatagtata tgtggatagt tatctttttg
   101461 ttattttggt gtttatttct aaattttttt tatacttttt tttcttgaca agtatgctgt
   101521 caacagcaga attttataaa actaatagat tcaaatatta gcgtggatta aatatgaaaa
   101581 ctgtttattt attaaaaaca acaattttaa gcttgacttt ccatacctta tgttctatat
   101641 tccccaccca tttgtaagta tacgtgtatt agattgttac tgattgctac ttatagagtg
   101701 acatacctat ataacacagt aaatggaaaa cgaaaattat tgtttaacta aaaataatct
   101761 gcgaggatca cgtgaaacgt acatatcaac ccagcaagcg taaacgtaaa aacaaacacg
   101821 gatttcgctc gcgcatgaaa acaaaaggcg gaaaaaatgt tttggcatcc cgccgagcca
   101881 aaggtcgtaa aaaactttca gtaagcgacg aataattttt tttgaaatta agatttaata
   101941 aggcttcttt caaaatacga gagaagcctt atttattcta tggtatgaca atgctccgac
   102001 gttctgtact ttattttttt gtcagcctga tccgtattta ccaacatacg ctttctgttt
   102061 attttccgtc ctcttgtcgt ttttatcctt catgttctca atatagcatc gaatctttaa
   102121 aatctcacgg tttgttcaaa ggcctttggc tgacgatccg acgcatatct aaatgccacc
   102181 catttcatga aggtggatat gatcccgtgc cacaaaatca aaatactaat aataaatgct
   102241 gctgatattt tctggagaca aactcaatgg atagacgtac gattttcggg ctttttatga
   102301 ttgcccttat cataatcttg tatccctttt atatgcaggc tgtcgcgcca cctgcagatc
   102361 cctctgttaa aaccacatta gctgttgata gcacctcaac tccaactcat cctgatactc
   102421 ttttgcagtc caaaaccaac atttcaaaag aaagcgatct acctaaaacg gttagcttga
   102481 caaaagatac aataaaacaa tctgaaaaaa taattcgtat taattcagaa atatattcag
   102541 ctgaaatatc aaatctcggt ggcggatcca ttaagagctg gaaaatactt ccttacaaaa
   102601 ctcatgatgg acaacctgta gattttattg aatcatcaaa aaatctaaac ctgaattttg
   102661 tttttcaagg gaaaaactat aatacgtgga atataccata ctccattatt aacacggatt
   102721 cagttattga tttaataacc aaacaaaaaa acactttaac gtttgtttat cattttgatt
   102781 ccgaaagaca gatcattcaa aatctaactt tttataatga tagatacgat gttgatatag
   102841 aagttcagtt taaaaatttt ggcgacgata taactaatgg tgaataccaa atacaatggt
   102901 tgtcagcact caagagcact gaacttgatc gcaacggaga tctaagtcat accatatctt
   102961 atgcttatat gggcgatgaa aaagaagaat ttgatattgg aactgatccc tccaaagaaa
   103021 ccacctggtc cggagttacg aaatgggttg cttctcgaac aaaatacttt gctgtatata
   103081 ttattccaca agaaaaagat gggattggtt gtaaactaac aagcacaaca actcacgaaa
   103141 atggtttact tacaaaaaaa tcaaacttca tgaatattca aatgaagttt gaaccgaatg
   103201 ttgttgatcg ttacaaattt tatattggcc cgatggatta tcatatttta aaagaatatg
   103261 atgataatgt agctgtaacc atggaatggg gttgggccgt atttaggcca cttacgaaag
   103321 ctatttttta tactttcaac tttctttatg acataatccc aaattatgga tgggttatca
   103381 tagtttttac tcttttagtc agaatagttc tttatccttt gacttataag actttcgtag
   103441 gcatgcaaaa aatgaaagtt gttatgccgc ggcaaaaaga gattcaaaag aagtacaagg
   103501 ataatccttc tagaatgcaa caagaaatga tgaaacttta caaagaagcc ggctacaatc
   103561 ctctttcagg ttgcctccct atgttaatac aaatgccttt attgtttcca atatatcaag
   103621 tttttaacca aagcatagag ttgcgcttgg ctcctttttt tggatggata accgatttat
   103681 caaggccgga tacgataact atattacata caggcttacc ttttatcggc gatttccacg
   103741 taaaccccct tcctatcatt atgacggtgc ttacattttt acaacaaaaa attgcaccga
   103801 tgacaccgat gggggacgac tctgatccgg ctcaacgttt taatcaaaaa ttccttatgt
   103861 atggtatgcc gattatgttc tttttccttt ttaatggatt cgcatctgga ttggttttat
   103921 actggacagt attcaacgta ttgacgatgg cgcaacaagt aatgatgcaa aaatttgttg
   103981 aagtaaaata acataatcta attttaaagt cactcggata actataggtt tgaagagtga
   104041 ctttttattt taattatttc caaaaatgaa ttttgacgaa acgattgcgg ctataagtac
   104101 ccctatcgga acaggagggg tttgtatggt acgcgtttgc ggtaaagaag ccgttaattt
   104161 agcagatcaa atttttttag gtcattcaaa accaagccaa catataagcc attcacttca
   104221 taacggttat attattgacc ccttaacaaa agaaaagatt gatcatgtct tgatcggggt
   104281 tatgcttggt aagaaaactt atacaggtct tgatacgata gaaatatatg gtcacggtgg
   104341 ggtattaaac gctaaaagga ttttagatgt ctgtatcaag tatggtgcac gtcatgctga
   104401 gccaggagag tttactaaaa ttgcctttct taatggaaaa atggatttat cacaagtcga
   104461 ggcggtggcc gatcttattc acgccaaaac agaaagcgcg agacgttcgt ccatggcaca
   104521 attcatggga aacttatctc atgagatcga cgaattaaaa aaacaactta tcaagctttg
   104581 ttcgcttctc gaattagatc tcgattttgg agaagaaaat ctaatcaata ttaccaaaga
   104641 atccattgat gcgcaaataa ccaatgtacg tctacaaata gaaaaactct tatctacata
   104701 caacacgggc caccttataa gagaaggtgc aaaaatatca atcataggaa aaccgaatgt
   104761 cgggaagtca agccttttga acgcaatgct aaagcgaaat agggctattg taagcgacat
   104821 accgggaacc acgagagatt ttattgaaga aagtttagat atagagggaa taccttttat
   104881 atttgttgat acagctggta ttcgtcaatc taaagatgta gtagaaaatc aaggcatcca
   104941 atcatcaatg gagatagcaa aaaacagcga cttgatatta gttattttcg atggatcaga
   105001 agatttaaac gaagaagata ttaatatttt aaaaatggtt gaagatctaa tatggaaaca
   105061 aaagcatgta aaagccttat tcattgccaa taaatgcgac aaagaaaact ataatccaca
   105121 aagaaccaaa aacgatttta tagagatttc agcaaaaaac gatataggga ttgataaact
   105181 aaaaagaagt ataggcgact tatttcttaa caatttctct attgaatcac ctatgataac
   105241 tcggttgagg cacaaagatg ctttagaaaa cagcgtagta tcattaaata acgcaaaagt
   105301 tagcctacat aacaagatgt cttatgagtt catttctcta gacattaaaa acgctattcg
   105361 tcaccttgga gagataattg gagaagttac tggagacgat gttttaaaca acattttttc
   105421 aaatttttgt attggaaagt gaattaactc gtgctcctaa aaacagaaaa ttacttttta
   105481 aataaaacat gtttataata aataggagca tgtttcacgt gaaacaaaaa gtaaaaaact
   105541 aatatttcat aaattttaac tatcaaactg tttcacgtga aacaataaaa agcagatgga
   105601 tgtttttgat gtagtaatag taggtggggg gcatgcaggc tatgaagctg cttttgcagc
   105661 atctagactt ggatgcagta cacttcttct tagtatggaa accactaaaa taggaacatt
   105721 atcttgtaac ccggcaatcg gtggcacagc aaaaggtcat ttagtaaaag agattgattc
   105781 gcttggcggt gaaatggggt tcataactga tcaggtagga attcagttca gaatgcttaa
   105841 taagtctcgc ggccctgctg tgtggtcacc tagagctcaa gttgatcgag ctgcatatcc
   105901 attaaaaatc caagagcttc ttggtttgaa aaaaaatata tccattcgcg atgcaaatat
   105961 tgtcggtgtt gtagttgagt caaatcaaat taaaggggta attactgata aaaatgaggt
   106021 tattaaaggt aaaactgtaa tacttacctg tggtaccttc ttaaacggta taattcacat
   106081 tggactaaaa aatgttagag caggaagaat aggagaaaaa ccatcgcttg gtataactga
   106141 gtctttaatt agtttaggat ttgaaagttc tcgcttaaaa acaggaactc ctccaagaat
   106201 tcacaaagat agtattaact tttcggtttg cgaaccacaa tatggtgata atccaccaga
   106261 accgttttca tatcgaacag atagtataac caatactcag attccttgtc atcttactta
   106321 tactaacgac aaaactcatg atattttgaa atcaggtttt gatcaatcgc caatgttcac
   106381 aggtagaata aaagggttgg gcccaaggta ttgcccatca atagaagata aaataaatcg
   106441 cttttcagat aaagaaagac atcaaatatt tttagaaccg gaaggttatg attctataga
   106501 atactatgta aatggttttt ctacaagtct tcctgaagaa attcaaatca gcgcaataag
   106561 atctgtaact ggattagaaa acgcacatgt tttaaggccg ggttatgccg ttgaatacga
   106621 tttctttccc ccgtatcaaa ttaatttgaa tatggagaca aaatatgtag aagggctctt
   106681 ttttgcagga caaatcaacg gcacatcagg atatgaggag gcagccgctc aagggatgat
   106741 ggccggaata aatgcggctt taaaagtaaa gcaaaaagat ccatttattt tacgtcgaaa
   106801 cgaagcatat atcggagtac ttattgatga tcttgttaac aaaagcaccc ttgaacctta
   106861 ccgcattttt acttccctag cagagtatcg tttactccta aggcaggata atgctgatat
   106921 gcgccttatg gaaaaaggaa atgcctttgg ccttatcgat gaaaagcaaa tggaaaagtt
   106981 tatcagaaaa aaggatgcaa taaatcaaat agaaacttgg atagaaaaaa ctaatttaca
   107041 cccaaaaaaa atcaactctt tcttaataaa taaggggtcc acaatcttag aagaaccgga
   107101 acccctggct aaaatactaa aaagaccgga agtaacttta actgatttat tagagtgtga
   107161 agaagaaatt ttcgaaccaa aaatagttaa tttattaaaa tatcgagaca ctttaaggga
   107221 agtagatact tcaataaagt atgatggtta tttaaaaaga caacaggagc aggttgatag
   107281 atttttgcga atggagaaca aaaaaatacc actcaatttt gattatgata aaataacatc
   107341 actttcgaaa gaagcgcgtg agaaattaaa aaaagttaaa ccaggcaacg tagggcaagc
   107401 gagtagaatt tccggtgttt ctccaagtga tatatcgcta ttgttagttt ttttggaaag
   107461 aggttcttta ggaaagaata tacaagattc atttaatgac acactaaatt aatatatgca
   107521 aaacaatcaa ataaattggg atgttttttt tcaaatactt gaaaataata cacttaaatt
   107581 aagcagtatt cagaaaaatc aatttttttc ttttcatgat tttcttctag attggaataa
   107641 aaaaattaat ctcatcggcc gtcaagaggg gaatattatt tctcgtcatt ttctgaattc
   107701 tgcattaatc gctttttttg ttaagtttaa aaaagaacaa aaagtgatag atatcggcag
   107761 tggtggaggt tttcctgcaa ttattttaaa tattattttt cctgaaacac atttcacact
   107821 agttgaatca attaaaaaaa aaagtgattt tcttaaagaa acctcaaagg tcgtcggtct
   107881 gaaaaattta gtggtaataa atgaacgagc tgaaaaaatt gcgaaaacaa aatctttttc
   107941 atctcattat gaattcgtaa ccgcacgtgc agtttcagcc ttagatgaac ttattccttt
   108001 gacgagtccg tttttaaaaa acgagggaag gatgcttttt ttaaaaggga agcaggtaga
   108061 tatagaagtt gaatcggtta aaaaatcaaa ttggccagat aaatacacac tgacgattca
   108121 tcgctttcaa aacatccctt ttattcctag tgatcaagaa agcgctttag ttgacataaa
   108181 attttttctt gataggtagt tcgttttttt ttaagttatt aaattataat ccaaccgagg
   108241 gagatcatcc gagaggcctt ttttactacg aattgatttc agcttaagac gaacttatcc
   108301 gaaataaggc tttgattgtg cgattgttga ttcatcaata tgatctgcag gtgggtggat
   108361 tgtttctcca tattatctca aaaagatttc tcgtagtttc atttctactt ttttttctca
   108421 atccgtctaa atctttttct caggatttag atagtatttc cgtttcactc caaaccgctg
   108481 attcaatttt atttttcatt aacgaaaaag atattgaaaa aaacattaat aaaaccccta
   108541 cgtctaagga tgttaggcaa gattctgttt ggagtgatct caatgcaata aaagctttgg
   108601 agacagaatt aataagctta atgtcagaaa ttaacgccaa tctttcaata accggtcaac
   108661 ttgatctagt gataaatcaa gtaattgaaa atgaggcaaa aaaaattaaa gactctaggt
   108721 caacacgatc gttattttct ttatacccat ttcaatatca taacacgatt tctatagacc
   108781 ccaatcaaaa ctacttttta atacgtggtg gttctataga tttttctcaa acacaaaagt
   108841 tgttaccagg atataaggta atagcaatgt tggattcatc gggcaattat gtttttgctg
   108901 acgaatttgg ccaaacaaca aaaaaacaac gatataaaga aacaacagta ataaaacagg
   108961 aatcttatat atcaaattcg tttgtttatc agattagaag tgtttttact aaaactgtgg
   109021 ttactggtat tgcttcaaaa gaagaacgtc aatccggggc cgtaagactc tttagtgcca
   109081 gcgttacaac caacgaaacc tttcaaaaaa tatttggtgg tgatgaagtc accgtagatg
   109141 ctacaggaaa tataaatttg aatgtttcag gagccagcga aaaatcttct aaccaaagct
   109201 catctaccgg aaaaagttct caattcatac caaaattcga acaacaacaa aaattcaact
   109261 tacgtggtac aataggaaaa aaagttgaga tcttatttga tcaagatagt gagcgtgaat
   109321 ttagttttga aaataatata aaaataactt atacgggttt tgatgatgaa attttacaaa
   109381 aattagaggc cggcaatata gatttaagtt tacccggaac acaatacgtg acacagggtg
   109441 gtcagaacaa aggtcttttt ggagtaaaat ctatctttaa atttggagat cttacactta
   109501 catctatcgc aagtatacaa aacggcgaaa agaaagtgat tgaaatcaat cccggttcgg
   109561 gcaataattc gacctcaata ataaaaaaat cagctaaaga ttatgaaaaa ggaaagtttt
   109621 tctttttaga caaacggtat ttaaatcgtt acgaaatgta tgatccccaa gacgggcgaa
   109681 agcacactta cgatcctgcg gatgaaatta tcaatataag gcttttccgc aaaagtgagg
   109741 gcaacgatag aaaccctgaa aaaaggatcg gtattgtaac ctataataat tcaactttga
   109801 caaatccaaa tctgcctaac atgacagttt cacaaataac cgatttggat tctgcaaatg
   109861 cggccactat gattttttta gaaattccaa aagaacagtt ttcttgtaat ttccagcttg
   109921 gaataataaa cctcagtaca aaactcactg ttggtgaggc attagctgtt tattacgaaa
   109981 catctcaaca ggtatttgga gatgtttcat cagacacgtt gaaattaaaa ctcttatgga
   110041 agcccacccc tcttcctaca gattcaactt gggatttaga gttaaaaaat atttacgctt
   110101 ttgacggtat aaccggcttg agtgccgaag aaggagaaag tgtaaaaata aaatttaaac
   110161 catcatccaa taatgatata actagtttcc caaatattgg tggcaaagac cggggattac
   110221 ttgatattct tcacgtggat gaaactagcc aaaacggtaa atcaaaagat ccagatcaaa
   110281 taattgatat cgctccttca tcgacagttt tgatgggata tggtttagat tatgataatg
   110341 gttggattat ttttccacgc ttgcggcctt ttgacccacc cactacaccg cttccgggtt
   110401 ttcgtgacga ggtttttccc gcattaaata atgatcccaa tctcggcgaa gtacgaatac
   110461 ctataatata taatgaagct cgatcatcag acaaatttaa acaatttaac gatcgctatt
   110521 cgatagaaat agaaagcaaa aaaagtagct ctgcaaaatt ctctttaggt attaatgttt
   110581 tagaaagcag tgaagttgtt cgattagatg gcgatgtatt agaaaaaaac attggctata
   110641 ccattgacta tacctcagga gagttgatat taattgatcc gcgtgcagca ttatcaacag
   110701 ccaatattaa gatagagtat gagcaggctc agttatttca agttgataaa aaatccattt
   110761 ttggtacccg cgcagaatat aatttggcaa attacggatt cggctcaaat tcattcattg
   110821 ggacaacggc gctttttcat agtcaatcta cagtaaacaa acgcgttcaa atcaatgaag
   110881 agccttttaa taactttgta tgggatatta ataccaatat tgaattagaa gctaattacc
   110941 taacaaaatt cattaactat attccttttg tttcatccaa tcagaactca aaaatcaatt
   111001 ttgcagctga atacgcaaaa ctgcttccta ctcctaatac aagtaacggg ttgatggaaa
   111061 atgatgaaaa tggtgttgcg tatatagatg attttgaagc aataaaaaga acctatccca
   111121 tgggttcgaa tagaaaatcc tggactcttg ccagcaaacc tattgagaaa aacgcttcca
   111181 agcgtcgaaa attaatctgg tatcaaaatg aaatttcaag aagcaaaata tccaatatta
   111241 aaactaacaa tacagataga gcgaccgtat taactctaac cttacaaaat aaaataagtg
   111301 atgaggcccc aacggaaggc ctatggggtg gagtgatgcg tggtttttcg ttgtcatcag
   111361 ctggagaact tggagcaagc cgttttatcg aattttgggt aaaaaataac actccagagg
   111421 gtaaacacgc taaaataaat atcgagattg gtgaaatttc tgaggatcaa aatggaaacg
   111481 ggggagctcc taatcgagag gtcaaatcag ctttgcagcc cgtaattgaa aaaaatcaag
   111541 atatagggct tgatgatcta agtgatgccg gcgaagatag ccttttatcc aaattaggta
   111601 ttactaggtc tcaatatgga aacctgagtt tttcgcaaaa agcaatagtt gatagtttac
   111661 aaattatttt tccatgggga acaatcgctt tggatcctga tgatccgttt ggtgataatt
   111721 ggaataatga acaagcccgt tttggaaaca aagatatcca aaacgccgaa gctaatcggg
   111781 acataagaaa tatcaaagta aatggctacg aaaataatac ttttcctatt acagatggtg
   111841 gtttaagaat aggggatact gaagatatca ataacaacgg tgttattgac atacaaaacg
   111901 tttatgctcg gtacagtgtt acaacagata cacatagctt agataatgat attatagttg
   111961 gacgcggcga agacggttgg attctctatc ggttacctat tttaaggcca gacacgatta
   112021 tcaattcaaa ttcaatcgac cttgtacttc caaaaataac ttcagcaaga atttggattt
   112081 cctctgaaga tccagagtct gaaataatta gcgtacagtt atctgaaatt caatttatca
   112141 atagtgagtg gaaattgccc atagaggtac ccaatggggt attactaaat cccaatggaa
   112201 aaattccatc aaataacgaa aacagtaaaa tagtagaaat ttcctctatt agtactgatg
   112261 aaggcggtgg ttatacaaga ccaaacggag taaaaagaga aaaaccaatt gatcaaaatg
   112321 ggaataacgc tcaaagagaa gtaaaagaac aatcattact ccttcgtctg aacgaattac
   112381 cagttaactc aaaagcaata attagcaaat ccaactcgca aagagatttt cgaaactacg
   112441 ggcgaattag aatgtttgtt cacggggata aaggcggttc gatacctttg ccgatttcag
   112501 atgaaagtag tgatagtcct ctaaaatatt ttatccgctt tgctgatcga ttcaatagtt
   112561 attacgaaat tgaacaacct ctgtatgccg gatggtcaga agaaaaaaac tcacttgata
   112621 ttgatataag agaaaacctt acttccttaa aatttcagag aaccggcaac gatactctta
   112681 cagaatacaa tatcggcgat aacaagatta ttcgaatcaa aggtaatcca tctttggaca
   112741 atgttcagtt catttattta ggtgtgcaca acacatcttt agtaaacgct tatactggtg
   112801 aaatatggtt taacgagctt cgcgtaacgg atgccgatga taaatccgga gaggccgtca
   112861 tggctcgctt atcactaaat gtgggagata tgctgactct cagtggaagt acggaatatc
   112921 gttctgctga ttttcgaact gtcgaacaac gttttaatcc atccggagga aaccaaaaat
   112981 catggagtat cagtgggaac ctaaaccttc ataaatttca tattgaaaga tggggtattt
   113041 ctcttccatt gagctttaat gcaagtcaca acacatcaga tcctaagttt ttaccgaata
   113101 acgatatctt ggttaccgat gccaaagaag ccaataacga aaaaataaga tcattacaat
   113161 tacgaaacga agaaataaga gatagtctta aagcatggag attgtcggcc tcgatggatt
   113221 ctagtatata tcgtgacagc ttacaaaacg atagcttatt gaaagtagca ttaaatttta
   113281 aaaacatgat aaagtcggaa tctttttcac gaggtttttc tttaaatttt tctaaagcta
   113341 aaaacgaaaa tgacttttgg ctcttgcgat ggacaatcaa caatataacc tctaacttca
   113401 actggagtga aaccgaaacc aatactccaa catcaccaca gagcacttct agagactggt
   113461 caagtaatac ggcttacaat atttctttcg caagaaaatc atataaacct ctccgttggc
   113521 ttccattaac taaaacgcct tttattggtg ttgtttttaa aaatataagc gagaccgatt
   113581 taaattataa attcattact tctgcaaaca ccgatttccg aattgactca aaatatcgtt
   113641 cagaaacaat aagagacgca tacaataacc caataaaaaa acctgttaat accacattaa
   113701 gcggctcgcg aggatatgga ctgagtgtaa gcccttggca gtcattgacg tcctcaatga
   113761 atgtcaaata taacatggat ttacagggtc tttcctatgg tcaaatactt aaaggagtcg
   113821 ggcgcgggtt gaaccctttt gacggtttta atgattttcg ctttgattca ccgcttgata
   113881 cgattcgcgg catagacgac tctcttattt ttaatcgaga ctattcctca accaatacat
   113941 ttgtgttatc ttatagtcct caaagtttta catggctcac ccatactatc aattataacg
   114001 ccgctaccgg aagcagtcgt aatagagcgg ctaactcaca actcaacaaa agcgcaacga
   114061 tacaaagatc ggtccagatt tcttccagct tcagtttgaa atcctttatc ggaagtgtta
   114121 aagagccgtt caaaaatatc gatacaagac aaccattatc cgcaacagag gattcgcgaa
   114181 aaaatagacg tgatcgtttc aaaaatccag gaagtgataa ttcggctaaa tcatctgaca
   114241 aggactccgg taatgtaatc actaaaggtt cttcattttt taaaacagca tctcttcgtg
   114301 ccggatcaac gtttgagaaa atacttttga tgataaatga cacccgtttt aatatagatt
   114361 ttaaaaattc ttttaacctt ggcgctttag aaaatcaatt agaccctgca gcaaaatggt
   114421 tcggatatat gagtaccgat aatagtggta tgttggatca tatttttaca tgggatttag
   114481 aacctattaa ccaatataga acacctgtgg attctgttct taaacaacaa gtaaagagtt
   114541 taactacaaa ctcgaaatct attaactatg gtttcatgta tggttttaat tttgggccat
   114601 ttacactcga cattaagtat gatcgttcgg aaactcggag tatttcaaca aataacacgc
   114661 aagatattag tatcagacgc tcttctttgt tcccatatct taaatttgtt ccttttcctt
   114721 tttatgaaat gacgttacgt gcatccaatg ttggtcggtg gcctatattt aatttattgg
   114781 ccggagcaac tcgtaatata aactttagca ttggctattt atcaaacgaa aatataactc
   114841 ttaatgaaat caggtcgcgt gtggatggaa ttatctttta taataatgac ggctttgttg
   114901 actccagtag gactatacaa aatatcggcg ggcgccgaat gcagacagta tctatggata
   114961 aatctataac ctttccacag ataagctttg atatttcgtg gaaaggtaat gtgacacaaa
   115021 acatatcctt ttcaaaatca aatacagaaa aattgaatca gaatacgaca atcaatcaaa
   115081 attctataaa tattttatct aatatatctt attctaaacg aggtggtttt cgtattccaa
   115141 tttggttttt aaagaaaaaa caacttgata atgaagtacg aatgtcttca caatttacat
   115201 ttagaaaaac ccaagaatat tcgcaatcaa acacacgctc gaatgatggt tttaggcagc
   115261 ccaaacaaaa aacatctgag acgacatatt ggtcggttga accaaggatt gactattcat
   115321 ttacacgttg ggtaacaggt ggaacttttt atagatatga atacaacaaa agtttaacat
   115381 tagggaaaac aacacgcgtt ctatttggat taaatgttaa tatcacgatc ggcgcttaaa
   115441 aattgatgta aaaccattgt tgatataaga aatactttat caacaattta tacctattaa
   115501 caagaaatta atacttacta ataatccaat aacatgtttg aaaatataaa cctttaaaat
   115561 acagaccaaa aaaaagttct tcacatatca acaaatctat actactacta ctactataat
   115621 aaataaataa gaataataga ggtatataat gtataaactc tcatttctaa ttgcgttact
   115681 gatttcctgt aacaatgaag tgaccaacaa acaaaacttg ttaaagatta aaatagaaag
   115741 aaaaaataaa gaaatatata cgcttttgaa tcaagaatct gaaacagaac gattaaatgc
   115801 agtccggtgt ttaggagaat ttcaggatag taatagtatt cttattatcg aagaaaaaat
   115861 aaagacggaa aaatctgatt tagtatataa tgcttattta tttgcattgg gacaattagg
   115921 ttttcttaat aatcataatt taaatttaag aatagaaaac tttcttatag aacaaattag
   115981 tcctaataaa gatacaccgt atcaattgta tgtggaagcg ttgagtaaag tagggtatca
   116041 aaattcaata ataaaaattt tagaaattgc aaaaattgat acaaacccaa ctttaaagaa
   116101 acatattaat ttgagtttag caagacttgc caatcgaaat caaaaaaata cagacgtatt
   116161 tacgtatctt aaagaaagta ttaccagtaa aaacgatgat gtacgatggt cagccgccta
   116221 tgcatatatg cgtatatctg atacaaatac agtaaagtat attttagact atgtaaaaga
   116281 tagtgatccg cgagttcgta tggatatagt acgatctctt gggaatatga agattagcaa
   116341 aaccaacgaa ttctataaaa caacgataaa cacattaatt gaaattctgc ataatgaccc
   116401 tgattggcgt gtccggtcca atgcagcctc aggtcttggg aattacaaat ttgatttcga
   116461 agaaattaag aagatttatt atttaacggc attagaaaaa acaaaagata aaaatgaaca
   116521 cgtaagaata agtgcaatac gatcgatagc aaaaagtttt tccggtgata taaaacctaa
   116581 caatgtattt cttaaggatt tttttgatcg ttatttaacg aatacagaaa aaaacatgga
   116641 aaacgcagaa atttttaacg cgctttgcct tatgtttgga aataagcttt tagaaaacgg
   116701 agactttatc gaatattgta aaaatgtgaa tcaaataaaa aacgcatatg ttagagctaa
   116761 aatatttgaa tcattatcta gtctcacatc tccagatttg acggtttatt ataaaacagc
   116821 attaaatgat tcatttttac ttgttcgata tcaagcgatt gagggcttgg gtaaaataaa
   116881 taatagtgaa gcaagggatt taatcgttca atctatcaat acgagcgacc ctacattatt
   116941 gtcaatcgtt gcaggaaaac ttgccgaaga tacggtatac cgaaaagatg acaggaagaa
   117001 agaggtgtta accaaaaaga tcatagaggc ttttgaatca attcaaaaac cttttgatac
   117061 ggaaggaaaa ataggtgtca tagatgcgct gatccagatt aaccataaaa gctcttatcc
   117121 tttcataagg acttttatca aagatcctga aaacaaaatt tctacatacg ttgccgaaca
   117181 acttgaaaaa attaccggtg tatcgtataa agattcattg ccaaaaacta atacaaataa
   117241 atctttagaa tataaaaaat tagatcaaat ttttgtaaaa aaaccaaaag cgaaaattca
   117301 tacatctaaa ggattaattg aactggtttt tcatgcaagt gaagcaccga taagtgtttt
   117361 aaattttgtt gaattagctg aaaaaaaata ttttgacggt gtttattttc atcgtgttgt
   117421 tcccaatttt gtaattcaaa ccggcgatcc gacgggaacc ggttggggtg gtccaggtta
   117481 ttcgatagta tcagagtata acacgctaca ttattcacga ggtgtagtcg gaatggcaag
   117541 cgcgggtaaa gataccgaag gctcacaatg gtttataact cattcagatc aaacgcatct
   117601 tgatgggaga tattctattt ttgctagcgt agagaacggg atggaagttg tagatcaaat
   117661 acaagtcggc gatcaagtaa aaaaaataga aataatatgg aatgaataaa acttattcat
   117721 tcaaattctt aacgaataac aaacaaacct gattatataa aaacatacca gcttgattta
   117781 aaaaaacaat atctgacgtg ttatttatat acggttttgc ttcggaaagg gctttttcaa
   117841 acatcgcgtc aaaagaaata ccgaacgcat ccaaaaaaat ttctttatta ataccctttc
   117901 tttgcctcaa gtttaaaaaa agaaactcgg ttattctttc atttatattt aaattctcct
   117961 cggcgtcaat tattttttgg ttttctattc ctgcgtaagc gatgtattta gacaggtcgc
   118021 gaacattcca ccatcgtcgc gagttatcga agctgtgtgc cgagggtcca aaacctaagt
   118081 atgataaccc attccaatac gatgagttat gttttgactc aaacccgtcc tttgcaaaat
   118141 tagatatttc gtactgagag taacctgatt tttcaaggta ctctgaaagc caagaaaaca
   118201 tttcaaattc tctttcatcg tcaagcagca caaccttttt tgcctttacc attttatgga
   118261 gatgggtttg ttcttctatg gtcaggttgt aaatcgaaat atgttcggga tccatttcta
   118321 ttataccagt aaccgtattt tgcagatttt ccagtgtttg tcccggcaag ccaaaaataa
   118381 catcaacact aatgttttta aaaccggctt ttctagccgt tacaaaagca cttacagctt
   118441 gctcaccatt atgagttcta tataaagact ttaattctgt atcattcaat gactgtattc
   118501 ccaaacttaa cctgttaatt cctgacctat taaattcatt taatttttcg gaattgacat
   118561 tgttggggtt gacttctatg gtaatttcgc aattttcact ttttataaag ttgccataaa
   118621 ttgaatttaa aatggatgta atttgaccat cctcaagaaa agagggggta ccgccgccta
   118681 aataaattgt acgagcttcc ggagcttcta attgagaggc aacataatgg atttctctgt
   118741 taatggattc gataaaagca ggtcggtatt tgcgtgacgt agtgacaaaa aaatcgcaat
   118801 aaatacacac tctctcgcaa aatggaatgt gcacatatat tccgaaactt ttgtttttca
   118861 aagctttatt actcattaaa ttgttattta cctcatgaaa ttagtttata tcaatagtgt
   118921 ttcaaagact aaaacgtaat tttaaaaaac ttgactttat ataagggctt aggtaaattt
   118981 tgaaacaatc aagaatagtc tttccttgcg ccgttctcaa taaaattaaa ttttaacaaa
   119041 ccaatgtgct ctaattttgc ataagttcct ctattcattt atttaactca aggagtgctg
   119101 tatgaaacgt tttttgggtc aagccatgat gatggcgttg atcgcgatgt tagctgtttc
   119161 gctaacaagc attgacgcga tggcacaaaa agacaaaaag aagaagaaaa agaaaaaaga
   119221 tggaccgccg gagtggattt ccaaaccggg tctgtatgaa gatgtgattg tcgcggcagg
   119281 tgtaggcgaa ggttttaatg aacaaaaggc taaaagccaa gctgaacaaa acggtcgtaa
   119341 aaagatcgcc gaagcgcttt caacagaagt aaaaagtctg acaaccaatt tcatggaaga
   119401 agcatcaaca acaacagaag agggttcctc aggagcagcg caggaatatt tttctgaagt
   119461 gactcaatcg atgaccaatc agacgttgag tggcgcgatg attgaagaat attggccgcc
   119521 aatgggcgag aaaaatggcg gaaaaataaa gttctacgcc aaggtagtgc tcaaaaaatc
   119581 agccgtagta gacgcgtata aaaaacaagt tgaagaagac atcgccaaaa agaaaatcaa
   119641 aagtgtcaaa ggttctgccg atgacgctct gaaagctctt gataatgcga tcggcaaatg
   119701 ggaaaaaacc aacgacaacg gtccttcaac cagcgaagaa ggcggcgcag aagaataaaa
   119761 gtaattttta gtaatgctaa ccgtagcttc gctttgatga ggctacggtt atttttttga
   119821 aatctgatag gagtaggtat gaaatcagtt caaatgtttt tgattagtac acttttggtt
   119881 atcatgtctt gtggtggcgg taaagcgaca tccaaaaatg aaaataaaat tcctaaatgg
   119941 gtcagcaaac ccgggcttta tgaggatgta attgtagcgg caggaatagg agaagggctt
   120001 tccgagcaaa aagcaaaaag ccaagccgaa caaaacgggc gcaaaaaaat agccgaagca
   120061 cttcagacac aagtcaaaag cctcacaacg aacttcatgg aagaagccgg aacaaccaca
   120121 gaaaaaggta ctgaatcggc tgcacaggaa tattttcagg aggttactca aacccttacc
   120181 aacattactc tcaacggcgc tgttttggaa gaatactggc caccgatggg agaaaaagac
   120241 ggaaacagaa tcaaatttta cgcaaaaatg gtgttaaaaa aatcatcgct cgtagatgaa
   120301 tttaagaaaa aggttgccga tgatattgca cagaagaaaa tcaaaaatgt aaaagccagt
   120361 gccgacgaag cgttaaaagc gcttgataaa gcgatcggta aatgggaaaa aacatcggaa
   120421 tctgcagatt agttaataat tattcgggct gtagccatta aataaaatga tacagcccgt
   120481 ttttttaaat tagtcttata caaatactgc gacataactt gattttgaga agctatttgg
   120541 aacacattac cacatttaag gttcttacat attcgatttc gcttcaagtt tgtatgaggg
   120601 caattatgga aatgcaatct aaaatcaaat ttctgtggct tgttattttt ctttgctttg
   120661 tacaaactaa tttatggtca caaggcccgg caccgcaatg ggtcaatgaa ttgccaatga
   120721 aagacggtac gttgtacgcc gtaggattgg tcaatcgtta ttatgaaaag aaagcgggag
   120781 aagaagccgc cgatagcgcc gccagatcgg agttatcaca aacgttgaaa ctaaatatta
   120841 aaacgacagt tcaaagttgg tccggaacca ttggtggtac aggtttcatt caagaattag
   120901 aaaaaacgat agatcaggat gttttcgaat ccgtgcgtga ggcacaagtg gtgggtcgtt
   120961 gggtggatcc ggaaggattg acttactctt tagtatccat gcctctttcg ggagtgagta
   121021 caattcaaaa taaaatagta gcgtttgcca aagaaaattc cggggggaaa aaagaagcaa
   121081 aaaaaataga aaaattagaa gtttctctca agggcttaaa agatcccaaa tcatatatac
   121141 gttcctcaaa accggattgg ataaacgggt tacccgagga agatgatgct atatacgcac
   121201 ttggcatagc tgaaaaattt tatttttatg ttaacgggcg tgaatcggcc aaagataaag
   121261 ccagagcaga aatagcggca actgtaaaaa cggaagtaaa tgctatacta acggattggt
   121321 atgaagttaa cgaaggtagc gcttcgtacg gatcccaaca aagttttatg gaagaaatgt
   121381 ccaatcaagt gtctgaagcg acactaagcg gtacgcaaat cgtagaaaca tggtatgaca
   121441 aatctaccaa atggcattat gcacttgctc gcatgtccct aagcaaagtt gtcggccaaa
   121501 tacaagataa agcaaaaagt aaagttaaag atgaaaaagc tctaaaaggg ttaagcgata
   121561 aactcggtaa aatgatcaat cgcgattatc tcaaatccaa agatggttta cccacgtgga
   121621 tcaataaact gcctaaggat aaagaagcaa tatttgcagt gggtattgac aacggaaaat
   121681 atttttctga aacacaaggt atagaaaaag cgaaagccga cgcaagaacc aaattggcta
   121741 agaccgtatc cgtgacagtg gaaaatatca caaagtcatg gattgagatc gaagaaaagt
   121801 cgggcgatac aaaaggcgaa ccactcaatg actatatgtc ctccttgact aaagaagcaa
   121861 cggatgtcag tttgcaagga tctcaggtac tatccgtata tacaaaacgt gataaagatg
   121921 gaaaagtaac atattatgcc ttaggaaggc tatttaccgg aagcatgatt tctaaattaa
   121981 agaaaaaagc atccgaagta atgaatatac cacaaacaaa acccaacgag actcaagaac
   122041 aaaaagcaaa acgaatactt agttcgggtc gagaaaaagc cgccgctgcc ctcgagcgat
   122101 tgaacgccgc attagataaa gtaaatcaat aatatatata aatactaccg gaggatatga
   122161 taatgaaacg ttgtatgtgg atgcttgcga tggcgcttat tgtggccgcc ttactcccta
   122221 tggatcaagc acaagctcag aagaaaaaat caaaagatga taaaaaatcg aaatccaaaa
   122281 aaggcggcac gccagaatgg gttaccaaac cgggcttata tgaagacgtt atagtcgctg
   122341 ccggcatagg cgaagcaatg tccgagcaag ccgcaaaaag tgaagcagaa acagatgcac
   122401 gaaaaaaaat cgctcaagtc ttacaaactc aggtgcaaag cctgacaacc aattttatgg
   122461 aggaggccaa tacaacgact gaggcgggat ctaccggcgc ggcacaggaa tattttagtg
   122521 aaataaccca aaccatgacg gaccttacgc tcaagaatgt aatcatagaa gaatattatc
   122581 cgccacaagg tgaaaaaaac ggaaaaaaaa tcaagttcta tgcaaaggct gtattgagca
   122641 aaacagattt tgcagataaa tttaaaaatc aggtttctac agatgtagca tctggtaaaa
   122701 taaatggcat taaaatgagc gccaacgacg cactagcccg tttgaataaa gtcttaggcg
   122761 gctggggtca ggatgaagaa atcagcagtg ctacagataa accaaccgcg acgacaggtt
   122821 tgggggctgg aataagcaaa cccggcggaa tcggagatac acctacgacg acggcacaaa
   122881 ccggaagtaa agccgccagt tatccagatt ggaaaaaaag cttacccaat cgtccatcgc
   122941 gtccaggata ttatcaaggt ttgggtgtag cgccaattac ttcaaatcaa gcacaagatg
   123001 aaaaagacgc tgaatcggaa gcgcgagctc aagtcattcg agcaatccgt tctgaaatca
   123061 ccagtaaagt aaccagcgcc atggaagagc gcagttcatc taccggaggg agttcctcgg
   123121 gaagctatag cgaaagcttt aattccgtaa cggaatcttt ttcaagcgaa tcacttaaaa
   123181 acctgatcgt tgaatttcat acggataaat catccaaaaa gcgttacgct tattgtgaga
   123241 tatccattga agaagtcaat cgccagtttg ctgaacgatt gaaaaaagcg atcaatgttg
   123301 ccaaaaccta ttatatggca gctaaatcat cggaagcaaa cggagattat tatatagcac
   123361 tcacacagta tcttgaaggc gcaaaagaag tggtcatagc ggaggcaatc aataaagaac
   123421 ccatcgaagg tgacattgac ggatccggca aaagtgtatc cgttcgagcg acatttgaca
   123481 cacaactcaa gcaattgctt gggcgtatgc gtgttgaaat cgtaagcggc gacgctcaaa
   123541 agggaacgaa aaatgaacct ttagcccaac ccttagtagc tcgacttgtg tatgacaaca
   123601 tgggcaatgt gatgtctgtc aaaggggctg ttttagtttc ctcatccgtg gctcctactg
   123661 tcgtaaaagc ggatttgtcc gtacaaacgg atggttccgg aaatgcagga tttacagtgc
   123721 acgccgtaga atcagttaat gcgagtggtg ttaataaagt tcgtatcggc cttagtacgt
   123781 ctgattttga agcttttgcg acccaacttc cgggtgcgct agaaaaagca aaaaccgtat
   123841 tcaaagattt tagtttttcg gcgcgcggtt catcggtatc ccgtatagct attttgattt
   123901 ttgaagaaaa cattggaaaa cctcaatcaa attcaattat cgaaggcgac atcgtcaaac
   123961 aattgcttgc aagcaaatat aaagtcattg ataaaaatga agtatatcgt gctatttctc
   124021 gcgaacaagc caaagcgtca gcagaatctg gcgatgatgc ggcggccaat gcgatcaaat
   124081 ctatggccga tgtattggtg atcggttatg ctaaagccat ggagtccgtt ggaggttcga
   124141 ccaatcctta cggcggtggt acgagtaatc gtgtatcggc gtgggctgac tgctccatac
   124201 gtgtagtgga cttggaaacc ggtaaagttt tagcatccag cgatattcag cgtgaaaaag
   124261 ggttgtctgt aggcgccgca gaaaaggcgg ggattatcgc gctccaaaat ctgagtaaaa
   124321 aagcatccaa agaaatagtt gataacatga atgcggcatt aaaataacga gtgttcatcg
   124381 ggagaagaaa tgaaatcatt tataattatc ggttgtttaa tatgctcact cgttgcttgt
   124441 ggcgaagtca aaaaaaagag cactgaaaaa acagaaaaaa caccggaaac cgttcaaccc
   124501 gaaagtgaat caccttcatc tgaagacaag ccgtcatcat tggaagaact ggtttatgaa
   124561 gcaacagggg catccaaact ccgtaccgaa tcggcggcac gcagcaatgc cgaattgaat
   124621 gggagaacag ccctaatcga aacgcttact gaagatggtg tagggttatt agaaaagttt
   124681 tatctcgaaa acaaaggcat gtttgatgca tctatggatt tggaaaaata taaatcagac
   124741 ataaaagctg tgtttaaaaa atcaacaacc cttaagggaa gtactgttgc ggaatatacg
   124801 caatccagaa aaggggatac gacgtatgcc atcgtcgaaa taccactgat gggcggatat
   124861 gaagccatcg aagaacagat cgtatccgta ggattaaaaa ataaattttt gaaatcagaa
   124921 cacgtagctc attttaaaac ggcttttaaa gagttttttt tgaacgagaa aaagaaaata
   124981 ctaacaactc cggcatagcg taccggctga atatcagagg atatcgcgag gatttttatg
   125041 agaaaatggt ttatttgttt agcgtttgta atgcttgtca atactgcaca agcccaaaaa
   125101 gaatccgatt ttcaaagcgg cgttgttgca tattccaatg gtcaatacga acaagccata
   125161 ggtttttttg aatcggcatt gaagtattat agcagtataa aaaagaaaaa agccgctgcc
   125221 gccgattgtt ataattatct ggggcttctg tatcaggtgt taaatcaaca cggcaaggcc
   125281 gtcgaaaatt ttaaagctgc cgtgccatta tttatcgaaa caaaaaataa atccggagcg
   125341 gcgagcgctt taggcaactg gggaatttca tcttatcgcc tcaagcttgc cgagttacgt
   125401 caaaacaagc agagtttttc tttagaagac attggttcgc tcatggcata tcacctcaaa
   125461 gcgaaacaat atcacgaaga attgaaagat aaagccggag ttgcaaacga tctcaacaat
   125521 atcgccacca tgtattttga aggtgagttt tacgatgaag catcagacta tctaaatcaa
   125581 gctttgaaaa ttcattctga gatcggttat aaagccggta tggcttacga tatggctaac
   125641 cttgcccgat tgtattacaa aaccgacgat gaaaccggcg cattaaagtt ttatcaacgc
   125701 agtgtcggga ttcttgaaga aataggagat cgcgaaggcg cttggcgcac ctattcatat
   125761 atcggttcga tctatgaaag ttttatgaaa caggctgcca ttttgaaaga cgcacaaaaa
   125821 gaatcagaga atagaaaatc agctatcgtt gcgctcaaaa aatcggtcga aatcatagag
   125881 tctttccgtg gaaattttac caataaagat ttttttgaca gctatctcaa agacaagaat
   125941 cccgtttaca aacgattaat tcgtttgctt aaactggatg ataatgcgtc agaagcgctt
   126001 tactacatcg agcgcagcaa agccaaaatg tccaatgatg ttttgagcgc cgggaaactc
   126061 gcatttaaca ataaagaaga acaaaagcag gttgataagg ttcaagatct gcaaaagaaa
   126121 accgaagaac tcgaaaagaa gattgccgat gaaaaagcca aaccggcgga taaacaagat
   126181 aaagcgcgca tcgacagtct tagcaagtca ttgaccaaaa atcaaggcga ctttaatgct
   126241 ctgatgattg atttggaaac gaagtacccc aatatttatc aagtgatcaa agtagatccg
   126301 atacagttaa gcgatattca aaagcaactc tccgacgatg tagtaatttt agaatatttt
   126361 ccggcggacg acgcgctcta tatttttatg atcaccaaag ataaaatcga ggccaaatca
   126421 gttcccgtat catccacgac gattgattca cttgttaaca aatatcgcta ctatattaac
   126481 gatgttactt ctttgatgaa gcgtggccgt tttgacacca aattagcaaa ttggaaacaa
   126541 ggtgaagact cacgttttta cgaacgtcat accaaaccgc tgcgcgacat tatggtaaaa
   126601 ttgaatgact atttgatttt accagtttgg gaaaccatca aggacgcaaa atataaaaac
   126661 gtaaccgtaa ttccggctgc aagtttgtat tatgttccgt tccagtgttt ggccactgaa
   126721 acgcaggatg gcgacatatc ctttttggta gagaaaaaag ccgtgaatta tcttacggca
   126781 gcaacactga tggatatgat caacaagaaa aagcagaaca aaatcaataa tgttcttgtt
   126841 ttcggaaatc cggacggaag cttaccagga gcagaagaag aagcgaaaat catcaaagcg
   126901 gcttatacgg atgccttact atacaaaaat caagatgcaa caaaagataa agccaaagat
   126961 ctttccggta ataccgaagt tgtacatttt gcaacacacg gatttttatc cagcgatgaa
   127021 ccgcaaaaat cgtttttatt gatggcgcct aatgaagcaa aaaatgaaga cgataaactg
   127081 actataagtg aaattctcaa aatgccttta aaagaaaaaa atgaactggt cgttttatcg
   127141 gcatgtaata cctctatggg taaaagcgca acaggtgtag aactcataag cctttcgcgt
   127201 gcgtttgcga ttgccggtgc accgacgacg gttgcaacat tgtggccggt agatgatgaa
   127261 tcaactaaaa taatcatggt caatttttat gatggattga aaaaaggatt ggccaaatca
   127321 gaagctatgc gacaagcgca aatcgcatta atccgtaaag gtgattacca tatccacccg
   127381 tttttctggg caccctacgt catgatcggc aaccccaaat aattaaacac aatcaatgat
   127441 aaaaatcccc gtttgcattt gcaggcgggg gtttttatta tgtatgagca attcaaaaaa
   127501 gccgcaaacc atgtttgggt ttttctcaaa aaaagcaaaa aaaacaatcc gagaggatga
   127561 tgttatttgg caagctgaag atataaagat acataatctg attttgcgct tggccgatta
   127621 ttctcataac tcagatggta ttatgatcat cgctcacttt ccaaaaactt tagagcaact
   127681 catagtatca gccaaaaaaa ataatttagt tttaaaagaa ttgtatacaa taaccgatgc
   127741 cgatcgttgg gcgaccggtt attataatga tcctgtggcg atcttatcaa aagcaatctt
   127801 tgtgtcttca agccaaccac ctatggtgca ggaagaaaag aagaaaaaaa tcattgtagc
   127861 agagcatttt cctacaccgg aaaaagacat tttgctaatg aattgggcga taaacgtgaa
   127921 agcagaaagc attcaatggt atgaatctac ggacaacccg attttcactc gatttggtgg
   127981 tgatcaaata agaattttag ccgaaaaatt gggttggcaa caagatgaat cgatgcaaca
   128041 tcggtttatt acctcatcca taggttctat gcaagaaaag atatttcgca aatctaccgg
   128101 agacggggat gctgaagacg tcatgcaatg gtttaaatcg tatgttccat catcgttata
   128161 aaatatttta ggaggtaaat catgggtcag ctcaccgctt ttaattttaa aaaatggata
   128221 aatgagcatc gtcatctcct caaaccgcca gtcggaaatc aggtggtttg gaaagacacg
   128281 gattttataa tcatggtcgt cggtggacct aacgcgcgta aagattatca ttataatgaa
   128341 ggcgaagaat tctattatca attggaaggt aacatcacac tgaaaatcat cgaagacggt
   128401 aaaccacgtg atgtcaaaat caatcaagga gatatttttc ttctgccacc tcgaacccct
   128461 cattcaccgc aaagaccggc gaatacagtc ggtttggtca tcgaaaaaaa acgcgccacg
   128521 gaagaaaaag acggatgtat atggttttgt gaaaaatgcg gcacgaagct ttatgaggaa
   128581 tatttttatc tcaccaatat tgtaacacaa cttccgccgg tatttgatcg gttttatgga
   128641 aatccggagc attgtacatg caaaaattgt ggtaccaaaa tggaacgtcc ggtattgcct
   128701 gtggcaaaag aaaaagcaaa atctaaaaga caaaaaacca aaaaaaataa cgtcataaag
   128761 aagaaatcaa aaaaacgtat caaacctgta aagaggaaat aatacgaaaa ttttatgctt
   128821 ctggatcaag ttttacctga ctatcatgtt cgggcacaat atcaaatttt gattccatgc
   128881 aaacgcgatg tcgtttggaa gacgcttatg gatatggatt tgaccagtat tcaaactctt
   128941 cgattgctta tggggttacg aagcataccg gcgtggctga tgggtaaatc aaaaccgcag
   129001 ttgaaaaaac atactctgca tgacctgagt gattcgggaa tgcatgtgat ttcagaagtt
   129061 actggctctg aaattgtact gggcataaac ggaaagtttt ggcaattaag cgccaatgta
   129121 agaatggaca tacctgcgga acgatttgtt tcattttcag aaaacgattt ggctaaagcc
   129181 gcctggaatt tttttctctc agaaacgaat ggtattacga cggttcagac agaaacacga
   129241 atattttgta gcgatccaaa aatgcgacgc aaattcagat tatattggac attgatcggg
   129301 ccatttagcg gtttgattcg cattgctctt ttgagaaaac ttcggtcaaa cactattcgt
   129361 tacgagaaaa aaaatggtga taaggggtac gaaaaaaaat agctatatga taaaaataga
   129421 tattcatact catattctgc ccgagaaact tccggatttt gccaaaaaat ttggatatgg
   129481 cggttttgtg tctttggaac atcacaaacc gtcgtgtgcc agaatgatcg ttgacggtaa
   129541 gttttttcgc gagatagagt ccaactgttg ggatccgcat gtacgcataa aagagagtgc
   129601 cgggcatggt ataaatatgc aggttttatc aaccattccg gttatgttta gttattggtc
   129661 aaaaccgcaa gatggtttgg atgtttctat gtttctaaat gaccatattg cagaaatagt
   129721 tagacagttt cctcagcgct ttagtggatt gggtacggtt cctttgcaag cgccggattt
   129781 ggcaatcgct gaattagagc gatgtatgaa aatcgggctt cagggcgtac agatcggatc
   129841 tcacgtcaat gagtggaatc ttaacgatga atggttgttt ccttttttcc aagcagcaga
   129901 aaaaatgggc gctgcgattt ttgtacaccc ctgggatatg atgggtcagg atcggatgtc
   129961 caagtattgg ttgccgtggc tcgttgggat gccggccgaa acttcgctcg cgatctgttc
   130021 gatgattttt ggcggcgttt ttgaacgctt acccaatttg cgtgtggcat ttgcacacgg
   130081 tggagggtct tttccttcga cgatcggacg aatcgaacac gggtttcatt gtcgtcccga
   130141 tcttgtagct gtagataaca aacggaatcc ccgcgaatat ctcgggaaat tttattttga
   130201 ttccttagta catgatgcgc gcatgttgcg ttatctgatc gaacttgtcg gagaaaaaag
   130261 aatcgctttg gggtccgatt atccattccc gctcggtgaa gaaattccgg gatcattagt
   130321 cgaatccatg gacgacttaa ctgaaaatat aaaagaacga atactaagtg gtaccgcagc
   130381 cgagtggctt aatatcaatc ttgaaactct acaaaaataa atgatgcaga tatttattaa
   130441 ttgtaatgat gcggaatacg ctatagacat taacagcccg ttggatattt caatacccgt
   130501 atattttgac gacttgcaac ctaataccta tggcgttcct gccgcatctg caagagctta
   130561 tgaagacggg aaatttgtag gtgacgtacg aagaggcgga agttgcaatt ttgaagaata
   130621 ccgcatcata ccgcattgca atggtacaca taccgaatgt gtggggcata tcagtaaaga
   130681 acgtattgca atcaacacct attttgatag acttttgatc cctgcaacac tcattacgat
   130741 cgaaccggaa aaagctgtgg attcgaccga gacatatgca ccggataagc agaacgcgga
   130801 tcttatcatc acagctaaaa gtctttttta taaacttcat actttgcctc gtgaatttat
   130861 gtcggccctt gttattcgga caacgccaaa cgttccatcc aaaaaatcgc gcgattatat
   130921 gaaggagaac tctccatttt tttctctaga ggcaatggat tatctttttg agatcggtgt
   130981 agagcatcta ctagtggata cgccttcgat agatcgttta tttgatgaag gcaagctgtc
   131041 agcgcatcat gttttttggg gagtgcctca aggatcacac gatattcacc tggaggtgca
   131101 ttcacgcaaa accattaccg aaatgatata tgttgataat agtattgccg acggtaatta
   131161 tttcataaac atacagattc ctaactttgt tgctgatgcg gctccaagtc gtattttgat
   131221 gtatcaggcg caactaaaaa atcaattact accataaaat atgaattttg aaaacagcgt
   131281 tggttttgct caaaaattag atcaagaaga ttctttaaaa gaatttcgtt cacggttcca
   131341 tattccctgt cataaggacg gaactcaatc catttatttt tgcggtaact cgcttggcct
   131401 tcaacccaaa tccgtgcgtt cttatatcga gcaagagctc aaagattggg aaaccctcgg
   131461 agttgaaggg cattttcatg ccaaaaatcc gtggatgcct tatcatgaat ttttgactgc
   131521 aaaagcggca aaaatagtcg gcgccaatga gcacgaagtg gcgcatatga attcgctaac
   131581 gactaatttg catttattga tggtttcgtt ttacaggccg accaaagaaa gacataaaat
   131641 actgatagaa aaaacagcgt ttccatccga tcaatacgcc gtacaatcac agattcgcta
   131701 tcatggttat gatcctaaaa cagcgttagt agaaataatg ccaagagatg gcgaagaaat
   131761 tctacgaaca gatgatattg aagagtatat tcaatctcac ggacaagaga tagctttgat
   131821 tttattgggt ggagttaatt actacacagg acaggccatg gatatgctac gcattacagc
   131881 agcagggcat aatgccggtt gtgtagtagg atttgatttg gcacatgctg caggtaatat
   131941 tattttgagt ttacacgatt ggaacgtgga ttttgcggca tggtgttcct ataaatatct
   132001 gaattccggt cccggaggaa tatcaggaat atacatacac gaaaaccata cacgtaatca
   132061 caacatacct cgctttgcgg gatggtgggg gcatgataaa aaaacaagat tcctaatgaa
   132121 acctgatttt ataccaatcc caacggcgga atcttggcaa ctaagtaatg cgcccgtgtt
   132181 attgatggct tcattgcgtg catctttgga tatattcgat gaagcgagta tggatcgcct
   132241 aagaaaaaag agccttcttc ttacaggtta tcttgaatat ttgattcatg atataaaaac
   132301 cgataatttt gtgttgatca ctccatcgga ttcttctcag cgcggctgtc aaatttcaat
   132361 acaaaccaaa cgaaacggaa aaaagctatt tgaaaaactg agtgaagcag ggataatcgc
   132421 agactggcgt gaacctgatg ttgttcgagt tgcaccggtg ccgctgtata attcctttct
   132481 tgatgtctat agatttgctg aaattttgaa aaagcactcc taattaataa aaggcaaatt
   132541 agtatgaaaa accttatcta tgcagcgctg tgggtcatta ttttggtttt gtcttgtcgt
   132601 caaccacaaa cccatcaacc gttatcagcg aaagacggtg taacaataga ttatagagta
   132661 gtcggtcagg gagatacgac aatcctgttt cttcatggat ggggtataaa tcagacctac
   132721 tgggatcagc aaatagaaca ttttagaaaa aaatataagg tggtaacttt agatttgccg
   132781 ggttttggca aatccggaaa aaatcgtgaa gcatggacta ttgaaaatta cgctgaagac
   132841 gttgctcacg cgattaaaac attggaacta caaagcgtta tactcgtagg acattcgatg
   132901 tcgggtagta tagttgtcga aacagccgtt cgtttgcctt caaacatcat tggtgttatc
   132961 ggagtggata attttaaaga tgttggtcac gtttccttgc cggaagaaaa ggccggtata
   133021 gatacattta tacactattt gggtgtagac tttgtgggga cgatcacagc atccgcagaa
   133081 ggaacattat attcagcaca aactgattcg acgattcgca agcggatttt gaatgatgtt
   133141 atcactggaa actcaattgt ttcaaaaaat gtattgaacg ctgttttttc atatggttca
   133201 aaagaagttg agatgttaaa aaaactgccg tataaattgt ttttgataaa cagtgataac
   133261 cagccgacgg agacgaaggt actttctcaa atagtcactc attcttgtga ggtgtttact
   133321 gttcacgcga ccagtcatta cccgatgatt gagaatgcag aagattttaa ccgacaactg
   133381 gaaacaatta tctcaaaatt aaaataatac tatgggcaac aacattacca tcgtcggcgc
   133441 cggattggcg ggttcacttt tatcggttta ccttgcgaag cggggttata ctgtagattt
   133501 atacgaacga cgacccgata tgcgccaagt caaaataagc gccgggcgtt ccatcaatct
   133561 cgctttatct acacgaggta ttcacgcgct taaagaagtc gggttatttg atgaaatcag
   133621 taaaatatta attccgatga aaggacgaat gatgcatggt accgatggtg cacaaacctt
   133681 tgtgccctat ggtaaagatg atacggaagt gatcaactcc gtatcacgag gcgaactcaa
   133741 tatgcgtttg atgtctttgg cggaatcgtt tcccggcgtg aaaatccatt ttaatcagcg
   133801 ttgcacgggg atgaattttg aaacaggcga agtttttttt acgaatgaat ccaacggtga
   133861 aaaacacagc attttttcag aacgagttat cggcaccgat ggatccgcct ctgcccttcg
   133921 gtctgatatg atgcgcctaa atagatttaa ttattctcaa tcctatcttg agcatgccta
   133981 caaagagcta actatacctc cagccaaaaa cggttcgttt cgacttgaga aaaacgcgct
   134041 acatatttgg ccacgcaaaa cgtttatgct gatcgcactt cccaacatag atgcgagttt
   134101 tacttgtaca ttgtttttat cgctggaagg gccggaaagt ttttcaactt tggatacgcc
   134161 agaaaaagtg actttatttt ttaaaaaata tttttccgat gcattagagc aaatgccgga
   134221 tttaacgaat cacttttttg aaaatccgac cagttctctg atgactgtca aaagtttccc
   134281 gtggagctat gaagataaag tcgtacttct gggtgacgca tcgcatgcga ttgttccgtt
   134341 ttttggtcaa gggatgaatt gttctttcga ggactgttct gaactaaatc gttttatagg
   134401 cacttattcg ggagattgga aaaagatttt caaagaattt gaaaaatcgc gcaaaccaaa
   134461 ttccgatgcg atcgccgatc ttgcggtcga gaatttttat gagatgcgtg atttagtcgc
   134521 ggaccctcgg tttctattga agaaaaaagt tgaacatata ttagaggaga aattttcgga
   134581 cttttttgtt cctaaatatg caatggttac atttcaccgt attccgtatt ctgttgctat
   134641 ggaacgagga aaaattcagg atattatttt ggatgaaata actactggca ttcatgaccc
   134701 tcatcatgtt gatatcgcca ttgcagaaca cttgatcaaa acaagactaa ctccctggtc
   134761 ggatgtagtt aggctctaga aaaattttca aactattaaa gtttatcgtt tagcttaata
   134821 ttttaccact tgcctgttga cttactgtta acttgattaa caggaacaac ctcccatcgt
   134881 atcatacaaa ctcccgcgcg ttacatagcg aactacattc ctcattaaag agccaatcac
   134941 taatttgaag gttagggaaa gaaacaaata tccaaaaaat ccgtgtaaga ttttaatttg
   135001 atgaaaatgg agagaatgta tgagtacagc acgcttggcc ggatttttat atttagtggt
   135061 agtgttgacg ggaatgttta ccttagccta tgtgccctct gtactcattg attggaataa
   135121 tgcctcggtt acttataaca atataaaaag cgcagaaacg ctatttcggt taggtattgt
   135181 ggccggaata atatgctaca cagcttttgt attgttaccg tttgtactct ataaactact
   135241 gagccaagta aatcaaacat ttgccgttgg tatggtggtg ttatctacgg taagcgtccc
   135301 cttttctctc gtaaatcttt tgtataaggt caacgtctta actctgatcg caaattcagg
   135361 aaatttaaca gaccctgaac tggaaaaaac acaagagcag attatgttac tgcttaacta
   135421 ctatggtaac ggaattcaaa tcgtttccat cttttgggga ttatggcttt ttccatttgg
   135481 gtatttggtt tttaagtccg gctttcttcc gaaaatcctt ggtgtttttc taatggcggg
   135541 ttgttttggg taccttatca attttacagg ccgctttctg tatccaaatt atgccagttt
   135601 tggaattggc ggatatgttt cacttccggc gactatcgga gagataggaa tatgcctttg
   135661 gttgttgatt ggcggaatca aaacgcaaac agcatcagaa taaatatcca gacgttcgta
   135721 agcctttatt aaatcatgat aacacgttta tttgaaaata tatttataaa taaaaacaac
   135781 aattcaaatc taaagcgatg aagttgaaac aatgatcaaa ataaaagaac atgatatcaa
   135841 tatcagattt aaacttacag cattgtggac atccgtaacc ctttgttact tgtattgtga
   135901 ttattttcag ttgtacgcgc cagaaaaagt caaaggtctc atgagcggtc aaaacatgct
   135961 tgatagtccg gtaaagctat taacagcagc agtgatgatg gccgttccgg cttgtatggt
   136021 atttctaaat cttgttttgt cggatcggat tttgaaggtt ttgaatattg ttttcggttc
   136081 tttttttact ctgatcatgc ttcttattat accggctata ctaaaacctt ggtatgcgtt
   136141 ttatgtgttt tatgctgtgc tggaaatcgt cattacaata tttattgttc gaatggcatg
   136201 gaagtgggat atcaacaaac cggctaaagt aaattgacgc tgaaatcaaa aaatatttca
   136261 aatctatttt ttggttggtt tacttttaaa atatcccgcc tacttttgaa tcaattctag
   136321 tgagtctctt taataagatt taatcgattt ttgaagttga tacatcaaga tcagttaaat
   136381 gtttatcaat atcttcgatc tttgacttta gtcatacttt ttatagattt tagtgctttc
   136441 cttttacagg cttttgatat tgtcctttcg cattagagcc gaaatcgtac accactttta
   136501 ggagacatgc ggtatgaaga ccttcacgcc tgataagatt cgtaatattt gtttggcatc
   136561 tcacagcggc acaggtaaaa cgacgttggc cgaggccatg ttgtttgtat cgggcaccaa
   136621 aaaaagattt ggtagtgtgg atgagggctc gacatccagt gattttcacc cggatgaaat
   136681 cgaacgtaaa ttttctatca atacgtcttt aatgcattgc gaatggaaag gccataagat
   136741 caatattcta gatacgccgg gttatgctga ttttcgaggt gaggtgcgat cagctatgcg
   136801 tgtcgccgac gtggtgatta tccctatacg tgcgacggcc atgctggacg tcggcgctga
   136861 aatcgcatgg aactatgctg aagaacacaa tctgcctgtg attttttgtg taaacgaatt
   136921 ggataaagaa aattccaatt ttgatcagac ttttgatgtc ttgatggaac gcttcggtga
   136981 cgggcggcat atcgtagaga ttggttttcc tattggcgag gggcgccctg attttaataa
   137041 aatttttgac atcatcagca tgaaactgct gaaatttgag cttgataaaa ccggtaatta
   137101 tgaagctctg gatgtaccga gcgaatttaa agagcggcta aacaaactgc atgaaattct
   137161 tatcgaagat gcggccgaga gcgaagaaga atttatggag gatttttttg caaccggtac
   137221 attgacagac gaagaattca aaaaaggatt acgtgaaggt attcagggca gaaaaatcgt
   137281 acccgtattt tgtacagatg gcattaattc ggttggcatc aaaccgttat tggattttat
   137341 agtggattat tgcccttctc cggcggacat gccttccgct accggtcgca ccaataccaa
   137401 tgctcaggag caacgtccta ataaatcaga tgctcccttt tcagcgttgg tttttaaaac
   137461 aattagtgaa aaccatgtcg gtgaactttc acttttccgt gtatattccg gtacggtcaa
   137521 gtcgggtgac gatgtactca atgccagtaa aaacgtaaca gaacgcatcg ggcaagtttt
   137581 cttactaaac ggaaaagagc gtgaagacgc cggacaggtg ggtgcgggcg atatagctgc
   137641 gcttgtaaaa ctgaaagaca ctcataccaa taatacgttg tgtgataagt ctaaacccat
   137701 cgttttcgga gaaattgcat ttccggatcc ggtaattact tttggtattg tgccaaaagg
   137761 aaaagctgat gaaaccaaaa taagccaggg tttacataca cttcacgaag aagacccggc
   137821 atttctttat acctacgacg ttgaaacgca acaaagcacg gtcagtggcc aaggtgaaat
   137881 gcacttgacg atcatcaccc gccgattgaa agaacgtttt ggtgtcgagg tggatttggt
   137941 tgacccgaag attccttata aagaaaccat tcggggggca gtcaaagacg ctgaatttaa
   138001 acataaaaaa caatccggtg gtcgcggcca atacgggcat gtacacatca aactttcacc
   138061 caaacaacgc ggtagcgggt ttgaatttat tgatgaagtg gttggtggtg ttgttccggg
   138121 gcgctttatc ccggcggtag aaaaaggtgt ggtcgaagcg atgaccggag gtgtcgtggc
   138181 gggttacccg gtcgtggatg tagcggttac tttgtatgat ggttcatacc acgatgtaga
   138241 ttctgatgaa ctttccttca aaattgcagg tgctcaagca tttagaaagg gtttcaaaga
   138301 aggtcatccg attattttag agccgatata tgaagtggag ataaccattc ctgaagaata
   138361 tcttggtgcg attatgggtg acatttcttc acgtaggggg caaattttgg gaatggaagc
   138421 cgacggccac ttccagatcg tcaaagcgca attaccttta gcagaaatcc ttaaatatgc
   138481 tacaatattg cgctctatga ctagtggtcg tggtatgttc cgtaaaaaat ttgaccatta
   138541 cgccgaagtt cctcatgaac aggccgctaa acttatgaaa gagtatgagg aacgtaaagc
   138601 tgccggaaac ggataaatat ttataatcag tatagtatcc aaagccgcgt cagaagtatc
   138661 aatttatatt gctttcattg tgatatataa ttagagatat ttctgaggcg gctttttgtc
   138721 tttacaagat aagtatcggg tttattgatt taaatcaatg aataatgata gttttaaccc
   138781 gatcttcagc ttgatattga agatcggcat caaaaaagat tttttttagc gagtgttgat
   138841 aagaacatat caatttttga tttacctttt attgtttgtt tgtatttctg tcgtatcgta
   138901 ataactcgag aattgacgaa acttaaaaga cataataacg tgtaagtagc aggtttttgc
   138961 gaagttcttt atctcatttt gaatataaaa cccatttaat catttttatt ttgtataaag
   139021 ttaaattgtt taggagtcca atgttacgtt gtattactat cttatggatg ataggatatt
   139081 ttcattcggg aacggtatca tctcaggaat atggttcgat caatggtttt gtatatgatg
   139141 cagccaatgg ggaggctgtg atcggtgcta atgtttttat caatggaacc aaattaggtg
   139201 ccgtaaccaa cctcaacgga tattatgtta ttcccaaggt tcctgccggc atttatttat
   139261 tagtcgttca atctttgggt tacaaagact acacgcaaaa gatcacagtc aagcccggtg
   139321 acaaacttcg tgtaaatact cagattcatg gcgaagatat tcagggtgaa gaaatagtgg
   139381 ttacggcgga ctctatacct accattatga agttatttga ggagcctatt tcacgaattg
   139441 agttaaccgg tacacaaatc aatcaaatcc cgcaagtcgc cgaatcggat cttttgaggt
   139501 ctttacagac cttgcccggt atccttccag tatccgactt ttcatccgaa ctttatgttc
   139561 gaggggggac gccggatcaa aatttatatt tattggacgg tacggatgta tataacccgg
   139621 aacatgcctt tggtttgttt tcaaccttta ataccgatgc gatcaaacaa gtgcagattt
   139681 caaaaggcgg gttcggtgct gaatatggcg ggcgattatc atccatactt aatgttacaa
   139741 accttgatgg aaaccgaaaa gaatttgaag gtagtgccgg tattagcctg ctttctgcaa
   139801 aaacgacgct tcaaatgccg attggtaatt ttggatcggt atccgcttcc atacggagaa
   139861 catattttga caaaacactt ggccaagcta ttgatgaaat ccctaactac tacttttatg
   139921 atggtaatat caaagccttt tttgaactgg atagccgaaa cacgcttacg ataagcacat
   139981 acggcggacg ggatgttctc aattttaatt ttaatgaaaa cgccgaagat aagtcgggta
   140041 tagattatga ctggggcaat aaaaccggca gcatccgttg gacacgggta tttaatccca
   140101 attggtttgg caatttttgg gtcacaggga gtgtgtttag ttcacaattt catcttgatg
   140161 agattgtaga tgaagacaac tttataagtg atatcacaat aaaggggaat atcgaatacc
   140221 atcaatccaa acaattcata tataaattgg gtttcgaaca aaaaaccatg cacgggaaat
   140281 atcagcaaga ttttcccaat ggtgccgtag atgtagatgc gcatcgctat ctctatacaa
   140341 tgtatggcca gacgaactgg cggcccaatg aaaattggga tatcgaagcg ggtctaaggt
   140401 atgattattt tgattcaccc agagattatc agtttataga tccgcgattg gcggctaaat
   140461 atcgtattaa tgaaactacg aacctcaaag cgtcggccgg tcgttatcat cagtacgtgc
   140521 atcgcatacc gagaactttt tttgccagca tatggactac caccgatcaa tatcaaaaag
   140581 catcagaggc ataccacttt ataaccggat ttgaaaaaac ctttgacgat gtttatcaag
   140641 ttgaagtaga gacctattat aaaaaatata atagtattta tacgcttaac cgttttttgg
   140701 tgacggatat gaagccgtct tattatgatg aacaaggcaa tcctattttt acgaatacca
   140761 acggtatttt taatcacgga aacggtaatt cgatcggtct tgaattgatg cttagaaaag
   140821 acgtcggtgc tttcactgga tgggtgggat attcgctggc cagaacggaa tataatgtgg
   140881 aaggaaaaaa tcacggtcag tcgtttacac cgcgacatga ccgtacacat acgatcaatg
   140941 cggttggaaa catagacatt cgtaacggta tgagagttat ccggggtctt gagactacca
   141001 aatcaaacag tcggtggact cttggtttta attttattta ttcgtccggg caacctataa
   141061 cccgcccagg ctcgctttat gtcataaccc caacgccgga caacggtaat cctgttatta
   141121 tcgaatatcc ggatgatata aataattacc gtttgcccta ttatgcacgt ttggacgtga
   141181 gtttgaccta tgaaaaacag tttaaatcgt ggtctatcgc tccgtatttg caaatttaca
   141241 acatcggaaa ccgaaaaaat gtttggttta tagactatcc gtatgaaata aaagagggcg
   141301 gcacgataaa acccaaagta aaaacaacct ccatgtttcc catgcttccc agcatcggta
   141361 tcaattttac attttaagaa ggcatatata tgaataagtt taagttttta ttttatatac
   141421 cattattact gattacggct tgcggtgagg gtaaagtttc aatagataac atcaactata
   141481 aacctaagat cgttatcagg ggattgattt ttccggggca acccgtttcc ggtataagta
   141541 taactcgcaa tctgccaata cgtgaacaac aattgacatt ggatcaactc atcatcgcag
   141601 atgccaaagt cgtaatcaca aacgtatcgt ccacggttac agatacactc gtatttgatc
   141661 cgattacggc gacttattat gatccggaac acaatttttc cgttcaatat aatacaatct
   141721 atagactaaa tgttgcagct cagatagacg gtaagaatct gaacgcgagc tccgaaacga
   141781 aagttccgca gcagggtttt agtatcattg aagaaagttc ccgattagat agtttgcact
   141841 accgggagag ggatgaaaaa gacagtatcc gcacatttac agttcgtttt gagcgatccg
   141901 tcaatgcaga aacttatctt atgtcaatga atgccttgga tgcggatact tctacatacg
   141961 tttattcgcc ggtcaaccct tattccgatc taaaaccttc ggatgttttg aaagatttgg
   142021 atgattttaa atattccacc caatggagct cactttttga aaaccaaccc ggtcaatcag
   142081 aaatgagcgt attttggttt ttcctatcgt tttatgggag atatcagatg gtcgtgtatg
   142141 cagcggatga gaattattca gattttatca acacctacga tcaggtacaa gatattgatg
   142201 gtaactacga tgaaccggtg tttcacattg acggagatgg gatcggagtg tttggttcgg
   142261 ctattgctga tacggtatat tttaaagtat tgaagccata agaaggatat ataaaaaaca
   142321 tatacctagg attgttactt ggagcgattt ttatataaca cataatatcc tgcgcccaat
   142381 aaaccggcag tatttcctaa ctttgcccgt actatttgaa gttttggctt aataggtttc
   142441 ataactcttt tgatgacttc attatgaata cggcttacaa acgcgtcgcc ggcttccgat
   142501 agacctccgc cgatgattat tatatcgggg ttgagaacat gaactatgct cgacaacccg
   142561 gctccgagat aaaaaaccat ttgttctaaa atgtttaggc aaagcgtgtc gttcttttga
   142621 gcaaaatcaa aaatagtctt cggggtaata ttttctaccg aagacagcac ggacttatcg
   142681 ccacgagcaa tagcagataa agcctctttc acgatatatt taccgcctgc atattgctcc
   142741 aaacatcctt ggttaccgca accacatggg cggccgtgtg cttcgacgat cgtatgaccc
   142801 aattctgcac cattgtatga ggagccgtga aatatatttc cgtttatgat aattccaccg
   142861 ccaacacccg ttcctaacgt aatacctacg accaatttat attgttttcc cgctccaagc
   142921 atcgcttcgc cgaatgccat aaaattagca tcattatcag caaaaacagg aaggttcatt
   142981 ttttgtgaaa tatattcagc aatcggtgca ttttcccaat cggggagatg cggggttggt
   143041 ccaagcgaaa tcccacggtc gctgtcaata gttccagggc atcccactcc gattccttcg
   143101 acttcaaatg attctttcct accgaacatc acacattcat aagcggcatc caccatggtt
   143161 tgcagtacat ggtcgcgtcc atgatcggca aatacaggta ttacattttg atatatgacc
   143221 tcgccgcggt cggagacgat gccatatttt aagttggtgc cacctaagtc caaacctacc
   143281 gcatagcggc gcggtttatc ttcatgtaac aatcggtcat tgagcgaaat catattgccg
   143341 gataggaata atgctgagtt tgtgccggtg agtgcgtaca aaacgttgta tagtcatgcg
   143401 ttcatctaaa atttcgggag tttcgatcaa accgtttgtt gatatcgtcg acagagcttc
   143461 cagtaggttg gggttttcaa gttcttcgcc gtgtaatacc ttagacaaaa ggccgttatg
   143521 aatgaaatag ccgacacgat cttccttttg gattaacaaa aacttttcat caaaagcatc
   143581 cgggttatca tccatatgtc tgcgaacgcg ccgtagcgct tttctaaact cgaccaactg
   143641 cggagagcgg gcatcacgcg attttaaaaa ggaatcgatg ttttctttac tttcgatacg
   143701 aatgtattca gcgaggacgg ccatggcatc ttcgacttgt tcacggtatt cttcggcgga
   143761 gatggcttca ccacaagcgc cgctgcactg acggagctgg tgcgcaaaac aaaatccttt
   143821 cggtgaacgc cgttccttca ttaattcagg gcataaacga tagtgccttt gcagtttgcg
   143881 caacatatga tcaagaaact taccggagcg aaacggtcct atataatgac ccccgtccgc
   143941 ttttttttct gctacctgaa aaactctcgg atatggatcc ggcgttactt tgatgaataa
   144001 gtaccctttt cgggcacgtt gctggatatt ataaggcggt tgatgttgtt ttataagacg
   144061 ggattctaat atgagtgctt cgagttcgga ggaacatgga gtaaagtcaa aatgatcggt
   144121 atatttcatc aaccgacgtt ttcgaggaat gtcaaattca cgtacatggc cgagaatacg
   144181 tgagcgtatg tggttacttt taccgatata tagcggcaaa tcatgtgtat ctttgaacat
   144241 atacacgccg cgcacgttgg ggagtttgaa taactcgtgt tctaaacgtt ctagctgctt
   144301 atcccggcgg tcaaattgtt cggcgtattt tgaaaaaatc tcttcggcgt taggcatttt
   144361 aagaaaccgt atcaaagctt ctgctgttgc ttgcgcgtca tctagagccc ggtgccttgc
   144421 tttgaccggt atttgaaaat attcgatcaa agcgccgaga ttatgtttgg tattttgggg
   144481 aagcattttt cgagagaggt ttaccgtaca aaaccgatct ttgtaaaaat atttaccgag
   144541 gcgttctaat tccgacttga taaaggcata atcaaaatgc acatggtgtg cgacaaaata
   144601 atgcccttcc atcatcgcct cgactttatg ggcgatatcc gcaaataccg gtgcacttga
   144661 aacgtcacga tcggttaatc cggtgagttt ggtaataaac ggtgggatcg gaatgggcgg
   144721 tttaactaaa gaatggaacg tatccgtaat tttgaaatca cgtacattga caatgccgat
   144781 ctcaatcaca tagccgatgg agcttttatc accggttgtt tcgacatcaa cgacggaaaa
   144841 acctgccgaa agatcaaata atgaagacgg cataacaaag ggaactcagg cgaacgtgtg
   144901 ttaaaacgtt caggttattg gattttttga cgagagcgaa ctatatatcg tgttgaataa
   144961 acagattcgg ccgtttcttt gggaaaacgc aacttaagat caatggaacc gttatccaat
   145021 tcataacaat ctccgtcgca atacgattcg cggtcatttt cgatatcatc ggcgtccacg
   145081 gcaagtacgg tatcaccatc gttttggata tatcgagcta cgacagaata gtattcttct
   145141 gttggcagtc ggtagctaaa cgtatttacg gtaactatcg tatccagcaa aagatcgtta
   145201 ttatcaaaat cacctcgaaa gatacgaatc ggaacggaat tgttttttcc gttaatggtc
   145261 agattaatat tgaggtagcc atgcgaatat ttagtttcat ggcaatccaa aggcttaggg
   145321 tcgcaatcac tgtcatcgtt ttcgtcatcc atcaattcat cacatccgct gagtaaccaa
   145381 ataccactca ataaaacgga aaaaataaaa ataaaacgca gtgaaaaaac ttttcggtta
   145441 accggcatta gaattgtcct cccaaagctg catagaaacg aagaccgggt attgtgcgca
   145501 tattcatgta ttgtaaaccc gatcggataa aaacaaatct ggaaaattta tgagaaaaac
   145561 cggcttgaac cacgggtgtc caaccttctt ttttttctaa tttatgtccg tcaataatac
   145621 cagagcttgt accccgataa tcaataaaag tcacgcctat accggcacca ataaaaaggt
   145681 caagcttatt attaatccag aattttttat ccaaaccgcc ggcaataacg tagcgatatt
   145741 ctttggcttg aaaataaaaa cgaggcccgg cctttactaa gacagctttt ccgtaagggc
   145801 gaccataaaa cgaaccgaaa acgcctaaaa attgattacc gttgatatta tgacggtagg
   145861 ttccgcccaa agcaagatcg ccgtagtttt taccgccaaa accaggtaaa cggaaaggca
   145921 tttcaaaata cgccgaatgt ttggcgcggt tttcgtatga catcgctccg ccacgatcaa
   145981 actgtgcgcg gaggccggat ggatagccca aacaaaacaa aaaagagaat agaataattt
   146041 ttttcatcat caagcaattg ttatttcttt ggtcgtatat acatcttgga tagcattaag
   146101 taaacgtaca ccatctccca ttggtttttg gaatgctttg cgaccggaaa taagacccat
   146161 accgccggcc cgtttattga taacagctgt ttttacagct tccgccaaat cactggcgcc
   146221 tttggattcg ccacccgaat tgataagtcc tgcacggccc atataacaat tggccacttg
   146281 ataacgcgtt aaatcaatag cgttttccgt tgtaagttca tcataaactt ttttgtgcgt
   146341 tttgccgaat tttaacgctt cataaccgcc gttattggtc ggaagttttt gtttgataat
   146401 atcggcttgg attgtaacac caagatgatt ggcttgtcct gtaagatcag cggaagtatg
   146461 aaaatctttt tcagaagttt tgaatgcgtt gttacgcgta tagcaccaca aaatagtagc
   146521 cattccaagt tcatgtgcgt acgcaaaagc ttcgctgact tcctgtattt ggcgtgcgga
   146581 ttcggcagaa ccaaaataaa tcgtggcacc gacagcgatc gcacccatgt cccaggcctg
   146641 cttgacggaa ccgaacatga tctgatcgta tttgttggga taagataaaa attcgttatg
   146701 attgattttt acgataaaag ggattttatg cgcatacttg cgtgcgcaag cgcccagtac
   146761 accataggtt gaagcgacgg cgttgcaacc accttctacg gcgagtttga cgatattttc
   146821 aggatcaaaa taaatcgggt tgggagcaaa agaagcgccg gctgagtgct caatgccttg
   146881 gtcaacagga aggatggata aataacccgt tccggctaaa cgtcctgtgc caaataacga
   146941 ctgtaaactg cgcaatacct gaggatggcg gtcgctatgg ataaaaatac gatctacaaa
   147001 atccggaccg ggaatgtgaa tctgatcttt tttgatgcct ttacattcat atttcaaaag
   147061 tgcatccgcc tctgcgccta agagttcctg aatggtttta agcataaatc tctcccgata
   147121 ttttaaattg aacaaatatt attcttatta aaaacgaaga aacttaacca aatttataag
   147181 cccattcaat gagaattttg tggcgaagta gtaaaaaact gccttatatt gccgccctga
   147241 ctaactcagc tattgtgaaa aatgaaacta aacgacgata ctcaactcac tttggactac
   147301 gaaaaaaaac cgacccgatc tgaatcggat ctatttaagg aaacgatcat cgttcgcggt
   147361 gcgcgggttc ataatcttaa aaatgtcgat ttggagattc cgcgtaacaa acttacggtt
   147421 attaccggtt tatcgggttc cggtaaatcc tcgttagcgt ttgatacgat ttttgccgaa
   147481 ggccagcgtc gatatgtcga atctctttcc gcgtatgccc gtcaatttct caatatgatg
   147541 gaaaaaccgg atgtggatct cgtagatggt ctgagcccgg cgatcagcat cgagcaaaaa
   147601 acagctagcc gcaatccacg ttctaccgtg ggtacggtta cggaaatcta tgattatttg
   147661 cgattgttgt atgccaaagt tggcgtgccg tattgttata attgcggacg tgaagtaaag
   147721 aaacaaacgc gcgatcaaat catcgaagca attctcaaaa tgaaaaaagg tgcacggatt
   147781 cagattttag caccggtcgt gcgaggccgt aaaggtcact atcaggaact gtttgagaaa
   147841 ttacgcaaaa gcggattcgt acgcgttcga atagacggag aaatccgcga attggaagaa
   147901 gtagataaag tttcacgtta tcagatacac aacatcgaag tcgtgataga tcggcttgag
   147961 ttaccggaaa aagcggatca acgtcttata gattctgtgg aacaggcgct cgaaatcggt
   148021 gaaggtgtat tgatagttgt ttccggtgaa ggcaaaaaaa atagcgatgt gacttacagt
   148081 caacatctag cgtgtctgca ctgtgcgatc agttatgatg agccttttcc caaggctttt
   148141 tctttcaata gcccgtttgg cgcttgtccg gcgtgtgaag gtctcggcgt aattcgtgaa
   148201 atcgatgaaa ccctcattat acccgataga gcaaaaacag tcaatgaagg tgccatcgct
   148261 ccgtatgata atccgaaagc cgatacgtgg ttttaccttc agatgaaaaa aatattggaa
   148321 atgaacggtg ctgattttga tacgccgatt cataagctgc caaaaaaagc gtacgaaatg
   148381 ttgatggaag gtaccggaaa aacgaaacat gcgttttatt attattcagg caataaaaag
   148441 aaaaactaca acggaacgta caaaggcgta aaaggtatta ttcgtcactt ttttagcgat
   148501 acctcgtcgg agcaaattcg cgaatgggct gaaggtttta tgagtgaaaa agtatgtcct
   148561 tcatgcctcg gtggccgtct caaaaaagaa gcgctttcgt atcgcatcgg cgataaaaat
   148621 attcatgacg ccgttcatat gtcggttcag cgatgcgctg atttttttga taaataccgt
   148681 attgatccgc gcatacaaaa gatcggggaa ccgatactca aagagatcaa tgaacggctt
   148741 ggttttttgc tcaaagtcgg tttggattat ctgacgcttg atcgcagtgc gcgtacactt
   148801 tccggcggtg aatcgcagcg tattcgtttg gctacacaag tcggttcaca acttgttggt
   148861 gttttatata ttctcgacga acccagtatc ggtttacatc aacgcgataa tgaaaaactg
   148921 atacaagctt tgacagattt acgcgatctt ggtaatacgg ttatcgtcgt cgagcatgat
   148981 cgtgatacga tggagcacgc cgattttttg gttgatctcg gaccgggggc aggtcggctt
   149041 ggcggaaaaa tcgttgcggc cggtgcgccg ggtgatttcg cacgttttga ttccgttacg
   149101 gcaaaatata ttcgcaatga agcgcgtata gaagtgccat cggtgcgacg cgatggcaac
   149161 ggcaaagttc tctcattgaa aaacgcgagc ggaaataatc tgaaaaatgt agatctgcat
   149221 attccgcttg gaaaacttgt gtgtgtgacc ggtgttagcg gatcgggcaa aagttcgctt
   149281 atcaacgaaa ctttttatcg tgtgctggca cgtcattttt ataaagctaa agatgcaccg
   149341 ctgccataca aatctatcaa aggtttagat catattgaca aagtcataga tattgatcag
   149401 tcgccgatcg ggcgaacacc gcgcagcaat ccggcaacat ataccaatct ttttagttta
   149461 atccgtgatt tgtatgccgg tttgccagaa gctaaagtgc gtggttacaa agtaggacgt
   149521 tttagtttta atgtgacagg cggacggtgc gaagagtgtc agggcgccgg attgaaaaaa
   149581 atcgaaatga attttttgcc cgatgtatat gtaccctgcg aaacatgtga aggcaaacga
   149641 tacaatcgcg aaactttgga aatccgctat aaagggaaaa gcatatccga agttttggaa
   149701 atgacggttg cagaagcgct ggattttttt gatgctgtac cacccatcaa gcgtaagata
   149761 caaacactgc acgacgtcgg attggattat attcatctcg gtcagcaagc cacgacactt
   149821 tccggcggag aagcgcagcg ggttaagttg gcagaagagc tctccaaatc cagcaccgga
   149881 aaaacacttt atattcttga tgaaccgacg acggggcttc attttgaaga tatcaaaatg
   149941 ttgatgaatg tgctcggcca gcttgtcgat aaaggcaata ccgtggtgat catcgagcat
   150001 aacctcgatg tgatcaaatg tgcggattgg gtgatcgata taggaccgga aggcggcgac
   150061 ggcggaggta atatcgtcgg cgaaggaaca ccggaggaaa ttgccaaaat caaagattct
   150121 tataccggca agtttttgaa gcaggaattg cggagattta ggaattaaaa attgattggt
   150181 tggtatataa tgtttagatt cgcccgtact tagaaaaaag tatattttaa taaagcataa
   150241 gtcggaatac gtccttggaa gatattcttt accgaatcga aaaagacaat acgcgcgtcg
   150301 ttgaaaaaat ggcggttcgg cgtttgtacg aagatttgaa agaaaaaatt catctggagc
   150361 ttatcgtttc cgaaggtatg gatattcgat attttgccca gcgcgatttg caccgaccgg
   150421 gattagcgtt agcggggttt gttaaacttt ttacgcatga caaaattcaa atcttaggca
   150481 acacggaaaa ttattacctc ctgagccagg acgacagcga acggcatgtc gcgcttgaaa
   150541 cgatttttga atttgaaatg ccgtgtatca ttgttactaa cggcaacaaa atcatgccgg
   150601 aaatgagcgc tctctgtgag aagaaaaaag tgccgctcat ggtgacagag ctttcgacga
   150661 cggatacgat ccatctgatc agcaactatc tcgacgagat gtttgctccg gaaatgacca
   150721 tgcatggatc tatggtggat gtttacggca tcggtatgat tctcatgggc aaaagcggta
   150781 tcggcaaaag cgaaatagcg ctagatctcg tcgaacgcgg ccaccgtctt gttgcggatg
   150841 atacgattcg catcagtcgt acttcgggcg gtattctgat ggctcgcgga catgatacgc
   150901 tcaagcattt tcttgaagtg cgcggtgtcg gcattcttga tgtgcgtgaa atgttcggta
   150961 tccgtgccgt gcgtgtacaa aaacgtgtcg aggtcgtggt tgatttacaa ctttgggacg
   151021 gcaaagaaga atacgaacgg cttggcttgg atgatcatac cgtcgaatat ctcggcgtaa
   151081 aaataccgat cgtcaaactg ccgatttatc cgggaaaaaa tattacggtt attgcggagg
   151141 ccattgcact caacctgctt ctcaaagttt acggttacga tgcggctaaa atttttacag
   151201 atcggttgtc tgaagaaata agaaataaaa ataaaaacgc taagtcctac gttaccaata
   151261 aatttttaga tggcgataaa gaataaaaaa agagggaaag tttgaacgaa aataaagtca
   151321 tggagatcaa ggtcggtatt acgatactat tagcgaccgt tcttttggtt atgagcatag
   151381 tgtggctcaa agggattact tttaagccaa acacgtttga agtcgaaata actttcaata
   151441 atacagccgg attgcagatc ggtgatcccg ttacagtcag cggtatgaaa gtgggaaaag
   151501 taacggatat taatctggaa gccgattccg taattgtgtc ggtcagttta tccaacagcg
   151561 tcattctcaa agaagatgta cgtgccgtta ttaccagcac agattttttt ggcggaaaaa
   151621 agatcgagtt agtcaccggc gtagcggata atattttcaa atccaaacga tttcgcggta
   151681 cgcgtgaacc cgatatcacg gagctaacaa gccagctcaa agaaatcgct accgatgtca
   151741 aaggtaccct tcagcgcgtg gacagcgtat tgatcggtgt aaataacttt gtcggcgata
   151801 aaaaaatgat caattcgctg aaaaacgcgg tatataatct tgattcgact acatcgcgcg
   151861 tacgtcaaat cgttgagcgc agtgatgtgc gaattgattc gacgctttcg cggctctcat
   151921 cctctaccaa aatttttaaa accatgttga gtcgtacgga tgcacagatt gattcgacat
   151981 ttggcaatgt acgcctgatc acacaatccg ttatggatgt aactcaatca ttggacagta
   152041 ttgtaagtaa agtgcaaagc ggcgaaggca atctgggccg gatgatctat gatgatcagt
   152101 tatattggcg aatcaatcgc accacacaac aattagattc tttggtcggc tcactgcgcg
   152161 aaaaaggcgt gaaggtcaat gttaaattgt ttggcgacta agcgcagtac aagattatga
   152221 attttcttct ttccattatg ttaagcttgt ttgccggtgt attgatcatt gccggtggat
   152281 gggttttttc atccaaaaaa aattgggaat cccgctatct tgattttttt atcgctttgg
   152341 gtgcgggata tattctcggt gtagcgcttc tcgatatgat accggaagct tttcatgcga
   152401 cgccccatgc gatgtattac gtggtcttag gttatctgat cgttcactta tttgaacacg
   152461 tcttaacacc gcactttcat tttggcgaag aaacacacaa tcaccttatt tccaacgtgg
   152521 ttagtacatc ggcgctgatc ggacttatgg tacataattt ttttagcggt gtggcgatcg
   152581 gttcgggcat gttgaccagt gaaaaagtag gatggatgat atttgcaggc acggtgcttc
   152641 ataaacttcc cgaaggtttt acgattgctt cgatcatgtt tgcgtctcat caccgccgta
   152701 catacggcgt tgtaggtacg attttgctgg cgcttgcatc gctgctggga actgtaatga
   152761 tttatttatt tgaaatggag tctttgcccg tcaaagatgt tgctttggcc atgtcggcgg
   152821 gaacttttat ccacgtagcc accacagatc ttattccgcg tatcaatgat tccggttctc
   152881 gtcgtatgcc attagtagtt tttctaggtg tcggattatt tttgataagt tatatgttga
   152941 tgcatccgta agcatcttgc ttggttatcg gttctattcc tttcggattc tcgaaaatta
   153001 attatcatat tggatatttc gtcgtatcat ttcggagctt tggtatgtta aaaaaaatat
   153061 ttagccggga tacattacaa aaggttctca acttcattga gcgagtgggt aatctattac
   153121 ctcatccggc tacattgttt ggtctttttg cattgaccat cgtgatcgta tcggggattt
   153181 tcagcgcaat ggatatatcc gtacaacacc cgggcaccgg tgaggttatt cgtccggtca
   153241 gcctgttaac agttgaagga atgcatcgca ttttatctaa ccttgtaacc aattttacgg
   153301 gattcgtccc tttgggtacc gttcttgttg cgatgctggg tattggtatt gccgaaggca
   153361 gcggtttgat cggctctgta ttgcgtctct tagtcatttc agcgccaaaa cgattattga
   153421 ctttcgtgat tgtttttgcc ggcgtaatgt ccaatgtggc gggcgaagtc ggttatgttc
   153481 ttttgatacc attagccgca gtaatatttt tggctgtagg tcgccacccc attgcgggtt
   153541 tagcggcagc gtttgccggc gtgtcgggcg gttacagtgc gaatttatta ttaggcaccg
   153601 tggatcctct tctggcgggg cttactcagg aagcggcgcg tatcgtggat ccggcttatc
   153661 atgtaaatcc tgcttgtaac tactattttt tggttgcttc gacttttgtt ttgagcgctg
   153721 taggtacatg ggttaccgaa aaaatagtag aaccgcgttt gggtgtttat caaggtgaag
   153781 aaaaacctga agaaatcaaa ccgctcacag tagaagaaaa acgcggtttt cgttatgcaa
   153841 tgataaccgg tttagtacta acagcgatta tcctcatagg cttaattcct gaaaacggtt
   153901 atctgcgcga tctgaaaacc ggttctgttt tgaaatcgcc gttcatggca catatcgttt
   153961 catttatttt tatactcggt gcagcgctgg gaatcgctta tggttggggt accggaaagt
   154021 ttaaaaacga cagtgatatt atgaagagca tgggcaaatc catggaaaca ctaggcggct
   154081 atatggtgct tgtttttttt gcagcgcaat ttgtcgcata cttcaactgg acaaatcttg
   154141 gtctcatcat cgctgtaaaa ttagccggta ttctgaaaac ctccggatta ggaagcgttc
   154201 ccttgattgt ttcgatggtt tttatgacat catttgtaaa tttatttatg ggcagcgcat
   154261 cagccaaatg ggcgatcatg gcgccgatat ttgttccgat gtttatgata cttggttatg
   154321 cgccggagtt gacccaagcg gcttatcgtg taggcgacag ttgctccaat gtcatttcgc
   154381 cgatgatgtc ttattttgcg ctcatcatgg cgtttatgca acgctatacc aaagacgcag
   154441 gtttgggttc actcatcgcc acgatgattc cttatacgat tgcatttctt attttttgga
   154501 ccgtagcgct ggtaatctgg cttatatttg aaatccccct ggggccggat gcgcgattat
   154561 ttttgcaagt gtaataccat cagattttaa ataaaaaacc tgagttgatg aaactcaggt
   154621 ttttttatac tctctcaaat ttttactttt agttgtgcta aaataatatt tgtcttcagg
   154681 cggttatttt ttgatcaaac ttttgccggt accgtttgat ctgttccatt tgcaattttg
   154741 atttattcta ccgtcacgct cttagcgagg ttacgcggtt gatccacatt gcatccgcga
   154801 agaacagcca tatgataaga caaaagctgt aacggaatca cggacaaaat cggaatcaga
   154861 aaatccatcg ttttgggaat ataaatgata tggtccgctt tgctgcggat atcggtattg
   154921 ccttccgttg taatagcaat cactttgccg ccgcgtgcac gcacttcttc gatattactg
   154981 acaaccttat catagatggc atcttgtggc gcgataaaaa cgatcggcat ttcttcgtca
   155041 atgagtgcga tcggcccgtg tttcatttct gctgccggat aaccttcggc atgaatgtag
   155101 gaaatttcct ttagtttgag agcgccttcc aaagccaccg gaaaattata tccgcggcca
   155161 aggtacagga aattcctacg atccttatac aattcggcga tttcacggat ataatcgctg
   155221 gctttgagaa tttcttttac tttttccggc aacatatcca tttcttcgac gatttgttta
   155281 ccttcggcgg aggacatatt tcgcaaccga gccagaagta cggtgatcag cgcgatgacg
   155341 gtaagttggg aagtaaatgc tttggtcgaa gcgacgccaa tctcgggtcc ggcatgaata
   155401 taaacgccgg catcggtttc gcgtgcgatc gaacttccga ccacattaca aataccgaat
   155461 acggcagccc ctcgccgttt ggcttcgcgc atagcggcga gtgtatcggc ggtttcgcct
   155521 gattggctga tcacaaaaac aacggtacct tcttcgacga tgggattgcg atatctaaac
   155581 tccgaagcgt attctacttc gacattgatt cgcgcaaatt cctcgatcat atattcagcc
   155641 gtcaatgccg catgccaact ggtgccgcat gcgatgaata cgattttttt tgcttttttg
   155701 agtgtgtcaa tatgatcaac aagaccgcca agtcgcggca tgccttcctc ggcgatcagg
   155761 cgaccacgca tcgcatcacg gatcgtgttt ggctgttcga aaatttcttt aagcataaaa
   155821 tgctcatagc cggctttttc gatctgactg atatcaaaag tgatttcttc gacttgtttt
   155881 tcgataacct tattgtctaa agttttgact tcgatacctt gacgtgacaa tatggccatt
   155941 tcattgtctt ccagatatac gacttgtcgt gtgtgtgcga cgatcgcgga ggcatcagaa
   156001 gccacgagaa attcttcgtt gccataaccc aaaaccagcg ggctaccaca gcgggctacg
   156061 atgatttttt caggttcgtg gcggcacata aaaacgaaac catacgcacc tttgatgcag
   156121 tatatggctt ctcgtacagc ggtttcgaaa tccataccgg atttgtaaaa ttcctcgacg
   156181 agatggatta ctgtttcagt atcagtttcg gttttaaatg tatgaccttt tttcacaagt
   156241 tcgattttca gagacgcata gttttcgatg ataccgttat gtatgacagc gaaattgttg
   156301 tcttcatcaa aatgaggatg ggcgttcgtc tgatttggca cgccgtgcgt ggcccagcgt
   156361 gtgtgcccca tgccgacggt tgcatcaaaa ttaacaccga ccaaatcttt ttcgagttcg
   156421 taaagtttgc ccgcttgttt acgtatttgt aatgattgat tttcgacaaa acaaacgccg
   156481 gccgaatcat aaccgcgata ttccatccgt ttgaggccat tcatcacgat aggcatggct
   156541 tgacgtcttc cgatataacc tataatcccg cacataaatc ctccaaaatt tgagccccat
   156601 gatagtaaaa taggctcgtt ttagcaaggt ttttgggttt gaataataac tcgttttttt
   156661 cgttgtaaca atacgatcaa gtcaacaaaa ttcttgtcaa aactctttgt ataacatata
   156721 tttataaatc agtttgtgtt atgggttaaa acctaagagc cattcttgaa gctaagccaa
   156781 aattaatcat atgatcgaaa cccaaacaaa aaagaacgga aacgctgcat tgaacttgtt
   156841 tcaagaacaa ggcgcgactg cacctattgt acgtgataag gcgcattacg atcatatttt
   156901 tgaagaagct ctgaattctt accaatccta tattcgcata ccggataccg aaacactgcg
   156961 taaagcttac gaatacagtt atctttcaca cttggggcaa ttccgtaaat ccggtttacc
   157021 gtattttgat cacatcatcg aagtggttaa aattttgatc gaacaaaaca tggatacggt
   157081 gacgctggtc gcggcttttt tgcatgatgt catagaagat accggaaaaa cctacgatga
   157141 tattgctaaa gagttcggca atgatgtcgc cggcattgtt gacggattga ccaaaatcgc
   157201 aggttttgaa ttcggcagtt tggaaatcaa acaagccgaa aatttccgca aaatgcttct
   157261 ttcgatgatc aaagatatac gtgtgatcat catcaagttt gctgatcgta ttcacaatat
   157321 gcgcacgatc gactccctgc cgcgcaaaaa acaagaacgc attgcgacgg aaacgcgcga
   157381 cgtgtatgcc ccgctcgccc atcgttttgg tatggctcgt acgcgatggg aattggaaga
   157441 tctcgtgctc aaagtgctgg atccggagtc gtattgggag ttatcacgct tagtcagtga
   157501 aaaaagaacg gagcgcgaat tttatattca gtccgtggcc aaaccgctta tcgaaaagct
   157561 caaagataac ggagtcgaag cgcaaatcat cggccggccc aaacactttt tcagtattta
   157621 taataaaatg aaaaagcgaa acaagccgtt taacgaaatt tacgacttgc ttgctattcg
   157681 tattatggtt caggaaaaaa cggattgtta tcgcgctttg ggttttgttc acagtacata
   157741 cacgccggtt actgaacgat tcaaagatta tgttgccgtt cctaaaatca acggctacca
   157801 atctatccac acaaccgtga tcgggcccga cggtaagatg gtggagatac aaattcgcac
   157861 acacgagatg catcagatag ccgaagaagg tatagctgcg cactggctgt acaaagaagg
   157921 caaaaatcag atggatcaga ttgataaaca gatcggatgg attcggcagt taatcgaacg
   157981 ccagcgtgat cagtcggatc ccggtgaatt tctcgaagat ctcaaaattg atttatttca
   158041 ggacgaagtt tttgttttta cgcccaaagg ggatcttatt cctttaccca aaggctctac
   158101 gccgatagat tttgcttttg ccgtgcactc ggaggtaggt ttacattgta tcggagccaa
   158161 agtcaatggc aagatcgtac ctttgcacac ggaactgcgc agtggtgaac ttattgaaat
   158221 tctcacctct gaacatcaat cgccaagcac gcattggctg gaaatagtca aatcatccaa
   158281 agcgcgcaac cacatcaaac gttattttcg taacatcgag atcgaacaaa gtatcgtttt
   158341 gggtaaagat cttctcgagc aggaattgcg tttggtacaa ctgcgcaaac cgtttgcgga
   158401 ctacgtggat gatttaatca aaatggctac ggagctaaca tttgaaaatc tggatatgat
   158461 gctttcatcc atagggcgtg gcaatacctc agcccaaagt atcgctcaac gcttagcgca
   158521 aaaaaatata gctccggaaa aacctcagga aagcagttcg ttttttaaac gttttatcaa
   158581 acgcagttct tcggaatcca aaggcattgc catcagcggt gttgacaata tgatgattca
   158641 ttttgccaaa tgctgtaatc cgataccagg cgatcagatt atgggctatg tcacgcgcgg
   158701 gcgcggggta cacttgcacc gcaatgactg tcacaatata ctggatgccg tcaaaaaaga
   158761 acccgaacgc gcgttagcgg ccaaatggca agtagatgaa accaaaaact tcaacgttca
   158821 aatacgtgtc gtaggacgcg atcgtaaaaa ctttcttgtg gacgtggcgc aaaaaatatc
   158881 ctccacggac actaatatcg tcagcgcgga agtgcgcacg acgggaaacg aaaccgtaca
   158941 tctttttgta ttacaagttc ggaatatcgc gcatctgaat atgatactcg ataaaatacg
   159001 gcgcgtgccc aatgtcatca ccgcatcccg agcggatacg agcggcgtag ccaactgaat
   159061 aacggattgt caaaaggaat gaaaatacaa tttttaggaa cgggcacttc gcatggcgtg
   159121 ccgatgatcg gttgtacctg tgcggtatgt gcatcggaaa atccgtataa taaaagattg
   159181 cgcacgtctg taatcgtcga agtaaaagag aaaaaaatat taatagatac ttcgatagat
   159241 ttacgacaac aagcgctttt acaccgtatt catcatgtag atgccgtact ttttacgcat
   159301 catcatgtgg atcatatttt cgggcttgat gagacacgtt catttaaccg attacaaaag
   159361 aaaaggcttc cgtgttatgc cggagcatac acgatcgagc agatgaaaaa agttttttcc
   159421 tacatttttg aatatcctga tattccgggc ggaataccga tgattgattt tataccggta
   159481 gataaacctt tcgccatcga agacgtccaa atcataccca tcacggtgat gcacggaaag
   159541 ttacctattt acgcctatcg tatcggtgat tttatatatg caaccgattg ttcgggaatc
   159601 ccggaaacat catgggagta tttttacaac gctgaaattt tggttctgga ttgtttgcgc
   159661 tacaggacgc accccactca ttttaatgtt gagcaagcgg tcgaagcggc acgaaagatc
   159721 ggcgccaaaa aaacatattt tacacatatt tcacatgaat tggatcatga tatcgtgaat
   159781 gccacgttac cggaaaacat caaattagct tatgatggta tgattgttga gagtaattag
   159841 aaagagaagc atgcgcaaaa atttttttct tgttcccttt ttattggttc tgcaaagctg
   159901 tgcgtttttt aatacatttt ataacgcccg gaaaagttat aaactaggca aagaaagcat
   159961 tgaacgctct tcgcgcacat cacgggaagc gccctccaat accccggaaa tgaaattagg
   160021 tttttttgat tcggaaccgg ataacatacc gggcgatgcc aaaaccgcat ttgatatctc
   160081 tatagaaaaa accaataaag tcgtcgtgct tcatccgaca agcggatggg tggatgacgc
   160141 actcttactg ctcggcaaat cctattattt acgttccggt gaaggggatt atcttgaagc
   160201 taaaaaccgc ctagaagttt tgatgacgcg gtttcccgcg tccgaactga tatcggaagc
   160261 aaagctttgg tacggtaaaa cattattcaa acttcagcag tttcaggaag ctcaggaaat
   160321 tcttcgtccg atcgaagcgc tcaatacaac ggaagacata cgtgctgaag ccaacctgat
   160381 actgggcgat attgcacttc ttgatgagaa cctcaacgta gcgaaagcct attatgcaca
   160441 ggctcagaag ctcgccaaag acgagaatct acgcaaaacg acgctgtata aatcagccta
   160501 ctgtttttac aaagatcaaa agtattcaga ttgcgtagta tttctaaata aactcaatca
   160561 aaacaatcta tctctttccg aacgctttga aatacttgtt ttccgatcac gggcgctaaa
   160621 gatggctgga cagtataaaa cagccattga tcacttggac aaaatcttgg gtgatccaaa
   160681 atacaaaaca tactttgcaa gagctgagtt tgaaattgct gatattttac gtatgcaggg
   160741 tcgttcaagc gatgccgaaa aacagtttat tttcatagtc aatacttatc ccgcggctcc
   160801 ggtcggggac tgttattaca tgttggggtt gttgcacgat acgacgcaaa caagaccatc
   160861 ggccgatttt attcccgatc ataatcgcgc aatgaaattt tatacattgg tacgtaacaa
   160921 atatgcttcg tcgtactatg caccaacagc tgttgcccgg ttgacgtttt ttgagaaaat
   160981 gaacttgctc aaagacgtta tgtcggtcaa tcgtcgttta gtagagatta ttgacggccg
   161041 gataaaaacc ttggaatcag gcgatacagc tacttttgtt ccggatgaat ttttagtgca
   161101 attggtcaaa gtagatacaa gtgtgcaaac taacttgctt gaggacaaag acaaagccga
   161161 gacgctaagc gataaagtta agcaagatct gaaaacgatc aacatggtat caacggaatt
   161221 aacggaaaaa caaaatgaaa taaattcaga tattttttcg ctcgttgagt taacacacag
   161281 ggatagtttg ttaaatttta gaaaaaaagt taatgaacgg ttggccgagt accatatttt
   161341 attagccgaa tactatgcaa atgaactcca tgaatatgat tcttcattgg cttattatac
   161401 gaaggtgacc gaattattta aagagtcaac cagtgaagag attgcttatt atggtttggc
   161461 gcgaatccat caaatcaagg gatttccaag ttatctgtct ttgtacaaag aggcttacga
   161521 aaaatttccc aatggcgtca tggctaaaac gggggaaacc attctcggat tgaaggccgc
   161581 aaatgcagat cgttatgatg atgtttttga ggaagcgcaa atgctgatgg tatccgacac
   161641 gcaatgggtg aaagccgctg atttgtttca aaccattgca cgaagcgatt cgaccaaaca
   161701 taagtggccc tcggtttatg cgatgggttt attggaagaa aagaaaaaaa ataatccgca
   161761 aaaagctttt ctgtactata acacgttatt gtacggtgca ccatcatggc cgtctgccga
   161821 aaagctgcga cagaaagtga acgcctacaa aaaagcgtgg aatattcaaa acgattcggc
   161881 cggttatacg atcgacacga cattgatccg gtggcatcat gacataagcg tgacgcaaat
   161941 tgatcaaaaa gcagattccg tcagaacaca ccaagaaatt ggtaaaaaca gtatgccgat
   162001 cacgaccgaa tctgcgcgta gagataccaa ccgtttacaa atgaccgact tcgaactcga
   162061 agaatttgaa aaccttttaa aaaagaaatc cggagcggat tctacgaaag ttgggaaaaa
   162121 acttaaaccc gatgagtcgc taaagaacaa aaaacaacgt cggatcaaag aagacgagtc
   162181 ggatgcgaca ccgatcatca aagaggaatg atgcgttata ctgttttgct gttaattttt
   162241 atgctgttcc tatacgcgtg tgtacccaat ccacagtatg gtacggttgc gtcgcgcaca
   162301 cggggtgaaa ttccggggaa agaaataata aaaaaaggat atcaaaccgg cgtcgcgtca
   162361 tattatgcac ataaatttca cggcagaaag acagcgaatg gtgaaatttt tgatatgcat
   162421 aaattaactg cggcacatcg tacgctaccg tttaatacgt tagtcaaagt aaccaatcta
   162481 aaaaacaaca aaacgataca agttcgtatc aatgaccgcg gcccttttgc taaagaccgg
   162541 attattgatc tatcgctggc ggccgcgcaa aaaatagaca tgatcggtag cgggacagct
   162601 gaagtaaggc tcgatattat caaagaaggt ccgtaaatcc atcggaggaa atcatcatgt
   162661 taggacaagc gccgtttgcc gtcgcatcgc ttatgttgat caattttgcg ttgaccttga
   162721 ttttcttttt tcgatctgtg cgttactcgg aagccgggcg atcttcttca ctgcgcttca
   162781 cggttttctt tgtagttttt ttgtgggtgt tgttgcaagc tgttttgagt tacatcggct
   162841 tttataccca attttcggcg ttacctccgc gactgatact taccggggtt ggccctgcgg
   162901 ttattgcggt tttagttttt cttataatac cgtcattgcg aaatataatt aacggatttc
   162961 gattggaaga tttgattttg ttaagcgtgg tacgcatacc ggttgaaatc atgttacacc
   163021 agttgtttac agtcggattg gttccggaag atatgacata caccggatta aactgggata
   163081 tcttttccgg tattacagcg ccggcgatga tgtgggtcgc aaaaaaaaac ttcacttggt
   163141 cgcgtagtgt tttgatcgtt tggcatgtac tcactttggg attactgata aatatcgtgt
   163201 ccatagcgat tttatctgcg ccctttccgt ttcagcaaat caattttgat cagcccaata
   163261 tcgccgtgtt ttcatttcct tttgtttttc ttccgacgtt tattgttccg atggtattat
   163321 gggctacgtt gacggggtta gtgaagatat ataatgtgaa aaaatagatg atgaaatttt
   163381 taagcaatgc cttcattttt ttagttttta tagtttcctg taaaacgaaa tcagacaatt
   163441 tgttgcagac attaataccc acgacaacta tagaagcaaa atcagataac aagattgcac
   163501 aagctgcaaa aaagtatgta gaaaaccctt tttcatatga cataacaatg agttaccacg
   163561 ttacgacata caaaaacggt caaaacacaa agaccgccgt ttatcctatg ggagacatca
   163621 atccggaaat cggtgtatgt acagatgttg tgatacgaag cctgagaatt gctggttgtg
   163681 atttgcagga actcgttcat cgtgatgcat ctaagcattt tagcttctac ccttatttta
   163741 tatggggaca aggaaaaccc gatcctaaca tagatcatcg tcgcgtccca atgcttaatg
   163801 ctttttttaa aaggcatgct aaaacgctaa cgattgcaac cgatgataaa cacataaatg
   163861 aatggcaggc cggagatatc atcatctggg atttaacggg ttccggtaaa atggatcata
   163921 ttggtattgt ttctgacacg cgcacgaaag caggtaacat accattaatt attcataact
   163981 atccttcccc aggttatgtt acacaggaga acgtgttgaa tcgctgggtg attaaggcgc
   164041 attatcgata cgatgggtgt aattaaaatg cgactaacaa cactttatag gttactattt
   164101 ttaatgtttt cgctttcctg ccagcatccc ggggttttat ttgaccaacc gatgccggtt
   164161 caagcgagtg cgaatttcag ttttccggat acattacttg ggcggtggtc tgctgttttg
   164221 ccggatacca catacaatcc cggtcctcca atgcgtgttg tacagtcaac aacaaatctg
   164281 atcatttcat caaaactcat gtacacgcaa gcgcaaacaa tttccgaagg tccgattatt
   164341 tttttagata c
//
LOCUS       DAOQGF010000002       109310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_36_length_109310_cov_7.601977, whole genome shotgun sequence.
ACCESSION   DAOQGF010000002 DAOQGF010000000
VERSION     DAOQGF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 109310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 109310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36_length_109310_cov_7.601977"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            504..845
                     /locus_tag="PK937_00675"
     CDS             504..845
                     /locus_tag="PK937_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31118.1"
                     /translation="METSSLFTIIAAFLSQSPIVVVWIVGAVLAYRQKPVYPRKSLLI
                     IIAMLLFLGQLFINTILNIVFPMMWDTQQLLLSYNVKAMVTSMIMTAGWILIMMALFM
                     KEKSRDGFGQN"
     gene            complement(852..1631)
                     /gene="truA"
                     /locus_tag="PK937_00680"
     CDS             complement(852..1631)
                     /gene="truA"
                     /locus_tag="PK937_00680"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNH31119.1"
                     /translation="MPRYKLTLEYEGTRYSGWQVQKDTHTVQGTLMYAITEELRNKNF
                     DFQGAGRTDSGVHALHQVAHLDCTTVLDPYKFRLAVNDRLPHDIHIKQIEKAPGSFHA
                     RHDAVARSYLYQISRRRSAFGKRFVWWIKDELDLARMQEASRFFVGLKDFKNFAAKDG
                     EADSTKVNLEELRIESRGDLILIRVVGSHFLWKMVRQITGILAEVGRGNMKPGDIAGL
                     FRQTSPSLARLTAPPSGLFLERVYYKNDERLQTLEPFLAIR"
     gene            complement(1833..2837)
                     /locus_tag="PK937_00685"
     CDS             complement(1833..2837)
                     /locus_tag="PK937_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNH31120.1"
                     /translation="MKKKEFEDILNKLAQQLTYEAQKNVFSSSKVFENRVRDILKTLI
                     TDSKVEIDYNPHPYAFPDIAVGEYGIEVKFTEKDNWRSVANSVFETFRNKEVVFIYVV
                     FGKMGGLPEVKWQSYQECVIHVRTSHVPRFELEIDAKSSLFEQMKISYDDFSVLPIEK
                     KMRYIREYAKARLKEGERLWWIEEKPEQEHTLPIQVRLYTELSQSEKRKLRAEASLLC
                     PKIVAGSRVKNKYNDVAMYLLTYHGVLASQARDLFSAGSVAMASDSIRGGLYIHRALS
                     DIEDEMINAAKTMEDALFVEYWKESVPPNKRIKRWLELADRNATGWKPSEVLFLNKKL
                     "
     gene            complement(2839..3798)
                     /locus_tag="PK937_00690"
     CDS             complement(2839..3798)
                     /locus_tag="PK937_00690"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNH31121.1"
                     /translation="MQRRVSQSILKKKKKFSIVSLFSGCGGMDLGFIGGFSIFGNEYY
                     KNPFEIKWANELNPYACKSYSRNIDSNIHCGDIRDYIGKLPSQADVLIGGFPCQDISI
                     NGKGLGVNGKRSSLYTYMIEAIKLTNPKVFVAENVKGLLMKTRRESLNKILHDFNELG
                     YNISYQLYHSADYGVPQSRERVFIVGTAKDVKSFVPPKTVRQKENWLTSYDAIHDLEK
                     VNENEQINHIWSLAKKSGEQGGRRLEPSKPAFTIRAECHGNIQFHYSLPRRLSMREAA
                     RIQSFPDNFIFEAKLRETERQIGNAVPPVLAWHIAQSVKDCLL"
     gene            complement(3896..4207)
                     /locus_tag="PK937_00695"
     CDS             complement(3896..4207)
                     /locus_tag="PK937_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH31122.1"
                     /translation="MQNETFGDTIRKLREESGLPLRKVAAHLDIDPSTLGKIERNERN
                     ANKEIIEKLAELFSVNSKPLLVSFLSDKVAYEIMEEDYTKDILKIAEEKVKYYKKQRH
                     N"
     gene            complement(4617..5387)
                     /locus_tag="PK937_00700"
     CDS             complement(4617..5387)
                     /locus_tag="PK937_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNH31123.1"
                     /translation="MMLMQGKQGLICGVANKRSIAHAIAKSLAREGAKLAFTYQGERL
                     KRNVEEIATELGSNLLYDCDVTKDDDIKRLFEAVSKDMPHIDFLVHSIAYAQKDDLEK
                     PFSTTSREGFRVAHDISAYSLIGMAREAMPLMKEKGGAIVALTYYGAEKVIPGYNVMG
                     VAKASLEASVRYLAADLGPSNIRVNAISAGPINTLAARGISGFTKILDITPQKAPLRR
                     NVEVDEVGDASLFMVSPLSRGITGEIMYVDCGYNITGM"
     gene            complement(5510..6964)
                     /locus_tag="PK937_00705"
     CDS             complement(5510..6964)
                     /locus_tag="PK937_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HNH31124.1"
                     /translation="MPIEIPEALKEYLKLFPGEYADAFSQGLDFERRTALRVNTLKFN
                     KTDFIHRLAQQNFRFTEIPSMPDALLVEGEDARISKTIEHFLGLFYIQSIASMLPPIV
                     LNPTASDFVLDVSAAPGSKTTQMAQMMKNGGCMVANDRDDKRIKTLSHNLDRLGVINT
                     AMVNMQGERIGNLVPGSFTKVLVDAPCSALGVIHKAPSAVSNLRHLNKFAFIQEQLVV
                     SGLKALRSGGTLVYSTCTISAEENEGLVDTILKRYPSLHLEAFDLPEGIAALPGITAL
                     GEKIFDPRVALTRRVMPSAINPEGFFLAKFRLDEAIEVRTGKTAQAEHKKMYALDGSS
                     HAEIRGMGKYFSGLFGFDENFWDQFVYHIKDDETFIASEDWRGRESVLNALFTHRVGM
                     RLARTRKGDMWKLSTNAAQLFSRDISRNIIELTDTREIETFVEAGTIRREFPGMAGGV
                     VVRSQGTALGCGVMHQGALKSQVPKSRTVISVDF"
     gene            7550..10069
                     /gene="priA"
                     /locus_tag="PK937_00710"
     CDS             7550..10069
                     /gene="priA"
                     /locus_tag="PK937_00710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNH31125.1"
                     /translation="MFAEVVFDLAIETRFTYGIPDAWESVIVPGSRVLAPFGRKKITG
                     YVVALSRHSERDQISPIHEALDPLPAFSEDLLKLAEWISDYYMAPLGQVLGAMLPPGM
                     ERHGEKLYVLKAVITDFEIKSLSKTKPVQSKILKALYVYKKLTLRQLVKKVGARNLIK
                     NLDELLRSAVVEERDVISDQLANIRTDTWVRLSSLLLGDEERFVWATDELQKRAPKQA
                     DILFYLDEATRQSEDEEPFALRQAELLKITEAPYSALIALEKKNWIERIEREVIRDPY
                     QRDYPRPNDVTLNDHQAQAVAAIAETLRNNKYYTWLLYGVTGSGKTQVYIESIRQTLA
                     LGKNAIVLVPEIALTPQAVERFKAHFGDQVAVLHSRMTMGERFDSWRKLQSGHFRIAI
                     GARSAVFAPVQNLGLIVVDEEHEHTYKQQDNIPPYHARDAAIMRASFCKATVILGSAT
                     PSIESYYNATTQKYGLLKLPIRINDVPMPEVEVVNMREEYEKHTSDWQPILSRALRQR
                     MKEELQHRRQAILFQNRRGYSSSVECFDCGHVAECPRCSIALTYHITGHKLRCHYCGF
                     HQPMYSECPKCDSLSIAQQGIGTQKVEEQLQMAFPNAGVLRMDVDSTSAKGAHHRILE
                     EFKNKNALILLGTQMVTKGLDFENVTVVGVISADTSLLLPDFRAGERTFQLLTQVAGR
                     AGRKNKIGHVIIQTMHPDRAAIRHARQHDFTSFFNEEISIRHDLLYPPFGRLMLIQIR
                     SEDEQKAVELSSVFSDILAGEIKRYPDLASGVAQLGPTPAPIVKIRNYFRYHILLKVD
                     KEADRSGSRIRSILRHTRKQVISRIPSGAEIRIDADPLSLL"
     gene            10082..11404
                     /locus_tag="PK937_00715"
     CDS             10082..11404
                     /locus_tag="PK937_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31126.1"
                     /translation="MSKRFEEKREAFEILNKELSDLIPLEQRVQKKDKSPHVVDPHAH
                     LPNDPAKSDATGAVPKEWLDEQAALHEKYKLVERVYKFYRNIIQNMSSSIICMDMAGH
                     ITFLNANAAKTLEYQVEELLGHPMTDIVAEPGRESKIIDRMLIGNKRFESKEVGLIAK
                     SGAVLPIGFSTAPLIENHEQIGVIITFRDLTEMNLMRKQVERMDRLATLGELATGIAH
                     EVRNPLGGIKTAAQVLEESFEDNDRRLELVSRIVREIDRVNHLLTDFFKFAKPVKPTR
                     DHFDVESLIDGVYLLLAAQMNSKKIRFKEDFSSTLPKVYADPHQTEQVLMNIILNAVQ
                     AMPQGGDIIVRTYTTTVGESKSMIHHTDEFFDKNIPFVAIEISDTGVGIPQENIEKIF
                     NPFYTTKPDGMGLGLSICNRLLTENNGNLHVESAVGKGTKFVIMLPTR"
     gene            complement(11385..11756)
                     /locus_tag="PK937_00720"
     CDS             complement(11385..11756)
                     /locus_tag="PK937_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNH31127.1"
                     /translation="MNPLTLKQHALWLRAVEEFNRKQFYICHETLEALWRDTNEETLK
                     RLLQGVLHIAVALYHLDRNNAVGYELQMKKGLVKLNAGLRVPSDLPIRNDLIVFYREV
                     ADFNRMRKSHIPMLRFNASAA"
     gene            complement(11753..12199)
                     /locus_tag="PK937_00725"
     CDS             complement(11753..12199)
                     /locus_tag="PK937_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH31128.1"
                     /translation="MKTVSAIISGREPYSVTLGTNVFDVIQLMASKNIGAICIVDSSA
                     TMRLRGVFSERDLLKRVVLQGLDPRALTIETVMTKNVAVASAGESYDVCMERMKRVNS
                     RHLPVVEGEKFLGMISVRDLLETNLEEKDAELKMMNAYIHDMPATV"
     gene            complement(12304..12744)
                     /locus_tag="PK937_00730"
     CDS             complement(12304..12744)
                     /locus_tag="PK937_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31129.1"
                     /translation="MSEISPPNTKLPILRIIWGAVIFSLFVFVAVVHLVILPTLGEKE
                     NESLSSLPFPVHYVFMFIAAGAAVAFVFLRQKRDITVAELSHDPKAAEQERIKRSILL
                     FAVAEMIGLLGCSILAFGAPYAQGMGLWGISFILLAMEFPRTED"
     gene            complement(13158..14090)
                     /locus_tag="PK937_00735"
     CDS             complement(13158..14090)
                     /locus_tag="PK937_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31130.1"
                     /translation="MGRKISPSDLLANSFLRMAKQFNANVELIKVLKSRTYKIGDANV
                     LVRASSDGNKRYFFGINYITVEEIANLENPFIAFICGSIERIVIIPAKLLFNHLHQIS
                     HDRNGEYKINIDQDLNIVLSGRSNRLECKTFINNWDLLLSPPKFDENEKPKTVEESLH
                     SVLQGRLLEIGNIRGYQTFCPDKSRKFNDKNLEEVSTLKTCPELQFSDYDLLRQIDVI
                     WFKTRGNNYIPEYAFEVELSTGVWSGVGRMATLMDYSNVGLYVIANDSKKYSQVINSF
                     TEYQDRYKFIANDLVGELYSAELNLKQLRIDIGL"
     gene            complement(14083..14283)
                     /locus_tag="PK937_00740"
     CDS             complement(14083..14283)
                     /locus_tag="PK937_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31131.1"
                     /translation="MEADNEVLFRITKEDLREEALHYLGREMNEEEYTKAKKLLEFGA
                     GENLRYIYYGVFLEIMDKSNNG"
     gene            complement(14316..14849)
                     /locus_tag="PK937_00745"
                     /pseudo
     CDS             complement(14316..14849)
                     /locus_tag="PK937_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(14870..15349)
                     /gene="smpB"
                     /locus_tag="PK937_00750"
     CDS             complement(14870..15349)
                     /gene="smpB"
                     /locus_tag="PK937_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNH31132.1"
                     /translation="MATATEKKKYEYKSIATNRKARFEFEIIDTLEAGIALTGSEVKV
                     LREGKINIQDCHARIENGEVILYHMHIPEYKQANYNNHEEYRPRKLLLHRVQIRKLER
                     QVQEKGMTLVPLSVYFNERNRIKVQLAVARGKKLYDKREAIAKKDQKRTEQREYKVR"
     gene            complement(15353..16294)
                     /locus_tag="PK937_00755"
     CDS             complement(15353..16294)
                     /locus_tag="PK937_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH31133.1"
                     /translation="MTDPNYSISVVIPLFNEEESLPELTEKIHTVASRMGVVYEIIFV
                     DDGSTDNSYTVIQKLKQQYAAVKALRFRKNFGKSTALSEGFKMATGTYVITMDADLQD
                     DPEEIPHLVNKLQEGNDLVSGWKKVRHDPISKTIPSRFFNYVTRVMSGIRLHDFNCGL
                     KAYRREVVKDVNLYGELHRYIPVLTKWEGYRRITEIVVHHHPRKYGQSKFGLSRFLKG
                     FLDLITVVFLTRYVKRPLHFFGFFGSLSLLAGLGICGYLSFDWFLGTPIGQRPILFLG
                     ILLIMIGFQIISTGLIGEMLVHNFNRQKEVPVKERIE"
     gene            complement(16307..17128)
                     /locus_tag="PK937_00760"
     CDS             complement(16307..17128)
                     /locus_tag="PK937_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNH31134.1"
                     /translation="MHYHTKYHRSFFLSFFGFMALILFSGCGAPKEVIRQPADITTVT
                     PLEVLRRCNENAAKIRHIKSVATMNVESPKSGGQFTAQIAIRLPDSVYIKIEGMLGID
                     GVKASLNRESFVVYNIINKQVIIGKTSASAIRRTFDYNVNYDELVELLVGLPRVPESE
                     LVRLREFSVDDTYFVMQFATGEGLRKIWFDPYAGFAVSRIVDYDSTGDVSVEREFSRF
                     QKIDGRYFPKYVRLVRPREQDLLSLFFDMRTVNESFNSSIFKIKYPRDIDIVEMQ"
     gene            complement(17112..18503)
                     /locus_tag="PK937_00765"
     CDS             complement(17112..18503)
                     /locus_tag="PK937_00765"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31135.1"
                     /translation="MRNRIYKASVFLLILPLLWSCGSRMHSQIGTQDSRYDAYKPAAV
                     QHLVDALIADMSGEFGEAEMHYRFALLSDSNALPVSNALAEDYLELGRFDEALALVQR
                     VLIKDPFNPDALEVLADIHIRQHNFDKAIQTLELLSRRYPSNIDAHYRLITIYEVQGK
                     AKEAAVHYARLLDVIGANTLLSLKLGEIYMRSRAYDKAVDVYHRARSSDPNNTEILSA
                     LAMAYRFNKEIPKAIEIYESLAELQEDNLQVHASVGLLSIQSGNYEKAVRAFRHADRL
                     QPNNFDFLRSLGFALVQLKRSVEALDVLERASMLNGRDLLTMNLLAPIYQNQKRFTQS
                     DSVFERILVFEPNNEVILNNYSYSLAERRIRLDKARQMIEKALKKAPDNGHYLDTMGW
                     VLFQLEEYAQALIYTQRAVAVHPNSEEVWTHLGEIHHKLGNTQDALSAFQKALTINPD
                     NSMLTEKINALSH"
     gene            complement(18548..18883)
                     /locus_tag="PK937_00770"
     CDS             complement(18548..18883)
                     /locus_tag="PK937_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31136.1"
                     /translation="MKKIQLKKIAHARSGDKGDSSNVGLIAYKQEYYSILCAQATPEA
                     VRRHFSKIANGPVDRYEVPNLLALNFIIHDSLGGGGTESLKTDAQGKTHGMGLLEMEI
                     EVPDDLIVA"
     gene            complement(18880..19137)
                     /gene="xseB"
                     /locus_tag="PK937_00775"
     CDS             complement(18880..19137)
                     /gene="xseB"
                     /locus_tag="PK937_00775"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNH31137.1"
                     /translation="MSKKKQNEESSETTFEASLKELETIVDALEHGDVPLDETLVKFE
                     RGMKLVEFCQAKLNTAEQKLKILIKDKDGNYTLEEEDEEAV"
     gene            19358..21196
                     /locus_tag="PK937_00780"
     CDS             19358..21196
                     /locus_tag="PK937_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31138.1"
                     /translation="MVLRIALWLCVAWPAMAQIKVVVFPFHNTTQAQKNDWLSHGFAE
                     ALNTELAKDTALDVIDRTDVLAEIKSKGWKQADIKDEAKAMAVARSLEANRMVYGVFD
                     AKDSSIKVLTKFFDLRTGVADGKNTYIGEGKFTTNNVHAMYGQIANKALVSFGRKTID
                     EIGGPTKGAINVDAYEPYIKGILKYDESSTVDDYNKAIEMLTQAATIDTGFALAYAGI
                     AKAYAKIARIQDVSGKLTEKEESYNKALEAGLRAVRIDKRLAVGWTALALTYRELKDR
                     DKLIEAARKAVQIRVSQYDAYDMLADAFSPSFFPQYKNIDSSIFYRKKSVTYNTKFAS
                     GFRGLGGDYMDKGDYKNAEQAFQKAVSINPKHAGSRDRLGQIYFAQGDYIKAKDNFSE
                     AIRLDAKTPFGYTHLADVLYMEGKYAEAIAAYDSALHHNPKFAMAHYGLAWTLLSSKQ
                     RSLRDSVRALTHATQAVEISGQKNAAFLSVLAEAQFANGNKEKAIETITLARALDSAN
                     TDYELSETRYAGKDKSGDYAYAMRRGQMFWKQGRGADGVKEFEEANKLNPKNVHVLLG
                     LARYYDQDKQYKKAFDHYFLARACDWDKKYVKQIQERMNALEKHSK"
     gene            complement(21254..21628)
                     /locus_tag="PK937_00785"
     CDS             complement(21254..21628)
                     /locus_tag="PK937_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNH31139.1"
                     /translation="MSHATDTGKHGENLAEIFLKQKGYDIRHRHFRFRNGELDIVAES
                     KTFIVIVEVKTTDLRRHDESAFGEPETWLTSKKQKFLKQAAEYYLAKYPAPEKDCRFD
                     LITVRLRSDGEEIRHIENAFWM"
     gene            complement(21785..23224)
                     /gene="dacB"
                     /locus_tag="PK937_00790"
     CDS             complement(21785..23224)
                     /gene="dacB"
                     /locus_tag="PK937_00790"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNH31140.1"
                     /translation="MMWRYGCILFFTAASLFSQYRSTQLDSVRRAIDAVYTDDFFKDI
                     RTGFFAKSLLTGQVIHQKNPDSLRSTASCMKLVTTAAALDRWGTNKSFRTEFRYDGAI
                     KNGVLNGDLYIRGLGDPYFLPEILLRAIYHLKAMGLTEIRGDIVGDDTYLIDTENAET
                     NDRAYSAVGGALGFNFNIINICVRPGEKTGDSAIVFIEPISSLVKVINGVKTTDSGEG
                     IGVNHNNTFIRYNGNQMTVALYGTIAKNENEFVIYKRVPNPTWWTIANIKETLGIMNI
                     KVSGGLRTGKTPKASKILIDPPSYDLSYIVSGINKWSNNYVVGQLLMMMGAEAFGEPG
                     TDEKGIRALQPFLRKVGISEKEIQMADGSGLDERNKMTTRAQVRLLEYMYRDFRYASD
                     YIASLSIGGIDGTEKKRFIKRKDESSLIGTTRLKVGFLYGVSGLSGYIETQSKDIIAF
                     SILTNGYPKEYYETMRQFEDRIVKALGGL"
     gene            complement(23231..23707)
                     /locus_tag="PK937_00795"
     CDS             complement(23231..23707)
                     /locus_tag="PK937_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNH31141.1"
                     /translation="MTFKKGPIQGVILKNHKKFVDDRGWLTELYRQDELASENVPVMG
                     YTSLTLPGVARGPHEHVQQTDLFCFIGPSNFKLYMWDNRKDSPTYGHKMVVFVGEDNL
                     QSVIIPPGVVHAYKNVGGKPGIVHNFANTLYAGKGKKEPVDEIRHEHDPQSPFQLD"
     gene            complement(23714..24070)
                     /locus_tag="PK937_00800"
     CDS             complement(23714..24070)
                     /locus_tag="PK937_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNH31142.1"
                     /translation="MKNETKETVVESTDPAEIKKKRNRRILFWSLIGIGALTIISGNY
                     GAYQIYKIKQQRAAVEKEIEKLKREHAVLADQRDRLKTDLEFIEKIAREKYTMIREGE
                     RVYQVLSKSETTNKKK"
     gene            complement(24082..25362)
                     /gene="eno"
                     /locus_tag="PK937_00805"
     CDS             complement(24082..25362)
                     /gene="eno"
                     /locus_tag="PK937_00805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNH31143.1"
                     /translation="MSHIHHVHAREVLDSRGNPTVEVEIHLESGAMGRAIVPSGASTG
                     EHEAVELRDGDKKRYLGKGVQKAVHNVNTIIAKKIMHMEAGDQFTLDHTMIALDGTAN
                     KGKLGANAILGVSLAAARAMANEVNIPLFQYIGGIQANTLPVPMMNIINGGAHADNNV
                     DFQEFMVMPVGARSFTEALRMGAEVFHHLKTVLKGKKYNTAVGDEGGFAPNLKSNDEA
                     IEVILQAIQAAGYKAGKDIMIALDPASSEFYQNGKYVFKKSDGRKLSSEQMAAYWENW
                     VRQFPIISIEDGMAENDWEGWKHLTDAVGKKIQLVGDDLFVTNPKFLAKGIEKGVANS
                     ILVKVNQIGSLTETIDAVQMAHKAGYTAVMSHRSGESEDTTIADIAVALNCGQIKTGS
                     ASRTDRIAKYNQLLRIDEALGGRAHFPGRKAFKV"
     gene            complement(25405..26379)
                     /locus_tag="PK937_00810"
     CDS             complement(25405..26379)
                     /locus_tag="PK937_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH31144.1"
                     /translation="MTLDDVFDPIEDELEIFKTKFSTQLTSKIKIVDTIARYVVSSRG
                     KNLRPVLVMLCSKMCGGSVTERSYRAAITIELVHTATLVHDDVVDGADMRRGLPAINA
                     IWKNKMAVLMGDYLLGNALISIVDIKSFDAMMLLSVATKRASEGELMQIDKSRKLDID
                     EPTYLRMISDKTGALIAAACELGAITTTEKPEDRKALNEYGELIGVAFQIKDDLFDFE
                     GEQTIIGKTKGRDLKEQKITLPLIYAFSRAPKSESKAVLKKIKKSVTSGDIKQIVAFT
                     EHYGGIEYAKKRLQEYSDRAIQTLNYFPDSPSRQALKKFVQYNIQRNK"
     gene            26888..29917
                     /locus_tag="PK937_00815"
     CDS             26888..29917
                     /locus_tag="PK937_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNH31145.1"
                     /translation="MPEQPSTQRRFEELWAGRGYSTRIQNFQELMRFKVRDILLISSL
                     YDSYILEEDGRLFELIQEEYRGLDMNQAPDIIHVSNGTEAIRMLDRQHRFDLILMTLH
                     IEDIHAIALAKKIRSLGHEMPIIVLAYDNIETQEVMTHRDADIFENIFIWQGDYRLLI
                     GIIKYIEDKMNFAHDSRAIGVQGIILVEDNVKFYSSYLPIIYTEIHRQSQRLISEGIN
                     LTHKFLRMRARPKILLCHTYEEAWKYYEEHNEYILGMISDVDFPRNGAVESKVGLLLA
                     RTIREKQSDIPILLQSTESSYSEEAREIGAAFVLKDSPVLLQELSDFMKQNFSFGDFV
                     FRLEDGSEVGRASDLSSLEETLHSVPAESVQYHGERNHFSNWLKARTEFWLAHKLKPK
                     KVTDYATVEDIRQHVIKVLKDYREIRQRANITDFSKDTFDPESSFARIGGGSLGGKAR
                     GLGFVKTLLNNYNMRYAFEGAKIFVPAAVAIGTNVFDQFLEHNNLRELALNSTDDREI
                     ADSFHSASYFPPEVITQLREFLEMVCVPLAVRSSSLLEDSQYHPFAGVYETYMIPNNH
                     ADTETRLQELLTTIKKVYASTFYKATKDYIKQTAYRLEEEKMAVIIMRTVGAAHDNRF
                     YPDFSGVAKSYNFYPMPPQTAKDGIASIALGLGKTIVDGGNTVRFCPKYPKHLIQQFS
                     TKEALKTSQTEFYAMKLGATEVDLYRDTKDRLIGKFTLDTAEKDGSLRFSGSTYSIEN
                     DAVYDGISRAGMRIVTFAPILKNKMFPLPEILDLLLELGKWGMGSEIEMEFAVNMSTP
                     PGKPREFGVLQMRPLVLRLENDALDVTPYRREEMICESTQVLGNGVIDSIRDILVVDY
                     DRFDRARSKEVALEIAQLNEKLVRDGKPYALIGVGRWGSLDHWLGIPVRWDQINGARA
                     IIESSFRDFDVDPSQGSHFFQNITSFQVGYFTVPHHAASSFVDWAWLAAQKAEEELNF
                     TRHLSFENPIVAVINGHDGKGVLLKPKPQPKESAVSL"
     gene            29914..32181
                     /locus_tag="PK937_00820"
     CDS             29914..32181
                     /locus_tag="PK937_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31146.1"
                     /translation="MNTQSLEFVQKTKQELNCKIIRTMLNYALEKAGPDAERKLIERM
                     GLTREYLQNENNWVSFDVYTHACEAMKTIFNDDRIMYRVGIYSFSNLENFGAIAKMAV
                     YLLKPESIYRRTAEVANQAVKFGTYRVVEIESNRLLLEFKNREGYKFHQHNCDYRLGV
                     FAGIPQLFGLPIARHRSIQCVSDGADRCLYEFRWLSRTWEIFGRHARIGLVMGILFAL
                     VLTVLNRFTFSHFSQPALLISGAAVLYLIGIILDMKHTAQYSAFINSGKSDEIEEAMA
                     FANQKYQEAADNITKLQAIIEGNAVLQSKLIKSELVEIVLEIIKHNLGYDRAMILFKN
                     NTQPLLDSPKLIGNLSPEMEQALLNYKINLTDDTAINTRVFYSGEPALITDMSEVKNS
                     TEMKAVQLSGTKSFVILPFKIGSRVVGTLTVDKISSPKALSREDVRILNTLLNMLSVA
                     LENAELYQDLEARVRERTAQLNETNKKLESAYDELKQAQAQLLHNEKMASLGKLVAGI
                     AHEMNNPAGILSGYIELMELHANRLRKDLEILDQFSAAHEDIAGTIQHIEKNILEITD
                     SIEPCKRSVWRIKSIIQDLRNFSRLDENELMPANINEGLENTLKFFKNQAEGRIRIIT
                     HFNPIPQVTCFPGHLNQVFSNVISNAIEAIDGTGTIEIHTSKSEPTSDVPTDHVVVKI
                     KDSGHGIPAEIQGKIFDPFFTTKDIGAGKGLGLSIAYGIIKQHSGNISAESEQDQGAC
                     ITIRIPIEYGYERKG"
     gene            32187..32522
                     /locus_tag="PK937_00825"
     CDS             32187..32522
                     /locus_tag="PK937_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH31147.1"
                     /translation="MSSDRVIEINTDSVAWESSDSKGVFYKSLRYDKETKAGAVLIRM
                     EPGSDYPEHEHTRGEDFIVLEGELIIGNKTFPAQSYVYSPPQSVHHPRTEKGCVLFAV
                     FHGKIINLK"
     gene            32529..33476
                     /locus_tag="PK937_00830"
     CDS             32529..33476
                     /locus_tag="PK937_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH31148.1"
                     /translation="MIAVHLALFFVSLVFGVNAVTSKYAMREIHPMALVFIRIVSAAA
                     ILYIIQRLWIKEKIQTRRDYFLLAGYSIVGIAINQVLFLKGLALTTAINATVLITTMP
                     AATIGIAILLRKEPPSFRKIIGSMISFSGVILLLGSERIDFSNDYFVGNLLVFLNGIS
                     YSFYLVISRDLLKRYHPSTVTTWIFVFGAVGVFPFGIGEISRLDWASVSLTAWACVAF
                     LIIFSSVIVYFINNWALRKTSSSVVAVYIYVQPIVAALISTQMMGETLRMETVIAALL
                     IFGGVFIVTLKMREEMKKEKSNANKEIDLVKESMIMKED"
     gene            33698..34306
                     /locus_tag="PK937_00835"
     CDS             33698..34306
                     /locus_tag="PK937_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31149.1"
                     /translation="MNKSNAKEQLDYIQTVLDRATVYTNISGWSAISVGLLGTIAGFY
                     SEWALKGSLDYQTYIASLVGCWSFCFIASIALAVFFSARRAKRMNEKIWGEPIKQAVR
                     HFSVFVFIGGVQTAACTFYHAYYLIPSVWMLCYGGGIIVGGLYSVKEIRILGLFFLAA
                     GSIALFSPVWNQIALMSTFGFGHIILGLIILKKYNQPLSHKS"
     gene            34321..34614
                     /locus_tag="PK937_00840"
     CDS             34321..34614
                     /locus_tag="PK937_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH31150.1"
                     /translation="MKTEIHHIDEVIHQKVRLGIMTILAARRDADFTELKQELTLSDG
                     NLSTHLSLLEKHHYITIQKQFSGRKPQTTIAITEIGKTALKNYLTILRKIIES"
     gene            34611..35036
                     /locus_tag="PK937_00845"
     CDS             34611..35036
                     /locus_tag="PK937_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNH31151.1"
                     /translation="MNHRISRISIFHKVPSRLQWLIAYIGFLSLVSISAYSYGLPRWL
                     DIIPFYDTIGHFVLMGILGILTLLVLRRTIRIKLDISVALAPIVISLISIADEYLQKL
                     SPHRTFSYLDMAANLIGIWTFYFIYRFYAKSKRVKEESL"
     gene            35033..36325
                     /locus_tag="PK937_00850"
     CDS             35033..36325
                     /locus_tag="PK937_00850"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HNH31152.1"
                     /translation="MTTPRLFAIMGIFFCTMIAWFILAGSISFRTDEKRLSFGPRMAS
                     HWGGVHKQETPQLFYHVKEWAVTKETSTNAAGASVTVEKSSEVDVKKNIECGKTQINA
                     DFEVDYRQKGLLWYSVYKVRFSGLYTFTNPSAKEEDVTIDFTFPAAQAIYDDFVFQWD
                     GQELLKTETIRESSDDPYRQDSKPGSLRANIKMKPGQIAVLKVGYTSQGTGTWEYSVG
                     EGTKRVTDFQLTAQTHFKEFDIPEFSISPGTKTETADGWSLVWQYKDIISDVRIGLGM
                     PKKLDPGPVAERISFFAPVSLLFFFCILIIIGAIRDVRMHPMNYFFLAAAFFSFHLLF
                     AYLVDHLALELSFAISSIVSVTLVAAYMRLVTSWPYTVRVVMPTQVIYLILFSYSFFF
                     EGYTGLTITIGAIVTLFILMQMTGKTDWNAIFKKDIKS"
     gene            complement(36399..37343)
                     /locus_tag="PK937_00855"
     CDS             complement(36399..37343)
                     /locus_tag="PK937_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31153.1"
                     /translation="MKSFLRLMLAGLILTVTTGRAMAGDDLEAKIKSMASANAQSYIK
                     PIVSGFGVAMNSGIFRSPETHSVLGFDFQLVTAMAFAPSSEKTYTPVVPNGYVPAASN
                     EANTAIGSKDPTTYYLGGIPNDQTNIVLPGGIDAPAGASGFFVAPQLTVGVPFKTDVI
                     LRFVPGIPAGKLGKVKLFGLGAKHNINQWLPISLPLDIAAGAFYQKMSIGDVFEAKAT
                     SFDILAGKSLNILIIKLGLYGGVGIESSSMDINYETTGTASGVPEGTKVKFSVDGKNS
                     MRGIVGANIRLLWLINVNPEMSFGKYRSTSIGVGLTLR"
     gene            complement(37577..38290)
                     /locus_tag="PK937_00860"
     CDS             complement(37577..38290)
                     /locus_tag="PK937_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH31154.1"
                     /translation="MKDVGRDTIKNRSSIIRSFFPGTGHSYDRVVHGFTLGLDYHWKR
                     MLLRHIPKDSRRVLDLACGTGIVTEGIAKRCPDADIVGVDITDDYLKIYERRIRSKNL
                     RASFLLGNAEDVKLDGIFDAVISSYIPKYVDPEILLSNINPHLKSSGVIAVHDFYYPP
                     NSVARAIWRKYNRAMNFVGMRFFPEWHEVFDGGLTSLIQSSNWFEKWQESLIRFEYEI
                     KTAHLLSFGSSAIIVARKK"
     gene            complement(38324..38809)
                     /gene="msrB"
                     /locus_tag="PK937_00865"
     CDS             complement(38324..38809)
                     /gene="msrB"
                     /locus_tag="PK937_00865"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNH31155.1"
                     /translation="MKKLLFAVWLMMTACSNAQHPSTHTVQNSTRKDQIVKSEEEWKK
                     ELSPEQYKVLRQQGTERAFTGKYWDHHEKGMYICAACGLELFDSQTKYDSGSGWPSFY
                     QPADKEVVSETHDTSYGMNRVEVHCARCGGHLGHVFEDGPDPTGLRYCINSLSLDFKK
                     K"
     gene            complement(38913..40193)
                     /locus_tag="PK937_00870"
     CDS             complement(38913..40193)
                     /locus_tag="PK937_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH31156.1"
                     /translation="MGRILIGLIILSMQLLHAQQDTLFIRAGRLYDGIGSRIQKNKLL
                     VVFNDRIVRVDDDNRPSYNGKIIDLREKTVLPGLIDAHTHIVLHPGDYDGQILRETPE
                     YRAVYGTVLARKTLEAGVTTIRDLGNEGAGLADVALRDAIQKKIVPGPRILTAIQPIV
                     PTGAYDLVGYSPYYEFPPIAFHADGADEIRTQIRRLSKLGADVIKIYMESYEKRETSK
                     DSLTGAMHYTPDELRVLVDEAHHNGLRVAAHVYSDTAARLAIAVGVNSIEHGLYLSDE
                     TCRRMAKKNIYYVPTLMVYELWRDGIIFSPVSDENKRKLARTVKQHTLSFQRALKAGV
                     KIVFGTDTFELPGTNAQELERMTAYGMNPTDVLRAATYASAMLLGVEKITGSIEDGKM
                     ADLIAVNGDPTENIAVMRNVVFVMKDGVIYKNEP"
     gene            40409..41071
                     /gene="rpe"
                     /locus_tag="PK937_00875"
     CDS             40409..41071
                     /gene="rpe"
                     /locus_tag="PK937_00875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNH31157.1"
                     /translation="MPIIAPSILSADFANLSRDIRIAEESGADWIHVDVMDGRFVPNI
                     TIGPMVVEHLRKITKLPLDVHLMIVEPEKYITAFRDAGADIISVHQEACPHLHRTLQQ
                     IRQSGAKAGVVLNPSTSHDTLQYVLDDVDLILVMSVNPGFGGQKFIPQAAHKIRALRN
                     MLGSRKVFIEVDGGINNETGLQVIQAGADVLVAGSYVFGAENPKSRIQSLKQLHASTT
                     GV"
     gene            41141..42157
                     /locus_tag="PK937_00880"
     CDS             41141..42157
                     /locus_tag="PK937_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNH31158.1"
                     /translation="MMPLPPFEFAAPATLDEAAKLLDQHKSHAKILAGGTDLIPSMKN
                     KLFEPQVLIGIGNIPEMKKIEYDPQKGLTLGSLVTITDIGHHPHITTHYPVLSDAARK
                     IATPLLQNKGTIGGNILLDTRCYYYNQSHFWRKAINNCLKKEGDVCRVAPSGHRCYAI
                     SSSDTVPVLYAMNASVTLVHAHGQRTVPLRAFYENDGMKFNKLEPGEILSQVHIPVLP
                     KGFRGAYMKLRRRQTIDYPMLGVMIGLQMDDKNICRNAQIVLGAVFSMPYEVKAAQDM
                     LIGQTITEDLIEKVADIAHQEAKPLDLTNFRPAYRKRMVRVFVKRILNVLTQKNTYVE
                     EILA"
     gene            42182..43450
                     /gene="purD"
                     /locus_tag="PK937_00885"
     CDS             42182..43450
                     /gene="purD"
                     /locus_tag="PK937_00885"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNH31159.1"
                     /translation="MNILVIGSGGREHALVWKIAQSPRVKKIYCANGNAGIRSLAECI
                     AIKPEDIHALADFAASHTIDLTVVGSETPLTLGIVDLFESRGLKIFGPSAKAAEIEGS
                     KVFSKQLMKKYGIPTAAFETFDNADQARSWIRSYDKPCVVKTDGLAAGKGAIVCMSTS
                     EALDAVDQIMSDKQFGSAGSQIVVEAFMEGEEASIFAVTDGTNYVVLPAAQDHKRALD
                     GDLGKNTGGMGAYAPAPVATHVVIERTKKEIIEPTLAAMAHEGRTYKGVLYCGIMVTP
                     DGPKVVEFNCRFGDPECQVLMMLISSDIVDLFQSVADNNLDPSAVTISDAHAACVVMA
                     SGGYPDAYAKGKIVNGLHTPLPENCAVFHAGTDWNENNIITNGGRVLGISATGADLKK
                     ALDTAYTVTREIHFENAFYRKDIGHRALKK"
     gene            43447..44997
                     /locus_tag="PK937_00890"
     CDS             43447..44997
                     /locus_tag="PK937_00890"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH31160.1"
                     /translation="MKPLYHIFFCTIIAASIGGVIALAQAPMKLSYGTANKEVIPVFT
                     GDQEYVSIKDVIALLDAGFFENTAKKKMVFTSNQHQFKVTGLNAFVVVDEKTTLQMPV
                     TTQYIDGAIFVPAVYFFSAINAYLSTPVVINGEKIIAKSAPAIKDTIKTSAVSISPNL
                     KDVIFEKKKNGLLMRIKTDGRFQSSDVEIWRNKNWLYVTVSGGYHSDLLAKDIKLSEQ
                     YKLIKEALIFQHKNSTQFSFQLNSDIAGYDISVDDKKDEIYVAWRIHQSSDLEFDNGG
                     SGTGAIDKDQWKINKIVIDAGHGGKDHGASGKGGTKEKDITLNIALKVGKIIAERLKI
                     PVEYTRDTDTYITLKGRTQFANQKNAKLFVSIHCNSNKSRKAHGFEIFFLSPSRNAEA
                     LAVARKENEVIDMEDDKHHYGDFTDEKFILANIMQSVFVKESEELASYVSKGIDKQVD
                     ITNRGVTQAPFYVLMGASMPSILVETAFISNPDEEKFLKSEAGQKKLSEGIVDGIKTF
                     IDIYERSN"
     gene            complement(45475..46620)
                     /locus_tag="PK937_00895"
     CDS             complement(45475..46620)
                     /locus_tag="PK937_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH31161.1"
                     /translation="MTKKLPLQKHLFDIPDEVTFLNHAFFAPQLRAMTEAGLQSVRAK
                     SSPWNYTPQHWFEDGETLRGLIAQIIGVASDDMAIIPSVSYGMAIAAKNITVRSDQNI
                     VVLHEEFPSNYYAWYYKAKDCGASLRVVHRTNQLNWTEAICENIDEKTAVVAVPNCHW
                     TDGARIDLERVSQKARSAEAALVIDASQSLGAFPIPMEKIRPDFLISVGYKWLLGPYS
                     LGYLYVDPKWQEGTPIEYSWLNRFGSENFSRLIEYTDKFRQGARRYDMGEFSNFINVP
                     MAIAGLRQIITWEVSAISESLLDLTSYAAQYAHSLGWVTISDSERIPHLLGIRHATSM
                     PSDIGQKLSENKIYVSIRGNAIRVAPYLYNQKSDMDRLFEVLKVYSR"
     gene            complement(46736..47572)
                     /locus_tag="PK937_00900"
     CDS             complement(46736..47572)
                     /locus_tag="PK937_00900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNH31162.1"
                     /translation="MKIHRIAVMALAVLMSCQPAKKEPAKAEATTVAAKNITGEEISY
                     AADNITMKGFIAYDAAQTGKRPGVIVVHEWWGHNEYARQRAKMLAEMGYVALAVDMYG
                     DGKKAEHPQDAGKFASETMKNFKGAKARFEKAVETLKNNPNVDASHIAAIGYCFGGGV
                     VLNMARQGIDMKAVVSFHGSLGAAEPAKEGVVKSRVLVCNGAADKFVSEKDIEGFKKE
                     MDAAKVNYVFKSYDGAVHGFTNPAVDSLGKKFNLPLAYNAKADADSWADMTALFKEVF
                     GK"
     gene            complement(47624..47980)
                     /locus_tag="PK937_00905"
     CDS             complement(47624..47980)
                     /locus_tag="PK937_00905"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HNH31163.1"
                     /translation="MYALLEEIENAIAAHELWKERLMAAIETGHSVWAVDKVSVDDQC
                     AFGKWIHMIEADYKGDHFYTTVRELHAHFHKIAGIVLNLALSGFKKQATEIYQGEYQL
                     ASQHLLFELHAWKKSL"
     gene            complement(47984..49123)
                     /gene="carA"
                     /locus_tag="PK937_00910"
     CDS             complement(47984..49123)
                     /gene="carA"
                     /locus_tag="PK937_00910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNH31164.1"
                     /translation="MNSNKSKALLVLEDGTFYEAWSFGADGEATGEIVFNTSMTGYQE
                     IVSDPSYYGQMIVMTYPLIGNYGISDEDFESRGPQAKALIVKEVCDYPSNWRSSHSLS
                     DLLKKNGIMGIEGLDTRAITRHIRNFGAMRAIISTTDTSVQRLLEKVKNAPRMEGANH
                     VDAVTCKTKYQWSEPNAEMWQFRENITGTHPYRIAVYDFGVKQNILRKFVERGCVVEV
                     FPSKTKADEILASNPDGIFLSNGPGDPEAVDYVIPEIKKFIGKKPVFGICLGQQLLGI
                     ALGAKTYKLKFGHRGANHPVKNLATGIIEITSQNHGFTVDPKTLNEKEIELTHFNLYD
                     GTLEGFRHRELPLFSVQYHPEASPGPHDSDYLFNQFMDRVKADKN"
     gene            complement(49125..49862)
                     /locus_tag="PK937_00915"
     CDS             complement(49125..49862)
                     /locus_tag="PK937_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH31165.1"
                     /translation="MIAPKHIMIMAHRGVTYSGFPENSLPGFQAVVDEGYDGVELDVR
                     LTRDKELVVFHDIRLDRLTTTGRGMVRTKTLEELKKLTLRSDRVQTGIPTLKEVLTVF
                     QGKPVFVNIEIKSEMPLRGKIEERVIDLIYNMHMRKQVVISSFNPLVVKKCQRIDPDM
                     RMGFIYEKRLPNLNHRLAKGLVVDSWHPNSVGVNALLMERAWEARCAVYPWTVNDEKE
                     MQRMIDLGVDGLITDYPNIAKKILQRI"
     gene            complement(49859..51016)
                     /gene="bshA"
                     /locus_tag="PK937_00920"
     CDS             complement(49859..51016)
                     /gene="bshA"
                     /locus_tag="PK937_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNH31166.1"
                     /translation="MKIGVTCYPTYGGSGVVATELGIELAKRGHDIHFITSDIPFRLN
                     TFRDRIYFHAVEVMNYPVFDHSPYSLSLAARMAEVAEFEDLDLLHVHYAIPHAPSAYL
                     AKQIIRKNNRIKDLKTVTTLHGTDITLLGMDRSFLNLIKFSIDESDGTTSVSKYLRNR
                     TYGEFKPNRKIEVIPNFVDTEKYRPHDKPEDSSHGFLSRITPNHEKLIMHISNFRPVK
                     RVGDVVKIFAKVRENINAKLLLIGDGPERRMCEQLVQDFGLDEHVVFLGKQEAVAELI
                     CCAHVFLLPSEEESFGLAALEAMAAGVPCVTSNAGGLSEVNIHNETGYLEKIGDVDAM
                     AADVVKILTDQDVYQRFRANARQRVVDNFGTDRVVQQYIDYYQKVIEGKNT"
     gene            complement(51026..51259)
                     /locus_tag="PK937_00925"
     CDS             complement(51026..51259)
                     /locus_tag="PK937_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31167.1"
                     /translation="MNEDTSQPDIQDEETSGEAASDTRRKRMWQIFGWSVIGALLLLI
                     VLNLFQWASPEINQYGLPVIVVLYVVYVFFRKR"
     gene            complement(51263..51733)
                     /gene="greA"
                     /locus_tag="PK937_00930"
     CDS             complement(51263..51733)
                     /gene="greA"
                     /locus_tag="PK937_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNH31168.1"
                     /translation="MSKFYVTHEGLLKLKEEIQHLKNVKRPEIANRVQVAREHGDLKE
                     NAEYHAAKEELQLLQSKINEMEMKVMNAVIVKEDEIPTDKVYILATVDLQNVKTKEKL
                     TYTLVPDNEADWENNKLSVNTPIAKGLLGKKVGDVAKVKIPAGELELKIIKISR"
     gene            complement(51755..52969)
                     /locus_tag="PK937_00935"
     CDS             complement(51755..52969)
                     /locus_tag="PK937_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNH31169.1"
                     /translation="MAQSTGNINERIERIQKDIARNGYDGWLLYSFRKNNPVASSILS
                     IPEHLTQKRRFFYYIPAQGTPKKLVHGIERYVLDTLPGEKVIFSSWQDLEAGLKAITA
                     GHKKIAMEYSPECNIPYVAIVDAGTIELVRKTTQAEIVSSADLVQYFDSTWDDQQLQL
                     HVEAGKALTDIIPQVWGFIREKITGRQKVTEYDVQQFIHNQFIERGLTTGDLPNCSVN
                     ENAGDPHYEPTKDHCKEIHEGDLVLIDWWAKKNHPRGVYADYTQMGFVGKTVPEKFAK
                     VFDVVVGARDAAIAFIKEEIAADRPLPGWKIDDVCRAHIVQHGYGEYFIHRTGHSIGE
                     EVHGNGANIDNLETKDNRRIIPRTCFSIEPGIYFVGDFGVRTEVNVYVGDKKDVLVTG
                     TPMQSSIFPILA"
     gene            complement(52980..53969)
                     /gene="thiL"
                     /locus_tag="PK937_00940"
     CDS             complement(52980..53969)
                     /gene="thiL"
                     /locus_tag="PK937_00940"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNH31170.1"
                     /translation="MKSKKTGDEFSLIRQLHAILESGYPTGKNVLCGIGDDAAWVRSS
                     AKQQVITTDAMVEGIHFDRRYMAFEDIGYKSISVNVSDIAAMGADPSFAVIALQMDPQ
                     TGTKNIHALYRGITKACRNYNVQIVGGNISRSSAFSITVTLIGNVAERNRPWLRSDAR
                     IGDVIAVTGDLGGSAAGLMFYDRLKKKPAYAWQRHCRPDARLEWAQILRRKNIRVHAA
                     IDISDGLSSDLAHVCRASRCGALIEAACIPVHAEIKKLSDVLKIDSMMWALHGGEDYE
                     LLLTMSPKEFVKAKKILKNNIHAIGTIVAGTSVVIADASGNRKTLEPAGFRHF"
     gene            complement(54154..54768)
                     /locus_tag="PK937_00945"
     CDS             complement(54154..54768)
                     /locus_tag="PK937_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31171.1"
                     /translation="MKYIVIFFVFVMTIVSCKPGPKPAPENAPAGNPHAGNPHANIDM
                     NAPGHVVFKAPEVWKSETPTNSMRKEQYRLPGSKPEDDAELVLSYFPGAGGSVDANLS
                     RWYGQFKQPDGGDTQSKAVIKNTENNKLKITVVRVSGNYMKPRVAGQMGGPVDETPAY
                     AMWAAIVEAPNGPWFLKAVGPQTTMDKYSKEFEQLIGTIDYILD"
     gene            complement(54847..55959)
                     /locus_tag="PK937_00950"
     CDS             complement(54847..55959)
                     /locus_tag="PK937_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31172.1"
                     /translation="MRTIRFILRKEFLQIFRNKGMLPIIFVMPFIQLLILSNAATFDV
                     KNVKFQIVDRDRSDLSRQLIERFTSTHHFILTDMPTHYAQAEESVLKNEASLIVEIPV
                     FFEKDVITGATVKLQLVINSEDGSTAGVIQSYAAQIIQDFTRQVVPFQQTQGMTQRIE
                     IIARNWYNPELNYKHYMIPGILVALVTMIGLFLSGMNVVREKEIGTIEQINVTPIKKH
                     QFIIGKLLPFLIIGLFELAMGLAIGRLVFDLPMLGGLPLVFGITIIYLILVLGLGLFI
                     STVTETQQQAMFIAWFFMVIFMLMGGLFTPLDSMPLWAQQTMWINPVALFIRILRQVL
                     LKGAPLHDIIPYVSALIVLAVTMFTLAVIRYRKTSE"
     gene            complement(55962..57062)
                     /locus_tag="PK937_00955"
     CDS             complement(55962..57062)
                     /locus_tag="PK937_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31173.1"
                     /translation="MKSFRAFIVKEFFHILRDKRTVLILFGMPVIQMILFGFAIRNEI
                     NDARIAILDHAHDARSQEVTQKILSSGYFQNVQTLTHDDQIAPAFETGDVKMVVVFEP
                     QFTARLYHEGVAHIQLITDATEPNMATTLTAYATSIIRDYSANLNPHAPTPIVTEVKM
                     LFNPEMRSVYLFVPGLIALLLMLISALMTSITITREKELGTMEILLVSPLRPIQIIVG
                     KVLPYLLLSLVNTSTVLLLARFVFEVPFRGSYALFYAEALLFIMTALSLGLLISTIAR
                     TQQVAMMASLAGLMLPVIILSGFIFPVSSMPVPLQIFSNIVPAKWFLIIVKGIMLKGV
                     GIEYLWKETLVLAGMTLLLMTISARKFKIRLE"
     gene            complement(57059..57814)
                     /locus_tag="PK937_00960"
     CDS             complement(57059..57814)
                     /locus_tag="PK937_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31174.1"
                     /translation="MKPNKSDAAITVRDLTKRFGSFIAVNAITFDVGRGEIFGFLGAN
                     GAGKTTAMRMIIGLLAPTSGTAQVAGYDVYTQTESIKKNIGYMSQRFSLYEDMTVQEN
                     ITFYGGIYGLSDAAIRTKSEMLLERLRMHDVARTMLRELPLGWKQKLAFSVALLHDPA
                     IVFLDEPTGGVDPITRRQFWDMIYEAAHRGVTVFVTTHYMDEAEYCDRVSIMVDGRMA
                     ALDTPRGLKQTFEAQSMNDVFIRLARPQQSKEL"
     gene            complement(57801..58721)
                     /locus_tag="PK937_00965"
     CDS             complement(57801..58721)
                     /locus_tag="PK937_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31175.1"
                     /translation="MISVEADHLTKRFGKTSAITNLSFQVKAGELFGFIGPDGAGKST
                     LFRILTTLMIPDEGRATVSGLDVVRDFRELRKRLGYMPGRFSLYQDLSVEENLQFFAS
                     VFGTSIQENYDLIRDIYVQIEPFKKRRAGQLSGGMKQKLALSCALIHKPEVLFLDEPT
                     TGVDAVSRQEFWHMLKRLQTKGITIIVSTPYMDEAGLCDRIALIQSGKILDIDTPADV
                     VGKFRSPLYAIKSRKTYLLMEHLRGSAETQSIFPFGEFLHYTDRRPGVSEQQLRDELR
                     TEGFDDVEVMRTEPTIEDSFMALMEQHETQ"
     gene            complement(58718..59116)
                     /locus_tag="PK937_00970"
     CDS             complement(58718..59116)
                     /locus_tag="PK937_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31176.1"
                     /translation="MKPLIIKLLISLSATIFGLATITAGGAAIFFNEGVFGTVIPWVV
                     WFNFFSGFIYVAASLYVWIDPRVAYKLSIYIFITTSVIFVLLGIHIASGLAYEKRTVA
                     AMGFRWIVWGIISLIGYYVFVKNQADNTKL"
     gene            complement(59147..60028)
                     /locus_tag="PK937_00975"
     CDS             complement(59147..60028)
                     /locus_tag="PK937_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNH31177.1"
                     /translation="MKTFITITLFLITLTACSKKNNTADAYGNFEAVETLIAAEAGGK
                     LLFFDVEEGVTLSEQQRVGCIDTTQLALKRIQLVASKQSVDARSANVLAQMEVLQEQR
                     RVAMTEKTRIENLYREKVATQKQMDDITGQVRVIEKQIASTEAQNAGVLSELAGLDAQ
                     IRQINDQIQKSLIINPISGTVLAKYVQSYEIVSFGKPLYKIADIRVMHLRAYISGDQL
                     SHVKIGQKVRVAIDDNATDNRSLEGEVIWIASKAEFTPKIIQTKEERVNMVYAIKVKV
                     QNDGSLKIGMPGEMYFQ"
     gene            complement(60038..60661)
                     /locus_tag="PK937_00980"
     CDS             complement(60038..60661)
                     /locus_tag="PK937_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH31178.1"
                     /translation="MDQNIKTEQDSAENKIIKAARSIFHRRGFDGARMQEIADEAGIN
                     KALLHYYYRSKDRLFQAVFEEAISRFFPLVLHMINSEAPLEQKIEKLIHTYIDFIKNN
                     RFIPGFVLHEMTTNPERMRRIFEERGIVPKLDVIKRQIREGIEDGRYIPIEPEQLLLS
                     ILSQCVFPFVAKPMVEFFFSMDEKQFNEFIERRKAQVTMLVLNGLRV"
     gene            60930..64181
                     /locus_tag="PK937_00985"
     CDS             60930..64181
                     /locus_tag="PK937_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31179.1"
                     /translation="MTKYVWRSLLFFEIALISLICSVTAQVRFVDLDRLSLEEGLSQS
                     SAICMLQDRQGFLWIGTEDGLNRYDGYSIKIFKADPADSGALQNAWITSLFEDHTGNL
                     WVGTRGGLHRYDAAKNRFISIPLDNTSLSVFAITEDLQHHLWVGTQQAGLFRKSVSTA
                     DWRQYRFDPADPRSVSSNDIFVLYTDDIGRVWIGTRDNGLNRYDAMADNFIRYQPTTG
                     NPYSISGLSIRSIRQYKTEIWIGTLNNGVNRLDPLTGRFNSYQPDASKPGSITHNSIW
                     SLFADSKDILWIGTFGNGVMQYDPAGDTFVQIQPKPSSTPRNDQVVVSILEDRSGMMW
                     FGTIGDGLRKYLRIKDRFQFINAHPNPKRGLPDNMIWSLLEDRQSNIWISTQKGITVY
                     NPKKDVFSQYTNPRREAWKLGETAYAMAQDSNGVIWFATFGDGLKRFDPRTQRISAFQ
                     VNKFSPGGIPTNNILALYVDHENVLWVGTYDAGLLRLEPGSRTFKVFKKKEKDSTSLQ
                     DNQVRTVFEDARGVLWVGTAQGGLHRFDRNSSTFKVYSTQSPGLKRLQNNFVSAVSED
                     KDGTLWVATYGGLHRMNRETETFICYSEKNGLASNQIYALIKDQRGRFWMTTSHGLSV
                     LDPHDAVPVFRNYTSHHGLQSDEFNMGAAIRNVSGTIMAGGVSGVVIFHPDSLESKKY
                     DVPVAITAVRVANRMLGYEPWDDKYTTLRHDQSTLAFEFASFDFSFPEQNKYAYRLEG
                     FETEWSDADASRRTVNYTNLDPGKYVFQVRGTNSDGHWSDKTAAFRFEILPPFYKTWW
                     FVGSSIFLIVFLAILFYRIRIRRIENQKNELARQITERTRELREKNSELERVNAIRNE
                     LLGMAVHDLRSPIAAIRNLVQFHLEALKNQINDRETDMHDMERALSLSDRISSTLNDL
                     LDISAIESGKLSITKLPENIETIIHDAFELNLRAAERKGTTLKSDIAPNLPKILVDRI
                     RIADVLNNLISNAVKYTPKNGRIRVYAEISGMFMHIHVEDNGQGLTQEDLSSAFKTFT
                     RLSATPTDGETSTGLGLAIVKKIVELHGGSVWVRSEKNKGSTFSFSVSLEGHH"
     gene            64314..65399
                     /gene="mqnC"
                     /locus_tag="PK937_00990"
     CDS             64314..65399
                     /gene="mqnC"
                     /locus_tag="PK937_00990"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNH31180.1"
                     /translation="MLQKIFDKIENNERLTFEDGMTLYACKELPLLGFLANHIRRKKR
                     PGNEVTYIIDRNINYTNVCVDLCSFCAFYRPIGHDEGYVLPTEVIDEKIDELIALEGI
                     QILMQGGLNPKLKITYYEDLFRHLRSKYPQIWLHCLSAPEIRYIAKVSGLTLDETVAR
                     LKAAGLQSVPGAGAEILVDRVRSEISRGKCDTQEWLDAHSIFHKHGLTSTATMMYGHV
                     ETREERIEHILKIRDLQDNSLKNYGLGFTAFIPWNFQPDNVPLGKNQNLRTTTTVEYL
                     QTLAISRLLLDNIDNFQVSWVTQGQDIGQLGLNYGANDFGSLMIEENVVSQNGTSFNV
                     TVKDIEKMIRDAGYEPKRRNNQYTFVN"
     gene            65499..65957
                     /locus_tag="PK937_00995"
     CDS             65499..65957
                     /locus_tag="PK937_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH31181.1"
                     /translation="MTKLSSKDVLRILDLQPHPEGGAYRETYRSKNPAKVDPQRSVCT
                     AIYFLVQAHQKTEWHRVKNDEMYHHYAGATLDVLLVSPDGVFEKHKLGDDLETGARPQ
                     LLIPGGYWQSVQAHDSWVLVGCTVSPGFDFSDFELSNEAALKKQFPHLSF"
     gene            65969..66934
                     /locus_tag="PK937_01000"
     CDS             65969..66934
                     /locus_tag="PK937_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH31182.1"
                     /translation="MTTQEVFINKNYQMRAGWRVLIFIVAFILISMPSVYLGLLFTKF
                     LEFPKEYNEIIVKFLTTIIPLGLSFFLLKKLDNRSTDYLGLKTDCAGFIELAWGLLIG
                     FVMLTIAVLIPYAMGYVSLQFATEPPSYFFSGLAFNLLLFIVVGFNEEILFRGYIFQA
                     TGEGLGFVGATLLYSLLFGAAHLGNPNVSLFGIINIVLAGVLLSLAYIKTRALWMPIG
                     IHIAWNFTQGYIWGLPVSGTTVIQPMIIAQEIGPDEITGGTFGPEGGIMCSIVCIGAC
                     LLIWKYFSPSDAMKQVIQEAETAQPFKPNISESIGSNTNPNNGIS"
     gene            67072..68172
                     /locus_tag="PK937_01005"
     CDS             67072..68172
                     /locus_tag="PK937_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH31183.1"
                     /translation="MNRNIFFLGVLFFSSFLFAHDGDIHKDSSSTTVRTITNIAPNIY
                     MIRHPDAPDGFPQGNTTVIIGDREVLVVDACYLPSSAKQDIEQIQKWTSKPVRYLVNT
                     HWHYDHTMGNGTYAEAFPGISIIAHHETKRIIQGYNPGWFARFPNRSAIFKSAIESGK
                     DANGNKLSESDIRDYKKYLDGLTPVQEEFSKIKDRAPNLSFDHELTIDLGNREVQIKH
                     LGRGNSAGDIILYLPKEKIIVIGDLMDHPVPYLGSGFPGEQVETLNKIAQLDADTFIP
                     GHGNVLTGKSYLFLLRDFLAEVVAAVRSEIEKVGNNPRLIDQVRESVHKRVDFTAWKK
                     RFVGDNKEDGDFFDSFSVQGVFNCAFNELVRK"
     gene            68554..69582
                     /gene="queA"
                     /locus_tag="PK937_01010"
     CDS             68554..69582
                     /gene="queA"
                     /locus_tag="PK937_01010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNH31184.1"
                     /translation="MKLADFKFKLPEKYIAQYPVEPRDKAKLMVLDRKTGDIEDKIFR
                     DITEHITKNDCLVLNETRVFPARLMARKEKTNAKVEVFLLRELENNLWEVLVKPARKV
                     RIGNRLVVDGVYCDVIDNTVSGGRVVRFIYPQADFHKFIDKVGQCPLPPYIKRSAEES
                     DKEDYQTVFARVRGSVAAPTAGLHFTKELLGRLKRKGVTIVPLVLHVGLGTFRKVQVE
                     DLSRHKMDSEYFEIPEITARAINQTKDKGGKVIAVGSTVVRSIESAVTSLNRVKPHRG
                     WTDKFMYPPYEFKIVDKMVTNFHTPESTLLMLAAAFGGKDNIFKAYKKAMKDNYRFYS
                     FGDAMLIE"
     gene            complement(69654..70076)
                     /locus_tag="PK937_01015"
     CDS             complement(69654..70076)
                     /locus_tag="PK937_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH31185.1"
                     /translation="MQNPYTQVTIEATIQASLEKVWSVWTSPEHIVKWNAASDDWHTT
                     KAENDLRVGGRFLSRMEAKDGSFGFDFTGSYDEVVQYELIAYTMEDGRKVRVHFSQSG
                     NSTKIVSVFDAEQENPVDMQKNGWQAILDNFAKYVVAV"
     gene            complement(70150..70794)
                     /locus_tag="PK937_01020"
     CDS             complement(70150..70794)
                     /locus_tag="PK937_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH31186.1"
                     /translation="MKIGVLGSGQVAQVLASGFIKHGHEVMMGTRDVTKLSEWKSKNG
                     DKAQVGSMATAASFGELIVLAVKGHAAEDAVKSAGITNLKNKTVIDVNNPIKEVPPVN
                     GVLQFFTGPNESLMEQLQVLAPEANFVKAFSCVGNALMVNPKMAEGTPTMFICGNNDA
                     AKAQVTAILAQFGWDWADMGKVEAARAIEPLCILWCIPGFTSNSWMHAFKLLKK"
     gene            complement(70890..72683)
                     /locus_tag="PK937_01025"
     CDS             complement(70890..72683)
                     /locus_tag="PK937_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31187.1"
                     /translation="MKLLIGYLKNYWKLVALALFLAAVNQIFSLLDPWIFRHVIDEYA
                     TRYKDYSFDEFVKGVSVLLGAAIGVAFVSRVAKNFQDYYINTITQKLGAQIYSDGLRH
                     SLELPYSTFEDQRSGETLGKLQKVRTDVERLISAFINILFTTLVGIIFVTAYAISVHW
                     VIAPIYFSTIPIIGWVSSVLSKKIKVVQKAIVAETTALAGSTTESLRNIELVKSLGLA
                     QQEIERLNGTTKKILKLELKKVKYIRSLSFVQGTLVNFLRNSILFIMLYLIFAQEITF
                     GEFFSLFIYSFFIFGPLQELGNVINIYREAEVSLKNFEDILNTPCEAKPANPVLIADI
                     EKLEFENVVFKHQSAQRHALDGISFSVHRGETIAFVGPSGAGKTTLVKLLVGLYKPQS
                     GIVRYNGHGGDTIDLDVLREQIGFVTQDTQLFAGTIRENLLFVNPKATDQECLDVLNK
                     AACGSLLARADKGLDTVIGEGGVKVSGGEKQRLSIARALLRRPRLLVFDEATSSLDSL
                     TEEEITHTIRDISHRQEHMTICIAHRLSTIMHADRIFVLEKGRIGEAGKHDELLAQKG
                     LYYAMWRQQIGENKSDMTVKNMMEVNSVRKN"
     gene            complement(72718..72942)
                     /locus_tag="PK937_01030"
     CDS             complement(72718..72942)
                     /locus_tag="PK937_01030"
                     /inference="COORDINATES: protein motif:HMM:NF022833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HNH31188.1"
                     /translation="MKFNKKHSQVLFVLFMALTLSCIMSLVMTFRTFGMETWHVALWL
                     QAWGFSFLVAFPVAFIAVPRIRRLVEMITE"
     gene            complement(72957..73253)
                     /locus_tag="PK937_01035"
     CDS             complement(72957..73253)
                     /locus_tag="PK937_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31189.1"
                     /translation="MNQYMVDVSLPEFPDEAFFSKIPEQRTLINRLLEERVVQSYALS
                     ADRTKLWIVIRAQSDSEAFDILATFPLYDYFRFDIYELAFHNKATVRSTLFSMN"
     gene            complement(73275..74129)
                     /locus_tag="PK937_01040"
     CDS             complement(73275..74129)
                     /locus_tag="PK937_01040"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31190.1"
                     /translation="MSNSIFIAGATGTTASQLLKHLATLGVKVKAGVRDIKKAETLAS
                     EYVAPVHIDYTLPSSIENALRGVKQLFMLTPFTDESVTMGKRIVDAAKKAGVQYIVKL
                     SAAGADAEPGITLVRWHREVEKYIEASGIPYTHLRPNSFMQNFINFFPPQNDVIYLPL
                     GEGKVSWVDVRDIAAVAAHVLTHSGHAGKAYTITGPDALSVTEVSKILSEATGKTISY
                     TDVPEEAARKTMVEMQMPNWAIDGLLELHAINKAGYAAEISNVTRDMTGKDPIDFERF
                     AKDNAARF"
     gene            74265..74624
                     /locus_tag="PK937_01045"
     CDS             74265..74624
                     /locus_tag="PK937_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH31191.1"
                     /translation="MKKQLPFCPVNATLNVIGGRWKPVILNLLREDTLRFSALKRQIP
                     GITQKMLTQQLRELEQDGIVRREVFPEVPPRVEYSLTEFGQSLKPILKAMCSWGSEHR
                     RYLDRKLKENRSVEEAA"
     gene            74630..75133
                     /locus_tag="PK937_01050"
     CDS             74630..75133
                     /locus_tag="PK937_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31192.1"
                     /translation="MKQPTRLHNFIIRKANIEDVPLILSFITELAEYEKLRHEVVATE
                     ALLQKNLFDNNPKAEVIIGELDGNPVCFALFFHNFSTFLGKPGIYLEDLYVRPAVRSQ
                     GIGRIMLAYLAKLAIERNCGRLEWWVLDWNKRAIQFYESIGATPMNEWTVFRVTGKNL
                     EELKDEF"
     gene            complement(75338..76201)
                     /locus_tag="PK937_01055"
     CDS             complement(75338..76201)
                     /locus_tag="PK937_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNH31193.1"
                     /translation="MRIDKILFPTDFSDCADAALEYAVLTAKKYKASLEFLYVDEATY
                     LLNPMGPYGDNDLHQLSDNVTSYADKKLDDTIQRLHHGVVGHKHVVIGRPYYQIVETA
                     RSIKADMIIMGTHGESGINAIIGSNAERIVRQAPCPVMTIRQKRDANHICRMLIPVDF
                     SDMCRQTMKQIIAYARDFACELTLVYVSVSHKNETEKGIQDDFERFLSRIDITGVIYR
                     TMMVKAHTEGAGIIECAVKNEIDVIAMGTHGRTGLKRVLMGSVTSEVVNNSPVPVVTF
                     RSVKQNNIQHN"
     gene            complement(76287..77771)
                     /gene="nrfA"
                     /locus_tag="PK937_01060"
     CDS             complement(76287..77771)
                     /gene="nrfA"
                     /locus_tag="PK937_01060"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HNH31194.1"
                     /translation="MKNIIQTVQEKPWVGWLLFCATVGVVFLVGMFANTVMERRGEEK
                     TLFQMVKPIADWEPRNEVWGENFPREYETYMSTKDTSFRSKHGGSATRDILAEYPNVV
                     ILFAGYAFSKDFKQARGHYYAVEDIRKTLRTGAPQPGTCWTCKSTDVPRVMNERGAPN
                     FYKEKWDNLGHEIVNPIGCQDCHDPKTMNLRITRPALAEAFKRRGMEVEKSNHQDMRS
                     LVCAQCHVEYYFKGKEDKYLTFPWDKGFTAEQMESYYDSVQHVDWVHAVSKAPMLKAQ
                     HPDYEIFKTGIHAERGVSCADCHMPYRSQGGVKFTDHKIQSPLNNIAGSCQVCHRESE
                     ETLRKNVNDRQDKVIEVRSRLEEVLAKAHIEAKTAWEKGATEHEMKPALQLIRQAQWR
                     WDYVAASHGGSFHAPLEAARVLGAGIQKAQEARIILSQVLMAKGVAWPITMPDITTKE
                     KAQAFIGLDMAKLKTAKDEFIKNILPEWDKKAAERQAKMGTKIE"
     gene            complement(77787..78413)
                     /gene="nrfH"
                     /locus_tag="PK937_01065"
     CDS             complement(77787..78413)
                     /gene="nrfH"
                     /locus_tag="PK937_01065"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNH31195.1"
                     /translation="MRKLRELIALVIPPDQWRFPVLILMGILFGLGLVILKISNAVSY
                     LSDDPATCVNCHVMAPQFATWQHGSHARVAVCNDCHVPHDNIIRKYFFKAQDGLRHAT
                     MFTLRMEPQVIQIKEAGAAVVQENCQRCHKNLTERVSAGRTSYAESRHGEGLLCWQCH
                     RETPHGRVNSLASTPYARVPRLAKVMPDWMESFLKNPQKTNLDTTLHP"
     gene            complement(79022..81061)
                     /locus_tag="PK937_01070"
     CDS             complement(79022..81061)
                     /locus_tag="PK937_01070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNH31196.1"
                     /translation="MLRQIILVITLFSAVLIAQDKKILTYEAFVDLRRVDEPAVSPDG
                     NWVLYNVRKYSLESNTYTSNIFLTSIDGKTSRQLTFSPTKDITPIWSPDGKTIAFLSN
                     RLGGFQIWTMALDGGEPKRVTNLSTQVSGNGLVWSPNGQYILITSDVFPDCETDDCNR
                     AHNDAAENSKVKAQLIGKLPFRVWDSFKDGKRSQLLLLEVATGKISDMIAGDYDVPPI
                     DLGGAHDFGFSPDGKTIIFTMNTDPRIAWSTNNDVWTMPVIGGEPKKISTSPGVDNNG
                     VYSPDGKYIAYCSMERAGYEADQTDLKLYEVATGKTKNLTEKFDRSVGTLVWSRDGKS
                     IYFDAEDQGFHPVFQMSANGGEPKKITAKTFDLLSDVAQGAIIVRRSSMRMPGEIFAV
                     REDGKKSMPLSNVNTAKLAALEMAAPEEFWFAGANNTTVHGFLVKPPKFDPAQKYPVV
                     YLVHGGPQNAWNDTWSWRWNPMLFAAPGYVVVMVNPRGSTGYGQQFTDEIRMDWGGKP
                     YEDLMKGIDYICSTYSFIDKDRIGAAGGSYGGYMMNWMNGNTDRFKCFVSHASIMNKF
                     NMYGGTEEMWFDEWEMGGPYWEGANRETFEKWSPYNYAQNFKTPTLVVHGELDYRVPV
                     IEGVNLFQVLQRKGIPSKFLYYPDEGHWILKPQNGRLWYQTVHTWFDQWLRQPAP"
     gene            complement(81445..82146)
                     /gene="pepE"
                     /locus_tag="PK937_01075"
     CDS             complement(81445..82146)
                     /gene="pepE"
                     /locus_tag="PK937_01075"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HNH31197.1"
                     /translation="MRLLLCSNSTNFGENYLDHWAAEIVDFLGAIKQIVFVPYALDDL
                     NAYTKKTRDRYAEMGIRVAGIHENDPRKMVTEAEAIHIGGGNTFRLLKKLYDEDLIDI
                     IRHRVSDGMLYLGASAGTNVATISIKTTNDMPIVYPPSFEALNLVPFNINPHYIDPDP
                     STQHMGETRDQRISEFHEVNNAPVLGLREGALIRREGDTLTLKGKNGAKLFTKGNTPQ
                     LNNTGADLSFLLRGF"
     gene            82266..83819
                     /locus_tag="PK937_01080"
     CDS             82266..83819
                     /locus_tag="PK937_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31198.1"
                     /translation="MCQFITLCLINLLFVFSSLQAGDGDKKPLSDVLKGIEKKHNVVF
                     SYVPALVQNVRVNTPSESDKLNHQLDYVFAQTDLTYRFIGTKEITLFKKTKTNSNIEA
                     TAQARLQAGRSRADEALSTMNKTLDGEQESGEHEDDSLPTDTTVSIDKNETSPSIVIP
                     TPMSPQTETQSVPTEKTEPHDLKNRSVRSEFLGMDFAPTTRIEPAYVPVPLHAISINI
                     ISTRLVSIEGVEVGLIGNYDRMYVNGLQIAGGGNRAYLHARGLQIAGGSNQTGDLSGV
                     QIATVNIADQTNGLQIGAVNISKKDHSGLQIGAGYNHAENVRGLQLASVNEASHVHGI
                     QIALVNRAKQVHGAQIGLFNFADENNGIALGLLNFIRNGQHHGRLARTERGFNHFSYT
                     HGSKYMYTIYDVAVRQLREPAQWALGGGIGAIVWPDRRLSLAIEATTHQHNKDKFWEH
                     RLNMVNTLKITPTIKLHKHLSVYAGASANVWVSRAENGFPAPTNAYNWDYKHTTIRAW
                     WGGLVGIYF"
     gene            83959..84495
                     /locus_tag="PK937_01085"
     CDS             83959..84495
                     /locus_tag="PK937_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNH31199.1"
                     /translation="MTTEEIQLLTKKIRNSDALAFRTLFDFFHRPLYHFVFYKTYDRA
                     LSEDIVQDVFIRLWENRFNLREDLSIKSYLYTIAVNLTLNTIRGHTIRTGEGIEDPDT
                     QPHEETPLSVLEKKEFQQLLNSAIQKLPEQARIVFMMSRYDDLSYSEISERLSISVKT
                     VEAHIGRALKQLRTTLKH"
     gene            85046..86002
                     /locus_tag="PK937_01090"
     CDS             85046..86002
                     /locus_tag="PK937_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH31200.1"
                     /translation="MSENYYHQDDFIGRWIAGTLTDEEKKEFELWLKSNSHQQEFFNE
                     LTKIHWSQKSLEIPKAPTERMWSAIAEATTSKQVSRSWIRNTTQWAAAIAASIVIYFG
                     LESQFAYREITTSKAQQTEIVLSDGSIARLNGLSSIRYNSWRWPLTRSVTMHGEVYFE
                     VQKMKRPFNVTAENLNVEVLGTKFNVRDRADRSEVVCSSGKVRVSARNNSENTILTPG
                     LAVSMHQGHFSMPYSMDTTLVAGWSQGIWNYQSAPIYSILNDWKITYGLTITMDSIDT
                     KRTFTGLIHGSSSLSALKQLGLSAGFETTIVNDSTFFLKLKK"
     gene            86016..88730
                     /locus_tag="PK937_01095"
     CDS             86016..88730
                     /locus_tag="PK937_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNH31201.1"
                     /translation="MNFYRVFFVAFFFTTYLTFAQEKEIASSSAQSAISLKQAVAELE
                     SRYHVRFSYVDQTIAKYTVRTPIKGDDLQQSLEYILIGKPLAYKRIGKNIIVLYEDDT
                     KKQEASGQFTSVRLTIVDARTSEGIYFANVTISGSRRGALSDAHGQAVVQHIPYGDYT
                     LRITALGYLPLDKTLHLSSQEITLDTIRLEPSDIRLDSTIVFGERETWRVSDAKLRVQ
                     PSIIVIDRRKINSTPSVLEPDLFRTLQTLPGVTAPNDLSNELYVRGGTPDQNLITMDR
                     AIVYQPYHLFGIAGIFNTDAIEQVNFSTGGFSAQYGNRLSSVIDVRTKSASPNKFSAT
                     GNASLLSSKITMDGQPSKHWYYLISLRRTYLDFATKAAVWLKIAPESIPYSFYDGLSK
                     VVYRPNTLNEFGTSLFFSRDKFFSKHRDYEYVFNEFNISRKLPMYSVGRLDYGWGNLN
                     AAVYWDWQAGDIFRSRVTISQSKATIKLDEDLYYGYDSGAPDSIRRRTDSLNALQEKR
                     PFDTRNYIVDRGFAWDTQWEWLNEHSMYLGVSLNLIEMSYYWKNFNAPPHYVQVFYDF
                     APDSFRYQRRTTNLAFYIEDMWKKGDWTIKPGIRIEHFSSNKRSLLISPRTAIRYDVS
                     PKLALKGSAGIYYQSLFTARERGYIGFLEVPFSTEDQVEKATHIIAGGEFFPNPNIRL
                     TADIYFKHFNRLYRKDHSSDSLHFDKGGGDAYGLEITYRRIGNKMSFEADYSLAWVTR
                     TFDQNTYVTNYDQRHTLNLLLSYRLPKNWTVDARWILASGRAYRPTEFYGATAYIEPG
                     SGRSLMEDNGYNISQENLDHLEFYDRYPLYHRLDVSLVKTVRFKTWTMKPYISIINTY
                     YSNNPLFYDYNYNGSIISNEPDPQRMNYSKRKGYGVPILPSFGTFFEF"
     gene            88733..89668
                     /locus_tag="PK937_01100"
     CDS             88733..89668
                     /locus_tag="PK937_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNH31202.1"
                     /translation="MKKKSMTFVMVALFVGSACNQNNNNEFLGVFGILMPYASENATI
                     ILMRTYSQENYDEFIPEALQIKESNAEVIISSDSQHVTLSEVIPGRYVDTAGMLKYYP
                     GLIYKLEVKAKDGLSVHAQCVLPGVPIPDPETTPDSFSVAIELDTVRRIVSGYPYPVL
                     TQMYYLRSPTVSFDWSDATGAEAYYWEIGADTSYLKESDREFFIPNSKLFQTEKWIAY
                     SSLVLFDHWDSTTYRGLYNKPDNPWTWSGFYIDSEREFVYTSQLHITAIDRNFIDYTY
                     YNHKSNIRGGYGYFSAATRHKRPITVHVQSKWKQR"
     gene            89734..90780
                     /locus_tag="PK937_01105"
     CDS             89734..90780
                     /locus_tag="PK937_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31203.1"
                     /translation="MDTSSGDKQPPHFEWDPGSATVILKDMIQYGENNIAILSLHYDT
                     NRYGLTGFTPNGIQKFQKFLPATHYIESSYFERNLSYSNGIFTLVINPKDTGQNSLFI
                     QLDTNGNITNQKELVQIKYASIFPMNNGEYLVRSSIFDSLQFKTKLSKIDNDGELKWE
                     KVMPVSFEYLHVANNRIYGKWNNDSIVSIDANGDVLWKQSLMLPKGFLLCRVSSSDYR
                     IARILYNPYNYIPDRLAIDAMDIDGAITPLDTIPLNWSTDFTDFESLGANELVLLGRW
                     GGSNKILLMRLQSDGSWVESHLAPDENNFSLVSFHANGDGTYLIMGAMYINSSYYSKT
                     LIYKIDANGNYLPF"
     gene            90987..93170
                     /locus_tag="PK937_01110"
     CDS             90987..93170
                     /locus_tag="PK937_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNH31204.1"
                     /translation="MSEQEILRLVAKELNIPVQHVTSTIQLLDLENTVPFIARYRKEV
                     TGGMDENQIRDIQDKMTYYRALEARKEVVLKSIEEQGKLTDELKARIVAATMLQEVED
                     LYLPYKPKRRTKGIIAKEKGLEPLALQILAQEITSGTLDEHAQPFINLEKGVTTVDEA
                     LQGARDIIAEIISEDADVRKDVREFTRENGILVSEVKEDPENEYEMYHTFSEPIAKMP
                     PHRILATNRGEREEKLRIKITVDEEACCNIIGYRHVKKQKSIFRDQVIEAIKESYFRL
                     IAPSIEREIRGEFTEKSEEHAIEVFGSNARNLLLQAPTKGKIIMGIDPGFRTGCKVAV
                     IDETGKYLEGETIYPHEPQKDALGSKRVLKQFIRKYEVSVIAIGNGTASRETESLVAD
                     LIADLKKEDSHLELVYTIVSEAGASVYSASKIAQQEFPDLEASQRGNISIARRLLDPL
                     AELVKIDPKSIGVGQYQHDVNQKRLAEQLTHVVESAVNYVGVNLNTASAALLTYVSGL
                     SSKTAQSIIAHRDKNGKFSRREQLMDVTGIGPAAYQQCAGFLRIPDGDNPLDNTSIHP
                     ESYDATQKLLKKFNIVDPSTAGSLLELQLRNAKMSFENIAGEVGVGVPTLKDILDNLK
                     KPGRDPREEMPKPIFKSDVLKMEDLREGMMLKGTVRNVVDFGAFVDIGVKQDGLVHIS
                     HLSNKYVKNPMEVVAVGDVVDVKVMSVDLKKGRVQLSMKEASRSY"
     gene            complement(93244..93816)
                     /locus_tag="PK937_01115"
     CDS             complement(93244..93816)
                     /locus_tag="PK937_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNH31205.1"
                     /translation="MDLTKELKDNVLLTTVEAVYDWGRRNSLWPMPFGTACCAIEMMA
                     TLGPRYDMSRFGSEAIRFSPRQADLMIVSGRVSIKMMPVLKRIWDQMPEPKWCISMGA
                     CASTGGFFNTYTLIQGVDQFIPVDVYVPGCPPRPEGLIEGVMKIQKLIAKREAAYARD
                     IKDDGMYGDDKVGIAGRGKDLVAEIATGKF"
     gene            complement(93850..94455)
                     /locus_tag="PK937_01120"
     CDS             complement(93850..94455)
                     /locus_tag="PK937_01120"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNH31206.1"
                     /translation="MAFTLPSLPYAHDALEPHFDKMTMEIHHGKHHNAYVTNLNNAVA
                     GKPEENLSIEEICKNISKYPVAVRNNGGGHFNHSLFWTVLGPNAGGEPTGALADAIQS
                     AFGSFADFKTQFANAGATRFGSGWAWLIVKEGKLAICSTPNQDNPLMDIAEVKGAPIL
                     GLDVWEHAYYLKYQNRRPDYITAFWNVVNWNKVAENFANAK"
     gene            complement(94534..94947)
                     /locus_tag="PK937_01125"
     CDS             complement(94534..94947)
                     /locus_tag="PK937_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNH31207.1"
                     /translation="MRVHTHYLWFNTKQRREYINITDEVESALNVSGIKEGMALVSAM
                     HITAAVYVNDAEDGLIKDIDKWLDHLAPANPNYLHHRTGEDNADAHLKNLLLHHEVIV
                     PVTGGRLDFGPWQQIYYAEFDGQRKKRVVIKIMGL"
     gene            95038..95925
                     /locus_tag="PK937_01130"
     CDS             95038..95925
                     /locus_tag="PK937_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HNH31208.1"
                     /translation="MLKSYIQLPPDFSFDECLVFLNRSDREILHRVHNKSLTKAFRIG
                     HETCLTSITVKSDKMMLRFERDISNEAARFIRSEVSEWFDLSCDLANVYRLMKKDPKL
                     FALTKRFHGLRLIGIPDLFEALAWAVIGQQINLAFAYTLKQRLIDVAGQSLTHDGLPY
                     GLFPDPATVADLRVDTLRKLQFSTRKAEYLIHIARQIADKRIEKTALQNYSLSQIVER
                     LTAERGIGAWTAHYVAMKCLRQANAFPIGDAGLHQAVLHIYQMKKKPTLIQIEKKSKL
                     WQGCEAYAVFYLWRSLQET"
     gene            complement(95932..96678)
                     /gene="lepB"
                     /locus_tag="PK937_01135"
     CDS             complement(95932..96678)
                     /gene="lepB"
                     /locus_tag="PK937_01135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH31209.1"
                     /translation="MTSSSDVSSAGESNAVEKPLSDNHNKTTELFQIAFVSVFIAIIV
                     RLFFVQAFKIPTGSMEDTLLVGDLILVNKCIYGVKTPATIPLIFTDIDLAQYQFPAVR
                     EPKQGDVIVFKFPPDPTVDYIKRLVALPGQTVEIKDKIVYVDGKDFSEIIRPPGLKFE
                     DPEIIPRNKGYESVFPRDAGSRDNYGPVTVPEDSYFVLGDNRDRSYDSRHWGFVPKDH
                     LIGQAVMIYWSTASQDNFDIRWDRLGQMIH"
     gene            complement(96710..97645)
                     /gene="lepB"
                     /locus_tag="PK937_01140"
     CDS             complement(96710..97645)
                     /gene="lepB"
                     /locus_tag="PK937_01140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNH31210.1"
                     /translation="MSIFGKKEEPKKNNPVPGKPLKEMTFTERLVENVKSFGWALCVY
                     YITITFVVQNFTIPTGSMEVSLLPGDFIFANKFIYGAKSPENIPFTDIKLPQFQLPAF
                     KEPQQGDVVVFRFPHPEMMPSQNGLDYIKRCVAAPGQTLYVRNKVLFVDGEALSVKFD
                     LPGVHFDPYERSEGQVFPRHMGSGDNFGPFRVPKKGDQIELNLTNYELVRYLSLRDGK
                     KLEMRGGEWTVDGVKSTYYTVGQDYFFMMGDNRDNSLDSRYWGPVPRSHIAGQGMLVY
                     WSNTDAAKQSSIPMKVLMMFNFFSVKWHRIGTIIR"
     gene            complement(97647..98132)
                     /gene="coaD"
                     /locus_tag="PK937_01145"
     CDS             complement(97647..98132)
                     /gene="coaD"
                     /locus_tag="PK937_01145"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNH31211.1"
                     /translation="MRRAIYPGTFDPLTLGHLDVIARAATLFDEIIVAVANNSKKKPL
                     FSPEERVAMIAEATKDMANVRCEPSEGLIVDFAVSRQVHVLIRGLRVISDFEYELQME
                     HVNRRLNSQIVTMFLMPGEKYAYLNSTIVKEVAQYGGDLSSFVPAFIEQRIKEKLKVQ
                     K"
     gene            complement(98135..98698)
                     /gene="rsmD"
                     /locus_tag="PK937_01150"
     CDS             complement(98135..98698)
                     /gene="rsmD"
                     /locus_tag="PK937_01150"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNH31212.1"
                     /translation="MRIIAGIRRGLVLNAFDEEIIRPTKDRVKESVFNILANIIDVTD
                     TQILDLFAGTGNLGIEALSRGAAKSTFVELHSKAVHVLRNNVIKCRFEQQIDVITGDA
                     LEFISSVAVKKYDIVFADPPYAMRIGAMIAKKIDAGQWLNPGGVLVIETSPEEIENEW
                     LPAHGLTMHKQKTWGESAVSILMNKAN"
     gene            complement(98761..99747)
                     /locus_tag="PK937_01155"
     CDS             complement(98761..99747)
                     /locus_tag="PK937_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31213.1"
                     /translation="MIKINLLENHFAPPAQHEKTDSDTSAGKTITIEAKKSQRRLLFI
                     ILIFVALVGGLTYGWMERYAVVAWLEQYTGPLHLLPEQDMETAAKAAEEARQEKIRNL
                     YMYNTIRYQERYRRFLFRTDSIVSANSQKWHLTSLGVEESNYAFDVFGKTQKDLEDFT
                     VLFLESKSIDETKPQAIKPTSLVKGLGFKRTMSGTLKTVSTPSDSEIVALKIIYLDLE
                     KAKQKILDGARNAGLKILQDGKMTSSKGVVMSTHSGELKLEGSVSDYFKYMAKLNEMR
                     FNFEIGEIDIQYADYSARRKADKKKLVPDTIFMKYSILLPRQSESADSTKTK"
     gene            complement(99749..100837)
                     /locus_tag="PK937_01160"
     CDS             complement(99749..100837)
                     /locus_tag="PK937_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31214.1"
                     /translation="MSQMSIQSDRGDLDHDAIDQDYARRAGEYRNLAFGLSFHGQQIH
                     FAEILRSEDGFSVRKMGTIQTTMKFGTGIEGVERNVQGLFAYLKGCLEDNGIRAKRMN
                     ISLNTNLVTLHKVLVESERSESDFQDYIKWEFRQQVTDDFDQYILNTVPLEKTSPAPM
                     DPMLMVGIRRRYADSLRSVLDKSKIQFSNMDVDILCTHATYEVNYDISYHGMTVLAEC
                     KTGSVTLLLCKDYDVQHVYQFTVSPKSTPQKIGELINAHIPNMMQAYHATAGNTNSVV
                     GRLILCHDLAQEVLPYIEADFNPSVIEPFRKIKLPAEFRPHEKNEDSESASADADEKD
                     NKSQVVIAPPDYAVYAEAVGAAIKLLMD"
     gene            complement(100853..101392)
                     /locus_tag="PK937_01165"
     CDS             complement(100853..101392)
                     /locus_tag="PK937_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31215.1"
                     /translation="MASIPKTRITDYILKTIIVLLFVFLVWSVVSPVQAEKADRNKIR
                     LTRAKMKALLAVQKAYFVSDSSFTSDNGKLFDFVNKKGKDKVPDEVFTPVIEALMRFP
                     EYSEYFKDVTPATYREQYFESLTDNPMNGQKFIIETGLKDGRKTVHIKPSSVDSEIQR
                     IGAVIDSRVTWNEKLELTY"
     gene            complement(101396..101926)
                     /locus_tag="PK937_01170"
     CDS             complement(101396..101926)
                     /locus_tag="PK937_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNH31216.1"
                     /translation="MNFRNWGANAYKFFEYFGLTRQEAVVLCVLAGITLLGGLVGLIK
                     ETYRDDTIEITSVPYPKNEARPYPQKSENDSAFSVWFSRQDTLQTLFRKGVARDSIQR
                     FILKTNDAPARVVRINQADVQEISSLPGVGPKIAARIIEYRNKNGPFSKLEDIQNVKG
                     VGKKLIEKIRPFIDIN"
     gene            complement(101954..103090)
                     /gene="dnaJ"
                     /locus_tag="PK937_01175"
     CDS             complement(101954..103090)
                     /gene="dnaJ"
                     /locus_tag="PK937_01175"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNH31217.1"
                     /translation="MAKRDYYEVLSVSKNASLDEIKKSYRKLAMQYHPDKNPGDKSAE
                     EKFKEIAEAYSVLSDEQKRAQYDRFGHDGMRGMGQQGFSDINDIFSHFSDIFEGFGFG
                     DAFGGGRGRSSRSNRGADLEVKLKLTLQEIAEGVTKKIKLRKRKSCESCNGSGAAAGS
                     SRKTCSVCHGSGQVRQVSRSVFGQFVNIAACRNCEGQGTIVEKPCSTCKGDGVTMGET
                     VISVKVPAGVTTGNYIPLRGQGHAAPHGGESGDVIVVLEEIEDEIFERHGDDVLLNVN
                     ITFPQAVLGDEVEVPTLFGKAKLTIPGGIQSGKILRMRGKGIPHLNGSGSGDQLIRVA
                     VHTPSKISDKERRLIEELMGYDNFRSPNSNDTSFFKKIKEAFTA"
     gene            complement(103243..103776)
                     /locus_tag="PK937_01180"
     CDS             complement(103243..103776)
                     /locus_tag="PK937_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31218.1"
                     /translation="MLFGKKKIHARPGALDADAVKAAIKGLAVNDGATVERVDWDGSR
                     STVSGRVSHVATDYTSFVLVEEGSGESIEFYIEVGDIVKLDRVAISIDLDENQQNTFM
                     EVAQIIELLEALDHGDHIAVSYFDVIKGRGVSVKGIITLKDEYNKVFAVEYEENGEKK
                     DKHFNLNEDKIIDIIME"
     gene            complement(103817..106267)
                     /locus_tag="PK937_01185"
     CDS             complement(103817..106267)
                     /locus_tag="PK937_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNH31219.1"
                     /translation="MKNNFSSRVQLVVKLSQEEAIRLGHDYIGTEHLLLGLIREGEGV
                     AVKVLKNLAMDLEKLKRAIEDMVKPVTDNTVIGNPPLTKRAEKILKSTYVEAKNFKSD
                     VIGTEHLLLALIKEKDCLAAQVLSMFNIDYETVRRELTNIMSGTQTPTNPTAKKPEPK
                     KSKTPALDHFGRDLTQYAEENRLDPIIGRDEEIERVAQILSRRKKNNPVLIGEPGVGK
                     TAIAEGLALRIVQKKVPRVLLGKRIVTLDVGALVAGTKYRGQFEERVKAIMSELEKTT
                     EVILFIDELHTIVGAGGASGSLDASNMFKPALSRGELQCIGATTLDEYRQYIEKDGAL
                     ERRFQKVIVDPPSVEETLEILRQIKHKYEEHHRVTYTDEALVQAVRLSDRYITDRHLP
                     DKAIDVMDEAGSRTHIMNIVVPEEIVALEQEIERVRTEKDFVIKNQKFEKAAELRDVE
                     KRLTEQLRVAKEEWEKKEDTQVVEVGEEQIAHVVAMMTGIPVNRVAQSETEKLLKMEN
                     VLCSRIIGQDDAIQSVTRAIRRTRAGLKDPNRPIGSFIFLGPTGVGKTQFAKELAYYL
                     FENADALVRIDMSEYMEKFSVSRLVGPPPGYVGYEEGGQLTEKIRRKPYSVVLMDEIE
                     KAHPDVFNILLQVLDDGILTDSLGRKVSFKNTVIIMTSNVGAREMSKIDGFGFSTKDN
                     GEDEYLRMKAKAEEATKKLFNPEFLNRIDEVIVFRALTKDNVLRIIDVSLDEVRKKLK
                     ERNMTLHLTDAAKEFVATKGFDPVYGARPLRRAIQKHIEDAVAEKILKGEFSDGSLVS
                     IELRDGELAFVEMAHEAV"
     gene            complement(106485..107330)
                     /locus_tag="PK937_01190"
     CDS             complement(106485..107330)
                     /locus_tag="PK937_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH31220.1"
                     /translation="MAHYVEHNNDLIDKYLQEIGAVPLLTSEEEIALAQRIREGDERA
                     LEHLTKANLRFVVSVAKQYQGQGLSLNDLINEGNIGLIKAAKRFDETRGFKFISYAVW
                     WIRQAILQALAEQSRVVRIPLNKIGTLNKIGRVYSDLVQEFEREPSAEEIAEQLEISE
                     LEVHDTLQMSGRHISVDEPIYDSDNNTLIDVIENEQQAPPDHDLVNESLKIEIERALD
                     SLSARESEVIRLYFGLENDHPMTLEEIGVRFQLTRERVRQIKEKAIRRLRHTSRSHVL
                     RHYLG"
     gene            complement(107363..107620)
                     /locus_tag="PK937_01195"
     CDS             complement(107363..107620)
                     /locus_tag="PK937_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNH31221.1"
                     /translation="MARIIGTVWATKKHPSIEGCRLQIIQPVDSELKHVGGPIVATDS
                     VGAGQPNELVYYVTSGEAPIPLDRKAPTDATIIGIVDRIDR"
     gene            complement(107623..107985)
                     /locus_tag="PK937_01200"
     CDS             complement(107623..107985)
                     /locus_tag="PK937_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNH31222.1"
                     /translation="MANKINLADKFETFSDTWHPRIVGELNGQHVKLVKVSGEFVWHH
                     HDHEDEMFYVVRGCLKMEFRDRTEIIDPGEFIIVPRGVEHRPVSDVETEIMLFEPATT
                     VNTGNIKNERTRENLQHI"
     gene            complement(107985..108254)
                     /locus_tag="PK937_01205"
     CDS             complement(107985..108254)
                     /locus_tag="PK937_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNH31223.1"
                     /translation="MVLGKVIGSVWATRKDQKLTGVKLLIVRSIGLDYKEKDAFVVAA
                     DSVGAGVGEVVLYCTGSSARQTEATENRPVDAVIMAIVDKMEVHP"
     gene            complement(108261..>109310)
                     /locus_tag="PK937_01210"
     CDS             complement(108261..>109310)
                     /locus_tag="PK937_01210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNH31224.1"
                     /translation="GRLILLSIQSIDGVYMKNKLRRLSQSLREFSERLSLAGMETQTT
                     ACLKEQIQNTLRFDRMHIWLRHGAEEIRLVEAGGSAAYDKESMVRLDGTMIEKVMTKR
                     RRLYISDVTSIDRTSVNSELELNYLASVVVIPICDQDHCFGVLVIEQGQKNAYDVYEV
                     QFLETVCNSAGLALARIALDQHLSKNANEGERNASTVEQTFMKNVVSEIHRAKRFETS
                     FSVLIFQVDYVKNIYEAFGVEAGDLALEKITEILKNGIRFIDSLARLSHDRFGVLLVE
                     SKRKEAVECAGRILHALESAVIHSGDEEIPLTATVGVVTYGTDGTEADALMHRANVAL
                     VNAKKLNERRIACAD"
BASE COUNT        30224 a        25686 c        22841 g        30559 t
ORIGIN      
        1 tggcttagat ggcttagatg gcttagatgg cttagatggc ttagatggct tagatattcc
       61 ctataatacc cataatatat cctacgataa gaatataaca aaaaaagcag ctgaaatctg
      121 taggtatttt actgaaaatc ttgtaggtaa tttacctaca agatttcgct aagagtaaaa
      181 aaatcaacaa aaacagatag cctttcaaaa atatttcata ggttacaaaa acaatctctc
      241 ctgctgattt gcctaaagag tcagtggccg cctaaaaaaa taaaagatgg ggatgatgta
      301 gtcatataaa atcttcggcg aggttctaaa cctctatatg tatcaatttt ttacaccctg
      361 cattcttaca ggaaattcgt tagattgact ttccataagg cattacggtt agctcaactc
      421 gtgcctttaa aataatacat acaatttcaa aataattatt taattttagt taaccgacct
      481 tacgtatatc aagagatctt tacatggaaa catcatcctt atttaccatc atcgccgcat
      541 ttctctcgca atcacccatt gtcgtcgtat ggattgtcgg cgcggtattg gcttatcggc
      601 aaaagcctgt ttatccgcga aaatcgctgt tgatcattat tgccatgttg ctttttctcg
      661 gccaactttt tattaacacc attcttaata tcgtattccc gatgatgtgg gatacccagc
      721 aactgctcct gagctacaac gtcaaagcga tggttacatc catgatcatg acggcgggct
      781 ggattttgat tatgatggcg ttatttatga aggaaaaatc ccgtgacgga ttcggacaaa
      841 actgagaaaa tttaccggat cgcgagaaaa ggttccaagg tttgtaagcg ctcgtcgttt
      901 ttataatata cccgttctaa aaacaatccc gacggcggcg ccgtgagacg cgccagtgac
      961 ggcgacgttt gacgaaaaag gcctgcgata tcgcccggct tcatattacc gcgcccgact
     1021 tccgccagta ttcccgttat ctgacgcacc atcttccaca aaaaatgaga accgacgacc
     1081 cgtatcaaaa taagatcacc ccgcgactcg atgcgcagtt cttccaggtt cactttggtt
     1141 gaatcggcct ccccgtcttt ggccgcaaag tttttgaaat ctttgagccc gacaaaaaaa
     1201 cgggacgcct cttgcatccg ggcaagatca agttcgtctt tgatccacca cacaaaacgt
     1261 tttccgaacg cactgcgacg acgggaaatc tgatagagat agctccgcgc gacggcatca
     1321 tgacgcgcgt gaaatgaccc cggcgctttt tcgatttgtt tgatgtgaat atcgtgcggc
     1381 aaacgatcat tgaccgcgag gcgaaattta tacggatcca gaacggtcgt acaatccagg
     1441 tgggccactt ggtgcagtgc atgcacgccg ctgtccgttc gtcccgcgcc ctgaaaatca
     1501 aaatttttgt ttcgcagctc ttccgtgatc gcgtacatca gcgtgccctg gacagtatgt
     1561 gtatctttct gcacttgcca gccgctatac cgcgtgcctt cgtattcgag cgtgagtttg
     1621 tatcggggca tgatttaaac aagaaattgc aaatcaatat ctattggatc gtgattcgca
     1681 taaggaagga attcgggaca cgaaattgtc attaaccact caacttgaaa aataataaag
     1741 atttaaactt gctcgtgata tgctgagttt gtcgaagtat caccacgcta gacgcaatat
     1801 ccgagttgta tgaaacctcg gtttctatat ctctataatt ttttgtttaa aaaaagaact
     1861 tcagaaggtt tccaaccggt cgcatttcgg tcagcgagtt caagccaacg ttttattctt
     1921 ttgttaggcg gaacagattc tttccaatac tctacaaata aagcgtcttc cattgttttt
     1981 gcggcattta tcatttcatc ttcaatgtct gataacgccc tgtgaatata caatccacct
     2041 cgaatactgt cagaagccat tgcaacactt cctgctgaaa ataaatctct tgcttgggaa
     2101 gccaaaacac cgtgataagt taataaatac atagcaacat cattgtattt atttttcaca
     2161 cgcgaacccg caacaatttt cggacacaat aaagatgctt ctgctctcag cttccttttt
     2221 tctgattgcg aaagctccgt gtatagtcta acctgaatag gcaaagtgtg ttcttgttcg
     2281 ggtttttctt ctatccacca aagacgttcg ccttctttta atcttgcctt tgcgtattct
     2341 ctgatataac gcattttctt ttcaattggt aaaacagaaa agtcatcgta tgaaattttc
     2401 atttgctcaa ataatgaaga tttcgcgtct atttctaatt caaacctcgg aacatgcgat
     2461 gtcctaacgt gaattacgca ctcctgatat gattgccatt ttacctccgg caaaccaccc
     2521 attttaccaa acacaacata aataaataca acctctttgt ttcggaatgt ttcaaataca
     2581 ctgttagcaa cacttctcca attatctttt tctgtgaatt ttacttctat tccgtactct
     2641 ccaacggcaa tatcgggaaa tgcataaggg tgcggattgt aatctatttc aactttacta
     2701 tctgtgataa gagttttaag aatgtctctt accctattct caaatacttt tgacgaagaa
     2761 aatacatttt tttgcgcttc gtacgttaat tgttgcgcca atttgttaag aatatcttca
     2821 aattcttttt ttttcatctt acaaaagaca gtcttttact gattgcgcaa tgtgccaggc
     2881 caatacaggc gggacagcgt ttccgatttg tcgttcagtt tctctaagtt ttgcttcaaa
     2941 gataaaattg tccggaaaag actgaatacg cgcggcctct ctcatagata atctacgtgg
     3001 caatgaatag tgaaattgaa tattcccgtg acattcagcc ctaattgtga aagcgggctt
     3061 tgatggctct aatcttcttc cgccttgctc gccgcttttt tttgctaaac tccatatatg
     3121 gttaatctgc tcattttcat ttaccttttc caaatcgtga attgcgtcat aacttgtaag
     3181 ccaattttct ttttgtctaa ctgttttagg cgggacaaac gacttaacgt cttttgccgt
     3241 tccaacaata aaaacacgct ctctactttg tggaacgcca taatccgctg aatgatacaa
     3301 ctgataactt atattatatc ctaactcatt aaaatcgtga agaatcttat taagtgattc
     3361 tcgtcgcgtc ttcattaata atccttttac gttttccgca acaaatactt ttggattcgt
     3421 gagttttatt gcttctatca tataagtata aaggctactt ctttttccat ttacacccaa
     3481 accttttccg tttattgaaa tgtcttggca aggaaaaccc ccaattaaaa catctgcttg
     3541 ggaaggaagt tttcctatat aatcacgaat atctccgcaa tgaatattac tgtcaatatt
     3601 tcttgaataa cttttacaag catacggatt tagttcatta gcccatttaa tttcaaatgg
     3661 atttttataa tattcatttc cgaaaattga aaatccgcct ataaaaccca aatccattcc
     3721 tccgcaacca gaaaacaaag aaactattga aaattttttt ttctttttca aaatactttg
     3781 cgaaaccctt ctttgcattg ttttcaactt aacgattgtc gtttcgttag cggcaatcgt
     3841 tagaacgtca ttgtccaact ctgcatattg ttctcttatt gctgttttct gcatatcaat
     3901 tatgcctttg ctttttgtaa tacttcactt tttcttccgc aattttgaga atgtcttttg
     3961 tataatcttc ttccattatt tcataagcga ctttatcgct taggaaggaa acaagtaatg
     4021 gctttgaatt aacggaaaac agttcggcta atttttcaat tatttccttg ttcgcgttgc
     4081 gttcattcct ttcaatcttt ccaagagttg acggatcaat atcgagatgc gccgctactt
     4141 ttcgaagagg caaaccgctt tcttccctta gttttctgat tgtgtctcca aaagtttcat
     4201 tttgcatcat aatcatgaat agtatataga aaattgattc aggacaaaca gtgtccaaac
     4261 atacgatgcc cttaaaaagg aatcaacaat tttgtctttt tataaaatat ttttatttaa
     4321 aacctaaaat tcggagagtg aatgagcttg gttgcctcgc ttttctgtct gtcaaatgta
     4381 actttgatga tgactcattc tatgggcgcc cgtatctcaa tagtcgaaca cattaaaaca
     4441 agaaaacgaa aaaaggaaag aaaaaatcca ccaacaatcc tgctaattat ggacaatcct
     4501 attatcgaac agcagcttgt ttaaacattt taaatcaacc caatttgtag attaaaaaac
     4561 tccgagccat gaccggctcg gagttgtaat attaattcaa aaaatcagat ccgcgatcac
     4621 atgccggtaa tattatatcc gcagtcaacg tacatgattt caccggtgat accgcgcgaa
     4681 agcgggctga ccataaacag cgaagcgtcg ccgacttcgt ccacttccac attgcggcgg
     4741 agcggcgctt tttgaggcgt gatatccagt attttggtaa atccggaaat gccgcgtgcc
     4801 gctagcgtat tgatcggtcc tgccgaaatc gcattaacgc ggatattgga cggaccgaga
     4861 tcggctgcca gatatcgcac cgaggcttcg agcgatgctt tcgctacgcc catcacgtta
     4921 taaccgggta tgactttttc tgcgccatag taggtcaagg ctacgatcgc accgcctttt
     4981 tctttcatca gcggcatcgc ttcgcgtgcc ataccgatca atgaatacgc gctgatgtcg
     5041 tgggctacgc gaaatccttc gcgcgaagtc gtactaaacg gtttctctaa atcgtctttc
     5101 tgtgcgtaag caatggagtg cactaaaaaa tctatatgcg gcatgtcttt gctgacggct
     5161 tcaaacaacc gcttgatatc gtcatctttg gtcacatcgc aatcataaag cagattactt
     5221 ccgagttcgg ttgcgatttc ttcgacattg cgcttgaggc gttcaccctg gtacgtaaaa
     5281 gccagcttgg cgccttcccg tgcgaggctt ttggcaatgg catgggcgat actgcgctta
     5341 ttggcaacgc cgcaaatcaa tccttgttta ccctgcatca gcataatgat agtactcctg
     5401 attgttagtg taaaaaccgg cggaaatata gccaaacgaa aattgcataa caactaaaag
     5461 attcccgtca ccggtcggac gcctcaaaac ggtacggcac cgacgtattt caaaaatcca
     5521 cactgatcac ggttcggctt ttgggcacct gacttttgag agcgccctga tgcatcacgc
     5581 cgcatccgag cgccgttcct tgcgaccgga ctaccacgcc gccggccata ccgggaaatt
     5641 cacggcgtat cgtccctgct tcgacaaacg tttcaatttc cctcgtatcg gtcaattcga
     5701 taatattacg ggaaatgtcg cggctaaaaa gttgcgccgc gttggtcgaa agtttccaca
     5761 tatcgccttt acgggtgcgt gccagacgca tccccacacg atgggtaaaa agcgcattga
     5821 gcaccgattc gcgaccgcgc caatcttccg atgcgatgaa tgtttcatcg tctttgatgt
     5881 gataaacaaa ttgatcccaa aagttttcat caaaaccgaa aaggccggaa aaatattttc
     5941 ccatgccgcg gatttccgca tgcgaggatc catccagcgc atacattttt ttgtgttccg
     6001 cttgcgccgt tttaccggtg cgcacttcga tggcctcatc cagtctgaat ttggcaagga
     6061 aaaatccttc gggattgatc gctgacggca tcacgcgacg ggtcagcgct acgcgcgggt
     6121 caaatatctt ttcccccagc gccgtaatgc ccggcagtgc ggctataccc tcgggcagat
     6181 caaacgcttc taaatgcaaa gacggatacc gtttgagtat cgtatccacc aatccttcat
     6241 tttcttccgc cgaaatcgtg caggtcgaat ataccagcgt cccgccggag cgaagggctt
     6301 tgagtccgct cacgactaat tgttcttgta taaacgcaaa tttattcaga tgacgcaaat
     6361 tgctgacggc ggatggcgct ttatgaatta cccccagcgc cgaacacggc gcatccacca
     6421 atacttttgt aaacgatccg ggtaccagat ttccgatgcg ctcgccctgc atattgacca
     6481 tggcggtatt gatcacgccg aggcgatcca gattatgtga aagggttttg atgcgtttgt
     6541 cgtcgcgatc attggcgacc atacagccgc catttttcat catctgcgcc atttgtgtgg
     6601 ttttggaacc gggtgcagcc gacacgtcca aaacaaaatc cgacgccgtg ggatttaaca
     6661 ctatcggtgg caacatcgaa gcgatacttt gtatataaaa taagccgagg aagtgttcaa
     6721 tcgttttact gatccgcgca tcttcgcctt ccaccagaag tgcatccggc atggacggga
     6781 tttccgtaaa ccgaaaattt tgttgcgcaa gccgatggat gaaatccgtt ttgttgaact
     6841 tcagcgtatt cacacgaagc gccgtacgcc gctcaaaatc caggccttgc gaaaacgcgt
     6901 ccgcgtattc gccgggaaat aatttaagat attcttttaa cgcttccggt atttcgatcg
     6961 gcataagtaa agtgtgttaa actgtatgtt gcaatcgggc taaataacaa ccttttggca
     7021 atagtgcaac gacgaattgt aaaatcgcgt cattataacg aataactctt cttcatttcc
     7081 ctttgcgact ttgcgagaat gaatgcggct tcactacggc gcgtttggtt cgatcatgcc
     7141 tatggcgtga atcaccatga aataccttga cttggaaatt atgaaacgta ttgtaacaca
     7201 ataaacaagt agaacgacga ctgcgttgtc tcacctgcgc aagcgggtgt ctatacaatt
     7261 tgtggcccgc aagaacaaat acatgctcgc taagccgctg agacgccaag gcataaaatc
     7321 gtaaaaacct tttccttttc cctttgcgac tttgcgagaa tgaatgcgga tttgctgagg
     7381 ctaaaaaaac ggcatcaacg atctcataat ttactcaaac tttaataacc acgaggaatt
     7441 cgagaacact tttgaagtga ccgggccatt acgagatatt tacataacat aatctttgcc
     7501 tttgatcgta cggcatcctt agattgcgcg caatctacaa aggatacgca tgtttgctga
     7561 agttgttttt gacctcgcga tcgaaacacg ttttacgtat ggcattcccg acgcatggga
     7621 atccgtcatt gttcccggtt cgcgtgtact ggcgccgttt ggccgcaaaa aaatcaccgg
     7681 ctatgtcgta gcattatccc gccacagcga acgcgatcag atatctccga tacacgaagc
     7741 attggaccct cttccggctt tctccgaaga tcttttgaag ttagcggaat ggatatccga
     7801 ctattatatg gcgccgctcg gccaggtgct cggcgctatg ctcccgccgg gcatggagcg
     7861 tcacggtgaa aaactgtatg tgctcaaagc ggtgattacc gattttgaaa tcaaaagctt
     7921 atctaaaacc aaacccgtac aatccaaaat tctcaaagcg ctttacgttt ataaaaaact
     7981 cacgttgcgc caattggtca aaaaagtcgg tgcgcgcaat ttgataaaaa atttggacga
     8041 actgttgcgc tcggctgtcg tcgaagagcg ggatgtcatc agcgatcaat tggctaatat
     8101 acgaacggat acctgggttc gtctctcgtc gcttctgctt ggcgacgaag agcgttttgt
     8161 atgggctaca gacgaactcc aaaaacgcgc tcccaaacaa gccgatattt tattttacct
     8221 ggatgaagcg acgcgccagt cggaagatga agagcctttt gccctacgcc aggccgaact
     8281 tctgaaaatc accgaggcgc cgtattcggc gctcatcgca ttggagaaaa aaaactggat
     8341 cgaacggatc gaacgcgaag tgatccgcga cccgtatcaa cgcgactacc ccagacccaa
     8401 cgacgtcaca ctcaacgatc atcaggcgca agcggtcgct gcgatcgccg aaacattgcg
     8461 taataacaaa tactacacat ggctgctcta cggtgtgacg ggcagcggta aaacgcaggt
     8521 ttatatcgag agcattagac aaacgctggc gctcgggaaa aacgcgatcg tactggtacc
     8581 cgaaatcgca ctgacaccgc aagccgtaga acgtttcaaa gcgcatttcg gcgatcaggt
     8641 cgcggtactc cacagccgta tgacgatggg cgaacgattt gacagttggc gaaaactgca
     8701 aagcggacat tttcgtatcg ccatcggcgc acgttcggcc gtatttgcac cggttcagaa
     8761 tctcggattg atcgtggttg atgaagagca cgaacacacc tacaaacagc aggataatat
     8821 cccgccgtat catgcccgcg atgcggcgat catgcgggct tcgttttgca aagcgaccgt
     8881 gatcctcggt tccgccacgc ccagcatcga atcctactat aacgccacga cacagaaata
     8941 cggcttactc aagttgccca ttcgtatcaa cgacgtccca atgccggaag tggaagtcgt
     9001 caacatgcgg gaagaatacg aaaaacacac gtcggattgg cagccgattc tctcccgtgc
     9061 attacgccaa cgcatgaagg aagaattgca gcatcgtcgt caggcgattc tttttcaaaa
     9121 ccggcgcggc tattcgagtt ccgtcgaatg ttttgactgc ggtcatgtcg ccgaatgccc
     9181 acggtgcagt atcgcgttga cgtatcacat caccgggcac aaactgcgct gccactattg
     9241 cggttttcat caaccaatgt attcggagtg tccgaagtgc gatagtctta gcatcgccca
     9301 gcaaggcatc ggcacgcaaa aagttgagga acaattgcaa atggcatttc ccaatgccgg
     9361 tgtgttgcgg atggacgtgg acagtacgtc cgccaaaggt gcgcatcatc gcattttgga
     9421 ggaattcaaa aataagaatg cgctgatttt attgggcaca cagatggtca ccaaagggtt
     9481 ggactttgaa aacgtcaccg tcgtcggtgt tatttcggcg gatacgagtt tgttgctgcc
     9541 ggattttcgc gccggcgaac gtacttttca acttctcacc caagtcgcgg gccgcgcagg
     9601 gcgaaaaaat aaaatcggac atgtcatcat tcaaaccatg caccccgacc gcgcggcgat
     9661 ccgtcacgcc cggcagcatg atttcacttc gtttttcaat gaggaaatct ccatccgtca
     9721 tgatttgtta tatccgccgt tcggccgatt aatgttgatt cagatccgtt cggaggacga
     9781 acaaaaagct gttgaactat cgagtgtctt tagcgatatt cttgcaggcg aaatcaaacg
     9841 ctatcccgat ctggcttccg gcgtggctca attaggcccg acaccggcgc ccatcgtgaa
     9901 aattcgaaat tattttcgtt atcacatttt attgaaagtg gacaaagaag cggatcggtc
     9961 cggttcccgt atccgttcta ttctccggca tacgcgcaaa caagttattt cccgaattcc
    10021 atccggagcc gaaattcgca ttgatgcgga tccgctttcg ttactttaat ccgataaagt
    10081 tatgagcaaa cgatttgaag aaaaacgcga agcctttgag atactcaaca aggaactcag
    10141 tgatctcata ccgcttgagc aacgcgttca aaaaaaggat aaatcgcctc atgtggtaga
    10201 tccgcacgcg cacttgccca acgatcccgc caaaagcgat gcgaccggcg ccgtacccaa
    10261 agagtggctg gacgaacaag ccgcgttgca cgaaaaatat aaattagtcg agcgcgttta
    10321 taagttttac cgcaatatca tacagaatat gagttcgagc attatctgta tggatatggc
    10381 cggtcacatc accttcctca atgccaatgc cgccaaaaca ctggaatatc aggtcgaaga
    10441 attgctcggt catccgatga cggacatcgt cgcggaaccc ggccgtgaat ccaaaatcat
    10501 tgatcgtatg ctcatcggta acaaacgttt tgaaagtaaa gaggtcggtc tgattgccaa
    10561 gtccggcgcc gttttaccga tcggattcag caccgcgccg ctcatcgaaa atcacgaaca
    10621 aatcggcgtg attattacgt ttcgtgatct gacagaaatg aatctgatgc gcaaacaagt
    10681 cgaacgaatg gatcgtctgg cgactttggg tgagttggcc accggtattg cgcatgaggt
    10741 gcgcaacccg ctcggcggca tcaaaacagc ggctcaggtg ctcgaagagt ccttcgaaga
    10801 caacgaccgc cgattagaat tggtaagtcg tatcgtgcgt gagattgatc gcgtcaacca
    10861 cttactgacg gattttttca aatttgccaa acccgtcaaa ccgacccgcg atcactttga
    10921 tgtcgaatcg ttgatcgacg gcgtttatct tttacttgcg gcgcaaatga attctaaaaa
    10981 gattcgattt aaggaagatt tttcctcgac attacccaaa gtgtacgccg atccgcatca
    11041 gactgaacaa gtattgatga acatcatttt gaatgccgtc caggccatgc cgcaaggggg
    11101 agatatcatc gtacgtacgt acacgacgac cgtcggtgaa agtaaaagca tgattcatca
    11161 tacggatgaa ttttttgata aaaacattcc gtttgtggcg atcgagatca gcgatacggg
    11221 agtcgggatt ccgcaggaaa atatcgagaa aatattcaat cccttttata ccaccaaacc
    11281 cgacggcatg ggcctcgggt tgtctatttg caatcgttta ctcaccgaaa ataacggaaa
    11341 tctgcacgtg gaaagcgccg tcggcaaagg caccaagttt gtcatcatgc tgccgacgcg
    11401 ttaaaacgca gcatcggtat gtgcgatttt cgcatcctat taaaatccgc tacctctctg
    11461 taaaacacga tcagatcatt gcgtatcggg aggtcactcg gcacacgcaa tcccgcattc
    11521 aatttgacca accctttttt catctgcaat tcataaccga ccgcattatt cctgtccaga
    11581 tgatacagcg ctacggcgat atggagcacg ccttgcaata atcgcttaag cgtttcctca
    11641 ttcgtatcgc gccaaagtgc ctcgagcgtt tcatggcaga tgtagaattg ttttcggtta
    11701 aactcttcta ccgcacggag ccataacgca tgctgcttga gggttagcgg gttcaaacgg
    11761 tggccggcat atcgtgaata tacgcattca tcattttgag ttcggcgtct ttttcttcca
    11821 gattggtttc aagcaaatcg cgcacggaga tcataccgag aaatttttcg ccctccacga
    11881 ccggcaaatg ccgcgagtta acgcgtttca tacgttccat gcatacatcg tacgattcac
    11941 cggcggaagc gacggcgacg tttttcgtca tcaccgtctc gatggtcagc gcgcgcggat
    12001 ccagcccttg caacacaacg cgtttgagca aatcacgttc ggaaaacaca ccccgaagcc
    12061 gcatcgtggc ggagctgtca acgatacaaa tcgcgccgat gtttttggaa gccatcagtt
    12121 ggatcacatc aaacacattg gtacctaatg tcacagaata cggttcgcgc ccggagataa
    12181 tagcacttac ggttttcacg gcaacctcct tggttggttg caaaagacgg ttgtttaaga
    12241 ataatgagaa agaaaggcgg gtaaaatata aacgcaaaag ctgatgatgt caataagcgc
    12301 gcatcaatct tccgtacggg gaaactccat agccagaagg ataaacgata tgccccaaag
    12361 ccccatacct tgagcatacg gcgcgccaaa agcgagaata ctgcaaccga gtaacccgat
    12421 catttccgca acggcaaaaa gtagaatcga acgtttgatc cgttcctgtt cggccgcttt
    12481 cggatcatgc gaaagttccg cgacggttat gtctcttttt tgccgaagaa aaacaaaagc
    12541 cacggcggcg ccggccgcga tgaacataaa aacataatgt acgggaaaag gcaatgagga
    12601 caaactctca ttttcctttt cgcccaaagt cggaagaatc accaggtgca ctaccgccac
    12661 aaaaacaaac aacgaaaata tcactgcacc ccaaatgata cgaagaatag ggagtttggt
    12721 gttgggtggg ctgatttcac tcatggttat ctccgttaca acgttttgtt gttttgatgc
    12781 cgaattatat acccctccgc ttgtagcatg gtagtgcatt gcttttgatg ttgttttatg
    12841 ttttcaaact ttcccgagat tgatctttcc agcaatccga gtatattaaa aaaataaata
    12901 gcctgtcata gtcgacaagt tgtaacatca gctttcgtta gtcttacggc ttttatttta
    12961 cgggtttgtc tatgcgcgct gattgtattt ttttaatctt gtttagcaaa aaaagtttta
    13021 actgcatacc catccatggt tttttaatta ccctgtgggc ttttcataat acttattgat
    13081 tgaggttcag tacttgcaaa agcggataat ataaaacttt agcaaacgtc taaaaaatac
    13141 ttgttcttaa gcttgtgtta aagtccgata tcaattctca attgctttaa atttaattcg
    13201 gcagagtaca actcgccaac taaatcgttt gctataaatt tatatctatc ttgatattcc
    13261 gtaaatgagt taatgacctg agagtatttc tttgagtcat ttgcaattac atataatcca
    13321 acatttgaat aatccattaa tgttgccatt ctgccaacac cgctccaaac ccccgtcgac
    13381 aactcaactt caaaagcata ttcaggaata taattattac ctctagtttt aaaccaaatg
    13441 acatcaattt gtcttaataa atcatagtct gaaaattgta actcgggaca agtcttaagc
    13501 gttgaaactt cttcaagatt cttgtcgtta aattttctgg atttatctgg gcaaaatgtc
    13561 tggtaccccc ttatatttcc gatttctaat aagcggcctt gtaataccga atgcaaactt
    13621 tcctcaacag ttttaggctt ctcattttca tcaaactttg gtggtgatag aagtagatcc
    13681 caattattga tgaaagtctt acattcaagc ctattagacc tccccgataa tactatattc
    13741 aggtcttggt caatgttgat tttatattct ccatttctat cgtgactaat ttggtgaagg
    13801 tgattgaaaa gtaattttgc aggaataata actattcttt ctattgagcc acaaataaaa
    13861 gcaatgaatg ggttttctag attagctatt tcttcaactg ttatatagtt aattccaaaa
    13921 aaatatcgct tattaccatc tgaagatgcc cttactaaaa cattcgcgtc tccaatcttg
    13981 tacgtccttg atttcaaaac tttaattaat tcaacatttg cattaaactg ctttgccatt
    14041 cttaaaaatg aattcgccaa taagtctgat ggagaaatct ttctacccat tattgctttt
    14101 atccattatt tctaagaaaa caccgtaata tatgtatctt aaattttcac ctgcaccaaa
    14161 ctcaagaagt tttttcgctt ttgtatattc ttcttcgttc atttccctac ctaaataatg
    14221 aagcgcttct tcacgcaaat cttcttttgt aattctaaat agaacttcgt tatctgcttc
    14281 cataaaaatt tcagtgattt tgtgattttg aatcaaacac taaccaaatc gtacttcgta
    14341 agcttactat tcgatataag cgaattaaac aatgaaacgt ctataccgac agcatgcatg
    14401 ccttgctccg agctttgaac aagtgttgtc ccactaccgc aaaacggatc aagaatcaca
    14461 tcgccttcct tgaagtaagt tcgctttttg aaattatccg tatgtttgtc aataaagtat
    14521 tctacaagtt gcggtataaa ttttccttta tagggatgca gcctgtgaac gtgtttagtc
    14581 gtttctgatt ctttgtattg atcaaatgat aatgcccaat ttagatcgga acccaaatga
    14641 tttttccatg aattttcgcg attccgttta aattcactat agtagcttat aagttcttcc
    14701 cgagaaataa gcgtggatcc attttcaccg atttttttta tacgcccata ttgaatcaaa
    14761 taagcgatat tagatgtaga tacatccttt cctgtatact ctgtagccca atgactagct
    14821 tcttttattg taaacaattt atctttcata catgcccgtt tatttgctac taccttacct
    14881 tatattcccg ttgctcggtg cgtttctgat ctttttttgc tatagcttcg cgtttatcgt
    14941 acagtttttt tccgcgtgct acggcaagct ggactttgat acggttacgc tcgttgaaat
    15001 aaaccgacag cggcaccagc gtcatgcctt tttcctggac ttgccgttcg agtttacgaa
    15061 tctgcacccg atgcaaaagc agtttacgcg gacggtattc ttcatgatta ttgtagttag
    15121 cttgtttgta ttcggggatg tgcatgtgat ataatatgac ttcaccgttt tcgatgcgcg
    15181 catggcagtc ctgaatattg attttacctt cccgtaaaac tttgacctca ctgccggtca
    15241 aagcaatacc cgcttctaag gtatcaataa tttcaaattc aaatcgcgct ttgcgattgg
    15301 tcgcaataga tttgtattcg tatttctttt tttccgttgc cgtcgccata gattattcga
    15361 tccgttcttt gacgggcact tccttctgac ggttaaaatt atgaacgagc atttcgccga
    15421 tcagaccggt ggatatgatc tgaaatccga tcataatcag aagaatgccc aaaaacaaaa
    15481 tcggacgttg gcctatgggc gtacccaaaa accaatcgaa cgacaaataa ccgcaaatac
    15541 cgagcccggc caaaagtgac agcgatccaa aaaaaccaaa aaaatgcaaa ggacgtttga
    15601 cgtaacgcgt caaaaatacg accgtgatca gatcgagaaa tcctttgaga aaacgtgata
    15661 atccgaactt actttggcca tatttgcgcg gatgatgatg cacgacgatt tccgtgatgc
    15721 gacggtaacc ttcccatttt gtcaaaaccg gtatgtagcg gtgcaattcg ccgtacaaat
    15781 tgacatcttt gaccacttcg cgccgataag ccttgagacc acagttgaaa tcatgtagac
    15841 gaatcccgct catcacacgg gtcacatagt taaaaaaacg cgacggaatg gttttggaaa
    15901 tcggatcgtg acgcaccttt ttccaaccgg aaaccaaatc gtttccctcc tgaagtttgt
    15961 taaccagatg aggtatttct tccggatcat cctgcagatc ggcatccatc gtgatcacat
    16021 acgtacccgt cgccattttg aacccctcgg acaaagccgt agattttccg aagttttttc
    16081 taaatcgtaa tgctttgacg gcggcgtact gttgtttgag tttttgaatc acggtatacg
    16141 aattatccgt gctgccgtca tcgacaaaaa tgatttcata caccacaccc atgcgggacg
    16201 cgacggtatg aattttttcc gtgagttcgg gcagagattc ttcttcatta aatagcggta
    16261 tgacaacgct aatggaataa ttgggatcgg tcacgcgggt atcctgttat tgcatctcca
    16321 cgatatctat atcgcgggga tatttgattt taaatatact gctgttaaaa gattcattga
    16381 ccgtacgcat atcaaaaaac aaactcaaaa ggtcttgttc acgcggccgc acgagacgaa
    16441 cgtacttagg aaaatatcgg ccgtcaattt tctgaaaccg cgaaaattcg cgctcgacgg
    16501 atacatcgcc ggtcgagtcg taatccacga tgcggctgac ggcaaacccg gcatacggat
    16561 caaaccatat tttacgaaga ccttcgccgg tagcaaactg cattacaaaa taagtgtcgt
    16621 ccaccgaaaa ttcgcgcaga cgcaccagtt cggattccgg cacgcgcggc aacccgacca
    16681 aaagctcaac aagttcgtca taattgacat tgtaatcaaa cgtgcgccga atagccgacg
    16741 cgctggtttt gccgataatg acttgtttgt tgataatatt atacaccaca aacgattcgc
    16801 gattgagcga cgccttgaca ccgtcaatac cgagcatgcc ttcgattttg atatacaccg
    16861 aatccggtaa acgaatagca atctgcgccg taaattgtcc gccgctcttg ggcgattcga
    16921 cattcatcgt agccaccgat ttgatatgac gaatcttagc ggcattttca ttgcaccggc
    16981 gaagaacttc caacggagtg acggtcgtaa tatccgcagg ttggcggatt acttctttgg
    17041 gtgcgccgca tccgctaaat aggattaaag ccataaaacc aaagaaagat aaaaaaaacg
    17101 aacgatgata tttagtgtga tagtgcattt attttttccg tgagcataga attatcagga
    17161 ttgatcgtaa gcgctttttg aaaagccgat aacgcatctt gggtattgcc caatttatga
    17221 tgaatttcgc ccagatgcgt ccatacttct tcactgttgg gatgaactgc aacagcgcgc
    17281 tgcgtataga taagcgcttg ggcatattct tctagttgaa acaaaaccca acccatcgtg
    17341 tccaaataat gaccgttgtc gggcgctttt ttgagcgctt tttcgatcat ctgccgcgct
    17401 ttgtccaacc ggattctgcg ttcagccaaa ctataactgt aattattaag tatcacttcg
    17461 ttgttcggtt caaaaacaag aatgcgttcg aatacgctat ccgattgggt gaaccgtttt
    17521 tgattctgat aaatcggcgc cagaagattc atcgtcagta aatcgcggcc attgagcatg
    17581 gatgcgcgct ccagcacgtc caacgcttcg acggagcgtt tgagctgcac caaggcaaaa
    17641 cctaacgagc gaagaaaatc aaaattattg ggctgtagcc tgtccgcatg gcgaaatgcg
    17701 cgtacggctt tttcataatt accgctctga atagacagca gtccgacact ggcatgcacc
    17761 tggagattgt cttcttgaag ttcggcaagc gattcgtaaa tttcgatcgc tttaggaatt
    17821 tctttattaa aacggtacgc catggccagc gccgatagta tttccgtatt attgggatcg
    17881 ctactgcgtg ctctgtgata tacatccacg gccttatcat acgcacggga gcgcatgtag
    17941 atttctccca gtttcaatga aagcagcgta ttcgcgccga taacgtccag caaccgtgcg
    18001 taatgtaccg ccgcttcttt ggctttgcct tgcacttcgt atatcgtgat gagccggtaa
    18061 tgcgcatcaa tattcgacgg atacctccgg ctcagcaatt ctaatgtttg aatggcttta
    18121 tcaaaattat gctgtcggat atgaatatcc gccaaaactt ccaatgcatc cgggttaaaa
    18181 ggatctttga tcagcacgcg ctgaacgagc gccagcgctt catcgaaacg accgagttct
    18241 agataatctt ccgccaacgc gttggacacg ggcaatgcat tgctgtcgct gagaagagca
    18301 aaacggtagt gcatttcggc ttcgccaaat tcgccggaca tatcggcaat caatgcatcc
    18361 accaaatgct gaacagccgc cggtttgtat gcatcgtaac gcgaatcctg tgtaccgatc
    18421 tgcgaatgca tacgcgaacc acaactccaa aggagcggca aaatcaaaag aaagacggat
    18481 gccttataaa tacggtttcg cataaaaaca gatcgtgtta taaacaaata cattggtcgg
    18541 aattttttca ggctacaatc agatcgtccg gcacttcgat ttccatttca agtaaaccca
    18601 taccgtgggt tttgccctga gcatccgttt tgaggctttc cgtacctccg ccgccgagcg
    18661 aatcatgaat gatgaaattg agcgctaaaa gattgggcac ttcataacga tccaccggac
    18721 cattggcgat tttagaaaaa tgccgacgta cggcctcagg tgtcgcttgt gcacacaaga
    18781 tagagtaata ttcttgtttg taagcgataa gtccgacatt ggaactgtca cctttatcgc
    18841 cgctgcgcgc atgcgcaatt tttttgagct gtattttttt caaacggctt cctcgtcctc
    18901 ttcttcgagc gtatagttac catctttgtc tttgatcaga attttgagtt tttgttccgc
    18961 cgtattgagc ttagcctggc aaaactctac caatttcatc ccgcgctcaa acttaaccaa
    19021 cgtttcatcc agaggcacat caccgtgctc caatgcgtcc acgatcgttt caagttcttt
    19081 gagcgaggcc tcaaacgtgg tctcggatga ttcttcgttt tgtttctttt tactcatagg
    19141 tataacctta caaatcggca ttataataat cattcccatc atcgtttgca acgatgttaa
    19201 atgaataacg ggacacaatg gctcctcata agcctccgat ttttaaatta aaaaagtaat
    19261 tcattttact aattttttat tgttgtgctg aacggtctta tgtatcttgg cgctggtaac
    19321 cgtttttttt aatggaatcg aaaccgcagg agaacgtatg gtacttcgga tagctctatg
    19381 gttgtgtgtg gcatggccgg cgatggcgca aattaaggtc gtggtattcc cgtttcacaa
    19441 taccacacag gcacaaaaaa atgattggct ttcgcacggt tttgccgaag cgctcaatac
    19501 ggaattagcc aaagacacgg cgctggatgt gattgaccga accgatgtat tggcagaaat
    19561 caaatccaaa ggatggaaac aagccgacat caaagatgaa gctaaagcta tggctgtggc
    19621 ccgttcattg gaagccaatc gcatggtgta tggcgtattt gacgccaaag attcttcgat
    19681 caaagtattg accaagtttt ttgatcttcg caccggcgtg gccgacggaa aaaacacata
    19741 tatcggcgaa ggcaaattta cgacgaataa tgtgcatgcc atgtacggcc agattgccaa
    19801 taaagccctt gtgagtttcg gccgtaaaac catcgatgaa atcggcggcc cgacaaaagg
    19861 cgccattaat gtggacgcct acgaacccta tatcaaaggt attctcaagt acgatgaaag
    19921 cagtacggta gatgattaca acaaagccat cgaaatgctg acgcaagccg cgaccattga
    19981 tacgggattt gcactcgctt atgcgggcat tgccaaagcc tacgctaaaa tcgcacgcat
    20041 tcaggacgtc agcggtaaat tgaccgaaaa ggaagaaagt tataataaag ccctcgaagc
    20101 cggattgcgc gctgtgcgca ttgataaacg ccttgccgta ggttggacag cgctggcgct
    20161 cacgtaccgc gaactgaaag atcgcgataa actcatagaa gcggcacgta aagccgttca
    20221 aatccgcgtt tcgcaatacg atgcctacga tatgctcgcg gatgcgtttt cgccgagctt
    20281 tttccctcaa tacaaaaata tcgattcgtc cattttttat cgcaaaaaaa gcgtgacgta
    20341 caatacgaaa tttgcctccg gattccgcgg attaggcggc gactacatgg ataaaggcga
    20401 ttataaaaat gccgagcagg cgttccaaaa agccgtcagt atcaacccca aacacgcggg
    20461 ttcacgcgat cgcttaggtc aaatctattt cgcgcagggc gattacatca aggccaaaga
    20521 taatttttcc gaagcgattc gcttagacgc caaaacgcct ttcggatata cgcatcttgc
    20581 cgatgtgctc tatatggaag gaaaatatgc cgaagcgata gccgcttatg attcggctct
    20641 gcatcataat cccaagtttg cgatggcgca ttacggtttg gcatggacgt tactcagttc
    20701 caaacaacga tcgttaaggg attccgtccg ggcgctgacg cacgcgactc aagctgtgga
    20761 aatcagcgga caaaaaaatg ccgccttcct atctgtgctg gccgaagctc aatttgctaa
    20821 cggcaacaaa gagaaagcca tcgaaacgat tactttggct cgtgcgttgg attcggccaa
    20881 tacggactat gaattgtccg aaacacgtta tgctggaaaa gataaatccg gcgattatgc
    20941 ctacgcgatg cgtcgcggcc agatgttttg gaaacaaggg cgtggagcgg acggcgtaaa
    21001 agagtttgaa gaagctaata aattaaatcc taaaaatgtt cacgtgctgt tgggattggc
    21061 tcgttattat gatcaggaca aacaatacaa aaaagcgttt gaccactatt ttctcgcacg
    21121 cgcctgcgat tgggataaaa aatacgtcaa acaaattcag gaacgaatga acgcgttgga
    21181 aaagcattcc aaataaaact tcagttcacg tctgtaaaaa aacctcaaac aaaattgttt
    21241 gaggtttttt atttcacatc caaaacgcat tttcgatatg ccggatttct tcgccgtcgc
    21301 ttcggaggcg caccgtgatc agatcaaatc gacaatcttt ttcaggtgcg ggatatttgg
    21361 ccaagtaata ttcggcggct tgttttaaaa atttttgctt tttggacgta agccatgtct
    21421 ccggctcacc gaaagctgat tcgtcatgcc gccgaagatc ggtggttttg acttccacga
    21481 tgacgataaa agtttttgat tccgcgacaa tatccagctc accattgcga aaccgaaaat
    21541 gccgatgcct tatatcgtat cccttctgtt ttaaaaaaat ttcagccaga ttttcgccgt
    21601 gttttccggt gtctgtagca tgactcatga tgggatgccg aaaattcttc tgtgaattgc
    21661 ccgtacgaac ataaaaacag aaaggtctgt gccgattacc gtttcggata aactcgcttt
    21721 acgtggattc gtacgataaa aataaagaac tatgtgttaa aataaatttt ttcgcagtaa
    21781 aactttacaa cccccccagc gctttcacga tacgatcttc gaactggcgc atggtttcat
    21841 aatattcctt cgggtaaccg ttggtcaaaa tactaaacgc aatgatgtct ttggattgcg
    21901 tttcgatata cccgctgagt ccgctgacgc catagagaaa acccactttg agacgtgtcg
    21961 tgccgatcaa cgaagactca tctttgcgtt tgataaatcg ttttttttct gtgccatcaa
    22021 taccgccgat actcagcgac gcgatgtaat ccgaagcgta gcgaaaatca cgatacatat
    22081 attccagtaa tcgcacctga gcccgcgtcg tcattttgtt gcgttcatcc aggccgcttc
    22141 cgtcggccat ctgaatctct ttttcggaaa ttcctacttt ccgtaaaaaa ggctgtaaag
    22201 cacgaattcc tttttcgtct gttccgggtt caccaaatgc ttcggcgccc atcatcatca
    22261 ggagttgtcc caccacataa ttattactcc acttattgat gccggagaca atgtaagaca
    22321 aatcatacga cggaggatct attaaaattt tggacgcttt cggtgtttta cccgtacgca
    22381 atcctccact gaccttgata ttcataatcc ccaaggtctc tttaatattg gcgatcgtcc
    22441 accacgtcgg attgggtacc cgcttgtata tgacaaattc gttctcattt ttggcaatgg
    22501 tcccgtacaa ggcaaccgtc atctgatttc cattgtatcg tataaaggta ttattgtgat
    22561 tgacaccgat accttcgccg gaatcggtcg tcttaacgcc gttgataact ttgacaagag
    22621 aggagatcgg ctcgataaaa acaatcgccg agtcgccggt tttttctccc ggacgaacac
    22681 agatattgat gatattaaaa ttgaaaccca aagcgccgcc aacggccgaa taggcgcgat
    22741 cgttcgtttc ggcattttct gtatcaatca aatacgtatc gtcacccacg atgtcgccgc
    22801 gaatttccgt caaccccatc gctttgaggt gatagatcgc acgcaaaaga atttccggta
    22861 aaaaatacgg atcgccgaga cctctgatat agagatcacc gtttagaacg ccattcttaa
    22921 tagccccgtc gtaacgaaat tccgtgcgaa aacttttatt ggttccccaa cgatccagag
    22981 ccgccgctgt ggtgacgagt ttcatgcacg aagccgtgga acgtaacgaa tcgggatttt
    23041 tttgatgaat gacttgaccg gtcagaagtg acttggcaaa aaaaccggtg cggatgtctt
    23101 taaaaaaatc atccgtatat acggcatcaa tagcgcgccg tacactatct aattgcgtgg
    23161 agcgatactg tgaaaataac gaagccgccg taaaaaaaag aatacatccg tagcgccaca
    23221 tcatgaacta tcaatccagt tgaaatggcg actgaggatc atgttcgtgg cgaatttcgt
    23281 ccacgggttc ttttttccct ttgccggcgt acagtgtgtt ggcaaaatta tgaacgatcc
    23341 ccggtttgcc gccgacattt ttgtaagcat ggacaacccc gggaggaatg atcacacttt
    23401 gcagattatc ttcaccgaca aaaacaacca tcttatgacc gtacgtcggc gaatctttgc
    23461 ggttatccca catatagagt ttaaaattgg aaggccctat aaaacaaaac agatcggttt
    23521 gttggacgtg ttcatgcgga ccgcgcgcca cacccggcaa tgtcagcgac gtataaccca
    23581 tgacgggtac attttcagag gccaattcgt cttgacggta aagctcagtc agccatccgc
    23641 gatcatcaac aaacttttta tggtttttaa gaatcacacc ctgtatgggg ccttttttga
    23701 aagtcatgaa acgttacttc tttttgttgg ttgtttcgga tttggataac acctgatata
    23761 cacgttcgcc ttcgcggatc atagtatatt tttcacgggc gattttttcg ataaattcga
    23821 gatccgtttt caaacgatcc cgctgatcgg caagcacggc atgttcgcgt ttgagctttt
    23881 cgatttcttt ttcgacggca gcacgctgct gtttgatttt ataaatctgg tatgcgccgt
    23941 aattgccgga aataattgtc agtgcaccga taccgatcaa agaccaaaaa agaatgcgac
    24001 gattacgttt ctttttgatt tctgccggat cggtcgattc gaccacggtt tctttcgttt
    24061 cgtttttcat atttcgtatt cctacacttt gaatgcttta cgtcccggga aatgagcacg
    24121 gccgccgagt gcttcgtcaa tgcgcagaag ttgattgtac ttggcaatac gatccgtgcg
    24181 ggaggccgat ccggttttga tttgaccgca attaagcgca accgctatat ccgcaatggt
    24241 cgtatcttca ctttcgccgg agcggtgact catcacggcc gtataaccgg ctttgtgcgc
    24301 catctgtacg gcatctatcg tttccgtcaa cgaaccgatc tgattgactt tgacgaggat
    24361 ggaattggca acgccttttt caataccttt ggccaaaaat ttaggattgg tcacaaaaag
    24421 atcatcaccg acgagttgga tttttttacc gacagcatcg gtaaggtgtt tccaaccttc
    24481 ccagtcattt tccgccatac cgtcttcgat cgagatgatg ggaaactggc gaacccaatt
    24541 ttcccaatac gccgccattt gctcggatga caacttacgt ccgtccgatt ttttgaaaac
    24601 atattttccg ttttgataaa attcgctgga agccggatca agagcgatca taatatcttt
    24661 accggcttta tatcccgccg cttgtatcgc ctgcaagatc acttcgatcg cttcgtcatt
    24721 ggatttgaga ttgggagcaa atccgccttc atcaccgacg gccgtattgt atttttttcc
    24781 tttgagtaca gtcttgaggt gatgaaaaac ttccgcgccc atacgcaatg cttccgtaaa
    24841 tgaacgcgca ccgacgggca tcaccataaa ttcttgaaag tccacattgt tatccgcgtg
    24901 ggcgccgccg ttgatgatat tcatcatcgg cacgggcaac gtattggctt gaataccgcc
    24961 gatgtattgg aacaggggga tattgacttc attggccata gcacgcgcag ccgcgaggct
    25021 gacgccgaga atggcattgg cgccaagttt gcctttgtta gctgtgccgt caagtgcgat
    25081 catcgtatga tccaatgtaa attgatcacc ggcttccatg tgcatgattt ttttggcaat
    25141 gatcgtgttg acattgtgta cggctttttg aacacctttt ccaagataac gttttttatc
    25201 gccgtcgcgc aattccaccg cttcatgttc tccggtagat gcaccgctgg gaacaatggc
    25261 gcgacccata gcgccgcttt ccaagtgaat ttcgacttcg accgtaggat tgccgcgtga
    25321 atccaatact tcgcgggcat gaacgtgatg aatgtgactc ataactattg ctcccgatgt
    25381 tgaaacgccg gtggcgtgaa attattattt attgcgttgt atgttatact gaacaaattt
    25441 tttgagcgct tgacgcgacg gggaatccgg aaaatagttg agcgtttgta tcgcgcgatc
    25501 ggaatattcc tgtaaccgtt ttttggcgta ttcgatgccg ccgtagtgtt cggtaaaggc
    25561 aacgatctgc ttgatatcgc cggacgtcac gctttttttg atttttttca aaaccgcttt
    25621 gctttcactt ttcggcgcac ggctaaaagc gtaaatcaaa ggtaatgtga ttttttgttc
    25681 tttaagatca cggcctttgg ttttgccgat gatggtttgt tctccttcaa aatcaaacaa
    25741 atcgtctttg atctgaaagg cgacaccgat caattcgcca tattcgttca acgctttgcg
    25801 atcttccggc ttttccgttg tcgtgatggc gccgagttcg caggccgcgg cgatgagagc
    25861 gcccgtttta tcggatatca tccgcaagta tgtcggttcg tcaatatcca acttcctgct
    25921 cttgtctatc tgcataagtt cgccttcgct ggcccttttg gtagccacag acaaaagcat
    25981 catcgcgtca aaacttttga tatccacgat actgatgagc gcattaccca aaaggtaatc
    26041 ccccatcaag acggccattt tatttttcca aatagcattg atcgccggca aaccgcgccg
    26101 catatccgcg ccgtccacca catcgtcatg taccagcgta gcggtatgca ccagttcgat
    26161 ggtgatcgcc gcacggtagc tgcgttccgt tacggaacca ccgcacattt tgctgcacag
    26221 catgaccaaa accggacgca ggttttttcc ccgggaggaa acgacataac gcgcgatcgt
    26281 gtctacgatc tttattttgg aagtcagctg tgtgctgaat ttggttttaa aaatttcaag
    26341 ctcatcttcg atcggatcga agacatcgtc taaagtcatg ccgaagtccg taaaactaaa
    26401 tctttataaa cttgggacaa tgtaggcgat tgcaatatgc aaatcaagat gcgaactgat
    26461 atccgatgag ttgttattaa acggcatatt aatgacctcc ttccgtttct ctttgcaact
    26521 gagcgaaaga gaatacgatc tcgctaaaac ctcataattg aatgcgaagt gctttcgctt
    26581 gaagcgttgg cgttaactta agaattgacc ggtataaggt catcaataca atcaaacgtt
    26641 tttgttcctc tactctttgc gactctgcga gagagcatat gaactcgtta tgggctaagt
    26701 acgcagaaaa atatcaatgt ttttgtttcc attcggggaa acgatattac acataaccga
    26761 tcttggacac gatctcataa ccttttttta ttttctttgt cttccatccg ctttttttta
    26821 catttggtgc ggttgtaatt cattttgttt acataaggtt ttcacaccgt attttacggt
    26881 tttatttatg ccggaacagc cttcaacaca gcgccgattc gaagaacttt gggcaggtcg
    26941 cggttactcg acgcgcatcc aaaattttca ggaactcatg cgctttaaag tgcgtgatat
    27001 tctgttgatt tccagcttgt acgattccta catacttgaa gaagacgggc gtttattcga
    27061 actcatccag gaggaatacc gcgggctcga tatgaatcaa gcgccggata ttattcacgt
    27121 atccaatggt acggaagcga tacgcatgct ggatcgccag caccgttttg acctcatcct
    27181 gatgactttg cacatcgagg atatccacgc catcgctctt gccaaaaaaa ttcgttcgct
    27241 tggccatgaa atgccgatca tcgttttggc ttacgataat atcgaaacgc aggaagtaat
    27301 gacccatcgg gatgccgata tttttgaaaa tatttttatt tggcagggcg actatcgcct
    27361 cctcatcggc attataaaat acatcgaaga taaaatgaat tttgcgcacg attcgcgcgc
    27421 gatcggcgta caaggcatca ttttagtcga ggataatgtg aagttttatt cctcgtatct
    27481 accgatcatt tataccgaaa tccatcgtca gtcgcagcga ctgatctccg aaggcatcaa
    27541 tctcacgcat aaatttttgc gtatgcgcgc acgcccgaaa attttgttat gccataccta
    27601 cgaagaagca tggaaatatt atgaagagca taatgaatat atactcggaa tgatctccga
    27661 cgtggacttt ccgcgtaatg gcgccgtcga atccaaagtc ggtttgcttc tcgcacggac
    27721 gatacgcgaa aagcagtcgg atatccccat acttttgcaa tctaccgaat cttcatatag
    27781 cgaagaagcc cgtgagatcg gcgccgcttt tgttctcaaa gattcgcccg ttttgcttca
    27841 ggaactcagc gacttcatga aacaaaattt cagcttcggc gattttgtgt ttcgtctgga
    27901 agacggctct gaagtcggcc gcgcatcgga tctgagttcg ctcgaggaaa cgctgcatag
    27961 cgttcccgct gaaagcgttc aatatcacgg cgaacgcaat cacttttcca actggctcaa
    28021 agcccgcact gaattttggc tcgcccataa gttaaaaccg aaaaaagtta cggactatgc
    28081 taccgtcgaa gatattcgcc agcatgtcat caaagtactc aaagattacc gcgaaattcg
    28141 tcaacgcgcc aacattaccg atttcagcaa agatacattc gacccggaaa gcagttttgc
    28201 ccgcatcggc ggcggttcgc tcggcggtaa agcgcgtggt ctcggtttcg tcaaaacatt
    28261 gctcaataat tacaacatgc gttatgcttt tgaaggcgct aaaatatttg tcccggctgc
    28321 cgtggcgatc ggcaccaatg tcttcgatca gtttttggag cataataatt tgcgtgagtt
    28381 ggcgttaaat tcgactgacg atcgtgaaat cgctgactcc tttcattcgg cttcttattt
    28441 tccgcccgaa gtgataacgc aattacgtga atttttggaa atggtctgtg ttccactcgc
    28501 cgtacgctcg tccagtttgc ttgaggattc gcaatatcat ccttttgcgg gcgtgtacga
    28561 aacctacatg atccccaaca atcacgccga tacagagacg cgactccagg agctgctcac
    28621 gacgatcaaa aaagtgtatg cttcgacttt ttacaaagcg accaaagatt atatcaaaca
    28681 aactgcctac cgccttgaag aagaaaaaat ggcggtgatt attatgcgaa ccgtcggcgc
    28741 ggcacacgat aatcggtttt acccggattt ctccggcgtg gcgaagtctt ataattttta
    28801 cccgatgccg ccacagaccg ctaaagatgg aatcgcatcc atagcgctgg ggctcggtaa
    28861 aactatcgtg gacggaggca acaccgttcg tttttgcccc aaatatccaa aacatttgat
    28921 ccaacaattt tccaccaaag aagcgctcaa aaccagccag accgaatttt acgctatgaa
    28981 gctcggcgct acggaagtgg acttgtatcg tgacaccaaa gatcgcctga ttgggaagtt
    29041 tacgttggat acggctgaaa aagacggatc attacgtttt tcaggttcga cctattctat
    29101 tgaaaatgac gcggtgtacg acggcatttc ccgtgccggt atgcgtattg ttacgttcgc
    29161 gccgatttta aaaaataaaa tgtttccact gcctgaaatc ctggacttgc ttttggagct
    29221 tggaaagtgg ggtatgggtt cggaaatcga aatggagttt gccgtcaata tgtccactcc
    29281 gcccggtaag ccgcgcgaat ttggcgtgct gcaaatgcgt cctctggttt tgcggttgga
    29341 aaatgacgcc ctggatgtga caccgtaccg gcgggaagaa atgatttgtg aaagtacgca
    29401 agtgctgggt aacggcgtta ttgattcgat ccgcgatatt ttggtcgtgg attatgatcg
    29461 ttttgatcgc gcccgcagca aagaggtcgc gttggaaatc gctcagctca atgaaaaatt
    29521 agttcgcgat ggcaagcctt atgcgttgat cggtgtggga cgctggggca gtttggatca
    29581 ctggctgggc atacccgtac gatgggatca gatcaatggc gcccgggcga tcatcgaatc
    29641 cagttttcgt gattttgatg tggatccgtc gcaaggttcg catttttttc aaaacatcac
    29701 cagttttcaa gtcggctact ttaccgtccc gcatcatgcg gcctccagct ttgtagattg
    29761 ggcttggcta gccgcgcaaa aagccgaaga agagctgaat ttcacaagac atttgtcgtt
    29821 tgaaaatcct atcgtagcgg tgatcaacgg acatgatgga aaaggggttt tactgaaacc
    29881 gaaacctcaa ccgaaagaaa gtgcggtgtc tttatgaaca cgcaatcgct tgagtttgtt
    29941 caaaaaacaa aacaggaatt gaactgtaaa atcattcgca cgatgttgaa ttatgcactc
    30001 gaaaaagcgg ggcctgacgc cgaacgcaag ctgatcgagc gcatggggct gacgcgcgaa
    30061 tatttgcaaa atgaaaacaa ttgggtttcg tttgacgttt atacgcatgc ctgtgaggct
    30121 atgaaaacga ttttcaacga tgaccgcatt atgtaccgcg tcgggattta ttcgttttcc
    30181 aatcttgaaa acttcggcgc tatcgccaaa atggctgtct atcttctcaa accggaaagc
    30241 atttaccggc gaacggctga agtcgccaac caggctgtca aattcggcac ataccgagtg
    30301 gtcgaaattg aaagtaatcg ccttctgtta gaattcaaaa atcgcgaggg ttataaattt
    30361 catcaacata attgcgatta tcgtctgggc gtttttgcag ggataccgca acttttcgga
    30421 ttgcctatcg cacgtcatcg cagcattcaa tgtgtttccg atggtgcgga tcggtgtttg
    30481 tacgagtttc gttggttatc gcgtacatgg gaaatattcg gacggcatgc acgaatcggt
    30541 cttgttatgg gtattttatt tgcgctcgtt ttgaccgttc tcaaccgatt cactttttca
    30601 cattttagcc aaccggcttt attgatcagc ggtgcggccg ttttgtatct gatcggtatc
    30661 atactcgaca tgaaacatac ggcacagtac agtgcgttta ttaattcggg caaatccgat
    30721 gagatcgaag aagcgatggc gtttgctaat caaaaatatc aggaagcggc cgacaatata
    30781 accaagcttc aagcgatcat cgaaggtaat gccgtacttc aatcgaaact tattaagtcc
    30841 gagttggtcg aaatcgttct tgagatcata aaacataatc tcggttatga tcgcgccatg
    30901 attctcttta aaaacaatac gcaacctttg ttggattcgc ctaaactcat cggcaacctc
    30961 agtccggaga tggagcaggc gctgcttaat tataaaatca accttacgga tgatacggcc
    31021 atcaatacac gggtttttta tagcggcgag cccgcattga tcacggatat gtctgaagtg
    31081 aaaaacagta ccgaaatgaa ggctgtgcaa ttgtccggta caaaatcatt tgtcatttta
    31141 ccatttaaaa tcggttcgcg tgtggtcgga actttgacgg ttgataaaat cagttctccg
    31201 aaagccctgt cacgcgaaga tgtgcgtatt ctcaatacac tcctcaatat gttatccgtc
    31261 gctttggaaa acgccgaatt gtatcaggat ttggaagccc gcgttcgcga acgtacagcc
    31321 caactcaacg aaaccaacaa aaaattggaa tccgcttacg atgagctcaa acaagcacag
    31381 gcacaattat tacacaatga aaaaatggcg agccttggta aattggtagc cggtatcgcg
    31441 cacgaaatga ataaccctgc cggcatactt tccggatata tcgaacttat ggagcttcat
    31501 gccaatcggc ttcgcaaaga tttggaaatt ttggatcaat ttagcgcggc gcacgaagat
    31561 attgccggta ccatacaaca catcgagaag aacatacttg aaattaccga ttcgatcgag
    31621 ccgtgcaaac gatcggtctg gcgtatcaag agtatcattc aggatttgcg caatttttcc
    31681 cgtttggatg aaaacgaact tatgcccgcc aacatcaatg aaggccttga gaatacgctt
    31741 aagtttttta aaaaccaggc cgaagggcgc attcgcatca taacgcattt caatccgatc
    31801 ccacaggtca cttgtttccc cggtcatctc aatcaagtgt tcagcaatgt catttcaaac
    31861 gctatcgaag cgattgatgg aaccggcacg atcgaaatac acacttctaa aagcgaacct
    31921 acatcggatg tgccgactga tcatgtcgtc gtcaaaatca aagattcagg tcatggcatt
    31981 ccggctgaaa ttcagggtaa aatcttcgat ccatttttta ccaccaaaga tatcggcgca
    32041 ggcaaaggat taggtttatc cattgcttat ggtatcatca aacaacacag cggaaatatt
    32101 tcagccgaaa gtgaacaaga tcaaggcgcg tgtattacga ttcgcattcc gatagaatat
    32161 ggctatgaaa gaaaaggata aaccgtatga gcagtgatcg cgtaatcgaa ataaatacgg
    32221 atagcgtggc atgggaatca tccgacagta aaggcgtttt ttataaatct cttcgctatg
    32281 ataaggaaac caaagcgggc gccgttttga ttcgtatgga accgggatcc gattatcctg
    32341 aacatgagca tacacgcggt gaagacttta tcgtattaga aggtgaattg atcatcggga
    32401 ataaaacatt ccctgcacag agttacgtat acagtccgcc acaatccgta caccacccgc
    32461 gtacggaaaa aggatgtgta ctttttgccg tatttcacgg gaaaatcatc aaccttaaat
    32521 aacctcccat gatcgctgta catctggctt tgttttttgt gtcgcttgtt ttcggcgtca
    32581 atgcggtgac ttccaaatac gccatgcgtg aaattcaccc tatggctctg gtgtttattc
    32641 gcatcgtaag cgccgccgcg attctctaca tcatacaacg gctttggatc aaagagaaaa
    32701 ttcaaacgcg cagggattat tttttactgg cggggtacag catcgtcggt atcgcgatca
    32761 atcaggtact ttttcttaaa ggccttgcct tgacaacagc catcaatgcg accgtactga
    32821 tcacgaccat gcctgccgca acgatcggta tcgctatact tctgcgcaaa gaaccgccaa
    32881 gctttcgaaa aattatcggt tcgatgattt cctttagcgg cgttatcctg ttactcggca
    32941 gcgaacgtat agattttagc aatgattatt ttgtcggtaa tctgctcgtt tttcttaacg
    33001 gcatttcgta cagtttttat ttggttatct cacgggatct tttgaagcgt tatcaccctt
    33061 cgaccgtaac aacatggata tttgtttttg gcgctgtcgg tgttttcccg tttggcatcg
    33121 gcgaaatatc gcgcctggat tgggcgtccg tcagtctcac ggcatgggca tgcgtggctt
    33181 ttttgattat tttttcgtct gtgatcgttt attttataaa taactgggca ctgcgcaaaa
    33241 catcgtcatc ggttgtcgct gtttatattt atgtccaacc gattgttgcg gcgttgattt
    33301 caacacaaat gatgggtgaa accctgcgca tggaaacagt aatcgctgcg ttgctgattt
    33361 ttggcggcgt atttatcgtc acgcttaaaa tgcgagagga aatgaaaaaa gaaaaaagta
    33421 acgccaacaa agaaatagat ctcgtcaaag aatccatgat catgaaagaa gattgatcgt
    33481 ttaaagactt gttcacggta ttgtgctcct aactcatcgt tgcttacaaa tacatcacat
    33541 ccctttcctt ttttgaaaac ccgatatccg gtaaggcctc aatcttaacc aatcaaacct
    33601 ctttcactga gatataaaaa caacttgact cttcatttgt attttgcttt atttacaaag
    33661 tactttgtaa tgcaaagtta ataaataagg ttttacaatg aataaatcca acgcaaaaga
    33721 acaacttgat tacatccaga ccgtattgga tcgggccact gtttatacca atatctcagg
    33781 ttggtcagcc atttcggtag gccttttggg cacgatcgca ggcttttact cagaatgggc
    33841 gctcaaagga tcgctggact accaaactta tatcgcatcg ttggttgggt gttggagttt
    33901 ttgttttatc gcttcgatcg cattagcggt atttttttca gcccgacgcg caaagcgtat
    33961 gaatgaaaaa atctggggcg aaccgatcaa acaagccgta cggcattttt cagtattcgt
    34021 ttttatcggc ggcgtgcaaa ctgcggcttg tacattctat catgcatatt atttgatccc
    34081 gtcggtttgg atgctctgct acggaggcgg tatcattgtc ggcggattgt attccgtcaa
    34141 agaaattcga atcctcggat tatttttcct agccgccgga agtattgctc ttttttcacc
    34201 ggtttggaat caaatcgctt tgatgagcac cttcggtttc ggtcatatta ttctgggact
    34261 gatcattctg aaaaagtata accaaccgtt atcgcacaag tcttagcgac gaaacacttt
    34321 atgaaaactg aaattcatca tattgacgaa gtcattcacc aaaaagtgcg gcttggtatt
    34381 atgaccattt tggctgcacg gcgcgatgcg gattttacgg agctcaaaca agaattaacg
    34441 ctttcggacg gcaatctcag tacccacctg tcattgcttg aaaaacatca ctacatcacc
    34501 attcagaaac agtttagcgg aaggaaacct cagacaacca ttgctataac ggaaatcggt
    34561 aaaacggctt taaaaaatta ccttaccatt ttacgaaaga tcatcgaatc atgaatcatc
    34621 gcatttcccg gatcagcatc tttcacaagg tgccaagccg ccttcagtgg ttgatcgcat
    34681 atattggatt tctatcgctc gttagcattt cggcgtatag ttatggtttg cctcgttggc
    34741 ttgatatcat tcccttttat gataccatcg gccacttcgt gcttatgggc attttgggaa
    34801 ttctcacgtt gctggtttta cgacgcacca tacgcataaa attagatatt agcgtagctc
    34861 tcgctccgat agtgatcagc cttatttcta ttgcagacga atatctacaa aaactatccc
    34921 cgcatcggac gtttagttac cttgacatgg cggccaatct gatcggtatc tggacttttt
    34981 atttcatcta tcgcttttat gccaaaagta aacgtgtaaa ggaggaatcg ttatgactac
    35041 gccgcgtttg tttgcaataa tgggtatttt tttctgcacg atgatcgcct ggtttatttt
    35101 agcgggcagt atttcgtttc gtaccgatga aaaaagattg agcttcggtc cgcgtatggc
    35161 ctcgcactgg ggcggcgtac acaaacaaga aacaccccaa ttgttttatc atgtcaaaga
    35221 atgggcggtc accaaagaga ccagtaccaa tgccgccggt gccagcgtga cggtagaaaa
    35281 gagttcggag gtggatgtca aaaaaaatat cgaatgcggt aaaacacaaa ttaacgctga
    35341 ttttgaagtt gattaccggc aaaaaggcct cttgtggtac agcgtgtata aagtacgttt
    35401 ttccggttta tacacattta ccaatccatc cgctaaagaa gaagatgtaa cgattgactt
    35461 tacttttccc gccgctcagg cgatctatga cgatttcgtt ttccaatggg atggccaaga
    35521 gctgttaaaa acagaaacca ttcgcgaatc ttccgatgat ccttatcgtc aagacagcaa
    35581 accgggttct ttacgtgcga atatcaagat gaaaccgggt cagatcgctg ttttaaaagt
    35641 aggttatact tcccaaggaa ccgggacatg ggaatacagt gtcggcgaag gcaccaaacg
    35701 ggttacggat tttcaattga ctgcacagac gcatttcaaa gaatttgata ttccggaatt
    35761 cagtatttct cccggcacta agactgaaac ggcagacggt tggtcattag tttggcagta
    35821 caaagacatc atctccgacg ttcgtatcgg tctcggtatg cctaaaaaat tagaccccgg
    35881 accggtagcc gaacgcatca gcttttttgc tccggtatcg ttactatttt tcttctgcat
    35941 tcttattatt atcggagcga tacgtgatgt gcgaatgcat ccgatgaatt attttttcct
    36001 cgcggccgcc tttttttcgt ttcatctgct tttcgcgtat ctcgtggatc atctggcact
    36061 tgaactttcg tttgcgattt catccattgt ctccgtcaca ctcgtcgccg cttatatgcg
    36121 ccttgtaacc tcgtggcctt ataccgtacg tgtggtgatg cctacgcaag tgatttatct
    36181 gatcctattt tcctatagtt tctttttcga aggctacacc ggactcacga tcacgatcgg
    36241 tgctatcgta acgcttttta ttcttatgca gatgacaggt aaaacggatt ggaatgctat
    36301 tttcaaaaaa gatatcaaat cataaaaatc gtatttagca aaaacaaaaa acgccgctcc
    36361 tattcgggcg gcgtttttta ttgctcgaaa atctatgatt agcgaagtgt caaaccaacg
    36421 ccaatgcttg tggaacgata tttgccaaaa ctcatttccg gattcacatt gatcaaccaa
    36481 agcagacgga tatttgcacc gacaataccg cgcatactgt tttttccatc cacagaaaat
    36541 ttcaccttag ttccttcagg cacgccgctt gctgttccgg tggtttcgta gttaatatcc
    36601 atagatgagg attcgatacc gacgccacca tacaagccga gtttaatgat taaaatgttt
    36661 aaactcttac cggcaagtat atcaaaggat gtcgctttag cttcgaacac atcaccaata
    36721 gacatttttt gataaaaggc acccgcagca atatccaaag gcaagctgat cggcagccat
    36781 tgattgatgt tatgtttagc gcccaaacca aacaatttta ctttgccaag tttaccggca
    36841 ggaatacccg gtacaaaacg aagaatcaca tccgttttga aaggcacacc tacagtcagc
    36901 tgcggagcca caaaaaaacc gcttgcgccg gccggagcat ctataccacc cggaagaaca
    36961 atattcgttt gatcatttgg aataccgcca agataatatg tggtaggatc tttagatccg
    37021 atagctgtat ttgcttcgtt ggatgcggcc ggtacatacc cgttgggaac aaccggggtg
    37081 tatgtttttt cagaggaagg ggcaaaagcc atagccgtca ctaattggaa atcaaagcca
    37141 agaacactgt gagtttccgg tgaacgaaaa ataccgctat tcatcgctac accaaaacca
    37201 gaaacaatag gtttgatata agattgagcg ttggcgctag ccatgctttt gatctttgct
    37261 tccagatcat cgcccgccat ggcacgccca gtcgtaacgg ttaaaatcaa acctgcaagc
    37321 atcagtcgca aaaatgactt catgtctctc tccttcaagt aatttagtga aaaatgaaaa
    37381 tctttaggtt aatgagaagc gtgtacaaat ttaactgtaa ctttgcgtaa ttgcaatacg
    37441 cataattact gtgaaaataa ttaaccaact agcatgccaa actacagaaa tcgaaaaaac
    37501 aacaggttaa ttatgataaa aaaacaaagc gtcgcatata tgcgattatt cacagaaaac
    37561 atgcgtcgca aaaacgctac ttttttcgag ctacgatgat agccgaactg ccaaaactca
    37621 ggagatgggc cgttttgatt tcatattcaa aacgtatgag tgactcttgc catttttcaa
    37681 accaattgga actttgtatg agcgaggtta acccgccatc aaaaacttca tgccattcag
    37741 gaaaaaatcg catgcctaca aaattcatcg cgcggttata tttccgccaa atggcgcgcg
    37801 ctaccgagtt gggtggataa taaaaatcat gcacggctat cacacctgat gacttgagat
    37861 gcggatttat attggaaagc aaaatctcgg gatcaacata tttagggatg tacgacgaaa
    37921 taacggcatc aaaaattcca tcaagtttca catcttccgc attaccgagt aaaaacgaag
    37981 cccgaaggtt tttcgatcga atacgacgtt catatatttt caaataatca tctgtaatat
    38041 ccacgccgac aatgtcggca tccggacatc gtttggcaat tccttccgtc acgatacctg
    38101 ttccacaggc cagatctaaa acgcggcgac tgtccttggg tatgtgccgt aacaacatcc
    38161 gcttccagtg ataatctaaa cctaaagtaa atccgtgtac gacgcgatca taggaatggc
    38221 cggttcccgg aaaaaaagaa cggatgatgg atgagcggtt ttttatagta tcccttccga
    38281 catccttcat ccgtgtgttc cttagaccaa aattatattt atcttatttc ttcttaaaat
    38341 ccaacgaaag tgaattgata caataacgca atcctgtcgg atccggtccg tcttcaaaca
    38401 catgaccgag atgcccaccg cagcgtgcac agtgcacttc tacacgattc atcccgtagg
    38461 aggtatcgtg cgtttccgat acgacttctt tgtccgccgg ttgataaaaa ctgggccacc
    38521 cgcttccgga atcgtatttg gtttgcgagt caaaaagctc cagtccgcaa gccgcacata
    38581 tatacatacc cttttcatgg tgatcccagt attttcccgt aaatgcgcgc tcggttccct
    38641 gctgacgcag gactttatac tgttccggcg ataattcttt tttccactcc tcttcgcttt
    38701 tcacgatctg atcctttcgg gttgagtttt gtaccgtatg ggttgacgga tgctgtgcat
    38761 tggaacacgc cgtcatcatg agccaaactg caaaaagtaa cttcttcatt cttttttctt
    38821 tcgatggttt ctcgacttaa tctaacaccg agacgattgc aatattccca tcatattgat
    38881 attgtgatcc gtatcacacg cattggtatc catcagggct cgtttttata tataacccca
    38941 tctttcatta caaatactac attacgcatc acagctatat tttccgtcgg atcaccattt
    39001 acggcaataa gatcggccat ttttccatct tctattgatc ctgtgatttt ttctacaccc
    39061 aagagcattg ccgacgcata cgtcgctgca cgtaatacat ccgtcggatt cattccgtac
    39121 gcggtcatcc gctcaagttc ctgtgcattg gtacccggta attcaaacgt atccgtcccg
    39181 aaaacaattt tgaccccagc cttcaaagca cgctgaaacg aaagcgtatg ttgttttacg
    39241 gtgcgcgcca gtttgcgttt gttttcgtcc gacaccgggc taaagataat accgtcgcgc
    39301 cacaattcgt ataccatcaa agtcggtaca taataaatat tctttttcgc catccgccgg
    39361 catgtctcgt cactaagata gaggccgtgc tcgatcgaat tgacaccgac agcaatcgcc
    39421 agtcgcgctg ccgtatccga atacacgtgg gccgcaacac gtaaaccgtt atggtgcgct
    39481 tcatccacaa gcactcgtaa ttcgtccggc gtgtaatgca ttgctcccgt cagcgaatcc
    39541 ttacttgttt ctcttttttc atacgattcc atatatattt tgatcacatc cgcaccaagt
    39601 ttggacagac gtcgaatttg cgtacgaatt tcatccgcgc cgtcggcgtg aaatgcaatc
    39661 ggcggaaact cataatacgg cgaataaccg acaagatcgt acgcccccgt cggaacaata
    39721 ggttgaatgg ccgttaatat ccggggaccg ggaacgatct ttttttggat agcatcgcgc
    39781 aacgccacat ctgccaaacc tgcgccttcg ttgcccagat cgcgaattgt cgttacgccc
    39841 gcctccaaag ttttccgtgc caatacggtt ccgtaaactg cgcggtattc cggagtttca
    39901 cgcaatatct gcccatcata atccccgggg tgtaagacaa tatgcgtgtg tgcgtctatg
    39961 agaccgggaa gtaccgtttt ttcgcgtaaa tctatgatct taccgttata cgatgggcga
    40021 ttatcgtcat ctacccggac gattcgatca ttaaaaacga cgagaagctt atttttttga
    40081 atacgcgaac cgatcccgtc atacaaacgc cccgctctga taaacagcgt atcctgttgc
    40141 gcatgcagca actgcatact caaaatgatc aacccgatta aaattcgtcc catgacaaat
    40201 tccctttccg ttatctaaaa tagaatggtt cggaaaaaac ctttttactc gtattttttt
    40261 gcctagtatg aagcgatatg gaaaatattc gatttatatt tataacatcc aattcaaatt
    40321 tgtcttatta ctttcacacc acttccccgt tgcttatccc gcgcaatatt cataaaatag
    40381 tttccgaata attaactttg gtatatttat gcccatcata gcgccttcta ttttatcggc
    40441 cgattttgcc aacctttcgc gggatattcg catcgccgaa gaatccggtg cggattggat
    40501 tcacgtcgat gtgatggacg gacgttttgt acctaatatt acgatcggtc cgatggtcgt
    40561 cgaacatctc cgtaaaatca ccaaacttcc gctggatgtt catcttatga tcgtcgaacc
    40621 cgaaaaatat atcaccgcat ttcgtgatgc cggcgccgat atcatatctg tacatcagga
    40681 agcgtgccct catctgcatc gcacgctcca gcaaatacgc caatccggcg ctaaagccgg
    40741 cgtggtgctc aatccgtcca cttcgcacga cacgctgcag tacgtacttg atgatgtgga
    40801 cttgatcctt gttatgtccg ttaatccggg atttggcggt caaaaattta ttccccaagc
    40861 ggcacataaa atacgcgcat tgcgaaatat gctgggctca cgtaaggtgt ttatagaagt
    40921 ggatggcgga ataaataatg aaacaggatt gcaagttatt caagccggtg cggatgtgct
    40981 ggtagccggt tcgtatgtat tcggtgcaga aaatccaaag tccagaatac aatcgctaaa
    41041 acaattacac gcttcaacaa ccggcgttta atttttgagc cgatcaaaac aagtacttta
    41101 ttgtttttaa aaatatgttc tacgcttaag ggtataattt atgatgcctt tgcctccgtt
    41161 tgaatttgcg gcgcccgcga cattagatga ggccgccaaa ttattggatc agcataaatc
    41221 gcatgccaaa attttggccg gcggtacgga tctcattccg agcatgaaaa acaaattgtt
    41281 tgaaccgcaa gtgctgatcg gcatcggtaa tattcccgag atgaaaaaaa tcgagtacga
    41341 tcctcaaaaa ggactcactc tcggcagttt ggtaaccatc acggatatcg gccatcaccc
    41401 gcatatcacc actcattatc ccgttctttc cgatgcggca cgtaaaattg caactccgct
    41461 tttacaaaac aaaggcacga tcggcggcaa tattcttctc gacacgcgtt gttattatta
    41521 taatcaatcg catttctggc gtaaggccat caataattgt ctgaaaaaag aaggcgatgt
    41581 atgccgtgtc gctccgagcg gtcatcgctg ttatgcgatt tcttcaagcg atactgtacc
    41641 ggtattgtat gcgatgaatg cgtcggtaac actggtccat gcgcacggtc aacgcaccgt
    41701 accgctgcgc gcgttttatg aaaacgacgg aatgaagttt aacaaattgg aacccggcga
    41761 aatattatct caagtgcata taccggtgtt accaaaaggt tttcgcggtg cttacatgaa
    41821 gctgcgccgc cgacagacca ttgactaccc catgctcggt gttatgatcg gtttgcaaat
    41881 ggatgataaa aatatttgcc gcaatgcgca gattgtttta ggcgccgtat tttctatgcc
    41941 ctacgaagtc aaagccgcgc aggatatgct catcggtcaa accattactg aagacctgat
    42001 cgaaaaagtc gctgacatcg cacaccagga ggccaaacct ctggatttga ccaatttccg
    42061 accggcctat cgaaaacgta tggttcgcgt ttttgttaaa cgtattttga atgttttgac
    42121 acaaaaaaac acctacgtgg aagaaatttt agcttagttt tttgaatcac atataagatc
    42181 tatgaatatt ctcgttatag ggtccggcgg acgtgaacat gctttggttt ggaaaatcgc
    42241 ccaatcgccg cgtgttaaaa aaatctactg tgccaacggc aacgccggca tacgatcgtt
    42301 agccgaatgt attgccatta agccggaaga cattcatgca ctcgctgatt ttgccgcatc
    42361 tcatacaatt gatttgactg tcgtcggttc agaaactccg ctcactttgg gtatcgttga
    42421 tctttttgaa tcacgcggac ttaagatttt cggaccctcc gccaaagcgg ccgaaatcga
    42481 aggcagtaaa gtcttttcca aacaattgat gaaaaaatac ggtattccta ccgcagcctt
    42541 tgaaacattt gataacgccg atcaagcgag atcatggata cgatcttacg acaaaccctg
    42601 cgtcgtaaaa acagatggat tagcggccgg gaaaggcgct atcgtctgca tgagcacgtc
    42661 cgaagcgctt gacgcggtgg atcaaatcat gtcggacaaa caatttggtt ccgccggatc
    42721 acaaatcgtc gttgaagcat ttatggaggg tgaagaagct tctatttttg ccgtaacgga
    42781 tggaacgaat tacgtcgtac ttccggccgc acaagaccat aaacgcgcac tggacggaga
    42841 tctcggaaaa aataccggcg gtatgggcgc ctacgcaccg gcacccgtcg ccacccatgt
    42901 ggtgatcgaa cgtaccaaaa aagaaattat cgaacccaca ctggccgcta tggcccatga
    42961 aggtcgaacg tataaaggtg tgttatattg cggcatcatg gtgactcccg atggtcccaa
    43021 agtagtcgag tttaattgcc gttttggcga tcccgaatgc caagttttga tgatgcttat
    43081 atcgtccgat atcgtcgatc ttttccagtc tgtcgcggat aacaatcttg atccgtccgc
    43141 cgtaaccata agcgatgccc atgcagcttg tgtcgtaatg gcctccggcg gctacccgga
    43201 cgcgtacgcc aaaggaaaaa tcgtcaacgg attacatacg ccattacctg aaaattgtgc
    43261 ggtatttcat gcgggaacgg attggaatga aaacaacatt atcaccaatg gcggcagagt
    43321 cttaggtatt tccgctacag gcgccgatct aaaaaaagct ttggataccg cctataccgt
    43381 aacgcgagaa attcattttg aaaatgcatt ctatcgcaaa gatatcggac atcgtgctct
    43441 aaaaaaatga aaccactata ccatatattt ttttgcacca ttattgcagc aagtatcggc
    43501 ggagtcatcg cgttggctca agcaccgatg aaactcagtt acggtacggc taacaaagaa
    43561 gtcatacctg ttttcacggg tgatcaggaa tacgtttcta ttaaagacgt gatcgcactg
    43621 ctggatgccg gtttttttga aaataccgcc aaaaagaaaa tggtgtttac ttctaatcag
    43681 caccaattta aagttacagg tttgaatgca tttgtcgtcg tagatgaaaa aacaacctta
    43741 caaatgccgg ttaccacgca gtatatagac ggcgcgatct ttgttccggc tgtttatttt
    43801 tttagtgcta tcaatgcata tctctctacg ccggtcgtca tcaatggcga aaaaataatc
    43861 gcaaaatccg caccggcaat caaagatacg attaaaacct cggctgtcag tatttcccca
    43921 aacctgaaag acgttatttt cgaaaagaag aaaaacggtc ttttgatgcg catcaaaacg
    43981 gacgggcgct ttcaatcgtc agacgttgaa atttggcgta ataaaaactg gttatacgtg
    44041 acggtctccg gcgggtatca ttcggacttg ttggccaaag acatcaaact atccgaacag
    44101 tacaaactga tcaaagaagc attgatattt cagcataaaa actcgacaca gttttcattt
    44161 cagcttaatt ccgatattgc cggttacgat atctccgtgg atgataagaa ggatgagatt
    44221 tacgtcgcat ggcgcattca tcaaagcagc gatctggagt ttgacaatgg cggatcaggg
    44281 accggtgcga ttgataaaga ccaatggaag atcaataaaa ttgtgattga tgccggccat
    44341 ggtggcaaag atcatggcgc ttcgggcaaa ggtgggacga aagaaaaaga tattacgttg
    44401 aacattgccc tcaaagtggg taagatcatt gcagagcggc taaaaatccc tgtcgaatac
    44461 acacgtgata cggatacgta catcacgctt aaaggacgta cgcaattcgc caatcaaaag
    44521 aacgctaaat tattcgtcag tatccattgt aactccaata aaagccgtaa ggcccatgga
    44581 tttgaaatct tttttctatc gccaagccgc aatgccgaag cgttggctgt cgcgcgaaaa
    44641 gaaaacgaag tgattgatat ggaagacgat aaacatcatt acggcgattt tacggacgaa
    44701 aaatttattc tcgccaatat catgcaaagt gttttcgtca aagaaagcga agaattagct
    44761 tcttatgttt ccaaaggtat tgataaacaa gtggacatca ccaatcgcgg cgtaacgcaa
    44821 gcgccgtttt atgtattgat gggcgcctcc atgccttcga tactcgtcga aacagcattc
    44881 atatccaatc cggacgaaga aaaatttctc aaatcggaag caggtcagaa aaaactttcc
    44941 gaaggtatcg tggacggcat caaaacgttc attgatatat acgagcgaag caactgatct
    45001 ttctcaccta accgccaagc ccagccttca taaaatcaat cttaacaata taccgtattt
    45061 atggtattgt gaagcctacg tagatatgcg tatgttagcg ctgaatgatg gtaattggtt
    45121 cttatcatcg aaaaactaca actctcgaat aaaatagttt gtgcaaactc acatgacgca
    45181 gtcaacacct gtattatcgg aagcgctacg taaacattta tccattgcgg ctaatgaaaa
    45241 aaaccgtgaa ctaggaatga aatattattc tgaaaatatg gatgactacg attttctagg
    45301 atataacaat gaaaatgatc acgtggcttt cgtttttcgt tgccgtcgtt cattttcgtc
    45361 gctaacactt aaagttcccc gcaaacaaag aacagctgaa aactaaaaca ccgtttttac
    45421 ggttagcata aatccctctc aaatcccgat gtataacacg tcgggatttt tttatcagcg
    45481 gctatacact tttaatactt caaaaagacg atccatgtcg cttttttgat tataaagata
    45541 gggagcgaca cgaatagcgt ttccccgaat acttacgtat atcttgtttt ctgataattt
    45601 ctgcccgatg tcagacggca tcgaagttgc atgacgaatg cccaacaaat gcggaatgcg
    45661 ttcactatcg gaaatcgtta cccaacctaa actatgtgca tattgcgcag catacgatgt
    45721 cagatcaagc aggctttcgg atattgctga aacttcccat gttatgattt gacgtaatcc
    45781 ggcgatggcc atgggcacat tgataaaatt agaaaactca cccatgtcat agcgccgcgc
    45841 accttgacga aatttgtccg tgtattcgat taaccgggaa aaattttcac tgccaaaacg
    45901 attgagccaa gaatattcga tcggagtgcc ttcctgccat ttcggatcaa catataaata
    45961 tcccaacgaa taaggtccga gcaaccattt gtatccgacc gaaatcaaaa aatcagggcg
    46021 gattttttcc ataggaatcg gaaatgcgcc cagcgattgg ctcgcatcaa taaccagtgc
    46081 agcctcggct gaacgagctt tttgcgagac acgctccaaa tcaatccgtg caccgtccgt
    46141 ccagtgacaa ttaggaacgg ctactacggc tgttttttca tcaatatttt cacagatcgc
    46201 ttctgtccaa ttcaactgat tggttctatg tacgacccgt agcgatgcac cgcaatcttt
    46261 tgctttataa taccatgcgt aataatttga gggaaactct tcgtgcaaaa ccactatatt
    46321 ttggtccgaa cgcaccgtta tgttttttgc cgctatcgcc atgccataac ttactgaagg
    46381 aatgatcgcc atatcatccg atgctacgcc gataatctga gcgataagcc cgcgtaacgt
    46441 ctcgccatct tcaaaccaat gttgcggcgt ataattccat ggcgacgatt ttgcacgtac
    46501 cgattgtaaa ccggcttcgg tcatggcacg aagttgcgga gcaaaaaacg catgattgag
    46561 aaaggtcacc tcatcgggga tatcaaaaag atgcttttgc agcggtagtt ttttggtcat
    46621 aatatttcca aatgattttt ataaaaatgt cccaaaagtt ctaatacgta cccgttaaat
    46681 aaaaacggaa taaaatattt cacttttggg acaatcgtta ttcgtacata tcaaactact
    46741 taccgaacac ttctttaaaa agcgctgtca tatccgccca tgaatcggcg tcggccttag
    46801 cattataggc taaaggtaag ttgaattttt tacccagtga atcaaccgcc ggattagtaa
    46861 agccgtgtac ggcgccgtca taggatttga atacgtagtt gactttagcg gcatccattt
    46921 cttttttaaa gccttcgata tctttttcag aaacaaattt atcggcagcg ccattacata
    46981 ccaggacacg acttttcact accccctctt tcgccggctc tgctgcaccc aggcttccgt
    47041 gaaaactcac gaccgctttc atatcaatac cctgacgggc catattgagc actacgccgc
    47101 cgccaaaaca atatccgata gccgcgatat gggatgcgtc cacattagga ttattcttca
    47161 atgtttcgac cgctttttca aaacgcgctt tagcgccttt gaagttcttc atagtttcgg
    47221 atgcaaattt tccggcgtct tgcggatgct ctgctttttt tccatcgccg tacatatcta
    47281 ccgctaaagc cacatatccc atttcggcaa gcattttagc gcgttgacgt gcgtattcat
    47341 tgtgacccca ccactcatgc acgacgatca caccgggacg tttgcccgtt tgcgctgcat
    47401 cataggcgat aaaacctttc atggtaatat tatccgccgc ataactgatc tcttcacccg
    47461 taatgttctt agccgcgacg gtcgttgctt ctgcttttgc cggttctttt ttggccggtt
    47521 gacagctcat caaaacagcc aaagccatga cggcgatacg atggattttc atacgcacac
    47581 tccttgtgtt agggttaata ttttaacaga ggaactgtaa aacctataag gattttttcc
    47641 aagcgtgcaa ttcaaagaga agatgttgcg aggccaattg atattcgccc tgataaattt
    47701 cagtggcttg ctttttgaac ccgcttagag ccagattcaa aacgataccg gctattttat
    47761 gaaaatgcgc atgcaattcg cgaaccgtag tgtaaaaatg atcgcctttg taatccgctt
    47821 cgatcatgtg aatccatttc ccaaacgcgc attgatcatc aacggacact ttatccacag
    47881 cccacacact atgcccggtt tcaatcgccg ccatgagacg ttctttccac agctcgtgag
    47941 ccgcgatggc attttctatt tcttccaaca gcgcgtacat caatcaattc ttatccgctt
    48001 ttacgcgatc cataaattga ttaaaaagat aatcgctgtc atgcggtccg ggactggctt
    48061 ccggatggta ttggacggaa aaaagcggca gctcccgatg tcgaaatcct tcgagtgtgc
    48121 cgtcatataa attgaaatgg gtaagttcga tttctttctc attcaatgtt ttaggatcaa
    48181 cggtaaaacc gtgattctgc gaagtaatct cgatgatccc tgtggctaaa ttctttacgg
    48241 gatgatttgc tccgcgatgg ccaaatttta atttatatgt ttttgcacct aaagcgattc
    48301 caagcaattg ttgaccaagg caaataccga acaccggttt tttaccgata aattttttga
    48361 tttcagggat tacataatca acggcttccg gatcgccggg cccattggat aaaaatattc
    48421 cgtccggatt acttgccaaa atttcatccg cttttgtttt ggaaggaaat acttcgacca
    48481 cacagccgcg ttcgacaaat tttctcaaaa tattttgttt gacgccaaaa tcatataccg
    48541 cgattcgata cggatgggtt cccgtaatat tttcacggaa ctgccacatt tccgcattcg
    48601 gttcagacca ttgatattta gttttgcaag ttacggcatc cacatggtta gcaccttcca
    48661 tccgcggcgc atttttcact ttttccaaca gccgttgcac gcttgtgtcc gtcgtcgaaa
    48721 taatcgcacg catcgcgccg aagttgcgga tatggcgcgt gatagcgcgg gtatccaaac
    48781 cctcgatacc catgataccg ttttttttga gcagatcgga taacgagtgg gatgagcgcc
    48841 aattggacgg ataatcgcaa acttctttga cgatcaacgc tttggcttgc ggtccgcgcg
    48901 attcgaaatc ctcgtcgctg atcccataat taccgatcaa agggtacgtc atgacgatca
    48961 tttggccgta atacgacggg tcggagacga tttcctgata acccgtcatg ctggtattaa
    49021 atacgatttc gccggtcgcc tcgccatccg caccgaaaga ccacgcctca taaaatgtgc
    49081 cgtcttccag cacaagcaat gctttgcttt tgtttgaatt caccttatat cctctgtaat
    49141 attttttttg caatgttggg gtaatcggta atcaacccgt ccacaccgag atcgatcatg
    49201 cgttgcattt ctttttcatc attgaccgtc cagggatata ccgcacaacg cgcttcccac
    49261 gctcgctcca tcagcaatgc attgacaccg acggaattgg gatgccatga atctacaaca
    49321 agccctttgg ctaagcgatg attgagattg ggtagccttt tttcataaat aaaacccatg
    49381 cgcatgtcgg gatcaatacg ctggcatttt ttgacgacaa ggggattgaa tgacgaaata
    49441 accacttgtt tgcgcatgtg catattgtaa atcaaatcaa tgacccgctc ttcgattttt
    49501 ccgcgcaagg gcatttcgct tttgatttcg atatttacaa aaacaggttt accttgaaaa
    49561 accgtcaaca cttccttcaa cgtagggatg cctgtttgaa cacggtccga tcgcaacgta
    49621 agttttttta actcttccaa tgttttggtt cgcaccatgc cgcgtcctgt ggtcgtaagc
    49681 cggtcaaggc gaatatcatg aaacaccacc aattctttat cgcgtgtcag gcgtacatcc
    49741 agttcgacac cgtcataccc ttcatccacg acagcctgaa aacccggtaa cgaattttcc
    49801 ggaaagccgg aatacgtcac accgcggtgc gccatgatca taatatgctt cggagcgatc
    49861 acgtgttttt gccttcgatg actttttggt aataatctat atactgttgc acgacgcgat
    49921 ccgttccgaa attatccacg acccgttggc gtgcgttggc gcgaaatcgc tgatacacgt
    49981 cctgatccgt aagtatttta actacatccg cagccatcgc atcgacatcg ccgatttttt
    50041 cgagatatcc ggtttcgttg tgaatattca cttccgaaag tccgccggca ttggaggtga
    50101 cacagggtac acccgccgcc atagcttcca gtgcggccaa accaaaactt tcttcttcgc
    50161 ttggtaaaag aaaaacatgc gcacaacaaa tcaattccgc aaccgcttct tgtttaccca
    50221 ggaaaacgac atgctcatcc aatccgaaat cttgaaccag ctgttcgcac atgcgccgct
    50281 ccggaccgtc accgatcaag agcaacttgg cattaatgtt ttctcttact ttggcgaaaa
    50341 ttttcaccac atcgccgaca cgcttgaccg gacgaaaatt actgatgtgc atgatcagtt
    50401 tttcgtgatt gggcgtgatg cggctcaaaa aaccatgtga actgtcttcg ggtttatcgt
    50461 ggggacgata tttttccgta tcgacaaaat tgggtattac ttcgattttt cggttgggct
    50521 taaattcgcc gtacgtgcgg ttacgcagat attttgacac cgacgtagta ccgtcgcttt
    50581 cgtcaatact gaacttaatc agatttaaaa acgaacgatc catgcccagc aatgtaatat
    50641 ccgttccgtg caacgtcgta acggttttta agtctttgat gcgattgttt tttcggatga
    50701 tctgttttgc cagatacgcg cttggcgcat gcggtatggc ataatgtaca tgcagtaaat
    50761 ccaaatcctc aaattccgcg acttcagcca tacgtgccgc caatgaaagc gaatacggcg
    50821 aatgatcgaa taccggataa ttcatcactt cgacggcatg aaaataaatg cggtcacgaa
    50881 acgtattgag acgaaaagga atatcggagg tgataaaatg aatatcatgg ccgcgcttgg
    50941 caagttcgat gcccaactcg gtcgctacga caccgctgcc gccatacgtc gggtaacacg
    51001 ttacgcctat tttcatataa tcaatctacc gttttctaaa aaaaacatat acgacgtata
    51061 acacaacgat cactggcaga ccatactgat tgatttccgg tgacgcccac tgaaacaggt
    51121 taagcacgat caaaagaagg agcgctccga tgaccgacca accaaaaatc tgccacatac
    51181 gtttgcgacg cgtatcggat gcggcttcgc cggatgtttc ttcatcttgt atatccggtt
    51241 gtgacgtatc ttcattcata atttatcggc tgattttgat gattttgagt tcgagttcgc
    51301 cggccggaat tttgactttg gccacatcgc cgactttttt tccgagcaat cctttggcaa
    51361 taggcgtatt caccgatagt ttgttatttt cccaatcggc ttcattatcc ggaactaacg
    51421 tataagtcag tttttctttg gttttaacat tttgcaaatc aacggtcgca aggatatata
    51481 ctttgtcagt gggaatttcg tcttctttga ctatgacggc attcatcact ttcatttcca
    51541 tttcattgat tttgctttgc aataactgca gctcttcttt ggccgcatga tattccgcat
    51601 tttctttgag atccccgtgc tcccgcgcca cctggacacg gttggcaatt tcgggacgtt
    51661 taacattttt aagatgttga atctcttctt tgagctttaa taaaccctcg tgggtcacat
    51721 agaacttgga cattcgctga cagactcctt ttcgttacgc taaaataggg aatatggatg
    51781 attgcatcgg tgtgccggtc accaaaacat cttttttgtc tccgacatac acattcactt
    51841 cggtacgcac gccgaagtcg cctacaaaat aaattccggg ttcgatcgaa aaacatgtac
    51901 gcggaataat gcggcgatta tctttggttt caagattatc aatattggcg ccgttgccat
    51961 gcacttcttc accgatcgag tggccggtac ggtgaatgaa atattctcca taaccatgct
    52021 ggacgatatg cgcacggcaa acatcatcga ttttccaacc cggaagcgga cgatcggcgg
    52081 cgatttcttc ttttataaaa gcgatcgcgg catcgcgtgc gccgacaact acatcaaaca
    52141 ctttggcgaa tttttcgggt accgttttac cgacaaaacc catctgcgta taatcagcat
    52201 aaacgccgcg cggatgattt ttcttggccc accaatcaat gagcaccaaa tccccttcat
    52261 ggatttcttt acaatgatct tttgtcggct cataatgcgg atcgccggca ttttcattga
    52321 cagagcaatt tggcaagtcg cccgtggtca acccgcgttc gataaattgg ttgtgaataa
    52381 attgctgtac atcgtattcg gtgacttttt ggcgaccggt tattttttca cgaataaacc
    52441 cccacacttg cggaatgata tccgtcaagg ctttaccggc ttcgacgtgc aattgcaatt
    52501 gctgatcatc ccaagtcgaa tcaaaatatt ggaccagatc ggcggaagaa acgatttcgg
    52561 cttgcgtggt ttttctgacc aattcgattg taccggcatc cacgatagcc acatacggaa
    52621 tgttacattc gggcgaatat tccatcgcaa tttttttatg cccggcggtg atggccttta
    52681 atccggcttc aagatcctgc caactggaaa agatcacttt ttcacccggc aacgtatcca
    52741 aaacataacg ctctatgccg tgtacaagtt ttttgggtgt accttgcgca ggaatgtaat
    52801 aaaaaaatcg gcgcttttgc gtcaaatgtt ccggtatgct cagaatgcta gatgcgactg
    52861 gattgttttt tctaaaactg tacagcagcc acccgtcgta cccgttacgc gcaatatctt
    52921 tttgtattct ctcgatgcgc tcattgatgt taccagttga ttgtgccata atatcctcgt
    52981 taaaaatgtc gaaatcccgc cggttctaag gttttacgat taccggatgc atcggcgatc
    53041 actaccgatg tgccggcgac gatcgtaccg atggcatgta tgttgttttt taatattttc
    53101 tttgctttga caaattcctt aggagacatg gtaagtaata actcataatc ttcgccgcca
    53161 tgcaatgccc acatcataga atctattttc agcacatcgc ttaatttttt tatttccgcg
    53221 tgtaccggga tgcatgcggc ttcgattaac gcaccacaac ggctggcccg gcatacatgc
    53281 gccaaatcgg atgataatcc gtcgctaata tctatcgcgg catgcacacg aatatttttt
    53341 cttctcagta tttgcgccca ttccagtcgc gcatccggtc tgcagtgccg ttgccaggca
    53401 tacgccggtt ttttctttaa acgatcataa aacatcaacc cggccgccga tcctccaaga
    53461 tcgccggtta ccgcgatcac gtccccgata cgggcatcgc tgcgaagcca tggtctattt
    53521 ctttcagcaa cgtttccgat taaagtaacc gttatcgaaa acgcagaact gcgcgaaatg
    53581 tttccgccga caatttgtac attataattc cggcatgctt ttgttatccc gcgatacaac
    53641 gcatgtatat ttttagtacc cgtctgcggg tccatttgaa gcgcgatcac tgcaaatgaa
    53701 ggatctgcgc ccatggccgc gatatcactg acattgactg aaatactttt atagccaata
    53761 tcttcaaacg ccatatatcg ccggtcaaaa tgaatgcctt cgaccattgc atcggtcgtg
    53821 ataacttgtt gttttgccga tgaccgcacc catgccgcat catcgcctat gccgcaaaga
    53881 acattttttc cggtcggata acccgactcc agaatcgcgt gtaactgacg aatcaggcta
    53941 aattcgtcgc ctgttttttt tgatttcatc aggcggcaat atacgggtag aggcttttta
    54001 tagcaattaa ttttatgcgt tttaaagtaa taaaaccgat taaaaatcaa tccgtagaaa
    54061 gtcgtaaatt cccataaaaa aacgcgggct cgcctgaaat gatgggtgag cccgcgtacg
    54121 tattctcgag atgggatccg agttcagtaa aaattaatca aggatatagt ctatcgtgcc
    54181 gataagttgt tcaaactcct tactgtattt gtccatcgtg gtttgagggc cgaccgcttt
    54241 gagaaaccaa ggaccattgg gcgcctcgac gatagccgcc cacatcgcgt aagccggcgt
    54301 ttcatccacc gggccgccca tctgaccggc cacacgcggt ttcatataat taccactcac
    54361 acgaacaacc gttattttga gtttgttgtt ttctgtattt ttgattacgg ccttactttg
    54421 agtatcaccg ccgtccggtt gtttaaactg cccgtaccaa cgtgaaagat tggcatcaac
    54481 ggatccgccg gcgccgggaa aataagacaa tacaagttcc gcatcatctt ccggtttact
    54541 gccgggaaga cggtactgct ccttacgcat cgaattggtc ggcgtttcag acttccaaac
    54601 ttcaggggct ttaaaaacta catggcccgg tgcattcata tcaatatttg catgcggatt
    54661 tccggcatgg ggattacctg cgggcgcatt ttcgggagcc ggtttcggcc cgggtttgca
    54721 actcactatc gtcattacga atacaaaaaa aatcactatg tacttcataa gctaactcca
    54781 aaatcatttt tctttgtgtg cattgagagt ataaacaaaa ttgaggattg gaaacaagta
    54841 ttatcgttat tcacttgttt tacgataacg aatgacggcc aatgtaaaca tggtgacagc
    54901 taaaactatc agcgcggaaa cataaggaat tatatcatgt agcggcgcgc ctttgagcaa
    54961 cacctgccgc agtatgcgaa taaacagcgc taccggattg atccacatgg tctgttgcgc
    55021 ccataacggc atgctgtcaa gcggcgtaaa aagtcctccc atcaacataa aaatcaccat
    55081 aaaaaaccat gcgataaaca tcgcctgctg ctgcgtttct gtaaccgtcg aaataaaaag
    55141 acccaacccc aaaaccaata tcagataaat aatcgtgatg ccaaaaacca acggcaatcc
    55201 gccgagcatc ggtaaatcaa acactaagcg gccaatagcc aatcccatag ccaattcaaa
    55261 taatccgatg atgagaaacg gcaacagttt tcctatgatg aattgatgct tttttatcgg
    55321 tgttacgttg atttgttcga tcgtaccgat ttctttttcc cgcacaacat tcatgcctga
    55381 caaaaacaga ccgatcatcg ttaccaatgc cacaagtatg ccgggtatca tataatgttt
    55441 ataattcaat tccggattat accaatttct ggcgattatt tcgatgcgct gcgtcatgcc
    55501 ctgtgtctgc tggaaaggca cgacttgtct ggtaaaatcc tgaatgatct gagccgcata
    55561 tgactgaatc acgccggccg tacttccatc ttcggaattg atcactaatt gcaatttcac
    55621 cgtcgctccc gtgatcacat ctttttcgaa aaaaactggt atttcaacga tcaaactggc
    55681 ttcgtttttt aaaacagatt cttccgcttg cgcataatgg gtcggcatgt ccgtgagaat
    55741 aaaatgatgc gttgacgtaa aacgctcaat cagttgcctt gacaaatccg accggtcgcg
    55801 atcaacgatt tgaaatttca cattcttcac atcaaatgtc gccgcattgg aaagtatcaa
    55861 cagctgaatg aaaggcataa cgaaaataat aggcaacatg cctttgtttc gaaaaatctg
    55921 aagaaactct ttacgcaaga taaatcgaat cgtccgcatc gtcactccaa tcggatttta
    55981 aattttcgcg cactgatggt catgagtaaa agtgtcatac ccgcgagcac cagagtttct
    56041 ttccacagat attcgatacc aacgcctttg agcatgatac ctttgacgat aatgagaaac
    56101 catttagccg gaacaatatt gctaaatatc tgaagcggca ccggcatact ggaaaccgga
    56161 aaaataaacc cggaaagaat aataacgggc agcataagtc cggccaacga tgccatcata
    56221 gctacctgtt gtgtacgggc aatcgtagaa atcagtaacc caagcgataa agccgtcatg
    56281 ataaaaagta acgcctccgc gtaaaaaagc gcgtaactgc cgcgaaacgg cacttcgaaa
    56341 acaaatcgtg ccagtaaaag aaccgttgac gtattgacca aggacaacaa caaataaggc
    56401 aatacttttc cgacgatgat ctgtatcggc cgcagcgggg aaaccaataa aatctccatc
    56461 gtcccaagtt ccttttcgcg cgtgatggtg atagaagtca tcagtgcgga tatcagcatc
    56521 agcaagagcg caataagccc cgggacaaaa agatatacgc tacgcatctc cggattgaaa
    56581 agcattttca cttccgtgac tatcggcgtc ggggcatgcg gattaagatt cgccgaataa
    56641 tcacggataa tggaagtcgc gtaggctgtc agcgtcgtag ccatattggg ttccgtcgca
    56701 tcggtgatca gttgtatatg cgctacacct tcgtgataca aacgcgcggt aaattgcggc
    56761 tcaaaaacca cgaccatctt tacgtcgccg gtttcaaatg ccggcgctat ttgatcatcg
    56821 tgcgtcagtg tttgaacatt ttgaaaataa ccggatgata aaattttttg agtgacttcc
    56881 tgtgaacgcg catcgtgggc atgatccagt atggcaatgc gcgcatcatt gatttcatta
    56941 cgaatggcaa agccgaataa aatcatttga atgacgggca taccaaaaag aatcaatacc
    57001 gttcgtttgt cgcgaaggat gtgaaagaat tctttgacga taaatgcacg aaagcttttc
    57061 ataattcttt actttgctgc ggtcttgcaa ggcggataaa cacgtcgttc atagattgcg
    57121 cctcaaacgt ttgcttcaat ccgcgcggcg tgtccagcgc agccatgcgc ccgtccacca
    57181 tgatcgacac gcgatcacag tactccgctt catccatata gtgcgtggtt acaaatacgg
    57241 tgacaccgcg atgcgccgct tcgtagatca tatcccaaaa ctgacgccgt gtgatcggat
    57301 ccacaccgcc ggtcggttcg tctaaaaaaa cgatggccgg atcgtgtaat aatgccaccg
    57361 agaaagccag cttttgtttc catcccaaag gcaactcgcg cagcatagtc cgggctacat
    57421 catgcatgcg cagacgctcc agtagcattt cgcttttggt acgtatcgcc gcgtcggata
    57481 aaccgtaaat accgccgtaa aaggtgatat tttcttgcac ggtcatatct tcatacaacg
    57541 aaaaccgttg actcatgtag ccgatgtttt ttttaatgct ctccgtttgt gtataaacat
    57601 catacccggc gacttgtgcc gtacccgacg taggcgccaa taatccgatg atcattcgca
    57661 tcgcggtggt tttaccggcc ccattggcac ccaaaaaccc gaaaatttca ccgcgaccga
    57721 catcaaacgt aatcgcattg acagcgataa acgaaccaaa gcgtttggtc aaatcacgaa
    57781 ccgtaattgc cgcatcggat ttattgggtt tcatgttgct ccatcaatgc cataaacgaa
    57841 tcttcgatgg tcggttcggt gcgcatcact tcgacatcat caaatccctc cgtcctcagt
    57901 tcatcacgta attgctgttc ggaaaccccg ggacgccgat ccgtgtaatg caaaaattcg
    57961 ccgaacggaa aaatagattg tgtttccgca gatccgcgca aatgctccat caaaagatag
    58021 gtcttacgac ttttgatcgc atacagcggc gaacgaaatt ttcccaccac gtcggccggt
    58081 gtgtcgatat ccaaaatttt accgctttga atcaatgcga tacgatcaca aagcccggct
    58141 tcatccatgt aaggcgtgga tacgatgatg gtaatacctt tggtctggag tcgtttgagc
    58201 atgtgccaaa actcttgcct tgaaacagcg tcaacacccg tggtcggttc atccaaaaaa
    58261 agaacttccg gcttgtgtat caatgcgcaa ctcaaggcca gtttttgttt cataccgccg
    58321 gacaactggc ccgcgcggcg ttttttgaac ggttcgatct gaacatagat gtcgcgaatg
    58381 agatcgtagt tttcctgaat agaggtgcca aaaaccgatg caaaaaattg gagattttct
    58441 tctacactga gatcctgata caacgaaaaa cgacccggca tataacctaa tcgtttgcgc
    58501 aattccctga agtcgcgaac aacgtccaat ccgctcacgg tggcgcggcc ttcgtcgggt
    58561 atcatcagtg ttgtcagaat gcgaaataac gtactttttc cggcgccatc cggtccgata
    58621 aaaccaaaaa gttctccggc tttgacctga aaactcaggt ttgtaatcgc tgacgttttg
    58681 ccaaatcgtt ttgtgagatg atccgcttcg accgatatca tagtttggta ttatccgctt
    58741 ggtttttcac aaaaacataa tacccgatca gagaaataat gccccacact atccatcgga
    58801 agcccatcgc agcaactgtt cttttttcgt aggccaaacc cgatgcgata tgtattccca
    58861 ataaaacaaa aatcacactc gtcgtaataa agatgtagat gcttagtttg taggctacgc
    58921 gcggatcaat ccacacatac agacttgctg cgacatagat aaaacctgaa aaaaaattaa
    58981 accagacaac ccaaggtatg actgttccga aaacaccctc attaaaaaaa atggcagcgc
    59041 cgcccgccgt gatggtcgcc agtccaaaga tggtcgccga caacgaaata agtaatttta
    59101 ttattaacgg tttcatttta aaaaacttct aatctttcac ctgacttcat tgaaaataca
    59161 tttcgcccgg catcccgatt ttcaaactgc catcgttttg aacttttact ttgatggcgt
    59221 acaccatatt gacgcgttcc tctttggtct gaatgatctt cggtgtaaat tccgccttag
    59281 aggcaatcca aatcacttcg ccttcgagcg agcgattgtc cgtagcgttg tcatctatcg
    59341 cgacgcgcac tttttgacct atttttacat gcgacagctg atcaccgctg atgtacgcgc
    59401 gcaaatgcat cacacggatg tcggcaattt tataaagcgg tttgccaaag gatacgatct
    59461 catacgattg tacatattta gccaatacgg ttcccgatat cggattgata atgagacttt
    59521 tttgtatttg atcgttgatc tgacgtatct gcgcatccaa cccggccagt tccgacagta
    59581 cgccggcatt ttgtgcttcc gtggaagcga tttgtttttc gatcacacgc acctggcctg
    59641 tgatgtcatc catttgtttc tgcgtcgcta ctttttctcg atagagattc tcaatacgcg
    59701 ttttttccgt cattgccaca cggcgctgct cctgcagtac ttccatttgc gcaaggacgt
    59761 tagctgatcg ggcgtctaca ctttgtttgg acgcgaccaa ttgtatccgc ttcagcgcca
    59821 gttgtgtggt atcaatacat ccgacgcgtt gttgttcact caaagtgacc ccttcttcga
    59881 catcaaaaaa aagcagcttt ccgccggctt cagcagcaat caaagtttcg actgcttcaa
    59941 aattaccgta cgcatcggcc gtgttgtttt ttttggaaca cgccgtcagg gttattaaaa
    60001 ataatgtgat cgttataaag gttttcacat gcatctccta aacccgtaaa ccattgagca
    60061 ccagcatggt gacctgcgct ttacggcgtt ctataaattc attgaattgt ttttcgtcca
    60121 tactgaaaaa aaactcgacc atcggtttgg caacaaaagg aaaaacacat tggcttaaaa
    60181 tactaagaag caattgctcc ggctcgatcg ggatataacg tccatcttcg atgccttcgc
    60241 gtatttgacg tttgatgaca tcgagcttag gtactatacc gcgttcctca aatatgcgcc
    60301 gcatgcgctc gggattggtc gtcatttcat gtaaaacgaa accgggaatg aagcgattgt
    60361 tttttataaa atcgatatag gtgtggatca gtttttcgat tttttgctcc agtggtgctt
    60421 cggaattgat catatgtaaa acgagaggaa aaaaacgtga gatcgcctct tcaaaaacag
    60481 cttgaaacaa acggtctttg ctgcggtaat aataatgcaa aagcgcttta ttgatgccgg
    60541 cttcgtctgc tatttcctgc atccgggcgc cgtcaaagcc tcgacgatga aatatggaac
    60601 gagcggcttt gatgatcttg ttttctgctg aatcttgttc tgtttttatg ttttgatcca
    60661 ttttaaatat tgataataat aaacttaaat tgttaattaa tagtttaatt aaatagttta
    60721 actaaacagt taaatatata ttataaacac agctgtgtca agttttttat gtagcaaacg
    60781 ctaaaaaact gtctttgatg tttatgttta gtaaaacaat ttagaaaatt cgatgaatcg
    60841 gtcttacaag tataaaacat aaatttttat ggaaaatgag ggatataaat tattataata
    60901 cccctatcca aaccacttta aaacttctca tgacaaaata cgtttggcgg agcctgcttt
    60961 tttttgaaat agccctaata tcgctgattt gcagtgtaac ggcacaggta cgttttgttg
    61021 atctggacag gctttccctc gaagaagggc tctctcaaag ctccgctatc tgcatgctac
    61081 aagacaggca aggttttctt tggattggca ccgaagacgg acttaatcgc tacgatggat
    61141 attccattaa gattttcaaa gccgatccgg cagattccgg tgcgttacaa aatgcttgga
    61201 taacttcact ttttgaagat cataccggca atttatgggt aggaacccgc ggcgggctgc
    61261 accgctacga tgcggcaaaa aacaggttta tttctatacc gctggataat acatcattgt
    61321 ccgtctttgc aattacggaa gatttgcaac accatctttg ggttggcaca cagcaggccg
    61381 ggttattcag aaaatctgta tcaacggccg attggcgtca gtatcgtttt gaccccgcgg
    61441 atccccggtc tgtgagcagc aatgatattt ttgttttata cacggatgat atcggacgtg
    61501 tctggattgg aacacgggac aatggtctca atcgctacga tgcgatggcg gataatttta
    61561 ttcgttacca acccactaca ggaaatccat atagcataag cggtttatcc attcgcagca
    61621 tccgtcagta taaaacggaa atctggattg gcacactcaa taacggagtc aatcgtcttg
    61681 atccgctcac cggacgattc aattcgtatc aacctgatgc ttccaaaccc ggttccataa
    61741 ctcataacag catttggtcc ttatttgcgg acagtaaaga cattttgtgg ataggtacat
    61801 ttggcaatgg tgttatgcaa tatgatcctg caggagatac ctttgtccaa attcagccca
    61861 aaccttcctc gacaccgcgc aatgatcaag tcgtagtttc tattctggaa gaccgcagcg
    61921 gcatgatgtg gtttggcact atcggtgatg ggttgcgcaa ataccttcgt atcaaagacc
    61981 gctttcaatt tatcaacgca caccccaatc ctaaacgcgg tttaccggat aatatgatct
    62041 ggtcactgct cgaagatcgg caatccaata tatggatcag tactcaaaaa ggcatcacgg
    62101 tctataaccc taaaaaagat gttttttctc aatataccaa cccgcgaagg gaagcgtgga
    62161 aactcggcga aacagcctac gcaatggcac aggacagcaa tggtgtgatt tggtttgcga
    62221 cgttcggaga cggtctcaaa cggtttgatc cgcgcacaca gcgcatttca gcgtttcaag
    62281 ttaataaatt ttcgccgggc ggaattccta caaataatat tttagcgcta tacgttgacc
    62341 acgaaaacgt actttgggtt ggtacgtacg atgcagggct tctccgattg gaacccggaa
    62401 gcagaacttt caaagttttt aagaaaaaag aaaaagattc aacatcgctg caagacaatc
    62461 aggtgcgtac cgtttttgaa gacgcgcgcg gcgttttatg ggtaggcacg gcgcaaggcg
    62521 ggctacatcg tttcgacaga aattcgtcta cgtttaaagt atattcgacg caaagtccgg
    62581 gactgaaacg tcttcaaaat aattttgtca gcgccgtatc cgaagacaaa gacggtactc
    62641 tgtgggtggc cacctacgga ggattgcacc ggatgaaccg cgagacggaa acatttattt
    62701 gttactccga aaaaaacgga ctcgcaagta accaaatata cgcattgatc aaagatcagc
    62761 gcggtcgttt ttggatgacc accagccacg gtttatccgt actggatccg cacgatgccg
    62821 tacctgtatt tcgaaactac acatcgcacc acggcttgca aagtgatgag tttaatatgg
    62881 gcgctgcgat tcgcaatgta tccggaacga tcatggcggg cggtgtgagc ggcgtggtga
    62941 tttttcatcc ggatagcctt gaaagtaaaa aatacgatgt cccggtggct atcacggccg
    63001 tccgtgttgc caatcgcatg ttgggatacg aaccttggga tgacaaatat acgacgcttc
    63061 ggcatgatca atctaccttg gcttttgagt ttgcatcgtt tgatttttct tttccagaac
    63121 agaataaata cgcctatcgc cttgaaggat ttgaaacgga gtggtccgat gcggatgcat
    63181 cccgacgtac ggtcaattat accaatcttg atcccggaaa atatgttttt caggtacgcg
    63241 gcaccaatag cgacggccat tggagtgata aaacggccgc atttcgtttt gaaattctgc
    63301 cgccatttta taaaacatgg tggttcgtcg gcagcagtat ttttctgatc gtgtttttag
    63361 ctattctatt ttatcgaata cgcatccgcc gcatcgaaaa tcaaaaaaac gaattggctc
    63421 gccagataac ggaacgcaca agagagttgc gtgaaaaaaa cagcgaattg gaacgcgtca
    63481 atgcgatacg aaacgaactt ttaggcatgg ccgtacatga cttgcgtagt cccatcgctg
    63541 cgatacgaaa tctggttcag tttcacttgg aagctttaaa aaatcaaatt aatgatcgtg
    63601 aaacagatat gcatgatatg gaacgagcgc taagtttatc cgatcgcatc agctcgacat
    63661 taaatgatct gttggatatt tccgctatcg aatcgggcaa attatccata accaaactcc
    63721 ccgagaatat cgaaaccatt attcatgatg cgttcgagct caatcttcgt gcagccgagc
    63781 gcaaaggtac tacgttaaaa tccgatatcg cacccaacct tcccaaaatt cttgtggatc
    63841 gcattcggat cgccgacgta ctcaacaacc tcatcagtaa cgccgtcaaa tacacaccta
    63901 aaaacggtcg catccgtgtt tatgccgaaa tatcgggcat gtttatgcac atccatgtcg
    63961 aagacaatgg ccagggctta acacaagaag atttatccag cgctttcaaa acgttcacgc
    64021 gattgagcgc cacaccgacc gatggtgaaa ccagcaccgg gttagggctg gccatcgtaa
    64081 aaaaaatagt cgaactccac ggcggctcag tttgggtgcg ttctgaaaaa aataaaggct
    64141 ccacatttag tttttccgtt tcgctcgaag gacaccatta atccgctcag gacgatcctt
    64201 ttttctgtgc gccgcgttac tttgaaatcc tccttgcaat ccatttcggt tttttctaat
    64261 atggcgcgtt ccaagtttca aaaataattg aaactttaaa tgaaagcgaa tttatgctgc
    64321 agaagatttt tgataaaatc gaaaacaacg aacgcctgac gtttgaagac gggatgacgc
    64381 tgtacgcttg caaagaactg ccgctgctcg gtttcctcgc caaccatatt cgccgaaaaa
    64441 agcggccggg taatgaagtc acgtatatca ttgatcgtaa tattaactat accaatgttt
    64501 gcgttgatct gtgcagtttt tgtgcctttt atcgcccgat aggtcacgac gaaggttacg
    64561 tccttcctac ggaagttatt gatgaaaaaa ttgacgaact tatcgcactg gaaggcattc
    64621 agatccttat gcaaggcgga ctgaatccaa aactcaaaat cacttattac gaagatttat
    64681 ttcgtcatct gcgctccaaa taccctcaga tttggctgca ctgcctgagc gccccggaaa
    64741 tccgctacat agccaaagtt tcgggtctta cactggatga aacggttgcg cggctcaaag
    64801 ccgccggact tcagtctgtg cccggtgccg gcgcagagat ccttgtagat cgtgttcgct
    64861 cggaaatttc acgcggaaaa tgcgacaccc aagagtggct ggatgcccac agtatatttc
    64921 acaaacacgg cttgacatcc acggcgacga tgatgtacgg tcatgtggaa acccgcgaag
    64981 aacgcatcga acatattcta aaaatccgcg atcttcagga taacagtttg aaaaactacg
    65041 gtctcggttt caccgcattt atcccgtgga atttccagcc ggataatgtg cctctcggca
    65101 aaaaccaaaa cctacgtacc accacgacag tagagtatct ccagacatta gccatttctc
    65161 gccttttgct tgataatatt gataattttc aggtgtcttg ggttacacaa gggcaagaca
    65221 tcggccaatt gggtctcaat tatggcgcca acgatttcgg cagtctcatg atcgaagaaa
    65281 atgttgtaag ccaaaacggt acctctttta acgtaacggt aaaagacata gaaaaaatga
    65341 ttcgtgatgc cggttatgaa cccaaacgcc gaaataatca atacactttt gttaattgat
    65401 tgagcatagc ctcttcgcaa tagcatgtca cgtaacaaac ggatcgcttt tgatttgcaa
    65461 attaatttaa aaccggattc acggtttatt tcctttctat gacaaaactc tcatccaaag
    65521 atgtcctccg catattagac ttacaaccgc accccgaagg cggcgcttat cgcgaaactt
    65581 accgttcaaa aaatccggca aaagtggatc cgcaacgatc ggtatgcacc gcgatttatt
    65641 ttttagtaca agcacatcaa aaaacagaat ggcatcgtgt caaaaatgac gagatgtatc
    65701 atcattacgc cggcgcaaca ctggacgtcc tcctcgttag ccccgacggt gtttttgaaa
    65761 aacataaact tggcgatgat ttagagacag gtgcgcgacc gcaactttta atccccggtg
    65821 gttattggca atcggtacaa gcgcatgatt cttgggtttt ggtaggatgt acggtctctc
    65881 cgggattcga tttttcagat ttcgaattgt ccaacgaagc cgctttaaaa aagcaattcc
    65941 ctcacctatc cttttgaaag gtacacgtat gaccacgcag gaagttttta tcaacaaaaa
    66001 ttatcagatg cgtgcaggat ggcgcgtact tattttcatt gtcgcattta tcctgatcag
    66061 catgccatcc gtttatctgg gattattatt tactaagttt cttgagttcc ccaaagaata
    66121 caatgaaatt atcgtaaaat ttttaaccac tattatccct ttaggtttat catttttcct
    66181 cttgaaaaaa ctcgataacc gctctaccga ttatcttgga ttaaaaacgg actgtgccgg
    66241 atttatagag ttggcgtggg gactccttat cggttttgta atgttgacga tagcggttct
    66301 aataccgtac gcgatgggct atgtgtcgct tcagttcgcg acggaaccgc cttcgtactt
    66361 ctttagcggt ctggcattca atcttttact tttcatcgtc gtaggtttta acgaagaaat
    66421 tttattccgc ggttatatct ttcaggctac cggtgaggga cttgggttcg tcggcgccac
    66481 gctgctttat tctctgcttt ttggggctgc tcatctcgga aatccgaatg tttctctatt
    66541 tggcattatt aatatcgtgt tggccggagt cttattatcg ctcgcgtata tcaaaacacg
    66601 cgcgctttgg atgccgatcg gcattcacat tgcgtggaat tttacgcagg gatatatttg
    66661 gggattaccg gtgagcggga cgacggtcat tcaaccaatg atcatcgctc aggagatcgg
    66721 accggacgaa atcaccggcg gaacatttgg tcctgaaggc ggcatcatgt gctcgatcgt
    66781 atgcatcggc gcatgtttat tgatatggaa atatttttcc ccatccgatg cgatgaagca
    66841 ggtcatacaa gaggccgaga ccgctcaacc attcaaaccc aatatcagcg aatcaatcgg
    66901 cagtaatact aacccgaata acgggatttc ataaatattt tcaagaaaac tatccccaaa
    66961 aaccgcagat ggggcgactc accaccgtat tggcgttaga tttataggga attttttgtt
    67021 ttaccgttaa aaacaaaatg tacccataaa ctaacttaaa gaaaggtgga tatgaatcgg
    67081 aatatctttt ttttgggagt gttatttttt tcgtcttttc tttttgcaca tgacggagat
    67141 atacacaaag attcttccag tacgaccgtt cgtacgataa ccaatattgc tcccaatatc
    67201 tacatgatac gccatccgga cgccccggac gggtttcctc aaggcaatac cacggttatc
    67261 atcggtgatc gcgaagtatt ggtagtggat gcctgctact tgccttcttc agccaaacaa
    67321 gatatcgaac aaattcaaaa atggacttcc aaaccggtac gttatctcgt taatacacac
    67381 tggcactacg atcacacaat gggcaacggg acatacgccg aggcgtttcc gggaatttcc
    67441 atcatcgcgc atcacgagac caaaagaatc attcagggtt ataacccggg ttggtttgcc
    67501 cgttttccca atcgttcagc gatttttaaa agtgccatcg aatccggaaa agacgccaat
    67561 ggtaacaaat tatccgaaag tgatatacgc gactacaaaa agtatcttga cggattgaca
    67621 ccggtacaag aggaatttag taaaatcaaa gatcgcgcac ccaatctttc gtttgatcat
    67681 gaattgacaa tagatctcgg taatcgtgaa gtgcaaataa aacacctggg gcgtggaaat
    67741 tcagccggcg atataatcct ttatttaccc aaagaaaaaa tcatcgtcat cggcgatttg
    67801 atggatcacc ctgtaccata tcttggcagc ggtttccccg gtgaacaagt cgaaacactc
    67861 aacaaaatag cgcagcttga tgcagataca ttcataccgg gccacggaaa tgttctcacc
    67921 ggaaaatcgt atcttttctt attacgagac ttcctcgccg aagtcgtagc ggcggtgcgt
    67981 tctgaaatcg aaaaagtagg aaataatccc cgattgattg atcaggttcg agagtcggtt
    68041 cacaaacggg ttgattttac ggcatggaaa aagagatttg tcggtgacaa taaagaagac
    68101 ggcgattttt ttgacagctt ctctgttcaa ggcgttttta actgcgcttt caatgagctt
    68161 gtaagaaaat aatttttcgc aatagtctta taaaccggct tattcatact ccgtagaggt
    68221 tatgcggtta agataataaa atctggacat ttcatgttca tggttatctg acagtcttta
    68281 gcataacctc tattgttata aaattcttcc ctcactatct accacgcata ttcataattg
    68341 agccgtcatt ttaagggtcg cacccttagt tatggtgatt taatagatac cgaaatcacg
    68401 taatttacgc aaactattgt aaaatcaaca ctttttcaat tgacttagct atattttttt
    68461 gttatattcg cgggttgcgc taagaggttg atttctatta ttttcaataa tttagcgcgt
    68521 tgcaacaaac tattcatcgt gtttgaggct ttcatgaaac ttgcagattt taagtttaag
    68581 ttaccggaaa aatacatcgc gcagtacccg gtcgaaccgc gggacaaagc caagcttatg
    68641 gttttggatc gtaaaacggg tgatatcgaa gataaaatat ttcgcgatat tacggaacac
    68701 atcacaaaaa acgattgcct cgtcctcaac gaaactcgtg tatttcctgc ccgtttgatg
    68761 gctcgcaaag aaaaaaccaa cgctaaagtc gaagtgtttt tgctccgcga actggaaaac
    68821 aatctgtggg aagtgcttgt caaacccgca cgcaaagtgc gtatcggcaa tcgtcttgta
    68881 gttgatggcg tctattgcga tgttattgat aatacggttt ccggaggccg tgttgttcgc
    68941 ttcatttacc ctcaagccga tttccataaa tttatagaca aagtaggcca gtgccctctg
    69001 ccgccgtaca tcaaacgttc tgccgaagag agcgataaag aagattatca aaccgtattt
    69061 gcacgtgtac gcggctcggt tgcagcaccc acggcaggtt tgcattttac caaagaactg
    69121 ctcggacgcc tgaaacgtaa gggtgttact atcgtaccgc tcgtgttgca cgtcggtctc
    69181 ggtactttcc gtaaagtgca ggtggaagat ttatcacggc acaaaatgga ttcggaatat
    69241 tttgaaatac cggaaattac cgcgcgcgcg atcaatcaaa ccaaagataa aggcggcaaa
    69301 gtgatcgccg tgggttcgac cgtcgtacgc agcatcgaat ccgccgttac ctcgcttaac
    69361 cgcgtaaaac cgcatcgcgg ttggaccgat aagtttatgt atccgccgta tgaatttaaa
    69421 atcgttgata aaatggttac caattttcat acaccggaat ccacactttt gatgctggcc
    69481 gcagcatttg gcgggaaaga taacatcttc aaggcctaca aaaaagcgat gaaagataat
    69541 tatcgtttct acagctttgg cgatgcaatg ctgatcgaat aaaaaaacta catgaagata
    69601 aaaaacccgc ttttgagcgg gtttttttat ttagccaaaa atatactaaa acatcaaacg
    69661 gcaacaacgt actttgcaaa gttatcgaga atcgcttgcc atccgttttt ttgcatatca
    69721 acgggattct cttgttccgc atcaaaaacg ctaacaattt tcgtgctatt gcctgattga
    69781 gaaaaatgca cgcgcacctt ccttccgtct tccatcgtat aagcgatcaa ttcatactgt
    69841 accacttcat cgtacgagcc tgtgaaatca aagccgaaac tgccgtcttt ggcttccata
    69901 cgcgataaaa aacgcccgcc gacacgtaaa tcgttttctg cttttgttgt atgccagtcg
    69961 tccgaggcgg cattccactt aacgatatgt tcaggcgatg tccacactga ccaaactttt
    70021 tctagcgagg cttgtatcgt agcctctatg gtgacttgtg tgtaaggatt ttgcatgaat
    70081 gtgatctttc aaaaagacgt tgtttatctc agttaaaaca tctcagtttg ataaaaagta
    70141 actgcgcttt tacttcttca aaagtttaaa ggcatgcatc catgaattgg atgtaaaacc
    70201 cggaatacac cataatatac agagcggttc tatcgcacgg gcggcttcga ctttgcccat
    70261 gtccgcccaa tcccaaccaa attgcgctaa aatagccgtg acttgtgcct tggcggcatc
    70321 gttattaccg caaatgaaca tggtaggcgt accttccgcc attttgggat tgaccattag
    70381 tgcattacca acacaactaa aagctttgac aaaattggcc tccggtgcaa gaacctgtaa
    70441 ttgctccatc aaagattcat tgggtccggt aaaaaattgc aatacgccgt tgaccggagg
    70501 aacttctttg atcgggttat ttacatcaat cacagttttg ttttttagat tggtgatgcc
    70561 ggcgcttttg actgcatctt cagccgcatg accttttacg gcaagcacga tcagctcacc
    70621 aaatgatgcg gccgtagcca tactccccac ctgagcttta tcgccgtttt tggatttcca
    70681 ttcggataat ttagttacgt cacgcgttcc catcatcact tcatgaccat gcttgataaa
    70741 accactggct aatacctgag ccacttgacc ggagccgagt acacctattt tcatattaat
    70801 ttctcctgtt gttaataaac ttttaatgtc gtttttcatc acaatccgca atttgaatta
    70861 taatcaataa cgcgaaagcg ttttttaaat taattttttc gtacagagtt tacttccatc
    70921 atatttttta ccgtcatatc agatttattt tctccgattt gttgacgcca catggcgtaa
    70981 tacagtccct tctgtgcaag caattcgtca tgtttcccgg cttcccctat gcgacctttt
    71041 tctaatacga atatccggtc ggcatgcatg atggtcgaaa ggcggtgcgc aatacatatc
    71101 gtcatatgtt cctgacggtg tgagatatca cggatcgtat gcgtgatttc ttcttctgtc
    71161 agcgaatcca gactggatgt ggcttcatca aaaaccaaca aacgcgggcg tcgtaacagc
    71221 gcgcgggcaa tagacaagcg ttgtttttcg ccaccagaaa ctttaacgcc gccttcgccg
    71281 atcaccgtat cgaggccttt atcagcacgc gccagcaaac tgccgcatgc agctttattc
    71341 aaaacatcga gacattcctg atccgttgcc ttcgggttga caaacaataa gttctcccgt
    71401 atcgtacccg caaaaagctg tgtatcttgc gtaacaaagc cgatctgctc gcgtagcaca
    71461 tccaaatcga tcgtgtcgcc gccatggccg ttatatcgta cgataccgga ctgcggtttg
    71521 tataaaccga caagaagttt gaccagcgta gttttaccgg cgcccgacgg accgacaaat
    71581 gcgatcgtct cgccgcgatg caccgaaaat gaaattccgt ccaaagcatg gcgctgcgcc
    71641 gactgatgtt tgaacactac attttcaaat tccaattttt ctatatcggc gatcagtacg
    71701 ggattggccg gtttggcttc gcacggtgtg ttgagaatat cttcaaaatt tttgagcgac
    71761 acctcggctt cgcgataaat attgataacg ttgcctaact cctgtagcgg gccaaatatg
    71821 aaaaaggaat aaatgaacaa cgaaaagaat tcaccaaatg taatctcttg ggcgaagatc
    71881 aaatacaaca tgataaataa aatcgaattt ctaagaaaat taaccagggt cccttgtacg
    71941 aaactcaagc tccgtatgta cttcactttt ttgagttcca gcttcagtat ctttttggtt
    72001 gtaccgttga gacgttcgat ttcctgctgt gccaatccca aacttttgac caattcgata
    72061 ttgcgcaagg attccgtcgt tgagcccgcc agtgccgtgg tttctgcgac aatggctttt
    72121 tgcaccactt tgattttttt gctgagcacc gaactcaccc agccgataat aggtatggta
    72181 gaaaaataaa tcggagcgat aacccaatgc acgctgatcg cgtaggccgt cacgaaaata
    72241 attccgacca atgtcgtaaa taaaatattg ataaatgccg agatcaggcg ttcgacatcg
    72301 gttctgactt tttgaagttt acccagtgtt tctccgctgc gctgatcttc aaacgtgcta
    72361 taaggaagct ccagtgaatg ccgcagaccg tcggagtata tttgggcgcc taatttttgg
    72421 gtgatcgtat tgatataata gtcctgaaaa tttttggcaa cacgcgacac aaaagcgacg
    72481 ccgatcgctg cgccgagtaa tacagatacg cctttgacaa actcatcaaa cgaataatct
    72541 ttgtagcgcg tcgcatattc gtcgatcacg tgacgaaaaa tccacggatc aagcaatgaa
    72601 aaaatctgat tgaccgccgc caaaaacaac gccaaagcaa ccaatttcca ataatttttc
    72661 agatacccta tcaacaattt cataatctca tactttctat taactgactt agcaaaatta
    72721 ttctgtaatc atttcgacca gacgacgtat gcgcggaaca gctatgaacg cgacgggaaa
    72781 agcaactaaa aacgaaaaac cccaggcctg aagccataaa gcgacatgcc acgtctccat
    72841 accaaatgtg cgaaatgtca tcaccaggga cataatgcaa ctcaacgtca gcgccataaa
    72901 gaggacgaat aacacttggg aatgtttttt gttaaatttc atacacagaa tccgttttaa
    72961 ttcatcgaaa ataaggtcga acgtactgta gctttattgt gaaaggctaa ttcataaata
    73021 tcgaagcgaa aataatcgta cagtggaaac gtagctaata tgtcaaacgc ttccgaatca
    73081 ctttgtgccc ggatgacaat ccagagtttg gtacgatcgg cagacaaagc ataactttgt
    73141 acaacccgtt cctccaatag ccgattgatc aatgtgcgct gttccggaat tttggagaaa
    73201 aacgcttcat cgggaaattc cggtaaagat acatccacca tatattgatt cactttacgc
    73261 ctcctgcatt atttttaaaa ccgcgccgca ttatccttgg caaatcgttc aaaatctatc
    73321 ggatctttcc cggtcatatc acgcgtcacg tttgaaattt ctgcggcgta ccccgctttg
    73381 ttgatcgcat gaagctctaa taacccgtca atagcccagt tcggcatttg catttctacc
    73441 atcgttttcc gcgccgcttc ttcgggcacg tccgtgtagg aaatggtttt acccgttgct
    73501 tcggaaagta tctttgaaac ctcggtgaca ctcaatgcgt cgggaccggt gatcgtataa
    73561 gctttgccag cgtggccgct gtgagttaat acatgcgctg ctacggccgc aatatcacgg
    73621 acgtccaccc aacttacttt gccttcgccc aacggcaggt atattacatc attttgaggt
    73681 ggaaagaaat tgataaaatt ttgcataaac gaattgggac gcagatgtgt ataaggaatg
    73741 ccgctggctt caatatattt ttcaacttcg cgatgccagc gcaccaaagt aatacccggc
    73801 tcggcatccg ctccggcggc cgataatttg acgatgtact gtactccggc tttttttgct
    73861 gcatccacga tacgtttccc catcgtgacg gattcatcgg taaacggagt aagcataaat
    73921 aattgcttca cgccacggag tgcattttcg atcgaagacg gcaaagtata gtctatgtgt
    73981 accggcgcta catattcgct tgctaaggtt tcagcttttt ttatatcacg gactcccgct
    74041 ttcactttga ctccgagtgt ggcgaggtgt ttgagtaatt gcgaggcagt tgtaccggta
    74101 gctccggcga taaatatcga attcgacatg agtttctcct aataaataga tttatatgtt
    74161 gccaatctat aatgatagta ttaatttaaa tctaaagtaa ccattcgtca agtaagcact
    74221 ataaagtatt atagtatcta aaaggatact attggagtaa ccacatgaaa aaacaattac
    74281 ccttttgccc ggtcaatgca acactcaatg tgatcggagg gcgctggaag ccggtcatac
    74341 tcaaccttct gcgcgaagat acacttcggt ttagcgcgtt gaaacgccaa ataccgggta
    74401 taacgcagaa aatgctcacc cagcaattgc gcgaattaga acaggatggt atcgttcgtc
    74461 gcgaggtgtt ccccgaagta ccgccgagag ttgaatattc gctgaccgaa tttggtcaaa
    74521 gcttaaaacc tatattgaaa gccatgtgca gttggggatc tgaacaccgt cggtatctgg
    74581 atcgtaaatt gaaagaaaat agatccgtcg aagaggccgc ataaatacaa tgaaacaacc
    74641 cacacgacta cataatttca tcatccgcaa ggctaacatt gaggatgtac cactgatctt
    74701 aagttttatc accgaactgg cagaatacga aaaacttcgt catgaagtcg tcgcaacgga
    74761 agctctttta caaaaaaatc ttttcgataa taacccaaaa gctgaagtca ttatcggcga
    74821 attggatgga aaccctgtat gcttcgctct cttttttcac aatttttcga cttttttggg
    74881 taaacccgga atttatttgg aagatctata cgtaagacct gccgtgcgta gccaagggat
    74941 tggccgtatt atgctggctt acctggcaaa gctggctatc gaacgaaact gcggacgact
    75001 ggagtggtgg gtattagatt ggaataaacg ggccatacaa ttctatgaat ctatcggcgc
    75061 tacaccgatg aacgagtgga ctgtgtttcg tgttaccggc aaaaacttgg aagagttaaa
    75121 agacgaattc tgatcaacga ttaagctaat ctgccacatg ataagagcga atatttcacg
    75181 gacttataaa aaaatttgaa aaatctttta cactaaatgt aaatatggtg atagatcaaa
    75241 aaaatgaatg taatctagcg atattgtata tagcaatttg gtaggacgtg cttgctgagc
    75301 aaattatttc ttggcgatcg catgggaacg aaatatctca gttgtgctga atgttatttt
    75361 gttttactga acgaaaagta accacaggta ccggcgaatt attgaccact tcggaggtaa
    75421 cgctgcccat caatacgcgc ttcaatcctg tacgtccgtg tgtacccatc gcaataacat
    75481 caatttcatt tttgacggca cattcgatga tgccggcgcc ttcggtatgc gctttgacca
    75541 tcatcgtacg gtaaatcaca cccgtaatgt caatccgaga aagaaaccgc tcaaaatcat
    75601 cctgaatgcc tttttcggtt tcgtttttat gcgaaacaga aacataaacg agcgtcaatt
    75661 cgcatgcaaa atcccgtgca taggcaataa tctgtttcat tgtttgacgg cacatatctg
    75721 aaaaatcaac agggatgagc attctgcaaa tatggttagc atcgcgtttc tggcgaatgg
    75781 tcatcacggg acaaggcgct tgacgcacta tgcgttcagc attagagccg atgattgcat
    75841 tgataccgct ttcaccatgc gtgcccatga tgatcatgtc cgctttgata ctacgtgccg
    75901 tttcgacgat ctgataatac ggcctgccga tcaccacgtg tttatgtccc acgaccccgt
    75961 gatgcagacg ttgaatcgta tcgtctaatt ttttatccgc ataacttgtt acattatccg
    76021 atagctggtg caggtcatta tccccgtaag gtcccatggg attgagcaaa tacgtcgctt
    76081 catccacata caaaaactcc agcgaagctt tatatttttt tgcggtcagt acggcatatt
    76141 ccaacgccgc atcggcgcaa tccgaaaaat cggtcgggaa taatatttta tctattctca
    76201 tatcataact cctgccatcc tacttaaaaa taggacaact cttaaaaaca caaaacgacg
    76261 cgtatcatgc taaacgatga taatgatcac tcaatttttg tgcccatttt ggcctgacgt
    76321 tctgctgctt ttttatccca ttccggcaaa atattcttta taaactcatc tttcgctgtt
    76381 ttaagttttg ccatgtccaa gccgataaac gcctgtgctt tttctttggt tgtgatatca
    76441 ggcatcgtga tcggccatgc cacgcctttg gccatcaaaa cctgcgataa tataatccgt
    76501 gcttcctgag ccttttggat gcctgcgcct aaaacgcgcg ccgcctcaag cggtgcatga
    76561 aatgacccgc cgtgactcgc ggcgacatag tcccagcgcc actgcgcctg gcgtatcaat
    76621 tgtaacgccg gcttcatttc atgctcagtt gcgccttttt cccatgcggt tttggcttcg
    76681 atatgtgctt tggctaaaac ttcttccaag cgtgaacgca cttcgatcac tttgtcttga
    76741 cgatcattca cattttttcg aagcgtctct tcactctcgc gatggcacac ctgacacgaa
    76801 ccggcgatat tgttaagcgg gctttggatt ttatggtcgg taaacttcac gccgccctga
    76861 ctacggtacg gcatatggca atccgcacag gatacaccac gttccgcgtg aatacctgtt
    76921 ttgaaaattt cgtaatccgg atgctgcgct ttgagcatcg gcgctttact tacggcatgt
    76981 acccaatcta catgttgcac cgagtcataa taagactcca tctgttctgc cgtaaatccc
    77041 ttgtcccaag ggaaagtcag atacttgtcc tcttttcctt taaaataata ttcgacatgg
    77101 cactgcgcgc aaaccaatga acgcatatcc tgatgatttg atttttccac ttccataccg
    77161 cgccgtttaa aagcttcggc taatgcaggg cgcgtgatgc ggagattcat cgtctttgga
    77221 tcatggcaat cctgacaacc gatgggatta acgatttcat ggccaagatt atcccatttt
    77281 tctttataaa aattgggagc gccgcgttca ttcattacgc gcggtacatc cgtacttttg
    77341 catgtccagc acgtaccggg ttgcggagcg ccggtgcgca aggttttccg aatatcttcg
    77401 accgcataat aatgtccgcg ggcttgttta aaatctttag aaaacgcata accggcaaaa
    77461 agaataacta cattcggata ttcagcaagt atatcgcggg tcgcacttcc accgtgttta
    77521 cttcgaaacg atgtatcttt cgtactcata tatgtttcat attcgcgcgg aaaattttca
    77581 ccccacactt cattgcgcgg ttcccagtca gcgataggtt tgaccatttg aaataatgtt
    77641 ttttcttcac ctcgacgctc catcacggta ttggcaaaca taccgacgag aaacacgaca
    77701 cctacggttg cacaaaaaag cagccagccg acccacggtt tttcttgaac cgtttggata
    77761 atgtttttca tacagtaaac ctcgatttaa gggtgaagcg tagtatctaa atttgttttt
    77821 tgcggatttt ttaaaaacga ttccatccaa tccggcataa ccttagccag acgaggaacg
    77881 cgtgcatagg gcgtggaggc caagctattg acgcggccat gtggcgtttc gcggtggcat
    77941 tgccagcaaa gaaggccttc accgtgccgt gattcggcat acgacgtacg ccccgctgac
    78001 acacgttcag ttagattttt gtgacaacgt tggcagtttt cctgcacaac cgccgcaccg
    78061 gcttctttga tctgaattac ctgcggttcc atgcgtaacg taaacattgt ggcatgacga
    78121 agcccgtctt gcgctttaaa aaaatacttt cgaattatat tatcgtgcgg aacatgacag
    78181 tcgttgcaca ccgcaacgcg tgcatggctt ccgtgttgcc aggttgcaaa ttgcggtgcc
    78241 ataacatggc aattgacgca cgtagccgga tcatccgata gatatgaaac agcattggaa
    78301 attttgagaa tcaccaatcc caagccaaaa agaataccca tgagaattaa aacagggaac
    78361 cgccattgat caggcggtat gaccagcgca ataagttctc gcagttttct catgatgctc
    78421 tgattccaaa aactttttac ataaaaaaga taacactatc tcaaatttaa cagtacatct
    78481 tactgaaatc acgcagagtg cactatgaca aaagtcataa aaatattgat ttactagact
    78541 gactaagtca cagacaaata tcaaaaaaaa tcgatgtttt ttttatagct gacaattatc
    78601 acttacaaac gcccatactg tcagatgatt tgtatatgct taaaaaacga ataacatcac
    78661 aaaaatagaa tcaatattgg ttttttttga tttttcgaac tgtgaaagcg ttgttggcga
    78721 cgatgcattg cgttaacttg ccagataatg acgcattaga acgttgtagt ggggagggca
    78781 tacgcaaaac gttcttcttg tatactagaa agctcgctga gccatcagga taaaaagaaa
    78841 aaagtatcaa tcttaaatct tattcacaac gcctatgtgt gaagcgattg ttttgcaaaa
    78901 attcattgcg gtcgtgtcaa attcataccc ctcatgcacg ttgcccattt gactcactcg
    78961 gatgtatcca ttcgttgacc ggtattgtcg cttcagaaag tcacgtaacg cttgcgttct
    79021 ctcacggcgc cggttggcgt agccattggt cgaaccatgt atgcacggtc tgataccata
    79081 atcgcccatt ctgcggtttg agtatccaat gcccctcgtc ggggtaatac aaaaatttgg
    79141 aagggatgcc tttgcgttgc aatacttgaa aaagatttac accttcgata accggcacac
    79201 gataatccaa ctcaccatgc acgacgagcg tcggtgtttt aaaattttgc gcatagttat
    79261 atggtgacca tttttcaaac gtctcccgat tagcaccttc ccagtaaggg ccgcccattt
    79321 cccattcgtc aaaccacatt tcttcggtac cgccatacat attaaattta ttcataatac
    79381 ttgcatggct tacaaaacat ttgaaacgat ccgtgttccc gttcatccaa ttcatcatat
    79441 aaccgccgta acttccgccc gccgcaccga tacgatcttt atcaatgaaa ctataggtcg
    79501 aacagatata atcaatacct ttcatcagat cttcgtacgg tttgccgccc cagtccattc
    79561 gtatttcatc ggtaaactgt tgtccgtagc ccgttgatcc gcgaggattg accatcacaa
    79621 cgacataacc gggtgcagca aaaagcatcg gattccatcg ccaactccat gtgtcgttcc
    79681 aggcgttttg cggtccgcca tgcaccaaat acactaccgg atatttttga gccgggtcaa
    79741 acttcggcgg tttcactaaa aaaccgtgta cggttgtatt atttgctccg gcaaaccaaa
    79801 attcttcagg agcggccatt tccaatgcgg ctaacttcgc tgtattcaca ttactgagcg
    79861 gcatactttt ttttccgtcc tcacgcacgg cgaaaatttc acccggcata cgcatactgc
    79921 tccggcgaac aatgatcgct ccttgcgcta catccgacaa aaggtcaaat gtttttgcgg
    79981 tgattttttt aggttcaccg ccgttagcag acatttgaaa aaccggatgg aaaccctgat
    80041 cctccgcatc aaaataaata gatttgccat cgcgcgacca caccagcgtt ccgacggaac
    80101 gatcaaattt ttccgtcaga tttttcgttt ttccggtggc tacttcatac agtttgagat
    80161 ccgtttgatc cgcttcatat cctgcacgtt ccatcgaaca ataggcgata tacttaccgt
    80221 ccggcgaata aacaccgtta ttgtctacgc cgggacttgt ggaaattttt ttaggctcac
    80281 cgccaattac cggcatcgtc catacatcgt tattggtgga ccaggctatt ctaggatccg
    80341 tattcatcgt aaaaatgatc gtcttaccgt ccggcgagaa accaaaatca tgagcaccgc
    80401 ccagatcaat cggaggcaca tcatagtccc ctgcgatcat atcggatatt ttgcctgtcg
    80461 ccacctccaa aagaagcagt tgactacgtt taccgtcttt aaacgaatcc caaacccgga
    80521 atggcaattt gccaatgagc tgcgctttga ctttgctgtt ttcggccgca tcgttatgag
    80581 cgcgattaca atcatccgtt tcacaatccg gaaacacatc cgacgtaatg agaatgtatt
    80641 ggccgttagg cgaccatacc aatccgttac ctgaaacctg cgtggaaaga ttcgtcacac
    80701 gttttggttc gccaccgtcc aatgccatag tccatatttg aaaaccacca agccgattgg
    80761 ataaaaaagc tatagtttta ccatcgggtg accagatcgg cgtaatatct ttggtcggtg
    80821 aaaaagtcaa ctggcgcgaa gttttgccat ctatcgatgt cagaaaaata ttggaagtat
    80881 acgtattgga ttccagcgag tatttacgca cgttgtacaa gacccaatta ccatccggtg
    80941 aaacagccgg ttcgtccaca cgccgcaaat caacaaaagc ttcgtatgtc agaatttttt
    81001 tatcctgcgc gattaaaacc gcagaaaaca atgttatcac taatataatt tgacgtagca
    81061 taatctatcc tcatgtaata aatgatatac taagtagtac ttaaaaaata attttagttt
    81121 aggcaagatg caagtagcag ctttacaaac gagatgatta tgacctgaga ataagcaaaa
    81181 aaaaagcgcc acagaaccca tctaactgtg gcgcttgcac gttatcccga aaccctaaaa
    81241 tcagaataac gaaccctaaa cccaaaaaag gagaaaaatt tcaaaaaact gaagttaaaa
    81301 ttcaaaaaac tgaattattt taactctatt ttattcttaa gtttataatt tacaaatttc
    81361 gaagaacatt ctcatatgct tacacatatt atataaacaa tacttatgcc aaaaacatct
    81421 ttgagtaaaa tcttgatgat tttatcaaaa acctcgcaat aaaaagctga ggtcggcgcc
    81481 cgtgttattg agttgaggcg tattaccttt ggtaaacaat tttgctccgt tttttccttt
    81541 gagggttaag gtgtcaccct cacgccggat gagtgcaccc tccctgagac caagcactgg
    81601 agcgttattg acttcatgaa attccgatat acgctggtca cgggtttcac ccatatgttg
    81661 ggtcgaagga tcagggtcta tgtaatgcgg attaatatta aatggtacca gattgagtgc
    81721 ttcaaaactc ggcggatata cgatcggcat atcgttggtc gtcttgatgg aaattgtagc
    81781 cacattggta ccggcacttg caccaagata caacatgccg tcgcttacac gatggcgaat
    81841 aatatcaatc aaatcttcgt catataattt ttttaaaagc cgaaatgtat tccctccgcc
    81901 gatgtgtatc gcttcggctt cggttaccat tttacgcgga tcgttttcgt gaatcccggc
    81961 cacacgtatt cccatctccg catacctatc cctcgttttt tttgtgtatg cattcagatc
    82021 atctaatgcg tatggtacaa atacaatttg ttttatcgcg cctaaaaaat caacgatctc
    82081 cgcagcccag tgatctagat aattttcacc aaagttagtc gaattactgc ataatagtaa
    82141 ccgcataaat aactccctat agttatggtc agacgaaatt aagcaaaaat tccgatttac
    82201 aaaagacatc cgtcatgaat agatggttac ctgtacttag aagagaacaa aaaatgaggg
    82261 gagttatgtg tcaatttata acgctatgtt taatcaattt attatttgtt ttttcatcac
    82321 ttcaagccgg cgatggtgat aaaaaaccgc tcagcgatgt attgaaaggt attgaaaaaa
    82381 aacataatgt tgttttctct tatgtaccgg ctttagtaca aaatgttcgc gtcaatacac
    82441 cttccgaatc cgataaactc aatcaccaat tggattatgt ttttgctcaa acggatctca
    82501 cctaccgttt tatcggaaca aaagaaataa cgctatttaa aaaaacaaaa accaatagca
    82561 acattgaagc tacggcgcaa gcgcgtttgc aagccggaag atcacgcgcc gatgaagcgc
    82621 tgagtaccat gaataaaact ttggatggtg aacaagaatc cggcgaacac gaagacgatt
    82681 cattaccgac cgataccacc gtatccattg ataaaaatga gacctctcct tctatcgtga
    82741 taccaacacc catgtcgcca caaaccgaaa cgcaaagtgt acctactgaa aaaacggagc
    82801 cgcacgactt gaaaaaccga agcgttcgta gtgaattttt gggcatggat ttcgctccca
    82861 cgacacgcat cgaaccggct tacgtgcccg tgccgctgca tgcaatttcg atcaacatca
    82921 tttctactcg ccttgtaagc attgagggtg ttgaagtagg gcttatcgga aattacgacc
    82981 ggatgtatgt taacggatta caaatcgccg gaggaggaaa tcgcgcgtat cttcacgcac
    83041 gcggtctaca gatcgccgga ggttccaatc aaacaggtga tttgtccggc gttcaaatcg
    83101 cgaccgtcaa cattgcagac caaacgaacg gtcttcagat cggtgcagtc aatatatcca
    83161 aaaaagatca ttccggttta caaatcggcg cggggtacaa tcatgccgaa aatgtacgcg
    83221 gattgcaact tgcttcggtc aatgaagcca gtcatgtaca cggaattcag atcgcattgg
    83281 tcaatcgtgc aaaacaagtc catggcgcac aaataggact gtttaatttt gcagacgaga
    83341 acaacgggat cgcgctgggc ttactcaatt ttattcgcaa cgggcaacat cacggacgtt
    83401 tagcgcgcac cgagcgcggt ttcaaccatt tttcttatac tcacggcagc aaatatatgt
    83461 acacgatata cgatgtagct gttcgccaac ttcgtgaacc tgcacaatgg gctctgggcg
    83521 gcggtatagg cgcaatagtt tggcccgatc ggcgtttatc actcgctatc gaggctacga
    83581 cccaccaaca caataaagat aaattttggg aacaccggct caacatggta aacacattaa
    83641 aaatcacacc tacgattaaa ttacacaaac atctatccgt gtatgccgga gcatcagcca
    83701 atgtatgggt atcgcgcgct gaaaacggtt ttccggctcc gacgaatgcc tacaattggg
    83761 attataagca taccacgatt cgtgcttggt ggggcggatt ggtcggaatt tatttctagt
    83821 cgatgcctgt gcaatacaaa taatatagat tacgggtaag acaaaaaatt ctgtaacctt
    83881 ttgtttattg cctcccttcc catgttgcgc tatattatta ttctagtttc atgaaaacaa
    83941 tgaaaactct ataggggtat gaccaccgag gaaatccaac tcttaaccaa aaaaatacgc
    84001 aactctgatg ctctggcttt tcgtacgtta ttcgattttt tccatcgtcc gttgtatcat
    84061 tttgtgtttt ataaaaccta cgatagggca ctatccgaag atattgtgca ggatgttttt
    84121 attcgattgt gggaaaatcg attcaatctt cgagaagatt tatccatcaa atcctatctc
    84181 tacacgatag ccgttaatct tactctcaac actatacgtg gtcatacaat tcgtaccggt
    84241 gagggaatcg aagatcctga tacccaaccg cacgaagaaa cgcctctgtc tgttttggag
    84301 aaaaaagaat ttcaacaact acttaattct gcgattcaaa aattacccga acaagcacgt
    84361 atcgttttca tgatgagtcg ctatgatgac ttatcatatt cagaaatctc cgaacgcctt
    84421 tccattagtg ttaaaaccgt cgaagcacac ataggtagag ctttgaagca attgcggact
    84481 acattaaaac actgacccat ttcccgacac ttatcctcat taaaaacaaa tctttaattt
    84541 ttatttattt ttttacttat gactaagggt ttttcctttt taaagtgtat agtctttgga
    84601 ggttatttaa tttactaatc aggaggatac caagaagaga gattgtcttt cgtgcatata
    84661 tatatccact ttctttgcac tggctagctg taaagataaa gaaacaaaag aacacgatgc
    84721 cgcatttttt tgggaacatg aatttggcaa gaatcccgat acggcgaaga cagcatagat
    84781 agtgtaccta cagaaatcga acgccttggt ctatccggag gttcatccat aattacgttt
    84841 taggcaaacg gtactttttt taatcagcgt tacgaagacg gtgaagacat gatgcattgg
    84901 tactccgacg gaggccgatt ttatgctgac gatcgtggcg aagtcaattc attggaatat
    84961 tctattgcgg agattcacta aagcaaatcg aatccgacga tgccttctgg agtatagaaa
    85021 tctatatcaa acaaaataaa taaccatgag tgaaaactac tatcaccaag atgactttat
    85081 cggccgatgg atcgcaggta cattgaccga cgaagaaaaa aaagaatttg agctgtggct
    85141 aaaaagcaac tcccatcagc aagagttttt caatgaatta acgaaaattc attggtcaca
    85201 aaaatcctta gagatcccaa aggctccaac cgagcgcatg tggagcgcta tcgctgaagc
    85261 aactacatcc aaacaggttt cacgaagttg gatcagaaac acaacgcaat gggctgcagc
    85321 catagccgca agcattgtca tttactttgg gttggagtct caattcgctt acagagaaat
    85381 aacaacttca aaggcacaac aaaccgaaat cgtattatcc gacggctcta tagcaagact
    85441 caatgggcta tcttcaattc gctataactc ctggcgatgg ccgttaacgc gcagcgttac
    85501 tatgcatggc gaagtgtatt tcgaagtgca aaaaatgaaa cggcctttta atgtaactgc
    85561 agaaaatttg aatgtggagg ttttaggaac caaatttaat gtaagggatc gtgcagaccg
    85621 ttcagaggtt gtctgttcat ccggaaaggt acgcgtaagc gcaaggaaca actcagaaaa
    85681 tacaattttg acaccgggtc tcgccgtaag tatgcatcag ggacattttt ccatgccgta
    85741 ttctatggat acgacgctag ttgcaggatg gtcgcaagga atatggaact atcaatcagc
    85801 tccgatttat tcaatcttaa acgattggaa aatcacctat ggactaacca ttactatgga
    85861 tagcatagat accaaacgca ctttcaccgg attgatccac ggttcatcca gtcttagcgc
    85921 tttaaaacaa ttaggattgt ccgccggttt tgaaaccaca atcgtcaatg attctacttt
    85981 tttcttaaaa ctaaaaaaat gaattttatt acttcatgaa cttctatcgg gtcttttttg
    86041 tagctttttt tttcacgact tatctaactt ttgctcagga gaaggagatt gcttcttcct
    86101 ctgctcaaag tgccatatct ctcaaacaag ccgtcgcaga attggagtca cggtaccatg
    86161 tgcgattttc atatgttgac cagaccatag caaaatatac tgtccgtact ccgatcaaag
    86221 gcgatgattt gcaacaatcc ttagagtata tattgatcgg taaaccactc gcatacaaac
    86281 gcatcggaaa aaatattata gttttgtatg aagacgacac caaaaagcaa gaagcttcag
    86341 gtcagttcac atctgtacgc ctaacgatcg ttgatgcacg tacgtcagag gggatttatt
    86401 ttgccaacgt aacaatctcc ggtagtcgtc gcggggcatt gagcgatgca catggccaag
    86461 ctgtagttca gcacattcct tacggggatt acacactacg tataacagca ttaggttatt
    86521 tgcctttgga caaaacgttg catctttcat ctcaagaaat cactctggat acgatacgtc
    86581 tcgaacccag cgacattcga ttggactcca ctatcgtctt tggtgaaagg gaaacctggc
    86641 gtgtcagtga tgccaaattg cgcgttcaac ccagcatcat cgttatagat cgccgaaaaa
    86701 tcaactcaac tccgagtgtt cttgaacctg atctttttag aaccttgcaa acactacccg
    86761 gcgtcaccgc tcccaacgat ctatccaacg aactgtatgt acgcggcgga actccggatc
    86821 aaaatctaat cacaatggat cgtgctatcg tatatcaacc gtatcacctt ttcggtatag
    86881 ccggcatatt taatactgac gcaatcgaac aagtgaattt ttccaccggc ggtttttcag
    86941 cgcaatatgg caatcgtctg agctctgtaa ttgatgtacg cactaaatcg gcttcaccaa
    87001 ataagttctc ggccacgggt aacgccagct tgctcagttc caaaatcacg atggatggtc
    87061 aacccagcaa acattggtat tacctcattt cattgcgtcg gacctattta gattttgcca
    87121 ccaaagcggc ggtatggtta aaaatagctc cagaatcaat tccttattct ttttatgacg
    87181 gactaagtaa agtagtgtac cgtccgaata cactgaacga atttggtact tcattgtttt
    87241 tcagtcggga taaatttttt tcaaagcaca gggattatga atatgtcttc aacgaattta
    87301 acattagtcg aaagttacct atgtatagcg tcggccgcct tgactatggc tggggaaatt
    87361 taaatgctgc cgtatattgg gactggcagg caggagatat tttccgatcg cgcgttacga
    87421 tcagccaatc caaagctacg atcaaactgg atgaagacct ctattacggg tatgacagcg
    87481 gtgcgccaga ttcgatccgt cggcggacag atagccttaa tgcattacag gaaaagcgtc
    87541 cttttgatac gcgaaactat atcgtagatc gagggttcgc atgggataca caatgggaat
    87601 ggttaaatga acacagtatg tatctgggcg tttcacttaa cttgatcgaa atgtcctatt
    87661 actggaaaaa ctttaacgca ccaccgcatt atgtacaagt gttttacgat tttgcgccgg
    87721 acagctttcg ttatcagcgt cgcacaacta atctcgcatt ttatatcgaa gacatgtgga
    87781 aaaaaggtga ctggacgatc aaaccgggta ttcgaataga acatttttca agcaacaaac
    87841 gatctctatt gataagcccg cgtacggcga ttcggtatga tgtctcgccc aaactagcgc
    87901 tcaaaggaag cgccggcata tattatcagt ctctttttac tgcgcgcgag cgcggctata
    87961 tcggtttttt agaagtgcct ttctccactg aagatcaggt tgaaaaagcg actcacatca
    88021 ttgcaggtgg agaatttttt ccaaatccaa acattagatt gacggcggat atctatttca
    88081 aacattttaa tcgcctttac cgaaaggacc actcatcaga tagccttcac tttgataaag
    88141 gaggcggtga cgcatacgga ttagaaatca cttaccgtcg aataggtaat aaaatgagtt
    88201 ttgaggccga ttattcttta gcctgggtta cacgcacatt tgaccaaaat acatatgtca
    88261 ctaattatga ccagcgtcat acattaaatt tactcctcag ttatcgcttg ccaaaaaact
    88321 ggactgtgga tgcacgatgg attctggctt caggtcgcgc ctaccgacct acggagtttt
    88381 atggcgcaac agcatatatt gaacccggca gcggcaggtc gcttatggaa gataatggtt
    88441 ataatatcag tcaagaaaat ttggatcact tagagtttta tgatcgttac ccgctgtacc
    88501 atcggcttga tgtgagcttg gtcaaaaccg ttcgtttcaa aacctggaca atgaaacctt
    88561 atatttccat aataaatacc tactattcta ataatccttt gttttacgat tacaactaca
    88621 atggtagtat aatctcaaat gaacctgatc cccaacgtat gaactacagt aaacgaaaag
    88681 gatacggcgt tcctatttta ccaagtttcg gaactttttt tgagttttaa aggtgaaaaa
    88741 aaaatcaatg acatttgtga tggtggcgct tttcgtaggc agcgcctgta accaaaataa
    88801 taataatgag tttttaggcg ttttcggaat tttgatgccg tatgcatccg aaaatgcgac
    88861 gatcattctc atgcgtactt acagccagga aaactacgat gagtttatac ccgaagcgct
    88921 tcagataaaa gaatctaacg ctgaagttat tattagcagc gattcgcaac acgtcacgct
    88981 ttcagaagta atacccggcc gctatgtgga tacagccgga atgttaaaat attatccggg
    89041 gttgatttat aaacttgagg taaaagccaa ggacggatta agcgttcatg cgcagtgtgt
    89101 acttccgggc gtgccgattc ctgaccctga gacgacgccg gatagtttta gcgtagcgat
    89161 agagttggat acggttcgcc gaatagtttc cggatatccg tatccggttt tgacacagat
    89221 gtattatctt cgttctccga cagtatcgtt tgactggagt gatgcaaccg gagctgaagc
    89281 ctattattgg gagattggcg ctgacacttc ttatcttaaa gaatccgatc gcgaattttt
    89341 cataccaaac tcaaaattat ttcagaccga aaaatggatc gcgtattcat cgctagtatt
    89401 atttgatcac tgggattcta caacttatcg gggcctttat aataagccgg ataacccttg
    89461 gacatggagt ggtttttata tcgattcgga aagggagttc gtttatacat cacaactcca
    89521 tattaccgcc atagaccgaa atttcattga ctatacgtat tataaccaca aatctaacat
    89581 ccgcggcgga tacgggtatt ttagcgccgc cacaaggcat aaacgaccga taaccgtcca
    89641 tgtccaatcc aaatggaagc aacgatgaaa gttttaaatt acaatacaat tctgatgtgt
    89701 ctgtgtgttt gtttgacggc atgcagcgaa ggtatggata catcttccgg cgataaacaa
    89761 ccaccgcact ttgaatggga cccaggttct gcgactgtaa ttctaaagga tatgattcaa
    89821 tacggagaga ataacattgc cattctcagt ttacactatg acacaaatcg ctatggtttg
    89881 acggggttta cacccaacgg aatccagaag tttcaaaaat ttttaccggc tacacactat
    89941 attgaatcgt catattttga aagaaattta agttatagta acggcatatt tacacttgta
    90001 ataaacccca aggacacagg ccaaaattcc ctttttattc aacttgatac taacggaaac
    90061 ataaccaatc aaaaagagct tgtgcaaata aagtacgctt ccatatttcc gatgaataac
    90121 ggcgaatact tagtgcgatc atctattttt gactcattac aatttaaaac aaaactaagt
    90181 aaaattgata acgatggcga acttaagtgg gaaaaagtga tgccggtttc tttcgaatat
    90241 ttgcatgtcg ccaataatag gatctatgga aagtggaata atgacagcat tgtatcaata
    90301 gatgcaaacg gcgacgtact ttggaaacaa agtcttatgt tgccaaaggg attcttgtta
    90361 tgcagggttt cttcgagtga ctatcgcatc gccagaattc tatataatcc atacaattat
    90421 atccctgatc gcctagccat tgacgcgatg gatattgacg gcgctataac cccgctggat
    90481 actattccgt taaactggag tacagatttt accgattttg aaagcctcgg agcgaatgaa
    90541 ttggttttat taggtcgatg gggtggtagc aataaaattt tgctcatgcg gcttcaaagc
    90601 gacggctcct gggtagaatc acatttagca cccgatgaaa ataatttttc tcttgtctct
    90661 tttcacgcaa acggcgatgg tacttatttg attatggggg ctatgtatat aaattcttcc
    90721 tattattcga aaacgttgat atataaaatt gatgcaaacg gaaactattt gccgttttaa
    90781 aacttttcat gaattgcctt cacattaaat tattctacgc attcgcattg taaccgaccc
    90841 attaggaagc atctgatcga aaactttaca ttcgattgat accgccttga atttcttctt
    90901 tcactctcgt attattgccc gccgaaaata atcattttaa aactatcagg cgtattttcc
    90961 atttcttaat caggagtttt gatcgcatgt ccgaacagga aattctgcgt ctcgttgcca
    91021 aagaactcaa cattcccgtc caacacgtca cttcaaccat tcagttgctt gacctggaaa
    91081 acaccgttcc ttttatcgca cgctaccgaa aagaagtaac cggcggcatg gacgaaaatc
    91141 aaatccgcga tatacaggat aagatgacat actatcgcgc attggaagca cgtaaagaag
    91201 tcgtactcaa gtctatcgaa gagcaaggta aacttaccga cgaactcaaa gcgcgtatcg
    91261 tagccgctac aatgcttcaa gaagtcgaag atttgtatct gccgtataaa ccaaaacgtc
    91321 gtaccaaagg tattatcgcc aaagaaaaag gcctcgaacc gctcgcttta caaatcctcg
    91381 cacaggagat tacaagcggc accctagacg aacatgccca acccttcatc aatctcgaaa
    91441 aaggggttac aaccgtcgac gaagcgttgc aaggcgcacg cgatatcatc gccgagatca
    91501 tcagcgaaga tgcggatgta cgaaaagatg tgcgcgaatt tacacgtgag aacggcatcc
    91561 tcgtgtctga agtgaaagaa gaccccgaaa acgaatatga aatgtatcat acattcagcg
    91621 agccgatcgc caaaatgcct ccgcatcgta tcttggctac caaccgcggt gagcgcgaag
    91681 aaaaactccg catcaaaatc acggttgacg aagaagcttg ttgtaacatc atcggatatc
    91741 gccacgttaa aaaacaaaaa tccatttttc gcgatcaggt tatcgaagcc atcaaagaaa
    91801 gttatttccg cttgatcgcg ccttccattg aacgtgaaat ccgcggcgag tttaccgaaa
    91861 agtccgaaga acatgcgatc gaagtattcg gaagtaatgc gcgaaaccta cttctccaag
    91921 cccccaccaa aggcaaaatc atcatgggca ttgatcccgg atttaggacg ggatgcaaag
    91981 tcgctgtcat tgatgaaacc ggaaaatacc ttgagggcga aaccatctat ccgcatgaac
    92041 cgcaaaaaga cgctttgggc tcaaagcgcg ttttgaaaca attcatcaga aaatatgaag
    92101 tgagcgtcat tgcaatcggg aacggcaccg cgtcccgcga aacggaatct ctcgtcgccg
    92161 atctgatcgc cgatctcaaa aaagaagatt ctcatctgga acttgtgtat acgatcgtat
    92221 cggaagccgg ggcttcggtt tattcggctt caaaaatcgc acaacaagaa tttcctgatc
    92281 ttgaagcctc acaacggggc aatatttcca tcgcacggcg acttctggat cccttggccg
    92341 aattggtcaa aattgatcct aaatccatcg gcgtcggcca gtatcaacat gatgtcaatc
    92401 aaaaacgttt ggccgaacag cttacacatg tcgtggaatc cgctgtaaac tatgtcggcg
    92461 ttaatcttaa cacagcctcc gctgctcttc tgacgtacgt atccggcctc tcatccaaaa
    92521 cagcccaatc catcatcgca catcgtgata aaaatggaaa attctcgcgc cgtgaacagc
    92581 ttatggatgt gaccggtatc ggtccggccg catatcagca atgcgcaggg tttttacgta
    92641 ttccggacgg cgataatccg ctggataata catccattca cccggaatcg tatgacgcca
    92701 cacaaaagct gctcaaaaaa tttaatatcg tcgatccgtc aacagccgga agcctgcttg
    92761 agctgcaact gcgtaatgca aaaatgtctt ttgaaaatat agccggggaa gtcggtgtgg
    92821 gtgttcctac actcaaagac attttagata atcttaaaaa accgggtcgc gatccgcgcg
    92881 aagaaatgcc gaaacccatt ttcaaatccg atgtattaaa aatggaagac ctgcgcgaag
    92941 gtatgatgct caaaggtaca gttcgcaatg tcgttgattt tggcgctttc gtggatatcg
    93001 gggttaagca ggacggtttg gtacatatca gccatttatc caataaatat gttaaaaacc
    93061 cgatggaggt ggtggctgtc ggcgatgtgg tggatgtaaa agtaatgagc gttgatctca
    93121 aaaagggacg cgttcaactc agcatgaaag aagcctcgcg aagctactag aacaaattgt
    93181 attttcgaga caaaaaaagc tgcttcattt gaagcagctt ttttattgct aagattgtaa
    93241 atttcagaat tttcctgtcg caatttctgc tactaaatct ttaccgcgac ctgcgatacc
    93301 gactttatca tcgccataca taccgtcatc tttgatgtcg cgtgcgtaag ccgcttcgcg
    93361 tttggcaata agtttttgaa ttttcatcac accttcgatc aagccttcgg gacgtggtgg
    93421 gcaccccggt acatacacgt ccaccgggat aaactgatca acaccttgaa tgagcgtgta
    93481 ggtgttaaaa aaacctccgg tcgaagcgca agcgcccatc gaaatacacc atttgggttc
    93541 aggcatttga tcccaaattc gtttgagcac aggcatcatc ttgatcgaga cgcggccgga
    93601 aacaatcatc aaatcagcct gacgcggtga aaaacggata gcttccgacc cgaatcgcga
    93661 catatcatag cgcggtccga gcgtcgccat catttcgatc gcacagcatg ccgtaccgaa
    93721 aggcataggc caaagtgaat tgcggcggcc ccagtcataa acggcttcga cggtcgtcag
    93781 aagcacatta tctttgagtt ctttcgtgag atccataaag gatgtttcct tttttcgaaa
    93841 atctaaaact tatttggcgt tcgcgaagtt ttcggcaact ttgttccagt taaccacatt
    93901 ccaaaacgcg gtgatataat ccggacgacg gttttgatat ttaagatagt atgcatgttc
    93961 ccatacgtct aatcctaata taggcgcacc tttaacttcg gcaatatcca tcaacggatt
    94021 atcttgattg ggggtagagc atatcgccaa tttgccttct tttacgatga gccatgccca
    94081 accggaacca aagcgtgtcg caccggcatt tgcaaattga gttttgaaat cggcaaaaga
    94141 accgaatgcc gattggatcg catccgctaa tgcgcccgtc ggttcaccgc cggcattggg
    94201 gccaagcacc gtccaaaaga gcgagtgatt aaaatgcccg ccgccattgt tgcgcacggc
    94261 gacaggatat ttggaaatat ttttgcagat ttcttcgata ctcaggtttt cttcaggctt
    94321 accggccacc gcgttattga gattggtcac ataggcattg tggtgtttgc catggtggat
    94381 ttccatagtc atcttatcaa aatgaggttc caacgcatca tgtgcgtacg gaagcgaggg
    94441 aagtgtaaaa gccattgtat attctccttg agcttgtttt tttactggtc aatactaatc
    94501 aaaatcacct tattttgcaa tgtttttcca tgatcatagg cccatgattt tgatcacaac
    94561 gcgttttttt cgttgcccgt caaactcggc ataatagatc tgctgccaag gaccgaaatc
    94621 cagccgacca ccggtcaccg gcacaatcac ctcatgatgc aatagcaagt tctttaaatg
    94681 cgcatccgca ttgtcttcgc cggtacgatg atgaaggtaa ttcggattag cgggcgccag
    94741 atgatccagc catttatcaa tgtccttgat aagaccatct tctgcatcat tgacatatac
    94801 cgccgctgta atatgcatcg ccgacacaag cgccatgcct tctttgatcc ccgaaacgtt
    94861 aagcgccgac tctacttcat cggtgatatt gatatattct cttctttgtt tagtgttaaa
    94921 ccaaaggtag tgtgtatgaa ctctcacaag tcctcctttt tttaaaaatc tcgttattcc
    94981 atttcaattt cgcaagaatc gtgtggtttt cagaaattga atagcacata ttatcgcatg
    95041 ttaaaatcct acatacaact tccgcccgac ttttcgtttg acgaatgcct tgtttttctg
    95101 aatcggtctg atcgggaaat acttcatcgc gttcacaaca aatccctaac taaagctttt
    95161 cgtataggcc acgaaacttg cttaacttca ataaccgtta aatcggataa aatgatgctc
    95221 cgttttgaaa gagacatctc caacgaagcg gcgcgtttta ttcgttcaga agtttccgaa
    95281 tggtttgacc tgtcatgcga tctggcgaac gtttatcgtc ttatgaaaaa agatcccaaa
    95341 ttattcgcac ttaccaaacg ttttcacggt ttacgtttga tcggtattcc cgatcttttt
    95401 gaggctttag cttgggctgt cattggccaa caaatcaatc tggcttttgc ctatacgtta
    95461 aaacaacgtt tgattgatgt tgccgggcaa tcgcttactc atgacggatt accctatggt
    95521 ttatttccag atccggccac cgtggctgat ttgagggtag atacgttacg aaagttacaa
    95581 ttttcaacgc gtaaagcaga atatcttatt catatcgcgc ggcaaatcgc cgacaaaaga
    95641 atcgagaaaa cggctttgca aaattactcg ctttcgcaaa tagttgagcg gcttacggcc
    95701 gaacgcggca tcggcgcgtg gacagcgcat tatgtggcga tgaagtgtct tcgtcaagcc
    95761 aatgcttttc ctatcgggga tgccggatta catcaggccg tacttcatat ttatcaaatg
    95821 aaaaagaaac ccacactcat acagattgag aaaaaatcaa aattgtggca aggatgcgaa
    95881 gcgtatgctg tcttttattt gtggcgaagt ctgcaggaaa catgaagtga tttaatgaat
    95941 catctgaccc aaacgatccc aacgaatatc aaaattatcc tgacttgccg tggaccagta
    96001 aatcattacg gcttgtccga taagatgatc tttgggaaca aatccccagt ggcggctgtc
    96061 ataactccga tcgcggttgt cccctaaaac aaaatatgaa tcttccggca cagtgaccgg
    96121 cccatagttg tcacggctac cggcatcgcg cggaaaaaca gattcgtatc ctttattgcg
    96181 aggtataatt tccggatcct caaattttaa acccggcggc cggataattt ccgaaaaatc
    96241 tttgccgtcc acatacacga ttttgtcttt gatctctact gtttgaccgg gtaatgccac
    96301 aagccgcttg atatagtcca ctgtcggatc aggcggaaac ttaaaaacaa tcacatcccc
    96361 ttgtttgggt tcacgcacag ccggaaactg atactgcgcc aaatctatgt cggtaaaaat
    96421 caaaggtatt gtcgcaggcg tctttacacc ataaatacac ttattgacta aaatgagatc
    96481 accgacgaga agggtatcct ccatactgcc ggtcggtatt ttaaaagctt gcacaaagaa
    96541 gagacgaacg ataatcgcga taaaaaccga aacgaatgca atctggaaaa gctcggttgt
    96601 tttattatga ttgtctgaca atggtttttc cacagcgtta gattctcccg ctgaagatac
    96661 gtcggatgac gatgtcatgt ccatatgtcc ggccggttct tgcatttttt tagcgaataa
    96721 tcgttccgat acgatgccat ttgacagaga aaaaattgaa catcataagt actttcatcg
    96781 gaatggacga ttgtttagcg gcatccgtat tggaccaata aaccaacata ccttgacctg
    96841 caatgtggct acggggcacc ggcccccaat agcgactgtc caaactgtta tcgcgattgt
    96901 cacccatcat aaaaaaataa tcctgtccga ctgtataata cgttgactta acgccatcaa
    96961 cagtccattc cccgccgcgc atctcaagtt tttttccgtc ccgcaatgaa agataccgga
    97021 ccaattcata atttgtcaga ttcaattcaa tttggtcacc ttttttcgga acgcgaaacg
    97081 gaccaaaatt atctccgctc cccatgtggc gcggaaaaac ttgcccttcg gaacgctcgt
    97141 aaggatcaaa gtgaacaccc ggtaggtcaa attttacaga taatgcctca ccatcaacaa
    97201 acaaaacttt gtttcgtaca tacaacgttt gaccgggcgc cgctacacaa cgcttgatat
    97261 aatctaatcc gttctgactc ggcatcattt cagggtgggg gaaacgaaaa accacgacat
    97321 caccttgttg cggttcttta aacgcaggaa gttgaaactg aggtaatttg atatccgtga
    97381 agggaatatt ttccggcgat ttggcgccat aaataaattt atttgcaaaa ataaaatccc
    97441 cgggcaagag cgacacttcc atcgaacctg ttggaatagt gaagttttgt acaacaaagg
    97501 tgatcgtgat atagtaaacg catagcgccc aaccaaacga tttgacattt tccacaagtc
    97561 gctcggtaaa ggtcatttct tttaggggtt ttccgggcac cggattattt tttttaggtt
    97621 cttctttttt gccaaaaatg gacatgctac ttttgcactt tcagtttttc tttaattcgt
    97681 tgttctataa aagccgggac aaaactcgat aaatcgcctc cgtattgcgc tacctctttt
    97741 acaatagtcg aattcagata tgcatatttt tcgccgggca taagaaacat cgtaactatt
    97801 tgcgaattga ggcggcggtt cacgtgctcc atttgcagct catattcaaa atccgaaatc
    97861 acacgaaggc ctcgtataag cacgtgcacc tggcgactga ccgcgaagtc aacgatcaat
    97921 ccttccgatg gctcgcatcg cacattggcc atatcttttg ttgcctcggc gatcatcgct
    97981 acgcgttctt ccggtgaaaa caacggcttc ttttttgaat tattggcaac cgcgacaata
    98041 atctcgtcaa acaatgttgc tgctcttgcg atcacatcaa gatgccccaa cgtgagagga
    98101 tcgaatgtcc ccggataaat cgcacgtctc atatttaatt cgctttattc attaaaatac
    98161 tcacggcact ttcgccccag gttttttgct tatgcatcgt taatccatgc gcaggtaacc
    98221 actcattttc aatttcttcc ggtgaggttt cgattactaa aacgcctcca ggatttaacc
    98281 attgtccggc atctattttt ttagctatca tagctccaat tcgcatcgca tacggcggat
    98341 cagcaaaaac aatatcatac ttttttacgg caacggacga tatgaactct aacgcatcgc
    98401 cggtgattac atcaatttgt tgctcaaacc ggcattttat tacattgttt cgtaacacgt
    98461 gtacggcctt ggaatgaagt tccacaaaag tagactttgc cgcgccgcga ctcagcgctt
    98521 cgattccgag attgccagtc cctgcaaaca aatccaaaat ctgcgtatcg gtaacatcta
    98581 tgatgtttgc caaaatatta aaaacagatt ctttaactcg gtctttggtc ggacgaataa
    98641 tctcttcatc gaatgcattc agaaccaaac cgcgtcggat accggctata attcgcatat
    98701 aaaaaataaa gccgaagggc cacctcggct attggatttt cattaaaacc gcattggtaa
    98761 ttattttgtc ttcgttgagt cggctgattc cgattggcga ggcagtagaa tactatactt
    98821 cataaaaata gtatccggca ccagtttctt cttatcggcc tttcggcggg ccgaataatc
    98881 agcatattgt atatcaatct caccgatttc gaagttgaaa cgcatttcat tcagctttgc
    98941 catgtatttg aagtaatccg aaaccgaccc ttcaagtttt aactcaccgc tgtgcgtact
    99001 catgactacc cctttggaag atgtcatttt accgtcttgt aatattttca atccggcatt
    99061 gcgggcaccg tcgaggattt tttgtttggc tttttccaaa tcaagataga ttatttttaa
    99121 cgcgactatc tccgaatcac tcggcgtcga aaccgttttg agtgtaccgg acatcgtacg
    99181 tttgaatccc aaacctttga ctaacgatgt cggtttgatc gcttgcggtt tggtttcgtc
    99241 tatgcttttt gattctaaaa acaaaacggt aaaatcttcg agatctttct gtgttttgcc
    99301 aaacacgtca aaagcatagt tactttcttc cacaccgagc gaagtgagat gccatttttg
    99361 cgaattcgca gacactatcg aatccgtcct aaataaaaag cggcggtacc gctcctgata
    99421 gcgaatcgtg ttgtacatat acagatttcg gattttttcc tggcgcgctt cttcggccgc
    99481 ttttgccgcc gtctccatat cttgctccgg caaaaggtgc aaaggccccg tatattgctc
    99541 taaccacgcc acaacggcgt aacgttccat ccacccgtat gtcaaaccgc caacaagcgc
    99601 gacaaaaatc aaaataataa acaaaagccg tcgttggctt tttttggcct cgatcgttat
    99661 cgttttgccg gccgacgtat cggaatccgt tttttcgtgc tgagcaggcg gcgcaaaatg
    99721 gttttcaagc agatttatct tgatcatatc aatccataag aagtttgatg gccgctccta
    99781 ccgcttccgc atacaccgca taatccggag gcgctatgac aacttgcgat ttattatctt
    99841 tttcgtcggc atccgcagat gcagattcgg agtcttcatt tttttcatgc ggccggaatt
    99901 cagccgggag ttttatttta cgaaaaggct caattaccga tggattaaaa tctgcttcta
    99961 tgtatggcaa tacttcttgc gcaaggtcat gacataatat caaacgtcct acgacggagt
   100021 tggtattacc cgccgttgca tgatatgcct gcatcatatt gggtatatgc gcattgataa
   100081 gctcgcctat tttttgcggg gtacttttgg gtgaaacggt aaactgatat acgtgctgca
   100141 catcgtaatc tttgcacaga agtaaagtca cagaaccggt cttgcattcg gccagcacgg
   100201 tcataccgtg atatgaaata tcgtaattca cttcataggt cgcatgcgtg cataatatat
   100261 ccacatccat gttactgaac tgaattttac ttttatcaag caccgaacgc aaactatcgg
   100321 cataacgacg acggataccc accatcagca taggatccat cggtgccggc gatgtcttct
   100381 caagcggaac cgtattcaaa atatattgat caaaatcgtc cgtaacttgc tgacggaatt
   100441 cccatttgat ataatcctga aaatctgact ccgaacgctc actctcaacc agaaccttat
   100501 gtaaggttac aagattggta tttaaactga tattcatccg tttggcgcga ataccattat
   100561 cctcaaggca gcctttgagg tacgcaaaaa gaccttgtac gttacgttca acaccttcaa
   100621 tgccggtacc aaacttcata gtcgtctgaa tggtgcccat ttttctgacc gaaaaaccat
   100681 cttccgaacg tagtatttcc gcaaaatgaa tctgttgtcc gtgaaaactc aggccaaacg
   100741 cgaggttacg atattcacct gctcgacgtg cataatcttg atctatggca tcatgatcca
   100801 gatcgccgcg atcgctttgt atactcattt gtgacataat actaacgcta tctcagtaag
   100861 taagttctag tttttcattc caagtaacac gactatctat gacggccccg attcgctgaa
   100921 tttctgaatc cacggacgat ggttttatat ggaccgtctt gcggccatct ttcaaaccgg
   100981 tttctattat aaatttttgc ccgttcatcg gattatcagt caaagattca aaatactgtt
   101041 cacggtacgt cgcgggcgta acatctttga agtactcact atactcggga aatcgcatca
   101101 aggcctcaat caccggagta aaaacttcgt ccggaacctt gtcttttcct tttttattaa
   101161 cgaaatcgaa taatttgccg ttatcggatg taaatgagga gtcggaaaca aaatatgctt
   101221 tttgtaccgc caacaaagcc ttcattttag cacgtgttaa tcgaatttta tttcgatccg
   101281 ctttctcggc ttgcacgggc gaaaccacac tccacactaa aaaaacaaac agcaaaacaa
   101341 taattgtttt gagaatataa tccgtgatgc gtgttttagg aatggatgcc ataactcagt
   101401 taatatctat gaatggccta attttttcaa tgagtttctt cccgacacct ttgacatttt
   101461 gaatatcttc caacttagaa aacggcccgt ttttattccg gtattcgata atacgtgccg
   101521 ctatcttagg accgacaccg ggaagtgaac ttatttcctg cacatccgct tgattgattc
   101581 gaaccactcg tgcgggcgcg tcgtttgttt ttaatataaa ccgttgtatc gaatctcgtg
   101641 caacaccctt gcgaaacaac gtttgcaacg tatcttggcg tgaaaaccat actgaaaagg
   101701 cactatcatt ttcagacttc tgaggataag gacgggcctc gttcttggga taaggaacgg
   101761 atgtaatctc tatcgtgtca tcacgatacg tttcttttat cagacctact aatccaccca
   101821 gcagagttat tcccgccagc acacaaagaa caacagcctc ctgccgtgtc aaaccaaaat
   101881 attcaaaaaa tttataggcg tttgcgcccc agttcctaaa attcatcccc caattccccc
   101941 ggcaatggca taattatgcc gtaaatgctt cctttatttt tttaaagaaa ctcgtgtcgt
   102001 tactattcgg cgatctaaaa ttatcataac ccataagttc ttcgataagt cggcgttctt
   102061 tgtcgctgat cttggatggt gtgtgtacgg cgacacgaat caattgatct ccggaaccgg
   102121 agccattgag atgcggaatg cccttgccgc gcatgcgcag tattttgccg gattgaatac
   102181 ctcccggaat cgttaacttg gcttttccaa atagggtagg cacttccact tcatccccta
   102241 aaaccgcttg agggaacgtg atatttacgt tgagcaacac atcatcaccg tgccgttcga
   102301 aaatttcatc ttcaatttct tcgagaacga caatcacatc gcccgattca cctccatgcg
   102361 gtgcggcatg accttgcccc cggagcggaa tataatttcc tgtagttaca ccggcgggaa
   102421 ctttgacgct gatcaccgtt tcacccatgg tcactccgtc acccttgcag gttgagcagg
   102481 gtttttcaac gatagttccc tggccttcgc aattgcggca cgcagcaata ttcacaaatt
   102541 ggccgaatac cgatcgcgaa acttgtctta cctgacccga accgtgacac acactacacg
   102601 tttttcgcga tgatccggcg gctgcaccgc ttccgttaca tgattcacag gacttgcgtt
   102661 tacgaagttt tatcttttta gtaacccctt cggcgatctc ttgcagtgtc agtttcaact
   102721 taacttccag gtcagcgccg cggttggaac ggctactgcg cccgcgccca ccgccaaatg
   102781 catcaccgaa accaaagcct tcgaaaatat cgctaaagtg tgaaaaaata tcattgatgt
   102841 cgctgaatcc ttgctgaccc atgccgcgca taccgtcatg accaaaacga tcatattgag
   102901 cacgcttttg ttcatcgctc aacacggaat acgcttccgc aatctcttta aatttttctt
   102961 ctgcggactt atccccggga tttttatccg gatggtattg catcgccaat ttgcggtacg
   103021 atttcttgat ttcatccaac gaagcgtttt tggaaacact cagcacttcg tagtagtcgc
   103081 gtttagccat attcctttat tcaaaaaaat gaatgttaaa ttcgggtata taaaacttac
   103141 atgcatcgta agttttcaga aataaagtct ccgtaagaac cggttattag attggaagtt
   103201 gaaaacattt tcataagaca acaaccgaaa aaccgaatac cattactcca taataatatc
   103261 tatgatttta tcttcgttca gattaaaatg tttatccttc ttttctccat tttcttcgta
   103321 ctcgacagca aacaccttat tgtattcatc tttgagcgtg atgatgcctt tgacagaaac
   103381 accgcgacct ttgataacgt cgaaatatga aaccgcaata tgatcaccgt gatccaaggc
   103441 ttccagcaat tcgatgatct gggctacttc cataaacgta ttctgctgat tctcgtcgag
   103501 atcaatactg atggccacgc gatccaattt tactatatca ccgacttcaa tataaaactc
   103561 aatagattct ccggaaccct cctctacaag tacaaatgag gtgtaatccg tcgccacgtg
   103621 acttacgcgc ccggaaacag tcgaacggct cccgtcccaa tccacacgtt cgacggtagc
   103681 gccatcgttt acggctaacc ctttgatagc cgctttgacc gcatccgcat ccaaggcacc
   103741 gggacgcgca tgtatttttt tctttccaaa aagcatattt caaaaacctc cgttataaag
   103801 tacaatcatc tgacttctaa actgcttcgt gcgccatttc cacaaatgct aactcgccat
   103861 cacgcaactc gatagataca aggctcccgt ccgaaaactc accttttaat attttttcag
   103921 caacagcatc ttcgatatgt ttctggattg ctctgcgaag cggtcgcgca ccatacaccg
   103981 gatcaaaccc tttcgtagcg acaaattctt ttgccgcatc ggtcaaatgt aaagtcatat
   104041 tgcgttcttt gagtttctta cgcacttcat ccagcgatac gtcaataatc cgcaacacat
   104101 tatccttggt cagtgcgcgg aatacaatga cctcgtcaat acgattgaga aactcagggt
   104161 taaaaagttt tttagttgct tcttcggctt tggctttcat gcgcagatat tcatcttcac
   104221 cgttatcttt tgtcgaaaaa ccgaacccat caattttact catttcgcgg gcaccgacat
   104281 tggatgtcat gataatcacg gtatttttga agctcacttt gcggccaaga ctgtccgtga
   104341 ggatgccgtc atctagaact tgtaggagaa tattaaaaac atccggatgg gctttttcga
   104401 tctcatccat aagaacgacg ctgtacggtt tgcggcgaat tttttccgta agctgaccgc
   104461 cttcttcgta gccgacatat cccgggggcg gaccgactaa acggctcacg gaaaattttt
   104521 ccatatattc cgacatatca atacgcacca gcgcatcggc attttcaaaa agataataag
   104581 ccaattcttt ggcaaattgt gtttttccca caccggtcgg accgaggaaa ataaacgaac
   104641 cgataggacg attagggtct ttgagtcctg ctcgtgtacg acggatggcg cgtgttaccg
   104701 actgtatcgc atcatcttga cctatgatac gactgcacag aacattttcc attttgagta
   104761 atttttctgt ctcactttgc gcaacacgat tgacaggaat acccgtcatc atggcaacga
   104821 cgtgcgcgat ctgctcttca ccgacttcga cgacttgcgt atcctctttc ttttcccatt
   104881 cctctttcgc cacgcgaagt tgttccgtta atcgtttttc gacgtcacgc aactcagcgg
   104941 ctttttcaaa tttttgattt tttataacaa aatctttttc cgtacgaacg cgctctattt
   105001 cctgttcaag cgcaacgatt tcttccggca cgacgatatt cataatgtgc gtgcgggaac
   105061 ctgcttcatc catcacatca atggctttgt cgggaagatg acgatccgtg atgtaacgat
   105121 ccgacaaacg aacggcttgc accagcgctt cgtccgtgta cgtgacgcga tgatgttctt
   105181 cgtatttgtg ttttatttgg cgcaaaattt caagggtttc ttcgaccgat gggggatcaa
   105241 cgatcacttt ttgaaagcgg cgctcaagtg caccgtcttt ttcaatatac tgacgatatt
   105301 catccagtgt ggtagcgccg atacactgga gctcgccgcg ggacaacgca ggcttgaaca
   105361 tgtttgatgc gtccaaagag ccgcttgctc caccggcccc gacgatcgta tgcagttcgt
   105421 ctataaataa tatcacttct gtggttttct ccagttcact catgatcgct ttgacgcgct
   105481 cttcaaactg gccgcggtat tttgtaccgg ccaccagcgc gccgacatcc agagtcacaa
   105541 ttcgtttacc caatagaaca cggggaactt ttttctgaac gatacgcaaa gccagccctt
   105601 cggcgatggc tgttttcccc acgccgggct ctccgatcaa aacaggatta ttcttttttc
   105661 tgcgtgaaag gatctgtgca acacgttcga tttcttcatc acgaccgatg atcgggtcaa
   105721 ggcgattttc ttcggcatac tgcgtgagat cgcgaccaaa gtgatccaag gcaggcgttt
   105781 tacttttttt aggctccggc tttttggctg taggatttgt aggagtctgc gttccgctca
   105841 tgatattggt cagctccctt cttaccgttt cgtaatcaat attgaacatg gataaaactt
   105901 gcgcagcaag gcaatctttt tctttgatca gcgcaagtaa aagatgttcg gtaccgataa
   105961 catccgattt aaaatttttg gcttccacat acgtggattt caagattttt tcggctcgct
   106021 tggtaagcgg cggattaccg atgaccgtat tatccgttac cggttttacc atatcttcga
   106081 tagcacgttt cagtttttct aaatccatgg ccaaattttt gagcactttg acggcgacgc
   106141 cttcgccttc gcgaatcaaa cccaacaaaa gatgctccgt acctatataa tcgtggccga
   106201 gccggatggc ttcttcctgc gataatttta cgaccagttg gacacggctg gaaaaattat
   106261 ttttcatact gactccattc tgaaaaggcc aaataacaaa acttgttatc gttattttgt
   106321 gcacaattca aaaaaatata gagattcggg ctacattagt caagaagtaa taatgttata
   106381 aaaattacac acttagacgc gagaaaaacc aatcataaaa caacacaaaa agccgattaa
   106441 tttgaaatca gggtatcaga aaaagattaa taaaattgtt ttaattaacc caaatagtgc
   106501 cgtaatacat gacttcgcga agtgtgacgc agacggcgaa tcgctttttc tttgatttga
   106561 cggacacgct cacgcgttaa ctgaaaacgc acacctattt cttccaatgt catcggatga
   106621 tcattttcga gaccaaagta caaacgtatc acttccgact cgcgcgcgga taaactgtcg
   106681 agcgcacgtt cgatttcaat tttcaaagac tcgttgacga gatcgtgatc cggcggcgcc
   106741 tgctgctcgt tttcgatcac atcaatcagc gtattattat ctgaatcgta aatcggttca
   106801 tcaaccgaga tatgccgccc ggacatttgc aatgtatcgt gcacctcgag ttcggaaatt
   106861 tccaactgct ccgcgatctc ttcagcgctg ggttcgcgct cgaattcctg taccaaatca
   106921 ctatacacgc gaccgatttt attaagagtt ccgattttat tgagcgggat acgaacgacg
   106981 cgagattgtt cggcaagcgc ctgaagtatg gcctgacgaa tccaccatac ggcataagaa
   107041 ataaatttga atccgcgcgt ttcatcaaat cgtttcgcgg ctttgatcag tccgatatta
   107101 ccttcgttga taaggtcatt gagtgagagg ccttggcctt gatattgttt ggctacgctg
   107161 acgacaaaac ggagattggc tttggtcaga tgttcgagcg cccgttcatc accctcgcgt
   107221 atgcgctgtg ccagcgcgat ctcttcttcg gatgtcagga gcggtacggc gccgatttcc
   107281 tgcagatact tgtcaatgag atcattattg tgttcgacat aatgagccat agacaacctt
   107341 ctttcatttt gttgtacgat ggttaacgat ctatacggtc tacaattcct ataattgttg
   107401 catcagtagg cgctttgcga tccagaggga tcggcgcttc acccgatgtt acataataaa
   107461 ctaattcatt aggctgtccg gcgcctaccg agtcggtcgc cacgatcggg ccgccgacgt
   107521 gcttcaattc tgaatccacc ggctggatga tctgtaaacg acacccttcg atagaaggat
   107581 gttttttagt agcccaaacc gtgccgatga tacgcgccat attcatatat gctgcaaatt
   107641 ctcgcgtgtg cgttcgtttt tgatattccc tgtatttacg gtcgttgccg gttcaaaaag
   107701 catgatctcc gtttctacgt cagaaaccgg ccggtgttcc actccgcgcg gtacgatgat
   107761 aaattcaccc ggatcaatga tttccgttcg atcgcgaaat tccattttca aacaaccgcg
   107821 aaccacataa aacatttcgt cctcatgatc atgatgatgc caaacaaatt caccggaaac
   107881 tttgactaac ttgacatgct gaccattcag ttcgcccacg atacgcggat gccatgtatc
   107941 ggaaaaagtt tcaaacttat ccgcgagatt aattttattt gcgatcaagg atgaacctcc
   108001 attttatcta caatagccat aataaccgca tcaaccggcc gattttctgt cgcttcggtt
   108061 tgacgtgccg aactgccggt acaatataac accacttcac cgacgccggc gcctacagaa
   108121 tccgccgcca ccacgaaagc atctttttct ttgtagtcca atcctatgct gcgcacaatc
   108181 aacagcttca cgcccgtgag tttttggtct ttccgtgtgg cccatacaga acctataact
   108241 ttacctagaa ccatatattc ctaatctgcg catgcaatgc ggcgctcatt aagtttttta
   108301 gcattcacta aagcaacatt agcacgatgc atcagtgcgt cggcttcggt accgtctgta
   108361 ccatatgtaa ccacaccgac cgttgcggtc aacggaatct cttcatcgcc ggaatgaatc
   108421 accgccgatt cgagcgcgtg gagtatccgg cctgcacatt ctaccgcttc tttcctttta
   108481 ctttctacaa gtaacacccc aaatcggtcg tgactcaaac gagcaaggct atcaataaac
   108541 cgtatcccat tttttaaaat ttctgttatt ttttcaagcg ctaaatcacc ggcttctaca
   108601 ccgaaagctt cataaatatt cttgacgtaa tccacctgaa aaatcagaac actaaacgaa
   108661 gtttcaaatc ttttagcgcg atgaatctcg gacacgacgt ttttcataaa cgtttgctcc
   108721 accgttgacg catttcgttc gccttcgttt gcatttttag ataaatgctg atccagagcg
   108781 atacgcgcca atgcgagccc tgcgctattg cagaccgttt caagaaactg cacttcatac
   108841 acatcgtatg catttttttg gccttgttcg atcaccagca caccaaaaca atggtcctga
   108901 tcacagatcg ggatgaccac gacggaagca aggtaattca attccaattc gctgtttacc
   108961 gaagtccggt caatggatgt cacatcagat atataaagcc gacgacgttt cgtcataact
   109021 ttttcgatca tcgtgccgtc gagccgaacc atagattctt tatcgtatgc cgcactgcca
   109081 ccggcctcca ccaatcgtat ttcctccgcg ccatgtcgca accatatatg catgcgatca
   109141 aaacgaagcg tgttttgaat ctgttctttc aggcaagccg tcgtttgcgt ttccataccc
   109201 gcaaggctca ggcgctcgct aaattcacgt aaactctggg aaaggcgacg gagcttgttt
   109261 ttcatataca cgccgtcaat actttgaata gacaaaagta tgaggcgtcc
//
LOCUS       DAOQGF010000003        73467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_105_length_73467_cov_7.781262, whole genome shotgun sequence.
ACCESSION   DAOQGF010000003 DAOQGF010000000
VERSION     DAOQGF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 73467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73467
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_73467_cov_7.781262"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1256
                     /locus_tag="PK937_01215"
     CDS             12..1256
                     /locus_tag="PK937_01215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNH31225.1"
                     /translation="MNILVLNSGSSSVKFQVINTDLTQIRDNADTRLASGIIERIGSE
                     SIISFKAEGQPTLKQTAPMRDHKMAVDKIVKWIIGKDTKINGINGLSDIHAVGHRVVH
                     GGEKFTRSILLNEQIIQEIEDCIELAPLHNPANLKGIYAVTEIFGAGIPQVAVFDTAF
                     HHTIPEEAYLYAIPYQFYRRYKVRRYGFHGTSHRYVAYRYRTLTGQAKEQVHIMTLHL
                     GNGASICAIRGGKSVDTSMGLTPLEGLVMGTRSGDIDPAVLEFLSHKEGYSLQEIESV
                     LNKQSGLLGISGLTNDMRDIIAEAKENDDRRAKLAIEIYCYRIRKYIGAYCAAVGKPE
                     ALVFAGGIGENSAYIRESICKGMEFFGLTLDPHLNAATVGGKEGVISSPDARLKTWVI
                     PTNEELLIARDTVRCVEKAPLP"
     gene            1457..2542
                     /locus_tag="PK937_01220"
     CDS             1457..2542
                     /locus_tag="PK937_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH31226.1"
                     /translation="MNYILREAWDVLKNPGIIPKLPNMMRASASYALNKWTRKNIVWG
                     YPFVMMVEPTNICNLRCPLCITGSKLMTRDDGMMTLERFKQLMDEMGPYLMHLTLWSQ
                     GEPFVNRDFVKMIRYAHDKHIKTVTSTNGHFLSENAEAIVESGLDTLIIAVDGASQDT
                     YEKYRVNGKFEKVFNGMKAIAAAKTKLKRKTPAIELQFIVMKHNEHELETIRQMAHEC
                     HVQVLSLKTAQVYTAEQALEFLPNNEKYRRYEIGNDGTFAAKIGSINFCRWVLMCPVI
                     NWDGTVSPCCFDKNADYPLGNVFDPGGMKTIWKNKAYHDFRRRIFTQRSAIPICSNCS
                     EGLEVEVYEKELIPSRPASAVALDHVA"
     gene            2558..3397
                     /locus_tag="PK937_01225"
     CDS             2558..3397
                     /locus_tag="PK937_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31227.1"
                     /translation="MSKKKEKKKSEKKQGKKKPVKPVSVKKSTGKKAGSKPASKKAKP
                     TKKSVAAKSPSKNTAPSVDKKQASAKSTKKQPSRPPIPRTPADNVRRPFNIIESYMGE
                     YRFNDMFAILFVCTGNMCRSPLAEGIFKRKLAIECPESIKHKIWVESCGIYAYDGNKP
                     SENAIKVGHQNGVDISAIRSKPINRVLVEQSDLIFALSIDHLNYIQENFPTARHKTYL
                     MKVFGKDRGVTISDSIPDPMGFSMDFYLKTYNEIHVTIDEIFQKVVAMAEQKLNPKSS
                     ESM"
     gene            3397..4149
                     /locus_tag="PK937_01230"
     CDS             3397..4149
                     /locus_tag="PK937_01230"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNH31228.1"
                     /translation="MRFAIVFLFFYISGIAHVTAGDPLRTLPNKAFPVGEKLKFSIGW
                     EFISAGTATLEVEQLTAHRDRPCYLVSATTVSNAFFSTLYPVKDRLESYIDQNGLYPL
                     KYVKNTSEGGYKRNFTTEFYHEQRKATIDDRDSGYKEIAIPEMVQDIISAFYYLRNQS
                     LEIGKTLSLATYDNGKINQATVKVVRKETISVDAGEFHCILVQTPIGPFNNKSDLNIW
                     LTDDDRKIPVLMKSKIAIGSVRAELVEMSGTF"
     gene            4193..4906
                     /locus_tag="PK937_01235"
     CDS             4193..4906
                     /locus_tag="PK937_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNH31229.1"
                     /translation="MQHYVHGWYSERLGKQMNFQHFGHGGAPVVVFPTTLGNLYEFSD
                     RRMIDPLAEKIENGLIQILCVDSINNESWYNDQIHPHEKVRRHVLYEDYLIHEFLPYL
                     RNKTGTDYLILLGCSFGGYHAVNFAMRHPEMVNRAIGLSGSYTIQGFLGGYYDDLCYY
                     NNPAHYVQHLSDPYYIDRYNTHTEITLVTSDLDPCRERNEYFHQILSEKGIRHNYFFW
                     DGGIGHDWPYWQRMIGHYV"
     gene            4928..5635
                     /locus_tag="PK937_01240"
     CDS             4928..5635
                     /locus_tag="PK937_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31230.1"
                     /translation="MIGIRLLLFISVLFFFYFLARQPDTAETMRCDASFYGHGRVPVK
                     LRRPVNDSIPATPIRVAQFFRWTLSQDALIQHMDVLPESEPVTGFEDRAVCIEGYAVA
                     IRYDHKDGLYQKDNALYLEMTDSPRWDTPHLIVRIPPGEAYCDARTTLWNIAHWDASA
                     AKEKISAGLRLMRRPTRVRVTGFLYINPETKKKYDRHGISYQTKGIPDGIQRKRTPPQ
                     TQGVWEIQPAFRVELIN"
     gene            5644..7128
                     /gene="glgA"
                     /locus_tag="PK937_01245"
     CDS             5644..7128
                     /gene="glgA"
                     /locus_tag="PK937_01245"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNH31231.1"
                     /translation="MKTPLKIALASSEVAPFAKTGGLADVSGALPKYLTRLGCDVKVF
                     MPKYVSVDDSKWDLHYCADIGEILIRMGRVTRSAHVLKSVIPGSEAEIYFIECPHYYH
                     RRHIYTMDRDEDERFILFCKAVIEVMQRIQWAPDVVHCNDWQTGLIPIYLKDNYNWDR
                     MFDRTACLYSIHNIAYQGRFSKDTLYRAEMRPDQFYPGGPMEFHDTFSFMKTGILSSE
                     IVTTVSHTYAKEILTYEYGNGMNDVLWPRQNDLFGVLNGIDYDEWNPEKDKYIPHPFS
                     QSDLSGKEKNKKFLLEQTHLTYDPAKPVIGIVSRLVGQKGFDLIEKALPELMLLDAQW
                     VILGSGEDRYEDMFENLHHALPYKVWTYIGYSNELAHRIEAGADIFLMPSHFEPCGLN
                     QMYSQRYGTIPVVRKTGGLADTVWDWHELHSAGRGIGNGFTFTDASPYALFTTLLRAL
                     ETYKDKTVWRKIQQNAMAMDHSWDASAQRYIELYKMAIQKRRAE"
     gene            7306..9117
                     /locus_tag="PK937_01250"
     CDS             7306..9117
                     /locus_tag="PK937_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNH31232.1"
                     /translation="MRLKIKYLDGIRLKRSVIAGCNHVINKKEYLNNINVFPVPDGDT
                     GTNMASTLKNIAETVEKEELHEIDSMAIVLADSALMGARGNSGVIMAQFFQGMNEGLQ
                     GSAKCTTKKFSDAVQKATKSAWSAISNPVEGTILSVIRDWGKSVEKNAEKTDDFSELF
                     RESLADAKQSLANTPKQMAALAKAGVVDAGAQGFVTMLEGVLDFIENGRISDIKRQGL
                     SETEDEAMEAHIDEHAFEEITYQFCTECLLVGENMDREKIRESLNKFGDSLIVAGSTK
                     KVKIHLHSNTPEDVFEVLADHGHVISTKIDDMKQQFLNAHSENVQETAIAIDSCCDLP
                     LEYIRRNHIHVIPLNVHFGEQIYIDRLTLTPKKFYEKLTSSPDHPKSSQPTPAKFKES
                     YERLLQIYKNVVSIHLSSKLSGTYQGALSMARQVAQDKIFCFDSMSASVGIGVMVREI
                     EPMIRENAPAEKIMQHLEYCRNNMKVFISVQTMDYLIKGGRVSKARGAIAKLLNIRPV
                     LTVQDGAVVPIAKAFGHSNALRKVMDLVKATTKNRKKPRFGIAHANSIGIADWYATQL
                     SRYYEIDRDDILITDVSTVIATHAGPGAAAIAVLSDP"
     gene            9147..9641
                     /locus_tag="PK937_01255"
     CDS             9147..9641
                     /locus_tag="PK937_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNH31233.1"
                     /translation="MPRGPYEKWIEVICISVVVASFVLVMGHIQELPESIAIHFDWSG
                     YPDNWGSKHTLWLLPALSALVIYMPLTVFSFLQQGHTKPDDISSTLIHRNFKIARISL
                     ALIKLNVLFLMWWLTYIVIQSALSGRIDHNPVGVLSIFLLILTLIIGISIYLVKAKRS
                     KPSA"
     gene            9634..10350
                     /locus_tag="PK937_01260"
     CDS             9634..10350
                     /locus_tag="PK937_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNH31234.1"
                     /translation="MHNWIIIFLLLHVIPVSSQTMSDPGLKKYIGLWEREQTDSLKTL
                     IAEMEKKFPKAPETVFLKNTYETDGAIAYAVYLRFVRDYPKNIFAPEAYHRMIQYDAA
                     AGRYALAQDNLIKLRTDYPGSSCIKSAETLFPATKPDEATQAIMTAPSDTARPTEINA
                     GKKFRLQVGAFSDQKNAQELKQKLTAKGYAPIEFSEKTVNGKTLILVWVGAYTTHDDA
                     KKAGDTIKSKEQLNYSIVEK"
     gene            10340..10909
                     /gene="thpR"
                     /locus_tag="PK937_01265"
     CDS             10340..10909
                     /gene="thpR"
                     /locus_tag="PK937_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNH31235.1"
                     /translation="MKNETIRAFIALDFPNEAFSFFSEQERRLKPLFNDARIAWVNPT
                     NIHITLAFLGEINRDAIPKITRCLSDLDEAGPFYLAATHLGFFSTKGNPKVLWIGFKK
                     TSSIESLQNKICIRLNALGLTTEEKKIIPHATLARLRDWPSARLAQQNVIHQFIGETE
                     WVMPTLTLYESVLQRQGAQYKPLFQIKFT"
     gene            10906..11595
                     /locus_tag="PK937_01270"
     CDS             10906..11595
                     /locus_tag="PK937_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNH31236.1"
                     /translation="MKIASIQQKKVKRNQSATVCVLTEEGDSFELLYETLVKFGIYDG
                     SDLDEKKWNRIQLWDSTRRATTDALRWLSLRPRSREEISNRLRREGYNDETITSVLEK
                     LSEEKYIDDLDFAMRFAQSRLRKKAMGERALAYELNKKGIAPDLIRQVIQKFVPENDL
                     PSVLRLAEKKYATIRESDQRKKQIKLSAFLQQRGFDTDTIRKVVETIASHEKIISRDQ
                     NPLIPSNFKKH"
     gene            11755..12651
                     /gene="gnd"
                     /locus_tag="PK937_01275"
     CDS             11755..12651
                     /gene="gnd"
                     /locus_tag="PK937_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNH31237.1"
                     /translation="MQLGFIGLGKMGGFMVQRLMRDKHEIVGYDLNPDVVKGLTEKGM
                     TGASSYEDLVGKLKGRKAVWIMVPAGKPTEDTVAKLANILDKGDIIIDGGNSYYKDDV
                     RRAKELEAKGICYVDVGTSGGVWGLQNGYCMMVGGNKADVDFLSPILTTLAPPNNGWA
                     HMGAHGSGHMVKMVHNGIEYGMMQAYAEGFEIMKASEYKLDIARIADLWMQGSVVRSW
                     LLELTAEGLKKNPTLDGIKGWVADSGEGRWTILEAMEKDVPAPIITLSLFERFHSRQP
                     ESFAAKMLAMMRNQFGGHEVKK"
     gene            12729..13247
                     /locus_tag="PK937_01280"
     CDS             12729..13247
                     /locus_tag="PK937_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31238.1"
                     /translation="MDKVIIVRAVPEDAEKLTHISRQTFYETFSEQNTKENMQKYLDE
                     SFSIERLTDEMHDAHSQFYFAKSGHDIVGYIKINLRPSSAEIKNIYSLEIERIYVRHD
                     FQGKKVGLSLYEKAMHIARESKADHVWLGVWEKNYNAITFYNKLGFVAFGSHPFRLGD
                     DIQTDILMKKQL"
     gene            13308..13871
                     /locus_tag="PK937_01285"
     CDS             13308..13871
                     /locus_tag="PK937_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNH31239.1"
                     /translation="MNAFKKISKTQCTSLIETLKIRFEKNRIRHTAIKWTDVSVKIEN
                     KTTKLFSLWKMEETGGEPDVIGFDIKTGEYIFCDCAPESPAGRRSLCYDKDALDARKE
                     HKPKDNAMDVAARMGIDILTEEAYRELQKLGEFDTKTSSWIKTPDQIRKLGGALFCDR
                     RYDHVFVYHNGAESYYAARGFRGMLKI"
     gene            14016..14660
                     /locus_tag="PK937_01290"
     CDS             14016..14660
                     /locus_tag="PK937_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31240.1"
                     /translation="MIETENAQTYVLKMRGSGNGAISLVSEFIVSRIASQLGWPIPDA
                     QWIQIPDNLPWTFGSDEFDDIVQKSFGWNLGISYIPDSFPITFDNQLLTDPVLVSALY
                     TIDMFFMNMDRTNSANNLLRDIHNKIWIIDHGALALFQPGVKQTLGLFHNHILFEIPS
                     ISKKQYNTALHTPELFKTIIHQIPDAIINETHFSKETLLARIINRTVAMSEFVV"
     gene            15054..16685
                     /locus_tag="PK937_01295"
     CDS             15054..16685
                     /locus_tag="PK937_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNH31241.1"
                     /translation="MRNLKITYKLLIGFGTLLAMLIGLSYFAISKMSDLFGMSKAAID
                     VIPAQNAIVEIQYHTTKGHLWFEEMLSGDNSIRMEEVYDHWNGGIEYASVLLDGGTRD
                     NQTYSAVKDTALIEILTSIRYALVDMVNAADERLELKKQKIKSEAGTESDIYFDAAYQ
                     RVMDYCDNGKKITTGLINKSVRNLENDFSSSRWTLIIETTVIVLIGILFSTLIARTIA
                     LPVREAALKINRDGVNTRFDMQRKDEIGQLVQTVDSFLSSFRMILREVADASAAVSSS
                     SKQIGVSTENMARNAQDQMGQTASVTAAIEEMSKTSYEIAHNIGNTKEMAIHAQSSAD
                     KGSQVIDETVDGMQKMLAMVQQSSNVVNSLSESSRKITKVVSIIDDITKQVNLIALNA
                     SIEATRAGEKGKGFAVVADEIKKLAERTAHSTTEINDIISKIQVNVNEAVNLMGRSNE
                     EAANGIRLSQEAKSSLKEISDVFKNVTMMVSQVAVSSQEQSVTGDQITDNVKVINTAT
                     QQIAGGINEIAKSAEYLNNLTTTLQKTVKRFKIGD"
     gene            16678..17337
                     /locus_tag="PK937_01300"
     CDS             16678..17337
                     /locus_tag="PK937_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNH31242.1"
                     /translation="MIKNIHQILFDFDYTLADSSEGIITCVNFALEKVGAPPGQPDAI
                     RRMIGHSLENTFARFIDPSDTEKIQTCKSLFMEHADTGAMVRNTVMLDGVRETLEFLY
                     NELYTLGIVSTKRRSTIWETLIEKEMDDFFDIVIGYEDVTHLKPHPEGLIKAIEELAG
                     TPETTIYIGDSPLDIKAAKSANVFVVAVTSGMHSTDELATLNPDLIIPRLSDLKAHLA
                     A"
     gene            17364..17834
                     /locus_tag="PK937_01305"
     CDS             17364..17834
                     /locus_tag="PK937_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNH31243.1"
                     /translation="MNFILSCGAHNVKQTASVEPSPIIQPISQQDHSEKAFDFTMLND
                     PWIIEPRTYEVTIQPYTEPAEVVVTSSGKETSVIGYRVQLISTTDYYEALEIRDSVSV
                     RLSGEIYLDYEPPYYKVRAGNFIQRDDAEMKKAEAKQAGYPDAWIVRTKIIQKK"
     gene            17862..18767
                     /locus_tag="PK937_01310"
     CDS             17862..18767
                     /locus_tag="PK937_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNH31244.1"
                     /translation="MEVKDIKTVGVLGCGLMGAGIAQVAAASGFKTIVREVNQDVIDK
                     GVGNIKKQLERNAAKGKITAEEKDKTIANLTGTTSLDDLKNCDFIIEAIIEDVKIKNE
                     TYAYLDKICPPHTIFASNTSSLTVTEMAAATNRPDRFCGMHFFNPVPVMKLVEIVKAV
                     RTSEETFNRAFELGKAFGKTPIATKDNSGFVVNFLLVPYLLGAIRAVQNNIASIEDID
                     KGMMLGCGYPMGPLTLLDFVGIDTTYRIAEIMFKEYGEPMYSCPPLLRKMFLAGFYGR
                     KNGKGFYDYSSGTPVVNKDVMNFVK"
     gene            18922..19851
                     /locus_tag="PK937_01315"
     CDS             18922..19851
                     /locus_tag="PK937_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNH31245.1"
                     /translation="MKKLFLTLTLLAAFTVSATDNTAASGSVVAVKEFTRSFPVSPGG
                     QLTLTTDFGSIKIVGSSDDKVSIMARMRGSDDDLKDFEITAEKTGNDVTIRGNWMRKN
                     NFFGHNSNSMDVDYIVNVPSQYNVSLGTAGGNIVIQDVKGLIQGGTSGGNLDLTNING
                     NAELKTSGGNIRAKSTTGNLNMRTSGGNVSMSNINGDLDVSTSGGSIRIEDINGRTNA
                     ETSGGHIEVYVSGNNKGVNVETSGGHIALYLPKNIAATIDASTSGGEVEFDLPIMVNG
                     KMRENKVRGTINGGGELIRAHTSGGDIRILTRN"
     gene            complement(20014..21009)
                     /locus_tag="PK937_01320"
     CDS             complement(20014..21009)
                     /locus_tag="PK937_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH31246.1"
                     /translation="MKIKNYRLIIHGGIISAFLLYSFTLSAQVRPLDQAILRPGSGVY
                     SDLAHIEGETIKDMVAALSADSMEGRATGTRGFDKAARWVKEFFVQHGIQPLPGGYFQ
                     DFTVSTSVLKERLHSNEWSSETASTKNIIGYIAGNDSLRRNEFIVLTAHLDHLGKEGD
                     SIYAGANDNASGVASVLQIARWIKESKTDLGRSVVFILFSGEEAGLLGSYHFIENPWI
                     DLNKIRLLINLDLVGSGRDGIMLQGSENYPDYATRIKKINDERFGFEMSTRPNSPNSD
                     HYYFNKIGLPAFFVYAYRGTMPYHAPGDTSEKIDEKVLENVTRWVAAMVVAFGNE"
     gene            complement(21013..22344)
                     /locus_tag="PK937_01325"
     CDS             complement(21013..22344)
                     /locus_tag="PK937_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNH31247.1"
                     /translation="MKFLSYGISLFFYSFILLAQNENNAVRPFMNEFGPGARALGLGG
                     AYSAIAEDYTAVYWNPAGLAQIRKMEFYTAFAHRNNTNAITYQGTRTETSNGFTSMNA
                     LGFVFPVPTTRGSLVLAFGFHRVNSFDDYNRVTGSPMIGNISFDQDEKTTNTGSLNHW
                     SFAGALDLTRNVSVGATLNIVTGKNDLSVDFFQDDEIAMLDDSIATKEVSFAEKPDYS
                     GFNFKIGTLFRPAKNLRVALTVTAPTKITVSENYSESTTIKTDNNGYYSDSWYEPYKY
                     HITSPWKFEIGSSYKYKLALFTGSVEWVDWQQTRFSSNIFDADGKNIDDAINNALLND
                     YRTSVNYRIGTEMVVPQIGCKVMAGYAYQQSPYKNGLEIVNSDHRYISVGASFLLDEQ
                     VKIDAAYQYGWWKQATTDNLLAYGPSPDYYNRYTSEKITSKKLLLSLSYRF"
     gene            complement(22359..22955)
                     /locus_tag="PK937_01330"
     CDS             complement(22359..22955)
                     /locus_tag="PK937_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31248.1"
                     /translation="MKYVGLLITILVSLYGSACYTVVGISRSDDRIQNEATESEPDFA
                     AYEPYGVTPSTMWEYYYETPFPWWYVPNADEYDAFDDTTTATDPTETSSYSSSRRNYG
                     RRRAALNDASSGSSVSTGYASPVYSPGGTTPSFVPSYSTGSSASYSSPSTPVKTDSTV
                     QSGSGSSDSNSNSTTKTQDTNRKRGYGNRRTAKSGDKK"
     gene            complement(22959..23867)
                     /locus_tag="PK937_01335"
     CDS             complement(22959..23867)
                     /locus_tag="PK937_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31249.1"
                     /translation="MKASFKKILTVLVFSLIWSGCYTAVRQSGGYYSEFDHADAKQPE
                     ITQIQPSDEDSSDQDEEVTPSYDEDRDIYIQNYYGPEYPYPYGYWTPGWVNYRHPYYY
                     GAGMYFGPRVRLAYVGTYWGYGYYDPFYDPYYYYDGWCYYPTGYGHHDNNPIHPSQQR
                     TYGGRRVYTASSTPNLPGIVGVSVASSGGGTTQRIYRSGNRTSGESRIDQDRVRRNYR
                     KYYGFETGDKAVNAEAGRKSRNANRETSTRNKETRETRQVRDNNSRNNGGERNARSGE
                     RNSGRSGRSEGRSGETRSSGKRSARN"
     gene            complement(24063..24785)
                     /locus_tag="PK937_01340"
     CDS             complement(24063..24785)
                     /locus_tag="PK937_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNH31250.1"
                     /translation="MSDAFLRAQWKNLIMANYAVDPSLLRPYVPSGTELDSWKDTFYV
                     SLVGFLFEDTRVHGLSFPFHRTFEEVNLRFYVRRFESGVWRRGVVFIKEIVPRSLITC
                     IANTVYHEHYATHAMRHRFTSDAHCKTFEYSFKSANGWNTISAKTNTESIAMAEGSEQ
                     EFIAEHYWGYTRINEKKLGAYEVRHPRWDIYPVMDYHIACDIGGLYGEPFAKTMARNP
                     DSVFVAKGSPIEVMKKTVIEHI"
     gene            complement(24851..26398)
                     /locus_tag="PK937_01345"
     CDS             complement(24851..26398)
                     /locus_tag="PK937_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNH31251.1"
                     /translation="MLAQILSATTFGIDAFIIKVEAHLEGAVPGFITVGLPDNAVRES
                     KERVNAAIKNSGFTFPIKRITINLAPADLRKEGSGFDLPIAVGILAAHGQIESHRTEE
                     YVVVGELSLDGSLRPINGVLSLALRARREHIKGIIVPAGNAAEAAMAEGLEVIPVNAL
                     SDAVQFLNGQKHIAPHQVDMSALFDQAPAMPVDFRDVKGQEHAKRALEIAAAGGHNII
                     MIGPPGSGKTMLAKRIPTILPDLTPDEALETTKIHSVAGFLPQNTPLVTLRPFRSPHH
                     TISDAGLIGGGHIPKPGEVSLAHHGVLFLDELPEFKKNVLEVMRQPLEDGHVTISRAE
                     MSLTYPANFMLACAMNPCPCGFHTDPGNRCTCNPGMIQRYMAKISGPLLDRIDIHIDC
                     PAIKYKELSSEGHGETSSAIRERVKKARQIQLKRFESLRKKGQPVYCNADMESRAIRE
                     FCTIDARSQELMKLAITKLGLSARAYDRILKVARTIADCANAPAIRAEHVSEAIQYRS
                     LDRSYSG"
     gene            complement(26534..29791)
                     /locus_tag="PK937_01350"
     CDS             complement(26534..29791)
                     /locus_tag="PK937_01350"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNH31252.1"
                     /translation="MNHITRIILIVSCCMSFALEAQTKADSLKNISLSGLQFRSIGPA
                     FAGGRIVDIAVNPANPAEYFVASGHGSLWKTTNGGTTFAPVFDGQKSYSIGCVVMDPS
                     NSNIVWVGTGESNGQSNVIFGDGIYRSEDGGKSWKNMGLENSEHIGGIVIDPRNSNTV
                     YAAAYGPFRNDGGERGVYKTTDGGKTWTRVLHVSDYTGFWQIHMDPNNSSVLYAVAHQ
                     RFKKLYATVQGGPESAIYRSTDAGVSWQKVMSGLPSEDVGKIGLAIAPSNPQVLYAIV
                     QAKEKGGVYRSSDRGASWVKRSSYVSTYAFYFQELFCDPKDENTVYSMDVFNQISRDG
                     GATWSKLGEKYKHVDNHALWINPINTKHLIAGSDGGVYESLDQGKTWDFKANMPIAEV
                     YKVSIDQALPFYHVYAGTQDNNSFFGPSRTNSSAGVTNQDWTFTLGGDGFETQADWSD
                     ENILYVQSQNGGLVRYDKKTGEQLFIKPIDTADVGYRFDWDAALLISHHDPKRLYFGG
                     HRLFKTDDRGNTWTLLSGDLTRGVPQKLIKMQGKSWSYDELASKGSLANITAIAESPI
                     DPNILFVGSGDGLIHYSDDGGKTWNKPASTGGLPEFARVHTIVASSHNRRVAYAACHN
                     FGDGDNKPYLLKTSDGGKTWTLLNGNLPKRGSTYSFAEDHVDPNLLFVGTMFGLYVSN
                     TGGQDWVAFNNGIPPGLITDMTIQKRENDLVVATFGRGMYILDDYTPLRHLSKETLQK
                     DIAILPIKEASMFIPSNPFGFPGTGFQGAGFFATPNPETGAVFTYYVKDDYKSLKKKR
                     RDTEKEKQKKGEDIELPSYQTLRNEADEQEPFFVFTIKDELGQPVRRIKTSVQKGVNR
                     IVWDFRTNVFTPISLAPFDNSIPWNDPDRGYMVVPGKYTVSVSRYEAGQWTDLGVSQS
                     FICKPLLTPSLPVADRNKLDAFNKKVAELIRAISSADAYRESMVEKIPLLKQAVIETA
                     KLPEGTYAAVVAAEDKLKRIQRQMNGDGLRALYEGVPPTSLRGRVEIITGNLWTTTSD
                     ATTTFQHLYDVAAAQFDGLLRALQETDAEIKRIEQQMDRYGAPFTPGRLPSVIKN"
     gene            30123..30197
                     /locus_tag="PK937_01355"
     tRNA            30123..30197
                     /locus_tag="PK937_01355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30155..30157,aa:Asn,seq:gtt)
     gene            30265..31521
                     /locus_tag="PK937_01360"
     CDS             30265..31521
                     /locus_tag="PK937_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31253.1"
                     /translation="MRKSLLGFWALFIIPFSLYSQLTGFHLYEKDGVYNVSPWLRYDR
                     ASGFFTGLTGDYFFTERLQMHGEGGYAWAARKPDYHLRLQQSFISDNTEYSIAAAYFD
                     KADTRDRMVLPEWQNSLTSIVAKRDYYDWYRIKGGNFEMRTDISGTLKITISGTWQHY
                     RNADVNAQWSMFDWGAKKNNGHARFRINPEIPEGRDVSLGTAIELDTRPSPLASVSAW
                     LIHSDYANARLLHKAFNSDFSYQRGSLTITRQQRMFGKQKWTFYGAYDGFSGNTVIQK
                     DSSSSLTYGQFLYDAGGLGSLRGYGYKEFSDGNRRMLFQSEYYFNGSFLPQTPLTKIW
                     GLGFIFKTFDLMLFWDIGHAWQASEKVMYFVPDGFHWRDMRSSAGGGLAVSDWIKIQY
                     AYVLKDGIRTHRGNYGIYFQFTPKLK"
     gene            31527..32258
                     /locus_tag="PK937_01365"
     CDS             31527..32258
                     /locus_tag="PK937_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNH31254.1"
                     /translation="MIDSQYFFVRPQDVYADHLVLRDDESQHCVKVLRKAPGDRFYAI
                     DGLGHEFTVALEHSDRTSAHCRILSTASKPRELGFEITLVQALIKKDHFDLVVEKVTE
                     LGVHRIIPVKTRRSLPEAGHTKIERWHKIALTAMKQSRRSLMPEIMPVSDLNSVLRRY
                     ADRPVQKIFLHESSEQPLDPNGINFAKACDTIIVVGPEGGFTDEEAAMAAEYGFQSFS
                     LGLRRLRAETASICGVGLFSMFAHP"
     gene            32546..32824
                     /locus_tag="PK937_01370"
     CDS             32546..32824
                     /locus_tag="PK937_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNH31255.1"
                     /translation="MTKADLVAKMAKDADVSKTKAEKSLESFIEAISMSLKKGKAVTL
                     VGFGTFSVAKRKARTGRNPQTGATIKIAAAKVPKFKAGKALKHVVKKG"
     gene            32963..33550
                     /gene="purN"
                     /locus_tag="PK937_01375"
     CDS             32963..33550
                     /gene="purN"
                     /locus_tag="PK937_01375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNH31256.1"
                     /translation="MSKLILGILASGNGSNCRAIHKAITDGLCKAQIGVIISNHAEAG
                     VLQFARENGIPAFHIASEQFCEISEFHAAIKNCLSQHQVHLVVLAGYMKKIGKPILDI
                     YPNRIINIHPALLPAFGGKGMYGHHVHQAVLEYGAKITGVTVHFVDPEYDHGKIILQK
                     AVEVMEDDNVESLSARVLKAEHETYYKAINLFANE"
     gene            33600..34604
                     /locus_tag="PK937_01380"
     CDS             33600..34604
                     /locus_tag="PK937_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH31257.1"
                     /translation="MMKPIKNVLWLLIVGASLFAQGKTSIDSDKQKVAIIPIDAVKIA
                     ESYGATQTVNWQLSGATWKSAPESPFADPASLAAFAEAATQRVIEAFVKLGRFVVLDR
                     SAMEKIMKEQDFQLSDNVDPNTVTNIGGLLGAQYIVNGQLQQVSTNPYYNPKDKTTLM
                     GYSGTVEIQITILDVATGQVMTSKRVKGSTEVEGTKILGMALLDAYESTPIKAAYKGL
                     SECGSDLKKWLRDAFPVEGEIFEITKEDKKKGAIVVNITCGKDIGVKKGDRFRVYTES
                     EVEIAGKMRKKTTDVATLAVKTVAEDGWSSLCDVEKGGKSISEKVKAGEKLKVVSVKK
                     "
     gene            complement(34695..35273)
                     /locus_tag="PK937_01385"
     CDS             complement(34695..35273)
                     /locus_tag="PK937_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31258.1"
                     /translation="MSLRFPLFVFLVLLIASCGKKKTDESQLGQDSSSTAVQPINYDI
                     PIEAGRPVTSVKALPFREQLLAFSIELGNRSKDFNQRLGLILFAKRSGEPWREAVRNN
                     IARENYRNAELYHTAYLDSLRGVIRPTDAALKSSYNELLIAHKRWRNNYQILHDHEKF
                     ESMIAVLDTVLSNEMAINKALKDFKNTIDTKP"
     gene            complement(35270..37741)
                     /gene="leuS"
                     /locus_tag="PK937_01390"
     CDS             complement(35270..37741)
                     /gene="leuS"
                     /locus_tag="PK937_01390"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNH31259.1"
                     /translation="MSVTSTGNSEQPTAKYDFTKIEAKWQAYWDQHKTFRVTEDPKFP
                     KEKRFYVLDMFPYPSGAGLHVGHPEGYTATDILARYKRMKGFNVLHPMGWDAFGLPAE
                     QYAVKTGVHPAITTKQNIDTFRRQLKMFGFSYDWDREVNTTDPHYVKWTQWIFLQLFK
                     KNLAYIAEVPVNWCPELGTVLANEEVPEQIDKGFTVVRKPMRQWMLRITAYADRLLED
                     LNLVDWPESIKEMQRNWIGRSEGAEIHFRIKDHDGDIKVFTTRPDTIFGATYMVLAPE
                     HPLVEKITSAEQRNAVEAYRKQAALKSDMERTELAKDKTGVWTGAYAINPANGNPIPV
                     WIADYVLINYGTGAIMAVPAHDTRDWEFAKKFDLPIVEVVKAAKNVQEEVYDNNTDSV
                     CVNSSNAEVSINGMNYKTAFDTIVAWIENKGIGKRTINYKLRDWLFSRQRYWGEPFPI
                     VHLEDGRIMPLDESVLPLNLPEVQSYKPAGTGESPLATMTDWVNITMDGKSARRETNT
                     MPQWAGSCWYYLRYCDPHNDQAFVDAEKEKYWLPVDLYVGGGEHAVLHLLYARFWHKV
                     LFDLKLVSTPEPFMKLVNQGIILGEDSQKMSKSRGNVVNPDDVIAQYGADAMRLYEMF
                     MGPLTQTKPWSTRGVEGVYRFLQRTWRLFVDERDQLSSKVQDVEPTPDQLRVLHKTIK
                     KVQDDIETLSFNTAIAQMMIFVNELTPSDVRPRKVIEPFLLLLAPFAPHIAEELWSRL
                     GHTTSLTYEPFPVFDPAWTVEDTATVVLQVNGKLRDKLEVKKGLDKQTLETMAKEKLG
                     HWVGGKEIVKIVVVPDKLVNVVVKG"
     gene            38195..39430
                     /gene="icd"
                     /locus_tag="PK937_01395"
     CDS             38195..39430
                     /gene="icd"
                     /locus_tag="PK937_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNH31260.1"
                     /translation="MYKDITPPKEGQKISIQNGVLQVPDNPVIPFIEGDGTGRDIWRA
                     SVRVFDAAVQKAYGGKKKIHWFEVYAGQKSFDQYNTWLPDDTITACKEYLVSIKGPLT
                     TPVGGGIRSLNVALRQTLDLYVCLRPVRYFNGVPSPVKRPEKVDMVIFRENTEDIYAG
                     IEWKEGSPEAKKVIAWLQNEMGVKKVRFPETSGIGIKPVSREGSERLIRSAIQYAIAQ
                     KRKSLTLVHKGNIMKYTEGAFRDWGYELAKREFGGVEIDGGPWVKLPNGVIIKDAIAD
                     ITLQQVLTRPEDFDVIATMNLNGDYLSDALAAQVGGIGIAPGGNINYITGHAVFEATH
                     GTAPKYADLDKVNPGSVILSGEMMFRYMGWNEVADLLIKGLETSIAKKTVTYDFARLM
                     DGAKEIKCSEFGDAIIANM"
     gene            complement(39514..40647)
                     /locus_tag="PK937_01400"
     CDS             complement(39514..40647)
                     /locus_tag="PK937_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNH31261.1"
                     /translation="MSCFFATTICGQNLYENLQVSSGDTPVDVSRRTKGRRFTPIPLV
                     NYTSDDGAGYGLRLCVYDYDSVTVPYCRALKAQYFITTEGKWAHVLSLDYPNFLKGHR
                     FDFELAYDKLEFANYYGELSNRMVDSLHLSKSQRTFRQATPSASLTWIYDLNGPWRLR
                     GGVSIRHTSVKPNADSGNILKELKPVGYKGGVLWKLDAAMRYDTRDNYINSTCGMLEE
                     LLVEYKIGGAGDYQGWLASYEHRHFIPITPNIVAGYRLAADQVFGQIPFYDELKLGGE
                     GSVRGMPSARRRGQGRFLVNTELRWHGIRLSDRRNMMLGVILFTDAGQIYKRKDGPSM
                     TLKNWHAGYGGGLRFYWHSTIIRADYGISEGGSGLYILFSQVF"
     gene            complement(40718..41905)
                     /locus_tag="PK937_01405"
     CDS             complement(40718..41905)
                     /locus_tag="PK937_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31262.1"
                     /translation="MKTFRRVIVWFAVICCVTVVSLLFEKPRDAALAFLFIIDMATPE
                     ASELYQVVTDDIVVTHDSLDRESKKTLYYSIYSPPGNHKKSAIIMAHGFTEKGNNDRR
                     LQRMAHRMARAGFIVMTPEFPMMRRFRISMEDADNTMEAFRIVKQRPDVDSSSIGLMG
                     FSYGAGPVIMALSDSSIRDQVRFGVIFGGYFDIRKTLKYTLTGYYTTDTGQEMRSVSK
                     NNDRWKFLLGNIPESGNEKSLVIFKSIIAQKITDAGYDPGKNFLSLDSTHQSLYRFIE
                     NQNPSAFDVLYAALPNDFRSKLELADLTSYAHLIHSYMIIGHSDQDRVVPYTESLALA
                     KNLTSAPAPFVAVTNLFTHVNLKIEWGSLTVIFTETIPGFFRLWKMTYHIMRMRHIPV
                     AIA"
     gene            complement(42015..44246)
                     /locus_tag="PK937_01410"
     CDS             complement(42015..44246)
                     /locus_tag="PK937_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNH31263.1"
                     /translation="MKTFILSMFLFLQWPLVSQDSTMLSLERIFDSDELQPRFFGPAR
                     WLKNGKGYTTLEVSDRVEGKDIIKYDAATGTRTVLIGAEKLIPQGQTRPLKISDYFWS
                     SDDSRLLIFTNTRKVWRYNTRGDYWVLDIGAGKLHKLGGADAKTSTLMFAKFSPDGQQ
                     VAYVRENNIYTENLLTGKITQLTFDGSDVIINGTFDWVYEEEFGCRDGFRWSPDSRAV
                     AYWQLNADGIGVFYMINNTDSVYSKVIPVQYPKAGYPNSSCRVGVVSASGGTTVWMQT
                     PGDPRDNYIPRMEWAGTSEAIVMRHLNRLQNTLQIMLGDVKSGIVQTIATDKDSAWID
                     IDDEFKVLDGGKGFLWISERDGWRHVYKTSRDGKKTELLTPGNFDVIKVILAQEKENM
                     LYYTASPENATQRFLYRMKMDGKGKAERITTETGGWHDYQISPDAKYAIHKYSSMQLP
                     QTVEVVDLPKHKTLRALEDNLALRQRLAALKINVPEFFKIEAGNELMLDGWMLKPASF
                     DATKKYPLFVFVYGEPAEQTVVDHWGDTDYLWHQMLAQQGYIVISLDPRGTPAPRGRA
                     WRKAIYKQLGWLAPMDLAEGVRRVTTWPYIDKSRVGIWGWSGGGASTLQSMFRFPDVF
                     HIGMCVSPVTDQRNYDNIYTERYMGFPKDNLEWFLKASPISYVQNLKGKLLLVHGTGD
                     DNVHYQNTEMLINALIAAGKPFDMMAYPNRTHDIDEGEGTTMHLYSLLTRYLKEHLKP
                     GPQ"
     gene            complement(44292..44750)
                     /locus_tag="PK937_01415"
     CDS             complement(44292..44750)
                     /locus_tag="PK937_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH31264.1"
                     /translation="MRDYVLFAVLAGLAWGLGGYFEKSGLRAMGIPPIAGITLRTGVA
                     FILLALLSIPAWKQIANVNDTTAWVMIIIGGGVVAGSLGMWSFYKSLSTTENLGVTLA
                     LAFSISPIAGTVMGLFKGNQPMNWQTGIGLVTIIIGIIILQTSHAPTAKP"
     gene            complement(44932..47334)
                     /locus_tag="PK937_01420"
     CDS             complement(44932..47334)
                     /locus_tag="PK937_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNH31265.1"
                     /translation="MIKPNPKKNQFSIIGKPNPKIEGLEKANGSALYTDDLMLPNMLI
                     GKILRSPHPHALIKKIDTSKAEALPGVKAVMIGTELTEKYGVLPASQDETVLAVDKVC
                     YIGDGVAAVAALDEETAEKACELIEVEYEVLKPCLTIEDGFREDLPKIHPHTKQANVM
                     KEVHQEFGDVEEGFKEADLVMENEYFFEGNTHLPLEPHCALAQYGSDGKLTIWSSTQT
                     PHYLHVALAKVLQMKRSHIRVIKPHIGAGYGGKSEPFALEFCAAWLSKKTGRPVKFLY
                     TREEVFYAHRGRQPMKMILKTGVKKDGSITAIHMRTFLDGGSFGSYGLVTTYYSGVFL
                     TLPYKVPRYKFDAYRIYTNKPAGGPKRGHGAVQPRFAMECQLDKIAEKLGLDPVEMRL
                     KNSIEPNTMTINSFRVTSCGIKECLTSTRSRSEFDKKYASYKKQNSKVIRKGIGIAAS
                     TYISGAGKSIIWNDMPHSGVQIKVDRGGGVTVFCGASDIGQGSDSMLAYITAEVLGVE
                     ISDVHVCQTDTHLTPVDLGSYSSRVTFMAGNACIQAAERIKEMIFEIASRKLGVPVTD
                     LDNGKRLIFSKSDPNKFMDFVEAANMAESAYGTIGATGSYKPPKIGGSYKGAGAGPSP
                     SYTFSTHIAEVSVDVETGAVTVEKIWSAHDCGRALNPVIVEGQIEGSVYMGVGEALFE
                     EHIFHKNGLHRNPSILEYKVPTILDTPEIESIIVESIDPEGPFGAKEAGEGPLHAAIP
                     AIANAIYNAIGVRLDTTPFTPDKILKALEEKELAEKGGNGHSKKLRDIGAKKLVSHIA
                     TT"
     gene            complement(47394..47876)
                     /locus_tag="PK937_01425"
     CDS             complement(47394..47876)
                     /locus_tag="PK937_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNH31266.1"
                     /translation="MKSLITLKVNGDLYEIAVKSNRTLLEALRYDCHLTGSKQGCDMG
                     DCGACTVLIDNVPIQACITLAVECVGKEIITVEGIAKGTEAHPLQTAWNNQGASQCGY
                     CTPGFMVVAKWLLEKNPNPSDEEIKEALSGNICRCTGYTKIITAMKEAAAIMQKEKAV
                     "
     gene            complement(47951..48676)
                     /locus_tag="PK937_01430"
     CDS             complement(47951..48676)
                     /locus_tag="PK937_01430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH31267.1"
                     /translation="MYSKDYFESDFRCGHEDASAFNKKTDPVLPDWTEQVHLQPESSV
                     LEIGCATGYTLKAFHDAGHHVKGIEISEDAARHARDVYRLDVDTMAVHQAQFSSEKFD
                     LIYLLDVFEHLTDPVLLLKQAYQWLKPGGTLVIVIPTQTNTLFSRFGMTLFSLAGKRT
                     RIHMPPYHLFEYRPSSVRRLLLNHGFSKIDMKPVIMKPGEIGLRSSVVQNTAKYVFQC
                     LNYPLTKLSGRFGDRLTIIAVKS"
     gene            48979..50052
                     /locus_tag="PK937_01435"
     CDS             48979..50052
                     /locus_tag="PK937_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH31268.1"
                     /translation="MIWTIIYTLIFGQGIFLAIVLFFNKKGSVLANKSLALLMLIFSS
                     AVLEQLIRISGLFSALPHMLFISVPFWYALAPAYYLYAKNLVEKQNQFVIKDAWHALP
                     FLGVILLLSPYYLWDVQKKIAYIIDPSQFNDFALESIILNVVMVLQNVMYLTMAIRLL
                     SQNEAYSKLNRIIRFLFSIMMGYVVFNFINAIYFLFTGETLVHWGNWSIPIFATIIYT
                     IAFLSFVSPETIFFTLWPRNNNSLSKERKHEVLNQLYKLMEQDKIYREPDLRYTEVAA
                     RLGISARCLSALLSEDIGMTFNVFVNTYRVKDAQNQLRERYCEETLLAIALDSGFSGK
                     SSFNRIFKEQTGLTPSEFVYQSN"
     gene            complement(50128..50784)
                     /locus_tag="PK937_01440"
     CDS             complement(50128..50784)
                     /locus_tag="PK937_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH31269.1"
                     /translation="MKRFKKIIAVLLSAAFWNIQAALLFSHSIHAQQGTRPLVAVLDL
                     QVTGGILKSEAQSLSDRLRGEMQGLKKFDIIERSQMDALLKEQDFAMSDMTEANAAKA
                     GKLLSVEQIVVGSIGRVGRSYTVDVRLIDVVSGRVITASQQDYNGPTEGLTQVIRNVA
                     RQFAGLPPLKVSVASNTKWWVMGSLLAVGGGVAAYMMLNKKESGSSGPPAFSDPPALP
                     "
     gene            complement(50805..55256)
                     /locus_tag="PK937_01445"
     CDS             complement(50805..55256)
                     /locus_tag="PK937_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH31270.1"
                     /translation="MARWIRLLLMCCIVPIQAQSDWINLGPYGGEYETMAIDQKKPNN
                     LYIGTRNGQIYRSTNSGQTWSFLSAIDGTITKLVVDSSNGNNIFVGVHDRNNFNHVFY
                     SKDAGLTWTGNYALNLYNEEIKDFVINPANNTQMHLVTVNRSIPSINGGKSFVGSGPY
                     FPDGFHSFAYDPHDPTNVMYAAGHDNSFDRDIFQGTRNAWSNVHTYQYMETGWRIGKV
                     HVENTNIAAVYVLIGNEDSTKIMRSLDYGATWAIVFSDDVAMYQGGFKIGQYAWLHRS
                     KGNNEHIVATQNGILKTNNGGLSWYRMKDGLNNNLSLVRGGTVVRSPQDTSIFYCNYS
                     KKPARLSITPYGYVANVNQSLGFTAQEIKKVHYSQNNYVWAETQTRDLIYTSNYGQDF
                     YETGIMLPPGDEIIGGNGFFVTKRGSYLQSAGAVLGAITFPSGTDSVIAVIGSEVFSS
                     GLYDNYEFGITQNSTGGTSSGFFIARYDFSTNNVISYIPTLPDIALTSPITSFSFSFR
                     SYQRDTVDIYVAASTGIATTYFANGTFGSWKTIKLFDGSFTIKKIEFRETEAFIWAKE
                     PGGDRLFIGHRDDMSSDFSIEEISGSFPRDEFGQSEIHDIHAGVDGYYMIRFRSDFGA
                     NGAEYTRPLYKAGFGKEPRWTEITTPDLSKFNIQNVLTEYRFQENAVVIYAAANNGIW
                     TMTQTASISIDTLVIFSSTSALLDSSIAELKYANTGTAFSYIERFEITDDAENSFSIA
                     DKNFADGDILFLDIRSETSFPIKFKPVSKGLKTAKLKSYYTVRASNGSDSTIIATTRL
                     VGIGKTSQIGTNLKNDTLDVKGALVNASKTTNFQLFNSGEDDMYIEDIYLSKNDVFAI
                     QSINESDSTSFIVAPGRVLPVILKFEPTEGKSYTDTLIIESTAFKANNTPNPKHRIVV
                     KGVGSSIQVSNIDNLSASLNQALTIEVSLSQIDALEVTGLLRYRKNGEGSSGYKNKTL
                     TPTVTQSGNSSRFTTTIPQEDITQNGLEFYVEFTSSSSVSPIQYPENGEFEPQVVSVT
                     IPNPGLSSSGYVSLPGGTDKKAFQLMSFPLNLTNKTVGGAFTPSNMGEAGVKGDWQVY
                     RYDNDLSKFISSSDPDITFGNIESGKSYLLVSRKPKTINSGAGKTVKKEDAHVTIQPG
                     WNMIANPYAFSINWSDVANFNDNVELTNIVVLQNGRFKYVDEVDVYNLKLEPWKGYMY
                     YSPEENGTFTINYPAINSDNASALQKKEIRTTVDKSGYRFLLTLHDDGEDIVFEAGTA
                     EDAQDGADGRDKRALPMLMKDEVSLHWLQADRKLQTDMRAVSPEGHVYTFEVATGVDR
                     RTVSLSYSATNLPEGFSVRMVEVTTGMVQTGETLNLMVQNGKPKMYKLLIGTEAFIAK
                     AQSEIIPSQFSLAQNYPNPFNPSTTLRFGLPEASSVKITIYNMLGQQVRTLVKQVMPA
                     ASHALLWDGTNDFGQRVASGVYVYRLEATGLGSNKVFSQTRKMTLIK"
     gene            complement(55454..56131)
                     /locus_tag="PK937_01450"
     CDS             complement(55454..56131)
                     /locus_tag="PK937_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNH31271.1"
                     /translation="MKSFMIKMTALLACVATVITATLYLAEANDGKAVAIVVKSTGKA
                     QAKQGNKWVDVTKGSRLFSGDKVVTKDDGFAAVMFLDDKSIIKLKPNSNLEVKGQYEG
                     KSISKNIVMDVGELFVKVSKQKGSFQVATPTSVASVKGTEFWVIENTNGTKVIGIEGL
                     IEVLNKASNKKQDVSAGNTANSDKSGNLNVDTTKENEIPEDKDLEEIIIKFRDNDGKE
                     KEVKIKY"
     gene            complement(56175..57308)
                     /locus_tag="PK937_01455"
     CDS             complement(56175..57308)
                     /locus_tag="PK937_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31272.1"
                     /translation="MELKAVLSRFRQMVGLSLIVAVFSFTTVVAQGTTNGVTITPEPL
                     TPFTFTSFPLSLLGIGGSDANLSSFNNFGLRFENNSGSVKIIRMGIDLKTSQGSIFNK
                     KYLVTLTIPSGVPIFVGVPQILNGSFGSIKASNRQSISISDDFMKQIKGGFPSGLFTF
                     DIKAGLPNVNNYDGLDYMGSLQIQVLGNSPVEIISPSFGFESIDGLPQFAWSAQGATK
                     FLLTIARKKGSQSDEGAIKSVVQRAVIELDNVFSYSLPSGGPSTSKENNLNCNPGLEP
                     GDYVFQVTAVIQDNFTGKSRNVDSPIRAFTVKETISSTAGLNTEEILGLLNDAAKGAV
                     NFKDQLKGFKAVSISIKGNTATIDDLRTAVQNLPEAVKFEIKP"
     gene            complement(58032..58742)
                     /locus_tag="PK937_01460"
     CDS             complement(58032..58742)
                     /locus_tag="PK937_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH31273.1"
                     /translation="MNKKFELPLCDQCHSRVHSVFGDLKDSDVKSLNENKCGNIYKKG
                     QVLFYEGNKPTGLFCVNKGKIKTYRINADGKEQIIRLAKEGDVLGYSSLISGDLYSSS
                     AAVIEDAIICYIPRNVFLNLLDSNTTFSMKMIRLLSHDLKSSEDRVVNLAQNTVRERL
                     AETLLMLEEFGGTENDGKTLRTSLSREEIANIVGTATETTIRLLSEFRSDGLIELEGK
                     RIKIVDRGLLLKAANVED"
     gene            complement(58769..59287)
                     /locus_tag="PK937_01465"
     CDS             complement(58769..59287)
                     /locus_tag="PK937_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNH31274.1"
                     /translation="MKYKVTTEQKEYDIEVVPQGASFKVRVGKSPDIEELDVDFHTVG
                     QGSVHSMLVNDKSYRVIIDKKMHKHAVYTRRHRFEFNVEDERTFLMRSLIAGSGAKAV
                     GEVKAPIPGLVSKLLLQEGDTVNQGQGVLILEAMKMENEIKAPMAGKIKKFHVQGGKN
                     VEKGQPLFVVEP"
     gene            complement(59358..60119)
                     /locus_tag="PK937_01470"
     CDS             complement(59358..60119)
                     /locus_tag="PK937_01470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31275.1"
                     /translation="MRQKYYLVSGAGTGIGLAIAKKLAERADHSVILCGRRQDVLSEA
                     MHQLSNTRNHSILVMDIHNPGTIKKAAAQLAIPYLDGIISNAGIGGENQYGDEDRWDA
                     IIATNLTGTYHFVNNFLPIVRKSPNTYRHIVVVSSILARLGVARYSAYCASKAGLLGL
                     MRSWAVELAPEKILVNAVCPGWVNTKMAQDGMEGIAKNIGVTTEEFYRMAMESVPLRK
                     MSEPEEIADLTHYLLKQRSITGQTLDINNGAIMNS"
     gene            complement(60132..60743)
                     /locus_tag="PK937_01475"
     CDS             complement(60132..60743)
                     /locus_tag="PK937_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH31276.1"
                     /translation="MGSGIKIKVTTLAIKNMVCDRCIRVVREELVNIGVNVRHVELGK
                     AIVEGNLTLPMQNKLRTVLTQNGFELLEDKRAKIVEEIKNQIVIYVQKNDVENKNIKL
                     SVLLSRTIGRDYSYLSGLFSSVEGITIERFLILQKIEKVKEWLAYDDRTLSEISYKLG
                     YSSVPHLSKQFKQITGLTPTEFKQQNDHRRIPLDKIKIIHRIT"
     gene            complement(60728..61951)
                     /locus_tag="PK937_01480"
     CDS             complement(60728..61951)
                     /locus_tag="PK937_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31277.1"
                     /translation="MLVNAKIRTIIQWEFTQRVISKGFLFSVIFIPAIILAFTLLPVF
                     FANSNGNRIFSVGVFDATRRLGEDLSSKLQDLTYSEIQLVPLASSNVEEALAEGKRLA
                     KEDGIDAVLVLTADMFTQRKIEIYTDYRTENAVQIELLKQAVAGLLMEYRMRGKNFTH
                     EEIQNITAPLQVDTYKLGSDHAASLQKYMTGIILVMMLYFAIANSAGSFMRGLSEEKN
                     NRVIEVLVASASPREIMAGKVIGLGLVGLLQIAVWTTLAVMLGSRKEIAALSPELLLF
                     FILYFVMGYMFFAGINALIGAIFASDQDIQPLQAVISMLGVVPVALAFWVIEVPSDIA
                     IRILSYVPFLTPTMMILRIIVSNPPWWEIGLTFITLFVGVVLVFRMAGRLFEISLRMH
                     GKKISFREMVRWARA"
     gene            62449..63519
                     /locus_tag="PK937_01485"
     CDS             62449..63519
                     /locus_tag="PK937_01485"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH31278.1"
                     /translation="MAKRVSWFFLLAIVTMVLCSCRTLYHTETIVFYNNQIRFSGTFY
                     KPLTSAPYPVIVIAHGSGTADRNSFFYKPYGEYFSKHGIAVFIFDKRGVGDSEGVYDD
                     SPDFQLLASDLKAAFQSLSSRNDIDKNKIGIVGISQAAWVLPITAQNLDGLSFVVCTS
                     CPSVPPYESDLFQKGRELIEAGYSQNDIDDILNYNRAVTEYVATFVGREKVLQMKHRF
                     KDRQWFRDFNYNPDLSPEDTLKQPQYDHYRNSTFDPMPFWNQVKTPVLFVYGSKDSHI
                     PVVQSIKRLEDSIEADQVNYRIKTYRNAGHLIQSVEDSVESLKFSLFRFIKGRPQPIP
                     EYLEFVRRWVLAPSQVDADTVL"
     gene            complement(63612..64175)
                     /gene="frr"
                     /locus_tag="PK937_01490"
     CDS             complement(63612..64175)
                     /gene="frr"
                     /locus_tag="PK937_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNH31279.1"
                     /translation="MYDLNKQYHETEEKMKKSLETVRNELAKIRSGRATTTLLDGVKV
                     DYYGTPTPLNQVGNVSAPEARLLTVQPWEKGMVPAIEKAIREANLGLNPQNDGALIRI
                     PIPPLNEERRKELVKLGKKYAEEGKVAVRNVRRDANEHFKKAEKDHKISEDDSKDAQD
                     KVQKMTDNYIKKIDEMLVIKEKEIMEV"
     gene            complement(64214..64930)
                     /gene="pyrH"
                     /locus_tag="PK937_01495"
     CDS             complement(64214..64930)
                     /gene="pyrH"
                     /locus_tag="PK937_01495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNH31280.1"
                     /translation="MPIFKRILLKLSGEALMGEQGYGIDTKVLEQIAKEIKDVKALGV
                     EIAIVIGGGNIFRGLAASAKGMDRVSADHMGMLATVMNSLALQDALEKQGLFTRILSA
                     IKMEQVCEPFIRRRAIRHLEKGRIVIFAAGTGNPYFTTDTAASLRAIEVEADVILKGT
                     RVDGVYDSDPEKNKEAFRFEKITYLDVVKKGLNVMDMTAVTLCMDNKLPIVVFNLKTQ
                     GNLRRLVLGENVGTRVQQEQ"
     gene            complement(65054..65950)
                     /gene="tsf"
                     /locus_tag="PK937_01500"
     CDS             complement(65054..65950)
                     /gene="tsf"
                     /locus_tag="PK937_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNH31281.1"
                     /translation="MSAVEISASAVMKLREETGAGMMECKKALTEAGGDAAKAVEILR
                     ERGLKKSAEKQSRVANEGTVLSSVNDAHNFAVMVEINCETDFVAKNDDFVKFSNNVLA
                     QVRDSKPANLDAFLSAPAAFAAGQTIADAVTGLTAKIGEKIAVKRIAVIENNQGVIAG
                     YIHPGNKLGVLLQLNVEGFAATMTADAYSVAYDIAMQTAAMNPTYIHREEVPADVVEK
                     ESAILREQLKNDPKNANKPDNVLTNIIKGRLDKFYQEVCLVDQAFVKDPNKMIKDLVA
                     DLSKKLGKPVTIRRFERFRLGE"
     gene            complement(66001..66843)
                     /gene="rpsB"
                     /locus_tag="PK937_01505"
     CDS             complement(66001..66843)
                     /gene="rpsB"
                     /locus_tag="PK937_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNH31282.1"
                     /translation="MSEVQVSFDPKKKTEELLAAGVHFGHLTRRWNPKMKPYIFMEKN
                     GIHILDLRKTVHSLEGACKALAKIVANGERVLFVGTKKQAKEIVQNEAKRANSFFVTE
                     RWLGGMLTNFSTIRKSIKHMKNIEKKEIDGTFEKITKKERLMLEREKAKLKIVLGGIE
                     DMKQLPGAVFVVDVKKEHIAVKEANVLGIPVFAIVDTNCDPDTVQHVIPGNDDATKSI
                     EAITKTIADAVAEANQHAVAAAKEKEIAEVDEERVKDETRMKYEETNFNKKRLGKKEV
                     KKED"
     gene            complement(67050..67439)
                     /gene="rpsI"
                     /locus_tag="PK937_01510"
     CDS             complement(67050..67439)
                     /gene="rpsI"
                     /locus_tag="PK937_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNH31283.1"
                     /translation="MSAPLAAVGRRKTATARVRLVPGNGKVTINHRDIKDYFPRGSHQ
                     IHVTEPLTVVEAGSKYDVIALTRGGGPSGQAGAIRLGIARALVKIDEEMKKALKPIGA
                     MSRDPRMKERRKYGLAKARKRYQFSKR"
     gene            complement(67459..67875)
                     /gene="rplM"
                     /locus_tag="PK937_01515"
     CDS             complement(67459..67875)
                     /gene="rplM"
                     /locus_tag="PK937_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNH31284.1"
                     /translation="MPKADQIKQKWFVVDAADQTLGRFASRVASILKGKNKAYYAPHM
                     DCGDFVVVVNADKVRLTGKKTKIKEYIHNTMYPGGQRITKFEDMIRTKPERVVYLAVH
                     GMLPKNTLGAKMIKKLKIYAGEKHPHSAQNPETLKF"
     gene            complement(68120..69220)
                     /gene="thiH"
                     /locus_tag="PK937_01520"
     CDS             complement(68120..69220)
                     /gene="thiH"
                     /locus_tag="PK937_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNH31285.1"
                     /translation="MPNINLNMSFYTRWQSYRWPVVENLLGTRTKKSVENILADASDF
                     KFPEDVITLLSPASDTYRHVIAQQAAQQIQRPIHLYTPLYLSNYCHNSCTYCGFRVEN
                     KIQRVVLDQTQIEKELQAIAATGVRRVLLVTGEERRFAGVTYIAEAVRIAKTLFDHVR
                     IEVQPLNTDDYALLREAGCEGVYIYQETYHETNYRLYHPRGMKSHFRHRLETPDRIAM
                     SGISKIGLGVLLGLENWRVDAFFLAMHAMYLKEAYPKLQIAISFPRIRPAVGVHNKVS
                     DVFESDLHHLVAVFRWINPDWDIAMSTRESPDFRNRTLKLGVTSISVGSKTQPGGYLK
                     NDTAELEQFAVHDNRSAETMRSFLEKNVRMYD"
     gene            complement(69207..69923)
                     /locus_tag="PK937_01525"
     CDS             complement(69207..69923)
                     /locus_tag="PK937_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNH31286.1"
                     /translation="MFEIYSALLVGVLGSFHCIGMCGPLALAIPQHDRRWFMLVRSAL
                     LYNGGRAFTYAMMGLVVGAIGATLQTVGLQQGLSIGLGVAILLALLLPNSIKQKIWPD
                     SSLQGGWAAMRRWFGQLLRDPNPTSLFTLGLLNGFLPCGLVYIALGGAMATGHAWEGA
                     LYMALFGLGTLPAMTAVAIFGQFITTTTRQKITKLIPVVVGGVAVLLILRGLSLGIPY
                     ISPDMHAVAAGSGESCCAKH"
     gene            complement(69942..70388)
                     /locus_tag="PK937_01530"
     CDS             complement(69942..70388)
                     /locus_tag="PK937_01530"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNH31287.1"
                     /translation="MSLFRNWGFRITVLYVSFALLILGMVFVSSISREDLVSTNYYDN
                     EIAYEKRIQAIKRTKMLEQKPAWNISGRVLTMIFPEQISQITGGTLTLYRASDADRDI
                     VVPLKLDSQRSQAIDLQKAIPGMWRAKLEWKSENNDYYLEDVIYIE"
     gene            complement(70400..71830)
                     /gene="ccoG"
                     /locus_tag="PK937_01535"
     CDS             complement(70400..71830)
                     /gene="ccoG"
                     /locus_tag="PK937_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNH31288.1"
                     /translation="MAEVKEELAQIKNADGETYRDRIGIIDKKGARIWIYPKLVAGVF
                     HYWRVIVSIILLFVLFGVPFIKVDEHPFIMFNILERKFILFGLAFWPQDFYLFALTFL
                     SIVIFIVLFTSVFGRIWCGWACPQTIFMEMVFRKIEFWIEGDGPKQYALDQAPWNADK
                     ILKKTFKHVIFFAISWIIGNTLLAYIIGVDELFKIITDPIGMHVNGFTAMMIFSLIFY
                     GIFSRFREQACIYVCPYGRLQSVLLDKNSIVVMYDFQRGEPRGKLKKGNTGGESLGDC
                     VDCKLCVQVCPTGIDIRNGTQMECVNCTACMDACDSVMEKIKRPKNLIRYGSYNLISQ
                     KLHFKITPRIVLYSAVLTVLITVTSIFLFKRKDIQTTILRAKGPIFQLMENGDIRNVY
                     TAKVLNKTFDAMQIEFKLEGVEGNIALIGTHELRLHPDELVESTFLVDIPRNSIHTMN
                     TKIKIGVYKDGMKVSTEETSFVSPMQ"
     gene            complement(71855..72520)
                     /locus_tag="PK937_01540"
     CDS             complement(71855..72520)
                     /locus_tag="PK937_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNH31289.1"
                     /translation="MAEVKDELLDHDYDGIREYDNPLPPWWVNMFYITIVWATIYLIF
                     YHVLEIGDRSVDEYRKEFDNTWTKEMDPNYQKVDMLGTGYKGPYYYKPSDPTNKGVVA
                     FMPAAEKPKEEELTKEFAALTDAVSLGKGKEIFTVNCVPCHGKLGEGGIGPNLTDDYW
                     IHGAGINNVVRTAWKGVPEKGMISWKQLGEKNVLLVASYILTLRGTNPPNAKAPQGDL
                     VKQ"
     gene            complement(72522..72725)
                     /locus_tag="PK937_01545"
     CDS             complement(72522..72725)
                     /locus_tag="PK937_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNH31290.1"
                     /translation="MLRRILENAGGLEIWPTIALVIFFVFFVGLIAWLVRIDKRHLHH
                     METMPLDGEEIAVSNNGYKHGGK"
     gene            complement(72726..>73467)
                     /gene="ccoO"
                     /locus_tag="PK937_01550"
     CDS             complement(72726..>73467)
                     /gene="ccoO"
                     /locus_tag="PK937_01550"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNH31291.1"
                     /translation="CDTYSFANEKAEAPALTKEYVSHGQSYYHRLIERKPLPFIILTL
                     IAVAIGGVLEIVPTFAVESNIPTIASVKPYSPLELQGRDIYIREGCYTCHSQLVRPFR
                     SETERYGEYSKAGEYVYDHPFQWGSKRTGPDLHRVGGKYTNSWHYSHMMDPRAISPGS
                     IMPAYPWLFENSLDISTTEKKISAMQIVGVPYEKGYEKVANNDLKAQATAVAGDLTQN
                     GFPTTPDKEIVALIAYLQRLGTDIKVKQ"
BASE COUNT        20697 a        16999 c        15358 g        20413 t
ORIGIN      
        1 taaaggatat catgaatata ctagttctca acagcggcag ctcttccgta aaatttcagg
       61 tcatcaatac ggacctcacg cagatccgcg acaatgcaga tacacgcctt gcctcgggta
      121 tcatcgaacg tatcggcagc gaatccatca tttcgtttaa agccgaaggc caacctacgc
      181 tcaaacaaac cgcaccgatg cgcgatcata aaatggccgt cgataaaatc gtcaaatgga
      241 ttatcgggaa agatacaaaa ataaacggca tcaacggttt atccgatatc cacgccgtag
      301 gtcatcgcgt ggtgcatggc ggtgagaaat ttacccgctc gattttactc aacgagcaaa
      361 tcatccaaga gatcgaagat tgtattgaac tggcgcccct gcacaatccg gctaacctca
      421 aaggcattta tgcggttacc gaaattttcg gcgcaggcat cccgcaagtg gccgtatttg
      481 atacggcatt tcaccacacg atccccgaag aagcttatct ctatgctatt ccgtatcagt
      541 tttaccgccg ctacaaagtg cgccggtatg gctttcacgg tacgtcccac cgttatgtcg
      601 cgtatcgtta caggacattg acggggcaag ccaaagagca ggtccacatc atgacactgc
      661 atttgggtaa cggcgcctcc atttgcgcca tccgtggcgg aaaatccgtg gacacatcca
      721 tgggtctgac accgctggaa ggacttgtta tgggaacgcg ttcgggtgat attgatccgg
      781 ctgtattgga gttcctctcc cataaagaag gatattcgct gcaggaaatc gaatccgtac
      841 tcaataaaca atccggtttg ctcgggatct caggtcttac caacgatatg cgtgacatca
      901 tcgcggaagc gaaagaaaat gatgatcgcc gcgctaaact tgccatcgaa atatattgtt
      961 atcgcattcg caaatatatc ggagcatact gtgccgccgt cggaaaaccc gaagctttag
     1021 tatttgccgg cggcatcggt gaaaattcgg cgtatattcg cgaaagtatt tgcaaaggca
     1081 tggagttttt tggattaacg cttgatcccc atcttaatgc cgcaaccgtc ggcggtaaag
     1141 aaggcgtcat ctcttcgccg gatgcacgcc tcaaaacatg ggtcatcccc accaacgagg
     1201 aactgctcat cgcgcgagat acggtgcgtt gcgtagaaaa agcgccgctg ccgtgatggt
     1261 tgagtgactt tatcaacgaa ataacgcgat tcgataatct gaatcacaaa aaaaattgac
     1321 actacgtttg tattcattgt ttttttcaaa ttgaatagca ggcattagaa ccctggctca
     1381 aataatctga cacctcttcc cgcaaagacg gagcttgatt ttagggcaat aaataaggaa
     1441 attcgcccgt actaatatga attatatctt acgggaagct tgggatgtat taaaaaatcc
     1501 cggcattatt cccaaactgc ccaacatgat gcgggcatcc gcatcgtacg cccttaataa
     1561 atggacacga aaaaatatcg tgtggggtta tccttttgtc atgatggtgg agcctaccaa
     1621 tatctgcaac ctccgttgcc cgctatgcat cacggggagc aaactgatga cccgcgacga
     1681 tggcatgatg acactggaac gatttaaaca attgatggac gaaatgggcc cctatctgat
     1741 gcatctcaca ctatggagcc aaggcgaacc tttcgtcaat cgtgattttg taaaaatgat
     1801 ccgttatgcg catgacaaac atatcaaaac ggttaccagc accaacggtc atttcctcag
     1861 tgaaaacgcc gaagcgatcg tcgaatccgg tctggacacg ctgatcattg ccgtggacgg
     1921 cgcatcacag gatacgtacg aaaaataccg cgtgaacgga aaatttgaaa aagtattcaa
     1981 cggtatgaaa gccattgctg ccgcgaagac caaattaaaa cgaaaaactc cggcgatcga
     2041 acttcaattc atcgtcatga aacataacga acatgaactg gaaaccattc gtcaaatggc
     2101 gcacgaatgc catgtacagg tattatcgct caaaaccgcg caagtctata ctgcggaaca
     2161 agctctggag tttttaccaa acaacgaaaa ataccggcgt tatgaaatcg gtaacgacgg
     2221 aacgttcgcc gcaaaaatcg ggtccatcaa tttttgccga tgggtattga tgtgccctgt
     2281 gatcaattgg gacggcaccg tatcgccatg ctgttttgat aaaaacgcgg attacccgtt
     2341 gggcaatgta tttgatcccg gcggaatgaa aaccatttgg aaaaataaag cgtaccatga
     2401 tttccgtcgc cgcattttta cacagcgcag cgctataccg atttgcagca actgttccga
     2461 aggactcgaa gtcgaagttt atgaaaaaga attgatcccg tcacgaccgg catctgcggt
     2521 agcgctggat cacgtcgctt aaccataaaa ggccgccatg agcaagaaaa aggaaaaaaa
     2581 gaaatccgaa aaaaagcagg gcaaaaagaa accggtcaaa ccggtttccg taaaaaaatc
     2641 aaccggcaaa aaagccggtt ctaaacctgc atccaaaaaa gcaaaaccga ccaaaaaaag
     2701 tgtcgcggcc aaatccccgt ctaaaaatac agccccctct gttgataaaa aacaggcgtc
     2761 cgctaagtca accaaaaaac aaccgtcccg ccctccgatc ccgcgtacac cggcagataa
     2821 cgtgcggcga ccttttaaca taattgagag ttatatgggt gaatatcgtt ttaacgacat
     2881 gtttgcaata ctttttgttt gtaccggtaa tatgtgccgc agtcctttag ccgaaggtat
     2941 ctttaaacgc aaattggcca ttgagtgtcc cgaatccatc aaacacaaaa tctgggtaga
     3001 atcgtgcggc atatacgcgt acgacgggaa taagccttcg gaaaacgcca tcaaagtcgg
     3061 ccatcaaaac ggcgttgata tttctgcaat tcgatccaaa ccgatcaacc gcgtgctcgt
     3121 cgagcaatcc gatcttattt ttgcgttgtc cattgatcat ttgaattata ttcaggaaaa
     3181 tttcccgacc gcgcgtcata aaacatattt gatgaaagta ttcggcaaag atcgcggcgt
     3241 cacgatttcc gattcgattc ccgatccgat gggttttagt atggactttt acctgaagac
     3301 ctataatgaa atccatgtta ccatagatga gatttttcaa aaagtcgtcg ctatggccga
     3361 acaaaagctg aaccccaaat catccgaaag catgtaatgc gttttgccat cgtttttttg
     3421 ttcttttata taagcggtat cgcacatgtc acggccggcg accccctgcg aacgttgccc
     3481 aataaggcgt ttcccgtagg ggaaaaactc aaattttcca tcggctggga attcatcagc
     3541 gcgggaaccg cgacattgga ggtagagcaa ctcaccgcac accgcgatcg cccgtgttat
     3601 cttgtttccg cgacgaccgt atccaatgct tttttttcta ccctgtatcc ggtcaaagat
     3661 cgtttggagt cgtacattga tcagaacgga ttgtatcccc tgaaatatgt gaaaaatact
     3721 tccgaaggcg gctataaacg taattttact accgagtttt atcacgaaca acgcaaagcc
     3781 acgatagatg atcgcgactc cggatacaaa gaaattgcaa ttcctgaaat ggtacaggat
     3841 atcatttctg cattttatta tttgcgcaat caatcgctgg aaatcggaaa aacgctatcg
     3901 ctcgcgacgt acgacaacgg caaaataaat caggccacgg tgaaggtggt tcgtaaagaa
     3961 accatttccg tagatgccgg tgaatttcat tgtattcttg tacaaacgcc gatcgggcct
     4021 tttaataata aatccgatct gaatatttgg cttaccgacg atgatcgcaa aataccggtt
     4081 ttgatgaaat ccaaaattgc catcggatcg gtacgggctg aactcgtcga aatgagcggt
     4141 actttttaag ttcacatcaa acctgtcatt ggtacgctaa cgggagaaaa ccatgcagca
     4201 ttacgttcac ggatggtatt ccgaacgcct cggcaaacaa atgaactttc agcattttgg
     4261 ccacggcggc gcgccggtcg tggtatttcc caccacactc ggcaatctgt acgaattttc
     4321 cgatcgtcgt atgatcgatc cgctcgccga aaaaatcgaa aacggactga tccaaatttt
     4381 atgcgtggat tcgatcaata acgaaagttg gtacaacgat cagattcatc cgcatgaaaa
     4441 agttcgccgc catgttttat acgaagatta tctgatccat gaatttttac cctatttgcg
     4501 taataaaacc gggacggatt acctgattct tttaggttgt agtttcggcg gttatcatgc
     4561 ggtcaatttt gcaatgcgtc acccggaaat ggtcaaccgg gccatcggct tgagcggttc
     4621 gtacacgatt caaggctttc tgggcggcta ctacgacgac ttgtgttatt acaacaatcc
     4681 cgcacattac gtgcaacatc tctccgaccc ctattatatt gaccgttata atacacacac
     4741 ggaaatcacg cttgtaacca gtgatctgga tccttgccgg gagcgcaacg aatattttca
     4801 tcagattctc tcggaaaaag gaattcgtca caattatttc ttttgggacg gaggcatcgg
     4861 tcatgattgg ccttactggc aacgcatgat cgggcattac gtttaaatcg ctttagccta
     4921 cattattatg atcggcatac gtttactact atttatatcc gttcttttct ttttttattt
     4981 tcttgcgcgc caacccgata cggccgaaac catgcgttgc gacgcgtcgt tttacggtca
     5041 cggccgggta ccggtcaaat tgcgcaggcc ggtaaacgat tcgatccctg caaccccgat
     5101 tcgcgtagca cagtttttca ggtggacgct atcgcaagat gcgttgatcc aacatatgga
     5161 cgtcttacct gaatcggaac ccgttacagg atttgaagac cgtgccgtct gcattgaagg
     5221 ttacgccgtc gctatacgct acgatcataa agacggtctg tatcaaaaag acaatgcgct
     5281 gtatttggaa atgaccgatt caccgcgttg ggatacgccg catctgatcg tacggatacc
     5341 gccgggcgag gcttattgcg atgcgcgtac aacattgtgg aacatcgcac attgggatgc
     5401 ttccgcggcc aaagaaaaaa tttcggccgg tctgcgtttg atgcgcagac ccacacgcgt
     5461 acgtgtgacc ggttttttgt atatcaatcc ggaaaccaaa aaaaaatacg accggcacgg
     5521 catcagttat caaaccaaag gtattcctga cggcatacaa cgaaaaagaa caccgccaca
     5581 aacgcaaggc gtttgggaaa ttcagccggc ttttcgggtt gaattgataa actaactatg
     5641 cgtatgaaaa caccactcaa aattgctttg gcttcaagtg aagtcgctcc gttcgccaaa
     5701 accggcggtc tggccgacgt atcgggcgcc ttgcccaaat acctgacgcg gttgggatgc
     5761 gacgtcaaag tgtttatgcc caaatatgtt tccgtggatg acagtaaatg ggatttacat
     5821 tactgtgccg atatcggcga aatcctcatt cgtatgggcc gtgttacacg gagcgcccat
     5881 gtacttaagt ccgttattcc cggctccgaa gccgagattt attttatcga atgcccgcac
     5941 tattatcatc gccgccatat ttatacgatg gatcgcgacg aagatgagcg gtttattctt
     6001 ttttgtaaag ccgtcatcga agtgatgcag cgtatccaat gggcgccgga tgtcgtgcac
     6061 tgcaatgatt ggcagacggg gttaattccg atctacctca aagataatta caattgggat
     6121 cggatgtttg atcgtacggc gtgtttgtac tccatacaca atatcgctta tcagggacgc
     6181 ttctccaaag atacgctgta tcgcgccgaa atgcgcccgg atcagttcta tcccggcggc
     6241 ccgatggagt ttcatgacac gtttagtttt atgaaaaccg gtattctttc atccgaaatc
     6301 gttacgaccg tcagccatac gtatgccaaa gaaattctga cctacgaata cggtaacggc
     6361 atgaacgatg tgctgtggcc gcggcaaaac gatcttttcg gcgtattaaa cggcattgac
     6421 tacgatgaat ggaaccctga aaaagacaaa tacatccccc atcctttttc acagtccgat
     6481 ctttccggta aagaaaaaaa taaaaaattt cttttggagc aaacgcatct gacgtatgac
     6541 ccggccaaac cggtgatcgg catcgtttcg cgtttagtcg gacaaaaagg atttgatctg
     6601 atcgaaaaag cattgccgga gctgatgctg cttgatgcgc aatgggtcat actcggtagc
     6661 ggtgaagacc gttatgaaga tatgttcgaa aacctgcatc acgctttacc ttataaagtg
     6721 tggacctata tcggctatag taacgaactc gcgcatcgta tcgaagccgg ggcggatatt
     6781 tttctgatgc cttcgcactt tgaaccttgc gggctcaatc aaatgtacag tcagcggtac
     6841 ggtacgatac cggtggtgcg aaaaaccggc ggacttgccg atacggtttg ggattggcat
     6901 gaactgcatt ccgcaggacg cggcatcggg aacggtttta cttttacgga tgcgtcaccg
     6961 tatgctttat ttacgacctt attgcgtgca ctcgaaacct acaaagacaa aaccgtgtgg
     7021 cgaaaaatcc agcagaacgc catggcaatg gaccattcgt gggatgcatc ggcccaaagg
     7081 tatatcgaac tctataaaat ggcgattcaa aaacgccgcg ccgaatagaa gaacaaataa
     7141 ggattggata gtaagcctaa aatcggtttg tactgtagac ttatttacaa ttgcttgact
     7201 ttcattgacc tttaatctaa ttttttgact gcacttcgtt taggaacgcc tcaatccctt
     7261 caaatcagcg ttccgccttg ttcataccga ctacaggagg atattttgag actcaaaatc
     7321 aagtatttag acggcattcg cctcaaacgc tccgtgatcg ccggatgcaa tcacgttatc
     7381 aacaaaaagg aatatctcaa taacatcaat gtgtttccgg tcccggacgg cgatacgggt
     7441 accaatatgg catccacatt gaaaaacatc gccgagacgg tcgaaaaaga agaattgcat
     7501 gagattgatt cgatggcgat cgtgctggcc gattcggctc tgatgggtgc ccgcggcaat
     7561 agcggtgtga tcatggcgca gttttttcag ggcatgaatg aaggattgca aggcagcgcc
     7621 aaatgcacga ccaaaaaatt ttccgatgcc gtacaaaaag ctacaaaatc cgcatggagc
     7681 gcaatttcca atcccgttga aggcaccata ctctccgtca tacgcgactg gggaaaatcc
     7741 gtagaaaaaa atgccgaaaa aacggatgat ttttccgaac tattccgtga aagtcttgcc
     7801 gatgccaaac agtcgctcgc caatacgccc aaacaaatgg ccgctttagc gaaagccggc
     7861 gtcgtggatg cgggcgcaca aggatttgtg accatgctgg aaggcgtgct ggatttcata
     7921 gaaaacggac gcatttccga catcaagcgt caaggcctat ccgaaaccga agacgaagcg
     7981 atggaagcac acattgatga gcacgccttt gaagaaatca cgtatcagtt ttgtaccgaa
     8041 tgtttgcttg tcggcgaaaa catggatcgc gaaaaaatcc gcgaaagtct caacaaattc
     8101 ggcgactccc tgatcgtggc cggctcaacc aaaaaagtca aaatccattt gcactccaat
     8161 acaccggaag acgtatttga agtattagcc gatcatggac acgtgatttc caccaaaata
     8221 gacgacatga aacagcagtt tctcaatgcg cactccgaga atgtccaaga gacggcaatt
     8281 gccattgatt cgtgctgcga tcttccgctc gaatatatcc gccgaaatca tatccatgtc
     8341 attccactca acgtgcattt tggcgaacag atttatatag atcgccttac gttgacaccg
     8401 aagaaatttt atgaaaaatt gacgtcttca cccgatcatc ccaaatcttc gcaaccgacg
     8461 ccggctaaat ttaaagaatc atacgaacga ctcttacaaa tttacaaaaa cgttgtttcc
     8521 attcaccttt cctccaaact cagcggcacc taccaaggcg ccctcagtat ggcacgtcag
     8581 gtagcccaag ataaaatatt ttgttttgat tcgatgagtg catccgtcgg catcggcgtc
     8641 atggtgcgag aaatcgagcc gatgattcgc gaaaacgctc cggccgaaaa aatcatgcaa
     8701 catctggagt actgccgcaa taacatgaaa gtatttatca gcgtgcagac catggactat
     8761 ctcatcaaag gcggccgcgt aagcaaagca cgcggagcca tcgccaaatt attaaacatt
     8821 cgcccggtgc taactgtaca agatggcgcc gtagtaccca tcgccaaagc tttcggccac
     8881 tccaatgctc tgcgtaaagt gatggacctt gtcaaagcaa ccaccaaaaa cagaaaaaag
     8941 ccgcgttttg gtatcgctca cgctaactcg atcggtattg ccgattggta tgcaacgcaa
     9001 ttaagccggt attatgaaat tgaccgcgat gatatcctga tcaccgatgt atctaccgtc
     9061 atcgcaacac acgccggccc gggtgcagca gctattgcgg tattaagcga tccgtagata
     9121 ccgtgacgag ccgcgtcaat ataacgatgc ctcgaggtcc ttacgaaaag tggatcgaag
     9181 taatttgcat cagtgtcgtc gttgcctcct ttgttttggt catggggcat attcaagaat
     9241 tacccgaaag catcgccatt cattttgatt ggtcgggcta tcctgacaat tggggttcca
     9301 aacatacatt atggcttttg cctgcgttat cggctcttgt aatatacatg cctttgacag
     9361 tattttcttt tttgcaacaa ggccatacga aacccgacga catttcctcg acgttaattc
     9421 accgcaactt taaaattgca cgcataagtt tggcgctgat aaaacttaat gtgctctttc
     9481 ttatgtggtg gttaacgtat atcgtgattc aatcggcttt atcgggtcgc atagatcata
     9541 atccggtggg cgtgttatcc atttttcttt tgattttgac gctgatcatt ggtatttcca
     9601 tctatcttgt aaaagccaag cggagtaagc caagtgcata attggattat catatttttg
     9661 ttactgcatg ttatcccggt gtcgagccaa accatgagcg atccggggct aaaaaagtat
     9721 atcggcttat gggagcgcga acaaaccgat tcgcttaaaa cactcattgc ggaaatggaa
     9781 aaaaaatttc ccaaagcacc cgagacggtt tttctcaaaa acacatatga aaccgatggc
     9841 gctatcgcgt acgcggttta tctccgtttt gttcgcgact atccgaaaaa tattttcgca
     9901 ccggaagcgt atcatcgtat gatccaatac gacgccgcgg ccggccgtta tgcgttggcg
     9961 caggataatt tgattaaatt gcgaacggat tatcccggtt catcatgcat taagtcggcg
    10021 gaaacacttt ttccggcgac caaacccgac gaagcaacac aagccattat gacagccccg
    10081 agcgatacag cgcgtcctac ggaaataaat gccgggaaaa aatttcgatt gcaggtaggc
    10141 gctttttccg atcaaaaaaa cgcccaggaa cttaaacaaa aactgacagc aaaaggatac
    10201 gctccgattg aattttcgga gaaaacggtt aacgggaaaa ctttgatttt ggtatgggtc
    10261 ggcgcgtaca ctacgcacga tgacgctaaa aaagccggtg atacgatcaa aagtaaagaa
    10321 caactcaatt acagcatcgt tgaaaaatga aactatccgc gcattcattg cgctcgattt
    10381 tcctaacgaa gccttttcct ttttttcaga gcaagagagg cgtttaaagc ccttatttaa
    10441 cgacgcacgc atcgcttggg taaacccgac caatattcat atcacgttag ctttcctcgg
    10501 cgagataaat cgtgatgcta tacccaaaat cacacggtgt ttatcggatc tggatgaagc
    10561 cggccccttt tatttagccg ccacacacct tggttttttt tcgacgaaag gaaatcccaa
    10621 agttttatgg attggtttta aaaaaacatc gtctattgaa agtttgcaaa acaaaatttg
    10681 cattcggctc aatgcccttg gtttgacaac ggaagaaaaa aaaattattc cccacgccac
    10741 tttggctaga ttgcgtgatt ggccttccgc ccgccttgca cagcaaaatg tcatccatca
    10801 atttatcggt gaaacagagt gggttatgcc gacgttgact ctttatgaaa gcgtattaca
    10861 gcgtcaggga gcgcaataca aacccttatt ccaaataaaa ttcacatgaa aatcgcttcg
    10921 atacaacaaa agaaggtcaa acgtaatcaa tcggcaacgg tttgtgtttt gacagaagaa
    10981 ggtgattcat tcgaattact ctacgaaacc ttggtgaaat ttggaatata tgacggctcc
    11041 gatcttgatg aaaaaaaatg gaaccgcatt cagctttggg atagcacacg gcgcgccacg
    11101 accgacgcac tgcgttggct cagtcttcgt ccgagaagcc gcgaagaaat ttcaaatcgt
    11161 ttgcgccgcg aaggatataa tgacgaaacc ataacgtccg tactggaaaa actgagtgaa
    11221 gagaaataca tagatgattt ggattttgca atgcgatttg cgcagtcccg cctacgcaaa
    11281 aaagccatgg gtgaacgcgc cttagcatac gaactgaata aaaaaggaat cgctccggac
    11341 ttgattcggc aggtaattca aaaatttgta cctgaaaacg accttccgtc ggttttacgg
    11401 ttagcggaaa aaaagtatgc gacgattcga gaaagcgatc aacgtaaaaa acagattaaa
    11461 ctttcagcat ttttacaaca acgcggcttt gatacagata cgattcgtaa ggttgtagag
    11521 acgattgcat cacatgaaaa aataatatcc cgcgatcaaa accctctaat cccgtcaaac
    11581 tttaaaaaac attaaactgt tctattttct attgaacccg aattgtatct attcacacca
    11641 taaaacattt tgcatttatc tgcacttttg atcaaattca tgcgcctttc cacaactact
    11701 gaaatcattc gttgaacgtt ttatagtcca ccaatttttt accggaggta ttttatgcag
    11761 ctcggattta tcggccttgg aaaaatgggc ggttttatgg tacagcgcct gatgcgcgat
    11821 aaacatgaaa tcgtcggtta cgacctcaat cccgatgtcg tgaaaggtct tacagaaaaa
    11881 ggaatgaccg gcgcttcttc ctatgaagac cttgtgggaa aactcaaagg ccgtaaagcg
    11941 gtatggatca tggtccctgc gggtaagcca acagaagata ccgtcgccaa actggctaat
    12001 attctcgaca aaggagacat catcattgac ggtggtaatt cgtactataa agacgatgta
    12061 cgccgtgcca aggaactcga agccaaaggt atttgttatg tggatgttgg taccagcggc
    12121 ggcgtatggg gcctgcaaaa cggttactgc atgatggtcg gcggtaataa agccgatgtg
    12181 gatttccttt caccgatcct gacgacactt gcacccccca ataacggttg ggcgcacatg
    12241 ggcgcacatg gcagcggtca catggtcaaa atggttcaca atggaatcga atacggtatg
    12301 atgcaagctt atgccgaagg ttttgaaatt atgaaagcat ccgaatataa gctggatatt
    12361 gcacgcatcg ccgatctgtg gatgcaaggt tcagtggtgc gctcatggct tctcgaactt
    12421 accgccgaag ggctcaaaaa aaatccgacg ctcgacggta tcaaaggttg ggtggccgac
    12481 tccggcgaag gccgatggac tatcctcgaa gcgatggaga aagacgtacc tgcacccatc
    12541 attacactgt ctcttttcga acgtttccac tctcgtcaac cggaaagttt tgcagctaaa
    12601 atgctcgcta tgatgcgcaa tcaattcggc ggtcacgaag taaagaaata gttttttaat
    12661 acggcgcgtt acccaaagcc tattcgtcca acggataggc tttgttattc aacttcaaaa
    12721 acgtattcat ggataaagtg attattgtcc gagcggtacc ggaggacgca gaaaaactga
    12781 cacatatcag tcgtcaaacg ttttatgaga ctttttcaga gcaaaacaca aaagaaaaca
    12841 tgcagaaata cctggacgaa tcgttttcta tcgaacgtct taccgatgaa atgcatgatg
    12901 cccattcgca gttttatttt gccaaatcag gacacgatat cgtcggttat ataaaaataa
    12961 atctccgccc atcatcagcc gaaataaaaa atatctattc gttagaaatc gaaagaattt
    13021 atgtgcgaca cgattttcaa ggaaaaaaag tcggactttc cctgtacgaa aaagcaatgc
    13081 atatagcacg agaatcaaaa gcagatcatg tttggttggg cgtgtgggaa aagaattata
    13141 acgctattac attttataat aagctcggat ttgtcgcgtt tggctcgcat ccctttcgtt
    13201 tgggtgatga tatacaaacc gatattctga tgaagaagca actttagctg tctgttaaat
    13261 aatttattct caaactaatt tcggtttacc atataacacg taatatcatg aacgctttta
    13321 aaaaaatatc caaaacacaa tgcacctcct tgattgaaac tttaaagatc agatttgaaa
    13381 aaaacagaat tcgccatact gcaatcaagt ggacagatgt atctgtaaaa atagaaaaca
    13441 aaacaaccaa gttgttttcc ctctggaaga tggaggaaac cggaggtgaa ccggatgtca
    13501 taggctttga tataaaaaca ggcgaatata ttttctgtga ttgcgcaccg gaaagccccg
    13561 ccggacgtcg aagtctatgc tatgataaag acgcattaga cgcccgcaaa gaacacaaac
    13621 ctaaagacaa cgcgatggat gtggctgcac gcatgggcat agacatctta accgaagaag
    13681 catacaggga attacaaaaa ttaggtgaat tcgacactaa aacatccagc tggataaaaa
    13741 cacccgatca aatcagaaaa cttggcggtg cactattctg cgatcgtcgt tacgaccatg
    13801 tttttgtcta ccacaacggc gccgaatcgt attacgctgc acgcggtttt cggggaatgc
    13861 taaaaatttg aggtcagatt acttttttta gttttgtaga aaacattgcc acggcgcccg
    13921 tcaagcctgt tgttggatta tttatcttaa catgacaaag aattttgaaa cttaaacgat
    13981 ttattcaaca atatagaggc agctcattcc caaccttgat tgaaacggaa aacgcgcaga
    14041 cttacgtcct gaaaatgcgc ggttccggta acggcgctat cagccttgtt tctgaattta
    14101 ttgtatcccg cattgcatca caattgggtt ggcccatacc cgatgcacaa tggatacaaa
    14161 ttccggataa cttaccttgg acgtttggca gcgatgaatt tgacgacatc gttcagaaaa
    14221 gtttcggctg gaatcttgga attagctata taccggactc ttttccgatc acgtttgata
    14281 atcaattatt aacggatccg gttttagtat ccgctctgta tacgattgac atgtttttta
    14341 tgaatatgga cagaaccaat agcgccaata atctgcttcg ggatatccat aataaaatat
    14401 ggatcattga tcatggcgca ttggctttgt ttcaaccggg agtaaaacaa actcttgggc
    14461 ttttccataa ccatattctc tttgaaatac cttcaatttc caaaaaacaa tacaacaccg
    14521 cattacatac acccgaactc ttcaagacga ttattcatca aatacctgac gccattatca
    14581 atgaaacaca tttcagcaaa gaaactttgc tggctcgtat cataaacaga accgtcgcga
    14641 tgtctgaatt cgttgtataa attctagcct cattgatgta tgacctgctt aacgatttat
    14701 gcaaataaaa gatcattgcg aatctttgcc ttcctcacag gatgtgacta acaatcctat
    14761 ttgcatcttt aacgctgatt cggtaagttt acacgcattt ctttggatac cggttaaatt
    14821 ttcggttgtt tgcttgctta gacacaaaac ttcgtcaaaa attattccaa agacgaaagt
    14881 tacagtattc tcataatttt atagttaaaa tgcattcgtt ttgatgcatt accaaaataa
    14941 ctatgcgata catatcgcga atcaagtgta gaaataaaac ataaacagca attcgcaagc
    15001 ttttataatt acataaaaaa taataaagac aatatatatc cctacgaggg cttatgagaa
    15061 acctcaaaat cacgtataaa ctactcatcg gttttggcac gttgctcgcc atgttaatcg
    15121 gcttatccta ttttgcgatc agcaaaatgt ccgatttgtt tggcatgtcc aaagcagcaa
    15181 tagatgtgat acctgcgcaa aacgctattg tcgaaattca atatcatacg accaaggggc
    15241 acctttggtt tgaggaaatg ttgtccggtg ataattcgat tcgtatggag gaagtatatg
    15301 atcactggaa tggcggcatc gaatatgcat ctgtattatt ggatggcggg actcgtgata
    15361 atcaaacgta ttccgctgtc aaagacacgg ctttgattga gatactcaca agcatacggt
    15421 atgcattggt ggatatggtc aatgcggcgg atgaacgctt agaacttaaa aaacaaaaaa
    15481 taaaatcaga agccggtacg gagtcggaca tctattttga tgcagcttat cagcgtgtca
    15541 tggattactg cgacaacgga aaaaaaatca cgaccggact tatcaataaa agtgtccgca
    15601 atcttgaaaa tgatttttca agttcccgat ggacattgat tatagaaacc acggttattg
    15661 ttttgatcgg cattttgttt agtacgctca ttgcccggac tatcgctttg ccggtgcgtg
    15721 aagcggcgct caagatcaac cgcgatggtg tgaatacccg gtttgacatg caacgtaagg
    15781 atgagatcgg acaattagta caaaccgtgg atagtttctt aagttcattc cgcatgatct
    15841 tgcgcgaagt agccgatgcg agcgccgctg tatcttcatc cagtaaacag atcggcgtga
    15901 gtacggaaaa catggcgcgc aatgcacaag atcaaatggg ccaaacagcc agcgtaaccg
    15961 ccgctatcga agaaatgagc aaaaccagtt atgagatcgc tcacaatatt ggcaatacca
    16021 aagaaatggc tatccacgca caaagtagcg ccgataaagg ttcgcaagtg attgatgaaa
    16081 cggtggacgg tatgcaaaaa atgctggcca tggttcagca gtcttccaac gttgtcaatt
    16141 ctttatccga atccagtcga aaaattacta aggtcgtaag catcattgac gacattacta
    16201 aacaagtgaa cctgatcgca ctcaacgcgt cgattgaagc gacccgcgcc ggtgaaaaag
    16261 gtaaaggttt tgccgttgtg gccgatgaaa tcaaaaaact ggccgagcgt acggctcatt
    16321 ctaccactga gatcaatgac atcatatcca agatacaggt caacgtaaac gaagccgtca
    16381 atctgatggg acgtagcaat gaagaagcag ccaatggcat acgtctttca caagaagcca
    16441 agtctagctt gaaggaaatt tcagacgtat tcaaaaatgt caccatgatg gtcagtcagg
    16501 tagccgtctc aagccaggaa caatccgtta cgggcgatca gatcacggat aatgtcaaag
    16561 tgatcaatac ggcaacacag caaatcgccg gcggtatcaa cgaaatagcc aagtccgccg
    16621 aatatcttaa taaccttacc acgacacttc aaaaaactgt taagcgtttt aaaatcggtg
    16681 attaaaaata ttcatcaaat tctttttgat tttgactata cgctcgctga ttcttccgaa
    16741 ggtatcatca cctgtgttaa ttttgctctg gaaaaagtgg gcgccccccc cggacagccg
    16801 gacgccattc gccgtatgat cggccattcg ctcgaaaata catttgctcg ttttattgat
    16861 ccgtcggata cggaaaaaat acaaacctgt aaatctcttt ttatggagca tgccgatacc
    16921 ggtgctatgg ttcgcaatac ggtcatgctg gacggcgtac gtgaaacatt ggagttctta
    16981 tataacgaac tttatacatt gggcattgtt tctactaaaa gacgctccac gatatgggaa
    17041 acactgatcg aaaaagaaat ggatgatttt ttcgatattg tcatcggata cgaagacgtc
    17101 acgcatctca agcctcatcc ggaaggattg atcaaagcca ttgaggaatt agccggcaca
    17161 cccgaaacaa cgatttacat cggcgacagc cctttggata tcaaagcggc aaaatctgcg
    17221 aatgtttttg ttgtcgccgt aacatccgga atgcattcga ccgatgaatt agcaaccttg
    17281 aatccggatt tgatcatccc acgtctctct gatctcaaag cgcatttggc ggcatgaaga
    17341 aaatcggata catatttttt ggtatgaatt ttattttgtc gtgcggtgcg cacaatgtta
    17401 aacaaaccgc atcggtagag ccgtccccga tcatacagcc catttctcag caagaccatt
    17461 cggaaaaagc gtttgatttt acaatgctca atgatccgtg gatcatcgaa ccgcgaacat
    17521 acgaagtaac cattcaacct tataccgaac cggccgaagt tgtagttaca tcttccggca
    17581 aagaaacctc tgttatcgga taccgtgtgc aacttatttc cacaacggat tattatgaag
    17641 cattggaaat aagagactcc gtaagcgttc gtttgagcgg cgaaatttat cttgattatg
    17701 aaccgccata ttataaagtt cgcgcgggaa attttataca acgcgacgat gccgaaatga
    17761 agaaagccga agccaaacaa gccggttatc ccgacgcgtg gatcgtccgt acaaagatca
    17821 tacaaaaaaa ataaacataa ccatccaaga ggagtattcg catggaagtc aaagacatca
    17881 aaaccgtcgg cgtgttgggt tgcggcctta tgggcgccgg tattgctcag gtcgccgctg
    17941 catcgggatt taaaactatc gttcgcgaag taaaccagga cgtgattgat aaaggcgtcg
    18001 gaaatatcaa aaaacaattg gaacgtaatg ccgccaaagg taaaatcacg gccgaagaaa
    18061 aagataaaac catcgctaat ctcaccggaa cgaccagttt agatgatctg aaaaactgtg
    18121 actttatcat cgaagccatt atcgaagacg taaaaattaa aaacgaaaca tatgcttatc
    18181 ttgataagat ctgtcctccg catacgattt ttgcttccaa tacatccagc cttaccgtga
    18241 cggaaatggc cgccgcaacc aatcgtcccg accgtttctg cggtatgcac ttttttaatc
    18301 cggtaccggt catgaaactg gttgaaatcg taaaagccgt acgtacgtcg gaagaaacat
    18361 ttaatagggc tttcgaattg ggcaaagcgt ttggcaaaac accgatcgca acaaaagaca
    18421 attccggatt cgtggtcaat ttccttttag tgccttatct cctcggtgcg atccgtgccg
    18481 tacaaaacaa catcgcttcc atcgaagata tcgacaaagg tatgatgctc ggttgcggtt
    18541 atcccatggg cccgctgacg cttttagatt ttgtcggtat tgacacgacc taccgtatcg
    18601 ctgaaatcat gttcaaagaa tacggcgaac cgatgtattc ttgccccccg ctgcttcgca
    18661 aaatgttttt agcgggcttt tatggccgta agaacggcaa aggcttttac gattattcat
    18721 caggaacacc cgtcgtcaat aaagacgtga tgaatttcgt taagtaattt aaaatcaaaa
    18781 ttacgatata aaaagaggct gtcttaagat agcctctttt ttttggcctt tgggcttggt
    18841 tcgaaataca aatttatttc cgaaactttt tccgccgaat ctcgttttat aaaaagtcca
    18901 aaaaaacgag aagggatgta tatgaaaaaa ttatttctca cgctgacact gttggcagcc
    18961 tttactgttt cggcgaccga taacacagcg gcatcgggtt cggtcgttgc ggttaaagaa
    19021 tttacacgtt catttcccgt atcaccgggt ggtcaactta ccttgactac agactttggt
    19081 tcgatcaaga tcgtgggctc ttccgatgac aaggtttcta tcatggcgcg tatgcgcggt
    19141 tcggatgacg atcttaaaga ttttgaaatt acggcggaaa aaacgggtaa cgatgtgact
    19201 attcgcggca actggatgag aaaaaacaat ttctttggcc ataatagtaa ctcaatggat
    19261 gttgattata tcgtcaatgt tccttcgcaa tataatgttt ctttaggcac ggccggcgga
    19321 aatatcgtca ttcaggacgt aaaagggtta atccaaggtg ggacatccgg tggtaatctt
    19381 gatttgacga atatcaatgg taatgcagaa ctcaaaactt ccggtggtaa tattcgtgcc
    19441 aaatcaacga caggcaatct caacatgaga acctccggtg gaaatgtgag catgagtaat
    19501 attaacggcg atttggatgt ttccacatcc ggcggtagta tccgtatcga agacatcaac
    19561 ggtcgcacca atgctgaaac atccggcggg catattgagg tgtatgtgag cggtaataat
    19621 aaaggcgtta atgtggaaac atcaggcggt cacatcgcgc tgtatctccc caaaaacatt
    19681 gccgcaacga tagacgcctc cacgagcggt ggcgaggttg agtttgattt accgatcatg
    19741 gtcaacggga aaatgcgcga aaacaaagta cgtggcacaa tcaacggtgg cggcgaattg
    19801 atacgcgcac acacttccgg tggcgacata cgcattctta cccgaaacta aaatcaaagg
    19861 tttatggaac aactgataga aatcttaaaa cgactttttg gaaagcaacc tgaacccgta
    19921 ccggtaccag ttccgagaaa gtaaactgta tagctaaaaa accccgaagt atttttttcg
    19981 gatcgtaaag aacgacgtaa aaatatttcg gggttattcg tttccaaatg ctacaaccat
    20041 cgccgcaacc caccgggtaa cattctctaa aaccttttca tcaatctttt ctgaggtatc
    20101 gccgggtgca tgatagggca tcgtgccacg ataggcatac acaaaaaaag ccggtaatcc
    20161 tattttgttg aaataatagt ggtctgagtt cggcgaatta ggtcgcgtac tcatttcaaa
    20221 gccaaaccgc tcatcattta ttttttttat acgcgtcgca taatcgggat agttttccga
    20281 tccttgtaac atgataccgt cacgacccga accaaccaga tcgagattaa tcaacaggcg
    20341 gattttgtta agatcaatcc atgggttttc tataaaatga taagacccta gtaaaccggc
    20401 ttcttcgccg ctaaacaaaa taaaaaccac agaacgcccc aaatcggttt tggattcttt
    20461 gatccatcgc gcaatctgca acacggatgc aaccccgctc gcattatcgt tagcaccggc
    20521 atagatcgaa tcaccttctt ttcccagatg atccaggtgt gccgttaaaa caataaactc
    20581 attgcgccgg agagaatcat ttcccgctat atacccgata atgttttttg tcgaagccgt
    20641 ttcgcttgac cattcgtttg aatgcagccg ttcttttaat acagaagtcg ataccgtaaa
    20701 atcttgaaaa tatcctccgg gtaaaggttg aatgccgtgt tgaacaaaaa attctttcac
    20761 ccatcgtgcg gctttatcaa atccacgtgt tcccgtcgcg cgtccttcca tagaatccgc
    20821 agacaaggcg gccaccatat ctttgattgt ttctccctcg atgtgcgcca gatcggaata
    20881 aacgcctgat ccgggccgca atatcgcctg atctaaagga cgtacctgcg ctgaaagcgt
    20941 gaagctatat aataaaaagg cggaaataat tccgccgtgt atgatcaaac gatagttttt
    21001 tattttcatc ggctaaaaac ggtaacttaa actgagcaga agttttttag atgttatttt
    21061 ttcagaggtg taccggttat aataatccgg cgaaggcccg taggccaata aattatcggt
    21121 tgttgcctgc ttccaccatc catattggta agcagcgtct attttaacct gttcgtctaa
    21181 gagaaaactt gcaccaacag aaatataacg gtgatcgcta ttaacgatct caaggccatt
    21241 tttataaggc gattgttggt aagcgtatcc ggccataact ttacaaccga tctgagggac
    21301 taccatctct gtgccgatac gataatttac cgatgtacga taatcattga gtaatgcgtt
    21361 gttgatcgca tcgtctatat tttttccatc ggcatcaaat atattagaac tgaatcgtgt
    21421 ttgttgccaa tccacccatt cgacagagcc tgtaaaaagg gcaagtttgt atttgtatga
    21481 actgccaatc tcaaatttcc aaggcgatgt aatatgatac ttgtagggct cataccatga
    21541 atccgaataa tatccgttgt tgtccgtttt gatggtcgtg gattcggagt aattttctga
    21601 aacggtgatt tttgtaggtg ccgttaccgt gagagcaacg cgcaggtttt ttgcggggcg
    21661 aaatagagtt ccgattttga agttaaaccc cgaatagtcc ggtttttcag cgaaggatac
    21721 ttcttttgta gcaatgctgt catcaagcat ggcaatctca tcatcctgaa aaaaatctac
    21781 acttagatca tttttcccag taacaatatt gagtgttgca ccgactgaaa cattgcgcgt
    21841 cagatcaagc gcaccggcaa aactccaatg gttaaggcta ccggtatttg tagttttttc
    21901 atcctgatca aaacttatat taccgatcat cggcgaaccg gtcacgcgat tgtaatcgtc
    21961 aaatgagttg acgcgatgaa aaccgaaagc caacacaagg cttccgcgcg ttgtgggaac
    22021 cggaaataca aatcctaaag cgttcatgct tgtaaaaccg ttggatgtct ctgtacgcgt
    22081 accttggtat gtgatggcat tagtattatt tcgatgcgca aacgctgtat aaaattccat
    22141 tttacgtatt tgcgcaagcc ctgccggatt ccaatatacg gcggtataat cttcggcaat
    22201 tgcagaataa gctccaccca aacccaatgc ccgcgcaccg ggaccaaatt cgttcataaa
    22261 aggccgcacc gcattatttt cattttgtgc caataaaata aatgaataaa aaaataaact
    22321 tatcccatac gataaaaact tcatgtacat cacctcagtt atttcttgtc tccgcttttc
    22381 gcagttctgc gatttccgta acctcttttt ctgttcgtat cttgagtctt cgtggtcgaa
    22441 ttagaattag aatccgaact accggatccg gattgtactg tagagtctgt tttaaccgga
    22501 gtggatgggc tactatagga agcgcttgat ccggtagaat acgaaggtac aaacgacggc
    22561 gtcgttccac ccgggctata aacgggtgaa gcataaccgg tcgaaacaga agacccggac
    22621 gacgcgtcat tgagcgccgc acgacgtctc ccgtaatttc tgcgtgatga cgaatacgat
    22681 gatgtttcgg taggatcagt agccgttgtc gtatcgtcaa acgcatcgta ttcatccgca
    22741 ttcggcacat accaccacgg gaacggtgtt tcgtaatagt attcccacat cgtcgaaggt
    22801 gtaacaccat acggttcgta agccgcaaaa tcgggttcgg attcggttgc ttcgttttgg
    22861 atacgatcat ccgaacggga aataccaaca acggtataac acgcactacc gtacaatgac
    22921 acaaggattg tgattaaaag accaacgtat ttcataaatt aatttctcgc cgaacgtttc
    22981 ccggaagaac gggtttcgcc ggatcgacct tcactacgac cggagcgtcc gctgtttctt
    23041 tcaccggaac gcgcgttacg ttctcctccg ttattgcggg aattattatc gcgtacttgt
    23101 ctggtttcac gtgtttcttt attacgcgtg cttgtttcac gattggcatt gcgggattta
    23161 cggccagctt ccgcattgac agccttatca cccgtttcaa agccgtaata tttacggtag
    23221 tttcggcgca cacggtcttg gtcaattcta gactcaccgg aggtgcgatt gccggaacga
    23281 tagatgcgct gcgttgtgcc tccgcctgac gaggctacac tgacccccac aataccggga
    23341 agattaggcg ttgacgaggc agtatagacg cgtcgcccac catacgtacg ctgttgtgac
    23401 ggatgtatcg gattattatc atgatggccg taaccggtcg gataataaca ccatccgtcg
    23461 tagtaataat acggatcata aaaaggatca taataaccat aaccccaata cgttccgaca
    23521 taggccagtc gaacacgcgg accaaaatac atcccggcgc cgtaataata cggatgacga
    23581 taatttaccc aacccggtgt ccagtaacca taaggatatg gatattccgg gccataatag
    23641 ttttgaatat aaatatcacg atcttcgtcg taggatggag tgacttcttc atcctgatcc
    23701 gatgagtctt catccgatgg ctgaatttga gttatctccg gttgttttgc atcggcatga
    23761 tcaaactcgg agtaataccc ccctgattga cgtacagccg tataacatcc gctccaaatc
    23821 agcgaaaaaa ccaaaaccgt gagtattttt ttaaaagagg ctttcataag gcaccttcct
    23881 tacgatccta gaaatatcaa ttcttataaa ctattagatt atacgacctt ttttgtcctt
    23941 gtaccacgaa aatcaaagcg attatttaca aataattacg ataacttgta tgccgttaaa
    24001 tatgattttg tcggcattca ttagacgtat agtatttaca aaagtttaac agcgtggtca
    24061 ctttagatat gctctataac ggtctttttc attacttcga tcggcgatcc tttggccaca
    24121 aaaacagaat ccggattacg ggccattgtt ttggcaaagg gttcgccata cagacctccg
    24181 atatcacatg cgatatggta atccatcaca ggatatatat cccaacgcgg atgccgcact
    24241 tcgtaagcgc caagtttttt ttcgttgatg cgagtataac cccaataatg ttcggcaata
    24301 aactcctgtt ccgaaccttc agccatcgcg attgattcgg tattcgtttt cgctgaaatc
    24361 gtattccagc cgttagccga tttgaaacta tactcaaatg ttttacagtg cgcatcggac
    24421 gtaaaacggt gccgcatcgc gtgcgtcgca taatgctcat gatacactgt attggcgata
    24481 catgtgatga gactgcgcgg aacaatctct ttgataaaaa caacgccgcg gcgccataca
    24541 ccggattcaa atcgccgtac ataaaatctc agattgactt cttcgaatgt ccggtgaaaa
    24601 ggaaacgaca gaccgtgtac acgtgtatct tcaaataaaa atcccaccaa gctgacataa
    24661 aatgtgtctt tccaggaatc caattccgtg ccgcttggca catacggccg gagcagcgac
    24721 gggtcaacgg catagttggc catgataagg tttttccatt gagccctgag aaatgcgtcg
    24781 gacataaaat ctcccgtttt gttcggaagt gtatgctaca caaacttatt cagagaggca
    24841 agccgggtat ttatccgctg taactgcgat ccaaacttcg gtattggatt gcttcgctga
    24901 cgtgttcggc gcgaatggcc ggagcattgg cacaatcggc aatcgtccgc gctactttga
    24961 ggatacgatc gtatgcccgt gcagataaac ccagtttggt aatcgcgagt ttcattaatt
    25021 cctgactgcg tgcgtcgatc gtacaaaatt cgcgaatggc gcgcgactcc atatccgcat
    25081 tgcagtacac aggctggcct tttttacgca gagattcaaa tcgttttaac tgaatttgcc
    25141 gcgctttttt aacacgttcg cgaatcgccg aggaggtttc gccgtggcct tcggaagaaa
    25201 gttctttata ttttatcgcc ggacagtcaa tatgaatatc aatacgatcc agaagtggcc
    25261 ccgatatttt ggccatgtag cgctggatca tacccggatt acaagtacag cgattgccgg
    25321 gatcggtgtg aaatccgcac gggcaaggat tcatcgcgca agcgagcata aaattagccg
    25381 gataggtcag cgacatttca gcgcgactaa tggtaacatg cccgtcttcc aaaggttggc
    25441 gcatgacctc caatacgttt tttttaaatt ccggtaattc atcaagaaaa agtacgccgt
    25501 gatgcgcaag actgacttca cccggtttgg gaatatgtcc cccgccaatc agtcccgcgt
    25561 cggagatcgt atggtgcggt gaacgaaaag gacgcagggt aaccagcggc gtattctgcg
    25621 gcaaaaaacc ggcgacagaa tgaattttgg ttgtttccag cgcctcatcc ggtgtcagat
    25681 caggcagtat tgtcgggata cgtttggcta acatcgtttt accgcttccg ggcggaccga
    25741 tcataatgat gttatggcct ccggcggccg caatctccag cgctcgcttg gcatgttcct
    25801 gccctttgac atcacgaaag tccaccggca tagccggagc ctgatcaaaa agtgccgaca
    25861 tatctacctg gtgcggtgcg atgtgttttt gtccgttgag aaattgaaca gcatccgata
    25921 atgcgttgac aggtataact tctaaacctt cagccatggc ggcttcggcg gcattaccgg
    25981 ccggtacgat gatacctttg atatgttcgc ggcgggcgcg aagcgcaaga gacaacacac
    26041 cgttgatggg tcgcaaactt ccatccaaac tcaattcacc gacgaccacg tattcctccg
    26101 tacgatggga ctcgatctga ccgtgcgcgg ccaaaatacc cacggcgatc gggagatcaa
    26161 acccggaacc ttctttgcgc aaatccgccg gtgcaagatt gatcgtaatg cgtttgatcg
    26221 gaaatgtaaa accggagttt ttgattgccg cattaacgcg ctctttgctt tcgcggacgg
    26281 cattatccgg taaaccgacc gtaataaaac cgggtacggc gccctccaga tgcgcttcga
    26341 ctttgatgat aaaagcatca ataccaaatg tagtcgcgga taaaatctgt gctaacataa
    26401 acccccttca tcgcaaattt ttttaaaaaa attaaatatg gatgcggaag gttttctgtc
    26461 aaaaaatgac aataaaaaaa cggcttccgg gattttttaa ccgaaagccg tttaatggga
    26521 cgaattgtat tttttagttt ttgattacag acggtaaacg acccggcgta aaaggcgcgc
    26581 cataacgatc catctgctgt tctatgcgtt tgatctccgc atcagtctcc tggagtgcac
    26641 gaagtaatcc gtcaaattgt gcggccgcca cgtcatacaa atgctgaaat gtagttgttg
    26701 catccgatgt ggtagtccac agattaccgg tgatgatttc gacgcgcccc cgcaacgatg
    26761 tcggcggtac accttcatac agcgcacgca atccgtcacc attcatttga cgttgaatac
    26821 gtttgagttt atcctctgca gcgaccacag ctgcatacgt tccttcggga agctttgccg
    26881 tttcgatcac ggcttgtttg agcaacggta ttttctccac catactctca cggtaggcat
    26941 ccgcactgct gatagcccgg atcagttcgg ctactttttt attgaaggcg tctaatttat
    27001 tgcgatccgc aacaggcaaa gacggtgtca aaagcggttt acaaataaag gattgcgaca
    27061 cgccgagatc cgtccattga ccggcttcgt agcgcgacac ggaaaccgtg tattttcccg
    27121 gaaccaccat atatccccga tccggatcat tccaaggtat gctgttatca aatggcgcta
    27181 gagaaatcgg ggtaaataca ttggttcgaa aatcccacac gatacgattg acgcctttct
    27241 gaacggaagt tttaatgcgt cgtacaggtt gacccaattc atctttgatc gtaaaaacaa
    27301 aaaacggttc ttgctcatcc gcttcattgc gcagtgtttg atacgaaggc agttcgatgt
    27361 cctcgccttt tttctgtttt tctttttcgg tatcgcggcg ttttttcttc agcgacttgt
    27421 aatcgtcctt aacgtaatac gtaaatacgg cgcctgtttc cggattcggc gttgcaaaaa
    27481 aaccggcacc ctgaaaaccc gttccgggaa aaccaaacgg attggaagga ataaacatgg
    27541 atgcctcttt gatcggcagt atggcaatat ctttttgtaa cgtttctttg gacaggtgac
    27601 ggagcggcgt ataatcgtcg agaatataca taccgcgacc aaacgtggcg acgaccaaat
    27661 cgttttcacg tttttggatg gtcatgtcgg tgatcaatcc cggaggaata ccattgttaa
    27721 acgctaccca gtcctgacct ccggtattgg aaacatacag accaaacatg gtacccacga
    27781 ataacaaatt aggatccacg tgatcttctg caaaagaata ggtagaacct ctcttgggaa
    27841 gattgccgtt gagtaacgtc cacgtcttcc cgccatcgct tgttttgagc aagtacggtt
    27901 tattatcgcc gtcaccaaaa ttgtggcaag cggcatacgc cacgcgtctg ttatgcgacg
    27961 atgcgacgat cgtatgaacg cgtgcaaact ccggtaaccc tcccgtggat gcgggtttat
    28021 tccacgtttt accgccatca tcggaataat gaatcaatcc gtcgcccgaa ccgacgaaaa
    28081 gaatattggg atctatcggc gattccgcga tggccgtaat attggccagc gaacctttag
    28141 aggccagctc atcataactc cagctcttgc cctgcatttt gattaatttt tgcggaacac
    28201 cgcgcgtcag atcgccgctc agcagtgtcc acgtattgcc gcgatcgtct gttttaaata
    28261 aacgatgacc gccgaaatac aatcgtttgg gatcgtgatg tgaaatcaat aacgccgcat
    28321 cccaatcaaa acgataaccc acatcggccg tatcaatggg tttgataaaa agttgttcac
    28381 ccgttttttt atcataacgt acaagcccgc cattttggga ctgcacataa agtatgtttt
    28441 catcactcca gtcggcttgg gtttcaaatc cgtcaccgcc taaagtaaac gtccaatctt
    28501 gattggtcac tccggccgat gaattcgttc ttgatggtcc gaagaaactg ttattgtcct
    28561 gcgtacccgc atagacatga taaaacggga gcgcctgatc tatcgaaact ttatacactt
    28621 cggcaatggg catattggct ttaaaatccc aggttttacc ttgatctaat gattcgtaca
    28681 ctccgccgtc acttcctgca atcaggtgtt tggtgtttat aggattaatc caaagcgcat
    28741 gattatctac gtgtttgtat ttctcgccaa gtttggacca agtcgcaccg ccatcacggc
    28801 tgatctgatt aaaaacatcc atgctgtaaa cggtattctc atccttggga tcgcaaaata
    28861 attcctgaaa ataaaacgca tacgtcgaaa cataactgct gcgtttgacc cagctcgcac
    28921 cgcggtcgct gctgcggtac acaccgcctt tttctttggc ttgcacgata gcataaagca
    28981 cttgagggtt ggaaggcgct atcgccagtc cgattttgcc cacatcttcg gaaggcagtc
    29041 cggacatcac tttttgccac gacacaccgg cgtccgtgct gcgatagatc gcactctccg
    29101 gcccgccttg aacggtagcg tatagttttt tgaaccgttg atgcgccacg gcatataaaa
    29161 ctgacgaatt attcggatcc atatggattt gccaaaatcc cgtatagtcg ctcacatgaa
    29221 gtacacgtgt ccaggtttta ccgccgtcgg tggttttata aacaccacgc tcaccaccgt
    29281 cgttacgaaa cgggccatat gccgccgcat atacggtatt ggaattacgc gggtcaataa
    29341 cgatcccgcc gatatgctct gaattttcga gtcccatatt tttccaactt ttaccgccgt
    29401 cttcgctgcg ataaataccg tcgccaaaaa tcacattgga ctgtccattg ctttcaccgg
    29461 tgccaaccca cacgatattg gaattggacg gatccatcac cacacaaccg atagagtacg
    29521 atttttgccc atcgaatacc ggcgcgaacg tcgtcccgcc attagtggtt ttccacaatg
    29581 acccgtgacc cgaagcaaca aaatattccg ccggattggc aggattaacc gcaatatcta
    29641 cgatgcggcc gccggcaaaa gccgggccga tactgcgaaa ctgcagtccc gataatgaaa
    29701 tgttcttgag cgaatccgct ttggtttgcg cttccaacgc aaaactcatg cagcatgaca
    29761 caatcagaat aatacgcgtg atatgattca taaaaaaatc ctcatgcgat gggttaaata
    29821 ataatgatta cttgatatga caaatcaata aagcaatgaa accgagatac caaaaaagaa
    29881 aggacatagg cacgtcatgt tacgaaaaac acaacttata gtttcgttta aatgctggtt
    29941 tatttttaaa ataagtctat gaatttttaa atatgagaaa agaagctatt acgattagca
    30001 ttttaatacg aataatccga ccgatgatgt ggaaagccgc caacctgaga atacatggta
    30061 aagggaaaaa aaacttgact tttttgagtc cgaacgctat tattgccccg ttcgatgaac
    30121 actgctcagt agctcaatgg cagagcattc ggctgttaac cgaagggttg taggttcgag
    30181 ccctacctga gcagccaaac ccgcgtactt gcattaggta tgcgggtttt ctattttgtg
    30241 ccaacatttt ttgaaagcgt cgttatgcga aaatcgttgt tagggttctg ggcattattc
    30301 atcattccct tttcattata tagtcagttg accggatttc atctatacga aaaagacggt
    30361 gtttataatg tttcaccctg gctccgttac gaccgcgcat ccggattttt caccggcttg
    30421 acgggcgatt atttttttac cgaacgcctc caaatgcacg gtgaaggagg atacgcttgg
    30481 gcggcacgaa aacccgatta ccacctccga ttacagcaga gttttatatc agataatacg
    30541 gaatatagta tcgccgcggc atattttgat aaagccgata cgcgcgatcg gatggtttta
    30601 ccggaatggc aaaattcttt gacatccata gtggccaaac gcgattatta tgattggtat
    30661 cgcatcaaag gcggaaactt cgaaatgcgt accgatattt ccggtacact caaaatcacc
    30721 atatctggaa cctggcaaca ctatcgcaat gccgatgtaa acgcgcaatg gagcatgttt
    30781 gattggggtg cgaaaaagaa caatggccac gcgcgatttc gtataaatcc ggaaattcca
    30841 gaagggcgtg atgtgtcact cggtacggcg atcgaattag acacccggcc ttcgcctttg
    30901 gcatcggtga gcgcatggtt gatacacagc gactatgcca atgcccgttt actgcacaaa
    30961 gcgttcaatt cggatttttc ataccagcgt gggagcctga ctattacgcg ccaacagcgc
    31021 atgtttggaa aacaaaaatg gacattctac ggcgcctatg acggtttttc agggaacacc
    31081 gtcattcaaa aagactcttc ctcgtcgctt acttacggcc agtttttata cgatgccggc
    31141 ggcttaggca gtctgcgcgg atatggttat aaggaatttt cggacggcaa tcggcgcatg
    31201 cttttccaga gtgaatacta ttttaatggt tcttttttgc cgcaaactcc cctcaccaaa
    31261 atatggggcc tgggttttat atttaaaaca tttgacctga tgttattttg ggatatcggc
    31321 cacgcatggc aagcctcgga aaaagtcatg tatttcgtac ccgatggttt tcattggaga
    31381 gatatgagat catctgcagg cggtggtttg gccgtatcgg attggatcaa aatacaatat
    31441 gcttacgttt taaaagacgg tatccgaacc catcgaggaa attacggaat ctatttccaa
    31501 ttcacaccca agctaaaata atcgctatga tagacagcca gtattttttt gtccggccgc
    31561 aggatgtgta tgccgatcat ttggttctgc gagatgacga atcgcaacac tgcgtcaaag
    31621 tgttgcgcaa agcgcccgga gatcgtttct atgcgataga cggattgggg cacgaattta
    31681 cagttgcatt ggaacattcc gatagaacat cagcccactg ccgtattctt tcgacagcga
    31741 gtaaaccccg cgaactcgga tttgaaatta cactggtaca ggctttgatc aaaaaagatc
    31801 attttgacct cgtggtggaa aaagtgaccg aactcggcgt acatcgtatc atccccgtca
    31861 agacgcgacg ttctctgccg gaggcaggcc acacaaaaat agaacgttgg cacaaaatcg
    31921 cattgaccgc catgaaacaa tcgcgacgaa gcctgatgcc ggaaataatg ccggtttcag
    31981 atttaaactc cgtattgcga aggtacgcgg atcgccccgt tcaaaaaatt tttctgcatg
    32041 aatcttccga acaacctctg gatccaaacg gaataaattt tgcgaaagcg tgcgatacca
    32101 taatcgtcgt tggtccggag ggcggtttta cggacgaaga agccgctatg gccgcagagt
    32161 atggattcca aagtttttcg ctgggcttac gccgtttgcg tgctgaaacg gcaagcattt
    32221 gtggtgtagg tcttttttca atgtttgcac acccgtaaaa tatctaaaaa cgggctttta
    32281 ggggcatata tacccgtaat catgccacta tcaaggcttt atacgaaaat acatcttgac
    32341 aaaagccact tcacgatata tattaatatc gttacaaaaa aacgatctgt tttcgattcg
    32401 aaatcaaaaa cagacgcttt caaaaaagtt ctttcccaat actaaatttc atttcacgat
    32461 cgctctcgat ggttagactg cttaagtttt gagtgcgtat gattaatcaa gtacgtgctt
    32521 ttcaccaaaa aaaggagttc taaccatgac caaagctgat ctcgtcgcca agatggcgaa
    32581 agacgctgat gtatcgaaaa ccaaagcaga aaaatctttg gaatcattca tcgaagcgat
    32641 ctccatgtcc ttgaaaaaag gcaaagctgt aacgctcgtt ggtttcggca catttagcgt
    32701 ggcaaaacgt aaagcccgta cgggtcgcaa tccccaaacc ggcgctacga tcaaaattgc
    32761 tgcggctaaa gttccgaaat tcaaagccgg caaagccctc aagcacgtgg taaaaaaagg
    32821 ttaatcgctt tcactccctc cgtaataggt gaagaaagcg gtcacctaaa gtctctcgga
    32881 tataccggga gactttttta ttttttggct tgattgcctt accctcatcc catacatttt
    32941 gccgaatgaa aactatgttc tcatgtctaa actaattctt ggcatcttag cctccggcaa
    33001 tggctccaat tgcagggcta tccataaagc gataaccgat ggattgtgta aagctcaaat
    33061 cggtgtaatt atcagtaatc acgcggaagc aggcgttttg cagtttgctc gtgagaacgg
    33121 tattccggct tttcatatcg ccagtgaaca gttttgtgaa atttcagaat ttcatgcagc
    33181 gatcaaaaat tgtttatcac agcatcaggt ccatttggtt gttttagccg gttatatgaa
    33241 aaaaatcggt aaaccgattt tagatattta cccgaaccgc attattaata tacatcctgc
    33301 acttttgcct gcatttggcg gcaaaggtat gtacggacat cacgtgcacc aggctgtttt
    33361 ggaatacgga gcgaaaatca ccggtgttac ggtacatttc gtagaccctg aatatgatca
    33421 tggtaagatc atcctccaaa aggctgttga agtgatggaa gatgataatg tggagtcctt
    33481 gagtgctcgt gtactgaaag ccgaacacga aacttattat aaagcgataa atcttttcgc
    33541 taacgagtga tataaaaatt tactttcaaa tactattgaa ccgaaataac aggagaggca
    33601 tgatgaaacc gataaaaaat gttttatggc tactgatagt aggcgcaagt ctgttcgccc
    33661 aaggcaaaac cagcattgac tcggacaaac aaaaagtagc tattatcccg attgatgcag
    33721 ttaaaatcgc cgaatcgtat ggcgctacac aaaccgtgaa ctggcaattg agcggagcga
    33781 cgtggaaatc ggctcccgaa tcaccttttg ctgatccggc atcccttgct gcttttgccg
    33841 aagctgccac gcaacgcgtc atcgaagctt ttgtaaagct tggccgtttt gtcgtgcttg
    33901 accgtagtgc gatggaaaaa ataatgaagg aacaggattt ccaactttcc gataatgtcg
    33961 atccgaacac ggtgaccaat atcggcggac ttttgggcgc acaatacatc gtgaacgggc
    34021 aattacaaca agtcagtacc aatccgtatt ataatccgaa agataaaaca acactgatgg
    34081 gctattctgg tacggtagaa attcagatta ccatcttaga tgtggccaca ggtcaagtta
    34141 tgacatctaa gcgcgtcaag ggcagtacgg aagtcgaagg aacaaaaatt ctcggtatgg
    34201 cattattgga cgcttatgaa tccacaccga tcaaggctgc atacaaaggt cttagcgaat
    34261 gcggcagtga cctcaaaaaa tggctacgcg atgcattccc tgtcgaaggc gagatttttg
    34321 aaattactaa agaagacaag aaaaaaggcg ccatagtggt taatattacc tgcggcaaag
    34381 atatcggcgt caagaaaggc gatcgttttc gcgtctatac agaatcggaa gtggaaatag
    34441 ccggaaaaat gcgcaaaaaa accaccgatg tcgctactct cgcagtaaag actgttgccg
    34501 aagacggttg gtcatcgctg tgtgatgtcg aaaaaggcgg taaatccatt tctgaaaagg
    34561 tcaaagccgg tgaaaaactg aaagtagttt cggttaaaaa ataacttcaa ataaatatct
    34621 gaataaaaaa ccccgaacac actcggggtt tttttattta tcaaaactat caatatctct
    34681 atttcacgca tccatcacgg ctttgtatca atcgtatttt tgaaatcctt tagcgctttg
    34741 ttaattgcca tttcattgga taagaccgta tccaacacag caatcatgga ttcaaatttc
    34801 tcatgatcgt gaagtatctg gtaattgttg cgccagcgtt tatgagcgat gagcaattcg
    34861 ttgtatgacg attttaatgc ggcatcggtc gggcgaataa ccccgcgaag gctatccaga
    34921 taagctgtat ggtacagttc ggcatttcta taattttccc gcgcgatatt gtttcgcacc
    34981 gcctcgcgcc acggctcgcc ggaacgtttg gcaaagagta tgagcccaag ccgttgattg
    35041 aaatctttac tccgattgcc caattcaata ctgaaagcga ggagttgctc tctaaaaggt
    35101 aacgctttta ccgaagtaac aggacgaccg gcttcaatag gaatatcgta gttgatcggt
    35161 tgtaccgctg tggatgaact atcttgacca agctgtgatt catccgtttt tttcttcccg
    35221 cagctcgcga tcagaagaac taaaaaaaca aatagtggaa atctcaaact catcccttca
    35281 ccaccacatt gactaattta tccggtacca caactatttt aacaatttct tttccgccaa
    35341 cccaatgtcc aagtttttct ttagccatgg tctctaatgt ttgtttatcc aaaccttttt
    35401 tcacttcaag tttatccctt aattttccat tgacctgaag aacaaccgta gccgtatctt
    35461 ccaccgtcca tgcgggatca aaaacgggaa atggctcgta ggtgagtgac gttgtatgcc
    35521 caagacgcga ccataattcc tccgcaatat gcggagcaaa aggcgccaac aaaagcaaaa
    35581 acggttctat aacttttcgc ggccgcacgt ccgacggcgt aagttcattt acaaatatca
    35641 tcatttgagc aattgccgta ttaaaactaa gcgtctcgat atcatcttgc acttttttga
    35701 tcgttttatg caaaacgcgt aactgatccg gcgtcggttc gacatcctgc actttagacg
    35761 acagttggtc gcgctcatcc acaaaaagac gccatgtacg ctgaagaaaa cggtacacac
    35821 cctcgacacc gcgtgtactc cagggtttgg tctgtgtcaa cggccccata aacatttcgt
    35881 aaagccgcat ggcgtcggcg ccgtattgcg cgataacatc atcgggattg acgacattgc
    35941 ctcgggattt ggacattttt tgcgaatctt cacctaaaat gataccttga ttgacaagtt
    36001 tcataaaagg ctccggcgta gaaaccaatt tcaaatcgaa cagcacttta tgccagaagc
    36061 gtgcatataa aagatgcaac acggcgtgtt caccgccgcc gacatataaa tcaacaggca
    36121 accaatattt ttctttttcc gcatccacaa atgcttgatc gttatgggga tcacaataac
    36181 gaagataata ccagcaagaa ccggcccact gcggcatggt gttcgtttcg cgacgcgcgg
    36241 attttccatc catggttatg ttcacccaat cggtcatcgt cgcaagcggt gactcaccgg
    36301 taccggccgg cttatatgac tgtacttccg gaagattcaa cggtaaaacg gattcatcca
    36361 acggcatgat acggccatcc tcaaggtgaa ctatgggaaa aggttcaccc caatatctct
    36421 gacgcgagaa aagccagtcg cggagcttat agttaatggt gcgcttaccg atacctttat
    36481 tttcgatcca agctacgatc gtatcaaatg cggttttata attcattccg ttgattgata
    36541 cttccgcatt ggaagaattc acacagacgc tatccgtatt attatcgtac acttcttctt
    36601 gtacgttttt cgccgcttta acgacttcga cgatgggaag atcaaatttt tttgcaaatt
    36661 cccagtcgcg tgtgtcatgc gccggtacag ccataatcgc accggtacca tagttgatca
    36721 ggacgtagtc tgcaatccat accggtatcg gattaccgtt ggcaggattg atcgcatacg
    36781 caccggtcca caccccggtt ttatctttgg ccagctccgt ccgctccata tcgcttttca
    36841 aagcggcttg tttgcgataa gcttccaccg cattacgctg ttctgcgctt gttatttttt
    36901 caaccagcgg atgttccggt gcgagcacca tataggtcgc accaaaaatc gtatccggtc
    36961 gtgtcgtaaa cactttgata tccccgtcgt gatctttgat gcggaaatga atctcggcac
    37021 cttcgctgcg accgatccaa ttgcgttgca tttctttgat gctttcaggc caatccacta
    37081 aattaagatc ctccagcaaa cgatccgcat acgccgtaat tcggagcatc cattggcgca
    37141 tgggttttcg tacgacagta aaacctttat cgatttgttc cggtacttct tcattggcca
    37201 gtacggtacc gagttccgga caccagttga ccggtacttc tgcgatataa gcgaggtttt
    37261 tcttaaacaa ttgaagaaat atccactgcg tccacttgac ataatgcgga tcggtcgtat
    37321 tgacttcacg atcccaatcg tacgagaaac cgaacatttt gagctgacgg cgaaaggtat
    37381 ctatattttg tttggtggtg atggccgggt gcacaccggt ttttaccgca tactgctcgg
    37441 ccggcaatcc aaatgcatcc caacccatcg gatggagtac attgaaccct ttcatgcgtt
    37501 tgtatcgcgc aaggatatcg gtcgctgtat aaccctccgg atgacctaca tgcaatcccg
    37561 cgcctgacgg ataaggaaac atatccaaaa cgtaaaatcg tttttctttg ggaaacttgg
    37621 ggtcttccgt cacacggaaa gttttatgct gatcccaata agcttgccat ttggcttcga
    37681 ttttagtgaa gtcgtatttc gctgtgggtt gttcggaatt acctgtagat gttaccgaca
    37741 tactttcctc ttaaaagatc attgttttac gacggataat atgaaatggt caaaccaatt
    37801 tcaagccacc tatataaatc acatcgtcaa atatttgacg cactctatta acctgtgacc
    37861 tctatcacaa accgaataca cggttttcat gtataatcct attgaacgat ggttgtaatc
    37921 gttttttgaa agggatttta tgcagtacat gattatggca cttgcttttt ttatggttgt
    37981 tatggtgatg gcgatattct acggcaatgg tgatcgccgg gatcgttatt cagacggatt
    38041 gtaattctct atcgagcctg acgtttgtca ttgttctttg tatcgaagtt ggcttttttg
    38101 aatgatattc gcgaccgact tgagtttgcg gtttatcgga actatatttt caaaccattt
    38161 cgtcacatca ttcaccacga taaggaagcc aaccgtgtac aaagacatta ctccccccaa
    38221 agaaggtcaa aaaatttcaa tccaaaacgg tgtactccaa gtacccgata atccggtcat
    38281 accgtttatc gaaggtgatg gtaccggtcg tgacatttgg cgtgcatcgg tacgtgtgtt
    38341 tgatgccgct gtacaaaaag cttatggcgg taagaaaaaa attcattggt ttgaagtata
    38401 tgccggacaa aaatcatttg atcaatacaa tacttggctt cccgatgata ccatcactgc
    38461 ttgcaaagag tatctcgttt cgatcaaggg tcctctcacg acaccggtgg gcggaggcat
    38521 tcgcagtctc aatgtagcct tgcgccaaac actcgatcta tatgtctgtt tacgccccgt
    38581 gcgttatttt aacggtgttc cttctcctgt caaacggccc gaaaaagtgg atatggtgat
    38641 tttccgcgaa aatacggaag acatctacgc cggtatcgaa tggaaagaag gatctccgga
    38701 agctaaaaaa gtaatcgcct ggctacaaaa tgagatgggc gttaaaaaag tacgatttcc
    38761 tgaaacgagc ggtatcggta tcaaaccggt atcacgcgaa ggttccgaac gcttgattcg
    38821 ctccgccatc caatatgcca tcgcacaaaa acgcaagagc ctgacacttg tgcacaaagg
    38881 caatatcatg aaatacaccg aaggtgcatt tcgtgattgg ggttatgaat tagccaaacg
    38941 cgaattcgga ggtgtggaaa ttgacggcgg gccgtgggtc aaattgccta atggtgttat
    39001 catcaaagac gctatcgccg acatcacact acagcaagta ctgacgcgtc cggaagattt
    39061 tgatgtcatt gccaccatga acctcaacgg cgattatctg tcggatgcct tagccgcaca
    39121 agtaggcggc atcggtatag ctccgggcgg caatatcaat tatatcaccg gtcatgccgt
    39181 atttgaggct acacacggca cagcgcctaa atacgccgat ttagataaag tcaatcccgg
    39241 ttcggtgata ttgtccggcg aaatgatgtt tcgctatatg ggttggaatg aagttgccga
    39301 tcttttgatc aaaggacttg aaacttctat cgccaaaaaa acggtgacct atgattttgc
    39361 ccgcctgatg gatggcgcaa aagaaatcaa gtgcagcgaa ttcggtgatg cgatcatcgc
    39421 aaatatgtaa taccgaaaaa agtaaatatg aaacggcgag ctaaactcgc cgttttttgt
    39481 tttaatgagg actatagtga tttgtaaatc aaatcaaaat acctgcgaaa ataaaatgta
    39541 taaaccgctt ccgccttcgg aaatgccata atcagctcgt ataatcgtgc tatgccaata
    39601 aaatcgaagt cctccgccgt atccggcatg ccaatttttt agagtcatgg atggcccgtc
    39661 tttccgttta taaatctgcc ccgcatcggt gaatagtata accccaagca tcatattacg
    39721 gcgatccgac agacgtattc catgccaacg caattcggta ttaaccaaaa aacgaccttg
    39781 cccgcgtcgc cttgccgacg gcataccgcg caccgagcct tcaccaccca gcttcaattc
    39841 gtcataaaat ggtatctgac caaagacttg atctgccgct aaacgatatc cagccacgat
    39901 atttggtgtg atgggaatga aatgccgatg ttcataactc gcgagccacc cctgataatc
    39961 gcccgctcct cctattttat attccacaag tagctcttct aacatcccgc acgtggaatt
    40021 gatataatta tcgcgtgtat catatcgcat ggcagcgtcc aatttccaca atacaccacc
    40081 tttataaccc accggcttca gctccttaag gatattaccg gaatcagcat tgggcttgac
    40141 cgaagtatgg cgaatactca caccgccgcg caagcgccat ggcccattca aatcataaat
    40201 ccatgttagg gatgcgctcg gtgtagcttg acgaaacgtt cgttgtgatt tactcaaatg
    40261 caaactatcc accatacgat tggaaagttc tccgtagtaa ttggcaaact cgagcttatc
    40321 gtaagctaac tcaaaatcaa aacggtgacc tttaagaaaa ttcggataat ccaaagacaa
    40381 cacatgcgcc cattttccct ctgtcgtaat aaaatactgc gctttcaaag cacgacaata
    40441 cggcaccgta acgctgtcat aatcatacac gcacaggcgg agaccataac ccgcgccgtc
    40501 atcggatgta taattcacca agggtatagg cgtaaaacga cgccctttgg tgcgacgtga
    40561 tacatccacc ggtgtgtcac ctgagctgac ttgcagattc tcatacaaat tttgcccaca
    40621 gatcgtggtt gcgaaaaaac aactcaaaac aaaaatccgc ctgagtgaaa ttatatttgc
    40681 cgtttcaatc atcatctgtt ttaaattatc tgatgggcta tgcaatagct accggtatat
    40741 gacgcatacg cataatatga tacgtcattt tccataatcg aaaaaatccc ggaatcgttt
    40801 cagtaaaaat tacggttaaa gaaccccatt cgatttttaa attgacgtgt gtaaataaat
    40861 ttgtgacagc tacaaaagga gcaggcgccg aagtgagatt ttttgctaat gctaaactct
    40921 cggtgtacgg gacgacacga tcctgatcac tgtggccgat gatcatgtag gagtgaatta
    40981 agtgcgcata cgacgtaaga tcagccaatt ctaatttaga acgaaaatcg ttgggtaaag
    41041 ctgcgtacaa cacatcaaaa gccgagggat tctgattttc tataaatcga tacagtgatt
    41101 gatgcgtgct atccaatgaa agaaaatttt tcccggggtc atatccggca tccgttattt
    41161 tttgagcgat tatagattta aaaataacca gcgatttttc gttcccactt tccggtatat
    41221 tgcccaatag aaatttccat cggtcattat ttttagatac agagcgcatt tcttgaccgg
    41281 tatcggtcgt gtaatatcct gttaaagtat atttgagtgt ttttcgaata tcaaaataac
    41341 ccccaaaaat aacaccaaag cgtacttgat ctctgatcga agaatcagat aacgccataa
    41401 taaccggacc cgcaccataa ctgaatccca tcaaaccgat agacgacgag tcaacatccg
    41461 gtcgttgctt cactatacga aaagcttcca tggtgttgtc tgcatcttcc atacttatac
    41521 gaaaccgccg catcatagga aactcaggcg tcattacaat aaaaccggca cgcgccatac
    41581 gatgcgccat acgttgtaac cttcgatcgt tgtttccttt ttcagtaaac ccatgcgcca
    41641 tgataatcgc tgatttttta tgatttccgg gtggcgaata aatagaataa tacagcgttt
    41701 tttttgactc ccgatcgaga gaatcatgtg tgacaactat atcatccgtt acaacttgat
    41761 aaagttcgga ggcctccggc gtagccatat ctataataaa caaaaaagcc aacgcggcat
    41821 cacgcggttt ctcaaacaac aaggacacga ccgtaacaca gcaaataaca gcaaaccata
    41881 cgatgacacg ccgaaatgtt ttcaccggaa caatgatctt tcaaattcag acgaatacat
    41941 tttaagaatt acatttcatg ttgttttgaa atcatatgaa tatattactc agtagtaagg
    42001 tttgttcctt acatttattg gggacccggt ttaagatgtt ccttgagata ccgtgtcaac
    42061 aacgagtaaa gatgcatcgt ggttccttca ccttcatcaa tgtcatgcgt tcggttggga
    42121 taagccatca tgtcaaacgg tttgcctgcc gcgatcagcg cattaatcag catttccgta
    42181 ttctgataat gtacattgtc atcacccgta ccgtgcacta ataaaagttt tcctttcaga
    42241 ttttgtacat agctgatcgg cgaagctttt aagaaccatt ccaggttatc tttgggaaaa
    42301 cccatataac gctcggtata tatattatca tagtttcgct gatccgtcac gggtgaaaca
    42361 cacatgccga tgtgaaatac gtctggaaat ctaaacattg actgcaatgt cgaagcgccg
    42421 ccgccgctcc agccccagat acccacacgc gatttgtcaa tataaggcca cgtcgtcaca
    42481 cgacgcacac cttcggctaa atccatcgga gcaagccatc ccaattgttt atatatcgct
    42541 ttacgccaag ccctgcctcg cggcgccggt gtaccacgcg gatctaagct gatcacgata
    42601 tatccttgtt gggcgagcat ttgatgccat agataatcag tgtcacccca atggtcaact
    42661 accgtttgtt cggcgggttc gccatagaca aaaacaaaga gcggatattt tttcgtcgca
    42721 tcaaaggacg ccggcttgag catccacccg tctaacatca actcattacc ggcttcaatt
    42781 ttaaaaaact ccggcacatt tatttttaag gcagccaacc gttgccgcaa agcgaggttg
    42841 tcttccagag cacgtaatgt tttatgtttc ggaaggtcta caacttcgac tgtttgcggc
    42901 agttgcatag acgaatattt atgtatcgcg tattttgcat cgggcgaaat ctggtaatca
    42961 tgccaacctc cggtctccgt tgtaatgcgt tcggctttcc ctttaccgtc cattttcatg
    43021 cgatataaaa agcgttgcgt agcattttcg ggagatgcgg tataataaag catgttttct
    43081 ttttcttgtg cgagtataac tttgatcaca tcaaaattac ccggcgtaag aagctccgtt
    43141 tttttaccgt cgcgactggt cttatacaca tgccgccatc cgtcacgctc actaatccat
    43201 aaaaatcctt ttccgccatc cagtacttta aattcatcat ctatatcaat ccacgcgcta
    43261 tctttatcgg tggctatcgt ttgtacaatg ccggatttta catcgccgag catgatctgc
    43321 aaagtatttt gcaagcgatt gaggtgtcgc atgacgatgg cttctgatgt tcccgcccat
    43381 tccattcgag gaatataatt atcccgcggg tcgccgggcg tttgcatcca taccgttgta
    43441 ccgccgctgg cagaaactac accgacacga caggatgaat tcggatatcc ggctttggga
    43501 tactgcaccg gaatgacttt ggaataaact gaatccgtgt tgttaatcat ataaaacaca
    43561 ccgataccat cagcattcaa ttgccaatat gcaacagcac gactatcggg actccatcga
    43621 aatccgtctc ggcagccaaa ttcttcttca tagacccaat caaacgttcc gttgatgatg
    43681 acatcggaac cgtcgaaagt caattgtgtg attttcccgg ttaacaggtt ttctgtgtag
    43741 atattatttt ccctaacata cgccacttgc tgaccgtccg gggaaaattt agcaaacatc
    43801 agggtagatg ttttagcatc ggcaccaccg agcttatgta gttttcccgc cccaatgtct
    43861 agaacccaat aatcaccccg cgtattatat cgccaaactt tgcgtgtatt ggtaaaaatc
    43921 aataaccgtg aatcatcgga agaccagaaa taatcggata ttttgagagg acgggtttgc
    43981 ccctgcggta ttagcttttc agcgccgatc aatacggtac gcgtcccggt agccgcatca
    44041 tacttgatga tatctttgcc ttccactcga tccgacactt caagcgtcgt ataacctttg
    44101 ccgtttttta accaccttgc cggaccaaaa aaacgtggct gcaattcatc ggaatcaaaa
    44161 attcgttcca acgataacat cgtgctgtcc tgcgaaacca atggccattg cagaaacaaa
    44221 aacatgctca gaatgaatgt tttcatgccg tcaccttttg ttgagttgaa ggctgttatg
    44281 atcaattaaa ttcaaggttt ggctgtcggt gcatgcgacg tctgaagaat gatgatgcct
    44341 atgataatcg tgactaaacc gatacctgtt tgccagttca tcggttgatt gcctttgaat
    44401 aatcccatca cagtgcctgc gatcggtgaa atcgaaaacg ccaaagccaa cgtaactccg
    44461 aggttttccg tcgtggataa ggatttgtaa aaactccaca tgccaagact tccggctact
    44521 acgccgccac cgatgatgat cataacccac gccgtcgtat cgtttacatt ggcaatctgt
    44581 ttccatgccg gtatggataa taacgctaat aaaatgaacg ctactccggt gcgcaaagta
    44641 atccctgcaa tcggtggtat tcccatcgca cgcaatcctg atttttcaaa ataccctccc
    44701 agcccccatg ctaatcccgc taatacggca aataatacat agtcacgcat aaaactcctt
    44761 aaacaattta aacgaatcgt attccaatat gacacgcttc gttagaaaaa agaatgattt
    44821 ttgacagcag gcatgcagct cgatatgcaa cagctatagc aatcggtagg aagtaaaaaa
    44881 aattacaaca aaatgataag gtctatcgca gtacaaaaaa atcgttatgt ttcaggtcgt
    44941 tgcgatatgg gataccaatt tttttgcgcc gatatcacga agttttttgg aatgaccgtt
    45001 accgcctttt tcagcgagtt ctttttcttc caaagccttc aggattttat ccggtgtaaa
    45061 tggcgtcgta tccagacgta caccgatggc attgtagatc gcattggcaa tcgccggtat
    45121 cgccgcgtgg agagggcctt caccggcttc tttggcgccg aacgggcctt cgggatcaat
    45181 agactccacg ataatggatt cgatttcggg cgtatccaat atcgtcggca ctttatattc
    45241 cagaatactg gggttgcgat gcaatccgtt tttatgaaaa atatgttctt caaaaagcgc
    45301 ctcaccgaca cccatataaa cactgccttc gatctgacct tcaacgatca cgggattgag
    45361 cgctcgcccg caatcatggg ctgaccatat tttttctacc gtcaccgcac cggtttctac
    45421 atccacggac acttcagcga tatgcgtaga aaaagtgtat gagggtgatg gacctgcacc
    45481 ggcgcctttg taagatccgc cgattttggg cggcttgtag ctgccggtcg caccgatcgt
    45541 accataggct gattccgcca tgtttgcggc ttcgacgaaa tccataaatt tattcggatc
    45601 cgattttgaa aaaatcaatc gtttaccgtt atcaagatca gttactggta caccgagttt
    45661 acggctcgct atttcaaaga tcatttcttt aatgcgttcc gccgcttgta tgcacgcatt
    45721 gcccgccata aaagtgacac ggctcgaata actgcccaaa tccaccggcg tcagatgggt
    45781 atcggtttga caaacgtgaa catccgatat ttcgacaccc agaacttccg ctgtaatata
    45841 agctaacatc gaatccgaac cctggcctat atccgacgct ccgcaaaata ccgtcacacc
    45901 gccgccacga tccactttga tctgcacgcc ggaatgcggc atatcattcc aaatgatgga
    45961 ttttcccgct ccgctgatat atgtgctggc cgcaataccg atacctttgc gaataacttt
    46021 actgttttgt tttttatacg acgcgtactt tttatcaaac tccgaacgcg aacgcgtaga
    46081 agtgaggcat tctttgatac cgcatgaggt gacacgaaac gaattgatcg tcatcgtatt
    46141 gggttcgata ctatttttga gacgcatttc caccggatca agaccgagtt tttcggctat
    46201 tttatctaat tggcattcca tcgcaaaacg aggctgcacg gcaccatgcc cgcgtttggg
    46261 cccgccggcc ggtttattgg tataaatacg atacgcatca aatttgtacc gaggcacttt
    46321 gtaaggcaac gtcagaaata cgccggaata atacgtagtc acaagaccat aacttccgaa
    46381 cgatccgccg tctagaaatg tacgcatgtg gatcgcggtg atcgaaccgt cctttttgac
    46441 gccggtttta agtatcatct tcatcggttg gcgaccccga tgcgcgtaaa atacttcttc
    46501 gcgggtatat aaaaatttca cgggacgacc ggtttttttg gaaagccatg ccgcacaaaa
    46561 ttccaatgca aacggttcgg acttcccacc ataacctgca ccgatatgcg gcttgatgac
    46621 acgaatatgc gaacgcttca tttgaagcac tttggctaag gctacatgca gataatgcgg
    46681 tgtctgcgtc gaagaccata tagtcagttt accgtcgctt ccgtattgag ccagagcgca
    46741 atgcggttcc aaaggtaagt gcgtattgcc ttcaaagaaa tattcgtttt ccataaccaa
    46801 atcggcttct ttgaaaccct cttccacatc accgaactct tgatgcactt ccttcattac
    46861 attggcttgt ttggtgtgcg ggtgtatttt aggaagatct tcacgaaaac catcttcgat
    46921 cgtaagacac ggtttcaaaa cttcatattc gacttcgata agttcgcacg ctttttccgc
    46981 cgtctcttca tccagtgccg ctacggccgc cacaccgtca ccgatatagc agactttatc
    47041 aacagccagt acggtttcat cctgcgaagc aggaagaacg ccgtattttt ccgtaagttc
    47101 cgtcccgatc attaccgctt tcacgccggg taatgcctcc gccttagaag tatcaatttt
    47161 tttgatcagg gcatgcggat gcgggctgcg taaaattttt ccgatgagca tattgggaag
    47221 catcagatca tcagtataca gagcggatcc gttggccttc tccaatcctt caattttcgg
    47281 attgggtttg ccgataatag aaaattgatt ttttttcgga ttaggcttta tcattatgat
    47341 ttctttcggt aatcaatcaa ataattttta aatttttata acgcaaatcg cgattaaacg
    47401 gctttttctt tttgcattat cgccgcggcc tctttcatgg cggttatgat cttagtataa
    47461 cccgtacaac ggcaaatatt gccggataag gcctctttga tttcctcatc gcttggatta
    47521 ggattttttt ccaaaagcca cttggccaca accataaaac cgggcgtaca atatccgcac
    47581 tgggatgcac cttgattatt ccacgccgtc tgtagcggat gcgcttctgt tcctttagcg
    47641 attccttcga cggtgatgat ttctttgccg acgcattcta ccgcaagtgt gatgcaggcc
    47701 tggataggga cgttatcaat taacaccgtg cacgcgccgc agtcgcccat atcacaacct
    47761 tgtttggaac cggtaagatg gcaatcgtag cgcagcgctt caagcaaagt gcgattggat
    47821 ttgacggcaa tttcgtaaag gtcgccattg accttaagtg tgatcaacga tttcatgatt
    47881 ctccgactat actatactat aagaaagcat tatgacaggt aggcaaaact atcgaatgga
    47941 aggtagataa tcaagatttg actgcaatga tggtcaaccg atcgccaaag cgtcccgaca
    48001 acttcgttag agggtaattc aaacattgaa aaacatactt ggctgtattt tgaacaacgc
    48061 tgctgcgcaa accaatttca ccgggtttca taatcaccgg tttcatatct attttagaaa
    48121 aaccgtggtt aagcaacaaa cgtcgaaccg atgaaggtcg gtattcaaaa agatgatacg
    48181 gcggcatatg tatccgcgtc cgtttgccgg ctaacgaaaa taaggtcatg ccaaatcgcg
    48241 aaaaaagtgt attggtttgc gtaggaataa cgatcaccag tgttccaccc ggtttcagcc
    48301 attgatacgc ctgcttgagg agtaaaacag gatccgtcaa atgttcaaac acatccaaca
    48361 aatagataag atcaaatttc tccgacgaaa actgcgcttg atgaacggcc atcgtatcta
    48421 catccaagcg atacacatcc cgcgcatgac gggctgcatc ttcggatatc tcaatccctt
    48481 tgacatgatg ccccgcatca tgaaatgctt taagcgtata acctgtcgca caaccaattt
    48541 ctaaaacgga tgactcaggt tgaaggtgaa cctgctcagt ccaatccggc agaacaggat
    48601 ccgtcttttt attaaaggct gatgcatctt catgcccgca tctaaaatcc gattcgaaat
    48661 aatccttact gtacatacgt acaatcgttt tttcgtcagg ttgaggcgat acaaatccaa
    48721 tgcggcatga tatacactgc aaaagatcga aacattggga ttcaaattcg taatacatcg
    48781 gccatttttg atgtgtactg tggccacaaa tcggacaaat attcattttt agaaacggat
    48841 agataataat tgtatttggt tgtctgtaga ataaaaatta tataacgaaa taaaaagaga
    48901 aacttgttgt aactaaaaac attgtttaat cgtataagaa tctaattttc tgaattatta
    48961 tcatacagtt cttaatctat gatttggacc atcatataca cgctcatatt cggccaagga
    49021 atttttcttg ccatcgttct tttttttaac aaaaaaggga gcgttctggc aaataaaagc
    49081 cttgcgctcc tgatgcttat tttttctagt gccgtattgg aacaattgat tcgcatctcc
    49141 ggtctttttt ccgccctgcc ccatatgctc ttcatttcgg tccctttttg gtatgcactg
    49201 gctccggctt attatcttta tgccaaaaat cttgtcgaga agcaaaatca attcgttatc
    49261 aaagacgctt ggcatgcatt accttttttg ggagtgattc ttctcttatc cccatactat
    49321 ttgtgggatg tacaaaagaa aatcgcatac ataattgatc cgtcacagtt caatgatttc
    49381 gccttagaga gtattattct aaatgtcgta atggttttgc aaaatgtcat gtacctcact
    49441 atggccattc gtttgctgtc acaaaatgaa gcgtattcta aactcaatcg cattatccga
    49501 ttcttattta gcattatgat gggatatgtc gttttcaatt ttataaatgc tatttacttt
    49561 cttttcaccg gagaaacgct tgtccattgg ggtaattggt caataccgat ttttgcaacg
    49621 attatttaca ctattgcatt cctttcattc gtttctccgg aaacgatttt ttttacttta
    49681 tggccgagaa acaataacag cctttctaaa gagagaaagc atgaagtttt aaatcaatta
    49741 tacaaactga tggaacagga caaaatatat cgagaacccg acctgcgtta taccgaagtg
    49801 gcggcgcgcc ttggtatcag cgcacgatgt ttgtctgcac tgctaagcga agacatcggg
    49861 atgacgttta acgtgtttgt caatacgtat cgtgtaaaag atgcccaaaa ccaactcaga
    49921 gaacgatact gcgaagaaac tctgctggct atagctttag attcagggtt ctcaggtaaa
    49981 agctctttta atcgcatatt caaagagcaa accggtttaa caccatctga attcgtttac
    50041 caatccaatt agctttctga tcaatttgtg tttatgtaaa taaaaaacct cgatttcagt
    50101 cgaggttttt tatttcacaa gaagacatta cggcaatgcc ggtggatcgc tgaaagccgg
    50161 agggccggag gatccgcttt cttttttatt caacatcata taagccgcca caccgccgcc
    50221 aacagcaagt aaactgccca tcacccacca tttggtatta ctcgcaacgc ttactttcaa
    50281 aggcggaagc ccggcaaact gtcgtgcgac attgcgaatg acctgtgtca agccttcggt
    50341 aggaccatta taatcctgct gcgaagccgt gatgacgcga ccggatacaa catcaatcag
    50401 tcgtacgtcc accgtatatg aacgaccaac acgaccgatg gaacccacta cgatttgttc
    50461 gacgctgagc agcttcccgg ctttagccgc attggcttcc gtcatatcag acatcgcaaa
    50521 atcttgttcc ttaagcaaag catccatctg agaacgttcg atgatatcaa atttctttag
    50581 tccttgcatc tcgccacgta aacggtcgga aagcgattgc gcttccgatt tgagaatacc
    50641 tccggtaacc tgtagatcaa gaactgccac taatggcctg gtgccctgtt gtgcgtgtat
    50701 agaatgcgaa aacagcaatg ccgcctgaat attccaaaaa gcagccgata acaagacagc
    50761 tatgattttt ttaaacctct tcataaatct atccttaatt tattttattt aatcaaagtc
    50821 attttacggg tctgtgaaaa caccttgtta cttcccagcc cggttgcttc taaacgatac
    50881 acatatacac cgctggccac gcgttgaccg aaatcattgg tcccatccca taacaacgca
    50941 tgcgatgccg caggcatcac ttgtttgacc aatgtacgca cctgctgacc caacatattg
    51001 tagatcgtaa tttttactga ggacgcctca ggcaaaccaa atcgtaaagt ggtgctcgga
    51061 ttaaatggat taggataatt ttgagccagt gaaaattgtg atgggataat ctcagattgt
    51121 gcttttgcaa taaaagcctc cgtacctatc agtagtttat acatctttgg ttttccgttt
    51181 tgcaccatta ggttcagagt ttcacctgtt tgtaccatac cggtagtcac ctcgaccata
    51241 cgtaccgaaa aaccttcagg caaatttgtc gctgaatagg ataatgatac cgtgcgacga
    51301 tcaacgcccg tagctacctc aaacgtatat acatgacctt ccggagaaac ggcgcgcata
    51361 tccgtttgca atttccgatc cgcttgcaac cagtgaagcg atacctcatc tttcatcagc
    51421 atagggagcg ctcttttatc acgaccatca gcaccgtctt gcgcatcttc cgcagttcct
    51481 gcttcaaaca ctatatcttc gccgtcatca tgtaatgtta gtagaaaacg ataaccgctt
    51541 ttatcaaccg ttgttcgaat ctcttttttc tgcaaagccg atgcattgtc cgaattgatc
    51601 gcgggataat tgattgtaaa agtgccgttt tcttcgggag aataatacat gtatcctttc
    51661 cacggctcta atttgagatt gtacacatcc acttcatcca catatttgaa acgaccattt
    51721 tgtaaaacta caatattggt taattccaca ttgtcgttaa agttagcaac gtcagaccag
    51781 ttaatactaa acgcatacgg attcgcgatc atgttccatc cgggttggat cgttacatgg
    51841 gcatcctctt tttttactgt tttccctgct ccgctattga tggttttagg tttacgagaa
    51901 accaataaat aggattttcc tgactcgata tttccaaatg taatatcagg gtctgaagaa
    51961 gagataaatt tagaaaggtc gttatcatag cgatatactt gccaatcacc tttgacaccg
    52021 gcttcgccca tattacttgg agtaaatgca ccgcccactg ttttattggt tagattgagc
    52081 ggaaaggaca tgagctgaaa agccttttta tcagtaccgc ccggcaaaga aacataaccg
    52141 ctactactta atccaggatt aggaatcgtc actgagacaa cctgtggttc aaattctccg
    52201 ttttcaggat attggatcgg tgatacactt gaagaagatg taaattctac gtaaaactct
    52261 agcccgtttt gtgtaatatc ttcctgaggt attgttgttg taaaccggct tgaattgccg
    52321 ctttgtgtta cggtaggcgt caatgttttg ttcttatacc ctgacgaacc ttctccattt
    52381 ttacgataac gcagcaaacc cgtgacttcc aacgcatcaa tctgacttag acttacttcg
    52441 atggttaatg cctgattcag tgaagcgctc aggttatcaa tgttacttac ttgaatcgag
    52501 gagccgacac ctttcactac gatacgatgt tttgggttag gcgtattatt cgccttaaac
    52561 gccgtgcttt ctatgatcaa agtatctgta taggatttac cttcggttgg ttcaaatttt
    52621 aagataacag gaagcaccct acccggagca actataaatg aagtactatc actttcgtta
    52681 atcgattgaa tggcgaaaac atcgtttttt gacaaatata tatcttcgat atacatgtca
    52741 tcttcaccgg aattaaacaa ctgaaagtta gttgtttttg acgcattgac taacgcacct
    52801 ttgacatcca aagtatcatt tttcagattc gttccgattt gtgaggtttt tcctataccg
    52861 accagacgtg tcgtggctat aatcgtactg tccgatccat tggacgctcg aacggtataa
    52921 taagatttta gtttggccgt tttcaaccct ttagatacgg gtttgaattt aataggaaaa
    52981 cttgtctcgg atcggatatc taagaacaat atgtctccat cggcaaaatt tttatctgca
    53041 atggaaaacg aattttccgc atcatccgtg atttcaaacc gttcaatgta agaaaaagcc
    53101 gtgccggtat tggcatactt gagctcagca atgctggaat ccaacaatgc agaggtgctt
    53161 gaaaatatga ctagagtatc tatgctaata ctagctgttt gtgtcatcgt ccaaatacca
    53221 ttattagccg ccgcataaat aacaacagca ttttcttgaa aacgatattc agttaaaaca
    53281 ttttggatat taaattttga tagatcaggc gttgttatct cagtccatcg gggttctttt
    53341 ccaaaccctg ctttatataa cggtctagta tattccgctc cattcgctcc aaagtcagac
    53401 cgaaaacgta tcatataata cccatcgacg ccggcgtgaa tatcgtgaat ttcggattgt
    53461 ccgaattcat ctcttggaaa tgagccggaa atttcttcga tcgaaaaatc agaactcata
    53521 tcatcacgat gaccgatgaa taaacggtca ccaccgggtt cttttgccca aataaaagct
    53581 tccgtctctc taaattcgat ttttttgatt gtgaatgaac cgtcaaatag tttaatggtt
    53641 ttccaagatc cgaaagttcc atttgcaaag tatgttgttg ctatgcctgt agacgcggca
    53701 acgtagatgt caaccgtatc gcgctgatag cttctaaacg aaaatgaaaa cgatgtgatt
    53761 ggtgatgtaa gagcaatatc cggcagtgta ggaatgtatg aaataacatt gtttgttgaa
    53821 aaatcatacc gcgcaatgaa aaaaccactt gaagttccac ctgttgaatt ttgagttatt
    53881 ccgaattcgt aattatcgta aagaccggat gaaaaaactt cactaccgat gactgcaatc
    53941 accgaatccg ttcccgaagg aaaggttata gcgccaagaa cggcgccagc actttgtaag
    54001 taggagcccc gttttgtcac aaaaaaacca ttaccgccga taatttcatc ccccggcggt
    54061 aacatgattc ccgtttcata aaaatcttga ccataattgg atgtgtatat caaatcccga
    54121 gtttgagtct cagcccacac ataattattt tgagaataat gaaccttttt tatttcttgt
    54181 gcagtaaatc ccaatgactg attgacatta gctacatatc cgtatggtgt tatggataaa
    54241 cgcgccggtt ttttgctata attacaataa aaaatggacg tgtcttgcgg cgatcgtact
    54301 acagttcctc cacgaaccaa tgatagatta ttattgagac catccttcat acgataccaa
    54361 gataacccgc cattgtttgt tttcagaata ccattttgtg ttgcaacaat gtgctcatta
    54421 ttgcctttag aacgatgaag ccaggcgtat tggccgattt tgaatccccc ttggtacatc
    54481 gccacatcat cggagaaaac aatcgcccac gttgcaccat aatctaaact tcgcattatt
    54541 ttggtactat cctcattacc tattaataca tatacggccg caatgtttgt gttttccaca
    54601 tgcactttac cgattcgcca tccggtttcc atgtattggt acgtatgaac attggaccag
    54661 gcattgcgtg tgccttgaaa aatatcgcga tcgaaagaat tatcgtggcc ggctgcatac
    54721 attacattcg tcggatcatg cggatcatat gcaaacgaat gaaaaccatc cgggaagtat
    54781 ggaccactac caacaaaact ctttccgcca ttaattgaag gaattgaacg gtttaccgtt
    54841 actaagtgca tttgcgtgtt gtttgccgga ttaataacga aatctttgat ttcctcatta
    54901 tacagattta atgcatagtt cccggtccaa gtaagacctg cgtctttaga atagaataca
    54961 tgattaaagt tatttctgtc atgtactccg acaaaaatgt tattaccatt tgatgaatcc
    55021 actactaatt ttgttattgt cccgtcaatg gcgctcaaaa acgaccacgt ctgacctgaa
    55081 tttgtagagc ggtatatttg accattacgg gttccgatat acaaattatt aggtttcttt
    55141 tggtctatgg ccatagtttc gtattctcct ccatagggtc ctagattgat ccagtcactc
    55201 tgtgcttgta ttggtacaat acaacacatc aaaagcaaac gtatccaacg tgccataaat
    55261 cctctcctcg ccatgtgaaa taaaaaagaa atgtctcttc ttctaaaggt atgccaagtg
    55321 ttaatactgc atgagaatga tttgtaaacg acaaattaat agaaatgacg ccgataggca
    55381 atgagaaagg tcaaaaaaaa accgcttgac tcttttgcca agcggttttt tacaaaactt
    55441 ctaaagtaca atcttagtat ttgatcttaa cttctttttc tttgccgtca ttatctctaa
    55501 atttgataat gatttcttca agatctttgt cttcaggaat ttcattttcc ttggttgtat
    55561 ccacgttcaa attaccggac ttgtccgaat tagcagtatt accggccgaa acgtcttgct
    55621 ttttattcga tgccttattc agaacttcga tcaaaccttc gatgccgata acttttgtac
    55681 cgttggtatt ttctatcacc caaaactccg taccttttac cgaagcgact gaggtcggtg
    55741 tggcaacttg gaaagaacct ttttgtttgg atactttcac gaaaagttcg ccgacgtcca
    55801 tcacgatgtt tttcgaaatg gatttacctt catattgacc tttaacttcg agattggaat
    55861 tgggcttaag cttgataata ctcttatcat caagaaacat cacggcagca aaaccatcat
    55921 ctttggttac cactttatca cccgaaaaaa gtcttgatcc cttagtgaca tcaacccatt
    55981 tgttaccttg tttggcttgc gctttaccgg tggacttgac gacgatcgca accgctttgc
    56041 catcattggc ttcggccaaa tacaacgtcg ccgtgatgac cgtggcaaca caggccaaaa
    56101 gcgccgtcat tttgatcatg aatgatttca tatattttta ctccaaattc tgttttatag
    56161 atttgctttt aagtttacgg tttaatttca aattttacag cttcaggcag gttttgtacg
    56221 gctgtgcgga gatcatcgat ggtagctgta ttacctttga tactaatgga aaccgcttta
    56281 aaacctttaa gttgatcttt gaaattaacc gctcctttag ccgcatcatt caaaaggcca
    56341 aggatttctt ccgtattgag gccggctgtt gatgatatag tttcttttac tgtaaaggcc
    56401 cgaatcggac tgtctacatt gcgggattta ccggtgaaat tatcttgaat aacagctgtc
    56461 acttggaata cataatcacc cggctctaaa cccggattac aattaagatt gttttctttg
    56521 gaggtcgagg gaccgcctga aggcaggcta tatgaaaaca cattatccaa ttcgatcact
    56581 gcacgttgta ccacggattt gatggcacct tcatccgatt gcgaaccttt tttacgagca
    56641 atagtcaata aaaatttcgt cgcaccttgc gcagaccatg caaattgagg aagtccgtct
    56701 atagattcaa agccaaaaga cggagaaatg atctcaacgg gactatttcc gagaacttga
    56761 atctgaagcg accccatata atccaaacca tcataattat taacattggg aagacccgct
    56821 ttaatatcaa atgtgaaaag accgctggga aagcctcctt tgatctgctt cataaagtca
    56881 tcgctaatac taattgattg cctgtttgat gctttgatag aaccaaaact gccgttcaat
    56941 atctgcggaa cgcctacaaa aatcggcacg ccggagggaa tagttagagt caccaaatat
    57001 ttcttattaa atatcgaccc ctgagacgtt tttaagtcta tacccattct tattattttg
    57061 acagatccgc tattattctc aaatctcaac ccgaaattat taaatgaaga taagttggca
    57121 tcggaaccgc caatacccaa aaggcttaaa ggaaacgaag taaaggtgaa cggtgtcaat
    57181 ggttcaggag taattgttac cccattcgtt gttccttgcg caacaaccgt agtgaacgaa
    57241 aataccgcaa cgatcagtga caacccgacc atttggcgga accgacttaa tacagcctta
    57301 agttccatac cgtttctcct tataatattg tgatgaaaaa gatattcttt tggcattttt
    57361 tgagtaaatt aagtttgaat taattataat gctgtgatat gtgtcacacg taacctagat
    57421 aaaatataaa cttaaccgat ggtaatttca agacttggga aagtcttttt tagatttcgc
    57481 agttttactc ccgtcccatt atacattttt cttcttatta cgggcaccta tgacgccata
    57541 ccatttatta caggtacgtt actgatcgtt atcggtgaat cgttacgctt tatgactgtg
    57601 ggatatatcg gacaggaatc ccgcgtttcc ggcgttacgg cccctatttt aataaaatcc
    57661 ggaccatacg cgtatgtgag aaatccgatc tatacgggta atattttaat atatttaggt
    57721 tttaccgcct tgtcaaactc tttcttgata ctcgcaatac cttgcgtatt tatttttttt
    57781 ggaatgatct attactgtat cgttatttat gaagaaacat atctgcatga aaaattcagc
    57841 tcggattatg aattatactc ccaattgaca ccgcggttta ttccaaaata ccgtacggta
    57901 catgaagaca ttcatcgccc ccaattccgt tttgacaaac acaccgcaat caaaagcgaa
    57961 cagccaacgt tcgtagcgct ggctgtctgt tataatctat tggttctaaa attttttcta
    58021 ttttaaacct tttaatcttc aacgtttgcg gctttgagta gcaatccccg atccacaatc
    58081 ttgatgcgtt ttccttccaa ttcaatcaaa ccatcactgc gaaattccga tagcagccga
    58141 atggttgtct ccgtggccgt tccgacgatg ttggctattt cttcacgcga taacgatgtc
    58201 cgtagtgttt taccgtcatt ttccgttccc ccgaattctt caagcataag taatgtttcg
    58261 gcgaggcgtt cgcgtacggt attctgcgcc aagttgacga cgcgatcttc ggatgacttg
    58321 agatcatgcg agagcagacg aatcatcttc atcgaaaatg tcgtattgga atccaggaga
    58381 ttcaaaaaca cattacgcgg tatataacaa ataatcgcgt cttcgataac agccgccgaa
    58441 ctcgaataca aatcgccgct aatgagcgag ctgtatccca aaacatcgcc ttctttggct
    58501 aatcgaatga tttgttcttt accgtccgca ttgatgcggt acgtttttat cttaccctta
    58561 ttgacgcaaa acaaacctgt cggcttattg ccttcataaa aaagcacttg gcctttttta
    58621 taaatattgc cgcatttatt ttcgttcagc gacttgacat cgctgtcttt gagatcacca
    58681 aaaacggaat gaacccgtga atggcattga tcgcatagcg gaagttcgaa ttttttattc
    58741 atatatcaag acccttgaag cgcgtacgtt atggttctac tacaaacaaa ggttgccctt
    58801 tttctacatt ttttccgcct tgcacatgaa attttttaat cttcccggcc atcggcgctt
    58861 tgatttcatt ttccattttc atcgcttcaa gtatcagaac accttgccct tgattgaccg
    58921 tgtcgccttc ttgtaaaagt aacttgctga ccaatccggg gattggcgct tttacttcac
    58981 cgaccgcttt agctccggag ccggctatta atgaacgcat caaaaatgtg cgttcatctt
    59041 cgacattaaa ctcaaaacga tgacgccggg tatatacggc gtgtttgtgc atttttttat
    59101 caataatcac acgatacgat ttgtcattga ccagcatcga atgaaccgat ccctggccta
    59161 cggtgtgaaa atctacatcc aattcttcta tatcaggaga tttgcctacg cgcactttga
    59221 agcttgcgcc ttgcggtacg acttcgatgt cgtactcttt ttgttcggtg gttactttgt
    59281 atttcatgag cgtactcact tatcttagac agtttaatct aactcacatt atttaaaata
    59341 acaacaaaaa tagaagctta gctgttcatg atcgcgccgt tattgatatc caacgtctga
    59401 ccggttatac tgcgttgctt tagcagataa tgagttagat cagctatctc ttccggttcg
    59461 ctcattttcc gcaaaggcac cgattccata gccatccgat aaaattcttc cgtcgttaca
    59521 ccgatatttt tggctatacc ttccatacca tcctgtgcca tttttgtatt gacccagccc
    59581 gggcagaccg cgttgaccaa aattttctca ggtgcaagct caaccgccca actccgcatc
    59641 agaccgagca aacctgcttt actcgcgcaa taggccgaat accttgccac gccaagtctt
    59701 gctaaaatag acgaaacaac aacaatatga cggtatgtat tcggcgattt tcttacgatt
    59761 ggtagaaaat tattaacaaa atgatatgta cctgtcagat tggttgcaat gatcgcatcc
    59821 cagcgatctt cgtcgccata ttgattttct cctccgatac cggcattgga aataatacca
    59881 tctagatacg gtatagccag ttgggccgcc gcttttttta tcgtgcccgg attatgtata
    59941 tccatcacca atatcgaatg attgcgcgta ttggagagtt gatgcatggc ttcgcttaaa
    60001 acatcctgcc ggcgaccaca aagtattaca ctgtgatcgg cacgttcggc taattttttg
    60061 gctattgcaa gaccgatacc cgttccggct ccggatacaa gataatattt ttggcgcatc
    60121 gttggttccc gttatgtgat acgatggatt atttttattt tgtccaacgg aattcggcga
    60181 tgatcgtttt gctgtttaaa ttccgtcggc gttaagcccg ttatttgttt aaactgtttg
    60241 gataaatgag gcacgctgct gtatccgagt ttgtatgaaa tctcactgag cgtccgatcg
    60301 tcgtacgcta gccattcttt cactttttca atcttttgca aaatcaaaaa gcgttctatg
    60361 gtaatccctt ctacagatga gaaaaggccg gaaagatatg aataatcacg accgatcgta
    60421 cgcgataaga gtacggataa tttaatattt ttattttcga catcgttttt ttgaacatag
    60481 atcacgattt gatttttaat ttcctctacg atttttgcac gtttgtcctc aagaagttca
    60541 aaaccatttt gagtcaatac cgttcgtagt ttgttttgca tcggcaaagt tagattaccc
    60601 tcaacgatcg ctttacccaa ttcgacatga cgcacattta cgccgatatt aaccaattct
    60661 tcacgaacca cgcggatgca tcggtcgcaa accatatttt ttatggctaa agtagtaact
    60721 ttaattttta tgcccgagcc catcgcacca tctcgcgaaa tgatattttt ttcccatgca
    60781 tccgcaaact tatttcaaat aaacgaccgg ccatacgaaa caccaagact acaccgacaa
    60841 aaagtgttat gaatgtcaac cctatttccc accacggcgg attggataca ataatacgaa
    60901 gaatcatcat cgtcggcgtt agaaatggaa cgtatgaaag tattcgtatc gcgatatcgg
    60961 atggcacttc aataacccaa aacgccaatg ctaccggtac cacacccaac atcgaaatga
    61021 ccgcttgtaa aggctgtata tcctgatctg aagcgaaaat agctccgatc aaagcattga
    61081 ttcctgcaaa aaacatatag cccatcacaa aatagagaat aaaaaaaagt agcagctccg
    61141 gagaaagtgc ggcgatttct ttacgtgatc ccaacataac ggccaatgtt gtccatactg
    61201 caatttgcaa caacccgact agcccgagac cgatgacttt acccgccata atttctctcg
    61261 gcgaggcgct ggcgacaagc acttcaatga cacggttgtt tttttcctcc gatagccccc
    61321 gcataaatga tccggccgaa ttggcaattg caaaatacaa catcatcacc aaaataatcc
    61381 cggtcatgta tttttgtaac gaagcggcat gatcactgcc tagcttatat gtgtctactt
    61441 gtaacggagc ggtgatgttt tgaatttctt cgtgtgtaaa atttttacca cgcatacgat
    61501 attccatcag cagaccggca accgcttgtt ttaacaattc tatctgtacg gcattctcgg
    61561 tacggtagtc ggtatatatt tctatctttc gctgagtgaa catatccgca gttaaaacca
    61621 gcaccgcatc aattccgtct tccttggcaa ggcgtttacc ctctgctaat gcctcttcca
    61681 cattggacga agccaaaggt acgagctgaa tttcgctata cgtcaaatct tgcagcttcg
    61741 atgatagatc ttcgcccaaa cgccgggtgg catcaaatac gccgacagaa aaaatacgat
    61801 tgccgttaga attggcaaaa aacaccggta gcaatgtaaa ggcaagaata atggcaggaa
    61861 taaaaatgac tgaaaaaaga aaaccctttg aaatcacacg ctgagtaaat tcccattgta
    61921 tgatagtacg tattttcgca ttaactaaca tatttactgt atggcattct attgcaaaga
    61981 catattccgt acatatataa tatacaaaaa acttagcttt aattgcgata gccaaaatgt
    62041 aaatttacag tcatgggttt ggattctcaa gcatacatca ttaaacagta tttccggaat
    62101 tcgtttaaca ttagtttttt ggtgtatata gaatgctaag ctttcacata taatttttcc
    62161 attaagaaac tcaaatgtta tcgcaacttt atttgaagac actctttttt ggaaaatcat
    62221 tgaatactag aacttttttt tcgtgaattg aaacataacc atgtgattgt atccctgact
    62281 tcgattcaaa acgcgattta cgttcatcgc atcatttcaa gttgaataac atgaaatttg
    62341 aatttttgtg atcgttatat tcaacaatct gtgaaacttt ttttctcttt tgacgttttg
    62401 acattacttt tccgatttca aactcacaat ctcttctttg aggctaatat ggcgaaaaga
    62461 gttagctggt tttttctact tgccatagtg actatggttt tatgttcatg ccgtacgcta
    62521 tatcacactg aaactattgt tttttacaac aaccagattc ggttttccgg aaccttttac
    62581 aagccgttga cctcagctcc ttatccggta atagtgatcg cacatggatc tggcacggct
    62641 gatagaaact ccttttttta taaaccttat ggtgagtatt tctcaaaaca cgggattgct
    62701 gtatttatat ttgataaacg cggggtaggc gattcggaag gagtttacga cgatagtcct
    62761 gattttcaac ttcttgcttc cgatctgaaa gcagcatttc agtcattatc cagtcggaat
    62821 gatattgaca agaataaaat tgggattgtg gggatcagtc aagcggcctg ggttcttccg
    62881 attacagctc aaaatcttga tggtttgtcg tttgttgttt gtacttcatg cccgtcagta
    62941 cctccttatg aatcggatct ctttcaaaaa gggcgtgaac taatcgaggc tggttactca
    63001 cagaacgata ttgacgatat cttaaactat aatcgtgctg ttacagaata tgttgcaaca
    63061 tttgtagggc gggaaaaagt cttgcagatg aagcatcgtt tcaaggatcg gcaatggttc
    63121 agagatttca attataatcc tgatctgtca cccgaggaca ctttgaagca accacagtac
    63181 gatcattatc gcaatagtac ttttgatccc atgccgtttt ggaatcaagt aaaaacacct
    63241 gttttatttg tttatggtag taaagactca cacataccgg ttgtgcaatc aataaagcga
    63301 ttggaagatt ctatcgaagc cgatcaagtt aattacagaa tcaaaacgta cagaaatgcc
    63361 ggacacctca ttcaatcggt tgaagattcc gtagaatcat tgaaatttag tttgtttaga
    63421 ttcatcaagg gaagacccca acctatccct gaatacttgg aattcgtcag aagatgggta
    63481 ttagcaccat cacaagtgga cgcagataca gttttatgac acccttctaa aaacgtacaa
    63541 cgctacacaa taaattttta agcccacatc tattaggtgt gggcttaaaa aaacctatat
    63601 cgcgaatcaa attacacttc cataatttct ttttctttga ttaccaacat ttcgtctatt
    63661 tttttgatat agttgtcggt cattttttgc actttgtcct gagcgtcttt ggagtcatct
    63721 tctgaaatct tatgatcctt ttcggctttt ttgaagtgct cattggcatc gcgacgtaca
    63781 ttgcgaacgg ccacttttcc ttcttcggca tattttttac cgagcttgac taattcttta
    63841 cgacgttctt cgttgagcgg cggaatgggt atgcgaatca acgcgccatc attttgcgga
    63901 ttgagaccca gattcgcttc acgaatcgct ttttcgatag ccggaaccat acctttttcc
    63961 catggttgta ccgtaagcaa tcgcgcttct ggcgcggaaa cattaccaac ctgattgagt
    64021 ggcgtcggtg tgccgtaata atccactttg acaccatcca gaagtgtcgt cgtcgcacgg
    64081 ccgctgcgaa ttttagcaag ttcattgcgc accgtctcga ggctcttttt cattttttct
    64141 tcggtttcgt gatattgctt attcaggtcg tacataacag cacctcaatt atttaattcg
    64201 gttctttaga tttttattgt tcttgctgaa cgcgtgttcc tacattttcg ccgagtacaa
    64261 gtcggcggag attgccttga gtttttagat tgaaaaccac tataggaagt ttattatcca
    64321 tacacaaggt caccgctgtc atatccatga cgttgagtcc ttttttgacc acatccagat
    64381 aagtgatctt ttcaaaccgg aacgcttctt tgtttttctc cggatcggaa tcgtaaacgc
    64441 cgtccacccg cgtgcctttg agtatgacat cggcctccac ttcgatcgca cgcaatgacg
    64501 ccgctgtatc cgtcgtaaaa tacggattgc cggtgcctgc cgcaaagatt acaatacgac
    64561 ctttttcaag atggcgtatc gcgcgacggc gaataaacgg ctcgcaaacc tgttccattt
    64621 taatcgcact tagtatccgc gtaaagagtc cttgtttttc caacgcatcc tgcaatgcca
    64681 gcgaattcat cactgttgcc agcataccca tgtgatccgc cgatacgcga tccatacctt
    64741 tggcactggc cgctaagccg cgaaaaatat tgccaccgcc gattacaatg gcgatttcga
    64801 cgcccaaggc tttgacatcc ttgatttctt tggctatctg ttcgagcact ttggtatcga
    64861 ttccgtatcc ctgttcaccc ataagcgctt cgccgctgag cttgaggagg attcgtttga
    64921 atatgggcat gggcatggta tgattccttg tatttgcgat acgcctgcag ttcaggactt
    64981 ctattgaaag caggtaaaat tataaaaagg acataaaaaa agccacgaat taaaatccgt
    65041 ggcttttaaa agattattca ccgaggcgaa agcgttcaaa tctccggatc gttaccggtt
    65101 tgcctaattt tttggagaga tcggcaacaa ggtctttgat cattttattg ggatctttta
    65161 cgaatgcctg gtcaacaagg cagacttcct gataaaattt atcaaggcga cctttgatga
    65221 tgttagtcaa aacattgtcc ggtttatttg catttttcgg atcgtttttg agctgttcgc
    65281 ggagaatggc cgattctttt tcaaccacat ccgccggaac ttcttcgcga tggatatacg
    65341 tcgggttcat cgcagccgtc tgcatagcaa tgtcataagc gacggaatac gcatccgcag
    65401 tcatcgttgc ggcaaaacct tcgacattga gctgcagaag cacgcccaac ttattaccgg
    65461 ggtgaatata acccgcaata acgccttgat tgttctcgat cacggcaatg cgtttgacgg
    65521 cgattttttc accgatttta gcagtcaatc cggtcacggc atccgctatg gtctgacccg
    65581 cagcaaaggc cgcaggggca gataaaaatg catccagatt ggccggcttc gaatcacgca
    65641 cttgcgccaa tacgttattg gaaaatttta cgaaatcatc atttttagcg acgaaatccg
    65701 tttcgcagtt aatctctacc atgaccgcaa aattgtgcgc atcgttgacc gaggaaagta
    65761 cggtaccttc gttggcaaca cggctttgtt tttcggctga ttttttgaga ccgcgttcac
    65821 ggagaatttc taccgctttg gcggcatccc cgccggcttc agtgagtgct tttttgcact
    65881 ccatcatacc cgcgccggtt tcttcgcgta atttcattac ggccgaggca ctaatttcta
    65941 cagctgacat atttttataa aactcctgtt ccgaggtttg taaaattccg gtacacggaa
    66001 ttaatcttct tttttcactt cttttttacc aagacgcttt ttgttaaaat tcgtctcttc
    66061 atacttcata cgggtttcat ctttgactct ttcttcatcc acttcggcga tttctttttc
    66121 tttagcggca gctacggcgt gttggttggc ttccgcaacg gcatcggcta tagttttggt
    66181 gatcgcttcg atggatttgg tcgcatcatc gttaccagga attacgtgct gaactgtatc
    66241 cggatcgcaa ttggtatcca cgatagcaaa tacgggaatt ccgagcacat tggcttcttt
    66301 gacggcgatg tgttcttttt taacatccac gacaaatacc gcacccggta attgtttcat
    66361 atcttcgata ccgccgagta cgatcttaag tttggctttt tcgcgctcca acatgaggcg
    66421 ttctttttta gtaatctttt caaacgtacc gtcaatttct tttttctcga tattcttcat
    66481 gtgtttgatg cttttacgaa ttgtcgagaa attggtgagc ataccgccca accagcgttc
    66541 cgtcacaaag aaggaattgg cgcgtttggc ttcattttga actatttctt tagcttgttt
    66601 tttggttccg acaaaaagaa cacgttcacc gttggcaaca atttttgcca gcgctttgca
    66661 agcaccttcc agtgaatgga ccgttttacg caaatccaaa atgtgaatac cgttcttttc
    66721 catgaaaata tacggtttca ttttgggatt ccaacggcgg gtcaagtgac caaagtgtac
    66781 gccggccgct aaaagttctt ccgttttctt tttgggatca aatgagactt ggacttcaga
    66841 catgagaaac tccttacagg gttaaacgac cactttcgtc gcttttgagc gaaactccgc
    66901 cggtagcggg gcaccctcgt tcaaatcaga aagtgtgatt gtttgaaaat acgcactgaa
    66961 tgattcatac agtgctttgt ttaaaaatat aaaagaagtc ggtcacacac atagcgcgta
    67021 tgaccgacac caatttttcg aataacgaat taacgtttgc tgaactggta acgcttacga
    67081 gctttagcca aaccgtattt acgacgttct ttcatacggg gatcgcggct cattgcaccg
    67141 atgggtttca gagctttttt catctcttca tcgattttta cgagggcacg tgcgatacca
    67201 agacgaatcg cgccggcttg accactcggt ccgccgccac gggtcaaagc aatcacatca
    67261 tatttggaac cggcttcgac taccgtcaaa ggttcggtca cgtgaatctg atgcgatccg
    67321 cgtggaaaat aatctttgat gtcgcgatgg ttgatcgtca ccttgccgtt gccgggtacg
    67381 aggcgaactc gtgcggtcgc ggtcttgcgg cggccaacag ctgctaatgg tgcgctcatg
    67441 gtcactcctg tttataaatt aaaattttaa cgtttcagga ttctgagctg aatgcggatg
    67501 tttttcgccc gcataaattt tcagcttctt tatcatttta gcgccgagcg tgtttttggg
    67561 cagcatgcca tgcacggcca aatacacaac gcgttccggt ttggtacgaa tcatatcttc
    67621 aaacttagtg attcgttgtc cgccgggata catcgtatta tgaatgtatt ctttgatttt
    67681 tgtttttttg ccggtcaagc gaactttgtc cgcattcact acgaccacaa aatctccgca
    67741 atccatatgc ggcgcataat acgctttgtt tttgcctttg agaattgacg ctacacggct
    67801 ggcaaagcga ccaagtgttt ggtcagcggc atccacgacg aaccacttct gtttgatttg
    67861 gtcggccttg ggcatcaatg tcttgttcga aactttcacg gtataaactc cttagagcct
    67921 aaatgtataa aaatattata acttacgcat tttaggggac gcaatataat aaacgaccta
    67981 agacaatgca agaagttttt aataaaaaag taattgatta ttattcatgc aataaagtcc
    68041 gtttttgcaa ttcgtatcct ttattctaaa ctccttttaa acaacactct atcagctaat
    68101 tttttaatgc tgtgtttgtt caatcataca tccgaacatt cttttctaaa aaactgcgca
    68161 ttgtttcagc ggaacggtta tcatgcacgg caaactgttc cagctcggct gtatcgtttt
    68221 tcaagtaacc acccggttgt gttttagaac cgacactgat tgaggttacg ccaagcttca
    68281 atgtgcgatt gcgaaaatct ggtgattcgc gcgtcgacat cgctatatcc caatccggat
    68341 tgatccatcg aaataccgca accaaatgat gcaaatcact ctcaaataca tcggagactt
    68401 tattatgcac accgacagca ggtcgaatac gcggaaatga aatagcgatt tgcaacttgg
    68461 gatacgcttc tttgagatac atcgcatgca tggctaaaaa aaacgcatcc acacgccagt
    68521 tctcaagccc gagcaaaaca cctaatccga ttttcgaaat ccccgacatc gcgatgcgat
    68581 ccggtgtttc aagccgatgg cggaaatgcg acttcattcc gcgcggatga tatagacggt
    68641 agttagtttc atgatacgtt tcttgatata tataaacccc ttcgcacccg gcttcgcgga
    68701 gcaaagcata gtcatccgta ttcaacggtt gtacctcaat ccgtacatga tcaaacaatg
    68761 ttttggctat acgcacagcc tccgcaatat aagttacgcc tgcaaaacgg cgctcttcgc
    68821 ctgtaaccaa taacacacga cgaacacccg tcgccgctat cgcttgtaat tctttttcga
    68881 tctgtgtttg atcgagcaca acgcgttgaa ttttattttc tacgcgaaat ccacaatacg
    68941 tacaactgtt gtgacaataa tttgacagat aaagaggggt gtacaaatga atcgggcgtt
    69001 ggatttgctg cgcggcttgt tgtgctatta catgacgata tgtgtcagaa gccggagaaa
    69061 gcagagtaat aacgtcttcg ggaaatttaa aatcagacgc atcggcaagg atattttcta
    69121 ccgatttttt tgttcgtgtg cctaaaagat tctctactac aggccagcga taagattgcc
    69181 aacgtgtata aaaggacata ttgagattaa tgtttggcac aacaggattc accgctgccg
    69241 gcagccaccg cgtgcatatc cggactgata tacgggatgc caagagacag cccccggaga
    69301 atcaataaaa cagccacgcc acctactacg acaggaatca gtttagtgat tttctggcgc
    69361 gttgtggtgg taatgaattg accaaaaatt gcaacagcgg tcattgccgg caacgtgccc
    69421 aatccaaaca atgccatata caacgcacct tcccacgcat gacctgtggc catagcaccg
    69481 ccgagcgcta tgtagactaa tccacacggt aaaaacccgt tgagtaaacc cagtgtgaag
    69541 agtgatgtgg gattgggatc acgcagaagc tgaccaaacc atctccgcat cgctgcccaa
    69601 ccgccctgga gcgatgagtc cggccatatt ttttgtttga tcgaattcgg caaaaggagc
    69661 gctaataaaa tggccacacc aagcccgatc gataatcctt gttgaagccc aacggtctgc
    69721 agcgtcgcac caattgcccc tacaaccaag cccatcatgg cataggtaaa agctcggccg
    69781 ccattgtaca gtaatgctga tcttaccagc ataaaccacc ggcgatcatg ctgcggtatc
    69841 gctaatgcca atggaccaca catgccgata cagtggaaac ttccaagtac acccaccaat
    69901 aatgctgagt atatttcaaa cataataagt gactcgccga tttattcgat gtaaattaca
    69961 tcttcaagat aatagtcatt attttccgat ttccattcta actttgcacg ccacatcccg
    70021 ggtattgctt tttgtaaatc tatcgcttgt gatcgttggc tgtcaagctt cagaggtacg
    70081 acgatatccc gatcagcatc cgatgcacga tacaaggtca gtgtacctcc tgtgatttgt
    70141 gagatttgct caggaaaaat catcgttaac acacgcccag aaatattcca tgccggtttt
    70201 tgttccaaca tcttcgtacg tttgatcgct tgaattcgct tttcataagc aatctcattg
    70261 tcgtaatagt tggtacttac caaatcctca cgactgatgg aggaaacgaa taccattcca
    70321 agaatgagaa gcgcaaaact gacatagagc acagtgattc ggaatcccca gtttctgaat
    70381 aacgacatgc ctcttccttt cactgcatgg gcgacacaaa cgaagtttct tcggttgaaa
    70441 ctttcattcc gtctttatac acacctattt taatttttgt attcattgta tgaatagaat
    70501 tacgcggtat atctactaaa aacgtggact ctaccaattc gtccggatgc aaccgcaatt
    70561 catgggtacc gatcagcgca atattccctt ccacaccttc gagcttaaac tcaatctgca
    70621 ttgcatcgaa cgtcttattg agtactttgg ctgtatacac attccgtata tcaccattct
    70681 ccataagttg gaagataggc cctttcgcac gtaaaatcgt agtttgaatg tctttacgtt
    70741 tgaaaaggaa aatggacgtc actgtaatca atacagtcag aacagccgaa tataagacaa
    70801 tacgcggtgt aattttgaaa tgcaattttt gcgaaataag attatacgaa ccgtagcgga
    70861 tgaggttttt aggacgcttg attttttcca tgacagagtc gcaagcatcc atacatgccg
    70921 tacagttaac gcattccatc tgcgtaccgt tacgaatatc tattcctgtt ggacatacct
    70981 gtacacataa tttacaatct acacaatcgc ccaaactttc gccgccggta ttcccttttt
    71041 ttaattttcc tcgaggttcc ccgcgctgaa aatcatacat gacaacgatg gagtttttat
    71101 ctagcaatac cgactgtaaa cgaccataag gacatacata gatgcacgcc tgctcgcgaa
    71161 aacgggaaaa gataccatag aatatcaaac tgaaaatcat catcgccgta aaaccgttga
    71221 catgcatacc gatcggatcc gtgatgattt tgaataattc atctacaccg ataatatacg
    71281 cgagcaaggt gttaccgata atccatgaga tcgcgaaaaa gataacatgc ttaaatgttt
    71341 ttttgaggat tttatctgca ttccatggtg cttgatccaa tgcgtattgt tttggtccgt
    71401 caccttcaat ccaaaactcg atttttcgaa aaaccatttc cataaaaatc gtttgaggac
    71461 acgcccagcc gcaccagata cgcccaaaca cggaggtgaa gagcactata aatatgacaa
    71521 tagaaagaaa cgtaagcgca aaaaggtaaa aatcctgagg ccaaaaggcc aaaccaaaga
    71581 gaataaattt tcgctcaagt atattgaaca tgataaaagg gtgctcatca actttgataa
    71641 acggcacacc aaaaagcacg aacaatagaa tgatcgaaac aatcactcgc cagtagtgaa
    71701 atactccggc aacaagtttt gggtaaatcc aaatacgtgc gcctttttta tcaataatcc
    71761 cgatacggtc acgatacgtt tcaccgtccg cgttcttgat ctgcgctaat tcctctttta
    71821 cctcagccat actgccgcgt aattaacttt ctttttattg ttttaccaga tcgccttgag
    71881 gcgctttggc attcggtgga ttggtaccac gtagtgtaag aatataactg gccaccaata
    71941 acacattttt ttcgccaagt tgtttccaag agatcatacc tttttcagga acacctttcc
    72001 atgcggtacg cacgacatta ttgatacccg caccatgaat ccaataatcg tctgtcagat
    72061 tgggtccgat accgccttca cccagtttac catgacaggg cacacagttt acggtaaaaa
    72121 tctctttgcc tttaccgagg cttactgcat ccgtcagtgc ggcaaattct ttggtgagtt
    72181 cttcttcttt tggtttttca gcggccggca taaaggctac aacaccttta ttggtcggat
    72241 cgcttggctt atagtaatac gggcctttat aaccggtacc aagcatatca actttttgat
    72301 aatttggatc catttctttg gtccatgtat tgtcaaattc ttttctgtat tcatccaccg
    72361 agcggtcgcc gatttccaga acatgataaa atatcaagta aatggtcgcc caaacgatcg
    72421 tgatgtaaaa catattgacc caccagggcg ggagtggatt atcatattcg cgaatgccgt
    72481 cataatcatg atcgagcaac tcatctttga cttcagccat attattttcc tccgtgtttg
    72541 tatccgttgt ttgatacagc gatttcttcg ccatcgagcg gcatcgtttc catatgatgc
    72601 agatgccgct tatctatgcg cacaagccat gcgatcagtc ccacaaaaaa aacaaagaag
    72661 atcacgagcg ctatggtcgg ccatatttcc agaccacctg cattttctaa aattcgacgc
    72721 aacatttatt gtttcacctt gatgtcggtt cccagtcgtt gcaaataggc aatcaatgca
    72781 acgatttctt tatccggtgt cgtaggaaat ccgttttgtg tcagatcgcc tgcgacggct
    72841 gttgcttgtg ctttcaaatc attatttgct actttttcat atcctttttc atacggcaca
    72901 ccaacgatct gcatagctga aattttcttt tccgtcgttg atatgtcgag gctgttttca
    72961 aaaagccacg gataggccgg catgatcgaa cccggtgaaa tggctctggg atccatcatg
    73021 tgactataat gccacgaatt cgtgtattta ccaccgacgc gatgcaaatc cgggcctgta
    73081 cgtttagaac cccattggaa cgggtgatca taaacatatt ctccagcttt ggagtattcg
    73141 ccatagcgtt ccgtttcaga acggaacgga cgcacaagct gcgagtggca agtataacat
    73201 ccttcgcgga tataaatgtc acgtccctgc aattctaggg gtgaataagg cttgacgctg
    73261 gcaatggtag ggatattaga ctctactgca aacgtaggta cgatttctaa aactccaccg
    73321 atagcaacgg cgatcagtgt caaaatgata aacggtaaag gtttgcgttc gatcaaacgg
    73381 tggtaatacg attgaccgtg tgacacatat tcttttgtta atgccggcgc ttctgctttt
    73441 tcattggcaa aagaatatgt gtcacac
//
LOCUS       DAOQGF010000004        64110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_142_length_64110_cov_6.400781, whole genome shotgun sequence.
ACCESSION   DAOQGF010000004 DAOQGF010000000
VERSION     DAOQGF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 64110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_64110_cov_6.400781"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK937_01555"
     CDS             complement(<1..1087)
                     /locus_tag="PK937_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH31292.1"
                     /translation="MMKSTIYIGLLWFAIAEYYSQTGDSVKIKQYDLQGTVITSSRLI
                     NSRNASSALVNVITEENIQRRGATNLSDALSGASGIQIKDFGGTSGLKLPSFRGSTSE
                     QVIIMIDGIPLNNPATGLIDLGVVPLTMIDHIETYKGGDNNAVGGTINLITKKQPRDE
                     PMSIITKAHYSSFKRRWLSAGLTGFIKQTSYSVAYEFEYQPDSSYFVTVPSSGQRVPR
                     VNSESKRHALFLNVNNRLSLKTDFSLMSQWTFRDVEIPGTISNNTSVTSRGNQEDRVF
                     LIAPQIQHRFNEQWLTAIGSSWYKYQFDYTNSFPTVIHSRNISNSYHTRWTNLITLLK
                     QHTFNLGVTHTFVQGEGDNTDHVSNQSV"
     regulatory      complement(1170..1353)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1410..3779)
                     /locus_tag="PK937_01560"
     CDS             complement(1410..3779)
                     /locus_tag="PK937_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH31293.1"
                     /translation="MRLFLWFLFVMSQINAQDHILLDSVDALLRESKYADAIHLFEVN
                     SISTIHKPATHAALSKAYFYLGQNKESQSLYSSLLLRKDVATMDRFRWTQDYILVAAE
                     TGNFKLCDSLLTIIQSILPTVKDSTALARYWNTVGIIYIQSSQPFLANEALNKAEHYM
                     HRDLDLKLQSWIVSNRGIVYKMMGDFASAIEKYRMALYQDSLRNDKADMAADYQNLSL
                     IFSEMQLYADSRYCFEKALEYIRMTGDVGTEGIILNNMAHLFNNHGQIKSALQCLRSS
                     LVITEKAGDKIATADAYYELAGAFRALSQYDSAIYFWNKAYDIYCEIGSIDQTFFTLL
                     EKFRAMVLLKQFKQGDQLLEQAFNLLSQLPSKRYSPSYYDARALYFESINQTDSADAY
                     YVLALQSIDSIYQEQNSAHVYDASIPNDRTRFYKNYIRFLFDQKKYHEALSYQLLLNS
                     RSTHNTKPHPILFFESGVSFFQADTVMHVLLNTERELQYRRLCITPLIKDHLLQIKTL
                     LRQPSANQHSLSQHLAVLSDWINPAIIDYVQSHKELVIVPDGLLCNFPFEMLSLERRY
                     LTETHTIQYTAALNSDEKFSIYSERTIPSVTILARSEYSSWTSLPDLPDVDNEIDRIK
                     SIIPHSQIYYQVTEKSYYDIIGTDSSQVLHLIGHGMNATDDARKSGIILGRDQIHHDG
                     YLTCNEIEKQQLRGQSVILSACETSVNTKISQKNIPAIPEAFLNSGASLVLSTLWKIN
                     DRDAVNFVDQLYQHWVIEKSIPAALQKTKRDFIVQNTLPFYWASWVIWK"
     gene            complement(3785..5557)
                     /locus_tag="PK937_01565"
     CDS             complement(3785..5557)
                     /locus_tag="PK937_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNH31294.1"
                     /translation="MYFSKIQMYSWVLLITVMTIACKNDEKKTDLNDLRTVLAVVGED
                     TVFLGDYKNRVSELKQDKPDLESEIIRRQILDEMVNYYLVVMEGRSQKLQDHPYVRFQ
                     VRTKEDEFYFAHAIRNAIVEPSITEDQIKARYEQLKEQLDVKHIFIGYDKTFDPSLEM
                     APNQKKIIRFKLAAKSIADSLLGVLKKEPDKFENFIEQFSDDAATKFFDGTIKGLRRG
                     ELPNEIGDALFKLQPDELSQPIDDGNGYHIYKIIQKSNDANVLPYEKAKIAIKEQIAK
                     QILLKPDIQIEKNKKEFIENRLRDGSYSFNQSNAAAWYSKFKKYDSKKPLVDQLTDDE
                     LNTILATSLTDTIKIVDIAFALTGNRFNSRITIEQMESGIRNAIAVRLVSRWAKEQKI
                     ELNDREKRFIKRIESGLISTKVQPPSVDIQLIEDTSNVRHYYEANIKNYKMPDSLDLG
                     MIVMNEEAQIRPIADKIKSTRNFEISFEELRKKNPASAVRSGLRPLKEFGNLSARIQE
                     LKEGDISDVFIMNDGHYGIVKIYKRTQPSWYSLDKVFVQVKSDYSVSLIKQTRDKWLL
                     SLKEKYTPTVNYHSLKDAFEIKLK"
     gene            complement(5577..8978)
                     /gene="mfd"
                     /locus_tag="PK937_01570"
     CDS             complement(5577..8978)
                     /gene="mfd"
                     /locus_tag="PK937_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNH31295.1"
                     /translation="MLDIVLQRITSSESAQNLLRHIAEKKTIMVDGYLGSMDAVLGIL
                     AAQSAYVNDRLSVIVVPDSLTYGRVLDDLTTLQDYTTHKIPILQFPEDELLPYDDREA
                     NPAFVAARQESIEQLLNNEKAILVTTVRALSKRIPGPRSVASYKIELQSGATFQFQYL
                     VELLHEIGFEKQEIVDQPGTFAIRGGIVDVFPFAQQNPIRIEFFGDTIDSLRFFDVVN
                     QRSLQKTDYLALYPKSESQTAQHDADLTDYWPQDTLVMLLEPEILQGALDKYWDEIND
                     SYQRKSEAHSELLAPESRFLDTASVMKKLNAFACLYFARYSRIMDFDKINFTAHALES
                     FNSHINLLKERLIAIRGQYKVFIACNNTGQAERLSELLDSDELASGVDFVIGVGELHE
                     GFYTEDAQCVVFTDHQIFGRIKRTRSHRKFKTAQALRHIASLKPGDYVVHVDYGIARY
                     AGLEKVTSGEHTEECLKLLYKNGDKLFVPLEHFSRVQKFSAEDGVTPTLHKLGSGDWD
                     KVKARTKKTLKDIAQDLIKLYAERKSRKGFAFTPDTHMQYALEASFEFEDTPDQTKVT
                     LDVKKDMESDNPMDRLVCGDVGYGKTEIAIRAAFKAVQDSKQAAILVPTTILAEQHFE
                     TFSSRMREFPVRIDVISRFRTTKEQKIILEKVQRGEIDILIGTHRLLSKDVQFKDLGL
                     LIIDEEQRFGVAHKEKLRQLKATVDTITLTATPIPRTLHFSLMGGRDLSVINTPPQDR
                     LPVKTEITQFDEDLIHDAVMKELDRGGQVYYVHNRIQSIDRITGMLNTVVPKARLAVI
                     HGQMAPQQVEDIVHAFMKREYDILVATTIIENGIDIPNVNTIFIDQAHQFGLSQLYQL
                     RGRVGRSNHQAYCYLLTMPMSVMPSDALRRLQAIEEFSDLGSGFLIAMRDLEIRGAGN
                     MLGAEQSGFVNAVGFELYCQILDEAVQETRQEMNVVESGKKEEITQQAEYIPALQVHV
                     FCDTYIPDTYVNVPSERVRIYKSLSDIISIDALNAIEQELVDRFGKLPVEAKNLLQMI
                     EIKLMTLPLCAERLDIDENKLVLRFDVKRMSQPNVADRMKLKMNSIVKKFENLRFFQN
                     KDFLDITFGFEWYDATKSRIISTESKEDSLVLISAKKIISQIIEL"
     gene            complement(8994..10451)
                     /gene="miaB"
                     /locus_tag="PK937_01575"
     CDS             complement(8994..10451)
                     /gene="miaB"
                     /locus_tag="PK937_01575"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNH31296.1"
                     /translation="MSDTAFEEIHTPESIADVRSRLQGNRKRLYLETYGCQMNVYDSE
                     LVASIMRDMDYDLVDDYETADAIFMNTCAIRENAEQRVWGQLTRFKKLKEEKPHLIIG
                     VLGCMAKHLEEEIHAKRPYVNVVLGPDSYRKIPELIEKKQASQNLVQIRLPSQHGEDF
                     SLSEYRDFQLKPLQMDTRLSRTEVYDQVEPLRLGKITAWIAIMRGCDNFCTFCVVPYT
                     RGRERSRSVESIVEEVHKAVAQGYTEIGLLGQNVNSYRDPQQKDFAELMNRVSEVPGV
                     RRIRFTSPHPKDFPEHLLHIIAQRDNICKQLHIPVQSGSNRILELMNRTYTREEYLKL
                     IDTVRRIIPDVSISTDIITGFPSETERDHLDTLELFRTVQFDSAFMFKYSARIGTKAY
                     TMPDDVTEAEKSRRLQQIIEVQKENGLKSLHREIGKEREVLIEEVSRKSDNYYCARTD
                     KGQIIIIPKSDKTIGEYARIRIARADGHTLFGEYI"
     gene            complement(10456..11010)
                     /locus_tag="PK937_01580"
     CDS             complement(10456..11010)
                     /locus_tag="PK937_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31297.1"
                     /translation="MKKKKKTQPAVSKRVKSVIKKTDKTRSVALKTKKTKSEDEHKFN
                     LSPVDVERIAKSLGVEKQDFGDHYRMYLENQTEGRKLTLEIYPEIPIGRRKGTLITVY
                     TPNAHLQLHFCSGYVVSELLNEVTFVGVQNGKLSGLTIEKGAACSLYANVDTEMLSGD
                     LTTLGPEVMLSGIALSLTDSLINE"
     gene            complement(11007..12443)
                     /locus_tag="PK937_01585"
     CDS             complement(11007..12443)
                     /locus_tag="PK937_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31298.1"
                     /translation="MLQTDIDKAIKLLESKVSVFKGDFTLADASAVSGLSVDEAKSAL
                     DAMMVKYDCRLRLTENGDLIYGFGVSLRRRGAKTWKDYVAAIGQIFWKIFTWFYKAWI
                     AITLVVYFGIFLVLLIAMVVAAVAASKDSKRSPVNLGKIFDVFFSIFRWRTITGDIGY
                     KVDNRGYRYKNYTPRPSPLNENKKSFMASVYDFVFGPERVQIDPLANAREVAAYLRRE
                     KGIITPAELIALAGWTREKAESFLSDCIVRFQGEILFTDNGVIYGRFDQITRKKESAQ
                     DGAIEYYWDEYEPDYELTGNTTGRNALISFMNIFNLAFSVLILKTYYRPLEDMPWMNE
                     SPWIGVFLGWVPFVFSLIFFIIPLFRSFAIIKQKRNRRAMNVRKRIMRYLFSMPSASA
                     TVDEVVTHINGKSKGEEVLKKHDVQEALDSMLRDFNGETTLNNNGDVRYTFPQIEQEL
                     KESELLRKNRSVDNDLGAVIVDTQNTQS"
     gene            12554..13234
                     /locus_tag="PK937_01590"
     CDS             12554..13234
                     /locus_tag="PK937_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH31299.1"
                     /translation="MQNTILRDTLKVNEIFYSIQGESTHAGKPCIFVRLTYCNLRCTY
                     CDTEYAFYSGRDMTVEDVIRTVQSYTCKTVEVTGGEPLLQERVYDLMIRLCDMGFEVM
                     IETGGSLDVEKIDPRVLKIVDMKCPSSGMQDKNYYPNLNKLLPTDELKFVIGDRNDYE
                     WAKALIAQYPHTKQINSILFSPVFDTIEPVSMVNWMLEDKLQTIVPNIRFQIQLHKVI
                     WSPETRGV"
     gene            13234..13668
                     /locus_tag="PK937_01595"
     CDS             13234..13668
                     /locus_tag="PK937_01595"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNH31300.1"
                     /translation="MTKTVKIAKEFHWEMAHRLPYHTGGCQNIHGHSYILWVEIEGQP
                     QENGMLLDYAEIKRIVKPMIDMLDHGFICSRDDKAVLAFLNNSKFKMTIVDFYTTAEN
                     LCYYFADAIEKEIIKYPHVHSMKIRICETRSSYAEITRSLKR"
     gene            13665..14369
                     /gene="queC"
                     /locus_tag="PK937_01600"
     CDS             13665..14369
                     /gene="queC"
                     /locus_tag="PK937_01600"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNH31301.1"
                     /translation="MTTDTHSTLAVVLASGGLDSCVTTAIAAQTHALAMLHVNYGQRT
                     EKRELRSFNDICNYYSVLPSRRLIVNIEHLKQIGGSSLTDNSIPVSSADLHNKSIPSS
                     YVPFRNANILAMAVSWAETIKAEKIYIGAVQEDSSGYPDCRSDFFEAYNQMIRLGTKP
                     GSGIEIITPIIHLQKSEIVREGLHLNAPLSLTWSCYQSEDEACGVCDSCALRLRGFQS
                     AGIEDPILYRVRPKYD"
     gene            14435..16411
                     /locus_tag="PK937_01605"
     CDS             14435..16411
                     /locus_tag="PK937_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HNH31302.1"
                     /translation="MGLGKRVLYALAVFIFQAYITGCAAGFQDIRQTQKDYGIEKLPS
                     AEEYPQHEAVIVLESHKIDVSIDNNFDVFTIEDVHVVKRIFRNVENHAEVEITLGYGE
                     KLKSIQARAITSTGQILELEKKEFLHSKGSSDGSVFYSDDRTTRFTFRGVDKGSIVEY
                     KYRIQKDYPFQMDVWYVQGSLPILRNEYKLFVPTILLDNNWNWFFKSYNYDIGLPKIE
                     KAGISANKTLDSKNTYTWLVQNVPAFEREPRMNSYYHHAGHVRFAPSYWNSWNAIADW
                     YYKKLFLPQLIVTPQISEKAQSLIANVADEKSKVQRLSDYVKKIRYTAIELGIGGIRP
                     NTPQTVYDRQYGDCKDKATLLIAMLKSVGITAHPVLVRTANSGFVDAQFPSWNFNHMI
                     VKIALSGGKTIWTDPTSETSQFGFLPWSDEDIDVFVLYPDGTGMIEHTPTSSHTNNLT
                     DVHIQGEAIGDMGMKFEIKIDFFGNDYSYYLYRLRDMSQNELENFCKNLIVDEYLNSK
                     ISNVRFSENDTAGKPLSLVFETTVEKVIQAQGDLYFANIDPFKLFESWSWLASEQRRF
                     PIRTIYPYLIKKRIEIKFPANLTIRNLPKPHNESIDYFSYNRSYQQAGNDALIFNETY
                     AVTERSVPAIEYNTVRKFFDKIKSGMAEKVILTK"
     gene            complement(16481..16572)
                     /locus_tag="PK937_01610"
     tRNA            complement(16481..16572)
                     /locus_tag="PK937_01610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16536..16538),aa:Ser,seq:gga)
     gene            complement(16666..18120)
                     /gene="gatB"
                     /locus_tag="PK937_01615"
     CDS             complement(16666..18120)
                     /gene="gatB"
                     /locus_tag="PK937_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNH31303.1"
                     /translation="MQYEPVMGLEVHAQLLTQSKAFCTCSTKYGAMPNTQTCPVCLAL
                     PGALPVLNKRAVDFAIRMGLATHCVIAEESIFARKNYFYPDLTKGYQISQFDKPLCEN
                     GWVDIEVGEAVKRIRIKRIHMEEDAGKSIHDDALTGGEKTFVDLNRCGTPLIEIVTEA
                     DFRTPAEVVAYLTKIRQTVQYLEICDGNMEEGSLRCDVNISMRPVGQEKFGTKTEIKN
                     MNSFKAVERALNYEMARQTDILQNGGSVSQQTLLWDDNQGVSRALRSKEEAHDYRYFP
                     EPDLVPLRVTKAWVDEIAGSLSELPGARRDRFVQQYGIPKYDADVLTLSKSLANYFEE
                     TLKHVSDGKLASNFIMGEVMALLKERKQDADAFIISPANLGKLLRMVSDNTISGKIAK
                     IVFEEMAKDGADAEAIVKTKGLLQITDRGEIEKIIDRMFELNQKQLQEYLGGKEAVFG
                     HFVGQVMKLSQGRTNPAMTNEILKQKLAEKKQNQ"
     gene            complement(18140..19414)
                     /locus_tag="PK937_01620"
     CDS             complement(18140..19414)
                     /locus_tag="PK937_01620"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNH31304.1"
                     /translation="MSVRIVIGAQWGDEGKGKIVDLLASQAHVVARYQGGANAGHTIV
                     HGNKKYVLHLIPSGILHEGVMCILGNGVVIDPIALFDEIKMLSEMGIAMHDRLMISPH
                     AHIILPYHKLIDQAKEKYLGENQVGTTKRGIGPAYVDKIDRSGIRAIDLIHPDTLERK
                     LSKAITEKNKIIKAFGSEELPIEPTIKQFKEIGQQLKPLIGDSVKYLFESNQTGKEIL
                     LEGAQGTLLDIDHGTYPFVTSSNASAGGACTGLGIGPSYINHVMGVVKAYNTRVGNGP
                     FPTEFDEVFGQKVRELGGEFGATTGRPRRCGWLDLVQLKYAMMVNGIDELAITKLDVL
                     DTLEELKICVGYKVDGKRIDHYVTDAGELERVEPVYETVSGWKSDTSGVKNYVELPQK
                     AQAYLKFIEDYLGHARIKIVSIGQEREKTFMM"
     gene            complement(19494..19832)
                     /locus_tag="PK937_01625"
     CDS             complement(19494..19832)
                     /locus_tag="PK937_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH31305.1"
                     /translation="MDYNIAENNGVVVLELKGNIMGGPDATQLNDEIHTRIKQGKKNF
                     VVNMQSVDLINSSGLGILISNLTTVKNNGGDLRLTNLSSKIKQIFQITKLATVFKQYD
                     SVDDGVKSFV"
     gene            complement(19899..20789)
                     /gene="secF"
                     /locus_tag="PK937_01630"
     CDS             complement(19899..20789)
                     /gene="secF"
                     /locus_tag="PK937_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNH31306.1"
                     /translation="MELFVNKNYPLVDTRRNAYIFSGLLILISLLLFFFRGLNYGVDF
                     VGGTSIEVKFSSKVELEKVRSIVSSKYSSDRIQDFGNGNEVLIHVLEQNNDVAPTVLK
                     LMNDNFPGNSVELRSVSQVGPKIGDELKVSAIWASIWGLLFIVVYLAIRFHWKWGMAA
                     VAALFHDVIITIGLFCALQLEFSLGAVAALLTIIGYSVNDTIVVFDRIRENTRLIKRG
                     TTFGEVINKSINETLSRTVMTSALTLLSVIVLLMIGGEVIRTFAWALLTGIVVGTYSS
                     IYVASPILIEWNAGEDIKVK"
     gene            complement(20819..22753)
                     /gene="secD"
                     /locus_tag="PK937_01635"
     CDS             complement(20819..22753)
                     /gene="secD"
                     /locus_tag="PK937_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNH31307.1"
                     /translation="MIKSKNVRAVIIVICAALSLFALWPSFKFYNLSDAQKKEMLKTP
                     EGTKELNELQSKSINLGLDLKGGIHLVMETDVVTLYENIAKTKDAEFTRVFEKAAEEY
                     RKNPDAVFSNVLRTHFEDAKLSMPAYWGERFKDNADVVKFMDEQSKDAVDRAQQIIRN
                     RVDQFGVSEPTIQKQGDHRIIVELPGVEDPAQAKNLIQQTALLEFKLVKDPQDLGTAL
                     ERLDVYFAKVDTVSGKISAGAVSADTSKKSSDGGTGLSAELQKSSSDSSQKDSSALAN
                     LDKPLTKLLVSSQYDVLVPENNFEYVKSMLYYQVGERVKMKPGVQQVIGESNDIIFSS
                     KPIFEGHHALYLVKKQPELSGAVITEAKQDIYQGMDPGLAGRPIVTLKMTPEGSKRWA
                     IVTGANVGKRIAVVLDNKVQSAPNIQEKISGGNTQITGMENLIEAKSLAIALRAGSLP
                     APVHVMEERTIGPSLGADSIDSGKMAFWIGFVVIALAMVAYYRMSGAVADMALVINVL
                     LLFGVLTGFGFTLTLPGIAGIVLTMGMAVDANVLIYERIREEIRLGKTVRAAIETGFA
                     KAFVTIVDANLTTFGTGIVLFQFGTGPIKGFALTLMIGIVTTIFTGVFVSRFIMDLIY
                     DRSTIERQMSIGISYTKTSA"
     gene            complement(22879..23256)
                     /locus_tag="PK937_01640"
     CDS             complement(22879..23256)
                     /locus_tag="PK937_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31308.1"
                     /translation="MKINRFLSFLVVSTCTILTFSGCDDKPNFPLNGTEWYGEFDGYV
                     NYLDFDKVVYVDAVKADGDTCFDIQFYQYTVDGDDFIIDGYITINWEVVKDTLTLTDD
                     VTSYKFIRTAIDDSSLAAQGCTD"
     gene            complement(23284..24327)
                     /locus_tag="PK937_01645"
     CDS             complement(23284..24327)
                     /locus_tag="PK937_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31309.1"
                     /translation="MRSQFIPILFSVLIACRGAGNNPQPTEQPKTFSIGDGKIVNSVQ
                     FSPDGKFLICGGENKHTKIIEFATGNVIWVSDIMPDAVLVNGFSPDGNRFFITTSDNT
                     QSTGSIGMYSFADRHRQWFIDKQTNDIQLARYSPDGRELIVGNFFNILFLDVETGKQT
                     KFFSGHPIEVPSPYGHYDAVTDIIFTSDPNVFVSVSWDKKILIWDMKAGHDVRTYAEG
                     DPINAMLLFPEGNRILTGSVDAITVWNRTNDAHEQTVVYDGEIKKMCWLEGGNYFVTG
                     DGDGDLTLWKTNGLERLKDISKAQERGIWSMSASPDGKAVVTGGGTGIVTWWPLEYLI
                     QYNAGNADSIKIK"
     gene            24480..25424
                     /locus_tag="PK937_01650"
     CDS             24480..25424
                     /locus_tag="PK937_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH31310.1"
                     /translation="MAVKRPTKAKKTKHVSPKNNSRSKKQPLIKTIANRKVKKTFPVS
                     INVPQIENLLSELEYGEELAEYYDLIYSEKVIHQIQSPFFQMLIKKYGIRSACDLSCR
                     SGQTLAALSKLGIKNLTGVDVSSKMLDLARKRCPSKTQFYQSELFLAPHTIGDETKFD
                     LVLCSRDALPLVLDDEGLLGFMAQARNLLSPNGILVIETWNYDKIWRRKERFMPVMDR
                     CSNKQSLLFFIENDFHGELLVHNVVKLEKNKFEWFLRTISTPIRPLTRNEMEFFVKEA
                     GFEKYGFLGNYAGSPFNTTESAYCIMLAMNNTNTITVS"
     gene            25721..27043
                     /locus_tag="PK937_01655"
     CDS             25721..27043
                     /locus_tag="PK937_01655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH31311.1"
                     /translation="MAFKQLHVIEPILQALKSEGYTKPTPIQEQAIPLILNHKDLLGC
                     AQTGTGKTAAFAIPILQLLFQSSSVHIPRKTHALILTPTRELAIQIGESFTAYGRHTG
                     LRHAVIFGGVSQHAQTQALKVGVDILIATPGRLLDLMQQGFVHLQQLKIFVLDEADRM
                     LDMGFIHDVKKIVGKLPSKRQTLLFSATMPAEIQKLAHSILSHPEQVSVTPVSSTAEK
                     IEQAVYFVNKPDKKNLLIHVLRNPEITSALIFTRTKHGADKVTRDLLKAGVTAEAIHG
                     NKSQNARQRALGNFKNAKTRVLVATDIAARGIDVDNLSHVINYEIPNIPETYVHRIGR
                     TGRAGMSGTAISFCDPEERAYLKDIQKLIGKTIPVMDMPIAVTPAKAAANTLHDHGIK
                     PQQTKQHGRPQHKTGSSDRRTKQKTQHQTGKPKDHRSWFKNTFKKRED"
     gene            complement(27085..27156)
                     /locus_tag="PK937_01660"
     tRNA            complement(27085..27156)
                     /locus_tag="PK937_01660"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27121..27123),aa:Glu,seq:ctc)
     gene            27250..27591
                     /gene="ytxJ"
                     /locus_tag="PK937_01665"
     CDS             27250..27591
                     /gene="ytxJ"
                     /locus_tag="PK937_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNH31312.1"
                     /translation="MTEIQSPEMLDALLEKSLNTTVLLFKHSNSCGTSAYALEQVETF
                     LSEYPEWNSRSGMIVVQTHRPISNRVADLFAITHQSPQIFVIQNKEVLWHKSHHSITS
                     DAIKKIVLSIV"
     gene            complement(27623..28648)
                     /locus_tag="PK937_01670"
     CDS             complement(27623..28648)
                     /locus_tag="PK937_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31313.1"
                     /translation="MKAKTLLLARINFIGIYFACFQTTLYAGTFDWRIGGETGPVISN
                     RTATSRQFLMAAAGSGRYQFVFGEHQLTFQGRGRYEDYNTSFERHAWTSHASTEYVYA
                     SGAWVWSLNTNWRTQQYGLSGPDVRATLYNLQSRGEYYFGQRMGAFAELRFERNISKV
                     DHENRFASRAGVIGLVWSSAEYLKVFTGFLTERFFASTTDPLTYPSKNNGWRNGMETG
                     LDYSGTWIINGRYIASWRVSKYTLNYSLEQEAHIVIGKHINERWGLYFLADYVRRDIP
                     RENKITRNLLYTNSVQDNRAHIKLSYETAANQRLFIRAAYSQTEFTQKVEPFRYKTIV
                     LGYEVNL"
     gene            complement(28645..29577)
                     /locus_tag="PK937_01675"
     CDS             complement(28645..29577)
                     /locus_tag="PK937_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31314.1"
                     /translation="MNRFIFITIGVIGIGRLHAQNMEEKELFYGQTIFAWRASAHAAD
                     SLNQRMKENADEIVQQKKSIQPDKERIARSLAKGLTLSEQIKANQRRMEDYHKRIETL
                     RIQLNSEYDYIIDSLRIQQKRQNTSSIEKQLRYWTEKKLLITPPMNNLSYDPVTLLKI
                     DLYKITDPFEKEVRKEYLQAAIIEVDKYVDQIRTQRKVYESLVSLRKRTTDFIQDAYD
                     AGTWAGFSRNGTSVIKNDGVNNGIDINPQNVQAKTVISVMRQIGNPSNIVRNYEKVLS
                     MSEFMSESDYIQLLKEADKQLVAYRDLLTDKLNP"
     gene            complement(29574..30938)
                     /locus_tag="PK937_01680"
     CDS             complement(29574..30938)
                     /locus_tag="PK937_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH31315.1"
                     /translation="MKIMIVEDNASMRMGLVESLRREGFEVHAFSDGESALKFFKQKP
                     LSLVITDLKMDGMSGMDVLEQVKTIQSQTAVMVVSAFGTVETAVHAMRKGAADFLTKP
                     FSPEELRFRVGRLVEKIRQEIILTRLKEENELLQGELLRSYGAMIGTSQGIKKIFNLI
                     DRVASEDSTILIEGESGTGKELVARAIHQKSNRHDKPFVKVNCGALSENLLESELFGH
                     EKGAFTGAIKRKKGRFELADGGTLFLDEIGDISQAMQVRLLRVMQEREFERVGGEDTI
                     AVNVRIIAATHRSLSKMVQEEKFREDLFYRLRVIPISLPPLRERQEDIPLLVHHFLNQ
                     YAGTRGQSSKQISDEAMETLKKHAWPGNIRELQNLIERLCVISENEIIDDAMITQHIT
                     GPSKSLMSGFENLPLEQALEAFEKGIIMAALKKSNNIKNQAAKLLGIPTSSLYYKMEK
                     FQLL"
     gene            complement(30954..32336)
                     /locus_tag="PK937_01685"
     CDS             complement(30954..32336)
                     /locus_tag="PK937_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH31316.1"
                     /translation="MRTRIGVLFSVITMILIIVLSRFSYVAVREVYLDQLSRQVRMSA
                     KAIATDMDMGNIKFVQPYSENSVATQFYKRYLKKKKVGHELASAFIFDAALRMVMTSD
                     DESDYIHPDPTLLLSQSEIERLAPGETSTTLPFLGNDNEWYMWGFCRIDDEYWAGVRA
                     KADQLMYIENLSFVFWFISGTALVFTLFGSFWLAKTITKPIDQMVRFSRSLGRGEWDT
                     EAPKHVKGELASLATAMEQMRTDIQGRHKEKEELLAQIAHEIRNPLGGIELMAGLVYE
                     DMPPESQQSAYIRQIRKEVSGLKNLVHQYLEFGRPAQAVGQLIDIRSLCVEVEQLLLN
                     EIKNKKIEINYSLSVPMIFFDPQHFRQIMLNLFANAVEALEMFGKIEVESKIHDSYIH
                     VSIRDNGKGIDEKWLSQVFEPFFTRSGHGNGLGLAICKKLCTANGATIHIENRPESGC
                     SVTMRLKQTI"
     gene            complement(32484..32984)
                     /locus_tag="PK937_01690"
     CDS             complement(32484..32984)
                     /locus_tag="PK937_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31317.1"
                     /translation="MQITRAGILSILFIAVVSCTEKDSGLFYETSFESINDLKAWKNV
                     NESQRINKAAPGAGEYSMAVGAGCIQPAAVFEKQVVASGFYRLRMWARMEENSASNAL
                     FSLTASDAENLSRIDLSVESGEWKLYESEEFEMRHGENFKLEVFCGGITFSQVNIDGL
                     QILQVR"
     gene            complement(33056..33871)
                     /locus_tag="PK937_01695"
     CDS             complement(33056..33871)
                     /locus_tag="PK937_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31318.1"
                     /translation="MSREELNDIVETQGRAMLSNSLVQLNNASYLAQREIADLPGLFI
                     LSPWGFRFEGESSMYDSLRYNHQTQYWSVRFERSNQSYELQYRYESPDQEGNPTSKSD
                     VMYLEFNYKNNHPIGSGAKAYDVEGTAKYTIKGIKNYSDPGKVGELYYSGSTISYFQY
                     QFSLDTVVKFTYRDIVQNVVLSEQSCVPNSGYFECTMTQDAVPGTFTIEPEIERVFYQ
                     SKLKDVPNQTFKDYEFRGKTLFTKEGIHILTDGEDFEISMPCDSIQNLIDTGH"
     gene            complement(33971..34360)
                     /locus_tag="PK937_01700"
     CDS             complement(33971..34360)
                     /locus_tag="PK937_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31319.1"
                     /translation="MHHKAIMLLCVITVSLFGCDSKKESGIDGTQWQTVSVMRLKEER
                     VHFENGTYHYWIKLHPSGCYEYAYYPYSETEKMKGKICTPIKMPDDICLGSFEVSNDT
                     LYVRFGEVVTAWTQVEFDDDQLLICGE"
     gene            complement(34381..34674)
                     /locus_tag="PK937_01705"
     CDS             complement(34381..34674)
                     /locus_tag="PK937_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31320.1"
                     /translation="MACSEGANIGTSENDKALFGSDEKISGVGYIKYVTVEGGYFELI
                     TSDNKIFDVHDLPDEYKKDGIVVKFTGHINKELVCVHAAAPILTLKIIERLEH"
     gene            complement(34952..35812)
                     /locus_tag="PK937_01710"
     CDS             complement(34952..35812)
                     /locus_tag="PK937_01710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNH31321.1"
                     /translation="METQEQINYKRIAEAIEYIRVNFKEQPDLDDVAKKVHVSPFHFQ
                     RLFTAWAGVSPKKFLQFTTIDYAKSILKDRKISLMDAAMESGLSGTSRLHDLFVNIEG
                     MTPGEYKNGGENLEINYSFSESPFGDILSASTSKGLCYMMFSDDPKSALSQLVSHFPN
                     ATYHHKKDSFHKNALNIFNVVAPENHSINLHLKGSEFQLKVWEALLKIPFGKLTTYNT
                     IAKEIRKPKASRAVGTAIGSNPVAYLIPCHRVIQSSGNFGGYMWGPTRKTAIIGWEAA
                     KISAQHEIVL"
     gene            complement(35909..36745)
                     /gene="ygiD"
                     /locus_tag="PK937_01715"
     CDS             complement(35909..36745)
                     /gene="ygiD"
                     /locus_tag="PK937_01715"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNH31322.1"
                     /translation="MRLREFSSITKSLPNTESMPILFLGHGSPMNAIEENEFVRGWRR
                     IGELLPRPKAILCISAHWETRGTYVTAMDKPKTIHDFGGFPKSLYEVEYPAPGSPDLA
                     LETQSIITKTKVGLDHGQWGLDHGCWSVVKHLYPNADIPIIQMSLDHYQHAEYHYELA
                     KEFHVLRQKGILIVGSGNMVHNLRMVAWDKLNDTAYGYDWAIEANETMKQKILTNDHS
                     ALIHYDKMGRAFDMAIPTPDHFLPLLYILALQKPDEKISFFNDKPVAGSLTMTSVLIH
                     NE"
     gene            complement(36935..38185)
                     /gene="pepT"
                     /locus_tag="PK937_01720"
     CDS             complement(36935..38185)
                     /gene="pepT"
                     /locus_tag="PK937_01720"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNH31323.1"
                     /translation="MFDPKYTFTCVDRFLKYVKYDTQSDEESTSFPSTEKQKNLSRDL
                     AAELKAMGLADAHMDEYGYVMATLPGNTSKNVDPIAFIAHVDTSPAVTGAGVNPVINK
                     NYQGGDIRLKNDSNQIIEVKNNPDLEKMIGFDIITTDGTTLLGADNKAGVAEIMDAVN
                     YFLSHPEVKHGPIKVCFTPDEEVGRGTEKIDLKKLGAKYAYTVDGSSRGEIETETFSA
                     DGMTIRFHGKNIHPGYAKGKMVNAIKVASHFIENLPKVGLSPETTEKREGFVHPTSFT
                     ANEELATIRFIIRDFVTAKLKEKEDFLKDLAEKTAAQFPGARVECEVKEQYRNMKEVL
                     DHHPQVGAYAVEAMKRLGIEPIQTPIRGGTDGSRLSFMGLPCPNIFAGEHSFHSKLEW
                     VAVQDMEAAVKTIIEIAQIWEEKA"
     gene            38366..40471
                     /gene="glgP"
                     /locus_tag="PK937_01725"
     CDS             38366..40471
                     /gene="glgP"
                     /locus_tag="PK937_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNH31324.1"
                     /translation="MDQRLNDLTDLAYNLWWSWNQNAQSVFRELSPQIWEESNHNAVA
                     VLHGTSHQELRARLNDSHFREKVDRVITQFRSYMRHENTWGSHHAQTFRDKPVAYFSP
                     EFALHECLPIYSGGLGVLSGDHIKSASDLDIPFVGVGLFYRQGYFQQHIAIDGTQQEL
                     YPLNDTTHLPLSLLSDRTGKPVVTVVEIGHSLVSLYCWQVKVGRAQLYLLDSNHPDNE
                     EHYRELTSRTYGGDIMTRICQEIVMGIGGVRLLRSIGIRPSVYHMNEGHSAFLTLELL
                     RERMLEGQPMDTARAWVREHCVFTTHTPVPAGHDRFSRDLMTFALANYCAILGLTIDQ
                     VMGLGRVHPDDEKETFCMTVLALKMSRASNAVSALHGNVSRDMWKELFPDRPVEQVPI
                     SHITNGIHILSWMNKRTRNFWRKFLGENWEQHIKQPDFWQRVADPSFISDEEIWALRY
                     DLRRMLIEFMRVRLKEQQRRAGGDGVRVFSNFFSPDILTIGFARRFATYKRAPMIVQN
                     LERAIQFFNDQDQPIQVVFSGKAHPKDDSGKAFIKRIVELSKHPQLFGKLAFIENYDL
                     YVARFLTSGCDVWLNNPRRPLEASGTSGQKIGIHGGLNLSILDGWWREGYDGTNGFAI
                     GKDESLGNEAEQDRLDEENLYLTLKNEVIPAFYNRDANGIPRVWIQKIRRAMQTLIPV
                     FNTDRMVAEYFETMYRRNS"
     gene            complement(40466..40927)
                     /locus_tag="PK937_01730"
     CDS             complement(40466..40927)
                     /locus_tag="PK937_01730"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNH31325.1"
                     /translation="MLKKNAIISELTWRDSVGQEHTLAQYSRHKAVVFFFYPMDFTRV
                     CTGQACYFRDVYGEIAAQGGVVFGVSLDDDATHEKFRTHHRLPYALIYDYDRTLSKKF
                     DTLWLWGLWRNKRVTYIIRPSDGKIVDVIHAERDAQVHADRVLAYLRASSL"
     gene            complement(40935..42254)
                     /gene="hpnE"
                     /locus_tag="PK937_01735"
     CDS             complement(40935..42254)
                     /gene="hpnE"
                     /locus_tag="PK937_01735"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNH31326.1"
                     /translation="MAKSPSQIIIIGAGTAGLAAATTLAQAGYDVSLYEKNAYAGGRT
                     FSFTDVASRSTLDNGQHLLMGCYHATRAWLDSLNASLDIPKKSFPLNFYSADGRIACL
                     SLPDWPAPWYALYGIMRYDAIPLSERWRILRAGLSMRKSLIRGRYVSDWLDAWRFTQD
                     TRRWFWNPICLAIMNQHPDDADLVQFGEAMRRIFFGSRDDGRFIISEKSLDAVLVEPA
                     VRFLKQKSCSLHFHSFVRHITVSNGRVEGITLRNGETIRTDIVIMAVPPWSAGKLLPV
                     ENEWTSRLQNFSSAPILSAYIWLNEMHPDALFDGPMAACIGTHVQWVFKKNSECIQIT
                     ISHPGASVFDAASIWEKRITDDMTLLFKNFKPSSVRRIEMIKSVRATSNNHAKDSYRF
                     HSRTGIQGLYLAGDWIADELPSTIESAVLSGIRCAEAVLQDEKNKRS"
     gene            complement(42247..43074)
                     /locus_tag="PK937_01740"
     CDS             complement(42247..43074)
                     /locus_tag="PK937_01740"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNH31327.1"
                     /translation="MDIETILQEDARSITRRSRSSFYYPMMLFPGGKREAIFTVYAFC
                     RITDDIVDQNGSDAATHLSVWKKELIAAERGYSSYYLLNRVMRVARDFSIPVQLFHDV
                     IRGMEMDLERREYRTFDELYEYCYCVASAVGLMIIRILGAHHTASERFALHAGLALQL
                     TNTIRDAASDIADGRIYFPVTDRRAFFDNPSAVQDALRLQCAEARKQFQLADEAFRAY
                     PSLALFPARVMENMYRLLLDKIEQTGFTPTAHRVSLTLSEKIQTAGRTWFDDRPYRG"
     gene            complement(43065..43958)
                     /gene="hpnC"
                     /locus_tag="PK937_01745"
     CDS             complement(43065..43958)
                     /gene="hpnC"
                     /locus_tag="PK937_01745"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNH31328.1"
                     /translation="MNEERAIGSYSVDEIDAAYAMCRDFAASHYENFPVISWIFNAAQ
                     RRHLAAVYTFLRRADDVADEGTGTIEQRMHGLLWMQAALKASEEGRAKDPMFAALSVT
                     LQKGGIPRQWLDDVLSAFKVDLLKNRYTDEEQLMGYCRYSANPVGLIVLHILGYKYNS
                     TMWEKMTPLSDAICSALQLTNHWQDVFIDLQKNRLYIPLELMKECGYTEEDWKNKTVN
                     PSFSKTMAVCLKKTRDLFDQGHALLGYLRFKDRKIIGMIWLSGLRVLMKMEKTGGDTL
                     NHRPTIGLTDKFWILWRVLWI"
     gene            complement(43951..44445)
                     /locus_tag="PK937_01750"
     CDS             complement(43951..44445)
                     /locus_tag="PK937_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNH31329.1"
                     /translation="MNIRNYTWRGAILLFAGAWIGILVFLAYGVAGTIFRNLDSKTIA
                     GYINGLILVRMNHLEYLCAALIVVSSFVLYRRQKTLWSRVRGVVSGVMLINLFFYADF
                     ITPQMTMLKEKIVDFDQYAKENDPRPERKEFDAWHRRYSALVGFNIVLALWVVGLTLR
                     ERDE"
     gene            complement(44442..45578)
                     /gene="thiO"
                     /locus_tag="PK937_01755"
     CDS             complement(44442..45578)
                     /gene="thiO"
                     /locus_tag="PK937_01755"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNH31330.1"
                     /translation="MKIYDVVVIGGGVIGLSIAYEIKFRHNKIKVAILEKTQTGYEAS
                     HASAGMLEPQLIMPRQNLSDREKAFFRLCRESQNMYEETIRRIEYHSRMDCEYRKEGI
                     LKLISPETDPKPWLSWYVGLGMHAHFWSVEQTGEAEPALREGMAAIHLPDNHQVENRK
                     LSAALAECCKNLGVDILEYSAVESIILNNEAIDHLQTKTGTVSAARYVLAAGAWSSQI
                     PGLTQTIPEIKPIRGQIVAMQMPKPDFIRYPAYLEDFYYVPRNSGRLLIGSTVEEAGF
                     DKAVTPSVIVGFIEKLRALIPQSKTFESVERWAGLRPCSADRLPLLGETRLTNLIVAT
                     GHFRNGILLMPITAKLIADMIITGKSSPMLAPFSPSRFNGSTNA"
     gene            45792..48002
                     /locus_tag="PK937_01760"
     CDS             45792..48002
                     /locus_tag="PK937_01760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH31331.1"
                     /translation="MEPKKLGRYEIVQVLGQGAMGIVYKGVDPMIGRTVALKTLKSGS
                     EIPEQQLTEFKRRFAQEAQSAGRLNHRNIVTVYDVGEEEGLAYIAMEFIKGRPLDDLI
                     AEKHPFTIEQIVDIMIQICEGLGYAHKNGVIHRDIKPANIVLTDDQIAKVTDFGIAKL
                     TSTSATQTGMVVGTPSYMSPEQITGRGVDNRSDIFSIGAVFYELLTYEKAFPGDNITT
                     VMYRVVHENPAPLSISNAMVPPQFQAIIERAMAKNPADRYPDAETMASDIRNYKTATA
                     NMGATQVINPANYAGATVVMSAPDFSAMSPTGTGSMPSPAAAQTQSIPAASPASKKSP
                     VALIAGGAVAAILIVLYMMFGGSGDKSSASSAKSSGTGNASLTLTLNAAEGTAMLDSV
                     HVPIKDAKISAEKITAGEHELVIQSDGYETYRSKLLFADGETKQFAVELKLAPVKFPA
                     GVDTAYITVSSTDMIKVMTNTGTMLGYSPIQRLPFPSGKHTLVFSKPFYTNQVSEIDA
                     RKGKEIKLTPKLQAKRVKANLAGVEPKKIQVYLNSVASESQLKPEKDGVTYSLPLGTN
                     KLIVTADGYKSYEQELNLGDDAIPFDVKATLVQGFGSLAITSKPDGADVYVDGKKEGV
                     TPFKMDKIPAGPRDIKVQKGSLLSIKKMNIEADKENAADFTLAASTGILKILINPWGN
                     ISVDGKPMGASPPLERLELSPGNHTIKIENPAYKAVTKQVKITVGQTTTLQYDF"
     gene            48004..48723
                     /locus_tag="PK937_01765"
     CDS             48004..48723
                     /locus_tag="PK937_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31332.1"
                     /translation="MKKHFSLITLLFAGIIIFTGCGGSKNTTKEKPETPAVPKKPSGK
                     PQLEKGMELYKARDYEGAKKNLRSATDLDLSKSERTQTYKHLGFIFAMQKNQTEATKA
                     FFDAFTSDGEFELESAEMGNPAWTPAYEAAQKQFTLASASGSDLLSKGKDAYSKRNYD
                     EALSYLETAVKKTDLEGNKRADAYKLLAFAYALRKRPVDAKNAFRKAFEANKNFELDK
                     SEYGNPVWTPLYDEIKNTIKK"
     gene            48742..50436
                     /locus_tag="PK937_01770"
     CDS             48742..50436
                     /locus_tag="PK937_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH31333.1"
                     /translation="MASLVLIENNTTATRFRIEKDDTIIGRRPENDIMIANVMISGKH
                     ARVEKKNGLYQITDLGSTNGTFVNGARVTGSAEIKNQDHINFGAVELIFMTDENAMVN
                     IDRTRFYSRDKAVAPPQKPHDTGYAANPNADKIVQLFQQLKNTVLSGSADRTAIMTQF
                     MEVEHQLQIMNMQFKESEKSQQKLNVLYEIGKVINHILVEEELLKTIIDLALKVMNGD
                     CGFIMLYDDKNNLVPRVSRKMQADEISQSGSTFSSTIAKQVVESNQSILTSDAKSDSR
                     FQSGASIISNNIRSVICSPMRNKDQAVIGVIYVGSNVMTNVFSKSDVELLEAFANHAA
                     ISIENAKMHEERRRKEHLKSALERYVSKQIAERIMSNDGSSIRFTPERREVTLIFSDV
                     RGFTTLSEKLSAEEMVEILNRYFSRMIDVIFKYGGTLDKFIGDSIMALFGAPATSGDD
                     AWNAVQAAIEMQRGLEAFNDEQRQLGKPEIQVGIGINTGHVVVGNIGSDQRMEYTAIG
                     DNVNLASRLQGKAAGGKIIISKATYDFVKDKVQATLLGTTEVKGKTIPVEIYEVMY"
     gene            50488..51705
                     /locus_tag="PK937_01775"
     CDS             50488..51705
                     /locus_tag="PK937_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HNH31334.1"
                     /translation="MDLNTLIYDWNQEVNAEEKPAHAFELNDETLRDGLQSPSVKTPT
                     LEQKIEILHLMEGLGIHAADIGLPGAGPHVLEHVVALAAEIAKNKFKIQPNCAARTLK
                     VDIDPIIEASQRTGIPIQACTFIGSSPIRQYAEGWGMDQLLKFTEEAVSYAVKNNLDV
                     MYVTEDTTRAHPNDVKRLYLAAIRSGARRVCISDTVGNATPEGVQRLVRFMVEVIKES
                     GEDVKIDWHGHNDRGLAVANAIIALQAGAHRVHGTGIGVGERVGNVQMDQLMVNLKLK
                     NWITNDLTTLPQYCRKISEYTGIPIPINYPVFGHDAFRTGTGVHAAAILKSLNKGDME
                     LVDRVYSSVPAAMFGLEQVVEIGPLSGESNVVFWLKRRKIEPREELVKKIFAAAKQSQ
                     TVLSDEQIFSLCR"
     gene            51715..53397
                     /locus_tag="PK937_01780"
     CDS             51715..53397
                     /locus_tag="PK937_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH31335.1"
                     /translation="MILSRVSLFIFLISNTLWGQTTEKTYWIFFKDKPDYLHATVIPD
                     ISPRALERRLKQNPHTSALIDESDYPVSSDYLAALSSHLVLIHNTSRWLNAACVQINE
                     RNIPLIQSLPFIREIRPVRARSVIASTSSHASSFSSIAASFSRFSYGASLRQNAFVNA
                     VAAHDSGYSGKGVRIGMIDTGFNLTHTAFSGIHVVADSDFVDHDGNVSEIGSKFTSSH
                     GTFVLSAIASFHESFLIGTAFGADYILARTENNSDAIYELADESRWIAALEWMEQIGA
                     DIVSSSTSFFDEFPGTSNDYTIRDLDGKTGLTTLATDIAFKKGLLVFNSSGNNGAEDQ
                     DQYRVSTPSDGKHMIAVGATIGDSTVASFSSRGPTADGRKKPDVAALGYEIRVVSSGT
                     TGYQNFSGTSLSTPIVAGIAALALEANPAWSGSELYDAVRKTASLSKKPNHTIGYGVP
                     DAMKIIRYNPASSDDFNPIRDVVNYPNPANPSTTIRFRSVISGRYSVHIYNTLGALVR
                     TIRDNASITAGETVAALWDLKNDHGRRVSSGVYIYRIAMDDKSETRKILIIK"
     gene            complement(53387..54550)
                     /locus_tag="PK937_01785"
     CDS             complement(53387..54550)
                     /locus_tag="PK937_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNH31336.1"
                     /translation="MQKTKARIYLDHSATTPVDKRVVDAMLPFFTEEFGNPSSIYGLG
                     RSAKVAIEDAREKVASVIKAQPSEIVFTGGGSESDNMAIVGAVKSMSAKGKHIITTRT
                     EHHAVLHTFESLEKSGYAVSYVPCDQFGYVSAETIAQAIRPDTVFISVIHINNEVGTI
                     NPIEEISALAKSHQILFHTDAVQSFGKIPFDVKNLPVDLLSVSAHKIYGPKGVGCLYI
                     RRGVKIPSFIEGGSQERGQRAGTENVPGIVGFGKAAEICKDSMSGESERLKKLMTVFW
                     KTLQDIAPQARLNGHPEKRLPGHFNISFPGIGGDEIAMMLDLEGISVSTGSACTSGAV
                     SGSHVLKAMGLGPEFYGSAVRFTFGRLNSEQDIPSVMKALQRIVEKNKNASIL"
     gene            complement(54552..55688)
                     /gene="alr"
                     /locus_tag="PK937_01790"
     CDS             complement(54552..55688)
                     /gene="alr"
                     /locus_tag="PK937_01790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNH31337.1"
                     /translation="MDTIYHNILKEIRPTHAVIDLAAIAHNMAQIRTRVGSRKIMAIV
                     KANAYGHGICEVTQKAVRSGADYIGVGFLEEALFLRQEDIEAPILVMGGILGYQVKKF
                     VQNDLEITVSSLELAHHINDEIKNQKARVHLKIDTGMERIGVTYKQAVEFVRRVAMLP
                     HIEIVGLYSHFATADEENLFFAHTQFERYCEIIDALKKLGIEIPIKHIANSGAVMNLD
                     STYVDMVRPGISLYGMYPSRFVRRDIQLQPALSLKSKVVFIKRVPAGTGISYGHKYIT
                     PQDCNIVTVPIGYGDGYTRLLTNKADVLIRGKRYPVVGAVCMDQLMVNIGDDRVEIGD
                     EVILIGRSGNEEITAMELAEKIGSIPYEITCMINARVPRIHIHK"
     gene            complement(55692..56516)
                     /locus_tag="PK937_01795"
     CDS             complement(55692..56516)
                     /locus_tag="PK937_01795"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNH31338.1"
                     /translation="MHELKKMMEESIAIIRSKTTMRPTIGIILGSGLGDLADRFENKT
                     VISTADIPHYAKSTVEGHDGKLVFGTLNGKHVLAVKGRVHYYEGYTMAQVTYVVRLMG
                     MLGIRSLIVTNAAGGVNPLFQPGDLMFITSHINFFFANPLQGPNDETIGPRFPDMESE
                     YDREYISIAEKTALKLGVRTQRGVYCAGSGPTYETRAEVKMFQKIGADAVGMSTVPEV
                     IVAKHQNMRVLGISCITNLGTGISQTKLSHEEVTETANRVKTQFQQLITDVVGQIG"
     gene            complement(56567..57163)
                     /gene="recR"
                     /locus_tag="PK937_01800"
     CDS             complement(56567..57163)
                     /gene="recR"
                     /locus_tag="PK937_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNH31339.1"
                     /translation="MQYTSETIERLIKEFSKLPGIGRKTAQRLVFHLLKQPKDEVVML
                     SQALLDMKEKIGRCTTCFNVTEHNPCAICANPRRDHGVICVVEEANDVLAIERTDMFR
                     GVYHVLGGLLNPLEGVGPDDLRIRELIQRSENEVREMILAINPTVPGDATVMFVAEKM
                     QGKSVRLTRIARGIPIGSDLEFADMTTLTRALEGRVSV"
     gene            complement(57170..57496)
                     /locus_tag="PK937_01805"
     CDS             complement(57170..57496)
                     /locus_tag="PK937_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNH31340.1"
                     /translation="MRGFDMQSLMKEAQKMQKQLESRQSELEQVNVEGTSGGGMVTVT
                     VNGNGKVLSVKIQKEAVNPDDVGLLEDMILSAIHNAVQKADEMSNAEISKITGGMMPG
                     MKMPKK"
     gene            complement(57514..57978)
                     /locus_tag="PK937_01810"
     CDS             complement(57514..57978)
                     /locus_tag="PK937_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31341.1"
                     /translation="MELAWPYELQSDLLKLGFTTSGVTPFQVEMVQKEREFVAGVIAQ
                     FTAKRVRLVLEPEKIIRPVERVAYQDEPVPEPSPAENFFDHYPTLDAAQEPFTEADVK
                     PDGNEAQISVQVRSDLFKYVRDKEAEMPLKRMIELLDCELIDITNKDTLTTS"
     gene            complement(58173..59339)
                     /gene="dnaX"
                     /locus_tag="PK937_01815"
     CDS             complement(58173..59339)
                     /gene="dnaX"
                     /locus_tag="PK937_01815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNH31342.1"
                     /translation="MSYLVIARKYRPMVFDDVIGQEHITTTLKNAIESKRIAHAYIFS
                     GPRGVGKTTSARIFAKAINCENGGPTITPCNQCQSCLEITGGRSLDVLEIDGASNRGI
                     DEIRSIRDNVRYLPVRGGYKIYIIDEFHMITKDAFNALLKTLEEPPPQVVFIFATTEP
                     HKILPTILSRCQRFDFKRVAIEKIIGRLKHICRIEQIDMDEESLFEIAQMADGGMRDA
                     LTLLDQVISFCGNQVRIQRVREALGLIETGLYFELMAIIRNKSVSEGFKFASRVLDAG
                     LDVTEFLHGLMAHIRNVLLAKACGASALTTVYESYHEKFTDVSQQFSETDLIRMMNLV
                     SETIQRVKWSLKQRFVFELMLMKLIRMEDSVQLSEIIRKLENPDTETVKKKVVP"
     gene            complement(59505..59603)
                     /gene="ffs"
                     /locus_tag="PK937_01820"
     ncRNA           complement(59505..59603)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK937_01820"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(59699..59782)
                     /locus_tag="PK937_01825"
     tRNA            complement(59699..59782)
                     /locus_tag="PK937_01825"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59746..59748),aa:Tyr,seq:gta)
     gene            complement(59807..59879)
                     /locus_tag="PK937_01830"
     tRNA            complement(59807..59879)
                     /locus_tag="PK937_01830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59844..59846),aa:Thr,seq:tgt)
     gene            60082..61359
                     /locus_tag="PK937_01835"
     CDS             60082..61359
                     /locus_tag="PK937_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNH31343.1"
                     /translation="MSEISRIEILLFFLLIFIGGFFSSFETAILSLDKLSFKKILKRG
                     GTRSKLLELWTSNQNDFLTGIYVGNNAFNVGAAVLAASVSVDIARLYGYREAYALAVA
                     TFLITLFLLLFSEVLPKSIARHNPEKTVMLLATPLHIALLILKPFSGFFSWVSRVVVT
                     ATGGETHRRSLNVTEDEIREIINAGEVAGAIEHNEREMIHSIIEFGDTLVKEVMVPRV
                     DMVCVEVQTPMEEILQIMADEKLSRIPVYEENMDTVVGVLHIKNVMNFWRKNIQDMTA
                     IEFITMPYFVPETKKISELLREFQSQRIQMAIVVDEYGGTAGLVTIEDLIEEIVGEIK
                     DEYDDNVSLIKKQEDGSYLADAKIEIYLINEQLHLHLPSDEYSTLSGLILWLFKRMPK
                     KGDTISYDDVRFTIIESDRKRIHKVLIQIPSPP"
     gene            61450..62502
                     /gene="corA"
                     /locus_tag="PK937_01840"
     CDS             61450..62502
                     /gene="corA"
                     /locus_tag="PK937_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNH31344.1"
                     /translation="MITIYAQLFGKEFVNTLTFDELVIYLKDPQCIFWIDLNSPTLDE
                     IHQIQDAFNFHPLCIEDVMSYSNSPKLDEFDEYIFLVTHEPRMHPETNEIERPEIDFF
                     LGKNYLVSVHHEPSPAIAKSIHRCETQLLFHQAALYEKGSRGRTTIKDNFMFKNSDFI
                     LHTILDHIVDDYFPLVEKWEDDIDQLEEHVLSVRAERSVLNEILKLKRQLAGFRRTVS
                     PQRDVLSRLIHLQHPAMSKASVVYFRDIFDHLIRVNELIDTYRDTMSNVLDAYYSVLS
                     HQINENSHIINIIMKRLTIITTIFMPLTFIAGIYGMNFHNMPEIRWEYGYYYALGLMG
                     ALALSMFYFFRKKFWF"
     gene            62519..>64110
                     /gene="purL"
                     /locus_tag="PK937_01845"
     CDS             62519..>64110
                     /gene="purL"
                     /locus_tag="PK937_01845"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNH31345.1"
                     /translation="MHTTKAIELHEIREPEVTHKLAKDHGLTDEEYERIQQILGRKPT
                     FTELGIFSVMWSEHCSYKNSILILKTLPKQGQFILKGAGEENAGLIDIGDGLAVAFKI
                     ESHNHPSAIEPFQGAATGVGGILRDIFTMGARPIAALNSLRFGHLDDERVRYLFANVV
                     KGIGHYGNCFGVPTVGGEVYFEECYTDNPLVNAMAVGIVRHDQTASAIAKGEGNPVMI
                     VGSATGRDGIHGATFASEEISEASEAKRPNVQIGDPFTEKLLLEATLEAIQSGHLVGI
                     QDMGAAGLTCSSSETSAKGESGIEINLELVPTREDKMTPYEIMLSESQERMLLIVKKG
                     YEKEIQNIFEKWDLHAVTIGHVTNDKLMRVKYKGRVFAEIPADTLVLGGGAPVYKRES
                     AEPAYLKETRALDIASLPQPSDLTSVLTRLLSTPNIASKEWVYEQYDSMVRTNTVTLP
                     GSDSAVVRIKGTNKALAMNVDCNGRYVYLNPRRGGHIAVAESARNVVCSGGRPAAITN
                     CLNFGNPYKPENYWQFKEAVEG"
BASE COUNT        17875 a        14918 c        13156 g        18161 t
ORIGIN      
        1 tgacactctg attactcacg tgatccgtat tatcaccttc accttgcacg aaggtatggg
       61 taacacctaa attgaaagta tgttgcttta gaagagttat cagattagtc caacgtgtat
      121 ggtaactatt agaaatattc ctactatgga taaccgtcgg aaatgaattg gtataatcaa
      181 actgatattt ataccaactc gagcctatcg ccgtaagcca ttgctcgtta aagcggtgtt
      241 gtatctgcgg cgcgatgaga aatacacgat cttcctgatt tccgcgcgat gttacagatg
      301 tattattact aatcgtaccg ggtatctcca catcacgaaa cgtccattga ctcatcaaag
      361 aaaaatccgt ttttaagctt aatctgttat taacattgag aaataaagca tgacgtttgc
      421 tttccgaatt cacccgcggt acacgttgtc ctgaggacgg cacggtaaca aaataactgc
      481 tatcgggttg gtattcgaac tcataggcga cactataaga cgtttgcttt atgaaacctg
      541 ttagccccgc tgataaccat cgtcttttaa atgaactata gtgcgctttc gttataattg
      601 acatcggttc atcacgtggt tgtttttttg ttataaggtt tattgtccca cctacggcgt
      661 tattgtcgcc acctttgtat gtttcgatat gatcaatcat ggtcaaagga acaacaccca
      721 aatcaattaa gcccgtagcc ggattattca gcggtattcc gtcaatcatg atgataacct
      781 gctcagaagt agagccacgg aaagatggaa gtttgagtcc tgaagttccg ccgaagtctt
      841 taatttgtat ccctgaagcg ccactcaaag cgtcggacag gtttgtagcc cctcgccttt
      901 ggatattttc ctctgttatg acattaacca atgcagaaga ggcatttctg gaatttatca
      961 aacgtgatga ggtaataacc gtaccctgca agtcatattg cttaatttta acactatccc
     1021 cggtttgcga ataatattcg gcaatggcga accacaataa tccgatgtat atggtacttt
     1081 tcatcatatc aaaaaaaaat cctcattcgg aaagaatgag gataatagaa aagttgactt
     1141 tattgatccc cacgccttcc cctcgaaggt tggtcgaatg cagtttaggc aggtctcctg
     1201 acttgcgcgt taaagattgc cttcccatta ccgataaatt cgataacagt ggacttatta
     1261 gaatgcgctt acagttgcgg ggcagctcat gattttcaca tgattccctt ttcatcacgt
     1321 cgtatgcgtg acacctaaac taaatagcaa agaacaattg acagttctca agataaccga
     1381 aaacttttct ttgtcaagtt ttaaaataat tacttccaaa tcacccacga tgcccaataa
     1441 aaaggaagcg tgttttgaac gataaaatcg cgtttggttt tttgtagtgc cgccggaatg
     1501 gatttttcga taacccaatg ctgatacaac tgatcaacaa aatttactgc atcgcgatcg
     1561 ttaattttcc ataaggtact taaaactaac gaggcaccgg aattaagaaa cgcttcaggt
     1621 atagccggga tatttttctg tgatatttta gtgtttacgg atgtttcaca agccgataaa
     1681 ataaccgatt gacctcttaa ttgttgtttt tcgatttcat tacacgtcaa atatccatca
     1741 tggtgaattt gatcacgccc taatataatg ccgcttttac gggcatcgtc tgtcgcattc
     1801 ataccatgac ctatcaaatg caaaacttgt gatgaatccg tgccaatgat atcataatag
     1861 cttttttcag taacttggta ataaatttgg ctatgcggaa taatagactt gatgcgatct
     1921 atttcgttat ctacatccgg cagatccggc aatgacgtcc acgaactata ttccgagcgg
     1981 gccaatatag tgaccgacgg tattgttcgt tccgaataga tcgaaaattt ttcatcagaa
     2041 tttagcgctg ctgtatattg gatcgtatgc gtttcagtca gatatcggcg ttctaagctc
     2101 aacatctcaa aaggaaaatt acacaacaaa ccgtcaggaa ctatgaccaa ttctttatgt
     2161 gattgaacgt aatctataat cgctggattt atccaatccg ataatacagc aagatgctgt
     2221 gatagactat gctgattagc ggaaggttgt cgaagaaggg ttttaatttg taaaaggtga
     2281 tccttaataa gtggtgttat acacaaacga cgatattgca gttccctctc ggtattgagt
     2341 aaaacatgca tgactgtatc agcttgaaag aatgaaacgc cggattcaaa aaaaagaatt
     2401 ggatgcggtt tagtattatg agtagagcga ctgtttaata aaagctgata gctcaatgct
     2461 tcatgatact ttttttgatc aaaaagaaat cgaatatagt ttttataaaa tcgagtacga
     2521 tcattgggaa tactggcatc atatacatgt gcagaatttt gttcctgata gatcgaatct
     2581 atggattgca gcgcaaggac ataatatgca tcagcactat ccgtttggtt aattgattca
     2641 aagtataaag cacgcgcgtc ataataactt gggctatacc gttttgaagg aagttgggac
     2701 aacaaattga aagcttgttc taacaattga tccccttgtt taaattgttt taacagcacc
     2761 atcgcacgaa atttttcaag aagggtgaaa aatgtctgat ctatcgaacc aatttcacaa
     2821 taaatatcat atgctttatt ccaaaagtat atagcgctgt catattgcga taacgcgcga
     2881 aatgccccgg ctaattcata ataagcgtca gccgttgcaa ttttgtcacc tgctttttca
     2941 gtgataacca atgagcttcg taaacattga agggctgatt ttatttggcc atggttatta
     3001 aataaatgtg ccatattgtt caaaattatt ccctcggttc ctacatctcc ggtcattcgt
     3061 atgtattcaa gtgctttttc aaaacaataa cggctgtcag catataattg catctctgaa
     3121 aaaatcaaac tcaagttttg ataatcagcc gccatgtcgg ccttatcatt acgcagagaa
     3181 tcttggtaca aagccattct atatttttct attgcggaag caaaatcccc catcatttta
     3241 taaacaatgc cacgattgga aacaatccac gattgcaatt tgagatctaa gtcgcggtgc
     3301 atgtaatgct cagctttatt caaagcttca ttggctaaaa agggttgaga agattggata
     3361 tagatgatac caaccgtatt ccaatagcgg gccaaggcgg tcgaatcttt aaccgtagga
     3421 agtattgatt ggattatagt gagtaacgag tcgcagagtt taaaattacc tgtttcagca
     3481 gctacgagaa tataatcctg agtccaccga aaacgatcca ttgttgctac atccttacgt
     3541 agtaacagcg atgaatacaa actttgtgac tccttgtttt gaccaagata gaaatacgct
     3601 ttcgacagcg cagcatgggt agcgggctta tgtattgtgg aaatactatt tacttcaaaa
     3661 aggtgtatgg catcggcata cttactttca cgaagtaagg cgtccacact atcaagtaat
     3721 atatgatcct gagcatttat ttggctcata acaaacagaa accaaagaaa caatctcata
     3781 aaaatcattt cagcttaatt tcaaaagcat ccttcaacga gtgataatta actgtaggcg
     3841 tatatttttc ttttaagctt aataaccatt tatcacgtgt ctgtttgatt aatgaaactg
     3901 aataatcaga tttaacttga acaaacactt tatccaatga ataccagctc ggttgtgttc
     3961 ttttgtaaat cttaactata ccgtaatggc catcgttcat aataaagacg tcacttatat
     4021 caccttcttt gagttcttgt atacgcgctg acaagttccc aaattccttt aaaggtctca
     4081 gaccggaacg cacagcagat gccgggtttt tctttcgcaa ttcttcaaaa cttatttcaa
     4141 agttccttgt agatttaatt ttatctgcga taggccgtat ctgcgcttct tcgttcatta
     4201 caatcattcc caaatccaaa ctgtccggca ttttgtaatt ctttatattt gcctcataat
     4261 agtgacgaac attagatgta tcttcgatca attgaatatc aactgaaggc ggttgaactt
     4321 ttgtagagat caatccgctt tctattcgct tgataaaacg tttttcgcga tcatttagtt
     4381 cgattttctg ttctttagcc caacgtgata caagtctaac agctatggca ttgcgaatac
     4441 cggactccat ctgttctatc gtaatacgtg aattaaagcg attgccggtg agtgcgaagg
     4501 ctatatccac tatttttatt gtatccgtca gtgatgtagc gaggatcgta tttagttcat
     4561 catcggttaa ctgatcaacc aatggttttt tggaatcata ttttttgaat ttagaatacc
     4621 atgccgcagc attggattga ttaaaactgt atgacccgtc acgtagccga ttttcgatga
     4681 actctttttt atttttttcg atttgtatat caggttttaa aagaatttgt ttagcaattt
     4741 gctccttaat ggcaatcttt gctttttcat atggcaacac attagcatca ttgctttttt
     4801 gtatgatttt ataaatatga taaccgttcc cgtcgtcaat cggttgagat aattcatcgg
     4861 gttgcagttt gaacaaagca tcgcctattt cattaggcaa ttcacctcga cgtaagcctt
     4921 tgatagtgcc atcgaaaaat tttgtagctg catcgtcaga aaactgttct ataaaatttt
     4981 caaatttatc gggttctttt ttgagtacgc ccaacaaaga atcagcaatg gatttggcgg
     5041 ccagcttaaa tcgtatgatt ttcttttgat tgggtgccat ttccaaagaa ggatcaaaag
     5101 ttttatcata tccgataaag atatgtttta catccaactg ctcttttagc tgttcatatc
     5161 gcgctttaat ttgatcttct gttattgagg gttctacaat tgcatttcgt atcgcatgag
     5221 caaaataaaa ctcgtcttcc ttagtacgaa cttgaaaacg cacataagga tgatcctgta
     5281 atttctgtga tctcccctcc atgaccacaa ggtaatagtt taccatctca tcaaggattt
     5341 gacgtcgaat aatttccgac tctaaatccg gtttatcctg tttcaattct gaaacccgat
     5401 ttttataatc acctaaaaaa acggtatctt ctccgactac cgctaatact gttctcaaat
     5461 cattcaaatc ggtttttttt tcatcatttt tacaggctat ggtcataacc gtaatcaata
     5521 gcacccacga gtacatttga attttagaaa aatacatatt taaccttatg aatgatttat
     5581 aactcaatta tttgtgaaat tatttttttt gcacttatca atactaaaga atcttcttta
     5641 gactcagttg aaataatacg gctttttgtc gcatcatacc actcaaaacc aaaagtaata
     5701 tctaaaaagt ccttattctg aaaaaatcga agattttcga atttttttac aatagaattc
     5761 atttttaatt tcatgcgatc tgccacattc ggttgactca tgcgtttcac atcaaaccga
     5821 agtactaatt tgttttcatc aatgtccaat cgttcagcac aaagcggcaa tgtcatcaac
     5881 tttatttcga tcatttgcag aagattttta gcttctactg gaagcttccc aaaacggtcc
     5941 accaattctt gttcgattgc atttaaagca tcaattgaaa tgatatccga taagctttta
     6001 taaatcctga ccctttcaga tggaacatta acataagtat ccggtatata ggtatcgcaa
     6061 aaaacatgaa cctgtaaagc cggaatatac tctgcttgct gagttatttc ctctttttta
     6121 cccgactcga ccacattcat ttcttggcgt gtctcttgta cggcttcatc caaaatttga
     6181 caataaagtt caaaaccaac cgcatttaca aaaccgcttt gttcagcgcc aagcatattg
     6241 ccggcaccgc gaatttctaa gtcccgcata gcaatcaaaa aacctgaacc cagatccgag
     6301 aattcctcta tcgcttgcaa tcgtcgcaac gcatccgaag gcattacact catgggcatc
     6361 gtgagaagat agcaatacgc ctgatgattg gagcgaccga cccgaccgcg taattgatat
     6421 aactgcgata acccaaactg atgtgcctgg tctatgaaga tagtattgac gtttggtatg
     6481 tcaataccgt tttcaatgat tgtcgtagca actaaaatat catattcgcg tttcatgaaa
     6541 gcatgaacga tatcctcaac ttgttgcggt gccatttgcc cgtggatcac agctaatcgt
     6601 gcttttggaa caaccgtatt gagcatgccc gtgatgcgat caatactctg tatccgattg
     6661 tgcacataat aaacctgtcc accccggtcc aattctttca tcacggcatc atggatgagg
     6721 tcttcgtcaa actgcgttat ctctgtttta acgggtaaac gatcctgcgg tggcgtattg
     6781 atgaccgaca agtcacgtcc gcccatcaac gaaaagtgca atgttcgcgg tatcggcgtc
     6841 gctgtcagag tgatcgtatc gacggtagct ttgagctggc gaagtttttc cttatgagca
     6901 acgccgaagc gctgttcctc atcaataata agaaggccta aatctttgaa ctgcacatcc
     6961 tttgaaagaa gtcggtgcgt gccgatcagt atatcaattt cgccgcgctg tactttttcg
     7021 aggatgattt tttgttcttt ggttgtccga aaacgagata tcacatcaat gcgaaccgga
     7081 aattcgcgca tacgtgaaga aaacgtttca aaatgctgtt ccgccaaaat cgtcgtggga
     7141 acgagtatcg ccgcttgctt actatcctga acggctttaa aagcagcacg tatagcgatt
     7201 tccgttttac cgtaacccac atcaccacag acaagcctat ccatcggatt atcgctttcc
     7261 atgtcctttt tgacgtcgag cgtcaccttg gtttgatcgg gcgtatcttc aaattcgaac
     7321 gatgcttcca atgcgtattg catatgggta tccggagtaa aagcgaatcc tttgcgtgat
     7381 ttgcgttcgg catagagttt gatcagatcc tgtgcgatat ctttcagcgt ttttttggtg
     7441 cgtgctttaa ccttatccca atcgcctgaa cccaatttat gtaacgtcgg tgtaacgccg
     7501 tcttcagccg aaaacttttg gacgcgtgaa aaatgttcga gcgggacaaa taatttatca
     7561 ccgtttttat aaagaagttt caaacattct tcggtatgtt caccgctggt tactttttca
     7621 agaccggcat agcgcgcaat accgtaatcc acatgaacta cataatctcc cggtttgagc
     7681 gatgcaatgt gacgtaacgc ctgcgctgtt ttaaatttgc gatgtgatcg cgtacgcttg
     7741 atgcgtccaa aaatctgatg atccgtaaat acgacacatt gagcatcttc cgtgtaaaat
     7801 ccttcatgca actcgccgac gccgattaca aaatcaacgc cggacgcaag ttcatcggaa
     7861 tccaacaatt cggataatcg ttcagcctga cccgtattat tacacgctat gaaaacctta
     7921 tattggcccc gaatggctat caatcgttct ttgagcaggt tgatgtgact gttaaatgat
     7981 tcgagtgcat gagccgtaaa atttatttta tcaaaatcca taatacggct ataacgggca
     8041 aaataaagac acgcaaaggc attgagtttt ttcattacgg acgcggtatc aagaaaacga
     8101 ctttccgggg ctagaagttc tgaatgagct tcggatttac gctgataact atcgttgatt
     8161 tcatcccaat acttatccaa tgcgccttgc aaaatttctg gttctaataa catgaccagt
     8221 gtgtcctgtg gccaataatc cgtcagatcg gcatcgtgtt gtgccgtttg actttccgat
     8281 ttgggataca gagctaaata atctgttttt tgcaacgaac gctgatttac tacatcaaaa
     8341 aaacgaagcg aatcaatcgt atcgccgaaa aattcgatac ggataggatt ttgttgagca
     8401 aaaggaaata cgtcaacgat accaccacga atggcaaacg ttcccggctg atccacgatt
     8461 tcttgctttt cgaaaccgat ctcatggagc aattcgacca agtattgaaa ctgaaacgtg
     8521 gcgccgctct gaagttctat cttgtaagat gccacactac gcgggcctgg aatgcgttta
     8581 ctcaatgcac gaaccgtggt gacaagtatc gctttttcgt tattaagcaa ttgctcgata
     8641 ctctcctgcc gagccgctac gaaagccgga ttggcttccc ggtcatcata gggtaaaagt
     8701 tcgtcttccg ggaattgtaa aatcgggatt ttgtgggttg tatagtcttg cagcgttgtc
     8761 agatcatcga gtacacgacc atacgtcaaa ctgtccggca caacgataac ggataagcgg
     8821 tcattcacat aggccgactg agccgctaaa atcccaagca ccgcatccat cgaaccgaga
     8881 tacccgtcca ccatgatcgt cttcttttcg gcgatatggc gaagtaaatt ttgcgcagat
     8941 tcagaggacg ttatacgctg aagaactata tcaagcatgt atagagactt cgattagata
     9001 tattcgccga aaagcgtgtg gccgtcggca cgtgcaatac gaatacgagc atactcaccg
     9061 atggttttat cggacttagg tatgatgata atctgtcctt tgtcggtacg cgcgcaataa
     9121 taattatccg atttgcggct aacttcttcg attaatactt cgcgttcttt accgatctca
     9181 cggtgtaaag attttaatcc gttttccttc tggacttcaa taatctgttg caagcgtcgt
     9241 gacttctccg cttctgttac atcatcgggc attgtatacg ctttagtccc aatacgcgcc
     9301 gaatatttaa acataaatgc cgagtcaaat tgtaccgtac gaaacagttc caatgtatcc
     9361 aaatgatcac gttcggtttc cgatggaaac ccggtgataa tatcggttga aatggacaca
     9421 tccggaatta tgcggcgcac tgtatcaata agtttaagat attcttcgcg tgtatatgta
     9481 cggttcataa gttccaaaat acgattggaa cccgattgca caggaatatg aagctgtttg
     9541 caaatattat cgcgttgtgc gatgatgtgc agaagatgtt cggggaaatc ctttggatgc
     9601 ggagaagtaa accgaatcct acgcaccccg ggcacttcac tgacgcgatt catcaactca
     9661 gcgaaatcct tttgttgcgg atcacggtat gaattgacat tttgacccaa taacccgatt
     9721 tctgtatagc cttgcgctac agctttgtgt acttcttcga ctatactttc aacggaacga
     9781 ctgcgctccc ggccccgcgt ataaggaacc acacaaaatg tgcaaaaatt atcacatccc
     9841 cgcatgatcg ctatccacgc cgtgatttta ccaagtcgca atggttcaac ctgatcatac
     9901 acctccgtgc gtgacaaacg cgtatccatt tgcagaggtt ttaattgaaa atcacgatat
     9961 tcggataaac tgaagtcctc gccatgttgg gacggcaatc gaatctgcac aagattttgc
    10021 gaagcctgtt ttttttctat caactccggg attttacgat acgaatccgg acctaaaacg
    10081 acattgacat acgggcgttt ggcgtggatt tcttcttcaa gatgttttgc catacaaccc
    10141 aaaacaccaa tgatgagatg aggtttctct tctttgagtt tcttaaatcg cgttaactgg
    10201 ccccacacac gctgctcggc attctcacga atagcacatg tattcataaa aatcgcatcg
    10261 gccgtttcat aatcatccac cagatcataa tccatatcgc gcataatgct ggcgaccaac
    10321 tccgaatcat acacattcat ctgacaaccg taggtttcca aatataaacg tttacggttg
    10381 ccttgcaggc gcgaacgtac atcggcaatg gattcaggtg tatgaatctc ttcaaaggct
    10441 gtatcggaca tgggattact cattgatcag agagtccgta aggctgagtg caattccaga
    10501 gagcatgact tccggcccca aagtcgtaag atcaccggac agcatttccg tatccacatt
    10561 ggcatataat gagcaagctg cccctttttc tatcgtaaga ccggaaagtt ttccattctg
    10621 tacgccgaca aaagttactt cattcaatag ttcgctgacg acataaccgc tgcaaaaatg
    10681 aagttgcaga tgcgcgttgg gtgtatatac agtgataagc gtccccttac gacggccaat
    10741 cgggatctcc ggatatattt ccaatgtaag ttttcgtccc tcggtctgat tttcaagata
    10801 cattctgtaa tgatcgccaa aatcctgttt ctccactccc aaacttttgg caatgcgttc
    10861 cacatctacc ggcgacaagt taaatttatg ttcatcttct gatttggttt tttttgtttt
    10921 gagcgcaacg gaacgtgttt tatcggtttt tttaattacc gatttaacgc gtttggaaac
    10981 tgcgggttga gttttctttt tctttttcat gattgtgtat tttgtgtatc tacaattact
    11041 gcgcctaaat cgttatctac ggaacggttt tttcgcagaa gttcagattc ttttaattct
    11101 tgttcgattt gtggaaacgt ataacgtaca tctccgttat tattcaatgt tgtttcaccg
    11161 ttaaaatctc tcaacatgct atccaaagcc tcctggacat cgtgtttttt gagaacttct
    11221 tcccctttgg atttaccatt gatatgagtt acaacttcat ccacggttgc agaggccgac
    11281 ggcatcgaaa acaaataccg cataattcgt ttccgtacat tcatcgcccg acggtttcgt
    11341 ttttgtttaa ttatagcaaa cgaacgaaac aacggtataa taaagaatat cagcgaaaaa
    11401 acaaagggaa cccatcctaa aaacacgccg atccatgggg attcattcat ccatggcata
    11461 tcctcgagag gccggtaata cgttttgaga ataagcacgg aaaaagccaa attaaaaata
    11521 ttcatgaaac tgataagcgc attgcgtccg gtcgtattgc ccgtcagttc ataatcaggt
    11581 tcatattcat cccagtaata ttcaattgca ccatcctgcg cactttcttt ctttcgtgtg
    11641 atctgatcaa agcgtccgta gataactccg ttgtcggtaa acaaaatttc gccttggaat
    11701 cgcacaatac aatcggataa aaacgattcc gctttctccc tcgtccaacc cgctaacgca
    11761 ataagttcgg ccggtgttat gatccctttt tcacgccgta gatacgccgc cacttcacgg
    11821 gcgttggcta acggatctat ttgaacgcgt tccggaccga atacaaaatc atacacggag
    11881 gccatgaagc tttttttgtt ttcgttgagc ggtgacgggc gcggtgtata gtttttgtaa
    11941 cggtatccgc gattatccac tttgtatccg atatcaccgg tgatcgtacg ccaacggaaa
    12001 atggaaaaaa aaacatcaaa gatcttacct aaattgaccg gcgatcgttt actgtctttg
    12061 ctggccgcaa cggcggcaac gaccatggcg atcaacagga ctaaaaagat accgaaataa
    12121 actaccaacg tgatcgcgat ccaagcctta tagaaccagg taaaaatttt ccaaaaaatt
    12181 tggccgatgg ccgcaacata gtctttccat gtcttagcgc cgcggcgacg caaggagaca
    12241 ccgaatccat atatcagatc gccgttttcc gtgagccgca ggcgacaatc gtattttacc
    12301 atcatggcat ctaatgccga cttggcttcg tctacggaca atccggaaac ggcagaggca
    12361 tccgcgagtg taaaatcacc tttgaataca ctaaccttgc tttccagcag tttgatggct
    12421 ttatcaatat cggtttgtaa cataatgcgt tcgtttgaat ggcaagaatg tatggcactt
    12481 gatgcataaa ttcaacccat aaaaatacct ttcgccttgc gccgtatagc ctcttttgat
    12541 atattgcgcc gccatgcaaa atactatact tcgcgacacg cttaaggtca acgaaatttt
    12601 ctacagcatc cagggcgaat ctacgcacgc cggtaagccg tgtatatttg tgcgtttgac
    12661 gtactgtaac ctgcgctgca catattgtga tacagaatac gctttttata gcgggcggga
    12721 tatgaccgtt gaagatgtca ttcgaacagt tcaatcatat acctgtaaaa ccgttgaggt
    12781 gaccggtggc gaacctcttt tgcaagaacg tgtttacgat ttgatgatac ggctctgcga
    12841 tatgggattt gaggtaatga tcgagaccgg tggcagtctc gatgttgaaa aaatcgatcc
    12901 ccgtgtactg aaaatcgttg atatgaagtg cccctcttcc ggtatgcagg ataaaaatta
    12961 ttaccctaac ctgaacaaac tattaccgac ggatgagtta aaatttgtta tcggcgatcg
    13021 taatgattat gaatgggcga aggctttgat tgctcaatat ccgcatacca aacaaattaa
    13081 ttctatactt ttttcgccgg tatttgacac tatcgaaccg gtttctatgg tcaactggat
    13141 gctggaagat aaacttcaaa cgatagtgcc taatattcgt tttcagatac agttacacaa
    13201 ggtgatatgg tcaccggaaa cacgtggtgt ataatgacaa aaaccgtcaa aattgcaaaa
    13261 gaatttcact gggaaatggc ccatcgcctg ccttatcata cgggcggttg tcagaatatt
    13321 cacggccact cttatattct atgggttgaa atcgaaggac aacctcagga aaacggtatg
    13381 ttacttgact atgcagaaat taaacgaatt gtgaaaccga tgattgacat gctcgaccat
    13441 ggttttatat gcagtcgtga cgataaggca gttcttgcgt ttctgaacaa ctcaaaattc
    13501 aaaatgacta tcgttgattt ttatacgacc gctgagaatt tgtgttatta ttttgcggac
    13561 gccatcgaaa aagaaataat aaaatatcct catgttcatt ccatgaaaat tagaatttgc
    13621 gagacgcgtt cgtcgtatgc cgaaataacc cgatccctga aacgatgaca acggataccc
    13681 attctacatt ggccgtcgtc ctcgcaagcg gcggacttga cagctgtgtc acgacggcta
    13741 ttgcggcaca aactcacgcg cttgcaatgt tgcatgtcaa ctatggtcag cgtacagaaa
    13801 aacgcgaact gcgctcgttt aacgatattt gcaattatta cagtgttcta ccctcacgac
    13861 gcttgatcgt taatatcgaa catctaaaac aaattggcgg ttccagtctg accgataatt
    13921 cgattcctgt ctcatcggct gatcttcata ataaaagcat ccccagttct tatgtgcctt
    13981 ttcgcaatgc caatattttg gccatggccg taagttgggc cgagacgatc aaagccgaaa
    14041 aaatttatat aggcgccgta caggaagatt ccagcggtta ccctgattgc cgatcggatt
    14101 tttttgaagc atacaatcaa atgattcggt tgggcaccaa accgggttcg ggaattgaaa
    14161 tcatcacccc aatcattcat ttacaaaagt cggaaattgt ccgcgaggga ctgcatctga
    14221 atgccccgtt atcgcttaca tggtcgtgct accaatcgga agatgaggcg tgtggagttt
    14281 gtgacagttg tgcattacga ttacggggat ttcaatctgc gggcattgaa gatccgatct
    14341 tatatcgggt aagaccaaaa tatgattagt tgatctgttt gcattcgatt ttcgtagtat
    14401 cagtctctta ttaatttaat tgagggagtt tgttatgggc ttaggtaaaa gggttctcta
    14461 tgcgttagct gtttttattt ttcaagctta cattactgga tgtgcggccg gttttcagga
    14521 tatccgccaa acacaaaagg attatggcat cgaaaaacta ccgtccgctg aggaataccc
    14581 tcaacacgaa gccgttatcg tgcttgaatc tcataaaatt gatgtaagta ttgataataa
    14641 ctttgatgtc ttcactattg aagatgttca cgttgtcaaa cgtatatttc gcaacgtcga
    14701 gaatcacgct gaagtagaaa ttactttggg ctatggagaa aaattaaaat ctatccaagc
    14761 aagggccatt acttctacag ggcaaatttt agagctggag aaaaaagaat ttctgcattc
    14821 caaaggctcg tccgacggtt cagtatttta tagcgatgat cgcacgacac gatttacgtt
    14881 ccgcggtgtt gacaagggct ccatcgtcga atataaatac cgcatacaaa aggattaccc
    14941 ttttcaaatg gatgtatggt atgttcaagg aagtttgccg attttacgca acgaatataa
    15001 gctcttcgtg cccaccatct tgttagataa taactggaat tggtttttta aatcttataa
    15061 ctacgatatt ggtttaccga agattgaaaa agccggcatt tctgcaaata aaactttaga
    15121 cagcaaaaat acctacacat ggttagttca aaacgtacct gcgtttgaac gggaaccgcg
    15181 tatgaactcc tactatcatc atgccggcca cgtacgattt gctccaagtt actggaacag
    15241 ttggaatgct atagccgact ggtattacaa aaaactattt ctacctcaat tgattgtaac
    15301 acctcaaatc tctgaaaaag cccaatcttt gatagcaaac gtcgcggatg aaaagtcaaa
    15361 ggttcaacgc ctgtccgatt atgtaaaaaa aataagatac accgccattg aattaggcat
    15421 tggtggcatt cgtcctaata cgccacaaac cgtctatgat cgtcaatacg gtgattgtaa
    15481 ggataaagcc acgttattga tcgcaatgct caaatctgtc gggattacgg cccatccggt
    15541 acttgtaaga acagccaatt ccggttttgt ggatgctcag tttccgagtt ggaattttaa
    15601 ccacatgatc gtcaaaatag cgttgtccgg aggaaaaaca atttggacag atcctacatc
    15661 cgaaaccagt cagttcggat ttcttccgtg gtctgatgaa gatattgatg tatttgtgct
    15721 gtaccccgac ggtaccggta tgattgaaca tacacccact tcgtcacata caaataattt
    15781 aaccgatgta catattcagg gtgaagctat cggcgatatg ggaatgaaat ttgaaattaa
    15841 aattgatttt tttggcaatg attacagcta ttatttgtat cgtcttaggg atatgtccca
    15901 aaatgaactt gaaaattttt gtaaaaactt aattgttgat gaatatttaa attccaaaat
    15961 aagtaatgtt cgttttagtg aaaacgacac ggccggaaaa ccgctctctc ttgtttttga
    16021 aacgaccgtt gaaaaagtca ttcaagcgca aggtgattta tattttgcca atattgaccc
    16081 gtttaagttg tttgaaagtt ggagctggct tgcttcggaa caacgtcgtt tccccattcg
    16141 tacaatttat ccttatctaa ttaagaaacg gatagaaatt aaatttcccg ccaatcttac
    16201 tatacgaaac ttgccaaaac ctcataatga gtccatagac tattttagct ataaccgatc
    16261 ttatcaacaa gccggtaatg atgcgttaat ttttaatgaa acatacgccg tcacggaacg
    16321 atccgttcct gccatagaat ataataccgt tcgaaagttc tttgataaaa ttaaatcagg
    16381 tatggctgag aaggtaatac ttacaaaata agagcatttg ataataaaaa catttgatat
    16441 cctaaaacaa aaaagccgac ttaaaatcgg ctttttttgt tggcggagag ggtgagattc
    16501 gaactcacgg tacccttttg ggggtacaca cgctttccag gcgtgacagt taaaccactc
    16561 ctgcacctct ccgtggctct tggcctcata ttaaacaaac gcttaatatg aaaatatttt
    16621 tatgcgcgcg aaaaatagac aagcacgctc aataatgcaa ggaatttatt gattttgctt
    16681 tttttcagcg agtttttgct tcaaaatttc attcgtcatg gccggattgg tccttccttg
    16741 cgacagcttc atcacttgac cgacaaaatg accaaacacg gcttcttttc cgccaagata
    16801 ttcctgcaat tgtttttgat tcaattcaaa catacgatca ataatttttt caatctctcc
    16861 gcggtccgtg atttggagca aaccttttgt tttcactata gcttcggcat ccgcaccgtc
    16921 ctttgccatt tcttcaaata ctatcttcgc aatttttccg ctgatcgtat tatcggatac
    16981 catacgaagt aatttcccaa gattggccgg tgaaatgata aaggcatcgg catcctgttt
    17041 gcgctccttc aacaaagcca tgacctcgcc cataataaaa ttactggcca acttgccgtc
    17101 gcttacgtgt tttagcgttt cttcaaaata attggctaat gatttactga gcgtcaaaac
    17161 gtcggcatca tacttaggaa tgccgtattg ttgaacaaac cgatcccgac gcgctccggg
    17221 taactcggac aaactaccgg cgatttcatc cacccaggct ttagtaacac gtaaaggtac
    17281 cagatccggc tcgggaaaat agcgatagtc atgcgcttct tctttggacc gcaatgcgcg
    17341 tgaaacacct tgattgtcgt cccacaacaa cgtctgttga ctgacactac cgccgttttg
    17401 cagaatatct gtttgacgag ccatttcgta attcaaagcg cgttctaccg ctttgaatga
    17461 attcatgttt ttaatttccg ttttggtgcc aaatttctcc tgacctacgg gacgcatgga
    17521 tatattgaca tcacaccgca agctgccttc ttccatattt ccatcacaaa tttccaaata
    17581 ctgtacagtc tggcgaattt tggtcaaata tgcgacaacc tctgccggag tccgaaagtc
    17641 cgcttccgtt acaatctcta tcaagggggt accgcagcgg ttaagatcaa caaaggtttt
    17701 ttcgccgccg gtgagggcat cgtcgtggat agatttgccc gcatcttctt ccatatgtat
    17761 gcgttttata cgaatccgct ttacggcttc gccgacttca atatccaccc acccgttttc
    17821 acataaaggt ttgtcaaatt gggatatttg ataaccttta gtcagatccg gataaaaata
    17881 attttttcga gcaaaaatac tttcttcggc gataacacaa tgcgtagcca atcccatacg
    17941 aatggcaaaa tccactgctc ttttattgag caccggtaac gcgccgggca atgccagaca
    18001 gaccggacaa gtttgcgtat tgggcatcgc accgtatttg gtgctgcagg tacaaaacgc
    18061 tttggattgg gtaagaagct gagcatgaac ttccagaccc attaccggct catattgtat
    18121 aacggacata ctattcctct tacatcataa atgttttttc gcgttcctga ccgatagaaa
    18181 cgatcttgat acgtgcatgg ccaagataat cttcgataaa tttgagatac gcttgtgctt
    18241 tttgcggcaa ctcaacataa ttttttacgc cggatgtgtc ggacttccat ccagaaacgg
    18301 tttcatacac aggctctaca cgttccaact caccggcatc cgtcacataa tgatcaatgc
    18361 gtttaccatc caccttatag ccgacacata ttttcagctc ctctaaagtg tccagcacat
    18421 ccaacttggt aatggctaat tcatcaatgc cgttaaccat catagcatat ttgagttgca
    18481 ccagatccaa ccatccgcaa cgacgcggtc tgccggtcgt agcgccaaat tctccgccga
    18541 gttcacgcac cttttgacca aaaacttcat caaattctgt tggaaaaggc ccgttaccga
    18601 cacgcgtatt gtaagccttg actacaccca taacgtggtt gatataggaa ggacctatac
    18661 ccaatcccgt acaagcgcct ccggcgctgg cattggatga agtcacaaaa ggatatgtcc
    18721 cgtgatcaat atccagcaag gtaccttggg cgccttcgag taaaatctct tttccggttt
    18781 gattggactc aaaaagatat ttgacactgt caccgatcag cggcttgagc tgctggccga
    18841 tttctttaaa ttgtttgatc gtcggctcga tcggtaattc ttcggagcca aatgctttta
    18901 ttattttatt tttttccgta atagccttgg aaagttttcg ctccaacgtg tccggatgaa
    18961 tcaaatcaat cgcacgaata ccgctgcgat caattttatc cacatacgcc ggtccaatac
    19021 ctcgcttagt cgtaccgacc tgattttcac caaggtactt ctctttagct tggtcaatga
    19081 gtttatggta cggcaaaata atatgcgcat gcggactgat catcaaacga tcgtgcatcg
    19141 cgatccccat ttcagacagc atttttatct cgtcaaaaag cgcaatagga tctatcacta
    19201 caccgttgcc gagaatacac ataacgccct catgcaatat acctgagggt atcagatgca
    19261 aaacgtattt tttatttcca tgaacgatgg tatgccctgc attagcgccg ccttgataac
    19321 gcgctacgac atgtgcctga ctggctaata aatcaacaat tttcccttta ccttcatctc
    19381 cccattgggc gccgataaca attcgaacag acatagtttt ttccgatcag acaaaaaata
    19441 acccaaaatc tgtttcagat cttgggttat tatgtttaaa aataatttaa tttttagaca
    19501 aaacttttaa ccccgtcatc cacagaatcg tattgcttaa atacggtcgc caattttgtt
    19561 atttgaaata tctgtttgat tttcgaagac agattggtca agcgtaaatc accgccgtta
    19621 ttttttaccg tagtcagatt gctgatcaga atacccagtc ctgagctatt gataaggtca
    19681 acggattgca tattaacgac aaaattcttt tttccttgct ttatgcgggt atgaatttca
    19741 tcattaagct gtgttgcatc cggaccgccc atgatgttgc cttttaattc caatacgaca
    19801 acaccgttat tttctgcaat attataatcc atagcggtaa cctctaaatt gactggcgta
    19861 acataaacca aaaaagcatc acgatcaagc gcagattatt acttgacttt aatatcttct
    19921 ccggcattcc attcgatcag gatcgggctc gccacgtaaa tggatgaata cgttccaacg
    19981 acaataccgg tcaacagagc ccatgcaaat gtgcgaataa cctcaccgcc gatcatcagc
    20041 aatacgataa ccgacaacaa ggttaatgcc gaggtcatca ccgtacggct cagagtttca
    20101 ttaatacttt tgttgatgac ttctccaaag gtcgtaccgc gtttgataag gcgcgtattc
    20161 tcacgaatac gatcaaatac gactatggta tcgtttacgg agtaaccaat gatggtcagc
    20221 aaagcggcaa cggcacccag cgaaaactca agttgcaacg cacaaaatag accgatggtg
    20281 atgatcacat catggaacaa agccgccacg gcagccatgc cccatttcca atgaaaacga
    20341 atcgcaaggt atacaacaat gaacagaagt ccccaaatcg aagcccagat ggcggaaact
    20401 ttaagttcat caccgatctt ggggccaacc tgactcaccg aacgtaattc aaccgaattg
    20461 ccgggaaagt tatcgttcat cagctttaaa acggtcggag ctacatcgtt attttgttcc
    20521 aaaacgtgta ttaacacttc atttccatta ccgaagtctt gaatacggtc agagctatac
    20581 tttgatgaaa cgatgcttcg aactttttcc aactccactt tggagctaaa cttaacttcg
    20641 atggaggtac caccgacgaa gtcaacaccg taattaagtc ctcggaaaaa gaataacaac
    20701 aagctgatta aaatgagtaa acccgaaaaa atgtacgcat tgcgacgcgt atcgacaagc
    20761 gggtaatttt tatttacaaa caattccatt atctatgctc ctcgaaaatt tcgttatttt
    20821 atgcgctggt tttggtatag gaaataccga tactcatctg acgctcaatc gtagaacgat
    20881 cataaatgag atccatgata aagcgcgaca caaacacacc ggtgaagatc gtggtgacga
    20941 taccgatcat cagcgtcaaa gcaaaacctt tgatcggacc ggtaccaaat tggaacaaca
    21001 ctatacctgt accaaatgtt gtaagattgg catctacgat cgtaacaaaa gcttttgcaa
    21061 aaccggtttc aatcgccgca cgcactgttt ttccgaggcg aatttcttca cgaatacgtt
    21121 cataaattag cacgttggca tcaaccgcca tacccatcgt aagcacgata cctgcaatac
    21181 caggcagggt aagcgtaaaa ccgaaaccgg tgagaactcc gaaaagtaat agaacgttga
    21241 ttacaagcgc catatccgcc acagcgccgg acatacggta ataagccacc atcgctaaag
    21301 cgataaccac gaagcctatc cagaatgcca tttttccgct gtcaatcgaa tccgctccca
    21361 acgaagggcc gatcgtacgt tcttccatca cgtgaaccgg cgcgggaagt gaacctgcac
    21421 gcagtgcgat agcaaggctc ttggcttcga taaggttttc catgcctgtg atttgtgtat
    21481 taccgcctga aattttttcc tgaatattcg gggcggattg aactttgtta tcaagaacaa
    21541 cggcgatgcg tttgccgaca ttagcaccgg tcacgatcgc ccaccgtttg ctgccttcgg
    21601 gtgtcatttt aagcgtaacg atcgggcgtc cggccaatcc cggatccata ccctgataaa
    21661 tatcctgttt cgcttcggtg atcacggcgc ccgataattc gggttgtttt ttcaccaaat
    21721 acaacgcgtg gtggccttca aaaataggct tagaactgaa gatgatatca ttggactcac
    21781 cgatcacctg ttgtacaccc ggcttcattt tgacacgctc gccgacctga taatagagca
    21841 tggatttaac gtattcaaaa ttattttcgg gaaccaatac atcatattga gacgaaacca
    21901 aaagtttcgt taatggttta tccagattag ctaacgcgga agaatctttt tgtgaggaat
    21961 cggaagaaga cttttgaagt tccgccgaca accctgtacc gccatccgat gattttttcg
    22021 acgtatcggc cgaaacggca ccggcgctga ttttgccgct aaccgtatct acttttgcaa
    22081 aatacacatc caagcgttcc aacgctgtac ccaaatcctg cgggtctttg actagcttaa
    22141 attcaagcaa cgccgtttgc tggatcagat ttttagcttg agccggatct tcgacgcccg
    22201 gtaattccac gattatacga tggtcgcctt gtttttgaat agtgggttcg ctcacgccaa
    22261 attgatcaac gcgattacga atgatctgct gcgctctgtc cacggcatct ttggattgtt
    22321 catccataaa cttaaccacg tccgcattgt ccttgaagcg ttcaccccaa taggccggca
    22381 tagataattt tgcatcttca aaatgagtac gtaaaacatt ggaaaaaaca gcatccgggt
    22441 ttttacgata ttcttcagcc gctttttcaa aaacccgtgt gaactcggca tctttggttt
    22501 tggctatatt ttcatagagc gtcaccacat ccgtttccat cacaagatgt ataccgcctt
    22561 tgaggtcaag gcctaaattg atagatttag attgcagttc attaagctct ttcgtgcctt
    22621 cgggtgtttt gagcatttct tttttctgtg catcagacaa attataaaat ttgaatgaag
    22681 gccaaagcgc gaacagcgat aaggctgcac agatcacaat gatcaccgct ctgacatttt
    22741 tactcttaat cattcgttca tatcctccga taaatctatt aagtatttaa ttatcagcag
    22801 ttttgaaaaa aacgcgccta atatagatgg ccgtattcat aatgtcaagt ttattaaaaa
    22861 tgaaggaaaa tgcctgatct aatccgtaca accttgcgcc gctaatgacg aatcatcaat
    22921 cgccgtgcgt atgaatttat acgatgtcac atcgtcagtt aacgtcaatg tatcctttac
    22981 aacttcccag tttatcgtaa tgtagccatc gataataaag tcatcaccat caacggtata
    23041 ttgataaaat tgaatatcaa agcaagtatc gccatccgct ttgacggcat caacatacac
    23101 tactttatca aagtctaaat aattaacata accgtcaaac tctccatacc actccgtacc
    23161 attaagtgga aaattaggtt tgtcgtcgca accggagaaa gtcaatatcg tgcatgtact
    23221 aacaaccaaa aaagataaaa atctattaat cttcatatcg tccccttttt ttgacaaagc
    23281 gctttatttt atttttatag aatctgcgtt tcctgcatta tactgaatta aatattccaa
    23341 cggccaccaa gtgacaatgc cggtgccgcc gcccgtaaca acggctttac cgtccggaga
    23401 agccgacata gaccatattc ctcgctcttg tgctttacta atatctttta gtctttccaa
    23461 accattggtt ttccacaatg tcagatcacc atccccgtca ccggtcacaa aatagtttcc
    23521 accctcaagc caacacattt ttttaatttc accatcataa acgaccgttt gttcgtgcgc
    23581 gtcgtttgtt cgattccaaa ctgtaatggc gtctacactg cctgtgagta tccgattccc
    23641 ttcaggaaat agtaacatcg cattgatagg atcaccttca gcgtaggtac gaacatcatg
    23701 ccccgctttc atatcccaaa ttaaaatttt tttatcccag cttaccgaca caaatacatt
    23761 ggggtctgac gtaaagatta tatctgtaac cgcatcataa tgtccgtacg gggacggaac
    23821 ctcgatggga tggccagaaa aaaatttagt ttgttttcct gtttccacat ccaaaaaaag
    23881 gatattaaaa aaattaccga caatcaattc tctaccatcc ggcgaatatc tggcaagctg
    23941 aatatcattc gtttgtttgt caataaacca ctggcgatgg cggtcagcga aagaatacat
    24001 cccgatactt ccggtggatt gcgtattgtc acttgttgta ataaaaaatc gattcccatc
    24061 cggcgaaaac ccgtttacta aaacggcatc cggcattatg tccgataccc atattacatt
    24121 tccggttgca aattctataa ttttcgtgtg cttgttttct ccgccacaga tcagaaactt
    24181 gccatccggt gaaaattgta cggaattgac gattttaccg tcaccgatag aaaatgtttt
    24241 gggttgttct gtcggttgag gattatttcc agcgccacgg caggcaatca acactgaaaa
    24301 gagaatggga ataaactggc ttcgcatata ttttttccac aatggtttac gattttgtcg
    24361 tggtaattgt ctaaaaaggg cactaaatta gcccgttcaa acttgagaat caagcgtaga
    24421 atcttaatta ctcacgttct attttttgaa taccacgcta tcatttttaa aggttaaata
    24481 tggccgttaa acgaccgaca aaggcaaaaa aaacaaaaca tgtttcccct aaaaacaact
    24541 cgcgctctaa aaaacaacca ctgattaaaa ctatagctaa tcgtaaagtc aaaaagactt
    24601 ttcccgtcag catcaatgtg ccccagatcg aaaacctttt gtccgaactt gaatacggcg
    24661 aagaactggc tgaatattac gatctgattt attcagaaaa ggtaatacac caaattcaat
    24721 ctcctttttt tcaaatgtta atcaaaaaat acggcattcg gtcggcttgc gatttatctt
    24781 gccgttccgg tcaaacgctt gctgcgctat ccaaactggg catcaaaaat ctaaccggtg
    24841 ttgacgtaag ctccaaaatg cttgatcttg ctcggaagcg ttgcccctct aaaacacaat
    24901 tttatcaaag tgagttattt ctcgctccgc acaccatcgg cgatgaaacc aagtttgatc
    24961 ttgttttatg ttcgcgcgat gcattgcctt tggttctgga tgacgaaggc cttctagggt
    25021 ttatggcgca ggcgcgtaat ctgctttcac ccaacggtat tcttgtcata gaaacatgga
    25081 attacgataa aatctggcgc cgaaaagaac gttttatgcc tgtgatggat cgctgcagta
    25141 ataaacaatc tctgctgttt tttatcgaaa acgattttca cggcgagttg ttggtgcata
    25201 atgttgtcaa actcgaaaaa aacaaatttg aatggttctt acgaaccatt tcaacaccca
    25261 ttcgcccgct cacgcgcaac gaaatggagt tcttcgtaaa agaagcaggg tttgaaaaat
    25321 acggtttttt gggtaactac gcaggctcgc catttaacac gacagagagt gcgtattgta
    25381 tcatgcttgc gatgaataat acaaacacca taacggtatc ataaccgttt ttttgaattt
    25441 gttaataact cttgcgcggg actatatatt aagttatact gccccactac gatctatgat
    25501 catgagtttc ttttccgcct tcaattccgc taccggattt gtgatcgctt caaaagtggc
    25561 tcaaagttca ggtctcggcc tcttacttcc gtatcataat cgaatttaat tttttaacga
    25621 ggtattcgtg cgtccgagtt tttcacgccg tatatttcgt tggcattttt tatcgcttta
    25681 ggatctcatc cgtaaagcac tttttaagaa agaatgatgt atggctttca agcaacttca
    25741 tgtcatcgaa cctattttac aagcactcaa atcggagggt tataccaaac caacccctat
    25801 tcaggaacaa gctatacctc tgattttgaa tcacaaagat ttgctcggct gcgcacaaac
    25861 gggtacggga aaaacagccg cattcgcgat tccgatattg caactcttat ttcaatcgtc
    25921 atcggttcat atcccgcgta aaacccacgc gcttatttta actccgacgc gtgaactggc
    25981 aattcaaatc ggtgaaagtt ttacggcgta tggtcgtcat acgggattgc gacatgccgt
    26041 gatttttggc ggtgtatcgc aacatgctca aactcaggca ttgaaagtcg gcgtcgatat
    26101 ccttatcgcc accccgggcc gattactgga tctgatgcag caggggttcg tccaccttca
    26161 acaacttaag atttttgtat tagatgaagc agatcgcatg ttggacatgg gctttataca
    26221 tgatgtaaaa aagattgtcg gtaagttacc ttccaaacgc cagacgctcc ttttttcggc
    26281 cacgatgcct gctgaaattc aaaagttggc acattcaatt ttatcacatc ccgaacaagt
    26341 ctccgtcacg ccggtgtcat ccacagctga aaaaatcgaa caagcggttt attttgtcaa
    26401 taaaccggat aaaaaaaatt tacttattca tgttttgcga aatcctgaga ttacttcagc
    26461 tctgattttt acacgaacca aacacggagc cgataaagta acgcgggatt tgctcaaagc
    26521 cggtgttaca gcggaagcta ttcatggcaa taaatcccaa aacgcccgtc aacgtgcttt
    26581 gggaaatttt aaaaatgcta aaacccgcgt tctggtagct acagatatcg ctgcgcgcgg
    26641 gattgatgtg gataatttat cccatgtgat aaattacgaa atccctaata taccagaaac
    26701 ctatgttcac cgaatcggtc gcacgggacg agccggtatg agcggtacag ccatttcttt
    26761 ttgtgatccg gaggaacgcg cctatttgaa agatatacag aaattaatcg gaaaaactat
    26821 cccggttatg gatatgccga tcgctgtgac acccgcaaaa gctgcggcta acaccttaca
    26881 tgatcatggt ataaaaccgc aacagactaa acaacacggg cgcccacaac acaaaacggg
    26941 ttcatcggat cgaagaacta aacaaaaaac ccaacaccaa accggtaagc ccaaagatca
    27001 tcgcagttgg tttaagaata cttttaaaaa gagagaagac tagataaaat aaaagctccc
    27061 ttcgaaatga agggagcttt tgtgtagcgc gtacgggaat cgaacccgta ttacagcctt
    27121 gagagggcta cgtcctaacc gttagacgaa cgcgccatga actgcggaat atatacaccc
    27181 gatcccgcgt ttgtcaagca ggtttatcat ttatacgtaa ttatttttaa tttaaaatag
    27241 attaaaatta tgacagagat tcaatcgccg gaaatgttag atgctttgct ggaaaaatcg
    27301 ttaaatacta cggtgcttct cttcaaacac agcaacagct gcgggactag cgcgtacgct
    27361 ttggaacaag tagaaacttt tttgtcggaa tatccggaat ggaatagtcg cagcggcatg
    27421 attgtcgttc agacgcaccg cccgatttct aaccgcgttg ccgatttatt tgctatcacc
    27481 catcaaagcc cgcagatttt tgtcattcaa aacaaagaag tcctatggca taaatcgcat
    27541 cacagcatca ccagcgacgc gattaaaaaa atcgtcctct cgatagtcta acacacgtgg
    27601 aaataagaga taccgtatcc gttcagaggt taacttcata tcccaaaact atggttttgt
    27661 aacggaaagg ttctactttt tgggtaaatt ccgtttgcga ataagcggct cgtatgaata
    27721 atcgttgatt ggcggctgtt tcatatgaaa gtttgatgtg cgcacgatta tcctgaacgg
    27781 aattggtata taacaaattt ctagtgattt tattctctcg cggtatatca cgcctaacgt
    27841 aatcggctaa aaaatataac ccccaacgtt cattgatatg tttacctata acaatatgcg
    27901 cttcctgctc taaagaataa ttaagggtat atttggatac acgccatgat gcgatatagc
    27961 gtccattgat aatccatgta cccgagtagt ccaaccctgt ttccataccg ttgcgccagc
    28021 cgttattttt actcggataa gtcaatggat ccgtcgtgga agcaaaaaaa cgttccgtaa
    28081 gaaaaccggt gaaaactttc aaatattctg cgctggacca tactaatcca ataacgccgg
    28141 cgcgggacgc aaagcgattt tcatgatcca ctttggagat attacgttca aatcgcaatt
    28201 ctgcaaacgc acccatcctt tgtccgaaat aatattcgcc tcgggattga agattataca
    28261 aagtcgcgcg tacatccggt cctgaaagcc catactgttg agtgcgccaa ttagtattta
    28321 acgaccacac ccatgcgccg ctcgcataaa cgtattcggt gctggcatgc gaagtccatg
    28381 catggcgttc gaatgacgta ttataatctt cgtaacgtcc acgcccttga aacgtaagct
    28441 ggtgttctcc gaacacaaat tgatatcgcc ctgaacctgc tgcagccatt aaaaattgtc
    28501 gactggttgc cgttcgattt gatataacag gaccagtctc gccgccaata cgccaatcaa
    28561 aagtaccggc gtatagcgtc gtttgaaaac atgcaaaata gatgcctatg aagtttattc
    28621 gggccaacag aagtgttttg gctttcatgg gttgagtttg tccgtaagca aatcacggta
    28681 agcaaccaac tgtttatcgg cttctttgag taactgaatg taatccgatt ccgacatgaa
    28741 ctcggacatg ctcaaaactt tttcataatt acggacaata ttcgaaggat ttccgatctg
    28801 acgcattact gaaatcaccg ttttggcttg cacattttgc ggattgatat caattccgtt
    28861 attcacaccg tcgtttttta taaccgaagt accattacga gaaaaaccgg cccaggttcc
    28921 cgcatcatat gcatcttgta taaaatccgt cgtacgtttt ctcagagaca ccagggattc
    28981 gtatactttt cgttgcgtac gaatctgatc tacatattta tccacttcga taatagccgc
    29041 ctgtaggtat tctttgcgaa cctctttttc aaaaggatcc gtaatcttgt acaaatctat
    29101 ttttaataat gttaccggat catacgatag attgttcatc ggaggcgtga tgagtagctt
    29161 tttttctgtc caatatcgca attgtttttc gatagaagaa gtgttttggc gtttttgttg
    29221 gatacggagc gaatcaatga tatagtcgta ttcgctgttg agttgaatac gtaacgtttc
    29281 gattcgttta tgatagtcct ccatccgtcg ttgattggcc ttaatttgtt ctgacaaggt
    29341 caatccttta gcgagtgatc gtgctatacg ttctttatcg ggctgaatgg attttttttg
    29401 ctggacaatt tcatctgcat tttctttcat gcgctgattg aggctgtctg ctgcatgtgc
    29461 cgatgcacgc caagcaaaaa tagtttgccc gtaaaacaac tccttttctt ccatgttttg
    29521 tgcatggaga cggcctatgc caatcacccc aatcgtaata aaaataaacc gattcataag
    29581 agttggaatt tttccatttt ataataaagg ctgctcgtag gaatgcctaa tagttttgca
    29641 gcctgatttt tgatattatt gctctttttc aatgcagcca taattatacc cttttcgaaa
    29701 gcttccaacg cctgctccaa cggcaaattc tcaaaacccg acatgagcga tttagaagga
    29761 cccgtgatat gttgagtaat catggcatca tctattattt cgttttcaga tataacacac
    29821 aaacgttcaa tcagattttg caattcgcga atatttcctg gccaagcatg ctttttcaaa
    29881 gtttccatcg cttcgtccga tatctgtttt gaactttgac cgcgggttcc ggcatattga
    29941 ttgagaaaat gatgcacgag cagcggtata tcttcctgtc gttcacgaag cggcggcaac
    30001 gaaatgggaa tgacccgtaa tctataaaaa agatcttcac gaaatttttc ttcttgcacc
    30061 attttagaca gcgagcgatg agtggcggct ataatgcgta catttacggc aatcgtatct
    30121 tcaccgccga cacgttcaaa ttctctttcc tgcattactc gaagtaaacg cacctgcatg
    30181 gcctgcgaaa tgtcaccgat ctcgtcaaga aacagggttc caccgtcagc taattcgaaa
    30241 cgacctttct ttcttttgat agccccggta aaggcgcctt tttcatgccc aaaaagctca
    30301 ctttcaagaa gattttctga caatgctccg caattcactt tgacaaacgg tttatcgtga
    30361 cgattacttt tttgatgtat agcgcgcgct acaagctctt taccggttcc gctttcgcct
    30421 tcgataagga tcgtactgtc ttccgatgcc actcggtcaa tcaaattgaa tattttttta
    30481 ataccttgcg atgtccctat catcgcgccg tagctgcgca gcagttcgcc ctgtaataat
    30541 tcgttttctt ccttgaggcg tgttaaaata atttcctggc gaattttttc gacaagcctg
    30601 cctacccgaa aacgtaattc ctccggtgaa aacggttttg tgagaaaatc agcggcgcct
    30661 tttcgcatgg catgtacagc cgtctcgacc gtaccaaagg ccgaaacaac cataacggct
    30721 gtttgcgatt ggatcgtttt gacctgttct aagacgtcca tgcccgacat gccatccatt
    30781 ttcaaatccg tgatgaccaa cgataacggc ttttgtttaa aaaattttaa agcactctcc
    30841 ccatcggaaa aggcatggac ttcaaaccct tcgcggcgta aactttccac caatcccatt
    30901 cgcatggaag cgttatcttc aacaatcatt attttcatgg attcctgcat acgtcaaatc
    30961 gtttgtttaa gccgcatcgt cacagagcaa cctgattcag gacggttttc gatatggatc
    31021 gttgccccgt tggcggtaca aagtttttta cagatcgcaa gtcctaatcc gttaccatgt
    31081 ccgcttctcg taaaaaacgg ttcaaaaacc tgagatagcc atttttcatc aatcccctta
    31141 ccgttatcac gaattgatac atgaatataa gagtcgtgta ttttagattc tacttcgatt
    31201 ttcccgaaca tttcaagcgc ttcaactgca ttggcaaaca aattaagcat aatctgacga
    31261 aaatgctgtg gatcaaaaaa aatcatgggt acgcttaagc tgtaattaat ttcaattttt
    31321 ttatttttta tttcgttcaa aagtaattgc tccacttcga cacacaacga cctgatgtct
    31381 ataagttggc ctacagcttg tgccggacgt ccgaattcta aatattggtg aacaagattt
    31441 ttaagtcctg aaacttcctt tcttatttgc cggatgtacg cgctttgttg tgactctggc
    31501 ggcatatcct cgtaaaccaa gccggccatc agttcgatac cgcccaaagg atttcttatt
    31561 tcatgcgcga tttgcgccaa cagttcctct ttttctttgt gacgaccttg tatatccgta
    31621 cgcatttgtt ccatagccgt ggccaacgag gccaattcgc ctttgacatg tttaggcgct
    31681 tcggtatccc attccccacg tcccaacgaa cggctgaacc gcaccatttg gtcaatcggt
    31741 ttagtaatcg ttttagccag ccaaaaactt ccaaataaag taaatactaa tgccgttccg
    31801 cttatgaacc aaaacacaaa ggataagttt tcaatgtaca tcaattgatc ggccttggca
    31861 cgtacaccgg cccaatattc atcatctatt ctacaaaatc cccacatata ccattcgttg
    31921 tcattaccca aaaagggtaa cgtcgtcgaa gtttcgcctg gagctaatcg ttcaatttca
    31981 gattgactga gcagtaatgt aggatccgga tggatataat cggattcatc atcactcgtc
    32041 atcaccattc gcaatgccgc atcaaaaata aatgcactcg ccagttcgtg tcctactttt
    32101 ttcttcttta gatatcgctt gtaaaactgt gtggctacgg agttttccga atagggttgg
    32161 acaaatttta tatttcccat atccatatcc gttgcgatcg ctttggcgga catacgcacc
    32221 tgacggctta attgatccag ataaacttct cggacggcta cgtaactaaa acgcgaaagc
    32281 acgatgatga gaatcatcgt gatcacgctg aaaagaaccc cgatccgagt acgcatcgtc
    32341 catcggaatg tcataagctt ttttacttaa agttgctcct tttgcatatt aattgcaaca
    32401 gccgctcact tgttttaaaa atacacccgc ccgggttatg aggagggaga gggtttataa
    32461 aattcccggg cgagtgctct tcattaccgc acctgcaaaa tttgcaggcc gtctatattt
    32521 acttgactaa aagttatacc accacaaaat acttccaatt taaaattttc accgtgtcgc
    32581 atttcaaatt cttcagactc atagagtttc cactcaccgc tttcgacgga caaatcaatc
    32641 cgacttagat tttcagcatc ggacgccgta agtgaaaata aagcgttgct ggcactgttt
    32701 tcttccatac gtgcccacat tcgtaaacga tagaaacccg aagcgacaac ctgtttttca
    32761 aaaactgctg caggttgaat acatccggcc cccacggcca tactgtattc gcccgcacca
    32821 ggcgccgctt tattgatacg ttgactttcg ttgacatttt tccatgcttt gaggtcattt
    32881 attgattcaa atgacgtttc ataaaagagt cctgaatctt tttcagtgca agatacgact
    32941 gcaataaata gtattgagag gatacctgct cgcgttattt gcatgaaatc actctccttt
    33001 ctaagtaatt gtttgagata actgtacata ctttttaaaa atttagatca cgttattaat
    33061 ggcctgtatc aattaaattt tgaatcgaat cgcaaggcat tgatatttca aaatcttctc
    33121 catccgtcaa aatgtggatg ccttctttgg taaacaatgt tttgcctctg aattcataat
    33181 ctttgaacgt ctgattagga acgtctttaa gctttgactg ataaaatact ctttctattt
    33241 caggctctat ggtaaaagtc ccaggcaccg catcttgcgt catggtacat tcaaagtatc
    33301 cgctgtttgg cacgcagctc tgttcgctca gcacgacgtt ttgaacaata tcgcgatacg
    33361 taaatttaac aaccgtatcc agagaaaatt gatattgaaa gtaacttatc gtggatccgc
    33421 tataatacaa ttcgccaacc ttgcccggat cagaataatt tttgatacct tttatcgtat
    33481 atttggctgt accctccacg tcataggcct ttgcaccgga gccgatgggg tgattatttt
    33541 tataattaaa ctctaaatac atgacatcgc tctttgatgt aggattgcct tcctgatcgg
    33601 gtgattcata tcggtattgc aattcataac tttgatttga tcgctcaaaa cgaacggacc
    33661 aatattgagt ttggtggtta tatctcagag aatcatacat ggatgattct ccttcaaacc
    33721 gaaaacccca aggagatagt atgaacaagc caggtaaatc agcgatctcc ctttgagcga
    33781 ggtaagaagc gttatttaat tgtaccaagc tattgcttag cattgcacgg ccttgagtct
    33841 cgacaatatc gtttaattct tcgcggctca tttttttttc tgatgcgtca taacatgaaa
    33901 tcaaattcaa cgaaatgaac aaaagaccga atattttttt tgctctacac aaaacgaaca
    33961 catcactttc ttattcacca cagattaaaa gttggtcatc atcaaattct acttgtgtcc
    34021 aagctgtaac cacttcacca aaacgaacat acagcgtatc attactcact tcaaaggaac
    34081 cgagacagat atcatccggc atttttatag gtgtgcatat ttttcccttc attttttccg
    34141 tctcagagta cggatagtat gcatattcat aacaacccga aggatgcagt tttatccaat
    34201 aatgatatgt tccattttca aaatgtactc gctcctcttt taaccgcatg acgcttacag
    34261 tttgccattg tgtgccgtct attcctgatt cttttttaga atcacaaccg aataatgaaa
    34321 ctgtaattac acataatagc attatcgctt tatggtgcat aactcgtacc ttcatataag
    34381 ttaatgttct aagcgttcta taattttcaa agttagtatg ggtgcagcag catgaacaca
    34441 tactaattct ttatttatat ggcctgtaaa ttttaccacg ataccgtctt ttttatattc
    34501 atccggtaaa tcatgcacat caaaaatttt gttatcactg gttatcaatt cgaaataacc
    34561 accttcaacg gtcacatact ttatgtagcc gacgcccgat atcttttcgt cggaaccgaa
    34621 caacgcttta tcgttttcgg atgtgccaat attcgcgcct tccgaacacg ccattaagag
    34681 aaataaggtt gttagtatgt ttaacttgat tagcatcgaa gtgaaataga ttagtatgac
    34741 gaaatgttga ataataaagc tacaaactta gtgccaatga ggaacaaaaa tgaagtcagt
    34801 taaaccaata aaaaacaata cagtgagttt tgtttttact cgattttcag ttctcggtta
    34861 tcagaaaacc gacactatag cggatatttg ataagaaatc gggatttgat atgatttgaa
    34921 aagagtcagg ttgggatatt tgatttacaa attacaaaac aatttcatgt tgcgcagata
    34981 tcttagcggc ttcccatccg atgattgccg ttttacgggt tggaccccac atatagccgc
    35041 caaagtttcc tgaagactgg atcactctgt ggcatggtat caaatatgca acgggattac
    35101 tgccaatcgc tgtacccacg gcacgcgaag ctttcggctt acgaatttct ttagcaatgg
    35161 tattatatgt agtcagcttt ccgaagggaa tttttaacaa cgcttcccat actttgagct
    35221 gaaactcgct tccttttaaa tgtaaattga tcgaatgatt ttcaggcgct actacattaa
    35281 aaatattcag cgcattttta tgaaacgaat ccttcttgtg atgatatgtg gcattaggaa
    35341 aatgtgacac taattgcgat aacgctgatt tggggtcatc ggagaacatc atgtaacaca
    35401 ggcctttgga cgtagaagct gaaagtatgt caccaaaagg actttctgaa aaactatagt
    35461 ttatttcgag attttctcct ccatttttat attctccggg cgtcatacct tcgatattaa
    35521 caaaaaggtc atgcaagcgg cttgttcccg ataatccgct ttccatagcg gcatccatca
    35581 aactaatttt acggtctttc aaaatacttt ttgcataatc tatcgttgta aactgtaaaa
    35641 acttttttgg acttaccccg gcccatgccg taaacaaacg ttgaaaatga aacgggctta
    35701 catgtacttt tttagccacg tcatccaaat caggctgttc tttgaaatta accctgatat
    35761 attcgatcgc ttccgctatt cttttgtaat ttatctgctc ttgcgtttcc atatcctctc
    35821 cttttttagg ataaagatac tatctcaaaa ctctcagcaa aacccgattc ttgctatttt
    35881 tggatcaaag taagtcattc tgaaatcatt attcattgtg aatcaaaacc gacgtcatcg
    35941 tcaaagaccc ggcaaccggt ttgtcattaa aaaaacttat tttttcatcc ggtttctgta
    36001 acgccagaat atacagtaga ggcaaaaaat gatctggcgt cggtatggcc atatcaaaag
    36061 cacgtcccat tttatcgtag tgtatgagag cactgtgatc gtttgtaagg attttttgtt
    36121 tcatggtttc attcgcttcg atggcccaat cataaccata ggccgtgtca ttgagtttat
    36181 cccaagcaac catacgcaga ttatgtacca tatttccgct ccccacgatc aaaatgcctt
    36241 tctgccttaa aacgtggaat tctttagcta attcgtagtg gtattcagcg tgttgataat
    36301 ggtcaaggct catttgtatg atcggtatgt cagcattcgg atataaatgt ttgaccacac
    36361 tccagcatcc atgatccaat ccccattgtc catgatccaa gccgactttg gttttggtta
    36421 tgatggattg tgtttctaag gctaaatccg gactaccggg tgcgggatat tctacttcat
    36481 acaacgattt tggaaatcca ccgaagtcgt gaatcgtttt gggtttgtcc atggccgtca
    36541 cataggttcc ccgcgtctcc caatgagctg aaatgcacaa aattgcctta ggccttggta
    36601 agagttctcc tatgcgacgc catcctcgga caaactcatt ttcctctata gcattcatgg
    36661 ggctgccatg accgagaaac aaaatcggca tgctttcagt attaggcaaa gattttgtga
    36721 tggatgaaaa ttcgcgcagt cgcatattaa ctccggctaa tggtattaat gccatcgtat
    36781 gaataaattg ttttcgattc attaacaaaa ctataagaag tacttatcaa ttttacacat
    36841 gattttttgg tcattcgaga ctaaaaaccc taatcaaaaa cctccgtttt ttgcggaggt
    36901 tttgattgaa catctaaaag caagtttcac tcggtcacgc tttttcttcc caaatctgcg
    36961 cgatttcgat gatggttttt acggcggctt ccatgtcttg cacggctacc cactccagct
    37021 tggaatggaa actatgttca ccggcaaaaa tattggggca aggtaaaccc ataaacgaaa
    37081 gccttgatcc gtccgtgccg ccgcgaatcg gtgtttggat aggttcgatc ccgaggcgtt
    37141 tcatcgcttc gacggcatac gctccgactt gcggatgatg atcaagcact tctttcatat
    37201 tgcggtattg ctctttgact tcgcactcta cgcgggcgcc gggaaattgt gcggccgttt
    37261 tttcagcaag atctttgaga aaatcttctt tttctttgag ttttgccgtc acaaaatcac
    37321 gaatgataaa acgtatggtc gccaattctt cattggctgt aaacgatgtc ggatgaacaa
    37381 acccctcgcg cttctccgtt gtttctggag agagaccgac ctttggtaaa ttttcgataa
    37441 aatgagatgc cactttgatg gcattgacca tttttccttt agcatatccg gggtggatat
    37501 tttttccatg aaatcggatc gtcataccat cggcgctaaa cgtctcggtc tcgatttcac
    37561 cgcgactgga gccgtccacc gtataggcat atttggcgcc taattttttt aaatcaattt
    37621 tttccgtacc gcggcctact tcttcatccg gtgtaaaaca tactttgatc gggccgtgct
    37681 tgacttccgg atgagacaaa aaataattaa ccgcatccat gatttccgcc acaccggctt
    37741 tattatccgc gccgagtagc gtcgtaccat ccgtggtaat gatgtcaaag ccgatcattt
    37801 tttcaagatc agggttgttt tttacctcga taatttgatt ggaatcgttt ttgaggcgaa
    37861 tatcgccgcc ttggtaattt ttattgatca ccggattgac accggcaccg gtcactgccg
    37921 gcgatgtatc cacgtgtgca ataaatgcaa taggatccac gtttttggag gtatttccgg
    37981 gcaatgtcgc catcacataa ccatactcgt ccatgtgcgc atcggccaat cccatcgctt
    38041 tcaattcagc ggcaagatcg cgggataaat ttttttgttt ttcggtactt ggaaaagaag
    38101 tggactcttc atccgactgg gtatcgtatt tgacgtattt caaaaaacga tccacgcagg
    38161 taaatgtata cttgggatca aacatagttt tgcccttaat ggagttgtaa atagtattga
    38221 taaacaatgg tacctttttt tttgtaaaac atcaagatac ggcttacgat tgtacctatc
    38281 attctatgcg attatgtttt accgcttgat gttcatcgcg ggaaatttta tactgtgccc
    38341 ccacgtaacg aaactgcaag gaattatgga tcaacgatta aacgatctta cggatttggc
    38401 atacaacctc tggtggtcat ggaatcaaaa cgcgcaatcg gttttccgcg aattatcccc
    38461 gcaaatctgg gaggaaagca atcataacgc agttgcggtt ttgcacggaa cgagccacca
    38521 agaattgcgt gcacgtctca acgattcaca ttttcgtgaa aaggtggatc gtgttattac
    38581 gcagtttcgc agctatatgc ggcacgaaaa cacctgggga tcgcatcacg ctcaaacgtt
    38641 tcgtgataag ccggtcgcct atttcagtcc tgaatttgca ttgcatgaat gtttaccgat
    38701 ttattcaggc gggctcggcg tactctccgg cgatcatatc aagtcggcca gcgatttgga
    38761 tattccattt gtcggtgtcg gtttgtttta tcgtcagggt tattttcagc aacatatcgc
    38821 cattgacggt acacagcagg aactctaccc gctcaatgat acgacgcact tgcctttgtc
    38881 acttctttcc gaccgtacag gaaaacctgt agtaactgta gttgaaatcg gacacagtct
    38941 ggtcagctta tactgctggc aagtcaaggt cggtcgcgct caactgtatt tgctcgatag
    39001 caatcatccg gataacgaag aacattatcg cgaactaaca tcacgcacct acggcggtga
    39061 tattatgacg cgcatttgcc aggaaatcgt gatgggcatt ggcggtgttc gattgctccg
    39121 ttctatcgga attcgtccgt ccgtatatca catgaatgaa ggccatagcg catttctcac
    39181 actggaactg ctccgtgaac gtatgcttga aggccagccg atggatacgg cgcgggcatg
    39241 ggttcgtgaa cattgtgttt ttaccacgca tacgccggtt cctgcgggcc atgatcgttt
    39301 tagccgcgac ctgatgacgt ttgcattagc caactattgc gcgattcttg gattaacgat
    39361 tgatcaggtt atgggcctgg gacgcgtgca tccggacgat gaaaaagaaa cattttgtat
    39421 gaccgtactg gcactcaaaa tgtctcgcgc atccaatgcc gtcagcgcgt tgcacggtaa
    39481 tgtcagccgc gatatgtgga aagaactttt tccggatcgt cctgttgagc aagtgccgat
    39541 ttcacatatt accaacggta tccacatctt aagctggatg aataagcgca cccgtaattt
    39601 ttggcgcaaa tttctcggtg aaaactggga acaacatatc aaacaaccgg atttctggca
    39661 acgcgtagcc gatccttcgt ttatttccga tgaagaaatc tgggcgctgc gttatgatct
    39721 ccggcgcatg ctcatcgagt ttatgcgtgt acgcctcaaa gaacaacaac gtcgtgcggg
    39781 cggagacggt gtgcgcgtgt tctcaaactt tttttcgcca gatatattaa ccatcggttt
    39841 cgcgcgccgt tttgctacct ataaacgcgc acccatgatt gttcaaaacc tcgaacgggc
    39901 gattcaattt ttcaatgacc aggatcaacc gattcaggtt gtgttttcag gtaaagccca
    39961 ccccaaagac gacagcggaa aagcgttcat caaacgcatt gtcgaattga gcaaacatcc
    40021 ccaacttttt ggcaaactgg cctttatcga aaattacgat ctttatgttg cccggtttct
    40081 tacatccggc tgcgacgttt ggcttaataa tccccgtcgt cctcttgaag ccagcggtac
    40141 cagtgggcaa aaaatcggca tacacggcgg actcaattta tccattcttg acggatggtg
    40201 gcgtgaaggt tatgacggga ctaacggatt tgctatcggt aaggatgagt cgctgggcaa
    40261 tgaagcggaa caggatcgct tagacgaaga aaatctttat ctgacactca aaaatgaagt
    40321 gatacccgca ttctacaatc gcgatgccaa cggtattccc cgcgtgtgga tccaaaaaat
    40381 tcgccgcgcc atgcaaacct tgattccggt gtttaatacg gatcgtatgg tcgccgaata
    40441 ttttgaaacc atgtatcgtc gcaattcata acgacgacgc acgaagatat gcgagtacgc
    40501 gatccgcgtg aacttgcgca tccctttcgg catgaatcac gtccactatt tttccatccg
    40561 acggacgaat gatatacgtg actcttttat tgcgccagag tccccacaac cacagcgtat
    40621 caaacttttt gcttaatgtc cgatcatagt cgtaaatgag tgcgtacggt aaccgatgat
    40681 gtgtgcgaaa tttttcatgg gtcgcgtcgt catccaaact gacgccaaaa accacaccgc
    40741 cttgtgccgc gatttcgcca tatacatcgc gaaagtaaca cgcttgcccg gtacaaacgc
    40801 gtgtaaaatc catcggataa aaaaagaaaa ctacagcttt gtgccggcta tattgcgcaa
    40861 gcgtatgttc ctgaccgacc gaatcccgcc aagttaattc agaaataata gcattttttt
    40921 taagcataga gagattaact ccttttgttt ttttcgtctt gcaacacggc ctccgcgcat
    40981 cggataccgc taagtaccgc gctttcgatc gtcgaaggta attcatcggc gatccagtct
    41041 ccggctaaat acaacccttg aattccagtg cgactatgaa aacgatacga atctttcgca
    41101 tgattgttcg aggtggcgcg caccgacttg atcatttcga tacgacgaac cgaactaggt
    41161 ttaaaatttt taaacaggag tgtcatgtca tctgttattc tcttttccca aattgacgct
    41221 gcatcaaaaa cagacgcgcc gggatggctg atcgtaatct gaatacattc tgagtttttt
    41281 ttgaataccc attgtacgtg cgttcctata catgcggcca taggtccgtc gaatagcgca
    41341 tccggatgca tctcatttaa ccatatatag gccgataata tcggtgccga tgaaaaattt
    41401 tggagccgtg aagtccattc gttttctaca ggtaaaagct tgccggcact ccacggcggt
    41461 acggccataa tgactatatc tgtccgaatg gtttcgccgt ttcgcaacgt gattccttct
    41521 acccggccat tagaaacggt gatatgacgt acaaatgaat gaaaatgcaa cgaacaactt
    41581 ttttgtttta aaaaccttac agccggttcg accaatacgg cgtcgagtga tttttccgaa
    41641 ataataaaac gtccatcgtc gcgtgaaccg aaaaaaattc gtcgcatagc ctcgccaaac
    41701 tgaaccaaat cggcatcgtc gggatgctga ttcataatcg ccaaacaaat cgggttccaa
    41761 aaccatcgtc gtgtgtcctg cgtaaatcgc caggcgtcca accaatcgga tacataccgt
    41821 ccccttatta acgatttacg cattgataaa cccgctctca aaatacgcca gcgttcgctc
    41881 aatgggatcg catcgtatcg cataataccg tacagggcat accacggcgc aggccaatcc
    41941 ggtaaactca aacaggctat ccggccatcc gcggaataaa aattcaaagg aaacgatttt
    42001 ttgggaatat ccaatgaggc atttaatgaa tcaagccacg cacgggtagc atgataacac
    42061 cccatcagga gatgctggcc gttgtcgagt gtcgatcgtg atgcaacatc ggtaaaagaa
    42121 aaagtacgcc ctccggcata ggcatttttt tcatataaac ttacatcata ccccgcctgt
    42181 gcaagtgtcg tcgcggcagc cagtccggct gtaccggcgc caatgatgat tatttgagag
    42241 ggagatttag ccacggtaag ggcgatcatc aaaccaagtt cgacctgcgg tttgtatttt
    42301 ttccgataaa gtaaggctta ctcgatgagc ggtcggtgta aagccggttt gttcgatttt
    42361 atcaagcaat aaccgataca tattttccat gacccgtgcc ggaaaaagcg ccaacgacgg
    42421 atacgcacga aacgcttcat cggccaattg gaattgtttt ctcgcttcgg cgcattgtag
    42481 cctcaacgca tcctgaacgg ctgacggatt atcaaaaaac gcacgccgat ccgtcaccgg
    42541 aaaatagatc cgtccgtccg ctatatcaga tgcggcatca cgaatcgtat tggtcaattg
    42601 taaagctaaa ccggcatgca acgcaaaccg ttcactggcc gtatgatgcg ctcccaaaat
    42661 cctaatgatc atcaacccaa cggctgacgc aacgcaatag caatattcat acaattcatc
    42721 aaatgtgcgg tattctctgc gctctaaatc catttccatc ccgcgaataa cgtcatgaaa
    42781 caattgaaca ggaatcgaaa aatcacgcgc aacccgcatc acccgattga gcaaataata
    42841 tgacgaataa ccgcgctcgg cggcaatcaa ttcttttttc catacagaca aatgcgtcgc
    42901 ggcatcggaa ccattttgat ccacgatatc atccgtgatc cggcaaaatg catataccgt
    42961 aaaaatagct tcgcgtttcc ctcccggaaa gagcatcatc ggataataaa agctgctgcg
    43021 ggagcgtcgc gtaatgcttc gagcatcctc ttgcaatatc gtttctatat ccataaaacg
    43081 cgccatagta tccaaaattt gtcagttaaa ccaatcgtcg gacgatgatt cagcgtatca
    43141 ccgcccgttt tttccatttt cataagcaca cgtaatccgc tcaaccatat cataccgatg
    43201 attttgcgat ctttgaaacg caaatatcca agcaacgcat gaccttgatc aaacaaatca
    43261 cgtgtttttt ttaaacaaac cgccatcgtc tttgaaaacg acggatttac cgttttattt
    43321 ttccagtcct cttcggtata gccgcactct ttcatcagct ccagcggtat atacaaccgg
    43381 tttttctgaa gatcaatgaa tacatcctgc caatgattgg tgagctggag cgccgaacaa
    43441 atggcatccg acaacggggt cattttttcc cacatagtcg agttatattt gtagccaagg
    43501 atatgcagta cgatgaggcc gaccggattg gccgaataac gacaatatcc catgagttgc
    43561 tcttcgtcgg tatatctatt tttaagaaga tctactttaa aagcgctcaa aacatcatcc
    43621 aaccattgac ggggaatacc gcccttttgc aacgtcacgg acagcgccgc aaacatcgga
    43681 tctttggcgc gtccttcttc gctggctttg agcgcagcct gcatccataa aagtccgtgc
    43741 atacgttgtt cgatcgtgcc tgtgccttcg tcggctacat catcggcgcg gcgtaaaaag
    43801 gtataaaccg ccgcaaggtg acggcgttga gcggcattaa aaatccatga aataacaggg
    43861 aaattttcgt aatgcgaagc ggcaaaatcc cggcacatcg cataggccgc gtctatttca
    43921 tccacggaat acgaaccgat cgcccgctcc tcattcatcg cgttctcgca gtgttaggcc
    43981 gactacccaa agcgcgagta cgatattgaa tcctacgagt gcagaatacc ggcgatgcca
    44041 tgcgtcgaat tccttacgct cggggcgcgg atcattttct ttggcgtatt gatcgaagtc
    44101 tacgattttt tctttgagca tcgtcatctg cggcgtgata aaatccgcgt agaaaaaaag
    44161 attgatcagc atcacgccgg acacgacccc acgtacacgc gaccatagtg ttttttgacg
    44221 cctatacaga acaaaggacg atactacgat caacgccgcg cacaggtatt ccaaatgatt
    44281 cattcgaaca agaatcaatc cgttgatata tccggcgatg gttttggaat caaggtttct
    44341 aaaaatagta ccggcaacac cgtatgccaa aaacacgaga ataccgatcc atgcaccggc
    44401 aaacaataat atcgctccgc gccatgtata atttcggata ttcatgcgtt ggtactcccg
    44461 ttaaatcgcg agggtgaaaa gggcgccaac atcggcgaag atttgcctgt gatgatcata
    44521 tcggctataa gtttggccgt tatcggcata agaagaatgc cattgcgaaa atgccctgtt
    44581 gctacgatga ggttggtcaa ccgtgtttca ccaagcagcg gaaggcggtc cgcactacag
    44641 gggcgcaaac cggcccaccg ctctaccgat tcgaaagttt tggactgcgg gatcaatgct
    44701 cttagttttt cgataaatcc gacaatcacc gagggcgtca cagctttatc aaagcccgct
    44761 tcttcgacgg ttgagccgat aagcaaccga ccgctattgc gcggtacgta ataaaaatct
    44821 tcaaggtatg ccgggtatcg aataaaatca ggtttcggca tttgcatcgc gacgatctga
    44881 ccccgtatcg gcttgatctc cggtatcgtt tgtgtcaatc cgggaatctg tgaagaccat
    44941 gcaccggcgg ccaatacata tcgtgccgct gataccgtcc cggttttagt ttgaagatga
    45001 tcaatcgctt cattgttgag aatgatggat tcgaccgcag aatactccaa gatatccacg
    45061 ccgagatttt tgcagcattc ggccagtgcg gcgctcaatt tgcggttttc cacctgatgg
    45121 ttatccggca aatgaatcgc cgccatgcct tcgcgcaatg caggctccgc ttcgccggtt
    45181 tgttccacac tccaaaaatg cgcatgcatt ccgagcccga cataccacga caaccacggc
    45241 ttgggatccg tttcaggcga aattagtttg agaatgcctt ctttgcggta ttcgcaatcc
    45301 atacggctat gatattcgat acgccgaatg gtctcttcat acatgttttg actttccctg
    45361 cacaatcgaa aaaaagcttt ttcgcgatcc gacaaatttt gacgcggcat gatcaactgc
    45421 ggttcgagca tgcctgcgga tgcgtgggaa gcctcatatc ccgtttgtgt tttttctaaa
    45481 atggctactt ttattttgtt gtgacgaaat ttgatttcat acgcgatcga aagcccgatc
    45541 acacccccgc cgatcacaac gacatcatat attttcaaaa ctaactcctg gttgatgctg
    45601 acaattcaat atagaagcgt tgtcttaaaa aatcaagctt gggcgcgtac tgaaacgctt
    45661 aattttcaat ggttttaggt gttttttatg cttgatattg gcttagccaa gaaatatatt
    45721 gccgcgttgt aatccgcaat tcttttatct atttattgta attttaaata tacaattccg
    45781 ggagtttttt agtggaaccg aaaaagcttg gccgttatga aatcgtacaa gtcctgggtc
    45841 agggcgcgat gggcattgtg tacaaaggcg tagatccgat gatcggacgc acagtcgctc
    45901 tcaaaacact taagtccggg agcgaaattc cggaacagca gcttacagaa ttcaagcgcc
    45961 gttttgcgca agaagcacag tccgcgggcc gtctcaatca tcgtaatatt gtgaccgtat
    46021 atgacgtcgg cgaagaagaa ggtttggcct acatcgcgat ggaattcatc aaaggccgtc
    46081 ctttggatga cctgatcgcc gaaaaacatc cctttaccat agagcaaatc gttgacatca
    46141 tgatacagat ttgcgaaggg ctcggatacg cgcataaaaa cggtgttatc caccgcgaca
    46201 tcaaaccggc gaatatagtt ctcaccgacg atcagatcgc caaagtaacc gatttcggca
    46261 ttgccaaact cacttccacc agtgcgacac aaaccggcat ggtcgtcggc acgccgagct
    46321 acatgtcgcc tgaacaaatc accgggcgcg gcgtggataa tcgttccgat attttcagta
    46381 tcggcgcggt gttttatgaa ttactcactt acgaaaaagc ctttcccggc gataatatta
    46441 ccacagtcat gtatcgtgtc gtgcatgaaa atcctgcgcc gctatccatc agcaatgcga
    46501 tggtgcctcc gcaatttcaa gcaatcatcg aacgggcgat ggctaaaaat ccggcggatc
    46561 gttatcctga cgccgaaacc atggccagcg atattcgcaa ctacaaaaca gctacggcca
    46621 acatgggcgc aacgcaagtg atcaatcctg ctaactatgc cggcgcaacc gtcgttatgt
    46681 ccgctccgga tttttctgcg atgtcaccta ccggcacagg atccatgccg tcccctgctg
    46741 cggcgcagac gcagtccata cctgccgcat caccggcttc caaaaaatca ccggtggcac
    46801 ttatcgccgg cggcgctgtc gctgcgatct tgattgtgct atacatgatg ttcggcggct
    46861 ccggtgataa atcatcggct tcttcagcta aatcttccgg cacagggaat gcatcgctta
    46921 cgctaacact caacgcggcg gaaggcacgg ccatgctgga ttccgtgcat gtgccgatca
    46981 aagatgccaa aatttctgca gaaaaaatca ccgccggtga gcatgaacta gttattcaat
    47041 ccgacggtta cgaaacgtat cgttctaaac ttctttttgc tgacggagaa accaagcagt
    47101 ttgccgtaga actcaaactc gcaccggtaa aatttccggc cggtgtagat acggcataca
    47161 tcactgtttc ttcgacggac atgatcaaag tgatgaccaa tacgggaacg atgttaggct
    47221 attcgccgat tcaacgttta ccgtttccgt ccggaaaaca cacactcgtt ttttccaagc
    47281 cgttttatac caatcaggtt tcggaaattg acgcccgcaa aggcaaagag attaaactga
    47341 cacccaaact gcaagccaaa cgcgtcaaag ccaatcttgc cggggtagaa cctaaaaaaa
    47401 ttcaggtata tctgaattcc gtagcctctg aaagtcagtt gaagccggag aaagacggtg
    47461 tgacgtattc cctaccactg ggtactaata aactaatcgt tacggccgac ggctacaaaa
    47521 gttatgaaca ggaacttaat ctcggcgacg atgcaatacc ctttgatgtc aaagccacgc
    47581 tggtacaagg tttcggatcg ctcgcgatca cttccaaacc ggacggcgcg gatgtatatg
    47641 tagacggaaa aaaagaaggg gttaccccat tcaaaatgga taaaattcct gccggaccgc
    47701 gtgatatcaa agttcaaaaa ggctcgctgc tcagtatcaa aaaaatgaat atcgaagccg
    47761 ataaagaaaa tgcagccgac tttacactcg ccgcatccac gggcattttg aaaatcttaa
    47821 tcaatccctg gggcaatata tcggtggacg gtaaaccgat gggcgcttct ccgccgctcg
    47881 aacgtctcga attgagtccg ggtaatcata cgatcaaaat cgaaaacccg gcgtacaaag
    47941 ccgtcactaa acaagtaaaa atcaccgtag gccaaaccac tacgttacaa tatgatttct
    48001 aatatgaaaa aacattttag cctcatcaca ctccttttcg ccggaataat aatttttacc
    48061 ggttgcggcg gatcaaaaaa cacgaccaaa gaaaaacccg aaacaccggc cgttcccaaa
    48121 aagcccagcg gtaagccgca gttggaaaaa ggaatggaac tatacaaagc ccgggattac
    48181 gaaggcgcta aaaaaaatct gcgaagcgct accgatcttg atttatccaa aagcgagcgt
    48241 acccaaacgt ataaacatct cggtttcata tttgcgatgc aaaaaaatca aacggaagcc
    48301 actaaagcat tcttcgacgc atttacaagt gatggcgaat ttgaattgga atccgctgaa
    48361 atgggtaatc cggcctggac accggcttac gaagcggcac aaaaacaatt tacgttagcg
    48421 agcgcctcgg gcagcgatct gctgagtaaa ggcaaagatg cttattctaa acgtaactac
    48481 gatgaagcgc tatcgtattt ggaaacagcg gttaaaaaaa ctgatctcga aggcaacaaa
    48541 cgtgcggatg cttacaaact attggctttt gcctatgctc tccgtaagcg ccccgtagat
    48601 gctaaaaacg catttcgcaa agcctttgaa gccaataaga atttcgagtt ggataaatcc
    48661 gaatacggaa atccggtttg gacgccgctt tatgatgaaa taaaaaacac tatcaaaaaa
    48721 taatacagat tggacgtacg ggtggcttct ctcgttctca tcgaaaataa cacaaccgca
    48781 acacgctttc gtatcgaaaa agacgatacg atcataggac gccgaccgga aaacgatatc
    48841 atgatcgcca acgtgatgat ttccggcaaa catgcccgtg ttgaaaaaaa gaacggcttg
    48901 tatcagatca ccgatctcgg ctccacgaac ggcacatttg tcaacggagc acgggttacc
    48961 ggttccgctg aaattaagaa tcaggatcat atcaatttcg gcgccgtcga gctcattttt
    49021 atgacggatg aaaatgcgat ggtcaatatt gaccggacgc gtttttactc ccgggacaaa
    49081 gctgtcgcgc cgccgcaaaa accccatgac acgggctatg cggccaatcc caatgccgac
    49141 aagatcgtgc aacttttcca acagctcaaa aataccgtgc tcagcggttc ggcggatcgt
    49201 acggccatca tgactcagtt tatggaggtc gaacatcaac tgcagatcat gaatatgcag
    49261 ttcaaagaat ccgaaaaatc gcagcaaaaa ctcaatgtgc tctacgagat cgggaaagtc
    49321 atcaaccaca ttctcgttga agaagaattg ctgaaaacga tcattgattt agccctcaaa
    49381 gtgatgaacg gcgactgcgg atttatcatg ttgtacgatg ataaaaacaa tcttgtccca
    49441 cgcgtttcgc gcaaaatgca ggccgacgaa atttcccaat ccggttcgac gttcagttcg
    49501 acgatcgcca aacaagtggt cgaaagcaat caatccattc tgacttccga cgccaaaagc
    49561 gattcgcgct ttcagagcgg cgccagtatt atatccaaca acattcgctc cgttatctgt
    49621 tcaccgatgc gcaataaaga tcaggctgtc atcggcgtga tctacgtcgg ctccaatgtc
    49681 atgaccaacg tattttcaaa aagcgacgtt gaattgcttg aggctttcgc caatcacgcg
    49741 gccatctcca tcgaaaacgc caaaatgcat gaagaacgcc gtcgcaaaga gcacctcaaa
    49801 agcgccttgg aacgttatgt gtccaaacaa attgccgaac gtatcatgag taacgacggc
    49861 agttctattc gttttacgcc cgaacgccgc gaagtcacgc tcattttttc cgacgtacgc
    49921 ggtttcacta ccctatcgga aaaattatct gccgaagaaa tggttgaaat acttaatcgg
    49981 tatttttcgc gtatgattga tgtgattttc aaatacggcg gcacgttgga taaatttatc
    50041 ggcgactcca ttatggcgct tttcggagct ccggccactt ccggcgatga tgcatggaat
    50101 gccgtgcagg ctgcgataga gatgcaacgc ggtttggagg catttaacga cgaacagcgt
    50161 caattgggca aacccgaaat tcaagttggc atcggaatca acaccggcca cgtcgtcgtc
    50221 ggcaacatcg gatccgatca gcgcatggaa tacacggcta tcggcgataa tgtcaatctg
    50281 gcatcacgtc ttcaaggcaa agcggccgga ggaaaaataa tcatcagcaa agctacttat
    50341 gattttgtca aagataaggt gcaagccact cttctcggaa caaccgaagt caaaggcaaa
    50401 accatacctg tcgaaattta cgaagtgatg tattgatatc ttcgtaaagt ctaaactata
    50461 tcgccatcta tttgaaagaa actatatatg gatcttaata cgctgattta cgattggaat
    50521 caagaagtca acgctgaaga aaaacccgcg catgctttcg aactcaacga tgaaactttg
    50581 cgtgacggtt tacaatcgcc ttccgtaaaa acgcctacac ttgagcaaaa aattgaaatc
    50641 ttgcacctca tggagggact cggtattcat gccgccgaca tcggtttgcc cggcgccggc
    50701 cctcatgtac tcgaacacgt tgttgctttg gccgccgaaa tcgcaaaaaa taaatttaaa
    50761 atccaaccta attgcgcagc acgtacactc aaggttgata ttgatccgat cattgaggca
    50821 tcccaacgca ccggtatccc cattcaggcg tgcacgttta tcggctcgag ccccattcgc
    50881 cagtacgccg aaggttgggg catggatcaa cttttgaaat ttacggaaga ggccgtttca
    50941 tacgccgtga aaaacaatct ggatgtgatg tacgttacgg aggacacgac ccgtgctcat
    51001 cccaacgatg ttaaacgcct gtaccttgcc gcgatacgaa gcggtgcacg gcgcgtttgt
    51061 atctccgata ccgtcggcaa tgcaaccccc gaaggtgtac agcgccttgt acgttttatg
    51121 gtggaagtaa tcaaagaaag cggcgaagat gtaaaaatag attggcatgg ccacaatgac
    51181 cgcgggcttg ccgtcgctaa cgcaatcatc gcattacaag ccggagccca tcgcgtccac
    51241 ggaacgggca tcggtgtcgg cgaacgcgtc ggcaatgtgc aaatggatca actcatggtc
    51301 aatctcaagt tgaaaaactg gataaccaac gatcttacta ctctgccgca atattgccgt
    51361 aaaatttcgg aatacacggg cataccgatt cccatcaatt atcccgtttt cggtcacgat
    51421 gcatttcgca ccggtacggg agttcatgcc gcggcgatcc tgaaatccct caataaaggt
    51481 gacatggaat tggtggatcg cgtgtactcc agcgtaccgg cggctatgtt tggcctcgaa
    51541 caagtcgtag aaataggccc gttgagcggt gagtccaatg tggttttttg gctcaaacgc
    51601 cgcaagatcg aaccgcgtga agaattggtt aaaaaaatat tcgctgcggc aaaacaatcg
    51661 caaaccgttt tgagtgacga acagattttt tcgctttgcc gataaaggtt ggggatgatt
    51721 ttgtctcgcg taagtctttt tatttttttg ataagcaaca cgctttgggg tcaaacgacc
    51781 gaaaagacgt attggatctt ttttaaagat aaaccagact acctgcacgc cacggtcata
    51841 ccggatattt cgccacgggc tttggaacgg cggctaaaac aaaatccgca cacgtccgcg
    51901 ctgatcgacg aatccgatta ccctgtttct tcggattacc ttgcggcttt atcctctcat
    51961 cttgtattga ttcataacac atcacgttgg ctcaatgcgg cttgtgtgca aatcaatgaa
    52021 agaaacatcc cccttattca atcgcttccg tttatccgcg aaattcgtcc ggttagagcc
    52081 cgatcggtga tagcgtcaac ttcttcacat gcatcatctt tttcgtctat cgcagcctcg
    52141 tttagtcgct tttcgtatgg cgcgtcatta cggcaaaatg catttgtcaa tgccgttgcc
    52201 gctcatgact ccggttattc agggaaagga gttcgcatcg gcatgattga tacgggtttt
    52261 aatctcaccc acacggcttt ttcaggaatt cacgtcgtag cagattccga ttttgtggat
    52321 cacgacggta acgtgagcga aatcggttcc aaatttacca gcagtcatgg gacatttgta
    52381 ctttctgcaa ttgcaagttt tcacgaaagt tttttgatcg gtacggcttt cggtgcggat
    52441 tatatattag ctcgtactga aaataatagt gatgcaatct atgaattggc ggacgaatca
    52501 cgctggattg ccgctttgga atggatggaa caaatcgggg cggatatagt ttccagttca
    52561 accagttttt ttgacgaatt tccaggcact tcaaatgatt acaccatacg cgatctagac
    52621 ggaaaaaccg gcttgactac acttgctact gatattgcat tcaagaaagg cctgctcgta
    52681 tttaatagtt ccggaaataa tggcgctgaa gatcaagatc aatatcgcgt cagcacgcca
    52741 tccgacggga agcatatgat cgctgtcggt gcgacgatcg gcgacagcac ggtagccagt
    52801 tttagttcgc gtgggcctac ggctgacggt cgaaaaaaac cggatgtcgc agcgttgggc
    52861 tacgagatac gcgtagtttc cagcggaaca acgggttacc aaaatttttc gggcacttcg
    52921 ctatcaaccc ctattgtagc cggaattgcc gccttagctt tggaagcaaa cccggcctgg
    52981 tccggttcgg aattatatga tgccgtccga aaaacggcca gtttatcaaa aaaacctaac
    53041 catactatcg gctatggtgt gcctgatgcg atgaagataa ttcgttataa ccccgcttcg
    53101 tccgatgatt ttaatccgat acgcgatgtc gtcaattatc ccaatccggc caacccatcc
    53161 actactatac gttttcgatc cgttataagc ggacgttatt ctgtacatat ctataatacg
    53221 cttggtgcat tggtgcgcac gatacgggac aatgcgtcta tcacggcggg agaaacggta
    53281 gcggcgttat gggacttaaa aaatgatcac ggacggaggg tcagttcggg agtatatatt
    53341 taccgcattg caatggatga caaatcagaa acacgaaaaa ttcttattat aaaatagaag
    53401 catttttgtt tttttctact atccgctgaa gcgccttcat cactgatgga atatcttgtt
    53461 cagaattcaa acgcccgaat gtgaaacgca ccgcactccc gtaaaattca ggacctaacc
    53521 ccatcgcctt gagcacatgc gacccgctta cggcaccgga tgtgcaagcc gatcccgtag
    53581 aaacggaaat accttccaaa tccaacatca tagctatttc atctcctccg atcccgggaa
    53641 acgaaatatt aaaatgtccc ggcaatcgtt tttcaggatg accgttcaac cgggcttgcg
    53701 gtgcaatatc ttgcagagtt ttccaaaata ctgtcattag ttttttcaga cgttctgatt
    53761 caccggacat actgtctttg cagatctctg cggctttgcc aaatccgacg atacccggca
    53821 cattttccgt gcctgcacgt tgcccacgct cttgtgaacc gccttcgata aatgaaggta
    53881 tttttacgcc acgccggata taaagacagc ccacaccttt ggggccgtat attttatggg
    53941 cggagacaga taacaaatcc actggtaaat tttttacatc gaacggtatt tttccaaaac
    54001 tttgcaccgc atcggtatgg aaaagaattt gatgcgattt ggctaaagcg cttatttctt
    54061 cgatcggatt gatcgtacct acttcattat tgatgtggat gacagatatg aatacggtat
    54121 cgggacgaat ggcctgtgct atagtttctg cggacacgta tccgaactga tcgcatggaa
    54181 cataggacac agcataaccc gatttttcca acgattcgaa cgtatgcagg acggcatggt
    54241 gttccgttcg tgtcgtaata atatgtttac ccttggcact cattgatttt actgcgccga
    54301 caatggccat attgtcggat tcggagccgc cgccggtaaa aacaatctct gaaggttgtg
    54361 cctttattac cgacgcaact ttttcgcgcg cgtcttcgat agccacttta gccgagcgcc
    54421 ctagtccgta aatactggat ggattaccga attcttcggt gaaaaaaggg agcatcgcat
    54481 caacgacccg cttgtctacc ggtgttgtcg cgctgtgatc cagatagata cgtgctttgg
    54541 ttttttgcat attatttgtg aatgtgaata cgcggtacac gtgcgttgat catacaggta
    54601 atttcgtaag gaatcgaacc gattttttca gctaactcca tcgccgtaat ttcttcattg
    54661 cccgaacgcc cgatcagaat gacttcatcc cctatttcaa cacgatcatc gccgatattg
    54721 accatcaact ggtccatgca caccgcaccg accacaggat accttttacc gcgaatcaac
    54781 acatcggctt tattcgtcaa aagacgtgta tatccgtcgc cgtatccgat cggaaccgta
    54841 acgatattgc aatcctgagg cgttatatac ttgtggccgt agctgatgcc cgtaccggcc
    54901 ggcacacgtt tgataaaaac aactttagat tttaaagaaa gcgccggctg aagctgtata
    54961 tcgcgacgaa caaaacgcga aggatacatg ccgtataacg aaatgcccgg acgcaccatg
    55021 tccacgtacg tagaatcaag gttcatcacg gctccgctat tggcaatgtg cttgattggg
    55081 atttcaatac ctaatttttt caatgcatct atgatttcgc aatatcgttc aaactgcgta
    55141 tgcgcaaaaa aaaggttttc ttcatccgcc gtcgcaaaat gagaatagag acctacaatc
    55201 tcaatatgcg gaagcatggc cacacgtcgt acgaactcta ccgcttgttt gtatgtcaca
    55261 ccgattcgtt ccatgccggt atctatttta agatgaaccc gcgctttttg atttttaatt
    55321 tcgtcgttga tatgatgcgc cagttccaaa gaagaaacag tgatttcgag atcgttttga
    55381 acaaattttt taacctggta acctagaata ccgcccatca ccaggatagg cgcttcgata
    55441 tcctcttgcc gtaaaaaaag cgcctcctcg agaaagccca cacctatgta gtccgccccg
    55501 ctccgcacgg ctttttgcgt cacttcgcag ataccgtgac cgtaagcgtt agccttgacg
    55561 atagccatga tttttcggct gccgacccgg gttctgatct gtgccatatt gtgtgcgatc
    55621 gctgccaaat caatcaccgc atgcgtaggg cgaatttctt ttagtatatt gtggtatatc
    55681 gtatccattg tttaaccgat ctggccgact acatccgtta tcaactgctg aaactgtgtt
    55741 ttgacgcggt tggccgtctc cgtcacttct tcatgactca gtttggtttg actgataccc
    55801 gtaccaagat tggtaataca actgatcccc agcacgcgca tattctgatg ttttgcgacg
    55861 attacttccg gcaccgtgga cataccgaca gcatcggctc cgattttttg gaacattttc
    55921 acttccgcgc gtgtttcgta tgtcggtccg gaccccgcac aatagacacc gcgttgggta
    55981 cgaacaccca acttcaacgc tgttttttcg gctatagata tatattcacg atcgtattcg
    56041 ctttccatat ccggaaatcg tggccctatc gtttcatcgt ttgggccttg aagcggattg
    56101 gcaaagaaaa aattgatatg acttgtaatg aacatcagat cacccggctg aaaaagcgga
    56161 ttgactccgc cggccgcatt ggtaacgatc agcgaacgaa ttcccagcat tcccatcaag
    56221 cgcacgacat atgttacttg cgccatcgtg taaccttcgt aataatgcac gcggcccttt
    56281 acggctaata cgtgtttacc gtttaacgta ccgaaaacca atttcccgtc gtgcccctcg
    56341 actgtggatt tggcatagtg cggtatgtcg gccgttgaaa tcaccgtttt attttcaaaa
    56401 cgatccgcca gatcaccgag accggagccg agaataatgc cgatcgtcgg tcgcatcgtc
    56461 gtttttgagc gaattatggc gatggactct tccatcattt tttttaattc atgcatacga
    56521 acctcttttg caatcgtggt acgccatcat ttaacaaacc tattatttat accgataccc
    56581 gtccctcgag cgcacgtgtc aatgtcgtca tgtcggcaaa ttccaaatcg ctgccgatcg
    56641 gtattcctcg ggcaatccgc gtcaaccgca cagatttacc ctgcatcttt tcggccacaa
    56701 acatcaccgt cgcatcgccg ggtacggtcg gattgatcgc taatatcatt tcacgcactt
    56761 cgttttcgga gcgctgtatc agttcgcgaa tgcgtaaatc atcagggccg acaccctcta
    56821 aaggattgag caagccgccc aacacgtgat acacgccacg aaacatatcc gttcgttcga
    56881 tagccaatac atcattggct tcttcgacga cacagatcac accgtggtca cggcgcggat
    56941 tggcacaaat cgcgcaaggg ttgtgttctg ttacattgaa gcatgtcgtg cagcggccga
    57001 tcttttcttt catgtccagc aaggcctgcg agagcataac cacttcgtct ttaggttgct
    57061 tgagcagatg aaaaaccagg cgttgcgcgg ttttacgacc gataccgggc aacttggaaa
    57121 attctttgat caaacgttcg atggtttctg acgtgtattg catgatcttt tatttttttg
    57181 gcattttcat gcccggcatc atgccaccgg tgattttgct gatttccgca ttggacatct
    57241 cgtcggcttt ttgtactgca ttgtgaatcg ccgataaaat catgtcttcg agaagtccta
    57301 catcatcagg attgacggcc tctttttgta tttttacgga caatactttg ccgtttccgt
    57361 tgacggtcac cgtaaccata ccgccgccgg aagtaccctc gacattaacc tgttcgagtt
    57421 ccgattggcg gctttccaat tgtttttgca ttttctgcgc ttctttcatc aggctctgca
    57481 tatcaaatcc acgcatacga tctccataaa tgattaagat gtagtaaggg tgtctttatt
    57541 ggttatgtct atcaattcgc aatccaaaag ttcgatcatg cgtttgagcg gcatttccgc
    57601 ttctttatca cgcacatatt tgaataaatc cgaacgtacc tgcacactga tctgcgcttc
    57661 gttgccgtcc ggttttacat cggcctcggt aaacggttct tgtgccgcat ccaacgtcgg
    57721 ataatgatca aagaaatttt cggccggcga cggctcaggt accggctcat cttgataggc
    57781 tacgcgctct accgggcgaa tgattttttc cggctcgaga accaaacgaa cacgtttcgc
    57841 tgtgaattga gcgatcacac cggctacaaa ttcacgttct ttttgtacca tttcgacctg
    57901 aaacggtgtc acgccgctcg tggtaaaacc caatttgagt aaatcgcttt gcaattcgta
    57961 aggccaggcc aactccaata cggacccgac gcgacgcttc tcgcggttga cttcgacgac
    58021 aatttttgac caagatttac gaatttcgct taatcctcgt tcaccgttta tcggtggttc
    58081 tttgggcggt tccgtgacaa ctaccggcgt tggtatttcg gaatgtttga cggctggcgg
    58141 tttaagaggc gttacaggat ttgcgggagc cgttacggca ctactttttt ttttacggtc
    58201 tccgtatcgg ggttctccag ttttcggatg atctccgaaa gttgaacaga atcttccata
    58261 cgaataagct tcatcagcat caattcgaat acaaagcgtt gcttgaggct ccatttgacg
    58321 cgttgaatgg tctccgatac caaattcatc atgcgaataa ggtcggtttc agaaaattgc
    58381 tgcgatacgt ctgtaaattt ttcgtgataa ctctcataca ccgtcgtcaa tgccgatgcg
    58441 ccacaggcct tggccagcag tacattgcgt atatgcgcca tcaatccgtg cagaaactcc
    58501 gtcacatcaa gacctgcatc cagtacgcgc gaggcaaatt tgaatccttc ggaaacactt
    58561 ttattgcgta tgatcgccat cagttcaaaa tataaccctg tttcgatcag cccaagcgcc
    58621 tcacgcacgc gttggatacg aacctgatta ccgcaaaagg aaatcacctg atccagcaag
    58681 gtcagcgcgt cacgcatacc gccgtccgcc atttgggcga tttcaaacag tgattcttcg
    58741 tccatatcaa tttgttcgat acggcagata tgtttgagtc gtccgatgat tttttcgata
    58801 gcgacgcgtt taaaatcgaa gcgctggcaa cgtgataaga tcgtcggcag gattttgtgg
    58861 ggttcggtcg tcgcaaaaat aaatacgacc tgtggtggcg gttcttcgag tgttttcagg
    58921 agcgcattaa acgcatcctt ggtgatcatg tgaaattcgt caatgatata aatcttgtat
    58981 ccgccgcgta ccggcaaata ccgtacattg tcacgaatac tgcgaatctc atcaataccg
    59041 cgattcgaag caccgtcaat ttccaacaca tccaaactgc gtccgccggt aatttcgagg
    59101 caggactggc attgattgca cggcgtgatt gtcgggccgc cattttcaca gttgatggct
    59161 ttagcaaaaa tacgcgccga cgttgttttg ccgacaccgc gcggaccgga aaagatatat
    59221 gcatgcgcga tgcgcttaga ttcaatcgca tttttcagcg tcgtggtgat atgttcctga
    59281 ccgatcacat catcaaaaac catcggccga tattttcgcg caattacaag ataggacata
    59341 gatatttggt taaaatttaa tgccggccaa gataaaagat tttgggaata aaaaaaatgg
    59401 aattttatac gtaagaagtt ctttataaag ctgttcgaaa caacacgaat tagcgactaa
    59461 ttatcatttt ctatttagat attcagcgtc aaaaaaatta aaaaaaagcc caagctgtta
    59521 cggcacatcc aagggtgagc ttaccgttgc ttccttccgg acctggcggg gttcagcctc
    59581 ctcaaattgc gtaaggctcg ggcgttaata aaacttattt caaaacaaca tgcgttcaac
    59641 gtcaatataa cataacgtcg taaacaataa aaccccgcct aggtagcgag gtttttcgtg
    59701 ggcagtgaag gatttgaacc tccgtagagc atagctcgac agatttacag tctgttgcga
    59761 ttgaccactc cgccaactgc ccaatgaact tttactgcat aagtagagct ggtgggcgga
    59821 tttgaaccgt cgacctactg attacaagtc agttgctcta ccagctgagc tacaccagca
    59881 atatctctaa tgtctaagaa cattctcaaa attcgggcga aaatatagat gatgccgcac
    59941 actttgtcaa tgaatatttt atgtatttca tgaattcttc gccatatcta accctgatgc
    60001 ttgacattca atcctgtttt gtttaccatg acgccacact atgaaactgt atttgttcta
    60061 tcccgctccc ctcttcctga tatgagtgaa atatcacgca tcgaaatttt actctttttt
    60121 ctgcttattt tcatcggcgg attcttcagc agttttgaaa cggccatttt atcgttagat
    60181 aagctgtcat tcaaaaaaat tttgaaacgc ggcggcacgc gctccaaact acttgagctc
    60241 tggacatcca atcaaaatga ttttttaacg ggtatctacg taggtaataa cgcatttaat
    60301 gtcggcgccg ccgtattggc cgccagtgtc agtgtggata ttgcgcgact gtacggctac
    60361 cgcgaagctt acgctttggc cgtggccacc ttccttatta cactttttct tctgttattt
    60421 agcgaagtat tgcccaaatc catcgcgcgc cataatccgg aaaaaacggt tatgctgttg
    60481 gcgacgccgc tgcatattgc gttactgatc ctcaaaccct tcagcggatt tttttcatgg
    60541 gtttctcgtg tcgtcgtcac cgcaacgggc ggtgaaacac atcgccgcag cctcaatgta
    60601 accgaagatg aaattcgcga aatcatcaat gccggcgaag tggcgggtgc gatcgaacac
    60661 aacgaacgtg agatgattca cagcattatc gaattcggtg atacgctggt caaagaagtc
    60721 atggtgcctc gcgtggatat ggtatgtgtg gaagtccaga caccgatgga agaaattctc
    60781 caaatcatgg cggatgaaaa actgtcccgc atccctgtct atgaagaaaa tatggatacc
    60841 gtcgtcggtg ttctgcatat caaaaacgtg atgaattttt ggcgtaagaa cattcaggat
    60901 atgacagcta ttgaattcat caccatgccc tattttgttc cggaaaccaa aaaaatttca
    60961 gaattattgc gcgaatttca atcgcaacgt atccagatgg cgatcgtagt ggatgaatac
    61021 ggcggaacgg ccggtttggt gacgatcgaa gatttgatcg aagaaatcgt cggtgaaatc
    61081 aaagatgaat acgatgataa tgtttcactg ataaaaaaac aggaagacgg ctcctatctc
    61141 gccgatgcaa aaatcgaaat ttatctcatt aatgaacaat tgcatttaca tctgccttca
    61201 gacgaataca gcaccctcag cgggttgatc ctttggcttt tcaaacgcat gcccaaaaaa
    61261 ggtgatacca tctcctatga cgatgttcga tttacgatca tcgaatcgga ccgaaagcgc
    61321 atccacaagg tactgataca aataccgtcg cccccatagt tccatcgacg acccaaaccg
    61381 ggataaatca tttgaataat cagttccact gtattacatt gcgtcgcttt tttaaacgac
    61441 ttttacgata tgatcaccat ttacgcgcaa ttgttcggca aagaatttgt caatacgctt
    61501 acgtttgacg aattggtgat ttatctcaaa gacccgcaat gtattttctg gatagatctc
    61561 aattccccca cattagacga aattcatcaa attcaggatg ctttcaattt tcatccgttg
    61621 tgcatcgaag atgtgatgag ttattccaat tcgcctaaat tggacgaatt tgacgaatac
    61681 attttcctcg taacacacga accccgaatg catccggaaa ccaatgaaat cgagcgcccg
    61741 gaaattgatt ttttcctggg taaaaattat ctcgtttccg ttcatcacga accaagcccg
    61801 gctatcgcca aatctataca ccgatgcgaa acgcaattgt tgtttcatca ggctgcacta
    61861 tatgaaaaag gttcgcgtgg tcgaacgacg atcaaagata actttatgtt taaaaattca
    61921 gattttattt tacatacgat cttagatcac atcgtggatg attattttcc tttggttgaa
    61981 aaatgggaag acgatattga tcagctcgaa gaacatgttc tgtccgttcg cgccgaacgt
    62041 tcggtgctca acgaaatttt aaaactcaag cgacagttgg ccggttttcg ccgcactgta
    62101 tcccctcagc gggatgtact ttcacgcctt attcacctcc aacatccggc gatgtccaaa
    62161 gcctcggtag tctattttcg cgatattttt gaccacctta ttcgtgtcaa cgaactgatt
    62221 gatacctacc gtgatacgat gagcaacgtt cttgatgcct actattccgt gctctcgcat
    62281 cagatcaacg aaaattcgca tattatcaat atcatcatga agcgacttac gatcatcact
    62341 accattttta tgccgctgac ttttattgcc ggtatttatg gcatgaattt tcataatatg
    62401 cctgaaatac ggtgggaata cggttattat tacgcactcg gactgatggg cgcactggcg
    62461 ctttccatgt tttatttttt cagaaaaaaa ttctggttct aacatataca ggaataccat
    62521 gcacacgact aaagccatcg aacttcatga aattcgcgag cccgaagtaa cacacaaact
    62581 tgccaaagat cacggcctca cggatgaaga atatgagcgc atccaacaaa ttttaggacg
    62641 aaaacccacg tttaccgaac tcggtatttt tagtgtgatg tggagtgaac actgcagcta
    62701 taagaattcc attctgatcc ttaaaacact acccaaacaa ggtcaattta tactcaaagg
    62761 cgcgggcgaa gaaaatgccg ggcttataga tatcggtgac ggtttagccg tggctttcaa
    62821 aatcgaaagc cataatcacc cgtcggctat cgaacctttt caaggtgcgg cgaccggtgt
    62881 tggcgggatt cttcgcgata tttttacgat gggtgcgcgt cctatcgccg ctctcaattc
    62941 tctgcgcttc gggcatctgg atgatgagcg cgtacgctat ctttttgcca atgttgtcaa
    63001 aggtatcggc cattacggta attgtttcgg cgttcctacc gtcggaggcg aagtatattt
    63061 tgaagaatgc tataccgata atccgttggt caatgcgatg gccgttggca tcgtgcgtca
    63121 cgatcagacc gccagcgcca tagccaaagg cgaaggcaac cccgtcatga tcgtaggctc
    63181 cgcaacagga cgcgacggta ttcatggcgc gaccttcgca tcagaagaaa tatccgaagc
    63241 gtccgaagcc aaacgcccga acgtgcaaat cggtgatccg tttacggaaa aactattact
    63301 tgaagcaact ctggaagcca ttcagtcagg tcatcttgtg gggattcaag acatgggcgc
    63361 cgccggtttg acctgttcca gttccgaaac cagcgctaaa ggcgaatccg gcatcgaaat
    63421 caatctcgaa ctggtaccga cccgcgaaga taaaatgacg ccgtatgaga tcatgttatc
    63481 cgaaagccag gaacgcatgc tgctgatcgt caaaaaaggg tatgaaaaag aaattcagaa
    63541 tattttcgaa aagtgggact tgcacgcggt cacgatcggt catgtgacca acgacaaact
    63601 gatgcgcgta aaatacaaag gccgtgtatt tgccgaaatt ccggcggata cgcttgtcct
    63661 cggcggcggc gcgccggtgt acaaacgcga aagcgcggaa ccggcttatc ttaaagaaac
    63721 gcgcgcgttg gatatcgcat cactacccca accttccgat cttacatcgg ttcttacacg
    63781 gctgctgagc acgcctaata ttgcaagcaa agaatgggtc tatgaacagt atgactctat
    63841 ggtacgcacc aatacggtaa cattacccgg cagcgattcg gccgtggtac gcatcaaagg
    63901 caccaacaaa gcgctcgcga tgaatgtaga ttgtaatgga cggtatgttt acctcaatcc
    63961 gcgtcgcggc ggccatatcg ctgtggccga atcggcacgt aacgtagttt gctccggtgg
    64021 acgacctgcc gctattacca attgcctcaa ttttggtaac ccgtacaaac ctgaaaatta
    64081 ctggcaattc aaagaagccg tcgaaggcat
//
LOCUS       DAOQGF010000005        62092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_152_length_62092_cov_7.098699, whole genome shotgun sequence.
ACCESSION   DAOQGF010000005 DAOQGF010000000
VERSION     DAOQGF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 62092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_62092_cov_7.098699"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PK937_01850"
     CDS             complement(<1..291)
                     /locus_tag="PK937_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH31346.1"
                     /translation="MNNLQFDFKVDKSTKTVFVEKEFDAELSLVWDAFTKKEILDQWW
                     APKPYASKTKYMNFKVGGRRFYAMVSPEGQENWHIQDYTSISPKTNFKFFSVF"
     gene            complement(288..608)
                     /locus_tag="PK937_01855"
     CDS             complement(288..608)
                     /locus_tag="PK937_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH31347.1"
                     /translation="MRRDVFQAIADPTRRAIIGLIALQAMTPNAIAENFNSTRQAVSK
                     HLRILTECELVKQEYKGREIFYSLEIEKMKEIDDWLEQYRKIWESRFSQLDNLLSTMK
                     RKRK"
     gene            complement(605..1102)
                     /locus_tag="PK937_01860"
     CDS             complement(605..1102)
                     /locus_tag="PK937_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH31348.1"
                     /translation="MSNPVNVIKNLKEKFILVTKEFNAPLPNVWRAFSEKELLDQWWG
                     PSPWRAETKTMLFKPGGYWLYAMVGPDSQKHWGRMNYIAIDLHHFIDMEDFFCDENGN
                     LNGELPISKGRIAFTKTESGTKVEFKTSYASETDIQKLVEMGFEQGITLCFEQLTQLL
                     NKKNL"
     gene            complement(1137..1514)
                     /locus_tag="PK937_01865"
     CDS             complement(1137..1514)
                     /locus_tag="PK937_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNH31349.1"
                     /translation="MRSKVFFAVCLLFGLMFINAGLNKFFNYIPVPPDLPEKMVKQSA
                     AFLEIGWLLPLVATVEIIGGILFIISKFRPLGAIIIFPVMIGIVMTHITVAPSGLPIA
                     LLLLAINLFIIYDNREKYIAMIK"
     gene            1821..5267
                     /locus_tag="PK937_01870"
     CDS             1821..5267
                     /locus_tag="PK937_01870"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013187760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HNH31350.1"
                     /translation="MIRSIKKLLVANRGEIAIRIFRACSEIGIETIAVYTYEDRYSLH
                     RYKADEAYQIGADDDPLKPYLNIDEIIHLAKKIGADAIHPGYGFLSENDQFVKACEDH
                     GIIFVGPNSEIMYKLGNKIAAKVVARENNIPIIESNREALKDTSVAEREAQRIGYPVM
                     IKAAAGGGGRGMRVVRNADELRARFDEARGEARNAFGDDTVFIEKFIDDPKHLEVQIV
                     ADHFGNVVHLFERDCSVQRRFQKVVEIAPALTVSRDARERLYHYAVQIATAVGYQNIC
                     TVEFLLDKEENIYFIEVNPRIQVEHTVTEMITGVDLVKTQILIAQGYPLSDARLKLDD
                     QSRIYYNGYAIQCRITTEDPSNEFKPDYGTILAYRSAAGFGIRLDGGSIGPGIRISPF
                     FDSMLVKVSAWGLSFEETCDRMYRTLIEFRIRGVRTNIPFLENVVRHPIFREGRATIN
                     FIKDHPELLQFVKRRDRATRILRYIADVVVNGNPDVKIRDDKKVFIKPRIPAYDRYGD
                     FPDGAKQKLDTLGPEKFARWVMDQPKVMFTDCTMRDAHQSLLATRMRTYDIMRIAESW
                     AKHHSNMFSMEVWGGATFDVALRFLHEDPWDRLRQMRRAMPNTLLQMLIRGANAVGYT
                     AYPDNLIERFIIEAAREGIDVFRIFDSLNWTEGMKLSIETVRNKTGALAEACICYSGD
                     ILDPKRSKYSLTYYVELAKELERMGAHILAIKDMAGLLKPYAADVLIRALKDNIGIPI
                     HLHTHDTSSVQAATYVKAIEAGVDVVDVALSALSGTTSQPNFNSIVEMLRHTEHQVDV
                     DMTSLNAFSNYWEAVRDIYYPFETDLKAGTAEVYNHEIPGGQYSNMRPQAISLGLGEK
                     WEEIKQTYHDVNMLFGDIIKVTPSSKVVGDLAQYLVANNMTAADVLMKGETMSFPESV
                     EGFFMGHLGQPYGGFPEKLQKVIIKNKTPLTDRPGKHLPPVDFDKEFEAFKQQFDANL
                     SITDFLSYKFYPKVFTDYFENQQKWGQVSVIPSEIFFFGMKPNEEAIIEIAQGKSIIV
                     RLLSVSQADKDGNRKVFFELNGQARTITVQDRSLNVKRVQNRKADASKPGELASPLPG
                     MLSKVLVKPGDTIKKSQPLCIIEAMKMETTVTAVQDGVVKEIILGVKTIVESGDLLMI
                     LG"
     gene            complement(5297..6013)
                     /locus_tag="PK937_01875"
     CDS             complement(5297..6013)
                     /locus_tag="PK937_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH31351.1"
                     /translation="MKPTVILIVDDNPHISKLLTMNLEQSAFTILHAMDGEEGLAKIK
                     TDKPDIVISDVMMPKLDGITMCQQVRTGSDVPMVPFIFLTSMDSDITQKRGFRMGADQ
                     YLIKSEINRDTINGKVNEILKHIRKIEEIRSQNSDTFKSDLGELSFIETLQYLYLQKK
                     TGTLTIIRQFYPEGKIIVRNGEILSATVGDDKDEKALLTISGWKRGTVQFLSDDVSAI
                     TPTVKTSTENIILESCRLTA"
     gene            6172..7164
                     /gene="moaA"
                     /locus_tag="PK937_01880"
     CDS             6172..7164
                     /gene="moaA"
                     /locus_tag="PK937_01880"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNH31352.1"
                     /translation="MPLIDGFGRSIDYLRVSVTDRCNFRCVYCMPHDGVQFMPREELL
                     TYDELERIIGVFVNLGIEKIRITGGEPTVRKDIIELVRRIGSLPLRSYAMTTNGYLLD
                     ELAEPLRAAGLQRFNISLDTMRRDRFQKIARFDGYEKTWRGIRKAADLGFAPIKLNMV
                     VLRGINDDEIFDFVELARERSWHVRFIEYMPSSGDLGNTFDASHIIRTDELKRIVSQK
                     YALIPITEAVQSGPAKMHTIEGFAGKIGFIDPYAEHFCAACNRIRLTALGKLKWCLFS
                     NEGLDIKSFIRSGKSDAELEAWIRERIVRDKPEHHPIAISDMIRANTVFSQVGG"
     gene            7249..>8788
                     /locus_tag="PK937_01885"
     CDS             7249..>8788
                     /locus_tag="PK937_01885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNH31353.1"
                     /translation="MKKPSILIIGGGINGAGVARDAALRGYHVGLFEKNDFASGTSWT
                     SSKLIHGGLRYLEHGEIGLVRESLREREILLHQHPHLVRPIRLTVPIYRHGKYGRLAM
                     RAGMWAYDLLSFDKSLPRFSLYTHRAVRARYPGINAQSLLGGVSYFDAQAIYPERLVL
                     DNIHSAVRYGAEVYNHTEVTGFLKSKERITGVQTRNTLTGEEAEYTADMVINTSGPWV
                     DHILKHTEKNIPPQLGGTKGSHIVIRNTIALQDALYVNARRDGRPFFIIPWLDYILIG
                     TTDLRYEGHPDTVFPESHEIDYLIEEATHVMPELISGRDDIVFSFCGIRPLPRDERRH
                     TGAITRRHGILDHQREGAEGLISLIGGKLTTYRSAAEQMVDHIEKSLGIAMQPCRTAT
                     EPMHPLMHHDTELLPLTAMLGSDAGAAAQMAQQKNWSLSPVAPDYQTSEAQIRYAVQE
                     EWARTLSDVMLRRTRLAYSPDNGLPVAHGVASIMGSALAWPQERTTAEIQAYTAYVNA
                     YLQAH"
     assembly_gap    8789..8888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9073..11148
                     /locus_tag="PK937_01890"
     CDS             9073..11148
                     /locus_tag="PK937_01890"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNH31354.1"
                     /translation="MEINTPVWAGTDEALNLAEKARHAIRASRGKLSAYKLTTYSKSF
                     IDLYKRKKNAFEMKSASLVHAEYYWRQPDEVREIEIARRHIDDFFYTDYNIEHLPIID
                     DLTRARITVGRASVAGPLTEDAAVYYDFSLAGDTVIGGVAVHQLRCKPKTEYEPLMDG
                     VVYIAQETFQVVGFEIQFNKAVKFFPPPDLLQVRVMYAETPSGVWLPKHIQWGMQFNI
                     TFPWPAQAQWRSETMVYETAINPEIPSAVYRGKAVETREDAYFRDSAFWADNEKLPMT
                     EDEAQGFSNLGQLPWNLKILNPDLKGYRKRQRQKDIDWGFQLIPDIRYNRVEGFFTGA
                     KIEFDDLAYKRIVRNLTIKTKVGYGFADEAVKYSVEAQKKWLDKRFYVGGKYYDDLAY
                     KEFTNDGSVLNNSLTSLVYRYDIYNYFYVRGYQTYTGVRPFRGARVEFAYTDRWDSSA
                     TKNAHYALIKSMYKDFDPVYPINDGRLRRITVSANYTIGTGTGIAPRTPYWIFDASID
                     HANPNFLKSDFHFTLYNFSARFHIPTTRRGSLDGKAALGLGTDRLPNQYLFHLYGGTT
                     PYVLKTVDFREPTPNHFQGNRMAALTMEHNFGGTLLERTDLPFLRDGYVDCIPTFSIG
                     FVHASKKTLENLRQNKIQYLRRPLVEAGFAVGDIYRIVRLDFTWRLTQRSKGNRNFAA
                     TMAVLVQRF"
     gene            11258..12061
                     /locus_tag="PK937_01895"
     CDS             11258..12061
                     /locus_tag="PK937_01895"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNH31355.1"
                     /translation="MEKRLAGQKALVTGANSGIGAGIVQRLAAEGADVMINWFANEDA
                     TKALVDKINKAGAGKAIAFRADVSNEDQVNAMYEAMFKEYGTIDILVNNAGIEIHNFL
                     ENVTKAEWDKVIGVNLTGNFLCSRSAIRQFLKNENRPNSRSRGKIVNISSVHQVIMWA
                     GFSSYCATKAGINLFAQSVAQEYAEKKIRINQVAPGAIKTPINQNVWSDPKGMADLMT
                     KMPYGRMGEVDDVAAAVAFLCSDEADYITGTTLYVDGGMCLYPEFRFGG"
     gene            12076..12675
                     /locus_tag="PK937_01900"
     CDS             12076..12675
                     /locus_tag="PK937_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31356.1"
                     /translation="MEDSKETSRNNPSETPETTRKYEREISDYIHFQIEETQAPESAF
                     EQKVVPESIREQGFWRRFHVRRRIFVSRTADFFKDTKKMVVVWIIIMALVAIGSYFGW
                     DREMVGGAVVFFGLISSAFSWLAAVILGGIGLIPFIGPVLVAILSSSLLWIINGLGYF
                     VSVVAIRAGHGKAVLNYRLLVIIFLCGIVTGYVIAKIVH"
     gene            12780..13535
                     /locus_tag="PK937_01905"
     CDS             12780..13535
                     /locus_tag="PK937_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH31357.1"
                     /translation="MSKSIDELLKDLQSNKVEVKEEAAFELGGYDDAKVVSALMGVLK
                     SQHEEVGVRAAAAESLGRLGKVEAFDALMECLKSGEYMVRSAAAYALGKLGDKRAADA
                     LFFLLKDSDHSVVSSAAHALAKLHDRRAVLPLIHIVETKRPRQQVAAVRVLGDLHDAQ
                     AVAPLVKLYKKISDPDLKHAIIRALDDISAPGCEDVLMEALDDSVSFVRRYAADALGN
                     IKHKPAKDKLMQLAQSDTDGDVRNVAKQAADKL"
     gene            complement(13593..14588)
                     /locus_tag="PK937_01910"
     CDS             complement(13593..14588)
                     /locus_tag="PK937_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31358.1"
                     /translation="MPSLLSVRHLSAHFHVENQLYKAVDDVSFDIAPEETLCLVGESG
                     SGKSITALSVLDLLPYPGTIAGGAIYWQDKKIYDSGIDHRKSLRGREVAMIFQEPLTA
                     LNPVLTCGEQIAEVLRLQPKTDSRTIRHQCLDLLERVGLNESEKVYSGYPHTLSGGMR
                     QRVMIAIALACKPKLLIADEPTTALDVTVQAQILDVLRDQQKRDGMALLLITHNFGIV
                     AGMADRVAVMYASKLVESGPVRSIFKKPAHPYTSGLLRAIPSLISDEKKLYGIPGQVP
                     RANQYPAGCHFHPRCMHADHMCRSTKPELIHVDDAHQASCFHPHAETSSTATDKI"
     gene            14683..15162
                     /locus_tag="PK937_01915"
     CDS             14683..15162
                     /locus_tag="PK937_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNH31359.1"
                     /translation="MTEVNTSPISLADLILPATIRLDLQGKTKQSVIDELIELISEAG
                     LVRDKSAVRQAVIDRERKLSTGLGHGIAIPHGKTSGVDKLVGAFGIKRDGIHFDSADG
                     APAKIFFLLVSPVTVTGPHIKALSGIARFLRIEKNRERLLQAATTDEVLALLGKPAV"
     gene            15162..16427
                     /locus_tag="PK937_01920"
     CDS             15162..16427
                     /locus_tag="PK937_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNH31360.1"
                     /translation="MQKLIIAAACFICILTPELTAQKLLIPMDLRQSDHLKAYGVAYT
                     ALENKVPVEWLLNYRGGSFMIAYTETMANYCLARGVKTENLSAAQVAVVYATIENENM
                     EIVYLEKAPHIAVYTPPDKKPWDDAVTMALEYAEIKYDKIFDLDVLQGKLAEYDWLHL
                     HHEDFTGQYGKFYSAYGRAAWYQQQQRDYETFAQQMGYSKVSKEKLGVVQAMKEFIAK
                     GGFMFAMCGATDSFDVALAAQKTDICDVVYDGDPVDMRANEKLDYGHCLAFQNFRVEL
                     DPYQYEFSSIDHPSSNGLRLKTPEEDYFTLFDFSAKYDPVPTMLTQNHVNVVKGFMGQ
                     TTGYRRELLKENIVVMGEDASIGSVKYLHGNFGKGTFTFYGGHDPEDYTHAVGDPPTD
                     LALHKNSPGYRLILNNILFPAAKKKPQKT"
     gene            16424..17785
                     /locus_tag="PK937_01925"
     CDS             16424..17785
                     /locus_tag="PK937_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH31361.1"
                     /translation="MKSSSPKIIIIGGVAAGPAAAAKAKRINPRAEVVLFEQGEHISY
                     GSCAMPYFIGDVIPEAEKLIHFTPASFEKEKGCAVQIFHRAEAIWPHRRRVVMRNLQT
                     DQIAEYTYDALIIATGARARVPDPAWFRYDNVFAVRSLSDSMRIKNYLASRRPSTAVI
                     IGGGFIGMEMAEALRRHAMHVTVLHKSEWPMDSLELSGRRVVADELKRQGVKFEGNIT
                     SPELVAGGQTVRAVISGSARFDADVVIIATGFEPNTDLAREAKIRCGASGGIIVDHHL
                     KTNADRIWAAGACTEFKNALINKPMYLPLANIANKMGWIAGENAAGGFAEFPPVVRNT
                     AVKIFDLEVASVGLDAASASAHGLSVIEETIMAHSRVRAYPGAKPVMITLLADKHSKR
                     LIGANLIAEEGAALRANILALAIQQKMTLRQIADMQMMYTPPFAPVWDPILVAANKMM
                     KGL"
     gene            17782..18237
                     /locus_tag="PK937_01930"
     CDS             17782..18237
                     /locus_tag="PK937_01930"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNH31362.1"
                     /translation="MMNPHASDESSITVKQIETKPLSRPMRILFVIAGHIFVALGVIG
                     AILPLMPTTIFLILAGWCYARSSERFHCWLYTNKFFGTYLRNYKENRGTPLSVKIGSI
                     SVLWISIGISIWTTSMPWFVPWILIVIASAVTVHLARLKTLKKESSHES"
     gene            18227..18760
                     /locus_tag="PK937_01935"
     CDS             18227..18760
                     /locus_tag="PK937_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019264126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNH31363.1"
                     /translation="MSLKDLPIGKKSPDEVHAIVEIPSGSRNKYEYDVELEIFKLDRT
                     LYSAVHYPTAYGFIPSTFYDDGDPLDILIVASQPLHVGVLVEARPVGVLRMRDDKGPD
                     DKILGVAIRDEQFRDIHRVEQLPHHLLVEIEHFFTTYKHLEGKDVRSFGWEPEAFAKS
                     AIMRGHQKYLERKSSHA"
     gene            18753..19175
                     /locus_tag="PK937_01940"
     CDS             18753..19175
                     /locus_tag="PK937_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH31364.1"
                     /translation="MPERTHRMAVTAYIVHDGKFLLLKRTNPPMIWGPPGGRLNPDEN
                     PNEGVLREVFEECGLTVKILHPVDIWYGDFGRGIYVSVDYLAISPSSEVSLSDEHSDY
                     IWSDMNALRNGQPSLGSAAPAFTIHDFRRAWELYQKLL"
     gene            19204..20757
                     /gene="guaA"
                     /locus_tag="PK937_01945"
     CDS             19204..20757
                     /gene="guaA"
                     /locus_tag="PK937_01945"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNH31365.1"
                     /translation="MSHGETILILDFGSQFTQLIARRVRELQVYCEIHPYNMTPDKIR
                     ALQPKGIILSGGPSSVYENGAPHPDRAIFDLGIPVLGVCYGMQVMAYFLGGAVDKAAH
                     REFGRAEIRITDHTDLFSGFPEKTTVWQSHGDRLEKIPDGFEIIAGTDNAPITAMRNK
                     ARRFWGVQFHPEVAHTPLGINLLKNFVRTICACTGQWTPSSFVKETTDAIRAKVGDGY
                     VICALSGGVDSSVVAMLLHQAIPDRFSAIFVDNGLLRKNERGQVEAAFRSFFNNNLIV
                     ADASELFLTRLKDVTDPEKKRKIIGATFIDVFETEVEKLRATTGHIDFLAQGTLYPDV
                     IESVSIKGPSATIKTHHNVGGLPEKMKLQLIEPLRELFKDEVRAVGRQLGLPDDLLMR
                     HPFPGPGLAVRVLGEVTKERCDLLREADAIFMEELKKSGEYSNVWQAFTVLLPVKSVG
                     VMGDERTYQNAVAIRAVTSQDGMTADWAHLPYDVLGRISNRIINEVRGINRVTYDISS
                     KPPATIEWE"
     gene            20764..23964
                     /locus_tag="PK937_01950"
     CDS             20764..23964
                     /locus_tag="PK937_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31366.1"
                     /translation="MKLLRWHNLVFLIGMMHPIALCVYSQNERLYFDLVDLPKTMSPA
                     VRSIVQDHQGLIWVGTEKGLGRYDGYSWRVFHSDATDSFLISDANVWTVFEDRKGYLW
                     IGTINGGVNVLDRNRSRFHHYKHQPNNTASLSNNTVYSILEDNKGVMWIGTENGLNCI
                     YPESNKVMRLPDQTTDPSGYKGKIIHSLAMDSLYRIWIGSDSGLTVYDPQTCQFSVFQ
                     VQEHGRIYTPRRVRKLYADEDRQTLWIGTFGGGLYRWGIHTHEIKQYDVKSVENAYPS
                     NYVRNLVTDRDHEHLWIAIRGGGLGRLHKASGQIRYFTRQPNIPLSIPDNASLALYRD
                     DLGSIWVGFNYDGLAIMNYRKSWFQHIRRDATGTLVDHTVRSVCVAGDGTTLFGVQRA
                     VHRYDPHTDRLQSFVMPEHVVNGFNQVQAILYDKGQNYWIGTYGNGLNRLDAATGKFS
                     KYDAYPSGVQQRILSLTRFGDFLWIGTPSEIIRIHPATSSSRIYKNIRAVNSFAQTNT
                     QKLIAGSGTNGLFFYDAVADSFMPWLSSSMKYPSDYETRSLFADSQYIVWQGTSNGLN
                     KINHKTGEITRYTRNDGIPSTDVTGIIQSPDQRLWISTTSGILRLDPGQSQFRYYYKE
                     NGLLGNEFSLSAVAMSSEGRIFFGGIQGVTWFNASDVRDYDYKPTLLITKISRFYQDV
                     DGEIPYNSIEAQYNENVLTFEYSVLDYAFSAGIHYAYRLEGFNQTWIQTDERHATFTN
                     LDPGEYVFRVRGTRGDGTWIDSESKVFITIHPPYWLTWWFWSLVVFCIGMVTWMGYRM
                     RVRSLLRRKRELQTEVERQTKELYAKNIELQSLNDTKNEFLGMAAHDLRNPLGAVGNI
                     AELMLFEMNPENPETKQTFSDLNRIIQLTERMSYLIHELLDYSAIESGKVSLAVDTID
                     WTVLVHEVCEMNERAASRKKIVIKKEFSDGTIVLHGDRMRLMSVLDNLVSNAIKYSFA
                     GTEVLIRSEVANRKLRVTVYDHGQGLPEADVARAFKSFQKLSAKPTGGESSTGLGLAI
                     IKKIIDLHAGQVWLESELHKGSTFGFSLPLERPFSD"
     gene            24339..24878
                     /locus_tag="PK937_01955"
     CDS             24339..24878
                     /locus_tag="PK937_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNH31367.1"
                     /translation="MHGIVFIELRKFVEVGFGRDGWMQILKKADIGEDLFMPSKTYPD
                     HQIVSIVLAACEITGKSAHEILDAFGKFIVPDLIGIYGSFIDPKWTMIDMIENVETTI
                     HRIVRMKNAGADPPELSCTRLGKNKIEIAYKSKRKMCAFAKGLVAGLANHYNVKATVT
                     ETLCMHNGDAECRMQVVTS"
     gene            25292..26404
                     /locus_tag="PK937_01960"
     CDS             25292..26404
                     /locus_tag="PK937_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31368.1"
                     /translation="MRKLGVILLLLQTRLIMAQATVSDAQILADVRRSRPDAATVKVV
                     GSSWSFYKDKGYLYNNRAEKTVDLTTTKNSDGVSFSMRGAAVYESSGPGQPYMYSRLF
                     VFESSVTMIGGPNYSVTDLQKIAYETARRDILRWFSNPQSIARVYEIKVPENHDLRMS
                     ALDHMSFRVQQTVLRVDNTNTVHKEVHEDVLTFRKNKDGWFVTSPLTYAKKKTSLGSM
                     KPGTRVLSSVPSLQDVGLDRLYGDSKDDFFKDGVDQGEKSPTAKDIFSRNKSQNDPSG
                     GITIPKKKDDGESVTDLPEDTQVRNDIMSRDADVAAVSFIGNWNTRKTKEGGGVNLAG
                     RMVVLKKKNQNSDKFVVVYNKDNGLWKLKAVEKSPQ"
     gene            complement(26425..27123)
                     /locus_tag="PK937_01965"
     CDS             complement(26425..27123)
                     /locus_tag="PK937_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH31369.1"
                     /translation="MAFLPFRILVIEDDPDISRNINKLLEHAGYVCATCDEGSVGFEK
                     ACSGMWDAVILDIHLPVKDGLDIVRDIRRANINVPVLMVTGRNSVPDKVAGLKLGADD
                     YLTKPFHNDELLARLDALLRRFGRAQTVSPSEVYKHAGLFADFKSGEFKLENKPFKLT
                     AHEMKMLHYLVQHQGLIITRDELLTHVWKYGDTVTTRTVDVHVAAIRQKIEKIPSKPK
                     RLISIYGLGYKFIG"
     gene            27256..28491
                     /locus_tag="PK937_01970"
     CDS             27256..28491
                     /locus_tag="PK937_01970"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH31370.1"
                     /translation="MTTFSDILLNLRRQSKSKPATLPKNECTTYRGSVCTICPLIQSN
                     YNDELRIKNEAIREFWRMHRLDGKPESIIASPLSRRYRTNSKRRAFITDNRVLLGLTG
                     MDDEGKIKPLDVRQCGIEPLLHSRIYAYVQQWLSSEASYMFARALNYVILRGDDRRIT
                     ILLSVRDEDNRIKRDCTLLSKSLSREFPMVVGFLLIRDEYSKYYMSDKAVSDPTALQK
                     IFGERDVVQDVGEQQFSHSVLGFSQTNPSVLPDFIAMVHDLISPQPHHTLYDLYCGYG
                     LFGLSLAPNVEKVIGVEMSRHSVDSARTNALRMKIANARFHIGDLSGLTLEPILKKQK
                     TPFIAVLDPPRSGTAPGVIETLAMAKPDRVVHVFCDIERLPYELKRWRQSGYRAAHVI
                     PVDMFPATGNVEVAVLLET"
     gene            28568..28639
                     /locus_tag="PK937_01975"
     tRNA            28568..28639
                     /locus_tag="PK937_01975"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28601..28603,aa:Glu,seq:ttc)
     gene            complement(28814..29608)
                     /locus_tag="PK937_01980"
     CDS             complement(28814..29608)
                     /locus_tag="PK937_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNH31371.1"
                     /translation="MKSDNRYKQIIEKIFFNHFKNGMKEVPFERDEIERVAKNLKIKL
                     PKNIGDIIYTFRYRVALPDSIASKAPKGMEWIIRPAGQSKYKFVIAKFANIHPSEILS
                     QTKIPNATPGIIESYAFSDEQALLAKIRYNRLIDIFTGIACYSLQSHLRSNVSDLGQI
                     ETDEIYVGIDKKGVHYIIPVQAKGSADKLGIVQIEQDIALCKSKFPNLVAKPIGAQFI
                     TDDLIALFEFEISENGITVVSEKHYRLVNPENLSPHELLSYRNRTE"
     gene            complement(29611..30963)
                     /locus_tag="PK937_01985"
     CDS             complement(29611..30963)
                     /locus_tag="PK937_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNH31372.1"
                     /translation="MPSNQFAYNSFVEWDNQINGKRSPFSPLNNKLNLDDKRFHDWYR
                     FVLSYPAHLVRTYLQEFCSSQNDIVLDPFCGTGTTLVEAKLNGINSVGIEANPFAHFA
                     STTKINWEISADQFLKCALEIADLVRSEFEKSGIDDDDINMPEHTFPLRTLDSELEKI
                     ILTNSISPIPLHKTLVLLEKIKNHAQKKYYPHLLLGLAKATVFKTSNLKFGPEVGLGK
                     IKKNVPVIASWLFEINAIANDIRSIQIGKPKESVAHLGDSRQASALIKPQSITAVITS
                     PPYPNEKDYTRTTRLESVLLGFIRSKEELRQFKKTLIRSNTRGVYKDDDDHQWIANHP
                     EIKRIADQIEARRIELNKTSGFERLYSRVTLLYFGGMAKHLKELQPVLKPGARLAYVV
                     GDQASYLRIMIRTGEILADIARKLGYRVDRIDLFRTRFATATGEQLREEVVVLTWPGN
                     "
     gene            complement(31259..>32137)
                     /locus_tag="PK937_01990"
     CDS             complement(31259..>32137)
                     /locus_tag="PK937_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31373.1"
                     /translation="GTYWISYTTETGLPSNDVKAVLFTKDETVWVGTDKGIHIRKAEG
                     DGDRVLLIRKVLAEEDSLLNIRAYDKLRTIYASVNIFGDLTEWVAYKTANTYEMEKRW
                     DDAFQLYTKIRTDNPVTTLATDARMYRVARAFENAGESAKAMPIYFDLAERYKTYTDG
                     NYRVEAALVRIIRNYQKENNIQRALEQARQIMAMYPRSPWLREVRFIFTQITENAPPK
                     ETSTMALLQEWLNRFPDHSDVLRLKYKLASMYWEEQQFEYAEKLYAEINSAAGTPKGL
                     QSLLERRMAKRQLTKK"
     assembly_gap    32138..32237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32238..34294)
                     /locus_tag="PK937_01995"
     CDS             complement(<32238..34294)
                     /locus_tag="PK937_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31374.1"
                     /translation="MKRIIALVLSLCAAVSASAQWPRLSVKTAVTAIAVEDTVTWIGT
                     NDGIRGVVNGTEKFYFDKGNGLTSNIIRAITIDNQNRKWIATEEGLNVLDGKKVKQYL
                     KSPETLPTNEINSVHADEEGSVWIGTEKGLVRISGNSWTLLDRKTTLDGLPSNTIRGI
                     SSVWNGPVWIATDDGLVSYYNKKWALYTRKLNNLPSDDVFDVVADPSSGNVWIGTSRG
                     VALFDGKLMTQFLSDIPVRALTLDKIGRCWVATKNEGVHLIDGARVVKYSTKEGMLVS
                     DEGYAVTCDAFNTIWAGHQLGVQTFLDETIGSRIAPYFLTEAQKAFWSNDNTRARLYY
                     NHIVNRSYLSATPEAATAMIGLGELYEREAMIERAADTYLRFTERFPNDARTKNLLLK
                     SAQLQSQIKKYAESAATYQKFLTLYKDDADAFTIAWMRYNLLELAGDSVSALQTLQNL
                     NTQFPSNPRADEIRWRILSAGNSAGVASKELRALANSTRDFEVQYQLAALYDTQHRKD
                     ILEGLQGSITWQTLFSPSAVTSIFEDGDIAWASTSGNGVVKWDIKLNALTSYSDGLKT
                     PTVKSFYMDADRDLWAMIEGVPESNLYNMNYSKNRYKWIIQPAPFAKKTINQVLYRAK
                     TKTTICATDQGLQIVGKGGRLVTAKNGLPSDKVKFIADDSQGILWLISDNKLVQFDKE
                     PKM"
     gene            34413..35582
                     /gene="aroC"
                     /locus_tag="PK937_02000"
     CDS             34413..35582
                     /gene="aroC"
                     /locus_tag="PK937_02000"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNH31375.1"
                     /translation="MIRWLTSGESHGSGLVGIIDGVPAHLKLSVDVINHQLWRRQQGY
                     GRGGRMKIETDQAEIISGVRFGETIGAPIGLRIRNNDWENWTDKMMTETPSPEILKTV
                     KPVTLPRPGHADLAGIQKFGFDDIRPVIERSSARETAMRVALGTVAREVLLSCGIRIA
                     SHVTHIGRASLTAAESEKLRVDTMTTEAINQKADLSSVRVLDAEAEQRMIAVIKETQK
                     QGDSLGGIFEVYADGLPAGIGSYTHWDKKLDGVIAQHMASIPAIKGVEIGNAFDNALQ
                     WGSEVHDAIFYDDVRGYYRKTNRSGGLEGGMTTGERIIVRCAMKPIPTLARPLMSVDI
                     RTHEAASAHKERTDTVSVPACSVVAESMLALALINPLLEKYGGDSMNDIRTAMGR"
     gene            35777..36673
                     /gene="xerC"
                     /locus_tag="PK937_02005"
     CDS             35777..36673
                     /gene="xerC"
                     /locus_tag="PK937_02005"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNH31376.1"
                     /translation="MIRQHLHDFLKYLDAERNFSDKTVGAYHTDVAAFISFVETRFDS
                     SSFDASDISKNEIRAYLGQLSRDRLQKKSIARKLSAVKSFFKYLTKQQIITANPAKLI
                     ATPKFERKMPAFLGQEQVRDVFDYVDAGTPEGLRDRAILELFYGCGIRLSELTGLNFS
                     DINFTGGTLSVFGKGAKQRIIPMGKHAGAALQSYIDYRRSVSSADKNAVFLGKNGKRI
                     TPLAVQRLVKRALEKVSDAKKLSPHILRHSFATHLLDNGADLRAVKDLLGHENLSTTQ
                     VYTHVTVDRLKKVYEKAHPRAK"
     gene            36767..37102
                     /gene="raiA"
                     /locus_tag="PK937_02010"
     CDS             36767..37102
                     /gene="raiA"
                     /locus_tag="PK937_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNH31377.1"
                     /translation="MKIRITARHQKMSPKVKAYIEEKLEKLDRYHDHIIDCEVIVDKE
                     KLKEVVEVNVKVFGKVINVKAKDADVTKATDLAMDKLESQIKKFNDKIKDKKKKSVKK
                     MPIIDEDEE"
     gene            37215..38819
                     /locus_tag="PK937_02015"
     CDS             37215..38819
                     /locus_tag="PK937_02015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH31378.1"
                     /translation="MANLNQSYDFIKQIGVGGMATVFLGRHPALERLVAIKVVKGEQK
                     DKVRRFEREARLSATLKQENLPAIFDYFTDDQNNHYLVMEYVDGVDISPIIKSKTTIP
                     PMIVAMIAREVCRGLEHMHENGIIHRDIKPSNVRLGKNGQVKLMDFGIAKHEEDAGKS
                     HLTATGVIVGTPSYMSPEQASGDQLTPQSDVYSLGIMMYEMLTGKKPYTADTNMTLIS
                     LIAQGKFTPLSETHPHLPPALVDIVHRAMVKSLRQRYATTSEIIKDLNKYLQSMSQIE
                     IRDWLVRYYEAATTKDKIVDMNVFVLPAPVSVETAETSTRPVDTEKNNNFVLNKRHLV
                     LGSAALVVLCVLAYLFWPSDHPLGNYDPYGRLALSVRSDRQTIKDTRVYVNDSEFPLD
                     DNFGGSLIIDNLQPGRNAIKIRFPLLYHTYEYNFTLDGADESKALNLDLTKIAASLDG
                     VRPESRRYGFAVISEPAGVSVHLDDLKSKPLGKSPTGLIFPSVKTGLHKIFLQKEGYV
                     TSAIERNFTPDQNYTLQYELGPAKKK"
     gene            38816..40222
                     /gene="rlmD"
                     /locus_tag="PK937_02020"
     CDS             38816..40222
                     /gene="rlmD"
                     /locus_tag="PK937_02020"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNH31379.1"
                     /translation="MIETTDIPILRKGDDVTLDIETVTFGGQGVARYHDFVVFVEGAI
                     TGDRVQARIMKKKKNFAEARSLQIIEPSRFRVDHACTHYGICGGCKWQDVDYDAQLEF
                     KRRNVIDVFERIGGFKGLDIPAPLGSPRIFHYRNKMEFTFGDTEWIVDKTSETSPRSF
                     MLGMHVPQRFDKILHIDQCYLQSPLANDILAFVRDFAQRSGLPPYSVQTNEGFWRFLI
                     IRETVHTGHLMVNVMTYSDQPEVMKEFKQAIINRFPQITSLINGITDRKSQVAFAERE
                     NLLHGQSVITEKLDGLEFEISSSSFFQTNTLGAEVLYRTIREMADLKGHEVVLDLYCG
                     TGSIAIYIADRAREVIGIELIESATRNAEKNAQRNRADNCRFINGDMRTTLDTLQTKA
                     DVVILDPPRSGLHEDVIRSVMRLGPDKIVYVSCNPSTQARDLALMQADYTIERIQPVD
                     MFPHTYHIENIVQMRKKV"
     gene            40219..41670
                     /locus_tag="PK937_02025"
     CDS             40219..41670
                     /locus_tag="PK937_02025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH31380.1"
                     /translation="MTSKRHNILCVDDEPVILNILSKLFEEKYQVYTAKGALEALELL
                     QQHSVAVAIVDQKMPKITGVELLKRIKEQYPNIVRIVLTAYTDISDLVASINEGEIFR
                     YITKPWDSEALMWDVHAAIEKYEENLANAQRIDDITRLQLEKEKLQKEIILLKSEIEK
                     EYALDNIVSVSPSMNQIRRIIKAASLADETVLITGESGTGKELVARAIHHNSQRQNFP
                     MVAVDCGALSESLLESELFGHVKGAFTGAVADRKGLFEEADGGTIFLDEISNTSLPLQ
                     ARLLRVLQEREIRRIGDTKSRPVNVRVIAASNRDLVEECKTERFRSDLFYRLNVLPIA
                     LPPLRDRRDDIPLLINYFINEYNKKSAKSIVSISREAMDHLSSLEWKGNIRELKNIIH
                     RMMVFAEGSHLEMGDIPDELLKVKRSAVVPVLPEGSTVFGVSALLPIEDMEREYIRFV
                     LHQTGENKAEASKLLGMKRTTLVMRMKKLGLLA"
     gene            41674..42258
                     /locus_tag="PK937_02030"
     CDS             41674..42258
                     /locus_tag="PK937_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNH31381.1"
                     /translation="MTRFQYILFYKPYGVICQFSSHEKHPSLAEFKFPKNVYPVGRLD
                     TDSEGLLILTDDGDFQHRLIEPRYGHARTYHAQVEGIPDDGALDKMRHGILLDSKPTL
                     PARVTVLEPPPDHPLRNPPIRYRKNIPTTWVELVLTEGRNRQVRRMTAAAGFPTLRLL
                     RVGIMSLSLEGLQPGSWRAMTTEEFRSCVAVVKK"
     gene            42314..42733
                     /locus_tag="PK937_02035"
     CDS             42314..42733
                     /locus_tag="PK937_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31382.1"
                     /translation="MHFSQSELLINILFLLFIIVLAIQSYILYRVRRSLTQASQFIQL
                     MNVFFKEISKTHSKSASLAVNNQDVMRKLRSKTIRSNPSHDVSCQACKYRLSYVSLDR
                     PEMDFTYACQLTRATVVLDDQCEKFEPDAESAQPSNK"
     gene            complement(42799..43665)
                     /locus_tag="PK937_02040"
     CDS             complement(42799..43665)
                     /locus_tag="PK937_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNH31383.1"
                     /translation="MIRPRNVTLRKRKQLLDNLYNTFDASYIASDPIQMIHCFEQPCD
                     QEIMAVLASAMAFGQVAQINKALSSMLTLMNNQPHYYVTHFDPEHELPRWQKWYYRMI
                     RAEDVLRVLHALKIILQKHQTLGKWIESHYRNEDVHLGITWSRCVNELKDTDKKHWQW
                     RHARGVGFTFLLADPSKKSACKRAFLMLRWMVRKDGIDLGLWNLPTSKLLIPVDTHIQ
                     RIAFYIGLTKRTDTSQKTAMEITYALKKLDAIDPVKYDFAICRLGILKMCPRKRRIVL
                     CRACPIYDICLL"
     gene            43938..47987
                     /locus_tag="PK937_02045"
     CDS             43938..47987
                     /locus_tag="PK937_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31384.1"
                     /translation="MIKKRILVAWLFFIGTTFLFSQSREFEFKRLTVENGLSQSNVYA
                     IVQDYRGFIWFGTQDGLNRYDGYQFSVFKHDIDDSNSISMNWINTIYEDKHRVLWVGT
                     QGGGLSRMNQTTEHFRNFTADGQAGSLPSNDVYAILEDHIGNFMIATGRGIVYMDREK
                     ETFRPASEIPELKYGQIRALCVERSGTVWAGTQLGGLFVRDRQGQWFRISMQVAGRPQ
                     SDELAVSSITEDVTGQVWIGTDKGLFNWDNGTMSKYTSPKLENKVLTDGPINHVSTDR
                     AGRVWIGTDNFGLFGIFDTTRSEAVRFINDQLNPRSLSENRVYSTLEDDAGNVWIGTG
                     GGVSIWNARANKFRVYRRSPTSQNTLSHNHVWDILEDRMGFLWVGTNDGLNRLDRKTG
                     NWKNWRYNKTHASGLSAERVWTIYETRDGEIWLGMSGGGLSRYDAATDRFIHFLHDQK
                     NPASINNNRVRSIAEDHDGILWVATRGGGLNRLDRKKNVFTSYQYDGPDGKGISSNDT
                     YVLLVQDSVLWIGTFDNGLNSLNLRTMTFTRYTHDPRNPSSISSNKISSLCLDRNGAL
                     WIGTTEGLNRLNHQRNSFERYTPKDGLPNEVILGVLEDNKGFLWLSTNRGLARFDPLA
                     PRGQKFRNYNPNDGLQADEFNGSSFHKSKSGELFFGGVNGFNAFFPEEIKDNTHVPSV
                     VLTAFKIFDKPFRLQGAIAAMDQVTLGQKENAFSFEFAALDFTAPTKNQYAYKMEGFD
                     EDWIYCGTRRYAGYTNLDGGTYTFRVKASNNDGIWNNEGIAVQVTVVPVFYKTVWFRI
                     LASLVIVLVAYSFYRSRLNRVNRIKELLERKVAERTKDLSEMNAKIMETDRLKSEFLA
                     NMSHELRTPLNAIIGFSELLIDEMRHSANADQLQNLSDIHDSGRHLLMLINDILDLSK
                     IEAGKMELFPQQFIMEELLESVHHTMAPLLNRKNQTLSWQLDTNAPAVYADQNKIKQV
                     LINLLSNAIKFSPHDSAIIVTSKALNTDENDMVEIAVHDHGKGIAPENLDLIFEEFRQ
                     IDGSHARAEQGTGLGLALCRRLIEMHGGKISVESEIHHGSTFRFTIPRAVAGSSQEVM
                     SETPVDLTHQSLILMIEDDPQSANLLRRYLEIAGYRVAHVRNGSEALEEARRLKPMAI
                     TLDIMLPGKDGWQILQELKNDADTSDIPVFIVSVAENKELAYSLKAEDYFTKPIDRER
                     LIHRIGQLRMRRITGEIRQILIVDDDPNALSILESFLYKNGFRVLRAHGGREALVMMH
                     GATIDLVLLDLMMPGMSGFEVVEAMRNEEKLREIPIIILTAKDLTAEERERLRMHVRA
                     LMQKATYTTEDLIEEIRRIHPKTGALSNS"
     gene            47984..48364
                     /locus_tag="PK937_02050"
     CDS             47984..48364
                     /locus_tag="PK937_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH31385.1"
                     /translation="MIKQKTILLVEDNEVNMRLVERTIKNPAYTIVSAGNGEEALERA
                     AQYKPDLIILDIQIPKMDGYAVARTLKADPALKHIPIIALTAHAMKGDEEKILAAGCD
                     SYVSKPIEVSKFREKINDLLSDKS"
     gene            complement(48395..49363)
                     /gene="rfaD"
                     /locus_tag="PK937_02055"
     CDS             complement(48395..49363)
                     /gene="rfaD"
                     /locus_tag="PK937_02055"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNH31386.1"
                     /translation="MILLTGGAGFIGSAMLRKLNDLGHDKIIVVDNLGTSDKWKNLIG
                     KRFTDYIHKDEFLRLLDNKAFFDDTTAVIHMGACSATTELDADYLMRNNYHYSKRLAR
                     WALARNIRFIYASSAATYGGGELGFTDDDAQMSALKPLNKYGYSKHIFDLWILQERLQ
                     SRVAGLKFFNVFGPNEYHKGDMRSLVNKAFYQVLESGKIRLFKSHRPNYADGAQVRDF
                     VYIKDTVDIMAWLLEHRDVGGIFNLGTGKARSWNDLAKAVFHAMNKQVLIEYFEMPEI
                     IRNAYQYHTEATMTKLQEAGCPIRFRSLEESVKDYVQNYLSSNTYL"
     gene            complement(49634..50692)
                     /locus_tag="PK937_02060"
     CDS             complement(49634..50692)
                     /locus_tag="PK937_02060"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNH31387.1"
                     /translation="MKRIAVIPGDGIGTDVINEALKVIEAVQTRKPMDIQLTTMDYGA
                     EKYLKTKVTMPEEQYDDFRNNYHAILMGAFGDPRVPGNEHAKDILLGTRFKLDLYVNF
                     RPVKLLDERFCPLKNKTVHDVNFTVFRENTEGLYVSVGGIFKKNTPDEIAIQEEVNTR
                     KGVERIIRYAFEFAAKNGHKKVAMADKSNAMTYGHNLWYRCFFEVAKEYPQIQAKHFY
                     IDALAMMMIREPEMFEVIVTNNLFGDIITDIGAQLQGGMGLAASGNINPNGVSMFEPV
                     HGSAPDIAGKDIANPVASILTAEMMLRHLGFTAQADMIDRAVRVAIQKNKLTKELGGS
                     EGTKACGSFIAEVIRAEN"
     gene            50868..51569
                     /locus_tag="PK937_02065"
     CDS             50868..51569
                     /locus_tag="PK937_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH31388.1"
                     /translation="MSISLQEYFHAIAEATYGKIGDDFFKSLVRNLSSVLKMEFAMVT
                     ECANSEKSRLRTIAHWIGMDFGKNFEYDITHTPCHMVIHGGLQCYTEDLPAQFPLEKE
                     LSEIQATSYIGIPLKKSNGEIVGHLALLDTRPILDAEIKASILKIFASRAEAELERLQ
                     DKRERELLIYELQESLELVQTLRGMLPICASCKKIRDDKGYWNRIEEYVERHSGAQFT
                     HGICPECSVKLYPDL"
     gene            complement(51588..52511)
                     /locus_tag="PK937_02070"
     CDS             complement(51588..52511)
                     /locus_tag="PK937_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNH31389.1"
                     /translation="MKRGKFFLFTGLSILLLFICACQKKKEPIITDAAYVQEINAWHA
                     QREANLKKEKGWLNLVGLHWLKEGDNTFGSDSSNQLIFPPQAPPFAGTITLQKGGITL
                     TVKKDVVITADGKTISTSVLKDDSDSLMTRLAMGSFRFYVIKRNGEYAIRLKDLESPL
                     LKSFQGIDRYTVDKNWQTHATIKKYDPMKIILIPTILGTVDSMKCPGLLTFTIDDQTY
                     TLEPVLEDGSDDLFIIFGDRTNGSETYGGGRFLYTKMPAQGDSVIIDFNKAYNPPCVF
                     TDYATCPLPPLQNKLPIPIKAGEKMWGHKKH"
     gene            complement(52498..53634)
                     /locus_tag="PK937_02075"
     CDS             complement(52498..53634)
                     /locus_tag="PK937_02075"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNH31390.1"
                     /translation="MIKPGSTLGMLGGGQLGRMFTMAAHAMGYRVIVLDPDPQSPAGH
                     IADRHLTADYHEAEALHILRTECSAITTEFENVPADTLEILSHHCFIRPSAKAVSVTQ
                     DRLHEKSFLRIHQIPTNKFAAIRTESDIDEAVHFVGLPAILKRNRYGYDGKGQYRVRT
                     VQDVRNAFRAMHDAPCILEEMLNFEKEVSVVLARAVDGSITEYPTGENIHENGILDMT
                     IVPARIAGTMDSQARTIARQVAEKLDYHGVLAVEMFVMPDGRVLVNEMAPRPHNSAHY
                     TLDACITDQFEQQVRVLCGLPAGDPSLLSPVVMVNLLGDLWGNQQPHWEKLFTQTHVK
                     LHLYGKSEARPGRKMGHFNILDRNIENAIALGRQLKKDVAYETR"
     gene            complement(53679..54170)
                     /gene="purE"
                     /locus_tag="PK937_02080"
     CDS             complement(53679..54170)
                     /gene="purE"
                     /locus_tag="PK937_02080"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNH31391.1"
                     /translation="MAQKILIGVVMGSDSDWEVMKYAVEQLKTFKIPYEARVVSAHRT
                     PELLFSYAASAQERGLRCIIAGAGGAAHLPGMLASKTIVPVLGVPVPSRYLKGLDSLL
                     SIVQMPRGIPVATFAIGESGAANAALQAIAILATTDDTLAKKLSAFRESQTRKVLQLK
                     LPK"
     gene            54348..54812
                     /locus_tag="PK937_02085"
     CDS             54348..54812
                     /locus_tag="PK937_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNH31392.1"
                     /translation="MFKRVIETSDDSILTILRVVSGSVILMHGLQKLLGWFGGHGVTA
                     TMQAFEAWFGMPYIVTLLVILSDSLGAICLIAGAVTRFMAASILSVMIGAIILVHGRW
                     GFYMNWYSEKRGEGFEFHLLVIAIMIVLIMRGGGLWSVDRWLVKKFKATDNI"
     gene            complement(54885..55655)
                     /locus_tag="PK937_02090"
     CDS             complement(54885..55655)
                     /locus_tag="PK937_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31393.1"
                     /translation="MKKIILLFICAVSAAFAQESNPATPYQHRQLVNVPTAGMLNKGN
                     YEIGMRLFANGGVLSNIGVGITDRFMIGISYGGEKIIGSGKVNFNPLPGVDVRYRAVD
                     ESTIGPAVTLGFNNQGFGPYYKSVDTVKVNRYTQKSRGLFITASKNYAFAGNLGIHGG
                     ISWSVTEKKDKDNQPAFFVGVDKSINDELTAVWEYDLALNDNKDIIGHGRGYMNVGAK
                     LNFGQKVMVEFIMTDILNNSKDIGKFSRELRLSLIHAF"
     gene            complement(55724..56899)
                     /locus_tag="PK937_02095"
     CDS             complement(55724..56899)
                     /locus_tag="PK937_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31394.1"
                     /translation="MATSTAYAGGSSYSATTYGLYEYKPNPRSLGMGGAGLAIPSYYT
                     LNMQNPATLLGIPMTRFELNMFAEWNQASNGASKNFSNRATINHVAFAFPFGKTLVAA
                     INLSRVTNVNYEFSTPNLIFEGEKYSETLIGTGGLQQLAFTGAGKLNPKWTIGASLQY
                     LFGTIKREWDINYSSDELVDTRDVRHQNMNGGRLVVGGLYQHNEQWNFGGFLSMAGKI
                     SSDIRVVGTYRDTTVSYEQNLNYPTEIGFGASYRWTKRYLLATDIVYGTWGSVKEFSP
                     TQKNRNTVRWSSGIERQPSNAFSAPWWDKNYYRFGLMINNLYATNTTGNHASEIMFTL
                     GIGMPFNRERSSFDLSLEAGQRGQIADNKVRDRVYRLTLSFTGGERWFLNRQKRKRD"
     gene            complement(56954..58207)
                     /locus_tag="PK937_02100"
     CDS             complement(56954..58207)
                     /locus_tag="PK937_02100"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNH31395.1"
                     /translation="MEVIDRGTSTGTFKTDTLYADDIERIASFVDSISTYNSSRLYLG
                     SVDNYDFRILMRFAFPSDYITDPSGELINLDSLIIVKTRLQLVASDAFGSAGSINAHI
                     HTVQTPWSELDLLWHKFQQGTDYSPNSIGTINFSKLNDKDSLEITLNKDSVVQWVKAR
                     NDTSTINPKTNNGIIIDFDGGAPFTQQFYSGNNTIITINGTIIDFDLVPRIEVEYVYL
                     RDKNGDGDYEDPDDKNYRSTFYVSPVFISGVYNGGYHGYVYRDRNPQALNSITVGGGV
                     PYRSLIRFNTDKLPSDLTINSAVLVLKTEASGNYFRNSSDSINIQAMASTVDTSLWKP
                     GYIEQTTTNSRTDNIRDDTLKLNISSFVQDWATQPNHNNGLVLMNYNIFNGNFNPLYR
                     VRFYNNPSDRDNSPKIIIYYTQSPQ"
     gene            complement(58333..59316)
                     /gene="amrS"
                     /locus_tag="PK937_02105"
     CDS             complement(58333..59316)
                     /gene="amrS"
                     /locus_tag="PK937_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNH31396.1"
                     /translation="MIEAKFYRKEADHIVCELCPRMCKIQEGAEGDCWGYKNIDGKLY
                     SFNYGKIVTAVYDPMEKKPFFHYYPNSTILSLAPNGCNMRCKFCQNHDVALNKKETKD
                     ISLQTILDYSGKSHSIGVAYTFTEPLIWYEFLIDSMRKLKGIGQKNILITNGYLNQKP
                     IEDIIPYIDAMNISVKSMNAETHERLTESKLEIVLNSIKSAFGKTHLELTYLLIPGES
                     DSTEDVGNFVDFVAGINKDIPVHFKRFFPHHKMTSQPMTPNETMVRTYKLATQKLSYV
                     YLTDTDIDGVSDTFCPNCKRLIIKRRDYQSNLGGMRGSNCKFCGHRIFIKF"
     gene            59547..60128
                     /locus_tag="PK937_02110"
     CDS             59547..60128
                     /locus_tag="PK937_02110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31397.1"
                     /translation="MSYQFEHLTPQTWNDFAALFGERGACGGCWCMAWRRSNAEFQKN
                     KGEGNRKAMQRLVKKELPIGLIMYKDVIPVGWCAFAPREEYIRLAASRIFKPVDEKPV
                     WSISCFFIHKDYRKQGLSEKLIRQVIVSAKKMGVQILEAYPSDPYDAQVPAPFVWTGL
                     TSAFVKAGFKEVARRSKTRPIMRYVVNTTDLNK"
     gene            60148..60843
                     /locus_tag="PK937_02115"
     CDS             60148..60843
                     /locus_tag="PK937_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31398.1"
                     /translation="MIFLLFFIFLAVQSAATQDTLPEFPVHFVVIDGSEEVAAVATLE
                     QMHREIEILNTYFVTEDKRTLVRFRFKSATFFDEIENTACSFVDSANEGMYIGRWANL
                     YKECADTKLRDPRAINFVIFDSYTDAQGWRSRRSRGATYAGVPVVFIDYQALGHKGSL
                     EEHEMGHAFGLGHICDSTLTEDGGQNIMHHNGECPDQKKYRAKYYTGFNAEQEAIIRR
                     AILRHRKKLGLDK"
     gene            60843..61364
                     /locus_tag="PK937_02120"
     CDS             60843..61364
                     /locus_tag="PK937_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31399.1"
                     /translation="MPLSPKIDRILSTVLFLGLCVLSFWMIGWFQPNHDYKAKAVIYK
                     PSIFVYEALRDSSRLKSWWTDWAGSEPINDSAHVIHEIIDSEKSEFVLTDRIAWPGIL
                     LTYGYEDAEKKGFLEYRLEYMGDSTRVTLRHRTRGYSWIQRSLLALRYTDDKLRWRRY
                     LYNFKRMMENDSI"
     gene            complement(61370..>62092)
                     /locus_tag="PK937_02125"
     CDS             complement(61370..>62092)
                     /locus_tag="PK937_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNH31400.1"
                     /translation="AVGIPIIASLNGSSHSGWLDYAKKIEQAGADALELNMYYIAADI
                     AESATSIEHRYLEIARTLRQSLRIPIAIKLSPFFTSFAHFAKHMDDCGINGLVLFNRF
                     YQPDINLETLDVMPNVILSQSTELRLALRWIAILHGRIRADLAATGGIHTHEDVIKAL
                     LAGASVTMVCSTLLKNGIARITDIVKGMETWLVEREYESVKQLQGSMSQKSVSDPTAF
                     ERANYMRALSGYNLPNDLLYHN"
BASE COUNT        16577 a        13302 c        14793 g        17220 t
ORIGIN      
        1 aaaaacactg aaaaacttga aattggtttt ggggctaatg gaagtataat cctgtatgtg
       61 ccagttttct tgtccttccg gacttaccat agcgtaaaat cggcgccctc ctactttgaa
      121 attcatatac tttgtttttg aagcgtaagg tttcggcgcc caccattggt caagaatttc
      181 tttctttgta aaggcgtccc aaacaagtga aagctcagca tcaaattctt tttccacgaa
      241 tacagttttg gttgatttgt caaccttaaa gtcaaattgc aaattattca ttttcttttc
      301 cttttcatgg ttgagagtag attatcaagt tgactaaacc ggctttccca tatttttcga
      361 tattgttcca gccaatcgtc aatttctttc attttttcga tttccaaaga gtagaaaatt
      421 tctcttcctt tgtattcttg ttttaccagt tcgcattcgg ttaatatccg caggtgtttg
      481 gaaacggcct ggcgtgtcga attaaaattt tccgcaatag cattgggtgt catcgcttgc
      541 agcgcgatga ggccgatgat ggctcttcgg gtcgggtcgg caatagcctg aaatacatcg
      601 cgtctcatag gtttttctta ttcagtaatt gagttaattg ttcgaagcac agagtaatac
      661 cttgttcaaa gcccatttca accagttttt ggatatccgt ttcggaggca taggaggttt
      721 tgaactcgac ttttgttccg ctttccgttt tagtgaaagc gattcttcct tttgagatag
      781 gaagttcgcc gttcaaatta ccattttcat cgcaaaaaaa gtcttccatg tcaatgaagt
      841 ggtgaagatc aatcgcaata taattcatgc gcccccaatg cttttgactg tcggggccta
      901 ccatggcata cagccaatac cctccgggtt taaaaagcat tgttttggtt tcggctctcc
      961 atggagaagg tccccaccac tgatccaata attctttctc tgaaaaggcg cgccagacat
     1021 taggtaatgg ggcattaaat tcttttgtta cgaggatgaa tttttctttc aggtttttaa
     1081 ttacgttgac aggattactc atatttactc ccgggtttct tcgttgtgaa taaaagttac
     1141 ttaatcatgg ctatgtattt ttcgcggtta tcgtaaataa taaagaggtt gatcgctaac
     1201 aatagaagcg cgatcggtaa tccggaaggc gccacggtaa tatgtgtcat tacaatgccg
     1261 atcatcacgg ggaaaataat gatagcgccg aggggcctga attttgaaat gataaaaaga
     1321 attcctccta tgatttcaac cgtagccaca aggggtaaaa gccatccgat ttccaaaaac
     1381 gcggcagact gttttaccat cttttcaggt aaatcgggtg gaacgggtat gtaattgaag
     1441 aatttattca gcccggcgtt gataaacatc aacccgaaca agaggcaaac cgcaaaaaat
     1501 acttttgacc tcatatacat gctttcacgt gtgtacaaaa aatgcgcaac ttattggttg
     1561 caatataaat gaaactattc ggttgcgcaa gttttttttc aaataaagtt ttgaaaagga
     1621 taatccattt ttatcggttg ggtctgtgat agtttaaaag catgtagttt atacgcttct
     1681 attgtgtcgt ttcgattata ggacggtatg tcttggtatc ggtcataatg cacgaattgt
     1741 atttaaaaaa acttcttcgc ttttatcagc caaaatgcat taactttgtg ggatcatcgt
     1801 ttacacaaac ctacctgttt atgatacgct ccatcaaaaa actcctcgtc gccaatcgcg
     1861 gtgaaattgc gattcgcatt tttcgtgcgt gcagcgaaat cggcattgaa accattgccg
     1921 tttatacata cgaagaccgc tattccctgc accgatacaa ggctgacgaa gcctatcaga
     1981 tcggtgcgga cgacgatcca ctgaagcctt atctgaatat tgatgagatc attcaccttg
     2041 cgaaaaagat cggagcggat gcgattcatc ccggttacgg ttttctttcc gaaaatgatc
     2101 aatttgtcaa agcgtgcgaa gatcacggga ttatttttgt cggacctaat tcggaaatca
     2161 tgtacaaact cggtaataaa attgctgcca aggtagtcgc gcgcgaaaat aacattccga
     2221 tcatcgaaag taatcgagag gcgttgaagg atacgtccgt ggccgaacgg gaagcgcagc
     2281 gcatcggata tccggtgatg atcaaagcgg cggcgggcgg cggcggacgc ggtatgcgcg
     2341 ttgttcgtaa tgcagacgag ctacgggcgc ggtttgatga agcacggggc gaagcgcgca
     2401 atgcgtttgg tgacgatacg gtgtttatcg aaaaatttat agacgatccc aagcacttgg
     2461 aagtgcagat cgttgcggat catttcggca atgtcgttca tctgtttgag cgtgactgca
     2521 gtgtgcagcg gcgtttccag aaagtcgtag aaatagctcc ggcgcttacc gtcagccgcg
     2581 atgcccgtga acgtctttat cactatgccg tacaaatcgc aaccgccgtg ggttaccaga
     2641 atatctgtac cgtggagttt ctcctcgaca aagaagagaa tatttatttc atcgaagtca
     2701 acccgcgcat tcaggtggaa cataccgtaa cggaaatgat cacgggcgtg gacttagtca
     2761 aaacgcaaat tcttatcgct cagggttatc cattgagcga tgcacggctc aaactggacg
     2821 atcaatcgcg tatttattac aacggctatg cgatccagtg ccgcattacg acggaagatc
     2881 cgagtaacga attcaaaccc gattacggta cgatcttggc ctaccgcagt gcggcaggtt
     2941 ttggtattcg attggacgga ggcagtatcg ggccgggtat tcgtatcagt cctttttttg
     3001 attcgatgtt ggtcaaagtc tccgcgtggg gtttgtcttt cgaggaaacg tgcgatcgta
     3061 tgtaccgtac attgatcgaa tttcgtatcc gtggcgtccg tactaatatt ccttttctgg
     3121 aaaatgtcgt gcgtcatccg atttttcgtg aaggccgtgc aaccatcaat tttatcaaag
     3181 atcaccccga actgctgcag tttgtcaaac gccgcgatcg tgcgacgcgt attttgcgtt
     3241 atatcgccga tgtcgttgtc aacggtaatc ctgatgtcaa aatacgcgat gacaaaaaag
     3301 tattcatcaa accgcgtatt ccggcgtacg accgttacgg tgatttcccc gacggggcca
     3361 aacaaaaatt agacactctc ggcccggaaa agtttgcccg atgggtgatg gatcaaccga
     3421 aggtgatgtt taccgattgt acgatgcggg atgcgcacca gtcgttgctt gctacacgca
     3481 tgcgcacgta cgacattatg cgcatcgcgg aaagctgggc taaacatcat tcgaatatgt
     3541 ttagcatgga agtgtggggc ggtgcgacct ttgatgtggc gttgcgtttt ttgcatgaag
     3601 atccgtggga tcgtttgcgt cagatgcggc gcgcgatgcc caatacgctg ctgcagatgt
     3661 tgattcgcgg tgccaacgcc gtcggctata cggcttatcc tgacaatctg atcgaacgtt
     3721 ttatcattga ggcggcgcgg gaaggcattg atgttttccg catttttgat tcgctcaact
     3781 ggacggaagg tatgaagctg agtatcgaaa ctgtcaggaa taaaaccggt gcgctcgctg
     3841 aagcatgcat ttgttactcc ggcgatatcc tcgatccgaa gcgcagtaaa tattcgctca
     3901 catattatgt cgagcttgcc aaagaactcg aacgtatggg cgcgcatatt ttggccatca
     3961 aagacatggc gggattgcta aagccgtacg cggcggacgt tttgatacgc gctcttaaag
     4021 acaatatcgg cattcccatt cacctccata cgcacgatac gtcatccgtg caagcggcta
     4081 cgtacgtcaa ggctatcgaa gccggcgtag acgtagtaga tgtcgcgctc agcgcattat
     4141 ccggcacgac gtcccaaccc aattttaatt cgatcgtgga aatgttgcgt catacggaac
     4201 atcaagtgga tgtggatatg acttcgctta acgccttttc caattattgg gaggctgtac
     4261 gcgatattta ttatcccttt gaaacggatc ttaaagccgg aacggccgaa gtatataatc
     4321 atgaaatccc gggcggacaa tacagcaata tgcgcccgca agcgatttca ctgggtctcg
     4381 gtgaaaaatg ggaagaaatc aaacaaacgt atcatgatgt aaatatgctc ttcggcgaca
     4441 tcatcaaagt aacaccttca tccaaagtgg tcggcgatct ggcgcaatac ctcgtggcta
     4501 ataatatgac cgccgccgat gttttgatga aaggggaaac gatgtccttt cccgaatctg
     4561 tggaaggatt ttttatgggg catctgggac aaccgtacgg cggttttccg gaaaaactac
     4621 aaaaagtcat tatcaaaaat aaaacgcctc tgaccgatcg cccgggtaaa cacctgccgc
     4681 ccgtggattt tgataaagag tttgaagcat tcaaacaaca gttcgatgcc aatctttcga
     4741 ttacggattt tttgtcttac aaattttatc ccaaagtgtt taccgattat tttgaaaatc
     4801 agcaaaaatg gggtcaggtc tctgttattc cgtctgaaat attcttcttc ggtatgaaac
     4861 ctaacgaaga agccatcatc gaaatcgcac agggcaaaag tatcatcgtg cggcttttgt
     4921 cggtgagcca ggcggataaa gacggaaatc gcaaggtgtt tttcgaactc aatgggcaag
     4981 cccgaacgat cactgtacag gatcgttctc ttaatgtgaa gcgcgtacaa aacagaaaag
     5041 ccgatgcctc caaaccgggt gaattggctt cgcctttgcc cggcatgttg tctaaagttt
     5101 tggtcaagcc cggggatacg atcaaaaaat cacaaccgct atgtatcatt gaggcgatga
     5161 aaatggagac gaccgttaca gccgttcaag atggtgtagt caaagaaatc atattagggg
     5221 taaagactat cgtggaaagt ggcgatttgc tgatgatttt ggggtgattt gtgggtgcgg
     5281 aaaatgaatc ggatacttaa gccgtgagtc ggcagctttc gagaataata ttttccgtgg
     5341 atgttttgac ggtgggtgtg atcgccgata cgtcatcgga taaaaactga acggtcccgc
     5401 gcttccaacc ggaaatcgta agcaatgctt tttcatcttt gtcatcaccg accgtcgcac
     5461 ttaaaatttc accgttacga acaatgatct ttccttccgg ataaaattgc cgaatgatcg
     5521 taagcgtccc tgtttttttc tgaagatata agtactgcaa cgtttcgata aaactcaatt
     5581 cacccaaatc gcttttgaaa gtatcggagt tttgtgagcg gatttcttcg attttgcgga
     5641 tatgtttgag gatttcattg accttgccgt tgatcgtatc gcgattgatt tccgatttga
     5701 tcaaatactg atctgcgccc atacggaagc cgcgtttctg tgtaatatcc gaatccatcg
     5761 aagtaaggaa aataaaaggc accatcggga catccgaacc ggtccggact tgctggcaca
     5821 tggtgatacc gtcaagtttg ggcatcatca catcggaaat gacgatgtcg ggtttgtcgg
     5881 ttttgatttt agcgagacct tcttcgccgt ccatcgcatg aagaatggta aacgccgatt
     5941 gctccagatt catcgtcagg agtttggaaa tatgcggatt atcgtcaacg atcagaatga
     6001 cggtcggctt catgaaattg ataaataata attttaatga aagcggtgaa cgaacccgcg
     6061 ggaaactata cggagtcgtt cacaaaaaca agcaaatttt ggcgccatgc acacttgcat
     6121 ttcagacaaa atactctcac ttttcgtgca agaccttacc gggaagcgaa tatgccgctt
     6181 attgacggct ttggccgttc catagattat cttcgcgtgt ccgtcaccga ccgatgcaat
     6241 tttcgttgtg tgtattgtat gccgcacgat ggcgttcagt ttatgccgcg tgaggaatta
     6301 ctgacttacg acgaactcga acgtatcatc ggcgtttttg taaatctggg tattgaaaaa
     6361 attcgcatca ccggcggtga acccacggta cgtaaagaca tcatcgaatt ggtacggcgt
     6421 atcgggagtt tgccgttacg atcgtacgcg atgacaacca acggttattt gttggacgaa
     6481 ctggccgagc cgttgcgcgc tgcgggatta cagcgcttta atatcagttt ggatacgatg
     6541 cgccgtgatc gttttcaaaa aatagcacga tttgacggat atgaaaaaac gtggcgcggt
     6601 atccggaagg ccgcggattt ggggtttgca ccgatcaaac tgaatatggt ggtcttgcgc
     6661 gggatcaacg acgatgaaat ttttgatttc gtggaacttg ctcgggaacg ctcgtggcac
     6721 gtgcgtttta tcgaatacat gccttcttcg ggtgatctcg gtaacacgtt tgatgcatcg
     6781 cacattattc gtacggacga attgaaacgt atcgtatctc agaagtatgc gttgatcccg
     6841 atcacggaag ccgttcagtc cggcccggct aaaatgcata cgatcgaagg atttgcaggg
     6901 aaaatcgggt ttattgaccc gtatgccgaa catttttgtg cggcatgcaa tcgtatccgc
     6961 ctcacagcgc tcggtaaact caaatggtgc cttttttcca acgaaggtct cgatatcaaa
     7021 tcatttattc gttccggtaa atccgatgcc gaactcgaag cgtggattcg tgagcgtatt
     7081 gttcgcgata aaccggagca ccatccgatc gccatttcag atatgattcg cgcgaatact
     7141 gttttttcac aagtcggcgg atgatgtgct aagtgttaga ttggttgtag atcgtgagct
     7201 taagctcttc tcttgccggt atatcaaaaa atccatgaca cgccttacat gaaaaaacct
     7261 tccattctca tcattggcgg aggcatcaat ggtgccggtg ttgcacgcga tgcggcttta
     7321 cgcggatatc acgtcggact ttttgaaaaa aacgattttg cttcaggcac aagctggaca
     7381 tccagcaagc tgattcacgg cggattgcgg tatttggaac acggcgaaat cgggttagtt
     7441 cgcgaatcac tgcgcgaacg tgaaatcctg ctgcatcagc atccgcatct ggtacgtcct
     7501 attcgtttga ctgtgccgat atacaggcat ggtaaatatg gccgactggc catgcgtgcg
     7561 gggatgtggg catacgatct tttgtcgttt gacaaatcgc ttccgcgttt ttccttatat
     7621 acccatcgcg ccgtgcgtgc gcgttatccc ggcatcaatg cgcaaagtct tctcggcggt
     7681 gtttcttatt ttgatgcaca ggcgatttac ccggaacgtt tagttttgga caatatccat
     7741 tcggctgttc gctacggcgc agaagtatat aaccatacgg aagttaccgg atttttgaag
     7801 tcaaaagaac gcataaccgg agtgcaaaca cgaaatacgc tcaccggtga agaagcagaa
     7861 tacacagccg atatggtgat caatacgtcc ggtccttggg tggatcatat tctcaaacac
     7921 accgaaaaaa atataccgcc ccaattaggc ggtaccaaag gaagccatat cgtcatacgc
     7981 aatacgatcg cattgcaaga tgcgctttat gttaatgccc gtcgggacgg gcgtccgttc
     8041 tttatcatac catggttgga ttacattctc atcggcacga cggatttgcg ctacgaaggt
     8101 catccggata cggtgtttcc tgaatcgcat gagattgatt acctcatcga agaagcaacc
     8161 catgtcatgc ctgagttaat ctcagggcgt gacgatattg ttttttcatt ttgtgggatt
     8221 cgcccgcttc cacgtgacga acgtcgtcat accggagcca ttacgcggcg acatgggatt
     8281 ttggatcatc aacgcgaagg cgcggaaggc ttgatttcgc tcatcggcgg aaaattaacg
     8341 acataccgta gcgcggcgga gcaaatggtt gatcatatcg aaaaatcgct tggtattgct
     8401 atgcaaccgt gccgtactgc aacagaacct atgcaccctt tgatgcatca cgatacagaa
     8461 ctgttgccat tgacagcgat gctgggatcc gatgcgggag cggccgcgca aatggcgcag
     8521 caaaaaaatt ggtcattgag cccggttgct cccgattatc aaaccagcga agcgcagata
     8581 cggtatgccg tgcaagaaga atgggcacgc acactctccg atgtaatgtt acgacgtacg
     8641 cgactggctt atagtccgga taacggattg ccggtggcgc atggtgtggc cagtattatg
     8701 gggagcgcct tggcgtggcc tcaggagcga accacagcgg aaatacaagc gtacacggcg
     8761 tatgtcaacg cctatcttca ggcgcatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnac agcggaaata caagcgtaca cggcgtatgt caacgcctat cttcaggcgc
     8941 atcgtcatcg gtcataagtc ttgattgaca tacgcgtctt ccttacatta cctcccgata
     9001 tcgtttctca cttttcagcc tgtagaatta ttgatgaagt tttttcgatt attattttta
     9061 ataaccgtgc tcatggaaat taataccccg gtttgggccg gtacggatga agcgctgaac
     9121 ctcgccgaga aggcgcgtca tgcgattcgc gcatcacggg gtaaactttc agcctacaaa
     9181 cttaccacgt acagcaaatc ttttattgat ctctataaac gcaaaaaaaa tgcgtttgaa
     9241 atgaaaagcg catcgctggt gcacgcggaa tactattggc gccaacctga tgaagtgcga
     9301 gagatcgaaa tagctcgccg ccacattgat gacttttttt atacggatta taatatcgaa
     9361 catcttccga ttattgatga tcttacccgt gcgaggatca ccgtgggtcg cgcatcggta
     9421 gcaggcccgc tgacggaaga tgccgcggtt tactatgatt tttcgctggc cggggacacg
     9481 gtcatcggag gtgttgcggt ccatcagtta cggtgtaaac ccaaaacgga atacgaaccc
     9541 ttgatggatg gggtggtgta tatcgcgcag gaaacatttc aagttgtggg ttttgaaata
     9601 cagtttaata aagccgtcaa atttttcccg cctccggatt tattgcaagt gcgcgtcatg
     9661 tatgcggaaa cgccatcggg tgtttggctg ccgaagcata ttcagtgggg aatgcagttc
     9721 aatattacat ttccgtggcc ggctcaagcg cagtggcgca gtgaaacgat ggtttacgaa
     9781 acagcgatca atcctgaaat accgtcggcc gtgtatcgag gcaaagccgt cgaaacgcgt
     9841 gaggatgcgt atttccgcga ttccgcattt tgggctgata atgaaaaatt gccgatgacg
     9901 gaggatgaag cacaggggtt tagcaatctt ggccaattgc cttggaatct gaaaatcctc
     9961 aatcccgatt tgaaaggata ccgcaaacgt cagcggcaaa aggatattga ttgggggttt
    10021 cagctcattc ccgatatccg ctacaatcgt gtcgaaggat tttttaccgg tgccaaaata
    10081 gaatttgatg atttggcgta caaacgtata gtcaggaatc tgacgatcaa aaccaaagtc
    10141 ggatatgggt ttgcggatga agcggtgaag tattccgtcg aagcgcaaaa aaaatggttg
    10201 gacaaacgat tttatgtcgg aggtaaatat tacgacgatc ttgcgtacaa agaatttacc
    10261 aacgatggca gcgtactcaa taactccctg acgagtctgg tgtaccgtta tgatatttat
    10321 aattattttt atgtacgcgg ttatcagact tataccgggg tcaggccttt tcgcggtgca
    10381 cgggtagagt ttgcttacac cgatcgctgg gattccagtg cgaccaaaaa tgcacactac
    10441 gcgctcatca aaagtatgta taaagatttt gatccggtgt atccgatcaa tgacggccgt
    10501 ttacgtcgta tcacggtgag tgctaactat actatcggca cgggaacagg tatcgctccg
    10561 cggacaccgt actggatttt tgatgcgagt attgatcatg ccaatcccaa ttttctgaaa
    10621 agcgattttc attttacgtt gtataatttt tccgcgcggt ttcatatccc cacgacgcgg
    10681 cgcggttcac ttgacgggaa ggccgcactg ggtctgggca cggaccgatt gccaaatcag
    10741 tacctttttc atctttatgg cggcacgacg ccgtacgttt tgaaaacggt agatttcaga
    10801 gaaccgacgc ccaatcattt tcaaggcaat cgtatggcgg cgttgacgat ggaacacaat
    10861 ttcggcggca cgttattgga acgtacggat ttgccgtttc tgcgcgacgg atatgtagat
    10921 tgtataccga cgttttccat aggttttgta catgcttcta aaaaaacgtt ggaaaattta
    10981 cgacaaaaca aaatccagta cctaagaaga cctttggtcg aagcgggatt tgcggtcggc
    11041 gatatatatc gtattgtgcg gctggatttt acctggcgct tgacgcaacg cagtaaagga
    11101 aatcgtaatt ttgccgccac gatggccgtc ctagtacagc gcttctgacg aaaaagggtt
    11161 tgtttcatat cgcaaataga actaactttt tcgcactttc tgaatacctg taattttttt
    11221 catatccaaa atcgtctaac caaacgggag tatcgtcatg gaaaaacgtc ttgccggcca
    11281 aaaagccctc gtcaccggcg ccaatagcgg catcggtgcg ggcatcgtac aacgtcttgc
    11341 cgccgaaggt gcggatgtta tgatcaattg gtttgccaac gaagatgcga ccaaagcgct
    11401 ggttgataaa atcaacaaag ccggcgccgg caaagctatc gcgtttcgcg cggatgtatc
    11461 caacgaagat caggtcaatg cgatgtacga agcgatgttc aaagaatacg gtaccattga
    11521 tattcttgtg aataatgcgg gtatcgaaat tcataatttt ttggaaaacg tcaccaaagc
    11581 ggaatgggat aaagtgattg gtgtcaattt gacgggtaat tttctctgct cccgcagcgc
    11641 gattcgtcaa tttctgaaaa acgaaaaccg tcccaattcc cgttcacgcg gcaaaattgt
    11701 caatatcagc tccgttcacc aagtgatcat gtgggccgga ttttccagtt attgcgcgac
    11761 gaaagccggt atcaatcttt tcgcgcaatc cgttgcgcag gaatacgccg agaaaaaaat
    11821 tcgaatcaat caagtggcgc ccggtgcgat caaaacaccg atcaatcaaa atgtatggag
    11881 cgatcccaaa ggcatggctg atcttatgac caagatgccg tacggacgta tgggtgaagt
    11941 ggatgatgtc gccgcggccg ttgcgttcct ttgctctgat gaagcggact atatcaccgg
    12001 tacgaccttg tatgtggacg gcggtatgtg cctctatccg gaattccgtt tcggcggttg
    12061 ataacgagaa ctagtatgga agattccaaa gaaacatcgc gcaataaccc gtcggaaacc
    12121 cctgagacca cgcgaaaata cgagcgtgag atttcggatt atattcactt tcagatcgaa
    12181 gagacgcagg ctccggagtc ggcgtttgag caaaaagtgg ttccggagtc tattcgcgaa
    12241 cagggttttt ggcgacgatt tcatgttcgc cgcaggatat ttgtttcacg aaccgccgat
    12301 tttttcaaag atacgaaaaa aatggtggtg gtctggatca tcatcatggc attggtggcg
    12361 atcggctcgt atttcggttg ggatcgtgaa atggtcggcg gtgccgtcgt tttttttgga
    12421 ttgatttcaa gtgcgttttc ctggcttgct gcggtgatct tgggcgggat cggtttgatc
    12481 ccgtttatcg gaccggttct tgtggcgatt ctcagttcat cgctcttatg gatcatcaat
    12541 ggactcggtt attttgtctc cgtggtcgct atccgcgccg gtcacggtaa agccgtactc
    12601 aattaccggt tgttggtcat tatttttttg tgcgggatcg tcacgggcta tgtcatagcc
    12661 aagatcgtgc actgaccaaa aaagaattgg aaattttaaa gcgtttacgt atgttgggcg
    12721 caatttgcaa gtaattctaa tttttttata aactgttttg tttttagagg agtgatggta
    12781 tgagcaagtc gattgacgag cttttaaaag atttacaaag caacaaggtc gaagtcaaag
    12841 aagaggccgc gtttgaattg ggcggttacg acgacgctaa agttgtttcg gcgctgatgg
    12901 gtgttctgaa atctcagcac gaagaagtcg gtgtgcgtgc ggcggcagca gagtctttgg
    12961 gccgccttgg taaagtcgaa gcgtttgatg ctttgatgga atgcctgaag tcgggggagt
    13021 acatggtgcg cagtgcagcg gcctacgcac ttggaaaatt gggtgataaa cgtgcagcgg
    13081 atgcactatt ttttctgctg aaagattcgg atcacagtgt ggtgtcgagt gctgcacacg
    13141 ctttggccaa acttcacgat cgtcgtgccg tgttaccatt gatccacatt gtagaaacaa
    13201 aacgtccccg tcagcaagtt gcggcggttc gtgttttggg tgatttgcac gatgcgcagg
    13261 ccgtggcacc gttggtcaag ttatacaaga aaatcagcga tcccgatctt aagcatgcaa
    13321 tcattcgtgc tttggatgat attagtgcgc cgggttgcga agatgtgctg atggaagcgc
    13381 tggacgatag cgtttcgttt gtgcgacgtt atgctgccga tgctctcggt aatatcaaac
    13441 acaaacccgc taaggataaa ctcatgcagt tagcgcagtc tgacacggat ggcgatgtcc
    13501 gcaatgtggc caaacaagcg gccgataaat tataatcgga cgaaatagtt ttttaaaaaa
    13561 gcctttgtga aaaacaaagg cttttttatt ggctaaattt tatcagttgc cgtgcttgat
    13621 gtttcggcat ggggatgaaa acatgaggct tgatgcgcat cgtctacatg gatgagttcc
    13681 ggttttgtgc tgcggcacat gtggtcggcg tgcatgcagc gcgggtgaaa atggcagccg
    13741 gcaggatatt gattggcacg cgggacttgc ccgggaatgc catacagttt cttttcatcg
    13801 gaaatcagac tggggatggc gcgaagtaaa cccgaagtat aaggatgggc gggtttttta
    13861 aaaatagacc ttaccggccc gctttcgaca agtttcgatg catacatcac agccacacga
    13921 tcggccatac cggccacaat tccgaagtta tgcgtaataa gaagcagtgc cataccgtcg
    13981 cgtttttgct gatcgcgcag cacatccaaa atttgcgcct gtaccgtaac atccagcgcg
    14041 gttgtcggtt catcggcgat cagaagtttt ggtttgcatg ccagggctat agctatcatg
    14101 acccgctgac gcataccgcc ggataacgta tgcggatatc ccgaatacac tttttcagat
    14161 tcgtttaatc ccacgcgttc caataaatct aaacattgat gacgtatcgt tcggctgtcg
    14221 gtttttggtt gaaggcggag cacttcggcg atttgttcgc cgcatgtcaa aacgggattg
    14281 agcgccgtca aaggctcctg aaagatcatg gctacttccc gaccgcgtaa gcttttccgg
    14341 tgatctatgc cggagtcata gatttttttg tcttgccaat atattgctcc gccggcgatg
    14401 gttcccgggt aaggcagtaa atccaaaacc gataatgccg taatactctt accgctgccg
    14461 gattccccga ccaaacataa tgtttcttcc ggtgcgatgt caaaactgac atcatctacg
    14521 gctttataga gttggttttc gacatgaaaa tgagccgata agtggcggac cgaaagtagt
    14581 gaaggcatat ggatggaggt taaatttgaa agaaataaag ccaaatatcc tttgaaaatc
    14641 aacggcgctg tgagtatctt tcgcgcgcat aaaggagatt tgatgacaga agtaaataca
    14701 tcgccgattt cattggcgga tttgatttta cccgccacga ttcgtcttga tttgcagggc
    14761 aaaaccaaac aaagtgtgat tgacgaatta atcgaattga tatcggaggc cgggctggtg
    14821 cgggataagt cggcggttcg gcaagctgta atagaccgcg aacgcaagct cagtaccgga
    14881 cttggacacg gtatcgctat tccgcatgga aaaacaagcg gggttgataa actcgtcggc
    14941 gcttttggaa taaaacgcga cgggattcat tttgattcgg ctgacggcgc cccggcaaaa
    15001 atattttttc tactcgtttc tccggtaacc gttacaggac cgcacatcaa agccctttcc
    15061 ggtattgctc gttttttacg catcgaaaaa aatcgtgagc gccttttgca agccgcgaca
    15121 acggatgaag tcttggcgct tttgggaaaa cccgccgtat gatgcaaaag ctcattattg
    15181 ctgcggcatg ttttatatgt attcttacgc ctgagcttac cgctcaaaaa ttactcattc
    15241 ccatggatct gcgtcaatcc gatcatctca aagcttatgg cgtcgcatat acggcgttgg
    15301 aaaataaagt acccgtcgaa tggcttctca actatcgcgg cggatctttt atgatcgcct
    15361 acacggaaac gatggccaac tactgtttag cgcgcggcgt caaaacggaa aatttatcag
    15421 cggcacaggt tgctgtggtg tatgcgacga tcgaaaatga aaacatggaa atcgtctatc
    15481 tcgaaaaggc gccccatatt gccgtttaca cgcccccgga taaaaaacct tgggacgacg
    15541 cggtcactat ggcgctggaa tacgctgaaa taaaatacga taagattttt gatctcgatg
    15601 tattgcaagg aaaacttgcc gaatacgatt ggttgcacct gcatcacgaa gattttacag
    15661 gacaatacgg caaattttat tcagcgtacg gccgtgcggc ctggtaccaa cagcaacaac
    15721 gtgattatga aacatttgcg cagcagatgg gatatagtaa ggtctccaag gaaaaactcg
    15781 gtgtcgtgca ggccatgaaa gaatttatcg ctaaaggcgg ttttatgttt gccatgtgcg
    15841 gggcgacgga tagttttgat gtggctttgg ccgcgcaaaa aaccgatatc tgcgatgtgg
    15901 tttacgatgg tgatccggtg gatatgcgtg ctaatgagaa attagactac ggtcattgcc
    15961 tggcatttca aaattttcgt gtcgaactgg atccgtacca atatgagttt tccagtattg
    16021 atcaccccag cagtaacggc ttgcggctta aaacaccgga agaggattat tttacgttgt
    16081 ttgatttttc agccaaatac gaccctgtac cgactatgct aacgcaaaat catgtcaacg
    16141 ttgtcaaagg atttatgggt caaacgacgg gttaccgtcg tgaattactc aaagagaata
    16201 tcgtagtgat gggcgaagac gcttccatcg gctcggtcaa atatttgcat ggtaattttg
    16261 gcaaaggaac atttacgttt tacggtggtc atgacccgga agactatacc cacgccgtcg
    16321 gggatccgcc tacggatctt gcactgcaca aaaattcacc cggctataga ctgattctta
    16381 ataacattct ttttcctgcg gccaaaaaga aaccgcaaaa aacatgaaat cctcatcgcc
    16441 taaaatcatt atcatcggcg gtgtagcggc gggaccggcg gcagcggcca aagccaaacg
    16501 gatcaatcct cgcgctgaag tcgttttgtt tgagcaaggc gaacatattt cctatggctc
    16561 gtgtgcgatg ccgtatttta tcggtgatgt gataccggaa gcggaaaaac taattcattt
    16621 tacaccggcc tcgtttgaaa aagaaaaagg gtgtgccgta caaatttttc atcgtgcgga
    16681 agcgatatgg ccgcatcgcc gtcgtgtcgt catgcgcaat ctgcaaaccg atcagatcgc
    16741 ggaatacact tacgatgcgt tgattattgc gacgggtgcg cgagcccgtg tacccgatcc
    16801 ggcttggttt cgatatgata atgtatttgc cgtacgttca ctgtccgata gcatgcgtat
    16861 caaaaattac ctcgcatcac gacgtccttc gacggctgtc attatcggcg gtggctttat
    16921 cggtatggag atggctgagg cgttacgccg tcatgccatg cacgtcaccg tcctgcataa
    16981 atcggaatgg ccaatggact cactggaatt gtccggtcgg cgtgtggtgg cggatgaatt
    17041 aaaacgtcaa ggtgtaaaat ttgaaggtaa tattacttca ccggaattgg tagcgggcgg
    17101 tcaaacagta cgtgcggtga tttccggcag cgcccgtttt gatgccgatg tggtcataat
    17161 agcgacaggt tttgaaccta acacggatct cgcgcgggaa gccaagatac ggtgcggtgc
    17221 ttccggcggt atcatcgtag atcatcacct caaaacaaat gcagatcgta tatgggcggc
    17281 cggggcttgt actgaattta aaaacgcact tattaataaa ccgatgtatc tgccattggc
    17341 caatatcgcc aacaaaatgg gttggatcgc cggagaaaat gcagcgggcg gatttgccga
    17401 gtttcctccg gtggtgcgta atacagctgt taaaattttc gacctcgaag tagcttcggt
    17461 aggtctcgat gcggcttcgg catccgcgca cggtctatcc gttatcgaag aaacaatcat
    17521 ggcgcactcc cgcgttagag catatcccgg cgctaaaccg gttatgatca cacttctcgc
    17581 cgataaacat tccaaacgtc tgatcggggc caacctcatc gccgaagaag gcgccgcgct
    17641 gcgtgccaac atattggcgc tagcgatcca acaaaaaatg acgctgcgtc aaatcgcgga
    17701 tatgcaaatg atgtacacgc ctccgtttgc accggtatgg gatccgatcc ttgttgccgc
    17761 caataaaatg atgaagggat tatgatgaat ccgcacgctt ctgacgaatc atcaataacc
    17821 gtcaaacaga tcgaaacaaa gccactcagt cgtccgatgc gtattttgtt tgtcatagca
    17881 ggccacattt tcgtagcctt gggtgtgatc ggtgctattt taccgttgat gccgacgacg
    17941 atttttctca ttctcgccgg atggtgttat gcgcgaagct ccgagcgatt tcattgctgg
    18001 ttatacacca ataaattctt cggaacttat cttcgcaatt ataaagaaaa tcgcggcaca
    18061 ccattgtctg tcaaaatcgg ttcgatctcc gtattgtgga tttctatcgg catcagcatt
    18121 tggacgacgt ccatgccgtg gtttgtgccg tggatattga tcgttattgc atccgccgtt
    18181 accgtccatc ttgcgcgatt aaaaacatta aagaaagaat catcgcatga gtcttaaaga
    18241 tctcccgatc ggaaaaaaat cacccgacga agtgcacgcg atcgtcgaaa ttccgagcgg
    18301 cagtcgtaat aaatacgaat acgacgtaga acttgaaata tttaaactcg accgcacgtt
    18361 gtattcggcc gtgcattatc ctacggccta tggttttatt ccgagtacat tttatgatga
    18421 cggtgatccg cttgatattt tgatcgtggc atcgcaaccg cttcatgtcg gagtattggt
    18481 cgaagcgcgt ccggtcggtg ttttacgcat gcgggacgat aaaggtccgg atgataaaat
    18541 tctgggtgtg gcgatacgtg acgaacagtt tcgcgatatt caccgggtgg agcaactgcc
    18601 gcatcatttg cttgtcgaaa tagaacattt ttttaccacg tataaacatc tcgaaggtaa
    18661 agacgtacgc agttttggtt gggaaccgga ggcgtttgcc aaatcggcta ttatgcgtgg
    18721 tcatcagaaa tatctggaac ggaaatcgtc gcatgcctga acgtacgcat cgtatggcgg
    18781 tgacggcata catcgttcac gatggtaaat ttcttttgtt gaaaagaacc aatcctccga
    18841 tgatttgggg accgccgggc ggaagactca atccggatga aaatccgaat gaaggagtgt
    18901 tgcgggaagt atttgaagag tgcggtctca cggtaaaaat acttcatccg gtggatattt
    18961 ggtacggtga tttcgggcgc gggatctatg tgtcggtgga ttatttagcc atttcgccgt
    19021 cgagcgaagt atcactttca gacgaacata gtgattatat atggtcggat atgaatgcgt
    19081 tgcgtaacgg gcaaccctcc ttgggcagtg cggcaccggc ctttacgatc catgattttc
    19141 gacgggcatg ggaactgtat caaaaactat tataaattat aattgtaata aaggaatcgc
    19201 aacgtgagtc acggtgaaac tattttaata ctggatttcg gatcacaatt tacccaattg
    19261 atcgcacggc gtgttcgcga actgcaggtg tattgcgaaa tacatccgta caatatgacg
    19321 ccggacaaaa ttcgcgcgct gcaacctaaa ggaattatcc tgtcgggcgg accaagcagt
    19381 gtgtatgaaa acggagcgcc gcatccggat cgtgccatct tcgatttggg aattcccgta
    19441 ttgggggtat gttacgggat gcaggttatg gcttattttc tcggcggtgc tgtagataaa
    19501 gcggcacatc gggagttcgg tcgggctgaa attcgcatta ccgatcacac ggatttgttc
    19561 agcggctttc ctgaaaaaac gacggtatgg cagagccacg gagatcgttt ggaaaaaatt
    19621 cccgatggat ttgaaatcat cgccggtacg gacaatgcgc cgatcacggc aatgcgaaat
    19681 aaagcgcgtc gtttttgggg cgttcagttt cacccggaag tggcgcatac accgctgggt
    19741 atcaatcttc tcaaaaattt cgttcgcacc atttgcgcgt gtacgggtca atggacacct
    19801 tcttcatttg tcaaagaaac gacggatgct attcgtgcta aggtcggtga cggttatgtg
    19861 atttgtgcgc tctccggtgg cgtggattca tccgtagtag cgatgctatt gcatcaggcc
    19921 ataccggatc gtttttcggc tatttttgta gataacggac tgcttcggaa aaacgaacgc
    19981 gggcaagtcg aagcggcgtt tcgttctttt tttaataata atctgattgt ggcggatgcg
    20041 tccgaacttt ttttgacgcg actcaaagat gtgaccgatc cggagaaaaa aagaaaaatc
    20101 atcggcgcaa cgtttatcga tgtgtttgaa acggaagttg aaaagttgcg tgccacaacg
    20161 ggtcacattg actttttagc gcaaggtacg ctctatccgg atgtgatcga aagcgtatcc
    20221 atcaaaggac cctcggctac gatcaaaaca catcacaatg tcggcggatt accggaaaaa
    20281 atgaaactgc aattaatcga acccctgcgc gaacttttca aagatgaagt gcgcgccgtc
    20341 ggtcgtcagt taggtttgcc cgatgatctg cttatgcgtc acccgttccc gggacccggt
    20401 cttgccgtgc gtgtgctcgg cgaggtaacg aaagaacgct gtgatttatt gcgcgaagcc
    20461 gatgcgatct ttatggaaga attgaaaaaa tcaggcgagt attccaatgt ttggcaagcc
    20521 tttaccgttt tgcttcctgt caaatcggtc ggtgtgatgg gggatgaacg tacttaccaa
    20581 aatgccgtcg ctatacgtgc cgtcacaagt caggatggca tgaccgccga ttgggcgcat
    20641 ctgccgtacg acgtattggg tcgcatttcc aaccggatta ttaatgaggt tcgcggtatc
    20701 aatcgcgtaa cgtacgatat cagttccaag cctccggcta cgattgagtg ggagtaatca
    20761 tgtatgaaat tgctccgttg gcataacctt gtttttttga tcggaatgat gcacccgata
    20821 gcattgtgtg tttattcaca gaatgaacgc ttgtatttcg atttggttga tctgcctaag
    20881 acgatgagtc ctgccgttcg cagtatcgtg caggatcatc aaggactgat ttgggttgga
    20941 acggaaaaag ggcttggccg ctacgacggt tattcgtggc gagtttttca ttcggacgca
    21001 acggatagtt ttttaatcag tgatgcgaat gtttggacgg tgtttgaaga tcgtaagggt
    21061 tatctgtgga ttggtacgat caatggcggc gtcaatgtat tggatcggaa tcgttctcgg
    21121 tttcatcact ataaacatca acccaacaat acggcttcgc ttagtaataa tacggtctac
    21181 tctatcttag aagacaataa aggcgtgatg tggatcggta cggaaaacgg attaaattgt
    21241 atttatcctg aaagtaacaa ggttatgcgc ctgccggatc aaaccaccga tccgtccggt
    21301 tacaaaggaa aaatcatcca ttcccttgca atggattcgt tgtatcgcat ttggataggc
    21361 agcgattccg gacttaccgt atacgatccc caaacctgtc aattttcagt atttcaggtg
    21421 caagagcatg gtcgcattta tactcctcgc cgtgtgcgca aattgtatgc ggatgaagat
    21481 cgtcaaacgt tatggattgg cactttcgga ggcggacttt atcgctgggg tattcatacg
    21541 cacgagatta agcaatatga tgtcaaatcc gtcgaaaacg cttatccttc aaattatgtg
    21601 cgcaatctcg taacggatcg cgatcacgaa catttatgga tagcaatacg cggcggaggc
    21661 ctcggacgat tgcacaaagc ttccggtcaa atccggtatt ttacacgaca gccaaacatc
    21721 ccgctttcga ttcctgataa tgcatcgctt gcattgtacc gtgatgatct cggcagtatc
    21781 tgggtgggtt tcaattacga cgggcttgcg attatgaatt accgcaagtc ttggtttcaa
    21841 catattcgcc gcgatgcgac cggtacattg gtggatcaca cggttcgttc ggtttgtgta
    21901 gcgggcgacg gtacgaccct ttttggagta cagcgtgccg tacatcgtta tgatccccat
    21961 acagatcgat tacaatcatt cgtgatgccg gaacacgtgg tgaacggttt caatcaagtt
    22021 caggccatat tgtacgataa aggtcaaaac tattggatcg gtacctatgg caacgggctc
    22081 aatcgcttgg atgcggccac aggaaaattt agcaaatacg acgcctatcc ttccggagtg
    22141 caacagcgaa ttttatcact gactcggttc ggggattttt tatggatcgg gacgccgtcg
    22201 gaaattatac gaattcatcc cgctaccagt tcatcaagaa tttataagaa tattagagcg
    22261 gtcaattcat ttgctcagac caatactcaa aaattgattg ctggttcggg gacaaacgga
    22321 ttattttttt atgatgccgt cgccgattct tttatgccat ggctttcttc gtcaatgaaa
    22381 tatccgtcgg attacgaaac gcgttcgctt tttgctgatt cacagtatat cgtatggcaa
    22441 ggtacgtcca atggtttaaa taaaataaac cataaaaccg gagaaataac acgttatacg
    22501 aggaacgatg gaattccgag taccgatgtg accggtatta ttcaaagtcc tgatcaaagg
    22561 ctctggatca gcacgacaag cggcatattg cgtcttgatc ccggtcaatc gcaatttcgt
    22621 tattactata aagaaaacgg tttgctgggt aatgagttca gtttgtcggc tgttgctatg
    22681 agttcggaag ggcgtatttt ttttggaggt attcaaggtg tgacctggtt taatgcctcc
    22741 gatgttcgtg attatgacta caagccgact ctactcatta ctaaaatatc gcgtttctat
    22801 caagatgttg acggcgagat accttataac agcatcgaag cacaatacaa tgaaaacgtt
    22861 ttgacatttg aatactcggt actggattat gcgttttcgg caggtattca ttatgcctat
    22921 cgtttagagg gttttaatca gacatggatt caaaccgatg aaaggcatgc gacgtttacc
    22981 aatttggatc cgggcgaata cgttttccgc gttcgtggaa cgcgaggtga tggaacctgg
    23041 attgattccg aaagtaaagt ttttattact attcatcctc cgtactggct tacatggtgg
    23101 ttttggagcc ttgttgtgtt ttgtatagga atggttacgt ggatgggata tcgtatgcgc
    23161 gtgcgcagtt tgttgcgtcg taagcgtgag ttgcaaaccg aagtcgaaag acaaacgaag
    23221 gaattgtatg caaaaaatat cgaactgcaa tccttgaacg ataccaaaaa tgaatttttg
    23281 ggtatggccg cacatgatct tcgcaacccg cttggcgctg tagggaatat tgccgagtta
    23341 atgttatttg aaatgaatcc ggaaaacccc gaaaccaaac agaccttcag cgatcttaat
    23401 cggattattc aacttacgga acgcatgagt tatctgatac acgaattgct tgattattca
    23461 gcgatcgaat cgggtaaagt ttcgttagcc gtagatacga ttgattggac cgtgcttgta
    23521 cacgaagtat gcgaaatgaa tgagcgcgcg gcatcgcgaa agaagattgt tatcaaaaaa
    23581 gaatttagcg atgggacgat cgtacttcac ggcgatcgca tgcgactcat gtcggtgttg
    23641 gacaatctgg tgtccaatgc gatcaaatac agttttgcgg gtactgaagt attgattcgt
    23701 tccgaagtgg caaatcgaaa gcttcgtgtc acggtatatg atcatggcca aggattgccg
    23761 gaagcggatg tggctcgcgc attcaaatct tttcaaaaac tatcagccaa accgaccggc
    23821 ggtgaaagta gtaccggact gggcctggct attattaaga aaattattga cctccacgcc
    23881 ggacaagttt ggcttgagtc cgaattgcat aaaggttcta cttttggttt ttctctcccg
    23941 ctggagcgcc cgttctccga ttaagtttgg atagtaaatt gccccacctg gtttgcctta
    24001 acactttttt aacattcttt ttacagtagt ctaacactgc cattcgcaaa aatgattatg
    24061 atggcgcagt tacggatgga ttatacgtcc aatcttgcat tacgataaat agccgacttg
    24121 aacatgctcc aacataacaa tcggatccat gaggcgcttt aaaacaaccc taagatgcat
    24181 cggtattttt ttgcgttttt taagactatt taagtcggct ttttttgttt tgcaatggat
    24241 tgccttaacg caaataaaaa aattaatgaa caaatttttt caaagcccaa cataataatt
    24301 cattattaat gataattcat aattaaaggt caaatcctat gcacggaatt gtatttatag
    24361 agctgagaaa atttgtagaa gtgggcttcg gccgtgacgg ttggatgcag atcttaaaga
    24421 aagccgatat cggcgaagat ttgtttatgc cgtctaaaac gtacccggat catcagattg
    24481 tatctatcgt tttggccgcg tgcgaaataa ctggaaaatc agcccatgaa atacttgatg
    24541 cttttgggaa gttcatagtt ccggacttga tcggtattta tggctctttt attgacccca
    24601 agtggacgat gatagatatg atcgaaaatg tagaaaccac cattcaccgt atcgtgcgta
    24661 tgaaaaatgc aggcgccgat ccgccggagt tgagttgtac gcggttagga aaaaacaaaa
    24721 ttgaaatcgc ttataaatcc aagcgtaaaa tgtgcgcatt tgccaaagga ttggtagcgg
    24781 gattagcgaa tcattataac gttaaagcta cagtaacgga gacgctttgt atgcacaacg
    24841 gagatgccga atgccgaatg caagtggtta cctcgtaagt gtgtgctgtt tccgagccgt
    24901 tgattagaag cccggtcttt gctttgcctt aagtacgata taaatagtct aatcaacttc
    24961 gctgcgtaat cattcgtcgc aaatggtttt gattgcagca cggcatgcaa tataaatttt
    25021 gcatctatcg tacgtgttat gccgatctaa aatacggctg tttctttgcc tgcagcttaa
    25081 ctcttttttt acattctttt tacgccgcac taacacggta ctgccgtgaa ttgtttatgc
    25141 ttggatgaat aaatgacgaa gtaagtcttt gtatcaaaaa aagtaatgcc acatgaaaac
    25201 agataacgcg gtgcaacgtc tgcttgaatg tgaatgacta tgatgttttg tttgtgaatc
    25261 aataaattcc aaaattcaaa ggagtaaaaa catgcgtaaa ttgggggtta ttcttctact
    25321 tttgcaaaca cgactcatca tggcacaagc cacggtcagc gatgcacaga tattggcgga
    25381 tgtgcgccgt tcgcgaccgg atgcggctac ggtgaaagtc gtagggtcat cgtggtcatt
    25441 ttataaagat aaagggtatc tttataataa ccgtgccgaa aaaaccgtag atttgacaac
    25501 gaccaaaaat tcggacggtg tttcgttttc gatgcgagga gccgctgttt acgaatcatc
    25561 gggcccggga caaccgtata tgtatagtcg tctttttgtg tttgaaagtt cggtaaccat
    25621 gatcggtggt ccaaattact cggtaacgga tctgcaaaaa atagcatacg aaacggcgcg
    25681 tcgcgatatt ttgaggtggt ttagcaatcc gcaaagtatt gctcgcgttt atgaaatcaa
    25741 agtacctgaa aatcacgatc tccgtatgtc cgcactggat cacatgagtt ttcgggttca
    25801 acaaacggtg ttgcgtgtgg ataatacaaa tactgtgcat aaagaggtcc acgaagacgt
    25861 tctcacattt agaaaaaaca aagacggatg gtttgttaca tcccctctga cctatgctaa
    25921 aaagaagaca tcgctgggca gcatgaaacc cggtactcgt gttttatcgt ccgtaccaag
    25981 tttgcaggat gtaggtctgg atcgtttata tggcgattcc aaagatgatt tttttaaaga
    26041 tggagtggat cagggtgaaa aatcgcctac ggccaaagac atcttttcac gcaataagtc
    26101 gcagaatgat ccatccggcg gtatcacgat tccaaaaaaa aaagatgatg gcgaatccgt
    26161 aacagatctt ccggaggaca cccaagttcg caatgatatc atgagccgag atgcggatgt
    26221 tgcggcggta tcctttatcg gtaactggaa tacacgtaaa accaaagaag gtggcggtgt
    26281 gaatttggca ggtcggatgg tcgtccttaa gaaaaaaaac caaaactcag ataaatttgt
    26341 agttgtttac aacaaagata acggtttgtg gaaattgaaa gcggtcgaaa aaagcccgca
    26401 ataaactcaa ataggcgcca gcaattaacc gataaatttg taacctaagc cgtaaataga
    26461 aatcagccgt tttggtttgg atggtatttt ttcgattttt tgtcggatag cagctacgtg
    26521 tacatcgacc gttcgtgttg tgacggtatc gccgtatttc cagacatgtg ttaataattc
    26581 atcccgtgtt atgatcaacc cttgatgctg tactaaatag tgcagcatct tcatttcatg
    26641 ggcagtcagt ttaaacggtt tattttctaa tttaaattcc ccgcttttaa aatcggcaaa
    26701 taatccggcg tgcttgtaaa cttcagaggg agatactgtt tgtgctcgac cgaatcgtcg
    26761 tagcaaggca tccagtcgcg ccaaaagttc gtcattatga aaaggcttgg tcagatagtc
    26821 atccgcccca agcttgagtc cggctacttt atccggtacg gagtttcttc ctgtgaccat
    26881 caataccggt acattgatat tagcccggcg gatatcacgt acaatatcca atccgtcttt
    26941 gacgggaagg tggatatcga gaatcaccgc atcccacatg ccgctacagg ctttttcaaa
    27001 accaacgctt ccttcatcgc acgtcgcgca gacataaccg gcatgctcaa gcagtttgtt
    27061 gatattacgg gaaatgtccg gatcatcttc aatgactaaa atacgaaaag gtaaaaatgc
    27121 catgaaaaaa aatacaaata ttatgccgca gattcaagca ttccgcacga attgcccgag
    27181 tttattcgtg tgttgcattg ttaattcaga aacggtacta taggcgttca ttgtaaacaa
    27241 aatgctataa gtaccatgac cacattttct gatattcttt taaatctacg taggcaatcc
    27301 aaatccaagc cggccacttt accgaaaaat gaatgtacga cgtaccgcgg atcggtttgt
    27361 acaatatgcc cgctcatcca gtctaattat aatgacgaat tgcgaatcaa aaacgaggcg
    27421 atcagagaat tttggcgcat gcacaggtta gacggaaaac cggagtctat catcgcttct
    27481 ccgctttcgc gccggtaccg taccaattct aaacgccgcg cttttattac ggataatcgc
    27541 gtgttgctgg gattgaccgg gatggatgac gaaggaaaaa tcaaaccttt ggatgtgcgc
    27601 caatgcggta tcgaaccgct tttgcacagc cgcatttacg cctacgtgca gcaatggttg
    27661 tcctccgaag cttcgtatat gtttgcccgt gcgttgaatt atgtcatttt gcgaggcgat
    27721 gatcgccgga tcaccattct cctcagtgtg cgcgatgaag ataatcggat caaacgtgat
    27781 tgcaccttgt tatccaaatc gctgtcgcgt gaatttccga tggttgtcgg tttccttctg
    27841 atcagggatg aatattcaaa atattatatg tccgacaaag ccgtgagcga tccgacagcg
    27901 ttgcaaaaaa tattcggaga acgggatgtc gtccaagatg tgggggagca acagttctcg
    27961 cattcggttt tgggtttttc tcagaccaat ccgtccgtac ttccggattt tatcgcgatg
    28021 gtacacgatc tgatttcgcc gcaaccgcat catacgttgt acgatttata ctgcggctat
    28081 ggtttgttcg gtctgagttt ggcgcccaat gttgaaaaag taatcggtgt agaaatgtcg
    28141 cgtcattcgg tggattcagc gcgtaccaat gcgcttcgaa tgaaaattgc caacgcacgg
    28201 tttcatatcg gcgacctgtc cggtcttacg ctggagccca tactcaaaaa gcagaaaacg
    28261 ccgtttattg cggtattgga tcccccgcgc tcgggaacgg caccgggtgt tatcgagacg
    28321 ctggctatgg ccaaaccgga tcgcgtcgta catgtgtttt gcgacatcga acgccttccg
    28381 tatgaattaa aacgctggcg tcaaagcggc tatcgcgcgg cacatgtgat acccgtagat
    28441 atgtttccgg cgacgggtaa cgtggaagtc gccgtacttt tagagacgtg aaataaataa
    28501 aaaaaaactt gcacaaccga aggtcgtcgc ttatattagg cgcacttttg atgttcttag
    28561 aaaatatggc cccatcgtct aatggttagg acatcgccct ttcacggcgg taataggggt
    28621 tcgaatcccc ttggggtcac cggccaataa aaaaaacctg cttacgataa gcaggttttt
    28681 ttgttttaat caaatgtgac cgctatgatc aggagtaaaa tcttattgct cctgatatta
    28741 ttttacctca ataataggca acacattata tgttatgccg attcctatgc gcattaaagt
    28801 tgtccgtaca tcgctactcg gtccgattac ggtagcttag taattcatga ggggataaat
    28861 tttcaggatt gaccaaacga tagtgttttt cagaaacaac cgtaattcca ttttccgata
    28921 tctcgaactc gaataacgcg atcagatcgt cagtaatgaa ttgcgcacca attggtttgg
    28981 cgactaagtt aggaaatttg cttttacaca atgcgatatc ttgttctatt tgcacaattc
    29041 caagtttatc agcagaacct tttgcttgca ctggaataat gtaatgcacc cctttcttgt
    29101 caatgccaac atagatttca tctgtttcta tttggccgag gtccgaaaca tttgatcgca
    29161 agtggctttg taatgaataa catgctattc cggtaaaaat gtcgattagt cggttatatc
    29221 tgatttttgc caacagtgct tgctcatcag agaaagcata tgactctata ataccaggtg
    29281 tggcattagg gattttggtc tgactcaata tctcagatgg atggatgttt gcaaattttg
    29341 caattacaaa tttgtactta gattgaccgg caggtctgat tatccattcc attcctttag
    29401 gtgccttgga ggcaatcgaa tcaggcagtg caactcgata ccgaaaagtg taaataatat
    29461 cgccgatgtt cttaggtagt ttgatcttta gattttttgc aacgcgttca atttcatctc
    29521 tttcaaatgg tacttctttc atcccatttt taaaatggtt aaagaatatt ttttctatga
    29581 tttgtttata tcggttgtct gatttcatga ttaatttccc ggccatgtca aaacaactac
    29641 ctcttcgcgt aattgctctc ccgttgcagt agcgaatctt gttctaaata aatcaatccg
    29701 atcaactcta taacctaact ttcttgcaat atcagcgaga atttcgccgg ttcggatcat
    29761 tatcctcaag taagaggctt gatctccaac aacataagct aatcgagcgc ctggttttaa
    29821 taccggttgt aattctttta gatgttttgc cattcctcca aaatataata atgtcactct
    29881 actgtataaa cgctcaaatc cagatgtctt attaagctca attcgacgcg cctcgatttg
    29941 gtcggcaata cgcttaattt cggggtgatt ggcaatccat tggtgatcgt catcgtcttt
    30001 gtaaacgcct ctggtattcg atctaatcaa agtcttttta aattgacgca attcttcttt
    30061 gcttcgtatg aagcccaaca aaacggattc caaacgagtg gttcgggtgt aatctttttc
    30121 atttggatat ggcggcgagg tgataaccgc agtgatcgat tgaggtttaa tcaatgcact
    30181 cgcctgacgg gaatctccca aatgcgcaac ggattcttta ggtttgccga tttggattga
    30241 tcggatgtca tttgcgattg catttatttc aaatagccat gatgcaatga caggtacatt
    30301 cttctttatt tttccaagac ctacttccgg gccaaatttt aggttacttg ttttaaacac
    30361 tgtggccttg gctaacccta atagtaaatg aggataatat ttcttttggg catgattctt
    30421 aatcttttct aacaacacta acgttttgtg tagtggaatt gggcttatag aatttgttaa
    30481 aatgattttt tctaattcgg aatcaagtgt tcttagagga aatgtatgtt ctggcatatt
    30541 aatgtcatca tcatcaattc cacttttttc aaattctgat ctgactagat cagctatttc
    30601 aagtgcgcat tttaaaaatt gatcagcaga tatttcccaa ttgattttag tggtcgatgc
    30661 aaaatgagcg aacggattag cttctatgcc tacggagtta attccattta gctttgcttc
    30721 taccagagtc gttccggtac cgcaaaaagg atctagtaca atatcgtttt gactgctaca
    30781 aaactcttgg agataggttc ttaccagatg cgcgggataa gacaatacaa atcgatacca
    30841 atcatgaaat cgtttatcat ctaagttgag cttgttattc agtgggctaa acgggcttcg
    30901 cttaccgttt atttggttat cccactcaac aaacgagtta tacgcaaact gattcgatgg
    30961 catcggtttc ctttatagag tatatgtgac gcttgccaag atagttaaac cgacttcgtt
    31021 gttccacaga aattttaatg catgtattga atgccgaagc atcattttcc ccttgattaa
    31081 gggcgcatag ttccatcgta gcattataac agtcacaacg tagagtatat gaactatgtc
    31141 gtttgaaatt gctcaatact ttgaaaaaag ttgtgtaata gtgattttgc gacttcccac
    31201 caataaaaaa acctgcttat cgtaagcagg tttttttgtt ttatcgtgat ccgtcgtttt
    31261 attttttggt cagctgacgt ttggccatac ggcgctccaa taaagattga agtcccttgg
    31321 gtgtgccggc cgctgaattg atttccgcat ataatttttc ggcgtattca aactgctgct
    31381 cttcccaata catcgaagcc aatttgtatt ttaaccgcag aacgtcgctg tggtccggga
    31441 agcggttaag ccattcctgc agaagcgcca tcgtggacgt ttctttggga ggggcatttt
    31501 cagtgatctg tgtgaatatg aatcgcactt cacgaagcca cggtgaacgg gggtacatgg
    31561 ccattatttg ccgggcctgt tccaacgcgc gctgaatgtt gttttctttt tggtagttgc
    31621 ggataatgcg aaccagcgct gcttcgacgc gataattgcc gtcggtgtat gttttataac
    31681 gttcggctag gtcaaaataa atcggcatgg ctttggccga ttcgcccgca ttttcaaagg
    31741 cacgggcgac acggtacata cgcgcatccg ttgccaaggt agtgaccgga ttatcggtac
    31801 ggatttttgt gtacaattgg aatgcatcat cccagcgttt ttccatttca tacgtatttg
    31861 ccgttttgta ggctacccat tccgtcagat cgccgaagat atttaccgat gcatagatgg
    31921 tacggagttt gtcatatgcg cgtatattca aaagtgaatc ttcttcggca agtaccttgc
    31981 gaatcagcaa tacacgatcg ccgtcacctt cggccttgcg aatatgtatg cctttatcgg
    32041 ttcctaccca cacggtctca tctttggtaa aaagtacggc tttgacatca ttggacggta
    32101 atccggtttc cgtggtataa ctgatccaat aggtaccnnn nnnnnnnnnn nnnnnnnnnn
    32161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32221 nnnnnnnnnn nnnnnnnatc attttgggtt ctttgtcaaa ctgtaccaac ttgttgtcgg
    32281 agatcagcca caaaataccc tgactgtcgt cggcgataaa ttttacttta tcggacggca
    32341 aaccgttttt ggccgttaca aggcgaccgc ctttaccgac gatttgcaat ccctgatcgg
    32401 tagcgcaaat cgtagtttta gttttagcac gataaagtac ctgatttatc gtttttttcg
    32461 cgaagggcgc gggttgtata atccacttgt agcgattctt gctgtaattc atgttataca
    32521 aattactctc aggcacgcct tcgatcatag cccacagatc acgatctgca tccatgtaaa
    32581 acgattttac cgtcggtgtt ttcagtccgt cgctgtagga ggtgagtgcg ttgagtttga
    32641 tatcccattt gacaacaccg ttgcctgatg tggacgccca ggcaatgtcg ccgtcttcga
    32701 atatggaggt gacggcagaa ggtgaaaaca gcgtttgcca tgtgatgctg ccttgcaggc
    32761 cttcgagtat atctttgcgg tgctgggtat cataaagcgc cgcaagttgg tactgtactt
    32821 caaagtcacg tgtcgaatta gctaaggcac ggagttcttt agatgcgacc ccggcagagt
    32881 ttccggcgga gagtatccgc cagcgaattt cgtcggcacg cggattggat gggaattgtg
    32941 tgttaagatt ttgcagcgtt tgaagcgcac tcaccgaatc cccggcgagt tctaagaggt
    33001 tataacgcat ccacgcaatg gtaaaagcgt ccgcatcatc cttgtagagg gtgagaaatt
    33061 tctgatacgt agcagcggat tcagcatatt tttttatctg tgactgcagt tgtgccgatt
    33121 tcaaaagtaa attttttgta cgtgcatcgt tcggaaagcg ctccgtaaaa cgaagatagg
    33181 tgtcggcggc acgttcgatc atagcttcgc gctcgtagag ttcgcccaac ccgatcattg
    33241 cggtcgcggc ttccggtgtt gcggataagt acgaccggtt gactatatga ttatagtata
    33301 aacgggcacg cgtattgtcg ttggaccaaa atgctttttg cgcctcggta agaaaataag
    33361 gagcgatgcg actccctatg gtttcatcta aaaaagtttg aacgccgagc tggtggcccg
    33421 cccatatcgt attgaacgcg tcgcacgtga cggcatatcc ttcgtcggat accagcattc
    33481 cttctttagt cgaatacttc acgacacgcg cgccgtcgat cagatgcaca ccttcatttt
    33541 tggtggcgac ccagcagcgt ccgatcttat ccaaagtcag ggcgcgaacc ggtatatcgg
    33601 agagaaattg cgtcatcaat ttcccgtcaa ataaagcgac gccgcgtgaa gttccgatcc
    33661 acacgttgcc ggaggagggg tccgcgacca cgtcaaatac gtcatcggac ggaaggttat
    33721 tcagtttgcg tgtatagagc gcccattttt tgttgtagta tgaaaccaag ccgtcatcgg
    33781 ttgcgatcca aaccggacca ttccaaaccg aactgattcc gcgaatggtg ttggacggaa
    33841 ggccgtccaa agtcgtcttg cgatctaaaa gcgtccaact gttgccgctg atacgtacca
    33901 atcctttttc cgtgccgatc cacacactac cttcttcatc ggcgtggaca ctgttgattt
    33961 cgttggtcgg caaagtttcc ggacttttga ggtattgttt gactttcttt ccgtcaagaa
    34021 cgttaaggcc ttcttccgtc gcgatccatt ttcggttttg gttgtcgatc gtgatagcgc
    34081 ggatgatatt ggacgtcagc ccgttgcctt tgtcaaaata aaatttctcc gttccgttaa
    34141 cgacgccgcg aataccatcg ttggtgccga tccaggttac cgtatcttcc acggcaatgg
    34201 ccgttacagc tgttttaacc gataaacgcg gccactgggc ggaggccgat accgcagcac
    34261 acagtgataa aactaatgca ataatcctct tcatagactg tattttcctc ataagaattc
    34321 gtttttttag ctgttttaat tatacctttt ttggcctata attgcaattc ataacacatg
    34381 tttgcgagtt taatttttta gggaatcctt atatgatacg ttggttgacg tcaggcgaat
    34441 cgcatggttc gggattggta ggcattatag acggtgtgcc tgcgcatctg aagttgtcgg
    34501 ttgatgtcat caatcatcag ctttggcgcc gtcaacaggg ctatggacgg ggcggtcgca
    34561 tgaaaatcga aaccgatcag gcggagatta tttccggtgt tcgttttggt gaaaccatcg
    34621 gtgctccgat aggattgcgc attcgcaata acgactggga aaattggacc gacaaaatga
    34681 tgacggaaac tccatcgccg gagattttga aaaccgtcaa gccggtaact ctccccagac
    34741 cgggacatgc cgatttggcc gggatacaaa aatttggttt tgatgatata cgtccggtga
    34801 tcgaacgatc cagcgcccgt gaaacggcaa tgcgtgttgc gttggggacc gtggcgcgtg
    34861 aggttttatt atcgtgcggt attcgtatcg cgtcgcacgt gacgcatatc gggcgcgcat
    34921 ctctgaccgc ggctgaatcg gagaaactcc gcgtggatac gatgacgacg gaggcgatta
    34981 atcaaaaagc cgatctatcc tccgtacggg ttttggatgc cgaagccgaa caacgtatga
    35041 tcgccgtcat caaagaaacg caaaaacaag gcgactcact cggtggaatt tttgaagtgt
    35101 atgcggatgg tttgcctgcg ggaatcggca gttatacgca ttgggataaa aaattagacg
    35161 gcgtgatcgc gcagcatatg gcttcgatac cggcgattaa aggtgtggaa atcggaaacg
    35221 catttgataa cgccttgcaa tggggttcgg aagtgcacga tgcgattttt tatgatgatg
    35281 ttcggggata ttaccgtaaa accaatcgca gcggaggatt ggaaggcgga atgacgaccg
    35341 gtgaacggat catcgtgcgc tgtgcgatga aaccgatacc gacattggcg cggccgttga
    35401 tgtcggttga tatccgtacg catgaagccg ccagtgcgca taaagaacgc accgatacgg
    35461 tatcggtgcc cgcatgcagt gtcgtggcgg aatccatgtt ggccttggct ttgatcaatc
    35521 ccttgctgga aaagtacggc ggcgacagta tgaatgatat ccgtacggcg atggggcggt
    35581 agcttatatc gttaaattag aaacagttaa gagactctta agttacgatc atccttagcc
    35641 gatgaacgaa attttgaaaa aaattgaata ttttttcaaa aaacaccgtt ccatccttga
    35701 cagctctcgt tttgttgctt aacttttatc ggtgcacata atataccggc cgatcaatcg
    35761 aattgattga tttttaatga tcagacaaca cctacacgat ttcctcaaat atctcgacgc
    35821 cgaacgtaat ttttccgata aaacggtggg cgcataccat acggatgtcg cagcatttat
    35881 ttcgtttgtt gagacgcgct tcgacagttc atcatttgat gcatccgata tttccaaaaa
    35941 cgaaatacgc gcttatcttg gtcaactcag tcgtgatcgg ttgcaaaaaa aaagcatcgc
    36001 acgcaaatta tccgccgtaa aatccttttt caaatatctg accaaacagc aaatcattac
    36061 ggccaatccg gccaaactca ttgctacccc caaatttgaa agaaaaatgc cggcattcct
    36121 tgggcaggaa caggtgcgtg atgtatttga ttatgtcgat gccggtacac cggaaggctt
    36181 acgcgatcgc gcaatcctcg aattgtttta tggctgcggt atccgtctta gcgaattgac
    36241 aggtctcaat ttttccgata tcaattttac cggtggtacg ctctcggttt tcggtaaagg
    36301 cgccaaacag cggattattc ctatgggaaa acatgccgga gcggcattgc aaagttacat
    36361 tgattaccga cggtccgttt cgtccgccga taaaaacgcc gtatttctcg gaaaaaacgg
    36421 taaacggatt accccgcttg ccgttcagcg tttggtcaaa cgcgcactcg aaaaagtatc
    36481 cgatgccaaa aaactaagcc cgcatatttt gcggcattcg tttgccacac atttactgga
    36541 caatggcgcg gatctgcgtg ccgtgaaaga tttgctgggt cacgaaaatc tttcaaccac
    36601 gcaggtatat acgcacgtca cggttgatcg tttgaaaaaa gtctatgaaa aagcgcatcc
    36661 gcgcgcaaaa tgaaatgaaa atgttttgtt ataacgggca tgatcttcga ttgtgcccgg
    36721 gcttctgttt cccgtcggtt aattatctaa tttgaaggag tctgttatga aaatccgtat
    36781 cacggcacgg caccagaaaa tgagccctaa agtgaaagcc tacatcgaag aaaaactgga
    36841 gaagctggat cggtatcatg atcatatcat tgactgcgaa gtcatcgtgg acaaagaaaa
    36901 actcaaagag gtcgtcgaag tcaacgtgaa agttttcggt aaagtgatca acgtcaaagc
    36961 caaagatgcc gatgtgacca aagcgaccga tttggccatg gacaaacttg agtcacagat
    37021 caaaaagttt aatgataaga tcaaagacaa aaaaaagaag tccgttaaaa aaatgccgat
    37081 catcgatgaa gatgaagaat aattttgaaa tcccttaata atccattaca ttggggctgt
    37141 tcacaaaaac agcccctttt ttataacgta atctatcaaa cataacgcat ccggcggtgc
    37201 ggcagacgag tactatggca aatctcaatc aaagttacga cttcatcaaa caaatcggtg
    37261 ttggcggcat ggcgaccgtt tttctcggac ggcatcctgc gttggagcgt ctcgtcgcga
    37321 tcaaagtcgt gaagggcgaa caaaaagata aagtgcgccg ctttgaacgc gaagcccgat
    37381 tgtcggcgac actcaaacaa gaaaatctcc cggcgatctt tgattatttt accgacgatc
    37441 agaataatca ttatttagtg atggagtacg ttgacggcgt tgatatcagc ccgatcatca
    37501 aatccaaaac gacgattcct ccgatgatcg tcgccatgat cgctcgcgaa gtgtgccgag
    37561 gcctggaaca catgcacgaa aacggaatta ttcatcgcga tatcaaaccc agcaatgttc
    37621 gcctcggaaa aaacgggcaa gtcaaactga tggatttcgg tattgccaaa catgaagaag
    37681 acgccggaaa aagtcacctg accgcaaccg gcgtgatcgt tggcacaccg tcatatatgt
    37741 cgccggaaca ggcctcgggc gatcagctca caccgcaaag tgatgtgtac agtctcggta
    37801 ttatgatgta tgaaatgctg accggtaaaa agccgtacac ggccgatacc aacatgaccc
    37861 tgatttcgct gatcgcgcag gggaaattta cgccgctatc cgagacacat ccgcacttgc
    37921 cgccggcatt ggtcgatatt gtgcaccggg ctatggtcaa aagcctccga caacgatatg
    37981 cgacgacgag cgagattatc aaagatctca acaaatatct tcaatctatg tcgcaaatag
    38041 aaattcgtga ttggttggtt cgttattacg aagcggcaac caccaaagat aaaattgtgg
    38101 acatgaatgt atttgttttg ccggctccgg tgtctgtgga aacagcggaa acctcaacac
    38161 gcccggtcga tacggaaaaa aacaataatt ttgttctgaa taagcgccac ctcgtcttgg
    38221 gcagcgccgc actggttgtc ttgtgtgttc tggcatatct tttttggccc agtgatcatc
    38281 cgctgggtaa ttatgatccg tatggccgtt tagcgctgag tgtacgctcc gatcgtcaga
    38341 cgatcaaaga tacccgtgtc tatgtcaatg attcggaatt tccattggat gataattttg
    38401 gcggatcgtt gattattgat aatttacaac ccggacgcaa cgcgattaaa attcgttttc
    38461 cgcttttgta ccacacgtat gaatataatt ttacactcga cggtgcggac gaatccaaag
    38521 ctctcaattt agatctgacc aaaatcgcag cttcgttgga cggtgttcgt ccggagtcgc
    38581 gtcgttacgg atttgccgtt atttctgaac cggccggcgt atcggtgcat cttgatgacc
    38641 tcaagtccaa accgctcggc aaatctccga cgggcttgat ttttccgtcg gtaaaaacgg
    38701 gtttgcataa aatattcctg caaaaagaag gttatgtgac gagcgccata gaacgtaact
    38761 ttacaccgga tcaaaattat acattgcaat acgaactggg accggccaag aaaaaatgat
    38821 cgaaacgacg gacataccga tcctgcgcaa aggcgacgat gtaacgcttg atatcgaaac
    38881 ggtcacgttt ggcggtcaag gcgtggcgcg ttatcatgat tttgtcgtgt ttgtggaggg
    38941 cgccattacc ggcgatcgcg tacaagcccg gataatgaaa aaaaagaaaa attttgccga
    39001 agcccgatcg ctgcaaatta tcgaaccttc acgttttcgc gtagatcacg cctgcacgca
    39061 ttacggaata tgtggcggat gcaaatggca ggatgtggat tatgatgcac agttggaatt
    39121 taaacgccgt aacgtgattg atgtttttga acgcatcggc ggttttaaag gactcgatat
    39181 acccgcaccg ctcggatcgc cgcgcatttt tcattaccgt aacaaaatgg agtttacgtt
    39241 cggcgatacg gagtggattg tagataaaac atcggaaacg tcgccgcgtt cgttcatgct
    39301 cggtatgcac gttcctcaac ggttcgataa aattcttcac attgatcagt gctatttgca
    39361 atcgcctttg gctaacgata ttctcgcctt tgtgcgcgat tttgcacagc gtagcggatt
    39421 gccgccgtac tccgtgcaga ccaacgaagg attctggcgt ttcttgatta ttcgtgagac
    39481 cgtacatacc ggccatttga tggtcaatgt tatgacgtat tcggatcagc cggaggttat
    39541 gaaggaattt aaacaggcga tcataaatag gtttccgcaa attaccagtc tgatcaacgg
    39601 gattaccgac cgcaaatcgc aagtagcgtt cgccgaacgc gaaaatcttt tgcacggaca
    39661 aagcgtgatc actgaaaaat tagacggcct tgaatttgaa atatcatcca gttcgttctt
    39721 tcagaccaat acattgggcg cagaggtgct atatcgcacc atccgtgaaa tggcggatct
    39781 gaaaggtcac gaagtagttc tggatttgta ttgcggtaca ggcagtatcg cgatatatat
    39841 tgccgaccgg gcgcgcgaag tgatcggtat tgaattaatc gaaagtgcga cccgcaatgc
    39901 ggaaaaaaac gcccaacgaa atcgcgccga caattgccgg tttattaacg gtgatatgcg
    39961 tacaacgctg gacacgttgc aaaccaaagc ggatgtggtg atactcgacc cgccgcgctc
    40021 cgggttacac gaagatgtca ttcgaagtgt gatgcgtttg gggccggata aaatagttta
    40081 cgttagttgc aatccttcta cgcaagcgcg cgacttggct ctgatgcaag cggattatac
    40141 catcgaacgt atacaaccgg tagatatgtt cccgcacaca taccatatag agaatatcgt
    40201 acaaatgaga aaaaaagtat gacgtccaaa cgccacaata tcctctgtgt tgatgacgaa
    40261 ccggtcatat taaatatcct gtcgaaactt tttgaagaaa agtatcaggt gtacacggcc
    40321 aaaggtgcgc tggaggcatt ggagctgctg cagcaacaca gtgtggcggt cgctatcgta
    40381 gatcagaaaa tgcccaaaat caccggcgtc gaattgctca aacgtataaa ggagcaatac
    40441 cccaatatcg tacgtatcgt acttacggcg tacacggaca tttccgattt ggtcgcttcg
    40501 atcaatgagg gcgaaatttt ccgttatatc accaagccgt gggatagtga agcgctcatg
    40561 tgggatgtgc atgcggcgat cgaaaaatac gaagaaaatc ttgccaatgc ccagcgtatt
    40621 gatgatatca cccggcttca gttggaaaaa gaaaagcttc agaaggaaat cattcttctc
    40681 aagtccgaga ttgaaaaaga atatgctctg gataatatcg tcagtgtcag tccgagtatg
    40741 aatcagattc ggcgtatcat caaggctgca tcacttgccg atgagaccgt acttattacc
    40801 ggcgaaagtg gcacgggcaa agagcttgtt gcgcgggcca ttcatcataa cagccaacgt
    40861 cagaattttc ccatggtagc ggtggattgc ggtgcgttgt ccgaatcgct gctggagagc
    40921 gaactgttcg gtcacgtcaa aggcgctttt acgggcgccg tagcggatcg taaaggactt
    40981 tttgaagaag ccgacggcgg gacgattttt ttggatgaaa tatcaaacac cagtttgcct
    41041 ctgcaagcac gtctgttgcg ggttttgcag gagcgggaga tccggcgtat cggcgatacc
    41101 aaatcacgcc cggtcaatgt gcgcgttatt gccgccagta atcgggatct cgtcgaagaa
    41161 tgcaaaacag aacgttttcg cagtgatttg ttttaccgtc tcaatgtgct tccgatcgct
    41221 ttgccgccct tacgtgatcg ccgcgacgat attccgttat tgatcaatta ttttatcaat
    41281 gagtacaata aaaaatcggc caaatccatt gtttcgattt cccgcgaggc gatggatcat
    41341 ttgtcgtctt tagaatggaa aggcaatatc cgcgaattga aaaatatcat tcatcgcatg
    41401 atggtgtttg cggagggcag tcatttggag atgggcgaca ttcccgacga attgctcaaa
    41461 gtcaaacggt cggcggtggt gcctgttttg cccgaaggga gtacggtttt cggtgtgagt
    41521 gcgctgcttc cgatcgaaga tatggaacgg gagtatatac ggtttgtgtt gcatcaaaca
    41581 ggcgaaaata aagccgaagc gtctaaatta ctcggtatga agcgtacgac acttgtgatg
    41641 cgaatgaaaa aacttggact tttagcataa cctttgacgc ggtttcaata catacttttt
    41701 tataaaccgt acggtgttat ttgccagttt tcatcgcatg aaaaacatcc ttccttagca
    41761 gaattcaaat ttccgaaaaa tgtatacccg gtcggtcgtt tggacacgga cagcgagggg
    41821 ttgttgatcc tcaccgatga cggtgatttt cagcatcgct tgatcgaacc gcgttacggc
    41881 catgcgcgca cgtatcatgc gcaggtggag ggaattcccg atgacggagc gttggataaa
    41941 atgcggcacg gaattctatt ggacagtaaa cccacattgc cggcacgggt gacggtattg
    42001 gaaccgccac cggatcatcc gcttcgcaat ccgcctatcc gctacagaaa aaatattccg
    42061 acgacgtggg ttgagttggt actcaccgaa ggtcgaaacc gccaagtgcg acggatgaca
    42121 gcggcggctg gttttccgac gcttcgctta ttgcgcgtgg gcatcatgag cttgtcattg
    42181 gagggattac aacccggttc gtggcgagcg atgacgactg aagaatttcg ctcatgtgtg
    42241 gcggttgtaa aaaaataaag ccggattgct tttacgcccc acgtgaatta tcttagcaaa
    42301 gaaagggagt tctatgcatt tttcacaatc cgaactgctt atcaatattc tttttttgct
    42361 ttttatcatc gtattggcca tccagtcata catcctgtat cgtgtgcgtc gttcgcttac
    42421 gcaagcaagc caattcattc agctcatgaa tgtatttttt aaagagattt ccaaaacaca
    42481 ctccaaaagt gcatcattgg cggtgaacaa tcaggatgtg atgcggaagt tgcgaagtaa
    42541 aaccattcgt tccaacccct cgcacgacgt ttcgtgccag gcgtgtaaat acagattatc
    42601 gtatgtcagt ttggatcgcc cggaaatgga ttttacctac gcttgccagt tgactcgcgc
    42661 cacggtggta ttggatgatc aatgtgaaaa atttgaaccg gatgctgaat ccgcacagcc
    42721 gtcgaataaa taaaaatatt ctcaaaagtt tgtatcgttt catcctcttc cacggcacat
    42781 ttcatatctc attttttctc acagcaaaca gatatcatag ataggacagg ctcggcatag
    42841 aacgatccgc cgtttgcgtg ggcacatttt gagaatgccg agacgacaaa tcgcaaagtc
    42901 gtattttaca ggatctatcg cgtctaattt ttttaacgca taggtaatct ccatcgccgt
    42961 tttttgtgaa gtgtctgtac gtttggttaa accgatataa aatgcaattc gctgaatatg
    43021 ggtatctacg ggaatgagta atttcgacgt cggtagattc cataatccta aatcaatacc
    43081 atccttacgc accatccatc gcaacatcaa aaacgcgcgt ttacaggcgc tttttttgga
    43141 tggatcagcc aaaagaaatg taaaaccaac gccgcgtgca tgccgccatt gccaatgttt
    43201 tttatcggtg tctttcaatt catttacaca tcggctccat gtaatgccta gatgcacatc
    43261 ttcgtttcga tagtgtgact caatccattt tccgagcgtt tgatgttttt gtaaaatgat
    43321 tttcaaagcg tgtaggacac gcaaaacatc ttctgcccgg atcatacgat agtaccattt
    43381 ttgccaacgc ggtaattcat gctccggatc gaaatgggtc acataataat gcggctgatt
    43441 attcataagt gtcagcatag aggataacgc cttgttgatt tgcgctacct gaccgaacgc
    43501 catggcggat gccaatacag ccatgatttc ctgatcacac ggttgttcga aacagtgtat
    43561 catttgaatc ggatcgctgg cgatatacga cgcatcaaac gtattataaa ggttatccaa
    43621 aagctgtttt cgtttacgca gcgtaacgtt gcggggtcga atcacagagg tgtcctttgt
    43681 gaaggagata ggttgtctaa tgttgtcttc caatgtataa acgaaaaaca tccataatgc
    43741 caattggtat ttagtattaa aaaaaagcct tattctcaac tatgatttga aaggttcgaa
    43801 taccggtttt ttaattttct tgcacaggct cttgagatta gttaagtttt aaacgcttgc
    43861 ctgttgttct cacttcttaa tgttcgctac gtttcgcgca tggcgatgac atgccgatgg
    43921 ttatgaaagt aatgactatg atcaaaaaac gaattttggt cgcttggttg ttttttatcg
    43981 gaactacctt tcttttttca caatcccgtg aatttgaatt taaacgtcta accgtagaaa
    44041 acggcctgtc gcaaagtaat gtgtatgcca ttgtacaaga ctatcgcgga tttatttggt
    44101 ttggaacgca agatggcctc aaccgttacg acgggtatca gtttagcgtt tttaagcatg
    44161 atatagatga tagtaattcg atatctatga attggatcaa tacgatttac gaagataagc
    44221 atcgtgtgtt atgggtcggt acgcaaggcg gcgggctttc gcgcatgaac caaaccacag
    44281 agcattttcg taattttacc gctgatggac aggccgggtc gctaccgtcc aatgacgttt
    44341 atgcgatact agaagatcat atcggcaatt ttatgattgc taccggccgg ggcatcgtgt
    44401 acatggatcg tgaaaaggaa acttttcgtc ctgcgtctga aattccggaa ttaaaatatg
    44461 ggcaaataag agcattgtgc gtagaacgct cgggcactgt atgggccgga acacaacttg
    44521 gcggtttatt tgtacgcgat cgtcagggcc agtggtttcg catttcgatg caagtcgctg
    44581 gtcgtccgca aagtgatgaa ttggccgttt cctcgataac cgaagatgtc actggtcaag
    44641 tttggatcgg tacggacaaa ggattgttca attgggacaa cgggacaatg agcaaataca
    44701 cttcaccgaa gttggaaaat aaagttctga cggacgggcc gataaaccat gtatccacgg
    44761 atcgtgccgg gcgcgtttgg ataggcacag ataacttcgg tcttttcgga atatttgata
    44821 cgacacgatc ggaagctgta cgttttatca atgatcagct caatccgcgc agcttaagtg
    44881 aaaatcgagt ttattcgact cttgaagatg atgcgggcaa tgtttggatt ggtacgggcg
    44941 gcggagtgag tatttggaat gcccgtgcga ataaatttcg tgtatatcgc cgttcgccga
    45001 cttctcaaaa tacgctgagt cacaatcacg tttgggatat tttggaagat cgtatgggtt
    45061 ttttatgggt aggcactaat gacggcctca atcgtttaga cagaaaaacc ggtaactgga
    45121 aaaactggcg ttataataaa acacatgcat ccggattaag cgctgaacgc gtttggacga
    45181 tttatgaaac acgtgacggc gaaatttggc ttggtatgtc cggcggcggt ttgtcgcgtt
    45241 acgatgcggc aacggatcgt tttatacact ttctccatga tcaaaaaaac ccagcatcga
    45301 tcaataacaa tcgcgtgcgt agtatagctg aagatcatga tggtattctg tgggtcgcta
    45361 cacgtggtgg cggactcaac cgattggatc gtaaaaaaaa tgttttcaca tcatatcaat
    45421 acgatggtcc ggacggaaaa ggtatcagtt ccaatgatac gtatgttctg cttgtgcagg
    45481 atagcgtttt atggataggt acgtttgaca atgggttgaa tagccttaac ctgcgaacga
    45541 tgacgttcac gcgttatacg cacgatcctc gtaatccgtc ttcgattagt tccaataaaa
    45601 tttcttcgtt gtgtttggat cgtaatggcg cattgtggat cggtacaacc gaaggattaa
    45661 accgtttgaa ccatcaaaga aacagttttg aaagatacac acctaaagac ggtttaccca
    45721 acgaagtcat attgggagtt ttagaagata ataaaggttt cctttggctt tcgacgaatc
    45781 gcgggcttgc acggtttgat ccgcttgccc cacgcggcca aaaattcaga aactataatc
    45841 cgaatgacgg attacaggct gacgaattta acggttcatc gtttcataaa agtaaaagcg
    45901 gtgaattgtt ttttggtggt gtcaacggat tcaatgcgtt ttttcctgaa gaaatcaaag
    45961 ataatacgca tgtgccgtcg gtcgtactga cggcgtttaa aatttttgat aaaccgtttc
    46021 gtcttcaagg tgcgatagcc gctatggatc aagtgacctt gggtcaaaaa gaaaatgcat
    46081 tctcgttcga atttgccgct ttagatttta cggcgcctac aaaaaatcaa tacgcgtaca
    46141 aaatggaagg gtttgatgaa gactggattt actgcggaac acgccggtat gcgggttata
    46201 ctaatctgga tggtggcaca tacacatttc gtgtcaaagc gtctaataac gatgggattt
    46261 ggaataatga aggtatcgcc gtgcaggtta cagtggttcc tgttttttat aaaacggtat
    46321 ggtttcgcat ccttgcatca ttggtcatag ttcttgtggc gtattccttc taccgttcgc
    46381 gcctcaatcg cgtaaatcgg atcaaagagt tgcttgaaag aaaagtggcc gagcgtacaa
    46441 aagacctcag cgagatgaat gccaaaatca tggaaaccga tcgcctaaaa tcagaatttt
    46501 tggcaaatat gagtcatgaa ttgcgaacgc cgttgaatgc tattattgga ttttcagaat
    46561 tgcttattga cgaaatgcgg cattcggcga atgcggatca actccaaaat ctcagtgaca
    46621 ttcatgacag cggtcgccac ttgttgatgc taatcaacga tatacttgat ttatccaaaa
    46681 tcgaagccgg gaaaatggag ctttttccac aacagtttat tatggaggag ttgttggaat
    46741 cggttcacca tacaatggct ccgctattga atagaaaaaa tcaaacgctg tcatggcagc
    46801 tagataccaa tgcgccggcg gtgtacgccg atcaaaacaa gatcaagcag gttctgatta
    46861 atttattgtc caatgcgatc aagttttctc cacacgacag tgcgatcatc gtcacatcaa
    46921 aagcgttgaa taccgatgag aacgatatgg tggaaatagc cgtacatgat cacggaaaag
    46981 gaattgcgcc ggaaaatctg gatttgattt ttgaagagtt tcggcagatt gatggaagcc
    47041 atgcccgtgc ggagcagggc acggggcttg gtttggcact atgcaggcga ttgatcgaaa
    47101 tgcatggcgg taaaatcagc gtcgaaagcg aaatccatca cggtagcaca tttcgtttta
    47161 ccatcccgcg cgccgttgca ggatcttcgc aagaagttat gtcggaaacg ccggtggatc
    47221 ttacacatca gtcgttgatt ttgatgatcg aagatgatcc gcagtcggcc aatctgctcc
    47281 gacgctatct tgaaatagcc ggttatcgcg ttgcgcatgt gcgcaacggc tcggaagctc
    47341 tggaagaagc gcggcgactc aagccgatgg cgattacact ggacattatg ttgcccggta
    47401 aagacggctg gcagatactg caggaattaa agaatgatgc cgatacgtcg gatattccgg
    47461 tatttatcgt gtcggtcgca gagaataaag agttggccta tagtcttaaa gcggaagatt
    47521 attttaccaa gccgattgat cgcgaacgct tgatccatcg tattggccag ttgcgtatgc
    47581 gtcgtatcac gggcgaaatt cgtcagatac tcatcgtgga tgacgatccg aatgcattga
    47641 gcatacttga aagttttttg tataaaaacg ggttccgtgt tttgcgtgca cacggtggca
    47701 gagaggcgct tgttatgatg cacggcgcga cgattgatct ggtgctgctg gatctgatga
    47761 tgccgggaat gtccggtttt gaagtagtag aagccatgcg taatgaagaa aagctgcgcg
    47821 aaataccgat tattattttg acagccaaag acctgacggc cgaagaacgc gagcggctgc
    47881 gtatgcatgt acgtgcattg atgcaaaaag caacatacac aacggaagat ttaatagaag
    47941 aaattcgaag aatccacccg aaaacaggag cgctgagtaa ttcatgataa agcaaaaaac
    48001 aatactgctg gtggaagaca atgaagtcaa tatgcggctt gtggaacgta cgatcaaaaa
    48061 tccggcttat acgatcgtaa gcgccggtaa cggtgaagaa gccttggagc gtgctgcaca
    48121 gtacaaaccg gatttgatta ttttggatat tcaaattcct aaaatggacg gttatgcagt
    48181 cgcgcgaact ctcaaagccg atccggcgtt aaaacatatt cccattattg cgttgacggc
    48241 gcacgcgatg aaaggggatg aggaaaaaat cttagcggca gggtgtgaca gctacgtcag
    48301 caagccaatc gaggtgtcaa aatttcgcga aaaaattaac gacctcttgt ccgataaatc
    48361 ctaatagttt taatagatac tacaaaatta aatattataa atacgtgtta cttgaaagat
    48421 aattctgcac atagtctttg accgactcct caagtgatcg aaatcgaatc ggacaacccg
    48481 cttcttgtag ttttgtcatg gttgcttccg tatggtattg atatgcattg cgaataatct
    48541 ccggcatctc aaaatactca atcaatactt gtttgttcat agcatgaaac actgcctttg
    48601 cgagatcatt ccacgaacgt gcctttcctg tcccaagatt aaaaattcca cctacatcac
    48661 ggtgttctaa gagccaagcc atgatatcta ccgtatcttt gatgtataca aaatcgcgga
    48721 cttgagcgcc atccgcgtaa ttcggccgat gcgatttaaa gagtcgaatt ttcccgcttt
    48781 ctaaaacttg ataaaaagct ttgtttacga ggctacgcat atcacctttg tgatattcgt
    48841 ttggaccaaa cacgttaaaa aactttaggc cggctacacg ggattgtaaa cgttcttgta
    48901 aaatccaaag atcaaaaata tgcttggaat aaccgtattt attcagaggt ttgagcgcgg
    48961 acatttgtgc atcgtcatcg gtaaaaccta attcgccgcc gccgtaggta gccgcgctgc
    49021 tggcatagat aaaacgtatg ttgcgtgcca atgcccaacg tgctaagcgt ttggagtagt
    49081 gatagttatt tcgcataaga taatcggcat ccagttccgt cgtggccgag caggcgccca
    49141 tatggataac cgctgtcgta tcatcaaaga atgctttatt atccagcaaa cgcaggaatt
    49201 catctttgtg aatataatcc gtaaaacgtt ttccgataag gtttttccat ttatcgctgg
    49261 ttcccagatt atccaccacg atgatcttat catgccccag atcgttgagc ttacgtaaca
    49321 tggcgctacc gataaaaccc gcgccgccgg tgagaaggat cataaaaaaa ttcctctgaa
    49381 aattttcagg ggaatatcta aaattagcaa tgcattatca acggtaatac tatagcagat
    49441 taataccgga tgagacgcct gtttttgatg gaaatttgta tgtgcggttt aaccgcaagc
    49501 gtaaaaccct ccggttacca ccacgaggat gagtaaccac aggattaatt ccctgaattc
    49561 ctttttcata cggcccctaa ttactttaca taagtgtacg atacatacac ggtcgaaaga
    49621 gaaagtaaat cttctaattt tcagcgcgaa tcacttcagc aataaaacta ccgcaggctt
    49681 ttgttccttc gctaccgccc agttctttgg tcagtttgtt tttttgtatg gcgacgcgta
    49741 cagcgcggtc gatcatatcg gcctgagcag taaatccgag atggcgaagc atcatttcgg
    49801 ctgtcagaat ggaagccaca gggttggcga tatctttacc ggcaatgtcc ggtgcgctac
    49861 cgtgtacggg ttcgaacatg gaaacgccat tcggattaat attacccgaa gctgcaaggc
    49921 ccatgccgcc ttgaagctgc gctccgatat ccgtaatgat gtcgccgaat aaattattag
    49981 taacaatgac ttcaaacatt tccggttcac ggatcatcat catggcgagt gcatcaatat
    50041 aaaaatgttt ggcctgaatt tgcgggtatt ctttggccac ttcaaagaag caacgatacc
    50101 aaaggttatg accatatgtc attgcattgc ttttgtccgc catggcgact tttttatgtc
    50161 cgtttttagc ggcgaattca aacgcgtaac ggataatgcg ctccacgcct ttgcgtgtat
    50221 tgacttcctc ttgaatggca atttcatcgg gagtattttt cttgaaaata ccgccgacac
    50281 tgacatagag accttccgtg ttttctcgga aaaccgtaaa attgacatcg tgaacggttt
    50341 tatttttaag ggggcaaaac cgctcatcca acaatttgac aggacggaaa ttgacataga
    50401 gatccagttt aaaacgcgta ccgagaagaa tatcttttgc gtgctcgtta ccgggaacac
    50461 gcgggtcgcc gaacgcgccc atgagaatgg catggtagtt attccggaaa tcatcgtatt
    50521 gctcttccgg catcgttacc ttggttttca gatacttttc ggcgccataa tccatcgtgg
    50581 tcaactggat atccatcggc ttacgcgttt ggaccgcttc aatgactttt agtgcttcat
    50641 ttattacatc agttcctatc ccgtctccgg gaattacggc tatgcgcttc attttgttaa
    50701 tccaaatatt gatatttttt tagcgcgcaa aagtaagcaa tacacccaaa aatagcaagc
    50761 aaaggcctat atctaattct accgatagag ttgcttgcca attgctcaat gggaagttat
    50821 ttttgaaaga gagcaatcaa caaccaagca aatataggaa ttgaatcatg tctatatcgc
    50881 tgcaggaata ttttcatgcc atcgctgaag ctacttacgg aaagatcggt gatgattttt
    50941 ttaaatcctt ggtccgcaac ctcagttccg ttttgaaaat ggagtttgca atggttacag
    51001 aatgtgcaaa ctcagaaaaa agcagactgc gtaccattgc acattggatc ggaatggatt
    51061 tcggaaaaaa ttttgaatat gatatcaccc atacaccttg tcacatggtc attcatggcg
    51121 gattgcagtg ttatacagag gatttgccgg ctcaatttcc tctcgaaaaa gagctttctg
    51181 aaatacaagc gacgagttat atcggcattc ctttgaaaaa atcaaacgga gaaatagtcg
    51241 gccatctggc gcttttggat acacgcccta ttttagatgc ggaaattaaa gccagtattt
    51301 tgaaaatttt tgcatcccgt gccgaagccg aattagaacg cttacaggat aaacgtgaaa
    51361 gagagctatt gatttacgaa ttgcaggaaa gcctcgaact tgttcaaact cttcgaggca
    51421 tgttaccgat ttgtgcgtcg tgcaagaaaa ttcgtgatga taaaggttac tggaatagga
    51481 tcgaagaata tgtagagcgg cattcgggcg cgcaatttac acacggtatt tgccctgaat
    51541 gctcagtcaa actctaccct gacctttaac gaaggtttgt ggcgtattta atgcttttta
    51601 tgtccccaca ttttttcgcc ggcttttatc ggtatcggca gtttgttttg tagaggcggt
    51661 aacggacagg tagcgtaatc cgtaaatacg cagggcgggt tataggcttt attaaaatct
    51721 atgatgaccg aatcaccttg agccggcatt ttggtgtaaa gaaaacgacc gccaccgtaa
    51781 gtttccgatc cgttggtgcg gtcaccgaaa ataatgaata agtcatccga accatcttcc
    51841 aataccggtt cgagtgtata ggtttgatca tctatggtaa acgtcaaaag cccggggcat
    51901 ttcatcgaat ccacggttcc caaaatggta ggtattagaa ttattttcat cggatcgtat
    51961 tttttaattg tggcatgcgt ttgccagttc ttatcaacgg tgtaacgatc aatgccttga
    52021 aacgatttta gcaaagggct ttctaaatct ttaagacgaa tggcgtattc accatttcgt
    52081 ttgatcacgt aaaaacgaaa ggaacccatc gccaggcgcg tcattagtga gtcagagtcg
    52141 tctttcaaaa cggaagtaga aattgttttg ccgtcagcgg ttatgacgac atcttttttt
    52201 acagttaatg tgataccacc tttttgaaga gtaattgttc cggcaaacgg aggcgcctgc
    52261 ggaggaaaaa taagctgatt ggaagaatcg cttccgaatg tattatcgcc ctctttgagc
    52321 caatgcaatc cgactaagtt gagccatcct ttttcttttt tcagattggc ttcccgttgt
    52381 gcgtgccatg cgttgatctc ctgtacgtaa gcggcatcgg ttatgatcgg ttctttcttt
    52441 ttttggcagg cgcatatgaa aagaagcagt atggacaatc cggtaaagag aaaaaactta
    52501 cctcgtttca taagcgacat cctttttgag ttggcgtccg agtgcgatgg cgttttcgat
    52561 gttgcgatcc aatatgttaa agtgccccat ttttcgtccc ggccgggctt cagattttcc
    52621 gtacaaatga agttttacgt gcgtttgtgt gaaaagtttt tcccagtgcg gttgctgatt
    52681 tccccaaagg tcaccgagaa ggtttaccat cacaacagga gataataaag aaggatcgcc
    52741 tgccggtaaa ccgcaaagta cgcgtacttg ctgttcgaat tgatccgtaa tgcacgcatc
    52801 cagcgtataa tgtgcgctgt tatgaggacg gggcgccatt tcgttgacca atacgcgccc
    52861 atccggcatg acaaacattt caacggcaag cacgccatga tagtccaatt tttcagcgac
    52921 ctgtcgcgcg atggtgcgtg cctgactatc cattgtcccc gcgatacgcg ccggcacgat
    52981 tgtcatatct aatattccgt tttcgtgtat gttttcgccg gtgggatatt ctgtgatgct
    53041 tccatccacc gcgcgggcta gtacaacgga cacttctttt tcaaaattga gcatttcctc
    53101 aagaatgcac ggtgcgtcat gcatggcacg aaaagcatta cggacatctt ggaccgtacg
    53161 aacacggtat tgccctttgc cgtcataacc gtaacgatta cgtttgagaa ttgccggcag
    53221 tccgacaaag tgtacggctt catctatatc ggactctgtg cgtatcgcag caaatttgtt
    53281 cgtcgggatt tgatgaatac gtaaaaatga tttctcgtgc aatcggtctt gcgtaacgga
    53341 aacggcttta gcggatgggc gaataaagca gtgatgcgat aatatttcaa gtgtgtcggc
    53401 gggtacgttt tcaaattccg tagtaatggc agagcactcc gtacgtaaaa tatgaagcgc
    53461 ttcagcctcg tgataatcgg ccgtgagatg gcgatctgcg atatgtcccg ccggactctg
    53521 cggatccggg tcaagcacga tgacgcgata tcccatcgca tgagcggcca tagtaaacat
    53581 gcgtccaagc tggccgccgc cgagcatacc aagtgtagag ccgggtttaa tcatgcatta
    53641 taaatcttaa gaagttttta aatggatgcg tactgcaatt attttggcaa cttgagttgt
    53701 aaaacttttc gagtctggga ttcgcgaaaa gcagaaagtt ttttcgctaa agtgtcgtcc
    53761 gtcgttgcca agatggctat ggcctgtaaa gccgcatttg cggccccgga ttcaccgatc
    53821 gcaaacgtag ccacgggaat acctctgggc atctgtacga tcgaaagaag cgaatccagt
    53881 ccttttaagt atcgcgaagg tacgggcacc cctaaaaccg ggacaatagt tttggaagct
    53941 aacatccccg gtaaatgagc cgctccgccg gctccggcaa taatacaccg caatccccgc
    54001 tcttgagcgg aggccgcata ggaaaaaaga agttccggtg tacgatgggc tgaaacaaca
    54061 cgcgcttcat aagggatttt gaaagtcttt aactgttcta ccgcatattt catcacttcc
    54121 cagtcgctgt cgctccccat aaccacgcca ataagaattt tttgagccat gatattgtgt
    54181 ttctgatatt aaacttgtgt tgatgatacg agtttcatgc ataaccctca agttttaaag
    54241 caatttcccc tgattgtaat atgtcaatgt acaggaaact catttaacaa aatttcgtaa
    54301 aaataattac gtaagacaat acattttatt ttggaaaggt tcgttttatg tttaaaaggg
    54361 taattgaaac gtcagatgat tcgatcctga cgattttgcg cgtagtttcg gggtcggtta
    54421 ttctgatgca cggcttacaa aaacttttgg gttggtttgg cgggcatggt gtgacagcta
    54481 caatgcaagc gtttgaagca tggtttggta tgccgtacat cgtaactttg ttagttattt
    54541 tatccgattc gttaggtgcg atatgcctta ttgccggcgc tgtaacacgt ttcatggccg
    54601 cgtccatttt atcggtaatg atcggagcaa tcatactggt gcacgggcgc tgggggtttt
    54661 atatgaattg gtatagtgaa aagcgcgggg aagggtttga atttcactta ttagtcatcg
    54721 ctataatgat cgtactcatt atgcgtggcg gcggtttatg gtcggtagat cggtggttgg
    54781 tgaaaaaatt taaagcgacc gataatatct aacagttatt tgcaataaaa aaagcagagc
    54841 cagtaagctc tgcttttata gaagagttga tatcacacgc agttttagaa tgcatgaatg
    54901 agcgataatc gtaattcacg ggagaactta ccgatatctt tactattatt gagaatatca
    54961 gtcattataa attcgaccat tactttttga ccaaaattga gtttagcgcc gacattcata
    55021 taacctcgtc cgtgcccgat aatgtcttta ttgtcattga gcgcgaggtc gtattcccaa
    55081 acggctgtta actcgtcatt gatgctttta tcaacaccga caaaaaatgc aggttgattg
    55141 tctttatctt tcttttcagt gaccgaccat gaaatgccgc cgtgaattcc taaatttccg
    55201 gcaaaggcat aatttttact ggccgtgata aaaagaccac gcgacttttg cgtgtaacgg
    55261 tttactttga ctgtatcaac cgatttgtaa taaggaccga aaccttggtt gttaaagcca
    55321 agtgttaccg cggggccgat cgttgattcg tcaacggcac gatatcgtac atcaacacct
    55381 gggagtgggt taaagttaac cttgccggag ccgataattt tttctccacc atacgaaata
    55441 ccgatcatga aacgatccgt tatgcctacg cctatattag aaagcacgcc accattagcg
    55501 aaaagtcgca ttccgatttc gtaattgcct ttgttgagca tgcctgcggt cggtacgtta
    55561 acaagctgcc gatgctgata aggcgtagcc gggttcgatt cttgagcaaa agcagcagat
    55621 acagcacata tgaagagcaa aataattttt ttcaagtgag ttcctccgtt tctgttttgt
    55681 gtacattcgt gcaaatgttt atacgtaaat gtacaaaaaa gttttaatca cgttttcgtt
    55741 tttggcgatt caaaaaccaa cgctcaccac cggtaaaaga taatgtcaag cgataaacgc
    55801 ggtcacggac tttattatcc gcgatttgtc cgcgctgccc tgcctccaag ctcaaatcaa
    55861 aactgcttcg ttcgcggtta aacggcatcc caatacccaa agtaaacatt atttcgctgg
    55921 cgtgatttcc tgtcgtattg gttgcataca aattgttaat cattaggccg aagcggtaat
    55981 aattcttatc ccaccagggg gcagaaaagg cgttcgatgg ttgcctttcg ataccgctgg
    56041 accaacgaac ggtgtttcta tttttttgtg tgggactaaa ttcttttacc gatccccatg
    56101 ttccgtaaac gatatccgtc gccaataaat aacgcttagt ccatcgataa gacgcgccaa
    56161 atccgatttc tgtagggtaa ttcaaatttt gttcatagga tacagtcgta tcgcggtaag
    56221 taccgaccac acgtatatcg ctggatattt ttccggccat cgacagaaat ccgccaaaat
    56281 tccattgctc attatgttga taaaggcctc cgacgaccaa tcgaccgccg ttcatgtttt
    56341 gatgtcttac gtcgcgcgta tcaactaatt catccgagct atagttaatg tcccattctc
    56401 tttttatggt gccgaagaga tactgcaacg atgcgcctat ggtccacttg ggattcagtt
    56461 tgcctgctcc ggtaaaggca agttgttgaa gcccacctgt tccgattaaa gtttcggaat
    56521 atttttcacc ttcaaagatc aaattcggtg ttgaaaattc gtaattgaca tttgttacgc
    56581 gcgagagatt aatcgctgcg actaacgtct ttccaaaggg aaacgcaaat gccacatggt
    56641 tgatcgtagc gcggttggag aaatttttgg aagcgccgtt actggcttga ttccattcgg
    56701 caaacatgtt tagctcaaaa cgtgtcatcg gaatcccgag taacgtggcg gggttttgca
    56761 tgttaagcgt atagtaactt ggaatggcta aacctgctcc gcccatgccg agcgatctgg
    56821 ggttcggttt gtattcgtat agtccataag tggtcgcaga atatgaagaa ccgcctgcat
    56881 aagcagtcga agttgccaaa tttgcaaaaa tcattaatgt tagaatagcg gtatatcgca
    56941 taatgggctt cttttattgc ggtgactgag tatagtaaat gattattttt ggagaattat
    57001 cgcgatcgga cggattatta taaaaacgga cgcgatataa tgggttaaaa ttaccgttga
    57061 atatgttata gttcatcaat accaagccgt tattgtggtt tggttgggtt gcccaatctt
    57121 gaacaaacga ggaaatattc agttttaatg tgtcatcgcg aatattgtcg gtcctcgaat
    57181 tcgttgtggt ttgctcaata taccccggtt tccataagct ggtatctacg gtggaagcca
    57241 tagcttgaat atttatcgaa tcgctgctat tacggaaata atttccagaa gcctcagttt
    57301 tcagtactaa tacggcggag tttatcgtta aatccgatgg aagcttatct gtattaaagc
    57361 gaatcaaaga acggtatggt acgcctccgc caacagtaat ggaattcaat gcttgcgggt
    57421 tgcgatcacg ataaacgtat ccgtggtagc cgccgttata aacaccggaa ataaaaacgg
    57481 gagagacata aaaagtacta cggtagtttt tatcgtcagg gtcctcgtaa tccccatcac
    57541 catttttatc gcgcaaatac acatactcga cttctattcg gggtaccaga tcaaaatcaa
    57601 tgattgttcc attgattgta ataatggtat tgttcccact gtaaaattgt tgcgtaaaag
    57661 gggctccgcc atcaaaatca atgatgatgc cgttatttgt tttagggttt atcgtactgg
    57721 tgtcgtttct tgctttgacc cattgtacta cactatcttt attaagtgtt atttccaacg
    57781 aatctttgtc atttaatttt gagaagttaa tcgttccgat tgagttgggg ctataatctg
    57841 taccttgttg aaatttatgc cataataaat ccagttcact ccaaggtgtt tgaacggtgt
    57901 ggatgtgcgc attgatacta ccagcagagc cgaatgcatc ggaagcgacg agttgaaggc
    57961 gtgttttgac aattatcaaa ctgtcaaggt tgatgagttc tccgctggga tccgtaatgt
    58021 agtcagatgg gaaagcaaaa cgcattagaa ttcgaaaatc ataattatct actgaaccca
    58081 aatacaagcg agaggagtta taagtcgaaa tcgaatcaac aaatgaagca atccgttcga
    58141 tatcatcggc atataatgta tctgttttga aagttccggt agaggtaccg cggtcaataa
    58201 cttccatgcc gacgtctttt aggtggtcat cgcatgatat aagagaaacg agcagaaata
    58261 aaaagccatg aaagatgttt tttgaaaacg aagcgataaa gaaactgcgc aaaatgaaat
    58321 ttctcccgta ctttaaaatt taatgaaaat gcggtgaccg cagaatttac aattggatcc
    58381 gcgcataccc ccgagattgg attggtaatc gcggcgtttg atgatcagac gtttacagtt
    58441 gggacaaaat gtatccgaga caccgtcaat gtccgtatcc gtcaaatata cataactcag
    58501 cttttgtgtg gccagtttgt atgttcgcac catcgtttca ttgggtgtca tgggttgcga
    58561 cgtcatttta tggtgcggga aaaaacgttt aaaatgtacg ggtatgtctt tattgatgcc
    58621 cgcaacgaaa tcaacaaaat tacccacatc ttccgtactg tcggattcac cggggatcaa
    58681 caagtacgtc aattccaaat gcgtttttcc gaatgcggat ttgatggaat tgagtacgat
    58741 ttcaagttta ctttccgtta atcgttcatg tgtttccgcg ttcatagatt tgacgctgat
    58801 gttcatcgca tcaatgtaag gaataatgtc ttcaataggt ttttgattca aatatccgtt
    58861 ggtaatcaaa atatttttct gtccgatacc tttcagctta cgcatagagt caatcagaaa
    58921 ttcataccat atcaaaggtt ccgtaaacgt ataagccacg ccgatggaat ggctttttcc
    58981 ggaataatcc agaatcgttt gcagagatat atcttttgtc tcttttttat tcaatgccac
    59041 atcgtggttt tggcagaatt tgcaacgcat gttgcagccg ttaggtgcca acgataaaat
    59101 ggtggaattg gggtaataat ggaaaaatgg ttttttctcc atcggatcgt aaacggccgt
    59161 aacgatttta ccgtaattaa aagagtacaa tttgccgtca atattcttat atccccagca
    59221 gtcgccctcc gccccctcct gtattttaca catacgaggg catagttcac agacgatatg
    59281 atcggcttct ttacggtaaa atttcgcttc gatcatgata tagttccgct tcaatccatt
    59341 aagtaagtac tttgaaaaac aatatattta cactaaatta aaaatgcaat ttttaatcct
    59401 attgttttta taaggcttgt acgtgtatcg acggttagat gttcgtgctc aaaaaaagtt
    59461 taatgcctga ttttaatcga ataacgtatt tttttcctcc aagctaacgc ttgatgcatg
    59521 gaaacttaag ttaacggtag atagttatgt cctaccaatt cgaacatctc acaccacaaa
    59581 cgtggaatga ttttgccgcg ctgtttggtg aacgcggcgc atgtggagga tgctggtgta
    59641 tggcatggcg ccggtctaac gccgagtttc aaaaaaataa aggcgaaggc aatcgcaaag
    59701 ccatgcagcg attggtaaaa aaagaattac ctatcggttt gatcatgtat aaagatgtta
    59761 ttccggtggg atggtgcgca tttgccccgc gggaggaata tatacggctt gcggcttcac
    59821 gcattttcaa acccgtcgat gaaaaacccg tgtggtctat ttcatgtttt tttattcaca
    59881 aagattatcg taaacaaggt ttgtcggaaa agctcatccg gcaagtcatc gtatcggcta
    59941 aaaaaatggg tgttcaaatt ttagaagcgt atcccagcga cccgtatgat gctcaagtgc
    60001 ctgcgccatt tgtatggaca gggctgactt cggcttttgt caaagccggg ttcaaagaag
    60061 ttgcacgcag gtccaaaacg cgccctatca tgcgctacgt tgtaaatacg accgacttaa
    60121 ataaataaaa taaaacaggt gaagaatatg atatttttgc tcttttttat ttttttagcg
    60181 gttcaatctg ctgcaacaca agatacattg ccggagtttc ccgttcattt tgtggtgatt
    60241 gacggatcgg aagaagtggc cgctgttgcg acattggaac aaatgcatcg cgagattgag
    60301 atactgaata cttatttcgt taccgaagat aaacggacgt tagtgcgttt tcgatttaaa
    60361 tcagcgactt tttttgatga gatcgaaaac acggcgtgtt cgtttgttga ttccgctaat
    60421 gagggcatgt atatcgggcg ctgggccaat ttatataaag aatgtgccga tacgaaactg
    60481 cgcgatccgc gagcgattaa ctttgttatt tttgattctt acacggatgc ccaaggttgg
    60541 cggtcgcgac gaagccgcgg tgcaacctat gccggcgttc cggtggtgtt tattgattat
    60601 caggcgctcg gccacaaagg ttcgctggaa gagcatgaga tgggacacgc gtttggttta
    60661 ggtcatattt gcgattcgac tttgacggaa gacggcggtc aaaatatcat gcatcataac
    60721 ggcgaatgcc ccgatcaaaa aaaatatcgt gcgaaatatt acaccggttt taacgccgag
    60781 caagaagcga ttatccgacg agctatcctt cgtcatcgca aaaaattagg attagataaa
    60841 taatgcctct ctcgccgaag atcgatcgta ttctttcaac agttctgttt ttgggattgt
    60901 gtgtcttatc gttttggatg atcggttggt ttcagcccaa ccatgactac aaagcaaaag
    60961 ccgtgattta caaaccttct atatttgtat atgaagcgct gcgagattcg tctcgtttga
    61021 aaagctggtg gacagattgg gcgggaagcg agccgatcaa tgattctgcg catgtgatac
    61081 atgaaatcat tgacagcgaa aaaagcgaat ttgttcttac ggacaggata gcgtggcccg
    61141 gcattttgct tacgtatggt tacgaagatg ccgagaaaaa ggggttttta gaatacaggc
    61201 tggaatatat gggtgattcg acgcgtgtta cgttgcgtca tcgcacgcgc ggctactcat
    61261 ggatacagcg ctcactattg gccttgcgtt ataccgatga taaactgcga tggcgacgat
    61321 acctttataa tttcaaacgc atgatggaaa acgattcgat ctgatgcggt caattgtgat
    61381 acaaaagatc gttgggcaga ttatagccgc ttaatgcccg catgtagtta gcccgttcaa
    61441 aggctgtggg atcggaaacg gatttttgac tcatgctgcc ttgcagttgt tttaccgatt
    61501 cgtattctct ttcgacgagc catgtttcca tgccttttac aatatccgtg atacgtgcga
    61561 ttccgttttt gagcaacgtg gaacaaacca tggtgaccga tgcaccggcg agtaatgctt
    61621 tgatgacgtc ttcatgcgta tgaatgccgc ctgtcgccgc caggtcggcg cgaatgcgcc
    61681 catggagaat ggcaatccat cgcagagcta agcgtaattc cgttgattga ctcagaataa
    61741 cattcggcat tacatcaagc gtttccagat tgatatccgg ttgataaaat cgattaaaaa
    61801 gtacaaggcc gttgatgccg cagtcatcca tgtgtttggc aaaatgtgca aaagaagtga
    61861 agaacggact gagtttgatc gcgatgggta tgcgaagact ttggcgtaaa gtgcgtgcga
    61921 tttccagata acgatgttcg atagaggtcg cggattcagc gatatcggcc gcaatgtagt
    61981 acatattgag ctccagcgca tcggctccgg cttgttcgat ttttttggca taatccagcc
    62041 acccggaatg cgatgatccg ttgaggctgg cgatgatggg aatgcccacg gc
//
LOCUS       DAOQGF010000006        52409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_236_length_52409_cov_6.322783, whole genome shotgun sequence.
ACCESSION   DAOQGF010000006 DAOQGF010000000
VERSION     DAOQGF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 52409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_236_length_52409_cov_6.322783"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK937_02130"
     CDS             <1..222
                     /locus_tag="PK937_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNH31401.1"
                     /translation="PPFEIDATTVQTNIVIFDVVDRALDAQRVVDHLGSQGILAIPFG
                     ERKVRFVTHRDLTFEQIDEAVSILRKQYQ"
     gene            467..1120
                     /gene="deoC"
                     /locus_tag="PK937_02135"
     CDS             467..1120
                     /gene="deoC"
                     /locus_tag="PK937_02135"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNH31402.1"
                     /translation="MIDHTLLKPEATAKEIDQLCEEAAKYCFASVCINPSWVKRAAQK
                     LARSGVKVCTVVGFPLGATSTQAKALETEIAVSHGAQEVDMVINVGWLKSGEYELVKK
                     DIAAVVRHAGRQVPVKVILETCLLTNEEKIKACVLSKEAGAAYVKTSTGFNKGGATAA
                     DIALMRHVVGQDMGVKASGGVRTYEDALQMVQSGASRIGASASVKIVTGKSGEAAGY"
     gene            1166..2167
                     /gene="fbp"
                     /locus_tag="PK937_02140"
     CDS             1166..2167
                     /gene="fbp"
                     /locus_tag="PK937_02140"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNH31403.1"
                     /translation="MPNRFKTIERHIIDMERLHPEASGELTGLLSDIALAGKIISSEV
                     NRAGLVNLLGLAGGENVHGEAVKKLDMFSHDMLVKIISGGRGEIFMLGSEEMEHPLSL
                     DDNYPDGKYIVNFDPLDGSSNIDANVSVGTIFSILKKDSSTNPKEACRQSGVQQVAAG
                     YIIYGSSTMFVYTAGHGVHGFTLDPGLGEFILSHENIKTPERGKIYSINEGNSNKWHD
                     GIRNYIAYLKEENKSDKRPYSSRYIGSLVADFHRNLLYGGIFLYPADRSNPEGKLRLL
                     YEANPLAMIVEAAGGAASDGHRRIMEIEPTALHQRVPLIIGSKADVETACAFIQGQR"
     gene            complement(2171..2977)
                     /locus_tag="PK937_02145"
     CDS             complement(2171..2977)
                     /locus_tag="PK937_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31404.1"
                     /translation="MEIPAHERLALTGVSGSGKTTLLRMLAGLTEPDSGRIYWQDQPW
                     FDSEKKINRPPERRAVGFVFQDYALFPNMTVYENLAFALSEPDDEAVRRMLQLTELEG
                     LASRRPDSLSGGQKQRVALARALIRKPVLLLMDEPLAAMDVALRKKMQQEILTLWNEA
                     PSMMVWVSHDMAEICRLSNRVCVLDNGRMVKLGNPMDVYHDKHLSGKFRMDGEVVGME
                     PNDTIFILTIASGERLIRVVATASDIESLQIGDRVTVVSKAFNPIIIKQN"
     gene            complement(3043..3711)
                     /gene="modB"
                     /locus_tag="PK937_02150"
     CDS             complement(3043..3711)
                     /gene="modB"
                     /locus_tag="PK937_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNH31405.1"
                     /translation="MDWAPFWLTFKLAGCTTVLLLIAGIPLAYYLAFTKSKWRIPLQT
                     LVSMPLVLPPSVLGFYLLIAFSPKFWFGAAIESWFDVRLAFSFTGLLIGSFIFSLPFM
                     VNPLRTGFGSVHRSLIEAAQTLGKSRGEILYRVLLPNMRPAVVSASIMTFAHTVGEFG
                     VVLMIGGSIPEKTKVASIALFEAVEQMNYSMAHTYALVLVIFSWCVLFGIHVIESKNN
                     ARGR"
     gene            complement(3716..4120)
                     /locus_tag="PK937_02155"
     CDS             complement(3716..4120)
                     /locus_tag="PK937_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNH31406.1"
                     /translation="MNKIKCHIVAIETEASLSLVTMQSANAPLTAVIIETPETASYLK
                     PGVQVYAAFKETEVNLLPQDCRHGSMSNQWTGTIVQIRWGKLLTEVGVQTAIGTIRSV
                     LTTENAKRMSLEENHNLEVYVNAQAVAVWEDA"
     gene            complement(4113..4886)
                     /gene="modA"
                     /locus_tag="PK937_02160"
     CDS             complement(4113..4886)
                     /gene="modA"
                     /locus_tag="PK937_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH31407.1"
                     /translation="MPTSLQHLIKNLFANLCMLMVLNVFELHSQSSVRIAVASNVSPA
                     TKTLMAIFTQQTGIRVEIISGASGKLATQIEHGAPYDIFLSADTIFPNILYEKKLTRM
                     RPRLYAQGQLVAWSARPLYSDDAMGSIDQAEIRHIAIADPALAPYGKAAMDAMKKSGQ
                     YERVKDKLIFGENISQVNQYITTGTADIGLTALSSVLSAPTSLKGYYRVVDSKLYNPI
                     IQSAALLNDKSEARQCFEFIYSDTAKEVFEKFGYTRIHE"
     gene            5142..6149
                     /locus_tag="PK937_02165"
     CDS             5142..6149
                     /locus_tag="PK937_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNH31408.1"
                     /translation="MTTSPILIQKRFQFPPGQNPFITEEQRFFSYNHYIRSRFGQRIH
                     RVTVDAGFTCPNRDGTVASGGCTYCNNDGFSPASQIYRVKKYLKPSTPVHDQITNHID
                     AMKKRFKTDKFFAYFQAYSNTYAPVDELETLYREALHHPDIIGLVVGTRPDCVDEEKL
                     QLLQSIAETHYVSIEYGCESIYDKTLQWVNRGHDFACFIDAVEMSAGRNLDVAAHIIL
                     GFPTETRDEMLAMAGAINRLPVDFIKIHNLHIVAQTPLAFQYKKSPFPVFEQHEYSML
                     VCDFLERLSPNICIQRLYGDAPRAMQIAPRWSTTGQDMNLWVQAELTRRQSFQGIRAT
                     E"
     gene            6234..7394
                     /locus_tag="PK937_02170"
     CDS             6234..7394
                     /locus_tag="PK937_02170"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31409.1"
                     /translation="MISQKTIERLSKIGSRDFFITSLYLNLDHTQKHGQYKIALKDLL
                     KDRRHRLDEFRKHNKITKEQIKSLEDDFVRIENYIQHEFVSKDSGKGLAIFSCSATHF
                     WEILELPQPVPNHLNADFDPNIRILSELLCDYRHYAIALVDSSKAKIVDVNLGFVREV
                     WSVEHAITGKTKFGGVDGTQERNAERAHDESVHKHFKVVATEVEKVFDHSDHMWLILG
                     GRQNFLPQFESLLPAPVRKKIVGHIVIDPEASLNEVLGKAYTIAEEAEAAHEKEIIEQ
                     LRGEASNGGKGIFGLQPTLQSLRKGGVSTLVVTKGYQAPGFVCHKCFFIGTPEEKGKH
                     DTCPICSGPAHNVEDVVEEAITFAYMQGCKVETTNENSRMRIMGDFGALLRY"
     gene            7471..8751
                     /locus_tag="PK937_02175"
     CDS             7471..8751
                     /locus_tag="PK937_02175"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNH31410.1"
                     /translation="MTTTCSVFLTIFVLCFAQAQSSNGQNLDHIEKFNSVKSLFTSRC
                     ISCHGGDEPAAGLRLDRNLYAQIVNVPSNGNPDLHLIQPNSPESSYLYRKVARQPNEL
                     PFADDPMPLDDIPLSSSELQIIREWILSFDPALWPAENQNNTIIQNPATEIVPKFLAT
                     QLINMPSTQTLGNRSAEFRIMHRFGLINGGPDGGNTYGGFFGMDAGAITSINFSIALT
                     NNADILLRRTGTNKDFELGVKYVPFHFNERFPTDVGFFGSIDWISRDDVPARHKISPN
                     LQMMIGSRPFENLSILIAPGIAFKSNHNATITKTVNDTTTQAYRDTRATTAIGLGAEY
                     LIGRGYAISGEYIPRLGGYKGNSFSNDRRYDNWSLAFSYKVRLHVFQLLVSNTQTMHT
                     TQYLPGSSTITKNALYNKGWNFHFGFNIYRQFKW"
     gene            8745..9152
                     /locus_tag="PK937_02180"
     CDS             8745..9152
                     /locus_tag="PK937_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31411.1"
                     /translation="MVKEIKLFSLLVFVMILLSACGELKEESQPTLLVPADVQAIFND
                     QCTGCHSGSSPAGSLNLSVGNAYGQLVNVTSTIEPALKRVVPSDTANSYLVDKIMGTQ
                     TVGERMPDGGPYLSAAQMNIIKLWIQAGAEAKP"
     gene            complement(9197..10798)
                     /locus_tag="PK937_02185"
     CDS             complement(9197..10798)
                     /locus_tag="PK937_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH31412.1"
                     /translation="MNGFSVWAADKSGVIRKDETWEGVITVTGDVTIPEGITVRIRPN
                     TLIRFMANQDDEAGGIDVAKSELIVEGILRAEGQEKFQIRFTSSNFTDPGEKGSTLQP
                     QAGDWYGLIFRRGEDRSILSNCVIEFAYDGITCINSSPRIFRNRIEGNYWNGILCDIM
                     STPKINNNQIQNNGYAGINAKINSAPSINANEITGNRYGVLVQDVSKPLIGDTRLGEN
                     SGKNAIYNNLEFNLYNHTKNVVYAQRNDWGDNFNADRFIHDDDENNKFGLVVFTPVYS
                     TGNISYLEFKNIAADAPTDTKAEQEKRRKEIEDLKRQLAQAKPTDKAGGAGLTESERQ
                     KRAEDARKEKERLALLEEQLKQQEQIRIEQERRLALQKQQEEDQKKTAAAATVKQPVV
                     TVNKETAAATTTPTPEASTTNAASAGTTPKPVESKPAAPVFVPTKMANELDNNPKALQ
                     KVSPVMPDLAKKAKLNGTVSMRVLVGTDGKPEEIYVSKRIGNKDWDQMINDAAIAAVK
                     QWLFEQGLSGGVPVKYWTVVTVLMK"
     gene            complement(10851..14396)
                     /locus_tag="PK937_02190"
     CDS             complement(10851..14396)
                     /locus_tag="PK937_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH31413.1"
                     /translation="MDNKSRKIVEFYLSELLNHVKSVSEHRFAPLEFSITKKIIVSIG
                     QAQEPVRQVESFLNVASLHPVATFLRSNLEKLEKSGYKMSQMITTLENDSERIIQMFQ
                     KILETPGSQDQLNNDLQTIGFSLDLPALRKTYIKQEDTPSPSEGTPKDEVKPISVGVK
                     TEPPASPAIKNDSIFSHNTSRAESTHEKTSVTSASPQSSLLEVAPITQSEAFDHVGDM
                     IGFLSRRTKKQPEKAVAQIVQPTEKVDPKENINAVRSVFSTDRLNSAWETLRDNPTRP
                     RFLISMADGLSQLRAEAVTQNAMPLSEATQRIEETLNAIADNNHKPLNPEALAVLHDL
                     IQQVENNNSDLKSLAPAEILRLQQRFTGSFNMEKPEESPVQSSSSNAQLVAENEIAID
                     RVAIQDDDWNVFAEDVRFNLELAQSALVRLKKATHDTEAIKDLLQVYRALSTNTRLLK
                     INLLHGHCDAVFQIFKQVVYSQSPVVAELITLVTRSTRSIEILVNGQTIQSDEWRWLN
                     DQSVRFSDYKQATTLVTPAKTTEISAVPSEQTKVVAPEVVKTEIKTAKESSKPISDPK
                     SVVAQNTDWLEGFQSVVAKFNDSPIAQHIKAEKVEPNEKDTKDSTLQIKEETRTQSVT
                     PTKTILPGVEEKPKFVAEAVVQNKIEVKPVEEIKKATPDKKTDDRQSTLASPVTDDTK
                     TIEIEVPKALLAMMAAGDADDELNGLRIPSFAERLNTEPKDIITGGRRTVKKKRSELP
                     KEQVKPIPEKASVKEALKTMTVAPPLKTEKVESAVVEGKLMLEESAFDAVDPEILDIF
                     NQEAAEYFKKFDAAFARLKQSLVDDVAIRDLERTSHSLKSSARMLGFERISGLSAAVE
                     LITERCVEKEISVDATIVTLLESITTALRLIAARKAADVTQVVQQLFELENSLNASGT
                     FTGAIPGSASLAKHKGIETKAPEKKPEPVIEAVKPAEVSQPAPKENYFSKLGIDDEIV
                     DIFKEEVSTYFKLIGAALSTLRNNMTHRQSMRDIEKAAHSLRSSAKMLGFQKISNLVK
                     PMETVAEKMNEGELRVTSELIDLYETSIERLKQLTAGTDVDVDDVVGKLQSYEQKAPE
                     ATDIKPVNDEDVRARIKAELTQEIKLDKVPEKAVTPNEKVISEKKTETKVKAVKKPAK
                     AFDDVPIEKDAILKNLLKSGPQLLDEMFEATQHGV"
     gene            complement(14468..15265)
                     /locus_tag="PK937_02195"
     CDS             complement(14468..15265)
                     /locus_tag="PK937_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH31414.1"
                     /translation="MKKIEWRVLFSVDGKRNVADIAEKLDRDATLVEEILQKLSAEKL
                     ISGGAPAPAAKPAKEKAPSKKAVKEDAPKAKAEPKPEPKAEPKPEPKIEKAPEPKPAP
                     KKEETDILGSMDTPKVEPKSEPKVEKAAPKPVAPAASASAGGRKILVVDDSIVIQKMV
                     EIALENDNYNLTSAMKGEDAINKAKEMQPSLILLDMMLPDMTGVEVMKALRETGGYFA
                     TVPIVVLSGKDSPQDKDAAISNGATDFLNKPFHDEDLLAKVHEYLGK"
     gene            complement(15422..16297)
                     /locus_tag="PK937_02200"
     CDS             complement(15422..16297)
                     /locus_tag="PK937_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNH31415.1"
                     /translation="METKELLRNVRRIEIKTRGLVNNVFSGEYHSVFKGRGMSFSEVR
                     EYQFGDEIRFIDWNVSARLDKPYLKIFEEEREQTLMIIFDASASGDFGTNKQTKRELM
                     IEMAAVVAFSAIKNNDKVGLMIYTEEVEKFIPPKKGRSHVLRLIRELIAFVPKARGTR
                     IAAPLELTSHILKRRSIVFVISDFLDQGFDTSLRTLAKKHDVIGLRVADQREQNWPLV
                     GLVTLEDEETGALIDIDTADQQFHAILRQRVDAEENRLKEIFKSSRVDLINIFTGSDY
                     AEALISFFRKREKRH"
     gene            complement(16339..16986)
                     /locus_tag="PK937_02205"
     CDS             complement(16339..16986)
                     /locus_tag="PK937_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNH31416.1"
                     /translation="MIKIEVMFFDLDGTLVDSLDDIGASVNHTLTTLGYSALPKEKLR
                     RFVGDGMQTLLARALSEFEKPDNDQLMTAVSIYRTHHDEHCLDFVRTYDGVLDVLTHF
                     NHCRKFVISNKAEVFTRKIIHGLGLSRYFEGVYGGGDSFANKKPHPQAIQAMLEKTGV
                     HPDRAMIIGDGPQDIEAGRRAGVYTCAVRYGFHAVAPEGANFYIDRMIQLKEFVA"
     gene            complement(16983..17663)
                     /locus_tag="PK937_02210"
     CDS             complement(16983..17663)
                     /locus_tag="PK937_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNH31417.1"
                     /translation="MDQRTVAAMMTMGNIITASLWGLGIGFINSIPFGPINMTIVDQS
                     FRRGFQPAMMIGLGALVVDIGYCIIGVFGMSALQQSLIDMLQPAGMPVLVFLGYRLII
                     TRHNGNVYHERTEFSAGLLMKNFINGCIMYLCNPLAIGFWIAAAGFMYAYQFLQSSLQ
                     SQLAFIAGMAIGTAAWFFLLAEWVAVRRQTMKETTIRRIAVITGWVLIVSGFILGWRY
                     VSYKGWIA"
     gene            complement(17636..18760)
                     /gene="ribD"
                     /locus_tag="PK937_02215"
     CDS             complement(17636..18760)
                     /gene="ribD"
                     /locus_tag="PK937_02215"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNH31418.1"
                     /translation="MPSPKTHEYYIRQTLKLARKGVGKTKTNPLVGAILVRHTPNGDQ
                     IIGRGYHTGYGKPHAEVEAVRDAQRRGVTDFKNTILYVNLEPCCHTGKTPPCTSLILQ
                     EKIPRVVFGIKDPFPAVAGKGAALLKKNGVRVEWGIAESECRIENRFFLKHVETGLPW
                     ITIKTAQTLDGKTADGFGASKWITNEKSRREVHRLRAGHDAILVGAQTVMADDPQLTV
                     RGVHGSSPRRVIVDGRLRSPLTARVFTDAGASETIVITTLGANKHKKRQLASLGVTVV
                     EMSSVRNRIAMRSIFRTLLRKFQVASVFVEGGADIHGQILNAGLCDEIHVFTAAKILG
                     HGKSSFDGLRVKRINNAVVLAEPRVRILDSDIWISARWQR"
     gene            18928..19476
                     /locus_tag="PK937_02220"
     CDS             18928..19476
                     /locus_tag="PK937_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31419.1"
                     /translation="MHKALHKSQELFTATWGEISTFWGINKVMGQLYALLFASAKPLT
                     LDDMCERLQISRGNASMNIRALKTWGVIRKVRVQGDRKDYYELDEDTGKIMTRFVRER
                     KRRELEPALETMRRSAILLRTAIPAMKNGERKQAQIFLKRIENMLMVSESINTLLERF
                     IHGESISINQLKTIPIAWKKGA"
     gene            19493..20545
                     /locus_tag="PK937_02225"
     CDS             19493..20545
                     /locus_tag="PK937_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH31420.1"
                     /translation="MNIIEREPWIQSFLQETDAVIHEVFENTGSAEFKDILKTHHQGG
                     KRLRPMVIAASAQLGDRPAHHLAYGAAALELLHLASLFHDDVLDDTTMRRDKLTARKH
                     IGNLNSILTGDYLLAEVLHTIVEKLPTPLVSAFLDTIKRMTRSEILSKKLLNNLQIST
                     SEYVQIIENKTATVFALATSLGIRLSTDDPVRIRQLSIYGMNLGMAYQIIDDMEDMTG
                     ASELTDDDLSHGYIGMPVIELLRAVPLNQKQEVLQMVRATQPEQRAALIRMMKSFSIF
                     KVVHARVQHYLNISANALLDIKANTPEQRISLEFLKGLCGYLADKSLKAVDTYESADH
                     TGTQTVSASTTVAATA"
     gene            complement(20535..20888)
                     /gene="cutA"
                     /locus_tag="PK937_02230"
     CDS             complement(20535..20888)
                     /gene="cutA"
                     /locus_tag="PK937_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNH31421.1"
                     /translation="MSNVTSNVTARTAIIVYMTASSDAEAKHIADALITEQLAACCNI
                     CPGIRSIYMWEGKLCDEKEVLLLIKSFEDCFEALAKKIKSLHSYKVPEIIAVPLTKGD
                     EAYLRWMEEVVPSKP"
     gene            complement(20925..22727)
                     /gene="lepA"
                     /locus_tag="PK937_02235"
     CDS             complement(20925..22727)
                     /gene="lepA"
                     /locus_tag="PK937_02235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNH31422.1"
                     /translation="MSTEKIRNFCIVAHIDHGKSTLADRLLEYTKTISKRDMEAQVLD
                     NMDLEKERGITIKAHAIRMEYKARNGQTYVLNLIDTPGHVDFTYEVSRSLAACEGAIL
                     VVDASQGIEAQTISNLYLAIDAGLQIVPVVNKIDLPSAQPEETCKEIIDLIGCKREDI
                     VLASAKAGIGIEDILEKVVESIPAPVGDPTKPLQALIFDSVFDPFRGAIAYVRVFQGE
                     LVTGTHMKFFSNDCRYDADEIGYLRMDKIKADKISVGEVGYVVGNVREITHIRVGDTV
                     TDAGNPAPAALEGYKDIKPMVFSGIYPTVSEDFEELRTSLEKLKLNDSSLIYEPESST
                     ALGFGFRCGFLGLLHMEIVQERLQREFNISMITTVPSVRYQVYKTNGEVIEVSNPSMM
                     PSPGIIDHIEEPYIKAQIITPTEYIGNIMSVAMERRGVYRNTNYISPTRVDLTFEFPL
                     AEIVFDFYDKLKSCTKGYASFDYEYIGYKEGELVKLDILINSDPVDAFSGIIHRDKAY
                     EFGRKMCEKLKELIPRQMFEVVIQAAIGSKIIARDQIRAMRKDVTAKCYGGDISRKRK
                     LLERQKEGKKRMKQVGKVEIPQEAFLAVLKIGDE"
     gene            22880..24751
                     /locus_tag="PK937_02240"
     CDS             22880..24751
                     /locus_tag="PK937_02240"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNH31423.1"
                     /translation="MTVLPILSHHTRKSFLCLSLSLGLAYGSVAGYAQESSETAFMHA
                     VRSYQNKNWAESRDKLSSYLAQYPDKPATPAAQILLARTYLRLDNPRKALQFINQYSA
                     GFSSQSPYATEFVFTKAEALDVMHRSLEAIQTLLPLASGKTDAGLRADSLIARLAEVR
                     LSTVQLKELAGSLPSSPVKEKLQILYATKLAAQQRYTRASRGMDVFLRENPRSTYYKN
                     AQQVFRRNQNKEDGPVRIGLITSLSGTNAEAGLALKSGIELAVREYNTTHSTPLEIVT
                     YDDRSDVLQSVLAAQALCREGSISVIIGPLESDAMSAASVVCEAAQIPIISPTASQTG
                     ITGIGRFVYQANIDIESRAAALAEYAIKKAGHRTFAILSPNDVYGATSTKAFTDRITQ
                     LGGRIIASESYFDNTQDFKSQLLRLRKLGQIEEARPGLDYRITANLNFRQIDSLYKMI
                     FPEDTTTTESSSDYNMPSRGIDALFMPIYDDEIKYIAPQIAYYNIQTKWLGGDHWNAL
                     DELRRQNKYVNGCIFVGEFFSNPDAGPTKQFIKNYTAAAKKRPTPEAFYGYDIVGVLG
                     VIIERGAQLPDEVQNELQNGITYEGVHNQIRFDKGRRVNASVHLLQFKDNTIHKIEP"
     gene            24772..31476
                     /locus_tag="PK937_02245"
     CDS             24772..31476
                     /locus_tag="PK937_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNH31424.1"
                     /translation="MRQIRFFILLIFTSIPLAFAQSDSITISEVMFSTSNTLDFIELY
                     NYSKTQTIDLAKYIITDGAGNNDSDRISSAGEGLLLYPGQFAILLDPDYFLPPNASGR
                     VYDISIPDTARIVTIDTNQTGGFVLGNGSFSNTTAETIHLIKVQSGIPRDTISRYTYS
                     LGNNTSYSDEKITLNNDNNSSNWTNSIYLNGTPGAPPETNLSINDHRMIFTPPDPLTG
                     ASVTIGAIVTNKAIATSDSFRVLFYHDADKNGTADVSELLDSVSYNVSLPRNTSDTIF
                     FTAPSIASGINYYIARIGSIYPYADTVLSDQSLRDSVQTTPAYNVSWSASSVFSPSSP
                     ATGDTVTYARAIYNAGIADITHFAAYLYIDQNHNGMIETDEKVDSANYNDSLPSGDSV
                     VIYFTIPDVQRGKLHLLLDLPSDSLGPLPDEQPADNRTSDSANIRAAMSLTLTSASAL
                     KPSYNYIADTMAIKAVVHNNGSTSASGFSLILYHDANNNGLPDTNERVDSVRYTSSLD
                     AAASDSITLHWLPTSEGNISLIVKLDSTTVQPLTDEDGSDNTHTLNALIYPRPDSITF
                     SEIMFDPLGSEDSNEFIELYNFGTHAVDLLNWKIADSLGYDAIQDAGNGTVLQAGQYA
                     IVFENEYTLNTGRYTSRIPSSALVLTINGSTFGSSSSGLVGTYPKQLFLVRSLGDTIS
                     TYRYSLDNTEGHSDTKINPSAANASVNWMNSLITDGTPGAHAEVNLSVLGQRLRFAPS
                     EIPSGTQTVISVTVKDSGIAPVNIFKIAMYHDTNRNATPEPEEIIDSLVFSGQLYYGD
                     SVIFTANTPTLSYGVHYFIARISQGEVSPYGDTLYYNDMLTDSIRTQPSGDIALNRGR
                     LILGEIATRGNSFPIGAYAYNMGTQPVQSFTVRWYEDRDGNYTTAPEERIDSSTYAGN
                     LLPGDSTFLQINYPNATYGKHRIMVEVISTSVQPIVDEEPSNNLLVDSLEAGARARDI
                     LISEFMYLAVTDSIPEWIEIYNPSALSISLMNWQLYDKTSHVVLTERSDTLHTGQFGI
                     IVKDSTLFHKKYGEALSDVLYIYPTSMPSLNNTGGDIIKLSTPAGLTVDSLNYLTSWG
                     DDEGISLERKLNDSASYSMSNWALSSDPSGGTPGTKNTATPQLHDLSITSRDILWTPA
                     YPTQGEVFQIKATVRNLGVTSSGASAILLYAYSGADSTLLQTTNSPAILPGDSLVIVS
                     SPITNGPQTYVGIQIDYAEDERLSNNFAKRFVPAATPRQTVIINEIMYKPGTGHTEWI
                     ELYNRSQNAVDLRSWRISDGNADLTAPATAKTITVQSVILQPDSFIIIAKDSVQFSSQ
                     YGTPLHAVYLSSMPALNDPGDMITIQDSLGSIVDSLVYLSGWGGSSDRTLERRFYDSV
                     SYLTSNWLSCESPIGATPGHPNSVKPLHYDISLNASPLHVTPSYPRIGEPFTITAPIY
                     NQGLEPVNDASLRWLRIRPQHDTLILSEQIIGMLNAGDSLKTSYSDTARQIGDSIVAL
                     ILSVSDERTLNNKSAVPLITSARRNALVINEIMYDPGSNRPEWIELYNPGADTVDLKN
                     WRLADESTIANPKTFAGTSLRIPSQHYVIITKDASALALSYPLLSSPVVTMTSFPTLN
                     QTGDLILLTDSLSVTVDSVFYENTWGGANGFSIERISATDDANSPNNWKTSEANAGAT
                     PGGMNSTTPVALDLSIRTNDVWFDPPFPSPGQPVQIHMRIRNKGLQNITNAAVVRVYN
                     DINNDSIPQITEIIDSTTVPVLLAGDSQSVTIQWTIPASASRRLSKAVSMGSRKIIIQ
                     LDFASDERTENNLLFTALPLGTPFQSIVINEFMYETDTSRTEFIELYNPGTTAWDLKD
                     WKIADGSSVVSIADVSVSFLSGAYKVLAPDSSFFRKFSTTPASSVIIVPGLPSLNNTG
                     DAIRLADDLGATVDSLTYSNTWGGSKNISVERIDADVSSDEPNNWTTSLTAQGHTAGS
                     VNSIVSAEPFVKQSLIINEIMFSPLSGEPEYIELYNRSETAISLLNWAITVGDDKTLL
                     AQTDISVPPHAYVVIAGTKTFPDRYDLDKTQIIVPLTNLAALPANGTALVLRDWVGTT
                     IDSLAYTELWGGGDGYALERIQPDAASDLMTSWRTCVFVEGGTPGKINSVYNGDKPKK
                     IRITADPNPFLTDVAENTTITLEMPVTYARVTMKIYDSQGRLMITLLNNQPTGAHREV
                     IWDGRDKNGQRVRMGIYIIFIEAIEESGGFNKTAKQTIVVGKRL"
     gene            complement(31559..32353)
                     /locus_tag="PK937_02250"
     CDS             complement(31559..32353)
                     /locus_tag="PK937_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNH31425.1"
                     /translation="MIKRLLFILVGVQSLWAQTEYPEAISDNSFMLEEAYNQEARVVQ
                     HISGLIYTRPSKDWEYGLTQEWPMFFLPHQEKHQLSYTLPYQWLHSGPSGFGDVAVNY
                     RYQWMADPAGYSVSPRFTVLLPTGDDNKGLGSGKMGYQIGLPVSKRFSAQWAAHVNVG
                     MTMVPGVDVGSKKKIQKAYNIGASAIWLVKSNLNVFVEYVSVFEDEVDGTSLKNKGTH
                     TINPGFCFAKEIGSMQVVPGLSVPLQLNGSSKTKQVFVYLSMEHPF"
     gene            complement(32367..33071)
                     /locus_tag="PK937_02255"
     CDS             complement(32367..33071)
                     /locus_tag="PK937_02255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31426.1"
                     /translation="MKTALITGASSGIGLATAQKLTEMGYRVYGLARNFDKTDWRHAN
                     FVPIVCDVRDIKALEEVEKKWPTEESLDVLIHSAGFGYFAPHEEIPLRTLQDMVTTNL
                     TAPLILTHLFLRRIREQRGFVIHIASVTALEPSRMGAAYAATKAGLRHFSASLFDECR
                     KQGVKVVNINPDITRTAFYDNLNFSPSDETDTYIEPQEVADTVAHVLTQRDGTVISEI
                     TIRPQRLHVIKRKHHH"
     gene            complement(33068..34087)
                     /locus_tag="PK937_02260"
     CDS             complement(33068..34087)
                     /locus_tag="PK937_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31427.1"
                     /translation="MHAWSGFDKSSFSKHFSRIYIEDGAEAYPDAQTILARFPTARRI
                     PIRHYKDVFNRKHQDFQLQKRAMDIILAVKREGYLYAGSPQTPSFGHDRFFYNAMVLN
                     CVYNCDYCYLQGMFPSAHLLFFVNPDAFFDSVVAELAKGPLYLCVSYDTDLLAMESFL
                     PQCHRWIHFAAQRRDLVVEIRTKSGAYDAIRDTPVSDNVILAWTCSPDEIIKVYESGT
                     PPLDTRLSAMAQAVKDGRKVRLCIDPILKIKDWQAIYSRCIEYIFERIPGAALHDVSL
                     GVFRMNKNYLNNIRAMRVDSDILFYPYDEKDQTMSYTDLDRESMLDYIRKTMSAYVQE
                     ERIIL"
     gene            complement(34047..34880)
                     /locus_tag="PK937_02265"
     CDS             complement(34047..34880)
                     /locus_tag="PK937_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31428.1"
                     /translation="MKKNTKHGIVVALRPEAAPLIRAWRLKKNIAHTAFEVFENETIS
                     LIVSGIGKIKSATAATYLYTTGHLHTLWNFGICGASDTTLDIGSFWRIHKITDVVSGR
                     DYYPDGSIRLTMGESVLRTVDRPQENQCVGSADHALYDMEASGFFVASTRFLPLERIH
                     VLKIVSDHLQIKGMTSDFIENLTMQCLPKFSSCLDVLSAMRIDKETLSVNEQETMQHI
                     FEMINPSHAQRIALFDAARSYKIRNGYLPGDLMDMTQWAGENKQERNARVERLRQKLI
                     Q"
     gene            complement(34864..35274)
                     /gene="mce"
                     /locus_tag="PK937_02270"
     CDS             complement(34864..35274)
                     /gene="mce"
                     /locus_tag="PK937_02270"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNH31429.1"
                     /translation="MKLEKIDHLGIAVPDLEKFIELYKAMGFEYHGREIVADQKVETA
                     FFKVGESSIELLAGTDPESPITKFIEKNNGKGGIAHVAIGVTGIEEKLADLKAKGFQL
                     IDEVPKTGAHGARIAFVHPKSTGGVLLELCEKKH"
     gene            complement(35280..36155)
                     /locus_tag="PK937_02275"
     CDS             complement(35280..36155)
                     /locus_tag="PK937_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNH31430.1"
                     /translation="MNAKIIDGKQIAESIKREIAQEVEALKIKNVQPKLVVVLVGEDP
                     ASQVYVRNKGLACEQVGMAHETIKQPATMPEKDLLALIDQLNKDPSVTGILVQLPLPK
                     HINETLIINAINPAKDVDCFHPFNVGRLAIGDAIFLPCTPAGVQELLMRSEADPSGKH
                     TVIVGRSNIVGKPLANMLVQKAKGANSTVTVCHTGTPDMTYFIKQADIVVAAVGRAEM
                     IRGEMLKPGAVVIDVGINRISDPSTKSGSRLVGDVHFDSAKEVASAITPVPGGVGPMT
                     IVMLLKNTLKAAKQG"
     gene            complement(36213..37892)
                     /gene="ggt"
                     /locus_tag="PK937_02280"
     CDS             complement(36213..37892)
                     /gene="ggt"
                     /locus_tag="PK937_02280"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNH31431.1"
                     /translation="MFLRIFFLTALIIFPSCQNSHQTSRISSSVTAQNGVVVCVDQNA
                     ARVGLEILQKGGHAVDAAIATAFALAVTYPQAGNIGGGGFMMVRTSDGNVKAIDYREK
                     APYQAHARMFLNADGSEDTITSNYGYKVAGVPGTVRGLEKAWKLYGKLPWSELINPAI
                     RLAEEGFILEAAEARRLSEFAPALKRFDETAHMFFKPDGSPYAEGELLIQKELAATLR
                     AIADEGADVFYRGKIATQIADDMKAHGGLITHADLAAYEAVVREPIRGNYRGYTIYSM
                     PPPSSGGVTLIEMLNILENFTLHDDTISTRRVIETMRRSFLDRLVYLGDPDFSDIPVS
                     ALTDKALARKLASWIDSAHINAKMPALAMKQEPTETTHFSVIDKNGNCVTNTYTLEDN
                     FGSKAVVTGLGFLLNNEMHDFDVFPDSNRFPGHGQKPNRIEPHKRMLSSMTPVIMLKE
                     DKPFLITGSPGGKTIINTVLQIIIGMIDYGDSLRQAVDRPRLSHTWSPNVLSYEKDKL
                     DETRRAFLLRNGYILSEKPFIGDAHSIWIDPRTGYYHGEADQRIMGYAAGY"
     gene            complement(38031..38507)
                     /locus_tag="PK937_02285"
     CDS             complement(38031..38507)
                     /locus_tag="PK937_02285"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH31432.1"
                     /translation="MRWSLAIFVLAGLMSSGCNKPGSGGAVDFTIKDLDGNTVSLSQY
                     RGKTVLLNIWATWCGPCKKEIPDLIALHNEMKDKDVVVLGVLLESESAEASKPTVKEF
                     NINYPVWYGDDAFAQQFQVQAFPTTVIIDKNGKSVKTMIGLQSKAKFEAAVREALM"
     gene            complement(38525..40516)
                     /locus_tag="PK937_02290"
     CDS             complement(38525..40516)
                     /locus_tag="PK937_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNH31433.1"
                     /translation="MMYKTKRTIVAILTYLIFSVIPARAQTNVVKLSTREAIENGQPV
                     LEIVADIDSKYHVYGMEKLDGPIATEFKFTLPEGTELVGEVMSPEGKRHHDEGFGIDV
                     TWHSGKTVFKQKLKLSATAKTGDIITVAMTYQACTESYCLPPKTIRATHTISQQWIGL
                     SAGTIKPINKINPNQAVGSISLQSHSIAEPENKSSASHDSVAQTVPDKVSTIQERTAS
                     YESLRKESLGSFIGLAFLTGLLALLTPCVFPMIPITVSYFTKHSASSRAGAVRQSVIY
                     VVGIIASFTLFGWLMSLVVGATGAQNFAANAWVNLAIGILFIVFAMSLFGVFEIRLPS
                     FIVNFSQQQANTGQTIGTLMAGVTFTLVSFTCTVQFLGLLMVAAADGEWFLPVIGMLC
                     FATAFSTPFFFLSLFPQYLANLPKSGGWLHATKIVMGLVEIAAAFKFFSNVDLVWDLQ
                     WLTRPAMLSIWITLFGVSGIYLLGKIRWSVEDEVEEIGFGRTMTGLLFISFTVYLLSG
                     LFGNPLQADMDAYLPPSDYGVVRTAGNAKSTESHETWIEDYEAALREARTSGKPLFID
                     FTGKTCTNCRLMEKTMFTRADVQFYFDRMVLTRLWTDFGENMERYQDLQQRLVGSVAL
                     PFYVIITPDEKVLARFGGLERDPEIFKSFLADGLQKSQK"
     gene            complement(40506..41588)
                     /gene="rlmN"
                     /locus_tag="PK937_02295"
     CDS             complement(40506..41588)
                     /gene="rlmN"
                     /locus_tag="PK937_02295"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNH31434.1"
                     /translation="MKNIAKNQNLKPETINIFGKTLSELESFFEALGEKKFRAVQVFQ
                     WMYKRGVTNFDDMSDLSKSLRDKLHASAVIITDTVIKIQTSGEKENDVTRKILFQLAD
                     GEKVEAVYIPDEGRKTVCISSQVGCAVACQFCATGWMGFRRNLSVGEIIGQLMYIRRE
                     IDPEISNIVFMGMGEPFLNYENVMNAAGIIISEHGPGLAPKRITISTSGIIPKIYEFA
                     DADLPYNLAISVNATTDEVRQKVMPITKKYPLKDLLESIQYLNARRKNPATLEYVLLA
                     GVNDSDDDAKRLRTMAKQLGKCKVNVIPYNPIEGPDLKRPSPEHIARFMKLVSGISSP
                     LTLRRSRGRDIAAACGQLAIKEKHDV"
     gene            41677..42312
                     /locus_tag="PK937_02300"
     CDS             41677..42312
                     /locus_tag="PK937_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31435.1"
                     /translation="MNFYARVTVFLLSLVSILLTACSGKSYSTDTPENCHNAVVTAIR
                     THDFGALYTLIDTAIRSDVRTYFHNTRSVLSMIDSSYPASQHIAARGDLTLRFSNDSL
                     TMAHFEKISNDTILFSDVSSRSIGNVSWWENIIQAYRAHPEKIIEEDGFASVITSAGD
                     TVLYQRYNDGKWYCLLPVHTFWKTLATVSRQNLDVTRRNSIVFSSALRDTL"
     gene            42309..42950
                     /locus_tag="PK937_02305"
     CDS             42309..42950
                     /locus_tag="PK937_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31436.1"
                     /translation="MSLKLPWDIESLHLICERDEESWLHGGTHYFHENNSSILAVAHL
                     DCVQAYAGFDFEETDGHSTVHCTTLDDRLGVYIITELLPSIGVTADVLLSTDARHGES
                     TAQMFIPEKKYHWIFSFDRKGSGVVLYQYGDAEILQLMQKYGFEVHEGYRSDIVLMDH
                     LECKAFNFGCGYHEPLTDKSFAVLDESEVNILRFQQFYNDHKNTLMPHVPSYL"
     gene            42990..45716
                     /gene="polA"
                     /locus_tag="PK937_02310"
     CDS             42990..45716
                     /gene="polA"
                     /locus_tag="PK937_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNH31437.1"
                     /translation="MSERLFLLDGMALAYRAYFAFIHNPLKNAKGENTSAVFGFINAL
                     IKIRDEEKPDYIAVAFDLAEPTFRHKQYSEYKATREKMPEDMVPQLHYLKELTEAMGV
                     PILEYPGYEADDVIGTLSVRAAQQGIVAHMVTGDKDFMQLIGPNIVMYNPLKKGGEKE
                     IIGRDGVLEKFGVEPSQVTDILGLMGDAADNIPGVKGIGEKTAAKLINQYGSIEKIYE
                     QIDSIKGKLRENLEQHKEDAFLSKSLATIALDVPLREDIHDLRGRTPDHQKVTRLLTE
                     LEFKSLIKKILKNESDLFVEDTPLQHERTPEPARNYHTLQTEVAIKELVTKLTNSGGF
                     AWDTETTDADPMRAELVGFSFSWTEGEAFYIPCNGDVPVDTICTLLKPVLEDSTIPKW
                     GQNTKYDIIIVSHYGIEVQGTAFDTMIAGHLLHSDKEVNIDALAASYLGIEKIPTTDL
                     IGTGKNQITMRDVPIEKVSAYANEDADCVVRLKNVMSPLLKETHSAKIFNTIEMPLIP
                     VLQRMERHGVWLDLEILSTMSKDFAIQIIQEEQRIFKEAGTDFNMNSPQQLGEILFEK
                     LQIHKLAGIEKPKLTGKTKQYATDVKILEQYRTLPIVEAILNYRQLTKLKSTYIDGLP
                     PLRNPRTGRIHTTFNQTIAATGRLSSTNPNFQNIPIRTDMGREIRRAFTAQEKGWVLL
                     SADYSQIELRVMAHMSGDENLIAAFSEDADIHTSTAARVFGVEANEVTKDLRRKAKDI
                     NFGVIYGISPFGLAQRIGMSQAEAREFIHSYFATYPKVKSYIESVIAKGKEDAFVTTL
                     FGRRRYLPDLQSKNFTVRQFAERAATNTPIQGTAAEIIKMAMLKTHAALEKSSLSAKM
                     VLQVHDELVFETPQAELQQLEALVRSSMESAVQMKVALKVDMGSGDNWLETK"
     gene            45959..46792
                     /locus_tag="PK937_02315"
     CDS             45959..46792
                     /locus_tag="PK937_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31438.1"
                     /translation="MDHTFADACAKLAETYYRASLQHKTNKNTKMHTAMLDQSKRFML
                     MAFDAQASNGNAHFVRGLLAFDNKAYDEALRDFEHAEMYGYKSFELHSMMAYAYNEKE
                     KTALCVEQYQKAIAIKPEDMNTLYNLGELYFQLGNYNMASDYFSKMVKIDPTHSQARI
                     NYALSVWKNGNPNLGKSMINQLLDTDGKTPVNLYNAIAWQLIDKDIDIAMGLKLAQAA
                     NGMKPNNVESIDILGWGYYKNGEYTKAVEYLSQSMKKMPSDEVKNRLKMAKDKLEESK
                     K"
     gene            complement(46829..48685)
                     /locus_tag="PK937_02320"
     CDS             complement(46829..48685)
                     /locus_tag="PK937_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31439.1"
                     /translation="MFSRVLLTATFYVAVFLFKTVPISAQSGDPLNLAQSLEADGQFE
                     KAVTIYEKLYQKDPSNIVVADRLKRAYKTLGKYELRERVLLAQIQNDSSQIAFWAELA
                     DNYYKLKRPERAATIIQKYISTEQSQIGNYRVFASILMENRRWDELERVYKSARKTLR
                     NEKLFIAEMANLQQYRGDYHAATLEFLSFWSYGMGTFDYVRQQILQFSDVSADLDTIV
                     HAIKIYIQQLPKEPKLLKLIGELQFKSGRMDAAFATTIQLDEQFQKQGTEILAFADMA
                     FGAQRYTVAEEAYKSFLYQYPNAPQAEMGLARCWENQDARASVVKAFQDTSLGQAMVA
                     ELRYTNQAIEVYKNIAEKYKNLEWAAEAHYRLGEIYLNRMFDVDEAIRHYEAVYRNFA
                     KNNFAIESLFRLGECHLIRGEIENSAQRYKEAMRINRTAIYLERAEFALAKIDFYNGH
                     IDTCYKRMQSLAKVPNGLYVNDALSFILLLQESKKNLSFQKALAKSDLLTEQRKYTEA
                     LRELNHISETLEESRLKEWVLMRIGITHERLGQYNEAVSTFYQVLTAEPQSIQADLAL
                     KKAAEIYDYKLNRPDKAIQLYRQLLVKFPKSIFQNEIRKRLRLVEQALGGQS"
     gene            complement(48697..49554)
                     /locus_tag="PK937_02325"
     CDS             complement(48697..49554)
                     /locus_tag="PK937_02325"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNH31440.1"
                     /translation="MTFNPENKRLYFLWLFILHVLPAVGQYDVQEIERTDEKELKVRL
                     EYAAGKLSLKKISTDKLARITIQSEDREVKPTVDYEKNGTTGMISMRMDEENNVGFFD
                     VGNQDIQMYLTDKIPVSFKIDLGACESEMDFTQLRVRDLTLNMGASSSNIRFSSLNRD
                     RINKFRIEAGISKLNMYGLGNANFEKLEFEGGVGNYMLDFSGTMTQSASAHITVGIGS
                     LIIRLPQNLAVKIHTDESYLSSIRIDQNDFVKKRDGLYISKGFDAADAVLELHLETGL
                     GKLRVESIK"
     gene            complement(49554..50333)
                     /locus_tag="PK937_02330"
     CDS             complement(49554..50333)
                     /locus_tag="PK937_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH31441.1"
                     /translation="MAKRAIKKNKPTAKAKRKPVQRSLKTPLQSVVSEQAPSKPKLDD
                     DLDIDVPITPEDPNRLLLEKREKQKKEDFEFIARAVGGDQKAFELLMNRYRPQIFGLM
                     YRMVHKSEEAEDLVQEAFMKAFTSLSNFNYEYAFSTWLYKIATNNCIDHLRKKKLQTY
                     SIDAPVQYKDSEYQIEIPDLTYHPEKEMMRNERTQMIKGAIEELPEKYKRVIFLRHSE
                     ELSYEEISEMLNIPIGTVKARIFRAREMLNKFLKGKIDKVL"
     gene            complement(50363..51688)
                     /locus_tag="PK937_02335"
     CDS             complement(50363..51688)
                     /locus_tag="PK937_02335"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNH31442.1"
                     /translation="MKKRNRQELTGDEIRWLIRSYTDGEIPDYQMSAWLMSVFFNGMT
                     FRETTDLTLAMRDSGVVVDLSSIPGIKVDKHSTGGVGDKISLILAPLAASLGVVVPMI
                     SGRGLGHTGGTLDKLESITGFDIFFPMEKFIDVLRSIGVCMIGQTAEIAPADKKLYAL
                     RDVTATVESIPLISASIMSKKLAEGIDALVLDVKVGNGAFMRHFDDAVNLAQHLVSIG
                     ESAGKSVVALLTNMDEPLGEGIGNWLEVRESLDVLRGGGPQDIIQLTLAQTAYMLMFA
                     NPGMTYDEAYARCKDNLNNGKAFDKFREIVRVHGGNLSYIDRPETYPEAECKTSVYAQ
                     TDGFVTTTDTLQIGLASVAIGAGRSKKEDTIDPTAGIVIKKKVGDAVQANDLIAEIYH
                     SPRVDGKAVAHRIQSAYRIEYNAPDKKPLIYGILDKNGLKEIENNRNLE"
     gene            complement(51696..>52409)
                     /locus_tag="PK937_02340"
     CDS             complement(51696..>52409)
                     /locus_tag="PK937_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH31443.1"
                     /translation="FYDGMMEHVDYRTWSSFIHQIFERYGSGISTVCEFACGTGNFAA
                     AMAAFPYTMICSDRSETMIMTARKKSVAQSGKIHFFTGDMLSFKPTVECDAALCLYDS
                     VNYLMKAEDILNFFNHVSECITNRGVFVFDVCTEKNSLDHFDQRYELDRTHHYKRRSY
                     YDRVSRTQFNEIEAEIDGTLFHETHQQRIYSLDEIHAIIRASPFTCVAQLANLGFSEG
                     SEASERVHFVLRKKNYVAI"
BASE COUNT        14212 a        12704 c        11156 g        14337 t
ORIGIN      
        1 ccgccttttg aaatagacgc cacaacggta caaaccaata tcgttatttt tgatgttgtg
       61 gatcgggctt tagatgcaca acgtgtagta gatcatctcg gttcgcaagg tattttggcg
      121 attccattcg gggaacgcaa agtacgtttt gttacccatc gcgatcttac gtttgaacaa
      181 atagatgaag ctgtttccat acttcgtaaa caataccaat aaactcatga gcacagaaaa
      241 aatcattgct gaccaaattg atcgcattct tgcggatatc aaaggggtta ccgccgtatc
      301 cgaaccttca tgcggttgcc gcatcaataa gcccgcgacg ccaagcgaag cctgcgcatg
      361 caataccgca acggaagccg aaatctataa caatttcgtc gaatgcggtg cgtgtcgcat
      421 ttccgctgcg ctcggcattg atgccgaaaa agaacgtgtc gggcgaatga ttgatcatac
      481 gttgctcaag cccgaagcca cagcgaaaga aattgaccaa ctctgtgaag aagcggccaa
      541 atactgtttt gcatcggtat gtatcaatcc aagctgggtc aaacgcgccg cacaaaaatt
      601 ggcgcgtagc ggcgtcaaag tgtgcaccgt cgtaggtttc cctttgggcg ctacaagcac
      661 acaggccaaa gctttagaaa cggaaatcgc cgtatcgcac ggtgcacaag aagtggatat
      721 ggtgatcaat gtcggttggc tcaaatccgg tgaatacgaa ttggtcaaaa aagatatcgc
      781 tgctgtagtt cgtcatgccg gtcgccaagt gcccgtcaaa gtgattttag aaacatgcct
      841 tctcaccaat gaagaaaaaa taaaagcctg cgtactttcc aaagaagccg gcgccgctta
      901 cgttaaaact tcgaccggtt tcaacaaagg aggcgctacc gcggccgaca ttgctctgat
      961 gcggcatgtc gtcggacaag acatgggcgt caaagcatcc ggcggcgttc gtacgtacga
     1021 agatgcgctg caaatggttc aatcgggcgc atcacgtatc ggcgcaagcg ccagcgtaaa
     1081 aatcgttacc ggcaaatccg gcgaagcagc cggttattga ttgatatgac gattaaaata
     1141 tatcttaatt catcaggact tcaacatgcc taatcgtttt aaaaccattg agcgacatat
     1201 cattgacatg gaacgcctgc accccgaggc atcgggtgaa ttgacaggtc tgctttccga
     1261 cattgcgctc gcgggtaaaa tcatttcatc agaagtcaat cgtgccggat tagtaaacct
     1321 gctcggactg gctggcggcg aaaatgttca cggcgaagcg gtaaaaaaat tagatatgtt
     1381 ttctcacgac atgctggtca aaattatttc gggcggacgg ggcgaaattt ttatgctggg
     1441 ttccgaagag atggaacatc ctctttcact tgatgataat tatcccgacg gcaagtatat
     1501 cgtcaatttt gatccgcttg acggttcatc caatattgat gccaatgtca gcgtgggcac
     1561 gattttttct attctaaaaa aagactcatc aaccaatcct aaagaagcct gccgccaaag
     1621 tggtgtccag caagtcgccg caggttatat tatttacggt tccagtacga tgtttgttta
     1681 cacggccggt catggtgtac acggtttcac actggacccg gggctcggcg aatttatact
     1741 ctcgcatgaa aatataaaaa cacccgaacg cggaaaaata tacagcatca atgaaggcaa
     1801 ttcgaacaag tggcacgacg gtattcggaa ttacatcgca tatcttaaag aagaaaataa
     1861 atccgataaa cgtccctatt cctcgcgtta tatcggatct ttggttgccg attttcatcg
     1921 caatttactc tatggtggta ttttccttta tccggcggat cgttccaatc ctgagggaaa
     1981 attgcgttta ttatacgaag ccaatccttt agccatgatc gttgaagcag ccggaggagc
     2041 cgcttccgac gggcatcggc gcatcatgga aatcgaaccc acggctttac atcaacgtgt
     2101 tcctcttatc attggaagta aagcggacgt agaaacagcg tgtgctttta ttcaaggtca
     2161 gcgttaatcg tcaattctgt tttatgatga ttggattaaa ggccttggaa accacggtaa
     2221 cgcgatcacc gatttgaagt gattcgatat ccgaagccgt tgctacaacc cgaatcaatc
     2281 gctcccctga tgcgatcgtg agaataaata tcgtatcatt gggctccatg cctacgactt
     2341 caccatccat tctaaacttt ccgctcaaat gtttatcgtg ataaacatcc atcggattac
     2401 ccagcttgac cattcgccca ttatccaaaa cgcaaacccg atttgataaa cggcaaattt
     2461 ccgccatgtc atggcttacc caaaccatca tcgaaggcgc ttcattccac agagttaata
     2521 tctcttgctg catttttttt cgcaacgcca catccattgc ggcaagcggt tcgtccatca
     2581 ggagaagcac cggcttacga atcaatgctc gcgccaaagc aacacgttgc ttttgaccgc
     2641 ccgacaacga atcaggtctt cgcgaagcaa gaccctccag ttcggtgagt tgtaacattc
     2701 gccggaccgc ttcatcatcg ggttcactca acgcaaatgc gaggttttca tataccgtca
     2761 tattgggaaa caatgcataa tcctgaaata caaatcctac ggcgcgtcgt tcggggggac
     2821 gattgatttt tttttcggaa tcaaaccacg gttgatcctg ccaataaatg cgaccgctat
     2881 ccggttcagt gagtccggcc agcatacgaa gaagggttgt ttttccggag ccggacacgc
     2941 cggttaatgc taaacgttca tgcgcgggaa tctccataca tatttccata acgacatgcc
     3001 cggaggatgt ttcaatcgtt tttttaatat caatggatag cattatcggc ctctggcatt
     3061 atttttggac tcgataacat gaatgccgaa taacacacac cacgaaaaaa ttactaacac
     3121 aagagcatat gtgtgagcca tcgaataatt catctgctcg acggcttcga atagcgcgat
     3181 agaagccact tttgtttttt caggaatact tccgccaatc atgagtacaa cgccaaattc
     3241 gccaaccgta tgcgcaaacg tcatgataga cgccgaaacg acagccggac gcatattggg
     3301 taatagaaca cgatatagta tctcccctct tgattttccc aatgtttgtg ccgcttcgat
     3361 caacgatcgg tgcactgaac caaaacccgt acgcaacgga ttaaccataa acggtaaact
     3421 gaaaataaac gaaccgataa gaagtcccgt aaatgaaaag gcaaggcgca catcaaacca
     3481 tgattcgatc gcagcgccaa accaaaattt cggtgaaaac gcgatcagca aataaaatcc
     3541 taataccgat ggaggcaata ccagaggcat gcttaccaac gtttgcaacg gtatgcgcca
     3601 tttgctttta gtaaatgcaa gatagtaagc caacggaata ccggctatca gcaataatac
     3661 cgtcgtacaa cctgccaatt tgaacgtcaa ccagaaaggc gcccaatcca tcatgctacg
     3721 cgtcctccca aacagcaacc gcttgcgcat tcacgtatac ttcaagatta tgattttcct
     3781 ccaacgacat acgcttggcg ttttctgtag tgagaaccga tcggatagta cctatcgcag
     3841 tttgtactcc tacttctgtc aaaagtttac cccatcgaat ttgaacaatt gttcctgtcc
     3901 attgattgga catactaccg tgccggcagt cttgtggtaa taaattcact tcggtttctt
     3961 taaacgcagc atacacttgc acacccggtt ttagatacga tgccgtctca ggtgtttcga
     4021 taataacggc tgttaacgga gcgttcgcgc tttgcatcgt caccaaagat agtgatgctt
     4081 cggtttcaat ggcaacgata tggcatttaa ttttattcat gaattcttgt ataaccaaat
     4141 ttctcaaaaa cttctttggc tgtatccgaa taaataaact caaagcattg ccgggcctcc
     4201 gacttatcat ttaataaagc agcgctttgt atgatcggat tatacagctt cgaatcaacg
     4261 acacgataat atcctttcaa actcgtcggt gcactcaata ctgatgacaa cgctgtcaac
     4321 cctatatccg ccgttccggt tgtaatgtac tgattcactt gggatatatt ttcaccgaaa
     4381 atcagtttat ccttcacgcg ttcgtactga ccggattttt tcatcgcgtc catggcggcc
     4441 ttaccgtatg gcgccaatgc cgggtcagcg atagcaatat gccgaatttc ggcttggtct
     4501 atagaaccca tcgcatcgtc ggagtaaagc ggcctagcag accaagcaac caattgacct
     4561 tgggcatata aacgcgggcg catacgcgtc aacttctttt cgtataaaat attgggaaat
     4621 atcgtatcag cggataaaaa aatatcatac ggcgcgccgt gttcgatctg agtcgcaagt
     4681 ttacccgatg cgccggaaat gatctctacg cgaattccgg tttgttgcgt gaatatcgcc
     4741 atcaaagtct tagttgcggg acttacattg ctagcgacgg caatacgtac cgacgactgt
     4801 gaatgtaatt cgaagacatt caataccatc aacatacaca aattggcaaa gagatttttg
     4861 atcaaatgct gtaacgaagt aggcattcgt taattacttt ttaaaatcat tgatgatgat
     4921 aacgctcatt aaggctaatc taaaattaca aatttacgag attaatcaca accatagctt
     4981 atttatcctc gcagctcaag ttattgaaat tttaacgctt atgatgttcc ctcgaaaatg
     5041 aaattgtaaa aatttatgtc attatacccg tgaatagtcg gtttcccttg cgaacactaa
     5101 aagtgcgtcg tatatttaaa tgaagacatt acttcacacg catgaccact tcgcctattt
     5161 taatacaaaa acgctttcaa tttcctccgg gtcaaaatcc cttcataacc gaagaacaac
     5221 gttttttttc atacaatcat tacattcgtt cgcgtttcgg tcaaaggatt catcgtgtta
     5281 cggtggatgc cggatttaca tgccccaatc gcgacggaac ggtggcttcc ggcggatgta
     5341 catattgtaa caacgacggt ttcagcccgg cttcgcagat ttatcgtgta aaaaaatacc
     5401 ttaaaccttc aacgccggta cacgatcaga taaccaacca tattgatgca atgaaaaagc
     5461 gttttaaaac ggataagttt ttcgcgtatt ttcaggccta tagcaatacg tatgcgccgg
     5521 tggacgaact cgaaacgctt taccgagaag cccttcatca tccggatatt atcggtcttg
     5581 tcgtcgggac acgtcccgat tgtgtggacg aagaaaaact ccagctttta cagtccatag
     5641 ccgaaactca ttacgtcagt atcgaatacg gttgtgaatc catatacgat aaaacattac
     5701 aatgggtaaa tcgcggacat gattttgcct gctttataga cgcggtcgaa atgagcgcgg
     5761 gacgtaattt ggatgtggcc gcacatatta ttctgggttt tccgaccgaa acacgggacg
     5821 agatgcttgc gatggccggt gcaataaatc gcttaccggt agattttata aagattcaca
     5881 acctccacat tgttgcccaa acaccgctcg cctttcaata caaaaagtca cctttccctg
     5941 tgttcgagca acatgaatac agcatgttag tatgtgattt tcttgaaaga ttgtctccta
     6001 acatttgcat tcaaaggtta tatggcgatg ccccgcgcgc tatgcaaatc gctccccggt
     6061 ggtccacaac cggccaggat atgaatcttt gggttcaggc cgagttgaca cgtcgtcagt
     6121 cgtttcaggg tattcgtgca actgaatgat cgtctgaatt atttgttctt tgctacgcta
     6181 cttaccactg cgttacggta tactctgtaa cgccaaaacc gtaggaggtc tccatgataa
     6241 gccaaaaaac gatcgaacgg ttatctaaaa tcggcagcag agattttttt atcaccagtt
     6301 tatatctcaa tttggatcat acacagaaac acggccaata caaaatcgct ctgaaagatc
     6361 tgctgaaaga ccgtcgccat cgtctggatg aatttcgtaa gcataacaaa ataactaagg
     6421 aacagatcaa atcgctcgaa gatgattttg ttcgaattga aaattatatc cagcacgaat
     6481 ttgtcagcaa agattccgga aaagggttgg ccattttcag ctgcagcgcc acgcatttct
     6541 gggaaattct cgaattgcct caaccggttc ccaatcacct caatgccgat tttgatccca
     6601 atatccgcat tttgagcgaa ttattatgcg attaccgtca ctatgccatc gctctcgttg
     6661 atagcagcaa agctaaaatc gttgacgtca atctgggttt cgttcgtgaa gtatggtctg
     6721 tcgaacatgc gataacaggc aaaaccaaat ttggcggcgt ggatggcacc caagagcgca
     6781 atgccgagcg agcgcacgat gaatccgttc ataaacactt taaggttgtg gcgaccgagg
     6841 ttgaaaaagt cttcgaccac agcgaccata tgtggcttat cctcggcggg cgacaaaatt
     6901 ttctaccgca gttcgagtcc cttctcccag ccccggttcg taaaaaaatt gtcggccaca
     6961 tcgtgattga tccggaggca tcgctcaacg aagttttggg caaagcgtac actatcgccg
     7021 aagaagccga agccgctcat gaaaaagaaa ttattgaaca attgcgcggc gaagccagta
     7081 acggaggtaa aggaattttt ggcttacaac ctactctgca aagcctccgc aaaggcgggg
     7141 taagcacgct tgtagttacg aaaggatatc aggcgccggg gttcgtctgt cataaatgtt
     7201 tttttatagg cacacctgag gaaaaaggca aacacgatac ttgcccgatc tgttccggac
     7261 cggcacacaa tgtcgaagat gtcgtcgaag aagccattac ttttgcttac atgcaaggat
     7321 gtaaagtaga aaccaccaat gaaaattcgc gcatgcgtat catgggtgac tttggagcgc
     7381 tacttaggta ttgacctcat gatgagtttg ttatatgtta caaaaggtaa gctaaacggc
     7441 ttaccttttt tttaacaaac acgatatact atgaccacca catgctctgt atttcttacg
     7501 atctttgtcc tgtgtttcgc gcaagcccaa agtagcaacg gtcaaaattt agatcacatc
     7561 gaaaaattca acagcgtaaa atccctattt acatcgcgat gcatttcttg ccacggtggt
     7621 gatgaaccgg cggccggtct gcggcttgat cgaaatctct atgcgcagat cgtcaatgtt
     7681 cccagtaacg gcaatcccga cttacatctc attcagccca actctccgga atccagttac
     7741 ctctatcgaa aagtagcaag acaaccaaac gaattacctt ttgcggatga tcccatgccg
     7801 ctggacgata ttccgttaag ttcttccgaa ctgcaaatca tacgtgaatg gatcctctca
     7861 tttgatccgg ccttatggcc tgcggaaaat caaaataaca cgattattca aaaccctgct
     7921 acagagatcg taccaaagtt tctggctaca caattgatca atatgccttc gacgcaaact
     7981 ttgggtaacc gtagtgcaga atttcgcatc atgcaccgtt ttggcttgat caacggcggt
     8041 cccgacggtg gaaatacgta tggaggcttt tttggcatgg atgcgggtgc tataacgagt
     8101 atcaattttt ccatagcttt gacaaataat gcggatattc tgcttcgacg tacaggaacc
     8161 aataaagatt ttgaattggg cgtcaaatac gtaccgtttc attttaacga acgttttccg
     8221 acggatgtcg gtttttttgg aagcatagac tggattagcc gagatgacgt gcccgcgcgt
     8281 cataaaattt cacctaatct ccaaatgatg atcggtagcc gcccgtttga gaacctgagc
     8341 atactcatag cgcccggaat cgcgtttaaa agtaaccaca atgctacaat caccaagacg
     8401 gtcaacgata ctacaaccca agcctatcgc gatacacggg ctactacggc tataggactg
     8461 ggggcggaat atctgatagg ccgaggctat gcgatctcag gtgaatacat tcctcgtctc
     8521 ggcggctata aaggaaattc gttttccaac gatcgcaggt atgataattg gagccttgct
     8581 ttttcctaca aagttcgcct ccacgtattt cagcttttag tttccaatac ccaaacgatg
     8641 cataccactc aatatttgcc cggaagttca actattacca aaaatgcatt atacaataag
     8701 ggttggaatt ttcacttcgg gtttaacata tacaggcaat tcaaatggta aaagaaatca
     8761 aattattttc gttactcgtt tttgtaatga ttttattatc ggcatgcggc gaattgaaag
     8821 aggaatctca gccgacgtta ttagtaccgg cagatgtgca ggctatattt aacgatcaat
     8881 gtaccggttg ccactctggc tcgtcccctg caggatcgct taatttgtcc gtgggtaacg
     8941 cgtatggcca attggtgaat gtcacaagta ctattgagcc tgcattaaag cgcgtcgtac
     9001 cgtcggatac agcaaatagc tatcttgtgg ataaaattat gggcacacaa acagttggag
     9061 aacgtatgcc ggatggcggg ccctatttgt cggcagctca aatgaacatt atcaaattgt
     9121 ggattcaagc cggtgcggaa gccaaacctt aattgcatca gtcggctgta ggcgaaaatc
     9181 aaaaaaataa ttctgtttat ttcattagaa ctgttaccac ggtccaatat ttgaccggta
     9241 cgccaccgga taatccctgc tcgaataacc attgcttcac agcagcgatc gcggcgtcgt
     9301 tgatcatttg gtcccagtct ttgttaccaa tgcgtttgga aacgtaaatt tcttcaggtt
     9361 ttccatctgt tcccacaagt acgcgcatcg aaacagttcc attcagctta gcctttttag
     9421 caagatcagg catgaccggg ctgacttttt gaagcgcttt aggattatta tctaactcat
     9481 tggccatctt tgtgggtaca aaaaccggtg ctgcaggttt agattctaca ggcttgggcg
     9541 tggttcctgc ggaagcggcg ttagtcgtgg acgcctccgg agtaggggtc gttgtggctg
     9601 cagcggtttc tttatttacg gtcacgacag gctgttttac ggtagccgca gccgccgttt
     9661 tcttttgatc ttcttcctgc tgcttttgta atgcaagtcg gcgttcttgc tcaatacgaa
     9721 tctgctcctg ctgtttgagc tgttcttcaa gtaaagctaa acgctctttt tccttacgtg
     9781 catcttcggc gcgtttttgc cgctccgatt cggttaaccc tgccccaccg gctttgtcgg
     9841 tcggtttggc ctgcgccaat tgtcgtttta aatcttcgat ttctttgcga cgtttttctt
     9901 gctctgcttt agtatccgtc ggcgcatcgg ccgcgatgtt tttaaattcc agataagata
     9961 tgttacctgt gctgtacacc ggggtaaata cgacgagacc gaatttgtta ttctcatcat
    10021 cgtcatggat aaagcgatcg gcgttaaaat tatcacccca gtcgttacgt tgtgcgtaaa
    10081 cgacattttt ggtatggttg tatagattaa actccaaatt attataaata gcatttttcc
    10141 cgctattttc gccaaggcgc gtatcgccga tcagcggttt agagacgtcc tgtactaaaa
    10201 caccataacg attgcccgta atttcattgg cgttaatgct tggcgctgaa tttattttgg
    10261 cattgatacc ggcgtaaccg ttattctgta tctgattgtt gttaatttta ggtgtggaca
    10321 taatatcgca aaggatacca ttccaataat ttccttctat gcggtttcga aatatacgcg
    10381 gcgaggaatt gatgcaagtg ataccgtcgt aagcaaattc aatcacgcag ttcgataaaa
    10441 tactgcggtc ttcacctcga cgaaatatta aaccgtacca gtcgcccgcc tgaggttgaa
    10501 gtgtgcttcc tttttcgccc ggatcggtaa aattgctgga ggtaaaccgt atttgaaatt
    10561 tttcctgacc ttcggctcgt aaaatacctt ccacaatcag ttcggacttt gcaacgtcaa
    10621 tccctccggc ttcatcatcc tggttcgcca taaatcggat caacgtattg gggcgtatgc
    10681 gcacggtaat gccttccggt atcgtgacat ctccggtaac ggtaatcacg ccctcccatg
    10741 tttcgtcttt tcgaatgacg ccggatttgt ccgcagccca cactgaaaaa ccgttcacca
    10801 gtacaatcgt gagcagtatc cgcagcctgc acctatggag tttaaagaaa ttatacgcca
    10861 tgctgggttg cctcaaacat ttcatccaat aattggggtc cactcttgag caaatttttc
    10921 aaaatcgcat ctttttcaat cggtacatca tcaaaagctt ttgccggttt ttttaccgct
    10981 tttaccttgg tttccgtttt cttttctgaa attacttttt cattcggagt caccgctttt
    11041 tccggcactt tatctaactt aatctcttgt gtaagctccg ctttgatacg cgcacggacg
    11101 tcctcgtcat tgacgggttt gatatcggtg gcttcgggcg ctttttgttc ataactctga
    11161 agttttccca cgacatcgtc cacatcaaca tccgtaccgg ccgtaagctg tttaaggcgt
    11221 tcaatactcg tctcgtaaag gtcaatcagt tccgatgtga cacgtaattc accctcattc
    11281 attttttcgg cgaccgtttc catcggtttg acgaggttac ttatcttttg gaaacccagc
    11341 atttttgccg aactgcgaag gctatgcgcc gctttttcga tgtcgcgcat ggattgccga
    11401 tgcgtcatat tatttcgcag cgtactcagc gcagctccga taagcttgaa gtaggtggat
    11461 acttcttctt taaagatatc aacgatctcg tcgtcaattc caagtttcga aaagtaattt
    11521 tctttgggag caggttgaga aacctcggcc ggtttgacag cttcgataac aggttcgggt
    11581 ttcttctccg gcgctttagt ttcaatgccc ttatgtttgg ctaatgatgc gctgccggga
    11641 atggcgcctg taaaagtacc tgaggcatta agcgaatttt ccagttcaaa caactgctgt
    11701 actacctgcg ttacatccgc cgcttttcgg gccgcgatca aacgcagagc tgtagtgata
    11761 gactccaata acgtgactat cgtcgcatca accgatattt ctttttcaac acaccgttcc
    11821 gtgatgagtt ctacggccgc cgataaaccg ctgatacgtt caaatccaag catacgcgcc
    11881 gaactcttga gactatggct ggtgcgctcc agatcgcgaa tggccacatc gtcaactaaa
    11941 gattgcttca gacgggcgaa cgccgcatca aactttttga aatattcagc ggcctcttga
    12001 ttaaaaatat ccaaaatttc aggatccacc gcatcaaagg ccgattcttc caacatcagt
    12061 tttccttcga caaccgccga ttcgactttt tcggttttca aaggcggcgc taccgtcatt
    12121 gtcttgagcg cttccttgac cgatgctttt tcgggaatag gcttcacctg ttctttggga
    12181 agctcgcttc tttttttctt gacggtacgg cgaccgcccg tgatgatatc tttgggttcc
    12241 gtattaaggc gttcggcaaa agaagggata cgcaacccgt tcaattcatc atcggcatca
    12301 ccggcggcca tcatagctaa taaggctttg ggaacttcga tttctatcgt cttagtatca
    12361 tccgtgaccg gcgaagccag tgtggactgg cgatcatctg tttttttatc aggcgttgct
    12421 ttttttatct cttcgaccgg ttttacttcg atcttgtttt gtacaacggc ttcagcgaca
    12481 aatttaggtt tctcttcaac ccccggcaaa atagtctttg taggcgttac actttgtgta
    12541 cgcgtttctt ctttgatttg taaagtcgaa tccttggtgt ctttttcatt gggttcaact
    12601 ttttccgctt tgatatgttg cgcaatcgga gaatcgttaa attttgcaac gaccgactga
    12661 aatccctcaa gccagtccgt gttttgtgct actacggact tcggatcgga tatcggtttt
    12721 gaagactctt tcgccgtttt tatttccgtt tttacaactt ctggcgcaac tacttttgtt
    12781 tgctcagatg gtacggcgct tatctcagtc gttttagcgg gtgttactaa tgtcgtcgct
    12841 tgtttataat ctgaaaaacg aaccgattga tcattgagcc atcgccattc atcagactgg
    12901 atcgtttgcc cgttgaccaa aatttcgata ctacgcgtcg atcgcgtcac aagggtgatc
    12961 aattctgcga cgactgggct ttgcgaataa actacttgtt tgaaaatctg gaagaccgcg
    13021 tcacagtgcc cgtgtagtaa attgattttt aataaccgcg tattggttga cagtgcacga
    13081 tatacctgca aaaggtcttt gatggcttcg gtgtcgtgcg tagctttttt gaggcggacc
    13141 aacgccgact gtgccaattc caaattaaac cgcacatctt cggcaaatac attccaatca
    13201 tcgtcttgta tcgcaacacg atcaatagcg atttcatttt cagctacaag ctgtgcattt
    13261 gaacttgacg actgaaccgg cgattcttca ggtttttcca tattaaacga accggtaaac
    13321 cgttgctgta gcctcaaaat ttctgccggt gcaagcgatt ttaagtctga attgttgttt
    13381 tcgacctgtt gaatcaaatc gtgcaacacc gccaaggcct ccggattcag aggtttgtga
    13441 ttattatccg caatcgcatt cagcgtttct tcgatccgct gggtcgcctc ggacaatggc
    13501 attgcatttt gtgtgacggc ctccgctcta agttgcgaca gaccatcggc catgctaatc
    13561 agaaatcgag ggcgtgtcgg attatcccgc aacgtctccc atgcgctatt caatcggtcc
    13621 gttgaaaaaa ccgaccggac tgcatttata ttttctttcg ggtcaacttt ttcggttggt
    13681 tgaacaattt gtgcaaccgc tttttcaggt tgtttttttg ttcgccgtga caagaatccg
    13741 atcatatcgc ccacatgatc aaacgcttcg gattgcgtta taggcgcaac ttccagtagc
    13801 gaagattgcg gtgatgcgga cgtcaccgat gttttttcat gggtagattc tgcacgggag
    13861 gtattgtgag aaaaaatgga atcgtttttt atcgccggtg aagctggggg ttcagttttt
    13921 acgccaacgg atatcggttt gacctcatcc ttgggcgttc cttccgatgg agacggcgta
    13981 tcttcttgct tgatatacgt ttttcgtaag gccggcaaat caagcgaaaa gccgatggtt
    14041 tgtaaatcgt tattgagttg atcttgtgaa ccgggtgttt ctaaaatttt ttgaaacatc
    14101 tgaatgatcc gttcggaatc attttcaagc gtcgtgatca tctggctcat cttgtagccg
    14161 gatttttcta atttctccaa gttactacgc aaaaaggtgg caaccggatg gagcgatgcc
    14221 acgttgagaa atgattcgac ctgacgaacc ggctcttgcg cttgtccgat ggatacgatt
    14281 atttttttgg taatactgaa ttcaagcggc gcaaaacgat gttcggaaac acttttgaca
    14341 tgattcagta attccgaaag ataaaattct acgatttttc gcgatttatt gtccaatgtg
    14401 tatcatccat acaaaaaaac cctaaaacac gtttagggtt tttacgattt acgcgatgct
    14461 atggcgttta tttaccgaga tattcgtgta ctttggcaag caagtcttca tcatggaacg
    14521 gtttattgag gaaatccgtc gcgccgttgc tgatcgcggc gtctttatct tgcggactgt
    14581 ctttaccgct caacacgacg atcggcaccg tggcaaaata tccgcccgtt tcacgcaacg
    14641 ctttcatcac ttcaacaccg gtcatatcag gcaacatcat atccaaaaga atcagactgg
    14701 gctgcatttc tttagctttg ttgatcgcat cttcgccttt catggccgac gtcagattgt
    14761 aattatcatt ttccaacgca atctcaacca ttttttgaat cacgatactg tcatccacga
    14821 caagtatctt tcgaccgccg gccgacgcag aagcggccgg agctaccggt ttgggcgccg
    14881 ctttttctac tttaggttcc gacttgggtt cgaccttagg cgtatccatg gaacccaaaa
    14941 tgtcggtttc ttctttttta ggcgcaggtt tgggttccgg tgctttttca attttcggct
    15001 ccggtttggg ttctgcttta ggttccggct tcggttccgc tttcgcttta ggcgcgtctt
    15061 ctttgacggc tttcttcgac ggcgctttct cttttgccgg tttagccgcc ggagcaggcg
    15121 caccgccgga tatcaatttt tcagcagata gtttttgtaa aatttcttcg accaacgttg
    15181 catcgcgatc cagtttttct gcgatatcgg ccacattgcg tttaccgtca acagaaaaca
    15241 aaacacgcca ttcaattttt ttcaacagaa tggtctcaac tttttctcgt aagtctttta
    15301 ctctggagta aactgcatcc acaatatacc tcgccgcttt atgatgatga acctgtgagt
    15361 attcaaatcg tgttaataac tttgtaaatc taataaagat aaatcattat gtcaataagt
    15421 ttcaatgtct tttctctcgt tttctgaaaa aagaaatcag cgcttcggca taatcactac
    15481 ccgtaaaaat attgatcaaa tcaacgcgac tgcttttgaa aatttccttc aatctgtttt
    15541 cttcggcatc aacgcgctgt cgcaatatcg catgaaactg ttgatcggcc gtgtcaatat
    15601 caatcaacgc cccggtttct tcgtcttcca gtgttaccag accgaccaga ggccaatttt
    15661 gctcgcgctg atcggcgacc cgaagaccga tcacgtcgtg ctttttagct aaggtacgca
    15721 aggaggtatc aaaaccctga tcaaggaaat cgctgatcac aaaaacgata ctgcggcgtt
    15781 tgagaatgtg tgatgtcaac tccagcggag ccgcaatgcg cgtaccgcgt gctttaggta
    15841 caaacgcgat cagctcgcgg atcagacgca acacatgaga acggcctttt ttcggcggaa
    15901 taaatttttc aacttcttcc gtataaatca ttaagcccac cttatcatta tttttgatcg
    15961 cgctaaatgc cactaccgcc gccatttcga tcatcagttc ccttttggtc tgcttattcg
    16021 taccaaagtc ccctgacgcg ctggcatcaa aaatgatcat cagcgtctgc tcgcgttctt
    16081 cttcaaatat tttaaggtac ggtttatcca accgcgccga gacattccaa tcaataaaac
    16141 ggatttcatc gccgaactga tattcgcgca cttctgaaaa agacattccg cgccctttga
    16201 aaaccgaatg gtattcaccg ctaaaaacat tattgaccaa tccgcgtgtt ttaatttcga
    16261 ttcgccgcac attgcgaagt aattctttgg tttccataaa atccgcttaa ctatgattaa
    16321 atgcgtaata cgtccgtatc atgctacaaa ttctttgagc tgaatcattc gatcaatata
    16381 aaaattcgca ccttccggtg caacagcatg aaacccataa cgaacggcgc atgtataaac
    16441 ccctgcgcga cgccccgctt cgatatcttg cggaccatcg ccgatgatca ttgcccgatc
    16501 gggatgtacg ccggtttttt ctaacatggc ctgaatagcc tgcggatgcg gtttcttatt
    16561 tgcaaacgaa tccccgccgc cgtatacccc ttcaaaatac cgggacaatc caaggccgtg
    16621 aataattttc cgagtaaaca cttccgcttt attactaatc acaaacttac gacagtgatt
    16681 aaaatgagtc aatacatcaa gaactccgtc gtacgtgcga acaaaatcaa gacaatgttc
    16741 gtcgtggtgc gtccgataaa tagatacggc cgtcatcaac tgatcgttat ccggtttctc
    16801 aaattcagat aaggcacgcg caagtaaggt ttgcatgccg tcaccgacaa agcgacgcag
    16861 tttttcttta ggtaatgcgc tatatcccaa cgttgttaat gtatgattga cagacgcgcc
    16921 gatatcatcg agtgaatcca ctaatgtccc atccagatca aaaaacatca cttcgatttt
    16981 tatcacgcga tccatccttt atacgacacg taccgccaac ccaatataaa accagacacg
    17041 atcagtaccc aaccggtaat tacagctatg cgtcgaatcg ttgtttcctt catcgtctga
    17101 cgacgtacgg caacccactc ggcgagaaga aaaaaccacg cggccgtgcc gatggccata
    17161 ccggcaataa acgccaattg agactgaagg gacgattgta aaaattgata cgcgtacata
    17221 aaacccgctg ccgcaatcca aaagccaata gccaacggat tgcataaata cattatgcat
    17281 ccgttaataa aatttttcat caatagtcca gctgaaaact cggtacgttc gtgatatacg
    17341 ttgccgttat gacgtgtgat gatcagacga tatcccaaaa aaacgagcac cggcatgccg
    17401 gctggctgta acatatcgat caatgattgt tgtaaagcgg acataccgaa aacaccgatg
    17461 atacagtaac cgatatccac aaccagtgcg cccaagccga tcatcatagc cggctgaaag
    17521 ccgcggcgaa aactttgatc cacgatagtc atattgatcg ggccgaatgg aatggaattg
    17581 ataaaaccaa tacctaaacc ccacaaactc gccgtgatta tatttcccat cgtcatcatc
    17641 gctgccaccg tgcgctgatc caaatatccg agtccagtat ccgaacccgg ggctcggcta
    17701 aaacaacagc attattaata cgtttgacgc gtaggccgtc aaaagatgat ttcccatgtc
    17761 ccaaaatttt agcggccgta aaaacatgta tttcatcgca caatcccgca ttcaaaatct
    17821 gtccatggat atccgctccg ccttcgacga ataccgacgc aacttgaaat ttccgcagaa
    17881 gcgttctaaa tatggaacgc atggctatcc gatttcgcac ggaagacatc tcaacgaccg
    17941 ttacgcccaa cgatgccaac tgcctttttt tgtgtttgtt cgctcctaaa gtcgtaatga
    18001 caatggtttc cgaagcgccg gcatcggtga acacccgcgc tgtcaaaggt gagcgcagcc
    18061 ggccatccac gatgacgcga cgcggcgaag acccgtgaac gccgcgtacc gtgagttgcg
    18121 gatcgtccgc catcacggtc tgtgcaccga ccaaaatcgc atcgtgccct gcacgtaagc
    18181 ggtgcacttc gcgtcgggat ttttcattgg ttatccactt ggaagcgccg aaaccatcgg
    18241 ccgtttttcc gtccaaagtc tgcgccgttt ttatagtgat ccacggcaaa cctgtttcca
    18301 cgtgcttcaa aaaaaaccga ttttcaatac ggcattcact ctcggctatg ccccactcca
    18361 cacgaactcc gtttttcttt agaagcgcgg cgccttttcc tgccacagcc ggaaacggat
    18421 ctttgatccc aaatacgaca cgtggaattt tttcttgtaa gatcaaagac gtgcacggcg
    18481 gtgtttttcc cgtatgacag cagggctcca agttaacata caatatggta tttttaaaat
    18541 cggtcacccc gcgacgctgc gcatcacgta cggcttcgac ttcggcgtgc ggctttccgt
    18601 accccgtatg ataacctcgt ccgataattt gatcaccgtt tggggtatgg cgcaccaaaa
    18661 tggcaccgac gagcgggttc gttttggttt tacctacgcc tttacgcgcc agtttcaacg
    18721 tctgccggat ataatattcg tgtgtctttg gagatggcat aacatgcttt ttattaaaat
    18781 cgtatttcaa tattactcaa tcgccctgct ctgttcaagg gtataaaaat tcaatccctg
    18841 caattaccat tgcgcttgac aatgggcgtt cggaagtgta ctttggcgaa gtttcaatta
    18901 tttttgaaag tttaaaacta acaaaacatg cataaagcgc tgcataaatc gcaagaactg
    18961 tttacggcaa cgtggggtga aattagcacc ttttggggta tcaataaagt aatgggtcag
    19021 ctttacgcat tactttttgc cagcgctaaa ccgctcacgt tggacgatat gtgcgaacgc
    19081 ctgcaaatca gccgcggtaa tgccagtatg aatatacgcg cgctcaaaac atggggcgtt
    19141 atccgcaaag taagggtgca aggcgaccgt aaagattatt acgaattgga tgaagatacg
    19201 ggtaaaatta tgacccggtt tgtgcgcgag cgaaaacgac gcgaactcga accggcatta
    19261 gaaacgatgc gtcgcagcgc catattgctt cgcacggcca ttcccgctat gaaaaacgga
    19321 gaacgcaagc aggcgcagat ctttctcaag cgtatcgaaa acatgcttat ggtctccgaa
    19381 agcatcaata ccctgctgga acgctttatt catggcgagt ccatcagcat caaccaatta
    19441 aaaaccatac ccatagcttg gaaaaaaggc gcataacaca taaaggtgtc gtatgaatat
    19501 catcgaacgc gaaccgtgga tacaaagttt tttacaagaa accgacgcgg tcatccacga
    19561 agtttttgaa aatacgggta gcgctgaatt taaggatatc ctcaaaaccc atcatcaggg
    19621 cggcaaacgc cttcggccga tggtcattgc ggcttccgct caactcggtg atcgcccggc
    19681 gcaccatctc gcttacggcg cggctgcact cgaattgctc cacttagctt cattatttca
    19741 cgacgacgtg cttgacgata ccaccatgcg tcgcgataaa ctgaccgcgc gtaaacatat
    19801 cggaaatctc aattcgatct taaccggcga ttacctgctg gccgaagttt tgcatacgat
    19861 cgtcgaaaaa ttacctacgc cccttgtcag cgcgtttctt gatacgatca aacgtatgac
    19921 acgaagtgaa attttatcca aaaaacttct gaacaatttg cagatcagta cgtctgaata
    19981 cgttcagatt attgagaaca aaactgcaac ggtttttgct ttggcgactt ctctcggtat
    20041 tcgcctgagc accgatgacc cggtgcgcat tcgtcagctc agtatctacg ggatgaacct
    20101 cggtatggcc tatcagatca ttgacgacat ggaagatatg accggcgctt cggaattaac
    20161 cgatgatgat ctttctcacg gttatatcgg catgccggtg atcgagttgc tgcgtgcggt
    20221 gcccctcaat caaaaacaag aggtgctgca aatggtacgc gccacccagc ccgaacaacg
    20281 tgctgcgttg attcgtatga tgaaaagttt ttccattttt aaagtcgtgc atgcccgcgt
    20341 acaacattac ctcaacattt cggccaatgc gcttttggat attaaagcca acacgccgga
    20401 gcaacggatt tctttggaat ttttgaaagg cttgtgcgga tacttggccg ataaatcact
    20461 aaaggctgtg gatacgtacg agtcagcgga tcacaccggc acacaaacag tttcggcctc
    20521 gacaaccgta gcggctacgg cttagaagga acaacctcct ccatccatcg cagataggcc
    20581 tcatcgcctt tcgtcagagg aacggcgata atttcaggaa ccttgtagct gtgcagtgat
    20641 tttatttttt tggctaacgc ttcgaaacaa tcttcaaacg atttgattaa aagtaaaact
    20701 tccttttcgt cgcaaagttt tccttcccac atatatattg atcgaatccc gggacatata
    20761 ttacaacacg ccgccagttg ctccgtgata agcgcatcgg ctatatgctt tgcctctgcg
    20821 tccgacgatg ccgtcatata tacgatgatc gccgtgcgag ccgttacatt actcgtaaca
    20881 ttactcatag tatgaacgcg ttgtataatc gaataaattg tggattattc atcgccgatt
    20941 tttaggacgg caagaaacgc ttcttgagga atttccactt ttccgacttg tttcatacgt
    21001 tttttacctt ctttctggcg ctcaagcagt ttgcgtttac gggaaatatc accgccgtaa
    21061 cacttggccg tcacatcttt acgcattgcg cgtatctggt cgcgcgcaat gattttactg
    21121 ccgatcgcag cctgaatcac cacttcaaac atttggcggg ggatcaattc cttaagtttt
    21181 tcgcacattt tgcgtccaaa ctcgtaagct ttatcacggt gaatgatacc ggaaaacgca
    21241 tccaccggat cgctgttgat cagaatatcc agtttgacca attcgccttc tttataccct
    21301 atgtattcgt aatcaaacga ggcataccct ttggtacagg attttagttt atcataaaaa
    21361 tcaaatacga tctcggctaa aggaaactca aacgtcagat ccacgcgggt cggagaaata
    21421 taattggtat tacggtacac accccggcgt tccatcgcga cgctcatgat attaccgata
    21481 tattccgtcg gcgtgatgat ttgtgctttg atatacggct cttcgatgtg atcaataatt
    21541 ccgggcgaag gcatcatcga aggattagac acttcgatca cttccccgtt cgttttatac
    21601 acctgataac gcacgctggg taccgtcgtg atcatgctga tgttaaattc gcgttgcagg
    21661 cgttcttgca caatctccat atgcaaaaga cccaagaaac cgcaacggaa accaaaaccc
    21721 aaagccgtgg acgactccgg ttcgtagatc agcgacgaat cattgagctt gagtttttcc
    21781 aaagaagtcc gcagttcttc aaaatcttcg cttacggtag gataaatacc gctaaacacc
    21841 atcggcttga tatctttgta tccctcaaga gccgccggtg cgggatttcc ggcatcggtg
    21901 accgtatcgc ccacgcggat gtgcgtaatc tcacgcacgt tgccgacgac atatcctact
    21961 tcaccgacag aaattttatc cgctttgatt ttgtccatcc ggaggtaacc gatctcgtcg
    22021 gcatcatacc ggcagtcgtt gctaaaaaac ttcatatgcg taccggtgac cagttcgccc
    22081 tgaaacacac gtacatacgc gatagctccg cgaaaaggat cgaacaccga atcaaaaatc
    22141 aacgcttgca atggtttggt cggatcgccg accggggcgg gaatactctc tacgactttt
    22201 tcgagaatat cttcgatacc gatacccgct ttagcgctgg ctaaaacaat atcttcacgt
    22261 ttgcaaccga tcagatcaat aatctctttg caggtctctt ccggttgtgc gctcggcaaa
    22321 tcaattttat ttacgacagg aacgatttgc aatccggcat caatggcaag atacagatta
    22381 ctgatcgtct gcgcttcgat accctgggaa gcatccacca caagtatggc cccttcgcac
    22441 gcagccaaac tgcgggacac ttcataagta aagtccacat gccccggggt gtcaatcaga
    22501 ttgagtacat acgtttgccc gttgcgtgct ttgtactcca tgcgtatggc gtgcgctttg
    22561 atcgtaatgc cgcgttcttt ttccagatcc atattatcca ggacttgggc ttccatatcg
    22621 cgtttggata tggttttagt atattccaaa aggcgatccg ctaaagttga cttgccgtgg
    22681 tcaatgtggg ccacaatgca aaaatttctg attttctctg tactcatatt tccttttaag
    22741 tatttcttaa aacgcgcgca atatagagat tcacatatgt aaaatcaatt tctttcggtt
    22801 gtattattgg attgaacgca cacggtaatt attctatatt gagcccgatc ataccaatca
    22861 cgttgcacac gatttgacca tgactgtact tcctatttta agccatcaca cccgaaaatc
    22921 ctttttatgc ctgagcttat ccctaggcct ggcatacggc tcggttgcgg ggtacgcgca
    22981 agaatcgtcc gaaacggcgt ttatgcatgc cgtccgcagc taccaaaata aaaactgggc
    23041 agaaagccgc gataaactat cgtcgtatct tgcacaatat cccgacaaac ctgccacacc
    23101 ggcggcacaa attttattgg cacgcaccta tctccgtttg gataacccgc gtaaagcgct
    23161 tcaatttata aaccaataca gtgccggttt ttcttctcaa tcaccttatg cgacggaatt
    23221 tgtattcacc aaagcggaag ctttggacgt tatgcatcgc tcactcgaag cgatacagac
    23281 gttattgcct ttggcatccg gtaaaactga cgctggattg cgcgccgact cgttgatcgc
    23341 acgtttagcc gaagtgcgcc taagtaccgt ccaactcaaa gaattagcgg gatcacttcc
    23401 ttcatcgccc gtcaaagaaa aattacaaat tctgtacgcg accaagttag ccgcccaaca
    23461 gcgctacacc cgcgcatcgc gcggaatgga tgtgtttttg agagaaaacc cgcgcagtac
    23521 ttattataaa aatgcacaac aagtttttcg ccgcaatcaa aacaaagaag acggccccgt
    23581 tcggatcggg ctcatcactt cgctcagcgg caccaatgcg gaagccggcc ttgcgcttaa
    23641 atcgggtatc gaattggccg tgcgcgaata caatacaaca cacagtacac cgctggaaat
    23701 cgtcacctat gatgatcgct cggatgtgtt acaaagtgtg ttagcggcgc aagccttatg
    23761 ccgcgaagga agcatcagcg tgatcatagg ccctttggaa agcgacgcca tgagcgctgc
    23821 ttccgtcgtc tgcgaggccg cacaaatacc gatcatttca ccaactgctt cgcaaacggg
    23881 cattaccggc atcggccgct ttgtgtatca ggccaatatt gatatcgaat ccagagccgc
    23941 cgctttggcc gaatacgcga taaaaaaagc cggccaccgg acgtttgcca ttctctctcc
    24001 taatgacgtg tacggcgcca catccacgaa agcgtttacg gatcgcatca cacaattggg
    24061 cggtcgcatt atcgcatcgg aatcatattt tgataatacg caggatttca aatcgcaact
    24121 cctgcgtctt cgcaaactcg gacagatcga ggaagctcgc cccgggctgg actaccgcat
    24181 aacggctaac ttgaattttc gtcagattga ttctctttac aagatgatat ttcccgagga
    24241 caccacgacg accgaatcct ccagtgatta taatatgcct tcgcgcggaa ttgacgcgtt
    24301 atttatgcct atctatgacg acgaaataaa atatatagcc cctcaaatcg cgtattataa
    24361 tatccaaaca aaatggctcg ggggtgatca ctggaatgct cttgatgaac tgcgtcggca
    24421 aaataaatac gtcaacggat gtattttcgt cggagagttt ttctccaacc ctgatgccgg
    24481 accgaccaaa cagtttatca aaaactacac cgcagcggcg aaaaaacgcc ctacaccgga
    24541 agcattctac ggatatgata tcgtcggtgt tttaggggtg attatcgaaa gaggcgctca
    24601 actgcccgat gaagtacaaa acgaactgca aaacggtatc acatacgaag gcgttcataa
    24661 tcaaatacgt tttgacaaag gtcggcgcgt caatgcatcc gtccatcttt tgcagttcaa
    24721 agataatacc attcataaaa tcgaaccgta acccgccgcc ggaaaccacg tatgcgacaa
    24781 atacgattct ttattctgct gatatttacc tccataccgc ttgccttcgc tcagagtgac
    24841 agtatcacaa tatcggaagt gatgtttagt acaagtaata cattggactt cattgaactt
    24901 tataactaca gtaagacaca aactattgat ctcgctaaat acataattac ggatggtgca
    24961 ggcaataatg actccgatcg gatcagtagt gccggtgagg gcttactttt atatccaggc
    25021 caatttgcca ttctccttga ccccgattac tttttaccgc ccaacgcttc cggacgtgtt
    25081 tacgatattt ccattccgga tactgcacgc atcgtcacaa ttgataccaa ccaaaccggc
    25141 ggctttgttc tcggaaacgg atcgtttagt aatacaaccg cagaaacaat tcacttaatc
    25201 aaggtacaat ccggaattcc gcgtgacaca ataagtcgtt atacctattc attaggcaac
    25261 aatacttcat acagcgatga aaaaatcacc ttaaataatg ataacaattc atccaattgg
    25321 acgaattcga tttacctgaa cggtacgccg ggtgcaccgc cggagacgaa tctttcgatc
    25381 aacgatcatc gcatgatctt cacaccacct gatccgctca ccggagcaag cgtgactatc
    25441 ggcgccatcg taaccaataa agccattgcc acgagcgatt catttcgtgt gctcttttat
    25501 cacgacgccg ataaaaacgg aacagcggat gtttccgaac ttctggattc ggtttcctat
    25561 aacgtgtcac taccccgcaa tacatccgac acgatttttt ttacagcgcc ttcgatagcc
    25621 tccggcatta attattatat agcccgtatc ggttcgattt atccttatgc cgataccgtt
    25681 ttatccgatc aatccctacg cgattccgta caaacgactc cggcctataa tgtatcatgg
    25741 tccgcttctt ctgttttttc accgagttca ccggccaccg gtgatactgt cacctatgcg
    25801 cgtgcgattt acaatgcagg tatcgccgac atcacccact ttgccgccta tttgtacata
    25861 gatcaaaacc ataacggcat gatcgaaacc gatgaaaagg tcgattcagc aaattataac
    25921 gattcccttc caagcggtga ttcggtggtt atttatttta cgatcccgga cgtccaacgc
    25981 ggaaaactgc atctattatt agacttaccg tccgattctt taggtccttt gccggacgaa
    26041 caaccggccg ataatcggac atccgattct gccaatatcc gtgcggccat gagcttaacc
    26101 ctcacatcgg ccagtgcact taagccgtcg tataattaca tcgccgatac catggcgata
    26161 aaagccgtag tacacaataa cggaagcaca tccgcgagcg gtttttcttt gatattgtat
    26221 cacgacgcca acaacaacgg gttgcccgac acgaacgaac gtgtggattc tgtgcgatat
    26281 acatcgtcat tggatgcggc ggctagtgat tccatcactt tgcattggct accgacgtct
    26341 gaaggcaaca tctcgctcat cgttaaactc gattcgacca cagtccaacc tctgacggat
    26401 gaagatggat cagataatac acatacgctg aatgcgctca tctatcctcg accggattcg
    26461 atcacttttt cagaaatcat gtttgatccg ctgggttccg aagattctaa tgaatttatt
    26521 gaattgtata atttcggtac acacgctgtt gatctgctca attggaaaat cgctgatagc
    26581 ctgggatatg atgcgataca agatgcggga aatgggacgg ttttacaagc ggggcaatat
    26641 gccatcgtat tcgaaaatga atatacactt aatacgggtc gttatacatc gcgtatccca
    26701 tcgtcggcat tagttttaac gatcaacggc tccacctttg gtagcagttc aagcggattg
    26761 gtgggaacct atccgaagca gttatttctg gtacgcagtt taggtgatac gatctcaacg
    26821 taccgctatt ctctcgacaa taccgaagga cattccgata caaaaatcaa tccctcggcg
    26881 gccaacgcgt ccgtgaattg gatgaacagt ctcatcaccg atggcacgcc gggcgctcat
    26941 gcggaagtca acttgtcagt cctcggtcag cgtttgcgat ttgcgccttc cgaaattcca
    27001 tccggtactc aaacagtgat atccgtgact gttaaagatt ccggcatcgc accggtgaat
    27061 attttcaaaa tcgctatgta tcatgatacc aaccgaaacg ccactcctga accggaagaa
    27121 atcattgatt ccctggtgtt ttccgggcaa ttgtattacg gtgacagtgt gatatttacg
    27181 gcaaatacac cgactctctc ctatggcgta cattatttta ttgcacgtat atcgcaaggc
    27241 gaggtctcgc cgtacggtga tacactctac tataacgata tgctcaccga cagtataagg
    27301 acccaaccat ccggggacat cgcactgaac cgcggacgcc tgatattggg tgaaattgcg
    27361 acgcgcggta attcctttcc gatcggtgcg tatgcgtaca atatgggcac gcaacctgtc
    27421 caatcgttta ctgttcgatg gtatgaagat cgcgacggaa attatactac cgcaccggag
    27481 gaacgaatag actccagcac atatgccgga aatcttttac ccggagattc aaccttttta
    27541 cagatcaact atcccaatgc gacctacggt aaacatcgaa ttatggttga agttatttcg
    27601 acctccgtgc aacctattgt cgacgaagag ccgtcaaaca atctgcttgt ggattcgctt
    27661 gaagccggag cacgcgcacg cgatattctg atcagcgaat ttatgtatct tgccgtaacg
    27721 gatagtattc ccgagtggat cgaaatctac aacccttctg cgctgtccat atcactgatg
    27781 aattggcaac tgtacgacaa aacatctcat gtcgtattga cggagcgaag tgacactttg
    27841 cacacaggac aatttggaat catcgtcaaa gactccactt tgtttcacaa aaaatacggt
    27901 gaagcattat cggacgtttt atatatctat ccgacctcca tgccgagcct taataatacc
    27961 ggcggcgata ttataaaact aagtacaccc gcgggactta ccgtggacag tcttaactat
    28021 ttgacttcat ggggcgacga tgaaggtatt agcctcgaac gtaaactaaa cgatagcgca
    28081 tcgtacagca tgagcaactg ggcgctcagc agcgaccctt ccggcggtac gccgggtaca
    28141 aaaaacacag ctacacctca gcttcacgat ctgtccatca cttcacgtga cattctatgg
    28201 acacccgctt atccgacgca aggagaagtc tttcaaatca aagctactgt acgaaatcta
    28261 ggcgttacct caagcggggc atccgctatt ttactctatg cctacagcgg tgcagattct
    28321 acactattac agacaacgaa ttcacctgcc atattacccg gtgattcttt ggtgatcgtc
    28381 tcatccccga ttaccaacgg acctcaaacc tatgtcggca ttcaaattga ttacgcggaa
    28441 gatgaacgtc tatccaataa ctttgctaaa cgtttcgtac ctgcggccac accaagacaa
    28501 acggtgatca tcaatgaaat catgtacaaa cccggaaccg gacatacgga atggatcgaa
    28561 ctttacaacc gctcacaaaa cgcagtggat ctgcgttcat ggcgtatatc cgatggtaat
    28621 gccgacctga ccgcaccggc tactgctaaa actataaccg ttcaaagtgt catacttcaa
    28681 ccggatagtt ttatcatcat cgccaaagat tccgttcaat tttcatcgca gtatggaaca
    28741 cctttgcacg ccgtttatct gagcagtatg ccggcactca atgatccggg cgatatgatc
    28801 accatacaag acagcttagg tagtatcgtt gatagtctgg tgtatctatc cggttggggc
    28861 ggcagttcgg atcgtacatt ggaacgacgt ttctatgatt ccgtttcgta tttaacttcc
    28921 aactggctta gctgtgaaag ccctatcggc gcaactccgg gtcaccccaa cagcgtcaag
    28981 ccattgcatt atgatatatc tttaaatgca tctcccttgc acgtaacgcc ttcgtatccg
    29041 cgtatcggcg aaccatttac aataactgcg ccgatataca atcagggctt ggaacccgtg
    29101 aatgatgcct ctctgcgatg gttacgtatt cgcccacaac atgatacctt gattctatca
    29161 gaacagatca tcggtatgtt gaatgccggg gattcattaa aaacttctta ttcggacacg
    29221 gcacgccaaa tcggtgattc catcgttgcg cttatcctaa gtgtgtcgga cgaacgtact
    29281 ctcaataata aatccgctgt tcctctgata acctccgctc gtcgtaatgc actggtgatc
    29341 aatgaaatca tgtacgatcc cggctcgaac agacccgaat ggatcgaact gtataatccg
    29401 ggcgcggata ctgttgatct taaaaactgg cgtctggcgg acgaatccac catcgcaaat
    29461 cctaaaactt ttgccggtac gtccttacgt attccgtcac aacattacgt catcattacc
    29521 aaagatgcat ccgcacttgc gctttcttat ccgttactat catcgcccgt cgtgaccatg
    29581 acctcttttc caactctcaa tcaaaccggt gatttgatac tgctgacgga tagtctgagc
    29641 gtaacggttg acagtgtatt ttacgaaaac acgtggggcg gtgcgaacgg tttttcgatc
    29701 gagcgtatca gcgcaaccga tgacgccaat tcaccgaata actggaaaac ctctgaagcc
    29761 aatgccggcg ccacaccggg gggaatgaat agtacaacac cggtggctct ggatctcagc
    29821 attcgaacga atgatgtatg gtttgatccg ccgtttcctt caccgggcca acccgtacag
    29881 atccatatgc gtattcgcaa taaaggcctg caaaacatca caaacgcagc ggtagtgcgc
    29941 gtctataacg atatcaataa tgatagtatt cctcagataa cggagatcat tgattctacg
    30001 accgtaccgg tgttattagc cggcgactcg cagtccgtta ccatccaatg gacaatcccc
    30061 gcttcagcat ctcgtcgttt atccaaagcc gtatctatgg gatcccgcaa aatcattatc
    30121 cagcttgatt ttgctagcga tgagcgcact gaaaataacc tcctatttac tgcattgccg
    30181 ttgggtacgc cgtttcagtc tatcgtgatc aatgagttta tgtatgagac ggatacttcg
    30241 cgtacagaat ttatcgaact gtataatccc ggtactaccg catgggactt gaaagattgg
    30301 aaaatcgccg acggtagcag cgtcgtatct atcgccgacg tttcggtttc atttttatca
    30361 ggcgcctata aagttttggc gccggacagt tcattcttcc gtaaattttc tactaccccg
    30421 gcctcgtccg taatcatcgt gccgggttta ccaagcctca ataatacggg tgatgccatc
    30481 cgtctagccg atgacctcgg cgcaacggtg gatagcctga cgtatagtaa cacatggggc
    30541 ggctctaaaa atatcagcgt tgagcgtatc gacgccgacg tctcatcgga tgaaccaaac
    30601 aattggacaa cgtccttaac ggcgcaagga cacacggccg gtagtgtcaa tagcattgta
    30661 tctgctgaac cttttgtcaa acaatcgctc atcatcaatg aaatcatgtt tagccctttg
    30721 agcggcgagc cggagtatat cgaactatac aaccgtagcg aaaccgccat ttccctgctc
    30781 aattgggcca tcacggtcgg tgacgataaa acacttctcg cgcaaaccga tatatccgtt
    30841 cctccgcatg catacgtcgt aatcgccggt accaaaacat ttccagatcg ttacgatttg
    30901 gataagacac agattatcgt tccgttaacc aaccttgccg cactaccggc taatggtacg
    30961 gcgctggtgt tgcgggattg ggtgggcact acgattgaca gcttagctta tacggaactt
    31021 tggggtggcg gagacggtta cgctttggaa cgaatccaac cggacgccgc atccgatctg
    31081 atgacctcat ggcgaacatg cgtattcgtc gaaggcggca cgccgggaaa aatcaactcg
    31141 gtatacaacg gcgataagcc taagaaaatt cgcatcaccg ccgatccgaa tccttttttg
    31201 acagatgtcg cggagaacac cacaatcact ctcgaaatgc cggtaactta tgcgcgggtt
    31261 acgatgaaaa tatatgacag tcagggtcgt cttatgatta cattactcaa caatcaaccg
    31321 accggagctc accgcgaagt gatatgggac ggacgagaca aaaacggcca acgcgttcgt
    31381 atgggaatat atattatttt tatcgaggct atcgaagaat ccggcggatt taataaaacg
    31441 gctaaacaaa cgatcgttgt cggaaaacga ttataaaaaa aataccgctg cctcagaaat
    31501 aaatatatac ggataaattc ctttttctcc tgagacagcg gttcaaataa atttcacatc
    31561 agaacggatg ttccatagat aaataaacaa agacctgctt ggttttagag gaaccgttga
    31621 gttgaagcgg tactgataat ccgggcacca cttgcatcga accgatttct ttggcaaaac
    31681 agaaacccgg attgatggta tgtgttcctt tattcttcag cgacgtcccg tcaacttcat
    31741 cttcaaatac actcacgtat tccacgaaaa cattcaggtt ggattttacc aaccatatcg
    31801 cgctggcgcc gatgttatat gctttttgaa ttttcttttt gctgcctaca tccacaccgg
    31861 ggaccatcgt catacccaca ttcacatgcg ctgcccattg ggcggagaat cgtttactga
    31921 caggtagacc gatctgatag cccattttgc cggagcctaa gcctttatta tcatcaccgg
    31981 tcgggagcag aaccgtaaag cgcggtgaca cactgtatcc cgcaggatcg gccatccact
    32041 gatatcggta gttaacagct acatcgccaa aaccgctcgg accgctgtga agccactgat
    32101 aaggcaatgt atagctcaac tgatgttttt cctgatgcgg taaaaaaaac atcggccact
    32161 cctgcgtcaa accgtattcc caatctttcg acggtcttgt atagataaga cccgaaatgt
    32221 gctgcacgac ccgcgcttcc tgattgtaag cttcttccag cataaacgaa ttgtcactga
    32281 tcgcttcggg atattcggtt tgagcccaga ggctttgaac accgaccaaa atgaagagta
    32341 accgcttaat cataatgatc tccttgttaa tgatgatgtt tacgtttgat gacatgaagt
    32401 ctttgcggac gaatcgtaat ttcggaaatc actgtaccgt cgcgctgcgt aagtacgtgc
    32461 gcaacggtat ccgctacctc ctgaggttca atatacgtat cggtttcgtc agacggtgaa
    32521 aaattcagat tatcgtaaaa tgccgtacgc gtaatatccg gattgatatt cacgactttt
    32581 acgccttgtt tgcggcattc atcaaacaaa gaagccgaaa aatgccgtaa acccgctttg
    32641 gtggcggcat aagcggcgcc catacggctt ggttccaagg ccgttacgga agcaatatga
    32701 atgacaaacc cgcgttgttc gcgaatacgg cgtaaaaaaa gatgcgtaag aataagcggc
    32761 gccgtcagat tggtcgttac catatcctgc agtgtgcgta aaggaatttc ctcatgcggc
    32821 gcaaaatatc cgaatccggc gctgtgaatt aacacatcca gcgattcttc cgtcggccat
    32881 tttttttcaa cttcttcgag cgcttttata tcccgaacgt cacacactat cggtacgaag
    32941 ttcgcgtgac gccagtctgt tttatcaaaa ttgcgtgcaa gtccataaac ccggtacccc
    33001 atttcagtaa gcttctgcgc cgtggccaat cctatcccgg atgaagcgcc ggttatcaat
    33061 gccgttttca taggattatc ctctcctctt ggacataagc actcattgtt ttacgaatgt
    33121 aatccagcat actttcccga tcgagatcag tataactcat agtttgatct ttttcgtcgt
    33181 acggataaaa caaaatatcg gaatcaacac gcatagcacg aatattatta agataatttt
    33241 tattcatacg aaaaacaccc agacttacat catgcagcgc tgcgcccgga atacgttcga
    33301 atatgtattc aatacaacgg ctatatatgg cttgccaatc cttgattttc aaaatcggat
    33361 ctatacacaa acgcactttc cgtccgtctt ttacagcttg tgccatcgcc gaaagtcttg
    33421 tatctaatgg cggcgtgccg gattcatata cttttataat ctcatccggt gagcatgtcc
    33481 atgccaaaat cacgttatct gaaaccggcg tatcgcgtat ggcatcataa gcgccggatt
    33541 tggtgcgaat ttcaaccacc agatcccgtc gttgtgcggc gaaatgaatc cagcgatgac
    33601 actgtgggag aaatgactcc atcgccagca aatcggtatc gtacgataca cacaaataca
    33661 gtggaccctt ggccaactcg gctacgacgc tatcaaaaaa agcatccgga ttgacaaaga
    33721 acaaaagatg cgctgacgga aacattccct gcaaataaca ataatcacaa ttgtaaacgc
    33781 agttgagcac catcgcattg taaaaaaatc gatcatgccc gaaactcggc gtctgcggtg
    33841 aaccggcgta taaataacct tcgcgtttga cggccagaat gatatccatc gcccgttttt
    33901 gaagctgaaa gtcttgatgc ttgcgattga aaacgtcttt ataatgtcgt atcggaatgc
    33961 gtcgggctgt cggaaaccgc gcaagaatag tttgcgcgtc cggataagct tctgcgccgt
    34021 cctcgatata aatacggcta aaatgtttac tgaatgagct tttgtcgaag ccgctccacg
    34081 cgtgcattgc gctcctgttt attttctcca gcccattgtg tcatatccat gagatcaccg
    34141 ggtaagtaac cattacgaat tttataactg cgtgccgcat caaaaagcgc tatacgctgg
    34201 gcatgactgg gattgatcat ttcaaaaata tgctgcatgg tttcctgttc attcacactc
    34261 agtgtttctt tatcaatacg catcgctgaa agaacatcta gacacgagga aaacttcggc
    34321 aaacattgca tagtaagatt ttcgataaaa tccgaagtca tacccttgat ttgtaaatga
    34381 tcggacacga ttttcagaac gtgaatgcgt tctaacggca gaaaacgcgt tgacgcaaca
    34441 aaaaaaccgc tcgcctccat atcatagagc gcatggtcag cggaacctac acactggttt
    34501 tcttgcggcc gatctacggt tcgcaaaaca ctctccccca tcgtcaaacg gatacttccg
    34561 tcgggataat aatctctgcc ggacactacg tccgtgatct tgtgaatgcg ccaaaacgag
    34621 ccgatatcaa gtgttgtatc cgatgcgccg cagattccga aattccacaa cgtatgaaga
    34681 tggcctgtcg tgtagaggta cgtagcggcc gttgcagatt tgatttttcc gatcccgctg
    34741 acgatcagac ttatggtttc gttttcgaac acttcaaacg ccgtatgcgc gatgtttttt
    34801 ttcaatcgcc atgcgcgtat cagcggcgct gcttccgggc gtaacgcgac gacgataccg
    34861 tgcttagtgt tttttttcac aaagctccag taatacgccg cccgtggact taggatgcac
    34921 aaatgctatt ctggcgccat gcgcacctgt ttttgggact tcatcaataa gctgaaatcc
    34981 tttagccttg aggtcggcga gtttttcctc gattcccgtc acaccgatcg cgacatgcgc
    35041 aatgccgcct ttaccgttat ttttttcaat gaatttggta atcgggcttt cgggatcagt
    35101 acccgcaagt aattcgatcg aagattcgcc gactttaaaa aaagccgtct cgactttttg
    35161 atccgccacg atttcgcgac catggtattc aaatcccata gccttatata attcgatgaa
    35221 tttttcaaga tcgggtactg caattccgag atgatcgatt ttttcgagtt tcatctgcgt
    35281 tatccttgtt ttgccgcttt aagcgtattt ttgagtagca taacgatcgt catcggaccc
    35341 acaccgccgg gaacgggtgt gatggctgac gccacttctt tggccgaatc aaagtgaaca
    35401 tcccctacca aacgtgatcc cgatttggtg gacggatcgg atatgcgatt gattcccacg
    35461 tcaatcacca ccgcgcccgg cttgagcatt tcgccgcgta tcatttccgc acgccccacc
    35521 gccgcaacga cgatatcggc ttgcttgata aaatacgtca tgtccggtgt tcccgtatgg
    35581 cataccgtca cggtagaatt agcgccttta gctttttgca ccagcatatt ggccaaaggt
    35641 ttgcccacga tattgctacg tcctacgatc acggtatgtt ttccagacgg atcggcttcg
    35701 gaacgcatga gtaattcctg cacgccggcc ggtgtacatg gcaaaaaaat cgcatcccct
    35761 atcgccaaac gaccgacgtt aaaaggatga aaacaatcta cgtccttggc cggattgatg
    35821 gcgttgatga taagcgtttc attgatgtgt tttggtaaag gcaattgcac taaaatgccg
    35881 gtgaccgacg gatccttgtt aagttgatca atcagggcca gcagatcttt ttcaggcatc
    35941 gtcgcgggtt gtttgatcgt ttcatgtgcc atgccgactt gttcacaggc caaccctttg
    36001 ttgcgaacat aaacttgtga ggccggatct tcgccgacta agacaacgac caatttgggc
    36061 tgcacatttt ttattttaag cgcttcgact tcttgcgcaa tttcacgttt gatggattcg
    36121 gcgatttgtt taccatcgat tatttttgca ttcacggatg tatcctcgta tatagattcg
    36181 ataatggagt tatttttaca aaccgattgt ttctaatatc cggccgcata tcccataatt
    36241 cgttgatccg cttccccgtg ataatatccg gtccgtggat caatccagat ggaatgggca
    36301 tcaccgataa atggcttctc tgataatata tagccgtttc gtaataaaaa agcgcgccgc
    36361 gtttcgtcca gtttatcttt ttcgtaggat aacacattcg gtgaccacgt atgactcaac
    36421 cgcgggcgat ccaccgcctg ccgcaaggaa tccccataat ctatcatgcc gatgatgatt
    36481 tgcagtacgg tattgatgat tgttttaccg ccgggcgaac ccgtgatcaa aaacggttta
    36541 tcctctttga gcatgatcac cggagtcatc gaactcaaca tgcgcttatg cggttcgata
    36601 cgattgggtt tttgcccatg accgggaaag cgattactgt ccggaaaaac atcaaaatca
    36661 tgcatctcat tattgagcaa aaatcctaaa ccggtcacca cagctttgga accaaaatta
    36721 tcttcgagag tataggtatt ggtaacgcaa ttaccgtttt tatcaatgac cgaaaaatga
    36781 gtagtctcgg tcggttcctg tttcatggct aaggccggca ttttagcatt gatatgcgcc
    36841 gaatcaatcc atgaagccaa cttgcgagcc agcgctttat ccgtcagagc ggaaaccgga
    36901 atatctgaaa aatcaggatc accgagataa actaaccggt ctaaaaagga acgacgcatc
    36961 gtttcgataa cgcgccgcgt tgatatcgta tcgtcatgca gtgtaaaatt ttcgagtatg
    37021 ttcaacatct cgatcaatgt cactccgccc gaagacggcg gcggcatgct gtatatggta
    37081 taaccgcgat aattccctcg tataggttcc cgcaccaccg cttcgtaggc cgctagatcc
    37141 gcatgcgtga tcagtccgcc atgcgctttc atgtcatcgg ctatttgtgt ggcgatttta
    37201 ccccgataaa aaacatccgc gccttcatcg gctatagcac gcaacgtcgc cgcaagttct
    37261 ttttggatca aaagttcgcc ttcggcataa ggcgatccgt cgggtttaaa aaacatgtgc
    37321 gccgtttcat caaaccgttt gagcgccggc gcaaattcac ttagtcgccg tgcttctgcc
    37381 gcttcgagga taaacccttc ttccgccaaa cgtatcgccg ggtttatcag ttccgaccag
    37441 ggcaacttac cgtacaattt ccatgctttt tcaagtccgc gaactgttcc cggcacaccg
    37501 gcaactttgt atccataatt actggtgatc gtatcctcgc tgccatccgc attcaaaaac
    37561 atacgtgcat gcgcttgata cggcgctttt tcacggtagt ctatggcttt cacatttccg
    37621 tcggatgttc gcaccatcat aaatccgcca ccgccgatat tgccggcttg cggatacgtc
    37681 acggccaaag caaaagccgt agcaatagct gcgtccacgg catgaccgcc tttttgtaaa
    37741 atttccaagc cgacacgcgc cgcattctga tcaacacaga ccacgacacc gttttgtgcg
    37801 gttacggaag atgatatacg cgatgtttgg tgcgaattct ggcaggaggg aaaaattatc
    37861 agcgcggtaa gaaaaaaaat ccgaagaaac ataatcccaa tctcaaacta aattgctgaa
    37921 gcaatttagc gaatatgata aaaaaccaca agcaacgatt gtataacgtg taagctccgc
    37981 ttcgcggctt taagtagcta aaacgaagct tacgcacggt tcttaaaaaa tcacatcagg
    38041 gcttcacgca ccgccgcttc aaatttggcc ttggattgca agccgatcat ggtttttacg
    38101 gatttaccgt ttttatcaat gatcaccgtc gtgggaaagg cctgcacttg aaactgctgg
    38161 gcgaacgcat cgtcaccata ccataccgga taattgatat taaattcttt cactgtcggt
    38221 ttcgaagctt cggcggattc ggactcgaga agaacaccga gtacgactac atctttgtcc
    38281 ttcatttcgt tatgcaaagc gatgagatcc ggaatttctt ttttacatgg tccgcaccac
    38341 gtcgcccaaa tatttaataa aaccgttttg ccgcggtatt gtgaaagcga aacggtattc
    38401 ccgtcaagat ctttgatcgt aaaatccacg gcgccacccg atccgggttt attgcaaccc
    38461 gatgacatga gtcctgccaa aacaaatatc gcaagagacc aacgcataaa aactccttat
    38521 gaagttattt ttgacttttt tgtagtccgt cagctaaaaa tgatttaaaa atttcagggt
    38581 cgcgttcaag cccgccgaaa cgagccaata ctttttcgtc aggcgtgatg ataacgtaaa
    38641 acggcaaagc tacactgccg actaaacgct gctgcaagtc ctgataacgt tccatgtttt
    38701 ctccaaaatc agtccacaac cgcgtcaaca ccatacgatc aaaatagaat tgaacatcag
    38761 cgcgcgtaaa catggttttt tccatgaggc gacaattggt gcaggtcttg ccggtaaaat
    38821 caataaacaa gggtttgccg gaagtacggg cttcacgaag ggcggcttcg tagtcttcga
    38881 tccatgtttc gtgtgactct gtactcttgg catttccggc cgtgcgcact acgccataat
    38941 ccgacggagg caaatacgca tccatatcag cttgtaacgg atttccaaaa agcccgctaa
    39001 gcaggtaaac cgtaaacgaa ataaataaaa gtccggtcat tgtacgacca aaacctattt
    39061 cttcgacttc atcttcgaca gaccagcgaa ttttccccaa cagataaata ccactgacgc
    39121 caaacaatgt gatccatatc gaaagcatgg ccggtcgtgt gagccattgc agatcccaga
    39181 ctaagtcaac attactgaaa aatttgaacg ccgcggctat ttcgaccaat cccatgacga
    39241 tttttgtcgc atgaagccat ccgccgcttt tagggagatt agcgaggtat tgcggaaata
    39301 aagaaagaaa gaaaaacggt gttgaaaacg ccgtcgcaaa acacaacatc ccaatcaccg
    39361 gcagaaacca ctctccgtcg gctgccgcca ccatcaaaag cccgagaaac tgcacggtac
    39421 aggtaaatga aaccaaagta aatgtaacac cggccatcag cgtaccgatc gtttgtccgg
    39481 tattggcttg ctgttgcgaa aaattgacaa taaacgacgg tagccgaatt tcaaaaaccc
    39541 caaataaact cattgcaaaa acgatgaata aaattccgat cgcgagattc acccaggcgt
    39601 tggctgcaaa attttgggcc ccggtagcgc cgacgacaag cgacataagc catccgaata
    39661 acgtaaacga tgcgataatt ccgacgacat agatcaccga ctgacgcact gcccccgccc
    39721 ggcttgacgc cgaatgtttg gtaaaatatg aaaccgtaat cggaatcatc ggaaagacgc
    39781 agggtgtgag tagcgccaaa agccctgtaa gaaatgctaa tccgatgaat gagcccaagg
    39841 attctttacg caaactttcg tacgaggccg tacgctcctg tatcgtcgaa actttatccg
    39901 gcactgtttg agcaacgcta tcatgggacg cagagctttt gttttcgggc tcagcgatcg
    39961 aatggctctg taggctgatg cttccaacgg cttgattagg atttatttta tttatgggtt
    40021 ttatcgttcc cgcggataat ccgatccatt gttgcgaaat ggtatgcgta gcccgtattg
    40081 ttttaggcgg aaggcaatac gattcggtac acgcctgata cgtcatcgcg acggttatta
    40141 tgtcaccggt tttagccgtt gcgctgagtt tgagcttctg cttaaatacg gttttgccgg
    40201 agtgccacgt cacgtcaatg ccaaatcctt catcatgatg acgttttccc tccgggctca
    40261 tcacttctcc gacgagctct gtaccttccg gcaacgtaaa tttaaattcc gttgcgatgg
    40321 gaccgtcgag tttttccatt ccataaacgt ggtatttcga atcaatatca gctacgattt
    40381 ccaaaaccgg ttgaccgttt tcgatcgcct cacgggttga aagtttaact acgttggttt
    40441 gcgcacgtgc cggtatgacg gagaaaatta agtatgtaag aatagcgaca atcgttcgct
    40501 ttgttttata catcatgttt ttccttaatt gcaagttgcc cgcaagccgc cgcaatatcc
    40561 ctgccccggg agcgacgtaa tgtcaaaggc gaggaaatac ctgaaactaa tttcataaat
    40621 cgcgcgatat gctccggcga agggcgtttc agatcgggtc cttcgatggg attgtacggt
    40681 ataacattca ctttacattt tcctaattgt ttagccatcg tacgtaagcg tttggcatcg
    40741 tcatccgaat cattaacgcc cgcaagtaaa acatattcca gcgtcgccgg gttttttcgc
    40801 cgggcattga gatattggat ggattccagc aaatccttga gcggatattt tttggtaatg
    40861 ggcatcactt tttgccgtac ctcatccgtc gtggcattga cactgatcgc gagattgtaa
    40921 ggcaaatcgg cgtcggcaaa ctcataaatt ttggggataa tacctgaggt ggatatggtg
    40981 attcgtttag gcgccaaacc gggaccatgt tccgatatta taatgcccgc cgcattcatg
    41041 acattttcat aattaagaaa cggttcgccc atgcccataa aaacaatatt ggatatctca
    41101 ggatcgattt cacgacggat atacataagc tgcccgatga tctcgccgac actaaggttg
    41161 cgacgaaacc ccatccagcc tgtggcgcaa aattggcatg ctaccgcaca gccgacctgg
    41221 gaggaaatac aaaccgtttt acgcccctca tcgggtatat acacggcctc aactttttca
    41281 ccgtccgcaa gctgaaataa aatttttcgc gttacgtcgt tctctttttc gccggaagtt
    41341 tggattttga tcacggtatc ggttatgata acggcggatg catgcagctt atcccgtaag
    41401 gatttggaca aatccgacat atcatcaaaa tttgtgacac cgcgtttata catccattga
    41461 aaaacctgta cggcacggaa ttttttttcg cccaaagctt caaaaaacga ttccagttcg
    41521 gacaatgtct tgccgaaaat attgatcgtc tcgggcttta aattttgatt ttttgcaatg
    41581 tttttcatcg ggttgcaaca tattaactcc ggtgaagata aacaagattt tttagccgcc
    41641 ccttgcacgt cacggcaaat cattgtatca tgcgccatga atttttacgc acgtgtaacc
    41701 gtatttcttt tatccttggt ctccatttta ttaacggcat gttccggaaa atcatattca
    41761 accgataccc cggaaaactg ccataatgcc gtggtgacag cgatccgaac gcatgatttt
    41821 ggcgccttat atactctgat agacaccgcc attcgttccg atgtacgtac atactttcac
    41881 aatacacgct ctgtattatc catgatcgat tcgtcctatc ctgccagtca gcatatcgca
    41941 gctcgcggtg atctgacatt acgattcagc aatgactctc tgacgatggc gcattttgaa
    42001 aaaatcagta acgacacgat tttgttttcc gatgtttcat cgcgcagtat cggtaatgtt
    42061 tcgtggtggg aaaacattat ccaggcctat cgtgcacacc ctgaaaaaat catcgaggaa
    42121 gatggttttg cttctgtcat cacgtccgcc ggagatacgg tactctatca acgttataac
    42181 gacggtaaat ggtactgcct attgccggtt catacatttt ggaaaactct tgccacggtc
    42241 agtcggcaaa acttagatgt cacccgtcgc aatagtatcg ttttttcatc cgcattacgg
    42301 gatacgctat gagtttaaag ttaccttggg atatcgaatc gctgcatctg atttgcgaac
    42361 gtgacgaaga atcttggctt cacggtggca cgcattattt ccatgaaaat aattcttcga
    42421 tcctcgccgt agcccatctg gattgtgttc aggcatatgc gggatttgat tttgaagaaa
    42481 ccgacggtca ttcgacggtg cattgcacta cattggacga tcgccttggg gtatatatca
    42541 ttacggaatt acttccttcc atcggagtga ctgccgatgt actcttaagt accgatgcac
    42601 ggcatggcga aagtacggca caaatgttta taccggaaaa aaaataccat tggatttttt
    42661 cgtttgatcg aaaaggatcg ggcgttgttt tatatcaata cggcgatgcg gaaatcctac
    42721 agctaatgca aaaatacgga tttgaagtgc atgaaggcta ccgctcagac attgttttaa
    42781 tggatcatct ggagtgcaaa gcgtttaatt ttggttgcgg atatcatgaa cctttgacag
    42841 acaaatcttt tgcggtattg gacgaatcgg aggtaaatat tctccgattt caacaatttt
    42901 acaacgatca taaaaacacg ctgatgccgc atgtgccgtc gtatttataa cattatgcaa
    42961 ttttaattta tcaaccaatc atacttttta tgtccgaacg tttattctta ttagatggta
    43021 tggccttggc ctatcgcgca tattttgcat ttattcataa tcctttgaaa aatgctaaag
    43081 gtgaaaatac cagcgcggta ttcggtttta tcaatgcttt gatcaaaatc cgagatgaag
    43141 aaaaaccgga ttatatcgcc gttgcgtttg atcttgctga acctacgttt cgacacaaac
    43201 aatatagcga atacaaagca acgcgtgaaa aaatgccgga agacatggtg ccgcagcttc
    43261 attatctcaa agaactgacc gaagcgatgg gcgtacctat tctcgagtac ccgggctacg
    43321 aagcggatga tgtgattgga acattgtccg tgcgtgccgc acagcaaggt atcgtagcgc
    43381 acatggtgac gggtgataaa gatttcatgc agctgatcgg ccccaatatc gtaatgtata
    43441 atcccttaaa aaaaggcggc gaaaaagaaa tcatcggacg cgatggcgtt cttgaaaaat
    43501 ttggcgtcga accgtcgcaa gtcaccgata tactgggcct catgggtgat gccgctgata
    43561 atatacccgg cgtaaaaggc atcggcgaaa aaaccgcggc caaattgatc aatcaatacg
    43621 gctccatcga aaaaatatac gaacaaattg atagcatcaa aggaaagttg cgcgaaaacc
    43681 tggaacaaca taaagaggat gctttccttt ccaagtcgct cgcaacgatt gcactggatg
    43741 tgccgcttcg cgaagatatc catgatctgc gtggtcggac gccggatcat caaaaagtca
    43801 ctcgccttct taccgaactc gaattcaagt ctctgattaa aaaaatttta aaaaacgaat
    43861 ctgatttatt cgtcgaggat acaccgcttc agcacgaacg tacgccggaa cccgctcgta
    43921 attatcatac gctccaaacc gaagtggcta taaaagaact tgtcaccaag ttaacgaact
    43981 caggcggctt cgcgtgggac acagaaacga cggatgccga tccgatgcgt gctgagttag
    44041 tgggcttttc attcagttgg accgagggtg aagcctttta cataccttgc aatggggacg
    44101 ttcctgtcga cacaatttgc actttattga aacccgtatt ggaagattcg actattccca
    44161 agtgggggca aaataccaaa tatgatatca tcatcgtatc acattatggt atcgaagtac
    44221 aaggaactgc ttttgacact atgatagcgg ggcatctttt gcactccgac aaagaggtca
    44281 acattgacgc tctagctgcg tcctatcttg ggattgaaaa aatccccaca acggatctga
    44341 tcggcaccgg gaaaaatcag attacgatgc gggatgtccc catcgaaaaa gtcagcgctt
    44401 atgccaatga agacgcggac tgcgtcgtac gtttaaaaaa tgtcatgtcc cccttgctca
    44461 aagaaacaca tagtgcaaaa atttttaata cgattgaaat gccgcttatt ccggtgcttc
    44521 agagaatgga acgccacggc gtatggcttg atttggaaat tttaagtacg atgtccaaag
    44581 attttgccat acaaatcata caggaagaac aacgcatttt caaagaagcc ggaacggact
    44641 tcaatatgaa ttcgcctcaa cagcttggtg aaatactttt tgaaaaactg caaattcata
    44701 aattggccgg catcgaaaaa ccgaaactaa cgggtaagac caaacaatat gccacggatg
    44761 tgaaaatatt agaacaatat cgtacgctac cgatcgttga ggctattctc aattaccgcc
    44821 agctgaccaa acttaagtca acttatattg acggccttcc cccgcttcgt aatcctcgaa
    44881 ccgggcgtat tcacacgaca ttcaatcaaa cgatcgccgc tactgggcgt ttatcatcaa
    44941 ctaatcccaa ttttcaaaat attccaatcc gtactgacat gggccgtgaa attcggcgcg
    45001 cctttacagc acaggaaaaa ggttgggttt tactatcggc ggattattcg cagatcgaac
    45061 ttcgtgttat ggcgcacatg tccggagatg aaaatttaat agcggcattt tccgaggatg
    45121 ccgatattca cacgagtacg gccgcaaggg tgttcggcgt tgaggccaat gaagtgacaa
    45181 aggacttgcg ccgaaaggct aaagacatta atttcggcgt catttacggt atttcaccgt
    45241 ttggattggc tcaacgaatc ggtatgagtc aagccgaagc gcgcgagttt attcatagtt
    45301 actttgctac atatcctaaa gtaaagtcgt atatcgaatc ggttattgcc aaaggaaaag
    45361 aagacgcctt tgtaacaact ctctttggtc gccgacggta tttaccggac ttacaaagta
    45421 aaaactttac ggtacgccaa tttgcagagc gcgcagcgac caatacgccg atacaaggaa
    45481 cggccgctga gatcataaaa atggccatgc tcaaaacaca tgcggcgctt gaaaaaagtt
    45541 cactttcggc aaaaatggtt ttacaggttc atgacgaatt ggtatttgaa acaccacagg
    45601 ccgaattaca gcaacttgaa gcattagttc gttcgtccat ggagtctgct gttcagatga
    45661 aagttgcact taaggtggat atgggcagcg gcgacaattg gctagaaaca aaataaaatt
    45721 gatataacaa ggagacaata tggcttccgt gaaagcaagt aagatcaatt acaaaacaat
    45781 aatcattgcg gtatgcggat taatactcgt gggattggca atttggtact tttttttacg
    45841 ttctaaagaa tatccgggcg tcccccgtac ggccgtaaag tattataaag aaggtatgga
    45901 ctcgttagat gccggtcgta tcgaagccgc cgcaaatctc ttcactaagg ccgtggattt
    45961 ggatcatacc tttgcggatg catgtgccaa gttggcagaa acatattatc gcgcttccct
    46021 tcaacataaa acaaacaaaa acacaaaaat gcatactgct atgcttgatc aatccaaacg
    46081 ctttatgctt atggcgtttg atgctcaagc ttctaatggc aatgcacact ttgtaagggg
    46141 tttattggct tttgataata aagcatatga tgaagcgtta cgagattttg aacatgcaga
    46201 aatgtatggt tacaaaagct tcgaattgca tagcatgatg gcttatgcat acaatgaaaa
    46261 ggaaaaaacg gctctttgtg tagaacagta tcaaaaagcc atcgctataa agccggagga
    46321 tatgaacaca ttatataatc taggcgaatt atacttccaa cttggaaact ataatatggc
    46381 atccgattat tttagcaaaa tggttaaaat agatccgaca cattcacaag cacgtattaa
    46441 ctatgctttg tcagtttgga aaaacggaaa tccgaactta ggaaaatcta tgatcaacca
    46501 actactggat accgacggaa aaacaccggt gaatttatat aatgccattg cctggcagct
    46561 tatcgataaa gatattgaca tagcaatggg actcaaatta gctcaggccg ctaacggaat
    46621 gaaacctaat aatgttgaat caattgatat tttaggttgg ggctactata aaaacggtga
    46681 atataccaaa gcggtagaat acctatccca atcaatgaaa aaaatgccat cggatgaagt
    46741 gaaaaacaga ttaaaaatgg ctaaagataa acttgaagag tcaaaaaaat aaatattaaa
    46801 aacaaactag aacccataat ttaaaattct acgattgacc gcctaacgcc tgctctacca
    46861 accgcaggcg ttttcttatt tcattttgaa atatcgattt tggaaatttt actaacaatt
    46921 gtcggtagag ctgaatcgcc ttatcgggac gatttaattt ataatcgtaa atttcggccg
    46981 ctttttttaa agccaaatca gcctgaatgc tttggggttc agctgtcaaa acttgataaa
    47041 acgtactcac agcttcattg tactgaccga gccgttcgtg ggttattcca atacgcatca
    47101 aaacccactc ttttaggcga ctctcctcta gtgtttcact gatatggttg agttcacgca
    47161 aagcttctgt atactttcgt tgctctgtca gcaaatcaga ttttgcgaga gccttctgaa
    47221 acgataaatt tttcttactc tcttgcaaaa gtaaaatgaa agaaagcgcg tcattgacat
    47281 ataaaccgtt aggtacttta gccaaagatt gcatccgctt gtagcatgta tctatgtgac
    47341 cgttataaaa atcaattttg gctaaggcaa attcagcacg ctccaagtaa atagccgtgc
    47401 ggttgatccg catggcttct ttataacgct gagcactatt ttctatttcg ccacgaatca
    47461 aatggcattc gcccaagcga aacaacgatt cgatagcaaa attattttta gcaaaatttc
    47521 ggtacaccgc ttcataatga cgaatagctt catcaacgtc aaacattcgg tttaaataaa
    47581 tttctccaag ccgataatgc gcctcagccg cccactctaa atttttatat ttttctgcaa
    47641 tgtttttgta aacttcgatg gcttgatttg tataacgtaa ttcggctacc atagcttggc
    47701 caagcgatgt atcctgaaat gcctttacta ccgatgcgcg tgcatcttga ttttcccaac
    47761 atcgggccaa acccatttca gcttgcggtg cattcgggta ttgataaaga aatgatttgt
    47821 aggcttcctc agctaccgta tatcgttgtg cgccaaatgc catatctgca aatgcgagaa
    47881 tctccgtccc ttgtttttga aattgctcgt ctaactgaat tgtggtagca aacgccgcat
    47941 ccatgcgtcc ggatttaaat tgaagctctc caataagctt gagcagttta ggttctttgg
    48001 gtaattgctg aatatagatt tttatggcgt gtacgatagt atccaaatca gcggatacat
    48061 cgctgaattg taatatttgc tgacgcacgt aatcaaatgt ccccatgccg taagaccaaa
    48121 atgataggaa ttcgagcgta gcggcatgat aatcaccgcg atattgctgc aagttggcca
    48181 tttcagcaat aaacagtttt tcgttacgca atgttttacg tgccgattta tacactcgct
    48241 ccaactcatc ccatcggcgg ttttccatta aaatcgaagc aaatacgcgg taatttccga
    48301 tttggctttg ctcagtagaa atatactttt gtattattgt agcagcccgc tccgggcgtt
    48361 taagtttgta ataattatca gctaattcag cccaaaatgc aatttgggac gaatcatttt
    48421 gaatctgtgc caacagtacg cgctctctca attcatattt tcctaaagtt ttatacgcgc
    48481 gtttcaggcg atcagctaca acaatatttg aggggtcttt ttgatataat ttttcataaa
    48541 tagtcaccgc tttttcaaat tgaccatccg cttccagact ttgtgcgagg ttcagcgggt
    48601 caccgctttg cgcgctgatt ggaaccgtct taaaaagaaa aacagcaacg taaaatgttg
    48661 ctgttaataa aacacgactg aacatatcat ccctgattat ttaatggatt ccacgcgaag
    48721 ttttcctagc ccggtttcca agtgtaattc caaaacagca tccgcagcgt caaatccttt
    48781 ggaaatatat aacccgtcac gcttcttcac aaaatcattt tgatcaattc gaattgatga
    48841 taaatacgat tcgtccgtat gtatttttac ggcaaggttc tgaggcaatc gtataataag
    48901 actaccgatg cctacagtaa tatgcgccga agcactttgt gtcatcgtac ccgaaaaatc
    48961 caacatataa tttcctacac cgccttcgaa ctctagtttt tcgaagttgg cattgcctaa
    49021 gccatacata ttaagcttag aaataccggc ttcaattcta aatttattga tgcgatcacg
    49081 attgagtgac gagaagcgta tattggacga actggcgccc atattcagcg taagatcgcg
    49141 aacgcgtagc tgagtaaaat ccatctcact ctcacaggca cccaaatcta ttttaaacga
    49201 tacaggtatc ttgtccgtca gatacatctg tatatcttgg ttgcccacat cgaagaagcc
    49261 cacattgttt tcttcgtcca tacgcataga aatcataccg gtcgtaccgt ttttttcata
    49321 gtccacggtc ggtttgactt cacgatcttc agattgtatc gttattcgag caagcttgtc
    49381 cgttgaaatc tttttaagag ataacttgcc ggcggcatac tcaagacgaa cttttaattc
    49441 cttttcatcc gtacgttcga tctcctgcac atcatattgg ccaactgcag gcaatacatg
    49501 taaaataaac aaccataaaa agtagagtct cttattttct ggattgaacg tcattacaat
    49561 accttatcta tttttccttt aaggaattta ttcaacattt cacgtgcgcg aaaaatccgc
    49621 gcttttactg tgccgatcgg tatgttgagc atttcagaaa tttcttcata cgataattct
    49681 tccgaatgcc ttaaaaatat aacccgtttg tatttttccg gtaattcttc gatcgcgccc
    49741 ttgatcattt gcgttctctc gttacgcatc atttcttttt cagggtgata cgtaagatca
    49801 ggtatctcaa tctgatactc agaatcctta tattgtaccg gtgcatcaat cgaatacgtc
    49861 tgcaactttt tcttgcgaag gtggtctata cagttattgg ttgcaatttt gtataaccaa
    49921 gtactgaatg cgtattcata attaaaatta cttaatgatg taaaagcctt cataaatgct
    49981 tcctgtacca agtcctcggc ttcttctgat ttatgcacca tacgatacat caagccgaat
    50041 atctgaggac gatacctatt catcagtagt tcgaatgctt tttgatcacc accgacagcg
    50101 cgagcgataa actcaaaatc ctctttcttc tgcttttcac gcttttccaa aagaagccga
    50161 ttgggatcct ccggcgttat cggcacatca atatccaaat catcgtcaag tttcggcttg
    50221 gacggtgctt gttcagaaac cactgattgc agcggggttt tcaaagagcg ttgaaccggc
    50281 ttacgcttgg ctttcgcagt cggtttattt ttctttattg cgcgcttggc catttccaaa
    50341 ccaatagtga tacgttaaat gtttattcta agtttcgatt attttcgatt tcttttaaac
    50401 cgttcttatc cagaatgccg taaattaagg gttttttatc cggagcatta tactctattc
    50461 tataagcact ctgtattcga tgcgcgaccg cttttccatc cacgcggggt gaatgataaa
    50521 tttcggcaat gagatcattg gcttggacgg catcacctac ttttttcttt ataactatac
    50581 cggctgtcgg atcaatcgta tcttcttttt tactgcgtcc ggcaccgatc gcaaccgacg
    50641 ctagaccgat ctgcaatgta tccgtagtcg tgacaaaccc gtccgtttgg gcatacaccg
    50701 aagttttgca ttcggcttca ggatacgttt caggccggtc aatataggat aaattaccgc
    50761 catgtacacg cacgatttca cgaaatttat caaatgcttt gccgttattc aaattatctt
    50821 tacaccgcgc ataggcttcg tcatacgtca taccgggatt agcaaacatg agcatataag
    50881 ccgtttgcgc caatgtcagt tgaataatat cctgcgggcc gccgccgcgt aaaacatcca
    50941 gcgattcacg cacttccagc caattgccaa ttccctcgcc gagcggttca tccatattgg
    51001 taagtaacgc cacaaccgat ttgccggccg attcgccgat agataccagg tgttgtgcca
    51061 gattgacggc gtcatcaaaa tgccgcataa acgcgccatt gcccactttg acatctaata
    51121 caagcgcgtc tatgccttct gctaatttct tgctcataat actcgccgaa atcagcggta
    51181 tggattctac ggtcgccgtc acgtcacgca gtgcgtatag ttttttatcg gcgggagcaa
    51241 tttccgccgt ttgaccgatc atacaaaccc cgatggatcg cagtacgtcg ataaattttt
    51301 ccatcggaaa aaaaatatcg aatccggtta tggattcaag tttatccaat gtcccccccg
    51361 tgtgaccaag accgcggccg ctgatcatcg gcacgacaac acccagcgaa gctgccagtg
    51421 gagcaagtat caaagaaatt ttatcgccaa cgccgccggt actgtgtttg tctactttga
    51481 tacccggtat ggacgataaa tcaacaacca ctccggaatc ccgcatagcc aaagtcagat
    51541 ccgtcgtttc ccggaatgtc atcccgttaa aaaaaacgct catcaaccat gcggacatct
    51601 gataatcggg aatttctccg tcggtatacg accgtataag ccaccgtatt tcatcgccgg
    51661 tcaattcctg gcgatttctt tttttcatta tgatttcata tggcgacata gtttttcttt
    51721 cttaaaacaa aatgaacgcg ctccgaagcc tcacttcctt ccgaaaaacc aagattggct
    51781 aattgcgcaa cgcaggtaaa tggtgatgca cggattatcg catggatttc atccaaagaa
    51841 taaattcgtt gttgatgggt ttcatgaaac aaagttccgt ctatttctgc ttcgatttcg
    51901 ttaaattgtg ttcgggaaac gcgatcatag taactgcggc gtttgtaatg atgcgtccga
    51961 tccagttcat acctctgatc gaagtgatca agtgaatttt tttcagtaca gacatcaaaa
    52021 acaaaaacac cacggttcgt gatacactcg gaaacatgat taaagaaatt cagaatatct
    52081 tcagctttca tcagataatt aacactgtca tataggcaaa gcgctgcatc gcattcgaca
    52141 gtaggcttga aagaaagcat atcgcccgta aaaaagtgta tcttgcctga ttgtgcaacg
    52201 gattttttac gtgcggtcat gatcatggtt tcagaccgat cgctgcatat catcgtataa
    52261 ggaaacgcgg ccatcgctgc ggcaaaatta ccggttccgc aggcaaattc acaaacggtt
    52321 gatataccag acccgtaacg ctcgaatatt tgatgaataa atgacgacca tgttcggtaa
    52381 tccacatgct ccatcatgcc gtcataaaa
//
LOCUS       DAOQGF010000007        50594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_257_length_50594_cov_7.572963, whole genome shotgun sequence.
ACCESSION   DAOQGF010000007 DAOQGF010000000
VERSION     DAOQGF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 50594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_50594_cov_7.572963"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PK937_02345"
     CDS             complement(<1..1057)
                     /locus_tag="PK937_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNH31444.1"
                     /translation="MISVDEALHIILSKAKPLAIRTVPFKDACGYALAENIVSREMIP
                     PWDNSGVDGYAVIASDTSKTKQPCMLKVLDTVHAGDVSTKKLTAGTAIQIMTGAPVPR
                     GTTAIVMVEHTRRVGQWVHIQKPAREGDHIRKAGSDIQKNQIVFSRDHIIRPYDLGVL
                     ASIGKSKIRIQTKPTIALLATGDELLSVEEKLKPGKIRASTHFALTALLQKAGYPVIN
                     LGIAHDTPKAIAAQIQKALKADVIITTGGVSMGEKDYVRQAVEKAGIKIHFWKVRQKP
                     GKPMVFGSKGKKLFFGLPGNPVSSLVCFELYVTPAIARMASQPSPILRIPVRLDNPVS
                     KKPGLRHFLRARLYIHDG"
     gene            complement(1044..1466)
                     /locus_tag="PK937_02350"
     CDS             complement(1044..1466)
                     /locus_tag="PK937_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31445.1"
                     /translation="MIRFFWIMVIAVFPLIQTCTQPNEDSPSRRVRQFTRSYFHRKWD
                     AALVHCTDAARSKILNESSFERQLPDIWKEPEISISEFYVKDSIPDPTGTMIYDLVLL
                     DRDATGGFQESLFRITLKSDSGGKISNYQFIRSDYDIR"
     gene            complement(1463..2389)
                     /gene="era"
                     /locus_tag="PK937_02355"
     CDS             complement(1463..2389)
                     /gene="era"
                     /locus_tag="PK937_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNH31446.1"
                     /translation="MNETNNLGEPTSAMIRCGYVAIIGRPNVGKSTLMNQLLHVKLSI
                     VTPKPQTTRQRVLGILNEPGTQAIFLDTPGLIAPQYALQTAMMKIADQSMEAADVLLI
                     MIDGTSKAESDFVQELFKTKLLHSDIPKVLAINKVDRMAKPELLPMMDTYIKTGFFKE
                     VIPISALENDGVEQMKQALFKYLPLNPPFYPPDALTEQPERFFVAEIIREKIFLHYTQ
                     EVPYSTEVIIEEFKERERGKDLIRAIIIVERDSQKAILIGKKGEALKRIGETARKDIE
                     AFLDRQVFLELWVKVKEEWRSNDAMLRSLGYR"
     gene            complement(2394..3734)
                     /locus_tag="PK937_02360"
     CDS             complement(2394..3734)
                     /locus_tag="PK937_02360"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNH31447.1"
                     /translation="MKRILSLIVLTFLGSCALRGQANMGKTGGGPFFFLDAANVAAKD
                     TTKGRVEIFIKIAYSELLFARYNQVLFRSQYEVNYTLADKKGNVVLREIQDKEIVTEH
                     YEETLSDLRFHFSRLTLNIDPGEYILTVVITDKETQKFGQRKLSLPVRTFREKSIGIS
                     DLLFADKIQKDSVDDIIHILPNVFKSFDNEFKKYAVYFEIYNSKYAWKNRDSIGNLPE
                     EKEPVRIRYRVYDKNQKIVTEDSTVREVNQFQTFSSIEIDKSKVSFGKYVLDVMVYGK
                     NGVRATSKAIFDVRLSTFTAPSLSATAFDLDVAIKQMRHVGRNLNTDKILKGTQKEKS
                     DFFENFWRAKDPTPGTERNEIMEEYFRRVDYANRYFTSGYREGWDTDRGMVFIILEAP
                     DDIERHPFDNNDKPYEIWYYYQANLRLLFVDVNQIGDYELMNRQEFESYLYLRR"
     gene            complement(3813..5198)
                     /gene="rimO"
                     /locus_tag="PK937_02365"
     CDS             complement(3813..5198)
                     /gene="rimO"
                     /locus_tag="PK937_02365"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNH31448.1"
                     /translation="MSTTSKRITLPVLGEASRQRAPRSNKQKKVNIVSLGCPKNQVDS
                     EVILGNLKHTRIVDKAEEADTIVINTCGFIESAKQESIQTILEAIELKKRAAKKGITK
                     EIIVAGCLSERYPKELAAEMPEVDAFFGVHQFDKITERIEGHYQQVLFTERILLNQRH
                     YAYLKISEGCNQRCGFCYIPMIRGNLDSKSIADNVAEAEKLASLGVKELICVSQDTSS
                     YGYDFDRSAPNLRQNKHLIELLHALGEVRGIEWIRVMYLYPSLFSDTLIETIATHPKI
                     CKYIDMPVQHISDRILKTMRRNTGKKQTTDLLYKIKARMPDVALRTSMIVGYPGETEA
                     DFEELCDFVREFKFDRLGVFIYSQEEGTYAYDLEPQVEEAVKKERFNRLMEIQREISH
                     AHNMEKIDQTFRVMIDTQSSEHYIGRTMSDAPEIDNEVIITSSRKLKPGQFVDVKITD
                     ATEYDLYAEAL"
     gene            5518..6162
                     /locus_tag="PK937_02370"
     CDS             5518..6162
                     /locus_tag="PK937_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH31449.1"
                     /translation="MARPKESERQDIKALVLRHSKALFLKQGFANVTMRDIAREIGYS
                     PAAIYLYFDNKDQIYYELFNEGFQILYRRQLETAQQSFNDPTDELYSMGQAYIRFAMD
                     FPEYYDLMFVMREPRKFMEHCLNENTGEPIRTDFSEMSYNLLRATIKKCIDQGYFKGF
                     DVEIASFTTWAAVHGMATLILRGMLVAPEEMKPIIFNGAHECLQALIKTSKQTK"
     gene            6270..8636
                     /locus_tag="PK937_02375"
     CDS             6270..8636
                     /locus_tag="PK937_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH31450.1"
                     /translation="MKRVILILSVLCIAHLGAQEKGTIKGKVLDAVGKQPLAGVSVRI
                     VGSTTGAISDINGVYTISGLSENVYKLQLDYIGYQSHFETDIRVVRGKITYVREIEMQ
                     ETSIAGEEITVEASGFEQDKSAPVSNYNYSREEIRRSPGATGDIFRAIETLPGVSSSG
                     GEFSSFSVRGGSPRDNIVLVDNIPFSKVSHFDGGTEEQEAQGGRFSIFTPGLIESANF
                     QAGGFGARYGGKNASLVDLHIKEGNRETPTVNGTYDAIGWEINYDGPSYIHKKTSLLL
                     SARHQDFNNILKWTGQKDLGHPRYTDIIVKTTTDIASAHKLSFLGIYADDLFDRNLSH
                     VYESNNLYGNDLAEGGETKYLIGLNWRWLTGKKSFMESVVYYGHRENPFKSGVAYTDA
                     TNGIAPAQSEVRTRDNIYVYDFNEQQLGAKSQFTYQVFTHLTAMTGLEINRFSMDNTL
                     KQNGWDTTFVYDKDDYRSDISQLYLVRDPARTSYANKQTRYQSAGFLELSYSPDKKIT
                     LNPGVRYEYSTFNRRHAISPRVSISYVLNARTTLNGATGLYYQAPELDVTGSDARNKS
                     LKNQKALHLIAGGTRYLGNDVKLTIEGYYKKLDHLIVKPDRTSNLRTNQGEGWARGVD
                     IGLVKRFAQRWYGQINYSRMASRRNDHNGEGWYNSDFSQPNIFNVLFGYEFNKEWSFS
                     TKWKYATGRPKDTYVIHENVFNDTDHMRYSKEIIRNNGDRLDDFHTWNVRVDYRKQIG
                     RFAVVSFVDILNMYGHLNVNEEFFQYQNGKLDKRGFRVAPTMGVKIEM"
     gene            complement(8779..9711)
                     /locus_tag="PK937_02380"
     CDS             complement(8779..9711)
                     /locus_tag="PK937_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH31451.1"
                     /translation="MYQYTCISPSRYCDMVFFILIYIIAPLLLSYSAVAQDSVSFVYT
                     NNTNGYIEACDCGEEPLGGLARRKFIFDNIRKNERAVIFVDAGDFLNQFGFSPQQDSM
                     TIRLYELLSYDAVNPGDNEFANGYDFFERIVSRSKLPFTSSTLWHDTRPIAQPYQIVT
                     RENYKIGIIGYTPFSAFRYFPKNKQPGVHSVNERERLNAVIKDIRSRVDILMVLSHAG
                     SEEDKLILSDFPDIDMIVGAHDQVEIEEPIVDKNRYIVQAGGNGTKVGKLTLYLKSGK
                     MINHTNRFYTLSENITEDAEIKKLITRFKKANKK"
     gene            9900..10406
                     /locus_tag="PK937_02385"
     CDS             9900..10406
                     /locus_tag="PK937_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31452.1"
                     /translation="MKKLVVLALLVMLATAVQTNAQMKAGSMRPALELNFGGLGLGYG
                     ASFEYGITDKISARASANISSYKTGGDEWSLIPIDAVGVYNVDDSKYAMLGVTWTTFS
                     VDPSIGKKESSSSFGVVAGGGYRYKLNDKISVNGEGKYRVITLESGAYKLSVAWYTVG
                     AGFSYALN"
     gene            10549..11229
                     /locus_tag="PK937_02390"
     CDS             10549..11229
                     /locus_tag="PK937_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31453.1"
                     /translation="MRVNLLALFLFMYVGVTSVFSQEEEEHTKQKMAFISGSFSYQSS
                     TNANEELTIFLEDAKIKTFSVLPKLGLFINTNTAIGAGVGYTYEKLEASDYDGELTST
                     TPLFSIEPFMRSYRPLTDDASIFVDASAFYRFGTQTFDYYDEYDEDNYSEKTEKTSYG
                     VAISPGITYHLTENIAMELVVGKLHYTKTKNKPKGAPDDVAFKSSSYGLKLDLDQASL
                     GVVIYLTR"
     gene            11516..11713
                     /gene="rpsU"
                     /locus_tag="PK937_02395"
     CDS             11516..11713
                     /gene="rpsU"
                     /locus_tag="PK937_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNH31454.1"
                     /translation="MITVQVGENEPFDKALKRFKRKCLQSGLMRTLRDTAHYVKPSER
                     RKADRMRAIRKQKQIQAEEGN"
     gene            11791..13362
                     /locus_tag="PK937_02400"
     CDS             11791..13362
                     /locus_tag="PK937_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31455.1"
                     /translation="MKKMMLLFLAASLTVSAQITRQVLKPVKVDIFKNGNGFFVAEGN
                     LSMSNSEAQITDVPRTAFGTLWVGAMDKNIQISGLRAIEEKFANRRDPASLYEILISN
                     IGKQVIFRQSGEKAIEYTGILERVIGEVSQPVIVLRTTKGTMILNTWYAMHAEFPESF
                     QNTFSDTTKAMALRIKTNSAANAARFQMVYFQNQIGWTPSYKIDLVDDKNAQIILSGT
                     VVNDIQDIEKADVNLVVGYPHFEFANTLSPLTTPMPISDFLSALGYDARRPDFDNYGN
                     RMMAAQVARYEMSAGQADAGNFTPVGGTTVEDLFFYNLPDFSLKKNERAQINIFSATV
                     PYQHVYEVTLPNAFNIQEGYLYDKTPARKEPYEVWHSIRLENKTAMPWTTGAALTMQN
                     GKALGQDILQYAPAKSTTHVKITTSPDIYVTEEERETERKEDQKKKDGYSYDLVTISG
                     EINLNNYKDKDVKLKITRPITGKLREATAEPKSKVNVKSGSAINPETTLTWEVNLKKG
                     DARKITYTYEVYLRR"
     gene            complement(13372..14382)
                     /locus_tag="PK937_02405"
     CDS             complement(13372..14382)
                     /locus_tag="PK937_02405"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNH31456.1"
                     /translation="MLKIITYLFVFTGISLSICSQDYYDTGDYTAMLTQAEETASADG
                     KKILETGRTMTLTNAEILPGSCWDYANAVFDRAGYVWGKRETVFKSVKAGPFVDIEKI
                     KNGDWLYYINHSYGDIEHSAIFVEWIDKENKLALMLSYGGENRQQPARYLPYDLRSVY
                     GITRANSKLGEDKPTPKPKTVTSTNVAPVTNAVSNTGNNTSVKANNSNATTNKNAAPS
                     ETQSTPAGGNTIDGMLVESLRIGTGVANMEITGVADTFSSSTTEKIYCWLRVNGGQGK
                     TVKVRWIHNGNSLGEVPLDIRSQSMRTYAFRTVSGRKGSWKVEIVDAQGRLLRATDFT
                     VN"
     gene            complement(14580..15014)
                     /locus_tag="PK937_02410"
     CDS             complement(14580..15014)
                     /locus_tag="PK937_02410"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNH31457.1"
                     /translation="MEHTIKYPHRIGGWLWLLGLFLLINPIRILIALKQVSLPAANVL
                     ASNYLAYAEVTVTLCYLVYAIMVPIYFFRMDRRTPIMIMMLLLVNLVFVGLVGTSAQW
                     LSSDTNHNASSVRLAEFVIGAMMFIVWTLYLMRSERVKDTFN"
     gene            15251..15916
                     /locus_tag="PK937_02415"
     CDS             15251..15916
                     /locus_tag="PK937_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31458.1"
                     /translation="MVTRILAPAVVGLTDTVKIYTYDADESTLQIEAKIFDGDGQVLS
                     DVWKTNLHDDGLAGDMKASEGNYIGVIKSQTLLDHNSGFFELQVIVTEAKSGATDKQS
                     VFIEQNPNRTHPPEVILVTAPDTVNPSLVSEFLITVTVSDPDGLGDIASVTRTTPSNL
                     VLALRDDGVNGDATAHDGVYSERVSVNPSPPAGSYTFTFKATDRIGLQSAAIPKTIVI
                     ISN"
     gene            complement(15974..16183)
                     /locus_tag="PK937_02420"
     CDS             complement(15974..16183)
                     /locus_tag="PK937_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31459.1"
                     /translation="MNARSDCKHIRTKTAYVTEPDHHDAWRTDASATAQYWCLRTMLT
                     SGPDGSYVAPETCQHGRSCFEDVTL"
     gene            complement(16189..16617)
                     /locus_tag="PK937_02425"
     CDS             complement(16189..16617)
                     /locus_tag="PK937_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31460.1"
                     /translation="MAKNIFSVIGGIITVIVMVTIVQNVGATIYPPPADFNPNSVETL
                     EAHLRTLSLGAFLFAVAGMALGSFMGGLVASWIAPENGYRNAIIVAAFYVVFGVIGML
                     MISHPIQYWVANFITYLPCALLGHITLQRAVNKKNQELNF"
     gene            complement(16658..17647)
                     /locus_tag="PK937_02430"
     CDS             complement(16658..17647)
                     /locus_tag="PK937_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNH31461.1"
                     /translation="MNKFEYLQSASLDKLLPVLQEPDTAIKAGGVDIIDLMKEGLTAP
                     SRLVSLRPITALQKISLDDDLSIGAGATIADISNVNNIYAGPFRVLAQACASLATPQI
                     RHAATIGGNLCQRPRCWYFRSHEFDCTRKGGKICYAMNGENAYHAIFGNTDGCVIVHP
                     SSPAVALTALDARLKIKSSKGERELAMDQFFITPANDITKENALQPGEVITEIYIPEP
                     PNGTISFYLRQNEKQSFDWPIAEIAVALAIENNICRSARIVLGAAAPIPWRCTDAENL
                     LIGQTITKESAACAAKKALSNATPLSKNAYKISIFETVIRRALCFAVGIDPLR"
     gene            complement(17690..19975)
                     /locus_tag="PK937_02435"
     CDS             complement(17690..19975)
                     /locus_tag="PK937_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNH31462.1"
                     /translation="MPKNIKLKTGHSGVWTEKNVSLPDNVPKPWDADDRLQLVSKNQT
                     RLDGQAKVTGSARYTFDIQLPDMLHARILRSPHASAVIRKIDTSRTEKYPGVRAVIRI
                     QDTIPQFVRFAGQEICAVAATSTHAANEALKLIEIQYEKRGVITTIQDAMKPGASHIF
                     DTAVEEQKSEGDEPTSIGSVTQKGNLRGPAVKGNAASVDAALSSSAVIVDAVYETQVQ
                     THSAMETHGVVAFWENSDKLTVWASTQGTFSVRDELATVFQLPKTHVRVITEYMGGGF
                     GAKFGAGVYGIAAAKLAKIAGAPVRLMLDRKEEHIAAGNRPSAVQKLRVGANARGEIQ
                     AIKLIAHGTAGVGTGAGCDGPAMNLYFAVPHKYSEQYDVFTHAGAGAAFRAPGHPQGT
                     FALEQSVDELAYKLNMDPLALRRINSKTDSVRLAEYDIGAKRFGWDRRAAQPAGDKGP
                     IKRGFGMANAVWYYIYGTGFQATIEVHSDGSVDLTNGVQDIGGGIRTALAMIVAEELG
                     LQTKDIVIKIGDTQYGLGPASGGSQTTAGITPAARHAAYLAKLKMFEIAAPMLGVPSD
                     DLSVADGRIFSRRDTSKSLAWKQVASKISGDKFSVIGERARDYLTLSPPYIAGVQFAD
                     IEVDTETGIIKVNRIVAVHDCGRVINRLTLESQIRGGVIQGLSYALFENRILDAYTGR
                     MVNANFDQYKIAGAKDIPVIETEILDVYTGASATGAMGIGEPATIPTAAAIANAFFHA
                     TGKRIRKLPMHAAHVLEALQS"
     gene            complement(19994..20632)
                     /locus_tag="PK937_02440"
     CDS             complement(19994..20632)
                     /locus_tag="PK937_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNH31463.1"
                     /translation="MKEPQDDAGELPDPSRRAFLKDFGLSSLGAVAANGGIIGALQAS
                     CANPTQDIQTLGPGATDITLSVNGKKQSLRVEPRWTLAEVLRDHLHLTGTKTGCERGA
                     CGACTVWMNDKTINACLTLAVDAIGSEITTIEGLSDGMSLHPLQQAFIEHDALQCGFC
                     TPGMVMSCAALLRDNKNPSIEDVKDAVSGNLCRCGTYNHVFEAVQSAAKNMK"
     gene            complement(20732..21196)
                     /locus_tag="PK937_02445"
     CDS             complement(20732..21196)
                     /locus_tag="PK937_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31464.1"
                     /translation="MAIHIRLATVSDTDILVHNNAAMSKETENKELPRHILRDGVTNL
                     FRQPQFGFYLLAERPDGTVVGQLMITYEWSDWRNGVFWWIQSVYVDPEFRGMGIYRKL
                     YQYVLEQARTKKDVCGIRLYVDKSNRKAMNVYHRCGMNEAHYAMYEVDFVLG"
     gene            21337..22095
                     /locus_tag="PK937_02450"
     CDS             21337..22095
                     /locus_tag="PK937_02450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH31465.1"
                     /translation="MKISVIIPAYNEASIIGETLSALMKADRPSNPIEFIVVNNASTD
                     RTAEIASSFPDIRVVTESEKGLTKARQCGNAHAQGDILIYIDADTRIPKHLLRTIEEK
                     FIADPKLVAMSGPYKYHDWTWYGRLILWLYHWTLVPMNQLVINRILNKGSVFYGGNFA
                     VRKKALDKIGGFDTGLEFWSEDTQIGRRMSQEGRVRFFHRAYVYTSARRYYAEGFLRV
                     LMRYIMNYFWDIIFHRPFTKGYQDVRSTPPKQTT"
     gene            complement(22109..23299)
                     /locus_tag="PK937_02455"
     CDS             complement(22109..23299)
                     /locus_tag="PK937_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNH31466.1"
                     /translation="MIKKLFLFVSLCLTTAIWGQQIQYLHDEFSALPGDSLAEMEVWN
                     QVNIERTKAKLPPLVWDKALRSAARQHSREIMTQGYFSHTSPNASLKTVSDRVYRTGT
                     SDASLGENIALHSVDGGYRSIADQLMKQWMNSPGHRANILKAEFTHIGIGIASYKDSV
                     IKDTLIRGAKTRLARYTIRHYGTQVFCERDLMMDSLFIEKRSDDFLVLDLQFQSDRSM
                     LVAAGPRVQFVNAIGNSVPIHIEIPYEPGMILTLARASNEQTKEYALFFQDTVKSVLS
                     LTQALSPSPVLLLRKDIAITRKERWFLRGTARLMHPTLATACHVYINKDGYYIASKRG
                     DRIEWSIPIEADADTALYAFAVGKEIQKIITNQIYINTAVLDYPDTNPAEKARSVFVK
                     QITP"
     gene            complement(23306..23920)
                     /gene="ruvA"
                     /locus_tag="PK937_02460"
     CDS             complement(23306..23920)
                     /gene="ruvA"
                     /locus_tag="PK937_02460"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNH31467.1"
                     /translation="MITFVQGLVAEKEPTRAVIDVQGIGYELFIPVSTYEKIPAVGAA
                     CKLLTYMHVREDIMQLFGFVSAEERSFFLLLISVSGIGPKSGISMMSGIAIRDFKAAV
                     LNEDVQLLTAIPGIGKKTAQRIVVELKDKITKTGDVASTAKLSTSSANLQVADEALMA
                     LISLGYSKPVAEKAIARLLREAGDKPFALQDMIKQALRLASTGA"
     gene            complement(23923..24639)
                     /gene="radC"
                     /locus_tag="PK937_02465"
     CDS             complement(23923..24639)
                     /gene="radC"
                     /locus_tag="PK937_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNH31468.1"
                     /translation="MGKPKKIAEPSLYHAKIKDWSSQDRPREKLEQLGAARLSSAELI
                     AILIGSGTRNITAVDLARALEKEFSGLAALARCSTHELRRFKGIGKVKSIKLAAAFEL
                     SRRIEAQSDAKKIRVRAPEDLFKHYMPRLRDLNREIFMVVLLDSANQIIRDCTISEGT
                     LNASLVHPREVFRAAIEYMAASIILVHNHPSGNPEPSREDIAVTEQLKEAGIIMSIPV
                     RDHIIIAGRHYTSLAKSGYL"
     gene            complement(24655..25428)
                     /locus_tag="PK937_02470"
     CDS             complement(24655..25428)
                     /locus_tag="PK937_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31469.1"
                     /translation="MGNTVKFGDWISRGFDLLRKDWGTWALMGSLYFGPLVVALIMLY
                     ASIFALPFLGKHMDPDVALPGMVMFGLLVLGIGLFMLFLLLGMYLTARKQLKGESISV
                     SDAFSGGGHFFTVLVICLVYVFAACIGFVFCVFPAFIVGGFFYLAIPIAVYEGLGPME
                     AIQKSFSIAKKDWLMFTLFALVAQIIAQVGLYACYIPIVFSIGLQFTFAAVAYEDCVN
                     PAAANDAPTFTGAVKYCTTCGKQLRSETNFCDNCGAAQV"
     gene            complement(25443..26240)
                     /locus_tag="PK937_02475"
     CDS             complement(25443..26240)
                     /locus_tag="PK937_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31470.1"
                     /translation="MNPNYQVRLGKWLSEGWTMLRQEWMAWTGISALTFIPAGFVYIM
                     IQMLIGRSLQIGPNESLTDYFLRSMESSFAVNMLTQFAMVFIIAFFMGGAFRAAFRQI
                     QGESISVSDAFSGVDIYVSVLVAMLSVSLFSMIGSMLCLFPGLIAQGLLYYVVPMVVR
                     ENLPPLEALKKSFETTKSDWVMYTVLWIVTQVLAFLGILLCGIGIFITAPLIVTTTAA
                     AYRDCFEIKTEEPISRVDALYSKSCIRCGASIDVRASFCDKCGASQV"
     gene            complement(26330..27616)
                     /locus_tag="PK937_02480"
     CDS             complement(26330..27616)
                     /locus_tag="PK937_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNH31471.1"
                     /translation="MFSTVESLGHSQIMFFGIPFEFTLFALTLLGVALFHHYVLQVAL
                     IGLASILTFKLGFTNFNLMNHLHHEWEILTNLLGLLLGFALLAKHFEESKIPEILPKY
                     LPSGWMGGFVLLFIVMILSSFLDNIAAAMIGGTIAMVVFKGNVHIGFLAAIVAASNAG
                     GAGSVVGDTTTTMMWIDGVNALDVTHAFVGSIASFLVFGFIAAKQQHKFQPIVAAHAE
                     DIAIDWGKILVVFLILVGAITTNFMLDFPAVGVWIAILIGATFRKTAWHELPGALKGS
                     IFLLSLVMCASMMPVEELPPASWETAFSLGFVSAVFDNIPLTKLALTQGGYDWGILAF
                     TVGFGGSMIWFGSSAGVALTNMFPKGKSVVFWIKDGWHVAVAYIIGFFIMLYMVGWHP
                     HAPHKEGKETKPHAQVIEQAPAGSCCMPGAGGTSSH"
     gene            complement(27771..28322)
                     /locus_tag="PK937_02485"
     CDS             complement(27771..28322)
                     /locus_tag="PK937_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNH31472.1"
                     /translation="MEPIHKPFRLSDLKPPEIHDSVFIANTASVIGGVKIGENCGIWF
                     GAVLRCDMDPIIIGKNCNIQDNAVVHVDDHFPAIIEDNVSIGHSAIVHGATIRKNCII
                     GMHATILNGAEVGENCIVAAGAIVMQNQIIPPNSVVMGVPAKVKKTADEQTLAMIRRN
                     WEVYCEFAAEYRKSKYHKDKSLL"
     gene            28493..28900
                     /gene="cdd"
                     /locus_tag="PK937_02490"
     CDS             28493..28900
                     /gene="cdd"
                     /locus_tag="PK937_02490"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNH31473.1"
                     /translation="MNEREEIEALIQEALHARSFAVAPYSKFQVGALVVMKSGKRFTG
                     CNVEVSSYGLTICAERTAIFKSLSEGERDVHTVVVVADTTGPCFPCGACRQVLSDFAP
                     NARIICSNIHGVYSVFSMKELLPHAFDANDLHP"
     gene            28945..30033
                     /locus_tag="PK937_02495"
     CDS             28945..30033
                     /locus_tag="PK937_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNH31474.1"
                     /translation="MAEWFGVFQVEDPVWRHLIQAGVIIVGGYVLAKLLYRLVHIIGY
                     KISAKTETDLDDKIVAIFEKSVQRIVNTTALYLAINRLEPLFQGTWLNYVDGAFFVVM
                     VILATILLTSVTKTIIEWYVATIAVRTESPLDEEILPLIRRLLNMLLYSIALMICLDH
                     FHIDIKALVVSLGVGSFAIAFAAQETLSNMIAGFVIMVDRPFRVGDRIRITAGNITGD
                     IVKVGLRSTQILDFDYNIVTVPNAQIVKSDIINFAYPDPAMRLRIEVGIAYGSDIERA
                     KKILLEVLQSDERILKEPLPEVAVTGFGDSAVQLVAIGRVRHYKEHFETGDTVRDRIH
                     SSFARNGIEIPYPHRVIIARTDQNAKNG"
     gene            30195..33998
                     /locus_tag="PK937_02500"
     CDS             30195..33998
                     /locus_tag="PK937_02500"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH31475.1"
                     /translation="MLKVRLTGIAVWAAVLLSFFAQTAQSQSNARGMFEFVSRVGNPD
                     DGATDLVLDGKTLFVANKFSGLQIIDISDIRRPIIRSRTKSEGQNFGIAKKDKMVFLS
                     DNKAGLVIYDVTSLSKPKLVTSFETRGEAWDVQIKDNYAFVAAGLAGLLVIDITDPKA
                     PKEVGSLRFDKDWDFARQLLIRENRLYLADRKAGVHIINIENPLAPKEIKRFPTQFAE
                     GLDVWDNYICVADGPSGFVILDITNPEKIRQIAAQSLPGYANRIFKEGPYVYIGVDDA
                     GIRAYDVSNPAKPFFDARYDTPGQTFGMIKYDIFLVAADLNAVLFLTHNKPPVITRSG
                     DKVIAENQTLRFKIKGMDPDNNPIGFSANFIPQGASFDIKDTVFSWTPSFEQSGVYEG
                     IVFSATEKTQSALFSRDTIKITVNNVNRAPALPSTGNYTVDENKELAFTINKPSDPDI
                     EDTTNLKVELTNPPTGAIFDSKTLTFKWIPAYDQSGVYTLSFVVTDGGGATDTKQTTL
                     TVKNINRPPMFDALAASFTTDENKPVSFTIVASDLDKEDAGKLEWGGFNIPPGATFDR
                     SSGVLTWTPSYDQSGKYDGIYFIVRDAEGLSDTVNTSITVNHVNRPPFFATVDAQKID
                     EVKPLSFKIAAADADAEDDGKLTIKATTVPQGAIYNEITRTLTWTPTYDQSGDYTAVF
                     KVTDPAGASDEMTVPVQVVNINRAPSIAAVPAQNADENKAYTLVVTEGTDPDKEDVGK
                     LTYKAENLPQGATFDPATRTIAWTPTYDQSGVYEGVKVTVTDALGLTATTTFRFTVKN
                     INRPPVMEVIAAQTSDEQKPLTFQVKFSDPDKEDANRLTVAAAGMPLGASFAGGNFTW
                     TPTFDQAGNYTVKFTVTDFEKLSDTKEAVITINNVNRPPVLAAILAQAATENTPFTFT
                     VPAANDPDKEDAGKLTYKAEGLPEGANFDAATRVVKWTPTFDQSGDHNIRITVTDVLG
                     VADNKELVIKVKNVNRTPVVTDVAAQNGDENAKMEFRVETSDPDKEDADKLKVVAEQM
                     PQGATFANNTFSWTPTFDQAGTYRVTFRATDVAGGFAVKTAVITIKNVNRNPVLGAIN
                     NQTADENKFMSFTVSASDPDKEDQIRLNVTASDLPKGATYAAGKFSWMPGYDQAGDYT
                     VKFTAKDPNGGEDVKSVSIKVNNTNRAPKMNAVGTKNGKEGSEISFSASASDPDNDNL
                     SFSLVGGPSGATMSSDGKFTWTPQTGQSGTFNFTVKVSDGTAEASAPVTVIVAKAPQP
                     QGGGQ"
     gene            complement(34302..34784)
                     /locus_tag="PK937_02505"
     CDS             complement(34302..34784)
                     /locus_tag="PK937_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNH31476.1"
                     /translation="MKRFMILGLLMVVGLSTAFAGDPDAIIGKWYNEEGTAQIEIYKQ
                     GSQYFGKIVWLKEPTYNANDVNDNEGEPLVKLGAEKVDFKNPDSGRRSQKIIGLVILR
                     DFTYDSAEGEWSNGMIYDPKKGSDYKCYMQLMEQNKLKIRGFIGFSLIGRTSYWSRAN
                     "
     gene            complement(34809..36527)
                     /locus_tag="PK937_02510"
     CDS             complement(34809..36527)
                     /locus_tag="PK937_02510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH31477.1"
                     /translation="MSTFKEYSLSPEVQKAIEEMGFVTPSEIQSKAIPLLLSHHGDFV
                     GQAQTGTGKTAAFGIPLIEKIDPSAKTVQAIILSPTRELAVQISEELKKLSKHKRLRI
                     LPIYGGQDIGVQLRALRQGVQIVVGTPGRVVDHLHRGTLQLSQIKHFVLDEADEMLDM
                     GFLEEIEEILTHAGTERSIWLFSATLSGQIRGIAKRFMTDPREVKIQKNTATTENTEE
                     IYYAVQEHNKFEALCRLIDHAKEMYAIVFCQTKAQCAEVAERLVLKGFKAEALHGDMN
                     QAQREHVMRKFKAKGVRLLIATDVAARGIDVNDLTHVINYSLPMESESYIHRIGRTGR
                     AGKTGIAITLVTPSENYKLRRLEGNTRKRITKSTIPGIQEIMEANIDRTITEFEEALK
                     NPAGFDRYFDRWKPIIKKYETEEIMRGLVTLLCREFLEKYEDDKELNVPDNFGQRSGM
                     RDRSYAPRAEMTELRINVGAQHNLQTGTLVGKICNMARLEGRQVGKVFINEDHSVFRV
                     DAESVDRVIRSLTGITIGGKKAVVERTDGRSGGAPRMPFAKRSSHAKEDSPFRKSRTY
                     KKDHSS"
     gene            complement(36626..37588)
                     /gene="lipA"
                     /locus_tag="PK937_02515"
     CDS             complement(36626..37588)
                     /gene="lipA"
                     /locus_tag="PK937_02515"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNH31478.1"
                     /translation="MSRQYPMTSVQVDYIKKKTEEVQEKEPLKRPEWLKVKIPIGEKF
                     SEIKNLVGSKKLHTVCEEARCPNLSECWNRGTATFMILGDTCTRSCGFCAVKTGKPTW
                     LDKDEPRRVAESVRTMNLRHAVITSVNRDELADGGSMIFAETIRAIRKAIPTCRIEVL
                     IPDFKGNFENLKNVIDAHPDILNHNTETVPRLYQVVRPQANYAQTLEVLDRSKKNGMI
                     TKSGLMLGLGEKNDEVLAVMDDLRKVDLDILTLGQYLQPTKEHIPVDRFVHPDDFAMF
                     REKGMAMGFRVVESGPLVRSSYHADEQTDVVADSFVRRDSVQIL"
     gene            complement(37705..38316)
                     /locus_tag="PK937_02520"
     CDS             complement(37705..38316)
                     /locus_tag="PK937_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31479.1"
                     /translation="MKLFIMFCILFTFIGVAMVTAQQPQGEFHKYIIKKTDDERRKTK
                     GQEWWGAGAVVFSKEEIPRLGKTAEVQPLLQTQFNHGDTFFGRVYSPVHMGGLPGGMP
                     EVIEYRVIENGKRIYEIESRGDALPESDWSSWLMELPSDLQRCFDELPEGTHNLRVEV
                     WSARTVEVLTEWKDRETDKTVGFTKENEGRIKFIAAGDIVYVK"
     gene            complement(38369..38713)
                     /locus_tag="PK937_02525"
     CDS             complement(38369..38713)
                     /locus_tag="PK937_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNH31480.1"
                     /translation="MNDCIFCKIADHRIPSKPVYEDSDVIAFHDLHPKAPTHILLIPK
                     KHIEKLSDMTGDDSELGGKLLLAVGKVAEAAGIKDNGFRVVVNNGKYAGQEVFHLHLH
                     ILGGKPMGWNPA"
     gene            complement(38723..39643)
                     /locus_tag="PK937_02530"
     CDS             complement(38723..39643)
                     /locus_tag="PK937_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HNH31481.1"
                     /translation="MISDSLARSLNQEQHLDWHASVTIRIAILDMNNGLRNLGIRNIK
                     RIIDNFTYYVRVNGYAVDFQVDHFHVRDRNEVPENGYDIYISSGGPGSPLEETATDWE
                     EKFSTLLDQILEHNLSHENKQYFFGICHSFQLMAKKFEVGYISQRDKRNLGIVPIFRS
                     EEGRQDYLFEGLQDKFYAFDNRDWQVTEPDTQRLKNMNARILSFEGSESHAGKAITGI
                     RYSDEMESVQFHPEAEKSAILMRFTDPDEKSHIIEVLGDKAYEEFIQSVLNPNKLLRT
                     YKAILPGFLRRSFNGLMNYYELPHLQKPIA"
     gene            complement(39648..40451)
                     /locus_tag="PK937_02535"
     CDS             complement(39648..40451)
                     /locus_tag="PK937_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNH31482.1"
                     /translation="MEETTSHTTEAGPAGPPQSSPNGRSFFRSNGLILALVAVSIALD
                     FFPFTGMILLPFEFFTTFIHESGHAIASLIMGESVSRIVINPDTSGYMQHTVSGGRLA
                     QGFIASAGYLGAALFAGILIIMSGRRNLARVILAVIGIGFAVAIVFYVRDFFTLAVSG
                     GLCAVLLLLAFKGGTWLNYIVINFLAVQCALNAVSDALTLVRLSMGATRSRYSLGHSD
                     ADTVAQLFFLPAIVWSILWVIIAVAILYYALKISARFQASAKPPQPSAG"
     gene            complement(40568..40951)
                     /locus_tag="PK937_02540"
     CDS             complement(40568..40951)
                     /locus_tag="PK937_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31483.1"
                     /translation="MKNHTLLIVMIVLMCIACKHTGYLIAADPALKNIEGFWSDTQRK
                     TCWIFEKNGAFQYGRQDSVVHVFMCGQYHISSDELVMTDRLGNRLVYDFFGQADTLWI
                     RSGDEAIRLLRSRPMRIINACPDDQ"
     gene            complement(40948..41610)
                     /gene="hxpB"
                     /locus_tag="PK937_02545"
     CDS             complement(40948..41610)
                     /gene="hxpB"
                     /locus_tag="PK937_02545"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016350848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HNH31484.1"
                     /translation="MIKSVIFDMDGLLINSEPFWRQAEREIFADLGIALTDADCERTM
                     GWRFDEVVHYWYKRKPWSGVDLYTVEQRVVDRVIALSTTHGKVMPGVYHALDFFTKHV
                     GKLALASSSAMRIIQAVLTHLKIKDRFEVIHSAEYEPYGKPHPGIFITTAERLNVNPT
                     ECLVIEDSLSGVIAAKAARMLAACVPDTAAAGKPQWCIADIQLASLDAWDDKTWAKIN
                     AL"
     gene            complement(41622..42290)
                     /locus_tag="PK937_02550"
     CDS             complement(41622..42290)
                     /locus_tag="PK937_02550"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH31485.1"
                     /translation="MTPDEVITLLCQAHENEARQPLAHVPPDLIQSRIKLALLKKIQK
                     KISNDAALLENLWNTNIPEAQILVAMTSHHTSPDINLLQHRIETVQHPHVADALIESI
                     TPDLTDRKQWIDRWTSSPKEMVRRCGYTILALSGPAISEADCMAYLWNIVRDIHESPN
                     FAREAMNKAVIAIGQTSPRLNKEALFAVTQIGPVVLDHTDAPDALKILTDKKLQDKFK
                     KSRL"
     gene            complement(42336..45533)
                     /locus_tag="PK937_02555"
     CDS             complement(42336..45533)
                     /locus_tag="PK937_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31486.1"
                     /translation="MNALLVKSVHLFFFVCVFVGLDFSYALDPARRLTQYAHTNWHLK
                     EGLPQSTVTSIVQTPDGYLWIGTFEGLVRFDGIQFEVFNKTTEPVFISHSIISLTVMP
                     DSSLWIGTGGGGIVIHKNNRFYSTASFSPLSERFVNAITMDHDKRIWIATDRGLYHTN
                     GDSLSRVFLPYNSSESIIHALQCDLNGNIWVGSHDGLYMINSISKKIIIVDSINKNIS
                     NKIAIHALSINKDNRILVGTNNGFFGVEQTDQSYGLKSYSEFDIPNNTQIYAIRYDRH
                     GNLWLGSSLGLFRWNQKHMELISAKEGLMNNTIISLYEDREGIFWFGSESGGLSCFKD
                     ALFITYGNEEGILDNVIQCLFVDRKNRVWLGTDLGVSMLDFGNQNTRHTSLHLGTNGL
                     PNFPIHALYEDPEGAMWIGTDGGGLTRWSDGKYKLYNKTNGLTNNFINVVLKDRSGIV
                     WIGTKGGLHALHHNNISVYTTFNGLNHDIVRALHEDRKGGLWIGTGSGVNILRNGKFE
                     SFKSPDETVSHSVMTFYEEESGDMWIGTHGGGIIRYHDGHFKRYSSRDGLFDDVVFSI
                     LEDDSANFWMSCNRGIFRINKHQFEQLDRRIITKLESHAYGIDDGMKTNECNGGAQYG
                     GIKDKSGRLWFATINGVSVVDPSKIVKNFDPPYVVITRFADAQQNIRLDSTIFIPAGH
                     DRIEFYYTGLSYSAPGKIRFKFKLDGYDNDWIDAGYRRTAVYTNIPPGTYRFQVIAAN
                     ASGIWNTQGASLDFVQQPFFYQTWWFVLLCLTGVVFVVLGAYRYNVRQLIAREQQLAA
                     LVQMRTRKLEEEKERTENALQMAESARRVAEREREIAQQASETKSQLLGIAAHDLRNP
                     LQGIVGYTDLVRVELNNPEKANKDLDQIQRSSSQMLHLINGLLETMSIESGKLKLNKE
                     KTDVSEITALVIQDNLIHAEKKAQRIHFHPHADSYVWGDAIRLRESFENIVNNAIKYS
                     PRDSEIDIKVEATDTIIRFSVRDRGPGLTLEDRRKLFGKFQRLSAKPTGRESSTGLGL
                     AIVKQLVELHDGQISVESTPGEGAVFIIELPRYNG"
     gene            45678..47021
                     /locus_tag="PK937_02560"
     CDS             45678..47021
                     /locus_tag="PK937_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNH31487.1"
                     /translation="MSLFEIDKSGFEQHTARVPLAERIRPRTLSMVIGQEHLLGHGKA
                     LRTQIEQGNVVSMIFWGPPGTGKTTLARVIAAETRSKFVSLSAVEAGVADVRRVIQDA
                     QALKRQTGERMMLFIDEIHRFNKSQQDALLHSVEEGTITLIGATTENPSFEVINALQS
                     RCKIYKLESLTDAHLEKILDQALKTDDLLKTLTVEIADLHSLIFYAAGDARNLLNTLE
                     LAIQITRPDTNGKHIVTREIVEEAYQQRNIHYDRAGEYHYDTISAFIKSVRGSDPDAA
                     LYWMARMLEGGEDPKFIARRLIVLASEDIGNADPYALTLAVSTFTAVDYIGMPEARIV
                     LAQCVTYLASCPKSNASYLAVDAAIHDVRSEKSHPVPLHLRNAPTKLMEKMDYSKGYK
                     YAHDFSDQGEPHFVEQIYLPDDLKARIYYQPTANGGEKRLKDRLEKIWKKRNKKD"
     gene            47025..47456
                     /gene="dut"
                     /locus_tag="PK937_02565"
     CDS             47025..47456
                     /gene="dut"
                     /locus_tag="PK937_02565"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNH31488.1"
                     /translation="MPVVKIQKLHPDAKLPSYAHPGDAGLDVCSVERVEILPHASALI
                     KTGFCMQLPDGTEAQVRPRSGLALKHQITVLNSPGTIDHGYRGEVGIILINHGSTPFI
                     VEPGMKIAQMVIAEVLHAHVEEATELTDSSRGQGGFGSTGR"
     gene            47459..48154
                     /gene="lipB"
                     /locus_tag="PK937_02570"
     CDS             47459..48154
                     /gene="lipB"
                     /locus_tag="PK937_02570"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNH31489.1"
                     /translation="MSNLFQIHRLGLVDYQVAWDLQKQLFDDCLSGRNGDAIIICEHP
                     HTYTVGKNGVESVGKHLLLNQEQLRENGILVFEIDRGGDITYHGPGQIVGYPILNLNR
                     YYRDVHRYLRDIEEVIIRTIRHYNIVGKRIEKVTGVWVDTPRGPEKICAIGVKVTRWV
                     TMHGFALNVNTNLDFFNGIVPCGITDKGVTSIAKLLNKEQDIREIENVIIHEFQSVFK
                     AAEMPIMENQGAI"
     gene            48151..49035
                     /locus_tag="PK937_02575"
     CDS             48151..49035
                     /locus_tag="PK937_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31490.1"
                     /translation="MKKYIFCYVVIISEVLGRWGLAQNPDQAKSWYDKGRSAYLRFMS
                     DDYKTALSYYERSIAADSNYAPAFAGKAEALTLLALEKEQNGLAAEADFTHALRSAQQ
                     AIAKKPDQNLGYRALAQVYLNMDSKTYGEKAHDALQRAIELDSTDAESYFLLWLLTEN
                     DKPNSPLLRLSLKLDPNSFQTHYALGQYWSRNKQPDKAIDEYKLCTRINPDNYRSYYS
                     LGNVYSQIKRYDMAAEEYEKAIAINKNLYEAYFYVGVAYYYLDKNKKAKQRLSQYIEM
                     QPNPKYKLQAEQLLMDMK"
     gene            49512..>50594
                     /locus_tag="PK937_02580"
     rRNA            49512..>50594
                     /locus_tag="PK937_02580"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13866 a        11127 c        11801 g        13800 t
ORIGIN      
        1 gaccatcatg gatataaagg cgcgcgcgca aaaaatgccg aagcccgggt tttttggaaa
       61 cagggttatc caaacggacc ggaatgcgca aaatcggcga cggttgactt gccatgcgcg
      121 ctattgccgg cgtcacatac agttcgaagc atacaagcga actcacgggg tttcccggta
      181 atccgaaaaa caattttttt ccttttgatc cgaaaaccat aggttttccc ggtttttgcc
      241 ggactttcca aaagtgaatt tttatcccag ctttttctac tgcttggcgt acataatctt
      301 tttcgcccat ggatacgccg cccgttgtga tgatgacgtc tgcttttaat gctttttgta
      361 tttgagcggc gatggctttg ggtgtatcgt gcgcaattcc cagattgatc accgggtaac
      421 cggctttttg taataaagcg gtcaaggcaa aatgtgtgct ggcgcgtatc ttgccgggtt
      481 ttagtttttc ttcaacggat aacaattcgt caccggtcgc aagcaatgcg atggtcggtt
      541 tggtttgaat gcgaattttg gattttccga tagacgctaa aacaccaaga tcataaggtc
      601 gtatgatgtg atcacgtgaa aaaacgatct ggtttttctg gatatcggat ccggctttac
      661 gaatgtggtc accttcgcgg gccggctttt gtatatgaac ccattggccg acgcgacgcg
      721 tatgctctac catgacgata gctgtcgttc ctcgcggtac aggcgcgccg gtcatgattt
      781 gaatagctgt tccggctgtc aatttttttg ttgatacatc gccggcatgg actgtgtcca
      841 aaacctttag catacacggt tgtttggttt tgcttgtatc ggaagcaatc acagcgtaac
      901 cgtcaacacc actgttatcc caaggtggta tcatttcgcg ggacacgata ttttctgcga
      961 gggcataacc gcaggcatcc ttaaatggca ccgttctgat cgcaagcggt ttagctttgg
     1021 ataatatgat gtgcagcgct tcatcaacgg atatcatagt cactccgtat aaattgataa
     1081 ttactgattt tcccgccgct gtcgcttttc agagttatcc gaaacaaact ttcctgaaaa
     1141 ccaccggtag catcccgatc gagcagtact aagtcataaa tcatagtacc ggtagggtcc
     1201 ggtatggaat ctttgacata aaactccgat atcgaaatct caggttcttt ccatatatcg
     1261 ggaagttgtc gttcaaaaga ggactcgtta agtattttag agcgagcggc atcggtacaa
     1321 tgaacaaggg cggcatccca ttttcgatga aaataggagc gcgtaaattg gcgaactcga
     1381 cgggacggtg aatcttcgtt aggttgggta caggtttgaa tcagcggaaa aactgcgatt
     1441 accataatcc aaaaaaatcg tatcaccggt atcccaaact ccggagcata gcatcgttac
     1501 tgcgccactc ttctttgacc ttcacccaca attctaaaaa aacctgacga tcgagaaagg
     1561 cttcgatatc tttgcgtgcc gtttcaccga tacgtttgag ggcttcgcct ttttttccta
     1621 ttaaaattgc tttttgtgaa tcacgttcaa ctatgattat ggctcgaata aggtctttgc
     1681 cgcgttctct ttctttaaat tcttcgatga tcacttccgt cgaataagga acttcttgtg
     1741 tgtaatggag aaaaattttt tcacgaatta tctccgctac aaaaaaacgc tcgggttgtt
     1801 cggtcaacgc atcgggcgga taaaatggcg ggttcaacgg caggtatttg aaaagcgctt
     1861 gtttcatctg ttccacaccg tcattttcga gcgccgatat tggaatgacc tctttgaaaa
     1921 atccggtctt gatatacgta tccatcatcg gaagcaattc cggtttggcc attcgatcca
     1981 ctttgttgat cgccaaaact ttggggatat cggagtgcag aagtttggtt ttaaacagtt
     2041 cttgtacaaa atcggactcg gcttttgaag ttccgtcaat catgataagc agcacatcgg
     2101 ccgcttccat agattggtcc gctattttca tcatggccgt ttgtaaagcg tattgcggcg
     2161 caatgagtcc gggggtgtcc aaaaaaatgg cctgtgtacc gggttcattg agtataccga
     2221 gaacgcgctg gcgagtcgtt tgaggtttgg gcgtgacaat gcttagtttg acatgcaaaa
     2281 gctgattcat cagagtggat ttgccgacat tgggacggcc gatgatcgct acatatccgc
     2341 agcgtatcat agcggatgtt ggctcgccta ggttgttcgt ttcattcatg atatcaacgc
     2401 ctcaggtaga ggtaactttc aaattcttga cgattcatta attcgtagtc tccgatctga
     2461 ttgacatcca cgaataataa cctcaaattg gcttggtaat aataccatat ttcatagggt
     2521 ttgtcattgt tatcaaaagg gtgccgttcg atatcatcgg gagcttccaa tataataaaa
     2581 accatgccgc gatccgtatc ccatccttcc cgatatccgg atgtgaaata acggttggca
     2641 taatccactc gacgaaagta ttcttccatg atttcgttgc gttccgtgcc gggggttgga
     2701 tctttagcgc gccagaagtt ttcaaaaaaa tcactttttt ctttttgcgt ccctttgaga
     2761 attttatctg tgttcagatt tcgaccgaca tgacgcattt gtttgatggc gacatccaaa
     2821 tcaaaggccg ttgccgataa cgacggtgcg gtaaacgtag acagccgcac gtcaaatatt
     2881 gctttggagg tagcgcggac accgttttta ccgtacacca tcacatctaa aacatatttg
     2941 ccgaagctca ctttactttt atcaatctcg atcgaactga acgtttgaaa ttgattgacc
     3001 tcacggacgg tggaatcttc cgttacgatc ttttgatttt tatcatatac gcgatagcga
     3061 atacgtacag gttctttttc ctccgggaga tttccgatgg agtcgcggtt tttccaggcg
     3121 tatttggaat tgtagatttc aaaatacacg gcatattttt taaactcatt atcaaaactt
     3181 ttaaagacat ttgggagaat gtgtatgata tcgtccacac tgtctttttg aattttatcg
     3241 gcaaaaagca gatcgcttat accgattgat ttttcacgaa atgtacgcac cggaaggctc
     3301 agtttacgct gaccgaattt ttgtgtctct ttatccgtaa tgacaacggt aaggatatat
     3361 tcacccggat caatattgag cgtcaggcga ctaaagtgaa accgcagatc ggaaagagtt
     3421 tcttcgtagt gctctgtaac aatctcttta tcttgtatct cacgaagcac gacgttgcct
     3481 tttttgtcgg ctaacgtata attgacttcg tattgcgaac ggaagagtac ctggttgtat
     3541 cgggcaaata acaattcgct gtatgcgatt ttgatgaaaa tttccacccg tcctttggtg
     3601 gtgtctttgg cggctacatt ggctgcatcc aaaaagaaaa aagggccgcc accggttttg
     3661 cccatgttcg cttgtccccg caacgcacag ctacctaaaa aggtgagcac gataaggctt
     3721 aaaattcgtt tcaaattgac cactccttga gcttagatga ggttatgata tatgtcccac
     3781 aatacatatc gtttaaaagt taacactaat ctttataacg cttcagcata caggtcgtat
     3841 tccgtggcat cggtaatttt aacatcaacg aattgtcccg gttttaattt ccgggatgaa
     3901 gtgataatta cttcattgtc tatttccgga gcgtccgaca tagtccgccc aatatagtgc
     3961 tcgctacttt gggtatcaat catcacacga aatgtttgat caattttttc catgttatgg
     4021 gcgtgagaaa tttcacgctg tatttccatc aaacggttaa agcgttcttt ttttacggct
     4081 tcttcgactt gaggttccaa atcatacgcg taggtgcctt cctcttgcga ataaataaat
     4141 acgccaagcc gatcgaactt aaattcgcgg acgaaatcac acaattcttc aaagtctgct
     4201 tcggtttctc cgggataacc gacgatcata gatgtacgta gggcgacatc gggcatgcgc
     4261 gctttgattt tgtatagcag atcggtggtt tgctttttac cggtattacg acgcattgtt
     4321 tttagtatgc gatcggaaat atgctgaacg ggcatatcaa tatatttgca gattttggga
     4381 tgtgttgcaa tggtttcgat caaggtatcg gaaaatagag acggatacaa atacataacg
     4441 cgtatccatt cgatgccgcg cacttcgccc aaagcatgca acagttcgat caggtgtttg
     4501 ttctgccgga gattgggggc gctgcgatca aaatcatatc cgtacgacga agtatcttgg
     4561 gaaacgcaga tcagttcttt aacgccgagc gaggctaatt tttcggcttc agcgacattg
     4621 tcggcgatgg atttgctgtc gagattaccg cgaatcatcg ggatataaca aaacccgcaa
     4681 cgctggttac aaccttcgga tattttgaga taggcgtagt gacgctggtt gagaagaata
     4741 cgttcggtaa acaaaacttg ctgataatgc ccttcgatac gctcggtgat tttgtcaaac
     4801 tggtgtacgc cgaaaaaagc atcaacttca ggcatttcag cggctaattc tttcggatag
     4861 cgctctgata aacacccggc aacaatgatt tctttggtaa ttcctttttt agcggcacgt
     4921 tttttgagtt cgatcgcttc gagtatagtt tgaatagatt cttgtttggc gctttcgatg
     4981 aaaccgcagg tattgatcac gatcgtatcg gcttcctcgg ctttatctac gatacgcgta
     5041 tgcttcaggt tgcccaaaat gacttcggag tccacttgat tttttgggca tccaagtgaa
     5101 acgatattca cttttttttg tttattggaa cgtggtgcgc gttgccttga agcttcgccg
     5161 agtaccggca gcgttatgcg ttttgatgtg gttgacatgg gttcctttcc gtcggccaac
     5221 ataaccaaaa cacctctgaa agtcaacgaa gatgggttaa agaaaaagca agaatttctt
     5281 ggctctatca gttccaaaaa aatcatttaa ccgatcaggt gtcaacgcat ttatgttagt
     5341 tttgattttt atttaacctt gatatccccc aacaaaatgg ttgaaagtat taatctagtt
     5401 gaaagtatta atctaataat tgagttttag gctcttttca aaaaaattac cttgacaatg
     5461 gatcgttctc atgttatttt aacagtgttt agtaaacgat gtttaattaa tattaaaatg
     5521 gcaagaccaa aagaaagcga aagacaagat ataaaagcat tggtgcttag gcattccaaa
     5581 gccttatttc tgaagcaagg ttttgcgaat gttactatgc gtgatattgc gcgagaaata
     5641 gggtatagcc cggcggctat ttatctttat ttcgacaaca aagatcaaat ttattacgaa
     5701 ctcttcaacg aaggtttcca aattttatat agacgtcaac tggaaaccgc ccaacaaagt
     5761 ttcaatgatc cgacagatga attgtacagc atgggtcaag cgtacatacg ttttgcgatg
     5821 gattttcccg aatattacga tctcatgttt gtgatgcggg agccgcgcaa atttatggaa
     5881 cattgcctaa atgaaaacac cggtgaaccg atacgaaccg actttagtga aatgtcttac
     5941 aatctgcttc gtgcgacgat taaaaagtgt attgatcaag gctattttaa aggttttgat
     6001 gtagagatcg cttcgtttac gacatgggcc gccgtacacg gtatggccac tttgattctg
     6061 cgtggaatgc tcgtagctcc cgaagaaatg aagccgatta tttttaatgg agcgcatgaa
     6121 tgtttgcaag ctctcattaa aacaagtaaa caaacaaaat aaaaattttt acctcataac
     6181 taaacagcgt ttataaaaaa ttaatagaca aatcattttt taatagataa ttacacataa
     6241 aaactaaaca tcattcagaa agggctatta tgaaacgtgt tattctcatt ttgagcgtat
     6301 tgtgtattgc gcatttgggc gcgcaagaaa aaggtaccat aaaaggtaaa gtgttagatg
     6361 ctgtcggtaa acaaccttta gcgggcgtat cagtgcgtat cgtgggcagc acaacgggag
     6421 ccattagcga tatcaatggc gtttatacta tttccggatt aagtgaaaac gtgtataaat
     6481 tgcaattgga ctacatcggt taccaatcgc attttgaaac cgatattcgt gtcgttcggg
     6541 gaaaaatcac ttatgtgcgt gagattgaaa tgcaggagac atccattgcc ggggaagaaa
     6601 taaccgtcga ggcttcgggt tttgagcaag ataaatcggc gccggtatct aattataatt
     6661 attcacgcga agagattcgg cgcagtcccg gagctaccgg agacattttt cgtgcgatcg
     6721 aaaccttacc cggtgtgagt tcgtccggtg gtgagttttc aagtttctct gttcgtggcg
     6781 gaagcccaag ggataatatt gttctcgtgg ataacattcc gtttagtaaa gtctctcatt
     6841 ttgacggggg caccgaagag caggaagcgc agggtggacg tttttcaatc ttcactcccg
     6901 gtttgatcga gtcggctaat tttcaggccg gcgggttcgg tgcgcgttat ggcggtaaaa
     6961 atgcttcact cgttgatctg catatcaaag aaggaaatcg tgaaacgccg acggtcaatg
     7021 gtacctatga cgcgatcggc tgggaaatca attatgacgg accgtcgtat attcataaaa
     7081 aaacgagtct tcttctgagc gcacgtcatc aggattttaa taatattctg aaatggaccg
     7141 gtcaaaaaga tctcggccat ccgcgctata ccgatattat cgtaaaaacg acgacagaca
     7201 tcgcatcggc acataagttg tcctttctcg gcatttatgc ggatgattta tttgatcgaa
     7261 atctgtcgca cgtatacgaa agcaataatc tgtatggcaa tgacctagct gaaggcggtg
     7321 aaacaaaata tctgatcggg ctcaactggc gctggcttac cggtaaaaaa agttttatgg
     7381 aatcggtcgt ttattacggt cacagggaga acccgtttaa aagcggagtc gcctataccg
     7441 acgcgacaaa cggaatcgcc cctgcacagt ccgaagtgag aacgagggat aatatttatg
     7501 tctatgattt caacgaacaa cagttgggtg ctaaatcgca atttacatac caggttttca
     7561 ctcatcttac agctatgacc ggtttagaaa tcaaccgctt tagcatggat aatacgctca
     7621 aacaaaacgg ttgggataca acctttgtgt acgataaaga tgattaccgt tcagatatat
     7681 cccaattgta tcttgtgcgt gatccggcaa ggacgagtta tgcgaacaaa cagacacgct
     7741 atcagagtgc aggattttta gaattaagct attcgccgga caaaaaaatt acactcaatc
     7801 cgggggtgcg gtatgaatac agtacgttca atagacggca cgccatttcg ccgcgcgtga
     7861 gcatcagtta tgttttgaat gcgcgaacga cgcttaatgg cgctacggga ctgtattatc
     7921 aagcgccaga gctggatgtt acgggtagtg atgcgcgaaa taagagtctt aaaaatcaga
     7981 aagcattgca tctcattgcc ggtggtacac gttatctcgg taatgatgtc aaacttacga
     8041 tcgaagggta ttataaaaaa cttgatcatc ttatcgtaaa gccggatcgt acatccaatc
     8101 tgcgtaccaa tcagggcgaa ggttgggcac gcggggtgga catcggtctt gtcaaacgtt
     8161 ttgcacaacg atggtacggc cagatcaact actcacgtat ggcaagccgt cgcaatgacc
     8221 acaacggtga aggatggtac aattcggatt tttctcagcc caacattttc aatgttttat
     8281 tcgggtacga atttaacaaa gaatggtcat tttcgacgaa atggaaatac gcgacgggtc
     8341 gcccgaagga cacgtatgtg attcacgaaa atgtatttaa tgatacggat catatgcgtt
     8401 acagcaaaga gatcattcgt aacaacgggg atcgtctgga tgattttcac acatggaatg
     8461 ttcgtgtgga ttatcggaag caaatcggac gttttgccgt ggtaagcttc gtggatattc
     8521 tcaatatgta cggccatctc aatgttaacg aggagttttt tcagtatcaa aacggtaagt
     8581 tggacaaacg cggatttcgt gtcgctccga cgatgggcgt aaaaatagaa atgtagtttt
     8641 atattttata tgttataacg aataaagatt gcctcaaaaa agtttagatg gatctattca
     8701 tcattggacc cgaaaatttg atgaattgtt tgatgagtag atgtgtgtgt aacggattga
     8761 ggcaatcttt tttttaaatt acttcttatt cgctttctta aaacgtgtaa tcaatttttt
     8821 tatctccgca tcttccgtga tattttcact caatgtgtaa aaccggttag tgtggtttat
     8881 cattttaccg gatttcaaat acaatgtaag ctttccgact ttagtgccat tgccaccggc
     8941 ttgtacgatg tatctatttt tgtccacgat aggttcttcg atttcaacct gatcgtgtgc
     9001 accgacgatc atatcaatat ccggaaagtc cgataaaatc aacttgtctt cttctgaacc
     9061 ggcatgagac agaaccataa gaatatccac tcttgagcga atatctttta ttactgcatt
     9121 caaacgttct ctttcattga cagagtgtac tccgggttgc ttgtttttcg gaaaatatcg
     9181 aaacgcggaa aatggtgtat aaccgatgat tccgatctta tagttctctc tagttacgat
     9241 ctgataaggt tgggcaatgg gtcgggtatc atgccaaagt gtagatgaag tgaatggtaa
     9301 cttagatcgg gagacgatac gctcaaaaaa atcgtatcca ttggcaaact cattatcacc
     9361 cggatttaca gcatcatagg aaagcaattc gtataatcgt atcgtcatag aatcctgctg
     9421 aggagaaaaa ccaaattgat ttaaaaaatc tccggcatct acaaaaataa ctgctctctc
     9481 gtttttcctt atgttgtcga atataaactt tcttcgtgca agcccaccca aaggttcctc
     9541 tccgcaatcg catgcttcaa tatatccgtt ggtattattg gtataaacaa aagaaacaga
     9601 atcctgagcc actgcgctat aagataatag caagggtgcg atgatataaa tcaggataaa
     9661 aaaaaccatg tcgcaatagc gcgacggact gatacaagtg tattggtaca tttttgcgac
     9721 gcttaatata atgttacaat tgaaatctat aatttataac acttaaaagt aaatcatttt
     9781 ttggcataag acttgataca tctgataagt gttatcggtt cttcacattt taaaaatcac
     9841 atagttacta aaaaaaatcg gttctctttt ttataataaa ctaaatgtag gagttacaca
     9901 tgaaaaaatt agttgttctc gctttgttag ttatgctagc cacagcggtt caaaccaatg
     9961 cacaaatgaa agccggaagt atgcgtcccg cgttggagtt gaattttgga ggtcttggtc
    10021 ttggttatgg tgccagcttc gaatatggta tcacagacaa aatcagtgca cgcgccagcg
    10081 caaatatttc aagttacaaa accggcggtg atgaatggtc attgatcccc attgatgcgg
    10141 ttggtgttta taatgttgat gatagtaagt atgccatgct gggcgttacg tggacaactt
    10201 tctcagttga tccgagcatt ggaaaaaagg aaagttcttc gtcgttcggc gtagttgctg
    10261 gtggaggtta tcgctataaa ctcaatgata aaatttcagt caacggagaa ggaaagtacc
    10321 gtgtgatcac acttgaatca ggtgcttata aactttctgt agcatggtac actgtcggcg
    10381 caggcttctc ttacgctttg aattaactta tccagacttc aggtattcca agcctcgctg
    10441 actgttggca gcggggcttt ttttgtttgt ttaatttata tgatactcat accttctcca
    10501 gtcgtttttc gtagtaaata tcttattaat ctttattagg gagggtctat gcgtgtcaat
    10561 ttactagcac tttttctgtt tatgtatgta ggagttacta gtgttttttc gcaggaggaa
    10621 gaagaacata caaagcaaaa aatggctttc atttccggtt cttttagcta ccaatcatcc
    10681 acaaacgcca atgaagaatt gacgatcttt ttggaagatg caaaaatcaa aacgtttagt
    10741 gtacttccta aacttggtct atttatcaat acaaatacgg ctataggtgc aggtgtgggt
    10801 tatacgtacg aaaaattgga agccagtgat tacgatggtg agcttacttc aacaacaccg
    10861 cttttttcga tagagccgtt tatgcgcagt tacagacctt tgactgatga tgcttccatt
    10921 tttgtagatg catcagcgtt ttaccggttt ggtacacaaa cgttcgatta ttatgacgag
    10981 tatgatgagg ataattattc ggaaaaaact gaaaaaacgt cgtatggtgt tgcgataagc
    11041 cctgggatca cttaccatct aactgaaaac attgcaatgg agttggtggt aggaaaattg
    11101 cattacacaa aaactaaaaa taaacctaaa ggcgcaccgg atgatgttgc atttaaaagc
    11161 tcttcgtatg ggctcaaatt agacttagat caagcctcac tcggtgtcgt aatttatttg
    11221 acacgttaac caaaaattgc atggcattac agaaactgta gtttgtccga ttataatcag
    11281 aagtagtgaa cttaatgcgt aattgagggg tagccttcag agatttgttt tttaggaaag
    11341 aaagcttgga tggaaataga cgtagcgcaa aacaaaatat tttttacttg ctttcgccgt
    11401 taaaaaaact aaatttagcg cgtattgtca gaaagctgat aatagttatt catgcgttct
    11461 tttaaagttt atttttacaa tagtttataa ataaaacaaa accgaaggat acctcatgat
    11521 caccgtccaa gtcggcgaaa acgaaccttt tgataaagcg ctcaagcgtt ttaagcgtaa
    11581 atgtttacaa tccggcctta tgcgcacatt gcgcgataca gcgcactatg tgaagccgag
    11641 cgaacgccgc aaagccgatc gcatgcgcgc tatccgtaaa caaaaacaga ttcaggccga
    11701 agaaggcaac tagtctgttt gcgaagtagt tttcaaaaac catccctttt cgggatggtt
    11761 tttctttttt atatgacgaa aggaatggtc atgaaaaaaa tgatgttatt gtttctagcg
    11821 gcgagcctta ccgtttcggc tcaaatcaca cgtcaggtac tgaagcctgt caaagtggat
    11881 atttttaaaa acgggaacgg tttttttgtc gccgaaggaa acctcagtat gagtaacagt
    11941 gaagcgcaaa tcacagatgt gccccggacc gcgtttggaa cgctgtgggt aggcgctatg
    12001 gacaagaaca ttcaaatatc gggtttacgt gcgatcgaag agaaatttgc taatcgccga
    12061 gatccggctt cattgtatga aatattaatc tccaatatcg gcaaacaggt gatatttcgt
    12121 caaagcgggg aaaaggcaat tgaatacaca gggattttag agcgtgtcat aggcgaagta
    12181 tcgcaaccgg ttattgtatt gcgtacgacc aaagggacga tgatattaaa tacgtggtat
    12241 gcgatgcatg ccgagtttcc ggagtctttt cagaatacgt tttccgatac gaccaaagcg
    12301 atggcgctgc gcataaagac caatagtgca gcaaatgccg cacgatttca aatggtctat
    12361 tttcaaaacc agatcgggtg gacgcccagt tacaagatag atctcgttga tgataaaaac
    12421 gcgcaaatca tcttatccgg cacggtagtc aatgatatcc aggatattga aaaagccgat
    12481 gttaatctgg tcgtaggtta tccgcatttt gaatttgcca acacattatc accgcttacc
    12541 acaccgatgc cgatcagtga tttcctgtcc gcgttaggat atgatgcacg tcgccctgat
    12601 tttgataatt atggtaatcg gatgatggcc gcgcaagttg ctcgttatga gatgagcgca
    12661 ggacaagccg atgcgggcaa ttttacacct gtcggaggta cgactgtcga agaccttttc
    12721 ttttataacc tacctgattt ttcattgaaa aaaaatgagc gcgcgcagat caatattttc
    12781 tcggctacgg tgccttatca acatgtctat gaagtaacgc ttcctaatgc atttaatata
    12841 caagaggggt atctctacga taaaaccccg gcgcgcaaag agccttacga agtgtggcac
    12901 tccattcggt tagaaaataa aacggctatg ccgtggacga ccggtgccgc gctgaccatg
    12961 caaaacggca aagctttggg ccaggatatt ttgcagtacg caccggccaa atcaacaacc
    13021 cacgtcaaaa taacgacctc gcccgatatc tatgttacgg aagaggaacg tgaaacagag
    13081 cgcaaagaag atcagaaaaa gaaggatggg tattcgtatg atctcgttac catatccggc
    13141 gagatcaatc tgaacaacta caaagataaa gacgtcaaac tcaaaataac acgccccata
    13201 accggtaaac tgcgcgaagc aacggcggaa cccaaaagta aagtcaacgt caagtcagga
    13261 agtgcgatca atcccgaaac aacattgacg tgggaggtta atctgaaaaa aggcgacgcc
    13321 cgaaagatca cgtatactta cgaagtgtat ctcagacgat gatttaaaaa atcagttaac
    13381 ggtaaagtcc gtcgcgcgga gcaatcgccc ttgtgcgtcc acaatttcaa ccttccagct
    13441 tcctttgcgt ccgctgacgg tacgaaatgc ataagtgcgc atggattgtg atcggatgtc
    13501 caagggcact tcaccgaggg agtttccatt gtgaatccat cgcactttga cggtttttcc
    13561 ctggcctccg ttgacgcgca gccagcaata aattttttct gtagtcgagg aagagaaagt
    13621 atctgccaca ccggtaattt ccatattcgc tacgccggtt ccgattcgca aagattccac
    13681 cagcataccg tcaatcgtat tgccgccggc aggcgttgat tgggtttcag atggtgctgc
    13741 atttttatta gtagttgcat tgctgttatt ggcttttaca gacgtattat tgcctgtatt
    13801 cgaaactgca tttgtaaccg gggcaacatt ggtacttgtc acggtttttg gtttaggggt
    13861 cggtttgtct tcgcctaatt tggagttagc gcgtgtgata ccatagacac tgcgcaaatc
    13921 gtaaggcagg taacgtgcgg gttgttgccg gttttcgccg ccgtaactca gcatcaaagc
    13981 cagtttgttt tctttatcta tccattctac aaaaatagca ctgtgctcaa tatccccgta
    14041 agaatgattg atgtaataca accagtctcc gtttttgatt ttttcaatat caacaaaagg
    14101 accggctttg accgacttga aaacagtttc gcgttttccc cacacgtaac ccgcacgatc
    14161 aaaaaccgca ttggcataat cccaacaact gcccggtagt atttcggcat tggtcaatgt
    14221 catcgttcgg ccggtctcta atattttttt tccatccgct gatgcagttt cttccgcttg
    14281 ggtcagcatc gccgtataat cgcccgtgtc gtaataatcc tgtgaacaga tggagagcga
    14341 aatacccgtg aaaacaaaaa gataggtaat aattttaagc ataccgctat tcctgcgttt
    14401 aaggtgtatg aaatgaaatt taaaaaatga gaaaatggtg aaccgtaaaa taatacagac
    14461 aggaagatat aagtctgtct ttaggacgta cgtaagatag gacgaatcgg cgtgattcgc
    14521 aatttacaat ttagagatat gtacaggctt actcaaatcg tcggtagccg agtgaatgcc
    14581 taattaaacg tgtctttcac tctttcggac ctcatcaaat ataaagtcca tacgatgaac
    14641 atcatcgcgc ctattacaaa ttcggccaaa cgtaccgatg aggcattatg atttgtatct
    14701 gaactcaacc actgtgctga tgtaccgacc agaccgacga aaaccagatt gaccagtaaa
    14761 agcatcatga tcattatcgg ggtgcgacga tccattcgga aaaaataaat cggcaccata
    14821 atcgcataaa caagatagca gagcgtaacg gtgacttctg cgtacgcaag gtagttggat
    14881 gccaagacat tcgcagcggg taatgacact tgtttgagtg cgatcaatat acgaatcgga
    14941 ttaatcagca gaaataaacc taataaccat aaccatcctc cgatacgatg cggatatttg
    15001 atagtatgct ccataagaca cctccttcac aatcaaagta cagtgtagcc tttggcggag
    15061 caatgacgga ggtcagtagc ggcgtatttt ttatcgacat gcgtatggtt tcatttatag
    15121 aatggattat aatttttgat tccgtaaaaa tttccattat cttatccgta tgcgtaagac
    15181 gtggataatt ataactatag cgattttagc gtgttcgccg gaaagtggat cgccgcccaa
    15241 tgaatcgcca ttggtaacgc gtatactcgc acctgccgtc gtagggttga cagatacagt
    15301 aaaaatttat acctatgatg ccgatgagag cacgctacag attgaagcta aaatctttga
    15361 tggtgacggc caagtgctat cggatgtgtg gaaaacgaat ttgcatgacg atggtcttgc
    15421 cggtgatatg aaggccagtg aaggcaacta tatcggagtg atcaaatcgc aaactctttt
    15481 ggatcacaac agcggttttt ttgaattgca agtcatcgtg acagaagcga agagcggagc
    15541 gacggacaag cagtcggtgt ttatagaaca gaatccaaat cgcacgcatc cgcctgaagt
    15601 tatcttagtg acggctcccg atacggtaaa cccaagtctg gtgagtgaat ttttaataac
    15661 cgttacggtt tccgatccgg acggtttggg tgatatagca tccgtgacgc gtacgacgcc
    15721 aagtaatttg gttttagcct tacgtgatga tggtgtgaac ggagatgcga cggcacatga
    15781 tggtgtttac tcagaaaggg tttcggtcaa tccttcaccg ccagcgggga gttatacatt
    15841 tacattcaaa gcgaccgatc ggatcggtct tcaaagcgcc gcgatcccaa aaacgatagt
    15901 cattatttca aattaaaatc gcagcctctg ccgtatagtt tatcaaaacg tatctaaaat
    15961 ccacatcctt acatcaaagc gttacatctt caaaacaact tcttccgtgc tggcaagttt
    16021 ccggcgcgac gtaggagcca tccggcccgg aagtcaacat cgttcgcaaa caccagtact
    16081 gcgctgtagc tgaagcatcc gttcgccatg cgtcatgatg atccggttcc gtaacgtaag
    16141 cggttttagt gcgaatatgt ttacaatctg atcgtgcgtt cattttgatt aaaaattaag
    16201 ttcctgattt tttttgttaa cggcgcgctg taaggtgata tgaccaagta atgcgcaggg
    16261 caggtaggtg ataaaattgg cgacccaata ttgaataggg tgactgatca tgagcatacc
    16321 gatgacaccg aaaaccacat aaaatgcagc gacaatgatc gcattacggt aaccgttttc
    16381 cggtgcaatc caggacgcta cgagaccgcc cataaaagaa cccagcgcca tacccgccac
    16441 cgcaaaaaga aatgcgccta aggaaagtgt tcgcaagtgc gcttccagcg tttcaacgct
    16501 gttaggattg aaatcggcgg ggggaggata tatcgtggca ccgacgtttt gaacgatagt
    16561 caccattacg atcactgtga tgataccgcc gataacggaa aaaatatttt tagccataaa
    16621 acctttcgtc gttgtttgat gtaaaaacta aaacagttta tcgcaaagga tcgataccga
    16681 ccgcaaaaca caaagcgcgt cggatcacgg tttcaaatat ggaaattttg tatgcatttt
    16741 tagatagcgg cgtcgcgttg gataaagctt ttttcgcggc gcacgcggcg gactctttgg
    16801 tgattgtctg accgatgaga aggttttcag catcggtgca acgccagggt atcggagccg
    16861 cggcgcccag cacgatgcgc gccgatcggc agatgttgtt ttcgatagcg agtgcaacgg
    16921 caatttccgc gatcggccaa tcaaacgatt gtttttcgtt ttgccgaaga taaaaactga
    16981 tcgtgccgtt cggcggttcg ggtatgtata tttccgtgat gacttcaccg ggttggagtg
    17041 cgttctcttt tgtaatatcg ttcgccgggg taatgaaaaa ttgatccata gccagttcgc
    17101 gttcaccttt ggacgatttt attttcaggc gcgcatctaa cgctgtcaaa gccacggccg
    17161 gcgacgaagg atgaacgata acacagccgt cggtatttcc aaaaatcgca tgatacgcgt
    17221 tttcgccgtt catcgcgtaa cagatttttc cgcctttgcg tgtacaatca aattcgtgac
    17281 ttctaaagta ccagcagcgt ggcctttggc agagattgcc tccgatcgta gcggcatggc
    17341 ggatttgcgg tgtcgccaaa cttgcacaag cctgagccaa aacgcgaaac gggccggcgt
    17401 aaatattatt tacattggaa atatccgcta tcgtagcgcc ggctccgatg gatagatcat
    17461 catccagaga aattttttgt aacgctgtga ttggacgcag actgactaag cgcgaaggcg
    17521 ctgtcaatcc ttctttcatc agatcaatga tgtccacgcc gccggctttt atcgcggtgt
    17581 ccggttcttg aagtacgggc aaaagtttgt ctaaacttgc actctgtagg tattcaaatt
    17641 tattcatatg tacaaacctt ttggtcttgc tgaagtgtca gatgtttggt tacgactgaa
    17701 gcgcttccag tacatgggcg gcatgcatag ggagtttgcg aatgcgttta cccgttgcgt
    17761 gaaaaaaggc attggctata gccgccgccg tcggtatcgt agcaggttcg ccgatcccca
    17821 tagcgcccgt agcggaagcg ccggtgtaga catccagtat ttccgtttca atgaccggta
    17881 tatctttggc tccggcaatt ttgtattgat caaaattggc gttgaccata cgaccggtat
    17941 acgcatctaa aattctgttt tcaaataaag cataactcaa tccctgtatc acaccgccgc
    18001 gaatctgact ctccagtgtc agccgattga tcacacgacc gcaatcgtgc accgcgacga
    18061 tacgattaac cttgataatg ccggtttcgg tatccacttc gatatctgca aattgtacac
    18121 cggctatgta cggcgggctg agtgtaaggt aatcgcgcgc acgttctccg ataaccgaaa
    18181 atttatcacc gctgatttta gaggcaactt gtttccatgc gagacttttt gacgtatcgc
    18241 gtcgggaaaa aatgcggccg tcggcaacgg acaaatcatc cgacggtaca cccagcatag
    18301 gtgcggcgat ttcaaacatt ttgagtttag caagataagc ggcatgacgt gctgccgggg
    18361 taatacccgc cgtagtctga ctaccgccgc tggcggggcc gaggccatac tgcgtatcgc
    18421 cgattttgat gacgatgtcc ttcgtttgca atcccagttc ttccgcgacg atcatcgcca
    18481 aagcggtgcg gataccgcca ccgatatctt gaacgccgtt ggtcaaatcg acggaaccgt
    18541 cgctatgcac ttcgatggta gcttgaaatc cggtaccgta aatgtaatac catacggcgt
    18601 tggccattcc gaaaccgcgc ttgatcgggc ctttgtcgcc ggcgggttgc gcggcgcgcc
    18661 ggtcccaacc gaaacgtttc gcgccaatgt cgtattcagc caatcgaaca ctatcggttt
    18721 tagaattgat tcgtcgtagg gcgagcgggt ccatattgag tttgtaggcc aattcatcta
    18781 cggattgctc cagtgcaaat gttccctgcg gatgacccgg cgctctaaat gcggcgcctg
    18841 caccggcatg ggtaaagaca tcgtactgct cggagtattt atgaggaacg gcaaaataca
    18901 aattcatcgc cggaccgtcg catccggcgc ctgtaccaac gccggccgtc ccgtgcgcaa
    18961 tcaatttgat ggcttggatt tcacctcgcg cgttagcgcc gacacgaagt ttttgcactg
    19021 cgctggggcg attgcctgca gcgatatgtt cttcctttcg gtccagcata aggcgaacag
    19081 gtgcaccggc tattttggcc aatttggccg cagcgatgcc gtatactcct gcaccgaact
    19141 ttgcaccaaa tccaccgccc atgtattccg taatcacgcg tacatgtgtt ttgggtaact
    19201 gaaagacagt ggctaattca tcccgaacac tgaatgtgcc ttgtgtcgag gcccacaccg
    19261 taagtttgtc cgagttttcc cagaacgcca cgacaccatg cgtttccatt gccgaatggg
    19321 tttgcacttg tgtttcataa acagcatcta cgattacggc gctggatgat aacgcggcat
    19381 ccacagaagc ggcattgcct ttgacggcgg ggccgcgcaa gttgcctttt tgcgtaacgg
    19441 atccgatgga tgtcggttca tcgccttccg atttttgttc ttcaacagct gtgtcaaaaa
    19501 tatgggaagc gcccggtttc atcgcatcct gaatcgtggt aataacccca cgtttttcgt
    19561 attgaatttc gatcagtttt agcgcttcgt tggcggcgtg tgttgacgtc gcggctacgg
    19621 cacaaatttc ctgaccggca aatcgtacga attgtggtat cgtgtcttgt atacgtatca
    19681 ccgcgcgcac gccgggatat ttttcggtgc gggaggtgtc tatcttgcga atgacggctg
    19741 aagcgtgtgg tgagcgcaga atccgagcat gtaacatgtc cggcaattga atgtcgaagg
    19801 tataccgtgc agaacctgtt actttggcct gaccgtccag acgagtttgg tttttgctga
    19861 ccaattgtaa acgatcatcc gcatcccatg gcttcgggac gttatcggga agggaaacgt
    19921 ttttttccgt ccatactccg gaatgaccgg ttttgagttt gatattttta ggcataaaaa
    19981 ctactcaatt ttgttatttc atattttttg cagccgactg tacggcttca aaaacgtgat
    20041 tgtatgtacc gcatcggcaa aggtttccgc tgaccgcatc ttttacgtcc tcgatggacg
    20101 ggtttttatt atcgcgaagt aaagctgcgc aactcatcac catacccggt gtacaaaatc
    20161 cgcattgcaa agcgtcatgt tcgataaaag cttgctgtag cgggtgcagc gacataccat
    20221 ccgaaagccc ttcgatcgtc gtaatctcgg acccgatcgc atccacagcc agagtgaggc
    20281 atgcgtttat ggttttatca ttcatccata ctgtacacgc accgcaggct ccgcgttcac
    20341 atcccgtttt ggtgcctgta agatggagat gatcacgcag cacttcggcc aatgtccagc
    20401 gcggttcgac acgcaaggat tgttttttgc cgttgacgga taatgtgatg tccgttgcac
    20461 ccggacccag tgtttggata tcctgcgtcg gattggcaca ggaagcttgc agtgcgccga
    20521 tgataccgcc gttggcggcg acagcgccca gagatgagag gccgaaatct ttgaggaaag
    20581 cgcggcgtga tggatccggc aattcaccgg catcgtcttg cggttctttc ataagctgct
    20641 cttcaaagtt ggtatggtgt tcgatatgtt aagttttatt aacggaaaaa tcagggttaa
    20701 tgtttcagaa tcgcaaagtt gttgtgatcc atcaacccaa aacaaaatcc acctcataca
    20761 ttgcataatg cgcttcattc ataccgcaac gatgatatac attcatggct ttgcgattac
    20821 ttttgtccac atatagtcgt atgccgcaca cgtctttttt agtgcgcgcc tgctcaagta
    20881 catactggta caatttgcga tatataccca tgccgcgaaa ttccgggtca acatacacgc
    20941 tttgaatcca ccagaaaacc ccgtttcgcc aatcgctcca ttcgtacgtg atcatgagtt
    21001 gtccgacgac cgtgccatcg ggccgttcgg cgagtaaata aaaaccaaat tgcggctgac
    21061 gaaacagatt ggttacgcca tcgcgtaaaa tatggcgcgg taattcttta ttttcagttt
    21121 ctttggacat agcggcatta ttgtgaacaa ggatatccgt atcggaaacc gttgctaaac
    21181 gtatatggat ggccataaag ttaatgcagt tataatgaag ggattatagc gctataatac
    21241 gcttttccta atttggttac aaatagagaa taaccggtgc actttgcaga cgcttattgc
    21301 cttagtcggt atcggcactt atatttgcag atgacgatga aaatcagtgt gatcataccc
    21361 gcctacaacg aagcctcgat tatcggtgaa acgctttcgg cattgatgaa agccgaccgt
    21421 ccatcgaacc cgatagagtt tatcgttgtc aataacgcca gcacggatcg tacggcggag
    21481 attgcctctt cgtttcccga catccgtgtc gttacggaat cggaaaaagg tcttaccaaa
    21541 gcgcgtcagt gcggcaacgc ccatgcgcaa ggcgatatcc tgatctatat tgatgccgat
    21601 acgcgcattc ctaagcacct tttacgaacg atcgaagaaa aatttatagc cgatcccaaa
    21661 cttgttgcga tgtcgggccc ttataagtat cacgactgga cgtggtacgg acggttgatc
    21721 ttgtggcttt atcactggac tttggttccg atgaaccaat tggtgatcaa tcggatactc
    21781 aataagggta gtgtttttta cggcggtaat tttgccgtga gaaaaaaagc gctggataaa
    21841 ataggcggct ttgataccgg attagaattt tggagtgaag atacgcagat cggtcgccgt
    21901 atgagccagg aaggccgtgt tcgttttttt caccgggcgt atgtttatac atcggcacgt
    21961 cgttattatg cagagggatt tcttcgcgtg ctcatgcggt atatcatgaa ttatttttgg
    22021 gatattattt ttcatcgccc tttcaccaaa ggttatcagg acgtacgttc taccccgccg
    22081 aagcaaacga cttaaaatct tccaacgttt acggtgtaat ttgttttaca aaaaccgatc
    22141 gcgctttttc agccggatta gtgtcgggat agtccagaac cgccgtattg atgtatatct
    22201 ggttggttat gattttttgg atttctttac ctaccgcaaa agcgtatagc gctgtatcgg
    22261 catccgcttc gatggggata ctccactcga tgcgatcccc tcgtttactc gcgatgtagt
    22321 aaccgtcttt attgatatac acatggcatg cggtagccaa ggtaggatgc attagacgcg
    22381 ccgtgccgcg gaggaaccat ctttctttgc gggtgatggc gatgtctttg cgcagcaaaa
    22441 gaacgggcga cgggcttaga gcctgtgtga gcgataggac gcttttgaca gtgtcctgaa
    22501 aaaacaaagc atattctttg gtttgttcgt tactggcgcg ggctagcgta agaatcatac
    22561 cgggttcata ggggatttcg atatgtatag gcacactgtt gccgatcgcg ttgacaaact
    22621 gaacgcgcgg gccggccgca acaagcatgc tgcgatccga ttgaaattgc agatccaaca
    22681 cgagaaaatc atcgctgcgc ttttcgataa ataaagaatc catcatcaga tcgcgttcgc
    22741 aaaaaacctg cgtaccgtaa tgacgtatcg tataacgcgc gagacgcgtt ttggcgccgc
    22801 ggatcagcgt atctttgatc acactgtctt tgtatgaagc aataccgata ccgatatgcg
    22861 taaattccgc tttcaatata ttagcgcgat ggccgggact attcatccac tgcttcatca
    22921 gttgatcggc gatgctgcga tatccgccat ccacgctatg caaagcaata ttttcgccga
    22981 gcgatgcatc cgatgtcccc gtgcgatata cgcggtcgga tacggttttt agtgaagcat
    23041 ttggggaagt atgagaaaaa taaccctgtg tcattatttc acgggaatgt tgccgggccg
    23101 ctgagcgtaa ggctttatcc cacacgagag gcggaagttt agctttggtg cgttcaatat
    23161 ttacctgatt ccaaacttcc atttcggcta aagagtcgcc cggtagcgcc gaaaattcgt
    23221 catgcaggta ttggatctgt tgtccccaaa tcgctgtcgt caggcataat gaaacaaata
    23281 aaaataactt tttgatcatg acgaattatg ctcctgttga ggcgaggcgt aaagcctgtt
    23341 tgatcatatc ctgtagagca aacggcttat cacccgcttc acgtaataag cgagcgatgg
    23401 ctttttcggc aacgggtttg gagtagccga gagagatcag tgccattaag gcttcatcgg
    23461 cgacctgcag attggccgat gacgtgctaa gcttagcggt tgatgcgacg tcgcccgttt
    23521 tggtgatttt gtctttgagt tcgacgacga tacgttgtgc ggtttttttg ccgatacccg
    23581 gaatggccgt cagcaattgt acgtcttcgt tgaggacagc ggctttaaaa tcacggatcg
    23641 cgatgccgga catcatactg atgccggact tggggccgat gccggagacg gaaatcaaaa
    23701 gtaaaaaaaa cgagcgctct tcagcgctta caaagccgaa taactgcatg atgtcttcgc
    23761 gcacatgcat atacgtcagt agcttgcaag ccgcgcctac ggccgggatt ttttcgtagg
    23821 tgctcacggg tataaaaagt tcgtagccga taccctgtac gtcaataacg gcgcgtgtcg
    23881 gttctttttc ggccactagg ccttggacaa atgtaatcat gattaaagat atcctgattt
    23941 ggctaaactg gtatagtgac gtcccgcgat gatgatatga tcgcgaacgg ggatactcat
    24001 aataatgccg gcttctttga gctgttcggt gaccgcgata tcctcccggc tcggttccgg
    24061 atttccgctg ggatgattat gtacgaggat aatacttgcc gccatatatt cgatcgccgc
    24121 tcgaaacact tcgcggggat gtaccaaact ggcattgagt gtgccttcgg agatcgtgca
    24181 atcacgtatg atctgattgg cgctgtccag caataccacc ataaaaattt cccgattaag
    24241 atcgcgtaac cgtggcatat agtgtttgaa taaatcctcc ggcgcacgta cacggatttt
    24301 ttttgcatcg ctttgagcct cgatgcgacg ggataattcg aaagccgcgg ccaatttgat
    24361 ggacttcact ttaccgatgc ctttaaaacg gcgcaattcg tgcgtcgaac accgcgccag
    24421 tgcggcaagg cctgagaatt ccttttcgag cgcacgcgcc aaatcaacgg ctgtaatatt
    24481 acgcgtaccg ctgccgatga gtatagctat taattcggcg gaactcaatc gtgcggcacc
    24541 gagttgttct aatttttccc gcggtcggtc ttgcgaagac cagtctttga tcttggcatg
    24601 atagagcgaa ggttcggcta ttttttttgg tttacccacg atggtttcga ttgcctatac
    24661 ttgcgctgcg ccgcaattgt cgcaaaaatt ggtttccgaa cgaagttgtt ttccgcacgt
    24721 cgtacaatat ttaaccgcgc cggtaaaggt aggcgcatca ttggcggctg ccggatttac
    24781 gcaatcttca taggccactg cggcaaatgt aaactgcaag ccgattgaaa aaacgatcgg
    24841 tatatagcac gcatataaac cgacttgtgc gatgatttgt gcaaccaaag caaagagtgt
    24901 aaacataagc caatcttttt tggctatgga aaaacttttt tgaatagctt ccataggccc
    24961 caggccttcg tatacagcga tgggtatggc caaataaaaa aaaccgccga cgataaaagc
    25021 cggaaaaacg caaaaaacga atccgatgca cgcggcgaaa acatatacaa gacaaataac
    25081 taatactgtg aaaaaatggc cgccaccact aaaggcatcc gatacggaga tggactcccc
    25141 ttttaattgt ttacgcgcgg tgaggtacat gcccagtaaa aggaaaagca taaaaaggcc
    25201 gattcccaaa acgagaaggc cgaacattac catacccggc aaagccacat ccggatccat
    25261 gtgttttccg agaaagggga gtgcgaaaat gctggcatac aacatgatga gtgcgacgac
    25321 tagcgggccg aaataaagag atcccattag agcccacgta ccccagtctt tgcgcagcaa
    25381 atcaaatccg cgcgaaatcc aatcgccaaa ttttaccgtg tttcccatac atcctttctc
    25441 agttatactt gtgatgcgcc gcacttatcg caaaacgatg cgcggacatc tatggaagct
    25501 ccgcatcgaa tacaggactt tgagtataat gcgtccacgc gagatatcgg ttcttccgtt
    25561 ttgatttcaa aacagtctct gtatgctgcg gctgtcgtgg tcactatcaa aggggccgta
    25621 ataaaaatac cgattccgca caatagaatg ccaagaaacg ccagcacctg cgttacgatc
    25681 caaaggacgg tgtacatgac ccaatcgctt ttggtcgttt caaaactttt tttcaaggct
    25741 tccaatggcg gaagattttc gcgaacaacc atcggtacga catagtacaa aagtccttgt
    25801 gcgatcaaac cgggaaatag acacaacatg ctgccgatca tgctaaacaa actgactgaa
    25861 agcattgcga ctaataccga tacataaata tccacgccgg agaaagcatc gctaacggaa
    25921 atggattcgc cctgaatttg gcgaaatgcc gcccgaaaag cgccgcccat aaaaaaagcg
    25981 ataataaata ccatggcaaa ttgcgtgagc atattgacgg caaacgacga ttccatggag
    26041 cgaagaaaat aatccgtcag gctttcgtta gggccgatct gtagcgaacg gccgatcagc
    26101 atttggatca tgatatagac aaatccggcc ggaataaaag tcaatgcact gataccggtc
    26161 catgccatcc attcttgacg cagcatcgtc caaccttcgc tcagccattt gcccaaacgt
    26221 acttgataat tcggattcat agttcctcaa aaaaataaaa agcgggttgc gattaacaac
    26281 ccgcataata atcaattcct actttttaca aaagcaaaaa accaaactat cagtgcgatg
    26341 aagtgccgcc ggcaccgggc atacagcaag acccggctgg cgcttgttcg atcacttggg
    26401 cgtggggttt ggtctctttg ccttccttat gcggtgcgtg cggatgccat ccgaccatat
    26461 acagcattat aaaaaagccg atgatatagg ctacggcaac gtgccatccg tctttgatcc
    26521 aaaatactac ggatttgcct ttggggaaca tattggtcaa tgcgacgccg gccgacgatc
    26581 cgaaccaaat catggaaccg ccgaaaccta ctgtaaatgc caaaataccc caatcgtagc
    26641 cgccttgtgt cagagccaat ttggtcagcg gaatattatc aaatacagca gaaacaaaac
    26701 caaggctgaa ggctgtttcc caggaagccg gaggcagttc ctcgacgggc atcatcgaag
    26761 cgcacatcac caaggataat aggaaaatac tacctttcaa agcaccgggc aattcatgcc
    26821 atgcagtttt gcgaaatgtc gcgccaatta aaatcgcgat ccatacgccg acggcgggga
    26881 aatccaacat aaagttggtt gtaatcgcac cgacgaggat aaggaagaca actaaaattt
    26941 tcccccagtc aatggcgata tcttcggcat gggccgcgac gataggttga aatttatgtt
    27001 gttgtttggc ggcgataaaa ccgaaaacta aaaacgaagc aatcgaaccg acaaaggcat
    27061 gcgtaacatc caatgcattt acgccgtcta tccacatcat cgttgtcgtc gtatcgccga
    27121 cgacggatcc ggcaccaccg gcattgcttg cagcaacgat cgccgcaaga aatccgatat
    27181 gcacattgcc tttgaaaacg accatagcga tggtgccgcc gatcattgcg gccgcgatat
    27241 tatctaaaaa cgacgaaagt atcatcacga taaataataa aacaaaaccg cccatccaac
    27301 ccgaaggcag atatttggga agaatttcgg ggattttgga ttcttcaaag tgtttggcca
    27361 aaagtgcaaa tccaagcagc aaaccgagca gattggtcaa gatttcccat tcgtgatgaa
    27421 gatgattcat caaattaaaa ttggtaaacc caagtttgaa agttaagatg gaagccaagc
    27481 cgataagcgc gacttgtaaa acgtagtggt gaaaaagcgc cacaccaagc agcgtcaaag
    27541 caaaaagggt aaattcgaaa ggaataccga aaaacataat ttgactgtga ccaagggatt
    27601 caaccgtgga aaacatcggt tgactggaaa acagcgacat catgccgatc acggccaaaa
    27661 caaaaagacc gataataacc ggcgcggtaa aaaaaccgcc tttttgtgtt tcactcatag
    27721 acgctcctcc tgtgtagggt taaacaattt ttcgttcgca aaacggtata ttacaataac
    27781 gatttgtctt tatggtattt ggatttgcga tactcggcgg caaattcaca atatacttcc
    27841 caattgcggc gtatcatcgc caatgtttgt tcatcggctg tttttttgac tttggcaggc
    27901 acacccatga caaccgagtt gggtggaata atctgatttt gcatgacgat cgcacccgcc
    27961 gcgacaatgc agttttcacc gacttcggca ccgttgagta tcgtcgcatg catgccgatg
    28021 atgcagtttt tgcgaatggt tgcgccgtgc acgatggcgc tgtgacctat agaaacattg
    28081 tcttcgataa tggcgggaaa atgatcgtcc acatgcacga cggcgttgtc ctgtatattg
    28141 cagttttttc cgatgatgat ggggtccatg tcgcaacgta aaacggcgcc aaaccagatg
    28201 ccgcaatttt cgccgatttt tacgccgccg atgacagaag cggtattggc gataaaaacg
    28261 ctgtcgtgaa tttcaggcgg tttaagatca gacaaacgaa aaggtttatg gatgggttcc
    28321 acgatgcctc ggtgtacaca atgtttaaac ggtggttggg atgttgattc agcggcggta
    28381 agttctaaaa aaaaagattc aaagtcaaat cgcgagcctt aaccactctc agcttatccg
    28441 ggtatctcgg aagttttctt gcacttatcg gccgcagagc gtataatctg ttatgaatga
    28501 acgtgaagaa atagaagcgc tgatccaaga agcgctgcat gcgcgatcat ttgccgtggc
    28561 gccatattcc aaatttcagg tcggcgcttt ggtggtaatg aagtccggca aacgattcac
    28621 cggatgtaat gtcgaggtga gttcgtacgg tctcacgatc tgtgccgagc gcacggcgat
    28681 ttttaagtcg ctttcggaag gcgaacggga tgtgcatacc gtcgtggttg ttgccgatac
    28741 gaccgggccg tgttttcctt gcggggcgtg ccgtcaggta ctttccgatt ttgcacccaa
    28801 tgcgcggata atttgcagta atattcatgg tgtgtattcc gttttcagca tgaaagaact
    28861 tttgcctcac gcttttgatg caaacgatct ccatccgtaa atttatttaa tgtttaaatt
    28921 tttcaactaa aggtctgttt aaaaatggca gaatggtttg gtgtatttca ggtcgaagat
    28981 ccggtatggc ggcatttgat acaagccggt gtgataattg tcggcgggta tgtattggct
    29041 aagcttttgt atcgtttggt tcatattatt ggctacaaaa tttccgctaa aacagaaacg
    29101 gatctcgacg ataaaatcgt tgccattttt gaaaaatccg tacaacggat cgtcaatacg
    29161 acggcgcttt atttagctat caatcggtta gaacctctat tccaaggtac gtggcttaac
    29221 tacgttgacg gcgctttttt tgtcgtgatg gtcattctcg cgaccatact cctgaccagt
    29281 gtaactaaaa caatcatcga atggtatgtg gctaccatcg cggtgaggac ggagagtccg
    29341 ctggatgaag aaatacttcc gctgatccgt cgtcttctta atatgttact ttattctatc
    29401 gcactgatga tctgtcttga tcattttcat atcgacatca aagcgcttgt cgtgtcgctt
    29461 ggtgtgggtt cgtttgccat tgcgtttgcg gcgcaggaaa cgttgtcgaa tatgatcgcg
    29521 ggctttgtga tcatggtgga tcgcccgttt cgcgtggggg atcgtattcg tattacggcc
    29581 ggcaatatca ccggcgatat cgtcaaagtc ggtcttcgca gcactcagat tcttgatttt
    29641 gattacaata tagtcaccgt gcccaacgca cagattgtca aatcagatat tatcaatttt
    29701 gcgtatccgg atcccgcgat gcggttgcgt atcgaagtgg gcatagccta cggcagcgac
    29761 atcgaaaggg cgaaaaaaat attgctggag gtattgcaaa gcgatgagcg catcctcaaa
    29821 gaaccgttgc ctgaagtggc tgtgacaggt tttggcgatt cggctgtgca actggtggcg
    29881 atcgggcgcg ttcggcatta taaagaacat ttcgagaccg gcgacacggt tcgggaccgg
    29941 atccattcat catttgcccg caacggcatt gagatacctt atccgcatcg ggtgattatt
    30001 gcccgtaccg atcaaaacgc aaaaaacggc tgatttttca gggtatgatt ttataaaaaa
    30061 acgagttttt ttgaattgct taacttgctt ttttaggcgt acctgtctat attcgcgggc
    30121 gtaaccctgc gttcaattat ctgcataatt gtggtaactt tcgatagagt aaaatgaatt
    30181 tcccggagaa aacaatgctc aaagtacgtt taaccggtat tgcggtttgg gccgcagtgc
    30241 ttctgtcttt ttttgcacaa acggcacaaa gccaatccaa tgcacgcggc atgttcgaat
    30301 ttgtgtcccg cgtgggaaat cccgacgacg gcgctacaga cctcgttttg gacggaaaaa
    30361 cgctttttgt tgccaataaa ttcagcggcc ttcagatcat tgatatttcg gatatccgcc
    30421 gcccgattat tcgcagccgt accaaatcgg aaggtcagaa ttttggtatc gccaaaaaag
    30481 acaaaatggt gtttttatcg gataataaag ccggtttggt tatctatgat gttaccagtt
    30541 tgtccaaacc taaactcgtg acatcctttg aaacgcgcgg tgaagcgtgg gacgtccaga
    30601 tcaaagataa ttatgctttt gtagcggccg ggcttgccgg tcttctcgtt attgacatta
    30661 ccgatcccaa agcgcctaaa gaagtcggtt ctttgcgttt tgataaagat tgggactttg
    30721 cgcgtcagct tttgattcgt gaaaaccgct tgtaccttgc tgatcgtaaa gccggcgtac
    30781 atatcatcaa tatcgaaaac cctcttgcgc ccaaagagat caaacgtttt cctacgcagt
    30841 ttgccgaagg tttggatgta tgggataact acatctgtgt ggcggacggg ccgagcggct
    30901 ttgtgatttt ggatatcacc aatcccgaaa aaatcagaca aatcgcagcg caatctttgc
    30961 ccggttatgc taaccgtatc ttcaaagaag ggccgtatgt ttacatcggt gttgacgatg
    31021 cgggtattcg cgcttatgat gtgagcaatc cggccaaacc gttttttgat gcacgttacg
    31081 atacgccggg tcaaacgttc ggtatgatca aatatgatat tttccttgtt gcggccgatt
    31141 tgaatgcggt tctttttctc acgcacaaca agccgccggt tattacgcgt tccggcgata
    31201 aggtcatagc cgaaaaccag acgttgcgtt ttaaaatcaa aggtatggat ccggataaca
    31261 atccgatcgg tttttctgcc aattttattc cacaaggtgc atcgtttgat atcaaagata
    31321 ctgttttttc gtggacacct tctttcgaac aatccggcgt atatgagggg atagtattct
    31381 ccgcgaccga aaagacgcaa tcggctttat tttcgcgtga tacgatcaaa atcactgtaa
    31441 ataatgtcaa tcgcgctccg gcgcttcctt ccacgggaaa ttacacggta gatgaaaata
    31501 aagaattggc ttttacgatc aataagccgt ccgatccgga tatcgaagat acgacaaacc
    31561 tcaaagtcga attgactaat ccgccgaccg gtgcgatatt tgacagtaag acgctgactt
    31621 tcaaatggat tcccgcttac gatcaatccg gtgtatacac attgtctttt gtggtgacag
    31681 acggtggtgg cgcgacggat acaaaacaaa caacgttgac ggttaaaaac atcaatcgcc
    31741 ctcctatgtt tgatgcgctt gcggcatcgt tcacgacgga cgaaaacaag ccggtatcat
    31801 ttacgatcgt cgccagcgat ttggataaag aagacgccgg aaagttggag tggggtggat
    31861 tcaatattcc tcccggcgca acgtttgacc gcagcagcgg tgttttaacg tggacgcctt
    31921 cgtatgacca atcgggcaaa tacgacggga tttattttat cgtgcgtgat gccgaaggat
    31981 tgagcgacac cgtcaataca tcaatcaccg taaaccatgt caatcgtccg cctttctttg
    32041 ctaccgtgga tgcgcaaaag attgatgaag tgaaaccgtt atctttcaaa atagcggcgg
    32101 cggatgcgga tgccgaagac gacggcaagc tgacaatcaa agcgacgacc gttcctcaag
    32161 gtgcaatata taatgaaata acccgcactt tgacatggac accgacctat gatcaatccg
    32221 gcgactacac ggctgtattc aaagtaaccg atccggccgg tgccagcgat gaaatgactg
    32281 tacctgttca agtcgtgaat atcaatcgtg cgccaagtat cgctgcagtt cctgcacaaa
    32341 atgcggatga aaataaagcc tatacattgg tcgtaacgga aggtacggat ccggataaag
    32401 aagacgtagg caagcttact tacaaagctg aaaatctacc gcaaggtgcg actttcgatc
    32461 cggcgacgcg tacgatcgca tggacaccga cgtacgatca atccggcgta tatgaaggcg
    32521 tcaaagtaac cgttaccgat gcacttggac ttacagctac gacgacattt cgattcacgg
    32581 tcaaaaatat caatcgtcct ccggtaatgg aagtgattgc agcgcaaact tcggatgagc
    32641 agaaaccttt gactttccag gtcaaatttt ccgatccgga taaagaagat gcgaatcgtt
    32701 tgacggtagc tgcagccgga atgccgcttg gcgcttcctt tgccggcggt aattttacgt
    32761 ggacaccgac gtttgatcaa gcgggtaact atacggttaa atttacggta actgattttg
    32821 aaaaattatc ggacaccaaa gaagcggtca taacgattaa taacgtcaat cgtccgccgg
    32881 tattggctgc gatccttgcg caagccgcga cagaaaatac tcctttcaca tttaccgttc
    32941 ctgcagcgaa cgacccggat aaagaagatg ccggcaagct cacgtataaa gcagaaggat
    33001 tgccggaagg tgcaaatttt gatgcggcaa cacgtgtcgt caagtggaca ccgacgtttg
    33061 atcaatccgg cgatcacaat atacgcatta ctgttacgga tgtattgggc gtcgcagata
    33121 acaaagaact cgtaatcaaa gttaagaatg taaaccgcac acctgttgta acggatgtgg
    33181 ccgcacaaaa cggcgatgaa aatgcgaaga tggagtttcg cgttgaaacc agtgatccgg
    33241 ataaagaaga tgcggacaaa ctgaaagtag tggcggagca aatgccgcaa ggcgctacgt
    33301 ttgccaataa tacctttagc tggacgccga cgttcgatca agccgggact taccgcgtga
    33361 ctttccgagc aacggatgtg gccggaggat ttgccgtcaa aacagccgtg atcaccatca
    33421 aaaatgtaaa tcgcaatcct gttctcggtg cgatcaataa tcagacggcc gacgaaaata
    33481 aatttatgtc atttacagtg tcggccagcg atcctgataa agaagatcaa attcgcttaa
    33541 atgttacggc ttcggacctt ccgaaaggcg cgacgtatgc ggcgggtaaa ttttcatgga
    33601 tgcccggata tgatcaagcc ggagattata cggtgaagtt tacggctaag gatccgaacg
    33661 gcggtgaaga tgtaaaatcc gtttccatca aagtgaataa tactaatcgc gcaccgaaaa
    33721 tgaatgctgt cgggacaaaa aatggcaaag aaggatctga gatttcattc agtgcatcag
    33781 ccagtgatcc ggataatgat aatctgtcgt tcagcctcgt tggcggccct tcgggtgcaa
    33841 ctatgagcag cgatgggaaa tttacgtgga caccgcaaac cggtcagtcg ggcacattta
    33901 actttacagt caaagtaagt gacggtacgg ccgaagcgtc tgctccggtt accgtaattg
    33961 ttgccaaagc accacaaccg caaggcggcg gtcaataagc tttaaacatg atctgaaaaa
    34021 atcaaagccc tttggtaaaa caaagggctt tgttgtttta tgcaataatc cgtatgtgta
    34081 gatagaaatg atcgaataga aagtattctg atatcaaatt caacagcaag agctacacct
    34141 gaatgtaacg catgtgaata gaatagtcat tgtataacgc ttacggctta taccatactc
    34201 ttacgtttct taataagtgt catactgttg gtacaaataa aaaaacccgc taaagacatt
    34261 agcgggtttt tacgatcaaa caaataaact gtaattccta attaattggc acgactccaa
    34321 tacgaagtgc gcccgatcaa tgaaaaccct atgaatccgc ggattttcaa tttattctgt
    34381 tccatgagct gcatatagca tttataatca gaaccttttt tgggatcata gatcataccg
    34441 ttgctccatt caccttcagc ggagtcatat gtgaagtccc gcaaaatcac caaaccgatg
    34501 attttttgtg agcgacgacc cgaatcgggg tttttgaaat ctactttttc ggcgccaagt
    34561 ttgactaagg gttcgccttc attatcgttt acatcattgg cgttatacgt aggttctttg
    34621 agccatacga ttttgccaaa atactgtgaa ccctgtttgt aaatttcgat ttgtgccgtg
    34681 ccttcttcat tgtaccactt accgatgatg gcatccggat cgcccgcaaa tgcggttgac
    34741 aaaccgacta ccataagtaa accgaggatc ataaaacgct tcatacagcg cctcctttga
    34801 atgatgaatt aagaagaatg atctttctta tatgttcgtg atttacgaaa cggggaatcc
    34861 tcttttgcat gagatgatcg tttggcgaaa ggcatacgcg gtgcgccgcc ggagcgtccg
    34921 tccgtccgtt cgacgacggc tttttttccg ccgatggtta tgcctgtgag ggaacggatc
    34981 acacgatcca cggattcggc atccacgcgg aacacggaat gatcttcatt gataaatact
    35041 tttcccacct gtcgtccttc aaggcgcgcc atattacata tttttccgac gagcgtaccg
    35101 gtttgcagat tgtgctgagc gccgacattg atacgcagtt cggtcatctc tgcacgcggt
    35161 gcatagctgc ggtcacgcat tccgctacgc tgcccgaaat tatccggtac attaagttct
    35221 ttgtcgtctt cgtatttttc caaaaattca cggcaaagca aggttaccaa accgcgcatg
    35281 atttcttcgg tttcatattt tttgattatg ggtttccacc ggtcaaaata acgatcaaac
    35341 ccggctggat ttttcaaagc ctcttcaaat tctgtaatcg tccggtcaat attggcttcc
    35401 atgatttctt gtatgccggg aatcgtggac ttggtaatac gtttacgagt attgccttca
    35461 aggcgacgaa gcttataatt ttccgacgga gttaccaggg taatggcaat tcctgttttg
    35521 ccggcacgtc cggtacgacc gatgcggtga atatagcttt cggattccat cggcagcgaa
    35581 taattgatga cgtgcgtaag atcattgacg tcaatgccac gcgcggcaac gtccgtcgcg
    35641 atcaacaggc gcacgccttt agctttaaat ttacgcatca catgctcgcg ttgggcctga
    35701 ttcatgtcac cgtgcaaagc ctcggcttta aatcctttga gcaccaagcg ctcggcgact
    35761 tcggcgcact gagctttggt ctgacaaaaa acgatcgcgt acatttcttt ggcgtgatca
    35821 ataagccggc ataacgcttc aaacttattg tgttcctgca cggcgtaata aatctcttcg
    35881 gtgttttccg tcgtggccgt gtttttttga attttcactt cacgcggatc ggtcataaat
    35941 cgtttggcaa taccgcgaat ttgtccgctg agcgtagccg aaaaaagcca gatgctgcgt
    36001 tcggtgcccg catgcgtaag aatttcttcg atttcctcca gaaaacccat gtcaagcatt
    36061 tcgtcggctt catcgaggac aaagtgtttg atctgcgaaa gctgtaatgt gccgcgatgc
    36121 aagtgatcta cgacgcgacc gggcgtaccg acgacgatct gcacaccttg gcgcaacgca
    36181 cggagctgta cgccgatatc ttgaccgccg taaatcggaa gaatgcgcag gcgcttatgt
    36241 ttacttaatt tttttaattc ctcgctgatt tgcacggcta attcgcgtgt cggggaaaga
    36301 ataatagcct gtaccgtttt agcggaggga tcaatttttt cgatgagcgg aatgccaaaa
    36361 gccgcggttt taccggtacc ggtttgtgcc tggccgacaa aatcgccatg gtgtgataaa
    36421 agcagaggaa tggcttttga ttgaatttca gacggtgtga caaaacccat ttcttcgatg
    36481 gctttttgca cttcgggact aagagaatac tctttgaatg tggacatact ttccttggga
    36541 aacgctgaca tgagaaatca ataaattatg actatgacgt tttatgaaat atcagcggtc
    36601 ttcataaaaa tcataaaaga caggtttata aaatttgcac gctgtcgcgg cgaacaaaag
    36661 aatcggccac cacatcggtt tgttcatcgg cgtgatacga actccgtacc agcggcccgg
    36721 actccaccac acgaaaaccc atagccatgc ctttttcgcg gaacatggca aaatcatcgg
    36781 gatgaacaaa acgatccacg gggatatgct ctttggtagg ctggagatat tggccgagtg
    36841 tgagtatgtc caaatccact tttcgcagat cgtccatgac ggcaagtact tcatcgtttt
    36901 tttcgccaag accgagcatg agtccgcttt tggtgatcat tccgtttttc ttggagcgat
    36961 caagcacctc caacgtttgt gcatagttgg cctgggggcg tacgacctga tacaaacgcg
    37021 gaacagtttc cgtattgtga ttgagtatat cagggtgcgc atcaatgacg tttttgagat
    37081 tttcaaaatt gcccttaaaa tcagggatca gtacttcgat gcggcatgtg ggaatggctt
    37141 tacgaattgc gcgaatcgtt tcggcaaaaa tcatagaacc gccgtccgcc agttcatccc
    37201 gattgacgga cgtgattacg gcatgacgaa gattcatcgt tcgcacggac tcggcaacgc
    37261 gccggggttc gtctttatcg agccatgtcg gtttacctgt tttgaccgca caaaaaccgc
    37321 aactgcgggt acaggtatcg ccgagtatca taaacgtggc cgtgccgcga ttccaacatt
    37381 cggacagatt agggcaacgg gcttcttcgc agacggtatg cagttttttg gaaccgacaa
    37441 ggtttttgat ttccgaaaat ttttcaccga tcggaatttt gactttgagc cattcggggc
    37501 gcttgagcgg ttctttttcc tgaacttctt cggttttctt tttgatgtag tcaacctgta
    37561 cggaagtcat cggatattga cgggacatag cgaactttct tacttctcag tttcgagacg
    37621 ttatatcaaa tcccgacgcc aaaagcaaag ggaaaatgaa tcattttaat ctcgggtatt
    37681 cctgcattat tcgtcgttgt taactcattt gacgtacacg atatcaccgg cggcgataaa
    37741 cttgatccgc ccttcatttt ctttggtaaa acccacggtt ttatcggttt cgcggtcttt
    37801 ccattcagtc agaacctcta cggtgcgagc gctccacact tcgacgcgga gattgtgcgt
    37861 accctcgggc aattcatcaa aacagcgttg caaatcactt ggaagttcca taagccaaga
    37921 tgaccaatct gactccggca gggcatcacc gcgcgattcg atttcataaa tgcgtttacc
    37981 gttttcgatg acacggtatt cgattacttc cggcattccg cccggcaggc cgcccatgtg
    38041 tacggggcta tatacgcgcc caaaaaaagt gtcgccgtga ttaaattgag tttgcaataa
    38101 gggttgcacc tctgcggttt taccgagacg cgggatttct tctttggaaa aaaccacggc
    38161 gccggcgccc caccattcct gccctttggt cttacggcgt tcatcatccg tttttttgat
    38221 aatatatttg tggaactcac cttgcggctg ctgtgctgtg accattgcca ctcctatgaa
    38281 agtaaatagg atgcaaaaca tgataaaaag tttcatacgg atgctccgtt tttttttatt
    38341 taacgcataa gcagaaagga caaaagaatc aggcagggtt ccagcccatc ggtttgccgc
    38401 caaggatatg caaatgaaga tgaaaaactt cttggccggc gtatttacca ttatttacga
    38461 cgacgcgaaa accattgtct ttgatgccgg ccgcttcggc tactttgcct acggcaagta
    38521 ataattttcc gccgagctcc gaatcatcgc ccgtcatgtc ggataatttt tcgatatgtt
    38581 ttttgggtat caataaaata tgagtcggcg ctttaggatg cagatcatga aacgcgataa
    38641 catcgctgtc ttcatataca ggtttggacg ggatacggtg atcggcgatt ttgcaaaaaa
    38701 tacaatcatt cataaaaaac tcctacgcta tcggtttttg caagtgcggc aattcgtaat
    38761 aattcatcaa gccgttaaaa gagcggcgaa ggaatcccgg aagtatggct ttgtacgtac
    38821 gcaaaagttt attaggattg agaacagatt gaataaattc ttcatatgct ttatcgccca
    38881 gcacttcgat gatgtgtgat ttttcgtccg gatccgtaaa ccgcatcaga atagcgctct
    38941 tttcagcctc gggatgaaac tgtacggact ccatttcatc cgaatagcga ataccagtga
    39001 tcgctttacc ggcatgcgat tccgatcctt caaaggacaa aatgcgggca ttcatatttt
    39061 ttagccgctg ggtatcgggt tcggttactt gccagtcgcg attatcaaaa gcataaaact
    39121 tgtcttgaag tccttcgaac aaataatcct gtcgaccttc ttcggagcga aagatgggga
    39181 cgattcccag attacgctta tcgcgctgac tgatatatcc tacctcaaat tttttggcca
    39241 tgagttgaaa cgaatggcag ataccgaaaa agtactgctt gttttcgtgc gaaaggttgt
    39301 gttccaatat ttgatctaag agcgttgaaa acttttcttc ccagtcggtt gccgtttctt
    39361 cgagcgggct tcccgggccg ccgctagaaa tataaatgtc gtatccgttt tcgggcactt
    39421 cattgcgatc gcgcacatga aaatgatcaa cctgaaaatc gaccgcataa ccgttgaccc
    39481 ggacgtaata cgtaaaatta tcaataatac gtttgatatt gcgaatgccg agattgcgca
    39541 ggccgttatt catatctaaa atagcaatgc gtatcgtgac cgaagcgtgc cagtccagat
    39601 gctgttcctg attgagagag cgtgcgagtg aatctgagat catacgttta tccggcggag
    39661 ggttgtggtg gtttggcaga agcttgaaag cgcgcgctga tttttaatgc ataatacaaa
    39721 atcgctacgg cgataatgac ccacagaatg gaccacacta tggccggtaa aaaaaacaat
    39781 tgcgccacgg tgtccgcatc cgaatggccg agactatagc ggcttcgtgt cgcccccata
    39841 gacagacgta ccagagtcaa tgcgtcgctg acggcattaa gcgcacattg aacggcgaga
    39901 aaattaatta caatatagtt gagccatgta ccgcctttga aagcgagtaa aagcaagacg
    39961 gcgcataggc ctccgctaac agctaatgta aaaaaatcac gtacataaaa aacaatggct
    40021 acggcaaacc cgataccgat cacggctaaa ataactcgcg ccagatttcg ccggccactc
    40081 atgatgatca aaatgcctgc gaataatgct gcgcccagat aacccgcgct tgcgatgaaa
    40141 ccttgcgcta atcttccgcc ggaaacggtg tgttgcatat aaccgctggt atcaggattg
    40201 atgacaatgc gactgaccga ttcgcccatg atcaggctgg cgatggcgtg accgctttcg
    40261 tgaatgaatg tggtaaaaaa ctcaaacggc agaaggatca tgccggtaaa cgggaaaaaa
    40321 tctagtgcga tagataccgc gacaagagct aggattaaac cgttgctgcg aaaaaaactt
    40381 ctaccgttag gtgaagattg gggtggaccg gcggggccgg cttcggttgt atgtgatgtg
    40441 gtttcttcca aaacacagaa acttacattt tttttatatg gcgtgcaaat gaatgttgaa
    40501 aggaaaccat atttagagac cggtggaaac aaatatatag aaacacaatg atggataatt
    40561 tgaacggcta ctgatcatcc gggcaagcat taataatacg cattggtcgt gatcggagca
    40621 gtcgtatcgc ttcgtcgccc gagcgaatcc acaatgtgtc agcttgacca aaaaaatcat
    40681 acaccaaacg gttacccaat cggtctgtca tgacgagttc atccgacgaa atatgatatt
    40741 gaccgcacat aaaaacgtgt acgaccgagt cttgcctacc atactgaaaa gcgccgtttt
    40801 tttcaaatat ccagcatgtt ttacgttggg tatccgacca aaagccttcg atgtttttta
    40861 aagccggatc cgctgcgatc aaatatccgg tatgtttaca agcgatacac atcaatacta
    40921 tcattactat cagcagggtg tggtttttca taaagcgttg atcttagccc atgttttgtc
    40981 gtcccacgcg tctaatgaag caagttgaat atcggcaata caccactgcg gtttgcccgc
    41041 tgccgcggta tccggtacgc atgcggccaa catacgggcc gctttagcag cgatcacgcc
    41101 gctaagtgaa tcttcgatga cgaggcattc tgtcgggttg acgttgagcc gctccgctgt
    41161 cgtgataaaa attcccggat gcggtttacc gtagggttca tattctgcgg agtggataac
    41221 ttcaaaacga tctttgattt ttagatgagt aagaacggcc tggataatgc gcatcgcgga
    41281 cgatgaagct aaagccaatt tgccgacgtg ttttgtaaaa aaatccaaag catgatatac
    41341 gcccggcata actttgccgt gcgttgtgga tagggctatg acgcggtcca ccacgcgctg
    41401 ctctacggta taaagatcaa caccgctcca tggtttacgt ttataccaat agtgaacgac
    41461 ttcatcaaac cgccaaccca tcgtgcgttc gcagtccgca tccgttagag cgatacccaa
    41521 atcagcgaat atttcgcgtt cggcttgcct ccaaaagggt tctgaattga tcaaaagccc
    41581 gtccatatca aaaataaccg attttatcat aacggagttt cctacaaacg gcttttttta
    41641 aatttatctt gcagtttttt atcggttaat attttcaatg catcgggtgc atccgtatga
    41701 tcgagcacga ccggcccgat ttgtgtcacc gcaaaaagcg cttctttatt gagccgtgga
    41761 gatgtttggc cgatagcgat gacagcttta ttcatggctt cacgagcaaa attgggcgat
    41821 tcgtgaatat cacgtacgat attccacaaa taagccatgc aatcggcttc actaatggct
    41881 ggtccggata gtgccaaaat cgtataaccg caccgacgca ccatttcttt tggggatgaa
    41941 gtccagcgat caatccattg ttttcgatcg gttaagtcgg gagtgatgct ttcgattaac
    42001 gcatccgcaa catgagggtg ctgtacagtc tctatacggt gctgtagaag attaatatcg
    42061 ggtgaagtat ggtgtgaggt cattgcaacc aaaatttgtg cttcaggtat attggtgttc
    42121 cataagtttt ccaacaaagc cgcgtcgtta gatattttct tttgaatttt tttgagtaat
    42181 gcgagcttga ttcgcgattg gatcaaatcg gggggtacgt gcgcaagtgg ttggcgcgcc
    42241 tcgttttcgt gagcctggca taacaaagtt ataacttcat ccggggtcat gtggccttct
    42301 taatcataaa attctatgtg atcagacgtt tgggattatc cgttataacg aggcagttcg
    42361 atgatgaaaa cggcaccttc accgggtgtg ctttcgactg aaatttggcc atcatgcagt
    42421 tcaacaagtt gttttacgat cgccaatcca agaccggtgg atgattcacg gccggttggt
    42481 ttggccgaaa ggcgctgaaa tttaccgaac aattttcgtc tatcttctaa tgtaaggccc
    42541 ggcccgcgat cacgtacaga gaagcgaata attgtatccg tagcttctac cttgatatct
    42601 atttctgaat cgcgcgggct gtactttatc gcattattga caatattttc aaacgattcc
    42661 ctcaaacgaa tcgcatcccc ccatacatac gaatcagcat gcggatgaaa gtgaatgcgt
    42721 tgcgcttttt tttcggcatg gatcaggttg tcttgtataa ccaaagcggt tatttctgaa
    42781 acgtcggttt tttctttgtt cagtttaagc ttaccggatt cgatgctcat ggtctcaagt
    42841 aatccgttta taagatgaag catctgtgaa gacgatcgct gaatctgatc aaggtcttta
    42901 ttggcttttt cgggattgtt cagttcgacg cgaaccagat cagtataacc gacaattcct
    42961 tgaagtgggt tgcgtagatc gtgtgcagcg attcccaata actgcgattt ggtttcgctt
    43021 gcttgttgag cgatttcccg ttccctttcg gcaacacgcc gtgcggattc ggccatttgc
    43081 aatgcgtttt ctgttctttc tttttcttct tcgagtttac gcgtacgcat ttgtaccaaa
    43141 gcggccagtt gttgttcgcg ggcgatcagc tgacgtacgt tgtatcggta tgcacccaac
    43201 acaacaaaaa caactccggt caaacacaaa agtacaaacc accatgtttg gtaaaaaaat
    43261 ggttgttgca caaaatccag gcttgcgccc tgtgtattcc aaattccgct ggcattggcg
    43321 gcaatgactt gaaaacggta cgtaccgggc gggatattgg tataaacagc cgttctccgg
    43381 taacctgcat caatccagtc attgtcataa ccatctaact taaatttgaa acgaatttta
    43441 ccgggtgcac tataactcaa accggtataa taaaattcaa tgcgatcgtg ccccgcagga
    43501 atgaatatag tcgaatccaa tctgatattt tgttgagcgt cggcaaatcg agtaatgaca
    43561 acgtaaggag gatcaaaatt ttttacaatt ttggaaggat caaccacaga tacgccatta
    43621 atggtagcaa accacagccg gcctgattta tcttttatcc cgccgtattg tgctccgcca
    43681 ttgcattcat tggttttcat gccgtcatca atcccgtaag catggctttc cagtttcgtt
    43741 ataatacgac ggtctaattg ttcaaattga tgtttattga ttctgaaaat gccgcgattg
    43801 caactcatcc aaaaattggc actgtcatct tctaatatag aaaacacgac gtcatcaaat
    43861 aatccgtcac gacttgagta gcgcttaaaa tgcccgtcat gataacgaat aatccccccg
    43921 ccgtgtgtac caatccacat atcaccgctt tcttcttcgt aaaaagtcat aaccgagtgg
    43981 ctcaccgtct cgtccggcga cttaaaagat tcaaatttac cgttgcggag aatattgacg
    44041 ccgctaccgg tgcctatcca gagccctccc ttgcgatctt cgtgaagagc acgcacaata
    44101 tcatgattca aaccgttaaa tgtcgtgtaa acggaaatat tattatgatg taacgcgtgc
    44161 aggccgcctt tggtgccaat ccacacgatt cccgaacggt ctttcaatac aacgttgata
    44221 aagttattgg tcaaaccgtt agttttatta tataatttat attttccgtc ggaccatcgg
    44281 gtcaatccac ccccgtctgt tccgatccac atcgcaccct cggggtcttc atataatgca
    44341 tgtatgggaa agtttggtaa cccattggtt cccaaatgaa gtgatgtatg ccttgtgttt
    44401 tgattaccga agtcaagcat gcttacacca agatcggtac caagccatac ccgatttttt
    44461 cgatccacaa ataaacattg gattacgtta tccaaaatgc cttcttcgtt gccatacgta
    44521 ataaaaagcg catctttgaa acaacttaag cccccggatt ccgaaccaaa ccaaaaaata
    44581 ccctcgcggt cttcataaag tgaaattatc gtattattca tcaatccttc tttggcgctg
    44641 atgagttcca tgtgtttttg attccatcgg aataatccta atgaagatcc gagccataga
    44701 ttgccgtgac gatcataccg gattgcatat atctgagtgt tgtttggaat atcaaattcg
    44761 gaataacttt ttaaaccata agattgatcg gtttgttcaa ccccaaaaaa accgttgttt
    44821 gttccgacta aaatgcgatt gtctttgttt atggataaag cgtggatggc gattttgttt
    44881 gagatattct tgtttatgga atccacaata atgatttttt tagaaataga gttaatcatg
    44941 tataacccat cgtgcgaacc aacccaaatg tttccgttta gatcacattg aagtgcatga
    45001 atgatagatt cggatgaatt atacggtaaa aaaacgcgtg ataacgaatc gccattggta
    45061 tggtacaagc ctctgtctgt tgcaatccaa atacgtttat cgtggtccat ggtaatggcg
    45121 ttgacaaacc gttcgctcaa aggggaaaac gaagcggtac tataaaaacg attgttcttg
    45181 tgtatgacaa taccgccgcc gcccgttcca atccataacg agctgtcggg cattacggtt
    45241 aagcttataa tgctgtggga tataaataca ggttcggtgg ttttattgaa tacttcaaac
    45301 tggattccgt caaatctaac gaggccttcg aatgttccta tccataagta cccgtccgga
    45361 gtttgtacga ttgatgttac cgttgactgg ggtaaacctt ctttcaagtg ccaattggta
    45421 tgcgcgtatt gagtcaatcg gcgcgcagga tcaagagcat aagaaaaatc taatcctaca
    45481 aaaacacata cgaagaaaaa aagatgtaca gatttaacaa gaagtgcatt catatgtcaa
    45541 agggtgtgag ggcaacagaa ctgaaaattg cggttagcaa gttaaacgct gatccaaatt
    45601 agaagtattt ctacaattat tcaaatactt agaagtaatt ttttgcattt gcatgtcgag
    45661 catgatagat tggtgccatg tcattatttg aaatagacaa atcaggattt gaacaacaca
    45721 cagcacgcgt accgcttgca gaacgcatca ggccgcgcac gctaagtatg gttatcgggc
    45781 aagagcattt gcttgggcac gggaaagctt tgcgtaccca gatcgaacaa ggtaatgtcg
    45841 tatctatgat tttctggggt ccgccgggta ccggcaaaac tactttggcg cgcgtgatcg
    45901 ccgcagagac gcggtccaag tttgtttcgc tgagtgccgt ggaggcgggt gtagcggatg
    45961 tacgtcgggt cattcaggat gcccaggcgc tcaaacggca gaccggggaa cgaatgatgt
    46021 tgtttattga tgaaattcac cggtttaaca agtcgcaaca agatgccttg ctgcatagtg
    46081 ttgaagaagg aactatcacg ttgatcggcg cgacgacaga gaacccttcg tttgaggtca
    46141 tcaatgcttt acaatcccga tgtaaaatat acaagctgga atctttgacg gatgcacatc
    46201 ttgaaaagat tttggatcaa gcgttaaaaa cggatgacct tttaaaaact ctgactgtcg
    46261 aaatcgccga tcttcacagt ttgatttttt acgccgccgg cgatgcccgt aatttactca
    46321 acacgttgga attagccata cagatcaccc gaccggatac gaacgggaaa cacattgtta
    46381 ctcgggagat cgttgaagag gcttatcagc agcgtaatat tcattacgat cgggccggcg
    46441 aatatcatta tgacacgatc agcgctttta ttaaaagcgt ccgcgggagc gatccggatg
    46501 cggcattgta ttggatggct cgtatgctcg aaggcggaga agatcctaag ttcatcgctc
    46561 gacgattgat tgttttggcg tcggaagata tcggcaacgc cgatccctac gcgcttacgc
    46621 tggcggtttc gacgtttaca gccgtagatt acataggcat gccggaggct cgcatcgtcc
    46681 ttgcgcagtg tgttacgtat ctcgcctctt gtcctaaaag taacgcatcc tatctggccg
    46741 ttgacgccgc tatccatgat gtacgatcag aaaaatcaca tcctgtaccg ttgcatcttc
    46801 gtaatgcgcc taccaaactg atggaaaaaa tggattatag taaaggctat aaatatgcgc
    46861 atgatttttc agatcagggg gagccgcact ttgttgaaca aatatattta ccggatgatt
    46921 tgaaggcgcg catttattat cagcccacag ccaacggcgg agaaaagcgg ttaaaagatc
    46981 ggttggaaaa aatctggaaa aaacgaaata aaaaggattg agttatgcct gttgttaaaa
    47041 ttcaaaaact tcatccggat gccaaacttc cttcgtatgc gcaccccggc gatgcaggtt
    47101 tggatgtttg ttcagtcgag agggtagaaa tattacccca tgcttcagct ttgatcaaaa
    47161 caggtttttg catgcagctt cctgacggca cagaagcaca ggtgcgacct cgaagcggtc
    47221 ttgcactcaa acatcaaatc accgtactca attcacccgg tacgattgat cacggttatc
    47281 ggggtgaggt gggtatcatt cttatcaatc acgggtcaac tccttttata gtagaacccg
    47341 gtatgaaaat cgcacaaatg gtcatcgcag aagtgctgca cgcacatgtg gaggaggcga
    47401 cggagctcac ggatagttcg cgcggtcagg gcgggtttgg cagtacgggg cggtagatat
    47461 gtcaaactta tttcaaatac atcgtttggg tttggtggat tatcaagtcg cttgggatct
    47521 acaaaaacaa ctttttgatg attgcctaag tggccgaaat ggtgatgcga ttattatttg
    47581 cgaacatcct catacatata cggttggtaa aaacggtgtt gagagtgttg gaaaacattt
    47641 attgcttaat caggaacagc tccgggaaaa tgggattctg gtttttgaaa ttgatcgtgg
    47701 cggcgatatc acatatcatg gtccgggaca aatcgtgggg tatccgattc tcaacctaaa
    47761 ccgttattac cgcgatgtcc accgatattt gcgtgacatc gaagaagtaa ttattcgtac
    47821 gatccgtcat tataatattg taggaaaacg aattgaaaag gttaccgggg tatgggttga
    47881 tacgccgcgc ggacctgaaa aaatctgtgc gatcggcgtt aaagtaaccc gatgggtcac
    47941 gatgcacggc tttgcattga atgtaaatac aaatctcgat ttttttaacg gaatcgtacc
    48001 ctgcggtatc acggacaagg gcgtaacatc catagcaaaa ttgctcaata aagaacagga
    48061 tatcagagaa atagaaaacg tgatcatcca tgaatttcag tccgtattta aagctgccga
    48121 aatgccaata atggaaaatc aaggagcgat atgaaaaaat atatcttttg ttatgtggta
    48181 ataatatctg aagtgttggg tcggtggggc ctggctcaaa atccggatca agccaaatcc
    48241 tggtatgata aaggacgatc cgcgtatctt cgttttatgt cagacgacta caagacggca
    48301 ttgtcttatt atgaaaggtc gattgctgcg gattccaact acgcaccggc ttttgccgga
    48361 aaagcggagg cgttgacgct tttagctttg gaaaaagaac aaaatggttt ggcagcagaa
    48421 gctgatttta cccatgcgtt acgatcagcc caacaagcga tagctaaaaa accagaccaa
    48481 aacttaggtt accgtgctct tgcgcaagta tatctgaata tggactccaa aacctacggc
    48541 gaaaaagcgc atgatgcgtt acagcgtgct atagagttgg attcaaccga tgcggaaagt
    48601 tattttttat tgtggcttct gaccgaaaac gacaaaccaa acagcccgct tttgcgatta
    48661 tcattgaagt tagatcctaa ttcgtttcaa actcactatg ctttaggtca atactggtct
    48721 cgtaataaac agccggataa agcgatagac gagtacaagc tatgtactcg gattaacccg
    48781 gacaattacc gctcttatta ttcacttggc aatgtttact cacaaatcaa acgatacgat
    48841 atggccgcgg aagaatacga gaaagctatt gctataaata aaaacctgta cgaagcgtat
    48901 ttttatgtcg gtgttgcgta ttattattta gataaaaaca aaaaagcaaa acaacgactc
    48961 tctcaatata tagagatgca acctaatcct aaatataaac tgcaggctga gcagcttctc
    49021 atggatatga aataaaacgc tccaatcgtg aaaacagaag aatgataatt aattttatta
    49081 attttttgat tttcattcaa atacctcttg acatcggaac cttttttttg tatcattgca
    49141 acccgctgat gaaatggcga aaataagaag cggcaggtgt taataaattg ttaattgttc
    49201 gccaaatgtt catagaaatg agatttttga ttctggtaaa taagttttta aaaaaactta
    49261 ttaaaaagta aaaaaagact tgcaatcaga aacaaatttg tttatactta cgtcccgcag
    49321 ttgaaaaagc ggtttgttcc ttaaaaaaca agactatagc tcgcgaaagc gaattataaa
    49381 ttatattgct gagaccttga gtcgaagccc aatgttgttt gaaaatgaga gtatacgtcg
    49441 atcaattcat ttattgagtg attctggtca agaataacca tcttctttgt taataacaaa
    49501 aaaagatatt gacaacgaag agtttgatcc tggctcagga ctaacgctgg cggcgtgctt
    49561 aacacatgca agtctaacga gaaagcggta gcaatatcgt gagtaaagtg gcgaacgggt
    49621 gagtaacacg taaccaatct gccttgaaga ggaggaaaac tgagggaaac tttagctaat
    49681 cctccataat gtggtgccta tcgcatgaga acatcattaa agatttactc gcttcgggaa
    49741 gaggttgcgt ctgattagct agttggcggg gtaaaggccc accaaggcga cgatcagtag
    49801 ctggtctgag aggatgatca gccacactgg gactgagata cggcccagac tcctacggga
    49861 ggcagcagtg aggaatattg cgcaatgggc gaaagcctga cgcagcaacg ccgcgtgtag
    49921 gatgaagtct ttcgggacgt aaactactgt tagaggggaa gaaactccgg ttacgccggg
    49981 ggtgacggta ccctcagaga aagccacggc taactctgtg ccagcagccg cggtaataca
    50041 ggggtggcaa gcgttgtccg gaattactgg gtgtaaaggg ttcgtaggcg ggatgataag
    50101 tcagcggtca aatccgacag cctaactgtc gactatccgt tgatactgtt attcttgagt
    50161 acaagagaga tgagtggaat tcccagtgta gcggtgaaat gcgtagagat tgggaagaac
    50221 accagtagcg aaggcggctc attagcttgt tactgacgct gaggaacgaa agcatgggga
    50281 gcaaacagga ttagataccc tggtagtcca tgccctaaac gatgaggact aggtattggc
    50341 tcgcaagagt cggtgccgca gctaacgcat taagtcctcc acctggggaa tacggccgca
    50401 aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc agtggagtat gtggtttaat
    50461 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccg atgcatagtg cagagatgca
    50521 tgaagtcctt cgggacatcg agacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag
    50581 atgttgggtt aagt
//
LOCUS       DAOQGF010000008        46425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_313_length_46425_cov_6.369722, whole genome shotgun sequence.
ACCESSION   DAOQGF010000008 DAOQGF010000000
VERSION     DAOQGF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 46425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_46425_cov_6.369722"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="tpiA"
                     /locus_tag="PK937_02585"
     CDS             complement(<1..635)
                     /gene="tpiA"
                     /locus_tag="PK937_02585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNH31491.1"
                     /translation="MRKKVIVGNWKMHKGPNAALHLAKSLKLKLTDIRKTEMAVCPPS
                     ISIAAVRDVLKDTKIKVGAQNMYLHKYGAYTGEISAEMIKESGCEYVIIGHSERRQHF
                     FETDTTVNHKIEFALQELLTPIVCVGETLEQRQEGHTESVIRQQYLGAMKNLDSDKAA
                     KIIIAYEPIWAIGTGKNATVAEAEAVHKFIRDLARESFGKTISDKMIIQYGG"
     gene            complement(628..1824)
                     /locus_tag="PK937_02590"
     CDS             complement(628..1824)
                     /locus_tag="PK937_02590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNH31492.1"
                     /translation="MNKLTIDQVHVAGKKVLVRVDFNVPLDDTLRITDDTRIRESLPT
                     IKKILSDGGIPVLMSHLGRPKGKKNPAMSLKPCAVRLSELLGQPVIMAEDCIGDTVEK
                     QAAGLSKGQVLLLENLRYYNEEEANDIAFAEKLAKLGDIYVNDAFGTAHRAHASTEGV
                     TRYFKQNIAGYLMEKELRYLGNAVQNPKRPFVAIIGGAKISGKIDVIENLMKKVDTII
                     IGGGMAFTFAKVMGYEIGNSLFEAEKADLAKSIIERAKSGTTRVLFPVDAVVATEFKN
                     EAEIKTVSMDQIPSGWMGLDIGPVTIDMFRNEINSAGTVVWNGPMGVFEMPNFAKGTK
                     AIAESLVEATQKGGVTIVGGGDSAAAITELGLEKQVTHVSTGGGASLEFLEGKILPGV
                     EALTHA"
     gene            complement(1840..2901)
                     /gene="gap"
                     /locus_tag="PK937_02595"
     CDS             complement(1840..2901)
                     /gene="gap"
                     /locus_tag="PK937_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNH31493.1"
                     /translation="MAIKVGINGFGRIGRLVFNALVDKGLLGKEIDVVAVVDVSTDAD
                     YFAYQLKYDSIHGKFKGTINVAKSKPDLDHNDILIVNGDRVQCVAATKDPSQLPWKDL
                     GVDYVIESTGLFTDSEKAKGHLTAGAKKVIISAPGKGDVKTIVMGVNEQEYDASKHHI
                     ISNASCTTNCLAPLVHVLLKEGIGIETGLMTTIHSYTATQKTVDGPSKKDWRGGRAAA
                     INIIPSTTGAAKAVGEVLPATKGKLTGMSFRVPTADVSVVDLTFRATRDTSIEEIDGL
                     MKKASETYLKNILGYCNEELVSTDFIHDERSSIYDSLATTQNNLKGEKRFFKVVSWYD
                     NEWGYSNRCVDLLMKITKA"
     gene            complement(3005..3553)
                     /locus_tag="PK937_02600"
     CDS             complement(3005..3553)
                     /locus_tag="PK937_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNH31494.1"
                     /translation="MSTYIALLRGINVSGQKLIKMNDLKSYFVSYGYKNVQTYIQSGN
                     VVFESAKQESEKLSKKVGEQLAVSLGYIVPVIARSLNEWVIVNENNPYAKKKLRPDER
                     VYVTLLAIKPKPDLAQSLNALSDTKDEFQLNGKTVYILCRKGYGNSKFSNNFIEKKLD
                     TQATTRNLATMQKLQMIAEGLI"
     gene            complement(3572..4144)
                     /locus_tag="PK937_02605"
     CDS             complement(3572..4144)
                     /locus_tag="PK937_02605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010111456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNH31495.1"
                     /translation="MIKILAISGSLRKNSYNSALLRAMIKLAPENVSVELFYGLGELP
                     LFNPDIEDQAFPSVLHFRNQLASHDGIVIASPEYAHGVTGAMKNALDWVVGSGELVYK
                     PVAVINAAPHATYAYASIIETLRVMTADIDPVASKVLPSAGQKLSDDEIYTHKEISNA
                     LRSTLVSLAQTIRSRDAATSSEKNASWNLF"
     gene            complement(4141..4821)
                     /locus_tag="PK937_02610"
     CDS             complement(4141..4821)
                     /locus_tag="PK937_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNH31496.1"
                     /translation="MRSPKQLFQILSLLLIAALTAVIFFIHRIHDKSKQISENWLPSV
                     MHVGNLNTLTSDFRIMELQHILSTSDDQMRTYESAMQRIADLINSEMKLYEPLITTSV
                     EKTLYEDFLNRWSEYMKHHIDMLQLSKLNKNEDAKQLIRARSEVLFNEYSSALSALVH
                     ENRRAADDEVKRANALYIGSVINLILILGCVAVFVVISLRQMRKIFDRVLSTAMDVVA
                     KERDGKSI"
     gene            5009..6403
                     /gene="purF"
                     /locus_tag="PK937_02615"
     CDS             5009..6403
                     /gene="purF"
                     /locus_tag="PK937_02615"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNH31497.1"
                     /translation="MSEDIKEECGVFGIYGHPEASRLTYFGLYSLQHRGQESTGIVSA
                     DGDRTYRHVGMGLVSDVFADDKIFDRLPGHSAIGHNRYSTTGGSFMQNVQPLTAIIST
                     GPISAAHNGNLTNYQSLRRELQNNGAILQGNNDTEVILHLAARSGKTTVEEQIIAALH
                     QIRGAYSLLLITKDKLIAARDPWGIRPLCIGKLDQATVIASESCALDIIGAEYVRDVK
                     PGEFIVVDHDRMQTRMIFDPLPTPAHCIFEFVYFSRPDSKIFGDNVDKARRKLGKRLA
                     LEHSVDADIVISVPDSSNTAAVGYSRRTGLKFELGLIRNHYVGRTFIHPVQSMRDLKV
                     RLKFNAVEGVLRDRRVVVIDDSIVRGTTLKALVTMIRKAGAKEVHIRISSPPVTSPCY
                     YGMDFPTKEELIANTMNKDTLCKSIGADSLEYLSLDGMLECVPNGPHNYCTACFSGKY
                     PVPIEPDTQITQCD"
     gene            6607..8592
                     /locus_tag="PK937_02620"
     CDS             6607..8592
                     /locus_tag="PK937_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNH31498.1"
                     /translation="MSEHSSQGVKGLPSNAYTELKPGETYTPIMSPQQVYPEVNFRSV
                     FWGLIMAMLFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSAASKRKNALGENVIIQSIG
                     ANSGAVVAGAIFTIPALYILNLQADFFKVFLASLFGAFLGTLFLIPFRKYFVADMHGK
                     FPFPEATATTEVLVAGEKGGNQAKVLVLSGIIGGIYDFIVATFGWWSEVFSTRALPWG
                     MELADKAKLVFKVNIGAAVTGLGFIIGLRYSLIIACGSFVSWYLLIPTVYYIGAGLTT
                     PLGANATKLIGAMTAEEIFRNYVRHIGIGGIAMAGIIGIIRSSGIIKSAFILGFKEIG
                     GNKGDNANALRTQRDLGMKIVGIGILVVAVMIFIFFQMGVVQNLTHALIGLAVVLIIS
                     FLFTTVAANAIAIVGTNPVSGMTLMTLIIASVVLVSSGLTGEVGMLSALIIGGVVCTA
                     LSCAGAFITDLKIGYWLGSSPYKQETWKFAGALVASLTVGWVIMLLNETYGFTGPDAL
                     VAPQANAMAAVIQPLMTGQPAPWMLYVGGAFMAIVLAWLGVPALAFALGMYIPLELNT
                     PLLFGGLVAHWVSTRSTDEKLNNARRERGTLIASGFIAGGALMGVISAILRYAGYNWF
                     NAEWAESHGGELLAGLMFVGILIYMYWDSMRGKPEEE"
     gene            complement(8653..8727)
                     /locus_tag="PK937_02625"
     tRNA            complement(8653..8727)
                     /locus_tag="PK937_02625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8690..8692),aa:Pro,seq:ggg)
     gene            complement(8769..9929)
                     /locus_tag="PK937_02630"
     CDS             complement(8769..9929)
                     /locus_tag="PK937_02630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH31499.1"
                     /translation="MQYPFSSLSQTLKGSPTVRLAEKAQTLKDAGADIVDLTIGEPDF
                     PTPAHISEAAYRAAQAGNTKYTNSRGILPLRKAIAEKLQRDYSLSYDANTEIIVTPGG
                     KQAVVYVIQAIINPGDEVICIEPCWVGYEGSIAMSGGRYIGVPTYESDSFCIDIDRLR
                     AAVTPKTKLLIINSPSNPTGKILSKREIESICSVCNERNLMLLTDDIYEKILYDNHMF
                     VPAAGLPGMRDRTIILNGFSKAYSMTGWRLAYLAAHKQIISEINKLQQLSATCPSSVS
                     QWAGVEALNGDQSAIQVMTSAYAVRREIVVQGFTKYGLPCFRPEGAFYAWINVKKLGM
                     NSVAACEYILDKCNIAAVPGSAFGISGEGYIRASFATSEENLQKAIKNLENL"
     gene            complement(9929..10525)
                     /locus_tag="PK937_02635"
     CDS             complement(9929..10525)
                     /locus_tag="PK937_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNH31500.1"
                     /translation="MGMQAKPAVFFDRDGTLNVEKNYLYRPEDFEWISGVPEAVHMLN
                     EAGFLVFVITNQAGVARGFYSEQDVNRLHDFMADQLEQKKAHMDAFFYCPHHPEGTIH
                     EYTKLCDCRKPASGMIRQALMRFDIDLSASFVVGDYKSDIELANREVIRSVLVKTGHG
                     QKTIDAMNAGSTACKPDHIAEEVTEAVRWILGQHRERK"
     gene            complement(10527..12257)
                     /gene="recJ"
                     /locus_tag="PK937_02640"
     CDS             complement(10527..12257)
                     /gene="recJ"
                     /locus_tag="PK937_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNH31501.1"
                     /translation="MLEQQWVLSNHHDKERVKKLAEEINVPELIAGILFNRGIRTFEE
                     AKNFFRAESAPLNDPFLMHNMDIAVDRVTRAINNRESIMIYGDYDVDGTTSTSMLYLF
                     FKEIHRGFISYYIPDRVKEGYGLSPTGIKYAQEQGIDLLIAVDCGITAIDQVNLANKY
                     GIDVIICDHHHPGDQLPAALAILNPKKESCPYPYKELCGVGVAFKLAQAITQTLKIDE
                     DVLMQHLDLVAIGSAADIVPLTGENRTLVKNGLKLVSDANKVGIRQLVKNANVFGKPI
                     STAQIVFSLAPRINAVGRLGDANRAVKLLVTKSESEAVEFASVLESENRNRRDLNEVM
                     FLEACEIVDQEFDFANDKVIVVYKKGWHQGVIGIVASKLVERYYRPAVVIAEADDKGK
                     GSARSVENFDVFQALKSCEDLMVGYGGHKYAAGLTIDLNNIEEFRRRLNEYAAIHLPP
                     NDLIPKIHIDAEISLDEINNRLMTLLNLFKPHGPANMRPIFISRSLQIVGYPQLMNER
                     HIRMKVRQNGAVFDAVGFNLGHYYNKIGVGDNPLDLVYQIDENTWQGRTTIQLKIKDL
                     KIQTNGNGYH"
     gene            12463..13038
                     /locus_tag="PK937_02645"
     CDS             12463..13038
                     /locus_tag="PK937_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31502.1"
                     /translation="MFKHRVARNRFLYVILLGLIITGFGYLMINYQLKMYQMVLMAAM
                     ILIPNRLARYYWFDFFAGKRELRRKRPEQATIRFRQFLETLRGAPWIKWLMFFSYGLY
                     SFKVEAVALVYLGHAWLYLKKYDDAEKAFRDALLVDDKYVMAKRGLAAVYILRGQNNE
                     AQQWLDLACRFGHPKISFDKFEELTRKEYNA"
     gene            13035..13898
                     /locus_tag="PK937_02650"
     CDS             13035..13898
                     /locus_tag="PK937_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNH31503.1"
                     /translation="MMRVWKLIVDYGRMIKFSHTIFALPFALTSFVVSGREFPLTLSK
                     FFWIVVAMAGARSAAMGFNRIVDARYDAKNPRTVNREIPRGVITQKQAWIFVVIASTA
                     LVLASYFLNPLCFILSPVALFFVLFYSLTKRFTATAHIFLGIALGIAPLGAWIAMSGQ
                     WDWTAFMLGCAVLTWVSGFDIIYACQDYEFDRSEGLFSIPGKFGIANALWISRFLHAT
                     AIFFLTYVGSHWELGWIYFAGVCIIATLLIYEQSLVHAHDLSKVNIAFMNMNGIISIT
                     YFVFTVLDIYL"
     gene            13932..14306
                     /gene="crcB"
                     /locus_tag="PK937_02655"
     CDS             13932..14306
                     /gene="crcB"
                     /locus_tag="PK937_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNH31504.1"
                     /translation="MIQFILIGLGGALGAMARYGLQYVIQQRSGLLFPLGTLIVNLIG
                     CFFIGFIMEIAEIRSAFNPQIRLFITVGLLGGFTTFSAFGYESILMMRTMEFYFSIFY
                     IVGSIVGGLALVILGQTLARLL"
     gene            14448..15530
                     /gene="mtnA"
                     /locus_tag="PK937_02660"
     CDS             14448..15530
                     /gene="mtnA"
                     /locus_tag="PK937_02660"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNH31505.1"
                     /translation="MVQPIYSRQNYVFVFVKLNMIETIRWAHPAVRIIDQTQLPVYET
                     YLTIADLDDLIDAIKKLKIRGAPALGIAGAYGLCLGLYRCTTSDKAILRDEAINTAAI
                     LIQSRPTAVNLAWGVHRALKVFESVYSTASSTDILRSSVLKEAHLILQEELDACIAMG
                     KLGSELIDDGMQVLTHCNTGGLATGGFGTALGVLVTAHQNGKRIHVWVDETRPLLQGA
                     RLTSWELKKENIRHTLICDNMAGHLMSKGRVQAVIVGSDRIARNGDAANKIGTYTLAV
                     LCEKHKIPFYVAAPHSTIDKQIPDGSAITIEERSADEITMGMGKQTAPQGVHVYNPAF
                     DVTPAELISAIITEKQIFRGPDYCFN"
     gene            15576..17078
                     /locus_tag="PK937_02665"
     CDS             15576..17078
                     /locus_tag="PK937_02665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31506.1"
                     /translation="MKLVFKSVAAMILLSLILGQQIFAQEKKKISHSASIQGIKQRLK
                     NASNEPGDADKYYQQALEILLVNNAQVDKNDKEKEETHYYAAAVYHRMNDMIKAWEYC
                     NKTLTFGKKYFEKGEKVSGGIELFSIKDMMNDVKLKTYNQGNQAYRAAAAATSPDSQK
                     VLYHKTISKFKSLLEIDPTVTITANNVPSSYALGVYGTIATVYIQLLNMEKEEANKKT
                     IREDIVSYLTKMHALDRANFSFVTSIISLYTQENNEEKMMAWMDTALAMNAPDSQAVA
                     YQTSLIGDKALMLDRQGKADEAIAVYTKALEKNPENPDLHFNLGRLYYNRKDFDKANT
                     QFRKVKSLRKDDPVVNFQVAEQSYQSYQSKRTETIDKNGGARADMNKITTMLKSDIEI
                     VRTDIADAIASLEASLPTTLEMAETNYRIGKMYNYQAEIEGHLYYTLVNAEKVKKQKP
                     YFEKAIPYLVKASELRPSHANTWIMLGTAYVNLQKKREAEAAFKKANEIK"
     gene            17154..18452
                     /gene="purB"
                     /locus_tag="PK937_02670"
     CDS             17154..18452
                     /gene="purB"
                     /locus_tag="PK937_02670"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNH31507.1"
                     /translation="MITRYTRPELGKIWSDENKFQKWLDVEIAVCEAQADLGYIPGDA
                     LAAIKSRARFDTARVLEIEAEVKHDVIAFLTNVAEYVGEPSRFIHLGMTSNDVLDTSL
                     SLTIREAGNILLQDIQQLLDVLHRRALEFKMTPCMGRTHGVHAEPVTFGLKLALWYDE
                     MTRNAERLKRAVEAISVAKISGAVGTFEHLDPRVEEKTASILGLKPINATQVVPRDLH
                     ADFLVTLAVIGASLEKFATEIRSLQRTEVLEAEEYFSKGQKGSSAMPHKRNPITCERV
                     AGLARVLRGNAQAALENVALWHERDITHSSVERVIIPDSTILLDYMLTQFIRVVDKLI
                     VYPENMMRNIERSKGLIFSQPVLLALAKKGLKREEAYKIVQTTAMAVWETPDGNFKKA
                     ITTNAEVTSRLSKEEIDECFDLKHSMKHIGYLFERAGIKS"
     gene            18493..19254
                     /locus_tag="PK937_02675"
     CDS             18493..19254
                     /locus_tag="PK937_02675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF006868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNH31508.1"
                     /translation="MEVNIKKLEKEKIFLEGTIKKFYSTNNPDYLLMECKNDLVPMDT
                     KKVMKARGKGQINTELTSYVFQFLESYHIPTHYVGKYDDKSLVVRNLEMIPIYVVVRN
                     IAAGSFAKNFKMSEGDPLPAPIIEFYFKNDKMGNPMVNEYHLYAFGSANQDEVRTIQR
                     LSGKVNAIVRNFFERRGFQLVDIKLEFGRQKGKIYLADELTLDTCRLWDINRGRKFDK
                     EIAAAKENDLHKIYGEVRQTITHPKTRDERPTIHD"
     gene            19269..19913
                     /locus_tag="PK937_02680"
     CDS             19269..19913
                     /locus_tag="PK937_02680"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNH31509.1"
                     /translation="MKISREGLVTIFIVNILVSIAIIMYATLGSGWIALVIALLLVEL
                     LVLWFFRDPERTIAGDDNTIIAPADGKVVLIRDIREDKYLKSEATQISIFLSVFNVHV
                     NRYPLGGTIAFYEYMKGKFMVAFDDKASELNEQTVVGLEHPRVKILFKQITGLLARRI
                     VFYGKQGDRVDRGTRCGIMKFGSRMDIIVPKNVDIKVKVGDTTTAGETILGIIK"
     gene            19929..20675
                     /gene="pssA"
                     /locus_tag="PK937_02685"
     CDS             19929..20675
                     /gene="pssA"
                     /locus_tag="PK937_02685"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNH31510.1"
                     /translation="MKASPKRHLPSLVTALNLFAGFLSVISSIRGDYMEAASLIVVAA
                     IMDVLDGKVARLTKSSSEFGVELDSLADVSSFGFAPAVLIYRAYFEPWGIWGILISFT
                     PLVFGGIRLARFNVGVADHAEKDSYFTGLPIPSSAITIASFVAFEMDVFQACNHIFVM
                     AIVTLLVSFLMVSKIRYEGMPNFSFRKTEDRIKLGLLIVCFIAFLIYPQRVAFPVMIL
                     FIAYGILMGILAIFSNKESDDDTQDIPAID"
     gene            complement(20679..21890)
                     /gene="tyrS"
                     /locus_tag="PK937_02690"
     CDS             complement(20679..21890)
                     /gene="tyrS"
                     /locus_tag="PK937_02690"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNH31511.1"
                     /translation="MFLPIADQLAVIKRGIVDLIPEDELVKKLENSLRTAKPLKVKLG
                     CDPSRPDLHIGHSVVLRKLRQFQDLGHQAILVIGDFTGMIGDPTGKNKTRPSLSLEET
                     RLNGKSYLEQASKILDAQKTKIVYNSDWLGAMNFADVVKLASRYTVARMLERDDFENR
                     YRSGEPISIHEFLYPLAQGMDSVALEADVELGGTDQRFNLLVGRDLQREYQQQQQVII
                     TMPLLEGTDGVQKMSKSLNNYIGIDDSPKEMFGRAMSIPDALIYKYFELTTNVDAAEL
                     HKIKTDLDSGTINPSHLKRRLAKELISIYHDDGRAQAAEEEFDLIFKKKEIPEDIPVI
                     SLNKGTWIISKLLLECKLVESGKEAQRQVQQGAVSINGEKIADDRAVIQIESEIVIKV
                     GKRKFSKIIPT"
     gene            complement(21890..23032)
                     /locus_tag="PK937_02695"
     CDS             complement(21890..23032)
                     /locus_tag="PK937_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31512.1"
                     /translation="MKPTLMLVDDESRVLEVLVKTFESQYQIFSFTGAHDALELLKRQ
                     PVQVIITDERMPKMNGMAFLKEAKVLAPNTVNIILTAYTDINVAIEAINSAVVYRYVV
                     KPWDTDDLRAIVRQAFERYALVQENKKLTDELITKNAALEQNLKQLKETQEKLLRQEK
                     LAVVGQLTASIGHELRNPLSRIKASAALMRNEILPEEKEKKELLQIIDNEVMISTKII
                     NDLLDYSRDRKTIFKPGNFNIIIDETLMRMRFPDEVEVVRELAPDLPDNQVDEGQIQQ
                     ILINLMLNAIQAMEYSGKIYVRTHHDGDAAIVEIKDTGCGISNENLERIFEPLFTTKP
                     KGIGLGMCIVKMLIEKHGGTIGITSRENIGTTVKIRIPFHPNFMEN"
     gene            complement(23029..24621)
                     /locus_tag="PK937_02700"
     CDS             complement(23029..24621)
                     /locus_tag="PK937_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH31513.1"
                     /translation="MKKIAGNERADYNMMAEQQMYSLSEVLNALRMRAKPETFASVWL
                     QEWLRQCDVVRLECFGTNNDGQWSSILRWEKNGTHNPALNSQPAHPNAAMQIAFKQAE
                     PLIGDSSQIYGKASAVPMTSIVLPVLSDKQVVGLIVADKLTEPEKSINIPFLELLSSL
                     WGLHRASLDATETLETVRFERDNLRAQIEQTLAAVKPETPQDELARTMITIARSLAQM
                     LLPSITQQDKNNIDAVLAFSKEVSMYEKNELHTTYSESQDNAESDISSVLDESVDIAR
                     NDETCVKKRFIIDFKRQDREIVWSEFKRLQQFISSVFSLMIGYGKENSTITVEYTPHG
                     EFLKILIKVDSEREFNEKIGDFYTFTARLNGWECDWSAHENRIQCILSLRRITGSDSS
                     KKNSQSLSDPKKETRLLIVDDDESLRELMVDILESRNYEVVCCPDASSAMEQINKLRY
                     DMIITDLGLPNISGSELAAQIKALNPHLPVMMMTGWNIDTERPDARTTYVDFILPKPF
                     NLNELLEKVENGLHASRTMRHS"
     gene            complement(24599..25156)
                     /gene="dcd"
                     /locus_tag="PK937_02705"
     CDS             complement(24599..25156)
                     /gene="dcd"
                     /locus_tag="PK937_02705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNH31514.1"
                     /translation="MILTDTQILAEIAKGTIRIEPFRRDCLGSNSYDVHLGKYLAVYS
                     EETLDCKKDNPIEHFEIPEEGYVLMPTKLYLGVTQEYTESMAHVPFLEGKSSIGRLGI
                     DIHATAGKGDIGFCNTWTLEISVKHPVRVYEGMPIGQLIYFEVSGQIETPYNSKPSAK
                     YNERTIRPVASMMFKNFDREKNRWK"
     gene            25268..26368
                     /gene="mutY"
                     /locus_tag="PK937_02710"
     CDS             25268..26368
                     /gene="mutY"
                     /locus_tag="PK937_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNH31515.1"
                     /translation="MTSQRTKTQTPEKITKNLLLWFYKHKREMPWRTTQDAYRIWVSE
                     VMLQQTQVEQVRPYYQRFMVAFPDILTLANAKLDEVYKLWEGLGYYRRAALLHKAAQI
                     VVTQYQGRIPDQYEFARSLPGFGDYTIGAVLSIAYQKKLPAIDGNVIRVMSRLYRIES
                     EVHLSATKRQIESLVQSMIPDENPGDFNQALMELGALICTPQSPQCTRCPVHRFCKSY
                     NELPNPNILPNKKKKTIKPHMHIAAGIVFKNKRILAAKRPSDVILGNLWEFPGGKKES
                     GESLLKTCERELQNKIGIRAKIGKCFLVHKHAYSHYTITLHFYKCDYIGGQPIKRKHS
                     ALRWVYPKKALELAWATSHRKALEKLLKDAIY"
     gene            complement(26365..28272)
                     /locus_tag="PK937_02715"
     CDS             complement(26365..28272)
                     /locus_tag="PK937_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNH31516.1"
                     /translation="MADTTEVEYIIDQNQTQVNLSSAPSLQVRTYIPHKIMRSLYYFV
                     LASGLILFIVSVNNLYLNNRIPWNTMVHDVSVGGMTVSQAREALTVYVDEFKNRDVRL
                     LTEEGEFLTKNTEFQPTFDIEKAIVTAFERGHNPDPLKNVQTRTASMFFSVRIPLESW
                     YDENRFVRTIINKIPKLKNNQPIDATVAWADRQFKAVAGRGGLGFDEEKAKKSFSENI
                     RSFASKDIPVDILSKEPDVTIAATTKAIRRAYAAYGKNVRMYYTYDGYNYDSWAFAIS
                     DRREFIEFRKVWEGNEFVLYPVLNAEMLREELNRRVAPYMYRAKEDVTIRDDNGKIVV
                     EGVARDGYYLDIPRSIININESVMKGILDTVGNIRADLVVAHLTGGISNPDNPFGITD
                     VLATGVTDYFGSPENRKFNINHAAKNFQNIVVKPGEDFSFLKYLGEVDSLSGYLKELV
                     IVNGDSSEPNWGGGVCQVSSTLFRTVFFAGLNVKKRTNHSYAVKYYEPLGLDATIFIP
                     APDLVFENDTKNTIIIQNLVDLKRTKMYFKLFGKKDGRKVEYEGPIDQGTVGEKNEHY
                     RVMWTRSVELPDGTKRTDKFGSTYKNKDLVKKHKPDSTLHNSADSTGLRNGVVFGDSA
                     HTGATTSNTPN"
     gene            28445..30004
                     /locus_tag="PK937_02720"
     CDS             28445..30004
                     /locus_tag="PK937_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH31517.1"
                     /translation="MRVSLEKKILVTAAASILFITGIAIFGFHYFSSRLSEQLIDNKI
                     GQALASGRQIAENLIEDERRIFSENARAFSQRPEFTDFIIRLQGQKLTSDQFQAFIGL
                     PLFTQLRVDGVFLYDEKGRCLFRHLSILDKRLSSVFPDSLKTDERFARSTSAYDSINR
                     SLVYKTTLALPSQQQGYLITARMVEKEFAKKYERIVAANNQYALLLRDADNMRFTHLW
                     IFITAVLAPAFIFILLLWFFIGQMFKPFQALSRQASNIAKGQMDYSLQIDTRDEFGAL
                     AAALNQMVQNLRDEQQRILQLEKTATWREMAQRMAHEIKNPLTPIQLTIQQIKDSYAG
                     NDEKYRKLLTECTTIIEEEIETLRNLTKEFSDFARMPVVVLKPSSLNKLIEDIILMYA
                     AIPFEKKLDDRLPFIPLDTEAFKRVLINLIDNALAAVSNKQDRLIRIATIDVNTHVQL
                     IIADNGYGIPKSNLERIFEPHFSTKTTSMGLGLAIVKKIIEEHQGTISVDSIENLGTT
                     FTITLPKENPS"
     gene            30017..30427
                     /locus_tag="PK937_02725"
     CDS             30017..30427
                     /locus_tag="PK937_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNH31518.1"
                     /translation="MAYWIVKSEPSVYSFEQFQKDKTTVWDGVKNPAALINLRSMKKG
                     DTVLFYHSNDGKCLVGTAVVNKEAYPDPKSKNPKLVVVELSVGKALAKPVTLAEIKSN
                     TKLAQLELVRISRLSVSKVPDTLWKELMKMAGEK"
     gene            complement(30428..31321)
                     /locus_tag="PK937_02730"
     CDS             complement(30428..31321)
                     /locus_tag="PK937_02730"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNH31519.1"
                     /translation="MKSNAPVQQTDFKNLKLMRRGKVRDVYDLGDKLLIVVTDRISAF
                     DVIMKDTIPQKGVVLNTMASFWFDQTRHIIRNHVITTDVEKYPEPCKAYRDELQGRSM
                     LVEKAKSLPVECIVRGYISGSGWNEYQKTGAVCGVVLPQGLKESDKLPEPIFTPTTKA
                     EQGHDQNITFDEMCKTISKDVAEQVRATSIKIYQFAQTLAASKGIIIADTKMEFGIKD
                     GELILIDELLSPDSSRFWPADQYKPGDKQPSYDKQFLRDYLLAIKWDNNTAAPILPED
                     IIKKTGEKYREALRLITGINL"
     gene            complement(31318..31803)
                     /locus_tag="PK937_02735"
     CDS             complement(31318..31803)
                     /locus_tag="PK937_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31520.1"
                     /translation="MTLQALDIESLKLLSENRNKFAQHLGVNVPEQWPALDLIEALPF
                     FIGLIEQDPSEYMWLVWITVETKSKNIVGGIGFKGAPDIQGTVEVGYDTAEMYRGFGL
                     ATEALQGITKWAFEQSEVRRVIAETDQSNFQSHRVLEKAGYLKSGENETMFFWERLRS
                     L"
     gene            complement(31821..34865)
                     /gene="secA"
                     /locus_tag="PK937_02740"
     CDS             complement(31821..34865)
                     /gene="secA"
                     /locus_tag="PK937_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNH31521.1"
                     /translation="MIQSLLSKIFGNKHERDVKKLYPIVDAINQNFQQFDTLSDDELR
                     AKTNEFRDRIRENTKEEIAAVQAIKETIRTNTDPNRSKTLVEELKVAEKTLREAEQYI
                     LDEILPEAFACVKQACKRLVGQKYMVCGREVKWDMVPYDVQMIGGIVLHEGKISEMST
                     GEGKTLVAVAPVYLNALAGHGVHLVTVNDYLALRDKEWMGKVYEFLGLTIGVILNDMN
                     PTQRRVAYGCDITYGTNNEFGFDYLRDNMATSPDDVVQIRGHHYAIVDEVDSVLIDEA
                     RTPLIISGAVDTPTNQKYEESNPGVRRLVDLQTRLVSQLVADADKLITEGKNKEAGIK
                     LLMAQRGAPKNKRLMKAMTEEGVAQLIQSVESEYLRDKKMHEIDEELFFAIDEKNHGI
                     DLTEKGRNALSPSNPEMFVIPDLSEEIIKIDGNKELTDHQKAIKKEELGTLYAERSDR
                     IHSIEKLLTAYSLYEKDVEYVVQDGKVQIVDEFTGRILHGRRYSDGLHQAIEAKENVK
                     VEKDTQTLATITLQNYFRLYSKLAGMTGTASTESTEFYEIYKLDVTAIPTNKPIQRED
                     FEDMIYRTKREKYNAVIELIRENHKAGKPTLVGTTSVEVSETLSRMLSRTGIKHEVLN
                     AKQNQREAEIISNAGQKSAVTIATNMAGRGTDIKLGSGVVEAGGLQIIGTERHESRRI
                     DLQLRGRSGRQGDPGSSVFFISLEDDLMRLFGSDRIARVMDKLGAEEGEVISHRWITK
                     AVERAQKRVEFRNFSIRKRLIEYDDVMNQQREIIYSRRRRALMGENIKVEFLDVLDEF
                     VEMLNDRFVKNQPHPEDWDLVGLTETLLRSCGIAVAQDEFYKLNAEQFSVHIRGMLME
                     RYQQREEVWSSENMRRIERIAVLKTIDERWKEHLREMEEFKEGIHLRGYGQKDPLIEY
                     KTEGFEMFMQLLDRITSEIVEFVFRAEPAAEMEQQMIRRARAMKAVHASSEGMGFSGS
                     GEEQAERPVQSTTTPIRAEQKVGRNDPCPCGSGKKYKQCHGK"
     gene            complement(34896..35597)
                     /locus_tag="PK937_02745"
     CDS             complement(34896..35597)
                     /locus_tag="PK937_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNH31522.1"
                     /translation="MRIAIISDIHGNIEALRQALDIIGELGTDEIVCLGDIVGYGPDP
                     EPCVDLIRKHCKTVIMGNHDNAVIDMSATENFNPVAKQAVWWTHQQLSSESLSFLQGL
                     PYRNQRQDLLFVHASPMNPAKWTYLFTINDALPEFDAFQERICFVGHTHSPRVFCNSP
                     HATAITREFQFIINVGSVGQPRDGDARLSFGLFDTEKWDYKNIRSEYDVQTTARKTRE
                     RGLPVILAERLLRGR"
     gene            complement(35599..36684)
                     /locus_tag="PK937_02750"
     CDS             complement(35599..36684)
                     /locus_tag="PK937_02750"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNH31523.1"
                     /translation="MRIGISTGGGDCPGLNAVIRGVTVSAINNYGWEVIGIQDGFEGL
                     LDTSKTQVLKLKDVEGIIGLGGTILGTTNHGNPFKYPVKKNGTTEIKDLSDVVLANIK
                     KLELDALIVVGGDGTQTISRDLWRKGAPIVGIPKTIDNDLLSTDVTIGYNTAVTIAVD
                     ALDKLHTTAKSHHRAMVVEVMGRYTGWIALEAGISGAVDVILIPEIPFEISKVCAHLQ
                     KRIEQGKNYSIIMVAEGAMPVGGKTMILETAEQNVGGAERLGGIGNWVSAQIEKESGV
                     ESRCVVLGHIQRGGPPSHIDRLFASRMGAYAVELAQQKMFGYMASARNKEIIHVSIEN
                     AVEKIRYINPDSETVKSARQIGISFGN"
     gene            complement(36793..>37560)
                     /locus_tag="PK937_02755"
     CDS             complement(36793..>37560)
                     /locus_tag="PK937_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31524.1"
                     /translation="GAEIANVCNEASLLASRKDKERIEMSDFQEAIERVIGGLEKKNK
                     IISPRERVIVAYHESGHAVVGHYLENADPVHKVSIVPRGIGALGYTLQTPLEDRYLLS
                     KDELIERMCSLLGGRAAEDIVLGKVSTGASNDLERVTEIANRMVTMYGMSDKLGNLSF
                     TEQGRENFLGGVNMKPYSEETAKLIDSEVKSIVDMAYEKTKQLLIDRREELEALTQRL
                     LEKEVLDKKQIEEILGEKPLPENNGHVPKEKTIATPE"
     assembly_gap    37561..37660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(37661..37887)
                     /locus_tag="PK937_02760"
                     /pseudo
     CDS             complement(37661..37887)
                     /locus_tag="PK937_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081278.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(38113..38382)
                     /locus_tag="PK937_02765"
                     /pseudo
     CDS             complement(38113..38382)
                     /locus_tag="PK937_02765"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38464..40914)
                     /gene="lon"
                     /locus_tag="PK937_02770"
     CDS             complement(38464..40914)
                     /gene="lon"
                     /locus_tag="PK937_02770"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNH31525.1"
                     /translation="MTKTLEEQISTKEKKKRGGQVPDSLPIIPLRNTVLFPQQIMPLS
                     IGRDKTLRLVSEFADANRLIGVVAQKESAIENPSQTEMYEYGVAATIVRVFDMPDGSK
                     SVIVQGVQRIRINDFIQEDPYWIANVTELGDIPAPASDLEIDALMNNIKNIFQKMSGL
                     APYITAEQTTMILNIQQPGRLSDAAVAALNIPTDEKQGILQILDVKIRLEQTHIMLTK
                     VLQTLELGNKIQSDVQDEISKTQREYYLREQLKAIQRELGEVETGNPEIAELREKVEK
                     ANMPKEALEVAKKELERLGRMSPMAAEYTVSRTYIDWLIDIPWNIFTEDNLNIKEAHE
                     RLDSDHFGLDKVKKRILEYLAVRKLKNDMRGPILCFVGPPGVGKTSLGRSIANALGRK
                     FVRVALGGIRDEAEVRGHRRTYIGALPGRVLQGIKKAGSSNPVFMLDEIDKVGMDFRG
                     DPSSALLEVLDPEQNNSFSDHYLEVPFDLSKVLFIATANLADPIIPALRDRMEVIEIP
                     GYTEEEKIKIAEKFIIPKQTKEHGLKENQIRFTKDAVRMIVREYTREAGLRNLEREMA
                     TVCRGVAREVAENKITKKTIDGKNLHEYLGRAKFFLDASERIKKPGIVTGLAWTAAGG
                     DILFIEASKMRGKGALTLTGQLGDVMKESAVAALSQIKSQAEALGITEDFDKIDIHIH
                     VPAGAIPKDGPSAGITMYTALYSLLTDRLVRNDVAMTGEITLRGTVLPIGGVKEKILA
                     AHRAGLRTIILPDKNEKDLDEVPAQIRKEMTFIPVNEVSKVIENALRPDKRAVHSSHG
                     NGKIKPKKTLRKKIAYSK"
     gene            complement(40992..42515)
                     /locus_tag="PK937_02775"
     CDS             complement(40992..42515)
                     /locus_tag="PK937_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNH31526.1"
                     /translation="MVSTRSSKLIAGISLIIIGIVAGVILTANFNFTHKGKASAESEQ
                     RVKLEETMATMKDFSEGFSAVAEYVTPSVVTVETEKTVRVDSRQFNPFGDFFGDNDPF
                     QQFFGAPRGGQPQEQKLSGLGSGVIVSEDGYIITNNHVIDNTDKIKVSLSNEKTYDAK
                     LIGTDPRTDIAILKIDEKGLPAVKIADSDKIKVGQWAVAIGNPFSKQLSHTVTAGIVS
                     GIARSSINSDTDVDFLQTDAAINPGNSGGALVNMSGELIGINAAILSKSGGYEGIGFA
                     IPSNVAKSVMDQLIKNGKVVRGYVGVQMQDISEEMAKALSLKEPKGAVVAAIMEDSPA
                     EKAGLQQGDVITKVNGKSVENSAEIRKNIVNKNPGTSVDLGVIRDGKDMTIKVVLGEA
                     PSEQPVAASKEESTTAKKNAERLGMGIANLSRDLAQKYGFNAKEGGVVITSIDQNGPA
                     FRGGLREGDVIKRVGRTDIKSTKEFSEAVNKISKGDTALFLVNRKGTSMFIAFNIPN"
     gene            complement(42625..43491)
                     /locus_tag="PK937_02780"
     CDS             complement(42625..43491)
                     /locus_tag="PK937_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNH31527.1"
                     /translation="MKDYYKILGVSESASSEDIKTAYKNLAKKYHPDRNPGNKQSEEK
                     FKEMSEAYNILSDVKKRKEYDTMRRFGGQGSGFSGSDNFDLNDFMHNFGFSGQNASFR
                     SSGGFSDLFDEMFFGKGGAHASEANQRVELTITFEKSIHGGEVDFTLRQHGNQNLRVK
                     IPAGIQDGEVLRIQNPNIGSINLTIRVQPDKAFTRKGIDIYADVQANLAQLMFGSSVR
                     VHTVYGNAIDVKIQPGTQPGTILKLSGFGVKTSRNHGDMYVTIKTKIPGHLNKHQKEL
                     FENFAKSLDLKW"
     gene            complement(43501..44388)
                     /gene="htpX"
                     /locus_tag="PK937_02785"
     CDS             complement(43501..44388)
                     /gene="htpX"
                     /locus_tag="PK937_02785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNH31528.1"
                     /translation="MKILNTFKTMFFLTTLVLLFGFIGMMIGGKSGMIIALILAGLMN
                     FVTYWFSDKIVLSMYGAKEVQYADQPKLYSMVQNLAQRAGLPMPRVYIIPTDTPNAFA
                     TGRDPQHGAVAVTDGIMARLSDDEMEGVIAHELGHIKNRDILISTIVATIAGAISSLA
                     SMAQWAAIFGGFNRSDDEDNGGGGNIVSAMIIAIVAPIAAMLIQLAISRSREFLADQA
                     GAEISGKPMGLANALIKLEQTNLRLPMDANQATAHLFIVSPLSGKGFVNLFRTHPTTE
                     ERVAKLKQWEEQQYGLITR"
     gene            complement(44505..44723)
                     /locus_tag="PK937_02790"
     CDS             complement(44505..44723)
                     /locus_tag="PK937_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH31529.1"
                     /translation="MPTYDYKCKNGHVFEEFHGINDSPETVCPQCGQPAVKEIGAGSG
                     LMFKGSGFYITDYKKNSGSDSGSKSGKE"
     gene            complement(44771..>46425)
                     /gene="dnaK"
                     /locus_tag="PK937_02795"
     CDS             complement(44771..>46425)
                     /gene="dnaK"
                     /locus_tag="PK937_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNH31530.1"
                     /translation="VPYEVTAGDNNTARVKVADNMYSPPEISAMVLQKMKQTAEDYLG
                     QTVTDAVITVPAYFNDAQRQATKDAGKIAGLNVLRILNEPTAAALAYGLDKKKNEKIA
                     VYDLGGGTFDISILEIGDGVFEVLATNGDTHLGGDDFDDLIMNYLADEFKKNEGIDLR
                     KDPMALQRLKDAAEKAKIELSSSQQTQINLPYITAVDSVPKHLDITLTRSKFEQICDT
                     LIQRTVKPVEQAMKDAGLQYAQIDEVILVGGSTRIPKIQQLVQDLFKREPHKGVNPDE
                     VVAVGAAIQGAVLAGEVKDVLLLDVTPLSLGIETLGGVFTAMIPRNTTIPTKKSEIFS
                     TAADSQTAVTIRVGQGERQMFADNRELGRFELMGIPPAPRGVPQIEVTFDIDANGILH
                     VTAKDKATNKEQQIRIESSSGLNKDEIDRMVNQAQEHAEEDKKRREKIDTKNQAESLA
                     YQTEKNIKEFGDKVDADSKSRLEGEMQKLKDAIATDNTDTMKSAMESLSAIWNEVSSK
                     MYQAGAGAQGATGAGPTDGGAQQQSGQKVEEADYEVVDENKK"
BASE COUNT        12336 a        10785 c         9534 g        13670 t
ORIGIN      
        1 ccgccatatt gaatgatcat cttatctgat attgtttttc caaatgactc ccgcgctaag
       61 tcgcgtataa acttatgtac cgcttcggct tcggcaaccg tagcattttt gccagttcca
      121 atagcccaaa tcggttcgta tgcaataatt attttggcgg ctttatctga atccagattt
      181 ttcattgcac cgaggtattg ctgacgaata actgattcgg tatgtccttc ctgacgctgt
      241 tcaagcgtct caccgacgca tacaatcggc gtcagtaatt cctgcaaagc aaattcaatt
      301 ttatgattca ccgtcgtatc ggtttcaaaa aaatgttgcc gtctttccga atgcccgatg
      361 atcacatatt cgcagccgga ttctttgatc atttcggcag aaatctcacc ggtataagct
      421 ccgtatttat gcaaatacat attctgcgca ccgactttga ttttagtgtc tttcaaaaca
      481 tcacgcaccg cagcaatact gatggaaggg gggcaaacag ccatctccgt tttacgaata
      541 tcggttaatt tcagtttcag cgatttggct aagtgcaggg ccgcattcgg gcctttatgc
      601 attttccaat ttccgacgat taccttttta cgcatgggtc aaagcttcca caccgggcaa
      661 aatttttcct tcgagaaatt cgagagacgc accgccgccc gtggacacgt gcgtgacttg
      721 tttttcaaga cccaattccg ttatcgcggc cgccgaatca ccaccgccga caatggttac
      781 cccgcctttt tgtgtcgctt cgaccaaact ttcggctatg gctttagtgc cttttgcaaa
      841 attgggcatt tcaaaaacac ccatcggccc attccacacg accgttcctg cggaattgat
      901 ctcgttacga aacatatcaa tcgtaaccgg accgatgtct aaacccatcc agccggacgg
      961 tatttgatcc atcgaaacgg ttttaatttc ggcttcattt ttaaactccg tagcgacaac
     1021 agcatcaacc ggaaacaaaa cgcgtgtagt accgcttttc gctctttcga ttatagactt
     1081 tgctaaatca gctttttcgg cctcaaaaag cgaattacct atttcatacc ccatcacttt
     1141 ggcaaaagta aacgccatac cgccgccgat aatgatggta tccacttttt tcattagatt
     1201 ttcgattaca tctattttac cggaaatttt ggcgccgccg atgatggcaa caaaaggccg
     1261 tttggggttt tgaaccgcat tgccgagata acgcaattct ttttccataa ggtaacccgc
     1321 gatattttgt ttgaaatacc gcgtaacgcc ctcggtagat gcatgtgcgc ggtgtgctgt
     1381 gccaaaagca tcattgacat atatgtcgcc aagcttggcc agtttttcag caaaagcgat
     1441 atcgttggct tcttcttcat tataatatcg gagattttca agcaacaata cttgcccttt
     1501 ggataaaccc gccgcttgtt tttcgacggt atcgccgata caatcttccg ccattattac
     1561 gggttgtccc aaaagttctg aaagcctcac agcgcaaggt tttagagaca tcgcaggatt
     1621 ttttttccct tttggccgcc ccaaatgact catcaaaacc ggaataccgc cgtcgctcaa
     1681 tatcttcttt attgtcggca gagattcacg aatgcgtgta tcgtccgtaa tgcggagtgt
     1741 gtcgtccaac ggaacattaa aatcaacccg tacaagtacc ttttttccgg cgacatggac
     1801 ttgatctatc gtaagcttat tcatcatcgt ttcgtccgtt taggctttgg ttattttcat
     1861 caagagatca acgcaacgat tggaatagcc ccattcgtta tcgtaccaag aaacgacttt
     1921 aaaaaatcgt ttttcacctt tgaggttgtt ctgcgtggtt gctaatgaat cataaatgga
     1981 tgagcgttca tcatgaataa aatctgtcga gacaagctct tcgttgcaat agccaagtat
     2041 gttttttaag tacgtttccg aagctttttt cattaatccg tcaatttctt caatggacgt
     2101 atcgcgcgtt gcgcgaaaag taagatccac gacggataca tcggccgtcg gtacgcggaa
     2161 agacataccg gtaagttttc ctttggtcgc aggcaacact tcccctacgg ctttagcggc
     2221 accggtagtc gaaggaatga tatttattgc cgcagcacgg ccaccgcgcc agtctttttt
     2281 agaagggccg tctacggttt tttgcgtcgc ggtataactg tgaatcgtcg tcatcagacc
     2341 cgtttcgata ccgatacctt ctttgagtaa aacgtgtacc aacggtgcaa ggcagttcgt
     2401 cgtacaggat gcattggaaa tgatgtgatg tttggaagca tcgtattctt gctcattgac
     2461 acccataacg atggttttga catccccttt accgggtgct gaaataatta ctttcttagc
     2521 tccggccgtt aaatggcctt tcgctttttc cgaatccgta aacaaacctg tggattcaat
     2581 cacataatcc acacccagat ctttccaagg cagttgcgac ggatctttgg tcgctgcaac
     2641 acactgtacc cgatcaccgt tgacaatgag aatgtcattg tgatccaaat ccggtttact
     2701 tttggcaaca ttgatagtgc ctttaaattt tccgtgaata gaatcatact tgagttggta
     2761 ggcgaaataa tccgcatctg tactgacatc tactacagca actacgtcta tttctttacc
     2821 caaaaggcct ttatctacaa gggcattaaa tactaaacgc ccgatgcgac cgaatccgtt
     2881 aataccgact ttgatagcca taatcctctc ccgaaaagcg ttgaaaatta tgaagttgtt
     2941 aaactggggc gaatatagga tttgtatgat ggaaagtcaa ccgaaaataa ggattgcgac
     3001 gctgtcatat caacccttcg gcaatcattt gcagtttttg catcgtcgcc agattccgag
     3061 ttgtggcttg ggtgtctaat ttcttttcaa taaagttgtt cgagaatttg gagtttccgt
     3121 agccttttcg acacagaata taaaccgttt tcccgttcaa ttgaaattca tcctttgtat
     3181 ccgataatgc gttcagggat tgtgctaaat cgggttttgg ttttatagct aataacgtca
     3241 cgtacacgcg ctcatcaggc ctgagttttt tctttgcata aggattattt tcatttacga
     3301 ttacccattc gttcaaacta cgcgcaataa cagggacaat atagcccaag gaaacggcta
     3361 attgttcccc cacttttttg ctcaatttct cggactcttg tttggcactc tcaaagacta
     3421 catttccgct ttgaatatac gtttgtacat tcttataacc gtacgaaaca aaataacttt
     3481 tcaaatcatt cattttgatc agtttttgtc ccgacacatt gataccacga agcaatgcga
     3541 tgtatgttga cataatggct tttgctcagt tttagaataa attccatgaa gcgttttttt
     3601 cggatgatgt tgccgcatca cgagagcgaa tcgtctgagc taatgaaacc aacgtactgc
     3661 gtaatgcgtt ggaaatttct ttatgcgtat aaatttcatc gtcggacagt ttttggcctg
     3721 ctgatggcaa tactttggat gctacgggat caatatctgc cgtcatcacc cgtagtgttt
     3781 cgatgattga agcgtatgca tacgtggcat gcggtgcggc atttattaca gcgaccggtt
     3841 tgtataccaa ttcaccgctt cccacgaccc aatccaatgc gtttttcatc gctccggtta
     3901 caccgtgtgc atattctgga ctagcaataa ctatcccgtc atgggaagct aattgattac
     3961 ggaaatgtaa aactgaaggg aacgcttgat cttcaatgtc tggattaaat agcggcaatt
     4021 cgccgagacc gtaaaacaac tctacagaca cattttcagg cgctagtttt atcatagccc
     4081 gtagtaacgc gctgttatac gaattttttc taaggctccc tgaaatggct aatattttta
     4141 tcatatgctt ttcccatcgc gctctttggc tactacatcc attgccgtag ataaaacgcg
     4201 atcaaagatt tttcgcatct ggcgtaatga tatgacaaca aaaaccgcaa cgcaccctaa
     4261 aatcaaaatt aaattgataa ccgacccgat atataacgcg ttagcacgtt tgacttcatc
     4321 gtcggcggct ctgcgatttt cgtgcacaag cgccgaaagt gcagacgaat actcattgaa
     4381 taaaacttcc gaacgcgcac gaatcaactg ttttgcatct tcgtttttgt tgagtttgga
     4441 cagttgaagc atatcgatat ggtgcttcat atactcagac catctattca aaaaatcctc
     4501 atacagagtt ttttctacgg acgtcgtaat cagaggttca tacagtttca tctccgagtt
     4561 aatcaaatcg gctatgcgtt gcatagctga ttcatacgta cgcatctgat cgtctgaagt
     4621 ggacaaaata tgctggagct ccataatccg aaaatcggac gtgagtgtgt ttaagttacc
     4681 gacatgcatt acgctgggca accaattctc gctaatctgt ttggatttgt catgaatacg
     4741 atgaataaaa aaaattaccg ccgtaagcgc cgcaatcaat aacaatgaaa gtatctgaaa
     4801 tagctgtttg ggtgaacgca tgctgattct ccgaaatatc aacgttttca tattccattt
     4861 acataaaata caaactttac gtaaaaatct ctacatgaat aatgattgtt acattcgtca
     4921 aaaaacattg gatgaatgtc tttttttttc tatcttgcgc cgcacgaatt gcgttaagta
     4981 tgttatcaaa aatccaataa gtaacactat gtcagaagac atcaaagaag aatgtggtgt
     5041 gtttggtatt tacggccatc cagaagcatc acgactgact tattttggct tgtattccct
     5101 ccaacatcgc ggacaagaat ccaccggcat cgtatcggcc gatggtgata gaacctatcg
     5161 ccatgtcggt atgggattag tcagcgacgt attcgctgat gataaaattt ttgatcgctt
     5221 acctggtcat tcggccatcg gtcataatcg gtattcaacg accggcggaa gttttatgca
     5281 aaacgtgcaa cctttgacgg ctattattag caccggtccg atatccgctg cccataacgg
     5341 aaacctgact aactatcaaa gtcttcgccg cgaactccaa aataacggag ccatccttca
     5401 aggaaacaac gacaccgaag tcattcttca tctcgctgca cgttccggta aaaccactgt
     5461 cgaagaacaa attatcgccg cattgcatca gattcggggc gcatattcgc tacttctgat
     5521 aactaaagac aaactcattg ctgcacgtga tccgtggggt atccgtccgc tctgtatcgg
     5581 aaaattggac caagctaccg taatagcaag tgagtcctgt gcgttggaca tcatcggcgc
     5641 cgaatatgtg cgtgacgtaa agccgggcga atttatcgtc gtggatcatg accgtatgca
     5701 aacacgaatg atttttgacc cgctacccac accggcgcat tgtatttttg aatttgttta
     5761 tttttctcgt cctgacagta aaatttttgg cgataatgtc gacaaagcac ggcgtaaact
     5821 cggaaaacgt ctcgctctgg aacacagcgt ggacgcggat atcgtgatca gtgtaccgga
     5881 ctccagcaat accgcagccg tcggttattc acgccgaacg ggattgaaat ttgaactcgg
     5941 tcttattcgt aaccattatg taggccgcac ctttattcat cccgtacagt ctatgcgcga
     6001 tctgaaagtg cgattaaagt ttaatgcggt agaaggcgtt ctgcgtgatc gccgtgttgt
     6061 cgtcattgac gattcgatcg tgcgcggcac tacgctcaaa gctttggtta cgatgatacg
     6121 taaagccggg gccaaagaag tgcacattcg tatcagttcc ccgcccgtca cttcaccatg
     6181 ttactatggc atggatttcc cgaccaaaga agaattgatc gccaatacca tgaataaaga
     6241 tacactctgc aaaagtatcg gcgccgattc gttggagtat ctttctcttg acggtatgtt
     6301 ggagtgtgtg cccaacggcc cacacaacta ctgcacagct tgctttagcg ggaaatatcc
     6361 cgtacccatc gaacccgata cacaaatcac ccaatgtgat taaccgcaag ccttatatat
     6421 tcgcagttgg cctttatgat ttttctcatg gtattctgcg cttggcgact gtaacctttc
     6481 tttgatttac gtttgacctg attacttcag ataggctcgt ggttgcttgt tttgagcgcc
     6541 tatctatatt caatcaatca tttctaaata attaaattct ctcaccgaac ttcaagcgag
     6601 acacgtatga gcgaacattc ttctcaaggc gtcaaaggtt taccctccaa tgcctacacg
     6661 gaattaaagc ccggcgaaac ctacaccccc attatgtcac cgcagcaggt gtatcccgaa
     6721 gtaaatttca ggtctgtttt ttggggactg atcatggcga tgttgttttc tgcggccgcc
     6781 gcatatttag gactcaaaat cggtcaggtt tttgaagccg ccatccctat cgcgatcatc
     6841 gccgtaggac tttccgccgc ttcgaaacgc aaaaatgcac tgggtgaaaa tgtaatcatt
     6901 caatccatcg gagccaatag cggcgcggtg gtagcaggcg ccatatttac aatacctgcg
     6961 ctttacattc tcaacctgca agctgacttt ttcaaagtat tcctagcatc gcttttcggt
     7021 gcttttctag gaaccctatt cctgattcca ttccgtaaat atttcgtagc cgacatgcac
     7081 ggtaagttcc cctttccgga agcaacggcc acaaccgaag ttcttgtggc cggcgaaaaa
     7141 ggcggcaatc aagctaaagt attggtatta tcaggcatca taggcggaat ttacgatttt
     7201 atcgtggcta cttttggttg gtggagcgaa gttttttcaa cccgcgcatt gccatggggc
     7261 atggaattag ccgataaagc caaacttgtt tttaaagtga atatcggtgc cgccgtaacc
     7321 ggacttggtt ttattatcgg tctgcgttat tcattgatca ttgcttgcgg ctcattcgtc
     7381 tcgtggtatc ttctgatacc gaccgtatat tacataggcg caggccttac gacaccgctc
     7441 ggtgccaatg caaccaaact gatcggtgct atgacggcgg aggaaatttt tagaaattac
     7501 gttcgtcata tcggcatcgg cggtatcgca atggccggca tcattggcat tattcgttca
     7561 tccggtatca tcaagtcagc tttcatctta ggattcaaag aaatcggcgg taacaaaggc
     7621 gacaacgcta atgccttgcg cacccaacgt gatctgggca tgaaaatcgt cggcatcggt
     7681 attttggttg tagccgttat gattttcata ttttttcaaa tgggcgttgt acaaaatctg
     7741 acccacgcac tgatcggact cgctgtggta ctcattattt cgtttttgtt tacgaccgtt
     7801 gcggctaatg cgatcgctat tgtcggaaca aatcccgttt ccggtatgac cttaatgacg
     7861 cttatcatcg cgtctgttgt attagtttca tccggcctta caggtgaagt cggtatgctc
     7921 tcggcactga tcatcggcgg cgtagtttgt acggctttat cgtgtgccgg tgcttttatt
     7981 acggacctta aaatcggata ttggctcggt tcctccccgt acaaacaaga gacatggaaa
     8041 tttgcgggtg cattagtagc gtcgcttact gtaggatggg taatcatgct tctcaatgaa
     8101 acctacggat ttaccggacc tgatgcatta gttgcaccac aagccaatgc catggctgcc
     8161 gtcatacagc cgctgatgac cggtcaaccg gcaccgtgga tgctgtatgt cggaggtgcg
     8221 tttatggcga tagtactggc atggttaggc gtgccggcac tcgcttttgc cctagggatg
     8281 tatattccct tagaactcaa tacacctttg cttttcggcg gcctcgttgc gcactgggtt
     8341 tcaacccgtt ccaccgacga aaaacttaac aacgcgcgtc gcgaacgcgg cacgctcatc
     8401 gcttcgggct tcattgccgg cggcgcactc atgggcgtca tcagcgcgat cttacgctat
     8461 gcgggatata actggtttaa tgccgaatgg gctgaatcac acggcggtga attattagct
     8521 ggtttgatgt ttgtgggaat tctgatctat atgtattggg actcgatgcg tggtaaaccg
     8581 gaagaagaat agagattata aaaatatcac aaaaagaaaa gccctcgttg atgagggctt
     8641 ttcttttttt tgtcggaatg agaggatttg aacctccgac ccctcccacc ccaagggagt
     8701 gcgctatccg ggctgcgcca cattccgatc cttgttattc aatcaacggc ggcaatatat
     8761 actaccgatt acaaattctc aaggttttta atagcttttt gtaaattttc ttctgacgta
     8821 gcaaatgagg cgcggatata accttcgccc gagattccaa aagcacttcc gggtacagcg
     8881 gctatattgc atttatcaag tatatattcg catgcggcga cagaattcat acctaacttt
     8941 ttcacattga tccatgcata aaaagcccct tccggcctaa agcatggcaa accatattta
     9001 gtaaatccct gcacgacgat ttcgcgacgt acagcataag ctgacgtcat gacttgaata
     9061 gcggattgat caccgttcaa ggcttcgaca cccgcccatt gactcaccga cgaagggcat
     9121 gttgctgaaa gctgttgcag tttattgatc tccgatatga tttgtttatg ggcggccaaa
     9181 tacgccagtc gccaacccgt catagagtat gccttggaaa aaccattgag tatgatcgta
     9241 cgatcgcgca tgcccggtaa accggctgca gggacaaaca tgtgattatc gtatagtatt
     9301 ttttcataaa tatcatccgt cagaagcatc agattgcgtt cattacatac agaacaaata
     9361 ctttcgattt ccctttttga aaggatctta ccggtaggat tagacggact attgatgatg
     9421 agtaatttgg ttttaggcgt caccgccgcg cgaaggcgat caatatctat acaaaaacta
     9481 tccgattcat acgtaggtac gccgatataa cggcctccgc tcattgcgat agaaccttcg
     9541 taacccaccc aacatggttc gatacatatc acttcatcgc ccggattgat tattgcctgg
     9601 atcacataca ccacggcttg ttttccgccg ggagtcacga tgatttctgt attcgcatca
     9661 tacgaaagtg aataatctcg ttgaagtttt tcggctatcg cctttcgtag aggaagtatc
     9721 ccgcggctgt tagtatactt agtattaccc gcttgtgctg cacgataggc ggcctcgcta
     9781 atatgcgccg gtgtcggaaa atcaggttct ccgatagtca gatctacgat atcagcgccc
     9841 gcatctttca gcgtctgtgc tttttcagca agccgaacgg tgggtgaacc tttcagcgtt
     9901 tggctcaagg atgaaaacgg atactgcatt atttacgctc cctatgttgc cctaatatcc
     9961 atcgaacggc ttcggtaact tcttcggcta tatgatctgg tttacacgct gttgatccgg
    10021 cattcatcgc atctattgtc ttttgtccat gtccagtttt taccagtacc gatcgtatga
    10081 cttcacgatt tgctaactca atatccgact tataatcccc gaccacaaac gaggcgctta
    10141 aatcaatatc aaatctcatt aacgcctgtc gaatcatgcc cgatgctggt ttgcggcaat
    10201 cgcatagttt agtgtattcg tgtatcgtgc cttccggatg atgtgggcaa taaaaaaagg
    10261 catccatgtg tgcctttttt tgttctaact gatccgccat aaaatcatga agacgattta
    10321 catcttgttc gctataaaaa ccacgcgcca ccccggcctg attggtaata acaaaaacca
    10381 aaaaacccgc ttcattaagc atatgcacgg cctctggcac tccagatatc cactcaaagt
    10441 cttcaggacg atacaggtaa tttttttcaa cattcagagt accgtcacga tcaaaaaaaa
    10501 cggcgggttt ggcctgcata cccatgctag tgataaccgt ttccgttagt ctgaattttt
    10561 agatctttga tcttaagctg tatagtcgtc cgaccttgcc aggtattctc atcaatctgg
    10621 tacaccaaat ccagcggatt atcacctacg ccgattttgt tgtaatagtg ccctagattg
    10681 aaacccacag cgtcaaacac ggcgccattt tggcgaactt tcattcggat atgacgctca
    10741 ttcatcagtt gcggataacc cacgatctgc aaagaacgcg atataaatat cggcctcata
    10801 ttagccgggc catgcggctt aaaaagatta agcaatgtca tcaaacggtt gttgatttca
    10861 tccaatgaaa tttcggcatc aatatggatt ttcgggatca gatcgttggg cggaagatga
    10921 atagcagcat attcattcag tcggcggcga aactcttcta tgttattgag gtctatggtc
    10981 aacccggccg catatttgtg acctccgtag ccgaccatga gatcttcaca cgacttaagc
    11041 gcctgaaaca catcaaaatt ttctacgctt cgtgccgaac ctttaccttt atcgtccgct
    11101 tcggcgatga ccacggctgg acgataataa cgttcaacca gtttgctggc cacgataccg
    11161 atcacacctt gatgccatcc ttttttatac accacaatga ctttatcatt ggcgaaatca
    11221 aactcttgat caacgatctc acaagcttcg agaaacatca cctcgttgag atcacggcga
    11281 ttgcgatttt cggattccaa aacactggca aattcgacag cttcggattc acttttagta
    11341 accaataatt ttactgctct attagcgtca cccaatcggc ctacggcatt gatacggggt
    11401 gctaatgaaa aaacaatctg tgcagttgaa ataggtttgc cgaacacatt ggcattcttg
    11461 accagctggc gaataccgac tttattggca tcgctgacta atttaagccc gtttttcact
    11521 aaggtgcgat tctcgcccgt aagcggtacg atgtctgcag cgctgccgat cgccaccaga
    11581 tcaagatgtt gcatcagcac atcttcgtct attttcaatg tctgcgtgat cgcctgtgct
    11641 aatttgaatg caacgcccac gccgcacagt tctttgtacg gataagggca cgattctttt
    11701 ttggggttga gaatagctag tgccgcgggt aattgatcgc cgggatgatg atggtcacaa
    11761 ataatcacat caatgccgta tttattggca agattgacct gatcaatagc ggttatacca
    11821 caatctaccg caataagtaa gtcaatcccc tgctcttgag catacttgat gcccgtcgga
    11881 gaaagtccat acccttcttt gacgcgatcc ggaatgtagt aggaaataaa tccgcggtga
    11941 atttctttga agaagagata caacatagag gtactcgtcg taccgtccac gtcataatcg
    12001 ccataaatca tgatggattc acggttattg atcgcgcggg ttacacgatc aaccgcaata
    12061 tccatattgt gcataagaaa aggatcattg agcggtgcgc tctctgcccg gaaaaaattt
    12121 tttgcctctt caaacgtacg aataccacga ttgaaaagga tgccggctat aagctccggt
    12181 acgttaattt cttccgctaa tttctttaca cgctccttgt catgatgatt gctcagtacc
    12241 cattgttgtt ccaacataag ggattgtaat ggtttgattt atatttagtt ttttaaacat
    12301 catagttgaa aaaaacccac ttttgaattc attagggccg aaagataata aataaaccct
    12361 tgataaaata tagtttatcg ctttgtaaaa tgcaaaatgt attttcaaac cttgcacggc
    12421 attgattttt ttggtatcgt acgcacaatc aaggaaaaaa ctatgttcaa acatcgcgtt
    12481 gcccgcaatc gttttttgta tgtcatactt ttaggtctga tcataacggg ttttggttat
    12541 ttaatgatca actatcaact caaaatgtat caaatggttt taatggcggc catgatcctt
    12601 atccccaatc gtttggcacg ctattattgg tttgattttt ttgcaggaaa acgcgaattg
    12661 cgtcgtaaac gacctgaaca agcaacaata cgttttcggc aattcctgga aactctgcgt
    12721 ggcgctcctt ggataaagtg gttgatgttt ttttcgtacg gcctgtattc tttcaaagta
    12781 gaagctgttg ctttagttta tctcgggcat gcatggcttt atttaaaaaa atatgatgat
    12841 gccgaaaaag cattccgcga tgcgctcttg gtggatgaca aatacgtcat ggctaagcga
    12901 ggcctcgccg ctgtttacat tttacgcgga caaaataatg aagcgcaaca atggcttgat
    12961 ttggcttgcc gttttggtca ccctaaaatc tcctttgaca aatttgaaga actcacgcgc
    13021 aaggaataca acgcatgatg cgcgtttgga agttaattgt tgattacggt cgcatgatca
    13081 agttttctca tacgattttt gcgctgccgt ttgctttaac gtcgtttgtc gtcagcggtc
    13141 gcgagttccc gctgaccttg tccaaatttt tttggatcgt cgttgcgatg gccggcgcac
    13201 gaagtgcggc gatgggattc aatcgtatcg tggacgcgcg ttatgatgca aaaaatccgc
    13261 gtacagtgaa tcgagaaatt cctcgcggcg ttatcacgca aaagcaagca tggatatttg
    13321 tggttatagc atcaaccgca ctggttttgg cctcctactt tctcaaccct ctatgtttta
    13381 tactatcgcc cgtcgccctt ttttttgttt tattttattc gcttaccaaa cgttttacgg
    13441 ctacagcaca tattttttta ggaattgcgc tgggaattgc tccgctggga gcgtggattg
    13501 ctatgtcggg ccaatgggat tggacagcat tcatgctagg ctgcgccgta ttaacttggg
    13561 tttccggatt tgatatcatc tacgcttgtc aagattacga atttgatcgc tctgaaggtt
    13621 tgttttcgat accgggcaaa tttggaattg ccaatgctct gtggatttcg cgcttcttgc
    13681 atgcgacagc catttttttt ctaacatacg tcggttctca ctgggaattg ggctggatat
    13741 atttcgcagg tgtgtgcata attgctactt tgctgatata cgaacaatcg ttagtacatg
    13801 ctcatgattt atccaaagta aatatcgctt tcatgaatat gaatggcatc atcagtatca
    13861 cctactttgt ttttacggta ttagatattt atctatagaa attaactctg ttaattttac
    13921 aattttacaa tatgatacaa tttattctga tcggattagg cggcgcactg ggcgcaatgg
    13981 caaggtatgg gcttcaatac gtcatacaac agcgttccgg tttgttgttt cctttgggta
    14041 ctttgattgt caatctcatc ggttgttttt ttattgggtt cattatggag atagctgaaa
    14101 tacgttcagc atttaatccg caaatccgat tatttatcac tgtgggattg ctcggcggtt
    14161 tcactacttt ttcagcgttt ggatacgaaa gcatacttat gatgcgtacg atggaatttt
    14221 atttttctat attttatata gtcggaagca ttgtcggcgg gcttgcactt gttatcttag
    14281 gtcaaacttt ggctagactt ttgtaattta tttacaacaa accatatact cctttctatt
    14341 tttcattgtt aatattctat ccgtttactc gctctattat atgttaatcc atgtcagatt
    14401 ggcattcaac ataacggttg tcaaatcgta aaactgcttg attttttatg gtacaaccaa
    14461 tatattcgcg acagaattac gtttttgttt ttgttaaact taatatgatt gaaacgatac
    14521 ggtgggcgca tcccgctgta cgcatcattg atcaaaccca attaccggtt tatgagactt
    14581 atctcacaat cgccgatctg gatgatctca ttgatgcaat taaaaaattg aaaattcgcg
    14641 gggctcctgc gttaggaatt gccggtgcat acggtttgtg tttaggactt tatcgttgta
    14701 ccacgtcaga taaagcaata cttcgcgacg aagctatcaa tacagccgcg atattgatcc
    14761 aaagtcgccc aaccgcagtt aatcttgcgt ggggcgtcca tcgtgcgcta aaggttttcg
    14821 aatctgttta ttccacggct tcgagcacag atatattgcg atcttctgtt cttaaggagg
    14881 cgcaccttat cctccaggaa gagttagatg cgtgtattgc gatgggaaaa ttaggctctg
    14941 aactcataga cgacggtatg caggttttga ctcattgcaa taccggcgga cttgcaaccg
    15001 gaggattcgg tacagcccta ggtgtactgg ttacggccca ccaaaacgga aaaagaatcc
    15061 atgtgtgggt tgatgaaacg cgaccacttt tacaaggcgc ccgattaacg tcatgggaat
    15121 taaaaaaaga aaatatacgc catacattga tttgcgataa tatggcggga caccttatgt
    15181 ccaaaggtcg cgttcaggct gtaatcgttg gttctgatcg aattgcacgt aatggtgatg
    15241 cggcaaataa aataggaact tatactttag cggtattgtg cgaaaaacat aaaataccat
    15301 tttatgtcgc cgctccgcat tcgacgattg acaaacaaat acctgacgga agcgctataa
    15361 ccattgaaga acgctcagct gacgaaataa ccatgggtat gggaaaacaa acagcgccgc
    15421 aaggtgtgca tgtatacaac cctgcatttg atgtaacacc ggccgaattg atttcggcta
    15481 taataactga gaaacaaata ttccgtggcc cggactattg tttcaattaa cacaaataat
    15541 attgtttcat tcacactaat gattggagat ttctaatgaa actggtattc aaatccgtag
    15601 ccgcgatgat tcttctgagt ctaattttag gtcaacaaat tttcgcgcag gaaaaaaaga
    15661 aaatcagcca ttccgcttct attcagggca tcaaacaacg tttaaaaaat gcttctaatg
    15721 aacccggtga tgcagacaaa tattatcagc aggcattgga aattctattg gtcaataacg
    15781 cgcaagttga taagaatgat aaagaaaaag aagaaacaca ctactatgcc gccgccgttt
    15841 atcatcgtat gaatgatatg atcaaagcat gggaatattg caataaaaca ttaaccttcg
    15901 gcaaaaaata cttcgaaaaa ggagaaaaag tttcaggcgg cattgagctt ttctccatca
    15961 aagatatgat gaatgatgtc aagctgaaaa catataacca aggcaatcag gcttaccgcg
    16021 ctgcagcggc cgccacatcc cctgactcac aaaaggtttt gtatcataaa accatttcaa
    16081 aattcaaatc attactagaa attgatccta ctgttaccat tacagccaat aacgtaccga
    16141 gttcgtatgc tttaggcgtc tatggaacga ttgctaccgt atatattcag cttcttaata
    16201 tggaaaaaga agaagccaat aaaaaaacca ttcgggaaga catcgtatca tatcttacca
    16261 agatgcatgc tcttgaccgc gctaatttct catttgtcac aagcattata agtctttaca
    16321 cacaagaaaa taatgaagaa aaaatgatgg cttggatgga tacggcctta gccatgaatg
    16381 caccggacag tcaagctgtt gcttatcaga cctccctcat cggcgataaa gcgcttatgc
    16441 ttgatcgtca aggtaaagcg gatgaagcca ttgccgtgta taccaaagca ttagaaaaaa
    16501 acccggaaaa tccggattta catttcaatc tcgggcgtct ctactataac cgtaaagatt
    16561 tcgataaagc caatactcaa ttccgtaaag tcaaaagcct gcgtaaagat gatccggttg
    16621 taaatttcca agtcgctgaa cagtcgtacc aatcctatca gagcaaacgc acggaaacga
    16681 ttgacaaaaa cggcggcgca cgcgccgata tgaacaaaat cactacgatg ctcaaatccg
    16741 acattgagat tgttcgcacg gatatagctg atgctatcgc atcattggaa gccagcttac
    16801 cgactacact tgaaatggct gaaacgaatt atcgtatcgg aaaaatgtat aattaccagg
    16861 cggaaattga agggcatctg tactatacgc tcgtcaacgc cgaaaaagtt aaaaaacaaa
    16921 aaccttattt tgaaaaagca attccctatt tggtaaaagc ctctgagctg cgcccttccc
    16981 atgcgaatac atggatcatg cttggtacag cctatgttaa tttgcagaaa aagcgtgaag
    17041 ccgaagccgc atttaaaaaa gctaacgaaa ttaaataatt tagataaaat tcagataggc
    17101 aaaacgtcgg cggtaaactt ggtatccgat ataccgccgg atgaacataa cgcatgataa
    17161 cacgatatac acgtccggag ctaggaaaaa tctggtcgga cgaaaataaa tttcaaaaat
    17221 ggcttgacgt ggagatagcc gtttgcgaag cgcaagcgga tttgggttat atacctggcg
    17281 atgcattagc cgctatcaaa tcccgtgcac gttttgacac ggcgcgcgtt ttggagatcg
    17341 aagccgaagt caagcatgac gtgatcgcat tccttacaaa cgtcgccgaa tatgtcggcg
    17401 aaccttcgcg ttttatacac cttggtatga catccaacga tgttttggat acttcccttt
    17461 cgttgacgat tcgagaagcc ggcaacatct tgcttcagga tatccaacag ctgcttgacg
    17521 tactccatcg ccgcgcattg gaattcaaga tgacaccttg tatgggacgg acgcacggtg
    17581 tgcatgctga accggttact ttcggtttaa aactggcact gtggtatgat gaaatgacgc
    17641 gcaatgccga gcgtttgaaa cgtgctgtcg aagcgatcag tgttgccaaa atatccggag
    17701 ccgtaggtac ctttgagcat ttggatccgc gtgttgaaga aaaaacagct tcaattctcg
    17761 gcttaaaacc gatcaatgca acacaagtcg tgccgcgtga cctccatgcc gattttcttg
    17821 taacgctggc cgtcatcggt gcatctttgg aaaaatttgc cactgaaatt cgcagtttac
    17881 agcgaacaga agttcttgaa gccgaagaat atttttctaa aggccaaaaa ggatcttcgg
    17941 caatgccgca caaacgtaat ccaatcacct gcgaacgtgt cgcaggattg gctcgcgttc
    18001 tccgcggtaa tgctcaagcg gcactggaga atgtggcgct gtggcatgaa agagatatta
    18061 cccactcttc cgtcgagcgt gtgatcatcc ctgacagcac tattttgctg gattatatgt
    18121 taacacaatt tatacgcgtc gttgataagc tcatcgtgta tccggaaaac atgatgcgca
    18181 atattgaacg atccaaaggg ttgatcttca gtcaaccggt attgctggcg cttgccaaaa
    18241 aaggactaaa acgcgaagag gcgtataaaa tcgtccaaac tacggctatg gccgtatggg
    18301 aaacaccgga cggtaacttc aaaaaagcca ttacgaccaa tgcagaagta acgtcgcgcc
    18361 tatcgaagga agaaatagat gaatgttttg atctgaagca tagcatgaaa catatcgggt
    18421 atctttttga acgcgcagga ataaaatcat agaattaatt taagtatagt gcggcatccg
    18481 caggaggtta ttttggaagt caatatcaag aaactcgaga aagaaaaaat atttcttgaa
    18541 ggaacgatta aaaaattcta ctcaacaaat aatcctgatt atttgttgat ggaatgtaaa
    18601 aatgatctcg tgccaatgga tacaaaaaaa gtaatgaaag cacgcgggaa aggtcagatt
    18661 aacacagagc ttacgtctta tgtatttcag tttcttgaga gttatcatat ccccacgcac
    18721 tacgtcggca aatacgatga caaatccctc gtggtacgca atcttgaaat gattcctatt
    18781 tatgttgtgg tacgcaatat tgcggcaggc agtttcgcca aaaactttaa aatgagcgaa
    18841 ggtgatcccc taccagctcc gatcattgaa ttttatttta aaaatgataa aatgggcaac
    18901 ccgatggtaa atgaatacca tttgtatgcg ttcggctcgg ccaatcagga tgaagtgcgc
    18961 acaatccaaa ggctttccgg taaggtcaat gcgattgttc gtaatttctt cgaacggcgc
    19021 ggttttcagt tagtagacat caaacttgaa tttggtcgac aaaaaggtaa gatctatttg
    19081 gctgacgaat tgaccttaga tacttgccgt ttgtgggata ttaaccgcgg ccgcaaattt
    19141 gacaaagaaa tagccgctgc taaagaaaat gatttgcata aaatttacgg cgaagtacga
    19201 caaacgataa cgcatcctaa aacacgggat gagcgaccaa ccattcacga ttaaccggag
    19261 attttgcttt gaaaatatcg cgcgaaggcc ttgtaacgat tttcatcgta aacatactcg
    19321 tttcgatagc tatcatcatg tatgctacgc tgggttccgg atggatcgca ctcgtgatcg
    19381 cattgctttt agtcgaactg ctcgtgctat ggttctttcg cgatcccgaa cgaacaatcg
    19441 ccggtgatga caatacgatc attgccccgg cagacggcaa agttgttttg atacgcgata
    19501 tacgcgaaga taaatacctc aagtcggagg ctacacaaat cagtattttt ctgtctgtat
    19561 tcaatgtgca cgtcaatcgc tatcctttag gtgggacgat cgctttttat gagtatatga
    19621 aaggtaagtt tatggtggct tttgatgaca aagcatccga gctgaatgag caaaccgtcg
    19681 tcggcctaga gcatccacgt gtaaaaattc tattcaagca aataaccggt cttcttgcac
    19741 gtcgaattgt gttttacggg aaacaaggtg accgtgttga tcgcggtacg cgctgtggaa
    19801 ttatgaaatt cggttcccgc atggacatca tcgttccaaa aaacgtagac atcaaagtta
    19861 aagtcggtga cacgacaaca gccggagaaa ccatactcgg catcataaag taatcaggga
    19921 tttatctttt gaaagcctcg ccaaaaaggc atttgccatc gctcgtaacc gcgctcaatc
    19981 tttttgccgg ttttctctct gtcatcagtt ccattcgggg tgattatatg gaagccgcct
    20041 ctctgattgt cgttgcggcc attatggatg ttttggacgg taaagttgct cgcctcacca
    20101 agtcgagttc tgaatttggg gttgaattag attctcttgc cgatgtatca tcgtttggat
    20161 ttgctcccgc tgtgctgatc tatagagcat attttgaacc ttggggtatt tggggaatct
    20221 taataagctt cacgccgctt gttttcgggg gaatccgctt agcacgtttt aatgttggcg
    20281 tagcagacca tgcggaaaaa gacagctatt ttaccggttt accgattccc tcgtcggcca
    20341 ttaccatcgc ctcttttgta gcatttgaaa tggacgtatt tcaagcctgt aaccatattt
    20401 tcgtgatggc tatagtcact ttgcttgtat cgtttcttat ggtcagtaaa attcgctatg
    20461 agggaatgcc taatttttca ttcaggaaaa ccgaagatcg catcaaactt gggcttttga
    20521 tagtttgttt tattgctttt ttgatttatc ctcaacgtgt tgcgtttcct gtcatgatac
    20581 ttttcatagc ctatggaatt ctgatgggta ttctggcaat attttctaat aaagagtctg
    20641 acgatgatac gcaagatatt ccggctatag actaacgact aagtcggaat aattttcgaa
    20701 aactttctct tacctacctt aatcacaatc tccgattcga tttggataac tgcacgatca
    20761 tccgctattt tttcaccatt gatactcacg gcaccttgct gaacttgtct ttgtgcttct
    20821 ttgccggatt caaccaattt acactcaagt agtagttttg aaatgatcca cgtgcccttg
    20881 ttcaatgaaa taaccggaat gtcttccggg atttctttct ttttgaaaat cagatcaaac
    20941 tcttcttccg ccgcttgcgc acgaccgtca tcgtgataaa tagatatcaa ttctttagcc
    21001 aagcgtcgtt ttaagtgcga tgggtttatg gtaccactgt ccaaatctgt ttttatttta
    21061 tgaagctcgg ctgcatcaac attcgtggtt aattcaaaat atttataaat taaagcatca
    21121 ggaatagaca ttgcccggcc aaacatttct ttaggcgagt catcaatgcc gatataatta
    21181 tttaacgatt tgctcatttt ttgaacgcca tctgtcccct ccaataacgg catagtaata
    21241 atgacctgct gttgctgttg gtattcgcgc tgcaaatccc ggcctaccag caaattaaat
    21301 ctctgatccg taccacccaa ctcaacatcg gcttccaagg ccaccgaatc cataccttgc
    21361 gctaatggat ataaaaactc atgaatactg atcggttctc cgctacgata acgattttca
    21421 aaatcatctc gctccaacat gcgtgctacc gtataccgac tggccaattt aactacatcg
    21481 gcaaaattca tggctcctaa ccagtcagaa ttatatacga tttttgtttt ctgggcgtcc
    21541 aaaatcttag acgcttgctc cagatacgat ttaccgttaa gccgtgtctc ctccaatgat
    21601 aacgatggtc tggttttatt tttacctgtt gggtcaccga tcataccggt aaaatcaccg
    21661 atcaccaata tcgcctgatg tccaagatct tgaaattggc gtaattttct caataccaca
    21721 gaatggccta tatgtaaatc cggacgtgaa ggatcacagc ctaatttgac tttaagcggt
    21781 tttgcggttc tgagagaatt ctcaagtttt tttacaagtt cgtcttctgg gattaagtca
    21841 acgatgccac gtttgataac tgcaagctgg tcagcaatcg gaagaaacat caattctcca
    21901 taaaattcgg atgaaaagga attctgatct ttacggtagt ccctatattt tctctgctag
    21961 taataccgat ggtacctccg tgcttttcaa ttaacatttt tacgatacac ataccaaggc
    22021 ctattccttt cggtttggta gtaaaaagag gttcgaaaat acgctcaagg ttttcgttgg
    22081 atataccgca tccggtatct ttgatttcga cgattgcggc atctccgtca tgatgagtac
    22141 ggacatagat ttttccgcta tattccatgg cctggatggc attaagcatg agattgataa
    22201 gtatttgctg aatctgacct tcatccactt gattatccgg tagatcgggt gccaattcgc
    22261 gaacaacctc cacttcatcc gggaaacgca tgcgcattag cgtttcatca ataatgatgt
    22321 taaaattgcc cggtttaaaa atagttttac ggtcacggga ataatctaaa aggtcattaa
    22381 taattttggt agatatcatg acctcattat caatgatttg aagaagttct tttttctctt
    22441 tttcttcggg taaaatttca tttcgcatca acgcagcgga cgctttgata cgcgacagcg
    22501 ggttgcgtaa ctcatggccg atactcgcgg tcaattgacc cactacggct aatttttcct
    22561 gacgtaacag tttttcctga gtctccttta attgcttcag gttttgttcc agtgccgcat
    22621 ttttagtgat tagctcgtca gttagttttt tattttcttg taccaatgca taccgttcaa
    22681 aagcttgacg tacgatcgct ctaaggtcat ctgtatccca tggttttacg acataacgat
    22741 ataccactgc actattgata gcttcgatag caacattgat atccgtatag gccgtaagga
    22801 tgatattcac cgtattggga gccaatactt ttgcttcttt aagaaacgcc atgccattca
    22861 tttttggcat acgttcatca gtaatgatca cctgaaccgg ttgccttttg agtaactcca
    22921 gcgcatcatg agctccagta aaagaaaaaa tctgatattg ggattcgaat gttttaacaa
    22981 gtacctccag aacccttgat tcgtcatcaa ccagcattaa agtaggtttc atgaatgcct
    23041 catcgtacgt gatgcatgga gaccgttttc tactttctcc agcaattcat tcaaattgaa
    23101 aggtttcggc aaaataaaat ctacatatgt ggtccgcgca tccggacgct ctgtatcgat
    23161 attccacccg gtcatcatca taacaggcag atgtgggttt aacgctttga tctgtgcagc
    23221 caattcagag ccgctgatat tgggaagacc taaatccgta atgatcatgt catagcgtaa
    23281 tttattaatt tgctccatcg ccgacgatgc atccgggcaa catacgactt cataattacg
    23341 cgactccaaa atatccacca taagttcacg taaagattcg tcatcatcaa caattagaag
    23401 ccttgtttcc tttttaggat cggacaaaga ttgtgagttt tttttcgatg aatccgaacc
    23461 cgtaatacgt cgcagcgata gaatacactg tatcctgttt tcatgtgctg accaatcaca
    23521 ttcccagcca tttaacctag cggtgaaagt ataaaaatca ccgattttct cgttaaactc
    23581 tctttcggaa tcgaccttta ttagaatttt taaaaactca ccatgaggcg tgtattcaac
    23641 agttattgtc gaattttctt tgccataacc gatcatcaaa gaaaatacgg aagatataaa
    23701 ttgttgcaat cgcttaaact cagaccaaac gatttcacga tcttgccttt tgaagtctat
    23761 aataaatctt tttttgacac acgtttcatc gtttcttgcg atatctacag attcgtctaa
    23821 gaccgatgaa atatcggact ctgcattatc ctgcgactca gaataagttg tatgcaattc
    23881 gtttttttca tacatggaaa cttcttttga gaaagctaaa acagcatcta tattgttttt
    23941 gtcttgttga gttattgacg gtagcagcat ttgtgctaag cttcgagcaa tcgtaatcat
    24001 tgtacgcgcc aattcatctt gtggcgtttc cggcttaacg gcggctagag tctgttcaat
    24061 ctgagcgcgc aaattatctc tttcaaaccg aacggtttcg agagtttccg ttgcgtccaa
    24121 agaggcgcgg tgcaggcccc ataacgatga caataattcc aaaaaaggta tatttatgct
    24181 tttttcaggc tccgtcaatt tatcggcaac aataaggcct actacttgtt tgtccgataa
    24241 aacgggcaat acaatcgatg tcatcggcac tgctgatgct ttaccgtata tttgggaact
    24301 atctccgatt agaggctctg cttgcttaaa ggcgatttgc atcgcggcat tcggatgagc
    24361 cggttgtgaa tttagtgccg gattatgagt gccatttttt tcccatcgca gaatagagct
    24421 ccattgaccg tcattgtttg ttccaaaaca ttccaatcgc acaacatcgc actgacggag
    24481 ccactcctgt aaccataccg atgcaaaagt ttcgggtttc gcacgcatac gcaatgcgtt
    24541 caatacttcg gagagggaat acatttgttg ttcagccatc atgttataat cggctctttc
    24601 atttccagcg atttttttca cgatcaaaat ttttaaacat catggatgcc acaggacgta
    24661 tcgtgcgttc attatactta gccgacggct tagaattata aggtgtttca atctgccccg
    24721 aaacctcaaa ataaatcaat tgacctatgg gcataccctc gtatacgcgt acggggtgct
    24781 ttactgaaat ttcgagtgtc catgtattac agaaaccaat atccccttta ccggctgtcg
    24841 cgtgaatgtc aattcccaat cttccgatac ttgatttgcc ttccaaaaac ggaacatggg
    24901 ccatggactc cgtgtattct tgggttacac caagataaag tttcgtgggc atcaatacat
    24961 acccttcttc gggtatttca aaatgttcga tcggattatc ttttttgcaa tctagagttt
    25021 cctcggaata caccgcgagg tatttgccta aatgcacatc ataactattg gaaccaaggc
    25081 aatcgcggcg aaaaggttca atacgtatgg tacctttagc gatttcggct aaaatctgtg
    25141 tgtccgttaa gatcattggg ataaataaaa ttaggattgt aataaaacta aatgaatcca
    25201 atattagaaa taaatataga caacactaca gcgtttatca ataaataatt tacctccaac
    25261 actatccatg acgtctcaac gaactaaaac ccaaacgccg gaaaaaatta caaaaaatct
    25321 tctcctgtgg ttctataaac ataaacgaga gatgccctgg cgtacaacac aggatgccta
    25381 tcgcatatgg gtttcggaag tcatgcttca acaaactcaa gtggagcaag tgcgccccta
    25441 ttatcaaaga tttatggtcg cattccctga tattttaacg cttgccaatg ctaaacttga
    25501 cgaagtttat aaactttggg aaggtttagg ctattaccgt cgagccgctt tacttcacaa
    25561 agcagctcag atcgtagtta ctcaatatca aggtcgtata ccggatcaat acgaatttgc
    25621 acgtagctta cccggtttcg gagattatac tatcggcgct gtacttagta tcgcgtacca
    25681 aaagaaatta ccggccattg acggaaacgt aatccgtgtt atgagtcggc tttaccgaat
    25741 cgaatctgaa gttcatctgt ccgcaaccaa acgacaaatt gaaagtttag tgcaatccat
    25801 gataccggat gaaaacccgg gcgattttaa tcaagcactc atggagctag gcgctttgat
    25861 atgcacgccc caatcacctc aatgcacccg ctgtcctgta caccgatttt gcaaatctta
    25921 taatgaatta cccaatccta atattttacc taacaaaaaa aagaaaacca tcaaacctca
    25981 catgcatatc gctgcaggaa tagtttttaa aaataaaaga attcttgcgg ctaaacgccc
    26041 gtccgatgtg attttgggta atctatggga atttccaggg gggaaaaaag aatccggtga
    26101 atcgctttta aaaacttgtg agcgcgaatt gcaaaacaaa atcggtatta gggccaaaat
    26161 cggtaaatgc ttcttagtac acaagcatgc gtattcacat tataccatta cattacattt
    26221 ctataaatgc gactacattg gaggtcaacc gatcaaacgc aaacactcag ccttacgttg
    26281 ggtttacccc aaaaaggcac tggaattggc atgggctacc agccaccgga aagcattaga
    26341 gaagttgcta aaggatgcga tttattaatt gggtgtattg gacgtcgtcg caccggtgtg
    26401 ggctgaatcg ccaaaaacaa ccccatttcg taatccggta ctgtccgcag aattatgaag
    26461 tgtcgaatcc ggtttatgtt ttttaacaag gtctttgttt ttgtatgtcg agccgaattt
    26521 gtccgtccgt tttgttccat ccggcaactc aaccgaacgc gtccacataa cgcggtagtg
    26581 ttcatttttt tcgccgactg tcccttgatc tatcgggcct tcatactcaa ctttccgtcc
    26641 gtcctttttc ccaaaaagtt tgaaatacat tttggttcgt ttgagatcta caagattttg
    26701 aatgataatc gtattcttgg tatcgttttc aaataccaaa tccggtgccg gtataaaaat
    26761 cgtcgcatcc agcccaagcg gttcataata tttaacagca tacgaatggt tcgtacgttt
    26821 tttgacattc aatccggcaa aaaaaaccgt tctaaaaagt gtacttgaaa cctgacatac
    26881 tcccccgccc caattaggct cgctgctgtc tccgtttact atcacaagct ctttgaggta
    26941 tccggaaaga ctgtccactt caccgagata ttttaaaaat gaaaaatctt cgcccggttt
    27001 aaccacaata ttttgaaaat tttttgctgc gtggttaata ttaaatttac gattttccgg
    27061 tgaaccaaaa taatccgtta cgccggttgc taatacgtcc gtaataccaa agggattgtc
    27121 aggatttgaa atcccgccgg taagatgagc tactactaaa tctgcgcgaa tattgccgac
    27181 cgtatccaat atccccttca tgacggattc gttgatattg atgatcgagc gcggtatgtc
    27241 aagataatat ccatctcggg caacaccttc aacaactatc ttaccattgt catcacgaat
    27301 tgtaacatct tctttagcac gatacatata cggcgcaacg cgacgattga gctcttcacg
    27361 caacatctct gcattaagca ccggatataa aacaaattca ttgccctccc acactttgcg
    27421 gaattcgata aattctctcc gatcagatat tgcaaaagcc cacgaatcgt agttatagcc
    27481 atcgtatgta taatacatac gaacgttttt cccatacgcc gcgtaggcac gtcgaatggc
    27541 tttggttgta gcggctatcg tgacatccgg ttctttacta agtatatcta caggtatatc
    27601 tttggacgcg aaggatcgga tattttcaga aaatgacttt tttgcttttt cttcgtcaaa
    27661 tccaagtccg ccccgccctg caacagcttt gaattgacga tcggcccaag ctacagtagc
    27721 gtcaatcggt tgattattct taagctttgg aattttgttg atgatagttc ggacaaaacg
    27781 attttcgtcg taccaacttt ctaacggaat tctaactgaa aaaaacatag atgccgtgcg
    27841 tgtttggacg tttttcaacg gatccgggtt gtggcctcgt tcgaaagctg ttactatggc
    27901 tttttctata tcaaaagtcg gctgaaactc ggtattttta gttaggaact cgccttcctc
    27961 cgtcaataat cgaacatctc gatttttaaa ctcatctaca taaacagtca atgcctcgcg
    28021 cgcctgtgaa acggtcatac ctccgactga aacgtcatgg accatagtgt tccatggaat
    28081 cctgttattg agatacaaat tatttaccga aacgataaat aaaatcaatc cgctagctaa
    28141 aacaaaataa tacagcgacc tcataatttt atgaggaatg tacgtacgta cctgtaaact
    28201 cggcgccgac gaaagattga cctgagtttg attttgatca attatgtatt ctacttcagt
    28261 tgtgtcggcc atcgtcagag ttacccctat cttttttcgc aaaaaacgta ataaataggt
    28321 taaatacgcg cttgtataac gtaaaaaaac tttttaaact ggtgcaatat aatttattcc
    28381 gccatacatc tgcaagaact atcgtgtatg tgtacaataa cgttatcttt ggtttaatgc
    28441 tcatatgcgg gtttctttag aaaaaaagat tttagtcacc gccgccgctt cgatactgtt
    28501 cattaccggt atcgcaattt tcgggtttca ttatttttca tcacgccttt ccgaacaact
    28561 gatagacaat aaaatcggac aagcgctggc tagcggccgt caaatagccg aaaatctgat
    28621 cgaagacgag cgtcgcatct tttcggaaaa tgcgcgtgcc ttttcacaaa gacctgaatt
    28681 cactgatttc attatccgtt tgcaaggtca aaaactgacc tccgatcagt ttcaggcgtt
    28741 tatcggtttg cctttattta ctcaactccg tgtagatggc gtttttttgt atgacgagaa
    28801 aggtcgttgt ttattccgac atttaagcat actagacaaa cgtctttcgt ccgtatttcc
    28861 cgatagtctc aaaacagatg aacggtttgc aagatctacc tctgcttacg acagtatcaa
    28921 tcgatccctc gtgtacaaaa cgacactggc gttgccttca caacagcagg gttaccttat
    28981 cacggcgcgg atggtggaaa aagagttcgc caaaaaatac gaacgtattg tcgcagcgaa
    29041 caatcaatat gctttgctgc tccgcgacgc agataacatg cgctttactc atctatggat
    29101 attcatcacc gcggtacttg cacctgcatt tatatttatt ttacttttat ggttttttat
    29161 cggacagatg ttcaaaccgt ttcaggcgct tagtcggcag gcttccaata ttgctaaggg
    29221 acaaatggac tatagtttac aaattgacac aagggatgaa tttggtgctt tggctgccgc
    29281 tcttaatcag atggttcaaa acctccgtga tgaacagcag cgcattttac aacttgaaaa
    29341 aacagcgaca tggcgtgaaa tggcgcaacg tatggcgcac gagataaaaa acccgcttac
    29401 tccgatacaa cttacgattc agcaaattaa agattcatac gccggaaatg atgaaaaata
    29461 tcggaaactt ctcacggaat gcacaactat catcgaagaa gaaattgaaa cattgcgcaa
    29521 tttgaccaaa gaattctctg attttgcgcg catgccggtc gttgttctta agccgtcgtc
    29581 tcttaataaa cttattgaag atatcatcct gatgtatgca gccatcccct tcgaaaaaaa
    29641 attagatgat cgccttccat ttattcccct tgataccgag gcattcaaac gcgtattaat
    29701 taacctgatt gataatgcgc tggccgcagt ttcaaacaaa caggatcgcc tgattcgtat
    29761 agcaacgata gatgtgaaca cacacgtaca acttattatt gccgataacg gatacgggat
    29821 tcctaaatcc aatctcgaac gtatttttga acctcatttt tcaacgaaaa caactagtat
    29881 gggacttggt ctggcgatcg taaaaaaaat aatcgaagag catcaaggca ctatctctgt
    29941 cgatagcatc gagaaccttg gaacaacttt taccatcact ttgccaaaag aaaatccatc
    30001 ttaaaggaga gtatatatgg cgtattggat cgtcaaatca gaaccgtccg tttattcatt
    30061 tgaacaattc caaaaagaca aaacaacggt ttgggacggt gtcaaaaacc cggccgctct
    30121 tattaatttg cgatccatga aaaagggcga taccgtactt ttttatcaca gcaacgatgg
    30181 taaatgcctt gtgggaacag ccgttgtaaa taaagaagcg tatcctgacc ctaaatcgaa
    30241 aaaccctaag cttgttgtgg tggaactgtc ggtgggtaaa gctttagcta agccggtaac
    30301 tcttgcagaa atcaaatcta acacaaaact cgctcagtta gagttggttc gtatcagccg
    30361 cttgtctgtc agcaaagtac cggatactct ttggaaagaa ttgatgaaaa tggccggtga
    30421 aaaatgatta taaatttata ccggtgatca gccgcagcgc ttctcgatat ttctcgcctg
    30481 ttttcttaat aatatcttcc ggcaaaatgg gtgcggctgt attattatcc cacttaatag
    30541 ctaacagata atccctaaga aattgtttgt cataactcgg ctgtttgtca cctggtttat
    30601 attgatcagc gggccaaaat cgcgatgaat ccggagataa aagctcatct atcaatatca
    30661 attcaccatc tttgatgcca aactccatct tcgtatccgc aatgataata cctttggaag
    30721 ccgccagagt ttgagcaaat tgataaatct taatgctcgt agcgcggact tgttcagcaa
    30781 catctttgct tattgtttta cacatttcat caaaggtaat attctgatcg tgaccttgtt
    30841 ccgctttcgt cgtcggcgta aaaataggtt ccggcagttt atccgattct tttaaacctt
    30901 gaggtaaaac gacgccgcag acggctccgg ttttttgata ttcgttccaa cctgagcctg
    30961 aaatatagcc acggacaatg cactctaccg gcagcgattt cgctttttcg acaagcatac
    31021 ttcgtccttg aagttcatca cggtaggcct tgcaaggctc cggatatttt tcaacatccg
    31081 tagtgatcac atggtttcga ataatatgac gtgtctgatc aaaccaaaat gaggccatcg
    31141 tattgagtac tacgcctttt tgcggtatcg tatctttcat gatcacgtca aacgctgata
    31201 tgcgatctgt cactacgatg agcagtttgt caccgagatc atatacgtca cgaacttttc
    31261 cgcgacgcat caattttaag tttttaaaat cagtctgctg taccggtgca ttagacttca
    31321 taatgatcta agcctttccc aaaaaaacat tgtttcattt tcaccggact ttaaatatcc
    31381 tgctttttcc aatacacgat gggattggaa gtttgattga tcagtttccg ctatcacccg
    31441 acgaacttca ctttgctcaa aagcccattt tgttatgcct tgtaatgcct ctgtagcaag
    31501 gccaaaacca cggtacattt ccgccgtatc gtagcctact tctacggttc cctgtatatc
    31561 tggcgctcct ttaaacccga tcccgcctac gatatttttg gattttgttt cgacagtaat
    31621 ccataccagc cacatgtact cgcttggatc ctgttcgatc aaacctataa aaaatggcaa
    31681 cgcttcaatc aaatcgagtg caggccattg ctccggaaca ttaacgccta gatgctgcgc
    31741 gaatttatta cgattttcac ttagcaactt cagtgactct atatccaaag cttgaagtgt
    31801 caaacgaggc gttgtaacta ttatttgccg tgacattgtt tatatttttt tccacttccg
    31861 caaggacacg ggtcgttgcg tccaactttt tgttcggcac gtatcggtgt tgttgtcgat
    31921 tgcacgggtc gttcagcctg ttcttccccg ctcccgctaa agcccattcc ttccgatgat
    31981 gcatgcacag ccttcatagc acgtgcgcgg cgaatcattt gttgttccat ctctgcagcc
    32041 ggttctgcac gaaaaacaaa ttccacaatt tcggatgtga tgcggtcaag caattgcatg
    32101 aacatctcaa aaccttcggt tttgtattcg atcagcggat ctttctgacc ataaccgcgt
    32161 aagtggatac cttctttaaa ctcttccatt tcacgtagat gctctttcca acgttcatca
    32221 atggtcttga gaacggcgat acgctcgata cgacgcatgt tttcagatga ccatacttct
    32281 tcgcgctgtt gataacgctc catgagcata ccgcgaatgt gaacggaaaa ctgctcggca
    32341 tttaatttat aaaactcatc ttgtgcaacg gcaataccac aggaacgcaa cagtgtctcc
    32401 gtcaatccaa ccaaatccca atcctcggga tgcggttgat ttttgacaaa gcgatcattg
    32461 agcatttcta caaattcgtc cagaacatca aggaactcta ctttgatgtt ttcacccatc
    32521 agtgcacggc ggcggcgact ataaatgatt tcacgctgtt gattcatcac gtcatcgtat
    32581 tcgatgaggc gtttacgaat gctgaaatta cggaactcta cccgcttttg cgctctttcg
    32641 accgctttgg ttatccagcg gtgtgatatc acttcacctt cttcggcacc caatttgtcc
    32701 ataacccgcg caatacgatc ggaaccaaaa aggcgcatga gatcgtcttc gagggaaata
    32761 aaaaacaccg agctgcccgg atcgccttga cgaccgcttc gcccgcgtaa ctgcaagtca
    32821 atacgcctgg attcatgcct ttcggtaccg atgatctgca aacctccggc ttctacgaca
    32881 ccggagccca atttgatatc cgtaccacgg cctgccatat tggtagctat cgttacggcg
    32941 cttttttgtc cggcgttgga tatgatctcc gcttcacgct gattttgttt ggcgttgagc
    33001 acttcgtgtt tgatgcccgt gcgagatagc atacggctta aagtctccga aacttcgaca
    33061 gaggtagtcc cgaccagcgt gggtttaccg gctttatggt tttcacgtat cagttcaatg
    33121 acagcgttgt atttttcccg tttagtgcga tagatcatat cttcaaaatc ttcgcgttga
    33181 atgggtttat tggtcggaat tgccgttaca tcaagtttgt aaatttcata aaactcagtt
    33241 gattccgttg aagccgtacc ggtcataccg gctaatttgc tatacaaacg gaaataattt
    33301 tgaagtgtga tagtcgccaa cgtttgcgta tctttttcga cttttacatt ctctttggct
    33361 tcgatggctt gatgtaaccc atcactataa cgtcgtccgt gcaaaatacg gcccgtaaat
    33421 tcatccacaa tttgcacttt tccgtcttgt acgacgtatt cgacatcttt ttcatagagt
    33481 gagtaggccg tgagcaattt ttcgatactg tgaatgcgat cactgcgctc ggcgtacagc
    33541 gtgccgagtt cttctttttt gattgctttc tgatgatcgg ttaattcttt attgccgtca
    33601 attttgatga tttcctcaga taaatcggga atcacaaaca tctcaggatt ggacggtgac
    33661 agggcgttgc gtcctttttc agtaagatct ataccgtgat ttttttcgtc gatagcgaaa
    33721 aacagctctt catcgatttc gtgcattttt ttatcgcgga gatattcgct ttcgacactt
    33781 tgtatcaatt gcgcaacacc ttcttccgtc atggctttca tcaatcgttt gttttttggc
    33841 gcaccgcgct gtgccatcag cagtttgatg ccggcttctt tattttttcc ttcagtgatc
    33901 agcttatccg cgtcggccac caactggctc accaaacgtg tttgcaagtc cacgaggcgc
    33961 cgtacacccg gattactttc ttcgtatttc tgattggtcg gagtatctac agcgccgctg
    34021 ataatcaaag gcgtacgcgc ttcatctatc aacacagaat ccacttcgtc cacgatcgca
    34081 taatgatgcc cgcgaatctg tacaacgtca tcgggtgaag tagccatatt atcacgcaaa
    34141 taatcaaaac caaattcatt attcgtaccg tacgtaatat cacaaccgta ggctacacgg
    34201 cgttgcgtgg ggttcatatc gttaagaatc acgccgatcg taagccccag gaattcataa
    34261 accttcccca tccattcttt gtcacgcaaa gcaaggtaat cgttgacggt aacaaggtgg
    34321 acgccgtgcc cggccagcgc attcagataa accggcgcta ccgccaccag tgttttacct
    34381 tcaccggttg acatttcgga gattttacct tcatgcaata cgatcccgcc gatcatttgt
    34441 acgtcgtatg gtaccatatc ccactttact tcacggccac aaaccatgta tttttgccca
    34501 accaagcgtt tgcaggcttg ttttacacag gcaaaagcct cgggtaaaat ttcatctaaa
    34561 atatattgtt ccgcttcgcg caaagttttt tcggcaactt taagttcttc aaccaaagtt
    34621 ttagaacgat tcggatcggt attggttcgt atcgtttctt tgatcgcctg tacagcggct
    34681 atttcttctt tcgtattttc tcgtatccga tcacgaaact cgttggtttt ggcgcgcaat
    34741 tcgtcgtccg atagggtgtc gaattgttga aagttttgat tgattgcatc cacgatcgga
    34801 tagagctttt ttacatcgcg ctcgtgtttg tttccaaaaa ttttggataa aaggctttga
    34861 atcatcgtat ctgtatactc cctgtaagtt tctttttatc gtccgcgcag aaggcgttcc
    34921 gccaatatca ccggtaatcc ccgttcgcgg gttttgcgtg ccgtggtctg aacatcatac
    34981 tcactgcgaa tatttttata atcccatttt tctgtatcaa acaatccaaa actcaaacgc
    35041 gcatcgccat cccgcggttg gccgacgctg ccgacattga tgatgaactg gaattcgcgg
    35101 gttatcgccg tcgcatgcgg agaattgcaa aacacacgcg gcgaatgcgt atggcctaca
    35161 aaacatatcc gctcttgaaa tgcatcgaat tccggtaagg cgtcattgat cgtaaaaaga
    35221 tacgtccact tggccggatt catcggcgat gcatgcacaa agagtaaatc ctgacgttgg
    35281 ttgcggtatg gcaatccttg aagaaaagat aaactttcgg aactcaactg ctgatgcgtc
    35341 caccataccg cttgtttggc cacgggatta aaattttcgg tagcggacat atcgattacg
    35401 gcattgtcat gattacccat gatcacggtt ttacagtgct tgcgaattaa atccacacac
    35461 ggttcgggat ccgggccgta gccgacgatg tcaccaaggc acacaatttc atcggttccg
    35521 agttcgccga taatatccaa cgcctgacgc aacgcttcta tatttccgtg aatatccgat
    35581 atgatcgcaa tgcgcatact aatttccgaa gctgattccg atttgtcgag ctgattttac
    35641 cgtttcactg tccgggttaa tataacggat tttttcgacg gcattttcta tgctgacgtg
    35701 aatgatttct ttattacggg cacttgccat gtaaccaaac attttttgtt gtgccaattc
    35761 tacggcatac gcacccatac gcgaggcaaa aaggcgatca atgtgcgacg gaggtccacc
    35821 gcgctgaata tgccccagta caacgcaacg cgattcgaca cccgattctt tttcgatctg
    35881 agcgctgacc cagttaccaa tgcccccaag acgctcggcc ccgccgacgt tttgttcggc
    35941 cgtttcaagt atcatggttt taccgccgac cggcattgca ccttcggcta ccatgatgat
    36001 gctatagttt ttaccttgtt caatacgttt ctgtaggtgc gcacacactt tggatatttc
    36061 aaaagggatt tcaggaatca ggatgacatc caccgcaccg gaaataccgg cttctaatgc
    36121 aatccaaccc gtataacgcc ccatcacttc aacgaccata gcacgatgat ggcttttggc
    36181 tgtagtatgc agtttatcaa gtgcatccac ggcgatggta acagcggtat tatatccgat
    36241 cgttacatcc gtagataaaa gatcattatc tatagttttg ggaataccta cgatcggcgc
    36301 gcccttacgc cataagtcac ggctgatagt ctgcgttccg tcaccaccga ctacaatcaa
    36361 ggcgtccagt tccagctttt tgatattggc taatacaacg tcggacaaat ctttgatctc
    36421 tgtcgtaccg ttctttttga cgggatattt gaaaggatta ccatgattgg tcgtccccaa
    36481 tatcgtgcct ccgagaccga taattccttc gacgtcttta agttttaaaa cttgtgtttt
    36541 ggaggtatct agcaaacctt caaaaccgtc ctgaatgccg ataacttccc agccgtagtt
    36601 gttgatcgca gaaacagtga ctccacgaat tactgcgttt agtcccgggc aatcgccccc
    36661 gcctgtagaa ataccgatgc gcataacaca cctcaaattt aagggacgca atttacaaaa
    36721 ttatcaccaa taaacaacca ccattcataa aaaaaccgga catccgtaaa ggtatccggt
    36781 ttgtataata tattattccg gcgtagctat tgttttttct ttaggaacat gcccgttatt
    36841 ctccggtaag ggcttttctc ccagaatctc ttcgatttgt ttcttatcta gcacttcttt
    36901 ttcaagtaaa cgctgagtta gggcttccaa ctcctcacgt cgatcgatta aaagttgttt
    36961 ggttttttcg taagccatat ccacgatgga tttgacttcg ctatcaatta gtttagccgt
    37021 ttcttcgcta taaggtttca tattgacgcc gccaaggaaa ttttcacgac cttgttcggt
    37081 aaaggataaa ttgcccaatt tatcgctcat cccgtacatt gtcaccatac gattggcaat
    37141 ctcagtgacg cgttcgagat cattagaggc accggtagaa actttaccta aaacgatatc
    37201 ttccgccgca cgcccaccaa gcagactgca catacgttcg atcaattcat ctttggataa
    37261 aagataacgg tcttccagcg gtgtctgcag cgtataaccg agcgcaccga taccgcgcgg
    37321 cacgatggaa actttatgta cggggtccgc attttcaaga taatgcccaa ccacggcgtg
    37381 accggattcg tgatatgcca cgatgacacg ttccctcggg ctgataattt tatttttctt
    37441 ttctaatcca ccgataacac gttcgatggc ttcctgaaaa tcactcattt cgatacgttc
    37501 tttatcttta cgggaagcca ataatgacgc ttcgttgcag acattggcga tctccgcacc
    37561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tccacaaagt ccgaaccgct
    37681 gagactgaaa aacggaacat tggcctcacc ggcaacggct tttgccaaga gtgtttttcc
    37741 cgtacccgga gggcctacta ataagacacc tttgggaatt ttagcgccta acttggtata
    37801 ttttttagga tttttgagga aatccacaat ttcgcgtact tcttcgataa cctcttcaac
    37861 tccggctaca tcttcaaagg taacccgtgt tttagtatca gcggcatata tacgcgcttt
    37921 atttttgcct atgttcatca cattggcacc accgcccatg cgtcggaaaa taaaacccca
    37981 aacaaaaaat aaaagtatca gagggaatac ccaatacagt agtagatttt caagccaatt
    38041 gctgtctatt acgcctttgt agttaacacc gtacttctgc aaatcttcaa ctaatttttc
    38101 atccggtaag gcgattacaa caaacggctg acgcatgtct tttttaaacg tattctcaaa
    38161 taattgaccg agacgaatac gttctgccaa atccgtaggt ttttcggctt cttttgtttg
    38221 catcgccgct atggcgctat cggataattt aaaacggccg aatatttttt caccggtgat
    38281 ttgtacttca tccacgcgac cggcctgaag actatctcga aaagatttat aagaaacttc
    38341 aatatcctgg ttggaagaaa acaatatcgg attgagcagc ataaacacca gcaatatgcc
    38401 gataatgact ggtatttggt atttggagtt cattcgacta accggttacc tttctgtatt
    38461 tatttacttc gagtaagcaa tctttttacg tagcgttttt tttggcttta tttttccgtt
    38521 accatgcgaa gaatgaaccg cacgtttgtc cggtcgtaaa gcattttcga tgactttaga
    38581 aacctcattc acaggtataa acgtcatttc tttacgaatc tgtgcaggta cttcatcaag
    38641 atctttttcg tttttatccg gcaaaatgat cgttcgtaat cccgcgcgat gagccgccaa
    38701 aattttctct tttactccac cgattggcag aaccgtaccg cgtaatgtga tttcacctgt
    38761 catggcgaca tcgttgcgaa cgagtcgatc cgtcagtaaa gaatacaacg ccgtgtacat
    38821 cgtgataccg gccgatggac catctttggg tatagcgccg gcaggaacgt gaatgtgtat
    38881 atcaattttg tcaaaatctt ccgtgatacc caatgcttcg gcttgcgact tgatttgact
    38941 taaagccgct acggcgcttt ctttcattac atcgccgagt tgaccggtca atgtaagcgc
    39001 acctttaccg cgcattttac tcgcttcgat aaataaaata tccccaccgg cagccgtcca
    39061 cgcaagcccc gtgacaatac ccggtttttt gatacgttcg gatgcatcaa ggaaaaactt
    39121 cgcgcgaccg aggtattcgt gaagattttt tccgtcaatc gtctttttcg taattttatt
    39181 ctccgctact tcacgcgcca cacctcggca aaccgttgcc atttcacgtt ccaagtttcg
    39241 caaaccggct tcccgtgtat attcgcgaac tatcatgcga acggcatctt tagtaaaacg
    39301 aatttgattt tctttcagac cgtgttcttt ggtttgtttt ggaatgataa atttttcagc
    39361 gatcttaatt ttttcttctt ccgtgtaacc cggaatttct atgacttcca tacgatcgcg
    39421 aagcgccggt atgatcgggt cagccaaatt ggccgtagca ataaaaagta ctttactcaa
    39481 atcaaaaggt acttcgaggt aatgatcgga aaacgaatta ttctgctccg gatccaaaac
    39541 ttccagtaat gcacttgacg gatcaccccg aaaatccatt cctaccttat caatttcgtc
    39601 cagcatgaaa acaggattgg atgaaccggc tttttttatt ccttgtaata cacgaccggg
    39661 caaggcgccg atataggtac gacgatgacc tcgtacctct gcctcatcgc gaatacctcc
    39721 caaagcgacg cgtacaaatt tgcgtccaag agcattggca atagagcgcc ctagtgatgt
    39781 tttgcctacg cccggagggc caacaaaaca taaaatcggt ccgcgcatat cgttcttaag
    39841 tttacgaaca gcaaggtatt caagaatacg ttttttaacc ttatccaatc caaagtgatc
    39901 cgaatctaac ctctcgtgcg cttctttgat attgaggtta tcttccgtaa aaatattcca
    39961 cgggatatca atcagccaat caatataggt gcgcgaaacc gtgtattcgg ccgccatcgg
    40021 cgacatgcgg ccgagacgtt ctaattcttt tttagctacc tccaatgcct ctttaggcat
    40081 gttggccttt tctacttttt cgcgtaattc tgcgatttcc ggattaccgg tttcgacttc
    40141 gcccagctcg cgctgtatgg ctttgagttg ttcacgcaaa taatattcgc gttgcgtttt
    40201 actgatctcg tcttgaacat cgctctgaat tttattacca agttccaaag tctgaagtac
    40261 tttggtaagc atgatatgcg tctgctccag tcggattttg acatccaaaa tttgaagtat
    40321 accctgcttt tcatccgtag gaatattgag cgctgctacg gcggcatcgg acaatcgtcc
    40381 gggttgttga atattgagga tcatcgtcgt ctgctcggcc gtgatatatg gcgcgagccc
    40441 cgacatcttt tgaaaaatgt ttttgatgtt attcatcaac gcgtcaattt ccagatcgct
    40501 tgccggtgca ggaatatcac ccagctcggt tacattggca atccagtacg gatcttcctg
    40561 tataaaatcg ttaatgcgta tgcgctgaac accctgcacg atgacgcttt tgcttccgtc
    40621 cggcatatca aaaacacgta cgatcgtcgc ggccacaccg tattcgtaca tttcggtttg
    40681 agacggattt tcgatagcgc tttctttttg agccacgaca ccgatcagac gattggcatc
    40741 ggcgaattca gacaccaaac gtaatgtttt atcacgtccg atcgacaacg gcatgatttg
    40801 ctgcgggaac agcaccgtgt tgcgcagcgg aatgatcggc agcgaatcgg gcacctggcc
    40861 gccgcgtttt ttcttttctt tggtggaaat ttgttcttcc aacgttttcg tcataacctt
    40921 cctggttgat tagaaaaaaa gcatcaggaa aagtcctgat gcttttctct aagatcggaa
    40981 attttaattt tttaattcgg aatattaaat gcgataaaca tcgaagttcc tttgcgattg
    41041 acgaggaaca acgcggtatc gcctttactg attttgttta cagcttcaga aaattcttta
    41101 gtggatttga tatccgtacg acctacgcgt ttgataacat caccttcgcg cagaccacct
    41161 cggaatgccg gaccgttttg gtcaatcgat gtgatcacga cgccgccttc tttggcatta
    41221 aatccgtatt tctgcgccaa atcgcgactg aggttagcaa tgcccatgcc caatctttca
    41281 gcatttttct ttgcggtcgt actttcttct ttgctggccg ctacgggttg ttctgacggc
    41341 gcttcgccga gtacaacctt aatcgtcata tctttcccat cacgtataac gccgagatca
    41401 accgatgttc cggggttttt gtttacgata ttttttcgaa tttcagcact gttctcaacc
    41461 gatttgccgt taactttggt gattacatcg ccttgctgca aaccggcttt ttctgcaggg
    41521 ctatcttcca tgatcgcagc cacaacggcg cctttgggtt ctttcaaact cagcgctttg
    41581 gccatttctt cggaaatgtc ctgcatttgt acaccgacat atccgcgaac gactttacca
    41641 tttttgatca attggtccat caccgattta gcgacattgg aaggtatggc gaacccgata
    41701 ccttcataac cgccgctttt cgataaaatc gcagcattaa taccgatgag ttctccactc
    41761 atattcacca atgcgccgcc gctattgccg ggattgattg cggcatcggt ttgcagaaaa
    41821 tccacgtcgg tatccgagtt aatggacgag cgcgcaattc cactgacgat accggctgtt
    41881 accgtatggc taagctgttt gctaaaagga ttgccaattg cgacggccca ctgccccact
    41941 ttgattttat ccgaatcggc aatctttaca gcgggtaaac ctttttcatc aattttgaga
    42001 atggctatat ccgtgcgcgg atctgtaccg ataagtttgg catcgtaagt tttttcattg
    42061 gataaactta ctttgatttt atccgtgtta tcaatcacgt gattgttggt aatgatatac
    42121 ccgtcttcgc tgacaataac accagatccg aggccgctca atttttgttc ctgaggttgt
    42181 cctccgcgcg gagcgccaaa gaactgttga aagggatcat tatcgccaaa aaaatcaccg
    42241 aacggattaa attgacggga atctacgcga acagtttttt ctgtttcgac cgtgacaaca
    42301 ctcggagtaa cgtattctgc aacggccgaa aatccttcac tgaaatcctt cattgtcgcc
    42361 atcgtttctt ccaatttgac gcgttgctcg gattccgccg aggctttgcc tttatgcgta
    42421 aaattgaaat tggccgtcaa aatcacgccg gcgacaatac cgataatgat gagggaaatt
    42481 ccagcaatga gttttgatga acgtgtgcta accatggtta agacctccat tcgttgtgcg
    42541 atgttgttta cgattactat ttacgcacaa cgtcatgtca aagttttgtt aatttttttt
    42601 aatcaatgta aaatctgaaa attgttacca ctttaaatcc aaactcttag caaaattttc
    42661 aaaaagctcc ttttgatgtt tgttcaaatg tcccggtatt tttgttttga tcgtaacata
    42721 catatcgcca tgattgcgcg aagttttaac accaaaaccg gaaagcttca atattgtacc
    42781 gggttgtgta ccgggctgta ttttgacatc gatcgcattg ccatatacgg tgtgtacacg
    42841 tacgctgctc ccaaacatca actgcgcaag attggcctgc acatccgcat aaatatcaat
    42901 tcctttgcga gtaaatgctt tatccggttg cacgcgaatc gtcaggttaa ttgaaccgat
    42961 attcggattt tggattcgca acacctcgcc gtcctgtatg cccgcgggga ttttaacgcg
    43021 taaattttgg ttaccatgtt gccgcaacgt aaaatccact tcaccgccgt gaatagactt
    43081 ttcaaatgta atggtcaatt caacccgctg gttcgcctct gaagcatgtg cgccgccttt
    43141 tccaaagaac atttcatcaa ataaatccga aaaaccgcct gaactgcgaa aagacgcgtt
    43201 ctgtccgcta aatccgaaat tgtgcatgaa atcattcagg tcaaaattat cacttcccga
    43261 aaagccgcta ccctggccgc caaaacgtcg catcgtatca tattctttac gttttttgac
    43321 atcgcttaaa atattgtacg cctcggacat ctccttaaat ttttcctcgg attgtttatt
    43381 acccggatta cggtcgggat gatatttttt ggcgagattt ttatacgccg ttttaatatc
    43441 ctccgacgaa gcggattccg acacaccgag tattttataa taatctttca tcgttacgca
    43501 ttaacgggta ataagtccgt actgctgttc ttcccattgt ttgagtttgg caacacgctc
    43561 ctcggtcgtc gggtgcgtgc gaaacaaatt aacaaaccct ttgcccgaaa gcggactcac
    43621 aataaacaaa tgtgccgtag cctgattggc gtccatgggc agacgcagat tggtctgctc
    43681 cagtttaatc agagcattag ccaaacccat cggtttaccg gatatttcag caccggcctg
    43741 atcggctaaa aattcgcgcg aacgcgaaat ggctaattgt atcagcatcg ctgcgatcgg
    43801 agccactatg gctatgatca tagcactcac gatattaccg ccgccaccat tatcttcgtc
    43861 atcggaacgg ttaaacccgc cgaaaatagc cgcccattgg gccatactgg ccaaggagga
    43921 aatcgctcct gcgatcgtgg ccacgatggt actgatcaaa atatcccggt ttttgatgtg
    43981 accgagttca tgcgcaataa cgccttccat ttcatcatca ctcaaacggg ccatgatacc
    44041 atcggtcacc gctacggcac cgtgctgggg gtcgcgcccc gtagcaaaag cattgggcgt
    44101 atcggtagga atgatgtaca cgcgcggcat cggcaaaccg gcacgctgag caagattttg
    44161 taccatgctg tacaacttgg gttgatcggc gtactgcact tctttagcgc catacatgct
    44221 gagtacgatc ttatcactga accaatacgt tacaaaattc atcaaccccg ccaaaatcaa
    44281 ggcaatgatc attccgcttt taccgccgat catcatgccg ataaaaccaa aaagcagaac
    44341 gagtgttgtg aggaagaaca tcgttttgaa cgtgttcagg attttcatag gttttcacct
    44401 gtcattttag ttgcaccgtc gtccgtaaac ccacgccggt aaatgatttg aactgcactt
    44461 tagcatgagt ttttacggat gccgaagtgc ttactcaaaa atcgttactc tttgcccgac
    44521 tttgatccgc tgtccgagcc gctgttcttt ttatagtcgg taatataaaa cccggaacct
    44581 ttgaacatca ggcctgaacc ggcgccaatt tcttttactg ccggttgtcc gcattgcggg
    44641 caaaccgtct cggggctatc attgatacca tgaaattctt cgaaaacatg gccgtttttg
    44701 catttataat cgtatgtcgg catacaaatt atctttcaga ttacgaaatg ttgatgatta
    44761 gttaatcaag ttacttctta ttttcatcca ccacttcata atcggcctct tcgacttttt
    44821 ggccgctctg ctgttgcgca ccaccgtctg tcggacctgc accggtcgcg ccctgtgcac
    44881 cggcgcccgc ctgatacatt ttagaagaca cttcgttcca aatcgcgctg agcgactcca
    44941 tggccgattt catagtgtcg gtgttatccg tagcgatggc gtctttcagt ttttgcattt
    45001 caccctctaa gcgtgatttg ctgtccgcat cgactttatc tccgaattct ttgatatttt
    45061 tttccgtctg atacgccaag ctttcagcct gatttttggt atcgattttt tcacgacgtt
    45121 ttttgtcttc ttcggcatgt tcctgcgctt gattgaccat acgatcaatc tcgtctttgt
    45181 taagaccgga tgacgattcg atacgaattt gctgctcttt attggtcgct ttatctttgg
    45241 ccgtcacgtg caaaatacca ttggcatcaa tatcaaatgt cacttcgatc tgcggtacac
    45301 cgcgcggcgc aggcggaatg cccatcagtt caaaccggcc cagttcgcgg ttatcggcaa
    45361 acatttggcg ttcgccttga ccgacgcgga tcgtcaccgc cgtctgactg tcggccgctg
    45421 ttgaaaaaat ttcgctcttc ttcgtcggga ttgtcgtgtt gcgcggaatc atcgcggtga
    45481 ataccccgcc gagcgtttcg ataccgagtg aaagcggtgt cacatcaagc aacaatacgt
    45541 ctttgacttc acccgcaagg acggcaccct gaatagccgc accgacagca accacttcat
    45601 ccgggttaac acccttgtgc ggttcgcgtt tgaaaaggtc ttgcacaagc tgctggattt
    45661 tgggaatgcg cgtcgaaccg ccgacgagaa taacttcatc aatctgcgca tattgcagac
    45721 cggcgtcttt catcgcctgc tcgaccggct taacggtacg ctgaatcagc gtatcgcaaa
    45781 tttgttcaaa tttggaacgt gtcaatgtaa tgtccaaatg cttcggcacg ctgtctacgg
    45841 ccgtgatgta cggcagattg atctgcgtct gctgagagct cgacaactca attttagctt
    45901 tttcagctgc atctttgagg cgttgcaaag ccatcggatc tttgcgaaga tctatgcctt
    45961 catttttctt aaactcgtcc gcaagataat tcatgatcag atcatcaaaa tcatcgccgc
    46021 caagatgggt gtcaccattg gtcgccaata cctcaaaaac accgtcgccg atttcgagaa
    46081 tcgaaatatc aaatgtaccg ccaccaagat catagacggc tattttttcg tttttctttt
    46141 tatccagacc gtaggccaac gccgcggccg taggttcatt gagaatacgc agtacgttga
    46201 gaccggcgat ttttccggca tcttttgtcg cctgacgttg cgcatcatta aaataagccg
    46261 gcactgtgat aacggcatcc gttaccgttt gaccgagata gtcttccgca gtctgtttca
    46321 ttttttgaag caccatagcc gaaatttccg gcggtgaata catattatcc gctaccttga
    46381 cgcgtgccgt attattatca ccggctgtca cttcgtaagg tacac
//
LOCUS       DAOQGF010000009        45778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_326_length_45778_cov_7.636725, whole genome shotgun sequence.
ACCESSION   DAOQGF010000009 DAOQGF010000000
VERSION     DAOQGF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 45778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_326_length_45778_cov_7.636725"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PK937_02800"
     CDS             complement(<1..1195)
                     /locus_tag="PK937_02800"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNH31531.1"
                     /translation="MLKITNKSTFINYVLLIAAIVVMANIVSRNLFVRWDATDNKQYS
                     LSESSKEVIGKLKDPMQVKVYFSDNLPGPLANTRRYVQDLLEEYQAFSDGQFVFEFVN
                     PDADDKAKEKAQQLGIGPVPVQAVENDKMEMRNVYMGMVLLYGDKKEPIPVIQSTEGL
                     EYMITATMKKIAATNLKNVGIISPENEEISTQNLNRFLDQLYTVRSVSLGSDISADIQ
                     TIIMNGVSDSVKLDDLYRLDQFLMRGGKLFIGQSNVKDFFQQGFATDIRSNIFTFLEH
                     YGVRIGKEMITDQSCSQIQMQSQQGFFMMRTAVDYPPFPMIRNFNTSHILTQKMTVAR
                     LFFVNEVSSAEAAGTTFTPLMFTSDKTGMLNGPFYQINPTQNPMMKAFPFGTKTVAAL
                     VEGTFT"
     gene            complement(1227..1943)
                     /locus_tag="PK937_02805"
     CDS             complement(1227..1943)
                     /locus_tag="PK937_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH31532.1"
                     /translation="MPNTHMIYRKEMAVFFNSPVAYIVLAVFAVMTSIFFSNTFFLNN
                     QSDLRPLFEVVPWVFMFFVPAITMSLVARERQSGTLEFLVTLPVTDSQIIIGKFLAAF
                     TLVATALGFSLVHFFTLIAVGKHVDVGAVLCGYFGLLLVGALYCAIGIFCSTVTDNQI
                     SAFILTLLIITVFFFFFGKMVTFVPAFLAGLVQYMSVDHHLEGISRGVVDSRNLLYFG
                     SGIAFFLLVSTRLLEMRKWR"
     gene            complement(1936..2883)
                     /locus_tag="PK937_02810"
     CDS             complement(1936..2883)
                     /locus_tag="PK937_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH31533.1"
                     /translation="MIRVENLTKDYGSVRALNSISFEVQDGEILGFLGPNGAGKSTTL
                     KIITSYLAPTSGNVFINDRNILDHSLEIRKQIGYLPELNPLYYEMTVYDFLKFVAAAR
                     QIEGSAFTKRLGEVIELCGLRGVVHKTIAELSKGYKQRVGLAQAIFHDPKILILDEPS
                     TGLDPNQIVEIRELIKNLGKKKTLIMSSHILQEVQATADRMVIINKGQIVANGTTAEL
                     MSGFKGKTVVTMELLNAQEEAVRKLQGAVGGITVTQVTRDSNALMVRLEYPNQSDPRK
                     EIFEYAVKQQWAILEMSRHKVSLEDIFRTLTVEGGQPHA"
     gene            complement(3111..3698)
                     /locus_tag="PK937_02815"
     CDS             complement(3111..3698)
                     /locus_tag="PK937_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNH31534.1"
                     /translation="MNGEEYLIVHHKNLYKEIEEVIVGIDADASRTKISKEKRKIGNP
                     LLSPIDLNKAFNDAFSKRKWKESRYNYYITLDRELMEKSVLMPAKQQREFLIENGVNE
                     PIYSYNQTDFVKDKIAVEVQFGKYAFVAFDLFVKHMLFYSGGKINLGIEILPTKKMQS
                     QMSSGVAYYEGEVYNVMRQGRNSPPVPLLILGIEP"
     gene            complement(3807..3968)
                     /locus_tag="PK937_02820"
     CDS             complement(3807..3968)
                     /locus_tag="PK937_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31535.1"
                     /translation="MKRIWVLIYIHHGLIQEPEIFNDENSALLRKKEILKKINKDYDE
                     VELFEKQLA"
     gene            complement(3973..4881)
                     /locus_tag="PK937_02825"
     CDS             complement(3973..4881)
                     /locus_tag="PK937_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31536.1"
                     /translation="MPNQLKIGFLKSLEARFGKLKKLPNSLSLFEIGDGLCRIYIRYS
                     KVHSRNQSFYGIRKDDLKQLEGFNSVICFLWDTQTEPLFIPYEEFEEVFSGLEPASDG
                     QIKAQIYQEKDGTELYIANAGRFNVEAFIGWSILNLIVDKNKIIAIPDFSHSQIQTLI
                     GSIGVHHGYDIWIPNIDRNKLDWQLAEKFICKNDLPDRYEKVFDVIKQVDVLWLKRGS
                     SDLSAMFEVEHSTSIYSGLLRFNDLHLVEPNLKPKFSIVSNDLRRALFLRQINRPTFK
                     VSGLSEICNFLEYKDVFGWFNRTIKS"
     gene            complement(4954..5568)
                     /locus_tag="PK937_02830"
                     /pseudo
     CDS             complement(4954..5568)
                     /locus_tag="PK937_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(5977..6780)
                     /locus_tag="PK937_02835"
     CDS             complement(5977..6780)
                     /locus_tag="PK937_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNH31537.1"
                     /translation="MIKPGEIQQKANTVGVRDQQIEKDYILSWILFGISKHEPLSKTI
                     VFKGGTVLKKVYFEDYRFSEDLDFTLLNAELPNEQIFAWFKEVFEFIKDEANIPLDII
                     DNNEHEDGGINFYISYIGPLGGQGNNKRVKVDISRSEQLVFEPVMKNVFVDYTDLHEH
                     SLLCYPLEEVLVEKMRSVMQRMQARDLYDIWYLLDKQSMEPDFYIREFETKCRCKNLQ
                     NTDFSQKLSDRLPQYKGRWASSMGEQIKDLPDFDQVEREVLRHIKKLKH"
     gene            complement(6777..7598)
                     /locus_tag="PK937_02840"
     CDS             complement(6777..7598)
                     /locus_tag="PK937_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNH31538.1"
                     /translation="MNNKNISTRSSELLSFFNGQSRNCFDYSLAYKALPDSNASAVRE
                     LLSDMTKRGLLMRLKDGVYYIIPYEQNAKTFMPDWHLISEHLVNDAKHYIGYYSALQI
                     HNLITQPSLKEQIVVSKQIRPSEIKIKEVPFQFIYHNEKHFFGSKKIWIDSFNKVMCS
                     DLEKTLIDCLFKPDYAGGVVEVARAIYTSKDKIKYDTLLDYAKKFDSQAVIKRLGFLL
                     EILDINTQIVQELKQLKTASYVVLDTELPKTGKRISRWSIQQNLEIETIKSAIYT"
     gene            complement(7726..8922)
                     /locus_tag="PK937_02845"
     CDS             complement(7726..8922)
                     /locus_tag="PK937_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNH31539.1"
                     /translation="MKDAEASRHLRRITLESQGLTKPAPFGKGKAAVLRAMEHLGYIQ
                     IDTLSIVERAHHHTLWTRIPDYKAEYLDQLVAERKVFEYWFHAASYLPVRDFRYALPQ
                     MMRFKRGPSPYYNADPKVMQYVMDTIRTEGPKKARDFESRKKRSGSWWSWKPTKLALE
                     RLFMQGDLMISERDGMQKTYDLRERVLPETMHVTEPTPMEWAEYLVLTYLRAYGFTTI
                     RQITHLKTDERLRKNVQDILQSMLAKGAVQCVDIKGRPSVFTLSEWTEKTFKKNDPSI
                     RLLSPFDNAIIHRDRVKHFFDFDFRIECYTPQKKRLYGYFCLPVLWGDTFIGRVDCKA
                     HRKDQRLELIHLHIENTELPFEIWAEAFIESVRRFAAFNGCSAIVLTKVSPSRYKSVL
                     KKILKK"
     gene            complement(9204..10454)
                     /gene="rho"
                     /locus_tag="PK937_02850"
     CDS             complement(9204..10454)
                     /gene="rho"
                     /locus_tag="PK937_02850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNH31540.1"
                     /translation="MNLAKLKDLKISELNDIAKDLGMLSFTGMKKQDLIFTILEEQTK
                     RDGFIFSEGVLEILPDGYGFLRSQNYNYLPSPDDIYISPSQIKKFGLRTGDVISGQIR
                     PPKESERFYALLRVEAVNFENPEEAKNKILFDNLTPIYPKERLELETKNANNYSMRIM
                     NLMTPIGKGQRGLIVAPPRTGKTILLKNIANSITENHPEVKMIVLLIDERPEEVTDME
                     RSVKAEVLSSTFDEPPERHVQVSDIVLEKAKRMVECGHDVVILLDSITRLARAHNTVV
                     PHSGKILSGGVDSNALHRPKRFFGAARKVEEGGSLTIIATALIETGSRMDDVIFEEFK
                     GTGNMEIILDRRLSDRRVYPAMDINRSGTRKEELLLTQFEQSRVWILRKLMSDKTPVE
                     AMEYLLDKMRGTRSNKHFLESMNG"
     gene            complement(10841..11791)
                     /gene="serB"
                     /locus_tag="PK937_02855"
     CDS             complement(10841..11791)
                     /gene="serB"
                     /locus_tag="PK937_02855"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNH31541.1"
                     /translation="MFSRDNKTVIVFVGLQPLPESAWVHVLSSLAEITAVHDRRWLSE
                     FRAIEIYSVCTDKARMHAHIAKTLPDFLQRYAIDYFILDLATRSKKILVTDVDSTLIQ
                     NECIDELARMAGVEKTVSEMTASAMHGAIPFDEALRKRVALLRGLPASALEEVYANAI
                     RLTPGARTLSTTLREQGLHLVMVSGGFTYFTARLSEELGFHHHHANTLEIRDGVLTGE
                     VSGPIIDKETKRHVLIQHCATLRFDTSLAIALGDGANDLPMLITAGDGIAYHAKPIVE
                     QTVMNRIRYNDLTAVLFALGFADYQFSAIKEENSVKLKKT"
     gene            12054..13028
                     /locus_tag="PK937_02860"
     CDS             12054..13028
                     /locus_tag="PK937_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31542.1"
                     /translation="MEQDNKPTPKEDLAGEYADLVETPLFSRFKEWWRAKKVSLDLLG
                     VEKIIGVDNRQYKAAVKQYTNLQEEIELQYKEVVKNPDVTPEEVETAKAEREAQILDA
                     KKDFVYHMPEDYRTWFKKRKVFNSWRHAPEAWGFEESRYWGSRSGLTVREKQILIGGL
                     IIGSVVIGFIVYRIATYTPPTQAVVRELKVEKDSTMNNVDSLMRVKEQQRQQRTITER
                     NNIIINEINNGMYTTQSWNPQDEIRLIMSLYYQYTLYQTIAPADITNEGLKSVSGLIY
                     QNNPDVFTKFDAGGIPTVWKSPKKIKLNWDEIRTGVERDKKVRAIRRK"
     gene            13071..15035
                     /locus_tag="PK937_02865"
     CDS             13071..15035
                     /locus_tag="PK937_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNH31543.1"
                     /translation="MKPFSLGKKRIFYYTEDARSVRDQLEGRSFTYTGQPLVFGVSTD
                     TIINGAACTLGYTEPILGPHFLVGKGDVIHEGDVQKGNFSVVVGGESYGSGSSREVAV
                     VAHRGAGIELVIAKSLERIFRENMTFDRLYFTNDMTVMDKLLAGETVDLDVLMEKQLS
                     PLYKSVFFGGGLLEFGKKVIAGKAELPYKAVRRSVKPMTIAEKILARKAVATDAPGGQ
                     GLNYSEPGEQLFVKADFLGIHEYTGGHVRSLYEKAFGHNPTDKGVTIRGFNDHFVLIG
                     HDDVPGDVKSKRLASAQALAQELIGFCKEHNLPMHGPGGNLPAGVCHRLVVESYAKPG
                     DVIALTDSHTPTAGVMNCFAYGLGSTAIALGLGTGMIPVTVPKTVRIWIEGEDKLNLV
                     TPKDIILHIIGDPYFREMQWKKEPGDTVIMQFGGPALERFTVDELSVLTNMTVEGSAT
                     TGVVEPCTPVIRFLIEKRGMTESDVRSMLVYPDDKATYESVYKVNLADVPVTVATPGD
                     SRNRKPITAVAKTRIDNVVIVSCTGASLEDLKQVAMAIRGHKKLDRIHMVVTPSSQKV
                     YDDAKQLGMIKMFQDFGAVVTKPGCGSCIGNGPGISTKDTITASTSNRNFSGRMGGNG
                     DVFLVSPFVAGVAAVTGELTDPRGWQVVNA"
     gene            15125..16522
                     /locus_tag="PK937_02870"
     CDS             15125..16522
                     /locus_tag="PK937_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNH31544.1"
                     /translation="MFKGHPRGLKVLFFSEMWERFGIYTMLAIFTLYMGNYFGWPDTK
                     TGQIYGIFLALTYFSPVLGGYLADKFWGFPKTIMVGAIATGIGYAILAIPKPSEPLFY
                     LGLFVVAMSNGLFKANISVLVGNLYEKNSPLKDAGYNIFYMGINVGAFLAPIAATFLR
                     NSIGTEYGYNAGFGAAAAGMLFSFITFRLGQASYNASAAAVAANGSTTMATEERVSPK
                     KEKDRVIALIIVYLIVVFFWMSFHQNGFALTLFAERSTRPMDLPEGLRSVLYVILGIS
                     DPANASEAEFYQVFNPMFILMMTPLVVMFWGWLNKRGLEPATPAKIGIGMMLCGLGFA
                     IMIFASMGGGDADNKSMSPLWLISSYYVQTLAELCLSPMGLSYASKMAPYRWRGLLMG
                     GWFAATAIGNYFAGFLGSFYSTWSHTQFYTVLTVCCFFSAVMIVFFLKKLKHAAEPDE
                     PIENITGEVWGTKPS"
     regulatory      16597..16716
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            16815..18071
                     /gene="metK"
                     /locus_tag="PK937_02875"
     CDS             16815..18071
                     /gene="metK"
                     /locus_tag="PK937_02875"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNH31545.1"
                     /translation="MSHYLFTSESVSEGHPDKIADQISDALVDHFIAFDPYSKVACET
                     LVTTGQVVLAGEVKSSTYLDVQEITRGVIRKIGYTKSEYMFEAHSCGILSAIHEQSQD
                     INRGVDKKKKEEQGAGDQGMMFGYAVNETADYMPLTLDLAHKLLQELAVLRRENKDIK
                     YLRPDAKSQVTLEYDENRKPVRIDTIVISTQHDDFGSDSAMQDKIRKDMINILIPRVM
                     KRYPKYKHLFNNQIKYFINPTGKFVIGGPHGDTGLTGRKIIVDTYGGRGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGVVDEVLVQVSYAIGVAKPMGIFVNTFGTGKVGM
                     SDADIAKKVESIFDMRPYFIEKRLKLRTPMYSESAAYGHMGRKNEWVTKTFTSGDGIK
                     KTVKVELFTWEKLDYVGKVKAAFRIK"
     gene            18173..18745
                     /locus_tag="PK937_02880"
     CDS             18173..18745
                     /locus_tag="PK937_02880"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNH31546.1"
                     /translation="MIIIIDNYDSFTYNLYQYVRELAAPMSDVRVYRHDEITIPQLEA
                     LRPDRIIISPGPGSPHDAGISNDVIRYAADTVPLLGVCLGHQCLAQVYGARILRANEP
                     KHGKVSVIKHDGRGVFAGIPNPLRVTRYHSLIVDPMSLNQKIEISAQTDGGEIMGLRI
                     QGTRAESVQFHPESVMTEHGKKMINNFLKM"
     gene            18806..18878
                     /locus_tag="PK937_02885"
     tRNA            18806..18878
                     /locus_tag="PK937_02885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18839..18841,aa:Lys,seq:ttt)
     gene            complement(18940..20148)
                     /locus_tag="PK937_02890"
     CDS             complement(18940..20148)
                     /locus_tag="PK937_02890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH31547.1"
                     /translation="MRRKSSPSASISTQKSMKNRPTNTEPSPTQMDASETLLIEELKS
                     KVSELEASYIRVQDENQLLHKRLHQTSLLYKIAAMLNSTYDTTKLYNIIQNMLSDISF
                     VRHYALIERTDSGSVNVLFHTHTKDRSLAHVLDAEKEVAEKVFSTEKESYVISVNERK
                     DLLKYFPMKTEGSIVALPLTFHKKSSKVVCYFAEHSLSDGEKEFLNLITNEIGVAVER
                     ALLYQETFEISIKDGLTGIYNRRYFNDRSQREFNRAKRYKHILSFILMDIDHFKKFND
                     TYGHKVGDEVLVWVAKNLGKGLRDHDVLARYGGEEFIVILPETDVKGAAIVAEKLRKI
                     IDEQSVQINHLFVSQNPHSTFHTDRVTISLGVASFPKDGATLEEVLERTDQQLYVAKS
                     QGRNQVGYEE"
     gene            complement(20155..21402)
                     /gene="clpX"
                     /locus_tag="PK937_02895"
     CDS             complement(20155..21402)
                     /gene="clpX"
                     /locus_tag="PK937_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNH31548.1"
                     /translation="MARTVFCSFCGKSAEEVDSIISGPNGVHICNECVRNAFDMIRSH
                     TTRDVSLPLTDITPEKLKLKLDEYIVGQDFAKKSLSVAVYNHYKRINALQDSENDVEL
                     EKNNILLIGPTGTGKTLLAQTLAKFLNVPFTIADATTLTEAGYVGEDVENVLVRLLQA
                     ANYDVQAAERGIIYIDEIDKIARKGGNPSITRDVSGEGVQQALLKILEGTVANVPPKG
                     GRKHPEQNLISINTKNILFICGGAFEGIEKIIQTRVGKKNIGFTASADKKAEQSVSEV
                     LKLVEPDDLLGFGIIPELIGRLPVISPLEELSESALYSILVQPKNALVKQYKKLFALE
                     HVELEFDEKALKEVVKLAVRRKTGARGLRAIMEKFMTDIMYQIPSISHLKSVTIHKDC
                     VVSGGKPRMTFAEPEDSTVKKTA"
     gene            complement(21521..22111)
                     /locus_tag="PK937_02900"
     CDS             complement(21521..22111)
                     /locus_tag="PK937_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNH31549.1"
                     /translation="MSGSVVPYVIEQKGQMERVYDIYSRLLMERIIFLGTPINYEISN
                     VVIAQLLFLAATDPEKDIIMYINSPGGSVTDGLAIYDTMEFIKPEIQTTCTGMAASMG
                     AILLMAGTKGKRYALPHSRIMLHQPLGETQGQSSDIEIYMQEIVKMKDQLNKIIMQRT
                     GQNAERLEKDIDRNFFMSSDEAKAYGIIDEVLVRKK"
     gene            complement(22301..23584)
                     /gene="tig"
                     /locus_tag="PK937_02905"
     CDS             complement(22301..23584)
                     /gene="tig"
                     /locus_tag="PK937_02905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNH31550.1"
                     /translation="MQASVIHEDNATRKLAVEVPYDAMLPYFEEALAMYREEAQIEGF
                     RKGKAPKDVIQKKFGNMIEAEALEVVVEDFYREALEATKTEAIAIGKIENMDYKKGQP
                     LKFDVTVEIMPKYDVQNYKGLNLKREVHKITDEEVTGAFDRLRESYSTTKETDVIAAG
                     NFATVDIQVLDESGTPLIGKKYEDRRIPLTRQFVGQDFIDGLIGAKTGDMRILDVAKR
                     PPQEGEDIATIEKEKYQVTVRKIEEMQLPALDDEFAKDLGFETFELLKADMHKNLSER
                     WEQESEQKFREALIDEIIKQNEIPAPEPLVDNSLQRIGRMIKERLKGQPVKDELIAER
                     YRPSAIREVKWTLAKRKIIEQENLKVEDADIQAYREKTAKQNKVPLEQINVHFHTETE
                     QRNFEDYLLEEKAAKLIADSAKVEEVPDMLSEEKK"
     gene            complement(23632..23715)
                     /locus_tag="PK937_02910"
     tRNA            complement(23632..23715)
                     /locus_tag="PK937_02910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23679..23681),aa:Leu,seq:tag)
     gene            23837..24571
                     /gene="rsfS"
                     /locus_tag="PK937_02915"
     CDS             23837..24571
                     /gene="rsfS"
                     /locus_tag="PK937_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNH31551.1"
                     /translation="MGSSKKTKKATPKAGKSSKPAKKKAAPKTGKTLKKSGTAVNKTK
                     KSVKTVKKAAKTAVSKKTKTVKKVVTKKTKPVKKDALIAQDEMMDEDVDRLTTEEIDA
                     ELAAYESEKGKPRDPKTGKTLPESEAMQLCRRVTEYMFEKKAEDVVIADLEGLSSAAD
                     FFVICTATSDMHAKAIADNVEDRLAQEGAKLHHKEGHQSLKWVLLDYVDVIVHVFQKD
                     ARSFYDLERLWGDAKFTKVRDTAGRA"
     gene            24721..25146
                     /locus_tag="PK937_02920"
     CDS             24721..25146
                     /locus_tag="PK937_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH31552.1"
                     /translation="MTKEMWLNLPVKDIKKARAFYSHLGFAFNSERDTEHTACMLVGD
                     QDAGKETIVMLFSEHVFQSFTHHPIADTRHGSQVLFSIDAESREEVNRLAARAEAAGG
                     KVFAKPAENTAWMYGCGFCDPDGHRWNVLFMDMDKMEKF"
     gene            25067..25780
                     /locus_tag="PK937_02925"
     CDS             25067..25780
                     /locus_tag="PK937_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNH31553.1"
                     /translation="MDAGSAIRTDTGGMFYSWTWIKWKNFDLMNYNDYILAIIPVFVA
                     IDVIGFMPLYITLTDGIPEPVKSRVLRQSILTAFLVTMTFLALGKAIFAFLGISISDF
                     KIAGGIILFAIAINDLISSSDDRISNEVRDETTEIHINQNPTIGIVPIGIPLIVGPAL
                     LTTMMITIDSYGMGPTIFSVMINLLIVYGALHFSPYVLRITGRSGAKGIAKVFTILLA
                     AIGVMMVRVGITEIINGKV"
     gene            25780..26571
                     /locus_tag="PK937_02930"
     CDS             25780..26571
                     /locus_tag="PK937_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH31554.1"
                     /translation="MRNQDEEPLNEWDLPEEPESDEEDPEAAHIALTKEMYYTAIIEN
                     IYNEDTEKRREAIDLICHFECDPRLEEPLMKLVRSTDARFVLSGIVGLGNWRHTDGAE
                     VLIEMINHPTPGVLWDMEMLQEALIALGRIGGQEPLAFLKTYAHQRYHDHDGGEDTLG
                     MAAIEAITEIATHGQHGAVEFLIEGCRSLAWNMREACADSFSVVYAGKEQLPKNVYET
                     LVTLTKDENKNVRIAAYMSLDDIVGLDEKNKQILADARHKQVFGK"
     gene            26587..28077
                     /locus_tag="PK937_02935"
     CDS             26587..28077
                     /locus_tag="PK937_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNH31555.1"
                     /translation="MNLFLLVITVYVLVLIGIGVYKSFQIKSQDDFMVAGRNVSMWFL
                     VGTLVCTWVGSGTLFGSAGLSFRDGFSALWMSAGAWVGIVCVYFLAGRVRKIAEYTVP
                     DILEKRYNATARILGTITIVIAYMSIAGYQFKGGGRLLNIITRTDPHNAATGLDPFVG
                     ALITCGVVVLFTLLAGMVSIIAADLVNGLIITISVIIGVPLLLFGSDIGSWNFVTETL
                     PNDHFTALGTHNAWWVIGIFFPTFFLLMGESSIYQKFFAAKDEATAKKAVIGMVIGVI
                     FVEGMLCLLSIIGSAKHWSNPIFKTADGGIDPSMSETVVLHIARFDLPEVAGALLMAA
                     AVAIILSTANTFLMIPSTNITRDIYQRFINPQADQKTIITMQRVLIVVIALAAFLLAT
                     QFQTILAMAFTAYTMVGAGITPVLLASFLWKRVTPQGGVASMVASMIVTVGITVANAV
                     MDQPLLEADYIIIPAIISSLGCLIFVSLMTKPSPEEKYAPFMSAGQ"
     gene            28084..30159
                     /locus_tag="PK937_02940"
     CDS             28084..30159
                     /locus_tag="PK937_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH31556.1"
                     /translation="MFYRTVKKALLFVAVPLFCINNDSLLYAQTTSDSLHLSSTDTST
                     IRPDSAMVKPFPYRSVQHFFEKIDTTENIQRGDSSFRYTILPHDSLLHYYHEDFADML
                     MQITGTYLHDQGSYGKPISVTWSGLSNRHILILLDGMPLTEPDWGWTNLNYVSLETID
                     RIEVFRGNASVRYGAQAATGYINIVLRQADADKPLTSLKFRSVFDDFRDVGVFFGRNF
                     GSRVQTYVGGSSRGTPGEQLAIGLSGGTLANFNSTRYSGKNIFFGGKVLIHENIALQI
                     HRQIYTDRYDAYGRDRDPLKDEFDFSTRGAERKDERDDWQARLVVNNKAHMWTTEYQN
                     TDIVRRSTGFTYRLVPRYYGVKNQTYRTRYAGDMGVWSPVVGAAYDRTSFDTLNTRFD
                     TFQMTSLFTENTFRWASWTAMMGMRYDHHSVYDDGVTWTASVAYPIQKKLTLRADVGS
                     TVVFPSLHDEMIHTTSLYSDAFADGYTRSYFFPKDSLSLLPVTRIRTISGTMAHHSSG
                     RGLIVSATIYTNFIKDGTYYTPVHVHTDTERVVPKTKTSGRTWGFDIEASHPFSFTTW
                     TLRQSVSQGDQVFRSGTPWYRASLTGYGEKMVFQNNMRLTGTITASYWNKPTGYTFQD
                     APAMYFVPIASNRSGGFLFFARVSAYVGDLQIFYEAENIFRNRFELMDGYAVTPQQWR
                     FGLVWKLYN"
     gene            30276..30650
                     /locus_tag="PK937_02945"
     CDS             30276..30650
                     /locus_tag="PK937_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNH31557.1"
                     /translation="MADRYKQFTEYRQKMNERILAEDNKVIKRFFGLDTLTYQDGALN
                     IKTKEMLGLVASLVLRCDDCIAYHINKSKEAGVTREEMFEVFSVGLVVGGSIVIPHLR
                     RAVEFLDEVERIETTEPHSHGH"
     gene            30640..31710
                     /locus_tag="PK937_02950"
     CDS             30640..31710
                     /locus_tag="PK937_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH31558.1"
                     /translation="MAIKKYLFWGILAGIVIWLTVSRLLKEATSSPGGGNRGGGGNAP
                     MIIKAKIIKPQTLANKIQSTGSVIANEEVELRSEVSGRVVRVYFREGSIINKHDLLIK
                     INDAELTAQLERAESKRKLAESNEVRQRGLFEKNLASQNDYDIALNELNVAKADIELI
                     KAQISKTEIRAPFNGVIGLKYVSEGSYITPTTRIASLQNMDAIKLDFSIPEKYSGAVR
                     VGDDIEYRIEGSDKLYKGKVFAVEPKIELATRTLQLRAISTQIDQRVLPGAFANITLI
                     LRDIPGALMIPTEALMPELKSQRVYVYKSGTAVSQKVETGIRTDVDVQITSGIAPNDT
                     IIITGLMQLRNGLPVRISDLIQ"
     gene            31792..34911
                     /locus_tag="PK937_02955"
     CDS             31792..34911
                     /locus_tag="PK937_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH31559.1"
                     /translation="MSLSAICIRRPVLAIVMNIVIVLFGAIAFSYLGVREFPSIDPPI
                     INVRTSYTGANAEVIETQITEPLEKAINGIAGIRTITSSSNQGTSNITVEFTLDVNLE
                     TAANDVRDKVSQAVRQLPQDIDAPPVVTKADANSDAIISMTVESTTKNQLQLSDFAEN
                     VIAERLQTIPGVSSVQIWGQKRYAMRIWMDPAKLSAFGLTPLDIRNALDRENIEAPSG
                     KISGDATELVVRTIGKLITEDDFNNLIISSTGERTVRLKDLGYARLGPENEETILRQS
                     GVPMVGCAIIPQPGSNYIEIADEFYKRFEQLKKDLPKDFSLNIALDNTRFVKRSVAEV
                     QETLLIAIALVVLIVFLFFRDWIVAVRPLIDIPVSLIGAFFIMYTMDFSINILTLLAV
                     VLATGLVVDDGIVVTENIYKKIEEGLSPMEAAFKGSAEIFFAVISTSITLAAVFLPII
                     FLEGFVGRLFREFGIVIAGAVLISAFVSLTLTPMLSSRLFRRQQKHTRFYELTEPFFA
                     GMIQAYGNSLHAFMKRRWLALAVLVVSVLLIFVVGKQLQSELSPLEDRSFIRFQVTAP
                     EGSSFDYTDNFMFNMVQLVNDSVPEKRIFLTVTAPGFAGTGAVNSAFGRLVLIEPDER
                     KRSQQEIAAKLTNYTRKYSEARTFIVQEQTISASGGGARGGLPVQYVLQAPNFERLKE
                     VLPKFMEEVAKEPTFQGFDANLKFNRPELQITIDRDRARSLGVSVLDVGQTLRLALSG
                     QRFGYFVMSGKQYQIIGQVNRRDRDEPLDLKSLYVRNRTGELIQMDNLVKVSEQSGPP
                     QLYHYNRYSSATVSAGLAPGKTIGDGIVAMDRIAKKVLDDSFSTALAGASRDFSESSS
                     NILFAFILALVLIYLVLAAQFESFVDPFIIMLTVPMALAGAILSLWWFQQTLNIFSQI
                     GIIMLIGLVTKNGILIVEFANQLREKGKNKMDSILEASVLRFRPILMTSLATVLGAVP
                     IALALGAGSISRIAMGIVIIGGVIFSLILTLYVIPAIYSYMKHEHKPGQETNIAPLQQ
                     TESLSKEHV"
     gene            complement(34987..35169)
                     /locus_tag="PK937_02960"
     CDS             complement(34987..35169)
                     /locus_tag="PK937_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNH31560.1"
                     /translation="MLEKTIRVIAGTFVLISLALGYWVHPNWLFFTAFVGINLIQSSF
                     TGWCLMETILKKTVFR"
     gene            complement(35179..35484)
                     /locus_tag="PK937_02965"
     CDS             complement(35179..35484)
                     /locus_tag="PK937_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31561.1"
                     /translation="MKLIAVMTIEQYAQDLRKLFREHHVIIFSESEIRGYRSDATERD
                     SSNWFAHTASPIYSHLVFAFVDESKAAELMQAIRNHGQTMDLDHPMRAFQMHVEQFA"
     gene            complement(35486..38734)
                     /locus_tag="PK937_02970"
     CDS             complement(35486..38734)
                     /locus_tag="PK937_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH31562.1"
                     /translation="MKTGFSGRIAQAFINSKLTPLLIAGFLAIGLFATYLTPSEEEPQ
                     IVVPMADVFVQYPGAEPKEIETKVIQPLEKIISNIRGVEYVYSTSMQDQGMLIVRFVV
                     GEDAERAIIRLYDEVFKNMDKMPAGVRPPFIKSKSVDDVPIFAMTLWSARYDALDLRR
                     FAAELSSEYKKINDVSEVKIIGGRSRQVRVILDKEKMAAYRLDPLTIIPFFQSAHQQG
                     PAGKFNQGNTEYLVQTGKFLETADDVRQLVVGMHLGKPVYLKNIAQIEDGPDEIKDYV
                     TFGYGAAHAGEHKALEYPAVTLSVSKRKGSDAMGLGERLMEKSEALKGKLIPSDVTMT
                     VTRNYGETASEKVHELLKHLSAAILAVTVLVALTMGWRSGLVIFMTVPVTFAMTLFVY
                     YMFGYTLNRITLFALVFVTGLVVDDAIIVVENMHRHFKMRKLPFLQAAIASIDEVGNP
                     TILATFTVIASVLPMAFVSGLMGPYMSPMPIGASLAMLFSLFVALIITPYLAYRLLRG
                     DTHGHSEKEYRLETTGIYKLYTRIFVPLLENAKKRWWFIGSVVVLLLASMSLMYFKLV
                     TVKMLPFDNKNELQVVIDMPEGATLEKTAALSKEIAAYLRNVPEVTHYQMYTGTSAPI
                     NFNGLVRHYDLRQGSNVADIQVNFLPKGERSEQSHDIAKRIRPAIVKIGASYGARIKI
                     AEVPPGPPVLSTLVAEVYGPDYTKQIEVAHQVRTAFETSPGVVDVDWLVEDDQTEYRF
                     EIDKEKAAQSGVSPQQIYHVLGIALNGKQIGALYQAKELETVGIQLQLAESQRSSVDD
                     LKNIHVIAMTGTPVAVSDLVRIYPQTREKSIYRKNQQRVVYVTGDVAGEVESPVYAIL
                     NIDEAMKKIRLPEGYTLQTLYAKQPFLEENFSMKWDGEWQITYEVFRDLGAAFAAVIV
                     IIYFLIIGWFQSFKVPFVMMVAIPLSLVGILVGHWMMDAFFTATSMIGMIALAGVMVR
                     NSVLLIDFIELRLQDGVPLKTAIIESGAVRTTPILLTSGAVVIGAFVILFDPIFQGLA
                     ISLIGGAIASTALTLIMVPLFYYMTEKAQYESEAVPQASVPLETTENESKEN"
     gene            complement(38731..39813)
                     /locus_tag="PK937_02975"
     CDS             complement(38731..39813)
                     /locus_tag="PK937_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH31563.1"
                     /translation="MQKYFIGLSLSVMMIVMVACGSPEKNQPNQTATPVRTITIRQKP
                     VNEKTALAGTLQGDKRITLSTKIMGQVLAIHAHIGDRVAKGQLLIKIKSDDLSAKRAQ
                     IAANKIEAEAALKNMESNYHRIKELYARKSATQKEMDDTEMAYAMTQAKLKAVEEMEK
                     EINDVFGYSDITAPIDGYVVQKLTEVGNTAAPGMPLMVIEDLSSFKVIIPIPESDAAS
                     IRIQDAVEAEFSSDPKHRLNGYVSALSPSAVPGSRQYDAEVKLHGITENLKKTLRSGM
                     FVRIYAGAGQKNMITVPDSVIHRRGQLEGVFVVSAANQALLRWIRPGKSYGNEVEVLS
                     GLDFGDRVIYESTERLSDGDRVEVRP"
     gene            complement(39831..41156)
                     /locus_tag="PK937_02980"
     CDS             complement(39831..41156)
                     /locus_tag="PK937_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH31564.1"
                     /translation="MKSLCFGVFLLFVLVGQVKAGQDGTRYTLDSVLQTASRQNYDLA
                     SVQAEMDAMRADYRKSLSLFLPQLSVSEIYTRTNDPLNVFGLKLKQEIVTQADFNPVT
                     LNQPKAVDNYTTKLEWRQPLLNMDGFFGFRAASSGVKAMEEKSKRTSYYITYQAKIAY
                     YQLLVALQSREVIRKALTAAQTGSEQARNFYDQGLIRRSDVLFAELRLKELEAKQLET
                     ENMIDNANNQLKLLMGLSGDEVIIPTDTLGGPLALEMTTPHDMTNRSDIRAMRYQMSA
                     QRAMQSMAWSKFLPSINGFATYEYNDASLFGRQAKNWMVGAMLRWDIFSGFDQISAIQ
                     KSNAQYRQTETGYKKALTQSQYDLNAALRQWKVSTRQVVIAEQAVQQSEENYRVLSDR
                     YHQGLDRTTDLLMAEAALSQARLQLIHARFAWHAAMFTVELLTEQSIKN"
     gene            41315..42871
                     /locus_tag="PK937_02985"
     CDS             41315..42871
                     /locus_tag="PK937_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNH31565.1"
                     /translation="MYYLLIALVLLTKAVLAGDAPDWVIQPGKSAAFPASAYIVGYGS
                     ASAQNPDAQKIAEDNARAALSGSIVTDLRSTVISRLEEQGKNSAEYFSSVTQSSTGLQ
                     LLGISIETYKDKKLVHVLAYAKRTELSATYNKKREALISNIAAIITQAKAAEESKKIE
                     DAAALYLSLFPMYEELREAEIVLTVVHRESQVSQPDKTIPSRTEIAQTIDRLLAQSIT
                     SVEDAARAVAYQVSKQISGTTGSFLVSPMTYQDSKMSSPFARYFQQALETQFQKYAVW
                     EVARQARGFQPKSSQITRDLVIASGAGCIFEGNYWEQGDRIKLIGRLRDVQNGRIMAA
                     VEVTLDIAHIQKTGLSIKPENMLKALADQKAFAEEEVISSDIQLDVWTNKGTENLLFA
                     EGEIMKIYVRTNRAAHIRILYNFADGSRTLLYDDHYIDEAKANQVVEIPGEFECTGPF
                     GAEMMVVVASVGKLPEVKTYSVDGIAYLDEKDAKKAAYGTRGFKKKDPASSTSPQQTE
                     KKLTITTIAK"
     gene            complement(42906..43955)
                     /locus_tag="PK937_02990"
     CDS             complement(42906..43955)
                     /locus_tag="PK937_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH31566.1"
                     /translation="MKFDDLMKIFNQLLLLTDGDVSKALSYMTQIDRQYHIFNEDMDF
                     QKFYDRLVQEGYIEESQDGSAPVLSPKGERKIRQDSLDEIFSSLKKSPGGMHETNHTG
                     EGVERLSETKPYQYGDPPSNIDFMATMNNAMKRKRGEDIELREDDFEVYETEHKSTCA
                     TVLAIDISHSMILYGEDRITPAKKVALALTELILTRYPKDSLDVITFGDEAAQIDLED
                     IPYIHVGPYHTNTKEALQLARQILRKRRNQNKQVFMITDGKPSCIREEGKLYKNSFGL
                     DRKIVNQTLNEAAQCKKDNIVISTFMIADDPYLTRFVDTLTKTNKGRAFYAGLNELGQ
                     YIFVDYIKNRKKNVR"
     gene            complement(43993..45003)
                     /locus_tag="PK937_02995"
     CDS             complement(43993..45003)
                     /locus_tag="PK937_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNH31567.1"
                     /translation="MQNNDINSFFPPFKAWFMVYRILAVLVWAVLTIVLSVSLAVYQT
                     GEIDWVNFLLTAAIASVVQGFPAHIVNEITDWKSGADQYRKLGEKSGGSKVLKSGLAT
                     ITQLWHIFDVTTIVALGLMFGLSQRTSPEIFWFFGIGYFVCLFYTLPPLQFAYRPFAG
                     EWFGGFTGIFLNMTGAYFVQTGQLDSAIILMAVITGLIYIAIMMVFHYLDYESDRHAT
                     PVKCTTIVFLGLKQSKFYVLGLLTLSFLLSSAAGWIIHPLFYLFSLLSLVHFGIHYVC
                     NPFEEASILKTGKRLTYLMIAFTVSISVVVHLYFIGLILFTVLCFVLHKKFGKLGPSH
                     AV"
     gene            complement(45006..45476)
                     /locus_tag="PK937_03000"
     CDS             complement(45006..45476)
                     /locus_tag="PK937_03000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNH31568.1"
                     /translation="MKNHKDKYTHQAPVTDALFTLLTTRLANHPANSLLGFSLEELRE
                     LYACLRLTHREAITNGPRSHGTIHGGIVAALADTAAAFALSTAFEGKMSFATVDLHIT
                     YLSRAKSTIFAHAVVIRRGARINVCEVKITDESDALVATATVNFILTQPPETKS"
BASE COUNT        11970 a        10072 c        10804 g        12932 t
ORIGIN      
        1 tggtaaacgt tccttcaacg agtgccgcta cggttttcgt accgaaagga aatgctttca
       61 tcatcggatt ttgtgtcgga ttgatctgat aaaagggccc gttcagcata ccggttttgt
      121 ccgacgtaaa cataagcggc gtaaacgtcg ttccggctgc ttcggcggag gaaacctcat
      181 tgacaaaaaa caaacgtgcg acggtcatct tttgcgtcag gatatgcgag gtgttgaagt
      241 ttcggatcat cggaaacggc ggatagtcca cggccgtgcg catcataaaa aagccctgtt
      301 gcgactgcat ctggatctgg ctgcaacttt gatcggtgat catttctttg ccgatacgga
      361 cgccgtagtg ctcaaggaat gtaaatatgt tagaccgaat gtccgtagcg aaaccttgct
      421 ggaaaaaatc tttgacattg ctttggccga taaaaagttt gccgccgcgc atcaaaaact
      481 gatccagacg atacagatcg tctaatttga cggaatccga aacgccattc atgatgatgg
      541 tctggatatc ggcggaaata tcgctgccga gagaaacgct gcgtacggtg tacaactgat
      601 ccaaaaagcg gttcagattt tgggtgctga tttcttcatt ttccggagaa atgataccaa
      661 cgtttttcag attggtcgcg gcgatttttt tcatcgtcgc cgtgatcatg tattccagac
      721 cttcggtgga ctggatcacg gggatgggtt cttttttatc gccgtagagc agtaccatgc
      781 ccatatagac attccgcatt tccatcttat cgttttcgac ggcttgcacg ggtacgggac
      841 cgatcccgag ttgctgcgct ttttctttgg ctttatcgtc ggcatccgga ttgacaaatt
      901 cgaaaacaaa ttggccgtcc gaaaacgcct gatattcttc cagaagatcc tgcacatagc
      961 ggcgcgtatt ggctaagggg ccgggcagat tatccgaaaa atagactttg acctgcatcg
     1021 gatctttgag tttgccgatg acttctttgc tggattcgga gagggaatac tgtttgttat
     1081 ccgtcgcatc ccatcgcacg aaaagattgc gggacacgat attggccatt acgacgatcg
     1141 ctgcgatgag caggacatag ttgatgaacg tgcttttatt ggtgattttg agcataagtt
     1201 ttatccgtta atacattcgt aaataatcaa cgccacttgc gcatttccag gagccgggtg
     1261 ctgaccaaaa ggaaaaacgc gatacccgat ccgaagtaga gcagattgcg ggagtccacc
     1321 acgccgcggg agataccttc gaggtgatga tccacgctca tgtactgcac aagtcctgcg
     1381 agaaatgccg ggacgaacgt gaccattttg ccgaaaaaga aaaagaaaac cgtgatgatc
     1441 agcagcgtga gtatgaatgc cgaaatctgg ttatcggtca cggtactgca aaaaatgccg
     1501 atcgcacagt agagcgcgcc gacaagcagg agcccgaagt atccgcacaa tacggcgccc
     1561 acatccacgt gtttgccgac ggcgatcagc gtaaaaaaat gcacgaggct aaatccgagc
     1621 gcggtcgcta cgagcgtaaa tgcagccaaa aactttccga tgatgatctg gctgtcggtc
     1681 accggcagag tgacaagaaa ctccagcgta cccgactggc gttcgcgggc cacaaggctc
     1741 atcgtgatcg cgggtacaaa aaacataaac acccacggca cgacttcaaa cagcgggcgc
     1801 agatcggatt ggttattgag aaaaaacgta ttgctaaaaa atatcgaggt catcaccgca
     1861 aatacggcga gcacgatata ggcgacgggg ctgttaaaaa acaccgccat ctctttgcgg
     1921 tagatcatgt gtgtattagg catggggttg gcctccttcg acggtgagtg tccggaaaat
     1981 atcttccagg ctgactttgt ggcggctcat ctccaaaata gcccactgct gtttgacggc
     2041 gtattcgaag atttcctttc gcggatcgga ctgattggga tattcgagac ggaccatcag
     2101 tgcgttgctg tcccgtgtta cttgggtgac ggtgataccg cccacggcgc cctgcagttt
     2161 gcgaacggct tcttcctgcg cgttgagcag ttccatcgtg acgacggtct tgcctttgaa
     2221 gccggacatc agttcggcgg tggtgccgtt ggcgacgatc tggcctttat tgatgataac
     2281 catgcggtcg gcggtggctt gcacttcctg caagatatga ctggacatga tcagggtttt
     2341 tttcttgccg aggtttttga tcagttcgcg gatttcgacg atctgattgg ggtcaagccc
     2401 ggtggacggt tcgtctaaga ttagaatttt gggatcgtga aagatcgcct gtgccagtcc
     2461 gacgcgctgt ttgtaacctt tggagagttc ggcgatggtt ttgtgaacga caccgcgcag
     2521 gccgcagagt tcgatcactt cgccgaggcg tttggtaaaa gcgctgcctt cgatctgacg
     2581 ggcggccgcg acgaatttca gaaaatcgta cacggtcatt tcgtaataca ggggattgag
     2641 ttccggcagg tacccgatct gcttgcgtat ctcaaggctg tgatcgagga tgttgcggtc
     2701 gttgataaaa acattacctg aagtgggcgc gagatacgac gtgatgatct tgagcgtcgt
     2761 ggatttgccg gcgccattgg gccccagaaa tccgaggatc tcgccgtcct gcacttcaaa
     2821 gctgatgcta ttgagcgcac gcacgctgcc gtaatctttg gtgagattct cgacacgtat
     2881 catagaaact ccgtttgggt taatgaatca tatactacac taaaaaacat aagactaaga
     2941 aacataagct atactaagag tatttctaaa acgcgggaaa gatagataac cgcaggggat
     3001 ttgtcaagac gggggtgcga ctgtgggtgt tttggggtga ttcgatgttc gggattgcat
     3061 tccgcttctt aaaatttttg gacagaaatt tgggcttcct ttcttgtgtt ttatggctca
     3121 atgcctaaaa tcaataatgg aaccggaggg ctatttcgtc cttgtctcat tacgttataa
     3181 acctctcctt catagtaagc tacaccacta ctcatctgcg attgcatttt ctttgttggt
     3241 aaaatttcaa ttccaagatt gattttaccg ccagaataga agagcatatg ttttacgaac
     3301 aaatcaaacg caacaaaagc atacttcccg aattgaactt ctacggctat tttgtcctta
     3361 acaaagtctg tttgattata actgtaaatt ggttcgttca ctccattctc aataagaaat
     3421 tctctttgct gctttgcagg cattaaaacg cttttttcca ttaactcgcg gtcaagtgtt
     3481 atataataat tgtatctgct ttctttccat ttgcgtttgg aaaaagcatc attaaatgct
     3541 ttattcaaat caataggaga tagtaacggg tttcctattt tccgtttctc cttgctaatc
     3601 ttggtcctag aggcgtcggc atcaatgccg acaataactt cttctatttc tttataaaga
     3661 tttttgtgat gtacaattaa atattcttcg ccattcaagt gggaatactt ctgtgcgatt
     3721 ttatttttat gtgcccaact gcattgcgac tattaaacat cgttgcgttg cactcttatg
     3781 ctgtaaatca ctactgagca agattatcaa gcaagttgct tttcaaaaag ctctacttca
     3841 tcgtagtcct tattgatttt ttttagaatt tctttctttc tcaataatgc agaattttca
     3901 tcattgaaaa tttcaggctc ctgaattagt ccatgatgaa tataaattaa aacccaaatt
     3961 cttttcattg tattaagatt taattgttct attaaaccat ccgaatacgt ctttgtattc
     4021 aagaaaatta caaatttctg aaagtccgct cactttaaaa gttggtctat taatttggcg
     4081 taagaacaaa gcccgtcgta aatcatttga aacgatactg aactttggct ttaaatttgg
     4141 ctcaactaag tgcaaatcat taaacctcaa aagacctgaa taaattgaag ttgaatgttc
     4201 aacttcaaac atagcagaca aatcacttga acctctcttg agccatagaa catcaacttg
     4261 cttaattacg tcaaatactt tctcatatct atctggtaaa tcatttttgc agataaattt
     4321 ttccgcaagt tgccaatcca gtttgtttct gtcaatatta ggtatccata tatcataccc
     4381 gtggtgaaca ccaattgagc caataagagt ttgaatttgc gaatggctaa aatcaggaat
     4441 agcaatgatt ttattcttgt caacaattaa atttaaaata ctccaaccga taaaagcttc
     4501 tacattaaat cttccggcgt tagcaatata taattcagtg ccatcttttt cttgataaat
     4561 ctgtgctttt atttgtccat cagaagctgg ttctaatcca gaaaagactt cttcaaattc
     4621 ttcataagga ataaacaatg gttcggtttg tgtatcccaa agaaaacaaa taacagaatt
     4681 aaatccttcc aactgcttta agtcatcttt tcttatccca taaaaagatt gatttctgct
     4741 atggactttt gaatatctga tataaattct acataagccg tctccaattt caaaaagaga
     4801 taaactgttc ggaagttttt tcaacttgcc aaaacgagct tctaaacttt taagaaaacc
     4861 aatttttaat tgattaggca ttttattatt ctatgctgtg acgttaagtt gagaagcatg
     4921 ggagattgaa gtagaaaatt cagtacgaac cgttgatttt acattaggaa tgttccacat
     4981 ggttttatcc cagtcttgtt caataatctc ccaaatgtcg ctaggatttt tacccaaagg
     5041 gttaccggat agttgcccct tttttggacc tttgaagtgc agctttcctg gatatttaga
     5101 tggaactcta accttatcaa gattaaaaat gtaatcgtct gttttgctaa accagaggat
     5161 tgtttcgtat cgaccactaa aacgattgct ggcgtgaagt ccatgcccga aatgccacac
     5221 tatacggttg cgaagtttta agccgtgttt cttaaatatt tgataataaa atatatcaag
     5281 cgggaatatt tctcctttgt cgacataatt tcctacctgc caacatagat ttccattttt
     5341 tgataatgtt cttacaagtt cagaaataat ttcttcctgc gtatcaagat aattttctat
     5401 acttgtttta gtctcatatg atttgcctat gttgtacggt ggtgaagtca aaatgaggtc
     5461 aaatttatta tcctcaattt tcttgagtat agttaaactg tctccgtttt taatagtgta
     5521 gtcaatgtcg ggactcaaat agtcaaatag aggttctttt attttcatct tctttatcat
     5581 aagttgtttt gcaattttaa tcttaagtgg ttaagtgcgt aaaaatcatt ataaaaatat
     5641 tagtgttctt gttgtaatat gtagtttcca actttgttta gtagcgttgt aataaataaa
     5701 aacaactctt tcctccatat aaaggaatac ctaagtttta cgttttttcc tttcttgcat
     5761 gttgcaaaaa tataatctca atcccatcaa aacaacacaa atcacagtct acaagtttta
     5821 gtgtgggtgt actataaaat gatatttctc acctcataat acaacacgat agaaccggag
     5881 ctgaatattt tggtgttggt gagtttgagt attctctttt cactgatttg tttaaacaga
     5941 ggtaaacttt ttatgcgctt ccgcagcggt tcgtttttag tgtttcaatt ttttgatgtg
     6001 ccttagcact tctctttcta cttggtcaaa gtcgggcagg tcttttattt gttcgcccat
     6061 agagcttgcc caccggcctt tgtattgagg caatcgatca gatagctttt gcgaaaaatc
     6121 ggtgttttgc agatttttgc atctgcactt tgtctcaaat tcgcggatat aaaaatccgg
     6181 ctccatgctt tgtttatcca gtagatacca gatatcgtat aagtcacggg cttgcattcg
     6241 ttgcatcacg gaacgcattt tttcaactaa cacttcctcc aaggggtagc aaagcaatga
     6301 gtgttcatgc aaatcagtgt agtcaacaaa tacatttttc atcacaggtt caaacaccaa
     6361 ttgctcactg cgtgaaatat caacttttac ccgtttatta tttccttgtc cgccaagcgg
     6421 accaatatag ctgatgtaaa aattaatgcc gccatcttca tgctcgttgt tgtcaatgat
     6481 atcaagcgga atattggctt catcttttat gaattcaaat acttctttaa accatgcgaa
     6541 aatttgttcg ttaggtaatt cagcattcag caatgtaaaa tctaagtcct cagaaaaacg
     6601 ataatcttca aaatagactt tctttaatac cgttccacct ttgaaaacga ttgtctttga
     6661 aagtggctca tgtttagaaa tgccaaacag aatccatgaa aggatgtaat ccttttcaat
     6721 ctgctggtct cgcacaccga ctgtatttgc tttttgctgt atttctccgg gtttgatcat
     6781 gtgtaaatgg ctgatttaat ggtttcaatt tccaagtttt gttgaatact ccaacgactt
     6841 attcgctttc ctgttttagg caattcggta tcaagcacca catacgatgc ggtttttagt
     6901 tgttttagtt cctgaacaat ttgggtattg atgtctaata tttctaaaag aaaacccaaa
     6961 cgtttgatta ccgcttgcga atcaaatttt ttggcgtagt caagaagtgt atcgtatttt
     7021 attttgtctt tagaagtgta gattgccctt gccacttcaa caaccccgcc tgcataatcg
     7081 ggtttgaata aacagtcaat aagagttttt tccaagtcgg aacacatgac tttattaaag
     7141 ctgtcaatcc agattttctt tgaaccgaaa aaatgtttct cgttgtgata gataaattga
     7201 aaaggaactt ctttgatttt tatttctgat ggtcggattt gttttgaaac aacgatttgt
     7261 tcttttaatg atggttgtgt aatgaggtta tgtatttgca aggctgaata atatccgata
     7321 taatgtttgg catcattcac caaatgctca gatatcaaat gccagtcggg cataaaggtt
     7381 tttgcatttt gttcgtatgg aatgatgtaa taaacaccat ctttcaaacg catcaacaaa
     7441 cctctctttg tcatgtcgct gagtagttct ctgactgcac ttgcattaga gtcgggcagg
     7501 gctttgtaag ccaaagaata gtcaaagcaa ttcctgctct gtccattaaa aaaagacaaa
     7561 agttcgcttg atcgtgttga tatgttttta ttattcatat ggcgtatgtc aggtttaagc
     7621 tgacatttat actataaaaa ataaatcaat agcgctgtaa tgtcaattta attgtgggtt
     7681 gataatcagc tagactcaag ccgacttatt tgatataatc atgacctatt ttttcaaaat
     7741 cttctttaaa acacttttgt accgggacgg acttactttg gtaagcacga tcgcggagca
     7801 gccgttgaac gcggcaaaac gtcgcacgga ttcaatgaaa gcttccgccc atatttcaaa
     7861 aggcagttct gtgttttcga tatgcaggtg gatcaactcc aaccgctgat cttttcggtg
     7921 cgctttgcag tccacgcggc cgataaaggt atcgccccat aagacaggca aacaaaaata
     7981 gccgtatagt ctttttttct gcggcgtgta gcattcgatc cgaaaatcaa aatcaaaaaa
     8041 atgtttgacg cggtcgcgat ggatgatggc attatcaaaa ggcgataata accggatcga
     8101 ggggtcgttt tttttgaatg ttttttcggt ccattccgaa agtgtaaata ccgacgggcg
     8161 acctttgata tctacgcact gcacagcgcc cttcgcaagc atggattgta aaatgtcctg
     8221 cacatttttt cgcaaacgtt catccgtttt aagatgggtg atttgtctga tcgtagtaaa
     8281 tccatatgcc cgcagatatg tcagaactaa atattcggcc cattccatcg gtgtcggttc
     8341 ggtcacatgc atggtttcgg gtaatacgcg ctcgcgcaga tcgtacgttt tttgcatgcc
     8401 gtcccgttcg ctgatcatca gatcgccctg cataaatagc ctttccagag ctagtttggt
     8461 aggtttccag ctccaccaac ttccggatct tttttttcgg ctttcaaaat ccctggcttt
     8521 tttgggacct tcggtgcgaa tcgtatccat cacatactgc atcactttgg gatcggcatt
     8581 gtaatacgga gacggaccgc gcttaaaacg catcatctgc ggcaacgcgt aacgaaaatc
     8641 tctaacggga agataggacg ccgcatgaaa ccaatattca aacactttgc gctcggctac
     8701 caattgatct aagtattctg ctttataatc cggaatccgc gtccatagcg tgtgatgatg
     8761 cgcacgttcg acgatggaca gagtgtcaat ctggatgtac ccgaggtgtt ccatagcgcg
     8821 gagaacagcc gctttacctt ttccaaacgg cgccggtttc gtcagcccct gactttccaa
     8881 tgtgatacgg cggagatggc gcgatgcctc ggcgtccttc atgatcggct ttcttgattt
     8941 attataagta gttggcacgc aatgttgccg aaaaaacaga gcaaaaaaat atgacgttca
     9001 aaacgtacga aaagaaaacc ccgacgaatg ctacggattt ttaatgtaat ttacaggctt
     9061 ttttggtcgg ctatcgtagg acgtacggcg agacgatgtt atgtcgtttc atacaacctt
     9121 ataaaaaggg cgttaatgga tatcaaaaat aaaaaaggga tgaatccgag cgactcatcc
     9181 cttacgttaa aaatgaaacg cgattaaccg ttcatggact ccaggaaatg cttattggag
     9241 cgtgtgccgc gcattttgtc gagcaaatat tccatcgctt cgaccggcgt cttatcactc
     9301 atcagtttac gcagaatcca tacgcggctt tgttcaaact gcgtaaggag aagttcttct
     9361 ttacgggtac cggagcgatt gatgtccatc gcaggataga cacggcgatc ggacaaacgg
     9421 cgatcaagga tgatttccat attacccgta cctttgaatt cttcaaagat aacatcatcc
     9481 atacgcgagc cggtttcgat caaagcggtc gcgatgatcg tcaaactgcc gccttcttcg
     9541 actttacgag ccgcgccgaa aaagcgtttg gggcgatgaa gggcgttaga atccacaccg
     9601 ccggaaagaa ttttaccgga atgcggtacg accgtattat gtgcacgggc aaggcgtgtg
     9661 atcgaatcca aaagaatcac gacatcgtgt ccgcattcga ccatacgttt ggctttttcg
     9721 agaacgatat ccgacacttg tacgtgacgc tcgggcggtt cgtcgaaggt ggaacttaat
     9781 acttccgctt ttacagagcg ttccatatcg gtcacttctt cggggcgctc gtcgatcagc
     9841 agtacgatca ttttgacttc gggatgattt tccgtaatgc tattggcgat atttttcaaa
     9901 agaatcgttt taccggtacg agggggtgcg acgatcagac cgcgctggcc tttaccgatg
     9961 ggcgtcatca gattcatgat gcgcatcgag tagttattgg cgttcttcgt ttccagttca
    10021 agacgttctt taggatagat cggcgtgagg ttatcaaaca gaattttatt tttggcttct
    10081 tcgggatttt cgaagttgac cgcttcgaca cgcaggagcg cgtaaaaacg ttcgctttcc
    10141 ttcggagggc gaatctgtcc gctgatcaca tcgccggtgc gtaagccgaa ttttttgatt
    10201 tgtgacgggg agatgtagat atcatccggg ctcggcaggt agttatagtt ttgcgaacgg
    10261 aggaaaccat agccgtcggg tagtatttcc aaaacgcctt cgctgaaaat aaagccatcg
    10321 cgtttggttt gttcttcgag gatcgtaaaa atcagatcct gttttttcat cccggtaaag
    10381 ctcagcatgc cgagatcttt ggcgatatcg ttaagttccg agattttgag gtctttgagt
    10441 ttggctagat tcagcgatgt tttttcccgg ggtgcgggag cttcttcttc gacatcggcc
    10501 ggtaaaccgg ctttcgaatc ggctttttta tctctgggtg tacgcgcacc gtcgtgtttg
    10561 cgtgtggttt taggttcagc ggtttccgct acgggttctg ccgcttcagt tttgatttct
    10621 ttttcttcgc tcatgttggg gtaagattaa gtgaaaaacg gttataaaga gaaaattaac
    10681 aattaagaat gattcagttt tatagaggca acgatattca acgaaaattt gtccgattta
    10741 gcgccgaaca gaaaattaag tgttggaatt taaggatgtg aacctttgca gatcgaagtc
    10801 tctgtagcgt accccaaagt atatgaatca cccaatattg tcaagttttt ttaagtttga
    10861 cggaattttc ttccttaatc gctgaaaact ggtaatccgc aaaccccaaa gcaaacaata
    10921 cggccgtaag atcgttgtaa cgaatgcgat tcatcacggt ttgttcgacg atgggtttcg
    10981 catgataagc tatcccgtca ccggccgtga tcaacattgg taagtcgttg gcgccatcac
    11041 ccaaagctat agccaggctc gtatcaaacc gaagtgttgc acagtgttgg atgagaacat
    11101 gccgtttagt ttctttgtca ataataggtc cgctgacttc accggtcaaa acgccgtcac
    11161 gtatctctaa ggtattggca tgatggtgat gaaatccgag ttcctccgaa agacgagcgg
    11221 tgaaatatgt aaatccgccg gacaccatca cgaggtgtaa accttgctcg cgcagtgtcg
    11281 tactgagcgt gcgtgcaccg ggcgtgagcc gaatcgcatt cgcataaact tcttccaatg
    11341 cggaggcagg caatccgcga agtaatgcga cacgtttgcg caacgcttca tcaaacggga
    11401 tagcgccatg catagccgat gcggtcatct ccgacacggt cttttctacg ccggccatgc
    11461 gtgctaattc gtcaatgcat tcgttttgta tcaatgtcga atcaacgtcg gtcaccaata
    11521 tttttttgga cctggtcgct aaatccaaaa tgaaataatc gatggcataa cgttgaagaa
    11581 agtccggtaa tgtcttcgcg atatgggcat gcatgcgggc tttatcggta cataccgaat
    11641 aaatttcgat ggcccgaaat tccgataacc atcggcgatc atgcacggct gtaatctcag
    11701 ccagcgaact gagtacgtgc acccatgccg actccggcaa gggttgcaag ccgacaaata
    11761 cgatgactgt tttattatcc cgtgaaaaca tggctggtat atatattccc gaagaaccta
    11821 tccatttaat gtaaaacggt ttttggaata ttcatatatg tatttttaaa aaacctttga
    11881 cgtgaaaagt tttttcggag aaacctactt gcatcggaca gtcgagttag acatcaggga
    11941 tgttccaaaa tgtccggcag agaatttcaa aattcccgat ttttcttttc attaccgatg
    12001 tcagttacta tatttcgcca gcacgtccca agcggttttc aaggagatgt tttatggagc
    12061 aggataataa accaaccccc aaagaggatt tggccggcga atatgccgat ttggtcgaaa
    12121 cgcctctttt ttcacgattc aaagaatggt ggcgcgccaa aaaggttagc ctcgatttat
    12181 tgggtgtcga gaaaatcatc ggtgtggaca acaggcaata taaagccgcc gtcaaacaat
    12241 ataccaatct gcaggaagaa attgagcttc agtacaaaga agtcgttaag aatcctgatg
    12301 tgactccgga agaagtagaa acggcaaaag ccgaacgtga ggcgcaaatc ctcgacgcta
    12361 aaaaagattt tgtgtatcac atgccggaag attaccggac ttggttcaaa aaacgaaaag
    12421 tgtttaactc ctggcgtcat gcgccggaag cttggggatt tgaagaatcc cgttattggg
    12481 gaagtcgctc cggcttgacc gtgcgtgaaa aacaaattct catcggcgga ttgatcatcg
    12541 gcagtgtggt gatcgggttt atcgtgtacc gaattgcgac ctatacaccg ccgacgcaag
    12601 ctgtggtaag ggagctgaag gttgaaaaag attcgacgat gaataatgtg gattcgctga
    12661 tgcgcgtcaa agaacaacaa cgtcagcagc gcacgatcac ggaacgcaat aacatcatca
    12721 tcaatgaaat caacaacgga atgtacacga cccaatcgtg gaatcctcag gacgaaattc
    12781 gcctgatcat gtctctgtat tatcagtata cattgtatca aaccatagct ccggcggata
    12841 ttactaacga gggtctcaaa tcggtttcgg gattgatata tcaaaacaac ccggatgtat
    12901 ttaccaaatt cgatgcgggc ggtatcccca ccgtttggaa aagccctaaa aaaatcaaac
    12961 tcaattggga tgaaattcgt accggcgtag agcgcgacaa aaaagtaagg gcaattcgcc
    13021 gcaaataagt tagaattaat tcatattaag atttatatac aggaagaagg atgaaaccgt
    13081 tttctctcgg aaaaaaacgt attttttatt ataccgaaga cgcccggtcg gtacgcgatc
    13141 agcttgaggg gcgttcgttt acatacacgg gccagccgtt ggtttttggc gtgagtaccg
    13201 atacgatcat caatggtgcg gcatgtacgt tgggttatac ggagccgata ctcggcccgc
    13261 attttttggt cggcaaaggt gatgtcatcc atgaaggaga cgttcaaaaa ggaaattttt
    13321 ctgtcgtggt cggcggcgaa agttatggca gcggttcctc gcgtgaagtg gccgtggtcg
    13381 cgcatcgcgg cgccggtatc gaactggtga ttgccaaaag cctcgaacgt attttccgtg
    13441 aaaacatgac atttgaccgt ctgtatttta ccaatgatat gacggtgatg gataagttgc
    13501 ttgccggtga aacggtagat cttgatgtac ttatggaaaa gcagttgtcg ccgttgtata
    13561 aatctgtatt ttttggcggc ggacttttgg agtttggaaa aaaagttatc gccggaaaag
    13621 cggagttgcc ttataaagcc gtcaggcgtt ccgtaaaacc gatgactatt gctgaaaaaa
    13681 tattggcgcg caaagctgta gccacggatg ctcccggagg acagggtttg aattattcgg
    13741 aacccggtga acaacttttc gtcaaagcgg attttctcgg tattcatgaa tacaccggcg
    13801 gtcatgtgcg cagtttgtac gaaaaagcgt ttggtcacaa tccgaccgat aaaggcgtga
    13861 ctattcgcgg atttaacgat cactttgttt tgatcgggca tgacgatgtg cccggcgatg
    13921 taaaatccaa gcgtttggct tcggcgcagg cgctcgcgca agaattgatc ggattttgca
    13981 aagagcacaa tcttccgatg cacggtccgg ggggcaatct tcccgccggt gtctgtcacc
    14041 gtttggttgt tgaaagttat gccaaacccg gcgatgtcat agcgcttacg gattcgcaca
    14101 caccgacggc gggcgtgatg aattgttttg catacggact tggttcgaca gccatcgcgt
    14161 tgggattggg caccggcatg atcccggtaa ccgtacctaa aactgtacgc atctggatcg
    14221 aaggtgaaga taaactcaat ctggtaacac ctaaagatat tatccttcac atcatcggtg
    14281 atccgtattt ccgcgagatg cagtggaaaa aagaaccggg tgataccgtg atcatgcaat
    14341 tcggcgggcc cgcactggaa cgctttaccg tggatgaatt atccgttttg acgaatatga
    14401 cggtcgaagg aagcgcaacc accggtgtcg tcgagccttg tacgccggtc attcgtttcc
    14461 tgatcgaaaa acgcggcatg acggaatccg atgtgcgatc tatgcttgtt tatcccgatg
    14521 ataaggcgac gtacgaatcg gtgtataaag tcaatctcgc ggatgtaccg gttacggtgg
    14581 ccacacccgg cgatagtcgt aatcgcaaac cgattacggc cgttgccaaa acgcgtattg
    14641 acaatgtggt tattgtttct tgtaccggtg catctttgga agatctcaaa caagtggcta
    14701 tggccattcg tggtcacaaa aagcttgatc gtattcacat ggtcgtgacg cccagcagtc
    14761 aaaaagtcta tgatgatgcc aagcaacttg gtatgatcaa gatgtttcag gactttggcg
    14821 ccgtagtaac gaaaccggga tgcggatctt gtatcggcaa cgggccggga atttctacta
    14881 aagacacgat cactgcatct acgagcaatc gtaatttcag cggtcgtatg ggcggaaacg
    14941 gggatgtatt tctcgtttct ccgttcgttg caggggtggc ggcggtgacc ggcgaattga
    15001 cagatccgcg cggatggcaa gtagtgaatg cgtaagtaaa gcatcgaaag ttttcatggt
    15061 tcgctgatca tactcaattc acattttcag attttatttc aaatcattcc tcaaaggaac
    15121 agatatgttc aaaggacatc cgcgcggcct caaagtcttg ttcttctcgg aaatgtggga
    15181 acgctttggt atctatacga tgcttgcgat tttcacattg tatatgggaa attacttcgg
    15241 ttggcctgac accaaaacgg gacagattta cggaatattc cttgcactga cttatttttc
    15301 gccggtactt ggcggatacc ttgccgataa gttttggggc tttcctaaaa ccattatggt
    15361 gggcgctatt gcgaccggta tcggttatgc tatattggct attcccaaac cgtccgaacc
    15421 gcttttttat ctcggtttat ttgtcgtggc gatgagcaat ggtcttttca aagcgaatat
    15481 ctccgttctc gtcggcaatt tgtatgaaaa aaacagccct ctcaaagatg ccggttataa
    15541 cattttttat atgggtatca atgtcggcgc ttttttagct ccgattgcgg ctacttttct
    15601 tagaaattcg atcggcacgg aatacggtta caatgcggga tttggcgccg ccgccgccgg
    15661 catgcttttt tcttttatca catttcgtct tggccaggct tcctataatg cgtctgctgc
    15721 cgctgtagcc gcaaacggca gtacgacgat ggcgaccgag gagcgtgttt cgcccaaaaa
    15781 agaaaaagat cgtgtgatcg ctttgatcat cgtatatctg atcgtcgttt ttttctggat
    15841 gtcttttcac caaaacggat ttgcactgac cttatttgcc gaacgctcca cgcgaccgat
    15901 ggatttgcct gaaggcttgc gtagcgtgct ttatgtgatt ttgggtatat ccgatccggc
    15961 gaacgcatcg gaagctgaat tttatcaggt attcaacccg atgttcatac tcatgatgac
    16021 cccgctggtt gtcatgttct gggggtggct caataaacgt gggctggagc ccgcaacgcc
    16081 ggccaaaatc ggaatcggca tgatgctctg cgggttgggt tttgccatca tgatttttgc
    16141 ttcgatgggc ggtggtgatg ccgataataa atccatgtct ccgctgtggc ttatttcatc
    16201 gtattatgta caaacattag ctgaattatg cctcagtccg atggggcttt cgtatgccag
    16261 caagatggcc ccgtaccgct ggcgcgggtt acttatgggc ggttggttcg ccgcgacggc
    16321 gataggtaat tattttgccg gatttctcgg ttcgttttac agtacctggt ctcacacgca
    16381 gttttatact gtcttgactg tatgttgttt cttctcggcc gtgatgattg ttttcttttt
    16441 gaaaaaactt aaacatgcgg cagagcctga cgaaccgatc gaaaatatta cgggcgaagt
    16501 ttggggcacc aagccatcct aaattctgct tgacagcgcg gtcctgcttc gatatattga
    16561 cccgctttta aaaaattcgt tctttcacat atatccttct tatgaagagt ggccgagtga
    16621 tcaggcacga tgaagccaca gcaaccgtac gccgccgcgc gtagtcaagg tgctaatacc
    16681 tgccgaacgc aatgtaagcg gtcggggcaa tgagatggaa gcaacacaga tttctttcaa
    16741 aacctctccc gacttcggtt gaggtttttt tattgttcat tttaataacc tatttattaa
    16801 tacaggagta attcatgtca cattatttgt ttacatcgga atcggtatcc gaaggacatc
    16861 ccgacaaaat agcggatcag atttccgatg cgctcgtaga tcactttatc gcatttgatc
    16921 cgtattccaa agtcgcgtgc gaaaccttgg ttacgaccgg tcaggtcgta ttggccggcg
    16981 aagtcaaatc cagtacgtat cttgacgtgc aggaaattac gcgcggcgtt attcgtaaga
    17041 tcggttacac caagagtgaa tatatgtttg aagcgcactc ctgtggtatt ctttccgcga
    17101 ttcacgaaca gtcgcaggat atcaatcgtg gtgttgacaa aaagaaaaaa gaagaacaag
    17161 gcgccggcga tcaaggtatg atgttcggtt atgcggtcaa tgaaacagcg gactacatgc
    17221 cgctgacgct ggatctcgcc cataagttat tacaagaact ggctgtgctt cgccgggaga
    17281 acaaggacat caaatatctt cgtccggatg ccaaatccca ggtgacgctg gaatacgatg
    17341 aaaaccgtaa gcccgtgcgc attgatacga ttgttatttc cacgcagcac gatgatttcg
    17401 gatcggacag cgcgatgcag gataaaatcc gcaaagatat gatcaatatc ctgatccccc
    17461 gtgttatgaa gcgttatccc aagtacaaac atctttttaa taaccagatc aaatatttca
    17521 tcaatccgac gggcaaattt gtgatcggcg gtccgcacgg tgatacggga ttgacaggtc
    17581 gtaaaatcat cgtggacacc tatggcggac gtggggcaca cggtggcggc gcattcagcg
    17641 gtaaagatcc ttccaaagtg gatcgttcgg cagcgtatgc gacgcgacat atcgctaaaa
    17701 atcttgttgc cgccggtgtg gttgatgaag tactggtgca ggtttcgtat gctatcggtg
    17761 tagccaaacc gatgggtatt tttgtcaaca ccttcggtac aggcaaagtc ggtatgagcg
    17821 atgccgatat tgctaaaaaa gtcgaatcca tttttgatat gcgcccttat tttatcgaaa
    17881 aacgcctcaa actgcgcacg ccgatgtatt cggagtcggc tgcgtacgga catatgggtc
    17941 gtaagaacga atgggttaca aaaacattca cctccggtga cggcatcaaa aaaacggtga
    18001 aggtcgaact ttttacgtgg gaaaaattag actacgtagg aaaagtgaaa gccgcgttta
    18061 gaattaaata atacgtttgg ttattattac atataagggc tgctttaagg gtttggtcga
    18121 agcgaaaaaa cttcccggaa tccgctaaag cagcccttat gaattttggc ttatgattat
    18181 cattattgat aactacgatt cctttactta caacctctat caatacgtgc gcgaattagc
    18241 ggcaccgatg tcggatgtgc gagtatatcg ccacgatgaa atcacgattc ctcaacttga
    18301 ggcattacga cccgatcgca tcatcatatc gccgggaccc ggttctccgc atgatgccgg
    18361 tatttccaat gacgtgatcc gatatgccgc agataccgta cctctgctcg gtgtatgcct
    18421 tggtcatcag tgcctggctc aagtgtatgg cgcccgcatc ctacgcgcaa acgaacctaa
    18481 gcatggtaaa gtatcggtca taaaacacga cggtagaggt gtgtttgcgg gtattcctaa
    18541 tcctcttcgg gttacacggt atcattctct gatcgttgat ccgatgagtc tgaatcaaaa
    18601 gatagaaata tcagcacaga cggatggcgg tgaaatcatg ggcttgcgta tacagggtac
    18661 cagagcggaa agcgtgcagt ttcatcccga atccgtgatg accgaacacg gaaaaaaaat
    18721 gataaataat tttttaaaaa tgtgattttt tgcttgccaa ctaagggtaa tttctatata
    18781 attgcacgct tcaaatgaag caggcgagct agtagctcag tcggtagagc agcgcccttt
    18841 taaggcgaag gtcgtaggtt cgagccctac ctggctcaca aaacaaaatc ccgatcacat
    18901 agtgaccggg atttttgttt tcaatatttg gtatttgtat tattcttcgt aacccacctg
    18961 attacgtcct tgcgatttgg cgacgtacag ttgttgatcc gtgcgctcca gtacctcttc
    19021 gagcgttgcg ccgtctttgg gaaaagacgc aacgccgagt gaaatcgtaa cacggtcggt
    19081 atgaaaggtc gaatgcggat tctgagacac aaaaagatga ttgatctgaa ccgattgttc
    19141 atcgatgatt ttgcgtagtt tttcggcaac gatcgctgca cccttgacat cggtttccgg
    19201 taagatcact ataaactcct caccgccata gcgggccaac acatcatgat cgcgtaatcc
    19261 ttttccaaga ttttttgcaa cccataccag tacttcatct ccgactttgt ggccgtaggt
    19321 atcgttaaat tttttaaagt gatcaatgtc catcaaaata aatgacagga tgtgcttgta
    19381 ccgtttggca cggttaaatt cacgctgtga acggtcgttg aaatagcgcc gattgtaaat
    19441 ccctgtaaga ccgtctttga tggagatttc aaaagtttcc tgatacaaca aagcgcgctc
    19501 aaccgccaca ccgatttcat tggtgataag gttcaaaaat tctttttcac cgtcggatag
    19561 actatgctcg gcgaaatagc aaaccacttt gcttgatttt ttgtgaaacg tcaacggcag
    19621 tgcgacgatg ctgccttcgg ttttcatcgg aaaatatttt aacaaatcct ttcgttcgtt
    19681 aacggaaatc acgtaagact ctttttccgt tgaaaacact ttttccgcta cttctttttc
    19741 cgcatcaagc acatgcgcaa gagaacgatc tttggtgtgg gtgtgaaaaa gtacatttac
    19801 cgaacccgaa tcggttctct cgatcagggc gtaatgccgg acaaacgaaa tatccgacag
    19861 catgttttgg atgatattat aaagtttggt cgtatcgtac gtcgaattga gcatagccgc
    19921 tattttataa agcaggcttg tttggtgcag gcgtttgtgg agaagctgat tttcatcctg
    19981 gacgcgtata tacgatgctt ccagttctga aactttggat ttcagttcct cgatcaaaag
    20041 cgtttccgaa gcatccattt gtgtcggtga tggttcggtg tttgtggggc gatttttcat
    20101 agatttttgt gttgatatgg aagccgaagg agatgacttt ctacgcatac aacattacgc
    20161 cgtttttttg acggtcgaat cttccggttc cgcaaacgtc atgcgcggtt tgccgcccga
    20221 tacgacacaa tctttgtgaa tagtaactga ttttaaatgc gaaatactgg gtatctgata
    20281 catgatgtcg gtcataaatt tttccataat agcacgaagc ccgcgcgcgc cggtctttcg
    20341 tctgaccgca agtttgacga cttccttcaa tgctttttca tcaaactcca gttcgacgtg
    20401 ttccagtgca aaaagttttt tatactgttt gaccaacgca ttcttgggtt gaacaagaat
    20461 agaatagagc gccgattcgg acagttcttc gagcggactg attacaggta aacgccctat
    20521 caattcggga atgatgccga atcccagcag atcgtccggt tctaccaatt tgagtacttc
    20581 gctcacggac tgttcggctt ttttgtccgc tgaagccgta aatccgatat tctttttgcc
    20641 gacacgcgtt tgaataattt tttcgattcc ttcgaaagcg ccgccgcaga tgaaaagaat
    20701 atttttggta ttgatactga tgaggttttg ttcaggatgc ttgcgaccgc ctttgggagg
    20761 aacattggcc acagtgcctt ccaggatttt cagaagagcc tgctgaacgc cttcaccgga
    20821 aacgtcgcgt gtgatcgaag ggttgccgcc tttgcgtgca attttatcaa tttcgtcaat
    20881 atagataatg ccgcgttctg ccgcctggac gtcatagttg gcagcctgca ggagacgcac
    20941 gagcacattt tcaacgtctt cgccgacata gccggcttca gtcaacgtcg tggcatcggc
    21001 tatcgtaaac ggtacgttca aaaatttggc taacgtttgc gcaagtaagg tcttaccggt
    21061 gcctgtcggt ccgatcagga gaatgttgtt tttttcgagt tcaacatcgt tttcagaatc
    21121 ctggagcgca ttgattcgtt tgtagtgatt atagacagca acggataacg atttttttgc
    21181 aaaatcctgg ccgacaatgt attcatctaa cttcagtttc agtttttcgg gcgtgatgtc
    21241 ggtcagcggt aatgaaacgt cgcgtgtcgt atgcgagcga atcatatcga acgcattacg
    21301 tacgcattca ttgcagatgt ggacgccgtt aggtcccgaa ataatgctgt ctacttcttc
    21361 ggctgattta ccgcaaaagg agcaaaatac agtacgtgcc atggactaac tttcaggtta
    21421 tggctttaga ataagaaata gggttcgcgt ttgcaacaat ataaaccgat aagatcccgg
    21481 cggagaggat gccggaccgg ataatgttat atacacgcaa ttattttttg cgtacaagca
    21541 cttcgtctat gatgccgtat gctttggctt cgtcggaaga cataaagaaa ttgcgatcaa
    21601 tatctttttc aaggcgttcc gcgttttggc cggtgcgctg catgatgatt ttgttcaatt
    21661 gatctttcat cttcacgatt tcctgcatat agatttcgat atcggaagat tgtccctgcg
    21721 tttctccgag cggctgatgc agcatgatcc gcgaatgcgg taaggcataa cgttttcctt
    21781 tggtgccggc catcaggagg atagcgccca tactggccgc catgccggta caggtggtct
    21841 gaatttccgg tttgataaac tccatcgtat cgtaaatcgc aagtccgtcg gtcacgctgc
    21901 cgccggggct attgatgtac atgatgatgt ctttttccgg atcggtcgcc gcaagaaaga
    21961 gtagctgcgc aatgacgacg ttggaaatct cataattaat cggcgtaccg agaaatataa
    22021 tacgctccat cagcaggcgc gaataaatat catagacgcg ctccatctga cctttttgtt
    22081 cgatcacata aggcacgacg gaaccggaga tggatcgcaa gctgtcgtcg ctgaggcggt
    22141 taaattcact tttgacaaat gattcgtatg gattcataga cgtcctggtt tcggtcatca
    22201 aattcaaggt ttaaagtgca aaaactctga acaaaaagca aagtcagagt ttttacaaat
    22261 ttaaaataca gatattagct atgtcgtgta caatatcagc ttattttttc tcttccgaga
    22321 gcatgtcggg tacttcttcg actttagctg agtcggcaat cagtttggct gctttttctt
    22381 caagcaaata atcctcgaag ttgcgttgtt ccgtttccgt atggaagtga acgttgatct
    22441 gttccaaagg tactttattt tgtttggctg ttttttcgcg ataggcctgg atgtcggcat
    22501 cttcaacctt caggttttcc tgttcgatga ttttgcgttt agccaatgtc cattttactt
    22561 cgcggatggc gcttgggcga taacgttcgg cgatgagttc gtctttgacg ggctggcctt
    22621 tgaggcgttc tttgatcatg cgtccgatgc gctgaagcga attatctacc agcggttccg
    22681 gagcagggat ttcgttttgt ttgataattt cgtcaatcaa agcttcacga aatttttgtt
    22741 cggactcttg ctcccagcgt tcgctgagat ttttgtgcat atcggctttg agtaattcga
    22801 atgtctcaaa tccgaggtct ttggcgaatt cgtcatccag agccggcagt tgcatttctt
    22861 cgattttgcg aacggtcacc tgatattttt ctttttcgat cgttgcgatg tcttcgcctt
    22921 cctgcggagg acgtttggct acatcaagga tgcgcatgtc gcctgttttg gcgccgataa
    22981 ggccgtcaat aaaatcctgt cccacgaatt ggcgagtgag cggaatgcga cgatcttcat
    23041 attttttgcc gatgagcggc gtaccgcttt catccagcac ctgaatatcc accgttgcga
    23101 agttacccgc agcaatcaca tcggtttctt tggtcgtgga atagctttcg cgcaaacgat
    23161 caaaagcgcc ggtcacttct tcgtctgtta ttttgtgtac ttcgcgtttt aagttgaggc
    23221 ctttgtaatt ttggacgtcg tacttgggca tgatctcgac ggtcacgtca aacttcagcg
    23281 gttgaccttt tttgtagtcc atgttttcga ttttgccgat cgcgatggct tcggtcttag
    23341 tcgcttcgag cgcttcgcgg taaaaatctt ccacgaccac ttccagcgct tccgcttcga
    23401 tcatattgcc aaattttttc tgaatcacat ctttgggggc tttgccttta cgaaaacctt
    23461 cgatctgggc ttcttcacgg tacatcgcca aagcctcttc aaagtatggc aacatcgcat
    23521 cgtagggcac ttctaccgct aattttcggg tagcattatc ttcgtgaatc acactcgctt
    23581 gcaaaggaat tctcctgtgt tataaatatt tattttaatg ttttcaaaaa atggtgcggg
    23641 atgagggact tgaacccaca cggtaaaacc accagatcct aagtctggcg cgtctgccaa
    23701 ttccgccaat cccgcatata taggccaaat gcggcgcaat atagccaacg agccctgcaa
    23761 tagcaagttt ttatttgatt ttaaagtgtc ggcatcccta tattgaccgc attgtaaaaa
    23821 tcggagaatc aaaaacttgg gatcatccaa aaagacaaaa aaagcgacac ctaaagccgg
    23881 aaaatcaagc aaaccggcta agaaaaaagc agcgcccaag acgggcaaaa ctttaaaaaa
    23941 atccggcaca gcggtcaata aaaccaaaaa atccgtcaaa accgttaaaa aagcggcgaa
    24001 aacagctgtt tccaaaaaaa ccaaaacggt taaaaaagta gtaaccaaaa aaaccaaacc
    24061 cgtcaaaaaa gatgcgctca tcgcacagga cgaaatgatg gatgaggatg tggaccggtt
    24121 gacgacagaa gaaattgatg cggaattggc ggcttatgaa agtgaaaaag gcaaaccgcg
    24181 tgaccctaaa accggaaaaa ccttaccgga gtccgaggcc atgcagctgt gtcgccgtgt
    24241 aacggaatac atgtttgaga aaaaggctga agacgtcgtc atcgccgatt tagaaggatt
    24301 gtccagcgcc gccgattttt ttgtgatatg tacggcgaca tccgacatgc atgccaaagc
    24361 cattgcggat aatgtggaag atcgcctcgc tcaggaaggc gctaaactcc atcacaaaga
    24421 aggtcaccaa tcgctgaaat gggttctttt ggattatgtg gacgttatcg tccatgtttt
    24481 ccaaaaagat gcgcgttcgt tttatgatct cgaacgtcta tggggcgacg ccaaatttac
    24541 caaagtgcgg gacactgccg ggcgcgcata atgttttttc gattgtcttc ggtacgtata
    24601 catcgtttgt aagttctttt tatttagacg gatttttccc gccgaactgc atatattttt
    24661 tcgtttcgta gatttataca gcgagtactg cgtatatcac ttgtatcgga tgctcaggtt
    24721 atgacaaaag aaatgtggct caacctgcct gtcaaagaca taaaaaaagc cagggcattt
    24781 tatagtcatc tcggttttgc gttcaattca gaacgcgata ccgaacatac ggcatgcatg
    24841 ctggtcggcg atcaagatgc cggcaaagaa acgatagtca tgttgttttc ggagcatgtc
    24901 ttccaaagct ttacccatca tccgattgcg gatacgcgcc acggttcgca agttcttttt
    24961 tcgatcgatg cggagagtcg cgaggaagtg aatcgccttg cggcaagagc ggaagcggcc
    25021 ggcggaaaag tatttgccaa accggcagaa aatacagcgt ggatgtatgg atgcgggttc
    25081 tgcgatccgg acggacaccg gtggaatgtt ttattcatgg acatggataa aatggaaaaa
    25141 ttttgacctt atgaattata atgactatat ccttgccatc ataccggtgt ttgtcgctat
    25201 tgatgtgatc ggttttatgc cgctttatat cacgttgact gacggcatac cggagcccgt
    25261 caaatcgcgc gtactacgtc aatctattct gacggctttt ttggttacga tgactttttt
    25321 ggcgctgggc aaggcgatat ttgcgtttct cggcatttcg atcagcgact tcaaaatcgc
    25381 cggcggtatt attctttttg caattgccat caatgatctc atttcctcat cggacgatcg
    25441 catcagcaac gaagtccgcg atgaaacaac cgaaatacat atcaatcaga atccgacgat
    25501 cggcatcgtg cccatcggaa taccgctgat cgtaggcccg gctttactca caaccatgat
    25561 gatcacgatc gacagctacg gtatgggacc gacgattttt agtgtgatga tcaatctgct
    25621 catcgtttac ggcgcgctgc atttttcacc gtatgttcta cgtattacgg ggcggagcgg
    25681 cgccaaaggc atcgctaaag tatttaccat cttattagcc gcgatcggtg tgatgatggt
    25741 gcgtgtcggg atcacggaaa ttattaacgg aaaagtataa tgcgcaatca ggacgaagaa
    25801 ccgctcaatg aatgggattt gccggaagag ccggagtcgg atgaggaaga cccggaagcc
    25861 gcgcatatcg cgctgaccaa agagatgtac tataccgcga tcattgagaa tatctacaat
    25921 gaagataccg aaaagcgccg ggaagctatc gacctgatct gtcattttga atgcgacccg
    25981 cgattagaag agccgttgat gaagttggtt cgttcgacgg atgcgcgatt tgtgctgtcg
    26041 ggtattgtcg gtcttggtaa ctggcgccat actgacggcg ctgaagtgct gatcgaaatg
    26101 ataaaccatc cgacaccggg cgtactttgg gatatggaga tgctgcagga agcgctgatc
    26161 gcactggggc gcatcggcgg acaagagccg cttgcgtttt tgaaaacgta tgcccatcag
    26221 cgataccatg atcacgacgg cggcgaagat actttgggta tggcggctat cgaagccatc
    26281 actgaaatcg caacgcacgg ccagcacggc gcggtcgaat ttctgatcga agggtgccgc
    26341 agtcttgcat ggaatatgcg tgaagcctgc gccgacagct tcagcgtagt gtatgccggt
    26401 aaagaacaat tgcctaaaaa tgtgtacgag acattagtca ctctcactaa ggatgaaaat
    26461 aaaaatgttc gtattgcggc ctatatgagt ttagacgaca ttgtagggct tgacgaaaaa
    26521 aacaaacaga tactcgccga cgcacggcat aaacaggttt ttggaaaata atcaaaccga
    26581 ggcgctatga atctttttct tcttgtcatt acggtgtacg tactggtcct catcggtatc
    26641 ggtgtttaca aaagttttca aatcaaaagc caagacgatt tcatggtagc cggtcgcaat
    26701 gtttccatgt ggtttttggt cggtacgctc gtttgcacat gggtcggatc ggggacgctt
    26761 tttggaagcg ccgggttgtc gtttcgtgac ggtttttcgg cattatggat gtcggccggc
    26821 gcatgggtag ggatcgtgtg cgtatatttc cttgcgggtc gcgttcgcaa aatcgcggaa
    26881 tataccgtac ctgacatttt ggaaaagcgg tacaatgcga cggcgcgtat cctcggaacg
    26941 atcaccatcg tcattgctta tatgtccatc gctggatatc aatttaaagg cggcggacgt
    27001 ttgctcaaca tcataacccg tacggacccg cataatgcgg caacgggcct tgatccgttt
    27061 gtcggcgcct tgatcacgtg cggtgtcgtc gtactgttta cattattggc cggtatggtg
    27121 tctatcatcg cggctgatct tgtcaatgga ttgatcatca cgatttcggt tatcatcggt
    27181 gttcctttgc tgttgtttgg cagtgatatc ggcagttgga attttgtaac tgaaacgctg
    27241 cccaacgatc attttaccgc attgggaacg cataatgcat ggtgggtgat cggtatcttt
    27301 ttcccgacat tttttctgct gatgggtgaa agcagtatct atcaaaagtt ttttgcggcg
    27361 aaggatgaag cgacggcaaa aaaagcggtc atcggcatgg tcatcggtgt tatttttgta
    27421 gagggtatgt tgtgccttct gtctattatc ggcagcgcca aacattggtc caatcctata
    27481 ttcaaaacgg cggacggcgg aattgatcct tcgatgtcgg aaacggttgt gcttcatata
    27541 gcgcgatttg atttgccgga ggttgccggt gcgttattga tggcggccgc ggttgcgatc
    27601 attttatcca cggccaatac ttttttgatg attccctcga ccaatattac acgcgacatt
    27661 tatcagcgtt ttatcaatcc gcaagccgat cagaaaacga tcattaccat gcagcgtgta
    27721 ctgatcgtag tcatcgcgct ggcggccttc ttattggcga cgcagtttca gaccatacta
    27781 gccatggcgt ttaccgccta tacgatggtc ggtgcaggca ttacgccggt actgcttgcc
    27841 agctttttat ggaaacgcgt tacgccccag ggcggtgtcg cttcgatggt tgcgagtatg
    27901 atcgtgaccg tcggtatcac ggtagccaat gccgtcatgg atcaaccgct gcttgaagct
    27961 gattatatta ttattcctgc aattatttct tcattgggtt gtttgatctt tgtcagcctc
    28021 atgaccaaac cgtcgccgga agaaaaatac gcgcccttta tgagcgccgg gcaataacac
    28081 actatgtttt ataggacagt taaaaaggca ctgctctttg tagcggtgcc tctattttgt
    28141 atcaataacg attcccttct ttacgcacaa acgacctccg atagtctcca tttatcctcg
    28201 acagatactt ctacgattcg gcccgactcg gcgatggtca aaccgtttcc ttatcgttcg
    28261 gttcaacatt tttttgaaaa aatagatacg acggaaaaca ttcaacgtgg agattcttca
    28321 ttccggtata cgattttgcc gcatgattcg ctgctgcatt attatcatga agattttgcg
    28381 gacatgctga tgcaaataac aggaacttat ttacacgatc agggtagtta tggtaaacct
    28441 atctcggtaa catggtcggg attatccaat cgtcatatat taatattgct cgacggtatg
    28501 ccgctcacag aaccggattg gggttggacc aatcttaatt atgtaagcct tgaaaccatt
    28561 gatcgtatcg aggtgtttcg gggtaatgcg tcggtgcgat atggcgcaca agccgcaacc
    28621 ggatatatca atatcgtatt gcgtcaagcg gatgcggaca agccactcac atcactcaag
    28681 tttcgttcgg tgtttgatga tttccgcgat gtgggtgtat ttttcgggcg aaattttggt
    28741 tcgcgcgtac agacgtatgt cggcggttcc agccgcggca cgccgggtga acaactggct
    28801 atcggtctca gcggaggtac cttggccaat tttaacagta cccggtactc cggaaaaaac
    28861 atttttttcg gcggtaaagt actgattcat gagaacattg cattgcagat tcatcgccaa
    28921 atctatacgg accgatacga tgcctatgga cgcgatcgtg atccgctaaa ggatgaattt
    28981 gatttttcaa cacgcggcgc agagcgcaaa gacgagcgcg acgattggca agcacgttta
    29041 gtcgtaaata ataaagcgca tatgtggacg acagaatatc aaaacacgga tatcgtgcga
    29101 cgctccacgg gatttacgta tcgtttagtt ccacgctact acggagtaaa aaatcaaacc
    29161 taccgtaccc ggtatgccgg cgatatgggg gtttggtcgc ccgtagtcgg cgcagcgtat
    29221 gatcgaacat cgtttgatac gctcaacacg cgttttgata catttcagat gacttcgtta
    29281 tttacggaaa atacatttcg atgggcatca tggactgcta tgatgggtat gcgctatgat
    29341 catcattcgg tatatgatga cggggtgacg tggacagcgt ctgtggcgta tcctatacaa
    29401 aagaaattaa cccttcgcgc tgatgtcgga agtaccgttg tttttccatc gttgcatgac
    29461 gaaatgatac atacgacatc gttgtattcg gatgcatttg ctgacggata tacccgcagt
    29521 tatttttttc ctaaggattc acttagttta cttccggtga cgcgtattcg tacgatctcc
    29581 ggcaccatgg cgcatcattc atcgggtcga ggtttgatcg tgtccgctac gatttatacc
    29641 aactttatta aggacggtac gtattataca ccggttcacg ttcataccga caccgaacgg
    29701 gtagtaccta aaaccaagac cagcggaaga acatggggat tcgatattga agcgtcccat
    29761 ccgttttctt ttacgacatg gacactgcgc caatccgtca gccagggtga tcaggtgttt
    29821 cgtagcggga caccgtggta tcgggcatcc ctgaccggct atggcgaaaa aatggtgttt
    29881 caaaacaata tgcgtctaac gggtacaata acggcgtcgt actggaataa gccgacaggg
    29941 tacacgtttc aagatgcgcc ggcgatgtat tttgtgccga tagcatctaa ccgatcagga
    30001 ggttttcttt tctttgcgcg cgtatcggcg tatgtgggcg acctgcagat tttttatgaa
    30061 gcggaaaata tttttagaaa tcgcttcgaa cttatggatg gttatgctgt aacgccgcag
    30121 caatggcgtt ttggtttggt gtggaaacta tataattgaa tatttcagag aagatagcgc
    30181 gggttaatca attttaataa ttgttttacg tttatgcttg cggctatttg gttttttaac
    30241 tttattcatt cacacattat ttgatggtaa aacacatggc ggatcgttac aaacaattta
    30301 ccgaataccg gcagaaaatg aatgaacgca ttctggcgga ggataataag gtcatcaaac
    30361 ggtttttcgg gcttgatacg ttgacgtatc aggacggagc gttgaatata aaaaccaaag
    30421 aaatgctcgg ccttgtcgcg tcgcttgtat tgcgttgcga tgactgtatc gcttaccata
    30481 tcaataaatc caaagaagcg ggcgtgactc gcgaagaaat gtttgaagtg ttcagcgtcg
    30541 gactggtggt gggcggttct atcgtgatac cgcatcttcg ccgggctgtt gaatttttag
    30601 atgaagtcga acgtatcgaa accacggagc cgcattcgca tggccattaa gaaatatctg
    30661 ttttggggta tattagcagg gattgtgatt tggttgaccg taagccgtct tttgaaggag
    30721 gcgacttctt caccgggcgg aggaaatcgc ggcggtggcg gcaatgcacc gatgataatc
    30781 aaagctaaaa tcatcaagcc gcaaactttg gctaataaaa ttcagtccac cgggtccgtc
    30841 attgccaacg aagaagtcga attacgcagt gaagtttccg ggcgtgtggt gcgggtttat
    30901 tttcgtgaag gcagtatcat caataagcat gatcttttaa tcaaaatcaa cgatgccgaa
    30961 ctgacagccc aactggagcg cgcggagtct aaacgcaaat tggccgaatc caacgaagtg
    31021 cgtcaacgcg gactttttga aaagaacttg gccagtcaaa atgattatga catagcgctc
    31081 aatgaactga atgtagccaa agcggacatt gaattgatca aagcgcaaat ttccaaaacg
    31141 gagattcgtg cgccgttcaa cggtgtgatc ggtttgaagt atgtcagcga aggcagctat
    31201 atcacgccga caacacgtat cgccagccta caaaatatgg atgcgatcaa actggatttc
    31261 tccattccag aaaaatattc cggcgccgta cgtgtaggcg atgatattga gtaccggatt
    31321 gaaggttcgg ataaattata taaaggcaaa gtgtttgccg tcgaaccgaa aatcgagttg
    31381 gccacacgca cgctgcaact gcgtgctatt tcgacacaga ttgaccagcg cgttttgccg
    31441 ggcgcttttg caaatatcac tttgatcctg cgcgatattc ccggcgcgct gatgataccg
    31501 accgaagcgc tcatgcctga actgaaaagc caacgtgtgt atgtctataa atccggaacc
    31561 gccgtttctc aaaaagttga aaccgggatt cgtaccgatg tggatgttca gattacaagc
    31621 ggtatcgctc cgaatgacac aatcatcatc acaggtctta tgcagttgcg caatggtctc
    31681 ccggttcgca tcagcgatct gattcagtaa ctatttacag ctaaaatgcc cgtatcgcat
    31741 caatttaaat tttgaagcga tcggcgatca ttctattgag aaagcttgtc tatgagtctt
    31801 tcagcgattt gtattcgtcg tccggtactt gcgatagtga tgaatatcgt catcgtgctt
    31861 tttggcgcga tcgctttcag ttatcttggg gttcgcgaat ttccctccat tgatccgccg
    31921 atcatcaatg ttcgtacgtc ctataccggt gccaatgcgg aagtcatcga aacgcaaatc
    31981 accgaacctt tggaaaaagc catcaatggt atagcgggta tccgtacgat cacgtcgtcc
    32041 agcaatcaag gcaccagcaa tattacggtc gaatttacac tggatgtgaa tcttgagacg
    32101 gcagctaatg atgttcgcga taaggtttcg caagccgtgc gtcaacttcc gcaagatatt
    32161 gatgcgccgc cggtagtaac caaagccgac gccaattcgg atgccatcat ttcgatgacg
    32221 gtagagagca cgaccaaaaa tcaattgcag ctcagtgatt ttgcggaaaa cgtcattgcc
    32281 gagcgacttc agacgattcc cggcgtgagt agtgtacaga tctgggggca aaaacgttat
    32341 gcgatgcgta tctggatgga cccggctaaa ctttcggctt tcggattgac ccctttggat
    32401 atacgcaatg cattggatcg ggaaaacatc gaagcgccga gcggaaaaat ttccggcgat
    32461 gcaacagagc tcgtcgtacg aaccatcggt aaattaatca ccgaagatga ttttaataat
    32521 ctcatcattt cttcgacggg tgaacgcacc gtgcgtctta aggatcttgg ttacgcgcgg
    32581 cttgggcctg aaaatgaaga aacgattttg cgccaatcgg gcgtgccgat ggtcggatgt
    32641 gcgatcattc cccagccggg ttcgaattac atagagattg cggacgagtt ttacaaacga
    32701 tttgagcaac tcaaaaaaga tctgcccaaa gatttttcgc tcaatatcgc tctggataat
    32761 acgcgttttg tcaaacgttc cgttgcggaa gtccaagaaa cgctattgat cgcgattgcg
    32821 cttgtcgtac tcatcgtgtt tttgtttttc cgcgattgga tcgtagcggt tcgccctttg
    32881 attgatattc cggtttcact catcggggcg ttttttatta tgtatacgat ggacttctcc
    32941 atcaatatcc ttacgctgct ggccgtcgta ctggccacgg ggcttgtggt ggatgatggt
    33001 atcgtcgtaa cggaaaatat ttataaaaaa atagaagaag gcctttcgcc gatggaagcg
    33061 gcgtttaaag gttcagccga aattttcttc gctgttattt ctacgtccat cacgcttgcc
    33121 gcggtttttt tgccgatcat ttttttagaa ggttttgtcg gacgtctttt tcgcgaattt
    33181 ggtattgtga tcgcgggtgc cgtattgatt tctgcatttg tatcgctgac gctcacgccg
    33241 atgttgagtt cgcgcctttt tcgtcgccag caaaagcata cgcgttttta tgaattgacg
    33301 gagccttttt ttgcagggat gatacaggcg tatggtaatt cgttgcatgc ttttatgaaa
    33361 cgtcgctggt tagcgttggc tgttctggtc gtgtcggtgc ttttaatttt cgtcgtggga
    33421 aaacaattgc aatccgaact ttcgccgctc gaagatcgca gtttcatacg ttttcaggtg
    33481 actgcaccgg aaggttcatc ctttgattat accgataatt ttatgttcaa tatggtgcag
    33541 ttggtcaatg attctgtacc ggaaaaacgc atctttttga ccgtgacggc gccgggattt
    33601 gcgggtacgg gcgccgttaa ttcagcattc gggcgattgg tgttgattga acctgacgaa
    33661 cgcaaacgat cacaacagga gattgcggct aaactcacca actatacacg caagtattcg
    33721 gaagcgcgta cgtttatcgt gcaggaacaa acgatatctg cctcgggcgg cggtgcgcgc
    33781 ggcggtctgc cggtgcaata tgtactgcaa gcgccgaatt tcgagcgcct caaagaagtt
    33841 ttgccgaaat ttatggaaga agtggctaaa gaaccgacgt ttcagggttt tgatgccaat
    33901 cttaaattta atcgccctga attgcagatc acgattgatc gcgatcgtgc ccgtagcctc
    33961 ggtgtttcgg tgttagatgt aggacagacg cttcgtttgg cgctgagtgg ccagcggttt
    34021 ggttattttg taatgagcgg aaaacaatat cagatcatcg ggcaagtcaa tcgccgggat
    34081 cgcgacgaac cgctggattt gaaatcattg tacgtccgca accgcacggg cgaactgata
    34141 cagatggaca atctggtcaa agtcagcgag caaagcggtc cgccgcaatt gtatcattac
    34201 aatcgctatt cctccgcgac cgtatcggcg ggacttgcgc cgggaaagac tatcggtgac
    34261 ggcatcgtag cgatggatcg catcgccaaa aaggtgcttg atgattcgtt ttctactgca
    34321 ttggcgggtg cttcgcggga tttttcagaa agctcttcca atattttatt tgcattcatt
    34381 ttggccctgg tactcattta ccttgtgctg gccgcgcaat ttgaaagttt tgtggatcct
    34441 tttattatca tgctgacggt gccaatggca cttgccggcg cgattttgtc gctgtggtgg
    34501 tttcagcaaa cactgaatat atttagtcag atcggcatta tcatgctgat cggtctcgtg
    34561 acaaaaaacg gaattctgat cgtcgaattt gccaatcagc ttcgtgaaaa agggaaaaac
    34621 aaaatggatt cgattttaga ggcgtccgtg ctgcgtttca gaccgatttt gatgaccagc
    34681 cttgctacgg tgcttggtgc cgttccgatc gctttagcgc taggcgcggg ttcgattagc
    34741 cgaattgcca tgggtattgt gattataggc ggtgtgattt tttcgctgat tctgactctg
    34801 tatgtgattc ctgcgattta ttcttatatg aagcatgagc acaagcccgg tcaggaaaca
    34861 aatatcgctc cattgcaaca aacagaatcg ttatcaaagg agcatgtata gaaaagtgtt
    34921 taggtgtaat agataaaaac aaaacccggg gcattaaatc tcccgggttt ttttaattct
    34981 acaacatcaa cggaataccg ttttttttaa aatcgtttcc atcaaacacc atccggtaaa
    35041 tgatgattgg atgagattga tacctacgaa cgccgtaaag aacagccagt tgggatgaac
    35101 ccaataacct aaggccagac taatcagtac gaaagtaccg gcgataacac gaatggtttt
    35161 ttctaacata gtatctcctt atgcaaattg ttcgacatgc atttgaaagg cgcgcatcgg
    35221 atgatcgaga tccatcgttt gtccatgatt gcggatggct tgcatcagtt cagcagcctt
    35281 tgattcatca acaaaagcaa aaaccagatg cgaataaatc ggcgaagccg tgtgtgcaaa
    35341 ccaattggaa gaatcgcgct ccgtagcatc actccggtag ccacgaattt ccgactctga
    35401 aaaaatgatg acgtgatgtt cgcgaaaaag tttacgcaga tcctgcgcgt actgttcgat
    35461 cgtcatgact gcgatcagtt tcatatcaat tctccttcga ttcgttttcc gttgtttcaa
    35521 gaggcacact cgcttgcggc acagcctcgg attcgtactg cgctttttcg gtcatataat
    35581 aaaaaagcgg caccatgatc agtgtgaggg ctgtcgaagc gatcgcacca ccgataagcg
    35641 aaatagctaa cccttggaaa atcggatcaa ataaaatcac aaatgcgccg atgacaaccg
    35701 cgcctgaggt cagaagaatc ggcgtagtac ggacggcgcc ggattcgata atggccgttt
    35761 tcagcggcac accgtcctgc aagcgcaatt cgatgaaatc aatcagtaat acggaattgc
    35821 gaaccatcac gccggccaat gcgatcatac cgatcatcga agtggctgta aaaaaggcat
    35881 ccatcatcca gtggccgacc agaataccta ccaatgacag cggaatcgca accatcatta
    35941 caaaaggtac tttgaaggat tgaaaccaac cgatgatcaa aaaatagata atcacgatca
    36001 cggcggcgaa ggcggcaccc agatcacgga acacttcgta agtgatctgc cattcgccgt
    36061 cccacttcat cgaaaaattt tcttcaagga aaggttgttt ggcgtatagc gtttgcagag
    36121 tgtacccttc aggtaaccgg atttttttca tcgcttcgtc gatgtttaaa atcgcatata
    36181 cagggctttc cacttcacct gctacatcgc ccgttacata aaccacgcgt tgctgatttt
    36241 tacggtagat acttttttca cgcgtttgag gatagattct gaccaagtcc gataccgcta
    36301 caggagtgcc tgtcatcgcg atgacatgga tgtttttgag gtcatctacg gacgaacgct
    36361 gagattcggc gagttggagt tgaatgccta cggtttccag ttctttggcc tgatacagtg
    36421 cgccgatttg tttcccgttt aatgcaattc ctaaaacgtg ataaatctgc tgcgggctga
    36481 caccgctttg tgccgctttt tctttgtcaa tttcaaatcg gtattcggtt tgatcgtctt
    36541 cgacaagcca atccacatcc actacgccgg gcgacgtttc aaacgccgta cgcacctgat
    36601 gggctacctc aatttgttta gtataatccg gcccatatac ttcagcaacc agcgtggaca
    36661 ataccggcgg gccgggcgga acctctgcaa ttttgatgcg tgcgccatag gaagcgccga
    36721 tctttacgat agccggtcga atacgtttgg caatgtcatg gctttgttcg ctccgctcgc
    36781 ctttgggaag aaaattgact tggatatcgg cgacattgga gccttgtctc agatcataat
    36841 ggcgtacaag cccgttgaaa ttgatcggcg cggaagtacc ggtgtacatc tgataatggg
    36901 ttacttccgg cacgttacgc agataggcgg caatctcttt gctcaacgca gccgtttttt
    36961 ctagcgtggc gccttccggc atgtcaatta caacttgtaa ttcattttta ttatcaaagg
    37021 gtaacatttt tacggttacc aatttgaagt acatcagaga catggaagcc agcaatagca
    37081 cgacgacact tccgatgaac caccagcgtt tttttgcgtt ttcgagtaac ggaacaaaaa
    37141 tacgggtata tagtttataa attcccgtcg tttcaaggcg atattctttc tccgaatgcc
    37201 cgtgcgtatc gccgcgcaaa agacggtagg cgaggtacgg cgtgatgatc agcgcgacga
    37261 acagtgaaaa cagcatcgcc agcgaggcgc cgatcggcat agggctcata tacgggccca
    37321 tcaatcccga cacaaaagcc atcggcagta cggaagcgat caccgtaaac gtcgctaaaa
    37381 tggtgggatt gccgacctcg tctatcgacg caatagccgc ctgtaaaaac ggcaatttcc
    37441 gcattttgaa gtggcgatgc atgttttcaa ctacgatgat ggcgtcgtcc acaaccaatc
    37501 ctgtcacgaa caccaacgca aaaagtgtaa tacgattgag cgtatagcca aacatgtagt
    37561 acacaaaaag tgtcatagca aaggtcaccg gtacggtcat aaaaatgacc aatccgctac
    37621 gccaccccat ggtcaacgca acaagtaccg tcacggccaa aatagctgca gaaagatgtt
    37681 tgagcagttc gtgtactttt tccgacgccg tttcgccgta attgcgcgtg acggtcatgg
    37741 ttacgtcgga gggaattaat ttgcccttca atgcttctga cttttccatc aggcgttcgc
    37801 cgaggcccat cgcatcggag cctttacgtt tggataccga caacgtgacc gccggatatt
    37861 ccaacgcttt atgttcaccg gcgtgagcgg ccccgtaacc gaacgtcaca taatctttga
    37921 tttcatccgg accgtcttcg atctgagcga tatttttgag atagacaggt ttgcccaggt
    37981 gcatgccgac gaccaattgg cgtacgtcat ctgcggtttc tagaaatttt cccgtttgta
    38041 ctaaatattc cgtgttgcct tgattaaatt ttcctgccgg gccttgttgg tgggcggatt
    38101 ggaaaaaagg gataatcgtg agtggatcaa ggcgatacgc cgccattttt tctttgtcca
    38161 atatgacccg cacttgccgt gagcgtccgc cgataatttt cacttcggac acgtcgttga
    38221 tttttttgta ttcagaactg agttctgcag caaaacgtcg taaatcaagc gcatcatacc
    38281 gggcgctcca tagagtcatt gcaaaaatcg gtacgtcgtc aaccgatttg gatttgatga
    38341 acggcggcct gacaccggcc ggcatcttat ccatgttttt gaaaacttcg tcgtagagcc
    38401 gaatgatcgc gcgctcggca tcctcaccga cgacaaaacg tacgatgagc atgccttggt
    38461 cctgcatgga cgtcgagtac acgtattcga cgccgcggat attggaaatt attttttcca
    38521 gcggctggat gactttggtt tctatttctt taggttcagc gcccggatac tgcacaaaaa
    38581 catccgccat cggcaccacg atttgcggtt cttcttcgct cggtgtgaga taggtggcaa
    38641 aaagaccgat tgctaaaaac cctgcgatga gcaacggcgt cagtttagaa ttgataaacg
    38701 cttgtgcgat gcgtccagaa aatccggttt tcatgggcga acctccacac gatcaccgtc
    38761 gctcaggcgt tccgttgatt cgtatataac acgatcaccg aaatcaagtc cgctcaatac
    38821 ttctacttca ttgccatagc ttttacccgg gcgaatccat cgtaacaaag cctggttggc
    38881 tgcgctgact acgaaaacac cttccagttg gccgcgccga tgtatcactg aatccggaac
    38941 agtgatcata tttttttgac cggcgcccgc atagatgcgt acgaacatgc cggagcgcaa
    39001 cgttttcttc aggttttccg ttattccatg aagcttcact tccgcgtcat attggcgtga
    39061 gccgggaact gcagaaggac tgagggcgct gacatagccg tttaaccgat gcttcggatc
    39121 tgaagaaaac tcggcttcga cggcatcctg gatacgaatc gaagcggcgt cgctttccgg
    39181 gatcgggata atgactttga aggatgaaag atcctcaata accatcagag gcatacccgg
    39241 tgcagcggta ttgcccactt cagtgagttt ttggacgacg taaccgtcga tgggcgctgt
    39301 aatatccgaa tacccaaaca cgtcgttgat ttctttttcc atttcttcga cggctttaag
    39361 tttggcctgt gtcatcgcgt acgccatttc cgtgtcgtcc atttcctttt gtgtagcact
    39421 tttgcgggcg tataattctt taatgcggtg gtaattggat tccatatttt ttaaagcggc
    39481 ttccgcttcg attttattgg cggcgatttg cgcgcgtttg gcgctcaagt catcgctttt
    39541 gattttgatc agcagttggc ctttagcgac gcgatccccg atgtgggcat gtattgccag
    39601 gacttgaccc atgattttgg tgctgagcgt aatgcgttta tcgccttgca atgtaccggc
    39661 caaggcggtt ttttcgttaa ccggtttttg acggatcgtg attgtgcgca ccggtgttgc
    39721 ggtttgattg ggttgatttt tctccggaga accgcatgcg accataacga tcatcatgac
    39781 ggacaaactt aacccgataa aatatttctg catttttttc ctcgtgtata ttaatttttt
    39841 atggattgtt cggtgagtaa ttcgaccgta aacatcgctg catgccaggc aaaccgggca
    39901 tgtatcaatt ggaggcgtgc ttgcgataaa gccgcttcgg ccatcagcaa atccgttgta
    39961 cgatctaaac cctgatgata gcggtcggaa agaacacgat aattttcttc ggattgctgc
    40021 acggcctgtt cagcgataac gacttggcgg gttgaaactt tccactgacg aagtgccgcg
    40081 ttaaggtcat attgactttg cgtcagcgct tttttataac cggtttcggt ttgacggtat
    40141 tgcgcgtttg atttctgaat agcggaaatt tggtcgaaac cggagaaaat atcccagcgc
    40201 agcattgcgc cgaccatcca gtttttagct tgccggccga acagggatgc gtcattgtat
    40261 tcgtaagtgg caaatccatt gatactggga agaaatttac tccaggccat actttgcatg
    40321 gcacgctgtg cgctcatctg ataacgcatg gctcgtatat cgctacggtt tgtcatatcg
    40381 tgtggcgttg tcatttctaa ggctaatggc ccgcccagtg tgtcggtagg aataatcact
    40441 tcatcgcctg acaaacccat cagaagtttg agctgattat tcgcattatc tatcatgttt
    40501 tctgtttcaa gttgtttagc ttccagttct tttaaacgga gctcggcaaa aagtacatcg
    40561 gagcgacgga tcagtccttg atcataaaaa ttgcgcgcct gctcgctacc ggtctgggcg
    40621 gcagtaagcg ctttgcgaat aacttcacgg ctttgcaaag cgactaaaag ttggtaataa
    40681 gctattttgg cttgataggt gatatagtaa gatgtgcgtt tgcttttttc ttccatcgct
    40741 ttgacgccgg aggatgccgc acgaaaaccg aaaaaaccgt ccatattcag cagtggttgc
    40801 cgccattcca gtttggtggt ataattatct acagctttag gttgattgag tgtgaccgga
    40861 ttgaaatccg cctgagttac aatttcttgt ttaagtttta aaccgaaaac atttaacgga
    40921 tcattggtgc gggtataaat ttccgaaacc gataattgag gaagaaaaag agacaacgat
    40981 tttcggtaat cggctctcat cgcatccatt tccgcttgta cagaagctaa atcataattt
    41041 tggcgcgaag cggtttgtaa tacggagtcg agagtgtatc gggtgccgtc ctgcccggcc
    41101 ttgacttgtc cgaccagcac aaacaaaaga aaaactccaa aacataaact tttcatacta
    41161 ctccttaaac ttaactatat aaccatatag taatataata aacttttaac tccaagtcaa
    41221 gtattttatt ccgccgtttt ttagatcgtt aattcactat ttacattagc cgattacaga
    41281 aatacattta taaaacatcc ttaaaggtac gctcatgtat tatttgttga tcgcactggt
    41341 tttgttaaca aaagctgttt tggccgggga tgcgccggat tgggttattc aacccggaaa
    41401 atcagccgca tttcctgcat ctgcttatat agtaggatac ggctccgctt ctgcccaaaa
    41461 cccggacgca caaaaaattg cggaagataa tgcacgagcg gccttatccg gttcgatcgt
    41521 aaccgatctg cgcagtacgg taatttcgag gcttgaagag caagggaaaa attcggcgga
    41581 atatttcagt tcggttaccc agtcttctac cggattacaa ttgttgggca tttcgataga
    41641 aacatataaa gataaaaaac tggttcatgt gttggcgtat gccaaacgaa cagaacttag
    41701 cgccacgtac aacaaaaaac gtgaggcgtt aatttcgaat atcgctgcga tcattacaca
    41761 agccaaggcc gcagaagaat caaaaaaaat agaagatgct gccgcacttt atttgagttt
    41821 gtttccgatg tatgaagagt tgcgcgaagc ggagatcgta ttgacggtgg ttcatcgcga
    41881 aagccaagtt tctcaaccgg ataagactat cccgtcccgt acagagatag ctcaaaccat
    41941 agatcgcctt ctggcgcaaa gcattacatc cgtcgaagat gcggcacgag ccgttgctta
    42001 tcaggtatcc aaacaaatat ccggcacgac cggatcgttt ttggtgtcac cgatgacgta
    42061 tcaagattcg aaaatgagta gtcccttcgc acgttatttt caacaagcac tggagacgca
    42121 atttcaaaaa tatgcggtat gggaagttgc acggcaggca cgcggatttc aacccaaatc
    42181 atctcagatt acccgtgatc ttgtcattgc atccggcgcg ggatgtattt ttgagggcaa
    42241 ttattgggag caaggcgatc gcatcaaact aattggtcgt ttgcgcgatg ttcaaaacgg
    42301 gcgcatcatg gccgccgtag aagtaacgct ggatattgcg catattcaga aaaccggtct
    42361 ttcgatcaaa ccggaaaata tgcttaaggc cttagctgac caaaaggctt ttgctgaaga
    42421 agaagtgatc agttcggata tccagttgga tgtgtggacc aacaaaggca cggaaaacct
    42481 tctttttgct gaaggtgaaa tcatgaagat ctacgtgcga accaatcgcg ctgcgcacat
    42541 acgcattttg tataattttg ctgacggttc acgaacactt ttatatgatg atcattatat
    42601 agatgaagct aaggccaatc aggtcgttga aatacccggt gaatttgaat gtacgggacc
    42661 ttttggtgct gaaatgatgg tcgtcgttgc cagcgtagga aaactaccgg aagtgaagac
    42721 gtacagtgtg gatggtatcg cgtatctgga tgaaaaagat gccaaaaaag cggcatatgg
    42781 tacacgcggc ttcaaaaaga aagatccggc atcttccact agtcctcaac aaaccgaaaa
    42841 aaaactgacc atcactacta tcgcaaaata atctagttta gcagcacttt cgatttcatc
    42901 ctaaactatc gaacattctt ttttcggttt ttgatgtagt ccacgaatat gtattgcccg
    42961 agttcgttga ggcctgcata aaacgcacgg cctttgttgg ttttggtgag tgtatctaca
    43021 aagcgtgtca ggtaaggatc atcggcgatc ataaatgtcg aaatcacgat attatctttt
    43081 ttgcattgtg ccgcttcgtt gagcgtttga ttgacgattt tgcgatccaa accaaacgaa
    43141 tttttataca gtttaccttc ttcgcgaata catgacggtt tcccgtcggt aatcataaaa
    43201 acctgcttgt tttgattgcg tcgctttcgc aggatttgcc ttgccaactg gagcgcttct
    43261 ttggtattgg tgtgataagg gccgacatga atatagggaa tgtcttcgag atcaatctgt
    43321 gctgcttcat cgccaaacgt aatcacatct agactgtctt tgggataacg cgtgagtata
    43381 agttccgtca acgccaaagc cacttttttg gccggagtga tgcggtcttc accgtataaa
    43441 atcatgctgt gactgatatc aatggccaat accgtagcgc aggtagattt gtgttcggtt
    43501 tcatacactt caaaatcatc ttcacgaagt tcgatgtcct ctccgcgttt tcgtttcatg
    43561 gcattgttca tcgttgccat aaaatcaata ttggacgggg gatcgccgta ctgataaggt
    43621 ttggtttcgc tcaagcgttc aacgccttcg cccgtgtgat tcgtttcatg catgccgccg
    43681 ggcgattttt tgagagatga aaaaatctcg tccagagaat cctgtcggat tttccgttca
    43741 cctttaggcg aaaggacggg agcgctgcca tcttgggatt cttcgatata cccttcctgg
    43801 acaagacgat cataaaattt ctgaaaatcc atatcttcgt taaagatatg atactgacga
    43861 tcgatctgcg tcatatagct cagagctttg cttacgtcac cgtccgttag cagtaagagc
    43921 tgattaaaga ttttcatcag atcgtcgaac ttcattttgt gattcgacgg tcccggcata
    43981 aattgtgaat attcaaaccg catgagaggg acctaatttt ccaaattttt tatgcagtac
    44041 aaaacacaaa accgtaaaaa gaatcagacc gatgaaatac agatgcacaa caaccgaaat
    44101 actgaccgtg aaagcgatca taaggtaggt taggcgtttt ccggttttta gaatggatgc
    44161 ttcttcgaac ggattacaaa cataatgaat gccgaaatga acgagtgaaa gcaggctaaa
    44221 taaataaaac aacggatgaa ttatccaacc ggcagcagaa gataacaaga aagacagcgt
    44281 caataatcca agcacataga acttgctttg ttttaagccg agaaagacta tggttgtaca
    44341 ttttacaggc gtcgcatgac gatcgctttc gtagtcgaga taatggaaaa ccatcataat
    44401 cgcgatataa atcaatccgg ttattacggc cattagaata atagcgctat ccaattggcc
    44461 ggtctgaacg aaataggcgc cggtcatatt gagaaaaatt cctgtaaaac cgccgaacca
    44521 ttctccggca aaaggtctgt aagcaaactg taacggaggc aatgtgtaaa ataaacacac
    44581 gaaatatccg attccgaaga accaaaaaat ttcaggtgaa gtgcgttgcg acagcccgaa
    44641 catcaaacca agcgctacaa tcgtggtcac atcgaaaata tgccataatt gggtaatcgt
    44701 ggccaagccg gatttgagca ctttactgcc gccggatttt tcaccgagct tgcggtattg
    44761 gtcggcgccg cttttccaat cggtgatttc gttgacaata tgtgccggaa atccttggac
    44821 aacagaagcg atagcggctg taagtaaaaa attgacccaa tcaatctctc ctgtctgata
    44881 cactgccagc gatacgctga gtacgatggt caatacggcc cataccaata ccgccagaat
    44941 tcgatacacc ataaaccagg ctttaaacgg agggaaaaaa gaatttatat cgttattttg
    45001 catggttatg atttggtttc cggaggttga gtcaatataa agttgaccgt cgccgtggcg
    45061 acaagtgcat cggattcgtc tgttatttta acttcacaaa cattgattcg cgcgccgcga
    45121 cggatgacga cggcatgggc aaagatagta cttttagcgc gtgaaaggta ggtaatatgc
    45181 aagtccaccg tagcaaaact catctttccc tcaaacgcgg tggacaatgc aaaagccgcc
    45241 gccgtatcgg ccaatgcagc gacgatgccg ccgtggatcg taccatgact tcgcgggcca
    45301 ttggtgatag cctcacggtg cgtaagccgc aggcaggcat acaactcgcg tagttcttcc
    45361 aaagaaaatc caagcaaact gttagccgga tggtttgcta aacgagtagt cagtaatgtg
    45421 aaaagcgcat cggttaccgg cgcttgatgg gtatatttgt ctttgtggtt tttcatgttg
    45481 agtgtttaca gcgcatataa gaataacata aaaaactatg gtaatttggt ttagaaatca
    45541 aattttcgcc tacagattca caaaacgtat ttatgcgtac ccacacaatg atgtaggttg
    45601 tttcgccgag aggtttgatc catattaata caatgacaaa ccaactgcga ttaaattcat
    45661 ccgccgggcc gtcggcatta ttcatcaatt cgccttgggc tcttattcac agggacgaag
    45721 aggctaatgc cgccatgtta gaatggaaaa atcttgtaac cggtgaaacg tataaaca
//
LOCUS       DAOQGF010000010        45452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_332_length_45452_cov_6.301672, whole genome shotgun sequence.
ACCESSION   DAOQGF010000010 DAOQGF010000000
VERSION     DAOQGF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 45452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45452
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_45452_cov_6.301672"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..506)
                     /locus_tag="PK937_03005"
     CDS             complement(60..506)
                     /locus_tag="PK937_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH31569.1"
                     /translation="MLSLLMILLSAATFGYVLYPLFSKNVMPYGLKPIKDQHRDDLAN
                     MKQKLYTDIKDLDFEYGIGKIAEADYQNLRQATLKELADVLKRMDAIEQGDNGNGKIS
                     DEYLEHLIQSRRAKTIVVQKAICPSCKHENMKTAKFCSECGAKLNA"
     gene            complement(510..1034)
                     /locus_tag="PK937_03010"
     CDS             complement(510..1034)
                     /locus_tag="PK937_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNH31570.1"
                     /translation="MKKLKIYFIASILITSALYSQGGSAHTTKLNAEQNEMFNKISGE
                     LICQCGCNLVLGTCGHINCPSAVPMRNTIEDMIVAGKSHDAIIQYFMTEYSFRDNPPA
                     GKAILSQPETKGFDLLAWIMPFLLFVIFIIVVVFLIKKITAGKTAPVTVSPAHPADDP
                     YTQRIEEELKKMDR"
     gene            complement(1021..3036)
                     /locus_tag="PK937_03015"
     CDS             complement(1021..3036)
                     /locus_tag="PK937_03015"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNH31571.1"
                     /translation="MGQIGNYALIVALAMAAFAILSAFLGVHYKRGDLVRSAQNAVYV
                     VFAMVVVAAGSLIYLMVTGDMTYEYVASYTNRDLPLFYKYTFWAGQAGSLLLWSLLLA
                     AYSFAVVYRYRNSFGELMPWVIVVTMATQVFFLALNLFSSNPFDVLVTHHGDGSETLF
                     RPQDGRGLNPLLQHPAMVIHPPTLFVGYVGFAIPFAFAMAALISGQLNDEWIKLSRRW
                     TLFAWLFQSAGIMMGGWWAYEELGWGGYWAWDPVENASLMPWLTGTAFLHSVMIQEKK
                     NMFKVWNVSLIIATYLLCIFGTFLTRSGVVSSVHAFAQGSIGIYFISFITLSLLVSVW
                     FVIKRLPMLKSEGQLDSALSRESSFLFNNLVLLISCFAVLWGTVFPIISEAVTGEKIV
                     VGAPFFNKVNIPIALFLLFLTGAGPLFAWRKTSAASLRKAFLLPTIIALAGGVVIFMF
                     VTQHFYGLMSFVLNIFVAAVIAIEFAKGTSARMRSNGENVLIALKNLTLKNKRRYGGY
                     IVHFGIVVIFVGITGSIFNYETKTELGQGESRQVKDYLLKIDKVDFTNTPNYQSSYAV
                     VSVYKDDKKIDELKPEYRMYRSSQQPTTEVALHKTLKEDLYLVFTGLDDTEKKAVLQI
                     YVNPLVSLVWLGTEILVFGTLICLLPAYQEDRKSVKSMRKEKTLEKA"
     gene            complement(3083..4813)
                     /locus_tag="PK937_03020"
     CDS             complement(3083..4813)
                     /locus_tag="PK937_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH31572.1"
                     /translation="MIIKFRYKLIFLVTVTLTLAAVAYVIVSLGLAKNIETDITDRLI
                     KSNGTYEEFNNSYFEQLILQSRSVSDNPKFAAQLSTGDQKTVQQALEETNQIGEFKSD
                     IFVALSPSGQVLAFVGTELSASMNLSQRPEVAAALEGYDNGGFWEENGKLLRVAASPT
                     IVNNQVLGGIVLGYDVTDTVATKIAKVTTSSIAFIINGKIVATDKFDNRNELLAQFKS
                     SQSFLDQAMIRKTSSDPFQFTIHGERYLAVAHPVFAATGSQGQERPVIATYVFFSSLD
                     KALEPLVASQRTMVFVGGGVTILAFMIALVIISGVTKPVHQLSDAMHKVQSGDLNIML
                     QIKSRDEFGELGKNFNEMTQGLKQKERVENLFGKYLSPDVAKKVLSEQSVDGILKGEK
                     NRLSVMFTDIRGFTPMSRGMDPQELINLLNSHFDEMIDIIDSYGGTLDKFIGDAIMAF
                     YGAPVHYDDFYMRAINAAVQMQRAAEKFNFQRKLEGHPPINIGIGINTGEVVVGNIGS
                     NKRLEYTVIGETVNIANRLCSVAKAGQIIVSQSTYDLLPNKSIASPIERVTLKGVAEP
                     VTVYEILWKS"
     gene            4990..5802
                     /locus_tag="PK937_03025"
     CDS             4990..5802
                     /locus_tag="PK937_03025"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNH31573.1"
                     /translation="MTLIIDAHGQNLQFDKPPERVISLIPSITESLFELGLGDRIVGI
                     SKYCIYPEDGVRNKPKVGGQKNPDFDKITQLNPDLIILNEEENKPEHIALFKKNYRTF
                     VTFPRIFVQAGKLLDQLGLIFHISDRTKVFSEQIRLDHSRLTVLSPTRLRALYLIRRN
                     PWMSVNRDTFIHDILSLVGLDNVFANQSPRYFKVTREMILNAQPDVIILPDEPYRFLK
                     KHIADFDGLDIPAVKNCNIFLTDGTYYCWYGTRTARAIKYIDQNIIHRIVTC"
     gene            5796..8828
                     /locus_tag="PK937_03030"
     CDS             5796..8828
                     /locus_tag="PK937_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNH31574.1"
                     /translation="MLKLLFVFVTLLSLSLQAQYYDNYFGTNKVQYTHFEWNILETEH
                     FEIYYYPEMRELAERAGGLAEEQYAELQSRFNHALIKKVPMIIYSSHMHFQQTNTISA
                     ELPEGIGGFFEFFKGRVVLPSDGSTTHFRRVLRHELVHVFMNSKVNRIISQHRKFNHP
                     GPPLWFTEGLAEYWSGQPDFQAEMVIRDATLNGTLLPVQEFWRIDGSYYMYKLGENFL
                     HFLTENYGEEKVLQLMENIWKFDEFQKVVTHTVGKNYETLSQEWLYWLKKKYYPTLSQ
                     SDMPFAISQTITKVGFSSKPAYLFDGTNEEIFFVANRDGYTNIYRQNLKEAKAKVFIE
                     GERTHEFEAFHFFKSKIHVNEQGKIAFATKSGERDVLYVYNTQTGRKEHQYTYKDLTG
                     IISPHWAPDGKSIVFSGLHMSGNSDLYTVHVSTDETDGILTRITNDFYDDRDPAWSPD
                     GKTIVFSSDRAGFSPEGTYNLFIYDLETARTFQLTSGKYQDASPAWSPKGDYIVFTSN
                     RQGSPTNISVVPFIYQDQSFIETSTRGALQDSIYTHYAPRQLTDLTSAVFDPIWTKDG
                     DLVMTAFEEFSFKLRRMEKAYDKARQLVPTERKKIEGVRYPWTTPKVSIIGSNTRSYE
                     KKYSLDFVQGAFQIAPTYGYGGGATIGYTDMLGDDQYYLTVFNTGQTTSDILTDWNFI
                     LTKVNLGQRVKYAYGGYRFKGEFTQIGDQGLPETVHEERYGGFFQVAYPLSKFERIET
                     GISVSRYQRGNAFGALLPLDGVSIGNNVGYVYDNSLWGITGPIDGRRAMLSLGYTSDA
                     YKSQQNFYTIIADYRQYFRITTRSAYAFRTMTQWNDGKNPSNFVVGGSWDLRGFPRWN
                     MPGTRFFVMNHELRFPLMDAIQLRFPFVGLGFRSIRGAIFTDIGNAWGVQKTDGRVIL
                     GHQKFDGFLGSVGTGFRINFLGVMVLRFDFGKMFDARGYKFGKIRLPGNRDRFVETKL
                     NTNKRSEGFGGIDIDGNIIEKRRWSRGIFFQFWFGADF"
     gene            8828..9979
                     /locus_tag="PK937_03035"
     CDS             8828..9979
                     /locus_tag="PK937_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNH31575.1"
                     /translation="MKTILVCIILIFQLMACGTTGNIRIAAYFKKSTTSPWSNIGGNP
                     GRTGYAINELTLPMQKVWTKKLSTSLGKAIVVSDSIIYTTTHDGRFYAIHSGSSQTVG
                     YIKFTHASQAGVAISGQKTAIGISNGKQNFITHQVFDGKFLTIKNTGAIETNPLIYDD
                     LAYAGTLSNTAVCVDMNNGETVWSYTTSAPVHASPTISGSAVYFGDDDGILFAFNRFN
                     GKLLWKLKLEGPILSAPVADDSLVYVASNDSTLYAIHQKEAVIAWHYRIGYDKPGNFY
                     ASPAVDEKLLYIGATDGRLYAFEKRSGNLVWMYETKGPISIAPVMSKSIVFIGSQDKS
                     IYALNKYTGVLEWQFETEGRIKSEMAIYGGSLFVGSENNMLYKFSGKLR"
     gene            9976..10800
                     /locus_tag="PK937_03040"
     CDS             9976..10800
                     /locus_tag="PK937_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNH31576.1"
                     /translation="MNRHLQSLLYLFTLGILVPAMTYCQNKTPYGYLKIISHENPAFI
                     YLDNIAYEWENGKVFKLLVGRHTLAARRDNGLQTEQLDFLQNITINRDDTLSVYLRFE
                     KAITIQTKTPNSASVFINDSLYGKTPYLFIPETGNKYNVRISKTGHWDTTFTIVSSDT
                     PLMMGLRPKNHEMKSLNDNEFVNERWRKQGIHSYKKHLLIAGLSLIVTGYYTAANKKT
                     ADNAFEKAKLARRSGNIALSKKLTDKTRRYDDRARIGFIGLQISSAGFATLLLLSR"
     gene            10892..12118
                     /locus_tag="PK937_03045"
     CDS             10892..12118
                     /locus_tag="PK937_03045"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNH31577.1"
                     /translation="MKKFNIPLFYRSSIITAIKNYRRTLDQRKKDFSPTRLDFGPVEF
                     LIARHFGFCYGVENAIEISYKAIEENPGKRIFLLSEMIHNPEVNSDLQSRGVRFIMHT
                     HGEQIIPWADINRNDVVLVPAFGTTIEIENKLKDIGIQSYTYDTTCPFVEKVWNKAAQ
                     IGKESYTVVVHGKPSHEETRATFSHSRQGAPTVVIRDMKEAVLLGRFIIGELPPEAFY
                     QSFENQYSEGFDPGKDLKRIGVVNQTTMLASDTQAIADYLRNLMIDKYDLDQASVHLH
                     FAETGDTLCYATNDNQTSTYGLLSEKADLAVVAGGYNSSNTSHIVELCEEKLPTFFIS
                     SAEKIIDSQTIMHWDFHHKIEKTSKNFIPQKEKVTILLTSGASCPDTIVEGILLRLLT
                     FFSNTRDFDDVMRRFQ"
     gene            12184..15297
                     /locus_tag="PK937_03050"
     CDS             12184..15297
                     /locus_tag="PK937_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31578.1"
                     /translation="MIKSVITYCGLTFLFVFGAAIYSQNMVKGLPVIEHYAPQTYGAG
                     VDNFCFVQDKRGFIYVANGSGVLEYDGIQWRLIPLENRAAASWLTISPEGTIFVASLG
                     EFGCLVPDSSGFLKYQSLVNRQTHPQLKFQTFWEMCASSQATYLRSYRHLVRYKNGNI
                     KVWSADGDGFDIMGMVYDTIYTRIKHETIVRIIGDSLVPLPGGDFFKEIKINGFLPYQ
                     KNKLLIATRKDGLFIYEAGQIKSLKTEADDYLKKYFVYDTRQLPDGGYVIATLQGGVV
                     LFDADLRVKQIIDETSGLPANGVNSVFVDNNFNLWLGHSYHGITRIEIHTPLTLLNAS
                     TGINGRVKRMLRHKGTLYVMTNSGLFRLTEKGNAYHFELLHSGAPYYFDMFVTQGRLL
                     ISTGSGILEVKNDNVVSHTSVYTYAVLNSNRDSNIVYASEGLGMTIYRVGKSEKWELV
                     KTIPSVKVAVRTMAYDEYGDLWLGTEYEGLGRMQINKKDHLPDTVEFFDSKFGLPAGE
                     ISVWSTGAAPLFSGHDGFYKFSRQYHRFNKLSETEMAMHRDNSEYWIGLNQGRWMITP
                     TALQIMPEKTIINRLRAIKNAVDFIYWRDSDGTEWYGGDFLIVKHKPKTQTLLPERPT
                     VVLRRVMQGLEVIFSDDEIKRNHQIVFESEPTQPIRFEFTAPVFEMRPQFQTRIIGLN
                     EQWESWGSDPVRLYERIPAGKYRFEVRARDHFGRISEITGADFVVNPPWYLARTMLML
                     FALVAIALVWLFIRYREHRLKQMNQELERLVEDRTRALKAKEAQLIQSEKMAALGQMV
                     GGVMHEINNPLTFIQPNLEYVQLNLKKFISDITANAVTENRQAEIREFERDTINALKS
                     SVKGAERIRDIVSSLKSFSAYGKNQVMDVRLDHQLSGVVDLIFNQNNDIRFIRSFKDT
                     RFVRIDTMEVMQCVVGVLNNAVAAVHDALAEKILLHENAEIRICTMDGPDQTVIIEIE
                     DNGIGIPETVLPRIFEPFFTTRMIGKGKGLGLSEAYAVIKKHHGNIEVTSKPQKGTRV
                     IITLPAN"
     gene            15404..16531
                     /locus_tag="PK937_03055"
     CDS             15404..16531
                     /locus_tag="PK937_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH31579.1"
                     /translation="MTWILLFICFVCSDSIAQTLTFEEYEPPSTLVVPENFRYQSKYP
                     FIDVHNHQFEMPTQDLKYLTKQMDSLNMAVMVNLSGRGFKREQNKDGSFRFGLQDGSY
                     LKGAIENARKNVPGRLMVFTNINFGGIDDPGWSAEAVRQLEEDVKNGACGLKIYKNLG
                     MSFKDSSGNIIAVDDPRIDPIWKKAGELGIPVLIHSADPFQFWQPWDKNNERWLELKE
                     RPDRKRDSSKEPSFESIIAAQHRVFKKHSKTIFINAHLGWMGNDLAKLGKLMDSLPNM
                     YTEIGAVLAEIGRQPRQARDWFIKYQDRVLFGKDSWNPQEYSTYFRVLETNDEYFDYY
                     RRRHAYWKMYGLGLPDDVLKKLYYKNALRIIPGIDKSLFPK"
     gene            16765..17376
                     /locus_tag="PK937_03060"
     CDS             16765..17376
                     /locus_tag="PK937_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31580.1"
                     /translation="MRRTMLLLFVCIATMAHAQDLPEIDGFIREGEWSDATVFSDFHL
                     ISPRSTEKYYDSTIVYIKQSSDALFFAIRFWPKGRVISQSFVRDRSSDEENEFFILLD
                     LENKRQNGYFFAFNFLGNQRDMRMYNQRQMSQEWDWNWQNKSTIFEDATPDKPGYIET
                     EVRIPVDKLQNKSPNTIGIDVQLFAYKQDGTSYFYRSALKVNS"
     gene            17436..18764
                     /locus_tag="PK937_03065"
     CDS             17436..18764
                     /locus_tag="PK937_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31581.1"
                     /translation="MSAIPYAVGYTFTDSSYEARVGGEFSVSIDRHTLKGTYNTDAST
                     LEADPFVFSFYKRKVSLAEKRPFFSRDLDVYRSPISLLYTRGIADINYGANYTFRSNT
                     LKAGAFYVEEEPIPGDTTHNIRRFMAVRPNVVTKFVNFGGTFIHNEQTNGPSETIISG
                     DARVDLPSRWVFVPQLATNRDGYASKAYLYYEFNPAGGPYADFSYTRFGKKFDGMTVI
                     NLFGKNYDEISNSVGYNFIYNRSYFHQMNARVEFYKAESLDRDFMLQRNINGNFFYKV
                     NDWLSVNHYAEISNADEALSDTTKKNHNDYAQDHNVKILIGNNAIVLGYKEGSYYGQK
                     LRNPYASYESMFFSRVGVSATAFLVENEARIFSAKINYRIMPKLFLRSLYQWKSEKGK
                     SNKYDNFQLWNSMLQYEFFAGSNLYLVLNLQGEDLEYVGRYCKLAYEFNF"
     gene            complement(18768..19160)
                     /locus_tag="PK937_03070"
     CDS             complement(18768..19160)
                     /locus_tag="PK937_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31582.1"
                     /translation="MSVRWLSGPMLPEIVWKKFSATGAFRTAFDVALQHVKNRRSVAL
                     HADFELLTVLDSDDQAWLRNEWLSKAAQFGLKHIGVVLPVASIGKTSLMKGLRAPDGN
                     ILEIGYFMHREQSRDWILRMSRMHRSSQ"
     gene            19442..22540
                     /locus_tag="PK937_03075"
     CDS             19442..22540
                     /locus_tag="PK937_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNH31583.1"
                     /translation="MTALSILIICSDYTTAQNFEKDVAAAFRWRMIGPFRGGRTVGAV
                     GVRQQPNVFYIGVNNGGVWKTEDFGRTWFPIFDDQPTGSIGDIVVAPSDPNILYVGSG
                     EGLQRPDLSVGDGVYKSTDAGKTWKHTGLRDGQQIGGLAIDPENPDRVFAAVLGHPYG
                     ANTERGVFRTINGGSSWEKVLFIDENTGAIQVTLDPKDTKTVYATLWAARQGPWENGS
                     WQGPASGLYKSTDGGTTWKKLTQGLPTTKEGLGRIGFCIAPSLSSRLYATVDSPEKGG
                     LYRSDDGGNSWRLMNPDNRLWGRGGDFAEVKVDPRNPDIVYSANVAFWKSTDGGKNWT
                     CVRGAPGGDDYHRIWINPDNPDIMLLAADQGAIITVNGGKTYSSWYNQPTAQFYHVST
                     DNAFPYNVLGGQQESGSASVSSRGHGGQITYRDWLPVGVEEWGYVAADPLNPDLVYGG
                     KLTKYDRKTGHTQSVGPFPTGHYQPNRSPYRFLRTAPVIFSQADPTALYYAGNVLFKS
                     QDGGHAWRIISPDLSRESWDVPKSIGVYTTESMKTMARRGVIYTVAPSPLDSSVIWAG
                     TDDGLIHITRDGGKTWQNVTPAEITSWSKISLIDAGHFDVNTAYVAVNRIRLDDMKPY
                     IYKTHDGGKTWIKIVEGLPDDPINAVREDHIKEGLLICGSERAVYASVNDGQNWLSLR
                     NNMPATSIRDIVIKDNDIVAGTHGRSFWIMDDIMPLRHITEVSRQAPVWLFKPSNAYR
                     VRWNLYTDTPLPQEEPAGENPPDGAIIYYHLKQDAQTPIVIEIKNSRGEVVRRFSSND
                     SIPMSQDNNFPSYWLAPPQRVSAKAGSHRLIWDLRHNPLPTEPEFSIAAIYGKTTPAP
                     TSPLVMPGFYSVVISVNGLTMGEGLTLVMDPRVRMTDTEIKDQFDLSMRCYEIRKKGM
                     LVTQHIQSWRVQADIYMGQIKSNSSEIKKFLGYLDAIEKGNTATGDKGLQTILQSATN
                     MFDVIQHADMPPTAAMYNTVRETEKSWEKLTAEWEKLKTVYLNDLNAHLKKNKLPEIS
                     NP"
     gene            22589..25861
                     /locus_tag="PK937_03080"
     CDS             22589..25861
                     /locus_tag="PK937_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNH31584.1"
                     /translation="MNLRAWLMLCSLLISTHVVAQKKNAETKDPMPSSTFGGLKFRSV
                     GPALTSGRVVDFAVNPKNIFEYYVAVASGGVWKTTNAGVTYEPVFDGEGSYSIGCVAI
                     DPSNPNVVWVGSGENNNQRAVAYGDGIYKSVDGGKSWTNMGLKNSEHIGRIAIDPNNS
                     DIVYVAAYGPVWSAGGDRGIYKTTDGGKTWKAVLTVSENTGFNEVIMDPRNSNVLYAA
                     AHQRRRSVFTYIGGGPESALYKSTDGGANWRKINSGLPAVDLGRIGIAISPINPDVLY
                     AIVEAAEGKSGVFRSDNRGESWEKRGNNSSSGNYYQEIFCDPQNIDKVYYVDLFVMLS
                     LDGGKSFNPIGEKYKHVDNHALWINPANTGHMLVGCDGGIYETYDNGANWEYKWNLPV
                     TQFYKVATDNATPFYYIYGGTQDNNSIGGPSRTLSANGIINTDWFITNEGDGFESQID
                     PFDANIVYAQSQYGGLVRYDKKSGEVIDIKPVEGENESGLRWNWDAPLLVSRHKANRL
                     YFGANILFRSDDRGHSWKAISKDMSRGLDRNKLSVMGRVWSIDAVAKNGSTDIYGQLV
                     SIAESPLDENVLYTGTDDGLIHVSRDGGASWTVVDNIPGVPERTYVNQIIASQHDKGT
                     VYAIFNHHRYGDFKPYIFRSRDFGKTWSAIQNNLPERGSVYCVGEDHMRADLLFVGTE
                     FGVFFTIDGGQKWVQLKSGLPTIAVRDLEIQRRENDLVLGTFGRGFYVLDDYTPLRHL
                     KKEDLSKEAMLYPVKDALMFIESRPLGLSEKSYMGENLFSTPNPTVGAVFTYYLAEDI
                     KTVKEKRRASEKEKASKSESVFYPSMDSLKTEELQTEPTLIFTIKDDAGRIVRRLKAP
                     AKKGMNRITWDFRYPSPGPVTFNVPVSYNFYDVLEKGHVAMPGNYSVTMGKIEDGKYT
                     ELTPAQSFKAVALNASALSATDKNAFDSFAKSVSDWRRQISSADAYRGELAGKIKFMK
                     AAALDAVEPGSEIIEAVYTVERRLKEFNLKLNGDNIRDKYQFERAPSVSDRIGQIEFS
                     IWNATAAPTETQKQSLAVVAKQTPALMDELKSIDTEIKRIESMLERGKAPYTPGRMPE
                     KK"
     gene            26049..29279
                     /locus_tag="PK937_03085"
     CDS             26049..29279
                     /locus_tag="PK937_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31585.1"
                     /translation="MHALYAQNEEGLIPTQYYSTSQFFAHNQNWAVTQDQQTGLIYAG
                     NNVGILEFDGATWRIIHTANRTMVRSLAYDPVGHRVLVGAQGEFGYLKPDSVGIMRYE
                     SLSAVLPEEARRFVNVWRIWVTNRGTFFQTNTEVFILQNETLRIIKPDQSSFHFSFLA
                     NGNVYVTLRNVGLQKFNGDKFETVPGGDRFHKIPIYAVLSYAPDTVLIATQEGLFLGL
                     NNGVIPFGETSKPFLKTNRVYNGLNLPDGRFALATLTGGVAILNRNGTIARVLDKSNG
                     LGDNAAKSIFLDRDKALWVSLEKGICRIEYFSPFTYFDDRVGLLSQFETFVRWKGKFY
                     IGTKDALYVLDRQPSSHIPYHFKTLYDVPEEHWSLLPMGDVMLDATFRNGIYEISSHG
                     RKRISPNSTAFLLRSRLDTHRVYAALWDGLSSVYLRNGIWVDEGRYAGITDEVRVIAE
                     DSDGSLWMDQKTGGCARMSRINDSIYVRRFSVKDGLPNLTINPYFFFGRMWFATDNGA
                     LYRYDEKASRFYPDTTLHHRLGIATSKMISPIGETSDGTLWFALGNDESMRFEPAYAV
                     PDGDKYRVTILAHGRLKIKLSPGNFFVEENAVWFYGHDNFYRVDRLRANGLSHDFKVN
                     IRKFTLNDSLLYGGALHSDLPTIVVPFGTSVIRIDYAATAFENQNDIHFKFKLDGFDE
                     EWSARTTEAFKEYTHLPEGKYTFRVKGYNVYEFESSEASIDFIVLPPWYRTWWSFVLY
                     FFVGAGIVYAGFRWRVRLLRLRNKELFVKVEERTRELASANHEIETQRDFLKNMNQQL
                     ESALKDLRETQTQLIQTEKMATVGQMVAGVMHEINNPLTFILPNIEYIEKSWKKLVTY
                     TERSKPHVAGAQDPELLAIIQDIERGLLIDETFGALDSMRRGSQRIKQVVVNLRAFAA
                     MDYLPKSETVVDAHIEQTVDLFFGTVKDLSFVKKLSYPTPIKVRIQEFNQSVVALLNN
                     AVQAIRDAEMQGTLERGKGVIEIRTQSHKGDANTLEFSISDNGIGIPEDILPRIFEPF
                     FTTRSVGQGRGLGLSEVYAVVHKHNGLITTQSKVGHGTTLTITLPA"
     gene            complement(29365..30435)
                     /locus_tag="PK937_03090"
     CDS             complement(29365..30435)
                     /locus_tag="PK937_03090"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNH31586.1"
                     /translation="MKTLLCFVILACGMMTSAQDLRFPDASPEAMIMQEVGISKVTIS
                     YSSPAVNGREIWGKLVPYGMEKSNPFGHGKPMPWRAGANENTTITLTHDAKIEGQTLK
                     AGTYSIHMIPEADQWVIIFNNNYTAWGSFFYEESEDALRVTVKPQMMADSQDRLSYGF
                     DNTDRNKTIAYLRWEKIKVPFTISFDTPQIVLNYIRQRLKNREAWDHGSLSQAAQFAL
                     RNNSLDEALSWTDRAINSGGGTSAFFLKSIILDKQGKKTEAEAVKKRAIDNATEADLN
                     TFGYQLLGQGKATEAVEVFRANAKKFPESWNVYDSLGDGLEAIGDKKGAIESFTKAQK
                     LVKDDANKRRIGDTLKRLQDSK"
     gene            30619..31692
                     /locus_tag="PK937_03095"
     CDS             30619..31692
                     /locus_tag="PK937_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH31587.1"
                     /translation="MILIAGVSIAVFIAALLISKKQKSQGDIILTAWMMIIAVQMLFY
                     HAYITDIIFRYPIFLAWERPLPLLHGVLLYWYVATMTDQRPSSKFLLYLHFIPTLFFY
                     LVLIPFFLRPVEEKIWVYRNEGAGYEILDAIRMGAIILSGTIYIIWSAILLSRHKRSI
                     LNRFSYEEKINLQWLRILTWGLGSVWLLIFISDEVLSVGVTIFVFMIGFFGIRQSRIF
                     KTTENNLEVPEKEKYAKSGLTKETAETLYAGLMRLMNEEAVYQKNDLSIYDLATRLNV
                     HPNYLSQVINELERKNFYDFVNQYRLNHFIKLISDTRNRYLTFLALAYESGFNSKSSF
                     NRYFKKATGKTPSEYFSSEGLVN"
     gene            31738..32328
                     /locus_tag="PK937_03100"
     CDS             31738..32328
                     /locus_tag="PK937_03100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNH31588.1"
                     /translation="MTSFLLYIIFVYLVGGLCGAYYLLHWKTGENLRDHGSGNLGATN
                     AGRVLGKTGFIITFLWDAGKGSAVIYGARILDYDQHLIWICAIAVVTGHIWPIQLQFK
                     GGKGAATLIGILVALDAYLLWPLLFIFLASFVILRRFGIAGALAFFTLPLVVWLRGYE
                     PLSVILSIILTIIVIFAYRDHIVTFVSAFKKRSRNK"
     gene            32377..33282
                     /locus_tag="PK937_03105"
     CDS             32377..33282
                     /locus_tag="PK937_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNH31589.1"
                     /translation="MSIRPVKRIIQSTPTMEGAGVRLRRAFGFGNTEDYDPFLMLDDF
                     RNDNPNDYMAGFPWHPHRGIETITYVLAGNVDHKDSLGNAGSLGAGDVQWMTAGSGIL
                     HQEMPQGDAQGRMHGFQLWANLPSSLKMTGPRYQDVKSKDIPEISEDEGTKIRVVCGD
                     YGGQKGPVDGIAADPRYLDVFVPSGKRKTLKVETYRQAFAYIFEGSGSFRDASQPHGI
                     LTEAPDDHDNVSRSMAGNRSLVLFDSGDEVTVQAGDEGIRFLLVSGRPIKEPVAWYGP
                     IVMNTNAELQQAVSELRNGTFIKHG"
     gene            33956..34519
                     /locus_tag="PK937_03110"
     CDS             33956..34519
                     /locus_tag="PK937_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH31590.1"
                     /translation="MARTPSNMASLGTFAPDFDLPDVISEKKLKLADFKEKPALLVMF
                     ISRHCPFVVHVEKELARIGKDYPQIGIVAICSNDIENYPEDAPDQLREQANRLQFTFP
                     YLYDASQETAKNYKAACTPDFFLYDQSRRLIYRGQLDDSRPNSVIPVTGRDLRNAIEA
                     VLSGHRPSTEQRPSLGCNIKWKPGNEP"
     gene            34585..34830
                     /locus_tag="PK937_03115"
     CDS             34585..34830
                     /locus_tag="PK937_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31591.1"
                     /translation="MFDWFKKKKKLNPKPKQSTNFDSGTMYDPLSPTYVTAGLASGAI
                     EEETVDDRNIEDSSNSDDTPESSSDASDSGSDSGGSD"
     gene            34920..35468
                     /locus_tag="PK937_03120"
     CDS             34920..35468
                     /locus_tag="PK937_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH31592.1"
                     /translation="MAVRSKKAVSKKNVARKPAKKKQIKKKVHFIPKGFHTLTPYLSV
                     HDGKAAVEFYEKAFGAKERKGRMSDEKGKIMHTEIKIGVSHFMLSDEFPEYGNVSPKT
                     VGKSPVQLMIYVPNVDILFEKAIAAGATVVMAVEDQFYGDRSGRLLDPFGYQWHIATH
                     MENVSPKEMARRAAKLYGAQAS"
     gene            35484..36050
                     /locus_tag="PK937_03125"
     CDS             35484..36050
                     /locus_tag="PK937_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31593.1"
                     /translation="MKYKSCTTDGELLQILDLQKRNLPDLLTNEERQSQGFVTVKHDF
                     ETLEAMNTPFAHSLAISDDRVVGYALTMERSMEARIPILQPMFTTLDTLSIQNRKMSD
                     EGYIVMGQICVEKAFRGKGIFRGLYSHMRSRLGPYFRYLITEISERNVRSLNAHSAVG
                     FQKLITYEAPDGERWVIVGWNWNQSNTK"
     gene            36063..36479
                     /locus_tag="PK937_03130"
     CDS             36063..36479
                     /locus_tag="PK937_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH31594.1"
                     /translation="MAENKTKATKLSVQDFLKTIKDEQTVKDCNVLIKLMKKITGKPA
                     VMWGPSIIGFDSYHYKYESGREGDMCITGFSPRKGKISIYITSGFERYTAHLKKIGKC
                     KTSKACLYVKSLTDIDTQVLEEMIKDSIIFMKKKWK"
     gene            36676..37485
                     /locus_tag="PK937_03135"
     CDS             36676..37485
                     /locus_tag="PK937_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31595.1"
                     /translation="MKHVKTVILLLVFFSSFVFSHGGGKNVKLHVNPKWKQCSIQLDS
                     ALNQSAWRQFTREAGLVSYFRPLTDAKPMGKGNYEFSLLQWQSKFDDTDDAWNHTFVH
                     PDSTHWLKENDRLPFPGLTFRLGISEKMDLAAYWTSSPGANYGFFGAQVQYNIVQNWL
                     NQWSASARLSFVSLYGPKDLDLSVYGLDVIASRDFTVYPKWLKVTPYVGLSSYMTDSH
                     EKSSKLNLHDETAWGVQSMFGGVISISKARLAAEYNISNISTFSVKLGIVF"
     gene            37734..38153
                     /locus_tag="PK937_03140"
     CDS             37734..38153
                     /locus_tag="PK937_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31596.1"
                     /translation="MRTLTLVIISCFALSCESINQSLLNHDDGSEKNFLSYEVTLINN
                     DGNYSRSGVLRIQWNNEGSITGQWIINQNESYEVKGQKQDSGFIIELFPYGIDSGESI
                     SGSFSTDKQTISGDWEQMTIAGPLTGSFNGFSTDIHP"
     gene            38354..39082
                     /locus_tag="PK937_03145"
     CDS             38354..39082
                     /locus_tag="PK937_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31597.1"
                     /translation="MDTPVALVTGSGKRLGRKIALALAETGYDIIINYHQSVVDARKT
                     EKLIRQIGRKAVLIKADVRRPDHIKKMFNEVIKKFGKIDVLINNAAIFPKKTELPNIP
                     ESIWDDVIDSNLKSSFLCAQEAAKYMLRQKSGKIINIASLGAFLSWKNYIPYNVSKAG
                     VVMLTRSLAKALAPDITVNAVAPGTIIVPEEEVLSEHLPTMDRIPMKKYGKPADITAA
                     VLFLLQNEYITGQIVGVDGGATIP"
     gene            39167..39238
                     /locus_tag="PK937_03150"
     tRNA            39167..39238
                     /locus_tag="PK937_03150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39199..39201,aa:Thr,seq:cgt)
     gene            39799..40950
                     /locus_tag="PK937_03155"
     CDS             39799..40950
                     /locus_tag="PK937_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNH31598.1"
                     /translation="MELNAECPAGIAYSDIAAKVYDKLPMIREFKKHFNVEKFTIRQE
                     LLNGLLSTYAVWRGNKKNKKPRIAIVDWREVPTFTEFQLFQEFFKSKKLDCIIVDPRD
                     LTYEKGRLMAGKKEIDLVYKRILTNDCVEKPEETKALVQAYRDQNVCMINPFRAKLVH
                     KKSMFAVLTHEKNQKLFNGEELSVVLKHIPWTRMLRDEITYFNGKQIDLLDYVSTYKN
                     NFVIKPNDEYGGKGVCLGMEATDQQWSAVLHEAKNGEHYVVQELVKIPKQPFPTVING
                     NLEFVDMVVDMDPYAFGPNVEGILTRLSASSLANVTAGGGTTPTFVITKKTKKAKKTL
                     KNEIRRFISKQKPKLSYLKTNNKKTKKKVTVKRRSKKIEMINKNKYLII"
     gene            40947..42059
                     /locus_tag="PK937_03160"
     CDS             40947..42059
                     /locus_tag="PK937_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNH31599.1"
                     /translation="MNKPAFTIGIEEEFQTVDPETRELRSHVSGQMIEEGKLLLSEQI
                     KMEMHQSVVEVGTNICQNITEAKADVIKLRRTIHQLATKRGLGIVAASTHPFSDWKTQ
                     PITEHERYKEIVENMGDVARANLIFGLHVHIGMPDNESCIAIQNQIRYFLPHILALST
                     SSPFWIGRSTGLSSIRTQIFRRFPRTDIPPIFDSWSDFEDYVNLLIQTKCIDNGKRIW
                     WDARPHPSFGTLEVRICDLPTRVDETIAIAALIQALMHTLYRTYKKNMSWRIYPNQLI
                     SENKWRASRYGLHGKLIDFGKKEEVPTKELIYELIRFVDDSVEELGSRNEINYIYKIL
                     DGGTSSDRQLAVYEQSGRDLKAVVDFLLRDTLEGLY"
     gene            42113..42775
                     /locus_tag="PK937_03165"
     CDS             42113..42775
                     /locus_tag="PK937_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNH31600.1"
                     /translation="MGIFTRMADIVKANINDFLDKAEDPEKMIKQMVIEMEEAVNKAT
                     TAVGTAIANQKRLEKQFNENKKLADEWQNKAVQAVNAGRDDLASQALAKKNSYATAAA
                     SLEPTLQQATATAEQMKTQLQQLKAKLDEARVKQNTLIARHQAAKAKKMIAQQMSGIG
                     GGAFGNFDKFEKKVEDVEAEADAHVELSGQNTSLEDEFKKLSTDTTVDSELAALKAQL
                     GK"
     gene            complement(42850..43335)
                     /locus_tag="PK937_03170"
     CDS             complement(42850..43335)
                     /locus_tag="PK937_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNH31601.1"
                     /translation="MNTPNGYAEIVKQFGDIRKYILSNGTLSSQFEMKYMGNAVLPYP
                     LKLAWMPEKTIKRMYCHRDMIPVFQKVFNDIYQAGLAPKAELFGGCFNFRLKRTTNKL
                     STHSWGIAIDLNPATNALGTKGNMNPSIVKIFKNNGFIWGGDWADLNCDPMHFQFCDG
                     Y"
     gene            complement(43617..45086)
                     /locus_tag="PK937_03175"
     CDS             complement(43617..45086)
                     /locus_tag="PK937_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31602.1"
                     /translation="MISFLEKITGFHESELASFFDSNPNARNEFHRIANDQNRKNAVV
                     FIWFLILTQFIVGIVRIISGWGGVPYYNLALIGLGALLLFLFRYRSFRQNVTHISLIL
                     FTVWAITFPFFIIRLGGYEAPTYPVYIVIAVYILAFFNYTFRQYLYLYVIIFLSNACI
                     YFLETDVETDSYVYRQSVMLFLYMVALSGAYIHVHLRKKSFQVQYELGQRKSELEKAY
                     KQLEEKELQLVQKEKLASLGTMTAGIAHEINNPLTFIDGNLQSLAEYINDLKTLVSVW
                     DKNFDTVDQQKQYVNSIKAQIDYQFLINDLDKLLASCNNGSKRIVDIVNKLKNFSNLD
                     SAEFKTVNLCDSLETMIYLLKSQYTDVKFNFSKKCDQTESAELACNPGLLNQAIMGII
                     ENGIDAARLNKSETPKIIISLFANPEEININITDNGLGISKEVQEKMFDPFFTTKDVG
                     KGSGLGLSVAFGIIKQINGRLEFKTTQGRGTEFIIKIKK"
     gene            45242..>45452
                     /locus_tag="PK937_03180"
     CDS             45242..>45452
                     /locus_tag="PK937_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31603.1"
                     /translation="MEQVGINSNVYAGGKIFHIQTAGSYNDLVVQSEIFEAGRIISVQ
                     KKRITDVTEMPEDDFREFVKKYHHEV"
BASE COUNT        13547 a         9277 c         9736 g        12892 t
ORIGIN      
        1 gtaaagccga taaagccata aagagaagcc agcgtttcat atacgattcc ataggataat
       61 tatgcattca gtttggctcc gcattcggag caaaatttag ccgttttcat gttttcatgt
      121 ttgcaagaag gacatattgc tttttgcaca acgatggttt tggcgcgtcg ggattgtatg
      181 agatgctcca ggtattcgtc cgatattttt ccattaccgt tgtcgccttg ttcgatggca
      241 tccattcgtt tgagaacatc ggcgagttct ttgagcgtag cttgacgcaa gttttggtaa
      301 tcggcctcgg cgattttgcc gataccgtac tcaaaatcca aatccttgat gtcggtataa
      361 agtttttgct tcatattagc cagatcgtcg cgatgctgat ccttaatcgg tttgagtccg
      421 taaggcatca catttttcga aaaaagcgga tacagtacat atccgaaagt tgcggcagaa
      481 agtaaaatca tcaataacga taacataaat caccgatcca tttttttgag ttcttcttcg
      541 atacgttgcg tgtacgggtc gtcggcggga tgggccggtg aaaccgtaac cggagccgtt
      601 ttaccggccg taattttttt tatgagaaaa accacaacga tgataaatat aacgaacaac
      661 agaaaaggca tgatccacgc cagaagatca aatcctttcg tttcaggttg tgaaagaata
      721 gcttttcccg ccgggggatt gtcgcgaaac gaatattcgg tcataaaata ttggatgatt
      781 gcatcgtggg atttacctgc aacgatcatg tcttcgatcg tattgcgcat cggcacggcc
      841 gaagggcagt tgatatgccc gcaagtcccg agcaccagat tgcaaccgca ttggcaaatc
      901 agttcgcccg atatcttatt aaacatttca ttttgttcgg cattaagttt agtcgtgtgt
      961 gccgacccgc cttgggaata aagtgcggat gttattaaaa tcgaagcgat aaaataaatc
     1021 ttaagctttt tcaagcgtct tttcctttcg catagatttg acggatttgc gatcttcctg
     1081 gtaagcgggg agcaaacaaa tcaaagttcc aaaaacaagt atctcagtgc caagccaaac
     1141 caatgaaact aacggattga cataaatctg taaaacagct tttttctcgg tatcatccag
     1201 cccggtaaaa acgagatata aatcttcttt gagtgttttg tgtaacgcca cttccgtcgt
     1261 aggttgttga ctggagcgat acatacgata ctcgggtttc aattcatcaa tcttcttatc
     1321 atctttatat acggaaacga ccgcataaga ggattgatag ttcggcgtat tggtgaagtc
     1381 cactttatca atcttcaaaa ggtaatcttt cacctgccgg gattcgccct gacccagttc
     1441 cgttttggtt tcatagttaa aaatgctgcc cgtaatacct acgaaaatca cgacaatgcc
     1501 gaagtgtacg atgtagcctc cgtaacggcg cttgtttttg agggttaagt ttttgagtgc
     1561 gattaacacg ttttcaccat tggaacgcat acgcgcactt gttcctttgg caaactcaat
     1621 cgcaataacc gccgcaacaa aaatattgag cacaaacgac attaatccgt aaaaatgctg
     1681 cgtaacaaac ataaatatga caacaccgcc ggccaaagca atgatcgtgg gtaataagaa
     1741 agcttttcgc aaactcgctg ctgaagtctt acgccatgca aagagcggtc ccgcgccggt
     1801 aaggaacaga agaaataatg ctatcggaat atttactttg ttgaaaaacg gcgcgcctac
     1861 gacaattttt tcaccggtga cggcttctga aatgataggg aataccgtac cccacaatac
     1921 agcaaaacac gaaattagaa gtacgaggtt attaaacaaa aaactacttt cacgcgataa
     1981 tgccgaatca agctgacctt cgctcttgag cattggcaaa cgcttgatca caaaccaaac
     2041 cgaaaccaat aaggacaatg tgatgaagga aataaaatag attcctatcg aaccttgcgc
     2101 aaaggcgtgt acagacgaaa caacgccgga acgggtgagg aatgtaccga aaatacacag
     2161 aaggtacgtg gcaatgatca aagaaacgtt ccatactttg aacatattct ttttttcctg
     2221 aatcatcacc gaatgtaaaa aagccgttcc tgtaagccag ggcatcagcg atgcattttc
     2281 aaccggatcc caagcccaat atccgcccca accgagctct tcgtaagccc accaaccgcc
     2341 catcatgatg ccggcgcttt gaaaaagcca cgcaaaaaga gtccagcggc gcgatagttt
     2401 gatccattcg tcattgagtt gtccggaaat caaagccgcc atagcaaaag caaacggtat
     2461 cgcaaagccg acataaccca caaaaagtgt aggtgggtgg atcaccatcg cgggatgctg
     2521 caaaagcgga tttaaaccac gaccgtcctg cgggcgaaac aaggtttcgc tcccgtcgcc
     2581 atgatgcgtc acaaggacat caaatgggtt ggaagaaaaa agattgagag ccagaaaaaa
     2641 aacttgtgtg gccatcgtaa cgacgataac ccacggcatg agttcgccga aactattgcg
     2701 gtatcggtac acgacggcaa aactatatgc cgcaagcaac agcgaccaca aaagcagtga
     2761 tccggcctga cccgcccaaa atgtatattt gtaaaaaagc ggcaagtccc ggttagtgta
     2821 cgatgcgaca tattcgtatg tcatatcacc ggtaaccatc aaataaatca aacttccggc
     2881 ggcaacgaca accattgcaa aaacgacata cacggcgttt tgtgccgaac gtaccaaatc
     2941 accgcgcttg taatgaacgc cgagaaacgc cgacaagatg gcaaaagcag ccatcgccag
     3001 cgctacgatc aacgcataat tacctatttg acccatatat attccttagc ttatttgaca
     3061 gacgatgtat acgctatttt tattaacttt tccatagaat ttcatacaca gttaccggtt
     3121 cggcaacgcc tttgagcgta acgcgttcga tcggcgaggc gatgctttta ttgggcaaaa
     3181 gatcatacgt ggactgactg acgatgatct gtccggcttt agcgacgctg cacaaacgat
     3241 tggcaatgtt gacggtttca ccgatcacgg tatattcaag gcgtttattg gagccgatat
     3301 tgcctacaac gacctcgccg gtattgatgc cgataccgat attgatcggc ggatgacctt
     3361 ctaatttgcg ttgaaaatta aatttctcag ccgcgcgttg catctgtacg gcggcattga
     3421 tggcgcgcat ataaaaatca tcgtagtgaa ccggcgctcc gtaaaatgcc atgatagcat
     3481 ccccgataaa tttatccaac gtaccaccat aactgtcaat aatgtctatc atttcatcaa
     3541 agtgcgaatt gagcaaatta ataagttcct gtgggtccat accgcgcgac atcggcgtaa
     3601 atccgcgaat gtcggtaaac ataacgctga gccgattctt ttcgcctttg agaataccat
     3661 caacggattg ttcggacaaa acttttttgg caacgtcagg ggaaagatat ttaccgaaaa
     3721 gattttcgac acgttctttt tgcttgagtc cttgcgtcat ctcgttaaaa tttttaccca
     3781 gttcgccgaa ctcatcgcgc gatttgatct gcaacatgat attgagatca ccggattgta
     3841 ctttgtgcat agcgtcggaa agctgatgta caggcttggt cacgccgctg ataatcacca
     3901 aagcaatcat aaaagccaaa atggtaacac cgccaccgac aaaaaccatc gtgcgctgcg
     3961 aagcgacgag cggctctaag gctttgtcga gtgaactaaa aaaaacatac gttgcaataa
     4021 ccgggcgctc ctgaccctgg cttcccgtag ctgcaaaaac aggatgggcc acagccaaat
     4081 aacgctcacc atgaatcgta aactgaaaag gatcgcttga ggttttacga atcatcgcct
     4141 gatctaaaaa actttgggat gatttgaatt gggcgagaag ttcatttcgg ttgtcaaact
     4201 tatcggttgc aacaatcttc ccgttgataa taaaggcaat cgaactggtt gtaacttttg
     4261 caattttggt tgctacggta tccgtcacat catatcccaa aacgataccg ccaagaactt
     4321 gattattaac gatcgtaggg gaagccgcca cacggagaag tttaccattc tcttcccaaa
     4381 aaccaccgtt atcatagcct tcgagcgcgg cggcgacttc agggcgctgt gaaagattca
     4441 tactcgcgct cagctccgtg ccgacaaatg ccaatacctg accggacggg ctcaatgcga
     4501 caaatatatc ggatttgaat tcgccgattt gattagtttc ttccagcgct tgctgaactg
     4561 ttttttggtc acccgtacta agttgtgcag cgaacttggg attatctgaa acgctacgac
     4621 tctggagtat caattgttcg aaatagctgt tattaaattc ctcataggtg ccgttacttt
     4681 tgattagccg atcggtaata tcggtttcga tatttttagc caaaccgagc gaaacgatga
     4741 cgtacgcaac ggcggccagt gtcaaggtca cagtaacgag aaaaatcagc ttgtaacgga
     4801 atttgataat catgttttat ttctccggac ggttaatcac atgtggtttc aaattacctt
     4861 ttgggtattg aaaatacaag cgttatcgta ttcgttcgcc tgtcgtgatt ttagagtact
     4921 tttcatccgg aattgtgttg attcgtgtta cattcgcttc tatcttaaac aaacgaaacg
     4981 gataaaagta tgactttgat catagatgcg catgggcaaa atctacaatt cgataaaccg
     5041 ccggaacggg ttatttcact gataccgagc attacggagt cgcttttcga actcggactt
     5101 ggcgatcgta tcgtagggat ttcaaaatat tgcatctacc cggaagacgg tgtccggaat
     5161 aaacccaaag tcgggggaca gaaaaatcct gattttgata aaattactca attgaacccg
     5221 gatttgataa ttcttaacga agaagagaat aaaccggagc atattgcgtt atttaaaaaa
     5281 aactatagga cttttgttac atttccgcgt atatttgtcc aagcagggaa gttacttgat
     5341 cagttggggt taatctttca tatctccgac cgcacaaaag ttttctctga acaaatacga
     5401 ctcgatcata gtcggctgac agtcttaagt cccacacggc tccgggcctt atatcttatc
     5461 cggcgcaatc cgtggatgtc tgtcaaccgc gatactttca tacatgatat tctgtctttg
     5521 gttggtttgg acaacgtttt tgcaaaccaa tctccgcgtt attttaaagt tacgcgggaa
     5581 atgattttga atgcacagcc ggatgttatc attttaccgg atgagccgta ccgttttttg
     5641 aaaaaacata ttgcagattt cgacggtttg gacattccgg ccgtaaaaaa ttgtaatatt
     5701 tttctgacgg acggtactta ctactgttgg tacggtacgc gaacggcccg agcaataaag
     5761 tatatagacc aaaacatcat acatcggatt gttacatgct aaaacttctc ttcgtttttg
     5821 tgacgctatt atccctctca ttacaggcgc aatattacga taattatttt ggcaccaata
     5881 aagtgcagta cacgcatttt gagtggaata ttttagaaac ggaacatttt gagatctatt
     5941 attatccgga aatgcgcgaa ctcgctgaac gtgccggcgg gttggctgaa gagcaatatg
     6001 cggagcttca atcccggttt aatcatgctc tgattaaaaa agtgccgatg attatttata
     6061 gttcgcacat gcattttcaa caaacgaata cgatatctgc cgaattaccc gaaggcatag
     6121 gtggtttttt tgaatttttc aaaggtcggg tcgtattgcc gagtgacggt tccaccacgc
     6181 actttcgcag ggttttaagg cacgaattgg ttcacgtttt tatgaattcg aaggtgaacc
     6241 ggattatcag tcagcatcga aaatttaatc atcccggccc ccctttgtgg ttcaccgaag
     6301 gtttagcgga atactggtcg ggtcaacccg attttcaggc tgagatggtc atacgcgatg
     6361 cgacgctcaa cggcacactg ttgccggtac aggagttttg gcgcattgac ggttcgtact
     6421 atatgtataa actcggtgag aattttctac attttcttac tgaaaattat ggtgaagaaa
     6481 aagttctcca actcatggag aatatttgga aatttgatga gttccaaaaa gtcgtaacac
     6541 atacggtcgg aaaaaattac gagaccttaa gtcaggaatg gctctactgg ctcaagaaga
     6601 agtattatcc cacgttatcc caaagcgaca tgccttttgc gatttcgcaa accattacca
     6661 aagtaggatt cagttcgaag ccggcctatc tttttgacgg cactaatgaa gaaatctttt
     6721 ttgtggccaa tcgtgacggt tataccaata tataccgtca gaatcttaag gaagccaagg
     6781 caaaagtttt catcgaaggc gaaagaacgc atgaattcga ggcgtttcat ttctttaaaa
     6841 gtaaaatcca tgtcaatgaa caagggaaga tagcttttgc aactaaaagc ggtgagcgcg
     6901 acgtactgta tgtttataac acacaaaccg gtagaaaaga gcatcaatac acctataaag
     6961 atttgaccgg aattatttca ccgcattggg cgcccgatgg caagtccatt gtcttttccg
     7021 ggctccatat gtcgggtaat agcgacttgt ataccgttca tgtaagtacg gatgaaaccg
     7081 acggtatcct gacacgcatc accaatgatt tttatgatga tcgtgatccg gcatggagcc
     7141 ctgacggtaa aactatcgtt tttagttctg atcgcgcggg tttttctccg gaaggaacat
     7201 ataatttatt tatctatgat ctggaaaccg cgcggacatt tcagctaacc agcggcaaat
     7261 atcaggacgc ttcaccggca tggtcaccta aaggtgatta catcgttttt acaagtaacc
     7321 gccagggaag cccgactaat atttcagttg ttccgtttat atatcaagac caatcattta
     7381 tagaaacatc aacgcggggc gcattacaag atagtattta cacgcactat gcaccgcgtc
     7441 agttgaccga tctcacctcg gcggtttttg atccgatttg gactaaagac ggtgatcttg
     7501 taatgaccgc atttgaggaa tttagtttca aattgcgacg tatggaaaaa gcgtatgaca
     7561 aagcgcgtca gttggttccg accgaacgaa aaaaaataga aggtgtgcgt tatccgtgga
     7621 cgacaccgaa agtttcgatc atcggaagta atacccgctc gtatgaaaag aaatacagcc
     7681 tcgatttcgt ccaaggcgct tttcagatcg caccgacgta cggctatggt ggcggcgcca
     7741 caataggata tactgacatg ctaggcgatg atcaatatta ccttaccgtt tttaatacag
     7801 gccaaaccac atccgatatt ttaacagatt ggaattttat tctgaccaaa gttaatctag
     7861 ggcaacgtgt aaaatatgct tatggaggtt atcgttttaa aggcgagttt actcaaatcg
     7921 gcgatcaagg tttacctgaa accgtacatg aggagcgcta tggcggcttt tttcaggttg
     7981 cgtatccatt atccaaattt gaacggatcg aaacgggtat ttcagttagt cgttatcaac
     8041 gcggcaatgc gtttggtgca ctgttgccgc tggatggggt aagcatcggg aataatgtcg
     8101 gatatgttta cgacaattca ctatggggga tcaccggacc gatagatggg cgccgcgcca
     8161 tgctatcgct gggttacacg tcggacgcgt ataaatcgca acaaaatttc tacacgatca
     8221 tcgccgatta tcgtcaatat tttcgtatta caacgcgatc ggcttacgca tttcgaacga
     8281 tgacgcagtg gaatgacggg aaaaacccta gtaatttcgt cgtcggaggt agttgggatt
     8341 tacgtggttt cccacgatgg aatatgccgg gaacgcgttt ttttgtgatg aatcatgaat
     8401 tgcgatttcc tttgatggac gctattcaac taaggtttcc gtttgtcggc ctaggtttcc
     8461 gttcaatacg aggagctatc tttacagaca tcggaaacgc ctggggcgta caaaagaccg
     8521 atggtcgagt gatactgggt catcaaaaat ttgacggttt tttgggtagt gtaggtacgg
     8581 ggttccgtat taattttcta ggtgttatgg ttctgcgttt tgattttgga aaaatgtttg
     8641 atgcgcgcgg ttataagttc ggtaaaattc gtttgcccgg taatcgcgat cgatttgtcg
     8701 aaaccaagtt gaataccaac aaacgatcgg aaggttttgg cgggatcgac attgacggaa
     8761 atataattga aaaaagacgt tggtcacgcg gtatattttt ccaattttgg tttggagctg
     8821 atttctaatg aaaacaatcc ttgtatgtat catcctgatc tttcaattga tggcttgcgg
     8881 cactaccggc aatattcgca ttgcggctta tttcaaaaaa tccacgactt cgccatggag
     8941 taatatcggc ggaaatccag gtcgaaccgg atatgctata aatgaattaa ctttaccaat
     9001 gcagaaagtt tggactaaaa aattatctac ttctctcggc aaggccattg tcgtatccga
     9061 cagtatcatt tatacgacaa cgcatgatgg tcgtttttat gcgattcatt caggttcctc
     9121 acaaacagtc ggttacatta agtttactca tgcatcacaa gccggtgtgg cgatatccgg
     9181 tcagaaaaca gccatcggca tttctaacgg gaaacaaaat ttcatcacac accaagtttt
     9241 tgacggaaaa tttttaacca tcaaaaatac cggtgccatc gaaacaaatc ctttgatata
     9301 tgacgatctg gcgtatgccg gtacactgag caatacagcc gtttgcgtgg atatgaataa
     9361 cggggaaacc gtttggagct acacaacatc tgcaccggtt cacgcttcac ctacaatctc
     9421 gggttcggca gtatattttg gggatgacga cggaatactt tttgcattta atcgcttcaa
     9481 tggcaagttg ctttggaaac tcaagctcga aggaccgatt ctttctgcgc cggtcgcaga
     9541 tgatagcctg gtgtacgttg catctaacga tagtacattg tacgcaatcc atcaaaagga
     9601 agccgtgata gcttggcact accgcatagg atatgataaa cccggtaatt tttatgcttc
     9661 acctgcggtg gatgaaaaac ttttatatat cggcgctacc gacggacgtc tgtacgcgtt
     9721 tgaaaaacgg agcggtaatt tagtatggat gtatgaaact aaaggtccca tttccatcgc
     9781 accggtaatg agcaaatcga ttgtttttat cggatcgcaa gataaaagca tatacgccct
     9841 caataaatac actggagtct tggagtggca gtttgaaaca gaaggacgaa ttaaatctga
     9901 aatggcgatt tacggcggtt cactttttgt cggttctgaa aataacatgc tttataaatt
     9961 tagcgggaaa ctcagatgaa taggcactta cagagtttgt tgtatctttt tactttaggg
    10021 attctcgttc ccgctatgac ttattgtcag aacaaaactc cttacggtta cttaaaaata
    10081 atttctcatg aaaacccggc cttcatttat ttagacaaca ttgcatacga atgggaaaac
    10141 ggtaaagtat tcaagcttct tgtcggacga cacaccctcg cggctcggcg agataatgga
    10201 cttcaaacgg aacaattgga ctttttgcaa aacattacca tcaacagaga tgatacgttg
    10261 tctgtatatt tgcgtttcga aaaagccata acgattcaaa caaaaacgcc caactcagca
    10321 tctgttttta tcaacgattc gctatacggt aaaacaccgt atttatttat acctgaaaca
    10381 gggaataaat acaatgttcg catcagcaaa acaggacatt gggatacgac atttaccata
    10441 gtttcaagcg atacgccatt aatgatggga ttaagaccga aaaaccatga gatgaaatcg
    10501 ttaaacgata atgaatttgt taacgaacga tggcgcaaac aaggtattca tagttataag
    10561 aagcacctgc tcatagcggg tctaagcttg attgtcactg ggtactatac agcggccaat
    10621 aaaaaaacgg cggataacgc ttttgaaaaa gcgaagttag ccaggcgatc ggggaatatt
    10681 gcgttatcaa aaaaattgac agataaaacc cgccgttatg atgatcgcgc ccgaatcgga
    10741 tttatcggct tacaaatcag ttcagcagga tttgccacgc tgcttttact aagtcgctaa
    10801 tcatataaaa aaacattttt taaatacggt gtgacacgct atattcagtc agtatatcat
    10861 tgatttagat aaactgacgc ttcacccgtt catgaaaaaa tttaatatac cgctttttta
    10921 ccgcagctct attatcacgg caataaaaaa ttaccggcgc acgctagacc aacgaaaaaa
    10981 agatttttct cctacacgtc ttgatttcgg tccggtagag tttcttattg cacgccattt
    11041 tggtttttgt tacggcgtag aaaacgccat agaaatttca tacaaagcca ttgaagaaaa
    11101 ccccgggaaa cgtatttttt tgctcagtga aatgatacac aatcccgagg taaattcgga
    11161 tctgcaaagc cggggagttc gatttatcat gcacacgcac ggagagcaga tcattccgtg
    11221 ggcggatatc aatagaaatg acgttgtttt agtgcccgcg tttggtacga ccattgaaat
    11281 agaaaacaaa ctcaaagata ttggaattca atcgtatacc tacgatacga catgtccatt
    11341 tgtcgagaaa gtatggaaca aagcagctca aatcggcaaa gaaagttata ctgtcgttgt
    11401 tcacggaaag ccttcacatg aggaaacacg ggcgactttt tctcatagca ggcaaggagc
    11461 gcctacggtt gttattcgcg atatgaaaga agccgtatta ctcggacgat ttataatcgg
    11521 tgaattgcca ccggaagcgt tttatcagtc atttgagaat cagtactccg agggatttga
    11581 tccgggtaag gatcttaaaa ggatcggtgt ggtcaatcaa acaacgatgc tggccagcga
    11641 cacccaagcc atcgcagatt atttgcgcaa tcttatgatt gataaatatg acttggacca
    11701 agcatcggta catctgcact ttgccgaaac aggcgataca ttatgttacg cgaccaacga
    11761 caatcaaact tccacgtatg gattgttatc ggaaaaagca gacttagcgg tcgttgcggg
    11821 tggatacaac agttccaata cgtctcacat tgtcgaatta tgtgaagaaa agttaccgac
    11881 tttttttatt tcatcggccg agaagatcat cgactcccaa acgataatgc attgggattt
    11941 tcaccataag atagaaaaaa cttctaaaaa cttcattccc caaaaagaaa aagtcaccat
    12001 actgcttacc agtggagcct cgtgtccgga tacgatcgtc gagggaattt tgcttcgttt
    12061 gttgactttt ttttcaaata cacgcgattt cgatgatgtg atgcgtcgct ttcaatgaag
    12121 gttctcaagt tagcgtaata ccgctaacat attttcatat gtttaatctt ttataaagat
    12181 cccgtgatca aatctgtaat aacatattgc gggcttactt ttttatttgt tttcggcgcg
    12241 gcgatatata gccaaaacat ggtaaaaggt ttgccggtca tcgaacatta tgccccacaa
    12301 acatacggag cgggcgtaga caacttttgt tttgtccaag ataagcgtgg ttttatttat
    12361 gttgccaatg gctccggcgt actggagtat gatggcattc aatggcgatt gatcccgtta
    12421 gaaaatcgtg cggccgcctc atggcttacg atcagtccgg aggggacgat attcgtggcc
    12481 agtctcggtg aatttggttg cttagtgccc gacagttccg gttttttgaa gtaccaatct
    12541 cttgttaatc gtcagactca cccgcagtta aaatttcaaa cattttggga aatgtgcgcc
    12601 tcgtcgcaag cgacgtattt gcgtagttat aggcatttag tgcgatacaa aaacggcaac
    12661 atcaaagtgt ggtctgccga tggagacgga ttcgacatca tgggtatggt gtacgatacg
    12721 atatacactc gtatcaaaca tgaaacaatt gttcgaatca tcggtgacag tcttgtacca
    12781 ttgccgggcg gtgacttctt taaagaaata aaaatcaacg gcttccttcc atatcaaaaa
    12841 aacaaacttc ttatcgcaac ccgtaaagat ggtcttttta tctacgaagc aggccaaatc
    12901 aaatctttga aaacggaagc ggacgactat ttaaaaaaat attttgttta cgatacgcgc
    12961 caattgccgg atggtggtta tgtcatcgcg accttgcaag gcggcgtggt gttattcgat
    13021 gcggatttgc gtgtcaaaca aataattgat gaaacatccg gtttgcctgc caacggtgtt
    13081 aattcggttt ttgtggataa taattttaat ctgtggctag gccacagcta tcacggcatt
    13141 acgcgaatcg agatacatac gcctctcact ttgctcaatg cctcaacggg tatcaatggg
    13201 cgcgtcaaac gcatgcttcg ccataaaggc acgttatatg tgatgaccaa tagcggttta
    13261 tttcgattga ccgaaaaggg gaatgcatac cattttgaat tactgcattc cggtgcgccg
    13321 tattattttg atatgtttgt tacgcaagga cgactgttga tctctaccgg aagcggtatt
    13381 ttagaagtaa aaaacgataa cgttgtgtca cacacttcgg tatatacgta tgcggtgtta
    13441 aatagcaatc gggattctaa catcgtctac gcgagcgaag gcctaggtat gacgatttat
    13501 cgtgttggca agtctgagaa atgggaactt gtaaaaacaa taccttccgt caaagtagcc
    13561 gttcgaacca tggcgtatga cgaatacggt gacctttggc tgggaacgga gtacgaaggt
    13621 ctcgggcgta tgcaaatcaa taaaaaggac catctgccgg atacggtcga attttttgat
    13681 agcaaattcg gattgcctgc cggtgaaatt tcagtttggt ccaccggtgc ggcgccatta
    13741 ttctcaggtc atgacggatt ttataagttt tcccgtcagt atcaccgttt caataaatta
    13801 tccgaaactg aaatggcaat gcatcgagat aatagcgaat attggattgg tttgaaccaa
    13861 ggacggtgga tgatcacgcc aacggcttta caaataatgc ctgaaaaaac catcatcaat
    13921 agattacgcg ctataaaaaa tgccgttgat tttatatatt ggcgcgattc ggacggtaca
    13981 gaatggtacg gcggtgattt tcttatcgtt aaacataaac cgaaaaccca aaccctttta
    14041 cccgaacggc ctacggttgt acttcgccgc gttatgcaag gcttggaagt gattttttca
    14101 gatgatgaaa tcaaaaggaa tcatcagatc gtttttgaat ccgaacccac gcagccaatt
    14161 cgttttgaat ttactgcgcc tgtttttgaa atgcggcctc agtttcagac ccgcattatc
    14221 ggcttgaacg aacaatggga atcctggggt agcgatcccg ttcgtttgta tgaacgtata
    14281 cctgccggca aatatcgttt tgaagtcagg gctcgcgatc actttggacg tatttcggaa
    14341 ataaccggcg ctgactttgt cgtcaatccg ccatggtatc ttgcacgtac gatgctgatg
    14401 ttatttgctt tggttgctat cgccttagtt tggttgttta tccgttatcg ggaacaccgc
    14461 cttaagcaga tgaatcagga acttgaacgc cttgttgaag atcgtacgcg tgcacttaaa
    14521 gctaaagagg cgcagctcat ccagtccgaa aaaatggctg cactcgggca gatggtcggc
    14581 ggagtaatgc acgaaatcaa caacccttta acatttattc agcctaatct tgagtatgtg
    14641 cagcttaatt taaaaaaatt tatcagtgat atcaccgcca acgctgtaac tgaaaatcgt
    14701 caggcggaaa ttcgcgaatt tgagcgcgac accattaacg cacttaaaag ttcggtcaaa
    14761 ggtgccgaac gcatacgcga tattgtatcc agtctaaaga gtttcagcgc ttacggtaaa
    14821 aaccaggtga tggatgttcg tttggatcat caactatcag gtgtggtgga tcttattttt
    14881 aatcaaaata acgatattcg gtttatccgt tcatttaaag atacacgatt tgtgcggatt
    14941 gataccatgg aagtaatgca atgtgtggtc ggcgttctaa ataatgccgt cgccgctgta
    15001 catgatgcac tggctgaaaa aatattattg cacgaaaacg ctgaaatccg catctgtact
    15061 atggacggtc ccgatcaaac ggttattata gagatagaag ataatggtat cggtattcct
    15121 gaaactgtac ttcctaggat atttgagcct ttttttacga cgcgtatgat cggtaaagga
    15181 aaaggactcg gtctctcgga agcctatgcc gtaattaaga aacatcatgg taatatagaa
    15241 gtaacgagta agcctcaaaa aggtacgcgc gtaattatca cgttgcctgc taattgatat
    15301 tatcaaattt attggaacct tgtaatataa tctttcgtac aacattcatt cctaattcgg
    15361 tatttaattt tttgattaat aaacggagat acgcatgcgt ctaatgactt ggattttatt
    15421 gtttatctgt tttgtttgta gtgatagcat agcccaaacg ttaacctttg aggaatatga
    15481 accgccttct actttggttg tgcccgaaaa ttttagatac caatctaaat atcccttcat
    15541 agacgtacac aatcatcagt tcgaaatgcc cacgcaggat ttgaaatacc tgaccaaaca
    15601 aatggacagt cttaatatgg ccgtgatggt caatctgagc gggcgtggtt ttaaaagaga
    15661 acaaaataag gacggttcat tccgtttcgg acttcaagac ggaagttatt tgaagggagc
    15721 tatagaaaac gcccgtaaaa atgttcccgg tcgtttgatg gtctttacca atatcaattt
    15781 tggcggcatt gacgatccgg gctggagtgc agaagccgta cgtcaattgg aagaagatgt
    15841 taaaaacggc gcctgcggtt tgaagatata taaaaacctc ggtatgtcat tcaaagattc
    15901 gagcggcaat atcatagctg tagatgatcc gcgcattgat cctatctgga aaaaagcggg
    15961 ggaattggga ataccggtac ttattcactc cgccgatcca tttcagtttt ggcaaccctg
    16021 ggataaaaat aatgagcgat ggctcgaact caaagagcgt cctgatcgta aacgagattc
    16081 gagcaaggag ccctcatttg agagtattat tgccgcacaa catcgcgttt tcaaaaaaca
    16141 ttcaaaaact atttttatta acgcgcattt aggatggatg ggtaatgacc ttgcaaaact
    16201 cggcaagctc atggattcac ttcccaatat gtacacggag atcggtgctg tattggcaga
    16261 gatcggccgt caaccgcgtc aagcgagaga ctggtttatc aaatatcaag atcgtgtgct
    16321 tttcggtaaa gattcatgga atcctcagga gtattccact tatttcagag ttttagaaac
    16381 aaacgatgaa tactttgatt attatcgtcg tcgtcatgct tattggaaaa tgtatggtct
    16441 cggacttcct gatgatgtct taaaaaaatt atattataaa aacgcgttac gcattatacc
    16501 gggcatagac aaatcgttat ttcctaaata gaagcattga attttataat ttgaacattc
    16561 aaaagcatcg cgataacaac gcgatgcttt tttgtataaa aacggttatt tcttacgttt
    16621 attgaaactt tttattaaaa ccaccgttta tcctcccaac cgacagcttt ccttatagtt
    16681 tgttttcatt accgatcaaa taagtgtctt actgaatcgt ctgaatttgt acataacact
    16741 acaacttata ccaaaggata cggtatgcgc cgcacaatgc ttctattatt tgtatgcatt
    16801 gcaacaatgg ctcacgcaca ggatttacca gaaattgacg gctttataag agagggagag
    16861 tggtcagacg caaccgtttt ttcagatttt catttgattt caccccgatc taccgaaaaa
    16921 tattacgata gcacgatagt ctacataaaa cagtcatccg atgccttgtt ttttgccatt
    16981 cgtttttggc ccaaagggcg cgttatttct caatcgtttg tccgcgatcg cagttcggat
    17041 gaagaaaatg aattttttat ccttttagat ttggaaaaca aacggcaaaa cggatatttt
    17101 tttgctttca attttctcgg taatcagcgt gatatgcgca tgtataatca acgccagatg
    17161 tcgcaagaat gggattggaa ttggcaaaac aaatctacga tttttgaaga cgccacgccg
    17221 gataagcctg gatacattga gacggaagta cggatacctg tggataaact gcaaaataaa
    17281 agcccaaaca ccatcggtat tgatgtgcag ctttttgcct ataaacaaga tggtaccagt
    17341 tatttttatc gatcagccct gaaagtgaac tcctaacact caaagggcct tatcaaatgc
    17401 atatatcgcc ttttgtggaa cgggctaaca taaacatgag cgctatacct tatgccgtag
    17461 gatacacgtt tacggattct tcatacgaag cacgtgttgg cggcgaattt agtgtaagta
    17521 ttgatcgcca tacgcttaaa ggtacatata atacagacgc atcgacatta gaagcggatc
    17581 ctttcgtttt ttccttttac aaacgtaaag tttctctagc cgaaaaacgc ccatttttca
    17641 gtcgtgatct ggatgtgtac cgatcaccga taagtttgct ttatacccgc ggtatagccg
    17701 atatcaacta tggtgccaat tatacatttc gcagtaatac actcaaagcc ggtgcatttt
    17761 atgtagaaga agagccgatc cccggggaca cgacgcataa tataaggcgt tttatggccg
    17821 ttcggcctaa tgtggtaacc aaatttgtca attttggcgg aacctttatc cataacgaac
    17881 aaacgaacgg accgtcggaa acgataataa gcggtgatgc gcgagtggat cttccgtccc
    17941 gttgggtttt tgtgccacag ttggcgacta acagagacgg atatgcttcc aaagcgtact
    18001 tgtattacga atttaaccct gcgggcggtc cgtatgctga tttttcatac acccgatttg
    18061 gaaaaaaatt tgatggaatg acagtaataa atcttttcgg taaaaattac gacgaaatat
    18121 ccaattcggt cggttataat ttcatataca accgttccta ttttcatcag atgaatgcac
    18181 gtgtcgaatt ttacaaagcc gaatcactag atcgtgattt tatgttgcag cgcaatatca
    18241 acggtaactt cttctacaaa gtcaacgatt ggttaagcgt caatcattat gccgaaataa
    18301 gtaatgccga tgaggcccta agcgatacga ccaaaaaaaa tcataatgat tatgcacaag
    18361 accataacgt taaaatttta atagggaata atgccattgt tttaggctat aaagaaggtt
    18421 cgtactatgg gcaaaaatta cgcaaccctt atgcgtcgta cgaatccatg tttttttcca
    18481 gagttggcgt atccgctacg gcgtttcttg tcgaaaatga agcgcgtata tttagcgcaa
    18541 aaataaatta ccgtatcatg cctaagctat ttttaagatc attgtatcaa tggaaaagtg
    18601 aaaaaggcaa atccaataaa tacgataatt ttcaattatg gaacagcatg ctccagtacg
    18661 aattttttgc aggaagtaat ttgtacttag tgctcaatct tcagggtgaa gacttggagt
    18721 atgtcggcag gtattgcaaa ctcgcttacg aatttaattt ctaataacta ttgggacgaa
    18781 cgatgcatgc gggacatgcg caggatccag tcccggcttt gttcccgatg cataaaataa
    18841 ccgatttcta aaatgttacc gtccggagca cggagtccct tcataagcga agttttacct
    18901 atggaagcaa ccggtaaaac aacgccgata tgtttaagcc cgaattgcgc cgcttttgaa
    18961 agccactcgt tgcgaagcca ggcttgatca tcgctgtcaa gtacggtcag gagttcgaaa
    19021 tcagcatgta atgcgacgct gcgacggttt ttgacgtgtt gtaacgccac atcaaatgcc
    19081 gtacgaaacg cgccggtggc tgaaaatttt ttccagacta tctccggtaa catcggtccg
    19141 gaaagccatc gtaccgacat gaataaatca tcatgaaggg caacgatccc atcgtttgaa
    19201 tccggttgtt gcaaaggttg taaaagtttg ataaacggtt taccgcacaa tatcaaaaaa
    19261 actgacaaac taaagcaata cccgatttgg gtgatacgaa aatttttata tcattccttt
    19321 tagttttgaa aatcggcgtg tatattggtt tcagtaactt cacttctttt aaaaatcgaa
    19381 cccgtttcca taacccttta tcacaggaat cttattcatg cgacacctac gccattcgtt
    19441 gatgaccgca ctgagtattc tcataatatg ttctgattac acaacagctc aaaattttga
    19501 aaaagatgtt gcggcagcct ttcgatggcg gatgatcgga ccctttcgtg gcggacgaac
    19561 tgtcggcgcc gtcggcgtgc gtcaacaacc gaatgtgttt tacatcggtg ttaataatgg
    19621 cggcgtgtgg aaaacggaag attttggccg aacatggttc ccgatatttg acgatcaacc
    19681 gaccggttcc ataggcgata tcgtcgtggc gccttcggat cctaatattt tatatgtggg
    19741 cagcggcgaa ggtttgcaac gtcccgattt atcggtcggt gacggcgttt ataaatcaac
    19801 ggatgccggt aaaacatgga aacataccgg attgcgtgat ggtcaacaaa tcggcgggtt
    19861 ggcgatagat cccgagaatc ctgatcgcgt ttttgccgcc gtcctaggcc atccgtacgg
    19921 cgcaaatacg gaacgcggtg tttttagaac gatcaacggt ggctcgtcat gggaaaaagt
    19981 tttatttatt gatgaaaata ccggcgctat tcaggtaact ctggacccca aggacactaa
    20041 aaccgtctat gctacacttt gggctgcccg gcaaggtcca tgggaaaacg gttcctggca
    20101 agggcctgca agcggtctgt acaaatcgac cgatggcggt acaacttgga agaagctcac
    20161 gcaaggactg cctaccacca aagaaggact cgggcgtatc ggtttttgta tcgcaccgag
    20221 tttatcatcc cgcttatacg caaccgttga ttccccggaa aaaggcggat tataccgatc
    20281 tgatgatggc ggtaattcat ggcgtttgat gaacccggat aatcgtcttt ggggacgcgg
    20341 tggagacttt gccgaggtca aagtagatcc gcgaaatccg gatatcgtat actctgcgaa
    20401 cgtagcgttt tggaaatcca ccgacggggg caaaaactgg acgtgtgtac gaggagcacc
    20461 gggcggggat gattatcacc gaatatggat caatcccgat aacccggata ttatgctttt
    20521 ggccgccgat caaggcgcga tcattactgt taatggagga aaaacttatt catcgtggta
    20581 caatcagccg acggctcagt tttatcatgt gtcaacggat aacgcatttc catacaacgt
    20641 tctcgggggt caacaggaaa gcggatccgc cagcgtaagt tcacgcggtc atggcggaca
    20701 aataacgtac cgcgattggt tgcccgtcgg cgtcgaagag tggggatatg tagccgctga
    20761 tcctcttaac ccggacttgg tgtatggcgg aaaattaaca aaatacgatc gtaaaaccgg
    20821 tcatacccaa tccgtcggtc cttttccgac aggccattat caacccaatc gcagcccgta
    20881 tcgttttttg agaacggctc cggttatttt ttctcaagcg gatcctacgg ctttatatta
    20941 tgcaggtaat gtattattca aatcacagga tggcggacac gcttggcgga ttatcagtcc
    21001 cgatctttcg cgtgagtcat gggatgttcc aaaaagcatt ggtgtatata ctactgaatc
    21061 tatgaaaacc atggcgcgtc gcggtgtgat ctatactgtc gcaccttcgc ctttggactc
    21121 atcggtgata tgggccggta cggacgacgg tttaatccat ataaccaggg atggcggtaa
    21181 aacatggcaa aacgtaacgc cagctgaaat tacctcatgg agcaagattt cacttataga
    21241 tgcgggtcat tttgatgtca atacagccta cgttgcggtc aatcgcattc gtttggatga
    21301 tatgaagccg tacatttata aaacgcacga cggtggaaaa acatggatca aaatagtcga
    21361 aggattgccc gatgacccaa tcaacgccgt tcgcgaagat catatcaaag aaggtttgct
    21421 tatatgcggg tctgaacggg cagtctatgc ttctgtcaat gatggccaaa attggcttag
    21481 ccttagaaat aacatgcctg ccacttccat tcgtgatatt gtaatcaaag ataacgacat
    21541 cgttgcaggt acacacgggc gttcattttg gattatggat gacatcatgc cgcttcggca
    21601 catcaccgaa gtatcacgtc aagcaccggt atggcttttc aaaccatcta acgcgtaccg
    21661 tgttcgttgg aatctgtata ccgatacacc gcttccgcaa gaggagcccg ccggtgaaaa
    21721 tcctccggat ggagcaatca tctattatca tctaaagcag gatgcgcaaa ccccgatagt
    21781 catcgaaatt aaaaattcgc gtggtgaagt tgtacgacgt ttttcgagca atgattcgat
    21841 ccctatgtca caggataata atttcccatc atactggctt gcaccaccgc agcgtgtttc
    21901 cgccaaagcc ggatcacatc gcctgatatg ggatcttcgt cataatccat tgccgacaga
    21961 gccggaattt tccattgccg caatttatgg taagactaca cctgcgccga catcgccatt
    22021 ggtcatgccc ggtttttatt ccgtagtcat ttcagttaac gggttaacca tgggtgaggg
    22081 cttaaccctc gtcatggatc cgcgtgtacg gatgacggat acggaaatca aagatcaatt
    22141 tgatctgtca atgcggtgtt acgaaataag aaaaaaagga atgcttgtta ctcaacacat
    22201 tcagtcgtgg cgtgtccaag ctgatatata tatggggcag ataaaaagta attcgagtga
    22261 aattaaaaaa tttttaggtt acttggatgc tatcgaaaaa ggtaacaccg ctaccggcga
    22321 caaagggttg caaactattt tgcaatcagc aaccaatatg tttgacgtga tacaacatgc
    22381 cgatatgcca ccaaccgcag caatgtacaa taccgttcgc gaaacggaaa agagttggga
    22441 aaaattaacg gctgaatggg aaaaactaaa aacggtttac ctcaacgact taaatgctca
    22501 tttgaaaaaa aataaattac ctgaaatatc aaacccataa tagcaatctg attatttttc
    22561 attattcatt taaaatcgga gaaatatcat gaatttacgc gcttggttaa tgctatgttc
    22621 gttgctgatt tcgacgcacg tcgtagcgca aaagaaaaat gctgaaacta aagaccccat
    22681 gccttcttca acctttggag gactcaagtt ccgtagtgtc ggcccggctc tgacaagcgg
    22741 ccgtgtcgta gactttgccg tcaatcctaa aaatattttc gaatattacg tagctgtcgc
    22801 atccggcggt gtgtggaaaa caaccaatgc cggtgtgacg tatgaacctg tttttgacgg
    22861 cgaagggtca tactccatag gctgtgtggc gattgatcct tccaatccta atgtggtatg
    22921 ggttggcagc ggtgaaaata acaaccaaag agcggtggct tacggcgatg gtatatacaa
    22981 atctgtggac gggggaaagt cctggaccaa tatgggtctt aaaaattcag aacatattgg
    23041 ccgtatcgcc attgatccca ataattcaga catcgtttac gtagcggcat atggcccggt
    23101 ttggagtgcc ggcggtgacc gcggtattta caaaacgacg gacggcggta aaacatggaa
    23161 agccgttctc acggtcagcg aaaacacggg gttcaatgaa gtgatcatgg atccacgtaa
    23221 ttccaatgta ctctatgcgg cagcccatca acgccgcaga tccgttttca cctacatcgg
    23281 cggcggccca gagtctgcgt tgtacaaatc tactgacggc ggtgccaact ggcggaaaat
    23341 caattccggt ttgcccgcgg tagatctcgg tcgtatcgga atcgccattt caccgattaa
    23401 tcccgatgtg ttgtatgcga tcgttgaagc cgctgaagga aaaagcggtg tcttccgttc
    23461 cgataatcgt ggtgaaagct gggaaaaacg aggaaataat tcctcatccg gaaattatta
    23521 tcaggaaatt ttttgcgatc cacaaaatat agacaaagtt tattatgtgg atttatttgt
    23581 aatgctttct ttggacggag gaaaatcttt taatccgatc ggcgaaaaat acaaacacgt
    23641 ggacaatcac gcgctttgga tcaatccggc caatacaggc catatgcttg tcggttgcga
    23701 cggcggcata tacgaaacat atgataacgg tgcaaattgg gaatataaat ggaatcttcc
    23761 tgtaactcag ttttacaaag tggccaccga caacgccaca ccgttttatt atatctacgg
    23821 cggcacgcaa gacaataaca gtatcggcgg gccttcgcgc acactaagcg ccaatggcat
    23881 catcaacacg gattggttta tcaccaatga aggcgacggt tttgaatcgc agatagatcc
    23941 ttttgatgcc aatatcgtgt acgcacaatc gcagtacggc ggacttgtgc gctacgataa
    24001 aaagagcggg gaagtcattg acatcaaacc ggttgaagga gaaaacgaat cgggattgcg
    24061 ttggaattgg gatgctccgc tgcttgtttc ccgtcacaag gccaatcgtt tgtatttcgg
    24121 agccaatatc cttttccgaa gtgatgaccg tggtcattca tggaaagcga ttagcaaaga
    24181 catgagtcgc ggtttggatc gtaataagtt atccgtgatg gggcgtgttt ggagtattga
    24241 cgccgtagct aaaaacggct caacggatat ttacggtcag ctcgtaagta tcgccgaatc
    24301 gcctttggac gagaatgttc tttataccgg cacagatgat gggcttatcc atgtatcgcg
    24361 tgacggcggc gcatcttgga ctgtcgtgga taatattccc ggagttcctg aacgcacata
    24421 tgtcaatcaa atcatcgcaa gccaacatga taaaggaacg gtatacgcga ttttcaatca
    24481 tcatcgttac ggtgatttca aaccgtatat cttccgtagc cgtgattttg gaaaaacgtg
    24541 gtcagccatt caaaacaact tgccggaacg cggatcagtt tattgcgtag gtgaggatca
    24601 tatgcgcgct gatttgttat tcgtaggaac ggaatttggt gtgtttttta cgattgatgg
    24661 cggtcagaaa tgggtgcaac tcaaatcagg gctaccgaca atagctgttc gcgatcttga
    24721 aattcaacgt cgcgaaaatg atcttgtcct cggtacattc ggtcgtgggt tctatgtact
    24781 cgatgattac acgccccttc gtcatctcaa aaaagaagac ctttccaaag aagcaatgct
    24841 ttatcctgta aaagatgcgt tgatgtttat cgaatcacgc ccgttgggac ttagcgaaaa
    24901 atcatacatg ggtgaaaatc tatttagtac ccctaatccg acagtcggcg ctgtgtttac
    24961 gtattatctg gccgaggaca tcaaaacggt caaagaaaaa cgccgcgctt cagaaaaaga
    25021 gaaggcttcc aaaagtgaat cggtatttta tccgtctatg gattcactca aaacggaaga
    25081 actgcaaact gaaccgacat taatttttac gatcaaagac gatgccggac gtatagtacg
    25141 acgcctcaaa gcgccggcca aaaaaggtat gaatcgtatc acttgggatt tccgttatcc
    25201 atcgccgggc ccggttactt ttaatgtccc ggtttcatat aatttctatg acgtgcttga
    25261 aaaaggccac gtcgccatgc ctggaaatta tagcgtcacg atggggaaaa tcgaagatgg
    25321 aaaatatacg gaattaactc cggcgcaatc ttttaaagct gtagcattga atgcatccgc
    25381 attgtctgcc acggataaaa acgcattcga ttcgtttgcg aaatctgtct cggattggcg
    25441 ccgtcaaatc agttccgccg atgcgtatcg cggtgaattg gcaggtaaaa tcaagtttat
    25501 gaaagccgct gcattggatg ccgtggaacc cggatcagaa atcattgaag ccgtttacac
    25561 tgttgaacgc cgcctaaagg aatttaacct caaactcaat ggtgataata ttcgtgacaa
    25621 atatcagttt gaacgcgctc cgtccgtcag cgatcgtatc ggtcagattg agtttagcat
    25681 atggaatgcg acagcggcac cgactgaaac tcaaaaacaa tctttggctg tagtagccaa
    25741 acaaacaccg gcgcttatgg atgaattgaa atccatagac accgagatca aacgtatcga
    25801 atccatgctt gagcggggta aggcgcctta tactccagga cgtatgccgg agaaaaaata
    25861 aaagataatt tttagttttt ttatagtcta cagactgcct gacgaatata aaattctcag
    25921 gcagtttttt tttgtttgat tcaggctttg atttttagta cccttttttg gttttctaat
    25981 cccgtctact aaatagtatc ttcatgatgc ccctcaggat ttcaatattt tctttatact
    26041 tttttatttt gcacgcactt tatgcacaga atgaagaagg attaattcct acccaatatt
    26101 attcaaccag tcaatttttt gcgcataatc aaaattgggc cgtgacacaa gatcaacaaa
    26161 ccggattgat ctacgcggga aataatgttg gcattttgga gtttgacgga gccacgtggc
    26221 gcatcattca cacggcgaac cgaacgatgg tacgttcgct ggcttatgat ccggtcggtc
    26281 atcgtgtttt agtgggagca cagggcgaat tcggttatct caagccagat tcagttggta
    26341 ttatgcgtta cgaatcattg tctgccgtct tgccggaaga agcgcgtcgt tttgtcaacg
    26401 tatggcgcat ttgggttaca aatagaggta ctttctttca aaccaatacg gaagttttca
    26461 tacttcaaaa cgaaacattg cgaatcataa aaccggatca gtcttcattt catttttctt
    26521 ttttggctaa cggcaatgta tatgtgactt tgcgcaatgt aggattacaa aagtttaatg
    26581 gtgataaatt cgaaaccgtt ccaggtggag atcgttttca taaaattcct atttatgctg
    26641 tgctatcgta tgcgcctgac acggtattga tagcaacaca agaaggattg tttcttggat
    26701 taaataacgg agtgattccg tttggcgaaa ccagcaaacc gtttctcaaa acaaaccgcg
    26761 tctacaatgg tttgaacttg cccgatggac gtttcgcact ggctaccttg acgggcggtg
    26821 tagcgatttt aaatcgtaac ggtacaatcg cacgcgtact ggacaaaagt aacgggctcg
    26881 gtgacaatgc agcaaaaagc atatttcttg accgtgacaa agctctttgg gtttcattgg
    26941 aaaaggggat ttgccgaata gaatattttt caccgttcac ttatttcgat gatcgcgtcg
    27001 gattattaag tcagtttgaa actttcgtac ggtggaaggg gaaattttat ataggcacta
    27061 aggacgcatt gtatgtgttg gatcgccagc caagttcaca tatcccatac catttcaaaa
    27121 cattatacga tgtacccgag gagcattggt ccttactgcc gatgggtgat gttatgttgg
    27181 atgcgacatt ccgaaatggt atttatgaaa tttcatcaca cggcagaaaa cgtatttcac
    27241 ccaatagtac ggcgttttta ctgcgatcac gattggatac gcatcgtgtt tatgccgcac
    27301 tctgggatgg tttgtcgtcg gtatatttgc gtaatggaat ctgggtggat gaaggtcgtt
    27361 atgcgggtat taccgacgaa gtgcgcgtca tcgccgaaga cagcgacggt tcattatgga
    27421 tggatcaaaa aaccggcggt tgcgcacgta tgtcccgcat caacgatagt atatacgtgc
    27481 ggcggttttc agtcaaagac ggtttgccta acttgactat taatccttat tttttctttg
    27541 gacgaatgtg gttcgctacc gataatggcg cactctacag atatgatgaa aaagcatctc
    27601 gattttatcc tgatacaacg ttgcatcatc ggttaggaat cgcaacatct aaaatgattt
    27661 ctccgattgg ggaaacatcc gatgggacat tgtggtttgc tttgggtaat gacgaatcaa
    27721 tgcgttttga acctgcctat gccgtgcctg acggcgataa ataccgtgtt acaattttgg
    27781 cacatggacg attaaagatt aaactttcgc cgggtaattt ctttgttgaa gaaaatgccg
    27841 tctggtttta tggacatgac aatttttatc gtgtagatcg tttacgcgcc aacggacttt
    27901 cacatgattt caaagtaaat atccgtaaat ttactctcaa cgattcgtta ttatatggcg
    27961 gcgctcttca tagcgattta cctacgatcg tcgtaccgtt cggaacgtcc gtcattcgga
    28021 ttgattatgc agccaccgcg tttgaaaatc aaaatgatat acatttcaaa tttaaacttg
    28081 atgggtttga cgaagaatgg tctgcacgca ctacagaagc cttcaaagaa tacacacatt
    28141 tgcctgaagg caaatacaca tttcgtgtca aaggttacaa tgtctatgaa tttgaatcgt
    28201 cggaagcgtc catagatttt attgttttac cgccatggta taggacatgg tggagttttg
    28261 ttctgtattt ttttgtcgga gcagggatag tgtacgctgg tttccgttgg cgtgtgcgtt
    28321 tgttgcgttt gcgaaataaa gagttattcg tcaaagtaga agagagaaca cgcgaactgg
    28381 cttcagctaa tcacgagatt gagacgcaac gcgactttct taaaaacatg aaccagcaac
    28441 ttgaaagcgc actcaaagac cttcgcgaaa cgcaaacaca actcatccaa actgaaaaaa
    28501 tggctaccgt cgggcaaatg gttgcgggcg taatgcacga aattaataat ccgctgactt
    28561 ttattttgcc caatatcgaa tacatcgaaa aaagctggaa aaaattagta acatataccg
    28621 aacgttccaa accgcatgtc gcaggagcac aagatccgga actgttagct attatccaag
    28681 acatcgaacg cggtcttctt attgatgaaa ccttcggagc actagattcc atgcggcgcg
    28741 gtagtcagcg tatcaaacag gtggtcgtca atttgcgtgc tttcgcggct atggactact
    28801 tgcccaaatc agaaaccgtt gtggatgccc atatcgagca gaccgttgat ttgttctttg
    28861 ggactgtaaa ggatttatca tttgttaaaa agctttctta tccgacacca attaaggtgc
    28921 gcattcagga gttcaatcaa tccgtcgttg cgttactaaa taatgctgta caagccatcc
    28981 gtgatgcaga aatgcagggt acgctcgaac ggggaaaagg tgttatcgaa attcgaaccc
    29041 aatcacataa gggcgatgct aacacccttg agtttagtat ctctgataat ggtatcggca
    29101 ttccggaaga tattcttccg agaatttttg aaccattttt tacaacacgg tccgtcggtc
    29161 agggcagagg actcgggctt tcggaagtat atgctgttgt ccataagcat aacggcctta
    29221 ttactacaca aagtaaagtc ggacatggta caactcttac catcactctg ccggcttgat
    29281 gtcaaataaa aaactgcgtc ggaaaatatt ccaacgcagt cataatctta aaagtaaaag
    29341 cggtatttat taattcttaa agcactattt actgtcttgt aatcttttaa gtgtatcacc
    29401 gatacgtctt ttattagcat cgtctttgac taatttttgt gctttagtaa aactttctat
    29461 agcccccttt ttgtcgccta tagcctcaag cccgtcacca agactgtcgt atacattcca
    29521 tgattctgga aattttttag cattagcacg gaaaacttca accgcttcgg tggctttgcc
    29581 ttgaccaagc aattgatacc cgaatgtgtt gaggtctgct tctgtggcgt tatctatagc
    29641 tcttttctta acagcttcag cttctgtttt tttaccttgt ttgtccaaaa taattgattt
    29701 cagaaaaaaa gcacttgtgc caccgccgct attgatggcg cgatcagtcc atgacaaggc
    29761 ttcatcaagt gaattattgc gcagcgcaaa ctgtgcggcc tgtgataacg aaccatgatc
    29821 ccacgcttcg cgatttttga gacgttggcg aatatagttg agtacgatct gaggtgtatc
    29881 aaagctgatt gtaaacggca ctttgatttt ttcccagcgc agataggcga tggttttatt
    29941 gcgatcggta ttatcgaatc cgtaactgag acgatcttga gaatccgcca tcatctgcgg
    30001 tttgacggta acacgcagtg cgtcttcaga ttcttcataa aagaaactac cccatgccgt
    30061 atagttattg ttaaaaatga ttacccattg atcggcttct gggatcatgt gtatgctata
    30121 cgtaccggcc ttgagagtct gaccttctat tttagcgtca tgtgtcagag tgatcgtcgt
    30181 attttcgtta gcaccggcac gccagggcat aggttttcca tgtccgaatg gattgctttt
    30241 ttccatccca tagggtacta atttacccca gatttcacga ccattgaccg caggactact
    30301 gtaggaaatc gtaactttgg aaatgccaac ctcttgcatg atcattgcct caggactggc
    30361 atctggaaat cgcaaatcct gcgctgaagt catcataccg caagccaaga tcacaaaaca
    30421 taacaacgtt ttcatggttt ctcctaaaag gattaattaa cagatatggg acaacgaaaa
    30481 gtatattaat aacgtataga tgatgatttt gttccgcgat attttcagat ttgatactta
    30541 taaaaaaaac tgtttatcat ggttttttaa tacctcatat tgtctgtgta accaatatat
    30601 ccattatctt tagtttttat gattcttatt gccggtgtca gcatagccgt atttatagcc
    30661 gctttattga tttctaaaaa gcaaaaatca caaggcgaca ttattctcac ggcatggatg
    30721 atgattatcg cagtgcaaat gctgttttat cacgcgtata taaccgacat tattttcagg
    30781 tatcctatct ttcttgcgtg ggaaagaccg cttccgttgt tacatggtgt attattgtat
    30841 tggtacgttg cgacgatgac cgatcaaagg ccatcctcta agtttctttt gtatcttcat
    30901 tttattccaa ctttgttttt ttatctggta cttattccat tttttttgcg accggttgaa
    30961 gaaaaaattt gggtctatcg taacgaaggc gccggatatg aaatattgga tgcaattcgg
    31021 atgggggcga ttatcttatc gggcaccatt tacatcatct ggtcagctat tcttttgtcg
    31081 cggcacaaac ggagcatttt aaaccgtttt tcatatgaag aaaaaatcaa tcttcagtgg
    31141 ttacgcattc ttacatgggg cctaggctca gtttggctgc tgatttttat atccgatgaa
    31201 gtgttatccg ttggcgttac aatttttgtg tttatgatcg gtttttttgg tattaggcaa
    31261 tcacgcattt ttaaaacaac tgaaaataat ttagaagtac ccgaaaaaga aaaatacgcc
    31321 aaatccggct tgaccaaaga aacagctgag accctatacg caggcctgat gagactgatg
    31381 aatgaagaag ccgtatatca gaaaaatgat ctctccatat atgaccttgc aacgcggctt
    31441 aatgttcacc ctaattacct ttctcaggtt atcaacgaac ttgaaagaaa aaatttttat
    31501 gatttcgtca accaatatcg cttaaatcat tttatcaaac tgatatccga cacacgaaac
    31561 cgttatttga cgtttcttgc tctagcctat gaaagcggtt tcaattctaa atcttcattc
    31621 aatcgatatt tcaaaaaggc gaccggcaaa acgccgtcag agtatttttc gagcgaagga
    31681 ctagtgaatt aactctgaat cagaaaacga atttaacaaa tattcaccaa acatcgaatg
    31741 acctcttttc ttctttacat catattcgtt tatctcgtcg ggggtctgtg tggggcgtat
    31801 tatctactac actggaaaac aggcgaaaac ctccgcgatc acgggagtgg aaacctgggt
    31861 gcaacgaacg ccggaagagt gcttggcaaa acaggcttta ttattacttt tttatgggat
    31921 gccggtaagg gctcagcggt catttacggg gctcgaattt tggattatga tcaacacctt
    31981 atatggattt gtgccatcgc cgtagtgacc ggccatattt ggccgatcca attgcaattt
    32041 aaaggaggca aaggtgctgc tacactgatt ggaatattag tagcactgga cgcatatttg
    32101 ctgtggccat tactttttat ttttctggca tcttttgtga ttctacgaag attcggtata
    32161 gccggtgctc tggccttctt tactttacca ctcgtcgtat ggctgcgagg ctatgaacct
    32221 ctctctgtaa tactcagtat cattctgacg atcattgtca ttttcgcata ccgcgatcat
    32281 atcgttactt ttgtaagtgc ctttaaaaaa agaagccgga ataaataagc cgctatctat
    32341 attataaaat aagttgctca aattttagga gaagctatgt ccatacgtcc cgtcaaacgc
    32401 attattcagt ctacacctac gatggaaggt gccggtgtaa ggctacgacg cgctttcggt
    32461 tttggtaata cggaggatta tgatcctttt ttgatgttag acgatttcag aaacgataat
    32521 cctaatgatt atatggcagg ttttccgtgg catccgcatc gtggtataga aaccattact
    32581 tatgtattag ccggtaatgt agatcacaaa gacagtttag gcaatgcggg ttcgcttggc
    32641 gcaggtgatg tgcagtggat gacagccggc agcggcattt tacatcagga aatgccccaa
    32701 ggcgatgcac aagggcgaat gcacggtttt cagttgtggg ccaatcttcc ttcttcgctc
    32761 aaaatgacag gtccgcgata ccaagatgtc aaaagtaaag acatcccgga gatttcagaa
    32821 gatgaaggta caaaaatacg tgttgtatgc ggcgactacg gaggacagaa aggacctgtg
    32881 gacggtatag ccgcagatcc gcggtatctt gatgtgtttg ttccgtcagg aaaacgtaag
    32941 actttaaaag tagaaactta ccgacaagcg tttgcatata tatttgaggg ctccggttct
    33001 tttcgcgatg cttcacagcc gcacggcatt ttgactgagg cgccggatga ccatgataat
    33061 gtgtcgcgct ccatggccgg aaatcgttca ttggtgcttt ttgactccgg tgacgaagtc
    33121 accgtacaag cgggcgatga aggtattcga tttttattgg tttccgggcg accgatcaaa
    33181 gaacccgtcg cgtggtatgg cccgatcgtg atgaatacca acgccgagct tcaacaagcc
    33241 gtttccgaat tacgaaacgg aacttttatc aaacacggat aatttatttt aaaacattca
    33301 tggctaagcc ggtgtcaaaa cctatttttt tccaacatca gaaaacttac gacgttggtt
    33361 cgaaaaatat cacggcttcg ccgacgaatt agtcgtaggt tattacaaaa aagctacggg
    33421 gaagccaagc attacttggc aggattcagt agatcaggct ttgtgtttcg gatggataga
    33481 cagcattcgg cataagatta acgccgaagc gtttcagaat cgctttacac cacgtaagcc
    33541 gggtagcatc tggagcggaa tacaaagaga tttagagaac tccacaaaaa aagaatggta
    33601 tatccttatg gtttggaagc tttcaaaaac gcgatataaa aaaatctaaa ccatattcct
    33661 acggaagagc aaatgcggat ttaccggatg ccctcatttc gatctttcaa accgatacca
    33721 tagcgtggga ttacttggaa ttgaaatcac cgtcgtatcg caaagcggcg atttggtggg
    33781 tggtaagcgc caaacaggat gccacacaaa aaaagcgtct tgatatcctc atcaaggatt
    33841 cagaaaataa gcgccctatt gcacttttac gccgtccttc aaaaaagtag ttgccacgcg
    33901 ctcaaagcgg ctgtattttg caccaacata tcatattatt tcctaattca ttagtatggc
    33961 gcgcacacct tcgaacatgg cctcgctcgg gacatttgct cctgattttg acctgcctga
    34021 cgttatctct gaaaaaaaat taaaactggc tgattttaaa gaaaaaccgg ctttgcttgt
    34081 tatgtttatc agcaggcatt gcccttttgt agtgcatgtc gaaaaagaat tagcgcgtat
    34141 cggaaaagat tacccgcaga tcggcattgt agccatttgt tccaacgata tcgaaaatta
    34201 tcctgaagac gcgccggatc aattacgtga acaagctaac cggttacaat ttacttttcc
    34261 ttatttgtac gatgcctcgc aagagaccgc taagaattat aaagcagctt gtactccgga
    34321 tttcttttta tatgatcaat cgcgccgttt gatttacagg ggacagttgg acgatagtcg
    34381 ccctaatagc gtcattccgg ttactggaag ggatctacgc aatgcgatag aggccgtgct
    34441 ttccggccat cggccttcaa cagagcagcg accgagcctc ggctgcaata tcaaatggaa
    34501 acccggtaat gaaccctaat gcggtatgca tatccacgta gtggtattac atatttaaag
    34561 taacataaaa cagaaggcgc gatcatgttt gattggttta agaagaagaa aaaactcaac
    34621 ccaaagccca aacaaagtac aaattttgac tccggtacga tgtacgatcc attatcgccg
    34681 acatatgtta ctgcaggttt agcgtcggga gctattgagg aggaaactgt agatgatcga
    34741 aatattgaag actcttcgaa ttcagatgat acaccggaaa gcagttctga cgcatccgat
    34801 agcggtagtg attccggagg gtccgactaa tttttatgag taaatgaaac aaattgactt
    34861 ctgaagcagt atcttatctt ctaatcatcc ttattcaacc ctaaagaagg attacaacta
    34921 tggctgttcg ttctaaaaaa gctgtttcca aaaaaaacgt agcccgtaaa cccgcaaaga
    34981 aaaaacaaat caaaaagaaa gttcatttta ttcccaaagg ttttcacaca ttaacacctt
    35041 acctcagtgt acacgacggt aaagcggcgg ttgagtttta tgaaaaagcg tttggcgcca
    35101 aagaaagaaa aggtcgcatg tccgatgaaa aaggtaaaat catgcatacg gagattaaaa
    35161 tcggcgtatc acactttatg ctttctgatg aattcccgga atacggcaat gtaagcccta
    35221 aaaccgtagg gaagtcgcct gtacagctca tgatttacgt acccaatgtt gacatcctat
    35281 tcgaaaaagc tattgccgca ggagccaccg tagttatggc cgtggaagat cagttttacg
    35341 gtgaccgctc gggacgcctt ttggatccgt tcggttacca atggcatatt gcgacccata
    35401 tggaaaacgt ttctccaaaa gaaatggccc gccgcgccgc aaagctatat ggtgcacagg
    35461 cctcataaag cattttttaa aatatgaaat ataagtcatg cacaacggat ggcgaattgc
    35521 ttcaaattct ggatttgcaa aagcgcaatc ttccggattt gttaacaaat gaggagcgcc
    35581 aaagccaggg atttgttacg gtaaaacatg attttgagac acttgaagct atgaatacgc
    35641 cgtttgcaca tagtttggcc atctccgatg atcgtgttgt gggttatgct ctgacgatgg
    35701 agcgttcgat ggaagcacga atacccatcc ttcaacctat gttcactacg ttggatacat
    35761 tatccatcca aaacagaaaa atgtccgatg aaggatatat cgtcatgggg cagatctgcg
    35821 tagaaaaagc ttttcgtgga aaaggtattt ttcgtgggct ctattcgcat atgcgttcac
    35881 gccttggacc ttattttaga tatctcatca cagagatatc agagcgtaat gtacgctcgc
    35941 ttaatgccca ctccgcagtc ggctttcaaa aactaatcac gtatgaagca cccgatggcg
    36001 aaaggtgggt gatagtcgga tggaattgga atcaatcaaa cacaaagtaa ggatacaagt
    36061 gcatggccga aaacaaaacc aaagctacaa agctgagtgt ccaggatttt ttaaagacga
    36121 ttaaagatga gcagaccgtc aaggattgta atgtactgat caaactaatg aaaaaaataa
    36181 caggcaaacc tgctgttatg tgggggccat caatcatcgg ttttgacagt tatcattaca
    36241 aatatgaaag tggacgtgaa ggtgatatgt gtattactgg tttttcaccc cgcaaaggta
    36301 aaatatccat ttacattaca tcaggttttg aacgttatac tgctcatcta aaaaagatcg
    36361 gtaaatgtaa aacgagtaaa gcatgccttt atgtaaagtc gcttacagat attgataccc
    36421 aagttcttga agaaatgata aaggattcga tcattttcat gaaaaagaag tggaaataag
    36481 agtttaagat aactcaagaa tcaaaaaaac agaatatcac aagctgttat ttattataaa
    36541 attgaccata tcaccctata agatgacacc tgtttcatgt atcatttaac acgctgaagc
    36601 gatcatatct atccttttta ttaaatttta actgtttgtc agtaaaaagt tatcaattaa
    36661 aaagaagggt agaatatgaa acatgtaaaa acagttattc tgttattggt tttctttagc
    36721 tcgtttgttt ttagtcacgg cggcggaaag aatgtaaaac ttcacgtcaa tccaaagtgg
    36781 aaacagtgct cgatccaact tgattcggct ttaaaccagt cagcatggcg ccagtttaca
    36841 cgagaagcag gacttgtatc atattttcga cctttgacgg atgctaagcc gatgggtaaa
    36901 ggaaactatg aattttcttt gcttcagtgg cagtccaagt ttgatgatac ggatgacgca
    36961 tggaatcata cttttgttca tccggattcc acgcactggc ttaaagaaaa tgataggttg
    37021 cctttcccgg gattgacttt tcgattgggt atatcagaaa aaatggactt agcggcttat
    37081 tggacatcat caccaggagc taattatggt ttttttggtg ctcaggtaca atataatata
    37141 gttcaaaatt ggcttaatca gtggtcggcc tcggcacgcc taagttttgt ttccctctat
    37201 gggccaaaag atctcgatct ttcggtatat ggtttagatg tgattgccag ccgtgatttt
    37261 acagtctatc ccaaatggtt gaaagttaca ccttatgtcg gtttatcaag ttatatgaca
    37321 gattctcatg aaaaatcatc aaagttaaac ctgcatgatg aaacagcttg gggtgttcag
    37381 agtatgttcg ggggagttat ttcgatttcg aaggcgagac ttgccgcaga atataacatt
    37441 tcaaatatca gcacattttc cgtaaaacta ggtattgttt tctgaattaa ttgattacta
    37501 ttctccacag taaaacgctt tgttcttata ctaaaaaaag acgaagcgtt tttttattaa
    37561 tacctatcag ctttccgata acaatttgaa aacccgaaat ccaaaagcat tgaattacta
    37621 attaaccgat acagtacgct gtaaattcta actttatagt tttaattatt cggcatataa
    37681 tttgtaatat acaaatacct atacatttgt taaaataatc taagatggga tttatgagaa
    37741 ctctaacatt ggtgataatt tcatgttttg ctttgagctg tgagtcaatc aatcaatcat
    37801 tgttgaacca tgatgatggt tcagaaaaaa actttttatc ctacgaagta accctaataa
    37861 ataatgatgg taattattca agatcaggtg ttcttcggat tcaatggaat aatgaaggat
    37921 caattactgg tcaatggatt attaaccaaa acgaatctta tgaggtcaaa ggacaaaaac
    37981 aagattcagg ttttattatt gagttatttc cttatgggat agattcaggg gaaagcatca
    38041 gcggctcttt ttcaactgat aaacagacta tcagcggcga ttgggagcag atgactatag
    38101 ccggcccttt aactggtagc tttaatggat tctcgacgga cattcatcct tgatgatatg
    38161 gatttggatt taaccaacgc attatataaa catacttaat taaaaaattt ctcttattca
    38221 ttcttttgaa tgactcctgt atagggtgcg accttgttgg cgcacctttt attttttatt
    38281 catatgtaaa agtgattctt ttttatatat aatttttttc gtatttttaa gaaaatccaa
    38341 attgtttaaa tcaatggata ctcccgttgc acttgtcaca ggctctggta aaagattggg
    38401 ccgaaaaata gcattggctt tagctgaaac cggctacgat atcattatca attatcacca
    38461 gtccgttgtc gatgcccgta aaacggaaaa acttatacgt caaatagggc gcaaagcggt
    38521 gttgatcaaa gcggatgttc gacgtccgga tcacataaaa aaaatgttca atgaagtgat
    38581 taagaaattc ggtaaaattg atgttctgat aaataacgct gcgatttttc caaaaaaaac
    38641 cgaacttcca aatatccccg aatctatatg ggatgatgtg attgactcga atcttaaatc
    38701 aagttttttg tgtgcgcaag aagctgcaaa atacatgtta cgccaaaaat ccggtaaaat
    38761 aattaacatc gcatcgttgg gcgcattttt atcgtggaaa aactacatcc catataatgt
    38821 atccaaagcc ggcgtagtta tgctaacgcg cagcttagct aaagcattgg ccccggatat
    38881 cactgtcaat gctgtcgcgc ccggtacgat catagttccg gaagaagagg tattgtctga
    38941 acacttaccg acaatggatc gcattcctat gaaaaaatac ggcaagcccg ccgatataac
    39001 ggccgcagtt ttatttctgc tgcaaaacga atacatcacc ggtcagatcg tcggtgtgga
    39061 tggaggggct acaattccat aatacttgag acgttaaaca aaaaatttaa aaggtaaaat
    39121 tttattgatt tttatttata attctatata tttgcgaccg gattttgcca caatagctca
    39181 gcggtagagc agctgtttcg taaacagcag gtcgcaggtt cgaatcccgc ttgtggctct
    39241 tcaaaacctg atcatcatgt gatcaggttt tcctttttag ctctcacgta tcccgctaat
    39301 tagtttatcc ccaaacacct cgttccaaat cccatttaag gttaattcaa attttcttga
    39361 ataaaccttt atttgcattt atctttacag cacttttcca ttccaaataa tatatatcca
    39421 tttatgaaca caagtgatac cattcagttt tataacgcat tggtaaaaaa tcacgaacgg
    39481 cgcattcaag ccgtttttga aaaacatctg aagcaatgtg caaaggaaaa actgaccttt
    39541 ggcgggcggc atatgtacag tttcctgaga cccaatttta ttacactaga acaataccgt
    39601 tatatccaat acgtttgcaa gatactccgt aatgccgtta cgaaatttaa aaatgccgcg
    39661 ctcgaaaatc caatcatcat ggagcaagcc ggtcttttag atagggaacg cgaactcgta
    39721 atgatggacc cgggatatga caggctttcg atcaccgcac gatgggattc atttatggcc
    39781 ggcgacgaat taaagttcgt ggagctcaat gccgaatgcc ctgcagggat tgcttattcc
    39841 gatattgcag ccaaggtgta tgataagtta ccgatgatac gcgaatttaa aaaacacttt
    39901 aacgttgaaa aatttaccat ccgtcaggaa ttactgaacg gattattatc tacctacgcc
    39961 gtttggcgag gaaacaaaaa gaacaagaaa ccacgcatcg ccattgtaga ctggagagaa
    40021 gtaccgacct ttaccgagtt tcaacttttt caggaatttt ttaaatctaa aaaattagac
    40081 tgtattatcg tagatccgcg tgatctgacg tatgaaaaag gtcgcctcat ggctgggaaa
    40141 aaggaaatag atttggttta taagcggata ttgaccaacg actgtgttga aaaaccggag
    40201 gaaacaaaag ccttagttca agcataccgc gatcaaaacg tatgtatgat caatcctttt
    40261 agggctaaac ttgttcataa aaaatctatg ttcgctgttt tgacccatga aaaaaatcaa
    40321 aaacttttta atggcgaaga actttcagtc gtactcaaac atattccatg gactcgaatg
    40381 ctccgggatg aaataacata ctttaatgga aaacaaattg atcttttgga ctacgtatct
    40441 acatataaaa acaatttcgt cataaagcct aatgatgagt acggaggaaa aggggtttgt
    40501 ttaggaatgg aagctaccga ccaacagtgg agcgccgtgc tccatgaagc aaagaacggg
    40561 gagcactatg tagtgcagga gcttgtgaaa attccaaaac aaccttttcc aactgttatc
    40621 aatggtaatt tagaatttgt tgatatggtt gttgatatgg atccttatgc gttcggtccg
    40681 aatgttgaag gtatattgac acggttatct gcatcttcac tagccaatgt tactgccggt
    40741 ggcggtacaa ctccgacttt tgttattaca aaaaaaacaa aaaaggccaa aaaaacatta
    40801 aaaaatgaga ttagaagatt catatccaaa caaaaaccta aattaagtta tttaaaaaca
    40861 aataataaaa agactaaaaa gaaagttaca gtaaaaaggc gctcaaaaaa aattgaaatg
    40921 attaataaaa acaaatattt aataatatga ataaacccgc tttcacgata ggaattgaag
    40981 aagagtttca aactgttgat cctgaaacgc gcgaattgcg ttcccatgtc agtggtcaaa
    41041 tgatcgagga aggtaaatta cttctttccg agcagatcaa aatggaaatg catcagtccg
    41101 tagtagaagt tggaacgaac atttgtcaaa acattactga agcaaaggcc gatgttataa
    41161 agctgcgccg taccattcat caacttgcca ccaaaagagg gctgggtatc gtcgctgcca
    41221 gcacacaccc atttagcgat tggaaaacac aacctattac ggagcacgaa agatacaaag
    41281 agatagtcga aaacatgggg gatgtggcac gtgcaaatct tattttcgga ttacacgtcc
    41341 atataggaat gccggataat gaatcgtgca ttgcgattca aaaccaaata agatactttt
    41401 tacctcatat tttagcactt tcgacaagtt caccgttttg gatcggtcga agtaccggct
    41461 taagctcaat caggactcaa atttttaggc gttttccacg aacggatatc ccaccgattt
    41521 tcgactcatg gtcagatttt gaagattatg taaacctctt aattcaaacg aaatgcattg
    41581 ataacggcaa aagaatttgg tgggatgcac gacctcatcc gagctttgga actttggagg
    41641 taagaatttg cgatttgcct acacgtgtag atgaaacgat agcaattgcg gctttgatac
    41701 aagcgctgat gcatacctta tacagaacgt acaagaaaaa catgtcctgg cgcatatatc
    41761 ccaatcaatt gatatctgaa aataaatggc gggcgagtag gtacggatta catggtaaat
    41821 tgattgattt tggtaaaaaa gaagaagttc cgacaaaaga actaatttat gaactaattc
    41881 gctttgtaga tgattctgtc gaagagcttg gaagtcgcaa tgaaattaac tatatttata
    41941 agattttaga tggcggcacc agttctgatc ggcagttggc tgtgtacgag cagtccggca
    42001 gggacttaaa agctgttgta gattttctat tacgcgatac tttggaaggc ttatattgac
    42061 aatttttatt catcataaat taatgtcaac ttattaaata attggaggtt atatgggtat
    42121 tttcacgcgt atggcagata tcgtaaaagc caatatcaat gactttttgg acaaagctga
    42181 agatcctgaa aaaatgatca aacaaatggt catcgaaatg gaagaagcag tcaataaagc
    42241 cacaacagca gttggaacag ctatcgctaa tcaaaaaaga ctcgaaaaac agtttaatga
    42301 aaacaaaaag ctagccgatg aatggcaaaa caaagccgtt caggcggtta atgccggacg
    42361 cgatgatttg gcttcacagg ctttggcaaa gaaaaactcg tatgcaacag ctgccgcttc
    42421 tttagagccg acattacaac aagctacagc aactgccgaa caaatgaaga ctcagttgca
    42481 acaattaaaa gctaaacttg acgaagcgcg tgtcaaacaa aatacattga tagctcgcca
    42541 ccaagctgca aaagctaaga aaatgattgc tcaacaaatg tccggtatag gcggcggagc
    42601 ttttggcaac tttgataagt ttgaaaaaaa ggttgaagat gttgaagctg aagcggatgc
    42661 ccatgttgaa ctttcaggac aaaatacaag tctagaagat gaatttaaaa agctatcaac
    42721 agacacaacc gtcgattcgg aattagccgc tttgaaagct caattaggaa aataaataat
    42781 atagtttgta gaattaaatt taaaggcgaa ctatgttgat tagttcgcct tttattatta
    42841 ttctaaaaat tagtatccat cacaaaattg gaaatgcatt ggatcacagt ttagatcagc
    42901 ccaatcacca ccccaaataa aaccattgtt tttaaatatt ttaactattg atggattcat
    42961 atttccttta gtaccaagtg cattagtagc cgggttgagg tctatggcaa ttccccaact
    43021 atgagtagat aatttgtttg ttgttctttt taatcgaaaa ttaaaacaac cgccaaaaag
    43081 ctctgctttc ggagcaagtc cggcttgata gatatcatta aatacttttt gaaaaacagg
    43141 gatcatatct ctgtggcaat acattctttt tattgttttt tcaggcatcc aagccaattt
    43201 aagaggatag ggtaatacgg cattccccat atacttcatt tcaaattgcg atgaaagtgt
    43261 cccattactc aagatatact ttcgtatatc accgaattgc tttacgatct cggcataccc
    43321 attaggcgtg ttcataatta ttgccattca tatatttaac aattcaaaat aaccaaccac
    43381 taaacggtta tcactaattg taaagatttt aacattgttt tatgtagtag caataagaat
    43441 tacttggata ttaaattaga gctttttatt gcgacatacg aattcgagta tttctaaaat
    43501 acacataatt tgtcttagaa tccagcaata aattaacttt taattgaaag atatatatac
    43561 caccatagca caacttagat atataatata tattatgtaa acttattaaa atccacttac
    43621 ttctttattt ttattataaa ttcagtccct cttccttgag ttgttttaaa ctcaagacgt
    43681 ccattgatct gctttataat cccaaaagct acgctgagcc cgagtccact acctttgccg
    43741 acatctttgg tagtaaagaa tggatcaaac attttttctt gtacttcttt tgatatgcct
    43801 aagccgttat ctgtaatgtt tatgttaatt tcttctggat tagcgaacaa acttattatt
    43861 atttttgggg tttctgattt gtttaaacga gccgcgtcaa taccattttc aataataccc
    43921 atgatagctt gatttagtaa tcctggattg caagcaagtt cagccgattc agtttgatcg
    43981 catttttttg aaaaattaaa tttaacatcc gtatattggc tttttaataa ataaatcatt
    44041 gtttcaagac tatcacacaa attgactgtt ttgaattcgg cagaatctaa attgctaaaa
    44101 tttttaagtt tattaacaat gtcaacaatt cgtttagaac cattattgca tgaagccagt
    44161 aatttatcta agtcattgat caaaaattga tagtcaatct gtgctttaat tgaattaacg
    44221 tattgttttt gttgatctac ggtgtcaaaa ttcttatccc aaacagatac taatgttttt
    44281 aaatcattga tatattccgc taatgattgc aggttaccat caataaaagt caaaggattg
    44341 tttatttcat gagcgatacc cgccgtcata gttccaagtg atgccaattt ttctttctgt
    44401 acaagttgca attctttctc ttctaattgt ttataagctt tttctaattc agatttacgt
    44461 tgacctaatt catattgcac ttgaaatgac tttttcctca aatgcacatg tatataagcg
    44521 ccggacaaag caaccatata caagaataac atcaccgact gacgatagac atagctatcg
    44581 gtttcgacat ctgtttcaag aaaataaata cacgcattgg acaaaaatat aatcacatat
    44641 aaatacagat attgtctgaa ggtgtaatta aaaaaagcta aaatatacac ggctattacg
    44701 atgtaaaccg gatacgttgg cgcttcatag cctcccaaac gtatgataaa aaaaggaaac
    44761 gtaatggccc aaaccgtgaa aagaatcaga gatatgtggg taacattttg cctgaatgaa
    44821 cggtaacgaa ataaaaacaa tagaagcgca cccaaaccaa tcaaagctaa attataatac
    44881 ggaacgcctc cccatccgct tataatccgt acaatgccga caatgaactg agttaaaatc
    44941 aaaaaccata taaatacgac tgcatttttc ctattttgat cattggctat tcgatgaaat
    45001 tcgttacgtg cattcggatt cgaatcaaaa aaacttgcaa gctccgattc atggaagccc
    45061 gtaatttttt ctaaaaatga aatcatagtt atgttatatg agatgtgtaa attggctgaa
    45121 ctaataaatg ataatcattt aatgtccagt tatcaaaata tttcttgtat attcagcatt
    45181 ctgatgttaa tttaatcgcc gtaggtcggt ttctcacact aaaataaaaa tatctttttt
    45241 catggaacaa gtaggcatta atagtaatgt atatgccggt ggtaagattt tccatatcca
    45301 aaccgctgga agctataatg atctcgttgt acaatctgaa atttttgaag ccggtcgcat
    45361 tatttctgtt caaaagaaac gtattactga tgttacagaa atgccagaag atgattttcg
    45421 agaattcgtc aaaaagtatc accatgaagt ag
//
LOCUS       DAOQGF010000011        44790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_346_length_44790_cov_6.406773, whole genome shotgun sequence.
ACCESSION   DAOQGF010000011 DAOQGF010000000
VERSION     DAOQGF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 44790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_346_length_44790_cov_6.406773"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /gene="tkt"
                     /locus_tag="PK937_03185"
     CDS             complement(<1..1832)
                     /gene="tkt"
                     /locus_tag="PK937_03185"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNH31604.1"
                     /translation="MAPNTLESLCINTIRTLSMDAVQQANSGHPGTPMALAPIAFTLY
                     DKFMKHNPGNPHWPNRDRFILSAGHASMLLYSTLHINGYGISLDDIKQFRQLHSKCAG
                     HPEHGLAPGIETTTGPLGTGVANSVGFAIAEKWLAQYFNRPGYEILNYSIFAVAGDGC
                     MMEGISGEAASLAGHLGLDNLIWIYDNNKITIEGSTSLAFSEDVMKRFEAYGWFVQHI
                     TDANDTNALSSAIERAQQTKGKPSFIMVDSHIGYGAPHKQDSHSAHGEPLGEEEIKAT
                     KQVYGWDPEKKFFVPDDVKAYSQFAVEKGSQHEQEWQKKFEAYRTAFPDLAKQFELIQ
                     KRELPEGWDADLPVFPADAKGVAGRDAGGQLINALAAKIPWLVGGAADLAPSTKTLIK
                     GAKSFSRDNRDGRNFHFGIREHAMGAIVNGMTLSGLHAYGSGFLIFSDYMRPTFRLAA
                     LMHQPSLFVFTHDSIGVGEDGPTHQPIEQVGSLRAIPNLEVIRPADANEVAEAWKYMI
                     RLKDKPAALILTRQALPTMDRTKYAPASGAQRGGYILADCKGKPDIILIGTGSEVQLC
                     IGAYEKLTAEGYKCRVVSLPCWSLFERQGKAYWEKVLPSDVRCR"
     gene            complement(1969..2772)
                     /locus_tag="PK937_03190"
     CDS             complement(1969..2772)
                     /locus_tag="PK937_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNH31605.1"
                     /translation="MIKKFMVPEDKKISLKDYDPDETLGYENDDSVKEEFKKLNLKMI
                     DLQELLYAEKEKSLLIILQAMDAGGKDGTIKHVMRGLNPQSCVVHSFKGPSEEERSHD
                     FLWRIHQKVPAKGYVCIFNRSHYEDVLVTRVHGWINDKTAKQRFNQINEFEELQAENG
                     VTIIKFFLHISKEEQRKRLQERLDDKSKHWKFSANDLKERAMWGQYMDAFEDVFNHTS
                     KKHAPWYVIPSNKKWFRNLATANILVDTLKSLDMKYPKPKQDFSNLVIE"
     gene            2872..5280
                     /gene="pheT"
                     /locus_tag="PK937_03195"
     CDS             2872..5280
                     /gene="pheT"
                     /locus_tag="PK937_03195"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNH31606.1"
                     /translation="MKISLTWIKDYVDIKLSPQELAHRLTMNGLNVEAILENDPFKGV
                     VLGKVLEVTKHPNADKLSLTKVDVGTDILNIVCGAPNVKAGQLAPIATIGTMMPGNFE
                     IKKAKIRGEESCGMICSKSELGFETGKSPGIWELDSTTPHTLGQSFAAFIGSGEVVLD
                     IDVTSNRPDCLHMLGMAREIAMIENTTIRMPDVRVIESSEKAEAHASIAIEDREGCTR
                     YAGRVIRNVTIGPSPDWLVKRLEAVGIRSINNIVDITNFVMLECGQPLHAFDYDHLAG
                     HKIIVRKSRKGDTFQTLDDKSHTLNDETVMICDGEKMIAIGGIMGGKNSEISDSTKNV
                     LLESAHFDGKRIRRSSKHLGIMSEASNRFGRGINPEGVIYAIQRAAQLMHELAGGEIL
                     AGVLDVNFSIIKPAVITLHSASVTRLIGQEIPDNDIADILTRLGFGVINNGNGSFIVT
                     APSHRVDIEIEENLIDEVARIYGFDRIRPSTGAYGHYHHEPPSGEKMMHQTRAALREA
                     GLSEVYTNAMVHPSSQLAVEPEGEKYFVRLMNPISEDMSVMRISMLPSLLEVVRGNLF
                     RKNDSMQIYEIGRTYRNEGDPLPAERNVLAGCIVGQSAPTHWATKPRAIDYYDLKGIV
                     DRIMGKFWLDSLQYNPYHSFVFAPNSVSISCLASDSQVLTLGRFGRIQNAVLRALDID
                     TTVWAFEIDYDVLRRVGKFRKTFDLISRFPFIRRDLALIVDEKMQAGAIQNAIRSKAG
                     AHLTNLEVFDVYHGNQVTQGQKSLAFALRFESVERTLTEDEIDATMQSIIAYVENQFG
                     AKLR"
     gene            5296..5742
                     /locus_tag="PK937_03200"
     CDS             5296..5742
                     /locus_tag="PK937_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31607.1"
                     /translation="MARASEKHSRTRSAGSLDLVEIRLKKLLQDLHDAYAEIGRLQEI
                     TQQADHQVTEAVAEKQRELDDKEKIVRRQQNLIKTKDAAYQELQDAYNALRREADELK
                     RGSESPAQTVEIRSEPKAMLDPEFRTRMRVQLESVLNKINELEKII"
     gene            5756..6115
                     /locus_tag="PK937_03205"
     CDS             5756..6115
                     /locus_tag="PK937_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNH31608.1"
                     /translation="MDKSSVKVNIYGSEYVIKGDDNADHIRAIADYVDQKMKEINKSG
                     AIKSPLKVAILAALNITDEFFKTSADQTKTIETMENKAQKMLDMLTQAPLPETLDLTP
                     EANEQSAAEEINLFSQK"
     gene            6213..7775
                     /gene="rny"
                     /locus_tag="PK937_03210"
     CDS             6213..7775
                     /gene="rny"
                     /locus_tag="PK937_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNH31609.1"
                     /translation="MEIAIFILAALVSAVAAFLFGQVYATKIAVAKRIREADEEAQQI
                     RKTAQSDAENLKKEKLIEVSDEWYKKRHEFDEQTKSTRIQIKNQQDELLKRERAVEQK
                     GDLLTRKEKDVDQKQQQVQTKLQEVTKKTEQLEAMIKNQNDKLETIARMTTEEAKQVL
                     MANMLEKAKEDAAKSIRNIKEQAQIQAKDQIKNIMLQAVQRTAMHHVTESTVTAFKLP
                     SNEMKGRIIGREGRNIRAFESITGCEIMIDDTPNTIMISGFDPIRREIARKTLEALIT
                     DGRIHPGRIEDVYEKARRDFDEHIMNTGEQALFEVGIHGAHVEIVKLLGKLKYRHQYG
                     QNLLQHSMETAAIAGMIASELGFDAQLAKRAAILHDIGFAADRSDQPHAVVGAELARK
                     FGENAVVQNAILFHHDDPMIAHPINVILYTANMLSKERPGAQKEVLNNYLKRLNKLEQ
                     IAMSFNGVREAFVVQAGREIRVMVDYATLDDNRAIQTADDIAQRIETEMEYPGQIKIT
                     VIREYRAVDIAK"
     gene            complement(7870..8976)
                     /locus_tag="PK937_03215"
     CDS             complement(7870..8976)
                     /locus_tag="PK937_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNH31610.1"
                     /translation="MKNYTHTTIPVRALASGQILNIDVFRFEGDPSVPSAYIQSGMHG
                     AELQGTAVIDFLIRFFTDHPPKGTVTLVPAANPYALNNKTGEYTQGRFDPTYGDNWNR
                     LYWDALNNEDGIDIENLVKTIRSDAQNSIQKILRHELIRKLEQQLQKPLTEMGYGRKL
                     ALTLQRMAVEADSVLDLHCASHSVRHVYAPEYARQTVRYLNIPYVLFIPNLFAGAMDE
                     AVFCPWWTLSQKLSVPCPVEAYTIELGNQEAYSAKDAHGDAMGIINYLVQRGTCDPIK
                     PKGRDSAESIFACRLQDYKTIFSPTGGLCEFSTVTGGIVPAQEPLAVILTKAGNEKIS
                     VALPYDCIPIVQYSSAALHQGVELLKVFSKWEVM"
     gene            complement(8978..10183)
                     /locus_tag="PK937_03220"
     CDS             complement(8978..10183)
                     /locus_tag="PK937_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent decarboxylase"
                     /protein_id="HNH31611.1"
                     /translation="MKALTAHHLTLLQEAADRVRKPFFAYDLDGLRSHAMTLRSEGVR
                     LWYACKANPLSAILRTMGACSFSADAASLGELRQILRYSGIAPKNILLTGPAKDEAFV
                     TEALNAGVCTIIVESVHQLEMLQQLASTTHKPIQVLLRLQLDWDEKSASVLGGSAITP
                     FGLPPEVWQSVSWKSFPHCDVLGCHVFQWSNIGDIEKLNFFWHRIAVESKRLMQHIGK
                     PLEVLDLGGGLGVPYHADARELRWIEVDDALQKIRKQYEIPSIWLELGRYAVGGFGNY
                     LTRVMDRKQVRGTELLILEGGVNHIARPALTHASFPVHTLKPSSAKTQSFKLHGPLCT
                     ALDDLGLHDLPEDIQRGDWLVFRQCGAYGFTESMPYFLCHDIPAEIIIENNQYAVVRE
                     SVPAENWLK"
     gene            complement(10180..10944)
                     /locus_tag="PK937_03225"
     CDS             complement(10180..10944)
                     /locus_tag="PK937_03225"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNH31612.1"
                     /translation="MKNDWKKILEQLERGEIRAATYDNGFWRANVAVKEAILSAFREG
                     KLIAMNRPTYGRYGGFVDKDSMIPRSFQPKQKIRLVPGGSSVRRGAYVAPGVVIMPPS
                     YINVGAYIDEQTMVDSHVLVGSCAQVGKRVHLSAGVQIGGVLEPVGSAPVVIEDDAFV
                     GADAVIVEGICVGTGAVIAPGVVLSRGVTVYDAVHEKELGRGARIPDLAVVVPGTRTL
                     SSSWAKERGLATACAIIVKYRDAKSNAALQLEEALR"
     gene            complement(10941..11831)
                     /gene="dapA"
                     /locus_tag="PK937_03230"
     CDS             complement(10941..11831)
                     /gene="dapA"
                     /locus_tag="PK937_03230"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNH31613.1"
                     /translation="MKNLQSVTLWTAIVTPFLDNGKIDFLSFERLLRRQEAAGNGVVI
                     LGSTGEGLALNDDEKREVVRFTGTCKLHVPVMVGVGGFNLDATQRWIEENNQHAFIHA
                     YLLVTPLYAKPGARGQCSWFAALLEASQKPCMLYNVPSRTGVKLHTSVLRELKDHSRF
                     WAVKEASGSVTEFQDYREAAPGAAFFSGDDGLTPFFAAAGAVGLVSVMSNVWPEASAE
                     FVYRCVNRDTGVLLPTWRTASDAMFLASNPVPAKALLHELGLITSAYVRPPLSMEDLP
                     DRNKLIMADSLVQRWMEQYG"
     gene            complement(11835..12530)
                     /locus_tag="PK937_03235"
     CDS             complement(11835..12530)
                     /locus_tag="PK937_03235"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNH31614.1"
                     /translation="MPKISLIGNGKTGGAVERLLPPDEIAAVFNSKNPPTVEALKNSD
                     VAIVFVPGHVLNDLVPVLIQSAVPVVCGSTGFEWTMDVRRAVENSGATWITASNFSMG
                     MQWMFRMAREFRRLESQWAGSEAEIFEVHHIHKKDKPSGSALSIRQHYGKQIPIHSDR
                     TGDVVGFHSLVLATASEKLMLSHHVEDRSVFARGAIWTAQKLIEKRSRPQDYPSGIFP
                     LEDVFETWLNEGQ"
     gene            complement(12512..13894)
                     /gene="lysC"
                     /locus_tag="PK937_03240"
     CDS             complement(12512..13894)
                     /gene="lysC"
                     /locus_tag="PK937_03240"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HNH31615.1"
                     /translation="MNDLIIAKFGGTSMGTAEAMRQSARIVIANNRIRVVVVSATSGT
                     TNALLKVYHDLCACDTVSADQALKKIEDRHRTMCHALELDSSAVDELIVAAQNHMSLF
                     SASENREVWLDAWLSFGERLASRIFGKMLFHLKNNVTEADARSFIITDEVFGKAEPQP
                     DLLKTACLKKLLPLCGEQVIVTQGFIGATTDGRTTTLGRGGSDYSAALLGEALSAKEV
                     LIWTDVPGIFTMDPNLVPAAQTIPKISFSEAAELANFGAKVLHPATLWPAIRKGIHVF
                     VGSTFEPEAGGTWIEPDVNDKPLVRAIAMRKHQTLITVSSLRMLNTHGFLAKMFGVLA
                     DHKLSVDLVTTSEVSVALTIDGTSLGSSGEKISEKQELLRDLRRFADVAIEENLTLIA
                     LVGNNLQQTPGIAARTFDAIAGLNVRLICQGASSYNMCFLMHQSDAPQAVSALHRAFI
                     ESEVVCLKSA"
     regulatory      complement(14011..14179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(14362..14811)
                     /locus_tag="PK937_03245"
     CDS             complement(14362..14811)
                     /locus_tag="PK937_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNH31616.1"
                     /translation="MSKGTLNKVIIIGRLGGDPELRYTPSGAPVATFNVATNESYKDK
                     EGKLIESTDWHRIVAWNKLAEICGQYLKKGSLVYVEGKIKTRSYDDKDGQKKYVTEIR
                     ADNMQMLGAKMDGGRGEASLPHPADMGGGMDHAADAGGSSGSDDLPF"
     gene            complement(14838..15911)
                     /gene="recA"
                     /locus_tag="PK937_03250"
     CDS             complement(14838..15911)
                     /gene="recA"
                     /locus_tag="PK937_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNH31617.1"
                     /translation="MADEMVLSDKDKKAALDRTLTDIVKEYGKGSIMKLGERTAMQVE
                     VFSTGSISIDAALGVGGIPRGRITEIYGPESSGKTTLSLHVIAEAQKVGGVAAFIDAE
                     HALDAIYAKRLGVDIDNLLVSQPDNGEQALEITERLVRSNAVDVIVIDSVAALVPRSE
                     IDGEMGDATMGVQARLMSQAMRKLTGAVSKSKTCVIFINQVRDKIGVMFGNPETTTGG
                     RALKFYTSIRVDIRRIGQIKAGENLVGNRTKVKVVKNKLAPPFKEVEVDLMYGTGISR
                     EGDLVDLASTMNIVQKSGTWFSFEDEKIGQGRENAKQYLTDNPVVLKKIETRVRQELG
                     IAPAATEKESPKPAPTASEKKSK"
     gene            complement(16079..16483)
                     /gene="ssb"
                     /locus_tag="PK937_03255"
     CDS             complement(16079..16483)
                     /gene="ssb"
                     /locus_tag="PK937_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNH31618.1"
                     /translation="MNKGSLNRAVLIGRLGRDPQVRYTPTGTPITSFSVATTQVFKNK
                     EGKLVQNTDWHRVVAWSRLAEVCGQYLKKGSLVCVEGPMKTRRWDDKSGGKHALTEVV
                     AETMQMLGHKNAKSSESEGVQEEMEILAEATV"
     gene            16883..18922
                     /locus_tag="PK937_03260"
     CDS             16883..18922
                     /locus_tag="PK937_03260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNH31619.1"
                     /translation="MLRSCFFIAIVCTVMYLGCAPQNVETLPDPLVHNRDSSVTPGQD
                     FFLHANGGWFKRNPIPESESSNGLWLTIGEETRAAVREICEKSAQETKAPKGSNKQKI
                     GDFYASGMDSAASEKAGISPLKSELDRIDAMKSISELAAVAARLHQINGDPIFSFNIY
                     KDDKKPTEYAVALNQGGLGLPDRDYYFKNDERTTGIRAAYQKHLFRNFEIVGESPDQA
                     RAFADHIFALEKNLAKASRTLEGLRDPYANYNKMSVAELSKLTPSFDWNVFFQNTGLK
                     NVDSVIVGQPEFFKEVENTVKNTSLDAWKAYLRWSVIGTYAAYLNQALVDENFNFYSK
                     TLYGVKQMKPRWKRVVERTNRALGEVIGQVYVEEYLPKGTKEKLLEIGNNIRDVCRER
                     IEKVSWMSDATRERALLKLQTMAMKVGHPDQWRDYSRLSVERDTYVANVMRANVYEFD
                     FMIGKYGKPVDRTEWNITPQTYNAYYDPTNNEIVVPAANIIVPGFGKTMPDDAILYGI
                     IGGSTFGHEIIHGFDDQGSQYDETGKLNNWWTKEDRARFEAKTKMLVTQFNNYVVLDS
                     VHLRGEATLGENIADLGGVELGYDAFKRSAQYKNNQKVAGLTPDERYFLAYAYAWMIQ
                     MRPEMLASRIMSDVHAPAQHRVIGPLSNNVKFYEVFGIKPGDAMYRPENERVSIW"
     gene            19371..22031
                     /locus_tag="PK937_03265"
     CDS             19371..22031
                     /locus_tag="PK937_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNH31620.1"
                     /translation="MKLSDPFKILTPNERWAPTQSQMDAFQNAYEKLLPPLVYKIRLA
                     VARWRDEGYQGASNTSKSLLNFWFNQEHLIGQSKFSFFFAQREAIESIIYLYEIARAK
                     DKYELMRFDSSQRVSTGMFDENWTRYVIKMATGAGKTKVMGMTLVWSYFHKLYEADST
                     LSKDFLVIAPNIIVLNRLRKDFDGLKMFFDEPFFPDNGYDDKDWKNDFQLTLHIQDDL
                     KPITESGNIFLTNIHRVFFNEAPEPNFETTFLGVKPKPDADTSKGLDLGKVLRSDKIK
                     GLVVLNDEAHHIHDNSLAWFKSIEDISNKLKLKNGNGISLQADYSATPKHNNGAIFVQ
                     TICDYPLVEAIKQKVVKSPVLPDEASRQKIQEKDSSDFVERYRDYMHLGYLEWEQQYE
                     ELKSQKTPILFIMTMNTKEADQAAAFLEASYPKMKNSVLTIHTNNSGEIKETATSKKD
                     KEELEALRKAADEIDKDHSPYKAVVSVLMLREGWDVKNVTTIVGLRPFGADSKILPEQ
                     TIGRGLRKMFSLDMPEKLVVVGTPAFLEFVESLKTEGVEFQYSPMGKGSKGKSPVIVE
                     VDKENPNKDLDVLDIPIPQMSPRIYREFKNLELIDVSKFKNEKVIQKKFNSDELKEIV
                     FHDIDGDFSHKTIYKETTPDYRNVISFFTSTILKDSRLVSGFNILYPKVESFIKYQLF
                     TKEVELSNPQTMRNLSEIQPKEILKTTFKKAIDDLTITDKGTAEVKNFISLKLTKPKV
                     AENQPFLVPKKSVFNKIIGENPFELEFSAFCENRFNDVVSFAKNTMGEGGINFKIEYQ
                     ATDGNIREYFPDFFVKIDNRTFFVVETKGRQDLDDILKIKRLASWCHDINNAQKEYTY
                     TPVYIKQEKWDDIKDNLKSFQDLKTIFEVK"
     gene            22028..23488
                     /locus_tag="PK937_03270"
     CDS             22028..23488
                     /locus_tag="PK937_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31621.1"
                     /translation="MKPWVTKALHYLEQSLGEIPQELNELDWKEDLSPNNEKLSRHLS
                     AFANHPGGGFLVFGIDNISAKPVGLTRESVSIILDRLSNLCRNGVDPVVVIDHIVHPF
                     RDVQLLIIHIKESAVKPVHIANKTMEESYIRTGSSTRKASRQEIGGLMLNSKTPVFEE
                     LHASKLKNAVEIMTALDFAAIYKLLKKPVPTNLAEIIRWLQDEKMIVQIEDDGFYITN
                     FGALSAAQNLNDFDNLSRKSIRLIKYEGNNKTVQSKEFPGSKGYAIGFEGLIQFIKGI
                     LPGSEIIKNALRAETSIFPEIALRELIANALIHQDFSIRGSGPMVEIYDDRVEITNPG
                     KLLPNKNIDRLIRTTPESRNEILAAAFRRYNICEERGSGFEKAVTAIELFGLPPLRFE
                     ETDGSFKVVMYAPKPFADMSDKERIEACYQHCIIQYYGQGGMSNSSLRKRFGMHDKQA
                     SQVSRLIKLAIETGRIKYKDPNNQSTKLNEYIPYWA"
     gene            23771..26011
                     /locus_tag="PK937_03275"
     CDS             23771..26011
                     /locus_tag="PK937_03275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH31622.1"
                     /translation="MNDTDKNKIIELIKAGEKLPKEFIYKLFADEEDVFLFWNGRKED
                     VTNIALPFHSIEHIDEPRREKDKAQASMFETDFRGRQLKGWTNKLIWGDNKLILSSLA
                     NGPMREEIEKEGGLKLIYIDPPFAVGADFGFNIEIGGETAEKKQSIIEEIAYRDTWGK
                     GISSYLSMMYERLKLMHNLLADDGSIYVHCDGRVNSYLRLILQDVFGTDNNLNEIIWQ
                     RTTAGKFILNNLPKNSDYILWFSKSSKYNYTPITSELSEDNKKTYIHDDEDGKGPYNT
                     QPLINPKDSDRPNLRYNYTDLSGKVWSHPPSGWRVSLEKMVQLDKEGRLYFTGKTLRE
                     KNYLNEREEKGKQISNMWTDINGNSIRYSDESVDYPTQKPEALLERIIKASSNEGDLI
                     ADFFCGSGTTAAVAEKLGRKWITTDLGRFSVHTARKRLIGVQRELQESGKDFRAFELL
                     NLGKYERQFFMDDLTNGKRKAKEDLYVDLILEAYKAKRIDGHAHLHGHKAGRFVNVGP
                     LDVPVTQSRLMDIFEECRTKLYTQVDVLGFEFEMGLTPQFIQELKEKGVSVTLKYIPK
                     DVFDKRAVEKGQVKFFDVAYLNTKEKIKGKSITIELTDFVTHYTQDDIEELQQSMRAG
                     STVVIEDGQIFKIEKEKNGIVTRTVLTKNWFDWIDYWAIDFNYEDKKEIIKIKNEKGE
                     IEEKWSGNYLFENEWQSFRTKKNPTLEFTSIAHEYKSAGKYKVMVKVVDILGVDTSKI
                     IEVSIK"
     gene            26507..26962
                     /locus_tag="PK937_03280"
     CDS             26507..26962
                     /locus_tag="PK937_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH31623.1"
                     /translation="MILKKAFLLLITASLATFLQAQQLLTKDQQEAQQTVVKMFEALS
                     KRDSVNLKAHCTADITLYEYGQVWNIDTLTHKAITKNTDPDFNRTNTFEFINTETDTH
                     TAWVTYRLKSTITKDSKQTTIQWLESVFLEKQKNQWKVKHLHSTLMSRN"
     gene            27217..28806
                     /locus_tag="PK937_03285"
     CDS             27217..28806
                     /locus_tag="PK937_03285"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNH31624.1"
                     /translation="MKTYPSIFNDVIGPVMRGPSSSHCAASLRIGRIGRDLMDGDIRE
                     VYIEFDPDGSLATTHKGQGSDMGLFGGFLGWEAYEERLPDYQQAVDEAGIDIKIAIHP
                     IGATHPNTYKITLSNASETRELTAISTGGGMIEVIEIDGAQVRMAGDFYQTLVYVKSP
                     GEIISFIEKSFPCDEIGLRKGKSTFIEIKSNQFLPDPLCRELQSIDDVLFIKKLNPVL
                     PVMSRKDLTVPFITCEEMLAYNKGKNLELWELAVDYESMRGNITRQEVFEKMRAIVEI
                     MQNAIALGLKGTTYGDRILGAQSVEFQQQMKDKKLIEGDVLNRVILYTSAMMEVKSSM
                     GVVVAAPTAGSCGALPGAVIGLSDALHFSQDEMVKAMLAAGIIGVFIAAHATFAAEVG
                     GCMAECGSGSGMAAAGIVGIKKGTLDQALAASSMALQSSLGMICDMIADRVEAPCLNR
                     NVMAATNALSCANMALSNYNHLIPLDEVIETMKKVGDAIPNTLRCTGLGGLAITKTAK
                     KIEATLEKNQAESNKKFFKVC"
     gene            complement(28928..29134)
                     /locus_tag="PK937_03290"
     CDS             complement(28928..29134)
                     /locus_tag="PK937_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31625.1"
                     /translation="MPETKPVLRGVFFALYDFHISAKSLKSNAITLQNSDSNVTRFTG
                     IQVLHNAGFSLMCAADDNTGVSVF"
     gene            29298..29558
                     /locus_tag="PK937_03295"
     CDS             29298..29558
                     /locus_tag="PK937_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNH31626.1"
                     /translation="MSKLEKILLTVLRGTSDTNIDFEDLRKLLHTLGFSERIKGSHHI
                     FYKDGVVEIINLQSKNSKAKPYQVKQVRNLIIKYKLGGDENG"
     gene            29551..29760
                     /locus_tag="PK937_03300"
     CDS             29551..29760
                     /locus_tag="PK937_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNH31627.1"
                     /translation="MDKYEIILFWSEEDNSYVAEVPELPGCAADGATYHEALTNLEVS
                     IKEWIETAKVLGRDIPKPKGRLMYA"
     gene            30010..30447
                     /locus_tag="PK937_03305"
     CDS             30010..30447
                     /locus_tag="PK937_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH31628.1"
                     /translation="MASINPHINFNGNAEEAFNFYKSVFGGAFAKIIRFKDLASADFP
                     VAEHEANKIMHIALPIGKSILMANDVPEILGRTNENENRSKIVVSAESKEEADKIFHG
                     LSAGGQIEMPISDSPWGSYFGMFRDKYGIEWMVDFDARYKGQI"
     gene            30546..31427
                     /locus_tag="PK937_03310"
     CDS             30546..31427
                     /locus_tag="PK937_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNH31629.1"
                     /translation="MSAAKISIRFFNDREVRAVWDEQNAKWWFSVLDIVAVLTDQDDY
                     TKARNYWKYLKAKLKKERSQVVSATTQLKLLAPDGKKRMSDMLDYNGIIALGKTFPGT
                     RANRFIEWFTYSNESIDGKSKTKAYALFESSFINSIEVGTTKGLQQIHAYLFGGLYEF
                     AGQIRKKNISKSGFRFAVSGFLGETLKQIEKMPETTFDEIVNKYIEMNIAHPFMEGNG
                     RSARIWLDLILKKRLKKCVDWSQISKRDYMNAMMQSPTKNDALKKLLKKALTTKINDR
                     EMFMKGIDYSYYYEEND"
     gene            31765..32817
                     /locus_tag="PK937_03315"
     CDS             31765..32817
                     /locus_tag="PK937_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNH31630.1"
                     /translation="MPLRYMLAFFCVSALLFGQDKKPSIAVLDFKASEGLTRGETATL
                     TNRFRGILVQTEAFNVVEQEKMNEILKAQDFSMSDACNTAECAVQVGQLLGVERMIAG
                     DIGKIGDVFTIDLRLIDVSTGKIVKTVTRDYEGKISGLLGVMKDVASTFAAIKPVSPA
                     VGSITIQSTPSKATVSMDGKELNFVTPFVAENLTLGVHEIALRLGTMKAVKTIEIKEG
                     KNPVLNLRLEPITAPLSVASDPAGATVFVNGDRYGDTPLQIKLPIGSHTLTVKQKEYE
                     VFTQSIVLKENDPPLVIQASLTKKIKTLSESSSNKKWWYILGGAAVLGGGTAWLLSGG
                     GKSSGKSGIPPAEFPN"
     gene            32861..38269
                     /locus_tag="PK937_03320"
     CDS             32861..38269
                     /locus_tag="PK937_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH31631.1"
                     /translation="MRYTITIAAVLARVFFCVTLLPSFIFSQGGAWSSKTSMTTARWH
                     SGIGVINNTLYVAGGYNGSHQTVVEAYNFTSNTWTTKNTRPVQTSAAYGVIGNTLYTA
                     GGTNVSVRISDVWAYDPGLDSWTTRAPIPNLRSNPGSAVVNNMLYAIGGVDNSSVLSS
                     MDAYDPSGNAWASKAAMSVPRQAPAVGVIQGIIYVAGGFTTGNAVTDVLEAYNPVTNT
                     WSTKAVLPTARGYAVAAVLDDKLYVMGGVTVGGGSAIQTVEVYDPVTDTWSGGTPMKT
                     GRAGMEAFTYDNKIYAIGGWSGVSTASSILEAFNGRPVIFSFTPAFSPVDSIVTITGN
                     NFSAVPQENVVYFGSVKANVNTASITQLTVTVPAGQNFKPISVTVGGYTAFAKKSFFV
                     TKKVKSTIDESSFDTRIELTSGNNPKNVASGDIDGDGKPDLIISNFDDNTISVYRNIS
                     STGTLSAGSFSGPVDFNTSLSPHGMKIADMDGDGKLDVILSHSSSSFVTVLRNTATSG
                     VINASSFASAISFASGGGSQELTVSDIDGDGRPDIIAANTNGTISVFRNRNTPGSIGT
                     QGFAPYIDLAAGANSESVSAADIDGDNKPDIIFTNRNDNTLSIYRNQSTPDTLTASSF
                     SGLVNFTAATGPGKIAVADFDGDLKTDIATVNPGSATFSVFRNTATPGLINTSSLADR
                     VDFMPGAYQISLAAGDMNSDLKPDIISVAATLSKNNTSAGSITSGSFSTAFTFNVGTQ
                     PNDVELADLDGDGLLDVSMLDYVDKKLYIYRNAMAIVPRMADSLALVDLYDSLNGNGW
                     TNKTNWKSGNPIDTWFGITVTDGRVSHVELGNNNLSGFIPSSVGNISAIKNLNLFNNN
                     VGGPIPATISALTEIVSLNLQNNQLTGSIPASITALTKLKHLYLDFNTLSGAIPSDID
                     NLTDLETLSLYQNQLSGTIPGGIWNLHKLNHLALGPNPGLSGTLPAQIENLDSLQSLF
                     ISSCGLTGSIPPEIGNLNKILMIYASDNQFTGTIPASIGNLSLLRTLHLANNNLSGSL
                     PSSLANLHRLESLDIKENKFTDLGDLNPVDSLNVLMIEQNQFTFEDIEPNITAPRQSF
                     TYAPQDSIGTYKDTAVTENAATSLAVSVGGSGNQYLWFKDGSSTGITTPVISFGSFAF
                     ANAGTYHCQITNTVATALTLYSRTYTVKLRGPSIPTGLTASAQSASQINLSWSASQNN
                     PIRYRIYRSPTSGSGFVQVDSVSHPTVAYNNTGLTADTDYYYRITAVDAAGLYSAATT
                     QASARTLQTAVSITSVSLLVGPTQPNENTIVTISVPVSGSAPVVKLYYAKNSSVNPDS
                     TEMTLSSGPYVASIPASFVTAQGLWFRIRAYNNTGSEFYPSSTAKHPINVKAGASTLS
                     AIRSSGAYPSGMIADGYSTFGLPMDTSVGIPALFGSQIFNSKNEPTNWRIQKYADGSF
                     TDETSAFTSGTGYFVYHRLGSSVQVDIPPGSLTHAAGTFENFVLKPGWNLVPWPYTFS
                     ANFTITDESAIDPIQTQIGGNWQIVSSFKPFGAYAIKNTTASDILAGDVIQWTLSTSK
                     VTVDYDLISDSFVRLSAVSGRYLDNDNFAGFSDAARDGDDPLDNEEPPAIGPGVNLYF
                     TNGTEQRQYADIRSRGDGHVWDFHVNDRNNGKRINLAWDLAHIPDRMSAVLINIAENK
                     WINLGEQTSYAFSPVKENRFKLIIGTSDFVQQTSIALAKELPSSFALHQNHPNPFNPS
                     TTIRYDIAHAGHVRIKIYNLLGQEIVTLVDQFVNTGRYSKLWDGRDRLGNKVASGIYI
                     YRLETSKFVRSKKMLLMK"
     gene            complement(38356..38829)
                     /gene="ssb"
                     /locus_tag="PK937_03325"
     CDS             complement(38356..38829)
                     /gene="ssb"
                     /locus_tag="PK937_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNH31632.1"
                     /translation="MNKGSLNRAVLIGRLGRDPQVRYTPTGTPITSFSVATTQVFKNK
                     EGKLVQNTDWHRVVAWSRLAEVCGQYLKKGSLVCVEGPMKTRRWDDKSGGKHALTEVV
                     AETMQMLGSRSSKQNVHDDDASDETGIETHDDVAEIVSENEYSEHTVVRTDEVPF"
     gene            39015..39857
                     /locus_tag="PK937_03330"
     CDS             39015..39857
                     /locus_tag="PK937_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HNH31633.1"
                     /translation="MINSIDDLQRQIEIAALIYEFPDKYSEIDLAEKFHTSESSIHRD
                     MRALRAMGITVNSRKRAFRIELSLLQINTLITTYFAFGNNETIKNLPLVLERFQNKTL
                     SFFVETIRAIRQKHVIEIEYRSAKTNRYQWRTVTPVTFYNAGKTHYLIAIHQDTAKMF
                     TIERIQNFRFSRQASSIKNVPTLNELFRHSWGSFTGGKLTDVHLRFKDDLGQYMAEKF
                     WIEEQAMKQSDNGFEIRLKVKLSNEFIAWILGWGDAVTVLAPDELRQSVLEKAKSIVN
                     HYKK"
     gene            39985..40764
                     /locus_tag="PK937_03335"
     CDS             39985..40764
                     /locus_tag="PK937_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH31634.1"
                     /translation="MGRIIAIANQKGGVGKTTTSINLSASLAHAGKRTLIIDMDPQAN
                     STTGLGFDLEKIERGSYEVLIQTVPATDTILKTMMPSMDLLPAHIRLVGAEIELVNLE
                     EREHMFKKAIAPIRDQYEYILIDCPPSLGLLTLNALSAADTVLIPIQCEYYALEGLRQ
                     LLNTIRLVQRHLNTDLTIEGVLMTMYDSRLNLSNQVVGEVKEYFKDKVFKNVIHRNIK
                     LGEAPSFGKPIILYDPASVGSQDYMGVAKEVIANVITPQPA"
     gene            40777..41706
                     /locus_tag="PK937_03340"
     CDS             40777..41706
                     /locus_tag="PK937_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNH31635.1"
                     /translation="MNFTPKKNTGLGKGLSALIREELNADGTLKDEVRSDTPIAKIPI
                     DQIRANPFQPRKEFEPQALEELKQSILQNGIIQPITVRKTATGYELIAGERRFRASKL
                     AGLTELPAYIRENVTDQEMLELAIIENVQREKLNPIELAEGYQLLIETCNLTQDQVAQ
                     KIGKDRTTITNIIRLLKLPADIQESLKKEEITIGHGRALIALPSEQLQLKAWKQIVDG
                     GMSVRKTEQLVKTTLEELEGKSHKVSPNKYEIESAQPANPHIADVEGRLRMKLGTKVR
                     LHHHKDGSGSVEIEYYSNDDLQRVIEMIETIRG"
     gene            41709..41873
                     /locus_tag="PK937_03345"
     CDS             41709..41873
                     /locus_tag="PK937_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31636.1"
                     /translation="MGVGEHIRKQIGQSKKRPTELVAELNEFLKKIGKKPKQENNERI
                     RKIVGPADKK"
     gene            42313..43212
                     /locus_tag="PK937_03350"
     CDS             42313..43212
                     /locus_tag="PK937_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH31637.1"
                     /translation="MPCTPVTVRTILNKTKRRDPWFLDDYTLNPYSGCSMGCVFCYVH
                     GSRYGTHTEKKLSYKTNAPELLDKQLSLRAKRGQYGFIVLASSTDPYVHAEKELGLTR
                     RLLEIILHHRFPVHIITRSDLILRDTDILMHIGKTAILPEDLLGKVPSGALITFSFSS
                     VDDRTAHIFEPGATPPSRRLQGLKQILDEGFYAGVSLMPLLPWISDTADQLELSFSTF
                     AQIGARYIFPASLTLYGDQPSDSKIRTLRTIQQHYPHLMDKYRGYFDRSATMPDYYLT
                     ALEEKLNGLCEKYRLPNKVHLND"
     gene            complement(43223..>44790)
                     /locus_tag="PK937_03355"
     CDS             complement(43223..>44790)
                     /locus_tag="PK937_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III deoxyribonuclease"
                     /protein_id="HNH31638.1"
                     /translation="QKTSTVAIVQKAFRFFEAKGISANEIVKRIQANFKPENCLSANN
                     DAEAEQNQILLNTLEDENNPIRAVFAVQKLNEGWDVLNLFDIVRLYEDRDGKDGKPGI
                     TTLSEAQLIGRGARYFPFAIEEGHDVFTRKFDDDISNDLKTLEELYYHTKEDSRYISE
                     LKKALVDSGIYEDEENLVTKQLRLKIDFKETEFYRDGHVFFNKKILKRSDNIKSFADL
                     GVKRTNYRHILSSGVGRMSSAFFEMEPTQSGEEIIKSKDVKLTDIPKNTIRFALSQNP
                     FFYFDSLSYYFPSVGSLSNFIDSKELLASLEITFNGTANRLKEINHFDYLQALNGLLQ
                     SIEADIKSNSTEYEASDYIKEPIHKVFKDKEIKVYKDSVRADGQETLVANEPWYVYNA
                     NYGTSEEKKFVELFSRRFEGLNLKFENIYLIRNEREIKIFDKLGRAFEPDFLLFCKQR
                     DGEQMTFQVFIEPKGNHLLSYDKWKEDFLKEIRNEQKAIKIHTDTYLITAVPFYNYDN
                     ENEFRITLENTLK"
BASE COUNT        12746 a        10605 c         9884 g        11555 t
ORIGIN      
        1 atgcggcaac gcacatcgct cggcagtact ttttcccagt acgccttgcc ttggcgttca
       61 aataaactcc agcacggcag actcaccacg cggcatttat aaccctccgc cgtcagcttt
      121 tcataggccc cgatacacag ttgtacctcg gaacccgtac cgatcagaat gatatccggt
      181 ttacctttgc agtcggctaa aatgtatccg ccgcgttgcg caccggaagc cggtgcgtat
      241 ttagtgcgat ccatggtcgg taaggcctgg cgtgtcaaaa tcagagccgc cggtttatcc
      301 ttaagtcgta tcatatattt ccatgcttcc gctacttcat tggcatccgc cggacggatc
      361 acctcaagat taggaatagc gcgcaacgaa ccgacttgtt cgatcggttg gtgggtcggg
      421 ccgtcttcac cgacgccgat actgtcatgc gtgaaaacaa acaacgacgg ctggtgcatc
      481 agcgccgcca accgaaatgt gggtcgcata taatccgaaa aaatcagaaa acccgaaccg
      541 tacgcatgca acccgctgag tgtcatcccg ttgacgatgg cgcccatggc atgttcacga
      601 ataccaaagt gaaaatttcg cccgtcgcga ttgtcacgac taaaactttt tgcgcctttg
      661 atcagagttt tcgtagaggg tgcaagatcc gcggcgccac cgacaagcca gggtattttg
      721 gccgctaagg cattgatcaa ttggcctccg gcatcacgcc ccgcaacgcc tttggcgtcg
      781 gccggaaata ccggcagatc ggcatcccaa ccttcgggta attcccgttt ctgaattaac
      841 tcaaattgtt tggccagatc cggaaacgca gtgcggtaag cttcaaattt tttctgccat
      901 tcctgctcgt gttgcgaacc tttttctacg gcaaattggc tgtatgcttt tacgtcatcc
      961 ggcacaaaga attttttttc gggatcccaa ccgtaaacct gtttcgtcgc tttaatctcc
     1021 tcttcgccga gcggttcacc gtgcgccgag tgcgaatcct gtttgtgcgg tgcaccgtat
     1081 ccgatgtgac tatccaccat gataaacgac ggtttacctt tggtttgctg agcgcgctcg
     1141 atggcggacg ataacgcgtt ggtatcattg gcatcggtga tatgttgcac aaaccaaccg
     1201 tacgcttcaa aacgtttcat cacatcttcg ctgaacgcca gcgatgtcga accttcaatc
     1261 gtaattttat tgttatcata gatccaaatg agattatcca atccgaggtg gcccgccagc
     1321 gatgcggctt cgccggaaat gccttccatc atacatccgt caccggccac ggcaaaaatg
     1381 ctgtaattca aaatttcata gccggggcga ttgaaatatt gcgccaacca tttttcagcg
     1441 atggcaaagc cgaccgaatt cgccacgccc gttcccaaag gccccgttgt cgtttcgatg
     1501 cccggagcca gaccgtgctc gggatgcccc gcacacttgg aatgcagttg tctaaactgt
     1561 ttgatatcgt ccagcgaaat accatacccg ttgatgtgca gtgtactgta aagcagcatg
     1621 gatgcatgcc ctgccgataa aataaaacga tcccggttgg gccaatgcgg attgcccgga
     1681 ttgtgtttca taaatttatc atacagcgta aacgcgatcg gcgccagagc catcggtgtt
     1741 ccgggatggc cggaattggc ctgctgtacc gcatccatag acagtgtacg aatggtatta
     1801 atacacagcg attcaagggt attcggagcc atgaagtttt tctcccaaag gtatgattga
     1861 aaggacgccc caaagttaca ctatccgttg cgatagatca acggaatatt ttataacaca
     1921 aatccccggt ttatgcccgg ggatttgtga aaaaaaatca aactaatttt attcgatcac
     1981 aagatttgaa aaatcctgct tgggtttggg atacttcata tcaagggatt tgagcgtatc
     2041 taccagtata ttggccgtag ccaaattacg aaaccatttt ttattggaag gaatcacata
     2101 ccacggcgca tgttttttac tggtgtggtt aaatacatct tcgaatgcat ccatgtattg
     2161 tccccacatc gcacgttctt tgagatcgtt ggctgaaaac ttccaatgtt tggatttatc
     2221 gtccaaccgt tcctggagac gcttgcgctg ctcttctttg gaaatatgca gaaagaattt
     2281 gatgatcgtg acaccgtttt ccgcctgcaa ctcttcaaat tcgttgattt gattaaaccg
     2341 ctgttttgct gttttatcat tgatccatcc gtgaacgcgc gtcacaagca catcttcgta
     2401 atgcgaccgg ttaaaaatac atacataccc cttcgccggc actttttggt gaatacgcca
     2461 taaaaaatca tgcgaacgtt cttcttcgct cgggcctttg aagctatgca ccacgcaact
     2521 ttgcggattg agtccgcgca tcacgtgttt gatcgtacca tctttaccgc cggcatccat
     2581 ggcttgcaat atgatcagaa gtgatttttc tttttcggca tataagagtt cttgcagatc
     2641 aatcatcttc agattgagct ttttaaactc ttccttgacg gaatcatcgt tttcgtaacc
     2701 gagggtttca tccggatcat agtccttgag tgaaattttt ttatcttcgg gcaccataaa
     2761 tttcttgatc atgacacgct cctcaggtta aaaacccttg attaaaatac atttaggtca
     2821 tattcttgca ttacgcaaat atacgatcac gtttcaatat acggagtttt catgaagatt
     2881 tccctgactt ggatcaagga ttatgttgat atcaagttgt ccccgcaaga gctggcccat
     2941 cgtcttacga tgaatggact taatgtcgaa gccattttgg aaaatgatcc tttcaaaggt
     3001 gtggtcctcg gtaaagtgct cgaagtaacc aagcatccca atgccgataa actgtcgctc
     3061 accaaggtag atgtcggcac ggacatactg aacatcgtct gcggagcgcc caacgtcaaa
     3121 gccggccaat tggcccccat cgctacgatc ggcacgatga tgccgggaaa ttttgaaatc
     3181 aaaaaagcaa aaattcgcgg cgaagaatcc tgcggtatga tttgctccaa aagcgaattg
     3241 ggatttgaaa ccggcaagtc gcccggcatt tgggaacttg attctacaac gccgcatacc
     3301 ttaggtcaat cgtttgctgc gtttatcgga agcggcgaag tcgtactgga tattgatgtg
     3361 accagcaacc gtcccgattg cctccatatg ctcggcatgg cacgcgagat cgccatgatc
     3421 gaaaatacga ccatccgtat gcccgacgtg cgtgtcatcg aatcctcgga aaaagccgaa
     3481 gcgcatgcgt ctatcgctat cgaagatcgc gaaggctgca cacgctatgc cggacgcgtc
     3541 atacgcaacg tcacgatcgg cccctcgccg gattggttgg tcaaacgact cgaagccgta
     3601 ggtattcgtt cgatcaacaa catcgtggac attaccaatt ttgtcatgct ggaatgcggc
     3661 caaccgctgc acgctttcga ctacgatcat ctggccggcc acaaaatcat cgtgcgtaaa
     3721 tcccgcaaag gcgatacgtt tcaaacgctg gatgataagt cgcacacact caatgatgaa
     3781 acagtgatga tctgcgacgg cgaaaaaatg atcgctatcg gcggtatcat gggcggaaaa
     3841 aattcggaaa tttcggattc gaccaaaaat gttctcctcg aatcggccca ttttgacgga
     3901 aaacgaattc gccgctcatc caaacacctc ggtatcatgt cggaagcatc caatcgtttt
     3961 ggccggggca tcaaccccga aggcgtgatc tacgccatcc agcgcgccgc acaactgatg
     4021 catgaactgg ccgggggcga aattctcgcc ggtgtattgg atgttaactt ttcaatcatc
     4081 aaaccggcgg tgatcacact acactcggca tcggttacgc gccttatcgg tcaagaaatc
     4141 cccgacaacg atatcgcgga tattcttacg cgccttggct ttggcgtcat caataacggc
     4201 aacggttcat ttatcgtcac cgcgccatcg catcgcgtag atatcgaaat cgaggaaaat
     4261 ctgattgatg aagtggcccg catttacggt tttgatcgca tccgtcccag taccggcgcc
     4321 tacggacact atcatcacga accgccttcc ggcgaaaaaa tgatgcacca aacccgcgcc
     4381 gctttacgtg aagccggttt atcggaagta tataccaatg ccatggtaca tccttcatcg
     4441 cagctggccg tcgaaccgga gggtgaaaaa tattttgtgc gcctgatgaa ccccatcagc
     4501 gaagacatgt ccgtcatgcg catttccatg ctgccgagtt tgctggaagt cgtacgcggt
     4561 aatctatttc gtaaaaacga cagtatgcag atctatgaaa tcgggcggac gtaccgcaac
     4621 gagggcgatc cactgccggc ggaacgtaac gtattggccg gttgtatcgt cggccaaagc
     4681 gcccctaccc actgggcaac caaaccccgt gccatagatt attatgatct taagggcatt
     4741 gtcgatcgga ttatggggaa gttttggctt gattcgctac agtataatcc ttatcattct
     4801 ttcgtatttg cgcccaattc ggtttccata tcctgtcttg cttcggattc tcaggtatta
     4861 acacttggtc gctttggacg tatacagaat gcggtcttac gggcattgga tattgacacg
     4921 accgtttggg ctttcgaaat agattacgat gttttacgac gtgtcggaaa attcagaaaa
     4981 acatttgatc tgatttccag atttccattt attcgccgcg acttggcctt gatcgtggat
     5041 gaaaaaatgc aggccggcgc gatacagaat gccatacggt ccaaggccgg tgcgcatttg
     5101 accaacttgg aagtatttga tgtgtatcac ggcaatcagg taacacaagg tcaaaaaagc
     5161 ctcgcgtttg cgttgcgatt cgaatccgtc gaacgtactc tgacggagga tgagatcgat
     5221 gcgaccatgc aatccatcat cgcgtatgtc gaaaatcaat ttggcgcaaa actgcgataa
     5281 caggtaataa caaaattggc gcgagcatcc gaaaaacatt cacgtacacg cagtgccggc
     5341 tcccttgatt tggtggaaat ccggttaaaa aaattgctac aggatctgca tgatgcgtat
     5401 gcggagatcg gacgtttgca ggagatcacg cagcaagcag accatcaggt gacggaagcc
     5461 gttgccgaaa aacagcgtga acttgacgac aaagaaaaaa tcgtccggcg gcaacaaaac
     5521 ctgatcaaga ccaaagatgc ggcgtatcag gaattgcagg atgcgtataa cgcccttcgc
     5581 cgtgaggccg atgaactaaa acgcggcagt gagtccccgg cacagaccgt ggaaatacgt
     5641 tctgaaccta aagcgatgct ggatcccgaa ttcagaacgc gcatgcgggt gcaattagaa
     5701 agtgttttga ataaaataaa cgagttggaa aaaattattt aattttgcga taacagtgga
     5761 taagagttcc gtcaaagtca acatctacgg cagcgaatat gtcatcaaag gcgatgataa
     5821 cgccgatcac attcgcgcaa ttgccgatta tgtagaccaa aagatgaaag agatcaacaa
     5881 aagcggtgcg atcaaatccc cgctcaaagt ggcgatcctt gcggcgctga atattacgga
     5941 cgagtttttc aaaaccagcg ccgatcaaac gaagacgatt gaaacgatgg aaaacaaggc
     6001 tcagaaaatg ttggacatgc tgacgcaggc tcccctgccc gaaacgctgg acctgacacc
     6061 tgaggctaac gaacaatcgg ctgccgaaga aatcaatctt ttttcgcaga aatagtcttc
     6121 actctgacat tatcccctgt tgtcctttgg cgcacagcgc ccgaaggtca cagggttttt
     6181 ttatagatca acggagttag atcaagacaa tcatggaaat tgctattttt attttagcgg
     6241 cgctggtatc cgccgtagcg gcctttcttt tcgggcaagt gtacgcgacc aaaatcgccg
     6301 tggccaaacg cattcgcgaa gccgatgaag aagcccagca aattcgcaaa acggcccagt
     6361 ccgatgcgga aaatctcaaa aaagaaaagc tcatcgaagt cagcgatgaa tggtataaaa
     6421 aacgccatga gtttgacgag caaaccaaaa gcacgcgcat tcagatcaaa aaccagcaag
     6481 atgaattact caagcgcgaa cgtgccgtag agcaaaaagg cgatttgctg acgcgtaaag
     6541 aaaaagacgt cgatcagaaa caacaacaag tgcagacaaa acttcaagaa gtcaccaaaa
     6601 aaaccgaaca acttgaagcg atgatcaaaa accagaacga taaactggaa accatcgcac
     6661 gcatgacgac ggaagaggcc aaacaggtgc tgatggccaa tatgctggaa aaggccaaag
     6721 aagatgcggc caaatccatt cgcaatatca aagaacaagc gcaaatacag gccaaagatc
     6781 agatcaaaaa tatcatgttg caagccgtac aacgcacggc catgcatcac gtgaccgaaa
     6841 gcacggttac tgcatttaaa ctgccgagta atgaaatgaa aggccgtatc atcgggcgtg
     6901 aagggcgtaa tatccgtgcc ttcgaatcca ttacgggttg cgagatcatg attgacgata
     6961 cgcccaatac gatcatgatt tcgggatttg atccgatccg tcgtgaaatc gcacgaaaaa
     7021 cgttggaagc actgatcacg gacggacgta ttcatccggg tcgtatcgaa gatgtgtacg
     7081 aaaaagccag acgggatttc gatgaacata tcatgaatac cggagaacag gcgctttttg
     7141 aagtcggtat acacggcgcc catgtcgaaa tagtcaaact gctcggcaaa ctcaaatacc
     7201 gtcatcagta cggacagaat ctcctgcaac atagtatgga aacggccgcc attgccggta
     7261 tgatcgcatc cgaattaggt tttgacgcac aacttgccaa acgcgctgcg atactgcatg
     7321 atatcggatt tgccgccgac cgctccgatc aaccgcatgc cgtcgtgggc gccgaactgg
     7381 cacgtaaatt cggtgaaaac gccgtcgttc aaaatgccat tttatttcat catgacgacc
     7441 cgatgatcgc ccaccctatc aacgtgattt tgtacacggc gaatatgctg agcaaagaac
     7501 gtccgggcgc acaaaaagaa gtgctgaata attatctgaa gcgcctcaat aaattggaac
     7561 aaattgcgat gagcttcaac ggcgtacgcg aagcgtttgt cgttcaggcc gggcgggaaa
     7621 tacgtgtgat ggttgattat gcgacactcg acgacaaccg cgctattcaa acggccgacg
     7681 atatcgcaca acgtatcgaa accgaaatgg aatatcccgg acagatcaaa atcacggtga
     7741 ttcgcgaata ccgtgccgtg gatattgcaa aatagtattc tctgacaatt taaaagatta
     7801 tatccctcat cggtattaag gcatgccaat catccttgtt tcatgaggga tttttattgt
     7861 tgtccgaatt cacattactt cccatttcga aaacactttg agcaattcga ccccttgatg
     7921 caaagccgca gatgaatatt gtacgatcgg gatacaatca tagggtaatg ccaccgatat
     7981 tttttcattc cccgctttag tgagtataac cgccagcggc tcctgtgccg gaacgatccc
     8041 accggtaacc gtactaaatt cacacaatcc tccggtcggg ctaaagatag ttttataatc
     8101 ttgtaaacgg catgcgaata tggactccgc gctatcacgg ccttttggtt ttatcgggtc
     8161 gcatgttccc ctttgcacta aataattaat aatacccatc gcatcgccgt gcgcatcttt
     8221 tgcggagtaa gcttcctgat tgccgagttc gatcgtatag gcttcaacgg ggcacggcac
     8281 acttagcttt tgacttaatg tccaccaagg gcaaaatacc gcttcatcca ttgcaccggc
     8341 aaaaagattg ggaataaata aaacatacgg aatattcaaa tacctcacgg tttgacgcgc
     8401 atactccggc gcatacacat ggcgtaccga atgcgaagcg caatgaaggt caagcacact
     8461 atccgcctcg acagccatac gttgtaaagt caacgccagt ttgcggccat aacccatttc
     8521 cgtaagcggc ttctgcagtt gctgttccag tttacggatc agctcatgtc gtaaaatttt
     8581 ttgaatacta ttttgtgcat cgctgcgaat ggttttaacc aagttttcta tgtctatgcc
     8641 gtcttcatta ttcaaagcat cccaataaag acgattccaa ttatcgccat acgtcggatc
     8701 aaatcttccc tgcgtatatt ctccggtttt attatttagt gcataagggt tggccgccgg
     8761 gacaagtgtc accgtgcctt tgggcgggtg atctgtaaaa aaacgaatta aaaaatcaat
     8821 cacggcggta ccttgtaatt ccgcaccgtg catgccggat tgtatatatg ccgacggcac
     8881 agacggatca ccttcaaaac gaaagacatc tatattcaga atttgacctg atgccaaagc
     8941 ccgtaccggt atcgtggtgt gcgtgtagtt tttcatttta cttcagccaa ttttcagccg
     9001 gcacgctttc gcgcaccacg gcgtattgat tattttctat aatgatctcc gcaggaatat
     9061 cgtggcacaa aaagtacggc atactttccg taaagccgta tgcaccgcat tggcgaaaca
     9121 caagccaatc gccgcgttgt atatcttcag gcaaatcatg caaacccaga tcatccaagg
     9181 ccgtacataa ggggccgtga agtttgaaag attgggtttt cgcagacgaa ggcttcagag
     9241 tgtgtacagg aaacgaggca tgcgtgaggg ccggacgggc gatgtgattg acaccgcctt
     9301 ctaaaatcag aagctccgtg ccgcgtacct gcttacgatc catcacacgt gtaagataat
     9361 ttccgaaacc gcctacggca taacgtccga gttctaacca aatcgaaggt atttcatact
     9421 gcttgcgaat tttttgcaac gcgtcatcca cttcaatcca acgcaattcg cgcgcatcgg
     9481 catgatacgg tacgccgagt ccacccccaa ggtcaagcac ttccaaaggt ttgccgatat
     9541 gttgcatcaa ccgcttagat tcgacggcaa tacgatgcca aaaaaaattg agtttttcta
     9601 tgtcgccaat attgctccat tggaaaacat gacaacctaa cacatcgcaa tgaggaaacg
     9661 atttccatga caccgattgc caaacttccg gcggtaatcc aaacggggtg atggcgctgc
     9721 ctccaagcac gctggcgctc ttttcgtccc agtctaattg aaggcgaaga agaacctgaa
     9781 taggcttgtg ggtcgttgat gcaagctgct gcagcatttc aagttggtgc acgctctcta
     9841 cgatgatcgt acaaacgccg gcattcaacg cttccgtgac aaaggcttcg tctttggccg
     9901 ggccggtgag taatatgttt tttggtgcga tgcccgaata tcttaggatt tgccgtaatt
     9961 cgccgaggct ggcggcgtcc gccgaaaaag aacacgcacc cattgttcgc agaatggccg
    10021 aaagcggatt ggctttacaa gcgtaccaca ggcgaacgcc ttccgagcgt aacgtcatag
    10081 catgcgaccg taaaccatcc agatcgtagg caaaaaaagg ttttcgcact cggtcggcag
    10141 cctcttgtaa aagtgtaagg tgatgcgctg tcaatgcttt catcgcaacg cctcttccag
    10201 ttgtaacgcg gcgttgcttt tcgcatcgcg gtatttgacg atgatcgcac aagccgttgc
    10261 aagaccgcgt tctttggccc aggacgatga tagcgtgcgc gtaccgggta cgacgacggc
    10321 caaatcggga attctggcgc cacgtcccaa ttctttttca tgtacggcgt cgtacaccgt
    10381 gacaccgcgc gaaaggacca caccgggtgc gatcacggct ccggtgccga cacatatacc
    10441 ctctacgatc accgcgtccg cacccacaaa ggcatcgtct tcgatcacca cgggagcgct
    10501 gccgaccggc tccaatacgc cgccgatttg tacaccggcg ctcagatgta cacgttttcc
    10561 gacctgcgca cacgaaccga ccaacacatg actgtccacc atcgtttgtt cgtcaatgta
    10621 tgcgccgaca ttgatatacg acggcggcat gatcacaaca ccgggcgcca cataagcgcc
    10681 gcgtcgtacg gatgaaccgc cgggcacgag tcttattttt tgtttcggct gaaagcttcg
    10741 gggaatcatc gaatctttat ccacaaatcc gccatagcgt ccgtaggtgg gacgattcat
    10801 ggcgatcagc ttaccttcgc gaaaagcgga tagtatggct tctttgacgg caacattagc
    10861 ccgccaaaaa ccgttatcat acgtcgcggc ccgtatctct ccgcgctcca gttgttctaa
    10921 tatttttttc caatcgtttt tcatccgtat tgctccatcc atcgttgcac tagactgtcc
    10981 gccatgatta atttatttcg atccggtaaa tcttccatag aaagcggcgg tcgcacatac
    11041 gccgaagtga tcaaccccaa ttcgtgcaat aaagctttag caggtacagg attactggcc
    11101 aaaaacatcg catcggaagc cgtacgccat gtcggtaata atacgccggt atcgcggttc
    11161 acacaacggt aaacaaattc ggcggacgct tccggccaca cattgctcat cacagagacc
    11221 agccccacgg ctccggccgc tgcaaaaaaa ggtgttaagc cgtcgtcccc gctaaagaat
    11281 gcagcgccgg gtgcggcttc acggtaatcc tgaaattcgg taacgctccc gctcgcctct
    11341 ttcactgccc aaaatcgcga atgatcctta agttcacgca ggacggacgt atgcaacttc
    11401 acgcctgtcc gggatggcac attgtataac atacacggtt tttgagaggc ctcaagcagc
    11461 gcagcaaacc aactgcattg gccgcgtgca ccgggtttcg catagagcgg cgtgaccaat
    11521 aaatacgcat ggataaatgc gtgctgatta ttctcttcaa tccaccgctg ggtggcgtcc
    11581 aaattgaatc cgccgacgcc gaccataacc gggacatgga gtttgcaggt tccggtgaag
    11641 cggaccactt ctcttttttc atcatcgttt aacgccaggc cctcgcccgt acttccgagt
    11701 atcaccacac cgtttccggc ggcttcttga cgacgcaata accgttcaaa ggataaaaaa
    11761 tcaatttttc cgttatccaa aaacggcgtc acgatcgctg tccatagtgt aaccgattgc
    11821 agatttttca taacttattg tccttcgttt agccaggttt caaacacatc ttccagagga
    11881 aaaataccgc tgggataatc ctgcggccgc gatcgttttt cgatgagctt ttgggccgtc
    11941 catatcgcac cgcgcgcaaa tacggatcgg tcttcgacat gatgtgagag cataagtttt
    12001 tccgatgcgg tagccaacac aagcgaatga aatccgacaa cgtcacccgt acgatcgctg
    12061 tgaatgggta tttgttttcc gtaatgctgc cggatactga gcgcactgcc gctgggtttg
    12121 tcttttttat gtatgtgatg cacttcaaat atctccgctt ccgatccggc ccattggctt
    12181 tcaaggcgtc tgaattcgcg cgccatacga aacatccatt gcatacccat actaaaattg
    12241 ctcgccgtta tccaagtggc gccgctgttt tctacagcgc gccgcacgtc cattgtccat
    12301 tcaaatccgg tgctgccaca cacgacggga acggcggact gaatgagtac cggtaccaag
    12361 tcattcaaca catgacctgg cacaaaaacg atcgccacgt ccgaattttt caacgcctca
    12421 accgtcggtg gatttttgga attaaaaacc gccgcgattt catccggagg caataagcgt
    12481 tccacggcgc cgccggtttt cccgtttccg atcaggctga ttttaggcat acgacctcac
    12541 tttcgataaa cgcacggtga agcgcgctaa cagcttgtgg cgcatccgac tgatgcatca
    12601 gaaagcacat gttataagaa ctcgcaccct gacaaatcaa acgcacatta agaccggcga
    12661 tcgcgtcaaa ggtacgcgcc gcaataccgg gtgtttgttg cagattgttt ccgaccagtg
    12721 cgatcagcgt caggttttcc tcgatcgcga catccgcaaa ccggcgaaga tcccggagca
    12781 actcttgttt ttcggaaatt ttttcaccgg acgaacccag gcttgtgccg tctatcgtaa
    12841 gcgccacgct gacttcgctg gtcgtcacaa gatcaacgct gagtttatga tcggccagca
    12901 cgccgaacat tttggcaaga aaaccgtgcg tattgagcat gcgcaaactg ctcaccgtta
    12961 taagcgtctg atgcttgcgc atcgcgatgg cacgtaccaa cggcttatca ttgacatccg
    13021 gttcgatcca cgttccgccg gcttcgggtt caaatgtcga acccacaaac acatgaatgc
    13081 ctttgcgaat agctggccac aacgttgccg gatgtaaaac tttagcgcca aaattggcca
    13141 attcagcggc ttccgaaaaa ctgattttag gaatagtttg cgcagccggc accaaattag
    13201 gatccatcgt aaaaatcccc ggtacatccg tccaaatcaa aacttcttta gccgagagcg
    13261 cctctcccaa taatgccgcg ctataatcgc taccgccgcg ccccagtgtg gtcgtacggc
    13321 cgtcggtcgt tgctccgata aaaccttggg tcactataac ctgctcacca cacaaaggga
    13381 gcaatttttt caggcacgct gtttttaaaa gatccggttg cggctcggct ttgccaaaca
    13441 cctcatcggt aataataaat gatcgggcat cggcttccgt gacattattt ttgagatgaa
    13501 agagcatttt gccaaatata cgcgaggcca agcgctcacc gaatgaaagc catgcatcaa
    13561 gccatacttc gcgattttcc gatgcagaaa ataacgacat gtgattttgt gcggcaacga
    13621 tcaattcatc tactgcagaa gaatcaagtt ccagtgcatg gcacatcgtg cgatgccggt
    13681 cttcaatttt ttttaacgcc tgatctgccg acaccgtgtc gcatgcgcat agatcgtgat
    13741 atactttgag cagcgcattc gtcgtaccgg atgtggcgct caccacgacg acacgtatgc
    13801 ggttattggc aataacgata cgtgcgcttt ggcgcatcgc ttcggctgtg cccatactgg
    13861 taccgccaaa tttggcgata atgagatcgt tcatgactcc attcctcagg ttataaataa
    13921 aaaattcctt acaaacggta aggaagagtc atgatacggg aattgtacgg gattgagctt
    13981 aaggaaggcc ttaatgcaga tcgcgacgtc aattccggaa gcgcttcatg catcatgaca
    14041 gtcacgtggg tttaacctcc gcaaccaaca accgattttc cagatccgtt gtttcggcgt
    14101 ctatcaccgc cattcactgc tctagcgtgc cggatgcact cgcaatgaat ttttttgtag
    14161 acgctcctct tccgaaatat gtgatacatt ggtaagatag aacgaaaaaa aagaaatgca
    14221 acgggaaaaa cgatgtgccg aatttttaaa acgaattaac ctaaaacaaa aatctttttt
    14281 tgccgaacaa aaaagtttat gcataaaaaa aaggctgcac tacctgtgca gcctttgtat
    14341 atctacggcc tatgcgtata actaaaacgg taaatcatcg ctgcccgagg agcctcccgc
    14401 atcggcggca tgatccatac ctccgcccat atcggccgga tgcggaagcg acgcttcgcc
    14461 gcggcctccg tccatcttag cgcccagcat ttgcatgtta tcggcgcgaa tttccgttac
    14521 atattttttc tgaccgtctt tgtcgtcata cgaacgtgtc ttgatctttc cttccacgta
    14581 aacgagcgaa ccttttttaa gatattggcc gcaaatttcg gccaatttat tccacgccac
    14641 gatccgatgc caatccgtgg attcgatcag cttgccttct ttatctttgt aggattcgtt
    14701 ggtagcgaca ttaaacgtcg caaccggcgc accgctcggt gtatatcgca gttccggatc
    14761 accgcccaga cggccgatga tgatgacttt attgagtgtt ccttttgaca taagtatatc
    14821 cttactcttc tatcgtttta ttttgatttt ttttcggatg cggtcggcgc aggcttcggc
    14881 gattcttttt ccgtcgccgc cggtgcgata cccaattcct gacgcacgcg ggtttcgatt
    14941 tttttcaaga cgaccggatt atcggtcaga tattgtttcg cattctcgcg gccttggccg
    15001 attttttcat cttcaaaaga aaaccatgtt ccgctttttt ggacgatatt catcgtggac
    15061 gccaggtcca ccaaatcgcc ttcgcgcgaa atgccggtac cgtacatcag atccacttcg
    15121 acttctttga aaggcggcgc cagtttattt ttcaccactt tgactttggt gcggttaccg
    15181 acaagatttt caccggcttt gatttgtccg atgcgacgga tgtccacacg aatggatgta
    15241 taaaacttca gggcccgtcc gccggtcgtc gtttcgggat tgccgaacat gacgccgatc
    15301 ttatcgcgca cctgattgat aaaaataacg caggttttgg atttgctcac cgcgccggtc
    15361 agtttacgca tggcctggct catgaggcgt gcctgcacac ccatggtcgc atcgcccatt
    15421 tcgccgtcaa tttcactgcg gggaacgagg gcggcaacgg agtcaatcac gatcacatcg
    15481 acggcattgc tgcgaaccag acgttcggta atttccagcg cttgttcgcc gttatcgggc
    15541 tgagaaacta aaagattatc aatatccacg ccaaggcgct tggcatatat cgcatcgagt
    15601 gcgtgctcgg cgtcgataaa tgctgccacg ccgccgactt tttgcgcttc ggctatgaca
    15661 tgcagcgaca gagtggtttt accggaagat tccggcccgt aaatttccgt aatacgtccg
    15721 cggggaatac cgccgacgcc cagtgcggca tcgatcgaaa tggaaccggt ggaaaacacc
    15781 tcgacctgca tggccgtgcg ctcgcccagt ttcatgatag atcccttacc gtattctttg
    15841 acaatgtctg tcagcgtgcg gtccaacgcg gcttttttgt ctttgtccga caataccatt
    15901 tcgtctgcca tcctaagctc cttgtttcgt gtgtgtctgt agttgtgctt tgttatttac
    15961 gtgcgcaaag tataaaaacg cttttttacg tgcaaataaa attccgtgca aaacatcgta
    16021 ctgtcaaagc ttgccggttc atggataccg acaagctctg gcgtgcacaa aacggatgtc
    16081 atacggtggc ttcggcaaga atttccattt cttcctgtac accttccgac tccgacgact
    16141 tggcgttctt atgccccagc atctgcatcg tttcggcgac gacttccgtc aacgcatgtt
    16201 tacccccgct cttgtcatcc caccgacgtg ttttcatcgg accctccacg cataccagag
    16261 accctttttt caaatactgg ccgcacacct ccgccagacg gctccacgcc accacgcgat
    16321 gccaatccgt attctgcacc agtttcccct ctttgttttt aaatacctgc gtcgtcgcta
    16381 cgctaaacga cgtgatcggc gtccccgtag gcgtatagcg tacctgcgga tcacggccca
    16441 gacgaccgat caatacggcg cgattcagac ttcctttgtt catgacttgt tctccttgtt
    16501 ttgtttttat gtgttattag taaattcttt ttcgtttcat tttttgtatc atttccatca
    16561 tcgtaacatg atcaatttat agtcagctga cggccctttt ctgtcaattt ttgacaatat
    16621 ttaccccatc cctgcatttt ttgattttgc cgtttatcac cgcacgttta gcattttggg
    16681 gtactaaata ttcacaagcc gctccggtta atcctctatg ctctgagccg atacatcctg
    16741 taacctttac tccggctttg cgtatctttt ttcgattttt atgatagacc gctaatcctc
    16801 gtaatacgga ttgatttatt actaaaacct gttaattttt atgcgttgta agcatactga
    16861 atacagaaag gcacccttta ccatgttacg ttcatgtttt tttattgcga tagtctgcac
    16921 ggttatgtat ctgggttgtg caccccaaaa cgtcgaaaca ctccccgatc cgctggttca
    16981 taaccgcgac agttcggtca cccccggcca agattttttc ctgcatgcca atggcggctg
    17041 gttcaaacgc aaccctattc ctgagtcgga atcctccaac ggattgtggc tgacgatcgg
    17101 cgaagagaca cgcgccgccg tacgggagat ctgcgaaaaa tccgcacaag aaaccaaagc
    17161 gcccaaaggc agcaataaac agaaaatcgg tgatttttac gccagtggta tggatagcgc
    17221 cgcatcggaa aaagccggta tctcgccctt aaaatcggaa ctggatcgca ttgacgccat
    17281 gaagtccatc agcgaattag ccgccgtagc cgcacgcctg catcagatca acggcgatcc
    17341 gattttttca tttaatatct acaaagacga taaaaaaccg accgaatacg ccgtcgcgct
    17401 caatcaaggc ggactggggt tgccggatcg tgactattat ttcaaaaacg acgaacgcac
    17461 caccggcatt cgtgcagcgt atcaaaaaca tttgtttcgc aattttgaga tcgtcggaga
    17521 atcgcccgac caagcgcgcg ctttcgccga tcatattttc gctttggaaa aaaacttagc
    17581 caaagccagc cgcacgctcg aaggcctgcg cgatccgtat gccaactata acaaaatgag
    17641 tgtcgccgaa ctttccaaac tcaccccgtc gtttgactgg aatgtctttt tccaaaacac
    17701 cggcctgaaa aacgtagatt ccgtgatcgt aggacaaccg gaatttttta aagaagtgga
    17761 aaacaccgtc aaaaatactt ctctggatgc atggaaagcc tatctgcggt ggagtgtgat
    17821 cggcacgtac gccgcttacc tcaatcaagc gctggtggac gaaaatttta atttttattc
    17881 caagaccctg tacggcgtca aacaaatgaa accgcgctgg aaacgcgtcg tcgaacgcac
    17941 caatcgcgct ttgggcgaag tcatcggcca ggtctatgta gaggaatatc tgcccaaggg
    18001 taccaaagaa aaattactcg aaatcggaaa caatatccgc gacgtatgcc gcgaacgtat
    18061 cgaaaaagta agctggatga gcgacgcgac acgtgagcgc gctttgctca aactgcaaac
    18121 tatggcgatg aaagtcggac accccgacca atggcgcgac tacagccgtc tgtccgtcga
    18181 acgggacacc tatgttgcca atgtgatgcg cgccaatgtg tatgaatttg atttcatgat
    18241 cggcaaatac ggtaaacccg tagatcgcac ggagtggaat atcaccccgc aaacgtataa
    18301 tgcgtattac gatccgacca ataacgaaat cgtggtaccc gccgccaata tcatcgtccc
    18361 gggttttggt aaaacgatgc ccgacgatgc gatcttatac ggtattatcg gcggcagcac
    18421 gtttggtcat gagatcatac acggcttcga tgatcagggc agccaatacg acgaaacggg
    18481 caaactgaat aactggtgga ccaaagaaga ccgcgcccgt tttgaagcca aaaccaaaat
    18541 gctggttacg cagtttaata actacgtggt actcgacagc gtgcacctgc gcggcgaagc
    18601 cacgctgggc gaaaacatcg ccgatctcgg cggcgtggaa ctgggctacg atgctttcaa
    18661 acgctccgcc caatacaaaa acaatcaaaa agtagccggt ctgacgcccg acgaaaggta
    18721 tttcctcgcg tacgcgtacg catggatgat ccagatgcgc ccggaaatgc tcgccagccg
    18781 catcatgtcc gacgtacacg caccggcaca acaccgcgtc atcggccccc tttccaataa
    18841 tgtcaagttt tacgaagttt ttggtatcaa gcccggcgac gcgatgtaca gacccgaaaa
    18901 cgaacgggtc agcatctggt agatcagcgg tgttttaaat ttttttatca aatggaaacg
    18961 tgtttatcgg gttggctcac cgatgaaaga gagtccatcc gtcttgagca aagtcgaaga
    19021 cggatggcac taaaccgggc catggctccc cccccccacg caccatgacg tatgggagtt
    19081 ttcccgataa gcacaaataa aaataaatat agccaatata aaaaattggc tatatatttt
    19141 tttttacagg gtcagtggag cgtggcacgg gcagataatc gctgtacgtc agggtcgata
    19201 tgtagccagt cataagtcac agtatgagga aacatgcgtt tttttacttt atttttttct
    19261 ctcaatcgaa agcccgacac acaagccaac gtttagcaaa accaaaagag ccacttttta
    19321 gccaacgcgc cgacataaat attaactttg aaacaaaaga aaataggtaa atgaagttat
    19381 cagacccgtt taaaatattg acaccgaatg aaaggtgggc gcctacacaa agccaaatgg
    19441 acgcttttca aaatgcctat gaaaagttgt tgccaccttt ggtttataaa atcagacttg
    19501 cggttgccag gtggagagac gaaggctatc aaggggcttc gaatacttca aaatcattat
    19561 taaacttttg gtttaatcag gaacatctta tcgggcagtc aaaatttagt tttttctttg
    19621 cacaacgaga agccattgaa tcaattattt acttgtatga aatagccagg gcaaaagaca
    19681 aatacgagtt aatgcgattt gactcttccc aacgtgtcag cactggtatg tttgatgaaa
    19741 actggacacg ttatgtaatt aaaatggcta cgggagcagg taaaacaaaa gtaatgggta
    19801 tgactttggt ttggtcttac tttcataaac tttacgaagc ggacagcaca ctatcaaaag
    19861 attttctagt tattgcaccc aacatcatcg tattaaaccg cttacgtaaa gactttgacg
    19921 gacttaaaat gttttttgat gagccgtttt tccctgacaa tggctacgac gataaagact
    19981 ggaaaaacga ttttcaatta acactgcaca ttcaagacga tttaaaacca ataacggaat
    20041 ctggcaatat tttccttacc aacattcatc gtgttttctt taacgaagca cctgaaccaa
    20101 attttgaaac gactttttta ggcgttaaac caaaacccga tgcagacact tcaaaagggt
    20161 tagatcttgg taaagtattg cgaagcgaca agattaaagg cttggttgtt ttgaatgacg
    20221 aagcccatca tatacacgat aactcattgg catggttcaa aagcattgaa gacatcagta
    20281 acaaattgaa attgaaaaac ggtaatggaa taagcctgca ggcagattat tctgctactc
    20341 ctaagcataa caatggtgct atttttgttc aaaccatttg cgattatcct ttggttgaag
    20401 ctatcaagca aaaagttgta aagtcgcctg tattgcctga cgaagcatca agacaaaaaa
    20461 ttcaggaaaa agattcttcc gactttgtag aacgctaccg agattatatg cacttaggtt
    20521 atttagagtg ggaacagcaa tatgaagaac taaaaagcca aaaaactccg atacttttca
    20581 ttatgacaat gaacaccaaa gaagcagacc aagcggctgc ctttttggag gcgagttatc
    20641 ctaaaatgaa aaattcagtt ttgacaattc acaccaacaa ttcgggcgaa ataaaagaaa
    20701 ctgccacaag caaaaaagat aaagaagaat tagaagcatt acgaaaagca gcagacgaaa
    20761 tagacaaaga ccattcgcct tacaaagcag ttgtctcagt tttaatgctt cgcgaaggtt
    20821 gggatgttaa aaatgttacc accattgttg ggcttcgccc atttggtgcg gattctaaaa
    20881 tccttcccga acaaactatt ggaagaggtt tgcgaaaaat gttttcgttg gatatgcccg
    20941 aaaaattagt tgtagttgga acaccagcgt ttttagagtt tgtcgagagc ctgaaaacag
    21001 aaggtgtaga attccaatac agcccaatgg gcaaaggctc taaaggcaaa tcgcctgtaa
    21061 ttgtggaagt cgataaagaa aatccaaaca aagatttaga tgttttggat attcctatcc
    21121 cgcaaatgag tccaagaatt tacagagagt ttaaaaacct tgaattaatt gatgttagca
    21181 aatttaaaaa cgaaaaagtc attcagaaga aatttaacag cgatgaatta aaggaaattg
    21241 tattccatga tatagatggt gatttttcac acaaaaccat ttataaggaa acaacccctg
    21301 attatagaaa tgtgattagt ttttttactt ccacaatttt aaaggacagt cgcttggtaa
    21361 gcggttttaa tattttatat cccaaagtcg aaagctttat aaaataccag ctctttacaa
    21421 aagaagtgga attaagtaat ccgcaaacga tgaggaacct ttcagaaata caaccaaagg
    21481 aaattttgaa aacaacgttc aaaaaagcca ttgacgattt gacgataact gataaaggaa
    21541 cagcggaggt taaaaacttt atttcactta aactaacaaa accgaaggtt gcagaaaacc
    21601 aaccattcct tgtaccaaag aaatcggtct ttaataagat tattggagaa aacccttttg
    21661 aattggaatt ttctgctttt tgcgaaaaca ggtttaatga tgttgtttca tttgctaaaa
    21721 atacgatggg tgaaggcggt atcaatttca aaattgagta tcaagcaact gatggaaata
    21781 ttcgcgagta ttttcctgac tttttcgtga agattgataa ccgtactttt tttgttgttg
    21841 aaacaaaagg acgccaggat ttggatgata ttttaaaaat caagcgattg gcttcatggt
    21901 gtcatgatat taataacgct caaaaagagt atacctacac ccctgtttat ataaagcagg
    21961 aaaagtggga tgatattaag gacaatctta aatcatttca ggatttaaaa accattttcg
    22021 aggttaaatg aaaccctggg ttactaaagc gttacattat ttagagcaga gtctgggcga
    22081 aattcctcag gaactaaacg agcttgactg gaaagaagat ttatcaccta acaatgaaaa
    22141 actttccaga catttgagtg cgtttgccaa tcaccccggc ggtggattcc ttgtatttgg
    22201 catagataat atttctgcga aacctgttgg cctaacaaga gaatccgtaa gcattattct
    22261 tgatagactt tccaatcttt gccgaaatgg tgttgatccg gtcgttgtga tcgatcatat
    22321 cgtccatccg tttcgtgatg tacaacttct tattattcac ataaaagaaa gtgccgtcaa
    22381 gccagtccac atcgcaaata aaacgatgga agaaagctat atcagaaccg gttcatcaac
    22441 ccgaaaagca tccagacagg aaatcggtgg tttgatgtta aacagcaaaa cacccgtttt
    22501 tgaggaactc catgcttcaa aacttaaaaa tgctgtcgaa attatgacgg ctttagattt
    22561 tgcagccatt tataaactac tgaaaaagcc cgttccaaca aacctcgcag aaattatccg
    22621 ttggcttcag gatgaaaaaa tgattgttca aattgaagat gacggatttt atatcaccaa
    22681 ttttggtgcg ctttcagccg ctcaaaactt aaacgatttc gataacctgt cccgaaaatc
    22741 gatccgcctt atcaaatatg agggaaataa taaaacggtt caaagcaaag aatttcccgg
    22801 ttcgaagggt tatgcgatcg gttttgaagg gctgattcaa tttattaaag gtattctgcc
    22861 cggtagtgaa atcatcaaaa atgcactgag agctgaaacc tctatttttc ctgaaatcgc
    22921 acttagagag ctcatagcca atgcactcat tcatcaggat ttttctatca gaggttccgg
    22981 gcctatggtt gaaatttatg acgatagagt cgaaatcacc aacccgggta aattgctgcc
    23041 taacaaaaat attgaccggc tgattcggac aacacctgaa tcacgaaatg aaattttggc
    23101 tgccgctttc agacgatata atatctgtga agaacggggt tcaggttttg aaaaagcagt
    23161 caccgccatc gaactttttg gtttaccgcc tttaaggttt gaagaaactg acgggtcatt
    23221 taaagtcgtc atgtatgctc cgaaaccttt cgctgacatg tcggacaagg agcgaattga
    23281 ggcgtgttac cagcattgta ttattcaata ctacggacaa ggcggcatga gtaattcatc
    23341 gttacgaaaa cggtttggaa tgcatgacaa acaggcatca caagtttcac ggttgattaa
    23401 actggcgata gaaacaggca gaattaaata taaagatccg aataaccagt cgacaaaatt
    23461 aaatgagtat attccgtatt gggcttaact tgcttgattg ggttagggag ttaatttgta
    23521 agttattgtt aatcaataat atgctgtttg actgagattc ggaaaggctc aaaacagata
    23581 ccaaaaaact aagtcttgac ggtgataacg gtcctgtttt ataccaattt tcaattatta
    23641 ctgaccgaaa ccattttgtt tgactgagaa tcggaaaatt ggattaaacg attgaatatc
    23701 aatagtatac ttcttttctg atcaatttat aagataggtt acaccacatt aaaccagcga
    23761 aaaaatacag atgaacgata ccgataaaaa caaaattatt gaactcataa aggcaggaga
    23821 aaaactgcct aaagaattta tttataaact ttttgctgac gaagaagatg tctttctttt
    23881 ttggaacgga agaaaagaag atgtaaccaa cattgccctg cctttccata gcattgaaca
    23941 tattgatgag ccacggagag aaaaagacaa ggcacaagcc agtatgtttg aaaccgattt
    24001 tagaggcaga cagctcaaag gttggacgaa taaactcatt tggggcgata acaaacttat
    24061 actttcctca ttggcaaatg gcccaatgcg ggaagaaatc gaaaaagaag gcggactaaa
    24121 actcatttac attgacccgc cttttgccgt tggtgccgac tttggtttta atatagaaat
    24181 aggtggcgaa acagccgaaa aaaagcaaag tattattgaa gagattgcat accgtgatac
    24241 ttggggtaaa ggcatttcgt cttatctgag tatgatgtat gaacgattaa agctgatgca
    24301 taatttatta gcagatgatg gaagcatcta tgttcattgt gatggaagag tgaatagtta
    24361 tttaagactt attttgcagg atgtttttgg tactgacaat aatttgaatg aaataatttg
    24421 gcaaagaact acagctggta aattcatctt aaataatcta cctaaaaact ctgattatat
    24481 tttgtggttt tctaaatcgt caaaatacaa ttatacgcca atcacatcgg aactatctga
    24541 agataataag aagacgtata tacatgatga tgaagatggt aaaggtccat ataatacaca
    24601 accattaatt aatcctaaag attccgatag acctaactta agatataatt atactgactt
    24661 atcaggtaaa gtatggtctc atccgccaag tggttggaga gtaagtttgg aaaaaatggt
    24721 tcagcttgat aaagaaggta gattgtattt tacaggaaaa accttgagag aaaagaatta
    24781 cctaaatgaa agagaagaaa aggggaagca aatttctaat atgtggacag acattaatgg
    24841 aaattcaatt cgatacagtg atgaatctgt agattacccc acccaaaaac cagaagctct
    24901 tttggaacgc atcatcaaag cgagtagtaa tgagggagat ttaatagcgg actttttttg
    24961 tggaagtgga acaacagcag cggttgcaga aaaattgggg cgtaagtgga taaccactga
    25021 tttgggaagg ttttcagttc acaccgcaag aaagcgttta attggtgtgc aaagagagtt
    25081 acaagaaagc ggtaaagatt tcagggcttt tgaattgctg aaccttggta aatacgaacg
    25141 ccagtttttt atggacgacc ttaccaacgg taaacgcaaa gccaaagaag atttatatgt
    25201 tgatttaatt ttagaagcct acaaagccaa acgaatagac ggtcatgcac atttacatgg
    25261 tcacaaagcc ggtcggtttg taaatgttgg ccctttagat gtgccggtaa cacaaagccg
    25321 tttgatggat atttttgaag aatgccgcac caaactttac acccaagttg atgtgttagg
    25381 ttttgaattt gaaatggggt taacgccgca attcattcag gaattgaaag agaaaggcgt
    25441 ttctgtaact ttgaaataca ttccaaaaga tgtgtttgat aaacgagccg ttgaaaaagg
    25501 tcaagtgaaa ttctttgatg tggcttactt aaataccaaa gaaaaaataa aaggtaaatc
    25561 tataacaatt gaactcacgg attttgttac acattacacc caggatgaca ttgaagaact
    25621 gcaacaatcc atgcgagcag gaagcacagt tgtcattgaa gacgggcaaa ttttcaaaat
    25681 tgaaaaagaa aaaaacggaa ttgttacccg aactgttcta acaaaaaact ggttcgactg
    25741 gattgattat tgggcaattg actttaacta cgaagacaaa aaggaaatca tcaaaatcaa
    25801 aaacgaaaag ggcgaaattg aggaaaaatg gagtggcaac tatttgtttg aaaacgaatg
    25861 gcaaagtttc cgcaccaaga aaaaccctac actcgaattt acaagcatag cccacgaata
    25921 caaatcagca ggtaaatata aagtaatggt caaagtggtg gatattttgg gagtggacac
    25981 gagtaaaata attgaagtaa gtataaaata actatagcaa acgatgttca caaagtcttc
    26041 gtcagctatc atcacacaat gagcgggcaa taaaaaaaca atcaaaaagt agtcggcctg
    26101 acgtacatgc accgtcacag cagcgcgtca tcggcccctt tccaataatg tcaagtttta
    26161 tgaagctttt ggtatcaagc ccggcgacgc gatgtacaga cccgaaaacg aacgcgtaaa
    26221 tattttgtag atcggcggta tttactgtta atacgtaaaa aaaaatcttt ggtgccatgc
    26281 cggggatttt ttgtgaaagg aaataagtaa agggatgaca tgacttagaa tggcatttcc
    26341 aaaggtgact gctgggtaac agttttgtgc ttagaatctc gcccgaacgc aacccccaca
    26401 aaccgtttac aatccactcc accatccacc actccgtcaa gccggaaaac gttggctgct
    26461 atatattcgg acgcctcaca atttttgata accaagaaat aaatcaatga tattgaaaaa
    26521 agcatttctt ctactcatta cagcttctct tgcgacattt ttgcaggcac aacagctatt
    26581 gacaaaagac cagcaagaag ctcagcaaac cgttgtaaaa atgtttgaag ctttatcaaa
    26641 acgagattcg gtaaatctta aagcccattg cactgccgac attactttgt atgaatacgg
    26701 acaggtttgg aatattgata ccttgaccca taaagcaatc accaaaaata ccgatcccga
    26761 ttttaatcgt accaatactt ttgaattcat caacaccgaa acagataccc atacagcatg
    26821 ggtaacatac cgactcaagt caacaatcac caaagacagc aaacaaacta ccatacaatg
    26881 gcttgaatca gtatttttag aaaaacaaaa aaatcaatgg aaggtaaagc atctccattc
    26941 tactctaatg agtcgaaatt aaagattcaa ccccgcaatc ccccactccg gcaagccgca
    27001 aaccgtttct aaggcctatg atttcgtcag cctcctacta gggccgctct attcttttga
    27061 aaattcgatt gacaaatggg gttttagttc ccacattata cgactatttg taacccctca
    27121 cggcttattt tccatgaaaa aaatcagaag ctaactcaag gcgatgaggt tgtcccatca
    27181 tgctactgga tgtaaactaa caatgaaaaa acaacgatga aaacctatcc cagtattttc
    27241 aacgatgtca tcggtccggt catgcgagga ccatccagtt cgcattgtgc cgcgtcgctg
    27301 cggataggcc gcataggccg tgacctgatg gacggtgata tccgggaagt gtatatcgaa
    27361 tttgatcccg acggctcgct cgcaaccaca cacaaaggcc aaggttcgga tatggggttg
    27421 tttggcggat ttttgggatg ggaagcctat gaggaacgcc tacccgacta tcagcaggcc
    27481 gtagatgaag ccggcataga cataaaaatc gctattcatc ccatcggcgc tacccacccc
    27541 aatacataca aaataacctt aagcaatgcg tctgaaacaa gggaactcac cgccatctct
    27601 accggcggcg gaatgatcga agtcatagaa atcgatggcg cgcaagtccg catggccgga
    27661 gacttttatc aaacgctggt ttacgtgaag tcgcccggcg agattatttc gttcatcgaa
    27721 aaatcgtttc catgcgatga gatcggattg cgaaaaggaa aatcaacttt catcgaaatc
    27781 aaatccaatc agtttctccc cgatccactc tgtcgcgaat tgcaatccat agacgatgtg
    27841 ctttttataa aaaaattgaa cccggtcctg ccggtgatgt cacgaaaaga cttaaccgtt
    27901 ccttttatca catgcgaaga aatgctggca tataacaaag gaaaaaacct ggaattgtgg
    27961 gaactggccg ttgactatga aagcatgcgc ggtaatatca cgaggcagga agtttttgaa
    28021 aaaatgcgtg ccatcgtaga gatcatgcaa aacgccattg ctcttggcct aaaaggaacg
    28081 acctacggcg atcgtatatt aggcgcgcaa tctgtagaat ttcaacagca gatgaaggac
    28141 aagaagttga tcgaaggcga tgtactaaac cgcgtgatac tctatacgtc ggccatgatg
    28201 gaagtgaaaa gttcgatggg cgtggtcgtg gcggccccta cggcgggatc gtgcggcgca
    28261 ttacccgggg ctgttatcgg tttgagcgat gcgctccatt tttcgcaaga cgagatggta
    28321 aaagcgatgc tggcggccgg tatcataggc gtatttattg cggcgcatgc tacgtttgcg
    28381 gccgaggtgg gcggctgtat ggccgaatgc ggatccggat caggcatggc ggcggccggt
    28441 atcgtgggaa taaaaaaagg cacgctcgat caggcgctgg cagcttcttc gatggcattg
    28501 cagtcgtctt taggcatgat ttgcgacatg atcgccgacc gcgtagaggc gccctgcttg
    28561 aatagaaacg tcatggccgc aaccaacgca ttgtcctgcg ccaatatggc cttgtccaac
    28621 tataatcacc ttattccatt ggacgaagtg attgaaacca tgaaaaaggt gggcgacgcc
    28681 atacccaata cattgcggtg taccggactg ggcggcctgg ctatcaccaa aactgccaaa
    28741 aaaatagaag cgacgctcga aaagaaccaa gcggagagca acaagaagtt ttttaaggtg
    28801 tgttaatcca cccgtttttt ttcatccgca tatcttttcg gtacgacgaa attgtactct
    28861 acgatttccc attattaata atgaaacatt gaaagcgcat actatggaca atgccaaaac
    28921 caaaaaacta aaaaacggag acccctgtat tgtcatcggc ggcacacata agggaaaagc
    28981 cggcattgtg caagacctga ataccagtaa aacgggtcac attactatca ctgttttgca
    29041 aagtaatggc gttagattta agactcttgg cagaaatgtg gaagtcgtaa agagcaaaaa
    29101 aaacgccccg taacacgggt tttgtttcag gcaagtggca cgcctgtaca tgcaataaaa
    29161 ctaccctgcc ctcgccaaac ccgaaaacgt tagatggtaa ggcgtgtata ttgcatccgt
    29221 aatttttttc atttcacatg gaacagtttt tttatacttt tacttattgt ttttaacaaa
    29281 cgggataccg tgtataaatg agtaagttag agaaaatttt attaactgtt ttacgcggta
    29341 catcggatac gaatatagac tttgaagact tacggaaact cttacataca ttaggatttt
    29401 ctgagcgaat aaaagggagt catcacatat tttacaaaga cggtgtggtt gaaattatta
    29461 atcttcagtc taaaaattca aaagcaaagc cataccaagt taagcaggtt cgaaatttga
    29521 ttattaaata caagcttgga ggagacgaaa atggataaat atgaaattat tttgttttgg
    29581 agtgaagagg ataattccta tgtcgcagaa gttcctgaac ttcccggatg tgccgcagac
    29641 ggggcaacat atcacgaagc actaacgaac cttgaggttt ctattaaaga atggattgaa
    29701 acggctaaag tgttgggtag ggatattcca aaaccaaaag gtcgtttaat gtacgcctga
    29761 aaacgtcctc attgccctgc atcacatagt gatacggtaa tcccctaaca cgatactcgt
    29821 agaatatgaa aaatagagtt ttttatagat aatgaaggat tgctaataat ataatccggt
    29881 cttgcaccgt gccgatgcct cggtcgtcgt acttcgacac attacaaact aacttaccta
    29941 aaaaacacga cccctgaaaa tttttggctt caacgttaaa aagttaaatc aactgaaaga
    30001 gttaccggta tggcgagtat caatccccac atcaatttca acggcaatgc ggaagaagct
    30061 ttcaattttt acaaatccgt atttggcggt gcgttcgcaa aaataattcg cttcaaagat
    30121 ttggccagcg ctgactttcc ggtcgccgaa catgaggcca ataaaataat gcacatcgct
    30181 ttaccgatcg gtaaaagtat attaatggcc aatgatgtac ccgaaatatt gggacggact
    30241 aacgaaaatg agaaccgaag caaaattgta gtcagcgcag aaagcaaaga agaagccgac
    30301 aagatattcc atgggctttc ggcgggtgga caaattgaaa tgcctatttc tgacagccct
    30361 tggggttcct attttggtat gtttagagac aaatatggaa tcgagtggat ggtagatttt
    30421 gatgcaagat acaagggaca gatatagccg ataaccttga cagcatctac aaaaaataat
    30481 cactcaatga ttgaatcatt gaattacaag gcaacttgaa taaagttaat atacaaacgc
    30541 gtattatgag cgctgccaaa atatccatac gtttttttaa tgaccgtgaa gtgcgagccg
    30601 tatgggacga acaaaatgct aagtggtggt tttcagtgtt agatattgta gcggtactta
    30661 ccgatcaaga tgattatacc aaagctcgta actactggaa atacctgaaa gccaagctca
    30721 aaaaagagcg tagtcaagtg gttagtgcca ctacccagtt gaaattactg gcgccggatg
    30781 gcaaaaaacg catgtctgac atgcttgact ataacggtat catagcctta ggaaaaactt
    30841 ttcccggaac aagagccaac cggtttatcg aatggtttac ctacagtaat gaaagtatag
    30901 acggaaaaag taaaaccaag gcctatgccc tttttgaaag ctctttcatc aacagcatag
    30961 aagtaggcac caccaaggga ttgcaacaga tacatgctta tctgtttggc ggcttgtatg
    31021 aattcgcagg acaaatccgg aaaaaaaaca tttcaaaaag cggtttccga tttgccgttt
    31081 caggtttttt aggtgaaact ttaaaacaaa tagaaaaaat gcccgagact acttttgacg
    31141 aaatagtaaa taaatatata gaaatgaaca ttgcacaccc tttcatggaa ggcaatggcc
    31201 gtagcgcccg aatctggctg gatttgattt taaaaaagcg tctcaaaaaa tgtgtagatt
    31261 ggagccaaat aagtaaacgc gactatatga acgcaatgat gcaaagtccg acaaaaaacg
    31321 atgcgcttaa aaagctttta aaaaaagcac tgacaaccaa aatcaatgat cgggaaatgt
    31381 ttatgaaagg tattgactat tcgtattact acgaagaaaa cgattaaaag aaaattgcct
    31441 tcaaccatca caaactttgc tcttaaaata taccccgcgc ttgttgtacc ctctccacgc
    31501 cactgacttt tcgccgcatt cctcgtcttg tttaccgggc gcgatgtata gctcccaatg
    31561 attaaaaatt gttggttttc taacgacaat catttacttt tgtcgtataa tctcatttat
    31621 cgcatactct tctttctgat tgtaatcacc tgttcggagc ctgcgccctc cggttaattt
    31681 taggcgttga ttgatttgac gacggcattt taaaaaaacg gcttttgata gcatagtttt
    31741 gtaaaaatga atcggaggaa aatcatgccc ctgcgttaca tgcttgcgtt tttttgtgtg
    31801 agcgcccttc tcttcggcca agacaaaaaa ccctccatcg ccgtgctgga ttttaaagca
    31861 tccgaaggat tgacccgcgg agagaccgct acgctgacta accgttttcg gggaatactc
    31921 gtacagaccg aagcctttaa tgtcgtcgag caggaaaaaa tgaatgaaat cctgaaagct
    31981 caggatttta gcatgtccga tgcctgcaat accgccgaat gcgccgtaca agtcgggcaa
    32041 ctgctcggtg tcgaacgcat gatcgccggc gacatcggca aaatcggcga cgtcttcacg
    32101 attgatttac ggttgattga tgtttcgacg ggaaaaatcg taaaaaccgt cacacgcgat
    32161 tacgaaggaa aaatttccgg tttgctcggc gtgatgaaag atgtggcgag tacatttgcc
    32221 gcgatcaaac cggtttcccc cgccgtcggc tccatcacca tccagtccac accgtccaaa
    32281 gcgacggtct ccatggacgg aaaagaatta aacttcgtga cgccttttgt agccgaaaac
    32341 ctgacgttgg gcgttcatga gatcgcactg cgtttaggga cgatgaaagc cgtcaaaacc
    32401 atagagatca aagaaggaaa aaatccggta ttaaatctac gattggagcc gatcacggcg
    32461 ccgctctccg tcgcttccga tcccgccgga gcgaccgttt ttgtcaatgg cgatcgctac
    32521 ggagacacgc ctttgcaaat aaagttacct atcggatcgc atacgctcac cgtcaaacaa
    32581 aaagagtacg aagtgttcac ccaaagtatc gttctcaaag aaaatgatcc gcctttggtc
    32641 attcaggctt cactcacaaa aaaaataaaa actctgtccg aatcctcttc caacaaaaaa
    32701 tggtggtaca tactgggcgg ggcagccgta ttgggcggcg gtaccgcgtg gctcttgagc
    32761 ggcggcggca aaagttccgg aaaatcaggc attcctccgg ccgagtttcc gaattaatag
    32821 ttttgcaaaa catcatttaa aaatacttaa ggcaatctgt atgcgctata cgataaccat
    32881 tgcagccgtt ttagcccgcg tgtttttttg tgtgacactc ctcccgtcat ttattttttc
    32941 tcaaggcgga gcttggtcgt ctaaaacgtc catgaccacg gcacgttggc attccggcat
    33001 cggcgtgatc aataatacgc tctatgtggc cggcggctac aacggttcgc atcagacggt
    33061 cgtcgaagcg tataatttta cgagcaatac gtggaccaca aaaaatacgc gcccggtaca
    33121 aacatccgcc gcgtacggtg tgatcggtaa tacgctctac acggccggcg gcacgaacgt
    33181 gagtgtacgc atatcggacg tgtgggctta cgatccgggg ctcgattcgt ggacgacgcg
    33241 tgcaccgatc cccaacctgc gctctaatcc cggaagcgcg gtggtgaata acatgctata
    33301 cgccatcggc ggcgtagata acagctctgt actttcctct atggatgcgt atgatccttc
    33361 cggaaatgca tgggcatcca aagccgctat gagcgttccg cggcaagccc cggcggtcgg
    33421 cgtgatccaa ggcatcatct atgtggccgg cggtttcacg acgggaaatg ccgtaaccga
    33481 tgtgctggaa gcatacaatc cggtaaccaa tacatggtcc acaaaagccg tactgcctac
    33541 ggcgcgcgga tatgccgtcg cggcggtact cgatgacaaa ctttacgtca tgggcggtgt
    33601 caccgtaggt ggcggaagcg ctattcagac cgtcgaagtt tacgatccgg taaccgacac
    33661 ctggtcgggc ggcacaccca tgaaaaccgg acgcgcagga atggaagctt ttacctatga
    33721 taacaaaatt tacgccatcg gcggatggtc cggcgtcagt accgcttcat ccatattgga
    33781 agcgttcaac ggccgaccgg tgattttttc atttactccg gcattcagcc ccgtggattc
    33841 gatcgttacg atcacgggaa ataatttcag cgccgtaccg caggaaaatg tcgtgtattt
    33901 tggctcggta aaagccaacg tcaatacggc ttcgatcacc caattgacgg ttaccgtgcc
    33961 ggccggacaa aacttcaaac cgatcagcgt cacggtgggc ggttatacgg cgtttgcaaa
    34021 aaaatctttt tttgtcacga aaaaagtaaa aagtacgata gatgaatctt catttgatac
    34081 gcggattgaa ttgacgtcag gaaataatcc taaaaatgta gcctccggtg atattgacgg
    34141 cgacggtaaa ccggatctga tcatttccaa ttttgatgac aatacgattt ccgtatatcg
    34201 aaatatatcc tcgacgggaa ctctcagcgc aggatcattt tctggaccgg tagattttaa
    34261 tacatcatta agcccgcatg gcatgaaaat cgccgatatg gacggcgacg gaaaattaga
    34321 cgtcatccta tcgcacagca gcagcagttt tgttaccgtc cttcgcaata ccgcgacaag
    34381 cggcgttatc aatgcttcat cgtttgcttc agcgataagt tttgcatccg gtggcggatc
    34441 gcaagaactg accgtaagcg atatagatgg cgacggacgc cctgatatta tcgccgccaa
    34501 taccaacggt accatctcgg ttttccgcaa tcgaaacaca cccggttcta tcggaacaca
    34561 aggatttgcc ccgtatattg atttagccgc cggagccaat tcagagtccg tatccgctgc
    34621 ggatatagac ggtgataata agccggatat tatctttacc aatcgtaacg acaatacttt
    34681 gtccatatac agaaatcaat ccacaccgga tactttgacg gcatcttcat tctccggttt
    34741 ggtaaacttc acggcggcaa cgggaccggg aaaaatagcg gtcgccgatt ttgacggtga
    34801 tctcaaaacg gatattgcaa ctgttaaccc gggatcggca accttttcag tattccgaaa
    34861 taccgcaacg ccgggtctta tcaatacttc gtcattggcc gaccgcgttg attttatgcc
    34921 gggtgcgtat cagatttcac tggctgccgg tgacatgaat agcgacttaa aacccgatat
    34981 catcagtgtc gcagcgacac taagtaaaaa caacaccagc gcgggatcca ttacctccgg
    35041 ctcatttagc accgcattca cattcaacgt aggaacacaa cccaacgatg tcgaattggc
    35101 cgatctcgat ggcgacggat tgctggacgt atccatgcta gattacgttg ataaaaaatt
    35161 gtacatctat cgtaatgcta tggccatcgt accgcgaatg gccgattcat tggcattggt
    35221 ggatttatac gatagcctga acggcaacgg ttggacgaat aaaacaaact ggaaatccgg
    35281 caaccccata gatacatggt ttggcatcac ggtgaccgat ggtcgcgtct cgcatgtcga
    35341 actcgggaat aataatctga gcggatttat accgtcatcc gtcggtaata tttcggccat
    35401 caaaaattta aacttattta acaacaacgt cggcggtccg atcccagcca cgatcagcgc
    35461 actcacagag atagtatctt taaacttgca aaacaatcaa ctgaccggca gtatccctgc
    35521 gtccatcacc gcactcacca aactgaaaca cctgtatctt gatttcaata ccctcagcgg
    35581 agcaataccg tcggatattg ataacctcac cgatctggag accttgtcat tatatcaaaa
    35641 tcagttgtcc ggcaccattc ccggcgggat atggaatctg cataaattga atcacctcgc
    35701 gttggggcct aatcccggtc tgagcggaac tctccctgcg caaatcgaaa atctcgacag
    35761 cctgcaaagc ctctttattt cctcctgcgg tcttacggga agcataccgc cggaaatcgg
    35821 aaacctgaac aaaatcctga tgatttacgc ctcggataat cagttcacag gcaccatacc
    35881 ggcatccatc ggaaacttga gtttgcttcg tacactgcat ctggcaaata ataatctttc
    35941 cggaagttta ccgtcttcat tggctaatct gcatcgtctg gagtcgctcg acatcaaaga
    36001 aaataaattc actgatcttg gagatttgaa tccggtggat tcgctcaacg tgctgatgat
    36061 agagcaaaat caatttacat ttgaagacat cgaacccaac ataaccgcgc cccgccagtc
    36121 ctttacgtat gcccctcagg acagcattgg cacatacaaa gatacggcgg ttacggaaaa
    36181 cgcggcgact tcgttggccg tcagtgtcgg cggctccggt aatcaatatc tttggttcaa
    36241 agacggttcc tctaccggca ttactactcc ggtgataagt tttggttctt ttgcatttgc
    36301 aaatgccggc acgtatcatt gccaaatcac caatacggtg gcgacggcac tgacattata
    36361 ttcgcggact tacaccgtaa aactccgcgg accgtcaatt cccacaggtc tcaccgcgtc
    36421 ggcccaatcc gcatcgcaga tcaatttatc atggagtgca agtcagaata atcctattcg
    36481 ctaccgcatt tatcgcagtc ccacatcggg ctccggtttt gtgcaggttg attccgtttc
    36541 ccatccgacc gtggcgtaca ataatacagg actgactgcg gatacggatt attattatcg
    36601 catcacggct gttgacgccg ccgggttata ctccgcggcg accacacaag catcggcgcg
    36661 tacgttgcaa acagcggttt ccattacttc tgtttcattg ttagttgggc ccacacagcc
    36721 caatgaaaac accatagtca ccatttccgt tccggtatcg ggttctgctc cggtcgtaaa
    36781 attatactac gccaaaaatt cgtccgtcaa tccggactcg accgaaatga cactgagcag
    36841 cggtccatac gtcgcaagca ttccggcttc gtttgtgaca gcccagggat tgtggtttcg
    36901 catccgcgcc tataataaca cgggttcgga attttatccg tcttcgaccg ccaaacatcc
    36961 gatcaacgtc aaggccggtg cgtcaacgct ctcggccatc cgatcttccg gcgcttaccc
    37021 gtccggcatg atcgccgacg gttactcgac cttcggttta ccgatggata ccagcgtcgg
    37081 catccctgca ttgtttggtt cacagatttt taacagcaaa aatgaaccca ccaactggcg
    37141 aatccaaaaa tatgccgacg gcagttttac ggatgaaacg tcagctttta catcgggcac
    37201 gggatatttt gtatatcacc ggctcggctc ttccgtccaa gtagatattc ctccgggttc
    37261 gctgacacat gcggccggta catttgaaaa ctttgtgctt aagccggggt ggaatctcgt
    37321 tccgtggccg tacacctttt ccgctaattt tacgataacc gatgaaagcg cgatagatcc
    37381 cattcaaacg cagattggcg gcaattggca aatcgtatcg tcattcaaac cttttggcgc
    37441 ttacgctatt aaaaatacta cggcttcaga tattttagcc ggcgacgtaa tccagtggac
    37501 gctttcaact tctaaagtga cggttgatta tgatttgata tccgatagtt ttgtccggtt
    37561 aagcgcggta tcgggccgat atttggataa tgataatttt gccggttttt ctgatgcggc
    37621 gcgggacggc gatgatcctt tggacaacga agagccgccg gcaatcggac cgggcgtgaa
    37681 tctttatttt acaaacggta ccgaacaacg ccaatacgcg gatattcgtt cacgcggtga
    37741 cggtcatgtg tgggattttc atgtaaatga tcgcaataac ggaaagagaa ttaatttagc
    37801 atgggatttg gctcatatac cggaccgcat gagcgccgta ttgataaata tcgcggaaaa
    37861 taagtggatc aatttgggtg aacaaacatc ttatgcattc agtcccgtta aagaaaatcg
    37921 ctttaagtta attatcggca caagtgattt cgtccaacaa acatccatcg ctcttgctaa
    37981 agaattaccc tcttcctttg ccttacacca aaatcatcct aatcctttta acccgtccac
    38041 gacgattcgt tacgatatcg cccacgccgg gcatgtacgt atcaaaattt ataacctgct
    38101 cggtcaggaa atcgtaacgc ttgttgatca atttgtaaat accggccgtt attccaaatt
    38161 atgggacggg cgtgatcggt tgggaaataa agttgccagc ggtatatata tttaccgcct
    38221 cgaaacttcc aagtttgttc gcagtaaaaa aatgctgttg atgaaataaa gcctacgatg
    38281 caaaaacaaa aaccccgacc gattggccgg ggatttttgt tacctgtgaa aggagttatg
    38341 gaaggataat taagatcaga agggtacttc gtcggtacgg acgaccgtat gttccgagta
    38401 ctcattttca gaaacgattt cagcgacatc gtcatgggtt tcgatcccgg tttcatccga
    38461 tgcatcgtca tcatggacgt tttgttttga cgatcggctg cccaacatct gcatcgtttc
    38521 ggcgaccacc tccgtcaacg catgtttacc cccgctcttg tcatcccacc gacgtgtttt
    38581 catcggaccc tccacgcata ccagagaccc ttttttcaaa tactggccgc acacctccgc
    38641 cagacggctc cacgccacca cgcgatgcca atccgtattc tgcaccagtt tcccctcttt
    38701 gtttttaaat acctgcgtcg tcgctacgct aaacgacgtg atcggcgtcc ccgtaggcgt
    38761 atagcgtacc tgcggatcac ggcccagacg accgatcaat acggcgcgat tcagacttcc
    38821 tttgttcatg acttgttctc cttgttttgt ttttatgtgt tattagtaaa ttctttttcg
    38881 tttcgttttc atcatcgtaa cgttattaat ttatagtctg ttggcggccc ttttctgtca
    38941 atttttgaca atatttgatt tcaatattat ttttaatgac atttcaacga ttaattttta
    39001 ttgggatgcg tagcatgatt aatagtattg atgatcttca gcgtcagatc gaaatcgccg
    39061 cgctgattta tgaattcccc gacaaatatt ccgaaataga cttagccgaa aaatttcata
    39121 ccagcgaatc ctccattcac cgcgatatgc gtgcacttcg ggcgatgggc atcaccgtca
    39181 attcccgcaa acgcgcgttt cgcatcgaac tatccctttt gcagatcaat acgctcatca
    39241 cgacatattt cgctttcggg aataatgaaa cgatcaaaaa tcttccgctc gttttggagc
    39301 gatttcaaaa caaaacgttg agtttcttcg ttgaaacgat ccgcgcgata cgccaaaagc
    39361 atgtcatcga gatcgaatac cgttctgcta aaaccaaccg ttatcagtgg cgtaccgtca
    39421 ctccggttac gttttacaat gccggaaaaa cgcattacct gatcgcgata caccaagaca
    39481 cggctaaaat gtttaccatc gagcgcattc agaactttcg tttttcgcgg caggcatcct
    39541 ccatcaaaaa tgtcccaacg ctcaacgaac tgtttcgcca ttcctgggga tcttttaccg
    39601 gcggtaaact gaccgacgtg catctgcgtt tcaaagatga tctgggccaa tacatggccg
    39661 aaaaattttg gattgaagag caggcgatga aacaaagcga taacgggttt gaaattcgac
    39721 tcaaagtcaa actgtccaat gaatttatag cgtggatttt gggatggggt gatgcagtga
    39781 ccgttttggc tccggacgaa ttgcgccaaa gcgtcctcga aaaggcaaaa agcatcgtaa
    39841 atcattataa aaaatgatct tataatgatt aaaaatagct cgcattttta taaaaaatgc
    39901 gttgctaaat agaacccgct tccctatctt tgcgggctat tcgtgaaaca tcatacagtg
    39961 ttccacatcg tttcaggaga atgaatgggc agaattattg ccatagccaa ccaaaaaggt
    40021 ggcgtcggta aaacgacgac ttcgatcaat ctttctgcga gcctcgccca tgccggcaaa
    40081 cgcacgctta ttattgatat ggatccgcag gccaattcca cgaccggcct cggttttgat
    40141 ctcgaaaaaa tcgaacgcgg ttcgtatgaa gttctgattc aaaccgttcc ggcaaccgat
    40201 acgattttga aaacgatgat gcccagtatg gatctccttc cggcgcacat ccgccttgtc
    40261 ggcgccgaga tcgaattggt caatctcgaa gaacgcgaac acatgttcaa aaaagccatt
    40321 gctccgattc gtgatcagta cgaatacatt ctcattgatt gcccgccgtc gctcggtctt
    40381 ctcacgctta atgcgctatc ggccgcggat acggtactga ttcccattca gtgcgaatat
    40441 tatgcgctcg aaggattacg ccagttgctc aataccattc gcctcgtcca gcgtcacctc
    40501 aataccgatc tgaccatcga aggcgtgctg atgacgatgt acgacagccg cctcaatttg
    40561 tccaatcagg tcgtgggcga agtcaaagaa tatttcaaag acaaagtttt caaaaatgtc
    40621 attcaccgca atatcaaact cggtgaagcg cccagctttg gtaaaccgat catcctctat
    40681 gatcctgcgt ccgtcggttc tcaagactat atgggcgtgg ccaaagaagt catcgccaac
    40741 gtcatcacgc cgcaaccggc ataacacaaa ttttatatga attttacgcc caaaaaaaat
    40801 acgggtcttg gaaaaggcct tagtgcgctt attcgcgaag aacttaatgc cgacggtacg
    40861 ctcaaagatg aagtgcggag cgatacgccc atcgctaaaa taccgattga ccagattcgt
    40921 gcaaacccct ttcagccgcg taaggaattc gaaccgcagg cgctggaaga actaaaacaa
    40981 tcgattctgc aaaacggtat cattcagccg atcaccgttc gtaaaacggc gaccggttat
    41041 gaattgatcg ccggagagcg ccgttttcgc gccagcaaat tggccgggct gactgagtta
    41101 cccgcttaca tccgtgaaaa tgtcaccgat caggaaatgc tcgaactggc catcatcgaa
    41161 aatgtccagc gcgaaaaact caatccgatc gaattggccg aagggtacca gctgctgatc
    41221 gaaacctgca atctgacgca agatcaggtc gctcaaaaaa tcggcaaaga tcgtacgacc
    41281 attaccaata ttattcgttt gctaaaactg ccggccgata tccaggaaag cctcaaaaaa
    41341 gaagaaatca ccatcggcca cggacgcgct ctgatcgcac tcccctccga acagcttcag
    41401 ctcaaagcat ggaagcagat cgtggacggc ggcatgtccg ttcgcaaaac cgaacagttg
    41461 gtcaaaacga cgcttgagga gctcgaagga aaaagccaca aagtttcgcc gaataaatat
    41521 gaaatcgaat ccgcgcaacc ggccaacccg catatcgcgg atgtggaagg acgcctgcgt
    41581 atgaagttag gcaccaaagt gcgcctgcat catcacaaag acggcagcgg ctccgtagag
    41641 atcgaatatt attcgaatga cgacttgcaa cgcgtcatcg aaatgatcga aaccatccgg
    41701 ggataatcat gggtgtgggt gaacatatcc gtaagcaaat cggccaatcc aaaaaacgcc
    41761 ctacggaact cgtcgcagag ctgaatgaat ttctaaaaaa aatcggcaaa aaacctaaac
    41821 aggaaaacaa tgaacgaata cgaaaaattg ttggtcctgc tgacaaaaaa taaaatccat
    41881 attatccggc tatcatgacg ggtacgcccg cgaactttcc gtagaagatt ttcctgatga
    41941 agaaggcgcc gttcgcctct ttgagatttt tccaatagat atttttacac ggataaacgg
    42001 caaacgttat tccgatctca cctctcatat tcgttttcac gctttggacg ggcacaaaat
    42061 tccttttttg gatgcttcca tgttggtttg gttcaaagaa cagtcactcc gtgaaagaga
    42121 tcaaatggat gtttcggctt tgaagcatct tttgaaaaac aaaacaggta cagacgaaaa
    42181 ctgattttgt ttttgttcga ttataggaca gcaggctgtt tttgcgcctg accaaaaata
    42241 tcctcgtaac ggatgctcaa ccaatacgcg cgtgtttttc caattaccat acgttgacag
    42301 aattttattt ttatgccctg tacgcctgtc actgttcgaa ccatcctcaa caaaaccaaa
    42361 cgccgcgatc cgtggttttt ggatgactat accctcaatc catacagcgg ctgttcgatg
    42421 ggatgcgtat tttgttatgt acatggaagc cgatacggta cacacaccga aaaaaaatta
    42481 tcctataaaa ccaatgcgcc tgaactgctg gataaacagc taagccttcg cgccaaacgc
    42541 ggacaatatg gattcatcgt gctcgcttcc tccaccgacc cgtatgtaca tgcggaaaag
    42601 gaactcgggc tcacgcgccg attattagag atcatcttgc accaccgatt tccggttcac
    42661 atcatcacgc gttccgatct gatattacgc gataccgata ttttgatgca catcggtaag
    42721 actgctattt tgcccgaaga cttgttaggc aaagtaccgt cgggcgcact catcacattt
    42781 tctttttcgt cggtagatga tcgcacggcg cacatcttcg aacccggtgc gacaccgccg
    42841 tcgcgccgcc tgcaaggact gaaacaaatt ttggatgaag gattttacgc gggcgtcagt
    42901 ctgatgccgc tgctaccgtg gatatccgat acggcagatc aactcgaact atcgttttcg
    42961 acgtttgcac aaatcggggc acggtatatt tttccggctt ctttgacttt gtacggtgat
    43021 cagccctccg acagcaaaat ccgcacactc cggacgattc aacagcatta tccgcactta
    43081 atggataaat accgtgggta tttcgaccgt tctgctacga tgcccgatta ttatctgact
    43141 gcgttggaag aaaaattgaa tggactatgt gaaaagtatc ggctaccgaa taaagtacat
    43201 ttgaatgatt agataactaa atctatttga gagtattttc taaagttatt ctaaattcgt
    43261 tttcattatc atagttataa aatggaacgg ccgttattaa atatgtatcc gtatggattt
    43321 tgatagcttt ttgttcattt ctaatttcct ttaaaaaatc ttccttccat ttgtcatagc
    43381 ttaacaaatg atttcctttt ggttctatga aaacctgaaa agtcatttgt tctccatcac
    43441 gttgtttgca gaacagcaaa aagtcgggtt caaatgctcg tccgagtttg tcaaagattt
    43501 tgatctctct ttcgtttcgg attaagtaaa tattctcaaa cttcaaattc agtccttcaa
    43561 atcgtctgga aaaaagttct acaaatttct tctcttcact tgttccataa ttggcattgt
    43621 aaacatacca aggctcgttg gctactaaag tttcttgccc gtctgctcgt acactgtctt
    43681 tgtaaacctt gatttctttg tctttgaaaa ctttgtgaat aggttctttg atatagtccg
    43741 aagcttcgta ttcagttgaa ttgcttttta tgtctgcttc aatgctttgc aaaagcccgt
    43801 ttaaagcttg cagataatcg aaatggttaa tttctttcag gcggttagct gttccgttaa
    43861 atgtgatttc taaacttgct aacaattctt tgctgtcaat aaaattggaa agtgaaccaa
    43921 cactcggaaa gtaatacgac aagctgtcga agtagaaaaa cggattttga cttaatgcaa
    43981 aacgaattgt gtttttcgga atatcagtca gctttacatc tttactttta atgatttctt
    44041 cacccgactg tgtcggctcc atttcaaaaa aagcgctgga cattctcccg acacccgaag
    44101 aaagtatgtg acgatagttg gttcttttga cgcctaaatc ggcaaatgat ttgatattat
    44161 cagaacgttt tagaattttc ttgttgaaga atacgtgacc atctcggtaa aactccgttt
    44221 ctttaaagtc aatcttaagt ctaagctgtt tggttacaag attttcttca tcttcataaa
    44281 tacccgaatc tacaagcgcc ttctttaatt ccgaaatgta tcgactatcc tcttttgtgt
    44341 gataatacaa ttcttccaac gtttttaaat cattggaaat gtcatcatca aattttcggg
    44401 tgaaaacatc atgaccctct tctatagcaa atgggaaata gcgcgctcct cgcccgatta
    44461 attgagcttc tgaaagcgtg gttattcccg gtttcccgtc ttttccgtca cgatcttcat
    44521 acaggcgaac aatgtcaaac aaattcaaaa cgtcccaccc ttcgttcagt ttctgaacgg
    44581 caaaaacggc acggattggg ttgttttcat cttccaatgt gttgagtaat atttggtttt
    44641 gctcagcttc tgcgtcattg ttggcactga ggcaattttc aggtttgaaa ttggcttgaa
    44701 tgcgttttac aatttcgttg gctgaaatgc cttttgcttc aaaaaatcgg aaagcctttt
    44761 gaacgattgc aacagtagaa gtcttttgaa
//
LOCUS       DAOQGF010000012        42236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_376_length_42236_cov_7.076195, whole genome shotgun sequence.
ACCESSION   DAOQGF010000012 DAOQGF010000000
VERSION     DAOQGF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 42236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_42236_cov_7.076195"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PK937_03360"
     CDS             complement(<1..1718)
                     /locus_tag="PK937_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH31639.1"
                     /translation="MKILILDAVASDADALEKRLRRRSITVKVRRAHIKAVFLRELRE
                     FQPDIILADYTLPDLNGIDALTLAGDLAPKAPFIFVTAHNGEAVAVRCMKSGAFDYVL
                     KDKIEDLDHVLERAETFIQNNAVPITAHFEMAPEQFRRIAESTRDLITVIDIEGRRIY
                     TSPSYGDYLDTHQVEQGSSFFDEIVAEDLPKVKQTFYEVIHTGNAMVVEHRIMAKDRK
                     LMFVEAQWNPIFDNSEKPVHIQIASRIITRRKRAEDALREHVRQYRAMVEHIADAISL
                     INPYGIVLFTSRSTHRVTGYNISEFVSQNFFDFIHPQDRAEALEYFQQVQAKEGMAPY
                     TEMRIRHKDGNYIWIEGLAKNMLHDPNVNAIVFNYRDITEWLRSEAALREKDELFRAL
                     IENNSDAITLMDAEGQIKFSVHSVLGYDDAEAVGQNIFDSIHPEDQDRIAEVFEQLIE
                     TPSTSLVAEYRAMHKDGSWRWNECTAKNLLKEPHVRAIVINARDVTDRKLTERALSEE
                     KERLLVTLQALGDGVITTNTEGRIILMNPSAERIIGSSLTTTRGQTIKSVFPLLSENG
                     ENKTAHP"
     gene            complement(1838..2992)
                     /locus_tag="PK937_03365"
     CDS             complement(1838..2992)
                     /locus_tag="PK937_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31640.1"
                     /translation="MESIGVNSNVYAGGKVFHIQTAGNLSSLLVKAEIFDSGRIVTVH
                     QKKLDPEQYKYLDTDRFRDVVAAFHQDIAWEIELLFFIRDRIKTIKHAVSSNKIGLLF
                     LHKGLVDEAIAEFENAITINPQMVESYRNLGLAFVEKKETQKAIEVLHKAIELEPRYA
                     DLHNSLGLAYALNGELDTALQTYETALAINPNYLEVHLNIGMVILRMHAERFQSAVFA
                     PSSIKPTRVINELQKLMNARTEFINYRFFSFRFEKAIRQIQDNDFLAAILTLKDIREA
                     TQTPNLDETMHGFYLKFLFGGAGKDEKVLGEYRARLEKSVEEHPHFADLHNSLGVVYL
                     IQCRNLFIKAMSAFKKAYEINPSYKSAYKNFRLVQNDGREFLNLLRAILK"
     gene            complement(3141..5282)
                     /locus_tag="PK937_03370"
     CDS             complement(3141..5282)
                     /locus_tag="PK937_03370"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNH31641.1"
                     /translation="MTEQVRNRLFMAFVALFPIPFIVWCFFLMRSMTENQTQIQSHTD
                     KIARIYKLQQAVYGLEEVISDQFLAATDTSFDDWNRIYQKAWQASLGVDWTDGSVFDM
                     RNALPRIDNLYQRIHKRKFKRTSIIPDSIRVENLTGEMIDEIHRAAESLGISAEKLRT
                     RSIRYQESLSQDWHTAIKFLIIASAITILISVIAVFYRYSLQRNKKLALLLYRLNENV
                     SGILGNTPDGVCTVDEHFKVLLINEPFKRLFHLRHQIELHPGSNMIDHLKPDAQAAWV
                     IVLTRAMRGEKFMIEQQFQLPDRLVLAEVSFNPVLVNNKVNGTAIHIHEVTKQKRSQE
                     TADRKNKELQDTIERQFRMIETIVQTAHDGAMIVDAAGQFPFQNDVARDIMTTAGTPT
                     SLSLWSGIHGVFMEGSEQPIDNQKLLERARSGEVFTILVRNERYMEGQKFEVSASSLT
                     EGDDLSRIIAFKAPPEIIPVMQEVPVPEIALKGALEEIPQWAFTINREMAIQSVNTAF
                     EKRFNKPRYTTIGYDIRQLIHPDDQPAFESRIQEALPEHPVHTAFRFLIDGQATHMET
                     RIIGGALTILCVGYSIEDMLLAEAAEDMRSAPQPLFTFDCDAYGVITQIEGDRTLMLP
                     SYRSEWQGQSMFKLFEQDLPMQEAFYRALDGQPIQRNAAYGQQEVTIVYQPVFDGVGI
                     IAGLKIQIFDAAALLAFQEKIKTRLSEKASI"
     gene            5430..5900
                     /gene="tadA"
                     /locus_tag="PK937_03375"
     CDS             5430..5900
                     /gene="tadA"
                     /locus_tag="PK937_03375"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNH31642.1"
                     /translation="MDTHETWMAMAFKEAEKAYAKNEIPVGAVIVLENRIIGRGHNQV
                     EMLQDPTAHAEMIAMTSAVATIGSKWLYGATMYVTLEPCSMCAGALVLSRMSQLVFGA
                     GDPKTGACGSLHNIVQDERLNHRIEVLRGVMDAKCSAILKDFFAKLRKPAPQDN"
     gene            6047..7909
                     /locus_tag="PK937_03380"
     CDS             6047..7909
                     /locus_tag="PK937_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31643.1"
                     /translation="MTIRSVLLWCCFVLCLPAVQAQKVKLKTGSVKEKKDEKKENKTE
                     TTSSGTFKVGDVAEGLETDGKWYKVDILKVEADKYFIHWQGYKSTYDRWIEAGKVRAI
                     GSSGSASANASTSAASTASGGKTKVYGTGDGGFKVGEYAEVLDRDGKWYEAEIQKVNE
                     GQPNEYFVKIENYNGFWVKESNVRAKSSGTSSEPVGFKVGDVIEAVDFGRWNKGTVQS
                     IRTTNSGNKMFTVAFDNGGSKEFTENKLRVIPVYNFSGSNYKTGQYIRIWYSGRDSLD
                     APIVDIKDNKLLINYGGYGAKWFDFDAKEIRNDARTAAIAASKATEEQFFQDCNKYSE
                     AIRIFGYLYNKKHDTRFVLYNQGLTSPRPSDVGPMLKQMNELDALIKSKYPNIQNTEQ
                     TWTTELSKNPGHWRVIAEERNELAQSVVTDMIKDKISEATRILTNYEFLKNHEIGEFE
                     AGKGIGVTSSLQDILDNNGADFMKKEEEKIADVIKAAQEANLGQNEITAFREFLKKLS
                     VDVKSKFPSSLVSSQFNPALHDAAAEASAKAYIQKTYSGAAILKIGVIGNQVIEKNNL
                     GIPLYRYKRVGVLFRDSQGRCRFVPAYYREDYAGGGAYGTPYIEHHFTYYQSCN"
     gene            complement(8006..8947)
                     /locus_tag="PK937_03385"
     CDS             complement(8006..8947)
                     /locus_tag="PK937_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31644.1"
                     /translation="MRIVVTGGAGFLGSHLCDRLLAEGHEVIAIDNLITGSVDNISHI
                     RDKRFTFIHYDVTNYIYVEGPVDFVYHFASPASPIDYLELPIQTLKVGSLGTHKALGL
                     AKSKGAGFLLASTSETYGDPLINPQHEEYWGNVNPVGPRGVYDEAKRFAEAMTMAYHT
                     FHNVDTRIVRIFNTYGPRNRPNDGRVVPTFINQAIRNQPITVFGEGKQTRSFCYCSDL
                     IDGIYRLSQSSEHYPVNIGNPHEMTVLQFAQLIIRLTNSKSEIVYRPLPKDDPTVRRP
                     DISKAKRLLNWEPTVDIETGLLKTIEYFSEKMKREGK"
     gene            complement(8958..10751)
                     /locus_tag="PK937_03390"
     CDS             complement(8958..10751)
                     /locus_tag="PK937_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNH31645.1"
                     /translation="MNYRIVMCAGLLLAGLASEGRASMNKPSIEYTLRMPAPHTHYFE
                     VTMTMQGIKKDYIDIIMPVWTPGSYKVREFSRNVENVRATSGSQSLRVEKISKNTWRV
                     FSGKTENVAVNYAVYAYELSVRTSYMDASHGYVNGASVFMYADGYQSLTHTVTIELYN
                     EWKHISTGLKPVSGSTHRFVADHYDVLVDSPIEMGNQKILNFELQGVPHEIAIYGIGN
                     YDTEAMVRDMRKIAETTAAMFGKLPFERYVFIIHCVPGYGGGLEHANSTTLGMDSWSF
                     ADAAGYNSFMSLTAHEYFHLWNVKRIRPKELGPFDYTSENYTKLLWIAEGFTAYYDDL
                     ITRRAGYYTVDQYLRRIEGNIQEVQSAPGRFVRSVEEASWDAWIKYYMPDENSVNSSI
                     SYYTKGALLGLMLDMEILRATRGEKSLDDLLRWLWTEYDQKLGRGFTPEEFQKAAERL
                     AGTSLDVFFQNYVRGTAELDYNAALQTVGLRVKEIKSDAPYYGFETRNNGEFTIRTVV
                     RDAPAWHAGLNVNDEVLAVDGIRVTARTLQARLNEKKIGTSVRILIARDTQLQEIVIT
                     PSEMPARYRLERVSEPTAQQEQIFKKWLNLN"
     gene            complement(10787..12445)
                     /gene="rpoN"
                     /locus_tag="PK937_03395"
     CDS             complement(10787..12445)
                     /gene="rpoN"
                     /locus_tag="PK937_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNH31646.1"
                     /translation="MKQILSQQMKLSPQQVMLSSLLQLPITALEQRLKQELEQNPLLE
                     ESQELEELQDEEMELEQTQEKPELKQEQEKPEDVAKEEEVAIQAEKDKEKKEEEEVDW
                     AKILNDDSSFEIRMPRGQGEDDDDQEWIQPDKVSLSDHLLEQLRWTSLTEEQRRIAEY
                     IIWNLNEDGYFVMDDPLTGETPIADEVQRFTEAVGEFRDDTLLADKPDEPAEESERKT
                     THRKKTTPKIDAVQFVADEFQTTAEAVEKILKTIHTFDPPGIGARHLKECILIQLYRR
                     KESDGFYQNGTDITIRIIHEAYEDFINRRYDKVAKMLNIPIDAIKHSISEVLSINPKP
                     GEGYITPEQNYITPDAAVKKIDGKFEIILNDYDVPHLRINNAYKRMMLERNKTGKETK
                     DFIKNKLEAAKWLINSLYRRRDTIYRTIESIVELQQEFFEKGVHHIKPMKLEDVAVRI
                     GMDISTISRATNGKYVQTDYGVFELKYFFSTAMTNSDGEDVSTKQIKTLLKEVIDQED
                     KRHPYSDEELAKVLTERGTPIARRTITKYREQMMIPSGRLRKQI"
     gene            complement(12467..13270)
                     /gene="lptB"
                     /locus_tag="PK937_03400"
     CDS             complement(12467..13270)
                     /gene="lptB"
                     /locus_tag="PK937_03400"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNH31647.1"
                     /translation="MSETVQHEQPSLPALADYIDHQSDQKKTLRAEGLVKRYGSRTVV
                     NGVNVQVTQGEIVGLLGPNGAGKTTTFYMITGMIKPNHGSVFLDEKKITTIAMYRRAQ
                     LGVGYLAQEASIFRKLSVEDNIMAILETLPLKRKARRERLDQLLEDMRITHIRKSKGY
                     ALSGGERRRTEIARALVTNPRFLLLDEPFAGIDPIAVEDIQSIVQGLKSRGIGILITD
                     HNVHETLAITDRAYLLFDGRVLKSGTAQFLADDPEARKLYLGDKFKLRD"
     gene            complement(13267..14457)
                     /locus_tag="PK937_03405"
     CDS             complement(13267..14457)
                     /locus_tag="PK937_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNH31648.1"
                     /translation="MRSFCYFIITLCFFAVVHSLWAQQNEDYKPLKLDEADEIENQRI
                     DGKDVMKARGDVRFSHDTLTATCDQAAFFREMQSAILIGNVVLNDRHRTIYCEKARYY
                     ARQKRAVCEGNVIFVDRATTLVADSLVYFTQSEQLTAQGQVVIYDSTEEMTMYGERGF
                     YDAKRRYANATGHPYMIQFDSTHYKGQNIARLSRGIYTQPPPRDSVTGKPVRFSPDEQ
                     ITARGLFVEVYQDSHLVYVRDSVEFLREKLRTTSGKARYHTKKEFLTLQKEPIADYAL
                     NRMVGDSMIVQFRKRAIETMYVIGNAIATSSVDTLGTMVNTLQAKQITMHVKDKHLDV
                     MEAQGNAYNVYYLEGREGANRISGPNMSLFFDTKGKLRNFRVDGGAEGTYFPENLKNR
                     IEKP"
     gene            complement(14454..15071)
                     /gene="lptC"
                     /locus_tag="PK937_03410"
     CDS             complement(14454..15071)
                     /gene="lptC"
                     /locus_tag="PK937_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNH31649.1"
                     /translation="MKYWFIITYTTLLSCTALTEKEDLPAGEKPDRPGQEGWNSVAVF
                     SSGGVKDAELHFAHMQKWDTKQLTTFRNGLRVEFFEKGTHSATLTSDSGEIRSNTNNL
                     FAYGNVVIVSDSGVSMQTEKIYWDHQKQRIFADGLVTMTTDEDTLRGYQFESNKDLTN
                     WKMKNASGRSARDIDLRTGTVKPKTTRRNDTELDKEMDAMIKERP"
     gene            complement(15068..15826)
                     /gene="recO"
                     /locus_tag="PK937_03415"
     CDS             complement(15068..15826)
                     /gene="recO"
                     /locus_tag="PK937_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNH31650.1"
                     /translation="MAIITTEAVILRSTPYRETSKIVTALTKDHGKLSLIARGARDTK
                     SKYGGALELFNHISVIYYERANRDMFYISDASVIRGFTHIQKDLSRTYIAMAILEMAN
                     RVVHGNEEHQGFFELILQTLAGLHEEQRPKNGLLFFLMAMADNLGFTMDFENCDFCDN
                     RVKNKDILRFNSDRGRLVCGECPREFHVHDTPALSKESIAVMAHLARCRGRGVYNLTI
                     SERSTQEVFQVLLQHLQRHIDELRTLQTLAYLRL"
     gene            16228..16968
                     /locus_tag="PK937_03420"
     CDS             16228..16968
                     /locus_tag="PK937_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNH31651.1"
                     /translation="MSSNTKLSTKPRDGKKGARIVFASETLDLGVIREGDIVDGVFSF
                     SNAGDDTLKIQNVKASCGCTAALLSSDIIPPNGKGEIKATFNSRGKVGNIHKTITVFS
                     NSADNPVTQLNLVVKVEGNGSAHTGGMKKSNAMHPEVEGDMHGSYFEGKCANCHVEPG
                     VGKFGKDLFDVSCAMCHGADGRGAVAQPLRGGEFLNRADASLLQERIAKGSPSNVMMR
                     GFAKSAGGPLDETQIKSLVEYIGSWKHK"
     gene            16976..17389
                     /locus_tag="PK937_03425"
     CDS             16976..17389
                     /locus_tag="PK937_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNH31652.1"
                     /translation="MKPIKVLVAKPGLDGHDRGAKIIASALRDAGMEVIYTGLRQTPE
                     MIVQAALQEDVDVIALSILSGSHMTLFPKIMTQIKEAELDDVLVIGGGIIPDEDAAAL
                     EKTGVQKIFGPGTSTSDVVDFIRAWYEKSSRRVLV"
     gene            17424..18200
                     /locus_tag="PK937_03430"
     CDS             17424..18200
                     /locus_tag="PK937_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNH31653.1"
                     /translation="MPFPRLTKLQVKEYSEVLDKRTRKQKALFHIEGVRLFEEFSSSG
                     WEAEWIAVDTPFLEAHPQAAEFLKKKFGVCTWLASSLDIKKLSDTEHAQGIVAAIRKP
                     AAIDENGRAPLGNVLILDQIADPGNLGTMLRSADWFGVDHVYLSPSCAEAFSPKAVRA
                     TMGSIFRLHIVENASLRTTIETLLKKKYTVLAADMHGEKIPPKMLAPWALIIGSEAHG
                     LSDEVRSAPIQLMAIEKYGRGESLNAAVSCGILLHALTRS"
     gene            18280..19605
                     /locus_tag="PK937_03435"
     CDS             18280..19605
                     /locus_tag="PK937_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HNH31654.1"
                     /translation="MALTLIKNGTVITMNSARDIVKADILIDGAIIRKIGKIRVPSGS
                     RVRVIDAQGCIVAPGFVQTHIHLCQTLFRNLADDLELLDWLKLKIWPFEAAHNPASIR
                     LSAEMGLTELIRGGTTTIMDMGTVHHTDHIFEAIDRSGIRALCGKAMMDDCPGAPKGL
                     IETRAWSVEESLHLMRYWHRRSDGRLRFAFAPRFVLSCSEELLCEVRDLSEEYGLLVH
                     THASENRGELAEVIKKCGTPNVEYFHDIRLTGERLCLAHCIHLNETERHILRDTGTKV
                     MHCPSSNLKLGSGIAHVPELLGLGVTVSLGADGAPCNNNLDMFQEMRLAALLQKPRLG
                     PRSITAEEVYTMATLGGAKTLGLSDEIGSLETGKRADLIIMQLDEIHSRPEENLYSQI
                     VYSAKSTDVRTVVVDGKVLMKDRRMVTLDDESVRHRIGPELKKLLNRIA"
     gene            19642..20007
                     /locus_tag="PK937_03440"
     CDS             19642..20007
                     /locus_tag="PK937_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31655.1"
                     /translation="MYNVLEVITADKGIMSEYFILSCIEFFILLIVSERVLAARNGWA
                     NPSIWRDNTHIRMCYLIRKWSIPSLFLILNTVAVSSFAHHAHELAELFFMNVLVLMSS
                     AVLGLLLGESAVQRYRVGR"
     gene            complement(20004..20789)
                     /locus_tag="PK937_03445"
     CDS             complement(20004..20789)
                     /locus_tag="PK937_03445"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNH31656.1"
                     /translation="MNHDWDNIKNWSLARGNSLTTGYTIITLDAIGSTNEYLKQLWRD
                     EKAQHGCLVIADTQTAGKGRLGRTWESPPGAGLWMSLLIKPSLPPDKHFAYSFAAAVA
                     VAEAIEQTIRLRPELKWPNDVLLCQKKCCGILLESVTTGSEPWIIVGIGINVTQESFP
                     DALSDTAISLQQAHAAPVHRLQLLQNLIPALQSNFSSDSREILIKWQSYCRMFGTMVS
                     VFQQDSVMEAKAVGLAPDGGLVVENEGKRIKVYAADVKIRLKN"
     gene            complement(20779..21564)
                     /locus_tag="PK937_03450"
     CDS             complement(20779..21564)
                     /locus_tag="PK937_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNH31657.1"
                     /translation="MKGFLHAALTALERYNRIAIATVVRASGSTPRAAGAKMLIFPDG
                     TAQFTIGGGLFESLVIKDALEVLQSGKPLTRTYAFTLDGQHAIGAVCGGSVEVLIEAM
                     HNTPQLWIIGGGHIGQALMRAAAPLHFTISLFEDRAEYARTPHDLPHVRAHHVAADYH
                     DMPEPGDDTFVCIVTKGYITDEAALRRVIRSRAHYIGFLGSRRKIHTVFENMKRDGFE
                     GKLFERIYAPIGESIHADAPEEIAISILAQIIRVKNQVFTVES"
     gene            complement(21561..22640)
                     /locus_tag="PK937_03455"
     CDS             complement(21561..22640)
                     /locus_tag="PK937_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31658.1"
                     /translation="MRSFFSYVIAMCIIIAACALIPVERFFHYIMDTGSIEQTVRQSG
                     LYGKLIQQATRNVTQHRQFKKLESTALALSTEEVQRLMTKTFAEDWFYTSLRTAHLDL
                     INPLNAEPSQNDHVGYIIITDKKDLLLENLAQIIENKMNALPECSPRQTLTLAGQYMR
                     NNGKITLDKIKLDCKPPQSLASEVRTIIRQEVSQGLTALPDTIYYLQWMDEKNSKMQH
                     KDFIEAGTTMRNVRLGSIILLFAGMLILVVINFNNKKQLLVRTALPLILTGLLLGGGV
                     WFGIRYFEDPAHAEIFKIEGPAAINKQPMSTDTANIILGVVKTFFLDYMRSIFYTCLV
                     IGGIGLMMLVSSRYVHDEHMESGAS"
     gene            complement(22643..23596)
                     /locus_tag="PK937_03460"
     CDS             complement(22643..23596)
                     /locus_tag="PK937_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNH31659.1"
                     /translation="MPVSSSKTLRRLDYFFILRPILFIPGWTTTLAGYMAATGQAKHW
                     PVWNGLWYPDMMGLFISTAMLMGSGFLVNQLRDIETDRINKKLFFLYDGSVNPRILIL
                     QSILLSAGCLVMASFWGERMLIVHFFGWLLIAILYNLPPFRLKDRAVTGLLANAGMGV
                     FALAYGWALSDQSWLLFLITGFPYFIFNGALCVLTMIPDTEGDAATSKKTFTVRYGVT
                     FSMYTAFVLFCIAAITAFILDQYVLLVVLVSVIPMFVFMVIRKTKADAIRALKFSIFF
                     FTSLVAMYFPFYIILLIFLFVCTRWYFKTRFDFDYPNLQGR"
     gene            complement(23777..23968)
                     /locus_tag="PK937_03465"
     CDS             complement(23777..23968)
                     /locus_tag="PK937_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31660.1"
                     /translation="MASKGRVALRALGFMILALIIASIAVIFNAPSILMGITGILFPI
                     GLFIMLLVAFYLLILSTKG"
     gene            24348..25190
                     /locus_tag="PK937_03470"
     CDS             24348..25190
                     /locus_tag="PK937_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31661.1"
                     /translation="MGDAKLHQLDFHLHHLPRLNRTEAQATLVPTDAVESVQAIFLSV
                     YRTYKSQSRRWISVTRIEVSFYNYTQFKHTMQIRDHTARVRISASARTEPAQFFEALA
                     HVLWSKIFGVRCPDAIMQYYKDAEWRLSREKETETKPTIKRFAPPKGRVYDLAQIKDQ
                     LDAVYFDRSMATVQIGWSLQKARTRLGHYDRSAQMIIMSPVLDHPDVPPAVVTAIVYH
                     EMLHHKHPVVYDEGRQIIHSRAFQQEEKRFVHYAEAQHWLKTEYPQFLTRRRGPRWFK
                     SWFT"
     gene            25197..26090
                     /locus_tag="PK937_03475"
     CDS             25197..26090
                     /locus_tag="PK937_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31662.1"
                     /translation="MAKKLLITGGTGTLGSHLVRQAIASGAWDEVHATNYSQRPNFHK
                     VFWYPMDARQPVGAIIERIAPTHILHTLALSSPDICEKNKLEAWQVNVSVTQSLAEYA
                     AQNKARLIYTSTDLVFDGTQGNYAENDTPQPLNFYADSKLEGEQAVREVMKDGHFVIV
                     RLANLYGPNLNGKENFFTQMTKALRQSSELKLFTDQIRSVMSASNAAACLLELADLSF
                     EGLLHLGGPQAVSRYEFGQILAHAMQVKNPSIKKITMAQMPGGAKRPANVSLKITQAQ
                     NILTTKLLAPEEGLKREIEGY"
     gene            complement(26102..26356)
                     /locus_tag="PK937_03480"
     CDS             complement(26102..26356)
                     /locus_tag="PK937_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31663.1"
                     /translation="MTDEFKTQCVTLAREIQEALTTLEVATDPSSEERERIEAKNVVA
                     NVLRSYKSLIENLSGTDKTEASKLLHKKIEDMALKAKHLR"
     gene            26486..29341
                     /locus_tag="PK937_03485"
     CDS             26486..29341
                     /locus_tag="PK937_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNH31664.1"
                     /translation="MKSLLSAIRLQHFYGIALHRTGGDIWEDAITHVAAAQFIDGKIT
                     VVLDEACASDDTREAVLRRLQDTIGDVPLVLFSSADIEFLHASFHAMYDNKDRASPFL
                     MNECWDLQRLASTLLPVISFATLEELAVYFTFPEGQNPAPSEAVILIFYRLIESLARM
                     EPRIVSQANRFLEHSKSPLKRILRGLADYIRDGKNIHFIFPEVQWPTPAPNVIGVADG
                     GFFSDTIHSVPDSDIRRFFSPQGGLSRSMETYEERAEQTRLTVAINSAFNASQHILAE
                     AGTGTGKSLAYLIPAIHWASYNNALGEQVVISTHTKNLQDQLFYKDIPLLARTLPFGF
                     RAVLLKGRSNYICMKKWTHITADAGSHVQPQEREKLLPILFWIHKTRTGDISECVGFQ
                     AEANPGVWHKLASESAYCQAQRCSRSEDCFVRKIREEAKQAHVVVVNHALLFSDLVSD
                     NAILGDYHNLVLDEAHNVEKVAQNYLGAEFSLWAVRGLTSKLYERDQTETGVLIQLRQ
                     KLQKSRLSDVLQSAFLSQIQTGTDACVQFWLAAQTLFQRLTTEALRKTEFAMQSDKAV
                     RGERRVRYGPTDSLLLRVADDVDLMHEKATKLRQALDVLSETMRQWPTDAMDGAEEMR
                     DLIILRTDELDALLKTVDFLTEGANKDYVYWYEMPPKERSYDMRFYAVPLNIADILRN
                     TLLEKLKTCVFSSATLSVAGQFNYYKRRIGLDAMRSEKVQEFTADSPFDYKNQSRILV
                     PTFLPEPQSPKYNDACAELLKRITLETRRGMLVLFTSYQMMNSCYYQLRDPLNAAGIP
                     LLMQGRDASRTQLTEHFRRERVSVLFGTDSFWEGVDVPGSALEILVITKLPFEVPSDP
                     LVAAKMERIDAQGGKSFFDYSVPEAVIKFRQGFGRLIRSRSDRGVALVFDNRVVTKSY
                     GSYFMRSLPVQALRMPTVDGLIDNIDNFLGFEFLNQK"
     gene            29377..30456
                     /locus_tag="PK937_03490"
     CDS             29377..30456
                     /locus_tag="PK937_03490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH31665.1"
                     /translation="MIYKYPLNFQQVVNDLEKDSHNEPLFISGEALLVLKSFPDSVID
                     MAMTSPPYWGKRDYQNDGIGLEADPIQFIDNLLNVFNELKRVLKPTGSFWLNIGDTYR
                     NKSLLGLPWRIAIRMIDEQGWIMRNSVIWNKLKGSMDNSPDRLANVHENLFHFVKSKH
                     DYFYDTNAIRKKPKNVIIKNGSIISATGVSGVRYRRQIELSSELSEAEKKNAIEDLDV
                     MMNQIRLGELSDFRMVIRGHQRATHSDAESISGRARELKQKGYYFLKYHPNGSKPADV
                     WDIIPEDSQKRKIHYAPYPEDLCRIPIAATCPPNGIVLDPFCGTGTTMAVAKKMNRKS
                     IGVDLSSDYIQYANKRCEVWQMEMH"
     gene            30435..31286
                     /locus_tag="PK937_03495"
     CDS             30435..31286
                     /locus_tag="PK937_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HNH31666.1"
                     /translation="MANGNALSELYSYNTADDEVKWKNVIEKQYCPFISRKCLKNRKS
                     DAKQSIGSCSIQYGKESDTIIVCPFRFLERNKIFFDSIHLLRLHEPGNELHVINEFSI
                     PGGNVDYILASARDGKVKDFVGIEMQALDTTGTVWPSRQKLLHKFGVKVLKSDRESTD
                     TFGVNWKMTAKTILVQLHHKILTFESLHKHLLLVVQDKMMDYMTSQFDFSSLHDTLNG
                     DSMHFHMYEMSKKGHKFQLVLKNRKSTDADGIAKCLGLQANPQVELNIILETLTKSIS
                     EKTILKF"
     gene            complement(31297..32532)
                     /locus_tag="PK937_03500"
     CDS             complement(31297..32532)
                     /locus_tag="PK937_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH31667.1"
                     /translation="MKKQLLILGTGFGGFSLMKGIRSDLYDVTVVSTRNHFLFTPLLP
                     STTVGTIEFRSIIEPVRLARKDVTFYQAGATALHPEKRTVTCRNIADAQTFELHYDTL
                     VIAVGAVNNTFKIPGVAEYAYFLKELRDARRIRQRIIDNFERASTPGLDAAEMQRRLH
                     IVVVGGGPTGVEFAAEMADFLEEELEKGFPHLVGMAKITLIEAGKQILSAFDHTVSEY
                     AIKLFNRRRVTILTGKSVKEVTAQELTLHDGTQFSHGLVVWSTGNGPTDFVQSLSCPK
                     DRAGRILVDSFFRVKDNPDIYALGDCATTEEKSLIATAQVANQGGKYLANYLNQLAKG
                     KSAESLKPFVYKHLGMLAYIGDNRAVADFDATTSQGFATWIFWRSAYLTKLVSLKNKA
                     MVLFDWAKAFLFGRDVSRF"
     gene            complement(32539..33042)
                     /locus_tag="PK937_03505"
     CDS             complement(32539..33042)
                     /locus_tag="PK937_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31668.1"
                     /translation="MKKTIYTATILIFCCVAPLATAQTSWETHRFEPYRIEFKTPPHW
                     QISINTDGEKSFIECYSPDSEIYFFITVAENEKKSDPDIVLQYLKVGSLNSEFIRDEA
                     RKINNIDFKFSIGIKTVNNYSTYIRIGVGQYKDKIYMLESGFSRVDSDASEALLDQVI
                     SSFKAIR"
     gene            complement(33039..33506)
                     /gene="bcp"
                     /locus_tag="PK937_03510"
     CDS             complement(33039..33506)
                     /gene="bcp"
                     /locus_tag="PK937_03510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNH31669.1"
                     /translation="MALLKLDAKAPDFSLEDTHGHVVKLSAYQGQKVVLYFYPQDNTP
                     TCTIEACSFRDAHAAFKKNKIAVFGISPDSVKKHQNFTKKHELPFPLLADTEHRVIKM
                     YGVWGPKKLFGRAYQGVLRTTYIINEKGNIAFVFDKVHAETHAEDVLKAIKAL"
     gene            complement(33510..33725)
                     /locus_tag="PK937_03515"
     CDS             complement(33510..33725)
                     /locus_tag="PK937_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31670.1"
                     /translation="MTIRVTNAENGINEEFKSNNLDFIRRSYTEFDDVQKMSEEDYKK
                     LAILIVKKRYTDTRFDNLEWTVTTEND"
     gene            complement(33756..34598)
                     /locus_tag="PK937_03520"
     CDS             complement(33756..34598)
                     /locus_tag="PK937_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31671.1"
                     /translation="MKKLFFADRIKASTLWAQRRYYMTVVMFFFILSDLTAQMPYTWM
                     RSADSLAAQYNAAQAVDAYRHAVQKDSANCEARWKLAMQTIALGNDLPKCEEQEKLYA
                     EAFEWARVAVSCDSSSLAAWLTLATAAERAAMDEFGSRHSVLLAEMRFAAERALALDS
                     LSDEAEHLLGLWNRFVANASWFSRTTARLMGTAYGTAAISRSIEHFQRAIRLHRDEPR
                     HYLELGRTLVLIERWNEADSAFTKAAELPWQDRYAHRHRRDARHYRELLREGRYAELK
                     NSIE"
     gene            34760..38380
                     /locus_tag="PK937_03525"
     CDS             34760..38380
                     /locus_tag="PK937_03525"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNH31672.1"
                     /translation="MEETTFTEKEISELEKFGMNSWFVEELLASYKNNSTMVNESWQD
                     FFDRMSGKPVTAKPAEISNGNGSLIGNIPMPKPAAGEEAVVLRGVGAKIVENMSYSLS
                     LPLATSFRTIPVKILEENRAIINNFLKKSNRGKLSFTHFIGWAIVKALKAHPVMNNAY
                     TVMNGEPHVIRKPQINLGLAVDQEKKDGSRSLIVPNIKNAGAMNFVQFFAAYDDIIRR
                     ARANKIEPADFMGTTISLTNPGTVGTVSSNPRLMVGQGAIIATGAIDFPAQYHGMPES
                     NLSAMGISKVMNVTSTYDHRIIQGAESGLFLKKLHELLLGEDGFYDEIFEALQLPVKP
                     AQWQVDNYPMAFGKSQDFELIEKQARILEMVNMFRVRGHLLANLDPLTPQMLYHPELD
                     SQTYGFTVWDYDREFITGGLAGKKKATLKEILDILHQTYCDRIGVEFRHIQSPEEKAW
                     LQERMESSRNTGKYSKEEKKQILKKLIEAESFERFLHTKFVGSKRFSLEGSETIIAVL
                     DAMLQWCGDDGVKEVVLGMAHRGRLNVLANIIGKSYQKIFSEFEGHHDPHSAQGSGDV
                     KYHLGATGNYPTRSGKSVKISVAPNPSHLEFVNPVVEGIVRAKQTRMPGEYNKNVMAV
                     LIHGDAAFAGQGVVAETLNLSQLKGYRTRGTLHLIINNQIGFTTTPEAARSSTYSSDV
                     ARMVQAPIFHVNGDDPEASLWVTQLAFEYRQKFEKDVVIDVLGYRRHGHNEGDEPGYT
                     QPVMYKKIKDHDSVKEFYGARLIGEKVITVDEFKAMESDFYQALNTALDAAKQKAIPY
                     QSETPLAISEEAAQAAPTSQGTAVSGELLAKVVEGLTRLPHEFTLHPKLQSFIDTRKK
                     FMHDDGVTVDWAFGEALAFGTLLMEGTPIRLSGEDISRGTFSHRHLALTDMNTDLDYI
                     PMNFIEINQSKIEAVDSLLSETAVLGFEFGYSIADPLTLVLWEAQFGDFSNVAQPIID
                     NFIASCKSKWSLSTSLVMLLPHGYEGQGPEHSSARLERYLQLCAEDNMIVCNLTTPAQ
                     YFHALRRQIRQKTEKPLIIMTPKSLLRLPDARSYKHEFTNGTFHEILDDATVTDRQAI
                     TRVIISAGKVHYDLVKFRIKNNRTDVALVRLEQIYPYDADTMKRILASYPNAATVAWT
                     QEEPRNMGSWNFLLERLTNDLTAKQKLVYAGRKASASPAVGSLSVHQAEQEQLVRDAF
                     EL"
     gene            38422..39828
                     /locus_tag="PK937_03530"
     CDS             38422..39828
                     /locus_tag="PK937_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH31673.1"
                     /translation="MQDYRFSELFEAQKKHFQSHIKSSRPDARIVKLKKIRTWITAHA
                     QDIRDAIYEDFRKPSVEVDLTDIRPVLMEIEHALVHIRSWMASRYVSTPLLFFGTRSK
                     VVYEPKGVCLILAPWNFPFMLSVGPLISAIAAGNCCILKPSEMTPQTSRLIARMIAEL
                     FNPNEVCVVEGDHVAAEALLKLPFHHIFFTGSPAIGKKVMHAAAEHLSSVTLELGGKN
                     PVIVDETADIEDTVSKLLWGKFMNGGQSCMAPNYVYVHHTIYTAFCEALVAMYARMFG
                     TANEPSPDVAGLVSDRHYERITSLIRTTVAQGAKTLAGNHFDAARRYVAPTVLGNVTT
                     DHAVMQDEIFGPILPLLRYTHRDEVIAYINTQERPLNLYIFSTDMAKADAIIAQTTAG
                     STCINETTLNFAHPDLPFGGVNTSGIGKAHGYYGFLAFSNERGILKQRRGLTTMKLVY
                     PPYTERVKKIVKLVMKYL"
     gene            39913..40821
                     /locus_tag="PK937_03535"
     CDS             39913..40821
                     /locus_tag="PK937_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNH31674.1"
                     /translation="MKQTKNFPLRVLTFNVWGLKVGPLHIARDIKARMDRIASALHRS
                     ESDVIVLQEVWCASIAKFLIRESGYPYHVYQPGRGTLRGPLGNGLLMLSRFPIVHTQT
                     LRFHHCTSPDEWFVGKGAVAADIHTAQGVVRVINTHLGSGKRPLHVTMRLQQLRQLQS
                     WVAGLEHHMPSVIAGDFNFSPSSLEYCVFRHWATHHFRESLADTFAVRHGQADGHTYF
                     VDRSYHVKPKIHDRNERIDYVFLLTPKQSRLDLTVEESTRILNDAEHPYSDHCAVLST
                     VGIRIKPTLREIMVSDPMIPARKAIL"
     gene            40818..41456
                     /locus_tag="PK937_03540"
     CDS             40818..41456
                     /locus_tag="PK937_03540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH31675.1"
                     /translation="MSELQTVITVLSGDFETYGYAGLFGLLILTTILPLPLPEELMCI
                     TTGYLIERGVMAPVPAILTMFMGIVASDVYQYYFAQILGNKLMRVRPFSWILNEERIR
                     KAEALFLRQGSKVLFFGRWVYGLRSNIHFAMGLLRFPIRRFIVIDAAVVSIQSLSLVL
                     IGYYSPLYMNHWASHLMTLQWWLYPLSMIALSVALSGMIFKAWKKRQREVIL"
     gene            complement(41681..>42236)
                     /locus_tag="PK937_03545"
     CDS             complement(41681..>42236)
                     /locus_tag="PK937_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31676.1"
                     /translation="LMPRYLHFAFSAIAVAGMLVLILGMREKKDNALSSFMKRWGGTW
                     FLIGTVANTVVGFMFYFAIPEALRKAFTEGAGQMLFLSSHALFIIGTLAIAAVTFLKA
                     NNMIGWVGIAAMLVGIAEKVISRDLLRQEYLKQVNWTHAVLQTKPQNDVIALFFISFV
                     AGIGLLLWVYMRYKKEVAAHTQQS"
BASE COUNT        10893 a         9608 c        10440 g        11295 t
ORIGIN      
        1 gggtgcgccg ttttgttctc tccgttttcg gataacagcg ggaaaacgga tttgatagtt
       61 tgtccgcgcg ttgtcgtaag tgaagaaccg atgatacgtt ctgcgctggg attcattaat
      121 atgatgcgtc cttcggtatt cgttgtgatc acgccgtcac cgagcgcttg cagcgttacc
      181 aaaagccgtt ctttttcctc ggagagtgcg cgttccgtaa gtttacgatc ggtcacatcg
      241 cgggcattga tgacgatcgc acgcacatgc ggttctttca gaagattttt tgccgtacat
      301 tcattccatc gccaggagcc gtctttgtgc atggcgcgat attcggcgac aagggatgtg
      361 ctcggtgttt caattaattg ttcaaacact tcggcgatac ggtcttgatc ttcggggtgt
      421 atggaatcaa aaatattttg cccgacggct tcggcgtcgt cgtatcctaa aacggaatgc
      481 acgctgaact tgatctgtcc ttcggcgtcc ataagcgtga tcgcatcgga attattttcg
      541 atcaaggcgc ggaataattc gtctttttcg cgcaacgccg cttccgaacg cagccattcg
      601 gtgatgtcac ggtaattaaa aacgatcgca ttgacattgg ggtcgtgtag catgttttta
      661 gcaagccctt cgatccaaat gtagttgccg tctttgtggc gaatgcgcat ttcggtataa
      721 ggcgccatac cttctttggc ctggacttgt tgaaaatatt ccaatgcctc ggcgcgatct
      781 tgcggatgaa taaaatcaaa aaaattctga ctgacaaatt cgctgatatt atagcccgtg
      841 acacggtgcg ttgaacggct ggtaaaaagc acgataccgt agggattgat cagcgaaatc
      901 gcgtcggcga tgtgttccac catggcgcgg tattggcgca catgctcgcg taaggcgtct
      961 tcggcgcgtt tgcggcgtgt gataatgcgc gaggcgatct gtatgtgcac cggtttttcg
     1021 gaattatcaa atatcggatt ccattgcgct tcgacaaaca tcagtttccg gtctttagcc
     1081 atgatacgat gttcgacgac catggcattg ccggtatgaa tcacttcata aaaagtttgt
     1141 ttgaccttag gtaaatcttc cgccacgatt tcatcaaaaa aagatgagcc ttgttccacc
     1201 tgatgtgtgt ccagataatc gccatacgat ggactcgtgt agatgcgtcg tccttcgatg
     1261 tctatcaccg tgatcagatc ccttgtactt tcggcgatgc gacgaaactg ttccggcgcc
     1321 atttcaaaat gtgctgtgat aggaacggcg ttattttgga tgaaagtttc ggcccgctca
     1381 agcacgtgat cgaggtcttc gattttatct ttgagcacat agtcaaaagc gccggacttc
     1441 atacagcgaa cggccacggc ttcaccattg tgcgccgtga caaaaataaa aggagcttta
     1501 ggagctaaat cgccggccag cgtaagggcg tcaataccgt tgagatcggg gagtgtataa
     1561 tcggctaaaa taatgtcggg ttgaaattct cgcaattcgc gaaggaacac cgctttgata
     1621 tgcgcacgac gcactttgac cgtgatcgaa cgacggcgca gacgtttttc caaggcatcg
     1681 gcgtccgacg ccacggcatc taaaatcaga attttcatta gacggtgttt aaacgattaa
     1741 ttggaaccat tgtatcgccg gtgaatatac gcgcaaaggg cgtacttagg caatataaaa
     1801 ctaccactga aataccgtta atgccacagc acgatgctta cttgagtatg gcccgcaaga
     1861 gattgaggaa ttcccgtccg tcgttttgta ccaatcggaa gtttttgtat gcgcttttat
     1921 aggacggatt gatttcgtaa gcttttttga aagcgctcat ggccttaata aaaaggttgc
     1981 ggcactggat caaataaacc acgccgagcg aattatgcaa atcggcaaag tgcggatgtt
     2041 cttcgacgct tttttcaagc cgcgcacggt attcccccag tactttttcg tctttgccgg
     2101 cgccgccaaa caaaaatttg agataaaaac cgtgcatggt ttcgtcgaga ttcggcgttt
     2161 gtgtggcttc gcgaatatct ttcaaagtta gtatcgccgc caaaaaatca ttgtcctgta
     2221 tctggcgaat ggctttttca aagcgaaaac tgaagaaacg ataattaata aattccgtgc
     2281 gggcgttcat cagtttttga agttcattga tcacgcgcgt cggtttgatg gaagacggtg
     2341 caaaaacggc gctttgaaac cgttcggcgt gcatgcgcag aatcaccatg ccgatattga
     2401 gatgcacttc aagataatta gggttgatcg ctaatgcggt ttcgtaggtc tggagcgccg
     2461 tatccaattc gccattgagc gcataagcga gaccgaggct attgtgcaag tccgcgtagc
     2521 gcggttccag ttcgatggct ttatgtaaga cctcgatggc tttttgcgtt tccttttttt
     2581 ctacaaacgc taaacccaga ttgcgatagg actcgaccat ttgcgggttg atggttatgg
     2641 cattttcaaa ctcggcaatg gcctcatcca ccaaaccttt gtgcaaaaag agcaagccga
     2701 tcttattgct ggagacggcg tgtttgatgg ttttgatgcg atcacggata aaaaaaagca
     2761 actcgatttc ccacgcgata tcctgatgaa atgccgctac gacatcgcga aaacgatcgg
     2821 tatctaaata tttatactgt tccgggtcga gttttttttg atgaacggtg acgatacggc
     2881 cggaatcaaa aatttccgct ttgaccaaaa gggaggataa attaccggct gtttgtatat
     2941 gaaaaacttt tccgcctgca tatacgttgc tattgacgcc tattgattcc atagacacct
     3001 ctgctaataa catttacata cgattttaac atagtgaaaa cctgaccact acggtatgat
     3061 attaggcagc agggcagcgc atacacaata ccctaagcag ggtatattga aaacctgata
     3121 aaaagggtaa aatgatgaaa ttatatggaa gctttttcgg agaggcgtgt cttgattttt
     3181 tcctgaaatg caagaagtgc tgcggcgtcg aagatctgaa ttttgagacc tgcgatgata
     3241 cctacgccgt caaagacggg ctgataaacg atggtcactt cctgttgccc atacgcagcg
     3301 tttcgttgta tcggttgtcc gtcaagtgcg cgataaaacg cttcctgcat ggggagatct
     3361 tgttcgaata gtttgaacat gctttggcct tgccattcgc tgcggtacga gggcagcatc
     3421 agtgtgcggt ccccttcgat ctgcgtgatg acgccataag cgtcgcagtc aaaggtaaag
     3481 agcggttgcg gcgcagagcg catatcttcg gccgcttcgg ctaaaagcat atcttcgatg
     3541 gaataaccta cgcataaaat cgtcagcgct ccgccgatga tgcgcgtctc catgtgggtg
     3601 gcttgtccgt caataaggaa acgaaaggcg gtatgaacgg gatgttccgg taaagcctct
     3661 tgtatacggc tttcaaaagc cggttgatca tcggggtgaa taagctgacg gatatcataa
     3721 ccgatcgtcg tgtaacgcgg tttattaaaa cgtttttcaa aagcggtatt cacagactga
     3781 atggccattt cccggttgat cgtaaaagcc cactgcggga tttcttccag tgcacccttg
     3841 agtgcgattt caggaacagg tacttcctgc atcacaggga tgatttccgg cggcgctttg
     3901 aaggcaatga tacgcgatag atcgtcgcct tcggtcaacg acgacgccga cacttcaaat
     3961 ttttgccctt ccatataacg ttcgttgcgc acgagtatgg taaaaacttc accggagcgg
     4021 gcgcgttcga gcagtttttg attgtcaatg ggttgctcgc tgccttccat aaatacgccg
     4081 tgaatgcccg accacaggct gagcgacgtt ggggtcccgg ccgttgtcat gatatcgcgc
     4141 gccacatcgt tttgaaaagg aaactggccc gccgcgtcca cgatcatcgc accgtcgtgc
     4201 gccgtttgta cgatggtttc gatcatacga aactgacgtt cgatcgtgtc ctgaagttct
     4261 ttgttttttc gatcggctgt ttcttgcgaa cgtttttgtt tggtgacttc gtgaatatgg
     4321 atggccgtgc cgttgacttt gttattgacc aaaaccgggt taaatgacac ttcggcgagc
     4381 acaagccggt cgggtaattg aaactgctgt tcgatcataa atttttcacc gcgcatcgca
     4441 cgcgtaagta cgatcaccca tgccgcctgg gcatccggct taagatgatc gatcatattg
     4501 gaaccgggat gcagttcgat ctgatggcgg agatgaaaca gccgtttaaa cggctcgttg
     4561 atcagcagta ctttgaaatg ttcatcgacc gtacacaccc catccggcgt attgcctaaa
     4621 atgccggata cattttcatt gagccggtac aaaagtaacg cgagcttttt attgcgttgc
     4681 agactgtaac ggtaaaaaac ggcgataacg ctgatcagaa tcgtgatcgc gctggcgatg
     4741 atgagaaatt tgatcgccgt gtgccagtcc tgactaaggg attcctgata acggatacta
     4801 cgcgtgcgaa gtttttcagc ggagataccg agcgattcgg cggcacgatg aatttcgtca
     4861 atcatttcgc cggtgaggtt ttctacccgg atgctatcgg gaatgatcga ggtgcgtttg
     4921 aatttacgtt tatgaatacg ctggtagaga ttgtcaatac gtggcaatgc attgcgcata
     4981 tcaaataccg acccatccgt ccaatctacg ccgagcgagg cttgccaggc tttttgatag
     5041 atacggttcc agtcatcaaa actcgtgtcg gtggccgcta aaaattgatc ggaaataact
     5101 tcttcaagac cgtacacggc ttgttgcagt ttatatatac gggcgatttt atcggtatgc
     5161 gattgtattt gcgtctgatt ttccgtcatg gagcgcatga ggaaaaaaca ccaaacgatg
     5221 aaaggaatag gaaacagcgc gacaaacgcc ataaataacc ggttacggac ttgctccgtc
     5281 atgccatatc tccatctgag cgcgggagtc ggatcgtcgg ggtgcgcgtt taaattttga
     5341 ttcggtcgga atcaaaagta acgaagaaac ttgtttttca aaagtaaaat ctttagttat
     5401 gcgtaattat tttaacgaaa ggaaaacgtt tggatacgca cgaaacatgg atggcgatgg
     5461 cgtttaaaga agcggaaaaa gcgtacgcta aaaatgaaat tcccgtcggc gccgtgattg
     5521 tcttggaaaa ccgcattatc ggacgcggtc acaatcaggt agagatgctt caggatccga
     5581 cggcgcatgc agaaatgatc gcgatgacgt cggccgtggc tacgatcggt tccaaatggt
     5641 tgtacggcgc tacgatgtac gttacgctcg aaccgtgctc catgtgtgcc ggtgcgctgg
     5701 tgctgtcgcg aatgagtcag cttgtttttg gagcgggcga tcccaaaacc ggcgcctgcg
     5761 gttcgctgca taatattgtg caggatgaac gtctcaatca caggatcgaa gtccttcgcg
     5821 gggttatgga tgcgaaatgt tcggccatac tcaaggattt ttttgccaag ctacgcaaac
     5881 ccgccccaca ggataattaa gcaaacccac gtcgtttttt tctgcatgaa cccaatccca
     5941 tactcatggg gttgtgccct ccgacacttg gctgtatctt acagcacgca agttttcatg
     6001 tcttcaaaaa taattctaat ggttctcatt tcgtgaagga ggtcgtatga caattcgaag
     6061 cgtgctctta tggtgttgtt ttgttctgtg tttacccgca gtgcaagcgc aaaaagtcaa
     6121 actcaaaacc ggttcggtca aagaaaaaaa agacgaaaag aaggaaaaca aaactgaaac
     6181 gacatcatcc ggtacgttca aagtgggcga tgtagccgaa ggcttggaaa cggatggtaa
     6241 atggtataaa gtggatattc ttaaagttga agccgataaa tattttattc actggcaagg
     6301 ctataagtca acgtatgatc gctggattga agccggtaag gtgagggcaa tcggttcgtc
     6361 cggaagtgcc tccgctaacg cgagtacgtc cgcggcttct accgcttcgg gaggtaaaac
     6421 caaggtgtac ggtaccggag acggaggatt caaagtaggc gaatatgcgg aagtgctcga
     6481 ccgtgacgga aagtggtatg aagcggaaat tcaaaaagtg aatgaaggtc aaccgaacga
     6541 gtatttcgtg aaaatcgaaa actataacgg attttgggtg aaagaatcca acgtgcgggc
     6601 gaaaagctcc ggtacatcca gcgagcctgt cggatttaag gttggcgacg tcatcgaggc
     6661 cgtggatttt ggtcgatgga ataaaggaac cgtccaaagt attcgcacca ccaacagcgg
     6721 gaataagatg tttacggtgg cctttgataa cggaggctct aaagaattta cagaaaataa
     6781 actccgcgtc ataccggtgt ataatttcag cgggtccaat tataaaacgg gtcaatatat
     6841 tcgtatatgg tacagcggca gagattcgtt ggacgcgccg atcgtggaca tcaaagataa
     6901 caaattactc atcaactacg gcggatatgg cgccaagtgg tttgattttg atgcaaaaga
     6961 aattagaaat gatgcgcgta ccgcggccat cgcggcatcc aaagccacgg aagaacagtt
     7021 ttttcaagat tgtaacaaat attccgaagc gataaggata ttcggttatc tttacaataa
     7081 aaaacatgac acgcggtttg tactgtacaa tcagggtttg acttcaccgc gtccctccga
     7141 cgtcgggccg atgctcaaac agatgaatga actggatgcg ctcatcaaaa gcaaataccc
     7201 taatattcag aataccgaac aaacatggac tacggaactg tccaaaaatc ccggacattg
     7261 gcgcgtgatc gccgaagagc gaaatgaatt ggctcagagc gtcgtgacgg atatgatcaa
     7321 agacaaaatc agtgaagcga cccgaatttt gactaattat gaattcctga aaaatcatga
     7381 aatcggtgaa tttgaagcag gtaaaggaat cggcgttaca tcaagccttc aggatatttt
     7441 ggataataac ggcgccgatt ttatgaaaaa ggaagaagaa aaaatagccg atgttatcaa
     7501 agcggcgcag gaagctaatc tcggtcaaaa tgaaataacg gcgtttcggg aattcctaaa
     7561 aaaattatcc gtcgatgtga agagcaaatt cccttcgtcc ctcgtatcgt cgcaatttaa
     7621 tccggcattg catgatgcgg ctgcagaggc ctcggctaaa gcgtatatcc aaaaaactta
     7681 tagcggcgcg gctattttga aaatcggtgt tatcggtaat caggttatcg aaaaaaataa
     7741 tctgggaata ccgctgtacc gctataaaag agtcggtgta ctattcagag attcccaagg
     7801 tcgttgccgt tttgtcccgg cgtattacag agaagactat gcgggcggag gagcgtatgg
     7861 tacgccctac atcgagcatc actttacgta ctaccaaagc tgcaattgag tatttctgaa
     7921 aattattttc aaaaaaacaa aagacctgct ctttcaagca ggtcttttta ttggattcaa
     7981 ttatatagcg cagcgcgtga aatttttatt tgccttcgcg tttcattttt tcagaaaagt
     8041 attcgatcgt ttttagcaat ccggtttcga tatctacggt aggctcccag ttcaaaagcc
     8101 gtttggcttt gctgatatcc gggcggcgaa ccgtcggatc gtctttaggt aaaggacgat
     8161 atacgatttc acttttgcta ttggtcagtc ggatgataag ctgagcaaac tgaagcacgg
     8221 tcatttcatg tggatttccg atatttacag gatagtgttc gctggattga ctcaggcgat
     8281 agatgccgtc aatcagatcg ctgcaatagc agaaagagcg cgtttgtttc ccttcgccga
     8341 aaaccgtaat gggttggttg cgaatggctt gattgataaa cgtcggcacg acgcgcccgt
     8401 cattagggcg attgcgcggc ccgtaggtat taaaaatccg tacgatgcgc gtatcaacgt
     8461 tatgaaatgt atgataggcc atcgtcatcg cttcggcaaa tcgtttggct tcgtcatata
     8521 cgccgcgcgg gcctaccgga ttgacgttgc cccagtactc ttcatgttgc ggattgatca
     8581 acggatcgcc atatgtttcc gaagtcgagg cgagcaaaaa accggcgcct ttgcttttgg
     8641 ccaaacccag cgctttgtgt gtcccgagcg agcctacttt gagcgtctga atgggtaatt
     8701 ctaaataatc aatcgggctt gcggggcttg caaaatggta cacgaaatcc acagggcctt
     8761 cgacgtaaat atagttggtc acatcataat ggataaacgt gaatcgttta tcgcggatgt
     8821 gcgaaatatt atccacgctg ccggtgatca gattgtcaat ggcgatgact tcatgtcctt
     8881 cggccaaaag ccggtcgcaa agatgggagc ctaaaaaacc ggcgccgccg gtaacgacta
     8941 ttcgcatagc ggtgatccta attaaggttt aaccattttt taaaaatttg ttcttgttgt
     9001 gccgtcggct cacttacgcg ctctaaacga taccgcgccg gcatttcgga gggcgtgatg
     9061 acgatttcct gaagttgggt atcccgtgct attaatatgc ggacggaggt tccgatcttc
     9121 ttttcattca gacgagcttg caaagttcgc gcggttacac gaataccgtc cacggcgagc
     9181 acttcatcgt tcacattgag tcctgcatgc catgccggag catctctaac aacggtacgt
     9241 atcgtaaatt caccgttatt tcgcgtttca aaaccataat acggggcatc ggatttgatt
     9301 tcttttacgc gcaagccgac cgtttgtaat gccgcattat aatctaattc ggccgtaccg
     9361 cgaacgtagt tttgaaaaaa cacgtcgagg gatgttccgg ccaaacgttc ggcggctttt
     9421 tgaaattctt ccggtgtaaa accacgcccg agtttctgat cgtattcagt ccaaagccaa
     9481 cgcagcaaat catccaaact tttttcgccg cgcgtggcac gcaggatttc catatccagc
     9541 atcaggccga gcaaggcgcc tttggtgtaa tatgaaatgg acgaattcac cgaattttca
     9601 tccggcatgt aatatttgat ccatgcatcc caactggctt cttcgacact gcgaacaaaa
     9661 cgtccgggtg cggattggac ttcctgaata ttaccttcga tgcgacgcaa atactgatct
     9721 acggtataat atcccgcacg ccgtgtgatc agatcatcat agtatgcggt aaatccttcg
     9781 gcaatccata acagtttagt gtaattttct gacgtataat caaacgggcc gagttctttc
     9841 gggcggatac gtttgacatt ccaaagatga aaatattcat gcgcggtgag cgacataaag
     9901 ctattataac ctgccgcatc ggcaaaagac cacgaatcca tgccgagcgt cgtggaattg
     9961 gcatgctcca gtccgccgcc ataaccgggt acgcagtgaa tgatgaacac ataccgttcg
    10021 aaaggcagtt taccgaacat ggctgcggtt gtttcagcaa tttttcgcat gtctcgaacc
    10081 atcgcttcgg tatcgtagtt accgatgccg tagatggcaa tttcatgcgg cacgccttgc
    10141 aattcaaaat ttaaaatttt ttggttaccc atttcgatgg gggaatccac aaggacatca
    10201 taatgatccg cgacaaatcg gtgagtactg ccggataccg gtttgaggcc ggtcgaaata
    10261 tgcttccact cgttgtaaag ttctatcgtc acggtatggg tcagactttg atatccgtcg
    10321 gcatacataa aaacactggc gccattgaca taaccgtgcg aggcatccat atagcttgtg
    10381 cggacgctga gttcgtaggc atatacagca taatttacgg cgacattttc cgttttaccg
    10441 gaaaacacgc gccatgtgtt tttggaaatt ttttcaacgc gcagagattg cgaacccgac
    10501 gtggcgcgca cattttccac gttccttgaa aactcacgca ctttgtatga accgggcgtc
    10561 catacgggca taataatgtc aatgtaatct ttttttatgc cttgcatcgt catcgtcact
    10621 tcgaaatagt gcgtatgcgg cgcgggcatg cgcaaggtgt attcgatgga gggtttattc
    10681 atgcttgcac gtccttcgct cgcaagcccg gccaggagca ggcctgcgca cataacgatc
    10741 cgataattca tgccgtcgtt ccttcatgaa aatagttagg ttcatttcag atttgtttgc
    10801 ggagacgccc cgaggggatc atcatttgtt cgcggtactt ggtgatcgtg cgccgtgcta
    10861 tcggcgtacc gcgttcggta aggaccttag ccaattcttc atcgctgtaa ggatggcgtt
    10921 tgtcctcctg atcaataacc tctttcaata aagttttgat ctgtttggtg gagacgtctt
    10981 caccgtcgct gttggtcatg gccgtggaga agaaatattt taattcgaat acgccgtaat
    11041 cggtctggac gtatttaccg ttggtcgctc ggctgatcgt cgaaatatcc ataccgatac
    11101 gtacggccac atcttcaagt ttcatcggtt tgatgtggtg gacgcctttt tcaaaaaact
    11161 cctgctgcag ttcgacgatg gactcgatcg tacgatagat cgtatcccgg cgacggtaga
    11221 gcgaattgat aagccacttt gcggcttcga gtttattctt gatgaagtct ttggtttctt
    11281 tgcccgtttt attgcgctcc agcatcatcc gtttgtaagc attattgata cgcagatggg
    11341 gcacatcgta atcgttgagt atgatttcaa atttgccgtc gatttttttg acggcggcat
    11401 cgggcgtgat atagttttgt tccggcgtaa tatatccttc gccgggtttg ggattgatcg
    11461 aaagtacttc gctgatgctg tgtttgatgg cgtctatggg aatatttagc attttagcga
    11521 ctttatcgta ccggcgattg ataaaatctt cataggcttc atgtatgatg cgtatagtga
    11581 tatccgtgcc gttttgataa aatccatccg attctttacg ccgatacagc tggatcagaa
    11641 tgcactcttt gagatggcgc gcaccgatgc caggcggatc aaacgtatgg atggttttta
    11701 atattttttc gacggcttcg gccgtggttt gaaattcatc cgcgacgaat tgtaccgcat
    11761 caattttagg tgttgttttt tttctatgtg tcgttttgcg ttcagattct tccgcgggtt
    11821 cgtccggttt atcggccagc aacgtatcgt cgcgaaattc accaacggct tcggtaaagc
    11881 gctgcacttc atcggcgatc ggtgtttcgc cggtgagcgg atcatccatc acaaaatatc
    11941 cgtcttcatt cagattccag atgatatatt ccgcgatacg gcgttgttct tcagtcagcg
    12001 aagtccaccg cagttgttcg agtaaatgat cgctgagcga tactttgtcc ggttggatcc
    12061 attcctgatc atcgtcgtct tcgccctggc cgcggggcat tcggatctca aacgaactgt
    12121 cgtcgttgag gattttggcc caatcgactt cttcttcctc ttttttctct ttgtcttttt
    12181 ctgcctggat agcaacttct tcctctttgg ccacgtcttc gggtttttct tgctcctgtt
    12241 tcagttcagg tttttcttgc gtctgttcga gttccatttc ctcgtcttgc agttcttcga
    12301 gttcctgcga ttcttcgagc agcgggtttt gctctaactc ctgtttgagc cgttgttcca
    12361 acgcggtgat aggtaactgc agcaagctgg atagcatcac ctgttgcggt gacagcttca
    12421 tttgctggct gagtatttgt ttcatcccct gtgccataat cctgatctaa tccctcaatt
    12481 tgaatttatc gccgagatac aatttacgcg cttcggggtc gtcggccaga aattgtgccg
    12541 ttccgctttt gagtacgcgt ccgtcaaata gcaggtatgc gcggtcggtg atggccaagg
    12601 tttcgtgcac gttgtgatcc gtgatgagaa taccgatgcc gcgtgatttt aacccttgca
    12661 cgatggactg gatgtcctcg acggcgatcg gatcaatgcc ggcaaaaggt tcgtccagaa
    12721 gaagaaagcg cggattggtg accaaggcac gtgcgatttc ggtgcgccgc cgttcgccgc
    12781 cggaaagtgc ataaccttta ctcttgcgaa tgtgcgtgat gcgcatgtct tccagcagtt
    12841 gatcaagccg ctcgcgccgg gctttgcgtt tcaagggcag cgtctcgagg atggccatga
    12901 tattatcttc gacggagagc tttcggaaaa tggaagcttc ctgcgcaagg tagccgacgc
    12961 ctaattgtgc acggcggtac atggcgatcg tggttatctt tttttcatca agaaagaccg
    13021 agccgtgatt aggcttgatc atacccgtaa tcatgtaaaa cgtagtggtt tttccggcgc
    13081 cattaggacc gagaagtccg acgatttcac cctgcgtgac ctgtacgttc acgccattga
    13141 cgacggtgcg gctgccgtag cgtttgacca atccctcggc acgcaatgtt tttttctgat
    13201 ccgattggtg atcaatatag tcggccaatg ccggtaaact gggttgttcg tgttgtacgg
    13261 tttcgctcat ggtttttcga ttcgattctt caggttttcg ggaaaatagg tgccttcggc
    13321 tccgccatcc acgcgaaaat tacgtaattt tcctttggta tcaaaaaaca aactcatgtt
    13381 aggaccggat atgcgattgg cgccctcccg gccttcgaga taatatacat tatacgcatt
    13441 tccctgcgct tccatcacat ccaggtgttt atctttgaca tgcatggtga tttgtttggc
    13501 ttggagcgta ttgaccatcg tgcccagcgt atccaccgac gaagtggcta tggcatttcc
    13561 gatgacatac atggtttcga tcgcgcgttt gcgaaactga acgatcatcg aatcaccgac
    13621 catacggttg agtgcataat cggcaatcgg ttctttctgc agcgtcagaa attctttttt
    13681 ggtgtgataa cgcgctttgc cggatgtggt tcgaagtttt tcgcgtaaaa attccacgct
    13741 gtcgcgcacg tagacgagat ggctgtcctg atacacttcg acgaaaagcc cgcgcgccgt
    13801 gatttgttca tccggcgaaa aacgcacggg ttttccggtt acactgtcac gcggcggcgg
    13861 ctgggtatat atgccgcgac tcaggcgggc gatattttga cctttataat gggtactgtc
    13921 aaattggatc atataaggat gtccggtcgc gttcgcatat ctacgtttag catcataaaa
    13981 tccgcgttcg ccatacatgg tcatttcttc tgtgctgtcg taaatcacaa cctgaccttg
    14041 cgccgttaat tgttcgcttt gggtaaagta cacgagcgaa tcggccacca gtgtcgttgc
    14101 gcgatctacg aaaatcacat tgccttcgca tacggctctt ttttgccggg cgtaataacg
    14161 cgctttttcg cagtaaatcg tacggtggcg gtcattgaga accacgttgc cgatgaggat
    14221 cgccgactgc atttcgcgaa aaaaagcagc ctgatcgcac gtcgccgtca atgtgtcatg
    14281 cgaaaaacgt acgtcgccgc gtgccttcat cacatctttg ccgtcaatgc gctggttttc
    14341 gatttcgtcg gcctcatcca gtttgagcgg cttgtaatct tcattttgct gtgcccaaag
    14401 agaatgcacc acggcgaaga agcataaggt tatgatgaag tagcaaaacg atctcacgga
    14461 cgctccttga tcatggcatc catctcttta tcaagttccg tatcgttacg gcgtgtagtc
    14521 ttgggcttga cggtacctgt acgcagatca atatcccgtg ccgaacgacc gcttgcattt
    14581 ttcattttcc agttggtcaa atctttattg gattcgaact ggtacccgcg taacgtatcc
    14641 tcgtcggtcg tcatggtgac aagtccgtcg gcgaaaatgc gttgtttctg atgatcccaa
    14701 taaatttttt cggtttgcat ggatacgccg ctgtcggaaa cgatcacgac attgccatag
    14761 gcgaaaagat tattggtatt ggagcgaatt tcgccgctgt ccgaagtcag tgtcgcgcta
    14821 tgcgtgcctt tttcaaaaaa ctccacgcgc agtccgtttc gaaaagtcgt caattgtttg
    14881 gtatcccatt tctgcatgtg tgcaaaatgt aattctgcgt ctttcacgcc gcccgagcta
    14941 aacacggcca ccgagttcca accttcctgc ccggggcggt ccggtttttc gccggccggc
    15001 aggtcttctt tttcagtcag cgctgtacaa ctcaacaaag ttgtgtacgt tataatgaac
    15061 caatatttca taatcgtaaa tacgccaaag tttgaagcgt ccgcaattcg tcaatatgac
    15121 gttgcaagtg ctgaagtaat acctgaaata cttcctgagt actgcgttcg ctgatcgtca
    15181 gattatacac gccgcgacct cggcaccgcg ccagatgcgc catcacggcg atactttctt
    15241 tggataatgc cggtgtatcg tgtacgtgaa attcacgcgg gcattcgccg caaactaatc
    15301 ttccgcgatc ggaattgaaa cgcaggatgt ctttattctt aacacggtta tcgcaaaaat
    15361 cacagttttc aaagtccatc gtaaatccca aattatcggc catcgccatc agaaaaaaaa
    15421 gcaacccgtt tttaggacgc tgctcttcat gcagcccggc gagcgtttgc aggatcaatt
    15481 caaaaaatcc ttgatgctct tcgttgccgt gcacgacgcg gttagccatc tccaaaattg
    15541 ccatcgcgat ataggtgcgg gagagatctt tttgaatatg agtaaacccg cgaataaccg
    15601 acgcgtcgga tatataaaac atatcccgat tggcgcgttc gtaataaatg accgagatat
    15661 gattaaaaag ctccagcgct ccgccgtatt tgcttttggt gtcgcgcgct ccgcgtgcga
    15721 taagactaag tttgccgtga tctttggtca gcgctgtgac gatcttgctc gtttcgcgat
    15781 acggcgttga ccgcaatatc accgcttctg tggttattat cgccatgttt taacaacata
    15841 ctacaatgtg aattcttttg gctcgctttt tgctgaaatt aagatatttc attcacgagc
    15901 taaaagcaac gatgatatgt agtattggct caaaatttgc cactttttag aattgagtta
    15961 agtagttaag tttttattcg ttttatgaat tggcaattag cttttatttt taagtgtggt
    16021 gtcattaact gaatatttga cgcttgggtt tgtttttata tctacgtaaa cttttgtaaa
    16081 cattaagact tgcatgtatt ttagtgaata cgtattttta cgggtaacca aaatctcttc
    16141 atgggaactt taaattgatt taaaattatg cgagaaaaaa tttcattact tttttttacg
    16201 ggtgttttgt tcgccttagg attttttttg tcatccaata caaaattatc cactaaacct
    16261 cgggatggta aaaagggagc acgtattgtt tttgcatcgg aaacattaga ccttggcgta
    16321 atacgggaag gcgatatcgt agatggtgtt ttttcttttt ccaatgccgg cgatgacaca
    16381 ctcaaaattc aaaacgtcaa agcatcctgt ggttgtacgg cggctttact cagttccgat
    16441 atcataccgc cgaacggtaa gggcgaaatc aaagcaacct tcaattcacg cggcaaagtc
    16501 ggaaacattc acaaaacgat tactgttttt tccaatagcg ccgataatcc cgtcacccag
    16561 ctcaatcttg tcgtcaaggt cgaaggtaac ggctccgctc ataccggcgg gatgaaaaaa
    16621 tccaacgcca tgcatccgga agtcgaaggc gatatgcacg gttcgtattt cgaaggcaaa
    16681 tgtgctaatt gccatgtcga acccggcgtc ggaaagtttg gtaaagattt atttgacgtg
    16741 tcgtgtgcga tgtgtcacgg tgcagacggt cgcggcgccg tagcgcagcc tttgcgtggc
    16801 ggtgaatttc tcaatcgtgc ggatgcgtcg ctgctgcagg agcgtatcgc caaaggatcg
    16861 ccgtccaatg tcatgatgcg cggttttgcc aaaagcgccg gaggtccgct ggatgaaaca
    16921 cagataaaat cgcttgtaga atatatcgga agttggaaac ataagtgagt tttgtatgaa
    16981 accgatcaaa gttttagtgg ccaaacccgg tttagacggt catgaccgtg gcgccaaaat
    17041 cattgccagc gcattgcggg atgccggcat ggaagtcata tataccggtc tgcgtcaaac
    17101 gccggagatg atcgtgcagg cggcgttgca ggaagacgtg gacgttattg cgttgagcat
    17161 tctttcaggt tcgcacatga ccctctttcc taaaataatg acacaaatca aagaggccga
    17221 acttgatgat gtgttagtta tcggcggagg tatcataccg gatgaagatg ccgccgcgct
    17281 ggagaaaaca ggagttcaaa aaatattcgg tcccggcaca tccacatcgg atgtcgttga
    17341 ttttatccgt gcgtggtacg aaaaaagctc gcgccgcgtt ttggtctgag tcttttcttt
    17401 ttacggttct taaaaattaa acgatgcctt ttccgcgtct aaccaaactg caagtcaaag
    17461 aatattccga agtgctcgac aaacgcaccc ggaaacaaaa ggcattattt cacatcgaag
    17521 gtgtcaggtt atttgaggaa ttttcatcct ccggatggga agccgaatgg atcgccgtag
    17581 acacaccctt tttggaagct catccgcagg ctgcggaatt tctgaagaag aaatttggcg
    17641 tttgcacatg gttggcttcc agtctggata ttaaaaaact ttcggatacg gagcatgccc
    17701 aaggtatcgt cgccgctata agaaaacccg ctgcgataga cgaaaacggc agagcgcctt
    17761 tgggtaatgt tcttatcctt gatcagatcg cagatcccgg taatctggga acgatgttgc
    17821 gcagtgcgga ttggtttggc gtggaccatg tgtatttgag tccttcctgc gcggaggctt
    17881 tcagccctaa agccgtgcgg gccacaatgg gtagtatctt tcgtttgcat atagtggaaa
    17941 atgcttcact ccgtacaacg atcgaaacgt tattgaaaaa aaagtacacc gttttggcgg
    18001 cggatatgca tggcgaaaaa ataccgccga agatgctcgc accctgggcg ctgatcatcg
    18061 ggagcgaagc gcacggattg tccgatgaag ttcgatccgc gcccattcag ctgatggcca
    18121 tcgaaaaata tggccgcggt gaatcgttga atgcggcagt atcatgcggc attttgctcc
    18181 atgcgcttac acgatcataa aaacaaaccg gcttgtcatc tcagactccg tttctgagta
    18241 ttcaaaagag ctaatccgac aagcacataa ataaaaatta tggcattaac acttatcaaa
    18301 aacggcactg tgattacgat gaattccgcc cgggacatcg tcaaagccga tattctcatt
    18361 gacggcgcca tcattcgcaa gatcggaaaa attcgcgtgc cgtccggatc ccgcgtgcgg
    18421 gtcattgatg cccaaggctg tatcgttgcg cccggcttcg tacagacaca tattcacctt
    18481 tgtcagaccc tgttccgtaa tttagccgat gatctcgaac tgcttgattg gctcaagctt
    18541 aaaatctggc ctttcgaagc ggcgcataac ccggcttcca tccggctgtc ggcagagatg
    18601 gggttaacgg aattgattcg cggaggcacg acgacgatca tggatatggg cacggtgcat
    18661 catacggatc atatttttga agcaattgac cgttccggaa ttcgtgcgtt gtgcggcaaa
    18721 gcgatgatgg acgactgtcc tggtgcgccc aaagggttga tcgaaacgcg ggcatggtct
    18781 gtcgaagaga gcctgcatct catgcgatac tggcatcggc gctcggacgg gcgcctacgg
    18841 tttgccttcg cgccgcgctt tgtgctctcg tgcagtgaag aattactttg cgaagtgcgt
    18901 gacttatccg aagaatacgg cctgctggtg catacgcacg cgtctgaaaa ccgcggcgaa
    18961 ctggccgaag tgatcaaaaa atgcggcacg cccaatgtcg aatattttca tgatatacgc
    19021 ctcaccggcg agcggctgtg ccttgcgcat tgcattcacc tcaatgaaac agaacgccat
    19081 atcctgcgtg ataccggcac caaagtgatg cattgccctt cgtctaatct caaactcggc
    19141 tcgggcatag cccatgttcc cgaattgctg ggtcttggtg tgacggtatc cttaggcgcg
    19201 gatggcgctc cgtgcaataa taatttggat atgtttcagg aaatgcgact ggctgcactt
    19261 ttgcagaaac cgcgtctcgg cccgcgttcg atcacggcgg aagaagtgta tactatggcc
    19321 actttgggcg gtgcaaaaac attgggtttg tcggatgaaa tcggaagcct tgaaaccggc
    19381 aaacgcgccg atctgatcat catgcagttg gatgaaattc actcccgccc ggaggaaaac
    19441 ctgtattcgc aaatcgtcta ctcagccaaa agtacggatg ttcgtacggt cgtggtggat
    19501 ggaaaggtgc tgatgaaaga ccggcgcatg gtaactttgg acgatgaatc ggtgcgccac
    19561 cgcatcggac ctgaattgaa aaaactatta aatcggattg cctaaaaagt taaaaaaaac
    19621 ttaaccggaa aggtacatcg catgtataat gttcttgaag ttatcacagc ggataaaggc
    19681 attatgagcg aatattttat cctatcctgt atcgaatttt ttatcttact gatcgtatcc
    19741 gagcgggttt tagcggcccg caacggatgg gccaacccct cgatctggcg cgacaataca
    19801 cacattcgta tgtgttatct cattcgtaag tggtctatcc cttcattatt tctcatactc
    19861 aatacggtag ccgtttcgtc gtttgcacac catgctcacg aactggccga actgtttttt
    19921 atgaacgtgt tggtattaat gtcatcggcg gtgctggggc ttttgttggg agaatctgct
    19981 gtgcaacggt atcgcgtagg gcgttagttt tttaaacgta ttttgacatc ggccgcatat
    20041 acttttattc gttttccctc attttccacg accaatcctc cgtccggagc taagccgacg
    20101 gctttagctt ccatcacact atcttgctga aacacgctga ccatagtgcc aaacatacgg
    20161 cagtaggatt gccatttgat caaaatttca cggctgtctg agctgaaatt agattgtagt
    20221 gcggggatca aattttgtaa taattgaagg cgatgtacgg gtgcggcgtg agcctgctga
    20281 agcgagatcg ctgtatccga taaggcgtcg ggaaaactct cttgcgtcac attgatgccg
    20341 atgccgacga taatccatgg ttcagaaccg gttgtaaccg attccagtaa aatgccgcag
    20401 catttttttt gacaaagtaa aacatcattg ggccatttga gctcggggcg aagacgtata
    20461 gtctgttcga tggcttcagc gacagcgaca gcggctgcaa aagaataggc aaaatgttta
    20521 tccggcggca gtgagggttt gatcagtagg gacatccata aacctgcacc gggcggtgat
    20581 tcccaggtgc gtcccaggcg ccctttgccg gccgtctggg tatcggctat gacgaggcat
    20641 ccgtgttgcg ctttttcatc acgccagagt tgtttgaggt attcgttggt cgaaccgatc
    20701 gcgtccagtg tgatgatggt gtaaccggtt gtaagtgagt tgccgcgggc gagcgaccag
    20761 ttctttatgt tatcccagtc atgattcaac ggtgaatacc tgatttttta cacgaatgat
    20821 ctgcgcaaga atactgatcg cgatctcttc cggagcgtcg gcatgaattg actcaccgat
    20881 cggcgcgtaa atacgttcga ataatttgcc ttcaaatccg tcgcgcttca tgttttcaaa
    20941 aacggtgtgg atcttgcgtc ggctgccgag aaagccgatg tagtgagcac ggctgcgaat
    21001 tacgcgacgg agtgcggctt cgtccgtgat ataacctttg gtgacgatgc acacaaacgt
    21061 gtcatcgccc ggctcgggca tatcatgata gtcggccgcg acatgatgtg cgcgcacgtg
    21121 cggcaagtca tgcggcgtac gtgcgtattc ggcacgatct tcgaaaagac tgatagtgaa
    21181 atgcagtggc gccgcggcgc gcatcagagc ttgcccgata tggccgccgc cgataatcca
    21241 aagttgaggc gtattgtgca tcgcttcgat taaaacctcc acacttccgc cgcacacggc
    21301 gccgatggca tgctgaccgt cgagcgtgaa cgcatacgtg cgggttaagg gtttgccgga
    21361 ttgtaaaacc tctaaagcgt ctttgattac cagagattcg aaaagtccgc cgcctatagt
    21421 aaactgtgcc gtgccatccg gaaaaatgag cattttggcg ccggcggcgc gcggtgtaga
    21481 gccgctggcg cgtactacgg tagcgatagc gatgcggtta taccgttcta gcgctgtcag
    21541 cgcggcatgg aggaaacctt tcatgaggcg ccgctctcca tatgttcgtc gtgcacatag
    21601 cggctgctca ccagcatcat caggccgata ccgccaatga ccaagcaggt gtaaaaaata
    21661 ctgcgcatgt agtccagaaa aaatgttttt accacaccca atatgatatt ggctgtgtcg
    21721 gtgctcatag gttgtttatt aatcgccgca ggcccctcta tcttaaatat ttccgcatgg
    21781 gccgggtctt caaaatatcg gattccaaac catactccgc cacccaataa aagtcctgtg
    21841 agtatcagag gtaaagctgt gcgtacgagg agttgttttt tgttgttaaa attgatcacc
    21901 acgagtatga gcattcctgc aaaaagcagg atgatactgc ctaagcggac attgcgcatc
    21961 gtagtgcccg cttcaataaa gtccttatgc tgcattttcg aatttttttc atccatccac
    22021 tgtaaatagt agatcgtatc cggtaacgcc gtgagtcctt gtgaaacttc ctggcgtatg
    22081 atagtgcgca cctccgaggc aagtgactgc ggcggtttac agtccagttt tattttatcc
    22141 agagtaattt tgccgttatt ccgcatatac tgtccggcta aagtaagggt ttgccggggt
    22201 gaacattccg gcaaagcatt catcttgttt tcgatgattt gtgccagatt ttcgagcaac
    22261 aaatcttttt tatcggtgat gatgatgtaa ccgacatgat cattttggga aggttctgca
    22321 ttgagcgggt tgatcagatc aagatgcgcc gtgcggaggg acgtgtaaaa ccaatcctcg
    22381 gcaaaggttt tagtcatcag ccgctgtact tcctccgtgc ttaacgccag cgccgtggat
    22441 tcgagttttt taaactgccg atgctgcgtc acattacgtg tagcctgttg tatcagttta
    22501 ccgtacaaac cggattgacg tacggtttgt tcgatactgc ccgtatccat gatataatga
    22561 aaaaaccgtt cgacggggat caacgcgcat gcggctatta ttatacacat ggcgatcacg
    22621 tatgaaaaaa aggaacgcat aactaccggc cttgtaaatt gggataatca aaatcaaacc
    22681 gcgttttgaa ataccaacgc gtacatacaa aaagaaagat caacaggata atataaaaag
    22741 gaaaatacat tgcgaccaaa gaagtaaaaa aaaatatgct gaatttcaaa gcacgaattg
    22801 catcggcttt tgttttacga atgaccataa aaacaaacat cgggatgacg gataccaaaa
    22861 cgacgagcag cacgtattgg tccagaatga aagccgtgat agcggctata caaaaaagaa
    22921 caaaagccgt atacatacta aacgtaacgc cgtaacgcac ggtgaatgtt tttttggatg
    22981 tggccgcatc gccttccgtg tcggggatca tcgtcagaac acaaagcgct ccattgaaaa
    23041 taaaataggg aaatcccgtt atcaaaaata aaagccagga ttgatcgctc agcgcccaac
    23101 cgtaagccaa tgcaaaaaca cccataccgg cattcgctaa aagtcccgtc acggcccgat
    23161 ctttgagacg aaacggaggg agattgtata aaatcgcaat caaaagccag ccgaaaaaat
    23221 gaacgatcag catgcgttca ccccaaaatg aagccatcac taaacaaccg gcggataata
    23281 aaatggattg taaaataagt atgcggggat ttactgaacc gtcgtaaaga aaaaaaagtt
    23341 ttttattaat acgatcggtt tcgatatcgc gcagctgatt taccaaaaaa ccgctgccca
    23401 tcaacatggc cgtggaaata aagagtccca tcatatccgg ataccataat ccgttccata
    23461 ccggccaatg cttcgcttga cctgtagccg ccatatatcc tgctaatgtc gtagtccaac
    23521 cgggaatgaa taaaataggt cggagaatga aaaaataatc aaggcgtcga agtgttttac
    23581 tcgaggatac aggcattttt gaagggattt tttaagaaga gtatagaaaa aaagaaacga
    23641 atgtgcaaaa tcaaaagagg gaaatatccg gaattcagag taagaactat tctctgaaaa
    23701 acatctggat aaactataga taaagaacgg gaaaaattaa aattcgatat agcaatattg
    23761 taacggatgt aatacttcag cctttggtgc taagaatcaa aaggtaaaat gcgacaagca
    23821 acatgataaa aagcccgatt ggaaaaagaa tcccggtaat acccatcagt atcgaaggtg
    23881 cattgaatat tacggcaatc gaggcaatga tcagcgccag tatcataaat cccaaagccc
    23941 gtaaagcgac tctgcccttg ctcgccattt cgtctccttg aatgttttcc gacatgattt
    24001 ccatagtatg catcgtctaa ttcttgtaca aagttattgt tttttgctca gtttatttat
    24061 taacaaatgt tatgccgtat ttttcggtaa gcataagttg ttttatttca aattcttagg
    24121 attgtttaca taaaggtgta gtatataagt taatgtatca tggcagaggg actgtctcat
    24181 gctggagcaa tagctggttt aagctgtgat tgaaaaagga atgggttatt ggtgcttttt
    24241 tggtgcgagt cggctcgaat acgatgcttt cggtcaactc gtcaagcgaa cttgcttcat
    24301 tacagctcag ccttgctttt ttgcatgccc gttcttaggt tggcaatatg ggagacgcca
    24361 aactgcatca attagacttt catctgcatc atcttcctcg gctcaaccgt acggaagcgc
    24421 aggctacgct tgttccaacc gacgcggtcg agtccgtgca agccatcttt ttatcggtgt
    24481 atcgtacata caagtcgcag agccgtcgat ggatttcggt gacgcgcatc gaagtgtcat
    24541 tttacaacta cacacagttt aaacatacga tgcagatccg ggatcatacg gcgcgcgtgc
    24601 gtatcagcgc cagtgcgcgt acggaaccgg cgcaattttt tgaagcattg gcccacgtgc
    24661 tgtggtccaa aattttcggt gtgcgatgtc cggatgcgat catgcagtat tacaaagatg
    24721 cggaatggcg tctgagccgg gaaaaggaaa ctgaaactaa gcctacgatc aaacgttttg
    24781 ccccgccaaa aggacgggtg tacgatctcg cgcagatcaa agatcaatta gatgccgtgt
    24841 attttgatcg gagcatggcg acggtgcaga tcggatggag tttgcaaaaa gcacggacac
    24901 gcctagggca ctatgaccga tcggcacaga tgatcattat gagtccggta ctggatcatc
    24961 cggacgttcc gccggctgtc gtcacggcga tcgtatatca tgaaatgctg catcacaagc
    25021 accccgtcgt atatgacgaa gggcggcaga tcattcattc gcgcgcattt cagcaggaag
    25081 aaaaacgctt tgtgcactac gcagaagcac agcactggct caaaacggaa tatcctcaat
    25141 ttctgacgcg tcgccgcggc ccgcgttggt tcaaatcctg gtttacgtaa attcgtatgg
    25201 ccaaaaaact actgatcacc ggcggtacgg gtacgctcgg aagccatttg gtacgtcaag
    25261 ctatcgcctc cggcgcgtgg gatgaagtcc atgcgaccaa ctattcccag cgtcccaatt
    25321 ttcacaaggt tttttggtac ccgatggatg ctcgccaacc cgtcggcgcg atcattgagc
    25381 gcatcgcgcc gacccacatt cttcatacac tggcattgtc atcgccggat atttgtgaaa
    25441 aaaataaatt ggaagcctgg caggtcaatg tatccgttac gcaaagtctg gcggaatatg
    25501 ctgcgcaaaa caaagcacga ctgatctaca cttcgaccga tctggtgttt gacgggacgc
    25561 aagggaatta cgcggaaaac gatacaccgc agccgctcaa tttttatgcg gacagcaaac
    25621 tggagggcga acaggccgtt cgcgaagtga tgaaagacgg tcattttgtg attgtgcggt
    25681 tagccaatct gtacggtccc aatctcaacg gcaaagaaaa tttttttaca caaatgacca
    25741 aggcattgcg tcagtcatcg gaattgaaat tgttcaccga tcaaatccgt tcggtgatgt
    25801 cggcctccaa tgcggcggct tgtctgttgg aactggcgga tttatcgttt gaagggcttt
    25861 tgcacttagg cggtccgcag gcggtgagcc gttatgagtt tggtcaaata ctggcgcatg
    25921 cgatgcaggt taaaaatccc agcattaaaa aaataacaat ggcacagatg cccggcggcg
    25981 ccaaacgccc ggctaatgtg tcgctgaaaa taacgcaagc gcaaaacata ttaacgacga
    26041 agctgctcgc accggaagaa gggcttaagc gggagatcga agggtattga taaacctgcg
    26101 actagcgaag atgtttggct ttcaacgcca tatcttcaat ttttttgtgg agcagtttgc
    26161 tggcttctgt tttatccgtg ccgctcagat tttcgatcaa gcttttatag ctgcgcagga
    26221 cattggcgac tacatttttc gcctcgatgc gctcacgttc ttccgaactc ggatcggtcg
    26281 ccacttccag tgttgtcagg gcttcctgga tttcacgtgc cagcgttaca cattgtgttt
    26341 taaattcgtc cgtcatagcc gtttcatttc tcatgaattt tgataaattt agccacagcg
    26401 ccgcgcattg tcaattcgga attatttttg ttgcgcggaa aaccgttcgg attttgccgg
    26461 cgcaccataa aacgaaatac cgcttttgaa atcattatta tccgccatcc ggctccaaca
    26521 tttttatggg atcgcgcttc atcgcacggg gggggacatc tgggaagatg cgatcacgca
    26581 cgtcgccgca gcgcaattta tcgatggaaa aatcaccgtc gtattggacg aagcctgcgc
    26641 ttccgatgat acgcgcgaag cggtgttacg ccgtttgcaa gatacgatcg gggatgtgcc
    26701 gttggtttta ttttcgtcgg cggacataga gtttttgcac gcgtcgttcc acgcgatgta
    26761 tgataataaa gatcgtgctt cgccttttct gatgaatgaa tgctgggatt tgcagcggct
    26821 ggcttcaaca cttttgccgg tgatctcatt tgccacgctc gaagaattgg cggtgtactt
    26881 tacttttccc gaaggtcaaa accccgcgcc gagcgaagcc gtcatattga tcttttaccg
    26941 gttgatcgaa tcgcttgcgc gtatggagcc gcgtatcgtg tcacaggcca atcgcttttt
    27001 ggagcacagt aaaagtccgc tgaaacgcat tctgcgcggt ttggcggact atattcggga
    27061 tggtaaaaac attcatttca ttttccccga agtgcagtgg ccgacaccgg cacccaatgt
    27121 aatcggggtg gccgacgggg gctttttttc cgatacgatc cattcggttc ccgacagcga
    27181 catacgccgt tttttctcac cgcaaggcgg actgagccgt agtatggaaa cgtacgaaga
    27241 gcgcgccgaa caaacgcgcc tcacggttgc gatcaattcc gcattcaacg cgtcgcaaca
    27301 tattcttgca gaggcgggta ctggtaccgg caaatcgctg gcgtatctga tcccggcgat
    27361 ccactgggcg tcatacaata acgctttggg tgaacaagtc gttatcagta cgcacaccaa
    27421 aaatcttcag gatcaacttt tttacaaaga cattccgctt ctggctcgta cgctgccatt
    27481 cggattccgc gccgtgttgc tcaaaggccg gagcaattat atctgtatga aaaaatggac
    27541 gcacatcacg gccgatgccg gcagtcatgt acagccgcag gaacgtgaaa aattattacc
    27601 gattttgttt tggattcata aaacccggac gggagacatc agcgaatgcg taggatttca
    27661 ggccgaggcc aatcccggcg tgtggcacaa actggcttcc gaaagcgcat attgtcaggc
    27721 gcagcgctgc agccgttcag aagattgttt tgtacgtaaa atccgcgaag aagccaaaca
    27781 agcgcatgtc gtcgtcgtca accatgcttt gctcttttcc gatcttgttt ccgataacgc
    27841 gatattgggt gattaccata accttgtgct tgatgaagcg cataatgtcg aaaaagtggc
    27901 acaaaattat ctcggcgccg agttttcgct gtgggcggtg cgcggtctga cttccaagct
    27961 gtacgagcgc gatcagacgg aaaccggcgt attgattcag cttcggcaga agcttcagaa
    28021 aagccgtttg tcggatgtac ttcagtcggc atttttatca cagatacaga cggggacgga
    28081 tgcgtgcgta cagttttggc tcgcggcgca aacactgttt cagcggttga ccaccgaggc
    28141 gctgcgcaaa acagaatttg ccatgcaaag cgataaagcc gtgcgcggag aacgtcgcgt
    28201 gcgttacggc ccgacagaca gtttgctgtt gcgggttgcc gatgatgtgg atctgatgca
    28261 tgaaaaagca accaaactgc gtcaggcttt ggatgtattg agtgaaacga tgcgtcagtg
    28321 gccgacggat gcgatggacg gtgcagaaga aatgcgtgat cttattatac tgcgcacgga
    28381 tgaattggat gcgctgctca agacggtgga ttttctgacg gaaggcgcca acaaagatta
    28441 tgtgtattgg tacgaaatgc cacccaaaga acgttcgtac gatatgcggt tttatgccgt
    28501 gccgctcaat attgcggata ttttacgaaa cacactttta gaaaaattaa aaacctgtgt
    28561 attttcgtcg gccacattgt ccgtggcggg gcaatttaat tattacaaac gccgcatcgg
    28621 tttggatgcg atgcgctcag aaaaagtgca ggaatttacc gccgattcgc catttgacta
    28681 taaaaatcaa agccgcattc tggtgccgac atttttaccg gaaccgcaat cgccgaagta
    28741 caacgacgca tgtgccgaac tcctgaagcg cattactctc gaaacgcggc gcggtatgct
    28801 ggtgcttttc acgtcgtatc agatgatgaa cagttgttat tatcaactgc gtgatccgtt
    28861 gaatgcggca ggcattccgc ttctcatgca gggacgcgat gcgtcgcgta cacaactgac
    28921 ggagcatttt cgacgggaac gcgtgtcggt gcttttcgga acggatagtt tttgggaagg
    28981 cgtggatgtg ccggggagcg ctttggaaat tttggttatt accaagctgc cgtttgaagt
    29041 tccgtccgat ccgctggtgg cggccaagat ggagcgtatc gacgcgcaag gcgggaaatc
    29101 attttttgat tattcggtac cggaagcggt gatcaaattt cgccaggggt tcggccgatt
    29161 gattcgcagc cgctcggatc gcggcgttgc gcttgttttt gataatcgcg tggtgactaa
    29221 aagttacgga tcgtatttca tgcgttcact gccggttcag gcattgcgga tgcctacggt
    29281 agacggactc attgataata ttgataattt tttgggattt gagtttttaa atcaaaaatg
    29341 atattctgta cttaagttct atttaatgag aagaaaatga tttataaata tccattaaac
    29401 tttcaacagg tcgttaatga cttagaaaaa gattctcata atgaaccatt atttatttct
    29461 ggcgaagcat tgcttgtttt aaaatcattt cctgatagtg tcattgatat ggcgatgaca
    29521 agtcctcctt attggggtaa gagagattat caaaacgacg ggattggttt ggaagctgat
    29581 cctattcaat ttattgataa tcttctgaat gtctttaatg aactaaaacg agttctgaaa
    29641 cccacaggat ctttttggct taatataggc gatacatata gaaataaatc gctattaggt
    29701 ttaccatggc gcattgcaat aagaatgatt gacgaacagg ggtggatcat gaggaatagc
    29761 gtaatttgga ataaattgaa aggatcaatg gataattcac ccgatagatt ggcaaatgta
    29821 catgaaaatt tgtttcactt cgtaaagtct aaacatgatt atttctacga taccaatgca
    29881 attagaaaga agcctaaaaa cgttattatt aaaaacggat caataatttc tgcgaccggt
    29941 gttagtggag tccgatatag acggcaaatt gagttgtcat ctgaactttc tgaagctgag
    30001 aaaaaaaatg cgattgaaga tctcgatgta atgatgaacc aaatccgtct tggagaattg
    30061 tcggattttc ggatggttat aagaggtcat caacgtgcaa cgcactctga tgcggagtca
    30121 atttctggtc gcgctagaga gttgaaacaa aaaggttatt attttctaaa atatcatcca
    30181 aatggcagta agccagcaga tgtttgggat ataatacctg aagattctca gaaaaggaaa
    30241 attcattatg cgccatatcc tgaggatttg tgccgaatac cgattgcggc aacttgtccg
    30301 cctaatggta ttgttttaga tccgttttgt ggaacgggga ctacaatggc ggtagcaaaa
    30361 aaaatgaata ggaaaagtat cggggtggat ttgtcttctg actatattca gtatgcgaac
    30421 aaaaggtgtg aagtatggca aatggaaatg cactaagcga actatattca tataatacgg
    30481 ccgatgatga ggttaaatgg aaaaacgtaa tcgaaaaaca atattgccca tttataagta
    30541 gaaagtgttt aaaaaatcga aaatctgatg caaaacaaag tattggctca tgctcaattc
    30601 aatacggcaa agaatcagat acgattattg tttgtccatt tcgcttccta gagcggaata
    30661 aaattttttt tgattcaatt catttgctga gactacacga accgggcaat gaattgcatg
    30721 tgattaatga gttttccatt ccgggaggta atgttgatta catattagca tcagctagag
    30781 atggaaaagt taaagatttt gtcggcattg agatgcaagc tttggataca acaggaacag
    30841 tgtggccatc acgccaaaaa ttattgcaca aatttggcgt taaagttttg aaatctgacc
    30901 gtgagtccac ggatacgttt ggagtcaatt ggaagatgac agctaaaaca atcttagttc
    30961 aacttcacca taaaatccta acctttgaat cgcttcacaa gcatttgctt ttagtagttc
    31021 aagataaaat gatggattac atgacaagcc aatttgactt ttcatcgctt catgatactt
    31081 tgaacggaga ttcaatgcat tttcacatgt acgaaatgag caaaaagggt cacaaatttc
    31141 aattggtttt aaaaaacaga aaaagtactg atgctgatgg tattgcgaaa tgtttggggt
    31201 tacaggcaaa tcctcaagtt gaattaaata taattcttga aactctcact aaatctattt
    31261 ccgaaaagac cattttaaaa ttttaaaaat actcttctaa aaccgactca catcacggcc
    31321 aaaaagaaac gccttagccc aatcaaacag caccatggct ttgtttttta aactgacgag
    31381 tttggtcaga tacgcggatc tccagaaaat ccatgttgca aaaccttgcg aggtggttgc
    31441 atcaaaatcg gcaacagccc gattatctcc gatatacgcc agcatgccaa ggtgtttata
    31501 aacaaacggt tttaatgatt ccgccgattt tcctttggca agctgattga ggtagtttgc
    31561 caaatatttt ccgccttgat tagctacctg tgccgtcgcg atcaaagact tttcctccgt
    31621 cgtagcgcaa tcaccgagcg catagatatc ggggttatct ttgaccctaa aaaaagaatc
    31681 caccaaaatg cgtcccgcac ggtctttggg gcatgataaa ctttgtacga aatcggttgg
    31741 cccgttgccg gtggaccaca ccaccaaccc atgcgaaaat tgggtgccgt cgtgcaatgt
    31801 gagttcttga gccgtcactt ctttgacgga cttgcctgta agaatggtaa cacgacgacg
    31861 attgaaaagc ttgatggcgt attccgagac ggtatgatca aaagcgctca ggatttgttt
    31921 accggcttcg ataagagtga ttttagccat gccgacaaga tgtggaaatc ccttttctaa
    31981 ttcttcttcc aaaaaatcgg ccatttctgc ggcgaattcg acgccggtcg ggccaccgcc
    32041 aacgacgacg atgtgcagcc gtcgttgcat ttcggccgcg tccaaaccgg gtgtggatgc
    32101 gcgttcgaaa ttatcaatga tacgctggcg aatgcgccgg gcatcgcgaa gttctttgag
    32161 aaaataagcg tattccgcta cgccgggaat tttaaacgta ttatttacgg ctccgaccgc
    32221 gatgactaaa gtatcgtaat gcagttcaaa agtttgtgca tcggcgatat tccggcaagt
    32281 gacggtgcgc ttttcgggat ggagcgctgt ggcgccggcc tgataaaaag taacatcttt
    32341 acgcgcaagg cgtacgggtt cgatgatgct gcgaaattcg atcgtgccga cggtagtgct
    32401 cggcaacagc ggcgtaaata aaaaatgatt gcgggtactg accacggtga catcgtagag
    32461 atccgaacga atgcctttca taagactgaa tccgccgaat cccgtcccta gaataagcaa
    32521 ttgctttttc atgataggtt accgaatcgc tttgaaactg gaaatgactt gatcgagtaa
    32581 ggcttccgag gcgtcgctgt ccacacggct aaaaccgctt tcaagcatat agattttgtc
    32641 tttgtattga ccgacgccga tacggatata cgtggaatag ttatttaccg ttttgatgcc
    32701 gatcgaaaat ttgaaatcaa tattattgat tttgcgggcc tcgtcgcgga taaactctga
    32761 attgagcgag ccgactttga ggtattgcag aacaatatcg ggatcggatt ttttttcgtt
    32821 ttcagcgacc gtaataaaaa aataaatctc gctgtccggg ctgtagcatt cgataaaact
    32881 cttttccccg tccgtattga tactgatctg ccagtgcggc ggcgttttaa attcgatacg
    32941 atacggttca aaccgatgcg tttcccacga agtttgcgcg gtagccaaag gcgcgacgca
    33001 gcaaaaaata agaatagtcg ccgtgtagat tgtttttttc acaaggcttt tatcgctttc
    33061 aaaacatcct ccgcatgcgt ttcggcatga actttatcaa acacaaaggc gatgtttcct
    33121 ttttcgttga taatataagt tgtgcgtaag accccttgat acgcacgacc aaataatttt
    33181 tttggtcccc acaccccgta cattttgatc acgcggtgct ccgtatcggc caaaagcggg
    33241 aacggcaatt cgtgtttttt agtaaagttc tgatgctttt ttaccgaatc cgggctgata
    33301 ccgaaaacag cgatcttgtt ttttttaaaa gccgcgtggg catcacgaaa actgcatgct
    33361 tcgatcgtac aggtcggcgt attatcctgc gggtaaaaat agagcaccac cttttggcct
    33421 tggtaagctg aaagttttac cacatggccg tgggtatcct ctaaactaaa atcaggggcc
    33481 ttggcgtcaa gtttgagcaa tgccatgaat taatcgtttt ccgtagttac ggtccattcc
    33541 aaattatcaa aacgtgtgtc cgtgtaacgt ttcttgacga tcagaatggc aagttttttg
    33601 taatcttctt ccgacatctt ttggacatca tcaaattccg tatagctgcg gcggataaag
    33661 tcgaggttat tgcttttaaa ttcttcgttg atgccgtttt cggcgttggt cacgcgaata
    33721 gtcatgggga tactccttca aatcaatccg ttatgttatt caatactatt tttcaattcc
    33781 gcataccggc cctcgcgcag aagttcgcga tagtggcgtg catcgcggcg atgacggtgc
    33841 gcatagcggt cctgccaggg cagttcggcc gctttggtaa atgccgaatc ggcttcattc
    33901 catcgttcga tcagcaccag cgtgcgtccc aattccaaat aatgccgcgg ttcgtcccga
    33961 tgcaaacgta tcgcgcgttg aaagtgttcg atactgcgcg atatggcggc tgtgccatag
    34021 gctgttccca taagacgggc tgtcgtacgc gaaaaccacg aagcgttggc gacaaaacga
    34081 ttccacaaac cgagaagatg ttcggcttca tcggagagcg aatccagagc cagcgcacgt
    34141 tctgccgcaa aacgcatttc cgctaataaa acgctatggc gcgaaccgaa ttcgtccatt
    34201 gccgcacgtt cggccgcggt ggccaaggtc aaccacgccg ctaacgacga agaatcgcag
    34261 cttacggcga cacgcgccca ttcaaatgct tcggcgtaaa gtttttcctg ctcttcgcat
    34321 ttgggcaaat cgttgcccag tgcaatcgtc tgcatcgcca gcttccaccg ggcttcgcag
    34381 ttagccgaat ctttttgtac ggcgtggcgg tacgcgtcaa cggcttgtgc cgcattgtac
    34441 tgggcggcca gcgaatcggc gctacgcatc caggtataag gcatctgcgc ggtcagatct
    34501 gacaatatga aaaagaacat cacgacggtc atatagtacc gtcgttgggc ccataaggtt
    34561 gaggccttta tccgatctgc aaaaaaaagt tttttcatac gaatatgcct tgcgatggta
    34621 gcaagaattt aatggaaaat caagcgttgg atttgtgcgg ataaaggaca attccatttg
    34681 aattttatgg cgttttggtt tattatcaca gccctgtaaa atcatctgga tatattttga
    34741 aaaaatgaag aataataaca tggaagagac gacgtttacc gaaaaagaaa tcagcgaact
    34801 tgaaaaattt ggcatgaact cctggtttgt ggaagagttg ctggccagtt ataaaaacaa
    34861 cagcacgatg gtcaacgaat cctggcagga tttcttcgac cgtatgtctg gcaaaccggt
    34921 aacagccaaa ccggcggaaa tttctaacgg caacggatct ttgataggta acattcccat
    34981 gcccaaaccg gccgccgggg aagaggctgt tgtactgcgc ggtgtcggcg caaagatcgt
    35041 tgaaaacatg tcgtacagtt tgagccttcc gctggctacg tcgtttcgca cgatacccgt
    35101 aaaaatcctc gaagaaaacc gcgccatcat taataatttt ttaaaaaaat ccaatcgcgg
    35161 taaattatcg tttacccatt ttatcgggtg ggctatcgtc aaagcgctca aggcgcatcc
    35221 ggtgatgaat aatgcctata ccgtgatgaa cggcgaaccg catgtcatac gcaaaccgca
    35281 aatcaatctt ggccttgccg tagatcagga gaaaaaagac ggaagccgtt cattgatcgt
    35341 acctaatatc aaaaacgccg gagctatgaa ttttgttcag ttcttcgcgg cgtatgatga
    35401 cattattcgc cgggcgcgtg ccaataagat cgagccggcg gattttatgg gaacgacgat
    35461 ttcgctgacc aatcccggca ccgtaggcac ggtttcttcc aatccgcgac tcatggtcgg
    35521 acagggtgcg attatcgcca ccggtgcgat tgattttcct gcgcaatacc acggcatgcc
    35581 ggaatccaat ctttcggcga tgggcattag taaagtgatg aatgttacca gcacctacga
    35641 tcatcgcatt attcaaggcg cagaatcggg gcttttcctc aaaaaactgc atgaactttt
    35701 gcttggcgaa gacggttttt acgatgaaat ttttgaggcc ttgcaattgc ccgtcaaacc
    35761 ggcgcagtgg caggtggata attatccgat ggcgttcggt aaatcgcagg attttgaact
    35821 gatcgaaaag caagcgcgta ttttggagat ggtcaatatg tttcgcgtgc gcggccactt
    35881 attggccaat ctcgatccgc tcacgccgca gatgctgtat catccggaac tggattcgca
    35941 gacctacggt tttaccgtat gggattatga tcgtgaattt attaccggcg gcttagcggg
    36001 gaaaaagaaa gcgacactta aggaaattct cgacattttg catcaaacgt attgcgaccg
    36061 catcggcgtg gagtttcgtc acatccaaag ccctgaagaa aaggcttggc tgcaggaacg
    36121 tatggaatct tcccgcaata ccggcaaata ttccaaagaa gaaaaaaagc aaattttgaa
    36181 aaaactcatc gaagcggaga gcttcgagcg gtttttgcac accaagtttg tcggctccaa
    36241 acggttttcg cttgaaggat cggaaaccat tatcgccgtg ctggatgcga tgctgcagtg
    36301 gtgcggagac gacggtgtca aagaagtcgt gctgggtatg gcgcaccgcg gccggctcaa
    36361 tgtgctggcc aatattatcg gcaaatcgta tcaaaagatt ttttcggaat ttgaaggcca
    36421 tcacgatccg cattcggcgc aaggttccgg cgatgtgaag tatcacctcg gcgcgaccgg
    36481 taattatccc acgcgcagcg gcaaatcggt aaaaatttcc gtcgcaccca atccgagcca
    36541 tttggagttt gtcaatccgg tcgttgaagg catcgtgcgc gccaaacaaa cgcgtatgcc
    36601 cggcgaatac aataaaaacg taatggccgt tttgatacat ggtgacgcgg catttgcagg
    36661 acaaggcgtc gttgcagaaa cgctgaactt gtctcaactc aaaggatacc gcacgcgggg
    36721 aacgctgcat ctgatcatca ataatcagat cggatttacc accacgcccg aagcggcgcg
    36781 ttcatccaca tattcgtccg atgtggcgcg tatggtgcaa gcgccgattt ttcacgtcaa
    36841 tggcgacgat ccggaagcat cgctctgggt tacgcagctc gcgttcgaat accgtcagaa
    36901 gtttgaaaaa gatgtcgtga ttgacgtact cggctaccgg cgtcacggcc acaatgaagg
    36961 cgatgaaccg ggctatacgc agccggtcat gtataaaaaa atcaaagatc acgattctgt
    37021 caaagagttt tacggcgcgc gcctgatcgg cgaaaaagtc atcacggtgg acgaatttaa
    37081 agctatggaa tccgattttt accaagcgct caatacggcg ctcgatgcgg ccaaacagaa
    37141 agccatcccg taccaatcgg aaacgccgtt ggcgatttct gaagaagccg cgcaggcggc
    37201 gccgacttcg cagggaacgg ccgtatccgg tgaattgctt gcaaaggtcg tcgaaggcct
    37261 cacgcgattg ccgcacgaat ttacgttgca tcccaaattg caatcgttta tagatacgcg
    37321 caaaaaattc atgcacgacg acggcgtgac cgtagattgg gcgttcggtg aagcgttggc
    37381 gtttggcacg ttgctgatgg aaggtacgcc gattcgcctt tcgggagaag atatttcgcg
    37441 cggtacgttt agtcatcgcc atctggcgct gacggatatg aataccgatc tcgattatat
    37501 tccgatgaat tttatcgaaa tcaatcaatc caaaatcgaa gcggtggaca gtcttctgtc
    37561 ggaaacggcc gtattgggtt ttgaattcgg ctattccatt gccgatccgc ttacgcttgt
    37621 gttatgggaa gcgcaattcg gagactttag caatgtggcg cagccgatca ttgataattt
    37681 tatcgcgagc tgcaaatcca aatggagtct ttctacatcg ctggtgatgc tgctgccgca
    37741 cggctatgaa gggcagggtc ctgaacactc cagtgcacgt cttgagcgtt acctgcaact
    37801 ctgtgcggaa gataatatga tcgtttgcaa tctgacgaca ccggcgcagt atttccacgc
    37861 gttgcggcgc cagatccgcc agaaaacgga aaaaccgttg atcattatga cgcccaaaag
    37921 cctcctgcgt ttgccggatg cgcgttcata caaacacgaa tttacaaacg gcacgtttca
    37981 cgaaatatta gacgatgcga cggttacgga tcgccaagcc ataacgcgcg tgattatttc
    38041 ggccggcaaa gtgcattatg atctggtgaa attccggatt aaaaacaatc gcacggacgt
    38101 cgcgttggta cgtttggagc aaatttatcc gtacgatgcg gatacaatga aacgtatcct
    38161 cgcgtcctat cccaatgcgg ctacggtggc gtggacgcag gaagagccgc gcaatatggg
    38221 ttcgtggaat tttctgttag agcgactgac gaatgatctg acggcgaaac aaaaattagt
    38281 ttacgccgga cgcaaagcca gtgcaagccc cgccgtcggt tcgctcagcg tgcatcaggc
    38341 cgagcaggaa cagcttgtgc gcgacgcatt tgagctgtaa tgtggtttat tttttaacgt
    38401 tgttatagac agggcttgaa aatgcaggat tatcgtttca gtgaattatt tgaagcgcag
    38461 aagaagcatt ttcaaagtca tatcaagtca tcccgccccg acgcgcgtat cgtcaaactc
    38521 aaaaaaatcc gcacatggat cacagcgcac gcgcaggata ttcgcgatgc gatttacgag
    38581 gactttcgta agccgtccgt cgaagtggat ctgacagata tccgtcccgt gttgatggag
    38641 atcgagcatg ccctcgttca tattcgttcc tggatggcgt cccggtatgt atccacccct
    38701 ctgctttttt tcggtacacg atccaaagtc gtgtatgaac ccaaaggcgt atgcctgatt
    38761 ttagcgccgt ggaattttcc ttttatgttg tccgtggggc cgcttatctc cgcgatcgcg
    38821 gcgggtaatt gttgtatttt aaaaccttcc gaaatgacgc cgcaaacgtc ccggcttatt
    38881 gcccgcatga tagccgaatt atttaatccc aatgaagtgt gtgtcgtcga aggggatcat
    38941 gtggccgcgg aagcgttgtt gaaattacct tttcatcata ttttttttac cggcagtccg
    39001 gccatcggaa aaaaagtaat gcacgcggcg gcagagcatc tcagttccgt aacgctggag
    39061 cttggcggaa aaaatccggt gatcgtggac gaaacggcgg atatcgaaga tacggtttcc
    39121 aaattgttgt ggggaaaatt tatgaacggc ggccagtctt gtatggcgcc taattatgtg
    39181 tacgtacatc atacgattta cacggcattt tgcgaagcgc tggtggccat gtatgcacgt
    39241 atgtttggca cggcgaacga accctcgccg gatgtagccg gtcttgtgag cgaccgtcat
    39301 tatgaacgta tcacctcact gatccgcacg accgtggccc agggtgcgaa aacgcttgcg
    39361 ggaaatcatt tcgatgctgc gcgacgttat gtcgccccga cggtgcttgg taacgttaca
    39421 acagatcacg cggtgatgca ggatgaaatt tttggcccga tacttccgct gctgagatat
    39481 acgcatcgcg atgaagtgat cgcctatatc aacacgcagg aacgaccgct gaatttgtat
    39541 attttcagta cggatatggc aaaagctgac gcgatcatcg cacagaccac agccggttcg
    39601 acatgtatca atgaaacgac gctcaacttt gcgcatccgg atttaccgtt cggcggtgtg
    39661 aataccagcg gtatcggcaa agcgcacgga tattacggat ttctcgcttt ctccaatgag
    39721 cgcggcattc tcaaacaacg gcgcggtctg accacaatga agcttgtgta tccgccatac
    39781 acggagcgcg ttaaaaaaat cgtcaaattg gtaatgaagt atttataagt tatttctcat
    39841 ttaatctctt cgtaacgaaa acattctatt ctcataacat gcgtgttgtt tctttaaaag
    39901 aaaacaccgc gcatgaaaca aacaaaaaat ttccctctcc gcgtactcac gtttaatgtc
    39961 tggggactca aggtcggacc gcttcacatc gcacgtgata tcaaagcacg aatggatcgc
    40021 atcgcttccg ctctgcatcg atcggaatcg gatgtcattg tgttgcaaga agtatggtgc
    40081 gcatccattg ccaaattttt gattcgagag tcgggttatc cgtaccatgt ctatcagccc
    40141 gggcggggta cattgcgcgg acctttgggt aacggtcttt tgatgctttc acggtttccg
    40201 atcgtgcata cgcagacgtt gcgatttcat cattgtacgt cgccggacga atggttcgtc
    40261 ggcaaaggtg cggtagctgc ggatatacat acggcacagg gcgtcgtgcg tgtgatcaat
    40321 acgcacttag gatccggtaa aaggccgttg catgtcacga tgcgtcttca gcagctacgc
    40381 cagctccaaa gttgggtggc cgggttggaa catcatatgc caagcgtcat tgccggtgat
    40441 tttaattttt caccttcttc attagagtat tgtgtgttcc gacattgggc cacgcatcat
    40501 tttcgcgaat cgcttgcgga tacttttgcc gtgcggcacg ggcaggcgga cggccatacc
    40561 tattttgtag atcgctctta tcatgtcaaa ccgaaaatac acgatcgaaa tgaacgtata
    40621 gattatgttt ttttacttac acccaaacaa agccggcttg atctaacggt ggaggagagt
    40681 acacgtattt tgaatgatgc cgaacatccg tattcggatc attgcgctgt gttatcaacc
    40741 gtgggtatcc gaataaaacc gacgctgcgg gaaatcatgg tatccgatcc gatgattccg
    40801 gcaagaaaag cgattttatg agtgaactgc aaaccgtcat taccgtatta agcggagatt
    40861 ttgaaaccta tggttacgcc ggtttattcg gattattgat tttaacaacg atccttcctt
    40921 tgccgttgcc ggaagagctg atgtgtatca cgacgggtta tcttatcgaa cgcggtgtga
    40981 tggctccggt acctgccatt ttgacgatgt ttatgggtat cgtggcgagc gatgtgtatc
    41041 agtattattt tgctcagata ttaggaaaca aactcatgcg tgtgcggcca ttttcatgga
    41101 tactcaatga agaacgcata cgtaaagcgg aagcgctttt tttacgccag gggtctaaag
    41161 ttttattttt tggacgatgg gtgtacggat tgcgttcaaa tatacatttt gcaatgggtc
    41221 ttcttcgttt tccgattcga cgttttatcg tcatagatgc agcggttgtg agcatacagt
    41281 cgttgtcgct tgtgttgatc ggatattaca gtccgttgta tatgaaccat tgggcaagtc
    41341 accttatgac tttgcagtgg tggctctatc ccctgagtat gattgcttta agcgttgccc
    41401 tcagcggtat gattttcaaa gcatggaaaa aaagacaacg cgaagtgatt ttataaaagt
    41461 acggcgtttc ttattgagag atcgcatatt tgtattgtca ttgtcaacga aacaatagcg
    41521 tcgtaagaac acatgtaaat tcaattatga agaaattact actttttttt gcgccttagc
    41581 gtcttagcga acttgtattc agtctcgcaa agccgcaaag agaagcggga atgaagtttt
    41641 ttataaaaaa aagcccgaca ttgtatcggg cgtttttcgt ttaagattgt tgcgtgtgcg
    41701 cagcgacttc ttttttgtat cgcatataaa cccaaagtag caacccgata ccggcgacaa
    41761 aggaaataaa aaagagcgcg atgacatcgt tttgcggttt ggtttgtaat accgcatgcg
    41821 tccagttgac ttgtttcaga tattcctgac gcagcagatc gcggctgatg actttttctg
    41881 cgatacccac gagcatcgcg gcgatgccga cccatccgat catattgttc gctttgagaa
    41941 acgttacggc cgcaatagcc agtgttccga tgatgaataa tgcatgactg gaaaggaata
    42001 acatttgtcc ggcgccctcc gtgaaggctt tacgcaacgc ttccgggatg gcaaaataaa
    42061 acataaatcc gaccacggta ttggcgaccg tgccgatgag aaaccatgtc ccgccccaac
    42121 gtttcataaa cgaagaaagc gcgttgtctt ttttctcacg catgccaagt atcaaaacca
    42181 acatgcctgc tacggcgatg gccgaaaatg cgaaatgcag atagcgcggc atcagg
//
LOCUS       DAOQGF010000013        40698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_400_length_40698_cov_7.507615, whole genome shotgun sequence.
ACCESSION   DAOQGF010000013 DAOQGF010000000
VERSION     DAOQGF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 40698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_40698_cov_7.507615"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2680)
                     /locus_tag="PK937_03550"
     CDS             complement(<1..2680)
                     /locus_tag="PK937_03550"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH31677.1"
                     /translation="MKPTHLLFRRIFLTAFMIAAVVLVVVNFYRFAASPTDENIFDDA
                     PSQLYIFRSFPADISYYQKRDTTGVFIRDSVYAGDLLVSVGGILWKDQDSIARFLKRV
                     GSEEKINAIVFRSKASKMFVANFIRKDAPDSFYHEIQFTAHVREVAHNGASDRAGMLV
                     GDLIVRINGRTFKSAIEADRILSSGQIGKTIAYDILRDNQIITLDVTLAKFGIPFFLL
                     VIYGAGLVYILVGSFLVYKRADTTAAVLLGMAFFLIGMMLSLLGERGSLSTTPFGVFV
                     TNVRWWSLVLGMAMLMHSRLYFPFSVQELIQKKWVAIVPYGLSAITFIGIVVYKQALA
                     AFVSGIAMMVYYIFIRLRYKSYFSSSYRSVSRLSGWIAFACAVLGYVLTFFSDGNSGR
                     GYLFGYAGFFFALIPLAHLYTIARYHLMELNFRVRRNVQYVIASLVWNLTVVYGAVQL
                     MVIAVTSNMDLPNIRFTESSVEITDVPVSADERIQILKVVLILLAVAMTFVWVRLRSW
                     GQRWIDYKFHRSHHDYRRAAAELAEVMATNLNMNDLARGMVEKLASLMHLKMAGVVFY
                     RDEKSLCCSEVYGYESGAWQALCSKSDQPLMAAVRANTSTATINVDNLPGPLRNELRQ
                     CGFRFVIPIRSKEHTVGAIIVGERMSESPFDHDDLMFLTTVAKQASVAIENAFLYENL
                     AEQDRLKHELNIARRIQLESLPQKTPNVRGLEIAGRSVPALEVGGDYFDYLNGKPDKF
                     TVIVGDVSGKGTSAALYMSKVQGIIHSLNSFDLTPRELFIRSNRILCRDLEKKSFVTS
                     IGATFLLEEKKIRLARAGHLPLYCYNASQRKVELITPLGVGLGITDDGTFEENLEEKE
                     FAYAPGDIFVFVTDGVIEAHNIHRHEYGEERLLKLID"
     gene            complement(2854..3693)
                     /gene="uppP"
                     /locus_tag="PK937_03555"
     CDS             complement(2854..3693)
                     /gene="uppP"
                     /locus_tag="PK937_03555"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNH31678.1"
                     /translation="MDFFQAIVVGIIQGITEFLPISSTAHVRIVPALLGWPDPGAAVT
                     AVTQLGTLMAVLIFFRNDIIRLIKAAFFSVTRWSQKAQWDAELRQDVRLAWFIALGTL
                     PLGILGIIFKDTIETDFRSLTIIGWSLIGLAVILALAEVVAKHRRRIADMDFKDTQII
                     GLAQAVALIPGASRSGVTITAGLFLGLTRESAARFSFLLSIPAVLASGLFELKDLIKY
                     GLGGEELIHLFVATLVAGIVGYASIAFLIKWLQTRSTTIFIVYRIVLGILILALSGMH
                     VIR"
     gene            complement(3772..4356)
                     /locus_tag="PK937_03560"
     CDS             complement(3772..4356)
                     /locus_tag="PK937_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNH31679.1"
                     /translation="MFINWALQEINLKIVYYGPGMSGKTTNLEYIHSKMDPSLAGELV
                     SLKTKEDRTIFFDFLQLEVGRIKGKKPKFNLYTVPGQVYYASSRKVILNGVDGIVFVA
                     DSQKHRMDGNIETLLDLENNLREDGHSLDSFPWVIQYNKRDLPDVDTVENLQKRLNYF
                     NVPYYEAVGIKGVGVFDTLKAIINKVIANVQTQL"
     gene            complement(4407..4922)
                     /locus_tag="PK937_03565"
     CDS             complement(4407..4922)
                     /locus_tag="PK937_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNH31680.1"
                     /translation="MLQTKKLKDGSKQIILNGKQFEGVTKVLSELASKTKASAILFAD
                     MSGQLISQRGNTDKMNTTVLSALAASDFAATAEMAKLVGEDAKFKLLFHEGEKRNVYL
                     SNVGDNFFLVVVFDTSVTLGLIRIYTKKAIQNLIEIFEDSSNSSDDSAKDLIDSEFGS
                     LLGDALDESFK"
     gene            complement(5059..5805)
                     /locus_tag="PK937_03570"
     CDS             complement(5059..5805)
                     /locus_tag="PK937_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH31681.1"
                     /translation="MAKGLSLKWKVPLVIVSGALIAGITITILVVQLVSGSLQSELTE
                     RGKAIVTTLSKQVERPLVGDDEVDIQNLVDIASGFTAVSYVYISDKENKVVAQKFKVG
                     SYNQRTMVEAAVIDGGGDYKIDLTQVENTETYEISTPIGAGTLGNAHVGMSGKFIDDT
                     IRATALLVGGVLALAVVFGMIVALYLSNALTKQLLYLTESAEQISKGDLESIVRVDAT
                     DEIGDLADAIERLRESLKAAIERLRKKKAG"
     gene            complement(5816..6619)
                     /locus_tag="PK937_03575"
     CDS             complement(5816..6619)
                     /locus_tag="PK937_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNH31682.1"
                     /translation="MATVLSGNLSHVDVLSIVKLLVAGDRTGRILLERGDQRENGEIY
                     LANKRIVHAVCDTYLGEPAFHELILWTSGKFSFEPDVASLQKSIDKETGKLITEATLE
                     YEAWQKIARHIPSFRVKFRKTDNEPSPTIRLKAKDWELLHLLEKDEYSVDELSSRLGM
                     KDMDVAGIVYMLAEAGLITAVSGATASADKQNVSSAFFKNLENELIQLIGPVASIIID
                     DVVETFGEDQKTFPKDKVPPLVEAIANEIYDPAKQLAFKQLMLKQIRTL"
     gene            complement(6642..7079)
                     /locus_tag="PK937_03580"
     CDS             complement(6642..7079)
                     /locus_tag="PK937_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31683.1"
                     /translation="MKDKLEEINSVNGVKASFIYFNDGETVYANMPQGFDEATYVEIA
                     RDVIQVAAMFDRLSTPLHEYDLKYDNGRVIAYPMSNFNLIILCQPNVSVAMLRLTVNV
                     ALADLEADKKFQKRLSKVEASRRSFLVRSNMDAESWALVEVVN"
     gene            complement(7101..7763)
                     /locus_tag="PK937_03585"
     CDS             complement(7101..7763)
                     /locus_tag="PK937_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNH31684.1"
                     /translation="MALVGNLRDLKLTNIVQLNCMEKNEAMFTVELRNAIGRIYFAEG
                     NIVHATFGTVEGEDAVYKILAIKEGPFKVENGVASPKRTINAPWGNLMMEGLRLIDEA
                     LEAKGEAMAKFTRDIKTINGVNAVLVSSPQGEILMEDGIANGKRTSAGMGFIMRKLAK
                     IGRASKSGTYKFGIVTGKNDRKIISKWHDDIITLTVEQKAAADLIEQAIEKAPGIQKV
                     SE"
     gene            complement(7781..8155)
                     /locus_tag="PK937_03590"
     CDS             complement(7781..8155)
                     /locus_tag="PK937_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH31685.1"
                     /translation="MSGTLKRILIVDDEETLTFSLYQSFIISKENYEVVTASSGTEAW
                     EKFAEHPFDLILTDITMPGISGIELLKRVKKERPPTHVIIMTAYGSDEKKEEALSFGA
                     YRYIEKPFEIKMMKSVIAEALK"
     gene            8571..9824
                     /locus_tag="PK937_03595"
     CDS             8571..9824
                     /locus_tag="PK937_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNH31686.1"
                     /translation="MSTWLVEKCKPFSNFVEDIKAKGLYSYFTELERTGTYITVKGQN
                     LVNFSSNDYLGLTHDPRVIEEARKALDIYGSGLCGSRYVVGTTIEHTRLEEKIADWKQ
                     KENCIVFSTGYQTLLGTVSAMLVKADDSIEDDIVLAIDKLSHACILDGCALAVGTHMT
                     RGGSTKNLDGQIRYFRHNDPGSLESIVESSVKRGKNVMIIVEGVYSMDGDICRLPDFV
                     NIANKYDACIAVDDAHGTGVLGKTGRGTGEHFGMDKDIDLLMGTFSKALGGVGGFVCG
                     QRDVISHIKHKARSLIFSAASPVSNIVSVSKIIDIIRSEPEHLQNLRVNESYFKSKLK
                     EFGFNYGDSETPIVPIIIGDSEKTMYISKKLYENNILMLPMIFPAVARGQERLRCTIS
                     AKHTRQDLDHALDVLYTEGKKIGVI"
     gene            9976..11718
                     /locus_tag="PK937_03600"
     CDS             9976..11718
                     /locus_tag="PK937_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNH31687.1"
                     /translation="MTKKSVGNFILVLHTHLPYVLHHGQWPHGMEWLYEAASETYIPL
                     LNALNALQNEGRRPRLTISMTPVITEQLASETFKKEFVAFLYDEIDRARTDMVSFEKS
                     PTEKKLVPLAEYWLDFYQNTLKDFTLTYRHDLIGAFRTLQDQGVLDIITCGATHGYFP
                     LLGTDANIHAQIRMAVVTHEKYFGRKPRGIWLPECAYRPRYVWTPPVASALGSEPRLR
                     KGIEEILDANSLEYFIVDSSLVEPGRAVPMYMGRFENLRKLMMRLASENRPIPKADFL
                     QSVYDVYKTKSSYDAPGNPVSIFARDMETSMQVWSSEQGYPGGEWYLDFHKKHEQSGH
                     RYWRVTSLEADLGLKAPYEPQKIQSAIADDAQHFVQAVRQALTRFKTKHGYEGTLTAP
                     FDSELFGHWWFEGPGFLKTIFELMDDDPAIQTMHCAESLDQNPPEKIIAIPEGSWGAG
                     GNHYVWFNHEVDWMWPFIYNDEAAMRDHVQRWNNNTDPEWTAIIKQMARELFLLESSD
                     WPFLISTQSAIDYATERFMEHHTAFEKLARLSEYHCNGSALHPDDRAWLREITSQDHI
                     FDDALIDPNWFLND"
     gene            11763..12176
                     /locus_tag="PK937_03605"
     CDS             11763..12176
                     /locus_tag="PK937_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31688.1"
                     /translation="MKNSRILVLFCLSLMATAIHAQSDPFTGSYTGKGFRPGEKYTEK
                     PSYVIDVTIEKTGDYYMVRWFENGNLSYYGLGIHIDNILSVSYVSVDKSIYGTVSYKD
                     FKKDKGYLVGAWCIAPMDPEAVGNGKNGMEVLWPK"
     gene            12282..12743
                     /locus_tag="PK937_03610"
     CDS             12282..12743
                     /locus_tag="PK937_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31689.1"
                     /translation="MKKWMMIAALITAFTFSQNANAQLPVKMGPRAGVNIGDGGDYFV
                     GGHIEVSLPVLPLTFVPNAEYVFVDNATSINTGADVQYTFIGVGVAKLVAGGGYTLNY
                     NKPKGSDSATDSGFNLQFGAKASLGVSAFGLIRATNIGGNWTKAMVLGVTF"
     gene            12772..13944
                     /locus_tag="PK937_03615"
     CDS             12772..13944
                     /locus_tag="PK937_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH31690.1"
                     /translation="MAGKFKNVDYYDMDTLLSDEERMTRDTVRDFVDDKILPIIEEHN
                     RNGTFPMNLVKEMADLGVFGANLPEKYGCAGMNNVAYGLIMQELERGDSGVRSFASVQ
                     GALVMYPIYAYGSEEQKNYWLPKLAKGEKIGCFGLTEPDNGSDPGGMKTKAVKDGKHY
                     ILNGAKMWITNGTVSDVAVVWAKLDGVVRGFLVEKGTPGFTAPEMKGKFSLRASVTSE
                     LVLQDVRIPEENILPNGSGLKAPLGCLTQARYGIAWGALGAAMACFDSSVRYAKERIQ
                     FDKPIGSFQLVQEKLAYMITEITKGQLLVLQLGRLKDSNKMKFHQVSMAKRNNVYIAL
                     EIARIARDLHGANGIIDEYPIIRHMMNLESVKTYEGTHDIHTLIIGESVTGIPAYK"
     gene            14070..14510
                     /locus_tag="PK937_03620"
     CDS             14070..14510
                     /locus_tag="PK937_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31691.1"
                     /translation="MKICPNCTTNNLDTSAYCVSCGAELTIPVKEEPARESRFPKDHI
                     THREDHPASGMAKLAMTLGLISLFFVFFAVICAPLPGIMAIIIGNNELKKLRGERYST
                     NGETYCQIGIWSGWISTAIGILLIAIGGIATIWLVNWFIEAFTR"
     gene            14550..15203
                     /locus_tag="PK937_03625"
     CDS             14550..15203
                     /locus_tag="PK937_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31692.1"
                     /translation="MFIGHFATGFISKKWMPHISLPVLFIAVQWPDLIWPVLLLLGWE
                     SVRIEPGNTVMTPLAFDHYPYSHSLMTNLLVASLFALWALRRYRDQRVAALLFITACS
                     HWILDFVTHRPDLPLSPGSSQMFGLGWWNIPPVAVVAELALMAAGIFIYHRNTRATGK
                     TGVWAFWGLIVFLMIIHTGNLLGPPPPNITGIALAGNLMWLFVLWAYWIEKNRTVIK"
     gene            15238..16617
                     /locus_tag="PK937_03630"
     CDS             15238..16617
                     /locus_tag="PK937_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH31693.1"
                     /translation="MKQWFCVFLIIMVASCQKPALKQPDVKHLEPAAMTLMKQGLNDL
                     HAYTMLSELTTKAPKRLSGSTMADSAVAWGERTMKNLGFENVRLEPVMVPHWVRGPVE
                     EAFITSTAPSQKLNICALGGSIATPKEGIESEIIEVKSLQEVRTLGDKVKGKIVFYNR
                     PFDKTLFAPFEAYGGAVDQRGYGAVEAARFGAAAVLVRSMTNRIDTYPHTGAMRYNDS
                     IPKIPAAAISTQDANNLSAMLTKGQSVKVRMKLTCETLADKLSYNVVGEIRGTEKPEE
                     VIVIGGHLDSWDKGTGAHDDGAGVVHSIEALRLMKATGLLPKRTVRVVLFMNEENGVR
                     GGKGYAAVERPNEKHIAALESDGGGHAPRGFGIEADSVKLPRITRFESLLKIIDADRV
                     GPGHGGTDIEPLGKTGVPCIGLHPDPQRYFDYHHSDHDTIDKVHDRELELGAIAMAIL
                     TYAIAFEGI"
     gene            16678..17520
                     /locus_tag="PK937_03635"
     CDS             16678..17520
                     /locus_tag="PK937_03635"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HNH31694.1"
                     /translation="MKPVTEINTVHLFPELDRLLIACLRNLRPEDWRVQTSAGSWTVR
                     DVALHLLDGNIRLVSAMRDHHFAPAPNDLSSYEATVQFLNTLNADWLKAFERVSPAMI
                     CELLEITGAWYCAYLKTIRHNDDAVFPVAWAGESVSKNWFHVAREYTEKWHHQQQIRS
                     SLGDEETLLQNAWYLPYLDTSMRALPHVYRNMPAPDGIVLQFSVSDLTDANWFLKRNN
                     NKWELFSGTDSKPHAHMRIKKKAAWRIFTKNLNGERARPYIHMLDGDEFWLNPLLNMV
                     AVMA"
     gene            17572..18072
                     /locus_tag="PK937_03640"
     CDS             17572..18072
                     /locus_tag="PK937_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31695.1"
                     /translation="MIIAETDRLILRRLTHEDKHFILRLVNEPSWLRFIGDRNVHSPE
                     DAVRYITDGPMAMYAKYGFGLFLVALKGSDTPIGMCGLLKRDTLEHADIGFAFLPEYW
                     GQGYAYEAAAATMAYGQKQHGLNKIIAITSPDNASSIKLLKKLGLVEKEIIQLPGYSG
                     ETLVME"
     gene            complement(18128..18937)
                     /locus_tag="PK937_03645"
     CDS             complement(18128..18937)
                     /locus_tag="PK937_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31696.1"
                     /translation="MKSMRLLVLGVLTLTMLGCDSNNSNTLIAYVYQHEYLGSEGNDF
                     DYDGIVMGDPLPFIESVTAKNITGVQELYVSQNSFDFDFLYDATFGDGDITIKTTEGT
                     ITGTVDQPTMYDSVKASTTDYYIPLNTALTLTTYGGNSDFKYISAELEYYDGESWYYV
                     EMDSAFEGNTFTFPASMLDTESYLYIYGIDCANGPIAKAGSSGNMNGDGSGFMWHIIS
                     DDNFYMSFAVGIEAGSNVKKSSSHVQERRTREDRKRRFQEKTAKILNASAM"
     gene            19140..20534
                     /locus_tag="PK937_03650"
     CDS             19140..20534
                     /locus_tag="PK937_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH31697.1"
                     /translation="MKKYPVLSLRERVRPHTDYDGSGVTIAFVDSGYYPHPELRGRIL
                     KFVDVSSEAFTEKDFHTIRPSSWHGTMVACSACGNGIWSKGYYRGIASGANVVLIKAF
                     DGKKVKSSYILKSLTWIIAHHKPYHIRVVSVSVGGDRNEPSEKSRICKAIHRLHQLGI
                     TVIAAAGNNPSRPVIPPASCRFAIAVGGIYDHNDKNPDTASEYGSSYGITLDGLSKPE
                     IVAHARFLPAPMLPDNFTYEESQILHVLYKTPANNLKRKLRSLIHKTRLDAAILSLNP
                     AEIKKAIRQRMDGEKFIAPGFQHVDGTSFAAPIIAAVVAQMLEANPLLTPTMIRMILE
                     TTAKRVPGIAPEKQGFGLVQAKACVEQALIETSMPEIHESPLILKDRIVFLYYHRNIR
                     KVNLVGDFTDWQKNKLLLVEIRPGVWRVDIPLLSAGQYHYKFFVDDTLWTEDPLNPHK
                     ISDPFGGMNNILHI"
     gene            20734..21321
                     /locus_tag="PK937_03655"
     CDS             20734..21321
                     /locus_tag="PK937_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31698.1"
                     /translation="MKNLLKIFTFVLATNAMVTAQTIEVANEEPLTPEPKSTYYETKN
                     RIGFAAGNVSGVGLSYERDLFRNFSALFVIGGVAQKSQSDFNTGLTFKYTFSRINKRF
                     SVNVVSGGSYFLDIDDLGVYDNTNENRSLERRRSYIKMGLGLGMTALFFDGRLGIDVN
                     FIGMGASFQKKQNKKDYQLGDKPIRGPQVNLTYHF"
     gene            21557..21629
                     /locus_tag="PK937_03660"
     tRNA            21557..21629
                     /locus_tag="PK937_03660"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21590..21592,aa:Phe,seq:gaa)
     gene            21763..22788
                     /locus_tag="PK937_03665"
     CDS             21763..22788
                     /locus_tag="PK937_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNH31699.1"
                     /translation="MFLSKHPNGTYYLWYVDENNKKQKISTRSKTKADALKFLKDFNL
                     EKYRQSERIRCIHLSEFTCDYANYSKANHRPTTTRTAITALKELSRKMGNPLLSRISV
                     HDIESFFTSKAQECNSWALLKYYVTLKSIFNTAKRWEYITVNPFSKIPKPRPAEQTPV
                     YFTHADFTKLLRAIDDSDFKELVLFALMSGMRQSEIVHLTWSQIHFEKRLIQIRNTEC
                     FTTKSGKNRNVPIGEELLILLEMKYQNTMEKHSYIFQRNGKPYRHEYVSKKLKQYIRK
                     TELDDRLHFHSLRHSFITWALERGASLHQVSKVAGHSSTAITEKVYGHIKAEELHHVA
                     NLITINK"
     gene            23005..24717
                     /locus_tag="PK937_03670"
     CDS             23005..24717
                     /locus_tag="PK937_03670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNH31700.1"
                     /translation="MTIQSHREKANFIWQIADLLRGNYKQSEYGKIILPLTVLRRLDQ
                     ILEPTKAKVLQVYDDNKKQKPEILEKLLNKAAKQKFHNRSKFDFKKLAADPNGLEKNL
                     RNYINGFSAGAKEIFDYFQFEDEIEKLDKENLLYLVIKDFAKQDLSDLDTMQMGYVFE
                     ELIRKFSELSNETAGEHFTPREVIRLMVDLLFIGDNDILRKEGIIRTLYDPACGTGGM
                     LSVAEAYVQELNPNARLEVFGQEINDESYAICKGDMLIKGQNPGNIKYGDTFTKDGLE
                     NELFDYMLCNPPFGVEWKKQKEVIEKEHDDRGFAGRFGPGLPRINDGSLLFLLHMISK
                     MKKDGSRIGIVFNGSPLFTGSAGSGESEIRKWIIENDMLEAIVAMPDQLFYNTGIATY
                     VWILTNRKAKKRKGKVQLINAVSFFEKMTRSLGNKRNLISETQLTSIAKIYGSFKSGE
                     FCKIFDNEDFGYARITVERPAKDEKGQVLTDKNGNPKADSELRDYENVPLTEKIETYF
                     KREVLPHVPDAWVDESKTKIGYEINFTKYFYQYKPLRSLSEIRADILALKKETEGMIR
                     EVIK"
     gene            24829..25479
                     /locus_tag="PK937_03675"
     CDS             24829..25479
                     /locus_tag="PK937_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNH31701.1"
                     /translation="MDKAWFQKVFSEERTQKYFETYPANDKKAIEHYACNIMISEAFY
                     PALSIFEVALRNSVNRELETMFHTQDWYQQIPTTAGLVKLNKDISNAKRQIMMRNEAI
                     TASKVVAELTLGFWVRLFNTEYERILWKNLRLAFPYLEKRKRQRHTVSAPLNRIRDFR
                     NRVFHNEPVCWNINSLENNHKTIVETLFWLNKDLPDYLNTTERFHTVLNEVRKILS"
     gene            25476..26810
                     /locus_tag="PK937_03680"
     CDS             25476..26810
                     /locus_tag="PK937_03680"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNH31702.1"
                     /translation="MKAYPKYKPSGVEWIGEIPEKWELTRMKYVAPLQGGYAFKTEAF
                     KTEGIAIVKMNNLKRGRLDLSDAVRVDFKAAVDAFSLKAGDLLFGMSGSLGETGSLGN
                     FAVVNLVDIPCQLNQRVGRFVALQNCSLDFVRFLITSVSFTEPIMSSSTGTAQFNISS
                     EQIGNILISLPPLPEQTAIADFLDRKTAQIDGFIAKKQKLIELLREERVAVINHAVTK
                     GIDPKAKMKPSGIDWLGEIPAHWEVKRLKFFANVNPTKCNYVFDKSSNELVVFLPMEN
                     VLEDGQIIQDLRKPINELSTGYTYFEKNDVIVAKITPCFENGKGALLDRLETPFGFGS
                     TEFHTIRPVKAVSTNEFLYFLTKTDLFMKFGEAFMVGAAGQKRVPPNFVENFMIASPS
                     LDEQNHIVAFLKSETAKIDRTIATIEKEIELIQEYRTALISEAVTGKIDVRG"
     gene            26968..28113
                     /locus_tag="PK937_03685"
     CDS             26968..28113
                     /locus_tag="PK937_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNH31703.1"
                     /translation="MDIKDFKSGIFRQQYEYKSFLPNSIHREWVVSDARLNNLLSEAN
                     LTLGRLDAFSRLIPDVDFFIRMHIVKEATQSSRIEGTQTTMEEAFLKEADVNPEKKND
                     WAEVNNYIFALKYAIGELKKLPLSTRLLRQTHEVLMQGVRGRHKMPGEFRSSQNWIGG
                     TSLKDAIFIPPIHTEVPELMSDLEKFLNETRLQVPHLIKIGLAHYQFETIHPFLDGNG
                     RLGRLLITLYLVRFGILNGPTLYLSDFFERNRSLYYDNLMQTRLQHNLPHWLTFFLTG
                     VLQTATNSIETFEKILKLQEDIEGKRILKLGKKSEAAKTLVKHLYKNPLVTASDVEKI
                     LHVNISTANRMIDDFVKLKILKERTGFKRNRIFSFHEYLGLFYNPRK"
     gene            28110..31079
                     /locus_tag="PK937_03690"
     CDS             28110..31079
                     /locus_tag="PK937_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNH31704.1"
                     /translation="MINTEKIFESAIEDHLLESGWLKGRAEDFSRDHALDAGQVIGFL
                     KDSQPKDWEKIAKIHQSDVDTKVVNRLSKELELRGTLDVLRYGFTDSGVRFRMAYFRP
                     ETGLNPDTLALYAKNRLIVTRQVKYSPKNENSIDLLLSINGLPVATAELKNPFTGQTF
                     KNAIRQYQHDRDPRELIFQFKKRALVHFAVDPDEVHLTTKLDGSHSRFLPFNKGHNNG
                     AGNPPNPAGYKTAYLWEEVWQKDSWMDIIYRFMHLQVEEVKVGRKREKKESLIFPRFH
                     QLESVRDLTNDARKNGPGKNYLIQHSAGSGKSNSIAWLAYHLSSLHDATDKRIFDSVI
                     VVSDRTVLDQQLQDTIYQFDHKSGVVQKIDENSNQLAESIKAGTPIVITTLQKFPFIL
                     DKAKALPGRNYAVIVDEAHSSQGGESSKKMKEVLSAQSLEDAEKEEGAETDDGEDEIR
                     KSMAARGRQSNLSFFAFTATPKAKTLEVFGTKGADGKPRPFHLYSMRQAIEEEFILDV
                     LKNYVTYKTFFKLSKAIEDDPELNKKKAAIAIGRFVSLHPYNLAQKTEIMVEHFRQVT
                     SKKIGGKAKAMVVTGSRLHAIRYYFEFQRYIKEKSYSEIKALVAFSGKVIDTGYPEGV
                     SESELNGFGEKELPDKFETDEYQVLLVADKYQTGFDQPLLHTMYVDKKLSGVKAVQTL
                     SRLNRTCAGKEDTFILDFANDVETILESFQPYYELTTMEQTTDPNLLYDLRHRLDEAQ
                     VYLQSEIDALAKVFYNPNGSTKDQARLNALVDPAVDRYEDIDADEKKDEFKKSLTAFV
                     RLYAFLSQVMPFSDIELEKFYVYARLLVSKIQSPDYGGRLKLDNDVALQYYRLQKTSE
                     HLMVLEKLPEYSLDPTTEAGIMREKEMKAKLSEIIEILNTRFGMDLDEADKLFFDQIE
                     VELLQDETLRQQAVTNSIDNFRYGFQEKLDSTMLDRMESNQEIFQKVMENDEIKTAVL
                     EAMIKQVYAKFNNKNLQ"
     gene            31274..32188
                     /locus_tag="PK937_03695"
     CDS             31274..32188
                     /locus_tag="PK937_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31705.1"
                     /translation="MGKKQKKSKPRADSGIQMRSINIEDLNHKYGYDSKSTKPVKGFD
                     SIEYSMYRTRLIGHVDGGIFNEVNGLYSQKRFWAYLTKVLKPNAQRIAFFKDLMKGIP
                     NGTALKSQFDSAFKNFWKSLRQEEIEIELQGDTISLPSYLNLLLFDEFKKTYYHRVFS
                     LIVENTVFFDLKIKPSKEAGKVLSELAKSLANEIKRGLSLELEGFFGYQIRLAKIAVG
                     DPQQIKREFEKLMRYNQKVKVYCEEKHFPKGVLLPIEVRYNLVKHLKKVTYPSSEFSS
                     KGQGAKDMILINKILNELGYTTKRRSHS"
     gene            32394..32789
                     /locus_tag="PK937_03700"
     CDS             32394..32789
                     /locus_tag="PK937_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31706.1"
                     /translation="MHNKPNPSTVKSQIHIHPSGHVYLATETRKRFIGKIITREFHCE
                     RDPERHILRVNNSIGFNLELMRMSEQFDWVIITGLSSQPIIVTRDFILRKGFLPSFKN
                     QSYELQIFVKLNDLKKADTEPDLFSPEAA"
     gene            33068..34612
                     /locus_tag="PK937_03705"
     CDS             33068..34612
                     /locus_tag="PK937_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH31707.1"
                     /translation="MYREISKSGYGNYHLWIFFDEDISAKFIRSVLSAFIIQVLKPET
                     ALEIFPKQNAGDKGNFIWLPFFGKDVSENKTVFIDASGKKYDAEFKPNSRKTLDELAD
                     FYHLELKETTSAKVSRKTDYSEILSNGVPVGERNSTVTRIAGHLKHAGLPISETKAIL
                     QLWNKNLASPLSDKELCTTVRSIYSYSTESAQDTIYHKGGAMLKMPIPVHYDLVEGLM
                     HEQSVNFLSGEEGCGKSLLAMNLAISVASDKPNFLGYNITKHGKVLFLNNELGFPDFL
                     IRFQKMSVGLNPEQLDNFITPTTVPPLNEYYSELDKLLAAEKPCLLVLDCLYWSHDLK
                     ENDSSEMKSLMRQLISLRDKHRLCILVVHHTKKGSRYQKMHNDNMRGAGVFGSATDSV
                     FQFRRSAIDDTKRLFKPTKLRHGADGLRHAHLIALNDETLWFCDEGKTDEDEHIATLT
                     TPKKDIFEIDFSAIMKPGEKVSRKEILEKCKSLGCDERTIDRKLKEAKDRSIIASVKN
                     GQYTLT"
     gene            35068..35139
                     /locus_tag="PK937_03710"
     tRNA            35068..35139
                     /locus_tag="PK937_03710"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35100..35102,aa:Phe,seq:gaa)
     gene            35416..38613
                     /locus_tag="PK937_03715"
     CDS             35416..38613
                     /locus_tag="PK937_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31708.1"
                     /translation="MSITSEKIFRLLFCVFLFLFMLAADTKALDPERSVTQYVHDVWL
                     KEDGLPGISVLSFYQTREGYLWIGSFEGLARFDGAKFTLFNRRSIDAFLSYGLWRILE
                     DKHGYIWMGTNGNGIYRFKDPNFQRLAVTNGLTNDNVLSLAMDQDEMVWAGTRKGLNR
                     IRIISQDSFAVEPVMYRSNVLQDVFGLCTDHDGTVWIGATNTIYRYVEGRLEEFANIG
                     ERIRSMYVDRDNRIWIGTTNAGVFQISENQLRHFTTKNGLPPESIWRVWYDRDGNVWL
                     GSDGMGLFRAKPGSFRDENELISLEQFSEKEGLTNNRISALFEDREGSLWIGTFRGGL
                     NRLRDGKFITYASANGMVHPVVYPIVQEHNGDMWFGTEGGLSHYSGNRFVNYTTANGL
                     SNNQVRGIVRDSRGRLWVGTYGAGLDIFNPASGRFAPAPSAINHAFRNIRDMLKDREG
                     HLWIGTINSIGLIKPDGSHRTFTMQDGLLTTSILCLYEDIYGNVWAGTDGGGAARYDR
                     NKGFFRVLTMKDGLSSDIVFAIHCDRDSVYWFATQSGITRYDPRATDLPFASLTVRQG
                     LPADAAFEILEDNHGYIWLGGAEGIFRISKKDFEDVARGKTNQLTPLLFGRPDGMKTS
                     QTTPSGRAHKTSDGKLWFPTPKGAVVIDPDKIKTNPVPPPVVIERCIADGQSWPIQND
                     VVLPPGILRIEFQYAALSFLAPQRVFHKFKLEGFDTDWMDAGTRRTAFYTGLHPGEYT
                     FKVIACNNDGIWASEPTSFSFTIRPHFYETRWFLLLIFGGMIAAALSLYRWRVRHLMH
                     REKELAALVKLRTHNLEEEKKRTEDARIEAERQREEALRANELKTQFLGMASHDLKNP
                     LAAIISYTDLIIEAYDDKAQSMRDLGYIRKAAHRMLHLINGLLNTVSLESGKVVLQKE
                     KLDVSDLAEQVLFSNQQMLERKRQHVQVQTVPGLYVNADRDRLYEALDNLISNAIKYS
                     DYDKTIRVDLGMNELKIIIRITDEGPGMTTDDMKKLFGKFQRLSAKPTGGETSTGLGL
                     SIVKQIVEMHEGQVWAESESGKGSTFVVELPRFTS"
     gene            38908..>40698
                     /locus_tag="PK937_03720"
     CDS             38908..>40698
                     /locus_tag="PK937_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31709.1"
                     /translation="MTQYMHTMWTTDQGLPQNNITSMLQTRDGYIWCGTLDGLVRFDG
                     VRFEVFDKRNTPAIRNNAIITMFEDRSGGLWVGTIGGGLVHYKNGKFISYGLTEKFPS
                     AYILHMAEDEQGRLWFATRDGLVTRGDTSWITYTAKEGLTHTSVRSLFFDRKGRLWIG
                     SSVGLSLMENGVIRKIFAHELADKNVTSITSDSTDIIWITVLGKGVYRIKGDSIAHLQ
                     APKSIPHNFINAIARDKNGNRWLATDGGLYREHHGRFERFMDESNFPLDKVMCLLEDR
                     DGNLWFGTNGSGLNRLRDEKFITYTTLEGLKDNSANVVFEDSRARIWVGTEAGVSIID
                     GDTIRNLGESSGLTNTKVRSFAEDTDGRIWVGTREGVFVWNEVRFESWNTRLKIPPLV
                     ILSMLRARDGTMWIGTGGAGAVSYKPQNVQQYTSKQGLAGIVVIAMAEDSLGRMWFGT
                     NSGLSRFDGKNFVTYNSKNGMSSDIFLSLYVDKTNTLWAGTFGNGLNRLQNDSIAIFT
                     TADGLFNENMYSILEDGEGYLWMSCGKGVFRVAKEDFERYRQKIISVIPCKVFGRSDG
                     MRSSECNGTTQPAAWMTRSGWLLYPTGKGLA"
BASE COUNT        11537 a         9387 c         9011 g        10763 t
ORIGIN      
        1 catcaatcag tttcaataac cgctcttcac cgtattcgtg acgatgaata ttgtgtgctt
       61 cgatcacgcc atccgttaca aaaacaaaga tatcccccgg tgcatatgcg aattctttct
      121 cctctagatt ctcctcaaat gttccgtcat cggttatgcc caaacctacg ccaagcggcg
      181 tgataagctc taccttccgt tgggacgcat tgtaacaata caacggtaag tgccctgcac
      241 gtgcgaggcg aatttttttc tcttccaata gaaacgttgc accgatcgag gtgacaaatg
      301 attttttctc caaatcacgg cacaaaatac gattggaacg aataaacaat tcgcgcggtg
      361 tcagatcaaa gctgttgagc gaatgaataa taccctgcac tttggacata tataacgccg
      421 ccgacgtgcc tttgccgctc acatcgccta cgataacagt aaatttatcc ggtttaccgt
      481 tgagataatc gaaataatca ccgcccactt cgagcgccgg taccgaacgt cctgcgattt
      541 ccaagcctct cacgttcggt gttttttgag gcaatgactc caactgtatg cgccgagcga
      601 tattcagctc atgcttcaaa cgatcttgct cggcaagatt ctcgtacaga aaggcattct
      661 cgatcgcaac cgaagcttgt ttggcgaccg tcgttaaaaa cataagatca tcatgatcaa
      721 acggcgattc cgacatgcgt tccccgacga tgatcgcgcc gacggtgtgt tcttttgagc
      781 gaatcggtat aacaaaacga aaaccgcatt ggcgcaattc gttgcgcagc ggtcccggta
      841 aattatccac attgatcgta gctgttgacg tattggcgcg cacagccgcc ataagaggtt
      901 gatcggactt cgagcataac gcctgccacg cgccggattc atacccatat acttcactgc
      961 aacacaacga tttctcatcc cgataaaaaa cgacgccggc cattttgaga tgcataagcg
     1021 aagctaattt ttcgaccatt ccgcgcgcca aatcattcat attaagattg gtcgccatca
     1081 cctccgcaag ctctgccgcc gcacgccggt agtcgtgatg ggatcggtga aatttataat
     1141 cgatccagcg ttggccccat gatcgcagac gtacccaaac aaacgtcatc gccacggcca
     1201 acaatataag aacgacttta agaatttgta tgcgttcatc cgcacttaca ggtacatccg
     1261 taatttcgac agaagattcc gtaaaacgga tgttaggcaa atccatattc gaagtcaccg
     1321 cgatgaccat caattgcaca gcgccataca ccacggtcaa attccacacc agcgatgcga
     1381 tcacatattg tacattgcga cgcacacgga agtttagctc catcaagtgg tatcgtgcga
     1441 tcgtgtacag atgcgcgagc gggatcagcg caaagaagaa tccggcataa ccaaaaaggt
     1501 agccacgtcc tgaattaccg tcactgaaaa acgtcaatac gtaacccaaa acagcgcatg
     1561 cgaaagcaat ccaaccgctc agacgtgaaa ccgaacgata tgatgatgaa aaatatgact
     1621 tgtaacgcaa tcggataaat atatagtaca ccatcatcgc aatgccgctt acaaacgctg
     1681 caagcgcttg tttgtaaact acaattccga taaacgttat cgccgatagg ccatagggta
     1741 cgatggctac ccattttttt tgaattaatt cctgtacgga aaacggaaaa tataatcggc
     1801 tgtgcatcag catggccata ccgagtacga gactccacca tcgcacattg gtcacaaata
     1861 ccccgaatgg cgtggtactc aaggatccgc gctcacctaa aagcgacaac atcattccga
     1921 taagaaaaaa cgccattccc aaaagcacgg ccgctgtggt atccgcgcgt ttgtatacca
     1981 aaaatgatcc tactaatata tacacaagac cggcgccata aatcaccaac agaaaaaacg
     2041 gtatgccaaa cttggccagc gtaacatcca aggtaatgat ttggttgtcg cgcaaaatat
     2101 cgtaagcaat ggtcttaccg atttgtccgg atgacaaaat tcgatcggct tcgatcgccg
     2161 atttgaatgt tcgcccgttg atacgaacaa tcaaatcacc gaccaacatg cccgcgcgat
     2221 cggaagcgcc gttgtgggcc acttcacgca catgcgccgt aaactggatt tcatggtaaa
     2281 aactgtccgg ggcatctttc cgaataaaat tggctacgaa cattttggat gctttggagc
     2341 gaaaaacaat ggcgttgatt ttttcttcag atccgacgcg tttcaaaaat cgggctatgg
     2401 aatcctgatc tttccacaag ataccgccga cggatacgag caagtcgcct gcatatacgg
     2461 aatcgcgaat aaatacacct gtcgtatcgc gtttttgata ataactgata tcggccggaa
     2521 aactgcgaaa gatgtatagc tgagacggcg catcatcaaa aatattttcg tctgtcggag
     2581 aagcggcaaa acgataaaaa ttgacaacga ctaagactac ggcagcgatc ataaatgccg
     2641 tcagaaaaat gcgccgaaat aggaggtgtg tgggcttcat ggtcataact tatggcttag
     2701 gacgcaatgc tgcaagagtc aaaaaacact cttacaaaga ttacgcgcta tgataacaaa
     2761 agattcacta tttatatgcg catccggttc atgcccgccg gaatacgttc cgattaccgc
     2821 cgcgcagagc ataccgatac attacaattc attctatcga atcacatgca ttcccgatag
     2881 tgctaaaatc aatatgccga gaacgatgcg ataaacgata aaaatcgtcg tcgaacgtgt
     2941 ctgtagccac ttgattagaa aagcaatgga agcatatccg acgataccgg cgacaagcgt
     3001 ggccacgaaa agatgaatca actcttcgcc accgaggccg tatttaataa ggtcttttag
     3061 ttcgaaaagc ccgctggcta gcacggcagg gatcgaaagt agaaacgaaa atcgtgcggc
     3121 gctttcacgc gtcaaaccga gaaataatcc ggccgtgatc gtcacccccg aacgcgatgc
     3181 gcccggtatc aatgcaactg cttgcgcaag cccgatgatc tgggtgtctt taaaatccat
     3241 gtcggcaata cgacgacgat gtttggccac cacctccgcc aatgctaaga tcacagccag
     3301 tccgatcagg ctccatccta tgatggtgag gctacgaaaa tcggtttcaa tcgtatcttt
     3361 gaaaataatt cccagtattc cgagcggcaa ggttccaagt gcaataaacc aagccaatcg
     3421 tacgtcctgt cgtaactcgg catcccattg cgctttctga ctccatcgtg tcacggaaaa
     3481 aaaagccgct ttgataagac ggataatatc gtttctaaaa aagatcagca ccgccatcag
     3541 cgtcccaagc tgtgtcaccg ccgtcacggc ggcgccggga tctggccaac ccaaaagcgc
     3601 cggcacgatg cgaacatgcg ccgtactgct gatcggcaaa aattcagtaa tcccttgtat
     3661 gataccgacc actatggctt gaaaaaaatc catacgtttc ctgaagttta aagtaacaat
     3721 aaaaaagccc tacaccaatg atgtagggct tttttccgca tatcagaaaa attataactg
     3781 tgtttgaacg ttggcaatca ctttattgat gatcgctttg agcgtgtcaa aaacacctac
     3841 gcctttgata ccgacggctt cataatacgg tacattaaaa taattgaggc gtttttgaag
     3901 attttccacc gtatcaacat ccggaagatc gcgcttattg tattggatca cccaaggaaa
     3961 cgaatccagc gagtgaccgt cttcacgcag attgttttcc agatcaagca atgtttcaat
     4021 attgccgtcc atacgatgct tttgcgaatc ggcaacgaaa acgataccgt ccacgccgtt
     4081 gaggataact ttacggctgg aagcgtaata aacctgaccg ggaacggtgt ataaattaaa
     4141 tttcggtttt ttacctttga tacgaccgac ttcaagttgt aaaaagtcga aaaatatcgt
     4201 acgatcttct ttggttttaa gcgatacgag ttcgccggcc agcgagggat ccattttgga
     4261 atgaatgtat tccaaattgg tcgtcttgcc gctcataccg gggccgtagt acactatctt
     4321 cagattgatc tcttgtaatg cccagttgat aaacattcgt atcctctcta aggttttatg
     4381 gttgaatcag cagaccggat aatccgttat ttaaacgatt cgtcgagtgc atccccgagg
     4441 agcgaaccga attcggagtc aatcaggtct tttgcggaat catcgctgga attggaagaa
     4501 tcttcaaaaa tttcaatcag attctggatg gcttttttcg tgtaaatacg aatgaggccg
     4561 agcgttacac tggtatcaaa cacgacgacg aggaaaaaat tgtcgccgac attggaaaga
     4621 taaacattgc gtttttcacc ttcgtggaaa agcaatttga atttagcgtc ttcgccgaca
     4681 agtttggcca tttctgccgt cgctgcaaaa tccgatgcag ctaacgccga aagcactgtc
     4741 gtgttcattt tgtccgtatt gccgcgctgg ctgatgagct ggccgctcat atccgcaaaa
     4801 agaatcgccg aagctttcgt tttcgacgcc agctcagaca aaactttggt aacgccctca
     4861 aattgtttcc cgttaagaat aatttgcttg ctaccgtctt tcaatttttt cgtctgcaac
     4921 atagcggtta ccctttgctc tggttatggt aatcttaaaa gaataatcaa aattggatct
     4981 tgataagcgg acgtgcatcc gtattacgga ttaacaaccg taacctatgg aattttcgtc
     5041 ctttagtcaa ataatttttt aaccggcctt tttctttcgc aaacgttcga tagccgcttt
     5101 caacgattca cgcaaacgct caatcgcatc cgccaaatca ccgatttcat cggtcgcgtc
     5161 gacgcgcacg atggattcta aatcgccttt actgatctgt tcggccgact ccgtcaggta
     5221 caataactgt ttggtaagtg cgttcgaaag atacaacgcg acgatcatac caaaaaccac
     5281 ggccagagca agtacgccgc ccactaaaag cgccgtagca cggatcgtgt catcaataaa
     5341 ttttccggac atccctacgt gcgcattgcc caacgtaccg gcgccgatcg gtgtagatat
     5401 ttcgtaggtt tcggtatttt cgacttgagt caagtcaatc ttataatcac cgccgccgtc
     5461 aatcacggcc gcttctacca tcgtgcgttg attgtacgaa ccgactttaa atttctgtgc
     5521 aaccacttta ttttctttgt cggaaatata cacgtaggat acggccgtaa aaccggaagc
     5581 tatatccacg agattctgga tgtccacttc atcatcgccg accagcggac gttccacctg
     5641 tttggacagt gtggtcacga tcgctttgcc gcgctctgtc agttcggact gcagcgaacc
     5701 cgacaccaac tgtaccacaa gaatggtaat cgtgataccg gcaatcaacg caccgctcac
     5761 gatcacgagc ggcactttcc attttaggct tagtcctttg gccataatcg tccgatcaca
     5821 atgtacggat ttgtttgagc atcagttgtt taaaggccaa ttgtttggcc ggatcgtata
     5881 tctcattggc tatcgcctcc accagcggcg gcactttgtc tttgggaaac gttttctgat
     5941 cttcaccaaa cgtctcaacc acgtcgtcaa taatgatcga agccaccggc ccgatcaatt
     6001 gaatcaattc attttccaga tttttaaaaa atgcagaaga tacattttgt ttatcggccg
     6061 acgccgtagc gccggaaacc gccgtgatca atccggcttc ggccagcatg tatacgatcc
     6121 ccgccacatc catatctttc ataccaagac gcgaggataa ctcatccaca gagtattcgt
     6181 ctttttctaa gagatgtaac aattcccagt ctttagcttt aagacgaatg gtcggcgaag
     6241 gctcattatc cgttttacga aatttgacgc ggaacgaagg tatgtggcgt gcaatttttt
     6301 gccaggcttc atactctaac gtcgcttctg tgatgagctt gcctgtttct ttatcaatgc
     6361 ttttctgtag cgaggccaca tccggttcaa atgaaaactt accgctggtc cagagtatca
     6421 actcgtggaa ggcgggttcg ccgagatagg tatcgcacac ggcatggacg atacgcttat
     6481 tggccagata aatttcccca ttttcgcgtt gatcgccgcg ctccagcaaa atgcgtcctg
     6541 tacggtcacc tgcgaccaat aacttgacga tgctcagcac gtcaacgtga gataaatttc
     6601 cggagagaac ggttgccatg gatcaggccc gcacgtgttg gttaattgac aacttcgacc
     6661 agtgcccagc tttccgcatc catattgctc cgcaccagaa aactgcggcg ggacgcttca
     6721 actttactga gtcttttttg aaatttttta tcggcttcca gatccgccag cgccacattg
     6781 actgtgaggc gcaacatcgc cacgctcaca ttgggctgac acagaatgat caggttaaaa
     6841 ttactcatcg gataggcgat gacgcgccca ttatcgtatt tgagatcgta ttcatgcagc
     6901 ggcgtggaga ggcggtcaaa catggcggcg acttgaatta catcgcgcgc gatttccaca
     6961 tatgtcgctt cgtcgaaacc ttgcggcata ttagcataca ccgtttcgcc gtcattaaaa
     7021 taaataaatg aggctttgac accgttgacg ctattgattt cttcgagttt gtctttcata
     7081 atcctgtccg tggaaaacgc ttattcggaa actttttgaa taccgggcgc tttttctatt
     7141 gcctgctcga tcagatccgc tgccgctttt tgctctacgg tcagcgtgat aatatcgtca
     7201 tgccacttgg aaataatttt tctgtcattt ttgcccgtta cgatgccaaa cttatacgtg
     7261 cctgatttgg aagcacggcc gatcttagcc agtttgcgca tgataaaacc cataccggca
     7321 gaggtgcgtt ttccgttggc aataccgtct tccatcagga tctcgccctg cggtgacgat
     7381 accagcacgg cgtttacgcc gttgatggtt ttgatgtcac gggtaaactt ggccatggct
     7441 tcgcctttgg cctctaaggc ttcatctatc agacgaaggc cttccatcat cagattgccc
     7501 cacggcgcat tgatcgtacg cttcggcgaa gcaacgccgt tttcgacttt aaaagggcct
     7561 tccttgatgg ccaatatttt atatacggca tcttcacctt ctacagtacc aaatgtcgcg
     7621 tgtacaatat taccttccgc aaaataaata cgtccgatcg cattgcgtaa ttcgaccgta
     7681 aacatcgctt cgttcttttc catacagttc agttgaacga tgttggtcaa tttgagatct
     7741 cttagatttc ctacaagcgc cacgtcaata ccctttattc ttattttaac gcttcggcaa
     7801 ttacgctttt catcatcttg atttcaaacg gtttttcgat atagcggtat gcgccaaaac
     7861 tcagcgcttc ctcttttttt tcatctgaac cgtatgccgt catgatgatc acatgcgtcg
     7921 gcgggcgttc tttttttacg cgtttgagca attcgatccc gctgatgccg ggcatggtaa
     7981 tatccgtcaa tatcagatca aacggatgtt cggcaaattt ttcccaagcc tcggtgccgg
     8041 aggaagcggt cacgacttcg taattttctt tggatatgat aaaactctgg tagagactaa
     8101 acgtaagcgt ctcttcgtca tctacaatca aaattcgctt taaagtaccc gacataaggc
     8161 ctcagattgc cattgaaata acatttgttt taataattac aaaaccgaac agcatcggtt
     8221 taacagaaaa cacaagctta ataatttgaa attttatgtt tgcgaccgat gattggaatc
     8281 tttactgcaa tgtattgcgt aatgattatt cgggtattct ttcagacttt caaattacaa
     8341 gataaattaa atagcgggga aatatatgaa aagcgaatgg agattgtcaa ttgaaaagat
     8401 atgcttcgtg aagaattttc aagactttag catcgtttta agacaaagaa actcctttac
     8461 tttttatttt tagctgtgta ttttctcaaa attttgatat acctgactta tattggttgt
     8521 atatagtttc gggtatcgtt gagggtgtca tccaataacc ggagcagggc atgagtacgt
     8581 ggttagtcga aaaatgtaaa ccgttttcca actttgtcga agatattaag gccaaaggct
     8641 tatacagtta ctttacggaa ctggaacgca ccggcacata tatcaccgtc aaaggtcaaa
     8701 atctcgtcaa tttttcatcc aacgattatt tgggtcttac gcatgatccg cgcgtaatcg
     8761 aagaagcacg taaggcgctg gatatttacg gatcgggact ttgcggatcc cgctatgtcg
     8821 tcggcacgac catcgaacat acgcgcttgg aagaaaaaat tgccgattgg aagcaaaaag
     8881 aaaactgtat tgtatttagt accggttatc agactttgct tggcaccgta tcggccatgc
     8941 tggtcaaggc cgatgattcg atcgaagatg atattgtgtt ggccattgac aaactgagcc
     9001 acgcatgtat ccttgacgga tgcgctctgg ccgtaggtac tcacatgacg cgcggcggaa
     9061 gtaccaaaaa tctggacgga cagatacggt attttcgaca caatgatccg ggaagcttag
     9121 agtccatcgt tgagtccagt gtcaaacgcg gtaaaaatgt gatgatcatt gtagaaggtg
     9181 tttacagtat ggacggcgac atttgtcgtc ttccggattt tgtcaatatt gccaacaaat
     9241 acgatgcgtg tatcgccgta gatgacgccc atggcaccgg tgttttgggt aaaaccggac
     9301 gcggcacggg tgaacacttc ggcatggaca aagatattga tctgctgatg ggtaccttca
     9361 gcaaagccct aggcggcgtc ggaggctttg tgtgtggcca gcgtgatgtg atctcccaca
     9421 tcaaacataa agcccgcagc ctcatcttct ccgccgcatc ccccgtttcc aatatcgttt
     9481 ccgtatccaa aatcatagac atcattcgtt ctgaacccga gcatcttcaa aatctgcgcg
     9541 tcaacgagtc gtacttcaaa tccaaactca aagaattcgg atttaactac ggcgacagcg
     9601 aaacaccgat cgtaccgatc atcatcggtg actcggaaaa aacgatgtac atttccaaaa
     9661 aattgtacga gaacaatatt ctcatgctgc cgatgatttt ccctgccgtt gcgcgcggcc
     9721 aggaacgtct tcgctgtacg atcagcgcca aacatacgcg ccaggattta gatcacgcct
     9781 tggacgtgct ctacacggaa ggtaaaaaaa tcggcgttat ctgatataaa gcaacctcca
     9841 tttttttaaa ccctgcaagc tttggcatgc agggtttttt tgtgttcggc cattttcata
     9901 cgccaattcc cttgattccc cctatttgct tctctatatt caaaacaatc gttttttacg
     9961 gcggtacgcg gcaccatgac aaaaaaatcg gtcggtaatt ttatcctcgt cctgcatacg
    10021 catctaccct atgtgctgca tcatggtcaa tggccgcatg gcatggagtg gctttatgaa
    10081 gccgcgtccg aaacttatat tccgctgctc aatgccctga atgcattgca aaacgaaggc
    10141 cgccggccgc gccttaccat cagcatgact ccggtcatta ccgaacaact tgcgagcgaa
    10201 acgtttaaaa aagaatttgt cgcttttttg tatgatgaga ttgatcgcgc ccgcaccgat
    10261 atggtgtctt ttgaaaaaag cccgacggag aaaaagttgg ttccgctggc ggaatattgg
    10321 ttggattttt atcaaaatac gctcaaagat tttacactca cataccgcca tgatttaatc
    10381 ggcgcgtttc gcaccttaca agatcaaggc gtacttgaca tcatcacctg cggtgctacc
    10441 cacggatatt ttcctctcct cggtaccgat gccaatatcc acgcgcaaat ccgaatggcc
    10501 gtcgtgacgc atgaaaaata ttttggccgt aaaccgcgcg gaatatggct tccggaatgc
    10561 gcctatcgcc cccggtatgt atggacaccg cccgtggctt cggcgcttgg ctccgagccg
    10621 cgtcttcgta aaggtatcga ggaaatacta gacgccaaca gtttggaata ttttatcgtt
    10681 gattcctcgc tcgtcgagcc gggtcgggcc gtaccgatgt acatgggacg gtttgaaaat
    10741 ctacgcaaac tgatgatgcg tttggccagt gaaaatcgcc cgatacctaa agcggatttt
    10801 ctgcaatcgg tatatgatgt ctataaaacc aaatcatcgt acgacgcacc cggtaatccc
    10861 gtttccattt ttgcaaggga tatggaaaca tccatgcagg tttggtccag tgaacaaggt
    10921 tatcccggcg gcgaatggta tttggatttt cataaaaagc acgagcaaag cggacaccgc
    10981 tactggcgcg tcactagtct tgaggccgac ctcggtctaa aagcaccgta cgaacctcaa
    11041 aaaatccaat cggccatcgc cgatgatgca cagcattttg tgcaagccgt gcgtcaggca
    11101 ctcactcgat ttaaaacaaa acacggatac gaaggcacgc ttaccgctcc tttcgattcc
    11161 gaacttttcg ggcattggtg gtttgaaggt cccggttttc tcaaaaccat cttcgaactg
    11221 atggatgacg atcccgcaat ccaaactatg cattgcgccg aatcgttgga tcagaatccg
    11281 cctgaaaaaa tcatcgctat tccggaaggc agttggggcg cgggtggcaa ccactatgtg
    11341 tggtttaatc atgaggtgga ttggatgtgg ccttttatct acaacgacga agcggctatg
    11401 cgcgatcatg tgcagcgatg gaacaataat accgatcccg aatggaccgc gatcatcaaa
    11461 caaatggcgc gtgaactttt tcttctcgaa tcttccgatt ggcctttttt gatcagcacg
    11521 cagtcggcga ttgattatgc gaccgagcgc tttatggaac accataccgc ttttgaaaaa
    11581 ttagcacgtc tgtccgaata tcattgtaac ggttccgcat tgcatccgga cgatcgcgcc
    11641 tggttacgcg aaatcacatc gcaagatcac atttttgatg atgcgctgat tgatccgaat
    11701 tggtttttga acgattgatt ttttccgctt atttttccta ctcaacaaac accggaggtg
    11761 ttatgaaaaa ttcccggatt ttagttttat tttgtttgtc gctcatggcc acagccatcc
    11821 atgcacaatc ggaccctttc acaggttcat ataccggcaa aggatttcgc cctggcgaaa
    11881 aatatacgga aaaaccgagt tatgtgattg atgtgacgat cgaaaaaacc ggcgattatt
    11941 atatggtacg ttggtttgaa aacggtaatc tctcgtatta cggactcggc atccacattg
    12001 ataatattct ctctgtcagt tatgtatccg tggataaatc catttacgga acggtgtcgt
    12061 ataaagattt taaaaaagac aaagggtatc tcgtcggcgc atggtgtatc gcgcccatgg
    12121 atcccgaagc cgtgggtaac ggaaaaaacg gaatggaagt cctctggccc aaataaagtc
    12181 ctcgtgaatg gcttgaaatt cagatttttt gagtttatct tgccgcgttc ggatgaaata
    12241 cacggaatat gaattgtcct ataaatttaa aggagttttt tatgaaaaaa tggatgatga
    12301 ttgcagcact gatcactgca tttacatttt ctcaaaatgc caacgctcaa ttgcctgtta
    12361 aaatgggacc gcgcgcaggc gtcaatatcg gtgacggcgg cgattatttt gtcggcggac
    12421 atatcgaagt cagtttgccg gtattacctt tgaccttcgt tcctaacgcc gaatacgttt
    12481 tcgtagataa cgcaacaagc atcaataccg gtgcggatgt gcaatataca ttcatcggcg
    12541 tcggcgtggc aaaattggtc gcgggcggcg gatatacgct caattacaac aaaccgaaag
    12601 gcagcgattc agctacggat agcggattca atttgcaatt cggcgcaaaa gccagcctcg
    12661 gtgtgagcgc tttcggactg atccgcgcta ccaatatcgg cggaaactgg accaaggcga
    12721 tggttctcgg cgtgacgttt taattttaag tgaatgcata caaggagttt tatggctgga
    12781 aagtttaaga atgtagatta ttatgatatg gatacgttgc tctccgacga agagcggatg
    12841 acgcgtgata cagttcggga ttttgtagat gacaaaatcc tgcccatcat cgaagagcat
    12901 aatcgcaacg gcacgtttcc gatgaatctc gtaaaagaaa tggccgacct cggcgttttc
    12961 ggagccaatc tgccagagaa atacggttgt gccggcatga acaatgtcgc gtacggactt
    13021 atcatgcagg agctggagcg cggtgacagc ggggtgcgca gttttgcatc ggtgcagggc
    13081 gcgctcgtaa tgtatccaat ctacgcctac ggttccgaag aacaaaaaaa ttattggtta
    13141 cccaaactgg ctaagggtga aaaaatcgga tgcttcggtc tgactgaacc tgataacggc
    13201 tccgatcccg gcggcatgaa aactaaagcc gtcaaagacg gaaagcacta tatcctcaat
    13261 ggcgccaaga tgtggatcac caacggcacg gtttccgatg tggccgttgt ctgggcgaaa
    13321 ttggatggtg tggtgcgcgg cttcctcgta gaaaaaggaa caccgggatt tacagcgccg
    13381 gaaatgaagg gtaagttttc attacgcgca tccgtgacca gcgaacttgt gcttcaggat
    13441 gttcgcattc ctgaagaaaa tattttacct aacggttccg gtctcaaagc gcccctcgga
    13501 tgtcttaccc aagcgcgcta cggtatcgcg tggggagcgc tcggcgctgc gatggcatgt
    13561 tttgattcgt ccgtacgtta cgctaaagaa cgtatccaat tcgataaacc catcggcagc
    13621 ttccagttgg tacaggaaaa attggcctat atgattactg aaatcaccaa aggccaactt
    13681 ttggtattgc aactgggtcg cctgaaagac agcaacaaga tgaaatttca tcaggtttcg
    13741 atggccaaac gcaataacgt gtatatcgcg cttgagatag cccggatagc gcgcgatctg
    13801 catggcgcta acggtatcat cgacgaatat ccgatcatac gccatatgat gaatctcgaa
    13861 tccgtgaaaa cctacgaagg tacgcacgac attcatacgt tgatcatcgg cgaaagcgtg
    13921 acgggcattc cggcttataa gtagcgctca tttcacagcc gcagcatagt tttcgtattg
    13981 catcgagatt atgctgcggc ttttttattt tcaaagtaac cgatcatcat ttcccgcgta
    14041 tattgtaaag atcaatttcg gaaatactca tgaaaatctg tcctaactgc acgaccaaca
    14101 atcttgacac gtcggcctac tgtgtcagtt gcggcgcgga actgacaata cctgttaaag
    14161 aagaacctgc gcgcgaatcc cgatttccaa aagaccacat cactcatcgc gaagatcatc
    14221 cggccagcgg tatggcaaaa ctcgccatga cgctcggatt gatttcgttg ttttttgttt
    14281 ttttcgccgt gatctgtgca ccgctacctg gcattatggc tatcatcatc ggaaacaatg
    14341 aactcaaaaa actgcgcggt gaaagatatt cgaccaacgg cgaaacatat tgtcagatcg
    14401 gcatttggtc gggttggatt tctacagcca tcggcatact gcttattgcc atcggcggga
    14461 tcgcgacgat ttggcttgtc aattggttta tcgaagcttt tacacgttaa taagtattct
    14521 tcgtattcag attttttaga ggagcagaca tgttcatcgg gcattttgcg acgggattta
    14581 tttccaaaaa atggatgccg catatttcac ttcccgtact ttttatcgcc gtacaatggc
    14641 cggacttgat atggcctgtc ctgcttttat tgggatggga atcggtacgc atcgaacccg
    14701 gcaatacagt gatgacgccg ctggcttttg atcactatcc atattcgcac agtctcatga
    14761 ccaatcttct cgttgcatct ctattcgctc tttgggcgct tcggcgatat cgcgatcaac
    14821 gcgtggccgc gttgcttttt atcacggcat gcagtcactg gattttggat tttgtaacgc
    14881 accgaccgga tttaccgctt tcgccgggca gttcgcagat gtttggcctc ggctggtgga
    14941 atattcctcc ggttgctgtc gttgcagagt tggcattaat ggctgcagga atttttatct
    15001 atcatcgcaa cactcgtgct accggaaaga caggcgtatg ggcgttttgg ggtttgattg
    15061 tttttcttat gatcattcat acaggaaatc ttctcggccc tccgccgccg aatattacag
    15121 gcatagcgct tgccggaaac ctgatgtggt tatttgtact gtgggcgtat tggatcgaaa
    15181 aaaatcgtac cgtaatcaaa taaatttcca ttatcgttta actacaggat tagtcgtatg
    15241 aaacaatggt tttgtgtttt tttgatcatc atggtcgcat catgccaaaa acctgctctg
    15301 aaacaaccgg atgtcaaaca tttggaaccg gccgcgatga ctttgatgaa acaaggtttg
    15361 aatgatcttc acgcgtacac gatgttatcc gagcttacga cgaaagcgcc taaacgactg
    15421 agcggttcta caatggcgga cagtgccgtt gcgtggggcg agcgtacaat gaaaaatctc
    15481 ggttttgaaa atgttcgctt ggaacccgta atggtaccgc attgggtacg cggacctgtc
    15541 gaagaagcct ttatcacatc aaccgccccc tctcaaaagc tcaatatctg cgcactaggc
    15601 gggagtattg ccacgcctaa agaaggcatc gagtccgaaa tcatcgaagt gaaaagtctt
    15661 caggaagtgc gtacgcttgg tgacaaagtg aaaggcaaaa ttgtatttta caatcgcccg
    15721 tttgataaaa cgttatttgc tccttttgaa gcgtacggcg gcgcggtaga tcaacgcgga
    15781 tacggtgcgg tcgaagcggc ccgtttcgga gctgccgccg tgctggttcg ctcaatgacc
    15841 aatcggattg atacctatcc gcacaccggc gccatgcgtt acaacgattc cattccgaaa
    15901 attcccgccg ctgcgatcag cacgcaggat gccaataatc tcagcgctat gctgaccaaa
    15961 ggacagtctg taaaagtgcg aatgaaactc acatgcgaaa cgttggctga taaactttcg
    16021 tacaatgtcg tcggtgaaat tcgcggaacg gaaaaaccgg aagaagtgat cgtgatcgga
    16081 ggtcatcttg acagttggga taaaggtaca ggcgcgcacg atgacggcgc cggggtcgta
    16141 cactctatcg aagcgttgcg attgatgaaa gcaaccggcc ttttacccaa acgcaccgtt
    16201 cgtgtcgtat tatttatgaa cgaagaaaac ggcgtccgcg gcggtaaggg ttatgcggcg
    16261 gttgagcgac caaacgaaaa acatattgcc gctttggaat cggacggcgg cgggcatgca
    16321 ccgcgcggct ttggcataga agctgattcc gtaaaactac cgcggattac acgatttgaa
    16381 tcgctcctca aaatcataga tgccgatcgc gtcgggcccg ggcacggcgg aaccgatatc
    16441 gaacctttgg gtaaaacggg cgtaccgtgt atcggacttc atcccgatcc ccaacgttat
    16501 tttgattacc atcattccga tcacgatacg atcgacaaag tacatgatcg cgaacttgaa
    16561 ctcggagcca ttgccatggc gattctaact tacgccatcg cgtttgaagg catttaaaaa
    16621 acttgcagta tcaaaaagaa gaaactacca cgatatgcct aaggtcgctt ctatcacgtg
    16681 aaacccgtaa cagaaattaa tacggttcat ttgtttcccg agctggaccg attgctcatc
    16741 gcatgcctgc gaaatctaag gccggaagac tggcgtgtac agacatcagc cggttcgtgg
    16801 acggttcgtg atgtagccct acatctgctt gacggtaata ttcgtttagt atcagcgatg
    16861 cgcgaccatc attttgcacc ggcaccgaac gatctttctt cgtatgaagc aacggtacag
    16921 ttccttaata cactcaatgc cgactggctc aaggcttttg agagagtcag tccggcgatg
    16981 atatgcgaat tactcgaaat tacgggcgcg tggtactgtg cctatttgaa aacgatacgc
    17041 cataatgatg atgccgtatt tcccgtcgcg tgggcgggtg aatccgtttc taaaaattgg
    17101 tttcatgtcg ctcgggagta cactgaaaaa tggcatcatc agcaacaaat tcgttcatcg
    17161 cttggtgatg aggagacact tttgcagaac gcatggtatt tgccatatct tgataccagt
    17221 atgcgtgcgc tgccgcatgt atatcgtaat atgccggctc ccgacggcat agttttacag
    17281 ttttcagtat ccgacttgac tgatgctaat tggtttttga aaagaaataa taacaagtgg
    17341 gaacttttca gtggtactga ttcgaagcca cacgcacaca tgcgtatcaa aaaaaaagcg
    17401 gcttggcgta tttttactaa aaatctgaat ggcgagcgcg cacgtccata tatccatatg
    17461 cttgacggtg atgaattttg gcttaacccc ttgctgaata tggtggctgt catggcttga
    17521 tttttttata accgacagtc aataacttat ttatacaatc aaatgaaacc tatgattatt
    17581 gcagaaacgg atcgtttgat cctccgccga ttgacccatg aagataaaca ttttatcctg
    17641 cgattggtca acgaaccatc gtggttacgg tttatcggag atcgtaatgt gcacagcccc
    17701 gaagacgctg tacgttatat taccgatggc ccgatggcga tgtatgccaa atacggattt
    17761 ggtttatttc ttgtagctct aaaagggtcg gatacaccta tcggcatgtg cggtttgctc
    17821 aaacgtgaca cactcgaaca tgcggacatc gggtttgctt ttttaccgga atattggggg
    17881 caaggttatg cgtacgaagc ggcggcggct acgatggctt atggtcaaaa acagcacggc
    17941 ctcaataaaa tcatcgccat cacctcgccg gataatgctt cttctatcaa actgctcaaa
    18001 aaactcggcc tcgtcgaaaa agaaatcatc caattgcccg gctacagcgg cgaaactttg
    18061 gtcatggaat aaaaaaagag ccgcaattag cggctctttt ttatagtaaa tagaattaat
    18121 caaaattcta catcgccgat gcattcaata tttttgcagt tttttcctga aaacggcgtt
    18181 ttctatcttc gcgtgtacga cgctcctgta catgcgagga cgatttttta acattggagc
    18241 cggcttctat accgacggca aaagacatat agaagttgtc atccgaaata atatgccaca
    18301 taaaccctga tccatcccca ttcatattac cggacgagcc ggctttagcg ataggtccat
    18361 tggcgcaatc aataccgtaa atatataagt acgactccgt atcaagcatg ctggccggaa
    18421 aagtaaaggt attaccctca aatgctgaat ccatttcaac atagtaccat gattcgccat
    18481 cataatattc taactctgcg gaaatatatt tgaagtctga gtttccgcca tacgtcgtca
    18541 atgtcagcgc ggtattcaaa ggaatataat aatctgtcgt acttgctttg acactatcat
    18601 acatcgtcgg ctgatctacg gtacctgtta tcgttccttc ggtagttttg atcgtgatat
    18661 ctccatcgcc aaatgttgcg tcgtacaaaa agtcaaaatc gaatgaattt tgagaaacat
    18721 ataactcttg cacgccggtg atgttttttg cagtaaccga ttcgataaac ggcagaggat
    18781 cacccatgac gattccgtca tagtcaaaat catttccttc ggagcccaaa tactcatgct
    18841 gatatacata agcgataagc gtattggaat tattgctgtc gcaccccaac atggtcagcg
    18901 tcagaacacc taacacaagt aatctcatac ttttcataaa atcccctctg gttttgttat
    18961 acaggttaat tgttgatgac acctgtaatt acgattgaat agcttttagg aaatcaaggt
    19021 aaatatattt tataatgatt acgatgtcca tctcttttat gtgtttataa aaaccgcttg
    19081 atttccataa cgataattgt ttttattgat cagccatgta tcataaagtg taccgttgat
    19141 tgaaaaaata tcccgtcctt tctctgcgcg aacgcgtccg gccgcatacc gactatgatg
    19201 gtagcggtgt gactatagct tttgtggaca gcggatatta tccacatccg gaattgcgcg
    19261 gtcgtattct gaaatttgtg gatgtgtcat cggaagcgtt tacggaaaaa gatttccaca
    19321 caatccggcc gtccagttgg cacggtacga tggtagcctg ctccgcctgc ggaaacggca
    19381 tctggtccaa aggttattat cgcggaatcg catccggagc taatgtcgtt ttgatcaaag
    19441 cctttgacgg taaaaaagta aaatcatcgt atatccttaa atccctcaca tggattatcg
    19501 cccatcacaa gccgtatcat attcgcgtcg tcagtgtatc cgtgggtggt gaccgcaatg
    19561 aaccttccga aaaaagccgc atttgcaagg ctattcaccg tttacatcag ttaggcatta
    19621 ccgtgattgc ggcggccggc aataatcctt cacgaccggt gattccaccg gcgagctgcc
    19681 gctttgctat cgccgtcgga ggtatctacg atcataacga caagaacccg gatacggcca
    19741 gcgaatatgg ttcctcctac ggcataactt tagatggtct gagtaaaccg gaaatcgtag
    19801 cgcatgcccg gtttttaccg gcacccatgc tgccggataa ttttacatat gaggaatcgc
    19861 aaattctgca tgtactttat aaaactccag ccaataatct gaagcgtaag ttaaggtcgt
    19921 tgatacataa aacgcgttta gacgcggcta ttctttctct caacccggca gaaatcaaaa
    19981 aagctatacg gcaacgcatg gacggcgaaa aatttatcgc accgggtttt caacatgttg
    20041 atggcacttc atttgccgct ccgatcatag ccgccgtagt ggcgcaaatg ctggaagcta
    20101 atccattgct gacgccgacc atgatacgta tgatcctcga aacgacggcc aaacgtgtac
    20161 ccggtattgc accggaaaaa caaggattcg gcctcgtaca agctaaggct tgtgtcgaac
    20221 aggctttgat agaaacgtct atgccggaaa ttcacgagtc tccattgata ctcaaagatc
    20281 gcattgtttt tttgtattac catcgcaata tccgcaaggt caatcttgtc ggcgatttta
    20341 cggattggca aaagaacaaa ttactcctcg ttgaaattcg tcccggtgta tggcgtgttg
    20401 atatacctct tttatcagcc gggcaatacc actacaaatt ttttgtggat gataccttat
    20461 ggaccgaaga cccgctcaac ccgcataaaa tttccgaccc gttcggagga atgaataaca
    20521 ttctgcatat ctgatcgtct gtccttcata caaatgcccg ccaagtgcct ctttttcctt
    20581 gcaggcttgt attttatttt cactcctgtg aattaatctt tcgaatcgca catcaataaa
    20641 gcattaattt ttaaggttat gtttttggca tacaaattgt gatctctatc acacgtagca
    20701 ataacatatc aaaaaccttt agggagtctc gtcatgaaaa atttactgaa aatcttcaca
    20761 ttcgttctcg cgaccaatgc aatggtgacc gcccaaacga tcgaagtcgc aaacgaagaa
    20821 ccacttacgc ccgagccaaa aagcacttac tatgaaacca aaaaccgtat cggctttgct
    20881 gcaggaaatg tatccggggt tggcttatca tacgaacgtg atctttttag aaatttctcg
    20941 gcgctgtttg tcatcggcgg tgttgcccaa aaaagtcaaa gcgattttaa taccggcctt
    21001 actttcaaat acacttttag ccgaatcaac aaacgtttca gtgtcaatgt cgtcagcggt
    21061 ggcagctatt ttctggatat agatgatctc ggtgtttacg ataatacaaa cgagaatcgt
    21121 agcttagaac gtcgtcgcag ttatatcaaa atgggattgg ggctaggaat gacggcgtta
    21181 tttttcgacg gacgtttggg tattgatgtc aattttatcg gtatgggcgc cagctttcag
    21241 aaaaaacaaa acaagaaaga ctatcagctt ggcgataagc ccatccgcgg gcctcaagtc
    21301 aatctgacgt atcactttta attccgatat cgttttcacg ggtcctgtcg tgccaagggg
    21361 gtaacatggg aatctgaatc ttttagaaaa cgtatcggga gcatcggatc ctctccggta
    21421 tttaggctga aaacaaatgt gttctcagcc ttttgccgtc atatgtccga ataatcaacg
    21481 aactgaaaaa aatcttgaca tcctcggtct cttttgatat tattgcctgc ctgaaaataa
    21541 aaaaacatat gaatttggct ctgtagctca gttggtagag cagaggactg aaaatccttg
    21601 tgtcggcggt tcaattccgt ccggagccac agcaaagacc cttaagttac aacagcttaa
    21661 gggtttttag tttttactgc ttatcccctg atttagctca aaaaagcaac caatggtaac
    21721 cattttggta accacttttc cgaagcgttg gaaagggtta tcatgtttct ttcaaagcat
    21781 cctaacggca cgtattatct ttggtacgtg gacgaaaaca acaaaaagca gaagatttcc
    21841 acacgaagca aaaccaaagc cgatgctctg aaatttctca aagatttcaa tctcgaaaag
    21901 taccggcaaa gcgaacgtat ccgatgtatt cacttatccg aatttacctg cgattacgct
    21961 aactattcca aggccaatca ccgaccgacc acgacgcgca cggccatcac ggcacttaag
    22021 gaattgagcc gaaaaatggg aaaccctctt ctatcacgta tttcggttca tgatatagaa
    22081 agcttcttca cgtcaaaggc ccaagagtgc aattcgtggg ctctgctaaa gtattacgtc
    22141 accttaaaaa gcatatttaa cactgccaaa cgatgggaat acattaccgt caatccattt
    22201 tcaaaaatcc ccaaacccag accggcagaa caaacacccg tttattttac acatgctgat
    22261 tttaccaaac ttttgagagc aatagatgat tcggatttca aagaactcgt tctttttgca
    22321 ctgatgagcg gtatgcgcca gagcgaaatc gtacatctca catggagtca aattcatttt
    22381 gagaaacgtc tcatacagat acgcaatacg gaatgcttta cgaccaaaag cggaaaaaac
    22441 cggaatgtac cgattggaga agaactacta atactcttag aaatgaaata tcaaaacacg
    22501 atggagaaac acagctacat atttcaacgc aacgggaaac cctaccggca cgaatatgta
    22561 tctaaaaaac tcaaacaata catccgaaag acggaattgg atgaccgact gcactttcac
    22621 agcctcaggc atagttttat cacgtgggct ttagagcgcg gcgcaagtct tcatcaagtt
    22681 tccaaggtag ccggacattc aagtaccgcg ataactgaaa aagtctatgg tcacatcaaa
    22741 gccgaagaat tgcatcatgt cgccaacctc ataacgataa acaaataatg tatcggctta
    22801 aactacacta tggcaataag gacaattgta cacctttttg tatctttgta tctaatcata
    22861 gtataagcca atggtataaa tattaaattg actgatagtc tttaattcgg taacattgac
    22921 tattcatttc taatcgtttt tagcgaaaaa agtaatccgg ctttatcgag aattatttgc
    22981 taaactacca ctttcgaaaa accaatgact attcaatcgc atcgtgaaaa agccaacttc
    23041 atctggcaaa tcgccgacct gctccgcggt aactataaac aatccgaata cggcaaaatt
    23101 attcttcctt tgactgtact ccgtcgtctg gaccaaattc ttgagccaac caaggccaag
    23161 gttttacagg tctatgacga caataaaaaa cagaagccgg aaatcctcga aaagcttctc
    23221 aataaggccg ctaagcagaa atttcataac cgcagtaagt tcgatttcaa aaaactggcc
    23281 gctgacccca atggccttga aaaaaacctc cgcaattata tcaacggctt ctcagccggg
    23341 gccaaggaaa tcttcgatta tttccaattt gaagatgaaa tcgaaaaatt agacaaagaa
    23401 aacctgcttt atcttgtgat taaggatttt gccaaacagg atttgtccga cctcgatacg
    23461 atgcagatgg gctacgtatt cgaggagctc attcgcaagt tttccgaatt atccaacgaa
    23521 accgccggag agcacttcac accgcgggaa gttatccggt taatggttga tttgcttttt
    23581 atcggcgata acgacatcct ccgcaaggaa ggcattatta gaacgctgta tgaccctgca
    23641 tgtgggacgg gtggcatgct gtcggtcgcg gaggcctatg tacaggaact caatcctaat
    23701 gcacggctgg aagtatttgg gcaggaaatc aacgacgaat cctacgcaat ctgtaaaggt
    23761 gacatgctca tcaaaggcca gaatcccggc aatatcaaat acggcgacac gtttaccaaa
    23821 gacggacttg aaaacgaact ctttgattat atgctctgca atccgccctt tggcgtggaa
    23881 tggaaaaagc agaaggaagt catagaaaaa gaacatgacg accgcggttt cgccggacgg
    23941 ttcgggccgg gcctgccgcg tatcaatgac ggttcattgc tgtttcttct gcacatgatt
    24001 tccaaaatga aaaaggacgg ctcgcgtatc gggattgtct tcaatggctc cccgctattt
    24061 acaggttcag ccgggagcgg tgagagcgag attcgtaaat ggattatcga aaacgatatg
    24121 ctggaagcta tcgtcgccat gcctgaccag cttttttata ataccggcat cgcaacgtac
    24181 gtatggattc tgaccaaccg aaaagctaaa aaacgcaaag gcaaagtcca gcttatcaat
    24241 gccgtctcat tcttcgaaaa aatgacacgc agtctcggca ataaacgcaa tcttatcagc
    24301 gaaacgcaac tgacgtccat cgctaaaatc tatggcagtt tcaaatccgg cgaattttgc
    24361 aaaatctttg acaatgaaga ctttggctac gcgcgtatca ccgtagagcg tccggccaaa
    24421 gatgaaaagg gccaagtcct caccgataag aacggtaacc ccaaagccga ttccgaactc
    24481 cgtgattatg agaatgttcc gcttacagaa aagatagaaa cttatttcaa gcgcgaagtg
    24541 ctcccgcacg tccctgacgc atgggtggac gagagcaaga ccaagattgg ctatgagata
    24601 aactttacca agtatttcta ccagtacaag cccttgcgct ctctgtcgga aatccgagcc
    24661 gatatacttg cgctgaagaa agagacggaa ggtatgatta gggaggtcat aaaataaaaa
    24721 aacccggctt catgcaccgg catcgaaaca ccgggagagg agggaccggg actgcttacg
    24781 caataattat aagtattatg aacccagaaa tcaagataga aggataaaat ggataaagca
    24841 tggtttcaaa aggtgttttc tgaggaacga actcaaaagt attttgagac ttatccggcc
    24901 aatgacaaga aagcgattga gcattatgca tgtaatatca tgatatctga agctttttat
    24961 ccggcattgt ctatttttga agttgcatta cgtaatagcg ttaaccgcga gctcgaaacc
    25021 atgtttcata ctcaagactg gtatcagcaa attcctacaa cagccggtct tgtaaaactc
    25081 aataaagata ttagtaatgc aaaacgtcaa ataatgatgc gcaatgaagc catcaccgct
    25141 tctaaagtgg tcgctgaact tacattggga ttttgggtgc gtttattcaa tacggagtac
    25201 gaacgcattt tatggaaaaa cttacgcctc gcttttccct atctggaaaa gagaaaacgt
    25261 cagcgtcata ctgtatctgc gccactcaat cgcatccgtg atttcaggaa tcgagttttt
    25321 cacaatgagc cagtctgttg gaatatcaac agtcttgaga acaaccacaa aaccatagtc
    25381 gaaacactct tttggctgaa taaagacttg cctgactacc tcaacactac agaacgattt
    25441 catactgtac taaatgaagt acgcaaaata ttatcatgaa agcctacccg aaatacaaac
    25501 cctccggtgt ggagtggatt ggagagattc cagagaaatg ggagctaacg cgtatgaagt
    25561 acgttgcccc actccaaggt ggttatgcct tcaagactga agcatttaag acagaaggaa
    25621 tcgctattgt aaaaatgaac aacctaaaaa gaggacgctt ggatttaagc gatgcggtgc
    25681 gtgttgattt taaagcggca gtagatgctt tttctctaaa agcaggtgac cttctttttg
    25741 gaatgtcagg aagtttgggt gaaaccggta gtttagggaa ttttgcggtt gtgaacttag
    25801 ttgacatacc ttgccaacta aatcagcgtg tcggtagatt tgttgcgtta cagaattgca
    25861 gcttggattt tgttcggttt ctcatcacat cagtaagttt tactgagccc ataatgtctt
    25921 catcaacagg aacagctcaa ttcaatataa gttccgagca aattggaaac attttgattt
    25981 cactcccccc tctccccgaa caaaccgcca tcgccgactt cctcgaccgc aagaccgcgc
    26041 agattgacgg cttcatcgcc aagaaacaaa agctcatcga gcttctccgc gaggaacggg
    26101 ttgctgtcat caatcacgcc gtcaccaagg gcatcgaccc caaagccaaa atgaaaccct
    26161 ccgggattga ttggttaggg gagattccgg cgcattggga ggtgaagagg ttaaagttct
    26221 ttgccaatgt taatccaact aaatgcaatt atgtatttga taagtcatcc aacgagttgg
    26281 tggtattttt gccaatggag aacgttttag aggatggaca aattatccaa gacttgagaa
    26341 agcctataaa tgaattgagt actggttaca cttatttcga aaaaaatgat gttatcgttg
    26401 ccaagattac accttgcttt gagaatggaa aaggtgcttt gttggacagg ttggaaacgc
    26461 cctttggctt cggctcaacg gaatttcaca ctataagacc cgtcaaagct gtttccacga
    26521 acgaattttt atacttcctt accaagactg atttgtttat gaaatttggt gaggctttta
    26581 tggtcggggc cgctgggcaa aaaagggttc cgcccaattt tgtagaaaat ttcatgattg
    26641 cctcgccttc ccttgacgag caaaatcaca ttgtagcttt tctaaaatcc gaaaccgcca
    26701 aaatcgaccg gactatcgcg accatcgaga aggaaatcga gcttatacag gaataccgga
    26761 cggcgttgat ttcggaagct gtgacgggta agattgatgt gaggggttga tgtgaccctc
    26821 cgctataagt cacataaaaa caaaagcttt gctgacgagg ttacttttac ctgcacatat
    26881 aaaaatttat atttgcattt aaagcttttt ctgcaattat aattttttat atttgcagaa
    26941 gcacttttca aatgaacttg gaaaattatg gatattaaag attttaaatc cggtatcttt
    27001 cgccaacaat atgaatataa aagcttccta cctaactcga ttcatcgtga atgggtcgta
    27061 tcggatgcac gtctcaataa ccttttaagc gaagctaatc tcacattggg acgattggat
    27121 gcattttcac ggcttattcc cgatgtggac ttctttattc gcatgcatat tgtaaaagag
    27181 gccacacaaa gtagccgcat agaaggcact caaacgacca tggaagaagc gtttttaaaa
    27241 gaagcggatg ttaatcctga aaaaaagaac gattgggcgg aggttaataa ttacatcttt
    27301 gcactgaagt atgccattgg agaattgaaa aaactgccac tatctacgcg cctccttcga
    27361 caaacccatg aagtactcat gcaaggcgtc cgcggccgtc ataaaatgcc cggtgaattt
    27421 cgttcgagtc agaattggat tggcggtaca tcgcttaaag atgccatctt tattccgcct
    27481 attcatacgg aagtcccgga gcttatgagt gacctcgaaa aattcctaaa tgaaacacgt
    27541 cttcaggtac ctcatctgat taaaatcgga ctcgcccatt atcagtttga aaccatacat
    27601 cccttcttgg acgggaacgg gcgacttgga cgccttctca ttacattgta tctggttcga
    27661 tttggaattc tcaacgggcc aaccttatat ctatctgatt tttttgaacg caatcgttcg
    27721 ttatactatg ataatctgat gcaaacacgc ctccagcaca atctccctca ctggttaaca
    27781 tttttcctga ccggcgtgtt acagactgcg acgaactcta ttgaaacctt tgaaaaaata
    27841 ttaaagttgc aggaagatat agagggaaaa agaatactta aactcggaaa aaagagtgaa
    27901 gcggcaaaaa cattggtcaa acacctttac aaaaatcctc ttgttaccgc ctctgacgtt
    27961 gaaaaaatat tacacgtaaa tatttcaacg gcgaaccgta tgattgatga ctttgtaaag
    28021 ttgaaaatcc tcaaggaacg aaccgggttc aaacgcaacc ggattttttc ttttcatgaa
    28081 taccttggac tattttataa tcctcgtaaa tgattaacac cgaaaaaatc ttcgaatcag
    28141 ccatcgaaga ccaccttctg gaatctggct ggctcaaagg ccgtgcggaa gactttagtc
    28201 gcgaccacgc gctcgatgcc gggcaggtta tcggttttct caaagacagc cagccaaaag
    28261 actgggaaaa aatagctaaa attcaccagt ctgatgttga cactaaggtc gttaatcgtt
    28321 tatccaagga actggaactc cggggaacgc tggatgtttt gcgctacgga tttacggata
    28381 gcggcgtcag gttccgcatg gcctatttca ggccggaaac aggtctcaat cccgatacgc
    28441 ttgcgctgta tgcaaagaac cgcctcatcg ttacccgtca ggtcaagtac agccccaaga
    28501 acgaaaattc gattgattta ttgcttagca tcaacggcct tccggttgcg accgccgaac
    28561 tcaaaaatcc tttcacgggg cagaccttca aaaatgctat ccgccaatat caacatgacc
    28621 gcgacccgcg tgaattgatt ttccaattca aaaaacgggc tttggttcat tttgcggttg
    28681 acccggatga agttcacctg accaccaagc tcgacggctc gcattcacga tttctaccat
    28741 tcaacaaggg ccataataac ggggcgggaa atccgcccaa tccggcaggt tataaaacag
    28801 cctatctatg ggaagaagtc tggcaaaaag acagttggat ggacattatt taccgattca
    28861 tgcatttgca agtcgaagaa gtcaaggtcg gtagaaaacg cgagaaaaag gaatccctga
    28921 tattcccccg ctttcaccag cttgagtccg tccgcgactt gaccaacgac gcgcgtaaga
    28981 acgggccggg aaaaaattac ctgattcagc attccgcagg aagcggaaaa agcaactcga
    29041 ttgcatggct cgcctatcac ttgtccagcc ttcatgatgc caccgataaa cgaatctttg
    29101 attctgtcat cgtagtaagt gaccgcacgg tgcttgacca acagttacag gatacgattt
    29161 accaatttga ccataaatcc ggcgttgttc agaaaatcga cgagaattct aaccaactcg
    29221 cggaatccat caaagcagga actccgattg tcattacaac gttgcaaaaa tttcccttca
    29281 ttctcgacaa agcaaaagct ttgccgggcc gtaactatgc ggtcatcgtg gatgaagccc
    29341 acagctcaca aggcggcgaa tcctccaaaa agatgaagga agttttgtcg gctcaatccc
    29401 ttgaagacgc agagaaggaa gaaggggcag aaacagacga tggagaagat gaaattcgaa
    29461 aatctatggc cgcacggggc agacaatcta atctaagttt ctttgcattc acagctacac
    29521 ccaaggcaaa gacgcttgaa gttttcggaa ctaagggagc cgacggcaaa ccgaggcctt
    29581 ttcatctcta cagcatgcgg caagccatcg aagaagaatt tattctcgat gttctgaaaa
    29641 actacgtcac ctacaaaact tttttcaaac tctctaaagc catcgaggac gacccggaac
    29701 tgaataagaa aaaagccgcg attgcgatag gccgttttgt ttctttgcat ccctataatc
    29761 ttgctcagaa aacagaaatc atggtcgagc atttccggca agtcacgtct aaaaaaatcg
    29821 gcggaaaagc caaagcgatg gtagtcacag gttcccgcct acacgccata cgctattatt
    29881 tcgaatttca acgatatatc aaggaaaaaa gctatagcga aatcaaagcg ttggtcgcat
    29941 tctccggtaa ggttatcgac acgggttatc cagaaggcgt ttcggaatct gaactcaacg
    30001 gttttggcga aaaagaacta cccgataaat ttgaaacgga cgagtatcaa gtattgctcg
    30061 ttgccgacaa gtaccaaaca ggatttgacc agccgctatt acatacgatg tacgttgata
    30121 aaaagctctc cggtgtgaag gcggtacaaa cattatcaag gttaaatagg acatgcgcgg
    30181 gcaaagagga tacattcatt ttggatttcg ccaacgacgt tgagacgatt ctcgaatcgt
    30241 tccagcctta ctacgaattg accacaatgg agcaaacgac cgaccctaac ctactttatg
    30301 acctccgcca tcggctcgac gaagcacaag tttacttgca atctgaaatt gatgcgcttg
    30361 cgaaagtgtt ttacaatccc aacggctcca ccaaagacca agcccgtctc aatgccttgg
    30421 ttgaccctgc ggttgaccga tatgaagata ttgatgccga cgaaaagaag gatgaattca
    30481 agaaatcgct tacggcattt gtccggctct atgcgttcct gtcacaggtc atgccattca
    30541 gcgacatcga attggaaaaa ttttacgtgt acgcgcgatt gttagtatcg aaaattcagt
    30601 ccccagatta tggcggtagg ctgaaactgg ataacgacgt tgctttgcag tattaccgtc
    30661 ttcaaaaaac ttctgagcac ttgatggtgc tggaaaaact acctgaatac tcgctcgacc
    30721 ctacgactga ggctggcatc atgcgcgaaa aagaaatgaa agccaagctt tcggaaatca
    30781 ttgaaatttt gaatacccgc tttggcatgg atttggatga agcagacaaa ttattcttcg
    30841 accaaatcga agtagagcta cttcaagacg agaccctccg ccaacaagcc gtcaccaatt
    30901 ccatcgacaa cttccgttac ggtttccaag aaaagctcga cagtacgatg ctcgaccgaa
    30961 tggaaagcaa tcaggaaatc ttccaaaagg tcatggaaaa cgacgaaatc aaaaccgccg
    31021 tactggaagc catgataaaa caggtgtacg cgaagtttaa caacaaaaac cttcagtaat
    31081 ccaccccccc ctcaataact attaatactc caaaatgcgc tcctaagtgc atttcgcttt
    31141 gcaaaggact tcaactgatt tatattcagt taagtaccaa tacgctacaa tataaatttt
    31201 atacttccgc attgtctgat gacctgtagc gggtccgtac cggatgggtg cagaattttt
    31261 gttttaaatc tgaatgggca aaaaacaaaa gaaatcaaaa ccgagggctg attcaggcat
    31321 tcaaatgagg tcgattaata tcgaagacct taaccataaa tacggttatg attcgaaatc
    31381 aacaaagcct gttaaaggtt ttgattctat tgagtattca atgtatcgca ctcgattaat
    31441 tggccacgta gacggcggta ttttcaatga ggttaacggt ttatacagcc aaaaacgctt
    31501 ttgggcttat ttaacaaagg ttttaaaacc caacgcccaa aggattgctt tttttaagga
    31561 tttaatgaaa ggaatcccga atggaacagc cctaaaatca caattcgatt cagctttcaa
    31621 aaacttctgg aagtccctcc gtcaagaaga aatcgagata gaacttcaag gcgataccat
    31681 ttctttacca tcttatttga atttgctcct tttcgatgaa ttcaagaaaa cttattatca
    31741 cagagtattt tctttaattg ttgaaaacac tgtttttttt gacttaaaaa taaagccctc
    31801 taaggaagcc ggtaaagttt tatctgaatt ggcaaaatct ctagcaaatg aaatcaagag
    31861 agggctgtca cttgaactcg aaggtttttt tggttatcaa attcgattgg caaaaattgc
    31921 cgttggcgac cctcaacaaa tcaaacgtga attcgaaaaa ttgatgcgat acaatcaaaa
    31981 agttaaggtt tactgcgaag aaaaacactt tcccaaaggc gtcctgcttc cgattgaagt
    32041 gcgatacaat ctcgtgaagc acctcaagaa ggtcacttat ccgtccagcg agttcagttc
    32101 aaaaggccaa ggcgcgaaag atatgattct aattaataaa attctcaatg aattaggata
    32161 cactacaaaa cgaaggtcac attcttgatg attatacttt agaagtataa ttatacttct
    32221 aagtatatct cttcccgggt aataaatttt gtccatgaaa aatgggcaaa ataaattttt
    32281 actcagtgta aaaacagccg ctgcgaggtt atgcccattt cctccgtcgg ctgttttgct
    32341 tttaaaagag aacgggccag agcgtcaact ctgacccgtt cggaaagtac tttatgcata
    32401 acaaacctaa cccttccacg gtcaaaagtc aaatccacat ccatccaagt gggcacgtct
    32461 atcttgctac tgaaaccaga aagcgattta tcggaaaaat cattactcgt gaatttcact
    32521 gtgagcgaga cccggagcgt cacattcttc gagtaaacaa ttcgattgga tttaatcttg
    32581 agttgatgcg gatgtcggag cagttcgatt gggttatcat taccggtcta agttctcagc
    32641 caattattgt tacgcgtgac ttcattcttc gtaaaggatt tcttccttca tttaaaaatc
    32701 aaagctacga gttacaaatc tttgtaaaac tcaatgacct gaagaaagct gatacggagc
    32761 cggatttatt ttctccggag gccgcgtaat gaccacagaa gacatgttag acatttttaa
    32821 agggcgtgtt gattttgtcg gcacgtcctc cggaatgtct gtacaagtaa accggccctt
    32881 ggttgactat gttcaaagac atcttgacgg cgttgaccgc ttaggttttt ataacttact
    32941 acccaataat gtaattcgct ttgcggtcat tgattttgat tcccatcaaa agccggatgc
    33001 atatcggcag actatggaca aactcagtag tcaatgcgaa gaatatctca gaaaagcagg
    33061 tctctatgtg tatcgtgaaa tatccaaaag cggctacgga aattatcatc tatggatttt
    33121 ttttgatgaa gatatttcag caaaattcat tcggtcagta ctatcagcat tcattattca
    33181 ggttctaaaa cctgaaaccg ctcttgaaat atttccaaaa caaaatgccg gagataaagg
    33241 aaacttcatt tggctgccct ttttcggaaa agatgtttcg gagaacaaga cggtttttat
    33301 tgacgcatca ggaaaaaaat acgatgctga atttaaaccc aactcccgca aaactttaga
    33361 tgaattggcc gatttctatc atcttgagtt aaaggaaact acttcggcaa aagtgtcgag
    33421 aaaaacagat tattcggaga tattaagcaa tggcgttccg gtgggcgaaa gaaatagcac
    33481 cgtaacacgt attgcgggtc atttgaagca tgctggcctt cctattagcg agacgaaagc
    33541 tattcttcag ctttggaaca aaaacttagc cagtccccta tccgataaag aactatgcac
    33601 gacggtgcgc tctatttaca gctattcaac cgaaagcgca caagatacta tatatcacaa
    33661 aggcggagcg atgcttaaaa tgcctatccc agttcattac gaccttgtag aaggtctaat
    33721 gcacgagcag tcagtcaatt tcttatccgg tgaagaagga tgcgggaaat cgcttttagc
    33781 aatgaatctg gccatttctg tggcgtcaga taaacccaat tttttgggtt ataacatcac
    33841 aaagcacgga aaagtgcttt tcttaaataa tgaacttggg tttccagatt ttctgattcg
    33901 tttccaaaaa atgtccgtcg ggctgaatcc agagcagttg gataatttta ttacaccgac
    33961 tacagttccc cctctcaatg aatattattc agagcttgac aaactgcttg ccgctgaaaa
    34021 accgtgttta cttgtactgg actgtcttta ttggtcgcat gacttgaaag aaaacgattc
    34081 gagtgaaatg aagtcactaa tgcggcagtt aatatcttta agagacaagc accggctttg
    34141 cattttggta gtacatcata caaaaaaagg ttctcggtat caaaagatgc acaatgacaa
    34201 tatgcgcggc gcgggcgtat tcggctctgc tacagacagc gtatttcaat ttcgccgttc
    34261 ggccatagac gacacaaaac gtttattcaa gccgaccaaa ctacgacacg gggccgatgg
    34321 ccttcgacat gcacacctaa tcgcattaaa cgatgaaacg ctttggtttt gcgatgaagg
    34381 taaaaccgat gaagatgaac acatcgcaac attaacaacc ccaaaaaagg acatatttga
    34441 gattgacttc tcagccatca tgaagccggg tgaaaaagtt tccagaaagg agattttaga
    34501 aaaatgtaag tcgcttggat gcgatgaacg aacaattgac agaaaactca aggaagcaaa
    34561 agaccgtagc ataatcgcat ccgttaagaa tggtcaatat actttgacat gaatatcgta
    34621 ataaaaacac tgtcaatgtc aaagtcagat agaactattt aagagtttga catagacata
    34681 gattttccta tgacgccgtg tcaaacttcc agggaggtca aatattgact tatgcatata
    34741 aacatgcaga ttttaaattc aataaccacc cgctcttttt ttttggcata gggggtatat
    34801 caattttaaa ttctaacact attactaata ctcgtcaata acacacaaat accatactgg
    34861 aacttttttc ttttatactg cgttaaagca agcgtgcata actaccacac aaaataaaat
    34921 tgtaaatcga gaaaccgtta ggcgtaatag gatgtggtag tccttgcgca cgcttagcgg
    34981 ttttttcgtt tgttatttga caattcggaa atataggtaa ccaaaaaggt aaccactggt
    35041 ttagttaagt attgtaactc attgtttgct ctgtagctca gttggtagag cagaggactg
    35101 aaaatccttg tgtcggcggt tcaattccgt ccggagccac agcaaagacc cttaagttac
    35161 aacagcttaa gggtttttag tttatgtcct atgacattat ttcacatggt cttatttttt
    35221 gtgaactagc gtatggctat aaccgattgc gtggtgatat tttttcattt cttgaactta
    35281 tgggtcattt ttttgtaagt tatatgcaat tgtgaccggg cacgtcccat gcctcacatc
    35341 ggtttcaatg ttcgtgcttt ccggttaccc ctaccttttt tgaaactgcc gacgctatta
    35401 cgaaagtaaa gcttcatgag tatcacttct gaaaaaatat tccgccttct gttttgtgtt
    35461 ttcctttttc ttttcatgtt ggctgccgat acgaaggcgc tggatccgga gcgcagcgta
    35521 acgcaatacg tgcacgatgt ttggttgaaa gaagacggtt tacccggaat ctccgtgctg
    35581 tcgttttatc aaacccgtga agggtattta tggatcggtt cgtttgaagg gttggcgcgt
    35641 tttgacggcg caaaatttac gcttttcaat cgccgttcca ttgatgcttt tctcagttac
    35701 gggctctggc gaatcttaga agataaacac ggttatattt ggatgggaac caacggcaac
    35761 ggtatttatc gctttaaaga tcctaacttc caacgccttg ccgtaaccaa cgggctcacc
    35821 aatgacaatg ttttatcact ggcgatggat caggatgaaa tggtttgggc gggcacccga
    35881 aagggattaa acagaatacg aattatttca caagatagtt ttgccgttga gcccgtgatg
    35941 tatcgcagca atgtactgca ggatgttttt gggttgtgca ccgatcacga cggtaccgta
    36001 tggataggcg ctacaaatac tatttatcgt tatgtcgaag gtcgccttga ggagtttgca
    36061 aacatcggcg agcgcatccg ttcgatgtat gtagatcgcg ataataggat atggatcggc
    36121 accaccaacg ccggcgtttt tcaaatttca gaaaatcaac tgcgccattt tacaactaaa
    36181 aacgggctcc ccccggaatc catctggcgt gtgtggtatg accgtgacgg caatgtttgg
    36241 ttgggttcgg atggtatggg tctttttcgc gccaaacccg gaagtttccg cgacgaaaac
    36301 gaattgattt cccttgagca gttttcagaa aaagaaggat taaccaataa tcgaatttcg
    36361 gcgctttttg aggatcggga aggcagttta tggatcggca catttcgcgg tggtctcaat
    36421 cgcctgcgtg acggaaaatt tatcacttat gcatccgcca acggaatggt gcacccggtt
    36481 gtttatccca tcgtacagga acataacggc gatatgtggt ttggtacgga gggcggcttg
    36541 tcgcactatt cgggcaaccg ttttgtaaat tacacgacgg ccaacgggct ttccaataat
    36601 caggtacgcg gcattgttcg tgattccagg ggccgcctgt gggtcggtac gtacggtgcg
    36661 ggtttagaca tttttaatcc ggccagcgga cgattcgcgc cggctccgtc tgcgattaac
    36721 cacgcctttc gcaacattcg tgatatgctt aaagaccgcg aaggccatct gtggatcggc
    36781 actatcaaca gtatcggatt gatcaaacca gacggcagcc accgtacttt taccatgcaa
    36841 gacggcctgc ttaccacatc tattttatgt ttgtacgaag acatctatgg caacgtgtgg
    36901 gccggtacgg atggtggcgg cgctgcacgc tacgatcgta acaaaggttt tttcagagta
    36961 ctgacgatga aagacggatt gagcagcgat attgtttttg caatacactg tgaccgggac
    37021 agcgtatatt ggtttgcgac gcaaagcggc attacgcgct acgatccacg cgccacagat
    37081 ttgccgtttg cttcgctcac cgtacgccaa ggtttgccgg ccgatgcggc atttgaaatc
    37141 ctcgaagata atcacggtta catctggctt ggcggtgcag aaggaatttt tcgcatcagt
    37201 aaaaaggatt ttgaagatgt cgcgcgcggt aaaaccaatc aattgacccc gcttcttttt
    37261 ggcagaccgg acggtatgaa gaccagccaa acaacccctt cgggacgtgc gcacaaaaca
    37321 tccgacggaa agctgtggtt tcccacccct aaaggcgccg tggtcatcga tccggataaa
    37381 ataaaaacca accctgtacc gccacccgtg gtgatcgaac gatgcatagc cgatgggcaa
    37441 tcatggccga tacaaaacga tgtggtattg ccgcccggta ttttgcgaat tgagtttcaa
    37501 tacgccgctt tgagttttct cgctccgcag cgcgtatttc acaaatttaa actcgaagga
    37561 tttgacaccg attggatgga tgccggtaca cgacgcacgg cattctacac aggacttcat
    37621 cccggcgaat acacttttaa agtcatcgcc tgcaacaacg acggcatctg ggcatcggag
    37681 ccgacttcgt tttcttttac gattcgtccg catttttacg aaacacggtg gtttttgctt
    37741 ctcattttcg gcggtatgat cgctgcggct ttatcattat accgttggcg tgttcgccac
    37801 ttgatgcacc gtgaaaagga actagccgca ttggtcaaac tgcgcacgca taatctggaa
    37861 gaagaaaaaa aacgcaccga ggacgcgcgc atcgaagccg aacgtcaacg cgaagaagcg
    37921 ttgcgcgcca atgagcttaa aacacaattt ctcggtatgg catcccatga cttaaaaaat
    37981 ccgttagcgg ctatcataag ttataccgat ctgatcatcg aagcgtatga cgataaagct
    38041 cagtccatgc gcgatctggg ttatattcgc aaggcggccc atcgcatgct tcatcttatt
    38101 aacggtcttc tcaatacggt tagcctggaa agcggaaaag tcgtcctgca aaaagaaaaa
    38161 ttagatgtat ccgatttggc ggaacaggtt ctttttagta atcaacaaat gctggaacga
    38221 aaacgtcaac atgtgcaggt acaaactgta cccggactct atgttaacgc cgaccgtgac
    38281 cgtctctacg aagcgctgga taacctgatc agtaacgcga tcaaatattc agattacgat
    38341 aaaacaatac gcgtagattt gggcatgaat gaacttaaaa tcataattcg aatcacggat
    38401 gaaggtcccg gaatgactac cgacgatatg aaaaaacttt tcggtaaatt tcaacgtctt
    38461 tcagccaaac ctaccggtgg agagacatct acaggcttgg gtttgtctat cgtcaaacaa
    38521 atcgtcgaaa tgcatgaagg ccaggtttgg gccgaaagtg aatccggtaa aggcagtacc
    38581 tttgtcgtag agttaccaag attcacttca tagcaaacga atcattttat cgtttataca
    38641 aggaaagacc catcatacaa ccaactccga tccgaccgga ttgaattttc aataaaacgg
    38701 cggatcgcca ctcactgtag cggccgagtt tgaatttgaa ttcaagttgg caaaggcggc
    38761 atatccattt tgaattacat ataaaaaaat catagtgtat ttatgcaatc atgtcatcct
    38821 cttagaagcc gtgttcaaat aaccctgatt tggttacatt gccttttcgt caccggttcc
    38881 ttatacgcgc tggatccgga tcggcgcgtg acacaataca tgcatacgat gtggacgacc
    38941 gatcaggggc tgccacaaaa taatattacc tctatgctcc aaacgcggga cggctatatc
    39001 tggtgtggca cgttggatgg gcttgtacga tttgacggtg tacgctttga agtttttgat
    39061 aagcgtaata cgccggccat tcgcaataat gcaatcatca cgatgttcga agatcgctcc
    39121 ggtggtttgt gggtaggtac cataggcgga ggcctagtgc attataagaa cggaaaattt
    39181 atttcttacg gattaacgga gaagtttccc agtgcgtata tcttgcatat ggccgaagac
    39241 gaacaaggac gcttgtggtt cgctacgcgc gacggattgg tcactcgcgg cgatacgtcg
    39301 tggatcacct atacggcaaa agaaggttta acgcatactt ccgtacgttc gttgtttttt
    39361 gatcgtaaag gacggctttg gatcggctct tcggtcggac tcagtttaat ggaaaacggc
    39421 gtgattcgta aaattttcgc gcacgaactt gccgacaaaa acgtcacttc gatcacttcc
    39481 gacagcacgg atatcatttg gattacggta ctcggcaaag gcgtgtatcg tattaaggga
    39541 gatagcatcg cacacttgca agcgcccaaa agtattcctc ataattttat caatgcgatc
    39601 gctcgtgata aaaacggtaa tcgctggttg gctaccgatg gcggtctcta tcgtgaacat
    39661 cacggacgct ttgaacgatt tatggatgaa tcgaattttc ctctcgacaa agtcatgtgt
    39721 ctgctcgaag atcgcgacgg caatttgtgg tttggcacca acggtagcgg gcttaaccgc
    39781 ctgcgtgatg aaaaatttat cacctataca acccttgaag gtcttaaaga caacagcgcc
    39841 aacgtcgtat tcgaagacag ccgtgcccgc atctgggtcg gtaccgaagc cggtgtttcg
    39901 atcattgacg gcgatacgat ccgtaatctc ggcgaatcca gcggactgac caacaccaaa
    39961 gtgcgatcct ttgcggaaga tacagacgga cgaatatggg tcggcacgcg cgaaggtgtt
    40021 tttgtatgga atgaggtacg tttcgaatca tggaatacgc gcctcaaaat tcctcccttg
    40081 gtcattctct ccatgctgcg cgcgcgcgac ggtacgatgt ggatcggcac gggcggcgct
    40141 ggtgcggtat catacaaacc tcaaaacgta caacaatata cctccaaaca aggtttagcc
    40201 ggcatcgtcg tgatcgctat ggcggaagac tcgctcgggc gaatgtggtt tggcaccaat
    40261 agcggactga gtcgcttcga cggaaaaaac tttgtcacat acaattccaa aaacggtatg
    40321 tcgtctgata tttttttatc cctatatgta gacaaaacca atacgctgtg ggcgggtaca
    40381 tttggtaacg gactcaaccg tttacaaaac gactccatcg ccatatttac gacggccgat
    40441 ggtttattca acgaaaacat gtattccatt ctggaagatg gcgaaggtta tctgtggatg
    40501 agctgcggta agggcgtgtt tcgcgtcgct aaagaagatt ttgaacgata ccgtcagaaa
    40561 ataatatccg ttataccatg caaagtgttt ggccgcagcg acggtatgcg cagcagcgaa
    40621 tgcaatggca ctacacaacc cgccgcatgg atgacccgca gcggatggct tctgtatccg
    40681 accggtaaag gtcttgct
//
LOCUS       DAOQGF010000014        40235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_414_length_40235_cov_6.714410, whole genome shotgun sequence.
ACCESSION   DAOQGF010000014 DAOQGF010000000
VERSION     DAOQGF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 40235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40235
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_414_length_40235_cov_6.714410"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PK937_03725"
     CDS             complement(<1..569)
                     /locus_tag="PK937_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNH31710.1"
                     /translation="MLTQLLRGTSYPFTAFGFIVHHHLLRWVLAPFLINVLLFGAFFV
                     WTIIHIGDVIHWMETGFLGEMNIVLFWIVAAIMLTIYVLIMAYIWSLIGGLVAAPFNQ
                     YLSVQSEHIEGLVSDHTTSWWHDLYRALVAELKKISFLLFLITPLVLLPLMLLHLIPG
                     FGSVLYAILMFAYVSFATAFSFLDYVLENR"
     gene            complement(580..1800)
                     /locus_tag="PK937_03730"
     CDS             complement(580..1800)
                     /locus_tag="PK937_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH31711.1"
                     /translation="MAIPKSFSIFAGIGVFLSAMVVSLWICEGILWLMGYNPLLYYAG
                     MPNHQYHHKTFEFNVAYEQNEFGFRTPNVSFEKPRNTYRIVILGDSYTYGLGVEDAEV
                     YARQLEVRFNTFFKSTSSPRRVQVINLGISNRAIGDYLAFYQSVGIRYQPDLTIVSFF
                     EGNDVSDEVSEVLPTGEVLDETSVYAFIRNSLQGISPRLFKLIKITKKQWKHKALTEF
                     RAVLPWPEDKLVQYLMASGIDSAYAVKRIKEIPDAMREDALANRIYRPAFARWCAGQQ
                     ANIVLPDTLTMRRNEKFTAETFGRLVDLARHNHSDIMLAAIPSPFAVVQKEKLVWAVS
                     SPPETVQEQIRNLVIRMCKENQTAYIDLKPALKAADQAGETTHFAYDLHWTARGHRLV
                     ADTLFERIKDRVIN"
     gene            complement(1800..3170)
                     /locus_tag="PK937_03735"
     CDS             complement(1800..3170)
                     /locus_tag="PK937_03735"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNH31712.1"
                     /translation="MEKVISHIESNKDRYVEELKDFLKIPSISTNPENKKDVQHCAEW
                     VAQHMTHIGLNNVKVCPTAGHPVVYGEWLGAPGKPTVLLYGHYDVQPVDPLNLWTSGP
                     FEPTIRDGYIYARGSADDKGQVFLNLKSIEAHLKINGSLPVNVKLMIEGEEEIGSEHL
                     EPFMKENKEMLKADVVLISDTSMFDDGIPSIAYGLRGLCYMQVELTGPNRDLHSGTYG
                     GAVGNPINELANMIAKLKDKNGKITIPGFYDKVKPLTKAQRDAYKKLPFNKKGYLKDL
                     GIKETFGEKGYSILEQVSGRPTLDCNGIWGGFQGEGAKTVLPSKAGAKISMRLVPGQK
                     PAEIAKLFEKYIKKIAPKTMQVKVIALHGGDPAMTDITTKEMTAASTAMQKVFKKKPL
                     FTREGGSIPVVAGFERLLGLKTIMLGFGLDTDAIHSPNERFKLDNFHKGILTTAYFYD
                     EMAKMK"
     gene            complement(3245..4219)
                     /locus_tag="PK937_03740"
     CDS             complement(3245..4219)
                     /locus_tag="PK937_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNH31713.1"
                     /translation="MHLFLLIFLAVTMQTFAQEAKLRYAIAIHGGAGVISKDMPDSVK
                     NKYIAGLTEALQKGVAMLEAGRNGLDVVETVVRALEDNPLFNAGRGSVFTADGTHEMD
                     ASIMNGKSLACGAVAGVKTIKNPISLARLVMEKTSHVFLSGEGAEVFAREMGVDTAGA
                     DYFYDAYRYEQWKKAKQKHQIRLDHSADEKKGTVGCVVLDNEGNLYAATSTGGMTNKR
                     KGRIGDSPIIGAGTYASNASCAVSCTGTGEQYIRHTIARDLAARVEYKNETLSQSARY
                     LIHEVLEKGDGGLIAVDKKGHIVLEFNTPGMFRGAADASGRFEVKIWE"
     gene            4367..7627
                     /locus_tag="PK937_03745"
     CDS             4367..7627
                     /locus_tag="PK937_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH31714.1"
                     /translation="MNRKSLYLILFCWIMTAMPMHAQQPFLRYYNLSSGFPHAQVWDI
                     VQDANGILWFATTGGLVRYDGIEYTVYTKDEGLPGNTIRNLSLDSSGIIWLGTDEGLV
                     RFDGKNFTTITISDNGESLAVWQLIKDRTGRTWITTDRGIYLQDTAPYHHIKISHLPE
                     SASFRTAFVDSYGHIWFAGSRGIAWKYFNGTTYTDSFFVIPRSPSAFPSYVSEMAQDA
                     RGRLLFATDNGLGVLDINAMLDQKSLDVRAFTRLYDSRNGLRSSKIKSVCAANDASVW
                     IGTELGFAKFTNGNFENIFADENVSSNSCVSIFSDREGIIWVGTDGGGVIKLPFHNIR
                     NFTVKDGLAFNVVNAITGDGKGTIFIGSDRGVNRWDGKKMHLITPNNPLANTIWSLHV
                     DRKGVLWIGSEQHIYTYEQGQLTDRTDIFTKTGGPIVDIEEDLTGQLWFGTLSGLIRW
                     DGLSATVINKDSGLAGNAVWSVFADSQNTIWAGIDGGLSRIEQLPDHTNHITNWTDRD
                     GLIDNTVNVVRRDPSGIVWVGTDAGISRFDGSHFINYKPKSLGFGDNVVPVLAWDFMH
                     NELWVGCKGFGRVRVDAGMQVTKMLNRNRGLPIDESTTNNAFFIDEKHQIWIGTFGGV
                     TCYDEKSAPMNMPAPHVLIQRIRSDDSVFVLPDFTPHLNDNSWTIETNTVMFEFVAPS
                     FLDEKDNWYQYRLEGFDQTWSEWTRKSEVRYTNLGPGTYTLHVRARNAEYVLSSDEAR
                     ATLHIPRPFWMNPVIMLIFTGIALYGAYGTYRIRVAFKLRSMRDRTQRLESMVNQRTS
                     EIERQKNELEGLLDQLKRTQSQLVQSEKMSALGQLVAGVAHEINNPTSVLTGNVAYVG
                     DYIKVLRQLIAKYESLLPASREIASYKEALDYEFVISDLDNLLSAIQHASDRIRLIVK
                     DLRNFSRLDEADLNQVDIHECIETTLKLFYNQYRHILRIEGQYEATKAVYCYANQINQ
                     VLLNLFINAAQAIEQRFRGKKTIEGVIRIHTANTENGIQVKIRDNGAGIPPSIRSKIF
                     DPFFTTKPVGQGTGLGLSISYSIIEKHNGSISVDSREGEFTEMTFSLPLQPKTLPS"
     gene            7651..8112
                     /locus_tag="PK937_03750"
     CDS             7651..8112
                     /locus_tag="PK937_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNH31715.1"
                     /translation="MNVVDSELFKKLGEDMKAAMKSGEKEKLDIIRTLKAGLQKVLID
                     KGQNFKAEDEMNFLLAESKRRKEAIELYEKGGRQDLADKEKTELALISEFLPKQLNDD
                     ELAAIIDEAIRTTGITSAKDMGKLMGALMPKVKGKADGKKVQDMVKSRLAG"
     gene            complement(8200..10008)
                     /locus_tag="PK937_03755"
     CDS             complement(8200..10008)
                     /locus_tag="PK937_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNH31716.1"
                     /translation="MIERFKINLIFSIALKWLCIVLFVARIHAQNAPKKINEFSAFDA
                     TVDHMTYNDDGSKLVTACKTRHEVAVWQGVSGDPLMFSRLYKIPEANNNLGSEAFLFY
                     KFMTVSPDKNWLAFVLPDSSVQILDMHLTEFSRTRFSAHVQILTFSWDLKTVFIRTDE
                     AAYLSDFNNGEIQNLRIISKDPDCYAELFSGDNKLLIGDRYMWTLDGKFLGAFDRLGG
                     FDLVMSPNKKYFTGGAWLWNMDKMIVADMENKKLLHPAYSHDGCYLAGVENYSFRIMD
                     SQYRIIYEYTMPRKKQPGKKNEYVTYTATAFSPDSKYLTLGMSDGTIQIWEMPTSLIM
                     SHNGGIVSLAVCDDGTVISSGGEGNLRWWSKGNFIKQITQVYATDIAVSDKQHIATAG
                     RNGAIHIYDKQGQELTRWGAHTGEVTNVEYMPATDEWISAGSDGAVFAWTSGGKKTRT
                     ILPESGDPILSLSVENTTGQIIVSTASGSVYGGGSHEPMKKILTQPVWIPAVQWSRED
                     SYYFSTRTNEVGRAFLGGQADQTKLKGAENIHVFVKAGDRIFAGDTDGNVWRMNGLER
                     EIVYTGSTAVTALSASPDGRVVYAGFMDGLIFKLQK"
     gene            complement(10001..11068)
                     /locus_tag="PK937_03760"
     CDS             complement(10001..11068)
                     /locus_tag="PK937_03760"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNH31717.1"
                     /translation="MLRWLLRSYAVFCINLFYVAAQDSWPSPYFDPVSYDYGYKDSLG
                     RLVLPVQYDDARMFVHGIAAVKQNGLWFYIRKDGSTVFNGKYEVAGSFSEGYAVVWDK
                     AEGHRLIDTTGRVQLRQANMKDVIRGRLVTSNPRQLPLTGLMGLQTLDGKTIVEPFFP
                     DDESYVIRNDNGLFSTEGMQPPDPQRIPFQLRGLWGFVDAQGRIVMMPRYREARPFQE
                     GLAAVRVDSLWGYIDTAGRFAIAPQFDDAKMFSEGLAGVRMGNLWGFIDKSGKVIIPF
                     RFQVVKPFSEGLAVARVPNMKSRNNNTVLHGYIDKTGRWVLGPRWIDAYSFSGGRAVV
                     VETFSSGIPYEIDKKGREYRD"
     gene            complement(11181..11630)
                     /gene="ruvX"
                     /locus_tag="PK937_03765"
     CDS             complement(11181..11630)
                     /gene="ruvX"
                     /locus_tag="PK937_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNH31718.1"
                     /translation="MAFFMEKGRILAIDYGERRVGLAITDPMQIIASGLTTLAVENDR
                     HAANQIVQIISKEDVTEIIIGLPIAPSGEATEKTRTVNQFISILKEKTALPIIPWEER
                     FSTVSAQRLLIEAEEKKKRRTKEKIDTLAALVILQHYLDYLRLNPKK"
     gene            complement(11673..11990)
                     /locus_tag="PK937_03770"
     CDS             complement(11673..11990)
                     /locus_tag="PK937_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNH31719.1"
                     /translation="MKTMTKKDVSKRVADKLGQKIHEVEHVVDIVFDSIREILSEADP
                     DVRIEVRDFGVFEVKTTKAKPKARNPKTGEIIFVPPRRKTHFKPGKLLKEVLKQPLKG
                     EQE"
     gene            complement(12091..12870)
                     /locus_tag="PK937_03775"
     CDS             complement(12091..12870)
                     /locus_tag="PK937_03775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH31720.1"
                     /translation="MIRPSDFANWQEYYFTYQNILAKQYLIPMLEQWGVSIDGKKILE
                     IGCGSGGVIEAFAERASKAVGVELNPFDYTKLNTPKVQYVTADIYDPSHRERYADKYD
                     IILFRDVIEHLPKKKEAFEICDTLLSENGVIFITFPPYHGPYGAHQQVFSKTFLGRLP
                     YTHLLPNSLYIKYVKAVEKGNDAALQVAHELIASQTTIGSLKKSIRASAFKIDRYDYY
                     LVRPSYEIRYGKKPRKINLMKYLPFIREFFVLGMYMIMRRK"
     gene            complement(12867..14489)
                     /locus_tag="PK937_03780"
     CDS             complement(12867..14489)
                     /locus_tag="PK937_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31721.1"
                     /translation="MKNKESKIKNTSPLKSSGLADWNQTKTWLILSLSTVVLTLIYYM
                     VNSPRPYDDDNIGRYFMAQAAFVKPGYFLDIWGRPMAILLFSIPAQFGYWYVASGVAL
                     LTVMTCFFTYKAAQESGRPYAWMVVLFVFFQPLVLTTSFSLCAEPLAAFFVAIALYFY
                     YRNKLWQAALLFSMVPLARSETVLILPLIAIPWLRERRWIPVLLLGTGLAVYQIAGMI
                     STGDPLYLLTQSKAFGHGLYQNGPFEHYFQRFIFITGPVIFVFLLFQLASDIRLRKLS
                     LLNTSVVLMFAVHVYFYWKGNVASVGFLRHFVAVGPMIALLALDGFNRWIGFEDKQKT
                     NSVVWDAFILAALSVLILAYYSFELVGQYFLSDKAEHLKFGTALVLFLLFILRAVLKL
                     DGIFFKKIMITGTVILTVAYCFVKEKPLTFAPEHQTVKNCYDYYKENIRGKTPKVMIA
                     HTWWFFFDNFNNYSKTLEEGGYENMRKEKLADLPVGSFVIWDSHYSHRLSSNVQLRED
                     LAGDKNFQLKQQFLSSDQKFVIYIFEKVKQPS"
     gene            complement(14507..15175)
                     /locus_tag="PK937_03785"
     CDS             complement(14507..15175)
                     /locus_tag="PK937_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31722.1"
                     /translation="MRLRFLLCTVLMLSYSFDVLAQEQRPSGPSKKKAIILSLLAPGT
                     GELYLSDWKFEQWGSGKYFAATEVLFWATNFYTRSYSGWLKEDARALAARKAGVDIRS
                     TKPAKYYVNIGKYRDIYTYNETQRRATGTEFLYDETDANYWQWQKDSDRRKYDKMRAD
                     TKRFRQVSTYMYFGLFVNHVMSIVHVTRQYKRMTTAQSTTGWRFYIEPSSTGTACIQL
                     VKTF"
     gene            complement(15172..16602)
                     /locus_tag="PK937_03790"
     CDS             complement(15172..16602)
                     /locus_tag="PK937_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH31723.1"
                     /translation="MLRRIRAKIQNKLIVTFAAFCLTIFSASGIIHYWYTESSLDEEL
                     GKKLLAVAQAAKLQIHTEMVSKLESGDENSRTYRTFKQKLTDLRDATDVERIYIIDPN
                     GRSLMDTEEGVRIGYAYPFLKFNQYEFERAAAGLPSHSILFETYDGKLYKTAFVALTV
                     NGQTHGVIAVDGSATFLYLMKRIEWHLITVGIIGVVVSVAIGFYFSRSISTPIKQIMV
                     EAEHIGSGKLDRKIAIQTGDEVGFLGRTMDRMRENILKRDQYMKTMLAGVAHELRNPL
                     GGMELYAHLLNKEIPDGDNRKKAILKVLRELDVMKQIVNDFLDYARPKEPQPMVCDLS
                     KLIDEIRINLSALTTEKSVQWEVELNEKQVYADPGHLRQILINLIENAMQSGNVLPRV
                     RIVSQRVNAMIEITVEDNGHGIEDNIKEKIFDPFFTTKEKGSGLGLAIVKKFVDENDG
                     DITITSSGTNGTMVRLMFPTKEGVSA"
     gene            complement(16681..17568)
                     /gene="xerD"
                     /locus_tag="PK937_03795"
     CDS             complement(16681..17568)
                     /gene="xerD"
                     /locus_tag="PK937_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNH31724.1"
                     /translation="MKHHLQDFIDYLSVEQNVSPNTLASYRNDLERYLKFVMGSRERK
                     KIEDVTMQDVRELVQTLDGLGLTAKSIARNLSAIRTFHKFLLQEEILENDPAELIDLP
                     KIEKTLPSVLEVPEIETLLEQPDTSSRLGIRDRAMIELLYACGMRISELLTIKQLDIY
                     SDYVRVFGKGSKERIVPMGDTAGYWVTYYREKVRPHLAASGSADEVLFLNAQGKRMSR
                     MGFWKILKKYVTMAGIQKEVTPHTFRHSFATHLLEGGADLRVVQELLGHSDISTTQIY
                     THIDREYLKEVHKTFHPRG"
     gene            complement(17871..18965)
                     /gene="add"
                     /locus_tag="PK937_03800"
     CDS             complement(17871..18965)
                     /gene="add"
                     /locus_tag="PK937_03800"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNH31725.1"
                     /translation="MAKKELNRIEVTEELIHRLPKTELHCHLDGSNRVETLIELAKEQ
                     NVQLPFEDPVKLKSYLEVGDKCENLVDYLKGFAITLSVMQEAYAIERTAFELAEDAAK
                     ENVRYLEVRFSPILHINRGLRLTEIVDAVIRGLERAESKYKIKTGIIICGMRNISPET
                     SLRLAELTVAYKGKKVMAFDLAGQEDSYPAKDHRAAFELILKNNVNTTAHAGEAYGPD
                     SIKQAIHDCGAHRIGHGTRLIEDGDLLNYVNDHRIPLEICLTSNVQTKAVKDFSSHPL
                     RTYYDFGLRVTVNTDNRLISGTTVTKEIYRAAKTFDLSFDDVCNIIINGFKSTFLPYR
                     ERVVLLNDALAELQSFEFESSYIKDKAQAN"
     gene            complement(19011..20228)
                     /gene="sucC"
                     /locus_tag="PK937_03805"
     CDS             complement(19011..20228)
                     /gene="sucC"
                     /locus_tag="PK937_03805"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNH31726.1"
                     /translation="MNIHEYQGKAILKKYGVPIQEGIPAATVTEAIDAAKTLKAQGIG
                     AWVVKAQIHAGGRGKGTIYKAKERNEVVQHGGVKFTPDINKVAEYAEKMLGNVLVTHQ
                     TGDQGKLIKKVLVAEGLDIAKELYVGLVLDRARSQYVMMASTEGGVEIEKVAEEHPEK
                     IIKEYVDPTVGFRAFQAQRLGFALGLEGDIHKNFVKFAMALYDAFIGTDSSLFEINPL
                     VITKDNRVVALDAKINFDDNALYRHPDLAALRDIDEEDPLEVEASKYNLNYVKLDGNV
                     GCMVNGAGLAMATMDIIKLAGANPANFLDVGGGANPQTVENGFKIILSDPHVKAIFIN
                     IFGGIVRCDRIANGVVQAAKNVNIRVPLIVRLKGTNAEEAKEILRKSGIPMLVANELN
                     DAADMILQAVKKN"
     gene            20585..21742
                     /locus_tag="PK937_03810"
     CDS             20585..21742
                     /locus_tag="PK937_03810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNH31727.1"
                     /translation="MKQKYDVVVIGAGCIGASVASHLGELGCANVLVLEKEKLIGTGS
                     TAKCAGGVRAQFSTPINIYMSHYSIREFETLEKEYGIQFNQCGYLFVVRNEEQKERYL
                     KNMEIQQSYGVDVRFISKDEIAVLAPNYGLDNVLGGTFGAKDGLIDPSMTVDAYFKRA
                     RKHNIDFETETPCTGFKTSGNRITHVITPKGEVACDMVVNAAGPFSKELGKMLGFEIP
                     IEPVRRMITTTGELPFVTNKFPMVVDVTTGMYMHKEGNGLLIGLANKNEKPGYDENID
                     TEFLDEMLTTALEVMPYLENAEIKTQYPGTWGGLYEETPDHHSILSYVPQFSNFIVAG
                     GFSGHGLMHAPAAGRAIAELVTKGTCDTFDLKPLRFTRFAEGDLTKERNVI"
     gene            21802..22824
                     /locus_tag="PK937_03815"
     CDS             21802..22824
                     /locus_tag="PK937_03815"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNH31728.1"
                     /translation="MATKKAKKKNMFDAIKPVIAKAKTFVLTTHVNPDGDGLGSEAAM
                     YLFLKSLKKSPRIINTSPIPKAFQYLNYGNIIETYEPAKHDKVIQKADVVMVLDISVS
                     KRLDRMQEAVVQAPGATICIDHHLDNDGFADHLCIDEKAPATAELVYNFIRYFKGKLN
                     FKMAQALYTSLLTDTGGFRFNSTRPETHHMAAELLAIGVKPNDIYSQIFEQGRMSSLK
                     LLGRALDRMKSECAEGMHWTYLTKKDFQETGTTKNDTEGFVNYALSLENSMFGVFAYE
                     TDEGFIKFSLRSKGHVDTQAFSKRYGGGGHKNASGISLKEPFDATLKKMIPEMVKYYN
                     ETYGKK"
     gene            22827..24329
                     /locus_tag="PK937_03820"
     CDS             22827..24329
                     /locus_tag="PK937_03820"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNH31729.1"
                     /translation="MHVSAKYSKLKHWKSELTCVFIAKNEPVNFWKNELEDIYEDTIP
                     KLLKSKEFGAEEEKVFLLLGNDKTSSKRIALIGLGEYKDLEPETLRRTAAKAVRTALA
                     MEYSKITLAFPAHLRQKNWTDDRIAQALTEGACLAHYKYDKYHTDKKPQDRHRITELT
                     LIADHEPTAALQQGVRAGEIISNAVNFTRDLGNAPGNELPPRELADRATAMCRTHKIK
                     CTVFNETKIKSLKMGGILGVTAGSAEPPRLVTMEYKPAKPKNTKPIVLVGKGLTFDSG
                     GISIKPADQMDRMKFDMCGGAAVIGIMQAIAELKLPLYVVGIVPSSENLTGSRAYKPG
                     DVLTMYSGKTVEIINTDAEGRLILADALAYAQRYKPEVIIDYATLTGHCVIALGMHGA
                     GLFATDSNIKQKLMHASDASGEKIWELPLWKEFDKQIKSHIADIKNTGGRPGGASTAA
                     AFLKHFVGNTPWAHLDIAGTANSEEELPYTDRISATGFGVRLTVEMLRHW"
     gene            24332..25867
                     /locus_tag="PK937_03825"
     CDS             24332..25867
                     /locus_tag="PK937_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNH31730.1"
                     /translation="MSDIVLTDNDLALLAQIGKSPEQLSAEIEFFKTGFPFVELDRPC
                     TIGDGITVLSDSTHDALIKKADDAAAAGRVKKFVPASGAATRMFKSLMAVHYDRMLQQ
                     NTSHPDTRFLKTFFDRLTQFAFYDELDGVLAKDGLSLETLRQTRSYSDVLSYLMHDVG
                     LDYAHRPKALIPFHRYPEGARTALEEHLTEALAYATDTDQNAYVHFTISSEYRQLIDS
                     HIQTVRSRYETSGRRIQIEISEQKPSTDTLAVDLHNRPVRDQDGRLHLRPAGHGALIE
                     NLNDLAGDIVFIKNIDNVVPDRLKEPTFRYKKILCGHLIEMQLEIFGYLKKLKNNPPN
                     SDTVTEAESFAISRLGMQASSTMDIRERRDNLMQFLNRPIRVCGMVRNQGEPGGGPFW
                     VREPNGNRSLQIVETAQIDLQWPKQKLILDDSTHFNPVDIVCGVRDFEGKPFDLHRFI
                     DPHTGFISKKSKDGREIKAMELPGLWNGAMAKWITIFAEVPLITFNPVKTINDLLRPE
                     HQP"
     gene            26141..27688
                     /locus_tag="PK937_03830"
     CDS             26141..27688
                     /locus_tag="PK937_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNH31731.1"
                     /translation="MKKEIIINATDAETRIAIVEDERLVEIFVERPEKNRMVGDIYKG
                     RVAKVFPGMKAAFVNIGMKQDAFLHFSDIGDNTHEYAVINDADDADDNEAPVKPFNHP
                     RIHNHDNHGRFLRVGQEILVQVIKEPIYNKGARVTSEISIPGRFTVLIPNDPNEMIGI
                     SRKINTVPERKRLRRLAKEVKPRDFGLIVRTNAEERDEEVLRNDIRNCLDTWHDIDTK
                     AQKMSAPNLVYKDMEMVSSVIRDLFSNDVSRVLIDDKKLYKSISNYISTTSPQLGNRI
                     ELYRSNKPIFDLYNIEQDLQQSIEKKVPLKNGGYIIIEHTEALISVDVNSGKYIGRGS
                     YEENSMAVNMVAARETCRQLRLRDIGGLIVIDFIDLHDERNKKKLFEEMKKELRKDRA
                     KFSILPMDEYGLIEMTRERIRPSIMFSLSEPCPACSGTGRVGNRTTLFDKIERWARRY
                     KEKNSFFSKLEFVVNSDILEQVKPKIKNMRWRYLIFSRVTGEQEIRMDEFRVLQKNED
                     ITEMYGS"
     gene            27722..28489
                     /locus_tag="PK937_03835"
     CDS             27722..28489
                     /locus_tag="PK937_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH31732.1"
                     /translation="MFLHATTIYGPLTTRRLGTSLGINLLPVGRKVCNFECVYCECGW
                     TDTSVKDAVPTLEAFTEALESKLSALRQQNQNIDHITFAGNGEPTLHPNFSAIIDTTR
                     EKREKYFPNARIAVLTNATRVHKTEIFDALKKTDDPILKLDAGTETMFQRIDIPDKGI
                     SLDGITETLKKFNGNLIIQTLFLRGEHQNTVIDNTTPEEVHAWLRRIEAIKPRKVMVY
                     PIDRDTPVKTLEKISATELEKIAAQVRTLGIPAEVFG"
     gene            28510..29355
                     /gene="folE2"
                     /locus_tag="PK937_03840"
     CDS             28510..29355
                     /gene="folE2"
                     /locus_tag="PK937_03840"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNH31733.1"
                     /translation="MSAIEDTQGRDDFRKMPIQRVGIKNLKYPLTFINAAGRRTPTVA
                     MWGLAVDLPHDKKGTHMSRFGEILNAWQDGLSIESMPYFMNMMRERLHADRAFARVTF
                     AYFIEKEAPVTLTKSFMDYTVAIDASSIHDKMTLSLEVQVPVTTLCPCSKSISEYGAH
                     NQRSLVTVKAECETDNFLSIEELVYSVETCASSELYVVLKREDEKYVTERAYDHPAFV
                     EDLVRDIAIKLSQDARIVRYRVDVTNLESIHNHDAYAVIESQSVSSVLSDNGAKHHAS
                     PEQQS"
     gene            29545..30312
                     /locus_tag="PK937_03845"
                     /pseudo
     CDS             29545..30312
                     /locus_tag="PK937_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            30326..31096
                     /locus_tag="PK937_03850"
     CDS             30326..31096
                     /locus_tag="PK937_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH31734.1"
                     /translation="MDYTTIRVEHHAREAHVTLNRPPVNVINLSMIDELNVALDKITS
                     NPDVTTIVFRGAGDKAFSTGVDIKDHTLDKINSMLKSFHHIFRTLANSDKITVASVHG
                     YCLGGGMELATFCDFVIASDSTIFGQPEIKVGCYPPVAAAWLPQKIGLRRAMEICMLG
                     ENIKAHEALTLGLINKVVPPDELRSATDTLLQNLNALSGTVLKQTKRALLAGADKPFT
                     TALDTAENIYLQELVKTHDIEEGITAFMQKRKPEWKHR"
     gene            31202..32914
                     /gene="sucB"
                     /locus_tag="PK937_03855"
     CDS             31202..32914
                     /gene="sucB"
                     /locus_tag="PK937_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HNH31735.1"
                     /translation="MAKVDMVMPQMGESIAEGKILKWHKKVGDKISKDETVLEISTDK
                     VDSEIPSPASGIIAELIVPEGTTVAVGALIARIETDANASVASPAPTPSPKVESKTAP
                     VAQNTPASKPASGGASVDMVMPQMGESIAEGKILKWHKKAGDKVAKDETVLEISTDKV
                     DSEIPSPASGVITEIIVPEGTTVAVGTLIARIGAEGATVAASTTAAPTPKTEEKMHFS
                     SSIEDFQKQNQIAATAATPAATVNAGGGMVKGESNRFYSPLVRSIAKAEGLSMQELDS
                     IAGTGLNHRVSKNDVLAYLAKRPKGGVRPAVTTTTSASTAAAPAKQVSDVKYGYNKER
                     SELIEMNNIRRITAEHMRRSIDTSAHVYSMTEIDMSRVVRFRDKYKSAFEKREGFKLT
                     FTPFLIDAMVRAIKDFPMINISVDGNTIVKKNYTNVGMAVSIENNTALIVPVIKDADA
                     MNLVGVCRATNDLALRARSKKLRPEDIQDGTITLTNMGVFGTTVGFPIINQPQVAIMG
                     VGTIKKRPVVITTDDGEDMIVIRPIMYASLSYDHRVVDGALGGQYLQRVAYHLENFDE
                     NQTI"
     gene            32979..33611
                     /gene="thiE"
                     /locus_tag="PK937_03860"
     CDS             32979..33611
                     /gene="thiE"
                     /locus_tag="PK937_03860"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNH31736.1"
                     /translation="MNKRLNVSLYFIADVGMIPAGKICSVVEEAIQGGVTFVQLRAKT
                     LSVNAMIALGRTIHQVTYSKNIPLIINDRIEVATAIGAEGIHLGQEDITVGAARKILG
                     DSAIIGISTHTQQEAQTAESAGADYIGVGTVFPTASKKDIVGIIGTTGLKQIRDLVKL
                     PVVAIGGINQNNVKEVLACGVDGIAVISAIGLSVNPRETTRALKDLLTVN"
     gene            complement(33624..34667)
                     /gene="ruvB"
                     /locus_tag="PK937_03865"
     CDS             complement(33624..34667)
                     /gene="ruvB"
                     /locus_tag="PK937_03865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNH31737.1"
                     /translation="MQDITDDGLARPRKVPEDLDLDISLRPSRFDEFVGQQRIINNLK
                     VFIEAARLRGEALDHVLLSGPPGLGKTTLSHIVAHELGVHIKITSGPVLDKAGDLAGI
                     LTNLEERDVLFIDEIHRLNPIVEEYLYSAMEDFQLDIVIDKGPSARTIQLTLPHFTLI
                     GATTRAGLITAPLRSRFGITSRLDYYNDKDLAHIVTRSAEILQVDIDASGANEIASRS
                     RGTPRIANRLLKRARDFAQVRGKKIIDKEIADYALKALEVDEHGLDEMDKRILSNIIE
                     RFNGGPVGIDNIAIAVGEESGTIEEVYEPFLIQKGFLKRTPRGREVTKLAYDHLRIRP
                     RKTSQQAGLDLEE"
     gene            complement(34673..35686)
                     /gene="lpxD"
                     /locus_tag="PK937_03870"
     CDS             complement(34673..35686)
                     /gene="lpxD"
                     /locus_tag="PK937_03870"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNH31738.1"
                     /translation="MASRSYKFSDIAKLLERPFVGSDSEITGFNEPMYALESEVIFLF
                     QKNYVPQSVSGASKFWIVSEKVFAKDLAEQCLKKKTAYILSKDPYEDIIKVLTWWIDQ
                     RALRRGNAFKIKSKLYNGVKTHRSSVIGYDTIIGRGTLISAHAVIGNHVRIGKGCVIY
                     PNVSIYDNTIIGDRVIIHAGSVIGSDGFGFHKTEEGYLKIPHIGRVVVGDDVEIGANC
                     CIDRGTVGETKIGRGCKLDNLIQIGHNVIIGPHTLIAAQAGVAGSTRIGDHVTIAGQA
                     GLVGHINIGDHAVIAAQAGVIGSVDENTTVSGYPAREHRAALRRDATLKKIIEQYDQK
                     DKV"
     gene            complement(35757..36308)
                     /locus_tag="PK937_03875"
     CDS             complement(35757..36308)
                     /locus_tag="PK937_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNH31739.1"
                     /translation="MKKTMIAILMLTLGINTAVKSQGKIGYADSQKILQSLKETETVQ
                     TKLQAEQERIYKQFQYMQDSLQTMQEDYVKNYRDNPLIKDNIKQTFQKGMEELAYLIQ
                     NSQQKYQEEMYKKQQELMAPILDKVKKAIETVRKAKGYDMILDSSPGMILAYDPQLDM
                     TQKTIDELIRMATAAPTTGTGGK"
     gene            complement(36339..37031)
                     /locus_tag="PK937_03880"
     CDS             complement(36339..37031)
                     /locus_tag="PK937_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNH31740.1"
                     /translation="MKYLVWLSLLISFSPVYMGDVCAQGKIGYVDFQYLITQIQSVHD
                     VQTELEKLSRDWTDRLSAMEDSVALIEKDYEALSITLTKSGKNVLEKNIADAKQKILV
                     YREQKFSPVTGELYKKQQELLQPILDKVKRAIDNVRLKEKYDVVFDISTGNPVSIDKR
                     LDLTLLVLEELPSVGLTVAQVEGQGTGTNLKTPGSNPTNRTPSGRSSKSTTNETSKPV
                     KENDTDPAIKDN"
     gene            complement(37047..39428)
                     /gene="bamA"
                     /locus_tag="PK937_03885"
     CDS             complement(37047..39428)
                     /gene="bamA"
                     /locus_tag="PK937_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNH31741.1"
                     /translation="MKLAKIIVITAFLLCLGSNRASALFAQSGAKPKIASIKIEGNIK
                     SDPELIVIATGLSVDQEFNLDDIQKAMENLWQMNVFKDIQVFGEQTENGIAVVFEVKE
                     YPRLENLEINGTDEIEEKDVRSKFGLFNGQTVSPQHIKKATERVRQLYASEGYRNVEI
                     DVKSYNSETDTGKVLLKIEIEENDKVKIRGITFHGNTAFSNGKLEGAFDDTKAKRGFW
                     KWFKSGDFDEKKYNEDLKKLLMFYKKRGFRDIQIMKDSVYYAQNNKDLYIDIWVEEGV
                     KYHIGNISFTGNTLFSGDELTAALGFERGDVYDQEKYDRNMQERVNALYYDRGYIYAQ
                     LVPIERPSNKDTLNLEFVVTEGNQVDIERVEIRDNTKTKEKVIRRETVAYPGEKFSRD
                     ALIRTQRNLMVLNYFETVIPDVQPVSQDKVNVIITVKEKPTDTANLSIGYSAQDGLIG
                     SAGVAFNNFLGNGQVVNLNLQLGGAGYKVFSIGFQEPYLFDTRTSFGASMYYSIDGNR
                     RAQYVGYKSQSYGGSVNFGRRLKWPDDFFTASWSVAYGNSKLKPLSTSQVSDYIAYGH
                     TQSVTLSQVINRDSRDAAEFPKTGSVFTLTSDIGFFSIDTVGYLNSLRVLPKNFFKNT
                     FRAQNYMPLFWNFVFYTDFTMGYVRTFQSTSTVEDIPQFDRFYMGGGALDIGSIQMRG
                     YGGRKVGGQAVTESGISYPIGGTSMVKYSAEIRLPVIPNPTMFILGFAEAGNVFQSLK
                     TTDPFQVKRSIGYGFRLFMPLVGVIGLDVAYGLDRKDKSRNFPKLHFQLGQQF"
     gene            complement(39494..40219)
                     /locus_tag="PK937_03890"
     CDS             complement(39494..40219)
                     /locus_tag="PK937_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNH31742.1"
                     /translation="MPRHIAIIMDGNGRWAKQRGLPRIAGHNEGVNSVKDITEGCGEL
                     GIEALTLYAFSQENWKRPSWEVSALMKLLMRTILNQIDSLNRQNVRVKTIGHIDMLPT
                     GTLNQVEKAIEITKNNTGLVLNLALSYSGRIEILDAVKRLYQDAARKKSLMDSLDESV
                     FAKYLDTFDLPDPDLVIRTSGEFRVSNFLLWQIAYAELYITDIYWPDFRKPQLYKAIL
                     DYQKRERRFGKVSEQIAEEKKEK"
BASE COUNT        11266 a         9588 c         8484 g        10897 t
ORIGIN      
        1 cggttttcca atacataatc caaaaaactg aatgctgtcg cgaaactgac atacgcaaac
       61 atcagtattg catagagcac gcttccgaag ccgggtatca gatggagcaa catgagcggc
      121 agtaaaacca aaggcgttat cagaaacaaa agaaatgaaa tttttttcag ttcggcgacc
      181 aacgcgcggt agaggtcatg ccaccacgaa gtggtatgat cggatacgag accttcaata
      241 tgttccgatt gtacactgag gtactgatta aaaggtgcag cgactaatcc gccgatcaaa
      301 ctccaaatgt aggccatgat cagtacatag atcgtcagca tgatcgctgc gacaatccag
      361 aaaagcacaa tattcatttc accgagaaaa ccggtttcca tccaatgtat gacatcgccg
      421 atatgtatga tcgtccatac gaaaaacgca ccgaaaagca acacattgat caaaaacggt
      481 gctaacaccc atcgcaggag atggtgatga acgataaaac cgaacgccgt aaaaggataa
      541 cttgtgccgc gtaataattg agttagcatg ggttctctat tagttgatga cgcgatcttt
      601 aatgcgttca aataacgtat cggcgaccaa acgatgtccc cttgccgtcc agtgcagatc
      661 ataggcaaaa tgtgtcgttt caccggcttg atcggcagcc ttcaaagcag gtttcaagtc
      721 tatgtaagcc gtttggtttt ctttacacat ccgaatgacc agattcctga tttgctcctg
      781 taccgtctca ggaggtgatg aaacagccca aactaacttc tctttctgaa caaccgcaaa
      841 aggcgacggt atggccgcca gcatgatatc cgaatgattg tgacgcgcca agtcaaccaa
      901 acgcccaaac gtttccgccg taaacttttc gttacgacgc atggtcagcg tatcgggtaa
      961 aacaatattg gcttgttgcc cagcacacca tcgcgcaaag gccggtcggt agatacgatt
     1021 ggccaacgca tcctcccgca ttgcatccgg aatttctttt atccgtttta cagcgtacgc
     1081 actgtctata ccggatgcca tcagatattg tacgagttta tcttctggcc aaggtaacac
     1141 cgctctgaat tcagtgagtg ctttgtgttt ccattgcttc ttggtaattt tgatcagttt
     1201 gaataatcgc ggcgatattc cttgtaatga attccggata aatgcataaa cactcgtttc
     1261 gtctaatacc tctccagtcg gcaatacctc agaaacttca tcggaaacat cattaccttc
     1321 aaagaacgat acgatcgtca aatccggttg atagcggatg ccgacggact gataaaaggc
     1381 taaataatca ccgatcgccc gattggaaat gccaagattg atgacttgta cgcggcgcgg
     1441 cgaactcgtg cttttaaaga aagtattgaa cctgacttca agttgccgcg cataaacttc
     1501 ggcatcctct acgccaagcc cgtaggtata agaatcaccc agaataacta tgcggtacgt
     1561 attgcgcggt ttctcaaagg acacattcgg cgtgcgaaaa ccgaattcat tctgttcgta
     1621 cgcgacgtta aactcaaacg ttttgtggtg atactgatga ttgggcattc cggcataata
     1681 caacaacgga ttataaccca tcaaccaaag aatgccttca caaatccata aggatacgac
     1741 cattgcactg aggaagaccc caatgccggc aaaaatggaa aatgacttgg gaattgccat
     1801 tatttcattt tggccatttc atcataaaaa tacgccgtgg tcagaatgcc tttatgaaaa
     1861 ttatccagct taaagcgttc attgggcgaa tggatcgcgt cggtatccaa accaaatccc
     1921 aacataatcg ttttgagtcc cagcaagcgt tcaaaacccg ccacgacagg gatcgaaccg
     1981 ccttcgcgtg taaaaagtgg tttctttttg aatacttttt gcatcgccgt cgaagcggcg
     2041 gtcatttctt tggtcgtaat atcggtcata gccggatcac cgccgtgcaa cgcgatcact
     2101 ttgacctgca tcgttttcgg cgctatcttt ttgatatatt tttcgaaaag tttggcgatc
     2161 tccgccggtt tctgaccggg aactaaccgc atgctgatct tcgcaccggc tttggagggc
     2221 aatacggttt tggcgccttc accttggaaa ccgccccaga taccgttgca atccagtgtc
     2281 ggacgcccgc tcacctgctc taaaatcgaa tacccttttt caccgaatgt ttctttgatg
     2341 ccgaggtctt tcaaatagcc ttttttatta aagggcagtt ttttataagc atcgcgttgt
     2401 gctttggtga gcggtttgac tttatcgtaa aatcccggaa tcgtgatttt tccgtttttg
     2461 tctttgagtt tggcgatcat gttagccaat tcattgatcg gattaccgac ggcgccaccg
     2521 taagtaccac tgtgcagatc acggttcggc ccggtcagtt cgacttgcat gtagcagagc
     2581 ccgcgcaatc cgtaagcaat cgagggaata ccgtcatcaa acatggacgt atcggagatc
     2641 aatacgacat cagccttgag catttccttg ttttctttca taaacggctc aagatgttcg
     2701 ctgccgatct cttcttcgcc ttcgatcatc agtttgacgt ttaccggcag actaccgttg
     2761 attttcaaat gcgcttcgat gcttttcaga ttgaggaaca cctggccttt gtcatccgcc
     2821 gatccgcggg catagatata gccgtcacga atggtcggtt caaaaggacc gctggtccaa
     2881 agattgagcg gatcgaccgg ctgcacatcg tagtgcccgt acaaaagtac ggtcggtttg
     2941 cccggtgcgc caagccattc gccatacacg acaggatgcc ccgccgtagg gcaaactttt
     3001 acgttattca atccgatatg ggtcatgtgc tgcgctaccc attccgcaca atgctgtaca
     3061 tcttttttgt tttcgggatt ggtactgatg ctgggaattt tcaaaaaatc tttgagttct
     3121 tcgacataac ggtccttgtt ggattcgatg tggctgatga ccttttccat gttgtctccg
     3181 gttcgaaatg atataggtaa ttaaggttaa tttatactga acagaacttt tttttcgttt
     3241 cgtcttattc ccagatttta acttcaaatc ttcccgatgc atcggctgca ccgcgaaaca
     3301 tgcccggtgt attaaactca agtacgatat ggcctttttt atccacagcg atcaatccgc
     3361 catcgccttt ttccaaaact tcatggatca ggtaacgcgc actctgagac aacgtttcat
     3421 ttttatactc gacacgcgca gccagatcac gggctatcgt gtggcgaata tactgttcac
     3481 ccgtaccggt acacgacacg gcgcaagagg cattactggc gtaagtaccg gcgccgatga
     3541 tgggcgaatc cccaatccgg cctttccgtt tattggtcat accgccggtc gaagtggcgg
     3601 catataaatt tccctcgttg tccagcacga cgcaacctac tgtacccttt ttttcatcgg
     3661 ccgaatgatc aagtcgtatc tgatgtttct gtttcgcttt tttccactgc tcgtagcggt
     3721 aggcatcgta gaaataatct gcaccggccg tatccacacc catttcacgg gcaaaaactt
     3781 cagcaccctc accggataaa aacacatgcg atgttttttc catgacgaga cgtgccaacg
     3841 aaatcggatt ctttattgtt ttaacaccgg ctaccgcccc gcatgctaac gattttccgt
     3901 tcatgatcga agcgtccatc tcatgcgtac catccgcagt aaaaaccgaa cctcggcctg
     3961 cattaaacaa cggattgtct tccagagcgc gaacaaccgt ttcgacgaca tccagcccgt
     4021 tccgtccggc ttccaacatc gccacgcctt tttgcaacgc ctccgtcaaa ccggctatgt
     4081 atttgttttt aacagagtcg ggcatatctt tggaaatcac ccccgcaccg ccgtgaatgg
     4141 ctatggcata tcgtagtttg gcctcttgcg caaaggtttg catggtaacg gctaaaaaaa
     4201 ttagaagaaa taggtgcata aaacttacga aaaatatcgt ctgattttac gtgggatgtt
     4261 accattttta cccgtgagat tcaacttttt atacatgttt tgtttttgca tttcgcacgt
     4321 tggacgccta tattttccga catttattca caatatccta cgcggcatga atcgaaaatc
     4381 gctgtacttg attttattct gttggattat gacggctatg ccgatgcatg cgcagcagcc
     4441 ctttcttcgt tattataacc tgagttccgg ttttccacat gcgcaagttt gggatattgt
     4501 gcaagatgcc aatggcatcc tgtggttcgc aacgaccggc ggattagtac gctacgacgg
     4561 catcgagtac acggtatata ccaaagatga aggattaccc ggcaatacga ttcgaaatct
     4621 gagtttggat tcgtccggta tcatctggct tggaacggat gaaggcttag ttcgttttga
     4681 tggtaaaaat tttactacga tcaccatctc cgacaatggc gaatctttag cagtttggca
     4741 actcatcaaa gaccgcacgg gccgcacgtg gataactacc gaccgcggca tttatttgca
     4801 agataccgct ccataccatc acatcaaaat cagtcatctt cctgagtccg cgtcttttcg
     4861 cacggctttt gtagacagct atggtcatat ctggtttgcc ggtagtcggg gtatcgcttg
     4921 gaaatatttt aacggtacaa cctacaccga ttcttttttt gttataccgc gatcaccatc
     4981 cgcatttccg tcctatgttt ctgaaatggc tcaggacgcc cgcgggcgct tactttttgc
     5041 caccgacaac ggactcggtg tattggatat caatgcgatg cttgaccaga agagccttga
     5101 tgtgcgcgct tttacacgat tatatgatag ccgaaacgga cttcgaagca gcaaaataaa
     5161 atccgtatgc gcggccaatg atgcgtccgt gtggatcggc accgagttag gtttcgccaa
     5221 gtttacaaac ggtaattttg aaaatatatt cgccgatgag aatgtatcat ctaatagttg
     5281 tgtttcaatt ttttccgatc gcgagggcat tatttgggtg ggcacagacg gcggcggcgt
     5341 gatcaaatta ccttttcaca atattcgcaa tttcaccgtc aaagacgggt tagcttttaa
     5401 tgtggtcaat gcgatcaccg gcgatgggaa aggaactatc ttcatcggca gcgatcgggg
     5461 cgtcaatcgc tgggatggaa aaaaaatgca cctgatcaca ccaaacaatc cgttggctaa
     5521 tacgatttgg tctctccatg ttgatcgcaa aggtgtttta tggattggct ccgaacagca
     5581 catttacacc tatgaacagg gacaactcac cgatcgaacc gatatattta ctaaaaccgg
     5641 cggccctatc gtagacatcg aagaagacct caccggtcaa ctttggtttg gcacactctc
     5701 gggtttgatc cgatgggacg gcctatccgc aacggtgatc aataaagact ccggactcgc
     5761 cggtaatgcg gtgtggtctg tttttgccga ttctcaaaat accatttggg caggaattga
     5821 cggagggctt tcgcgtattg aacaattgcc cgaccatacg aaccatatca caaactggac
     5881 agatcgcgac ggactgattg acaatacagt caatgtggtt cgccgtgacc cttcaggtat
     5941 cgtatgggta ggtacggatg ccggaatttc acgttttgac ggtagtcatt ttattaatta
     6001 caaacccaag tctctcggtt ttggggataa cgtcgtaccg gtactcgcct gggattttat
     6061 gcataacgaa ttgtgggtgg gctgcaaagg ttttggtcga gtgcgtgtgg atgccgggat
     6121 gcaggttacc aaaatgctca accgcaatcg cggtttaccg atagacgaga gcacgacgaa
     6181 caatgctttt tttattgatg aaaagcatca gatatggatt ggtacattcg gcggtgtcac
     6241 ctgttatgat gaaaagtccg cgcccatgaa tatgcccgca ccgcacgttc tgattcagcg
     6301 gattcgatcg gatgattccg tttttgtttt gccggatttt acgccgcatc taaacgataa
     6361 ctcctggacg atcgaaacca ataccgttat gtttgagttt gtcgcaccgt cgtttcttga
     6421 tgaaaaagac aactggtatc agtatcgcct cgagggattt gaccaaacgt ggtcggaatg
     6481 gacacgtaaa agcgaagtac gttataccaa tttggggccc ggcacttaca cgctccatgt
     6541 tcgtgcacgt aatgccgagt acgtgttgtc atcggacgaa gcgcgcgcta cgcttcacat
     6601 tccgcgacca ttttggatga atcccgtcat catgcttatt tttacaggca tcgctctgta
     6661 tggcgcttac gggacatacc gcatacgcgt cgcattcaaa ctgcgctcga tgcgtgaccg
     6721 tacccagcgt ctcgaatcca tggtcaatca acgcacctcg gagatcgaac ggcaaaaaaa
     6781 cgaactcgaa ggcttattgg atcagctcaa acgaacccaa tcgcaacttg tgcagtcaga
     6841 aaaaatgtcg gcgctcggcc aactcgtcgc cggtgtcgca catgaaatca acaaccccac
     6901 gtccgttctg acgggcaacg tggcttacgt cggagattat atcaaagtac ttcgccagct
     6961 catagccaaa tacgaatcct tgctaccggc atcccgagaa atagcatcgt ataaagaagc
     7021 actggattac gaatttgtta tcagcgattt ggataatctg ttatccgcca tacaacacgc
     7081 ctccgaccga atccgcctta tcgtcaaaga tcttcgtaat ttttcacgcc ttgatgaagc
     7141 cgatctgaat caggtggata tccacgaatg catcgaaacc acactgaaac ttttttataa
     7201 ccaatatcgt cacattctcc gtattgaagg acaatacgaa gcaaccaagg cggtttattg
     7261 ttatgccaat cagatcaacc aagttttgct caatcttttt atcaatgcgg cacaagctat
     7321 cgaacaacga ttccgcggca aaaaaaccat cgaaggtgtc atacgaattc atacagccaa
     7381 tacagaaaac ggtatccagg ttaaaatcag agataatggc gcgggcattc ccccgtcaat
     7441 tcgcagtaag attttcgatc ctttttttac aaccaaaccc gtaggacaag gtacaggtct
     7501 cggtttgtcc atatcctact ccatcatcga aaaacataac ggctccatct ccgtggacag
     7561 ccgcgaaggt gaatttaccg agatgacgtt tagtcttcct ttacaaccta aaactctacc
     7621 atcataattt ataaacatac aaggagtgtt atgaacgttg ttgattccga attatttaaa
     7681 aaactcggcg aagatatgaa agccgccatg aaatccggcg aaaaggaaaa actagatatt
     7741 attcgcacgc tcaaagccgg gctgcagaaa gttttgatcg ataaaggtca aaactttaaa
     7801 gcggaagacg agatgaattt tcttctcgcg gaaagcaaac gccgtaaaga agctatcgaa
     7861 ttatatgaaa aaggcggacg tcaggatctg gccgacaaag aaaaaactga actggccctc
     7921 attagcgagt tccttccgaa acagttgaac gatgatgaat tagccgctat tattgacgag
     7981 gcgattcgta caacaggtat tacctccgcc aaagacatgg gtaaactcat gggtgcgctt
     8041 atgcctaaag tcaaaggtaa agccgacgga aaaaaagttc aggatatggt caaatcccga
     8101 ttagccggtt gaggcattcc tcataaaatc atagtcaata aaaaaacgcc tcaacatgga
     8161 ggcgtttttt attacatcca tcacgtcact tgacgaaaac tatttctgca atttaaatat
     8221 taaaccatcc ataaaaccgg cataaactac acggccatcc ggtgatgcac tcaaggccgt
     8281 caccgccgtt gatcctgtat aaacgatttc tcgttcaagc ccgttcatac gccacacatt
     8341 accgtcggta tctccggcga agatgcgatc ccctgctttg acaaaaacat ggatattttc
     8401 agcgcctttt aattttgttt gatcggcttg acctcctaag aaggcgcgac cgacttcatt
     8461 ggtacgtgta gaaaaataat acgaatcttc tcggctccac tgcaccgcag gaatccacac
     8521 aggttgcgtt aatatcttct tcataggttc gtgcgaaccc ccgccataca cagaaccgga
     8581 agccgtcgaa actatgattt ggccggtagt attttcgaca ctcagcgata ggatcggatc
     8641 tccggactcc ggtaaaatcg tacgcgtttt tttccctcct gatgtccagg caaagacagc
     8701 tccatccgaa ccggcggaaa tccattcatc ggtagccggc atatattcga cgttggtcac
     8761 ttcacccgta tgcgcccccc aacgcgttag ctcttgacct tgtttgtcat agatatgaat
     8821 agccccgttt ctccccgccg tggcgatatg ttgcttatcg ctgacggcaa tatcggtcgc
     8881 atatacttgc gtaatttgtt tgataaaatt acccttactc caccaccgca aatttccctc
     8941 tccgccactg gaaataaccg tgccgtcatc acaaacagca agggatacaa tcccgccatt
     9001 gtggctcatt atcagagacg ttggcatttc ccatatttgt atcgtcccgt ccgacatacc
     9061 tagagttaaa tatttcgaat ccggtgaaaa cgcagttgcc gtgtatgtca cgtattcatt
     9121 ttttttcccg ggttgtttct ttcgcggcat ggtgtattca tagattatgc ggtattggga
     9181 atccatgata cggaaggaat aattttcaac accggcaaga taacagccgt catgactata
     9241 cgcaggatgc agtaactttt tattttccat atcggctacg atcattttat ccatattcca
     9301 aagccaagca ccaccggtga aatatttttt attgggtgac atgaccaagt caaatccacc
     9361 caaacgatca aatgcgccta aaaattttcc gtccagcgtc cacatataac ggtcgccgat
     9421 tagcaactta ttgtctccgg aaaaaagctc ggcataacaa tccggatctt tggaaataat
     9481 gcgcaaattt tgaatttccc cgttattaaa atcgctcaga taggcggctt catccgtccg
     9541 aataaaaaca gtttttaaat cccatgaaaa cgtcagaatt tgcacatgcg cagaaaacct
     9601 ggttcgtgag aattcggtga gatgcatgtc gagtatttgt acactggaat ccggcaacac
     9661 gaacgccaac caatttttat ccgggctaac ggtcataaat ttataaaaaa gaaacgcctc
     9721 cgaccccaaa ttattattgg cttcaggaat tttgtataac cgactaaaca tcaacggatc
     9781 accgctcaca ccttgccaca ccgcaacttc gtgacgtgtt ttgcaagctg tcacaagctt
     9841 acttccatca tcattatagg tcatatgatc cacggtcgcg tcaaaagccg aaaattcatt
     9901 tatttttttg ggtgcgttct gcgcatggat acgcgccaca aataaaacaa tgcaaagcca
     9961 tttcaatgct atggaaaata tcaaatttat tttgaagcgc tcaatcacgg tattctctgc
    10021 cttttttgtc aatttcgtag ggtatgcccg agctgaaagt ttctaccacc acagcacgtc
    10081 ctccagaaaa agagtacgca tctatccatc gcggccccaa cacccagcgt cctgttttat
    10141 caatgtatcc gtgcaacacc gtattgttat ttcgcgactt catattaggt actcgtgcta
    10201 cggctaaacc ttctgaaaaa ggttttacca cttgaaaacg gaacggaata atcaccttac
    10261 cgcttttgtc aataaagccc catagattac ccatgcgcac gccggctaat ccctcgctaa
    10321 acattttggc atcatcaaat tgcggagcta tcgcaaaacg tcccgccgta tcgatataac
    10381 cccataacga atccacacgc accgcagcta aaccttcttg aaacgggcgc gcctcgcgat
    10441 aacgcggcat cataacgatc cgcccttgtg catctacaaa accccataat ccacgcagtt
    10501 gaaaaggaat ccgctgcgga tcgggaggtt gcataccctc cgttgaaaaa agaccattat
    10561 catttcgaat cacatacgac tcatcgtctg gaaaaaaagg ttccacgata gttttaccgt
    10621 ctaacgtctg gagccccatg aggcctgtca acggcaattg gcgcggatta cttgtgacca
    10681 aacggcctcg gataacatct ttcatattgg cctgccggag ctgaacacgc cccgtcgtat
    10741 caatgaggcg atggccttca gctttatccc aaacgaccgc gtaaccttcg ctgaagcttc
    10801 cggctacttc gtatttaccg ttaaacaccg tggagccgtc tttacgaata taaaaccaca
    10861 agccattttg tttgactgct gcgataccgt gcacaaacat acgcgcatca tcgtattgca
    10921 ccggcaaaac cagacgccct aaactatcct tgtatccata gtcgtacgat accggatcaa
    10981 aataaggcga cggccagcta tcctgtgccg ctacgtaaaa aagattaata caaaaaacgg
    11041 catagcttcg taaaagccat cgtaacataa atatctgtgg ttaaataaaa aaagattgcc
    11101 gaaaggcatt agaacagtat ctaaaactaa cacgaacgct taaaaaaacc aaaaaaaaac
    11161 gtaccggctg gcgctgatgt ttattttttc ggattaaggc gcaggtaatc gagataatgc
    11221 tgaagaatca ccaacgccgc taacgtatct attttttctt tggtgcggcg ttttttcttt
    11281 tcttccgcct cgatcaaaag tcgttgcgct gacacggtgg aaaaacgttc ttcccaggga
    11341 ataataggaa gcgctgtttt ttctttaagg atggatatga attgattgac cgtacgcgtt
    11401 ttttccgtag cctcgccgct tggcgctatg ggcagaccaa ttattatttc cgtaacatct
    11461 tcttttgaaa ttatttgaac aatctgattg gcagcatggc ggtcattttc gaccgccagc
    11521 gtcgtaaggc cggatgcaat gatctgcatc ggatctgtga tcgcaagacc gacacgccgt
    11581 tcaccgtaat caatagctaa aatgcgtcct ttttccataa aaaaagccat ggttttttcc
    11641 atggcttatt attcatataa aattatttct ttttattcct gctcgccctt caggggttgt
    11701 ttcaaaacct ctttgagcag cttaccgggt ttaaaatgtg ttttacgacg cggaggaaca
    11761 aatataatct ctccggtttt cggattgcgt gctttgggtt tggctttggt tgttttaact
    11821 tcaaacactc caaaatcgcg cacttcgatc ctgacatccg gatcggcctc ggagagtatc
    11881 tcccggatcg aatcaaatac aatatctaca acgtgttcca cttcgtgaat tttttgacca
    11941 agcttatcag ctacacgttt ggaaacatct tttttggtca tagttttcat tggagaatct
    12001 cctaatgcgc acgcaaagct aaaacaaata aaatcaacta aattcgccca attataaaat
    12061 aaataactaa ttaagtcaag taaaatcagg tcattttctt cgcatgatca tgtacatgcc
    12121 caaaacaaaa aactcacgga tgaatggtaa gtacttcatt aaatttattt tacgtggttt
    12181 ttttccatag cgaatttcat aggatggccg caccaaataa taatcataac gatcaatttt
    12241 gaaagccgat gcgcgaatgg attttttcag ggaaccgatt gtagtttgac ttgcgatcag
    12301 ttcatgtgct acttgcaatg ccgcatcatt gcccttttcc accgctttca cgtacttgat
    12361 atacaatgag ttaggtaata aatgcgtata gggtaatctt cccaaaaaag ttttagagaa
    12421 aacctgctgg tgtgcaccat aaggcccgtg atacggcgga aatgtaataa aaataacgcc
    12481 gttttcactt aacagagtat cacatatctc aaatgcttct ttctttttag gcaaatgttc
    12541 gatcacgtca cgaaatagta taatatcata tttatcggca taccgttcac gatgtgaagg
    12601 atcatagata tcggcggtaa cgtattgtac tttgggagta ttgagtttgg tataatcaaa
    12661 aggatttaac tccacaccga ccgctttcga tgcacgttcg gcaaacgcct cgatcacccc
    12721 accgcttcca cagccgatct ccaaaatttt tttaccgtca atggaaacgc cccattgctc
    12781 cagcatagga atgagatatt gtttggctaa tatgttctga tacgtaaaat aatattcctg
    12841 ccaattggcg aaatcggatg gacgaatcat gacggttgtt ttaccttttc aaaaatgtag
    12901 atgacaaatt tttgatcgga cgataggaat tgctgcttga gttgaaaatt cttatcaccc
    12961 gccagatctt cgcggagctg aacattggaa ctcaaacgat gactatagtg cgaatcccaa
    13021 attacgaagc ttccgacggg taaatccgca agtttctctt ttcgcatgtt ttcataaccg
    13081 ccctcttcca atgtttttga atagttatta aaattatcaa agaaaaacca ccatgtgtgt
    13141 gctatcatga ctttgggtgt cttaccacgg atgttctctt tataatagtc ataacaattt
    13201 tttacggtct gatgctcggg agcaaacgta agcggttttt ctttaacaaa acaataggct
    13261 accgttagta tgaccgtccc ggtgatcatt atttttttaa agaaaatccc gtctaattta
    13321 agtaccgctc ttaaaataaa taacaaaaac aaaacaagcg ctgttccaaa tttaaggtgt
    13381 tcggctttat ccgataaaaa atactggcca accaattcaa acgagtagta cgccaaaatc
    13441 aaaacgctta gtgccgctaa tataaatgca tcccaaacga ctgaatttgt tttttgttta
    13501 tcctcaaatc cgatccaacg attgaaaccg tccaaagcta aaagcgcgat catgggaccg
    13561 acagctacaa aatgacgtaa aaaaccgacc gatgccacat tccctttcca gtagaaatat
    13621 acatgcaccg caaacatcaa taccacagaa gtattaagta gagacagctt gcgtaatctt
    13681 atatccgagg ccaactggaa caaaagaaaa acaaaaatca caggacccgt aataaaaata
    13741 aatcgctgaa aataatgttc aaaaggtccg ttttgatata acccgtgccc aaaagctttg
    13801 gattgggtca acagatacag cgggtctccc gtcgaaatca taccggcgat ttgataaacg
    13861 gctaaacccg taccaagcag caataccggt atccatcggc gttcacgcag ccagggaatc
    13921 gctatcaaag gaagtataag caccgtctcg gaacgcgcca ggggcaccat cgagaaaagt
    13981 aaagcagctt gccataactt attgcgataa taaaaatata atgcgatggc aacaaaaaaa
    14041 gccgccagcg gttcggcgca taaactaaat gatgtcgtta agaccaacgg ttgaaaaaaa
    14101 acaaaaagta caaccatcca agcatagggc cgtccacttt cctgcgcggc cttataggta
    14161 aaaaaacatg tcatcacggt caaaagagct accccggaag cgacatacca atacccaaac
    14221 tgagccggaa tcgaaaaaag taaaatagcc atcgggcgcc cccagatatc caaaaaatat
    14281 ccgggcttca caaacgctgc ctgcgccata aaataacgcc ctatgttatc atcatcatac
    14341 gggcgcggtg aattgaccat ataatagata agtgtcaaaa caacggttga cagagataat
    14401 atcaaccatg ttttcgtttg attccaatcc gccaatccgg acgatttcaa tggagatgta
    14461 tttttgattt tactttcttt atttttcata acatgtttta agtgaattaa aaagttttga
    14521 ctaattgaat gcaggccgtt cctgtgctcg acggttcaat ataaaatctc catcctgtcg
    14581 tgctttgagc cgtggtcatg cgcttatatt gacgcgtcac atgtacgata ctcatcacat
    14641 gatttacaaa caaaccaaaa tacatatatg tagaaacctg acgaaatcgc ttcgtatcgg
    14701 cgcgcatctt atcgtacttc cgacgatcag aatctttttg ccactgccaa tagttggcat
    14761 cggtctcatc atataaaaac tccgtccccg tggcgcggcg ctgcgtttca ttgtacgtgt
    14821 agatatcacg atacttcccg atattcacat aatattttgc cggtttagtt gatcggatat
    14881 ctacgccggc ttttcttgcc gccaatgcac gagcgtcttc tttaagccat ccgctgtacg
    14941 aacgcgtata aaaattagta gcccaaaaca atacttccgt cgcggcaaaa tacttaccgc
    15001 ttccccattg ctcaaatttc caatccgata aatacaattc acccgtaccg ggcgctaata
    15061 aggataatat aatcgctttc ttttttgatg ggccagatgg cctttgctct tgcgccagta
    15121 cgtcaaaact atacgacaac atcagtactg tacacaacaa aaaccgtaac ctcatgctga
    15181 caccccttct tttgtcggaa acatcaaacg caccatcgta ccattcgttc cactcgatgt
    15241 aatggttata tccccgtcat tttcatctac aaattttttt acgatggcta aacctaaacc
    15301 ggagcccttt tctttcgtcg taaagaacgg atcaaagatt ttctctttta tattatcctc
    15361 gatgccatgt ccgttatcct cgacggtaat ttcgatcatc gcattgacac gctgcgacac
    15421 gatacgcaca cggggcaaca cattacctga ctgcatggca ttttcgatca gattgatcaa
    15481 aatttgccgc aagtgccccg gatccgcata aacttgtttc tcattcaact cgacttccca
    15541 ctgtaccgat ttttccgtcg taagggccga aagattgatt cgaatttcat caataagttt
    15601 ggacaaatca cagaccatcg gttgcggctc tttaggtcgt gcgtagtcta aaaaatcatt
    15661 cacgatttgt ttcattacat ccaactcgcg caagactttg agaatagctt tcttgcggtt
    15721 atcgccgtcc ggaatttctt tattcagtaa atgggcatac aattccatcc cgccaagcgg
    15781 attgcgcaat tcatgcgcta cgccggccaa catagttttc atatactgat cgcgtttaag
    15841 gatattttca cgcatgcgat ccatagtacg tcccaaaaaa cccacctcat cgccggtttg
    15901 aatagcaatt ttgcgatcaa gctttccgct gccgatatgt tccgcttcga ccataatctg
    15961 ttttatcggc gtagaaatag agcgtgaaaa ataaaacccg atagctacgg acacaaccac
    16021 accgatgata ccgacggtga ttaaatgcca ttcgatgcgc ttcatcaaat ataagaacgt
    16081 cgcgctgccg tccacagcga tgacgccatg ggtctgcccg ttcaccgtga gcgccacaaa
    16141 ggctgtttta tataattttc cgtcatatgt ctcaaacaat atggagtgcg atggcaatcc
    16201 ggcggccgcg cgttcaaatt cgtattgatt aaacttgaga aaaggatacg catacccgat
    16261 acgtacacct tcctccgtat ccataagact ccggccgttg ggatcaatga tataaattcg
    16321 ctcaacatcg gtcgcatcgc gcagatccgt caatttctgt ttaaacgtac ggtaagtgcg
    16381 cgaattttca tcgccggact caagcttgga aaccatttcg gtatgtatct gaagtttggc
    16441 ggcctgcgcg acggccagca gtttcttccc gagttcttca tcaagactgc tctcggtgta
    16501 ccaataatga ataattccgc tggcgctgaa tatggtaagg caaaacgcgg caaacgtaac
    16561 gatgagttta ttttgaattt tagcgcgtat tcggcgtaac atagatgcgc aatctatccg
    16621 atttctccgc taatggcaag cgatttaaaa gtgcagtacg cattatcaga ataataatac
    16681 ctatcctcgc gggtgaaatg tcttgtgcac ttctttgagg tattcgcgat caatatgcgt
    16741 atagatctgt gtggtcgaga tatccgaatg accaagcaac tcttgcacga cacgcaaatc
    16801 ggcaccgccc tcaagaagat gcgttgcaaa ggagtgacga aatgtatgcg gcgtaacctc
    16861 tttctgaatg ccggccatcg tgacgtattt ttttaagatt ttccaaaacc ccatccggct
    16921 catccgctta ccttgcgcat tcaaaaaaag tacttcgtcc gccgatccgc tcgccgcaag
    16981 gtgcggacgc actttctccc gataataggt tacccaatat cccgcggtat cgcccatagg
    17041 cacaatacgt tctttggagc ccttgccaaa tacccgaacg taatccgaat aaatatccaa
    17101 ctgcttgatc gtcagtagtt ccgagatgcg cataccgcac gcgtatagca attcgatcat
    17161 tgcgcggtct cggataccga gccgggacga ggtgtccggt tgctcaagca acgtttcgat
    17221 ttccggcact tccagtaccg aaggtaaggt tttttcgatt ttgggaaggt caatcaattc
    17281 ggcaggatcg ttttctagaa tttcttcctg taaaagaaat ttatgaaacg tacggatggc
    17341 ggataagtta cgggcgatac tttttgctgt aagcccaagg ccatccagcg tttgcacaag
    17401 ctcacgcacg tcttgcatgg taacatcttc gatttttttt cgttcgcgtg atcccatgac
    17461 aaacttcaga tagcgttcca aatcgttacg ataagaagcg agcgtattcg gtgaaacgtt
    17521 ttgttcaacc gaaagataat ctatgaagtc ctgaagatgg tgtttcataa tcgcttattt
    17581 acacatgaaa agcatcccat gcgattgtaa tcaggttttt taagagatca aaacaaaaac
    17641 aaggatgggt agtctattct acgataatcg tttggaaaag aagatgtaaa cagaattctt
    17701 ggaatgcttt acagcttgac gaaccgacct tgcaacacgg caccgtctca tttgtaaatc
    17761 tgcgtcatca cacgatcatc gtgatgatac gccgacaagc agcagaatgg tataacggtt
    17821 tttagcctaa gttagaacgc acttccatgt ggcggcagac taatacttac ttaattagcc
    17881 tgcgctttat ctttgatata gctggattca aattcaaagc tttgcagttc ggccaacgca
    17941 tcattgagca gaacgacgcg ctcacggtat ggaagaaatg tacttttgaa gccattgata
    18001 atgatgttgc aaacatcatc aaacgacaga tcaaaagttt tcgccgcacg ataaatttct
    18061 ttggttacgg ttgtaccgga aatcaatcgg ttatccgtat tgaccgtgac gcgcaaaccg
    18121 aaatcataat aggtgcgcaa cggatgcgac gaaaaatctt ttacggcttt ggtctggaca
    18181 ttgctcgtaa ggcaaatctc aagcggaatg cggtgatcat tgacatagtt aagcagatcg
    18241 ccgtcctcga taaggcgtgt accgtggccg atacggtgtg caccgcagtc atggatcgct
    18301 tgtttgatcg aatccggtcc ataggcttcg ccggcgtggg cggtcgtatt gacgttattt
    18361 ttgagtatga gctcaaatgc cgcacggtga tcttttgccg gataactgtc ctcttgaccg
    18421 gcaagatcaa aggccatgac ttttttccct ttgtaggcga cggtcaattc ggccagtcgc
    18481 agagaagttt cagggctgat attgcgcata ccgcaaataa taatacccgt tttgatttta
    18541 tatttggatt cggcgcgttc gagtccacgg atcaccgcat ccacaatctc ggtgagacgc
    18601 agtccgcgat tgatgtgcag gatcggcgaa aaacgtactt ccagataacg tacgttttct
    18661 ttggccgcat cctccgccag ctcaaacgcc gtgcgttcaa tcgcatacgc ttcctgcatc
    18721 acgctgagcg tgatggcaaa acctttgagg taatccacca ggttttcaca cttatcaccg
    18781 acttcaagat aagattttaa tttgaccgga tcttcgaacg gcaattgaac attttgttct
    18841 ttagccaatt cgatcagcgt ctcgacgcga ttactgccgt caaggtgaca atgaagttcg
    18901 gttttaggaa gacggtgaat cagttcttcc gttacttcga tgcgattgag ttcttttttt
    18961 gccatggtat ccgcctataa aaattcccgc ttgcgcggga atttggtttt ttaatttttc
    19021 tttaccgcct gcaatatcat atcggctgcg tcgttgagct cattggccac gagcatcggt
    19081 attcccgatt ttctcagaat ttctttcgct tcttccgcgt tggtgccttt gaggcgaacg
    19141 atcaacggta cgcggatatt gacattcttt gcagcttgca cgacaccgtt agcgatacga
    19201 tcgcaacgca caataccgcc aaaaatattg atgaaaatgg cttttacatg cggatccgac
    19261 aaaatgattt taaaaccatt ttctacggtc tgagggttag caccgccgcc gacatccaag
    19321 aagttagcgg gattggcacc ggccagtttg atgatgtcca tcgtcgccat ggccaaaccg
    19381 gctccgttga ccatacaacc gacattgccg tccagtttaa cgtaattgag attgtattta
    19441 gacgcttcca cttcgagcgg atcttcttcg tcaatgtcac gcaaggcggc aagatcggga
    19501 tgacgataca gagcgttatc atcaaaatta attttggcat caagcgccac gacgcggtta
    19561 tctttggtaa tgaccagcgg gttgatttca aacagtgacg aatccgttcc gataaacgca
    19621 tcgtacagcg ccatggcaaa cttcacaaaa tttttgtgaa tatcgccttc gagcccgagg
    19681 gcaaatccga ggcgctgcgc ctgaaacgca cgaaagccga ccgtcggatc aacgtattct
    19741 ttgatgattt tttcgggatg ctcctccgcc actttttcga tttcgacgcc gccttcagta
    19801 gacgccatca tcacatattg ggagcgtgca cgatccaaca ccaagccgac atagagttct
    19861 ttggcaatat ctagaccttc ggcaaccaaa acttttttga tcagttttcc ctgatcgccg
    19921 gtctgatgcg tgacgagcac attacctaac attttttcgg catattctgc aactttatta
    19981 atatccggtg tgaattttac accgccgtgc tgcaccactt cgttgcgctc tttggcttta
    20041 taaatagtac ctttaccgcg accgccggcg tggatctgcg ctttgacgac ccatgctccg
    20101 ataccttgcg ctttcaatgt tttcgcagca tctatcgctt ccgttacggt agcagcagga
    20161 attccctctt gaatcggtac tccgtatttc tttaatatgg ctttaccttg gtactcatga
    20221 atattcatgt aatcatatcc tccgatgaac gtggaatagt tttttgcttt tgaattgcgc
    20281 gcaatatata aaatcggctt gtataatcaa aaagaatttt tgctttatac caaatattca
    20341 aaatactaat catttcattt tcacatcatg tcactttatg agtcttaact aaaacaatac
    20401 atcttattat taatcttata cttgcaataa actcggttta aattgtactt gttaacatca
    20461 gatgacaact atcatcatag tctcaaccca aactttcaat ttgcctttca ctaaatattg
    20521 atttatatta agcttatgaa tttttaatcc taacttatta cgtttatatt ttacagggat
    20581 tcccatgaag cagaaatatg atgtggtggt aatcggagcg ggatgtatcg gtgccagcgt
    20641 ggcgagccac ctcggagaac tcggttgtgc taatgttttg gttttagaaa aagaaaaact
    20701 tatcggtacg ggctcaacag ccaaatgtgc aggtggtgtt cgcgctcagt tttcaacgcc
    20761 gatcaatatt tacatgagcc actacagcat tcgcgaattt gaaacattgg aaaaagaata
    20821 cggtatccag ttcaatcaat gcggttatct gtttgttgtt cgaaatgaag agcaaaagga
    20881 acgttatttg aaaaatatgg aaattcaaca aagttacggc gtagatgtac ggtttatttc
    20941 caaagatgaa atcgcagtgc ttgcacctaa ttatgggttg gacaacgtgc tcggaggtac
    21001 atttggcgcc aaagacggct tgattgatcc ttcgatgacg gtcgatgctt atttcaaacg
    21061 cgcgcgtaaa cataacattg attttgagac cgaaacacct tgcaccggat ttaaaacctc
    21121 aggtaatcgg atcacgcacg tcatcacacc caaaggtgaa gtggcatgcg acatggtggt
    21181 caatgcagcg gggccttttt ccaaagaact cggaaaaatg ctcggttttg aaattcccat
    21241 cgaacccgta cgccgcatga tcaccaccac cggtgaatta ccttttgtga ccaataaatt
    21301 tccgatggtt gtggacgtca cgaccggtat gtatatgcac aaagaaggta atggactttt
    21361 aatcggtctc gctaataaaa acgaaaaacc gggatacgac gaaaacattg atacggaatt
    21421 tttagacgag atgttgacga ccgctttgga agtcatgcct tatctcgaaa atgcagaaat
    21481 caaaacacaa tatcccggaa cgtggggcgg tttgtatgaa gaaacaccgg atcatcatag
    21541 cattctcagt tatgttccgc agtttagtaa ttttattgtg gccggcggct ttagcggtca
    21601 cggcctcatg catgcaccgg ccgccgggcg cgccatcgca gaacttgtta ccaaaggcac
    21661 atgcgacacg tttgatctga agccactgcg tttcacgcgt tttgccgaag gcgacctgac
    21721 caaagaacga aatgtcattt aaaagaaaaa cggttctctt aaaataatcc actatatcga
    21781 caccaatctg aggatggcgt tatggcaacg aaaaaagcca aaaagaaaaa tatgtttgac
    21841 gcgataaaac cggtgatagc caaagcgaaa acgttcgttt tgacgaccca cgtcaaccct
    21901 gacggggatg gtctcggcag cgaagcggca atgtatcttt tcctgaaatc actcaaaaaa
    21961 tcaccgcgta taatcaatac aagcccaatc cccaaagctt ttcagtattt gaattatggc
    22021 aacatcatag aaacttatga acctgccaaa cacgacaagg tcatacaaaa agccgacgtc
    22081 gtaatggtgc ttgatatcag cgtttccaaa cgacttgatc gaatgcaaga ggcagtcgtc
    22141 caggcgccgg gcgcgacgat atgtattgat caccatttgg ataacgatgg tttcgccgac
    22201 cacctgtgta ttgatgaaaa agcaccggca acagcagaac tggtatataa tttcattcgt
    22261 tacttcaaag gtaagctcaa ctttaaaatg gcccaagcgc tgtacacttc tctgctcacg
    22321 gacaccggcg gatttcgttt taattctacg cgcccagaaa cgcatcacat ggccgccgag
    22381 ctccttgcta tcggtgtgaa acccaacgat atttattctc aaattttcga acaaggccgc
    22441 atgagttccc tgaaactgct cgggcgggct cttgatcgca tgaaaagtga atgcgccgaa
    22501 ggcatgcatt ggacttatct cacaaagaaa gactttcagg aaaccggtac gacgaaaaat
    22561 gataccgaag gatttgttaa ctacgctctg agtcttgaga attcgatgtt tggtgtattt
    22621 gcttacgaaa ccgatgaagg atttatcaag ttcagcctgc gttccaaagg tcatgttgac
    22681 acccaagctt tttccaaacg ttacggcggc ggcggtcaca aaaatgcgtc gggcatttcg
    22741 ctcaaagaac catttgatgc cacgctaaaa aaaatgattc ccgaaatggt caaatattac
    22801 aacgaaacct acggtaaaaa ataattatgc acgtatcggc caaatattcc aagctcaagc
    22861 actggaaaag cgaattaacg tgcgtgttta ttgccaaaaa cgaacctgta aatttttgga
    22921 aaaatgaact tgaagatatc tacgaagaca cgattcccaa gttactgaag tccaaagaat
    22981 ttggcgcgga agaagaaaaa gtatttctcc ttttgggtaa tgacaaaacc tcaagtaaac
    23041 gcattgcact tatcgggtta ggcgaataca aagatctcga gccggaaact ctgcggcgaa
    23101 cggcagccaa agccgtgcgt acggcactcg ctatggaata ttctaaaatc acgctcgcct
    23161 ttcctgcgca tttgcggcaa aaaaactgga ccgatgatcg catagctcaa gcacttaccg
    23221 aaggcgcttg ccttgcgcat tataagtatg ataagtacca tacggacaaa aagccgcaag
    23281 accggcatcg cattacagag ctaacgctta ttgccgatca tgagccgaca gccgccctgc
    23341 aacagggtgt tcgggccgga gaaatcataa gcaatgcggt caattttacc cgtgatctag
    23401 gcaatgcgcc gggcaatgaa ttgcctccgc gtgaattagc cgaccgtgca acggcgatgt
    23461 gtcgtacgca caaaatcaaa tgtaccgtat tcaatgagac caaaatcaaa tcgcttaaaa
    23521 tgggcggcat tcttggtgtc accgccggca gcgccgaacc tccgcggctg gtcaccatgg
    23581 aatacaaacc cgctaagccc aaaaacacta aaccgatcgt actcgtcggc aaaggattaa
    23641 cattcgattc gggcggcatt tcgatcaaac cggcggacca aatggatcgc atgaagtttg
    23701 atatgtgcgg cggtgcagcc gtcattggta ttatgcaagc aattgccgaa ttgaaacttc
    23761 cgttgtatgt cgtaggcatc gtcccctctt ctgaaaatct taccggcagc cgcgcttata
    23821 aacccggtga tgttttgact atgtattccg gcaaaacggt tgaaatcatc aatacggatg
    23881 ccgaaggacg attgatcctc gccgatgcgc tggcttacgc tcagcgctac aaaccggaag
    23941 tgatcattga ctacgctacg ctcacaggac attgtgtgat tgcattgggt atgcatggcg
    24001 ccggtctttt tgccacagat agtaatatca aacaaaagtt gatgcacgca tccgatgcaa
    24061 gcggcgaaaa aatatgggaa ctcccgctgt ggaaagaatt tgacaaacaa atcaaaagcc
    24121 atatagccga catcaaaaat accggtggtc gcccgggcgg cgcctccacc gcggcggcat
    24181 ttctcaaaca ttttgtcggt aatacgccgt gggcccattt ggatatcgcc ggcaccgcca
    24241 atagtgaaga agaacttcct tacacggatc gcatttctgc gacaggattt ggcgtaaggc
    24301 ttactgtcga aatgttgcgc cactggtgat catgtcggat atcgtactca cggataatga
    24361 cctcgcttta cttgctcaaa tcggcaagtc acccgaacaa ctatcggccg aaatcgaatt
    24421 tttcaaaacc ggatttccgt ttgtcgaatt ggatcgtccc tgcaccatag gcgacggcat
    24481 aacagtttta tccgacagta cgcacgatgc cttaataaaa aaagccgacg atgccgcggc
    24541 ggcagggcgt gtaaaaaaat ttgttcctgc ttccggcgcg gctacacgca tgttcaaaag
    24601 tttgatggcc gttcattatg atcgtatgct tcaacaaaat acctctcacc ccgacacgcg
    24661 ttttttaaaa acattttttg atcggcttac ccagtttgct ttctatgatg agttagacgg
    24721 tgttttggca aaggacggtt tgtctttgga aaccttgcgc caaacacgtt cctattcgga
    24781 cgtattatca tatctgatgc atgatgtcgg tttggactat gcccaccgac ccaaggcgct
    24841 cattcctttt catcgttatc ccgaaggtgc tcgtacggct ttggaagaac atttgaccga
    24901 agctctggct tatgcgacag atacggatca gaatgcgtac gtgcatttta ccatttcaag
    24961 cgaatatcgc caacttattg attcgcacat tcaaacggtg cgttctcgtt acgaaacgag
    25021 cggccgtcgc attcaaattg aaatttcaga gcaaaaacct tccaccgaca cccttgctgt
    25081 tgatttgcac aataggcctg tccgcgatca ggatggtcgt ttacatctgc gaccggccgg
    25141 ccatggggct ttgatcgaga accttaacga tctggcaggc gatattgttt ttatcaaaaa
    25201 cattgataat gtcgtacctg atcgtctcaa agagccgaca ttccgataca aaaaaatttt
    25261 atgcggtcat ctgattgaaa tgcagctaga aatttttggt tatcttaaaa aactaaaaaa
    25321 taatcctccg aattccgata cggtcacaga agccgaatcg tttgccattt cacgacttgg
    25381 catgcaagca agttcgacga tggatatccg cgagcggcgt gataatttga tgcaattttt
    25441 aaaccgcccg atccgtgttt gcggaatggt gcgtaatcaa ggcgaacccg gaggtggtcc
    25501 gttttgggtt agagaaccta acggaaaccg ctcgctccag atcgtggaaa cagcccagat
    25561 tgatcttcaa tggcccaaac aaaaactcat tttagatgac tctacacatt ttaacccggt
    25621 agatatcgtt tgcggcgtcc gcgattttga aggaaagcca tttgacctgc atcgttttat
    25681 tgatccccat accggcttta tttccaaaaa gtccaaagat ggacgtgaaa taaaggctat
    25741 ggagttaccc gggttatgga atggggccat ggctaagtgg attactattt ttgccgaggt
    25801 gccgctcatt acgttcaacc ccgtcaaaac gatcaatgac ctattaagac cggagcatca
    25861 accataaagc aaaaccttgc ttttttagag actttggcgt attatcgccc gcaacgtatg
    25921 aatgtattat catttgatat atataaaatt gtaaccacga atgtggcaga aattttcttc
    25981 caggtttagg ggcgcagcga tgttatctga tatcggtctg cccgcgaata ccacggtgca
    26041 aatccgtgat ttatatattt gttcttttcc aatagttttg gaaatccgat cctgtacgtt
    26101 aaggaagaac ctctgcccta ttcgatagaa taggacacgt atgaaaaaag aaattatcat
    26161 caacgcgacc gatgcggaaa cgcgcatcgc catcgtggag gatgaacggc tcgtcgaaat
    26221 ttttgtcgaa cggccggaaa aaaatcgaat ggtcggcgac atttacaaag gtcgcgtcgc
    26281 caaagttttc ccgggcatga aagccgcctt tgttaatatc ggcatgaagc aagatgcctt
    26341 tctgcatttt tccgatatcg gtgataacac gcacgaatat gccgtcatca atgatgccga
    26401 tgacgcggac gataacgaag cgccggtcaa accttttaat cacccccgta ttcataatca
    26461 tgataaccac ggacgctttt tacgcgtcgg tcaggaaatt ctcgtccagg tcatcaaaga
    26521 gccgatttac aacaaaggcg cccgtgtaac ttcggagatt tcgatacccg gccgatttac
    26581 cgtgctcatc cccaacgacc ccaatgaaat gatcggcatc agccggaaga tcaatactgt
    26641 tcccgaaaga aaacgactcc gccgattggc caaagaagtc aaaccgcggg attttggtct
    26701 gatcgtacgc accaatgcgg aagagcgcga tgaagaagta ctgcgtaacg atattcgcaa
    26761 ttgtttggac acctggcacg atattgatac caaagctcaa aaaatgtccg cacccaatct
    26821 cgtgtataaa gatatggaaa tggtctccag tgtcatccgc gatctttttt ccaatgatgt
    26881 ctcccgggta cttattgatg ataaaaaatt atacaaatcg atttctaact atatctcgac
    26941 gacatctccg caacttggta atcgtatcga actctatcgc agtaacaagc cgatatttga
    27001 tttgtacaat atcgaacagg atctgcaaca aagtatcgag aaaaaagtac cgctcaaaaa
    27061 cggcggttac atcatcatcg aacatacgga agcgctcatt tcggtggatg tcaacagcgg
    27121 caaatacatc ggacgcggtt cttatgaaga aaactccatg gccgtcaata tggtcgctgc
    27181 gcgtgagacc tgcaggcagt tgcgtttacg ggatatcggc ggacttattg ttattgattt
    27241 tatagatttg cacgacgagc gcaacaagaa gaaacttttt gaagaaatga aaaaagaact
    27301 tcgaaaagac cgcgccaaat tcagcattct tcctatggac gaatacggtc ttattgagat
    27361 gacgcgcgag cgcatccgcc cgagtattat gttttcgctc agcgaacctt gtccggcctg
    27421 tagcggtacg ggtcgcgtgg gcaatcgtac cacgctattt gataaaatcg aacgttgggc
    27481 gcgccgttac aaagaaaaaa acagcttttt ctccaaactc gaattcgttg ttaattccga
    27541 tattttggag caggtcaagc ccaaaatcaa aaatatgcgt tggcgttatt tgatttttag
    27601 ccgtgtcacc ggcgaacagg aaatccgtat ggacgaattt cgtgtgctgc aaaaaaatga
    27661 ggatattacg gaaatgtacg gttcctaaga atttcaaatt atccactttt agctgatacg
    27721 catgttttta cacgccacta caatatatgg accgctgacc acgcgccgat tgggtacatc
    27781 gctggggatc aatctgcttc cggttgggcg taaagtgtgt aattttgaat gtgtgtactg
    27841 cgaatgcgga tggaccgata cgtccgtcaa agatgcggtc ccgacgttgg aggcgtttac
    27901 cgaggcactc gagtccaaac tatcggcatt acggcaacaa aatcagaaca ttgaccacat
    27961 tacttttgcc ggcaacggcg aaccgacttt gcacccgaat ttttcagcca ttattgacac
    28021 cacccgtgaa aaacgtgaga aatactttcc gaatgcccgt attgctgttt taaccaatgc
    28081 aacgcgtgtt cacaaaactg aaatattcga cgctctcaaa aaaaccgacg accccattct
    28141 caaattggat gccgggacag aaactatgtt tcagcggatt gatataccgg ataaaggaat
    28201 ttcgctggac ggtataacgg aaactcttaa aaaatttaat ggcaacctaa tcattcagac
    28261 cctattttta cgcggcgaac accaaaacac ggtgattgat aatactacgc cggaagaagt
    28321 tcacgcatgg cttcgtcgca tcgaagctat aaaaccgcga aaagtaatgg tatatccgat
    28381 tgatcgcgat acaccggtta aaacactcga aaaaataagc gcaaccgaat tagaaaaaat
    28441 agctgctcaa gtccgtacgc tcggtatccc cgccgaggta tttggttagt ctataacata
    28501 aggaaaacaa tgtccgctat cgaagatacg caaggtcgag acgattttcg taaaatgccc
    28561 attcaacggg tcggcataaa aaacctcaaa tatcctctga cttttatcaa tgccgcaggc
    28621 cgacgcactc caaccgttgc catgtggggt ttggctgttg atttgcccca cgataaaaaa
    28681 ggcacgcaca tgagccgctt cggcgaaatc ctcaatgcgt ggcaggacgg attatccatc
    28741 gaaagcatgc cttattttat gaatatgatg cgcgaacggt tgcacgcgga tcgcgctttt
    28801 gcaagagtga cttttgcata ttttatcgaa aaagaagcgc ccgtcacact caccaaaagt
    28861 tttatggatt ataccgttgc gatcgacgcg tctagcattc acgataaaat gacgttgtcc
    28921 ttggaagtac aggtgccggt caccacgttg tgcccttgct ctaagtctat ttccgaatac
    28981 ggtgcacaca accaacgcag ccttgtaacc gtaaaagccg agtgtgaaac ggataatttt
    29041 ctcagtatcg aagaattggt ttattccgtt gaaacatgtg ccagcagcga gctctatgtc
    29101 gtactcaaac gcgaagacga aaaatacgta accgaacgcg catacgatca tccggcattt
    29161 gtcgaagacc tggtacgcga catcgccatc aaactctcgc aagatgctcg tatcgtgcgt
    29221 taccgtgtag atgtgactaa tttagaaagt attcataatc atgacgctta tgccgtgatc
    29281 gaatcccaaa gcgtttcatc ggtactatct gataacgggg ccaagcatca tgcatcgccc
    29341 gaacaacagt cttaacacgg agtttgcatg cgcagtctat tccttctgat tttgaccggt
    29401 ctgatgatat cggttaacag ttgccattgg tttgtagtaa agccgcaatt tgacggccaa
    29461 tcggccatga attacctgaa aaaacaatgc gaaatcggcc cgcgaaaccc gggatctgaa
    29521 ggccaccaaa aagcgctcga atatatgacg gaagagcttc gtaaatattg cgatcgcgta
    29581 gatcatcaga aatttcatta cacggacaaa cgcgatacaa ccaaaaaatt tgaactcacc
    29641 aatatcatcg cgtccatcaa tctcaatcct aaaaataaaa agcgcgttct tctttgtgcg
    29701 cattgggaca cacgcccatg ggccgataaa gatcccgatc cggccaatcg catgaagccc
    29761 atcatcggcg ccaatgacgg agcgtccggt gtagcggtat tacttgaaat ggcgcgtacc
    29821 ataaagcaac agccgctgga catcggtgtg gacattattc tttttgattt gtaagattac
    29881 ggcgatcaca attgggaatc caaaccggat agtctcaatg cttattgtat cggatcggaa
    29941 tactttgcaa agaataataa aacatatttt ccggaatttg ccattctgct tgatatgatc
    30001 ggtgataaaa atctggattt accgattgaa caaaattcgt acaccggcgc aaaggacatt
    30061 gtgaataaag tctgggatgc cgccaaacgt cttgaaaaac ctgcctttcg caaaacgatt
    30121 gaaagcaatg tttatgacga ccatataccg ctgcttcgga tcggcatcaa atgtattgac
    30181 atcatagatt ttgattatcc ggatgacagc aaccgttacc accacacgat ggatgataca
    30241 cccgataaat gcagtgccga gagcctgcaa caaatcggcg atgtattagt cgaagttttg
    30301 tacaacgaat aatcacggag catctatgga ttatacaacg atacgtgtcg aacatcatgc
    30361 gcgcgaagcg cacgtgacgc tcaatcgccc accggtcaat gtcatcaatc tgtccatgat
    30421 tgatgaacta aacgtcgcat tagataaaat cacaagcaat ccggatgtaa caacgattgt
    30481 gtttcgcggt gcaggtgata aagcgttttc aaccggcgtt gatatcaagg atcacacgtt
    30541 ggacaagatc aacagtatgt tgaaatcgtt tcatcatatt tttcgcactt tggctaattc
    30601 cgataaaatc accgttgctt ctgtccacgg ctattgcctc ggaggcggta tggaattggc
    30661 tactttctgt gatttcgtaa ttgcatccga ttcgactatt tttggccaac ctgaaatcaa
    30721 agtaggatgc taccctccgg ttgctgcggc atggttacca caaaaaatag ggttacgtcg
    30781 cgctatggaa atttgcatgc tgggtgaaaa catcaaggcg cacgaggcct taacgctcgg
    30841 tttgatcaac aaggtggtgc cgcccgatga actacgatct gctaccgata cgcttttaca
    30901 aaatctgaat gccctcagcg gtaccgtact taaacaaacc aaacgagcat tgctggccgg
    30961 cgcggataaa cccttcacta ccgcacttga tacggctgaa aacatatacc tccaagagct
    31021 ggtcaaaacg cacgatatcg aagaaggtat taccgctttt atgcaaaagc gaaaaccgga
    31081 atggaagcat cgctgacaag ggaaattggt attgaatgtg aaactctttt ttgatacttt
    31141 gccccgctta attcgaagaa tatactatat tacatacatg atcaattaag gagcgttact
    31201 catggcaaaa gtggatatgg tcatgcccca gatgggcgag agtattgccg aaggcaaaat
    31261 tctgaaatgg cacaaaaaag tcggcgataa gataagcaaa gacgaaaccg tccttgaaat
    31321 cagcaccgac aaagtggact cggaaatccc ttcgcccgca tcgggcatta ttgctgagct
    31381 catcgttcct gaaggtacga ccgtcgctgt tggcgcattg atcgctcgta tcgaaaccga
    31441 tgctaacgcc tcggtcgcat cgccggcacc tacaccttct cctaaggttg agtccaaaac
    31501 agctcctgta gcacaaaaca cgcctgcttc aaagcccgct tccggtggag cctcggtaga
    31561 tatggtcatg cctcaaatgg gtgagagtat tgccgaaggt aaaattttga aatggcataa
    31621 aaaagccggc gacaaagtgg ccaaagatga aactgtcctc gaaatcagta ccgataaagt
    31681 agattctgaa attccttctc cggcatccgg cgtgattact gaaattatcg ttcctgaagg
    31741 tacaaccgtg gccgttggaa ctttgatcgc acgtattggt gccgaaggcg ctacggtcgc
    31801 cgcttcaaca acggccgcac cgacgcccaa aaccgaagaa aaaatgcatt tttctagttc
    31861 tattgaggac ttccagaaac agaaccaaat cgcagcgaca gcggcgactc ccgcagctac
    31921 tgtaaatgcc ggcggcggca tggtcaaagg cgagagcaat cgtttttatt cgccgctcgt
    31981 acgttccatc gctaaagctg aagggctttc gatgcaggaa ctggattcga tcgccggaac
    32041 cggacttaat catcgcgtaa gcaaaaatga cgttctcgcc tatttagcta aacgtcccaa
    32101 aggcggcgtt cgtcctgctg tcacgacgac aacatccgca tctacagcgg ctgcaccggc
    32161 gaaacaagtt tccgacgtta aatacggata caataaagaa cgctcggaac tcatcgaaat
    32221 gaataatata cgccgcatta ccgctgaaca catgcgccgg agtattgaca cttccgcaca
    32281 cgtgtattcg atgacggaaa ttgatatgag ccgcgtcgta cgttttcgcg ataaatacaa
    32341 atcggctttt gaaaaacgcg aaggattcaa acttactttt accccgtttc tcattgatgc
    32401 gatggtgcgc gcgatcaaag atttcccgat gatcaatatc tccgttgacg gtaataccat
    32461 cgtgaagaaa aactacacca atgtgggtat ggcggtatct atcgaaaaca atacggcgct
    32521 aatcgtaccg gtaatcaaag atgccgatgc gatgaactta gtaggcgttt gccgcgcaac
    32581 gaatgatctc gctttgcgcg cacgctctaa aaagcttcgc cccgaagata ttcaggatgg
    32641 aacgattacg ttgaccaata tgggcgtttt cggtacgacg gtaggtttcc ctatcatcaa
    32701 tcaacctcag gtagcaatta tgggcgttgg tacgatcaaa aaacgcccag tagtgatcac
    32761 taccgatgat ggtgaagata tgatcgtaat acgcccaatc atgtatgcat cgctctctta
    32821 cgaccaccgt gtcgtggacg gcgctctcgg cggacagtat ctgcaacgcg tcgcatacca
    32881 tctcgaaaat ttcgacgaaa accagactat ttaacatttg ttatgtttca aaaaaactcc
    32941 cggcattacc tgtcgggagt tttttattgt gatgtgttat gaataaacga ttaaacgtaa
    33001 gcttatattt tattgctgat gtcggcatga tacccgccgg caaaatatgt tctgtagtcg
    33061 aagaagctat tcaaggcggt gtcacgttcg tacaactgcg agcaaaaact ctttctgtca
    33121 atgcgatgat tgcgctaggg cgaacaattc accaagtaac atatagcaaa aatattccgc
    33181 tgatcatcaa tgatcgtata gaagttgcca cggctatcgg tgcggaaggt atacatctcg
    33241 ggcaagagga tattaccgtc ggagcagcgc gtaagatatt aggcgattcg gcaattattg
    33301 gaatttcaac ccacacgcaa caggaagcgc aaacggcaga atccgcgggc gcggattata
    33361 tcggcgtcgg caccgttttc ccgacggcat ccaaaaaaga tatagtcggc attatcggta
    33421 caacggggtt aaaacaaatt cgggatttgg taaagttacc tgtagtagcg atcggcggta
    33481 taaaccaaaa taatgttaaa gaagttctcg catgcggagt tgatgggatt gccgtaatat
    33541 ctgcgatcgg tctatccgta aacccccgtg aaacaacccg cgcactcaag gatctcttga
    33601 cagtcaatta gattttccgg tgatcattct tccaaatcta aaccggcctg ctgagaagtt
    33661 tttcttggtc gaatacgcag atgatcgtag gccaatttgg taacttctct accacgcggt
    33721 gtacgcttta ggaaaccttt ttgaataaga aacggttcgt aaacttcttc gatggttccg
    33781 ctttcttcgc cgacggctat ggcgatatta tcaataccca ccggtccgcc attaaagcgt
    33841 tcgataatat tagataaaat gcgtttatcc atttcgtcca aaccatgctc gtccacttca
    33901 agtgctttta aagcataatc ggcgatttct ttatcaatga tttttttacc gcgcacttgc
    33961 gcaaaatccc gcgcacgttt taaaagccga ttggcaattc gcggcgtacc gcgcgaacgg
    34021 cttgctattt cattagcacc gctggcgtct atgtccacct gtaaaatttc agcggaacgg
    34081 gtgacgatgt gcgccaaatc tttatcattg taataatcca accgcgaggt aataccgaaa
    34141 cgcgaacgta atggtgcggt gataagtcct gcccgcgtcg tcgcaccgat caatgtaaaa
    34201 tgcggcaacg tcaactgaat ggtacgcgca ctcgggcctt tatcaataac aatgtcgagc
    34261 tgaaaatctt ccatggccga atataaatat tcttcgacga tcggattgag acgatgaatt
    34321 tcatcaataa aaagcacatc acgttcttcc agattggtca gaatgcccgc aagatcaccg
    34381 gctttgtcta agaccggacc ggaggttatt ttgatatgca cacctaattc gtgcgccacg
    34441 atatgagata acgtcgtttt tccaaggccc ggcggcccgg ataataaaac atgatccaag
    34501 gcttcgccgc gcaatcttgc cgcttcgata aacactttaa gattattgat aattcgctgc
    34561 tgccccacaa attcatcaaa gcgggacgga cggagagaaa tatccaaatc caggtcttcc
    34621 ggtactttac gcggtctcgc taatccgtca tcggtaatgt cttgcatacg ctttacactt
    34681 tatctttttg atcgtattgt tctattattt tttttagagt agcatcgcgc cgcaacgccg
    34741 cacgatgttc gcgtgcagga tatccggaca cggttgtatt ttcgtccact gaaccaatta
    34801 caccggcctg agcagcgatt acagcgtgat cgccgatatt aatgtggcca acaagtccgg
    34861 cctgtccggc gatagttaca tgatcgccga tacgcgtact tcctgcgacg ccggcttgag
    34921 cagcgatcag cgtatgcggc ccgataatca cattatgtcc gatctggata agattatcca
    34981 atttgcatcc acgtccaatt tttgtttcac cgacggttcc gcgatctata caacagttag
    35041 ccccgatctc aacatcatca ccgaccacaa cacgtccaat atgcggaatt ttcaaatatc
    35101 cttcttccgt tttatgaaaa ccaaacccat cgctgccgat cacacttccg gcatgaatga
    35161 tcactcggtc gcctatgatc gtattatcat agatgctgac attgggataa atgacacatc
    35221 cttttccaat acgcacatga tttccgatga cagcatgagc agaaatcaaa gtcccccgac
    35281 ctataatcgt atcataacct atgacggacg atctatgagt ttttacaccg ttatatagtt
    35341 tgctttttat tttaaacgca ttacctctac gaagcgccct ttgatcaatc caccatgtca
    35401 gtacctttat gatatcttca taaggatctt tggataaaat ataagccgtt ttttttttca
    35461 aacattgttc agctaaatct ttagcaaaaa ctttttccga aacaatccaa aattttgaag
    35521 cgccggaaac cgattggggg acataatttt tttgaaacaa aaaaatcact tccgattcca
    35581 aagcgtacat gggttcgttg aatccggtta tttcagaatc cgatccgaca aacggccgtt
    35641 ctaacaattt agcaatgtcg gaaaatttat aacttcgcga cgccatccgc cctcaataaa
    35701 taaaaaatgg aaacctcaat tgaagtttcc attttttttg gatttaaatc aaatttttat
    35761 tttccgcctg ttcctgtggt cggagctgcc gtggccatac gaattaattc atcaatcgtt
    35821 ttttgggtca tgtccaattg aggatcatac gccaaaatca tacccggcga gctgtccaaa
    35881 atcatatcat aacctttagc tttgcgaact gtttcaatcg cttttttcac tttatccaaa
    35941 atcggagcca tcagttcttg ctgtttctta tacatttctt cctgatattt ttgctgcgaa
    36001 ttctggatta ggtatgcgag ttcttccata cctttttgga acgtttgttt gatgttatct
    36061 ttgatcagcg ggttgtcgcg ataatttttc acatagtctt cctgcatcgt ttgcaaggaa
    36121 tcctgcatgt attgaaattg tttataaatg cgttcttgtt cggcttgcaa tttagtttga
    36181 acggtctcag tttctttcag agattgcaaa attttctgag aatcggcata acctatttta
    36241 ccttgtgact ttaccgccgt atttattccc agtgtcaaca tgagaatcgc tatcattgtt
    36301 tttttcacgg atattctcct ttaatatgga aatgtaaatt agttatcttt gattgcagga
    36361 tctgtatcgt tctcttttac aggtttactt gtttcattgg ttgtgctttt agaacttcgc
    36421 cccgaaggcg tacggttggt aggattgctc cctggtgtct ttaaatttgt tcctgttccc
    36481 tgcccctcga cttgagctac tgtcaaacca acggatggta attcttctaa cactagtaag
    36541 gttaaatcta atcgcttgtc aatagacacc ggattacctg tagaaatgtc aaaaacaaca
    36601 tcatattttt ctttcaatcg tacgttgtct atggcgcgtt tcactttgtc taatatgggc
    36661 tgtagtaatt cctgttgttt tttgtataat tcacccgtta caggagaaaa tttctgctca
    36721 cggtaaacta aaatcttttg tttagcatca gcaatattct tctctaacac atttttgccg
    36781 cttttggtca aggtgatact caacgcttca taatcttttt ctattaatgc cacactgtct
    36841 tccatcgccg acagccgatc tgtccaatca cgtgataatt tttccagctc cgtctgcaca
    36901 tcgtgcaccg actgaatctg tgttataaga tattgaaagt ctacataacc aattttacct
    36961 tgcgcgcaca catcgcccat atatacaggg ctaaaggaga taagtaaact caaccaaact
    37021 aagtatttca taaaattccc ttttaactaa aactgctgcc caagctgaaa gtgaagtttg
    37081 gggaagttac ggctcttatc ttttcgatca aggccatacg ctacatccaa accgatcacc
    37141 cctacaagcg gcataaataa gcggaaacca taaccgatag aacgtttgac ctgaaatgga
    37201 tctgtcgtct ttaaagactg aaatacattg cccgcttctg caaatcctaa aataaacatc
    37261 gttggattcg gaataactgg caaacgaatt tcagcggaat attttaccat tgacgttcct
    37321 ccgatcggat acgaaatgcc gctttcagtc accgcttgac cgccgacttt tcttccgccg
    37381 tatccgcgca tttggattga ccctatatcc aacgcaccgc cgcccatgta gaagcgatca
    37441 aattgcggaa tgtcctccac agtcgaagtg gattgaaatg tacgaacata acccatcgta
    37501 aagtccgtat aaaagacaaa attccagaat aacggcatat aattctgagc acggaatgtg
    37561 tttttgaaga aatttttcgg taacacgcgc agcgaattca aatagccgac agtatcaata
    37621 gaaaagaaac cgatatcaga cgtcaatgtg aaaaccgaac cggtttttgg aaattcagcg
    37681 gcatcgcggc tgtcacgatt tattacctgc gataacgtca cagattgagt atggccgtac
    37741 gcaatatagt ctgacacttg actcgtactc aaaggcttaa gttttgaatt accataagca
    37801 acagaccagc tcgccgtaaa aaaatcgtcg ggccacttga ggcgacgacc gaagttaacg
    37861 gagccgccat aactttggct cttatagccg acatactgtg cacggcgatt accatcaatc
    37921 gagtaataca tactggcgcc gaacgaagtg cgagtatcaa ataaataggg ctcctgaaat
    37981 cctatggaaa acactttata gccggcaccg cctaactgta agttgaggtt tacgacctga
    38041 ccgttgccca aaaaattatt gaatgccaca ccggcagaac cgatcaaccc gtcctgagcg
    38101 ctatagccta ttgaaagatt ggctgtatct gtcggcttct cttttaccgt gatgataaca
    38161 tttactttgt cctggctcac cggttgtacg tcaggtataa ccgtttcaaa ataattcaac
    38221 accatcaagt tacgctgcgt acgaatcaac gcatcacgac tgaatttttc acccggatag
    38281 gccaccgttt cacggcgaat tactttttct tttgtcttgg tattatcgcg tatctccaca
    38341 cgctcaatat caacttgatt gccttcagta acaacaaatt cgagattgag tgtatcctta
    38401 ttagatggac gctcaatcgg aacaagttga gcgtaaatat aacccctatc ataatacaag
    38461 gcatttactc gttcctgcat gttacggtca tatttttctt ggtcatacac atcaccgcgt
    38521 tcaaagccca atgctgccgt aagttcgtca cctgaaaaaa gtgtattgcc ggtaaacgaa
    38581 atattaccga tgtgatactt aacaccttct tcgacccaaa tatcaatata caggtccttg
    38641 ttgttctgag cgtaatacac tgaatctttc ataatttgga tatcacgaaa acctctcttc
    38701 ttgtaaaaca tcagcaactt cttaaggtct tcgttatact tcttttcatc aaaatcaccg
    38761 ctcttaaacc atttccaaaa tccgcgcttg gctttagtgt cgtcaaaggc cccttccaat
    38821 ttaccattgc taaaagccgt attcccgtga aaagttattc ctcgaatctt cactttatcg
    38881 ttttcttcaa tttcgatttt aagtaaaacc ttgccggtat ccgtttccga attataagat
    38941 ttcacgtcaa tttcaacatt gcgataccct tcgctcgcat aaagctggcg cacgcgttcg
    39001 gtagcttttt tgatatgttg cgggcttacc gtctgaccat tgaataaacc aaatttggaa
    39061 cgtacatctt tttcttcaat ttcatccgtt ccgtttattt caagattttc caaacgcggg
    39121 tattctttaa cttcaaatac gacggcaatt ccgttttccg tttgttcgcc aaaaacctga
    39181 atatctttga agacattcat ttgccataga ttttccatgg ctttctgaat atcatccaga
    39241 ttaaactctt gatctaccga aagacctgtg gcaataacga tcaattccgg atcggacttg
    39301 atattgcctt cgattttaat ggatgcgatt ttaggcttgg ctccggactg cgcaaacaaa
    39361 gcggatgcac ggttacttcc taagcagagc aaaaaggcgg tgatgacgat gatttttgca
    39421 agtttcatta cgtgattcgt tgtctccgga tattgataca tactgagtta cgtgggtata
    39481 cgataaaatg ttattatttt tctttttttt cttccgcgat ctgctcgctt acttttccaa
    39541 aacgtcgttc gcgtttttga taatccaata tggccttgta cagctgaggt ttgcgaaaat
    39601 ccggccaata aatatccgtt atatacaatt cggcatacgc aatctgccaa agtaaaaagt
    39661 tacttacacg aaattctccg ctcgttcgta ttacaagatc cggatccggc agatcaaagg
    39721 tatccaggta tttagcaaat actgattcgt ccagcgaatc catcaatgat ttttttcgtg
    39781 cagcatcttg ataaagccgt tttacagcgt cgagtatttc aatacgaccg ctataactca
    39841 aagcgagatt gagcaccaag cctgtattat ttttagtaat ttcaatcgct ttttctacct
    39901 gatttagcgt acctgtgggc aacatatcta tgtggccgat tgttttaact cgtacatttt
    39961 gacgattaag gctatcaatc tgattgagaa tcgtacgcat aagcagcttc atcaaggccg
    40021 aaacctccca gcttgggcgt ttccaatttt cctggctgaa agcatacagt gtcagcgctt
    40081 cgatgcccag ttcaccacaa ccctccgtga tatctttgac cgaattaacg ccttcgttgt
    40141 gcccggcaat ccgcggtaat ccacgttgtt tagcccaacg accgttacca tccataataa
    40201 tggctatatg acgaggcata gaccgttttt gcaat
//
LOCUS       DAOQGF010000015        40035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_420_length_40035_cov_7.575713, whole genome shotgun sequence.
ACCESSION   DAOQGF010000015 DAOQGF010000000
VERSION     DAOQGF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 40035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_40035_cov_7.575713"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1484
                     /locus_tag="PK937_03895"
     CDS             327..1484
                     /locus_tag="PK937_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31743.1"
                     /translation="MKTAKWKIVPICLFMVWTMACQEKANEDKGAQYDNDVSLTREAL
                     NEIASILAADPSWITQETITKLSKSSIRLNELPSSSVLSKTSHYNFPVEIELYSKSQA
                     NVMAKGATSTTYYVGLDPDVYHFPEDVQEEETGLPVIEEPAFYYDGNSITETTITIDP
                     KNPTNETIFFIKESNDDQINLAKSASTPIAGTYMAFKKIDLKNKEDASDEEFEVYFSY
                     NDPNSTTIASTTTHIFDGGNHLDAAEVYRYYLDINDKEAYEVDPNEEIALVRVDNLST
                     PLRMVAIEADQSNHTYEQGKYNRNEGANVVVYTQTDFYDMADPTSMVTQNQGYKVDVY
                     NSVNDDRYQHGGIRNITAANLAQRTNDGESSFNTVGVGLTDMDWWMIQLVY"
     gene            1562..2533
                     /locus_tag="PK937_03900"
     CDS             1562..2533
                     /locus_tag="PK937_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31744.1"
                     /translation="MKNLFLVFIQSVVVFSGCIGCLDEHDEKPSLRRKLDKDYEFVIG
                     HVMPDEDRTYPIEVFVGRLLVPRPDTLNNASIQEPLLKIQLRQFQNEYLATLSYHDDA
                     QVFITAGDQTVELQYVGRGTYRDIANALHISNLQTCSLKVIRPKGKTYQAFAKVPGSI
                     SLSSVQGGDTIVSYLKRRKSSQLCFGSPRIIGPQVDGALFYRSQQVDNSHNFDERIVF
                     LEFINSFGCPHTLNNCEEASYANNIWWTSTAFDTVLSHAWGLGETTGARDDMIDTVSY
                     WKYDAPITDRSNITDQKGKKIAGYFGSYNSVSINFVVKAMNDSCTCD"
     gene            2550..3500
                     /locus_tag="PK937_03905"
     CDS             2550..3500
                     /locus_tag="PK937_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31745.1"
                     /translation="MSKLVASIVLLLSSFGCTDHKAKPYEGRLDKDYELVVAHVAPDE
                     DREFPIEVFVGRLHTIFPDSTDAANLQDHMMRIQLRAFVDEYQALLTYHDDAEVYITA
                     GDQTVRLEHVGKGTYRDVADALHVYGQQTCALLVKRSGGKTYQGIVKVPGDITINNIS
                     VGDTVLSYPKKETPQSSICYGETFVSGSVVTDGYVYRFRMPYLYGNEEDIVQLWSSNR
                     AAAPIIISKCGGISYFSIPWTSMALDTNLTRAWGMRETTSIEPAVWDSMRYWRYYAPI
                     TYRSNITDEDGDKIAGYFGAYNSVKTNFVVKVLRDSCECE"
     gene            4040..4645
                     /locus_tag="PK937_03910"
     CDS             4040..4645
                     /locus_tag="PK937_03910"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNH31746.1"
                     /translation="MKNASPSFIFLGGHTVMDFLNTVYLQKNSTVETLQHNKDILVWM
                     EEAGILTHARRNELNRIWIHSPESEAVLRKVHKFRRVCFEVLSCIMAHKPVPKTATDE
                     INFILSSGGMVLRLEESPSGFKKQAVYAFQSPEHFVVPLAVSLVDLLESGDPSRIKQC
                     ANPRCRLFFYDQGKNKTRRWCSMKTCGNRRKSANVYARKRA"
     gene            4705..9105
                     /locus_tag="PK937_03915"
     CDS             4705..9105
                     /locus_tag="PK937_03915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNH31747.1"
                     /translation="MDASIPQNFHPAIARWFVREYRQPTEVQSQAWEAIVEKKHTLIA
                     APTGSGKTLAGFLAIVNDLVIEGLERGLPQETRIVYVSPLKALSNDIEKNLQRPLQAI
                     QDELESLRLPRVEIRVQVRTGDTPAGERTAMLKSPPHILVTTPESLYVLLTSDGGRKM
                     LQSARIAIVDEIHALVGSKRGSHLALSLERLQSLVSRPLTRIGISATQKPLTQVADFL
                     TGRLEPGAAPCTIIDVGHRRAMDLGIEIPRSPLGAVMEGEVWDEIYERLVELISEHRT
                     TLIFVNTRRLAERLSFQLIERLGADVVRAHHGSMSKEHRLDAEQRLKSGSLRALIATA
                     SLELGIDIGSVDLVCQISSPRSIAAFLQRVGRSGHSIHGTPKGRLFPLTRDELVECTA
                     LLDAVHRGELDRILMPEQPIDILAQQIIAETSCRECTEDELFALSQKAYPFRNLKRET
                     FDEILTMLSEGFTTRRGRRGAYLHHDMVHRVVRARPAARLVALTSGGAIPDNFEYDVR
                     LEPTDTFIGTLNEDFAIESSAGDIFQLANHSWKITRVNDGVVRVVDAGQAPPSIPFWL
                     GEAPGRTIELSQAVARLREKIGEQLGSVEMLKPQVLSASVRQNPDGPVVPGKTPWMQP
                     AVQWLCSELGLGLGAAEQLVHYVASGYLALEAMPTQDTIILERFFDEAGDMHLVIHAP
                     FGNRMNRAWGLALRKKFCRKFNFELQAAATDDAIVLSLGSTHSFPIEEVFDYLNSKIA
                     RDTLIQAVFDSPMFEIRWRWNATRSLAMQRTRNGKRVPAQIQRMQAQDLVALVFPDQL
                     ACLENIVGEREIPDHPLVAQTIEDCLYEAMDIETLEDLLRQMENAQIRRIGRDLREPS
                     PFAQEILNARPYAFLDPAPLEERRTRAIANRRWTDPSEAADLGKLDIQAIERVRSEAW
                     PSPENTDELHDALTLIGCMTEQEIRPFQKLADLLIAQKRVAKISSGGSHAVYISVERM
                     PQFKTCGFEVVHPTDLIIPEKILRKTWERDKALVEIVRGRLEGLGPVTTARLAQDLTI
                     AESEVHIALLTLENEGFVFRGSFTDPASAVEWCERRLLARIHRYTMERLRKEIEAVPL
                     SDFMRFLLVWQHVGSADRLEGPEALLTIIEQMEGYEAAAVAWESDILPLRIAEYDPAM
                     LDMACLSGRIVWGRVRYARTSGGSGPVKSTPIVLQQRKHIGLWHAITSADHTGSDALS
                     HAGQAVYDFLKQRGASFFHEVAEYAGVLRSQAEDGMAELVALGLVSADSFNGLRALLV
                     PAKYKTQSAHHRKLPPFSMDNAGRWGLIQTTFTDAIPVAEETALQFSADRLLKRYGVV
                     FRRLVEREAWTPPWRDLVRMLRKMEARGEIRGGRFVESISGEQFALPEAVTKLRAIRR
                     EKPDQAMVALSAVDPLNLLGSVIPGDRVAALAQNRVLYQNGVPIAVLESGAFRTLVKV
                     PENEEWVLREALIRTVVPPRLRPYLGKGIG"
     gene            9157..10293
                     /locus_tag="PK937_03920"
     CDS             9157..10293
                     /locus_tag="PK937_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH31748.1"
                     /translation="MQISLTMVSFLLLISTAHGIFFTILTAGKLRRSSSARILFVIFM
                     LLILANAEYLFIASGAYTTFSHFFGISNGFMLLFGPLMWLYSRSLTDAEFEISKIQSL
                     HCVPYLLFCGVSWPLWFLDHRAKVAFIEVFISGHLPLRVIDYVIFVLQVIHFTAYMLL
                     SLRSVRIEKQQLLDRMYQEDITGRLKWIKFLIGFFCAYLILVSGLVIQVFYSGVFSPD
                     ANYAYTLALSAIMYFAMYYGFNNPDAVTGGFTQKYKNVVLSSEEMSGYTEAIRSAFDD
                     EKMYLQPDLKLADVAKKINASPHLVSQLINQHYGHNFFDHINTLRIAEFKRRLAQPDV
                     NIMTLLGIALESGFNSKSTFNAVFKKFEGITPLEYKKSLNKPQN"
     gene            10384..10899
                     /locus_tag="PK937_03925"
     CDS             10384..10899
                     /locus_tag="PK937_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31749.1"
                     /translation="MKILLIIGAVVVSGLAIYAVYMGVFVSITIREQRIGPYHFIYKE
                     MHGTDAREVGKITTELRLLLTNNGFTNIAPLDIYFPDQKPNHIGFMADDARVEAIITT
                     DSLLKYRNIPEQRCMTTGFPYRNPMSYLVGYIKVDAALSKYRETHGYKKTEAGTIHQD
                     ENILYFQPIIP"
     gene            10996..11514
                     /locus_tag="PK937_03930"
     CDS             10996..11514
                     /locus_tag="PK937_03930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008311419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH31750.1"
                     /translation="MTDHQREHITLRKATSADSIILRFWDEQPHVIESDPNDDWHWEE
                     ELQREPSWREQLIAELDGKPIGFIQIIDPQREETQYWGAIGSGYRAIDIWIGEAGNLS
                     KGYGTAMMHQAIEHCFAEEDVHTVIIDPLANNARAHRFYERLGFRFVREQRFGEDDSF
                     VYELKRGDWMKR"
     gene            11644..14010
                     /locus_tag="PK937_03935"
     CDS             11644..14010
                     /locus_tag="PK937_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31751.1"
                     /translation="MLTNYLKMAIRSFVRNRILSTINIFGLAVGIACCSVIFNYVYDE
                     LTYDGYHVNSEHLYRIEVGNWACTAVGVGPYIKRTFSDVKEITRFYRMPRTIMANGEK
                     RFIEKRGFYADASVFDLFSFDLIQGDPSTALTAPNTIVITTELAHKYFGTEDPVGRSV
                     QIGADADEYKITGVMKAVPAKSHFKFDFLVSLNTLQFPLDNARVQWANTTSYTFALLT
                     ANVNIGTLENQIASAISEKQKAAFGSADPVSVVLQPVYKIHMYSKCEKEIEPSNDINN
                     ILIISSMALFILITAIFNFVNLTTAYFIRRTKEVGLRKTFGADKYQIVRQFLMESILI
                     TLIASVFSLVVMDVLLYFLNSTSNFHLNSSLWSSPLTIVGFLFFSLFVGIIAGLYPSF
                     VLSRLQPAHIIKQNGATSKAFSINVLRKTLLVLQFVISAILIIGTGIIYNQLSFLQNK
                     NLGLEKERVVILPISRISEQNYNTLKFLLGQSSKILGVSGSISIPGERVIYEGIIRQG
                     TVEELGVRLIVADYDFTETYGMTLVEGRNFSNQMSSDSSGAFIINEKAKNLLEYKEVI
                     GKTLEVSGLKQKGAVVGVVKDFNFTSLRSEIQPLVIFLNRSTMRYNYISIKFIPNSTT
                     SAIQQIETVWMKVFPNIPFEYYFLDDAFESLYQSEINLRAIAGIFAILAMVIGALGLL
                     GLVSLSVGQRTKEIGIRKVLGATVFEIVALISKDFLVYVILANLIAWPIIYFGMKRWL
                     DNFAYRIEIDFMIFAEAMLLSLGITIVTVAFQALKAAITPPVETLKCE"
     gene            complement(14071..15981)
                     /gene="acs"
                     /locus_tag="PK937_03940"
     CDS             complement(14071..15981)
                     /gene="acs"
                     /locus_tag="PK937_03940"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNH31752.1"
                     /translation="MSTDNNKPISNQEMYHPSKDVIANAHIKDYETLYRRSVTDREGF
                     WAEEAGKLEWYKSWDRVLDDSKKPFYKWFTGGQTNIILNAIDRHLRDWRRNKLAIIWE
                     GEPGDVRTYSYHALNREVSKFANVLKSMGVRKGEIVTIYMPQIPELVFAMLACAKIGA
                     AHSVVYGGFSVEALAARIEDAKSRVLVTADGGWRRGKINDLKTIADEAIKRSPTIEVC
                     ICVKRTGHEVRMENDRDFWYHDLMSLPIANPKCPTEVMDAEDMLFILYTSGTTGKPKG
                     LVHTHGGYSVYTSTTHRYVFDIKDEDRWWCAADPGWITGHSYIVYAPLINGATVMMYE
                     GAPNHPYPNRWWQIIEKYGISILYTSPTAIRGLMRFGEQWPKRHDLSTLRLLGSVGEP
                     INPEAWKWYYRNIGQERCPIMDTWWQTETGGFMVTPLPVIPLKPGSATKPFFGNEIAV
                     VDDHGKEVAAGEEGKLIIKSPWPGMARTILGDPARFVETYWKEFEKQGWYKAGDSARL
                     DEDGYLWVIGRIDDVIKVSGYRLGTAEIESALVSHPAVSEAAAIGLPHEVKGQAIYSY
                     VILRAGQTPSKELEETLKEHVAKEIGPIARPESVTFLDVLPKTRSGKIMRRVLKARAL
                     GLNEGDVSTLEE"
     gene            16354..17241
                     /locus_tag="PK937_03945"
     CDS             16354..17241
                     /locus_tag="PK937_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31753.1"
                     /translation="MTKKIITLYLSILTLSSCAHQKRVLLHVQGLSNENYQTRSFQIN
                     GSSGVPIETGISVEQGDFVFIEVLGAIHLGSDGGLTNGDGISGYSKQNKYNYKHGSVV
                     TLVAGKDHGMTTISLFRHPIYYQLVDFAKLTDGNYLPCLYFFSDTSGNISFEINDVDY
                     SNNSGEFTVNVYHLKKSGDLGRNSYNKCPMKEPENGKDCEGNTWVKEGIKGWFYHGLF
                     NNSYRGTGKNSGCQCVYTKGDLVVSHPDQGTFDYGFWEYQGDIDDVQARHLHLIWDVL
                     PHDLWIGKKTYNDPHIICK"
     gene            17406..17705
                     /locus_tag="PK937_03950"
     CDS             17406..17705
                     /locus_tag="PK937_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNH31754.1"
                     /translation="MNGKIKYEFSAKTWQHPSPNGWHFVSLPIKMAKEIRENFKRQEE
                     GWGRLKATAKIGNTEWETAIWFDTKHKTYILPLKSEIRKKEKLELNKNIKAMIWV"
     gene            18035..18313
                     /locus_tag="PK937_03955"
     CDS             18035..18313
                     /locus_tag="PK937_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNH31755.1"
                     /translation="MKQKELSKSAEKELSQLTDQELLVEAKKMKSFSINNALFVGFMA
                     GIIIYSVAKNAWGFFTLIPVYFIYKLVNDPKNIRSKVLERLLKERNLK"
     gene            complement(18402..19652)
                     /locus_tag="PK937_03960"
     CDS             complement(18402..19652)
                     /locus_tag="PK937_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNH31756.1"
                     /translation="MISFNGILIILAVILVLWLIDKYVASGLRTLVRDWKKSHIIIRS
                     FWVANVLMYVLVTTGMLFFMLRLGPMPLRATIMGLFVALLVPKLVLVALLLAEDATRW
                     LRKAWSVVRRTKTKEETFLPSRRKFIAQIGFGLAAIPFGSILYGITRGKYDFTVHRTD
                     VFIKDLPSVFDGFTITQLSDIHAGSFDDADAVKRGVDLANAQRSDLLVFTGDMVNNRA
                     EELTPWLNIFKTLNADYGKYATLGNHDYGDYVSWPSPEAKAENLAWLQSMHAEMGFTM
                     LNNAHTVISKNDAHLALVGVENWGLPPWPQKGDLKKALEGIEPAAPKILLSHDPTHFE
                     GEVLQHASRIDLTLSGHTHGMQFGVEIPGFRWSPVQYRYPRWAGLYTENGRALYVNRG
                     FGFIGLPGRVGIWPEITVITLRCG"
     gene            19866..20570
                     /locus_tag="PK937_03965"
     CDS             19866..20570
                     /locus_tag="PK937_03965"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNH31757.1"
                     /translation="MKRLGILLDAVVAIRQMGGGKTPDPLHVISMLEMAGVDSIVYTM
                     MQTGAAEERDLRMLKETIHSHFNLRLVPTMENAQAAIQAKAEMVTMIDRDSTSIRSVN
                     AVEKEALLAPIVSFLRTNGIVVNMLVDTDANQLRTAARMGCDYVELNTDKYTGAASLT
                     LMEQELENMKAMAMAAKKLTLSVTLSGHINHNNIRELLDIEEIEEINIGHSVFARALF
                     VGLDQAVRDFATLIKK"
     gene            20587..21222
                     /locus_tag="PK937_03970"
     CDS             20587..21222
                     /locus_tag="PK937_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNH31758.1"
                     /translation="MHSMTMYDLIERLDEEEEEKETEEPQKEDPLGKKMAEFFLQSRT
                     ILLFEQISPKVTRRVITQLLFLNEQDSKKEIKIFINSPGGVADDGLAIYDLIKLIDAP
                     VKTIVCGLAASAAAIILVAAKKENRLALPHSRIMIHQPLGGVRGSTKDIEISTQQIVR
                     LKQELNEILARETGQPVEKVAQDTNRDYWFTADEAKAYGMISKTVTGLSKI"
     gene            21239..21598
                     /locus_tag="PK937_03975"
     CDS             21239..21598
                     /locus_tag="PK937_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNH31759.1"
                     /translation="MAKFEHHIFICTNTREDGHPRGSCNTGGTNNLQKLFNEALKKYD
                     LKGKVRANKAGCLDQCEHGPNVVIYPDNVWYGFVKDGDVENIVRAMAEGNHASDRILP
                     DSCLNTSACPHRKNGSA"
     gene            complement(21590..22867)
                     /locus_tag="PK937_03980"
     CDS             complement(21590..22867)
                     /locus_tag="PK937_03980"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNH31760.1"
                     /translation="MQDIRTTLTYLYDLQFFGIKFGLENTRALLQFVGNPHKKFPVIH
                     VAGTNGKGSVCAMLASIFEAAGLRCGLYTSPHIVHFSERLRINNIEISENDIVRLTRL
                     LQPEIDRLQCTFFEATTVMAFQYFAEQHVDIAVIETGLGGRLDATNVVEPEVSVITTI
                     DFDHMAYLGDTLEKIASEKAGIIKPDKPVIVGNIPSEAFSVMQKKAHENRSPLYVLGT
                     HITIQNSEVGIDGGCMDAQWRFSDSQGTITKINIPLIGEHQLDNAALAVAAARLQTRF
                     SVSDDDIRKGIRAVIWKGRCFIKRKRPWVIVDAAHNPAGMRSLARSIALLHKEITGQK
                     ILMIGMLSDKDSRNALHEIVPLFDRVVAVTPPNPRALPADALAALIHQTGKPVEVIPD
                     TVSAYDTTLQKLKADDLLVVTGSFYVLQDILLR"
     gene            complement(22918..23304)
                     /locus_tag="PK937_03985"
     CDS             complement(22918..23304)
                     /locus_tag="PK937_03985"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNH31761.1"
                     /translation="MVRTIFRAKIHRARVTQLDLNYHGSITIDQDLLDLVDIWHNEKV
                     QIVNANNGARLETYVIAGKRGSRVIGLNGPAARLAMPGDVISIIAYGAAEESEARAFH
                     PKVVLLNQNNEVVESHTIPSHDDVNR"
     gene            complement(23476..24186)
                     /locus_tag="PK937_03990"
     CDS             complement(23476..24186)
                     /locus_tag="PK937_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31762.1"
                     /translation="MKQRLFFGMMLLATWYNTAFAQSGNPVYGASFYRLWTSSDKISE
                     YAYALRVESSKYGYGVFHWGTRSKDPNESYKGPSSDPFDAAHMTGWFLRPNIWPFGAR
                     KIPVYAHLGYTSRNYNSDVIDEGLNGDLSSHDWSFGLFTKNEYQIKQRVDLAPEIGVY
                     HITRTAKPYGDLSAGDSRGWVYEANLRIGVRLISGLSLITDLGWSDEFSTNGKHSWLQ
                     GEYPGKINAGAGIAYYIR"
     gene            complement(24281..26050)
                     /locus_tag="PK937_03995"
     CDS             complement(24281..26050)
                     /locus_tag="PK937_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNH31763.1"
                     /translation="MNQLVLRLLMLSVVLLGACAPKPPRVEEWLTYYEKSNHLETPRY
                     DETVAYLQRLEQASPWVNLTYFGVSPQGRMNPLVIVDKDRRFNPQAARQSGKNVLLIQ
                     AGIHAGEIDGKDAGMMLIRDLVIFKKNPELLDHTVILFMPFFNVDGHERFGPYNRINQ
                     NGPKEMGWRTTAQNLNLNRDYMKADAPEMRDWLKLFQAWKPDILLDCHVTDGADYRHA
                     VTYAIDQSQNLAPSVRSFTKDVYMPELKNAMAQSGFPMSPYVNLVEERDIAQGLLGGN
                     SPPRFSTGYAALQNRIGFLIETHMFKDYKTRVDGTYNVILHTLETMHRHAPELRQTML
                     DGDASAERMTGSYLPITFRWSKADTIDFLGYNFRMEKSAVTGESFVYWENVPIDYRLP
                     FYDQVSTTDSALVPYAFIVPQQWTSVLERLEAHGIVTEKLDKDRDIKVISYRFSNAKW
                     SENPFEGRLSVNYKTSETEETRRFRAGDRIVKLNQRTARVIMHLLEPRAPDALVQWGF
                     FNAIFEQKEYGETYKLELLARDMLAKDSALRAEFEKRVKEDKNFTGYNRLNWFYERSP
                     WWDKNVNLYPVGKILTAEDISGF"
     gene            complement(26059..26862)
                     /locus_tag="PK937_04000"
     CDS             complement(26059..26862)
                     /locus_tag="PK937_04000"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNH31764.1"
                     /translation="MNTEPHIESHLIVETPENIFLEFELAGLGSRFLAYLIDTAMQAI
                     FFIILVYVLAIAFAFIGGLLHIEEISDGWRNALLAIGIFLLYEGYFIFFETVWRGQTI
                     GKKILRLRVVKDEGYPITFMDAAIRNLMRIADALPTWFYFPSYGLGSAVLIGNPQGKR
                     LGDFAAGTLVIKERTTTGFENFGLLHLNPEMVRGITLANLNRINDDEYFVLRSFFYRK
                     NAFEPMRRMALAKTISAHFRTKLNISQDAWPNDIRLLDTMMLLIEEKKR"
     gene            complement(26872..27513)
                     /locus_tag="PK937_04005"
     CDS             complement(26872..27513)
                     /locus_tag="PK937_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNH31765.1"
                     /translation="MHHSTFSPDREFLVTDEDTLKKLYGTPSEASLAKETDYMHPHYR
                     RFIEVSPFFVLATSGPHGLDASPRGDKPGFVTVENEKTLLIPDRRGNNRMDSLRNIIH
                     NPSVALFFLVPGIGESLRVNGRAQITVDPAWRSRFEMENKLPATVLIVHVEKVFFQCA
                     RAIQRSGLWDPSLHITRDQLPSTGTILEDLSAQRITASEYDRDLYVRQKNTLY"
     gene            complement(27556..28380)
                     /gene="mazG"
                     /locus_tag="PK937_04010"
     CDS             complement(27556..28380)
                     /gene="mazG"
                     /locus_tag="PK937_04010"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNH31766.1"
                     /translation="MESSHETEKIVLDDDASTEAVRTQFGRLYNIMRELRAKCPWDKE
                     QTHASLRQYLLEEAYETLEALDENNYTELRKEMGDLMLQILFHSRIASENNHFDLVDV
                     LRTINDKLIRRHPHVFGDIEAKTADKVLENWEQIKLKEAGRTSVLDGVPKVLPALVRA
                     YRVQEKASRVGFDWGHVDEVWKKVHEEVKELEEAARSGQLQKMEDELGDVLFSVVNLA
                     RFMPINPEDALRGTIEKFTRRFQYVERKYVENQKDMKTATLEEMDQYWDEAKRLGY"
     gene            complement(28389..30683)
                     /locus_tag="PK937_04015"
     CDS             complement(28389..30683)
                     /locus_tag="PK937_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31767.1"
                     /translation="MKSLFFIFVFGLSGLVSAQSLPVTLWLDVQIQEPLASDSNAWTA
                     RALHRSLVPALDKSSYFKIHPSANGAPEYYDGVIRVVCGYEKNELRFQMTVRDTLHSL
                     QKDFSYKTKPGQLRATHEKMMTDIFSSFNIADKKALETVKKDLQNIRSDEAYTMLQQA
                     FAAWSDTRTADSWIQKALQKEPRASLLYERAGELAAARNEYDAAISYYQKTLELDSAR
                     FYIYRKIADVHYDHKNNLKSAKANYFEATHRDPTDAASWLQLGYCAYLEKDYTIARNY
                     ANKAVEVWQKKFGKTVYRETATAWNLIGNVAVAERDTAGAREYFEKATNTNPYDIMAR
                     KNLARLYEFDRRWDKAVSMYGQVLTLESQDITARLSLANLMYQKKRYVDAARYYVSAV
                     MIKPESENQRENPIQILQWMSANRKDIKPLQTLMDSVDERILDDATDPKKQFELRITS
                     GYVYLYYLQNPTEAIHEFKSAQSLNSRAYRLDYYLGEAYFQVGSYDLALPFLRRYAEH
                     AEDSYTYAKVFLLLAKTLNKLKRYEEAQIEIIKSIRVYPNAESYYVQGIAFRGTQNNE
                     EAVRAFERAVKVFPNYTQAHYELGRAHMALRKYDKALPELQKAVELDSSNYPYRQTLA
                     RWYSTLERWEEADASLAATERRMLSAGVKDPTVYEDWGEQYFQQKKYDVAAQKYTLAY
                     GADTTTIQACFRLASIAASQHDGTSALRWLSRAFRNRFVDFNALLKDKNFNALRQTPE
                     YVELVGAYERAYNEEVQKKINLTR"
     gene            complement(30680..31681)
                     /locus_tag="PK937_04020"
     CDS             complement(30680..31681)
                     /locus_tag="PK937_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH31768.1"
                     /translation="MPGTVRIEVLTSEVLQNNPLNDPHQRKLAVYLPPSYTTAPSRRF
                     PVIYLLSGFMGYGTMMLSPQAWGYAIDERCDKLIAEHAMEECIVVMPDCFTRWGGAQY
                     MNSPATGNYEDYIIREIIPYIDRTFRTQTERNHRGIMGKSSGGFGALRLAVNYPELFS
                     AFFCSAGDMYFEYGYKPDIPKTYNALYKTGGLDGFLKKFFEAPKKSGEQITAINIIAM
                     AAAYSPQASEPYGIELPFDLQTGALKESVWQRWIAHDPVFCVDQKAWRENLSKINHIF
                     LDCGARDEYHLHIGARIFADKLRNYSIPHWYEEFDDGHMGTSYRYDVSLSKLSRSIA"
     gene            complement(31960..32301)
                     /locus_tag="PK937_04025"
     CDS             complement(31960..32301)
                     /locus_tag="PK937_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNH31769.1"
                     /translation="MPRVTKKNTPKRAIPKRFQRFLKEYPEVGKAYSALSHAVQDAGP
                     LDEKTRTLIKIGISAGSRQEGALHSHVRKALDAGATPEEIRHTILQCLPTIGFPGMMA
                     AMSWADDILNG"
     gene            complement(32306..33601)
                     /gene="nrfD"
                     /locus_tag="PK937_04030"
     CDS             complement(32306..33601)
                     /gene="nrfD"
                     /locus_tag="PK937_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNH31770.1"
                     /translation="MEHLVTPDLPHQAGKITDDIRPVIARSFIRDAFDSITRGRPGYH
                     IWMGILTFFMLAGAYAYSVQLSEGLAVTGMNDRVSWGFYISNFTFLVGVAAAAVMLVL
                     PTYILKDVDFSKAVLIGEGLAVSALIMCLAFVTVDLGGPGRAWHLIPGIGRFNWPSSM
                     LTWDVLVLNGYLFLNLTIPFYILMCHYQGKHPNPKVYVPGVIISVFWAFCIHLVTAFL
                     YAGLPARPFWNSSLLGPRFLASAFAAGPALIIIVLSVIRRYTAYKVENVTLGKIALIV
                     TVAAQINLIMLGSELFKEFYKPTHHSESAVYLFFGLHGFGALKPWIWTAIGLNLIATA
                     ILTMNPLRSNHKWLLPACALLFLSIWIEKGMGLIIPGFIPGPWGKIVEYAPTWVEIFV
                     ALGIWAMGMFIFTLLVKIAIPIETGVLRYQPAKPSQTKN"
     gene            complement(33605..34744)
                     /locus_tag="PK937_04035"
     CDS             complement(33605..34744)
                     /locus_tag="PK937_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH31771.1"
                     /translation="MSHCQCQHTPDPRREGLRDLLAENVLSDWLKRDTEWDYLRATPS
                     LENVMRWSQYRDDHTSVRPDPKATAADHVLEQSMDRRSALQNMAAAFVAGLGLLQTAC
                     NPLAPAEDREKAQLAWDDYFQGNFRMMSDAEKREAVSRLERMYEMHTGKHAHISDQGP
                     IPGVLYGYAFNISKCRGYLECIRGCIKENNQDRRSGMQYITVHEHKKGRMDFSDAPDN
                     FYHEVPMEGHFYIGTQCMHCENPPCVDVCPVQATWKEQDGIVVVDYNWCIGCRYCAAA
                     CPYDARRFNWQDPVVPENEVNLNQHYLGNRLRKKGVMEKCTFCIQRTRNGKNPACVEA
                     CPTGARIFGNLLDPSSEIRWVLANKKVFRLKEDLGTEPKFWYFMD"
     gene            complement(34763..35416)
                     /locus_tag="PK937_04040"
     CDS             complement(34763..35416)
                     /locus_tag="PK937_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH31772.1"
                     /translation="MRQHMLFSANTRNFKILRTMAGVCLAVFFVSCGHDHAQGLLEKL
                     DTAPAWSDSAYTHVAPTADTASLHAVQLVERPLYVVPRTPLIKKYPCTECHTKPLGQM
                     KSSDPSGRKAHWDKNLQHATTNVLQCTVCHDERNLNNLRSITGAVIAFNDSYQLCQQC
                     HSRQFNDWQGGAHGKRVGGWAPPRTVALCVECHNPHQPRWDIRWPAQPSLTMQRLIK"
     gene            complement(35436..36134)
                     /locus_tag="PK937_04045"
     CDS             complement(35436..36134)
                     /locus_tag="PK937_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNH31773.1"
                     /translation="MNLKKLLLPENRVIFISLSALLMLCVIVIVGQSVIQSGAEAYTP
                     SAKTTGEAVLPDEAGVFKQSDQALYYLETLHQQGSRTLAAFYERRAFDGAPPVIPHEV
                     TDERSVGGNTCLQCHENGGFVPKFNAYAPVSPHPQFLSCRQCHVPVTTRNVFRASGFT
                     PVKPPAINRSALTGAPPQIPHDLQMRENCLACHGGPASVREIRTPHPDRVNCRQCHGA
                     IEDNTAWTRPAPTP"
     gene            complement(36131..38443)
                     /locus_tag="PK937_04050"
     CDS             complement(36131..38443)
                     /locus_tag="PK937_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH31774.1"
                     /translation="MNRRELLKHMATVAAMSAAASIFPGIVFSGEQGIERFLPDEKLD
                     WKKSPCRFCGTGCGLLIGIQNGKAVAVKGDPASAVNRGLCCIKGYHSVMALYAKDRLT
                     KPLIRKNGQLVETTMKEALDLIAQKMKECIDKNGKDSVAIYGSGQWTVPDGYVASKFM
                     KGGIGTNNLECNARLCMASAVTGFMTTFGQDEPMGCYEDIDHADVYILWGNNMAEMHP
                     VLFSRMLDNRLRRGAKIIDFATRTTRTSMAADKSILFKPQTDLAVANAIAYELIRTGR
                     INQNFIQKHTTFKKGKTQIGYGLEDKFAFQDEPQGASLEEYKKFLEDYKPEIVEKVSG
                     VSASDIRYLASFYGDANKKVTSFWCMGMNQHTRGTWIQNLVYNIHLLTGKISTPGNSP
                     FSLTGQPSACGTAREVGCFTHRLPHGEVTNEKDRQHAAEIWKVPVEKIPAKPTYHTVE
                     MFRALDRGDIRFMWIQVTNPMVTMPKLKRYRDGAKKEDRFIVVSDVYPTPTTDIADVI
                     LPSAMWIEREGMFGNSERRTQHFEQMLQPPGECIADAWAMIEVARRLGHGALFPWDSK
                     NYVEEIWKEYTQFHHGKDHEMATYEMLKANPGVLWPCPEGKETKWRYHAAYDPAAKGD
                     AWDFYGKPEGKAWIWIRPYEPPPEMPDDDYPYWLNTGRVLEHWHSGSMTRRIPVLHQA
                     VPSSYVELNPDDAKELGIRHGDKVRLTSRRGSIVMSASINERGVPPHGMVFVPFFDES
                     HLINELTLDAFCPISRQPDFKKCAVKVERA"
     gene            complement(38440..38706)
                     /locus_tag="PK937_04055"
     CDS             complement(38440..38706)
                     /locus_tag="PK937_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31775.1"
                     /translation="MPVKSYLAYPMQDQREALAKALKELPNCEIVPSDNYDVFVIVTD
                     TSDDAQEKEIEEKLLKIESIRFLSLVSGYEDRTTTNTNMQGGLA"
     gene            39226..39552
                     /locus_tag="PK937_04060"
     CDS             39226..39552
                     /locus_tag="PK937_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH31776.1"
                     /translation="MKKTKLGEFEELVLLTVIALRKDAYGVEIKRELEERLNETLSVG
                     SIQSALKRMEEKGYLTSAFGEPTQKRGGKRKRIYTATPAAHRVLSELKSIRAGLWNSM
                     PRLVHT"
     gene            39560..>40035
                     /locus_tag="PK937_04065"
     CDS             39560..>40035
                     /locus_tag="PK937_04065"
                     /inference="COORDINATES: protein motif:HMM:NF038404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 2-containing transporter"
                     /protein_id="HNH31777.1"
                     /translation="MHTPDSIQPPRWPIAVLRFLLKKEYLEEIEGDLEEHFADDIEQM
                     SVFKARMYFIWNALTLVRPSLLRHIGSFHIQHHPAMFRNYFKISFRSLMRQPFQSFIN
                     IFGLSAAIGICIFTYAFSRWTYSMDQFHTHKNEVYLTTFYANRDGSDQQFGRSPAPL"
BASE COUNT        10595 a         8993 c         9634 g        10813 t
ORIGIN      
        1 atctaagcca tctaagccat ctaagccatc taagccatct aagccatcta agccatctaa
       61 gctcgatgat tgagttgaat tgaagttggt atgactttga aattccaaaa aaaaggaggt
      121 gagggcattc gcaatttaca aagatagcag tcggtccaat taattttttc acaaccgaaa
      181 caaaaaagga agatgcatat taccaaattc agcgcttggg gcattgaatc ataaagtaat
      241 atgatcttcc cgttgccaat ctaatatatc acaaatggat tagcaatcat ttgtttcccc
      301 aaatttttct aaagggagtc ttacaaatga aaacagcaaa atggaaaatc gtcccgatct
      361 gcctttttat ggtatggacg atggcgtgtc aggaaaaagc gaatgaagat aaaggcgctc
      421 aatatgacaa cgatgtttcg cttacgcgtg aagcgctcaa tgaaatagct agcattttag
      481 ctgccgatcc ttcatggatt acgcaagaaa ctattacaaa attaagtaaa agtagtattc
      541 gactcaatga gttgccttcc agttccgttc tttcaaaaac aagtcattat aactttccag
      601 ttgagattga gttgtattcc aagtctcaag cgaatgtgat ggctaaggga gctacttcaa
      661 caacgtatta tgttgggctt gatcccgatg tctatcattt tcccgaagat gtacaggagg
      721 aagaaacagg gttgcctgta atagaagaac cagcctttta ttatgatgga aactcgataa
      781 cggaaactac tatcaccata gatcctaaga atccaacaaa tgaaacaatt ttctttatta
      841 aagaaagtaa tgatgatcaa atcaatctgg caaaatcagc ctccacgcct attgctggga
      901 catatatggc cttcaagaaa attgacttaa aaaacaaaga ggatgcaagt gatgaagagt
      961 ttgaagtgta cttttcatat aatgatccca attcaacgac aatagctagc actacgacgc
     1021 atatttttga tggaggcaat catttagatg cggctgaagt atataggtat tatctggaca
     1081 ttaatgacaa agaagcatac gaagtggatc cgaatgaaga gatagctctt gttagagttg
     1141 ataacctttc aacaccactt cgcatggtgg caatcgaagc agatcaatcc aaccatacat
     1201 acgaacaagg gaaatataat agaaacgaag gcgccaatgt tgtggtctac acacaaacag
     1261 atttttatga tatggctgat ccgacatcga tggtcacaca aaatcaaggg tacaaagtag
     1321 atgtgtataa ttcggtcaat gatgaccgct atcagcacgg ggggattaga aatatcactg
     1381 cagctaactt agcgcaaaga actaacgatg gtgaaagttc atttaatacc gtcggtgtag
     1441 gtcttaccga tatggattgg tggatgattc aattggtata ttaagattca ttgaaagatc
     1501 attctaagta tgggctatct ggaagtaata ttcgggtagc ccactaattt tggacaacaa
     1561 aatgaaaaat ctttttttag tttttattca gagcgttgtt gttttctcag ggtgcattgg
     1621 ctgcctcgac gaacacgacg aaaaaccatc attacgccga aaacttgaca aagattatga
     1681 gtttgtgatc ggccatgtta tgcccgacga agatcgaacc tatccgatcg aagtttttgt
     1741 gggtcgccta ctagtgcctc gccctgatac tttgaataac gccagtattc aggaaccact
     1801 gctcaagatt cagcttcggc agtttcaaaa cgaatacttg gcaaccttat cctatcatga
     1861 tgatgctcag gtttttatca cggcaggcga tcaaaccgtg gagcttcaat atgtaggtag
     1921 aggtacatat cgggacatcg ccaatgcact ccatatctct aaccttcaaa cttgttctct
     1981 caaagttatt cggcctaagg gcaaaactta ccaggcattc gctaaagttc caggtagtat
     2041 atcactcagc agtgtgcaag gcggagatac aatagtatca tatttaaaaa gaagaaaatc
     2101 atctcaactt tgttttggtt caccaagaat cattggacct caagtggatg gagctttatt
     2161 ttatcgttcg caacaagttg ataatagtca caactttgac gaacgcatag tcttcttgga
     2221 atttattaat tcctttggct gtccacatac acttaataat tgcgaagaag catcatatgc
     2281 aaataacatt tggtggactt ctacggcttt tgatacagtg ctatcccatg cctggggcct
     2341 cggtgaaaca accggcgcac gtgatgatat gattgatacg gtgagttact ggaagtatga
     2401 cgctcctata acagatcggt cgaatattac cgatcaaaaa ggaaaaaaaa ttgcaggata
     2461 ttttgggtca tataattcag taagtattaa ctttgtcgta aaggcaatga atgattcttg
     2521 tacatgcgat taagctagat aaggcatcca tgtcgaagct agtagcctct attgtattgc
     2581 ttttaagtag cttcggttgt acggatcata aggcaaaacc ctacgaaggg cgccttgata
     2641 aggactatga attggtcgtg gcgcatgtag cgccggatga agatcgtgaa tttccgatag
     2701 aggttttcgt cggacgactc cacactattt ttccggactc gacggacgct gccaatttgc
     2761 aggatcatat gatgcgaata caactgcgcg cgtttgtgga tgagtatcaa gctctgctta
     2821 cctaccatga cgatgcggag gtctatatca cggcaggcga tcagactgtc cggttggagc
     2881 atgtcggcaa aggcacctac cgggatgtgg cggacgcgct tcacgtttac ggtcaacaga
     2941 cttgtgcact gcttgttaaa agatcgggag ggaaaacgta tcagggaatt gtaaaagtac
     3001 ccggtgatat tacgatcaat aatatatccg taggtgacac agttctatct tatcccaaaa
     3061 aagaaactcc tcaatcaagc atatgttatg gggaaacgtt tgtcagtggt agtgtagtta
     3121 cggatggtta tgtctatcgc tttcgtatgc catatcttta tggtaacgaa gaggatatag
     3181 tgcaactatg gtcatcaaac cgtgcagccg ctcctatcat aattagtaag tgcggaggaa
     3241 tttcatactt cagtattccc tggacatcca tggcgctgga caccaatctt acgcgggcat
     3301 ggggtatgcg agaaacgaca agtattgagc cggcggtttg ggattctatg aggtattggc
     3361 gttattatgc acctattacg tatcgttcga atatcacgga tgaggacggc gataagatag
     3421 ccggttattt cggtgcatat aattcggtga agacaaactt tgtagtgaaa gttttgaggg
     3481 attcttgtga atgcgaatga gaaagtgaag aaaataaaat cgccattgcg gctgtcttgt
     3541 aaggtttgat tcagattgct ttctataaat ctcattttag atatatacac acgatgttaa
     3601 ttctattttc tctttggtgc ctatagagta tttgttgttt aaaaatgaag gattaattaa
     3661 ctaaaacaat tttctttttt acaggactgt aagtatgcgc cccaacctca agcttattct
     3721 aaattatcgt aaaacacgat aaagatcacg tcgattaata tttccccaaa tagtatttca
     3781 tattagactg taatatcacg agcgaagcgt agtaaaatgc cttgtacaat aggaacgcac
     3841 tgcttaaact gatcaatgaa caactatgtt taaaatgcat agttgataac cgattacatt
     3901 aggttcggtt tcatgctacg attaattggt ggtactcgat tgttttcgtc catagttttt
     3961 tcttaacgta aactttgaca tacactcgta tcaaactgcg aaaattggct atctgatcac
     4021 atattttcaa agatcacaaa tgaaaaacgc atctccatct tttatttttt taggcggcca
     4081 taccgtaatg gattttctca atacggtgta tcttcagaaa aacagcactg tagaaacatt
     4141 gcagcataac aaagatattt tggtatggat ggaggaggcg ggtatactca cgcatgcgcg
     4201 tcgtaatgaa ctgaatcgaa tatggatcca ttcacccgaa tccgaagccg tgttgcgaaa
     4261 agttcataaa tttcggcggg tatgttttga agtgttaagc tgtatcatgg cacataaacc
     4321 tgtaccgaaa acggccacgg atgagataaa ttttattttg tcatccggag gcatggtgtt
     4381 acggcttgag gaatcgccgt cgggttttaa aaaacaagcc gtgtacgcat tccaatcgcc
     4441 ggagcatttt gtagtacccc tagccgtttc gttggttgat ctcttggagt ccggcgatcc
     4501 gtcgcgcata aaacagtgtg cgaatccccg atgccgtttg tttttttacg accaaggaaa
     4561 aaacaaaacg cggcggtggt gcagtatgaa aacctgcggt aaccgcagga aatcggctaa
     4621 tgtttatgcg cgaaagcgcg cttaacatgt tttccgttaa cgaccgtttg caaacctcca
     4681 tgtagaaatg gcattgtatt ttttatggac gcttcgatcc cgcaaaattt tcatccggct
     4741 attgcacgct ggtttgtccg tgaatacagg caaccgaccg aagttcaatc acaggcatgg
     4801 gaggccattg tagaaaaaaa gcatacgctg atcgccgcac ccacgggttc cggtaaaacc
     4861 ttggccgggt ttcttgcaat tgtaaatgac ttagtcatcg aaggtcttga acgcgggctt
     4921 ccgcaagaaa cgcgcattgt gtacgtatct ccgctcaaag cgctttccaa cgatatcgaa
     4981 aaaaatcttc aacgcccttt gcaggctata caggatgaat tggaatcact gcgattgccc
     5041 cgcgtagaga tacgcgtgca agtgcgtact ggggatacac cggcggggga gcgtaccgct
     5101 atgctcaagt cgccgccgca tattctcgtc actacgccgg agtcgttata cgttttgctc
     5161 accagcgacg gtggtcgcaa gatgctccaa tccgcgcgta tcgctatcgt ggatgaaatc
     5221 cacgccttgg tcggcagtaa acgcggatcg catctcgcct tgtcgctcga acgcctgcaa
     5281 tcgctggttt cccgaccgtt gacccggata ggcatctccg cgacgcaaaa accacttacc
     5341 caagttgccg attttctcac cggccgcttg gagcccggtg ctgcgccgtg tacgatcatt
     5401 gacgttggtc atcgacgtgc gatggacctc ggtatcgaaa taccgcgttc gccgctcggc
     5461 gccgtgatgg aaggcgaagt gtgggacgaa atctacgagc gcttggtcga actgatttcc
     5521 gaacaccgca cgacgcttat ttttgtcaat acgcgccgat tggcagagcg gctttcgttt
     5581 caactcatcg aacgtctcgg tgcggatgtc gtacgcgcgc atcacggcag tatgtccaaa
     5641 gaacatcgcc ttgacgcgga gcagcgactc aaatcgggat cgctccgcgc tttgatcgct
     5701 acggcttcgc tggagctggg tattgacatt ggctccgttg atctcgtatg ccagatcagt
     5761 tcaccgcgtt cgattgcggc gtttctgcag cgtgtaggcc gttcgggtca cagcattcat
     5821 ggcacgccca aaggacgctt gtttccgctc acgcgggacg aacttgtcga atgtacggct
     5881 ttgctggacg ccgtgcaccg aggagaactg gaccggattc tgatgcccga acaaccgatt
     5941 gacattttgg ctcagcaaat catcgccgaa acatcctgcc gcgaatgcac cgaagatgag
     6001 ttattcgctc tgagtcaaaa ggcttatccc tttcgtaacc tgaagcgcga aacgtttgac
     6061 gaaatactca ccatgctttc ggaaggattt acgacgcgcc gcggtcgccg gggtgcttat
     6121 ctccaccatg atatggtcca tcgtgtcgtg cgtgcacgtc ctgccgcacg gctcgtcgct
     6181 ttgacatccg gtggagccat cccggataat tttgagtacg acgtccgtct cgaaccgacg
     6241 gatacgttta tcggtacgct caacgaagac tttgccatcg aaagttcagc cggcgatatt
     6301 ttccagttag ccaatcattc ctggaaaatc acccgcgtca atgacggtgt cgtgcgcgtc
     6361 gtggatgccg gacaggcgcc gccttcgatt ccgttttggc tcggcgaagc gccggggcgc
     6421 acaatcgaat tatcccaagc ggtcgcacgc ttacgcgaaa aaatcggtga acagttgggt
     6481 tcggtcgaaa tgcttaagcc gcaggtgctc agtgcgtcgg ttcggcaaaa tcccgacggt
     6541 ccggtcgtac cgggaaaaac accgtggatg caaccggccg tgcaatggct ttgttcggaa
     6601 ctcgggttgg gattaggcgc ggcggaacaa ctggtgcatt acgtcgcttc ggggtatttg
     6661 gctttggaag cgatgcccac gcaggatacg atcatcctcg aacgtttttt tgatgaagcg
     6721 ggcgacatgc atttggtcat tcacgctccg tttggcaatc ggatgaatcg tgcgtgggga
     6781 ttggcgttgc gcaaaaaatt ttgccgtaaa tttaattttg aattgcaggc ggctgccacc
     6841 gatgatgcca tcgtattatc cctcggttcg acgcacagtt ttcctattga agaagtattt
     6901 gactacctca attccaaaat tgcgcgggac acgctgatcc aggccgtatt cgattcgccg
     6961 atgtttgaaa tccgttggcg atggaatgcc acgcgttcgc tggcgatgca gcgcacccgc
     7021 aacggtaaac gcgtaccggc gcaaatacag cgcatgcaag cgcaggatct cgtcgcattg
     7081 gtatttcccg atcagttggc gtgtttggaa aacatcgtcg gagaacgcga aatcccggat
     7141 catcctttgg tcgctcagac gatcgaagat tgtttgtatg aagcgatgga tatcgaaacg
     7201 ctggaagatt tgcttcggca aatggagaat gcacaaatcc gtcgcatagg tcgtgatctg
     7261 agggaaccgt cgccgtttgc gcaagaaata ctcaacgccc ggccctatgc ctttttggat
     7321 ccggcaccgc tggaagaacg ccgcacgcgt gcaattgcca atcgtcgctg gaccgatcct
     7381 tctgaagcgg ccgacctcgg taaactagat atccaggcta tcgaacgcgt acgcagtgaa
     7441 gcgtggccgt cgccggaaaa tacggacgaa ctgcacgatg cgctgacgct gatcggatgt
     7501 atgacggaac aggagatacg tccgttccaa aaattagccg acctgctgat cgctcaaaaa
     7561 cgtgtcgcta aaatttcatc cggcggatcg catgccgtat atattagtgt cgaacgtatg
     7621 cctcagttta aaacctgcgg gtttgaagtg gtgcatccga ccgaccttat cattcctgaa
     7681 aaaatacttc gcaaaacctg ggaacgtgac aaagcgctcg tcgagatcgt acgcggtcgc
     7741 ctcgaaggtt tgggtccggt cacaaccgcg cgcctcgcgc aggatctgac catcgccgag
     7801 tcggaagtgc acatcgcgct gctaacattg gaaaacgaag gttttgtatt tcgcggttct
     7861 tttaccgatc ccgcatcggc ggtcgaatgg tgtgaacggc gtttgctggc gcgcattcat
     7921 cgctatacga tggagcgact gcgtaaagaa atcgaagccg tgccgctgtc ggattttatg
     7981 cgttttcttt tagtgtggca gcacgtcgga tcggcggatc gcttagaagg gccggaggct
     8041 ttgcttacga tcatcgaaca aatggaaggc tacgaagcgg cggcggtagc gtgggaaagc
     8101 gatatccttc cgctgcgtat cgcagagtac gatccggcca tgttggatat ggcgtgtctg
     8161 tccgggcgca tcgtgtgggg gcgtgtgcgg tatgcgcgca cctccggcgg gagcgggccg
     8221 gtcaagtcca caccgatcgt gctgcagcag cgcaaacata tcggattgtg gcacgcgatc
     8281 acatccgcag atcatacggg cagcgatgcg ttatcccatg cggggcaggc ggtgtatgat
     8341 tttctcaagc aacgcggcgc gtcatttttt catgaagtgg cggagtacgc gggcgtactg
     8401 cgttcgcagg cggaggacgg tatggcagag ctggtcgcgc tcgggctggt gagcgccgac
     8461 agtttcaacg gattgcgggc gcttttagta ccggccaaat ataaaacgca atcggcacat
     8521 catcgtaagc tgccgccttt ttcgatggac aatgcgggcc gttggggctt gatccaaacg
     8581 acatttacgg atgcgatacc ggttgccgaa gaaaccgcgt tacagttttc agccgatcgg
     8641 ctactcaaac gctacggggt tgtgtttcgc cgattggtcg agcgcgaagc gtggacgccg
     8701 ccgtggcggg acctcgtgcg catgctgcgc aaaatggaag cgcgcggaga aatccgcggc
     8761 ggacgttttg tggagagtat ttccggtgaa cagtttgcac tgcccgaagc ggtgaccaaa
     8821 ctgcgtgcga tccgtcgtga aaaaccggat caggcgatgg tcgcgctctc ggccgtagat
     8881 ccgctcaatc ttttggggag cgtcatcccg ggcgatagag tggccgcgct cgcacaaaac
     8941 cgtgtgctgt atcaaaacgg tgtgcccatc gctgtattgg aaagcggcgc atttcgtacg
     9001 ctcgtcaaag tacccgaaaa tgaggaatgg gtgctgcgcg aagcgctgat tcgtaccgtc
     9061 gtaccgccca gactgcgccc ctatctgggc aaaggcatcg ggtaactgaa atacaaatat
     9121 tgtttcgcaa tgtataacca acttatgtgc tgacgtatgc aaatttcgct cactatggta
     9181 agttttttac ttttgatcag cacggcacat ggtatttttt ttacgatact gacggcgggc
     9241 aagttgcggc gctctagctc ggcgcgtatt ctttttgtca tattcatgct gttgatactg
     9301 gcgaatgccg aatatctttt tatcgcttca ggcgcgtaca cgacattttc tcatttcttt
     9361 ggcatttcaa acggattcat gcttcttttt gggccactga tgtggttata cagccgcagc
     9421 ctcacggatg cggagttcga gatatcaaaa atccagtcgc ttcattgcgt gccttattta
     9481 ttgttttgcg gagtatcatg gccgctttgg tttttagatc accgggccaa agttgcattt
     9541 atcgaggttt ttatttcggg ccacttgccg ttacgggtca tagactacgt gatcttcgtt
     9601 ttacaggtga ttcattttac ggcgtatatg cttttgtctt tgcgttctgt tcgcatagaa
     9661 aagcaacaac tgcttgatcg catgtatcag gaggatatta ccggacgatt aaaatggatt
     9721 aaattcctca tcggcttttt ttgcgcgtat ctgatactgg tttcggggtt ggtgatacag
     9781 gtgttctata gcggggtgtt ttcgccggat gctaattatg cttacacgtt ggctttatcg
     9841 gcgatcatgt attttgcgat gtattacgga tttaataatc ccgatgcggt taccggtgga
     9901 tttacccaaa aatacaaaaa cgtcgttcta agttcagagg aaatgtccgg atatacggaa
     9961 gctattcgca gcgcatttga tgatgaaaaa atgtatttgc aacccgattt aaaattagcc
    10021 gatgttgcca aaaaaataaa tgcctcgccg catcttgtat cgcagctgat caatcagcac
    10081 tacgggcata atttttttga tcatatcaat acactacgta tcgctgagtt caaacgtcgt
    10141 ttggcacaac cggatgtaaa cataatgaca ttgttaggca tagccctgga atccggtttc
    10201 aacagtaaat ctacattcaa tgccgtattc aaaaaatttg aaggaattac cccgctagag
    10261 tataaaaaaa gcctgaataa acctcaaaat tgagtccgac tttataaagt tgagcgattc
    10321 ccgccgataa agagattatt ttacccgatc attttctttt ttaatcaaac attaaagggc
    10381 gttatgaaga ttttactcat tatcggcgcg gttgtcgtat cgggattggc gatttatgca
    10441 gtttatatgg gcgtttttgt atccattacc atacgtgagc agcgtattgg tccgtatcat
    10501 tttatttaca aagagatgca cggaacggat gccagagagg tcggaaagat aacgaccgaa
    10561 ttacggcttt tattgaccaa taatggtttt acaaatattg caccgctcga tatttatttt
    10621 cccgaccaga agcccaatca cattggtttc atggccgacg atgcgcgcgt ggaggcgatt
    10681 atcaccacag actcgctttt aaaataccga aatattccgg aacagcgctg tatgacgacc
    10741 ggttttcctt accgaaatcc gatgtcgtat ttggtcggat acatcaaagt ggatgccgct
    10801 ttgagcaaat accgtgaaac ccacggttac aaaaaaacag aggcgggtac catccatcag
    10861 gatgaaaaca tactgtattt ccaaccgata attccgtaac ttcacatatc cgatttggtt
    10921 tattttttaa ttcaggtgtg gttgctgcac agtcagcgtc cacgccattc gttatttact
    10981 ttatcgtcct tagctatgac ggatcatcaa cgagaacaca tcacgctgag aaaagcgact
    11041 tctgcggatt cgattattct ccggttttgg gatgaacagc cgcacgtcat agagtccgat
    11101 cccaacgacg attggcactg ggaagaggaa ctgcagcgcg aaccgtcgtg gcgggaacag
    11161 ctcatcgccg agcttgacgg gaaacctatc ggctttatac agatcattga tccgcaacgg
    11221 gaagaaaccc aatactgggg tgcgatcggg agtggctatc gtgcgattga tatatggatc
    11281 ggtgaagccg gaaatctgag caaaggttat ggaactgcga tgatgcacca ggccatcgaa
    11341 cactgttttg cagaagagga tgtgcatacc gtgatcatag acccgctggc gaataacgcc
    11401 cgggcgcacc ggttttacga acgtctggga tttcgttttg tacgcgaaca acgattcgga
    11461 gaagatgaca gctttgtgta tgaactgaag cggggagatt ggatgaaacg atagggtggt
    11521 ggttatttaa agccgtgatc ttctatttaa atttgaacct gatggcgata tcaagaaata
    11581 ggtggcgttt tgtaaagcaa tagtgtcgtt tctagatgtg ctttaaccga tgtgaggaaa
    11641 atcatgctga caaactacct taaaatggct atccgttctt ttgtgagaaa tagaattctc
    11701 tcaacgatta atatctttgg attagccgtt ggtattgcat gttgctcggt aatttttaac
    11761 tatgtttatg acgaattaac gtacgatggt tatcatgtta attcggaaca tctttatcgt
    11821 attgaggttg gtaattgggc ttgtaccgcg gtgggggttg gcccttatat caaacgaact
    11881 ttttctgacg taaaagagat cacgagattc taccgaatgc cacgaacgat aatggctaac
    11941 ggagaaaaac gatttattga aaagagagga ttttatgctg atgcctcggt ttttgatttg
    12001 tttagttttg atctaatcca aggagatccg tcaaccgcgt taacagcacc caatacgatc
    12061 gtcattacga ctgagttggc tcacaaatat tttggaacag aagatccggt gggtcggagt
    12121 gtacagatag gcgccgatgc cgatgaatat aaaatcacgg gagttatgaa agcggttccg
    12181 gcaaaatctc attttaagtt tgatttttta gtttcactca atactctcca atttccattg
    12241 gataatgctc gtgtgcaatg ggctaatacg acgtcatata cttttgcact acttacagca
    12301 aacgtaaata tcggtacact tgaaaatcag attgcatcag caatatcgga aaagcaaaag
    12361 gctgcgtttg gatctgctga cccggtttca gttgtgctgc aaccggttta caagattcat
    12421 atgtattcca agtgcgaaaa agaaatcgaa ccaagcaatg atatcaacaa tatcctcata
    12481 atttccagta tggcactatt tatactaatc acggcaattt ttaattttgt taatttaaca
    12541 acagcctatt ttatcagacg taccaaagag gttggtttgc gtaaaacatt tggcgctgat
    12601 aaatatcaga tcgtcaggca gtttttaatg gagtccattc tgattacttt gattgcgtcc
    12661 gtattttctc tggtggtgat ggatgtctta ttgtattttt taaatagcac ttcaaatttt
    12721 catctgaata gttctttgtg gagtagccca ctaaccattg tagggttctt attttttagt
    12781 ctatttgttg gtattattgc cggattatat ccatcatttg ttttatcacg acttcagcct
    12841 gctcacatca tcaaacaaaa tggagcaact tcaaaagcct ttagcatcaa cgtgcttaga
    12901 aaaaccctac ttgtattaca attcgttatt tccgcaattc ttattattgg gactggtatc
    12961 atttataatc agctttcatt cttacaaaat aaaaaccttg gtttggaaaa ggaacgggtt
    13021 gtcattctgc cgattagtcg tatatctgag cagaattaca acactttaaa atttttattg
    13081 ggacagtcat ctaaaattct tggcgtttcc ggttcaataa gtattcctgg tgaacgagtt
    13141 atttatgagg gcataatacg acaaggaacg gtagaagaac taggcgtgcg cctcattgtt
    13201 gctgattatg attttactga aacttatggt atgacactag ttgaaggacg taatttttcg
    13261 aatcaaatgt caagtgattc gtcaggtgct tttataatta atgaaaaagc caaaaatctc
    13321 ctagaataca aagaagtaat aggaaaaact ttagaagttt ccggtttaaa gcaaaaaggt
    13381 gcggtagtcg gagtcgtaaa agactttaat tttacgagcc tacgatctga gatacagcct
    13441 ctagtgatct ttcttaatcg cagcactatg agatacaatt atatatcgat taaatttatc
    13501 ccgaacagta cgacctcggc tattcagcaa attgagaccg tttggatgaa ggtatttcca
    13561 aacattccat tcgaatatta tttcctggat gacgcttttg aaagtttata tcaatcagaa
    13621 atcaacctca gagcgattgc gggcatcttt gctatattgg ctatggtgat cggggcgctc
    13681 ggattattgg ggttggtctc cctttcagtc ggacaacgaa cgaaagagat aggtatccgt
    13741 aaggttttag gagcaacagt ctttgagatc gtagcactaa tatcgaagga ttttctagtc
    13801 tatgttattt tagctaacct gatcgcttgg cctataatat attttggaat gaaacgatgg
    13861 ttagataatt ttgcatatcg gatcgaaatc gatttcatga tcttcgcaga ggctatgttg
    13921 ttgtctctcg gcataacaat tgttactgtg gctttccaag ccttaaaagc tgcaataaca
    13981 ccgccggtag agacattaaa gtgcgaatga aatttaaatt tataatttcc taccccaaaa
    14041 ctttatcgcc atgcactaaa ccgttttcaa ttattcctct aacgttgata catcgccttc
    14101 attcaagccc aaagcgcggg ctttgagtac gcggcgcatg atcttgccgc tgcgcgtttt
    14161 aggcagcaca tccagaaacg taacgctctc cggccgcgcg atcggaccga tttctttggc
    14221 gacatgttct ttgagtgtct cttcgagttc tttgctcggt gtctgtccgg cgcgtaggat
    14281 cacatagctg tatatggcct ggcctttgac ttcatgcgga agcccgatgg ctgcggcttc
    14341 cgagacagcc ggatggctta ccaacgcgct ttcaatttcg gccgttccga ggcgatatcc
    14401 actgactttg atcacgtcgt caatgcgtcc gatcacccaa agatagccgt cttcgtcgag
    14461 acgggccgaa tcgcccgctt tgtaccaacc ttgtttttca aattctttcc aatacgtttc
    14521 cacaaagcgt gcgggatctc cgaggatcgt acgtgccata cccggccacg gtgatttgat
    14581 gatcagttta ccttcttcgc ctgccgcgac ttctttgccg tgatcatcca cgacggcgat
    14641 ttcatttcca aaaaagggtt ttgttgcgga gccgggtttg agcggaatta ccgggagcgg
    14701 tgttaccata aagccgccgg tctctgtctg ccaccacgtg tccatgatcg ggcagcgctc
    14761 ttgtccgata ttacgataat accatttcca cgcttcggga ttgatcggct cgccgaccga
    14821 tcccaacagc cggagcgtac tcaggtcgtg acgtttgggc cattgttcgc caaaccgcat
    14881 cagaccgcgg atcgccgtcg gtgaagtgta aagaatgctg atgccgtatt tttcgatgat
    14941 ctgccaccag cggttggggt acgggtgatt gggtgcgcct tcgtacatca tgaccgtcgc
    15001 accgttgatc agcggtgcgt agacgatgta gctgtgaccc gtgatccatc cgggatcggc
    15061 ggcgcaccac cagcggtctt cgtctttgat atcaaaaacg taccggtggg tcgtggatgt
    15121 atatacggag tagccgccgt gcgtatgcac caaacctttg ggtttcccgg tcgtaccgga
    15181 tgtgtaaagg atgaaaagca tgtcttcggc atccatgact tcggtagggc acttgggatt
    15241 ggcgatgggc aaactcatca ggtcgtggta ccagaaatcg cgatcgtttt ccatgcgcac
    15301 ttcatgaccg gtgcgcttga cacagatgca tacctcgatc gtgggggaac gtttgatggc
    15361 ttcgtcggcg atggttttga ggtcgttgat ttttccgcgc cgccatccgc cgtcggccgt
    15421 aacgagtacg cggctcttgg catcttcgat ccgagcggcc aatgcttcca cgctaaagcc
    15481 gccatacacc acgctgtgcg cagcgccgat tttggcgcat gccaacatag cgaaaacaag
    15541 ttccggtatt tgcggcatat agatcgtcac gatttcgcct ttgcgtacgc ccatgctttt
    15601 gaggacgttg gcaaacttgg atacttcgcg attgagtgca tgataggaat aggttcgcac
    15661 atcgccgggt tcgccttccc agatgatggc taatttattt cggcgccagt ctctcaaatg
    15721 ccgatcaatc gcgttgagaa tgatattggt ctgtccgccg gtaaaccatt tgtaaaacgg
    15781 ttttttactg tcatcgagta cgcggtccca cgatttatac cactcgagtt ttccggcctc
    15841 ttcggcccaa aaaccttcgc ggtccgtgac ggaacgtcgg tacagcgttt cataatcttt
    15901 gatatgcgca ttggcgatga catccttgga aggatggtac atttcctgat tggatatagg
    15961 tttattgttg tcggtactca taatgcttct cctgtggact tgctcgtgat ggggttcaat
    16021 aaatgaatgc tataaattta acggttctta ttacgaatcc aagttttttg gataaaaaat
    16081 ttggcgaaac tatatgagaa agttatgagc cgaaatcctt tgcggaatgg agataactaa
    16141 cggatttcag tgtatacaaa aaagatgcag ggaaaaattt tacagttaat ctcacaaaaa
    16201 attgaactat tttttgtata aagaaattgc caatttatat ataacgaacc gattttttat
    16261 atcatccttt ttttacaaac atgatttgca atccagagat tttaaatttt tgacataact
    16321 gtctccttat aatatacatt caacgaaacc tgcatgacga aaaagatcat tactctctat
    16381 ttgtctattt tgacactttc atcttgtgct catcaaaaaa gagtactttt acatgtgcaa
    16441 ggattgtcca atgaaaacta ccaaacgcgc tcttttcaaa taaatggcag ttctggcgta
    16501 ccgattgaaa cgggtatttc ggttgagcag ggagattttg tcttcattga agttttaggt
    16561 gcgatacatc ttggctcgga cggtggcctt accaatggtg acggcatttc aggatactca
    16621 aagcaaaata agtataacta caaacacggt tcggtggtta cattggttgc cggaaaagat
    16681 catggcatga caaccatttc actgtttcgc catccgattt attatcaatt ggtagatttt
    16741 gcaaagctga cggacggtaa ctatttaccg tgtctgtatt ttttttcaga cacttcagga
    16801 aatatttctt ttgaaattaa tgatgtagat tattcaaata atagcggtga atttaccgta
    16861 aatgtatatc atcttaaaaa gagcggcgat ttgggcagaa attcatataa taagtgcccg
    16921 atgaaagaac cggaaaacgg taaagactgt gagggtaata cttgggtaaa agaaggaata
    16981 aaaggttggt tttatcatgg actttttaat aactcatatc gaggcacagg taagaatagt
    17041 ggttgtcagt gcgtttatac taaaggtgat ttagttgttt cgcatccgga tcaaggtacg
    17101 tttgattatg gattttggga atatcaaggc gatatagatg atgttcaagc cagacatttg
    17161 cacttgatat gggatgtatt gcctcatgat ttatggattg gtaaaaaaac atacaatgac
    17221 cctcatataa tctgcaaatg aggtcatata tattttagaa tcagaattga tctaatacgg
    17281 cggtttgtgt cggttgttat ctggtcttct atggattcat tttacgatgg gtgctttgaa
    17341 ccttcgcgcg atcaccaaac ccaaaatcgt cagcatatat cttataacct aatcgaaatc
    17401 agactatgaa cggtaaaatc aaatacgaat tttcagccaa aacctggcag catccttcac
    17461 ccaacggttg gcactttgtt tctctgccga tcaaaatggc aaaagaaatt agagaaaact
    17521 ttaagcggca ggaagagggt tggggacgac tgaaagcgac ggcgaaaatc ggaaatacgg
    17581 aatgggaaac tgcgatatgg tttgacacca aacacaaaac atacatactt ccgctcaagt
    17641 ccgaaattcg gaaaaaagag aaacttgagc tgaataaaaa cattaaagct atgatttggg
    17701 tttaaatata gatttgaacc cctgttctaa aaaaccacag taccgtggtg tgatcgttgt
    17761 gatcttcggt ataatgcgtg cgtgactaag actacgggac gtcgcgcaat acaaaccaaa
    17821 tgctctcgca aagccgctaa gaattctaat ttgtatttag ctttgcgtct ctgcgagatt
    17881 ggttttttga ttaaaagttc gaaagtttat tttataattt aaaatcaaat tccaaaaaaa
    17941 gttgagcgtt ttgtagtgtt ttgagatttt ttaattgtaa aagcatatct ataaacaaac
    18001 gcattgaaca ctaaaccatc aaatattaag aaacatgaaa caaaaagaat tgtcaaaatc
    18061 ggccgaaaaa gagctatctc agctaacaga tcaagagttg ttggttgaag cgaaaaaaat
    18121 gaaatccttt tccattaata acgctttgtt tgtcgggttt atggcgggaa ttattattta
    18181 tagcgttgca aaaaacgcct ggggattttt tactctgatc cctgtatact tcatctataa
    18241 gttggttaac gatccgaaaa acatcagatc aaaagtttta gaaagattgt tgaaagaaag
    18301 aaatttgaaa taaatgaaga tagttgggga ttaattttat cggaacaaat acatctagta
    18361 aatatgggta ttgcaaaaca tcatatctca ttatcccaca attacccgca acgcagcgtg
    18421 atcaccgtga tctccggcca gatgcctacg cgtccgggca ggccgataaa tccgaatccg
    18481 cgattgacat acaatgcacg cccgttttca gtatataatc cggcccatcg cgggtagcga
    18541 tactgcaccg gactccatcg gaatccggga atttccacgc cgaattgcat accgtgcgta
    18601 tgaccggata acgtcagatc gatgcgcgat gcgtgttgca aaacttctcc ttcaaaatgc
    18661 gtaggatcgt gcgagagtaa tatctttggc gccgccggtt ctataccctc cagcgctttt
    18721 tttagatcgc ctttttgcgg ccacggcggc agaccccagt tttctacgcc gactaaagcg
    18781 aggtgtgcat cgtttttgga aattaccgtg tgggcattat tgagcatcgt aaatcccatc
    18841 tcggcgtgca tggattggag ccacgcaagg ttttcggctt tggcttccgg cgaaggccag
    18901 gatacataat ctccgtaatc gtgattgccc agcgtggcgt atttcccgta atccgcattc
    18961 agtgttttaa aaatattcag ccacggcgta agttcttccg cacggttatt gaccatgtca
    19021 ccggtaaata ccagcaagtc cgagcgttgc gcattggcca gatcaacgcc gcgtttgacg
    19081 gcatcggcat cgtcaaaact gccggcgtga atatcggaaa gctgggtgat cgtaaaaccg
    19141 tcaaaaaccg aaggaagatc tttgataaaa acatcggtgc ggtgcacggt aaaatcatat
    19201 tttcctcggg taataccgta gagaatcgaa ccaaaaggta tcgcggccag accaaatccg
    19261 atctgcgcga taaatttgcg ccgcgaaggc aaaaaagttt cttccttagt ttttgtgcgc
    19321 cggacgacag accaagcttt acgtaaccaa cgcgtcgcat cttcagccag cagcagcgcc
    19381 accaatacaa gctttggtac gagcaaggcg acaaataatc ccatgatcgt cgcacgcagc
    19441 ggcataggcc ctaagcgaag cataaaaaac aacatgccgg tcgtcacaag gacgtacata
    19501 agcacattgg ccacccaaaa gctgcggatg atgatgtgtg attttttcca atccctaaca
    19561 agtgtacgca aaccggacgc aacatactta tcaatcaacc acaaaacgag gatgacggct
    19621 aaaatgatca atattccgtt aaacgaaatc atcaaatatt ctccataagc atacgaatta
    19681 aaacataata cggtgaagta gggtttcggt tgcggtgcat ttatagttaa tacgccgcgc
    19741 tgcgtaaatc aatatagagg ttctctctaa aaaacaaaag cgttccgtat gcaatttgcg
    19801 ttgaaattaa cgcaggattt atcaatcttt agcggtaatt atcgaatttt tcggagaatg
    19861 acagcgtgaa acgacttgga atattgctgg atgccgtagt agccattcgt cagatgggcg
    19921 gcggcaaaac gcccgatcca ttgcatgtaa tatccatgct ggaaatggcc ggcgtggata
    19981 gtatcgtata taccatgatg cagacgggcg cggcagaaga gcgcgatctg cgcatgctca
    20041 aagaaaccat ccattcgcat tttaacctgc gtttggtacc gaccatggaa aacgcgcagg
    20101 ccgccattca ggccaaagcg gagatggtga ccatgattga ccgtgacagt acttccattc
    20161 gcagtgtcaa tgccgttgaa aaagaagccc tactggctcc gatcgtaagt tttctgagaa
    20221 cgaacggtat cgttgtaaac atgctcgtag ataccgatgc caatcaattg cgcacggcgg
    20281 cccgcatggg ttgcgactac gtcgagctca ataccgataa atacacgggc gctgcgagtc
    20341 tgacgctgat ggagcaggaa ctcgaaaaca tgaaagcgat ggcgatggcc gccaaaaaac
    20401 tgacgctatc ggttacgctt tcaggtcaca tcaatcacaa taatatccgc gagcttctgg
    20461 acatcgaaga aatcgaagaa atcaatatcg gtcactctgt ttttgcgcgg gcgctgtttg
    20521 tgggcctcga tcaggccgtg cgcgattttg caacactcat caaaaaataa tccaatagga
    20581 gtatatatgc attctatgac gatgtacgat ctcatcgaac gtttagacga agaggaagaa
    20641 gaaaaagaaa ccgaagagcc gcaaaaagaa gatccactcg gaaaaaagat ggcggaattt
    20701 tttctgcaat cgcgtacgat tttgcttttt gaacagatca gccccaaagt cacgcgccgt
    20761 gtgatcacgc agcttttgtt tctcaacgaa caggattcca aaaaagaaat caaaattttc
    20821 atcaattcac ccggcggtgt agcggatgac ggtttggcta tttatgattt gatcaaattg
    20881 attgacgcac cggtcaaaac catcgtctgc ggattggctg ccagtgcagc cgcgatcatc
    20941 cttgttgcgg ccaaaaaaga aaaccgtttg gccttgccgc attcccgcat catgattcac
    21001 caaccgctcg gcggtgtgcg cggttccacc aaagacattg aaatcagtac gcagcaaatc
    21061 gttcgcctca aacaagagct caacgaaatt ttagcgcgtg aaaccgggca acccgtcgaa
    21121 aaagttgcgc aggataccaa tcgcgattac tggtttaccg ccgacgaagc caaagcgtat
    21181 ggcatgattt caaaaaccgt aaccggtctc agcaaaatct gagaggagag aatacgacat
    21241 ggctaaattt gaacatcata tttttatttg taccaacacg cgggaagacg gacatccgcg
    21301 cggaagttgt aataccggcg gaacgaacaa tctgcaaaaa ctttttaatg aggcgctgaa
    21361 aaaatacgac ctcaaaggaa aagtgcgcgc caataaagcc ggctgtctgg atcaatgcga
    21421 acacggccct aacgtcgtga tttatcccga taatgtgtgg tatggttttg taaaggacgg
    21481 cgatgtggaa aatatcgttc gtgctatggc cgaaggcaac cacgcgtccg accggatttt
    21541 gccggacagc tgtcttaata cgtcggcatg tccgcaccga aaaaacggat cagcgtaaaa
    21601 gaatatcctg taagacgtag aatgagccgg tgaccaccaa aaggtcatcg gcttttagtt
    21661 tttgtagtgt ggtatcgtag gccgacaccg tatcggggat cacttccacc ggttttcctg
    21721 tttgatgaat caacgcggcg agtgcatcgg ccggtagagc gcgggggttg ggcggtgtca
    21781 ctgcgaccac acgatcaaac agcggtacga tttcatgcaa agcgtttcgg ctgtctttgt
    21841 cggaaagcat tccgatcatc agtattttct gaccggtaat ttctttatgc agtaaagcta
    21901 tagaccgggc cagtgaacgc atacctgccg gattatgcgc cgcgtcaacg ataacccacg
    21961 gccgtttgcg ttttataaaa caacggcctt tccagatcac cgcgcgtatt cctttacgga
    22021 tatcgtcatc cgaaacggaa aaacgggttt gtaatcgtgc ggcggccacg gccaatgcgg
    22081 cattgtccaa ctgatgttca ccgatcaaag gtatattgat ttttgtgatc gtgccctgac
    22141 tatcagaaaa tcgccactgc gcgtccatgc atccgccatc aataccgact tcagaatttt
    22201 gaattgttat atgtgtaccc agcacatata gaggcgatcg gttttcgtgc gctttttttt
    22261 gcatcacgga gaatgcttcg gacggtatgt tgcctacgat gacgggtttg tccggtttga
    22321 taattccggc tttttcggag gcgatttttt ccaacgtatc accgaggtaa gccatgtgat
    22381 caaaatcaat cgtagtgatg acggatactt cgggttcgac gacgttggtt gcatcaaggc
    22441 gtccgccaag gcctgtttcg atgacggcga tatccacatg ctgctctgca aaatactgaa
    22501 aggccataac ggtcgtcgct tcaaaaaacg tacattgcaa acgatcaatt tccggttgta
    22561 aaagacgcgt caatcgaacg atatcgtttt cagagatttc aatattattg atgcggaggc
    22621 gttctgaaaa atgaacgata tgcggcgacg tgtataaccc gcaacgtaaa ccggcggctt
    22681 caaatatgga agccagcatc gcacagacgg aaccttttcc gttggtaccg gccacatgaa
    22741 tgacagggaa ttttttatga ggattgccga caaactgcaa aagagcgcgg gtattttcga
    22801 ggccgaactt tataccgaaa aactggaggt cgtacagata cgtaagggtg gttcggatgt
    22861 cctgcatgcg cgtgtgacgt cactatatat gaaaaaacaa ggtcgggata ccgcgtttta
    22921 acggtttaca tcatcgtgcg aggggatcgt atgcgattcg acgacttcat tattttgatt
    22981 gagaagcact actttaggat gaaaagcccg tgcttcggat tcttccgccg cgccataagc
    23041 tatgatggaa atcacgtcac cgggcatggc taaacgagcg gccggcccgt tgagaccgat
    23101 aacgcggctt ccgcgttttc cggcgatcac gtacgtttcc aaacgggcgc cgttattggc
    23161 attaacgatc tgcacttttt cattatgcca aatatctacg agatccagca aatcctgatc
    23221 aatagtgata ctaccgtgat agttgagatc aagttgagtg acacgggcac ggtgaatttt
    23281 agcgcgaaat atcgtacgga ccatcggttt ttacttattt tattttcaaa agttatcaaa
    23341 aattgcgcgg caatataatg gcttaaacgc aatcgggcaa tgaatatttc atcggtataa
    23401 gataaaaacc atctcatgag ctttcgtatc tatacgagac ttatgcgatg gtttttcaaa
    23461 gtcaactatt gcggtttatc ggatatagta ggcgataccg gcaccggcat tgattttacc
    23521 cggatattcg ccctgaagcc aactgtgttt tccgtttgta gaaaattcgt ccgaccaacc
    23581 aagatcagtg atcagactaa gcccgcttat aagacgaacg ccgattctta aattcgcttc
    23641 atagacccaa ccccggctgt ctcctgctga caaatcaccg taaggtttgg cggttcgtgt
    23701 gatgtgatac acgccaatct caggagccaa atccacgcgc tgctttatct gatattcatt
    23761 tttggtaaat aatccgaaag accaatcatg cgaggacaaa tcgccattca acccttcatc
    23821 aatcacatcc gaattatagt ttcgtgacgt ataacccaaa tgcgcataaa cgggtatctt
    23881 tcgtgcgccg aatggccaaa tattgggtct taaaaaccat ccggtcatgt gggccgcatc
    23941 gaacggatca gaggatgggc ctttataaga ttcattagga tctttggatc gcgttcccca
    24001 atgaaaaact ccataaccgt atttcgatga ttccacgcgc aaggcgtatg cgtattcact
    24061 gattttgtcg ctgctcgtcc ataggcgata aaacgatgca ccgtacacgg gattaccgga
    24121 ctgtgcaaat gccgtattat accaagttgc caaaagcatc ataccgaaaa aaagacgttg
    24181 tttcatgtgt tccttttatt tgttgttata gtgttatgtg caatccacaa cgcaataaag
    24241 cgaactatgt ttcaaacact acacatttgt gtaggcctca ttaaaatccg gaaatatcct
    24301 cggcggttaa tattttaccc accgggtaca gatttacatt tttatcccac catggagagc
    24361 gttcataaaa ccaattgagc cgattatatc cggtaaagtt tttatcctct ttgacgcgtt
    24421 tttcaaactc tgcgcgcagc gcgctgtctt tggccagcat atcgcgggct agcagttcca
    24481 gtttgtaggt ttcgccgtat tctttttgct cgaagatcgc attaaaaaat ccccattgga
    24541 ctaaggcatc aggagcgcgt ggttcgagta aatgcatgat cacacgtgct gtgcgttgat
    24601 tgagtttcac aatccggtca ccggctctga agcggcgcgt ttcttcggtt tcggaggttt
    24661 tataatttac cgacaaacgg ccttcaaacg gattttcaga ccatttggcg ttggagaatc
    24721 ggtatgagat cactttgata tcgcggtctt tgtctaattt ttccgttacg atcccgtgcg
    24781 cttccagtct ttccaatacg gatgtccact gctggggaac gatgaatgca tacggtacca
    24841 atgcggaatc ggtagttgat acctgatcat aaaaaggtaa gcggtaatca atgggcacgt
    24901 tttcccaata cacaaacgat tcgcccgtca cggcactttt ttccatgcgg aaattgtagc
    24961 cgagaaaatc aatcgtatcc gctttggacc agcggaaagt aatcggtaaa taagatccgg
    25021 tcatgcgctc cgccgaagca tctccatcca acatggtctg tctgagttcg ggtgcatgcc
    25081 gatgcatcgt ctcaagagtg tgcaggatga cattgtatgt gccgtccacg cgggttttgt
    25141 aatctttgaa catatgcgtt tcgatcaaaa aaccgatacg gttttgcaga gcggcataac
    25201 cggtcgaaaa tcgcggaggg gaattgccac cgagcaaacc ttgagcgata tcccgctctt
    25261 cgacgaggtt gacatacggt gacatcggaa aaccggattg cgccatggcg tttttcaatt
    25321 caggcatata gacgtctttt gtaaacgacc gcactgacgg agcgaggttt tgcgactgat
    25381 caatagcgta cgtcaccgca tgccgataat cagcgccgtc ggtgacatgg caatccaata
    25441 agatatcggg tttccacgcc tgaaacagtt tgagccagtc gcgcatttcg ggcgcatcgg
    25501 ctttcatgta atcgcggttg aggttcagat tttgcgcggt ggtacgccaa cccatttctt
    25561 tggggccgtt ctgattgatt cggttgtacg gaccaaaacg ctcgtgcccg tcaacattga
    25621 aaaaaggcat aaataaaatc acggtatgat ccagcagttc cggatttttc ttaaaaatca
    25681 cgagatcacg aatcagcatc attccggcat ctttaccgtc tatctcaccg gcatgaatgc
    25741 cggcttgaat caaaagtaca ttttttccgc tttggcgggc cgcctgagga ttgaaacggc
    25801 gatctttatc cacgatgaca agcgggttca tacggccttg cgggctgacg ccgaagtacg
    25861 tcagattgac ccaaggcgag gcctgctcaa ggcgctgaag gtaagcaacg gtttcgtcgt
    25921 aacgcggggt ttcgaggtga ttggattttt cgtaatacgt cagccattct tccacgcgcg
    25981 gcggcttggg tgcacatgcg ccaagaagta ctaccgataa cataagaagt cgcaggacaa
    26041 gttgattcat gatttcctct atcgtttttt ttcttcgatt aataacatca tcgtatccaa
    26101 aagccggata tcgttgggcc aggcatcttg ggaaatattt agttttgtac gaaaatgcgc
    26161 cgaaattgtt ttggcgagcg ccattcgacg catcggttcg aatgcgtttt ttctgtaaaa
    26221 aaaggagcgt agcacaaaat actcatcatc gttgatccgg tttaaattgg ccagcgtgat
    26281 accccgaacc atttcgggat tgagatgcag caacccgaaa ttttcaaatc cggtcgttgt
    26341 acgttctttg atcaccaatg ttccggcggc aaaatcgcca agccgtttac cttgcggatt
    26401 accgatcaaa accgcgctgc cgagcccgta actgggaaaa taaaaccatg tgggcaaagc
    26461 atcggcgatg cgcatcagat tgcgaatagc cgcatccatg aaagtgatcg ggtacccttc
    26521 gtctttgacg acgcggaggc ggaggatttt tttgccgatc gtctgtccgc gccagacggt
    26581 ttcaaaaaaa ataaaatatc cttcataaag caagaaaata ccgatggcca aaagtgcatt
    26641 acgccatcca tcggaaattt cttcgatatg taacaagccg ccgataaacg cgaaggctat
    26701 ggctaaaaca taaaccaaaa taataaaaaa aatcgcctgc atggccgtat caatcaaata
    26761 cgctaaaaaa cgagagccaa ggccggccaa ttcgaactcc aaaaaaatat tttcgggtgt
    26821 ttcgacgatc aaatgcgatt cgatgtgcgg ttccgtattc atgcttcgat attaatacaa
    26881 cgtatttttc tggcgaacat acaagtcccg gtcatactcc gaggcggtga tgcgctgcgc
    26941 actcaagtct tccaaaatag tacccgtgct gggaagctga tcgcgggtga tatgcaaaga
    27001 cggatcccac aggccggaac gctggatggc acgtgcacat tggaaaaata ctttttcgac
    27061 gtgaacgatt aaaacggtcg ccggaagttt gttttccatt tcaaatcgtg atcgccatgc
    27121 cggatcgacg gtaatctgtg cgcggccgtt gacacgaagc gattcgccga tacccggtac
    27181 cagaaaaaaa agcgctacgg aaggattgtg aatgatatta cgcaggctgt ccatgcgatt
    27241 attgccgcgg cgatccggta tcaaaagtgt tttttcattt tcgacggtca caaaacccgg
    27301 cttatccccg cgcggtgacg catcgagccc gtgcgggccg gatgtcgcta acacaaaaaa
    27361 aggcgacact tcgataaatc ggcgataatg cggatgcatg taatcggttt ctttagcgag
    27421 cgatgcttcg ctgggtgtgc cgtataattt ttttagtgtg tcctcgtcgg taacaaggaa
    27481 ttcgcggtcg ggagaaaaag tagaatgatg catgcgttga taaaatttcg tgttttttag
    27541 aattgaaggc tcatctcagt agccgagtcg tttcgcttca tcccaatatt ggtccatttc
    27601 ttcgagcgta gcagttttca tatccttttg attttcgaca tacttccgtt cgacatattg
    27661 aaaacggcgg gtaaattttt cgatcgtacc gcggagcgcg tcttcgggat tgatcggcat
    27721 aaaacgtgcg agattgacga cggaaaaaag tacgtccccg agctcatctt ccattttttg
    27781 aagttgtccg gatcgtgcgg cttcttctaa ttcttttact tcttcgtgta cttttttcca
    27841 tacttcgtcc acgtgacccc agtcaaaacc gacacgcgag gctttttcct gtacgcgata
    27901 cgcccgtacc agtgcgggta aaactttggg tactccatcc aataccgagg tgcgaccggc
    27961 ttctttgagt ttgatctgtt cccaattctc cagcacttta tcggcggttt tagcttcgat
    28021 gtcaccgaat acatgcggat gccggcggat cagtttatca ttgatggttc gcaatacatc
    28081 caccagatca aaatggttgt tttcggaggc aatgcgcgaa tgaaaaagaa tttgcagcat
    28141 gaggtcgccc atttctttgc gcagctcggt ataattattt tcatctaagg cttcgagcgt
    28201 ctcgtaggct tcttccaaaa gatactgtcg cagtgaggcg tgcgtttgtt ctttatccca
    28261 agggcatttg gcacgcaact cacgcatgat attgtataag cgaccaaact gcgtacgtac
    28321 ggcttctgtc gaagcgtcat catccaatac tattttttcg gtttcgtgtg aagattccac
    28381 cgcttctcct atcgggtcaa atttattttt ttctgtactt cttcgttgta agcgcgttca
    28441 taggcgccga ctaattcgac atattcgggc gtctgacgca gggcgttaaa gtttttatcc
    28501 ttgagcaacg cgttaaagtc cacaaaacga tttctgaaag cgcgactcag ccatcgcagg
    28561 gcggaagtgc cgtcatgctg ggatgcagcg atactggcca atcgaaaaca agcctggatc
    28621 gtcgtcgtat cggcgccgta ggcgagagta tatttttgag cggccacatc gtatttcttt
    28681 tgctgaaaat actgttcgcc ccaatcttca tacaccgtgg ggtctttgac accggcgctt
    28741 aacattcgcc gctccgtcgc cgccagtgac gcatcggcct cttcccatcg ctcgagcgtg
    28801 ctgtaccagc gtgcgagagt ctggcggtac ggatagttgg acgaatccag ttcgaccgct
    28861 ttttgtagtt ccgggagcgc tttgtcgtat ttgcgaagcg ccatgtgggc gcggcctaat
    28921 tcgtaatgcg cctgtgtata gttgggaaat actttgacgg cccgttcaaa tgcgcgaacg
    28981 gcttcttcgt tattttgtgt accgcgaaac gcgatacctt gaacgtaata cgattcggca
    29041 ttggggtaca cgcgaatgct tttgatgatt tcgatttgcg cttcttcgta ccgcttgagc
    29101 ttgttaagcg ttttagcgag cagcaaaaat actttggcgt aggtatagct gtcttcggca
    29161 tgctcggcat aacgacgcag gaaaggtaat gcaagatcat acgagccgac ctgaaaatag
    29221 gcttcaccga ggtagtagtc caaccggtat gcgcgcgaat tcaacgattg cgcggatttg
    29281 aattcatgga tggcttcggt ggggttttgc aaataataca aatatacata accggaagtg
    29341 atgcgcagtt cgaactgctt tttggggtcg gtggcgtcgt caagaatgcg ttcatctacg
    29401 gaatccatca atgtttgcag aggtttgatg tctttgcggt tggcggacat ccactgtagg
    29461 atttgtatcg ggttttcacg ttggttttcc gattcgggtt ttatcatcac ggcgctcaca
    29521 taataccgtg cggcgtcaac atagcgtttt ttctgataca tgagattggc caaagataat
    29581 cgcgcggtga tgtcttgcga ttccagagtc aatacttgtc catacatgga tacggctttg
    29641 tcccagcgac ggtcaaattc gtataaacgc gccaggtttt tgcgcgccat gatatcatac
    29701 ggattggtgt tcgttgcttt ttcgaagtat tcgcgcgcac cggctgtgtc acgttcggcg
    29761 accgcgacat taccgatcag attccatgcc gtagcggttt cccggtaaac ggttttaccg
    29821 aatttcttct gccatacttc gacggctttg ttggcgtagt tacgcgcaat cgtataatct
    29881 ttttccagat aggcgcagta acccaattgt aaccaacttg cagcgtctgt cggatcgcgg
    29941 tgcgtagctt caaaataatt agcctttgcc gacttgagat tgtttttatg atcgtagtgc
    30001 acatccgcta tttttcgata gatataaaag cgcgccgaat ccagctcgag agttttttgg
    30061 taataactga tggcggcatc gtattcgttg cgcgcggcgg ccaattcgcc ggcgcgttca
    30121 tacaagagcg aagcgcgcgg ttccttttgt aatgcttttt gtatccagct gtctgcggtt
    30181 cgcgtatccg accatgcggc aaacgcttgc tgaagcatcg tgtacgcttc atcggagcga
    30241 atattttgta aatctttttt taccgtttcg agggcttttt tatcggcgat attaaaggat
    30301 gaaaaaatat cggtcatcat tttttcatgc gtcgcacgaa gttgtcccgg tttggtttta
    30361 taggaaaaat ctttttgaag gctgtgcagt gtgtcgcgca ccgtcatctg aaagcgaagt
    30421 tcattttttt cataaccgca cacaacccgg atgacgccgt cataatattc gggtgctccg
    30481 tttgcggacg gatggatttt gaaataagag gacttatcca aggcgggtac cagagaacgg
    30541 tggagcgccc gtgccgtcca tgcattggag tcggaagcca gcggttcttg gatttgtaca
    30601 tccagccaca acgttaccgg cagcgattgc gcggatacca aaccggataa gccaaaaaca
    30661 aaaataaaaa acaatgattt catgcaatac tccggcttaa tttggaaagt gatacgtcat
    30721 aacggtaact cgtacccatg tggccgtcgt caaattcttc ataccaatgc gggatactat
    30781 agttacgaag tttatccgca aatatacgcg caccgatatg aagatggtat tcatcacggg
    30841 cgccacaatc taaaaaaata tgattgatct tagaaagatt ctcgcgccag gctttttgat
    30901 ccacgcaaaa aaccggatcg tgggcgatcc agcgttgcca tacggactct ttgagcgcgc
    30961 cggtttgcag atcaaaaggc aattcgatac cgtaaggttc ggatgcttgc ggcgaataag
    31021 ccgctgccat ggcgatgata ttgatagccg tgatctgttc gccggatttt tttggggctt
    31081 caaaaaattt ttttaaaaat ccgtccagac caccggtttt atacagggca ttgtaggttt
    31141 tgggaatatc gggtttgtat ccgtattcga aatacatatc gccggcgctg caaaaaaaag
    31201 cggaaaataa ttccgggtag tttacggcaa gacgcagcgc accaaatccg ccgctggatt
    31261 tgcccatgat accccggtga tttcgctcgg tctgtgtgcg aaatgtacga tctatgtaag
    31321 ggatgatttc gcgaatgata taatcttcat aattgccggt agcgggcgaa ttcatatact
    31381 gcgcaccgcc ccaacgggta aaacaatccg gcatgacgac gatgcattct tccatcgcgt
    31441 gttcggcgat gagcttatca caacgttcgt ctatcgcata accccaggct tgcggtgaca
    31501 gcatcatcgt accgtagccc ataaatccgc tcaagaggta aataaccggg aaccgccggc
    31561 tgggggctgt cgtataggaa ggcggcaaat acacggccaa tttgcgctgg tgcggatcat
    31621 tgagcgggtt gttttgcaaa acttcgcttg tcaaaacttc aatgcgtacg gtgccgggca
    31681 ttctttttct ctttgttttt ggaggaatct gaggatagtt tttcagataa tcaagtgcag
    31741 tgccataccg ttacgacata gtaaagtatt tttgcgcttt gatgtttact gcaaagaggt
    31801 aggcaattga gattagagta gtttaagggt tggattggat tctgtttgag tggcaaattt
    31861 ttgaagccac ataataaaac attagatcaa aaaatcatga tgaaaataaa agaagcaaag
    31921 ttagtgcgga cggatttcgg cgaagttttt ttgataggtt tatccgttca aaatatcatc
    31981 cgcccagctc atggcggcca tcatgcctgg aaaaccgatg gtaggaaggc attggagaat
    32041 ggtgtggcga atttcttccg gcgtagcgcc tgcatcaagc gctttgcgta catgggaatg
    32101 gagcgctcct tcctgacgcg aaccagcaga aataccgatc ttgatcaacg ttcgggtttt
    32161 ttcatctagc gggccggcat cttgtacggc atgactcaat gccgaatagg ctttaccgac
    32221 ttcgggatac tccttcagaa aacgctgaaa acgttttggg atagcacgtt ttggtgtatt
    32281 cttttttgta acgcgtggca tacgtttaat ttttggtttg tgaaggtttc gcaggctgat
    32341 atcgcagaac accggtttcg atgggaatag cgatcttgac gagcaatgta aaaataaaca
    32401 tgcccatcgc ccaaataccg agcgcgacga aaatttcaac ccacgtcgga gcatattcga
    32461 cgattttgcc ccacggtccg ggaataaatc ccggaatgat caaacccatt cctttttcga
    32521 tccagatgct tagaaaaagc aaagcgcatg cgggcaaaag ccatttgtga ttactgcgta
    32581 acggattcat tgtaagaata gcggtggcaa taagatttaa cccgatggct gtccatatcc
    32641 acggctttag cgcaccgaaa ccgtgtaatc cgaaaaacag atagacggcg ctttcgctgt
    32701 gatgcgtcgg tttgtaaaat tccttaaaaa gttcggaacc gagcatgatc aaattgattt
    32761 gtgcggcgac ggttacgatc agtgctattt ttcccagtgt tacattttca accttatatg
    32821 ccgtatagcg gcggatgacg gataaaacaa tgatgattag cgccggaccg gctgcaaaag
    32881 ccgacgccaa aaatcgcgga cctaaaagcg atgaattcca aaacggtcgc gccggcaaac
    32941 cggcgtacag aaatgccgta actaagtgaa tacagaacgc ccagaatacg ctgataatga
    33001 caccgggtac ataaactttg ggattgggat gttttccctg ataatggcac atcagaatgt
    33061 aaaacgggat tgtaagattg agaaataaat aaccgttgag tacaagtacg tcccacgtaa
    33121 gcattgagct tggccagttg aagcgaccga tgccggggat caagtgccat gcacgacccg
    33181 ggccgccgag gtcaactgtc acaaaagcaa gacacatgat cagcgccgaa acggcgagac
    33241 cttcaccgat caggacggct ttagaaaaat caacatcctt gagaatgtac gtcggtaaca
    33301 ccagcatcac cgcggccgcc gccacaccga caagaaaggt gaaattggaa atataaaaac
    33361 cccaactcac gcgatcgttc atacccgtga cggccaagcc ttcgcttaac tgcacggagt
    33421 aagcataggc gccggccaac ataaaaaatg tcagtatgcc catccagata tgataaccgg
    33481 gcctcccgcg tgtgatgctg tcaaacgcgt cgcgaataaa cgaacgcgcg atgacgggac
    33541 ggatgtcatc ggttattttt cccgcttgat gcggcagatc aggcgtaaca agatgttcca
    33601 tagtctaatc catgaagtac cagaatttgg gttcggttcc caaatcttct tttagtcgaa
    33661 atactttttt attggcaaga acccagcgga tttcgctgga agggtcgagc aaattaccaa
    33721 agatacgtgc gccggtgggg catgcttcga cgcaagccgg atttttcccg ttacgggtac
    33781 gctgaatgca aaacgtgcat ttttccatca cgcctttttt gcgaaggcgg ttaccgagat
    33841 agtgttgatt aagattgacc tcattttccg gtacgaccgg atcctgccag ttaaaacggc
    33901 gcgcatcata agggcacgct gcggcgcaat agcggcaacc gatgcaccag ttatagtcca
    33961 ctacgacgat gccgtcttgt tctttccatg ttgcttgaac agggcaaacg tctacacagg
    34021 gcgggttttc gcagtgcatg cattgcgtgc cgatataaaa atgaccttcc atcgggactt
    34081 cgtgataaaa attgtccggt gcatcgctaa agtccatccg gcctttttta tgttcgtgca
    34141 cggtgatgta ctgcataccg gaacggcgat cctgattatt ttctttgatg catccgcgga
    34201 tacactccag ataaccgcga cacttggaaa tattgaatgc ataaccgtaa agtacgccgg
    34261 gaatcgggcc ctgatcggag atgtgtgcgt gttttccggt atgcatttca tacatgcgtt
    34321 ccagacggct aacggcctct cttttttccg cgtcactcat catccgaaaa ttgccctgaa
    34381 aataatcgtc ccacgccagt tgtgcttttt cacgatcttc tgccggagca agcggattgc
    34441 aggcggtttg cagtaatcct aagccggcga cgaatgccgc ggccatattt tgtaaggcag
    34501 aacggcgatc catggattgt tctaacacgt gatcagcggc tgttgctttg ggatcaggtc
    34561 gtaccgacgt atggtcatcg cggtactgac tccagcgcat gacattttcg agcgacggcg
    34621 tcgcacgcag atagtcccat tcggtgtctc gtttgagcca gtcggagaga acattttctg
    34681 caagtaaatc acgcagacct tctcggcgcg gatccggcgt atgttggcat tggcaatgtg
    34741 acatgctgta tccgttataa aattatttga tcaatcgttg catcgtcaga ctgggctgcg
    34801 ccggccaacg tatatcccaa cgcggctgat gcggattgtg gcattcgaca cataacgcta
    34861 cggtgcgcgg aggcgcccaa ccgccgacac gtttgccgtg tgcaccgccc tgccaatcgt
    34921 tgaattgacg cgaatgacat tgttggcaca actgataact atcgttaaaa gcgatgactg
    34981 caccggtgat gctgcgcagg ttattcaaat ttcgttcatc gtgacaaaca gtgcattgca
    35041 gaacgttggt cgtcgcatgc tgtagatttt tatcccagtg cgcttttcgg cccgagggat
    35101 cgctagattt catctgtccc aaaggtttgg tatggcattc cgtacaggga tattttttga
    35161 ttagcggtgt gcggggtacg acgtagagag gtcgctctac aagctgcacg gcatgcaaag
    35221 acgcagtatc tgcggtcgga gcgacatgcg tataagcgga atccgaccat gcgggagcgg
    35281 tatctaattt ttccaaaagg ccttgtgcgt gatcgtgtcc gcaagaaaca aaaaatacag
    35341 ccagacagac accggccata gtgcgtagaa ttttaaaatt gcgcgtattc gcagaaaaca
    35401 acatgtgttg tcgcataaat ttttttgtta agcgattacg gagttggcgc cggacgggtc
    35461 caggcggtat tatcttcgat agctccatga cattggcggc agttgacacg atccggatgc
    35521 ggtgtgcgta tttcgcgaac ggatgccggt ccgccgtggc acgccaaaca attttcccgc
    35581 atctgaaggt catgcggtat ctgcggcggt gcaccggtca gagcggaacg attgatagcc
    35641 ggcggtttca ccggcgtaaa tcccgatgcg cgaaatacgt tgcgcgtcgt aaccggtacg
    35701 tgacactgac gacagctcaa aaactgcggg tgcggcgata ccggagcata tgcgttaaac
    35761 ttgggcacaa aaccgccgtt ttcatggcat tgcaagcagg tgttaccacc gacactgcgt
    35821 tcatctgtca cttcgtgcgg tattaccggc ggtgcaccgt caaacgcccg gcgctcataa
    35881 aacgcagcaa gcgtgcgtga tccctgttga tggagagtct caagataata aagcgcttga
    35941 tcgctttgct taaaaacacc ggcttcatcc ggaagcaccg cttcgcccgt tgtttttgcc
    36001 gaaggtgtgt aggcttccgc tccggactgg atgacgcttt gccccacgat cacgatgacg
    36061 cacagcatca gaagtgcgct cagtgagata aagatcacgc ggttttcggg gagcaaaagc
    36121 ttcttgaggt tcatgctctt tccaccttta cggcgcattt tttgaaatcc ggctgccgcg
    36181 aaataggaca aaaggcatcc agagttaatt cgttgataag gtgggactcg tcaaaaaacg
    36241 gaacgaacac cataccgtgc ggagggacac cgcgctcatt gatcgaagca ctcataacga
    36301 tgctgccacg acgcgatgtc aggcgcactt tgtcgccatg acgaatacca agttctttgg
    36361 catcgtcggg attgagttcc acgtaggaag aaggaacggc ctgatgcaat acagggatgc
    36421 ggcgagtcat cgaaccggag tgccaatgtt ccaaaacgcg tcctgtattg agccagtacg
    36481 ggtaatcatc gtccggcatt tccggcggcg gttcgtacgg tcgaatccat atccaagctt
    36541 tgccttcagg tttgccgtaa aaatcccacg catcgccttt ggctgcagga tcgtaggcgg
    36601 catggtaacg ccacttcgtt tctttgccct cgggacatgg ccaaagaacg ccgggattgg
    36661 ctttgagcat ttcgtacgta gccatctcgt gatctttacc gtgatgaaac tgcgtatatt
    36721 ctttccatat ttcttcaaca tagtttttcg aatcccacgg aaacaacgcg ccgtgaccga
    36781 gccgacgagc tacttcgatc atcgcccatg catccgcaat gcattcgccc ggaggttgca
    36841 gcatttgttc gaaatgctgc gtgcgtcgtt cggagttacc aaacatgcct tcgcgttcga
    36901 tccacatggc gctgggcaaa atcacatcgg cgatgtcggt ggtcggcgtc ggatagacgt
    36961 cggagacgac gataaaacga tcttcttttt tggcaccgtc gcgataacgt ttcagtttgg
    37021 gcatggtcac catcggattg gtaacttgta tccacataaa acggatatcg ccgcgatcca
    37081 gtgcgcgaaa catttctacg gtgtgatatg taggtttggc agggattttt tcaacgggca
    37141 ctttccagat ttcagccgcg tgttgacggt ctttttcatt ggttacttcg ccgtgcggca
    37201 aacgatgtgt aaaacaaccg acttcacggg ccgtaccgca ggcgctgggc tggccggtga
    37261 gcgagaaggg actgtttccc ggcgtagaaa tttttcctgt cagcaaatga atgttataaa
    37321 cgaggttttg aatccatgtg ccgcgtgtat gttgattcat ccccatacac caaaaactgg
    37381 taactttttt attggcatcg ccatagaatg acgccagata acgaatatcg gatgccgaaa
    37441 ccccggaaac tttttctacg atttcaggtt tatagtcttc aagaaatttt ttgtattcct
    37501 ccaacgaagc accctgcggt tcatcttgga atgcaaactt atcttctaaa ccatatccta
    37561 tttgcgtttt accttttttg aacgtcgtgt gtttttgaat aaaattctga ttgatgcggc
    37621 ctgtgcgtat cagttcgtag gcgatggcat tggcgacggc aagatcggtt tgcggtttga
    37681 ataagattga tttatcggcg gccatactgg tacgtgtagt acgtgtggcg aaatcaataa
    37741 ttttggcgcc gcggcgcagg cggttatcaa gcattcggga aaaaagcacc ggatgcattt
    37801 cggccatatt gttaccccag aggatataca catcggcatg atcaatgtct tcatagcaac
    37861 ccatcggctc atcctgaccg aatgtcgtca taaaaccggt tacggcgctg gccatacaca
    37921 gacgtgcgtt gcactcaagg ttattggtgc cgataccgcc tttcataaat ttggaggcga
    37981 cataaccatc cggtacggtc cactgaccgg aaccgtaaat ggccaccgaa tctttaccgt
    38041 ttttgtcaat gcattccttc atcttttgtg cgatgagatc gagcgcttct ttcatcgtgg
    38101 tttcgacaag ctgtccgttt ttccggatga gcggtttggt aagacggtct ttggcgtaca
    38161 acgccatcac ggagtgataa cctttgatgc agcataagcc gcgattgacg gcgctggccg
    38221 ggtcgccttt gacagcgacg gctttaccgt tttgaatacc gatcagaagc ccgcaacccg
    38281 ttccgcaaaa acggcaaggc gattttttcc agtccaattt ttcatccggc aaaaaacgtt
    38341 cgataccttg ttcgccggaa aatacaattc ccggaaaaat cgatgcggct gcggacatag
    38401 cagcgacagt agccatgtgc ttcagcagtt cccggcggtt catgccaagc ctccttgcat
    38461 attcgtattc gtagtggtgc ggtcttcgta tccggaaacc aaactcagaa accggatgct
    38521 ttcgatttta agtaattttt cttctatttc tttttcttgc gcatcatcgg atgtatcggt
    38581 tacaatgaca aaaacatcgt aattatccga tggaactatt tcgcaattcg gcaattcttt
    38641 cagcgctttg gcaagcgctt cgcgttgatc ttgcatcgga tatgctaaat agcttttaac
    38701 aggcatgata catctatcta ttaaggtatg taacgctaaa cgattctaag tttgatgtga
    38761 atgttaatgg aagagtaata acaatcgaaa gtatgtaaat aagtaagagg taaaaatgat
    38821 ataaatcata caaaagactc ctgaatctta atacgaacaa caaaaaaatc catgtaaacg
    38881 ttttaaaaac aattgattga ggcggtttgt gtttgatcaa tcaaatatga gtgaattttc
    38941 acttattcac tttggttctg aaaaaaatta gtattaaaaa gcgtatgaca gcctgtctga
    39001 ttcgtattta ttgtttcatt tcctcgtgaa ttcgcgcaga ttttcgcgtc ttctctataa
    39061 taattaaagc aatcggctaa gttagaatac agcttaaaaa ccggttattt aaaaatcata
    39121 aaatgttttg aaacatttta actgcaatta cgaataatat attgcaatcc gatattatat
    39181 atttatattg tctgcaattg cgaataatat aaagacatta acattatgaa aaaaacgaaa
    39241 ctcggcgaat ttgaagaatt ggtattgctt accgtgattg cattgcgcaa ggacgcctac
    39301 ggtgtggaaa taaaacgaga actggaagaa cgcctgaacg aaacattgag cgtcggttcg
    39361 atccaatccg ctctgaagcg gatggaggag aaaggttatc tcacttccgc atttggtgag
    39421 cctacgcaga agcgaggcgg taaacgtaaa cgtatttata cggcgactcc ggcagcacat
    39481 cgcgtgttat cggaactcaa atccattcgg gccgggctgt ggaattccat gccccggctt
    39541 gttcatacgt gaggtaaata tgcatacacc cgattccatt caaccgccgc gttggccgat
    39601 cgctgtactg cgatttcttc taaaaaaaga atacctcgaa gaaatagaag gcgatctgga
    39661 agaacatttt gccgacgata tcgaacaaat gtcggtgttc aaagcccgga tgtattttat
    39721 atggaatgcg ttaacgcttg tcaggccatc ccttctcaga cacatcggtt cgtttcatat
    39781 ccagcatcat ccggcgatgt ttcgtaatta tttcaaaatc tcattccgta gtctgatgcg
    39841 tcagccgttt cagtcgttta tcaatatttt cgggctttcg gcggcgatcg ggatttgtat
    39901 tttcacgtac gcattttcgc gctggaccta tagtatggat cagtttcata cgcataaaaa
    39961 tgaagtgtat ctcactacgt tttatgccaa tagggatgga tccgatcagc agtttggccg
    40021 ttcgccggct ccgct
//
LOCUS       DAOQGF010000016        39685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_430_length_39685_cov_7.350820, whole genome shotgun sequence.
ACCESSION   DAOQGF010000016 DAOQGF010000000
VERSION     DAOQGF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 39685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_39685_cov_7.350820"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK937_04070"
     CDS             <1..386
                     /locus_tag="PK937_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31778.1"
                     /translation="IAKKEGIPENGIHAALRSGMLSRESFAKYGLGSMLKRTFDNNLR
                     KAYLPYILPDADTREQLMKDITLLQLIKRQLRLFDEQPILIRIVRYMFYRFAQNSVER
                     TYRRIYERLRKRTQRRLDEILAMMG"
     gene            487..1716
                     /locus_tag="PK937_04075"
     CDS             487..1716
                     /locus_tag="PK937_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNH31779.1"
                     /translation="MINEKEPRFLLITLIATILFGFWFVTIKDVFSPFILATVIIAFL
                     LPFRDHKTIRVLIALIFTLFMLWLIHELREIFTPFLIAFALAYLFDPVVDGFEKRKFS
                     RTSSIVVIILAILGTLFAIGLLVIPQFAGEVSALAGTFPSYEDFKRNLNPDALSFLQS
                     WGMDTAKIMTMIESETTNKITGLIREFTQSAQSLSDGLSSLITQLINLILIPFITFYF
                     LRDFDQNIARIRSRTPERHRAKFEKLYTRIDGILSVYIRRIFLTAFTLGMLSMIILFV
                     LDIRFATIIGITLGLFYLIPYVGYLLTFAVGAIMGLLNPDPGSAVLKIMIVIACIKII
                     DLGIVSPRLVGEKLGLHPVLMIFSIFVFGKLLGLLGLFIAIPVTAILKVLIMEWYDQN
                     FLHREFIRDDSSSTSKE"
     gene            1719..2495
                     /locus_tag="PK937_04080"
     CDS             1719..2495
                     /locus_tag="PK937_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31780.1"
                     /translation="MGYSVKDRVILITGASAGIGEATARTLAEAGGKLILTARRKEKL
                     EKLANELKEKFGTSSYTASLDVRDNAAVENFVRTLPDAWSAIDILVNNAGLGRGLDKL
                     HEGKIADWEEMIDTNVKGLLYVSRAVIPGMVARGRGHIFNLGSIAGHETYPGGNVYCA
                     TKHAARAINNGMRIDLVDTPVRVTTIDPGLVETEFSVVRFRGDAERAKKPYEGIAPLR
                     GEDIADLILFAATRPTHVNINEMIVMPKAQATSTLVHRKV"
     gene            complement(2498..5848)
                     /locus_tag="PK937_04085"
     CDS             complement(2498..5848)
                     /locus_tag="PK937_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH31781.1"
                     /translation="MKLTEFLFRYRRSIGPVATLVAIVILELAAGQIQNYRPGSIALQ
                     ALIFFSVLLGGLRSGLVSAVLQFLYRLYYMLDFGHPYNEAPEQFWTIVFWIIVNTFAV
                     LAMAWLMQRWHAADARRIQRISQDYDVLLESSSDAVFIMGSDRRYLSVNQKACVMTGY
                     SREELLTMGPLDLIDPDTAHTLDTTLALMQKEGKYLGEQRFKRKNGALFYVEISAKVL
                     NDGRALGIVRDISDRKRREQQLYESEQRFRALIENSSDVVTLVDQRGTILYTSASIKR
                     LLGYETWEISGRSAFDFIQSDADTVQRIFESVRRRENRRVHLSVPLRHKNGSIRWVEF
                     SAINMFHVPFVQAIVTNYHDITERRFAQEALRQSEYNLSGVIENTTDSIYSVDRNLRV
                     VVLNSAFRVAYESAYGVILQPGMAILEYLPKATADEWRSYYQRALEGERFVIEKLFEF
                     AGISHYYDISFYPLRDAQHIITGVSVFSRDVTERMQSRKMISDSEIRYRQMFENNTAI
                     KWLVEPESGRIVDANPAAAAFYGYTVDQLKSLRVWDINVLPEKEVRERIAQAMEAQQT
                     YVDFQHRLANGEIKEVEVRTGPVVIGERKLLFSIIHDVTERRKVEQALIASENKYRSI
                     FENITEGIYQATEEGNFITINPALVRMLGYKSHDEVMRLNLNRDIYVRAEDRLQFNEN
                     TRAQGIGRYAEVFWRKKDGAIIQVRLHDRAVYDPGGRFLYYEVAVEDITESRRLEQNL
                     IQAQKMESVGRIAGGVAHDMNNMLAVILPTAEMLKNFSTSPEMVKTYAENIAASARRA
                     GEIVKQLLVYARQTPLRITRIHINDLIRETQAMVTHAFPKQVRFDIRLDDRIPAMDVD
                     QTQIQQVIMNLLVNARDAMGEPGGLINVRTHSVEFDGESAELLNLPGAGVYIECIIRD
                     SGVGMDAGTLQNIFEAFYTTKPEGQGTGLGLAVAKSIILNHGGTIMVDSKKGEGAEFR
                     FYLPASRSNESAPVPESEKPLETGKETVLVVDDEKAILNICSRILEELGYRVITAQSG
                     MEAVRLYQQEKPDVVMLDIQMPEMDGKQTHAALCGINPMAKIFFATGYATPDVVRDIR
                     QNLKSTIIEKPFSIDTLSKVIRTLLDQN"
     gene            5965..6648
                     /locus_tag="PK937_04090"
     CDS             5965..6648
                     /locus_tag="PK937_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNH31782.1"
                     /translation="MTRAQKLYTILCGIFISALIVAEITGSKLIQHSVSENWVFILTM
                     GTIPFPITFLVTDLINEYYGKPGIRFVTLLGMVLIGFVYVILFIDMRIPAAAISPVSD
                     AQFNAVFGQSSRIILGSVTAYLIGQFIDIQIFHTIRKHTGEKWLWLRATGSTFVSQLI
                     DSFVVLFIAFSNTLSFEQIMTIGATNYIYKFVIAIILTPVIYLTHAMIDRYLGVSESH
                     TMIAQSVEK"
     gene            6669..7136
                     /locus_tag="PK937_04095"
     CDS             6669..7136
                     /locus_tag="PK937_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNH31783.1"
                     /translation="MPEILCRVIDCHVFRQTQEGPRYLVMQRANEVIYAGSWRMVGGK
                     IENGEKAWETALRELSEETGLTAMRLWAVPYINSFYEASKDRVNIIPVFAACVQNDNV
                     TLSREHDAYRWASYDEALTLLPWPAQIEGLRIVHEYITTGKTVAGFVEIPLRA"
     gene            complement(7125..8561)
                     /locus_tag="PK937_04100"
     CDS             complement(7125..8561)
                     /locus_tag="PK937_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31784.1"
                     /translation="MANNRIRNLVSIRSLPGLYEMRRGDRTSAMVMLLSWLLPIVMII
                     YSWQAFLTGWHALVTVPILIVTNFEALTRLWSPEIMEHAVASLAASGWIIGVFYWYHK
                     KRLITQQRTSASPWRLAGQAFRRNGFAMCFLCILMVLVLMAIFAPWYAPYDPNAQQDI
                     VVTKYIKPMHSVTALRLKRNDIVPPRPSAQNFSEYWTATLQSINQQFRQTEEPLLFVD
                     EWHLQGNEVFYTQGIQKKKLDISNLIGTEPIIFLTQRMYWLGSDRFGRDILSRLIYGT
                     RISLAIGMLAMVMAVALGTLVGTIAGYFGHRIDGILMRGVDIMMAFPNLFLILMIVAL
                     FGNSVLLMIAVLGLTGWMGVSRIVRSQVLVLRNSEFITAARALGYSHARIMRVHIIPN
                     TLAPVVVAATLRLGAIILVEAGLSFLGVGVQPPTASWGNMVSDGRDALLHAWWISTFP
                     GLAIVLTVMSFNIVGDGVRDALDPKLRA"
     gene            complement(8551..9039)
                     /locus_tag="PK937_04105"
     CDS             complement(8551..9039)
                     /locus_tag="PK937_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNH31785.1"
                     /translation="MTQKPLYIFDGYNLIKRIDALRREEIKSLEQGRQALLVHIATFR
                     NMTNHDAVVVFDGKKMSLQAKAGVRILFSNLPNRADEAIKRLVDDQAKARTITVVSSD
                     NEVMWYAQGCGCRVERSEDFFQRLTTLKNKPVSDELSAKNDPSLSEREMDYWRSMFEH
                     GE"
     gene            complement(9036..10181)
                     /locus_tag="PK937_04110"
     CDS             complement(9036..10181)
                     /locus_tag="PK937_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH31786.1"
                     /translation="MKNKLNQWRALFPSTQHGVYVNHAACSPVSTRVKSAIDELLTVS
                     STTHVDNFPVWMETRESLRGILAQMLHTLPARIGFVKNTSAGLNILADGLSWERGDHI
                     LIAEGEFPSNVYPFLKLQDRGVRVEFVPTPTGRFTTDDFVSRFTAATRLVSVSFVQYH
                     NGFRADLEALGRACHARGILFCVDGIQGVGAVPLDVTKTHVDFLSNGGHKWLMAPGGL
                     GFVYVGEQLIDRLTVQQVGWLSVKHPWNLREYALDFPEDARRFELATENMLGIYGMKA
                     SVSLLMEVGIENIFYHITALIDLLAQGLNEQGWIIRSDLTSTHRSGIISFQSRNEQSI
                     PTKNVFEKLMQSGITVSYRDGAIRVSPHFYNTENEIRQILEKLNAWV"
     gene            complement(10178..11095)
                     /locus_tag="PK937_04115"
     CDS             complement(10178..11095)
                     /locus_tag="PK937_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNH31787.1"
                     /translation="MKIVLAGATGFIGNALIKALLEEKHHLIILSRRPDVLRNQWGDT
                     VKAVFWDAVSSGDWFSDINEADAIINLTGASIAGKRWDASYKKIIADSRILSTRILMK
                     ACHVAQQKPKTWINASAVGYYGDVPEGDVDETRHASDDFLGRTCAAWENEVLQAETLG
                     IRVVCLRIGIVLEEGGGALEKMVLPFKLFVGGPLGSGRQWLPWIHRDDLVRAVLFILN
                     EKTIRGPVNGTAPMPVTMKQFSQTLGSVLRRPSWAPVPGFVLKIVVGEFSDSLLAGQK
                     ALPVKLQNAGFRFTFDELHAALRDIFIKK"
     gene            11573..13027
                     /locus_tag="PK937_04120"
     CDS             11573..13027
                     /locus_tag="PK937_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH31788.1"
                     /translation="MKSYRVFFLLLILFVGIGIGLILSFNIGKPRLSAQNSSTADSFP
                     IVHISQALASELAATSFSGIARLSTPAVVTITSEKKQSSSDEETQFRFHRDMPEQGVG
                     SGVIVSADGYIVTNNHVIDQAERIVVRLADRRSFEATLLGTDPLTDIALIRIDATGLP
                     TITFGNSDMIQVGDWVIAVGSPLSLNSTVTAGIVSATNRQIEIIGDQYGVENFIQTDA
                     VINPGNSGGALLNMRGELIGINTAIASRTGMYQGYGFAVPSNIVRNAVQDLSRYGRVV
                     RGYIGVGIKDVDAIYAKAIGLDKARGVLVESLTEKGAARHSGVEEGDVILQIDDMPLF
                     QRNELQAYVALHKPGESVRLQIWRDGRTVEKKVMLRNPDGNTDIPAEKIQRQNDAHIG
                     SESWGYGLGFEVENLSDQGMRGVMISKSSWRAKAQNIYEGMILTRIQKTDINDVSVFE
                     KAARELKNGEAVIIQLADRRNPANHILTAVEVRR"
     gene            complement(13058..14689)
                     /locus_tag="PK937_04125"
     CDS             complement(13058..14689)
                     /locus_tag="PK937_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNH31789.1"
                     /translation="MENSTSEASKPDSVSQSGSDEPPQSNDSQTKTGSLQDEINRIES
                     RIEFSIRKYSAAEIAIIGFAMAILIGSVLLFLIENRGENVISYVDALFISTSAICVTG
                     LTSVDFSKFTFAGQVITMVLIQVGGFGVVTAFRLLWIGRTGNLSPGTAQTLGSVMDVE
                     GEHRTIFQVLASTAKITLAIEGLGCLLLYLATKDRLADGPITPLWFSVFHTISAFNNA
                     GFGLYSDNLMPFRSDVSVNMVIAGLIVLGGIGYPVLLGIESFFLLMLHKMLNFSEALM
                     EMIAMRRPDKLYLVEPFYNFVDRLYLRSKKLREELAGVASPLQMKIVIWGTVFLLAIG
                     TVLMAAYEWDDPHTLGQYDAGEKWLISFFHSVSTRTAGFNTVDIGLLQIRTLMVFISL
                     MFIGAAPQGTAGGIKIPTFATMLGYIRSSFRGMNRVSLFRYVVSKGSVGNAVKLYVLS
                     STFVLSVILLMTVLETRFTLTQMAFEVVSAFGTVGLSAGITMLMTPASKILIVVTMFV
                     GRVGLLTIGSAIIKPTDSSPNTGTWTDDGMKLQIG"
     gene            complement(14828..15220)
                     /locus_tag="PK937_04130"
     CDS             complement(14828..15220)
                     /locus_tag="PK937_04130"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNH31790.1"
                     /translation="MSFIWKDEYLIGDKEIDDQHKNIFHFLTDLEEGVTNGRSDELVS
                     QAIDFLSAYIRDHFKYEEKCMHERKCPVAEYNEHSHKKFLEAYEKIADKYQHDGDSIQ
                     LLREIQALVKVWLVTHIIRIDSHLKKCS"
     gene            complement(15497..16612)
                     /locus_tag="PK937_04135"
     CDS             complement(15497..16612)
                     /locus_tag="PK937_04135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH31791.1"
                     /translation="MNWKRICSEFLEIGVNNDYLQKRMDDFRNMSVVMFILGAPFAAG
                     LWEWDYFRDPVGAEQTFLLRLAFFPIFIGVAMVMYRIKDYRLVGIFSVIAMLVTEVFL
                     IQIFERLESGVIYGIGGFMFYFLVSLIGFLGLPLRLTIPYAIAITAFPHLFAFFGYFG
                     GFQHVNFATLIWPAAVLTILGHIAATHNYRKRYEFEISLELATKTDPMTGAVNRRGFM
                     PLLEQEFFRHRRIGHSLALLMMDIDHFKSINDTYGHPTGDQVIRMLANTCEKFSRQTD
                     TVARLGGEEFAVLMPHTEKNMAVMLAERIRIGVEECSQVSDSGIPFRYTVSIGISECQ
                     PDDKTFDQLIARADTALYRAKTQGRNRVIVSGDFDES"
     gene            complement(16645..17127)
                     /locus_tag="PK937_04140"
     CDS             complement(16645..17127)
                     /locus_tag="PK937_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31792.1"
                     /translation="MKVLNAKFFICYKSLIIFLIVLLTFGALSCSCKGSDVIKIPDDE
                     FRYFLIATVGNDFFIVRTSDSVIQSVADSLLLLPLTDRNMMIVGPIDRGNEDYNYDWN
                     WHFKTNAWHFAETAMELCDGSPEYVEEHLDEWLSTVGTYCPWGSRIKKELFAGDVIQK
                     "
     gene            complement(17229..17936)
                     /locus_tag="PK937_04145"
     CDS             complement(17229..17936)
                     /locus_tag="PK937_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31793.1"
                     /translation="MSESTINKAIRAYGGEACWKKAKRIEAEISVRGLAFTLKQRPFF
                     EHALVQMEIARPVSSIIPIGRDPSIRGRLDSEDVWLEDVSGKVLETRNSIRRYFPFGK
                     HAFRWDDLEMSYFANYAMWNYLTLPSLLMNEEILWSETEQGIVKAVFPTSIPTHSKNQ
                     EFRFDKDTGLLCEHLYTVDIIGRYARATHTILAHNEDTGLIYPSRRKVTPQTPWRTAL
                     PGPILIDIEIHRFRLIC"
     gene            complement(17936..18322)
                     /locus_tag="PK937_04150"
     CDS             complement(17936..18322)
                     /locus_tag="PK937_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31794.1"
                     /translation="MDWLKKEGVPVLLASLWIGVSEFLRNEIFFKTYWTNHYLSLGLK
                     FPSDPINGAIWGVWSVFLAILIYVLTSRFSLFQAWLIAWFGGFVLMWIVIGNMGVLPS
                     GILYFAIPWSLLEVLGGALIIRKLAK"
     gene            complement(18518..18928)
                     /locus_tag="PK937_04155"
     CDS             complement(18518..18928)
                     /locus_tag="PK937_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNH31795.1"
                     /translation="MDSNGKQLQMFYSKALTVNNETNPAEILNSILADQFVSLGSVES
                     KNKMQLIMQLVFFWKLIPDLKWEIQEIINEGNKYVVRSMASGTPNGDFMGMPTDGTKS
                     FKIMTIDIHTMVGGQIAAVHHLEDWPTAIKQLKA"
     gene            complement(19048..19917)
                     /locus_tag="PK937_04160"
     CDS             complement(19048..19917)
                     /locus_tag="PK937_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH31796.1"
                     /translation="MKEKIETRRFYRNDSRLEVEVVAIEKLKRIGRVVLSKPHRTDFH
                     HVFLVENACTHYVDFKKIKIQPYSLLFIAKGKVHAFDPSIKLQGRIIIFTEHVFSVSE
                     SDVSFLKNTPLFDSLYEIFTIPLSKVLFAKFSTICEDIREESDSNAMRSEPVLVKNLL
                     QNFLIIAERTLRDQGFREKRKDADWDEVVRFRELLENRFTEHKTVFEYADEMGIGLKK
                     LERLTMRILGKSPKAIVHERLLLEAKRLLAYTNRSVKEIAHHLGFDDPSYFVRYFKRF
                     ANITPLEFRETFK"
     gene            complement(20039..20953)
                     /locus_tag="PK937_04165"
     CDS             complement(20039..20953)
                     /locus_tag="PK937_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH31797.1"
                     /translation="MSPVFRYVVQTSIGYSGMKNEYLKQAVNGDIQAFQKLFLEFQDT
                     LRSYLYRLVTDRHDVEDISQDTFVKAFDKISTFKGESSFKTWVFQIATNLAYDLLRDK
                     KRWKHDAQDQAKAYASSSTMMRQRFLDAHHYSPHGAYHVREHIDFCFTCIAKTLPIDE
                     QITLILKDIYDFSRKEIGMILGLSEGVIKHRLFDARKKMQTIFDSRCALVNKNGVCYQ
                     CTELADFFNPESDHRKTLQNEELIRRANTPDREKLYELRAKLVSQIDPLRSEGSDLQD
                     IIMQCTRIAIGEMGPASPDKKGRAETQI"
     gene            complement(20984..21787)
                     /locus_tag="PK937_04170"
     CDS             complement(20984..21787)
                     /locus_tag="PK937_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH31798.1"
                     /translation="MNLIEGKVAVIFAAGGAIASEVAQAMAMEGAHVFLSGRRLAAVQ
                     EIADRILSSGGKAMAHQVDAESENEIIAYIELIANLTGKIDIVFNGIGARPVDLGYGT
                     PSADLPFSLFMKTLQINAGSQFLTSRTAAKIMRRLGSKGTILTLSASLSRLKIPCMAG
                     ITAACSAVEGMSRVLAAEFGSSGIKVICINAAGMPTTRTIRETTALIERTTGVIPIEV
                     DKKTSSNALLGYGPCPKDIGNLSVFLATPAGSIFNSHVIDADFGAANVI"
     gene            complement(22107..23093)
                     /locus_tag="PK937_04175"
     CDS             complement(22107..23093)
                     /locus_tag="PK937_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31799.1"
                     /translation="MKKYWNASRWLIVMSMVNFLLVIGCSEKDSSNLTVDEEEIQNQI
                     IEDADEIIGVDILGESDATGYSKSYDGEAVVLSKSASPIDSIKGYGRVVRMSRRDIQI
                     TVAEDTMSAEAEITFLMKGNFVIRAENPDGEGLVKYTKPLEHEMKRIVRFVRNTDPEI
                     DRRWLVSEVSAGFGISKNGTMSLSSVNVTIHSAAGVTDFGFTDPLAYFIERGGLPQVS
                     QGDTVTVEVGITNSNADDAPMGWIHRGKNRNAKIAGMRVKHLFNDEGTYGDRVTGDGI
                     YTVRWIVGPTEFAGVHVGALDFVTHSTVYESDADAAPYNSLIVALPYRKVIN"
     gene            23511..23786
                     /locus_tag="PK937_04180"
     CDS             23511..23786
                     /locus_tag="PK937_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNH31800.1"
                     /translation="MKLKPLADRVIVEPMEAEEKTASGLIIPDSAKEKPQVGKVVAAG
                     PGKKKDEPLTVKTGDMVLYGKYSGTEITYENKKYLIMSESDILAVQG"
     gene            23843..25495
                     /gene="groL"
                     /locus_tag="PK937_04185"
     CDS             23843..25495
                     /gene="groL"
                     /locus_tag="PK937_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507618.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNH31801.1"
                     /translation="MAAKEIVFDVDARSKLKRGVDVLADAVKVTLGPQGRNVLIDKKF
                     GAPTVTKDGVTVAKEIELEDPIENMGAQMVREVASKTSDVAGDGTTTATVLAQSIVRE
                     GFKNVTAGANPMELKKGIDSAVKVVVAELAKMTKKVEGKEQIAQVAAISANNDNEIGK
                     LLADAMEKVGKDGVITVEEAKGYETILETVEGMQFDRGFLSPYFITDADSQEAVLEDP
                     FILIYDKKISVMKDLLPILEKTVQMGKGLVIIAEDVENEALATLVVNKLRGTLRVAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEDAGYKLESAKVEYLGRAKKVIIDKDNTTIVE
                     GVGKKETIKGRIAQIKTQIENTSSDYDKEKLQERLAKLAGGVAVIRIGAASEIEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRAQKALEPGRGGINDTLLKGDTGLGVSIVK
                     RALEEPIRQIVENCGIEASIVVQKVREGNDHFGFNALTEQYEDLLKAGVIDPTKVTRT
                     ALENAASVSGMLLTTEATVYEKKENKPAMPPMPPGGGMGDMY"
     gene            25761..29030
                     /locus_tag="PK937_04190"
     CDS             25761..29030
                     /locus_tag="PK937_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNH31802.1"
                     /translation="MKRNLWLCLWFALFTLSSAQAQQESRLMRFPATDGQQIVFTYAG
                     DLYTVPVNGGLARKLTSDANGYEMFARFSPNGQWLAFSGQYDGNTEVYVMPSGGGVPK
                     RLTITATLGRDDVADRMGPSTIVMAWKNTSEEILFRSRMRSFNDWNGELFSVNLQGDA
                     PSQMPVPRGGWMSFSPDDKKMAYNRIFREFRTWKRYRGGMADDVWIYDFASKTVENIT
                     NNNASDLYPMWVGNKIYFVSDRDARSRFNLYSYDITSKTTTQLTTFTDFDIKFPSLGK
                     DAIVFENGGYIYRFDLKTEKATKVPISIQEDFASGRNALLQVGKNVTNFEISPDGKRA
                     LLGAHGEIFTVPSKAGITRNLTQTSGTHERASKWSPDGKWIAFISDASGENEIYIMPQ
                     DGSAKPTQITKNADTYYYTLYWSPDSKKILWADKKLRLRYVDVATKNVTEVDQATFWE
                     FSEYTWSPDSKWIAYTRPEDAGMSRVMLYSLDQKKSTEITDGWYESGSPAFSHDGKYL
                     IFGSNRDFNPVYSWTEWNHSYQDMTRIYMVTLSKETDSPFKPKNDEVEIKKDDQPKAD
                     DKTKKDDAGKKADASVKIDLDGIKQRLIVLPVSASNYYNLAGVDDKIYYSRQGSKDEK
                     PLLFVFDLKEQKETELGSFNGYEISADNKKMLVGQSGSFAIVDLPKGKIEFKDKLDLG
                     NMEMRVNYREEWMQIFNECWRQMRDFLYDPALHGVDWEGVRKKYAPLVEHVNHRNDLT
                     YIIGEVIGELNIGHAYVGGGERKQPRRINLGLLGAEIERDPATKYYRIARILPGENWR
                     KEVRSPLTEVGVNVNNGDYIIAINGQSVAGVNNINELLINTADKTTLLKVNSKPSEQG
                     ARDVAVTPIADEADLYYYQWVQTNIEKVNKATNGEVGYIHVPDMGVNGLNEFVKHFYP
                     QLRKKALIIDVRGNGGGNVSPMLIERLRREIAMVDAPRNLSSRPDPFDMMVGPMVCLL
                     NEFSASDGDLFPYRFRKMKLGKLIGKRSWGGVVGIRGSLPLVDGGYLNRPEFGSYDTD
                     GKEWIIEGHGVDPDIVIDNDPAREFAGIDDQLTKGIEVIMEELKTKKRELPKKPEYPK
                     K"
     gene            29063..30715
                     /locus_tag="PK937_04195"
     CDS             29063..30715
                     /locus_tag="PK937_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HNH31803.1"
                     /translation="MKHFLITALLITMIACKPAEKPKPEKSEMQKKIDEFAKTHIAVN
                     MDHLSAKEKEMVKLLVKAGQIADGIFWKQTDADAVRLRDELKAKNDPASKEAYEYLMI
                     NYGPYDLETNRRFVGQGPTERPLTGTYYPADMTKEEFEAYIKANPKEEKELTGLYTVV
                     VRDGKKLKAVPFHEVYKAEVTAISQLLAQAAALCENVSLKTYLTERAKAVLTDDYYAS
                     DVAWMKLKDNNIDVVIGPIENYEDRLYNYKTAHECAVMVKDPEGTKELQMFQSHLDAL
                     EKNLPSKKEYIRSSAGKANVLEVVNIVYFGGDFQKGVKTIAASLPNDPKVHDEYGGKK
                     QMYKNLMEAKFQKILLPLGERLIAPDSRKYIDMHSFTTFVTMHEVSHTLGRSFVYGKD
                     DLPVRKALKELYSAIEEAKADVVGIYNMDYFAKQGIYDAETMRKHYTTYVVGLFRSIR
                     FGAEEAHGKANIAQLAFLQEQGALGRDAQGYWQIHPEKFQAGLSALAKVLLEIEAEGN
                     YDGAKTFIEKYGKLTDAIKADLAKFHDIPRDLNTTYDIMNQL"
     gene            30747..31616
                     /locus_tag="PK937_04200"
     CDS             30747..31616
                     /locus_tag="PK937_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNH31804.1"
                     /translation="MPITWINGKYVPEDEIALSPNDRGFLYGDGAFETMRSVQSRIIA
                     LHAHIHRLKTACERLRIPLTQGPEDFRTIIQELLNRNALQDAYIRISISRGPVEQFGF
                     GFSKTIKPTCLIAVRPAPTIPDNIYQKGISVGFQISSLFGSHTGETAIKSLSAVEYVL
                     AKQSALENKCYDMLLMDSIERVYEGTSSNVFIVRQGALCTPPVHSGILPGITRARVIT
                     LAETKLHLPVREILFSKQDVLTAEEVFLTNTNIDVLPVTVAGEQTIGNGVPGKITQSL
                     LQLYRQTLIEILE"
     gene            31609..33012
                     /gene="pabB"
                     /locus_tag="PK937_04205"
     CDS             31609..33012
                     /gene="pabB"
                     /locus_tag="PK937_04205"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNH31805.1"
                     /translation="MNNESAVHIEPWDWPDDIWSLYRTIAASPRFIFLYSSLLQPSYG
                     RYSYLACDPSIVLKCTSHHNEITTATGVTSIDGNPFDCMYDLMQQYAAPKNHANVDFC
                     TDGWFGYWGYDIRDHLEKIASRARKSLNTPDALWMKFDAVIILDHQEHQAWITGTRTG
                     IEKLRERLQQPVASYPEKPLQFKIHPTETRASYIQKINSLHESIAAGDVYEVNLSQRF
                     DVTVDGYDPNVYSRIFRSLVETSPAPFSAIIQCDETAVISSSPELFLQMHQNICTSKP
                     IKGTRPRTGNFDSDEKNYFDLQTDEKDRAENLMIVDLVRNDLGRVSQAGSVVTKDLFA
                     VETYATVFQMVSTIQSRLSEHATMMDAVKAAFPPGSMTGAPKISAMQHIESLEPYKRS
                     VYAGAIGWMQASGETMLSVVIRTLIFNGASGYFQTGGAIVWDSIPDEEYQECWDKAQG
                     ILRALEKLGVINLPVYK"
     gene            complement(33120..34139)
                     /locus_tag="PK937_04210"
     CDS             complement(33120..34139)
                     /locus_tag="PK937_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNH31806.1"
                     /translation="MKTLLRTMVIIAFGLLLSCSSRIRQAAYPTLFDNEYDSEFPYRS
                     SSDQLEEISASVKIMFSTAFYNSYYFDEKFAITPTAVRAGAAEKYAYRYLNTQASVSG
                     TATVIYYDTKRVALLTCAHVVEFSDTVYSYYPSGHVQSVSFLQRQSNFIRDLPEGGEL
                     EVLAIDSKNDIAIIGRSFDHIPQEPVHVFGYPIGQSKKLRWGSFVYVIGYPMGFKMVT
                     KGTVSSPNRDRNGSFLTDAPMNEGLSGGIVLAIKDGVPNFELVGVAKSISATQEYYLM
                     PDPGFTQEIPAPSLYKGELYARLKTEYRYGITHVVSTEILRSMYEKYKKSIDAKGYSM
                     DALFK"
     gene            complement(34366..35277)
                     /locus_tag="PK937_04215"
     CDS             complement(34366..35277)
                     /locus_tag="PK937_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNH31807.1"
                     /translation="MVMEMKLRNMSDRTVDVYTYRVRCFLMHFQKAPENIRDDEIGQY
                     LMFLRETKNYSWSGVNIVYSAIKFLYEQTLGVVINHFPMPRPTREKRLPVVLSISEVK
                     RLLTHEPNAKYRLIFMMIYATGMRLNELLHVRQRDIDRERMLILVRYAKGGRARYVPM
                     NATLLIHIENYLRTQPKQRWLFPGQIGDEPLADRYVQGLFRTLQKRSRLSKQLNVHAL
                     RHSLATHLYENGTDLMTIQRVLGHRSINPTLQYIQLQAREFIKIDHPLDHMQAFTAFP
                     KRLSRSAYADMRYRPWRRKINCFQESY"
     gene            35402..35872
                     /locus_tag="PK937_04220"
     CDS             35402..35872
                     /locus_tag="PK937_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome c
                     subunit family protein"
                     /protein_id="HNH31808.1"
                     /translation="MRGFTHLFFVHLNLVIFGMALITPCLGQSRSQADSVAAVRKLHL
                     SALNKTLEGRESLPADSVFKNLQTIGGFEAGLMPVIMEKWSIALGVGCDYCHDTNNWA
                     SDAIHEKKTARQMAGPLNEAIRNVLSKIDGLSERPVVNCATCHRGEVKPATRVK"
     gene            36099..37223
                     /locus_tag="PK937_04225"
     CDS             36099..37223
                     /locus_tag="PK937_04225"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNH31809.1"
                     /translation="MVIGIPKETAAGENRVAVTPDTAAKLIKMGFEVRVEKGAGQNAH
                     YTDEQYTKHGAAIAATATDLLGQSDIILKVQKPSDAEIKQIKKGALLISFVYGLNYPQ
                     VPKACAEAGVNLMSMDFIPRTTLAQKMDALSSQYNIAGYKAVLMCAAAFGKMFPLMMT
                     AAGTISPAKVVIMGAGVAGLQAIGTARRLGAVVEVSDVRAAVKEEVQSLGGKFIEVPG
                     AENMQDAGGYAKEASAEFLQKQKELLFKHITEADIVITTALVPGKKAPVLVTEEMIKH
                     MRHGSIVLDMAVEFGGNCEVSEVGKTVTKHGVTIIGESNIPATVPTHASELYSKNLLA
                     LLQYIAKDGNLQLKLEDEIVKSALLVHNGVVVNERIKQVL"
     gene            37245..37523
                     /locus_tag="PK937_04230"
     CDS             37245..37523
                     /locus_tag="PK937_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNH31810.1"
                     /translation="MGDIVMLITVFVLACFVGVELISKVPPTLHTPLMSGSNAISGIT
                     IVGSLISAGIGEMTISTIMGVIAVAFAMINVVGGYLVTDRMLKMFKRK"
     gene            37554..38996
                     /locus_tag="PK937_04235"
     CDS             37554..38996
                     /locus_tag="PK937_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNH31811.1"
                     /translation="MEHFRESIENIMYLIGAVLFIFGIKMLGSPKTARKGNFYSAVGM
                     LIAIVVTLFDKHILTYEWIIVGILAGSVIGIIFAVKVEMTSMPQMVGLLNGFGGGAST
                     LVALAEYYRGMATYQTALQSNPSLTLLEHMPYQTTVSIVLGILVGAVTFNGSLVAYAK
                     LQGLISGNSIRYPMQHPLNALMIVVTLVIGALGVMDLNNQWYFLTVTILGLILGITLV
                     IPIGGADMPVVISLLNSYSGIAAAITGFVISNNMLIITGALVGASGIILTDIMCKGMN
                     RSLMNVLLGGWGTAPTVEAAKDKKQVVVKSVDVEEAAMLFDAARLVIVVPGYGMAVAQ
                     AQHAVRELMNVLEKKGCAVKFAIHPVAGRMPGHMNVLLAESNVPYEHMYSLEEINDDF
                     RNADIALIIGANDVVNPAARHDQSSPIYGMPILNADYAKTVIINKRSMAAGYAGIDNE
                     LFGFPNALMYFGDAKDAVSKLTNELKSMNN"
     gene            39036..>39685
                     /locus_tag="PK937_04240"
     CDS             39036..>39685
                     /locus_tag="PK937_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31812.1"
                     /translation="MITNVHGDCPFYPLSFFMKRFTIVRYQHVVYYTGLYLVIFFWLG
                     SMPSVVAQRSFIMQNETGVITQDRPSGNSIGVITTTGDTVFIAAGSNLNITTNAGAAW
                     NIIGRSDGLYKGSPVAITHHKSVLYVTTAYDSSLAVEPTYGVSGGISISKDFGSTWSH
                     IAQPTDTGTIRGPVVRTDARTSQPVVLNLVRDVMRRQTGFLTDSIYVVAIKSAIDN"
BASE COUNT        11161 a         9553 c         8536 g        10435 t
ORIGIN      
        1 ggattgctaa aaaagagggc atccccgaaa acggcatcca tgcagcgttg cgcagcggca
       61 tgctttcacg cgaatccttt gccaaatacg gactcggcag tatgctcaag cgtacttttg
      121 ataataacct gcgcaaggct tacttgccct acattttgcc cgatgccgat acgcgtgagc
      181 agttgatgaa agatattacc cttctgcagc ttatcaaacg ccagctgcgg ctttttgatg
      241 aacaacctat attgattcgt atcgtacgat atatgtttta ccgatttgca caaaactccg
      301 tcgaacgcac ataccgccgc atatacgagc gcctgcgcaa acgcacgcaa cggcggctgg
      361 atgaaatatt ggcaatgatg ggttgattta tttttaatag ttacaaatta caagaatgta
      421 gctactgcct atctactcaa tttccccgtg cttttgtccc ctgccctgat tagattcatt
      481 gcaaccatga ttaatgaaaa agaaccccgt tttttactca tcacattgat tgctaccatt
      541 ctttttggct tttggtttgt caccataaaa gatgtgtttt caccgtttat attagcgacg
      601 gtcatcatcg cttttttact tccgtttcgc gatcataaaa cgattcgcgt gctcatcgcc
      661 ttgattttca cgctgtttat gttatggctg atacatgagt tacgtgaaat tttcacgcct
      721 tttctgatcg cttttgcatt agcgtatctc tttgacccgg tagtggacgg tttcgaaaaa
      781 cgcaaattct cccgcacgtc ttccattgtg gttatcatat tggccatact cggaacatta
      841 tttgccatcg gacttttagt cattccgcaa tttgcaggcg aagtctccgc gttggccggc
      901 actttccctt cgtacgaaga ttttaaacgt aatctgaatc cggacgcctt gagttttttg
      961 caatcctggg gtatggatac cgcaaaaatc atgaccatga tcgaatcgga aacgacgaat
     1021 aaaatcaccg gccttattcg tgaatttacc caaagcgcgc aatcgttgtc cgacgggctg
     1081 agttcactga tcacgcaact catcaatctg attctgattc ctttcattac attttatttt
     1141 ttacgggatt ttgaccagaa tatcgcgcgt atccgatcac gtacaccgga gcgacaccga
     1201 gcaaaatttg aaaagcttta tacccgtatt gacggaattc tcagcgttta tattcgccgg
     1261 atttttctga cggcatttac actcggtatg ctttccatga tcattctttt tgttttggat
     1321 atccgttttg ccacgatcat cgggatcaca ctgggtttat tttacctcat tccatacgtg
     1381 ggttaccttc tgacatttgc tgttggcgcc atcatggggt tgctcaatcc cgatcccggc
     1441 agcgcggttc tcaaaatcat gatcgtgatc gcctgtatca aaatcataga tctgggtatc
     1501 gtctcaccgc gccttgtcgg tgaaaaattg ggattgcacc cggtgctgat gattttttcg
     1561 atttttgtat tcggtaaatt gctcggactg ctcgggcttt ttattgccat tccggtgacg
     1621 gccattctga aagtactgat catggaatgg tacgatcaaa attttctgca tcgcgaattt
     1681 atccgtgatg attcatcgtc aacatccaag gagtaaatat gggctattcc gtgaaagatc
     1741 gtgttattct tatcaccggc gccagcgcgg gtatcggtga agcgaccgcc cgcacattgg
     1801 ccgaagccgg aggaaaattg attctcaccg cccgtcgtaa agaaaagctt gaaaaactgg
     1861 ccaacgagct gaaagaaaaa ttcggaacgt cgtcgtacac cgcttctttg gatgttcgtg
     1921 ataacgcggc cgtagaaaat tttgtacgca ctttacctga cgcatggtcg gccatagata
     1981 ttttagtcaa taacgccgga ctcgggcgcg gtttggacaa actgcatgaa ggaaaaatcg
     2041 ccgactggga agagatgatt gataccaatg tcaaaggttt gctctatgtc agtcgcgctg
     2101 ttatcccggg catggtcgct cgcggcagag gccatatttt caatttgggt tccatcgcgg
     2161 gtcatgaaac ctatcccggc ggcaatgtgt attgtgcgac caaacacgcc gcacgtgcaa
     2221 tcaataacgg aatgcggatt gatctcgtag atacgccggt gcgtgtaacg acgattgacc
     2281 ccggtctagt cgaaacggaa tttagcgtag tacgttttcg cggtgatgcc gaacgcgcca
     2341 agaaaccgta tgaaggcatc gcgccgctta gaggagagga tatagccgac ttgattttat
     2401 ttgcagcgac tcgcccgacg catgtcaata tcaacgaaat gatcgtgatg cccaaagcgc
     2461 aagctacatc cactttggta caccgcaaag tataacatta gttttgatcg agcagagtac
     2521 gaatcacttt ggataaggta tcaatcgaaa agggcttttc gataatggtg gacttcagat
     2581 tctgacgaat atcccgcacc acatccggcg ttgcgtatcc ggtagcaaaa aatatttttg
     2641 ccatcggatt gatcccgcac agagccgcat gtgtttgttt gccgtccatc tccggcatct
     2701 gtatatcgag catgacgacg tcgggttttt cctgctgata caaacgcacc gcttccattc
     2761 cgctttgtgc cgtaattaca cgatacccca gttcttccag tatgcgcgag catatgttca
     2821 gaatggcttt ttcatcatcc acgacaagca ccgtttcttt acccgtttcc aaaggttttt
     2881 cactctccgg cacgggtgct gactcgttac ttcgggatgc aggaagataa aatctaaatt
     2941 cggcgccttc gccttttttg gaatctacca tgatggttcc gccatgattg aggataatac
     3001 tttttgcaac agccaaaccc aggccggtgc cttggccttc cggtttggta gtataaaatg
     3061 cttcgaaaat attttgcaat gtccctgcat ccatacctac gcccgaatcg cggataatgc
     3121 attcgatata gacgccggcg cccggcaggt tgagcagctc ggctgattct ccgtcaaact
     3181 ctaccgaatg cgtacgtaca ttgatcaggc cgcctggctc gcccatcgcg tcccgcgcat
     3241 tgacaagaag attcatgatg acttgttgga tttgcgtttg atccacatcc atcgcaggaa
     3301 tacggtcatc cagacgtata tcaaaacgca cttgtttggg aaatgcgtgc gtcaccatcg
     3361 cttgcgtttc acggatcaga tcattgatat gaatccgcgt aatgcgtagc ggggtttgcc
     3421 gtgcatagac tagcaattgt ttgactattt cacccgctcg ccgcgccgat gcggcaatat
     3481 tttcggcata cgtttttacc atttcgggtg acgttgaaaa atttttgagc atctcggctg
     3541 tcggtaaaat cacggcgagc atattattca tatcgtgcgc tacaccgccg gctatacggc
     3601 ccaccgattc catcttttgc gcttgaataa gattttgttc gagccgccgc gattcggtaa
     3661 tatcttcaac agctacttca taatagagga aacgtcctcc gggatcatac accgcacgat
     3721 cgtgcaaacg cacctgtata atggcgccgt ctttttttct ccagaatact tccgcatacc
     3781 gtccaatgcc ctgggctcgc gtgttttcgt tgaattgcaa acgatcttca gcccgcacat
     3841 aaatatcacg attgagattg agccgcatga cttcgtcatg ggacttataa cccagcattc
     3901 gcactaatgc cggattgatc gtgataaaat ttccttcttc cgtggcctga taaatacctt
     3961 cggttatgtt ttcaaaaata gatcggtatt tattttctga cgcaatcaac gcctgttcca
     4021 ctttgcgccg ctccgtgaca tcgtgaatga tcgaaaaaag taatttgcgt tcgccgatca
     4081 ccaccggacc cgttcgtact tcgacctctt ttatttctcc gttggccaga cgatgctgaa
     4141 aatcaacata ggtttgctgc gcttccatgg cctgcgctat gcgctctctt acttcttttt
     4201 caggcaacac attgatatcc caaaccctta ggctttttaa ctgatcgacc gtatacccgt
     4261 aaaatgcggc agctgcagga ttagcatcca cgatacgccc gctttccggt tccacaagcc
     4321 atttgatggc cgtattgttt tcaaacattt gacgataccg tatttcacta tcggaaatca
     4381 tttttcgaga ttgcatacgt tccgttacat cgcgggaaaa tacgctgacg ccggtgatta
     4441 tatgttgcgc atcgcggagc ggataaaaac tgatatcgta ataatggctt atcccagcaa
     4501 attcaaacag tttttcgatg acaaaccgtt ctccctccag agcacgctga taataacttc
     4561 tccattcgtc ggctgtcgcc tttggcaaat attccaaaat ggccatgccg ggttgtaata
     4621 taacgccgta ggctgattca tacgccacgc gaaaggccga attgagaacc actacgcgca
     4681 gattccgatc gacggaataa atcgaatccg tcgtattttc gatgaccccc gacagattgt
     4741 attcgctttg gcgcaatgct tcttgtgcga aacgccgttc cgtaatgtcg tgatagttgg
     4801 taacgatagc ctggacaaac ggtacgtgaa acatattgat cgccgaaaac tccacccaac
     4861 gaatactgcc gtttttgtgc cgaagcggga ccgatagatg tacgcgccga ttttctcggc
     4921 ggcgaaccga ctcaaaaata cgctgaaccg tatccgcgtc actctgaata aaatcaaatg
     4981 cgctgcgccc cgaaatttcc catgtttcgt agcccagtaa ccgtttgatc gaagcgctgg
     5041 tgtagagtat ggtgccgcgc tgatctacca atgtgacaac atcgctgctg ttttcgatca
     5101 aagcgcgaaa acgttgttcg ctttcataga gttgttgctc acgccgcttg cgatcggaaa
     5161 tgtcgcgtac gataccgagc gctcttccgt cattcaatac ttttgcggaa atctccacat
     5221 aaaaaagggc gccgtttttt ctcttaaatc gttgctcgcc cagatacttt ccctcttttt
     5281 gcatcaaggc cagtgttgta tccagagtat gcgccgtatc gggatcaatc agatccaatg
     5341 gccccattgt aagaagttct tcacgggaat aaccggtcat cacacacgct ttttgattaa
     5401 cggagagata gcgccgatcc gatcccatga taaatacggc atcggatgaa gactccagca
     5461 acacatcata atcctgacta atacgctgaa tgcgccgtgc atccgccgca tgccaacgct
     5521 gcatgagcca agccatcgcc aataccgcaa acgtattgac gataatccaa aaaactatgg
     5581 tccaaaactg ttccggagct tcattgtacg gatggccaaa atccagcata taataaaggc
     5641 ggtataaaaa ctgaagtacg gcgctcacta aacccgaacg cagtcctccg agcagtacgg
     5701 aaaaaaatat caacgcctga agagcaatcg aaccgggacg ataattttgt atctgaccgg
     5761 cggccaattc taaaatcaca atagccacca atgtggcgac cggaccgatc gaacgacggt
     5821 aacgaaaaag aaattctgtc aacttcatga gaaacaagaa aggattaata taatggataa
     5881 cataaccaaa cgattaagat taagcaacac catcttgcaa ttcgcccgac gaattctcat
     5941 attgaaaaaa aacggaagca tagtatgacc cgcgcacaaa agttatatac gattctttgc
     6001 ggcattttta tttcggcatt gatcgtcgcc gaaatcaccg gcagtaaact cattcagcat
     6061 tccgtctccg aaaattgggt ttttatactc acgatgggta cgatcccgtt tccgattact
     6121 tttcttgtaa ccgatttgat caatgaatac tacgggaaac ccggtattcg tttcgtcacg
     6181 ctgcttggca tggtactgat cggctttgtg tatgttatct tgtttattga tatgcgtatt
     6241 cccgcggcgg cgatttcacc cgtatccgat gcacagttca atgccgtttt tggccagtcc
     6301 agccgcatta ttttaggttc ggttacggcg tatctcatcg gccaatttat tgatattcaa
     6361 atttttcata ccatccgtaa acataccggt gagaaatggc tttggctcag agctacgggt
     6421 tccacttttg tatcccaact cattgactcg tttgtagtac tattcattgc attcagcaat
     6481 actttgtcat tcgagcaaat tatgacgatc ggagcaacga attatattta caaatttgtc
     6541 atcgccatta tcctgacacc cgtgatttat ctgacccatg cgatgattga ccgctatctg
     6601 ggtgtctcgg aatcacacac aatgatcgca cagtccgtcg aaaaataaat gaatacggaa
     6661 ttcaacctat gcctgaaata ctttgccgcg tgatagactg ccacgttttt cgtcaaacac
     6721 aagaagggcc gcgttatctg gtgatgcaac gtgccaatga agtgatttac gccggttcgt
     6781 ggcgtatggt cggtggaaaa atagaaaacg gggaaaaagc gtgggaaacg gctttacggg
     6841 aattgagcga agaaaccggg cttacggcta tgcgtctttg ggccgtgcca tacatcaata
     6901 gtttctacga ggcttccaaa gatcgcgtca acatcatacc tgtttttgcg gcgtgcgtac
     6961 aaaacgataa cgtaacactt tcgcgtgaac atgacgcata ccgatgggca tcatatgatg
     7021 aggctttgac tttattaccg tggccggctc aaatcgaggg attgcgtatc gttcatgaat
     7081 acatcactac cggaaaaacg gttgccggat ttgtagaaat ccctttacgc gcgtagtttg
     7141 ggatccagag catcgcgtac tccgtcgccg acgatattaa aactcatcac ggtcaacacg
     7201 atcgctaaac cgggaaatgt cgaaatccac cacgcgtgca gcaaggcatc gcgtccatca
     7261 ctcaccatat taccccagct tgccgtaggc ggctgaacac ccacaccaag aaaactcaat
     7321 ccggcttcga ctaaaataat cgcgcctaaa cgcagcgtcg ccgcgacaac gaccggcgct
     7381 aaagtgttgg gaataatatg cacacgcatg atgcgtgcat gcgaataacc caatgcacgc
     7441 gccgccgtga taaattcact gttacgcaat accaacacct gacttcgtac gatacgcgat
     7501 acgcccatcc atccggtcaa tcctaatacg gcgatcatta gtaaaaccga attgccaaaa
     7561 agcgcaacga tcatgaggat gagaaataaa ttaggaaaag ccatcatgat atccaccccg
     7621 cgcatcagaa ttccgtcaat ccgatgacca aaataaccgg cgatcgtacc caccaatgtg
     7681 ccgagcgcca cggccatcac catggccagc ataccgatgg ccagcgaaat gcgtgtaccg
     7741 tagatcagtc ggctcagaat gtcacgccca aagcgatctg agcccaacca atacattctt
     7801 tgtgttagaa aaattatagg ctcagtacct attaaattcg aaatatccag tttctttttt
     7861 tgaattcctt gcgtataaaa aacttcgtta ccctgaagat gccattcatc aacaaacagt
     7921 aagggttctt ccgtttgtcg aaactgttga ttgatgcttt gaagtgtagc ggtccagtac
     7981 tctgaaaagt tttgtgcgga tggccgaggc ggcacgatat catttctttt aagccgcaag
     8041 gccgtaacgg agtgcatggg ttttatgtat ttagtaacga cgatatcttg ttgcgcattg
     8101 ggatcatacg gcgcatacca gggtgcaaat atggccataa gaacgagtac catcagaatg
     8161 cataaaaaac acatggcaaa gccattacgc cgaaacgcct gtccggcaag cctccacggt
     8221 gatgccgatg ttctctgttg agttatcaag cgttttttgt gataccaata aaatacgccg
     8281 atgatccatc ccgatgccgc cagagaagcc accgcatgct ccatgatttc cggtgaccac
     8341 agacgcgtaa gtgcctcaaa atttgtaacg atcaatattg gtacggtcac cagtgcatgc
     8401 caacctgtga gaaaggcctg ccatgaatag ataatcatca ctattggtaa caaccacgat
     8461 agaagcataa ccatagctga ggtacgatcg ccacgccgca tttcgtacaa acccggcaaa
     8521 ctccgtatcg aaacgagatt tcgaatccta ttattcgcca tgttcgaaca tgctccgcca
     8581 gtaatccatt tcacgctctg ataacgacgg atcgtttttc gcgctcagtt cgtcagaaac
     8641 cggtttattt ttaagcgtcg tcaatcgttg gaaaaaatct tcggagcgct cgactcggca
     8701 accgcaccct tgagcatacc acatcacttc gttatcggaa gacacgacgg tgatcgtacg
     8761 cgctttcgcc tgatcatcca cgaggcgttt gatcgcttca tcagcgcgat ttggaagatt
     8821 ggaaaatagt atgcgtacgc cggctttggc ctgtagcgac attttttttc cgtcaaatac
     8881 cactaccgca tcgtggtttg tcatatttct aaacgtagcg atatgcacca aaagcgcctg
     8941 acgcccttgc tctaaacttt ttatttcctc tcgtcgcaaa gcgtcaatac gtttgatcag
     9001 attgtatccg tcaaaaatat aaagcggttt ttgtgtcata cccaggcgtt cagtttttcg
     9061 agaatctgtc gtatttcatt ttcggtgtta taaaaatgcg gcgaaacgcg aatcgcgcca
     9121 tcgcggtacg atacggtaat tcccgattgc ataagctttt caaaaacgtt ttttgtagga
     9181 atgctctgct cgttgcgcga ctgaaacgat ataatccccg agcgatgagt ggacgtaaga
     9241 tcgctacgga taatccatcc ttgttcattc aaaccttgcg ctaaaagatc gatgagcgcc
     9301 gtaatatgat aaaaaatatt ttcaatgccg acttccatca aaagcgacac cgacgctttc
     9361 atgccgtaga tacccagcat attttctgtg gccaattcaa accggcgagc atcttccgga
     9421 aaatccaaag catattcacg aagattccac ggatgcttta ccgacagcca accgacctgt
     9481 tgaaccgtca agcgatcaat cagttgttcg ccgacatata cgaaacccaa tccgcccggc
     9541 gccatgagcc acttgtgacc gccattggat aaaaaatcca catgggtttt tgttacatcc
     9601 aaagggacgg ctccgacacc ctgaatcccg tccacgcaaa ataatattcc gcgagcatgg
     9661 catgctctgc ccagcgcttc caaatcagca cgaaatccat tatggtattg gacaaaacta
     9721 accgagacca ggcgcgtcgc cgcggtaaat cgcgaaacaa aatcatcggt cgtaaaacgc
     9781 cctgtaggtg tcggcacaaa ttcgacacgc acaccgcgat cttgcaattt taaaaaagga
     9841 tatacattgg aaggaaattc accctccgcg atcaggatat ggtcaccgcg ttcccacgac
     9901 aaaccatcag ccagaatgtt taatcccgcc gatgtgtttt tgacaaagcc gatacgcgct
     9961 ggtagggtgt gcagcatttg tgctaagata ccgcgcagac tttcgcgcgt ttccatccag
    10021 acgggaaaat tatcaacgtg cgtcgtagaa gaaaccgtca aaagttcgtc aatggccgat
    10081 ttcacgcgtg tagataccgg cgaacaggct gcatgattga catacacccc atgttgagtg
    10141 gacggaaaaa gcgcccgcca ttggttgagt ttatttttca tttttttata aaaatatccc
    10201 ttagtgccgc atgcagttca tcaaacgtaa accgaaaacc ggcattttgt aattttaccg
    10261 gaagagcttt ctgaccggct aataaggaat cggaaaattc tccgacaact atttttaaaa
    10321 caaaaccggg aacaggcgcc caactgggtc ggcgcagcac tgaaccgagt gtttgagaaa
    10381 attgtttcat tgtcacaggc ataggggctg tcccattaac cggaccgcgg atggtttttt
    10441 catttaaaat aaaaagcacc gctcgcacaa gatcgtcacg gtgaatccac ggcaaccatt
    10501 gtcggccgga gccaagcggg ccgcctacaa aaagtttaaa cggtaaaacc attttttcta
    10561 atgcaccgcc accttcttca agcacgatgc cgatgcgcag acacaccacg cgtattccca
    10621 atgtttccgc ttgtaagact tcgttttccc atgcagcaca cgtgcgacct aaaaaatcat
    10681 cggatgcgtg gcgggtttcg tccacatcgc cttccggaac atcgccgtaa taacccaccg
    10741 cgctcgcatt gatccaggtt ttgggttttt gttgcgctac gtggcacgct ttcatcaata
    10801 tgcgtgttga taatatccga ctatcggcta ttattttttt ataagatgcg tcccaccgtt
    10861 ttccggcaat actagctccg gtcaaattaa ttatggcgtc cgcttcattg atgtcggaaa
    10921 accaatcccc cgaagaaaca gcatcccaga aaacggcttt gacagtatcg ccccactgat
    10981 ttcgaagcac atccggtcgg cgtgagagaa tgataaggtg atgtttttct tctaataatg
    11041 cctttatcaa agcatttccg ataaatccgg tcgcgccggc cagtacgatt ttcatttaaa
    11101 atgcatctaa gattatgaat ttgaatagct caatgtatta gattcatctc ttaatttgca
    11161 catttttaat tctaaaggaa aaaaattaat taattttatt caaaataatt acttttaaaa
    11221 gtcaaacaaa ctaacttatt attaatcaac gttttgtgaa aaacaagctc cgtattatta
    11281 tgtattttta attttttttt ggattactgc aatatttttc ttgcgcagta acctgtgata
    11341 tattatattg aaagcgtgat cgttgatcac attgttcttg aaacccccac ggaggttacc
    11401 ccctaacctc tggctacgat agagacccag ggttgtttcc ctcatccctg ggttttttat
    11461 tttcattttt ttctactcta aaattcataa attaatttgg tactaaatta cttttttttc
    11521 gtaccataat aatggctcat taccccacaa tacatcgttt gcgcaggtga ttatgaaatc
    11581 gtatcgcgta ttttttttat tactgatttt gttcgttggt atcggaatag gactgattct
    11641 tagttttaat atcggaaagc cgcgtctttc tgctcaaaat tcttcgacag ccgattcttt
    11701 cccgatcgtt catatttcac aagcgttggc atccgagctt gccgcaactt ctttttcagg
    11761 gatcgcccgg ctttcgacac cggcggttgt aactattaca agcgaaaaaa aacaatcttc
    11821 atcggacgaa gaaacacaat tccgctttca ccgtgatatg cccgagcaag gtgtcggttc
    11881 cggcgttatt gtttccgccg atggctatat cgtaaccaac aatcacgtga ttgatcaagc
    11941 cgaacgtatc gtagtgcgtc tagccgatcg ccgctctttt gaagccacac ttttgggaac
    12001 cgatccactg acggacatcg ccctcattcg cattgatgcg acaggtttgc ctaccatcac
    12061 gtttggtaat tcggacatga tccaagtcgg cgactgggtc atcgctgtcg gctcgccgct
    12121 cagtcttaat tcgaccgtaa ccgcaggtat cgtcagtgca acgaatcggc aaatcgaaat
    12181 catcggtgat caatatggcg ttgaaaattt tattcaaacc gacgccgtta tcaatcccgg
    12241 taacagtggc ggtgcgttgc tcaatatgcg gggtgaattg atcggcatta atacggctat
    12301 agccagccga accggcatgt atcagggata cggcttcgcc gtcccatcca atattgtgcg
    12361 taatgccgtt caggacttat cgcggtacgg gcgcgtcgtg cgcggctata ttggtgtcgg
    12421 catcaaagat gtggatgcta tatatgccaa agccatcggg ctagataaag cccgcggtgt
    12481 attggtagag tcgcttacgg aaaaaggcgc cgcacggcac agcggcgtgg aagaaggcga
    12541 cgtgattttg caaattgacg atatgccgct ttttcagcgc aacgaactgc aagcctacgt
    12601 cgcactccac aagcctggcg agagtgtgcg attacagata tggcgcgacg ggcgaaccgt
    12661 cgaaaagaaa gtcatgctgc gcaatcccga cggcaatacc gatataccag cagaaaaaat
    12721 acaacggcaa aacgatgcac atatcggttc agaatcatgg ggttacgggc ttggatttga
    12781 agtggagaac ttaagcgatc aagggatgcg cggtgtgatg atttccaaaa gctcatggcg
    12841 tgccaaggct caaaatattt acgaaggcat gatcctgacc cgcatccaaa aaacggatat
    12901 caatgacgtt tccgtttttg aaaaagcagc acgggaattg aaaaatggtg aagcggtaat
    12961 tatccaatta gccgatcgtc gcaacccggc caatcatatt cttacggccg tagaggtacg
    13021 ccgttaaaac caatcgcttg aacaaaatat cgaaccatta tccgatctga agtttcatac
    13081 cgtcgtcggt ccatgtaccg gtattgggtg acgaatccgt cggtttaatg atcgcactcc
    13141 cgatcgtaag taatccgaca cggcctacaa acatcgtgac aacaatgagt attttggaag
    13201 ccggcgtcat caacatcgta attccggccg aaagtcctac ggtaccaaac gcacttacaa
    13261 cttcaaaagc catttgcgtg agtgtaaagc gcgtttccaa aacggtcatc aaaagtatca
    13321 cgctcaatac aaaggtagaa ctcaaaacgt aaagcttcac ggcattgcct accgatcctt
    13381 tgctgaccac atagcggaac aaagagacac gattcatacc gcgaaacgaa ctgcgaatat
    13441 atccgagcat cgtggcaaac gtaggaattt taatgccgcc ggccgtaccc tggggtgctg
    13501 caccgataaa catcagcgaa ataaatacca tcaaagtacg gatttgaaga agtccgatat
    13561 ctacggtatt aaatccggct gtacgtgtgg acaccgagtg aaaaaatgaa atcaaccatt
    13621 tttcgccggc atcgtactga cccagtgtat gcggatcatc ccactcgtaa gccgccataa
    13681 gtaccgtacc gatcgccaga agaaaaactg tcccccatat tacgattttc atttgcagag
    13741 ggcttgccac accggctaat tcttcacgta attttttgga acgtaaatat aaccgatcaa
    13801 caaaattata aaatggttcc accagataca acttgtccgg gcgtcgcatg gcgatcatct
    13861 ccatcaatgc ttcgctgaaa ttgagcattt tgtgaagcat cagcaggaaa aaactttcaa
    13921 tgccgagaag aacgggataa ccgataccgc ccaatacgat gagtccggca ataaccatat
    13981 tcacagatac atccgaacga aatggcatca ggttatccga atataagccg aaacccgcat
    14041 tattgaatgc cgagatcgta tgaaaaaccg agaaccataa cggtgtaatg gggccatcgg
    14101 ctaaacgatc cttcgtcgcc agataaagca ataagcaccc cagtccttcg atcgccaaag
    14161 tgattttggc cgtcgaagcc aaaacttgaa aaatcgtacg atgctcacct tcgacatcca
    14221 tcacggaacc taatgtttgt gctgttcccg gagaaaggtt acctgttcta ccaatccaca
    14281 aaagtcgaaa tgccgtcacc acaccaaagc caccgacttg tatcagaacc atcgttatga
    14341 cttgaccggc aaaagtgaat ttgctgaagt caaccgatgt cagaccggtt acacaaatcg
    14401 cggacgtcga aataaacagc gcatccacgt aggatatcac attttcgccg cgattttcga
    14461 tcagaaataa caaaactgag ccgatcaaaa tcgccatggc aaaaccgatg atggcaatct
    14521 cggctgccga atacttgcgg atactaaatt caatccgact ttctattcgg tttatttcgt
    14581 cttgtaaaga accggttttt gtttgagagt cgttggattg gggaggttca tcagatccgg
    14641 attgggatac cgaatccggt ttcgaagctt cagaagtaga gttttccata tatgtatttt
    14701 ggtccgaccg taaacaaacg atgtgtttgt ttttggtggg gagcaatata caccgagctc
    14761 tattgcaatg tcaataaatt tgttacgaat acaagtaagg cttcatacca aacaagtggt
    14821 agtaatttta agaacatttt tttaaatgtg agtcaatccg tatgatatgg gtgaccaacc
    14881 agacttttac caacgcctgg atttcgcgga gtaattgtat cgagtcgccg tcatgttgat
    14941 atttatccgc aattttttca tacgcttcga gaaatttttt atgagaatgc tcattatatt
    15001 cggctaccgg gcatttgcgt tcatgcatac acttctcttc gtacttaaaa tgatcgcgga
    15061 tgtacgcgct cagaaaatct atagcctgac tgaccagctc gtcactacgc ccgtttgtga
    15121 cgccttcctc caaatcggtc agaaaatgaa aaatattttt atgctgatcg tctatttctt
    15181 tatcaccgat caggtattca tccttccaaa taaaagacat aaattttttc tcactatagg
    15241 taggtcaaaa gaaccgactt aatttgcaag gctacagaac tataatcaag agataatgcc
    15301 aaaattggtg attttgagaa aacaacaaac tacttatgat ttaatcagat taaaggcatt
    15361 atgccctttt aagaaaagat ccgtcattta agcctataaa tgaccatttt tgttacttag
    15421 cgtatagcgc ccaaaggcct tctagtgaga cagattatga aaaacgcatt aactcgttaa
    15481 accgattcca tccggttcat gattcatcga aatctccaga aacgatcact cgattacgac
    15541 cttgcgtttt agcccgatac aacgctgtat ctgcccgtgc aatgagttga tcaaaagttt
    15601 tatcgtcagg ttggcattca gatataccaa tgctaactgt ataacgaaaa ggaataccgg
    15661 aatcgctcac ttgagaacac tcctcgacac ctatacggat acgctctgca agcataacgg
    15721 ccatgttttt ttccgtgtgc ggcattaata cagcaaactc ctcgccacca agtcgggcaa
    15781 ccgtatcggt ttgccgtgaa aatttttcac atgtgtttgc taacattcga atcacttgat
    15841 cgcccgtagg atgtccataa gtatcgttaa tggatttaaa gtgatcaata tccatcataa
    15901 gtaaagctaa agaatgccca atacgtctgt gccgaaaaaa ttcctgttca agtaatggca
    15961 taaatccccg cctgtttaca gctccagtca taggatctgt ttttgttgcc aactctaaag
    16021 aaatttcaaa ttcataacgt ttacggtaat tgtgcgttgc agcaatgtgc cctaaaatgg
    16081 ttaaaacagc agctggccat atcagagtgg caaaattaac atgttgaaat ccgccaaaat
    16141 aaccaaaaaa tgcaaagaga tgaggaaatg cagtgatagc aatggcataa gggattgtca
    16201 atcgtaaggg taacccaaga aagccgatta atgaaaccaa aaaatagaac atgaatcctc
    16261 caatcccata aatcacgccg gattcgagtc tttcaaatat ctgaatcaaa aatacctcgg
    16321 ttacaagcat agctataact gagaatatcc caaccaatcg atagtctttt atccgataca
    16381 ttaccattgc gaccccgata aaaatcggga agaacgccag acgtaacaaa aaagtttgtt
    16441 cagcgccaac aggatcgcga aagtaatccc attcccacaa accggcggcg aatggagcgc
    16501 cgaggataaa cataacgaca ctcatattac gaaagtcatc catacgtttt tgtaaatagt
    16561 cattgtttac tccgatttct aaaaattcgg aacaaatgcg tttccaattc atcgattaat
    16621 ctttcctatt tagagttaag cggcttactt ttggatcacg tcaccggcaa ataattcttt
    16681 tttaatgcgc gatccccatg gacaatacgt tcctactgtg ctcaaccact catcaagatg
    16741 ttcttcgaca tattcgggcg aaccatcgca cagctccata gctgtttcag caaaatgcca
    16801 ggcgttcgtc ttaaaatgcc aattccaatc ataattgtag tcttcattgc ctctatcaat
    16861 agggccaaca atcatcatat tgcgatctgt taacggtaat agcaacagac tatccgcaac
    16921 actctgaata acagaatccg atgtgcgaac aataaaaaaa tcgttcccaa ctgtagcaat
    16981 cagaaaatat ctaaactcat catccggtat cttaatgaca tcgctgcctt tgcaggaaca
    17041 ggatagagcg ccgaaagtta gcaacacaat caaaaatatg atcaacgatt tgtaacaaat
    17101 aaaaaatttg gcatttaaaa ctttcatatg tgtatttcct taaaaaaact tttgcatggg
    17161 attcaaatat tgaaaatcaa cgtttaaaca tgtacggccg taacaataaa tcggatgatg
    17221 cttgttactc aacatatcaa tcggaaacga tgaatttcaa tatcaattaa aataggaccc
    17281 ggcaacgccg ttctccacgg cgtttgcggt gttacctttc tgcgcgaagg atagatgaga
    17341 cctgtatctt cattatgcgc aaggatggta tgcgttgctc gggcatagcg ccctataatg
    17401 tccactgtgt acaaatgttc acaaagcaac ccggtgtcct tatcaaatcg aaattcctga
    17461 tttttggaat gcgtcggtat actggttggg aagaccgctt tcacgatacc ctgctcggtt
    17521 tcgctccaaa gaatctcctc attcattaat aagctaggca gtgtcaaata attccacatt
    17581 gcataattcg caaagtacga catttccaaa tcatcccacc gaaacgcatg ctttccaaac
    17641 gggaaatacc ggcggataga attacgagtt tctaatactt taccggatac gtcttccagc
    17701 cacacatctt cactgtcaag cctaccccga atgctcggat ctctaccaat aggaataata
    17761 gatgaaaccg ggcgagcaat ttccatttgg accaaagcgt gttcaaagaa gggcctttgc
    17821 ttgagagtaa aagccagccc ccgcacactg atttcagctt cgatccgttt agcctttttc
    17881 caacacgcct ctccgccgta cgcccggatt gctttattta tagtagattc actcattatt
    17941 tagccaactt tctgattatc aatgcccccc ccaatacttc taaaaggctc catggaatgg
    18001 caaaatataa tattcccgac ggaagcactc ccatattacc gataactatc cacatgagaa
    18061 caaatcctcc gaaccaagca attaaccaag cctgaaataa tgaaaaccgg gaagttaata
    18121 cataaatcaa tatggccaag aagaccgacc acacacccca aatcgcgcca ttgatcggat
    18181 cggatggaaa ctttagcccg aggcttaggt agtgatttgt ccaatacgtt ttgaaaaata
    18241 tttcgttacg caaaaattct gacacaccaa tccatagcga agccaaaaga accggaaccc
    18301 cttctttttt tagccaatcc atacagcgcc tttccaaatt aaaccatact gtgttactct
    18361 tagacgaacg acgacggtaa aacggtcgga gaagcaacga ggttgccctt aattggcgga
    18421 ttatcaatct ataaaaaagc ttcaaaagga tacaaaaaaa caccttgtcg gcaacaaggt
    18481 gttttacgag cccagtaaaa taaaaatgaa caatcactca tgctttcagt tgttttatcg
    18541 ccgtcggcca atcttccaaa tgatgaaccg ctgcaatttg accgcctacc atcgtatgta
    18601 tatcaatagt cattattttg aaagattttg ttccgtccgt aggcataccc ataaaatcac
    18661 cattgggcgt cccggaagcc atactacgca cgacgtactt atttccctcg ttaataattt
    18721 cctgtatttc ccatttcaga tcaggaatca atttccaaaa aaaaacaagt tgcatgatga
    18781 gttgcatttt atttttcgac tcaaccgatc caagcgatac aaactgatcg gccaaaatag
    18841 agttcaatat ctcggcggga ttagtctcgt tattgactgt caacgctttg ctgtaaaaca
    18901 tttgcaattg ttttccatta ctgtccatgg tcgtgaaatc ctttcggtga ttgatttact
    18961 gtttctacgc ccttaatatg cgtttacaga aaaggttgaa caatggacaa aacggattta
    19021 cggtggtact tttgagacaa cggcatttta tttgaaagtt tcacgaaact ctagaggcgt
    19081 aatattcgca aaccttttga aatagcgtac gaagtaggag ggatcgtcaa aacctagatg
    19141 atgagcgatt tctttaacgg atctattggt atacgccaag aggcgtttag cttccagcaa
    19201 caggcgttca tgcactatgg ccttagggga cttccccaaa atacgcatgg tcaagcgctc
    19261 cagttttttt aaacctatac ccatttcgtc cgcatattcg aatacggttt tatgctctgt
    19321 aaatcggttt tccaatagct ctctgaagcg caccacctca tcccaatccg cgtcttttct
    19381 tttttcacga aacccttggt cgcggagcgt acgttcggct ataataagga aattttgtaa
    19441 aagatttttt accaataccg gctccgaacg catggcattg ctatcgcttt cctcgcgaat
    19501 atcctcgcat atggtcgaaa acttcgcaaa caaaacctta cttaaaggga tagtaaatat
    19561 ttcgtataag ctatcaaaaa gtggcgtatt ttttaaaaaa gaaacatccg attcggaaac
    19621 tgaaaataca tgctccgtaa aaataatgat gcgcccttgc agtttaatac ttgggtcaaa
    19681 ggcatgaact ttccctttgg caataaaaag aagggaataa ggttggattt ttattttttt
    19741 aaaatccaca taatgtgtac atgcgttttc aacaagaaaa acatggtgga aatcggtacg
    19801 gtgcggtttg gataaaacaa cgcggccgat tctttttagt ttttctattg ctacaacttc
    19861 aacttccagt cgagaatcgt ttctataaaa acgacgcgtt tcgattttct ctttcataac
    19921 aattttattt atttcaacaa aatcgcataa ataaattgta tttttttgac tcgttcaaag
    19981 cttttttttc attcaaagcc tttggtatga agcaaaaccg ctatcatcaa ccagtaaact
    20041 aaatctgtgt ttcagccctc ccctttttat ccggcgacgc gggccccatc tccccgatcg
    20101 caatgcgcgt acactgcatt ataatgtcct gtaaatcaga accctccgat cgtaacggat
    20161 caatttggga tacgagtttt gcgcgaagct catagagttt ttcacgatcc ggggtgttcg
    20221 ctcgccgtat caattcttca ttctgtaagg ttttgcgatg atccgactcg gggttaaaaa
    20281 aatcagccaa ctctgtacac tgatagcata cgccgttttt attcaccagt gcacagcgcg
    20341 aatcgaaaat ggtctgcatt tttttccggg catcaaagag ccgatgtttg atcacccctt
    20401 cagataaccc gagtatcata ccgatttctt ttcgtgaaaa gtcatatata tctttcagaa
    20461 tcaaagtaat ttgttcatca atcggtaatg tttttgcgat acaagtaaaa caaaaatcaa
    20521 tatgttcgcg cacgtggtaa gcgccatgcg gcgaatagtg atgcgcgtcg aggaaccgct
    20581 ggcgcatcat cgtggaggaa gaagcgtagg ctttggcttg atcttgcgca tcgtgcttcc
    20641 agcgtttttt gtcacgcaac aaatcatacg caagattggt cgcaatttga aagacccacg
    20701 ttttaaaaga cgactctcct ttaaatgttg atattttatc aaacgctttg acaaaagtat
    20761 cttgcgaaat atcctccaca tcgtgacgat ctgtgacaag ccgatataga tacgacctta
    20821 gcgtatcttg aaattccaaa aaaagttttt gaaacgcctg tatatctccg ttgacggctt
    20881 gttttaaata ttcatttttc atacctgaat aacctatcga agtttgtacg acatatctga
    20941 acacaggcga catgaccggc acccacacga caatattata cttttatata acatttgcag
    21001 ctccaaaatc agcgtcaata acgtggctat taaatatact accggctggt gtcgccagaa
    21061 aaacggacag atttccgata tctttggggc aaggcccgta gcctaataat gcattacttg
    21121 aagttttttt gtccacttcg atcggaataa cgcccgttgt tcgttcgatc agcgctgtcg
    21181 tttcacgtat cgtgcgtgtg gtgggcatac ctgccgcgtt gatgcaaatc accttgatcc
    21241 ccgatgaacc aaattcggcc gctagtacac ggctcatgcc ttcgacggcg gagcaagctg
    21301 cggtgatacc ggccatacat gggattttga ggcgtgacaa acttgcagat agggtaagaa
    21361 tagtaccttt agaaccaagg cggcgcataa tttttgcggc tgtacgggac gtaagaaatt
    21421 gggaaccggc attaatttga agagttttca tgaacaagct aaacggcaaa tcggcgctgg
    21481 gtgtaccgta tcccaaatcc actggtcgtg cgccgatacc gttaaatacg atatctattt
    21541 ttccagtcag gttcgcgata agctcgatgt aagcgatgat ttcattttcg gattcagcat
    21601 ctacctgatg cgccatcgct ttacctccac tactaagaat tcgatctgca atctcctgaa
    21661 ccgcagcaag gcgtcggcct gaaagaaaaa cgtgcgcccc ttccatagcc atagcctggg
    21721 cgacttccga tgcgatagca cctccggctg caaatataac ggcaacttta ccttcgatta
    21781 aattcataag cctccggtaa tttatgtgtt atattttaga cgtagggcaa ccataaaacg
    21841 gtcggaatgg ctatgaaaaa ataaaatata cgattaaatt ataccacgtt agtataaact
    21901 atttcactgc actaactgca aataaaaata catcaaaata caaacggcta aaaattcagc
    21961 cgtaaaaatg gccaacatcg tttttttgta ttcttgcttt tccataccgt tcattcttta
    22021 taattaatta ctcagattta aaattaacgg cggagcggtt tgacgcgctc caccgtcttt
    22081 cactaaacca agggggaatt agttaattag ttgatcactt tacgatacgg taacgctacg
    22141 atcagtgagt tgtaaggtgc cgcatccgca tcggattcgt acacagtgct gtgagtaacg
    22201 aaatccaaag cgccgacatg tacaccggca aattcggtcg gacctacaat ccagcgaacc
    22261 gtataaatac cgtcaccggt aacacgatcg ccatatgtac cttcatcgtt aaaaagatgt
    22321 ttgacgcgca tacctgcaat tttggcatta cggtttttac cgcgatgtat ccatcccatc
    22381 ggcgcatcgt cagcattgct attcgtaata ccgacttcta cggtcaccgt atcgccctga
    22441 ctcacttgcg gtaaaccacc gcgttcgatg aagtacgcga gtggatcggt aaatccgaaa
    22501 tccgttacac cggctgcact gtgtatggtt acattgacgc tgctcagact catcgttccg
    22561 tttttggata taccaaatcc ggccgacact tcgctcacaa gccagcggcg atctatctcc
    22621 ggatcggtat tgcgtacgaa acgaacgata cgtttcattt catgttcgag cggcttagta
    22681 tatttgacca atccttcgcc gtccggattt tccgcacgaa tcacaaaatt acctttcata
    22741 agaaatgtga tttccgcttc ggcgctcatc gtatcttcgg caaccgttat ctggatatct
    22801 ctacggctca tacgtacaac acgaccatac cctttgatcg aatcaatagg cgacgcggac
    22861 ttagataata cgaccgcctc accatcatag gacttggaat accccgttgc atccgattca
    22921 cccaaaatat ctacgccgat tatttcatcc gcatcttcga ttatctgatt ctgaatttcc
    22981 tcttcatcaa ctgtcagatt ggaactatct ttttcagaac acccgataac cagtaaaaaa
    23041 ttgaccatac tcattacgat caaccatcgg cttgcattcc aatacttttt catacacttc
    23101 actcctttat gtttgtggta aataacttct aacattttat gacccgctgc ataatattaa
    23161 aacaatttat atgccatttt ttaaatattt gatatttaat gttataatga ttaaaaaagg
    23221 caatggttga cgaaaaaacc gtcgtttttt taaattagtc aaaacaaata actttcaatt
    23281 tggttaaagg aaattttgac atagataaaa gacaattagt catatattaa gatgacattt
    23341 tagcactcat gatttatgag tgctaacaag tagaatgcaa attaaataaa aacaaaaaac
    23401 ttgaatttag gcacaaacct tgtatatttg cggccaacga attcaagcta taaccattat
    23461 ctaataatag taacttctta actcatactt cataaacagg agattctctc atgaaactga
    23521 agcccttagc ggatcgcgtc atcgtggaac cgatggaagc cgaagaaaaa acagcatccg
    23581 gtcttatcat tccggattca gccaaagaaa aaccgcaagt cggtaaagtc gttgccgcag
    23641 gtcccggcaa gaaaaaagac gaacccctca ccgttaaaac cggcgacatg gtgctttacg
    23701 gaaaatactc cggtactgaa atcacctatg aaaacaaaaa atatctgatc atgagcgagt
    23761 cggatatcct cgccgttcaa ggttaatcta attttagaat actaaccgat aacgataaca
    23821 atcttacagg agatacatca acatggctgc aaaagaaatc gtatttgatg ttgatgcccg
    23881 ttcaaaactg aagcgtggcg ttgacgtatt ggccgatgcg gtaaaggtga ccctcggtcc
    23941 ccaaggccgt aacgtgctga ttgataaaaa atttggcgcg ccgaccgtta ctaaagacgg
    24001 tgtgactgtt gccaaagaaa tcgaacttga agatcccatc gaaaacatgg gcgctcaaat
    24061 ggtgcgcgaa gtcgcttcca aaaccagcga tgtcgccggt gacggtacta cgaccgcgac
    24121 cgtattagct caatccatcg tgcgcgaagg tttcaaaaac gtaaccgccg gcgctaatcc
    24181 gatggaattg aaaaaaggta ttgattcggc tgtcaaagtc gtcgtcgctg aactcgcgaa
    24241 aatgaccaaa aaagtcgaag gtaaagaaca aatcgcgcaa gtcgctgcga tctccgctaa
    24301 caacgacaat gaaatcggta aattgctcgc cgatgcaatg gaaaaagtcg gtaaagacgg
    24361 tgtgatcacc gtcgaagaag ccaaaggtta tgagactatt ttggaaacgg tcgaaggtat
    24421 gcagtttgac cgcggcttct tatcccctta tttcatcacc gatgccgatt cccaagaagc
    24481 tgttctcgaa gatcctttta tcctgatcta tgacaaaaaa atcagcgtaa tgaaagatct
    24541 gcttcctatt ctcgaaaaaa cagttcagat gggcaaaggt cttgtgatca ttgcggaaga
    24601 tgtggaaaac gaagcgttgg ctactctcgt cgtgaacaaa ctgcgcggca ctttgcgcgt
    24661 agccgccgtc aaagcgcccg gtttcggcga tcgtcgtaaa gcgatgctcg aagatatcgc
    24721 aatcctcacc ggcggtaccg tgatcagcga agatgcgggt tataaactcg aaagcgctaa
    24781 agtcgaatac ctcggccgcg ctaaaaaagt gatcattgac aaagacaaca ccacgatcgt
    24841 cgaaggcgtc ggcaaaaaag aaaccatcaa aggacgtatc gctcagatca aaacacagat
    24901 cgaaaatacc agcagcgatt atgataaaga aaaacttcag gaacgtttgg ctaagttagc
    24961 cggtggcgtt gccgtgatcc gtatcggcgc ggcttcggaa atcgaaatga aggaaaagaa
    25021 agcccgcgta gaagacgcat tgcacgctac ccgtgctgcg gtcgaagaag gtattgtggc
    25081 cggcggcggc gtggctctgc tccgcgctca aaaagccctc gaaccgggcc gcggcggtat
    25141 caatgatacc ctgctcaaag gcgacaccgg tctcggcgta tccatcgtca aacgcgccct
    25201 cgaagagccg atccgtcaga tcgtcgaaaa ctgcggtatc gaagcttcga tcgtggtcca
    25261 aaaagtccgc gaaggcaacg accacttcgg tttcaacgcg ttgaccgaac agtacgaaga
    25321 tttactcaaa gccggcgtta ttgacccgac caaagtaacc cgtacggctt tggaaaatgc
    25381 cgcttccgta tccggtatgc tccttactac cgaagcgacc gtgtatgaga agaaagaaaa
    25441 caaacccgct atgcctccga tgcctccggg cggcggtatg ggcgatatgt attaatcgtc
    25501 agcttaatac gtctcccaag ttccgcaggc gaaaacggcc ggcggacatc aaatataaaa
    25561 tccctgttct gcttcatgcg gaacggggat tttttttgga aagcatgcaa caatttgccg
    25621 ccggtaacgt tcttatccga tccgtaaaac cgtgcatttt atcataccca atgtcaaaac
    25681 gtttttgtag tattacaccg tttccacgtc gttaaaaatg ttttttacat atccatcact
    25741 atctcagcga ggttaatgtt atgaaacgaa atctctggtt atgcttatgg tttgccctct
    25801 ttacgctttc atcggcacaa gcgcaacaag agtcgcgctt aatgcgtttc ccggcgacgg
    25861 atggacagca aatcgtgttc acctatgccg gtgatttata caccgtccct gtcaacggcg
    25921 gattggcacg caaacttacc tccgatgcca atggttacga aatgttcgca cgcttttctc
    25981 cgaacggtca atggctggct ttttccggcc aatacgacgg caacaccgaa gtttatgtaa
    26041 tgccttccgg cggcggtgtt cccaaacgtc tgaccatcac cgccaccctc ggacgcgatg
    26101 acgttgcgga tcgtatgggc cccagtacga tcgtgatggc atggaaaaat acatccgaag
    26161 aaattctatt ccgctcccgc atgcgttctt tcaacgattg gaacggtgaa ctttttagcg
    26221 tcaatctgca aggtgacgcc ccatctcaaa tgcccgtacc gcgcggcgga tggatgtcct
    26281 tttctccgga tgataaaaaa atggcgtaca accgcatctt ccgcgaattc cgtacctgga
    26341 aacgctatcg cggcggtatg gccgatgatg tctggattta tgactttgct tccaaaacgg
    26401 tcgagaacat tactaataat aatgcctccg atctttaccc gatgtgggtc ggcaataaaa
    26461 tctacttcgt ttccgaccgc gacgcccgct cccgatttaa cttgtacagc tatgatatta
    26521 catccaaaac gacaactcaa cttacaacgt tcacggattt tgatatcaag tttccttcgc
    26581 tcggcaaaga cgccatcgta ttcgaaaacg gcggctacat ttatcgtttt gatttgaaaa
    26641 ccgaaaaagc aacaaaagta ccgatctcga ttcaggaaga tttcgcatcg ggccgcaatg
    26701 cgcttcttca ggtcggcaaa aatgtaacca attttgaaat ttcaccggat ggcaaacgtg
    26761 ctttattagg agcacacggt gaaattttta ccgtgccgtc caaagccggt atcacgcgta
    26821 atctgacgca gacttccggt acgcacgaac gcgcgtccaa atggtcgccc gacggcaaat
    26881 ggatcgcgtt catcagtgat gcatccggcg aaaatgaaat ctacatcatg ccgcaggacg
    26941 gcagcgccaa accgacacag atcaccaaaa atgcggacac ctattactat acgctgtact
    27001 ggtctccgga cagcaaaaaa attctctggg cggataaaaa actccgtttg cgttatgtgg
    27061 atgtagctac caaaaatgta accgaagtgg atcaggcgac gttttgggaa ttttcggaat
    27121 atacgtggtc gcccgacagc aaatggatcg catacacacg cccggaagat gccggtatga
    27181 gccgtgttat gttgtattct ctggatcaaa aaaaatcaac ggaaattacc gacggttggt
    27241 acgaatccgg ttcgccggct ttcagccatg acggcaaata tctgattttc ggttccaatc
    27301 gcgattttaa tccggtttac agttggacgg aatggaatca ttcctatcag gacatgacgc
    27361 gcatctatat ggtcactttg tccaaagaaa cggattcgcc gttcaaaccg aaaaatgacg
    27421 aagtcgagat caaaaaggac gaccaaccta aagccgacga taaaaccaaa aaagatgacg
    27481 ccggcaaaaa agcggatgct tcagtaaaaa tagatttgga tggcatcaaa caacgcctga
    27541 tcgtattgcc ggtcagcgca tccaactatt ataatctggc aggcgtcgac gataagattt
    27601 attacagccg ccaaggcagc aaagacgaga aaccgctgct tttcgtgttt gacctcaaag
    27661 aacaaaaaga aaccgaactc ggatcattta acgggtatga aatttccgct gataataaaa
    27721 agatgctcgt cggccaaagc ggttcatttg ccatcgtcga tctgcccaaa ggaaaaatcg
    27781 aatttaaaga caagcttgac ctcggcaata tggagatgcg cgttaactat cgcgaagaat
    27841 ggatgcaaat cttcaacgag tgctggcggc aaatgcgcga ctttttgtat gacccggctc
    27901 tgcacggcgt tgattgggaa ggcgttcgca aaaaatatgc accgcttgta gaacatgtca
    27961 accatcgcaa tgatctgacc tatatcatcg gcgaagtcat cggcgaactc aatatcggac
    28021 atgcttatgt cggcggcggt gaacgcaaac aaccgcgccg tatcaatctc ggcttgctcg
    28081 gcgcagaaat cgaacgtgat cccgcaacga aatattatcg tatcgcacgc atcctgcccg
    28141 gtgaaaactg gcggaaagaa gtgcgttcgc cgctcaccga agtcggcgta aatgtaaaca
    28201 acggtgatta tatcatcgcg atcaacggtc agtcggtcgc cggagtcaat aatatcaacg
    28261 aacttctgat caataccgcc gacaaaacaa ccctgctgaa agttaactcc aaaccatcgg
    28321 aacaaggtgc gcgcgacgtc gccgtgaccc ctatcgctga cgaagcggac ttatactatt
    28381 atcaatgggt acaaaccaat atcgaaaaag taaacaaagc gacgaacggc gaagtcggat
    28441 atatccacgt acctgacatg ggcgtcaacg ggctcaacga atttgtaaaa cacttttacc
    28501 cgcagcttcg caaaaaagcg ctgatcatag acgtacgcgg caacggcggc ggcaacgtat
    28561 cgccgatgct catcgaacgt ctgcgccgtg aaatcgctat ggtggatgcg ccacgtaatc
    28621 tttcatcccg cccggatcct tttgatatga tggtcggtcc gatggtttgc ctcctcaatg
    28681 agttttccgc atcggacggc gatcttttcc cctaccgttt ccgcaaaatg aaactcggta
    28741 aactcatcgg caaacgcagc tggggcggtg tcgtcggtat tcgcggctca ctgccgctcg
    28801 tagacggcgg ttatctgaat cgcccggaat tcggttcata tgataccgac ggcaaagaat
    28861 ggatcatcga aggacacggc gtggatcccg atatcgtcat agataatgac ccggcacgtg
    28921 aattcgccgg catagacgac cagcttacca aaggcatcga ggtcatcatg gaagaattga
    28981 aaaccaaaaa acgcgagtta cccaaaaaac cggaatatcc gaaaaagtaa ttttttcata
    29041 acctaaacca atggaccaaa ccatgaaaca ttttctcatc acggctctgc tgatcacgat
    29101 gatcgcatgc aaaccggctg aaaaacctaa accggaaaaa tctgaaatgc agaaaaaaat
    29161 agacgaattt gccaaaacgc acatcgccgt caatatggac catctttccg ccaaggaaaa
    29221 agaaatggtc aaactattag tcaaagccgg acaaatcgcc gatggtattt tttggaagca
    29281 aacggatgcg gatgccgtta ggctccgcga tgaactgaaa gccaaaaatg atccggcctc
    29341 caaagaagcc tacgaatacc tgatgatcaa ttacggaccg tacgatttgg aaaccaatcg
    29401 tcgctttgtc gggcaagggc ccaccgaacg tccgttgacg ggaacgtatt atcccgccga
    29461 catgaccaaa gaagaatttg aagcgtatat caaggccaac cctaaagaag aaaaagaact
    29521 gaccggattg tataccgtag tcgtgcgaga tgggaaaaaa ctcaaagccg ttccttttca
    29581 tgaagtgtac aaagcggaag ttacggccat aagccaattg cttgcgcaag ccgctgcatt
    29641 gtgcgaaaat gtctccctca aaacatatct caccgagcgc gccaaagccg tattgacgga
    29701 cgactactac gcatccgatg tcgcgtggat gaaacttaaa gataacaaca tagacgtcgt
    29761 gatcggcccc atcgaaaact acgaagatcg tctgtacaac tacaaaacag cacatgaatg
    29821 cgccgtgatg gtcaaagatc ccgaaggcac caaagaactg caaatgttcc agtcgcactt
    29881 ggatgcgctt gaaaaaaacc tcccgtctaa aaaagaatac attcgctcct cggccggcaa
    29941 agccaatgtg cttgaggtgg tcaatattgt ttacttcggc ggcgactttc aaaaaggcgt
    30001 aaaaacgatt gctgcatctt tacccaacga tccgaaagtc catgatgaat acggcggcaa
    30061 aaaacagatg tataaaaatc tgatggaagc taagtttcaa aaaattctgc ttcctctcgg
    30121 cgaacgtctt atcgcaccgg attcacgtaa atatatagac atgcattcgt ttacgacatt
    30181 cgtaacgatg cacgaagtgt cgcacacgct cggacgcagt tttgtctatg gtaaagacga
    30241 tctgcctgtt cgtaaagctt tgaaagaatt gtattctgcc atcgaagagg ccaaagccga
    30301 cgttgtcggt atttacaata tggattattt tgccaaacaa ggcatttacg atgcggaaac
    30361 catgcgcaaa cattatacaa cctacgttgt cggtttgttc cgttcgatcc gatttggcgc
    30421 cgaagaagcg catggtaaag ccaacatcgc gcagttagca tttttgcaag aacaaggtgc
    30481 gttgggacgt gatgcgcaag gttattggca aattcatccc gaaaaatttc aagccggtct
    30541 gtcggcttta gctaaagtac tgcttgaaat cgaagccgaa ggaaactatg acggagctaa
    30601 gacgtttatc gaaaaatacg gtaaactgac cgatgcgatc aaagccgact tggccaagtt
    30661 tcacgatatt ccgcgcgatc tcaatacgac atacgatata atgaatcaac tttgatccgg
    30721 tcactttata aaccgatggc ggaatcatgc ccataacctg gatcaacggc aaatatgtgc
    30781 ccgaagatga aatcgctttg tcgcccaacg accgtggttt tttatacggc gacggggcgt
    30841 ttgaaacgat gcggtccgtg caatcgcgca tcatcgcatt gcatgcacat atacaccgtt
    30901 tgaaaacggc ctgtgaacga ttgcgaattc cgcttacaca aggacctgaa gatttccgaa
    30961 cgatcattca agaattgctc aaccgaaacg cgctgcaaga tgcttatata cggatcagta
    31021 taagccgcgg cccggttgag caatttggtt ttggattttc taaaaccatc aaaccgacct
    31081 gtctcatcgc cgtacgccct gcaccgacga ttcctgataa tatttaccaa aaaggcatat
    31141 ctgtcggatt tcagatcagt tcgctttttg gttcacacac cggcgagacg gctatcaaat
    31201 cattgagcgc ggtggaatac gtcctggcca aacaatcggc gcttgagaat aaatgctacg
    31261 atatgttatt gatggattcg atcgaacgcg tgtacgaggg cacctccagt aatgtattta
    31321 tcgtccgtca aggtgcgttg tgcacgccgc cggttcatag cggtatactt ccgggtatta
    31381 cgcgcgcacg ggtcatcact ttggctgaaa caaaacttca tctgcccgtg cgggaaatct
    31441 tgttttctaa acaagatgta ctgaccgcag aagaggtttt tttgacgaat accaatattg
    31501 atgtattgcc ggtaaccgtc gccggtgaac aaaccatagg caatggtgta ccgggaaaaa
    31561 tcacacagtc gttacttcaa ctgtaccgcc aaactctgat cgaaatcctt gaataacgaa
    31621 tccgccgtac atatcgaacc ttgggattgg ccggacgata tttggtcact gtatcgcacg
    31681 atcgccgcct ctccgcgatt tattttttta tacagctcgt tgctacaacc atcgtacggc
    31741 cgatattcgt accttgcatg cgacccttcc atcgttttaa aatgtacctc tcatcacaac
    31801 gagattacta cggctaccgg cgtgacatct atcgacggga accctttcga ttgcatgtac
    31861 gatctgatgc aacaatatgc cgcgccaaaa aatcacgcca acgttgattt ttgtacagac
    31921 ggctggtttg gatattgggg ttatgatatt cgcgatcatc tggaaaaaat cgcatcacgc
    31981 gcccgcaaga gtttgaatac gcccgatgcg ttatggatga aatttgacgc cgttatcatt
    32041 ttagatcacc aagagcatca ggcgtggatt accggtactc gtaccggcat tgagaaatta
    32101 cgcgagcgcc ttcaacagcc tgtagcctcg tatccagaaa aaccgcttca atttaaaatt
    32161 catccgaccg aaacccgtgc atcctacatt caaaaaatta actccctgca tgaatcaatc
    32221 gctgcgggcg atgtgtacga agtcaatcta tcgcagcgtt ttgatgtgac ggtagatggc
    32281 tacgatccga acgtgtacag tcgtattttt agatcgttgg tcgaaacaag tcccgcgcct
    32341 ttttcggcga ttattcaatg cgatgaaaca gctgtaatca gttcatcacc tgagctcttt
    32401 ttgcaaatgc atcaaaatat atgcaccagc aaaccgatca aaggcacacg gccacgcacg
    32461 ggtaattttg attccgacga aaaaaattat ttcgatttac aaaccgatga aaaagatcgc
    32521 gccgaaaatc tcatgatcgt tgatctcgtc cgcaatgatc ttgggcgcgt ttcacaggcg
    32581 ggctctgttg ttacgaaaga tctttttgct gtcgaaacgt acgccaccgt ttttcagatg
    32641 gtttctacga tacagagccg attgtccgaa catgctacga tgatggatgc cgtgaaagca
    32701 gcatttccac ccggttcgat gacgggcgcc cccaaaataa gcgccatgca acatatcgaa
    32761 tcgttagaac cttataagcg cagtgtttat gccggcgcta tcggctggat gcaagcttcg
    32821 ggggaaacaa tgttatcggt ggtaattcga acccttattt tcaacggagc ctccggttat
    32881 tttcaaaccg gcggggccat cgtgtgggat tctatacccg atgaggagta tcaggagtgt
    32941 tgggataaag ctcagggaat tctgcgcgcg cttgaaaaat tgggagtaat taacttaccg
    33001 gtatataaat aacctatacg tcatcctatc gcccatcgtc acatcatctg caatgcctag
    33061 ccgaaccggt tttttagatt cttcaaaatt tcatttctat tttttgtcag caaaagcggc
    33121 tatttgaaaa gcgcatccat cgagtaacct ttggcatcaa tgcttttttt gtatttttcg
    33181 tacattgaac gcaaaatttc tgttgatacg acatgggtga tgccgtaacg gtattctgtt
    33241 ttaaggcgag cgtacagttc acccttatat aaacttggcg caggaatttc ctgtgtaaaa
    33301 ccgggatccg gcatcagata atattcctgc gtagcggata tggatttcgc cacgcccaca
    33361 agttcaaaat taggcacccc gtctttgatg gcgagcacga tccctccgct gagtccttca
    33421 ttcatcggcg catcggtcag aaaggaaccg ttacgatcgc gattgggact gctcactgtg
    33481 cctttggtta ccattttaaa acccatcgga taaccgatca cgtacacaaa tgaaccccat
    33541 cgtaattttt tggattgccc gatcggatat ccgaaaacat gcacgggttc ctgtggaata
    33601 tgatcaaatg aacggccgat gatcgcgata tcatttttgc tatctatggc taaaacttcc
    33661 aactctccgc cttccggcaa atcgcgtata aaattgcttt gacgttgaag aaaagacacc
    33721 gactggacat gcccgctcgg ataataagaa tataccgtat ccgaaaattc gacaacatgt
    33781 gcacaagtaa gtaaagccac acgtttggta tcataataaa tcaccgttgc cgtaccggac
    33841 acagaggctt gcgtattaag ataacggtac gcgtattttt ctgccgcacc ggcgcgcacc
    33901 gccgtcggag ttatggcaaa tttttcatca aaatagtacg aattataaaa cgccgtggag
    33961 aacatgattt ttaccgaagc gctgatctct tccaactgat ccgaactgct tcggtatggg
    34021 aattcgctgt cgtattcatt atcaaataag gtcggataag ccgcttgacg aattcgcgaa
    34081 gaacacgaaa gcaagaggcc aaatgcgata atgaccatcg tacgcaataa tgttttcata
    34141 aaatttcccc gatttagttt aacgatgaac ctttctgcat ctctaccggc tttacacgag
    34201 ataagttagt ccggcagagg agttttcgca ctagtagtta aggctaacaa tgcaaaatac
    34261 attgaaagtg taatcaaatc tacgaccaat aaatacgata ttttattttg tgtgatctga
    34321 catatcgtcg catcggggta attcatggta ttcaatggaa tgcgcctaat aagactcttg
    34381 gaagcaattt atttttctgc gccatgggcg ataacgcatg tcggcatatg cggatcgtga
    34441 taaacgtttt ggaaatgcag tgaacgcttg catatgatcc aacgggtgat caattttgat
    34501 aaattcacgc gcttgcaatt ggatatattg cagcgtggga ttgatactgc gatgaccgag
    34561 tacacgctgg atcgtcatca gatccgtacc gttttcataa agatgtgtcg ccaagctgtg
    34621 acgcagggca tgcacattga gttgtttact gagccgactt cttttttgta atgtacggaa
    34681 aagcccttgc acataacgat cggcaagcgg ttcatcccct atttgccctg gaaaaagcca
    34741 ccgttgcttc ggttgcgtac gcagataatt ttcgatatga attaaaagcg tcgcattcat
    34801 aggtacataa cgcgctctgc cgcctttggc ataacgcacc aaaataagca tgcgctcccg
    34861 atcaatgtcg cgctgccgaa catgcaaaag ctcattcaat cgcatacccg tcgcataaat
    34921 catcataaaa attagccgat attttgcgtt aggttcatgc gtcaaaagcc gtttgacttc
    34981 actaatggac agaacaaccg gaaggcgttt ctcacgcgtc ggtcgcggca tcggaaaatg
    35041 attgataacc acgcccaagg tttgttcgta tagaaacttt atcgcgctat agacgatatt
    35101 aacgccggac caactatagt tttttgtttc acgcaaaaac ataagatatt gcccaatctc
    35161 gtcgtcgcgg atattttctg gcgctttttg aaaatgcatc aaaaaacaac gcactctgta
    35221 ggtgtacacg tcaaccgttc tatcactcat gtttctcaat ttcatttcca tgaccatacg
    35281 ttgaataata ttttccataa ctgtggcggg ttagatgatg aagtatatta aatgaaacta
    35341 tcataattta gatgcgtatt cacgatcaat cctcaatcat ctctctataa aggaaatgct
    35401 tatgcgcggc ttcacgcact tgtttttcgt acacctaaat ttagttatct tcggtatggc
    35461 tcttattaca ccgtgcctag ggcaaagccg atctcaagct gattccgtgg cggccgttag
    35521 aaaattacat ttaagtgcac tcaacaaaac ccttgaaggc cgtgaatcat taccggcgga
    35581 ttcggttttt aaaaacctgc agaccatcgg gggatttgaa gccggattaa tgcctgtcat
    35641 tatggaaaaa tggagtatcg ctttgggggt cggttgcgac tattgccatg atacaaataa
    35701 ctgggcttcg gatgctattc acgaaaaaaa gacggcgcgt cagatggccg gaccactcaa
    35761 tgaagcgata cgtaatgtac ttagcaaaat tgacggacta agtgaaagac ctgtggtcaa
    35821 ttgcgcaaca tgccatcgcg gcgaagtcaa accagctacg cgggtaaaat aaatcaattc
    35881 aagccattta cagccgaaat acctttcacg aaaggttttt gtcatactat aatattcaca
    35941 ttgtgtgatg cataggccga tttcttcaaa aatagtcccc tcttgaatac tctttttttt
    36001 tagcgtacaa tatgaccgaa gtcattcttt ttcccgtctc atgactctta attaagtgtg
    36061 aatttgcata cacccccggc tattaaggag attattccgt ggtcatagga attcccaaag
    36121 aaactgcagc aggcgaaaac cgcgttgccg tgacgcccga tactgctgcc aaactcatca
    36181 aaatggggtt tgaagtccgc gtcgaaaaag gcgctggcca aaatgctcat tatacggatg
    36241 aacaatacac caaacatggc gcggccattg ccgctacggc gacagacctg ctcggtcagt
    36301 cggatatcat tctcaaagtt caaaaaccca gcgatgcgga aatcaaacaa atcaaaaaag
    36361 gcgcattgct gatttcgttt gtgtatgggc taaattatcc gcaagtgccc aaagcgtgtg
    36421 ctgaagcggg cgtcaatctg atgtccatgg acttcattcc gcgcactaca ctggctcaaa
    36481 aaatggacgc cctgagttcg caatataaca tcgccggcta taaagccgtt ctgatgtgtg
    36541 ctgcggcttt cggcaaaatg ttccctctga tgatgacggc cgccggtacg atttcaccgg
    36601 ccaaagttgt gatcatgggc gccggcgttg ccggattgca ggctatagga acggctcgcc
    36661 gtctcggtgc tgtcgtcgaa gtgtccgacg tgcgcgctgc ggtcaaagaa gaagtgcaga
    36721 gtctcggcgg taaattcatc gaagtgcccg gcgctgaaaa tatgcaggat gccggcggat
    36781 atgccaaaga agcctcggca gaatttctgc aaaaacaaaa agaattactt ttcaaacaca
    36841 tcaccgaagc cgatatcgtc attaccaccg cgctggtacc cggtaaaaaa gcacctgtgc
    36901 tcgtcaccga agaaatgatc aaacacatgc gccacggttc catcgttttg gatatggcag
    36961 tcgaatttgg cggaaattgc gaagtcagcg aagtcggtaa aaccgtaaca aaacacggtg
    37021 taaccattat cggcgaatcc aatattcccg ccaccgtacc tacgcatgcc agcgaattgt
    37081 attccaaaaa cctgctcgca ttgcttcagt acatcgccaa agacggcaac ctacagctca
    37141 aactggaaga tgaaatcgtc aagtcggcgt tactggtaca taatggcgtg gtagtcaacg
    37201 aacgcatcaa acaagtttta taaccgatcc cggaggaaat atatatgggt gatatcgtta
    37261 tgctcatcac ggtttttgtg ctcgcctgtt tcgtgggcgt ggaattgatt tccaaagtac
    37321 ccccgacgct gcacacaccg ctgatgtccg gttccaatgc tatttcgggc attaccatcg
    37381 taggctcgct gatcagtgcc ggtatcggtg aaatgaccat cagcactatt atgggcgtga
    37441 ttgctgtggc ttttgccatg atcaatgtgg tcggcggtta tcttgtcact gaccgtatgc
    37501 tcaaaatgtt taaaagaaaa taattttctc agcgataaac caacaggttc actatggaac
    37561 atttccgcga aagtattgaa aatatcatgt acctcatcgg cgccgtgctt tttattttcg
    37621 gcatcaaaat gctcggctcg ccgaagacgg cacgtaaagg taatttttat tcagcggtcg
    37681 gtatgctgat cgccatcgtt gttacgttat ttgacaaaca cattcttacg tacgaatgga
    37741 ttatcgtagg cattttggcc ggctcggtca tcggtattat ttttgccgtc aaagtagaaa
    37801 tgacctccat gccgcagatg gtcggtttgc tcaacggatt tggcggcggt gcatcgacac
    37861 tggtagcctt ggcggaatat taccgcggta tggcgaccta tcaaactgcg ctccagtcca
    37921 atccatcttt gacactgctc gaacatatgc cctatcaaac caccgtttca atagtactcg
    37981 gcattcttgt cggcgcggtt acattcaacg gctcactcgt cgcgtatgcc aaacttcagg
    38041 gtttgatttc cggtaatagt atccgttatc ctatgcaaca tccgctcaac gcgctgatga
    38101 tcgttgttac gctggtcatc ggcgccttag gtgttatgga cttgaataac caatggtact
    38161 ttttaacggt cactatactc ggccttattc tcggtatcac gcttgtgatt cccatcggcg
    38221 gcgccgatat gccggtagtt atctcgttac tcaattccta ctccggtatt gccgcggcga
    38281 ttacgggatt tgtgatcagc aataatatgt tgatcatcac cggcgcattg gttggcgctt
    38341 cgggtatcat tcttacggac attatgtgca aaggtatgaa tcgttcgctg atgaatgtgc
    38401 tcctcggcgg ctggggtacg gcgccgactg tcgaagcggc taaagataaa aaacaagtgg
    38461 tcgtcaaatc ggtggatgtc gaagaagcgg cgatgttgtt tgatgccgca cgtcttgtga
    38521 tcgtcgttcc gggttacggt atggccgtcg cgcaagcgca gcatgctgtt cgcgaactga
    38581 tgaatgtact cgagaaaaaa ggatgcgccg ttaaattcgc tattcacccg gtcgccggtc
    38641 gtatgcccgg tcacatgaat gtgcttctag cggaatccaa cgtaccttac gaacacatgt
    38701 attcgctcga ggaaatcaat gatgatttcc gcaacgccga tattgctctc atcatcggtg
    38761 ccaatgacgt cgtcaatccg gccgcacgcc atgaccagtc cagcccgatt tatggtatgc
    38821 ctattcttaa tgcggattac gctaaaaccg tgattatcaa caaacgctcc atggcggccg
    38881 gttatgccgg tattgataac gaactcttcg gattccccaa tgcattgatg tattttggcg
    38941 acgccaaaga tgccgtttcc aaactcacca acgagctgaa gagtatgaat aattagaacc
    39001 ccccttcgtt tgatagacgg ttacacacaa tcgccatgat cacaaatgtt catggcgatt
    39061 gtccttttta tcctttatct ttttttatga agcgttttac tatcgtacgt tatcaacacg
    39121 tagtgtatta tacaggcttg tatttggtca ttttcttttg gttgggatca atgccatccg
    39181 tcgttgcgca gcgttcattt ataatgcaaa atgaaaccgg tgtaattacg caagaccgtc
    39241 cttccggcaa ttcgatcggt gttattacca caaccggtga tacagttttt atcgccgcag
    39301 gaagcaacct caatatcacc acaaatgcgg gtgcagcatg gaacatcatc ggacgctctg
    39361 acggattata caagggttcg cctgtcgcca tcacgcatca taaatccgtt ttgtatgtaa
    39421 cgacggctta cgattcgtcc ctcgccgttg agccaaccta cggcgtcagc ggcggtattt
    39481 ccatttccaa agatttcggc agcacttggt cgcatattgc acaacccacc gacaccggca
    39541 ccattcgagg acccgtcgta cgaacggatg cacgtacatc gcaaccggtc gttcttaatt
    39601 tagtacgcga tgtgatgcga cgacaaaccg gtttccttac cgattcgatc tatgtcgttg
    39661 cgatcaaatc ggcaatagat aatat
//
LOCUS       DAOQGF010000017        39408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_437_length_39408_cov_6.301324, whole genome shotgun sequence.
ACCESSION   DAOQGF010000017 DAOQGF010000000
VERSION     DAOQGF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 39408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_39408_cov_6.301324"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PK937_04245"
     CDS             <1..767
                     /locus_tag="PK937_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH31813.1"
                     /translation="TEPDHELTAVIADDEVLARKRIRHLLAKRSDIRVVAECVHGYDV
                     CDKVLEYKPDLLFLDIQMPEMDGLEVLRTLGPVAVPGVVFVTAFDQHAIRAFELHALD
                     YLLKPFDDARFYEALDRAIQMIGKQQLPDNLKRLLQNLPRPEAFMPRIPVKDGIRIHF
                     VETANILWIEATGKNTMMHTTDGSIKTAKNIIEVARDLDPTQFYRIHRSYIVNIRAIR
                     EMQSWHKGEYVVILSDATRLVTGRAFRGNVDLLMRR"
     gene            984..2129
                     /locus_tag="PK937_04250"
     CDS             984..2129
                     /locus_tag="PK937_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31814.1"
                     /translation="MKTFIWLWLTITCIGSTSCRDRSAGVQEPNALSQDDKDVLFQIH
                     NGDLAGAESKINAMLSNNADNLRGHFLKTSLYFHARYFASQGASRDSITTLLLKSCEE
                     AITQAEKQSESIEQQFYLGSFYSFKSRALLAKREWTSAFFAARTGKNLLESVIESAPG
                     YADAYLPLGVLYYFASTQQAWWQGALAWVADLDVPKTQALTYIQKTYDEGLLNKNEAH
                     FVLNNINLFFENKLDIAESLLQNYNAQFPNNRFFLAPMQGLKLTRIAEEKGGEFLLTH
                     LDSLVTAHPLNGMQTLNRVGYRYLGQKKFPDALAVFHANVKLFPGSANCHDSLAECYY
                     TMGQMQESKKYYSLALTKLSGDQTINEEFKKTLKEGIEVKLIDLASY"
     gene            complement(2186..2259)
                     /locus_tag="PK937_04255"
     tRNA            complement(2186..2259)
                     /locus_tag="PK937_04255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2223..2225),aa:Val,seq:gac)
     gene            complement(2272..2357)
                     /locus_tag="PK937_04260"
     tRNA            complement(2272..2357)
                     /locus_tag="PK937_04260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2321..2323),aa:Leu,seq:taa)
     gene            complement(2371..2444)
                     /locus_tag="PK937_04265"
     tRNA            complement(2371..2444)
                     /locus_tag="PK937_04265"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2410..2412),aa:Cys,seq:gca)
     gene            complement(2567..2639)
                     /locus_tag="PK937_04270"
     tRNA            complement(2567..2639)
                     /locus_tag="PK937_04270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2604..2606),aa:Gly,seq:gcc)
     gene            2934..3809
                     /locus_tag="PK937_04275"
     CDS             2934..3809
                     /locus_tag="PK937_04275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH31815.1"
                     /translation="MNSKKALIIVFVWVFGLSSCALFIDPYEAQYRQLLHERDSLQLS
                     IARTRHDLKDLEAELWKTLYDTEYLRNALVLAQADSVKNFYYNYVFRYRRPIQAIAEI
                     DPASPFLFPLKTEKDQNRIKFRIMWGRLFYFDFNHLGIDIRALEGDTVRAIYDGVIQN
                     YGGAEGYGELVAVVEHEYRGLWNKDVVPQRFMSIYGHIRNRCYDGEPELKWKAGDRIR
                     KGDIIGFINDDSRNGYGMEHLHLGIRRQSAMDAQAVDGGYWMRGYDTPKNSKFQFFMN
                     PIDLFGRYVQFVLPE"
     gene            3906..4793
                     /locus_tag="PK937_04280"
     CDS             3906..4793
                     /locus_tag="PK937_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNH31816.1"
                     /translation="MRLITRTDLDGLTCAVFITIMEKIDEVVFYEPKEMQDGKVPVTD
                     RDIIANLPYHPNCALWFDHHQSQLIHQNAEYRGKFAVAPSCARVIYDFYEQPDELKPF
                     KELLEETDRVDSAQLTMEDVMNPKGWVLLSYTLDPRSGLDAFTDYFQRMIGWIMVHTV
                     DELLQLPDVKSKVDKYLADQDKFKEALTTYSREDGNVIITDQRSVDKFPSGNRFLIYT
                     LFPKANVSVRLFKGKEPGVTVCAVGHSIFNHSCKTIVGELMHEYGGGGHKGAGTCQFP
                     EAEAEAKVAEIIERLKKNG"
     gene            complement(4908..5960)
                     /locus_tag="PK937_04285"
     CDS             complement(4908..5960)
                     /locus_tag="PK937_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNH31817.1"
                     /translation="MRFSLFCFMITIHVLPTWAQNNIPEWSWSGFVDAYYGYDFSKPV
                     SGDRPGFLYNHTRHNEFNVNLALLRVQAKYDEAYARVGIMAGTYAQSNLSAEPATYQH
                     LYEAYTGIQINAKTSLEVGLFGSHIGWESAVSKDNWTLSRSLAAENSPYYLSGVKATY
                     SLSEKWTFAAVVCNGWQRIYRSSKNVTPAMGTQIVYKPSDRLTINYSTFVGNDKADST
                     RQWRYFDNAYAIWQVNPKLGITTGVDFGAEQKSKGSSKYNTWLTWAIITRYMLNDMWS
                     VAVRAEQFLDKNQVIVSTGTVHGFQTLGLSANLDYALTKQSVLRIEGRQLQSRDAIFS
                     GETSNLSVLSSLAISF"
     gene            complement(5989..6531)
                     /locus_tag="PK937_04290"
     CDS             complement(5989..6531)
                     /locus_tag="PK937_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH31818.1"
                     /translation="MKALRLLLIVLGILIALPLLVALFVKKQYAVERSITINQPNQVV
                     FDYVKFLKNQDHYSKWATMDANMKKTYRGEDGQVGFVSAWESQNPDVGKGEQEIVKIA
                     DGERIDFELRFFEPWQSKEPAYMTTSAATDSTTLVKWGFSGHMDYPMNLFLLFMDMEK
                     MIGDDLATGLANLKTVMEKK"
     gene            complement(6606..6833)
                     /locus_tag="PK937_04295"
     CDS             complement(6606..6833)
                     /locus_tag="PK937_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNH31819.1"
                     /translation="MEPFHDFGKTLMILGGVLIAVGMFLTFSDKIPLIGRLPGDIFIQ
                     RENFSFYFPITSGILLSVILSVLFWLFRSKY"
     gene            complement(6845..7672)
                     /locus_tag="PK937_04300"
     CDS             complement(6845..7672)
                     /locus_tag="PK937_04300"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNH31820.1"
                     /translation="MSPLTAQIAEAVNYIQSKAKTQPSVGIILGTGLGGLVQEIRKEI
                     EIPYQDIPHFPLSTVESHAGKLIFGTLGGKHVVAMQGRFHFYEGYTMQQITFPVRVMK
                     FLGIKALLVSNACGGMNPQYQPGDIMIMDDHINLLGDNPLRGKNEDTLGPRFPDMSEP
                     YSRTLIALAENIALEEKIKVQKGIYVAVMGPNLETRAEYRFLRYIGADVVGMSTVPEV
                     IVANHMGIPCLGFSIITDECFPDSLQPVDLPKIIKTAGEAEPKMTLIMKRVVEKMNL"
     gene            complement(7669..8553)
                     /locus_tag="PK937_04305"
     CDS             complement(7669..8553)
                     /locus_tag="PK937_04305"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNH31821.1"
                     /translation="MKLNPGDIQHQEFKRVMRGFDPDEVTAFLEVVAENYKEILDETK
                     LLQQKLKAIQDETQYLRLRIQSSEKSLNDYKQEMVKVEKLMDARVDADLILEQARAEA
                     ERIRTQAQKENESLAQEVNFLQLQKTKVAAHLREYLKSQIALLEIVGDQKAEAAAKTI
                     PVPEKVEPRVEKPVEIKETELPPPIIPVATEEELKAGLEEGIGSFVQDLKLDDLPPEL
                     AAAIRESADDTAITDPERHEKKKKVIEELEKLTHNATGIFKKSDFEKMLGDSADKKSH
                     ELLDQIHKELEKKRSEKS"
     gene            complement(8550..9323)
                     /locus_tag="PK937_04310"
     CDS             complement(8550..9323)
                     /locus_tag="PK937_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNH31822.1"
                     /translation="MDIALQTTIRANIETIRKKIEASARKVGRDPKEIHLIAVTKTYG
                     IDYVREAVAAGISLIGENKVQEAKEKYTELEQSGNVPDFQLHLIGHLQTNKARQAVEL
                     FDMIHSVDSFRLAKEIDRRAYTVDKTMPILIQVNTSREATKYGVEPDNTLELIKQVSE
                     LKNIRIQGLMTIGALTAVQANDPEKIRSYFRRLRELRDFIREQNIPNVEMNALSMGMS
                     SDFEIAIEEGANYIRIGTALFGKRKRAAKSAAPKSEETL"
     gene            complement(9344..10396)
                     /locus_tag="PK937_04315"
     CDS             complement(9344..10396)
                     /locus_tag="PK937_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31823.1"
                     /translation="MNVRSHIASIQIGILVDFQIYTAYLCFCIQTMNRIVFSMSTAEH
                     ISPAASAMTETPDSVFRSQRILALDPQVFNSEEIQVIHLSLLQPDAFRELLDRFNYHQ
                     LNNLLDALIKDGILYYQEKHGVTTTERTVRTRCENLLDYKERIGCVEPVESCLHEGCI
                     HLKPACAVRKVKEDVLNVARLFEARKNRSTVPHVAIELFFKKLIEDYNCVGLILADKW
                     GTPKVFTTDGSGVLSVISEGSRRLTDAITDREFRFTKTGDSYQVFNQKFHIDAKLAER
                     LHAVEKNPDLTKFIKRITFSSIGIEVAHEKIILSVLYMGDNLLHQFLGKTISGIERIK
                     LETEPELRIRAGFGRK"
     gene            10609..11301
                     /locus_tag="PK937_04320"
     CDS             10609..11301
                     /locus_tag="PK937_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31824.1"
                     /translation="MNKRWMIFVVWCCAGVLSAQQYQVRISPDTILCSDQEAIIRVSP
                     GRSGVELTAVARKGGGSIGDFRYANSDQGYVNQAIFSSPFSGEIEIEVMDNKNNKVGM
                     VTVTVVPPTMEFMEDESIAESNAAEKRIPLKIKVLDQNGSFIKTAKVTCKVSEVEGTG
                     KNKKIKPSDAKVGAFVLLDGYYEGQLTNLKNATYRLEFFDEAHLLPFDSEENPDAQHP
                     SLLIDSWPISFN"
     gene            11455..13194
                     /locus_tag="PK937_04325"
     CDS             11455..13194
                     /locus_tag="PK937_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH31825.1"
                     /translation="MIKPFSLRLDEPRGWGFVLLLLCGCATIGAPGGGPEDKEPPTVI
                     TTFPAKDSTGVSRQTEVTVTFSEAMNKASVEKALFIAPFPARDPDLSWDDATLTIRFK
                     DALEENRTCVITIGTDAMDAHQNKFKSAWSFAFSTGDKIDAGSIAGTVVSESKTKPSV
                     WAYRLNGHTSSRDSMIYTKRADYITQVDAQGKYRFSYLADGAYRIYAVSDQGEDFRYH
                     PMQDMIGLAPRDVILADTSKEALFVDIAVFREDTTTMAVQLAQMTGPNEVLIRINNVL
                     AESRYDGTKIGSEHIASHFSIRDSLSGTDVAIRDVYFVTTGATEEIRLLCDSMSSQRV
                     YVLDVRGIYGLSGDSIAFQQINFRAEGGFETLRAKIEWTFPYGTPGLIAVNDFIGWKS
                     DRGLDRPTWEKAFVLRDTLGNSVNGTFFWMHSASGKFIPARNLRGRMPYEVHVDGKIL
                     QDWSGRPAFDTTMVWRLVAMPEDTVGTVSGMIADESSDDRGMYHITARNLNRSGRDYT
                     LIVPSPGGFTLDGMLPGKYNFLAFRDRDSNGVFSFGQTAPIIRPERFTSYSDTVTVRK
                     NWETSNIQIRFRK"
     gene            13198..13608
                     /locus_tag="PK937_04330"
     CDS             13198..13608
                     /locus_tag="PK937_04330"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNH31826.1"
                     /translation="MKSIYYLHQIKTGKNFRVEENQEVTIGRAFDNTLMIDDNSVSRY
                     HAQIKWKKNLMYITDLQSTNGTSVNGEKVEHGYFTELNFSDLVKIGNIELKILDEESV
                     IGKNFTPDQAPAKTIVINQAMLKKAVDPDDFTHN"
     gene            13625..15241
                     /locus_tag="PK937_04335"
     CDS             13625..15241
                     /locus_tag="PK937_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31827.1"
                     /translation="MRIKLSLWVVGITLSVMIGCSSRTAVQPDIKNNTTASASTDDHG
                     HKTETLAEEKKGPSEPVITVVSVPKTVLSAEEKMELRAVGDSLYEKGDFEEALPVWVK
                     ILEHADDKPTIAAAHYVLGTLFYQRQEYFKAELEFKRAVESDSTLIDVYRELGFVYFV
                     KGDYDKARASFETMLNYLPGDSEAVHWVDYTIGTQAYEEGLKLFTDESYDKAIEKFQS
                     AIAYLEEDTSANYKIHYFIGKSYLEKLDYDNALKHLSKAVSLRPEMHEGYTEIGAIFF
                     SRSDYPKAIGFFQRALTYQPSSSKALNNLGYVYFTLGNQALAGGDKNRSDEFYKKAVT
                     YFEQALIADPAMAGARYNLDHVRKILSGARKATAYTLLQAALKTENHAERIRTYYKAI
                     EQDSTYDDAYVNLGVAMFYHGQADSGMIMTEKAIAINAYNAQAHNNLGYMLGTAHRWE
                     ESLKHIFLAIQIKRDYTDAYVNLGYVYMWKEDFESSRKVWLQLLKNNPDNTMARKGLA
                     EMERREKMVRSGETTTHIEINEDRAGGGSN"
     gene            15336..16346
                     /locus_tag="PK937_04340"
     CDS             15336..16346
                     /locus_tag="PK937_04340"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNH31828.1"
                     /translation="MSESKTIRALAVHAPGALLSSFEYGRTSLGADEIEVKVEYCALC
                     HSDLHLMDGDWACAFPFVPGHEIIGSIIQTGVSVRDLKPGQRVGIGWQCGSCGICEVC
                     ITGRAHLCEAGKKRTCVNQYGGFAEYVRAHQHFVYAIPETIPSIHAAPLMCAGLTVFS
                     ALQTWVTPKTRRIGIIGMGGLGHLAVQFAARMGCEVTAFDLSEDRLTLAKELGAHQAA
                     LTDSAPKGTFDLMIAATAASLDWNHWLRHLTWGGVLCLLGYPAEEIRISPDILLDGQK
                     IVTGSVVGSPETMRTMLDFAARHHVRPMVEELPMKQAHEAVARLRANKVRYRMVLKAD
                     WE"
     gene            16467..17543
                     /locus_tag="PK937_04345"
     CDS             16467..17543
                     /locus_tag="PK937_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNH31829.1"
                     /translation="MIYPVIMAGGSGTRFWPRSRAHRPKQLLNIFGEHTMIQHTVNRI
                     ASIAKPSEILVVTNKAQASEVHRQLPQLPSSNILVEPVGRNTAPCIGLAALHVMEKDP
                     NGVMVVLAADHLITPDDVFCKTVEAAAEVALRTEACVTIGIVPTRPETGYGYIQFIEN
                     ESLEAHGEKAYRVKTFAEKPNLETAKSFIATGDFLWNSGMFVWKASTILRLIEMHLPE
                     LYEGLVEIRPTIGTADYTATVDQVYRRIKGISIDYGVMEHTKEVYVLKGHFRWNDVGS
                     WEEVYQLSNKDENGNAVIGQAVCIEANNSLLFSPGKMIALVGVSDLIVVETEDALMIC
                     PRDRAQDVKKIVETLQAQKKNELL"
     gene            17551..17844
                     /locus_tag="PK937_04350"
     CDS             17551..17844
                     /locus_tag="PK937_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31830.1"
                     /translation="MKKEKILDTLEDIAAKLGLKVRHEKGDFAGGLCVKDDTQWILLN
                     KRHMIDRRINILARELAPFDLTGIEVPKPVMTIIESERKRATQDELELTDSEE"
     gene            18006..19874
                     /gene="uvrC"
                     /locus_tag="PK937_04355"
     CDS             18006..19874
                     /gene="uvrC"
                     /locus_tag="PK937_04355"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNH31831.1"
                     /translation="MNDTLEHKLEHLPKTPGCYLFKNGEGKIIYIGKAKILRNRVRQY
                     FQEGKRDDTKVAVMVSKVVDLEVIQTDSEIEALILESNLVKEHKPRYNIELKDDKSFP
                     YIKVTDEMFPRIYVTREKERGGGKFFGPYTDVKNLRHTLKTLHRIFPIRSCRHYFTPE
                     LVESKKIKLCLDYHIKKCDGPCQGLVTKEDYRAVVDQMTRFLNGKTGELIRQLKVAMQ
                     SYADAMKFEEAARLRDRIASIENYTAAQKVMFEDMADRDIAAVAIEADDACGVIFKIR
                     DGKLIGRQHYYFSHVTDKAPEEVLESLLQGYYMQADFVPRHIYLPQALSEETALTTWL
                     MQKSGERVELVVPKIGEKHKLVTMCERNAELLLGELKLQRLKAKEQFVPRVLESLKRD
                     LSLQRIPRRIECFDNSNIQGTDPVASMVVFVDGKPKKSDYRKFKIKTVEGPDDFASMN
                     EIITRRYLRVLEEGLDFPDLIVVDGGKGQLSAAVKALEALGIVVSRAGSEGQAIVGLA
                     KRLEEIFIPDVHDAIMIPKTSSAIKLLQHVRDEAHRFAITFHRSLRDKRTLVSEIDQI
                     EGVGEKRRTLLLQHFGSVQALSEAGLEEIMLVEGIPGDVARQIYAYFESRDDEESH"
     gene            19935..21776
                     /locus_tag="PK937_04360"
     CDS             19935..21776
                     /locus_tag="PK937_04360"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNH31832.1"
                     /translation="MSEFLETLAKRVLVCDGAMGTVLYEKGISFDHCFDEINLSHPEM
                     VRAIHEAYIDAGADIIETNTFGGNRFRLMHHGLEEKIQAVNAAGVRIAVTTAKYKTEA
                     LGRRIFVAGSIGPLGKPLEPIGKITHAEARAYFAEQAKILADNGADLLMIETIGDLEE
                     AREAVTGARSVTGLPIVAQMTFNDEAKTLMGNKPSEVARVLKEAGANIVGANCSVGPQ
                     ALLEVLEKINIVPDIGFSVQPNAGLPRLVGGRYIYLASPDYFSDYAKLYMQAGANIIG
                     GCCGTTPEHIRQIKKAIGDQPPKKIEKKTAVIETEESSKAEQPPSHLRSSLIDKFRDK
                     KFVISVELDPPRGLNYDKVIEGAVMCRRHNVDAINIADNPLAKAGMTPLAMAGLIKQS
                     VQIETILHFSCRDRNLIAMQSELMSAHVMHIRTILGITGDPPLVGDYPNATGVFDVDS
                     IGLINLIRNLNNGIDLAGKSIGSRTNFFKSCAVNPTAVDMAREYDRYDQKIASGADFA
                     MTQVLYDLKPLEDFAKKYKGKIPVLLGIMPLKNAKHANFMHHEIPDITIPENIRERMT
                     KAGDRGQEEGVLIAKEFLREAKSMVDGVYIMPPFNKFEMAFDVLEVL"
     gene            21789..22733
                     /locus_tag="PK937_04365"
     CDS             21789..22733
                     /locus_tag="PK937_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HNH31833.1"
                     /translation="MKPTEIKTEIGRSEATHRQSSRGALWRNMLAAMRPLHWVKNIFV
                     LVPLLFSASEWTSVQIVYISAAFGLFCLAASAVYLFNDVVDREEDARHPSKQLRPITR
                     GLVSVHDALFMATILAFASIGGAYALAPALAYVIAGYVALNIGYTWFLKRWVIVDAVV
                     IALGFVIRIWAGAAAVNIPTGTWMLVTTFFLSALIAFSKRRYEIVYDGTEAFHTRGYT
                     PYLLDMLILLSAASVIGSYVFYILARGTWQHEYIILSTVLVVFYGTMRYIYVIHRNEE
                     RLDHTRLILSDRPLMAAVILWTALMMVAIHRVALPIGL"
     gene            22733..24181
                     /locus_tag="PK937_04370"
     CDS             22733..24181
                     /locus_tag="PK937_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH31834.1"
                     /translation="MGSPIRHIWFFNPPGALFQRGEDRCQANVEASSAVSLRAPNDLA
                     YMASVMRAAGIRCAITDFPAEGGTWDDFEKQFSALNPDLVVMSITNATLRDDMEAFRR
                     IKKNNPSVITVAKGAWFWAGDLRGLSEPVFEAMDYALVGEAETEIKGLVEGLHAHTAP
                     GDIPNIIFRLPDGTWKRTRIEAFETDLDAIPFPARDLLKNDLYVRPDTGEAQATIQTA
                     RGCPSQCVFCLTPAISGARVRSRSPQNIVDELEECVTRYGIRNFFFKADTFSINKKFV
                     IALCQEILRRNLDIAWVANSRVDTVDEERLAWMKKAGCWLVAFGFESGNDDVLKKMKK
                     EATVEDARRAAALVRQGGLKMYGFFMIGLPWDNDQTIRDTIALARELACDFYEIHLAT
                     PFEGTELYTIAQSMGLLTTAPLGHDYFADPASGSLFLSRDQLIEYRKSGLRSLYLSPS
                     YIYKTARSVNSPKVLLNYARYGVRLFRNLLKA"
     gene            24185..26200
                     /gene="ligA"
                     /locus_tag="PK937_04375"
     CDS             24185..26200
                     /gene="ligA"
                     /locus_tag="PK937_04375"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNH31835.1"
                     /translation="MHDHIQKKVHKLRDEINRHDYLYYVLGQPAITDEAYDKLMRELS
                     EIETRHPELVTPDSPTQRVGSDLTKHFPTVRHHQPMMSLGNTYSREELSDFDRRVREG
                     LEGASFEYVCELKFDGVAMSLIYEYGHFVRGVTRGDGEKGEDVSINLKTIRSIPLRLD
                     AANAHLEHIEVRGEVLMLREDFIKVNARRTEAGEPPFANPRNLSAGTLKLQDPRLVAA
                     RPLKFFSYYLRELGAEKKLNSHSESLTALESLHFPVMKTYRVCKTLDEVFAFCEEWEA
                     KRDTLPFEIDGAVIKVNAFRQQETLGATAKSPRWAIAYKFKAQKVETRVRDIVLQVGR
                     TGTVSPVAELEPVFLAGSTISRVTLHNEDFLQEKDIRIGDTVRIEKGGDVIPKITDVV
                     FDKRPAEAHVFRFPKACPVCGEPVVKTEGEAAWRCENITCDAQIKKRIEHFCSRDAMD
                     IENCGEAVVAQLVDAGCIHDFADLYYLQKEALLALERWGEKSATNLISAIEHSKHNSL
                     ERLIYGIGIRFVGEESAKYLAKHFKSLDALRQADGETLTAIEGIGERTALSIKQFFDS
                     AQNRKVLDKLTQAGVQTMYISSAVELPPIFSGKTFVLTGTLPSLGRNDAKKMIEDRGG
                     KISGSVSKKTDYVLAGAEAGSKLDKAKELNVPVIDETEFLKMIGDAP"
     gene            26197..28002
                     /locus_tag="PK937_04380"
     CDS             26197..28002
                     /locus_tag="PK937_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH31836.1"
                     /translation="MKRTLTLKWLSVLLMGLVTAPLLLSVHDARSYKISPRLKAVLAD
                     SEPETNVHVWIYFTDKGQTALPKFNSESLGISPEAVMRRQKMGTRHATDEADWPVETS
                     YIELLRQAGVQIRHTSRWLNAVSAEVPAGRILEITEWSFIQRIDRIQRMKRTKRIAET
                     ENNPMPLPLSKKSSLDYGASLAQLSLLKVPEVHQYGFAGQGVTIAVFDAGFDNLSHEV
                     FSKMIREGRLLADSDFVNGDDDVSDEGDLGEGSHGTQTLSTMGGYKPGQLIGPAYKAS
                     FLLAKTENTESETPAEEDNWIAAIEWAERRGADVISSSLGYIQFDATCCNHYDSTDYN
                     WQWMTGDSTRITQAANMAVARGVIVINSAGNEGDATVVIKNTLGAPSDGDSVIAVGAV
                     NTSGVRTSFSSVGPTTHGAIKPNVMATGSNVRAAGSTTNTSYVGVSGTSFSCPLTAGI
                     CAMLLSAYPDLTPAQVRYALQATADRADNPDNHYGYGVVNAWDAINYFGQIDSGGILP
                     DKFKLHQNSPNPFNPQTTIRYDVLRDGYVAIIVYNMLGQRVRTLYDGFRSQRRNYEVQ
                     WDGRDESGHRVASGVYLYRLTADGFSQTKRMLLVK"
     gene            28095..29858
                     /locus_tag="PK937_04385"
     CDS             28095..29858
                     /locus_tag="PK937_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNH31837.1"
                     /translation="MNIAVAMLVSLGALWLAYRFYGSRVAQWLGVDVSRPTPAQRVND
                     GVDFVPTKPVVLLGHHFASIAAAGPIVGPTMALLYGYMPTWLWIILGVTFIGAVHDFS
                     ALFVSVREDGRSIAEIAKRTLGKTGFLFFVAFAILLCILVCAAFLQLTAVSLTSLYPL
                     KEFGLPEDQNILKTVTDNGVLKGVIGGIASTSVIVMTLVAPLIGYLLYKKESGILTVS
                     LLAFIFCLVSVWVGFVYPVTLDPKIWMMVICAYCFIAGWIPVWLVLQPRDFTNVQLLY
                     FGIVVMVAGIIGSGLAGVVIDAPAFNFSAESFQALGYMWPFLFVTIACGSVSGAHALI
                     ATGTTSKQIGSEKHAPMIGYGAMLLEALLGICVTLIILGGLGYTDYHDIVWPHDEFGN
                     PKAGNAPLAFAVGVGHTLDKGLGISVVYGTVFGIILLEGFLVTTIDTIVRLTRYLFEE
                     LWLTLMENPHPIFRNKFFNTLVPVIGMIALGFTNAYTAIWPIFGSANQLLAALTLTAI
                     TAWLIQKSRPFWFTAIPAIFMCLTTITSLSLLFKRYLDARNWALTIADAILLLLTVGV
                     VIMTFRYFYNLRKELSSKKSV"
     gene            29867..30580
                     /locus_tag="PK937_04390"
     CDS             29867..30580
                     /locus_tag="PK937_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31838.1"
                     /translation="MKNYLLLYIILVFAGVVSAQTGLARRQADQLYDRGDNFHAMEAY
                     KNLLNDNPNDPELNYNAGNALYRLEQYDEAVKFYQRALENTSDADLKNKIAYNMANIQ
                     YKQKKLKESIEGYKNVLRKNPDDADARKNLEYALKQMQMQQPPPQKNQDKNQSKQDKK
                     EQDQKNQDDQNQPQDENKEEQQNDEQQNKKKSDPNKSGLTKKEAEKLLDALKNQEKDY
                     QKKKIKDKAKSDSETEKDW"
     gene            30612..32804
                     /locus_tag="PK937_04395"
     CDS             30612..32804
                     /locus_tag="PK937_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31839.1"
                     /translation="MFYTSIQLTFAWSVFFLIVIVGLSVGLSAWLYRETIPPVSQVRR
                     RILISLRSAAFAIAFFILFEPILDLQYEEKEKPVVAVLIDASESMSIRDAQGDRQQQR
                     RGVLDHRMWADHADAWDIRYFTFSDRLYPSDHKPSDTLALTGHATDMRTALEQTQSLF
                     IGKHLAAYVMITDGQYNIGGDPGGFAQVSDVPVFTLGIGDPAESRDVAIVQISHNETA
                     YLGATIPVTVTLSSYGYQGKRIDVEILHNGKRVQNKIVVLPEDGALTQVTMDVIPDEI
                     GDQKYTVRASPLLGELSDRNNQRAFVIRVLKNKTSVTLISGAPGPDHRFIYSVLIENP
                     NLDVRAYVEQKNGTLTELEQPPGRTSPTEADVYIFNDYPTNTSDPSTLQRFLQDISDN
                     NRSAAIFFGNHIEVGRLQSIQSRLPIKAKPDASLQESSVFIGMSGSASQSTIVAVSDD
                     PGETMRQWADLPPVWISRYAVTASEESDVLARVDMSRTTNTLRLRNDIPLLITKRLRQ
                     QKTLAFTGYGFWKADFIIKGLNKSNAVYPAFIQQSVQWLAAKDDTKPVIVRAGKSVYR
                     SGEAVMLTTQVYDEQFRPISDANVNVTLQNGSASHMHAMQPLGNGRYEITLDGLMPGD
                     YPITSSAERFGALIGTDKTAVTVEAFSLEMSSTAMNARTLQTIASASGGRFFASSGID
                     SLAGVMNFQPLIFEREKNIPVWNATALLILIILLLSAEWWIRKRSDML"
     gene            complement(32799..34907)
                     /locus_tag="PK937_04400"
     CDS             complement(32799..34907)
                     /locus_tag="PK937_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNH31840.1"
                     /translation="MHALDAIFKPRNVAVIGASRKSKTIGHEIIHNLIECGFTGAIYP
                     VNPNAPAIHSIKAYPTVKDIPDEVDLAIITVPKQLVLGVVDECAAKGIKGLIVITAGF
                     KEVGAEGAALEDQLMAKIKKNDMRMVGPNCMGVINTDEHVNLNATFSPTQSRSGNIAF
                     ISQSGALGVAILENAESLELGISNFASIGNKPDISGNDLLEYWRDDSRTKLILMYLES
                     FGNPRRFIEVARSITRFKPILAVKSGRTMAGARAASSHTGALSGSDTIVDAMFRQSGV
                     MRVSSIEQLFDVAMAFDKQPLPHGDHVAILSNAGGPAIMATDALVSNGLKLAEFTQQT
                     KTALRQILSPEASVNNPVDTTAGGDAQIYGKALDIICKDPNVDSVLAIFVPPITVFLH
                     DVAEQIVAINKRFPDKTVLSCCMSQEKIHISYMRTHDIPTYTFPESAALALAAMNEHR
                     KWRLKQEGTLRKFEVDKAAVEKIIRDCYAAGRTYLSQDEVFGILRAYGLRTASSTIVK
                     TGAEAVAFAAKLNKPVVLKVHSSQIVHKTDVGGVIIDLRTEADVQRGYQTIIKNLTAH
                     HLQDKIEGMLVQEMVKGGKEIVLGMSRDPQFGPVLMCGLGGIYVEVMKDVAFKIAPLT
                     DIDAREMIESLRSYPLLQGIRGEKPADIATVVEYLQRLSQLVTDVEAITEMDVNPLMV
                     FEKSEDCKIVDARIKLKEPS"
     gene            35091..36272
                     /locus_tag="PK937_04405"
     CDS             35091..36272
                     /locus_tag="PK937_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH31841.1"
                     /translation="MPKYPHTAERVKELTGSVYEKYRHLIKQQTSVAKLHIGDTALWP
                     PYAMPLADYFTSAHRDMTKYCNTYGVDELRKSIAEKVSEDNGITSTRDHVLVTAGASN
                     ALSAAMMTLINPGDEVIVFAPCWPFFTGMVRAAGGKVVEVPLYMDLYDQPEMDIFGKL
                     KNATTSKTVAVYVNSPNNPSGKMLDARQWQTVVDFVKQHQLWLISDEAYDGLAFDGRK
                     TISPATLGIREQTISIFTFSKIFMFAGLRLGYAIAEPELIHALNKVMVHQLYSPSPVG
                     QYMMVEPLRRRHEWMHGINAQYQQLRDAMVEALAMPVAKPEGTYFVFLDAAPYLQGRA
                     FDQLVEQMIMGGVLVAPGGDFGKDFSSYIRLCFTGEPESVITSSAQVIRNVLLQNGQT
                     S"
     gene            36295..37080
                     /locus_tag="PK937_04410"
     CDS             36295..37080
                     /locus_tag="PK937_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNH31842.1"
                     /translation="MKIGYVQFKPQFGDIDYNAAKIYALLEKKQADLMVLPELATTGY
                     YFRDRKEAYALSEDPKKSFVVEVLVELCKKNGMHVITGFNERNGRKNYNSALLIGKKG
                     IVDVYRKIHLFNTEKNCFTPGDKPLRVHKIASARVGMMICFDWIFPEVTRTLSLMGAD
                     IIAHPSNLVLNYCQNAMITRSIENMVYCVTANRYGEETRPHGDLTFTGQSQIVAPKGV
                     LLHRAPEQKEEAFIAKIDLTKSRQKNMTPKNDLFKDRRTKFYL"
     gene            37117..39039
                     /locus_tag="PK937_04415"
     CDS             37117..39039
                     /locus_tag="PK937_04415"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HNH31843.1"
                     /translation="MNYRILLLWVCVSTTVSAQQVNYEVRFPNIRHHEAEVTAVFSDI
                     SSPVLDIRMSRSSPGRYALHEFIKNVYSVRAEDGRGKKLDVTQNDPYGWQVSAHDGTV
                     RFFYTVYGDHIDGTYLSLDASHAHINPPAMFVYAQNADHRNIRLRFTLPDKTWRIATQ
                     LKESGASVYIASDLAYLMDSPIEISAFQESSWRVPATSGDILFSLILHHTGTPKQTEY
                     LAKVGDGIVREARAVFGEFPVFDQGRYAFMMELTPYASWDGMEHRNSCVITNRDSING
                     DFLDIASTMAHEFFHAWNVERLRPKSLEPFDKARVNMSEELWFAEGFTSYYDDLIITR
                     AGIQSVDRYCKVLTKLMNNVVVDPSKNFGGPAHMSQRAAFTDAATSVDAQNRKNIFST
                     YYWYGATVALGLDLTLRAEHGKTLDDYMQLLWKAYGKTEKPYTSADLESALGDLIGDK
                     KRAQRFFDETIRGNRLPDYKKLLDYAGFVTRAAHADRPTLGQVRLAADDDGILIKDAT
                     LIGDPLYLAGLDRGDKIFSLNDQAVHSIAAWEEVLTQLKPDDSVTIVYEQRGLKGISR
                     VKVAADPSLEIIPVEQTGKPLSEKKSNFRAAWLSSKSTDKPLLYRYCKKCRRAYEFQY
                     EFCPFDGVLLQITPEE"
     gene            39043..>39408
                     /locus_tag="PK937_04420"
     CDS             39043..>39408
                     /locus_tag="PK937_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNH31844.1"
                     /translation="MHLETMKAAVLQVVGSIENLHYSDISKPEPKPGEALIRINAAAL
                     NHRDLWIVFGKYAKIQVPAVLGSDACGTVEQVGDMKDNIWVGRRVVVHPGLFWGNNPK
                     AQGKNYKILGMPDQGTLAEY"
BASE COUNT        10453 a         8587 c         9768 g        10600 t
ORIGIN      
        1 agacagagcc tgatcatgaa ttaacggcag tcatcgccga tgatgaagta ttggcgcgaa
       61 agcgcattcg tcatttgctg gcgaaacgtt cagacatccg tgtggtagcg gaatgcgtcc
      121 acggttatga cgtatgcgat aaggttttgg aatacaagcc ggatttgttg tttctggata
      181 ttcagatgcc ggagatggac ggattggaag tgttgcgtac gctaggtccg gtcgccgtgc
      241 cgggcgtggt tttcgtaacg gcatttgatc aacatgcgat tcgtgcgttt gaactccatg
      301 ctctggatta tttgctcaaa ccgtttgacg atgcgcgttt ttatgaagcg ctggacagag
      361 ctatacaaat gatcggaaaa caacaactac cggataacct caaacggctg ctacagaatt
      421 tgccgcgtcc ggaagcgttt atgccgcgca tcccggtgaa agacgggata cgaatacatt
      481 ttgttgaaac ggccaatata ctgtggatcg aggcgactgg aaaaaacacc atgatgcata
      541 ccaccgacgg ttcgatcaag actgcaaaaa acataatcga ggtcgcccgg gatcttgatc
      601 cgacgcagtt ttatcgcatt catcgctctt atatagtcaa tatccgcgcg atccgtgaaa
      661 tgcagtcttg gcataagggt gaatacgttg tgatactttc agacgccacc cgacttgtca
      721 ctggacgcgc ttttcgtggc aatgttgatc tgctcatgcg ccgctgatat tcttttcgtt
      781 acctgtaaaa tcccttctat tccgcatttc gaccctttcg ttccttaatt gcaacgactt
      841 aattctgtca tcgtaatcaa ttggtataga ctagggtgcg atatacatcg caggtaatat
      901 caaaccttgc acacaaattt tagaaattaa tatggatttc ataaaaccta accccatcgt
      961 ttaatctaac tgaaggagaa gctatgaaaa cgtttatttg gttatggcta acaataactt
     1021 gcataggatc gacgtcttgc cgtgatcgat cggctggtgt gcaagagccg aatgcactat
     1081 cgcaagacga taaagatgta ctatttcaaa ttcataacgg cgatttggcg ggtgctgaat
     1141 caaaaatcaa cgcgatgctg agtaacaatg cggataacct gcggggtcat tttttaaaaa
     1201 cttcgttata cttccatgcg cggtactttg ccagccaggg cgccagccgt gactccatca
     1261 ctacactctt gctgaagtcc tgcgaagaag cgataacaca ggctgaaaaa caatcggagt
     1321 ctatagagca acaattttac ctcggctcat tttatagctt taaaagtcgt gcgctgcttg
     1381 ccaaacgcga atggacatcg gctttttttg ctgcgcgaac cggtaaaaat ttgctggaat
     1441 ctgtgatcga atccgcaccg ggttatgcgg acgcttattt gcctcttggt gttttatatt
     1501 atttcgcttc aacccagcag gcgtggtggc aaggcgcttt agcgtgggta gcggatttgg
     1561 atgttcccaa aacgcaggcg ctcacgtata tacaaaaaac ttatgatgaa ggattgctca
     1621 ataaaaatga ggcgcatttt gtcttaaaca acatcaattt attttttgaa aataagttag
     1681 atatagccga atccctgtta caaaattata atgcccaatt tcccaataac cgtttttttt
     1741 tagcgccgat gcaaggttta aaattaactc gtattgcgga agaaaaagga ggggagttct
     1801 tactcactca tttagacagt ttagtgacag cgcatcctct caatggtatg caaacattga
     1861 atcgcgtggg ttaccggtat ttggggcaaa aaaaattccc tgatgcattg gctgtctttc
     1921 atgctaatgt aaaattgttc cctgggtcgg ccaactgcca tgatagtttg gccgaatgct
     1981 actatacgat ggggcaaatg caggaatcaa aaaaatatta cagtcttgcc cttaccaaac
     2041 ttagcggtga tcaaacaata aatgaggagt ttaaaaagac attgaaagaa ggtattgaag
     2101 tcaaactaat agatttggca tcatattgag gtgagatgaa ataggtataa aatacaaaat
     2161 cccgttccaa aggcgggatt ttttgtggac gcgactggac tcgaaccagt gacccctacg
     2221 atgtcaacgt agtactctaa ccaactgagc tacgcgtcca taatacattt gtgctcggaa
     2281 ccggaatcga accggtacgg gctttgaggc ccgcaggatt ttaagtcctg tgcgtctacc
     2341 agttccgcca tccgagcttt agacacacgc tggaggcgaa ggacagaatc gaactgtcga
     2401 tggggctttt gcagagccct gccttaccac ttggctactt cgccatcttt tcaaagacca
     2461 caaaaaaata accagagccc cgataggtcg agaatctggt tactcatttc acttactcta
     2521 ccgttgtagt aatcacaacg gatcccatct ggattacacg aacgagagcg agagacgaga
     2581 ttcgaactcg cgacatcaac cttggcaagg ttgcactcta ccaactgagc tactctcgca
     2641 tatatatgaa caaaccaaat gcctaattca aaactgggac agtaatatag ataaaaatat
     2701 ttcccatgtc aagaaatttt gaataaaaac aaaaaatgat ttttaagtta aataaataca
     2761 tatatttaag cgtgtcattt ttttaaattg acacgctcat gtaagtcaaa atgtcatgac
     2821 ataagtggcg aatatgatgt tgtaaacaag ttatcaacat gattgatgtt gtttttattg
     2881 aaactgaatt ggcttgcata acgtatcatg tttttattaa tttatcagcc attatgaatt
     2941 ctaaaaaagc attaataatt gtatttgttt gggttttcgg gctaagctcg tgtgcattat
     3001 ttattgatcc atatgaggcc caatatcgcc agttactcca tgagcgggat tcacttcagc
     3061 tttcgatcgc tcggactcgg catgatctca aagatcttga agcggagctt tggaaaacgc
     3121 tgtatgatac ggagtacttg cgtaacgcat tggttttggc tcaggccgat tccgtaaaaa
     3181 atttttacta taactacgta tttcgttatc gccgtccgat tcaggctatt gcggaaattg
     3241 atcccgcttc gccatttctt tttccgctta aaaccgaaaa agaccaaaac cggattaaat
     3301 ttcgcatcat gtggggacgt ttgttctact ttgactttaa tcatcttggt attgatatcc
     3361 gtgcactcga aggagatacg gttcgtgcca tatatgacgg cgtcattcaa aactatggcg
     3421 gtgccgaggg atatggcgaa cttgtagccg tggtcgaaca tgaatatcgc ggtctatgga
     3481 ataaagacgt agtgccgcaa cgattcatgt ccatatacgg gcatattcga aatcgttgtt
     3541 atgatggcga gccggaattg aaatggaaag ccggagaccg catccgtaaa ggcgatatta
     3601 tcgggtttat caatgatgac agtcgcaatg ggtatggaat ggagcatttg catctcggta
     3661 ttcggcgtca aagcgcgatg gatgcgcaag cggtggacgg aggatattgg atgcgcggat
     3721 acgacacgcc caaaaacagc aaatttcagt tttttatgaa tccgatagac ttatttggtc
     3781 gctatgttca gtttgttctt cctgaatagt cagatttctc tttgcatttt atagataaat
     3841 tatctattgt tgccaataag taattttgct tgccggtttt ttgtttttca tggaggattt
     3901 ttcgaatgcg tctgatcacg cgtaccgatt tggatggcct tacatgcgcc gtctttatca
     3961 cgatcatgga aaaaatagat gaagttgtat tttacgaacc caaagaaatg caggatggta
     4021 aagtacctgt taccgatcgc gatatcatag ccaatcttcc ttatcaccct aattgtgctt
     4081 tatggtttga tcatcatcaa agccagctta ttcatcaaaa tgcagaatat cgtggaaaat
     4141 ttgccgtagc gccgagttgc gcacgggtga tctatgattt ttacgaacag cctgacgaat
     4201 taaaaccctt caaagaattg ctcgaggaaa ctgatcgcgt ggacagcgca cagctgacca
     4261 tggaagatgt gatgaatccc aaaggttggg tgctactctc ctatacgctg gacccgcgct
     4321 ccggccttga tgcgtttacg gactattttc agcgtatgat cgggtggatc atggtgcata
     4381 cggttgatga gcttttgcaa ttaccggatg tcaaatctaa agttgataaa tatttagctg
     4441 atcaggataa atttaaagaa gccttgacta catattctcg cgaagatggt aatgtcatta
     4501 tcacggatca acgttccgtg gataaatttc cgtccggcaa tcggtttctt atttatacgt
     4561 tatttcccaa agccaatgta tcggtgcgct tattcaaagg caaagaaccc ggcgtcaccg
     4621 tatgcgccgt cgggcacagc atttttaatc atagctgtaa aacgatagtg ggggagctta
     4681 tgcatgagta tggcggcggc ggacataagg gtgcgggaac atgccaattt ccagaagcag
     4741 aggccgaagc gaaagttgca gagatcattg aacgattgaa gaaaaacgga taatcggctt
     4801 aaaattatat aaaaaggcaa cctgaagaag gttgcctttt tttgtgtcaa attttgcgcc
     4861 ctaagattta tggtgtggtt tagtatcggt ttgatacgtt ggtttaatta aaaactaatt
     4921 gccaacgaac taagaacgga tagattagaa gtttcaccgg aaaaaatagc atcacggctt
     4981 tgtaactggc gcccttctat tcgcaatacg gattgtttgg tcaaagcata atccagattg
     5041 gctgatagcc ccaatgtttg gaatccgtgt acagtaccgg ttgaaacgat gacctgattt
     5101 ttatccaaga attgctcggc gcgcacagca acgctccaca tgtcattgag catataccgt
     5161 gttataatag cccatgtcag ccatgtgttg tattttgaac ttcctttaga tttctgctcc
     5221 gcaccaaaat caactcctgt agtaatgcct aattttgggt tcacctgcca aatagcgtag
     5281 gcgttgtcga aatagcgcca ctgacgcgta ctgtccgcct tatcattacc tacaaatgtg
     5341 ctataattaa tggttaatcg atctgaaggc ttgtacacga tctgcgtgcc cattgccgga
     5401 gttacgtttt tagaagaacg gtaaattcgt tgccaaccgt tacacaccac agcggcaaag
     5461 gtccattttt ccgataagga atatgttgcc ttgacaccgc taagataata cggcgaattt
     5521 tcggcggcca gtgaacgcga taatgtccaa ttatcttttg aaacagcgct ttcccaacct
     5581 atatgtgatc caaataaacc gacttcaagg gaggtctttg cgttgatttg tataccggta
     5641 taagcctcat aaagatgctg gtaggttgcc ggttcagcgg aaagattgga ttgagcataa
     5701 gttccggcca tgatgccgac gcgtgcatac gcttcatcgt atttagcctg cactcgtaac
     5761 aacgccagat ttacgttaaa ttcattgtgc cgtgtatgat tataaagaaa cccgggtcta
     5821 tcgccggaga caggctttga aaagtcgtat ccgtagtatg cgtccacgaa gcccgaccat
     5881 gaccattccg gaatgttatt ctgagcccat gtcggcagaa catggatcgt aatcatgaaa
     5941 caaaataaag aaaatcgcat aacaaaacct ggttttttgt atgaaaaact attttttttc
     6001 catcactgtt ttaagattag caagaccggt agccaagtca tcgccgatca ttttttccat
     6061 gtccataaag agcaagaaaa gattcatcgg ataatccatg tgcccgctaa agccccactt
     6121 gaccagcgtg gtgctgtccg tagctgcact tgtggtcata taagccggtt ctttggattg
     6181 ccacggttca aaaaaacgca attcaaaatc aatgcgttcg ccatcagcga tttttacgat
     6241 ttcctgttca cctttaccga catccgggtt ttgactttcc catgccgaca caaatccaac
     6301 ctgcccgtct tcaccgcgat aggttttttt catgttggca tccattgtag cccatttgct
     6361 gtagtggtcc tgattcttaa gaaactttac gtaatcaaat acaacctgat tgggttgatt
     6421 gatcgtaatg ctacgttcga cagcatactg ctttttcaca aaaagcgcta ccagaagcgg
     6481 caacgcgatg aggatgccca atacgatgag caacagacga agtgctttca tgagaacctc
     6541 cgtgggtagg gttttagggt aaagaatagt acggttaaat acggaatgtc ggatattaag
     6601 ttacgtcaat atttgctgcg aaagagccaa aacaaaacag agagaatcac gctcaacagg
     6661 atgccggacg tgatcggaaa ataaaacgag aagttttccc gttgaataaa aatatctccg
     6721 ggcaagcgcc cgatcagagg gattttatcg gaaaaagtaa gaaacattcc gacggcaatc
     6781 aaaacaccgc cgaggatcat caatgttttt ccgaaatcgt gaaatggctc catacgatca
     6841 tcgtttaaag gttcatcttt tcgacgacgc gtttcatgat cagtgtcatt ttgggttcgg
     6901 cttcaccggc ggttttgatg attttgggca gatcaaccgg ttgaagcgaa tcggggaaac
     6961 actcatcggt aataatcgaa aatccgagac aaggaatacc catatgatta gcgacgatta
     7021 cttcgggcac agtagacata ccgacaacat cggcgccgat ataacggagg aaacgatact
     7081 ccgcacgggt ttcgagattc ggacccatca cggcgacgta aatgcctttt tgaactttaa
     7141 ttttttcttc caaagcgatg ttttccgcta aagcgatcaa tgtacgggaa tagggttcgc
     7201 tcatatcagg aaaacgcgga ccaagcgtat cttcattttt tccccgcaac ggattatcac
     7261 cgagcagatt gatatgatca tccatgatca tgatatcacc gggttggtat tgaggattca
     7321 tgccgccgca ggcattggat acgagaagcg ctttgatgcc gaggaacttc atgacgcgaa
     7381 cggggaatgt gatctgttgc atggtatagc cttcataaaa atgaaagcgt ccttgcatag
     7441 cgacgacgtg tttgccgcca agtgtgccga agatcagctt accggcatga ctttcgacgg
     7501 tgctgagagg gaaatgcggt atatcctgat agggaatttc gatttctttg cgtatctcct
     7561 gtaccaagcc gccaagcccc gtgccgagta taatcccgac agacggctgt gttttggctt
     7621 tggattgaat atagttaacc gcttctgcga tttgtgctgt caaaggactc atgatttctc
     7681 cgaacgtttt ttctctaatt ctttatgtat ctgatccaac agttcatggg attttttatc
     7741 cgcagaatcg ccgagcattt tttcgaaatc cgatttttta aaaatacccg tagcattgtg
     7801 cgtaagcttt tcgagttctt cgatcacttt ctttttcttt tcgtggcgtt ccgggtcagt
     7861 aatcgccgta tcgtcagccg attcgcggat cgccgcggct aactccggag gcaggtcatc
     7921 cagtttgaga tcctgtacaa acgaaccgat accttcttcc aggccggcct tcagttcttc
     7981 ttccgtggct accgggatga tgggtggagg cagttcggtt tccttaattt cgacgggttt
     8041 ttctacgcgc ggctcgacct tttcgggaac cggtattgtt ttagcagcgg cttcggcctt
     8101 ttgatcaccc acgatttcca aaagcgcaat ttgagatttc aggtattcgc gcaaatgcgc
     8161 ggccactttg gttttttgca gctgaaggaa attgacttct tgagcgagtg attcattttc
     8221 cttctgcgcc tgcgtccgga tgcgttcggc ttcggcgcgg gcctgttcta aaatcaaatc
     8281 cgcatccaca cgcgcatcca taagtttttc gactttgacc atttcctgtt tgtaatcatt
     8341 aagacttttt tcactgcttt ggatgcgaag gcgcaagtac tgggtttcgt cttgaatcgc
     8401 tttgagcttt tgttgaagta atttagtctc gtcgagaatt tctttgtagt tttcagcaac
     8461 cacttcaaga aaagccgtca cttcgtcggg atcaaagccc cgcatgacgc gtttgaattc
     8521 ctgatgttgt atgtcgccgg gattgagttt cacagcgttt cctccgattt aggagcggcc
     8581 gatttggcgg cgcgcttgcg ttttccgaaa agggccgtac cgatacggat atagttggcg
     8641 ccttcttcga tggctatttc aaaatcactg ctcatcccca tggagagcgc gttcatttcc
     8701 acgttaggta tattttgttc gcgaataaaa tcgcgcagtt cgcgcaagcg gcgaaagtaa
     8761 ctgcgaattt tttcgggatc attggcctgt acagctgtca gagcgccgat cgtcatgagt
     8821 ccctggatgc gaatattttt caattcagaa acttgtttga tcagctccag cgtgttatcc
     8881 ggctcgacac catatttggt ggcttcgcgg gatgtattga cctgaatcag gatcggcata
     8941 gttttatcca ccgtataagc gcgacgatca atttctttgg ccaaacgaaa cgaatctact
     9001 gaatgaatca tgtcaaacag ttctacggct tgacgggctt tgttggtttg caggtgacca
     9061 atcaaatgca gttggaagtc cggtacattg ccggactgtt caagttctgt atatttctct
     9121 ttggcttctt gaaccttatt ttcaccgatg agagaaatgc cggcagctac agcttcgcgg
     9181 acataatcaa tgccgtatgt tttggtaacg gcaataagat gaatttcttt agggtcacgt
     9241 ccaaccttgc gtgcggatgc ttctattttt tttcgaatcg tttcaatatt ggcgcgaatc
     9301 gtcgtttgca atgctatatc cataaatcaa gttcctttaa tttctatttt cggccaaaac
     9361 cggctcgaat gcgcaattcc ggttcggttt cgagtttaat acgttcaatg ccgcttatag
     9421 ttttacctaa aaactggtgc agcaaattat cacccatata aaggaccgat aagattattt
     9481 tttcgtgtgc aacctcaatg ccgatactgg aaaatgtaat gcgtttgata aatttagtca
     9541 gatcgggatt tttttctacg gcatgcaggc gttccgccag cttcgcgtca atgtgaaact
     9601 tttgattgaa tacctgatac gaatcaccgg tcttggtgaa acggaattct ctgtcggtaa
     9661 tcgcgtcagt caatcggcgg ctaccttcgg aaataactga aagcacaccg ctgccgtcgg
     9721 tcgtaaaaac tttaggcgtg ccccatttat cagctaatat caatccgacg caattataat
     9781 cttcgatgag ttttttaaaa aaaagctcaa tcgcaacatg gggaacggta ctgcggtttt
     9841 tgcgcgcctc aaaaaggcgc gccacattga gtacatcttc tttgaccttg cgcaccgcgc
     9901 aggcgggttt gagatgaata catccttcgt gcaggcaaga ctcgaccggt tccacacagc
     9961 cgatacgctc tttgtagtcc aaaagatttt cacagcgcgt acggacggtg cgttccgtcg
    10021 tcgtcacacc gtgtttttcc tgataataca atataccgtc ttttatcaaa gcatcgagga
    10081 gattattcaa ctgatgataa ttaaaacgat ccagcaattc tctaaacgca tccggttgca
    10141 gtaacgacag atgaatgacc tgaatttctt cggaattaaa tacctgcggg tccagcgcga
    10201 ggatgcgctg cgagcgaaaa accgaatcgg gcgtttccgt catagcggaa gctgccggag
    10261 atatatgctc tgctgttgac atagaaaaaa caatacgatt catcgtttga atacaaaaac
    10321 aaagataggc cgtatatatc tgaaaatcaa ccaaaatgcc tatttgtatg cttgcgatgt
    10381 gagaacgaac attcatgcgg tcattgtaaa acaggtattt agacgtttca ggcgtttttt
    10441 tggaagtatg aagtgaatgt ttaagatgct attttgtatg cggtgtgttc ggatttatat
    10501 ccgtggaata ataccctgtt gatgattaaa cccgtttttg tgttgcgtga tgcgattgta
    10561 ttgattataa ttgcaaagtt ttagtgttaa ccgatggaag aaaaaactat gaacaaacgt
    10621 tggatgattt tcgtcgtatg gtgttgtgcg ggcgtactgt cagcgcaaca ataccaggtt
    10681 cgtatttcgc cggatacgat tttgtgcagt gaccaagaag cgatcatacg tgtatcaccg
    10741 ggtcgctccg gtgtcgagct cacggccgtt gcccgcaaag gcggtggttc gatcggtgat
    10801 tttcgatatg ctaacagtga ccaggggtat gtcaatcagg cgattttttc gagtcctttt
    10861 tccggggaaa tcgaaatcga agtaatggat aataaaaaca acaaagtcgg aatggtaaca
    10921 gtaaccgtgg ttccgccgac gatggaattt atggaagacg aatccatcgc ggaatcgaat
    10981 gccgctgaga agcgcattcc gctgaagatc aaagttttgg atcagaacgg tagttttatc
    11041 aaaacggcta aagtgacttg taaagtatcg gaagtggaag gcaccggaaa aaataaaaag
    11101 atcaaaccga gtgatgctaa agtcggggcg tttgttttac tggacggata ctacgaaggt
    11161 cagttgacca atcttaaaaa tgccacatac cgtcttgaat tctttgatga agcgcacttg
    11221 ctgccttttg acagcgaaga aaacccggat gcacaacatc cgtcgcttct gattgattca
    11281 tggccgatca gtttcaattg accaacgaaa aattatgatg aatacacggc cccgtgtatg
    11341 tgggaaaaac ctgcaacacg gggtttgttt tttgtgaaaa gagaaattac ttattttata
    11401 atccgattag ccgtttctat tcctcaattc gcccatagga ttaaaatttt gagtatgatc
    11461 aaaccgtttt ctttgagatt agacgaaccg cgcggatggg gttttgtttt attgttgtta
    11521 tgcggctgtg cgacgatcgg tgcgcccgga ggggggccgg aagataagga accgccgacg
    11581 gtgataacga cgtttcctgc caaagattca accggagtat cccgtcagac ggaggtcaca
    11641 gtgactttct ccgaagcgat gaataaagct tcggtcgaaa aagcgttgtt tattgcaccg
    11701 ttcccggcac gtgatccgga tctgtcgtgg gatgatgcga cgttgacgat tcgttttaaa
    11761 gacgcgctgg aagaaaaccg tacctgcgtc atcacgatcg gcacggacgc tatggatgca
    11821 catcagaata aatttaaaag cgcatggagt tttgcttttt cgacaggtga taaaatagac
    11881 gccggttcta tcgccggtac ggtcgtatcg gaaagcaaaa ctaaaccgag tgtatgggcg
    11941 tacagactca acggtcatac atcctcacgc gacagcatga tttatactaa acgagccgac
    12001 tatataaccc aagtggatgc acaggggaaa tatcgttttt catatttggc tgatggagcc
    12061 taccgcattt atgccgtatc cgatcaaggc gaagatttta ggtatcatcc gatgcaggat
    12121 atgatcggtc ttgcgccgcg cgatgtgata ttggcggata catcaaaaga agcgttgttt
    12181 gtggatatcg ccgtatttag agaagacacc acgaccatgg ctgtacaatt ggcacaaatg
    12241 accgggccta atgaagtttt gattcgcatc aataatgttt tggctgaaag ccgatatgac
    12301 ggtaccaaaa ttggttcaga acatattgct tcgcattttt caattcgcga tagcctgagc
    12361 ggtaccgatg tagcaatacg ggatgtatat tttgtaacga cgggcgcaac tgaagaaatt
    12421 cgacttttat gcgacagtat gtcttctcaa cgcgtgtatg ttctcgatgt gcgcggtatt
    12481 tatggattgt ccggcgactc gattgccttt caacaaatta attttcgcgc ggagggtggc
    12541 tttgaaacat tgcgtgccaa gattgaatgg acttttccat acggtacgcc ggggttgatc
    12601 gcagttaatg attttatcgg atggaaatcc gaccgcggac tggatcgtcc gacgtgggaa
    12661 aaggcttttg tgttgcgtga tactttaggt aactcggtaa acggaacttt tttttggatg
    12721 cattcggcat ccggaaaatt cataccggcg cgcaacctcc gggggcgtat gccgtatgag
    12781 gtgcatgtgg acggtaagat attacaagat tggtccggtc gcccggcgtt tgacacgacg
    12841 atggtttggc gtttggtcgc catgccggaa gacacggtcg gcacggtatc gggtatgatc
    12901 gcggacgaat cttccgatga tcgcgggatg taccatataa cggcacgtaa tctcaatcgc
    12961 agcgggcgcg attatacatt gatcgtacca tcgccggggg gttttacttt ggacgggatg
    13021 ctgcccggaa agtataattt tcttgcattc cgtgaccggg atagtaacgg cgtattttca
    13081 ttcggccaaa cagcgccgat catccgaccg gagcgattta caagttatag cgatacggtg
    13141 acggttcgta aaaattggga aacatctaat attcaaattc gtttcaggaa ataagacatg
    13201 aaaagcattt attatttaca tcagatcaaa accggaaaaa attttcgcgt ggaagaaaat
    13261 caggaagtga ccatcggtcg ggcatttgac aatacgctca tgattgacga taactccgtg
    13321 tcgcgctatc atgcgcagat caagtggaaa aaaaacctga tgtatattac cgatttacaa
    13381 agtacgaacg gaacttcggt gaacggtgaa aaagtggaac atggttattt tacagaactt
    13441 aacttctccg atttggtgaa aatcggcaat attgaactga aaatccttga tgaagaatcg
    13501 gtcatcggca aaaactttac ccctgatcag gcaccggcca aaacgatcgt aatcaatcaa
    13561 gcgatgttaa aaaaagctgt tgatcccgat gattttacgc acaattaaaa atagagggcg
    13621 gtagatgaga ataaaactct cgttatgggt ggtaggtatc actttgtcgg tgatgatcgg
    13681 atgttcatcg cgcacggctg tacaaccgga cattaaaaat aatacgacgg cgtccgcttc
    13741 gacagacgac catggccata aaacagaaac tcttgcggaa gaaaaaaagg gaccttctga
    13801 acctgtaatc accgttgttt ctgttccgaa aaccgtgctt tctgccgaag aaaaaatgga
    13861 gcttcgtgct gtgggggatt cgctgtacga aaaaggtgat tttgaagaag cgctacccgt
    13921 gtgggttaaa atactggagc atgccgacga caaaccgacg atagcggcgg cgcactatgt
    13981 gcttggtaca ttgttttatc agcggcagga atattttaaa gccgagttag aatttaaacg
    14041 cgcggtcgag tcggattcga ctttgataga cgtgtatcgt gagttgggct ttgtctattt
    14101 tgtcaaaggt gattacgaca aagcacgggc ctcatttgaa acgatgctaa attatctgcc
    14161 cggagattcc gaagccgtac attgggtaga ttatacgatc ggcacgcagg cttacgaaga
    14221 aggtttgaaa ttatttaccg atgaatcgta cgataaagcg atagaaaaat ttcaatccgc
    14281 gattgcttat cttgaggagg acacatcggc taactataag attcactact ttatcggtaa
    14341 atcgtatttg gaaaagctgg actacgataa tgcattaaaa catttgagca aagcggtttc
    14401 gttgcgtccg gaaatgcacg aagggtacac cgaaatcggc gcgatctttt tttcccgcag
    14461 tgattatccc aaagcgatcg gatttttcca acgcgcattg acgtatcaac catcatccag
    14521 caaagcactg aataatcttg gttatgtgta ttttactctc ggtaatcagg ctttggcggg
    14581 cggtgataaa aaccgttcgg atgagtttta taaaaaagcc gtcacctatt ttgaacaggc
    14641 tctgatcgct gatccggcga tggcgggggc gcgatacaat ctcgatcatg tacgtaaaat
    14701 cttgagcggt gcgcgcaaag cgacggcgta cacgctattg caagccgcat taaaaacgga
    14761 aaaccatgcg gagcgcattc gcacatacta taaagctatc gaacaagatt cgacctacga
    14821 tgatgcgtat gtcaatctcg gcgtagccat gttttatcat ggtcaggccg attccggtat
    14881 gattatgaca gagaaggcga ttgcgatcaa cgcctacaat gctcaggcgc acaataatct
    14941 cggctatatg ttgggtacag cacaccgctg ggaagaaagt ctgaaacaca tttttctggc
    15001 cattcagatc aagcgggatt ataccgatgc ctatgtcaac ctcggttatg tttacatgtg
    15061 gaaagaagat ttcgagtcgt cccgcaaagt ctggttgcaa ttgctgaaaa ataatcccga
    15121 caatacgatg gcgcgtaaag gtttggccga aatggaacgc cgcgaaaaaa tggtgcgttc
    15181 cggcgaaacc acaacgcata ttgagataaa cgaagatcgc gcgggcggcg gttcaaatta
    15241 aaatacatgt cgcgacatgg tatattcttg ttaataaata caaatttatc gttttcttct
    15301 tggcctaatt aacgccgaca tgcgtaccga ttaaaatgtc cgaatctaaa acaatacgtg
    15361 ctttagctgt tcacgccccc ggtgcgctac tttcttcttt tgaatatggc cgaacgtctt
    15421 taggcgccga tgaaatcgaa gtcaaagtcg aatactgtgc tttgtgtcac agcgatttgc
    15481 atctgatgga tggtgactgg gcatgtgctt ttcctttcgt accgggacat gaaatcatcg
    15541 gatctattat tcaaaccggg gtttccgttc gagatctgaa gcccggccag cgtgtaggta
    15601 tcggttggca atgcggttcc tgcgggatat gcgaagtttg tatcaccggt cgtgctcatt
    15661 tgtgtgaagc cggaaaaaaa cgaacctgcg ttaatcaata cggtggattt gccgaatacg
    15721 tgcgagcgca tcagcatttt gtttatgcca ttccggaaac gataccgtcg attcatgccg
    15781 cgccacttat gtgcgcgggg cttacggttt ttagcgcctt gcaaacgtgg gttactccga
    15841 aaacacgacg catcgggatt atcggtatgg gcgggcttgg gcatcttgcc gtccagtttg
    15901 cggcgcgtat gggatgtgaa gtcaccgcgt ttgatctgtc tgaggaccgc cttaccttag
    15961 cgaaagaatt aggcgcacat caggcggctt taacagactc tgcaccaaag ggtacgtttg
    16021 atctgatgat cgcagcaacc gccgcttcgc tggattggaa tcattggttg cggcatttga
    16081 cctggggcgg cgtattgtgt ttgttaggtt atcctgcgga ggaaattcgc atttctccgg
    16141 atatactgct tgacggccag aagattgtaa ccggcagtgt ggttggaagc cctgagacca
    16201 tgcgaacgat gctggatttt gccgcacggc atcatgtacg tccgatggtt gaagagctgc
    16261 ctatgaaaca ggctcacgag gccgtcgcca gactgcgagc aaataaggta cgctatcgta
    16321 tggttctcaa agccgactgg gaatgatcag gacttttgct tgccattcac ctcaaaccct
    16381 cttattttca cccgcatttt tttgaaacat gctaccatcg atgaatcttt ggtaaacgat
    16441 atcactttaa cgtgaatgaa aaaaatatga tatatcccgt gattatggcg ggcggatcgg
    16501 ggacccgatt ttggcctcgc agccgggcgc atcgtcccaa gcaactgctg aatattttcg
    16561 gcgaacatac gatgatacaa catacggtaa accgtatcgc ttccatcgca aaaccgtctg
    16621 aaattttggt tgtgaccaat aaggcacagg catccgaagt gcatcgccaa ttaccgcaat
    16681 taccttcgtc gaatattctc gtggaaccgg tgggtcgtaa tacggcaccc tgtatcggcc
    16741 ttgcggcgct ccatgtgatg gaaaaagatc cgaatggtgt gatggtagtt ttagctgcgg
    16801 atcatcttat cacaccggat gacgtatttt gtaaaaccgt cgaagccgcc gctgaagtag
    16861 cgcttcgcac ggaggcgtgt gtgacgatcg ggattgtacc tacgcgcccg gaaaccggct
    16921 acggttatat tcaatttata gaaaacgaat cgctcgaagc ccacggtgaa aaagcttatc
    16981 gggtgaaaac ctttgccgaa aaaccgaatc ttgaaacggc gaaaagcttt attgcgaccg
    17041 gtgatttttt gtggaatagc ggaatgtttg tatggaaagc ctcgacaata ttacgattga
    17101 tcgaaatgca tcttccggaa ctgtatgaag gccttgtaga aatcagaccg acgatcggta
    17161 cagcggatta tacggcaaca gtagatcagg tatatcgtcg tatcaaaggt atatctatag
    17221 attacggcgt gatggaacac actaaagaag tttatgttct caaaggtcat tttcgctgga
    17281 atgacgtcgg aagctgggaa gaggtgtatc agctttcaaa taaagatgaa aacggtaatg
    17341 cggtcatcgg tcaagccgtt tgcatcgaag ctaataattc attattattc agcccgggaa
    17401 aaatgatcgc gcttgtcggc gttagtgatt tgatcgtcgt tgaaaccgaa gatgctttga
    17461 tgatctgccc gcgtgatcgt gcccaggatg taaaaaagat agtagaaaca ctacaggcgc
    17521 agaaaaaaaa cgaactttta taagtagccc atgaaaaaag aaaaaatact cgatacgtta
    17581 gaagatatag cggcgaagct aggactgaaa gtacggcatg aaaaaggcga ttttgccgga
    17641 ggcctttgcg tcaaagatga tacgcagtgg attttgctta ataaacgtca catgattgat
    17701 cgtcggatca atattttagc gagagaattg gctcctttcg atcttacggg tatcgaagtg
    17761 cctaagccgg ttatgaccat catcgagagc gagcgcaaac gcgcaacaca ggatgaattg
    17821 gaattgacgg attcggaaga atagccggaa taaaattttt catttttatg aaacctcttt
    17881 gtttaaacga agaggttttt ttgtccgttg attcaaagcg gcggtttttt tatgtattcg
    17941 atgagcgtac tgtgctggcg tagcgcctgt ggcatatcgc tccaaaaaaa tgtataattg
    18001 cagccatgaa tgatacactg gaacataaac tcgaacactt acccaaaaca ccgggatgtt
    18061 atctttttaa aaacggtgag ggtaagatca tatatatcgg caaggccaaa attttaagaa
    18121 atcgcgtgcg tcagtacttt caagaaggga aacgcgatga tactaaagta gcggtcatgg
    18181 tttccaaagt ggttgatctc gaggtcattc aaaccgattc cgaaatcgaa gcattgatcc
    18241 tcgaatccaa tttggtcaaa gaacataagc cccgttacaa tatcgaactc aaagatgata
    18301 aaagttttcc ctacatcaaa gttacggatg agatgtttcc ccgcatctat gtaacacgcg
    18361 aaaaagaacg cggcggagga aaatttttcg gcccatatac agatgtgaaa aatttgcgac
    18421 acacgctcaa aacactccat cgcatttttc cgattcgcag ttgccggcat tatttcactc
    18481 cggaattggt ggagtccaaa aagatcaaac tgtgtctcga ctatcatatc aaaaaatgcg
    18541 acggaccgtg ccaagggtta gtcacaaaag aagactatcg cgctgtcgtt gatcaaatga
    18601 cgcgtttcct taacggcaaa accggcgagt tgattcgcca actgaaagtc gccatgcaat
    18661 cgtatgccga cgcgatgaag tttgaagaag cggcgcgttt acgtgatcgg atcgcatcta
    18721 tcgaaaatta tacggccgcg caaaaagtga tgtttgaaga tatggccgac cgggacatag
    18781 cagcggtagc gatcgaagcg gatgatgcgt gcggcgttat attcaaaatt cgagacggaa
    18841 aactgatcgg gcgtcagcat tattatttca gtcacgtcac ggataaagcg ccggaagaag
    18901 tgctcgaatc cctgcttcaa ggatattata tgcaggcgga ttttgtaccc cggcatattt
    18961 atttaccgca ggcgctatcg gaggaaactg cactgaccac atggctaatg caaaaatccg
    19021 gggaacgcgt cgaattggtc gtacccaaaa tcggtgaaaa acacaaactc gtaaccatgt
    19081 gcgagcgcaa tgcggaactt cttttaggcg agctcaaact tcagcgtctc aaagccaaag
    19141 agcaatttgt accgcgcgta ctcgagagcc tcaaacgcga cctgtcatta caacgtattc
    19201 cgcggcgtat cgaatgtttt gataactcca atattcaagg cacggacccg gtggcttcca
    19261 tggtggtttt tgtagacggc aaaccgaaaa aaagtgatta tcgaaaattt aaaatcaaaa
    19321 cggtggaagg cccggatgat tttgccagta tgaatgaaat catcacgcgt cgttatctgc
    19381 gtgtactcga ggaaggtttg gatttccccg atctgatcgt ggtggacggc ggaaaagggc
    19441 agttatcggc tgcggtaaaa gcactggaag cgcttggcat tgtcgtttcg cgcgcgggat
    19501 cggaggggca ggccatcgtc ggtttggcca aacgcctgga agaaattttc ataccggatg
    19561 tgcacgatgc gatcatgatt cccaaaacgt cctcggccat caaacttcta caacatgtga
    19621 gagacgaagc gcatcggttt gccattacat ttcaccgcag tttgcgcgat aagcgcacct
    19681 tggtatcgga aattgatcag atcgaaggtg tcggagaaaa gcggcgcacg ttattgctac
    19741 agcactttgg ttccgttcaa gcgttatccg aggccgggct ggaggaaatc atgctggttg
    19801 aaggcattcc cggcgatgtg gcacggcaaa tctatgcgta tttcgaaagc cgtgacgacg
    19861 aagaaagtca ttgaagaaag gcctctgacg ctgtattatt tatataatta accttaatcg
    19921 gaataaacac aggtatgagt gaatttttag aaactctcgc taaacgggta ttggtatgtg
    19981 acggagcgat gggtacggtt ttgtatgaaa aaggaatttc atttgatcac tgttttgatg
    20041 agatcaattt atctcatccg gaaatggtac gtgccataca tgaggcttat attgatgccg
    20101 gggcggacat tatcgaaact aacacgtttg gcggtaatcg ttttcgtctg atgcatcacg
    20161 gcttagaaga gaaaattcaa gctgtgaatg ctgcaggggt tcgtattgcc gtgacgacgg
    20221 ctaaatataa aacagaagcg ttgggtcggc gtatttttgt ggccggttcg atagggccgt
    20281 tgggtaaacc gctggaaccg atcggaaaaa taacgcatgc cgaagcgcgg gcttattttg
    20341 ccgaacaagc caaaattcta gccgataacg gcgccgatct tttgatgatc gaaacgatcg
    20401 gtgatcttga agaagcgcgc gaagccgtca caggggcacg tagtgtcacg ggtcttccca
    20461 tcgtcgcgca aatgacattt aacgatgaag ccaaaacgct gatgggaaat aaaccatccg
    20521 aagtggcgcg tgtactcaaa gaagcgggcg ctaatatcgt gggcgccaat tgttccgtcg
    20581 ggccgcaggc gttactggaa gtgctggaaa aaataaatat cgttccggat atcggatttt
    20641 ccgttcagcc caacgccgga ttgccgcggc tcgtcggcgg gagatacatt tatttggcgt
    20701 caccggatta tttttccgat tacgccaaac tttatatgca agccggagcc aatattattg
    20761 gcggatgttg cggtacgacg ccggagcata ttcgtcaaat caaaaaagct atcggcgatc
    20821 agccgccgaa aaaaatcgaa aagaaaacgg cggtcatcga aacggaagaa tcgtccaaag
    20881 ccgagcaacc tccatcgcat ctacgttcct cgctcattga taaatttcgc gataaaaaat
    20941 ttgttatcag tgtggaattg gatccgccgc ggggcctgaa ttatgataag gtcatcgaag
    21001 gtgcggtaat gtgccgccgg cataatgtgg atgccatcaa tattgccgac aatccgttgg
    21061 ctaaagccgg aatgacgcct ttggccatgg ccgggttgat caaacaaagc gttcagatcg
    21121 aaacgatatt acatttttca tgccgtgacc gtaatctgat cgccatgcag tccgaactga
    21181 tgagtgcgca cgtcatgcac attcgcacga tactcggcat taccggcgac ccgccgttgg
    21241 tcggtgatta tcccaacgcc acaggggttt tcgatgtgga ttcgatcggg ctaattaatt
    21301 tgattcgcaa tctgaataac ggcattgatc tcgccgggaa atccatcggt tcgcgtacta
    21361 atttttttaa gtcgtgcgcg gttaatccga cagccgtaga tatggcgcgc gagtatgacc
    21421 ggtatgatca aaaaatcgcg tcgggagcgg actttgcaat gacacaggtt ttgtatgatc
    21481 tcaagccact ggaagatttt gccaaaaaat acaaaggcaa aatcccggtt cttttgggta
    21541 tcatgcctct aaaaaatgcc aaacatgcga atttcatgca tcatgaaatt ccggatatta
    21601 cgattcctga aaatatccgg gaacgtatga ctaaagccgg cgaccgcggg caggaggagg
    21661 gcgttttgat agccaaagaa tttcttcgcg aagcgaagtc tatggtggac ggcgtctaca
    21721 taatgccgcc atttaataaa tttgaaatgg cgttcgatgt gctggaagtt ttgtaaaata
    21781 ctatatctat gaaacccaca gaaatcaaaa cagagatagg ccgctccgaa gcaacccatc
    21841 gccagtcatc ccggggtgca ttgtggcgta atatgctggc ggccatgcgc ccgttgcatt
    21901 gggtaaaaaa tatttttgtt ttggtaccgc tgctgttctc ggcttcggaa tggacgtctg
    21961 ttcagatagt gtacatttcg gcagcctttg ggttattttg tttggcggcc agtgccgtat
    22021 atctttttaa tgatgtggtg gatcgggaag aagatgcgcg tcatccgtcc aaacagcttc
    22081 ggcccataac gcgcggcctg gtatcggtgc atgatgcgct ttttatggcg acgatactgg
    22141 cgttcgcttc catcggggga gcgtatgcac tcgcaccggc gttggcctat gtcatagcgg
    22201 gatatgtggc gcttaatatc ggttacacct ggtttttgaa acgatgggtg atcgtggacg
    22261 cggtggtcat cgctttgggt tttgtgattc gtatttgggc gggcgctgcc gctgtgaata
    22321 tacccaccgg gacgtggatg ctggttacta ccttttttct gtccgcgtta atcgcattta
    22381 gtaaacgccg ttatgagatc gtctatgacg gtacggaagc gtttcacacc cgcggttata
    22441 caccgtattt gttagacatg ctgattttat tatcggccgc gtcggttatc ggttcatatg
    22501 ttttttatat cttggcgcgg ggaacctggc aacacgaata tattatactc tctacggtct
    22561 tggtcgtgtt ttacggcacg atgcgttata tctacgtaat tcaccgcaat gaggaacgcc
    22621 tcgatcatac ccgcttaata ctctcagatc gtcccttaat ggccgcagtt attttatgga
    22681 cggcgctgat gatggtcgct attcaccgcg tggcattgcc cataggacta taatgggatc
    22741 accgatacgt cacatttggt tttttaatcc gcccggcgca ctttttcaac gtggcgaaga
    22801 ccgttgccaa gcgaatgtgg aggcctcttc ggcggtttcg cttcgagcac ctaacgattt
    22861 ggcctacatg gcgtccgtta tgcgtgcggc aggcatccgt tgtgcgatta ccgattttcc
    22921 ggccgaaggc ggcacatggg atgattttga aaaacaattt tctgcgctca atccggatct
    22981 tgtcgtgatg agtatcacca acgctacgct gcgcgacgat atggaggcat ttcgccgaat
    23041 taaaaaaaat aatccttcgg taattactgt cgccaaaggc gcttggtttt gggcgggcga
    23101 tttgcgtggt ctgtctgaac ctgttttcga ggcgatggac tatgctcttg tcggtgaagc
    23161 ggaaacagaa ataaaaggat tggtcgaagg tttacacgcg catacggcgc ccggggatat
    23221 tcccaatatt atatttcgtt tgcctgatgg tacatggaag cgcacgcgca tcgaagcgtt
    23281 tgaaaccgat ctggatgcca taccgtttcc ggcaagagat ttgctaaaaa acgacctgta
    23341 cgtccgtccg gataccggtg aagcgcaggc tacgattcaa accgcacgag gttgcccgtc
    23401 gcagtgtgta ttctgtttga cgccggcaat ttccggtgca cgcgtccgtt cgcgttcccc
    23461 gcaaaatatc gtagatgaac tggaggaatg cgtcactcgc tatggtattc gcaatttttt
    23521 tttcaaagcc gatacgtttt cgatcaataa aaaatttgtt atcgcactgt gtcaagaaat
    23581 tcttcgtcgt aatctggaca ttgcctgggt ggccaatagc cgcgtagata ccgtggatga
    23641 ggaacgcttg gcgtggatga aaaaagccgg ttgttggctg gttgcattcg gttttgaaag
    23701 cggcaacgat gacgttttga aaaaaatgaa aaaagaagcc acggtcgaag atgcaagacg
    23761 tgccgcggct ttagttcggc aaggcggttt aaagatgtac ggttttttta tgatcggact
    23821 tccgtgggac aatgatcaaa ccatccgaga cacaatcgcg ttggcacggg aactggcttg
    23881 tgatttttat gaaatccact tagccacgcc ctttgaagga acggaattat atacgatcgc
    23941 tcaatccatg ggtttgctca cgacggcgcc gctcggtcac gactatttcg cggatccggc
    24001 gtccggtagt ctctttttat cacgggatca gttgatcgaa taccggaaat cgggtttaag
    24061 aagtctgtat ctgtcgccgt cctatattta taaaacagca cgatcagtca attcgccgaa
    24121 ggtattattg aattatgcgc gctacggtgt acgtcttttt agaaatttac tgaaggcctg
    24181 acgcatgcac gatcatattc aaaaaaaggt tcacaaattg cgtgatgaaa tcaatcgtca
    24241 cgattatttg tattacgtgc ttgggcagcc ggcgatcacg gatgaagcgt acgataaact
    24301 gatgcgcgaa ttaagtgaaa ttgaaacgcg gcatccggaa ttagtgaccc ctgattcgcc
    24361 gacgcaacgc gtgggttcgg atttgaccaa acattttccg acggtgcgtc atcaccaacc
    24421 gatgatgagc ctcggtaata cctacagccg tgaggaactt tccgattttg accggcgcgt
    24481 gcgggagggg cttgaagggg catcatttga gtatgtttgc gaactcaaat ttgacggcgt
    24541 tgctatgagc ctgatttacg aatacggtca cttcgtacga ggggttacgc gcggggacgg
    24601 agaaaaaggc gaggacgtca gtatcaatct caaaacgatt cgttcgatcc cgctgcgatt
    24661 ggatgcggcc aatgcccatc tggagcatat cgaagtgcgc ggggaagtat tgatgttgcg
    24721 cgaagatttc atcaaagtca acgcacggcg tacggaagca ggtgaaccgc cgtttgcgaa
    24781 tccgcgcaat ctgtcggcag gaacactcaa attgcaagat ccgcgtttgg tagcggcccg
    24841 ccctttgaag tttttttcat attacctgcg cgaacttggc gctgaaaaaa aattgaattc
    24901 gcattcggaa agtctgacgg cgttggaatc gctccatttt cccgtaatga aaacgtatcg
    24961 cgtgtgcaaa acattagatg aagtatttgc gttttgcgag gaatgggaag ccaaacgcga
    25021 caccttacct tttgaaattg acggcgcggt gatcaaggtt aatgcctttc gtcagcagga
    25081 aacgctgggt gcgacggcca aatccccgcg ttgggccatt gcgtataaat ttaaagcaca
    25141 aaaagtcgaa acccgcgtac gtgatattgt attgcaagtc ggacgcaccg gtacggttag
    25201 cccagttgcg gaattggagc ccgtgttttt ggccggttcc acgatcagcc gtgtgacttt
    25261 gcacaacgaa gattttttgc aggaaaaaga tattcgtatc ggcgataccg tgcgtatcga
    25321 aaaaggcggc gatgtgattc ccaaaatcac cgacgtggtt tttgataaac gccctgcgga
    25381 agcgcacgta tttcgttttc ccaaagcgtg tccggtttgt ggtgaacctg tcgtcaaaac
    25441 agaaggcgag gcggcgtggc gctgtgaaaa catcacctgc gatgcacaga tcaaaaagcg
    25501 tatcgaacat ttttgttcgc gcgatgcgat ggatattgaa aattgcggtg aagccgttgt
    25561 tgcgcaattg gtcgatgccg gatgtattca tgattttgcg gatttgtatt atcttcaaaa
    25621 agaggcgttg cttgcgttgg aacgttgggg tgaaaaaagc gctaccaatc tgatctcggc
    25681 tattgaacac agtaaacata attcactcga gcgtttgatc tacggcatag gcattcgttt
    25741 tgtcggcgaa gaaagcgcca agtatttagc caaacatttt aaatccctcg acgcactgcg
    25801 ccaagcggat ggtgagacac tcacggccat cgaaggtatc ggagaacgca cggcgctgag
    25861 tattaaacag ttttttgaca gcgcacaaaa ccgcaaagtg ctggacaagc tgacgcaagc
    25921 gggcgtacag acgatgtata tttcatctgc cgtagaattg cctccgatat ttagcggaaa
    25981 aacatttgta cttacgggga cgttgccttc acttggccgc aatgacgcta aaaaaatgat
    26041 cgaagatcgc ggtggaaaaa tatcgggttc cgtcagtaaa aaaacggatt atgttttggc
    26101 cggtgcggag gcgggttcca aattagataa agccaaggaa ctgaatgttc ctgtgataga
    26161 cgaaacagaa ttccttaaaa tgatcggcga cgccccatga aaagaacatt gacattaaaa
    26221 tggttatccg ttctgttaat ggggttggtc acggccccgc ttttgctatc ggtgcatgat
    26281 gcacgatcct acaaaatatc gccacgttta aaagctgttc ttgctgattc ggaaccggag
    26341 actaacgtgc acgtgtggat ttattttacg gataaaggtc aaacggcgtt gccaaaattc
    26401 aactctgaat ccttggggat atcgccggag gcggtaatgc gccgtcaaaa aatgggaacg
    26461 cgtcatgcga cggatgaagc cgattggccg gtagaaactt cctatatcga attacttcgt
    26521 caagccggtg tacaaattcg tcatacatcg cgttggctta atgccgtaag cgccgaagtg
    26581 ccggccgggc gtattcttga aattaccgaa tggtcattta tccaacggat tgaccgtatc
    26641 cagcgcatga aaagaacaaa acgtattgcc gaaacggaaa ataatcccat gccgttgccg
    26701 ctttccaaaa aatcatcttt ggactatggc gcttccctcg ctcaattgag tctgctaaaa
    26761 gttcctgaag tccatcaata cggttttgca ggtcaaggtg ttacgatcgc tgtttttgat
    26821 gccggttttg ataatctgag ccatgaagtt ttttccaaaa tgatcagaga agggcggttg
    26881 ctggccgata gtgattttgt caacggggat gacgatgtca gcgatgaagg tgatttgggc
    26941 gaagggagtc acggtacaca aacgctctcg acgatgggcg ggtataaacc cggccaattg
    27001 atcggaccgg cttataaggc ttcgttttta cttgctaaaa cagaaaatac cgaaagcgaa
    27061 acgcccgcag aagaagataa ttggattgcc gctatcgaat gggccgagcg tcggggcgcg
    27121 gatgtcatca gcagttcgct ggggtatatt cagtttgacg cgacatgttg caatcattat
    27181 gattctacgg attacaactg gcaatggatg accggcgatt cgacgcgcat tacgcaggcg
    27241 gccaatatgg ctgtggcccg tggggtcatc gtgatcaatt ctgcaggtaa tgaaggcgat
    27301 gcgacggtcg tgattaaaaa tacactgggt gcgccatcgg acggagatag tgtgatcgcc
    27361 gtcggtgcgg ttaatacttc cggcgtacgt acttcgttta gttcggtagg gcctactacg
    27421 cacggtgcga tcaaacccaa tgtcatggcg acgggatcga atgttcgtgc agcgggttcg
    27481 acgacaaata caagttatgt cggtgtttct ggaacgtcct tttcatgccc gctaacggcc
    27541 ggtatttgtg cgatgctatt gtccgcatat ccggatctaa ctcctgcaca agtgcggtat
    27601 gctttgcaag ctacggcaga tcgcgctgac aatccggata accattacgg ctacggtgtc
    27661 gttaatgcct gggatgcaat taattatttt ggtcaaatcg atagcggcgg catacttccg
    27721 gacaaattta aactccatca aaattcacct aatcctttca atccgcaaac aacgattcgg
    27781 tacgatgtac ttagggatgg gtatgttgcc atcatcgtgt ataacatgct gggccaacgt
    27841 gtacgaacgc tgtacgatgg atttcgttcg caacgacgaa actatgaagt gcaatgggat
    27901 ggtcgtgacg aatccggaca tcgcgtagcg tcgggagtat atttatacag gcttaccgcc
    27961 gacgggttta gtcaaaccaa acgcatgtta ctggtgaaat aaaaatactt agtgaaaaaa
    28021 acagactaat caataaattt cacctgtttg ttaaaacgga ttttcaaacc ttggacattt
    28081 cgtcggagta acgcatgaat attgccgtag caatgctggt ttcactcggt gcattatggt
    28141 tggcttaccg tttttacggt agccgtgttg cacagtggtt gggtgtagat gtatctcggc
    28201 cgacgcccgc gcagcgggta aatgacggtg ttgattttgt tccgaccaaa cccgtcgtat
    28261 tactcgggca tcattttgca tccattgctg cggccgggcc gatcgtaggt ccgacgatgg
    28321 ctttgttgta cggctatatg ccgacgtggt tgtggattat tttaggtgtg acgtttatcg
    28381 gcgcggtgca tgatttttca gcgttgttcg tcagcgttcg tgaagacgga cgatccatcg
    28441 cggagattgc caaacgtact cttggaaaaa ccgggttttt gttttttgta gcgtttgcca
    28501 ttttactctg cattctcgtc tgcgcggctt ttttacaatt gaccgccgta tccctcacgt
    28561 cgctgtaccc tttaaaagaa tttggcttgc cggaagatca gaatatttta aaaacggtta
    28621 cggataatgg cgttttaaaa ggtgtcatcg gcggcatcgc atcaacttcg gtgattgtta
    28681 tgaccttagt tgcgccattg atcggttatc ttctctataa aaaagaatcc ggtattttaa
    28741 cggtcagctt actggctttt attttttgcc ttgtttcggt gtgggtagga tttgtttatc
    28801 ccgtgacact tgatcctaaa atttggatga tggtgatctg cgcttattgt tttatcgcag
    28861 gttggattcc ggtttggctt gttttgcaac cacgggattt taccaatgta cagttgttat
    28921 acttcggtat tgtcgtaatg gttgccggta ttatcggaag cggcttggcc ggtgtagtga
    28981 tcgatgcgcc ggcgttcaat ttttccgcag aatcgtttca ggcgctcggt tatatgtggc
    29041 cgtttctttt tgtgacaatt gcctgcggtt cggtatccgg agcgcatgcc ctgatcgcca
    29101 ccggtacgac atccaaacaa ataggcagcg aaaaacatgc gccgatgatc ggttacggtg
    29161 cgatgcttct ggaagcgttg ctgggcattt gcgtgacgct catcatattg ggcgggcttg
    29221 gttatacgga ttatcacgac atcgtctggc cgcatgacga atttggaaat cctaaagccg
    29281 gtaatgcgcc attagcgttt gccgtcggcg tagggcatac tttagacaaa gggcttggta
    29341 tttcggtggt gtacggtacg gtgtttggta ttattctgct cgagggattt ctggtcacaa
    29401 cgatagatac catcgtgcgt ctgacacgtt atctttttga agaattgtgg ctcacgttga
    29461 tggaaaatcc ccatccgata tttcgtaata aattttttaa tacgttggta cccgtgatcg
    29521 gcatgatcgc gcttggtttt accaatgcat acaccgcgat ttggccgatt tttggttcag
    29581 ccaatcaact gctcgcggcg cttacactga cggctattac cgcatggctt atacagaaat
    29641 cgcgcccatt ttggtttacg gcgataccgg cgatttttat gtgtcttacg accattacat
    29701 cgctttcgct tttatttaaa cgctatttgg atgcacgcaa ctgggcgctg acgatcgccg
    29761 atgcgatact gttgctgctc acggtgggtg tcgtaataat gactttccgt tatttttata
    29821 atttacgtaa ggaactcagt tccaaaaaat ccgtatgaga cgggatatga aaaactattt
    29881 actgctttat atcattcttg tgttcgccgg cgttgtatca gcacaaacag gccttgcccg
    29941 gcgtcaggcg gatcagctct acgatcgggg cgataatttt cacgctatgg aggcgtacaa
    30001 aaatttgctc aatgataatc ccaatgatcc ggagttgaat tacaatgccg gcaacgcgct
    30061 gtaccgtttg gagcagtatg acgaagcggt caaattttat cagcgcgcat tggaaaacac
    30121 gagcgatgcc gatctcaaaa ataagatcgc atataacatg gcgaatattc aatacaagca
    30181 gaaaaagctt aaagaaagta tcgagggcta caaaaatgtt ctgcgtaaaa atcctgatga
    30241 cgccgacgca cgaaaaaact tagaatacgc gttgaaacaa atgcagatgc agcaaccgcc
    30301 gcctcaaaaa aatcaggata agaatcaatc caagcaggat aaaaaagaac aggatcaaaa
    30361 aaatcaggac gatcaaaatc aaccgcagga tgaaaacaaa gaagaacagc aaaacgacga
    30421 acagcaaaat aagaagaagt ccgatccgaa taaatccggt ttgaccaaaa aagaggctga
    30481 aaaattactg gatgcgctca aaaatcagga aaaagattat caaaaaaaga aaataaaaga
    30541 taaagccaag tcggattccg agacggaaaa agactggtaa ttttgtatat attaatgaac
    30601 aacgaggcct tttgttttat acttcgatac agttaacttt tgcctggagt gttttttttc
    30661 tcatcgtgat tgtcggactt tccgtcggat tatccgcttg gctctaccgt gaaaccattc
    30721 ctcccgtgtc tcaagttcgt cgccggatac ttataagttt gcgcagcgcg gcttttgcca
    30781 ttgctttttt tattttattt gaacccatac ttgatttgca gtacgaagaa aaagaaaaac
    30841 cggtagttgc ggtattgatt gatgcgtccg aaagcatgag cattcgcgat gcgcaaggtg
    30901 atcgccaaca gcaacggcgc ggcgtactcg atcatcgtat gtgggcggat catgccgatg
    30961 cgtgggatat cagatatttt acctttagtg atcgtttata tccgtccgat cataagccat
    31021 ccgatacact agccttgaca ggccatgcta ccgatatgcg taccgcgcta gagcaaaccc
    31081 aatcgctgtt tataggcaaa cacctggccg cgtatgtaat gattacggac ggtcaataca
    31141 atatcggcgg cgatcctggc ggatttgctc aagtatccga tgtaccggtt tttacgcttg
    31201 gtatcggcga tccagcggaa tctcgcgacg tagccattgt tcaaatttca cacaatgaaa
    31261 ccgcttatct gggagcgacg atcccggtaa ccgtgacgct ttcatcgtac ggctatcagg
    31321 gaaaacgtat tgatgtcgaa attttgcaca acggaaagcg cgtgcaaaac aaaatcgttg
    31381 tgttaccgga agacggagcg ctgacgcagg tgacgatgga tgtcataccc gatgaaattg
    31441 gtgatcagaa atatacggtg cgcgcaagtc ctttgctcgg cgaattatcc gatcggaaca
    31501 atcaacgcgc cttcgtcata cgtgttctca aaaacaagac atccgttaca ctgatctccg
    31561 gagcgcccgg tccggatcat cgttttattt acagcgtcct tatcgaaaat cccaatttgg
    31621 acgtacgtgc gtacgtcgaa caaaaaaacg ggacgctaac cgagttggaa caaccgcccg
    31681 ggcgaacgtc accgacggaa gcggatgttt atatttttaa cgactatccg acgaatacgt
    31741 ctgatccttc gacgttacaa cgctttctgc aggacatatc cgataacaat cgcagtgctg
    31801 ccatattctt cggaaaccat atcgaagtcg ggcgattaca atccattcag tcgcgacttc
    31861 ccatcaaagc taaaccggac gcgagtttgc aggaatcctc cgtctttatc ggtatgagcg
    31921 gatcggccag ccagagcacg atcgtggccg tttccgacga tccgggagag accatgcgac
    31981 agtgggccga tttgccaccg gtttggatca gccgttatgc tgtgacggca tctgaagagt
    32041 cggatgttct tgcgcgggta gatatgtcgc gaaccacgaa taccttgcgt ttacgaaacg
    32101 atattccgct gctgataacc aaacgcttgc ggcagcaaaa aacattagcc tttacgggct
    32161 atggattctg gaaagcggat tttattataa aaggtttaaa taaatcgaac gcggtatatc
    32221 ctgcgtttat tcaacaaagc gtacagtggc ttgcggccaa agatgatacc aaaccggtca
    32281 tcgtgcgcgc aggcaaatcc gtatatcgca gcggtgaagc ggtgatgctc acaacgcagg
    32341 tatatgatga acagtttcgc ccgataagcg atgcgaatgt gaatgtgact ttgcagaacg
    32401 gcagcgcgag ccatatgcat gcgatgcagc cgctcggaaa cggacgatac gaaataacgt
    32461 tggacggttt aatgccgggg gattatccta tcacttcttc ggcggaacgt tttggtgcat
    32521 tgattggcac ggataaaacc gctgtcaccg ttgaagcatt tagcctggaa atgagttcga
    32581 cagcgatgaa tgcgcggaca ctgcagacca tcgcatcggc ctccggcggg cgtttttttg
    32641 cctcatccgg tatagattca cttgccggtg tgatgaattt tcaaccgttg attttcgaac
    32701 gcgaaaaaaa tattcccgtc tggaatgcca cggcgctttt gattttgatc atccttttgc
    32761 tcagcgctga gtggtggatt cgcaaacgca gcgatatgtt atgagggttc tttcaatttg
    32821 atccgtgcat ccacgatttt gcaatcttcg gatttttcaa ataccatcag cggattgaca
    32881 tccatttcag tgatcgcttc cacgtccgtg accaattgcg aaagccgttg cagatattcg
    32941 acgacggttg cgatgtccgc cggtttttca ccgcggatac cttgcaataa cggataactg
    33001 cgcaaggact cgatcatctc ccgcgcatcg atatctgtca atggtgcgat tttaaacgcg
    33061 acatctttca tcacctcgac gtagatacct ccgaggccgc acatgagcac cgggccaaac
    33121 tgaggatcgc ggctcatacc aagtacgatt tctttaccgc ctttaaccat ttcttgtacc
    33181 agcatgcctt cgattttgtc ttgcagatgg tgcgccgtaa gatttttgat gatcgtctga
    33241 taaccgcgct gtacatcggc ttcggtacgc aggtctatga taacgccgcc tacgtccgtt
    33301 ttgtgtacga tctgggacga atgcactttg agtacgacgg gcttattgag ttttgcggca
    33361 aaggctacgg cttcggctcc cgttttgacg atcgtactgg aggccgtgcg caatccgtat
    33421 gcacggagaa taccgaatac ttcgtcttgc gaaagatacg ttcttcctgc ggcataacaa
    33481 tcgcgaatga ttttttcgac tgcggcttta tcaacttcaa atttgcgcag cgtaccttcc
    33541 tgcttaagcc gccatttacg atgttcattc atcgccgcta acgccaacgc cgccgattca
    33601 gggaatgtat aagtaggaat atcgtgtgta cgcatatagc tgatgtgaat tttttcctgg
    33661 ctcatacaac aactgagcac ggttttatcg gggaaacgtt tattgatcgc gacgatttgt
    33721 tcggccacgt cgtgcagaaa cactgtgatc ggtggaacaa aaatggccag tacgctgtcc
    33781 acattaggat ctttgcagat aatatcaagc gctttgccgt agatctgcgc gtcaccgccg
    33841 gccgtcgtat ccaccggatt attgacactg gcttccggtg aaagaatctg acgtagcgcg
    33901 gttttggttt gttgtgtaaa ctcggccaat tttaatccgt tgcttacgag cgcatccgtg
    33961 gccatgatcg ccggtccgcc ggcgttactc aaaatcgcaa cgtgatcacc gtgcggcagc
    34021 ggctgcttgt caaaagccat tgctacatca aaaagctgtt cgatggagga tacgcgcatc
    34081 acgcccgatt ggcgaaacat cgcgtccacg atcgtgtcgg aaccggacaa ggcgccggta
    34141 tgagacgatg cggcacgcgc tcccgccatg gtacgtcccg atttgacggc caatatcggt
    34201 ttgaatcgcg taatactgcg cgctacttcg ataaagcgcc ggggattgcc aaaactttca
    34261 aggtacatca atatcagttt agtgcgcgaa tcatcacgcc agtattccaa aagatcattg
    34321 ccggagatat ccggtttatt accgatgctg gcaaaattgg aaatgcccag ttcaagcgat
    34381 tcggcgtttt cgagaatggc tacgccgagc gcgccgcttt gggaaatgaa tgcgatattg
    34441 ccggagcgcg actgggtcgg cgaaaacgta gcattgagat tgacgtgttc atcggtattg
    34501 atcacgccca tacagttagg gccgaccata cgcatatcgt ttttttttat tttggccatg
    34561 agttgatctt cgagtgccgc accttccgca ccgacttctt tgaatcccgc cgtgatgacg
    34621 atcagacctt tgattccttt ggccgcgcat tcgtccacga cgccgagtac cagttgtttg
    34681 ggcacggtga tgatggctag atccacttcg tccggaatat ctttgacggt cggataagct
    34741 ttgatactat gtatggccgg tgcattcgga ttgacgggat atatcgcgcc ggtgaaaccg
    34801 cattcgatga ggttatgaat gatttcatgg ccgatggttt tgcttttgcg tgaagcgccg
    34861 atcacggcga cgttgcgggg tttgaatata gcgtcaagag cgtgcacgtt tttttccttt
    34921 gcgggttgta gttttatcag taatttgaac cgcaaaaaat atatgcattc cggcggcaaa
    34981 atacatgagc attgtcatgt ttacttttat gcgataaaat tttcaataaa gttaaagttc
    35041 caaagcatca aacataacga cattatatat taattaattt tattcccatc atgccaaaat
    35101 atcctcatac agccgaacgc gtgaaagagc ttaccggatc ggtgtacgaa aagtaccgtc
    35161 atttgatcaa acagcagaca tcggttgcta aattgcatat cggagatacg gcgttatggc
    35221 cgccgtatgc gatgcccttg gccgactatt tcacatcggc acaccgtgat atgaccaaat
    35281 attgcaacac gtacggcgtg gatgagctgc gtaaaagcat tgccgaaaaa gtaagcgaag
    35341 acaacggtat cacttccact cgggatcatg ttttagtgac ggccggagcg agcaatgcgt
    35401 tgagtgcggc gatgatgact ctgatcaatc ccggcgacga agtgatcgtg ttcgcgccgt
    35461 gctggccatt ctttacggga atggtgcgtg ccgcgggcgg aaaagtggtc gaagttccgc
    35521 tgtacatgga cttatatgac caaccggaaa tggatatttt cggaaagctc aaaaatgcaa
    35581 cgacttccaa aacggtggcc gtgtatgtca attcgcccaa taacccgtca ggaaaaatgt
    35641 tggatgctcg acaatggcag actgtcgttg attttgtaaa acagcatcag ttgtggctga
    35701 tcagcgatga agcatacgac ggtttggcat ttgacggacg taaaacgatt tcccccgcta
    35761 cgttgggtat acgcgaacaa accatcagca tttttacgtt ttctaaaata tttatgttcg
    35821 cgggattgcg cctcggctac gcgatcgcgg agcctgaact tatccatgca ttgaataaag
    35881 tcatggtgca ccagttatat agcccttcgc cggtggggca gtatatgatg gtcgaaccgt
    35941 tgcggcgtcg gcacgagtgg atgcacggga tcaatgctca ataccaacaa ctgcgcgatg
    36001 cgatggtgga agcactggcc atgccggtcg ctaaaccgga gggaacgtat tttgtttttt
    36061 tagatgcagc gccgtatttg caaggtcggg catttgatca actggttgag caaatgatta
    36121 tgggcggcgt attggtcgcg ccgggcggtg attttggcaa agatttttct tcgtatatac
    36181 gcctctgctt tacgggtgaa ccggaatctg taatcacatc gtcggctcag gttattcgaa
    36241 atgtattatt gcaaaacgga caaacgtcat aaaaatctgt ataggaatga aaacatgaaa
    36301 atcggatacg tgcaattcaa accgcaattt ggcgatatag attataatgc tgccaaaatc
    36361 tacgcacttt tagaaaagaa gcaggctgat ctgatggtat tacctgaatt ggccacaacg
    36421 ggatattatt ttcgcgatcg taaggaagcg tatgcgttat ccgaagatcc gaaaaaatca
    36481 tttgtcgtgg aagtactggt cgagttatgc aaaaaaaacg gtatgcacgt gatcaccggg
    36541 ttcaatgaac ggaacggacg aaaaaattat aacagcgcct tgctaatcgg taaaaaaggc
    36601 attgtggatg tgtaccgtaa aattcattta tttaacactg aaaaaaattg tttcacaccg
    36661 ggcgataaac cgctgcgcgt tcataaaatc gcttcggcac gggtcggtat gatgatttgt
    36721 ttcgactgga tttttcccga agtgacgcgc acactgtcac tgatgggcgc ggacatcatc
    36781 gctcatcctt ccaatctggt tttgaactat tgccaaaatg ccatgattac ccgatccatc
    36841 gaaaatatgg tgtattgtgt cacagccaat cgttatggcg aagaaacacg tccccacggt
    36901 gatcttacct ttacaggaca aagtcagatc gttgccccca aaggcgtgtt acttcatcgt
    36961 gcgccggaac aaaaagaaga agcctttatc gcaaagattg atttgaccaa gtcacgtcaa
    37021 aaaaacatga cgccgaaaaa cgatcttttc aaagatcgaa gaacgaagtt ttatctctga
    37081 tgcctatatc attttctcat cttagtagag gttaatatga attatcgtat cctgctattg
    37141 tgggtttgtg tatccactac tgtatccgca caacaagtca attatgaagt tcgctttccc
    37201 aacattcgcc atcatgaagc ggaagtgacg gccgtgtttt cggatatttc gtcacccgtt
    37261 ttggatattc ggatgagccg ttcctcaccg gggcgttatg cactgcacga atttatcaaa
    37321 aatgtatatt cggttagagc tgaggacggg cgcggaaaaa aacttgatgt aacccaaaat
    37381 gatccttacg ggtggcaggt atccgctcat gacggaacgg tgcgtttttt ttatacagtg
    37441 tacggcgatc atatagatgg cacgtattta tcgctcgatg cgtcgcacgc gcatatcaat
    37501 cccccggcga tgtttgtgta tgcacaaaac gcagatcatc gcaatatccg ccttcggttt
    37561 acgcttccgg ataaaacatg gcgcatcgct acacaactca aagagtccgg tgcttcagta
    37621 tacattgctt cggatttggc ttatctgatg gatagtccta ttgagatcag cgcatttcag
    37681 gaatcgtcat ggcgcgttcc ggcgacaagt ggtgatattc ttttttcgct gatacttcat
    37741 catacgggaa cgccgaaaca gacagaatat ttggcaaaag tcggcgatgg gatcgtccgc
    37801 gaagcgcgtg cggtgtttgg tgaatttccg gtttttgatc agggacggta tgcctttatg
    37861 atggaattga cgccgtatgc gtcctgggac gggatggaac accgcaattc atgcgtgatc
    37921 accaatcggg attctatcaa cggtgatttt ttggatatcg catctacgat ggcgcatgaa
    37981 ttttttcacg cttggaatgt ggagcgtctt cgtcccaaat cgctagaacc gtttgataaa
    38041 gcgcgggtca atatgtcgga ggaactttgg tttgccgagg gttttacttc ctattatgat
    38101 gatttgatca ttacacgggc gggcattcaa agcgtggatc ggtattgcaa ggtattaacc
    38161 aaattgatga ataacgttgt cgtggatccc tccaaaaatt ttggcggtcc ggcacacatg
    38221 agccaacgtg cggcgtttac cgatgccgcg acttcggtgg atgcgcaaaa tcgtaaaaac
    38281 atattttcga cgtattactg gtacggcgct acggtggctt tgggattgga tctgacgttg
    38341 cgcgcggaac atggcaaaac gttggacgac tacatgcagc ttttgtggaa agcatacggt
    38401 aaaacggaaa aaccctatac ttcagccgat ctcgaatcgg cgttaggtga tctgatcggc
    38461 gataaaaaac gggcgcaacg tttttttgat gaaacgattc gcggtaaccg gttaccggat
    38521 tataaaaagc tgctggatta tgccggtttc gtcacacgtg ctgcacatgc ggatagaccg
    38581 acattaggac aagtgcggct tgcagccgat gacgacggta tcctcatcaa agatgccacg
    38641 ctcataggcg atccgctgta tctggccggt ctggatcgcg gcgataaaat tttttcgctc
    38701 aatgatcaag ctgtgcattc catcgcggcg tgggaagagg ttttgacaca attaaaaccc
    38761 gatgacagcg taacgattgt ttatgaacag cgcggcctca aaggtatatc gcgggtcaaa
    38821 gttgctgcag atccgtcttt ggaaattata ccggtcgagc aaaccggtaa gccgctgtca
    38881 gaaaaaaaat caaactttcg tgcagcatgg ctttcatcca aatctacaga caagccgttg
    38941 ttgtatcggt actgcaaaaa gtgtcggcgt gcgtacgaat ttcaatacga attctgcccg
    39001 tttgacggcg tgcttttaca aataacaccg gaggagtaaa tcgtgcatct ggaaaccatg
    39061 aaagcggcgg tattgcaggt ggtcggctct attgaaaatt tgcattattc cgacatctct
    39121 aaaccggaac ctaagccggg tgaagcgttg atccgaatta atgcagcggc tctgaatcat
    39181 cgtgatctct ggatcgtatt tggtaaatat gccaaaatac aagttccggc tgttttgggt
    39241 tccgatgcat gcggcacggt tgagcaagtc ggtgacatga aggataatat ttgggttggt
    39301 cgccgtgttg ttgttcatcc cggtttgttt tgggggaaca acccgaaggc acagggaaaa
    39361 aattacaaaa tcttgggcat gcccgatcag ggtacgcttg cggagtat
//
LOCUS       DAOQGF010000018        38086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_459_length_38086_cov_7.096763, whole genome shotgun sequence.
ACCESSION   DAOQGF010000018 DAOQGF010000000
VERSION     DAOQGF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 38086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_459_length_38086_cov_7.096763"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PK937_04425"
     CDS             complement(<1..1296)
                     /locus_tag="PK937_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31845.1"
                     /translation="MKKTYLYIFLFLFSVNLLFTGGRIASSDETAYFLEAQSVVERGT
                     LAIPTSIVNNGAFGVDGRFYVGGGVGYTILGAPMYAFGLLITKALPIPETYSLFVLKG
                     IFTLINPILCALLGLLMIHYLRFMSVSWGFAFLWASALLFTTNVFVYSKSSMREPLIT
                     VCVLMAIMEIFTFGRTSLRKHLHLAGIAAGWLVLIKPSFLIFVPVFGLYLVWVLYDNR
                     LHVKDWLRAANVVIPVSIFSAWVSVFIGMAVCYNWLIFGGPFTSGYSKIQNPFSNPFL
                     VGVYGLLFSSGKSLFLYAPLTILIFPAARCFLQREFKLFTAITVVCCTFIALHAKFVA
                     WAGDGSWGPRYLLPIVPLLFIVIGTYAYETIKKNRTLRFMIQTLAVIGLLIQFGGMTI
                     YLGSYYRYLGEYPYTREFSDPEFLYKSHFVPNYSPVVGHW"
     gene            complement(1293..2411)
                     /locus_tag="PK937_04430"
     CDS             complement(1293..2411)
                     /locus_tag="PK937_04430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH31846.1"
                     /translation="MKVVWFSEIKWTYLKTRKQQIIENFPPEWEVLFIESFVLGKNNQ
                     FQPVREGRVTYATVPFFKATPYPLINKLQVLWPFRTVIEWIASLWVFWLCRKTGFDAP
                     ERVIATSNIYYADIIRRMKKKAVIYDCNDYPLGFALLLPTAKEFFKKTIRLSNVTTTV
                     SKKLVEDIIPHNPSRVSVIGNGVDFKLFDSKPAIKPVEMPTDRPVVFFSGAVAEWFNV
                     ELLTRLIESLPEAAFVVLGPIKHAVTGEQLRILAQVYGLILISEKKHAELPSYMHSAS
                     VCILPFRNNRRIWGANPNTLYEFLACGKPTVTFGFSEEVRNLAPHVYIADSDEDFVQY
                     VGKALRSKADTEKLKSLAAKNSWSIKAAQMTDEIRRVV"
     gene            complement(2419..3357)
                     /gene="fmt"
                     /locus_tag="PK937_04435"
     CDS             complement(2419..3357)
                     /gene="fmt"
                     /locus_tag="PK937_04435"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNH31847.1"
                     /translation="MKKLRIVFMGTAAFAVPSLRRLAQSHHEVVAVVTTPDLPRGRGQ
                     QVSFTPIKELAVELGLPVLQPDNLKDEQFIASLRSYNADVFLVVAYRILPPEVFELPS
                     LGCINLHASLLPKYRGAAPINWAIMRGETETGVTTFFIQKKVDTGTIILQRKTIIGEN
                     ETVGELHDRLSEIGADVFLETADLIAENKVVTRVQDDQLATTAPKLFKETCRIDWTRS
                     SREIKNTVRGLSPYPCAWTTIDGEAYKIFRIETVKTAEQSAPGTIIVSNKQDGIWIQT
                     GDGVVAILELQPPSKKRMKVTDFLNGRQLPIGICAG"
     gene            3789..5225
                     /locus_tag="PK937_04440"
     CDS             3789..5225
                     /locus_tag="PK937_04440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNH31848.1"
                     /translation="MSLPLIIQGGMGVAISDWRLARAVSMTGQLGVVSGTGISRIFTS
                     RLMQGDVSGMMREALSHFPSKESVQSVLERYFISGGKSAEEPYKSQPVYSLNPSVFLE
                     QLTTLANYVEVFLAKREHTGIVGINLLEKVQMPNLASLYGAMLAGVDYVLMGAGIPTQ
                     IAGILDKLRNHEPVSYRLDVQNAAPNDDIRFHFDPRKIFPNMAKLTGALKRPKFIPIV
                     SSTVLAQALIKRSEGKIDGFIVETPTAGGHNAPPRGPMRLNERGEPIYGEKDKVDIAK
                     LKELGLPFWLAGGFGHPEKLKEAINNGAAGIQVGTLFSLCDESGMDAQLKKQTLQNVL
                     DKNSQIITNPVISPTGFPFKVTQLKGTVSDPEVYSARERICDLSLLRTVSKNDDGTIV
                     YRCPAEPVEEYVRKGGNVEDTNGRTCLCNNLVAAAGFPQVRKNGYVEPPLVTSGDDLG
                     SVKNFITKDKLHYSAKEVIHYLTEQNNH"
     gene            complement(5392..6192)
                     /locus_tag="PK937_04445"
     CDS             complement(5392..6192)
                     /locus_tag="PK937_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31849.1"
                     /translation="MSLNLLKNSTAFLYVTFFLVLIDVKAQQEDLLLLKAKDHAMQYY
                     VSLPKGWSNTKTWPVLVVCEAAEKQYKENALRFIKARGERPYIIVAPFIVTNGNQGLR
                     NPEIFPYTSDTWDQIDREGTCTFDLQGLDAVLKDVTQKYNGEARIHITGFEAGAHLAW
                     AMTFLQPEKIKSIVVVAGNFRSRCLGEAILPVAGEHRNLPLINLVGSLDSSWSSGGRL
                     YDQWLEGKTLAQRQGYVNITEHVIQDKGHRPMPEDVFIQIESLRQKSK"
     gene            complement(6300..8138)
                     /locus_tag="PK937_04450"
     CDS             complement(6300..8138)
                     /locus_tag="PK937_04450"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNH31850.1"
                     /translation="MKILSAIASIIITAGSVLAGDKLDLRYDVDLTKIEKDSFFVTLH
                     VKGIQNDTTVFQFASTAPGTYQVMDIGRFVGGFTAYDEKGRVLPVIHRSVNQYVIAGA
                     KKLHTIRYQVEDSYDTIIKEDQVGPMSGSSIETDNVLLNGQMVFGYFQGFQSNPMQIK
                     YNYPQGWLVGTALEEKNGTYKAKTFDEVVDSPFLFGKLTKAGMKIGGAQVDVFCHAKN
                     GTMAADSLLSHLKDILISAEQFLGGLPVKRYAFLYKFTSDPLPAWGAWEHSYSSFYIL
                     PESISFPSGGGMGSGLADIIRSTAAHEFFHIVTPLNIHSEIIERFNFEQPTPSRHLWF
                     YEGVTEWAAQIMQVRSGLVQPNEFCERVSQKMSVNDNYDPRVSLVDLSLGSYGHLNAQ
                     YQNIYAKGALTAMLLDMRLLELSKGKTGLRDVVLKLSKKYGPDKAFPDSEFFEINVKM
                     TYPEIDDFIKRYIQGNEPLPVSEYIAKAGYDYVKSKPTGRFRSVRGTFSLSLDHDKVV
                     VSGVDTTDEVNRQTGIREGDVILGMWYQDKLYDLRDPAMQQIRQTMQPGESFAWKIER
                     DTKEMMLQGTAGKIEIMENHVITPVSEPSAEQQQFHSWWLKPFKGK"
     gene            complement(8161..9960)
                     /locus_tag="PK937_04455"
     CDS             complement(8161..9960)
                     /locus_tag="PK937_04455"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNH31851.1"
                     /translation="MRWMKLWVMAVTFSGSLIAGDIKLNYRVDISEPMQDRFYVTLVV
                     KGWSNDSAVFQFASTAPGTYQVMDVGRFVNGFTASDSKGKSLPVVQRGINQYVINDAK
                     NLATISYRVEDVNDTNLAVHPIYPMAGSNIEKDNALINGQMVFGYFHGYQANPIQVQI
                     DCPKDWKVATALTPKNGLYTADSFDHIVDSPFLLGRLTEVKTKVGGAEVGIYCYSQNN
                     IVTADSLLEALQAMLVAAEKFLGELPVKRYAFLYHFRQDVPAAYGAWEHSYSSEYVMP
                     EKPMSVYGETVKDFSAHEFFHIVTPLNIHSEIIQQFNFVKPTPSDHLWLYEATTEWAS
                     HLMQVRGGMITSDEFKERITNKLVTSDYFRSDLSLVDLARGSFGDLAPQYGNIYIRGA
                     LTMMLFDMRLLELTKGKMGLNDLVKKLAKQYGPKKAFPEKEIFDIVVKMTHPDMKDFI
                     DRYIKGYEPLPVESMLNLAGYAYHAKFETNEEKTDMGKFDFDYGTDKITFKDVDASNA
                     VTAKLGLQNGDAFLGFIKDGTEFRLGKPGVQKAMNSVKPGDEFGLIVQRGSEEVKLTA
                     KAGVKKNIKPHVILPVEKPTSDQLAFRKRWLSN"
     gene            complement(10059..11867)
                     /locus_tag="PK937_04460"
     CDS             complement(10059..11867)
                     /locus_tag="PK937_04460"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31852.1"
                     /translation="MTRRAFFLWIIFAHKAVSAFAQLPELTYRMDLTSLDDDSFFVQL
                     DIKKYPHSSALFQFPMTAPGTYAILDAGRFVGSFSALDTSGHELTVTRTSVNQFRIDE
                     ARRLARIVYKVNDSFGERIGENPIHPMGGTNIEPDNIVINGFMTFGYFSEFQNIPYRL
                     RVTMPADWKLGTALVSKDGYLIAPTYDDLVDAPLMAGRLTTAHWTMDKTKISVYCYSE
                     KNRVNATWLRSQLKDILNASKKFIGTLPVDRYVFLFHFREDTGPIYGALEHNYSSFYV
                     IPEMKLEGLRPIVQSIAAHEFFHIITPLNLHSEKIVPYRFDRPTPSRHLWLYEGVTEW
                     ASDIMQVRAGVIPDSVYMQRLREKLQRNDRYKPDISLETMSLGAYDVYEDQYANIYER
                     GALTAALLDMKLLELSDGKRGLRDVIMALVKKYGAGKSFTDSSLYGIIVDHTYPEVRE
                     FMDRYIRGSERLPIAEYLIKAGYQYESKYETTRMRNHLGLFEVMQKDTMVIVTSVDPN
                     DSVCTRLGLQAGDIIEKMVLNNIDLPLTDVSLIEFFSFIDEGASFAWVVRRGSQTLTL
                     NALAGRAPVIEKHRIRPISDPTPEQLRFRRIWLHIP"
     gene            complement(11864..12190)
                     /locus_tag="PK937_04465"
     CDS             complement(11864..12190)
                     /locus_tag="PK937_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNH31853.1"
                     /translation="MISYDDFDKVEIRTGTIIEVNDFHKARKPAYQLTIDFGVAGIKR
                     SSAQITDMYDKQALIGKQILAVVNFPPKQIANFFSECLVLGVVTPEGVVLLQTERPVA
                     NGLRIS"
     gene            complement(12187..12945)
                     /locus_tag="PK937_04470"
     CDS             complement(12187..12945)
                     /locus_tag="PK937_04470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNH31854.1"
                     /translation="MSTRLGIDVGNTHIKIGVLDGERVVDVWRFRTDSERSVDEYYFQ
                     LQNVCREAKLDTIPELLIASVVPSVTIAFQLLEETRGVKSSFIDVKSPLSYGFHPAIN
                     HQVGADILILAEAAVRLKGKDVIIVSAGTATTVFAVSDGVFQGGAISPGIKGSVESLI
                     AKAALLKTVYLDVPNSPIGQNTDDAVRSGVIFGYAGMIDGLVTRMREQLGKPDMPVLA
                     SGGMIAKFAKVSRTIHEIKPDLGIQGLAYLSGKL"
     gene            complement(12942..13499)
                     /locus_tag="PK937_04475"
     CDS             complement(12942..13499)
                     /locus_tag="PK937_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNH31855.1"
                     /translation="MKIHVIAYDAQWAVLFESESRRLTKFWPDKTLSIEHIGSTAVPG
                     LAAKPVVDILIGTDDMIELQAMVSKLTDADYRYIPEYEKYIPERRFFIRPKNNVEEKS
                     INWNPDYGARDHLFHIHAAVRTSPFWGRHITFRDVLRRDKALRDAYADLKNKLSENVP
                     EDKNTYAQGKFEFIQAVGKKLGVQV"
     gene            13602..14030
                     /gene="aroQ"
                     /locus_tag="PK937_04480"
     CDS             13602..14030
                     /gene="aroQ"
                     /locus_tag="PK937_04480"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010274081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNH31856.1"
                     /translation="MKFLILNGPNLNLLGEREPHIYGTQTLADIEDNLKTVFPEHTLE
                     FKQSNMEGELIDLLHAVRKTHNGVVFNPGAYSHYSIALQDAITSIQIPVVEVHLSNIH
                     AREEFRSRSVVARACIGQITGFGSYGYEMAIRTLIQHHTK"
     gene            complement(14059..14523)
                     /locus_tag="PK937_04485"
     CDS             complement(14059..14523)
                     /locus_tag="PK937_04485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH31857.1"
                     /translation="MEAVAPKPKYKTFTYHTGLHRIATRAAMLSSEDKPAFRVASPPE
                     FKGETDVWTPEDLFVAAAEICTMTTFISFTEKAGLHIADYQSRANGILEFVEGGYRFT
                     KIIVRPKIAVDNEADLEKARSVMHDAHKKCLIANSMKTEVIVEAEFVITEAA"
     gene            complement(14664..15707)
                     /gene="sucD"
                     /locus_tag="PK937_04490"
     CDS             complement(14664..15707)
                     /gene="sucD"
                     /locus_tag="PK937_04490"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNH31858.1"
                     /translation="MSVLVDKKTRLVVQGITGKEGTFHTSQMTEYGPYVVAGVTPGKG
                     GLVYKGNEKDKLKYGVPVFNSVKDAVEKAKANTSVIFVPPAFAADAIIEAAEAGIKVI
                     ICITEGIPAADMVRAYNFIKDKGVHLVGPNCPGVISPGKCKVGIMPAFIHKPGRVGVI
                     SRSGTLTYEAVGQLTALKIGQSTCIGIGGDPVIGTRFVDAFKLFAEDKDTDAIIMIGE
                     IGGTAEEEAAAWVKKNFKKPVVAFIAGQTAPPGRRMGHAGAIISGGKGTAEEKMKALK
                     SAGIHVVKSPADLGVTVAQVLGIKVKSTTSAKPAVKVEAKKMDKKKDKKSDKKSKKSD
                     KKKDKKKDKKKKK"
     gene            complement(15733..16191)
                     /locus_tag="PK937_04495"
     CDS             complement(15733..16191)
                     /locus_tag="PK937_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNH31859.1"
                     /translation="MLNAHKLLGTALGVGYLPKAPGTWGSLLALAIYFIFFPSSSSLL
                     IHAVFFIIIAAVFGLGVVSATRLEKVYGHDPSCVVIDEVVGLWIALWFLPKTWIVMIG
                     AFVLFRFFDITKWFGINRLQDLPKGWGVMMDDVLAGIWANVILQIVVRIY"
     gene            complement(16197..16823)
                     /gene="pgsA"
                     /locus_tag="PK937_04500"
     CDS             complement(16197..16823)
                     /gene="pgsA"
                     /locus_tag="PK937_04500"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNH31860.1"
                     /translation="MFILAMRFIPNILTILRILLTPVFVFCLLSDEYQVYSVALFVFA
                     SVTDWVDGYLARKYSYHSGLGKFLDPLADKILVLSAFFSFVYLGFVKLWMVMVIVIRD
                     FMITSLRSYAIRRGKPMMTNFFAKLKTAFQMLMIGLILIFIAMQSLIHMTISEQLNYF
                     IYIVTHYEVIYVGMLVVTAVTVISGLLYLYENRESLRSLHSPSQVKNT"
     gene            16896..17678
                     /locus_tag="PK937_04505"
     CDS             16896..17678
                     /locus_tag="PK937_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNH31861.1"
                     /translation="MRKTWQWIDTGKQPGSFNMAFDEYLAAEVGSGLRPTTLRFFQWQ
                     PYCISLGKHQNISEINSEKAHHDGLEIVFRPTGGRAILHAQELTYSIVYHDDIRGSVE
                     ESHAHISTAIAIALRRLGYEAYCTTEQTDFKQHYRNPSSALCFSSSAKSEIQIEGKKV
                     VGSAQRRYAQATLQHGSILIGDFHRKLADYIHADAATKNILQRVMNEKTTELNQYIAV
                     DIAALKESLREAFSEFFSIDWTDGAWQADAIASHHQRSAFMV"
     gene            complement(17646..18410)
                     /locus_tag="PK937_04510"
     CDS             complement(17646..18410)
                     /locus_tag="PK937_04510"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNH31862.1"
                     /translation="MSFRDIVVLFEDEHFIAVHKPSGLLTIADRFDTKAPNLADLLRD
                     ERGFVFKVHRLDKPTSGVVLFAKHKEAHQSVSLQFEKHTIQKNYLALVSGRVQEKQYN
                     VDVPIGEAERGLMKIDRTHGKPSETEFSVIESFTRWTWLEARPKTGRTHQIRIHCLAK
                     GTPIVGDMHYGSETGIFLSQLKKNYKRTGDSDEKALPGRLALHAAALTFLHPWENRPI
                     TVSASLPKDLQSTLNQLRKYAAVAASSHHESGALMM"
     gene            complement(18465..19817)
                     /locus_tag="PK937_04515"
     CDS             complement(18465..19817)
                     /locus_tag="PK937_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31863.1"
                     /translation="MSQRAKQWTYHLSVAVLAIGMVVVFNFLVCHPVDFVRTNTKPAE
                     WKTYANTKFGFSVEIPSTWSIREDAQGVELYDAETRRYISVKALAEESSLLNIPTDEY
                     TIERSVLRHTYVCDTIREPHRVVTKSGLTGYMIAPPLRNDVDRSQINAPWADRCDAST
                     LSLTYFPVQFAGDIYASFVEFLSTGPSDAVYEHMTQSYRHLFKTLRQGELMRMHLIDT
                     SETPSPAVAYVGETRGFYVDFDADGTDELLIAGMPRNMEDTKERCFFRVLKKQGDQWV
                     WAFERKYSENSFHSSDIKIVNLDNRFGPDVFLRFVEHGNPWGKNSTVIIFWDGDKFRG
                     ADFGAFGDARDLNGDGTDEVIHTTNSYFSQGAIASWYDVYTYDKGEFIEVNSRFPEFY
                     KNTVLPKYRDQYETVQGELALTRVEQFKGPARQLMFRLSRYIDMSTDISEGRPARERW
                     "
     gene            complement(19849..20391)
                     /locus_tag="PK937_04520"
     CDS             complement(19849..20391)
                     /locus_tag="PK937_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31864.1"
                     /translation="MMEFISAQWFLWRSRFLKPTEVMDFPQRLSEIKRVLVYFPMAID
                     KSRAETIFGQIQKTLSGCTLDFLLHPETPQEVSAFLTERQATVTILAKTDFGLLRTLN
                     GEAVDRIRAKEYDMILDFTFTADWAMAWALRACGVSVLAGAFTEPRSERFHNLLIKAH
                     DAPTFDATLFGCLAQMKTVI"
     gene            complement(20411..21721)
                     /locus_tag="PK937_04525"
     CDS             complement(20411..21721)
                     /locus_tag="PK937_04525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH31865.1"
                     /translation="MIISKFFFRRFFWGAVAVSVILTGSLSAQTGRQLIDKIAAVVDD
                     EAIMMSEVQQYAYFEAMNLHINPSNTEEFDKIMKRVLETMIQQKIMLAQARFDSITID
                     EAQVEQSLEQQIRERVQQAGGEDELERAMGKSVKELKRLYRPNIRKQMLAQRIQSTKF
                     NDVKVSRSEIEKFFEEFQDSLPQIGASVKFSQILLEVKAGDEAFAEARRMAQMYLDSI
                     KKGADFATIAKAVSTDPGSGKKGGDLGWFNRNDFVKEFADAAVRLEPGEISDVVKSEF
                     GYHIIQMIEKSGERIHVRHILLGVANGPEDKTRTREKLGRIRQDILEGKISFEKAALQ
                     YSDDPAKKGNLGNMGWIEIATLQDKSFVKALDPLKNEEISEPFETPFGFFIVQLHDRR
                     DTRKLNLNEDWQTIENFALRQKQAKEMDKWLEKIKSKFFIDVRL"
     gene            complement(21764..22156)
                     /locus_tag="PK937_04530"
     CDS             complement(21764..22156)
                     /locus_tag="PK937_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31866.1"
                     /translation="MGGSQLKKEFLYISVLMWSFVVSGWAQKETPKAAPKSYVAAVFT
                     IGKNWDTTKTAYEQPHFKEHGANLKRLHAEGKIALGARYGEFGLVVFKTENMEEVRAC
                     FDQDSIVIKDILRMDIQPFRPFYKGCID"
     gene            complement(22138..23613)
                     /locus_tag="PK937_04535"
     CDS             complement(22138..23613)
                     /locus_tag="PK937_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH31867.1"
                     /translation="MPAPADVKIFDVKNTKLFINNTFVDAASNRTFETINPATGKAIC
                     AISEGDKNDIDTAVAAARKAFSDGSKWSRMSGSRRGKLLWKISELITKNADELAYLET
                     VDCGKPISETKNIEIPVTADIFAYYAGAATKIEGETIPVNGNYFNYTLREPLGVLGLI
                     IPWNFPLITAARKIAAALAAGNTVVVKPSEFTPLTTLKLAELFAEAGFPEGVFNVVTG
                     FGKTAGAALVAHADVDGIAFTGSTTTGQEIMKTASLTMKRLSLELGGKSPNIVFADTD
                     LDTTIKMAAAAIFYNKGEVCTAGSRLLIEESIHDEFIEKLAARAAKMMPGDPLNPETK
                     LGPLTSELQMNKVMSYVQAGKSEGAALLAGGNRIGNDGYFFQPTVFGNVDTSMKIATE
                     EIFGPVLATMKFKDFDDVVEKANRTYYGLAAGIWTRDIKKAHTLAKKIKAGTVWINTY
                     NMYDAAMPYGGYKMSGFTRECGMEAIYEFYTQVKSVWVDLN"
     gene            complement(23916..25184)
                     /locus_tag="PK937_04540"
     CDS             complement(23916..25184)
                     /locus_tag="PK937_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH31868.1"
                     /translation="MKKLVMLLMLGVGFNVYAGDKIPGFVYGDVKSVLNAAREKKQVV
                     MIDFYTAWCGPCKLLEKNVYRSAAFLPVTEKMVCYKIDAEKGEGIELAKKYNITGYPS
                     VVFMYADGKEIERKVGYNEDIEGYIADLMRIAEAKDVLPVWMAEYNANKNLTLGEKIV
                     RYYLSVDPRSAEPFYTELMAMPGMSDEQSAALKQQHAYRGLDYGLESAAKEADEIIRT
                     SKVEDDYDLISLAFNYAFYLTRRNQKEKAWEIASALYAKLDAEKRQSYAENFMEIKYR
                     SGQTDKSEDETRLRGIDLSDANGVSEAVKIHIKWKEKERAENVLKTWLSSYGTNASLD
                     DMNNVGWTAFEVKIMMREFAAAMIKTWDETVLEYQDAYKADTIANLCEAVGDKANAVR
                     FGQLAVNMIPERSRMRKEFEDNLKRYQAMP"
     gene            complement(25265..26482)
                     /locus_tag="PK937_04545"
     CDS             complement(25265..26482)
                     /locus_tag="PK937_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31869.1"
                     /translation="MNILESLRSALWALTSNRLRTFLTMLGIIIGVGAVITMVAIGEG
                     SRRRIQERLQGLGTNQLLVRPGSQNVGRTVLAAGSSQTLTEEDAETILKTSEHVSAVS
                     PELSRNAQVKYESKNTNTTITGTRIEFLKVRNFVLAEGRYFSAEEIKGNVKVAVLGQT
                     VEETLFGSAQSVGSIIKINGQNFTVIGVLAGKGQTGMGQNQDDQIMVPLTTAQTRLFG
                     LDFVTTIAVQVYDENMLDETTFDIERALRKTHKLRGDAENDFSIRNQADILATAQETS
                     DTMAILLASIAAISLIVGGIGIMNIMVVSVTERTKEIGIRKAIGAKRKDILLQFLIEA
                     MVICLFGGLIGVGIGIGSAYVLQWNAGWRTVVTPDSVLLSFGLSVLVGIFFGFYPALK
                     ASQANVIDALRYE"
     gene            complement(26522..27802)
                     /locus_tag="PK937_04550"
     CDS             complement(26522..27802)
                     /locus_tag="PK937_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH31870.1"
                     /translation="MKKYILGSIVLCLCVGALYLWKGNGSDQTKRNASGSALVIRPAA
                     LERGRLNVDVSATGVINPINVVEVKSKASGLVEKIPIEASDVVHSGQLIARLDQTDTR
                     NALEQAIADSQVASAVLIQQENNWRRAKDLFEKNLLAQSEYDQINVEYVRAKATLVKS
                     QSNLVLARQKMAETTVRSPIDGIVLSRTVSEGQIVASAVSNAGGGTTIARLAAMDRVY
                     LTAAVDEVDIGKIRVGQRGRIVADAYPGDVFFGEVVRVAAQSTVIQNVTTFDVILLVP
                     NRGNKLKAGMNATVTVDIAGKDHALLIGNDLLRTYAEVQADKKILEEAGVVLPEKRGD
                     RTQDSSGRGMGRKRMREQNSGSDSTRLRFVVLSEAGTLRAHPVRIGLSNADVSEVLSG
                     LDDSSRVMLVVYSQALKESERFKDRMKERTSFSR"
     gene            complement(27850..29220)
                     /locus_tag="PK937_04555"
     CDS             complement(27850..29220)
                     /locus_tag="PK937_04555"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNH31871.1"
                     /translation="MSWFLRLSAMLWMVGVVNGVAGGEDLLQAGPMLGYAEKREVMIW
                     VQTKKSAEVRIRYWSLEAVKTQDQKKISKISEPIRTAKESDFTAHIVLEALEPGTRYG
                     YEIVLDGKTPTLPYANSFQTQALFEWRTDAPDFTLAFGSCLYVNDPPYDRPGRAYGNT
                     SEIMRVIGNVKPDLMLWIGDNVYLREVDWNSRSGILYRNRHTRAIKELQPLLGNTHNY
                     AIWDDHDFGPNNSDRSYAHKDHTLDAFKLYWANPNYGLSESPGVFFQFEWQDIDFWML
                     DDRYHRAPDDMMDGPDKTMLGKTQLQWLKDGLLFSNAPFKVIVCGNQVLNANTTYESY
                     NFYKNERADLLKFIKDNGITGVVFLSGDIHHSELLKWEDPDFYTLYDFTSSPLTSGIS
                     SGDTVGTNPQLVDGTKITDAHTFGVIRFSGKRRDRVMTMETWDEHNKKRWSFSIHEND
                     LHPAKK"
     gene            complement(29305..31449)
                     /locus_tag="PK937_04560"
     CDS             complement(29305..31449)
                     /locus_tag="PK937_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH31872.1"
                     /translation="MPADTLNTTYRPRIGLVLSGGGARGMAHIGVLKILDELRIPVDV
                     IAGTSAGAIVGGMYASGMDVAEIEKFARDIDWKDMLNDKPSRDFRSFRKKTQENENLM
                     GFEVGFRSWNVVFPQGVIAGQKLSFTLKTITLPVSGIQDFDALPVPYRAVATDIATGE
                     RVVLRGGNLAEAMRASMSIPGVLAPVEINGRLLVDGMFVQNLPIDVAQAMGADVIIAV
                     DIGAPLMDRSHLNSVLGITAQVMNMVTTENVNQQAALLTNKDVLIRPDIGSVSVADYL
                     QADAIITDGEKAARQWIPMLRRFSVSDEIYNEFRKKIRTRRKTHDRIDFIRIGKNIGQ
                     DPRIYHRLKTQPGSSLNMKTLKADLTRIYALGDFEKVDFDIVTEDTSRGLLIDGRWKS
                     WGPNYLRFGLTMATDLEGHSSVNALADYTMTRRNQLGAEWKHLFRVGQNPTYFTEWYQ
                     PLDFKGNFFMAPYGRAQRFESGFYQQRVEIAQYNGYAAEAGHDFGIAFGNAGELRAGV
                     IWGHIDAHSTSNASRKSINAERSGIRIRTQIDMTDHPDFPKKGSIWQSAYFSSQKKWG
                     SDGAYQTLRGAGMVVHTWDRNSIMGAFEAGSDLGTSSPIYELSELGGFLRMSGYRPGQ
                     IKGRHFGMTRCMYYRRLSAPNASYTEGLYAGFSVETGNAWDNHSQITSRDLKFSGAVV
                     VGIDTVVGPLYLAYARAPHQEGIVYIMIGRPF"
     gene            complement(31518..32099)
                     /locus_tag="PK937_04565"
     CDS             complement(31518..32099)
                     /locus_tag="PK937_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNH31873.1"
                     /translation="MTLSTPLNAQSWSTGFFSGVNFSDARHSYRGTTTQGYARPVVGL
                     FVERNFVSWIGYRGELLWIRKKTDARTDSSYARWSATVIEAPLLMVLQSSSDYWGVSP
                     IFYFGVKVGYAVKTHYIEINGNATDFEGQTDYANRWSAAGELGAAIDYAISPKLSVDV
                     AFRFSLGLTDMDPNEPRWSFHDRLFTLGVKFKR"
     gene            complement(32257..33180)
                     /gene="mdh"
                     /locus_tag="PK937_04570"
     CDS             complement(32257..33180)
                     /gene="mdh"
                     /locus_tag="PK937_04570"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNH31874.1"
                     /translation="MKITVVGGGNVGATAAQRIAEKELANEVVLVDVVAGMPQGKSLD
                     MYESAPVERFDVRMVGTQGYDETAGSDIVLITAGLARKPGMSRDDLLMKNAEIIKGIT
                     EQIAKRSPNAIIIMVTNPLDVMTYVAWKVSGFPRERVIGMAGVLDSARFRSFIAMELN
                     VSVRDISAFVLGGHGDTMVPLPRYSTVAGIPLPDLMDKATIDRLVQRTRDGGAEIVKL
                     LGTGSAYYAPSSSAVEMIESIVKDQKRILPCAVWLQGEYGQKDVFCGVPIKIGSKGME
                     QIVQIKLTADEQAELNKSAQDVKDNIAKLTI"
     gene            complement(33318..33584)
                     /gene="rpmA"
                     /locus_tag="PK937_04575"
     CDS             complement(33318..33584)
                     /gene="rpmA"
                     /locus_tag="PK937_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNH31875.1"
                     /translation="MAHKKGLGSTKNGRDSHGQRLGVKRYAGQTVLAGNILVRQRGTK
                     FHPGRNVGIGSDDTLFALIDGVVKFEHKDKTRLRVSVYPQEAKA"
     gene            complement(33595..33909)
                     /gene="rplU"
                     /locus_tag="PK937_04580"
     CDS             complement(33595..33909)
                     /gene="rplU"
                     /locus_tag="PK937_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNH31876.1"
                     /translation="MYAVVDIKGFQYKCEAGKSLVIPRVAEAEVGDKIVFENVLLISE
                     NGAAQVGQPTVKGAKVETTVIAHGKGDKVIHYRKYRRKGFQKKKGHRQPFTEVEVTAI
                     TK"
     gene            complement(34035..35141)
                     /locus_tag="PK937_04585"
     CDS             complement(34035..35141)
                     /locus_tag="PK937_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNH31877.1"
                     /translation="MKSVSTYVKKTTRWYTRYQNVISPVSFLLGFVWDAFSSKRIDAM
                     DQNYLLMGYLFIAGILLLLLHAVKAEKIKTGFWVTYQKLLPLGFHFCLGNLYSNHVYF
                     YFQSAAGFKSLVFIAGLMVLLLANEFYKERIHQFYLQFVMFFLAAFSFFIFFVPLFTR
                     ELGYRSFLIGGCISLVFTMMLIFAVALIARARYRKLLIKSAGLMIVIYGVMNIFYFAR
                     WIPPVPLALRHVGIYHTMKKKDQRYELQYEKAEAWEFWRTSGDVYHWQPGDTVICFAA
                     IFAPTEIHTTIHYQWEQYLDDRNAWVLQDHQSHTLQGGRDAGYRGYSRKGVISPGAWR
                     VEITTEEGLLLGSQTFRVERMPMNYDRKLHTVVW"
     gene            35231..35503
                     /locus_tag="PK937_04590"
     CDS             35231..35503
                     /locus_tag="PK937_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNH31878.1"
                     /translation="MARMVHCIKLNQELEGLDKAPIPGEIGKRVYEQVSKQAWKMFEE
                     HFKMVMNEYRLNLMDPRTDEIFKQQVQQFLFSGTAARPEGFVEPTS"
     gene            complement(35510..35704)
                     /locus_tag="PK937_04595"
     CDS             complement(35510..35704)
                     /locus_tag="PK937_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31879.1"
                     /translation="MIRFTKRFYFGMPLSLAGILIAFNYISNYRTYAVVLFWTGIITG
                     IVGCYLMFKGIKSKKIKIEP"
     gene            complement(35701..36648)
                     /locus_tag="PK937_04600"
     CDS             complement(35701..36648)
                     /locus_tag="PK937_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNH31880.1"
                     /translation="MIPFKNTHTMTVDEFAHVMENLRSYGAYVGFISGGETTIVDGLS
                     EMLLEAHKTFRFATTLVTGLYNKWENIEPAARVCMENGMHIQTSFDGLGEVGDHLRGC
                     KDFETVISENMRRISELRRDIQKKNKKKSVLYANTVMSDINLDQVPDIIAHVKSLGWK
                     STVGMYHTLTFSTRYNDDMVLRPGDRLNKTMTFLKQNPDVMNLDAWIDGVIPYVEDPN
                     LNWCPFVKAPYLATRTTIMENGDVHLCKGNPIGNVFRQSLEKIFESYEYKQRLDEYSR
                     CEGCWTTCYTQRYLSVRPKSISEAVSNIKKIKRNTTVEL"
     gene            complement(37141..37581)
                     /locus_tag="PK937_04605"
     CDS             complement(37141..37581)
                     /locus_tag="PK937_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNH31881.1"
                     /translation="MKSFLFFADNNWLLLILRVTMGFVMLPHGAQKMFGWFGGYGFSV
                     TVNFFTETMRLPWLIAVLVILIEFFGAIGLILGFGTRVCAMSFIVIMLGAIVTTNYHH
                     GFFMNWFGNQQGEGFEYHLLVIGICAALLISGGGDYSLDRLLHG"
     gene            complement(37667..>38086)
                     /locus_tag="PK937_04610"
     CDS             complement(37667..>38086)
                     /locus_tag="PK937_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH31882.1"
                     /translation="GCFKVCYSDDTGTEFIVKFALENWWVVDLESFINQTPSFFYIQA
                     IEDSKVLQLSRSNYDKLLRKIPAFHKFSNERWQNGFIALQHRIMHDQSMTAEERYRRF
                     KEKYPTLEQRIPQKLIAAYLGITSEFLSMIRKKILIS"
BASE COUNT        10593 a         9246 c         7824 g        10423 t
ORIGIN      
        1 ccaatgaccg accactgggg aataattagg aacaaaatgc gatttataca aaaattccgg
       61 atcagaaaat tcccgcgtat aaggatattc gcccaaatat cggtagtaac ttcccaggta
      121 gatggtcatt ccaccaaatt gaatcagcag accgatcacc gcaagcgttt gtatcataaa
      181 acgcaaagtt cggttttttt taatagtttc atatgcgtaa gttcctatga ctataaaaag
      241 caaaggaact atcggaagaa gatatcgcgg cccccaactc ccgtcaccgg cccacgctac
      301 aaatttagcg tggagcgcta tgaatgtgca gcacactacc gttattgctg taaataattt
      361 gaactctctt tgtaaaaaac aacgtgccgc cgggaatata agtatggtaa gcggtgcgta
      421 gagaaataaa ctttttccgg aactaaaaag taatccataa accccgacta aaaaaggatt
      481 tgaaaaaggg ttttgtattt tggaataacc cgatgtaaaa ggcccaccga aaattaacca
      541 attgtagcat acggccattc cgataaaaac actcacccag gcactgaaga tacttaccgg
      601 tataaccaca tttgccgctc gcaaccaatc cttaacatga agacggttat catataaaac
      661 ccatactaga tataaaccaa aaaccggtac aaaaatcaaa aatgaaggtt tgatcaatac
      721 aagccaacct gcagcaattc cggccaaatg cagatgcttt cgcaatgaag ttcgaccaaa
      781 tgtaaaaatt tccattatgg ccatcagtac gcatactgtt atcaggggtt cgcgcatcga
      841 agatttagaa tacacaaaca cattagttgt aaacaacaac gccgatgccc ataagaaagc
      901 gaaaccccag gatacgctca tgaaacgtag gtaatgtatc atcaaaaggc cgagcagagc
      961 acataagatc ggattgatca gcgtgaaaat gcccttgaga acaaaaagcg aatacgtttc
     1021 aggaattgga agtgctttgg ttatcaataa gccgaaggca tacatcggtg cacccagtat
     1081 cgtataaccg acaccacctc cgacataaaa tcggccatcc acgccgaatg cgccgttatt
     1141 gacgatactg gtcggtatgg ccagcgttcc cctctcgact acactttgcg cttcgagaaa
     1201 atatgccgtc tcatcgctgg atgcaatcct cccgcccgtg aagagtaaat tcacagaaaa
     1261 taaaaacaag aaaatatata gatacgtttt tttcatacga cgcgtctaat ttcgtctgtc
     1321 atttgcgctg ctttgattga ccaactgttt ttcgcagcta aagatttaag cttttctgta
     1381 tctgccttac tacgtagagc tttgccaaca tattgaacaa agtcttcgtc ggaatccgca
     1441 atataaacat gtggagctaa attacgaact tcttcagaga agccaaacgt tacagttggt
     1501 ttaccgcagg ctagaaattc atagagggta tttgggtttg ccccccaaat acgacggtta
     1561 ttgcggaatg gtaagataca cacgcttgca ctgtgcatat acgaaggtag ttcagcatgt
     1621 tttttttctg agatcaatat caacccgtaa acttgagcca gtatccgtag ctgctcaccg
     1681 gtaaccgcat gtttgatcgg gcctaatacg acaaatgccg cctcagggag tgattcgatc
     1741 agtcgtgtta ataattcaac attgaaccat tctgctacag ctccgctaaa aaatactaca
     1801 ggtcggtctg tgggcatttc caccggttta atagcaggct tcgaatcaaa taatttaaaa
     1861 tctacaccgt ttccgataac cgacacacgc gatggattat gaggtatgat gtcttctact
     1921 aattttttgg aaaccgtcgt agtaacattc gaaagtcgaa tggttttttt aaaaaattcc
     1981 tttgcggttg ggagtaatag agcaaacccc aacggataat cattgcaatc gtaaatcaca
     2041 gccttttttt tcatgcgtcg aatgatatcg gcatagtaaa tattggatgt tgcaatgacc
     2101 ctttccggcg catcaaatcc tgtttttcga cataaccaaa acacccacaa tgacgctatc
     2161 cattcgataa ccgtacgaaa tggccaaagc acttgcaact tgttaatcag agggtaaggc
     2221 gtagctttaa aaaaaggcac ggtggcataa gtgacccgcc cctcacgaac aggttgaaat
     2281 tgattatttt ttcctaacac aaaactttcg ataaatagca cttcccattc cggtggaaaa
     2341 ttttcaatga tctgctgctt acgcgttttg agatacgtcc attttatttc tgaaaaccaa
     2401 acaactttca tagcatcatc atcctgcaca aatacctatt ggcaactgtc ggccatttaa
     2461 aaaatcagtt actttcattc gttttttgga cggaggctgc aattccaaaa tcgcgaccac
     2521 tccatcaccg gtttgaatcc atattccgtc ttgtttatta gagactatga tcgtaccggg
     2581 agccgactgc tcggctgttt tgaccgtttc aatacgaaat attttgtacg cttcaccatc
     2641 aatggtcgtc catgcgcacg gatagggaga tagtccccgc accgtatttt ttatttctcg
     2701 tgacgatctg gtccagtcaa tacggcatgt ttctttgaat agtttaggtg ccgttgtcgc
     2761 caattgatca tcctgcacgc gcgtcaccac cttattctcc gcgataaggt cagcggtttc
     2821 taaaaacacg tccgcgccta tttcggataa acgatcatgc aattcgccca ccgtttcatt
     2881 ttcaccaatg atggttttgc gttgaagaat aatcgtaccg gtatccactt ttttctgaat
     2941 aaaaaaagta gtgactccgg tttcggtttc accgcgcatg atcgcccaat tgattggtgc
     3001 cgcaccgcgg tatttgggca gtaaagaagc gtgcaaattg atgcacccca aggacggcaa
     3061 ttcaaaaact tccggcggta atattcgata cgctacgact aaaaacacat ccgcattata
     3121 agaacgtaaa gacgcaatga attgctcatc tttgagattg tcaggttgaa gtacaggcag
     3181 acccagttcg acggccaatt ctttgatggg cgtaaaactc acttgctgac cgcgaccgcg
     3241 aggaagatct ggcgtcgtaa caacagctac gacttcgtga tgactttggg ctagtcggcg
     3301 gagcgaagga acggcaaatg ccgcagttcc cataaataca atgcgcagtt ttttcatagc
     3361 gcggcaaagg tagcatgttt tacgggaaaa atgaagcaga ttttacagag tgcgcagatt
     3421 atcttcgtta gaatagtatt gcgtatgaca agcagtaatc aagccaatca tgtcattatg
     3481 tgcgaataaa tcaaaatttg aaacatcaga ttatactctg ataatgtgat ttttatcagg
     3541 cgaatctgtt aacgaaacta ccataaacca atttgaacat tgtcacaatt gacgacaaaa
     3601 gccttctctg taggcttcta cagcgatttc gcctttgttt gtatctataa cctatattga
     3661 ttgtgtagcg actgtttgtg atttctgatt atttgagtac tgacggtgat cataggaatg
     3721 cctgacaata tgagctacta ttataattcc atataatatg taatcgtctt atcaataacg
     3781 gagttatcat gagtttacct ttaattatcc aaggtgggat gggtgttgcc atttccgact
     3841 ggcggttggc cagagctgtt tctatgaccg gtcagttagg tgttgtgtcc ggcaccggga
     3901 tcagccgtat attcacttca cggcttatgc agggcgatgt gtcgggtatg atgcgcgaag
     3961 cgctgtctca ttttccctct aaggaatccg tacaatccgt tctcgaacgc tatttcatct
     4021 ctgggggaaa atccgcagag gaaccttata aatcacagcc ggtttactcg ttaaatccgt
     4081 ctgttttttt ggagcaacta acaacattag ccaattatgt agaagttttc cttgctaaac
     4141 gtgaacatac aggcattgtc ggaatcaacc ttcttgaaaa agtgcaaatg cctaacctcg
     4201 cttcgctcta tggagctatg ctggcaggcg ttgactatgt attgatggga gcaggaattc
     4261 cgactcaaat tgcaggaata ttggacaaac tcagaaatca cgaacctgtc agttatcgct
     4321 tagacgttca aaatgccgct cccaacgatg atattcgttt tcattttgat ccgcgtaaaa
     4381 tttttcccaa catggcaaaa ttgacaggcg ccctcaaacg tcccaaattt attccgatcg
     4441 tttcatccac agtactcgcg caagctttga tcaaacgaag cgaaggtaaa atcgatggct
     4501 ttatcgtcga aacgccgacg gccggaggcc ataatgcacc accgcgcgga ccgatgcgat
     4561 taaatgaacg gggagagccg atatacggag aaaaagataa agtagatatt gcgaagctaa
     4621 aagaattagg tctgcctttt tggttagccg gaggtttcgg gcatccggaa aaactgaaag
     4681 aagctataaa taatggcgcc gcaggaattc aagtcggcac tttattttca ttatgcgatg
     4741 aatccggaat ggatgcgcaa cttaaaaaac aaactttgca aaatgtgctt gataaaaatt
     4801 cacaaataat tactaatccc gttatttccc ctaccgggtt tcctttcaaa gtcacacaac
     4861 tcaaaggtac agtgtcagat cctgaagtat attccgctcg cgagcgtatc tgcgacttga
     4921 gccttttgcg aacggtctcc aaaaacgacg acggaactat cgtctatcgc tgtcccgccg
     4981 agccggtgga agaatatgta cgcaaaggag gcaatgtcga agatacgaat ggccgcactt
     5041 gtctctgcaa taatctggtg gccgctgccg gatttccgca agttcgaaaa aacggctatg
     5101 tcgaaccgcc tctggtgaca tctggagatg atttaggaag tgtgaaaaac ttcatcacaa
     5161 aagataaatt acactattcc gccaaagaag ttatccatta ccttacagaa caaaacaatc
     5221 attaatcgaa tgtagaaata ctgactctat taagagtatc aacaaaaaac gaggttcagc
     5281 aacctcgttt ttttatgcaa agtcgtaacg attttttgat ttaaaaatgg tttgtgttct
     5341 tcacaaaact aatgatacta atgaaaatgc aaatttgtgc gaaatcataa gtcactttga
     5401 cttttgtcga agtgactcga tctgaataaa aacatcttcg ggcatcggcc tatgaccttt
     5461 atcctgtatt acgtgttcgg ttatattgac ataaccctga cgttgtgcta aagttttacc
     5521 ttcaagccac tgatcgtaga ggcgtccgcc ggatgaccag gaagaatcca aactgccgac
     5581 gaggttgatc agtggcaaat tacgatgttc ccctgcaacc ggaagtatcg cttcacccag
     5641 gcaacggctc ctaaaattac cggcaacgac aacgatggat ttgatttttt caggctgaag
     5701 aaaagtcatc gcccacgcca aatgagcgcc ggcttcaaag cccgtaatat gtatgcgcgc
     5761 ttcgccgttg tatttttgag ttacatcttt caatacggca tcgagacctt gcaagtcaaa
     5821 agtgcaagtt ccttcgcgat caatttgatc ccatgtgtct gatgtataag gaaatatttc
     5881 aggattacgc agaccctgat tcccattcgt tacgataaac ggtgccacga tgatgtaagg
     5941 tcgttcgccg cgcgctttta tgaaacgaag tgcattttct ttgtactgtt tttctgcggc
     6001 ttcgcagact accagtactg gccacgtttt tgtattactc caccctttgg gcaatgatac
     6061 ataatactgc attgcatgat ctttagcttt caagagcaaa agatcttctt gctgagcctt
     6121 gacatcaatc aaaactaaaa agaaagttac atacagaaat gcagtcgaat tttttaaaag
     6181 atttaaactc atatatttca ttttataata aacactaccc cgccaagttt gacggggtag
     6241 ctcgtttaaa aaaaaatcga aaggacaaat cattaaatcc ttacatatct gtcaaagcgc
     6301 tattttcctt taaaaggctt caaccaccac gaatgaaatt gttgttgttc cgctgagggt
     6361 tcactaacag gagttattac atgattttcc atgatttcaa ttttaccggc tgtaccttgc
     6421 aacatcattt ctttggtatc gcgctcgatt ttccacgcaa aggattcgcc gggttgcatg
     6481 gtttggcgaa tttgctgcat cgcaggatcg cgcaaatcat aaagcttatc ctgataccac
     6541 atccccaata tcacatctcc ttcgcgtatt cccgtttggc gattaacttc atcggtcgta
     6601 tcaacaccgc taaccacaac tttatcgtgg tctaacgaca acgagaaggt acctcgcacg
     6661 gaacgaaatc tacccgtagg ttttgattta acgtaatcat atcccgcttt tgcaatgtat
     6721 tcactcaccg gtagcggctc gttaccctgg atatatcgtt taataaaatc atcgatttca
     6781 gggtacgtca tcttgacgtt gatttcaaaa aattctgaat ccggaaaagc tttatccggt
     6841 ccgtattttt tggaaagctt caatacgaca tcgcgcaatc ccgttttacc cttcgaaagc
     6901 tctaacaatc gcatatcgag taacatggcc gtcaaagcgc ctttggcata gatattttga
     6961 tactgcgcgt ttaagtgccc atacgaacct aaggatagat ccaccaagct gacgcgcgga
     7021 tcataattgt cgttgacact cattttttga gaaactctct cacagaactc atttggttga
     7081 actaatccgc tgcgcacttg catgatttgt gcagcccatt ccgtaacccc ttcatagaac
     7141 cacaaatggc gtgatggtgt gggttgttca aaattaaatc gttcgatgat ttcgctgtga
     7201 atgttaagcg gcgtgacaat gtggaaaaat tcatgcgccg ccgtacttct aatgatatcc
     7261 gcaagcccgg accccatgcc tccgcctgaa ggaaaagaaa tgctctccgg tagaatgtaa
     7321 aacgaggaat aactgtgttc ccatgcaccc caagccggca aaggatctga ggtaaactta
     7381 tataagaagg cataacgttt gaccggcagt cctcctagaa attgctctgc tgatatcaaa
     7441 atatctttaa ggtgcgaaag cagactgtca gccgccatcg ttccattttt ggcatgacaa
     7501 aatacatcca cctgagctcc gccgattttc ataccggctt tagtcagctt accaaacaaa
     7561 aacggagagt caaccacttc atcgaaagtt ttagctttat aggttccgtt tttttcttca
     7621 agcgctgtac cgaccagcca tccttgggga taattgtatt tgatctgcat cggatttgac
     7681 tgaaagccct gaaaatatcc gaaaaccatc tgaccgttga gtaaaacatt atccgtttcg
     7741 atcgaagatc cgctcatcgg acccacttga tcttctttga ttattgtatc atacgaatcc
     7801 tcgacttgat atcggatcgt atgtagtttt ttggcacccg cgatgacata ctgattaacg
     7861 ctgcgatgta taaccggcaa cacacgccct ttttcatcat acgccgtaaa accgccgaca
     7921 aaacgaccga tatccattac ttggtacgtg ccaggtgccg tcgaggcaaa ctgaaacacc
     7981 gtcgtgtcgt tttgaattcc tttaacgtgc aacgtaacaa aaaaagaatc tttttctatt
     8041 ttggtcaaat caacatcgta tcgcaaatct agtttatctc cggcaagtac ggaacctgcg
     8101 gttattataa tcgatgcaat ggcgcttaat attttcacct tcgtctcctg attctgtaga
     8161 ttaattactt agccaacgtt tacgaaatgc caattggtcc gacgtcggtt tctcgacggg
     8221 aagtatgaca tggggcttga tatttttttt gacaccggcc ttagctgtca gtttgacctc
     8281 ttcactgcca cgttgtacga ttaagccaaa ttcatcgccg ggcttaacac tattcattgc
     8341 tttttgtacg cccggtttgc ctaaacgaaa ttccgtacca tctttaatga agcccaggaa
     8401 agcatcgccg ttttgcaagc ccagtttggc cgtcacggca ttggatgcat ccacgtcttt
     8461 aaatgttatc ttatccgtac cataatcaaa atcaaacttg cccatgtccg ttttttcttc
     8521 attcgtttca aactttgcat gataggcata acccgccaaa ttcagcatac tttctacggg
     8581 caatggttcg tatcctttga tataacgatc aataaaatct ttcatgtcgg ggtgcgtcat
     8641 tttgacgaca atgtcaaaaa tttctttttc cggaaatgct tttttcggtc cgtactgctt
     8701 ggccagtttt ttaaccaaat catttaaacc cattttaccc ttggtgagtt cgagcagtcg
     8761 catatcaaaa agcatcatcg tcagtgcacc acggatgtaa atattaccgt actgcggtgc
     8821 cagatcacca aacgatccgc gtgcaagatc cactaaactg agatctgaac gaaaataatc
     8881 actggtaact aatttattcg taatacgctc tttaaattca tccgaagtaa tcatgccgcc
     8941 gcgcacttgc atgagatggg aagcccattc cgtcgttgcc tcgtacagcc aaagatgatc
     9001 ggaaggcgtc ggttttacaa aattgaattg ctggataatt tcactgtgaa tattgagcgg
     9061 tgttacaata tgaaaaaatt catgcgcgga aaaatctttc accgtttcac cataaacact
     9121 catcggtttt tcaggcataa cgtattctga ggaatagcta tgttcccatg caccataagc
     9181 cgccggtacg tcttgacgaa aatgatataa aaacgcatac cgctttacag gcaattcacc
     9241 taaaaatttt tccgcagcga ctagcatcgc ttgcaatgct tcaagcagcg aatcggccgt
     9301 aacgatatta ttttgcgaat aacagtagat acctacttca gcaccaccaa ccttggtttt
     9361 cacttcggtc aatcgcccta ataaaaacgg cgaatctacg atgtgatcaa acgaatcggc
     9421 ggtgtataaa ccgtttttgg gagttagcgc ggttgcgacc ttccaatctt ttggacagtc
     9481 aatttgcact tgtatcggat tagcctgata cccgtgaaag tatccaaaaa ccatctggcc
     9541 gttaataagt gcattgtctt tttcaatatt ggatccggcc atcggataaa tgggatgaac
     9601 ggctaaattg gtgtcattaa catcttctac gcgatacgat atcgtggcca aatttttagc
     9661 gtcattgatt acatattgat taatgccgcg ttgtacgaca ggaagcgatt ttcctttgct
     9721 gtcgctggcc gtaaaaccat tgacaaagcg accgacatcc atcacctgat atgtaccggg
     9781 cgctgtcgaa gcgaattgaa ataccgccga atcatttgac catcctttta caaccagcgt
     9841 cacataaaac cgatcttgca taggttcgga aatgtccaca cggtaattca gtttgatatc
     9901 ccctgcgata agcgacccgg aaaaggtaac agccattacc catagtttca tccaacgcat
     9961 cgaacgtctc cttaataata aaatgatcag atcgtatcaa atgcttgaaa tacgttaatg
    10021 gttacgaaat gtttctttgt ttttttaaaa taccggattc aggggatatg gagccaaatg
    10081 cggcggaaac gaagctgttc cggagtggga tcggatatgg gacgaatacg atgtttttcg
    10141 ataacggggg cacgaccggc taaggcattt aatgtcaacg tttgcgaacc gcgacgaaca
    10201 acccaggcaa acgatgcgcc ttcgtctata aatgagaaaa attcaataag cgaaacatcg
    10261 gtcaaaggca gatcaatgtt attaagtacc attttttcga tgatgtcgcc agcttgcaaa
    10321 cccaatcggg tacaaaccga atcgttggga tcaacggaag taacaattac catcgtgtct
    10381 ttttgcatca cttcaaaaag cccaaggtga ttacgcatcc gcgttgtttc atacttggat
    10441 tcgtattgat aacctgcctt gataagatat tccgctatag gtagccgctc cgatccgcga
    10501 atgtagcgat ccatgaattc acgcacctcc ggataggtgt gatcaacaat gataccgtat
    10561 aaggaactgt cggtaaaaga tttccccgca ccatattttt ttacaagtgc catgatcaca
    10621 tcgcgaagac cgcgtttacc gtcggaaagt tccaataatt tcatatccaa tagtgcggct
    10681 gtaagcgcac cgcgttcgta gatattagca tattgatctt catacacatc atacgcgccg
    10741 aggctcatcg tttcgagact gatatccggc ttgtatcgat cgttgcgctg tagtttttcg
    10801 cgtaagcgct gcatatatac actgtcgggg atgactccgg cgcgtacctg catgatatca
    10861 ctggcccact cggtaacgcc ttcataaagc cagagatgac gcgagggtgt cggacgatca
    10921 aagcgatacg ggacgatttt ttcactatgc aaattgagcg gcgtgatgat atgaaaaaac
    10981 tcatgtgccg ctatgctttg tacgatgggc cgaaggcctt cgagtttcat ttccgggatg
    11041 acataaaacg acgaataatt atgctccagc gcgccgtata tcggcccggt atcttcacga
    11101 aaatggaata aaaaaacata acgatctacc ggtaatgtgc caataaattt ttttgatgca
    11161 ttcagtatat ctttgagttg cgagcgtaac caggtcgcgt taacacgatt tttttcagaa
    11221 taacaataaa ccgatatctt ggttttatcc attgtccaat gagccgtcgt aaggcgaccg
    11281 gccataagcg gcgcatctac caaatcatcg taagtcggtg ctatgaggta accgtcttta
    11341 gaaactaacg ccgtacccaa tttccaatca gcaggcatcg taacgcgtaa acgatatgga
    11401 atattttgaa attccgaaaa gtacccgaat gtcatgaacc cgttgatgac gatattgtcc
    11461 ggctctatat tcgtgccacc catcggatgt atggggtttt caccgattcg ttcaccgaag
    11521 ctgtcattaa ccttgtacac aatgcgtgca aggcgccgtg cttcgtctat acgaaattga
    11581 tttaccgacg tacgtgtgac ggtaagttcg tgccctgatg tgtccaacgc ggagaaactt
    11641 cctacaaaac ggcctgcatc caatatcgca tacgtaccgg gcgccgtcat cgggaactga
    11701 aaaagtgcgg atgaatgggg atattttttt atatcaagct gaacaaaaaa cgaatcgtcg
    11761 tccaatgaag tcagatccat gcggtaagtc aactcaggta attgcgcaaa tgccgaaaca
    11821 gctttgtgcg caaatataat ccataagaaa aatgcccgcc gcgtcatgaa atcctcaagc
    11881 cgtttgcaac aggtcgctcg gtttgaagca atacaacccc ttccggtgtt accacgccga
    11941 gcacaagaca ttccgaaaag aaattggcaa tttgtttggg tgggaaattg acaacagcga
    12001 ggatttgttt tccgatgagg gcttgtttgt catacatatc cgtgatctgt gcactcgagc
    12061 gtttgatgcc tgccactcca aaatcaattg tgagttgata agcgggcttc cgtgctttgt
    12121 gaaaatcatt tacttcgatg atcgtaccgg tgcgaatttc caccttgtca aaatcgtcgt
    12181 agctgatcat agttttccgc tcagataggc taatccctga atacccaaat cgggtttgat
    12241 ttcatgaata gtgcgcgaaa ctttggcaaa tttggcgatc atgcctccgc tggccaaaac
    12301 aggcatatcc ggttttccta attgctcacg catacgcgtt acaagcccgt ctatcatacc
    12361 cgcataacca aagatcacac cgctgcgcac cgcatcatca gtattttgac cgatcggcga
    12421 attcggtaca tccaaataaa ctgttttaag taatgccgct tttgcaatca atgattcgac
    12481 cgaacctttg atacccggcg aaatagcacc gccttgaaaa acaccatcgc tcacggcaaa
    12541 aaccgtcgtc gccgtgcctg cggaaacaat gatgacatct tttcctttca gacgaaccgc
    12601 cgcttcggct aatatcaaga tatccgcacc aacctgatga ttgattgccg gatgaaatcc
    12661 ataactcaaa ggtgacttaa catcaataaa agatgatttc actccacggg tttcttccaa
    12721 taactgaaac gctatggtga ccgacggcac gacactggca atcagcagct ccggtatcgt
    12781 atccaatttg gcctcacggc aaacattctg aagttgaaaa taatattcat caacggagcg
    12841 ttccgaatcc gtacgaaacc gccatacgtc cacaacacgc tcgccgtcca gcacgccgat
    12901 tttgatatgc gtattcccga cgtcaattcc cagtcttgtg ctcatacttg cactcccaat
    12961 ttttttccga ccgcttgaat aaattcaaat ttgccttgtg cgtacgtatt tttatcttca
    13021 ggtacgtttt cagataattt attttttaaa tccgcatacg catcccgcaa agccttatca
    13081 cggcgcaaaa catcacgaaa cgtaatatga cgtccccaaa acggcgacgt tctgacagcc
    13141 gcatgaatat gaaataaatg atcgcgtgct ccgtaatccg gattccaatt tatggatttc
    13201 tcctcgacat tgttttttgg tctaataaaa aaacgtcttt ctggaatata tttttcgtac
    13261 tccggtatat accgataatc cgcatccgtc aatttagaca ccatcgcttg gagttcgatc
    13321 atatcgtcag ttccgatcaa aatatccacg acaggttttg ccgctaaacc cggtacagcg
    13381 gtacttccga tatgttcgat actcaaggtc ttatcaggcc aaaatttcgt taaccgccgg
    13441 gattcggatt caaataggac agcccactgc gcatcatacg cgataacatg aattttcaat
    13501 gatatagtac agataaggtt ataggaaagt gaatatcccg tattgcaata aacataacca
    13561 atgcgtatta ttttttcaaa caataacagg aatgatttct gatgaaattt ttaatactca
    13621 atggccccaa tcttaatctg cttggcgagc gcgaaccgca catctacggg actcagactt
    13681 tagcagatat agaggataat ctcaaaaccg tttttccgga acatacgctc gaattcaagc
    13741 aatccaacat ggaaggggaa ttaattgatt tattacacgc tgtgcgtaaa acgcacaacg
    13801 gcgtggtttt taatccgggg gcatattcgc attattctat cgcgttacag gacgcaatta
    13861 catccataca aattcccgtc gtcgaggtgc atttatccaa tattcatgca cgcgaagaat
    13921 ttcgcagccg cagcgtagtg gcccgggcat gcatcggtca gatcaccggt ttcggtagct
    13981 atgggtacga gatggccatt cgtacgctga tacagcatca cacaaagtaa tcgatagata
    14041 tcgcattatt ttacaatctc aggccgcctc tgttattaca aattccgctt ccacgatcac
    14101 ttcggttttc atagaattgg caatcaggca ttttttatgt gcatcatgca tgacggagcg
    14161 cgctttttct aaatccgctt cgttatctac agcaattttg gggcgcacta tgatttttgt
    14221 aaaccgataa ccgccttcaa caaattctaa aataccgttg gcccggcttt gataatcggc
    14281 aatgtgtaat ccggcttttt ccgtaaatga aataaacgtc gtcatggtac aaatttcagc
    14341 cgccgcgacg aacaaatctt ccggcgtcca gacatcggtt tcacctttaa actccggcgg
    14401 gctggcaaca cgaaaagccg gtttatcttc ggatgaaagc atcgctgcac gcgttgctat
    14461 acgatgcaaa ccggtatgat aggtaaatgt tttatatttg ggtttggggg ctacagcttc
    14521 cataatgcgc ctctataata ttggaatagt tttttatttg tatataacta tatagatata
    14581 tagagatcaa tcaaatgact tttatcataa aaaaacccgc caaaaatatc tggcgggttt
    14641 tatagaatct ttatgaaaga taattatttt ttcttcttct tatctttctt tttgtctttt
    14701 ttcttatccg attttttaga cttcttatcc gatttcttat cttttttctt gtccattttt
    14761 ttcgcttcaa cttttacggc aggttttgcc gaagtcgtac tttttacttt aatgccaagc
    14821 acctgcgcta cggtcacgcc gagatcggca gggcttttga cgacgtgaat tccagccgac
    14881 ttcagcgctt tcattttttc ttccgctgta cctttgccgc cgctgatgat cgcgccggca
    14941 tggcccatac gacgccccgg aggcgcggtc tggcccgcga taaatgcaac cacgggtttt
    15001 ttaaaattct ttttaaccca tgcggcggct tcttcttcag ccgtaccgcc gatttcaccg
    15061 atcataatga tcgcatccgt atctttatct tcggcaaata gtttgaacgc atccacgaaa
    15121 cgcgtaccga tcacaggatc accgccgata ccgatacagg tggattgacc gattttcaat
    15181 gccgttagct gtcccaccgc ttcataggtc agtgttccgg aacgggaaat aactccgacg
    15241 cgaccgggtt tgtgaataaa tgccggcatg ataccgactt tacatttacc gggcgaaatc
    15301 acaccggggc aattcggccc gacgagatgc acacctttat ctttgatgaa attgtaggca
    15361 cgcaccatat ccgcggcagg gattccttcg gtaatacaaa taatcacttt gatacctgct
    15421 tcggctgctt cgattatggc atcggcagca aaagccggag gaacaaagat caccgatgta
    15481 ttggccttgg ctttttccac cgcatctttg accgagttaa aaacgggtac accatacttc
    15541 agtttatctt tttcatttcc tttatataca agaccaccct tgcccggtgt cacaccggcg
    15601 actacgtacg gaccatactc tgtcatctga cttgtatgaa aagtaccttc tttacccgta
    15661 ataccttgta ccacaagacg ggtcttttta tctaccaaaa cactcatacc attctcctca
    15721 aagctgtatt gatcaatata ttcttacgac tatttgcaaa attacattgg cccaaatacc
    15781 ggcaagtaca tcatccatca tgacgcccca tcccttcggt aagtcctgca ggcgattaat
    15841 gccaaaccat ttggtaatgt caaaaaaacg gaaaagaaca aaagcgccga tcatcacaat
    15901 ccacgttttc ggaagaaacc ataaggcgat ccaaagtcca acgacttcat caatgacaac
    15961 acacgaagga tcgtgcccgt acaccttttc cagacgcgtc gcggagacca cgccaagccc
    16021 gaaaactgcg gcgataataa taaaaaaaac agcgtgaatc aataaggaag atgacgaagg
    16081 aaaaaaaatg aagtatatag ccagtgccaa aagacttccc caagtacccg gcgctttcgg
    16141 aagataaccc acccccaacg ccgtacccag gagcttatgc gcattcagca tcggatttac
    16201 gtgtttttga cttgtgaagg tgaatgaaga gaacgaaggc tttcacggtt ttcatacaaa
    16261 tacaaaagac cggatatcac ggtgaccgcc gtcaccacca gcatgcctac atagatcact
    16321 tcgtagtgcg tgacgatgta gatgaaataa tttaactgct cactgatcgt catatgtatc
    16381 aacgactgca tggcaataaa aattaaaatc aatccgatca tcaacatctg aaaggccgtt
    16441 ttgagcttgg caaaaaaatt agtcatcatc ggtttaccgc gacgtatcgc atacgaacgg
    16501 aggctggtta tcataaaatc gcggatcaca atcaccatca ccatccatag ctttacaaat
    16561 ccgagataaa cgaatgaaaa aaatgccgat aataccagta ttttatcagc caaaggatca
    16621 aggaatttac ccaatccaga atggtaactg tatttgcgcg ccaaataacc atctacccaa
    16681 tcggtcaccg atgcaaaaac aaaaagagca acggagtaaa cctgatactc atcggacagc
    16741 aaacaaaaaa caaataccgg agttagcagg atgcgaagga tagttaatat gttaggtata
    16801 aaacgcatgg ctaagataaa cattgtttgg ataaccggtg ttggctatct tggtggcata
    16861 ttactctaaa agcgttcaac aatcaagtta tttctgtgcg taaaacatgg cagtggatag
    16921 atacggggaa gcagcccgga agtttcaaca tggcttttga cgaatattta gcggcagaag
    16981 tcggctccgg tttgcggccg accactttac gttttttcca atggcaaccg tattgcattt
    17041 cactgggcaa acatcagaat atatccgaaa tcaattccga aaaagcgcat cacgacggac
    17101 ttgaaattgt ctttcggccg acgggtgggc gtgccatact ccatgcacaa gaattgacct
    17161 acagtattgt ctatcatgac gatatccgcg gttcggtcga agaatcccac gcgcatatca
    17221 gcacggcgat tgctattgct ttgcgccgat tgggatatga agcctattgt acgaccgagc
    17281 agactgattt caagcagcac taccgaaatc cctcttccgc gttgtgtttc tccagttccg
    17341 ctaaatcaga aattcagatt gagggaaaaa aagtcgttgg cagtgcgcag cggcgatatg
    17401 cgcaggctac gctgcagcat ggttcgatcc taatcggaga ttttcaccga aagctggccg
    17461 attatatcca tgccgacgcg gctactaaaa atattttgca acgcgttatg aatgaaaaaa
    17521 ctaccgagct gaatcagtac atagccgtgg atattgctgc tctcaaagaa tcgctccggg
    17581 aagctttttc tgaatttttt tcgatagatt ggacagatgg cgcgtggcaa gccgatgcga
    17641 tcgcatcaca tcatcagcgc tccgctttca tggtgtgaag acgccgcaac agccgcatat
    17701 tttcgaagct gattcaatgt actttgcagg tctttgggca aagacgctga aacagtaatt
    17761 ggcctgtttt cccatggatg caaaaaagta agcgctgcag cgtgaagcgc caatcttccc
    17821 ggcaaggctt tttcatccga atcgccggtt ctcttatagt tttttttcaa ctgcgataaa
    17881 aatattccgg tctcactacc gtaatgcata tccccgacga tcggcgtacc tttcgcaaga
    17941 cagtggattc gtatctgatg ggtacgccct gtttttggac gcgcttcaag ccatgtccat
    18001 cgcgtaaacg actctatcac gctaaactct gtttctgaag gtttcccatg cgtacgatca
    18061 attttcatca atccgcgctc cgcttcaccg atcggcacat ccacgttgta ttgtttttcc
    18121 tgtacgcggc cggaaactaa tgccaaataa tttttttgga tggtatgttt ttcaaattgt
    18181 aaactgacgg attggtgcgc ttccttatgt ttagcgaaaa gtactacacc gctggtcggt
    18241 ttatccaggc gatgcacttt gaatacgaaa cccctttcat cacggagtaa atccgctaaa
    18301 ttcggcgctt tcgtatcaaa ccggtctgca atcgtcaaaa gcccggacgg tttatgtact
    18361 gcaatgaagt gttcgtcctc aaagagcaca acgatatccc taaatgacat actcatactt
    18421 tagcggaata ttaaaatagg tgagaagaaa aaatgatccg tttcttacca gcgttctcgc
    18481 gcagggcgac cttcgctaat atccgttgac atatcaatgt aacggctcaa tctaaacata
    18541 agttgacgtg ccgggccctt gaattgttcc acccgcgtca gagccaattc accctgcacc
    18601 gtttcatatt gatcgcggta tttgggtaaa accgtgtttt tataaaattc agggaagcgg
    18661 ctgttgacct cgataaactc acctttgtca taggtatata catcatacca ggaggcaatc
    18721 gcaccttggc tgaagtatga attcgtcgta tgaatcactt catcggtacc gtcaccgtta
    18781 aggtcccgtg catcgccgaa tgcgccaaag tctgcgccgc gaaatttgtc cccatcccaa
    18841 aaaataatca ccgtactgtt ttttccccaa gggttcccat gctcgacaaa acgcaaaaat
    18901 acatccggac caaatcggtt atccaaattg acaattttga tgtccgaact gtggaacgaa
    18961 ttttcggagt atttgcgttc aaaagcccaa acccattgat ccccttgttt ttttagaacg
    19021 cggaaaaagc atcgctcttt ggtatcttcc atattacgcg gcataccggc gataagtaat
    19081 tcgtccgtgc catccgcatc aaaatccaca taaaacccgc gcgtttcgcc tacgtacgct
    19141 acagccggcg aaggtgtctc ggatgtatca atgaggtgca ttcgcattaa ttcgccctgc
    19201 cggagggttt taaataaatg ccggtatgat tgggtcatat gttcatacac cgcgtccgac
    19261 ggaccggttg ataaaaattc gacaaaagaa gcatagatat cgccggcaaa ttgcacggga
    19321 aaataagtca aggataacgt ggaagcgtca caacgatctg cccacggcgc attgatctga
    19381 ctccggtcaa catcgttgcg caaaggtggt gcgatcatat agccggtcaa ccccgacttg
    19441 gtcaccaccc ggtgcggttc acgtatcgtg tcacacacgt aagtatgacg caagaccgaa
    19501 cgttcaatcg tatattcatc ggtcgggata ttgagaaggg acgactcctc ggccaacgct
    19561 tttactgaaa tatagcgacg cgtttccgca tcatatagtt caacgccctg tgcatcttca
    19621 cgaatactcc aggtcgaagg aatttcaact gaaaaaccga attttgtatt ggcataagtt
    19681 ttccactcgg ctggcttcgt attggtacga acaaaatcca ccggatggca taccaaaaaa
    19741 ttaaacacca ccaccatgcc gatcgctaaa acggccacgg aaagatggta cgtccattgt
    19801 tttgcgcgct gactcatata tacaaccaaa aggcaggtta tctgaaaact aaatcaccgt
    19861 tttcatctgt gctaaacatc cgaacagcgt cgcgtcaaag gtaggcgcat cgtgggcttt
    19921 gatcagcagg ttgtgaaagc gttcgctgcg cggttcggta aatgcgccgg ccaggacgga
    19981 tacgccacag gcacggagtg cccaggccat cgcccagtcg gccgtaaaag taaaatcaag
    20041 gatcatatcg tattcttttg cgcgaatacg atctacggct tctccgttta atgtccgtaa
    20101 caaaccaaaa tcggtcttgg ccaagatcgt tacggtcgct tgccgttcgg tcagaaatgc
    20161 ggaaacttct tgcggcgttt ccggatgtag caaaaaatcc aatgtacaac cggacagtgt
    20221 tttttgtatt tggccaaaaa tggtttcggc gcgtgattta tcgatggcca tcggaaaata
    20281 taccaacaca cgtttgatct cagataaacg ctgcggaaaa tccatcacct ccgtcggttt
    20341 gaggaaccgc gaacgccata aaaaccactg tgccgatata aattccatca tattctttta
    20401 caaaaacatt ctataaacga acatcaataa agaacttgga tttgattttt tcgagccatt
    20461 tgtccatttc tttggcctgc ttttgacgca gggcgaaatt ttcaatggtt tgccagtctt
    20521 cattaagatt caacttgcgt gtatcacgac ggtcatgcaa ctgtactatg aagaaaccaa
    20581 atggcgtttc aaacggttcg gagatttctt cgttttttag aggatccagc gcttttacaa
    20641 acgatttatc ctgcaacgtg gcgatctcta tccatcccat gttaccgaga ttgccttttt
    20701 tggccggatc gtccgaatac tgcagtgcgg ctttttcaaa agatattttg ccttccaaga
    20761 tatcctgacg aatgcggccc aatttttcac gcgtacgggt tttatcttcg ggaccattgg
    20821 ccacgcccaa taaaatgtgg cgcacgtgaa tgcgttcacc gcttttttca atcatctgta
    20881 tgatgtgata accaaattcc gatttgacga catcggaaat ttcacccggt tcaagacgca
    20941 cagccgcatc ggcaaattct tttacaaagt cgttgcgatt gaaccagccc aaatcaccgc
    21001 ctttttttcc ggaacccgga tcggtggaaa ccgccttggc gatcgttgcg aagtctgcac
    21061 cttttttgat cgagtcaaga tacatttgcg ccatgcgtcg cgcttcggca aacgcttcat
    21121 cgccggcttt gacctcaagt aaaatttggc tgaattttac cgatgctccg atctgaggca
    21181 agctgtcttg aaactcttca aagaattttt ctatttcgct gcgcgacacc ttgacatcgt
    21241 taaacttagt gctttggatg cgttgggcca gcatctgttt acgaatattc ggtcggtaca
    21301 agcgtttaag ttctttcacg gatttgccca tggcccgttc gagttcgtct tccccgccgg
    21361 cttgctggac gcgctcgcga atctgctgtt ccagtgattg ttcgacttgg gcttcatcta
    21421 tcgtgatact gtcaaaccgc gcctgtgcga gcatgatctt ttgctgaatc atggtctcta
    21481 atacgcgctt catgatttta tcaaattctt cggtattact cggattgatg tgtaggttca
    21541 tcgcttcgaa gtacgcatac tgttgcactt ccgacatcat gatcgcttca tcgtccacga
    21601 ccgcggcaat tttgtcaatt aactgacgcc ctgtctgcgc cgatagtgat ccggtcaaaa
    21661 taaccgacac ggccactgca ccccaaaaaa aacgacgaaa gaaaaactta ctgataatca
    21721 tgccattcct tatctctgta tctacattca atgtgtttat ttctcagtca atacaacctt
    21781 tgtaaaacgg acgaaacggt tgaatatcca tccgcaaaat atctttgatc actatggagt
    21841 cttggtcaaa acaggcacgc acttcttcca tattttctgt tttaaataca acaagaccaa
    21901 attcgccata ccgtgctcca agcgcgatct taccttccgc atgcaagcgt ttgagattgg
    21961 ctccgtgttc tttgaagtgc ggctgctcgt aggccgtttt ggtcgtatcc cagtttttcc
    22021 cgatggtaaa aacagccgct acgtaagact tcggcgcagc tttcggtgtt tccttttgcg
    22081 cccaacccga aacgacaaaa ctccacatca gaaccgaaat atataaaaac tcttttttca
    22141 attgagatcc acccatacgg atttgacctg cgtataaaat tcgtaaatcg cttccatacc
    22201 gcattcgcgc gtaaaaccgg acattttgta tccgccgtat ggcatcgccg catcgtacat
    22261 gttatatgta ttgatccata ccgtgccggc tttgattttt ttggccaatg tgtgcgcttt
    22321 cttgatatcg cgcgtccata tccctgcggc aagtccgtaa tacgtgcgat tggctttttc
    22381 gacgacatca tcaaaatctt tgaacttcat cgtcgcgaga accggtccga aaatttcttc
    22441 ggtagcaatt ttcatggacg tatccacatt tccgaaaacg gtgggttgga aaaagtatcc
    22501 gtcgttgccg atacgatttc ctccggcaag aagcgccgca ccttccgatt taccggcttg
    22561 cacataactc atcactttat tcatttgcag ttccgatgtg agcggaccga gtttggtctc
    22621 gggattcaac ggatcacccg gcatcatttt ggctgcacgg gcagctaatt tttctataaa
    22681 ttcatcatga atactttctt cgatcagaag acgggaaccg gccgtacaga cttcaccttt
    22741 gttgtaaaaa atagcggcgg cggccatctt gatcgtcgta tccagatccg tatctgcgaa
    22801 aacgatattc ggtgatttgc cgcccaactc cagcgaaaga cgtttcatcg tcaacgacgc
    22861 agttttcata atttcctgcc ccgtcgtcgt actcccggta aaggcaatgc cgtccacatc
    22921 ggcatgcgcg accagtgcgg caccggccgt ttttccaaat ccggttacga cattaaacac
    22981 gccttcggga aaacctgctt cggcgaacag ttcggccagc ttgagcgtcg tgagcggtgt
    23041 aaattccgac ggcttcacta cgaccgtatt gcccgccgcc aaagccgcag cgatcttgcg
    23101 tgctgccgtg atcaacggaa aattccacgg aatgatcaat cccaaaacac cgagcggctc
    23161 acgcagagta tagttaaaat aattaccgtt aacaggtatt gtttcgccct cgatcttggt
    23221 ggcagcgccc gcgtaatatg caaaaatatc tgccgttaca ggtatctcaa tgtttttagt
    23281 ttcggaaatt ggtttaccac aatccacagt ctcaaggtag gctaactcat cagcattttt
    23341 ggtaatcaat tcgctgattt tccacaacaa tttaccgcga cggcttccgc tcatgcgcga
    23401 ccacttgctt ccgtcagaaa aagctttgcg cgcggccgca acagccgtat caatatcgtt
    23461 tttatcgcct tcggaaatag cgcatatcgc tttacccgtt gcaggattga tcgtttcaaa
    23521 tgttcgattt gatgcggcat caacaaacgt attgtttatg aaaagtttag tgtttttgac
    23581 gtcaaaaatc ttaacatcgg cgggagcagg cattcgaact cctaaaaatt cagtgtttat
    23641 tttcgtaagc tattggatta caacgggata aagtacattg atcgaaaccg aaagtcaagt
    23701 tttttcacag tttatcaaca agaataagcg tatgtttgta tcattgtgaa taacattgtt
    23761 tataatctta ccaacgcttg tatatgttga gtttggacgg tacgcatatt atttcaacat
    23821 caaatttttg actgtttgca cttcattttt tttgcgaaaa aaaaaccgcg taattttttt
    23881 ggatacgcgg tttgaataaa tactatttat tttttctacg gcatggcctg atatcgtttt
    23941 aggttgtctt caaattcttt tcgcatacgt gagcgttcag gaatcatatt cacggcgagt
    24001 tgcccaaaac gtacggcatt tgctttatcg ccgacggctt cacaaagatt ggcgatggta
    24061 tcagctttgt aagcgtcctg atattcaagt acagtttcgt cccacgtttt gatcatcgca
    24121 gcggcaaact cgcgcatcat gattttgact tcaaacgccg tccagccgac gttattcata
    24181 tcatccagag aggcgttggt accgtacgac gacagccatg tttttagtac attttcggct
    24241 cgctcttttt ctttccattt tatgtgaatc tttaccgctt cagacacacc gttggcgtcg
    24301 ctcaaatcaa ttccgcgcag gcgcgtttcg tcttccgatt tgtccgtttg acccgaacgg
    24361 tatttgattt ccataaaatt ttccgcataa ctctgccgtt tttccgcatc cagtttagcg
    24421 tacagagcgc ttgctatttc ccatgctttt tctttctgat tgcggcgcgt aagataaaag
    24481 gcatagttaa acgccagcga aatcagatcg taatcatctt caaccttact tgtgcgaata
    24541 atttcgtccg cctctttagc ggcgctttcc aacccataat ccaaaccacg ataagcgtgc
    24601 tgctgtttta atgccgcact ttgttcgtcc gacatccctg gcatagccat aagttcggta
    24661 tagaacggtt cagccgatcg cggatcaacc gataaataat aacgcacaat tttttcaccg
    24721 agtgtcagat ttttatttgc attgtattcg gccatccaca ccggaagtac atctttggct
    24781 tcggcgatgc gcataagatc ggctatataa ccttcgatat cttcattgta acctactttg
    24841 cgttcgattt ctttcccgtc ggcgtacata aatacaacgg agggataacc ggtgatgttg
    24901 tattttttag ccagttcaat accttcgcct ttttccgcat ctattttata gcataccatt
    24961 ttttcggtaa cgggtaaaaa cgccgcgctg cgataaacat ttttttccaa aagcttgcat
    25021 ggtccgcacc acgccgtata aaaatcaatc atcaccactt gttttttttc acgtgccgcg
    25081 ttcaatactg acttgacatc accgtaaaca aatcccggta tcttatcgcc ggcatacaca
    25141 ttaaaaccta ccccaagcat caataacatg actagttttt tcatacgctc tccatgatat
    25201 ttattattaa tacgatcctt ttgtacgttg ttgaataata aaagttaaat actttttttt
    25261 catttcattc gtaccgtagc gcatcaatca cattggcctg cgaggctttg agcgcgggat
    25321 aaaatccgaa aaaaataccc acgagtacgg aaagcccgaa actcagaagt acggaatccg
    25381 gagtaacgac tgtgcgccat cctgcattcc actgcagaac atacgccgag ccgataccaa
    25441 taccgacacc gatcaatccg ccaaacaaac aaatcaccat cgcttcgata agaaattgca
    25501 ggagaatatc cttgcgtttg gcgccgatcg cttttcgaat accgatttct ttggtgcgct
    25561 ccgtgacgga tacaaccata atattcatga taccgatacc gccgacgatg agcgaaatcg
    25621 ctgcaatcga tgctaaaagt atcgccatcg tatcgcttgt ctcttgcgcc gtcgccaaaa
    25681 tatccgcttg attgcgaatg ctgaagtcat tttccgcatc accgcgcagt ttgtgggttt
    25741 tgcgcagagc gcgttcaata tcaaacgtcg tctcatccag catgttttcg tcatatacct
    25801 gcaccgctat cgtcgtaaca aagtccaatc cgaaaagccg cgtctgtgct gtcgtcaggg
    25861 gaaccatgat ctgatcgtcc tgattctgcc ccataccggt ctgtccttta ccggcaagca
    25921 caccgatcac ggtaaaattt tggccgttga ttttgataat actccccacc gattgggcac
    25981 ttccaaaaag tgtttcttcc acggtctggc ccaatacagc aactttgacg tttcctttga
    26041 tttcttcggc ggagaaataa cgaccttcag ccaaaacaaa atttcgcact ttcaaaaatt
    26101 cgatgcgcgt accggtgatc gttgtattgg tattcttaga ttcatatttg acttgcgcat
    26161 tacgggacaa ttccggagaa acagccgaca catgttccga agttttcaat atggtctccg
    26221 catcttcctc cgtcaatgtt tgcgaactgc ccgccgcaag caccgtacgg ccgacatttt
    26281 gagaacccgg tcgtaccaat agctgattgg taccgagccc ttgaagtctc tcctgaatcc
    26341 ggcggcgaga cccttcaccg atcgccacca ttgtgataac agcccccaca ccgataatga
    26401 tgccgagcat cgtcaaaaac gtacgcaatc gattggacgt caaagcccaa agcgcgctgc
    26461 gaagactctc gagaatattc atgatgaatg atagggtaat gttactacaa aacgttcaaa
    26521 ctcagcgcga aaatgaagtg cgctctttca tgcgatcttt aaatcgttcg ctttctttaa
    26581 gcgcttgtga atagacgacc aacatgacgc gcgacgaatc gtccaatccg gacaacactt
    26641 ccgaaacatc cgcattggat aaaccgatgc gcaccggatg cgcacgcaat gttccggctt
    26701 cggaaagtac gacaaaacgc agacgcgtcg aatcactgcc ggaattttgt tcacgcatac
    26761 gcttacggcc cataccgcgc ccgctggagt cttgcgtgcg atcgcctcgc ttttccggta
    26821 ataccactcc ggcttcttct aaaatttttt tatccgcctg cacctccgcg tacgtacgca
    26881 gcaagtcgtt gcctatcaac aaagcatggt ctttccctgc gatatctacg gtcacggtcg
    26941 cattcatacc ggctttgagt ttgtttccgc gattgggtac gagcaatatc acatcaaagg
    27001 ttgtaacgtt ttgaatgacc gtagattgtg ccgccacacg aaccacttca ccgaaaaaaa
    27061 catcgccggg ataagcatcc gccacgatgc gcccgcgctg acccacgcga attttcccaa
    27121 tatctacttc gtcaacagcg gccgtaagat acacgcgatc catcgccgcg agccgggcaa
    27181 tcgtcgttcc tccgcccgca ttggatacag ccgaagccac gatctgacct tccgatacgg
    27241 tacgcgacag gacaatgccg tcaatcggtg aacgtacggt cgtttctgcc attttttggc
    27301 gggctaaaac aagattggat tgggatttga ccaatgtggc tttggcgcgt acatactcga
    27361 cattgatctg atcgtattcg gattgggcaa gcaaattttt ctcaaacaaa tctttagcgc
    27421 ggcgccagtt attttcctgc tgtatcagca cggccgaagc aacttgcgag tcggcaatag
    27481 cttgttccaa cgcattgcgg gtatccgtct gatcaagccg agcgatcaat tgaccggaat
    27541 gcacgacatc gctcgcttcg atgggaattt tttccaccaa tccgctggct ttggatttaa
    27601 cttccaccac attgatcgga ttgataacgc cggtcgccga cacatccaca ttcagtcgtc
    27661 cacgctccaa cgcggcagga cggataacca acgcgcttcc cgatgcgttg cgcttagttt
    27721 ggtcgctacc gttacccttc cacaggtaga gcgcgccgac acacaagcat aaaacgatag
    27781 atcctaaaat atattttttc atctcaactt ttttcccgct cgataaagag atatttttaa
    27841 aaaaagaaat tatttttttg cgggatgcaa atcgttttca tgaatagaaa acgaccagcg
    27901 ttttttattg tgttcatccc aggtttccat agtcattaca cggtctctac gcttccccga
    27961 aaaacgtata acgccaaaag tatgcgcatc cgttattttg gtgccgtcca ccaattgcgg
    28021 attggttcct accgtatcgc cggaactgat gccgctggtc agcggcgatg acgtaaaatc
    28081 gtacagcgta taaaaatcgg gatcttccca tttaagaagt tcgctgtgat gaatgtcacc
    28141 gctcaaaaat acgacgcctg tgatgccgtt atctttgata aatttcagaa gatcggcacg
    28201 ctcattttta taaaaattat aactctcata cgtcgtgttc gcattgagca cctgattacc
    28261 gcaaacaatc accttgaacg gtgcattgct aaaaagcaaa ccgtctttga gccactgaag
    28321 ttgtgttttg ccgagcatgg ttttgtccgg accatccatc atatcatccg gtgcacgatg
    28381 ataccggtca tccagcatcc aaaaatcaat atcctgccat tcaaattgaa aaaaaacgcc
    28441 cggcgattca gataatccgt agtttggatt ggcccaatac agcttaaatg cgtcgagcgt
    28501 atggtctttg tgagcataac tgcgatcgga attattcgga ccaaaatcat ggtcgtccca
    28561 aatagcatag ttatgcgtat ttccaagcaa tggttgcagt tctttgattg cgcgggtatg
    28621 gcgattgcgg tagagaattc cgctgcgtga attccagtcc acttcgcgca aatagacgtt
    28681 atcgccgatc cataacataa gatcaggttt cacattaccg attacgcgca taatttcgga
    28741 tgtattgcca taagcacggc ccgggcgatc atacggcgga tcattcacat aaaggcatga
    28801 accaaacgcc agcgtgaagt ccggcgcatc ggtccgccac tcaaataaag cctgcgtttg
    28861 aaatgaattg gcatacggca aggtcggtgt ttttccgtca agcacgattt cataaccata
    28921 acgcgtaccc ggctctaagg cttcgagcac gatatgggcc gtaaaatcgg attctttcgc
    28981 agtacgaatt ggttccgaaa tctttgaaat tttcttttgg tcctgcgttt ttactgcttc
    29041 taaactccaa tatcgaatgc gcacttccgc agattttttg gtctgtaccc aaatcatcac
    29101 ttcccgtttt tccgcataac cgagcatagg accggcttgc aataaatctt cacctccggc
    29161 aaccccgtta accacaccga ccatccagag catcgcgctt aaccgtaaaa accaagacat
    29221 aaaaaagaaa cctttcagaa gattatttga ttcggctcta atataaacgc ctgacctaat
    29281 gaagcatagt gttttttaaa aatttcaaaa tggccgaccg atcataatat atacgatgcc
    29341 ctcctgatgc ggcgcacgcg catacgcaag ataaagcgga cctacgaccg tatctatgcc
    29401 aaccactacg gctcccgaaa atttaagatc acgcgatgtg atttgcgaat gattatccca
    29461 cgcgtttccg gtttcaacag aaaatccggc atacagacct tcggtgtaag atgcattcgg
    29521 cgctgatagt cggcggtaat acatacaacg cgtcattcca aaatgacgtc ctttgatctg
    29581 tcccggtcgg tacccgctca tcctcaaaaa accaccgagt tcggacaatt catatatggg
    29641 actggatgtc ccgagatcgg atccggcttc aaacgctccc attatcgaat tacgatccca
    29701 tgtatgcacg accatcccgg caccgcgcaa agtctgataa gcgccatccg atccccattt
    29761 tttttgtgat gaaaaatacg cgctctgcca gatgctccct tttttgggaa aatccggatg
    29821 atcggtcata tcaatttgtg tgcggatacg aattcctgag cgttcggcat ttatggattt
    29881 tcgtgatgcg tttgacgtgc tgtgggcatc aatgtgaccc caaattaccc cggcacgcag
    29941 ttcgccggcg ttcccaaatg ctattccaaa gtcatgaccg gcttcggccg catatccgtt
    30001 gtactgcgct atctcgacgc gctgctgata aaatccggat tcaaaccgtt gagcacgccc
    30061 atacggcgcc ataaaaaaat ttcctttgaa atctaacggt tgataccatt ccgtaaagta
    30121 ggtagggttt tgcccgacac gaaaaagatg tttccattcg gcgccgagtt gattgcgtcg
    30181 cgtcatcgta tagtccgcca acgcattaac agacgaatga ccctccaaat ccgtagccat
    30241 cgtgaggcca aatcgcagat agttggggcc ccaagacttc catcgcccat caatcaaaag
    30301 tccgcgactc gtatcttcgg tcactatatc aaaatccact ttttcaaaat caccgagcgc
    30361 atagatacgc gtcagatcgg ctttgagcgt tttcatgttc agcgaagaac cgggctgcgt
    30421 tttaagacga tgataaatcc gggggtcttg tccgatattt ttaccgatgc ggataaaatc
    30481 aatacgatcg tgtgttttgc ggcgcgtgcg aatttttttc ctaaactcgt tgtagatttc
    30541 atcgctcacc gaaaaacgac gcaacatcgg aatccactgc cgcgcggctt tttcgccgtc
    30601 ggtaataatg gcatccgcct gcaaataatc ggcgacggaa actgaaccga tatccggtcg
    30661 tattaatacg tctttattcg taagtaatgc ggcttgctgg ttcacatttt ccgtagtcac
    30721 catattcatc acctgcgccg tgatccccag taccgaattc aaatgcgagc gatccatcag
    30781 tggcgctccg atatctactg ctataattac atccgcgccc atcgcctgtg ccacatcaat
    30841 aggcaaattc tgaacaaaca taccgtctac taataatctt ccattgatct ccaccggcgc
    30901 taaaacaccg gggatagaca tgctggcgcg catggcttcg gctaaattac cgcctcgaag
    30961 gactacacgt tcaccggtgg caatgtccgt agcaaccgcc cgatacggca caggcaaggc
    31021 atcgaaatcc tgaataccgc tgaccggtag agtgattgtt ttgagcgtaa aacttaattt
    31081 ttgaccggca atcacacctt gcggaaatac gacgttccat gaacgaaacc ccacttcgaa
    31141 gcccatgagg ttttcattct cctgtgtttt ttttctaaat gaacggaagt cacgggacgg
    31201 tttgtcattt aacatatctt tccaatctat gtcgcgcgca aatttttcga tctctgcgac
    31261 atccataccc gacgcataca tcccgccaac gatagcgccg gcactggttc ctgcaatcac
    31321 atccacgggt atacgcaatt catccaaaat ttttaaaaca ccgatatgcg ccataccgcg
    31381 cgccccgccg ccgctgagga ccaagccgat acgaggacgg tacgtagtat ttagggtatc
    31441 agcaggcata cccatcaaga aaggagcggc caaaacaccc gcgataacaa tgcataaagt
    31501 ttttttcaat aggaaaatca tcgcttaaat tttactccca atgtaaacag gcgatcgtga
    31561 aatgaccaac gcggttcatt cgggtccata tctgtcaaac ccagagaaaa acggaaagcc
    31621 acatctacac ttaacttagg cgaaatggcg taatctatag ccgcgcctaa ctctccggcc
    31681 gcactccaac gattggcata atcggtttga ccttcgaaat ccgtagcatt cccatttatt
    31741 tcgatatagt gtgttttaac cgcataaccg acttttacgc caaaataaaa tatcggcgat
    31801 acgccccaat agtccgaact agactgcaac accatcagca ggggcgcttc gatgaccgtc
    31861 gctgaccatc gcgcgtagct gctatccgtt cgcgcatccg ttttttttcg aatccaaagc
    31921 aattcacccc gatatccgat ccatgaaaca aaattacgtt ccacgaataa cccgacgacc
    31981 ggcctcgcgt aaccctgagt cgtagtaccg cgatacgaat gacgcgcatc ggaaaaatta
    32041 acgccgctaa aaaaaccggt cgaccatgac tgtgcgttca aaggagtact cagcgtcatc
    32101 atacatacga gaggaaatat ggtattgatt ttgtacatcg gagtgggaga aataattatg
    32161 ctacacgaat gtaatcatgc ctatacatat ctacaaacaa aaactccctg cctttctaaa
    32221 ggcagggagt tttgaaaaaa ttaatttact atcaacttat attgtcagct tggcaatgtt
    32281 atccttgaca tcttgtgcag atttattcag ttcagcttgt tcgtccgctg tcagtttgat
    32341 ctgtacgatc tgctccatac ctttgcttcc gatcttgatg ggcacgccgc agaatacatc
    32401 tttctggcca tattcgcctt gcagccatac ggcacacgga agaatgcgtt tttgatcttt
    32461 gacgatggat tcgatcattt ctacagccga agatgaaggg gcataataag ccgaaccggt
    32521 tccgagcagc ttgacgattt cggcgccgcc atcgcgcgtg cgttggacaa ggcgatcaat
    32581 ggtagcttta tccatcaaat ccggcagagg aataccggca accgtcgaat aacgaggtaa
    32641 cggaaccatc gtatccccgt ggccgccaag tacaaatgcg ctgatatcgc ggacggacac
    32701 attgagttcc atcgcgataa atgaacggaa acgtgcgctg tccagcacac cggccatacc
    32761 gatgacacgc tcacgcggaa aaccgctaac tttccaggct acatacgtca ttacatccaa
    32821 aggattggtt accatgatga tgatcgcatt cggggaacgt ttggcaattt gttctgtaat
    32881 gcctttgatg atttcggcat ttttcatcag cagatcgtcg cgactcatgc cgggcttacg
    32941 ggccaaaccg gcggtgatca atacgatgtc ggaaccggcc gtttcatcat aaccttgcgt
    33001 acccaccata cgcacatcaa aacgctcgac cggggccgat tcgtacatat ccaacgattt
    33061 accttggggc atacccgcta cgacgtccac gagtacaact tcattggcca attctttttc
    33121 ggcgatccgt tgggcagctg tcgcaccgac attgcctccg ccgacgacgg taattttcat
    33181 atacaatctc ctgaaatata aatgttaaaa gatgaacgca cgtatgatac acaattcatc
    33241 caagaaaaca aaaaaaaacg gacagcgtgt ccgaagacga cgttgtccgt tatacagctc
    33301 tgcacaggta ccgcggctta agctttggct tcctgcggat agacgctcac gcgtaaacgc
    33361 gttttgtctt tgtgttcaaa cttcacgacg ccgtcaatga gcgcaaacag cgtatcatcg
    33421 cttccaatac cgacattgcg gccgggatgg aatttggtac cacgctggcg aaccagaatg
    33481 ttaccggcga gaaccgtttg tccggcgtag cgcttcacac cgaggcgttg gccatggctg
    33541 tcgcggccgt ttttcgtact tccaagacct tttttgtgtg ccatggaact ctccttactt
    33601 cgtaatagcg gttacttcga cttccgtgaa gggctggcga tggcctttct ttttctggaa
    33661 gcctttgcgg cgatacttgc ggtaatggat gactttgtca cctttgccgt gcgcaatcac
    33721 cgtcgtttcg acttttgcac cttttacggt aggctgaccg acttgggccg cgccattttc
    33781 ggagattaaa agaacgtttt caaaaacgat cttgtcgcca acctccgctt cagctacgcg
    33841 cggaatgacc agcgatttgc cggcttcgca cttatactga aaacctttga tatcaacgac
    33901 tgcgtacact gtgtcctcct ggacgttagt taattaggga cgccaatata ggcgacgaac
    33961 tataggaacg caaggatttt tttatcaaaa atagataggc ttttttgagc gcttttactc
    34021 ctcgctaaga ggagttacca cacaaccgta tgcagttttc tgtcatagtt catcggcatt
    34081 cgttcgacgc ggaacgtctg cgaaccgagc aacagaccct cctccgtagt tatttcgacg
    34141 cgccatgccc cgggggaaat cacacccttt cgagagtatc cacgatatcc ggcgtcgcgc
    34201 ccgccttgca aagtatgact ctgatgatcc tgcaataccc acgcgttacg atcgtctaaa
    34261 tattgctccc actggtaatg tatcgtggtg tgaatttcgg ttggggcaaa aatcgcggca
    34321 aaacaaatta ccgtgtcacc gggttgccaa tgatacacat cccccgatgt gcgccaaaac
    34381 tcccatgcct ccgctttttc atattgcaat tcataccgtt ggtccttttt tttcatcgtg
    34441 tgataaatac ccacatgacg cagtgcaagc ggaaccggcg gaatccaccg ggcaaagtag
    34501 aatatattca taaccccata gatcacgatc atcagtccgg cgcttttgat caagagtttg
    34561 cgatatcgcg ccctcgctat aagcgcaacg gcaaaaatta gcatcatggt aaacactaaa
    34621 ctgatgcatc ctccgatcaa aaaggaccga taacctaatt cacgagtaaa cagcggaaca
    34681 aaaaaaataa aaaaagagaa tgccgccaaa aaaaacatca caaactgaag ataaaactga
    34741 tggatgcgct ctttataaaa ctcattggct aacaatagaa ccataagtcc ggctataaag
    34801 accaatgatt tgaatccggc ggcactttga aaataaaaat aaacatggtt gctgtagaga
    34861 ttgcccaaac aaaaatgaaa acccaaggga agaagctttt gatacgtcac ccaaaaaccc
    34921 gttttgattt tttcagcttt tacggcgtgc aataacagca atagaatacc cgcaatgaac
    34981 aaataaccca ttagcaggta attttgatcc attgcatcaa tgcgtttgga agaaaaagca
    35041 tcccatacga atccgagtaa aaacgaaacc ggagaaatca cgttttggta tcgcgtatac
    35101 caccgggttg ttttttttac ataggttgaa actgatttca taagcgtgcg atagtatttg
    35161 ttttttttat caaatccgct atcttcagcg cgtttggttt atatatcaac aacaattcgg
    35221 agtatgatct atggcacgca tggtgcactg tataaaactc aatcaggaac tcgaaggtct
    35281 cgataaagcg ccgatcccgg gtgaaatcgg caaacgggta tatgagcagg tgtctaaaca
    35341 agcatggaaa atgtttgaag aacatttcaa aatggtaatg aacgaatacc gcctcaatct
    35401 gatggatccg cgtacggatg aaatattcaa acaacaagtt cagcagtttc tttttagcgg
    35461 tacagcagcc cgcccggaag gatttgtcga acctacatcg taaatcgtat cagggttcga
    35521 tctttatttt tttcgatttg atgcccttaa acatgaggta acatccgaca ataccggtta
    35581 taataccggt ccagaaaaga acaacggcat aggtacggta gttactgata taattgaatg
    35641 cgatcagtat gcccgcgagc gacaaaggca taccgaaata aaaacgcttc gtaaaacgta
    35701 tcataattcc accgtcgtat tacgtttgat ttttttaata tttgaaacag cctcactgat
    35761 ggacttgggt cgcactgaca gataccgctg cgtatagcag gtagtccaac aaccttcgca
    35821 ccggctgtat tcatctaatc tttgtttgta ttcataactt tcgaaaattt tctctaacga
    35881 ttgccgaaaa acatttccta tcggattacc cttgcagaga tgcacatcgc cgttttccat
    35941 gatcgtcgtg cgtgtcgcta agtacggtgc ttttacaaac gggcaccaat ttaaattcgg
    36001 atcttccaca taaggaatga cgccgtcaat ccaagcatcc aaattcatca cgtcgggatt
    36061 ttgctttaaa aacgtcatgg ttttatttaa tcggtcgccg ggacgcaaaa ccatatcatc
    36121 attgtaacgt gtcgaaaaag tcaacgtatg gtacattccg actgttgatt tccatcctaa
    36181 agatttgacg tgcgcgatga tatcggggac ctgatctaaa ttgatatcac tcatcaccgt
    36241 attggcataa agtacagatt tctttttatt tttcttttgg atatctcgtc tcagctcact
    36301 tattctgcgc atgttttcgg aaatcactgt ttcgaaatct ttgcacccgc gcaaatgatc
    36361 cccaacctcc cccaaaccgt caaacgatgt ctgaatatgc attccgtttt ccatacaaac
    36421 ccgtgcggcc ggctctatat tttcccattt attgtaaagc cccgtcacaa gtgtggtcgc
    36481 aaaacgaaaa gttttatgcg cttcaagcaa catctccgac aaaccatcca cgatcgtcgt
    36541 ctccccgccc gatatgaagc cgacatacgc tccgtaactg cgcagatttt ccatgacgtg
    36601 cgcaaattcg tccaccgtca tcgtatgagt atttttgaaa ggaatcacac actgccgaca
    36661 acgctgcgta cacttgtagg ttatccgaac ttcggtgctg gagttaacag ggcgccggtg
    36721 ggtgatatta ttaagtattt taaacattct ttattttggc atcaaaatgg atgcactcgc
    36781 tttattttat ggctgcatcg ttatagacga catgcgttcc atactgcaag ttagtcggat
    36841 caattgtaat tcttataatt atttttaata aaaaacaaat tcattttttc accatcgatc
    36901 cattctttat taaaaaagct gatattgtat aaatacttcg atttccaata tcaaccaatg
    36961 ctaaatattc tcgcttatga agtcaaaact accaaaacag tattaaataa taccgttttg
    37021 gtagttaggg tatcgaactt aagaatattt aagatatgaa taaccagtat ttattcagca
    37081 ttttttttat aaaaccaaga ttccgttgca aagaacatcg ttgtataaaa aaaatgcaat
    37141 ttaaccgtga agcaatctgt ctaacgagta atcacctccg ccgctgatca gcaatgccgc
    37201 acaaattccg atcaccagta aatggtattc aaacccctcc ccctgctgat taccaaacca
    37261 attcataaag aaaccgtgat gatagttagt tgttacgata gcgcctaaca ttataacaat
    37321 aaaactcatc gcacatactc tggtgccaaa accgagaata agccctatag caccaaaaaa
    37381 ttcaattaga ataactagta ctgcgatcag ccaaggcaat ctcatggttt cagtaaaaaa
    37441 attaactgtg actgaaaaac catacccacc aaaccaacca aacatttttt gagccccgtg
    37501 aggtagcatt acgaagccca ttgtgactct caaaataaga agcaaccaat tattgtccgc
    37561 aaaaaaaaga aaagatttca tgacagcacc tttttgatgt gataaaataa gatttatttt
    37621 cacacctata gtattcaatg ctgttacaca aaatcattga actagtttaa gaaatcagta
    37681 tttttttgcg aatcatactc agaaattcag atgtaatacc taaataagcc gcgatgagtt
    37741 tttgaggaat acgctgttca agtgtgggat acttttcttt aaaacgtcgg tatcgttctt
    37801 cagccgtcat tgattgatcg tgcataattc ggtgctgaag tgcaataaag ccattttgcc
    37861 atcgttcgtt tgaaaattta tgaaatgcag ggatttttct cagaagcttg tcgtaattac
    37921 ttctactgag ttgaagtact ttggagtctt caatagcttg aatataaaaa aaagaaggcg
    37981 tttgattgat aaaactttcc agatcaacga cccaccagtt ttctaaagca aactttacga
    38041 taaattcagt tcctgtatcg tcactataac ataccttaaa acaacc
//
LOCUS       DAOQGF010000019        37496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_474_length_37496_cov_7.322774, whole genome shotgun sequence.
ACCESSION   DAOQGF010000019 DAOQGF010000000
VERSION     DAOQGF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 37496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_37496_cov_7.322774"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK937_04615"
     CDS             <1..779
                     /locus_tag="PK937_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNH31883.1"
                     /translation="NFRVAPPMWGRGFIQEIPAETIVANADPNDANDDGISGRANYIV
                     MSFVDSPTHPELGRHGLKAQMSRILDFADNAFLHDLGMTSPMRPYELVNPNARDAKDK
                     KSGVDISHEDVEKVDFFIRTSDFPPILQETEQIQIGKNLFNEIGCNKCHIPSMTTGTS
                     EISALSNKTVFFYSDFLLHDMGQTLSDGVLDGDAKPQEFKTPTLRGLRFFKDFLHDGR
                     AKNVDAAIRMHGGEATTVRNNFVGLSQEERDAIIAFLNSI"
     gene            804..1847
                     /locus_tag="PK937_04620"
     CDS             804..1847
                     /locus_tag="PK937_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31884.1"
                     /translation="MNRSKSLLIILIAALVLIPSCDNVESGTFSGIYFGRPPRKLRDG
                     QGYFEMWLGFPASSGGKEWVSILKFDRVESTNKPFLNDKAAILKRPNRNINDAVSAAI
                     SIELENDIDPSVPNRIFMSGEFTDGIASLELSGEDALNLDLSDPAVISGKFVLGTPSD
                     AINNNDDSGVWFVDAPFGQQQNPGLSLPVLPIGFKYEAWIATQSETVCISTGQFKRAD
                     SLDYNGKGPNGGGELTPNVPGEDLVFNQFNEDVQGRTVDASLQEAYLDTFGFPIHIKK
                     TIQKNPLTNAKDYWDVVVSVEPDPDNDPEEPFAIFPLVKFGPIFESVNTVIPLTNFTG
                     GVSQYIVIDLSKM"
     gene            2427..2591
                     /locus_tag="PK937_04625"
                     /pseudo
     CDS             2427..2591
                     /locus_tag="PK937_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2839..3726
                     /locus_tag="PK937_04630"
     CDS             2839..3726
                     /locus_tag="PK937_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH31885.1"
                     /translation="MFWNWFLAIGLFVSLSNILFSQAQDSIRIRTVPVAGKVWMMDCE
                     NGFGGGNVAAMAGDEGVLLVDAMYASMTEKIKKAAFTLSNLPIRWVVNSHFHRDHIEG
                     NKNLRTSAVIIGQENIAKRLRSKHTQTTPTLDMMPSVFFSDSLTLTVNGEDIRIIHMP
                     GSHTSADVVVYFTQSNVLHLGDLFFHGMFPAVYTDGGGNIHQLIASLDKIEKMFPVDV
                     KIIPGHGPLATMPALHEYIIMLKETVTAAQEGLRNKQTSDQLLKTSVFQKYNYLGDGG
                     AQTTEQYINMLFKLLSESK"
     gene            3770..7780
                     /locus_tag="PK937_04635"
     CDS             3770..7780
                     /locus_tag="PK937_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH31886.1"
                     /translation="MNYMTVVMSRTLCQARVFLWMFIVLCIQSVGMYSSYAQQSFLVT
                     NGTDGPNPGPEGSLRRAVYEANTWPGQDIIIINCSDILLTSGSLIVTEGVRVSSGELP
                     SEHLFNNWGLHADDPLNDTLWTIQAGQMVEIIGLNMFNAKIGVYIASNGCFITYNKIS
                     NCRTGIYIKGQNNGILNNAFGFAKPYPYSNYAAIEPNGTAIADHSSGFNTFLGNYFAA
                     SSETELYIGATAREDGVYGNYFGFNNPSIQSDTVFSYKALTIYGQATNVGGKRDGSYI
                     NPNVFASYMSYGEVYSDSNVIMGNMFGLQHDGQTPAPFLAAGLTIYGDDNLIGHHTRP
                     GRNVFGSGENASALLLIQAHRNTIIGNYFGTDSTGLSAGLLNRGIQLVSGSSENIIGG
                     ATVGERNIFSTTQVDIYVQDGTANHILGNYFGLNADGSATLVRGGIGVDNASPGLIVG
                     DGSDDGRNYFVNKSTAINTYTSSRATEVQNNVFGLMPDGVTVEPNNTALVLSGNASVI
                     DDNIFAGTNAGRAIQVFADSCFLSGNIIGVDVLGNGPKGFNIALEINGENNRIGSRTS
                     GEGNSISWSTQLISIGSGANQNHIEGNAIGLGIENQIRGGGHGISVNQARGNTIAYNK
                     IGSIVNAINLSNTYNTHIHHNAIGVADSLGSIQRGNSGVGISITSNTSGTLIEHNTIA
                     YNNISGISIVDAESDSNRWYWNRIYENKSGITRPSNSKNSVTPPHIMGGIANVGVWGK
                     AQPGSRVELFLDEPGKIQASDIIGGTYADAQGTWAISVPGLSEGQTLAATQDSAFNSS
                     LWSTRYIPSSAGNILERDHGYRAVISLGSIKAFDSISVPLRFYADDGSVISQIAMHSG
                     DEFSWDGKNADTLDHSYPDTLIGWVKFKPESEGVFSDTLRVVYDSRVFALTLEGVATE
                     PSVLTSEELQYSFPNTRVTYVSNRQVELYAPKAPLNVTAIRLARAGTSGYSFDGSSLP
                     YSIDSDTLRVGVHFTPSAIGVSLDTLIFSHDGYQRELRIPLQGLGLENQAPVVHTGLL
                     RSTVLKDRIEFYAVSDEPLSSVSLKINNTTVPLQIVPAHTRMYRAEYEITADGELAFE
                     VTANDTILNQAIALKTYVIADLGSGSTSVLRSGQAEMRILKSSARAEGYLILSEISSD
                     NDLTNNGSNILQTCEILSTASQSWSSELSFNIAPAHRSDWNLTIPGFSTKHLKITRLD
                     ATGGWERRMIAHADENRVYTSINESGRYALVYDPSFSEIPKSLDLAQNYPNPFNPSTS
                     IRFGLANDGYVRIAVYNVLGQKIRNLISEQRTAGYHNVMWDGKNQKGSAVPSGIYIYR
                     LETVDGILSKKMVLLK"
     gene            7800..8357
                     /locus_tag="PK937_04640"
     CDS             7800..8357
                     /locus_tag="PK937_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31887.1"
                     /translation="MKHIKHISKPILFLSLILTCATGACDDYEASLLDAAWKKFENQD
                     YDGAYAEFSALNGPNARIGLGWTLLRMDSIPEADRAFRIAAADSVLDGYAGIAITSWE
                     TGRYASAIYAANFVERWDPEYYFVHDPSVELNDLILHKAFCQYYLNDLTACNASIKKL
                     DPAWVTSTNPATILNKLEVLYDQMD"
     gene            8456..10597
                     /locus_tag="PK937_04645"
     CDS             8456..10597
                     /locus_tag="PK937_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31888.1"
                     /translation="MYKVLLLILVMVPLTLFGQIPDGKKILDKAKKKNKEESKKEVKD
                     KSKKSDDPSKSTATEKKAGEIPRIQQGSTAAKENSESKKSTHDDYQKLTVSADSDPFE
                     VKTLKTYIVRMLECLTEVETDNSKQDQNIADHVFSKFEIHHSRAEESLTTVRTGKLLA
                     NADVYESAMREIKQDFEEAKKTFNEKSRFISVQSKIRRMVSDVQDQVASLKQRSNYDS
                     YMTSQAKGDLDEARTALQNAIKEWPQLSTSSYAADLLQYEKELQAIESSGNASEGFKK
                     LIDKAWFEGKFLLQNGLQYGSFTKDAAQSFYENCAKINYPKALSALNSAKPNNDVNSS
                     QIEYLTKEFPTEFNAYLKKGYIADINSHIEKAYAYKANGKTQLSLALEEAETAVIMAD
                     AVLLVKPDDVSIQRLRKDGLSTVEKMKTEIDAAEATAFTSVFHKENKSKIVFSKNRIS
                     LKQENASTSSDFFNGGEPIYAMAYFTAMVKDLPKMYRTANPVVRIKYYVDVPADDAYA
                     VPTATTEYQIMASNKTLMEATTLEVEVAPNAGQTDRPEVGAQAAKMLSQLSPRLHSIR
                     VVLSIDNENFATGSFELDGSQGLEKFAALGDDLIKSKIQNARPPKGGGLEKTIEQGIR
                     SALKSEGFNVLRVIQTDPQWYYERHPISGAILNRTIQAAIIYKDEQNKCIVEYMEFTQ
                     KYINSQYDALYRSMRGTVTKPYEVLPEHVNE"
     gene            10658..11734
                     /locus_tag="PK937_04650"
     CDS             10658..11734
                     /locus_tag="PK937_04650"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31889.1"
                     /translation="MIKILLSKWPRIHWHVFLLGFAILSCDSKETNEDKVEPVNPYTD
                     LRWRVSLTKVAKAITSNPVNPNTIYVNTYSDYVGRGGIFISYDRGETWPAAMSPTIDT
                     STNQGAGDVQFIAVCPNDTHIVFAGGVHSSGLLRTSNGGKNWVNVLPGVDADAEAMAF
                     NPQNPAQVFFMSYTDAGFYVSQDTGRTWQLRKFTGFGYSCTLRMHPSDPNIFVAGASK
                     GKIIKTKDQGYSWYLVQKQVSNQPNEWPEVPAIIWDNENPNKLYATISGANGKSFSQS
                     NNGGETWHIKDLKEELWAAHMTKSKLVIFGGWQSTYITSDQGKNVFKIGGDETVSGYW
                     SITSSGNRILAISSYDRSLMMVDL"
     gene            11780..12241
                     /locus_tag="PK937_04655"
     CDS             11780..12241
                     /locus_tag="PK937_04655"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNH31890.1"
                     /translation="MKLLIRGILIMLLVSGCSKNEQQENADKFGEGITLKKVVRMPEV
                     LNNPETYDGQEIVVESTIKTVCQARGCWMEVESGILPMRISFKNDSYFIPKDAARRKV
                     KMQGVLKVKEVSEEVARNYLKEAGRSDEEIGKINGAQRLLTMVASGLAIYK"
     gene            12330..13082
                     /locus_tag="PK937_04660"
     CDS             12330..13082
                     /locus_tag="PK937_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31891.1"
                     /translation="MKYILVIALLVSLGRQSLAQTETIEHGVGFYVKGGWFFDRSSGS
                     EDHSPELKGLKGELGNDISDRSVYIGVRYKKIEAEMGWGEIIKNSDITIYPVESRAAR
                     YWAGSVAYYVPFQIGSSNPIKSKLALGVKYSTYNTSYVDESITKLSIDKWNIKSTWFP
                     ERSYFSFLFPTVSFFYENQQKLANQSEFGPAHSIGIALKPAAVFPLGILPVKIVIEPG
                     MSWSHKLSGSDHFQKVYDTVYSLDAAIVYNLK"
     gene            13124..13528
                     /locus_tag="PK937_04665"
     CDS             13124..13528
                     /locus_tag="PK937_04665"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNH31892.1"
                     /translation="MGELNFLAIAVAGFAYFMLGALWFSPFVAGTYYDKALGFTRTKD
                     MKWGVIYYIGPFMGCVISTFATAVLMRWCSVISMGEATKLGLIVGMGYGMVISITNAI
                     NPNTPRPLMYGLATGGYHVIGNIMVSVLIYIM"
     gene            13709..14497
                     /locus_tag="PK937_04670"
     CDS             13709..14497
                     /locus_tag="PK937_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH31893.1"
                     /translation="MSADYNRFSEKYGDHLSQEGDLYIPIGQPKAIVCFFHGGFWRAP
                     YGRDQFDSVAIDFAQAGYIIWNIEYRRVGEVGGGWPGTFEDAILSVNYLSTLQKKYRW
                     SDNLNVVLAGHSAGGQLALWLTAHAEKKFLQHTPLTIHPDLCIGLAPVVNFEKAYQEN
                     LGGQAIYKLLGCTPKENPERYTCYDPLFAVHLNTPQIILHGEMDEDLPIAYSISYLRA
                     AEARNTSIDLIELEKTGHMDFLEPQSLAVKRWKMLLYLRYGIHK"
     gene            14575..15138
                     /locus_tag="PK937_04675"
     CDS             14575..15138
                     /locus_tag="PK937_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH31894.1"
                     /translation="MSLLRIIAKPLPDEYPMYSAKYIDLLKNDGLVLYHLMNNFLEIR
                     HNITELSSEILHYRYAPEKWNIKETLVHMMDDERIYAYRALCFARGEKQKLPGFDQDS
                     YTALSGASARSIQSIFVEYEAVRHATVAMFNAFTDEMLLRMGYSDENNSHRSVRAFVY
                     HIAGHELRHWEIIKERYLDRSASCGTV"
     gene            15166..15864
                     /locus_tag="PK937_04680"
     CDS             15166..15864
                     /locus_tag="PK937_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31895.1"
                     /translation="METETFKIDIQNILGKDFAEHELIHSFYGETFALEDQFFPPVVS
                     YALPRILQCESAGVLACSLPDEHAEQMLHYWFGSKDSAYISSGSIRVASHCNSCPDSC
                     TGICPVLMREDPQYFLPPYHVRGYPFSGSIRGHIVLSDPKPLRIHPEGERLIIRTTKR
                     LFNQFKMQFHVDLGADHQAVDALVPICSFCKKIRDDEYQWVRLEKFFNDNYSIQFTHS
                     ICPHCSHEYIKTIK"
     gene            15972..17390
                     /locus_tag="PK937_04685"
     CDS             15972..17390
                     /locus_tag="PK937_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31896.1"
                     /translation="MKSMFLSYFVCFAFVSHTFVVSQIKPSDRVISKVVCQYDTSQSY
                     VLFLPSGYDSSRKYPILYCFKPEKDALYPVVYYADIMERYGVIMVCSWNSQNGPWQVC
                     IDALHAVWKDTHHRFSIDLGRVYATGFSGGARTASELAIRYPHQVTGVIGIGSGFSTL
                     QAIPQAVPFRYFYGLYGNRDYNKSEMIYLDEELSKRQMMHRIVEFEGIHQWAPFTDFS
                     DGFEWMMLNEMRDGKRTPDSAWIAQLFDKRHEQLKTSLHQKNFVDAARMARAIYDDFQ
                     FTMPCSAFVALADSVQKLKAYSDEMALRSDLQQREVHTQNIMDDFLFNLENPLFEPER
                     DMQLQMLYKLTKEWQPLYNTKNEIKSNAAYRCLDYIARLCRARGNGYLNQRVLPKAVA
                     CFDLARTVWPDDASSHYNLACALALSGKTNKALDALETAVKVGFNDVPLLDTDPDLNS
                     VRSDKKFNSIRKKAEHSTTSKK"
     gene            17467..17898
                     /locus_tag="PK937_04690"
     CDS             17467..17898
                     /locus_tag="PK937_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31897.1"
                     /translation="MKWFYVLLFVFVVVFSYCLLQAKQAVMDTSSIEQAVLNYHTYAE
                     WLTYGVPAAFLILLILANFSFTARQHIGSFVYAFLFFIVFTIADYVFLNKLFFEFLNR
                     TGQWRGGNTMMGVFALLLCVMAGGLVLANYLIRYTIKKYKS"
     gene            17895..18986
                     /locus_tag="PK937_04695"
     CDS             17895..18986
                     /locus_tag="PK937_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH31898.1"
                     /translation="MKRLSRRRFLRHCAVQSVALTAGQSAMGMLPEEKTLVEYVRNEA
                     LITVRENYAGNPMRDGIFLNSDLNPNLKPFSVIFKWFLSENPQREEKKNDPFRVTTHN
                     VKDFRAINENGLMWLGHSSFLIRLGNKWLLTDPCLTAAPLQKRYSELPMPISHLESVD
                     YVLLSHNHYDHLDSDTLSTWRSRAMKILLPLRLGSTVRSINKYVTIQEAGWFQKYDLE
                     EDFEIILLPAIHWSKRGLWDNNRSLWGSFLIRTPRTTIYFSGDTAYGEHFGEIARLFG
                     KIDIALMPIGAYKPQHIMKSAHTSPQEAVQGFHDLKAKTFIPMHYGTFDLADEPMGEP
                     VKLLRNMKADGLINGELQIPDAGAWITFD"
     gene            19018..20559
                     /locus_tag="PK937_04700"
     CDS             19018..20559
                     /locus_tag="PK937_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31899.1"
                     /translation="MLPYISRMMIRIYKILLLLCVSSWIAAQSPDELIRRGQERIYTL
                     DFKSADAVFDSLIALDPLHPRGYFFKGSSYFYQILSGHNTRMTGDEFEKWNDKTVEVA
                     EQYAAQAHQETEGDFYRGAAYGNSARYHAVHGEFVKAFYYAKRSKSLHEDVIEADPLY
                     YDAYLSIGMYNYYAAAAPRWMDAIGSLFGLSGDRATGIAQLEKTYRHGKIFNLEAQFF
                     LANVYLEEGDYETSLRIFSQLMDRFRSNPYLINQAGMVSFYLENYSVAEQIFQQSILQ
                     TSQTTRSAEMLASYFLGRMARLRGDYTKAITYFNKSVTIADQCVLFKMVDGWVAGSAL
                     YYLAETYELSGDRTKAEIWYEKARDDARSGKGIVQGAKNRLKYPIGEFEMEFIKIRQK
                     ALIQPEPSQLITLNTMLASASNKPEMAKYVSQIHYYIGRTQFALENYPEAERHFTASL
                     DTQTDDGEEKWRTAFNRYYRGAARVKLGKKSEAKSDLKLLTDDASYVDGNRIRFRAQQ
                     LLKGL"
     gene            20561..22429
                     /gene="dnaG"
                     /locus_tag="PK937_04705"
     CDS             20561..22429
                     /gene="dnaG"
                     /locus_tag="PK937_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNH31900.1"
                     /translation="MAKIPQHTIDEVKNAWDVVELVSGYVGLKKKGHNFWGLCPFHNE
                     KTPSFSVNSDKQIFKCFGCGAGGDVISFIMQYEKTSYPEAIRFLAEKKNIPIPQDHSP
                     EEINQRSEIEELYFINKLTAAFFAEQLTLPQGDEARQYLRKRGFDDAVIAAYGIGYAP
                     DRWDGLFEYAKKESVDPRLLERAGLIIPKTGGYYDRFRHRLMFPISNVSGRVIAFGGR
                     QMRDEPNSPKYLNSPESAIYHKSKVLYGLAQAKDGIRQMDEVIIVEGYADCISLFQFG
                     IHNVAASSGTAFTAEQAQLIRRYTENVCLVYDGDQAGIRAAERGGAIMLEAGLNVKIV
                     VLPGEHDPDSYVREAGESAMQQLVQKGQDYINFRVVEWLKSDKLRTANDKANAVSEMA
                     QIIARMDDSLKRNAFIEEVARKLDTALPLVAKAVQKNRSAVARQKDKEPIVISHAPAS
                     SLTTASSSVPEKVLRAERGILRELLLNPSQCEYVFGYVEPRDFKNPAIQTLIGKIHSL
                     YMEETPYDYNVVVQDCEEKIKTAAERLIFGAGPRQAIVEDKYELSECIREMQLYDLQL
                     EEQTVREAIRVRELEKEDPAELMASLRHIADRIKAVRQSKLISLYETGEHTDEVPF"
     gene            22429..23412
                     /locus_tag="PK937_04710"
     CDS             22429..23412
                     /locus_tag="PK937_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HNH31901.1"
                     /translation="MDTFKFDVVIIGAGPTGLSCAVECARAGLSHVVLEKGCIVNSIY
                     HFPSNMIFFTTSDLLEIGGVPFTTPNPKPTREEGLQYYKRVVQYYSLNVRTGEKVISI
                     QNLSNEFLVDTESNDQQKRYTARHIVIATGYYDQPNKLFVPGEESSKVSHYYTDPHPF
                     YGKNVLIVGGKNSACIAALELYRYGAKVTVIHRGEEIKKSVKYWILPDFMNRVQEGAL
                     TLHLQSVVTHIADNEVTIQNVITRQETIIANDFVFALTGYRPDEHLLRMCGIQLDERL
                     VPLHNPETLETNVRGIYVAGSVSAGLDTNKLFIENGRFHGKTIVEHIRSKE"
     gene            23948..27199
                     /locus_tag="PK937_04715"
     CDS             23948..27199
                     /locus_tag="PK937_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH31902.1"
                     /translation="MNSYTAFLLILLFPAKINSQNLREHDSLRIVRLFQSAENSNKSG
                     NIDNALFYNRYAASLALKYKLWNQYSTAVAQTTKNLIQKNKAKDAISECDSAIQHIIR
                     SDNKSFGNISRLLNNKSIALRRLSDYNQALESVIKAVSLLQENNVEPNKEILADCFGS
                     MTNIYYDMGKYRDAMIANENQRRILSSIFGRNNTRIAGSYSNQGLNFHAIALYDSAIT
                     YFDSSISIHEACGNSESLDYASALSNKGISFFEKGDFKKALLLYNQALELRKRLVGDK
                     FLYIAGNYANIGVTYHELGDYDASIIYMEKALSILNQLNITKHPFIGGIFANIGISYR
                     SKKDYETAVKYVFNALRMRQSLFEMAHPHIGDSYNDLGNIYFDLGDYKSALIYYDSAS
                     YIKSQTTSASSPQMAKLYSSKAALYSITGILDSAKHYYKLAAESWKLIYGEKHYMLSL
                     SIMHLADLEELNNLDSTIHMYQTAICTNTLDFDDTNVFNNPNVESQILSYDVMVQLLS
                     NKARYLLKIYSEKKDTMYVVGAYNALEIAHLLINQKQRTIITDNAKFSNVSIGQDVID
                     DLLETLYMLDEIRGTKNAAEMYKYVVKAKSSIFIESLKDLEITKGVGDGKLLMRENDL
                     REQVSQREIELAKKLESNKFEMNDHVLGIKNDLFDLKEEHASVISELKKTFPSYYNLK
                     YPSSEIDISEIQRGLNDNTALIEYFMGKKKWYVFVITNDDFEVFRFSKDTVFDPTIDQ
                     YLKSVRTINKPEFVSSSYSLYKKLIAPLDKLIDSKKHWIIIPDGKLYSVPFESLISAV
                     QNGEKDFTKLKYLIHRHDIRYHYSAALFLRASENKIVSQSPTFLGFAPIFSDSLKNGH
                     ILTTNTTVLDTLSGIRYRFVTDDSKELMPLPHSETEVANIVSLFEKKAWPARAYFHRQ
                     ATESNFKNFSEAGKFLHIATHGLINLKKPKLSALLFSQPQNTNQGENSINRNGEPVDD
                     GVLYSGEIYNLNLKADLVTLSSCESGIGEYIRGEGMMTLTRGFLYAGSNNILFSLWKV
                     PDESTSRLMTEFYRQCLTNKNYSESLREAKLLMIKKQATSFPLNWSGFVLIGR"
     gene            complement(27212..27961)
                     /locus_tag="PK937_04720"
     CDS             complement(27212..27961)
                     /locus_tag="PK937_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNH31903.1"
                     /translation="MESCSYKNQRSLLIDRYLHNELSEKDRLSFEEHYFICSECFEEL
                     LETEKLKTTIKLGGKELFAVASGLNKRDRIFKYILVAASFALIFFTLYQFFPEKKSGN
                     FSNDSRIALIDTVKKVKEDDSGDKEIHEKTLNPKKNIGFLVENYTPNADFDNIIRQNF
                     RDKNSLQIISPLVDAIISDSVIFDWKPKETKNLELVILNNREEQMQRISINAPFIWNT
                     TTVMPGLYYWKLETSTDLLYIGRFIIKKPLM"
     gene            complement(27962..28504)
                     /locus_tag="PK937_04725"
     CDS             complement(27962..28504)
                     /locus_tag="PK937_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH31904.1"
                     /translation="MEVITTITDCTMTEQNVQELASKLYDAFKEQIRRKVKYRIKRQE
                     EIDDVVAEVYLAIVDSLHKGNFDTSRNAGSYIHRIVENKINTYFRQRKNRLDKFVDID
                     VNELQDNNSNYLEIKSNIEAFKNELSKLKNNHREVLKLRYFEGLSYGEIAKAIGSTEK
                     TVGNLIRYATDKLKKNLKGK"
     gene            28797..29147
                     /locus_tag="PK937_04730"
     CDS             28797..29147
                     /locus_tag="PK937_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH31905.1"
                     /translation="MIHRRLNEQPGCISELAKQIGISRSTLYRLKNGDPKIEARDFTI
                     DRIIRALNISYEIKNGKVIEVENRRLIAVSHDPDEMRLFKEWVMKIDHIVANNLNSND
                     YELIRLLVCRCLKP"
     gene            29430..30503
                     /locus_tag="PK937_04735"
     CDS             29430..30503
                     /locus_tag="PK937_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH31906.1"
                     /translation="MLVENRGIRDIAGFFSTKEGKTFIKGVKTNIFTVVERSKIQQLL
                     KEQNFSMTDAVDANQAAQVGKLLGVDAIITGSVDYSFTDTPEEFKSKKKNGSVKINYK
                     LTRSVAVSAKMKIISVATGEILGNSDKEYKNSDAKMDEERSGIKSVEALVDECLKPMA
                     GELVTYFSPQFILNKYKLQKIKVKEFKKQAEEAGDFFEKGDLNRAYPVYKAVADADPY
                     NASALYNLGLIYDMVGDFEQSYACLKNAYELEPKEEDYQKAFQRTEKNLEFSKVLTSM
                     GIVPEKYDFKIDETALAAKVMIKGDKSDRIEVFSEPKAGSSVVAKIPGDSEFVVLEKN
                     GGWVKLKLLGGKEGWVQKDKVKE"
     gene            30707..32047
                     /locus_tag="PK937_04740"
     CDS             30707..32047
                     /locus_tag="PK937_04740"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNH31907.1"
                     /translation="MKSLSVTLLFIFLLSCTELQKQSLDIIPVKSANKWGYISIDGKF
                     AINPQFSLATYFVDGIAMVRSIENKVGYIGKDGKYIINPVYAKGTVFSEGLACVALDS
                     SGIMYIDKSGQIKINLTNAEFGGIFSEGLAPVALDEKYGFIDQTGTMKINPQFDDARF
                     FKEGMASVAIINEKEGLLWGFVNAEGKTVINPQFKEVGFFQDGLACISNGEKIGYVDK
                     AGIYTINPQFDIPNSGNASFEKITAFYNGTACFRQGREWGFIDKQGKIIINPQFSDVS
                     MAIDGVAPVASSNGSWGYVDKEGKYVINPQFSDASPFWNGNAFVKSGGKYGIIGTDGK
                     FIVNPQFDMILWPSWTWVKTSNRIDFVWVKSPIELRWREINERIMSLYGRLFYYGNAA
                     VSDRKKVDDFLNEADKAVKEKNYFQVKQKYREAIEFMNSIIDSKRTDKRSGGID"
     gene            32047..33942
                     /locus_tag="PK937_04745"
     CDS             32047..33942
                     /locus_tag="PK937_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31908.1"
                     /translation="MRKFKFNIIWLWCFTLLSVFNHYRAHSQCNTWSPVFALIDDKSK
                     SQNIDFRVAASKSGIYVILAPGSIIWVFNDAGRKISTRSGKIQSIAVSDSGHIVIAEN
                     FSIKISRDLGKTWNDISNEPTPFERNQIYPHEIYYVTRNNGKKVEGEIIVKSVKNYGF
                     EASVTLWSTRDGNNWSSETNPGFPSFRSAKHWISTRLGKKLYLSDNGNDVFDCEDSKS
                     QLTSLFTSEEYIKSLEFIGNKSLLIATKNRLYFKNDFSKKSSEWIDLSESFPKTSEIS
                     DLATTDSLIIMTSAGVVYVSTKEKLISGSNSHKWSELKETINGGMDWSGGQIETQKSF
                     ENITAFYKSGNIIAISEWTSIDIAVLISTDNGQRWKTSTIKGKKRKNQEKFYVIFEKV
                     IDIYISGKQIFVLTDEEGHSEGDYVPSGRLYKSVDFGASWETVQEKIRYPRNLYYVDD
                     QLFIVGGKDIYVSNDEGITLKSYSLGLSELSWVRGSSVFDSSIWIATNKSIMKSAFRN
                     IQFNVISSKFTGEVNIADFYPGEKMFSAITSNGLVFHSTDKGKTWKKLSSICELRPAS
                     TYRIIECDSTLFVLGVSIVRYDNENKSWVTISSSLPFKIWSGWKAFDNEPYVFGEGGL
                     WRLKLNN"
     gene            34213..34761
                     /locus_tag="PK937_04750"
     CDS             34213..34761
                     /locus_tag="PK937_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNH31909.1"
                     /translation="MVFTRSVVGNGITAVISGDSLRITKNRFIWPLFSDSHRYGAKVT
                     VIHRGEEIKKSVKYWILPDFMNRVQEGALTLHLQSVVTHIADNEVTIQNVITRQETII
                     ANDFVFALTGYRPDEHLLRMCGIQLDERLVPLHNPETLETNVRGIYVAGSVSAGLDTN
                     KLFIENGRFHGKTIVEHIRSKE"
     gene            complement(34773..35303)
                     /locus_tag="PK937_04755"
     CDS             complement(34773..35303)
                     /locus_tag="PK937_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH31910.1"
                     /translation="MALMLGAIHFSAEKHRHQRRKDPAASPYINHPIQVAELLWRVAE
                     VRDPDIIVAAILHDTIEDTQTTPDEILGLFGKTVLTMVLEVTDDPSLMKSERKQKQIA
                     TAAALSPAAKVIRLADKICNVRDVTHSPPRGWDTLRRKEYYDWTEKVVTGLRGVNPAL
                     ELFYDQVLAEGHALIP"
     gene            complement(35327..35821)
                     /locus_tag="PK937_04760"
     CDS             complement(35327..35821)
                     /locus_tag="PK937_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31911.1"
                     /translation="MRTFLDKNRIAGFIIYPLGDLIAQIIWGEVNGVRIVTMALAGGL
                     IYAFEIPLYFGWINRTFNHWFPRTLAAIAYFNPLWIARHIFLIRASLNPPTILTWPSL
                     SVLLHTSWISGLSSFTGAIVISFAGNYIIQNMIPLERRFIASSIFSGLMAVYYAISEA
                     FLKG"
     gene            complement(35844..36476)
                     /gene="udk"
                     /locus_tag="PK937_04765"
     CDS             complement(35844..36476)
                     /gene="udk"
                     /locus_tag="PK937_04765"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNH31912.1"
                     /translation="MSKKHILIGVAGGTASGKTSVSKKIFETLGGHNQVVIIAQDSYY
                     KDLSQIPHQERAGHNFDHPDAFDNHLLVDHLKQALRGETIRIPNYDHKTHARTSEVTA
                     LEPHKVILLEGILILENPELRDLMDIKVYIDTDADVRLIRRLQRDIRERARTIDSVIE
                     QYEKFVRPMHLQFVEPSKRYADIIIPHGVENVVGVDILMTKIRTLLNEIQ"
     gene            complement(36486..>37496)
                     /locus_tag="PK937_04770"
     CDS             complement(36486..>37496)
                     /locus_tag="PK937_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH31913.1"
                     /translation="ISFTTSFLNQAGALVHIYKDGSVLVNHGGTEMGQGLHTKIAQIA
                     AKELGVPLTRIKVNATNTSKVPNTSATAASAGTDLNGMAVKNAIDQLKLRLMRVAADY
                     FNERALEVRSSPDDIVFEDDTVYDMRYPKRLIKFDELMPITVMKQVSLSATGYYRTPG
                     IFFDREKGEGRPFHYFAYGMCVTEVLVDTLTGEYSFLRTDILHDVGESINPAIDIGQI
                     EGGYIQGLGWCTTEEIKHDAAGRLLTHSPDTYKIPGVQDIPKDFRVALLQNAPNANTI
                     HSSKAVAEPPFMLGLSGWLAIKDAVSAVSHHRYEPEFTLPATNEAVLTAIEKLKPKIK
                     PQ"
BASE COUNT        11215 a         7010 c         8351 g        10920 t
ORIGIN      
        1 taaattttcg agtcgctcct cccatgtggg gaagaggatt cattcaagaa ataccggctg
       61 aaacgattgt cgccaacgct gatcctaatg atgctaatga tgatggtatt tcaggacgag
      121 cgaattacat tgttatgagt tttgttgatt ccccgacaca ccccgaactt gggcgtcacg
      181 gcttaaaagc gcaaatgtcg cgaattttag attttgccga caatgctttc ttgcatgatc
      241 tcggaatgac gtcacctatg cgaccgtatg aattagttaa tcctaatgcg cgcgatgcaa
      301 aagataaaaa atcaggtgta gacattagtc atgaagatgt agaaaaagta gattttttta
      361 tacgtacaag cgattttccc cccattttac aagaaacgga acagattcaa attggtaaaa
      421 atttgtttaa tgagatcggt tgcaacaaat gccatattcc ttctatgaca accggtacat
      481 ctgaaatttc tgctttaagt aataaaacgg tgttttttta ttccgacttt ctgcttcatg
      541 atatgggcca aactctctcg gatggtgtat tagatggaga cgctaaacca caagaattca
      601 aaacacctac ccttaggggg ttgcgtttct ttaaagactt ccttcacgat ggacgtgcca
      661 aaaatgtaga tgcagctata cgaatgcatg ggggtgaggc aacgaccgtt cgcaataatt
      721 ttgtaggatt gtcacaagaa gagcgtgatg caatcatagc atttttaaat tcaatttaac
      781 taatacagga ccttgcttta aatatgaatc gttcaaaatc attgctaata attttaattg
      841 cggcattagt tttaattcct tcatgcgata atgtagaaag cggaactttc agtggtattt
      901 attttggtag accaccgcga aaattacgag atgggcaagg atatttcgaa atgtggctag
      961 gttttcctgc atcaagcggt ggtaaagaat gggtaagtat tttaaagttt gatcgagtgg
     1021 agagtactaa taagccattt ttaaacgata aagcagcaat actaaaaaga cctaatcgaa
     1081 atatcaacga tgcagtttca gcagccattt ctattgaact tgaaaatgat atagatcctt
     1141 ctgtgcccaa cagaatattt atgagcggag aattcaccga tggtattgca tctttggaac
     1201 tcagcggtga agacgcttta aatctggatc tgagtgatcc agccgtcatt agcgggaaat
     1261 tcgtactagg aactccttcc gatgcaataa ataataatga cgacagcggg gtctggtttg
     1321 ttgatgcacc atttggtcaa caacaaaatc cgggtttatc gcttcctgta ctacccatcg
     1381 gatttaaata tgaggcttgg atcgctacgc aatccgaaac agtatgtatt tcaaccggtc
     1441 aattcaaacg tgcggacagt ttggactata acggtaaggg acctaatgga ggcggtgaat
     1501 tgactccaaa tgttccggga gaggatctag tatttaatca attcaatgaa gacgtacagg
     1561 gtcgaactgt ggatgcttcg ttacaagaag catatctaga tacatttgga tttccaattc
     1621 atattaaaaa aaccattcag aaaaatcctt tgactaacgc taaagattat tgggatgtag
     1681 tagtgtctgt tgagccggat ccagataatg accctgaaga accgtttgcg atttttcctc
     1741 ttgttaaatt tggtccaatt tttgaatccg taaatacagt aattcctttg actaatttta
     1801 ccggaggtgt gtctcaatac attgttattg atttatcaaa aatgtgaaat gcccatattt
     1861 gataaattaa atgaaagcat atttgtcaaa ttcatatagt taggtgggga agttagacgc
     1921 agtcaatatt tacattgata tgaaagttaa aaccactaga aagcctggct caattgattt
     1981 tgatcactag ccggtcgaat tgctgattaa ggtacagatt tcaataagtt taatttttct
     2041 atattgtacg atagacgtgt tttgtgttat caaaagctat ttgaaatctt agatggcgct
     2101 agggttatga tatctatagc agccgtagag gttggtatgc tcaagcaata caaaagcttt
     2161 taatcgggtg acctcgtaag tttttctgca tatcaggaaa tattctgcac gcgttgattt
     2221 tcaggctcgt agcggcatag tatttatgat gacatggata aagaaaatgg aaatatcctt
     2281 tagacctttg tttctaaatt cgttttttta tcaatgggaa aaattttatt aatctgattc
     2341 ataatcattg atgctacgga attacaactc aattgacaat aagccggtct agcttatcaa
     2401 gctaattctc ttacaaccac cacccatcca aactcatacg aaaactgcgt ttacggaaag
     2461 ccaaaagttt gttggaatca caagccggta atgtgacgca ggtcgcctat gatgtcggtt
     2521 ataccagttt gtcacacttt accaaagctt ttcgcgatga agttgatata aattcctccg
     2581 atacgatctg atctaaaagc tttaaaaaca atctccgcaa cataagttgc aacaaaaccg
     2641 ctaataattg acccactacc gttagcgcgc atctcgtctt atctgtaaat tttcactatc
     2701 aaaatttgat tgagttaatc tggattaact cggacttaga gttgatctat tattattaat
     2761 agttaaacag catcgtgaat ctttgattcg gtaggattgt gtaataatac tcatgtgctc
     2821 atgtggaagg aattcaaaat gttttggaat tggtttttag cgatcggact atttgtgtcc
     2881 ctcagtaata tccttttctc gcaagcgcag gatagtattc gtattcgcac cgtacccgtc
     2941 gccggtaaag tgtggatgat ggattgtgaa aacggatttg gcggtggtaa tgtagctgcg
     3001 atggccggtg atgaaggtgt cttgttggtg gatgcgatgt acgcctctat gactgaaaaa
     3061 ataaagaaag cggcctttac actttccaat ttacccatcc ggtgggtggt caattcgcat
     3121 tttcatcgtg accacatcga aggaaacaaa aatctgcgta catctgcggt tattataggc
     3181 caagaaaata tagctaagcg tttgcgtagt aagcacacac aaactacacc gactctggat
     3241 atgatgccga gcgtattttt ttcagatagt ctcactctca ccgtcaatgg ggaagatatt
     3301 cgcattatcc acatgcccgg aagtcacacg tcggccgatg tcgtggtgta ttttacgcaa
     3361 tccaatgtac ttcatctcgg tgatttattt tttcatggta tgtttccggc cgtgtacact
     3421 gacggcggtg gaaatattca tcaattgatt gcatcgctgg acaaaataga aaagatgttt
     3481 cctgtggatg tcaaaatcat cccgggacat gggccattgg ctacgatgcc agcactgcat
     3541 gaatatatca ttatgctcaa agagaccgta acagctgcgc aagaaggcct tcggaataaa
     3601 caaacttcgg atcaactttt gaagacctcc gtatttcaaa aatataatta cctcggtgac
     3661 ggaggcgctc agactacgga gcagtatata aatatgctat tcaaactact ttcagaatca
     3721 aaataattct catcgcgagc cgtatatcca catcaaataa gggattccaa tgaattatat
     3781 gacggttgtt atgtcgcgca cgttgtgtca agcgcgagtg tttttatgga tgtttattgt
     3841 gctttgtatt caaagtgtag ggatgtattc ttcctacgcg cagcagtctt ttttggtaac
     3901 caatggtacg gatggtccga atccgggccc tgaaggatcg ttacgacgcg ccgtatatga
     3961 agcgaatacc tggccgggac aagatattat tatcattaat tgtagcgata tcctcttgac
     4021 gtccggttcg ctcatcgtca cggagggtgt aagagtaagt tcaggcgaac tgccgtcaga
     4081 gcatttgttt aataattggg gcttgcatgc ggatgatcct ttgaatgaca cactatggac
     4141 gattcaagcc ggacagatgg ttgagataat aggactcaat atgttcaatg ctaagattgg
     4201 ggtttatatc gcaagtaacg gatgctttat cacgtataat aaaatatcaa actgccgtac
     4261 cggtatttac atcaaggggc aaaataatgg tattcttaac aatgccttcg gttttgcaaa
     4321 gccgtatccg tattccaatt atgcggcgat cgagccgaac ggtacggcca tagcggatca
     4381 ttcgtcgggg tttaatacgt ttttgggaaa ttattttgcc gcgagcagtg aaacagaatt
     4441 gtatatcggt gctacggcgc gtgaagacgg cgtgtacggc aactattttg gattcaacaa
     4501 tccgagcatt caatccgata cggtattcag ctataaggcg cttactattt atgggcaagc
     4561 gacaaatgtt ggtgggaaac gcgatggttc gtatatcaac cccaatgttt ttgcaagcta
     4621 catgagttac ggtgaagtgt attcggacag taatgttatc atgggtaata tgttcggatt
     4681 gcagcatgac ggccaaactc cggcgccatt tttggccgcc ggcttgacga tttatggcga
     4741 cgataacctg attggccacc acacacgacc gggacgtaat gtatttggca gcggtgaaaa
     4801 cgcgtcggca ttacttctta tacaagcgca tcgcaatacc atcatcggta attattttgg
     4861 cacggatagc acgggtctca gcgcaggtct gcttaatcgg ggaattcagt tggtgtcggg
     4921 gagttcagaa aatattattg gcggagcgac ggtcggagaa aggaatattt tttccacaac
     4981 gcaagtggat atttatgttc aggacggcac cgccaatcat attcttggta attattttgg
     5041 tttgaatgcg gacggaagcg ccacgttagt acgtggcgga attggtgttg acaatgcatc
     5101 acccgggttg atcgtaggcg acggcagcga tgacggacgt aattattttg tcaataaaag
     5161 tacagcaatc aacacgtata catcttcccg cgctaccgaa gtgcagaaca acgttttcgg
     5221 gcttatgccg gatggtgtga cggtagagcc aaacaatacg gcgctggttt tgagtggaaa
     5281 tgccagtgtg atagacgata atattttcgc aggaaccaat gccgggcgtg caatccaggt
     5341 gtttgccgat tcatgttttt tatcaggaaa cataataggg gttgatgtat tgggtaacgg
     5401 tcccaaagga tttaatatag cgttagaaat caacggagaa aataatcgca ttggatcgcg
     5461 cacatcaggc gaaggaaaca gtatctcctg gtccacgcaa ttgatttcta tcgggagcgg
     5521 ggcgaatcag aatcatatcg aaggcaatgc tatcggtctt ggcatcgaaa atcaaatacg
     5581 cggcggtggg cacggcatat ctgttaatca ggctcgcgga aacaccatcg cttataataa
     5641 aatcggttcg atagttaatg cgatcaattt atcaaatacg tataacacac atattcacca
     5701 taatgctatc ggcgtagccg attcattggg tagtattcag cgcgggaata gcggcgttgg
     5761 tattagcatc acatcgaata catccggtac tttgatagaa cataatacca tcgcctataa
     5821 taatatttca ggtatcagta ttgtcgatgc agaatcggac agtaatcgtt ggtattggaa
     5881 tcgtatttat gaaaataaaa gcggtattac acggccttca aattcaaaga acagcgtaac
     5941 gcctcctcac atcatgggcg gcatcgcgaa tgtaggcgtg tggggaaaag cacaaccggg
     6001 ttcccgtgtt gagctatttt tagatgagcc cggaaaaata caagcttcag atattatcgg
     6061 cggtacgtac gcggatgcac aaggcacgtg ggctatttct gttccgggat tatcggaagg
     6121 tcaaacgctt gcggccacac aagacagcgc gttcaattcg tccctatggt caacgcgcta
     6181 tatcccttca tcggcgggta atatactgga gcgtgatcat ggctacagag ctgtaatttc
     6241 tctcggaagc atcaaagcat ttgatagtat ttccgttcca ttgcggtttt atgcggatga
     6301 cggttccgtt atttcgcaaa ttgcgatgca ttccggtgat gagtttagtt gggacggaaa
     6361 aaatgcagat acgctggatc atagttaccc cgatacgttg atcggatggg tcaaatttaa
     6421 acctgaatcc gaaggtgttt tttcggacac actacgtgtc gtgtacgata gccgagtatt
     6481 tgcgttgacc ttggagggcg tcgcgacaga accttcggta cttacatcgg aagaacttca
     6541 atatagtttt cccaatacac gcgtaaccta tgtctcgaat cgtcaggtag aactgtatgc
     6601 tcccaaagcg cctctaaatg taacggctat tcgattagcg cgtgccggaa catccggtta
     6661 tagttttgat ggaagcagtt taccgtattc gatcgatagc gatacgttgc gcgtgggtgt
     6721 acattttaca ccgtcggcga taggtgtatc tctggatacg ctcattttta gtcacgacgg
     6781 ataccagcgc gaactgcgaa ttcctttgca gggattaggt ttagaaaatc aggcaccggt
     6841 ggtgcacacc ggtcttttgc gaagtacggt tctaaaagat cggattgagt tttacgctgt
     6901 atcggatgaa ccactatcgt cggtttcgct aaaaatcaat aacacgacag tcccgctaca
     6961 gatcgtaccg gcgcatacgc gtatgtatcg agctgaatac gaaatcacag ccgacgggga
     7021 attggctttt gaagtgacgg ccaatgatac gatcctcaat caagccattg ctctcaaaac
     7081 atatgtcata gccgatctgg gttccggcag tacaagtgta cttcgttccg gtcaagcgga
     7141 aatgcgtatc ctgaaatcat cagcgcgcgc cgaaggttat ttgatccttt cagagatttc
     7201 atcggacaac gatttgacca ataacggatc aaacattttg caaacctgcg aaattttatc
     7261 aaccgcttcg cagtcgtggt cttcggagtt aagttttaat atcgccccgg cacatcggag
     7321 tgattggaat cttacaattc ccggattttc tacaaaacat ttgaaaatta cacgattaga
     7381 tgctacgggt ggttgggaac gtcgtatgat cgcgcacgcc gatgaaaacc gagtatatac
     7441 cagcatcaat gaaagcgggc gatatgcgct cgtatatgat ccatcctttt cggagatacc
     7501 taagtcgtta gatttggcac agaattatcc gaacccattt aatccgagca caagcattcg
     7561 atttggcctt gcaaatgatg gctatgtgcg catcgccgta tataatgtac ttggtcaaaa
     7621 gattcgcaat ttgatttccg aacagcgaac agccggttat cataacgtga tgtgggacgg
     7681 gaaaaatcag aaaggatcgg cggtgccgag cggtatctat atctaccgat tagagacggt
     7741 tgacggcata ctttccaaaa aaatggtatt actcaagtaa acatcaaacc tataattaca
     7801 tgaaacatat aaaacatatt tctaagccga ttttattttt gtcattaatc ctgacctgtg
     7861 ccacgggtgc ttgtgacgac tacgaagcaa gtttactgga tgctgcctgg aaaaaatttg
     7921 aaaatcaaga ttatgacgga gcttatgctg aattttcagc acttaatggg cccaatgcgc
     7981 gtattggatt aggatggacg ctgcttcgta tggacagtat tcccgaagcg gatcgtgctt
     8041 tccgtattgc cgctgccgat tccgtattag acggatacgc agggattgcc ataaccagtt
     8101 gggaaacggg ccgatatgct tcggctatct atgcagccaa ttttgtcgaa cgctgggatc
     8161 ccgaatacta ttttgtccat gatccatccg ttgaactcaa tgatcttata ctccacaagg
     8221 cgttttgcca atactatttg aatgatctta ctgcgtgcaa tgccagtata aaaaaactgg
     8281 atccggcttg ggttacatct accaatccgg ctacgatact taataagctg gaagtgttgt
     8341 atgatcagat ggattgaaat aaaacaaaaa taaccctcct cccatgagaa ccgaagcccc
     8401 tctttctgct gcaaagcaga aagagggtaa cttcgctaaa tccaaaggca taaaaatgta
     8461 taaagtactg ttattgattc tcgtcatggt tcctttgaca ctattcggac aaatacctga
     8521 cggtaaaaaa attctggata aggctaagaa aaaaaacaaa gaagaaagta aaaaagaagt
     8581 taaggacaaa tcaaagaaat cagacgatcc gtcaaaatcc actgcaactg aaaaaaaagc
     8641 aggagagatt cctcgaatac aacaaggttc gaccgctgca aaagagaact ccgaatcaaa
     8701 aaaaagcact catgatgatt atcaaaaatt gacggtatcg gcggatagcg atcctttcga
     8761 agtgaagacg ttaaaaacgt atatcgttcg catgctggaa tgtttgacgg aagtcgaaac
     8821 ggataacagc aaacaggatc agaatattgc cgatcatgta ttttcgaagt tcgagataca
     8881 tcattctcgt gcggaggaat cattaacaac agtacgcaca ggaaaattgc ttgccaatgc
     8941 cgatgtctat gaatcggcta tgcgcgaaat caaacaagat tttgaagaag cgaaaaaaac
     9001 attcaacgaa aaatcgcgat tcatttcagt tcagtccaaa attcggcgta tggtatccga
     9061 tgtacaagat caggtcgcgt cattgaaaca gcgctccaat tatgatagct acatgacttc
     9121 gcaagcgaaa ggcgatttag atgaagcgcg cacggcactg caaaatgcca ttaaagaatg
     9181 gccgcaactt agtacatcaa gttatgctgc ggatttattg caatatgaaa aagaattaca
     9241 ggctattgaa tcgtccggga atgcgagtga ggggtttaaa aagctcatag ataaagcctg
     9301 gttcgagggc aaatttttgt tgcaaaacgg attgcaatat gggtcattca cgaaagatgc
     9361 agcacaaagt ttttatgaaa actgtgccaa aataaattac cccaaagcac tcagtgcatt
     9421 aaatagcgcg aaaccaaaca acgatgtcaa ttcgtcacaa atcgaatatt taacaaaaga
     9481 atttccgact gaatttaatg cgtatctcaa aaaagggtat atcgctgata tcaattcgca
     9541 tattgaaaaa gcctacgcat ataaagccaa tggtaaaaca cagttgagtc tggccttaga
     9601 agaagccgaa acggccgtaa ttatggccga tgccgtgctt ttagtaaaac ccgatgatgt
     9661 ttcgatccaa cgtttgcgta aggatgggtt atcaactgtc gaaaaaatga aaacagaaat
     9721 tgatgccgct gaagcgacag cttttacaag cgtctttcat aaagaaaaca aatctaaaat
     9781 tgttttttct aaaaatagaa tttctctcaa acaggaaaac gcgtcaactt catccgattt
     9841 tttcaacggg ggtgaaccga tatacgcgat ggcttatttt accgcaatgg tcaaggattt
     9901 acccaaaatg taccgcacgg ccaatccagt ggtgcgtatc aaatattatg tagatgtgcc
     9961 ggcggatgat gcctatgccg taccaaccgc tacgactgaa tatcaaatta tggcttccaa
    10021 taaaaccttg atggaagcta cgaccttgga agtggaagtg gctcccaatg cggggcaaac
    10081 cgatcgtcca gaagtcggcg ctcaagccgc taagatgtta tctcaactat ccccacgttt
    10141 gcactcaatc cgagtggtat tgagtattga taatgaaaac tttgcaaccg gatcgtttga
    10201 gcttgatggt tcgcaagggt tagaaaaatt tgcagctttg ggtgacgact taattaaatc
    10261 taaaatacag aatgcccgtc cgcctaaagg aggagggctc gaaaaaacga ttgaacaagg
    10321 cattcgttcc gctttgaagt cagaaggatt taatgtgtta cgtgtgattc aaacggatcc
    10381 gcaatggtac tatgaacgac acccgattag cggtgcgata cttaatcgta cgatacaagc
    10441 cgccattatt tataaagacg aacagaataa gtgtatcgtc gaatacatgg agtttactca
    10501 gaaatatatc aacagccaat acgatgcttt ataccgcagc atgcgcggta ctgtgacaaa
    10561 gccgtacgaa gtgttgcctg aacatgtgaa cgagtgagag aatgttaata aagcagaata
    10621 cgtataaaca agtgatctaa aaagaagtga attaataatg atcaagattt tactgtcgaa
    10681 atggcctcgt attcattggc atgtttttct gttgggtttt gctatactct cctgcgatag
    10741 caaagaaaca aacgaggata aagtagagcc tgttaatcca tatacagacc tgcgttggcg
    10801 agtttcttta accaaagtag ctaaggccat tactagcaat ccggtcaatc ccaatacaat
    10861 ctacgttaat acttatagtg attatgtagg gcgcggtgga atttttatta gttatgatcg
    10921 cggcgaaacg tggcctgccg ctatgtctcc tacaattgac acatcaacga atcagggagc
    10981 gggcgatgta cagtttatag ccgtatgtcc taatgatact catatcgttt ttgcgggagg
    11041 cgttcattca tcgggcttgc tccgaacatc aaatggcggg aagaattggg ttaacgtcct
    11101 accgggtgtt gatgccgatg ccgaggcgat ggcttttaat cctcaaaatc cggcacaggt
    11161 ttttttcatg agttatacag atgccggttt ttatgtaagt caggatacgg gacggacgtg
    11221 gcagttgcgt aaatttacgg gtttcggata ttcgtgtact ctacgaatgc atccttcgga
    11281 tccaaatatt ttcgtagccg gagcttctaa aggaaaaata ataaaaacca aagatcaggg
    11341 ttatagttgg tacttagtac agaaacaagt gtccaatcaa cctaatgagt ggcctgaggt
    11401 acctgcaatc atatgggata atgaaaatcc aaataaacta tatgctacta tttccggcgc
    11461 taatggaaaa tccttttccc aaagtaataa tggcggtgaa acatggcata taaaggattt
    11521 gaaagaagag ttatgggctg cgcatatgac gaagagtaaa cttgttatat tcggaggatg
    11581 gcaatccact tatatcacca gtgatcaagg aaaaaatgtg tttaaaatcg gaggtgatga
    11641 aacggtgtcc gggtattggt ccatcacatc atctggaaat aggattttgg cgattagctc
    11701 gtacgatcgc agtctcatga tggttgactt ataatgcgat gttgaagatt attataactt
    11761 aataacgaaa gaattttcta tgaaattgtt aatacgtggt attttaataa tgctgttggt
    11821 ttctggttgt agtaaaaatg aacaacagga aaatgccgat aaattcggcg aggggattac
    11881 tctcaaaaaa gtggtaagga tgccggaagt gttaaataat cccgaaacat acgacgggca
    11941 agaaatcgta gtagaatcaa cgataaaaac ggtatgccaa gctcgtggtt gctggatgga
    12001 agtcgaatcg ggaatattgc caatgcgtat ctcatttaaa aatgatagtt atttcattcc
    12061 caaagatgca gcccggcgta aagtaaaaat gcaaggcgta ttgaaagtca aagaagtttc
    12121 cgaagaagtg gctcgtaatt atctcaaaga agcaggacgt tctgatgaag aaatcggaaa
    12181 gataaacggg gcacaaagac ttcttaccat ggtagccagt ggtttggcta tttataaata
    12241 atttgatttg accgtcaata ttcttatcac cacttcgtga tagatggttg tagctcatcg
    12301 ttacagttca ttattaaaag gagtctaata tgaaatatat cttagtaata gcattacttg
    12361 tttctcttgg cagacaatct ttggctcaaa cagaaacaat tgaacacggt gttggttttt
    12421 atgtcaaagg tggctggttc ttcgatcgta gttccggatc agaagaccat tcccctgaat
    12481 taaaaggtct caaaggcgag ctcggaaatg acatatcgga tcgctcggtg tatattggag
    12541 tgcgatataa aaaaattgag gcagaaatgg gctggggaga aatcataaaa aatagtgata
    12601 ttacgattta tcccgttgaa tcacgagctg cgcgttattg ggccggatct gttgcatact
    12661 atgtgccatt tcaaattggc tcatcgaatc cgatcaaatc caaattagct ttgggtgtaa
    12721 aatactccac gtacaatact agctatgtgg atgagagtat cacgaaacta tccattgata
    12781 aatggaatat taaatccacc tggtttccgg aacgaagcta tttcagtttt ctctttccaa
    12841 cggtttcctt tttttatgaa aatcaacaga aactagctaa tcagtcggag ttcggtcctg
    12901 cgcatagtat tggcattgcg cttaaaccgg ccgccgtgtt tcccttgggt attttaccgg
    12961 taaaaatcgt gattgaaccg ggaatgtcgt ggagtcataa attatcaggt agcgatcatt
    13021 tccaaaaagt ttatgatacc gtgtattctt tagatgctgc gatagtatac aatcttaaat
    13081 aatcttagat aatgtttcag tagtttgtat ggagaaactg tgtatgggag aattgaattt
    13141 cttggctatc gcggttgcgg ggtttgcata ttttatgttg ggcgctttat ggttttcgcc
    13201 atttgtagcc ggtacatatt atgataaagc gctgggattt acacgaacca aagatatgaa
    13261 atggggcgtt atctattata ttggtccgtt tatgggatgc gtaatttcaa cctttgcaac
    13321 agccgtgctg atgcggtggt gttccgttat aagcatggga gaagcgacaa agttgggttt
    13381 aatcgttggt atgggatacg gtatggttat ttccattacc aatgccatta acccaaatac
    13441 gcccaggcca ctcatgtatg gtttggcgac aggcggttat catgtgattg gaaatatcat
    13501 ggtttctgtg ttaatttata ttatgtgatc caatcaggtt gcattggttt atggagaatc
    13561 aaattttaaa aaaattaatt tatgtcctga tattacaatc atgatttcca tccgcttgat
    13621 ctcactgtta gttgcttttt ttattaataa atcttttttt gatgtaaaca aaactttgat
    13681 ggccttttca gtaaataaat caggtgttat gtcagccgat tataatcggt tttcagaaaa
    13741 gtatggcgat catttgtcgc aagaaggcga tctgtatata cccatcgggc aacccaaagc
    13801 catagtgtgt ttttttcatg gaggattttg gcgcgcccct tatgggcgtg atcagtttga
    13861 tagcgtagcg attgattttg ctcaagccgg ctatattatt tggaatatcg aataccgccg
    13921 tgtgggtgaa gtcggcggtg gttggccggg tacatttgaa gatgctattc tatctgtaaa
    13981 ttatcttagc acgttacaaa aaaagtatcg ttggagcgat aatttgaatg ttgtactcgc
    14041 cggtcattca gccggcgggc aattagcgtt gtggttaaca gctcacgccg aaaaaaaatt
    14101 tcttcagcat acgccattga ccatacatcc ggatttatgc atagggctcg cgccggtcgt
    14161 aaattttgaa aaagcgtatc aggaaaatct tggagggcaa gcgatttata aattactcgg
    14221 ttgtactcca aaggaaaatc ctgaacggta tacatgttat gatccgttgt ttgctgtaca
    14281 tctaaatacg ccgcaaatta tcttgcatgg cgagatggat gaagacttac ctattgcgta
    14341 ttccatatct taccttcgtg cagcagaagc ccggaacacc tctattgatc tgattgagct
    14401 ggaaaaaacc gggcatatgg attttttaga acctcagagt ttggccgtaa aacgatggaa
    14461 aatgttactt tacctgcgct atggaataca taaataacta attgatcagt aaggctatac
    14521 acgaacttat gtgatttttc ttctgaataa ccctaactga tctgaaaatt ttatatgagt
    14581 ttgctaagaa ttatagcaaa gcctttgccc gatgagtatc cgatgtattc ggctaaatac
    14641 attgatctat taaaaaatga cggccttgtt ttatatcatc tgatgaataa ttttcttgag
    14701 attcgtcaca acattaccga actctcttca gaaattctgc attatcgtta cgcgccggaa
    14761 aaatggaata tcaaagagac gttggttcac atgatggatg atgaacgaat atacgcatac
    14821 cgagcgcttt gctttgcgag gggcgagaaa caaaaattgc ccggttttga tcaggatagt
    14881 tataccgcat taagtggcgc gagtgcacga agtatccaga gtatttttgt ggaatacgaa
    14941 gccgtgcgtc atgccaccgt ggctatgttt aatgccttta ccgacgaaat gttattgcgc
    15001 atgggatatt ccgatgaaaa taacagtcat cgctctgtgc gtgcttttgt ttatcatatt
    15061 gccggccatg agttacgtca ttgggaaatc atcaaagaac gttatttgga tcgctcggcg
    15121 agttgcggta cagtctaacg aaatgagtta cttgttttaa tcttaatgga aaccgaaacg
    15181 tttaaaattg acattcagaa tattctcggt aaagattttg ccgaacatga actgattcat
    15241 tcgttttatg gtgaaacctt tgcattggaa gatcaattct ttcctccggt tgtttcctat
    15301 gcactaccac gcattttgca gtgtgaaagc gccggtgttt tagcatgttc attgccggat
    15361 gaacatgcag aacagatgtt gcactattgg tttggaagta aagattcagc gtatatttca
    15421 tccggaagca tacgcgtagc atcgcattgt aattcatgcc cggatagctg cacgggtatt
    15481 tgccccgtat tgatgcgtga agatccgcaa tattttttac ctccgtatca tgtgcgaggt
    15541 tatccttttt caggctccat acgaggccat attgtattaa gcgacccgaa acccttgagg
    15601 atacatccgg aaggtgagcg gctgattatt cgaaccacca aacggttgtt taatcaattc
    15661 aagatgcaat ttcatgttga tttaggtgca gatcaccaag cggttgatgc attggttccg
    15721 atttgctctt tttgtaaaaa aataagggat gatgaatatc aatgggttcg cttggaaaaa
    15781 tttttcaacg acaattatag tattcagttt acgcattcta tctgtccgca ttgttctcat
    15841 gagtatataa aaaccataaa ataattttct gaaaaatctc ttaaagtgat gctgattgtt
    15901 ctatataaat ttgcatcttt ttgttgttag gatcgtacat tgatagcaat tacataagtg
    15961 aattgtatgt tatgaaaagt atgtttttat cttactttgt ttgttttgca tttgtgagcc
    16021 atacctttgt tgtatcgcaa ataaaaccct cggatcgcgt tatttcaaaa gtcgtgtgcc
    16081 aatatgatac gtcgcaatcc tatgtacttt ttttaccttc agggtatgat tcgtctcgaa
    16141 aatatccgat tctatattgc tttaaacccg aaaaagatgc gctgtatccc gtagtatatt
    16201 atgccgatat catggagcgg tacggcgtta tcatggtgtg ttcatggaat tcgcaaaacg
    16261 gtccgtggca agtatgtatt gatgcgttgc atgcggtttg gaaagatacg catcatcgct
    16321 tttcgatcga tctgggtcgt gtttatgcga ccggtttttc gggcggtgcg cgtacggcgt
    16381 cggaattagc catacggtat ccccatcagg tcaccggcgt tatcggcata ggctcgggtt
    16441 ttagtacact ccaagcgata ccgcaagccg taccttttcg atacttttat ggtctctatg
    16501 gaaatcgcga ttacaacaaa agtgaaatga tctatctcga cgaagaacta tccaaacgtc
    16561 aaatgatgca ccgcatcgtc gaatttgaag gcatacatca atgggctccc ttcactgatt
    16621 tttcagatgg gttcgaatgg atgatgttaa atgaaatgcg tgacgggaaa cgaacaccgg
    16681 atagcgcatg gattgctcaa ttatttgaca aacggcatga acaattgaaa acatcacttc
    16741 atcaaaaaaa ctttgttgat gcggcacgga tggcgcgtgc catatatgat gattttcaat
    16801 ttacgatgcc ttgctctgca tttgttgcac tggccgatag cgtacaaaaa ttaaaagcct
    16861 attctgacga aatggctttg cgatccgatt tgcagcaacg cgaagtgcac acgcaaaata
    16921 ttatggacga ttttttgttt aatttggaaa atcctttgtt tgaaccggaa cgggacatgc
    16981 agcttcagat gttgtacaaa ctaacgaaag aatggcaacc gctatataac accaaaaatg
    17041 aaataaagag taatgcagcg taccgttgtt tagattatat agcacgtctt tgccgagcgc
    17101 gcggtaacgg atatttaaat caacgtgtct tgccaaaagc cgtcgcttgt tttgatttgg
    17161 cgcgaaccgt atggccggat gacgcctcgt cgcattataa tttagcatgc gctttggctt
    17221 tatccggtaa aacaaacaag gctttagatg ctcttgaaac ggccgtaaaa gttggattta
    17281 atgatgtgcc gttacttgat acggatcccg atttgaatag cgttcgttcc gataaaaaat
    17341 ttaatagtat taggaagaaa gctgagcatt ctaccacatc taaaaagtaa ttttacgata
    17401 accgaatttc ttgccttatt tctgacaaag gcgtaggtta ttgtgaaata gtatgggatg
    17461 tgatgtatga aatggttcta tgttttgttg tttgtttttg tagtcgtgtt ctcatattgc
    17521 ttgctccaag cgaaacaagc cgtaatggat acttcatcta ttgagcaagc cgtgctcaat
    17581 taccatacgt atgctgaatg gctcacctac ggcgtaccgg cggctttttt gattttactt
    17641 atacttgcca atttttcatt cactgcccgt caacacatcg gatcgtttgt gtatgcgttt
    17701 ttgtttttta tcgtttttac gatcgccgat tatgtttttt taaataaact tttttttgaa
    17761 tttttgaatc gtacaggtca atggcgcggt ggaaatacca tgatgggggt ttttgcattg
    17821 ttgttgtgcg taatggccgg cgggcttgtt ttggctaatt atctgatacg gtacactata
    17881 aaaaaatata aatcatgaaa aggctatccc gacgacggtt tctccgacat tgtgctgtac
    17941 agtcggtggc gctgactgca ggccagagcg cgatgggtat gctgccggag gaaaaaactt
    18001 tggtcgaata cgtgcgcaac gaagcgctta tcaccgttcg tgaaaattat gccggaaatc
    18061 cgatgcggga tggcattttt ctgaattccg atttgaatcc caatctcaaa ccgttcagtg
    18121 tgatttttaa atggttttta tcggaaaatc cgcagcgcga agaaaagaaa aatgatccgt
    18181 ttcgtgtaac aacacataac gtgaaagatt ttcgcgcaat taatgagaac ggtttgatgt
    18241 ggcttggtca ttcttctttt ctgatccgcc tgggtaataa atggttactt accgatccgt
    18301 gccttacggc ggcgccgttg cagaaacgtt attccgagct gccgatgccg atttcacatt
    18361 tggagtccgt ggattatgtt ttattgtcgc acaatcacta tgatcatctt gactcggata
    18421 cgctgagcac ttggcgctca cgtgcgatga aaatactgct gcccttgcgg ctcggttcga
    18481 cggttcgatc cattaataaa tatgtgacca ttcaggaagc cggttggttc caaaaatatg
    18541 atctggaaga agattttgaa attattttac tcccggctat tcattggtct aaacgcggat
    18601 tgtgggataa taatcgctca ctgtggggaa gttttttgat tcgtacgccg cgaaccacga
    18661 tttatttttc gggtgatacg gcctatggag agcatttcgg cgaaatcgcc cggttatttg
    18721 gtaaaattga catagctttg atgccgatcg gcgcgtataa accgcagcat atcatgaagt
    18781 ctgcccatac cagtccgcaa gaagcggtac aaggatttca tgatctgaaa gcaaaaactt
    18841 ttattccgat gcattacggt acatttgatc ttgctgacga accgatgggc gagccggtca
    18901 aattacttcg aaacatgaaa gccgacggat taataaacgg tgaactgcaa ataccggatg
    18961 ccggtgcttg gataaccttt gattgagcat gccgtgaata gcctttgtgt ttagtgtgtg
    19021 cttccttata tttcccgcat gatgatccgc atttataaaa tcctgctgct cttatgtgtc
    19081 agctcatgga tcgccgcaca atcgccggac gaattgattc gtcgcggtca ggaacgcatt
    19141 tatacgctgg atttcaaatc ggccgacgcc gtgtttgatt cgctgatcgc actcgatcct
    19201 ttgcacccgc gtggttattt ttttaaaggg tcttcgtatt tttatcagat tttatccggt
    19261 cataacacgc gtatgacagg ggatgaattc gaaaaatgga atgataaaac ggtcgaagtg
    19321 gccgaacaat atgccgcaca agcccatcag gaaaccgaag gtgattttta tcgcggcgcc
    19381 gcgtatggga attcggcacg atatcacgcc gttcatggcg aatttgtcaa agcgttttat
    19441 tatgccaaac gttccaaaag tttgcatgaa gatgtgatcg aagcggatcc tttgtattat
    19501 gacgcatacc ttagtatcgg tatgtataat tattatgcgg cggcggcacc gcgctggatg
    19561 gatgccatcg gttcgctatt tggattgagc ggagatcggg cgacgggtat tgcgcagttg
    19621 gaaaaaactt accggcacgg aaaaatattt aatcttgaag cgcaattttt tctcgccaat
    19681 gtgtatctcg aagaaggcga ttacgaaacg tcattgagga ttttttccca gttaatggat
    19741 cgctttcgca gtaacccgta tctgatcaat caagcgggca tggttagttt ttatttggaa
    19801 aactactccg tggcagagca aatttttcag caatccatac ttcagacatc gcaaactacg
    19861 cgttcggctg aaatgttggc ctcgtatttt ttaggccgga tggcacgctt gcgaggcgac
    19921 tacaccaaag cgattaccta ttttaataaa tccgttacga tcgccgatca atgcgtcctt
    19981 tttaaaatgg tagatggctg ggttgccggt tctgcgttgt attatttggc tgagacctac
    20041 gaattatccg gagatcgtac aaaggctgag atttggtacg aaaaagcgcg ggatgatgcg
    20101 cgcagcggca aaggtatcgt acaaggcgct aaaaatcgcc tgaaatatcc gatcggcgaa
    20161 tttgaaatgg agtttatcaa aatccgacag aaagcgctca tccagccgga accttcgcaa
    20221 ctgatcacgc tcaatacaat gctggcttcc gcttccaata aaccggaaat ggcaaagtat
    20281 gtttcgcaga ttcattatta tatcgggcgt acacaatttg cactggaaaa ttatccggaa
    20341 gcggagcgtc actttaccgc ttcgctcgat acacaaacgg atgacggtga agaaaaatgg
    20401 cgcacggcat ttaaccgcta ctatcgcggc gccgcacggg tcaagcttgg taaaaaaagt
    20461 gaagccaaat ccgatttgaa gcttctgacg gatgacgctt cgtatgttga cggaaatcgt
    20521 attcgttttc gtgctcagca gttgttgaaa ggattgtagc atggcgaaga ttcctcaaca
    20581 cacgatagat gaagtgaaaa atgcatggga cgtggtcgaa ctcgtatcgg gttatgtcgg
    20641 gctcaaaaag aaaggtcaca atttttgggg gttgtgtcct tttcacaacg aaaaaacgcc
    20701 ttcattttcc gttaattccg ataaacaaat ttttaaatgt ttcggttgcg gagccggcgg
    20761 tgatgtgatt tcgtttatca tgcaatacga aaaaacgagt tatccggaag ccatacgatt
    20821 cctcgccgag aaaaaaaata tcccgattcc gcaagaccat tctccggagg aaatcaatca
    20881 acgttcggaa atcgaagaat tatattttat aaacaaactg accgccgctt tttttgccga
    20941 gcaattgact ctacctcaag gcgatgaagc gcgtcagtat ttgcggaaac gcggttttga
    21001 tgatgccgtg atcgccgcgt acggtatcgg atatgcaccg gatcgttggg acggattgtt
    21061 cgagtatgcc aaaaaagaat cggtagatcc gcgcttactt gagcgcgccg ggttgatcat
    21121 tcctaagacg ggcggttatt atgatcgctt tcgccatcgc ctcatgtttc ccatcagcaa
    21181 tgtttccggg cgtgtgatcg catttggagg ccgccaaatg cgggatgaac cgaattcacc
    21241 gaaatatctt aattcgccgg aaagtgcgat ttatcataaa agcaaagtgc tgtatggctt
    21301 agcgcaagcc aaggacggca ttcgtcagat ggacgaagtg atcatcgtcg aaggttatgc
    21361 cgattgtata agtttgtttc aatttggtat tcataatgtt gcggcatcga gcggtacggc
    21421 atttacggca gagcaagcgc agctcattcg gcgatatacc gaaaatgtat gtctggtgta
    21481 cgacggcgat caggcaggca ttcgtgcggc cgaacgcggt ggcgctatca tgctggaagc
    21541 cggcctcaat gtcaaaatcg tcgttctgcc cggagaacat gatccggatt cctatgtgcg
    21601 tgaagccggt gaaagcgcga tgcaacagtt ggtgcaaaag gggcaagatt atatcaattt
    21661 tcgtgtagtc gaatggttga aatcggataa acttcgtacg gctaacgata aggccaatgc
    21721 ggtttcggag atggcgcaga tcattgcgcg gatggacgac agcctcaagc gtaatgcctt
    21781 catcgaagaa gtggcgcgca aattggatac cgcgttaccg ttggttgcaa aggcggtaca
    21841 aaaaaatcgc agcgccgtgg cacgccaaaa agataaagaa ccgatcgtca tttcgcatgc
    21901 tccggcgagt tcattgacta cggcgtcgtc atctgttccc gaaaaagttt tgcgtgcgga
    21961 gcgcggtatc ctgcgtgaac tgttgcttaa cccttcgcaa tgcgaatacg ttttcggtta
    22021 tgtcgaaccg cgcgatttca aaaatccggc catacaaacg ttgatcggaa aaatccactc
    22081 gctttatatg gaagaaacgc cttacgacta caatgtggtc gtacaggatt gtgaggaaaa
    22141 aatcaaaacg gcggccgaaa ggctgatttt cggagccggt ccgcgccagg ctatcgtgga
    22201 ggacaaatac gaactttccg aatgcattcg cgaaatgcag ttgtacgatt tacaattaga
    22261 ggaacagacg gtacgtgaag cgattcgtgt acgtgaattg gaaaaagaag accctgccga
    22321 actcatggct tcgcttcgac atatcgcgga tcgtatcaag gccgttcgtc aaagtaaact
    22381 catttcgctc tatgaaaccg gcgagcatac cgatgaggtg ccgttttgat ggatacgttt
    22441 aagtttgatg tggtgatcat cggtgccggg ccgaccgggc tatcctgcgc cgtcgaatgt
    22501 gcgcgtgccg gtttgtcgca tgtggtgctc gaaaaaggtt gtatcgtcaa ttcgatctat
    22561 cactttccct ccaacatgat tttttttacg accagtgatc ttttggaaat cggcggtgta
    22621 ccttttacta cgccgaatcc taaaccgaca cgggaagaag gacttcaata ttataaacgt
    22681 gtcgttcaat attattcttt gaatgttcgt accggcgaaa aagtgatttc gattcaaaat
    22741 ctatcaaacg aatttttggt ggacaccgaa tcgaatgatc aacagaagcg ttatactgcg
    22801 cggcatatcg tgattgcgac gggttattat gatcaaccca ataaattatt tgtaccgggc
    22861 gaagaatcgt cgaaggtatc gcattattat accgatccgc atccctttta tggaaaaaac
    22921 gtgttgatcg tcggtggtaa aaattcggct tgtatcgctg cattagaatt atatcgctac
    22981 ggagcaaaag tcaccgtcat ccatcggggc gaagaaataa aaaagagcgt gaaatactgg
    23041 atactaccgg attttatgaa tcgtgttcag gaaggtgcgc taacattaca tttgcagtcg
    23101 gtggtaacac atattgctga taacgaagtg acgattcaaa acgtgattac acgccaggaa
    23161 acaattatag ccaacgactt tgtattcgct ctgacgggat atcgcccgga tgaacattta
    23221 ttacgaatgt gtggtattca attggatgag cggctcgtac ctttgcataa tccggaaaca
    23281 ttggagacca atgttcgcgg catctatgtt gccggttcag tttcagccgg attggatacg
    23341 aataaattat ttattgaaaa cggacgtttt cacggtaaaa cgatcgtaga gcatattcga
    23401 tctaaagaat gagttgcatt tagctacgga atcaatgcat gtccttcggc taatacctga
    23461 tcgtaataaa tttcaaaagc cggattaaca gggcatatgg cactacaaaa agaactccgg
    23521 cggataaagc gactattgcg ttggacaagg ccgatatgct caacacgatt aaggcggggg
    23581 tcgcgtgtta tatttgaagg aactatgaaa cggctcaggt gcgttgatag cgaataagat
    23641 ggtcatcgaa tccatgcgcc aattttctga agcgtgcaaa ttttcagaag ttgggaaaag
    23701 aagtgagatg gggaaatggt atgtttttct aaattaggga aattaaattc ggtactgagg
    23761 cgaaaaagag agaacaatat cagtaggttt ccagcaaacc gactaagacc ggatatacta
    23821 agttacactc taacaatagt tttttatttc tttttcgtct ttttattgag agggatcaat
    23881 gtcagctttt tcaaagatta gatatactgc atataatgag attgattttt acaaattgag
    23941 ggaaaacatg aatagttaca cggctttctt attaatttta ctttttccgg ctaaaattaa
    24001 tagtcaaaat ctaagagaac atgattctct acgtattgta cgtttattcc aatctgctga
    24061 aaattcaaat aagtcaggga atattgacaa cgccctattt tataatagat acgctgcgag
    24121 tttggcctta aaatataaac tttggaatca gtactctact gctgttgccc agacgacaaa
    24181 gaacttaatc caaaaaaaca aagctaaaga tgctatatca gaatgtgata gcgcaattca
    24241 gcatattatt agaagtgaca ataaatcatt tggtaatatt tcacgattgc taaacaacaa
    24301 atcaatcgct ttgaggcgat tgtctgatta caatcaagcg cttgaaagtg taataaaagc
    24361 tgtttctttg ctccaagaaa ataatgtaga gccaaacaaa gaaatattag cagactgttt
    24421 tggctcaatg acaaatattt attatgatat gggtaaatac agagatgcca tgattgcaaa
    24481 tgagaatcaa cgtcgtattc tttcaagtat ttttggtaga aataatactc gtattgccgg
    24541 ttcttattcc aatcaaggac tcaattttca tgcaatagcc ttatacgatt cagcgataac
    24601 ttattttgat tccagtatat cgattcatga agcatgtggt aattcagaaa gtttggacta
    24661 tgcaagtgct ttgagcaata aggggatttc tttttttgaa aaaggggatt ttaaaaaagc
    24721 attattatta tacaatcaag cattggaatt acgaaaacgg cttgttggag ataagttttt
    24781 atatattgca ggtaattatg cgaatatagg tgtgacctat catgagttag gagattacga
    24841 tgcatctatt atttatatgg aaaaggcgtt gtccatatta aatcagctga acataacaaa
    24901 acatcctttt atcggaggta tttttgcaaa tatcggaatt agctaccgta gcaaaaaaga
    24961 ttatgaaacc gctgtcaagt atgtatttaa tgcactgagg atgcggcaat cgcttttcga
    25021 aatggcacac cctcacattg gagatagcta taacgatctt ggcaatattt attttgactt
    25081 gggcgactat aaatcggctt tgatctatta tgattctgca tcttatatca agtcacaaac
    25141 aacctcggct agttcacctc agatggcaaa actttatagc agcaaagcag cgctttattc
    25201 cattaccggg attttagaca gcgccaagca ttattataaa ttagcagcgg aatcttggaa
    25261 actgatttat ggtgaaaagc attacatgct ttctctttca ataatgcatt tagctgactt
    25321 agaagaatta aataacttag attcgacgat tcacatgtat caaacagcga tatgtactaa
    25381 tactctagat tttgacgata caaacgtttt taataatcct aatgtagaat cacaaatatt
    25441 atcatatgat gttatggttc aattgcttag taacaaagcc cgatatttgc ttaaaattta
    25501 cagcgaaaaa aaggatacga tgtatgttgt cggagcttat aatgcattgg aaattgcaca
    25561 tctattaata aatcagaaac aaagaacgat tattacggat aatgctaaat tttcaaatgt
    25621 gagtattgga caagacgtga tcgatgattt attagaaaca ctttacatgc tggatgaaat
    25681 tcgaggaaca aaaaatgctg cagaaatgta caaatacgtt gtaaaagcca agtctagtat
    25741 ttttatcgaa agtcttaaag atttagaaat tacaaaaggt gtcggcgatg ggaagctttt
    25801 aatgagggaa aatgatctca gagagcaggt ttctcaaaga gagattgaac tagccaaaaa
    25861 attagaatca aacaagtttg aaatgaatga tcatgttttg ggtatcaaga atgatttatt
    25921 tgatttaaaa gaagaacatg ctagcgtgat tagcgagcta aaaaaaacgt tcccaagtta
    25981 ctacaattta aaatacccat cttctgaaat tgatatttct gaaatccaac gcggactgaa
    26041 tgataacacg gccttaattg aatatttcat gggtaaaaaa aagtggtatg tgtttgttat
    26101 aactaatgac gattttgaag tttttcgatt ctcaaaagat actgtgttcg atccgaccat
    26161 cgatcaatat ttgaaatctg ttcgtacaat aaataagccg gaattcgttt cgtccagtta
    26221 cagtctatat aagaaattaa ttgctccgct cgacaaattg atagattcaa aaaagcattg
    26281 gataatcatt cctgatggga agctttattc cgtgcctttc gaatcgctga tctcagctgt
    26341 tcaaaacggt gagaaagatt tcacgaaatt gaagtatctt atccatcgtc acgatatacg
    26401 ttatcattat tcggcggcgt tatttttaag ggcttctgaa aataaaattg tatcacagag
    26461 tccaacattc cttggttttg cgcctatatt cagcgattcg ttaaaaaacg gtcatatttt
    26521 aaccacaaat acgaccgtcc ttgatacctt gagtggcatc aggtacagat ttgttacaga
    26581 cgatagtaaa gaattgatgc ctttgccgca ttctgaaacg gaagttgcaa atattgtttc
    26641 tttgtttgaa aaaaaagctt ggccagcacg ggcatatttt catcggcaag ccaccgaatc
    26701 taacttcaaa aatttctccg aagccggaaa gttcctgcat attgctacgc acggtcttat
    26761 taatctgaaa aaaccgaaac tctctgcact tttattttcg caacctcaga atacgaatca
    26821 aggagaaaat tcgatcaatc gaaatggcga gcctgttgat gatggagtat tgtattccgg
    26881 cgaaatatat aatctaaact taaaagcaga tttagttact ttaagtagtt gcgaatcggg
    26941 tatcggagaa tatataagag gcgaggggat gatgacacta acacgcggtt tcctttacgc
    27001 tggttcgaat aatattttat tttcgctctg gaaagtacct gatgaaagta cgagtaggtt
    27061 aatgacggag ttttatcgtc aatgtttgac gaacaaaaac tatagcgaat ctttacgtga
    27121 agcaaaactg ctgatgataa agaaacaagc tacctctttt ccactaaatt ggagcggttt
    27181 cgttttgata ggaagatagc tgtttcttaa gtcacattag cggttttttt atgataaatc
    27241 gccctatata taaaaggtca gttgaggttt ccagtttcca gtagtaaaga cctggcatta
    27301 cggtcgtggt gttccatata aaaggggcat ttattgaaat ccgttgcatt tgttcttctc
    27361 ggttattgag tattaccaat tctaaatttt tagtttcttt tggtttccaa tcaaaaatga
    27421 cagaatcgct gataattgcg tccactagtg gagaaattat ttgtaatgag tttttgtctc
    27481 tgaaattttg acgtatgata ttgtcgaaat ctgcatttgg ggtgtaattt tcaactaaga
    27541 aaccaatgtt cttttttggg ttaagcgttt tttcgtgtat ttctttgtcg ccgctatcgt
    27601 cttccttcac tttctttacg gtatcgatca aggcaattct tgaatcattg gaaaaattgc
    27661 cagatttttt ttctggaaaa aattgatata aagtaaaaaa gatcaaggca aatgaagccg
    27721 ctactagtat atatttgaaa attctgtctc gtttattgag tcctgacgcc acggcaaata
    27781 actctttccc acccaacttt atcgttgttt ttaatttctc cgtttcaagg agttcttcaa
    27841 aacattcact acaaataaaa taatgttctt caaaagacag gcgatctttc tcgctcaatt
    27901 cgttatgtaa ataacgatca atcaacaagc ttcgttggtt cttatatgag catgattcca
    27961 tttatttacc ttttaaattc ttttttaact tatccgttgc ataacggatt aaattaccta
    28021 cagttttttc agttgaacca atggctttgg ctatttcacc gtacgacaaa ccctcaaaat
    28081 aacgaagctt aagaacttcg cggtggttat tctttagctt agacaattcg ttcttaaatg
    28141 cttcaatatt gctctttatt tctaagtaat tgctgttatt atcctgtaat tcgtttacat
    28201 cgatgtcaac gaatttatct aggcggtttt tacgttgtcg aaagtaagtg ttgattttat
    28261 tttctactat ccgatgaata taggagccag catttcgcga agtgtcaaag ttgcctttgt
    28321 gaaggctatc gactatagca agatatactt ctgcaacaac atcatcaatt tcctcctgtc
    28381 ttttaattcg atacttcact ttacggcgta tctgctcctt gaaagcatca tatagctttg
    28441 aagcgagttc ctgaacattt tgctccgtca ttgtgcagtc agtgattgta gtgattactt
    28501 ccataggatg cctgaaaaat ataaccgtgc gattttacaa tacattctaa ctcatctaaa
    28561 aataaaaaaa aataataaaa ttcagcattt ctgcgtttgt agcgaatact tcttatggac
    28621 gactgtgtta tatcaatgtc cacatctata aatgatgtgt tccctgtata tgacacaaaa
    28681 tgatacgtaa aagaacaata tgagacttga tatactttag tatcaacccg taaattttag
    28741 agaatgagga gatagtcatt tcagggggca ctaaaatcaa agaagaatta ctgaaattga
    28801 ttcacagacg gctcaacgag cagcctggtt gtatcagtga attagccaaa caaatcggta
    28861 tcagccggag caccttgtat cgtttaaaaa acggtgatcc caaaattgaa gcaagggact
    28921 ttactattga caggatcatc cgtgcgctaa atatcagcta tgaaataaag aacgggaagg
    28981 tgatcgaagt ggaaaataga agattaattg cagtgagcca cgacccggat gaaatgagat
    29041 tatttaagga atgggtaatg aaaatagatc acattgtggc aaataatttg aatagtaatg
    29101 attacgaatt gattcgattg ttggtgtgcc gttgtctaaa gccatgaatg caaacacacc
    29161 gataaaaaag cacttttaat aaagcacttt taaatttctt caactttata aagaaaggta
    29221 tttttatgac aaaaagatgt gtgtctcatt tcctgcttgc aattttttta gttgcaacag
    29281 gttcttccca tgtttggact caaaaatttg aatgttttgt attgcagggc ccgcaaaaaa
    29341 acttaaccgg ggtgaaaaag atagcgctta tggattttat cgggaaaaac ggatccggag
    29401 taaaattagc tgattatttg acctcggaga tgcttgtcga aaatagagga atcagagata
    29461 tcgccggatt cttttcaaca aaagagggta aaaccttcat caaaggagta aaaacaaata
    29521 tttttacagt tgtagagcga tcaaagattc aacaattatt gaaagaacaa aattttagca
    29581 tgaccgatgc cgtagatgca aatcaagcgg ctcaagtcgg taaacttctt ggagttgacg
    29641 caattataac cgggagcgtg gattattctt ttaccgatac cccggaagaa tttaaatcaa
    29701 aaaagaagaa cggtagtgta aaaatcaatt ataagctgac acgtagtgta gccgtgtcag
    29761 caaaaatgaa aattatttca gtggcaaccg gtgaaatatt gggcaattca gataaagaat
    29821 ataaaaattc agatgcaaaa atggatgagg aacgatctgg tattaaatcg gttgaagcgc
    29881 ttgttgatga atgtttgaaa cccatggccg gagagctggt cacttatttt tctcctcagt
    29941 ttattttgaa taaatacaaa ttacagaaga tcaaagtcaa agagtttaaa aaacaagcgg
    30001 aagaggccgg agattttttt gaaaaaggag atctgaatcg tgcatatccg gtgtacaagg
    30061 ctgttgcaga tgccgatccg tacaacgctt ctgcgttgta taatttgggg cttatttatg
    30121 acatggtggg cgattttgaa caatcctacg catgtttgaa gaatgcttac gaactggagc
    30181 ccaaagaaga agattatcaa aaagcattcc agaggaccga aaaaaattta gagttttcaa
    30241 aggtgcttac ctcaatgggt atagtgccgg aaaagtacga tttcaaaatc gatgagacgg
    30301 ctttggccgc caaagtaatg atcaaaggcg ataaatcaga tcgtatcgaa gtgttttctg
    30361 agcccaaagc cggatcttca gtcgtggcaa aaattcctgg tgatagcgag tttgtggttt
    30421 tagaaaaaaa tggaggatgg gtaaaactaa aacttcttgg cggaaaagaa gggtgggtgc
    30481 aaaaagataa ggtaaaagaa tagagaaaaa agcatctatg cggtcattgg agaggattgg
    30541 ggattctata cgcatggtga cttaacaaat catccaatca gaaatgtttg gtaactgcgg
    30601 atatctctga tgtgttgtat ctattaagac taataatcaa aataaaattg taaaagtgaa
    30661 tatggcgtgc attgtttaga atagcataaa caaatgaggt tcaaaaatga aaagtttatc
    30721 tgtcacactt ctattcattt ttttgttgtc ttgtactgaa ttacaaaagc aatctctaga
    30781 tatcattcct gtaaaatcgg caaataaatg gggttatatt agtattgatg gcaaatttgc
    30841 tattaatcct caatttagtt tagcgactta ttttgttgat ggtatcgcca tggttaggtc
    30901 gattgaaaat aaggtcggtt atattggaaa agatggcaag tatattatca atcccgtcta
    30961 tgcgaaagga actgtctttt cggaagggct tgcgtgtgtg gcgcttgaca gtagtgggat
    31021 catgtatatc gataaatctg gtcaaataaa aattaatctc acaaacgctg aatttggtgg
    31081 aattttttcc gagggtttgg ctccagttgc tctagatgaa aagtatggat ttattgacca
    31141 aaccggaact atgaaaatca atccacagtt tgatgatgct cgttttttta aggaaggaat
    31201 ggcctctgtt gccattatca atgaaaagga gggtttactt tggggatttg taaacgccga
    31261 aggtaaaaca gtcataaatc cgcaattcaa agaagttggc tttttccaag atggattagc
    31321 ctgtatttca aacggagaaa aaatagggta tgtcgataag gctggtatat atacgatcaa
    31381 tcctcaattt gatattccaa attccggtaa tgcaagtttt gaaaaaataa cagcctttta
    31441 taacggaact gcctgttttc gtcagggacg tgaatggggt tttatagaca aacagggtaa
    31501 aatcattata aatcctcaat ttagcgatgt tagtatggct atagacgggg tcgcaccagt
    31561 tgcctcttca aacggaagtt ggggttatgt cgacaaagag ggaaaatatg ttatcaaccc
    31621 tcaattttct gatgccagcc cattttggaa tggaaatgcg tttgtcaagt caggtgggaa
    31681 gtacggaata atcggaaccg atggtaaatt tatagtcaat cctcaatttg atatgatatt
    31741 atggccatct tggacatggg tcaaaacatc caatagaatt gattttgtat gggtaaagtc
    31801 gccgatagaa ttacgatgga gagagataaa tgaaaggatt atgtcgctct acggacgtct
    31861 tttttattac ggtaatgcgg ccgtatcaga tcgaaaaaaa gtagatgatt ttttaaatga
    31921 agcggataaa gcagtgaaag aaaaaaatta ttttcaggta aaacaaaaat atcgcgaagc
    31981 cattgaattc atgaattcga taattgattc aaagcgtacc gataaaagga gtgggggaat
    32041 tgactgatga ggaaatttaa attcaatatt atttggcttt ggtgttttac tttattatca
    32101 gtattcaatc actatagagc tcatagtcaa tgcaacacat ggagtcctgt ttttgcttta
    32161 attgatgata aatcaaaatc acaaaatatt gatttccgag tagcagcgtc taaaagcgga
    32221 atatatgtta tactcgcgcc gggatccatt atttgggttt tcaatgatgc cggtagaaaa
    32281 atatcaacac gatcaggtaa aatacaatcc atagccgttt cagattccgg tcatattgta
    32341 attgctgaaa acttttcaat caaaatatca agagatctcg gaaaaacgtg gaatgatatt
    32401 tctaatgagc ctactccatt tgaaagaaat caaatttacc ctcacgagat atactatgtt
    32461 acaaggaata acggaaaaaa agttgaaggt gaaatcatcg tcaaaagcgt caagaattac
    32521 ggttttgagg caagtgttac gctatggtca acgagggatg gcaacaactg gtcgtccgaa
    32581 actaacccgg gtttcccctc gtttcgatcc gccaaacatt ggatttcaac acgacttgga
    32641 aagaagctat atctgagtga taatggaaac gatgtttttg attgtgaaga ttcaaaaagc
    32701 caattgacct cattatttac ttcagaagaa tatattaaaa gtttggaatt cataggtaat
    32761 aaaagtctgc tgattgcaac aaagaacaga ctttatttca agaatgattt ctctaaaaag
    32821 tcctcagaat ggattgattt atcagaaagc tttcccaaaa cctctgaaat aagcgattta
    32881 gccacaactg acagtttaat aattatgact tctgcgggtg ttgtttatgt ttccacaaag
    32941 gaaaaactaa tatctggatc aaattcccat aaatggtctg agttaaaaga aaccataaat
    33001 ggcggtatgg actggtcggg tggacagatt gagacacaaa agagttttga gaatatcaca
    33061 gcattttata aatctggtaa tattattgcg atttctgaat ggacttctat agatattgca
    33121 gtattaattt ctacagataa tggccaaagg tggaaaacat cgacaataaa agggaaaaaa
    33181 aggaagaatc aagaaaagtt ctatgtgata tttgaaaaag tcatagatat ttacatatca
    33241 ggaaaacaaa tttttgtatt aactgatgaa gaaggtcata gtgaaggtga ttatgttcct
    33301 tcaggacggc tatataaatc agttgatttt ggagcttcgt gggagacagt tcaagagaaa
    33361 atacgttatc caagaaatct ttactatgtg gatgaccaat tattcattgt tggcggaaaa
    33421 gatatttatg tctcaaacga tgagggaatt actctaaaga gttatagtct tggcctttca
    33481 gaactaagct gggttagagg gagtagtgtt ttcgattcat ccatttggat tgcaacaaac
    33541 aaatcaataa tgaaaagtgc atttcgcaat attcaattta atgtgataag ctctaagttc
    33601 acgggagaag taaatattgc tgatttttat cctggtgaaa aaatgtttag cgccattaca
    33661 tcgaacggat tagtttttca ctcgaccgat aaaggaaaaa cttggaagaa attgtcttct
    33721 atttgcgaat tacgacctgc ttcaacatat cgaattatag aatgcgatag cacattgttt
    33781 gtccttgggg tgagcattgt tcgttatgat aatgaaaaca aatcatgggt tacaatttca
    33841 tcaagtttac cctttaaaat ttggtcagga tggaaagctt ttgataatga gccttacgta
    33901 tttggtgagg gaggtttgtg gagactaaag ttaaataatt agttggattc tggaaccaaa
    33961 cgaatataat tattcctcta ttctttggtt taggagcgat agcaattggc ctgcctgttt
    34021 ctcttattat ggaatctttg aagtgggctt caactaaaat agaatcactc aagtatatct
    34081 taggggttat taccctcctc atactttttc ctctgattat tctatgttta accgatccgt
    34141 tagtgtttgt attacataaa ttttcaagta aacttattcc acttgaaaag tatattcagt
    34201 gatagcagta ctatggtttt cacacgatct gtcgtcggca acgggataac agccgttatt
    34261 agtggtgatt cgttaaggat tacaaaaaac agattcatct ggcctttatt cagtgatagt
    34321 catcgctacg gagcaaaagt caccgtcatc catcggggcg aagaaataaa aaagagcgtg
    34381 aaatactgga tactaccgga ttttatgaat cgtgttcagg aaggtgcgct aacattacat
    34441 ttgcagtcgg tggtaacaca tattgctgat aacgaagtga cgattcaaaa cgtgattaca
    34501 cgccaggaaa caattatagc caacgacttt gtattcgctc tgacgggata tcgcccggat
    34561 gaacatttat tacgaatgtg tggtattcaa ttggatgagc ggctcgtacc tttgcataat
    34621 ccggaaacat tggagaccaa tgttcgcggc atctatgttg ccggttcagt ttcagccgga
    34681 ttggatacga ataaattatt tattgaaaac ggacgttttc acggtaaaac gatcgtagag
    34741 catattcgat ctaaagaatg agttgcattt agctacggaa ttaatgcgtg accttcggct
    34801 aatacctgat cgtagaacaa ttctagcgca ggattgacac ctcgcaatcc cgtcaccact
    34861 ttttcagtcc aatcataata ttctttacgc cgcagcgtat cccatcctcg tggcggcgaa
    34921 tgcgtcacat cgcgtacgtt gcaaatcttg tcggcaaggc gtatgacctt agcggcaggg
    34981 ctcaatgcgg cagcggtagc aatctgcttt tgtttacgtt cgctcttcat taacgacgga
    35041 tcgtcggtta cttctaatac catagtaaga acagtctttc cgaataatcc gaggatttca
    35101 tcgggtgtgg tttgtgtatc ctctatggta tcatgtaaaa ttgcggccac gataatatcg
    35161 ggatcgcgta cttcagctac acgccaaagt aactctgcaa cctgaatcgg atgattgata
    35221 tacggcgacg cagcaggatc tttgcgtcgc tggtgccggt gtttttcagc ggaaaaatga
    35281 atcgcaccca acatcagcgc catgtccgtc tcagcataga tcataattaa cctttcagaa
    35341 acgcttctga tatcgcgtaa taaacggcca tgagtccgct aaaaatgctg ctcgcaataa
    35401 aacgccgttc gagaggaatc atgttttgta tgatgtagtt accggcaaaa gaaatcacga
    35461 tcgcgccggt aaaagaagat aagccgctta tccaggaagt gtgtagtaaa accgataacg
    35521 aaggccaagt cagaatggtt gggggattga ggctcgctcg aataagaaaa atatgccggg
    35581 cgatccaaag tggattaaaa tatgcgatag cggcgagcgt gcgtgggaac caatggttga
    35641 atgttcggtt gatccatccg aaatataatg ggatctcaaa cgcgtagatc aaaccgccgg
    35701 ccagcgccat cgtcacaata cgtactccgt taacctcacc ccatatgatc tgtgctatca
    35761 gatcacccaa aggatatatg ataaacccgg ctatacggtt cttatcgaga aatgtgcgca
    35821 ttgttcaggg tggtcgccgt agcttattgg atttcgttta gcaatgtgcg gattttagtc
    35881 atcagaatat ccacaccgac gacattttct acaccgtgcg ggatgataat atcggcataa
    35941 cgtttggaag gttcgacaaa ttgtaagtgc atggggcgca caaatttttc atactgctca
    36001 atgacggaat caatcgtacg tgcacgctca cgtatatctc tctgtaaacg gcgaatcagt
    36061 cgcacatccg cgtcggtatc aatatagact ttgatatcca tcagatcgcg taattcagga
    36121 ttttctaaaa tcaaaatacc ttccagtaag atcactttgt gcggttcgag agcagtcact
    36181 tccgaagtgc gtgcatgagt tttatggtcg tagttgggaa tgcgaatggt ttcgccgcgc
    36241 agcgcttgtt taagatgatc aaccaacaaa tgattatcga aagcgtcggg atgatcaaaa
    36301 ttatgaccgg cgcgttcctg atgcgggatt tgcgataaat ctttgtaata cgaatcctga
    36361 gcgatgatga ctacttgatt atgaccgcca agggtttcaa agattttttt ggatacggat
    36421 gttttcccgg aagcagttcc tccggcgacg ccgatcaaga tgtgtttttt ggacataggt
    36481 gtacgttact gaggttttat tttgggtttc aatttttcga tggctgttaa tacggcttca
    36541 ttcgtagccg gtaatgtaaa ctccggttcg tagcgatgat ggcttacggc cgacaccgca
    36601 tctttgatcg cgagccagcc tgaaagacct agcataaaag gcggttcggc taccgctttg
    36661 ctggaatgta tcgtattggc gttgggcgca ttttgtaaaa gcgcaacgcg aaaatctttg
    36721 ggaatatcct gcacgccggg aattttatac gtatcgggag aatgggtgag taaacgaccc
    36781 gccgcatcgt gtttgatttc ttcggtagtg caccaaccga ggccttgaat ataaccgcct
    36841 tcgatctggc ctatgtcgat cgcaggattg atggattcgc cgacgtcgtg caatatatcc
    36901 gtgcgcaaaa atgaatactc gccggtgagc gtgtcaacca acacttcagt aacgcacata
    36961 ccgtaagcaa aataatgaaa aggtcgtcct tcgccttttt ctctgtcaaa aaatattccc
    37021 ggtgtgcgat aataacctgt agcactgagg ctgacttgtt tcataaccgt gatcggcatg
    37081 agttcgtcaa attttataag gcgcttggga taacgcatat cataaaccgt atcatcctca
    37141 aatacgatat catccggcga tgaacgcact tcgagggcgc gctcattaaa ataatctgcg
    37201 gcgacacgca tcaatcgtaa ttttaattga tcaatcgcat ttttgacagc catgccgttg
    37261 agatccgttc cggcggaagc cgctgtggcg gaggtattcg gaactttaga tgtattggtc
    37321 gcgttgactt tgatgcgggt gagaggcacg ccgagttctt tggccgcaat ttgagcaatt
    37381 ttcgtgtgga gtccctgacc catttccgtg ccgccgtggt tgacgagcac gctgccgtct
    37441 ttgtagatat gaaccagtgc gccggcttga ttgagaaaag aagtcgtaaa tgaaat
//
LOCUS       DAOQGF010000020        36708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_501_length_36708_cov_6.817587, whole genome shotgun sequence.
ACCESSION   DAOQGF010000020 DAOQGF010000000
VERSION     DAOQGF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 36708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_36708_cov_6.817587"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1627)
                     /locus_tag="PK937_04775"
     CDS             complement(152..1627)
                     /locus_tag="PK937_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNH31914.1"
                     /translation="MKRSQKKLLFLGTFLCVVILLIPESELKIDTTSFDHSFEWNQDS
                     LWQTLEREFVASRYEEGLQTVKLIQVSMAQLQRMTDTLNTTTVNTSDELLWRIERSVY
                     YLATRVAVKPEFVADFSRIIMNLRFVVKKQSRNWDMKDRITRERLYRILYGMRTALEE
                     VLLQSQTDKFAEINPGHAESSVTPHADVLGVMLHSGDILVSRGGAPTSALIARGNDFP
                     GNFSHVALVHIDSVTGKISLIEAHIEKGVAIATWNEYIADKKLRILILRPRHDIPVLA
                     SDPMAPHKAADFALQLALQKHIPYDFVMNYRDHGALFCSEVASSAYESVGISLWMGIS
                     RITSPGTKQWLSYFGVRHFETQEPADLEYDPQLQVVAEWRNASALLQDQLDNAVIDIM
                     LEDADAGKPIEYNMWYLPAARLAKTYSVLLNFFGKVGPIPEGMSATAALRNQWFSERH
                     HAIRAILAEEVKKFREKKQYFPPLWERHRLAREAKNKLDMN"
     gene            1947..2933
                     /locus_tag="PK937_04780"
     CDS             1947..2933
                     /locus_tag="PK937_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31915.1"
                     /translation="MIVLTDAYLNPVWYKAIGSEKADYGTDITETSDGNFLAVGTIQI
                     TGNNKDVVTLLLDRSGKLLASKSYGGLAPDEAQRLIKLDYGVFAMIGNTGSFGHGASD
                     VWLILLDAFGDSTRSYTFGTEDDEHVLGAIQSQNGSIYCTGYVSAAEYVPFLLTINSD
                     MSNHLWVRYPARNSRDEAYDIKETSNGDLIMVGRTNIFRSPYENYDAQVFKTDASGAK
                     SWVTRFGKTKYDILESCIPLDDNGCIVIGSSLHVSPKYVANQQALIARIQSTGDTVWT
                     RQIGGSPPDEAVCILPIGLNRFLIGGNTRNGQYGDWDIVFFMIDENGNHDFN"
     gene            complement(2984..3745)
                     /locus_tag="PK937_04785"
     CDS             complement(2984..3745)
                     /locus_tag="PK937_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH31916.1"
                     /translation="MLIDDESLARQRVRDMLSVSFAGQIAIMDEASDGIEALEKIESL
                     QPDFIILDIQMPGLSGFEVLMHLKKVPPVIFATAYDHYAIRAFDVNAVDYLLKPFDEE
                     RLKRAIERVIAHLTVPESANTLSIQKLITDVMQEKNTQTAYLERIPYRDGEKIRFIKA
                     MQCLWFDTQDSVTYLHTDQSAHDIRYTLDEIEQRLRPSEFLRVHRATIVNLNAIRELT
                     PWFNGAYKIILNDKKNSEIDVARGRVQALRDVLGW"
     gene            complement(3761..4846)
                     /locus_tag="PK937_04790"
     CDS             complement(3761..4846)
                     /locus_tag="PK937_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH31917.1"
                     /translation="MKFNDTIKRFIIHEIAWAFVVICTTGFIIGALYVPVWTWNNYWI
                     SLRVSFSFYVVMRFLMGTFAERVIRRFVPRTLERPKKFFAIEILIRVILGTVGSFLAF
                     MLAEIITGINFLISLRMIYVQVFIAVILTLLITVSFYSVAFYREMLNKMKIAQELREA
                     AVEAELRALRAQVNPHFLFNTLNAIAALIASDPKRAEMMVQRLSEVFRYVLVASEKET
                     VTLDEELTFLDNYLRIERVRFGEKLTICENVDVTVRPVRVPGLILQPLVENAIKHGLA
                     PRRENGLLEISASRQDDFLILRISDDGEGFSVDRNGKVIFNREGVGVKNVRERIEKMY
                     GGKGQLILSNRPQGGAVAEVRIPIRVS"
     gene            complement(4862..5956)
                     /locus_tag="PK937_04795"
     CDS             complement(4862..5956)
                     /locus_tag="PK937_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31918.1"
                     /translation="MMNQPEPYSYFHALRVYVITSLRAISRNPSSIVFGFIFPVIFIV
                     IFGFIGNRTLNLTIGVIQNDSQENPMMEMLSHMDQIKLMRNPDDVALTEKLRKGEVDA
                     LMRVEKTDSGYVAHIQTSDASPNGIVVLNMMRGLTAQANLLMMQVQHVPLAIAHTTIE
                     GRRYKTIDFILPGQLGFVVLSSGIFGAAYLLIALKSRLVIKRMFATPAPKSVILVGEA
                     IARMLFALAQVSIIVLIGKWVFGFTLINGWVTLLMMLLVCLIALMVFLGFGMIVSNIA
                     RHEQAVPPIANLIVMPQFLLAGTFFSIRVFPEWLQPVCHALPLTYVNDALRKIAFEGA
                     GFADVGFELMVLGVWGVIVYAVAVKLFKWE"
     gene            complement(5977..6744)
                     /locus_tag="PK937_04800"
     CDS             complement(5977..6744)
                     /locus_tag="PK937_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31919.1"
                     /translation="MSERKKIVQVRDLKKNYGSFEAVRGISFDVYEGEIFGLLGPNGA
                     GKTTTLEIMETLREKTSGEVLIEEFSVDRDPMPIKRRIGVQLQEAGYYPNSTLLEVCA
                     LFAGMYNVHHDASALLAKVGLTDKAKTRVDKLSGGQKQRFSIATTLIHKPRVVFLDEP
                     TVGLDPQARRNLWELIRSIRDDGTTVIMTTHYLDEAELLCDRVGIMDEGRIIALDSPE
                     RLIHGLLATGFQRPTRVREATLEDVFIQLTGKELRDA"
     gene            complement(6828..>8442)
                     /locus_tag="PK937_04805"
     CDS             complement(6828..>8442)
                     /locus_tag="PK937_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH31920.1"
                     /translation="FTFVKTAVIRKLYFSSGGFSVRYGNALSGVLDIETDNHVAQNRI
                     ALDANLVSVGGGLQRQIIPNTLSAQVYADHTSTALLFKMNKPAFDVVRDPVTSNVTGI
                     VNYNYRPSGQIQAMILESRDAQTFDLTLQSNSQRYALETGNRVMSLQWSELFNPQWTS
                     KASWSYSMYRNDWTFGDWRRANREYNIKWRWDHHYALLNRTILSFGGEAYGDFYRFDY
                     ILPLRRGEYYNGADSVALQGNHHATVVAAYAEVQQKITRRWSAQIGFRTDYQTLSQNQ
                     VYDIRGSVVHEFAANSFLRFSAGTFHQFPHITMYDKTIGNPDLKAMRARHAVVGWEKN
                     AATYQLKIESYYKWYDHLPLSDEDKNYVSQGRGYARGLDFFLKGGFAKTSGWFSYSFI
                     QTRRKEMQIQTMRPSLYDITHNISLIVKQNLGRNFELASTLRYATGRPYTPVTAGQYD
                     PVRGIWQPVYAADYSDRFPDFKRLDARLSKFFILGPQKHVVLYVEALNLLGIRNILDY
                     SYSEDFSERHSVPSYFSNRTVVMGFSLSL"
     assembly_gap    8443..8499
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8500..9068)
                     /locus_tag="PK937_04810"
     CDS             complement(<8500..9068)
                     /locus_tag="PK937_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNH31921.1"
                     /translation="MKKTFIVVLLLCAQYQSYAQTAAPYTVRVQLVDSDGKKVAYANV
                     YFADGDFAGAFTNDSGLAIIRTHIAGERHLTASLIGYDTEVKKIKVPHAQTMHVTMHE
                     KSVELGELVIEASAYGGGEGKVAVSKIDVYTTPGGAADVFQSVKVLPGVTQTDETAAV
                     PVRGGSPSENLILFNGATLAHPYHGENTAG"
     gene            complement(9085..9861)
                     /locus_tag="PK937_04815"
     CDS             complement(9085..9861)
                     /locus_tag="PK937_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31922.1"
                     /translation="MKRMFVVMMLTAGTLFSQNPAVSIAIEGKNKIQNGFAAWNETMI
                     REGSALCERAAMADPQNVWPLYWSAFAYYRLAIMKLYGGEPDEDAGKQYIETGLSLLE
                     KANTLKPGHAESLALTGSLTGMKIRFNPLSAMWLGPKSQEYMSKAVEADPKSPRVFYL
                     IGIGTMNTPSMFGGGADKATGDFLKAIRLYEEEAANPPADPLAPTWGYDECYSFLGSA
                     YEAQKMFNEAKSCYQKSLTINPESRRAKAQLMRLENKNTQ"
     gene            complement(9891..10598)
                     /locus_tag="PK937_04820"
     CDS             complement(9891..10598)
                     /locus_tag="PK937_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31923.1"
                     /translation="MRYRVWLLVCIILITVSPGSAQQVRKTYGGFGGFIFGASKQDMT
                     GFNKTLKNSGYRTYENQSWMYGGGGFAVLGRFLFGGESHALMARNTVSENALYRVKLR
                     GAVGQFKIGYILLQKGSIRFYPFVGLGDSDFILEIAERIPADFEDILDQPARASRLRV
                     KATTIDAGWGVLHTLFDSGRRSGGGLAIGMQIGYRYTPAKNDWQRFGGDTEGRSIPLW
                     NGPYATLLFGAAGFGVR"
     gene            11017..11301
                     /locus_tag="PK937_04825"
     CDS             11017..11301
                     /locus_tag="PK937_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNH31924.1"
                     /translation="MALDALGMVETKGLVGAIEAADAMVKAAKVELIGKEKIGGGYVT
                     VMVRGDVGAVKAATDAGAAAAEKVGELVSVHVIPRPHPEVELILPKKGSN"
     gene            11320..11652
                     /locus_tag="PK937_04830"
     CDS             11320..11652
                     /locus_tag="PK937_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNH31925.1"
                     /translation="MADERALGMVETLGLVGAIEAADAMIKASNVKLIGKEITDGAMI
                     TIKVVGEVGAVQASVSAGEAAARRVGQVVSVHIIPRPDAMTESIIYDEGLVEAPSGGT
                     GSKKKLNA"
     gene            12008..13420
                     /locus_tag="PK937_04835"
     CDS             12008..13420
                     /locus_tag="PK937_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH31926.1"
                     /translation="MMFPLMIAAIGVFEITIRWFDIHAIEYAHIWIILIPLIFSIIIS
                     YLSARFTAKEVAHPIKLFIESAKAIARGNFDHKVNVHTSEEMTQLSRIFNYMTRELKE
                     INQGNIRKTIQEKIKTEAIIRNIADGIIVVGPLNEVLMINEVVEGWFHVQQKAVIGFS
                     LNFFFPELKFLVKQALDTQGETVFQDELTIKPLNSPSEIYLGANASKVMDNDDLIALV
                     IVLRNITKEKEIDRMKTELVNVVAHELRSPLTSIAGFSEIIKDPHLSEAIRKEYIDII
                     HYESGRLAEMISKFLDISRIESGKTVINKVPCDLTTVVDNTITANTPMAQKRSMHVSS
                     TLPKWVPLVHADPDLIGQVVLNFFSNAVKYSPEGSEIKMRVAVADDTVIVEVSDNGYG
                     ISKENMKHLFQKFFRAKDEKHVKDVEGTGLGLAFVKEIIHQHNGTIHVESEVGKGSSF
                     SFALPIDFTLPENAALKPAS"
     gene            complement(13429..15225)
                     /locus_tag="PK937_04840"
     CDS             complement(13429..15225)
                     /locus_tag="PK937_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31927.1"
                     /translation="MQTQDDDIMGKAYDARLMRRLLSLVRPYRWMAVVSVILLIAAQG
                     FSAYRPKLVQEAVDAAILAGDGLRLRNFAILFVVLLIGEFLLQYCVIYVTQLMGQRII
                     FDLRMKLFTHLEHLHLQFFDRNPVGRLITRVTSDIESLNDMFTSGLVYLFGDIFLLAG
                     IIVMMFVLDVHLTLVTLAILPLIFYISVVFKSKVRVAFREVRLKIAALNSYLQENITG
                     VTTVQIFNREKKNFEKFDALNRTLTDSHLDSVFHYAWFYPAINVASSFAIGMLIWYGS
                     GEVRSSSITLGTLIAFIQYGMLFFRPIQDLSDKYNILQTAMASSERVFKLIDTPTEIP
                     NPAQPITPKVMSGRIEFRNVYFAYNPQDIRSDEDYILKDISFVTQPGQSVALVGATGS
                     GKSTIINLASRFYEAQRGDILLDQTDIRRMDQYALRRTMAVVLQDVFLFSGTILDNIR
                     LGRHDISEAQVEEAARRVGADDFIRRLPLQYLEPVQERGSTLSVGQRQLIAMARALVF
                     DPRILILDEATSSIDTETERLIQKAIAQLMQGRTSVIIAHRLSTIRHVDQILVLHKGR
                     IRERGTHEELLAMGGLYYRLYQLQYKDQEFIA"
     gene            complement(15232..16551)
                     /locus_tag="PK937_04845"
     CDS             complement(15232..16551)
                     /locus_tag="PK937_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH31928.1"
                     /translation="MSEFNPLSNFVGSVSHTLSNRWTLHLRQSTHTPFVVLRLRFPVG
                     IAHEPADQPGLCKLLALTLKSGTSKQTALSIADALEYLAASLSVSVTHDAFTLHLTVS
                     REQLEPALLIVHEILTDAVFPEHELQRARGQMKADCKKKLDMPGVLLGELFQSTLFGR
                     HPYGRSTDGMESGVDDLSRDGALRFYNEKLTACGAIVTVVGDVDENKMRTFVENNWAS
                     HFKNSIDINAIHMDSPQPLGQRIFCLTDRPIAQAHIALGHTGPARGEPGHVPQMVLNY
                     ILGGAGLSSRLTHHIRTQQGLAYSVYSSLTKRKFGGMFSVVLQTSAAHAPRAVTAIVK
                     ELEAIKADGVSDQELEDAKLYFSGHHPFTMETIERMALLIEQGAFFDLPPDYVERELA
                     ELQSLEQDDLRKAADMIDPKHWVLAIIGNEALIRPPIQKLLHHYERQ"
     gene            complement(16554..17171)
                     /locus_tag="PK937_04850"
     CDS             complement(16554..17171)
                     /locus_tag="PK937_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNH31929.1"
                     /translation="MQRKVRADDRPKESTQTLDQTSATRTQDPKPEPTQLTMTGIHDP
                     KWDKILNYMIGGLGLVVLILVISLVIRINSSGTSEAETQTNEVPAETSPSTTFDGTQS
                     RPQKIRIEVLNGSGVPKLAAKAADYLRSKGFDVVSTGNAPNSNYKKTIVQDRAGNLAG
                     AQQVAVALGVGESGIIQQKNPQLMLEVTVIVGQDYKSLKFTNPRD"
     gene            complement(17223..17732)
                     /locus_tag="PK937_04855"
     CDS             complement(17223..17732)
                     /locus_tag="PK937_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31930.1"
                     /translation="MKKQTIFLLIFGLCSVSLSAQLKTQTGREPIDVHGGITSAVPGS
                     LLSFIGSDRVTMTQSYAMTYYSGGGASGTLGMYSNSLNIRLSDPLVLRLNTGLMHQSF
                     GQNKNSLMQKNREILHGAELVYRPSRHFQMQVGYSNNPYYTGNSLFNPGPQRSGSGGL
                     FDDYGFMRP"
     gene            complement(17808..18551)
                     /locus_tag="PK937_04860"
     CDS             complement(17808..18551)
                     /locus_tag="PK937_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31931.1"
                     /translation="MVKFVGSLIFLFSVVGCASRHVLIEPESPIVFSKRARGGAASWT
                     GEELNAPSLTIYANGVVIRPAYNEGKRRWMLGHMKPQVFRERIKHIQNIRDSVKAMHR
                     KTADTSAAIPVIEWITGSDTLAVRGLGFSVQGGWLDSMHYYNQEMDTWEYEALRPYVA
                     SRIRLYVKKSGSARGAQWPVWPVRAVLPSSVYTRDVSYYEPNVTENSVVIEGVIAREV
                     QSAIGQTGVYEKFIADGMVYGIGYVIEVP"
     gene            complement(18544..19245)
                     /locus_tag="PK937_04865"
     CDS             complement(18544..19245)
                     /locus_tag="PK937_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNH31932.1"
                     /translation="MVKPDIHLRAIIFDLDNTLVDFMRLKERAIASAAEAMIDAGLEV
                     KKEDIISKIWTIYNSQGYEYQKVFDIFLENALGRIDHKILANGIVAYRQTREASLVTY
                     PHVLSTLNELVKRRFKLAVVSDAPTREAWLRLCYLRLHHTFDAVVTYEDTFQRKPNPE
                     PFRKALTQMDIPPEHALMVGDWAERDMAGAKQIGMKTAFARYGDTFDTRESGADYELT
                     DFRQLLDIAVEPFHG"
     gene            complement(19257..20498)
                     /gene="rodA"
                     /locus_tag="PK937_04870"
     CDS             complement(19257..20498)
                     /gene="rodA"
                     /locus_tag="PK937_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNH31933.1"
                     /translation="MVQRIKKFLQDLDYLILAPALMLCGLGLACIYSATFTSDQLNFT
                     KQLTFFFIGVVLMLMTVLIPLRLIDGASFTVFAVTIALLILVFFAGSVRYGARRWFTL
                     GPFGFQPSELAKIAAILFIARILALRQVNSSQVMNIIIVFSVAAIPMLLVMKQPDLGT
                     ALCVGSVALPILYWNGISLFSIFVMFSPIATVLVHIISGYSFNAFITMLFIILTILYF
                     SKRKTLIILGVFAVNVIVGLSSEPIWESLKDYQKQRILNFIEPERDMQGSGYQVTQSK
                     IAIGSGGLVGKGYLHGTQTQLNFLPEQHTDFIFSVIGEEFGFLGSLFLLALFGLLIFR
                     IVIIADAVDDKFASLALIGIASLFAFQLLVNIGMTTGLMPVTGIPLPLLSYGGSALWA
                     NMFLIGFVLNVAMRKKNYEIH"
     gene            complement(20482..22356)
                     /gene="mrdA"
                     /locus_tag="PK937_04875"
     CDS             complement(20482..22356)
                     /gene="mrdA"
                     /locus_tag="PK937_04875"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNH31934.1"
                     /translation="MNHHRVVTMYVSLAAILLMLLGRLFYLQIMAYDNFHARAEDNST
                     RTIDQIPSRGLIYDRYGRRIVENRPTFALTITLSEFDTLSTGEMQYVRSLTGMTDEDI
                     LKKLRGAWPYAPVVIKPEVDFVTIAHLEEERRFHRGIGYKVDINRIYADDITMPHLIG
                     YMSQITDNTIGYYKTAEFQDTDYKIGERVGVSGLEKFYEVELRGEKGYHRLETNAKGQ
                     VLRDLGISKHPRDGYNLYLSIDNDYQKFVESALGTRRGSIMVADTRTGEILAATSKPD
                     YDFRWFVDGITIDQWRELNENPDKPLFNRMVQSGYAPGSTFKMITALAGLEEGLVTPE
                     TYVECTGVYQLGGNKFRCWNLKGHGHMNVSSSLVRSCDVYFYELAWKLGINRLAKYAR
                     MFGLGEPTGTDIPNERRGLIPTTEYYNSRYGFNNWKGGAVVNLGIGQGEILVTPVQLL
                     QYAMIFANAGTYQTPHLVRYAEDSEGIRRVTFPVKHVAVKQEHMDMMRQAMFGVVNYF
                     EGTGKKAGLKAAQVAGKTGTAENVHGKVGSGTEGYHGWFVGFAPYENPEIAIVVLMEN
                     GGEGSDVAAPMSALVMNYYFNFVRNKKSFDPKEKDRKETPKNTTAPQAAVVEVHGTTN
                     "
     gene            complement(22363..22887)
                     /gene="mreD"
                     /locus_tag="PK937_04880"
     CDS             complement(22363..22887)
                     /gene="mreD"
                     /locus_tag="PK937_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNH31935.1"
                     /translation="MRRKRNILTFSLLGAAAFFYLMQVSFVSVFLAVGDNVPDLLLIL
                     TVFVSMRLGQIPGMLFGFTVGLWQDAMIDFYGLNALCKTIAAFATYFFAAERVMLIEK
                     YYFPIMIFFLSVIHAVFFYAIQSLDGVVDFWSLMFHYGLWNSLYSAVTAFVIYLSIPQ
                     RTLDYVRFNTIYDF"
     gene            complement(22891..23757)
                     /gene="mreC"
                     /locus_tag="PK937_04885"
     CDS             complement(22891..23757)
                     /gene="mreC"
                     /locus_tag="PK937_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNH31936.1"
                     /translation="MLWKLRNFYAGLKEILYVYRHSFVFLVLVIASILLLQNNPNPSM
                     IFLRKRVIYLAAMLGEKISSGPRWRDSDAVIKELQQRNIELASRNIVLEDAYMENVRL
                     RQLVEFRSRFPLSVRPARIIARESRPAISNIVLDKGSADGIRLHQNVINEYGLVGQIA
                     EVFEEHSVCQMLLDKNFRVASKIQRTRANGVVFWPGNPDEVAFYGVLKNLDVRIGDVI
                     LTSEYSEYFLPNFRVGVVTEVNNEIEGLFKDIRVQTAVDFDRLEEVFVVTDTSRTRSV
                     AVGFENYFIVKR"
     gene            complement(23797..24822)
                     /locus_tag="PK937_04890"
     CDS             complement(23797..24822)
                     /locus_tag="PK937_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNH31937.1"
                     /translation="MSLFDIFNNEIAIDLGTANTLVYVKDKGVVINEPSIVAINKRSN
                     KMMAIGKEAKEMEGRTHDELLTVRPLKDGVIADFEMTELMLQEFIRKVSPARFMGKRV
                     VVGVPSGITMVEQRAVRDSAEHAGAKEVYLVAEPMAAAIGVGIDIEASKGSMVIDIGG
                     GTAEIAVIALSGIVCDRSIRSAGDELTKSIITYFRVQHNLLLGEQMAEDIKCNIGSAV
                     PLAQELKMEIKGRDIVSGIPKTMKTDSEEVRRALEGTIREIVEGTKSTLEKTPPELAS
                     DLIDRGIVLTGGGALIKGLDERMRKETGLPVIVAEDPLTAVVRGVGRVLENLTKYQAV
                     ILKNRKY"
     gene            complement(24856..26409)
                     /gene="purH"
                     /locus_tag="PK937_04895"
     CDS             complement(24856..26409)
                     /gene="purH"
                     /locus_tag="PK937_04895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNH31938.1"
                     /translation="MIRRALISVSDKTGIQSLAQKLHQAGVEIISTGGTASALSQNGI
                     PVVKISDLTQFPEMMDGRVKTLHPKIHGGLLALRDNATHMEEANRHGIGMIDLLAVNL
                     YPFEATISKPDVPLHEAIENIDIGGPSMIRSASKNYRSVIVLVDPSDYEPVMKEIMTS
                     GDVAQDTRYRLMIKAFEHTARYDALISSYFASKVTASSETLPEKINLTFKQIQPLRYG
                     ENPHQKASFYAANFHSKKYYDQLHGKELSYNNILDLDACVRVVSDFEKPSCVIVKHTN
                     PCGAAMHDDLYQAYQNARATDPVSAFGGVIGFNRRLNAATAEALSEVFVEAIIAPEFD
                     SGAIEILSKKKNIRLIRYDFEAARAQGSALPEIRKALDGYLIQDADMRRAGQTEWKVV
                     TRRAPNAEEMHALLFGWTIVKHVKSNAIVYAAADRTLGIGAGQMSRVDASELAVSKSG
                     KSGLSLKNSAIASDAFFPFRDGVDAAAAAGATAVIQPGGSVKDAEVIAAADEHNMAMV
                     FTSIRHFKH"
     gene            complement(26617..26997)
                     /locus_tag="PK937_04900"
     CDS             complement(26617..26997)
                     /locus_tag="PK937_04900"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNH31939.1"
                     /translation="MKYVSALVVLIALAFVQTGYSQMKVDGMKFGTAVEKSEVVGEAT
                     SFPANGSNVYCWIKVVGGEGQTVTMKWYHGETLKNEVPLEIKSWSMRTYAFYEVHRAG
                     TWKVEIVDSTGKVLQSGEFTATAQ"
     gene            complement(27155..27622)
                     /locus_tag="PK937_04905"
     CDS             complement(27155..27622)
                     /locus_tag="PK937_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31940.1"
                     /translation="MRLSKIWLYGIAMLAVVMQFGCGSNKAVMNSKITHVEKKETLGK
                     GEYYQFEITLEDKMEAIEVDGEWRVEDGGDRKARVMVMDEENFLKFKEEEPYKTQYDS
                     GEKIQHTFKIRLLNQFRAQKTLYYLIVDNPSEEDKKEVSFRLDLNYEWGAGSR"
     gene            complement(27699..28997)
                     /locus_tag="PK937_04910"
     CDS             complement(27699..28997)
                     /locus_tag="PK937_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31941.1"
                     /translation="MRKIGLVLKREYISRVTKRSFLITTLVVPFLMLLLPVTIFLIAR
                     NNDAERIAVRDESGLYIQKLQDSRLVQFFYESMPLDSLKRVYRDTKYNYDGVLYIPAA
                     STDPNDGIKYFSSRGLGFTTTLYINSELSREQEKARLSEAGMTKDKIDAIKQRVSFET
                     IIMTEEGESSDNKVVALTTAIIMGIIIYIILIIYGVMVMNGVVEEKANRIVEVIVSSV
                     RPFEMLTGKILGVAAVGLTQLVSWIAIGALLFQGAMLLLGPQMGQFSPDALAQGGIKA
                     RDAEAITVFMKNLDSINFTELFFCFVFYFLGGYLFYAALYAAAASGANDAGDVQSLSF
                     PVTIPVILSFFLLQVSVNDPHGPVAFWASMIPFSSPIVMLGRIPFGVPWWQLALSMSL
                     LIAGFLSAVWVAGRIYRIGILMYGKKITLPELAKWMFYKG"
     gene            complement(28990..29898)
                     /locus_tag="PK937_04915"
     CDS             complement(28990..29898)
                     /locus_tag="PK937_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH31942.1"
                     /translation="MTDVKLSVRDVVKRYQSHTAVDHISLDVQRQSIFGLLGPNGAGK
                     TSLIRMITQITMPDEGAIRFDGQALHESHTRRIGYMPEERGLYKQMKVGDHVMYLAQL
                     RGLSLWDAQKKVQMWFERLHIMDWWNKKVEELSKGMQQKVQFIATVVHEPELLILDEP
                     FSGLDPINADLIKDEIHRLKDEGATIIFSTHRMEQVEEICDRIALVHLGRNILSGEVK
                     AIKQQFKKHHYRIQFDGILPQSGDTAYSIVSQKTGEAVIATQPGVSPNQLLTHLLSRV
                     EMYAFQEVLPSLNEIFIEHVKGYNHA"
     gene            complement(29924..30535)
                     /locus_tag="PK937_04920"
     CDS             complement(29924..30535)
                     /locus_tag="PK937_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31943.1"
                     /translation="MENDELRELWKQGQSLYPQTGMNEKDIRKLLEMRIRTAQLRIRK
                     KLLFELSGMLAILFMIGAVLFVQSHLPLISLAITVASFLFIIPNGFLFILLIRRVSSL
                     DYGIDLRARHTAYLRLHQKTIRIYLRSAYGFSMVLALIVLFIPMEAVNENYMMYFKLG
                     FMGFITVVTFFYRPYVRWMFDKDAQQMTAQLQALQNIETTLHN"
     gene            complement(30522..31049)
                     /locus_tag="PK937_04925"
     CDS             complement(30522..31049)
                     /locus_tag="PK937_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH31944.1"
                     /translation="MSKTKGRMDVISKTFLDLVHTHQGIIYKICRVYAHTPDDQDDLF
                     QEVLLNAWKSYPRFEGASKFSTWLYRVALNTALMYKRKQQRSIVAERVEDGVLENMAP
                     EKSEDTEPLRRLESAITQLEPVEKALIVLYLEEVSYADMAVIMGLTESNVGVKINRIK
                     KQLKQIMEAGNHGKR"
     gene            complement(31189..32043)
                     /locus_tag="PK937_04930"
     CDS             complement(31189..32043)
                     /locus_tag="PK937_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNH31945.1"
                     /translation="MFQPLLLMIYTLAAMVTACEDGPLKPVYPETVTDSTYADRTYIT
                     SVPMLRDGKKYPLVFVFHGAGGNGAGIRSFSGFDKSTWSDSCIFVYPDAKVENWDEGC
                     RCNKPYRLGIDDVGFIDAIYKTMIDKYPVNTRRVYAVGFSQGGLFAHYVACRLSHRFA
                     AVASVASSMNQPLSTYGAPEEKTSVFIIHGKNDGVLPYQGSNEGSFSLLSAPQTLTWW
                     GNYYKMQRTAIGEKAGKNTTLDRYGLESGHEIRLLTPEEEGHQWFFNDFNTTETVLSF
                     FKTKSRTP"
     gene            complement(32046..33353)
                     /locus_tag="PK937_04935"
     CDS             complement(32046..33353)
                     /locus_tag="PK937_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31946.1"
                     /translation="MKYNIVIKIFMHRIPFFVFCWLITMAAYAQTRMEKYQFHYKEAA
                     TAAKDKNYTKMIYHFERADSLRPNNYGILYNLAAGYALTDQADKVWGPLRSALQVNAG
                     LKFYEDEDFDAVKTDSRFVSTRQLAESLQTVIVKSDMAFTLDDRSFHPEGITFDKKNN
                     LWITGSISQRLIGVVRPDGKLDTTFFKPSKPLYSVMGMRIDSLRRRLWVTTSVMPEMS
                     GFTDSLDGRAAVVCFDADRGHEIARFEVSGGHIFGDLCVTASGSVFISDSKAPEIYHI
                     DTDMKKIVKWKTMDGVWNLQGVDVTHDQQYILIADYVSGIYRLNIKTGEVIKLSFVSS
                     SMLRGIDGLLLDGTAIYAIQNGTNPLRILRLSCDPEWSKIKSVEVIDNNADFLGEPTL
                     GCVSGGSLYYIANSPWGAYDKNHQLDITKAAPPMIRRYTPGVK"
     gene            complement(33372..33932)
                     /locus_tag="PK937_04940"
     CDS             complement(33372..33932)
                     /locus_tag="PK937_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31947.1"
                     /translation="MKILVMTFLVFSAHIAGQTKLKLIFMASDSTTVSEKNEYDSIWS
                     SESKKIVRTMERISGMNFIDTLVMVNVVDMPSNSGGTGTPMRLRSSYPTLIKQGTLIH
                     ELGHRLHFCLQRRTKDFNDHELLFLYLYDVWLDLYGEEFAQLQINAENKRSNKTNNYK
                     EMWEKALKLGADGRKIKLKQMVESQK"
     gene            34088..35134
                     /locus_tag="PK937_04945"
     CDS             34088..35134
                     /locus_tag="PK937_04945"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH31948.1"
                     /translation="MINLLYPSWLWRMTIAACIGLFSYPVIMLIRGYTIESTTGYYYA
                     GFSLYAACAHAWMIEWHLFKNRRLRNKLSWLDHTWLRFFIELPLAFLIGATGVFIGLS
                     ALMMSFFDAYNLSLTLWSFYLTLGILMSLVYSAFFHVAELLHAWQSAKVRAAEMEQAS
                     VRAQMAALQNQVSPHFLFNSLNMAHALIDRDTEEAKSFIDQLAEVYRYVLKNRHEELV
                     SVQEEIEFARHYLALAQMRFQYILIKESVSPDALQRAIPPVTLQILIENALHHNAITH
                     EHPLQLYIESTDNSLILRNTRNPRLHAPDHSHAGTGLDNIRQRYLFFTERAIQINQTD
                     AMFSVTIPLLEFPA"
     gene            35131..35883
                     /locus_tag="PK937_04950"
     CDS             35131..35883
                     /locus_tag="PK937_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH31949.1"
                     /translation="MKVLIIEDEPVAADHVASLLRRIMPNVEIAGQIATVREAVLMLR
                     QNRFDLILSDIQLADGSCFEIFRQTKVNTPIIFITAYDQYALRAFEVFSVDYILKPVT
                     EKKMETALNRFKERRDLFFADYEKLTQPSGKEYKQRFLVRIGSKLIPVECKDILFFYA
                     VDKWIDLITKDNKRFVVDFSLNQLEDLLDPKDFFRINRQYIIHRQALISIAQHQPGQV
                     TALIKSNPSESPVISRRVTPRFKLWLSENKKP"
     gene            complement(35922..>36708)
                     /locus_tag="PK937_04955"
     CDS             complement(35922..>36708)
                     /locus_tag="PK937_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31950.1"
                     /translation="KVAEAFARLYGYQNVYDRHQKEFWNSLDEALPDLTPFEAHYMND
                     VFQEAIGNRLEGYVIDVGPRIIRYQSRDKRNSSNYYLDDEDVKFRSTYLGMMLNGQFY
                     HNYSINGQIDFSATGSLDFRSSSKKYSPVSDLKQMQLAVNHLYSIADRLLWTVSGTYD
                     NAHIKIGDEDTHETFKSKKYKISTNLTYFIENRLSISGDAAWLKKKNSPASDLNGYYD
                     MGAMEAIRFVNSGNLFDPYNGVKSSKQWYYGISLTYHFNRTLL"
BASE COUNT        10272 a         8979 c         7656 g         9744 t
ORIGIN      
        1 acgtttcata aaacttctcc tgaattttat catagtggtt cacgttttga acctgcaaca
       61 caatatatac ataattttaa ttaaagtcaa aagagtctta aatctttaat taaaaatttt
      121 atacctcgtt cctacacgca atattatcgg gttaattcat atcaagcttg ttttttgctt
      181 cgcgtgccag tcggtggcgt tcccataatg ggggaaaata ttgtttcttc tctctaaatt
      241 tcttcacttc ttccgcaagt atggcacgaa tagcatgatg tcgctcagaa aaccattgat
      301 ttcgcagagc cgccgtcgca ctcattccct ccgggattgg ccccactttt ccaaaaaaat
      361 tcaaaagcac agaatacgtt ttagccaatc gtgccgctgg taaataccac atgttatact
      421 ctatcggttt gccggcatcg gcatcttcca gcatgatatc gatcacggca ttatccagtt
      481 gatcctgtaa caacgcagat gcatttcgcc attcggctac gacttgcaat tgcggatcgt
      541 attcgagatc cgcgggttcc tgcgtttcaa aatgccgaac gccaaaataa gacaaccatt
      601 gcttagtacc cggcgaagtg atgcgcgaaa tacccatcca caaacttatt cctaccgatt
      661 catacgctga cgaagccact tcggaacaaa acaatgcacc atgatcccga tagttcataa
      721 caaaatcgta cggaatgtgc ttttgcaagg ccaactgaag ggcaaaatcc gccgctttat
      781 gcggtgccat cggatcgctc gctaaaacag gaatatcatg gcgtgggcgc agtatcaaaa
      841 tgcgtaattt tttatcggcg atatattcat tccatgtagc gatcgcaaca cctttttcaa
      901 tgtgcgcctc gataagggaa atttttccgg tcaccgaatc aatatgaact aacgccacat
      961 gagaaaaatt accgggaaaa tcattacctc gggcaatcaa tgccgatgtc ggcgcaccgc
     1021 cgcgcgacac caaaatatca ccgctatgta acatgacacc taaaacatct gcatgtggtg
     1081 taaccgaaga ctcagcatgt ccaggattaa tctcggcaaa tttatcggtc tgcgattgta
     1141 atagaacttc ttcaagtgcc gtacgcattc cgtacaaaat ccgataaagt ctttctctgg
     1201 ttatacgatc tttcatatcc cagttacggc tttgtttctt aaccacaaat cgtaaattca
     1261 taatgatacg cgaaaaatca gcaacgaatt ccggctttac cgcaacacga gtagctaaat
     1321 aataaacact tctttcaata cgccaaagta actcgtcact tgtgttcact gtagtcgtat
     1381 tcagcgtgtc cgtcattctt tgcagttgag ccatggatac ttgtatcaat ttgacagttt
     1441 gtaaaccctc ttcataacgt gatgccacaa attcacgctc cagcgtttgc cacaagctat
     1501 cctgattcca ttcaaatgag tgatcaaacg aagtagtatc aattttcaat tcgctttcag
     1561 gaattaataa tatcactaca cataagaatg ttcctaagaa gagcagtttt ttttgtgatc
     1621 ttttcattgt acctatttta tattgttatt cctattctgt ataattaaaa tacgaatatc
     1681 cgcgaaggat accaattctc tatacgattt atgaaatata attttggacg tgtatcgtgt
     1741 ttgataaaat catatcattt ttttctattt ttcttttgat tgtcctcgct aactcgtgcg
     1801 agtcattatc ctccaaaaat acttcattaa gttacacgcg attggggttc aagggcaatg
     1861 atttattaaa gtctgttcat caaaattcgg acggttcctt tattataata gggcgtaccg
     1921 ataactatag aggcaattca gacatattga tagttcttac tgacgcctac ctcaatccgg
     1981 tttggtataa agccattgga tccgaaaaag cggactacgg cacggatatc acagaaacat
     2041 cagacggtaa ctttctcgct gtaggaacaa tccaaataac cggtaataat aaagacgttg
     2101 taacactgct cttagaccga agcggtaaat tacttgcatc caaaagctat gggggcctag
     2161 cgccggatga agcacaacgc ctcattaaat tagattatgg cgtatttgct atgatcggga
     2221 ataccgggag tttcggccat ggtgcaagtg atgtctggtt gattttatta gatgcttttg
     2281 gcgatagtac ccgatcatat acctttggta cagaggatga tgaacatgtg ctgggtgcca
     2341 tacaatccca aaacggaagt atctactgta ccggttatgt aagcgctgca gaatacgtac
     2401 ctttcttact tacaatcaat tcagatatgt caaaccatct ttgggtgcga tatcctgcta
     2461 gaaattctcg ggatgaggca tacgatatta aagagacatc caatggcgat ctcattatgg
     2521 tcggtcgaac caatatattc cgatcaccat atgaaaatta tgatgcgcag gtttttaaga
     2581 cagatgcatc cggagccaaa tcatgggtta cgcgatttgg caaaaccaaa tacgacatac
     2641 tcgaatcttg tattccgtta gatgacaacg gttgtattgt cattggcagc agcctgcatg
     2701 tcagtcccaa gtatgtggcc aatcaacaag cgctaatagc acgaattcaa tccaccggtg
     2761 ataccgtatg gactcgtcaa atcggcggtt ctccaccgga tgaagccgtt tgtatcttgc
     2821 cgatcggatt aaatcgtttt ttgatcggcg gtaatactcg taatggccag tacggcgatt
     2881 gggatattgt tttttttatg atagacgaaa atggcaatca tgacttcaat tgaaatacat
     2941 cattacttcg cttccatatc acgcgctcat aatttgacct aaatcaccat cctaacacat
     3001 cccttaacgc ctgcactcgt ccccgcgcta cgtcaatttc cgaattcttt ttatcattga
     3061 gaatgatttt ataggcgccg ttgaaccacg gcgtcaactc gcggatcgca ttcagattga
     3121 cgatcgtagc acgatgcaca cgcaaaaatt cggacggcct gagacgttgt tcgatttcat
     3181 ctaatgtgta gcgaatatca tgcgccgatt ggtccgtatg caggtacgtc acgctgtctt
     3241 gggtgtcaaa ccacaaacat tgcatggctt tgataaagcg aattttttca ccgtcacgat
     3301 acggaatgcg ttccagatac gcagtttgcg tgtttttttc ctgcatcaca tcggttatca
     3361 gtttttgtat gctaagtgtg tttgcggatt cgggaactgt gagatgcgcg atcacgcgtt
     3421 cgatagcacg ttttagccgc tcttcatcaa acggtttaag cagataatca acggcattta
     3481 catcaaacgc acggatcgca taatgatcgt aggccgtcgc aaatatcacc ggcggaactt
     3541 ttttgagatg catcaaaact tcaaaaccgc ttaatcccgg catttgaata tcaagaataa
     3601 tgaaatcggg ttgcaaactt tcgatttttt ccaacgcttc gatgccgtcg gatgcttcgt
     3661 ccatgatcgc gatttgcccg gcaaacgata ccgacagcat atccctcaca cgttggcgag
     3721 cgagcgactc atcgtctata agcatacaac ggaacataaa ttaactcact cgtatcggaa
     3781 ttcgcacttc ggctacggcg ccgccctgag gtcggttgga caaaatcaat tgacctttgc
     3841 cgccgtacat tttttcgatt cgttcgcgta cattttttac accgacgcct tcgcgattaa
     3901 aaatcacttt accgttgcga tccacagaaa aaccttcgcc gtcatccgaa atacgtaaaa
     3961 ttaaaaaatc gtcttgacgc gacgccgaaa tttccaatag cccattctct cgccgcgggg
     4021 caaggccgtg tttgatcgca ttctctacga gaggttgcaa aataagtccc ggcactcgta
     4081 ccggacgtac cgttacatcc acattttcac aaatggtaag tttttcgccg aaccgaacgc
     4141 gttcgatgcg caaataatta tccaaaaaag taagctcttc gtccaacgtg acggtttcct
     4201 tttccgaagc gaccagcaca taacgaaaca cctccgataa acgctggacc atcatttctg
     4261 cgcgtttggg atccgatgcg atcaaagcgg ctatggcatt cagcgtatta aaaagaaaat
     4321 gaggattgac ctgagcccgc aatgcacgca actccgcttc gaccgcggct tcacgtaatt
     4381 cttgtgcaat tttcatttta ttcagcattt cacgataaaa ggctactgaa taaaagctga
     4441 ccgtaatcaa taaggttaaa atgacagcga taaaaacttg cacatagatc atgcgcagcg
     4501 aaatcaaaaa attaataccc gtgataattt cagcgagcat aaacgccaaa aaacttccca
     4561 ccgttcccag tattactcgg ataagtatct caatagcaaa aaactttttg ggacgctcca
     4621 aagtacgcgg aacaaaccga cgtatcaccc gttccgcaaa cgtgcccatt aaaaatcgca
     4681 tgacaacata aaacgaaaat gatacgcgca gggaaatcca ataattattc catgtccaca
     4741 ccggcacata gagcgcgccg atgataaaac ccgtcgtaca gatgactacg aaagcccatg
     4801 cgatctcgtg aataataaaa cgttttatcg tgtcgttgaa tttcatgacg ggcactcgtt
     4861 tctattccca cttaaataat ttgacagcta ccgcataaac gatcacaccc caaacgccca
     4921 ataccatcaa ttcaaaacct acgtcggcaa aacctgcgcc ttcgaaggct attttgcgca
     4981 gcgcatcatt gacatacgtc aacggtaaag catggcacac cggctgtagc cattcaggga
     5041 aaacacgaat actaaagaac gtacccgcca acaagaactg cggcattacg atcagattgg
     5101 cgatgggggg gacggcttgt tcatggcgcg ctatatttga aacgatcatt ccaaaaccca
     5161 aaaacaccat gagcgcgatg aggcaaacga gcaacatcat cagcagtgtc acccatccgt
     5221 tgatgagcgt gaatccgaaa acccatttgc cgatgagtac gatgatggat acttgcgcca
     5281 acgcaaaaag catccgtgca atggcctcgc cgaccaaaat cacgctcttc ggcgccggtg
     5341 tagcaaacat tcgtttgatc accaatcggg acttgagcgc aatgagtaaa tacgccgcgc
     5401 cgaaaatacc cgaactaagc accacaaatc ctaactgacc cggtaaaata aaatcaatcg
     5461 ttttgtatcg tcgcccttct atggtcgtgt gcgcgatcgc aagcggcaca tgctgcactt
     5521 gcatcataag tagattggcc tgtgcggtaa gaccgcgcat catattaagc accacgatac
     5581 cattggggga agcgtcggag gtctgaatat gcgcaacata accggaatcg gttttttcaa
     5641 cgcgcatcag agcatctact tcacctttgc gtagtttttc tgtcagcgct acgtcatccg
     5701 gattgcgcat caattttatt tgatccatgt gactgagcat ttccatcatc gggttttctt
     5761 gcgaatcatt ttgtatcacg ccgatggtca aattcaacgt acgatttcct ataaaaccga
     5821 aaatgacgat gaaaatgacg ggaaaaataa atccgaagac aatactcgac ggattgcggc
     5881 tgatcgcacg aaggctcgta ataacataca cacgcaacgc gtgaaaatag gaataaggtt
     5941 cgggttgatt catcatggtg tcggtttttt caaaatttaa gcgtcgcgca gttctttgcc
     6001 ggtcaactgt ataaatacat cttcgagtgt tgcttcgcgt acacgggtcg gccgttggaa
     6061 ccccgtagca agtaagccgt gaataagtcg ctccggtgaa tccagcgcta tgatacgtcc
     6121 ttcatccatg ataccgacgc gatcgcataa caactcggct tcgtcaagat agtgtgtcgt
     6181 catgataacc gtcgtaccgt cgtcgcgaat ggatcgaatc aattcccaca aattacgccg
     6241 ggcttgcgga tccaatccta cggtgggctc atccaaaaaa accacacgcg gtttatgtat
     6301 taacgtcgtt gctatggaga agcgctgttt ttgtccgccg gaaagtttat ccacacgcgt
     6361 tttggcctta tccgtcagac cgactttcgc cagcaaagcg gatgcatcat gatgcacatt
     6421 gtacataccg gcaaacagtg cgcacacttc gagtaatgtg gaattgggat aatatcccgc
     6481 ttcctgtagc tgcacgccga ttcggcgttt gatcggcatc ggatcgcgat caacggaaaa
     6541 ttcttcgatc agaacttcgc cggaagtttt ttcgcgcaat gtctccatga tctcaagcgt
     6601 tgtcgtttta ccggcgccgt taggaccgag caatccgaaa atttcgcctt cgtacacatc
     6661 aaaactaata ccgcgaacgg cttcaaagga gccgtaattt tttttcaggt cacgcacctg
     6721 aacgattttt tttctctctg acatatattt ctcgattatc cgaagttaaa gtcaatatac
     6781 ctttttacgg caatttttaa accgatttat tttacactaa aaaacggtca taaactcaaa
     6841 ctaaaaccca tcacaacggt tcggttgcta aaataactcg gcacactatg acgttcggaa
     6901 aaatcctcgg aatatgaata atcaagaata tttcggatac ccaaaagatt gagcgcttca
     6961 acatataaca cgacatgttt ctgcgggccg aggataaaaa acttcgacag acgagcatca
     7021 agccgcttga aatccgggaa ccgatcggaa taatcggcgg cgtaaaccgg ttgccaaatc
     7081 ccgcgcaccg gatcgtattg accggcggta accggcgtat agggtcgtcc tgtcgcatac
     7141 cggagtgtcg aagccaattc aaaattgcgc ccaaggtttt gtttgacgat caatgaaata
     7201 ttatgggtga tatcgtaaag actgggtcgc atagtttgaa tctgcatttc ttttcgccgc
     7261 gtctgaataa aactatagga aaaccaaccg ctggttttcg caaaaccgcc cttcagaaaa
     7321 aaatctaaac cgcgcgcata cccgcgccct tgactcacat agtttttatc ttcatcggaa
     7381 agcggcagat gatcatacca tttgtagtac gactctattt ttaactgata cgtcgcagcg
     7441 tttttttccc acccgacgac ggcgtggcgc gcacgcatcg ccttaaggtc aggattaccg
     7501 atggttttat catacatcgt aatgtgcgga aattggtgaa atgtcccggc tgaaaaacgt
     7561 aaaaaagaat tcgcggcgaa ttcatgcaca acggatccgc gtatatcata tacttgattt
     7621 tgcgacaagg tttgataatc ggtacgaaaa cctatttgtg ccgaccaacg ccgcgttatt
     7681 ttttgttgca cttcggcata cgccgctaca acggtggcat ggtgatttcc ttgcaaagcc
     7741 acgctatcgg cgccattgta atattcgccg cgccgcagcg ggagtatata atcaaaccgg
     7801 tagaaatcac cgtaggcttc tccgccaaaa ctcaaaatgg tccggttaag caatgcataa
     7861 tgatgatccc agcgccattt gatattgtac tcacggttcg cccgccgcca gtcaccgaac
     7921 gtccagtcgt tacggtacat gctgtacgac cacgacgcct tacttgtcca ttgggggtta
     7981 aataattccg accactggag actcatgacg cgattgccag tttccaaggc gtaccgctga
     8041 ctattggact gcaacgtcag gtcaaacgtc tgtgcatcgc gactttccaa tatcatagcc
     8101 tggatttgcc ccgacggacg gtaattataa ttaacgatgc ccgtgacatt gctcgttacc
     8161 ggatcacgca caacatcaaa agccggtttg ttcattttaa aaagcaaagc cgtcgaggta
     8221 tgatccgcat agacctgcgc ggaaagcgta ttgggaataa tctgccgttg taatcctccg
     8281 ccgaccgata cgagattggc atccagtgct atcctgtttt gagccacgtg attatccgtt
     8341 tcgatatcca ggacgccgga taacgcattg ccataccgca cggaaaaacc gccgctggaa
     8401 aaatataatt ttctgatcac ggcggttttt acaaacgtaa aannnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccgccgtatt ttcgccatga
     8521 tacggatgag ccaacgtcgc accgttgaac agaatcaggt tttcggaagg actgccgccg
     8581 cgtacgggaa cagccgctgt ttcatccgtt tgtgttacac ccggcaaaac cttgacgctc
     8641 tgaaaaacat cggcggcgcc gcccggcgtg gtatatacat ctattttaga aacggcgacc
     8701 ttgccttcac cgccgccgta ggcgctggcc tcgatcacca gttcgcccaa ttctaccgac
     8761 ttctcatgca tggtgacatg cattgtttga gcatgaggaa cttttatttt cttaacttcc
     8821 gtatcatagc cgatcaaact cgctgtcaga tgacgctctc cggctatgtg ggtgcgaata
     8881 atcgccaaac cgctgtcatt ggtaaacgct ccggcaaaat cgccgtcggc aaaatacaca
     8941 ttggcgtacg ccactttttt tccatcacta tccaccaact gtacacgtac tgtatatggc
     9001 gcggccgtct gtgcgtaact ttggtattgc gcgcatagca gcagaactac gataaaagtt
     9061 tttttcattt ttataccgga ttgattattg cgtgtttttg ttctcaagcc gcatcaattg
     9121 cgctttagcc cggcgcgatt cgggattgat ggtcaatgat ttctgataac aacttttagc
     9181 ttcgttaaac attttttgtg cttcgtaggc ggatcctaaa aaagaataac actcatcata
     9241 gccccatgtc ggcgccaacg gatccgccgg cgggttggcc gcttcttctt catacaaacg
     9301 tatagctttg aggaaatcgc ccgtcgcttt atctgcaccg ccgccaaaca tggacggcgt
     9361 attcatcgta ccgataccga taagataaaa aacacgagga cttttgggat ccgcttcgac
     9421 ggctttactc atatactcct gtgatttagg gccgagccac atagccgata atggattaaa
     9481 acgaattttc atacctgtga gagagcccgt caaagctaac gactcggcat gacccggttt
     9541 gagcgtattg gctttttcca atagcgacaa ccctgtttcg atgtattgtt taccggcgtc
     9601 ttcatccggt tcaccaccgt atagtttcat aatcgccaga cgatagtatg caaatgcgct
     9661 ccaatacaac ggccacacat tctgcggatc ggccatagcc gcccgttcac acagtgcgct
     9721 gccctcgcgg atcatcgttt cgttccatgc ggcaaaccca ttttgaattt tgttttttcc
     9781 ttcgatggct atagataccg ccggattttg tgaaaaaagt gttcctgcgg tcaacatcat
     9841 tacaacgaac atgcgcttca tacatcctcc tctattgtga taattaaaaa ttaacgtaca
     9901 ccaaatccag cggcgccaaa aagcaatgtc gcataaggcc cgttccacaa aggaatactt
     9961 cgcccttccg tatcgccgcc aaagcgttgc caatcatttt tagccggtgt gtaacggtat
    10021 ccgatttgca taccgatagc caaaccgcct ccggaacgtc gcccgctgtc aaacagcgta
    10081 tgcaatacgc cccatccggc atcaatggtc gttgccttga cacggagtct tgaagctctg
    10141 gccggttgat ccaatatatc ctcaaaatcc gccggaatac gctcggcgat ctctaatata
    10201 aaatcagagt cacccaatcc gacaaacgga taaaagcgta ttgatccttt ttgtaaaagt
    10261 atgtatccga ttttaaactg ccccacagcg ccgcgcagtt ttacccgata cagcgcattc
    10321 tccgataccg tgttgcgggc catcaaagca tggctttcac cgccaaacaa gaaccgcccc
    10381 agcacggcaa aacctccgcc gccatacatc cagctttgat tttcgtaagt gcggtatccg
    10441 ctattcttga gtgttttatt aaaaccggtc atatcttgtt tactcgcacc gaaaataaaa
    10501 ccgccgaatc cgccataggt tttacgaact tgctgcgctg agccagggct gacggtaatt
    10561 aggattatac acacgagaag ccatacacga taccgcataa ctttccttcc atttttaata
    10621 acaatacgcc caataccgtt tttttccgac atacttccgc caaactgcag taaatggggg
    10681 ttgaagtgca caatactgca tacgaaaaga ccctttgtgc cgatgcatgc aaggcataca
    10741 accctatttt caaaaagtgc taacgtaggc attgaaaaaa aatttgcttt ccgatgttag
    10801 attcgttaat atgaagtccc cacaagaggt ctccccgacc tctggctacg atagagaccc
    10861 ggaagcttgt cttccgggta tttttttgta atgatgttct ttttatgccg tatgacagac
    10921 aaaattacat ttgactttca tgcgccgttt atataatatc gaatactatt ttgcacactt
    10981 gatgtcctat ctcaatattt tctaggagga atttccatgg ctctggatgc attaggcatg
    11041 gtagaaacca aaggtttggt cggcgcaatc gaagccgctg acgcgatggt caaagccgca
    11101 aaagtcgaat taatcggcaa agaaaaaatc ggcggtggtt acgtgaccgt catggtacgt
    11161 ggcgatgtcg gcgctgtaaa agcagctacc gatgccggcg ctgcagcagc agaaaaagtc
    11221 ggcgaactgg tttccgtcca cgtcattccg cgtccgcatc cggaagtgga acttattctt
    11281 cccaaaaaag gctccaacta atttaacacg gcgaatcgta tggcagatga acgtgcatta
    11341 ggaatggtgg aaacgctcgg attggtcggt gcgatcgaag ccgcggatgc catgatcaaa
    11401 gcttccaatg tcaaactgat cggcaaagaa ataaccgatg gcgccatgat cacgatcaaa
    11461 gtggtcggcg aagtaggcgc tgtgcaggcc tctgtgtccg ccggcgaagc cgctgcacgt
    11521 cgtgtaggac aagtcgtttc cgttcatatc ataccgcgtc cggatgcaat gacggaatcc
    11581 atcatctatg atgaaggatt ggtagaagct ccgagcggcg gcacgggctc aaaaaaaaaa
    11641 ctgaacgcct gacccttgcc caacaagatc tttttaaggg tgttcaaaaa ggcgaatcca
    11701 tacttaagaa gaaaaagtta gttgttcctg aaaccgatta tcatgagttc agcgtttcgg
    11761 aacttcggca ttatgcacgg tctgttccca atttcccgct aaaaggccgc gaaatttcca
    11821 aagccaaccg ggatatgctg atcgaagaac tggataaaat aattcctaaa aaataaacac
    11881 cctgaacatc cttcagggtg ttctttttta cgcttcattt ctacgacgta tgtttaaatt
    11941 gcccgaatgg cttctgcgtg ccgaccggcg aattcaggag cttaatctct ttcgccggat
    12001 cttcatcatg atgtttccgc tcatgatcgc ggcgattggt gtatttgaaa tcaccatacg
    12061 ctggtttgat atccacgcta tcgagtacgc acacatatgg atcatactaa ttccgcttat
    12121 tttttctatc atcatatcgt atttatcggc tcgatttacc gcgaaagaag ttgcccatcc
    12181 tatcaaactc ttcatcgaaa gcgccaaagc catcgcgcgt ggtaattttg atcacaaggt
    12241 caatgtgcat accagcgaag aaatgactca gctttcgcgc atttttaact atatgacccg
    12301 tgaattgaaa gagatcaatc agggcaacat ccgaaaaaca atccaggaaa aaattaagac
    12361 cgaagccatc attcgcaata ttgccgacgg tattatcgtg gtcggcccgc tgaatgaagt
    12421 tctgatgatc aatgaagtgg tcgaaggatg gtttcatgta caacaaaaag ccgtcatcgg
    12481 tttttcgctc aattttttct ttcccgaact caaatttctc gtgaaacagg cgcttgacac
    12541 acaaggcgaa acggtttttc aggacgaact tacgatcaag ccgctcaata gcccttcgga
    12601 aatctatctc ggtgccaacg cttccaaagt catggataac gacgatctga ttgctttggt
    12661 tattgttctt agaaatatta cgaaagaaaa agaaattgat cggatgaaaa ccgaactggt
    12721 gaatgtcgtc gcccatgaac tgcgctcacc gttgaccagt atcgccggtt tcagtgaaat
    12781 catcaaagat ccgcatttat cggaagccat tcgtaaagaa tacattgata ttatccacta
    12841 cgaatccggt cgtttagctg aaatgatttc caagtttctg gacatcagtc gcattgaaag
    12901 cggcaaaacg gttatcaata aagtcccatg cgatcttacc acggttgtag ataacaccat
    12961 caccgccaac acaccgatgg ctcaaaagcg cagcatgcac gtcagcagca cattgcccaa
    13021 atgggttcct ctggttcacg ccgatcctga tctgatcggt caggtcgtac tcaatttctt
    13081 ttccaatgcc gtgaaataca gccccgaagg ttcagaaatc aagatgcgcg tcgcggtcgc
    13141 ggatgatacg gtgatcgtgg aagtgtccga taacggctac ggtatatcca aagagaatat
    13201 gaaacatctg tttcagaaat tttttcgcgc taaagatgag aaacatgtga aagatgtgga
    13261 aggcaccggt ttgggattgg cgttcgtcaa agaaatcatc catcagcaca atggtaccat
    13321 acatgtcgag agcgaagtcg gaaaaggttc ttctttttcg tttgccctgc cgattgattt
    13381 tacactgccg gaaaatgctg cgctcaaacc cgcctcatga tatctttttt aagcaatgaa
    13441 ctcctgatct ttgtattgca actgatacaa cctgtaatat aatccaccca tagccaataa
    13501 ttcttcatgc gttccgcgct cacggattcg ccccttgtgt aaaaccagaa tctgatccac
    13561 gtgtctgatg gttgacagac ggtgggcaat aataaccgac gtgcgtcctt gcatcagttg
    13621 ggctatggct ttttgtatca ggcgttccgt ttcggtatcg atacttgatg ttgcttcatc
    13681 caaaatcaat atccgcggat caaaaaccaa cgcgcgcgcc atcgcaatca attggcgctg
    13741 gcctaccgaa agcgtgctgc cgcgctcttg cacgggttca agatattgta aaggaagccg
    13801 gcgaataaaa tcatccgcac cgactcgtcg cgcggcttct tcgacctgcg cttcactaat
    13861 atcatgacga cctagacgta tattgtctaa aatcgtaccc gaaaataaaa atacatcctg
    13921 taacaccacg gccatcgtgc gacgcaaagc atattgatcc atccggcgaa tatccgtctg
    13981 atccagtaaa atatcgccgc gctgagcttc ataaaaccgc gacgcaaggt tgataatcgt
    14041 acttttgccc gaacccgtcg caccgaccaa tgcaacactt tgaccgggct gagtaacaaa
    14101 cgaaatatct tttaaaatat agtcttcatc cgagcgtata tcctgaggat tataagcaaa
    14161 atacacatta cgaaattcaa tgcgtcctga catcactttc ggcgttatcg gttgagccgg
    14221 attaggtatt tccgtcggtg tgtctatgag tttgaaaaca cgctccgacg aagccatcgc
    14281 tgtctgcaga atattgtatt tatcactcag atcctgaatc ggcctaaaaa acaacatgcc
    14341 gtactgtata aaagcaatca atgtacccag tgttattgac gaagagcgca cttctccgct
    14401 tccataccaa atcaacatgc cgatggcaaa actactcgcc acgttgatcg ccggataaaa
    14461 ccaggcgtaa tgaaataccg aatccaaatg tgaatccgtg agcgtacgat tgagcgcatc
    14521 aaacttttca aaatttttct tctcacgatt aaaaatttga accgtggtga cgccggtgat
    14581 attttcctga agatacgaat ttaaagctgc gattttaaga cgcacttcgc gaaacgcgac
    14641 ccgtaccttg gacttgaaca caacggatat atagaatatg agtggcaaaa tggccagcgt
    14701 aacgagtgtc aaatgtacat cgagaacaaa catcatgaca ataatgccgg ccaataaaaa
    14761 aatatcaccg aaaagataga caagcccgga agtaaacata tcgttaagcg attcgatatc
    14821 cgatgttacg cgtgtgataa gtcggcccac ggggtttcga tcaaaaaatt gcaaatgaag
    14881 atgctcgaga tgcgtaaata atttcatccg cagatcaaaa atgatacgct gacccatcag
    14941 ttgagtcaca tagatcacac agtattgtaa aagaaattcg ccgatcaaaa gaacgacaaa
    15001 caagatggca aaattgcgta aacgtaatcc gtcaccggcc agtatagccg cgtccacggc
    15061 ttcctgaaca agtttgggac ggtatgcgct aaaaccctga gcggctatta ataaaatcac
    15121 agaaacaacg gccatccatc gatacgggcg aaccaaactc aatagcctac gcatcaaacg
    15181 cgcatcgtag gctttcccca tgatatcatc atcttgtgtc tgcatcgtgt attattgtct
    15241 ttcgtaatga tgtaataatt tttggatcgg cggacgtatt agagcctcgt ttcctataat
    15301 tgctaaaacc caatgtttgg gatcaatcat atcagcggct tttctcagat catcctgctc
    15361 taacgattgt aactcggcca attcgcgttc aacgtaatcc ggcggtaaat caaaaaaagc
    15421 gccttgctcg atcaataaag ccatgcgctc aatggtttcc atcgtaaacg gatgatgtcc
    15481 tgaaaaataa agtttggcat cctcgagttc ctgatccgac acgccatcgg ctttgatggc
    15541 ttccagttcc tttacaatag ccgtaacggc gcgaggcgcg tgagccgcgg aagtctggag
    15601 tacgacagaa aacataccac cgaatttacg ttttgtaaga ctggaatata cggaatatgc
    15661 caaaccttgt tgcgtccgga tatgatgggt taatcgggac gataaaccgg cgccgcctag
    15721 aatataattt agtaccatct gcggcacatg gcccggctca ccgcgtgcgg ggccggtatg
    15781 gcctagcgca atatgcgcct gtgcgatcgg acggtcggtc aaacaaaaaa tacgttgtcc
    15841 taatggttgg gggctatcca tatgaatcgc gtttatatct attgaatttt taaaatggga
    15901 agcccagtta ttttcaacaa acgttctcat tttattttca tccacatcac cgaccaccgt
    15961 cacgatcgca ccgcacgcgg tgagcttttc gttataaaat cgcagcgctc catcgcgcga
    16021 taaatcatcc acaccgcttt ccatgccatc ggtcgagcgg ccatagggat gtctaccaaa
    16081 taacgtactt tggaataatt cacccaataa aaccccgggc atatctaatt tctttttaca
    16141 atcggctttc atttgccctc gtgcacgttg caactcgtgt tccgggaaaa cagcatccgt
    16201 aagaatttca tgcacgatca aaagcgccgg ctccaattgc tcacggctta cggttagatg
    16261 taatgtaaac gcatcatggg taactgatac ggagagcgaa gccgccagat attctagtgc
    16321 atcggctata ctcagagcgg tttgtttgga tgtgccggat tttagcgtca gcgcaagtaa
    16381 cttacacaga ccgggttggt cggccggttc atgcgcgata ccgacaggaa atcgcaagcg
    16441 caaaaccaca aaaggcgtgt gtgtggattg acgaaggtga agcgtccacc gattggatag
    16501 agtgtgggac acggatccga cgaaattaga aagcggattg aattcggaca cttctaatcg
    16561 cgcggattgg taaatttgag cgatttataa tcttgtccga cgatgacggt aacctcgagc
    16621 atcaactgtg ggtttttctg ctgaataatg cccgactcgc cgacaccaag cgcgacggcg
    16681 acttgttgtg caccggctaa atttccagcc cgatcctgta cgattgtttt tttgtaattg
    16741 gaattcggcg cattgcccgt actcacaaca tcaaaccctt tactgcgtag atagtcggcc
    16801 gctttggcgg ccagtttggg tacgccgctg ccgttgagca cttcgatacg gattttctga
    16861 ggacgtgatt gggtaccatc aaacgtggtt gacggtgaag tctccgcagg cacttcattg
    16921 gtctgtgttt cggcttctga tgtgccgctg ctattgatac gaataaccaa cgaaatcacg
    16981 agaattaaaa cgacaaggcc caaaccgcca atcatgtaat tcaaaatttt gtcccacttg
    17041 ggatcgtgaa taccggtcat cgtcagttgg gtcggctccg gcttcggatc ttgagttcgg
    17101 gttgccgatg tttgatccaa agtctgtgtt gattctttcg gacgatcgtc cgcgcgcact
    17161 ttgcgttgca tatccactcc gtaaacaatt aatcccgacg gtatcgggat taaaggtatt
    17221 ttttaaggcc tcataaatcc gtaatcgtca aacaatcctc ccgaaccgct tcgttgtgga
    17281 cccggattaa aaagactatt accggtataa taaggattgt tactgtatcc cacttgcatc
    17341 tgaaaatgtc ggctcggtct ataaaccaat tctgctccgt gcaatatttc acggtttttt
    17401 tgcattagac tattcttatt ctgaccgaac gactgatgca tcaatcccgt attgagacgc
    17461 agcaccaaag gatccgagag acgaatatta agcgaattgg aatacatacc cagagtaccg
    17521 ctcgcgccgc caccgctata atacgtcatg gcataactct gcgtcatcgt cacacggtcg
    17581 gaaccgataa aactgagaag cgatccggga acggctgaag taatcccgcc atgcacatca
    17641 atgggttcac gcccagtctg tgttttgagt tgcgcggaaa gcgaaactga acaaagcccg
    17701 aaaatcaaaa ggaatatcgt ttgttttttc atgctaaccc tttctacgct tagctgaatc
    17761 aatatcaaaa aaaggtataa aaaagtcaag agaacgcagc tcggttttta tggtacctcg
    17821 atcacgtacc cgatgccgta aaccattccg tcagcgataa atttttcata tacccccgtt
    17881 tgaccgatag ccgattgcac ttcccgcgca attacacctt cgatcacaac actattttcg
    17941 gtgacattgg gttcgtaata actaacgtca cgcgtataga cggaggacgg taaaaccgca
    18001 cgcacaggcc acacaggcca ttgcgcaccg cgcgctgaac ccgatttttt cacatagagt
    18061 cggatacgtg atgccacata aggacggagc gcctcatatt cccatgtatc catttcttga
    18121 ttataataat gcatactatc caaccaaccg ccttgaactg aaaatccaag ccctcgcaca
    18181 gcgagcgtat ccgaacctgt gatccactct atcactggaa tcgccgcaga cgtatccgca
    18241 gttttgcgat gcatagcctt cacagaatcc ctgatgtttt gtatgtgttt tatacgttca
    18301 cgaaacactt gcggtttcat atgaccgagc atccagcggc gtttaccttc gttatatgcg
    18361 gggcgtatta caacaccgtt ggcataaatt gtcaaagatg gcgcattgag ttcctcgccg
    18421 gtccaagagg ccgcaccgcc gcgagcccgt ttggaaaaaa caatcggact ctccggttca
    18481 atcaaaacat gccgcgatgc gcatcccaca acactaaaca aaaaaataag actgcctaca
    18541 aatttaacca tgaaacggct ccaccgcgat atcaagcaat tgtctgaaat cggtcaattc
    18601 gtaatccgca ccggattcgc gtgtatcaaa tgtatcgccg tatcgggcaa atgccgtttt
    18661 cattccgatc tgtttggcac cggccatatc gcgttccgcc caatcaccga ccatcaaagc
    18721 gtgctccggc gggatatcca tctgcgttag cgctttgcga aacggctccg gattaggttt
    18781 acgctgaaac gtatcttcgt acgtcacgac ggcatcaaag gtatgatgaa ggcgcagata
    18841 acaaaggcgc agccacgctt cacgcgtcgg cgcatcactg acaacggcca atttaaagcg
    18901 acgtttgact agttcattga gcgtagataa aacatgcgga taggtgacca gcgaagcttc
    18961 gcgggtctgg cgatacgcca caataccgtt ggctaaaatt ttgtggtcaa tgcggccgag
    19021 cgcattctcc aaaaaaatat caaacacctt ttggtattcg tagccttgcg aattatagat
    19081 cgtccatatt ttggagataa tatcttcttt ttttacctcc aagccggcat cgatcatggc
    19141 ttccgccgca ctggcaatcg cacgttcttt gaggcgcata aaatcaacaa gggtattgtc
    19201 gagatcaaaa ataatcgccc tcagatggat atcgggtttc accatacaaa atccatttag
    19261 tgtatttcgt aatttttttt gcgcatggcc acatttaaaa caaatccgat caggaacata
    19321 ttggcccaaa gcgccgagcc gccataactc aataaaggta agggtatacc cgtaaccggc
    19381 ataagacctg tggtcatgcc gatattgaca agcaactgaa acgcaaacag cgacgcaatt
    19441 ccgattaatg cgaggctggc aaatttatca tcaaccgcat cggcaataat gacaattcga
    19501 aaaataagga gaccgaataa cgccaacaaa aatagacttc ctaaaaatcc gaactcttca
    19561 ccgatgaccg aaaaaataaa atccgtatgt tgttccggca aaaaattgag ctgtgtttgt
    19621 gtgccgtgaa gatacccttt accgaccaag ccgcctgaac cgatagcaat cttggattgt
    19681 gtaacctgat aaccactgcc ttgcatgtca cgttccggtt ctatgaaatt gaggatacgc
    19741 tgcttttgat aatctttgag cgattcccaa atcggttccg atgaaagtcc cacaatcaca
    19801 ttgacggcaa aaacgccaag gatgatcagt gtttttcgtt ttgaaaaata cagtatggtc
    19861 agaataataa acagcatcgt aataaacgca ttaaaactgt aaccgctgat gatgtgcacc
    19921 agtacggtcg caatcggcga aaacataacg aagatggaaa agagcgaaat accgttccaa
    19981 tacaggatag gcaatgctac gctgcccacg caaagcgccg tacccagatc gggctgcttc
    20041 ataactaaca gcatcggtat cgccgcaacg gaaaacacga taataatatt catcacttgc
    20101 gaactgttga cttgccgcaa tgccaatatc cgggctataa aaagaatggc tgcaattttg
    20161 gcaagctccg acggttggaa tccgaatggg ccaagagtaa accagcgccg agcgccataa
    20221 cgtaccgaac cggcaaaaaa aactaaaata agaagcgcta tggtgactgc gaaaacagta
    20281 aacgaggcgc catctatcaa tcgcaacggg ataagcaccg tcatgagcat cagtacgaca
    20341 cctataaaaa agaacgtgag ttgtttggta aagtttaatt gatcactcgt aaacgtcgca
    20401 ctgtaaatac acgccaatcc taaaccgcac aacatcaatg cgggcgcaag aatcagataa
    20461 tccaagtcct gtaaaaattt tttaattcgt tgtaccatga acttcgacta cggccgcttg
    20521 cggggctgta gtatttttag gcgtttcttt gcgatctttt tctttcggat caaacgattt
    20581 tttattccga acaaaattga aataataatt catcacaagc gcactcatcg gcgccgccac
    20641 atcggaaccc tcgccgccgt tttccataag caccactata gctatttcgg gattttcata
    20701 cggcgcaaaa cctacaaacc agccgtgata accttcggta ccggaaccga ctttaccgtg
    20761 cacgttctcg gctgttcctg ttttaccggc gacctgcgcc gctttcaatc ccgctttttt
    20821 tcccgtacct tcaaaataat tgacgacacc gaacatcgct tgccgcatca tatccatgtg
    20881 ctcttgtttt accgccacat gtttgaccgg gaaggttaca cggcggatac cttcactatc
    20941 ctccgcatac cgcaccaaat gcggcgtttg gtaagtaccg gcattcgcaa aaatcatcgc
    21001 gtattgcaaa agttgcacgg gtgtcactag aatttctccc tgaccgatac ccagattgac
    21061 taccgcgccg cctttccaat tattgaaacc ataacggctg ttgtagtatt ctgtcgtagg
    21121 aatcaatcct cggcgttcat tgggaatatc cgttcctgta ggctcaccta gaccaaacat
    21181 acgcgcatat ttagccaaac ggttgatccc caatttccat gctaattcat aaaaataaac
    21241 atcgcacgaa cgcaccaatg aactggaaac attcatgtga ccgtgacctt taagattcca
    21301 gcatcgaaat ttattaccgc cgagctgata cacgcccgta cactccacat acgtttccgg
    21361 tgttaccaat ccctcttcca acccggccaa tgccgtgatc attttgaagg tagagccggg
    21421 cgcatacccg ctttgtacca tacgattaaa aagcggtttg tcgggatttt cattgagttc
    21481 gcgccattga tcaatcgtaa tgccatccac aaaccaacga aaatcataat ccggtttgct
    21541 ggtggccgcc aaaatttcgc ccgttcgtgt atcggccacc atgatcgaac cacgacgcgt
    21601 gcctagagcg ctttcgacaa atttttgata atcattgtct atcgagagat acagattata
    21661 accgtcgcgc gggtgtttgg atatgccgag atcacgcaga acttgaccct tggcgttggt
    21721 ttctaatcga tgatatcctt tttcgccacg caattcgact tcgtaaaact tctccaatcc
    21781 cgatacgccg acacgctcgc cgattttata gtcggtatcc tgaaactcgg ccgttttata
    21841 ataaccgata gtattatccg taatctggct catatagccg atcagatgcg gcatggtgat
    21901 gtcgtcggca taaatccgat tgatatctac tttatatccg atgccacgat gaaaacgtcg
    21961 ctcttcttca agatgtgcga tcgtgacaaa atccacttcc ggttttatta cgaccggcgc
    22021 gtagggccat gcgccgcgca gttttttgag aatgtcctca tccgtcatac cggtcagcga
    22081 acgcacatat tgcatttcgc cggtgctgag cgtgtcaaat tccgacaagg taatcgtcaa
    22141 tgcaaacgta ggccgatttt caacaatgcg ccgcccatat cgatcgtaaa tcaatccgcg
    22201 cgagggaatc tgatcaatcg tgcgggtgct gttatcttcg gcacgcgcgt gaaaattatc
    22261 ataagccatg atttgtaaat aaaacaaacg gccgagcagc atcagcaaaa tagccgctaa
    22321 cgaaacatac atggtaacga cacgatgatg attcatagtt tattaaaaat cataaatagt
    22381 gttgaaacga acatagtcca gtgtgcgctg gggtatggaa agataaatca cgaaggcggt
    22441 gacggccgaa tatagtgaat tccataatcc atagtgaaac atcaggctcc agaaatctac
    22501 gacgccgtcc agcgattgga tcgcatagaa aaacacggca tgaataaccg atagaaaaaa
    22561 aatcatgatc gggaaataat atttttcgat cagcatcacg cgttcggccg cgaagaagta
    22621 tgtcgcaaag gcggcaattg ttttacataa cgcattgagt ccgtaaaaat caatcatcgc
    22681 atcttgccaa agcccaaccg taaatccaaa taacataccc gggatctgcc cgaggcgcat
    22741 gcttacgaat accgtcaata tcagaagcaa atccggcaca ttatccccga cggccagaaa
    22801 aactgaaaca aaactgactt gcataaggta aaaaaaagcc gccgcaccca gtagcgagaa
    22861 tgtcaaaata tttcgtttac gacgcataga tcaacgcttt acgataaaat aattttcaaa
    22921 tcctacagcc accgaacgcg ttcgggacgt atccgttacg acaaacactt cttccaaacg
    22981 atcgaaatca acggccgttt gcacccggat atctttgaaa agaccttcga tctcgttatt
    23041 cacttccgta accacgccga cacgaaaatt gggaagaaaa tattcgctat actccgacgt
    23101 caggatgaca tcgccgatac gcacatccag atttttaaga acgccataaa atgccacttc
    23161 atccggattg ccgggccaaa ataccactcc gttggcacgg gtacgctgta tttttgaggc
    23221 aacacgaaaa tttttatcca acagcatctg gcaaacggaa tgctcttcaa atacttcggc
    23281 gatctggccg acaagaccgt attcgttgat cacattttgg tgcagccgta taccatcggc
    23341 agaaccttta tccaaaacaa tattactgat cgcagggcgc gactcccgcg caatgatgcg
    23401 tgcgggacgc acactcagcg gaaaccggct tcgaaattcg accaactggc gcaggcgtac
    23461 gttttccata tacgcatctt ccaaaacgat attacgcgac gccaattcga tattgcgctg
    23521 ttgtaattcc ttaataacgg catccgaatc gcgccaccgc ggcccggatg aaattttttc
    23581 acccagcatc gccgcaagat aaatcacgcg cttgcggaga aaaatcatcg atggattggg
    23641 attattctgt aaaagaagaa tagatgcgat gacaagaacg agaaaaacaa atgaatgccg
    23701 gtagacatac aggatctctt tcaggccggc ataaaagttt cttagtttcc agagcatgat
    23761 gggatcttaa tgcacgctac cggtacggta cggcgattag tatttgcgat ttttgagaat
    23821 gacggcctga tatttggtca ggttttccaa aacgcgtccg acaccgcgca cgacggcggt
    23881 cagcggatct tcggcgacga tcacgggtaa acccgtttct ttgcgcatac gctcatcgag
    23941 ccctttgatc aaagcgccac cgccggtaag aacaataccg cggtcaatca ggtccgatgc
    24001 caattctggc ggagtttttt ccagtgtact tttcgtacct tccacgattt cacgaatcgt
    24061 tccttccagt gcgcggcgta cttcttcgga atccgttttc atcgttttgg gaataccgga
    24121 tacaatatcg cgacctttga tttccatttt caattcctgc gcaagcggta cggcggaacc
    24181 gatattgcat ttgatatctt ccgccatttg ttcacccaaa agtaaattgt gctggacgcg
    24241 gaaatatgtg ataatagatt tagtcagttc atcgccggcg ctgcgaatcg aacggtcgca
    24301 tacgatgccc gacaatgcaa tcacggcgat ttcggccgtt cctccgccga tatcaatcac
    24361 catactgcct ttggaagctt cgatatctat acccacgccg atcgcagccg ccataggttc
    24421 cgccacaaga tatacttctt tcgcaccggc gtgttcagcc gaatcgcgca cggcacgttg
    24481 ctcgaccatt gtaatgcccg aaggtacacc gacaactacg cgtttaccca taaaccgggc
    24541 cgggctcact ttgcgaataa attcctgcag catcagttcg gtcatttcga aatcagcgat
    24601 cactccgtct ttcagcggac gcaccgtaag taactcgtca tgggtacgcc cttccatttc
    24661 tttggcttct ttgccgatcg ccatcatttt attggagcgc ttattgatcg caacgatcga
    24721 aggctcattg atcacgacgc ctttgtcttt gacatagacc aacgtgttgg ccgtcccgag
    24781 gtcaatcgca atttcattgt taaaaatatc aaacaggctc ataacgctcc gtatgcgtgg
    24841 ttttcttaaa tggatctaat gtttgaaatg acggatactt gtaaatacca tcgccatatt
    24901 gtgttcatcc gccgccgcga tgacttccgc atccttcacg gaaccgcccg gctggatcac
    24961 ggccgtagca cccgccgccg ccgccgcgtc aacgccgtcg cgaaaaggaa aaaaagcatc
    25021 cgacgcgatg gccgaatttt tcagcgaaag cccgcttttg ccggatttgc tgacggctaa
    25081 ctccgacgca tcaacccggg acatttgccc ggcaccgata ccgagagttc tgtcagccgc
    25141 agcataaacg atggcgttgg acttaacgtg tttgacgatc gtccatccga aaagcaacgc
    25201 gtgcatttct tccgcattgg gagcgcgtcg cgttacgacc ttccactccg tctgccccgc
    25261 acgacgcata tcggcgtcct gaatcaaata accatccaac gctttgcgga tctccggcaa
    25321 cgccgacccc tgcgcccgcg ccgcttcaaa atcataacgg attaaccgga tatttttttt
    25381 ctttgaaaga atctctattg cacccgaatc aaattccggt gcgatgatcg cttccacaaa
    25441 cacttcactt aaggcttcgg cggtcgccgc attgagacgg cgattaaacc cgatcactcc
    25501 gccaaatgcc gaaaccggat ctgtagccct ggcattctga tacgcctggt acaaatcgtc
    25561 gtgcatcgcg gcaccgcacg gattggtatg tttgacgata acgcatgatg gtttttcaaa
    25621 atccgataca acgcgcacac aggcatcaag atcgagaata ttgttgtatg acaattcctt
    25681 gccgtgcaac tgatcataat actttttaga gtgaaaatta gccgcgtaaa atgacgcttt
    25741 ttgatgcgga ttttcaccgt agcgaagcgg ctgaatttgc ttaaacgtaa gatttatttt
    25801 ttcgggcaac gtttccgaag aagcggttac tttagatgca aaataacttg aaatcaatgc
    25861 atcataacgt gccgtatgtt caaacgcttt gatcatcaaa cgataacggg tgtcttgcgc
    25921 cacatcgccg ctagtcatga tttccttcat gacaggttcg taatccgatg gatctaccag
    25981 tacgatgaca ctgcggtaat tcttagaagc cgaacgtatc atcgaaggcc cgccgatatc
    26041 aatattttcg atagcctcat gcaaaggtac atcgggtttg ctgatcgtcg cttcgaaagg
    26101 atataggtta accgccaaaa gatcgatcat gccgatgccg tggcgattag cctcttccat
    26161 gtgggtagcg ttatcgcgca acgccaaaag tccgccatgt atcttagggt gcagtgtttt
    26221 gacgcggcca tccatcattt cgggaaattg cgtgagatct gaaattttta cgaccggaat
    26281 tccgttttga gaaagcgccg acgccgtacc gccggttgaa ataatttcga caccggcttg
    26341 atgcagtttt tgagccagcg attgaattcc tgttttatcg gatacactga taagcgcacg
    26401 tcgaatcatc ttggaagcaa cacttctata aataaaatat aatttttcgg gcgcgccaat
    26461 gtagaaactg agcggttaaa atgcaaggtg tttagaggtg aaattcattt gataagagca
    26521 tggaaacata gacatactta ttgcaaaccc tctcattgat aaaacaaaaa aggaagagtt
    26581 ttagctcttc cttttttata cgattcctta tacctactat tgagcggtcg ccgtaaattc
    26641 gccggactgc aacactttac cggtgctgtc cacgatttcg actttccagg taccggcacg
    26701 gtgcacttca taaaacgcat acgtgcgcat tgaccaagat ttgatttcga gcggtacttc
    26761 atttttcaag gtttcgccgt gataccattt catggtaacc gtctggcctt caccacctac
    26821 aactttgatc cagcaatata cattgctgcc attggcggga aacgatgtag cttcgccgac
    26881 aacttcgctt ttttcgacag cagtgccaaa cttcataccg tcaactttca tctgcgaata
    26941 accggtttga acaaatgcca aagcgattaa aacgaccaaa gcacttacgt acttcatact
    27001 acacctcttt ttaaaattaa taaaataaat atcgcgagtt tttcccactt cgcatgccta
    27061 ttatacacga aagcgcttac gtggtcaata tttaaatata aaaacctacg aaaaaatcgc
    27121 aggttttaac ggtaacagac tattttttaa aattttatct cgatccggcg ccccattcgt
    27181 aatttagatc caaacgaaat gaaacctctt ttttatcttc ttccgaagga ttgtcaacaa
    27241 tcaaataata caatgttttt tgggcgcgaa attgattaag tagccgaatt ttaaatgtgt
    27301 gttgtatttt ttcgccggaa tcgtattgtg ttttgtaagg ttcttcttct ttaaatttga
    27361 gaaaattttc ttcgtccatc accatcacac gcgctttgcg atcgccaccg tcttcgacac
    27421 gccattcgcc atctacttcg atcgcttcca tcttatcttc cagtgtaatt tcaaattgat
    27481 aatactcacc ttttcccagt gtttctttct tttcgacatg tgtgatttta ctgttcatca
    27541 cggctttatt actaccgcag ccaaactgca tcacgaccgc caacatcgct atgccataaa
    27601 gccaaatttt acttaaacgc atacatttcc ctttctgaaa taataaaagt tattgacctc
    27661 gcatacatca gtatacctat tttgaggtaa acgatgattc atcctttata aaacatccat
    27721 ttggctaatt cgggcaatgt gattttttta ccgtacatga gaattccgat acgatatatg
    27781 cgaccggcta cccacacggc gctcaaaaaa ccggcaatca gcaagctcat cgaaagcgcc
    27841 aattgccacc aaggcacacc aaaaggtatt cgtcccaaca ttacaatcgg tgatgaaaac
    27901 ggaatcatcg acgcccaaaa tgccaccggc ccgtgcggat cattgaccga aacctgaagc
    27961 agaaaaaaag ataaaatcac cggtatggtg acgggaaatg ataacgactg cacgtcaccg
    28021 gcatcattgg cgcccgaggc cgcggcggcg tacaatgccg cataaaaaag ataaccgccg
    28081 agaaaataaa acacgaagca gaaaaataat tctgtaaaat taattgaatc cagatttttc
    28141 ataaatacgg ttatcgcctc cgcatcgcgg gctttgatac cgccttgggc aagtgcatcc
    28201 ggtgaaaact gccccatctg cggtcccagc agcagcatag ccccctgaaa cagtagcgcg
    28261 ccgatagcta tccatgacac gagctgagtc aatccgacgg cggcgacgcc gaggatctta
    28321 cccgttaaca tttcaaaagg ccgcacggac gagacaatca cttccacgat gcgattggct
    28381 ttttcctcca ccacaccatt catcaccatc acgccataaa tgataagaat gatataaata
    28441 atgataccca taataatcgc tgtcgtaaga gcgacaactt tattatcgga agactcacct
    28501 tcttcggtca taatgatcgt ttcaaacgaa acacgctgtt tgatcgcatc gatcttatcc
    28561 tttgtcattc cagcctcgga taatcgcgct ttttcctgtt cacgtgacaa ttcgctattg
    28621 atatataagg ttgtcgtaaa cccaagaccg cgggatgaaa aatattttat cccatcgttt
    28681 ggatcggtac tagccgcagg aatatacaaa acaccgtcgt aattatattt ggtgtcccgg
    28741 tacacacgtt tgagcgaatc taaaggcatg gattcgtaga aaaactgaac aagccgtgaa
    28801 tcctgcaatt tctgaatata taagccgctt tcgtctcgta cggcaatgcg ttcggcatcg
    28861 ttattgcgcg cgatcagaaa tatcgtcacc ggtagaagaa gcatcaaaaa aggtacaact
    28921 aatgtcgtaa tcaaaaacga tcgtttggtc acgcggctga tatattcacg ttttaaaaca
    28981 agtcctattt tacgcatgat tgtatccctt cacatgttcg atgaaaattt cattgagcga
    29041 cggtaaaacc tcctgaaatg catacatttc cacccggctc aggaggtgcg ttaataattg
    29101 attgggcgaa acgccgggtt gtgttgcgat cacggcttca cctgtttttt gcgacacgat
    29161 cgaataagcg gtatcgccgg attgcggtaa aattccgtca aactgtatgc ggtagtggtg
    29221 ctttttgaac tgttgtttga tcgctttgac ttccccgctg aggatattgc gacccaggtg
    29281 caccaatgca atacgatcgc atatttcttc gacttgctcc atacggtgtg tggaaaatat
    29341 aatcgtcgct ccttcgtctt tgagacgatg gatttcatct ttgatcagat cggcgtttat
    29401 gggatccagg cccgaaaacg gttcgtccag aatcagcaac tcgggctcat gtacgaccgt
    29461 cgcaataaac tgcacttttt gctgcatacc tttggataac tcctctacct ttttattcca
    29521 ccagtccatg atgtgcagac gttcaaacca catttgaact tttttttggg catcccataa
    29581 cgacagccct cgcagctgtg caagatacat gacatgatcg ccgactttca tttgtttgta
    29641 aagacctctc tcctccggca tgtatccgat acggcgcgta tgcgactcgt gcaaagcttg
    29701 gccgtcaaac cggatcgcgc cttcatccgg catggtgatc tgcgtgatca tacgtatcag
    29761 cgatgttttc ccggcaccat tgggaccgag taaaccaaaa atggattgac gttgtacatc
    29821 aagactgatg tgatccacgg ccgtatgcga ctgatagcgc ttgactacat cacgcacaga
    29881 aagtttaaca tccgtcatcg tcatgatttt tcctcgatag taattaatta tgtaaagtcg
    29941 tttctatatt ttgcaatgct tgtaactgag ccgtcatttg ttgtgcatct ttatcaaaca
    30001 tccagcgaac ataggggcga taaaaaaacg taactacggt gataaagccc ataaatccga
    30061 gtttgaaata catcatataa ttttcgttta cggcctccat cggtataaac aaaacaatca
    30121 atgcaagcac catactgaat ccgtatgccg agcgcagata gatacgaatt gttttttgat
    30181 gaagccgaag gtatgccgta tgacgtgcgc gcagatctat tccgtaatct aatgatgata
    30241 cacggcgaat cagaagaata aacaaaaagc cattgggtat gataaacaga aaggacgcca
    30301 cggtaatcgc caatgaaatt agtggtagat gcgattggac aaataacacc gcgccaatca
    30361 taaaaagaat ggccaacatt ccgctcaatt caaaaagaag tttctttcgg atacgaagtt
    30421 gcgccgttcg aatccgcatt tccagaagtt tacgaatatc tttttcattc ataccggttt
    30481 gaggataaag cgactgccct tgtttccaaa gttcacggag ttcatcgttt tccatgattt
    30541 ccggcctcca tgatttgttt gagttgtttc ttaatacgat tgatttttac accgacattg
    30601 gattccgtca aacccatgat caccgccata tcggcgtagc ttacctcctc gagatacaaa
    30661 acaatcagcg ctttttcgac gggttcgagc tgtgttatcg ccgactccaa tctccgtaac
    30721 ggttcagtgt cttcactttt ttccggggcc atattttcca gtacgccgtc ttccacccgc
    30781 tcggcgacga tcgaacgctg ctgcttacgt ttgtacatca gcgccgtatt gagcgccacg
    30841 cgatagagcc atgtcgaaaa tttggaagcg ccttcgaatc gtggatacga tttccatgca
    30901 ttgagcaaaa cttcctgaaa aagatcatcc tgatcatccg gcgtgtgcgc atacacccgg
    30961 catattttat aaatgatccc ctgatgcgta tgcaccagat cgagaaaagt tttagaaata
    31021 acgtccatcc ggcctttcgt tttgctcata ggttgccgca aagtgcgaaa acttacaagg
    31081 tttcaaaacg aaatcgtata aaaaagaaaa ccgggcatga ggaaacccgg tttatacgat
    31141 atattgcagg acgcagatat atcgcggata catatatttt aaggtaaatt aaggcgtacg
    31201 ggatttggtt ttgaaaaaac tcagcacggt ttccgtcgta ttaaaatcat taaaaaacca
    31261 ttggtgccct tcttcttccg gtgtcagcag tcggatttca tgacccgatt ccaaaccata
    31321 ccgatccaac gtagtatttt ttcctgcttt ttcgccgatt gccgtacgct gcattttata
    31381 ataattgccc caccacgtta atgtctgtgg cgcagaaagc agcgaaaaac tgccttcatt
    31441 ggaaccttga tacggtaaaa caccatcatt tttaccgtga atgataaaca ccgaagtttt
    31501 ttcttccggc gcaccatagg tggagagcgg ctgattcata gacgatgcca cactggcaac
    31561 agcggcaaaa cgatgggata aacggcaagc cacataatgg gcaaaaagcc cgccctgcga
    31621 aaatccgaca gcatatacac gacgcgtatt tacaggatac ttatctatca tcgttttata
    31681 tatcgcatca ataaaaccta cgtcgtcaat gccgagacgg tagggtttat tacaccggca
    31741 gccttcgtcc caattttcga ctttggcatc cggatacaca aagatacagg aatcactcca
    31801 cgtacttttg tcaaaaccgg agaacgaacg aatacccgcg ccgtttccgc cggcgccgtg
    31861 aaaaacaaaa acgagcggat atttttttcc atcgcgtagc atcggcaccg atgtgatata
    31921 tgtacgatcg gcatatgtcg aatcggtcac tgtttcagga tacaccggct tgagcggccc
    31981 gtcttcgcat gccgtcacca ttgcagccaa tgtatatatc ataagaagta aaggctggaa
    32041 catggctatt tgactcccgg tgtatagcga cgaatcatcg gaggtgcggc cttggttata
    32101 tccaattgat gatttttatc gtaagcgccc cacgggctat tggcgatata atacagcgat
    32161 ccgccggata cgcaccctag cgtaggttcg cccaaaaaat cggcattatt atctataact
    32221 tcgacggact tgattttact ccactccgga tcgcagctca accgcaggat gcgtaacgga
    32281 tttgtaccgt tttggatcgc atagatggcc gtaccgtcta aaagtaatcc gtcaatacca
    32341 cgcaacatcg acgacgatac aaatgatagt tttatcactt cacctgtttt gatattaagg
    32401 cgatagatac cggaaacata atcggcgatg aggatgtact gctgatcgtg cgttacatcg
    32461 acgccttgca aattccatac accatccatc gttttccatt ttactatttt tttcatatcc
    32521 gtatcaatgt gataaatctc cggcgcttta ctgtcgctga taaaaaccga acctgaagcc
    32581 gtaacacaaa ggtcaccgaa aatatgccca cccgacactt caaaccgagc gatttcatga
    32641 ccgcgatcgg catcaaaaca aacaaccgca gcacgtccgt caagtgaatc cgtgaaccct
    32701 gacatttccg gcatgaccga tgttgtcacc cataagcgac gacgcagtga atctatgcgc
    32761 atgcccatca ctgaatacag aggtttcgaa ggtttaaaaa aagtggtgtc caattttcca
    32821 tccgggcgca ctaccccgat taacctctga ctaatacttc cggtaatcca gagattgttt
    32881 tttttgtcaa aagtaatccc ttcgggatga aaactacggt catccagtgt aaaagccata
    32941 tcagatttta caattacagt ttggagtgat tcggctaatt gccgggtgga tacaaatcgc
    33001 gaatccgttt tgacggcatc aaaatcctcg tcttcataaa attttaatcc cgcattcact
    33061 tgcaaagccg atctaagcgg cccccatact ttatctgcct gatctgtcaa cgcatacccg
    33121 gcggccagat tatataatat accgtaatta tttggtcgca gagaatcagc acgctcgaaa
    33181 tgataaatca tttttgtgta atttttgtct tttgcggcag tggctgcttc tttgtagtga
    33241 aactgatact tctccatacg agtttgagca tacgccgcca ttgttattaa ccaacaaaaa
    33301 acaaaaaatg gaattcgatg cataaaaatt ttgattacaa tattatactt catacaaatt
    33361 gcttctttgt tttatttttg agattcaacc atttgtttca attttatttt tctgccgtca
    33421 gcgcccaact ttaaagcttt ttcccacatt tctttgtagt tatttgtttt attacttctc
    33481 ttgttctctg cattaatctg caattgggcg aactcctccc catataaatc aagccaaaca
    33541 tcatatagat ataagaataa taattcatga tcgttgaaat cctttgtacg acgttgcaaa
    33601 caaaaatgaa gcctatgtcc taactcatga atcaaagtac cttgcttgat aagagtaggg
    33661 taacttgatc tcaatcgcat tggcgtgcct gtcccacctg aattactggg catatcgacg
    33721 acattcacca ttacaagtgt atcaataaaa ttcatgccgg aaatacgctc catcgttcga
    33781 actatttttt tgctttcgga agaccatata gagtcgtatt catttttttc tgaaactgta
    33841 gttgaatcgg aagccataaa aatcaatttt agttttgtct gtccagcgat gtgcgccgaa
    33901 aaaacaagaa acgtcatgac taaaattttc ataaccagcc ttatcggata tatgaaacat
    33961 gaattgatca acctgatcac acaatatacg gtgactttat cactgtaaaa gatatcggtg
    34021 acgtgaaacg tcattttttt ggagggaatg gtatgtttat gaaggaaaat ccgattatat
    34081 tggtcgtatg attaatttat tgtatccctc atggttgtgg cgcatgacga ttgccgcatg
    34141 tatcggttta ttttcatatc ccgtgatcat gcttatccgc ggttatacga tcgaatcaac
    34201 cacaggatat tattatgccg gtttttcatt gtatgctgct tgtgcccatg cctggatgat
    34261 cgaatggcat ttatttaaga atcgccgttt gcggaacaag ctatcatggc ttgatcatac
    34321 ctggttacgt ttttttattg agcttccgtt ggcctttctg atcggagcaa caggggtttt
    34381 tatcgggctg tcggcgctta tgatgagttt ttttgacgcc tacaacctca gtctgacact
    34441 ctggtcattt tacttgacgt tgggtattct gatgtcgctg gtatattccg cattttttca
    34501 tgtcgccgaa ttattgcatg cctggcagtc cgcaaaagta cgtgccgccg aaatggagca
    34561 agcctccgtt cgggcacaaa tggcagcctt acaaaatcaa gtcagtcccc attttctgtt
    34621 taatagttta aatatggcgc atgctttgat cgatcgcgac acagaagagg ctaaatcatt
    34681 cattgaccaa ctcgccgagg tgtatcgcta cgttctgaaa aaccggcatg aggaactcgt
    34741 ttcagtacaa gaagaaatcg aatttgctcg ccattatctt gcgcttgctc aaatgcgatt
    34801 tcaatacatc ctaatcaaag aatctgtttc gccggatgcc ctgcaaaggg ccattcctcc
    34861 ggttacgcta caaattctga ttgaaaatgc cttgcatcac aatgccatta cgcacgaaca
    34921 tcctctgcaa ctttatatcg aatccacgga caactcgctc attttacgaa atacgcgaaa
    34981 tcctcgattg cacgctcctg accattccca cgccggaaca gggctagaca atattcggca
    35041 acgttatctt ttttttacgg aacgtgcgat acagattaat caaacggacg cgatgttttc
    35101 agtaaccata ccattattgg aatttcctgc atgaaggtat tgatcatcga agacgaaccg
    35161 gtcgccgcgg atcacgtggc ctcgttgctc cggcgtatta tgccgaacgt cgaaatcgca
    35221 ggacaaatcg caacggtgcg cgaagcagta cttatgcttc gtcaaaatcg ttttgatctg
    35281 atcttatcgg atatccaatt agccgacggg tcgtgttttg agattttcag acaaaccaag
    35341 gttaatacgc cgattatttt tattacagcc tacgatcaat atgcattacg ggcattcgaa
    35401 gtattcagtg tggattacat actgaaaccg gtaaccgaaa aaaaaatgga aacagcctta
    35461 aatcgattca aagaacgacg tgatttgttt tttgcagatt atgaaaaact tacgcagccc
    35521 tcgggtaaag aatataaaca acggtttttg gtgcgaattg gaagtaaatt gattcctgtg
    35581 gaatgcaaag acatactgtt tttttatgct gtagacaaat ggattgacct cataacaaaa
    35641 gacaataaac gttttgtcgt agacttcagc ctcaatcaat tggaagattt acttgacccg
    35701 aaggattttt ttcgcatcaa tcggcaatac atcattcaca ggcaggcatt gatctccatc
    35761 gctcagcatc aaccgggaca agtaacggcg cttatcaaat caaatccatc ggaatccccg
    35821 gtcatcagtc gccgagttac gccccgcttc aaactatggt tatccgaaaa taaaaaaccc
    35881 tgacggtgac atcaggattt atagttaaat atgatgatgt attatagcaa agttcggtta
    35941 aaatggtagg tcaagctaat gccatagtac cattgttttg acgacttcac gccattataa
    36001 gggtcaaaca gatttccgct attaacaaac cgtatagcct ccattgcacc catgtcataa
    36061 taaccattca aatcggatgc cgggctattt ttcttcttta accatgcggc atcccccgag
    36121 atacttaatc tgttttcgat aaaataggtt aagttagtgc taattttata tttcttactt
    36181 ttaaatgttt cgtgtgtatc ctcatcgccg attttaatat gtgcattatc gtatgtgccg
    36241 ctgacagtcc ataataaccg atccgcaatg gaatacagat gattgacggc caactgcatt
    36301 tgcttaagat cactgacagg gctatatttt tttgaacttg atcgaaaatc caaactacct
    36361 gttgccgaaa aatcaatttg cccattaatg gagtaattat gataaaattg cccgttgagc
    36421 atcattccta aataagtgga acgaaacttc acgtcttcat catccagata ataattgctc
    36481 gaatttctct tatctcggct ttggtagcga ataattcgtg gtccgacatc aatcacatag
    36541 ccttccaaac gatttccgat cgcttcctga aaaacatcgt tcatatagtg cgcttcaaac
    36601 ggcgttaaat ccggtaatgc ttcatctaac gagttccaga attctttttg atgcctatca
    36661 taaacattct gatacccata cagtcgtgca aacgcctctg caaccttc
//
LOCUS       DAOQGF010000021        35702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_526_length_35702_cov_6.674082, whole genome shotgun sequence.
ACCESSION   DAOQGF010000021 DAOQGF010000000
VERSION     DAOQGF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 35702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_526_length_35702_cov_6.674082"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="PK937_04960"
     CDS             complement(<1..1691)
                     /locus_tag="PK937_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH31951.1"
                     /translation="MKRFLCFAMCGLLCFTLKNVSAQQTWPVEGLHDKTPSIHAFVGA
                     TIIPGPGQKIDNGTIVIRDGVIEAIGKSVNIPREAVIHDLKGMWIYPGLIDAYAGYGI
                     APPAKEKGSDGPQPYTSKKGAYSWNQAVKPEFEAAREFTIDAKRAGELRALGFTTVHA
                     RPLDGIVRGTGAIVNLGESSASEEILSRQATANFSFDKGVSTQDYPSSLIGTIALIRQ
                     TFYDASWYGKAMNSKNRPDVNISLEALNTQFSAKLPLIFEVANTQDIFRVMQIGKEFG
                     QTFIIKSAGYEYERIEALKQNGVSLIVPVNFPSAYDVEDPDDARNVSLAQMKKWEWAP
                     ANPGALAKAGISFALTSAGLQDNKSFWTNIHKSIAMGLPADKALEALTTRPAKMLGID
                     AQVGTLEKGKLANMVIASGNLFEKNTTITQSWIKGRSYEVNRVPAIDPRGVWNLQGDL
                     KAYVLMINGTLSKLSGNFMYRDSQNVEVTPLINGDMLSIQFRGDSIGEPGLVRLKALL
                     INGRLSGTGEFPSGKTMSWSGTLFKSFEPKAEDNSIKSDSSVLSPISFPNMAFGL"
     gene            1897..2379
                     /locus_tag="PK937_04965"
     CDS             1897..2379
                     /locus_tag="PK937_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HNH31952.1"
                     /translation="MTKFKIVPLPLNLAERIRTDRMDDQGHTVVEQIASGYGPCRVSL
                     KPFKPGTDRRLLFSHSPFEVDNAFNQSGPVFIHAESVTPYEDIHRFPPEIKADKKHFP
                     LTLIGYSDDQRMVYTRLVGDRDVDQMISDIFEKHTDVAYLHARNAEAGCYICRIDRAE
                     "
     gene            2565..4070
                     /locus_tag="PK937_04970"
     CDS             2565..4070
                     /locus_tag="PK937_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH31953.1"
                     /translation="MNISSQTNSEQFKKEFEEFLKEKYGTADPEQDSAGTKNQGSTLK
                     SGEKTNFDLKPEELEEYLNQFVECQEEAIEVIATKIATHYNRMKLEDTLTDEQKLVGN
                     IKSNVLLIGPTGVGKTYIVKLIAKKIGVPFVKGDATKFSETGYVGGDVEDLVRDLVRE
                     ADGDIKSAEYGIIYLDEIDKIASAQNNRGIDVSRTGVQRNLLKLMEESEVDLRVPHDM
                     AAQMEAAMEAQRTGKVQRKKVNTKNILFVMSGAFGGIEENIRKRINQVRIGFGSDQEG
                     ISKLSRGELMKRVKTEDLVEYGFESEFVGRLPVIAVLNELTVDALFNILKNPNSSVIL
                     SKKRDFAAYGIEVEFDDDALHIFAERAFIQKTGARGLVNILENVLIKFEKKLPSTNIR
                     KFRVTKDVVLQPQAELNKLLTYSSMKEFQQQYLTTHGIIFEFSDVAIQKIAQRSQTEK
                     RSFRAIAAELFKDYEYGLKLAGKTHFLVTPEVFDDPRKYLDELIKKSYSNK"
     gene            4078..5364
                     /locus_tag="PK937_04975"
     CDS             4078..5364
                     /locus_tag="PK937_04975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH31954.1"
                     /translation="MKKVVIVDGVRTPYIKAGTLFNKMSAVDLGKLAMRELMARTNLD
                     SKLVDEVIVGNIGNPPEATNVARIIALEAGVPKHVPSFTVGRNCASGMQSIADAYLRI
                     IAGEADVIIAGGVESMTKVPLLFSERATNKFAAMMQARSMPARIGAMTSFRPGDFKPV
                     IGLLLGLTDGFCGLNMGETAEVLAKEYGITRKEQDELALLSHQRATTATDNGILKEEI
                     MPVYLPPDFKKIADEDNGIRKNQTMEALGKLRPAFDKKYGTVTAGNSSQITDGAAFVL
                     VMSEEKAKALGYTPLGSIRGFGFAGLDPERMGLGPVYATPVALKKAGLSMKDIQRIEM
                     NEAFAAQVIANERCFASKKFAEEKLGLTEAVGEIDRQILNVNGGAIALGHPVGSSATR
                     LVLTVLKELKRKNLQFGLATLCIGGGQGGAMIVEHS"
     gene            5377..6525
                     /gene="moeB"
                     /locus_tag="PK937_04980"
     CDS             5377..6525
                     /gene="moeB"
                     /locus_tag="PK937_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNH31955.1"
                     /translation="MYGLTTEELARYNRHLILKEFGLEGQQKLKSAKVLVIGAGGLGA
                     PCLLYLTAAGVGTIGIVDFDTVDASNLQRQVLYTVDDVGKPKADTAAHRLKQLNPSVH
                     FNTHREKLTSANALDIIRSYDIIADGSDNFQTRYLVNDACVLLNKPLIYASIFQFEGQ
                     VSVFNYTDPSGTLGPNYRDLFPTPPPPGMVPSCAEGGVLGVLPGIIGSMQASEAIKVI
                     TGIGTPLSGKLFLWNALTFESRTMKFKRDPANPINGLQQTITALIDYEVFCGISEPEK
                     QTGGEISPAELKHWIDTGKDFQLIDVRESFEYGICNLGGTLIPLAQIMQESARIAQNK
                     PVVVHCKMGGRSAKAIAQLRTLGFDNLINLRGGIIAYAETVDPSLAKY"
     gene            complement(6530..7993)
                     /locus_tag="PK937_04985"
     CDS             complement(6530..7993)
                     /locus_tag="PK937_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH31956.1"
                     /translation="MLSLLIIKEFKNILQSPKFIATFISCTLLIILSVWVGIREYKLA
                     VKDYETGIALNRQDLSERTNWMNVGNRVYRKPDPMQIWTFGVHYDIGRVAHVNTWDSP
                     ELRSSIYSRDLVYAVFRFLDFNFIVVVVLSLFAIVFTYDAVSGEKESGMLKLTFSNGV
                     PKSTYIVAKIIGSWAGLGIPLIVPMLLSFLMTILFQIPLTPDHYARLGGLILAGALYF
                     TFFIILGILISSWTSHSNASFLTLLVLWVTITFVVPRLGVMLSGQVLPVPTAAEVDSQ
                     IEGFSKSQFETLRQNLSETWMKRNNDVQQQPESERQTYRDKMEWTWLEEDQNARKSVQ
                     ENIESFTRKVHEDMDNRKREQIKLGLIFARWSPTSLFQLAAMRFSQTDIFLKERYDDA
                     IRTYQKDFSDVVEKKRRENRAEGLRIEVDSDRGFRVQTPDLKKTIDVRSIPEFTHPAV
                     SAGHAWRLSVWDIVLLAVITGFVFLGALVRFLRYDVR"
     gene            complement(8006..9418)
                     /locus_tag="PK937_04990"
     CDS             complement(8006..9418)
                     /locus_tag="PK937_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH31957.1"
                     /translation="MFTLLLKKEIRDILNSPKFSALFGMGSLLILLAFYMGARNYQVL
                     QNQYESAVRENLRQLEGVTDWSRVEPQIHLPPSPLASLISGVSNDIGRSVQVQAGAAE
                     LVPDHTRYGEDPVYAAFRLLDLEFVFQLILSLVAILLAYNAVNGEREQGTLKLMMAHA
                     VPKDKVIFAKLVGSFTATALPLLVPILIGCGILMVMNVPLTGAEWLRLMLIVCSGMLY
                     YFVFLSVSILVSSLTARSAHAFLALLIIWIFTVLIVPRTAVLLAGRAIEVPSVDEINS
                     QKFRHQSQLFNEHFQKIGEFKPNSNDPEKAMNEFNEFMDKARADREKKVVEFNERLNE
                     DRDNRQKIQQQWAFALGRISPATSFSLAAMQLAGTSSELRNRFEESVKSYKQTFKQFV
                     FEKTGKAPGGNMMMIRIGDSPEEKPKPINPYDLPQFVMPSLTFSEIINAALPDMAILI
                     VINMLIFAATTAAFLRYDVR"
     gene            complement(9444..10826)
                     /locus_tag="PK937_04995"
     CDS             complement(9444..10826)
                     /locus_tag="PK937_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH31958.1"
                     /translation="MIKLFFMVWFISSLTVAQSRDVQVSGLDSLIHSAVRDGFHGLVW
                     IESNGKPIYYKGFGFANESNNQKFDSTVFVQIGSTVKDFTRVAIYQLIEKKLLSLDDP
                     LSKYISDLAGEKNKITVQHLLNHTAGFPIGIKFDAEPLTKEEMVEHIRGLNLLSSPGV
                     HEKYSNLGYSCLAYVIEKVSGRSFDAYVDEYILKPLGMHHTGTYRPNFDRNKVAHGYR
                     QNADIGIILDMPHDQDGHLWSLRGNGGYLSTFRDMKLFFDSFETGKLLSTPAFRMAVY
                     DSKQPTVLAGSDMISFYGFANFPGIKARLMIASNHDNYMGHQLLESIEGYLRNGKNPM
                     GKVIKTVEVDNPGESAKVFDLPKEGAGLTIRRYVEAFNSGNSNTMKAFFENYAKSGDG
                     FAPMERRLQNYNRLFSDLGVITVNSFQTRADGSWEVHVGSAKAKAIFNFQIETKAPWR
                     FEALQIELGD"
     gene            complement(10850..11599)
                     /locus_tag="PK937_05000"
     CDS             complement(10850..11599)
                     /locus_tag="PK937_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH31959.1"
                     /translation="MTVHNQPVLEAAQLTKRYDDGALALNGISFQVHPGEIYAMLGGN
                     GAGKTTTINIFFNFITPTSGEAKVKGIITHQEPLRAKEQMAFVSENVMLYPNFTAMEN
                     LEFFAKLGGRADITESEMKVTLSRVGLQSEAMNKKVRTFSKGMRQKCGIAIAIIKKAP
                     AIILDEPTSGLDPKAGYEFNRLLIELKQEGKAILMSTHDIFRAKEVADHIGIMNRGSI
                     VMQKNRDELAHEDLEKLYIEYMAGYMEPVAV"
     gene            complement(11608..13098)
                     /locus_tag="PK937_05005"
     CDS             complement(11608..13098)
                     /locus_tag="PK937_05005"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNH31960.1"
                     /translation="MLRWFYFTILFFCFTDSGLMAQYNAFNQRDDKYPLLGLKRAKEV
                     YESEKAEYERVKQLFDKQLASPAELERARRSLADVEVNYQQSLLAVLFEQQFITVSRA
                     VKYQDKRGQKRVRVELMNTSGGEAEFRKLISMEDELFRSLQPDHINNIYVSVTNTEGA
                     IISQPYESKVNELVYGQPVTLDFVMLQDLDVVSVNIIYGNGASRSMRIFLQKDATENK
                     VLFQSEQFSQEADLGSNATFDLTLELFSGTDNTFKLEVLNLPPELTRYFLDAATNAKL
                     SQLKFAESARTRKASLRVFLPDRPTEQVQIEKTIPFYVLAIPQEKQALIQTQRDKVWS
                     QQEIESLNAGYLRLELIVKGNARLLVKAPKLYYATTESEPVEASLEIRNDGSRRLDNI
                     KFEVDLPLEWKKEIQPLMIPSIHVNEESVVRFKFYPPEHVAVGKYEVRIRTSSVSDNQ
                     PVNAEDKVITIDIQAGSNALGTMALIVLILSIIGGIVWFGIKLTRK"
     gene            complement(13098..13532)
                     /locus_tag="PK937_05010"
     CDS             complement(13098..13532)
                     /locus_tag="PK937_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNH31961.1"
                     /translation="MKKIILILGMTLFGVGGNAFAQGIRYMATGPVQPVDEVGVIFAE
                     KENGLIADLIFPEHALPKGYKPQGIEKNDVLVMVNGVSVKTANELKAAYDKVKVGDTI
                     KLGLKRGDEKMIASFAKIDPEHLPKRKMIKRTPDGKEIEETK"
     gene            13699..15480
                     /locus_tag="PK937_05015"
     CDS             13699..15480
                     /locus_tag="PK937_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH31962.1"
                     /translation="MAKSKRLHPGWIIALLTIALISIGTMQYIWLGRLSEGELTRMRS
                     NLHISATRMASDFDSEISRLHEYLFRVDFDQPEQLPNILQTTVNKWQTNAARQDLIRS
                     VFYGRMSENNTPEVFLLDSARPELHRVSDTVSNPIVLKLQRMRAMRSVGKEKNNLIIM
                     QGHLLADYPYIIVTVNPMRKMLNLPWEQPAEEIIAVLLDTNAVKSWMDSLAGIHFGAS
                     FENDVDIAVINDDQSKFFYKSNSNLPIAHWSKSDISAEIARIRTQDMFMVRVKGTRDK
                     TTMKTLTVTQSIETSERTSNGAFNPIIDSIVTQLHADTKTSGCNLRVIHREGSLEDAV
                     KIVRWRNLFLSAGVLLIMASGLVILLIAQRKERILANQQLEFVAGVSHELKTPLAVIR
                     SAGENLSDAIVTDADDVRRYGELIRGESKRLSDMVDQILDFSGIITDKNSLKMERLDL
                     SIIIQKCIASFPNDTPIYFEDRQTTHNMIIGNHEALQTIFNNLIANGIKYNRANAPIR
                     ITLTDESPYIRTDITDCGIGIDKKDIPHIFEPFFRASGVRDAQIHGNGLGLSIVERYV
                     ALHSGTIRVESEPGKGSTFSVFLPEGT"
     gene            15477..16163
                     /locus_tag="PK937_05020"
     CDS             15477..16163
                     /locus_tag="PK937_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH31963.1"
                     /translation="MKKLLLVEDEPGLIITLSDRLRREGFVVDIAENGTIALSKARKN
                     RYDIILLDVMLPQSSGLDVCRDLRGDGDRTPILMLTAKSQTTDKVIGLKLGADDYLTK
                     PFDMSELTARIEALLRRSESANRMEASDNYSFNNIEVDFRKAEVRVHKKTVELSAQEF
                     RLLRYMIEHRNATLSRDELLENVWNYGEDITTRTVDVHMAWLRQKLEPNPKRPQYFIT
                     VHRLGYKFVG"
     gene            complement(16145..16603)
                     /locus_tag="PK937_05025"
     CDS             complement(16145..16603)
                     /locus_tag="PK937_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNH31964.1"
                     /translation="MMKGIAELSGYPVVYSTPVAWGEMDALGHVNNIMYFRYFESARS
                     DYFQRIGVWDHAREHGVGVILHSTQCRFKRPLTYPDQIHVGATTVEVMADRFRMKYAV
                     WSEAQQAIVAEGEGLIVVYDYNQNQKCPMPSWMREAIAELEKKASQPTNL"
     gene            complement(16730..18502)
                     /locus_tag="PK937_05030"
     CDS             complement(16730..18502)
                     /locus_tag="PK937_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNH31965.1"
                     /translation="MKRFFITAFLFTSLLSCGKTLQEEADAFLKSYNQEYQAVYYEAS
                     KAQWKALTDINALNDSLSVLANKKYAAFAGSKDIIQKVRFFREHRSELNDITIRQLDK
                     IWLDASHQPGTMPDVVDQLIKANTEATSALYSYQFTVNGKPYSANDIDRALRNSSDLK
                     EREAVWTASKEVGKIERDHLARLQELRNKVAREMKYSSFFDLEAHDYGMSSDEVIQLM
                     DQFSEDLKPLYRELHTYIRYELAKKYNQPVPEYLPAHWLPNRWAQNWPGLVPGLQGEG
                     DIPGKSKEWVVEQAEKFYMSMGFEKLNDNFYKNSDLYPADAASGRKKNTHASAWHLNL
                     DQDYRSLMSVEPNMEWFKTTHHELGHIYYYIAYTNPEVPLTLRAGANRAYHEAMGDLM
                     AVAASQEAYLASLGLFDKTKTPDAIKLLLNSALSNSSIVFLPFAAGTMTHFEYELYEK
                     NLPKEEFNKTWWVLAKKYQGIVPPKERGAEYCDAATKTHIIDDPAQYYDYAISCVLKF
                     QMHDHIAKQILKQDPANCNYYGSKEAGEFIRSIMRPGASKDWRQVLKEKTGQDLSAKA
                     MLDYYGPLYDWLLQQNKGRKKAEF"
     gene            18628..19110
                     /locus_tag="PK937_05035"
     CDS             18628..19110
                     /locus_tag="PK937_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNH31966.1"
                     /translation="MKRVLKWLAIGLPVLFIAIQFIRPERTNPPIDPERTLEKHVTIP
                     DDIKTMIKTACNDCHSHETVWPWYSEIAPISWLVAHDVNEGRRHLNFSDWKKYNAHRQ
                     VEKLNEMAGEVRSEKMPMPIYLITHGDARWTKEDRKKFQNWAEDAADALSENLPDEDK
                     "
     gene            19192..20520
                     /gene="ahcY"
                     /locus_tag="PK937_05040"
     CDS             19192..20520
                     /gene="ahcY"
                     /locus_tag="PK937_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNH31967.1"
                     /translation="MSVAEKLNVPAYVPYKVKDMSLAEWGRKEIKLAEAEMPGLMALR
                     AKYGVTKPLKGARIAGCLHMTIQTAVLIETLVELGADVTWSSCNIFSTQDHAAAAIAA
                     AGIPVYAWKGMNAEEFDWCIEQTLFFGKDRQPLNMILDDGGDLTNMVLDKYPSLAKGV
                     RGISEETTTGVHRLYERVKKGTLPLPAININDSVTKSKFDNKYGCRESLVDAIRRATD
                     LMLAGKVAVVAGFGDVGKGSAESLRDAKCRVIVTEIDPICALQAAMEGYEVKKMDTAI
                     KEADIIVTATGNCRIVTDRHFRAMKDKAVLCNIGHFDTEIDMAWLNKNYGHTMDTIKP
                     QVDKYTVDGKEIIVLAEGRLVNLGCAMGHPSFVMSNSFTNQTLAQIELWNNSAKYENK
                     VYTLPKHLDEMVARLHLEKIGVELDELTSEQAEYIGVDVKGPYKPEMYRY"
     gene            20603..22423
                     /locus_tag="PK937_05045"
     CDS             20603..22423
                     /locus_tag="PK937_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNH31968.1"
                     /translation="MKSFTELLNEKIIVFDGATGTNLQTQNLTADDFGGEAYNGCNEY
                     LLISRPSAMEKVHVDFLEAGADVIETDTFGSSSIVLAEYDLQDRAYELSKLGAALAKK
                     CALDFSTPDKPRFVAGSVGPTTKLPSLSHIDFETMRASFYEQLAGLMDGGADLFCIET
                     CQDILQTKIALCAAQDLFRERKRKLPVIVSVTVETTGTMLMGTEISAALTTLEPYDIV
                     DVVGMNCATGPQEMSDNIRFLCHNSPKHVFCMPNAGIPENIGGKAHYHLSPDDLVKWL
                     MHFSKDFGVNVVGGCCGTTPAHIKPLAEAAARLNPLKRTWNYTPSVASIYMSSPMHVE
                     PAPLLIGERTNANGSKKFRELLIAENYDEMVSMGKEQTREGAHVLDVCVAYVGRNEVR
                     DMEETIRRFNTQISIPIMIDSTEVPVIERALQMLGGRAIVNSINLEDGEERTDKVLSL
                     CKRYGAAVVALTIDEDGMAKTRERKLAIARRIRTIAVEKYGMRDEDLIFDTLTFTLGS
                     GDEEFRKAGIETIEAIRLIKQTYPGCKTLLGVSNISFGLSPQARHVINSVFLHYAIEA
                     GLDMAIVNAQKIMPLYKIDPHERELARQLIFDERKWEPAA"
     gene            22420..22695
                     /locus_tag="PK937_05050"
     CDS             22420..22695
                     /locus_tag="PK937_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31969.1"
                     /translation="MIVPIFRVLSILCGLFFLMTGWIWQYLMALFVLPVGIAGMILWF
                     SANRMAKKLPDENETTARWLNQAAWWINITAFLSSGVALLYNLVAVR"
     gene            complement(22692..24296)
                     /locus_tag="PK937_05055"
     CDS             complement(22692..24296)
                     /locus_tag="PK937_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH31970.1"
                     /translation="MSCVPAQQEERITVAVRTEPDAINPVTHTSAFSRIVCTGILPRL
                     LESEWDSLQGRAVYKPFLAKSYSFSDDRRVLTYHLRSDFFWEDGERVTAHDIRFTFQM
                     IAHPDVASTKKEHIQYLTDALGSVDRSVIALNDSTIEFHFTRAYPLQIFDTNLQPGFL
                     PFHILKDVKPSELRRHTINTMPLSGSYYDITIWKKQDYIVLERNARSPYQAASRQIVF
                     RIIPEYTTRLTALKTGDVDVMYPIMPQDVADLRKTNPELRIEAMRSRYYDYVGWQNID
                     QHAYHASSGNTIQPHPLFGSKHVRRALTMAIHRQEIVDGFMGEFGRVAITPISPVFRW
                     AANDTLSPLPYEPEESKRLLAQEGWIDRDGDGIVEKNGRAFTFELYYDAGNDRRQFAA
                     LIVQRDLKKIGIRCDVRTAETNVFYDDELNKKYDAFISGIGVPLMIDPTSEWSSDLKK
                     NPYNDISYQNPNVDALIEAGKRVNNPEDAAPMWRALQAILHEDQPATFLYWRDELVGY
                     NQKIKNSRTSLLGEIDKFWLWEKVTN"
     gene            complement(24338..25723)
                     /locus_tag="PK937_05060"
     CDS             complement(24338..25723)
                     /locus_tag="PK937_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH31971.1"
                     /translation="MSVLGTAENPLRVAVIGSGPSGFYAADHLLKQKDVIVKIDMYDR
                     LPTPYGLVRGGVAPDHQKIKSVTKVYEKTAAHPMFSFYGNVELGRDVTRQELKTRYHA
                     LIYAVGAQSDRRMGIPGEDLNGSHPATDFVAWYNGHPDYRDLSFDLSQERVAIIGNGN
                     VAVDVARILASSIEELNRTDIADHALVALKKSNVKEIYMIGRRGPAQAAFTNAEIKEL
                     GELEECAITIPGTEGALDEVSKKNLEAHPDSGVSKNLEIMKDYLDKTTDGKRKKMVLR
                     FLWSPVELKGREGVEEIVLQKNILVQDADGSIKAKGTEAKETIPVGLVFRSIGYKGVA
                     LKDVPFDEKKGIIPNAHGRVTELNGTVIPGEYTAGWIKRGPSGVIGTNKPDALESVNM
                     LLEDVKSGHIHPIDQAASTTDWLQSKKIRFVTFADWLKIDKAETEKGMASGRPRIKFT
                     RIEDMLHIAGV"
     gene            complement(25845..27020)
                     /locus_tag="PK937_05065"
     CDS             complement(25845..27020)
                     /locus_tag="PK937_05065"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNH31972.1"
                     /translation="MKEVVIVSAVRTPIGSFQGALSEIVATKLGAIAVAEAVKRAGIQ
                     PTDVDEVIMGNVLTAGEGQAPARQAALFAGLPESVTCMTINKVCGSGLKAVMLATQAI
                     QCGDADVVVAGGMESMSNIPYILEKARTGYRMGHGQLVDAMIKDGLWDVYNNFHMGSA
                     AELCAKECNIPRDAQDAFAKTSYERAQKSIKEGVFKDEIVGVTVTSKKGDVVIADDEE
                     PGKANFEKMLTLKPAFQKDGTITAANASKINDGAAALVVMSREKADKLGLKPLVRIVA
                     QASSARKPEWFTTAPIDAIGKVLQKANQKIEDIDLFEINEAFAVVSMAAANQLNIPSE
                     KLNVHGGAIALGHPIGASGARILTTLIHAMNRHNKKRGMAAICIGGGEASALIVERI"
     gene            complement(27096..28127)
                     /locus_tag="PK937_05070"
     CDS             complement(27096..28127)
                     /locus_tag="PK937_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNH31973.1"
                     /translation="MPHTEERSIKIKGISPLVLLGPNDINLKILERAYTARIVVRGEN
                     ITLRGDASEVHQLEEIFTELIFLANKKSRLDSVDVETVIRLVKTGRDEHSSESDGVIT
                     HKNLNAVVLFTHKGFIEAKSDIQKSYIREARQNDIIFAIGPAGTGKTYLAVAIAVAAL
                     KNKEVDRIVLSRPAVETGESLGFLPGDMHEKIDPYLRPLYDALHDMIPSNKLQDYMAH
                     HVIEIVPLAYMRGRTLSNAFVILDEAQNTTPTQMKMFLTRLGANARAIVTGDISQSDL
                     PRGMMSGLREVKEILTGIEGISFVYFDKNDVVRHKLVRDIINAYDAYDDEQTGAIHRK
                     NLSIKSSNE"
     gene            complement(28218..29090)
                     /locus_tag="PK937_05075"
     CDS             complement(28218..29090)
                     /locus_tag="PK937_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH31974.1"
                     /translation="MKIKSLLALGLSILITGATVQAQDEVKMEKADWAAQVAAQQQQQ
                     AAQQRTIDSLNKAINGVKEEGTKVDQETEQVWQEIYAALGTDKAGVEAYKKQIADLEA
                     KVAEQSKLSDEALSKPEKQKELEALKAETMALKKDKRYALTETYNKVNDLNSRVDQVI
                     SRGKNYTPPKPRHDSYSVVMGDYLWRISGKKEVYSDPFQWVKIYSANREQIKNPDLIY
                     ANQTFIIPRSEEANEYWVQRGDNLKSIAQSRLGNPSDWWKIYNANKNVIDNINKGADT
                     KVIYPHTILIIPKN"
     gene            complement(29116..29388)
                     /locus_tag="PK937_05080"
     CDS             complement(29116..29388)
                     /locus_tag="PK937_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31975.1"
                     /translation="MLKSKALLVAGLFALLTMASCKSYATREELQQLDDNKAKTKSME
                     NKVVELTNEKKRVEQEVQTKKQKLEQAKKEKSEVLKRLEEIEKNNK"
     gene            complement(29739..32726)
                     /locus_tag="PK937_05085"
     CDS             complement(29739..32726)
                     /locus_tag="PK937_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH31976.1"
                     /translation="MLTGRQKIIYTNNSPDTLHALHMNLYVNAFRKNSRMHRYHEERD
                     KYYGGSLISYVPEAYLGYSKVQNIRDEPGRLLSQREDDTLLEITLAEPIQPGGVCTLT
                     LDFEVKIPAQIRRMGRFNREGVSFSIAQWYPKLCVYDHRGWHKNYYLAREFFGEFATW
                     DVSITLPAHYIVGASGHLTNAENIHAMMMSEPESTSMDRVLSSNGNRDSIPEGFESVQ
                     RKLAEITRQQSVTMPNRTWRYHAEEIHDFAFAADPDYKVEIFDHRGMRVNLLCLPDVE
                     QRWAPMKEWVTKIIDYMEAHVGRYPYPELTVAQAGDGGMEYPNIVFITGRRGNFSLAS
                     VTAHELIHNWFYGLLANDETHEAWFDEGITSYYTTRLMEHLFGRYANREYETEFQKKW
                     FPREDARIATFTGWAHWVKQGYEEPVLTHSDYFKSDQSYSMATYYKGQIFMNALEYRY
                     GRERLDSLMRDFFEVYQKHHTLTDDFKRFLEYKTGEKLDSLFDQWLNTTTYCDYGIAH
                     INNSKNTAGQNQVKVKLVRHGAISMPVDLYLKLTDGSWSGVRIPARHDDPNKSGLKRW
                     TEWPASDPTYTLTMITDLPVDRAEIDTSGRLPDIFRMNNRSGFLPKTEWHWQRPLGIL
                     PTSDTYVIEHRPSLWYNQTDAARIGWKMRGKWATDEHQIKAGVYAGTRSRTIDYEITY
                     STPMYSIGRQVFVDMASYKLDGVFQNELSFRKKWFGGAYERPPYYDVSLGLRTSRRFN
                     SAYLNPGVFWEKGDVHVLTASLQWQPQIFDSPRLKMTVETSTFASDYTFTKADLTYYR
                     PWVLKRNVATLESRIFAGFADGNVPVQDQFYLAGASPRQMFFNPWYRSRGTLPEKGWR
                     DASGQRHIYYDGQGSMAGYADSNALGMHIIAINLDLKPANPLKPIIKKEILFITQFEP
                     ILFFDAGGVSGKSGKRRHSIPSLVFDAGCGFRYLPAFIPSWMGNYAIKAEFPLWVSDP
                     SLAGQSGKSWAFRWLIGFENTIR"
     gene            complement(32909..33583)
                     /locus_tag="PK937_05090"
     CDS             complement(32909..33583)
                     /locus_tag="PK937_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH31977.1"
                     /translation="MEGIQTTVKFAGPGDSVAVIKVGGYIDTTTSSELERTLGSLLKK
                     NQHRIVVDLGDVDYISSAGWGIFISEIKGIKENSGDLKLVRMIPEVYEVFELLEFHYI
                     LKAYDTLDEAVDAFVKESGGSMSAKPKDSTQTIQAASVAKPVTAAPVTKQASKPADEA
                     EDEEDNTPATTQGDETPDEYLARAVRQYPTLSAWQLKKVLMTSDFGFTNMGWFEIRKK
                     MKSVRR"
     gene            33833..34831
                     /gene="galT"
                     /locus_tag="PK937_05095"
     CDS             33833..34831
                     /gene="galT"
                     /locus_tag="PK937_05095"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNH31978.1"
                     /translation="MSELRKDPIVNRWVIISEERGKRPSDFAETPSQLTTSCPFCAGN
                     EHLTPSEIMAFRHEGTMKDQPGWSLRVVPNKFPALMVEGQESHRHNGFYEFMDGLGAH
                     EVIIESPQHLCTLEEMSAPDFTNVIRAYRDRIRDLRNDTRLKYALIFKNQGAPAGATV
                     EHTHSQLIATPVVPKTVEEELRGSQDFHQTHNQCVFCGILNDDRKDNQRIVLDRDGFI
                     TLEPFAARFPFETWILPATHEVRFEDMNDEKINALASVFQETLMRLRKALNHPPYNFV
                     LHTAPLNTYVPDHAYHWHIEIIPKLTRVAGFEWGSGFFINPTAPENAATYLRQVRL"
     gene            34849..>35702
                     /locus_tag="PK937_05100"
     CDS             34849..>35702
                     /locus_tag="PK937_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNH31979.1"
                     /translation="MKKSKIFFVAPAVYPFARDGRVAEFAAGLPAQLQSMGHEVRVLM
                     PKYSLINERKHIIRDIIRMKDIPVKMYNGDLPVSVKSGFIPETKTQIYFAEQEKYYKR
                     SDLYRDKRTGEYYKDNDERFILLARTAIETLKTLGWQPDIVHCIDWQSGLLPAMIREE
                     QKQNNFFKKTIITFSVINTEEPALFDKSVFDKAFGQAHIIEKEKCFHKSKFSFLKTAI
                     MHSDAVTIPMTHKHLKTITKPANDVEAILASAKMVQESYFGGEHNYWSPVNNNTLYKS
                     YSAEKLER"
BASE COUNT         9567 a         8677 c         7795 g         9663 t
ORIGIN      
        1 ttcaaaccga aagccatatt gggaaaactg atcggtgata atacggacga atccgacttg
       61 atcgaattat cttcggcctt cggttcaaac gatttgaaca atgttccgga ccagctcatt
      121 gtcttacctg aaggaaattc tccggtgccg gacaggcgac cattgataag cagcgccttc
      181 aaccgaacaa gtccgggttc gccgatcgaa tctccgcgaa actggataga cagcatatcc
      241 ccgttaatca aaggagttac ctctacgttt tgcgaatcgc ggtacataaa attaccgctc
      301 aactttgaga gcgtaccatt gatcatcagc acgtaagcct tgagatcccc ttggagattc
      361 catacgccac gcggatctat cgccggcacg cgattgactt catacgaacg ccctttgatc
      421 caggattggg tgatcgtcgt atttttttca aataaattgc cggaagcgat taccatatta
      481 gccaattttc ctttttccag cgtgccaacc tgtgcgtcta taccgagcat tttggccggc
      541 cgcgttgtca gggcttccag tgctttatcc gccggtaatc ccatcgcaat agatttatga
      601 atgttagtcc aaaatgattt gttgtcttgt aaaccagccg aagtcaatgc gaaggagata
      661 cccgccttgg ccaatgcgcc gggattggcc ggagcccatt cccatttttt catctgggct
      721 aaggagacat tgcgcgcatc gtccggatct tcgacatcat acgctgaagg aaaattgacc
      781 ggtacgatca atgatacgcc gttttgtttg agcgcttcga tgcgttcgta ttcatatccc
      841 gcggatttga tgatgaacgt ttgtccaaac tctttaccga tctgcatcac ccggaaaatg
      901 tcctgcgtat tcgcaacttc aaaaatcaaa ggcagtttag ctgaaaattg tgtattgagc
      961 gcttcaagag aaatattcac atccggacgg tttttggaat tcatcgcttt gccataccac
     1021 gatgcgtcgt aaaatgtttg ccggatcagc gcgatcgtac cgatcagtga actcggataa
     1081 tcttgtgtag aaacgccttt atcgaatgaa aaattggccg tcgcctgacg acttaaaatt
     1141 tcttcggagg ccgaagactc gcccaaattg acaatcgctc cggtgccgcg cacaataccg
     1201 tccagcggtc tggcgtgaac ggtcgtaaat cccaacgcgc gtaattcgcc cgcacgtttg
     1261 gcatctatgg taaattcccg cgccgcttca aattccggtt taacggcttg attccaactg
     1321 taggcgcctt ttttggatgt ataaggttgc ggaccgtcgc tccccttctc ttttgccggc
     1381 ggtgcgatcc cgtaaccggc gtacgcgtca atcaaccccg gataaatcca cattcccttg
     1441 agatcatgga tgacggcctc tcgcggtata ttaacggatt tgccgatcgc ttcgatgaca
     1501 ccgtcgcgga tgacgatcgt gccgttatca attttttgac cgggcccggg aataatcgtg
     1561 gcaccgacaa atgcatgaat cgaaggcgtt ttatcatgca gaccctcgac cggccatgtt
     1621 tgctgcgcag aaacattttt caacgtaaag cacaacaaac cgcacattgc aaaacataaa
     1681 aatcgtttca tcacacctca ctgggttatg gtaaaagaaa tacattcaga cgaaatgaaa
     1741 tattgggaca gacgatatac ggcgacaaat atacaaaggt ttctaagtaa tgaaagcaaa
     1801 tttcaacaaa agcctacaac aagagcaaga atcgggtcgt ttggacgcca tgaactattt
     1861 atcattcagc ataaaacaaa ttttaaggag atttttatga ctaaattcaa aatcgttccc
     1921 ctgcctttaa acttagcaga acgcattcgc accgaccgaa tggacgatca aggtcacacc
     1981 gtcgtcgaac aaattgcgag cggctacggc ccgtgccgcg tttcgctgaa acccttcaag
     2041 cccggaacgg acagacgttt gctcttttcg cattctccgt ttgaagtaga taacgccttc
     2101 aaccagtccg gccctgtatt tattcatgcc gaatccgtaa cgccctatga agatatacac
     2161 cgatttcctc cggaaatcaa agccgataaa aagcactttc cattaaccct cattggttat
     2221 tccgatgatc agcgtatggt atacacccgt cttgtcggag atcgcgatgt agatcaaatg
     2281 atcagcgaca tttttgaaaa acatacagat gtcgcatacc tccatgcgcg caatgcggaa
     2341 gccggatgct acatttgccg gattgatcgt gcagaatagc tcaacaactt tggtatagtt
     2401 catacggtgc tttagcacga aaaatgcgac gggcggtaga gcccacacaa cacagctaat
     2461 cgcttgaatt tcatcaggaa tcccagtaag ttgatccagc attgatgaaa taagtcacga
     2521 cttgtttcgt tatgctaaga atcaaccaaa ggaaaacacc aacgatgaat atttcgtcgc
     2581 aaaccaattc ggaacaattc aaaaaagagt ttgaagaatt tttaaaagaa aaatacggta
     2641 cggccgatcc ggaacaagac agtgcaggca cgaaaaatca gggttcaacg ctgaaatccg
     2701 gtgaaaaaac caattttgac ctgaagcctg aggaattgga agaatacctc aaccaattcg
     2761 tcgaatgcca ggaagaagcg atcgaagtca tcgccaccaa aatcgccacg cactacaacc
     2821 gtatgaaact ggaagacacg ctcacggatg aacaaaaact tgtaggtaac atcaaaagca
     2881 atgttcttct catcggccct acgggtgtgg gtaaaacgta catcgtcaaa ctgatcgcaa
     2941 aaaaaatcgg tgtacctttc gtcaaaggcg atgccaccaa gttttccgaa accggctatg
     3001 tcgggggcga tgtcgaagat ctcgtgcgcg atcttgtacg tgaagccgac ggcgatatca
     3061 aaagcgccga gtacggtatt atctatcttg acgaaataga caaaatcgct tcggcacaaa
     3121 acaaccgcgg tattgatgta tcccgcaccg gcgttcagcg gaatcttttg aaactcatgg
     3181 aagaatccga agtggatctg cgtgtcccgc acgacatggc cgcccaaatg gaagccgcga
     3241 tggaagccca acgcaccggc aaagtgcaac gtaaaaaagt aaacacaaaa aacatcctat
     3301 tcgtcatgag cggcgctttc ggcggcatcg aagaaaacat ccgcaagcgc atcaatcaag
     3361 tacgcatcgg cttcggctcg gatcaagaag gcatctctaa actctcgcgc ggtgaactga
     3421 tgaaacgcgt aaaaacagaa gacctcgtcg aatacggatt cgaatcggaa tttgtaggcc
     3481 gcctgcccgt catcgccgta ctcaacgaac ttacggttga cgcactattt aatattctaa
     3541 aaaatcccaa cagcagcgtg atcctcagca aaaagcgcga ttttgccgct tacggcatcg
     3601 aagtcgaatt tgatgacgat gccttgcaca tttttgcaga acgtgcgttt attcaaaaaa
     3661 caggtgcccg cggcttagtc aatattttag aaaacgtatt gatcaagttt gaaaagaaat
     3721 taccgtccac gaatattcgt aaatttcgcg tcaccaaaga cgtcgttttg cagccgcaag
     3781 ccgaactcaa caagctgctc acctattctt cgatgaaaga atttcaacag cagtatctca
     3841 cgacgcacgg cattattttt gaattttccg atgtggccat tcaaaaaatc gctcaacgat
     3901 cacaaaccga aaaacgctct ttccgggcga tcgcggcaga gctctttaaa gattacgaat
     3961 acgggttaaa actggccggt aaaacccatt ttcttgtaac gcccgaagtt tttgatgatc
     4021 ccagaaaata tttggacgaa ctgataaaaa aatcgtattc caacaaatga gtcgtatatg
     4081 aaaaaggtag ttatcgttga cggcgtgcga acgccataca taaaagccgg aacacttttc
     4141 aataaaatgt cggcggttga tctcgggaaa ctggcgatgc gtgaactgat ggcgcgcacc
     4201 aatcttgaca gcaaactcgt ggacgaagtc atcgtcggca acatcggcaa tccgcctgaa
     4261 gccaccaatg tcgcccgcat catcgctctg gaggcgggag tccccaaaca cgtaccttcg
     4321 tttaccgtcg ggcgcaattg tgccagcggt atgcaatcta ttgccgatgc ctatctgcga
     4381 attatcgccg gtgaagccga tgtcatcatc gccggcgggg tagaatccat gactaaggtg
     4441 cctttgcttt tttcggaacg tgcaaccaat aaatttgccg cgatgatgca agcccgttct
     4501 atgccggcac gcatcggcgc catgacctcg tttcgccccg gtgatttcaa acccgtgata
     4561 ggacttctct tgggacttac cgacgggttt tgcggactta atatgggcga aaccgccgaa
     4621 gtattagcca aagaatacgg catcacacgc aaagagcagg acgaactggc actcctgagc
     4681 catcaacgcg ccacgacagc cacggacaac ggtattctta aagaagaaat catgccggtg
     4741 tatttaccac ctgattttaa aaaaatcgca gacgaagata acggcattcg aaaaaatcag
     4801 actatggaag cgctgggtaa acttcgcccg gcgtttgata aaaaatatgg caccgtaaca
     4861 gccggcaatt ccagccaaat caccgatggc gcggcgtttg tcctcgtcat gtcggaagaa
     4921 aaagccaaag cgttaggata tacaccgctc ggctccattc gcggtttcgg ctttgcaggc
     4981 cttgatccgg aacgcatggg actcggcccg gtatatgcca cacccgtcgc gctcaaaaaa
     5041 gccggcctct ccatgaagga tatacaacga attgagatga atgaagcttt cgccgcgcag
     5101 gtgatcgcca atgaacgttg ttttgcctct aaaaaatttg ccgaggaaaa attgggatta
     5161 accgaagcgg taggtgaaat tgatcgccaa atcctcaatg tcaatggcgg cgctattgcg
     5221 cttggtcacc ccgtaggttc atctgcaacg cggcttgttt tgaccgtact caaagagctt
     5281 aagcgaaaaa atcttcagtt cggtttagcc acgttatgca tcggcggcgg tcaaggcggc
     5341 gcgatgatcg tcgaacatag ttaggcaacg tattgtatgt acggattgac cactgaagaa
     5401 ttagcacgat acaatcgtca tctgattctc aaagaatttg gcctcgaagg gcaacaaaaa
     5461 ctcaaatcag ccaaggtatt ggtcatcggt gcagggggcc ttggcgcacc atgtttgctt
     5521 tatcttacag ccgccggcgt aggaactatc ggcatagttg actttgacac cgttgatgca
     5581 agtaacctcc aaaggcaagt cctatatact gtggatgatg tcggtaaacc caaagccgat
     5641 accgccgcgc accgtttgaa gcagctcaac ccgtctgtac attttaatac ccatcgcgaa
     5701 aaactcactt cagctaacgc gttggatatc atacgatctt acgacatcat cgccgacgga
     5761 agcgataact ttcaaacacg gtatttggtc aatgacgcct gtgtgttact caataaaccg
     5821 ctgatctacg catcaatatt tcaattcgaa ggccaggttt cggtttttaa ttatacagat
     5881 ccatccggta cactcggtcc aaactatcgc gaccttttcc ccaccccgcc gccgcccggt
     5941 atggttccca gttgcgctga aggcggtgtc ctcggagttt tgccgggcat tatcggcagc
     6001 atgcaagcaa gcgaagccat caaagtcatc accggtatcg gaacgccgct tagcggcaaa
     6061 ctttttctgt ggaatgcgct gacgtttgaa tcacgaacta tgaaatttaa gcgcgatccg
     6121 gccaatccga tcaatggcct gcaacagacc atcaccgcat tgatagacta tgaagttttt
     6181 tgcggaattt ccgaacccga aaaacaaact ggcggagaaa tttcaccggc agaactcaaa
     6241 cattggatag ataccggaaa agattttcaa ctcattgatg tacgcgagtc atttgaatac
     6301 ggcatttgca atctcggcgg cacattaata cctcttgcgc agattatgca agaatcggcc
     6361 cgcatcgcac aaaacaaacc ggtcgtggtt cattgcaaaa tgggcggacg cagcgccaag
     6421 gccatcgcgc aactgcgcac gctgggcttt gataatctca tcaatcttcg cggcggtatc
     6481 atcgcgtacg ccgaaaccgt agatccgtca ttggctaagt attgacgtat tagcgcacat
     6541 cgtagcgcag aaatcgcacc aacgctccca aaaaaacaaa tcctgttatc acggcgagga
     6601 gtacaatatc ccaaaccgat aatcgccatg catgcccggc ggagacggcc ggatgtgtaa
     6661 attccggaat ggatcgcaca tcaatcgttt ttttcaaatc cggtgtttgc acacgaaacc
     6721 cgcgatcgct atccacctcg atacgcagtc cttcggccct attttctcga cgcttttttt
     6781 ccacaacatc ggaaaaatct ttttgatagg tacgtatcgc gtcgtcgtaa cgttctttca
     6841 gaaaaatatc ggtctgtgaa aaacgcattg ccgcgagttg aaataacgaa gtcggcgacc
     6901 agcgcgcaaa aatcaaaccc agtttgattt gctcgcgttt tcggttatcc atatcttcat
     6961 gtactttgcg tgtaaaactt tcaatgtttt cctgtacgga cttgcgggca ttttgatctt
     7021 cttccagcca tgtccactcc atcttatcgc ggtacgtctg tcgctcggat tccggctgtt
     7081 gttgcacgtc attattgcgt ttcatccagg tttccgagag gttttggcgc aatgtttcaa
     7141 attgtgactt agaaaagcct tcgatttggc tatccacttc ggcggctgtg ggcaccggta
     7201 aaacctgccc cgatagcata acaccgaggc gcggcacgac aaaggtgatc gtaacccata
     7261 atacaagtaa agtgagaaac gatgcgttcg agtgcgaagt ccatgatgaa atcagaattc
     7321 ccaaaataat aaaaaaagta aagtatagcg cacccgccaa aattaaaccg cctaaacgag
     7381 catagtgatc gggtgtgaga ggtatttgaa atagtatcgt catcaaaaat gataacagca
     7441 tcggcacgat caaaggaatc cccaaccccg cccatgatcc gataattttt gcaacgatgt
     7501 atgtggattt agggacacca ttagaaaacg taagcttcaa cattccgctt tctttttcgc
     7561 cgcttaccgc atcgtacgta aaaacgatag caaataacga taacaccacg acaactataa
     7621 aattaaaatc caaaaaccga aatacggcat atacgagatc gcgggaataa atgctgctgc
     7681 gcagttcggg tgaatcccat gtattgacat gggcgacgcg tccgatatcg taatgaactc
     7741 cgaaggtcca tatctgcata ggatccggtt tgcgatatac ccgattacct acattcatcc
     7801 aattggttcg ttcactcaaa tcctgccggt taagcgctat gccggtttcg taatctttca
     7861 cggccagctt gtattctcgg attccgaccc acacactgag aatgatcaat agcgtacacg
     7921 atataaatgt ggctataaac ttaggacttt gcagtatgtt tttaaattct ttgatgatca
     7981 ataacgataa catgggtttc tccttttacc gtacgtcgta acgaaggaat gcagccgtag
     8041 tagccgcaaa tatgagcata ttgatgacga tcaatatggc catatccggc aaagcggcat
     8101 tgattatttc tgaaaaagtc agcgatggca tcacaaattg cggtaagtcg taaggattga
     8161 taggtttcgg tttttcttca ggcgaatcgc cgatacgaat catcatcata ttaccaccgg
     8221 gcgcttttcc cgttttttca aaaacaaatt gtttgaatgt ctgtttatac gatttgacgg
     8281 actcttcaaa acgattacga agttccgaag atgtccccgc caattgcatg gcggcgagcg
     8341 aaaacgacgt cgccggtgaa atacgtccca atgcaaaagc ccattgctgt tgtatttttt
     8401 gccggttatc gcggtcttca tttaatcgtt cattaaattc gacgaccttc ttttcccgat
     8461 cagcgcgcgc tttgtccata aattcgttaa attcattcat tgctttttca ggatcgtttg
     8521 aatttggttt aaattcgccg attttttgaa aatgttcatt aaagagctgc gattgatgac
     8581 gaaacttttg tgagttgatt tcatccacgg acggcacttc gatcgcccga cccgctaaga
     8641 gcacggccgt acgcggtacg atcagcacgg tgaaaatcca gataatcaaa agcgcaagaa
     8701 atgcatgggc cgatcgggcc gtcagcgagg aaaccaagat agaaacacta agaaaaacga
     8761 aataatacaa catccctgaa catacgatca gcatcaggcg cagccactcc gctccggtca
     8821 gcggtacatt catcaccatc aatataccgc agccgatcaa tataggaacc agcaaaggca
     8881 gagccgttgc ggtaaaactg ccgacaagtt tagcaaaaat cactttatct ttgggcacag
     8941 catgcgccat catcagtttg agcgtaccct gctcgcgttc gccgttgaca gcattgtaag
     9001 ccagcaatat cgccactaac gataaaatta actgaaatac aaattccaaa tccagtaacc
     9061 ggaatgccgc gtaaacggga tcttcaccat aacgcgtatg atcaggaacc aattcggcgg
     9121 caccggcctg cacttgtact gagcgaccga tatcgttgct cacaccggat ataagactgg
     9181 ctaacggcga aggtggcaga tgtatttgtg gttcgacgcg cgaccaatct gtcacgcctt
     9241 ccaattggcg taaattttct cgcaccgcgc tttcgtattg gttttgcaga acttgataat
     9301 tacgcgcacc catataaaac gccaacaata tgagcaacga tcccatcccg aaaagcgcgg
     9361 aaaacttggg tgagtttaaa atatcgcgta tttctttttt caacagtaac gtaaacatat
     9421 agttttatat ttaatttgtg aaactaatcg cctaattcga tctgtaaagc ttcaaaacgc
     9481 caaggcgctt ttgtttcgat ttgaaaatta aaaattgctt tagctttcgc cgagcccaca
     9541 tgtacttccc aagaaccatc cgcacgtgtt tgaaatgaat tcacggttat aacacctaaa
     9601 tccgaaaaca aacgattata attttgcaaa cgccgttcca tcggagcaaa accgtctccg
     9661 cttttggcat aattttcaaa aaacgctttc atcgtattcg aattgccgct attgaaggcc
     9721 tcaacatatc gccggatcgt caatcccgca ccctctttgg gtaaatcaaa aacctttgca
     9781 ctctcgccgg ggttatctac ttcaacggtt ttgataactt tccccatagg atttttaccg
     9841 ttgcgtaaat acccctcaat ggattctaac aattgatgcc ccatgtagtt atcatgatta
     9901 ctggcaatca tgagccgcgc tttgatacct ggaaaattgg caaacccata gaacgaaatc
     9961 atatctgatc cggccaacac cgtaggttgt ttggagtcat aaacggccat gcgaaatgcc
    10021 ggcgtactca atagtttgcc cgtttcaaat gaatcaaaaa aaagtttcat atccctaaac
    10081 gtagataaat aaccaccgtt ccctcgtaaa ctccatagat gcccgtcttg atcatgcggc
    10141 atatccaaaa taataccgat atccgcattt tgacggtatc catgggctac cttattgcga
    10201 tcaaaattcg ggcggtatgt gccggtatga tgcatgccta aaggcttcag aatgtactca
    10261 tccacatagg catcaaatga acgcccgctt actttttcaa tgacataagc aaggcatgaa
    10321 taacctagat tagaatattt ttcgtgcact cccggacttg agagtaaatt taaacctctg
    10381 atatgttcga ccatttcttc tttcgtcagc ggctccgcat caaatttgat gccgatagga
    10441 aacccggcgg tatgattcaa taagtgctga acggtaatct tattcttttc ccctgcaagg
    10501 tcggatatat atttagacag cggatcgtcg agagataaca attttttttc gattagttga
    10561 taaatcgcaa cacgcgtaaa atctttgacc gtagaaccga tttgtacaaa aaccgtactg
    10621 tcaaattttt ggttattact ttcgttagca aaaccaaaac ctttgtagta gatcggtttt
    10681 ccgttgcttt caatccatac cagtccatga aacccgtctc gtacagccga gtgtatcaat
    10741 gaatctaaac cacttacttg tacatcacgt gattgagcta cagtaagtga actaatgaac
    10801 cataccatga aaaataattt tatcataacc accttactaa tgtttagttc tatacggcta
    10861 ccggttccat ataacccgcc atatattcaa tgtagagttt ctccaaatct tcatgagcca
    10921 actcatcgcg gtttttctgc atcactattg agccgcggtt cataattccg atatgatcag
    10981 ccacttcctt tgctctgaaa atatcatgag tggacatcag gatcgcttta ccctcttgtt
    11041 ttagttcgat cagtaatcgg ttaaattcat atcctgcctt gggatccaaa cccgatgtag
    11101 gttcatccaa aataatggcc ggagcttttt tgataatagc gatggctata ccgcattttt
    11161 gccgcatgcc tttggaaaaa gttcggactt ttttgttcat cgcttcgctt tgtagcccga
    11221 cgcgcgataa cgtgactttc atttccgatt cggttatatc tgcgcgtccg cccagctttg
    11281 caaaaaactc aagattctcc atcgctgtaa aattaggata gagcatcaca ttctccgaca
    11341 caaaagccat ttgttctttg gcccttaaag gttcctgatg cgtgatgatt cctttgactt
    11401 tggcctcgcc ggatgtcggg gtgatgaaat tgaaaaaaat attaatcgtt gtcgttttac
    11461 cggcgccgtt ccctccaagc atcgcataga tttcaccggg atgaacctga aacgaaatac
    11521 cgttaagcgc taaagcgcca tcgtcataac gtttggtcaa ttgtgccgct tctaaaacgg
    11581 gttgattgtg tactgtcata aatacctcta tttacgtgtg agtttaatgc caaaccaaac
    11641 tattccaccg ataatgctta gaatcagaac gatcagcgcc atcgtgccca aagcattact
    11701 tcccgcctga atatcaatag tgattacttt atcttcggcg ttgaccggct gattatcaga
    11761 gacggatgat gttcggatgc gcacttcgta tttaccgacg gccacgtgtt ccggcggata
    11821 aaacttgaat cgcacaaccg actcttcgtt tacatggatc gagggaatca tcagcggttg
    11881 tatttccttt ttccattcca aaggcagatc cacttcaaac ttgatgttat ccagacgacg
    11941 tgaaccgtcg ttgcgaattt caagcgatgc ttctacaggc tcggattctg tcgtggcata
    12001 atacaacttc ggcgctttga ccaataaacg tgcattgcct ttgacgatga gttcaagtcg
    12061 gagatagcct gcgttgagcg attcgatctc ttgttgggac cacaccttat cgcgttgtgt
    12121 ttggatgagt gcctgctttt cctgcggtat cgctaataca taaaacggaa tagttttttc
    12181 gatttggact tgttcggtag gacgatccgg caaaaaaacg cggagcgatg ccttacgggt
    12241 acgcgccgat tcggcaaact ttaactgact gagtttagca ttggttgccg catcaagaaa
    12301 atagcgcgtc agttccggcg ggagattcaa tacttccagc ttaaacgtat tgtccgtgcc
    12361 gctgaataac tccaacgtaa gatcaaatgt ggcattactc ccgagatccg cttcctgtga
    12421 aaactgctcg gattggaata atactttatt ttcagtagcg tctttttgta agaaaatccg
    12481 catgcttcgc gatgccccgt taccgtaaat aatattgacg ctcaccacat ccaaatcttg
    12541 taacattaca aaatccaatg tcacgggctg tccgtagacc aattcgttga ctttggattc
    12601 gtacggttgg ctgatgatcg caccttccgt gttcgttacg gaaacgtaga tattatttat
    12661 gtgatccggt tgtaaggagc ggaataactc atcttccatg gaaatcaact tacgaaattc
    12721 agcttcaccg ccggaagtgt tcatcagttc gacgcgaaca cgcttctgtc cgcgtttgtc
    12781 ctgatattta accgcgcgtg acaccgtgat aaattgctgt tcaaacagca ccgccaacag
    12841 cgattgctga tagttcacct ctacgtcggc cagtgaacgt cgggcccgtt ccagttccgc
    12901 cggcgaagcc agttgtttat caaacaattg tttcacgcgc tcgtactcgg ctttttccga
    12961 ttcataaacc tctttggcgc gttttaatcc caacagcgga tatttatcat cacgctggtt
    13021 aaatgcatta tactgcgcca tcaagccgga atcggtgaag caaaagaata gaatcgtaaa
    13081 ataaaaccac cttaacatta tttggtttcc tctatttctt ttccgtccgg ggtgcgtttg
    13141 atcattttac gttttggtaa atgctcggga tcgattttgg caaaactggc aatcattttt
    13201 tcatccccgc gtttaagccc gagtttgatc gtatcaccga ccttgacttt atcgtaagcc
    13261 gctttcaact cgtttgcggt ttttaccgaa accccgttaa ccatcaccaa cacatcattt
    13321 ttttcaatcc cttgaggttt ataacctttg ggtaatgcgt gttcaggaaa aatcaaatcg
    13381 gcaatcaaac cgttctcttt ttcagcaaat ataaccccga cttcatccac cggctgcaca
    13441 ggacccgtag ccatatagcg aataccttgc gcgaatgcat ttcctccgac accgaacaag
    13501 gtcataccta agatgagaat tatttttttc atactgcgtt ccttccaggt tttatagacg
    13561 atgatgaatt acgctaatga tacttgattt tagaactaga gttgttaagc cggcgtaaaa
    13621 gagttgtaaa ctatgtaaaa tatgaaacgc cgcacacatt tcgattgaag accgctgttc
    13681 gaatgcctat ttttcactat ggcaaaatca aaacgattac accccggatg gattatcgcg
    13741 ctgcttacca tagcgctgat cagtattggt acaatgcaat atatttggct ggggcgactg
    13801 agcgaaggcg aattgacgcg catgcggagt aatctccata tcagcgctac gcgtatggca
    13861 tcggattttg acagcgaaat atcgcgcctg cacgaatatc tttttcgggt ggactttgat
    13921 caacctgaac agttacccaa tattcttcaa actaccgtca ataaatggca aaccaatgcc
    13981 gcacgccagg atttgatccg ttccgttttt tacggtcgta tgtctgaaaa taacacgcct
    14041 gaggtttttt tgttggattc ggcaagaccc gaacttcatc gggtttccga cacagtgtca
    14101 aaccccatcg ttctcaaatt acaacggatg cgcgctatgc gctcggtcgg taaggaaaaa
    14161 aacaatctga ttatcatgca aggccacctt cttgccgact acccatatat catcgtgacc
    14221 gtcaatccga tgcgcaaaat gctaaacctg ccgtgggagc aaccggctga ggaaatcatt
    14281 gctgttcttt tggatacgaa tgcggtcaaa tcatggatgg atagtttagc aggtattcac
    14341 tttggtgcat cgttcgaaaa tgatgtagat atcgctgtga tcaatgatga ccaatctaaa
    14401 tttttttaca aatccaattc taatttgccg atcgcacact ggtcaaaatc ggatatcagc
    14461 gctgagatcg cccgtattcg cacccaagat atgtttatgg ttcgcgtcaa aggcacccgt
    14521 gataaaacaa ctatgaaaac cttgaccgta acgcaatcca ttgaaacaag cgaacgtacg
    14581 tcaaacggcg catttaatcc gatcattgat tcgatcgtaa cgcagttgca tgccgacacc
    14641 aagacgtcag gttgcaattt acgggtcata catcgcgaag gctcattgga agatgccgtc
    14701 aaaatcgtac ggtggcgaaa cctttttctt agcgcaggcg ttctgcttat catggcctca
    14761 gggctcgtta ttcttttgat cgcacagcga aaggaaagaa tattagcaaa tcagcaattg
    14821 gaatttgttg ccggagtttc acatgaactt aaaacgccgc ttgccgttat tcgttcagcc
    14881 ggtgaaaatc tttccgatgc gatcgtcacc gatgccgatg atgtgcggcg ttatggcgag
    14941 ttgatacgtg gtgaaagcaa acggctatcg gacatggtag atcagatttt ggatttttcg
    15001 ggtatcatca ccgataaaaa ctcattaaaa atggagcgtt tggatttatc cataattatc
    15061 caaaaatgca tcgcctcatt ccccaatgac accccgattt atttcgaaga ccgccaaacg
    15121 acacataaca tgattatcgg aaatcatgaa gctttgcaaa cgatatttaa caatctgatc
    15181 gccaatggga tcaagtacaa tcgcgccaat gcaccgatac gtatcacgtt gaccgatgaa
    15241 tcgccgtata ttcgcaccga tataaccgac tgtggtattg gcattgataa aaaagatata
    15301 cctcacattt ttgaaccgtt tttcagagcg tccggtgtac gcgatgcaca gattcacggc
    15361 aatggcctcg ggctcagcat cgtggaacgt tatgtcgcat tgcatagcgg cacgatccgt
    15421 gttgagagcg aacccggtaa aggaagtacg tttagtgttt ttcttccgga gggaacgtga
    15481 aaaaattatt actggtcgag gacgaacccg gtttaattat aactttgtcg gatcgcttac
    15541 ggcgtgaagg attcgtcgtg gacatcgctg aaaacggcac catcgctttg agcaaagccc
    15601 gaaagaatcg ttatgatatt attttgctgg atgtgatgct gccgcaaagt tcaggcttgg
    15661 acgtgtgccg cgatttgcgc ggtgacggcg atcgtacgcc tatattgatg ttgaccgcca
    15721 aaagccagac gaccgataaa gtgatcggac tcaagctcgg cgccgatgat tatctgacga
    15781 aaccttttga tatgagcgag ctcacggcac gtatcgaagc gctattgcgt cgcagtgagt
    15841 cggccaatcg catggaggcg tcggataatt acagtttcaa caatattgag gtggattttc
    15901 gaaaagccga agtacgcgtc cataaaaaaa cggtggagct ctccgctcag gaatttcgat
    15961 tgctgcgcta tatgatcgag catcgcaatg cgactttgtc ccgggatgaa ttgcttgaga
    16021 acgtttggaa ttatggcgaa gacatcacaa cgcgcacggt ggatgtgcac atggcatggt
    16081 tacgtcaaaa attggaaccc aatcccaaac ggcctcagta ttttatcacc gtccaccgtc
    16141 taggttacaa atttgtcggc tgactcgcct ttttttctaa ttcagcaatc gcttcgcgca
    16201 tccacgacgg cattgggcat ttctgatttt gattataatc atacacgacg atcaatcctt
    16261 cgccttcggc cacgatggcc tgttgcgctt cactccatac ggcatatttc atgcgaaaac
    16321 gatcggccat cacctcgacc gtcgtagcgc ccacgtgaat ttgatccgga taggtcagag
    16381 gtcgtttaaa gcggcactgc gtcgaatgta gaataacacc gacaccgtgt tcccgtgcat
    16441 gatcccatac accgatgcgt tgaaaataat cggagcgcgc actttcaaaa taacgaaagt
    16501 acatgatgtt attgacatgc cccagcgcat ccatttcgcc ccatgccacc ggcgttgaat
    16561 aaaccaccgg atagccgctc aattcagcaa tccctttcat catcaactac gcaaccctca
    16621 cgttgttcaa acgttttatg caaatgagtc acatgcaatc gtctaatcgc aaatctaaaa
    16681 ttctaaatta taaactcata actttgcatt cataactctt atttttttct caaaactcgg
    16741 ctttctttct tcccttattc tgttggagca gccagtcata aagcgggccg taataatcca
    16801 acatggcttt tgcagacaag tcctgacctg ttttttcttt caaaacttga cgccaatctt
    16861 tgctcgcgcc cggacgcata atcgagcgaa taaactcacc ggcttctttg cttccgtaat
    16921 aattgcaatt ggccggatct tgcttgagta tctgcttggc aatatggtca tgcatttgga
    16981 atttcaaaac acacgaaatg gcgtaatcat aatactgcgc cggatcgtca atgatgtgcg
    17041 ttttggtagc cgcatcacaa tattcggcac cacgctcttt cggtggaact ataccttgat
    17101 atttttttgc cagtacccac catgtcttat taaattcttc tttgggaaga tttttctcgt
    17161 acagttcgta ttcaaaatgt gtcatcgtac ccgcagcaaa tggcagaaaa acgatcgaac
    17221 tattggataa agcgctgttg agcaaaagtt tgatcgcatc gggcgtctta gttttatcaa
    17281 ataaacctaa actggctaaa taagcctctt gcgatgcagc cacagccatc aggtcaccca
    17341 tcgcttcatg gtacgcacga ttagctccgg cacgcaaggt aagcggcact tcaggattgg
    17401 tataagcgat gtagtaataa atatgcccga gctcatgatg cgtcgtttta aaccattcca
    17461 tattgggttc gacactcatc agcgaacggt aatcctgatc cagattcagg tgccatgccg
    17521 acgcatgggt attctttttt cgtccgcttg cggcatcggc aggatacaaa tcagaatttt
    17581 tataaaaatt atcgttcagc ttttcaaacc ccatgctcat gtaaaatttc tcagcttgct
    17641 caacgaccca ttctttggat tttccgggta tatccccttc accctgcaat cccggaacta
    17701 atcccggcca attttgtgcc caacgattgg gcagccagtg cgccggaaga tactccggaa
    17761 cgggttgatt atactttttg gctaattcgt agcgtatata cgtatgcagt tctctataca
    17821 gaggtttcag atcttctgaa aattgatcca tcagttggat cacttcgtcc gatgacattc
    17881 cataatcgtg cgcttccaga tcaaaaaatg aactgtactt catttcgcgt gctactttat
    17941 tccgcagctc ctgcagtcgg gcaagatggt cacgttcgat ttttccgact tctttggacg
    18001 cggtccaaac cgcctcgcgt tccttgagat cggaagaatt gcgcaacgca cgatcaatat
    18061 cattcgctga ataaggtttg ccgttaaccg taaactggta gctatacaat gcactggtcg
    18121 cttccgtatt ggctttgatc agttgatcca ctacatcagg catggtaccg ggttgatgcg
    18181 acgcgtccag ccatatttta tcaagctgac gtatcgttat gtcattgagt tccgaacggt
    18241 gttctctgaa aaaccgtact ttctgaatga tgtctttgct gcctgcaaac gcagcatatt
    18301 ttttattggc taaaacactc aaactgtcgt ttaaggcatt gatgtccgtg agtgctttcc
    18361 attgggcttt gctggcttca taataaaccg cctgatactc ttgattataa gatttcaaaa
    18421 aagcatccgc ttcttcctgc aacgtcttac cgcaagacaa cagcgaagta aacaaaaaag
    18481 cagttataaa aaatcgtttc atgatattct ccggtaatcg ttataaatca taactgaatc
    18541 ttcgaaatgt agaaattcaa tggaaaataa caaaccaaaa caatattttc ctccgttgta
    18601 ttttagatgt agaagaaagg ataatctatg aagcgtgtct taaaatggtt ggctatcggt
    18661 ttaccggttc tttttatcgc catacagttt attcgccccg aacgtaccaa tcctcccatt
    18721 gatccggaac gtacgctcga aaaacatgtc accattccgg acgatatcaa aaccatgata
    18781 aagaccgctt gcaacgattg ccattcgcat gaaaccgttt ggccgtggta cagcgaaata
    18841 gcgccgattt catggctggt cgcacacgat gtcaatgaag ggcgccgcca tttgaatttt
    18901 tccgactgga aaaaatacaa cgcccatcgt caggttgaaa aattaaatga aatggccggc
    18961 gaggtgcgta gcgaaaagat gccgatgccg atttatctga tcactcacgg ggacgcccgc
    19021 tggacgaaag aagatcggaa aaaatttcaa aactgggccg aagatgccgc ggacgctttg
    19081 tcggaaaatt taccggacga agacaaataa ttcgtttgct tttataaagc atttcgatta
    19141 tagtttcaaa aaaaattgaa actttgttag cgataaaata aggagtctac aatgtctgtt
    19201 gccgaaaaat tgaatgtgcc ggcctacgtt ccttacaaag ttaaggatat gagtcttgcc
    19261 gagtggggcc gcaaagaaat caaactcgcc gaagccgaaa tgccgggact tatggcgttg
    19321 cgtgccaaat acggcgttac caagccgctc aaaggcgcgc gtatcgccgg ctgtcttcac
    19381 atgacgattc aaaccgccgt tttgatcgaa accttggtcg aactcggcgc tgacgtcacc
    19441 tggtcatcct gtaatatttt ttcaacacaa gatcatgccg ccgccgccat agccgccgca
    19501 ggtattcctg tctacgcctg gaaaggtatg aatgccgaag aatttgactg gtgtattgaa
    19561 caaacattgt ttttcggtaa agatcgccaa ccactcaata tgatccttga tgacggcggt
    19621 gatcttacca atatggtcct ggataaatat ccgtcattgg ccaaaggcgt acgcggtatc
    19681 tccgaagaaa ccacaaccgg cgttcatcgt ctttatgagc gcgtcaaaaa aggcacgctt
    19741 ccccttccgg cgatcaacat caacgattcc gttaccaaat caaaatttga taacaaatac
    19801 ggttgccgtg aatcgcttgt ggatgccatt cgccgcgcta ccgatctgat gttagccggt
    19861 aaagtcgctg tcgttgccgg ttttggcgat gtgggcaaag gctcggccga atccctccgc
    19921 gatgccaagt gccgtgtgat cgttacggaa atcgatccga tctgcgcact gcaagcggcg
    19981 atggaaggtt atgaagtcaa aaaaatggat acagccatca aagaagccga tattatcgtt
    20041 accgcaaccg gcaactgccg catcgtgact gatcgccatt tccgcgctat gaaagataaa
    20101 gccgtgcttt gcaatatcgg ccacttcgac acggaaattg atatggcttg gctcaataag
    20161 aactatggcc acaccatgga cacgatcaaa ccgcaggttg ataaatatac cgtggacggc
    20221 aaagagatca tcgttcttgc cgaaggacgt ctggtaaacc tcggctgcgc tatgggtcac
    20281 ccttcgtttg tgatgtctaa ttcgtttacc aatcaaacgc tggcgcaaat cgaattgtgg
    20341 aataacagtg cgaagtacga aaacaaagtg tatactttgc ccaaacatct ggacgaaatg
    20401 gtagcacgtt tgcatctcga aaaaatcggc gttgagctgg acgaattgac cagcgaacag
    20461 gccgaataca tcggcgtgga cgtcaaaggg ccgtacaaac ctgagatgta tcgttattaa
    20521 taattacgca cgactctgat gatcattgag cgatcctaag agtttaggat cgcttttttt
    20581 tatattatcc caaaggttgc ttgtgaaatc attcaccgaa ctgctgaacg aaaaaattat
    20641 cgtatttgac ggcgccaccg gcaccaatct gcaaacccag aatctcacgg ccgatgattt
    20701 tggcggcgaa gcttataacg gatgcaatga atatctgctc atttccagac cttccgctat
    20761 ggaaaaagta cacgtggatt ttcttgaggc cggtgccgat gtcatcgaaa cagatacgtt
    20821 tggttcctca tccattgttt tggccgaata cgatcttcag gatcgcgctt acgaattgag
    20881 caagttgggc gccgcgttag ctaaaaaatg tgcgctggat ttttctactc ccgacaaacc
    20941 gcgtttcgtg gccggttccg tagggccgac gaccaaacta ccctcgctca gtcatattga
    21001 ttttgaaacc atgcgcgcat cgttttatga acagttggcc ggcttaatgg atggcggcgc
    21061 cgatctgttt tgcattgaaa cctgtcagga tatcctacaa accaagatcg ccctgtgcgc
    21121 tgcacaagac ctgttccgtg agcgaaaaag aaaactaccc gtcattgttt ccgttacggt
    21181 agaaactacg ggcacgatgc ttatgggaac cgaaatcagc gcggccttga ctacgctaga
    21241 gccttatgac atcgtcgatg tcgtgggtat gaattgtgca accgggcctc aggaaatgtc
    21301 cgacaatatt cgttttctgt gtcataattc acccaaacat gttttttgta tgcccaatgc
    21361 cggcataccg gaaaacatcg gcggtaaagc gcattatcac ctatcgccgg atgatttagt
    21421 caaatggttg atgcattttt ctaaagattt cggtgtcaac gtcgtgggtg gttgttgcgg
    21481 tactacaccg gcgcatatca aacctctcgc cgaagcggct gcacgcctca atccattaaa
    21541 gcgtacatgg aattatacgc cgtccgttgc ttccatttat atgagttcgc cgatgcatgt
    21601 cgaacccgcg cctttactca tcggagagcg cacaaatgcc aatggctcca aaaaattccg
    21661 tgagctcctg atcgccgaga attacgatga aatggtttcc atgggaaaag aacagacccg
    21721 cgaaggcgct cacgtgcttg atgtgtgcgt ggcgtatgtg ggtcgcaacg aagtgcgtga
    21781 tatggaggaa accattcggc gcttcaatac ccagatttca attcccatta tgattgactc
    21841 gaccgaagtc cctgtgattg agcgcgcctt gcaaatgctc ggaggtcggg ccatcgtcaa
    21901 ctcgatcaat ctcgaagacg gcgaagaacg tacggacaag gttttgagtc tgtgcaaacg
    21961 ctatggagcg gccgtcgtcg cgttgacaat agacgaagat ggtatggcca aaacgcgtga
    22021 acgtaagtta gccatagccc gacgcattcg aaccatagcc gtcgaaaaat acggcatgcg
    22081 ggatgaagat cttattttcg acacactgac ttttacgctg ggctccggtg atgaagaatt
    22141 tcgtaaagcc ggaattgaaa cgatcgaggc gatccgtttg atcaaacaaa cctaccccgg
    22201 ctgtaaaacc ttactcggcg tgagcaatat ttctttcggt ctttcgccgc aggcgcgcca
    22261 tgtgatcaat tccgtattcc ttcactacgc tatcgaagca ggcctggata tggctatcgt
    22321 caatgcgcaa aaaattatgc ccttgtacaa gattgatccg catgaacgtg aacttgcgcg
    22381 ccaactcata tttgatgaac gcaaatggga gcccgccgca tgatcgtccc tatttttcgc
    22441 gtattgtcta ttctttgcgg attattcttt ttgatgaccg gatggatatg gcaatatctg
    22501 atggctttat ttgttttgcc ggtcggtatt gctggaatga ttttatggtt ttcggctaac
    22561 cggatggcga agaaattacc ggatgaaaac gaaaccaccg cacgttggtt gaaccaagcc
    22621 gcgtggtgga tcaatataac ggcttttcta tcgtccggtg tggctttgct gtataatctg
    22681 gttgccgttc gttaattcgt aactttttcc cacaaccaga atttgtcgat ttcccccagg
    22741 agactggtgc gcgaattttt tattttttga ttatacccta ctaattcatc acgccaatat
    22801 aaaaaagtcg ccggctgatc ttcgtgcagg atcgcctgca aagcacgcca catcggcgct
    22861 gcatcttcgg gattgtttac gcgcttgccg gcctcaatca aagcatctac gttgggattt
    22921 tgatatgaaa tatcgttata cggatttttt ttcaaatccg aactccactc gcttgtcgga
    22981 tcaatcataa gcggcacgcc gataccggag ataaaggcgt cgtatttttt attcaactcg
    23041 tcatcataaa acacatttgt ttcggcggtt cgtacgtcgc atcggatgcc gatttttttt
    23101 aggtcacgct gtacaataag cgccgcaaat tggcgccggt cgttgcccgc atcgtagtat
    23161 agttcaaatg taaaagctct gccgtttttt tctacgatgc cgtcgccgtc gcgatcaatc
    23221 caaccttctt gcgctaaaag acgcttcgat tcttcgggtt cataaggcag cggactcaac
    23281 gtatcattgg ccgcccaacg aaagaccggt gaaatcggcg tgatcgccac acgcccaaat
    23341 tcccccataa acccatctac gatttcctga cggtgaatcg ccatcgtcaa tgcgcggcgt
    23401 acgtgttttg aaccaaacaa cggatggggt tgaatagtat taccggacga tgcatgatat
    23461 gcatgctgat ctatattctg ccatccgaca taatcataat aacgcgaacg catcgcctcg
    23521 atacgcagtt ccggattggt tttgcgtaaa tccgccacgt cttgcggcat aatcggatac
    23581 atcacatcca catcacccgt tttaagagcg gtcaaccgcg tggtatattc gggaataatg
    23641 cgaaacacga tttgacggga agcggcctga tacggactac gcgcgttgcg ttccagtacg
    23701 atataatctt gttttttcca gatggttata tcgtaataac ttccggataa cggcatggtg
    23761 ttgatagtgt gccgccgcaa ctcggagggt ttaacatctt ttaaaatgtg aaagggcaaa
    23821 aatccgggct gaagattggt atcaaaaatc tgtagcggat aggcacgagt aaaatggaat
    23881 tcgatcgtac tatcattgag cgcgatgacc gatcggtcca cggatcccag ggcatccgta
    23941 agatactgta tatgttcttt ttttgtgctg gctacatccg gatgggcgat catttgaaac
    24001 gtaaagcgaa tgtcatgcgc cgtcacacgt tcgccgtcct cccaaaaaaa atcggatcgt
    24061 aagtggtaag tcaaaacgcg gcgatcgtcg gagaaagaat acgatttagc gagaaacggt
    24121 ttgtacacgg cacgaccttg cagtgaatcc cattccgact ccaacaaacg cggcaaaata
    24181 ccggtacaaa caattctgga aaaagccgaa gtgtgtgtca cgggattgat tgcatccggc
    24241 tcggtgcgca cagcgacagt tatacgctcc tcttgctgcg ctggaacaca actcatgaga
    24301 aagctcgata tgaaacaaac cgctatggct ttaatcatca tacaccggca atatgaagca
    24361 tatcttcaat gcgtgtaaat ttgatgcgcg gtcttccgga ggccatgccc ttttctgttt
    24421 cggctttatc gatttttaac caatcggcaa atgttacaaa acgaattttc ttcgattgaa
    24481 gccagtctgt ggtagatgca gcttgatcaa tgggatgaat atgaccggac ttcacatcct
    24541 ccaaaagcat attcaccgat tccagcgcgt ccggcttatt agtaccgatc actccggatg
    24601 ggccgcgttt gatccagccc gccgtatatt cacccggaat gacggtacca tttaattcgg
    24661 ttacccggcc atgtgcattc ggtatgatac cttttttttc gtcaaaagga acatctttga
    24721 gcgcaacgcc tttataaccg atcgaacgaa ataccaaacc gacgggaatc gtttctttag
    24781 cttcagttcc cttcgctttg atgctgccgt cggcatcttg cactaagatg tttttttgta
    24841 aaacgatctc ttcgacacct tcccgaccct taagttcgac aggtgaccat aaaaaccgaa
    24901 gcaccatttt tttcctttta ccgtcggtcg ttttatccag ataatccttc ataatttcaa
    24961 gatttttgga aacaccggaa tccggatgcg cctccaagtt ttttttggat acttcgtcca
    25021 aagcgccctc cgtacctgga atagtgatcg cacattcttc caattccccc agctctttga
    25081 tctccgcatt tgtaaatgcg gcttgcgccg gaccgcggcg accgatcatg tatatttctt
    25141 ttacattgct ttttttcaag gcgaccagtg catgatccgc gatatcggtt ctgttcagtt
    25201 cttcaatact gctggctaaa atacgcgcca catccaccgc gacatttccg tttccgataa
    25261 tagcaacgcg ctcttgagaa agatcaaatg acagatcacg ataatccgga tggccattat
    25321 accaggctac aaagtctgta gccggatggc tgccgttcaa gtcttccccg ggaatgccca
    25381 tgcggcgatc actttgtgca cctacggcat aaatcaaagc atgataacgc gtcttcaatt
    25441 cctgacgcgt tacatcgcgt cccaactcca catttccata aaaagaaaac atcggatgcg
    25501 ccgccgtttt ttcatacact ttggtgaccg atttgatttt ttgatggtcc ggcgccacgc
    25561 caccgcgaac aagcccatag ggcgtgggaa gccggtcgta catatcaatt tttacgatta
    25621 cgtctttttg ttttaaaaga tgatccgccg cataaaaacc ggatggaccg gaaccgataa
    25681 ccgccacacg aagcggattt tctgcagtac ctaatacgga cataaaatac ctcgtaagtt
    25741 gataaaaaca gcggcgcaat ataaaaataa aaaagacaca gaatgtatga gattcgtcat
    25801 accgctattc tgtgtcttgc ataaaagaac aaaggttatt caaatcagat tcgctctacg
    25861 atcaacgcac tggcttcgcc gccgccgatg catatcgccg ccataccgcg cttcttgttg
    25921 tgacgattca tcgcgtggat tagcgttgtc aagatacgcg caccgctggc gccgatgggg
    25981 tggcctaacg caatagcgcc gccatggaca ttgagctttt ccgaaggaat attgagttga
    26041 ttcgcggcag ccatacttac cacggcaaac gcttcattga tctcaaagag atcaatatct
    26101 tcgattttct gattggcttt ttgcaaaacc ttaccgatgg catcgatcgg cgcggtcgta
    26161 aaccattcgg gtttacgggc cgatgaagct tgcgccacga tacgtaccaa cggtttgagt
    26221 ccgagtttat ccgctttttc gcgggacatc acgaccaatg ccgcagcacc gtcattgatc
    26281 ttggaggcat tggctgccgt gatcgtgccg tctttttgaa atgcaggttt gagtgtcagc
    26341 attttttcaa aatttgcttt tccgggctct tcgtcatcgg caatcacaac gtcgcctttt
    26401 ttggaggtca cggtgacgcc tacgatttca tctttaaata caccttcttt gatggatttt
    26461 tgtgcgcgtt cataacttgt tttagcaaaa gcatcctgcg cgtcccgcgg aatattgcat
    26521 tcttttgcac ataactcggc cgcactaccc atatgaaaat tattatacac gtcccataaa
    26581 ccgtctttga tcatggcgtc cacgagctgg ccatgtccca tacgatagcc tgtgcgggct
    26641 ttttccaata tgtacggaat gttactcatg ctttccattc caccggccac cacgacatcc
    26701 gcatcgccgc attgtatagc ttgtgtggca agcatcacgg ccttaagacc cgaaccgcac
    26761 actttgttga tcgtcataca cgttacgctt tcaggcagac cggcaaacag cgcggcttga
    26821 cgcgccggag cctgtccttc accggcggtg agtacattgc ccatgatgac ttcatccacg
    26881 tcggtcggtt ggatacccgc acgcttaacc gcctcggcta cggctatcgc gcctaatttg
    26941 gtagcgacga tttcactcag cgccccttga aacgaaccga tcggggtacg caccgcgctg
    27001 actatgacta cttctttcat atatacactc ctgattgctt tatataatat attgatttaa
    27061 caaactagta cgcttttcca agcggtctat gttctctatt cgttactcga tttgattgac
    27121 aaattttttc gatgtatcgc gccggtctgt tcatcatcat aagcatcata cgcatttatt
    27181 atatcacgta caagtttgtg acgcacgacg tcatttttgt caaaataaac gaacgaaata
    27241 ccttcgatac ctgttagaat ttctttcact tcgcgcaatc ccgacatcat accgcgcggc
    27301 agatcgctct gtgaaatgtc tccggtcaca atggcacggg cattggcgcc aagtcgtgtg
    27361 agaaacattt tcatttgtgt cggcgtggta ttctgcgctt catctaaaat cacaaaggca
    27421 ttgctgagcg tacgtccccg catataggcc agcggtacga tttcgatgac atgatgcgcc
    27481 atataatctt gaagtttgtt tgatggtatc atgtcgtgta acgcgtcgta cagtggacgt
    27541 aaatacggat ctattttttc atgcatatca ccgggtaaaa acccgaggct ttcccccgtt
    27601 tcaacggccg gacgcgatag aacaatacgg tccacttctt tattctttag cgccgctacc
    27661 gcaatcgcca cggcaagata cgttttgcct gtaccggcgg gcccgattgc aaaaataatg
    27721 tcgttttgcc gcgcttcacg gatatacgat ttttgtatat ccgacttggc ctcgataaaa
    27781 cctttatgag taaacaaaac gacggcattt agatttttat gcgtaataac accatccgat
    27841 tcgctcgaat gctcatcccg gccggttttt acgagacgta tcaccgtttc cacatccacc
    27901 gaatctaggc gtgatttttt attagccaaa aatatcaatt ccgtaaaaat ctcttccaat
    27961 tggtgaactt cgctcgcatc gccgcggagt gttatatttt caccgcgcac tacgatccgc
    28021 gcagtataag cacgctcaag tattttcagg ttgatgtcat tgggccccaa aagaaccagc
    28081 ggactgattc ctttgatttt gatactacgt tcttcggtat gaggcaaaat aactccttaa
    28141 caaagaaaaa gctcccgaat gcattcatcc gggagctttt tgtttacagt tttgacacgc
    28201 gtgtcattcg ccagacatta gttcttagga atgatcagga tcgtgtgcgg atagatcact
    28261 ttggtatcag cgcctttatt gatgttatca ataacgtttt tattcgcgtt atagatcttc
    28321 caccagtcgg acggattacc gagacgcgat tgagcgatcg acttcagatt atcaccgcgt
    28381 tgtacccagt attcattggc ttcttcggaa cgcggaatga taaacgtctg attggcatag
    28441 atcaaatccg gatttttgat ttgttcgcgg ttagcggagt aaatttttac ccattggaac
    28501 ggatcggaat acacttcctt tttaccggag atgcgccaca aataatcacc catgacaacg
    28561 ctatagctat catgacgcgg tttgggcggc gtatagtttt ttccgcggct gatcacctga
    28621 tccacacggc tattgagatc attgacttta ttatatgttt ccgtcaatgc ataccgcttg
    28681 tcttttttaa gcgccatggt ctcggccttg agtgcttcga gttctttttg tttttcaggt
    28741 ttgctcaacg cttcgtcgct cagttttgat tgctcagcaa ctttagcttc aagatcagcg
    28801 atctgttttt tgtacgcttc aacaccggct ttatccgtac caagggcggc atagatttcc
    28861 tgccaaactt gctcggtttc ttgatctact ttggtgcctt cttctttgac accgttgata
    28921 gctttattca agctgtcaat cgtgcgctgt tgagcggctt gctgttgttg ttgcgcagca
    28981 acttgggcag cccaatcggc tttttccatc ttgacttcat cttgcgcttg tacggttgcg
    29041 cctgtgatca ggatcgaaag gccgagcgcc aataaggatt tgattttcat tttgataagg
    29101 tctcctcggt cttcgttact tattattctt ttcaatttct tccaggcgct tcaatacttc
    29161 agatttttct tttttggcct gttctaattt ttgttttttg gtctgcactt cctgttcgac
    29221 gcgtttcttt tcattcgtca gctcgacaac tttgttctcc atggacttgg tcttggcttt
    29281 gttgtcatct aactgctgta attcttcgcg cgtcgcgtag cttttgcagc tcgccatagt
    29341 cagcagcgca aaaagaccgg ccaccaacaa cgccttcgat ttcaacatag tgagttctcc
    29401 ttcgtatagg atttataagt actaaaataa tatagatttt aaggctctat gggtatagta
    29461 cgtatttcaa cggattttgt caaataaaat aagaattttt gtttattcat actttaggtc
    29521 aatgatatta ttgctaccgc aggtcgaaca tgaaaattta gggtattttt cgccatatcg
    29581 tatgatgtca tcatgctttt tgttgcatgc tctgcaccaa tagcgtatag caattttttt
    29641 ttgccaaatg atcatcagtg tctttatcac aagtatttta aacggagtct ttacgtgttg
    29701 cgccatacat atcccctctt ttttaaataa gcgccggttt agcgaatcgt attttcgaaa
    29761 ccaatcaacc aacggaaagc ccatgatttg ccggactgcc cggccagcga aggatcgctg
    29821 acccataaag gaaattcggc tttaatcgca taattaccca tccacgaagg aataaacgcg
    29881 ggcagatagc gaaatccaca gcctgcatca aaaaccagag agggtataga atgtcttcgc
    29941 tttccgcttt taccggatac accgccggcg tcaaaaaaca gtatcggttc aaattgagta
    30001 ataaataaaa tttctttttt tattattggc tttagagggt tagccggttt caaatccaga
    30061 ttaatcgcga tgatatgcat acccaacgca ttgctatccg catatccggc catactgccc
    30121 tgaccgtcgt aataaatatg cctttgaccg gacgcatcac gccagccttt ttcaggcagc
    30181 gtacctctcg atcggtacca aggattgaaa aacatttggc gtggcgaagc gccggctaga
    30241 taaaactgat cttgcacagg tacgtttccg tcagcaaatc cggcaaagat tcgactttcc
    30301 agcgtagcca catttcgttt taatacccaa ggcctgtaat atgtcaaatc ggctttggta
    30361 aatgtataat ccgacgcaaa agtcgaggtt tcgacggtca ttttcagacg aggactgtca
    30421 aagatttgag gctgccattg taaagacgcc gtcaaaacat ggacatcccc tttttcccaa
    30481 aatactcccg gattcaaata cgccgaatta aagcgacgcg acgtacgaag acccaatgaa
    30541 acgtcataat acggaggacg ttcgtaagcg ccgccgaacc acttcttacg aaacgataat
    30601 tcattctgaa acacgccgtc aagtttgtat gatgccatgt caacaaaaac ttgcctgcct
    30661 atactataca tcggcgtact atatgttatt tcataatcaa tagtcctgct tcgggtgcct
    30721 gcgtacaccc ctgcttttat ttggtgttca tcggtggccc atttgcctct cattttccaa
    30781 ccgatgcggg cggcatccgt ctgattgtac cacaaagacg gccgatgttc gatcacgtac
    30841 gtatcagacg tcggtaaaat acctagcgga cgttgccagt gccactctgt tttcgggagg
    30901 aaaccactac gattgttcat tcgaaagata tccggcagcc gtccggatgt atcaatttca
    30961 gcacggtcca ccggcaaatc agttatcatc gtcaacgtat acgtcggatc cgatgcaggc
    31021 cattcggtcc atcgtttcaa ccccgactta ttcgggtcat cgtggcgcgc cggaatgcgc
    31081 acgccgctcc agcttccgtc ggtcagtttc agatacaaat ccacaggcat tgagattgcg
    31141 ccgtgacgga ctaatttgac ttttacctga ttttgaccgg cggtgttttt agaattattg
    31201 atgtgcgcta tgccgtaatc gcaatatgtt gtcgtattga gccattgatc aaataacgaa
    31261 tctaatttct caccggtttt atactcgaga aaacgcttaa aatcgtctgt cagcgtatga
    31321 tgcttttggt atacttcaaa aaaatcacgc atgagactgt caagacgttc acgcccgtaa
    31381 cgatattcga gcgcattcat aaaaatctgg cctttgtaat acgttgccat ggagtacgac
    31441 tgatcgcttt tgaagtagtc cgaatgcgtc aacacgggtt cctcgtaacc ctgctttacc
    31501 caatgcgccc aaccagtaaa cgtagcaata cgcgcatcct cgcgcggaaa ccattttttt
    31561 tgaaattcgg tttcgtattc cctattggca taccggccaa atagatgctc cataagacgc
    31621 gtcgtataat aagacgttat gccctcatca aaccaggcct catgtgtctc atcgttagcc
    31681 agcaacccat aaaaccaatt gtgaatcaat tcgtgtgccg tgacactggc aagactaaaa
    31741 tttcctcggc gtccggtaat aaaaacaata tttggatatt ccatccctcc gtcgccggcc
    31801 tgcgcaacgg tcagctccgg ataaggataa cgtccaacat gcgcttccat gtaatcaata
    31861 atcttcgtta cccattcctt cataggagcc catcgttgtt cgacatcagg cagacacaat
    31921 aaattgacac gcatgccccg atgatcaaaa atctccactt tatagtcggg atcagcagca
    31981 aaagcaaagt catgtatttc ctctgcgtgg tagcgccatg tacggtttgg cattgtcacc
    32041 gattgctgtc gcgtaatttc agcgagtttg cgttgcacgg attcgaaacc ctccggtatg
    32101 gaatcccggt tgccgttgga tgataaaacg cgatccattg aagtactttc gggttcgctc
    32161 atcatcatag catggatgtt ctcggcattg gtcagatggc cgctggcgcc gacgatgtag
    32221 tgtgcgggca aggtgatgct gacgtcccac gtggcaaatt caccaaaaaa ttcacgagca
    32281 agataataat tcttatgcca tccgcgatga tcataaacgc acaacttggg gtaccactga
    32341 gctatggaaa agctcacgcc ttcccggttg aaacgaccca tacgccgaat ttgcgcggga
    32401 attttcacct caaaatccag cgtcagcgta caaaccccgc ccggttgaat aggctccgct
    32461 aaagttattt ccagtaacgt atcgtcttca cgttgtgata agaggcgacc aggttcgtcc
    32521 cgaatatttt gtactttgct ataacccaga taggcctccg gtacatacga gatcagactg
    32581 ccgccgtaat atttatcccg ctcctcgtgg taacgatgca tacggctgtt tttacggaat
    32641 gcattaacat agagattcat atgcaaagcg tgcagtgtat cgggcgaatt attggtatag
    32701 ataatttttt gtcggccggt aagcatgccg gtcgccggga cgagatcaac atccattaca
    32761 taatgtattt tttgttgcca tcgcggaaga taggactggc ctatactacg cgcggcttgt
    32821 gaggcgaaaa aaaatactat ccctaaaata aaaaagcctc gcgaacggat cgggagactt
    32881 ttcaaataca aaatatgcat ataaatattt accggcgaac ggacttcatt ttttttctaa
    32941 tttcaaacca acccatattg gtaaaaccga aatcggaggt cataagtact tttttcaact
    33001 gccaagcgct caaggtcgga tattgacgca ccgcacgtgc aagatattca tccggggttt
    33061 cgtcgccttg ggttgtggcc ggtgtattat cttcttcgtc ttccgcctcg tcggccggtt
    33121 tcgaagcctg tttagtaaca ggcgccgcgg ttacgggttt ggcaaccgat gccgcttgga
    33181 tagtttgagt gctgtctttc ggtttagccg acatggatcc gccggactct ttgacaaagg
    33241 catccacggc ttcgtccagc gtatcgtacg ctttgagaat ataatgaaac tcgagtaatt
    33301 caaaaacttc atacacttcg gggatcatgc gaacgagctt aagatcaccg ctgttttctt
    33361 tgataccctt gatttcagaa ataaaaatac cccagcctgc ggaactgata taatccacat
    33421 cgccaagatc taccacaata cggtgttggt tctttttgag caaagagcct agcgtacgtt
    33481 ccagttcgga tgacgtcgtc gtatcaatgt accctccgac ctttataaca gcgacgctat
    33541 cgccaggacc ggcgaacttg accgtagttt gaataccctc catgtgcgga aatcctcttt
    33601 agaatctgac ggtttgtttt ttttgcaatt gatatcaact taatccaatt tgcttccatg
    33661 cgcaagtttt ttgtttatgt aattttactt agttttccat ttgtcttttt tacaaaaaat
    33721 tcataaatta caacaattaa actgcgccga ttgaataata atattgtgac tggcattgcg
    33781 atttcggttt tgagatatac cacggcatac tccatgtttc tcagaacgca ctatgtctga
    33841 actacgtaaa gatccgatcg tcaatcgttg ggtcatcata tctgaggaac gcggaaagcg
    33901 tccgtcagat tttgcagaga cgccgtcgca attaaccaca agttgccctt tttgtgccgg
    33961 taacgaacat ttaacgccaa gcgaaattat ggcttttcgt cacgaaggaa ctatgaaaga
    34021 tcaacccggt tggtctctgc gtgtagtgcc taataaattt cctgcgctga tggtagaagg
    34081 tcaggagagc caccggcata atggttttta cgaatttatg gacggtttag gtgcccatga
    34141 agtgatcatc gaatcgcctc agcacctgtg tacgctggaa gaaatgagcg caccggattt
    34201 taccaatgtt atccgcgctt atcgtgaccg tatccgcgac ttgcgcaatg atacgcgttt
    34261 gaaatatgcg ctcatcttta aaaatcaagg tgcgcccgcc ggagctacgg tggagcatac
    34321 gcattcgcag cttatcgcaa caccggtcgt acccaaaact gtcgaggaag aattgcgcgg
    34381 ctcgcaagac tttcatcaaa ctcataacca gtgcgtattt tgcggtattt taaatgatga
    34441 tcgcaaagat aaccaacgca ttgtattaga ccgcgacggt ttcatcactt tggaaccttt
    34501 tgcagcccgt tttccgtttg aaacatggat tttaccggca actcatgaag tgcgttttga
    34561 agatatgaat gacgaaaaga ttaacgctct ggcttcggtt tttcaggaaa cactgatgcg
    34621 ccttcggaaa gcgttgaacc atccgcctta taattttgta ttgcatacgg cgcctctcaa
    34681 tacctatgtc cccgatcacg cgtaccattg gcatatcgag attattccta agttgacgcg
    34741 tgtggccgga tttgaatggg gctccggttt ttttattaac cctacggcgc cggaaaatgc
    34801 ggcgacgtat ttacgacaag ttcgacttta atggagtttg tttaggatat gaaaaaatcc
    34861 aaaatttttt ttgtagcacc ggcggtttac ccttttgccc gtgacggacg cgtagccgaa
    34921 tttgcggccg gattgcctgc acaattgcaa tctatggggc atgaagtgcg cgtacttatg
    34981 cccaaataca gtctgatcaa tgagcgaaaa cacatcatcc gcgatattat tcggatgaaa
    35041 gacattcccg taaaaatgta taacggcgat ctgccggtgt ccgtcaaatc gggcttcatt
    35101 ccggaaacca aaacgcagat atattttgcc gagcaagaaa aatactacaa acgctccgat
    35161 ctctaccgtg ataaacgcac gggcgaatat tataaagaca acgacgaacg ttttattttg
    35221 ttggcccgca cggctatcga aaccttaaaa actctgggat ggcagccgga tatcgtacac
    35281 tgtatagatt ggcagtccgg ccttttgccc gccatgatcc gcgaagagca gaagcaaaat
    35341 aattttttca aaaaaacgat cattacattt tccgttatta acacggaaga acccgctttg
    35401 tttgacaaat ccgtttttga caaggccttc ggccaggcgc acatcatcga aaaagaaaaa
    35461 tgctttcaca aaagcaaatt cagttttctc aaaacagcta tcatgcattc cgatgccgtc
    35521 acgataccga tgacgcataa acatctgaag accatcacca aaccggccaa cgatgtggag
    35581 gccattttgg catcggccaa aatggtgcag gagtcttatt ttggcggaga gcacaattat
    35641 tggagtccgg tcaataataa taccttatac aaatcctatt cagcggaaaa acttgagcga
    35701 aa
//
LOCUS       DAOQGF010000022        35425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_536_length_35425_cov_6.419056, whole genome shotgun sequence.
ACCESSION   DAOQGF010000022 DAOQGF010000000
VERSION     DAOQGF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 35425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_35425_cov_6.419056"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PK937_05105"
     CDS             <1..990
                     /locus_tag="PK937_05105"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH31980.1"
                     /translation="KTSAQMQDKIGSRYAIIYKEEDRSKLIKHSHKFHATKGGAECKD
                     CHASITSSEKAMDSNLPKMDACYECHDKETTDCKLCHLEPKEPYSSYKSPKRELTFNH
                     KAHLGMKNVNCETCHSEITNKAYTGARTLPAMESCMGCHDGAQASNDCKTCHTDIRFI
                     KPADHMAGFMRTHKHEVATQGDANCRMCHSTESCEDCHNGGNLNKMKNRNEFSPSGTP
                     SGMSENSSQIISKAHALDFIFTHRFDAKAKTSECQTCHEAESFCVQCHNNNDKMSKPA
                     WHKVAGFTSGVGGGLHAQLAKKDMENCVSCHNIEGQDPTCYTCHKADGSLKNF"
     gene            1025..1381
                     /locus_tag="PK937_05110"
     CDS             1025..1381
                     /locus_tag="PK937_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31981.1"
                     /translation="MLSLLAFVGCSESEDIGNVPGPPDPPKPYSYVTDIKAVLDEGCA
                     SCHSGGSPSGSYNLSTYAGVLGNGSDATPNAIPGDSTSLIILKLKSGHQTPDASTISM
                     IFQWVYQDSCKETLPD"
     gene            complement(1439..2827)
                     /locus_tag="PK937_05115"
     CDS             complement(1439..2827)
                     /locus_tag="PK937_05115"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNH31982.1"
                     /translation="MQHSIVWLRRDLRLDDNTALLHAMKASQFVSLIFIFDKHILDRL
                     PADDRRLSFIYESLTYLQNKLKAHGAPTVHIFYGEPEVVIPQLFSENKTDALFFNHDY
                     EPYANVRDTAITEVLNQKGILVHSFKDQVIFEKSEIVKDDGTPYKVFTAYKNRWLNTY
                     THSYPNAITDSTSIPWKKFSPLFSSLPLIHSLQEIGFVQCENIIHGGSDAAQKQWTQF
                     TGDYIEKYDTQRDLPGLNATSHLAPHLRFGTISVRQLIDYLSKHEAPAVKVFLSELIW
                     REFFMMILWHFPHVIIRSFQPQFDRIVWSNREDWFEAWKSGHTGYPIVDAGMRELHAT
                     GYMHNRVRMIVASFLVKHLHIDWRKGEAYFAEKLLDYELSSNNGNWQWAAGTGVDAAP
                     YFRIFNPLTQGKKFDPDAVYIRRWVPELENVSLREIHDPIKRQSGLFSHYPHPIIDLD
                     QERQKCLALYKV"
     gene            3087..4919
                     /locus_tag="PK937_05120"
     CDS             3087..4919
                     /locus_tag="PK937_05120"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNH31983.1"
                     /translation="MMYKNLSDCIADLERNGHLIRVNEEVDPYLEMAEIQRRVYQKQG
                     PALLFTRVKGCRFPAVSNLFGTMDRTRFMFRHTLAAVQKLIELKVDPANLAKRPLRYA
                     GVPFSALTMLPWKKSNGAVLKNTCRIGELPQITCWPKDGGPFVTLPQVYSEHPDHPGW
                     KMSNLGMYRIQLAGNQYIQDREVGGHYQIHRGIGVHHAAAIRRGEKLRVSIFVGGPPA
                     HTLAAVMPLPEGLSELMFAGVLTGRRFRYGYQNGHAVSMDADFVITGTIDTQATKPEG
                     PFGDHLGYYSLTHDYPYIEVEQVYHRDGAVWPFTVVGRPPQEDTSFGDIIHEITGPVI
                     PTVIAGLHGVNAVDGAGVHPLLLAIGSERYVPYGERKPQELLTIANAILGQGQLSLAK
                     YLFITAKEDDPSLDVHDIPKFLQHVLSRVRWEEDLHFQTRTTIDTLDYSGFGFNQGSK
                     VVIASAGKVRRLLASQVPSELKLPEGFSDAHVILPGIVAVRGAQYSDRVAARKAIRVL
                     CEYLEHQRDLENLPLWIVCDDVTFVKAQLNNFLWVTFTRSDPAADMDGAGAFVEDKHW
                     GCRGPLVIDARIKPHHAPPLIEDPDVTRRVDALAEKGKSLYGII"
     gene            4957..5751
                     /locus_tag="PK937_05125"
     CDS             4957..5751
                     /locus_tag="PK937_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNH31984.1"
                     /translation="MEINCLHIADIFAQSGLDMVTETLPKLIKERDIHFVVANGENTL
                     EGKGITEGQCQHLFNLGVHVITSGNHIWDRKKTFYAENPLIQKYLLRPLNYPVGNEGR
                     GSTVYELKNGLKIGVINLQGRTYMYDIDCPFRVGEREIEKLQDETNIIIVDFHAEATA
                     EKIAFRHYVDGRVSAVIGTHTHVQTADEMITSSGTAYITDAGMTGPHDGVIGMQKEPT
                     VKKFILQTYFKFEPSKGDVRFHGLFFKVNAETGRATYVERVRIDKS"
     gene            5866..6237
                     /locus_tag="PK937_05130"
     CDS             5866..6237
                     /locus_tag="PK937_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH31985.1"
                     /translation="MAKICVIEDEPNLRLLYRSEFERMGHEVICHANGREAYRSLPVD
                     QPDLIILDIMMPEGDGLEFLSNVLVMHVGIPVIINSAYAQFKNDFVSWAAEAYVVKSS
                     DLSELKEQVHRVLFKFSMATA"
     gene            6406..7263
                     /locus_tag="PK937_05135"
     CDS             6406..7263
                     /locus_tag="PK937_05135"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNH31986.1"
                     /translation="MEIKRIAVIGAGTMGNGIAHVFAQTGYDVNLIDIKQEFVERGKA
                     NIEKNMGRQVTKGTLTEEQKNAALVRITLCTKIEDAANCDFVVEAASENPEIKAGIFK
                     TLDNVCKPDVILSTNTSSISITQIAAVTKRADKVIGMHFMNPVPVMKLIELIRGLATS
                     QTTYDTVKTMSEKLGKVPVEVNDYPGFISNRVLMPMINEAIFCLMEGVASVEAIDTVM
                     KLGMNHPMGPLQLADFIGLDVCLSIMNVLHEGLGDDKYRPCPLLKKMVTAGYYGVKSG
                     KGFYDYPKK"
     gene            7338..7778
                     /locus_tag="PK937_05140"
     CDS             7338..7778
                     /locus_tag="PK937_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31987.1"
                     /translation="MRILVMLVSLIVGASLVAQQRTTIKFGTGKIDMTCSNKAAAGFY
                     KNIEIRSTDTPIRIYFMKNDKTVMDVSGSAVYISYEQDTRGDDVAAVTVDQKNFWGVK
                     VIYFVTATVPNKYVDINGEQKEVAGHLNLDYLGLPLWISRTSKS"
     gene            7947..8675
                     /locus_tag="PK937_05145"
     CDS             7947..8675
                     /locus_tag="PK937_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNH31988.1"
                     /translation="MRFCFLMLCTLSFTGCDKEPVTEDVQITRGRELFHSLQFSSDGK
                     TSCATCHPEGHTDNRNWHFAAIHDTTAGIPDSFRTITLWGIAQTGSPYLWKNSFRSLE
                     SVTRLYTDTIMGGTASEDEVAALVAYQRSLAFPKNPNLADNGQLTEAQARGKSIYEDP
                     KRGNCAPCHPAPTYGSPIPKNIYTGGTFRTVGLRGLYSIAPYFHNGSAKTLHEVIEHY
                     AGNGLIAPLTAQERDDLVEYLKTL"
     gene            8697..9503
                     /locus_tag="PK937_05150"
     CDS             8697..9503
                     /locus_tag="PK937_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH31989.1"
                     /translation="MKHDKMRFVLILSLIFSVMQSCEKEKINEDAQIAYGRKLFNDTK
                     FSGSGQLSCGTCHPQGDMDNEQWHLDLIHDSLGGVAVSYSTPTLFGVAETAPYLWKGQ
                     GGSDLDTLTKTVITGIMGGTATDEEIKAIGAYMRSLDVPANPFRNDDGSLTTEQARGK
                     IVFENQGKCNICHSGETLSGKFSIQIKAARPAFDVPSLRWVFATAPYWHDGSAGSLRA
                     VINHYADTITTAQMTTWKWNGAGQVTPKPIFDIELTEQEKNDLIEYLKTL"
     gene            9528..10736
                     /locus_tag="PK937_05155"
     CDS             9528..10736
                     /locus_tag="PK937_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31990.1"
                     /translation="MSKFCWIVIAVCLLAAPIHAQEETVAPNTESGTEGTSVGFGGFV
                     DVAHAVGRKAGNANTLGDNNFNNMRTLLMFNAQQGSKIRADVEVLFDDRSKDRIRLQG
                     AFVTIFDVPNEAVNFMIGKIPNPFGNFAKREFSDVNPLIGQPLMRQYRTALDWNNLWN
                     NAEQIARKSERKKYDGNIPISRFEAATPTVYDARWDFGVQMFGTIGWFEYQAAVTEGS
                     ISNPEADLNKGKQFVGRIGFNPVPGIRIGFSGAYNPYLSKADDQRLLEEGKGLGEYHQ
                     TAYGADWELSYRYFMMSGEFVRSTWDATVIEGELSNWSIYSDIKYKLHPRWYIAGRYD
                     IMRFSEIRESKLSSKKTPWDYNVQRYEAGIGFRVTTDVTLKLVEQWTRFDQSEIPMSR
                     LTAVQLSVPF"
     gene            10757..11479
                     /locus_tag="PK937_05160"
     CDS             10757..11479
                     /locus_tag="PK937_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNH31991.1"
                     /translation="MKLIFSAIAGITFWILSVTGTSTRLFEAMDQNARLNHDVMLMRP
                     AGDAKITGTVSFPRSANATGRAVKGSYGKNTSRLKSGSAGEKRAVVYIASAKGNFPAP
                     QSRPAMRQKNIAITPRILPVLAGTTVDFPNEDDLFHNIFSLSPSVKFDLGRYAKGVSK
                     SVKFTTPGVVKVFCDIHSTMGGVVLVLQNPYYTVTEADGSYALSGVPAGTYDLVAWHE
                     GGTQTQKITISASGVITQNFAF"
     gene            complement(11685..12029)
                     /locus_tag="PK937_05165"
     CDS             complement(11685..12029)
                     /locus_tag="PK937_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31992.1"
                     /translation="MPSLRATHEIFKTHVLLVLEGRFNPYDSDDDLVVLQNAYDLIRK
                     ETGQTDLILEFDMQHIGSVGINTLIKAYQMARTNDGRLIFIQFPDSEILYLEITGFNR
                     YVSCLQNRTELF"
     gene            12107..12640
                     /locus_tag="PK937_05170"
     CDS             12107..12640
                     /locus_tag="PK937_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH31993.1"
                     /translation="MKSNRYWFVALVLIGFISSCQSSKTETAADLFKQADSLFTKNKT
                     DEAFKVLAEMETRFKSDTTVMCEVWRVTAEAYAQYKNEFSKTIEYYDRIVNTYPNTPQ
                     AVASLFKKAYTYENGLNDNDRAKAAYEEFIKKYPNHELADDAQTMIDFLGKPADDLIE
                     MLEKKAKQNEKKSTPAK"
     gene            12655..14085
                     /gene="pyk"
                     /locus_tag="PK937_05175"
     CDS             12655..14085
                     /gene="pyk"
                     /locus_tag="PK937_05175"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNH31994.1"
                     /translation="MINLFETRAKIICTIGPSSSDYETLVRMIDAGMDIARLNFSHGS
                     HDAHRQVMDNIRKAAAQVGKQVALLQDLQGPKIRVGIFKNGPIELHIGEKFTITSRDV
                     DGDQHIVSTTYKELPGDVKVGDDLLLDDGLLRLRVVSKTGTDVVCEVIIGGVLKNNKG
                     INLPGVHVSAPSLTEKDAEDLLFGLANDVDYVALSFVRKPEDILHVKEVIRAHGKNTP
                     VVAKIEKPEAIDCIERIIAITDGLMVARGDLGVEMKTEEVPPIQKRLIHLCNRAGVPV
                     ITATQMLDSMVNNPRPTRAEASDVANAILDGSDAVMLSAETASGKYPVETIATMKQII
                     KLIEKEIPHDQSKRRKTGVELQMMQEGIAIAACGLADMLEADAIVNITMTGAMSKLVA
                     KHRPGTPIIAITHSEQVCRQLNLVWGVRGINVADLKEDIDESIEKVKKALVKRRILSA
                     GQRILITAGLPFGGRGATNTVRVETV"
     gene            complement(14100..15809)
                     /locus_tag="PK937_05180"
     CDS             complement(14100..15809)
                     /locus_tag="PK937_05180"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HNH31995.1"
                     /translation="MINRLFLFIVLTTTGYALAQGHLSQTCKDHADGLISDQQAYDTL
                     FDLLTASDSTPIHEADKCGFPAIQTLQTLSQKLNKSFTVQKKSAAFVYNHAFVTGSGK
                     NRTFKIYYDTVGTDKVSTLDADANTIPDWVEENGKAFEKSYRKEIDTMGYREPVNMKR
                     DGYYEVYIINIRAYGQTAPVQQYPSGSDTWESFIEIENDFVGGGFKTHGYDAMRVTAA
                     HEFHHAIQLAYKYDTGYTDGWFYEMTSTWLEDVVYDEVNDYYAYLPRFYSNPHVSLFS
                     ETTFFGYHVTHWLHMMEKTHGASIVRNIWERMPDERALIAINNALEAETSTDLRNSFT
                     EFSIWNYFTKDRYDSITYFQESRSFPAVSFYKNTLIGNLTYTDGIPGLAARYYNFKTF
                     DSSDIKIRFESGSPSDFNIITIERLESGDPFITRRSHVDSFVVSHMKAGDSLIVIVSN
                     TTTNKTNTTYTISTGLTDGSSSHATVTNLQIYPNPIRMDLHNTLNIKFDLRKTASVVF
                     EVYSISGKRMVHHNYGKFDPNNHLLNWDIKDNAGKFLPSGIYIYRLNIGDYSKTGKIA
                     VVR"
     gene            complement(15806..17176)
                     /gene="der"
                     /locus_tag="PK937_05185"
     CDS             complement(15806..17176)
                     /gene="der"
                     /locus_tag="PK937_05185"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNH31996.1"
                     /translation="MASKKINRLPVVTIVGRPNVGKSTLFNRIIGRQDAIVDGTPGVT
                     RDRHYEIAEWRGKNFILIDTGGFIPDSHDLINTAIREQAQLAIDESDLVILLIDAVSQ
                     LSTTEMKIADVLKRSKKTSLLIVNKVDNEKVYQNTLADQEIYKLAMGQPLMVSALNSR
                     HIGDMLDRIMTALGDRFAANPDEITTDTNTIRLAIIGRPNVGKSSLVNAIIGKNKHIV
                     SDIPGTTRDAIDTDFIYNDTAFKLIDTAGLRRKAKVKENLEFYSTLRTLKTIQECDVA
                     ILLIDAIDGLAAQDIRVLEEARQLKKGLILVVNKWDLVKKTDKTALDYEKHLDKALGA
                     TKYIPYVFTSALTKQRVAKVLETAKKVHDERNRTIATAQLNRFLERIIARNHPPAVRG
                     KEIKLNYATQIKSRPPVFAIFSNEPTLIPANYRQYIENQMRDEFGFEGVPLSLTFRKK
                     SRDRYE"
     gene            17261..19129
                     /locus_tag="PK937_05190"
     CDS             17261..19129
                     /locus_tag="PK937_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH31997.1"
                     /translation="MYIRSLQIRDFMGFSLYRTEFKEGFSALMMGPSQGKTGFRMAMR
                     VVLTDHFDRNFALFHRNHSGTSEINLELYCGKSALRFNKTWTDGAVQQWTVENPDAGT
                     VLDLTNQGTYDDWVRWFLYETQGWPVSEHDGETVLHNEWARGQYDFLMRLIFCDDTTM
                     TDLMTIPISSLVGLRYVQNMEVRLNELIQKASEKVFILKSEENLVQNELSLLEQKRQM
                     VLVLEKDLESDTELLQKLDERLIQTQHERVQAERITGYIQEKRSEITKLGFEITTYQN
                     VMQNNVLQDTSEEEIRTMDRKARLYEKAIKEIETIEENIIQRRHLEKNLEGIEQEIQV
                     IDRTHTGKLPPEIKTQKKAYESEARRLRLLLSKMQDDPSSMEHWQTEKRAHEEAYHQY
                     TILKKTRQYMNQNNLQKLKSTTDKLIVKRQHMQDELDQIFASQHETQYQKLEAAVASD
                     RQQALELKERMATAVGRKEKLQQDIQALQLVVDKHLSRADQKRREITSKRHLLFVRET
                     VTDFYNAQIELRKNELSRQIKTHLKKFRVAFTSKQLFGKNKTNEPSLETIPISTSERI
                     LSEHIFAMTVLAMNLKNPIFFADENFSWYLTKDDRTEIEALADQLNFGQFILFEKN"
     gene            complement(19126..19419)
                     /locus_tag="PK937_05195"
     CDS             complement(19126..19419)
                     /locus_tag="PK937_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNH31998.1"
                     /translation="MIDHIVLFKLKPGADTTAIENMCTALEDLKHRIPQVIEIHARPN
                     FSERRQGYDIMLTSRFKNKEDLRIYADHPEHLRVVKELVAPIRESVIVGDIEF"
     gene            complement(19434..20888)
                     /locus_tag="PK937_05200"
     CDS             complement(19434..20888)
                     /locus_tag="PK937_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNH31999.1"
                     /translation="MMKDYFLITLFFFVISFGRSQDLAENTKPRIRKSETLLVYPPAN
                     HAPIHKATAVHLFAFMALIGRSDITPSDPRGLAVTRLKSTDDPDNRNDDDDLTAYGVN
                     SGQNNIIFNSSMQGLDVYEGKGPQQKLKRPRGIACNSDGDVYIADTGNNRLVKLKNPG
                     GYLLFVKASGKKGNKSGEYDEPRGVAVDQKNQVYVTDYKNDRIQVLDSNLIPCQIWGN
                     ATDASGTLSLYRPDAIAASDGSDPDNFTQDHFLIVVDMDGTRIQKLGFDGHFQMGVVS
                     SEYGFNKVNLTSVAIDGFNNVWVTDAENHAIHKFDRDLRYMGTFGSPGDGENQFIEPR
                     AIAIGKKFGQVFIADKISAQYYHIGTDIQNLNVAIKDSQLVFSFYLPETSRLTARIVD
                     EKGKLVATLARNSMTSPGIKMLQWNRAYKSNSPLRDILQFREQNRSDSTQGKQPAMTE
                     QLAPSGIYKIEIEAKSTYIYNRYFTAKRALEFAF"
     gene            complement(20885..22864)
                     /locus_tag="PK937_05205"
     CDS             complement(20885..22864)
                     /locus_tag="PK937_05205"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNH32000.1"
                     /translation="MKNIYYLWALTWLCAPGIWAITSDEYGKVIKELAAKIQSSNTTG
                     KPQRLAVVTFVPTKTDAENKNEFGEYITESLISALGRTKQFRLFERRRLDVVLKENAL
                     MMSDLMNSEQAQKVGELIPIDVLFSGTYTKLKNYIDVNCRLIDVITGEIYFSFSGSIA
                     MTADLASLFPGEHNVNAASRAITEAERCKQNADLLKHKLNDLTTSSKIQSAVDEAVRI
                     PFDTECGNVHYDVLYVFTRYKIQNTAYKKFLIQTLGGIANPDQDDRSKETIRYFAADS
                     VIDEEEWRIGLETITRMRNYRLYICLNLLFPTDDLRFSPRVDAYLNRALDNKIGLPKP
                     ISFNHALDHLADGFKDTPTLMSYAYKNYQKHIQWDSQAQKDIYALLNRLYQTLPTSEN
                     KRQVLDWLSEYFHRFDASDDIADRLFDFIRQFRLTTNAERNKEITAAFPESDLKYFVN
                     KTSERIAANAPKTPYKSQKDERIDFCLRYDIAVPGIIPTMDEAKIILSGKDNWDEQLQ
                     TLLMIEKMGMKPKPLESQLIAIFSKRNLEHKETLTEMQSSAIITLGHLKTSAPAAIDF
                     MMASLMSYDYRFSDNAKTALIQIGTSAVPKLISKLQSLTDQDGGLMYIIAEILGKIGP
                     DARSAKTILQQSLGKTNNSDVRYAIESALQAIEGQ"
     gene            23016..23570
                     /locus_tag="PK937_05210"
     CDS             23016..23570
                     /locus_tag="PK937_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH32001.1"
                     /translation="MTSKDFEKQALAHTESLYNTALRMTHNPQDAEDLVQEAFYKAFR
                     SIHQFEEGTNLRAWLFKILVNTHISQYRKNAKDPATTGYDDVEEFSLYSQVRSRAAFP
                     DDAPTDILNQFLDKDIQAAIQALPQQFKDVVMLVEIEGFSYQEAANILDVPVGTIMSR
                     LFRGRKLLQKSLWEYAVEYGYVKK"
     gene            23616..23915
                     /locus_tag="PK937_05215"
     CDS             23616..23915
                     /locus_tag="PK937_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNH32002.1"
                     /translation="MSHVHHGEAGLHAQNHPINCGEIERLLYAYLDGELPLEEVAPYK
                     DHLSHCPPCKAFVEFEEKISHLIKQKCGHNGTDRACVPTSLHEKIQKAIALSKEV"
     gene            complement(23971..25602)
                     /locus_tag="PK937_05220"
     CDS             complement(23971..25602)
                     /locus_tag="PK937_05220"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNH32003.1"
                     /translation="MKVILAQINSRVGDLTNNTERIIQHINQARSEGADLIVFPELSI
                     TGYPPEDLVEKKAFVRKNQEMLDRIREATDGVGVLCGFVAPNNDPEGHHVFNAAALIE
                     NRQIIGIQYKTLLPNYDVFDEMRHFEPAKHKKIFTFRGVKLGISICEDSWNDKDFWKR
                     RIYHNDPIEELFNQGAEILINISASPFHVQKHHLRLEMFRSIAVKYRKPILFVNLVGG
                     NDSLIFDGRSFALNAEGIPIATGKAFEEDRVSLTLPSTQTITMPNPTTEEEIFNALVL
                     GVRDYLHKCGFRKALIGLSGGIDSALVAAITASALGPQNVIGVSMPSRYSSDHSQDDA
                     RDLARNLDITYHSVPIEPMFRSFLESLSGSFQGLPEDTTEENIQARIRGNILMAFSNK
                     LNAMVLSTGNKSELAVGYCTIYGDMCGGLAVISDVPKTMVYRISNWINRDKIIIPKST
                     IEKPPSAELRPNQTDQDSLPPYDVLDAILKAYIEDLLEADEIIKLGHDPETVHKILKL
                     IDRNEYKRRQAAPGLRVTSKSFGFGRRLPIAQGWR"
     gene            complement(25782..26195)
                     /gene="lexA"
                     /locus_tag="PK937_05225"
     CDS             complement(25782..26195)
                     /gene="lexA"
                     /locus_tag="PK937_05225"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNH32004.1"
                     /translation="MEPKIIYDNQQNPGIPVVGKVAAGTPRFSEEISLGHVVSSRYSP
                     EDKKLFALQIVGDSMIEDGIFNGNYIVCRQTNEFVNGDIVIAYVNEEATVKRIYKRGS
                     KIILQPANAELSPIEVDPQYQSFRVGGKVLEVLRH"
     gene            complement(26198..29104)
                     /locus_tag="PK937_05230"
     CDS             complement(26198..29104)
                     /locus_tag="PK937_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32005.1"
                     /translation="MLESFSHDLSFFHNTAQAETNLKSITNQTQPHDIRFLKRLLDEL
                     RPYPNCDDIFNLFERLSRAVIDRIAFFKMLSDTPPTIARLVRLFASSPFIAELMIRDF
                     QYAYFLVRPEYDCRPFDKLEVQQHILRLFHNQTPSLNKVMDALRKIKRREMLKLGIRD
                     YILQDPLEKTVASVSDLAECVLQGALDAQRRDMAARWGNPETPFCVIALGKFGGGELN
                     YSSDIDVMFVYEEEDEYVANEHRLTHHEYFNRLASGLIAHIAKNTSEGQLYRVDARLR
                     PDGDAGPLARCVSSFLYYYEARGQTWERQMLIKARPVAGDLEFGARFIKRLEPFVYPK
                     TFFQSPLHEVAAMKWRIEEQKSSDHLNIKICPGGIRDIEFIVQALQLIHGGQMTEIRT
                     GNTLKGLKQLFSQKLLSPNEFQTLSSAYIFYRKIEHILQIEKDTQKHTLSGTTDEFTK
                     IAILLGYTNGSTLESVLNENLKSVRAIYLSVFEIDNRATSVFSGLISEVCSEEGIRFL
                     QEHHFTDPDRTHRTIRLLAMGQFPKLYGNSVRECLAELLEILLTDVGQTHDPDLTLAH
                     LERIVAAQPFADMLYRSLSHNAEYRKHLVYLCSLAPGLTQIFCERPSYLDWLQIQLPT
                     WLSTPHQGAQAIYRLTKNLQHIRRLGWVQCALTNRNQPISSSEPYLYLSELADQILQT
                     AFSEVFAMKEPIILLGMGKLGGSELSYRSDLDVVWVCSDDADVDHFISQARRVHQRIT
                     DHASEGKIYDMDARLRPEGAQAPLVVTLSRYKEYFSSRALFWEVQSLIKARCIAGSSD
                     LHSQFESWRYEQLRTASRKWNIRHEITTMRERQIREKIKSPADAVYEIKTCRGGLLDI
                     EYLVQGLQLLHGDRLPQSPLQSAVQAINSLISAGLLLVDDASILTTNYYLLRRIEEAA
                     FLLFERKSSRLPSDEKQLAFIANFLDFKNNDAFLMRLEQIKESNHRLFLHYLGV"
     gene            29206..30753
                     /locus_tag="PK937_05235"
     CDS             29206..30753
                     /locus_tag="PK937_05235"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNH32006.1"
                     /translation="MSDKRVEELHRLKAEALLGGGQKRIDAQHKKGKLTARERIAVLL
                     DEGSFEELDMLARHRSHDFGLDKERPLGDGVVTGYGTIDGRLVYVFSQDFTVFGGSLS
                     EVHAEKIIKIMNLAMKVGAPVIGLNDSGGARIQEGVVSLGGYADIFLLNTLASGVVPQ
                     ISAIMGPCAGGAVYSPAITDFILMVKNTSYMFVTGPNVVKTVTHEDVSSEDLGGAMTH
                     ASKSGVAHVAAENELDCLMKIRKLYSYMPSNNMEDPPVIPCDDPADRCEEALKTIIPD
                     NPNKPYDIKDVIKLIVDNGDFFEIHEEYAGNIVVGFARLGGRSVGIVANQPAVLAGVL
                     DIDSSIKGARFVRFCDCFNIPLVVFEDVPGFLPGTDQEWRGIIKHGAKLLYAFCEATV
                     PKVTVITRKAYGGAYDVMNSKHVRGDMNFAWPSAEIAVMGAKGAVEIIFKKEIDASAN
                     PKETEEKLTEEYKEKFANPYSAAERGYVDDVIEPQLTRPKLIRALAMLENKVDTNPKK
                     KHGNIPL"
     gene            30789..31667
                     /locus_tag="PK937_05240"
     CDS             30789..31667
                     /locus_tag="PK937_05240"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNH32007.1"
                     /translation="MPKVICVANPKGGVGKTTTSINLSASLAKFGKRALLIDLDPSGA
                     ASVGVGFTRENATFGVYDLYKHPESLDEAIVRKAIYASGTERLDVMPSNVWTSEEENE
                     LLKLSAKLTRLRIMINFVRNDYDYFIIDCPPFLSNLTLGAFTASDSTIVPVQCGFFAL
                     HALERLMKFYRKVRESINPYLKIEGILITMYEKGTNVSVKTVRDARAVFEDLVFGVII
                     PKNVKIGDAAFQKKPVVTYDSTSAGAKAYIQLAEVILHRDDPQWDIQAHVQTPSEPRN
                     ESQTIPDVVSSSGENA"
     gene            31769..32368
                     /locus_tag="PK937_05245"
     CDS             31769..32368
                     /locus_tag="PK937_05245"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNH32008.1"
                     /translation="MTDTFLPRVLGKIILASASPRRAQILKQVGFDFDVMPSAIEEVS
                     EHHEPVEYAREIAQKKALDIAQKYPERIVIAADTIVCLDGHILGKPSDEEDAWRMLRF
                     LSGRNHQVYTAFAISHRSRNVNMVEHELTQVHFRRLRDEEIAMYIATGAPFDKAGAYG
                     IQDASAIFVDRIDGDFYNVVGLPVTRLYQVLTDTYLEKR"
     gene            32365..33147
                     /locus_tag="PK937_05250"
     CDS             32365..33147
                     /locus_tag="PK937_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH32009.1"
                     /translation="MSNAYYKELSGWLRQARADQHITAVAVALRTRMPLETIEKIEAG
                     DLDFATPVFIQGYLKNYADAIGLDSAAVVREYKVIRAKIAYVTSDRGQVVARATAQHS
                     GNPDELSSEIIPFDPESHAFAPHSELDQAVRKKISTEKWTNIIMMSSAACMVILSLYW
                     VVSDDHSQDEETAANLAKYERKAETFKQAKIEPRKRLSFYFSEPILTDEEINRKLEVR
                     EREQQDSMRLAIYQKNQKWSHGQKVRAITVQEAALDYAQSMK"
     gene            33186..34790
                     /locus_tag="PK937_05255"
     CDS             33186..34790
                     /locus_tag="PK937_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH32010.1"
                     /translation="MLKKLNDMKLRAKLMAGFGVFSAIFITTVTIVFTVKLHTAAVSH
                     QSLILRSFAELTAKNISAGLDFGDKGSVVSAFDALRTHAEFVMVHDVSGENFATFYRD
                     PKIENRIDPVVNEQIGRWREAKKSDWTFDALDMNCIVVPVFNSAGNEIGNLSLGMSTK
                     SINDELQSNIFFSFILLIVTVGVGIGVVYWISGLLVRPLMNIVERFKDIAEGEGDLTR
                     RIHIDAKDEVGELAAAFNQFLNKLEEIMRQVARTSEQISVSVESISKASSDVAGGAEQ
                     QSNQATLVAVAAEEMSATIVQTTANTGDAVRLSQQAEEATDRGKMVVEDTVNGLQNIS
                     SVVSESAQTLLELGKTVAQIGTVIEVINDIADQINLLSLNASIEAATAGDAGKGFAVV
                     ANEVKNLAEDTTRSTTEISQMIEKIQHAMANAIRAMERGTMEVEKGRALGHKTAEAFD
                     EILKASEHVREMITNISVSSQQQSHSAEEISRNIETIAGITRNTAQGVRQIDGTTDAL
                     VNHTSALKTLVGRFKLNEQDRAQNYN"
     gene            34771..>35425
                     /locus_tag="PK937_05260"
     CDS             34771..>35425
                     /locus_tag="PK937_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNH32011.1"
                     /translation="MHKITTNSPRKTIYTDLMQVCEQRGSGYFVLIDPDKKTEAETLQ
                     TVSHCAEAGVDAILIGSSLMLSSRFGSTIQAIKKEFSLPVIIFPGSTIQLSEYADALL
                     FLSLISSRNPEFLITTQVLGAPIVRKMGLEAISTGYMLIESGKVTSAEFMSNSKPIPR
                     DKNDIAVAHALAGEYLGMKTIYLEAGSGALHSVPAEMVSAVSEYVNIPVIVGGGIRTP
                     "
BASE COUNT         9892 a         7382 c         8498 g         9653 t
ORIGIN      
        1 aaaacttccg ctcagatgca agataagatc ggcagccgtt atgcgattat atacaaagaa
       61 gaggatcgct ccaagctgat caaacacagt cacaagtttc acgccactaa aggcggcgcc
      121 gaatgtaaag actgccatgc ttcgatcact tccagtgaaa aagcgatgga cagcaatctt
      181 cccaaaatgg atgcctgcta cgaatgccac gacaaagaaa cgacggactg taagctctgc
      241 catcttgagc ccaaagaacc gtattcatcc tacaaatcgc ccaaacggga attgacattt
      301 aatcacaagg cccatctcgg gatgaaaaat gtaaattgcg aaacctgcca cagcgaaata
      361 accaacaaag catataccgg cgcacgtaca ctgcctgcga tggagtcttg tatgggttgc
      421 catgacggcg cacaggcgag caatgattgt aaaacttgcc atacggacat ccgttttatc
      481 aagcctgcgg atcatatggc cggatttatg cgtacgcata aacacgaagt ggctacgcaa
      541 ggcgatgcca actgccgtat gtgccatagc accgaatcct gcgaagattg ccataatggc
      601 ggtaatctga ataaaatgaa aaatagaaac gaattcagcc cgtccggtac accgtcgggt
      661 atgagtgaaa atagcagtca gattatcagt aaagcccatg cactggattt cattttcaca
      721 caccgttttg atgccaaggc taaaacttcc gaatgtcaga cctgccacga agctgagagt
      781 ttttgtgtac agtgccacaa taataatgat aaaatgtcta aaccggcttg gcataaggta
      841 gctggtttta cgtcgggtgt aggcggcggt ttgcatgctc agttagccaa aaaagacatg
      901 gaaaactgtg taagttgcca taacatcgaa gggcaggatc cgacttgtta cacgtgtcat
      961 aaagccgatg gttcacttaa aaatttctga ggacagccaa atgaaaaaga aaatattaat
     1021 aataatgtta tcccttttgg ccttcgtggg ctgcagcgaa tccgaagata ttggtaatgt
     1081 acccggccca ccggatccgc caaaaccata cagctatgta accgacataa aagctgtact
     1141 tgatgaggga tgtgcatcat gccactcggg tggtagtcca tctggcagtt ataatctatc
     1201 gacctatgcc ggagttttag gaaatggttc cgatgcaacg ccgaatgcaa tacccggaga
     1261 ttcgaccagc ctcattattt tgaagctaaa gtccggtcat caaactcccg atgcatctac
     1321 tatttctatg atatttcagt gggtttatca ggatagctgc aaagagacac tgccggacta
     1381 acaaactgtt tttttgatag agtagaatcc ccggacgatt tttcgttcgg ggattttttt
     1441 aaactttgta cagtgctaaa cacttctgtc gttcctgatc cagatcaatg ataggatgcg
     1501 gataatggct gaataacccg ctttgccgtt taatcgggtc atgtatttca cgaagcgaaa
     1561 cattttccag ctccggtacc catcgccgaa tatatacggc atccgggtca aattttttcc
     1621 cctgggtcag aggattaaaa atccgaaaat acggcgcagc atccacaccg gtaccggctg
     1681 cccattgcca gttaccgtta ttgctggata attcatagtc caacagtttt tctgcaaaat
     1741 acgcctcgcc tttgcgccaa tctatatgca gatgtttgac caaaaatgaa gccacgatca
     1801 tccgtacccg attgtgcata tatcccgttg catggagttc acgcatgccg gcatccacga
     1861 tgggataacc cgtatgccct gatttccatg cttcaaacca atcttcgcga ttagaccata
     1921 cgatgcgatc aaactggggt tgaaaactac ggattatgac gtgagggaaa tgccaaagta
     1981 tcatcataaa aaattcacgc cagatgagtt ccgacaaaaa aactttaacc gccggagcct
     2041 catgttttga tagatagtct ataagctggc gaacactgat cgtaccaaaa cgaaggtgtg
     2101 gcgcaaggtg agaggtagcg ttcagtccgg gcaagtcgcg ttgtgtatcg tatttttcga
     2161 tataatcacc ggtaaattgt gtccattgtt tttgagcggc atcggaaccg ccgtgtatga
     2221 tattttcaca ctgaacaaac ccgatctctt gcagcgaatg aataagcggt agcgacgaaa
     2281 acagaggaga aaattttttc caaggtatgg atgtactatc tgttattgca ttcggataag
     2341 aatgggtata cgtattcagc catcggtttt tataagcggt aaagacttta taaggcgtgc
     2401 cgtcatcttt tacgatttct gatttttcaa aaatgacctg atctttaaaa gagtgtacaa
     2461 ggattccttt ttggtttaat acctccgtta tcgcagtatc gcgcacgttg gcatacggct
     2521 catagtcgtg attaaagaac aatgcatcgg ttttgttttc agaaaatagc tgcggaatga
     2581 cgacttctgg ttcaccgtaa aatatatgta cggtcggcgc accatgggct ttcaatttgt
     2641 tttgaagata ggtgagcgat tcgtaaataa aactcaatcg tcggtcatcg gccggcaaac
     2701 gatccaaaat atgtttgtca aatataaaga taagtgaaac aaattggctt gccttcatag
     2761 cgtggagaag cgccgtatta tcgtctaatc gtaaatcccg ccttagccaa acaatgctgt
     2821 gctgcatgta tttatctttt gttttgaatt taactatgtt cttgcacttg ctgtacaagt
     2881 tatcgtgcaa accgaaactg acgaaccaac cgttgcaata catcaaaaac ggtctgtgat
     2941 tttgctacga gctgtgtatg gatgaccttt tgtgcatgta ttatataaaa atataattgt
     3001 ttaaaaaatc atcgcatata ttaaacaata tttcttattt tcaaaacact tttagaagtg
     3061 ttttttattg cttacactac cacgctatga tgtacaaaaa tttatccgac tgtattgccg
     3121 atttggaacg gaacggtcat ttgatacgcg ttaatgaaga agtggatccc tatcttgaga
     3181 tggcggagat ccagcgacgc gtatatcaaa aacaaggtcc ggcattatta tttacccgcg
     3241 tcaaagggtg tcgtttcccg gctgtttcca atctttttgg aacgatggac cgaacgcgct
     3301 ttatgtttcg ccatacgctc gcggctgtgc aaaaactgat cgaattaaaa gttgatccgg
     3361 cgaatctggc caaacgcccg ttgcgttatg ccggagttcc attttcagcg ttgacgatgt
     3421 tgccatggaa aaaaagcaac ggtgcggttt taaaaaatac atgccgtatc ggtgagcttc
     3481 cgcaaatcac ttgttggccc aaggatggcg ggccgtttgt cacattgccg caggtttact
     3541 ccgaacatcc ggatcacccc ggatggaaaa tgtccaatct tggcatgtac cgcatacaac
     3601 tggccggtaa tcagtatatc caggatcgtg aggtcggcgg ccattatcag attcatcgcg
     3661 gtatcggcgt gcaccatgcg gccgccattc gtcgtggtga aaagttgcgt gttagcattt
     3721 ttgtcggcgg tccgcctgcg cacaccttgg cggcggtaat gcccttaccc gaaggtttat
     3781 ccgaactgat gtttgcgggt gtactgacag gacggcgttt tcgttatggt taccaaaacg
     3841 gtcatgcggt atccatggat gctgattttg tgattacggg tactattgat acgcaagcga
     3901 caaaaccgga aggtcctttt ggagatcact taggttacta cagtctcacg cacgactatc
     3961 catacattga ggtggagcag gtgtatcacc gggatggcgc tgtatggccg tttaccgttg
     4021 tcggacgccc gccgcaagag gatacatcgt tcggggatat tattcatgaa atcacagggc
     4081 cggttatacc tacggtgatc gccggtttgc atggcgtcaa tgcggtggac ggcgcgggtg
     4141 ttcatccgct gttacttgcg atcggcagcg agcgttatgt gccttacggt gaacgtaaac
     4201 cgcaggaact tcttactatc gccaatgcca ttctcggtca ggggcaactt tctttggcaa
     4261 agtatctctt catcaccgca aaagaagacg atccttcgct tgatgtgcat gatattccta
     4321 aatttttgca acacgtactt tcacgggtgc gttgggagga ggatctgcat tttcagacac
     4381 gaacgacgat tgatacattg gattattcag gtttcggttt taatcaaggg tctaaagtgg
     4441 tgatagcatc cgcgggtaaa gtccgtcgct tattggcaag tcaggtgcct tcggaattaa
     4501 aattaccgga gggtttttcc gatgcgcacg ttattttacc gggcattgtt gccgtcagag
     4561 gagcccaata ttccgaccgc gtagccgccc gaaaagccat tcgtgtttta tgcgaatatt
     4621 tggaacatca gagagatttg gaaaacttgc cattatggat agtttgtgat gatgtgacat
     4681 ttgtcaaagc acaactcaac aattttctgt gggtgacttt tacgcgttcc gatccggcag
     4741 ctgatatgga cggagcgggt gcttttgtgg aggataaaca ttggggttgc aggggacctc
     4801 tggttattga cgcacggatc aaaccgcatc acgcaccgcc gcttatcgaa gatcccgatg
     4861 tcacgcgtcg tgtggatgca ttggctgaaa aaggaaaaag tttgtacggt attatttaag
     4921 tttcggattc attgaacggt taacttttgg agcatcatgg aaatcaattg cctgcacatt
     4981 gccgatattt ttgcacaatc cggtttggat atggtcacag aaacgctgcc caaactcatc
     5041 aaggaacgtg acattcattt tgttgtggca aacggtgaaa atacgctcga aggtaaaggt
     5101 ataaccgaag ggcaatgcca acatcttttt aatctcggcg tacatgtgat caccagcggt
     5161 aaccatattt gggatcgaaa gaaaacattt tatgcggaaa acccgttgat tcaaaaatac
     5221 ttacttcgcc cgctcaatta tcccgtgggc aatgaaggtc gtggctcgac ggtatacgaa
     5281 ttaaaaaatg gtctcaaaat cggtgtgatc aatctgcaag ggcgaaccta tatgtatgac
     5341 atagattgcc cttttcgcgt cggtgaacgc gaaattgaaa aacttcagga tgaaaccaat
     5401 atcattattg tagattttca tgcggaagcg accgctgaaa aaattgcgtt tcgtcattat
     5461 gttgatgggc gtgtgagtgc cgtgatcggg acacatacgc atgtacaaac ggcggatgaa
     5521 atgatcacct catccggtac ggcgtatatt accgatgccg gtatgacggg cccgcacgac
     5581 ggtgtgatcg gtatgcaaaa agagcccacg gtaaaaaaat ttatactcca aacgtacttc
     5641 aaatttgagc cttccaaagg ggatgtgcgc tttcacggct tgttttttaa ggttaatgcc
     5701 gaaaccgggc gtgcaaccta tgtcgaacgt gtgcgcatag acaaatcata actcatttct
     5761 tatcaagctt taacagcatt gcatccgcgg atggaacttt tttgggagcc gtgaagttta
     5821 attgataggt tcttgaaagg aacttttttc ccaaaggaga gcgcgatggc taagatttgt
     5881 gtcatagaag atgagccgaa tctaaggcta ttgtatcgtt ctgagtttga aagaatgggg
     5941 cacgaagtca tttgccacgc caatgggcgc gaagcttacc gatcacttcc ggttgatcag
     6001 cccgatctga tcatccttga tatcatgatg cctgaaggcg atggtttgga gtttctctca
     6061 aatgtactgg ttatgcatgt tggcataccg gtgattatca attcagcgta cgcgcagttt
     6121 aaaaatgatt tcgtcagttg ggcggctgag gcgtatgtcg tcaagtccag cgatttaagt
     6181 gaactgaaag agcaagtcca tcgcgttctc ttcaagtttt caatggccac cgcgtgaccc
     6241 atagatgggc acgccttaat tcacgcctta tcataaggcg cgtgagtctt actcatcccg
     6301 ctgtcgtcac agcgggattt tttattgagc ttctcttaag gtttgggtac atttagccgc
     6361 acttaacata tcacgagatt atacataata acaggagatg aatctgtgga aatcaaacgt
     6421 atcgccgtga tcggcgccgg tactatggga aacggaattg ctcacgtttt tgcgcaaacc
     6481 ggatacgatg tcaatttgat cgatatcaaa caggaatttg tcgaacgcgg taaagcgaat
     6541 atcgaaaaaa acatgggccg tcaggttacc aaaggtactt tgacggaaga acaaaaaaac
     6601 gctgcactgg tgcggatcac gctttgcacc aaaatcgaag atgcggctaa ctgtgatttc
     6661 gttgtcgaag cggcatccga aaacccggaa ataaaagcgg gcatttttaa aactctggat
     6721 aacgtatgta aacctgacgt gattttatcc accaatacgt cttcgatttc gatcacacag
     6781 atcgctgcag tgaccaaacg tgccgataaa gtgatcggga tgcattttat gaatcccgtg
     6841 cccgtcatga aactgatcga attgatccgt ggattagcaa caagccagac tacgtatgac
     6901 acggtcaaaa ctatgtccga aaaacttggc aaagtgcctg tcgaggtcaa tgattatccg
     6961 ggttttattt ccaatcgcgt gttgatgcct atgatcaatg aagcgatatt ctgtcttatg
     7021 gaaggtgtgg catccgtaga agccattgat accgtaatga aactcggcat gaatcatcct
     7081 atggggccgc tgcaacttgc cgattttatc ggtttggacg tatgtctttc catcatgaat
     7141 gtattgcatg aaggtcttgg tgacgacaaa taccgcccgt gtccgctact caaaaaaatg
     7201 gtaacagcag ggtattatgg cgtaaaaagc ggaaaaggtt tttacgacta ccccaaaaag
     7261 taatcgtaaa tttcggttga attcgtacac gcgacaatgt aacttacgtc gttgtataac
     7321 aatataaagg actgtctatg cgaattttag taatgctagt tagcctgatc gttggcgcgt
     7381 cgctcgttgc gcaacagcga accaccatca agtttggaac cgggaaaatt gatatgacct
     7441 gctccaataa agcagcggca ggattttata aaaacattga aattcgttca accgatacgc
     7501 cgattcgaat ttatttcatg aaaaacgata aaaccgttat ggacgtatcc ggttcagcgg
     7561 tttacatttc gtatgaacaa gatacacggg gcgatgatgt agccgctgtt actgtggatc
     7621 aaaagaattt ttggggtgtc aaagttattt attttgtaac ggcgacggtt cccaataaat
     7681 acgtagatat caacggtgaa caaaaggaag tggcgggaca tctcaatttg gattatttag
     7741 gattgccact ttggatttca cgaacaagca aatcttaata agataaaaat ataagattac
     7801 aaaaaaacct ccgataagga ggtttttttg ttttaccggt atagtataaa aaacggcgct
     7861 tgtgtacgcg gggaatttag cgtatgtttg tgcgttgtaa tcgtctcaat actccataaa
     7921 aatatatcta tatcaggcga tacgttatgc gattttgttt tttaatgtta tgcacattga
     7981 gtttcaccgg ttgtgacaaa gaacctgtta ccgaagatgt acagataaca cgcgggcgtg
     8041 aactgtttca cagccttcaa ttttcgtcgg acggtaaaac gtcctgcgcg acatgtcacc
     8101 ccgaagggca cacggataac cgtaactggc attttgcagc gatccacgac acgacggccg
     8161 gtattcccga ttcatttcgc accattactt tatggggcat agcacaaacc ggatcgccgt
     8221 atctttggaa aaactccttt cgttcgttgg aatccgttac gcgtttatat acggatacta
     8281 ttatgggtgg gacggcctcg gaggatgaag tggccgcttt agtggcgtat caaagatcgc
     8341 tggcatttcc aaaaaatccg aacttggcgg acaacggtca actgacagag gcgcaagcac
     8401 gcggaaagag catttacgaa gaccccaaac gcggcaactg tgcgccatgc catcctgctc
     8461 cgacctacgg aagtcctatt ccgaaaaata tttataccgg cggcacattt cgtactgtcg
     8521 gattacgtgg tctttattcg atagcacctt attttcataa tggttcggcc aaaacactgc
     8581 acgaggtgat agaacattat gccggcaatg ggcttattgc accgttgacc gcgcaggaac
     8641 gtgacgatct cgtagaatat cttaaaactt tataatatct aacgatcagt acagctatga
     8701 aacatgacaa aatgagattt gtacttattc tgagtttgat tttttcggtt atgcaatctt
     8761 gtgagaaaga aaaaatcaat gaagacgcac aaatcgctta tggaagaaaa ctttttaacg
     8821 atactaagtt ttccggcagc ggacagttaa gctgcggtac ttgtcaccct caaggtgata
     8881 tggataacga gcagtggcat ttggacctga tccatgattc acttggcggt gtggcggtat
     8941 cctattccac tccgacctta tttggcgtag cggagacggc gccctatcta tggaaaggtc
     9001 aaggtggcag cgatctggat acgttgacca aaacggttat cacgggtatt atgggcggta
     9061 cggccaccga tgaagaaata aaagccatcg gtgcatatat gcgatctttg gatgttccgg
     9121 ccaatccttt tcgaaatgat gatggttctt taactaccga gcaagcgcgc ggtaaaattg
     9181 tttttgaaaa tcagggtaag tgtaatattt gtcattccgg agagacgctc tccggtaagt
     9241 tttcgattca aatcaaagcg gcaagaccgg cttttgatgt accgtctttg cgatgggttt
     9301 ttgcaacggc gccgtactgg catgacggat cggccgggtc attgcgcgcg gtgatcaatc
     9361 attacgcaga taccatcact acggcgcaga tgacgacttg gaagtggaac ggtgccggac
     9421 aagtgacgcc caaacccata ttcgatatcg aattgacaga gcaggaaaaa aacgatttga
     9481 tcgaatatct aaaaacgttg taatcaaatt attttgagga tatggccatg agtaaatttt
     9541 gctggattgt gattgcagtg tgtttattgg cggcaccgat tcatgcacag gaagaaacgg
     9601 ttgctccgaa tacggaatcc ggaacggaag gaacatccgt aggttttggc ggttttgtgg
     9661 atgtcgcgca tgccgtcggg cgtaaagcgg ggaatgccaa tacgcttggg gataataatt
     9721 ttaacaatat gcgcacattg ttgatgttta atgcacaaca gggttcgaaa atacgcgccg
     9781 atgtagaagt tctttttgat gatcgttcta aggatcgaat tcgcttgcaa ggtgcatttg
     9841 tgacaatttt tgatgtaccc aatgaagccg tcaattttat gatcggaaaa attccgaatc
     9901 cgtttggtaa ctttgccaag cgtgaatttt ccgatgtcaa tcctttgatc ggtcaacccc
     9961 tgatgcggca ataccgcacg gcattggatt ggaataatct ctggaacaat gcggaacaga
    10021 ttgcccgaaa gagtgaacga aaaaaatatg atggcaatat accgatttcg cgatttgaag
    10081 cggcaacgcc gacggtatac gatgcgcgat gggattttgg tgtacaaatg ttcggtacga
    10141 tcggttggtt tgaataccag gcggctgtca ccgaaggttc catatccaat ccggaggcgg
    10201 atctgaataa aggcaaacaa tttgtaggtc gtatcggttt taacccggtt cccggtattc
    10261 gtatcggttt ttccggagca tacaatccgt atttgtcgaa agccgatgac cagcgattat
    10321 tggaagaagg taaaggacta ggcgagtacc atcagacggc gtacggtgcg gattgggaac
    10381 tgagctatcg ctattttatg atgagcggtg aatttgtacg cagcacttgg gatgcgacgg
    10441 tgatcgaagg cgaactgtct aactggagca tttattctga tatcaagtac aaactgcatc
    10501 caagatggta tatcgcagga cggtacgata ttatgcgttt ttccgaaatc cgtgaatcta
    10561 aattaagttc taaaaaaacg ccgtgggatt ataatgttca acgttatgaa gcgggtatcg
    10621 gatttcgtgt gacaaccgat gtgacattga agcttgtcga acagtggacg cggtttgatc
    10681 aatcggaaat tccgatgagc cgtttgacgg ctgttcaatt atccgtacca ttttaaaagc
    10741 atttacgagg attcatatga aactaatttt ttcagcgata gcgggcataa ccttttggat
    10801 attatcggtg acaggtacat ccacgcgttt atttgaagcg atggatcaaa acgcccggtt
    10861 aaatcatgac gtaatgctga tgcgtccggc gggtgatgcc aaaataacag gaacggtcag
    10921 ttttcctcga tcagctaatg ctaccgggcg tgccgtaaaa ggctcctacg gaaaaaacac
    10981 atcgcgttta aaatccggtt ccgccggcga aaaaagagcg gtggtatata ttgcctcggc
    11041 caagggtaat ttcccggcgc cgcaaagccg acctgctatg cgtcaaaaaa atatagccat
    11101 tacaccacgt attttaccgg tgctggcggg aacgacagtg gacttcccca atgaagatga
    11161 tttgtttcac aatatttttt cactttcgcc ttccgtcaaa tttgatctcg ggcgttatgc
    11221 caaaggtgta tccaagtctg tcaaatttac tacaccgggt gttgtcaaag tgttttgtga
    11281 catccattcg actatgggcg gagtcgttct ggttttacaa aacccgtatt ataccgtgac
    11341 ggaagccgat ggaagttatg cattatccgg tgtaccggcg gggacgtatg atctggtggc
    11401 atggcatgaa gggggtacgc aaactcaaaa aattacgata tcagcttccg gagtgatcac
    11461 acaaaatttt gctttttgag aagtcatacc attgttaagt gaaaaaaaac ctgctttcgg
    11521 caggtttttc tatttatacc caagcctgat aggccataaa ggcatttatt ttttgcttta
    11581 tcatatcttg ttttcagttc attaaactcc gtgatgatcc gtcgaaccga tatgggttta
    11641 tcgcttgcaa gaaatcgggt gagtgtgtac attaggccgc tttttcagaa aagttccgtg
    11701 cggttttgca ggcacgacac atagcgatta aagcctgtga tttcaagata gaggatttct
    11761 gaatcaggaa attgaatgaa gattaaccgt ccatcattcg tccgagccat ttgataagcc
    11821 ttgatcaacg tattgattcc gactgaaccg atgtgttgca tgtcaaattc aaggatcaaa
    11881 tccgtttggc cggtttcttt gcggatcaga tcgtaggcat tttggagaac gaccaaatca
    11941 tcgtctgaat cgtacggatt gaatcggcct tcgaggacta gaagcacatg ggttttaaaa
    12001 atttcgtgtg tcgcgcgtag ggatggcata tagggtttat ttgtaatttc aacagagata
    12061 ctacacacaa acttattttc aaacaatata aaaggattaa gccgatatga agtcaaatag
    12121 gtattggttc gttgcgttgg ttttgattgg ttttatctca tcatgccaat cgtcgaaaac
    12181 ggagacggcg gctgatttat ttaaacaggc ggacagcctt tttacaaaaa acaaaacgga
    12241 tgaagcgttt aaagtgttgg ccgagatgga aacgcgtttt aaatccgata cgactgtgat
    12301 gtgtgaagtg tggcgtgtaa cggctgaagc gtatgcgcag tataaaaatg aatttagcaa
    12361 aaccatagaa tattatgacc gtattgtgaa tacctatccc aatacgcccc aagccgtggc
    12421 cagtttattc aaaaaagcat acacctatga aaacgggttg aatgataatg atcgcgcaaa
    12481 agcggcgtat gaagaattta tcaaaaaata tcccaatcat gaactagccg atgatgcgca
    12541 aacgatgatc gattttctcg gtaaaccggc ggatgatttg atcgaaatgc tggagaaaaa
    12601 ggctaaacaa aatgaaaaaa aatcaacacc tgctaaataa cttaatcgga gtctatgata
    12661 aatttatttg aaacccgcgc taaaattatt tgtacgattg gcccgtcatc gagcgattac
    12721 gaaactttgg taaggatgat tgatgccggt atggatattg cgcgtcttaa tttttcgcac
    12781 ggctcacatg acgcgcatcg ccaggtaatg gacaatattc gtaaagcggc ggcgcaggtt
    12841 ggtaagcagg tagcgcttct tcaggattta cagggtccta aaatacgtgt gggaattttt
    12901 aaaaatggcc ccattgaatt gcacatcggt gaaaaattta caattacatc cagggatgta
    12961 gatggtgatc agcatatcgt ttccaccacg tacaaggagc tgcccgggga cgtaaaagtc
    13021 ggtgatgatt tacttttaga tgacggatta ctcaggcttc gtgttgtttc taaaaccggt
    13081 accgatgtgg tgtgtgaagt catcatcggc ggcgtgttga aaaataacaa aggaattaat
    13141 ttgcccggtg tacatgtcag tgcaccttcg ttgacggaaa aagatgcgga ggatctactt
    13201 ttcggtttgg ctaatgatgt agattatgtc gccctttcgt ttgttcgcaa accggaagat
    13261 atactgcatg ttaaagaagt gatacgcgct cacggcaaga atacaccggt cgtggcaaaa
    13321 atcgaaaaac cggaagctat tgattgtata gaacgcatta tagcgataac ggacggattg
    13381 atggttgcac gcggtgatct tggcgttgag atgaaaaccg aagaagttcc gcccattcag
    13441 aagcgattga ttcatctttg caaccgtgcc ggagtgccgg tgatcacggc aacacaaatg
    13501 cttgattcga tggttaacaa tccgcgtccg acgcgtgcgg aggcttcgga cgttgccaat
    13561 gccatattgg acggttcgga cgccgtgatg ttatcggcgg aaacagcatc gggtaaatat
    13621 ccggtggaaa ccatcgccac catgaaacaa atcattaaac taatcgaaaa agaaattccc
    13681 cacgatcaga gcaaacgtcg caagacaggc gtagaattgc aaatgatgca ggaggggata
    13741 gccatcgccg cttgcgggct tgccgatatg ctagaagccg atgccatcgt taatattacg
    13801 atgacaggcg ctatgtctaa gctcgtggct aaacaccgtc ccggtacacc catcattgca
    13861 attacacatt cggagcaggt atgtcgtcaa cttaatcttg tgtggggcgt acgcggtatc
    13921 aatgttgctg atctgaaaga ggatattgat gaaagtattg aaaaagttaa aaaagctctt
    13981 gtaaaacgta ggattctatc tgcaggccag cgtatcctaa taaccgcagg tttgcctttt
    14041 ggcggacgcg gtgcgaccaa tacggttcgg gttgaaacgg tttagtctgt aaaaccgcat
    14101 caacgcacga cagcgatttt accggttttg gaataatcgc caatattcaa acgatagata
    14161 taaatgccgg agggtaaaaa ctttccggca ttatctttta tatcccaatt taaaagatga
    14221 ttattaggat caaatttgcc gtagttatga tgtaccatcc gttttccgga aattgaataa
    14281 acctcgaaaa ctacagatgc cgtttttcgc agatcaaatt tgatgttgag cgtgttgtga
    14341 aggtccattc ggatcggatt ggggtagatt tgcagattag taaccgttgc atgtgaagaa
    14401 gagccatctg ttaaaccggt actgatcgta taggtcgtat tggtcttatt tgtagtcgtg
    14461 ttgctgacga taacgatcaa agaatcaccc gctttcatat gcgatacaac aaaactgtca
    14521 acatggctcc gacgggttat aaaaggatcg ccggattcca aacgttcaat ggtaataata
    14581 ttaaaatcgg aagggctacc tgattcgaaa cgaattttaa tatcactgga gtcaaaggtt
    14641 ttaaagttat aataccgtgc cgccaaaccg ggtatgccat cggtataagt taaattaccg
    14701 atcaaagtat ttttataaaa acttaccgca ggaaacgagc gactttcctg aaaatatgta
    14761 atactgtcat aacgatcttt tgtaaaatag ttccagattg aaaattcggt aaatgaattt
    14821 ctaaggtcag tacttgtttc ggcttcgaga gcattattta tagcgatcaa cgcccgttcg
    14881 tcgggcatgc gttcccatat atttcgaact atagaagcgc cgtgggtctt ttccatcata
    14941 tgcaaccaat gtgtaacatg gtaaccaaaa aacgtcgtct cgctgaataa agacacgtgg
    15001 gggttgctgt aaaatcttgg tagataagcg taatagtcgt tgacctcgtc atatactaca
    15061 tcttccagcc aagttgaagt catttcataa aaccaaccgt ccgtataacc ggtatcgtat
    15121 ttataggcca attgaatggc gtggtgaaat tcgtgcgccg ccgttacccg catggcgtca
    15181 taaccgtgcg ttttgaatcc accgcctaca aaatcatttt caatttcaat gaaggattcc
    15241 catgtatcgc ttccggatgg atattgctgc accggtgccg tctgaccgta agctctgata
    15301 ttgataatgt atacctcata atatccgtca cgtttcatat tgacgggttc acgatatccc
    15361 atggtatcaa tttctttacg gtatgatttt tcgaaggcct ttccgttttc ttcaacccag
    15421 tcgggaatgg tattggcatc ggcatcaagg gttgatactt tatctgtgcc taccgtatcg
    15481 tagtatattt taaacgtacg attttttcct gaaccggtta caaacgcgtg attatataca
    15541 aaagcggctg attttttctg aacagtaaat gatttgttta atttttgaga aagggtttgt
    15601 aaagtttgaa tcgccggaaa accgcatttg tcggcctcat ggatgggtgt agaatcagat
    15661 gccgtaagca aatcaaacaa tgtatcgtag gcttgttgat cactgataag accatcggca
    15721 tggtctttac acgtttgcga aaggtgtcct tgggctagtg cataaccggt tgtagtaagg
    15781 actataaata aaaatagacg gtttatcatt cgtaacgatc cctgcttttt tttctaaaag
    15841 ttagtgacaa agggacgcct tcaaaaccaa actcatcccg catttgattt tcgatgtact
    15901 ggcgatagtt agccggaatg agggttggtt cattggagaa aatcgcgaaa accggtggcc
    15961 gtgatttgat ttgcgtcgcg tagttcagtt tgatttcttt accgcgtacg gcgggtgggt
    16021 gattgcgcgc aatgatgcgt tcgagaaagc ggttgagttg cgctgttgca atagtccggt
    16081 tacgctcgtc atgtactttt ttagcggttt cgaggacttt ggcaacgcgc tgtttggtaa
    16141 gcgccgatgt aaaaacatac ggaatatatt tggttgcgcc gagcgctttg tccaaatgct
    16201 tttcgtaatc aagcgccgtt ttgtcggttt ttttaacaag atcccattta tttacaacca
    16261 aaattaatcc ttttttcagt tgccgggctt cttcaagaac gcgaatgtcc tgagccgcga
    16321 gcccatcaat cgcatcaatc aagagaatcg ctacatcgca ctcttgtatt gttttgagtg
    16381 tacgaagggt gctgtaaaat tcgagatttt cttttacttt ggctttgcgt cgcagaccgg
    16441 ccgtatcaat caatttgaat gcggtatcat tgtaaataaa atcagtatca atcgcgtcgc
    16501 gtgttgttcc cggtatatcc gaaacgatat gtttattttt accgatgatg gcgttgacca
    16561 aagaagattt tccgacattg gggcgtccga taatagccaa acgaatagtg ttagtatcgg
    16621 tcgttatttc gtccgggttg gccgcaaaac ggtcaccaag cgccgtcatg atacggtcga
    16681 gcatgtcgcc gatatggcga ctgttcagcg ctgataccat cagcggttgc cccatggcca
    16741 gtttataaat ttcctgatcc gccagtgtat tttgataaac tttttcgttg tccactttat
    16801 tgactatcaa taacgatgtc tttttagaac gttttaaaac atcggcaatt ttcatttctg
    16861 tcgtggaaag ttggcttacg gcatcaatca gtaaaataac gagatcggat tcgtcaatag
    16921 ccagctgcgc ttgctcacgg atagcggtat tgatgagatc atgcgaatcc ggaataaacc
    16981 cgcccgtatc gatgagaata aaattttttc cacgccattc ggcgatttca tagtgacggt
    17041 cccgtgttac tccgggtgta ccgtctacga tggcatcctg acgtccgatg atgcggttga
    17101 atagcgttga ttttcctaca ttgggccgtc cgactatcgt gacgacggga aggcggttga
    17161 ttttttttga ggccacttta aaagtcaata ttgaatatat atacttgatt taataaccta
    17221 accaacagcg cgtactattt caaggtttaa ataggaattt atgtatatac gatcgcttca
    17281 aatacgcgat tttatgggtt tttcgttgta tcggaccgaa ttcaaagagg ggttttctgc
    17341 tttgatgatg gggccgtccc aaggaaaaac cggttttcga atggccatgc gcgttgtact
    17401 cacggatcat tttgatcgca attttgcctt gtttcaccgc aatcattccg gcacgtctga
    17461 aatcaattta gagctgtatt gcggaaagag tgccttaaga tttaataaga cttggaccga
    17521 cggtgccgta caacaatgga ctgtagaaaa tccggatgcc ggcacggttt tagaccttac
    17581 aaaccaggga acgtatgatg actgggtgcg atggttcttg tatgaaacgc aaggttggcc
    17641 ggtgagcgaa catgatggtg agaccgtttt gcacaatgaa tgggcgcgtg ggcagtacga
    17701 ttttttgatg cgccttattt tttgtgatga tacgactatg actgatctca tgacgatacc
    17761 gatttcttct ttggttggcc ttcggtatgt acagaacatg gaagtacgat taaacgaatt
    17821 gatacaaaaa gcgtcggaaa aagtatttat tctcaaatcg gaagaaaatc tcgtccaaaa
    17881 cgaactgtca ttattagaac aaaagcgaca aatggtttta gtgttggaaa aggatttgga
    17941 aagcgatacc gaattacttc aaaaattgga cgaaagactg attcaaacgc aacacgaacg
    18001 cgtacaagcc gagcgcatca ccggctatat tcaggaaaag cgcagcgaaa ttaccaaact
    18061 aggttttgaa attacgacgt atcaaaacgt catgcaaaac aacgtactgc aagatacgtc
    18121 ggaagaagaa atacgtacga tggatcgtaa agcacggtta tatgaaaaag ccatcaaaga
    18181 aatagaaacc atcgaagaaa acatcataca acgacggcac ttagaaaaaa atctcgaggg
    18241 tatcgagcag gagatccagg tcatagatcg cacgcatacg ggcaaattac ctccggaaat
    18301 aaaaacgcaa aaaaaagcgt acgagtccga agcgcgtcgt ttacgcttgt tgctgtccaa
    18361 aatgcaggat gatccttctt cgatggagca ttggcaaact gaaaaacgcg cgcatgaaga
    18421 agcgtatcat cagtatacga ttctcaaaaa aacgcgtcag tacatgaacc aaaataacct
    18481 gcaaaagctg aaatcaacga ctgataaatt gatcgtgaag cgccaacaca tgcaggatga
    18541 actggatcag atatttgcat cccagcacga aactcaatat cagaaactgg aagcggccgt
    18601 tgcatcggat cgccagcaag cgctcgaact gaaagagcgg atggctacgg ccgttggaag
    18661 aaaagagaaa ttacagcagg atatacaagc gcttcaactt gtcgtggata agcacctttc
    18721 gcgggccgat cagaaacgtc gagaaatcac atcaaaaagg catttgcttt ttgttcgcga
    18781 gacggtaacg gatttttata acgcgcaaat cgagcttcga aaaaacgaac tgagccgcca
    18841 aattaaaacg catttaaaga aatttagagt tgcgtttacg agtaaacaac tgttcggaaa
    18901 aaacaaaaca aacgaacctt cgcttgaaac gataccgata tccacatcag agcggattct
    18961 ttctgagcat atttttgcca tgacagttct ggctatgaat ttaaaaaatc ctattttttt
    19021 tgctgatgag aacttttcgt ggtatctgac gaaagatgat cgtacggaga tcgaagcatt
    19081 ggccgatcaa ttaaattttg gtcaattcat tttgttcgaa aaaaattaaa attctatatc
    19141 gcccacaatg accgattcgc gtatcggcgc gacgagttcc ttaacgaccc tcagatgttc
    19201 aggatgatcg gcataaattc gcaaatcttc cttattttta aaacgacttg taagcataat
    19261 atcgtaccct tggcggcgct cggaaaaatt aggccttgcg tgaatttcaa tgacttgcgg
    19321 tattcgatgt ttcagatctt ccaaagcagt gcacatgttt tcgatagcag tagtatctgc
    19381 acccggcttt agtttgaata aaacgatgtg atcaatcaag gtatcctcct tgtttaaaaa
    19441 gcaaattcta atgcgcgttt ggcggtaaaa taacggttat agatgtaggt tgatttggcc
    19501 tcaatctcta ttttatatat tcctgaaggc gctaattgtt cggtcatagc gggttgtttg
    19561 ccttgtgtag aatcggatct gttttgttcg cgaaattgta aaatgtcccg caacgggctg
    19621 ttggatttat aagctctgtt ccactgtagc attttgattc ccggcgatgt catgctatta
    19681 cgggcaagcg tagcgacaag ttttcctttt tcatccacga tgcgagctgt cagacgggat
    19741 gtctccggta aataaaatga aaatactaac tggctatcct tgatcgcgac attgagattc
    19801 tgtatgtctg ttccgatatg ataatactgt gcgctgatct tatcggcaat aaatacctga
    19861 ccaaattttt ttccaatagc tatggcacga ggttctataa attgattttc accgtcaccg
    19921 ggcgaaccga aagtgcccat ataacgaaga tcgcgatcaa acttgtgaat ggcatgattt
    19981 tctgcgtcgg taacccaaac attattaaaa ccgtcaatag ctaccgatgt cagattgact
    20041 ttgttgaagc catattctga tgaaaccaca cccatttgaa aatgcccatc gaatcctaat
    20101 ttttgaatgc gtgtgccatc catgtcaacg acgataagaa agtggtcttg tgtgaaatta
    20161 tccgggtcac tgccgtctga cgcggcaatg gcatcgggac gatacagtga caatgtaccg
    20221 gaagcgtcgg tcgcattgcc ccaaatctgg cacgggatga ggttggaatc taatacctga
    20281 atgcgatcat ttttatagtc ggtcacatag acttgatttt tttgatctac ggctacgccg
    20341 cgcggttcat catattcgcc ggatttattt cctttttttc ctgaagcttt gacgaaaagc
    20401 aagtatcctc cgggattctt tagtttgacg agacgattat taccggtatc ggctatataa
    20461 acatcaccat cactattgca agcgatgcca cgcggacgtt tgagtttttg ctgagggcct
    20521 ttaccttcat acacgtctaa gccctgcata gagctgttga agatgatatt attctgaccg
    20581 gaattgacac cataagccgt gagatcatcg tcgtcattgc gattatcggg atcgtcggta
    20641 gatttcaatc gggtaacggc caaaccgcgg ggatccgaag gcgtgatgtc ggagcgtcct
    20701 atcaatgcca taaatgcaaa taaatgaact gccgtggctt tgtgaatcgg tgcgtgatta
    20761 gcgggcggat ataccagaag agtttctgat tttcgaatgc gaggttttgt gttttccgca
    20821 agatcttgag atcggccaaa cgaaataaca aagaaaaaca gggttattaa aaagtaatct
    20881 ttcatcattg cccctcgatg gcttgtaacg ccgattcgat ggcataccgt acatcggaat
    20941 tattagtctt gccgaggctt tgttgcaaaa tggtttttgc agaacgtgca tccggaccga
    21001 tcttaccaag tatttcagcg atgatgtaca taagtccgcc gtcctgatcg gttaatgatt
    21061 gcaatttgga tatgagcttg ggtacggccg atgtgcctat ttgtataagc gctgttttag
    21121 cattatccga aaaccggtaa tcgtaactca taagcgaagc catcataaaa tcaattgccg
    21181 caggggccga agttttcaaa tgccctaagg tgattattgc cgatgattgc atttctgtca
    21241 gtgtttcttt gtgctccaaa tttcgcttgg agaatatcgc gataagttgc gactctaaag
    21301 gcttaggctt cattcccatt ttttcaatca tcaacaatgt ttgcaactgc tcatcccaat
    21361 tatctttacc gctaagtatt attttggctt cgtccatggt aggtatgatg ccgggtacgg
    21421 caatgtcata ccgcaaacag aaatcaatgc gttcgtcttt ttgacttttg taaggagttt
    21481 ttggtgcatt ggcggcaatg cgttcggacg ttttatttac aaagtatttc aaatccgatt
    21541 cgggaaatgc agcggttatt tctttgtttc gctctgcatt cgtggtcagg cgaaattgac
    21601 ggatgaaatc aaaaaggcga tcggctatat cgtccgaggc atcgaaacga tggaaatatt
    21661 cgctgagcca atccaatact tggcgtttat tttcagatgt aggcaaagtt tgatacagac
    21721 ggttgagcag ggcatagata tctttttgtg cttgcgagtc ccactgaatg tgtttctgat
    21781 aatttttgta agcataagac attagagtcg gtgtatcttt aaaaccgtcg gctaaatgat
    21841 ccaacgcatg attaaacgaa atgggctttg gtaaacctat tttattgtcg agcgcccgat
    21901 tcaaataggc atcgacacgt ggtgaaaatc taagatcatc ggtaggaaag agaaggttga
    21961 gacaaatgta taaacgataa tttctcatcc gtgttatcgt ttctaatccg atacgccatt
    22021 cttcttcgtc aatcacgctg tctgctgcaa aatagcgtat ggtttctttg gagcgatcat
    22081 cctgatcggg attggcgatg ccccccaacg tttgtatcaa aaattttttg taggccgtgt
    22141 tttgaatttt ataccgcgtg aagacataca atacatcata atgcacgtta ccgcattcgg
    22201 tgtcaaaagg tattcgtacg gcttcatcaa cggcggactg tattttggat gacgtggtaa
    22261 gatcattgag cttatgtttt aacaaatccg cattttgttt gcatcgttca gcttcggtta
    22321 tcgccctgga tgcggcgttt acattgtgtt cgcctggaaa taacgaggcc agatcagccg
    22381 tcatggcgat gctacccgaa aaactaaaat agatctcgcc ggtaatgacg tcaatcaatc
    22441 ggcaattaac atctatgtag tttttaagtt tggtatatgt gcctgaaaat aaaacgtcta
    22501 tcggaataag ttcgccgact ttttgcgcct gttcgctatt catcaggtcg ctcatcatca
    22561 aagcattttc ttttaaaacg acgtccaatc gccggcgctc aaaaagtcga aattgtttgg
    22621 tgcggcctaa agccgatatg agcgattcgg tgatatattc accaaattca tttttatttt
    22681 cagcatcagt tttggtcggt acaaaagtaa ctacagctaa acgctgcggt ttaccggttg
    22741 tattagagga ctgtattttg gcggcaagct ccttgatgac tttgccgtat tcatccgagg
    22801 taatagccca aattccgggc gcacagagcc acgtgagtgc ccacaagtaa taaatgtttt
    22861 tcatgcaatt ttttgttaat aggtacgcta tgatataaat caatattgga tcagcgcaaa
    22921 atatcggtat tagaaactat ttgcaagttc atgtagaaaa aagtagtttt acaggaataa
    22981 ttgacatttc aatacgttct attttttggc agctcatgac gtctaaggat tttgaaaagc
    23041 aagcactcgc acatacggaa tcgctgtata atacggcgct acgtatgacg cacaatccgc
    23101 aggatgctga agatttggtg caagaggcat tttacaaggc ttttcgttcc atacatcagt
    23161 ttgaagaagg taccaatctt cgcgcttggc tttttaagat tttagtcaat acgcatatat
    23221 cgcaataccg taaaaatgca aaagaccctg cgacgacggg ttacgatgat gtcgaagagt
    23281 tttcattgta tagccaagta agaagccgcg ctgcttttcc ggatgatgcg ccgacggata
    23341 ttttaaatca gtttttagat aaagatatcc aggcggcgat acaggcgtta cctcagcaat
    23401 ttaaagatgt ggtaatgctg gttgagatcg aaggtttttc gtatcaggaa gcggcgaata
    23461 ttttagatgt acctgtcggt acgattatgt cacgattatt cagagggcgt aagctgctgc
    23521 aaaaatctct atgggaatac gcagtggaat acggatatgt gaagaaataa atagtaaagc
    23581 ggagttatga atttcggagg agtggaggag aggttatgag tcatgtacat catggcgagg
    23641 cgggtctgca tgcgcagaac catccgatca attgcggtga aattgaacga cttttgtatg
    23701 cttatttgga tggtgagttg ccattggaag aagttgcgcc ttacaaagat catctatcgc
    23761 attgcccgcc ttgcaaagca tttgtcgaat ttgaagaaaa aatcagccat ctcatcaagc
    23821 aaaaatgcgg tcacaacggt acagatcgcg cttgtgtgcc gacatcgctt catgaaaaaa
    23881 tacaaaaggc gattgcattg tccaaagaag tttgatgtgt gatagactga taataagaga
    23941 taaaatataa cggctaatgt taatactact ttaccgccaa ccttgagcaa taggtaatcg
    24001 cctcccaaaa ccgaacgact tagaagttac gcgcaaaccc ggtgcagcct gcctgcgttt
    24061 atattcattt cgatcaataa gtttgagaat tttgtgaacc gtctccgggt cgtggccaag
    24121 ctttataatc tcatcggcct ctaaaagatc ttcgatatag gctttcagaa tggcatccag
    24181 tacgtcataa ggcggaagcg aatcctgatc ggtttgattg gggcgcaatt cggcagaagg
    24241 cggtttttcg atggtacttt tgggaataat tattttatcg cgattgatcc aatttgaaat
    24301 gcgataaacc atcgtcttgg gcacatcact gatcacggct aaaccgccgc acatatcgcc
    24361 atagatcgtg cagtatccga cagccaattc ggatttgttg ccggtgctaa gtaccatggc
    24421 gttcaattta tttgaaaacg ccatcaagat gttgccgcga attcgggctt ggatattttc
    24481 ttccgtcgta tcctcgggca aaccttggaa gctaccggat aaactctcca agaaactgcg
    24541 gaacatgggt tcgataggaa cgctatggta cgtgatgtcg agattccggg ccaggtcgcg
    24601 agcgtcatct tggctgtgat cggaggaata tcgggacggc atggatacgc cgatcacatt
    24661 ctgcgggccc aaggcactcg cggtgatcgc agcaaccaac gccgaatcaa taccgccgga
    24721 caatccgatc aaggctttgc ggaatccgca tttgtggagg taatcccgta cgccgagcac
    24781 gagggcatta aaaatttctt cttcggttgt cggattgggc atagtgatcg tttgcgttga
    24841 aggaagggtg agagaaaccc gatcttcctc aaaggccttt ccggtcgcga ttggtattcc
    24901 ttccgcattg agcgcaaaac tacgcccgtc aaatatcaag ctgtcattgc cgccgacaag
    24961 attgacgaaa agtatcggtt tacggtattt aacagcgatg ctgcgaaaca tttccaaacg
    25021 caaatgatgt ttttgtacat ggaaaggtga cgcggaaata ttgatcagta tttctgcacc
    25081 ttgattgaaa agttcttcga tcgggtcatt gtgataaata cgtcgcttcc aaaaatcctt
    25141 gtcattccaa ctgtcttcgc aaatggagat gccgagtttg acaccgcgaa aagtaaatat
    25201 ttttttatgt tttgccggtt caaaatgacg catttcgtca aatacatcat agttgggcag
    25261 tagcgttttg tattgtatgc cgataatctg gcggttttcg atcagagccg ctgcgttaaa
    25321 tacatgatga ccttccggat cattattggg cgcgacaaag ccgcacaaaa cgccgacgcc
    25381 gtcggtggct tccctgatcc gatccaacat ctcctgattt tttctgacaa aagctttttt
    25441 ctcaacgaga tcctccggag gataacccgt aatgctcaat tcaggaaata cgatcagatc
    25501 agcgccttcg gagcgcgcct gattgatgtg ttggataatc cgttccgtat tattggtgag
    25561 atcgccgacg cgggaattga tttgagctaa aatgactttc atgtattaac tatgatcgtt
    25621 taggcttatc aataaaaaag gtcactcgaa aatgcgtcca gcttgtctga aaaatgagaa
    25681 tctgcgtggg aaatataaaa tcggcacaac agatgcgtga ttcagtatgc tatagatgac
    25741 gctttttgga gcaacctttg tgtgaatttc ttaatcggtt atcaatgccg taaaacttcg
    25801 agaactttgc cgccgacacg aaaagattga tattgcggat caacttcgat cggcgaaagt
    25861 tcggcattgg ccggttgtaa aataatttta cttccgcgtt tgtaaatacg ttttaccgta
    25921 gcttcttcat tgacgtaggc aataacgatg tcaccattga caaattcgtt agtctgacgg
    25981 catacgatgt agtttccgtt aaaaataccg tcttcgatca tcgagtctcc aacaatctgg
    26041 agtgcaaaaa gttttttatc ctccggcgaa taacgggaag aaacaacatg gccgagcgaa
    26101 atctcttcac taaagcgcgg tgttccggca gccactttac cgacgacggg aatgcccgga
    26161 ttttgctgat tatcgtaaat aatcttcggt tccatattta tactcccaaa taatggagaa
    26221 ataaacgatg attggattct ttgatttgtt caagccgcat tagaaatgcg tcgttgtttt
    26281 taaagtcgag aaaattggcg ataaaggcaa gctgtttttc atcggaaggc aaacgcgaag
    26341 acttgcgttc gaatagtaaa aaagccgctt cttcgatgcg tcgtaaaaga taatagttgg
    26401 ttgtaagtat agacgcatcg tcaaccagaa gcaggcctgc cgatatcagg ctgttgatgg
    26461 cttgtacggc gctttgcaac ggtgattgcg gcaaacgatc gccgtggagc aattgtaatc
    26521 cttgcacgag gtattcgatg tccaacaatc cgccgcggca tgttttgatt tcatacaccg
    26581 catcggccgg agatttgatt ttttcgcgta tttgacgttc acgcatagtg gttatttcat
    26641 ggcgaatatt ccattttctt gaggccgtcc ttagttgctc ataacgccac gattcgaatt
    26701 gagagtgaag atcggacgaa ccggcaatac agcgcgcctt gatcagcgac tggacctccc
    26761 aaaacaaggc acggcttgaa aaatattcct tatatcgcga cagcgtaacg acaagtggcg
    26821 cttgggcgcc ttccgggcgc aaacgggcat ccatatcata aattttcccc tcggacgcgt
    26881 ggtcggtgat gcgctgatga acgcggcgtg cttgagaaat aaaatgatct acatccgcgt
    26941 catccgaaca cacccaaacg acatccaaat cggatcggta gctcagttca cttccgccca
    27001 actttcccat acctaagaga ataattggtt ctttcatcgc gaacacttca ctgaaggcgg
    27061 tttgtaaaat ttggtctgcg agctccgaaa gatataaata gggctctgac gacgaaatcg
    27121 gttggttgcg atttgtgagg gcgcattgaa cccacccaag acggcgaatg tgctgaagat
    27181 tttttgtcaa tcggtaaatg gcttgtgcgc cctgatgagg cgttgataac catgtcggga
    27241 gctgtatctg cagccaatcc aaataggaag gtctttcaca aaaaatctgt gtcaatcccg
    27301 gcgctaacga acagagataa acaaggtgtt ttcgatattc ggcattatgc gacaacgagc
    27361 gatatagcat atcggcaaaa ggttgcgccg cgacgatgcg ttcaagatgt gccagcgtca
    27421 aatccggatc gtgcgtctga ccgacatcag tcagtaagat ttccagcagt tccgcaaggc
    27481 attcacgaac tgagttgccg tataatttgg gaaactgtcc catagccaaa aggcgaatcg
    27541 tacgatgcgt tcgatccgga tcggtgaaat gatgttcctg caaaaaacga attccttctt
    27601 ccgaacatac ctctgaaatt aatcccgaaa atactgaggt tgcacggtta tcaatttcaa
    27661 atacagatag ataaatcgcg cgaacagatt tcaaattttc atttaaaact gattctaatg
    27721 tcgaaccgtt cgtatagcct agtagaatcg cgattttggt gaattcatcg gttgtcccgc
    27781 tcagggtgtg cttctgggtg tctttttcga tctgaagtat gtgctcgatt tttcgataaa
    27841 aaatatacgc ggagctcagc gtttgaaatt cgttgggcga aagcagtttt tgtgaaaaga
    27901 gctgtttgag tcctttgagt gtgtttccgg ttcgaatttc cgtcatttgt ccgccgtgga
    27961 taagctgaag cgcttgtacg ataaattcga tatcgcggat tccgccgggg catattttga
    28021 tgttaaggtg atcggaggat ttttgttctt cgatgcgcca cttcatggcg gcgacttcgt
    28081 gcaagggcga ttggaaaaaa gttttaggat acacaaaagg ttccaaccgt ttgataaaac
    28141 gagcgccgaa ttccaaatcg ccggccacgg gtctggcctt gataagcatt tgccgctccc
    28201 acgtttgacc gcgcgcttcg taataataca aaaaactgga aacacaccgc gcaagcggac
    28261 cggcatcgcc gtcaggacgg agtcgcgcat ccacacggta aagctgaccc tcggatgtat
    28321 ttttagcaat gtgtgcgatc aggccggacg ctaagcgatt aaaatattca tgatgggtca
    28381 agcgatgctc gttagcgacg tattcgtctt cttcttcata cacaaacatc acatcaatat
    28441 cggaactgta attcagctcg cccccgccaa attttccaag agcgattaca caaaacggtg
    28501 tctccggatt tccccaacgt gccgccatat cccgacgctg ggcatccaaa gcgccttgca
    28561 gtacacattc ggctaagtcg ctcacggatg cgacggtttt ttcgagcgga tcctgtagta
    28621 tataatcccg aatacccagt ttcagcattt cacggcgttt aattttccgc aatgcatcca
    28681 tgactttgtt gagcgacggc gtttgattat gaaaaaggcg gagaatatgc tgctggacct
    28741 caagtttgtc aaaaggacgg caatcgtact cggggcgaac gagaaaataa gcgtattgga
    28801 aatcacggat cattagttcc gcgataaatg ggctggatgc aaagagccgc accagacgtg
    28861 caattgtagg cggcgtatca ctgagcattt taaaaaaagc gatgcgatca atcaccgcac
    28921 ggctaaggcg ttcaaataaa ttgaatatat cgtcgcaatt cggatatggg cgcaattcgt
    28981 ccaatagccg tttgagaaag cggatatcat gcggttgggt ttggtttgta atgcttttca
    29041 gattggtttc ggcctgtgct gtattatgaa aaaaactcag gtcatgtgaa aaagattcaa
    29101 gcatgtttta gtttgaaaaa gaaaagacga ttccgtaagt tttaatgtct atgaatttac
    29161 ttctaaaaat aacaattcca atcataaata ctccggagcc tctatatgtc tgataaacgg
    29221 gtagaagaac tccatcgcct taaagcagag gccttgctgg gtggtggaca gaagcgcatt
    29281 gatgcgcaac ataaaaaagg taaacttacg gcacgggaac gtatcgctgt tcttttagat
    29341 gaaggtagtt ttgaagaatt ggatatgctc gcacgtcatc gttcgcacga ctttggtttg
    29401 gataaggaaa gaccgctcgg tgacggcgta gtcacggggt atggtactat tgacggccgt
    29461 ttggtgtatg ttttcagtca ggatttcacg gtattcggcg gcagtttgtc ggaagtgcat
    29521 gcggaaaaaa tcataaaaat tatgaatctc gccatgaaag tcggagcgcc ggtgatcggt
    29581 cttaacgatt cgggcggtgc gcgaattcag gagggcgtcg taagtctcgg cggttacgcg
    29641 gatatttttc ttttgaatac actggcctca ggagtcgtgc ctcaaatttc ggcgattatg
    29701 ggaccgtgtg ccggtggcgc ggtatattct ccggcgatta ccgattttat tctgatggtc
    29761 aaaaatacca gctatatgtt tgtcaccgga cccaatgtgg tcaaaacggt tacgcatgaa
    29821 gatgtgagtt ctgaagatct cggcggcgct atgacccatg caagcaaaag cggtgttgca
    29881 cacgttgcgg cggaaaacga attggactgt ctgatgaaaa tcagaaaatt gtacagctat
    29941 atgccatcca ataatatgga agatccgccg gtgataccct gcgatgatcc ggccgatcgt
    30001 tgtgaagagg cgctcaaaac catcattccg gacaacccta ataaaccgta cgatatcaaa
    30061 gacgtaatca aactcatcgt ggataacggt gatttttttg aaatacacga agaatacgcc
    30121 ggcaatatcg ttgtcggatt tgcacgactc ggagggcgtt ccgtaggtat cgtcgccaac
    30181 caaccggcgg tactcgcagg tgtgctggac attgactctt cgatcaaagg cgcacggttt
    30241 gttcgttttt gtgattgttt caatataccg cttgttgttt ttgaagatgt ccccggattc
    30301 ttgccgggta cggatcagga atggcgcggc attatcaaac atggcgctaa attgctctat
    30361 gcattttgtg aagcgacggt gcccaaagtc acggtcatca cgcgcaaagc ttatggcggt
    30421 gcgtacgacg tgatgaattc caaacacgtg cgtggcgata tgaactttgc ctggccgtcg
    30481 gccgaaatcg ccgtgatggg cgctaaagga gcggtggaga tcatctttaa aaaagaaata
    30541 gacgcctcgg ccaacccgaa agagaccgaa gagaaactga cggaagaata caaagaaaaa
    30601 tttgccaacc cttatagtgc cgccgaacgc ggatatgtgg acgatgtcat tgagccgcaa
    30661 ctgacgcgtc ccaaattgat tcgcgctttg gctatgctgg aaaataaagt agataccaat
    30721 ccgaaaaaga aacacggcaa tatacctttg taaaatcacg ctacggaacg ttaaaggtaa
    30781 ttaaaaaaat gcccaaagta atttgtgttg ccaaccccaa aggcggcgtt gggaaaacaa
    30841 cgacttctat caatttgtcg gcgtcattgg ccaagtttgg caaacgtgcg ttgcttattg
    30901 accttgatcc gagcggcgcg gcgagcgtcg gcgtcggttt tacgcgtgaa aacgcaacat
    30961 ttggcgtgta cgatctgtat aaacatcccg aaagtttgga tgaagcgatt gtaagaaaag
    31021 cgatatacgc cagcggtacc gaacgtctgg atgtgatgcc gtccaatgta tggacgagcg
    31081 aagaggagaa tgagcttttg aaactctctg caaaactcac gcggcttcgc attatgatta
    31141 attttgtccg taatgattat gattatttca tcatagattg ccctcctttt ctcagtaatc
    31201 tgacgttggg cgcatttacg gcatcggatt cgaccatcgt gccggttcaa tgcggttttt
    31261 ttgcgttgca tgctttggaa cgattgatga agttttatcg taaagtgcgc gaatccatta
    31321 atccatatct caagatcgaa ggtattttga tcacgatgta tgaaaaaggg acgaatgtca
    31381 gtgtaaaaac cgtacgcgat gcacgagccg tttttgaaga tttggttttt ggagtgatta
    31441 tccctaagaa cgtaaaaata ggggacgctg catttcagaa aaaaccggtt gtcacgtatg
    31501 attctacatc cgccggtgcg aaagcgtata tccaacttgc ggaggttatt ttacacaggg
    31561 atgaccccca atgggatatt caagcgcatg tacaaactcc gagcgagccc cgtaacgaat
    31621 cccagacgat cccggacgta gtatcttcca gtggggaaaa tgcttgacca aggacggcaa
    31681 aaaaggcttg taacttcctg aaaaaacaaa tacattgcac acgctgtaca cgtcgaagat
    31741 tcatacccaa attcttattc ggtttattgt gacagacaca tttttgccgc gtgttttagg
    31801 aaaaatcatt ttagcatccg cttcgccgcg acgggcacag atattaaaac aagtcggttt
    31861 tgattttgat gtgatgccga gcgccattga agaagtttct gaacatcacg aaccggtcga
    31921 gtatgcgcgc gaaattgctc aaaaaaaggc cttggatatt gctcaaaaat atcccgagcg
    31981 aattgttatc gcggcggaca cgatcgtgtg tttggacggg catattttag gaaaaccgtc
    32041 cgatgaggaa gacgcatggc gtatgctgcg ttttttgagc gggcgtaatc atcaggtata
    32101 tacggcattt gccatatctc accggtcacg caatgtgaat atggtggaac acgaattgac
    32161 acaggtgcac tttcgccggt tgcgggatga agaaatcgcc atgtacattg ccaccggagc
    32221 gccgtttgat aaagccgggg cttacggcat acaggatgcc agcgctattt ttgtagatcg
    32281 tattgatggc gatttttaca atgtggtggg gttgcctgtg acaaggttgt atcaggtatt
    32341 gacagacacg tatttggaaa aacgatgagt aatgcgtatt acaaagaact ttccggatgg
    32401 ctgcgtcagg cgcgtgcgga tcagcatata acggcggtgg cggttgcctt gcgtacccgg
    32461 atgcctttag aaacgatcga aaaaatcgaa gccggcgatt tggattttgc tacaccggtt
    32521 tttatacagg gatatcttaa aaattatgcg gacgcgatcg gccttgactc ggcggctgtc
    32581 gtgcgggaat ataaagtaat tcgtgcaaaa atagcgtatg tgacgtcgga ccgtgggcaa
    32641 gtagtagcac gtgctacggc gcaacacagc ggaaatccgg atgaactttc gtctgaaatt
    32701 ataccgtttg atcccgaatc ccatgcgttt gcgccgcact cggaattgga tcaggccgtt
    32761 cgaaaaaaaa tttcaaccga aaaatggacg aatatcatca tgatgagttc tgcggcttgc
    32821 atggtgattc tgagtctgta ttgggtcgtc tccgatgatc attcgcaaga cgaagagacg
    32881 gcggccaatt tggccaaata cgagcggaaa gcggaaacat ttaaacaagc taaaatcgaa
    32941 ccgcgtaaaa ggttgtcatt ttacttttcc gaacctattc taacggacga agaaatcaac
    33001 aggaaattgg aagtccgcga acgtgaacag caggacagta tgcgtttagc gatatatcaa
    33061 aaaaatcaaa aatggtcgca cggccagaaa gtacgcgcca tcacggtaca agaagccgcc
    33121 ttggattatg ctcaatcaat gaaataaatt ttaagacgaa gatagcgttt tcgggagatg
    33181 aaattatgct aaagaaactc aatgacatga aactacgagc caagctcatg gcgggattcg
    33241 gcgttttttc agccattttt ataactaccg taacgatcgt ttttacagtc aaacttcaca
    33301 ccgcggctgt tagccaccag tcactgatac tgcgcagctt tgccgaattg actgccaaaa
    33361 acatcagtgc gggattggat tttggcgata aaggttcggt ggtcagcgcg tttgatgcac
    33421 tcagaaccca tgccgaattc gtaatggtgc acgacgtaag cggtgaaaac tttgctactt
    33481 tttatcgcga tcctaagata gaaaatcgga ttgatcccgt ggtcaacgaa caaatcggtc
    33541 gctggcgtga agctaaaaaa agcgattgga cgtttgatgc gttggatatg aattgtatcg
    33601 tcgtaccggt atttaacagc gccggtaatg aaatcggaaa cctctcattg ggtatgtcaa
    33661 ccaagtcaat caatgatgaa ttgcaatcca atattttttt cagtttcata cttcttatag
    33721 tgactgtcgg agtgggaata ggtgtcgtgt attggattag cgggctttta gtaaggccgc
    33781 tgatgaatat tgtggagcga tttaaggaca tcgccgaagg cgaaggtgat ttaacgcgtc
    33841 ggattcacat tgatgccaaa gatgaagttg gtgagttagc tgcggcgttt aatcaatttt
    33901 tgaataagtt agaagaaatc atgcgtcagg ttgcgcggac atcggaacag atttcggtat
    33961 cggtggagtc gatcagcaag gccagttccg acgtcgccgg cggtgcagaa caacaatcca
    34021 atcaggctac attggtggcg gttgcggccg aagagatgtc cgctacgatc gtacagacaa
    34081 cagccaatac cggtgatgcg gtgcggctat cccagcaggc tgaagaagct accgatcggg
    34141 gcaaaatggt tgttgaggat acggtcaacg gattgcaaaa tatttcttcg gtggtcagtg
    34201 agtcggctca gacgttgctg gagttgggta aaactgtcgc tcagatcggt acggtgatcg
    34261 aggttatcaa cgacattgcc gaccaaatca acttattgtc gctcaatgct tcgatcgaag
    34321 ccgcgacagc cggtgatgcg ggaaaaggtt ttgccgttgt cgccaatgaa gtgaaaaact
    34381 tagcggagga tacgacacgt tccacgacgg aaatatctca gatgatcgaa aaaatccagc
    34441 atgctatggc taacgcgata cgggcgatgg agcgcggaac gatggaagta gaaaagggac
    34501 gtgctttggg gcataaaacg gccgaggcct ttgacgaaat tttgaaagcc agtgaacatg
    34561 tccgcgaaat gataacgaat atttccgttt catcccagca gcagagccat tcggccgaag
    34621 aaatttccag aaacattgaa accattgccg gtattacacg caatacagcc cagggcgtgc
    34681 gccaaataga cggtaccacg gatgctttgg tcaatcatac gtctgcacta aagacccttg
    34741 tcggacgatt taaactgaat gagcaagatc gtgcacaaaa ttacaactaa ctctccgcgg
    34801 aaaacgattt atacagacct catgcaggtt tgcgaacagc gcggttcggg ctattttgta
    34861 ctcattgatc ccgacaaaaa aacggaagcg gagacgctgc agaccgtgtc ccattgcgcc
    34921 gaagcgggtg tagacgcgat tttgatcgga agcagcctta tgctcagcag ccgatttggc
    34981 agtacgatcc aggccataaa aaaagaattt tctttacccg ttattatttt tccgggtagc
    35041 acgattcagc tttccgaata cgcggatgcg ttattatttt tgtcccttat cagcagccgc
    35101 aatccggagt ttttgataac cactcaggtg cttggggcgc cgattgttcg taagatggga
    35161 ttggaagcca tttcgacggg gtatatgctt atcgaatccg gtaaagtaac gtccgctgaa
    35221 ttcatgagta attccaaacc cataccgcgc gataaaaatg acattgctgt tgctcatgct
    35281 ttggcgggtg aatacctcgg catgaaaaca atttatctcg aagccggaag cggagcgttg
    35341 cattctgtgc cggcagaaat ggtttctgcg gtcagtgagt atgtgaatat accggtaatc
    35401 gttggcgggg gtattcgtac gcccg
//
LOCUS       DAOQGF010000023        34596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_557_length_34596_cov_6.669176, whole genome shotgun sequence.
ACCESSION   DAOQGF010000023 DAOQGF010000000
VERSION     DAOQGF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34596
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_34596_cov_6.669176"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2228)
                     /locus_tag="PK937_05265"
     CDS             complement(<1..2228)
                     /locus_tag="PK937_05265"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNH32012.1"
                     /translation="MNLSIRTRVSCGRLLKTALCGLSLTVVTGTGTAQEKEARAEYRA
                     SVKSYINSLDESFAYRYLDNEVMILDLMSTTREEFWYRRDKKITILPGTEGLPFARKE
                     MRDSIRLRMNTIKENLEGMKKSNRVLPGYTDQKALNELHMEYQRLYAMDLGMTKIDAM
                     AIRSRLVKSGTPDDIRRMVQHHLKIALRRYKEESYSAAAAELQDIYETYNTVLNEWDD
                     VLYYIGDSYYRMKDFDNAKTWFDKLVSEMTTSPYVERALYTQITMAYIKEDRSAMQKY
                     YGEYLAKAPERPKDDNRYNRAFYLAGLTFFSTSDYTTGIAALRKVSKESRYYWPARYL
                     VAHCYVGLEQYNEALEEFVHVTKIEPNNPRFTVEDQKRLKDLSLLKASFIKFEQTING
                     QKFNAVFPYMKKIQRSSEYHDAALLIASWASFKDNDIDTARIFADSLVNLYPASDYLF
                     ETKTLLGNIKVLDPTLTDRDRELYAVESYNFVAEATEAKYLADQFIAERDSTRKTLMM
                     LEDAKRVARIKNDSASFMRYDELHRQLSDALENNGYNRIREGKSAGFYQTMSVMASKI
                     RVTEAKLREARESGNKEDEEKFGKQLSDRITDFHEIYSLLDEATIREQANRCVQCVSP
                     ACVTGCPLGNNIPAWISLAAEGRFLEAAEASRATSSMPEICPRVCPQERLCEGACVIH
                     APEEPVCIGAIEKFINEYALAHGAGQIPLAPPNGKRVAVVGAGPGGLACADVLSQLGY
                     SVT"
     gene            complement(2383..2964)
                     /locus_tag="PK937_05270"
     CDS             complement(2383..2964)
                     /locus_tag="PK937_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNH32013.1"
                     /translation="MRSLCVFCSSSDAVSDVHKRSAEELGAAIVHNGWSLIYGGTTVG
                     LMGLVAISVKSHGGHVVGVIPEVIAKHGISNAQADEMIVTRDLRERKAVMESRADAFV
                     ALPGGYGTLEEIFEIVTLRQLNAIQKPAALLNINGFYDAIGEFIRSASAGQFIKPEAV
                     GLCPLIPDVPSLFDYLQHYKPITISHKWFRDVN"
     gene            complement(2977..3858)
                     /gene="speB"
                     /locus_tag="PK937_05275"
     CDS             complement(2977..3858)
                     /gene="speB"
                     /locus_tag="PK937_05275"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNH32014.1"
                     /translation="MSKTPDNFLGIEKKLSDYKTAKFAVLPIPYEATTSYGQGTRKGP
                     EAIIKASQMVEFYDEELDRDSVYDFGIATLKPVTFGKNKGEKAMKQIQQAVGKVLDDQ
                     KMPVCLGGEHTVTSPIVRAFHDRLGDDFSVLQIDAHSDLRETYHDTPWSHACVMKRIW
                     DFNKNIVQIGIRAQCIEERKLIQQNNIQTFYAKDIVGGGDEWMLEAIHALKEKVYITI
                     DCDGLDPSIIPATGTPEPGGLEWYPTMSFLRKVCERRTVIGFDIVELAPHKQFPFSDY
                     ALAKMAYKLMGYVSAKK"
     gene            complement(3927..6104)
                     /locus_tag="PK937_05280"
     CDS             complement(3927..6104)
                     /locus_tag="PK937_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNH32015.1"
                     /translation="MATHFFYSYIVLRFFIIYKTSQAMASEQTLNVAFLWHQHQPYYK
                     VAAPDGKPFFAMPWVRLHGVKDYFDIPNYLTDFPTLKQNFNLVPSLLMQIEDYTERGV
                     EDHIMRLTRLPAGQLSEHDREQIIDDFFLAHEERMIAPNPRYKELLDRRSEWRQFSVE
                     DWCDLQMWYNLCWIGESWKTKEPFRSWIQKGRGFSESDKAGVLAAHLDILRKIIPLYR
                     RLAREQQIEISVTPLYHPILPLLNDTAIAQKSNPHTTLSEKAFVYPNDAHWHIRAARE
                     FFQSRMGFAPRGMWPSEGSVSQAVIEQIAEEGFRWVATDEEILMRSESRTPGDPYQPY
                     RIRTSKSEVVVLFRDHGLSDAIGFRYAAMPPEKAAAEFVSHLEAIREDLIRRGLDPSQ
                     RLVSIILDGENCWEYYPNNGREFLRALYLRLSTSQTIETVRINDYLMKIKTPQLPEIH
                     HIHPGSWIAHNFDVWIGSHPEKNTAWEYLTETRLFLETARKSGKYTFEKLDHAWEMMA
                     IAEGSDWFWWMGDDHQARHKTRFDVLFRYFLRRVYDALGEPAHEKLFVPIMADRKKEP
                     VLHRPKALLRPRIDGKESSFYEWRDAGFYAASKDADTMSGGQVWFDSIQFGFDEHYLY
                     IRLNFSPDQITLLREKKFTVQAFFDYEGQRVVHAQEVNAHTQDWVFGEIAEWRIIRSR
                     IPLPCRFSVGLFDGRNNLETKPLRGGIPLDPDHDAMDRDVWSA"
     gene            complement(6142..6816)
                     /locus_tag="PK937_05285"
     CDS             complement(6142..6816)
                     /locus_tag="PK937_05285"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32016.1"
                     /translation="MLTPRKHLTKKELHKDPVMNKISQFTEYVQTNQQVILYGIVGLL
                     VIIATSWGYMNYSKERDAESVNKLAAPERVYFSGDYREAIRRLEKYCAEYEGTVGGGM
                     GTFYMANAYFNTDQYDFALQYFNQYLDDYADNELFVMSSLMGIAACYEGQNKYEEAAK
                     QYEKAAGKFPSSHLAPESMIGAARCYRMVNQASNAKALYEKVTKEYPETSAARDAKNA
                     LDEIGA"
     assembly_gap    7180..7279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7288..7361)
                     /locus_tag="PK937_05290"
     tRNA            complement(7288..7361)
                     /locus_tag="PK937_05290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7325..7327),aa:Arg,seq:cct)
     gene            complement(7380..7976)
                     /locus_tag="PK937_05295"
     CDS             complement(7380..7976)
                     /locus_tag="PK937_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNH32017.1"
                     /translation="MSDSPSPLSEETPVVENTTANDTVASVSEDLDAMEKPAEEQAKT
                     ETPDYETQIAALKDQLVRALADHDNFRKRKAKEFQELILTANERLLTDILPVLDDMER
                     AMKAAKDVPTDDPKVQQFVQGFEFIYKKTMKILTEKGLKPMESVGKILDPNLHDALMQ
                     LDMPDKESHTIVDEHEKGYYLNDKVIRHAKVIVSKKPE"
     gene            complement(8022..9077)
                     /gene="hrcA"
                     /locus_tag="PK937_05300"
     CDS             complement(8022..9077)
                     /gene="hrcA"
                     /locus_tag="PK937_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNH32018.1"
                     /translation="MISLSEREYIVLRKIVQSFIENGTPIGSRTLSKILPWELSAATI
                     RNIMADLEDKGFLTHPHTSAGRIPTDRAYRAYVDAMDGPISLPAEDMATISDAVLALA
                     RDISRIQDGVDEILYHSSRALAKISNELGIILAPQFHQSVFDRMRFSEVAHDRVLIEL
                     SLRSGFVKTVILDVRDSLSREHLHEISLAINERLSGLTVDEIRKSFGERLRDLRYRFS
                     DRQEALVRVLLNSAAQLFNFSTRQSVTMAGTNNILAKPDFVGRTEMQTMFALLEEKER
                     VAGILAERSTDRVIVTIGEENGVSSINGCSIITAPYRMGEGAGTVGLIGPKRMPYDKL
                     IPLVEHTARVLDKAFSI"
     gene            complement(9082..10059)
                     /locus_tag="PK937_05305"
     CDS             complement(9082..10059)
                     /locus_tag="PK937_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32019.1"
                     /translation="MKKGKISITLAGLCFLSFTGCGKSEWVTFSDTKKDQITSIVESG
                     TSIWYGTRTGLVQFDKATEKRKVYTVNNGLPDNDIKYVTRDAKGKIWIGTNLGLSSFD
                     GQNWDVYSTAESKLPGDLINGIAFENTTVWAATGGGLGRFDGTEWTPYTHTNSGLLDD
                     AAQCVVVDAAGNKWIGTQDGGVFKFKDTTWVGYDTTSYSPIKMPVVNIKSIAIEKSGI
                     LWLGTARGLMRFDGKKIELYNTKNSGIPDNLIRGLAIDGAGNKWIATTKGLVKFDGTK
                     WTTYTIANSKLPADRVTAVFVGDKDHIWVGCEGWGLTVFRETGVTLTEE"
     gene            complement(10103..11128)
                     /locus_tag="PK937_05310"
     CDS             complement(10103..11128)
                     /locus_tag="PK937_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNH32020.1"
                     /translation="MVQDIKPVYAKKDFESDQEIRWCPGCGDYSILAQVQRTLPEFQT
                     QKEKYVFVSGIGCSSRFPYYMNTYGFHSIHGRAPAIATGIKAANPELTVFVISGDGDS
                     LSIGGNHFLHVLRRNLDINYLLFNNRIYGLTKGQYSPTSETGKITKSTPMGSLEHPLN
                     PLSVALGAGATFVARAMDRDPKGLQDVIRAAIKHRGTSFVEVYQNCNIFNDGAFEHLT
                     EKDTKPDNTIWLEHGKPLVFGAKKNKGIRLDGFKPVVVNLEDGSSSVNDLLVHDMHNT
                     DPVMAFIYSQMTEAPGLPTPIGVFRSIQKSCYEEDMRTQIALAKDKMGPGDLKKLLHK
                     GDTWTVE"
     gene            complement(11196..13031)
                     /locus_tag="PK937_05315"
     CDS             complement(11196..13031)
                     /locus_tag="PK937_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNH32021.1"
                     /translation="MAAESVLNLDEVTIRLAGDSGDGMQLVGTELTNASALLGNDVGT
                     LPDYPAEIRAPAGTLPGVSGFQLHIGSFDIHTPGDEVDVLVAMNPAALKANIRSLKPN
                     GVLIVNAESFGERDLKLANCATNPLEDGTVDGYQLFRVDMSSHTKAALKDTGLDHKSM
                     ERCKNFFSLGMIAWLYNRPMDGTIKFLKEKFAKKPLLAESNILAMKAGYAYCEATEAF
                     ANSYEIPPAKLPVGKYRNISGNEAIALGFVAASQKSGLELFLGSYPITPASDILHGLA
                     KYKNFGVKTFQAEDEIAAICAAIGASFAGELGITSTSGPGVCLKSEAIGLAVMTELPL
                     VVINVQRAGPSTGLPTKTEQSDLLQAMFGRNGESPVPIIAASRPADCFECAYEAARIA
                     VKYMTPVFLLADGYIGNGAEPWLIPDSDKLNEFSAHRTFTGEKFTPYARDEKTLARPW
                     IVPGTPGFEHRIGGLEKKNIAGTVNYEPENHDYMVRIRGEKVKRIAQEWNPLSIDGPD
                     SGDLLVIGWGGTYGAIRTAVDRSREKGLSVSRIHLRWINPFPQDLDAILKRFKHVLIP
                     EINLGQLRMLIRAEYLVDAIGLNKVTGQPFSVREIENKITEIVKK"
     gene            13286..13732
                     /locus_tag="PK937_05320"
     CDS             13286..13732
                     /locus_tag="PK937_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32022.1"
                     /translation="MVLSSALLTSEDKVLRILSSYARFAEKKNVPSLFFNRLTVLDER
                     FLHLTPFCSDQQQKHFDHLMKLLIKNATNAQGAWHELYAVIEKGHMLGLHVVNFRNVG
                     SALLSAIKESSDNHCSDAIHDAWMDLITQILKRVAMMETDLGISVN"
     gene            complement(14028..19643)
                     /locus_tag="PK937_05325"
     CDS             complement(14028..19643)
                     /locus_tag="PK937_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNH32023.1"
                     /translation="MNFFTIHKHNTIRIIISSVVILFFASTLHAQSRVSFLTGEFEKS
                     GRETNEAFLRRILRASVEISFKRLSRETDRYGKTHDGFQMMYRSVPVEDAIIKVHSDH
                     RGIYAVTGETALPADIAITPKIEEAQALSKATGHIGAQAYKWQDADEEKILKEVTDNP
                     QASFYPKAETVVVNIAKEGESPDFRLAYKFDIYASKPVSRDWVYVDASSGEVVRKINR
                     IYHGTATGTADTRYAGTRTIKTDSIAPNQFRLKDLTRGNGVITYNLNHSVNDADSTHF
                     VDNDNQWTAAEYHNGNSDDGALDAHWGAEVVYDYWKNVHNQSSWDGNNAPMINFVHYN
                     NNNPNAFWDGYSVHYGQFQSVNPWTELDVVGHEFGHGIMMGIASLNYSGEGTSLHEGC
                     ADIWGALVDNYGNQKFGLNRDPWVFSEKIAFSRSFNYLMTYKSIDWDFAHNSPHGNGQ
                     VARYWFYLLAMGGSGVNKFGSSFAVNPIGIAEAEKIFYQAFKHYVTPNSIFTDFRKAT
                     ELAAQDINPAHVAVVQSAWDAVAVPGTTGSVIGCTPSGTQQNNIQVDYVTIANKGEPF
                     YNAGWSSGGYIKKDYMFNLILGKKVKMVISYPDASTKHYAMWMDTDRDGQFEEPSERL
                     AGGIISATSSTDSIQLPQTLGGTFSYGAVPIRIAVSANPINGACDNVVGEVEDYSAYL
                     NPPQVLDGYHLNIGANSGAELIGKSKLGSLETSVLFYPSDGFKNLAGSIFLNDPGYTM
                     WGFAGESYPIILRSHKFVPSAHTEYYSVWIDYDTNKVFDSNELVFQGSGQDSIKGYLK
                     IPQNMTNRVTRMRILMSRNASITDPYTAPDFGEVADFNVKLFANPLTSDGYKAPRGNC
                     WHTYSTDPTYMMSDSITIGSLILKTGRSKDGGYHDLKGTSVLFVPLNTTQAAVRLTPF
                     IDPNLQPVDSVYYNIWVDYNGDKDFNDVGEHAFFHYGKSKADGVLTIPAGLAAGLSTR
                     MRLSVSYFTDHVHADSVVNYGQVIDLGFGIQAPFTRSTPLADVNVNEDFGTTTAAILK
                     NDLIGNTTGLSFTAAPLSGGVTVNRSVDTVRIASVADFNGQVKIAMTTSDGSYTLYDT
                     ITVTVIPVNDAPYVSAGVRDTMLNEDAAKTFLMRMSDHFSDVDQDEFAVQSNVLSGSA
                     VVTISNDSLYFKPTANFNGNVIVGLMASDGSLSVSDTFAVQVLPVQDAPYRVVTLRDT
                     SFNEDVSNQLVTKLTPYFSDPDNDLLTYNVLVLSGVVSARVGQDSLYITAQADATGPS
                     SLRISATDGNTSVYDTITVTVIPVNDAPYVSADVRDTMLNEDAAKTFLMRMSDHFSDV
                     DQDEFAVQSNVLSGSAVVTISNDSLYFKPTANFNGNVIVGLMASDGSLSVSDTFAVQV
                     LPVQDAPYRVVTLRDTSFNEDVSNQLVTKLTPYFSDPDNDLLTYNVLVLSGVVSARVG
                     QDSLYITAQADATGPSSLRISATDGNTSVYDTITVTVIPVNDAPKLVVSLRDTTLTQN
                     FTRVLVEKLTDNFTDVDNGTLQYSVTSLTSGLTASLGNDSIYVTGSNLAIGTAKITVQ
                     ASDGSLTARDTLQITIIDVTAPSGFVAALATPLLPRVRFTIGGNEILSSASLTVNNTP
                     VTLTKRDSKRYSGQYDLTTLPTSLVVVGTVTDLGNNVSPAINRTIQVSTLSKPLAING
                     FELARASGVKESIVLVSEEEIGMNKSDVTIQAVTISFDETMTIRLSAPVKHRDKGRYR
                     IVRWADEEKYPDYSLISTWQECTIQYQTGVVITTIEKSGTYVLIYDSDAEVLPTEFVL
                     NQNYPNPFNPTTTIRYDIAVSGQVSLIVYNLLGQKVRTLVNGFATQGTYRLQWDGNND
                     MGQRVSSGVYLYRLESGGKRFTKRMMFLK"
     gene            complement(19646..20227)
                     /locus_tag="PK937_05330"
     CDS             complement(19646..20227)
                     /locus_tag="PK937_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32024.1"
                     /translation="MKMITWGILLCLMTACGSGGTGNEDLSKEAQQAFAEGNYEDALN
                     IFLSLAESEPVTGYTGAGWSAVRLHDYANANVYFQTIAASNDADAYAGWSLTGWALSD
                     FNSAIQRADTVLSKDADFVLKLDDGVAAADLILVQALCYYETNNFSMTLQKIKLIDSG
                     FNADLNGSDIAQVLAAKLEQLSVLFSKTLAKGQ"
     gene            complement(20653..20796)
                     /locus_tag="PK937_05335"
                     /pseudo
     CDS             complement(20653..20796)
                     /locus_tag="PK937_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(20880..22802)
                     /locus_tag="PK937_05340"
     CDS             complement(20880..22802)
                     /locus_tag="PK937_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH32025.1"
                     /translation="MSSLIIEVLHKNATERAELPKIMERDIIESSLDVIVAVDKQRRI
                     VVFNKAAEKTFGYLRSEVLGKSIRMLYADDSQSDRVVQSMKHSGKYEGQVINVRKNGM
                     LFPSFLSATQLWNDHDEVVGAMGISRELTDLKEFERKIREQSFILNLVSDAVFVLDYN
                     GRVMLWNPSAAQLYGWCETEIIDQMCLDYVFRNQTHDYHAIMHSVLKKGFWKGDIGLY
                     NKAGKKIVVESRWNLLVGLDTHGSSILVFSSDVTELRQSERTLLQNQRVETIGKLAGG
                     IAHDLNNILTTIRIGIDTLHQERASVHDASVMEVVSKSIARGSDLVKKILSYSKGQSS
                     DKKPVQLIDVVLEAVKIIQETFPKSIDFGLDMQFDLHPIMADSTQMHQLIMNLCINAR
                     DAMPQGGRLFISIRKEDCPGQRYVSGFKHFVVISISDDGVGISQENLNRIFDPFFSTK
                     EPDKGTGLGLSTVQSIAKNHGGFVKVESTVGKGTVFKVYLPYSDVIAEANIKNTKFPR
                     NTSDGEGKRILVVEDEESIRNMLKEALEEKDYLVETSQHGLDAVDKVQFNGEDYFSLI
                     IMDMNMPIMNGVLAIEEIRKRNMKVKIIASSGEMDMWMKSYLNSNLIDRTIEKPFSLE
                     KLFSVIEDVLLPVEMA"
     gene            complement(23124..23522)
                     /locus_tag="PK937_05345"
     CDS             complement(23124..23522)
                     /locus_tag="PK937_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32026.1"
                     /translation="MNPTILCVDDDAEVLKSLTILFEEDYNVLTCIHPLKALELLATN
                     NDIRLILTDHRMPDMNGLQLLKRIRETMPERAFYTIIYSGYSEVEGEMDEMIRARVIN
                     EFLTKSCPPKLLLQAVASGIKKTETLAAPV"
     gene            complement(23596..24237)
                     /locus_tag="PK937_05350"
     CDS             complement(23596..24237)
                     /locus_tag="PK937_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32027.1"
                     /translation="MISESEINIRGMIKGLAIIPLFKFIQKHDLNWHAILTQLPDRCK
                     AELSLLIKSQYTLHDFFPTPQDGLKLFSALRWYPIVISNGFLVAIARQMHFQCGVSLE
                     RGYYAIGHFTATHDLSGLLGYILRWGNILQMLEMGEKIWKHYISEGELIVISKAEGRI
                     QFDIVCSVVLSEQQYVNQGWLAALLEKKEIAQYKVETMYLQLGGGTITHKIIW"
     gene            complement(24320..26284)
                     /locus_tag="PK937_05355"
     CDS             complement(24320..26284)
                     /locus_tag="PK937_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNH32028.1"
                     /translation="MKCTFFLAALIVSTAGLSAQEKDYFDMTLEELLNVEVSVATQNK
                     LSAKESPGIISYITSDQIKVSGARDLLDVLRMVPGFDFNVTTWGAVIMSARGIADNGN
                     VSFLMDGFDLTERLYSDVYFGNHYDINQIERIEIIRGPGSVLYGGTAELAVINVITKK
                     ATDFNGGNGTVAGGLMSKTYGRANVSGSYAYSWDTDVNAFAHVFFGNANRSDRTYTDY
                     YGTSYAMKNNSGIQTYFLNGGASIGNFSFQIIVDQYNIQERDHYGITLPKKTTNHFHE
                     YLGSIKYKIHGQKWSITPSATYSHQTPWWVNNKTLDNEVWFTNWKANSDRLSMDIVAS
                     YDLNNKTNILAGANGYRDWAKYFNGSYGITGQDLSYRYWGAAAFAQIYNRNEIANVTL
                     GIRIDKHSEYGTVAVPRLALTKVMDQLHLKALYSRAFRAPGLEALRFSNGKIKPEYTT
                     AYEFETGYQWNKTLSTTVNIYDVMITDPMAYVYENNKDNYRNFGRFGTRGIEIMQQYK
                     SDLSAITFGFSHYVANKKSDVKNYQVIDYTNANPKLVNEQANLGVPQSKLSFNGLFKV
                     KKDVRISTTWVWYSSRYGYDAIDLNGNLVPKRYDPVHMLDIYVSYKSLGVGVYNIYNS
                     KYNFLMAYNGGHPPYPGMGREVYAKYEIKF"
     gene            complement(26318..26857)
                     /locus_tag="PK937_05360"
     CDS             complement(26318..26857)
                     /locus_tag="PK937_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HNH32029.1"
                     /translation="MKLLICFILILTGTPIYSQSRVEIEESLHASIMLKALTYDSKME
                     SANITVISRKNGNSNRSALVKSVDGKFIKNNAIAVNQLTYSDKVSFLKALETMKPGII
                     YLCDDLYTSQILEISEACNNLKILTISGTPDFVRSGYVSMSVIDENQKPKIILSLKKL
                     KSEGRNFQASLLKLCKIID"
     gene            complement(26861..28282)
                     /locus_tag="PK937_05365"
     CDS             complement(26861..28282)
                     /locus_tag="PK937_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH32030.1"
                     /translation="MITNEKILALFDFFRHRKGNIGLLLGLSILFFGQQTTSYFQNLS
                     AVREQAIENARQNIDYLSEEDAPIALKLENTESIEKFNKYLSKDTIYFRSLGLHDASG
                     HDLFRYIKSNHPVDSLRFALTMQDSAEGLFLVEDYIIYSKKIIDELEGKVLGKALIAY
                     SIEEKHKELNYSFLLTGTLFGCILILILIMMIKSSYKIYRSEKELAQKNLMLKDSIGQ
                     IQTAQDQLIQSEKLSAIGLMVAGIAHEINTPKASISNYGIVFSRKLSKIAKSDDLVFI
                     KETCKNIEEEILPAIELCSERIGTIVDSLLNFTRIDHKEMQYSNISKIVEGVLIILKS
                     QNRSQVLIVNNIDTSISYVCYRGQLSQVFMNIISNAIQAILTGNPERTDGVVKLNAEV
                     TNTNLIIRIQDNGPGMSESIRKKIFDPFFTTKPVGKGTGLGLSISYDIIKKHNGSIEC
                     RSEPGVGTEFIITIPDRRIHQTT"
     gene            complement(28297..28836)
                     /locus_tag="PK937_05370"
     CDS             complement(28297..28836)
                     /locus_tag="PK937_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNH32031.1"
                     /translation="MHGIVFLELRKFVEQNMGKDGWRNLLKQTEIEVDHYMPSQTYPD
                     SELYALVASACSMTGQPVNAILEAFGKFMVPNLVSVYGAFIDRKWGLIDVLENIDKTI
                     HNVVRRKNPGALPPALETHRIDIDRIEIVYRSERKMCSLGKGLILGLSELYRQNISYE
                     EATCMHTGADACRIRIIVQ"
     gene            complement(28847..29236)
                     /locus_tag="PK937_05375"
     CDS             complement(28847..29236)
                     /locus_tag="PK937_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32032.1"
                     /translation="MIDKPEILLVGVHPNFLNNLVRIFEVDFHVVSALGPMSAIEALS
                     ELQSIRVIIADAEYKTMNCLEFFKHISEQFPQRDFYKIVYAVSGDKNKIAMIEKSKND
                     FDAFVRGADSSKFIIHAVKQGLKCTTV"
     gene            complement(29821..30234)
                     /locus_tag="PK937_05380"
     CDS             complement(29821..30234)
                     /locus_tag="PK937_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32033.1"
                     /translation="MRNPIKFISFIFLIAISCSDDQSGHDNHSLDGDWTLAYTIIQYI
                     ATGDTIDVRYWDAKQIGLAITFGRDNTYTIYALLGDTIALQTNGMYSVISDNIVFDNL
                     IGTTQVMRFLKKESELCLVSYDYEVAETMFYRKKK"
     gene            complement(30725..32587)
                     /locus_tag="PK937_05385"
     CDS             complement(30725..32587)
                     /locus_tag="PK937_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH32034.1"
                     /translation="MNFTELFRSLIQKTTGVAGAEVFRRLCVEAKEALPFFEICWIGK
                     KDPLYERVTVLEFEKKDRIGSFPNSYMLSGQACEEVYKLPEPQVFDLRYSNVSVPVDI
                     PNGFYYGIPIIDMKGQVLGHFGVILSKKIDLGETEEWRIFLKKLRLEIERMEDDMRES
                     ETKNKLQAVLDSTMDGIVAFDEDYRILIFNDAAEQILKSEPGKNIGHSFKRFLTPDFR
                     SACDEYMVQCMQQKNNFQSLYFLNKHLKLRDGRGGVVEAEVNFSCIRYKNEMYFTVII
                     RDLAMQNQMQYQINQLKDEKTKLITEIKSFYNRDIAVGKSPAFEKVLDDIRKVSQTSS
                     TVLLTGETGTGKELIAATIHNTSKRQQNIFIKVNCTALPSSLIESELFGHEKGAFTGA
                     TSMRKGRFELADGGTLFLDEIGELNKDVQSKLLRVLQEREFERVGGSETIHVNVRIIA
                     ATHQNLLAKVRDGSFREDLFFRLNVFPIHVPPLRERISDLPLLAKYLIDKLSLNVGKN
                     VRFICNDALALLREYSWPGNIRELSNVLERAIILCDEETIRPSHIFLTQQQSKTENEM
                     QLVDMEIRHIKTILIKTRGKIAGRGGAAELLGINRSTLISKMKKLNIDHRQLSS"
     gene            complement(33082..33705)
                     /locus_tag="PK937_05390"
     CDS             complement(33082..33705)
                     /locus_tag="PK937_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH32035.1"
                     /translation="MISVSIVEDDSDIRNGITRYLSSQPGFKVLSFDPTAELFLARLE
                     DGPRPDIVLMDIQLPGMSGIDGIRILKRKWPEIDIAMLTVYHDEEKIFQSLCAGASGY
                     LLKNTPLVELRDALTELNAGGAPMSPQIARKVIQYFSPKPKEDDVLTAKERQVVQGLV
                     DGLSYKLIASQMNVTIDAIRFHIKNIYKKLHVNSKGELVSKFIRGQI"
     gene            complement(33758..>34596)
                     /locus_tag="PK937_05395"
     CDS             complement(33758..>34596)
                     /locus_tag="PK937_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH32036.1"
                     /translation="EWTDWSEESRAIYTNLNEGEYTFRVRAKNIYGVVGGETSYRFRV
                     LPPWYRTWWFRVMVLSVIAFILWRVYLYRVHRLLAVERLRVKIASDLHDDIGSNLSKI
                     AMYTELVQETEDPKEAKPMLKTIGDLSRNVISSMSDIVWSIDARNDRVTDLNLRMQNF
                     AHDLLDRKNITVKFESEGIDEDITLPVLVKQNVYLIFKEAVNNIYKYAEATEVVIRLV
                     LKNKRIYLEVRDNGGGLKGGVTMGGHGMKNMKMRAETLGGLFQSSSDQGVTIRVEIPI
                     RD"
BASE COUNT         9412 a         8108 c         6974 g        10002 t
ORIGIN      
        1 gtcaccgagt agccgagttg tgatagcacg tccgcacacg ccaacccgcc cggccccgcg
       61 ccgacgacgg cgacgcgctt gccgttgggc ggcgcgagag gaatctgtcc cgcgccgtgg
      121 gcgagcgcgt attcgttgat gaacttctca atcgcgccga tgcacacggg ctcttccggc
      181 gcgtgaatga cgcacgcgcc ttcgcagagc cgttcctgcg ggcaaacgcg cgggcagatt
      241 tccggcatcg aactcgtcgc gcgtgacgct tccgcggctt cgaggaagcg cccttccgcg
      301 gcgagggaaa tccaggcggg gatgttgttg ccgagcgggc aaccggtaac gcaagccggc
      361 gaaacgcatt gcacgcagcg gttggcctgc tcgcggatgg tggcctcgtc gagcagcgag
      421 taaatctcgt gaaaatccgt gatccgatcc gaaagttgtt tgccgaattt ttcttcgtct
      481 tccttgttgc cgctttcacg cgcttcgcgc aacttggctt ctgttacgcg aattttgctg
      541 gccatgaccg acatagtttg ataaaaaccg gccgacttgc cttcgcgaat acggttataa
      601 ccgttgtttt ccaatgcgtc actgagttgg cgatgcaact cgtcataccg cataaagctc
      661 gcgctatcat ttttgatgcg cgcgacacgt ttcgcatctt cgagcatcat cagtgttttg
      721 cgtgtcgaat cgcgttcagc gataaactga tccgcgagat acttggcttc cgtagcctcc
      781 gcgacaaaat tatatgattc gaccgcatat aactcgcgat cgcgatccgt cagcgtagga
      841 tccaacactt tgatattacc aagcagtgtt ttggtttcaa ataaatagtc gctggcagga
      901 tataagttaa ccaacgaatc cgcgaatatg cgcgccgtat caatatcatt gtctttaaac
      961 gaagcccaag acgcgatcag cagtgcggca tcatgatatt ccgaactgcg ctgtattttt
     1021 ttcatgtacg gaaatacggc gttgaatttt tgtccgttga tcgtttgctc gaacttgatg
     1081 aaagatgctt tgagcaacga gagatctttc aggcgttttt gatcttctac ggtaaatcgc
     1141 ggattgttcg gttcgatttt cgtaacatga acaaattctt ccagcgcttc attatattgt
     1201 tccaacccaa catagcaatg cgctaccaaa tatcgcgcag gccaatagta acgcgattct
     1261 ttggatactt tgcgcagtgc ggctatcccg gttgtataat ctgaagtgga aaaaaatgtc
     1321 aaaccggcca aataaaaagc tcggttgtaa cggttatcat ctttaggacg ttccggagct
     1381 ttagcgaggt attcgccgta gtacttttgc atcgcggaac gatcttcttt gatgtacgcc
     1441 atcgtaatct gtgtgtacaa tgcccgttct acgtatggcg aggtggtcat ctcggaaacc
     1501 aatttatcga accaggtttt cgcgttatca aaatctttca tccggtaata actgtcaccg
     1561 atataataaa gcacatcatc ccattcgttg agaacagtat tgtacgtctc ataaatatcc
     1621 tgcaactccg cagctgcggc cgagtaactt tcttccttat agcggcgcag tgcgattttg
     1681 aggtggtgtt ggaccatgcg acgtatatca tcgggtgttc cgctcttgac caatcgggag
     1741 cgaatagcca tcgcgtctat tttggtcata ccaagatcca tcgcatacaa acgctgatac
     1801 tccatgtgga gctcattcaa tgctttctga tcggtatatc cgggaagaac gcgattactc
     1861 tttttcatac cctcaagatt ttctttgatg gtattcatgc gcaaacgaat cgagtcacgc
     1921 atttctttgc gtgcaaaagg taaaccttcc gttccgggga gaattgtaat ttttttatca
     1981 cgacggtacc aaaactcttc acgcgtcgta gacatcaggt caagaatcat cacctcgtta
     2041 tcaaggtaac gatacgcaaa actttcgtcg aggctgttta tgtagctttt gacgctcgca
     2101 cggtattcag cgcgggcttc tttttcttgc gccgtgccag taccggtcac cactgtcaaa
     2161 ctcagtccgc agagcgcggt cttcaataaa cggccacacg atacgcgggt gcgaatactc
     2221 aaattcattc cgaccttcct gtgaagatta gcaaaagacc gtcgtcggcc tttggaattt
     2281 aacctaagga tttgttttat ctaatttcag cgaatttagg cgttttaaaa agtgaagtca
     2341 agttatttta ttaagttacg ttaaacggac ggcggccgta tttcagttca cgtcgcggaa
     2401 ccatttgtgt gaaatagtga tcggtttgta atgttgcaga taatcaaaaa gtgaaggcac
     2461 atccgggatt aatgggcaca aacctaccgc ttcgggcttg ataaactgac cggcactggc
     2521 cgaacgaata aattcaccga tcgcgtcata gaacccattg atattgagca gcgccgcagg
     2581 cttttgtatg gcattgagtt ggcgtaatgt aacaatttcg aatatctcct ccaacgtacc
     2641 gtacccgccg ggcaaagcca caaacgcatc ggcacgcgat tccatcacgg ctttacgttc
     2701 acgcaaatcg cgcgtcacga tcatctcatc cgcttgtgca ttactgatac catgcttggc
     2761 tatcacttcg ggaataacac cgactacatg tcctccatgg cttttgacac tgatcgctac
     2821 aaggcccatt aagccgacgg tcgttccgcc atagatcagt gaccatccgt tatggacgat
     2881 cgccgctcct aattcttccg cggatcgctt atgtacatcg ctaacggcat cactggacga
     2941 acaaaacaca cataatgaac gcatcacgcg taaacctcat tttttagcag acacatagcc
     3001 cattaattta taagccattt tagctagtgc ataatcggaa aacgggaact gtttgtgggg
     3061 cgccagttcc acgatatcaa aaccgatcac cgtgcggcgt tcgcatactt tgcgaagaaa
     3121 cgacatggtg ggataccatt ccaaaccgcc cggctcgggc gtcccggtcg ccggtataat
     3181 actgggatcc agtccatcgc aatctatcgt gatatacact ttttctttca atgcgtgaat
     3241 cgcctcgagc atccattcgt ccccgccgcc gacaatatcc ttcgcgtaaa aagtttgaat
     3301 gttgttctgc tgaatcagtt tccgttcctc gatacattgc gcgcgaatac cgatttgaac
     3361 aatgttttta ttaaaatccc aaatacgctt cataacacat gcgtgactcc acggcgtatc
     3421 atggtacgtt tcgcgcaaat ccgaatgcgc gtcaatctgt agtacggaga aatcatcgcc
     3481 taagcgatcg tggaatgcgc gtacgatcgg cgaggttacc gtatgttcgc ccccgaggca
     3541 tacgggcatt ttttggtcat ctaaaacttt tccaacggcc tgctggattt gtttcatcgc
     3601 tttttcacct ttgttctttc caaaggttac cggtttaagc gtggcaatgc caaagtcata
     3661 tacgctatcg cgatccagct cttcatcata aaactcaacc atctgcgaag ctttaatgat
     3721 cgcttcgggg cccttacgcg ttccctgacc ataactggtt gttgcttcgt aaggaatcgg
     3781 caaaacggca aatttggccg ttttgtaatc cgataatttt ttttctatgc ccaaaaagtt
     3841 atcgggtgtt ttgctcatga tatttctccg aaattaaacg aggtgatttt tataaatcta
     3901 tgccggttac catacatcaa aatatatcat gcagaccata catcacgatc catcgcgtca
     3961 tgatccggat cgagtggaat accgccgcgc aagggcttgg tttccagatt attacgccca
     4021 tcaaacagac ccacggaaaa acgacacggt aaaggaatgc gcgaacgtat tatgcgccac
     4081 tctgcaattt caccgaagac ccaatcctga gtatgtgcat tcacctcctg tgcatgcact
     4141 acgcgctggc cttcataatc aaaaaaagcc tgtacagtaa attttttttc gcgcaacaaa
     4201 gtaatttgat cgggtgaaaa attcaaccgg atgtagagat aatgttcatc aaatccgaat
     4261 tgaatgctat cgaaccaaac ttggccgccg ctcatggtat cggcatcttt tgatgcggca
     4321 taaaaaccgg catcgcgcca ttcgtaaaac gaagattctt tgccgtcaat acgcggtcgg
     4381 aggagcgctt tggggcgatg cagcacaggt tcttttttac gatcggccat gatcggaaca
     4441 aataattttt catgcgcggg ttcgcccaat gcgtcgtata cgcgacgtaa aaaataacga
     4501 aacagcacat caaatcgcgt tttgtggcgc gcctgatgat cgtcgcccat ccaccaaaac
     4561 caatcgctgc cctccgcgat agccatcatc tcccatgcat gatccagttt ttcaaacgta
     4621 tattttccgc tcttgcgcgc cgtttcgaga aataaacgcg tttccgtgag atattcccac
     4681 gccgtatttt tttccggatg cgaaccgatc cagacgtcga aattatgagc aatccatgaa
     4741 cccggatgaa tgtgatgtat ttccggcaat tgcggcgttt ttattttcat caaataatca
     4801 ttgatgcgaa cggtctctat ggtttgcgac gtggataggc ggagatataa agcgcgtagg
     4861 aattcgcgac cgttgttggg ataatattcc cagcaatttt cgccatccag aataatgctc
     4921 accaatcttt gcgacggatc cagcccgcga cgtatcagat cttcgcgaat ggcttccaaa
     4981 tgcgacacaa actcggcggc ggccttttcc ggcggcatgg cggcataacg aaatccgatc
     5041 gcatcggaga gaccgtgatc gcggaatagc acgactactt cggacttgga agtacggatg
     5101 cgatacggtt gatacggatc gcccggagtt cgcgactcgg agcgcatcaa aatttcttca
     5161 tctgtcgcaa cccatcggaa accttcttca gcgatttgct caatcacagc ctgactgacg
     5221 ctgccttcgg acggccacat cccgcgcggt gcaaaaccca tgcgcgactg gaaaaattcg
     5281 cgtgcggcac ggatatgcca atgtgcgtca ttcggataaa caaatgcttt ttccgaaagc
     5341 gtcgtatgcg ggttggattt ttgcgcgatg gccgtatcgt tcaataacgg caaaatcgga
     5401 tggtacagcg gtgtgacgga aatttcgatt tgttgctcgc gcgccaaccg ccggtagaga
     5461 ggaatgattt ttcttagaat atccaaatgc gccgctaaaa cgccggcttt gtcggattcg
     5521 ctaaaaccgc gccccttttg gatccacgaa cgaaacggtt ctttcgtttt ccacgattcg
     5581 ccgatccagc acagattgta ccacatctga agatcgcacc agtcttcgac agaaaactgc
     5641 cgccactcgg agcgtcggtc taataattct ttataccgcg gattgggtgc gatcattcgc
     5701 tcttcgtggg cgagaaaaaa gtcgtctatg atttgctcgc gatcatgttc gcttaattga
     5761 ccggccggca agcgcgtcaa tcgcataata tgatcttcga caccgcgttc ggtataatct
     5821 tcaatctgca tcagcagcga aggcaccaaa ttaaaatttt gtttgagcgt agggaagtcg
     5881 gtgaggtaat taggaatatc gaaataatct tttacaccat gcaggcgcac ccaaggcatt
     5941 gcaaaaaatg gtttgccatc cggcgcggcc actttgtaat acggctggtg ctggtgccac
     6001 aagaaagcga cgttaagagt ttgttccgat gccatcgctt gtgaagtttt ataaataatg
     6061 aaaaaccgta aaacaatata cgaataaaaa aaatgggttg ccacaaaagc aacccattaa
     6121 tataactttt aaaatttacg cttaggcgcc gatctcatcc aacgcattct tagcatcacg
     6181 cgcggcgctg gtttcaggat attctttggt cactttttca tacaacgctt tagcatttga
     6241 cgcttgattg accatgcggt agcaacgcgc tgctccgatc atgctttcgg gtgccaaatg
     6301 ggaactggga aatttaccag ctgctttttc gtattgtttg gccgcttctt cgtatttatt
     6361 ctgcccttca taacaagccg caatacccat aagcgacgac atcacaaaaa gttcattatc
     6421 cgcatagtcg tcgagatact ggttgaaata ctgtaacgca aaatcgtatt gatcggtatt
     6481 aaaataagca ttggccatat aaaaagtacc catgcctcct ccgacggtac cttcgtattc
     6541 agcacaatac ttttcgagac gacgaatcgc ttcacggtaa tcaccgctga aataaacacg
     6601 ctccggtgcg gctaatttat tgacggactc tgcatcacgt tctttactat aattcatata
     6661 gccccaagac gtggctataa tgaccagtaa accgacaata ccgtacagga taacctgttg
     6721 attggtctgc acgtattcgg tgaactgtga aattttattc atcaccggat ctttatgcag
     6781 ttcttttttt gtaagatgtt ttcttggcgt taacatgcgt aattcacttc attctgtggt
     6841 ctaattgatt tttaaagcgc cccataatac aaaacgggct tcttgcacgc aagtaaaatt
     6901 aacagaaact aatttttatt ctagttggtt aaaaatgatt ctacggctca ataagtcctc
     6961 tatcaatatt gtgttgttac aattataaca tcaagcctgc ggaaacgacg accattgatc
     7021 ttgcgctgat tgtaccctca atcacgccgt tggtgtgacg accattgatc ttgcgatgat
     7081 tgtaccctca atcacgccgt tggcgtgatg accattgatc ttgcgctgat tgtaccctcg
     7141 acacgatttg aacgtgcgac caacggttta ggaaaccgcn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnng ctgattgtac cctcgacacg atttgaacgt gcgaccaacg
     7321 gtttaggaaa ccgctgctct atccagctga gctacgaggg cgaatatcga ataaaaaact
     7381 tattccggtt ttttactgac gatcactttg gcatgacgaa tgactttatc gttgagataa
     7441 taaccttttt catgctcgtc cacgatggta tgggattctt tgtccggcat atccagctgc
     7501 atcaatgcat cgtgcagatt gggatctaat atttttccga ccgactccat aggctttaaa
     7561 cctttttccg tcagtatctt catcgtcttt ttatagataa attcaaagcc ttgtacaaat
     7621 tgctgcacct taggatcatc cgtaggtaca tcttttgctg ctttcattgc gcgttccata
     7681 tcatccagca caggaagaat atcagtcaac aaccgttcat tggcggtcag gatgagttcc
     7741 tgaaattctt tggctttacg ttttctgaaa ttatcatgat ccgccagcgc acgcaccagt
     7801 tgatctttca gcgcagcgat ctgggtttca taatccgggg tttctgtttt agcttgttcc
     7861 tcggcaggtt tttccattgc atccagatct tccgacacgg atgcaacggt atcgttagcg
     7921 gtcgtgtttt ctacgacggg tgtttcttcg gataaggggg acgggctgtc agacatagtt
     7981 ttaaaaacgt gttttaattg gttggtgaaa tagttatcag tttaaattga aaaagcttta
     8041 tccaatacac gggccgtatg ttcgacgagc ggtattagtt tatcatacgg catacgttta
     8101 ggaccgatga gtcccacggt tccggcgcct tcacccatac ggtaaggcgc cgtgatgatg
     8161 ctgcagccat tgatcgaaga tacgccattc tcttctccga tcgtcacgat tacgcggtcc
     8221 gtcgagcgct cggccaagat ccccgctacg cgctcttttt cctccagtaa ggcaaacatc
     8281 gtctgcatct ccgtacgtcc tacaaaatcc ggtttggcta aaatattatt cgtccctgcc
     8341 atcgtcacgg actgacgcgt gctaaaatta aatagctgcg ccgccgaatt gagcaacaca
     8401 cgtaccaacg cttcttggcg gtcactgaaa cgataacgta aatcccggag tcgttcaccg
     8461 aatgatttgc gaatttcatc tacggtgaga ccgctcaggc gttcgttgat ggccaaactg
     8521 atctcgtgca agtgttcgcg cgacaaactg tcacgcacat caagtataac cgttttgaca
     8581 aatccgctac gcagggaaag ttcgatcaat acccggtcgt gtgcaacttc cgaaaaccgc
     8641 atccgatcaa atacgctctg atgaaattgg ggtgcgagga taatgccgag ttcgttagaa
     8701 attttggcca acgctctgga ggagtgatat aaaatctcgt caacaccgtc ttgtatgcgt
     8761 gaaatgtcac gtgcgagcgc caatacggca tccgatattg tagccatgtc ttcagccggt
     8821 aacgatatcg gtccatccat cgcatccaca tacgcacggt atgctcgatc tgtcggaata
     8881 cgaccggccg atgtgtgcgg atgcgtaaga aaacccttgt cttccagatc cgccataatg
     8941 ttacgtatcg tcgccgcact caattcccac ggcaaaattt tggacaacgt gcgtgaacct
     9001 atcggcgttc cgttttcgat aaacgattga acgattttgc gtaacacaat gtattcgcgt
     9061 tctgagagag atatcatcgc attactcttc tgtcaatgtc acgccggttt ctctgaatac
     9121 cgtgagtccc cagccttcgc agcccaccca aatatgatct ttatcaccga cgaaaacagc
     9181 cgtcacacga tccgccggta gtttcgaatt ggcaatggta tatgtagtcc actttgtccc
     9241 atcaaacttg acaagacctt tggtcgtagc gatccatttg tttccggcac cgtcaatcgc
     9301 caaaccccga ataagattat ccggaatgcc ggaattcttc gtattataca attctatttt
     9361 ttttccatca aatcgcatca gaccgcgcgc cgtaccaagc cataggatgc ctgacttttc
     9421 gatcgcaata cttttgatgt tgaccaccgg cattttgatg gggctatagg acgtcgtatc
     9481 gtaaccgacc catgtcgtgt ctttaaattt gaatacaccg ccgtcttgtg tgccgatcca
     9541 tttatttccg gctgcatcca cgacaacaca ttgagccgcg tcatccaaaa gaccggaatt
     9601 ggtatgagtg tacggcgtcc attcggtacc gtcgaagcgc ccaagtcctc cgcccgtcgc
     9661 cgcccacact gtcgtgtttt caaacgcgat accattgatc aaatcgccgg gcaatttcga
     9721 ttcggcggtg gaataaacgt cccagttttg accgtcaaaa gaagacaatc caaggttcgt
     9781 gccgatccat attttacctt tggcgtcgcg ggtcacatat ttgatatcgt tatccggcag
     9841 gccgttattg acggtataga ctttacgctt ttcggtggct ttgtcaaact gtacaagacc
     9901 ggtgcgcgta ccataccaaa tggacgtacc gctctcgacg atggaagtga tttgatcttt
     9961 tttagtatcg gaaaatgtca cccattccga tttgccgcaa ccggtaaacg acaaaaaaca
    10021 aagaccggct aacgttatgc taatttttcc ttttttcatg accaactctc cttttcaaaa
    10081 tccctaaagc ccacgggggt cgttattcga ccgtccacgt atcaccttta tgcaaaagtt
    10141 ttttcagatc accgggtccc attttgtctt tggccaaggc gatctgagta cgcatatctt
    10201 cctcataaca acttttttgt atgctccgga aaacgccgat cggtgtggga agtcccggcg
    10261 cttcggtcat ctggctgtat ataaatgcca tcaccggatc cgtattgtgc atgtcgtgca
    10321 caagcagatc attgacagag ctcgaaccgt cttccagatt gacaacaaca ggtttgaatc
    10381 cgtcaaggcg gatcccttta ttttttttgg cgccaaacac aagcggtttg ccatgctcaa
    10441 gccatatcgt attatcgggt ttggtatctt tttccgttaa atgctcaaac gcgccgtcgt
    10501 tgaatatatt gcaattttgg tacacttcga cgaacgacgt accgcggtgt ttgatcgcag
    10561 cgcgaatcac atcctgaagt cctttgggat cacgatccat cgcgcgcgct acgaatgtcg
    10621 ctccggcacc gagtgctacg gataacggat taaggggatg ttccagtgag cccatcggcg
    10681 tggatttggt aattttgccc gtttcggaag tcggcgaata ttgacctttg gtcaatccat
    10741 aaatccggtt attaaacagc agataattga tatccaaatt acgacgcaaa acgtgcagga
    10801 agtggttacc gccgatggaa agcgaatctc cgtcgccact gatgacgaac acggtcagtt
    10861 ccggattggc ggctttgatg ccggtcgcaa tcgcgggcgc acgtccgtga atcgagtgaa
    10921 acccgtaggt attcatataa tacggaaacc ggctggagca accgataccc gacacaaata
    10981 catatttttc tttttgtgtc tgaaattccg gcaaggtacg ctgtacttgt gccaagatgg
    11041 agtagtcgcc gcatcccggg caccagcgaa tttcctgatc ggattcaaaa tctttttttg
    11101 cgtaaacggg tttgatatcc tgtaccatcg tataaacctg cttctttttc ttaactctgc
    11161 accgcgacgt tattaacaac gatcgggcag aaaaattatt tctttacaat ttccgtaatt
    11221 ttattttcga tttcacgtac gctaaacggc tgaccggtca ccttattgag cccgatcgca
    11281 tccacgaggt attccgcacg gatcagcatg cggagttgac cgagattgat ttcaggaatc
    11341 aacacgtgtt tgaatcgttt gagaatcgcg tcaagatctt gcggaaacgg attgatccaa
    11401 cgcagatgaa tacgggacac gctcaatcct ttttcacggc ttcgatcaac ggctgtacga
    11461 attgcgccgt aggtgccgcc ccaaccgatc accaaaagat cacccgagtc cggtccgtct
    11521 attgataacg gattccactc ctgcgcgata cgtttcactt tttcgccgcg aatgcgcacc
    11581 atataatcat gattttccgg ttcgtaattg accgtgccgg cgatattttt tttctccaag
    11641 ccgccgatgc gatgctcgaa tcccggcgta cccggtacaa tccacggacg tgcaagcgtt
    11701 ttttcatcac gagcatacgg cgtaaacttt tcaccggtaa atgtccgatg cgctgaaaat
    11761 tcgttgagtt tatcggaatc gggaatgagc cacggttcgg cgccgttacc gatatatccg
    11821 tcagcgagca gaaataccgg cgtcatatat ttgaccgcga tacgtgctgc ttcgtaggcg
    11881 cattcaaaac aatcggccgg acgcgaggcg gcaataatgg gcacgggcga ttcgccatta
    11941 cgtccaaaca tcgcttgcaa aagatcggac tgttcggttt tggtaggaag ccctgtgctc
    12001 ggaccggcgc gttgcacatt gatcaccacc agcggaagct cggtcatcac ggcaagtcct
    12061 atagcttcgc ttttgagaca gacaccgggg ccggacgtac tcgtgatgcc gagttcaccg
    12121 gcaaatgaag cgccgatcgc tgcgcatatc gccgcgattt catcttcggc ctgaaacgtt
    12181 ttaacgccaa aatttttata cttcgcaagg ccgtgcaaaa tatcgctggc cggcgtgatc
    12241 gggtacgaac ctaaaaacaa ttccaatccc gatttttgag aagccgctac aaaacctaaa
    12301 gcgatcgctt cgtttccgct gatattgcga tattttccga cgggtaactt ggccggaggg
    12361 atttcatacg aattagcaaa agcttctgtc gcttcgcaat acgcataacc cgctttcata
    12421 gcgaggatat tggactcggc gagtaaaggt tttttggcaa atttttcttt aagaaatttg
    12481 atcgtaccgt ccatcggacg attgtacaac caagcgatca tgccgagtga aaaaaagttt
    12541 ttgcaacgct ccatggattt gtgatcaagc cccgtatctt taagcgccgc tttggtatga
    12601 gacgacatat ccactctaaa aagctgataa ccgtccaccg taccgtcttc gagcggattg
    12661 gtcgcacagt tggccagttt cagatcgcgt tcgccaaacg attccgcatt gacgatcaat
    12721 acgccgttgg gtttgagaga tctgatattg gctttgagcg cggcaggatt catcgcgact
    12781 aggacgtcca cttcatcgcc gggcgtatgg atatcaaaac ttccgatgtg caattggaaa
    12841 ccgctcacac cgggcaatgt accggcaggt gcgcgaattt ccgcggggta atccggcagt
    12901 gtaccgacat cgttaccgag caacgccgat gcattcgtca attccgtacc gacaagctgc
    12961 ataccatcac cggagtcacc ggctaaacgg atcgttactt cgtccagatt caagaccgat
    13021 tcagcggcca tatagcatta acacctttcc aagaatgagt aacttgactt ttgctgtagt
    13081 attataccac aacgtgacaa cataatacgc aggcatcaaa agtgcaagag ccagccggga
    13141 atagtttgat gatttttata ttcatgttcc gcatccggga tttgcagtcc tgcatattca
    13201 tgtagtttgc cgatcgcatc acttttttta tacttttccc aaagaaacgt atttttatcc
    13261 cattaattat ctcatcgaaa aaaatatggt actatcctcc gctctcctga catccgaaga
    13321 taaagtatta agaattttgt ccagctacgc gcgatttgca gagaaaaaaa acgtaccgtc
    13381 tctatttttt aatcgattga ccgtattgga tgagcgcttt ttacacctca cgccgttctg
    13441 ctcagatcaa caacaaaaac attttgatca tcttatgaag ctcctgatca aaaatgcgac
    13501 caacgcccaa ggcgcttggc atgaattgta tgctgtgata gaaaagggtc acatgctggg
    13561 attacatgta gtcaattttc gtaacgtggg gagcgcttta ttatcggcta taaaagagtc
    13621 gtcggataat cattgttccg acgctataca tgatgcttgg atggatttga tcacgcaaat
    13681 attaaaacgt gtcgcgatga tggaaacgga tttgggtatt tcggtaaatt agtcacacat
    13741 aactcaactt atccggtaaa actaacaatc agcgtcagta tcaatcctat catcacgaga
    13801 cccgaggcaa taaccagtat cgtctgtatc atcggtgatt gtagatccat gttgcctgct
    13861 tattttgcta ttttcataaa atttgctctg ataatttaga gttaattttt tcgaaacact
    13921 actgcatgac aatgtggttt gatcattgct attcatgcat aaaaaaacgg cgttactgag
    13981 gttactaccc gttcacgccg ccggttctca tcaagtgagg attgttttta cttcaagaac
    14041 atcattcgct tagtgaatcg ttttcctccg gattccagac ggtaaaggta cacaccggat
    14101 gaaacgcgtt ggcccatatc attattacca tcccattgca aacgatacgt cccttgcgtc
    14161 gcgaaaccat tgaccagcgt acgcaccttt tgcccgagca gattatagac gataagcgac
    14221 acttgccccg acaccgctat atcgtaacga atcgtcgtcg tcgggttaaa cgggttggga
    14281 taattttgat tgagtacaaa ctccgtcggc aatacctcgg catcggaatc gtagattaac
    14341 acatacgtgc cggacttttc tatcgtcgtt atgaccacgc cagtttggta ttgtatcgtg
    14401 cattcctgcc aagtagaaat cagcgaatag tcgggatatt tttcttcatc cgcccatcgc
    14461 acgatgcgat accgaccttt gtcccgatgc tttacaggcg cggacagacg aatcgtcatc
    14521 gtttcatcaa aactgatcgt cacggcttgt attgttacat cagatttatt cataccgatt
    14581 tcttcttcgc ttaccagtac gatactctct tttacccctg aagcccgtgc gagttcgaaa
    14641 ccattgatcg ccagcggttt gctgagcgta ctcacctgaa tcgtacgatt gatcgccggg
    14701 ctcacgttat tgcccaaatc cgtgaccgtg cctacaacaa cgagtgacgt cggcagtgtc
    14761 gtgagatcgt attgaccgga ataacgtttg ctgtcccgct tggtaagcgt gaccggcgta
    14821 ttattgaccg ttaatgaggc gctactcaat atttcattac cgccgatcgt aaaacgaacg
    14881 cgcggcaaaa gcggcgtggc aagagccgca acaaaacccg atggcgcggt cacatctatt
    14941 atggttatct gtaatgtgtc cctggccgtt aacgatccgt cactggcttg tacggtgatt
    15001 ttagccgtac cgatagccag attagaaccg gtaacataga ttgagtcatt gcccaatgaa
    15061 gcggtcagcc cgctcgtcag cgatgtgacc gaatactgta gcgtaccatt atccacgtcg
    15121 gtaaaattat cggtcagctt ttcgaccagc acccgcgtaa aattctgagt cagtgtcgta
    15181 tcgcgcaatg atacgactag cttcggcgca tcattgacgg gaatcaccgt aacagtgatc
    15241 gtatcataca cggatgtatt tccatctgtg gccgatatgc gcagtgagct tggacccgta
    15301 gcatcggctt gtgcagtaat gtacagagag tcctgaccta cgcgtgccga tacgacaccg
    15361 gatagtacta atacattgta cgtcagaaga tcattgtccg gatctgaaaa atacggcgtg
    15421 agtttagtaa ccaactgatt actcacatcc tcattaaatg atgtatcgcg caatgttaca
    15481 acacgatacg gtgcgtcttg gacaggaagc acctgcacgg caaaggtatc tgatacggac
    15541 agactaccat ccgatgccat gagcccgacg atcacatttc cattgaaatt tgccgtcggc
    15601 ttgaaataca aggaatcatt actaatggtt actactgcac taccggacaa cacgttggat
    15661 tggactgcaa attcatcctg atccacatcc gaaaaatgat cactcatacg catcaaaaac
    15721 gttttcgctg cgtcctcgtt aagcatcgta tcacgaacat ccgcactaac atatggtgca
    15781 tcattgacgg gaatcaccgt aacggtgatc gtatcataca cggatgtatt tccatctgtg
    15841 gccgatatgc gcagtgagct tggacccgta gcatcggctt gtgcagtaat gtacagagag
    15901 tcctgaccta cgcgtgccga tacgacaccg gatagtacta atacattgta cgtcagaaga
    15961 tcattgtccg gatctgaaaa atacggcgtg agtttagtaa ccaactgatt actcacatcc
    16021 tcattaaatg atgtatcgcg caatgttaca acacgatacg gtgcgtcttg gacaggaagc
    16081 acctgcacgg caaaggtatc tgatacggac agactaccat ccgatgccat gagcccgacg
    16141 atcacatttc cattgaaatt tgccgtcggc ttgaaataca aggaatcatt actaatggtt
    16201 actactgcac taccggacaa cacgttggat tggactgcaa attcatcctg atccacatcc
    16261 gaaaaatgat cactcatacg catcaaaaac gttttcgctg cgtcctcgtt aagcatcgta
    16321 tcacgaacac ccgcgctaac atacggcgca tcattgacgg gaatcaccgt aacggtgatc
    16381 gtatcgtata gtgtgtagct accatccgaa gtggtcatcg cgattttgac ttgaccgtta
    16441 aaatcggcta cgctcgcgat acgaacggtg tccacgctcc gattgacggt cacacctccg
    16501 gataaaggtg ccgcagtaaa agaaagtccg gtggtgttac caataagatc gttctttagt
    16561 attgccgcgg ttgtcgtgcc gaaatcttcg ttcacattca catcggctaa cggcgttgaa
    16621 cgtgtgaacg gtgcctgtat gccaaatccc aaatctatca cttgtccgta attaaccacc
    16681 gaatcggcat ggacgtggtc ggtaaagtag ctgacgctca aacgcatgcg tgtacttaag
    16741 ccagcggcaa ggccggccgg tatcgttaaa accccgtcgg cttttgattt cccgtaatga
    16801 aaaaatgcat gctcacccac atcattaaaa tctttgtcac cattgtaatc tacccagata
    16861 ttgtagtata ccgaatctac cggttgcaga ttgggatcaa tgaatggagt caatcgcacc
    16921 gcggcctgag ttgtatttaa cggcacgaac aatacggatg ttcctttcaa atcatgatat
    16981 ccgccatctt tgctgcgacc ggttttcaga atcaatgaac cgatggtaat ggaatcggac
    17041 atcatgtacg taggatccgt actataagta tgccaacaat tacctcgtgg cgctttgtag
    17101 ccgtcggaag tcagaggatt ggcaaaaagt ttaacattaa aatccgctac ttcgccaaaa
    17161 tccggagccg tataaggatc ggttatagat gcattgcgac tcatgagtat gcgcatacgg
    17221 gtaacccgat ttgtcatatt ttgaggaatt tttaaatatc ctttaatcga atcctgacca
    17281 cttccttgga atactaattc gttcgaatca aataccttat tggtatcgta gtcaatccat
    17341 actgaataat attctgtgtg ggctgagggc acaaatttgt ggctcctcaa gataatcgga
    17401 tacgattcac cggcaaaacc ccacatggta taaccggggt cgttcaagaa tatagaaccc
    17461 gctaaatttt tgaatccgtc acttggataa aacaaaaccg acgtttccaa agaacccaac
    17521 ttcgatttcc caataagttc tgcaccggaa ttggcgccga tatttaagtg gtaaccgtct
    17581 aaaacttgtg gcggattcaa gtatgcggaa tagtcttcga cttcaccgac gacattgtca
    17641 catgcgccgt tgattggatt cgcactcacg gctatacgta tcggcacagc accatacgaa
    17701 aacgtacctc cgagcgtttg tggcaattga atggaatccg tcgatgaggt tgctgaaatt
    17761 attccgccgg ctaatcgttc agaaggttcc tcaaactggc catctctatc ggtatccatc
    17821 cacattgcat aatgcttcgt cgatgcatcg ggatatgaaa taaccatttt tacttttttg
    17881 ccaaggatca ggttaaacat ataatctttt ttgatgtatc cgccagacga ccaacccgca
    17941 ttataaaagg gttcgccttt attggcgatg gtaacataat ccacttgaat gttgttttgt
    18001 tgagttcccg acggtgtaca acctatgaca ctgccagttg ttccgggaac agcgacagcg
    18061 tcccatgcgg attggacaac ggcaacgtgt gccggattga tgtcctgtgc tgccaattct
    18121 gtagcttttc taaaatcggt aaaaatacta tttggtgtta catagtgctt aaaagcctga
    18181 taaaagatct tttctgcttc ggcaataccg attgggttta ctgcgaatga agaaccaaac
    18241 ttattcactc cagatccgcc catggcaagg agataaaacc aataccgcgc aacttgcccg
    18301 ttaccatgcg ggctgttgtg tgcaaaatcc caatcgatcg atttataagt catcaaataa
    18361 ttaaacgaac gactgaatgc gattttttct gagaaaaccc aaggatctcg attcaatccg
    18421 aatttttgat ttccataatt gtcaacaagc gctccccaga tatcggcaca accttcgtgc
    18481 aaacttgtac cctctccgct ataattaagg gacgcgatac ccatcatgat tccgtgacca
    18541 aattcatggc ctaccacgtc taattctgtc caggggttga ccgattggaa ttggccatag
    18601 tgcacactgt atccatccca aaaagcattc gggttattat tattgtaatg cacaaaattg
    18661 atcattggcg cattattgcc gtcccaactg ctttggttat gaacattttt ccagtaatca
    18721 tatactactt cggctcccca gtgcgcatca agcgcaccat catccgagtt accgttatga
    18781 tattcagcag cggtccattg attgtcgttg tctacgaaat gcgtcgagtc cgcatcattg
    18841 acactgtgat tgagattata ggtgataaca ccattaccgc gggtcagatc tttgagccga
    18901 aactgattgg gcgcgatcga atcggttttg atcgtgcgtg ttcctgcata tcttgtatcg
    18961 gccgttccgg ttgccgttcc atgataaatc cgattaattt ttcgaaccac ttcgcccgag
    19021 gatgcatcca catacaccca atcgcggctc accggtttgg acgcataaat atcaaatttg
    19081 taagctaacc gaaaatccgg gctttcacct tctttggcga tattaacaac aaccgtttct
    19141 gctttcggat aaaagctcgc ctgaggatta tcagtaactt cttttaatat tttttcttca
    19201 tctgcatcct gccatttgta cgcttgggcg ccgatatgtc ctgttgcttt ggatagcgct
    19261 tgcgcctctt ctattttggg tgtaatcgca atgtctgccg gtaaagctgt ttcgccggtg
    19321 acggcataga taccccggtg gtcggagtgc actttgatga tggcatcttc cacgggaact
    19381 gaacggtaca tcatttggaa accatcgtgt gttttaccgt agcggtcggt ttcacggctc
    19441 aacctcttaa acgatatttc caccgatgct ctcagaatac gacgcagaaa ggcttcgttg
    19501 gtctcgcgac cacttttttc gaattcccct gtcaaaaaag aaacccggct ctgtgcatgc
    19561 agcgtcgaag caaaaaataa aatcaccaca ctcgagatta ttatacgtat cgtattgtgc
    19621 ttatgtattg taaaaaaatt catgattatt gccctttcgc caacgttttt gaaaatagaa
    19681 cggataactg ctccagctta gccgccaata cctgcgcaat atctgagcca ttgaggtcgg
    19741 cattgaaccc gctgtctatc aactttattt tttgaagcgt catagaaaaa ttattggttt
    19801 cataataaca caatgcctga accaatatca gatccgccgc cgcaacgccg tcatccaact
    19861 ttagcacaaa atcagcgtcc ttggaaagca cagtatccgc gcgctgtatc gcggaattaa
    19921 aatccgacag cgcccacccc gtaaggctcc agccggcata cgcatcggca tcgttggagg
    19981 cggcaatggt ctggaaataa acgttggcat ttgcgtagtc atgcaacctc accgcactcc
    20041 atccggcacc ggtataaccc gtcaccggtt ctgattcagc gagactcaag aaaatattta
    20101 acgcgtcctc gtaatttccc tcggcgaaag cttgttgagc ttccttagat aaatcttcat
    20161 tgccagtgcc accactacca caagccgtca ttagacaaag caaaatgccc catgttatca
    20221 ttttcataat gatgaacctt ttttatgtag aaactgattt tggatatcac gtacacgata
    20281 ggataaaaag aaaaacagat gagtagaact acatctgaat gtagcctatt acaaacgata
    20341 caacatttga atgtagttca gagattttat gttagccggt aaaactgaga atgaggatat
    20401 aaatcagaac tccaaccata agtaaaagaa acaggtggag aatggccgat aacgaaaggg
    20461 atggaaagaa ttttttcact tggaacacga aagttattta tggtaataaa tactttcgtg
    20521 cagcaaaaaa acactacatg atcgtgtcaa aagatgatca tttattgctt tttttgatgc
    20581 ttgattcgaa taaacctgta tcgctcatgg gcatttttgt taagatttta agcctgtggt
    20641 cataagagct agcgtagcag cacccagtcc agtcacacaa ctagagatta cacctcttga
    20701 caaggtgcgc cgaattgtga gtaatcctat aggcctcaca ggtactgcaa ccgaaaatcc
    20761 gagcatcaag cctttcatga ataactctat atttatgcct ttgtagtttt atttattcgt
    20821 atagcgacca cttatatcat gttcgtacgc aatcccaatc gaagcatgac cataaagctt
    20881 cacgccattt cgacaggtaa tagaacgtct tcgatcacgc taaataattt ttcaagcgaa
    20941 aatggttttt caatagtacg gtcaatgaga ttactgttaa gataactttt catccacata
    21001 tccatttcac cactcgatgc tatgatcttt actttcatat ttcttttgcg gatttcttcg
    21061 attgccagga caccgttcat gatgggcata ttcatatcca taatgatcaa actaaaataa
    21121 tcttcgccat taaactgaac cttatcaact gcatccaatc catgctggct ggtttcaact
    21181 aagtaatctt tttcttcgag cgcctctttt aacatattac gaattgattc ctcgtcttca
    21241 acaacaagaa tacgtttacc ttctccatcc gacgtatttc tcgggaactt ggtgtttttt
    21301 atgttggctt cggcaatcac atcgctgtac ggaagataca ctttaaatac cgttccttta
    21361 cctacggtac tttctacttt tacgaatcca ccatggtttt tagctatgga ctgaaccgtt
    21421 gaaaggccaa gtccggtacc tttatcaggc tccttagtag aaaaaaacgg atcaaaaatt
    21481 cgattaagat tttcttgcga aatgccaacc ccgtcatcgc tgatacttat tacgacaaag
    21541 tgcttaaagc cagatacgta tcgctgcccc gggcaatctt ctttgcgtat agaaataaaa
    21601 agccttcccc cctgcggcat ggcatcacga gcattgatac aaaggttcat aatcaattga
    21661 tgcatttgtg tggagtctgc catgataggg tgcagatcaa actgcatatc caagccaaaa
    21721 tcgatagact taggaaaagt ttcttgtatt atctttactg cttctaaaac aacatcaatt
    21781 agttgaaccg gctttttgtc gctgctttgg cctttggaat aactgagaat ttttttaacg
    21841 agatctgacc cgcgcgctat actcttgctc acaacttcca taacagaagc atcgtgcacg
    21901 gatgcgcgtt cctgatgcaa ggtatcgatc cctatacgaa tcgtcgtcag aatattgtta
    21961 aggtcatggg cgatacctcc tgccagttta cctatcgttt ccactctttg gttttgcaac
    22021 agggtacgtt cggattggcg aagctctgta acatctgaac taaatactag aattgaactg
    22081 ccgtgggtgt ctaggccaac taatagattc cagcgggatt ccacgacgat tttttttccg
    22141 gctttattat aaagcccaat atcacccttc caaaaacctt ttttcagaac actgtgcatg
    22201 atggcatggt aatcatgggt ttggtttcta aacacataat ccaaacacat ctgatcaatg
    22261 atttccgttt cacaccatcc atataattga gcggccgatg gattccataa cattacacgc
    22321 ccattataat ccaatacaaa tacggcatcg gaaacaagat ttaaaataaa tgattgttca
    22381 cgaattttac gctcaaattc tttcaaatcc gtaagctctc gcgaaattcc catcgcgcca
    22441 acaacctcat catggtcatt ccaaagttga gtggccgata aaaaactagg gaatagcatt
    22501 ccatttttac gaacatttat gacttgtccc tcatacttac cactgtgttt catgctctgc
    22561 acaacacggt cgctttggct atcatcggca tatagcattc gaatactttt gcctaatacc
    22621 tcactgcgta agtacccgaa tgttttttct gcggctttgt taaatacaac tatacgccgc
    22681 tgtttgtcaa cggccacgat cacatccaaa gagctttcaa taatgtcgcg ctccatgatt
    22741 ttaggaagtt cagcacgttc tgttgcgttt ttatgtagaa cttcaataat caacgatgac
    22801 aaatgatcat tgttttgatc tgacccgatc cattgaaaat gtctcgacaa gttttcaaat
    22861 tcaccatgct cccctgttac aataatagac gcgtaatcaa aaattctccg gcaatgcgtg
    22921 ataagcccgt acgatgtatt gacgcatacg accgcatccg ggtgaaaata ttgaatacac
    22981 tcaagtgatt cgaggcttgg aattatagat ttatgctcgg cttcaacgcc ttgaatgacg
    23041 agctgtgcca ttactttttc ggcaactcca gtatcatcaa caagaaataa aatccgcgat
    23101 ttgacttgca tacatctttc cggttataca ggagccgcaa gcgtttcagt tttttttata
    23161 ccactggcaa ctgcttggag taataatttc ggaggacatg actttgtcag gaattcgtta
    23221 ataactctcg cacgaatcat ttcatccatc tccccttcca cttctgaata ccctgaatat
    23281 atgatcgtat agaatgctcg ttccggcatg gtttctctaa ttcgtttcaa cagttgcagt
    23341 ccattcatat ccggcatacg atgatcggta agtatcaaac gaatatcatt atttgttgct
    23401 aacagctcca acgcttttaa agggtgaatg caggtgagca cattataatc ttcttcaaaa
    23461 agaatggtaa gggactttag cacttccgca tcatcatcaa cgcataagat cgtgggattc
    23521 atgaattctc cttatctaaa tatattaggt aactaattgt catacatcat ttcttctatc
    23581 atcagctcgc acactttacc atatgatttt gtgtgtaatc gttcctccgc caagctgaag
    23641 gtacatggtt tcaactttgt attgtgcaat ctctttcttt tccagcagcg ccgccaacca
    23701 tccttgattg acatattgtt gttcagacag tacgaccgaa catacaatat caaattggat
    23761 ccggccttct gctttgctaa tcacaatcaa ttcaccctcg gagatataat gtttccatat
    23821 tttttcaccc atttcgagca tttgaagtat atttccccat cgcagtatgt acccgagcaa
    23881 accggaaaga tcgtgggtag ccgtaaaatg gccgatagca taataacctc gttcaagcga
    23941 tacaccacat tgaaaatgca tttgccttgc aatagccacg agaaatccgt tggatataac
    24001 aatcggatac catcgcaacg cagagaacaa cttcaatccg tcctgcggtg tcggaaaaaa
    24061 atcatgaagc gtatattgac ttttgattaa tagtgataac tctgctttgc agcgatcggg
    24121 caactgggtg agtatcgcat gccaattcaa atcatgtttt tgaataaact taaacaaagg
    24181 aataatggct aagcctttga tcatacctcg aatgttaatt tcagattcag aaatcatagt
    24241 ttatttaggc ttagcgcttt agtttagatt tatctatcaa ctgttatttt tttgatgata
    24301 aactcacatc ggtcgatcat caaaatttaa tttcatattt cgcatagact tcacgtccca
    24361 taccgggata cggtgggtga ccaccgttat aagccatgag aaaattatat ttggagttgt
    24421 atatgttata tactccgaca cccaaactct tataactaac ataaatatcc aacatgtgga
    24481 cgggatcata acgtttcgga accagattcc cgttcagatc aatggcatca tatccatagc
    24541 gcgatgaata ccaaacccat gtagtgctaa tacgaacatc ttttttgacc ttaaacagac
    24601 cgttgaacga taacttggat tgcggtacac ccagattagc ttgttcattt actaatttgg
    24661 gattagcatt agtataatct atcacctgat aatttttgac gtcgctcttt ttattggcga
    24721 cataatgtga gaatccaaac gtaatcgcgc tcagatcgga tttgtattgt tgcatgattt
    24781 cgatgccgcg tgttccaaac cgtccgaaat ttcgataatt atccttatta ttttcataaa
    24841 cgtacgccat cggatcggta atcatcacat catatatatt taccgttgtg gatagtgttt
    24901 tattccattg ataaccggtc tcaaactcat aggccgtagt atattcgggt ttgattttac
    24961 cgttgctaaa acgcaaagct tccaagccag gtgcacgaaa agcccgactg tataatgctt
    25021 ttaaatgcaa ttgatccatt actttggtaa gcgctaaacg tggtacagcc accgtcccat
    25081 actcactgtg cttatcaatt cgtatgccca aagtaacatt agctatttca ttacggttat
    25141 aaatttgcgc aaatgcagct gcgccccaat aacgataaga taaatcctga ccagtaatac
    25201 cgtacgatcc attaaaatat tttgcccagt cacgataacc gttagcgccc gccaaaatgt
    25261 tggttttgtt atttaaatcg taagaagcga caatatccat actcaaacga tcgctattgg
    25321 ctttccaatt ggtaaaccat acttcgttat cgagcgtttt attgtttacc caccaaggcg
    25381 tttgatggga atacgtagcc gacggagtta tactccactt ttgaccatga attttatatt
    25441 taattgagcc caagtactcg tgaaagtggt tagtcgtttt tttcggcaat gtgattccgt
    25501 aatgatctct ttcctgtata ttgtattgat ccacaataat ctgaaaactg aaatttccga
    25561 tggatgctcc accgtttaaa aagtatgttt gaatgcctga gttgtttttc atggcgtatg
    25621 atgttccgta ataatcggta taggtacggt cactgcgatt ggcattgcca aaaaaaacat
    25681 gtgcaaatgc gtttacatcc gtatcccagg aatacgcata actgccggac acattagcgc
    25741 gcccatatgt cttcgacatc aatcctccag ctacggttcc attgcctccg ttgaaatctg
    25801 ttgctttttt agtaatgacg ttgatcactg caagttctgc tgtaccgcca tacaacacag
    25861 aacccggacc gcgaataatt tctatccgtt caatctgatt gatatcgtag tgattcccaa
    25921 aataaacatc cgaataaagt cgttctgtca aatcaaatcc atccattagg aatgacacat
    25981 tgccattatc ggctataccg cgcgcactca taatgacagc cccccaagtt gtaacgttaa
    26041 aatcaaaacc gggtaccata cgtaaaacat ccagcaaatc ccgagcaccg gacaccttta
    26101 tttggtcaga agttatgtaa gatataatcc caggagactc tttggctgac agtttatttt
    26161 gcgtagctac gctgacctcg acattaagca actcttctaa cgtcatatca aaataatctt
    26221 tttcctgggc actaagccct gcagttgata cgatcaaggc ggctaaaaaa aaagtgcact
    26281 tcatacctaa tccttagtat ttgaattttt aaaagagtca atcaattatt ttacatagtt
    26341 tcaaaagtga agcttgaaaa ttacgacctt cggattttaa cttttttaaa ctcagtatta
    26401 tttttggttt ttgattttca tcaataacgg acattgatac atagccgctg cggacgaaat
    26461 ccggagtccc ggatatcgtc aatattttta aattattaca agcctcgcta atctctaaaa
    26521 tttgagacgt atacaaatca tcgcataaat aaattatgcc gggcttcatc gtttccaaag
    26581 ctttcagaaa actaacttta tcggaataag taagttgatt gacagcgatc gcattatttt
    26641 tgatgaattt accatcaaca cttttaacca aagcagaacg attggagttt ccgttttttc
    26701 ttgagattac agttatattg gccgattcca tttttgaatc gtaggttaat gcctttagca
    26761 taatactggc gtgcaaagat tcttctattt caacccgaga ctgcgaataa ataggcgtac
    26821 cggttaaaat caagatgaag cagattaata gtttcataaa ttatgtcgtt tgatggatac
    26881 gacgatcagg aatagttata ataaattcgg tgccaacgcc gggttcgctt ctgcactcga
    26941 tggaaccatt atgtttcttt atgatatcat atgagattga aagcccgagt ccggttcctt
    27001 ttccgaccgg tttggtcgta aaaaagggat caaatatttt ttttcggatt gattcggaca
    27061 ttcccggacc attatcttga atacgtataa tcaaattggt attggttacc tctgcattga
    27121 gttttacaac tccatccgtg cgctcgggat tacccgttag aatcgcttgt atggcgttgg
    27181 aaataatatt cataaaaacc tgactcaatt gaccgcggta gcacacataa cttatggacg
    27241 tatcaatgtt attgacgatc aagacttggc ttctattttg acttttaaga atgattaaaa
    27301 caccttccac tatttttgaa atatttgaat attgcatttc tttatgatca atacgtgtga
    27361 agtttaacaa gctgtcaaca atcgtgccta tacgctcact gcacaattca attgccggca
    27421 aaatttcctc ttcgatattt ttacatgttt ctttgatgaa cactaaatca tccgattttg
    27481 cgattttgga taattttctg ctgaacacaa ttccataatt acttatgctg gctttaggtg
    27541 tattgatttc gtgtgcgatc cctgccacca taagaccgat agccgataat ttttcagact
    27601 gtatgagctg atcctgagcc gtttggattt ggccgatact gtctttaagc atcaggtttt
    27661 tttgcgccag ctctttctcg cttcggtaaa ttttataaga cgatttgatc atcatgatca
    27721 atatgagtat caaaatgcac ccaaaaaggg ttccagtaag taaaaatgaa tagttaagtt
    27781 ctttgtgttt ttcttcgatc gagtaggcta tcagcgcttt acctagtact tttccctcca
    27841 attcgtctat aattttttta ctgtaaatta tataatcttc caccaaaaaa agaccctctg
    27901 cagaatcttg cattgtaaga gcaaatcgca aactgtctac cggatgatta ctttttatat
    27961 atctgaaaag atcatggcct gacgcatcat gtaacccgag tgaacgaaaa taaatcgtat
    28021 ctttacttaa gtatttatta aacttttcaa tgctttccgt attctcaagt ttgagagcta
    28081 taggcgcatc ttcttcggaa aggtagtcta tgttttgccg tgcattctct atagcttgtt
    28141 ctcttaccgc actaaggttt tggaagtaac ttgttgtttg ttgcccaaaa aaaagaatac
    28201 tcaaacccaa caataaaccg atgtttccct tgcggtgtct aaagaaatca aacagggcca
    28261 atattttttc gtttgttatc attaaactct taattcctat tgtacgatta tccttatgcg
    28321 gcatgcgtcg gcccctgtat gcatgcatgt tgcttcttca tagcttatat tttgtcggta
    28381 gagttcgcta agaccaagga ttaaaccttt tccaagcgaa cacatttttc tttcggatct
    28441 ataaacaatt tctattcggt caatatcaat gcgatgagtc tccaaagccg gaggcaacgc
    28501 cccgggattc tttctacgga caacattgtg gattgtctta tctatattct caagaacgtc
    28561 aattagtccc cattttcgat caatgaaagc accatacaca gacacaagat taggtaccat
    28621 aaatttccca aacgcttcga gtatggcgtt cacgggttga ccggtcattg aacatgcgga
    28681 agcaactaag gcatataatt cagaatccgg gtatgtttgc gacggcatgt aatgatctac
    28741 ttctatttct gtttgtttca gtaagtttct ccaaccgtct ttacccatat tttgctcgac
    28801 aaattttcgc aactccagaa acacaatccc atgcataccc atctccttat accgtagtgc
    28861 atttcaaacc ttgctttacg gcatgaataa taaacttcga tgaatcggcg cccctgacaa
    28921 acgcatcgaa atcgtttttt gatttttcaa tcattgcgat cttatttttg tcacccgaaa
    28981 cagcatagac gattttataa aaatcgcgct gcgggaactg ctcagatata tgcttaaaga
    29041 actctaaaca gttcatcgtt ttatattcgg catccgcaat aatgactcta attgattgaa
    29101 gctcggataa ggcttcaata gcactcatag gtcccaaagc tgataccaca tggaaatcaa
    29161 cctcaaaaat tcgcactaaa ttattaagaa aattcgggtg gacacctaca aggagtattt
    29221 caggtttatc aatcaattgc ttttcctagt aaaactaatt ctgcaacatt atcaccgaca
    29281 tagtgacaaa caacgtgcca atattaaacc ctttataatc aatctttttt taagatgcgg
    29341 attagtaaat gtttacatat aaacatcaac tgttgatatt tcgacaaaat gattgccttg
    29401 ttagcctcgt gaaagtgctt aacccgatga ctttgtaatt tttacaaata acagttagcc
    29461 tgatctgggg cgaataaaaa ataccctgat gaatgttttt gttgttgatt gatttgaatt
    29521 gagaacgaag aaatgtaatg aaggctatat gtaatagatt ggcttcagaa attcttcatc
    29581 ggttatacac atcaattttg aaccataaag atgtaactgt tgcattataa acctttgtaa
    29641 atgccggcaa atgtcctaca tgatttcaga catcttttgt ttttaaaaca tctcttgtct
    29701 acccttttaa gatttccgca aaacttttat agagtagatt cggtattcta acgtatataa
    29761 cgataatcta actcgattag gtttagacat gatggctttg tttcagcgat cacaaacgaa
    29821 ctattttttc ttacgataga acatggtttc tgcaacctca taatcataac ttacaagaca
    29881 taattcagat tcctttttca gaaatcgcat gacctgcgtt gttccgatca gattatcaaa
    29941 tacgatatta tcagatatta ctgagtacat tccattagtt tgaagtgcaa tagtatcccc
    30001 gagtaaagca tatatagtgt atgtgttatc cctaccaaag gttatagcca gacctatttg
    30061 ttttgcatcc caatatcgca catctatagt gtcccccgtt gctatgtatt ggataatcgt
    30121 atacgccagc gtccaatctc catctaagct atgattatca tgtccgcttt gatcgtcaga
    30181 gcacgaaatg gctattaaga aaataaaact tatgaatttt ataggatttc tcactttgga
    30241 aacgattagg ttactaacac aaggtgtata ttaaaatagt tgcaaaagtt aagccaacaa
    30301 aaccgcatct gtttttattt gttttataca ccgctaatta gttttaaccg ttgaaactgc
    30361 gacaagagtg tcgatatatc gccagcgaat atcaaacaca aaagcgagac atgtttttta
    30421 ttaaacacat gtaatgaagt gaaaattcac actcacatct tttcttaaaa aaactgcaag
    30481 gaaaatctat cttaacgtca tgattagccg atcatattcg cctctgcatt tttaatcttt
    30541 aaacctcgat tcaccatttt cggtaactac cgtccgtaac ctcgtgtgat gtacatagac
    30601 atagcacaat agaaaaatgg acatttaaaa aaagaatacg cagctgatac tttcagacag
    30661 atagtcatac cctcaaacgg taacgcgtcc agccactgat atctattttt attattatat
    30721 gtatttagga gctaagctgg cgatgatcaa tatttagttt tttcattttg gatatgagcg
    30781 ttgatcgatt gatgcccaac agttcggcag cacctccacg accggcaatt ttgccgcgag
    30841 tttttataag tatggttttt atgtggcgta tctccatatc gaccaattgc atttcatttt
    30901 cagttttaga ttgttgttgt gttaaaaata tatgactagg tcgaatagtt tcttcgtcgc
    30961 aaagaattat tgctctttct aatacattcg ataattcacg gatgttgcct ggccatgaat
    31021 attcacgcaa taaggccagt gcgtcgttac aaataaagcg gacatttttt ccaacattca
    31081 aagatagttt atctattaaa tacttagcca gaagcggcag atcgctgata cgctcacgta
    31141 acggaggcac atggatagga aacacattta atctaaaaaa caaatcctca cgaaaagaac
    31201 cgtcacgaac tttggctaat agattctgat gtgttgcggc aatgatcctt acattgacat
    31261 ggatggtttc cgatcctcca acccgttcga attcacgttc ttgaagcacc ctcaaaagtt
    31321 tggactgaac gtctttattg agctctccga tctcatcaag aaaaagagta cctccatcgg
    31381 ctaattcaaa tcgccccttc cgcattgacg tcgcaccggt aaacgctcct ttctcatggc
    31441 caaacaattc actctcaatc aatgaactcg gcaacgccgt acaattgact ttgataaata
    31501 tattttgctg ccttttactg gtgttgtgaa tagtagccgc aataagttct ttgccggtac
    31561 ccgtttcacc tgttaatagt acagttgaac tggtttgact gactttacgg atatcatcaa
    31621 gtaccttttc aaacgccggg cttttaccca ccgcaatatc gcgattataa aaagatttga
    31681 tttccgttat gagtttagtt ttttcatctt tgagttgatt gatctgatat tgcatttgat
    31741 tttgcatcgc cagatctctt ataatgacag taaagtacat ttcattttta tatctgatgc
    31801 acgaaaaatt tacttccgct tccaccacac caccacgacc atcacgtagt ttgaggtgct
    31861 tgtttaaaaa atacaacgac tggaaattgt ttttttgttg catgcattga accatgtatt
    31921 cgtcacaggc ggacctaaaa tcaggagtta aaaatcgctt gaatgaatgc cctatatttt
    31981 ttcccggttc tgatttcaat atttgttctg cagcatcgtt gaatatgagt atacgatagt
    32041 cctcatcaaa tgcgacgatg ccatccatag tcgaatcaag tactgcctgc aatttatttt
    32101 tagtctctga ttctctcata tcatcttcca tgcgctctat ttccaagcgc aatttcttaa
    32161 ggaatattcg ccactcttcc gtctcgccta aatcaatttt ttttgacaaa ataaccccga
    32221 aatgccctaa gacttgacct ttcatgtcaa taataggaat tccataataa aaaccatttg
    32281 gaatatctac cggcacgcta acattcgaat accgcagatc aaatacttgt ggttccggca
    32341 atttatatac ctcctcacaa gcttgacctg ataacatata tgaattagga aatgacccga
    32401 ttcgatcctt tttttcaaat tccaaaacgg ttacgcgctc atacaatgga tctttctttc
    32461 ctatccaaca tatttcaaaa aaaggcaacg cctcttttgc ctcaacacaa agtcgtctaa
    32521 acacttcagc ccctgctaca ccggtagtct tttgaataag agaacgaaat agttctgtga
    32581 aattcacttt tactccttgt gcaaatattt ttacttagta agaccgaaag caaagatata
    32641 aatgtataca caggataagc ctacataatg atgtagagac gactttgtac aacctaaaga
    32701 atttgttaaa cgattttttt gtttacggga gaattgtatg gctattgcca cgtaaaaatc
    32761 tttttgcact cgaaatattt gaatgctgga tatagtatcg gcagcaatgg tatgataaca
    32821 aattgaaaga atgttcaagt taacgcgtat gcttctcggt aattatcata ttactcaata
    32881 tcttttacag gtaaagacat tagcgtaaat actatgaaat gctatcgaga ttaaactgaa
    32941 taattaaacg ctagttataa ttatgaatcc atatcattac ttgggatgat tcttacataa
    33001 tgccctcgat ttcaaaaatc tttgcctaat ttagaatacc gttatctact cttgggtata
    33061 tcggaaagga tttggccaaa ctcaaatctg accacggata aacttggaga cgagttcgcc
    33121 tttggagttg acgtgcaatt ttttgtagat atttttgatg tgaaaacgga tcgcatcgat
    33181 cgttacattc atctgggagg caatcagttt ataactaagc ccatccacca aaccctgaac
    33241 aacctgacgt tctttggccg tcagtacgtc gtcctctttg ggcttgggac tgaaatactg
    33301 aattacttta cgtgcgattt gcggtgacat cggcgctcca ccggcgttca gttcggtcaa
    33361 cgcatcccgc aattcgacca agggcgtgtt ttttaaaaga taaccggaag cgccggcaca
    33421 taaagattga aaaatttttt cttcgtcatg atatacggta agcatcgcga tatcaatctc
    33481 aggccatttt ctttttaata tacgaatacc gtcgatgccg ctcataccag gtaactgaat
    33541 atccatcaaa acaatatccg ggcgcgggcc atcttccaga cgtgccaaaa aaagttctgc
    33601 cgtcggatca aaagataata ccttaaatcc gggctgactg ctcagatatc gggtaatgcc
    33661 gttgcgaata tcgctgtcgt cttcgactat ggagacgctg atcataggta tttctctttc
    33721 actaatcact ctattatact cctatactta ttgataatca atcacgtatt ggtatctcta
    33781 cgcgtatcgt aacgccttga tctgaagagg attgaaacaa tcctcccaaa gtttcagctc
    33841 gcattttcat atttttcatt ccgtgcccgc ccatcgtgac cccgcccttg aggccgccgc
    33901 cattatcacg cacctccaaa tatattcgtt tatttttcag cacaagtcgg ataactactt
    33961 ccgtcgcttc agcatacttg tatatattat tgacagcttc tttaaatatc agatacacgt
    34021 tctgcttcac cagcacaggt aaagtgatgt cttcatctat accttcactt tcaaatttta
    34081 cggtaatatt ttttcgatcc agcaaatcat gcgcaaaatt ctgcattcgc aaattcaaat
    34141 ccgttacccg atcattgcga gcatcaatag accatacgat gtcgctcata ctggatatca
    34201 cattcctgct caagtcgccg atcgttttca acatcggctt tgcttctttc ggatcttctg
    34261 tttcctgcac taattctgtg tacattgcga tcttggaaag attggatcct atatcatcat
    34321 gcaaatcgct ggcgatttta acacgcagac gctccacggc taaaagtcga tggacccgat
    34381 acaaatacac gcgccacaga ataaatgcaa taactgaaag caccatgacg cgaaaccacc
    34441 acgtccgata ccacggagga agaacccgaa aacgatagga cgtttctcca ccgacgacac
    34501 catatatatt tttggctcgt acccgaaacg tgtattcacc ttcattcagg ttggtgtaga
    34561 tcgcacggct ttcttcactc cagtccgtcc attcgt
//
LOCUS       DAOQGF010000024        34472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_560_length_34472_cov_6.686056, whole genome shotgun sequence.
ACCESSION   DAOQGF010000024 DAOQGF010000000
VERSION     DAOQGF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_34472_cov_6.686056"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PK937_05400"
     CDS             <1..658
                     /locus_tag="PK937_05400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HNH32037.1"
                     /translation="ARAMKNFGMKDLRLVRPCDHLDREALWMAVKAEDILHSAQIYST
                     LTDALKGIQFSVATSNRRRETHFPSFTPREIAQQMAAFSPITSCALVFGGEKNGLSTE
                     DVHACRVLSTVTTAPVQNSLNLSHAVMIYLYEVFAARLTGNPQFVWDMAKPADTEILY
                     ERIQRVLEAVKFKPRKTNEGFLLGVRRILGRTPLEDRDVRLLHKIFQEIERYVKRSEA
                     "
     gene            701..1486
                     /locus_tag="PK937_05405"
     CDS             701..1486
                     /locus_tag="PK937_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNH32038.1"
                     /translation="MEFEFISLLFISVCGFAFLAGFVDAIVGGGGLVTVPFLFVGFPS
                     APVSMVIGTNRFSSIPGTIVAARHYLRHTQIPVKTLLLCAFSAAVMSYTGAAVSRLLK
                     PDVLKPLMLIIMVAVAAYTYMKKNLGHTDDHTFSDWRLPATAILVCAVLGFYNGFFGP
                     GTGSLLVFAFVSVIGFSFLKSSAMAKIVNVVADISSLIFFITNGFVFWKLAIPMAFCQ
                     IAGSVVGSRMAVLKGNRFIRYVFLVVVGGLILRFGYDVWKMMA"
     gene            1996..3138
                     /locus_tag="PK937_05410"
     CDS             1996..3138
                     /locus_tag="PK937_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32039.1"
                     /translation="MKKFPLLMVFVMIASCTDHQQMDNASLEDVEIRETLNEMAQTLA
                     SDPSWIDQTLIEKLKKGAVKSADLPSSIKLSKSNGFVQDIEIEMYDNSNVKSLSKSLG
                     TTPYYVGLDPDVYNFSPSIQEELTGLPVMDMPVFYFDGQSVSETTVQLDPDKQTDKLI
                     FFIKSTSLEPHTLSKSMQIAGTFFACRSIKVKVKQDESDEEFEVYFSNGSHPINSYFN
                     GTTDHVFNGNTREDAAGNSRYYMDVNDKNLWYPTSDGQEISLIRVDNLTDSVRMVAIE
                     ADWSSGNYVQGRYNRNQQVGTTSEILTDYYDMTDNTKKLQENSAYYVDLKTSLNDDRY
                     QKGAIKSINARTLYARTNNGEHYIETANADGGALTDVDWIIKQIVQ"
     gene            3204..4145
                     /locus_tag="PK937_05415"
     CDS             3204..4145
                     /locus_tag="PK937_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32040.1"
                     /translation="MNKFVLAFISIVLCGCSGCTDDKKKPYEGFLDKDREFVIAHVAP
                     DEDRINPVEVFVGRLLTIVPDSTDGANIQDYLLKIQLRQFQNEYYALLKYHDDAQVFI
                     TSSGQTVELQNVGKGTYRDVSNALHISALQTCTLKVTRPGGKVYQAMVRVPDHITITS
                     LSPGDTTVLYPKKNTPESDFCGAVAYISGPAVTGAYIYRSKFDETAHLQSSNNSGSAV
                     IIELCGGVEYTNISETVMAFDTVLTRAWGMGEDTGFGYDLFDSLLHWRSAATPITYRS
                     NITNQDGAKIAGYFGAYNSINTHFVVKSLRDSCQCNN"
     gene            complement(4581..5282)
                     /gene="purQ"
                     /locus_tag="PK937_05420"
     CDS             complement(4581..5282)
                     /gene="purQ"
                     /locus_tag="PK937_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNH32041.1"
                     /translation="MKTGVVVFPGSNCDHDAFYTAGELIGHKAEYIWHKDRKDLRSFD
                     LIIVPGGFSYGDYLRTGAIARFAPIMQDVMDFAKYGGTVFGVCNGFQILTEAGLLPGA
                     LLRNSHLHYVCKNIYLRAENDNSRFTNTLKKGEVLMVPIAHGEGNYFADNETLKRLEG
                     DNRVLFRYCTENGTINADGNPNGSLNNIAGIMNENGNVAGMMPHPERCADPALGQNDG
                     LKVFESLLIARQKAA"
     gene            5403..6731
                     /locus_tag="PK937_05425"
     CDS             5403..6731
                     /locus_tag="PK937_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006667922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH32042.1"
                     /translation="MSRHPALTPSEKKSIWGWCMYDWANSAFILTVVTAVLPVYFADV
                     VVPKEGFDIGGTLYSATSLWGFMISSSALFVFLCAPMLGAIADFTAAKKKFLMVFCYT
                     GSLFTVLLFFCGSGDVWMTMIFLIIAQIGFVGGNIFYDAFLPHVAPPGQMDWVSGKGF
                     AYGYIGSDIQFLLCLGLILGHDALGLTGTMAVRLVLAFSGLWWAGFSIFTWKYVHEAP
                     ATENIPDDLRSSPKVIAYIRLGLRRTVGVLKKIVSTPYLLIFLIAFMLYNEGIQTTIV
                     MATIYGKEELGLSTSVLMITLFVIQIVAMFGALLFSKLAERWSTQKAILTTLVGWSLV
                     VLYAYGMTTSAEYMALGVVVGIVMGGSQALSRSYYGAMIPKESSAEYYAFYSVFTKFS
                     AIWGPLVFAVIRQATGSSRLSIVSLIVFFILGMILLTMVRVPKSAEVPAS"
     gene            6739..7983
                     /locus_tag="PK937_05430"
     CDS             6739..7983
                     /locus_tag="PK937_05430"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH32043.1"
                     /translation="MKRSPLTVIFITVFIDLVSFGIVIPLLPFYAQQFDASGTTIGLL
                     LSVFSLMSFLFMPLWGRISDRYGRRPVLLVTIFCSVIGYIIFANAFALWLLFLARIMS
                     GIGNANISVAQAYISDVTSAKERAKGMGLIGAAFGLGFVFGPLIGGVFSGEAFGAYRF
                     AIPGWIAVGLSLINFFMAYFMLPESLAQTKQDEIKAHHLIDVRGLQRAIKKENLGLLM
                     IFFFIVTIAFSSIYATFPLFIMEEPFRLTSSAMGWFFVEIGIVSVIIQGGLIGRLNHR
                     FGEQRLISFGAIAMAIGFITFPLSAYLPIGGIVLLIISTALISMGSSCLAPTTMGLTS
                     RLADVSEQGSILGIAQSFASLGRMIGPFIGGAAYDYAGHKSPYYFAFVLMAVAAVMAF
                     ILKSRDLKLDEAPVAMEPVAEV"
     gene            8069..10642
                     /gene="topA"
                     /locus_tag="PK937_05435"
     CDS             8069..10642
                     /gene="topA"
                     /locus_tag="PK937_05435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNH32044.1"
                     /translation="MAVSSGKKKTTKTVKPKAVKSDTAEKPVKEKSVKKTKTPVAEEK
                     TAVESAPAKRTRGGRKPFVRAKTVKEDIEITIPDNIERTNKSLVIVESPSKAKTINKY
                     LGKSFHVEASVGHIKNLPSNKLGIDIENGFTPEYRIIDGKQDVIRKLKTLAASTKNVF
                     IATDPDREGEAIAWHIAGEIEKHNPSISRVLFNEITKSGVEEAMRTPTKIDPKLVQSQ
                     QARRVLDRIVGYGVSRYLWGVVRRGTSAGRVQSVALQLVCKREEEINAFVPQEYWSIH
                     AKVQDEPTEAFLAHLHSIDGKKVDIASGDVAHRFVEDIRKKNFLIKDIRKREARRNPS
                     APFVTSSLQQEASRKLGFSPKLTMMLAQQLYEGIDLGSEGLTGLITYMRTDSTRVADT
                     ALVSVREYIMGSYGREYIPHTPNQFKSKKANVQDAHEAIRPTNVKFTPKTVNKYLTPQ
                     QAKLYELIWNRFVASQMTPAELDQTTIDISADEYLFRTTGSVVTFKGFLQVYEEGRDE
                     GDKTQDNGEEEDNSGLLPANLKINDVLKLHELLPEQHFTKPPARFTESSLIKELDNIG
                     IGRPSTYAMIVGTIVEREYAELRERKLYATDLGKSVNKVLNDHFNALFNVQFTAEMEE
                     ELDKIESGELGYKKVLDDFYNPFVKNMKDVESKKDAIKESVQEKTDETCEICGKPMVI
                     KWGRFGKFMSCTGYPECKNIKKVSKDGEAAPPPEVTDEKCPNCGSPMVVRQSRGGKFL
                     GCSNYPTCKTAKAHPDDIIKDVICPKDGGQLIRRRSRYGKFFYGCANYPNCDFVSWNE
                     PVATRCPHCDAAFMVKKTSKRKGDFLLCMTCNHEEILPADTSVMKEQPENESVSFNEG
                     E"
     gene            10683..11222
                     /locus_tag="PK937_05440"
     CDS             10683..11222
                     /locus_tag="PK937_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HNH32045.1"
                     /translation="MADEKEQKDELLEKGAALSTAIIGVFLSISSILGGNANDDIMIH
                     RGLANNQWSYFQAKSTKQALYEVNRKMFETQLADPSLAESYKKEIQAKLEKFDSEVAR
                     YDTEKKEIKAKAEEHEKLSDQAAEIEDKYDYAEGFYQIAIILAAVAMIAGNRKLWFFS
                     IVLGSIAIAITGFAFFVSL"
     gene            11445..12497
                     /gene="asd"
                     /locus_tag="PK937_05445"
     CDS             11445..12497
                     /gene="asd"
                     /locus_tag="PK937_05445"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNH32046.1"
                     /translation="MKKIKVGILGATGTVGQRFIELLHAHPYFEITALAASERSVGKK
                     YNDLNTWKLDSVLPSRIGEMIVQPCSPGMDARVMFSALDSSVAGAIEIEFARAGYIVV
                     SNAKNYRMDEDVPLLFPEVNADHTRLIEIQKKNRGFDRGYIVTNSNCAIMGFVPIVHV
                     LHQHYGVVSVDVTTLQAISGAGYPGVPSMDILGNVIPYISGEEEKIETEPLKIWGQML
                     QDRIQFASIAISASVHRVPVLDGHLASVTAKLKQLPAIGDIKQKLSEFKSVPQELNLP
                     LAPMRPIVVIEGDDRPQPRRDVTLEKSMAASVGRIRYDRSGNLKFSCLVHNTVRGAAG
                     GAVLNAELLHAKGLLE"
     regulatory      12793..12926
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12992..14197
                     /locus_tag="PK937_05450"
     CDS             12992..14197
                     /locus_tag="PK937_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNH32047.1"
                     /translation="MKFGTSAIHAGVNKDTAYNSVTTPIYTSSTFRFEEPGLTKGYDY
                     TRSGNPTRSALEENIAALEGGICARAVATGMAAVATALHFFKSGDHILCTHDCYGGTE
                     RLLRTYSELFGLRVTYVDMCDLKSVEAAWLPETRGIWIETPSNPLLNIVDIRAITAIA
                     RARRGLSIVDNTFLSPYFQRPFELGADIIVHSTTKYLNGHSDVVGGAVVVNDSNLADR
                     LKFLVNALGLGAAPFDCWLVLRGIKTLVPRMQAHERNALAVARFLSEHPQVRRVFYPG
                     LPSHPNHALARCQQYGFGGMVSFELDGDSESMRRLLKGTKLFTLAESLGGVESLIEHP
                     ATMSHASMDAELREEAGINDRIVRLSVGIEETSDLIEDLASALRQCQYNTSATPASAR
                     SVFSSINAH"
     gene            14230..15576
                     /locus_tag="PK937_05455"
     CDS             14230..15576
                     /locus_tag="PK937_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNH32048.1"
                     /translation="MATEEKKSTYRFETLQLHAGQKPDSATNARAVPIYQSTSYVFNN
                     ADHAAALFGLQEFGNIYTRIMNPTTAVFEERIAALEGGVAAVATSSGQAAQFLAITTI
                     AQAGDNIVATSLLYGGTYNQFKVTLPRLGIDVKFVNGDRPEDFERLIDTKTKALYIET
                     IGNPRLNIPDFQAIADVAHRHGIPLIVDNTFGAGGYIAQPIRFGADIVVHSATKWIGG
                     HGTAIGGVIVDAGTFDWGNGKFPLFTEPSPGYHGLKFWDIFGSAGPFGNIAFAIRARV
                     EGLRDIGPSQSPFNAFLLLQGLETLSLRVERHAQNALALAHWLKKQPQVSWVSYPGFE
                     DHPYHANAKKYLRPGHYGGVLVFGIKGGKEAGKKFINSLSLASLLANVGDAKTLVIHP
                     NSTTHQQLSDAEQHTSGVTPDLIRVSVGIEHIDDIIADFEQALHKTESLTVEESAA"
     gene            15573..16700
                     /gene="metX"
                     /locus_tag="PK937_05460"
     CDS             15573..16700
                     /gene="metX"
                     /locus_tag="PK937_05460"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNH32049.1"
                     /translation="MNLLVSSMPSVFSTVKYTTVFDTIPLRLQQGGTLSNITVAYETY
                     GTLNNEKSNAVWVCHALTGNAHAYSDGTNVPGWWDGLIGKNLALDPDEYFIICSNILG
                     SCYGTTGPASHYPADGLPWRKRFPLITVRDMVQVQHALLEHLGIERLQAVIGGSLGGM
                     QVLEWALMYPEKVARIIPIATAASHSAWAIAWNEAARRAIVQDPAFQNGGYNEQPVKG
                     LGLARWIAMISYRSQKSYQDKFARGGQPCDQINPDFFYSDETAQPFAIEHYLDYQGKK
                     LTARFDALSYIRITQAMDRHDVTEGRGSLDYVLRSIRAKSLCIGIRSDVLYPAEEQKA
                     IARGIKNARYIELDSDDGHDAFLIKNELMNETIAKFLKENE"
     gene            16697..17449
                     /locus_tag="PK937_05465"
     CDS             16697..17449
                     /locus_tag="PK937_05465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNH32050.1"
                     /translation="MISLKDKVALITGGSRGIGAATAILLAEAGADIAINYLNSEANA
                     RRVVDRIQALGRKSAAIRADVSDDAQAKELVQSVILKFGRLDILVNNAGIWTRAPIGL
                     TDKSVWDATYLTNVQSYLSVTNAAVPHLMKSRGRIINITSTAGQRGEAFHSHYAASKG
                     AITAFTKSIAVELGPQGILVNNVAPGWVDNDLNREVFSDAGFKKTVIESIPIRRIASS
                     EDVAGAVLFLASDLSRHITGSTISVNGGAVLV"
     gene            17477..18853
                     /locus_tag="PK937_05470"
     CDS             17477..18853
                     /locus_tag="PK937_05470"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNH32051.1"
                     /translation="MIVMKFGGTSVQDAGAIRRVAEIVRRHAERKVLVVVSACAGVTD
                     QLVRLSQAIERRDEAAIGKIKETLIDKHKMEAQTLTLSAEEKEQLERFNGEFERCVNG
                     ASILHEVTPRTHDRLLSFGELWSSVLTLSAIRQTGQSISWLDARTVITTDNAYNAAHP
                     QTDALPLQVQQKIIPALERGIVITQGFLGATRDGHTTTLGRGGSDYSASLLGASLKAD
                     EIQIWTDVDGILTTDPRLVHVARQLTHVSFDEATELSQFGAKVLHPKTVLPAIQKNIP
                     IRVLNTFNPSNAGTCITREAIKGKGIVRSIAFKRGLEVMTVRFNDPEQKSPILAEIFA
                     RLQEAGTDVELVNTTERGIVLAAASNERWRVFCDHLPASWHITFQTGRALCALVGHLI
                     AEDFSNLTRALSVMQSEAIHVEAIAQGGSGHHWAVLLREHQLTPAVRLLHADFFENRA
                     SGQTVAVA"
     gene            18938..20743
                     /locus_tag="PK937_05475"
     CDS             18938..20743
                     /locus_tag="PK937_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH32052.1"
                     /translation="MKNAGFFLWVWVFSISLCVKELKAGGDEYWFLKNQAHTTPENKL
                     YDTPDWRIALDGPIRGSAAFSGGHVYIGTAKGTLYAVREKDGTVEWNRNFGSAIHSTP
                     AVKGNHLYFTDNSQHLYCVSAGDGKEKWRYAFDKALPYDWKFDYWYSSPVFWGENIIV
                     GGDDGYLHSVRLRDGKAQWKYKAESVIRSTPVIRDGIIYFGDCMGYVYAVDAATGKSK
                     WRSETEGVRIDQSQYIFDRKAVISSAALSEDVCVIGSRDGFLYGFNTIDGKQLWSVDY
                     KISWVNSTAAIREGIAVTATSDGRYVNAVEAKTGKEIWRFNTRQILWSSPLIINNHVY
                     AQSCDGVMHILNFKTGEKISEHRTITPIQSFSSPVFTGKTLLYTNDSGILYASFGRPT
                     PVESRTRKLALWMDKVGLQFGNNMDVRLKNYLNDIGYEAIDTNRFAEILTQSDSAPKT
                     IVVVISAAFPDTILQGAKNSLVRRFLDQGGAIINFGFNPTVFDPRIETYNFQKAEEIF
                     GVAYGPNDLRSFQGLYPSFATELGKKYQLPDFWTGFAGVNKNQVDEVLGLNENGLASS
                     WIKYYGANKKGAWIQIAMDRSNPVDYNFLKYFNAK"
     gene            20915..22387
                     /gene="carB"
                     /locus_tag="PK937_05480"
     CDS             20915..22387
                     /gene="carB"
                     /locus_tag="PK937_05480"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNH32053.1"
                     /translation="MPKRTDIKSILVIGSGPIVIGQSCEFDYSGTQSCRALKEEGYRV
                     ILINSNPATIMTDPETADRIYIEPITPAMVEKIIERERPDALLPTMGGQTALNTSVEL
                     FKSGVLEKYNVEMIGAKYDSIQTAEDRELFKEAMERIGLHVPKGRFVRTVQDANEAVS
                     EFDYPIILRPSFTLGGTGGAVAYNIDEYNDLIVKALAASPISEVLVEESVIGWKEYEL
                     EVMRDKNDNVVIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQKMRDAALKIIRKIG
                     VETGGSNIQFAINPANGDMIVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     IPNDITKKTPASFEPTIDYVVTKIPRWDFEKFQGANTTLNVQMKSVGEAMAIGRNFKE
                     SLQKALRSLENGRFGLGADGKDNPPADASDETAVEAYRQEVINKLRVPNSQRMFAIRD
                     GFKMGMTVEDIHRYTFIDPWFLNNIREIIELEGSLRAALA"
     gene            22465..25854
                     /locus_tag="PK937_05485"
     CDS             22465..25854
                     /locus_tag="PK937_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32054.1"
                     /translation="MKALALIAWLFVLSFPVFGQIEISFNHVTTKHGLSEGSVHTILQ
                     DRRGFLWFGTQDGLNRYDGYTFAIYRQGSPRQALSDNFIYTLCEDTANGIWIGTFNQG
                     LCRFDRMRESFTIYKHDSTDATSLGDNTIRALYRDRKGILWIGTFRGGLDQMTADGKF
                     KHYRHNPHDPAGLSSDNVWALYEDSRGNFWVGTDGGGLNLLDPMTGKCTVFKNEPGNI
                     RSLSHNTVRAIYEDRAGVLWVATSGGGLNRFDYVTRTFTTFKNHRNDPQSLSHDFVRA
                     LYEDKRGNFWVGTLNGGLNLMDRVTGKFIRFLPDVTDPGSIIHKSVISIVEDRSGTVW
                     FGTWGGGVSYISPTGHRFINYRYDPFNSYSLSQDRIYALHEDQSRTIWVGTFGGGLNR
                     YLPDQKKFLHFQKPQHGLSHDDIWSIAEDQNGDLWVGTNEGGLNRWDRKRGTFEVFRN
                     DPHDANSLSNDNVFTVYVDPLNTVWIGTSGGGLNRFDRNARTFRRFTHDPALGYTVSS
                     NQIRALHQDKEGLLWVGTFGGGISVWDRKKEIFTRHLSSNYDSSSLSSDLVLRIFESR
                     DGLIWIGTAGGGLNRYDKQTGHWKTYTDKHGLANNVINGILEDQTGRLWISTNKGLSR
                     LDPKTETFRNFDEGDGLISAEFGVGAALRAHDGRMYFGSNRGLVTFHPDSIKENRYVP
                     PVIIAGFKKFNKPFVLERSVSETDTIIISYKDNFIAFEFAALNYTHSEKNLYRYKLEG
                     FDRDWIDAGSRREATYTNLDGGTYVFRVKASNNDNVWNEEGRAITLIVEPPFWETVWF
                     QFLVGAFLCAVLVYRFRQIQNQKRELERQVQVRTHELEKAMDSLKRAQVQILQSEKIG
                     SLAHMVAGIAHEINNPLTFVYSNIQLLRARVQRLFLFMGFLDKRSHTESWTDDEKKQY
                     EKFKHTLDYDYLEKDLDDMFRSSVEGSTRIIKIVNSLRAFSSLDQEEIQETNLLESLN
                     MVVDLFMRQHKDIVIERKTTEIPFITVHLTLMHQAFINILDNSVKAIRDAEKQNLLAS
                     GDGRITLSAEWQSDKNKIRLCFIDNGVGIDSSVRNKIFDPFFTTRPIGAGAGLGLSEV
                     FTTVRKHGGDVTVESEPGIGTTLCIDLPVKTLFHENYQHGS"
     gene            25854..29024
                     /locus_tag="PK937_05490"
     CDS             25854..29024
                     /locus_tag="PK937_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32055.1"
                     /translation="MKSTIMYSLLCLIYFSPLAAQDEQGEPFIRNYFPQEYRAQSQNW
                     SIVQDHRGLMYFGNNDGVLEFDGIRWRLIPAPNRTSIRALAVDEVGTIYVGAVGEFGI
                     LKTDSTGIRQYYSLSASLDSSFKGITDIWEISCIADKIYFRSNKYFFVFRRSADGSYH
                     FVKAIISDTQFHRSYLIHNTIFVRQDKRGLYIIDQDTLHLLPYGKEFANLRIYGMLPF
                     KDGRFLIATREKNLFVATRSGFAAMKTSVDDWLIQHQLYYASKLKNGNMVLGTILGGV
                     VELSEDGSFIRRVDKSSGLLDDNIWATYVDHNGILWLAMNNGIARVEIGSPYSRFSEL
                     HGLRGTVLSLTRWNNTLYVGTSRGVYRLNRYPSSPEKDVPLSSSQEIWKFEPIAGITA
                     QCWSFVDNEVSLFAGTAQGVFRINGQQAQMVDNRSVRNMMRSKRNPNQVWVGLSDGLA
                     LCRIDKEQWVRVEEHRTIKEEVRTIAESLNGDLWLGTRFKGVIHLKREGDSLNWPVTR
                     YDRANGLPIGEMYMYEVDGRPLVGTTSGLFRYDVDHNHFVRETLFGPLLSDSATVVHH
                     FLQHPQFGLWMTAQVGERFEILHAASSSRAISYTVRNPFLRISDFRVNIPYPESGNII
                     WFGGADGLIRYDGNIQYAWDSQFYALIRKAVVMNDQRQNIPILSDTSQHVMLAHYDNN
                     IRFEFSASSYERDGSNQFQYYLEGFDPGWSEWSTEFRKDYTNLPDGVFRFRVRARNLY
                     EQVSVEDQLRFVIRSPWFKSWWAFGFYTALVFLCIYVIIHYRERRLVLANDNLERIIQ
                     QRTAELKQKNVELENLNEKKNEYLGIVAHDLRSPLTTIMGYIDLLIADAETDTFKPKE
                     AVKDLVRISRISHQMSHFIRELLDISAIESGKIRLEVLPDNLGTIIRDAEPLHRRAAN
                     QKNIAMDYEYDPTLPDTLMDREKIKSVVDNLINNAIKYTYPGGKVRVYHEVRDQDVVT
                     HVEDTGQGLSDEDKQTVFMSFKRLSAKPTAGESSTGLGLAIVKKIIEIHNGQTLVRSN
                     KGQGSTFSFSLPRADKQSDKNLKVKV"
     gene            29030..32524
                     /locus_tag="PK937_05495"
     CDS             29030..32524
                     /locus_tag="PK937_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32056.1"
                     /translation="MQYQKFFRSIGVMLVFVGMGAPHDVFSQQINMKFEHLSGDQGLS
                     QSTVGVILQDHTGLMWFGSRNGLNQYDGYRFTVYKSSPTDPESISDNWITALYEDRNH
                     NFWVGTLNKGLNRFDRARKKFKHMISDPENQRTISHNSITAIMEDRHGNIWVGTKNGL
                     NVIPYDSMRTETTMRFVRYFSSSQVVNSISHSWITAITDDENGDIWIGTQQGLNKITN
                     GEIIRYFADKQGGSGLPSNNITALFKDANNNIWIGTEDGGLCKLDRKNTQNPVFEKIP
                     VHSGTDASTTHADIVTVMVDRQNIVWVGTRLGLDRIDFNSNTKLFFRHNPNDVSSLSD
                     NEIRALCEDRSGNLWIGTYGGGGLNKLDLKPVKFNHIRHKPDNPNSLINNIVRSFWED
                     SDGILWIGTHDGLNRYDAGTDRFTAYRAGPGRLSSNEVRTIAEDKNGMIWLGTEGGGL
                     NKFDRRTNRFTVYQHRPGDSLSIAGNDISVLLSDKYNQLWIGTFGDGLDKLIVENGKE
                     IFVHYRNVLHRSQSLSHNEVSSILMDSEEKIWVGTFGGGLNRLDSVQDRTGYASFKRI
                     AGRGESNSSLGSDVIFAISEDSDKNIWIGASSGLNRIVRTTDGSLKFYNYYEKDGLPD
                     NYVYAILPDEHGNLWISTVQGLSRARLIQDENDQSEKLMFRNFDESDGLQNNEFKYGS
                     YYKSRSGEIFFGGTNGFNRFYPKLAMDNPHIPQIVLTAFKTMHKSDKAFVDAYIESVY
                     RHDPIILDYNEDFSFEFASLDFTEPAKNQYAYKLEGFDKNWILAGTRRYVNYTNLDAG
                     NYVFNVRGSNNDGVWNEQGISIPIRIVPPFWKTWWFIALSFSALMGLLWAFIRYRVRV
                     IELQKNKLEVEVSNRTREIKAQRDDLKAINSQLEKTLQELNETQSQLMHAEKMASLGQ
                     MVAGIAHEINNPLTVVDGNLHLLEENVLRWDHIITEYERLIETADPEKIKMGMMEIRE
                     RHEYEYVRQETMKILDSCKNGSQRIKHIVQDLRKFSRTDAMGFIPADLHEGIESTLTV
                     LKSMLSDRITVHKQFGTIPMLDCYPGLLNQVFLNILTNAIEAIPNEGIITIKTYLSEN
                     NIRIHIKDNGIGMHPSMVKSIFDPFFTTKEIGKGMGLGLSISYGIIKKHNGDIEVISE
                     EGKGSEFIISLPVIKRVS"
     gene            32524..>34472
                     /locus_tag="PK937_05500"
     CDS             32524..>34472
                     /locus_tag="PK937_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32057.1"
                     /translation="MKKHIAQLIVWICWSGTIVLAQSDRIKFERLSIGEGLSQSKVFS
                     IYQDHKGFLWIGTMDGLNRYDGYGFTVFRHDPRDSTTISENYVWSIIEDKEENLWIGT
                     NGGGLNRFDRKTQKFKPYRNIADDPHSISDNRVWALYVDRDGVLWVGTRYAGLCKYDR
                     ATDRFIAYKNNPQDSTSLSHNYVMTVFEDSKNNFWIGTNGGGLNRMDRATGKFTRYRY
                     KPGQRSPVPEERIWALSEDREGYLWVGTNNNGLSRWNVATGEFEIFRHSSADPNSIAD
                     NWIKSIYFDRQDRLWICTNGGVSIYDKTTQKFYNYRNNPADPSSISNNFVNAFCEDRM
                     GILWFGTYGGGVNMYDPYKERFKHYHLDLSQPQGLSGNIVISFAEDHEGIIWIGTDDA
                     GLNRYDPKKQTYRHYKHNPADAKSISNNRVSVIYEDRKHNLWVGTRGGGLSRFDRKTE
                     QFKNYRIDPISSAQSNNVVFAILEDTSGTLWVGTRAIGLQIFDEKKGEFRRVRYDGRN
                     PADNGVQVLMQDSRGYIWVGTSGGGIVRLDPKTYAFTRFVHDPSNPNSLSHNAIVSIL
                     EDAQGKIWVGTSGGLNRIDPSNDKILQITEKHGLPNDVIASIQQDGNGQLWVSTNKGL
                     SRIDINNFENGGPTAHNRIRNFDVF"
BASE COUNT         9315 a         7126 c         8529 g         9502 t
ORIGIN      
        1 cgctcgcgcc atgaaaaatt ttggcatgaa ggatttgcgt ttggtgcgtc catgcgatca
       61 tttggatcgt gaagccttgt ggatggctgt caaagcggag gacatacttc attcggcgca
      121 gatctattcg acacttacgg atgctttgaa aggtattcag ttttctgtgg ccacaagtaa
      181 tcgccgtcgt gaaacgcatt tcccgagttt tacgccgcgc gaaatcgcgc aacaaatggc
      241 ggccttttct ccgataactt catgtgcctt ggttttcggt ggagaaaaaa atggtttgtc
      301 aaccgaagat gtgcatgcgt gccgagtgct ctccaccgtt acgaccgctc cagttcaaaa
      361 ctcgctcaat ctttcgcatg ccgttatgat ctatttgtat gaagtgtttg ctgcgcgact
      421 tacgggcaat ccgcaatttg tgtgggatat ggccaaaccg gcagatacgg aaatattata
      481 tgaaagaata caacgcgtac tggaagccgt aaaatttaaa ccacgaaaaa ccaatgaagg
      541 ttttttactg ggtgtccggc gcatattggg gcgtacaccg ttggaagacc gtgacgtgcg
      601 tttgctacac aaaatatttc aggagatcga acgctacgtc aagcggtcgg aagcgtaggc
      661 ttcttttatc atattacatt atcaaactta gagattattc atggaatttg aattcatttc
      721 attactattt ataagtgttt gcgggtttgc ttttttggcc ggatttgtgg atgcgatcgt
      781 cggcggcggg ggactggtta cagtaccgtt tttatttgta ggatttccgt cggctccggt
      841 ttcgatggtg atcggtacca accgcttttc ttcgattccc ggaaccatcg tagcggcgcg
      901 acattatctg cgtcacaccc aaattcctgt taaaacgctc ttattatgtg cgttttcggc
      961 cgcggtgatg tcctacacag gcgccgcggt ttcgcgatta ctcaagcccg acgtgctcaa
     1021 gccactgatg ctcataatca tggtagccgt agcagcctat acatatatga aaaaaaatct
     1081 gggtcatacc gatgatcata cgttttcaga ttggcggtta ccggctacag cgatactcgt
     1141 gtgtgccgtg ctcggctttt ataacggttt ttttggaccg ggtacgggca gcctgctcgt
     1201 atttgcgttt gtttcggtta tcggctttag ttttttgaaa tcatccgcaa tggccaaaat
     1261 tgtcaacgtc gtcgccgaca tctcatcgct gatatttttt attacgaacg ggtttgtgtt
     1321 ctggaagttg gccataccga tggcgttttg ccagattgcg ggttcggtgg tgggcagccg
     1381 aatggctgta ttgaaaggta atcggtttat ccgatacgta tttctcgtcg tcgtcggcgg
     1441 attgatactt cgtttcggtt atgatgtttg gaaaatgatg gcgtaaaaaa ttatgccgct
     1501 ttttgccgcg ctataagcag cgattcaaaa actttgagcc cgtcattttg acctaaagcc
     1561 ggatctgcac aacgctccgg atgcggcatc ataccggcga cattaccgtt ttcaagaatt
     1621 ttcttgacaa ttcaaggttg ctatagtatc attacacgtc gtatgaaatg ggatgagggg
     1681 atttctaagt agaatccaat ccaatccaat ccaatccaat ccaatccaat ccaatggttt
     1741 tttttaatca ttattggagt acttaaaatt gttatttgaa ggaggtgaac gacttcatca
     1801 aataaaggca gatgccgtaa ttaacttgct ttgtttaacc tggaacaaaa aaaggaagaa
     1861 caataaaagg aagaacaata ttaccgaatt cagtgcttcg gagctttgaa ttttttagta
     1921 ataccgcctt cctcagttgt aactatagga tgttttacgc ctaaatgcaa cctgttcctc
     1981 aaccatggag ttcccatgaa aaaatttcct ttgcttatgg tcttcgtgat gattgcttcc
     2041 tgtaccgatc atcaacagat ggacaacgca tcgctggaag atgtggaaat tcgcgaaaca
     2101 cttaatgaga tggcccagac tcttgcaagc gatcccagtt ggatcgatca aactcttatt
     2161 gaaaaattaa aaaaaggcgc tgtgaaatcc gccgatctgc cgagctcaat aaaattgtcg
     2221 aagagtaacg gatttgttca ggatattgaa atcgagatgt atgacaattc caatgtcaag
     2281 tccctttcta aatctctagg tacaacacct tactatgtag gccttgatcc ggacgtgtat
     2341 aatttctctc cttccattca ggaagaattg actggtcttc ctgtgatgga tatgcccgtt
     2401 ttctattttg acggacagtc cgtatcggaa acgaccgttc aactcgatcc cgataaacaa
     2461 accgacaaac tcattttttt tattaaatct acttccttgg aaccccatac tctttccaaa
     2521 agcatgcaga ttgccggtac tttttttgca tgccgatcga ttaaagtgaa agtaaaacaa
     2581 gacgaaagcg atgaagaatt tgaagtgtat ttttcaaacg gatcacatcc aatcaatagc
     2641 tactttaacg gtacgacaga tcacgttttc aatggcaata ccagagagga tgccgcagga
     2701 aattcacgtt attatatgga tgtaaacgac aagaacttat ggtatccgac gtctgatggg
     2761 caggaaatct cgttgatccg cgttgataat ctgacagaca gtgttcgtat ggtagctatc
     2821 gaggcggatt ggagttcggg aaattatgtt cagggtcgat acaatcgtaa tcagcaggtt
     2881 ggtaccacga gcgagattct caccgactac tatgacatga cggacaatac caaaaagctt
     2941 caagagaaca gcgcctatta tgttgatttg aagacatcgc tcaatgatga tagatatcag
     3001 aaaggcgcca tcaagagcat aaatgcgaga acactatacg ctcgtacaaa caacggtgaa
     3061 cactatattg aaactgcaaa cgctgatggg ggcgctttga cagatgtaga ttggattatt
     3121 aagcagattg tacagtaatt aatctttttt cttttcagac tatccggttt tgcgaccgga
     3181 tagtttgtta ttttaggagc cctatgaata aatttgtttt agcttttata tccatcgtac
     3241 tatgcggatg ttccggttgt acggatgata aaaaaaagcc atatgaaggc ttcttggata
     3301 aagatcgtga gtttgtcatc gcacatgtag ccccggacga ggatcggatc aatcctgtcg
     3361 aagttttcgt aggacgactg ttgaccattg ttcccgactc aaccgacggt gccaatattc
     3421 aagattacct tttgaaaatt cagttacgtc agtttcaaaa cgaatactat gcgctgctga
     3481 agtatcacga cgacgcacag gtatttataa cgtcttcggg tcagacggtc gagcttcaaa
     3541 acgtgggtaa ggggacctat cgtgacgtat ccaatgcgct acacatctcg gcgcttcaaa
     3601 cctgcacgct caaggtcact aggccggggg gtaaggttta tcaagcgatg gtcagagttc
     3661 cggatcacat tacgatcacg agtttgtctc ccggagatac gaccgtgctc tatcctaaga
     3721 agaatacgcc cgagagtgat ttctgcggtg cggtcgctta tatatcaggg cccgcggtga
     3781 ccggcgccta tatttacagg tctaagtttg atgagaccgc gcacctacag tcttctaaca
     3841 atagtggttc tgcggtaatc atcgaattat gcggaggtgt ggagtacaca aatatatccg
     3901 agaccgtcat ggcctttgat acggtactga ctagagcatg gggcatgggt gaagatactg
     3961 ggttcgggta tgatttgttc gactcacttt tgcattggcg atcggcggcc actccgatta
     4021 cgtaccgttc gaatattaca aatcaggacg gagctaaaat cgcgggctac ttcggcgcct
     4081 ataattccat caacacgcat tttgttgtga aatcgctaag ggattcatgc cagtgtaata
     4141 attaaatctg cttatcggat ttatgcagaa attatttcgg gcttgattca gaaaattaaa
     4201 taagtgatgt aaaaacatgg tcatgaaaaa agtttaataa aacatttgtg cgtgaaaatg
     4261 aataacttga taagcatgta aataaagttg tcgagaatca aaacgataaa atcttgttag
     4321 aaaagtttaa agaatttagt tcgcgataga ggaaatcgta aggtttaatt ttatgggtta
     4381 cattatataa attgcatttt gcgatcatat cgttccggaa ttcgcttcac ttcatttcga
     4441 caatgatctg tacaaagtgg tgggcagccg aatggctgta ttgaaaggca atcggtttat
     4501 ccgatacgta tttctcgtcg tcgtcggcgg attgatactt cgtttcggtt atgatgtttg
     4561 gaaaatgatg gcgtaaaaaa ttatgccgct ttttgccgcg ctataagcag cgattcaaaa
     4621 actttgagcc cgtcattttg acctaaagcc ggatctgcac aacgctccgg atgcggcatc
     4681 ataccggcga cattaccgtt ttcattcatg ataccggcaa tattattcag agaaccattg
     4741 ggattgccat cggcgttgat cgttccgttt tccgtgcaat agcgaaaaag aacacggttg
     4801 tcgccttcaa gccgtttgag cgtctcgttg tcggcaaaat aattgccttc accgtgcgct
     4861 atcggcacca tgagcacttc gccttttttc agcgtattgg taaatcgcga attatcattt
     4921 tccgcgcgaa ggtaaatatt tttacacacg tagtgcaggt ggctgttgcg caataaggcc
     4981 ccgggcagta aaccggcttc ggtcagaatc tgaaaaccat tgcagacacc gaaaacggta
     5041 ccgccatatt ttgcaaaatc catcacatcc tgcatgatcg gcgcgaaacg ggcaatggcg
     5101 ccggttcgca ggtagtcgcc atacgaaaaa ccgccgggta cgatgatgag atcaaacgat
     5161 cgaaggtctt tacggtcttt gtgccaaatg tattctgctt tgtggccgat cagttcaccg
     5221 gccgtgtaaa aagcatcgtg atcgcaattg gaacccggaa atacgacaac acccgttttc
     5281 atgataactc cttttttgaa tgcggggaat atagaacatc gccaaacatt gtgcaattgc
     5341 aatttgacat tcgacggatg atcgcttatt ttgcgcgact acaaaaacgg agctaacgat
     5401 tgatatcccg gcatcctgcg ttaacgccct ctgaaaaaaa atccatttgg gggtggtgta
     5461 tgtacgattg ggccaattcg gccttcatac ttaccgttgt cacggcggta ttgccggtgt
     5521 attttgccga tgtcgtcgta cccaaagagg gttttgatat cggcggcaca ctgtatagtg
     5581 cgacatcgct gtggggcttt atgatcagca gctcggcgct atttgtattt ctttgtgcac
     5641 cgatgttagg tgcgatcgct gattttaccg ctgccaaaaa gaaatttctg atggtgtttt
     5701 gttataccgg tagtcttttt accgtacttc tgtttttttg cgggtccggt gatgtatgga
     5761 tgactatgat ttttctgatc atcgcccaaa tcggttttgt cggcggaaac attttttatg
     5821 acgctttttt gccgcatgtc gctccgcccg ggcagatgga ttgggtatcc gggaaaggtt
     5881 tcgcgtatgg ttatatcgga agcgacatcc agtttttgtt atgcctcggt cttattttag
     5941 gacacgatgc gttgggattg accggtacga tggcagttcg tttggtactg gcattttccg
     6001 gtttgtggtg ggccgggttt tctattttta cttggaaata tgtacacgaa gcgcctgcga
     6061 cggaaaacat acctgatgat ttaagaagtt cgcccaaagt catagcgtat attcgtctcg
     6121 gattacgtcg gacggtcggt gtacttaaaa aaatagtttc cacgccgtat ctgttaattt
     6181 ttcttatcgc ttttatgttg tataacgaag gtattcaaac tacgatagtc atggcaacta
     6241 tctacgggaa agaagaactc ggtctctcga cttcggtact catgatcaca ctttttgtga
     6301 tccagatcgt ggccatgttc ggtgcactac ttttcagtaa actcgccgaa cgttggagta
     6361 cacaaaaggc gattcttacg acattggtcg ggtggtcgtt ggtcgtcttg tatgcgtatg
     6421 ggatgactac ttcggctgaa tatatggcgc tgggcgtggt ggtcggcatc gtcatgggtg
     6481 ggtcgcaagc gctgagccgt tcgtattacg gtgcgatgat tcccaaagaa tcttctgccg
     6541 agtattatgc gttttattct gtatttacca agttttctgc gatatggggt ccgcttgtgt
     6601 ttgccgtcat tcggcaagcg accggttcgt cccgtttatc cattgtatcg ctaatagttt
     6661 tttttattct gggtatgata ttgctgacga tggtacgagt tcccaagtca gccgaagtgc
     6721 ctgcatcata ggagttgtat gaagcgttct ccgcttacgg ttatttttat tacggtattt
     6781 attgatctgg taagttttgg tattgtaata ccgttattac cgttttatgc gcagcagttt
     6841 gacgcatcgg gcacgacgat cggtttgtta ttgagtgttt tttcactcat gtcattttta
     6901 tttatgcctt tatggggacg tatttcggat cgttacggga gaaggccggt tttattggtg
     6961 acgatatttt gttcagttat cggatatatc atttttgcca atgcgttcgc actgtggttg
     7021 ctttttttgg cacgtatcat gtccgggatc ggtaacgcga atatttccgt ggcacaagct
     7081 tatatcagcg atgtcacttc ggccaaggaa cgcgccaaag gtatgggatt gatcggcgcg
     7141 gcatttggtc ttggatttgt ctttgggccg ttaatcggcg gtgttttttc cggcgaagcg
     7201 tttggagcgt atcgttttgc gattcccggt tggatcgcag tggggttgtc gttgatcaat
     7261 tttttcatgg cttattttat gctgccggaa tctctggcac aaaccaagca ggatgaaata
     7321 aaagcgcatc atcttataga tgtgcgcggt ttacagcgcg ccataaaaaa agaaaacctg
     7381 ggcttactga tgattttctt ttttattgtg acgattgctt tttccagtat ctatgcgacg
     7441 ttccctctgt ttattatgga agagccattt cgtctgacga gttcggcgat gggatggttt
     7501 tttgtagaaa tcggaatcgt gagtgtaatc attcaaggcg gtttgatcgg cagactgaat
     7561 caccgctttg gtgaacagcg tttgatcagt tttggagcta tcgctatggc gatcggtttt
     7621 attacttttc ctttgtcggc atatttgcca atcggtggta ttgttttact tataatttcg
     7681 accgcgttga tctcgatggg cagttcgtgt ttagcgccga cgacgatggg tcttacatcg
     7741 cgtttggccg atgtgtcaga gcaaggctcg attcttggta ttgcacagag ttttgccagt
     7801 ttaggacgga tgatcggtcc gttcatcggc ggtgcggcgt atgactatgc cggacataaa
     7861 tcaccgtatt attttgcttt tgtgctgatg gccgttgccg cggtcatggc ttttattctc
     7921 aaaagccgtg atctgaaatt ggacgaggcg ccggttgcga tggaaccggt ggccgaagta
     7981 tagttttttt cgcaccatct tgcatttcca tcgttttttg ataaattgaa tccgcttttt
     8041 gtataacgat tgagagaaat tcataattat ggcagtatca tccggaaaaa agaagactac
     8101 aaaaaccgtt aagcctaaag ccgtcaaatc ggataccgcg gaaaagccgg ttaaggaaaa
     8161 atcggttaaa aaaaccaaaa cccccgtcgc ggaggaaaaa acagccgtcg aatccgcacc
     8221 cgccaaacgt acacgcggcg gtcgtaaacc gtttgttcgt gctaagacgg tcaaagaaga
     8281 cattgagatc acaatacccg ataatattga gcgtaccaat aaatcgctgg tgatcgtcga
     8341 gtcgccttcc aaagccaaaa cgatcaataa atacttaggc aaatcatttc atgtcgaagc
     8401 ttcggtcggg cacatcaaaa atcttccgtc gaataaactc ggtattgata tcgaaaacgg
     8461 ttttaccccc gagtaccgca ttattgacgg caagcaggat gtcatccgta aactaaaaac
     8521 gttggccgct tccacaaaaa acgtatttat cgcgaccgac cctgatcgcg aaggtgaagc
     8581 gatcgcatgg cacatcgccg gtgaaatcga aaagcataat cccagcatat cgcgtgtact
     8641 ttttaatgaa atcaccaaaa gcggtgtcga agaggcaatg cgcacaccga cgaaaattga
     8701 tcccaaatta gttcagtcgc agcaggcgcg ccgtgtgctg gatcgcatcg tcggttatgg
     8761 tgtgagtcgg tacctgtggg gtgttgttcg ccgcgggaca tcggcaggtc gcgtacagtc
     8821 cgttgctttg caactggttt gcaaacgtga agaagagatc aatgcttttg ttcctcagga
     8881 atattggagc atccatgcca aagtgcagga tgaaccgaca gaggcctttt tggcacatct
     8941 gcattccata gacgggaaaa aagtagatat agccagcggc gacgttgcac accgttttgt
     9001 cgaagacatt cgtaagaaaa attttctgat caaagatatt cgcaagcgtg aagcgcgacg
     9061 taatccctcg gcgccttttg ttacgagctc gctacaacag gaagcttcac gaaagctcgg
     9121 tttttcacct aagctgacca tgatgctcgc gcagcagctg tatgaaggta ttgaccttgg
     9181 tagcgaggga cttacgggtc ttattaccta catgcgtacg gattctacgc gtgtggctga
     9241 tacagcgctg gtgtcggtgc gtgaatatat catgggcagc tatggccgcg aatatattcc
     9301 tcatacgcct aatcagttca aaagtaaaaa agccaatgtg caagatgcac acgaagcgat
     9361 acgtcctaca aatgtgaaat tcacgcctaa aaccgtgaat aaatatctca cgccgcaaca
     9421 ggccaaactg tatgaattga tttggaatcg ttttgtggct tcgcagatga ctccggctga
     9481 gctggatcag acgaccattg atatttccgc cgacgaatac ctttttcgta ctacaggttc
     9541 cgttgtgacg tttaaagggt ttttgcaggt atatgaagaa ggccgcgacg aaggcgataa
     9601 gacgcaggac aacggtgaag aagaggataa cagcggttta ctgccggcca atctgaagat
     9661 caatgatgta ctcaagttgc atgaactttt gccggagcag cattttacca aaccgcctgc
     9721 acgttttacg gaaagttcgc tcataaaaga attagacaat atcggcattg gccgtcccag
     9781 tacttacgcg atgatcgtcg gtacgatcgt ggaacgcgaa tacgccgaac tgcgcgagcg
     9841 caagctgtat gcgaccgatc tcggtaagag cgtcaataaa gtgctgaacg atcattttaa
     9901 tgcgcttttt aacgtacagt ttaccgcgga gatggaagaa gagctggata agatcgaatc
     9961 cggagaactg ggttataaaa aagtactcga tgatttctac aatcctttcg tgaaaaacat
    10021 gaaggatgtg gaaagtaaaa aagatgcgat caaagaatcg gtgcaggaaa aaacggatga
    10081 gacctgcgaa atctgcggta aaccgatggt gatcaaatgg gggcgcttcg gaaaatttat
    10141 gtcttgtacg ggttatccgg aatgtaagaa tatcaaaaaa gtatcaaaag acggcgaagc
    10201 cgcgccaccg ccggaagtga ccgatgaaaa atgtccgaac tgcggaagcc ctatggttgt
    10261 acgtcaaagc cgcggtggca agtttctcgg atgttccaat tacccgactt gtaaaacggc
    10321 taaagcgcat ccggatgata ttatcaaaga cgtgatttgt cctaaagacg gcggtcagtt
    10381 gatacgccga cgttcacggt atggtaagtt tttctacggt tgtgcgaatt atcccaattg
    10441 tgattttgta tcgtggaatg agccggtagc gacacgttgt ccgcattgcg atgccgcgtt
    10501 tatggtcaaa aagaccagca aacgtaaagg tgatttcctt ttatgtatga cttgcaatca
    10561 cgaagagatt ttgccggcgg atacgtcggt gatgaaagaa caacctgaaa acgaatcggt
    10621 ttcctttaat gaaggggaat aaattttaag gttcagttta atatttagga ggagaaatat
    10681 ttatggcgga tgaaaaggaa caaaaagacg aactattgga aaaaggcgcg gcgctgagta
    10741 cggcgattat cggcgtattt ttatccattt cttccatttt gggcggcaat gccaatgatg
    10801 atatcatgat tcatcgcggt ttggccaata atcaatggtc gtatttccaa gcgaagagca
    10861 ccaaacaagc gctctatgaa gtaaatcgta aaatgtttga aacgcaactt gctgatccgt
    10921 ccctggccga aagctataaa aaagaaattc aggcaaaact tgagaagttt gattcggaag
    10981 tcgcgcgtta tgataccgaa aaaaaagaga taaaagccaa agcggaagaa catgaaaaac
    11041 tttccgatca ggccgctgag atcgaagata aatacgacta tgccgaaggt ttctatcaaa
    11101 tagccatcat tcttgcggct gtggcaatga ttgcaggcaa tcgcaaactt tggttcttca
    11161 gtatagtact gggttctatc gctatcgcaa taaccggatt tgcttttttc gtttctcttt
    11221 agtataagtt agtattacag aatgattgat aataaaaaac gatttaataa atgaagattt
    11281 cagcgctaaa attaacgaac atactttcgt gccgaatgtc taacggtaag cgaaaacgta
    11341 tggttatgca ccatcgaatg cggcgctgaa cgtctgatct agttattttt atatttttaa
    11401 taacagccgg gttcacagcc cggctaattt ttttaggagt aaatgtgaaa aaaataaaag
    11461 taggaatttt gggagcgaca ggcaccgtgg gtcagcgttt catcgagttg cttcacgccc
    11521 atccatattt tgagattact gcattggcgg cttcggaacg atccgtcggg aaaaaataca
    11581 acgatctcaa tacatggaaa ttagacagcg ttttgccttc gcgtatcggg gaaatgatcg
    11641 tgcagccatg ttctccggga atggatgcgc gtgtgatgtt ttcggcattg gattcttcgg
    11701 ttgccggtgc gatcgaaata gagtttgccc gcgccggtta tattgtcgtg agtaatgcca
    11761 aaaattaccg gatggatgaa gatgtacctt tgctttttcc ggaggtcaat gcagatcaca
    11821 cgcggttaat agaaattcag aaaaagaatc gcggttttga tcgagggtat attgtaacca
    11881 attccaactg cgcgatcatg gggtttgttc ctattgtgca tgtattgcat cagcattacg
    11941 gtgtcgtatc ggtagatgtg accacactgc aagcgatttc cggcgccggt tatccgggcg
    12001 tgccttcgat ggatatttta ggaaatgtga ttccgtatat ttccggtgaa gaagaaaaaa
    12061 ttgaaaccga acctttaaaa atttggggac aaatgttaca ggatcgcatt cagttcgctt
    12121 cgattgcgat cagtgcctcc gtacaccggg ttcccgtttt ggacgggcac ttggcgagtg
    12181 taacagcgaa attaaaacag ctgcctgcaa tcggcgatat caaacaaaaa ttatcagaat
    12241 ttaaaagcgt gccgcaggaa ttgaatttac cgcttgcgcc gatgaggcct atcgtcgtca
    12301 ttgagggcga tgaccgtccg caaccgcgcc gtgacgttac tttggaaaaa tcaatggccg
    12361 cgtcggtcgg ccgtattcga tacgaccgtt caggcaacct caagttttct tgtttggttc
    12421 ataatacggt tcgtggtgcg gccgggggag cagtacttaa tgccgaattg cttcacgcta
    12481 aaggtttatt ggaataaaaa aaaacaaata aatgaaaagt tttttaatca aaacgatgat
    12541 tcctcttgac aagggcgggg tgcgacgtat atattatttc attatgattt tctttagcaa
    12601 cacaaaaaca gctcagaata taatgcccac catgatgcag atgtgcatgt gcacctgtac
    12661 gcggaagggc tatatccatt tatagcaaat aagtagaata tttttacttc aagcccttcc
    12721 gataaaacgg aagggctttt tgttttacac tcaaacaaag aataaaatga cttaaacaac
    12781 taacgacata cgctcttatc gcgaaaggct gagggatcag gccctgtgaa gccttggcaa
    12841 ccgatgcata gtgacgtcct gtgcatgccc ggtgccgcat cctgcccgcc gatactgaag
    12901 ggtattggtt tcgggaaaga tgagagaaga agcctaggcc tcttccaatc tcccgggaga
    12961 ggtttatttt ttttaaaatg aaaggaacat catgaaattc ggaacttctg cgatccatgc
    13021 cggggtcaac aaagatacgg cctacaacag cgtcaccaca ccgatctata cgtcttcgac
    13081 atttcgattt gaagagccgg gacttactaa aggatatgat tatacgcgtt cgggcaatcc
    13141 gacgcgctcg gctttggaag aaaatatagc cgcgcttgaa ggcgggatat gcgcgcgtgc
    13201 cgtagctaca ggaatggcgg ccgtcgcgac ggcgctgcat tttttcaaaa gcggcgatca
    13261 tattttgtgt acacatgatt gttacggcgg cacagaaaga ttactgagga cgtacagtga
    13321 attgtttggt ctgcgtgtta cgtatgtaga catgtgtgat ctgaaatcgg tagaagctgc
    13381 atggttgcct gaaacgcgcg gaatatggat cgaaacgcca agcaatccgt tgctcaatat
    13441 cgtggacatc cgagcgatta cggctatagc gcgtgcacga agaggtttat ccattgtaga
    13501 taacacattt ctctcgccgt atttccagcg gccatttgaa ttgggagccg atatcatcgt
    13561 gcattcgacg actaaatacc tcaacggtca cagcgatgta gtcggcggtg ccgtggttgt
    13621 caatgactca aatttggccg accgtctcaa atttcttgtt aacgcattgg gtttgggtgc
    13681 ggcgcctttt gattgctggc ttgtactgcg cgggatcaaa acccttgtgc cgcgcatgca
    13741 agcgcatgag cgcaatgcgc tggcggtagc gcgatttctc tccgaacatc cccaggtacg
    13801 gcgggttttt taccccggac ttccgtcaca tccaaatcat gccttggctc gatgtcagca
    13861 atacggtttt ggcggaatgg tttcatttga actggacggg gactcggagt ctatgcgccg
    13921 gcttctcaaa ggtaccaaat tgtttacgtt ggcggagtcc ctcggcggcg tagaatccct
    13981 gatcgaacat ccggccacaa tgtctcatgc ttcgatggat gccgagttgc gggaagaagc
    14041 cggtatcaat gaccggattg tacgcttatc ggtcggtatc gaagaaacat ccgatttgat
    14101 tgaagacttg gcgtcggctt tgcgtcagtg tcaatacaat acgtctgcaa cgcccgcgtc
    14161 ggcgcgatct gttttttcat caatcaatgc tcattaattc atacataaac acgtaaagaa
    14221 aggtccatca tggctacgga agaaaaaaaa tcaacctacc gttttgaaac attgcaactt
    14281 catgcgggtc agaaaccgga tagcgctacc aatgcgcgcg ccgttccgat ttatcaaagt
    14341 acgtcgtatg tatttaataa tgccgatcat gcggcagcgc tttttgggct acaggaattc
    14401 ggcaatatat acacgcgcat tatgaatcct acgacggctg tttttgaaga gcgcatagcc
    14461 gccttagaag gcggtgtcgc ggcggtggct acatcgtccg gtcaagcggc gcaatttctg
    14521 gctatcacga cgatcgctca ggccggtgat aatatcgtcg ctaccagttt attatatggc
    14581 ggaacataca atcaatttaa agtcacttta ccgcgtctcg ggattgacgt gaagtttgtc
    14641 aacggggatc gtccggaaga ttttgaaaga ctcattgata cgaaaaccaa agcgctttac
    14701 atcgaaacga tcggcaatcc gcgattgaat attccggatt ttcaggcgat cgcggacgtg
    14761 gcgcaccgtc atggtattcc gctgattgtt gataatacat tcggtgcagg cggctacatc
    14821 gcacaaccca ttcgttttgg cgccgacatc gtggtgcact cggcgaccaa atggatcgga
    14881 ggccacggca cggcgatagg cggcgtgatc gttgatgccg gtacgtttga ttggggtaac
    14941 ggtaaatttc ctctgtttac cgaacccagt ccgggatacc atggactaaa attctgggat
    15001 atttttggca gtgcggggcc gtttggcaat attgcttttg ccatacgtgc gcgtgttgag
    15061 ggtttgcggg atatcggccc cagtcagtca ccttttaatg catttctgct cctgcaaggt
    15121 ttggaaacgc tgtcgttacg cgtcgaacgg catgcccaaa atgcgttagc actggcacat
    15181 tggttgaaaa aacaaccgca agtatcgtgg gtgtcgtatc cgggttttga agatcatccg
    15241 tatcacgcca atgccaaaaa atatttacgt ccgggtcact atggcggagt gctggtattt
    15301 ggaattaaag gcgggaaaga agccgggaaa aaatttatca acagtctctc tttagctagt
    15361 cttttggcca atgtaggcga tgctaaaact ttggtcattc accccaattc gaccacgcat
    15421 cagcaattaa gcgatgccga gcaacacacc tccggagtga cgccggattt gattcgtgtt
    15481 tcagtgggaa tcgaacatat cgatgatatt atcgctgatt ttgaacaagc attgcataag
    15541 acggaatctt tgactgtgga ggaatccgcc gcatgaatct gcttgtatct tcgatgccat
    15601 cggttttttc gacagtaaaa tatacgacgg tgtttgatac gatccccttg cgtcttcagc
    15661 aagggggtac tttatcgaac attactgtgg cgtatgagac ctatggtaca ctcaacaacg
    15721 aaaaatcaaa tgccgtttgg gtctgccacg cattgacggg taatgcgcat gcctacagcg
    15781 acggtacaaa cgtacccggt tggtgggacg gattgatcgg aaaaaatttg gcacttgatc
    15841 cggacgaata cttcattatt tgcagtaata ttctcggcag ttgttacggt acgaccggcc
    15901 cggcatcgca ttatccggcg gacgggttac cctggcgcaa acgatttccc ctcatcacag
    15961 tacgcgatat ggtgcaggta cagcacgcac ttttggaaca tttaggcatt gagcgactgc
    16021 aagccgttat cggcggttcg ctgggtggta tgcaagtgct tgaatgggcg ctgatgtatc
    16081 cggaaaaagt tgcgcggatc atacctattg ccacggcagc aagccattca gcttgggcta
    16141 ttgcctggaa tgaagcagca cgacgtgcca tagtccaaga tcctgcattt caaaatggcg
    16201 ggtataacga acagccggtt aagggactcg gtctggcacg gtggattgct atgatttcgt
    16261 atcgctcgca aaaatcttat caggacaaat ttgcacgtgg tggtcagccg tgcgatcaaa
    16321 ttaatcccga ttttttttat tcggatgaaa cggcacaacc attcgcgatt gagcattact
    16381 tggattacca aggaaaaaaa cttacagcgc gttttgatgc gctcagttac atccgaataa
    16441 cgcaagccat ggaccgtcat gatgtgactg aaggccgggg ctccttggac tatgtactgc
    16501 gtagcatacg tgctaaatct ttgtgcatag gcattcgctc ggacgtgctt tatcctgcgg
    16561 aggaacaaaa agcgatcgcg cggggaatta aaaatgcacg ctatatcgaa ttggattcgg
    16621 atgacggtca cgatgctttt ttgataaaaa atgaattgat gaatgaaacg attgcaaaat
    16681 ttttaaagga aaacgaatga tatcgttaaa agataaagtt gccttgatca ccggaggttc
    16741 gcgtggtatc ggagccgcca cggcgatatt actggccgag gccggagccg atatagccat
    16801 aaattattta aacagtgagg cgaatgcccg gcgcgtagtt gatcgcattc aggcattagg
    16861 tagaaaatca gccgcaatcc gtgcggatgt cagcgatgac gcgcaagcca aagaattggt
    16921 gcaatcggtg attctgaaat tcggacgatt ggatattctt gttaataatg cgggtatctg
    16981 gacgcgcgct ccgatcgggc ttaccgataa atccgtgtgg gatgctacat acctcactaa
    17041 tgtacaaagc tatttatccg tgactaatgc ggccgttccg catttgatga aatcacgcgg
    17101 tcgtattatt aatatcacgt ccacagccgg ccagcgcggg gaagcatttc acagtcatta
    17161 tgccgcatcc aaaggagcga tcactgcgtt tacaaaatcc atagccgtcg aactcgggcc
    17221 gcagggtatt ttagtgaata atgtagcacc gggttgggtg gacaatgatc ttaaccgcga
    17281 agttttttct gatgcgggtt tcaaaaaaac cgtgattgag agtatcccga ttcgtcgtat
    17341 tgcgtcctcg gaagatgtgg ccggtgccgt tttatttttg gcatcagatc tgagccgcca
    17401 tatcaccggt tcgacgatca gcgtcaatgg gggcgccgta ttagtttaaa ttcaattata
    17461 attttatgag gatatcatga tagttatgaa atttggcggt acttctgtgc aggatgcggg
    17521 tgcgattcgc cgggtggccg aaatcgtcag acgccacgct gaacgcaagg tgctggtagt
    17581 ggtttcggca tgtgccggtg tgaccgatca acttgtacgg ctgagtcagg ctattgaaag
    17641 gcgtgatgaa gccgctatcg gcaagataaa agaaacatta attgataaac acaaaatgga
    17701 ggctcaaacg ctaactttgt cggcggagga aaaagagcaa ttagaacgat ttaacggtga
    17761 atttgaacgc tgtgtcaacg gcgcatccat tttgcatgaa gtgacgccga ggacgcatga
    17821 tcgtcttttg tcgttcggtg aattgtggtc atcggttctt actttgtcgg cgattcgtca
    17881 gacaggtcaa agcatttcgt ggttggatgc acgaaccgtg ataacaacgg ataatgccta
    17941 caacgccgcc catccacaga cagatgcgct gccgttacaa gttcagcaaa aaataatacc
    18001 cgccttagaa aggggcatcg tgatcaccca aggttttctc ggtgcaacga gggacggtca
    18061 cacaaccaca ctgggccgcg gtggttcgga ctacagcgca tcacttttag gtgcgtcgct
    18121 caaagcggat gaaatacaaa tatggacgga cgtggacggt attctcacaa cggatccgcg
    18181 cctggtgcat gtcgcccgtc aactgacgca tgtcagcttt gatgaagcta cggagttgtc
    18241 gcaatttggt gctaaagtat tgcatcctaa aacggtatta ccggcgattc aaaagaacat
    18301 tccaattcgt gtgcttaaca cattcaatcc atccaatgcc ggtacatgca ttacgcggga
    18361 ggcgatcaaa ggaaaaggaa ttgtccgttc gattgcgttt aagcggggct tggaagttat
    18421 gacggtacga tttaatgatc ctgaacaaaa gagcccgatt cttgcagaaa tttttgctcg
    18481 gttgcaggaa gcaggaaccg atgttgaatt ggtaaacacg acagagcggg ggattgttct
    18541 ggcggccgct tccaatgaac gttggcgggt attttgtgat cacctgccgg cctcatggca
    18601 tattactttt cagaccggac gggcgttgtg tgcattggtt ggtcatttga tcgccgaaga
    18661 tttttccaac ctgacaagag cattgagtgt gatgcaatcc gaagccattc atgtcgaagc
    18721 gattgcacaa gggggatcag ggcatcactg ggcggtgctg ctccgcgaac atcagcttac
    18781 accggcggtg cgactcttgc atgccgattt ttttgaaaac agagcatccg gccaaaccgt
    18841 agccgttgcc tgacgacgaa acaatattta aataaatacg taacctcata cctaatctaa
    18901 acacatccgt tgttgttatt aaatgatgag gtttgtcatg aaaaatgcgg ggtttttttt
    18961 gtgggtttgg gtattcagta tttcgttgtg cgtgaaagaa ctgaaagccg gtggtgatga
    19021 gtattggttt ctcaaaaatc aggcccacac cacgcctgaa aataaactgt atgatacacc
    19081 cgattggcgt attgctctgg atggtcccat acgcggttcg gcagcttttt cgggcggtca
    19141 cgtatatatc ggcacagcca aaggaacgct ttatgcggtt cgcgaaaaag acggtacggt
    19201 agaatggaat cgtaattttg gctcggctat tcattctacg ccggcggtaa aaggcaatca
    19261 tttgtatttt actgacaatt cacagcattt gtattgtgta tcggcgggtg acggtaaaga
    19321 aaaatggcgt tatgcttttg ataaggcatt gccttacgat tggaagtttg actattggta
    19381 ttcatcgcct gttttttggg gcgaaaatat tatcgtgggc ggtgatgatg gctacctcca
    19441 tagtgtacgg ttacgtgacg gtaaagcaca atggaaatat aaagcggaat ccgtgattcg
    19501 ttcaacaccg gttatcagag acgggattat ttattttggc gattgtatgg ggtatgtcta
    19561 tgcagtagat gcagcgaccg ggaagtcgaa atggaggagt gaaaccgaag gtgtacgcat
    19621 agatcaatca cagtacattt ttgatcgcaa agcggtgatt agttcggctg cgttgtccga
    19681 ggatgtttgt gtcatcggca gccgcgatgg atttttatat ggttttaata cgatagatgg
    19741 taaacaactg tggtctgtgg attataaaat aagttgggtc aattcgaccg cggctattcg
    19801 tgaaggtatt gccgtgacgg caacatccga cggacgttat gtaaatgccg tggaagccaa
    19861 aaccggtaaa gaaatctggc gatttaatac gcgtcaaatt ttatggtcgt cacctctgat
    19921 catcaacaat catgtgtatg cccaaagttg tgacggtgtc atgcatattt taaattttaa
    19981 aaccggtgag aaaatttcag aacatcgtac gatcacgccg atccaatcgt tttcgtcacc
    20041 cgtatttaca ggcaaaacgc tgctttatac caatgacagc ggtattctgt atgcgtcgtt
    20101 tggcagaccg acccctgtcg aatcgcgtac acggaaactt gcgctttgga tggataaagt
    20161 aggactgcag tttggtaaca atatggatgt aagattgaaa aattatctta acgatattgg
    20221 ctacgaggcc attgatacca atcgttttgc tgaaatctta acacaatctg attcggcccc
    20281 aaaaacaata gtggtagtga tttctgcggc atttccggat acgatattgc aaggcgcaaa
    20341 aaacagcttg gttcgccgat ttcttgatca aggcggtgcg atcatcaatt ttggatttaa
    20401 tcctaccgtg ttcgatccgc gcattgagac ctataatttc caaaaagcgg aagaaatttt
    20461 cggagtggct tatggtccca atgatctccg cagttttcag ggtttatatc cgtcgtttgc
    20521 taccgaactt ggaaaaaaat atcagttgcc tgatttttgg acgggttttg ccggagtgaa
    20581 taaaaatcag gtggatgaag tgttaggact taatgaaaac gggttagctt catcctggat
    20641 taaatattac ggtgccaata aaaaaggagc gtggatacaa atcgccatgg atcgcagcaa
    20701 tccggttgat tataattttt taaagtattt taacgctaaa tagatctaag caactacatc
    20761 gagttctttt aaactcctaa catgtcgtat gttaaaaata cgatattttg ggagtttttt
    20821 tatggactgt acaaatgaaa gccagccttg cattaataag gaattttgat aaattacgag
    20881 gatttaacat atatcaaaat ttcttggata ttttgtgccc aaacgtaccg acatcaaatc
    20941 aattttagtc attggcagcg ggccgatcgt cataggtcag tcttgcgaat ttgactattc
    21001 cggaactcaa tcctgccgtg ccctcaaaga agaaggctac cgtgtaattc tgatcaacag
    21061 taatcccgcc acgatcatga ccgatccgga aacggcggat cgtatttata tcgaaccgat
    21121 tacgccggcg atggttgaaa aaataattga gcgcgagcgc ccggatgcct tgttaccgac
    21181 tatgggcggc caaacggcac tcaatacgtc ggtggagctt ttcaaatccg gcgtgcttga
    21241 aaaatataat gtcgaaatga tcggcgcaaa gtatgattcc attcaaacag ccgaagatcg
    21301 cgaacttttt aaagaagcca tggaacgcat cggcttgcac gtacccaaag gacgatttgt
    21361 tcgtacagtg caggacgcca acgaagcggt atccgaattt gattacccga tcattttaag
    21421 accgtctttt acgctgggcg gaacgggcgg cgctgtagca tataacattg acgaatataa
    21481 cgatctgatt gtcaaagctt tggcagcgag tccgatcagt gaagtgctcg tcgaagaatc
    21541 cgtgatcggg tggaaagaat atgaactgga agtaatgcgc gataaaaacg ataacgtcgt
    21601 cattgtctgt tcgatcgaaa atttcgatcc gatgggcgtg cacacaggcg actcgatcac
    21661 cgtcgcaccg gcgcaaacac tgacggataa agaatatcag aaaatgcgcg atgccgcatt
    21721 gaagattatt cgtaaaatcg gtgttgaaac cggaggttcc aatattcagt ttgccattaa
    21781 tcccgctaac ggcgacatga tcgtgatcga gatgaatccg cgtgtgtcgc gcagttcggc
    21841 gttagcctcc aaagcaacag gatttccgat tgcaaaaatc gccgctaaac ttgctatcgg
    21901 atacacacta gatgagatac cgaacgatat tacgaaaaaa accccggcat cgtttgaacc
    21961 gacgattgat tatgtagtta ccaaaattcc gcgttgggat ttcgaaaaat ttcaaggcgc
    22021 caatacaacg ctcaatgtgc agatgaaatc ggtcggtgag gcgatggcca ttggccgtaa
    22081 ctttaaagag tcattacaaa aagctttgcg ttcgctcgaa aacgggcgat ttggacttgg
    22141 cgcagatggt aaagacaatc cgccggcaga tgcgtcagat gaaacggccg tcgaagcgta
    22201 tcgtcaggaa gttatcaata aactccgcgt acccaattca caacggatgt tcgccatacg
    22261 tgacggtttc aagatgggta tgacggtaga agatattcac cgctacacat ttatagaccc
    22321 ctggtttctc aataatatcc gtgaaataat cgaattggaa ggatcgctcc gcgcggcgtt
    22381 ggcttgattg gtaaacaata caaggcttga tatccgttaa gcgtttcgct cccctctgtt
    22441 atttcctatc gaatcaataa ttccatgaaa gcacttgcgc tcatagcttg gctgtttgtt
    22501 ctatcctttc ccgtttttgg tcaaatcgaa atttcattta atcacgtcac aactaaacat
    22561 ggtctttccg agggatcggt gcatactata ttacaagacc ggcgcggttt tttgtggttc
    22621 ggtacgcaag acggtcttaa tcgttatgat ggatatacgt ttgctatata caggcaaggt
    22681 tctccgcgtc aagcgctaag cgataatttt atatatacgc tgtgcgaaga tacggcaaac
    22741 ggcatatgga tcggtacttt caatcaaggg ctttgccgct ttgatcgtat gcgcgaatca
    22801 tttacgattt acaaacatga ttctacggat gccacttcgc tcggagataa taccatacgt
    22861 gcgctgtatc gtgatcgcaa aggaattttg tggatcggca catttcgcgg cggcttggat
    22921 caaatgacgg cggatgggaa attcaaacat taccgtcaca atccccatga tccggccggt
    22981 ttgtcgtctg acaacgtgtg ggcattgtat gaagatagcc gcggtaattt ttgggtaggg
    23041 acggatggcg gtggtcttaa tttgttggat ccgatgacgg gaaaatgtac cgttttcaaa
    23101 aatgaaccgg gtaatattcg atcccttagt cacaacacgg tgcgtgcgat ttacgaagat
    23161 cgtgccggtg tcttatgggt ggctacatcc ggaggggggc tcaatcgttt tgattatgtt
    23221 acgcgtactt ttacaacatt caaaaatcat cggaacgatc ctcagagttt gagtcatgat
    23281 tttgtacggg ctttgtatga agacaaacgt ggaaattttt gggtaggcac gcttaacgga
    23341 gggttaaatt taatggatcg tgttaccggg aagtttatac gatttcttcc tgatgttacc
    23401 gatccgggta gtataattca taaaagcgta atatctatag tggaagatcg atccggcacg
    23461 gtgtggtttg gtacatgggg tggcggtgta agctatatca gtcccacggg acatcgattt
    23521 atcaactatc gttatgatcc gttcaattcg tactctctga gtcaagatcg catttatgcg
    23581 ctgcatgaag atcagtcccg cactatttgg gtcggaacat tcggcggtgg tctgaaccgg
    23641 tatttgccgg atcagaaaaa atttcttcat tttcaaaaac cccaacacgg tctcagtcat
    23701 gatgatatat ggagtatagc cgaagaccaa aacggtgatc tttgggtcgg taccaatgaa
    23761 ggagggctca atcgttggga ccgaaaaagg ggaacttttg aagtttttcg caatgatccg
    23821 catgatgcga attcgcttag caacgataat gtttttacag tttatgtgga tccgttaaat
    23881 accgtttgga ttggaacctc cggcggcggt cttaatcgtt ttgatcgtaa tgcgcgtacg
    23941 tttcgtcgtt ttacgcatga tccggcgctt ggatatacgg tcagtagcaa tcaaattcgc
    24001 gcgttgcacc aggataaaga aggattgttg tgggtcggca cgttcggcgg gggcatcagt
    24061 gtgtgggatc gaaaaaaaga aatatttacc cggcatttaa gttcaaatta cgattcgtcg
    24121 tcgctctcaa gcgatcttgt tttacgtatt tttgaaagtc gtgacggtct gatatggatc
    24181 gggacagccg gcggcggtct taatcgttac gataaacaga cgggtcattg gaaaacatat
    24241 accgataaac atggtttagc gaacaacgtc attaacggta tcttggaaga ccaaaccggc
    24301 cgcctttgga tcagtaccaa taaaggcctg agccgattag accctaaaac ggaaacattt
    24361 cgcaattttg atgagggcga cggcttgatc tctgcggaat tcggtgtcgg tgcggcctta
    24421 cgcgcccatg acggccgtat gtattttgga agtaacagag ggctggtgac ttttcatccg
    24481 gacagtatca aagaaaaccg ttatgtacct ccggtaataa tcgcgggttt caaaaaattt
    24541 aacaaaccct tcgtcctcga acgatccgtt tctgaaacgg acacgatcat tatttcatac
    24601 aaagataatt tcatcgcgtt cgaatttgcg gcactcaact atacccatag cgaaaaaaat
    24661 ttataccgct acaaattaga aggttttgat cgcgactgga tagacgccgg tagccgccgc
    24721 gaagcgacgt ataccaatct ggacggcggc acgtacgtat tcagggtcaa agccagtaat
    24781 aatgataatg tgtggaatga ggaagggcgt gcgatcacct tgattgtaga accgccgttt
    24841 tgggaaacag tgtggtttca attcctggta ggtgcctttt tatgcgcggt tcttgtgtat
    24901 cgcttcaggc aaatacagaa tcaaaaaaga gaattagaac gtcaagtcca ggtgcggaca
    24961 cacgaattag agaaagcgat ggattcgctt aaacgcgctc aggttcagat tcttcaatcc
    25021 gaaaaaatcg gttcattggc acatatggtg gccggcattg cgcacgagat caataatcct
    25081 ctgacgtttg tgtattccaa tattcaactt cttcgcgcgc gggttcaacg attatttctt
    25141 ttcatggggt ttcttgataa acgctcacac acggaatcat ggacagatga tgaaaaaaaa
    25201 cagtatgaaa aattcaaaca taccttggat tacgactacc tcgaaaaaga cctggatgac
    25261 atgtttcgaa gttctgtaga aggtagtacc cgtattatca aaatcgtaaa tagcctgcga
    25321 gcgtttagca gtctggatca ggaagaaata caagaaacca atttgctgga gtcgctcaat
    25381 atggtggtgg acctgtttat gcgccagcat aaagatattg ttattgaacg caaaaccacc
    25441 gaaattccct ttataaccgt tcacttgacg cttatgcatc aggctttcat taatatcctc
    25501 gataattcgg tcaaagcaat ccgcgatgcg gaaaaacaaa atttgctggc aagcggagat
    25561 ggtcgtatta cattatctgc cgaatggcaa tcggataaga ataaaattcg cctttgcttc
    25621 attgataacg gtgtgggcat tgattcatct gttcgaaata aaatttttga tccgtttttt
    25681 acaacgcgac ccatcggggc cggcgcagga ctgggtttgt cggaagtttt tacgacggtg
    25741 cgaaaacatg gcggcgatgt cactgtcgaa agtgagcccg gtatcggtac gaccctgtgc
    25801 attgatctcc cggtaaaaac attatttcac gaaaactacc aacacggttc gtgatgaaat
    25861 ccacaataat gtatagcctg ctctgtttga tttatttttc gccgcttgcg gcgcaagatg
    25921 agcaaggcga accgttcatc cgtaactact tcccccaaga gtatcgtgca caatcacaaa
    25981 actggtctat tgtacaggat catcggggac taatgtattt cggaaacaat gacggcgtat
    26041 tggagttcga cggaatccgc tggcgtctga tccctgctcc taatcgaacg tcgatccgtg
    26101 ctttggccgt agatgaggtg gggacgattt atgtcggagc ggtcggcgag tttggaatac
    26161 tcaaaacaga ttcgacgggt attcgtcaat attattcgct gagcgcatca ctggattcgt
    26221 cgttcaaagg aattaccgat atctgggaaa ttagctgtat tgctgataaa atttattttc
    26281 gtagcaataa atattttttc gtttttcgac gcagcgccga cggttcctat cactttgtca
    26341 aagctattat atctgatact cagtttcacc gttcctatct tatccataat acgatttttg
    26401 tgagacagga caagcgcggc ttatacataa tagatcagga tacgctgcat ctattgccgt
    26461 atggtaaaga gtttgcgaat ttgcgtattt atggtatgct gccgtttaaa gacggacggt
    26521 ttctgatcgc aacgcgcgag aaaaatcttt ttgttgcaac acgcagcggt tttgctgcaa
    26581 tgaaaacttc ggttgatgat tggcttattc aacatcagtt gtattatgca tcaaaactga
    26641 aaaacggtaa tatggttttg gggacaattt tgggcggtgt tgttgaatta tccgaagatg
    26701 gtagttttat tcgcagagta gataaaagtt ccggattatt ggatgacaat atttgggcga
    26761 cgtacgttga tcacaacggc atattatggc tggccatgaa taacggcatt gcgcgggttg
    26821 aaatcggcag tccgtattcg cgatttagtg aattacacgg attgcgcggg actgtgcttt
    26881 cattgacgcg ttggaataat acgctttatg tgggcaccag ccggggggtg taccgtttga
    26941 atcgttatcc ctcatccccg gaaaaggatg ttccgctttc atccagtcag gaaatatgga
    27001 aatttgagcc gatcgccggt atcacggcgc agtgctggtc ttttgtggat aatgaggttt
    27061 cattatttgc gggtacggca caaggtgttt tcaggattaa cggtcagcag gcacaaatgg
    27121 tggataatcg ttcggtgcga aatatgatgc gatccaaacg aaacccaaat caggtgtggg
    27181 ttggtttgtc cgacggatta gcgttgtgcc ggattgataa agaacaatgg gtgcgtgtcg
    27241 aggaacatcg tacgatcaaa gaggaagttc gaacgatcgc cgagtctttg aatggtgatt
    27301 tgtggctcgg tacgcgtttc aaaggcgtca ttcacctcaa acgcgaaggg gattcactca
    27361 actggccggt tacccgttat gaccgagcta acggtttacc catcggcgaa atgtatatgt
    27421 atgaagtgga cgggagaccc ttggtcggga cgacatcagg gctttttcgt tacgatgtgg
    27481 atcataatca ctttgtgcga gaaactcttt ttggtccgtt actttcggac agcgcaacag
    27541 tagttcatca ctttttgcag catccgcagt tcggtttgtg gatgaccgct caagtcggag
    27601 aacgatttga aattttgcat gctgcctcgt catcgcgcgc gatttcttat accgtaagaa
    27661 acccgtttct gcgaatatcg gactttcgcg tcaatatacc gtatccggaa tcgggcaaca
    27721 tcatctggtt tggcggcgcg gacggactta tccgttatga cggcaatatc caatatgctt
    27781 gggattcgca gttttatgca ctgatacgca aagccgtggt gatgaatgat cagagacaga
    27841 atattccgat tttatcggat acatcacagc atgttatgtt ggcgcactat gacaataata
    27901 tccgatttga attttcggct agcagctacg aacgcgacgg atccaatcaa tttcaatact
    27961 atctcgaagg ttttgacccg ggttggtcgg agtggtcaac ggagtttcgt aaagattata
    28021 ccaatttgcc ggacggagtt tttcgattta gggtgcgtgc acgcaatctc tacgaacaag
    28081 tcagtgtcga agatcaattg cggttcgtca tacgatcgcc gtggttcaaa tcatggtggg
    28141 cgtttggctt ttatacggcc ttagttttct tatgtattta tgtgataatt cattatcgtg
    28201 agcggcgatt ggtacttgcc aatgacaatc tcgaacgcat cattcagcag cggaccgcgg
    28261 aattgaaaca aaaaaatgtg gaattagaaa atctgaacga aaaaaagaac gaatatttgg
    28321 gtattgtagc acacgatttg cgtagtcctt tgacaacgat tatgggatat attgatcttt
    28381 tgatcgccga tgcggaaacg gatacattca aaccgaaaga agccgtaaaa gatctagtcc
    28441 gtatttcccg catatcgcat cagatgtcgc atttcatacg agaattgctg gacatctcgg
    28501 ctattgagtc gggcaaaatc cggttagaag ttttgccgga caatctgggt actattattc
    28561 gcgatgcgga accgttgcat cggcgtgccg cgaatcaaaa aaatatcgcc atggattacg
    28621 aatatgatcc gactttaccg gatacattga tggatcgtga aaaaataaaa tcggttgtgg
    28681 ataatcttat caataatgcg atcaaatata cgtatcccgg cggtaaagtg cgtgtgtatc
    28741 atgaagtgcg tgatcaggat gtggtaacgc acgttgaaga taccggtcaa ggccttagcg
    28801 acgaagataa acaaaccgta tttatgagct ttaaacgcct gagcgccaaa cccacggccg
    28861 gagaatccag tacaggcttg gggctggcta tcgtaaagaa aattatcgaa attcataatg
    28921 gtcagacctt ggttcgcagc aacaaagggc aaggttcgac cttcagtttt agtttgccgc
    28981 gggcggacaa acaatcggat aaaaatctaa aggttaaggt ttaacctata tgcagtacca
    29041 aaagtttttt cgaagcatcg gcgtgatgtt ggttttcgtt gggatgggcg ctccgcatga
    29101 tgttttttcg caacagataa atatgaagtt tgagcatctg tccggcgatc agggtttatc
    29161 gcagagcact gtcggcgtga ttttgcaaga tcacaccggt ttgatgtggt tcggctcgcg
    29221 caatggtctc aaccaatatg acggatatcg ttttacggtg tacaaatcaa gtcctacgga
    29281 tccggaatct atttccgata attggatcac ggcattgtat gaagatcgaa atcataattt
    29341 ttgggtcggt accttaaata aaggtttgaa tcgttttgat cgggctcgaa aaaaatttaa
    29401 gcacatgatt tcggatcctg aaaatcaacg tacgataagt cataattcga ttacggcgat
    29461 tatggaggac cggcatggca atatttgggt gggcaccaaa aatggcctca acgtcattcc
    29521 ttacgattcg atgcgaaccg agactacaat gcggtttgtg cggtatttta gcagcagtca
    29581 agtagtcaat tccatttcac attcgtggat tacagctatc acggatgatg agaatggcga
    29641 tatctggatc ggtacgcagc agggacttaa taaaataacc aacggtgaaa taatacgtta
    29701 ctttgccgat aagcagggcg gttcgggact cccatccaat aatattacag ctttgtttaa
    29761 agacgctaat aataatattt ggatcggtac ggaagacggt gggctttgca aattagaccg
    29821 caagaacact cagaatcctg tttttgaaaa aatacctgtt cattccggta ctgatgcctc
    29881 tacaacacat gccgatattg taacggtgat ggtggatcgg caaaatatcg tgtgggtcgg
    29941 tactcgtttg ggattggatc ggatagattt taacagtaat accaaattat tttttcgaca
    30001 caaccctaac gatgtgtcgt cgttgtccga taatgaaatc cgagcgttat gtgaagaccg
    30061 gtcgggcaat ctgtggatcg gtacttacgg cggcggcggc ttgaacaaac tcgatttaaa
    30121 gccggtcaaa tttaatcaca tacgtcataa accggataat cccaacagct tgatcaataa
    30181 tattgtccgc tcgttttggg aggatagtga tgggattttg tggatcggta cgcacgacgg
    30241 actcaatcgt tatgatgccg gtaccgatcg ttttacggca tatcgcgccg gaccgggtcg
    30301 tctgagtagc aatgaagtgc gaaccattgc agaagataaa aacggaatga tctggctggg
    30361 taccgaaggt ggcgggttaa ataaatttga tcgccgaaca aatcggttta ccgtgtacca
    30421 acatcgtccg ggcgattcgc tgagtatcgc gggtaatgac atatccgtac tgttgagcga
    30481 taaatataat caattgtgga ttggaacttt cggcgacggt ctggataaat tgatcgtaga
    30541 aaacgggaaa gaaatatttg tccattatcg caatgtactt catcgttcgc agagtctgag
    30601 tcataatgag gtcagttcca tactgatgga ttcggaagaa aaaatctggg ttggcacatt
    30661 tggcggaggt ctcaatcgtt tggattcggt tcaggatcgc acgggttatg cttcctttaa
    30721 acgtatagcc gggcgagggg aaagtaattc ctcattaggc agtgatgtga tttttgctat
    30781 ttccgaagat agcgataaaa atatttggat cggcgccagc tcagggctca atcgtatcgt
    30841 acgcactacg gacggtagtt tgaagtttta taattattac gaaaaagacg ggcttccgga
    30901 taactacgta tatgcaatac tgccggacga acatggcaat ctttggatca gcacggtcca
    30961 aggtttgtcc cgcgcacgtc tgatccaaga tgagaacgac caatcggaaa agctgatgtt
    31021 tcgtaatttt gatgaaagtg acggtcttca gaataatgaa ttcaaatatg gcagttacta
    31081 taaaagtcgc agtggcgaaa tatttttcgg cgggaccaac ggttttaacc gtttttatcc
    31141 caaacttgcg atggacaatc cgcatatccc gcaaatcgta ttaacggcat tcaaaacaat
    31201 gcacaaatca gataaagcat ttgttgacgc ctatattgaa tctgtgtacc gccacgatcc
    31261 gattatcctc gattacaatg aagacttctc atttgaattt gcttcgcttg attttacaga
    31321 gccggccaaa aatcaatatg catacaaatt agaaggattt gataaaaact ggatccttgc
    31381 gggtacacgc cgttatgtga attatactaa cttagatgca gggaattatg tttttaatgt
    31441 acgcggatcc aataatgacg gcgtttggaa tgaacaaggt atttcgattc cgatacggat
    31501 tgttccgcct ttttggaaaa cgtggtggtt tatcgcattg agctttagtg cgcttatggg
    31561 gctgctgtgg gctttcatac gctaccgtgt gcgtgtgatc gaactccaaa aaaataaact
    31621 tgaagtcgaa gtgtccaacc ggacgcgcga aatcaaagca caacgcgacg atttgaaagc
    31681 cattaattcc caactcgaaa aaacgttgca ggaactcaac gaaacacaat cccaactgat
    31741 gcatgccgaa aaaatggcat ccctcggtca aatggtagcc ggtatcgccc atgagatcaa
    31801 taatccgctg acggttgtcg atggcaatct ccacctgctt gaagaaaacg tactgcgctg
    31861 ggatcacatt atcacagagt acgagcgact tattgagacg gccgaccctg agaaaattaa
    31921 aatgggtatg atggaaattc gcgaacggca tgagtacgaa tatgtccggc aggaaacgat
    31981 gaagattcta gactcttgta aaaacggtag ccaacgtatc aaacatatcg ttcaggattt
    32041 gcgtaagttt tcgcgtaccg atgcgatggg ttttatcccg gccgacttgc acgaggggat
    32101 tgagtccact ctgacggttt tgaaatccat gttgagcgac cgcataaccg tccataagca
    32161 gttcggcaca attcccatgc ttgattgtta tcccggtttg ctcaatcagg tatttcttaa
    32221 tattttaacc aatgccatag aagctattcc gaacgagggt attatcacta ttaaaacata
    32281 cctatctgaa aataatattc gtattcatat caaagataac ggtatcggta tgcatccatc
    32341 aatggttaaa agtatatttg atccattttt tacaacgaaa gaaattggta aaggaatggg
    32401 gctgggttta tccatcagtt atggtattat aaagaaacat aatggagaca tcgaagtgat
    32461 aagtgaagaa ggtaaaggct ctgaatttat tatcagcctc ccggttataa aacgcgtatc
    32521 ctgatgaaaa aacatatcgc tcaacttata gtttggattt gttggagtgg cacgattgtg
    32581 ctcgcccaat cggatcgtat taaattcgaa cgcttatcta tcggagaagg cctgtcgcaa
    32641 agtaaagttt tttcgattta tcaagaccac aaaggattct tgtggatcgg tacgatggac
    32701 gggctcaatc gctatgatgg ttacggtttt accgtttttc gccatgatcc gcgcgattcg
    32761 acgacgatat cagaaaacta cgtttggtcg attattgagg acaaagaaga aaacctgtgg
    32821 atcggtacta acggcggagg attaaaccga tttgatcgta agacgcaaaa gttcaaacct
    32881 tatcgtaaca tagccgacga tccgcactcg atcagtgata accgcgtgtg ggcattgtat
    32941 gtggatcgtg acggcgtgct ttgggttggg acgcgttacg ccggcctttg taagtatgat
    33001 cgtgcaacgg atcgttttat tgcatataaa aataacccgc aggactcgac aagccttagt
    33061 cataattatg tcatgacggt tttcgaagat tcaaaaaata atttttggat tggtaccaat
    33121 ggcggcggct tgaatagaat ggatcgcgct acgggaaaat ttacccgtta tcggtataag
    33181 cccgggcaac gttcgcccgt tcccgaagag cgcatatggg ctttatcgga agaccgcgaa
    33241 ggttatttgt gggtaggaac aaataataac gggttatcgc gttggaatgt agcaacgggc
    33301 gaatttgaga tattccgtca tagctctgcg gatcctaatt cgatcgcgga taattggatc
    33361 aaatcaattt attttgaccg tcaggatcgt ttgtggattt gcaccaatgg gggtgtaagc
    33421 atttatgata agacgactca aaagttctat aattatcgta ataaccctgc ggatccgtcc
    33481 agcatcagta ataattttgt aaacgccttt tgcgaagatc gcatgggtat tttatggttt
    33541 ggcacgtatg gtggcggtgt caatatgtac gacccataca aagaacgatt taagcattat
    33601 cacttggatc tttcgcaacc gcaagggctc agtggaaata ttgtgattag ttttgccgaa
    33661 gatcatgagg gcatcatttg gatcgggact gacgacgcag ggcttaatcg ttatgatccg
    33721 aaaaaacaga cctaccgcca ctataaacat aatcctgccg atgcgaagag tattagtaat
    33781 aatcgcgtat cggtgattta tgaagaccgt aaacataacc tatgggtagg tacgcgtggc
    33841 gggggactga gtcgttttga tcgcaagacc gaacaattta aaaattatcg aattgatcct
    33901 atatcatcgg cacaaagcaa taatgtggtt tttgccatat tggaagatac ctcaggaaca
    33961 ttatgggtcg gtacacgcgc gatcgggtta caaatctttg acgaaaaaaa aggtgaattt
    34021 cggcgcgtac gttacgacgg gcgaaatccg gcggacaatg gcgtccaggt attgatgcag
    34081 gatagccggg gatatatatg ggtgggaacg agcggtggcg gtattgtacg gctggatccg
    34141 aaaacgtatg cgtttacgcg ctttgtgcat gatccgtcca atccgaacag tctgagtcat
    34201 aatgccatcg tatcaatact cgaagacgcg caaggaaaga tttgggtcgg taccagcggc
    34261 ggattaaatc gcattgatcc atccaatgat aagattttac aaatcacaga gaagcatggc
    34321 ctacccaatg atgtcattgc ttcgatccag caagacggca atgggcaatt gtgggttagt
    34381 acgaataaag gtctgtcacg catcgacatc aataattttg aaaacggcgg ccctaccgcg
    34441 cacaatcgta ttcgtaattt tgatgtgttt ga
//
LOCUS       DAOQGF010000025        34288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_564_length_34288_cov_7.373061, whole genome shotgun sequence.
ACCESSION   DAOQGF010000025 DAOQGF010000000
VERSION     DAOQGF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_564_length_34288_cov_7.373061"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PK937_05505"
     CDS             complement(<1..887)
                     /locus_tag="PK937_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNH32058.1"
                     /translation="MLRPKLLDTLKDYNRERFIADLSAGTIVGVVALPLAIAFAIASG
                     VSPERGLYTAIIAGFLISFLSGSKVQIGGPTGAFVVIVYGIVEKYGIDGLIIATIMAG
                     VILILMGLAKFGGLIKYIPHPIITGFTSGIAVIIFSTQIRDFFGLTIDKVPGDFVEKW
                     ASYIEHGSSINFYALGIGIATLLIIIFWSRFSQKIPGSLAALIISTCVVYFFQLPVDT
                     IHTRFGEIPSSLPAPIFPNIQWSTITALIMPATTIALLGAIESLLCAVVADGMIGDRH
                     RSNMELIAQGLANIVTPFFG"
     gene            complement(907..1953)
                     /locus_tag="PK937_05510"
     CDS             complement(907..1953)
                     /locus_tag="PK937_05510"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNH32059.1"
                     /translation="MKKVKRWNALTFFKSTAVLFFFILLSACTPPSPIGPLPGKTPDS
                     FRIAFGSCNKTFLPQPLWNPIRAAQPDLWMWLGDIVYGDRGGIDTIRWMYNFQKNHPG
                     YAALRQTVPVIGIWDDHDYGVNDGGSEYEHKKETQRLLMDFLDEPAESPRRLQEGIYT
                     SYRYDKLNTRIKLILLDERSFREDPRANKDILGDIQWQWLYGELFGDTTDITLIASSS
                     QIIPNDNYAFNEVWQDYPASRDKMLSWLKTVPGTVLIVSGDRHYAEISQIENGRNNGR
                     STLTELTSSGLTHTKNYTLYSLLPIEPNSHRVGDFYGGLNFGVIDIDAVQRKIFGYVC
                     DHESMIRLQLVIDY"
     gene            complement(1960..2481)
                     /locus_tag="PK937_05515"
     CDS             complement(1960..2481)
                     /locus_tag="PK937_05515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNH32060.1"
                     /translation="MSSFFLRPSLLSDETAIYNLYNRVAAIRGGLAREAHEVTSDYVH
                     HFLSRSTATGLSIIAINENECLGEIHAYRPGIKVFDHVLSELTIAVDPKAQGLGIGKQ
                     LFTTFLDTVRTTMPNILRVELIARESNSKAIRMYEKLGFVTEGRFENRIRSVDGGLES
                     DIPMAWMNPNYKG"
     gene            complement(2481..3842)
                     /locus_tag="PK937_05520"
     CDS             complement(2481..3842)
                     /locus_tag="PK937_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNH32061.1"
                     /translation="MQSIQTILRRLTEAPDKEALYWNDRFFSYAELNTAIAAWEPVLK
                     DNGVGRGTVCAFLGDYSPQTAALIFALMKVKAVMVPFTYAIQHEIEEFKNIAGVECMF
                     RFAPDDTWTFERYNAVPRNALIENFMQRDVPGLIVFSSGSTGKPKGILQDCERVMHKF
                     VEKRPGWRTLLFLMMDHFGGFNTFLSSFAYGGTAVCLSDRNPDTVCRIIQTSRATLLP
                     TTPTFINLLLASRVYGQYDLSSVKMITYGTEVMNAATLSKVRDIFPNAQIKQTYGLSE
                     LGVLRSQSESDDSVWVKIGGAGFEVKIVDNILWIRSEANMVGYLNAPSPFDNEGWMCT
                     GDQVEVRGEYMRILGRKSEMINVGGQKVFPVEVENVLMEAGNVREVSVFAKPHPIMGH
                     VVHAKITLHEPEDTLLATERLRKFCLERMAKYKVPVKFVFVDNSEQHNARFKKIRRDS
                     ESS"
     gene            complement(3847..4092)
                     /locus_tag="PK937_05525"
     CDS             complement(3847..4092)
                     /locus_tag="PK937_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNH32062.1"
                     /translation="MAKTTHDIKKVMARIFEVDPSSLHDTIRQDEVENWDSLRHMNLI
                     LGLEEEFGVEFTPEQSVEILSLPLIKVTLQELGVTFS"
     gene            complement(4094..5875)
                     /locus_tag="PK937_05530"
     CDS             complement(4094..5875)
                     /locus_tag="PK937_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNH32063.1"
                     /translation="MMIPERLTDITLPDTLSETIALVNHLDHLEKKPEQSVRIHILRN
                     FTIEGIEPFLKLYHYQSGIRPEISFGNYDNVRQELLDKNSSLYQRHPDIVVLGLVLET
                     LDTNLRTFGWTADRVISEIESLLDTALQNTQALLAVNTFVPPWDSEWGMYRSPDAAER
                     FEEVQRINRKIRERVRRDPTRLYLMDWERYLIRLGEEAAMDYRFWYTSKAPFKKDFMS
                     AYAFDIAKLSRGLKGKTKKCLVLDCDNTLWGGIIGEDGVSSIKLDRDSFPGRVYRDFQ
                     LNALALYERGILLTLCSKNNEADVWEVFDHHPDMALRREHIAAWRINWTDKATNIRSL
                     AEELNIGLDSFVFVDDSPAECELVRTMIPDVTVLQVPQKLYTYPPFVIRSGFFDALQV
                     SSEDKNRTRMMHEESMRRVEETRYTSIDDYLASLQLYATIHEAPTDEIPRVAQLTQKT
                     NQFNVTTRRYSEAQITAFAENPDASVWTMRAGDKFGDFGLTAVIIVTRNGDTAEVDSF
                     LLSCRILGRKLETAFVRHVMAALTPRWNIRTWQAYYIPTKKNGQTEKFWDSFGFRPTP
                     QADRSVKYTIDASALDTTPIPFISYRS"
     gene            complement(5872..7059)
                     /locus_tag="PK937_05535"
     CDS             complement(5872..7059)
                     /locus_tag="PK937_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH32064.1"
                     /translation="MPDYTQIQIGDTAEIRHTITASDIEKFVALTGDDNKMHVDENFA
                     GRTSFKKPVVHGMLGASFISTVIGTKLPGDGALWFSQSLEFLLPVRIGDEITVRAEVL
                     RKIDAEQIIELQTDIINQHKQKVTTGKAKVKVIAQEKPSVAEETTVRKKVALIIGATG
                     GIGQATCRMLAKQGFDVAVHYFSNEKRAFDLVVSVKNEGRAAHAFKADIFKPEDIHHL
                     AEDVTRKLGTVTVLVNAATVKIPAIKWASVAWDDISQQWDINVRASFYLCKGIIPLME
                     KQKYGKIILLTTMYTESTPPTELMGYVTAKYALNGFAKALAVEMASKNITVNLVSPGM
                     TDTELVADVPEKVKLLNAMKAPMKRLAQSKDVAGTIAYLASDAADYITGETIRVNGGQ
                     TML"
     gene            complement(7109..8092)
                     /locus_tag="PK937_05540"
     CDS             complement(7109..8092)
                     /locus_tag="PK937_05540"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNH32065.1"
                     /translation="MGIGITGVGKYIPSKEITNEQVEAWTGVSAADIVSKIGVTKRFV
                     VEAHETASGMSVEAARQAMVMAGITADQIGLIICATFTGDYLCPAMACRVQEQLGAKN
                     AGAYDVMANCTGFQIGLANAADRMTLDSSIAYALVIGTALQSRFINWKDPNSAIYFSD
                     GAGAAILARVPEGYGVLASDIMANGKVYDAVRVRGGGSSYPLRTENVNDVLPYFELNG
                     LEVWKQVVQFQPRVIMRVLEKISKKTDAVDFFIFHQANLRLIEFLMAKMKHPMHKTQT
                     NVETIGNTGEASMAIALCDAVLAGKLQRDQLVVISGVGAGFIFGATVLRWY"
     gene            complement(8113..8895)
                     /locus_tag="PK937_05545"
     CDS             complement(8113..8895)
                     /locus_tag="PK937_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH32066.1"
                     /translation="MPRPKAPASQAKSLIWVLWTDLGRKREYGMEGIYEYILDGFKIT
                     EVVTDPLWKENCYLITSASGEQALIDPGYDADRLADLISQNGDRGLTWVLLTHAHHDH
                     VGAVTPICRMFSIPAYLHKDDHKLIRHAPMYAIRFGRQPIPMPQPVNFFETPHVFELG
                     ALALRALHVPGHTKGGAAFVLGNALFTGDTLMHKHVGRADLPGSDMTMLKQSVDKLLS
                     EHHDTARIFAGHGKPWTIGEAREWWASAKNEPGVHDHFDTFA"
     gene            complement(8834..9538)
                     /locus_tag="PK937_05550"
     CDS             complement(8834..9538)
                     /locus_tag="PK937_05550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH32067.1"
                     /translation="MATPVMIITGTSKGIGKGLAEYFLNKGYRVFGCSRSEATITHAA
                     YHHTALDVSNESAVRLWIRTVKKEAGRIDILVCNAGLVESALQMTVTPGDVLEAYLKT
                     NVAGTYYVCRETAKTMLLQKQGRIITISSIMTRLHAPGTSVYSATKSAIEQMTKVLAR
                     EMAPLGITCNVVAPSMSPTDAAKSFGPEWEKNMLAQQTIQRPITTDEIANVISFYAAP
                     ESACITGQVTYMGLVD"
     gene            complement(9543..9842)
                     /locus_tag="PK937_05555"
     CDS             complement(9543..9842)
                     /locus_tag="PK937_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32068.1"
                     /translation="MNTEQILNVIQESFDSLTRSGLIASQVQVTPDLVILGNGSPLDS
                     MAFVTLFTDLEERIATASGKDIFLVLDEIEGFNINNPFLSAETIAGYIVGLIQKG"
     gene            complement(9923..10747)
                     /locus_tag="PK937_05560"
     CDS             complement(9923..10747)
                     /locus_tag="PK937_05560"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNH32069.1"
                     /translation="MEKYFLFSIDLEDVRFWMSDGKKYRERVPAMTERYLKFLAQHRM
                     TCTFFVVGDVAEAYPSLIREIYSAGHEIACHTHRHIPLDQQTPETFRDDLLRNMDGLY
                     KAGVETLNGFRAPIFSLTKKTPWAYEILIEQGFTYSSSVLPATNPLYGWPEFGATFRR
                     VHDRIWELPITLFPSRFISVPCAGGLYFRTLPLWLTARAFRHHWNQSRPVLSYFHPYD
                     IDTEQERFMHPGLKDNRFYNWVMYQNRGTMLDKINTILADGATIIPYRDYITRHLQ"
     gene            10922..14080
                     /locus_tag="PK937_05565"
     CDS             10922..14080
                     /locus_tag="PK937_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32070.1"
                     /translation="MLPFFIRHTLFWWVFVSHLFAQQYYIKNYSTSDGLPSNTIFEVF
                     VDCDYAMWIGTVDGLSYYDGQTFQNFNKTQGLINHRVWSVAQDSSGNIWVGTRTGINR
                     FHKTRSGWQIRAWLTQLSVRHIHIETDQSMILSTENGAYKFTCFTDDSSSLVPIEGLQ
                     GKMVYQTLRDPDNRLWIATDQGVWIQKGTVLENISTPFPIVRGLFLRDSSHLYALHFR
                     GGISLTDPRSDSPSFVSQDLPKGFTKRSFYRMIVDPTGNFWAATDLGITRLRFSDTRV
                     VSMEVIDETKGLANNLTIPLVSDHEGHIWVGTHNGLSRFAGLRFSYFIPHNNPGIQVY
                     KSIAASGTDIYMLTSAGLFYVDAQHPAVPLPLYGNTPIQEGVSLGKNRLALVNDSEIA
                     IFFVGGKTFRKQKKISVEGQSIASFLFLDEQDVMIGTSAGLYRHRHGRVTRVNASTFT
                     DQMMVYTLMRDDKGAIWVGTSHGAYVLTADQDSLRVQSWSLPGVMPFQAVGQIYRDAS
                     GMMWFCTEGAGLARWDGQNVRVYTTDNGISNNIVYSVFEPDTTTLWVCTAGGIDVMNR
                     KNDSWMHWDSHSGFFRKGSTPYSIMKSSDSVYWISTSRGLVRTDFSEPFASGNSVPVL
                     VDQILVQGKPQRHSGILRIEPGVQQIQFRIKAISYRYENDVRYSFFMEGFDEAWSEPS
                     SQGVIQYAGLSPGIYRLKSKAITPGGQYRGPDVVLEVLTPFWKETWFVAISCLVLIAV
                     TAGAYRWRTYHLWKKARQLEMLVRDRTKDLVRKSRDVIKQKKELEKTLQTLRTTEAQL
                     IQAEKLSALGELTAGVAHEINNPIAFIYGNLPLLEKDLRRLTELAQKAKTGPLDDKEA
                     LEMTQIENDIHSAIVSTRHGVQRVQVIVKTLKKFVRLDEAKEDFKSVEECLDSAADAI
                     IIQYPKVQIKKDYRETPQILCYPSELQQAFYHVMLNAIQSQATDARVDFSTSTDGKYV
                     YVGIRDYGTGIPEAISQKIFDPFFTTKDVGRGKGLGLSMTYGIMEKHEGGIRFENCQD
                     QGAKFILMLPVERRSSSPSVLS"
     gene            complement(14147..15268)
                     /locus_tag="PK937_05570"
     CDS             complement(14147..15268)
                     /locus_tag="PK937_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNH32071.1"
                     /translation="MSTHAKILVVEDDFQNRQLIQIYLSRMNFDVTLAPNGFAAMEKL
                     KTYKPDLIISDISMPYMDGFELYNNVKAIPDLRSIPFIFLTAHSDADKRRYGKEIGSD
                     DFLTKPIEQEDLVASIRGKLKRVEEIRSSTEAEMLTQIDQLKREILSTITHEVNTPLF
                     IIKLTANLLLDETMEFKPHELQELLLRIKKSGERLDALLKDFLITVRIAAGDAKSEYK
                     EAKQDSDINFIVEHLVPQFKKRAEIKQLRIQSQLERHLPGLQLHVDQVADVVERLFSN
                     AYKFTPPGGEIRIRSGKDHDYVRLDIEDTGIGIPEEQLDRIFHKFYQVNRAEMEQQGA
                     GLGLAIARDLTEINGGRLVVKSHEGMGSTFSLLFPIHVI"
     gene            complement(15273..17405)
                     /gene="metG"
                     /locus_tag="PK937_05575"
     CDS             complement(15273..17405)
                     /gene="metG"
                     /locus_tag="PK937_05575"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNH32072.1"
                     /translation="MPTDTSFKRILVTSALPYANGPSHIGRLTGAYLPADIYVRYQRL
                     MRRDIVYICGSDEHGVPITIQAEKEGVTPQALVDRYHAMIQDEFSRMGISFDHYDRTS
                     NPVHHDTAQEIFLDLLEKNLLKRKPEMQWYDEQAKMFLSDRYVEGTCPVCQNTDARGD
                     QCEKCGTYLNQMDLIQPRSKVTGTTPIAKETTHFYLPMGEFQSELEKWISTKSHWKDN
                     VINYCKGWFAQKLGDRAITRDIDWGVPVPKKDRKNNAIEGSDRKVIYVWFEAVLGYIS
                     STKEWAKKVGQPEKWRDYWQKDDTQLVHFIGKDNIVFHAIMFPIITMAYNQSKTSGRL
                     VLVSDVPACEFLNLEGNKLSTSRNYAVWVKDYLQKFPPDLLRYTLTSNLPENKDSDFS
                     WKDFQIKNNSELADILGNFINRSLSFTIRNYHNKVPSRGTLTAMDEQMLSEILAAQTE
                     VGKFIEEFRFRDALKRFMDLARAANKYFNDTEPWKKIKTEPERGATILWVSVQVVRAL
                     AVIMHPFLPFTSEKVWQMLGLSGLVKEQDWNTIGTSRMDTGHVLGKVEIVFPKIEDDV
                     IQKEIDHLNSMIEQKTTESAQSKPVEKPKSENEAVTAAQISIDEFGKIDMRSAKVLHA
                     EKIEGTTKLLKLQLDLGFEKRQVVSGLAEYYKPEELIGKDVIIVANLKPAKIRGVESN
                     GMILTVSDGTTLKILSPEAVMPGGLRVK"
     gene            complement(17448..18263)
                     /locus_tag="PK937_05580"
     CDS             complement(17448..18263)
                     /locus_tag="PK937_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH32073.1"
                     /translation="MGLEVTFYGTRGSISATHEDSLKYGSHTACVVISSGERSVIIDA
                     GFGIGFYSDHLKNKMGEFHLLISHFHWDHIQGLNYFGAMHHPKSINHFYSPQSNDKLK
                     EVMDIYFDGSYGPFNGWQQLNSKLEFHQLTQDIEINGFQISCRALNHTDPCFAYKISD
                     GGHSVVYASDHEAIDNSVNREFITWAKGADMLIHDAMYLDEEYAQRVGWGHSTFDMAC
                     RNAQQIECKHLLLTHHEPMRTDRELDRIELRLKEKYSDLRLTCAQQEVVYVTK"
     gene            complement(18254..19156)
                     /locus_tag="PK937_05585"
     CDS             complement(18254..19156)
                     /locus_tag="PK937_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH32074.1"
                     /translation="MKFTLLGTRGSRPILTPDRVRYGGNTTAFKITIDGMQPIYIDGG
                     TGIYREGVTISEQHPEGFHAHFFITHTHWDHILAFPLFAPLFNKNTKISISGPRSEEY
                     TIEELFKLQHAKGLIPIPFSLVRDKITFHELHPDQTIPLENATVKTVQLNHQGLTLGY
                     RIEHGGKSVLIITDHAPIENNHLGVLMKAWNKEDFTKTEKEFYENMVRFCTGADIMLH
                     DTHFTKEGIKGKENWGHSTPEMAVDLAVKAGVKKIILGHHAPEDTDTFVEQKITSAND
                     YVKSIGKAGAIEVVALREGTEVWV"
     gene            complement(19274..20848)
                     /gene="pckA"
                     /locus_tag="PK937_05590"
     CDS             complement(19274..20848)
                     /gene="pckA"
                     /locus_tag="PK937_05590"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNH32075.1"
                     /translation="MTTNNELAHLGIVNTGTIHKNLSVSQLVELAIKRGEGQLASNGA
                     LVTNTTPHTGRSPKDKYVVEEPTSKDKIWWENNKKVSQAQFDVMFNRMRSYLQGKDVF
                     VFDGFAGADTKNRLSVRVISEKAWHSLFAKTLFIRPTEAELATHHADWTVIDVCGMHA
                     IPEFDGLGTKTFIMLNFEKKIVLIGGTHYAGEIKKSIFTVMNYILPERGIFPMHCSAN
                     SNKEGEVTLFFGLSGTGKTTLSADPLRRLIGDDEHGWSNEGVFNFEGGCYAKTIKLSK
                     EGEPQIWNAIRFGSVLENVVLKQDGTPDYDSAAIAENTRVTYPVEYIDNCIIPGIGGH
                     PKNICFLVCDAYGVLPPISKLTPAQAMYHFLSGYTAKVAGTEAGVTEPTPTFSTCFGA
                     PFWPLHPNVYAELLRKKLNEHNATVWLVNTGWSGGGYGVGSRMKLSYTRALINAALSG
                     KLNNVKYNNDPVFGVAVPESCPDVPAEILVPKNTWKDKAGYDAQAKKLADLFIKNFEK
                     FADKASDDVKKAGPKA"
     gene            complement(20888..21877)
                     /gene="ricT"
                     /locus_tag="PK937_05595"
     CDS             complement(20888..21877)
                     /gene="ricT"
                     /locus_tag="PK937_05595"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNH32076.1"
                     /translation="MQNPIQVEFKANRKEIFLNKNNLVVKVGDHVIVQCENGVDMGKV
                     ALTHEQIPEDLTKDKELRQFIRVANPDEISKLADNRNEEEKTKAVVKQKIIKHGLKMK
                     LVDAEYQFDRKKIVFFFTAEKRVDFRSLVKDLAGEFKTRIELRQIGVRDEAKRVDGYG
                     PCGKQLCCSGHLTEFQPITTEYAKDQGLQLNPSKLTGLCGRLKCCLAYERDFYVNTLS
                     RFPGVGSPIKTKKGRGEIKHLDIIGNKVHIKQDHDNEWETLTLEDVADILGIDMSDIH
                     GCGSGCASGGDGCQSGKCNTHDHVEEGTMQNPLKDFMENDPFFDQYNFPRPKI"
     gene            complement(21923..23062)
                     /locus_tag="PK937_05600"
     CDS             complement(21923..23062)
                     /locus_tag="PK937_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNH32077.1"
                     /translation="MLSKVIGQEAAKKQILTAFANDRMAHAYLIAGDEGLGAEELALE
                     ATRFILCKHPDRERWEPCGECANCKKMATLQHPDVHYYFPVMRATEEDEQRRLLEEKT
                     RNLYARVRIANGSIPIGDPENPEVNSIRWLSRAISLRSFEGELKIFILTFIEDMHAEA
                     TNAVLKILEEPPASTLYFLTTTNIHSLLPTIISRCQIVKLHRLTDDQLLDGIRRHYDV
                     AENQAKFISRLANGNYYQASELISGDFPRKREAMLEFLLAAMSNKSASIQTIIEQWNE
                     EAEKDKSFYTDILTMLITWFQDVVYMRELGEDKSLIDRFLINEDKTERLEKFIKAFPR
                     VRVEEALSEIEKAVDLIGRNIYINLILMNLGLNLRSIVNPSSGKS"
     gene            complement(23249..26056)
                     /locus_tag="PK937_05605"
     CDS             complement(23249..26056)
                     /locus_tag="PK937_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNH32078.1"
                     /translation="MQRRTFVLLFLSLSLTSLFAQTDETIPMDPAVRSGVLSNGLRYF
                     IKTNARPEKRVELRLAVNTGSIMEDDDQLGLAHFVEHMAFNGTTHFQKNDLISYLQSA
                     GVKFGAHINAYTSFDETVYMLTLPSDKQDIIHNGFLILEDWAHGITFDSLEIEKERGV
                     VIEEWRTGIGAQQRMSDQYWPKLFYGSRYAERMPIGKKEILEKFSHASIKRFYKDWYR
                     PELMSVIAVGDIQPDSIEYLIRKHFSNIATSDAKKVRPRNDFPVPPHKETWTAVARDK
                     EAPNTMAQIFYKHPSQTVRSVQDYRTSLLRMLYTGMLNKRMEELTQQANPPFVFGGAF
                     YGNFVRATDVYAVYAGTGETALERGLLAVLIENRRAQQHGFSRDEMEDYKKEILSNYE
                     SSYNNRDKVLSSSLADEFVEYALTAEAAPGMAWEYETVKRLLPTITVDEIHALAAQWI
                     TTENRIGVVTAPQNETVKLPDEARLLALIDSVATIQTVPYVYKTVQVPLLATLPHPGK
                     IVKTKKLKEIAAEEWTLSNGMKVILKPTTYMTDEIKFSAYSFGGWSLGAPSQDMSAIY
                     GMAPLGEGGIAEFSKTDLEKILSGKNVSVSTGIRMLSETISGQSLKKDMETMFQLIYL
                     NFTAPRLDTTVFYALLSRQMAYIKNLDNDPRFVFSNELNRVLSRDHPRDPSVLRRERF
                     SEINPQTALAIYRDRFAEAGDFVMTIVGSFEIETMRPFVERYLAGLPTTGRKETFKDL
                     GIRRPGRNMDTTIYRGVDPRAQVNMQFHGALKKYDDLFLLRATAMLLTNKLIENLRED
                     KGGVYGTSANAYANKYPVSNYAVIIGFSCAPERLDSLIGAVHEEIRKLAENGPAESDV
                     NKIREALRRDLERNQKENAYWLRWIENAYYENASIKNITPEAQEKLIRRLSAKDIRKI
                     AQKYIQNGHALIFKMRPEEKK"
     gene            complement(26091..26990)
                     /locus_tag="PK937_05610"
     CDS             complement(26091..26990)
                     /locus_tag="PK937_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH32079.1"
                     /translation="MKITAFFLFVSFSASQLFAQAAESKTTVAVLPPDITKIVTSAAT
                     GSSEVNVIADALTQKIVSTLVGLKRIAVVERAAMDKILSEQDFQMSDLAAENTPVRLG
                     ELMGADFLVILHLYQAQTTLVTQTEEEKQTWGDRGTQYSGLLEYGIRLVQVSTGEVTT
                     TKNLKGDTGFLAYTTTTEALSNALNMSSDKLKDWLKSSFPVRGKIFEILKEKKGEAVS
                     VAITCGSASGVRKNDAFKVVEYTEVELDGKKVKRSKDIGKIKVSKTESDGIFSRCEVI
                     NGGKTITSKMKSGSQLDVILVKD"
     gene            complement(27020..28534)
                     /locus_tag="PK937_05615"
     CDS             complement(27020..28534)
                     /locus_tag="PK937_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH32080.1"
                     /translation="MTSQSSQHHKETREVARGAFINLIGNFGKLSHFAFDLIATRVLG
                     QTLFGYFSTTWLLMHLSFIVCYFGAHRLVIDFVVRYKDTDEDEYYKSILGYIILSLGL
                     SAFLVLNVYLFADHLAAYMGKPPVADYMKIMVWCAPFYCLTTILMTATRGLKIMKLWV
                     FIRNGFEPLVDLILLLVVFFVFGSIAAPFWAKMIAFTLGSGLSIYYFRKHFSMRRIFS
                     HLPDGSTWKRIFSFGFPVMFADFISVVILKVEIIPLSILVPSAQVAVFQVILNTGNTM
                     RNIPQAIDPIMMPVVVAMRQRNDMRALEGIFATLIRSSLFLACGFFVLTTIYGDFLLR
                     IFADEFAYAAGALSIVCFGMMIHTVTSNIEPVLVMSGYPYLNLFNNVFLVAVSMTLDF
                     ILIPTYGIFGAAIGNLTGCLLTAALQIAEVHLILKIRPFRRDLLHIVWIGLGCYIIFK
                     GLDFLFPAWTDLLIYDIICVCIFTAFYLYVGWKLFLHDEERILFSSMIHKPQSE"
     gene            complement(28689..30032)
                     /locus_tag="PK937_05620"
     CDS             complement(28689..30032)
                     /locus_tag="PK937_05620"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32081.1"
                     /translation="MKSMFKSLHLVFMSLAIMAMLGCEEKTNSNQEIKSGGENFNLFT
                     GIDGTEIENLRSEYSKTFQLSNGKVVTRFSAQKVHELVNGQWIDIDYNKKPEKSAGII
                     EGSITGTLKYTRISNNITTTEYAPIFDNLGSYPGYLPDDQSVTYNNENSRLVVTFNGI
                     NNIPSGYRVRSSELALSNSIPTSGSVSRSEIYGDIDIKLFTSNSLYGTQVHYDACSVG
                     TTLGTKKTGFFPVILDNSSLNNAVINAIPNNRVSFSLVSVGALCSITYTHLDIYLELI
                     PRYSITTVVNPSGSGTISLSPAPGADGKYEQGTLVTATASPSSGWQFYNWSDANNSQS
                     NQISFTVNSNASLNANFIKQLQINGPVELPTNGSATYTVTTIPDATTYKWYKLVKQTT
                     YPYGTTYSYQSTGTSYVGVMGTGAIPLVLIMKVEAYDASNALRGSGIIAVEASEN"
     gene            complement(30434..32914)
                     /locus_tag="PK937_05625"
     CDS             complement(30434..32914)
                     /locus_tag="PK937_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH32082.1"
                     /translation="MKNLTKKLILTSLMALMAFSSAFGQNGTLRAKFVKSTDGSPVTD
                     ATFTAVLASNNQSYGVRTPNAQGEMEWSLPPGNYYVKRQTGNYVATNSVTVTLLAGET
                     NSTLVTNPTSLTPLAFLRAKFVKSTDGTPVTDATFTAVLTSNNQNQGTRTPDAQGEME
                     WALPPGNYYVKLLTGYYVATNSVTVTLIAGETNSTLVTNPTSLTPSTVLRAKFVKSTD
                     GSPVTDATFTAVLASNNQNYGVRTPNAQGEMEWVLAPGNYYVKPITGNYVSANSSTVT
                     LVAGETNSTLITNPTSLTPLATLRAKFVKSTDGTPVTDATFTYVYSAGNYSGTLYPDA
                     NGIITQLLPPGDFKVKTLSGNYVGYTSGTTTVTAGEINAAFVDTPRVLTPLGVKAHFV
                     NSNNTAVISGKTVKITDGSNNTVATQTTNANGDVYIQLTPGSYTMTAFDNDANYQTQN
                     IPITIPSNAVLDKGTINMAQNKFALAFNDAGDYVNAGQESSLNTISSFTIEAWVLRKS
                     GGNSQHIISKGGGWGNNGVNIHFYYDPTPNPNPASTSLRVDFRGTNPAGWFFYNVDDQ
                     VGFDQWHHISIVWDRTTLTTPRVYVDGIYEHVGTGSPYSGYIYTDNYDINIGRNSSIN
                     SAYFKGNLDEVRWWNFARTQSEIQASMNTHLNGNESGLVLYYKLDEGSGSLINDIAGT
                     NSQGTLMGNTSWLTTTFPHISGTMTKTLAGTFYESAYTDTTGEMQKDLSTIPMEFGLE
                     SNFPNPFNPTTTLRYALKEDVRVSLKVYNLLGQEVRNLVNEHQTAGFKNVLWDGKNNQ
                     GKQVPSGVYIYRLEAGSFKMSKKMNLIK"
     gene            complement(33255..>34288)
                     /gene="ettA"
                     /locus_tag="PK937_05630"
     CDS             complement(33255..>34288)
                     /gene="ettA"
                     /locus_tag="PK937_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNH32083.1"
                     /translation="IAVTHDRYFLDNVAGWILELDRGEGIPFEGNYTSWLEQKKKRLE
                     VEEKQESKRQKALAEELEWVRMNPKGRHAKSKARISAYEKMVNEQNEKRNEEMEIFIP
                     PGPRLGNVVIEANGVGKSYDDRLLYENMNFSLPPGGIIGVIGPNGAGKTTLFRMITGG
                     EKPDAGSIRIGETVKLGYVDQNRPLDPNKSIWEEISGGEETLMLGTREVNSRAYVARF
                     NFSGSDQQKKVSMLSGGERNRVHLAKMLKEGANVLLLDEPTNDLDVNTLRALEEALLE
                     FAGCAVVISHDRWFLDRVATHILAFEGDSQVVWFDGNFSQYHENFKQRMGIDADRPHR
                     IKYKPLMRG"
BASE COUNT         8927 a         7582 c         8116 g         9663 t
ORIGIN      
        1 ccgaaaaaag gtgtaactat attggcgagg ccttgtgcga tgagttccat attggagcga
       61 tgtcggtcac cgatcatacc gtcggcaaca acagcgcaga gcagtgattc gatcgcgccc
      121 agtaaggcga tcgtcgtcgc gggcataatg agggctgtga tcgtagacca ttgaatattg
      181 ggaaaaatag gcgctggtaa tgaagacgga atttcaccaa agcgggtgtg aatcgtatct
      241 acgggcaatt gaaaaaaata aacgacacac gtcgaaatga tcaaagccgc caatgagccc
      301 ggaatttttt gtgaaaagcg tgaccaaaaa atgataatta gtagggtagc gatgccgata
      361 ccaagcgcat aaaaattgat agaggatcca tgttctatat acgatgccca tttctctaca
      421 aaatcaccgg gtactttgtc tatcgttaag ccaaaaaaat ctcgaatttg tgttgagaag
      481 atgataacgg cgataccgga tgtaaaaccg gtgatgatcg gatgaggaat atatttgatc
      541 aggccgccaa atttagcgag ccccataaga atcaatatca cgccggccat gatcgtggcg
      601 ataatcaaac cgtcaatgcc gtatttttct acaataccat atacgatcac gacgaatgcc
      661 ccggtcgggc cgccgatctg aactttgctg ccgcttaaaa atgaaatcaa aaaaccggcg
      721 atgatcgccg tgtataaacc gcgttccggt gaaacgcccg atgcgatagc gaaagctatg
      781 gcgagcggca aggccaccac gccgactata gtgcctgcgc tcagatcggc aataaagcgt
      841 tcgcggttat agtctttcag tgtatctaat aatttagggc gtaacataaa acctgcgcat
      901 gtgtttttag taatcaataa ccaattgtaa acgtatcatg gattcgtgat cgcacacgta
      961 accgaaaatt ttacgttgta cggcatcaat gtcaatcacg ccaaaattca gtccgccata
     1021 aaaatcaccg acgcggtgtg aattaggttc gatcggcagt aaactataaa gcgtgtaatt
     1081 tttggtgtgt gtcaaaccgc tggacgtaag ctccgtcagt gtcgagcggc cgttatttcg
     1141 gccgttttcg atttgagata tttcggcata gtgtcggtca ccgctcacaa tgagtacggt
     1201 gccaggcacc gttttgagcc aggataacat tttgtccctt gaagccggat aatcttgcca
     1261 cacttcattg aacgcgtaat tatcgttggg gataatttga ctggatgaag cgatcaatgt
     1321 gatatccgtc gtatctccga aaagttcacc gtacaaccat tgccattgta tatcgccgag
     1381 tatatccttg ttggcgcgcg gatcttcacg aaatgaacgt tcatcaagaa gtatcaactt
     1441 tatgcgggta ttgagtttgt cgtagcgata cgatgtataa ataccttctt gcaagcgccg
     1501 ggggctttca gcgggttcat ccaaaaaatc catcaataga cgttgggttt cttttttgtg
     1561 ttcatattcg ctgccgccgt cattgacacc gtaatcgtga tcgtcccaaa ttccgatcac
     1621 cggtacggtt tgtcgtaacg cggcgtagcc cggatgattt ttttggaaat tgtacatcca
     1681 tcgaatggtg tcaattccgc cgcgatcacc gtaaacgatg tcgccgagcc acatccaaag
     1741 atcgggttgg gcagccctga tcgggttcca tagcggctgg gggagaaatg ttttattgca
     1801 agaaccgaaa gcgatacgaa atgaatccgg agttttgccg ggcaaagggc ctatcggtga
     1861 cggtggtgta caagcagaaa gtaaaatgaa aaaaaagagg acagcggtgg atttaaaaaa
     1921 agttaaagcg ttccatcgtt ttactttttt cacgaagggt caacctttgt agttaggatt
     1981 catccaagcc atcggtatat ccgattccaa accaccatcc acactgcgaa tacgattttc
     2041 aaaacgacct tccgtcacga atccgagttt ttcgtacatg cgtatggctt tagaattact
     2101 ctcgcgtgca atgagttcta cgcgcaatat gttcggcatg gttgtgcgaa cggtgtctaa
     2161 aaaagtcgta aagagctgtt ttccgatacc cagaccttgt gccttcgggt caacggcgat
     2221 cgtgagttca ctcaaaacat gatcaaatac tttaatgccc ggtctgtagg cgtgtatttc
     2281 accaaggcat tcgttctcat tgattgcgat gatggataaa ccggttgcgg tgcttcgtga
     2341 tagaaaatgg tgaacataat cggaagtgac ttcatgagct tcacgagcaa gtcctccgcg
     2401 tatagcggca acccggttat aaagattata aatggccgtt tcatcgctca acaaagaagg
     2461 acgcaggaag aatgaactca ctaggaagac tccgaatcgc ggcgtatttt tttgaatcgc
     2521 gcgttgtgtt gttcgctgtt atctacaaaa acaaatttaa ccggtacttt gtattttgcc
     2581 atacgctcga ggcaaaattt gcggaggcgt tccgtagcga gaagtgtgtc ttccggttca
     2641 tgcaaagtga ttttggcgtg cacgacgtga cccatgatcg gatgaggttt cgcaaaaacg
     2701 ctgacttcgc gtacattgcc ggcttccatt aaaacatttt cgacttcgac gggaaaaact
     2761 ttttgaccgc cgacattgat catttccgat ttgcggccga ggatgcgcat gtattcgccg
     2821 cgcacctcaa cttgatcgcc ggtacacatc catccttcgt tgtcaaaagg gctgggcgca
     2881 ttgaggtaac cgaccatatt ggcttcagac cgaatccaca aaatattatc aacgattttt
     2941 acttcaaaac ctgcgcctcc gattttgacc cacacggaat catcactttc ggattgggag
     3001 cgcaacacgc cgagctcgga aagcccgtaa gtttgtttaa tctgcgcgtt aggaaaaata
     3061 tcgcggactt tcgaaagagt tgcggcattc atgacttccg taccataagt aatcattttg
     3121 accgaagaca aatcgtattg cccgtacaca cgggatgcca aaagaagatt gataaatgtc
     3181 ggtgtcgtgg gcaagagtgt cgcacgtgaa gtttgtatga tgcggcacac ggtgtcagga
     3241 ttgcgatccg aaagacaaac ggccgtacct ccgtaagcaa acgatgataa aaaagtgtta
     3301 aatcccccaa agtgatccat catcaaaaaa agaagtgtac gccatcccgg tcttttttcg
     3361 acaaatttat gcatgacacg ttcgcaatcc tgcaaaatac ccttaggttt gccggttgat
     3421 ccgcttgaaa aaacgatcag ccccggtacg tcgcgctgca taaaattctc aatcaacgcg
     3481 ttccgcggaa cggcattgta cctttcaaag gtccatgtgt cgtccggagc aaaacggaac
     3541 atacattcga caccggcaat gtttttgaat tcttcgattt cgtgttggat cgcatacgta
     3601 aagggcacca tcacagcttt gactttcatc aacgcaaaaa tgagcgccgc cgtctgcggt
     3661 gaataatcac ctaaaaaagc acaaacagtg ccgcggccga ccccattgtc tttcaaaaca
     3721 ggttcccacg cggctatagc ggtgtttaat tccgcataag aaaaaaaacg atcgttccag
     3781 tacagcgctt ccttgtcggg tgcttctgtg agtcgtcgga gtatggtttg aatggactgc
     3841 atacgattac gaaaaagtaa ctcccagttc ttgtagtgta actttgatga gcggcaaact
     3901 cagtatctcg acgctttgtt cgggggtaaa ttctacgcca aattcttctt cgagaccgag
     3961 tatgaggttc atgtgccgta aactgtccca gttttcaact tcatcctgac gtatagtgtc
     4021 gtgcagcgat gaggggtcta cttcaaaaat ccgtgccata acttttttta tgtcatgggt
     4081 cgtcttagcc atattaactc ctgtaagaaa taaacggaat gggtgtcgta tccagtgccg
     4141 acgcgtctat cgtatatttc acacttcgat ccgcttgtgg cgtcggtcgg aagccgaagc
     4201 tgtcccaaaa tttttcagtt tgaccattct ttttagtcgg tatgtagtac gcttgccatg
     4261 tgcgtatatt ccagcgcggc gtgagtgccg ccatgacgtg ccgaacaaat gccgtttcta
     4321 atttgcgccc gagtatgcgg caactgagta aaaaagaatc cacctcggcc gtatcgccgt
     4381 tacgtgtcac gatgataacc gccgtcaaac cgaaatcacc gaatttatcg ccggcacgca
     4441 tagtccatac cgaagcatcc gggttttctg caaaagctgt gatttgtgct tcggaataac
     4501 gccgtgtggt aacattgaat tgattggttt tttgcgtcag ttgtgccacg cgcggtatct
     4561 catcggttgg ggcttcgtgg atagtggcat acaactgaag ggaggctaaa taatcatcaa
     4621 tagatgtata gcgtgtttct tcgacgcgcc gcatggattc ttcgtgcatc atgcgtgtgc
     4681 gatttttgtc ttctgagctt acctgcaacg catcaaaaaa accggatcgt atcacaaacg
     4741 gcggataggt gtacaatttt tgaggaacct gaagtacggt gacatccggt atcattgttc
     4801 ggactagctc gcattcggcg gggctgtcat ctacaaaaac aaaactgtca aggccgatgt
     4861 tcagctcctc tgcgagggag cgaatattcg ttgctttatc agtccagttg atacgccaag
     4921 ccgcaatatg ttcgcgccga agcgccatat ccggatgatg atcaaaaact tcccacacgt
     4981 cggcctcgtt gtttttactg cagagcgtaa gaagaatacc acgctcatac aaagcgagtg
     5041 cattaagctg gaagtcgcga tacacacgtc ctggaaagct gtcgcggtcc agtttaatac
     5101 tcgacacgcc gtcttcgccg ataatcccac cccaaagggt gttgtcgcaa tcaagaacga
     5161 ggcatttttt ggttttacct tttaatccgc ggctgagttt ggcgatatcg aaggcatacg
     5221 cagacataaa atctttttta aaaggcgcct tggacgtgta ccaaaaacga taatccatag
     5281 ccgcttcttc acccagacga ataagatagc gctcccagtc catcagatag agtcgcgtcg
     5341 gatcgcgacg cacgcgttca cggatctttc ggtttattcg ttgtacttct tcaaaacgtt
     5401 ccgcggcgtc cggtgatcgg tacatgcccc attcggaatc ccaaggcggc acgaatgtgt
     5461 taacggcaag caaggcttgt gtgttttgta aagccgtatc cagcaacgat tcgatttccg
     5521 atatgacgcg gtctgcagtc catccgaatg tacgcaggtt ggtatccaaa gtctcaagta
     5581 cgagaccgag cacgacgatg tccggatggc gctgatacaa cgaggaattt ttatctaaca
     5641 attcctgccg gacattgtca tagttcccga aactgatttc aggacgtatg ccggattggt
     5701 agtgataaag cttgagaaaa ggttcaatgc cttcgatcgt aaaattacgc aaaatatgaa
     5761 tgcgtacgga ttgctccggt tttttttcta aatggtcgag atgatttacc agagcaatgg
     5821 tttccgaaag cgtatcggga agcgtgatgt ctgtaaggcg ttcggggatc atcataacat
     5881 cgtttgaccg ccattgacgc ggatcgtctc gccggtgata taatctgccg catcggacgc
     5941 cagataagcg atagtgccgg cgacgtcttt ggattgggca agccgtttca tcggtgcttt
     6001 catggcattc aatagtttta ctttttcagg tacgtcggct accagttccg tatcggtcat
     6061 gccgggcgaa acgagattta ctgtaatatt ttttgacgcc atttctaccg ccaacgcttt
     6121 ggcaaatccg ttgagcgcat atttggcagt aacataaccc atgagttcag ttggcggtgt
     6181 actttctgtg tacatcgtag ttagcaaaat gatcttgccg tacttttgct tttccatcag
     6241 cgggatgata cctttgcaga ggtaaaatga tgcccgcaca ttgatgtccc actgttggga
     6301 gatatcatcc catgcgaccg aagcccactt gatggccggt attttcaccg tagccgcatt
     6361 gaccaaaaca gttacggtcc cgagttttcg tgttacatct tcggccaaat ggtgaatatc
     6421 ttccggtttg aaaatatctg ctttaaacgc atgtgctgcg cgaccttcgt ttttaacaga
     6481 aaccacaaga tcgaaagctc ttttttcgtt ggaaaaataa tgtacagcga catcaaaacc
     6541 ttgttttgca agcatacgac atgtggcttg accgatacct ccggtagccc cgatgatcag
     6601 cgccactttc tttctcacgg ttgtttcttc agctacggaa ggtttttcct gagcgatcac
     6661 tttgactttg gctttcccgg tggtgacttt ttgtttgtgc tgattgatga tatccgtttg
     6721 caattcgatg atttgttcgg catctatttt acgcaggact tcagcgcgaa cggtgatctc
     6781 gtctccgata cggaccggca gtagaaattc caaactttga gaaaaccaca atgcaccgtc
     6841 acccggaagt ttggtgccga ttactgttga aataaaggat gctccgagca tgccgtgtac
     6901 gaccggtttt ttaaatgagg tgcggccggc gaagttttca tccacatgca ttttgttatc
     6961 atcgccggtc agagcgacaa atttttcaat gtcgcttgcc gtgatcgtgt ggcgaatttc
     7021 agcggtgtcg ccgatttgta tctgtgtata gtcgggcatg tttttttagg gtaagttaat
     7081 caaaataaat cagagcgcac gttttttatt agtaccagcg cagaactgtc gcgccgaaaa
     7141 taaatccggc accgacgccg gaaatcacga ccaattgatc gcgttgcaat ttgccggcaa
     7201 gtacggcatc gcaaagcgcg atagccatcg aggcttcgcc ggtattgcct atggtttcca
     7261 cattggtctg cgttttatgc atgggatgct tcatcttggc catgagaaat tcgattaacc
     7321 gaagattggc ctgatgaaaa ataaaaaaat cgacggcatc ggtttttttg gaaatttttt
     7381 caagcacacg catgatgacg cggggttgaa attgcaccac ctgtttccat acttccaggc
     7441 cgtttaattc aaaatacgga agtacatcgt ttacattctc cgttcgcagt gggtagcttg
     7501 atccgccgcc gcgaacgcgc acggcatcgt acactttgcc gttggccata atatccgagg
     7561 cgagtacacc atatccttcc ggtacacgcg caagtattgc tgcaccggcg ccgtcgctaa
     7621 aataaatagc cgaattgggg tctttccagt tgataaaacg ggattgcagc gcagtaccga
     7681 tcacgagagc ataggctatc gaagaatcca gggtcatgcg atcggccgca ttggccaagc
     7741 cgatttggaa gccggtgcaa ttggccataa catcgtaagc gcccgcattt ttagcgccga
     7801 gttgctcttg cacgcggcat gccatcgccg gacacagata atctccggtg aaggtcgcac
     7861 aaataatcag gccgatttga tcggcagtga tacccgccat aaccatggct tgtcgggccg
     7921 cttcgacgga catgcccgat gcggtttcgt gcgcttcgac gacaaatcgt ttggtaacgc
     7981 cgattttaga aacaatatcg gcggccgata ctccggtcca cgcttcgacc tgctcattgg
     8041 tgatttcttt ggaaggaata tattttccta cgccggtaat gcctataccc atgaatattt
     8101 tatcgtgtta cgttaagcaa atgtatcaaa atgatcatgt acgccgggtt cattttttgc
     8161 cgatgcccac cattcgcgcg cttcaccgat cgtccatggt tttccgtggc ctgcaaaaat
     8221 acgtgccgta tcgtgatgtt cggacagcag tttatctacg gactgtttca gcatagtcat
     8281 gtcgctgccc ggtaaatccg ctctcccgac atgtttgtgc atcagtgtat cgcccgtaaa
     8341 aagggcattg cccagtacga acgccgcgcc gcctttggtg tggcccggca catgcagtgc
     8401 gcgtagggcg agagcaccca gttcaaatac gtgcggcgtc tcaaaaaaat tgaccggctg
     8461 cggcatcgga atgggttgac ggccgaaacg aatcgcatac atcggtgcat gccgtattaa
     8521 tttatggtca tctttatgca aataggccgg aatggaaaac atacgacaaa tcggagttac
     8581 agcgcccaca tgatcatgat gtgcatgggt cagcaaaacc caagtcagtc cgcggtcccc
     8641 gttttgggaa atcagatccg ctaaacgatc ggcatcataa ccgggatcaa tcaacgcttg
     8701 ttcgccggat gcacttgtga taagataaca attttctttc cacagcggat ccgtcacgac
     8761 ttccgtgatt ttaaaaccgt ctaatatata ttcgtaaatg ccttccatgc cgtattcgcg
     8821 ctttcgtcca aggtcagtcc acaagaccca tataagtgac ttggcctgtg atgcaggcgc
     8881 tttcgggcgc ggcataaaat gagatcacat tggcgatttc gtctgtggtg atagggcgct
     8941 gtatggtctg ctgggctaac atattttttt cccattccgg gccaaacgat ttagccgcat
     9001 ccgtcggtga catcgaaggc gcgacgacgt tgcaagtgat tccgagcggc gccatttcac
     9061 gggcaagcac tttggtcatt tgttcgatag cgcttttggt ggccgaatat accgacgtgc
     9121 cgggcgcatg aaggcgcgtc atgatagagg aaatcgtaat gatacgacct tgtttctgta
     9181 gcaacatggt cttggctgtc tcacggcata cgtagtacgt gccggccaca ttggtcttga
     9241 gataggcttc gagtacatcg ccgggcgtga ccgtcatctg gagcgccgat tcgaccaaac
     9301 cggcattgca aaccaaaata tcaatacgac cggcttcttt ttttaccgtg cgaatccata
     9361 accgcacggc ggattcatta ctcacatcca aagccgtgtg atgataagcg gcatgtgtga
     9421 tcgtcgcctc gcttcggctg catccgaaaa cgcggtatcc tttatttaga aaatactctg
     9481 ctaatccttt gccgatacct ttgctggtac cggtgatgat cataaccggc gtagccatga
     9541 tgttatcctt tttgaatcaa gccgacgatg tagccggcta tggtttcggc tgacagaaac
     9601 gggttattaa tattaaaacc ttcgatttca tctaacacaa gaaagatgtc tttgccggaa
     9661 gccgtcgcga tgcgttcttc caaatcggta aacaatgtaa caaaagccat cgaatccagc
     9721 ggggatccat tgccgagaat tacaagatcc ggtgttacct gcacttgcga agcgatcaat
     9781 ccgctgcgcg taagactgtc aaacgattcc tggataacgt ttaaaatctg ttcggtattc
     9841 attcacactc caatattgct aaactgaaaa acggaaacct atcatacgta ttttgagcgg
     9901 ctttttcaat gaacgattat agtcattgaa gatgcctagt gatataatca cgataaggaa
     9961 tgatggttgc cccgtcggca aggatggtat ttattttatc aagcatcgtg ccacggtttt
    10021 gatacatcac ccagttataa aaccggttgt cttttaagcc cggatgcata aagcgctcct
    10081 gctcggtgtc aatatcataa ggatgaaaat agctgagcac cgggcgcgat tgattccagt
    10141 gatgacgaaa ggcgcgcgcc gtcagccaca aaggcaatgt gcgaaagtac aaaccacccg
    10201 cacatgggac ggaaataaaa cgcgaaggaa aaagagtgat tggtaattcc cagatgcgat
    10261 catgcactcg acgaaaggtc gctccaaatt ccggccaacc gtacaagggg ttggtcgctg
    10321 gcagcactga actggaatac gtaaaacctt gctcgatgag aatttcataa gcccatggtg
    10381 tttttttggt caacgaaaaa ataggcgcac gaaatccgtt cagagtctcc acgcccgctt
    10441 tgtaaagtcc gtccatgttg cgaagcaggt catcgcgaaa tgtttccggt gtttgttggt
    10501 caagaggtat gtgccgatgc gtatggcaag caatttcatg ccctgcggaa tagatttccc
    10561 ggattaggga cgggtaggct tctgcgacat cgcccacgac gaaaaaagtg cacgtcatgc
    10621 gatgttgggc caaaaatttc agataacgct ccgtcatggc cgggacgcgt tcacggtact
    10681 ttttgccgtc gctcatccaa aagcgaacgt cttcgagatc aatactaaaa agaaaatatt
    10741 tttccaaaat ggcttcgcag tttttttaat aaatcggcct aagataggag aattggttta
    10801 gcgaatcaac cggagattac ggtactctgc ttatagagga tgtttataaa aaaaattaaa
    10861 tattgttgtc attcgatagt ctctggtatt aaattgtaac cacatttctt aaacacatcc
    10921 tatgctgcct ttttttatca gacataccct cttttggtgg gttttcgtaa gccatctttt
    10981 tgcgcagcaa tattatataa aaaactattc cacgtccgac gggttaccaa gtaatactat
    11041 ttttgaagtt tttgttgatt gcgattacgc gatgtggatt ggcaccgtgg acgggttgag
    11101 ttattacgat ggacaaacct ttcagaattt taataaaact cagggattga tcaatcaccg
    11161 tgtatggtct gtcgctcaag actcatccgg caacatttgg gttggcacac gtacaggcat
    11221 caatcgtttc cacaagaccc gtagcggatg gcaaatacgc gcatggttaa cgcagttgtc
    11281 tgttcgtcac attcatatcg aaaccgatca atccatgata ttgagtacgg aaaacggagc
    11341 atacaagttt acatgtttca ccgatgattc gagttcattg gtgccgattg agggtttaca
    11401 aggaaaaatg gtttatcaaa cattgcgtga tccggataac agattgtgga tagcaacgga
    11461 tcaaggcgtg tggatacaaa aaggaacggt attagaaaat atctcgaccc cttttccgat
    11521 tgtgcggggt ttgttcttac gtgattcatc acacttgtat gctttacatt ttaggggtgg
    11581 tataagtttg acggatcctc gatcggattc cccttcgttt gtttcacaag atttaccaaa
    11641 aggatttacg aaacgcagtt tttaccgcat gatcgttgat cccacaggta atttttgggc
    11701 ggctaccgat ttgggtataa cacggcttcg gttttcagat acacgcgtcg tttcgatgga
    11761 agttattgac gaaaccaaag ggctagccaa taatctgact ataccgttag ttagcgatca
    11821 tgaggggcat atttgggtag gcactcacaa tggtctttcg cgttttgcgg gtttacgttt
    11881 ttcatatttc attcctcata ataatcccgg aattcaagtc tataaatcca ttgccgcatc
    11941 agggacggat atttatatgc tgaccagcgc cggtcttttc tatgtcgatg cgcaacaccc
    12001 cgcagtgcca ttaccgttgt atggtaatac accgatccaa gagggcgtgt cgcttggaaa
    12061 aaacagattg gccttggtca atgacagcga aatagcaatt ttttttgttg gcggaaaaac
    12121 tttcagaaaa caaaaaaaaa tatcggttga aggtcaaagt attgcatcgt ttttgtttct
    12181 cgacgaacaa gatgtaatga tcggcacatc cgccgggctt tatcgtcatc ggcatggccg
    12241 agtaacacgt gtcaatgctt cgacattcac agatcaaatg atggtttata ctcttatgcg
    12301 cgatgataaa ggggcgatat gggttggtac atcacatggc gcctatgtgt tgacagcgga
    12361 tcaggattca ctacgagtcc aatcatggtc tttacccggt gttatgccat ttcaagctgt
    12421 tggtcagatt taccgtgatg cgtcgggcat gatgtggttt tgtaccgaag gcgcggggct
    12481 tgcacgctgg gatgggcaaa atgtacgtgt ttatactacc gataacggta tctcaaataa
    12541 tattgtgtac tctgttttcg aaccggatac gacgactctc tgggtttgta ccgccggcgg
    12601 tattgatgtt atgaaccgaa aaaatgattc atggatgcat tgggacagcc attctggatt
    12661 ttttcggaaa gggagtacgc catactcgat catgaaaagt tccgattccg tgtattggat
    12721 cagcacgagt cgtgggttgg tgcgtacaga tttcagcgaa ccgtttgcgt ccggaaactc
    12781 tgttccggtg ctggtggatc agattcttgt ccaaggtaaa ccccaacgac acagtgggat
    12841 tttgcgtatc gagccgggag ttcagcaaat ccagtttcgc atcaaggcga tatcttaccg
    12901 ttacgagaat gatgtgcggt acagtttttt tatggagggt tttgacgaag cttggtcgga
    12961 gccttcctcg caaggcgtga tacaatatgc gggcttatcg ccggggatat ataggttgaa
    13021 aagcaaagcc atcacacccg gcggacaata ccgcggaccg gatgtggttt tggaagtatt
    13081 gaccccgttt tggaaagaga cgtggtttgt agcgatatcg tgtttggtgc ttatcgccgt
    13141 gacggccggc gcctaccggt ggcgaaccta tcatttatgg aaaaaagcgc gccagctgga
    13201 aatgctggtg cgcgatcgca ccaaagattt ggtgcgtaaa agccgagacg tgatcaaaca
    13261 aaaaaaagaa cttgaaaaaa cattacaaac cctacgcact accgaagcgc agctcatcca
    13321 agcggaaaaa ctgtctgctc tgggtgaact cacagcgggt gtggcgcatg aaattaataa
    13381 tccgattgcc tttatttacg gtaatttacc gttgctggaa aaagatcttc gccgtttaac
    13441 ggagctggcg caaaaagcta aaaccggacc actggacgac aaagaggcgc tggaaatgac
    13501 tcaaattgaa aatgatattc attcggcgat tgtatcaacg cggcacggag tacagcgggt
    13561 acaggttatc gtcaaaacgt taaagaagtt tgttcgactg gatgaagcga aagaggattt
    13621 taaatccgtg gaagaatgtc tggatagtgc ggccgatgct ataattatcc agtatccaaa
    13681 ggttcagatc aaaaaggatt atcgggagac gccacaaatt ttgtgttatc cgtccgaatt
    13741 gcagcaggcg ttttatcatg tcatgctcaa tgcgatacaa tcgcaagcaa ccgatgcacg
    13801 ggtggacttt tcaacaagca ccgacgggaa atatgtgtat gtgggaattc gcgactatgg
    13861 cacgggcatc cctgaggcta taagccaaaa aatattcgat ccttttttta caaccaaaga
    13921 cgtagggcgc ggaaaaggat tgggattgtc tatgacttac gggatcatgg aaaaacacga
    13981 gggtgggata cggtttgaaa actgtcaaga tcagggtgcg aaatttatac tgatgttgcc
    14041 tgtagagcgt aggtcttcat cgccgtcggt gttatcgtag tcgcatagaa gaattgcagg
    14101 aaagaaataa atagtactta agaggttcga taaaaaacga atgttcttat atcacatgaa
    14161 tcgggaataa tagactaaat gtactaccca tgccttcatg gctttttact accaaacgcc
    14221 caccgttgat ttctgtaaga tcacgcgcaa tggcaagacc gagaccggca ccttgttgtt
    14281 ccatttcagc gcgattgact tgatagaatt tgtgaaaaat acgatctaac tgctcctccg
    14341 gaatgcctat gcccgtgtct tcgatatcca aacgtacata gtcgtggtct ttgccgctgc
    14401 gaatacgaat ttctccgccg ggcggagtga atttataggc attgctaaat aagcgctcca
    14461 ctacatcggc cacttgatct acatggagtt gtaaccccgg caaatgccgt tctagctgag
    14521 attggattct tagttgtttg atctccgccc gctttttgaa ctgcggtacg agatgctcaa
    14581 cgatgaagtt gatatcagaa tcttgtttgg cttctttgta ttcagatttt gcatcaccgg
    14641 ctgcaatgcg tacggtgatc aggaaatctt tgagcagggc atctagacgt tcgccgcttt
    14701 ttttgatgcg aagaagaagt tcttgcagct catggggttt aaactccatg gtttcatcaa
    14761 gcaaaagatt ggccgtaagc ttgatgataa aaagcggagt attgacttcg tgtgtgatcg
    14821 tcgaaaggat ttctcgtttc aattgatcga tttgcgtcag catttccgct tcggtgctgg
    14881 agcgaatctc ttctacgcgt ttgagtttgc cgcgaataga ggcaaccaga tcttcttgtt
    14941 cgatcggttt ggttaaaaaa tcatccgaac cgatttcttt accatagcga cgtttgtcgg
    15001 cgtcggaatg tgccgtgaga aaaataaatg gaatgctgcg aagatcgggt atggctttta
    15061 cgttgttata taattcgaaa ccgtccatgt acggcatcga aatatcgctg atgatgagat
    15121 cgggcttata cgtttttagt ttttccatcg cggcaaaacc attgggcgca agtgtcacgt
    15181 caaaattcat ccggctcaga taaatctgaa ttaattgcct gttttggaag tcgtcttcga
    15241 cgaccaatat tttggcgtga gtgctcatgg atttatttga cgcgcagtcc gccgggcatg
    15301 acggcctccg gggataatat tttgagtgtg gtaccgtcgc tgaccgtcag gatcatcccg
    15361 ttggattcaa cgccgcgtat tttcgccggt ttgagattgg cgacgatgat cacgtctttt
    15421 ccaatcaatt cttccggctt ataatactcc gccaaaccgc tcacaacctg acgtttttca
    15481 aaaccaagat cgagctggag tttgagtaat ttggttgtgc cttcgatttt ttcggcatgc
    15541 agcaccttgg cggaacgcat atcgatttta ccgaattcat caatacttat ttgcgctgca
    15601 gtgaccgctt cattttcgga tttcggtttt tcaaccggtt tggactgcgc actttctgtc
    15661 gttttttgtt cgatcattga gttcaaatga tctatctcct tttgtatcac atcatcttcg
    15721 attttaggga aaacaatttc aactttgccg agcacgtggc cggtgtccat tcgcgaggtg
    15781 ccgatggtat tccaatcctg ttctttgact aggcccgaca aaccgagcat ttgccatact
    15841 ttttcagagg taaatggcaa gaaaggatgc atgatcacgg ctaatgcgcg tacgacttgt
    15901 acgctcaccc acaaaatcgt agcgccgcgt tccggttcgg ttttgatttt tttccagggc
    15961 tccgtatcgt taaagtattt attggcggcg cgtgccaaat ccataaaacg ttttaacgca
    16021 tcgcggaaac gaaattcttc aataaatttg ccgacttcgg tttgcgcggc caggatttcg
    16081 gaaagcattt gttcatccat agccgtaagc gtgccgcgcg acgggacttt gttgtggtag
    16141 tttcgaatcg taaaggacaa actgcgattg atgaagtttc cgagaatatc tgctagttcg
    16201 ctgttatttt taatctgaaa atctttccat gaaaaatcgg aatctttgtt ttccggaaga
    16261 ttggatgtca acgtgtaacg caaaagatcc ggaggaaact tttgaagata atcttttacc
    16321 caaaccgcat aattacgact ggtcgaaagc ttgttgcctt ccagattgag aaattcgcac
    16381 gccggtacat cggaaactaa aaccaaacgg cccgatgtct tactttgatt gtaggccatc
    16441 gtgatgatag ggaacatgat cgcatgaaat acgatattat ctttgccgat gaagtgcacg
    16501 agttgggtat cgtccttttg ccaataatca cgccattttt caggttggcc tacttttttt
    16561 gcccattctt tggtcgaaga aatataaccc aatacagctt caaaccagac gtagattact
    16621 tttctatcgg aaccttcgat ggcattattc ttacggtctt ttttgggaac cggtacaccc
    16681 caatcaatgt cgcgtgtaat ggcacggtcg ccaagtttct gtgcaaacca tcctttacag
    16741 tagttgatga cattgtcttt ccagtgagac ttggtagata tccatttttc cagttcggat
    16801 tgaaattcgc ccatcggcaa ataaaaatgg gtggtctctt tggcgatcgg agtagtgccg
    16861 gtgactttgg agcgcggttg gatcagatcc atttgattca aatacgtacc acatttttca
    16921 cattgatccc cgcgagcatc cgtgttttga catacagggc aggttccttc gacataccga
    16981 tccgagagga acattttggc ttgttcatca taccactgca tctcgggttt gcgtttgagc
    17041 agattttttt caagcaagtc gagaaaaatt tcctgcgccg tatcgtgatg aaccggattg
    17101 gatgtgcggt cgtaatggtc gaagctgatg cccatacgag aaaactcgtc ctgaatcatc
    17161 gcatgatagc gatctacaag ggcttgaggc gtaacccctt ctttttccgc ttgtatcgtg
    17221 atgggcacgc cgtgttcgtc gctcccgcaa atgtaaacga tatcgcgacg catgaggcgt
    17281 tggtagcgaa catatatatc agccgggaga tatgcgccgg taaggcgtcc gatgtgactg
    17341 ggaccattgg cataaggtaa cgcactcgtc acaagaatgc gtttaaacga tgtgtcggta
    17401 ggcaaagcta actccgtagg aaaaacaaac tgtctgaatc taaaaaatta tttggttacg
    17461 taaacgactt cttgttgtgc gcaggtgagg cgcaagtcgc tgtatttttc tttgaggcgc
    17521 agttcgatac gatccagttc acggtcggtg cgcatcggtt cgtgatgcgt gagtaataaa
    17581 tgtttacact cgatctgctg cgcattgcgg caagccatat caaatgtact gtgcccccaa
    17641 ccgacacgtt gcgcgtattc ttcatccagg tacatggcgt cgtgaatgag catatccgcg
    17701 cctttggccc aggtaataaa ttcacgattg acagaattat caatagcttc gtgatcgctg
    17761 gcgtacacta cactgtggcc gccgtcgctg attttgtatg cgaagcaggg gtcggtgtgg
    17821 ttgagcgcac ggcatgaaat ctgaaatccg ttgatctcga tatcttgcgt aagctggtga
    17881 aattcgagct tggaattgag ttgctgccat ccgttgaaag ggccgtagga accgtcaaag
    17941 tagatatcca tcacttcttt gagtttgtca ttcgattgtg gcgaataaaa atgattgata
    18001 cttttggggt ggtgcatagc gccgaaatag ttgagaccct gaatgtgatc ccagtgaaag
    18061 tgggaaatca aaaggtgaaa ttcacccatc ttgtttttaa gatgatcgga ataaaatcct
    18121 ataccaaaac ccgcatcaat aataacactg cgctctccgc tggaaataac cacgcatgcc
    18181 gtatggcttc cgtatttgag cgaatcttcg tgtgtcgcgc tgatcgagcc gcgcgtaccg
    18241 taaaacgtga cttctaaacc catacttccg ttccctcccg caaggccacc acttcgatag
    18301 cgcctgcttt tccgatggat tttacatagt cattggccga agtgattttt tgctctacaa
    18361 aagtgtccgt atcttccggg gcatggtgcc cgagtatgat ctttttcacg ccggctttga
    18421 ctgcgagatc gacagccatt tcgggtgtcg aatgccccca gttttcttta cctttaattc
    18481 cttccttggt aaagtgcgtg tcgtgcagca tgatatcggc tccggtgcaa aagcgaacca
    18541 tattttcgta gaattctttt tctgttttag tgaagtcttc tttgttccat gctttcatca
    18601 aaacgccgag atgattgttt tcgattggcg catgatcggt gatgatcaaa accgattttc
    18661 ctccgtgttc gatccgatag ccgagcgtta atccctggtg attaagttgc acggttttga
    18721 cggtggcgtt ttcaagcggg atcgtctgat cgggatgtaa ttcgtggaaa gtgatcttat
    18781 cgcgtaccag tgaaaaagga atcggtatca aacctttggc atgctgaagt ttgaaaagtt
    18841 cttcgatggt gtattcttca gagcgcgggc cggatatgga aattttggtg tttttattaa
    18901 ataacggtgc gaaaagcggg aatgcgagaa tgtgatccca gtgcgtgtgc gtgataaaaa
    18961 agtgtgcatg aaatccttca gggtgctgtt cggaaatcgt aaccccttca cgatagatgc
    19021 ctgtgccgcc gtcaatatag attggctgca taccgtcaat cgtgattttg aaggctgttg
    19081 tgtttccgcc gtagcgaacc cggtccggcg tcaatatcgg acgcgaaccg cgtgttccaa
    19141 gcagtgtaaa cttcatgccc accgtgtact cccggctttg ttgattattt ataatcaacc
    19201 acgaatacta cacgtttgtt acgcaaacgg taatattcgt ggtgttatgt atctcatttc
    19261 aaaaaaaacg atattatgct ttcggaccag cttttttaac gtcgtccgaa gctttatcgg
    19321 caaatttttc aaaatttttg atgaatagat cggccaattt tttggcttgg gcatcgtaac
    19381 cggctttatc tttccacgtg tttttaggaa cgaggatttc agccggtaca tcagggcatg
    19441 attccggtac agccacgccg aaaaccggat cgttattgta tttcacgtta ttgagtttgc
    19501 cggacagtgc agcattgatc aatgcgcgag tgtaactcag cttcatacgt gagccgacac
    19561 cgtatccgcc accgctccag ccggtattga ctaaccatac ggtagcgtta tgttcgttga
    19621 gttttttacg caggagttcg gcatatacat tgggatgcag cggccagaaa ggggcgccga
    19681 agcaggttga gaaggttggc gtcggttctg tcacaccggc ttccgttccg gcgactttag
    19741 ccgtataacc cgataagaag tgatacatcg cttgggccgg tgtcagtttg ctgatcggag
    19801 gaagtacgcc atatgcatcg caaacgagga aacaaatatt tttaggatgt ccgccgatac
    19861 ccggaatgat gcagttatca atgtattcga ccgggtaagt gacgcgcgtg ttttcagcga
    19921 tcgcggcgct gtcatagtcc ggcgtaccgt cttgtttgag aacgacattt tccagtacgg
    19981 aaccaaaacg aatagcattc cagatttgag gttcgccttc tttggagagt ttgatggtct
    20041 tggcataaca tccgccttca aaattgaaga cgccttcgtt ggaccatccg tgctcatcat
    20101 caccgataag acggcggagc ggatcagctg agagtgtggt tttgccggta ccggacaaac
    20161 cgaagaacaa agtcacttcg ccttctttgt tgctgttggc cgagcagtgc atggggaaaa
    20221 taccgcgctc gggcagaata taattcatta ccgtgaaaat cgattttttg atttcgccgg
    20281 cataatgtgt accgccgatg aggacgattt tcttttcaaa attgagcatg atgaaagtct
    20341 tcgtgccgag accgtcaaat tccggtatgg cgtgcatacc gcaaacatct atcactgtcc
    20401 aatcggcatg atgcgtggcc aattctgctt ccgtagggcg aatgaaaaga gttttagcga
    20461 aaagcgaatg ccatgctttt tccgatatca cgcgcacgga caaacgattt ttagtgtcgg
    20521 cgccggcaaa gccgtcgaac acaaatacgt ctttaccttg cagataagag cgcatgcggt
    20581 taaacatcac atcaaactga gcttgcgaaa cttttttgtt gttttcccac cagattttgt
    20641 ctttgctggt cggctcttcg acgacgtatt tgtctttggg ggagcggcct gtgtgcggtg
    20701 tcgtattggt cacaagtgca ccgttggatg caagctgacc ttcgccgcgt ttgattgcaa
    20761 gttcgactaa ttgactgacc gaaagatttt tgtggattgt accggtatta acaataccaa
    20821 ggtgagctaa ttcattgtta gtagtcattg gaatatccat ctcctaagtg attatcgagt
    20881 tccgctgtca gattttcgga cgcggaaagt tgtattggtc aaaaaaagga tcattttcca
    20941 taaaatcttt gagcggattt tgcatcgtgc cttcttccac gtgatcgtgg gtattgcatt
    21001 taccgctttg gcaaccgtcg ccgcccgaag cgcaaccgct gccgcaacca tgtatatcgc
    21061 tcatgtcaat gccgagaatg tcggcgacat cctccagcgt gagcgtttcc cattcattat
    21121 catggtcttg tttgatgtgg actttgttgc cgatgatatc aagatgcttg atctcaccgc
    21181 ggcctttttt ggttttgatc ggagaaccga cacccggaaa tcgggataac gtattaacat
    21241 aaaaatcacg ttcgtatgcc aagcaacact tgagccgccc gcacaatcct gtcagcttgg
    21301 aaggattgag ttgtaagccc tgatctttgg cgtattccgt ggtgatcggc tggaattcgg
    21361 tcaaatgccc cgaacagcaa agctgtttac cgcacggacc gtaaccgtca acgcgtttgg
    21421 cttcatcgcg tacgccgatc tgccgcaact cgatgcgcgt tttaaattcg ccggccaaat
    21481 ctttgactaa agaacggaaa tccacgcgct tttcagcggt gaaaaagaac acgatttttt
    21541 tacgatcaaa ctggtattcc gcatccacca gtttcatttt aaggccgtgt tttatgattt
    21601 tttgctttac gaccgctttg gttttttctt cttcgttgcg gttatcggcc aatttgctga
    21661 tttcatccgg attagcaacc cggatgaatt ggcgcaattc tttgtctttg gtgagatctt
    21721 caggtatttg ttcgtgagtc agagcgacct tacccatatc aacgccgttt tcgcattgca
    21781 cgataacgtg gtcgccgact ttgacgacca gattgttctt attgagaaag atctccttgc
    21841 ggttggcttt aaactcaacc tgaataggat tttgcataaa atatataaat caccgtttta
    21901 ccggcgacta tttcccatat tgttaggatt taccggatga agggttgacg atggaacgaa
    21961 gattcaaccc taaattcatt aaaattaggt taatatagat atttcgccct ataaggtcaa
    22021 ccgctttttc gatttcagac agagcttctt cgacacgaac gcggggaaaa gctttgatga
    22081 atttttcaag tcgttcggtt ttatcttcat tgatcaaaaa gcgatctatg agcgacttgt
    22141 cttcacccaa ttcgcgcata tacacgacat cctgaaacca agttatcaac atcgtcaaaa
    22201 tgtccgtata aaaactctta tccttttcgg cctcttcgtt ccactgttcg atgatcgtct
    22261 ggatggatgc tgatttatta gacatcgctg ccaataagaa ctccagcatg gcttcacgct
    22321 ttcgcggaaa gtctccggag atcagttcac tggcctgata gtagttaccg ttggccagtc
    22381 ggctaatgaa tttagcctgg ttttcagcta catcgtagtg acgacggata ccgtctaaca
    22441 gttgatcatc cgtgaggcgg tgcagtttga caatctgaca gcgcgatatg atcgtcggta
    22501 gcaggctgtg aatattggtc gtggtgagaa agtataacgt ggatgccggc ggttcctcaa
    22561 ggatttttag aacggcattg gttgcttccg cgtgcatatc ttcgataaat gtcagaataa
    22621 aaattttgag ttcgccttca aacgaacgca aactgatcgc tcgggatagc cagcgaatcg
    22681 aattgacttc cggattttcg gggtcgccga taggaatact gccattggcg atgcgaaccc
    22741 gggcgtacag gtttcgcgtt ttttcttcca ataaacggcg ctgttcatct tcttcggtgg
    22801 ctcgcatcac cggaaaataa tagtgcacat cgggatgctg cagggtcgcc atttttttac
    22861 aattggcgca ttctccgcac ggttcccatc gttcgcgatc cggatgtttg catagtataa
    22921 agcgtgttgc ttccagtgcc aattcttcgg cacccaatcc ttcatcgccg gcaatgagat
    22981 aggcgtgtgc catgcggtcg ttggcaaatg ccgtgagtat ctgtttttta gcggcctcct
    23041 ggccgatgac ttttgaaagc atgagcggaa tctatgtgag cttatcggtt gggctgatta
    23101 aaacagatat gaattcatcg aggaattcat cgtcatgttg attgcaacgc gataagcagg
    23161 tgttacatta ctatatttat caatatatgc gcatcgtgac gccttatcaa gagtatgtca
    23221 taacttgaca aagtctgtcc ggcgcatgtt actttttttc ctccggccgc attttaaaaa
    23281 tcagtgcgtg gccgttttga atatattttt gagcgatttt gcgaatatct tttgccgata
    23341 aacgacggat caatttttcc tgtgcttcgg gagtaatgtt tttgatggaa gcgttttcgt
    23401 aataagcgtt ttcgatccat cgtaaccagt aagcgttctc tttttgattc cgctccagat
    23461 cacggcgcaa tgcttcacgg attttgttta catcggattc ggccggacca ttttctgcta
    23521 atttacgaat ctcctcgtgc actgcaccga tcagggaatc taatcgctcc ggtgcgcacg
    23581 agaaaccgat gatcacggcg taattagata ccggatattt attggcgtac gcattggcgc
    23641 ttgtgccata cactccgcct ttgtcttcgc ggaggttttc gatgagttta ttggtcaaaa
    23701 gcattgccgt agcgcgcaat aaaaaaagat cgtcgtattt tttcaaagca ccgtggaact
    23761 gcatattgac ctgagcacgc ggatctacac cgcgataaat cgtggtatcc atattccgac
    23821 ccggcctgcg aattccgaga tctttgaatg tctctttacg tccggtcgta ggtaaaccgg
    23881 ccaaataacg ttcgacaaaa ggtcgcatcg tctcgatctc aaagctgccg acgatcgtca
    23941 ttacgaaatc gccggcttcg gcaaaacgat cacggtatat cgccaatgcc gtttgcggat
    24001 tgatttctga aaaacgttca cgccgcaaaa ctgagggatc acggggatga tctcggctga
    24061 gaacacgatt gagttcgttg ctaaacacaa agcggggatc gttgtccagg tttttgatgt
    24121 aggccatttg ccggctgagc aaggcgtaaa aaaccgtagt gtccaatcgc ggtgcagtaa
    24181 agttgagata gatcagttgg aacatggttt ccatatcctt tttaagcgac tgcccggata
    24241 tggtttcact caacatgcga atgcccgtcg aaacggatac gtttttgccg gatagaattt
    24301 tttccaaatc ggttttagaa aactcagcga tgcctccttc acccagtggt gccatgccgt
    24361 aaatagcaga catatcctga gagggtgcac caagcgacca tccgccaaag ctgtatgcgc
    24421 tgaatttgat ttcatcggtc atgtaggtgg tcggtttcaa aatcactttc attccattgg
    24481 ataacgtcca ttcttcagcg gcaatttctt tgagtttttt tgttttgacg attttgccgg
    24541 gatggggcag cgttgctaaa agcggtacct gtacggtttt atatacgtac ggtactgttt
    24601 ggatcgttgc tacagagtct attagcgcaa gcaaacgcgc ttcgtcggga agttttacgg
    24661 tctcattttg gggtgcggtg acgacgccga tacgattttc cgtggtaatc cattgggcgg
    24721 ccaaagcatg aatctcatca acggtgatcg tgggaagaag acgtttaacg gtctcgtatt
    24781 cccatgccat gccgggtgcg gcctcggcgg taagcgcata ttctacgaat tcatcggcaa
    24841 gggatgaaga aagtactttg tcgcgattat tgtatgaaga ttcatagttc gaaaggattt
    24901 cttttttata atcttccatt tcatcgcgag aaaaaccgtg ctgttgtgca cgacgatttt
    24961 cgatgagtac ggcgaggagt cctcgttcca gagccgtttc gcccgtaccc gcatacacgg
    25021 catacacatc cgtggcacgc acaaaattac cataaaaagc tccgccaaaa acaaaaggag
    25081 gattggcttg ctgagtcagt tcttccatgc gtttattgag catcccggtg tacagcatac
    25141 gcaataggga cgtacgataa tcctgaactg aacgtacggt ttgggatggg tgtttgtaaa
    25201 aaatctgggc cattgtgttg ggcgcttctt tgtcacgcgc aacggcagtc catgtttctt
    25261 tatgcggagg tacgggaaaa tcattacgcg ggcgcacttt tttggcatcc gacgtagcaa
    25321 tatttgaaaa atgcttgcga atcagatatt cgatggagtc cggttggatg tcgccgaccg
    25381 cgatgacgga catcagttcg gggcggtacc aatctttgta aaaacgtttg atcgaagcgt
    25441 gggaaaattt ttcgagaatt tctttttttc cgatcggcat acgttcggca taacgcgaac
    25501 cgtaaaataa tttgggccaa tattgatcgc tcatgcgttg ctgagcgccg ataccggtac
    25561 gccactcttc gatcacaacg ccacgctcct tttcgatttc gaggctgtca aatgtgatgc
    25621 cgtgcgccca gtcttccaga atgaggaatc cgttgtgaat aatatcctgt ttgtcgctgg
    25681 gtagtgtaag catgtacacg gtttcatcga acgacgtata cgcgttgata tgtgcaccaa
    25741 atttgacgcc ggccgattgc aaatagctga tcaaatcatt tttttgaaaa tgtgtcgtac
    25801 cgttgaaagc catgtgttct acaaaatgcg ccagaccaag ttgatcgtca tcttccatga
    25861 tggagccggt attgaccgcc aaacgcaatt ccacgcgttt ttctgggcgc gcattggttt
    25921 tgatgaaata tcttaacccg ttggacaata cgccgctccg taccgcagga tccataggta
    25981 tcgtttcatc ggtttgagcg aatagcgaag taagactaag cgataaaaaa agcagaacaa
    26041 acgttcttcg ttgcatgatc atgtccttta ttttttacat atctataaga tcaatctttg
    26101 accagtatca catctaattg agagcctgat ttcatttttg atgttatggt tttaccgccg
    26161 ttgatgactt cgcatctaga aaaaataccg tcggattcgg ttttgctgac tttgattttt
    26221 ccgatatcct tggagcgctt tacttttttt ccatccaatt cgacttctgt atattcgacg
    26281 actttaaagg catcgttttt tcgtacaccg gatgcgctgc cgcaagtgat cgcgacggac
    26341 acggcctcgc cttttttctc ttttagaatt tcaaaaattt tccctcgcac tggaaacgat
    26401 gatttgagcc aatctttcag cttgtcgctg ctcatattca gtgcgttcga tagtgcttcg
    26461 gtcgtggtcg tataagcgag aaaccccgtg tcgcctttta ggtttttggt ggtagttact
    26521 tcgcccgtag atacttgcac aaggcggatg ccatattcta gaaggccgct atactgcgta
    26581 ccacgatcac cccaggtttg tttttcttct tcggtctgag tgacgagggt cgtctgtgcc
    26641 tgatagagat gcaaaataac cagaaaatcc gcgcccatca gttcccccaa tcgtaccggt
    26701 gtattttcag cggcgagatc cgacatttgg aaatcctgtt cgctaagaat tttatccatt
    26761 gccgcgcgtt ctacgacggc gattcgtttt aacccaacga gcgtcgagac gattttttga
    26821 gtcagcgcat cggcgatgac gttgacttcc gatgagccgg tcgcggcgct agttacgatt
    26881 ttggtaatat ccggcggcag tacggccaca gtagttttag attccgcagc ttgcgcgaat
    26941 agttgcgaag ctgagaatga aacaaataaa aaaaaagcgg tgattttcat cggttacttc
    27001 cttttataaa atacggtgac tattcggatt ggggtttgtg tatcatggat gaaaataaaa
    27061 tacgttcttc atcatgtaaa aacagtttcc atccgacata aagataaaac gccgtaaaaa
    27121 tacaaacaca tattatatcg taaatcaaaa gatcggtcca cgccggaaaa agaaaatcca
    27181 agcctttaaa aataatataa catccaagac caatccatac gatatgaaga agatcccgac
    27241 gaaaaggacg tattttgagt atgagatgta cctcggcgat ttgtaaagcc gcagttaata
    27301 aacatccggt cagattgccg atggccgcac caaatatccc gtacgtgggt ataagtataa
    27361 aatcaagggt catgctcaca gcaacaagaa atacattgtt aaataaattc aaataaggat
    27421 atcccgacat gacaaggacg ggttcaatgt tgctggtaac ggtatgaatc atcataccga
    27481 agcatacgat agataatgcg ccggcggcat aagcaaattc gtcagcaaat atacgcagta
    27541 aaaaatcacc gtaaatcgtc gtaagaacaa aaaagccaca ggccaaaaaa agacttgagc
    27601 gaatcagcgt agcaaaaata ccctccaggg cgcgcatatc attgcgctgg cgcatggcga
    27661 ccacgaccgg catcatgata gggtcaatcg cttgcggtat attacgcatg gtgttgccgg
    27721 tattgaggat gacctgaaaa acggcgactt gtgcgctcgg cacgagaata ctgagcggta
    27781 tgatttcgac tttaaggatg actactgaaa taaaatcggc aaacatcacc gggaagccga
    27841 aagaaaaaat gcgtttccaa gtcgaaccat ccggaagatg tgaaaaaatc cgccgcatcg
    27901 aaaaatgttt tctaaaataa tatatcgata acccgctacc gagcgtaaat gcgatcattt
    27961 tggcccagaa cggtgcggcg atcgaaccga aaacaaaaaa cacgacgaga agcagaatca
    28021 gatccaccaa cggttcaaat ccattgcgaa taaaaaccca aagtttcatg attttgaggc
    28081 cgcgtgtggc ggtcatgagg atggtggtaa ggcagtaaaa cggtgcacac cataccatga
    28141 ttttcatata gtcggcgacg ggcggtttgc ccatataagc ggctagatga tcggcgaaaa
    28201 gatagacatt gagtactaag aaagcgctga gtcctaaact caggatgata tagccaagga
    28261 tgcttttata atattcgtct tcgtcggtat ctttgtagcg tacgacaaaa tcaatcacca
    28321 gccggtgtgc gccgaaataa cacacgataa atgataagtg catcaaaagc catgtcgtgc
    28381 tgaagtaacc gaaaagagtt tgtccgagca cacgcgtagc gatcagatca aacgcaaaat
    28441 gagatagttt gccgaaattg ccgatgaggt tgataaaagc cccacgggcg acttcgcggg
    28501 tttctttgtg gtgttgcgac gattgggatg tcaaaaacga atgatactct ttagatgaaa
    28561 gaacgccgta tcaaggcgtc ctgaaataca tttgctgcat tcataacaaa aaaaacagac
    28621 aaaaaaaagc cccatctgca taagcggatg gggctcataa tctttcaatt aatgtgattc
    28681 caagatggct agttttctga ggcttcgaca gctatgatcc cgctacctcg caaagcattg
    28741 cttgcatcat aagcttctac tttcataatc aatacgagag gaatagcgcc ggtacccatt
    28801 acgcccacgt aactggttcc tgtactttga tagctgtatg tagttccata aggatatgtt
    28861 gtttgtttaa ccaatttata ccatttgtaa gtcgttgcat ctgggatggt tgtgacagta
    28921 tacgtcgcac taccattcgt tgggagttca acgggtccgt tgatttgcag ttgttttata
    28981 aagtttgcat taagtgatgc gtttgaatta actgtgaatg aaatttgatt ggattgagag
    29041 ttgtttgcgt ctgaccagtt atagaattgc caaccggatg acgggctggc tgtagccgtt
    29101 acaagagttc cttgttcata tttaccatcg gctcctggtg ctggcgataa ggaaatcgtg
    29161 ccgctaccgc ttggatttac aactgttgta atactataac gcggaatcaa ttctaaataa
    29221 atatccaaat gagtatatgt aatactacag agtgctccga cgctaaccaa actaaaacta
    29281 actctattat ttggaatggc attaattaca gcgttattga gtgatgaatt atctaatatt
    29341 acagggaaaa agccggtttt tttggtacct agagttgtcc caactgagca agcgtcgtag
    29401 tgaacttgag tgccatacaa agagtttgat gtaaataatt taatgtcaat gtcaccataa
    29461 atttcagatc tgctaactga gccggaagtc gggatcgaat tgctaagtgc taattcacta
    29521 gacctcactc tgtagcctga aggaatatta ttgatgccgt taaaagtaac aactaatctt
    29581 gaattttcgt tgttataagt aacggattga tcgtcgggca gatacccggg ataactaccc
    29641 aagttatcaa aaataggtgc gtattctgtg gtggtgatat tattggatat tcgtgtatat
    29701 tttaaagttc cagtgattga gccttcaatg attcctgcgc ttttttccgg ttttttattg
    29761 tagtcaatgt ctatccactg gccatttact aattcatgca ctttttgtgc gctgaaacgt
    29821 gttactactt tcccattact taattgaaac gtttttgagt attctgatct aagattctct
    29881 atttctgtgc catcaatacc tgtaaaaaga ttgaaatttt ctccaccact ttttatttct
    29941 tggttgctgt tggttttttc ttcacaacca agcatggcca ttatagccaa actcataaaa
    30001 accaaatgca aacttttgaa catggatttc atgcgctttc ccttttttaa taaattgaga
    30061 tttgttagag aattaaacat tattaactaa taattgatgt tggaacaggc tccttatttt
    30121 ctgaaacata gttaattaaa acgatagttt tgtgctacct atatttagta gtacaaaaaa
    30181 acgtaaaaac atttcaagaa atcttgacga ctatatgagt gtaataaata cacgaccaca
    30241 cacattgctg tatttgcttt gaaaaatgaa tgggaaaaat gggattaaat gcgaaatacg
    30301 atataataca ttccctttat acccctaaaa acgattttcc tgctaacact ttagcataag
    30361 tgagtctaaa cgccaaggca aaaaaaagcc ccatctgcat aagcggatgg ggctggtata
    30421 atatagaaca cgactactta ataagattca ttttcttgga cattttaaag ctaccggctt
    30481 caagtcggta aatatagaca ccgctcggta cttgtttgcc ttgattgttt ttgccatccc
    30541 aaagaacatt tttgaacccg gctgtctgat gctcattgac gaggttacgc acttcctgac
    30601 caagaagatt atataccttc agactgacgc gcacatcttc tttgagtgca tagcgcagcg
    30661 tcgtcgtcgg gttaaaagga ttggggaagt tggattctaa gccaaattcc ataggaattg
    30721 tactcagatc tttttgcatt tcacccgtag tgtccgtgta ggctgattca taaaatgtgc
    30781 cggccagagt ttttgtcatg gtgccagata tatgagggaa agttgtggtt aaccaggatg
    30841 tgtttcccat caaagttcct tggctattag ttccggcaat gtcattgatt agagatccgg
    30901 aaccctcgtc gagtttgtaa taaagaacaa gcccggattc atttccattt aaatgcgtgt
    30961 tcattgaagc ttgtatttca ctttgagtgc gagcaaaatt ccaccagcga acttcatcga
    31021 ggttgccttt gaaatatgcg ctgttgatgc tgctattgcg tccgatattg atgtcgtagt
    31081 tatcggtgta tatgtaaccg gaataagggg aacctgtgcc tacatgttca taaattccat
    31141 caacatatac tcttggagtt gtgagggtgg ttctgtccca aacgatggaa atgtggtgcc
    31201 attgatcaaa tccaacttga tcgtcgacgt tgtagaaaaa ccatcccgca ggattggtac
    31261 ccctgaaatc aacacgtagt gaggttgaag cgggattagg attaggcgtt gggtcatagt
    31321 aaaaatgaat gttgacgccg ttgtttcccc atccgccacc tttactgata atatgttgac
    31381 tattgccgcc cgattttctc agtacccatg cttcgatggt aaacgatgat atagtgttta
    31441 aggatgattc ttgaccggcg ttgacataat cgcccgcatc attaaatgct aaagcaaact
    31501 tattttgtgc catattaatg gtgcccttat ccaaaacggc attactcgga atcgtaatcg
    31561 gaatattttg tgtttgataa tttgcatcat tgtcaaaagc cgtcatcgta tatgaaccgg
    31621 gtgttagttg gatatacacg tcgccattgg cattggtggt ttgtgtggct actgtattat
    31681 tagaaccgtc tgttattttg actgtttttc cgctaatcac tgcggtattg ttggaattga
    31741 caaaatgcgc tttgacaccg agaggggtaa gaacacgcgg tgtatcaaca aaggcagcat
    31801 ttatttcgcc ggcggttact gtagttgttc cggatgtata gcccacataa tttccagata
    31861 aggttttaac cttaaaatca cctggtggca gtaattgtgt tattatacca ttggcgtcag
    31921 gataaagtgt tccagaatag tttccggcgg aataaacata agtaaaagtg gcgtcggtta
    31981 ccggcgtacc atcagtgctt ttcacaaatt tagcacgtag tgtggcaagt ggagtcaaag
    32041 atgtggggtt ggttatgagt gtagaattag tttcgccggc gactaaagta accgtacttg
    32101 agttggcgct gacgtaattt cctgtaatcg gttttacgta ataatttccg ggagctaata
    32161 cccactccat ttcaccttga gcgtttggtg tgcgcacacc atagttctga ttatttgaag
    32221 ccaatacggc cgtaaatgta gcatctgtta caggcgatcc atcggtgctt tttacgaatt
    32281 tggcgcgtaa gacggtactc ggagtcaaag aagtggggtt ggttacgagc gtagaattag
    32341 tttctccggc aattaaagta actgtaacag aattagtagc aacgtagtat cctgttagga
    32401 gctttacata ataatttcct ggaggtaatg cccactccat ttcaccttga gcatctggtg
    32461 tgcgcgtacc ttggttttga ttgttggagg ttaatactgc ggtaaatgtt gcatcggtta
    32521 ctggcgtacc atctgtgctt ttcacgaatt tagcgcgtaa aaaggcgagc ggggttagag
    32581 atgtaggatt ggtcacgagt gttgaatttg tctcccctgc taataaggta accgtaactg
    32641 agttggtggc aacatagttt cctgtttgcc gttttacgta atagtttcca ggcggtaatg
    32701 accactccat ttcaccctga gcatttggcg tccgtacacc gtaactttga ttatttgaag
    32761 ccaatacggc cgtaaacgta gcatcagtta ccggcgaacc atcagtactt ttcacgaact
    32821 ttgcccgtaa agtaccgttt tgcccgaagg cagaactgaa agccatgaga gccatcagtg
    32881 atgtgagaat cagttttttt gtaaggtttt tcatgcccta ctcctcattt tgtgaaacat
    32941 ggttaattaa aacgatagtt ttgtgctacc tagattaggt agtacaaaaa acgtaaaaca
    33001 tttcagaaat cttgacgatt atatgagtgt aataaataca cgaccacaca catgtgctgt
    33061 gtttgtgttg aaaaatgaat gggaaatggg attaattgcg aaatacgata taatacgttc
    33121 tctttatacc cctaaaaacg attttccttc taacacttca gtataaatga atctaaacgc
    33181 caaggcaagc aaaaaaagcc ccatttgaat ttcaaacggg gctaaaatat tctatgcaat
    33241 cttaaattct gacattatcc tcgcatcagc ggcttatact tgatacggtg cgggcgatcg
    33301 gcgtcaatgc ccatgcgctg tttgaagttt tcgtgatatt gcgaaaaatt tccgtcaaac
    33361 caaactacct ggctgtcgcc ttcgaatgct aagatatggg ttgctacacg atctagaaac
    33421 caacggtcgt gggaaatcac cacggcacat ccggcaaatt ccagaagcgc ttcttccagt
    33481 gcgcgcagtg tattgacatc cagatcgttt gtaggttcgt caagcagcag tacattggcg
    33541 ccttctttga gcatcttggc aaggtgaacg cggttgcgct cgccgccgga taacatgctg
    33601 acttttttct gttggtccga accgctaaag ttaaaacgcg ctacataggc acgcgaattg
    33661 acttcacggg tacccagcat cagcgtttct tcgccgccgc tgatttcttc ccaaatcgat
    33721 ttattgggat ccagtggacg attttgatct acatatccca gtttgacggt ttcgccgata
    33781 cgaatcgagc cggcgtcggg tttttccccg ccggtaatca tacgaaataa tgtggtttta
    33841 ccagcaccgt tgggtccgat cacgccgatg ataccgccgg gtggcagaga aaagttcatg
    33901 ttttcataaa gcaaacgatc atcgtaggat ttgccaacgc cgtttgcttc gatgacgaca
    33961 ttgccgaggc gcggtccggg tggaatgaaa atttccattt cttcgttacg cttttcattc
    34021 tgttcattga ccattttttc atacgccgaa atacgtgctt tacttttggc gtggcgacct
    34081 ttgggattca ttcgcaccca ctccaactct tcagccaaag ctttttggcg tttggattcc
    34141 tgcttttctt cgacttcaag gcgtttcttt ttctgttcaa gccacgaggt ataattgcct
    34201 tcgaaaggaa taccttcgcc gcgatcgagt tcgagtatcc aaccggcgac attatcgagg
    34261 aaataacgat cgtgggtgac ggcgatga
//
LOCUS       DAOQGF010000026        34212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_567_length_34212_cov_7.288140, whole genome shotgun sequence.
ACCESSION   DAOQGF010000026 DAOQGF010000000
VERSION     DAOQGF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_34212_cov_7.288140"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK937_05635"
     CDS             <1..447
                     /locus_tag="PK937_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH32084.1"
                     /translation="SIGRLPFDDASADLIVMDNVLMFIPTTIQSILMLEINRILKEGG
                     VAYFNFPNSWNPFNYLQDIKYRINSIYHSVPRFHRLGYMSYLRKLKLLDWDFEVYGET
                     FHVKIMGKRKPSKFMQSTLSKMDEKLHDTPLKVFMSGCFVIASKRA"
     gene            453..1850
                     /locus_tag="PK937_05640"
     CDS             453..1850
                     /locus_tag="PK937_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH32085.1"
                     /translation="MHGTVFYFTVLKYLYFVLNFFRGVLVAKVLGPEYYGVLGFWQMV
                     QTYIGYGHLGIPYSVNLELALHRDGAHKYVAPGFWLVASLSLAIFVITSLSFYFFNFG
                     YEYYSSRYGFFALIGGILLIFREFFIDIHQVFGNLYRIVVGDILTVVVLLLLPLFFSG
                     ETLIYYSLLGFCLSSAISLLFLIRIRGVSIVGRPEFATCRTLLVAGVPMFAASMITYF
                     ATLIPRAVLSLGDDNHRLGLFTLSVNIANAVMLGINSISWAIYPKLLAKTNIASGQSD
                     LERHVSRAMRLYPVVVAGVVIFSSIGIKLLMVWLPQYEGVEHVVSVMLLSQIIVSLSF
                     GPATMLYSNKLYNNLMMIGVLACSAVFVSSVILYQINVNLIVFATCVAIGYIVYSFFC
                     LYIYAKKTGSINWLSWTQDNISFLLIVVVAYIGSVLHFNLLILYTGLFLIFLLSHYRD
                     LFELRSIALSLYRKT"
     gene            1847..3013
                     /locus_tag="PK937_05645"
     CDS             1847..3013
                     /locus_tag="PK937_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32086.1"
                     /translation="MTQSILSAIFKVSFFLLFIGGGFLAFVGVPFRPFFVVFLLIFAL
                     PLLPNKNIPISFYVFASIILISGYLNGSSSKEVLYFIRFFITPSLVLIFLRTVVGQFN
                     LTRIFRLFLFIGIIQLPVIIFQKLFYDELIVYSAVMIDITDFDFGSFYIANDPATTFY
                     LLCLIVILLFNSKSHQYITLPYFWVGYFAFTILITNARMAHILLALVLLAFFLMKMSL
                     KNILSGAVLLVFIVISLYFSGFLDQLLENFQDLVWRITFDVDEYSSYENFAAGRYSRE
                     AAVLYYLNEPLKFFGDGPSKYYDPVTQEYLVGNNGLIFTFYSEIGLLGLGAGYMVIYS
                     IAREFSDKRVAFIPILFSFCMIGLTITTNVLSDMSIIFSFVLFLSINKKILYEN"
     gene            3003..4199
                     /locus_tag="PK937_05650"
     CDS             3003..4199
                     /locus_tag="PK937_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH32087.1"
                     /translation="MKTDALIFHRTLPHYRAEILGKLAEVMSFRWIHCSDQIDGLSSS
                     SANSHLAEKTSSITVRYFRNKGWTGENIIFMDVVTPILKYKPRFVFIQFELSMVSIWL
                     LFLIRKLYGIRIVLWGHGYDRKEIFDPRRSSKDKLRLYMMRQADAVLFYNANRKKDIL
                     SVLPSEEIAGKMFVANNTLNTDVLFQQYLSLQRDDFDVIKRKIGFTAKYNLIYIGRIV
                     QDKEPLFLIEVMRKLLISGLDVHLHVIGRGSLQSNMQALIDQYEMKPFVKMYGEILDN
                     KELGPFLYASDAFVMPGFLGLSIVHAFCFRCPVISKKTMPLGPFHSTEIEYLENGETG
                     LLLDHDIDRFTEGLYKFLVEDKERERMKKKIDEKIASACRIQVMQAGFQEAIRFINMQ
                     YEMSGD"
     gene            4199..4969
                     /locus_tag="PK937_05655"
     CDS             4199..4969
                     /locus_tag="PK937_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNH32088.1"
                     /translation="MAKIVGIEFYDQGIHRAVNVILNECKTIQKSNRIVSATDAHGIV
                     RSHSEREFKELLESFYLRLPDGMPTVWVGRIKGHSNMGRCYGPDFFKLVMQTSSTTPV
                     RHFLCGGKQGIAEQLRRVCSDRFGNNHVVGCYSPPFRDMTDEELRDLANQINDTSADI
                     VWIGLGSPKQEYFARRLSGYIGTHFLVTIGAAFDFHTGNIKQAPLWVQKLGLEWFFRL
                     CVEPRRLWKRYLYVVPRFFILGMHDIFIYLLHRRFGRL"
     gene            4987..8403
                     /locus_tag="PK937_05660"
     CDS             4987..8403
                     /locus_tag="PK937_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNH32089.1"
                     /translation="MTENYRGYIHTRGITSSVFLYICFAVVLLFPFSRVCPQEVVPLE
                     LFSKKIFDNYSYLNGSSDKKESLENYFLSGFNELIVPSTLVEWESRRNRIVDSLKQFY
                     KLDQWLSAQYPLNAIYHDTLDRPLYSIVLLTFQSYDGVRVPATLYLPKNGLEKKPAVV
                     YAEGYYFDAYGYNFANVDHWQRMSQHLALRGYVVLSINQLGLGQSALAGDTYQYQGYK
                     FNFAGDAFKVRSRLAPFIQGINYLFSREDVDTTFIGITGYSAAGAIAVEVGAIDQRIA
                     CTIVNDGGIYKTKLINESERIDPRSTTFSGSEASIDDFVLFSLFPPRPMIFIDSNQEL
                     YENYIPAALDRFKKLYEMYNTASGFHFLTNVGNHNYNNEKRLIAYSYFDTILGLPAGL
                     NEHDPSIELFDTLFHPSSFLISDFETKNTDEILSEMIYANSLKSVSDLEDYDISEAYS
                     KLGVDTSWSVISVSVKDSLSSGPYTVLYETIVLKEPLRDGSKLEYPVVTLKRNDKRPK
                     NKVFFVAENEIGREVWKTDSSLSRQLTNLLDSGFIVTGIDLLGYGELDIQFPSTNSLN
                     GSGYYKEQWTTEWVGYSGQLLMGHRISSLIAILKYLEDNRADISSVLWGYGFSPSLVV
                     QFVANKYPKTGTTILSNYLFDWNQLLNTTYGYYPDLYEANLLKTIDPSAIIAKSRVPL
                     LIQDIMNETRVKVTEGYRSRFESIRGSYFFGPELARSELLNTQLNFDYDISDTLRFWL
                     NNKPQIISNYFFNAAEDAYFHSNILVNDPDTLLYESELRYRFDNLPQWLILDSVRGFF
                     YGTPTANDIGSYVIKLQVTDVAGDYDSASITLNVVHTNHPPLWRSEFKTDAKEGLIYD
                     AYVYASDKDSALFGDTVKYEILGGPKWMEIDSLTGKMSGMPPNNTHGDLPLNLRAKDQ
                     FGGFKDTMIVIHIEPTNYPPPVPIAVFPAMGDTITIFRKPLVYRFIWRASIDPDNEDT
                     VNYLLHFTGNNLDTMIKVKSDTSLDFDSAVLNEMSEYSLKLYAVDDEFSSMSDSSVTF
                     FTTQAYANRNYVDVPTDYKLHQNYPNPFGHMTNICFQIPELSNVTITFYNTIGQFVDR
                     IVLEKVEPKYYEVKWFATAKSSAVYFVRFEASSFVSRKKFQKVMKILHVK"
     gene            8459..9469
                     /gene="gmd"
                     /locus_tag="PK937_05665"
     CDS             8459..9469
                     /gene="gmd"
                     /locus_tag="PK937_05665"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH32090.1"
                     /translation="MNKKALITGITGQDGSYLAELLLEKGYEVHGIIRRASTFNTDRI
                     DHLYKDPHVNGVRMFLHYGDIADSANLIKLIHQIEPDEIYHLAAQSHVRVSFDIPEYT
                     GDVTGVGTARILEAIRMTGCKARFYQASSSEMYGLVQEVPQKETTPFYPRSPYGAAKV
                     YAYWLTVNYRESYNMFATNGILFNHESPRRGETFVTRKITRAVAHIAAGLQNKLYLGN
                     LDAKRDWGYAKEYVEAMWLMLQQEKADDFVVATGETHTVREFLEVAFGYVGKNYQDFV
                     DIDPVYYRPAEVDLLIGDASKARQILHWQPKTTFRELVELMVEADISLLEQTHKINIR
                     KK"
     gene            9469..10395
                     /locus_tag="PK937_05670"
     CDS             9469..10395
                     /locus_tag="PK937_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNH32091.1"
                     /translation="MKKNDKIFVAGHKGLAGSAILRKLIDQGYTNLVLRTHQELDLEN
                     QTDVRVFFEKERPEYVFLAAAKVGGIHANNVYRADFLIKNLKIQNNVIESAYRFGSKK
                     LLFLGSSCIYPKLAPQPLKEEYLLTGLLEPTNEPYAIAKIAGIKLCESYNRQYGTDFI
                     SAMPTNLYGPNDNFDLNNSHVLPALIRKFVEARDRNASEVTIWGTGTPLREFLHVDDL
                     ADALIFLMMHYSGNEFVNVGTGREISILDLAHCIKRLTAYSGKLEFDSSKPDGTPRKV
                     MDISKLNAMGWYPKISLDTGLKQTIDWYEKHV"
     gene            10488..11870
                     /locus_tag="PK937_05675"
     CDS             10488..11870
                     /locus_tag="PK937_05675"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNH32092.1"
                     /translation="MLYFLRVTADTVGVLVAALSSIYFFSIRDARITTNFDLFLLTSL
                     AFSWYFLARMTGLYDEFRSRNFSYELVGLIKNILFQIVIAIVVMFIAKNHTLPRLFIV
                     LYAAHLLIILSIERFLVRRVLNFLRRKGRNLRHLLIIGAGEVGTAFNELIRSNSHFGY
                     AVTGFLDDQPQVSLNGKLLGKVEDLESVLQKHPVDDVIVALPTYASEKIEWIIKICDH
                     HTRRVRIIPDYFRFVSDKFEVRMFGRFPLISVRKIKMDEAHWYIIKRCFDAFFALAFM
                     VLVLSWLVPIIAIAIKLTSRGPVFFKQERWGRQNKKIICYKFRTMISQSRDVDDSGKY
                     QQATQDDPRITPLGRFLRKTNLDELPQFWNVLMGQMSVVGPRPHPTPLNMESKEVIEN
                     YMLRHLVKPGITGWAQVNGYRGETRDPELMQKRVNHDIWYIENWSFWLDIQIIFLTVW
                     QMIKYDTKGY"
     gene            11870..13555
                     /locus_tag="PK937_05680"
     CDS             11870..13555
                     /locus_tag="PK937_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNH32093.1"
                     /translation="MLALHSQKYIVATGLAVLVAAFLMPCPGWAQYTGPSVNLPMGHF
                     GYDLIEHLETRGVVSPSIRLRTKPYDRGTVLKIINEVQDKIKTDANVLTSAERSLFEK
                     LKGEFYVELTSTQNIQTSDVREKHIFEYQTHEDGFSYLVGDLVIDQSVELTRFSKKND
                     SLGGTIGLSKGSGRVRGLIKGGIAFYADASNTLVKGDEQSTDFGSSYRGGVLNYNPAS
                     ANRYALHSDAYIVIEPKWFRLQVGKDNMAWGPGYYDNLLLSGNAPSFENLRLDVTYNR
                     FKFSYVHGWLRDDRDTVNGRKDLSDRKYIVAHRVEWKALPWLFLSGSESVIYGGRDVE
                     LSYLNPLVPYHVAEQYLGDKDNNTMSFDVLAFLPGRTKVYGAVYLDDFTSARNPFTYW
                     KQTWAVMGGGYWVEPMGFKDAGVRIEYSRIEPFVYAHKWRLLNYTHFDAGLGSQLQPN
                     SDRWNFLISYQYSRRLRAEVGFRRIRHGEGDIQRFGYEYGFTDDDSGKQKKRFLMGIQ
                     EIRNSALFNASFETWSHHALYLQYELSMIRNLGNRKGFDATQNYLRIGYTLDY"
     gene            13611..14519
                     /gene="rocF"
                     /locus_tag="PK937_05685"
     CDS             13611..14519
                     /gene="rocF"
                     /locus_tag="PK937_05685"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNH32094.1"
                     /translation="MSVIRLIGVPLDLGAGRRGTDMGPSALRLAGLTERLQELGHTVK
                     DKGDIVTKNPEVIRVGDEKLKYLNEVTRAVTALADDVEKCLADNHIPLVLGGDHSIAI
                     GTLAGVSGYFRKNNQKIGVIWIDAHGDMNTPDTSPSGNIHGMPLAVSLGHGHPKLTGV
                     GGEFVKVNPENTVLVGIRTLDDGEIDNIRRMGVTVFTMRDIDEQGMSKIIRQAIDIAA
                     RDTAGIHVSFDADSMDPSIAPGVGTPVPGGLTYRETHLAMESLCDSKKVISAEIVETN
                     PILDIKNTTAHVGVEMMASLFGKRIM"
     gene            14558..17203
                     /locus_tag="PK937_05690"
     CDS             14558..17203
                     /locus_tag="PK937_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNH32095.1"
                     /translation="MIFRIITLLCCALFVQAQNDFRVLGEKNGYDPQTLREYVNLTET
                     RAFLDLAGEWTMSVEGVSRKVIVPSSYDYNGKVTFTRTFVPGEDFRNKHFRFVSLGIN
                     FRADIRINGEFVTNANNSYTRLEADLREDLIRIGQENKIEITVDSRVDPFTSYPRETS
                     LLQPKLYGGITRELFLVGLPKVAIDQYTVGYTLSNDLRKCDFVVNWHLRNYEYSLRLV
                     DTLRLINPNQPLRYILEMFEENNPEPVYTNRFKKYRNVWDIPKIREQNEEDTVSVFNF
                     VDRQLRFSIEKPVFWNPITPSRYRVVMYLVQGTTVIDRVEYQLGIAQADVHDQYIFLN
                     AQPVTIRAVEYYEDYPGAGSTMSWAMMEQDILKIKRLGANAVSFRHHPPHPYFVELCN
                     RYGLLLFYEIPSWRLTADIMRTPSYVEDIRKYYRHIVEYEHLNPSILAWGVGFAPDPS
                     DGAANYYFQQIVGEIKNKDRRPVFAASAFGGYDSFMDNVDIVQLDIRLNSPLSSNLFV
                     QRSLAKWPTRALIAVYGAQIFSNNQNGYSDPMSTKFQAKVLADFYKKTGEWKLAGGVV
                     RSFNDFAVQRPYLYANPNASSTIYTSGVVTYDRQERYAFETLRALYSDDALEPIAVGA
                     YEPVYPKTYPITGLILVVLFISFYRQSPKFKNSVHRSLFKVVSFFGDIRENRVITLWP
                     ALLVGFLASLAYAALLSIVFFELRRNHLFDEYLATFLRSNGLKAWVDGLAWSPELFVL
                     EWTMIYALVITVIGLLFMIFQKPFQAYVNFQQSIIAAAWSSSHFLYLIPFVVVFHRLM
                     KTDFVLSLGATAAVLFFFWHWIRFFRAAQILFNASWWRTGFILGGALIVLCTALGIYY
                     SGSHDFFNVWNYIEQVSSSKNYTTE"
     gene            17200..17784
                     /gene="pyrE"
                     /locus_tag="PK937_05695"
     CDS             17200..17784
                     /gene="pyrE"
                     /locus_tag="PK937_05695"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNH32096.1"
                     /translation="MMKQEEVLGIFKQSGALLEGHFILTSGAHSPNYFQCARVLQHPN
                     WAQLLCSEIALHYKDKKIHAVAAPAVGGIIVAHEVARTLNARCIFSERENGTMRFRRG
                     FEIKRDENVLVVEDVVTTGGSLKEVIQLCRDADANVVGVGYLVDRSGGRAEFGVPKFS
                     LVSVDVVKYEPETCPLCREGALPAIKPGSRSMNK"
     gene            17861..18691
                     /locus_tag="PK937_05700"
     CDS             17861..18691
                     /locus_tag="PK937_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH32097.1"
                     /translation="MLTEANNAVRVCVLGSGSKGNSTFLQYGATKVLIDAGLSGEETM
                     DRLTAVATPINELQHIFITHEHDDHIKGLKKILKSFSGMVHCNAGTFRHLRHRIPEQT
                     RVHVFSDTFEADGLQVQPFSISHDATEPVGYSFQCGHRQVTVATDMGYASELVKARIQ
                     DSDILILESNHDEGMLMKGRYPWPLKQRIASRVGHLSNRQAGELMRDTMFPRLQWLVL
                     AHLSEHNNMPDLAMASMTEHLQTTAMKEQIQVHVAHQHTISAVMELNHSNVTETAHHV
                     "
     gene            18684..20495
                     /locus_tag="PK937_05705"
     CDS             18684..20495
                     /locus_tag="PK937_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32098.1"
                     /translation="MSENTKITNTQDITDPERLIETDDKFSKSDHVHYKEILHRYYRQ
                     SLPFDIQSYKNLDEQPPDARIAMKNDPFFRDVGKYVHILDFSEQERIKFGRENEVQHL
                     IVFLLFERDLDVLEAVFGNPQLPTKVLYDFINLIKERDIDREDDKILKLAQKVMKRRS
                     RRIVKVREIHTANTQPIQRANLITLFSYLIDDDAQVRMAAENVVSALDVQTLINALED
                     DSLIHTMRESISKITAISIFRMLESAVHLEIRNLAAGGDLGADQTNEHNAARRQLTQV
                     LQDRKRRELEKCVEDPTDLFNATLITYLHFDKDADLRAQALEILQPDDIIDLVSDDST
                     PRHISSQVLPMLEKSTDERVRQRTTEIRLKEAERLNKKMKEIEVSVNAYFDVIFQSLN
                     YSRINSQKEAVTMLRGSLNYLQLFAKEAKNLESGSATVAQGVIRQAIEHFEGSINALY
                     SDTKRELFTEFEEIQGMVRHILDLKSFKFEEESGVEDEVDEAVLNKAVMIWRNTISQF
                     LGRIKDLEEMLRIKWGKLMTENEPKRKQEENESDLYEAFGEIETMHKNEVDCKLKIPC
                     RECKRRGCASERFMLQVDFLLEEIHQGFKHDKQTHHA"
     gene            20488..21063
                     /locus_tag="PK937_05710"
     CDS             20488..21063
                     /locus_tag="PK937_05710"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNH32099.1"
                     /translation="MPKPLPRSFYARPTLEVAPELLGKILHHGTMSARIVEVEAYRAG
                     DDPASHAYRGLTPRNKIMFGVPGHLYVYFIYGMYYCANVVCEKEGTAGACLIRAVEPL
                     DGVERMQANRVMARGFALSNGPGKLCLALGIDKSHNGTDLTTDDLYLSDDRYHAKIKT
                     SPRIGIRVGTEKKWRYFIDGNAWVSPSRNNK"
     gene            21373..24069
                     /locus_tag="PK937_05715"
     CDS             21373..24069
                     /locus_tag="PK937_05715"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNH32100.1"
                     /translation="MSEAQQKTEIPKAYDPKASEDKWYAYWLKHDFFKAHADSAKPVF
                     TIVIPPPNVTGALHIGHAYNNSVQDIYTRFKRMQGFETLWTPGTDHAGIATQTVVEKQ
                     LAKEGTSRKTLGREAFVNEVWKHKETYRARIVGQLQKMGCSCDWEREWFTMDEKLSEA
                     VKEVFIHFYNKGWIYKGQYIVNWCPYSQSAISDEEVEYKEINGKMYYFRYPVKDSAEH
                     IVIATTRPETMLGDTAVVVNPDDARYKHLIGKKVILPLVGREIPIIADDYVDKEFGTG
                     AMKVTPSHDPNDFVLGKRHQLQFLNIMHADATMNQNVPEKYRGMERFAARKAVVADLE
                     SAGLVEKIQDHLNRVGYSQRGNVPIEPLVSEQWFLKMDELAKPALEAVLKGDIRFHPE
                     KWIKTYEHWMTSIRDWCISRQLWWGHRIPAFYAPDGSVAVARNADEALAILKKKNAQL
                     TVSDLRQDEDVLDTWFSSWLVPFSSMGWPHQTKDLEKFYPTSLLVTGPDIIFFWVARM
                     IMAGLEFTGKVPFCQVYFNGIVRDELGRKMSKSLGNGIDPIAVIDKYSADALRFTLVD
                     LSAEGQDINLAERQFELGRNFSNKVWNAFRFLANYQEQLPSAKPQLPPSNAITELADR
                     WILSRYHRAVEGITTGLENYKLHEAMDSVYRFFWADYCDWYLELIKDRMYNATPEIKE
                     KTLSTALYILDGVLRLMHPIVPFVTEEIWQRLMPRTDGESIMIQPWPQCEASYISVEA
                     EKDLEQLRDTITTIRNIRSEMNIAPSKKTDLVLRCVTPQDAALYNANTSALAGLARVE
                     KITAAVGADKPRIAASAVVNGTEVYLPLEGVIDLEVERTRLQKEIDRISGQIGGIEQK
                     LGNEGFVAKAPPQVLEKERAKLDNFRATVEKLRSNLSALQNL"
     gene            24118..25002
                     /locus_tag="PK937_05720"
     CDS             24118..25002
                     /locus_tag="PK937_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNH32101.1"
                     /translation="MTAWTERLRDAKPLLLDGATGSELERRGVPCVLPLWSAVALLQN
                     EYTSVLRAIHAEYIQRGAQIITANTFRLNRQTLMTEQLHNDSRRLICKAVDTVREAIE
                     SVARDRTVFIAGSIGPVEDCYHPERAPADDVLRDDHHRRIDDLYAARPDLILIETMNN
                     MNEASIATEYAVQTGLPVAVSFIARNDTQLLSGESLEKAIRRIKTYNPAALMLNCMHV
                     EIMEHALDVLKDTAGDIPIGAYVNIFPEQSKPAMTPEDFASRMEAWVSEYKLKIIGGC
                     CGTTPRHIEKLAERIGVN"
     gene            25083..28331
                     /locus_tag="PK937_05725"
     CDS             25083..28331
                     /locus_tag="PK937_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH32102.1"
                     /translation="MLLWMNVTFLAAQTERLYVERISVEQGLSQTTIFGMMQDRRGFM
                     WFGTEDGLNRYDGYQFRIFKNDPFDSTSLSNNYINTLYEDRRRFIWVMTLEGVNRYDP
                     RDEKFLRFKRSDDTTTIAGYRIENMAEDSVGHLWCTTDRGMLLRVDENGKIQERWNPR
                     SADDMSSVQNLQIENGNRFFLSTNSGLYICDPTRRHVVRYHREAAGDYRLSENSVRSV
                     VKDRRGWYYVATYGGGLYMYDEVWRLQNNFKKADASKHGLTEDDLQTLYCDRAGRIWV
                     GSEGGGLFLFSPEQNKFLQFKTESGDAHSLSNNLILRLTEDRTGVIWIGHRDGGISKY
                     DPQRIRFVSYRHDPDHTNSLSHNSVYSIFEDPADSGKVLWIGTNGGGLNRWDRITQTF
                     RSYVNTPGVRHTIGDNSVRFLFKDSEGVFWVGTNKSGLQRMDTDRGLFEPSTNPNIKF
                     IRYVIEDRKDKNIFWVGTNNNGVFRIEKSGKILQQYSPQTKPGLSFNIIRGLYQDSDG
                     KVWVATQGGGLNCISADGQSVTVYNYDPTNPQSLSNSIVLCILPDTTQNIFWLGTAGG
                     LNRFDSRTGITQHFRERDGLANDVVYGVLRDEKGFLWMSTNGGLSKFDPKTSVFRNYD
                     TKDGLQSNEFNAGAFFKNQNGEMFFGGIQGFNVFHPDRITDNTMPVSVVLTQFQIANQ
                     PVAVDPNGVLKQSVTESQQIVLPYAGNAFAFEFSALMYHAPEQHQYAYRLEGFDKTWL
                     YSGTRRYVSYTNLDPGEYIFRVKASNHDGVWNEDGASMMIIITPPFWQTWWFRLLMLG
                     FAGLIVWGMLKLRSYYAEYRKTHYVSHYKLLEKIGEGGMGVVYKARDRVSGKTVALKL
                     LHESIADTKDGLQRFLKEAEVGKLIHHPNVVKIYGAGSSETRRFITMEMVEGKTLKQT
                     IRERGVFTKDDTKKIIIQLVEGLAQIHVHGVVHRDLKSDNIIIKPDGRVVIMDFGLAR
                     IKGMTSVAARDQLVGTLAYMSPEQTIGKEVDHRSDIYSLGVITYEMLYGEMPFIANNE
                     MELILAIHNETPKHIDANNVDALHQCIKICLSKDPIDRYPDTTTLRKFLDTV"
     gene            28396..31677
                     /locus_tag="PK937_05730"
     CDS             28396..31677
                     /locus_tag="PK937_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNH32103.1"
                     /translation="MSSAQYQLPFVHYTADNQINPLPGVAVYDVYQDRLNFLWISIFS
                     AGLVRYDGHRMEVYTTRDGLRSLNFTTTIEDTDGRLWVMTDGGLSVSEQPLKTYGAHR
                     VRFIDRIGDQPLAKTSISQIAMKVLAQDTAGRIYLGTTGAGILRYSRVKPDFWSCDTI
                     TTQNSHSSRNASVYSMMVDKKGLVWISTDDGVAILKPGGHRIEYLERSIIPPVRVQTF
                     FQTNDETLLAGCEDGSIYQWEKEAHGFRKMDHTLTSPVYDITQDRRGDLWICSDGAGV
                     LRWRKGNSPEHITRRQGLIAESVRFVMEDREGTLWFSQVGGLSKLRANYKAFQSISAD
                     ATLETGVVLPDPNVTGISTEAWTGDKPLLWMGLGKGFAVLTHDGKISHFDAKKGLSSN
                     TVYDVLVDEKKRLWVGTFAGLNSFTFYGTPPVTLGATSSRRVQLNNQPAVMDFHDIGI
                     TGVVSRYAMRENPEAQRTVESLWFNSIRRLSVYVGNSWYIFDEASGLPSSIIYSIAWD
                     SAGWMYVGTGDQGIYRSRFSLTSSELRKQFERSGKGLYQREITTPCFEKYWNQENGAP
                     FDDPQSLLFVGDRLWVGSASRGLVVINPQTRTLIHQMNSDAGFRSPGIINLIFSPVFG
                     TVWCGSSNGIYEIDPISYKLLRSVFRDDGLISSEVNWLTSTAFDSDGKLYIGTPKGMT
                     IYDPKLDRPDLTSPQPSITSATFHQSLGGENELLTEFSALSFTNEKAVQYKVRLVGYE
                     SDWHAVSEPRTRYTNLPAWFTEARYRFEVLASNAHGVWSEMPAVYEFNVMPVWWLRWW
                     AFGIYIALFSALVLGARWFVINWRSVLMPKTRYIAHYKILEKIGRGGMGMVYKARDTL
                     NKRTVAIKILHEEFEQTEDGIKRFIKEAEIGRKLKHPHIVEIYDAGRFDKTRYITMEF
                     IEGVTLKNFLRVHGPLMPADVVRIIRPVAQALRTIHAHDVIHRDLKSDNVMIRNDGVV
                     KIMDFGLARTKGMTTVVQRDQLVGTLAYMSPEQSLGKPVDARSDIYALGVIMFEMVEG
                     CLPFNGQHEMELIYAIHNETPEFHNTNIPEALRQFILRCLEKEPERRLANMDELEEVL
                     NKIAV"
     gene            31744..33486
                     /locus_tag="PK937_05735"
     CDS             31744..33486
                     /locus_tag="PK937_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNH32104.1"
                     /translation="MIKIYESLQDLQVKIDETFKELSQGFNKVNGAITQKNLPAAESA
                     LNDLFKAFMSRTRAINAQSASVGADIKKIESQCAEISAEKEQFKALYNSGIAMTTQGE
                     LQGLITFAMNQMTYHLRAERGFLILVDDHGEREYFVSKNFDGQNIDDPAAEVSTSVIQ
                     KTLDALQPIKVDDDTLSESIMKQGSFVRLGLRAVMSVPIIFRKKLLGVVYLDRREETK
                     GFSDNDLAFLMAFSRQIAFRVNELKEQQHVHTEYERRDRNRLQSLREKYNFSEIIGKS
                     ERLVKVLELAGKVASSEVTVLIQGESGTGKELIARAIHFNSDRYEHKFIAINCGAIPS
                     ELLESELFGYESGAFTGAVKTKIGKFEAAHRGTLFLDEVAELSMNLQAKILRVLQTKE
                     LERLGSTETRKIDLRIISASNKNLQQMVREGKFREDLYYRLKVVEIELPALRERKEDI
                     HLLVSFFVQKYGEQRIQSVSEDAMDILENYAWHGNIRELENVIQRAIILSSSTQIEVQ
                     DLPPEIVSKTESGYKVDKDLSLEDAETDFRRWFVLRALRKSNNNKSKAAEMLGINRTH
                     FFRMLNQLGISDSE"
     gene            33687..33896
                     /locus_tag="PK937_05740"
     CDS             33687..33896
                     /locus_tag="PK937_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32105.1"
                     /translation="MKKKVLIFNQDIDELSVIRQVLVREGYEAITASNWDTATKLFAS
                     LEIDYVLMNGKSDDIQRVLQSALKV"
BASE COUNT         9327 a         6714 c         8091 g        10080 t
ORIGIN      
        1 tctattggta ggttgccgtt cgacgacgct agtgccgatc ttatcgttat ggacaatgta
       61 ttgatgttta ttcccacaac aattcaaagt atactgatgt tggaaataaa cagaattcta
      121 aaagaaggag gggtagctta ttttaatttc cctaactcat ggaacccttt taactactta
      181 caggatatta aatacaggat taattccatc tatcatagtg ttccgagatt ccatagactt
      241 ggctacatgt catacctgcg gaaattaaaa ttgctagatt gggactttga agtctatggt
      301 gaaacttttc atgttaaaat aatgggtaag agaaaacctt ctaaattcat gcaaagtacg
      361 cttagcaaaa tggacgaaaa actacatgac actcctctga aagtttttat gtcgggttgc
      421 tttgttattg cctctaagcg agcttaaata ttatgcatgg aacagttttt tatttcactg
      481 tccttaagta tttatatttt gttctgaatt tttttcgcgg agttttagtt gcgaaggttt
      541 tagggccaga gtattatggc gttttaggtt tttggcagat ggttcagacc tacattggat
      601 atggacactt aggaatccct tacagcgtca accttgaact ggctttacat cgggacggtg
      661 cgcataaata tgtagctcca ggtttttggt tggtcgcgtc attgtcactg gcgatatttg
      721 ttataacatc tctgtcgttt tattttttta actttggcta tgagtattat tcttcgcgct
      781 atggtttttt tgcattaatc gggggaatac ttcttatttt cagagaattt tttattgaca
      841 tacaccaagt gtttgggaat ttatatcgca ttgttgttgg tgacattttg actgtagttg
      901 ttttgttgtt gctgcctctt tttttctccg gtgaaacatt gatctattac tccttattgg
      961 ggttttgttt gagttctgct attagtttac tttttttaat tcgaattcga ggtgtttcaa
     1021 ttgtagggcg gccagagttt gcaacttgta gaaccctgtt agttgcaggt gtccctatgt
     1081 ttgctgcaag tatgattact tattttgcaa cacttattcc tcgtgctgtt ctcagtttgg
     1141 gcgatgataa tcatcgttta gggcttttta cactaagtgt taatattgcc aatgccgtca
     1201 tgttggggat taacagtatt tcatgggcta tttatccgaa gttgttagct aaaacaaaca
     1261 tagcgtcggg tcaatccgat ttggaaagac acgttagtag agctatgcga ttgtatccgg
     1321 tggtagtcgc tggcgttgtg attttttcat caatagggat taagctatta atggtatggt
     1381 tgccgcaata tgaaggtgta gagcatgttg tttctgtcat gttgctttct caaatcatcg
     1441 tgtcattatc atttgggcct gcaacgatgc tgtattcaaa taagctctat aataatctca
     1501 tgatgatcgg tgtattggct tgcagtgccg tatttgtaag ttcagtgatt ttatatcaaa
     1561 tcaacgttaa tctcattgtg tttgcaacat gcgtcgccat cggatatatc gtatattcct
     1621 ttttctgtct ctatatttac gcaaaaaaaa ctggtagtat aaattggtta tcttggactc
     1681 aggataatat cagtttcttg cttatagttg ttgttgccta tataggctct gtattgcact
     1741 tcaatttact cattttatat acaggacttt ttctcatttt tcttctgtca cattatcgtg
     1801 atttgttcga gcttcgatct atagcattaa gcttgtatcg aaaaacatga cgcaatcgat
     1861 actttcagcg atattcaagg taagcttttt tcttctcttt attggaggtg gattcttggc
     1921 ttttgttggg gttccgtttc gcccgttctt tgtggttttt ctactgattt ttgcgctgcc
     1981 attacttcct aacaaaaata tacctatatc cttttatgtg ttcgccagta ttatcctcat
     2041 atcagggtat ttaaacggct cttcgtcaaa agaagtattg tattttattc gtttttttat
     2101 aacaccgtcg ctcgttttga tttttttacg aaccgttgtt ggtcaattta acctcacaag
     2161 aatttttcgc ctgtttttat ttataggcat aatacagctg ccggttataa tctttcagaa
     2221 actattttat gatgaattaa ttgtttattc cgcggtaatg atcgatataa ctgattttga
     2281 ttttggtagt ttttatatcg caaatgatcc tgctaccacg ttttatttgc tttgtttgat
     2341 tgtcatactg ttgttcaact caaagtcgca tcagtatata acattaccat atttttgggt
     2401 tggctacttt gcttttacta ttctgataac taacgcacgc atggcgcata ttcttcttgc
     2461 tttggttctt ctagcattct ttctaatgaa aatgtcgtta aagaatattt tgtcaggtgc
     2521 ggtattgttg gtgttcattg ttattagttt atacttttca ggcttcctag atcaattact
     2581 tgaaaacttt caggatcttg tttggcgcat aaccttcgat gtggatgaat actcaagtta
     2641 tgagaatttt gctgcaggaa ggtactcacg cgaagcagcc gttctttatt atttgaatga
     2701 gccattgaaa ttttttggtg acggaccatc aaaatattat gatcctgtta ctcaagaata
     2761 tcttgtcggc aataacggcc tgatttttac attctattct gaaattggtt tattaggact
     2821 gggggcaggc tatatggtga tatattctat tgctcgtgaa ttttccgata aaagggttgc
     2881 ttttatacct atcttgtttt ctttttgtat gattggtttg actattacca ccaatgtgct
     2941 tagtgatatg agtattattt tttcatttgt tttattctta agtataaata aaaaaatttt
     3001 atatgaaaac tgatgccctg atatttcacc ggacgctacc acactatcga gcagagattc
     3061 ttggaaaatt agctgaagtg atgtccttcc gatggataca ttgctctgat cagattgatg
     3121 gattgtcgag tagttctgcg aattcgcatc tagccgaaaa aacaagttca ataaccgtgc
     3181 gttactttcg gaataagggt tggacaggcg agaatattat ctttatggat gttgttactc
     3241 cgatcctaaa atacaaaccg cgtttcgtct ttattcaatt tgaattaagt atggtttcta
     3301 tctggttatt gtttttaatt cgtaaactat atggcattcg cattgtattg tggggccacg
     3361 gttatgacag aaaagagata tttgatcccc gtagaagttc taaagataaa ctgcggctct
     3421 atatgatgcg ccaagctgat gccgtattat tttataatgc taatcgaaag aaagatattc
     3481 tttccgtctt gccttcagag gagatagccg gtaagatgtt tgttgctaat aataccctta
     3541 atacagatgt tctttttcaa cagtatttgt cacttcaacg tgacgacttt gatgtgatta
     3601 agcgtaaaat aggatttact gcgaaatata atttaatata tatcgggcgt attgttcagg
     3661 ataaggaacc gctttttttg atcgaagtaa tgagaaagtt actcatttcc ggtttggatg
     3721 ttcatttaca tgtgattggg cgtgggagct tacaaagcaa tatgcaggcg cttatcgatc
     3781 aatatgaaat gaagcctttt gtgaaaatgt atggagaaat attagataac aaagaattag
     3841 gacctttttt atatgcaagt gatgcatttg taatgccggg ttttttggga ttatctattg
     3901 tacatgcatt ttgttttaga tgtccagtta tttctaaaaa gaccatgcca ttggggccat
     3961 ttcatagtac agaaattgaa tatttagaaa atggtgagac tggccttttg ttagatcatg
     4021 atattgatcg ttttactgaa gggttgtata agtttttggt ggaggacaaa gagcgggaac
     4081 gaatgaagaa aaaaatagat gaaaaaatcg ctagtgcttg tcgaattcaa gtaatgcaag
     4141 ccggttttca agaggcaata cgttttataa atatgcaata tgaaatgtcg ggtgattaat
     4201 ggccaaaatt gtcggaattg agttttatga tcaagggata catagagccg taaatgttat
     4261 attaaatgaa tgcaagacga ttcagaaatc taatcgaatc gtttctgcta cggatgctca
     4321 tggtatagtg cggtcacata gtgaacgcga gttcaaagaa ttattagaat ctttttattt
     4381 aaggctccct gatggaatgc ctacagtttg ggttggtcgc atcaaaggac attccaatat
     4441 gggtcgttgt tacggccctg atttttttaa gttagtcatg caaacttcaa gtacaacacc
     4501 agtgcgtcat tttttgtgtg gaggtaaaca aggtattgct gaacaacttc gacgtgtttg
     4561 tagtgataga tttggcaaca accatgttgt cggttgttat tcccccccct ttcgtgatat
     4621 gacagatgaa gagttgcgag acttagcaaa tcagatcaat gatacatcgg cggatattgt
     4681 ttggataggt ttaggtagtc cgaagcaaga gtattttgca cggaggttgt ccggatatat
     4741 tggcactcat tttttagtta cgattggagc ggcatttgac tttcatacag gcaatataaa
     4801 acaagcgcct ttgtgggtac aaaaactcgg ccttgagtgg ttttttcgat tgtgtgtgga
     4861 gcctcgtcgc ttgtggaaga gatatttgta cgttgttcct cgtttcttta tacttggaat
     4921 gcacgatata tttatctatt tacttcaccg ccgttttggc cgcttataaa gcaaaatgtg
     4981 aagaatatga cagaaaatta caggggatat atccatacac gcggcatcac ttcttctgtt
     5041 tttttatata tctgtttcgc cgtagtactt ctgtttcctt tttccagggt ttgcccacag
     5101 gaagttgtgc ctttggaatt gttttctaaa aagatttttg ataactatag ttatttaaat
     5161 ggctcttccg ataaaaaaga gtcgctagaa aactattttc tcagtggttt taacgaatta
     5221 atagtaccat caacgttggt cgaatgggag tctcggcgaa atcgcatcgt tgattcgtta
     5281 aagcaatttt acaagctcga tcaatggctt tctgctcaat atccgctcaa tgcgatttat
     5341 catgatacac tagatagacc gttatattca attgtccttt taacttttca gtcttatgat
     5401 ggagtcagag tgccggcaac tctttatctt ccaaagaatg gactagaaaa aaaaccggca
     5461 gtggtgtatg ccgaagggta ctactttgat gcctatggtt acaattttgc aaatgttgat
     5521 cattggcagc gcatgtcgca acatctagct ctgcgcggtt atgtagtgct aagtattaat
     5581 cagcttgggc ttggtcaatc cgccttagcc ggagatactt accagtatca gggttataag
     5641 tttaattttg ctggagatgc ctttaaggtt cgttctcgtt tagctccttt tattcaggga
     5701 attaactatc ttttttcgcg tgaagatgta gataccacat ttattggtat aacaggatat
     5761 tcggccgctg gggctattgc tgtcgaagta ggcgctatag atcaaagaat agcttgtact
     5821 attgtaaatg atggtgggat atataaaact aaattgatca atgaatctga acgcatagat
     5881 cctagatcta caacgttttc aggttctgaa gcaagcatag atgattttgt tttattttct
     5941 ttatttccac ctcgccctat gatttttatt gattcaaatc aagagctata tgaaaactat
     6001 attcctgctg cgcttgatcg ttttaaaaaa ttatatgaaa tgtataatac cgcgtccggg
     6061 tttcattttt tgacaaatgt aggcaatcac aactacaata acgaaaaaag gctgattgct
     6121 tattcgtatt ttgatacaat attggggtta cctgcaggtt tgaatgaaca tgacccatca
     6181 attgagcttt ttgatacatt gtttcacccc tcttcttttc ttataagtga ttttgaaaca
     6241 aaaaacacgg atgaaatctt atccgagatg atttacgcaa acagtttaaa atcggtatcc
     6301 gatcttgagg attatgatat ttcagaagcg tatagtaagt tgggtgtaga tacatcttgg
     6361 tctgtaatca gtgtttctgt taaagactca ttgagtagtg gcccatatac cgtgttatat
     6421 gaaactatcg ttttaaaaga gcccttgaga gacggttcta aactggagta tccggtagta
     6481 acattaaaga gaaatgataa aaggcctaaa aataaagttt tttttgttgc tgaaaatgaa
     6541 ataggtcgtg aagtatggaa gaccgactca agcctttcga gacaattgac aaatttattg
     6601 gattcaggtt ttattgttac tggaattgat ctcctcggtt atggtgaact ggatatacaa
     6661 tttccttcta caaatagttt aaatggatca ggctattata aagaacaatg gacaacggaa
     6721 tgggttggtt attccggcca gttattaatg ggacatcgga ttagcagctt gatcgcaatt
     6781 ttgaagtatc ttgaagataa tcgcgcggat atctcgtccg tattgtgggg gtatggtttt
     6841 tctccatcgc ttgttgtgca attcgttgca aataaatatc caaaaactgg aacaactatt
     6901 ctatccaatt atctgtttga ttggaatcaa cttttgaata ctacgtatgg atattatcct
     6961 gatttgtatg aagcgaattt actaaaaacg atagatccga gcgccattat tgctaagtct
     7021 agagttccat tattgattca ggatatcatg aatgaaacta gagtcaaagt tactgagggt
     7081 taccgaagtc gatttgaatc tatacggggt tcttattttt ttggacctga attggctcgt
     7141 tctgaacttc ttaatacgca gctcaacttt gattatgata tatctgatac ccttcgattt
     7201 tggcttaata ataaaccgca aattattagt aattattttt ttaatgcagc tgaagatgct
     7261 tattttcatt ctaatatttt agtaaacgac cctgatacac tgttatatga atctgaattg
     7321 cgatatcgat ttgataacct cccacagtgg cttatacttg attcggtgag aggttttttt
     7381 tacgggacac ctacggcaaa cgatatagga agttatgtga taaaactgca agtgacagat
     7441 gttgccggag attatgattc ggcatcaatc actctcaatg ttgtccacac aaatcatccg
     7501 cccctttgga gaagcgaatt taaaacggat gccaaagagg gcttaattta tgatgcttat
     7561 gtttatgcat ctgataaaga tagtgcttta tttggcgata cagtaaaata tgaaattctg
     7621 ggcggtccca aatggatgga gatagattca ttgacaggaa aaatgagcgg tatgcctcca
     7681 aacaatactc atggtgattt gccgttaaat ttacgagcaa aagatcaatt tggtggtttt
     7741 aaagatacta tgatcgttat tcacatcgaa ccaacgaatt atccgcctcc tgtacccatt
     7801 gctgttttcc cggcgatggg cgatactatc actatattta gaaagccgct tgtttacaga
     7861 ttcatatggc gggcatccat tgatcccgat aacgaggata ctgttaatta tttgctacat
     7921 tttacaggca ataatctgga tactatgatc aaagtcaaaa gcgatacttc actcgacttt
     7981 gatagcgctg ttttgaatga gatgtccgag tattctttga agttatatgc agtagatgac
     8041 gaattcagtt cgatgtcaga ttcatcggtg acctttttta ctactcaagc gtatgcaaat
     8101 cgcaattatg tagatgttcc tacagattat aaactacatc aaaattatcc aaatccgttt
     8161 ggacatatga ccaatatttg ttttcaaatc cctgaacttt ctaatgtaac tattactttt
     8221 tataatacaa ttggccaatt tgtagataga attgtactgg aaaaggttga gcctaaatat
     8281 tatgaggtga aatggtttgc aacggcaaag tctagtgcgg tgtattttgt tagatttgaa
     8341 gcttcttcat ttgtttctcg aaaaaaattt caaaaagtca tgaaaattct tcatgttaaa
     8401 taacaatatg aaattagtta attttgcatt aagtgatgga tagggagaca tcagtgtcat
     8461 gaataaaaaa gctctcatta caggtatcac tggtcaggat ggttcatatc tggcggaact
     8521 cttacttgaa aaaggatacg aagtccatgg tattattcgc cgcgcttcga cttttaatac
     8581 cgatcgcata gatcatttgt ataaagaccc gcatgtcaat ggcgtgcgca tgtttttgca
     8641 ttatggagat atagccgatt cggccaatct gatcaaattg attcatcaaa tcgagccgga
     8701 cgaaatctat catctggcag ctcaatcgca tgttcgcgtt tcctttgata ttcccgaata
     8761 tacgggggat gtgacgggtg taggaactgc gcgcatttta gaggcaatcc gtatgaccgg
     8821 gtgcaaggcg cgtttttacc aagcatcaag ttcggaaatg tacggccttg ttcaagaagt
     8881 accccaaaaa gaaaccactc cgttttatcc gcgaagccct tatggtgcag ccaaagtata
     8941 tgcttattgg ttaacggtca attaccgcga atcgtataat atgttcgcta ccaatggaat
     9001 tttatttaat cacgaatcgc cgcgacgcgg agaaactttt gttacgcgaa aaataacgcg
     9061 cgcggtggcg catattgcag cggggttaca gaataaactt tatttgggta atcttgatgc
     9121 caaacgggat tggggatatg ccaaagaata cgttgaagcg atgtggttga tgcttcagca
     9181 ggaaaaagcg gatgattttg tagttgccac cggtgagacc catacggtac gtgaattttt
     9241 agaagtcgcg tttgggtatg tcggtaagaa ttatcaagat tttgttgata ttgatcctgt
     9301 ctattaccgt ccggcagaag ttgatttgtt gatcggcgat gcatcgaaag ctagacaaat
     9361 attacactgg caaccaaaaa ctacattccg agagttagtg gagttgatgg tagaagcgga
     9421 cattagcctg ttagaacaaa cgcataaaat caatattcgt aaaaaataat gaaaaagaac
     9481 gataagattt ttgttgctgg acataaaggt ttggccggtt cggccattct tcgcaaactg
     9541 attgatcagg gttatactaa tctggtttta agaacccatc aagagcttga tttggaaaac
     9601 caaactgatg tccgagtttt ttttgaaaag gagcgccctg aatacgtttt tcttgcagcg
     9661 gcaaaagttg gcggtattca tgcaaataat gtttatagag ctgattttct cataaaaaat
     9721 ctcaaaattc aaaataatgt tattgagtct gcgtatcggt tcggctccaa aaaattactt
     9781 tttcttggaa gctcctgtat ctatcctaaa ctggctccgc agccgcttaa agaagaatat
     9841 ttgcttacgg gacttttaga gcctaccaat gagccgtatg cgattgcgaa aattgcagga
     9901 ataaaattat gcgaatcgta taaccgtcaa tatggtacgg actttatttc ggctatgcct
     9961 acgaaccttt atggtcctaa tgacaatttt gatttgaata actcccacgt tcttccggca
    10021 ctcataagaa aatttgttga agcacgggac cgtaatgcat cggaggtaac gatatggggc
    10081 acgggtacac ctcttcggga attccttcac gttgatgatc ttgccgatgc gcttattttt
    10141 ttgatgatgc attactcagg aaatgagttt gttaatgtcg ggacggggcg tgaaatttct
    10201 attcttgatc ttgcacattg tattaaacgg ctaacagctt attccggtaa attggaattt
    10261 gattcatcga aaccggatgg cacccctcga aaagttatgg atatttcaaa actgaatgcg
    10321 atgggttggt accctaaaat ttcgttagat acggggttga aacagacgat tgattggtac
    10381 gaaaagcatg tataataatt tgatttcatt cgtagtaaaa aaaatgtaac ttccaaacag
    10441 gaataatgtg gataagtgta tagcttttta tgcatacgca gcgtcggatg ttatattttc
    10501 ttcgggttac cgcagacacg gtaggagtgc tcgtggcggc gttgagctcg atttattttt
    10561 tttcgattcg cgatgcgcgc attacgacca attttgatct ctttcttctt acttctttag
    10621 ccttttcctg gtacttcctt gcacgcatga ccggtttata cgatgagttt cgttcacgta
    10681 acttcagtta tgaactcgtt ggtttgatca aaaatatttt gtttcaaata gtcattgcca
    10741 tcgtagtgat gtttattgca aaaaatcata cgttaccgcg actatttatc gttctttacg
    10801 cagcccattt acttattatc ctatccattg agcggttttt ggttcggagg gttttaaatt
    10861 ttctgcgccg taaaggacgt aatctaaggc acctgctgat tataggagcc ggtgaagtcg
    10921 gtacagcttt caatgagctt attcgatcca attctcattt cggttatgcc gttacgggtt
    10981 ttcttgacga tcagccgcag gtatctctta atggaaaatt acttggtaaa gtcgaagatc
    11041 tggaatcggt attacaaaaa caccccgtcg atgatgttat cgttgctctg ccgacatatg
    11101 cgtctgaaaa aatagagtgg atcatcaaaa tttgtgacca tcatacgcgc cgtgtgcgca
    11161 ttattccgga ttatttccga tttgtttctg ataaatttga agtgcgcatg ttcggaagat
    11221 tcccgctcat ctccgttcgg aaaatcaaaa tggatgaagc gcattggtat atcatcaaac
    11281 gttgttttga tgcgtttttt gcgttggcct ttatggtact tgtattgtct tggttggtgc
    11341 cgattatcgc gattgcgatc aagctcacgt cgcgcggccc tgtgtttttt aaacaggaac
    11401 gctggggacg gcaaaacaaa aaaattatct gttacaaatt tagaacaatg atttctcaaa
    11461 gtcgggatgt ggatgacagc gggaaatacc aacaagcgac ccaagatgat ccgcggataa
    11521 caccattggg aagattttta cgaaaaacaa atttggatga attgccgcaa ttttggaatg
    11581 tgttgatggg acagatgtcc gtcgtcgggc cgcgccctca tcctaccccg ctcaacatgg
    11641 aatccaaaga agtgatcgaa aattatatgc ttcgtcatct ggtaaaaccc ggtattaccg
    11701 gctgggcaca agttaatggt tatcgcggcg aaacccgtga cccggaactt atgcaaaaac
    11761 gtgtgaatca tgatatttgg tatattgaga attggtcgtt ctggctcgat attcaaatca
    11821 tatttttgac cgtatggcaa atgatcaaat acgatacgaa aggatattga tgttggcgct
    11881 tcattctcaa aagtacatag ttgcgacagg attggcggtg ctcgtagccg cttttttaat
    11941 gccctgccca ggatgggcgc aatatacagg gccgtctgtt aacctcccga tggggcactt
    12001 cggatacgat ttgatagagc atcttgagac acggggtgtt gtatcgccat ccattcgtct
    12061 tcgaaccaaa ccgtatgacc gcggaaccgt acttaaaatc ataaatgaag tgcaggacaa
    12121 aatcaaaaca gatgcgaatg ttttgacatc ggccgaacgt agtttatttg aaaagctcaa
    12181 gggcgaattt tatgtagaac taacctctac gcagaacata caaacatcag atgtgcgtga
    12241 gaaacatatt tttgaatatc aaacgcacga agacggattc agttatttgg tgggtgacct
    12301 ggttattgat cagagcgtcg aattgacacg attctccaaa aaaaatgatt cattgggtgg
    12361 tacgataggc ctttccaaag gtagtgggcg tgtccgagga ttgataaaag gcggcattgc
    12421 gttttatgcc gatgcgtcca atacattggt taaaggagat gaacaatcta ccgattttgg
    12481 tagcagttat cgtggcggtg tactcaacta taatccggcc agcgctaatc gttatgcgtt
    12541 acactctgat gcatatatcg ttattgagcc aaaatggttt cgcctgcaag tcggaaaaga
    12601 caatatggcg tgggggccgg gatattatga taatcttttg ctctcgggca atgccccttc
    12661 gtttgaaaat ttgagattgg atgttaccta caaccgattc aaattcagtt atgttcacgg
    12721 ttggttaagg gatgatcgtg atacggtgaa tggacgaaaa gatttaagtg atcggaaata
    12781 tatcgttgcg catcgcgtgg aatggaaagc gttaccttgg ctttttctct ccggaagtga
    12841 aagcgtgatc tacggagggc gtgacgtaga gctttcatac ctgaacccgc tcgtgcctta
    12901 ccatgtcgcc gaacaatatc taggcgacaa agataataat acaatgagtt ttgatgtgct
    12961 tgctttttta cccggacgaa ccaaagtgta cggtgctgtg taccttgatg attttacatc
    13021 ggcacgaaat ccatttacat attggaagca aacctgggcg gttatgggcg gcggttattg
    13081 ggtggaacca atggggttca aggatgccgg tgtgcgcatc gaatattcac gcattgaacc
    13141 ttttgtgtat gcgcataaat ggcgattgct gaattatacg cattttgatg ccggtttggg
    13201 aagccagctc cagcccaatt ccgaccgatg gaatttttta ataagctacc aatactcccg
    13261 tcgtcttcgg gcggaagtcg gtttcaggcg aatacgccac ggagaaggcg atattcagcg
    13321 gtttggatac gaatatggtt ttaccgacga tgattccggt aaacagaaga aacgctttct
    13381 catgggtatc caagaaatta gaaattcggc gttgttcaac gcctcatttg aaacatggtc
    13441 acatcacgcc ttatatctcc agtatgagtt gtctatgatt cgaaacttgg gtaaccgtaa
    13501 gggttttgat gcgacgcaga actatctgcg catcggttat acgcttgatt attaggcacg
    13561 ggacgggtat attcgcgcgg ttttaaaatt caaattttca gaggtaatgt atgtccgtca
    13621 ttcgtctcat aggtgttcca ttggatttgg gtgcagggcg ccgcggaacc gatatgggcc
    13681 cgtccgcttt gcggttggcc ggtttgacgg agcgacttca ggaattaggt cacaccgtta
    13741 aagacaaggg ggatatcgtt accaaaaacc ctgaagtcat tagggtcggc gacgaaaaac
    13801 tcaagtacct caatgaagta acgcgtgccg taacggcgct ggccgatgac gtcgaaaaat
    13861 gcctcgccga taatcatata ccgcttgtac tcggcggcga tcactccatc gctatcggca
    13921 cactggcggg cgtatccggt tattttagaa aaaacaatca gaagatcggt gtgatttgga
    13981 tcgatgcaca cggcgatatg aatacgcccg atacctcgcc ttcaggaaat attcacggca
    14041 tgccgcttgc cgtatcactc ggacacggac atcccaagtt gaccggtgtc ggcggtgaat
    14101 ttgtcaaagt caatcctgag aataccgtac ttgtaggtat tcgtacattg gatgacggag
    14161 aaatcgataa tatccgacgt atgggggtca ccgtttttac gatgcgcgac attgacgaac
    14221 aaggtatgtc caagattata cgacaagcta ttgatatagc cgcgcgagat accgcaggca
    14281 ttcatgtgag ctttgatgcg gacagtatgg atccgagtat tgctccgggt gtcggaacgc
    14341 cggtacccgg cggacttacg taccgtgaaa cccacctcgc gatggaatcc ctgtgcgatt
    14401 ccaaaaaagt aatttctgcg gaaattgttg aaaccaatcc gatacttgat attaaaaaca
    14461 cgacggcaca tgtcggcgtc gaaatgatgg cttcactttt cggtaaacga attatgtaaa
    14521 ttcggccatt gcggccgcga ccggagcagt actgcgaatg atttttcgta tcatcactct
    14581 tctatgctgc gcgcttttcg ttcaagcgca aaatgatttt cgtgtcctcg gcgaaaaaaa
    14641 cggctatgat ccccagacct tacgcgagta tgtcaatctg accgaaaccc gcgccttttt
    14701 agacttggcg ggtgagtgga ccatgtccgt ggaaggagtt tcacgaaaag tcattgttcc
    14761 cagttcttat gattataacg gcaaagttac gtttacgcgt acgttcgttc ccggcgaaga
    14821 ttttcgtaat aagcactttc gttttgtcag cttaggtatc aattttcgcg ccgacatacg
    14881 catcaatggc gaatttgtca ccaatgccaa taacagttat acgcgattgg aagcggactt
    14941 gcgtgaagac ctgatccgta tcggtcagga aaacaaaatt gaaattacgg tggattcgcg
    15001 tgttgatccg tttaccagct atccccgtga aaccagttta ttgcaaccca aattgtacgg
    15061 cggtattacc cgtgaactct ttctcgtagg tcttccaaaa gttgctattg atcaatacac
    15121 cgtaggttat acgttgtcca atgatttgcg caaatgtgat tttgtggtca attggcattt
    15181 gcgtaattac gaatatagtt tgcgccttgt ggacacattg cggttgatca accccaatca
    15241 gccgttgcgc tatattttgg aaatgtttga ggaaaataat ccggagccgg tgtacacaaa
    15301 tcggtttaaa aaatatcgca acgtgtggga cattccgaaa attcgcgagc aaaacgaaga
    15361 agatacggtt tcggttttca attttgttga tcgtcaatta cgtttttcaa tagaaaaacc
    15421 ggtcttttgg aacccgatca cgccgagtcg ttatcgcgtt gtaatgtatt tagtacaggg
    15481 cacgacggtg atcgatcgtg tcgaatacca actcggtata gctcaagccg atgtgcacga
    15541 tcagtatatt tttctcaatg cgcaacccgt gacgatacgc gcggtcgaat attatgaaga
    15601 ctatccgggc gccggcagca ccatgagctg ggcgatgatg gagcaggata ttctcaaaat
    15661 caaacgcctt ggcgctaatg ccgtgagttt tcgtcatcat ccgccgcatc cgtattttgt
    15721 agaactgtgc aatcgttatg gtttgctttt gttttatgaa attccatcct ggcggcttac
    15781 ggcggatatt atgcgtacac cgagttacgt cgaagatatt cgcaaatact atcggcacat
    15841 cgtcgaatat gagcatctca atccgagtat tcttgcctgg ggtgtaggat ttgctcccga
    15901 tccgtcggat ggcgcagcca attattactt tcagcaaatt gtcggtgaaa taaaaaataa
    15961 agatcgccgt cccgttttcg ccgcttcggc ttttggcggc tacgattcgt ttatggacaa
    16021 tgtggatatt gtccagctgg atattcgcct caatagtcct ctgtcttcca atcttttcgt
    16081 ccagcgcagt ctggccaagt ggcccacacg tgcattgatc gcggtgtacg gtgcgcagat
    16141 tttttccaat aatcagaatg gctacagcga cccgatgtcc accaaatttc aggctaaggt
    16201 gttggctgat ttttataaaa agaccggcga gtggaaattg gccggcggcg tagtgcgttc
    16261 gttcaatgat tttgccgtac agcgcccgta cctttatgcc aatccgaatg cgagctcaac
    16321 gatctatacg tccggggttg tgacttacga tcgtcaggag cgctatgcgt ttgaaacatt
    16381 gcgtgccttg tattcggatg atgccttgga accgatcgcc gtcggtgcct acgaacccgt
    16441 atatcccaaa acgtatccga tcacgggact gatattggtc gtcttgttta tcagttttta
    16501 tcggcaaagt cctaaattta aaaacagcgt tcaccgttcg ttgttcaaag tagtaagctt
    16561 ttttggtgac attcgcgaaa accgcgtcat cacactgtgg ccggcgttgt tagtcggctt
    16621 tcttgcgagt ttggcgtatg cggcattgct gtctatcgtt ttctttgaat tgcggcggaa
    16681 ccatttgttt gatgagtacc ttgcgacatt cttgcgcagc aatggtctca aagcctgggt
    16741 ggacggcttg gcatggagtc ctgaactttt tgtgctggag tggacgatga tttacgcttt
    16801 ggtcatcacg gtgatcggac ttctttttat gatatttcaa aaaccctttc aggcgtacgt
    16861 aaatttccag caaagtatta ttgcggcggc gtggagcagt tcgcattttc tgtatctcat
    16921 tccatttgtg gtggtcttcc atcgtctcat gaaaaccgat tttgttttga gcctgggggc
    16981 aacggcggcc gtattgtttt tcttttggca ttggattcgt tttttccgcg cagcgcaaat
    17041 actttttaat gcgagttggt ggcgtacggg atttatttta ggcggggcgc tgatcgtttt
    17101 gtgtacggct ttgggaattt attattcggg atcgcatgat ttttttaatg tgtggaatta
    17161 tatcgaacaa gtcagctcat ctaaaaacta tacaaccgaa tgatgaaaca ggaagaagta
    17221 ctcggtattt tcaaacaaag cggcgcttta ctcgaaggtc attttatcct gacttcgggc
    17281 gcacacagtc ctaattattt tcaatgcgct cgcgttttac agcatcccaa ctgggctcag
    17341 ctgttgtgca gtgaaatagc attgcattat aaagataaaa aaatccatgc cgtggccgca
    17401 ccggcagtag gcgggatcat cgtagcgcat gaggtagccc gcactctgaa tgcacgttgt
    17461 atattttccg aacgtgaaaa cggcaccatg cgttttcggc gcggttttga aatcaaacgc
    17521 gatgaaaacg tgctggttgt cgaggatgtc gtgacgaccg gcggttccct caaagaggtg
    17581 attcaacttt gccgtgatgc ggatgcgaat gtcgtcggcg tcggttatct ggtggatcgc
    17641 agcggcgggc gtgccgagtt tggcgtacct aaattttcct tagtctctgt ggatgtagtc
    17701 aaatatgaac ctgaaacatg tcccctatgc cgggaaggcg cattgccggc gatcaagccc
    17761 ggaagccgtt cgatgaacaa ataagccgaa gaccttctca aaaaattgat ttcttgacta
    17821 cagccccggt ttcagtttaa tttccctaaa cggagatagt gtgttaacag aagcgaacaa
    17881 tgcggtgcgc gtatgcgtac ttggcagcgg aagcaaaggc aatagtacgt ttttgcagta
    17941 cggtgcaact aaagttctga tcgatgccgg gttgagcggt gaagaaacga tggatcgcct
    18001 tacggcggtc gctacgccga tcaacgaact gcaacacatt tttatcacgc acgaacacga
    18061 tgatcatatc aaaggtctga aaaaaatatt gaaaagtttt agcggtatgg tgcattgcaa
    18121 tgcgggaacg tttcgccatc tgagacatcg cattccggag cagacgaggg tgcatgtgtt
    18181 ttccgataca tttgaagccg acgggcttca ggtgcagccg ttttccattt cacacgatgc
    18241 cacggaaccg gtaggttatt cgttccaatg cggccatagg caagtgaccg tcgctacgga
    18301 tatggggtat gcatccgaac tggtgaaagc acgcattcag gattcggata tcttgatttt
    18361 ggaatccaat cacgacgagg gaatgctgat gaaaggacgt tatccatggc cgctgaagca
    18421 gcgtatcgca tcgcgtgtag gtcatctttc caatcgtcag gccggcgaat tgatgcgcga
    18481 tacgatgttc ccaaggctgc aatggcttgt gctggcgcat ctgagtgaac ataataatat
    18541 gccggatctt gcgatggcgt ccatgacaga acatttgcaa actaccgcga tgaaggagca
    18601 aatccaagta catgtcgcgc atcagcatac gatcagcgcc gtgatggagt taaaccattc
    18661 gaatgtaacc gaaacagcac atcatgtctg aaaacactaa aataacgaat acacaggata
    18721 tcaccgatcc ggaacgtctg atcgaaacgg acgataaatt ttcaaaatcg gatcatgttc
    18781 attataaaga aatcctgcat cgttattatc gtcagtcgct tccgtttgat attcaatcct
    18841 ataaaaactt agatgaacaa ccgcccgatg cgcgtattgc gatgaaaaac gatccgtttt
    18901 tccgcgacgt aggaaaatat gttcacatct tggattttag tgaacaggag cgcatcaaat
    18961 ttggccgtga aaacgaagta caacatttga tcgtgttttt gttgtttgaa cgcgacctgg
    19021 atgttctcga agcggttttt ggtaatcctc agttgcccac caaggtattg tatgatttta
    19081 tcaatctgat caaggaacgc gacatcgatc gcgaagatga taaaatcctc aagcttgcac
    19141 aaaaggtcat gaagcggcgc agtcgtcgta tcgtcaaagt tcgtgagata catacggcca
    19201 atacgcagcc gatacaacgt gctaatttga ttacattgtt ttcatacctc attgacgatg
    19261 atgcgcaagt gcgaatggcc gcagaaaatg tcgtctctgc attggatgtg caaacgttga
    19321 tcaatgcgct cgaagatgat tcgctcatac acaccatgcg ggaatccata tccaaaataa
    19381 cggcgatttc gatttttcgc atgctggagt ctgcggttca tcttgaaatt cgtaacctgg
    19441 ctgccggcgg tgatctgggg gcggatcaga cgaatgaaca caatgccgca cgtcggcaac
    19501 tcacacaggt attgcaagac cgcaaacgaa gagagttaga aaagtgcgtg gaagacccga
    19561 ccgatctttt taatgctacg ctgattacct atcttcattt tgataaagat gccgatctga
    19621 gggcgcaggc gttggaaata ttgcagccgg atgatattat tgatctggta tccgatgatt
    19681 cgacgccacg gcatatttca tcgcaagtgt tgccgatgct tgaaaagagc accgatgaaa
    19741 gagtgcgtca acgtaccacg gagattcgtc tcaaagaagc cgaacgatta aataaaaaaa
    19801 tgaaggaaat cgaagtcagc gttaatgctt attttgacgt gatctttcag tctctcaatt
    19861 acagtcgtat caattcgcaa aaagaagctg tcactatgtt gcgcggttca ctgaattatc
    19921 ttcagctttt tgccaaagaa gccaaaaatc tcgaatccgg atcagcaacc gtcgcgcagg
    19981 gcgtgattcg ccaagcgatc gagcattttg aaggttcgat caatgcgctc tatagcgata
    20041 ccaaacgtga gctttttacg gaatttgaag agattcaagg tatggtgcga cacattcttg
    20101 atctgaagag tttcaagttt gaagaagaat ccggtgttga agacgaagtg gatgaagccg
    20161 tactcaataa agctgtgatg atctggcgta atacgatcag tcagtttctt ggccgtatca
    20221 aagaccttga agagatgctt cgcatcaaat ggggtaaatt gatgacggaa aacgaaccga
    20281 aacgtaaaca ggaagaaaac gaaagcgatc tctatgaagc gttcggcgag atcgaaacca
    20341 tgcataaaaa cgaggtggat tgtaaattga aaatcccctg ccgtgagtgc aagcgccgcg
    20401 gttgtgcatc ggaacgattt atgttacagg tggattttct attagaggaa attcatcaag
    20461 ggtttaaaca cgacaaacaa acccatcatg cctaaaccgc ttccgcgttc gttttatgcg
    20521 cggcctacct tagaagtggc tcccgaatta ttaggaaaaa ttttgcatca cgggacgatg
    20581 tcggcgcgta ttgtcgaagt agaagcttat cgtgccggtg atgacccggc gtcgcatgcg
    20641 taccgcggtt tgacgcctag aaataaaata atgtttggcg tgcccggcca tctgtatgtg
    20701 tattttattt acgggatgta ttactgtgcc aatgtcgtat gcgaaaaaga aggtacagcc
    20761 ggtgcatgcc tgatccgggc ggttgaaccg cttgatggcg tagaacgcat gcaagctaac
    20821 cgtgttatgg ctcgcggctt tgctttgagt aacggaccag ggaaactatg tttagcgctg
    20881 ggcattgaca aatcccataa tggaaccgat ctgacgaccg acgatctgta tctttctgat
    20941 gaccgatacc atgcaaaaat aaaaacgtcg ccgcgtatcg gcattcgcgt agggacggag
    21001 aagaaatggc gttattttat tgatggcaat gcatgggttt cgccgtctag aaacaataaa
    21061 taatgaccca cccgcgttcg ctttcgtttc tactagatgt cctgatattg gggatactac
    21121 tatatggtgc gcaatattca cacaaagtcg caaagtacta agtaattttt tagtgcggct
    21181 tgacgatgat aaattttaat acggacacct cgaattatta ctcacgtgcc tattcggtgt
    21241 ccgggatgat cgggtaatat tctggagcca acagaaatac gctgtagaac ccgcttttat
    21301 ttgtcatgcc gttcttctat attcactcca caaaaataat tcattattca tcaggtacac
    21361 gtcggtttta ctatgagcga agcacaacaa aagactgaaa tcccgaaagc ttacgatccc
    21421 aaagcctctg aagacaaatg gtatgcgtat tggctgaagc atgatttttt taaagcccat
    21481 gccgatagcg ctaaaccggt ttttactatt gtcatccctc cgcccaatgt aacgggtgca
    21541 ttgcatatcg ggcatgctta taacaattcc gtgcaagaca tttatacacg cttcaaacgg
    21601 atgcagggat ttgaaacgct gtggaccccg ggcacggacc atgccggcat agcaacgcag
    21661 accgtcgtcg aaaaacaatt agcgaaagaa gggacttcaa gaaaaacatt aggccgcgaa
    21721 gcctttgtca atgaagtgtg gaagcataaa gagacgtacc gcgcgcggat cgtcggacaa
    21781 ttgcaaaaaa tgggttgttc ttgcgattgg gagcgcgaat ggtttacgat ggatgaaaaa
    21841 ctatcagagg ccgtcaaaga agtattcata catttttaca ataaaggctg gatctataag
    21901 ggtcagtata tcgtcaattg gtgtccgtat tcgcaatcgg ctatttccga tgaagaagtg
    21961 gaatacaagg agatcaacgg aaagatgtat tatttccgtt atcctgtaaa agacagcgcc
    22021 gaacatatcg tcattgccac gacacgcccc gagacgatgc tgggtgatac ggccgtcgtc
    22081 gtcaatccgg atgatgcgcg ttacaaacac ctgatcggaa aaaaagtgat acttccgttg
    22141 gtgggacgtg aaataccgat catcgccgac gactatgtgg ataaagaatt cggtaccggc
    22201 gcgatgaaag taacgccttc ccatgatccg aatgactttg tactcggaaa acgccatcag
    22261 ctgcagtttc ttaatatcat gcatgccgat gcgaccatga atcaaaacgt gcctgaaaaa
    22321 tatcgcggca tggagcgttt tgccgcacgc aaagctgtgg ttgccgattt agaatcggca
    22381 ggattggtcg aaaaaattca ggatcactta aatcgcgtcg gttattcgca gcgcggaaat
    22441 gtgcccatcg aaccgttggt ttcggaacaa tggtttctga aaatggacga attggctaaa
    22501 cctgcgctgg aggccgttct taaaggtgat atacggtttc atccggagaa gtggattaaa
    22561 acgtatgagc attggatgac ctccattcgc gactggtgta tttctcgtca gctttggtgg
    22621 ggtcaccgga taccggcatt ttacgcgccg gacggaagtg tcgccgtagc acgtaatgcc
    22681 gatgaagcct tggcgatact aaagaaaaaa aatgcgcagc taacggtttc cgatttacgt
    22741 caggacgaag atgtactgga tacatggttt tcttcgtggt tggttccttt ttcatctatg
    22801 ggttggccgc atcaaacaaa agatctggaa aaattttatc cgacgagcct tttggtgacc
    22861 ggtccggata ttatcttttt ctgggttgca cgtatgatta tggccgggtt ggagtttacc
    22921 ggtaaagttc cgttttgtca ggtttatttc aatggtatcg tgcgtgacga actcggccgc
    22981 aagatgagca aatcgctcgg taacggcatt gatcccatcg ccgtgattga taaatattcg
    23041 gcggatgcgt tgcgatttac gctcgttgat ctcagtgccg aaggtcagga tatcaatctc
    23101 gcggaacgcc aatttgaact cggccgcaat ttttccaata aagtatggaa tgcgtttcgt
    23161 tttttggcga actatcagga gcagctacct agcgctaaac cgcaactgcc gccttcgaac
    23221 gcgatcactg aactcgccga tcggtggata ctgagccgat atcatcgtgc cgttgaaggt
    23281 attactaccg gactcgaaaa ttacaaactg cacgaagcga tggattcggt ctatcgtttt
    23341 ttctgggcgg attattgcga ttggtatctt gagctgatca aagatcgtat gtacaatgcc
    23401 actccggaga tcaaagaaaa aacgttatcc acggcgttgt atattctcga cggtgtattg
    23461 cggttgatgc atccgatcgt gccttttgtg accgaggaaa tatggcaacg tctgatgccg
    23521 cgcaccgacg gtgaaagtat tatgatccaa ccgtggccgc agtgcgaagc gtcctacatc
    23581 agcgtggaag ctgaaaaaga tctggagcaa ttgcgggata cgattactac gatccgtaat
    23641 attcgttcag agatgaatat cgcaccatcc aaaaaaacag atctcgttct gcgttgtgtt
    23701 acacctcagg atgcggcgct gtacaatgct aatacatcgg cacttgcggg acttgcgcgg
    23761 gtagaaaaaa tcacggcggc cgtcggagcg gataaacccc gcatcgcagc gagtgcagtt
    23821 gtgaatggta cggaagtcta tttaccgtta gagggtgtga ttgatcttga agtggagcgt
    23881 acacgtttac aaaaagagat tgaccgcatt tcaggtcaga tcggtggtat cgaacaaaaa
    23941 ttaggcaatg aaggttttgt ggccaaagcg ccgccacagg ttcttgaaaa agagcgcgct
    24001 aaattggata attttcgcgc gaccgtagaa aaacttcgat ccaatctttc cgctttacaa
    24061 aatttgtaaa ccttttccaa tactaccgcc ggtgcacagc cggcggttat ttattttatg
    24121 acggcttgga ctgaacgact tcgggatgcg aagccactgc tcttggatgg tgcgacgggt
    24181 tctgaattgg aacgtcgcgg cgttccatgt gttttaccgt tgtggtccgc cgtggcactc
    24241 cttcaaaatg agtacacatc cgttttacga gctattcacg cggagtacat acagcgcggt
    24301 gcacagatca tcacagcaaa tacgtttcgc ctaaatcgcc aaacgctcat gacggagcaa
    24361 ctccataatg attcgagaag gcttatctgc aaagcggtag atacggttcg cgaagcgatc
    24421 gaaagtgtag cccgggatcg tacggttttt atcgccggat cgatcgggcc ggtggaggat
    24481 tgttatcatc cggaacgcgc accggcggat gatgttttac gcgatgacca tcatcggcgt
    24541 attgatgatc tgtatgcagc gcggccggat ctgattttga tcgagacgat gaataatatg
    24601 aatgaagctt ccattgcaac ggaatatgcc gttcagaccg gattacccgt tgccgtcagc
    24661 tttattgcgc gcaatgatac gcaattgctc agcggtgaat ctttagaaaa agccatacgg
    24721 cgtatcaaaa cgtataatcc cgctgcactc atgctcaact gcatgcacgt agaaatcatg
    24781 gaacatgcgc tggatgtatt gaaagatacg gccggtgata tcccgatcgg cgcttatgtc
    24841 aatatatttc cggaacaatc taagcctgcg atgacacccg aggattttgc ttcgcgtatg
    24901 gaagcgtggg tatcggagta caaattaaaa ataatcggcg gttgttgtgg tacgacgccg
    24961 cgtcatatcg aaaaacttgc cgaacgtatc ggcgtgaact agttcaaaac cgactcatgg
    25021 tatggtgtaa acagaacatg aatacaggct gtatttcaaa acggtttcaa ttcgcgtcct
    25081 ctatgctgtt gtggatgaac gttacttttt tagcggcgca gacagaacgt ttgtatgtag
    25141 agcgaatttc cgttgaacaa ggcttatcgc aaaccaccat tttcgggatg atgcaggatc
    25201 gtcgcggttt tatgtggttc ggtacggagg acgggctcaa ccgttatgat gggtatcaat
    25261 ttcgcatttt caaaaacgat ccgtttgatt ccacctcgtt atccaataat tacatcaaca
    25321 ctctctacga agaccgtcgt cggttcattt gggtgatgac tttggaaggc gtaaatcgct
    25381 acgatccgcg cgatgaaaaa tttttacgat tcaagcgtag cgacgatacg acgaccatcg
    25441 cggggtatcg tattgaaaat atggccgaag acagcgtagg gcacctatgg tgtacaacgg
    25501 atcgcggcat gctcctgcgc gtagatgaaa acgggaagat acaggaacgg tggaatccgc
    25561 gttctgccga tgacatgtcg tcggttcaaa atttgcaaat tgaaaacgga aaccgttttt
    25621 ttctttcgac caattcgggt ttatacattt gtgatcctac ccggcgccac gtcgtacgtt
    25681 atcatcgtga agctgccggt gactatcgtt tgtcggaaaa cagtgtgcga tcggtggtaa
    25741 aagaccgacg gggttggtat tacgttgcga cgtacggtgg tggtctatat atgtatgatg
    25801 aagtttggcg ccttcagaat aattttaaaa aagcagacgc ctcgaaacac ggccttaccg
    25861 aagatgattt gcagacgtta tactgtgatc gtgcagggcg tatctgggtc ggcagcgaag
    25921 gcggaggatt atttcttttt tcgccggaac aaaacaagtt tttgcagttc aaaacggagt
    25981 caggcgatgc acacagccta agcaataacc tgatattgcg tttgacagaa gatcgtaccg
    26041 gtgtaatttg gatcgggcat cgtgacgggg gaattagcaa atacgatccg caacgcatac
    26101 gttttgtgtc gtaccgacat gatccggatc ataccaatag cctaagccac aatagtgtgt
    26161 attctatttt tgaagatccg gctgattcgg gaaaagtact ttggatcggt accaatggcg
    26221 gcgggcttaa tcgctgggat cgcataacac agacctttcg ttcgtatgtc aatacgccgg
    26281 gagttcgcca tacgatcggt gataattccg ttcgtttttt gttcaaagac agtgaaggtg
    26341 ttttttgggt aggaaccaat aaatccggtt tgcaacggat ggatacggat cgcggcttgt
    26401 ttgaaccttc aaccaatccg aatatcaaat ttattcgtta tgtgatcgaa gaccgcaaag
    26461 ataaaaatat attctgggta ggtactaata ataacggtgt gtttcgcatc gaaaaatcgg
    26521 gaaaaatttt gcaacagtat agcccgcaaa ccaagccggg tttgagtttt aacattattc
    26581 gcgggctcta tcaggacagc gacggtaaag tctgggtggc gacgcaaggg ggcgggttaa
    26641 attgtatatc agcggacggg caatctgtaa cggtttataa ttatgatccg actaatccgc
    26701 aaagcctcag caatagcatc gtactttgca tattgccgga tacgacgcaa aatatatttt
    26761 ggctgggaac agccggcggt ttgaaccggt tcgattcgcg cacaggtatt acacaacatt
    26821 ttcgtgaacg cgacggcctg gccaacgatg tggtgtatgg cgtactccgt gacgaaaagg
    26881 gttttctatg gatgagtacc aatggcgggc tgtccaagtt tgatccgaaa acctcggttt
    26941 ttcgtaatta cgataccaaa gacggtctgc agagtaatga atttaatgcc ggtgcatttt
    27001 ttaaaaatca aaacggtgaa atgttttttg gcgggataca agggttcaac gtttttcatc
    27061 ctgaccgcat tacggataat acgatgccgg tatctgttgt gcttacgcag tttcagatag
    27121 ccaatcaacc ggtcgcggtt gatccgaacg gcgtgctaaa acaatccgtc acggaatcgc
    27181 agcagatcgt attgccgtac gcgggtaacg catttgcttt tgaattttca gcccttatgt
    27241 atcatgcgcc ggagcaacat cagtatgctt accgactgga agggtttgac aaaacatggt
    27301 tgtacagcgg cacccgccgg tatgtgagtt acaccaatct tgatccgggt gaatatatct
    27361 ttcgagtaaa agcatccaat catgacggtg tatggaatga agacggtgcg tcgatgatga
    27421 tcatcattac accgccattt tggcaaacgt ggtggtttcg cctgctgatg ttagggtttg
    27481 ccggtttgat cgtgtggggt atgcttaagc ttcgatcata ttatgcagag tatcgcaaga
    27541 cacattatgt cagtcattat aagttgcttg aaaaaatcgg cgaaggtgga atgggtgtcg
    27601 tgtacaaagc gagggaccgc gtatcgggaa agacggtagc tctgaagtta cttcacgaaa
    27661 gtattgcgga taccaaagac ggattgcaac gttttttaaa agaagcggaa gtgggtaaat
    27721 tgatacacca tcctaatgtc gtgaaaatat acggagcggg atcgagcgaa acgcgtcggt
    27781 tcatcacgat ggaaatggtg gaaggcaaga cactgaagca aacgatacgc gagcggggag
    27841 tttttacgaa agacgacacc aagaaaatta ttattcagtt agttgaaggt ttagcgcaaa
    27901 ttcatgtaca cggtgtggtg catcgtgatt tgaaatcgga taatatcatt atcaaaccgg
    27961 acggaagggt agtgatcatg gatttcggat tagcaaggat caagggaatg acatcggtag
    28021 cggcgagaga ccagcttgtg ggtacactgg cctatatgag cccggagcaa acgatcggca
    28081 aagaagtaga tcatcgctcc gatatctaca gccttggtgt gataacctat gaaatgctgt
    28141 acggtgaaat gccttttata gccaataatg aaatggaatt gatcttggcc atccataatg
    28201 aaacgccgaa acatattgat gcaaacaatg tagatgcgtt gcatcagtgt ataaaaatat
    28261 gtctgtccaa agatccgatt gatcgttatc cggatacaac tacacttaga aaatttttag
    28321 acacggtgtg agttactttt gatgttccga aaattattac atggctactt atttcttctt
    28381 ctaacctgct ttcgcttgtc ttctgcgcaa taccaattgc cttttgttca ctacacggcg
    28441 gataatcaga tcaatcctct tcccggtgta gctgtctatg atgtttatca ggaccgttta
    28501 aattttctct ggataagtat tttttcagca ggattagtgc ggtatgacgg acaccgcatg
    28561 gaggtatata ccacgcgcga cggattaaga tcgctcaatt ttacaaccac tatcgaggac
    28621 acagacggac gtttgtgggt tatgaccgat ggaggtcttt ccgtttcaga acaacctctg
    28681 aaaacctatg gtgcgcatcg cgttcgattt attgatcgca tcggtgatca gccattggcc
    28741 aaaacttcga tcagtcagat tgcgatgaaa gttttagcgc aagatactgc aggccgaatt
    28801 taccttggca caaccggcgc gggtatttta agatattcac gggtaaaacc tgatttctgg
    28861 agctgtgata cgatcaccac gcaaaatagc cattcatcgc gcaacgcttc cgtgtattct
    28921 atgatggtgg ataaaaaagg cttagtgtgg attagtaccg acgacggcgt tgctatttta
    28981 aagccgggcg gtcatcgcat agaatatttg gaacgttcga ttatacctcc ggttagagtt
    29041 caaacatttt ttcaaacgaa tgatgaaact ttattggccg gttgcgaaga tggttcgatc
    29101 tatcaatggg aaaaagaggc tcatgggttt cgtaagatgg atcatacttt gaccagtccg
    29161 gtatatgaca ttacgcaaga tcgtcgcggc gatttatgga tttgttcgga tggcgccggt
    29221 gtgttgcgct ggagaaaagg gaactcaccg gaacacatca cacgccgtca ggggttaatt
    29281 gctgaaagtg tacgatttgt aatggaggac cgggaaggta cgttgtggtt ttctcaggtt
    29341 ggaggattat ccaaactgag agcaaattat aaggcatttc aaagtatttc ggcggatgcc
    29401 acgttggaaa caggcgtcgt gttgcctgat ccgaatgtca ccggcatttc taccgaagca
    29461 tggaccggcg ataaacctct cctttggatg ggattaggta aaggttttgc ggtattgacc
    29521 catgatggaa aaatatcgca tttcgatgca aaaaaaggtt tatcaagtaa tacggtttac
    29581 gatgtattgg tggatgaaaa aaaacgactt tgggtaggca cgttcgcagg acttaacagt
    29641 tttacattct acggtacacc gccagttact ttaggggcca catcttcgcg acgagttcaa
    29701 ttgaacaatc aaccggcagt tatggatttt catgatattg gtattacggg cgtagtctcg
    29761 cgttatgcga tgagggaaaa cccggaggca caacgtacgg ttgaaagtct ttggttcaac
    29821 agcattcgcc gactcagtgt ctatgtcggt aattcgtggt atatttttga tgaagcctcc
    29881 ggtttaccat ccagtatcat ttatagcatt gcgtgggata gtgcgggttg gatgtatgtc
    29941 ggaacgggcg atcaaggaat ataccgcagc cgtttttctt taacgtcgtc agagttaaga
    30001 aaacaattcg aacgatccgg taaaggttta taccaacgcg aaatcaccac gccgtgtttt
    30061 gaaaaatact ggaatcagga gaacggcgct ccgtttgatg atccgcaatc acttttgttt
    30121 gttggtgatc gcttgtgggt aggttctgcg tcgcgcgggc ttgtcgtgat caatccacaa
    30181 acacgtaccc ttattcatca aatgaattcc gatgccggtt ttcgaagccc gggcattatc
    30241 aatttaattt tttctccggt tttcggcacc gtttggtgcg gctcctccaa cggtatatac
    30301 gaaatagatc cgataagtta taagttgctt cgcagcgttt ttcgcgacga tggtttaata
    30361 agtagcgagg taaattggtt aacctccacg gcttttgata gcgatgggaa attatatatt
    30421 ggtacgccta aaggtatgac gatctatgat cccaaattag accgtccaga tcttacttcg
    30481 ccgcaaccgt ctattacttc ggctacattt catcagtcat taggcggaga aaatgagttg
    30541 ttaactgaat tttccgcttt gagttttacc aatgaaaaag cggtgcaata taaagtacgg
    30601 ttggtagggt atgaaagcga ttggcatgcg gtatcggaac cgagaacacg atatacgaat
    30661 ttgccggctt ggtttaccga ggcgcgttat cgtttcgaag tgcttgcttc caacgctcat
    30721 ggcgtttggt ctgaaatgcc tgctgtttat gaatttaatg tcatgccggt atggtggctg
    30781 cgctggtggg catttgggat ttatattgcg ttgttcagtg cgttggtttt gggtgcgcga
    30841 tggtttgtta ttaattggcg atccgtgctg atgcccaaaa cgcggtacat tgcgcattac
    30901 aagattttag agaaaatcgg ccgcggcggt atggggatgg tgtataaagc gcgggatacg
    30961 ctaaataaac gaacggtggc cattaaaatt ctgcatgaag aatttgaaca gacggaggat
    31021 ggtatcaaac gttttatcaa agaagccgaa ataggacgta aactaaagca tcctcatatt
    31081 gtcgaaattt atgatgccgg gcgatttgat aaaacgcgat atatcacaat ggagtttatc
    31141 gaaggcgtta cgctaaaaaa ttttttacga gtgcacggac ctttgatgcc ggcggatgtc
    31201 gtacgaatta tccgtccggt cgcacaagct ttgcgaacta ttcacgccca tgatgtgata
    31261 catcgcgacc tcaagtcgga taatgtgatg atacgtaacg acggggttgt taaaatcatg
    31321 gattttggtt tggcccgcac caaaggtatg acgaccgtcg tacagcgcga tcagcttgtc
    31381 ggcacgcttg catacatgag tccggagcag agcctcggta aaccggtgga tgcgcgaagc
    31441 gatatttatg cgttgggtgt aatcatgttt gaaatggtgg aagggtgttt accgtttaac
    31501 ggtcaacacg agatggaatt gatttatgcc attcacaatg aaacgccgga gtttcacaat
    31561 acgaatattc ctgaagcttt gcgtcaattt atattgcgtt gcctggaaaa agaacctgag
    31621 cgccgattag ccaatatgga tgaattggaa gaagtattga ataagatcgc tgtataaatc
    31681 ccttgcgaag cgggctattt cttttaaatt aaaatcataa cacttacggt tcctcacggg
    31741 tatatgatca aaatttatga aagcctgcag gatttgcagg tcaaaataga cgaaacgttc
    31801 aaagagctat cgcagggatt taataaagtc aacggcgcga tcacgcaaaa aaaccttccc
    31861 gccgccgaat ccgcactcaa tgatcttttt aaagcgttta tgtcacgtac ccgtgcgatc
    31921 aacgcccagt cggcgtcggt cggcgcggac atcaaaaaaa tagaatccca gtgcgctgaa
    31981 atatccgccg aaaaagaaca attcaaagcg ttgtataatt ccggtattgc gatgacaacg
    32041 caaggcgaat tgcagggact gatcacattt gcgatgaatc agatgactta tcatcttcgc
    32101 gcggagcgag gttttctgat tttggtggat gatcacggtg agcgcgaata ttttgtcagt
    32161 aaaaattttg acggacaaaa tattgacgat ccggccgccg aagtgagcac cagcgtaatt
    32221 caaaaaacat tggatgcgtt acaaccgatc aaggtggacg acgatacact atccgagagc
    32281 atcatgaaac aaggtagttt tgtgcgtctc gggcttcgtg ccgtgatgtc agtgccgatc
    32341 attttcagaa aaaaattact cggtgtggtt tatcttgacc ggcgggaaga aaccaaaggt
    32401 ttttcggata acgacttggc ttttctgatg gcattttccc ggcagattgc ttttcgggtt
    32461 aatgaactca aggaacaaca acatgttcat accgaatacg aacgtcgtga tcgtaatcgc
    32521 ttacaatcgt tgcgagaaaa atataatttt tcagaaatca tcggcaaaag cgaacggttg
    32581 gtcaaagtac ttgaattggc cggtaaagta gcatcctccg aggttactgt tttgatccaa
    32641 ggcgagagcg gtacgggtaa agaattgatc gcccgtgcca ttcattttaa cagcgaccgg
    32701 tacgaacata aattcatagc tatcaattgt ggtgcgattc cttcggagct tctcgaatcg
    32761 gaactattcg gatacgaatc gggtgcattt accggtgccg tcaaaaccaa aattggaaaa
    32821 tttgaagcgg cgcatcgcgg aaccttgttt ttggatgaag tggccgagtt gtctatgaat
    32881 cttcaggcca aaattttgcg tgttctgcaa accaaagaac tcgaacgtct tggcagcacg
    32941 gaaacgcgca aaatcgacct gcgcatcata tcggccagca ataaaaatct ccagcagatg
    33001 gtgcgggaag ggaaatttcg tgaagatttg tattaccgtc tcaaagtcgt agagatcgaa
    33061 ttaccggcgt tacgcgaacg gaaggaagat attcatcttc tagttagttt ttttgttcag
    33121 aaatacggcg aacagcggat tcagtcggtc agcgaagatg cgatggatat tttagaaaac
    33181 tacgcatggc acggcaatat ccgtgaattg gaaaatgtca ttcaacgtgc gatcattctt
    33241 tcgtccagca cgcagatcga agtgcaggat ttacctccgg agattgtttc caaaacggaa
    33301 agcggatata aggtagacaa agatttgtcg ctcgaagacg cggaaacgga ttttcggcga
    33361 tggttcgtgt tacgcgcact gcggaaaagt aacaacaata aatccaaagc cgctgaaatg
    33421 cttggcatca accgcaccca cttttttcgt atgctcaatc agctgggcat atccgactca
    33481 gaatagctct gttatagctc tctctttccc atcccaaact ccattcctga aatcgcgtag
    33541 catttttgcg accatttaca catttcggtc tgtcgtatta ttgtaacaca ttttacgatt
    33601 taggaccgcg tgagattgat ttttttaatg ttaatttttg gcacagcaat tgatattcct
    33661 ataataccat tatcagggat ttcatagtga aaaaaaaggt tctcattttt aatcaagaca
    33721 ttgatgaatt atcggtcata cgccaggtat tggttcggga agggtatgaa gcgataacgg
    33781 catccaattg ggatacggcg actaaacttt ttgctagtct tgagattgac tacgtgctaa
    33841 tgaacggcaa atccgacgat atacagcgtg ttttgcaaag tgcattaaag gtgtgattaa
    33901 agtattcgta ctgcagcttt aataaagttg ctcgtgcgtg aggaccgcgc ggttctattc
    33961 agccgcgcgg gtttttgaaa aatatgtttg taatcataga caagcgtaaa gcaacaagtt
    34021 aaatttcgat cgtaagtaat cattaaaatc aatgggagga ttttatgaaa catctactga
    34081 cgttcacttg tattttggta ttggttgtgt tgacttcgtg cggtggtgat gacgcggcta
    34141 aaccggaaga atttaaaaca caagaagaag ccttgacagc gttgaatgcg aataaaacca
    34201 acatgcttct ag
//
LOCUS       DAOQGF010000027        33221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_598_length_33221_cov_6.650877, whole genome shotgun sequence.
ACCESSION   DAOQGF010000027 DAOQGF010000000
VERSION     DAOQGF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_33221_cov_6.650877"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>1308
                     /locus_tag="PK937_05745"
     CDS             131..>1308
                     /locus_tag="PK937_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH32106.1"
                     /translation="MLPLLTNEELSQLPPDGGSEYNRLIFEKSPYLLQHARNPVKWWS
                     WCDEAFETARAEDKPVFLSIGYATCHWCHVMEHESFEDAEIASAMNALFICVKVDREE
                     RPDIDHVYMAVTQAMTGHGGWPMTVLMTWDKKPFFTGTYFPKRSRHGRIGMHDLIKAI
                     NDAWLHRKDDVLTNARQIVESVSRAEASEPKPLRTAILSEAVEMFKNQFDQRYAGFGN
                     APKFPTPHNISFLLRYYHTTHDADVLQMVVRTLDAMRWGGIYDHIGFGFHRYSTDARW
                     FLPHFEKMLYDQALTAIAYTEAYQITGYARFKETAQNILEYVLRDMEAPSGGFYSAED
                     ADSDGEEGKFYLWSFSEIAAILGTIDAELFAKVYNLDREGNYYDEATREPNGSNIIFL
                     T"
     assembly_gap    1309..1408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1409..2047
                     /locus_tag="PK937_05750"
     CDS             <1409..2047
                     /locus_tag="PK937_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH32107.1"
                     /translation="HLDDYAFWIWGLIELVETTGDVQFLKEARRLTTIVVDEFEDPDN
                     GGFYFTSQNGEPLIRRTKELYDGAIPSGNSVMLSNLYRMSRLLHEPALAGKAERIGLS
                     FADSIHRMPMAFAQFLSGWDRLQQAPMEIIITGTKDNATFQVMSTLAHHAYHANKTLI
                     IKSSDVWDDLSPQYKNYPINSDYAKAYVCRDFHCEAPVSDKTDLQNKLRNAP"
     gene            complement(2114..3187)
                     /locus_tag="PK937_05755"
     CDS             complement(2114..3187)
                     /locus_tag="PK937_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32108.1"
                     /translation="MPDSREIQTFDFGENDYAISASILQDKGMIIGGSTNSIRDNRDM
                     LVMRIDENNQKVWVKSIGGKTTDRCFNVIELDDHSFIAVGSIQVSSGNLDMLVLRLSK
                     TGEIIWSRNFGGRYNEDGRAVVQADDGSVIVVGTTASFGAGSNDVFLVKITKEGQLFW
                     QRTFGNTSIDVGNDIKKLENGDFIIAGYSSNGIYPFHTLLIRVSSEGDELWKKIYTMG
                     VSSDGRSVSILPDGGYIVSGNVRLSPYSGAISTMILRTDADGNVMWHNSFLGSTETNH
                     GIILKDNIYAVAGQITTRQTNAFVMTVDAISGAQIDFKMYGTSGYSSANAILKKGSDF
                     FVVGWIETDRIKKIDVFIEKINF"
     gene            complement(3267..4403)
                     /locus_tag="PK937_05760"
     CDS             complement(3267..4403)
                     /locus_tag="PK937_05760"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32109.1"
                     /translation="MRFLKLSFLIAVIACENWPENKSQEFFDFGKEEYGVSAIPYSDG
                     GMIIGGTTTSIRGNQDMLLMRLDDSHQKLWVKAIGGNAIDRCHDVFELSDHSVVAVGS
                     VQKSVGNNDMLIVRLSKYGEIIWSKNIGGIYNEEARAVVQANDGNIIVAGTTASFGAG
                     SNDVFLVKMNLAGALLWQKTYGDQKVSGAYDIQLTKDKGFIISGFMANGITPYDAMLI
                     KISESGEEQWTRTYPKSGKANAKRVIELSDQSFLVTGIAQYSDVPGSNSSFVFHADAT
                     GNIIWYQNYNIFVNNESFDMVVLNEQNAVVTGFIDGANTQLYALTVNLSDGIPNGFKR
                     YLDGATSWGYSIIKTENKFWAIGGVIDSDNRTTDVAVIEITLPL"
     gene            complement(4412..6709)
                     /locus_tag="PK937_05765"
     CDS             complement(4412..6709)
                     /locus_tag="PK937_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH32110.1"
                     /translation="MIYRLSLSGLFLLVGIFSLSAQVTFTLDGTVKDLYTGRPMPSVN
                     VYVQEIQKGTLTDTAGYFAIKNLKKGYYTLVLTRVGYKRVTRKVQLFKDINFPIKMEE
                     QAIVFEAQEITPGVTELSIDEGASSTVSTQEIVNSVQVFSKDVYRALQTVPGISNSEW
                     SSKPHVKGGNPDETAVIIDNLEIREPFHLEEIDGPYSVISSDLVKSMKVLTGGFAPKY
                     GDRMSGLLFINTIDRIEDDSMKISVDFSNATASLNQRINDRLTVFASGRKSYIFLLEN
                     AIDNNFPTTIWDGWVKADYKVDPKNRITVNYLMLNDNIQYKESDSSEFFFSTKTNYYL
                     WLNWLNTVSTDRYSTTTLGFQDLTKKSDFLFNSSYTDDNQDNRTTKLLTLKHDHSWRF
                     NRQNTIEFGAEFNQFWSEYYFREFRLNPTETTPILVSQDIIFVDSKFSGYTGAAYFQN
                     SYKINDKMTVLGGLRATGQSYANAIQLAPRAAMSYDFNSDLTLRLAYGWYYQPDNFQK
                     LRSYANQHVPNITPEKSIHYIASGIYKYDDNTNITLDAYYKDYPKLFDDFTFDFSNRI
                     EGVGLVDKPYQTVSGFATGFDLLARHRYGNSNLFSLAYSLSTSHIRNERKLETYRDLD
                     RTHSLLLSNISNLPSRMALSSSFRIRSGDPFTPSTVRILGDGTVENSKIYYTTQTKNS
                     GRLPIFYTLDLRLEKRWTGQQMHWVTYVSVVNVTDHKNVRQRSWKRHINPDGTLAPFT
                     QEDQLYFPRLFMLGVSAEINLPRTR"
     gene            complement(6745..8385)
                     /locus_tag="PK937_05770"
     CDS             complement(6745..8385)
                     /locus_tag="PK937_05770"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNH32111.1"
                     /translation="MPVIGSPAGQWNPEKYQKNRSFMERLLNALRQDGEAIKRGGGQK
                     NIDKQHEKGRLTARERIVKLLDPNAHFMELGLFAAYEMYTDFGGCPSAGCVSGIGLIH
                     GKETVIVAHDATVKAGAHFEMTVKKTLRAQEIAMKNFLPIVYLVDSAGVFLPLQDQVF
                     PDENHFGRIFYNNARMSAMGITQVAAVMGPCVAGGAYLPVMCDKFIMVQGASLFLAGP
                     ALVKAAIGQVIDAETLGGATTHNAISGTADYHEPNDGAALDRIRDILSKIAERPKANF
                     NKIAAVAPQWAMDEIAGILPEGLGQYDMREIIARLIDGSDFNEYKATYGKTVVCGTAR
                     VGGYAVGFVANQKLVQRTVKGEMQMGGVMYNEAADKAARFVMNCNQDGIPLIFMHDVN
                     GFMVGRDAEYAGIAKDGAKLVNAVANSVVPKITIVIGGSYGAGNYAMCGKAYDPRFIF
                     AWPTANISVMGGAQAASTITEIRLAGKQVSEEEKKKLYEEIRSNYEKQGNPRYAAARL
                     WVDAIIEPTETRDTLIRCLDAASNNPNVPPPNFGVLQV"
     gene            complement(8417..10126)
                     /locus_tag="PK937_05775"
     CDS             complement(8417..10126)
                     /locus_tag="PK937_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32112.1"
                     /translation="MPKSFFLVALVVVSAGCNPFNQEQITTLSCYEGQPAFSPNGDVM
                     AFVTTQTGDRHIWLYDLGERITIPVTVNEGVDEHPSFAPDGERIIFTSRLNGQNDLWI
                     VSPNDELIQVTKTDSVDEIFPVYSPDGMYIWYIAISKSAADGGYAIHRTEARNFSKSE
                     VIYRSRGPLEKITIAKDKIVFGEKNEASWVLMRLDPATGSVARWSDGYRDARHAALSP
                     DGKHLFFSSDTNGYAQIFFTRADTFNPVLISGESKHLDFPVWTPDGHAMAYQAETSYD
                     IRRIEVKTQMEQMLIETNGDDRSPVMLADGKTLLYVSNAEGRYALYARNLSANKAIRI
                     AYWPDADVTECDLRNDGTAAVVSVVRGGLSTLYEMPITQANLIEEYAAGKSAYALFQD
                     SVHRYAAHYAIDGKAITYVSLKGGSPDIWVYDTATKKEKQLTIDMRTEANPVFSNDGA
                     YIYFDADWAGRWSIWRVPVSGGMPSPVTRDKTPYGSDREPSWSPDGQWLAITRTWYDD
                     ADIWLYKTDEGEKTSRTLSKDNNHQERRGRWTHDGKYMIYESGGNTDIWMQSVAKLLE
                     ATK"
     gene            complement(10218..11252)
                     /gene="tsaD"
                     /locus_tag="PK937_05780"
     CDS             complement(10218..11252)
                     /gene="tsaD"
                     /locus_tag="PK937_05780"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNH32113.1"
                     /translation="MIASNDDLITLAIETSCDDTSAAVLRGSSVLSNIVSSQIDGLRF
                     GGIVPELASRAHQANIVPVIRAALSAAYITPDDIGLIAVTKGPGLIGSLLVGVNFAKA
                     MAFGLQKPLVGVNHIEAHVLASFLEKDKPVFPFIALIVSGGHTLLVEAQNPGQYRILG
                     ETVDDAAGECFDKTARLLGLTPVEGALMAGPVIDRLASQGNPKAIAFPRPMLQSGDLN
                     FSFSGLKTSVLHFVQKQNQPLAQNLLADACASFQEAAVDVLVGKTRAALKTTGICQCV
                     LSGGVAANSRLRARLTEVCAQDGVQLFMPARQYCTDNAAMIGITGLMHYRMGRTDNLG
                     LSPKASLRIA"
     gene            complement(11233..12171)
                     /locus_tag="PK937_05785"
     CDS             complement(11233..12171)
                     /locus_tag="PK937_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32114.1"
                     /translation="MRLLKSHRIFFLSLCIAIFLWLYVRLGATYQMEIHVPFQVTNIK
                     DGYGVVSDIPEAVDILFEGDGKSLLGMRFLNPPRFVLDMAQHKDKSRFILSEHLHEIV
                     YPSKAPVHAIAVQWPDSVIVRIDRMIARRVPVVADVQITCEAGFILVGGMQLMPDSVT
                     VTMPWSYRDSAVVIGTASHRWENLNSDVRTELRLTAHPDRRINVLQSTTSLFLDVQPL
                     GEIVLDNLPVRLINVPPFTTVAVQPSTFSVRVRGGVDYLSTLPKESVVGEIDYQKEYN
                     SVQPRLDIRVPADLTWTQVTPSRFNLVRIEDDSLER"
     gene            complement(12178..13512)
                     /gene="dgt"
                     /locus_tag="PK937_05790"
     CDS             complement(12178..13512)
                     /gene="dgt"
                     /locus_tag="PK937_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HNH32115.1"
                     /translation="MVQETLHPQKVFFERGRQFVEDCEEQFLAPYACKSKDAHKTRQH
                     GEMEDVHRTAFQRDRDRIIYSNSFRNLSDKRQILVEHDSERNRSRLTHTVEVVQVSKT
                     IAKSMGLNEDLTEAIALGHDLGHPPFGHTGEAILDALLNGEDTAEGTLPGDDFGGFKH
                     NYQSLRVADILEKKYEYEGLNLTSFVREGILKHTSIRSERYRYPDIDTDKLHCDQFVS
                     LTLEGQVAAIADEIAQRTFDLEDALRANYIRLKDVRELKLVQDVDRSCSIAHFYEEDP
                     DEYINFMISGLVRVLVSDVIQNTIRRVEEFCGRKQRTDHFDEWLVIFSLDINRKQNEL
                     ETFIQKEMGNNFLVNRYNKKCEKVIRKLFKAYYSNPLQMNDKFLMRFTQKQVDAKFDL
                     RKLPPKMVQEKVSEFKKNPLYARHIADYIASMSDSFAIQEYKAIYIPEASVL"
     gene            complement(13573..14430)
                     /locus_tag="PK937_05795"
     CDS             complement(13573..14430)
                     /locus_tag="PK937_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNH32116.1"
                     /translation="MARIKMRINSREDQSIERYLEEINKVQLLKPHEEIELAKRIKKG
                     EKEALKKLVEANLRFVVSVAKDYQNQGLPLSDLINEGNLGLMKAAERFDETRGFKFIS
                     YAVWWIRQSILQAIAEHSRIVRLPLNRVGMLNKIGKAYNELEQEFEREPSTEEIAHEL
                     EIKDYDVSDALRISKRHLSLDAPLQEDEKSVLMDTVPDKVNPLPDETLMQESLRRDIE
                     NTLASLSERESKIIKLYFGLGIERSLTLEEIGEEFKLTRERVRQIKEKAINRLRHKSR
                     SKSLRSYLG"
     gene            complement(14786..15472)
                     /locus_tag="PK937_05800"
     CDS             complement(14786..15472)
                     /locus_tag="PK937_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNH32117.1"
                     /translation="MISIIIPTYNEIQNIPIRIEECSALTDAQIILADGGSTDGTAEY
                     VRDAAIRNNHLDWIQSGKGRALQMNAGARLASGAWFVFLHADTSLSQSSWEVLRKTIE
                     EKPQLIGGAFYLQMDHPGWKYNYLAWYSKQRSRLFKTPFGDQAVFVKADIFRALGGYR
                     EDFPIMEDLDFVRRLKAAGDFEMLPAYVVTSARRFAEEGFFRRSCKNLFMQTLYCFGV
                     SPSRLKRWYE"
     gene            complement(15504..16232)
                     /locus_tag="PK937_05805"
     CDS             complement(15504..16232)
                     /locus_tag="PK937_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNH32118.1"
                     /translation="MIKNFCIACWLFSSVVFAQYNDSELYVTVLSQHVHSGKVDYARL
                     RLDRRFSNYLFSLTEVNPDTIQNLNARKAFWINVYNAYTLSVVRDHYPVKSIRAIDTG
                     LFGTSVWDKPYVHVGGKKYSLNDVEHKILRPMNDPRIHFALVCAAKSCPVLRNEPYRA
                     EKLDEQLNEAARQFMNDPERNRVDHQAKALYLSSIFKWYGTDFGKSTGDMVRYLLRYM
                     DINDTDRVVSYRVEYLDYDWSLNE"
     gene            complement(16234..16677)
                     /locus_tag="PK937_05810"
     CDS             complement(16234..16677)
                     /locus_tag="PK937_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNH32119.1"
                     /translation="MKSVFDPAARQEIIDRVNKLSPATQPQWGKMNVAQMLAHCGISF
                     RVPTGDFTIQKTWVRFIGRFFKSLATNDKPFQKNSPTAAEFLITDERIFETEKKNFID
                     NFQKVSRGPEAITYFEHGFFGYLTAEEWGKMIYKHTDHHLRQFGV"
     gene            16932..17297
                     /locus_tag="PK937_05815"
     CDS             16932..17297
                     /locus_tag="PK937_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNH32120.1"
                     /translation="MATKKPAKKKPAKKAAKKTVKKAAKPKKAAKPAKKKTARKPNAA
                     FMAKLTPSATLAAVIGDQARPRTEMIKKIWDYIKKNGLQDKTNKRMINADDKLRALFG
                     GKSQVSMFDLAKLVSKNVA"
     gene            complement(17375..20716)
                     /locus_tag="PK937_05820"
     CDS             complement(17375..20716)
                     /locus_tag="PK937_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH32121.1"
                     /translation="MNALWSLVLRLCFYFTCVASFLSAQTPTSGALNIFVQTGHSSQV
                     LGLDKTSDDRFLVTGSTDATVKLWEIASAKEVRTLRGHTAPVTHVAISPDGKTIVSAS
                     SKDSSVMIWESVSGVRKLKIRLNYEMTAMLVRPTWNSILVSSSEDAVIEYSLQTGKEE
                     RRFQNVGSLLCISDDGKKAVSRLYDRPDGVIVASAERLHIWDLLNGQTIQKLDSSNAM
                     TAAITRDTRYVVTGERNNTIKLWDIMAGRSVRTFGYETEPAMFASDEMDEVISIFFSP
                     DEKKILSAHKLSGYYVWDRKTGEPLRQLHADSYRKVDEMKAENGVVSIRACAFMKDGS
                     TIFGIDGRRIHMWNNDTTEPILRYQGVVQGRINDVRTTPDGGSLILANSENGLRFFDV
                     HSGKPAAAFLSTVSDIQSVAISNNGKFVLLVDQASVASLWDITAKQPLCTFKTEVPFA
                     ALSDDGEIAISANENEFAAYRTRDGVMLSTWRSPHITWSDITFIPNSRRVAIGFWKTP
                     YAPFDGLLTVWDIPSGQLVFELHSEAGTPRCVSVSFDGKMILSGHEDNTLKLWDSHSG
                     KLIRSFNTLIPLAFMKPYEGITAAGLSPDGRWTVSGSADGTIKIWRNDNGTEWRTLKG
                     HTGTIGLVCFSPDSKSAFTAGADGSVRMWDIESGTEYVSFVSLGDADWVTMTREGYFD
                     AGGAGARNINVSAGDQVYTIDNFFETYFRPDIVQAKLAGRAIETMGSSDGLLQGVKIP
                     PTLKLLALDHNGQYAPLVSENEKTANISNGVIRIKIIAEDAGGGVSGIRLFNNGKAVG
                     ENIRGFKATPSIEKIEQEFFVTLSEGENILAAVGFSNDKSESNPVKARLFYRRPVLAK
                     PDMHILAIGINQYRNAKYNLNYCVGDAQGFVNAITPKAKKIFSNIYTTLLLDREANRA
                     GVLAALASIQAAAKPEDVLIIFYAGHGMAMDIEENGPSRNEFFYILSEVTQMSDPMKC
                     AQEGISGTEMRKILSDIKATKQVMFVDACNSGAFAQQFAVRGAAEENALAKLSRASGS
                     VIYASTTKEQFATELAALKHGAFTYVLIDALGGQAGLPNGQLTVSSIKAYVDDKIPEV
                     TKKYKGEEQYPTIFIWGQDFPIGMK"
     gene            complement(20717..23542)
                     /locus_tag="PK937_05825"
     CDS             complement(20717..23542)
                     /locus_tag="PK937_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNH32122.1"
                     /translation="MPSEKSKSQGKKTSSTTPKTMIFGVEDAKKLREAIAANPTVNPS
                     PNEADAKTAESKETAEHKRMKEFTPGVPPWRRWGPFVSERSWGSVREDYSKDGNAWAY
                     FSHDHARSKAFRWGEDGIAGWCDRYQVVTFAPAFWNGKDPILKERLFGLTPLEGNHGE
                     DVKEYYFYLDSVPSHAYNKYLYKYPQAEYPYQWLIDENKRRQGQGEEFELLDTGLFDE
                     DRYFDVFIEYAKAGPEDILIRVEVHNRGDQDAPFHYVPNYWFRNRWGWSKPRTGEPTI
                     VPGPKGKNFVSVISDDTTMETFKNLPFLYQLGKRYLYAEGNPEIYFTDNESHHEKLYG
                     VPSAKPHVKDAFHRYIVNKENCINPDQVGTKSCFHYTDTIPAKSSKVYRLRLTAETLQ
                     SPFEKFDEKVRERQNEADEFYETVYPPKLSADERNVMRQAYASLLWTKQIYLYDVNRW
                     LTGDDPTMPPSESRWTIRNHHWRHLNSMRIISMPDKWEYPWFAAWDLAFHTIAFADLD
                     IEYSKEMLWIMLFEQFQHPNGQIPAYEWEFSDLNPPVHAMAVWKVYTMDRDRTGKPDL
                     VFLEKCFHKLLMNFAWWINKVDASGNNVFEGGFLGLDNITVVDRSEKLPGGAVLEQSD
                     ATGWMGMFCLNLMRICLELARYNKVYEGLATKFVQHYIYVGAAMKNMGEQGIELWDEQ
                     DGFFYDVLVYPNHSGYEKFRVRSLVGLIAMYAIDILNAKDVEANKEFVSNLDWFVRNR
                     PDIVQRCVYIDETDASNRRHILSIVDAHQLQRIMERMCDPEEFLSPAGLRSLSKYHAE
                     HPYYFGEKKVEYEPAEATSKIKGGNSNWRGPIWFPTSYLMINALQKFSEAYGDDFGTT
                     RDGKLIRPKDLAQDIAERMIRIFTRDAKGHRPVYGDIKKFQNDPYWKDYVLFFEYFHG
                     DTGRGLGASHQTGWTGLIATLLQEWRR"
     gene            complement(23872..24393)
                     /locus_tag="PK937_05830"
     CDS             complement(23872..24393)
                     /locus_tag="PK937_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32123.1"
                     /translation="MFHVRFSKVIVIVLCVVLNTGCSSSLSINLENSSNREALNSKIN
                     DRTCEINIKKRGTIKADQVALTDSHIIWSHVKTAQRDSAALKDLIMLSWRNRKEGMYR
                     GSAIGFATGAVGSVAVTKLGYSGVPFSAGDYFLIAISNGLLGGLVGIPAGLLFGKKYE
                     IYPCLPNSKNDCK"
     gene            complement(24637..24711)
                     /locus_tag="PK937_05835"
     tRNA            complement(24637..24711)
                     /locus_tag="PK937_05835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24674..24676),aa:Val,seq:tac)
     gene            complement(24802..25623)
                     /gene="pyrF"
                     /locus_tag="PK937_05840"
     CDS             complement(24802..25623)
                     /gene="pyrF"
                     /locus_tag="PK937_05840"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNH32124.1"
                     /translation="MSFRDRLQQAMQLSPVCVGLDTDMRLIPEFLHSAPNPILEFNCR
                     IVDATIDLVGAYKPNFAFYEGHGVVGMEALRETVAYIRAKNPDIITVADAKRGDIGNT
                     AAMYAQSIFEDLEFDCVTVNPYMGWDSVSPFVKDPEYGAFILCLTSNSGSHDFQYKMI
                     DGKPLYRHVAEKSQLWNTSGNIGLVVGATHPDDMKSVREAAGDMPFLVPGIGAQGGDL
                     ASVVQSNASGETLRALINSSRNIIYASNGKDFEDAARKATEYLRDQIGKLLQSGV"
     gene            complement(25620..26465)
                     /locus_tag="PK937_05845"
     CDS             complement(25620..26465)
                     /locus_tag="PK937_05845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNH32125.1"
                     /translation="MRYHSRFDSYNRWLGTDYPMVKLLISINVVVYFIQYILIFFGLG
                     RWVSMHLALSPYIWDGEIWQLVTYMFLHSANDPFHILFNMLALWMFGKELEMVWGSRE
                     FVKYYFICGVGAGLTFLFFSNGIVVGASGAVFGILLAFGMTFPDQIIMMSFLFPIKAK
                     YMVMIYGVITFMNIANPSGDNIAHLAHLGGMVFGFLYLKKEWFKQKMQETPKPRAAER
                     PPVTPPVMTVQSRPKPPEPKQVIRDRVDEILDKINEVGYDKLTTEEKEILLRASQNYA
                     EQKKP"
     gene            complement(26483..27706)
                     /locus_tag="PK937_05850"
     CDS             complement(26483..27706)
                     /locus_tag="PK937_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH32126.1"
                     /translation="MDEQLRAALGDLIAIEEIVFEWDTAYVALRTPQINTDIMAQIEA
                     RLAPLGLKSSIASINRIPRGMEDTIVLRIEPITAAWNPIRRYTMTQESETPGRWAWLS
                     NPWVNGILFVITFAVVFMTGAATITNRSLSDDWDWVMGFQFSFSLLGILTAHEFGHYF
                     AARYHKLNVTLPYYLPGILIPPIPWLGFPGTTIMPGTFGAFIRIKSPIINKRQLMDVG
                     AAGPIAGFVVCLFVLIYGFATIPDKTWAYQFYNVEEIFSGQPVMYFGDSLLFRWLGHG
                     LAGDRMPAMYDIVHYPFIFAGWFGLLVTALNMLPIGQLDGGHILYALIGSKQRFAAYG
                     AFAVIITIGLFYGAGSWFIWALLILFLIRFKHPRVMDEDEPLDNKRIAIGIVSFLIFF
                     VSFIPLPVYEKIPIP"
     gene            27718..28920
                     /gene="lpxB"
                     /locus_tag="PK937_05855"
     CDS             27718..28920
                     /gene="lpxB"
                     /locus_tag="PK937_05855"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNH32127.1"
                     /translation="MILNLIYIIIAPMHNLTPQHIILLAGEKSGDQYGALLAQRLLSI
                     NPAIRLSGIGGDTMQKAGVQLWENTTHHSVMGFVEVFRSIRYWLKLRKKIIRLILTEK
                     PDAIILIDFPDFNLSVARSIKKTLGERSPFIFYFIPPQVWIWRKKRIHFIAKLCHTVY
                     PLFSFENDLYQSRGIRSRYFGHPIRDMLSAGKYPQNPHQKQDNHITLGLLPGSRNQEV
                     VRILPIMLQSVLLYSQRSRQPLHLYISRVGEVDAFIYETIHRRFPTLNITWCHTGDEA
                     ITHSDAILAKSGTVNLELIYHRKASLIVYRTGTLTYLIARVWLRIRHISLINILAGNT
                     VVREFIQHHARPESIAQEIDRLLHDDVYRHDMLGELDKLHTALFPESESPSAVNQIAA
                     DMLSITSR"
     gene            complement(29035..29985)
                     /locus_tag="PK937_05860"
     CDS             complement(29035..29985)
                     /locus_tag="PK937_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32128.1"
                     /translation="MKSFMLTWFVALALGLSAVNGQDNGVAFLSSIKGTIKVTDASGK
                     EKKASPSLKLLAGDKITASANSSVSVMYYSGKEVVLKAGQNHVITAVKTEDSFLSGLY
                     KSLSGMLWGEASNSSMAGATRAWAGDANRNITAVYPSESKVINDSPVFEWADHRASPG
                     NDYVITISSEISDFKYEMTIKGSKQAAYPKTAPKLKSDEKYQWSIKDATGAEVSPTAK
                     FSLLAPEDKETLEDDIKEVAKVCNNDNTNPQWYLLSAALYRDYGLMKEAETAIKALIA
                     MKPDMVQAHVMLATLYKETGRLEEAKAAEEQARKLSEGKK"
     gene            complement(30111..32228)
                     /locus_tag="PK937_05865"
     CDS             complement(30111..32228)
                     /locus_tag="PK937_05865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH32129.1"
                     /translation="MKLEVKRHNKKLWLSMIVVVAVLVGLNVAHAPAFRSWEESGLDF
                     LFKIRTDVPEQIPVVVVMVDEKAYEKDFGYYDPLPRRYLARLIDTLKAKGAKTIALDI
                     AFYDKLDQLDARGDTLLRDAMHRARNVISVSIWHQREDGSIRIQEPDPFFRPALQAVG
                     YANLQISGGGGLASVREVRPVATMSDNTMLLSFSAAAYCQMMDLPAEEFIRDIRENRW
                     QLLPRIPRDAQGNMLINYISAPSEWVKQEDGTWTQMTPSRITTYRSSQLTGDIEWPED
                     IFKDKLIVVGNGSEFVPDRFITPFFEQTRRNWMYGAEVHANAFLTLLNKSFIDKPGWP
                     VIALCMLLLSITMVMATVRLGFAAEMATLTVLLITVWTGAHILFAGGWWIPAVSLSLV
                     VLLSYFMTSIFQAFTEEHDKKQIKGMFSRYAPPAYVDQLVLDPSKLELGGEEKEISIL
                     FSDIEGFTSISETMAPKQLVELLNDYLNAMTQRIFQQGGTLDKYIGDAIVAVFGAPLP
                     QNEHALHACYAALDMQSSLEEFRAKWMAKGMPAIRNRIGVNTGRVVFGNIGSDIRYDY
                     TGIGDAMNLASRLEAANKQYGTYLMISEFTHAQVRERVIVRELDMIVVKGKTKPVKVY
                     ELMGRANEPLPENTRHLLARYQEGIDLYHKREWSEAIRRFEQALTYAHTDEPSKLYIH
                     RCKQFIAEPPAPDWDGTYHLTSK"
     gene            complement(32256..32813)
                     /locus_tag="PK937_05870"
     CDS             complement(32256..32813)
                     /locus_tag="PK937_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32130.1"
                     /translation="MTLFENHSFMLLIFNALVVFTVLANIYQELYAVIRFSERTMGKA
                     QFYQATAGRLETIVYWCGFFVTLLISALILLSDAENHTNLVACIALAVGFFVNAVVSR
                     ICFFNEIGIGSVNVQSEMEFQWNEIKSFYWQNNVLHLQLRRKLFGHIRMRFSDSTSIL
                     LINERLHRLADSGVSLGTESAPIQG"
     gene            complement(32829..>33221)
                     /locus_tag="PK937_05875"
     CDS             complement(32829..>33221)
                     /locus_tag="PK937_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32131.1"
                     /translation="YNPAGLATLQGRTFAASYKESLPLSLQVGVNYKLQNAPVTVNLT
                     FTDIQNDLVKSPVVGAEWMLHESWSVRLGYSLQRDRELHAGSSDFLDRMAGASAGIGF
                     KYDKYIVDYSFASWGIGTLNRFSIQYQF"
BASE COUNT         8963 a         8231 c         7095 g         8832 t
ORIGIN      
        1 caaaattcca tttattggaa taaagaagtg cgcgattggt tcgcacgttg gttaaaataa
       61 acatttcatt taatcatagc acaaaaggtc aaacgattac gtttgacctt tattttttta
      121 cataatcact atgctgcctt tacttacaaa cgaagaatta tcccaactgc ctccggacgg
      181 cggctctgaa tataaccggc ttatttttga aaaaagcccc tatctgctcc aacacgcacg
      241 taatcctgta aagtggtggt catggtgtga cgaagcgttt gaaacagcac gcgccgaaga
      301 taaacctgtt tttttatcca tcggttatgc gacatgccac tggtgccatg tgatggaaca
      361 tgaatcgttt gaagatgccg aaattgcctc agcgatgaac gcattgttta tctgcgtcaa
      421 agtagatcgc gaagaaaggc ccgacattga ccatgtctat atggccgtca cgcaagccat
      481 gacaggtcat ggcggatggc cgatgacggt attgatgaca tgggataaaa aacctttttt
      541 tacgggaact tactttccta aacgtagccg gcacggccgc atcggcatgc atgatctgat
      601 caaagctatc aatgacgcgt ggctgcaccg taaagacgat gttttgacca acgcccgtca
      661 aatcgttgaa tcagtatcaa gagccgaagc aagcgagcca aaacccttac gtacggcgat
      721 cttgtcggaa gcggtagaga tgtttaaaaa tcaatttgat cagcgctacg caggtttcgg
      781 aaatgcgccc aaatttccca caccgcataa catatcgttt ttactacgat attatcatac
      841 cacgcatgat gcggacgtcc tccaaatggt tgtacgaaca ttggacgcta tgcgatgggg
      901 tggtatttat gatcatatcg gatttggatt tcaccgctac tccaccgatg cacgatggtt
      961 tttaccgcat tttgaaaaaa tgctctacga tcaggcttta acggctatcg cctacaccga
     1021 agcctatcaa ataaccggat acgctcgatt taaagaaaca gcgcagaata ttttggagta
     1081 tgtactacga gacatggaag cgccgagcgg cgggttttat tccgccgaag atgccgacag
     1141 cgatggcgaa gagggaaaat tttatttatg gtcgttcagt gaaatcgcgg ccatacttgg
     1201 aactatagac gctgaattat ttgcaaaggt atacaacctg gatcgtgaag gtaattatta
     1261 tgatgaagcc acacgcgagc ccaatgggtc gaacatcata tttttgacnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tttggacgac tacgcatttt ggatatgggg
     1441 attgattgaa cttgtagaaa ctacaggcga cgtacaattt ctaaaagaag cgcgacgatt
     1501 aacaaccatc gtggttgacg aatttgaaga tccggacaac ggcgggtttt attttacctc
     1561 gcaaaacggt gagcccttga tccgccgtac taaagaacta tacgacggag cgataccttc
     1621 cggtaattcc gtaatgttat cgaacctcta ccgcatgagc cgattgctac acgaaccggc
     1681 cttagccgga aaagcggaac gaatcggttt atccttcgcc gatagcattc atcgcatgcc
     1741 tatggcgttc gcacaatttt tgagtggatg ggatcgactg cagcaagcgc cgatggagat
     1801 cattatcacg ggaacaaaag acaacgcaac atttcaggtc atgtcaactt tggcacatca
     1861 cgcataccat gcgaacaaaa ctttaattat caaatcatcg gatgtctggg atgatttgtc
     1921 accacagtat aaaaattatc cgatcaattc agattatgct aaagcctatg tatgccgcga
     1981 ttttcattgt gaggcaccgg tgtcggataa aaccgatctt caaaataaat tacgcaacgc
     2041 accgtgattt tcatgtcatt aaccaattgc acaatgccag taaatagacg cgaaagcgaa
     2101 ggaacgtagg gttttagaaa tttatttttt caatgaatac gtctattttt tttattctgt
     2161 cagtttctat ccaaccaacg acaaaaaagt cagaaccttt cttgagtata gcatttgcgc
     2221 tactatagcc cgacgtgcca tacattttaa aatctatttg cgctccgcta atcgcgtcaa
     2281 cggtcattac aaacgcatta gtctgacgcg ttgttatttg tcccgctacg gcatagatat
     2341 tatctttgag gatgataccg tgattggttt ctgtacttcc caaaaagcta ttgtgccaca
     2401 tcacattacc atcggcatcc gtacgcaata tcatggtaga aatagcacct gaataaggac
     2461 taagccgaac attgcccgaa acgatataac cgccatccgg caatatactt accgaccttc
     2521 catccgatga tacgcccatg gtatatattt ttttccaaag ctcatcccct tccgaactta
     2581 cccgaattaa taacgtgtga aaaggataaa taccattgga tgaataacct gcaataataa
     2641 aatcaccgtt ttcaagcttc ttaatatcgt tacctacatc tatacttgta ttaccaaatg
     2701 tgcgttgcca aaaaagttga ccttcttttg taatttttac taaaaaaaca tcattacttc
     2761 cggcgccaaa acttgctgtc gttccaacca ctattacaga gccgtcatct gcttgaacta
     2821 cggcgcgacc atcttcatta tagcgaccgc cgaaattgcg cgaccatatt atttcgccgg
     2881 ttttactcaa acgcaaaacc aacatatcta aatttccgct cgaaacctga attgacccga
     2941 ccgctataaa tgaatgatca tcaagttcaa taacattaaa acagcgatca gtcgttttac
     3001 ctccgatcga tttaacccaa actttttgat tattttcgtc gatgcgcata acaagcatat
     3061 cccggttatc gcgaatacta ttggtgcttc ctccgatgat catgccctta tcctgcaaga
     3121 tactcgccga tatggcgtaa tcattctctc caaaatcgaa ggtctgaatt tcgcgggaat
     3181 caggcatatt ttcacaactg caaaacatga aaatacaagt acatacataa agcatgaata
     3241 atctcatgtg atctcctata ctttactcat aaaggcaatg ttatttcaat aacggccaca
     3301 tccgtggtcc ggttatcgct atcaataaca cctcctattg cccaaaattt attttcagtt
     3361 tttatgattg aataacccca acttgtagcg ccatccaagt atcttttaaa accatttgga
     3421 ataccatcgg acaaattcac tgttaaagca tataattgtg tgttcgctcc gtcaataaat
     3481 ccggttacta cggcgttttg ttcatttaac acaaccatgt caaatgattc attattaaca
     3541 aaaatattat aattttggta ccagataata ttaccagtgg catctgcatg aaatacaaat
     3601 gaggaattac taccgggaac atcgctatat tgcgctatac ctgtcacaag aaaactctga
     3661 tcggacaatt ctataacgcg tttagcattc gctttaccgc tcttgggata tgtcctcgtc
     3721 cattgttcct ctccactttc tgaaattttg atcaacatcg cgtcgtatgg cgttatgcca
     3781 tttgccatga aaccggatat aataaatcct ttatctttag ttaattgaat atcgtacgca
     3841 ccgcttacct tttgatcgcc atacgttttt tgccatagta aagctccggc taaattcatt
     3901 ttgaccaaga atacatcatt actaccggcc ccaaaactcg cggttgtacc ggccactatg
     3961 atattaccat catttgcttg tactacggct cgtgcttctt cattataaat cccgccgata
     4021 ttctttgacc atataatttc gccatatttg cttaaccgca caatcagcat atcgttattc
     4081 cctacggatt tctgcactga acctacagcc actaccgagt ggtccgaaag ttcaaagaca
     4141 tcatggcatc gatcaatagc attgccgcca atagctttaa cccacagttt ttggtgagaa
     4201 tcatccaatc gcattaagag catatcctga tttccgcgga tacttgtcgt tgtaccgcca
     4261 atgatcatac cgccatcgct gtacggaatc gcgctgacac catattcttc tttaccaaaa
     4321 tcgaaaaatt cttgggattt attttcaggc caattctcac aagctattac agcaataaga
     4381 aatgataatt ttaggaatct cattttacac ctcaacgtgt tcttggcaaa ttaatttcag
     4441 cacttactcc caacatgaat aatcttggga aataaagctg gtcctcttga gtaaaaggcg
     4501 ctaatgttcc atccgggttg atatgacgtt tccatgaacg ctgccgaaca ttcttatgat
     4561 cggtcacgtt gactacgctg acgtaagtga cccaatgcat ctgctgcccc gtccagcgct
     4621 tttcgagacg taaatccagc gtgtaaaaaa tcggtaaccg tccgctattt ttggtttgcg
     4681 tcgtataata aattttactg ttttctactg tcccatcacc caaaatgcga actgtactcg
     4741 gcgtaaatgg atcgccggat cgaatccgaa atgaactcga taaagccatt cgtgaaggaa
     4801 ggttggaaat attgctgagc aataacgaat gtgttcgatc aagatcgcga taggtctcaa
     4861 gttttctttc attacgaata tggcttgtac tcaatgagta cgccagagaa aacaagttag
     4921 aattaccata cctatgacgc gccaaaagat caaaacccgt tgcgaaaccg gatactgtct
     4981 ggtagggttt atcaacaagc cccacgcctt caatacgatt tgaaaaatca aatgtaaaat
     5041 catcaaaaag ctttggataa tctttataat acgcatccaa cgtaatgttg gtattatcat
     5101 catatttata aataccgcta gcgatgtaat gaatactttt ttccggtgta atatttggga
     5161 cgtgttgatt agcatacgaa cgtaattttt gaaaattatc gggttgataa taccaaccat
     5221 acgcaagtcg taaagtaagg tccgaattaa aatcatagct catggccgct cttggagcca
     5281 attgaatagc attagcataa ctttgacccg tagcgcgtaa accgcctaaa acagtcattt
     5341 tatcattaat tttatatgaa ttttgaaaat aagctgctcc ggtatagccg ctgaatttgc
     5401 tatcaacgaa aataatatcc tgggaaacca aaataggcgt tgtctctgta ggattgagtc
     5461 gaaattcgcg gaaataatat tcactccaaa actgattaaa ttccgcgccg aactctattg
     5521 tgttttgtct gttaaatctc catgagtgat cgtgcttgag tgttaataat tttgtcgtac
     5581 ggttatcttg attatcatcg gtatagctgc tgttaaaaag aaagtctgat ttttttgtca
     5641 aatcctgaaa gcccaatgtg gttgtactat agcgatccgt tgagaccgta ttgagccaat
     5701 tgagccaaag gtaataattc gtttttgtac taaagaaaaa ctcactcgaa tcgctttctt
     5761 tatactgaat attatcattc agcataagat agtttacagt aatccggttt ttaggatcta
     5821 ctttgtaatc agctttaacc catccatccc aaatcgtagt cgggaaatta ttatcaatcg
     5881 cattttcgag taagaaaata taactctttc gcccactggc aaacaccgtt aaccgatcgt
     5941 tgatgcgttg attaagcgat gctgttgcat tactaaaatc cacgcttatt ttcatcgaat
     6001 catcttcaat gcgatcaata gtattaataa acaacagccc cgacatacga tcaccgtact
     6061 tcggtgcaaa acctccggtt aggaccttca tacttttcac aagatcggaa cttatgacac
     6121 tataaggccc gtcaatttct tcaaggtgaa aaggttcacg aatttcgaga ttatcgatga
     6181 taaccgccgt ctcatcagga tttcctcctt tgacgtgcgg tttagagctc cattcgctat
     6241 tggaaatacc ggggaccgtc tgcaatgcac ggtacacatc tttgctgaat acttgaacag
     6301 agttaacaat ctcctgcgtg gaaacggtgg aagaagcgcc ttcgtctata gataattccg
     6361 tcacgccggg cgtgatctcc tgcgcttcga aaacgatcgc ttgctcctcc atcttaatgg
     6421 ggaaattaat atctttgaaa agctgcactt tgcgggtcac acgcttatag cccacgcgcg
     6481 tgagcacaag cgtgtaataa cctttcttaa gatttttgat tgcaaaatat ccggctgtgt
     6541 ctgtgagagt gcctttttga atttcctgaa cgtacacatt gaccgatggc ataggacgcc
     6601 ccgtgtagag atctttgacc gttccgtcca atgtaaacgt cacttgcgct gaaagagaaa
     6661 aaatacccac aagcagaaac aaccccgaaa gacttaaacg atagatcatg cgtacctccg
     6721 ttgttatgac attatatctt aatcttaaac ttgtaaaaca ccaaaattag ggggcggcac
     6781 attgggatta ttgctggccg catctaaaca gcgaatcaac gtgtcgcgag tctccgtagg
     6841 ttcgatgatc gcatcgaccc agagacgtgc tgccgcataa cgcggattac cctgtttttc
     6901 ataattggaa cgaatttcct catagagttt tttcttctct tcttcgctga cttgtttacc
     6961 ggccaatcgg atttctgtaa tcgtcgatgc ggcttgtgct ccgcccatca cggaaatatt
     7021 cgctgtaggc caggcaaaaa taaaccgcgg atcgtatgct tttccgcaca tggcatagtt
     7081 ccctgcacca taacttccgc cgatcacgat cgtaattttg ggcactacgg agttggctac
     7141 ggcatttaca agcttcgcgc cgtctttggc aatgcccgca tattccgcat cgcggcctac
     7201 cataaaacca tttacatcat gcataaaaat cagcggtatg ccgtcctgat tacaattcat
     7261 cacaaatcgc gccgctttgt ccgccgcttc attgtacatc acgccgccca tttgcatttc
     7321 gcctttgacc gtgcgttgga ccagtttttg attggccaca aatccgaccg cataaccgcc
     7381 gacgcgtgcc gtcccgcaaa caacggtttt gccgtaagtc gctttatatt cattgaaatc
     7441 cgatccgtca atcagacgtg caatgatttc acgcatatca tattgaccta gaccttcggg
     7501 taaaatacct gcgatttcat ccatcgccca ttggggtgca acggcagcga ttttgttgaa
     7561 attggctttg gggcgctcgg ctattttgct caagatatcc cggattcggt ccaatgcggc
     7621 gccatcattg ggctcatgat aatccgccgt gccgctgatc gcgttgtgcg tggtcgctcc
     7681 gcctaaagtt tccgcatcga tcacctgtcc gatggccgct ttgactagcg ctggcccggc
     7741 aagaaacaag ctcgcaccct gcaccatgat aaatttgtcg cacattacgg gcaggtaggc
     7801 accgcctgcg acgcacggcc ccatcacagc cgctacttgc gtaatgccca tcgcactcat
     7861 gcgggcgttg ttataaaaaa tgcgaccaaa gtgattttca tcgggaaaaa cctgatcctg
     7921 aagcggaagg aatacaccgg cggaatccac gagatacacg atcggcagaa aatttttcat
     7981 cgcaatttcc tgcgcgcgta atgttttttt gaccgtcatt tcaaaatgcg ctccggcttt
     8041 gactgtcgca tcatgtgcaa cgatgacagt ttctttgccg tgaatcaaac ctatgcctga
     8101 cacacaaccc gccgacgggc aaccgccaaa atcagtatac atttcgtaag ccgcaaaaag
     8161 ccccagttcc atgaaatggg catttggatc caaaagttta acaatacgct cacgggccgt
     8221 gagccgtcct ttttcatgct gtttatcaat atttttttgg ccgccaccgc gtttgatcgc
     8281 ctcgccatcc tgacgaaggg cattcagcaa acgttccata aagctacgat tcttctgata
     8341 cttttccggg ttccactgac cggccggtga tcctatcacg ggcatactgc actccttata
     8401 cacacaggct gatgatttat ttcgttgctt ccaaaagttt agccacactt tgcatccaga
     8461 tgtcggtgtt cccgcctgac tcatagatca tatattttcc gtcatgcgtc catcgaccgc
     8521 gacgctcttg atggttattg tctttggaca aagtacgcga cgttttttct ccttcgtcgg
     8581 ttttataaag ccaaatatcc gcatcgtcat accacgtacg cgtgatggca agccattggc
     8641 cgtcgggtga ccaactcggt tcccgatcgg agccgtaggg cgttttatcg cgcgtcacag
     8701 gtgaaggcat tcctccggaa acaggcacgc gccaaatgct ccagcgaccg gcccaatcgg
     8761 cgtcaaaata aatatacgcg ccatcgtttg aaaaaacggg gttcgcttcg gttcgcatat
     8821 ctatggtcaa ctgtttctct tttttggtcg cggtatcgta cacccaaata tcgggactgc
     8881 cgccttttag ggaaacatat gtgatcgctt tgccgtctat agcataatgc gctgcatagc
     8941 ggtgtacgct gtcttgaaat aaagcgtagg ctgattttcc ggccgcatac tcctcaatca
     9001 gattggcttg cgtgatgggc atttcataca gcgtggataa cccgccgcga accacggaaa
     9061 ccaccgcggc cgtgccgtca tttcgcaagt cgcattcggt cacatcggca tccggccaat
     9121 aggcgatgcg aattgcttta ttggcgctca gattacgcgc atacagcgcg tagcggcctt
     9181 ccgcattgct cacatacaac agagttttgc catccgccaa catgaccgga gaacgatcat
     9241 cgccattggt ctcgatcagc atttgttcca tttgtgtttt gacttcgata cggcggatat
     9301 catacgaggt ctctgcctga taggccatcg catgcccgtc tggcgtccac accggaaaat
     9361 ccaaatgttt ggattcgccg ctgatcagca ccggattgaa cgtatcggca cgtgtaaaaa
     9421 aaatctgcgc atagccgttg gtatcggaag aaaagaacaa atgcttgcca tccggagaaa
     9481 gcgccgcatg acgcgcatca cgatatccgt cactccatcg tgcgacggac cctgtcgccg
     9541 gatccaaacg catcaaaacc cacgatgctt cgtttttttc accaaacaca attttgtctt
     9601 tagcgatagt aattttttcc aatggcccgc ggctgcgata aataacttct gatttggaaa
     9661 aattacgggc ttcggttcga tggatggcat atccgccgtc ggccgccgat ttgcttatgg
     9721 ctatatacca aatatacatg ccatcgggcg aatacaccgg aaaaatttcg tcaaccgaat
     9781 ccgttttggt aacctgaatc aattcatcat tgggagagac aatccacaag tcgttttgtc
     9841 cgttgagacg tgacgtgaaa atgatgcgct ctccgtcagg tgcaaacgac ggatgctcat
     9901 caacaccttc attgacggtt acggggatcg taatacgttc gcccaaatcg taaagccaaa
     9961 tgtgccgatc gccggtttgc gtagtgacaa atgccatcac atcgccattc ggtgaaaatg
    10021 ccggctgacc ttcataacac gataaggtcg taatttgttc ctgattaaag ggattgcaac
    10081 cggctgatac gacgaccagt gcgacaagaa aaaaactttt gggcatgtgg gacgttacga
    10141 tttcaaatga aaatattgct gacgcaacat acacaaaacg atagaaaagc tctacagtta
    10201 ttttttgaaa aagcccctca ggcgatacgc aacgaagctt tgggcgatag acccaaatta
    10261 tcggtacgcc ccatacgata atgcatcaga cccgtgatac cgatcatcgc ggcgttatcc
    10321 gtacaatact ggcgggcggg catgaatagt tgtactccgt cctgtgcaca tacctccgta
    10381 agcctggcgc gtaagcgact attggccgca acaccgccgg ataatacgca ttgacaaata
    10441 cccgtcgttt ttaaggccgc acgtgttttt ccgactaaaa catctactgc ggcttcctga
    10501 aaactcgcac acgcatccgc cagcaaattt tgtgcaagcg gttgattttg cttttgcaca
    10561 aaatggagaa ccgaggtttt taagccgctg aaactaaaat tcaaatcgcc ggactggagc
    10621 atcggtctgg gaaaggcgat cgctttagga ttgccttgcg atgcaaggcg atctataacc
    10681 ggcccggcca tcaaagcgcc ttctaccggc gttaaaccta gcaatcgtgc ggttttatca
    10741 aaacactcac cggccgcgtc atctacggtt tcacccaaaa tacggtattg acccggattt
    10801 tgcgcttcca ccaataacgt atgcccgccg gacacgatca gtgcgatgaa gggaaaaacc
    10861 ggtttgtctt tttctaaaaa actggccaat acatgcgctt cgatatgatt gactccgacg
    10921 agcggttttt gtaaaccaaa ggccatcgct ttggcaaaat tgacaccgac taaaagactg
    10981 ccgatcaggc cggggccttt ggtcaccgcg atcaatccga tgtcatcagg cgtgatatag
    11041 gccgccgaaa gcgcggcccg gataacgggt acgatattgg cctgatgtgc gcgggaagcc
    11101 agttccggga caatgcctcc gaaacgaagt ccgtcaattt gcgacgatac gatattggat
    11161 aataccgacg atccccgcaa aaccgccgcc gacgtatcat cgcaggaagt ttcgatcgcc
    11221 agtgtgatca gatcatcgtt cgaggctatc atcttcgata cgcacgaggt taaatcgcga
    11281 cggtgtgact tgcgtccagg taagatccgc gggaacgcgg atgtccagac gcggttggac
    11341 ggaattgtat tctttttgat aatcaatttc tccgacgacg ctctctttag gcaatgttga
    11401 aagatagtcc acaccgccgc gtacacgtac ggaaaaagtg gacggttgta ccgccaccgt
    11461 cgtaaaaggt ggcacattga tcaatcgcac gggcaaatta tccagtacga tttcgcctaa
    11521 aggctgtaca tccagaaaaa gtgaagttgt ggattggagt acgttgatgc gtctatccgg
    11581 atgcgctgtg agtcgcaact ccgtgcgcac atcgctattg agattttccc agcgatgcga
    11641 agcggtgccg ataacaaccg cactgtcacg gtaactccag ggcattgtca ccgtcactga
    11701 atccggcatg agctgcatgc cgccgactaa tataaaaccg gcttcgcatg tgatctgcac
    11761 atccgctacc accggaacgc ggcgcgcgat catacggtca attcgtacga tgaccgaatc
    11821 cggccattgt acggcgatgg cgtgcaccgg cgctttggag ggatacacga tttcgtgaag
    11881 atgttccgaa agtataaaac ggcttttatc tttgtgctgt gccatatcca aaacaaaacg
    11941 cggcggattg agaaagcgca taccaagcag ggatttgccg tcaccttcaa aaagaatatc
    12001 gacggcctcc ggaatatcgg agaccacgcc gtaaccgtct ttgatattgg tcacttgaaa
    12061 cgggacgtgg atctccatct ggtatgtcgc gccgagacgc acgtataacc aaagaaatat
    12121 ggcgatgcac agcgacagaa aaaaaatgcg atggcttttg agaaggcgca tggcagttta
    12181 taataccgaa gcttcgggaa tgtagatagc tttgtactcc tgaatcgcaa aactatcgct
    12241 catactggcg atatagtccg cgatgtggcg cgcatagagc ggattctttt tgaactcgct
    12301 gactttttcc tgcaccattt ttggggggag cttgcgcaga tcaaacttag cgtctacctg
    12361 tttttgcgta aaccgcatca aaaatttatc attcatctgc aaaggattgc tgtaatacgc
    12421 tttgaacaac ttacgaatga ctttttcgca ttttttattg tagcgattga ccagaaaatt
    12481 attgcccatt tctttctgaa taaacgtttc cagttcgttc tgcttacggt tgatatcaag
    12541 cgaaaaaatc acgagccatt cgtcaaagtg atcggtgcgc tgcttacgtc cgcagaattc
    12601 ctccacccgg cgaatggtgt tttgaatcac gtcgctcacc aaaacacgca ccaatccgct
    12661 gatcataaaa ttgatgtatt catccggatc ttcttcataa aaatgcgcga tactgcacga
    12721 acggtccaca tcctgcacga gctttaattc gcgcacatct ttgagacgaa tgtagttggc
    12781 acgcaatgca tcttcgagat caaatgtgcg ctgggcgatt tcgtcagcta tcgcggcgac
    12841 ctgtccttcc agcgtcagcg aaacaaattg atcacagtgc agtttgtccg tatcaatatc
    12901 aggatagcgg tatcgttcgg agcgaatact ggtatgcttg agaatgcctt cgcgtacaaa
    12961 agaagtcaga ttaagtcctt cgtattcgta ttttttttcc agaatatccg ccacgcgcaa
    13021 actttgatag ttgtgcttaa atccgccgaa atcgtcgccc ggaagtgtac cttcggcggt
    13081 atcttcgccg ttgagcagcg catctaaaat cgcttcgccg gtatgcccga acggcggatg
    13141 gccgagatca tggccgagcg cgatggcttc ggtaagatct tcgttgagac ccattgattt
    13201 agcgatagtt ttggaaactt gtacgacttc tacggtatgc gtcagacggc tgcggtttcg
    13261 ttcgctgtcg tgctcgacca aaatttggcg tttgtcggac agattgcgaa acgaattgga
    13321 ataaatgata cgatcgcggt cgcgctgaaa cgccgtgcgg tgcacgtctt ccatttcacc
    13381 atgctggcgc gttttatgcg catctttgct tttacaggcg tacggcgcga gaaactgttc
    13441 ttcacaatct tcgacgaact ggcgtccgcg ctcaaaaaaa actttttgcg ggtggagtgt
    13501 ttcctgaacc atagttaata ctttcgatca aaaaagaagt cccgacgctt agcatcggga
    13561 cccgagacaa cattaaccca gatatgaacg cagcgactta ctgcgggatt tgtggcgcag
    13621 gcgattgatg gctttttcct tgatctgccg tacgcgttcg cgggtcagtt taaattcttc
    13681 cccgatctct tcgagtgtca gcgaacgctc gatgccgaga ccgaaatata atttgataat
    13741 tttggattcg cgctcactca gcgaagccag cgtgttttcg atatcgcggc gcaacgattc
    13801 ctgcataagt gtttcgtccg gaagcggatt gactttatcc ggcacggtat ccatcagtac
    13861 gcttttttcg tcttcctgca gcggcgcatc caaggacaag tggcgcttgg aaatacgcaa
    13921 cgcatcggat acgtcataat ctttgatttc gagttcgtgc gcgatttctt ccgtactcgg
    13981 ttcacgctca aattcctgtt cgagttcgtt atacgcttta ccgattttat tgagcatgcc
    14041 gacacggttg agcggcagac gcacgatacg ggagtgctcg gcaatagcct gcaaaataga
    14101 ctgacgaatc caccacacgg cgtacgaaat aaatttgaac ccgcgcgttt cgtcgaaacg
    14161 ttccgctgct ttcataaggc cgagattacc ttcattgatc agatcgctca aaggcaaacc
    14221 ttgattctga taatccttgg ccacactcac cacgaaacgt aaattggctt ccaccaattt
    14281 tttgagcgct tccttttctc cctttttgat ccgcttggca agttcgattt cttcatgcgg
    14341 ctttaaaagt tgtactttgt ttatctcttc cagataacgc tcgatagact gatcttcccg
    14401 gctgttaatg cgcattttaa tgcgggccat tatacattct ctttcgtgtt aagtttatat
    14461 tttacagata ctttaattct tggttgcttt cattaaacgc ccgaaagttc cacttagttc
    14521 catttttttt ggaaatttga aaataaataa tgttacgctt tccggataac gaaacacagt
    14581 gaactttatt gactgatatg agttaaaaag cggacgaaag gtttgatctg aatgaaggat
    14641 cgggagaata tgaaatatta tattattaag tcgtaaaatg atgtgttttc tgataacctg
    14701 gccatgctaa ggtatttgac cgaaaaatga actttagttc ttaaattagt tatactcgtt
    14761 ttttcaattt cgttcaaatt atgttctatt cataccatcg ttttaacctc gacggcgaaa
    14821 cgccaaaaca atacaacgtc tgcatgaaaa gatttttaca actgcgcctg aaaaaacctt
    14881 cctccgcaaa gcgacgcgcc gacgtcacca cataagccgg cagcatttca aaatctccgg
    14941 ctgcttttaa tcgccggaca aaatccaaat cctccatgat cggaaaatct tcacggtatc
    15001 cccccaatgc acgaaatata tcggctttca caaatacggc ctgatcgcca aatggtgttt
    15061 tgaataaacg gctccgctgt tttgaatacc acgccaaata attgtatttc caccccggat
    15121 gatccatttg caaataaaaa gcaccgccaa ttaattgtgg cttttcttcg atggttttac
    15181 gtaatacctc ccaactgctt tgagataaag aagtatccgc atgtaaaaaa acgaaccatg
    15241 cccctgaagc caaacgggct ccggcattca tctgtagggc acgcccttta ccggattgaa
    15301 tccaatctaa gtggttattc cgtatggcgg catcacgaac gtattccgcc gtgccatcgg
    15361 tactaccgcc gtcagcaagg atgatttgtg catccgtcaa tgcactgcat tcttcgatcc
    15421 gaatagggat gttttggatt tcgttatacg taggaatgat aatagaaatc aaatagccgt
    15481 accttgccgc cggatgcggc cacttattcg ttgagtgacc agtcgtagtc gagatattct
    15541 acacgataag acaccacgcg atccgtatcg tttatatcca tataccgcaa aagatacctg
    15601 accatatccc cagtactttt gccgaaatcg gttccgtacc atttgaaaat ggacgacaag
    15661 tataaggcct tggcttgatg atctaccctg ttgcgttccg ggtcgttcat aaattgacga
    15721 gcggcttcgt tcaactgttc atccaatttt tcggcacgat acggttcatt gcgcagaacc
    15781 ggacaacttt tagccgcaca taccagggca aagtgaatgc gcggatcatt catcggtcgc
    15841 aagattttat gttcgacatc attcagagaa tatttctttc cgcctacatg gacatacggt
    15901 ttatcccaaa cactcgtacc gaataatccg gtgtcaattg cgcgaatact tttgaccgga
    15961 taatggtcgc gcaccacgct cagcgtatag gcgttatata cgttgatcca aaatgctttc
    16021 ctggcgttca aattttgaat ggtatcggga ttcacttccg tcaggctgaa aagataattg
    16081 gaaaatcgac gatccaagcg taatcgtgca taatccactt ttccggagtg aacatgttgc
    16141 gataaaacgg tgacgtataa ttcactgtca ttgtactgcg cgaaaacgac ggatgaaaaa
    16201 agccagcaag ctatacaaaa atttttaatc atattatacg ccaaactgcc gaagatgatg
    16261 atcggtatgt ttgtagatca ttttgcccca ttcctccgca gtcagataac cgaaaaagcc
    16321 gtgttcgaaa taagtgatcg cttccggacc gcgactaact ttttgaaagt tatcaataaa
    16381 attctttttt tcagtttcaa aaattcgttc atcggtgatc aaaaattccg ctgcggtagg
    16441 tgaatttttt tgaaacggtt tatcgtttgt cgccagcgat ttaaaaaaac ggccgataaa
    16501 tctgacccat gtcttttgga tcgtaaagtc gccggtgggg acacgaaaac taatgccgca
    16561 atgcgccagc atttgcgcca cattcatttt accccattgg ggctgtgtcg cgggcgagag
    16621 cttatttaca cgatcaatga tttcctgacg cgctgcagga tcaaatactg atttcatgtt
    16681 ttttccttaa gtttggtata aaaaaattac gtgcattttt ttataaatta tttagacgat
    16741 gcccttcaca tcccacgaag aaatgaagcg atgttatcca ttaattatca ttttggcaat
    16801 tatttttcat aatgggcaac gggcaagttt ttttaatttt tttaattttt actttgaaaa
    16861 attccttttt ttacgagata atatagggtc atttaactta ctctcactaa ctaaacaacg
    16921 gaagtaccac catggcaaca aagaagcctg ccaagaaaaa accagctaaa aaagctgcga
    16981 agaagaccgt aaaaaaagct gctaaaccga agaaagcagc caaaccggcg aagaaaaaaa
    17041 cagcccgtaa acccaatgcg gcgtttatgg ctaaactcac gcccagcgcg actttagccg
    17101 ctgttatcgg cgatcaagcc cgtccccgta cggaaatgat caaaaagatc tgggactaca
    17161 tcaagaagaa tggtctccag gacaaaacga acaaacgcat gatcaatgca gacgacaaac
    17221 tccgcgccct tttcggcggc aagagccaag tctccatgtt tgatctcgcc aagctcgttt
    17281 ccaaaaacgt tgcttaatcg cgatttgcat ccgataaaaa aacccgctca caaagcgggt
    17341 tttttgtttt gtatgcgaca cgtatcgatc ctgattactt cattcctatc ggaaaatcct
    17401 gaccccaaat aaaaatcgtc ggatactgct cttcaccctt atactttttt gtgacctccg
    17461 gaattttatc atccacatac gctttgatac ttgagacggt caattgacca ttgggtaaac
    17521 cggcttgacc gcccagcgca tcaatcaaaa cataggtaaa agcgccgtgt ttgagtgcgg
    17581 ctaactccgt ggcaaattgt tccttcgtgg tggatgcgta gatcaccgac cccgaagccc
    17641 ggctcagttt ggctaaagca ttttcctccg ctgcaccgcg cacggcaaac tgctgggcaa
    17701 acgcacctga attgcaggca tccacgaaca tgacttgttt ggtcgctttg atatcagaca
    17761 aaattttacg catttcggtc ccggaaatac cttcctgcgc acacttcatc ggatccgaca
    17821 tttgcgtaac ttcgctgagg atataaaaaa actcattccg cgatgggccg ttttcttcga
    17881 tatccatggc catgccatga cctgcataaa aaataatcaa cacgtcttcc ggtttcgcag
    17941 cggcttgtat ggatgccaat gccgccaaaa caccggcccg gttggcttct cgatcaagta
    18001 aaagcgtggt atatatatta gaaaagattt ttttcgcttt gggcgtaatc gcattgacaa
    18061 aaccttgcgc atcaccgaca cagtaattca gattatattt ggcattacga tactgattga
    18121 taccgatagc caaaatatgc atatccggtt ttgcaagaac aggtctgcgg tagaagaggc
    18181 gcgctttgac gggattggat tcggatttat cgttcgaaaa accgacagcc gccaatatat
    18241 tttccccttc ggacaaggta acgaaaaatt cttgctcaat tttttcgatg ctgggcgtcg
    18301 ctttaaaacc gcgaatattt tctccgaccg ccttgccgtt gttgaacagt cggatacccg
    18361 atacaccgcc gccggcatct tcggcgataa tttttattcg gatgaccccg ttgctgatat
    18421 tcgccgtttt ttcgttttcg gaaaccagcg gagcatactg accgttatgg tctaatgcca
    18481 gtagtttaag cgtcggcggt attttaacgc cttgaagcag gccgtccgag ctacccattg
    18541 tttcaatagc acgacccgct aacttcgctt gtacaatatc cggccgaaaa tacgtttcaa
    18601 aaaaattatc aatcgtgtac acctgatcgc cggccgaaac attgatatta cgagcgcctg
    18661 caccgccggc atcaaagtaa ccttcgcgtg tcatggtgac ccaatccgca tcaccgagcg
    18721 aaacgaatga aacatactcc gtcccggatt cgatgtccca catgcgaaca ctaccatccg
    18781 cgcctgccgt aaatgcgctt ttgctgtccg gagaaaaaca taccaatcct atcgtgccgg
    18841 tatgtccttt taatgtgcgc cattccgttc cgttatcatt acgccaaatc ttgattgttc
    18901 cgtccgcact tccggacaca gtccaccttc cgtcgggtga aagacctgcc gccgtaattc
    18961 cctcatacgg tttcatgaaa gccagcggga tcaacgtatt aaaactgcga atcagttttc
    19021 ccgaatgact gtcccatagt ttcagggtat tatcttcatg acccgaaaga atcatttttc
    19081 catcgaagct gacggagacg caccgcggtg ttcccgcttc actgtgcaac tcaaatacca
    19141 attgcccgct tggtatatcc cacacggtta ataacccgtc gaacggcgca taaggcgttt
    19201 tccaaaaccc gatggccacc cgccgcgaat tcggaatgaa cgttatgtca ctccaagtga
    19261 tgtgaggcga tcgccatgta ctcaacatca caccgtcgcg ggtccggtaa gctgcaaatt
    19321 cattttcatt ggccgaaata gctatttcgc catcatcgga caatgcagca aatgggactt
    19381 ccgttttgaa ggtacacaaa ggttgtttgg ccgtgatgtc ccacagtgat gccacagatg
    19441 cctgatccac caacaaaaca aacttcccat tgtttgaaat cgcaacactt tgtatgtcac
    19501 tgacggtcga aagaaaggcc gccgcaggtt ttccggagtg aacatcaaaa aaacggagcc
    19561 cgttttcgga atttgctagg atgagagatc cgccgtccgg ggttgtgcgt acgtcattta
    19621 ttcgaccttg cacgacacct tgataacgca ggataggttc cgtcgtatca ttgttccaca
    19681 tatggatacg acggccgtct ataccaaaaa ttgtagagcc gtccttcata aatgcgcagg
    19741 ccctgatact gacaacccca ttctcagcct tcatttcatc gaccttacga tacgaatccg
    19801 cgtgcagttg gcgcaagggt tcgccggtct ttctatccca tacataatac cctgataatt
    19861 tatgagcgga tagaattttc ttttcatccg gagagaaaaa aatcgaaata acctcatcca
    19921 tttcatcact ggcaaacata gccggttcgg tttcataacc gaatgtgcgc acacttcgcc
    19981 ccgccatgat gtcccaaagt ttgatcgtat tgttcctttc gcccgttacg acataacgcg
    20041 tatcgcgcgt aatggcggcc gtcatggcgt ttgacgaatc caacttctgg atggtttgcc
    20101 cgttcagtaa atcccatatg tgcaatcgct ctgccgatgc gacgatcaca ccatcgggtc
    20161 ggtcatagag gcgggagacg gcttttttac cgtcatccga tatacatagg agagaaccga
    20221 cattttgaaa acgtcgttct tcttttcctg tttgtaaact gtattcgatg accgcatcct
    20281 ccgatgacga taccaaaatc gaattccatg tgggccgcac cagcatggcg gtcatttcgt
    20341 aattcaaccg tattttcaat tttcgaacac ccgaaacact ctcccagatc atgacactcg
    20401 aatctttcga cgaagccgaa acaatggttt tcccgtccgg agaaatggcc acatgcgtca
    20461 cgggcgcggt gtgtcctcgc aacgttcgca cctccttggc ggacgcgatt tcccaaagtt
    20521 tgacggtggc atcggtgctt cccgtcacca aaaaacgatc atcggatgtt ttgtcaagcc
    20581 cgagtacttg gctcgaatga ccggtttgaa cgaatatgtt gagagcgccg gaagttggag
    20641 tctgggccga taaaaatgaa gcgacgcatg tgaaataaaa gcacaagcgt aacaccagag
    20701 accacaatgc attcatttat cgtctccatt cctgcagcaa cgtcgcgatc aggcctgtcc
    20761 atcccgtctg atgggaagcg cccagcccgc gcccggtatc gccgtgaaaa tattcgaaga
    20821 ataatacata atctttccaa tacggatcgt tttgaaactt cttaatgtcg ccatagacag
    20881 gacgatgccc cttggcgtcg cgtgtaaaaa tgcgaatcat ccgctccgcg atgtcttgtg
    20941 ccagatcttt ggggcgaatc aatttgccgt cgcgtgtggt tccgaaatca tcgccgtacg
    21001 cttcggaaaa tttctgcaaa gcgttgatca tcaggtagct cgtcggaaac cagatcgggc
    21061 cgcgccaatt ggaattcccg cctttgattt tgctggtcgc ctcggccggt tcgtattcca
    21121 ctttcttctc accgaaataa tacgggtgct ccgcatggta tttggacaaa ctgcgcagtc
    21181 ctgccggtga taaaaattcc tccggatcgc acatacgctc catgatgcgc tggagctgat
    21241 gcgcatccac gatggacaaa atgtgacgcc gattggaagc atccgtttcg tcaatataca
    21301 cgcagcgctg gacgatgtcc gggcgattgc gtacaaacca atccagattg gaaacaaatt
    21361 ctttattcgc ttcgacatct ttggcattga gaatgtctat cgcgtacatg gcaatgagac
    21421 cgaccaaaga acgcacgcga aatttttcgt aaccgctgtg gttgggatat accaatacgt
    21481 cataaaaaaa tccgtcctgt tcgtcccaca gttcgatgcc ttgctcgccc atgtttttca
    21541 tcgcggcgcc gacgtagatg tagtgttgga caaattttgt ggcaaggcct tcatagactt
    21601 tattgtagcg cgccagttcg agacagatgc gcataagatt gaggcaaaac atacccatcc
    21661 atccggtcgc atcggactgc tccagtactg caccgccggg cagtttttcg ctgcgatcca
    21721 cgacggtgat attatccagt ccgagaaatc cgccttcaaa cacgttattg cccgacgcgt
    21781 ccactttatt gatccaccag gcaaagttca tcagcagttt gtgaaaacat ttttctaaaa
    21841 ataccagatc gggtttgccg gtacgatcgc gatccatcgt atagactttc cacacggcca
    21901 tggcgtgcac cggcggattg agatcggaaa attcccattc gtaagccggg atttgcccgt
    21961 tgggatgctg aaattgctcg aaaagcatga tccacagcat ttctttggag tactcgatat
    22021 ccagatcggc aaaagcgatc gtgtgaaacg ccagatccca cgcggcaaac cacggatatt
    22081 cccatttgtc gggcatggag ataatgcgca tcgagttcag atggcgccag tggtgattgc
    22141 gtattgtcca gcgcgattcg gagggcggca tcgtaggatc atcgcccgtc agccagcgat
    22201 tgacatcgta caggtagatt tgtttggtcc acaaaagact tgcgtaggcc tgacgcatga
    22261 cgttgcgttc atcggcgcta agtttgggcg gatacacggt ttcataaaat tcgtccgctt
    22321 cgttttgacg ttcgcggact ttttcatcaa atttttcaaa cggcgactga agcgtctccg
    22381 ccgtcagccg caaacgatac actttactgc ttttggccgg tatcgtatcc gtgtagtgaa
    22441 aacacgattt cgttccgacc tgatccgggt tgatgcaatt ttctttgtta acgatatagc
    22501 ggtgaaaagc atctttgacg tgcggcttgg cgcttggtac gccgtagagt ttttcgtgat
    22561 gggattcgtt atccgtaaaa taaatttccg gattgccctc ggcgtagaga tagcgtttgc
    22621 cgagttgata cagaaaagga aggtttttaa acgtctccat cgtcgtatcg tcgctgatga
    22681 cgctgacgaa gttttttcct ttcggacccg gtacgatggt cggttcgccc gtacgcggtt
    22741 tactccagcc ccagcggttg cgaaaccaat agttgggcac gtagtgaaac ggtgcatcct
    22801 gatcgccgcg gttatgcact tctacgcgaa taagtatatc ctccggcccg gctttggcgt
    22861 attcgataaa aacatcaaag taccgatctt catcaaaaag acccgtatcc aaaagttcaa
    22921 attcttcccc ttgcccctga cggcgtttgt tttcgtcgat cagccactga tagggatatt
    22981 ccgcctgcgg gtatttataa agatatttat tatacgcgtg cgacggtacg ctgtcgagat
    23041 aaaaataata ttctttcacg tcttcgccgt gattgccttc cagcggcgtg aggccaaaca
    23101 agcgttcttt caaaatcgga tctttgccgt tccaaaatgc cggagcaaac gtcacgacct
    23161 gatagcggtc acaccatccg gcgatgccgt cttcgcccca ccgaaaggct ttgctgcgtg
    23221 catggtcatg cgaaaaatac gcccatgcat tgccgtcttt gctgtagtct tcgcgcaccg
    23281 aaccccagga gcgctccgac acaaaagggc cccatcggcg ccacggcgga acgccgggcg
    23341 taaattcttt catccgcttg tgttcggcgg tttctttgga ctccgccgtt ttggcatccg
    23401 cttcattggg tgaaggattg acggtcggat tggcggcaat ggcttcgcgc agttttttgg
    23461 catcttctac gccgaaaatc atcgttttgg gcgtggtcga agacgttttt tttccttgcg
    23521 acttggactt ttcagaaggc atggaacctc gcagtgtaaa actttatgag aacgtataaa
    23581 tacttaggta gacggagatt ggtacaggta gtttatctga ccgggcgaag aaattcaaga
    23641 ggaaaacttc gaaggggaat tggtttcaaa aaaaaagtga atgaattatt aggactgaga
    23701 cgcagacatc atctaacgct atttacatca aaactcccat tctctattat aaatttgcac
    23761 ctgattgcta catgagcacg gcgtagccta catgaaaaga aacaacatgc gtctgcgcac
    23821 ataagacgaa tgaaataggt aaacaaactt tttaaattca aaattctacg attatttgca
    23881 atcatttttt gaattcggca aacagggata tatttcgtat ttttttccga aaagtaatcc
    23941 cgccggaatt ccaaccaatc ctcccaataa accattagaa atagcaatca ggaaataatc
    24001 accggcagaa aaaggaacac cggaatatcc aagttttgtc acggctacgc ttccaaccgc
    24061 tcctgttgca aatccgatcg ccgagccgcg atacatacct tccttacgat tgcgccatga
    24121 aagcatgatc aaatctttta atgccgcgct atctctttgt gcggttttca catgggacca
    24181 aataatgtgt gaatccgtaa gcgcaacttg gtcggccttt atcgttcccc tttttttgat
    24241 gtttatttca catgtacgat catttatttt agaattaagc gcctctcgat ttgatgagtt
    24301 ctccaaattg atggaaagcg aactcgaaca acctgtatta agaaccacac acaaaacaat
    24361 aactatcact ttgctgaagc gaacatgaaa catgagatca tttctttctt aaaattgaat
    24421 ttcagctaca aaatagctga agtataaaaa ttgatgttaa ttattttaat tctttgttaa
    24481 gttgcgtgat cttcaacagt gaatgcaaca agggttattc acaaatctct atactcaaaa
    24541 actcttttca aatcgggact ctgacgcaat ctatcaatag tcagcatcta tatcgttaaa
    24601 aacaaaaacc tccgattata atcgaaggtt ttatcgtggg cgctcatgga ttcgaaccaa
    24661 ggaccttctc ggtgtaagcg agacgctcta aaccagctga gctaagcgcc cgaaaaacgt
    24721 ggggcaacaa gacgtcacgc caacagcgtg actaagcgcc cgaagtatgt acttcaaaaa
    24781 acgcggggcg aatataatac ctcaaactcc cgattgcaag agttttccta tctgatcacg
    24841 taaatattca gtcgccttac gtgccgcgtc ttcaaaatct tttccgttgg aagcataaat
    24901 aatattacgg gacgaattga tcaatgcacg cagcgtctcc cccgatgcgt tggattggac
    24961 gacgctcgcc aggtcgccgc cctgcgctcc gatgcccggc accaaaaaag gcatatcccc
    25021 cgccgcttca cgaacggatt tcatatcgtc cggatgcgtc gcaccgacga ccaacccgat
    25081 attgccggat gtattccata attgcgattt ttcagcaaca tgccgataca aaggttttcc
    25141 gtcgatcatc ttgtattgaa agtcgtgcga accgctattg gacgtcaggc ataaaataaa
    25201 cgccccgtat tcgggatctt tgacaaaagg cgacaccgaa tcccatccca tatacggatt
    25261 gaccgtcaca cagtcaaact caaggtcttc aaaaatagac tgcgcataca tggccgccgt
    25321 attgccaatg tcaccgcgtt tggcatcggc cacagtaatg atatccgggt ttttggcgcg
    25381 aatataggcc accgtctcgc gcaacgcttc catgcccacc acaccatgcc cttcataaaa
    25441 agcaaagtta ggcttatatg cgcctaccaa atctatcgtc gcatccacga tgcggcagtt
    25501 aaattccaaa atcggattgg gagccgaatg aagaaattcc ggtatcaacc gcatgtccgt
    25561 atcaagcccg acacacaccg ggctcagctg catcgcctgc tgcaaacgat cgcgaaagct
    25621 catggttttt tttgctccgc atagttttga ctcgcacgga gtaatatttc cttttcctcc
    25681 gtcgtcagtt tatcgtagcc tacttcattg attttatcca atatctcatc cacccgatca
    25741 cgaataacct gcttgggttc aggcggtttg ggtcggctct gaacggtcat tacgggcggt
    25801 gttaccggag gacgctctgc ggcgcgcggt ttcggggttt cctgcatttt ctgcttgaac
    25861 cactcttttt tcaaatacaa aaatccgaaa accatgccgc caagatgcgc aaggtgggcg
    25921 atattatcac cgctgggatt ggcgatattc ataaacgtaa tcacaccgta gatcatcacc
    25981 atgtatttgg ctttgatggg aaataaaaag ctcatcatga tgatttgatc gggaaaggtc
    26041 ataccaaaag ccagcaatat gccaaacacc gcacccgacg cgccaacgac aatgccgttt
    26101 gaaaaaaata aaaatgtcag acccgcaccc acgccgcaga taaaataata ttttacaaat
    26161 tcacgactgc cccacaccat ttccagttct ttgccgaaca tccacagagc cagcatatta
    26221 aacaaaatgt gaaaaggatc attggcgcta tgcaaaaaca tataggtcac aagttgccag
    26281 atttcaccgt cccagatgta gggtgacaag gccagatgca tggataccca tcgccccaat
    26341 ccaaaaaata tcaggatgta ctggataaaa tataccacga cattgatcga aataagaagt
    26401 ttgaccatcg gatagtcggt tcccaaccac cggttataac tgtcaaatcg ggaatgataa
    26461 cgcatgcagt aactttcttt atttaaggga tcggtatttt ttcatacaca ggcaacggga
    26521 taaaacttac aaaaaaaata aggaacgata caataccaat cgcaattcgt ttgttatcca
    26581 gcggttcgtc ttcatccatc acccgcggat gtttaaatcg aatcaaaaat aaaatcagga
    26641 gcgcccagat aaaccagctc cccgccccat agaataaacc gatcgtaatg atcacggcaa
    26701 atgccccata tgccgcaaaa cgttgctttg atccgatcag tgcatataaa atatgaccac
    26761 cgtccaattg gccgatcgga agcatattga gcgcggtgac caataatcca aaccatcctg
    26821 cgaagataaa cggataatgc acgatatcat acatcgccgg catacgatca ccggcgagtc
    26881 cgtggccaag ccatctgaac agcaacgaat caccaaaata catcacaggc tggcccgaaa
    26941 aaatttcttc aacattgtaa aactgatacg cccatgtttt atccgggata gtcgcgaaac
    27001 cgtatatgag cacgaataaa cataccacga atcccgcaat cggtcctgcc gcaccgacat
    27061 ccatgagttg acgtttgtta ataattggcg acttgatacg aataaaagcg ccaaacgtac
    27121 ctggcataat tgttgtccca ggaaaaccca accacggtat cggtggaata agaatccccg
    27181 gaagataata aggcaacgtc acattaagct tatgataacg cgcggcaaaa taatgcccga
    27241 attcatgcgc tgttaatata cctaacagcg aaaaagaaaa ctgaaaaccc atcacccaat
    27301 cccagtcatc ggataatgat ctgttggtta tcgttgcggc acccgtcata aatacgacag
    27361 caaacgtaat aacaaacaag atgccattca cccacggatt gctcagccat gcccagcggc
    27421 cgggcgtttc gctctcttgt gtcatagtgt aacgtcgtat cggattccat gcggccgtta
    27481 ttggttcgat cctgagcacg atggtatctt ccattccgcg cggtatacgg ttaatgctgg
    27541 caatcgaact tttaagcccg agcggcgcta aacgcgcctc gatttgcgcc ataatatccg
    27601 tattgatttg cggtgtgcgc aaagctacgt aggcagtatc ccactcgaaa actatttctt
    27661 cgatagcgat cagatcgccc agcgcggcgc gcaattgttc atccattatc attatccgtg
    27721 attttaaatc ttatttacat tatcatcgca ccgatgcata acctcactcc acagcacatc
    27781 atcttactcg ccggtgaaaa atcgggcgat caatatggcg ctttattagc acagcgactt
    27841 ctgagtatca atccggctat ccgactgtcc ggtatcggcg gtgatacgat gcaaaaagcc
    27901 ggtgtccaac tatgggaaaa taccacgcat cattctgtca tgggcttcgt tgaagtcttt
    27961 cgttcgatca ggtactggtt aaaactgcga aaaaaaataa tccgacttat cctcaccgaa
    28021 aaacctgacg ctattatctt gattgatttt ccggatttca atctatccgt cgcacgctcg
    28081 ataaaaaaaa cgctgggaga acgcagtcct tttatttttt attttattcc accgcaagtt
    28141 tggatttggc gtaaaaaacg cattcacttc attgccaaat tatgccatac cgtgtacccg
    28201 cttttttcgt ttgaaaacga cttatatcaa agccgcggaa tacgaagtcg ctacttcggt
    28261 catccgattc gcgacatgct tagcgccgga aaatatcccc aaaacccaca tcaaaagcaa
    28321 gataaccaca taacgctcgg acttcttccg ggcagtcgca accaagaagt tgttcgcata
    28381 cttccgatca tgttgcaaag cgtccttctt tattcgcaac gatcaaggca accccttcat
    28441 ctgtatataa gtcgcgtcgg cgaagtagat gcgttcatct atgaaacgat ccatcggcgt
    28501 tttccgacat taaatattac atggtgccat accggcgatg aagcgataac acacagtgac
    28561 gccatcttgg ccaaatcggg aaccgtcaat ctcgaactga tctaccatcg taaagcgtcg
    28621 ttgatcgtgt atcgtaccgg cacactcacc tacctgatcg ctcgagtctg gttgcgtatt
    28681 cgtcatattt cgctgatcaa tatcctcgct ggaaataccg tcgtgcgtga atttatacaa
    28741 caccacgccc ggcctgaatc catcgcacaa gaaatcgacc gcctacttca cgatgacgtg
    28801 tatcgccatg acatgctcgg cgaactggac aaactgcaca ctgcattatt tcccgaatcg
    28861 gaatccccat cggctgtaaa tcagatcgcc gcagacatgc tcagtataac ttctcgataa
    28921 cagaaaaata aaagaggctg tttcagccgc tcaacttgat tttttcatgc gacaaaaaaa
    28981 tcttatccgt tgaacttttg aaacagcctc ttaaaagctg aaccgtatta aattttattt
    29041 tttgccttcg ctcaatttgc gtgcttgctc ttcggcggct ttggcttctt ccagtcggcc
    29101 tgtttctttg tacagtgtag cgagcatgac gtgcgcttgc accatatcag gcttcatggc
    29161 gatcaacgct ttgatggccg tttcggcttc tttcatcaaa ccgtaatcgc gatacaatgc
    29221 cgcactcaaa agataccact gcggattggt attatcatta ttacaaactt tggccacttc
    29281 tttgatgtca tcttccaacg tttctttatc ttccggagcc agtaatgaaa acttggctgt
    29341 cggagaaact tcagcgccgg tcgcatcttt gatggaccat tgatattttt catcagactt
    29401 caatttgggc gctgttttag gatacgcggc ttgtttcgaa cctttgatgg tcatttcgta
    29461 tttaaaatca gaaatttcac tcgaaatcgt gatcacatag tcgttgcccg gtgaggcgcg
    29521 gtgatcggcc cattcgaaaa cgggactatc gttgatgact ttggattctg aaggatatac
    29581 cgcagtaatg ttacgattgg catcaccggc ccaggcgcgt gtcgctccgg ccatggacga
    29641 attagaagct tctccccaca acatccccga cagagatttg tacaatcccg aaaggaatga
    29701 atcttccgtt ttgacggccg taatcacgtg attttgtccg gctttcagaa cgacttcttt
    29761 gccgctgtaa tacatgacac tgacggaact gttggcgctg gctgtgatct tatcgccggc
    29821 cagcaatttg agactgggcg atgctttttt ttctttaccc gacgcatcgg ttactttgat
    29881 cgtgcctttg atggatgaca ggaacgcgac accgttatcc tgcccgttaa ccgccgaaag
    29941 gcccaaagcc aatgcgacga accatgttaa cataaatgac ttcatacctc acctctctat
    30001 gttagaatac tcatctcaaa actttgttga actgatgtga atacaaacga aagtcatcag
    30061 gtaaaccgct tcgcgcgaat ataatttaaa acctcttgta aactcaaggc ttatttcgat
    30121 gtcagatggt atgtcccatc ccaatccggc gccggcggtt ctgcgataaa ctgcttacag
    30181 cggtgaatat acagtttgga cggttcatcg gtatgcgcat acgtaagcgc ttgctcaaat
    30241 cggcggatcg cttcgctcca ctcacgtttg tggtacagat caataccttc ctgataacgt
    30301 gctaaaagat ggcgtgtgtt ttccggcaaa ggctcattag cacgacccat aagttcgtac
    30361 actttgaccg gtttggtttt gcctttgacg acgatcatat ccagctcgcg cacgatcacc
    30421 cgctcacgta cctgagcatg ggtaaattcg ctgatcatca gatacgtgcc gtattgttta
    30481 ttcgccgctt cgaggcgcga tgccaggttc atggcatcac cgatgcctgt ataatcgtac
    30541 cgaatatccg aaccgatatt accaaaaact acccggccgg tattcacacc gatacgattg
    30601 cgaatggccg gcataccttt ggccatccat ttagcgcgaa attcttccaa ggaagattgc
    30661 atgtccagcg cagcataaca cgcatgcagc gcatgttcat tttgcggcaa tggcgcacca
    30721 aatacggcca caatcgcatc gccgatatat ttatccaacg taccgccctg ttgaaaaata
    30781 cgttgcgtca tcgcgttaag ataatcgttg agcaattcga ccaattgttt cggcgccatc
    30841 gtctcggaaa tagacgtgaa tccttcgata tccgaaaaca aaatactgat ttctttttct
    30901 tcgccgccaa gctccagttt cgacggatcc agcacaagtt ggtctacata cgccggtggc
    30961 gcgtagcgcg aaaacattcc tttgatctgc tttttatcgt gttcttccgt aaacgcctga
    31021 aatatggatg tcataaaata tgaaagcaga accaccagag acaggcttac ggcgggaatc
    31081 caccatcccc cggcaaacag aatatgcgct ccggtccata cggtgattaa aagaacggtc
    31141 agcgtggcca tttcggcggc aaatcccaaa cgtaccgtag ccatcaccat agtgatactg
    31201 agcaacagca tacacagtgc gatcaccggc cagccgggtt tatcaataaa tgacttatta
    31261 agcagggtga gaaatgcatt ggcatgcact tcggcgccgt acatccaatt gcggcgcgtt
    31321 tgttcgaaaa acggggtgat aaaccgatcg ggtacaaatt ccgaaccgtt tccgacaacg
    31381 atcaatttat ctttgaaaat atcttccggc cattcgatat cgccggtcag ctggctgctg
    31441 cggtaggtcg taatgcggct cggcgtcatt tgcgtccacg taccgtcttc ctgtttcacc
    31501 cattcgctcg gcgcgctgat atagttgatc agcatgttgc cttgggcatc acgcggaata
    31561 cgcggcaaaa gctgccagcg attttcacgt atatcacgga taaattcctc cgccggaaga
    31621 tccatcattt ggcagtaagc cgccgctgaa aaagaaagca acatggtatt gtcggacatg
    31681 gtagccaccg gacgcacttc gcgcacactc gctaaaccgc cgccgccgga aatctgaaga
    31741 ttggcatatc ccacggcttg caaagcggga cggaaaaacg gatccggttc ctgtatgcgt
    31801 atggaaccgt cttcacgttg atgccaaatc gaaaccgaaa tgacattgcg ggcacggtgc
    31861 atcgcatcgc gcaaaagtgt gtcgccccgg gcgtcgagtt gatctaactt atcataaaaa
    31921 gcaatatcga gagcgattgt ttttgcgccc ttggccttga gtgtatcaat caatcgcgca
    31981 agataacgcc gcggcaatgg gtcataatac ccaaagtcct tttcgtacgc cttctcgtcc
    32041 accatcacaa ccacgacggg aatttgttcg ggcacatcgg ttcgtatctt aaacaaaaaa
    32101 tccaaaccgc tttcttccca gctgcgaaat gccggcgcat gcgccacatt gagtccgact
    32161 aatacggcga cgacgacgat catgctgagc cacaattttt tattatggcg cttgacttct
    32221 aacttcatac acaactccgg aaaatgggga acaatttatc cttggatcgg cgcactttcc
    32281 gtgcccagag atacgcccga gtccgccaag cggtgcaggc gttcattgat taatagaata
    32341 gatgtgctat cgctaaaccg catacgaata tggccgaata attttcgacg caactgaaga
    32401 tgcagtacgt tgttttgcca ataaaacgat tttatctcat tccattgaaa ctccatttcc
    32461 gactggacat taacactgcc aataccgatt tcattgaaaa agcaaatgcg tgacacaacc
    32521 gcattgacaa aaaagccgac ggctaatgct atgcatgcga ctagattggt gtgattttca
    32581 gcgtcggata agagaatcag cgcactgata agtaacgtga caaaaaatcc gcaccagtac
    32641 actatggttt ccaaacgccc tgcggtcgcc tgataaaact gtgctttgcc catcgtgcgt
    32701 tcactaaatc ggataaccgc atataattcc tgatagatat tggccaatac cgtaaatacc
    32761 acaagcgcat tgaagatcag caacataaaa gaatgatttt cgaacaaggt cataccgtgc
    32821 cgtaagtttc agaattgata ttgaattgaa aaacgattga gtgtaccgat gccccatgat
    32881 gcaaatgaat aatccacgat atatttatcg tatttaaatc ctataccggc agaggctccg
    32941 gccatacgat ccaaaaaatc cgaagaacct gcatgcaatt cacggtcacg ctgtaagctg
    33001 taaccgagac gtaccgacca actttcatgc agcatccact ctgcgccgac taccgggctt
    33061 ttgaccagat cattttgtat gtctgtaaat gtcagattga ccgtaaccgg ggcattctgc
    33121 agtttataat ttacgccgac ttgcaatgat aacggcaggc tttctttata gctggctgca
    33181 aaagtccgcc cttgtagtgt tgcaaggcct gccggattgt a
//
LOCUS       DAOQGF010000028        32971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_609_length_32971_cov_8.433983, whole genome shotgun sequence.
ACCESSION   DAOQGF010000028 DAOQGF010000000
VERSION     DAOQGF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 32971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32971
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_32971_cov_8.433983"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK937_05880"
     CDS             <1..260
                     /locus_tag="PK937_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HNH32132.1"
                     /translation="ADEIIDISDSSDRDYIQGQDGTLTVNHEYINRSRLRVDARKWKA
                     SKLYPKKYGERVEVENTGNMKFVITTKKRETDTLETNSDAD"
     gene            250..1608
                     /locus_tag="PK937_05885"
     CDS             250..1608
                     /locus_tag="PK937_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HNH32133.1"
                     /translation="MLTEIEIDEDVFLPCYRHLNNDTGIDIDLIYGGRDSGKSYNQAQ
                     RLVIRCLESDYFRYILARKTANTIKDSQWHLIKDVVSEWGLESLFTFNISPLEIRCAN
                     GNKFICRGLDEPAKLKSIANPSGAWVDEGNQITEEDFIFLISSLRSNTGSIKLDFTFN
                     PESDTPDFRDFWIYKYFFSHTSEKSFGNVKTFEAGSTILSLTYRATHTTYLDNPFVTP
                     QRAAIHESFKATNLYWYKVFTLGEWGNRLNESPWAFAFSRERHVRRGLKADKTLPLYL
                     SWDFNRNPVCCSVLHHREGEKKIQVIETIKIPNSGVDEICSRIKVKYPGFLYIVTGDY
                     SGHTPTSLYKEQVTNYTVIKANLNLSDGQIRTKVNPRLEKNRTLFNIVLQYYGFEVDE
                     VNAADAIFDLENVQSGADGSIVKDDRKDPRQQADILDTIRYFVNLVLPDFEKMYVLPE
                     KR"
     gene            1655..2002
                     /locus_tag="PK937_05890"
     CDS             1655..2002
                     /locus_tag="PK937_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32134.1"
                     /translation="MATILNQCEDCVEIVFDECADIPFHPSGGISDGSYWWFITDNFG
                     NTWKQSFTVSEGEATILKALLPEGFTTSAIGQIDIRIRDAEDSTTDISFEYNSIEYTC
                     IKATFSKVTLIPD"
     gene            2004..2366
                     /locus_tag="PK937_05895"
     CDS             2004..2366
                     /locus_tag="PK937_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32135.1"
                     /translation="MNVIDLIVSAAITSLQITFIHILFWDGMILQSLRNNPLPWLVKK
                     PLYDCLICMTSIWGIFIWFFEWDGKFDLIAFLFLVGGINVLIMALVNNADDKNGEYFV
                     EMNRQKRITSDYHKIFRN"
     gene            2593..3234
                     /locus_tag="PK937_05900"
     CDS             2593..3234
                     /locus_tag="PK937_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32136.1"
                     /translation="MFRSRSSFNKEGLIVSVAFVSGGREYFSLDTIYNLPYQRGLAAV
                     RVYEEMKMKCDYDFLKAHCEAVNNIFTESKKLGFEEMTKIKRLNDYLIERLNIAVDPD
                     MMYKLAAVVFFDKTESPFIYDSEYAKKKIAHWKKHDDVNDFFLQQPLQKLIPFLRSPE
                     INLQTYSEVMEKLNRIHWEEVLHLLSLKQKQQYSDMPGSFAGAMPLRSEVLTD"
     gene            3313..5265
                     /locus_tag="PK937_05905"
     CDS             3313..5265
                     /locus_tag="PK937_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32137.1"
                     /translation="MPDQIKIEFIADTTGLKPAVDALESMGAIDKTIADDFRKNNAAI
                     SERIKLLDATGDSAEKLAGDFKKVSDAVKVEGGKNAVDELATGLNQATDKSQRLTTQL
                     RAMKQELSRMDQAGQSGTKAFNDLARAAGKLEDQIGDTAKRIRALASDTKNIDGVVGV
                     VSGLAGAFSAAQGAAALFGGENEDLQKALLKVNAAMAIATGVQQVANTLQKESAAAIT
                     ITTAAQEAYAFVVGTSTGALKIFRIALAATGIGLFAIAIGAVVENFDKIKAKMVELFP
                     ILGHFGDVWIGIKAIFFGTIAAFTEGITGVGKAIKLLFSDGFGAAKDALVSIDLKGAF
                     KKEVEATYAEAKAATEKSSDELVKTIKTGGDKVITARKEVAKREQEEIKALPINNPVL
                     AAEDQQLVEKAKTGEAFKGAQERIDIANTEQSEKNALNDIEMATAANLNNFIISLGDN
                     RIAAIEAQAKRGIITEEKAAREIARIKRAQAIASKGEALFNIAISQPQAIAKTLGLLG
                     IAGAAATPIILALMALQIGSVLAQPIPKFAKGTKNAPPGFKLVGEAGPELIYDDGGYP
                     IIPNGDTQKILNKWMPGTPSIPESVLSGMETKGYGIDYTLLAQSLAKEISKLPISETK
                     FDENGVTKFIHTGMSRREIKTKRANF"
     gene            5266..7665
                     /locus_tag="PK937_05910"
     CDS             5266..7665
                     /locus_tag="PK937_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32138.1"
                     /translation="MDWTFIYTCSNDSATLTSADEPIDFIDYTITMRRDPKTHGKMIE
                     LSNSLTFFGDAYAFIKDKYDTFGVEADIVLSISFDCDGSSDSLPDSRIDMTSLMFEEG
                     GSCTVTADLEEATCIMKFKNRQDLKVDILSLSTIDDPDGETLTPYDGIGISTTIPSKA
                     IQKVDELFYGSDDSVEMATTTYQDDFTEDAEGYIETEGYLWIRQGDEHGEIGGLNSDF
                     TTNRNSGATALDIWTNTYVQYADPNIYFAGLYSVDLEIAAVLDNATLDGGGAGDKFDR
                     VQVDLIARQTVPGPSSSETIFQSWDFNSGPYDNTFSENLVCDLTAAWQIAFLESSLPD
                     ADTEGNSYSFYFHVTVSGTYSKINPMANMGFSFSTTITDRSESYVRWYVMTRFPESIS
                     KTSRINESISRVAEIITGDCLRMYSSCFTRNDAQPEPAELPEDDPDYVFPTNGCNSTY
                     VIMNGLMLRHIPDAKCFVSFRELYDSLNAIFCLGMGIEDDPYRPGYQRIRIEEADFFY
                     EDTPMSYGINYPDKIKRSIKSDQFYTDLTIGYQKWEAEAFSSLDEFITRRQYTTSLTQ
                     IGGNKSLLSSIIASPYAIEITRQQKLTTKDYKYDNDLFIIDPNSDDSLEGGSDVIDYV
                     SYYNVLLSPLRNATRWFKWLFSIFKNHLDADSKLIFCSGEGNYDAVLVYPFNTCDPVD
                     GVVAVETTEDMDLTYPIKFDYENNPPKVGFEQWDFTYPMSFENFQEVRNNFYQQLPVQ
                     WDDSGVKYGYVMEMAYKPAQGLATFKVLRAWEDSATHYLLLEDGTYILQEDGGYIIWE
                     P"
     gene            7678..8709
                     /locus_tag="PK937_05915"
     CDS             7678..8709
                     /locus_tag="PK937_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32139.1"
                     /translation="MANKKISELTALTTPDDADVFVLVDTSATETKKMTWANIVAAIE
                     SGLNAASETQSGIAELATTTETRDGGDDSRIVTPAKLLDWWSNYILQNGVEFSSYITA
                     IEAISTEDDLYLKSVGSVYLRLTGTPTAYRVITLPDATDTLVGKATTDTLTNKKLSDS
                     TVVFGNVSDITKALQFSLGGATTGKKLTVVSSHTDNRTATFPDRTGNITICSPTKDAD
                     ISGVSTITFTGTTPPSTLTGRYSWDYISNSIIQCNVRLEWNSAGVGLTAIEFTLPSDM
                     PTPSFFTGWGNSEFGPPANAEFYTSVTGTPVATRGYTAKSAGGALVIGGTCSSNALIG
                     ASFNFQYFI"
     gene            8878..9852
                     /locus_tag="PK937_05920"
     CDS             8878..9852
                     /locus_tag="PK937_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32140.1"
                     /translation="MKLFTTCDTSDLVEVPAYIYGTDEEVFATIAWTHMTDEGIAYGF
                     MTQTNDDFDFGQVFENGDCFRIGIVRVNSDSYKYANFFEVTGSDVTIKLTINGVEQTL
                     GIFDYSTPDDIADAIRSYITDQVTVLQAVNTGYVSITIHSLSGDTYGNFKLGGTTYTA
                     DVTAGEDDIVACSNCFVYKADPCFTTVLKYRNSENSFGFNYEVDEEFYNKVRVGMYID
                     NPQPVDSENVYRQSNGVYKTLSAYFESVSEGHVEYYDRQRHFAIAMAMRHDDFSVAQG
                     NETEFTSYVRSGAYEIGWLNKPGTNIDSAPASFKVKETPFNYINSNCV"
     gene            9849..10724
                     /locus_tag="PK937_05925"
     CDS             9849..10724
                     /locus_tag="PK937_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32141.1"
                     /translation="MTTDKGILIIACGHPFYGRMALNLAASIKSVEPGMNIALRHAGS
                     AVKYIDQSQMNVFDDVALIDESIYLTDKSKNNWIRTKLFLYDLSPFDKTIFIDADTLW
                     LPKRKPSDLFDDLKDITLTFANYKRHDFNTVDDKERVWANIKEVKDVYKLDSGYYYCL
                     QSEMIYFTRTDDNRTFFEMAKIVYDNPKVKTTIFAGFVPDELAFSITMAKMGLHPHVT
                     PYYPTYWHPVQKHVNRDNLFDRHYLLSVGGAFQTDYVKDLYNTLASVAFRKLQMETPW
                     KYFNKRQWLPERKTI"
     gene            10726..>12077
                     /locus_tag="PK937_05930"
     CDS             10726..>12077
                     /locus_tag="PK937_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32142.1"
                     /translation="MPTILTPELLKGYFKDGKRGAIYEQCISLEKGLRIHVDGIMPED
                     IIGRQRPSEPKIVMDYRKQIWQAITKETVSRVITCLSKIRRSQDWSVRYDEKTFSAKI
                     AKNETPKEYFEKKFPYFSSLTNWLFSVCLKEYLIDANAVCVVMPLDFTPMDPTEYLQP
                     YPYIFNSGQVYDYVVNDYAVIRSKDTATYMTDDGKAKRTDGAIFWVITTTHILRYEQV
                     NEKGDLAIKINYEHSLGYMPAFKLGGMYLKAVDKVPVWESRINSIVPRLNKAAREDND
                     LDISVVRNLFLEKWEYVSQECNKCNGLGKVNGSSGFTTCEACNGQGKIVSSPMQVHAI
                     KPASLNEQPLPTPPAGYIEKPVEIIRIAEERIEKHLYKALAAINMEFLAKTPMNESGL
                     AKQVDKDELNNFVYSIAEDIVFIIDNVYKAGYDYRYKVVVPIDSERYAMLPVVNVPEK
                     "
     assembly_gap    12078..12177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12322..13221
                     /locus_tag="PK937_05935"
     CDS             12322..13221
                     /locus_tag="PK937_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32143.1"
                     /translation="MDDKIIEQLITTIDDSITSFTGAIPDIQRQIFDDINLLVKDLDI
                     TSDGTIKNNVANLKIIGRIRGKLEEIILSPEYMKKVEDFVKTFNEVADLQNKYFRSIE
                     GKFKPTKLLDEISKQAIGWTVDGLTASGIGANVTDEIEGILRTNITTGGSYKDLANQL
                     RDSILSNKSGQGLLERYVRQVTTDSIMQYNRQYSHSISEDIGLQWYMYTGSNINTTRA
                     FCIACTEKKYFHKSELKPLVNGDLFSRKDVPVNPKTGLWYGAIEGTNASNVLINAGGY
                     QCGHQFQPVLSSAVPESVKAKFQ"
     gene            complement(13479..13943)
                     /locus_tag="PK937_05940"
     CDS             complement(13479..13943)
                     /locus_tag="PK937_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32144.1"
                     /translation="MKRLSDDEVLNDFIEYMSLNGQNFYSLDDYVKYKLKQPIKRNLH
                     DLLSITGWVDLFQGFGNEPNKYYISPLGFNGLKRYGSYLNYLKEIKKSESLQMDKLWY
                     EVNLLKREFEDYPETKKQASDSLRYSKISILVAILIPLLILMLQWTCNKHVQ"
     gene            14134..14637
                     /locus_tag="PK937_05945"
     CDS             14134..14637
                     /locus_tag="PK937_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32145.1"
                     /translation="MPIATINFEGEVYPFFQSQGFASQYAFPFAKQVCIGTGFDIGCG
                     KKEWSLPDSIPIDKNLPDEWDAMNLPPFMVDFIFSSHCAEHILDFVKTFDYWTSRIKS
                     GGVLFLYLPHYSQRYWRPWNNKKHYNIFTPEILRDYLQQAGYNTNKIFVSEVDLYNSF
                     MVMAEKI"
     gene            14641..15198
                     /locus_tag="PK937_05950"
     CDS             14641..15198
                     /locus_tag="PK937_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HNH32146.1"
                     /translation="MNQIWKEISGYEGLYEVSNDGRVRSLDRWVTHPYGMALKRGRIL
                     AAATIWSGYKRVLLIKDSIRKNITVHRLVALAFVENPNNETQVNHIDGNKENNSFLNL
                     EWCSIGYNNRHARNTGLNVARFGKESHSYGFKNKRSHALRNIETNEIKPIVEVAKEYP
                     QWSRRYITMMIMGGRGNKTKYVLHI"
     gene            15195..15956
                     /locus_tag="PK937_05955"
     CDS             15195..15956
                     /locus_tag="PK937_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32147.1"
                     /translation="MTIAFCTYVSDKYYHSFGADKLLASAKYFHPDIPFFVFGDKEIA
                     KFPLPVESLHPFMMNKLMAKFDIVVHIDADSIICGDITPVFEALETADVVCVRNNNDY
                     GKAGCDKALSQQNRDINIYVNAGFVATKSKEFVVDWMHNNLLFAELTPFGSQSVLNTI
                     IDKYHWKLIDGIDDDCYYGVSCLHGENSHWESWQRIQVDENGNLYLENYERAKTVKVL
                     HHAGGFSPDKLGLYLFNDETRNRLTEICYGKKIGD"
     gene            16167..18260
                     /locus_tag="PK937_05960"
     CDS             16167..18260
                     /locus_tag="PK937_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNH32148.1"
                     /translation="MKLGIVIPVYNRPEYLRQCLDSVIRSILPEGINIVVVNDCSTHP
                     DMNRILAGFAENLRGKSITFKNNRVNSGVAYSIKAGIATLLEAGCDMFINLDSDAIVR
                     REWIDKIIELKNLFPSKIVTGFNCLTKNDNGTERHPITEQAQGYNFKKSVGGLNMCFD
                     RTQYNSCILPALEKAIRRECNWDAEACKYSGGAACVVPSVVQHIGINSSMGHHETPDT
                     ADDFVKDEILPEVAKNGFITGDISEEKRQGNTKLSLPSVTLVTVNCDAVEAGIFAMNK
                     SMEGIDFGSVKFLTSQDTDYEYAVKIPHIASIKEYSRFMVYELYKYIDTEFILVIQSD
                     GYVMNPAAWTDDFLKYDYIGATWWFRDRHNVGNGGFSLRSKKLMEIVATDKHIRIYHP
                     EDEAICRKYGRFLQVKHGIEFAPDNVAKKFSIEGWRQNDKTYIGQFGFHGRGVIFPGT
                     LSGSGTTQIKQPERKPDIIVINQPFGLGDFLFCIPLMRDYINEGLKVIVPVIGQYDNI
                     NKHFHDILFINKEMLKINYERKDEYTISGMKVIPLRWSYEILKVPFSDCMKSKYMMFG
                     KDWTRWRECTWMRDMEAEDELYYKVLQLKDGEQYNLINTTFRNDLSGEAKVNVNNGFK
                     NVKMRTVPGFTLIDWGKVIENATTIHTVSTSINYMIELMALKAKEIHLYVRKPDEKDF
                     RNINYLFTKHYHLHL"
     gene            complement(18232..18384)
                     /locus_tag="PK937_05965"
     CDS             complement(18232..18384)
                     /locus_tag="PK937_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32149.1"
                     /translation="MKTVITMDGIFDGMEDGEENLPESIESELTKEVKKLLEQSEIKD
                     EDDSVS"
     gene            complement(18450..18707)
                     /locus_tag="PK937_05970"
     CDS             complement(18450..18707)
                     /locus_tag="PK937_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32150.1"
                     /translation="MKNFSDYTNYFKQDHYYEDGTPKDFIGMLYFILFDNLLSKIFLL
                     ALGVVSGMLFLLCFADLIIFIFKGKWFFEFGKNSCSDSGDY"
     gene            19021..19920
                     /locus_tag="PK937_05975"
     CDS             19021..19920
                     /locus_tag="PK937_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32151.1"
                     /translation="MKVSEFIQKLAAKSGINLADEKNKDIATSIASITTELPDESVSK
                     MLGSLMSVDDAKNNSDLKKYFGAQIFDGMDSEFSSIMEEMGFQVSDREAIKATDSAFK
                     KIGAIGRKIKELEEKKSESKGKDKSELQEKINTLTSQLSQLTKDHDNKIKELQSTYEK
                     KLTDSQVRSILKGKKYANDQVPQDVNVELALVLLNKALNENKAVYVNQNGSLVLKQSQ
                     DNTLDWMNSSNEKPSFEQYIDGVLAASKLLAVTPTAAAGNSGAGNNGAATIITGGAQN
                     GANASVMNAFDQTIRELESAPLI"
     gene            19987..21024
                     /locus_tag="PK937_05980"
     CDS             19987..21024
                     /locus_tag="PK937_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32152.1"
                     /translation="MKDLAGINYPGTKITAPGFLKMLLQNSDIMGAKLTNEQGHQKTA
                     TVKYKQRISPSMVATNDNCDVDFSPAFREASLTAVNTAKWGAHISLDTVSRYMEEASR
                     GVTIGGVSVIQEVADTVTQVANALIGKVDRVLLDAVDWGKNVVYGDDFVHDLNFESDA
                     TLFDVGTGFPKLLNDAFENEFSGDLLICGNGIFNAVELSKMASQAALNGIDTSRFTGY
                     KWYADLWSKNSTNWGGNDIGVFSKGSIHFVDFQKYVGFRAGKLGTSVFFQIPIDVDGV
                     TMLFDAQLRELDCPTSIPNIYGTSTSYDRGYVLYLTKTYGLFQVPSDAFATDDRLTGV
                     NGAIHYNVTNS"
     gene            21075..21148
                     /locus_tag="PK937_05985"
     tRNA            21075..21148
                     /locus_tag="PK937_05985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21109..21111,aa:Met,seq:cat)
     gene            21162..22184
                     /locus_tag="PK937_05990"
     CDS             21162..22184
                     /locus_tag="PK937_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32153.1"
                     /translation="MECLRDFVGIEGCDAPEPLSGFTVNDLPGITSFMVGKIASNEQV
                     TYLGAWNDIQERALKKFTSAIISAFHNDKKQRIKSVTQTVNLGRRIDAASVMAASSQY
                     RGVTIELAANGQSAMLQSSLQVIYIQQVSIYATQVYSDFEIKVYDLDSDTVIDTITSD
                     LVEGWNIVRVATAYDATRIFICYDAADFDSVELLIDRTVASDCQMCADAVYGYGNCKA
                     AIYGAASASLSDMTIDKADNTYGLSPVFSIQCRYDWVVCNNIDLFTLAWQYCLGAEVM
                     TERIYSDRQNRYTGIDLEKAKALRDEFQKGFEVELKNAISGIDIDESDCCIECVSVIQ
                     KNYSIP"
     gene            22181..22627
                     /locus_tag="PK937_05995"
     CDS             22181..22627
                     /locus_tag="PK937_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32154.1"
                     /translation="MIAVKIDTQNFASPDKLSRFISADGQADFLRAVCLSMLPVVHDR
                     IHVNGKAADGSPIGTYSDLYLKYTRPKNNRGTDPKVILSLTRQMENDFSVVADGSRYG
                     LGFKNEVNAQKSEWAEQTYKKKIYSLTNEEKEQAVNAANEYVNGVL"
     gene            22614..23219
                     /locus_tag="PK937_06000"
     CDS             22614..23219
                     /locus_tag="PK937_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32155.1"
                     /translation="MAYYKEIVDVIDDAIVAGALTDSRFQNRAFAGLCFLVPCKINDT
                     NVSAMIPAKVSTVGECNFIVPDDTFNLVTYHRVLSTIYRPSISQYGDGTDYIDATADM
                     VLTVISFTDKLRIQPENVEALFISGFPTSFSSALKESLQLRSLNAIITASSFDQVAIF
                     NSEYRGETYFLKPGTVIFQIRYQIIQSFKKDCFTICCEAEA"
     gene            23255..23797
                     /locus_tag="PK937_06005"
     CDS             23255..23797
                     /locus_tag="PK937_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32156.1"
                     /translation="MAIYYQDTCTDESVEHFCDDCEAKESARIRSVFFVKEGYEFSDP
                     TDKTEWQTAAAAGNVVVVPKTRGQFDGGSPNYGPGFGDEEQRYINSTFKLNYFDPNLK
                     NNYEFYEAKKRSKTWRVGYRTESLVWISDKPCTIAPKAAVSDNINEGVEWEVEVTWNS
                     ANNPEPFNMPDDIFTCFLVS"
     gene            24039..25397
                     /locus_tag="PK937_06010"
     CDS             24039..25397
                     /locus_tag="PK937_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32157.1"
                     /translation="MSEITITTSDTPLRDFFASNSSTRMFFEKRGNDIVCKDPLTHEW
                     FDRYVNEGGFLCISHGNGAFSNYYGIDPRPQYLGGGMGWDAVLAPPVNCSILKGDLCT
                     PYTLNGQTFDFFWAHNALRNRMGASSYIIANILQGTIEQVYLQIDQLDQNIIYIQFGE
                     ETTDLWTSGVEYANKCYQWADALREHYPGQRFYFMFDCPMLWKKDVGTSAKAMIWANE
                     IASVRPVDKSLFGIRQYCHLMYFINANDDTDHNIAEVENAITNLLPQYAAAIESSPFA
                     GCKVFIGQISANEGAYTPEVNKGVQYFVNAYVRMMKFFTDNLRDDLTRFIGVCYIGTN
                     SWIDKSLNTNLDFQYVGILNQIFGGGQKALYINQFDPMVDVIGTMKNGVYKIVMQNRK
                     GEPVKLPETITLDGIAVPLSSANVHARKCAARDSTTSTDYNPLQDLTLSGYSIQCFQI
                     KP"
     gene            25403..25675
                     /locus_tag="PK937_06015"
     CDS             25403..25675
                     /locus_tag="PK937_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32158.1"
                     /translation="MTTKILTREESALVEAGRRQTISPLGDLWVALKCYGNDDAFNEM
                     AVQYNSIEDNEIDAVIDAGGTVMETYGNGDVKQISKAPNRPKPPVT"
     gene            25714..26070
                     /locus_tag="PK937_06020"
     CDS             25714..26070
                     /locus_tag="PK937_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32159.1"
                     /translation="MVHFRNLLIIFVTIMAVMWTYMAMADGTATADRYITVDQDIWSF
                     LKYVGVMFLGILSWAAVQLISSLKSLVSQVTDLNITVKVTQNEIMLIKEDVSEINSKI
                     EKQDYRILQLEKAQKN"
     gene            complement(26453..26830)
                     /locus_tag="PK937_06025"
     CDS             complement(26453..26830)
                     /locus_tag="PK937_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32160.1"
                     /translation="MKKTILLIILVVSCFSVLAQSNPSSTTIYYASNGHTFKPGDELH
                     LGIGSDAMKDFIFVYTNPVSLTGKINLGSAYAGATFHIKSIKTIENKTMGKKTYLICA
                     GGNIVNYYCDIEPALATGEVVIK"
     gene            26921..27175
                     /locus_tag="PK937_06030"
     CDS             26921..27175
                     /locus_tag="PK937_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32161.1"
                     /translation="MNSENKKVKVPISDKLFLRWKQLHEHGDFQKMAREYNIPVHKFQ
                     RAFSSRKAEVSVVRSINIFYNRRERLQDAGLKSLSNIEMT"
     gene            27368..27664
                     /locus_tag="PK937_06035"
     CDS             27368..27664
                     /locus_tag="PK937_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32162.1"
                     /translation="MTKKAEELQNLIDPTTPGQVAEPFEFPMACIYLDDRCLILSEKK
                     SVRIENYTHSNTAPFEYVKRKYAEELDVMELLNELEGICRSIRAKMFLKTTSEN"
     gene            27680..28393
                     /locus_tag="PK937_06040"
     CDS             27680..28393
                     /locus_tag="PK937_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32163.1"
                     /translation="MTNRGGDKPKCKDKSTIFINYKNQNKMITATNTGGGDHTPVAAG
                     TYPARCFSMIHIGTIEENIMGETKWLNKVRITWELPTETKVFNEEKGEQPFVISKEFT
                     LSMNEKANLRKFLEGWRGKGFSDDEAKAFDITALLGKPCMLSVIHKTSKKGNLFAEIS
                     SCSAMPKGFTCPPQINDTLELNYTDHWSNIEFSKLPQFIKDKMTGSKEFRAMMNPQEV
                     EAPSETENLSHQTADDLPF"
     gene            28407..28811
                     /locus_tag="PK937_06045"
     CDS             28407..28811
                     /locus_tag="PK937_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32164.1"
                     /translation="MTPSLASTTWDDYHRPPAPKRIEPETPKAIDVIHFKATKENIEI
                     TYYLEGILKEQFKSVDIDTLRQWAIDDKNLLEVYELPCYNSDNDEEYTVIYPATSETG
                     TAEQINQARLDAFIKGLTAESIEIMLEKHLHI"
     gene            28814..29236
                     /locus_tag="PK937_06050"
     CDS             28814..29236
                     /locus_tag="PK937_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32165.1"
                     /translation="MAADLTYFAKVDENGVIQGLARKKFAREVKDMFSGKSIEITVRR
                     KKKHRSVQQNRYYWLVVTMLSDYTGFTKDEMHAILRSKFLRAEKVNEDTGVVYEYVKS
                     TTELTTVEYEEYLDDVRRFAAQEFNMNVPMPNEQIEVF"
     gene            complement(29628..30011)
                     /locus_tag="PK937_06055"
     CDS             complement(29628..30011)
                     /locus_tag="PK937_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32166.1"
                     /translation="MSRQISLFGIRFNRAPVTLFVFLFHLTIKATLYNPVVSFLDGYY
                     QPFDGFKVCLYASLVFGRENDFAVSMLDVCEALADHTARFVDAESNLFLCHLFIYLVH
                     EILFESGSVLLYPSILNSVPFLLQV"
     gene            30130..30573
                     /locus_tag="PK937_06060"
     CDS             30130..30573
                     /locus_tag="PK937_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32167.1"
                     /translation="MDKKQQPKPSYLIGIDPGINNGIAYLDTNTGKLIAVTSCNLHTL
                     FNLVLSELDKPIFVYIENPNTWVPFKNVSMEVMEMRRSGAGAVKQTYRHIIEFLEDHG
                     IEYRPTRLQGSMKKVSSEWFKKFTGWAGKTNQHGRDAAMLVYGRM"
     gene            30835..31086
                     /locus_tag="PK937_06065"
     CDS             30835..31086
                     /locus_tag="PK937_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HNH32168.1"
                     /translation="MTHALKTINPFYKAVADREKTFEVRKADRPFSVGDKLLLQEYSE
                     QGGYSGQEEEFRITYILDGGQFGIEKGFCVLGIKEIEKY"
     gene            31058..31417
                     /locus_tag="PK937_06070"
     CDS             31058..31417
                     /locus_tag="PK937_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32169.1"
                     /translation="MELKKLKNIKMRVGKSFNSLLKNMQTDSLKDKTPTIGNTFLYAV
                     KNGQLKRWFYEFRHGHPFLLSDEADIIVETRLNDKANQWMNDEQIKELVKLRVKAMYK
                     KKFPNAKRWSLPIFNCI"
     gene            31555..31863
                     /locus_tag="PK937_06075"
     CDS             31555..31863
                     /locus_tag="PK937_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32170.1"
                     /translation="MKLNLISTKEILELEKEIGYELEVNERPTHSGLNRFYVSFKNGE
                     SMEGGCLVSHCGNGNTIDEALQDYCKQISCRRMAFGAYTNERKEITFPKLVHTKMLNQ
                     "
     gene            32000..32236
                     /locus_tag="PK937_06080"
     CDS             32000..32236
                     /locus_tag="PK937_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32171.1"
                     /translation="MKLTKRQLLTAQQAVGYAVMYAKTESETAEFALLKLDIDGALIR
                     LEAEEQRKAEHDKMGCPFHYCDSNPPCKGKCHYA"
     gene            32405..32707
                     /locus_tag="PK937_06085"
     CDS             32405..32707
                     /locus_tag="PK937_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32172.1"
                     /translation="MKPVEFKHQNIVFAKDQPEYQPLPALRIDSPTGEVISCWKLSFK
                     ERLKIVFTGRVWLSLMSFNKPLTPSYLAVNRKEVYSHPDDSVTLFSKLKMIFRRRF"
BASE COUNT         9971 a         6820 c         7712 g         8368 t
ORIGIN      
        1 tagccgatga aattatagat atatcggata gcagcgaccg tgattacatt caaggtcagg
       61 acggcacatt aacggtaaat catgagtaca tcaatcgctc gcgcttgcgt gtagatgccc
      121 gcaagtggaa ggcgtctaag ctatatccaa agaaatacgg tgagcgtgtg gaggtggaaa
      181 acaccggaaa catgaagttt gtcatcacca ctaaaaagcg cgaaacggat actctggaaa
      241 caaattccga tgctgactga aatcgagatt gacgaggacg tatttctgcc ttgttaccgt
      301 catttgaaca atgataccgg aattgatata gacctgatct atggaggcag ggattcaggc
      361 aagtcataca accaggcgca gcggcttgta atcaggtgcc tcgaatcaga ttacttccgg
      421 tacatacttg cccggaagac agcaaacacg atcaaggata gccagtggca tttgataaaa
      481 gacgtggtga gtgaatgggg gcttgaatcg cttttcacgt tcaatatttc acctcttgaa
      541 atccggtgcg ccaatggaaa caagttcata tgcagagggc ttgacgagcc ggcaaagctg
      601 aaatctattg ccaacccatc cggggcatgg gtggatgagg gcaaccagat aacggaagaa
      661 gatttcatct ttctcatatc ttcccttcgt tcaaatacgg gaagcattaa acttgacttc
      721 accttcaacc cggaatcaga cacgccggac ttccgggact tctggattta caaatatttc
      781 ttttctcata cttccgaaaa gtcattcgga aacgtaaaaa cattcgaggc gggaagcact
      841 attttatcgc tgacctaccg ggcgacacac accacatact tagacaatcc atttgtaacg
      901 ccgcaacgtg ccgccatcca tgaatcgttc aaggcaacga atttgtattg gtacaaggta
      961 ttcacccttg gcgaatgggg caaccggctg aatgagtcgc cgtgggcatt cgctttcagc
     1021 cgtgaaaggc acgtaaggcg cggattgaaa gctgacaaaa cgcttccgct ttacctttcc
     1081 tgggacttca accgtaaccc ggtttgttgt tcagttttgc atcaccgcga aggggagaaa
     1141 aaaattcagg tgattgaaac gataaagata cccaattccg gcgtggatga aatttgcagc
     1201 cggataaaag tcaagtatcc cggatttcta tacatcgtta ccggcgacta ttcgggccat
     1261 acaccgacgt ctttatacaa agaacaagta acgaattaca ccgttataaa ggcaaacctg
     1321 aacctatctg acggacagat caggacgaag gttaacccgc gacttgaaaa gaaccggacg
     1381 ctgttcaaca ttgtgcttca gtactacgga ttcgaggttg atgaggtgaa cgctgccgat
     1441 gctatttttg acttagaaaa cgtgcaatcc ggcgctgatg gatccattgt gaaggatgac
     1501 cgtaaggacc cgcgacaaca ggcagacata ctcgacacga tacgctattt cgttaatctt
     1561 gtgctgcctg actttgagaa aatgtatgtg ctgcctgaaa agaggtgaat atttttttta
     1621 ttaaatagtt actaaaaaag ttatttttgt tttcatggca acaatactta accagtgcga
     1681 agattgtgta gagatcgttt tcgacgaatg cgctgatatt ccatttcatc catctggcgg
     1741 catatctgac ggtagctact ggtggttcat cactgacaat ttcggcaata cttggaagca
     1801 gtcgtttact gtcagcgaag gagaggcaac gatattgaaa gcattacttc cggagggatt
     1861 cactacgtcg gccattggtc agattgacat caggataagg gatgccgaag attcaacaac
     1921 tgatatttcc ttcgagtaca attcgattga atatacctgc atcaaagcga ctttttctaa
     1981 agtaacatta ataccagact gatatgaacg tcatagacct catcgtatcc gctgccatca
     2041 catcactaca gatcacattc attcacattc ttttttggga tgggatgatt ttacagtcac
     2101 ttcgtaacaa tccgcttccg tggcttgtga aaaaaccgtt gtatgactgt ctgatctgca
     2161 tgacgtcgat atggggcata ttcatatggt tctttgaatg ggatgggaaa tttgatctga
     2221 tagcatttct ttttttggtc ggaggcatta acgttcttat catggcgctt gtcaacaatg
     2281 ccgatgacaa aaatggcgaa tacttcgttg agatgaatag gcagaaaagg ataacttctg
     2341 attaccacaa aattttcaga aactgatgtt tgactttctc actgaaaatg gattctctta
     2401 caccggcatg tgtgccgtgt gtcataataa ttttaaggtt tacacgaagg gcaacacgga
     2461 gataaaagct cacgtttccg gcaactactt taaattttat gagcctgaca agaaaggaaa
     2521 catgaggcag acggcttacg gaaacttcga caagctaaaa acattacttg cgtgataaca
     2581 tttttaaaaa atatgtttcg cagccggtca tccttcaaca aggaagggct tatcgtatcc
     2641 gtagcctttg tatccggcgg cagggaatat ttctctcttg acacgatcta caacctgcca
     2701 tatcagcgcg gactggctgc cgtaagggtt tatgaggaaa tgaaaatgaa gtgtgattat
     2761 gattttctca aagcccattg tgaggcggta aataacatct tcacggaatc gaagaagtta
     2821 ggcttcgagg agatgacgaa gatcaagcgg cttaacgatt atctgattga acggctaaac
     2881 atagccgttg atcctgacat gatgtataag ctcgcggcgg tggtattctt cgacaagact
     2941 gaaagccctt tcatctatga cagcgagtac gcgaagaaga aaatagcgca ctggaagaag
     3001 catgatgacg tgaatgattt ttttttgcaa cagccgttac agaaattgat tccattttta
     3061 agatcgccag aaatcaattt acagacctat tcagaggtga tggaaaagtt aaacagaata
     3121 cactgggaag aagtgttgca tctgctctca ctgaagcaga agcagcaata ttccgatatg
     3181 ccaggctcct ttgcgggggc gatgccacta aggtcggagg tcttgaccga ttaagtttcc
     3241 ttgactatct gtttcatctt tcagagatga tgcgggagca gcaagaacta caggcgcaac
     3301 aatcaaagca taatgccgga tcaaataaag atagagttca tagctgatac tactggactt
     3361 aagcccgccg ttgacgcatt ggaatcaatg ggagccattg acaaaactat tgccgatgac
     3421 ttcaggaaga acaacgccgc tatttctgaa cgtatcaagc tactcgatgc taccggcgat
     3481 tcagcagaga aactggccgg tgatttcaaa aaggtttcag atgctgtaaa ggtggaaggt
     3541 gggaagaacg ccgttgatga acttgctacc ggtcttaatc aggctacgga taagtcgcaa
     3601 agacttacta ctcaacttcg tgccatgaag caggaacttt ccagaatgga tcaggccggc
     3661 cagtcgggaa cgaaggcttt taatgacctc gcgagggcgg caggaaagtt agaagatcaa
     3721 ataggcgaca cggcaaagcg catccgggcg ctcgcctcag acacaaaaaa tattgatggc
     3781 gttgtcggag tggtatcagg ccttgccgga gcgttcagcg cggcgcaggg cgcggcggca
     3841 ttattcggcg gcgagaacga ggacttacaa aaggcgttat taaaggtcaa tgccgctatg
     3901 gccattgcta caggcgttca gcaggtggcc aacactttac agaaagaaag cgccgcggct
     3961 ataaccatca cgacggcggc gcaagaggca tatgcctttg tggtaggcac atctaccggc
     4021 gcactaaaga tattcaggat agcccttgcc gcgaccggga tagggctatt tgccatcgcc
     4081 attggcgcgg tggtggagaa cttcgacaag atcaaagcga agatggtaga actgttcccg
     4141 atacttggcc acttcggcga tgtgtggata ggcatcaagg ctatattttt cggaacgatt
     4201 gctgccttta cggaaggcat caccggcgta ggcaaagcga tcaagctact tttttctgat
     4261 ggcttcgggg cggccaagga cgcgcttgtc agcattgacc tgaaaggggc ttttaagaaa
     4321 gaggtagaag cgacctatgc agaggcgaag gcggcgacgg agaaaagcag cgatgaactg
     4381 gtgaaaacaa ttaagacggg aggggacaag gtcatcacgg cacgtaagga agtagcgaag
     4441 cgcgagcagg aggagatcaa ggcactgcct attaataacc cggtactggc cgccgaagat
     4501 cagcagttag tagagaaagc caagacaggc gaggcgttca agggggcgca ggagcgtatt
     4561 gacatcgcca atacggagca gagcgaaaag aacgcgctca acgacataga gatggctacg
     4621 gcggcaaacc ttaacaactt catcatctca cttggcgaca atcgtatcgc cgccatcgaa
     4681 gcgcaggcaa agcgtggcat cataacagaa gaaaaggcgg cgcgggaaat agcaagaata
     4741 aaacgagcgc aggccatcgc aagcaaaggg gaagcgttgt tcaatattgc catatcgcag
     4801 ccgcaggcca tcgccaagac gttagggctg ctgggtattg ccggagcggc agcaacgccg
     4861 ataatacttg cactgatggc gctacagata ggttcagtcc ttgcccaacc catcccgaag
     4921 ttcgccaaag gtactaagaa cgcgccaccc gggttcaagt tggtaggcga ggcagggccg
     4981 gagttgatat acgatgacgg cggctatccg atcatcccga atggcgacac gcagaagatt
     5041 cttaacaaat ggatgccggg aacgccttcg ataccagaat cagtcctttc aggcatggag
     5101 acgaaaggtt acggcattga ttacacgcta ctggcgcaat ccctggcgaa agagatttca
     5161 aagctgccta tttcggaaac aaagttcgac gagaacggag taaccaagtt catccatacc
     5221 ggaatgtccc ggcgtgagat aaaaaccaag agggcgaatt tctaaatgga ttggactttc
     5281 atctatacat gctcaaacga ttctgcgacg ctcaccagtg ccgacgagcc gatagatttc
     5341 attgactaca ccatcaccat gcggcgtgat ccaaagacac acggcaagat gatagagtta
     5401 agcaattcgc ttacgttctt tggtgatgcc tacgccttca taaaggataa gtatgacaca
     5461 ttcggcgtcg aggctgacat cgttctttcc atatcttttg actgcgacgg cagcagcgac
     5521 agcctgcctg attcacgtat cgacatgacg tcgctcatgt tcgaggaggg cggaagttgc
     5581 acggtaacgg ctgatctgga agaagctacc tgcatcatga agttcaagaa ccgacaggat
     5641 ctgaaagttg acatcctttc gctttctact attgacgatc ccgacgggga gacgctgacg
     5701 ccgtatgatg gcatcggcat aagcactacg ataccatcta aggctataca aaaggtggat
     5761 gaactgtttt acggtagcga cgattcagta gagatggcga cgacaaccta tcaagatgac
     5821 ttcacggaag atgccgaagg atacatagag acggaaggct atttatggat acgtcagggt
     5881 gatgaacatg gcgagatagg cgggctgaac agcgacttta caactaacag aaattccgga
     5941 gctacggcgc tcgatatatg gactaataca tacgttcaat acgccgatcc taatatctac
     6001 tttgccgggt tgtatagtgt cgatcttgag atagcggctg tacttgacaa tgccactctt
     6061 gacggcggcg gcgcaggtga taagttcgat cgtgttcagg ttgatcttat cgcaaggcag
     6121 accgttcctg gcccttcatc atcggaaacg atattccagt catgggactt caattcaggc
     6181 ccttacgata ataccttttc tgaaaatttg gtttgtgatc tgacggcggc atggcagata
     6241 gcctttcttg aatcatcgtt gccggatgcc gacacggagg gcaactccta ttcattctat
     6301 ttccatgtaa cggtatcagg cacgtactct aagattaacc cgatggcaaa catgggattc
     6361 agcttcagca ctacaataac ggaccgttct gaatcgtatg tgcggtggta tgttatgacg
     6421 cgctttcctg aaagtatttc caagacgtcg aggatcaatg aatcaatatc aagggtggca
     6481 gaaatcataa ccggcgactg ccttcgtatg tattcgtctt gtttcacgcg aaacgacgcg
     6541 cagccggagc cggcagagct gccggaggat gatcctgatt atgtttttcc taccaatgga
     6601 tgtaatagca catacgttat catgaacggc cttatgcttc gtcatatacc ggatgcaaag
     6661 tgctttgtgt cgttccggga gctttacgat tcgcttaatg cgatattctg tttgggcatg
     6721 ggcatagagg atgatcctta ccgcccggga taccaaagga taaggataga ggaggctgat
     6781 ttcttttatg aagatacgcc tatgagctac ggcatcaact atccggacaa gataaaaaga
     6841 agcataaaaa gcgatcagtt ttataccgat ctgacgatcg gataccagaa atgggaggca
     6901 gaggcgttca gctcgcttga cgaatttata acccggcgac agtacactac gtcgctcacg
     6961 cagatcggcg gcaacaagtc gcttttaagt tccatcatag catcaccgta tgccattgag
     7021 ataacgaggc agcagaagtt aacgacgaaa gattacaagt acgacaatga ccttttcatc
     7081 attgatccta actcggacga ttcgttggag ggcggcagtg atgtgataga ctacgtttcc
     7141 tattacaacg ttcttttgtc gccgctacgc aacgcgacaa ggtggttcaa gtggcttttt
     7201 tctatattca agaatcattt ggatgcggat agcaagttga tattctgttc aggagaaggt
     7261 aactacgatg cggtattggt atatcctttc aacacctgcg atcccgttga cggcgttgtg
     7321 gccgttgaga cgacggagga catggatctt acatacccta tcaagtttga ttatgagaac
     7381 aacccaccaa aggtaggctt cgagcaatgg gacttcactt atccgatgag cttcgagaac
     7441 ttccaggagg ttaggaataa tttttatcaa cagctcccag ttcaatggga tgacagcgga
     7501 gtgaagtatg gatatgtgat ggagatggca tataagccgg cgcaggggct tgctacattt
     7561 aaagttttac gcgcatggga agattcagcg acgcattatt tacttttgga ggacggcacg
     7621 tatatattgc aggaagacgg aggatacata atttgggaac cataaaagaa aaagatcatg
     7681 gcaaataaaa aaatatctga actaacagcc ttaactacgc cggatgatgc tgatgtgttc
     7741 gttcttgttg atacttcggc tacggagacg aagaagatga catgggctaa tattgtcgca
     7801 gcgattgagt ctggattaaa tgccgcttca gagacacagt ccggtattgc agagcttgcc
     7861 acaactacag aaacaaggga tggaggtgat gattcaagga tagtaacgcc tgcaaaatta
     7921 ttggattggt ggtcgaatta tatacttcag aatggagttg aattctcgtc atatattaca
     7981 gcgatagagg ctatctctac cgaagatgat ttatatctaa agtcggtagg gtcagtctat
     8041 ctaagactaa caggaacgcc tacggcttat cgtgtcatca ctttgcctga cgctaccgat
     8101 acactcgtag gaaaggcgac aacagacacg ctcactaaca agaagctatc tgattcaacg
     8161 gttgtctttg ggaatgtgtc agatattacc aaagccctcc aattttcttt aggaggcgca
     8221 acaaccggaa aaaagttgac ggttgtttct tcgcacacag ataacaggac tgcaacattc
     8281 ccggacagga ctgggaatat cacaatctgt tctcctacaa aggacgccga tatttcagga
     8341 gtttctacca tcacatttac cggaacaaca ccgccatcaa ctcttaccgg ccgctattca
     8401 tgggattata taagtaacag cataatccaa tgcaatgtga ggcttgaatg gaatagcgca
     8461 ggcgtaggat tgactgctat tgagtttacg cttccgtcgg atatgccaac gccttcattt
     8521 ttcaccggat ggggcaatag cgaattcggg ccgccggcaa atgctgaatt ctatacatcg
     8581 gtaacaggta cgccggttgc gacaagggga tatacagcga aaagcgccgg aggagcattg
     8641 gtaattggtg gaacttgttc atcgaatgct cttataggtg catctttcaa ctttcaatat
     8701 ttcatataga tgatctacgc tcccgaaaac tcaatcatac aattcccggc aagtgatgtt
     8761 atttcatctt gcgtgatcgt tccgtccaag tgcctaccga taacacggag cagcgacatc
     8821 aactttcaga tcatcatcaa cgacgtggag gcggcgccat catcgaatga gtacgttatg
     8881 aagttattca ccacttgcga cacttccgac cttgttgaag tgcctgcata tatatacgga
     8941 actgatgaag aagtgttcgc caccattgca tggacgcaca tgaccgatga aggaatagct
     9001 tatggattta tgacacagac caatgatgac tttgacttcg ggcaagtgtt tgaaaatggt
     9061 gactgtttcc gtatcgggat agtccgggtg aacagcgatt cgtataagta cgcaaatttc
     9121 ttcgaggtca cgggctccga cgtcaccata aagctgacta taaacggagt tgaacagacg
     9181 ctcggcatct tcgactactc aacacctgat gacattgcag atgccatacg ttcatacatc
     9241 accgaccagg ttacggtgtt gcaggccgtc aataccggct atgtgtcaat cactattcat
     9301 tcactttcag gcgacaccta cggaaatttt aaacttggcg gcacgacata cacggcagac
     9361 gtaacggctg gagaggatga tattgttgct tgttcaaact gcttcgtcta taaagccgat
     9421 ccttgcttca ctactgtgct gaagtaccgt aacagcgaga actcctttgg attcaattac
     9481 gaagtggacg aagaatttta caacaaggtg cgggttggaa tgtatattga caacccgcag
     9541 ccagtggatt ctgaaaatgt gtacaggcaa tccaacggcg tgtataagac tttatcagca
     9601 tactttgaga gcgtatcaga gggccacgtc gagtactatg accggcaaag acacttcgcc
     9661 atcgctatgg ccatgcggca tgatgacttt tccgttgcac aaggcaatga aacagagttc
     9721 acgtcatatg tgaggagcgg cgcctatgag ataggatggc tgaacaagcc cggaacgaat
     9781 atcgacagcg ctcctgcatc gttcaaggtg aaagaaacgc catttaatta tatcaactca
     9841 aattgtgtat gacaactgac aaaggcatat tgattatcgc gtgcgggcat cctttctacg
     9901 gacgtatggc gctgaacctg gcggcatcca tcaagtcggt agagccggga atgaatatcg
     9961 cattacgtca tgccgggagc gctgtaaaat acatagatca gtcgcagatg aacgtgtttg
    10021 atgacgtggc gctgattgat gaaagcatct acctgacgga caaaagtaaa aacaactgga
    10081 tccgcacgaa gttattttta tatgacttgt ctccatttga caagacgata tttatagacg
    10141 ccgatacgct atggctgccg aagcggaagc cgtccgatct gttcgatgac ctgaaagaca
    10201 taacgctcac ctttgccaat tacaaacggc atgacttcaa tacggtggat gacaaggagc
    10261 gcgtatgggc taacataaag gaggtgaaag atgtttacaa gttggattca ggttactact
    10321 actgcctgca atcggagatg atctatttca cccgcactga tgacaacagg acatttttcg
    10381 agatggcgaa gatcgtgtat gataatccga aggtgaaaac cacgatcttt gccgggtttg
    10441 tcccagatga actcgccttt tccattacga tggcgaagat gggattacat ccgcatgtaa
    10501 caccttacta ccctacctac tggcacccgg tacaaaagca cgtgaaccgg gataatctgt
    10561 ttgacaggca ttatcttctt agcgttggcg gcgccttcca gaccgattac gtgaaagatt
    10621 tatacaacac acttgcgtca gtagctttca ggaagctaca gatggaaaca ccctggaagt
    10681 actttaacaa acggcaatgg ctgccggaaa gaaaaactat ttaaaatgcc cacaatactc
    10741 acacctgaac ttttaaaagg ttatttcaag gacggcaagc gtggcgccat atacgagcag
    10801 tgtatcagcc ttgagaaagg gctacggata catgtcgacg gcatcatgcc ggaggatatc
    10861 ataggcaggc agcgtccgtc ggagccgaag atcgtcatgg attatcgtaa gcagatatgg
    10921 caagccataa cgaaagaaac ggtgagccgt gtcatcacat gcctatcgaa gatcaggcgc
    10981 tcgcaggact ggtccgtgcg atatgacgaa aaaacatttt cggccaagat cgcaaagaac
    11041 gaaacaccaa aggaatactt tgaaaagaag ttcccgtatt tcagctcgct tacaaactgg
    11101 cttttttccg tttgccttaa ggaatatctg atagatgcca acgctgtttg cgttgtgatg
    11161 cctttggatt tcacgccaat ggatcctact gaatatctac agccctaccc ttacatcttt
    11221 aactccggac aagtgtatga ctatgtggtg aacgattatg ctgtaataag gtcgaaggac
    11281 acggcgacat acatgaccga tgacggcaaa gcaaagcgca ctgatggcgc catcttttgg
    11341 gttataacca ctactcatat tttgcgatat gagcaggtaa atgagaaagg ggatcttgca
    11401 ataaagatca actatgagca ttcgctgggt tacatgccgg cgttcaaact cggcggcatg
    11461 tacctgaaag ccgttgacaa agtgccggta tgggaaagcc gtataaattc aatcgtgccg
    11521 cgactgaaca aggcggcgcg tgaggataac gacttagata tatccgtagt ccgtaacctg
    11581 ttcttagaaa aatgggaata tgtttcgcag gaatgcaaca agtgtaacgg acttggtaag
    11641 gttaacggat ccagtggatt caccacttgc gaagcgtgta acggacaggg caagatcgtt
    11701 tcatccccta tgcaggttca tgccatcaag ccggcatcac ttaatgaaca gccattgccg
    11761 acgcctccgg caggatatat cgagaagccg gtagagataa ttcggatcgc agaggaaagg
    11821 atagaaaagc atctatataa agcgcttgcc gctatcaata tggaattttt ggctaagacg
    11881 ccaatgaatg aatcaggcct tgcaaagcag gtggataagg atgagcttaa taacttcgtc
    11941 tattccattg ccgaggatat tgtattcatt attgacaatg tttataaggc gggatatgac
    12001 tatcgttata aggtggtggt tcctattgac agcgagcgat acgccatgct tccggtggtg
    12061 aacgtgccgg agaagtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
    12181 gcgggaagta atggagaaga tggctgatga aaagattgcc gaaatggacg aagcggcaaa
    12241 gttgaaagct aaaatgtttg cttttgccgg tgtggcaaat ccgaacggcg atccgaataa
    12301 gaaagaaggc gctgctgcat aatggatgat aagatcatag agcagttaat caccaccatt
    12361 gacgattcga taacatcgtt cactggcgct attcctgaca ttcaacgaca gatatttgac
    12421 gacatcaacc tgcttgtcaa agaccttgac atcacttcgg atgggacgat aaaaaacaat
    12481 gttgcaaacc tgaagatcat cgggcgtatt cggggcaagt tggaggagat cattctttct
    12541 cctgaataca tgaagaaggt ggaagacttt gtaaagacgt tcaatgaagt tgccgacctt
    12601 caaaataagt atttccgttc tattgaagga aagttcaagc cgacaaagtt gctggatgaa
    12661 ataagcaaac aggcaattgg atggacggtt gacgggttga cggcttcggg gattggagcg
    12721 aatgtcacgg atgaaattga aggtatttta aggacaaaca ttaccactgg gggatcatac
    12781 aaagacctcg caaatcaatt gcgagattcg atacttagca acaaatcagg tcagggattg
    12841 cttgaaaggt atgtgaggca ggtaaccact gattctatca tgcagtacaa ccggcagtat
    12901 tcgcactcca tcagcgagga catcggattg caatggtata tgtacactgg ttccaatatc
    12961 aacaccaccc gcgctttctg tattgcttgt actgagaaga agtacttcca taaatcagag
    13021 ttaaagccgc ttgtcaatgg tgacttattt agtcggaaag acgttccagt caatcctaaa
    13081 acggggcttt ggtatggcgc aattgagggg acgaatgcaa gtaatgtgtt aatcaatgcg
    13141 ggcggctatc aatgtggcca ccaatttcag ccggtccttt catccgctgt ccccgaatcg
    13201 gtgaaggcga agtttcagta agattgctta tatttaaccc caataaaggt gcaacccata
    13261 gcgcaattgt tttttcttaa tgggtcgcaa aaataccaaa tttaaaaaac gaaaacaatt
    13321 tttgtgaccc atgctgaagg gaaaaataaa cacccgtaaa agattaacag accgtttatt
    13381 tagctttgtt gcctataatg tatatgtctt atgtctaatt tgtatgggag gaaggaaaag
    13441 aaaaaaaact ttttcttcat ttcaagaacc taaaaatatt actgaacatg cttattacat
    13501 gtccattgca gcattaatat cagtaacggt atcaggattg ctacaagtat tgatattttt
    13561 gaatatcgta acgagtcaga tgcttgtttc ttggtttcag gatagtcttc aaattctctt
    13621 ttaagcaggt taacttcata ccataatttg tccatttgaa gggattctga ttttttgatt
    13681 tctttcaaat aattcaggta ggagccatat ctttttaagc cattaaaacc taatgggctg
    13741 atgtagtatt tgtttggttc atttccaaat ccttgaaaca aatcaaccca tccagtaata
    13801 gacaatagat catgcagatt tcttttaatc ggttgcttta atttatattt aacataatcg
    13861 tcaagagaat agaaattctg accattaagg ctcatatatt ctataaagtc atttagcact
    13921 tcatcgtcag acagtctttt catttttctt ttcccttctg accttcaagt taacataaga
    13981 gcttattata ggctttcgat gcggttccct tcagcatgga attaagaaaa aacaattgcg
    14041 ctatgggttg cggcgctgct attgtccttc ggactgtttt tgtttaagta atatttcagt
    14101 ttactaattc aacaatttgt ttatttttgt tgtatgccaa tagctaccat aaactttgaa
    14161 ggcgaagtat atccattttt tcaaagtcag ggtttcgcaa gtcagtacgc ctttccattc
    14221 gcaaaacaag tatgtatagg aacaggattc gatataggtt gcggaaaaaa agaatggtca
    14281 ctacctgatt cgattcctat tgacaagaac ctccctgatg aatgggacgc aatgaacctc
    14341 ccgcctttta tggtggactt catattttcg agccactgcg ccgaacatat attagatttt
    14401 gtaaaaacat tcgattattg gacttcacgg ataaaaagcg gaggtgtttt gtttctttac
    14461 ttgcctcact actcacaacg atattggagg ccatggaata acaaaaaaca ttacaacatt
    14521 ttcactcctg aaatacttcg ggactattta caacaagcag gatataacac aaataaaata
    14581 ttcgtttcag aagttgatct gtacaattct tttatggtaa tggcagagaa aatctaagct
    14641 atgaaccaaa tctggaaaga gatttcgggg tacgaaggcc tttatgaagt ttcaaatgat
    14701 ggaagggtaa ggagtttaga tagatgggta acgcatcctt acggaatggc attaaaaaga
    14761 ggaaggattt tggctgctgc aactatatgg tctggatata aaagagtgtt gcttattaaa
    14821 gatagcataa gaaagaatat aaccgttcac aggcttgttg cgcttgcatt cgtggaaaat
    14881 ccgaataacg aaacgcaagt aaatcatatt gatggaaata aggagaacaa ctcttttttg
    14941 aatcttgaat ggtgcagtat aggatataat aataggcacg caagaaatac cggattaaat
    15001 gttgcaagat ttggtaaaga aagccacagt tatggattta aaaataaacg atctcacgcc
    15061 cttagaaata ttgaaaccaa tgaaataaaa cccattgttg aagttgcgaa agaatatccc
    15121 caatggagtc gcaggtacat tacaatgatg ataatggggg ggagagggaa caaaacgaaa
    15181 tatgtgctac atatatgaca attgcctttt gcacatacgt ttccgacaaa tattaccact
    15241 cctttggcgc ggataaatta cttgcctccg ctaaatattt ccatcccgac attccatttt
    15301 ttgttttcgg ggataaagag atagcgaagt ttccgctccc tgttgaatcg ctgcacccct
    15361 tcatgatgaa taagctgatg gcaaagtttg acatcgtagt tcacattgac gctgacagta
    15421 ttatttgcgg ggatattaca cctgtatttg aagcacttga aaccgccgat gttgtttgcg
    15481 tccgaaacaa taacgactac ggaaaagcgg gatgtgataa ggcattgagc cagcaaaaca
    15541 gggatataaa tatctatgtg aatgccggat ttgtggcaac gaaatcaaaa gagttcgtgg
    15601 tagattggat gcacaataac ttgctgttcg ctgaactaac gccctttggt tcacagtcag
    15661 tcctaaacac cataattgat aaataccatt ggaagctgat agacggcatt gatgacgatt
    15721 gttattacgg cgtgtcctgc cttcatggtg aaaattcgca ttgggaaagc tggcaaagga
    15781 ttcaggtgga tgaaaacggg aatctttatc tggaaaacta tgaacgtgct aagactgtga
    15841 aggtgctgca ccatgccgga gggttcagtc cggataagtt aggcctatat cttttcaacg
    15901 atgaaacgcg gaacaggtta actgaaatct gctatggtaa aaaaatcgga gattgagaaa
    15961 agaatatcat ttctcaacag caaaaaaaag tgtacaaaaa agagttaccc ggacaggagc
    16021 gttgcgaagt atcacctaaa gaatgtgaaa cttttgcatc gcaatgaaca gaatttctat
    16081 tactgccacg actgttcatc atggcatctt acatcatggt cgaagaacaa gagcaggacg
    16141 gtttttaata acaaaaaata aacatcatga aattaggaat cgtgatccct gtttataaca
    16201 ggcctgaata tttacggcag tgtcttgaca gcgtaatacg atcaatattg ccggaaggaa
    16261 ttaacatcgt cgttgtgaac gattgcagca cgcatcctga catgaaccgg atactggcgg
    16321 gctttgctga aaacttgcgc ggcaaatcaa taacattcaa aaataacagg gttaatagcg
    16381 gcgttgcata ttcgatcaag gccggaatag ctacgcttct tgaggcggga tgcgatatgt
    16441 ttatcaatct ggattcggac gctattgtaa ggcgcgaatg gattgataaa ataatagagt
    16501 taaaaaatct tttcccttcc aagatcgtaa ccggtttcaa ctgccttaca aaaaatgaca
    16561 atggtactga acggcatcct ataacagagc aagcgcaagg atacaatttc aagaaatcag
    16621 taggcggtct taacatgtgt tttgacagga cgcaatataa ttcgtgcata cttccggctc
    16681 ttgaaaaagc gatacggcgt gaatgcaact gggatgccga ggcgtgtaag tattcaggtg
    16741 gcgcggcgtg tgtagtgccg tccgttgttc agcatatagg gatcaattca agcatggggc
    16801 atcacgaaac gcccgacact gccgatgact tcgtaaaaga tgaaatttta cctgaagtag
    16861 caaaaaacgg cttcattaca ggcgatataa gcgaagaaaa aagacagggc aatacaaagc
    16921 tatcattgcc atccgttacg cttgttaccg ttaactgcga tgcagtagaa gcgggaattt
    16981 ttgccatgaa taaaagcatg gagggcattg acttcggatc cgttaaattt ctgacctcgc
    17041 aagacacgga ttatgaatat gccgttaaga ttccacacat cgcaagcata aaggagtatt
    17101 cccggttcat ggtgtatgaa ttgtataaat atattgacac ggagtttatt cttgtaatcc
    17161 agtcggacgg atatgttatg aatccggcgg catggacgga tgatttcttg aagtatgatt
    17221 atatcggagc gacatggtgg ttccgtgatc ggcataacgt gggcaatggt ggtttttcgt
    17281 tgcgatccaa aaagctgatg gagattgtcg caacggataa gcatatacga atttatcacc
    17341 cggaggacga ggcgatatgc cgtaagtatg ggagattctt gcaggtgaag cacggcattg
    17401 agttcgctcc tgataatgtg gcaaagaagt tcagtatcga agggtggagg caaaacgaca
    17461 agacttatat aggtcagttc ggatttcatg gacgtggcgt aatatttcca ggcacattat
    17521 ccgggagcgg tacaacgcaa ataaaacagc cggaaagaaa gccggatatt attgtcatca
    17581 atcagccgtt tggattgggc gatttccttt tctgcatccc actgatgcgg gactatatta
    17641 acgaaggtct taaggttatt gtgccggtga tcgggcagta tgataatata aataaacact
    17701 tccacgatat tcttttcatc aataaagaaa tgctaaagat caactacgag cggaaggacg
    17761 agtacactat ttcaggaatg aaggtcatcc cgctgcggtg gagttatgag attttaaaag
    17821 tacctttttc tgactgcatg aagtcgaagt acatgatgtt tggcaaggac tggacgaggt
    17881 ggcgggaatg tacatggatg cgggacatgg aggcagaaga tgaattgtat tacaaggtac
    17941 tacaattgaa ggatggcgag caatataatc ttatcaatac cacgttccgc aatgacttat
    18001 ccggtgaggc aaaggttaat gtgaacaatg gatttaagaa tgttaagatg cggacggtgc
    18061 caggattcac attgattgac tggggaaagg tgattgaaaa tgcaaccacg attcacactg
    18121 tttcaacttc cataaattac atgatagagt tgatggcatt gaaggcgaaa gaaattcatt
    18181 tgtatgtgcg aaagccggat gaaaaagatt tccgtaatat caactacctg tttacgaaac
    18241 actatcatct tcatctttga tttccgattg ttctaatagc ttttttactt ctttggtgag
    18301 ttccgattca attgattcag gtagattttc ttcgccatct tccatgccgt caaatattcc
    18361 atccattgta ataactgttt tcattgtggc aaagatattt taattccctc gaaatcgagg
    18421 gaattagatt ttatcaatta ttattatatc taataatcac cactatcaga acagctattt
    18481 ttaccaaatt caaaaaacca tttgcctttg aaaatgaaaa taatcagatc agcaaagcaa
    18541 agaaggaaca gcattccaga aacaacaccc aatgcaagca aaaatatctt tgataaaaga
    18601 ttgtcaaaca ggataaaata tagcattcca ataaaatctt ttggtgtgcc atcttcataa
    18661 taatggtctt gcttaaagta atttgtgtaa tctgaaaagt ttttcatgta tagttttttt
    18721 tgaatttaca tctcctccac catcttccac accggaacgc cgtttataac atagttgcgg
    18781 atttttcccc agttcctgat gatcttaagt attgttttca cttgcgaaaa cttgcatctg
    18841 cattttatct gatgatcggc ctgttccttc agtataaccg acctgacatc atgcgggact
    18901 tcattgataa gaacggagta ctttggcata aattgtaata atttataaca acaaagctaa
    18961 taaaaaatat cattaaacac gactggatat tatttttggc aaaacaattt ccttttctcc
    19021 atgaaagtaa gcgagttcat tcagaagttg gccgcaaagt caggtatcaa tcttgccgat
    19081 gaaaaaaaca aagacatcgc tacttcgata gcttctatca cgactgaact tcctgatgaa
    19141 tccgtttcta agatgcttgg aagtcttatg agcgtcgatg acgcgaaaaa taattccgac
    19201 ctaaaaaagt acttcggcgc gcagattttc gacggcatgg attctgaatt ttcttccatc
    19261 atggaagaaa tgggattcca ggtatctgac cgggaagcga tcaaagccac cgattccgct
    19321 ttcaagaaga taggcgccat cggtcgcaag atcaaagaac tggaagaaaa gaaatcagaa
    19381 agcaagggta aggacaagtc ggaactacag gaaaagatca atacactgac ctcgcaactt
    19441 tcgcagctta ccaaagatca cgacaataag atcaaagaac tgcaatcaac ttacgaaaag
    19501 aagctaaccg attcgcaggt acgttccatc ctgaaaggga agaagtacgc gaatgaccag
    19561 gtaccgcaag atgttaatgt tgagttagcc cttgtccttt tgaacaaggc gctcaatgaa
    19621 aataaggccg tttatgttaa tcaaaacgga tctcttgtcc tgaagcaatc gcaggacaat
    19681 accctcgatt ggatgaactc atccaatgaa aaaccctctt tcgagcagta cattgacggc
    19741 gtcctcgccg ccagtaagtt actcgctgtt actccaactg ccgctgccgg taattccgga
    19801 gcgggaaata atggcgccgc tactatcatt acaggaggcg cacaaaacgg agctaatgca
    19861 agtgttatga acgcctttga ccagacgatc agagagcttg aaagcgctcc gctgatttaa
    19921 ccactacttt caaaacacct ttttactaaa atggctacag gcttcgcacc tgcgctgctt
    19981 aaatctatga aggatttagc aggcataaac tatcccggca ccaaaataac tgctcccgga
    20041 tttcttaaaa tgttgctcca aaattccgac atcatgggag caaaactcac aaatgaacag
    20101 ggccatcaaa agacggctac tgtaaagtac aaacagcgta tctctccgtc aatggtagct
    20161 accaatgaca actgcgacgt ggacttttca ccggctttcc gtgaggcatc gctgactgcc
    20221 gtaaacacgg ccaaatgggg cgcccatatc tccctcgaca ctgtttcccg ctacatggaa
    20281 gaagcaagcc gtggcgttac cattggtggc gtatccgtca ttcaggaagt ggccgacacg
    20341 gttacacagg ttgccaatgc gctcatcggc aaggttgacc gcgtgttgct tgacgctgtt
    20401 gactggggaa aaaatgttgt ttacggcgat gacttcgttc atgacctcaa cttcgaatcc
    20461 gatgctacct tgttcgacgt aggaaccggt ttcccgaagt tgctcaatga tgcctttgaa
    20521 aacgaattct ccggcgatct gctgatctgc ggtaacggta tctttaacgc cgttgagctt
    20581 tctaagatgg cttcgcaggc tgcattgaac ggcattgaca cctcaaggtt caccggatat
    20641 aagtggtacg ctgacctttg gagcaagaac tccactaact ggggtggcaa cgacatcggc
    20701 gtattttcaa aaggatccat tcacttcgtg gacttccaga agtatgtagg attccgggcc
    20761 ggcaagctcg gtacgtcggt attctttcag atacccatcg acgtggatgg cgttacaatg
    20821 ttgttcgacg cgcagctgcg tgagttggac tgcccgactt ctattcctaa tatttacggt
    20881 acatcaacgt cgtatgatcg cggatacgtt ctttacctga ctaagaccta cggactgttc
    20941 caggttccgt ccgacgcctt cgcaacggat gaccgtctga caggagtgaa cggagcgatc
    21001 cactacaacg taacaaactc ctaatttttt caccgccgga tgggttatca aaagcccatc
    21061 cggtttttaa aaatcgcagg gtagatcagt tggccagatc agcagattca taacctgcca
    21121 gtcggtggtt cgaatccacc ccctgcaact attcacctat catggaatgt ttacgcgact
    21181 ttgtaggaat tgaaggatgc gacgcgccgg agccgttgtc agggttcacg gtcaatgacc
    21241 tgcccggcat cacgtcgttc atggtaggta agatagcgag caatgaacag gttacctact
    21301 taggcgcatg gaatgacatt caggagcggg cgttgaagaa attcacgtcg gccatcatct
    21361 ccgctttcca taatgacaag aagcagcgta ttaagagcgt aacgcaaacg gtgaacttag
    21421 gacggcgtat tgacgcggca agcgttatgg cggcatcatc acaatatcgc ggcgtaacaa
    21481 ttgaactggc cgcaaatggc cagtccgcaa tgctgcaaag ctcgctacag gtaatctaca
    21541 ttcagcaggt atctatttac gcaacgcaag tatattctga ctttgagatt aaggtttacg
    21601 atctcgacag cgatacggtg atagacacta tcacttcaga cctcgtcgaa ggatggaaca
    21661 tcgtcagggt agctacggcg tatgatgcca cacggatatt tatctgctac gatgccgctg
    21721 acttcgattc cgtagaattg cttattgacc gtaccgttgc aagtgattgc cagatgtgcg
    21781 ccgatgccgt ttacggatac ggtaactgca aagcggctat ctatggcgcc gcttcggcat
    21841 cactgtccga catgacgata gacaaagccg ataacactta cggactttcg ccggtattct
    21901 caatacaatg ccgctatgac tgggtggtat gtaacaatat tgacctgttc accctcgcat
    21961 ggcagtattg ccttggcgca gaggtgatga cggaacggat atactccgac aggcagaatc
    22021 gttatactgg cattgatctt gaaaaggcga aggcgttaag ggacgaattt caaaagggct
    22081 ttgaggtaga attgaaaaat gccatatcag gcattgacat agacgaaagc gattgttgca
    22141 ttgaatgtgt ttctgttatt cagaaaaact actccatccc atgatcgccg ttaagataga
    22201 cacgcaaaac ttcgcttcgc cggataagtt aagcaggttc atatctgccg acggtcaggc
    22261 tgattttctt cgcgccgttt gtctttctat gctgccggta gttcatgacc gcatccatgt
    22321 taacggcaag gctgccgacg gatcaccaat aggcacctat tcagatttgt atctgaaata
    22381 cacgcgcccg aaaaacaatc gtggaacgga tccaaaagtg attctatcac ttacccgtca
    22441 gatggagaat gatttctccg tcgtggctga cggatcacgc tacggacttg ggtttaagaa
    22501 cgaggtgaac gcgcaaaaaa gcgaatgggc tgaacagacg tataaaaaga aaatctattc
    22561 gctgacaaat gaagaaaagg aacaggctgt aaatgccgca aatgaatacg tgaatggcgt
    22621 actataaaga gatcgttgat gtgattgatg atgccatcgt agccggtgcg cttaccgatt
    22681 cacgttttca gaatcgcgcc tttgccgggc tttgcttcct tgtaccttgc aagatcaacg
    22741 acacgaatgt gtcggcaatg ataccggcta aggtttcaac agtgggcgaa tgtaacttca
    22801 tagttcctga cgataccttc aatctggtaa catatcaccg tgtgctttcc accatttaca
    22861 ggccttctat ttcacaatac ggcgatggca cggattacat agatgctacg gctgatatgg
    22921 tgcttacggt aatatcattt accgataaac ttcgtattca gccggaaaac gtggaggcgc
    22981 ttttcatctc cggtttccct acgtcgtttt catctgcctt aaaggaatct ttacagctaa
    23041 gatcactgaa cgcaatcata acggcgtcct cattcgatca ggtggcaatc ttcaacagtg
    23101 agtaccgtgg cgagacgtat ttcctgaagc ctggaacagt gatttttcag atcagatacc
    23161 agataattca atcattcaaa aaagactgct tcacgatctg ttgtgaagcg gaagcataat
    23221 ttttttcaaa caccataaaa acaaacttca ggcaatggct atttattatc aggacacatg
    23281 cacggacgaa tcagtagagc atttttgcga tgactgcgag gcgaaagaaa gcgcccgaat
    23341 acgttccgtt tttttcgtta aggagggata cgaattttcc gatcctaccg acaaaaccga
    23401 atggcagacg gctgccgctg ccggcaatgt ggtggtggtt ccaaagacac gcggacagtt
    23461 cgacggcgga tcacccaact acggaccggg cttcggcgac gaagaacagc gttacatcaa
    23521 ctccacgttc aagctgaact acttcgatcc caacttaaag aacaactacg agttctacga
    23581 ggcgaaaaaa cgtagcaaaa catggcgcgt tggataccgt accgaatcgc ttgtttggat
    23641 ttctgacaaa ccatgtacca tagcgccgaa ggctgccgta tcggacaata tcaatgaagg
    23701 cgtggaatgg gaggtagagg taacatggaa cagcgcgaat aacccggaac cgtttaacat
    23761 gccggatgat attttcacct gcttcctcgt atcctaatta tggccagtaa gcaatcattc
    23821 atacgagctg ctttgagttc ggcaggtgag ccttcatcca aaagggtact ggcttgcctt
    23881 gccttcctcg tttcagtcct cttgatatgc ctacagatca tttttcctac aaatgaaaca
    23941 ttcttcacgc attgcttcga ttcactgctt atgttttctg ccgcatcgct cggcggtacg
    24001 gtaatcgagg cttttaaaaa acaagatacg ctatctgtat gagtgagata accataacca
    24061 catcagacac gccgttacgt gatttcttcg cttctaacag cagtacaaga atgttctttg
    24121 agaagcgcgg taatgacatc gtatgtaagg atccgcttac tcacgaatgg tttgacaggt
    24181 atgtgaatga aggtggtttc ctttgtatct cacatggcaa cggtgctttc agcaattact
    24241 acggcattga tccccgcccg cagtatttag gcggcggaat gggatgggac gccgtgttag
    24301 ctcctccggt gaactgttct atcttgaaag gcgatctatg cacgccatac acacttaacg
    24361 ggcagacgtt tgacttcttc tgggcgcata acgcattgcg taacaggatg ggagcatcct
    24421 cttacattat tgcgaatatc ctacagggaa caatcgagca agtttatctg cagattgacc
    24481 aattggatca gaatatcata tacattcaat tcggcgaaga aacgacggac ttatggacgt
    24541 caggcgttga gtatgcgaat aagtgctacc aatgggcaga cgccttacgt gaacattatc
    24601 ctgggcaacg cttttacttc atgtttgact gcccgatgtt atggaagaag gacgtaggga
    24661 caagcgccaa agcgatgata tgggcgaatg agatagcctc tgtacgtcct gttgacaagt
    24721 cattatttgg aatacggcag tactgccatc tgatgtattt cattaatgca aatgatgaca
    24781 cggatcataa catcgcggag gtggagaacg caataaccaa cctgctgcca caatacgccg
    24841 ccgctattga atcatcacca tttgccgggt gcaaagtatt cataggacag atcagcgcca
    24901 acgagggcgc atacacgccg gaggtgaata agggcgtaca atacttcgtg aatgcctacg
    24961 tgcggatgat gaagttcttt accgataact tacgcgatga tcttacccgg ttcatcgggg
    25021 tatgttacat cggaacaaat tcatggattg acaagtcgct taatacaaac ctggatttcc
    25081 agtacgtcgg cattctgaat cagatttttg ggggggggca aaaagcgctt tacatcaacc
    25141 agttcgatcc gatggttgac gttatcggaa cgatgaaaaa cggcgtttat aaaatcgtta
    25201 tgcaaaacag gaaaggggag ccggtgaagc tgccggaaac aattaccctt gatggcattg
    25261 cagtaccttt gtcttcagca aatgtacatg cccgcaaatg cgcggcaagg gattctacta
    25321 cgtcaacaga ttacaatcct ttgcaggatc tgacattaag cggatattca attcaatgtt
    25381 ttcaaattaa accataaaca ccatgacaac gaagatacta acccgtgagg agtccgcgct
    25441 tgtagaggcc ggacgcaggc agacaatatc gccgcttggc gatctatggg tggcgctgaa
    25501 atgctacggc aatgacgacg ccttcaatga aatggccgtt caatacaaca gcattgaaga
    25561 taacgagatt gacgccgtca tagatgccgg aggaacggta atggaaacat acggcaatgg
    25621 tgatgtgaag cagatcagca aggcgcccaa caggcctaag cctccggtaa cttaaaaaaa
    25681 taagctatgc cgaatagtag aataaggttc gatatggttc acttccgcaa cctgcttatc
    25741 atattcgtaa cgattatggc cgtgatgtgg acgtatatgg caatggctga cggaacagcg
    25801 acagcagacc gttacatcac cgtagaccag gacatatggt cattcttaaa gtacgtaggc
    25861 gttatgtttc ttggtattct ttcatgggct gccgttcagc tcatatcatc actgaaatca
    25921 cttgtgtcgc aggttaccga tcttaatatc acggtgaagg ttacgcaaaa tgagatcatg
    25981 ctgataaagg aggacgtctc cgagatcaac agcaagatag aaaaacagga ttaccggata
    26041 ctgcaattag aaaaggcgca aaagaattga aacagttgct attcatatat ggctacttcg
    26101 gcatcaatgc actggtgatg aacttttacc ggatttatca atgtgttaca tgcgttgagt
    26161 tggattacca gaccgccaac gatatgtgga acgcttcgct tatgattgct atggcgttgt
    26221 catggccggc attctatttt ttgaacaggt ggaagatcct tccgtttgat ttctgggacg
    26281 aggtgataat cttgatctgc tttttctacg ctatgattga cgcgatggac tggttctgga
    26341 atttcaatat gaggtcagac ctgatggact gggttacgtt tatatttatc aacttcgtta
    26401 tcgtaggagt aaagatgtat ttcaacacgc aaaacactaa ggcgcggtag gcctatttta
    26461 tcaccacttc accggtagcc agtgccggct caatatcgca atagtagttc actatattcc
    26521 cgcccgcaca aatcagataa gtctttttgc ccattgtctt gttttcaatt gtctttatgg
    26581 acttgatgtg aaacgtagcg ccggcatatg cggagccaag atttattttg cctgttagtg
    26641 aaacagggtt tgtatagacg aatatgaaat ctttcatggc atctgaacct attccaagat
    26701 ggagctcgtc gccgggcttg aatgtgtgcc cattggaagc gtagtagatc gtggtggatg
    26761 atggattgct ctgcgccagt accgaaaaac acgacacgac aaggatgata agcaggattg
    26821 tttttttcat gcaaacgaag ctacaaaaaa atattttgat aaaaagttgc aaattggaaa
    26881 acggtatgta tatttgtgta cgattaaacg tattcaaaca atgaacagcg aaaataaaaa
    26941 ggttaaggtg cctatttctg ataagttatt tctgcgatgg aagcagctgc atgagcatgg
    27001 cgactttcag aagatggcgc gggaatacaa tatcccggtg cataagtttc agcgcgcttt
    27061 ttcatccagg aaggctgaag tgagcgttgt gaggtctatt aatattttct acaaccggcg
    27121 cgaaaggtta caggatgccg ggctgaaatc actttcaaac attgaaatga cgtaatgctt
    27181 tgcgaaaaac aaaaatacct gcttcgccaa ctggcaaaaa tcaagatcaa aactattctg
    27241 catgacacca agaagaaaag aactcgtagt agaaatcggc gcctggatga tagttttatt
    27301 tgtgatagcc ctcctgttaa aattcattca atcataattg tttcctaacc attaaaacca
    27361 aaatgcaatg acaaaaaaag cagaagaact acaaaacctg atagacccga cgacgccggg
    27421 gcaggtggca gagccgtttg aatttccaat ggcctgcatc tatcttgatg acagatgcct
    27481 tattctttca gaaaagaaat ccgtccgcat cgaaaactac acgcattcca atacggcacc
    27541 cttcgagtat gtaaagagga agtacgcgga ggaactggac gtaatggaat tgctcaatga
    27601 attggagggc atctgccgga gcatcagggc taaaatgttt ctgaaaacaa cttccgaaaa
    27661 ctaaaaaaat ccccgccgga tgacaaatcg cggcggggat aaaccaaaat gcaaggacaa
    27721 aagtacaatt ttcattaact ataaaaacca aaataaaatg attacagcaa ctaacaccgg
    27781 aggcggagat catactccgg tagcagcggg aacctatccc gcaagatgtt tctcaatgat
    27841 tcacatcggc accatcgaag aaaatatcat gggagaaaca aaatggctta acaaggtccg
    27901 catcacatgg gagctgccta cagaaacgaa ggttttcaac gaagaaaaag gagaacagcc
    27961 gtttgtcatt tctaaggagt tcacgctttc gatgaacgaa aaggcaaacc tgcgtaagtt
    28021 cctggaagga tggcgcggaa aggggttcag cgatgacgag gcgaaagcat tcgacatcac
    28081 cgccctactc ggaaagccgt gtatgctttc cgtcatccat aagaccagca aaaaagggaa
    28141 cttgttcgcc gagataagtt catgttccgc tatgccgaag ggattcacct gcccgccgca
    28201 gataaatgac acgcttgaac tgaactatac cgaccactgg agcaatatcg agttcagcaa
    28261 gctgccgcag tttatcaagg ataaaatgac cggatcgaag gagtttaggg caatgatgaa
    28321 cccgcaggaa gtggaagcgc caagcgagac ggaaaatctc tcacatcaaa cggctgatga
    28381 tctgccgttt taaaaaaaac agacacatga caccatcact ggcatcaaca acatgggacg
    28441 actatcatcg tcctcctgct ccaaaacgta tagagccgga aacaccgaag gcaatagatg
    28501 tgatacattt taaggctaca aaagaaaaca ttgagatcac ctattacctt gaggggatac
    28561 tgaaagaaca gtttaaaagc gttgatattg acaccttgcg gcaatgggct attgatgaca
    28621 agaacctact ggaagtgtat gaactgccgt gctacaacag tgataatgac gaggagtaca
    28681 ccgtcatcta tcctgccaca tcggaaaccg gaacggctga acagatcaac caggcaaggc
    28741 ttgacgcctt cataaaaggc cttacagctg aatccattga gatcatgctt gaaaagcatt
    28801 tgcatatctg aaaatggccg ctgacctcac atacttcgca aaagtggatg aaaacggcgt
    28861 cattcaagga ctggcgcgta agaagttcgc cagggaggtg aaggatatgt tctccggcaa
    28921 aagcattgag atcacggtac gccggaagaa aaagcatcgg agcgttcagc agaacaggta
    28981 ttactggctt gtggtaacga tgctatccga ttacaccggg ttcaccaaag acgagatgca
    29041 cgcgatacta agaagtaagt ttttacgtgc tgaaaaggtg aatgaggata ccggagtcgt
    29101 ttatgagtat gtgaaaagca cgacggagct gacgacggtg gaatatgaag agtatttaga
    29161 tgacgtgcgg cggtttgcgg cgcaggagtt caatatgaac gtgcctatgc ctaatgaaca
    29221 aattgaagta ttttagttat ttgttttggt aatcctcccg ccatcgtaaa atcggcggca
    29281 tggagaatgc ggcgggaggt tttctcttat ctattaaaaa aacataccaa tgacagacta
    29341 cataaccgat caggatccag gcgacgagca gccggagcaa gaaatgataa aacttgtcat
    29401 aactgacaat ggcattttcc ctataaaatc tgttagctat gcaaattgaa atagacttcc
    29461 ttcattcaag agaaaacaac gctgaaagcg agattaatct tgcaaagcaa gcggacaaac
    29521 tcaataagca atgctcgcag gtacttaaca tcctgatgac aggcgagaaa ctgacttcat
    29581 ggtcggcaat ggtgaatcat catatcgggc accttgcccg ccggatatta gacctgaagc
    29641 agaaagggta cgctattcag gatagatgga tagaggagaa cggatccaga ttcaaagagt
    29701 atttcatgaa ctaaataaat aaacaaatgg caaagaaaaa gattactttc agcatcaacg
    29761 aatcgggccg tatgatcggc gagagcttca caaacatcga gcatggaaac ggcgaaatca
    29821 ttttcacgtc cgaaaacaag actcgcgtag aggcaaacct taaagccgtc aaaaggctga
    29881 tagtagccat ccaggaagga cactacagga ttgtaaagcg tggccttaat ggtgaggtgg
    29941 aaaaggaata caaataacgt aaccggagcg cggttaaagc ggatgccgaa aagagaaatc
    30001 tgccgggaca ttccgaatat ttcgataatg cctaattaca tcctacaatt agccggctcc
    30061 tttaatcctt aaagaaatga gcgatcagat caaaattgaa atccaactca ctcctgaaga
    30121 aatagaagca tggataagaa acaacaacca aagcccagtt acctaatcgg cattgatcca
    30181 ggtataaaca acggcatcgc ttatttagat accaataccg ggaaactgat agctgtaaca
    30241 tcatgcaacc tgcatacatt attcaatctt gttttgtcag aattagataa acctatattt
    30301 gtttatatag aaaaccctaa tacatgggtg ccgttcaaaa atgtttcaat ggaagtgatg
    30361 gagatgaggc gttcaggcgc cggtgccgtt aaacaaacgt acaggcacat catagagttc
    30421 ctggaagatc atggcattga atatcgacct accaggctac agggtagcat gaagaaggtt
    30481 tcatcggaat ggtttaagaa atttaccgga tgggccggaa agacaaatca gcacggtcgc
    30541 gatgccgcta tgttggttta tggtagaatg tagaggcaca agagtttcag gatagttgcg
    30601 gaatgtataa tgttggtgat acgttgctgg gaggaaaaaa acattaaaca aatttcttga
    30661 aatactcggt gcagatgtgc taccagagaa tagggaattt gtatttatgg agaaagcaaa
    30721 ggagtgtgca attttatgtg tggatgaaat ttggaaagaa atgaaatcac atgatgactt
    30781 gaagtcggta aatgtttttc agtatgcaga agtaaaatca ctaatcaaca aaatatgaca
    30841 cacgcattaa aaacaatcaa tccattttac aaggcagtag ccgatagaga aaaaaccttt
    30901 gaagttcgga aagcagatag accattttcg gttggcgaca aacttctgtt acaggaatac
    30961 agcgaacaag gcggatattc tggacaagaa gaagaattta gaattaccta catacttgat
    31021 ggcggtcagt tcggaattga aaaaggtttt tgcgttcttg gaattaaaga aattgaaaaa
    31081 tattaaaatg agggtgggaa aaagttttaa ttcattactt aaaaatatgc agacagattc
    31141 attaaaagac aaaaccccca ctattggcaa tactttttta tatgctgtta aaaatgggca
    31201 gttgaaaagg tggttttacg agttccgaca cggacaccct tttctactat ccgatgaagc
    31261 agatataatt gtagaaacaa gattgaatga caaagcaaac caatggatga atgacgagca
    31321 gattaaagaa ctcgtaaaac ttagagtaaa agcaatgtat aaaaagaaat ttccaaatgc
    31381 aaaacggtgg tcgttgccca tttttaattg catataacgg ttcggggctt aatgaagtgt
    31441 gcctcgctca accgttcaaa attaccacta cacttcaaag gcacatttca tttaagccct
    31501 tgttatggga tgtgttttta aaaattagca ggggcaaatt caaacaatta gaaaatgaaa
    31561 ctcaatttaa ttagcacaaa agaaattttg gaattggaaa aggaaatcgg ttatgagttg
    31621 gaagtaaatg aacgcccaac acattcgggg ttgaaccgat tttatgtaag tttcaaaaac
    31681 ggtgaaagta tggagggtgg ttgccttgta agccattgtg gaaacggaaa cactattgat
    31741 gaggcactac aagattattg caagcaaatt agttgccgaa gaatggcatt tggggcttac
    31801 actaatgagc gtaaggaaat tacttttcca aaattagttc atacgaaaat gctcaatcag
    31861 tagggtgcgg tgggaatttt taaaaacatt tcccataacg gtttcgggct tggcgaagtg
    31921 ccgctactcg aaacttaaat tttagcacta actgtcctgc ggcattttgc caattagggt
    31981 gggcattaat aacaataaaa tgaaacttac aaaaagacaa ttattaacag cacaacaagc
    32041 ggttggatat gctgtaatgt atgcaaaaac ggaaagcgaa actgctgaat ttgcattact
    32101 aaaattagat attgatggag cattaataag gttggaagcg gaagaacaaa gaaaagccga
    32161 acacgataaa atgggttgtc cgtttcatta ttgcgacagt aatccacctt gtaaaggcaa
    32221 atgtcattac gcataggtgc ggtgggaaag gtttttaaaa tttaccataa cggtggtgct
    32281 atgcgttcgg ttttgcttgc agaaactatc aattaagcac gaaactatat gcaaaactga
    32341 cgtatagcac gtgttagcgt ttcgtgcttt atttcagatt attaactttt aaaaaataga
    32401 ttatatgaaa ccagtagaat ttaaacatca aaacattgta ttcgcaaaag accaaccaga
    32461 ataccaacca ttaccagcat taagaatcga cagcccgact ggtgaggtta ttagttgctg
    32521 gaaactctcg tttaaagaac gtttgaaaat cgtatttaca ggacgtgttt ggctatctct
    32581 catgagcttt aataaaccac ttaccccaag ttatttagct gtaaatcgaa aagaagtgta
    32641 ttcacatcca gacgatagtg taacattatt ttcaaagcta aaaatgatat ttcggagacg
    32701 gttttagcat gaacgctaac atagatgtta ctgccgctca tcctatacca atagatttag
    32761 attggcaaag aaataagtgt actgaatcag aatccaaagt aatgtttgaa cgtcttgccg
    32821 tactgctact aaccaaaaca attaatcagt aaaaccaatc aaccatgaca accgagttaa
    32881 aatatgaatt ttgtaaaata gataaaacgc attgttatta tctgcctgtg ccggacggtg
    32941 ctttttatgg ttgggtatgg tttcatatcg g
//
LOCUS       DAOQGF010000029        31028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_658_length_31028_cov_6.248119, whole genome shotgun sequence.
ACCESSION   DAOQGF010000029 DAOQGF010000000
VERSION     DAOQGF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_658_length_31028_cov_6.248119"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PK937_06090"
     CDS             complement(<1..145)
                     /locus_tag="PK937_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNH32173.1"
                     /translation="MLVVTTNNIEGKSIKTYYGVVGGEAILGANIFRDFFAGIRDIVG
                     GRSA"
     gene            complement(157..339)
                     /locus_tag="PK937_06095"
     CDS             complement(157..339)
                     /locus_tag="PK937_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32174.1"
                     /translation="MTYSEMKKMCEDIDYYAGHKLKPDDAYEFKKLYNRIKDDEDLDS
                     LSQEKLRKIHDTYLKK"
     gene            complement(342..752)
                     /locus_tag="PK937_06100"
     CDS             complement(342..752)
                     /locus_tag="PK937_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNH32175.1"
                     /translation="MFSFFQRHSHIAVLFLRIGTGFVFIYHGYNKIFAPGGIERFTGY
                     LSGLGFPLPSVLAYTSGGVEFFGGCMLALGLLTRQASLLLIVNMLVAVFIAHRHDTYP
                     QKEHAIQMLVLSIGTLYSGSGAFSLENLVKKHGD"
     gene            complement(771..1724)
                     /locus_tag="PK937_06105"
     CDS             complement(771..1724)
                     /locus_tag="PK937_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32176.1"
                     /translation="MKNLIYVVVVCLVFVSEESVYTQTSKSDDKKTDMVNVCLKSGTK
                     KVPRHSNITIESSSGDEISGELLDCSSEQIVVRSSFGEVAIPLQNVKDVAFAATVNTK
                     GNAQQWPADPNARRALFLPTTETLRKGDKMYENYYLFVHNFYYGVSDQVTINAGLTAI
                     PTDGDFIDNQVYGLGIKARFYETPKIRFAAGAQFVKMPWSESEDVNKFAYTYGVLGIG
                     DPSDAQLNLSMGYILGISSSEKPFIYSGNVSKRLSRNFSLIGEVMWVGTQQDPDYSTV
                     FYSYGIRFMTRRLGVDLGFFNVSEEGYFPGIPMVNFNYSWR"
     gene            complement(1795..3129)
                     /locus_tag="PK937_06110"
     CDS             complement(1795..3129)
                     /locus_tag="PK937_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32177.1"
                     /translation="MKLSWFILLITVCISNVNAQEAQSYFDDGGKMAVIDAATNTRLN
                     LFPSYEGFIEARLFETEDGEYMLEILYQANHKILRQRIPQNGDQVRALRERIASGKTQ
                     QQASVINQEGRVRLLSVAAGASLGYYAIAVPIIAKVEGRAAVGLYLLTGGLGFYVPYQ
                     ITKNMSITHGAADGYTYGLFHGAGYGAALNFLASGGDITGRQFLFSTSVGSIAGSILG
                     YQYAKRNNLSSGDVAVYNIGGLYGTGMGLGAAALTETKKSRIYAASGLVGNMAGLVIG
                     HSFLNAQHYTSGDMDMVMNSGALGAYLLPSVLLPTKLKDNRIYIAATMTGGTLGLMTG
                     NNLIRGKDFTASQSRVIALGGAAGLLTGAGIAYLVAPEDKPRWYIASSAVGGLIGFSW
                     MYASNKDKVEHESGNKTSLKIRFMPENYLMASKMKNKKDAPALPVMSAELRF"
     assembly_gap    3270..3369
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3370..4718)
                     /locus_tag="PK937_06115"
     CDS             complement(<3370..4718)
                     /locus_tag="PK937_06115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH32178.1"
                     /translation="MSDIQRDVQEMDVVFVGAGPSNLAAAYHLSRLIKEHNEAVAAGK
                     KQGQKLEIMAAIVEKGKNVGDHMLSGAILDPSAIRELMPDFKEKGFPIESEVSGESVY
                     LLSESGKIKFPIIPPTLQNHGTYIVSLNKTADWLKTQIESEFGDSVFILTEQTGREML
                     YDGDKVIGVRLGDKNVDHHGKPKSNFEPGADIHAKVTVLGEGPRGSLTKQLVPRLKLN
                     EDRNPQVYGTGVKEIWELPAGRLKKGMVIHTAGWPLPSNHYGGSWLYMMSDTTCSIGF
                     VSALDYSNPSFDPHGAFTKFKTHPYIAGLLEGGKMVSYGAKTVSEGGYWSMPKLYADG
                     VVLVGESGGFLNISRLKGIHLSMKSGMLAAEAIFEALLKNDFSSQSLKRYDDLFRASW
                     AYKEMWDARNWRQNFTGSFYSGMMKAGLQIYLTGGGTKFRVPLHEDYKHMKKISEAGA
                     "
     gene            complement(4746..6530)
                     /locus_tag="PK937_06120"
     CDS             complement(4746..6530)
                     /locus_tag="PK937_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32179.1"
                     /translation="MRLRYYLITFTMISAAAWAQHKTMPETLFEGRYVMVLLNNRDSA
                     QFIKAQEMVNDNDGHIIHAFLPTIIQGYLTRRGEEVLCQSGLVREIVDSVFTGDMTRM
                     SERDRMIVRVWNQQFKPLLATRGQEEDGDNDRTPREVPGKYQTSEYMMGSVAVGVVFV
                     ESDGSLEKSTENWDDYSKEDMIFQIRKGLDWWAQQGGYRASLTWNYEFKDVTTKYEPI
                     THAYNDDENWVSDAAGKLGYGTGDGRMLCYEYANDLRLRYKTDWAFIVFAVAADNDDD
                     GNWKNSEAVAWANLGGPLLVINNRCDGWGPERAWEVMAHETGHIFNALDEYPGGSRGN
                     SRTGLLNVINGNAADGGIINEECIMKAHGDRACDFTRGQIGWIDENRDGVFVSDYLNI
                     SSRFNKKMMLAEAKKITPSDLKIDFEDPRVRMEMERNKKFYSDDFKTNTRGWNEDGAS
                     YIRNGEYSITNTTNWLQDEYSDVEVRVRVRWESGSVSDAFGMDMYSRTTNQFYTCVIN
                     QRGEVMAMQIKAGEGYYPEWKKVASLQKTGINELAMRCEKNTITMYVNGAQVASYTDD
                     GVDQRGVGLRADEGVRAAFDDLEIKKIK"
     gene            6670..7506
                     /locus_tag="PK937_06125"
     CDS             6670..7506
                     /locus_tag="PK937_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH32180.1"
                     /translation="MNNMSLNIHSTLRLYNDVNIPCLGLGVWRTGPGDETRKAVDFAL
                     RSGYRHIDTAHFYKNEKDVGVALKQSGLDRKNVFITTKLWNDDHGYDRALRAFHESLS
                     LLNLTYVDLYLIHYPVSGKRSESWKALEKLYEEGLCRAIGVSNYTQRHLDELLQSARI
                     KPMVNQVEFHPWLYQNELLNFCKTNGIVLEAYSPLTKGQKINAPELSKIAAVYQRSPV
                     QILIRWALQHGIVVLPKSAQPARITENASVFDFNITDQDMARLDAFNCNLRVAWDPTN
                     TP"
     gene            7516..7974
                     /locus_tag="PK937_06130"
     CDS             7516..7974
                     /locus_tag="PK937_06130"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNH32181.1"
                     /translation="MHKLIMLVLLLFVPLRDHRSEYEALHWLLDAWQYTKGSVTFIEN
                     WSHAAPQLFTAHNVTLKNNDTITYEDVRLEIVNDTLCYHVKPKGQALTAFMLVQLTDN
                     KAVFENFTNDFPQRITYHHTSADSLHAYIEGPSRGAWRQIDYYYARITKN"
     gene            7990..8346
                     /locus_tag="PK937_06135"
     CDS             7990..8346
                     /locus_tag="PK937_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNH32182.1"
                     /translation="MKRIFYLASCSTCQKILKTVKPGKDVVLQNIKEETITPDQLDAM
                     KKLAGSYEALFSKRSMKFRAWNLHEKELTEKEMRDLILKEYTFLKRPVAVINDQIFVG
                     NTKIAVDGLKHALKEK"
     gene            8349..8645
                     /locus_tag="PK937_06140"
     CDS             8349..8645
                     /locus_tag="PK937_06140"
                     /inference="COORDINATES: protein motif:HMM:NF040323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HNH32183.1"
                     /translation="MLKQAIPLLHVSDVRQAEAFYCGVLGFEMYSSTRAREDMENPCY
                     MVVWRDNVVLHLSSFSGDGKSGGVVNILVENVDRLYDEFLQKNVSLDLQPTDQT"
     gene            complement(8715..9284)
                     /locus_tag="PK937_06145"
     CDS             complement(8715..9284)
                     /locus_tag="PK937_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32184.1"
                     /translation="MKKFIKENWGRFVFELITIIAGVLIALAVNEVRQNYAQQELADT
                     ALKSIVAEIESNKKFIEQRLPYHRRMVHILDSLALKNADRPFREVGRLAEWRGIQPPI
                     LSDAAWQTAIQSGALAKSDFEKATQIAKLYSFQNAYIRWGDNYIAAVIGGDLASVEQY
                     KNLFYEMGTTGGELVLFYASILNAIQTNK"
     gene            9439..10767
                     /locus_tag="PK937_06150"
     CDS             9439..10767
                     /locus_tag="PK937_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNH32185.1"
                     /translation="MARIRTLIMGAAGRDFHNFNVIYRDNKAYEVIAFTANQIPNIDG
                     RKYPSALAGKLYPKGIPIFPQKDLDALIRKYRIDEVVFSYSDVSYNYLMSCSSIANAA
                     GAHFKILGADPTMIRSKRPVISICAVRTGSGKSQTTRRVSSILKSFGLKVAAIRHPMP
                     YGDLAKQRVQRFAQLADLKKHKCTIEEIEEYEPHIVTGTIIYAGVDYEAILRQAEKEA
                     DVILWDGGNNDMPFYKPDLHIVVADPLRAGHELLYYPSEANLRMADVVIINKEDSADM
                     KSTNLLRDHIHQVNAKAVIVDAASPIFVEHPEKIIGKRVLVVEDGPTLTHGEMKFGAG
                     TVAAQKYGARMIVDPRPYTSGSITETYKKYPDIGVLLPAMGYGSKQVKDLETTINRTP
                     CDTVIIGTPIDLHRIINIKKPSVRVRYDLQEIGTPNLDTLLQKFVKRMKL"
     gene            10840..11364
                     /gene="mog"
                     /locus_tag="PK937_06155"
     CDS             10840..11364
                     /gene="mog"
                     /locus_tag="PK937_06155"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNH32186.1"
                     /translation="MIKIGILTASDRASAGVYEDLSGKAIMETLRVYLRSEWMPVYKV
                     IPDDRASIEKELADMADREECCLIVTTGGTGPAPRDVTPEATEAVCDKMMPGFGELMR
                     AVSLQYVPTAILSRQTAGIRGKTLIVNLPGKPKSIRECLDAVFPAVPYCIELIGGPKL
                     ECHANAVKAFRPKE"
     gene            complement(11386..12630)
                     /locus_tag="PK937_06160"
     CDS             complement(11386..12630)
                     /locus_tag="PK937_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32187.1"
                     /translation="MRKILILVYSFVFTAYVYTQEEADARAIGMALAQTAVSENTDAY
                     RWNPANLGLNYKLKPKLTIAYTGFGFNNRNNIFSRTDYNKYNGRVLSEADKADIRDLL
                     KGKKAFDFNGSGAARALSVQYKNYAVNFEAGGNGNMQLPREILDVVFAKDAYEVGSEN
                     LSARGKGGGMAALRTSLSAGFPLTGLFDKHIREIAVGATLTYMRGIKQVTIKDFRAFI
                     AESDSFITGARFHTLQSSGGNGFSLSFGVSAQINKEWFAGISVIDVYSKMQWKTLNEK
                     RVAQFDVRSEDIFNAKKKSVDTTIYGGQYSTRLPGMLRAGVGYQLDKEWLLAGDIEVI
                     LHNAPGNVTPRIAVGAENHRWRKVILRSGLSVGGVNQGLDMALGAGFLMGKTILDVGT
                     NNLEGMISQRSFSFALQIKTVF"
     gene            complement(12627..14735)
                     /locus_tag="PK937_06165"
     CDS             complement(12627..14735)
                     /locus_tag="PK937_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32188.1"
                     /translation="MPKTFYTYTLMWVSLVSGFTFFSACEKPVEPQFTSALRFPLSKD
                     IYDIFDLKKDIENGKDSLAVDIISDSVVIRIFDRETIHAGDELNVDPTSDSADAAINN
                     DLKVRDSVDINLRLGDLAPGFWSGLHGTNAVIPAFNMPGSYTPTSRNTTQFTQFKSAT
                     LYSGNVALRFTNNTSCAMDSLNVVIFNSTSRTRIDSFVIKNIPATLGSVSQKSITYPA
                     PSSKVVRDSVTIRIAGYSSGSGSSELVDSTAALAIHIVSDITASSVTGKFPSQTISNT
                     DSIVSDSQSDIDSAYIERGSIRLNIRPLGLSVNASVTFTMYDFVDNNGVPLTRSFNII
                     AGQNSTQILDLKNYRLIPNWSVSSSRFVDYRYTIVTVASTNNNTVISNGNGVRVVAVV
                     DSLHFSRMRGTLSNEDFTISNRSQDVNIRDLDSIQLRSAFFEIITSHRIPFPVAMSMT
                     LTGKRGTRSASTVINGSLNKYTSGTDPQRDTLRTPLAEYDEVAALLNLVPDSIYVSGG
                     AIVGDNVANGVITKQDSIDVRINLRAPLVFKLPNDSTKNRIRPTPHVFDVGEGTRKRI
                     YSNLESVRITGTIKNHFPVPVSVQFIIDSTADAARFYDSTNAYKLVYPSSPLIIGAGQ
                     VNSSTGLVTAPTTTTLDYALNKTEFQRLFAPEITKRFRGIKAKLMQTPGTDFVKVSSA
                     DYVDLDTEIEIEVQIDENLQ"
     gene            complement(14778..15161)
                     /locus_tag="PK937_06170"
     CDS             complement(14778..15161)
                     /locus_tag="PK937_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32189.1"
                     /translation="MSGKPKSILYIEDNELNRLLVRRLLEHAGFEVFEAGDGAKGVKA
                     AEELKPDLILMDINLPIMDGFEATRQIKSNPELKHIPIIAITSNAMQGDRERTLAAGC
                     DAYIRKPIDPDAFPGQIAHFIEVFS"
     gene            complement(15165..16799)
                     /locus_tag="PK937_06175"
     CDS             complement(15165..16799)
                     /locus_tag="PK937_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32190.1"
                     /translation="MTHIPHSGDITIIEYDLTPKAASRKKPAPTGDVSIEGLINENVM
                     AEDVRSETEFKKKKKTRKKSSAKNADDTNAGIELEKIVYSSEHLTTIVDHLTDLATEI
                     EHQRLPDTRSPEVTTRCFQLRSCPDHDCVAYQASHARCWYIAGEMCGCRTHLGLPSCF
                     ECQVFQAAATEPLARMRETYLYVLGMLRRRNEKLRTMQRESDLLKERINQVRRIQSSE
                     QLSELDEVFFEQRLVDELVKFSRRTDEFETERDKKLFEQHKILTEQLGSAYLQLQSVT
                     SELERSNKQLEDKVTERTDDLRKSNMALREAVKHSQEADRLKSEFIANVSHELRTPLN
                     SIIGFSKLIMAGVDGPITERQGTDLNAIYNSGKHLLEIINSILELSKIEAGRTDLDIK
                     SFTLEPMVDEIIAATQSLLVGRPLKLEKNIESSLPEIEADMTKIRQVIYNLVSNAVKF
                     TEEGAILISAARDGDWVIVRITDTGIGIDKEHIQSIFERFRQVDGSTTRKAGGTGLGL
                     NIAKKFIELHRGQMHVESRPGEGSTFSFKIPIKHTI"
     gene            complement(16802..17734)
                     /locus_tag="PK937_06180"
     CDS             complement(16802..17734)
                     /locus_tag="PK937_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNH32191.1"
                     /translation="MSRTTGKWFCVINPAANRGNGTRCVPEVRRFLSERNIPHEILVT
                     TQSSEAMPYVVERAADHDVIIAAGGDGTVHEVVNGLMQAYAQSGGQPPKAALGVLPIG
                     SGNDFAKMLHMPKELRPALDAIATGSRRLIDIGRISVDGDHARYFDNNVGIGFDAYVN
                     YESIQIKSLRGVLIYLAAVFKSLFKYQHPMAEIRINGETINNKILMMTTGNGSCSGGG
                     FYITPHAKIDDGWLDVCIVDSRSKLKMLLDLPTVMQGKHDGAPGVRMYRTKNVRIASS
                     ELLPIHADGEVVSMDAHTVELEVMPTAIHAIFAH"
     gene            complement(17781..18167)
                     /locus_tag="PK937_06185"
     CDS             complement(17781..18167)
                     /locus_tag="PK937_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNH32192.1"
                     /translation="MPKEFVRTDLAPAPIGPYNQAVIASGKMIFTSGQIALDPKTGQL
                     LIGDVRQQTRLVLENLSAILRAGGSDLNKIIKTSVFLKDMNDFAAMNEVYAEYMSEAA
                     PARSTVEVARLPKDVLVEIDCIALVE"
     gene            complement(18184..18642)
                     /locus_tag="PK937_06190"
     CDS             complement(18184..18642)
                     /locus_tag="PK937_06190"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNH32193.1"
                     /translation="MKHFLSIVMAMTWLGCSKPAPAPQMDSFGKGVTMSEVTPIAQAV
                     AQVGRYKDKEILIESEIKSVCQKKGCWMEIATENQPMRITFENYGFFVPKDAAGRKVK
                     VQGKIEEKEIPQDEARHYLEDAGKSKEEIEKIVGPQKVVTMVASGVAIVK"
     gene            complement(18685..18885)
                     /locus_tag="PK937_06195"
     CDS             complement(18685..18885)
                     /locus_tag="PK937_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32194.1"
                     /translation="MFGLIRLLLVGAVAYAAFRVIRKLWIVTDPKNNPMRSSSSGDKN
                     KEVRTTVFKSEDIQDAKFKDIK"
     gene            complement(18897..19904)
                     /gene="pheS"
                     /locus_tag="PK937_06200"
     CDS             complement(18897..19904)
                     /gene="pheS"
                     /locus_tag="PK937_06200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNH32195.1"
                     /translation="MDFEQIKSQFQSDTDGQILDKAALEQLQTKYLGKKGILTLATEA
                     LKALPKEQKPQFGKMLNEVKQSVEARLAEIKAAVQAAEVDPKDDFDATLPGRHGWVGH
                     NHPLTIVLNDIKQIFYEMGFTIEDGPEVELDYYNFETVNIPKDHPARDMQDTFFINEN
                     VVLRTHTTPVQARTMQRMKPPIRMICPGRVYRKDTPDATHLPFFNQIEGLVVDEHVTF
                     ADLKGTLAAFVHRLFGNDIGVRFRPSFFSFVEPGAEVDINCIFCRGKGCRTCKNSGWM
                     EILGAGMVHPQLFEYAGYEKNKYTGYAFGMGLDRIVMPLYGINDLRLLIENDVRFLEQ
                     F"
     gene            complement(20046..20399)
                     /gene="rplT"
                     /locus_tag="PK937_06205"
     CDS             complement(20046..20399)
                     /gene="rplT"
                     /locus_tag="PK937_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNH32196.1"
                     /translation="MPRSTNNPASKARRKKIFKQAKGAWGKRKNVLRSAIETVEKGMT
                     YATRDRKANKRNFRSLWIIRINAAVREHGMTYSKFVAALKKANVDVNRKMLAELAMND
                     AAAFNKLIEVAKAAN"
     gene            complement(20442..20639)
                     /gene="rpmI"
                     /locus_tag="PK937_06210"
     CDS             complement(20442..20639)
                     /gene="rpmI"
                     /locus_tag="PK937_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNH32197.1"
                     /translation="MPKTKTNSGAKKRFHVTGSGRILRKKATHRHNMSAKSNTRKSNQ
                     VGKTEVADSDNHRISHLLGLK"
     gene            complement(20673..21191)
                     /gene="infC"
                     /locus_tag="PK937_06215"
     CDS             complement(20673..21191)
                     /gene="infC"
                     /locus_tag="PK937_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNH32198.1"
                     /translation="MGKEDKIRINEQIRTPKVRLIGPKGEQIGIVDIKEALRRSQEEG
                     QDLLEIVPTADPPVCKILDFGKYRYEIQKREKEARKKQTHTGEVKELRFRPVTDDHDY
                     QFKVKHAVNFLKEGYKVKMVVVYKGREIANKEAGIQLAERMVTDLESVGKLDGEMKFE
                     GRNMVMIFIKNR"
     gene            complement(21220..23154)
                     /gene="thrS"
                     /locus_tag="PK937_06220"
     CDS             complement(21220..23154)
                     /gene="thrS"
                     /locus_tag="PK937_06220"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNH32199.1"
                     /translation="MAQINITFPDNSVRSFESGITPMAVATSISRGLAEKCVVAEVNG
                     KQVDLSTPITGDASLKLFTSDTAEGMQVFWHSSAHVMAQAVKRLWPEAKFEDGPPTEA
                     GFFYDILIPEKISEADFVRIEAEMEKIAKEKLTYTRAELTRGEALEFFKKIGQDFKLD
                     IIERIPEGAPISSYTQGEFTDLCRGPHIPHTGMIKSFKIMSVAGAFYKGDENNVQLQR
                     LRAISFPSKKQLDEYLTMLEEAKKRDHRKLGRELELFMITPMIGGGLPVWLPNGTLLR
                     RTLENFLKDELLRRGYQEVVTPHIGNLNLYRTSGHYPYYADSQFSPIKVEDEEYILKP
                     MNCPHHHQIYASKPRSYRDLPLRLAEFGTVYRYEQSGELSGLSRVRGFTQDDAHIYCT
                     HDQLKNELINAIDLTQLVFKTFGMPVKTRLSFRDPKTPDKYAGSPEMWDQAEREIHEV
                     ADAMKLDYFVGIGDAAFYGPKFDFIVRDALGRQWQLGTVQVDYVMPERFGLEFIGGDG
                     QKHRPVIIHRAPFGSMERFISILIEHFAGNFPVWIAPVQIAVLPISDAHTSYAEQVKI
                     ALEKEKIRCQLDTRNEKIGYKIREAEMKKIPYILVVGEKEMQTQTVAVRRHGKGDQGA
                     AGLAEFIAQVKQQIEDKILD"
     gene            complement(23414..24211)
                     /locus_tag="PK937_06225"
     CDS             complement(23414..24211)
                     /locus_tag="PK937_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HNH32200.1"
                     /translation="MKQHNFFIMGVVFFGLACSPKVDNNKKTTVSRNEYTMAALNWMQ
                     ISGEYRALCYQAFNVARMTLDVELSKKPAKKPAVVVDIDETVLDNSPYQARQIANGEQ
                     YPVGWQSWTDEAKAMAVPGAKEFLNYAVSKGADVFYITNRRPSEAPGTLNNLRSLGFP
                     QADTLHIMFREAESNKEARRRKVSETHSILVLCGDNLNDLSDAYDGRLNPERNQRVDQ
                     MKNEWGHRFIVLPNPLYGDWDGAMFNYNFNLSDAAKDSARKAVLRMN"
     gene            complement(24245..25633)
                     /locus_tag="PK937_06230"
     CDS             complement(24245..25633)
                     /locus_tag="PK937_06230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH32201.1"
                     /translation="MDIQFCGAARSVTGSQHLIRINGQKLLLDCGLFQGRRQEAFERN
                     RTFPFDPASVDAMILSHAHIDHSGNIPNLVKQGFKGTIYCSEPTVDLCSVMLQDSAFI
                     QMRDAEHVNYKREKKDEPPIEPIYDIEDAQQAMRHFLGIGYGQAFEPLPGVRATLYNA
                     GHMFGSAMILLEITHNGRKVRFGFTGDLGRKNLPIIEDPHQLRALDYLICESTYGNKV
                     HDPVKEIHGEFVQVIQDAVARKGKIIIPAFSVERTQEIVYHLNLLYENKIVPEIPVFV
                     DSPLAVNVTEVFRKYPDYYDKDARNLLQSGDNPFSFEGLRYIREVKDSMKLNDINDPC
                     IIISASGMCEAGRILHHLKNNIENSNNTVLIVGYMAEHTLGRKLVDKAETVKIFGDTF
                     SVKARVHKINSFSGHADRTDLLNFTELVGKQASVFLVHGEEAQSVPFAESLKSNGFRH
                     VEIPERLQKITL"
     gene            complement(25655..26350)
                     /locus_tag="PK937_06235"
     CDS             complement(25655..26350)
                     /locus_tag="PK937_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNH32202.1"
                     /translation="MQNKRSALIVGASGLIGQHLLNALLEDRDYDRVIVLVRKPIAFT
                     HAKLSQHVVEFDKLTNAAHWIKAHDVFCTLGTTMRTAGSKQAFYKVDFTYPFECARIA
                     KENGVNNFLVVTAIGAKPSSGVFYSRVKGELENALETLRFRSLSIFRPSFLVGERSER
                     RIGEALGIVVFRTLGFLLVGRWKKYRAIRAQAVAEAMIEMAKHERAGKTIYESDVIQT
                     IFDVLIERRRRAV"
     gene            26475..27299
                     /locus_tag="PK937_06240"
     CDS             26475..27299
                     /locus_tag="PK937_06240"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNH32203.1"
                     /translation="MKTWILFLAAAFSLNAQQISTPRPSPRATVSQTVGVTEMTVSYH
                     RPGVKGRTIWGELVPYGQVWRAGANESTTISFSDDVTIEGQKIPAGLYGIQMIPEANE
                     WIVILTKDAKLWGSFNYKPENDAVRIKVKPTSAEMIERLRYSFEDVNDNSTVLTMQWE
                     KLKIAFKIETDTPNLTFTKARAAMNWSGLMQAAAYCLDNNTNLDEGMRWIDLSIGMNE
                     NYYNLRTKAQFVDKTGKRADAIKVMERAMELSKKMPSPPFDIKRMEEVLNEWKKKK"
     gene            27314..28354
                     /locus_tag="PK937_06245"
     CDS             27314..28354
                     /locus_tag="PK937_06245"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNH32204.1"
                     /translation="MKKNFFYVFIAIAGIITAHAQQRLKTPDASPRAVVEQTVGMTEI
                     RVTYYRPAVHERIIWGDLVPYGEVWRAGANENTTVSFSTPVIIGDREIPPGTYGLHMI
                     PMRDKWTIILNRDHKAWGSFFYNENHDVIRFNVEPENSAFQERLLYTFDGIESDRVTL
                     ALKWEKIRIPIPIRVDLHATVLAGFRDELTNVSGFFPEPYVQAVNYCVRNKINYDEAL
                     RWIDHSIELQESFANLNAKSRLLAAMGKNKEADSLNARAMYLATEVDLNNLGYSLMGE
                     NNFKEALAIFQKNAKEHPTSWNVFDSLGELYERMNDKKAAIENYRKALSLVKDGRNKQ
                     RLSETIKKLSGR"
     gene            complement(28424..30124)
                     /locus_tag="PK937_06250"
     CDS             complement(28424..30124)
                     /locus_tag="PK937_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNH32205.1"
                     /translation="MTALDWTVLAVSLIFIIAYGIYKSRGVATMDSYVLSNRDMRWWT
                     VAISIMATQASAITFISTPGQGYADGMRFVQFYFGLPLAMVLLCITAVPFYRRLQVYT
                     AYEFLEKQFDLKTRALASFLFLIQRGLGAGLTIYAPSLILSLLLGWNLVLTNLLIGGL
                     VIIYTTTGGARAVNWTQFHQMMIILTGMAAAFVTVIALLPDGISVNDALHLAGSQGKM
                     NTVDFTWDWDNRYTLWSGLIGGFFLQLSYFGTDQSQVGRYLTGQSIAQTRLGLLTNGL
                     LKIPMQFLILLLGVMVFAFYQFTMPPVFFNRLETERIYQSSYAADYVRLENDHTGLFK
                     RQQEILHVWNAAKESGDEMRMEEQETSLREINKQMAAVKEKAKDLIKAHDPSANTNDT
                     NYIFLTFVLDYLPVGLVGLIIAAIFAASMSSTSAELNALASTTVVDIYRRMFRSDASD
                     AHYVRVSKWATVFWGAFAVGFAELAGQLGSLIEAVNILGSLFYGTILGIFLLAFYWKK
                     VGGTSAFTAAIIAEVIVFLCYFFTPISFLWYNVIGCALVVGLAWVIYFFRQKTTLFQG
                     "
     gene            complement(30315..>31028)
                     /locus_tag="PK937_06255"
     CDS             complement(30315..>31028)
                     /locus_tag="PK937_06255"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNH32206.1"
                     /translation="AMELKTRVRSAIRMKELNANLEDFDEVILAFSRAVEARDPYTRG
                     HSERVGKYSIKLGVALGVPKGFQDILYKGAILHDIGKIGVRDSVLLKKGKLTAEEYDE
                     IKKHPDIGVKICGPLKSSQPLINIIAYHHERMDGKGYPYKIGGKDIPIEARIVAIADT
                     FDALTSARPYREPLTAIQACHLLEEGIGKHFDEELLPEFIDIALRGELQEVMQDPVQA
                     SPPVQNLTDDFVKELDFLM"
BASE COUNT         8390 a         7545 c         6581 g         8412 t
ORIGIN      
        1 atgccgaacg tccgccgacg atgtcgcgta ttcccgcaaa aaaatcacgg aaaatattcg
       61 cgccgaggat agcttcccct ccgacgacgc cgtaatatgt tttgatggat ttgccttcga
      121 tgttattggt ggttacaacc agcatagtac gcttccttat tttttaagat atgtatcatg
      181 aatttttctc aatttttcct gcgacaaact atccagatct tcgtcatctt tgattcggtt
      241 atacaatttt ttgaattcgt acgcgtcatc aggttttaat ttgtgaccgg cgtaataatc
      301 aatatcttca cacatttttt tcatttcact gtacgtcatg tttagtctcc gtgtttttta
      361 accagattct ctaacgagaa tgcgccgctg ccgctgtaga gcgtaccgat cgaaagaacc
      421 agcatttgta tcgcatgctc cttttgagga taggtatcat ggcgatgtgc tataaaaacg
      481 gcgaccagca tattgacgat cagcaacagg ctggcctggc gtgtaagcaa tcccagcgcc
      541 agcatgcacc ctccaaaaaa ttctaccccg cccgacgtgt aggccaacac agatggcaaa
      601 ggaaaaccca aacctgaaag atatcctgtg aatcgttcga tcccgccggg tgcgaatatt
      661 ttattgtatc catgatagat aaaaacgaaa cctgtcccga tgcgaagaaa taaaacggca
      721 atatgagagt gacgttggaa aaagctaaac atgatgagcc ctgtattttg ttatcgccaa
      781 ctataattaa agttaaccat cggtatcccc ggaaaataac cttcctcaga tacattaaaa
      841 aaaccaagat cgacgccaag gcggcgcgtc ataaaacgaa ttccgtaact atagaatacg
      901 gtagaataat ccggatcctg ctgcgtaccg acccacatca cttcaccgat caatgaaaaa
      961 ttgcgggaaa gacgtttact gacatttccg ctgtagataa acggcttttc agaactcgaa
     1021 attcctaaaa tataacccat gctcagatta agttgcgcat cactaggatc gccaataccc
     1081 aatacaccgt aagtatatgc aaatttattg acatcttccg attcagacca cggcattttt
     1141 acaaattgtg cgcccgcggc aaatcgtatt ttaggcgttt cgtaaaaccg tgctttgatc
     1201 cctaagccat aaacctgatt gtctataaaa tcgccatccg ttggaatcgc agtaagcccc
     1261 gcattgattg ttacctgatc agaaacgccg taataaaaat tatgtacgaa gaggtaatag
     1321 ttttcataca ttttatcgcc tttgcgcaag gtttccgtcg ttggtaaaaa caatgcgcgg
     1381 cgcgcattcg gatcagcggg ccattgctgc gcattacctt tagtgttaac tgtggcggca
     1441 aaagcaacgt ccttcacatt ttgtaaagga atcgctactt ctccaaacga tgaacgtacg
     1501 acgatctgtt cggatgaaca atccaaaagc tcgcctgata tctcatcgcc ggaactgctt
     1561 tcgatggtga tattggaatg acgcggaact ttttttgtac cgctcttcaa acaaacattg
     1621 accatatccg ttttcttatc atcacttttg gacgtttgag tataaactga ttcttctgat
     1681 acaaaaacca gacaaactac caccacataa attaaattct tcataatcat tccctcatat
     1741 ttattttatc tctaaattat acagaaaccc ccattgcgta aattaatgcc ctcctcagaa
     1801 tctcaattca gccgacatca caggtaaagc aggcgcatcc ttcttatttt tcattttact
     1861 cgccatcaga taattttcag gcataaatcg aatttttaac gatgttttat ttccggattc
     1921 gtgttctact ttatctttat ttgaggcata catccatgag aatccgatca aaccaccgac
     1981 agcactggat gcgatatacc aacgcggttt atcctccggg gcaacaagat aggctatgcc
     2041 ggcacctgtc agtaaacccg ctgcaccgcc cagtgcaatt acgcgcgact gagaagccgt
     2101 aaaatccttg ccacggatca gattattgcc cgtcatcaaa ccaagtgttc caccggtcat
     2161 cgttgcagcg atgtagatgc gattatcttt taatttagtc ggcagaagaa cggacggtaa
     2221 aagataggcg cccaaagcac ctgaattcat aaccatatcc atatcgccgg acgtatagtg
     2281 ttgcgcattc aaaaacgaat gaccaataac cagtcctgcc atatttccta ctaaaccgga
     2341 ggctgcgtaa atgcgagatt tttttgtttc agtgagcgcc gcggcgccga gtcccatccc
     2401 cgttccatat aatccgccga tattgtacac ggcgacatcg ccggaagata gattattacg
     2461 tttagcatat tgatacccca atatgcttcc ggctatactg ccgacactgg tcgaaaataa
     2521 aaattgacga cctgtgatat cgcctccgga agctaaaaaa tttaatgcgg ccccgtaacc
     2581 tgcgccatga aacaaaccat aggtataacc atctgccgca ccatgagtga tagacatgtt
     2641 ttttgtaatc tgataaggaa cataaaaacc aaggcccccg gttagtaaat ataatcctac
     2701 cgcggctcgt ccttcaactt tcgcgatgat cggtacagct atggcataat aacccaatga
     2761 agctccggca gcaaccgata acaaacgcac gcgcccttcc tgattgataa ccgacgcttg
     2821 ttgttgagtt ttacctgagg caatacgctc acggagcgca cgcacctgat ccccattttg
     2881 aggaatgcgt tgacgcagta ttttatggtt ggcctgatac aaaatctcaa gcatgtattc
     2941 gccgtcttcc gtttcaaata gacgcgcctc aataaaacct tcatacgacg ggaataaatt
     3001 gagcctggta tttgtcgctg cgtcaataac tgccatctta ccgccgtcat caaaatacga
     3061 ctgtgcttcc tgcgcattga cattcgaaat acaaaccgta attaacagga taaaccaact
     3121 taacttcata ctactactat ctgtcatcct tactttttcc cctttttcat aaaaccccca
     3181 ttttttgttt acatattctt ataaacaggc ccgcccccgc cctcgggcgt aacccaattg
     3241 ataacctgat aaggatcggc gatatcgcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnng caccggcttc cgagattttt ttcatgtgct tgtaatcttc atgcagcggg
     3421 acgcggaact tggttccccc gcctgtcaaa tatatctgca atccggcttt catcatgccg
     3481 ctgtaaaaac tgccggtgaa attttgccgc cagttgcgag catcccacat ttctttgtaa
     3541 gcccagcttg cgcggaaaag atcatcataa cgcttgagcg attgagatga aaaatcattt
     3601 ttcagcaacg cttcaaagat cgcctccgcc gccagcatgc ccgatttcat cgagaggtga
     3661 atacccttaa gtctggaaat attcagaaac ccgccggatt cgccgaccaa tactacgccg
     3721 tcggcataca gtttgggcat ggaccaatag ccgccttcag aaaccgtttt agccccgtag
     3781 gaaaccattt taccgccttc gagcaatccg gcaatgtaag gatgggtttt aaatttggta
     3841 aacgcgccat gcggatcaaa cgatggattg ctgtaatcca atgccgaaac gaatcctatc
     3901 gagcacgtcg tatcgctcat catatagagc caacttccgc cgtaatgatt actcggaagc
     3961 ggccatcccg ctgtgtgaat aaccatacct ttcttgagac gaccggcagg caattcccat
     4021 atttctttca ctccggtacc gtacacttgc ggattgcgat cttcattgag tttgaggcgc
     4081 ggtacgagtt gtttggtcaa acttccgcgc gggccctcac cgagcaccgt tactttggca
     4141 tgaatatctg cgccgggttc aaaattggat ttcggtttgc cgtgatgatc cacgttttta
     4201 tcgcccagcc gtacaccgat cactttgtca ccgtcgtaca acatttcacg accggtttgt
     4261 tcggtaagaa taaatacgct atcgccaaac tcgctttcga tctgcgtttt gagccaatca
     4321 gctgttttat taagcgaaac aatgtatgtt ccgtgatttt ggagcgtcgg aggtatgatt
     4381 ggaaatttaa tttttccgga ttctgataat aaataaaccg attcaccact gacttcggat
     4441 tcgatcggaa atcctttttc cttaaaatcc ggcatcagtt cgcgtatcgc cgacggatca
     4501 aggatcgcac cggataacat atgatcgccg acgttttttc ctttttcgac aatagccgcc
     4561 atgatttcca gtttctgtcc ctgttttttt ccggcagcaa cggcttcgtt atgttctttg
     4621 atgagacggg agagatgata cgccgcggcc aaattagacg gaccggcgcc gacaaatact
     4681 acatccattt cctgcacatc gcgttgaata tcagacatac tctaatctcc tcatatcgta
     4741 tgggactatt taattttttt gatttccaga tcgtcaaatg ctgcgcgaac accttcgtct
     4801 gcgcgcaaac cgacgccgcg ctgatccaca ccgtcatccg tgtagctggc aacctgcgcg
     4861 ccattgacgt acatcgttat cgtgtttttt tcgcatcgca tagcaagttc gttgataccg
     4921 gttttctgaa gcgaggctac ttttttccat tcgggataat atccttcgcc ggctttgatc
     4981 tgcatcgcca tgacttcccc gcgttgattg atcacgcagg tataaaactg attggtcgta
     5041 cggctgtaca tatccatgcc aaatgcgtcc gacacggaac cgctctccca ccgcacgcgt
     5101 acgcgtactt cgacgtcact gtattcgtct tgcaaccaat tggtggtatt ggtgatggag
     5161 tactccccgt ttcggatata cgacgcaccg tcttcattcc acccgcgcgt attagtttta
     5221 aaatcatcgc tataaaattt tttgttgcgc tccatctcca tacgaacgcg cggatcttcg
     5281 aaatcaattt tcagatcgga aggcgtgatt tttttggctt ccgcgagcat cattttttta
     5341 ttaaaccgtg acgagatatt gaggtaatcg ctgacaaaaa caccgtctct gttttcatca
     5401 atccatccga tctggccacg tgtaaaatcg cacgcgcgat ccccatgcgc tttcattata
     5461 cattcttcat tgatgatgcc gccgtcggcg gcgttaccgt taataacatt gagcaaacct
     5521 gtacgcgaat tgccgcgtga tccgccgggg tattcgtcca acgcattaaa aatatgcccg
     5581 gtttcatgcg ccatcacttc ccatgcgcgt tccggtcccc atccgtcgca acggttgttg
     5641 atcaccagca aaggcccgcc cagattagcc caggcgacgg cttcgctgtt tttccaatta
     5701 ccgtcatcat cattatcggc cgctacggca aatacgataa acgcccagtc cgtcttgtaa
     5761 cgaaggcgca aatcattggc gtattcataa cagagcatac gcccgtcacc ggtaccgtaa
     5821 cccaatttgc ccgccgcgtc actcacccaa ttttcgtcat cgttatatgc gtgagtaatc
     5881 ggttcgtatt tcgtcgttac gtctttaaat tcataattcc atgtcagact cgcacggtag
     5941 ccgccttgtt gagcccacca gtctagccct ttacggattt gaaatatcat atcttctttg
     6001 gaataatcat cccagttttc cgtacttttt tccagcgatc cgtcgctttc tacaaaaaca
     6061 acaccgacgg ccacggatcc catcatgtat tcactggttt gatattttcc gggcacctcg
     6121 cgtggcgtgc gatcattgtc accgtcctct tcctgccctc gtgtcgccaa gagcggtttg
     6181 aattgttgat tccaaacgcg cacgatcatc cgatcgcgtt ccgacatacg cgtcatatcg
     6241 ccggtgaata ccgagtccac gatttcgcgt accaatccgc tttggcaaag cacttcttca
     6301 ccgcgtcgtg tcagataacc ttggataatg gttggcaaaa acgcgtgtat gatatgaccg
     6361 tcattatcat ttaccatttc ctgcgctttg atgaattgcg cggaatcccg attattgagc
     6421 aacaccatga catatcggcc ttcaaaaagt gtctcgggca tagttttgtg ttgcgcccaa
     6481 gctgcggccg aaatcatcgt aaaagtgatc aaatagtaac gtaacctcat aaaaaacctc
     6541 atattgtgat gttcccaaaa gacgccggaa attattatct ttcaccgcca aaggcaaagt
     6601 aaattttggt attggaatgc accatggccg gcattagatt gatacattac acactcattt
     6661 tgtaaacctt tgaacaacat gtcgctgaat atccattcta ctctacgact gtacaacgat
     6721 gtaaacatac cctgtctcgg tttaggtgtc tggcgaaccg gcccgggtga tgaaacacga
     6781 aaggccgtag attttgcttt gcgcagcggt taccggcaca ttgatacggc tcatttttac
     6841 aaaaacgaaa aagacgtcgg cgttgctttg aaacaatcag ggcttgatcg caagaatgtc
     6901 tttatcacga caaaactgtg gaatgatgat cacgggtacg atcgtgctct tcgtgcgttc
     6961 catgaaagcc tatctttgct caatctgacc tatgttgatt tatatctcat tcactatccg
     7021 gtgagcggca aacgcagtga aagctggaag gctttggaaa aattatacga agaaggtctg
     7081 tgccgcgcca tcggcgtaag caactatacg cagcgtcatt tagatgaact tctgcaatcc
     7141 gcccgcatca aaccgatggt caatcaggta gaatttcatc cctggcttta tcaaaatgaa
     7201 ctgcttaact tttgtaaaac gaacggtatc gtactcgaag cgtatagtcc cttgaccaaa
     7261 ggtcaaaaaa tcaatgctcc ggaactgtct aaaatcgccg cggtatatca acgctcaccc
     7321 gtgcaaatat tgattcggtg ggcgttacag cacggtatcg ttgtattgcc taaatcagcg
     7381 caaccggcgc gcattactga aaatgcatcc gtatttgatt ttaatattac cgatcaagac
     7441 atggcacgac tggatgcatt caactgcaat ttgcgtgtgg cttgggaccc gacaaacacc
     7501 ccctaaggag aaaatatgca caagttaatc atgttggttt tactactttt cgttccctta
     7561 cgcgaccacc gttcagaata cgaagcattg cattggctgc tcgacgcgtg gcaatacacc
     7621 aaaggatcgg tgacatttat cgaaaactgg tcacacgccg caccccagct ttttacggca
     7681 cacaatgtta cgctgaaaaa caacgatacg ataacgtacg aagatgtacg cctcgaaatc
     7741 gtcaacgata ccttatgtta tcatgtcaaa cccaaaggtc aggcattaac ggcttttatg
     7801 ctcgtacagt taacagacaa caaagccgtt tttgaaaatt tcaccaatga cttcccgcaa
     7861 cgcatcacct atcatcatac atcggccgat tcactgcatg cctatatcga aggcccttcc
     7921 cgcggcgctt ggagacaaat agactattac tacgcacgca ttaccaaaaa ctaaaataac
     7981 aatcacatca tgaaacgtat tttttatttg gcatcgtgca gcacttgcca aaaaattctc
     8041 aaaacggtca aacccggtaa agatgtagta ctacaaaata taaaagaaga aaccatcacg
     8101 ccggatcaat tggatgccat gaaaaaactg gccggttcct acgaagcatt attcagcaaa
     8161 cgttccatga aattcagagc atggaatttg catgaaaaag aactgacgga aaaagaaatg
     8221 cgcgacctga ttctcaaaga atacacgttt ctcaaacgcc cggttgccgt tataaacgat
     8281 cagatatttg taggtaatac aaagatagcc gttgacggtt tgaagcatgc attgaaagaa
     8341 aaataaatat gttgaaacaa gctatacctc tgctgcatgt aagcgacgta cgacaggcgg
     8401 aagcgtttta ttgcggcgtg cttggctttg aaatgtactc ttcaacccgt gcaagagagg
     8461 atatggaaaa cccctgttac atggtcgttt ggcgtgataa cgtagtcttg catctgtcgt
     8521 ctttttcggg agacgggaag tccggtggtg tggtgaatat cttagtcgaa aacgtagatc
     8581 gtttgtacga tgagttttta caaaaaaacg tgtccctcga tttacaaccg acggatcaga
     8641 catgaggtac gcgtgagatg tatgtaaaag acaccgacca aaattgcctg cgttttttta
     8701 cattgaaata acgatcattt atttgtttgt atggcattta aaatagacgc ataaaaaagt
     8761 acaagctccc cgccggtagt ccccatttca taaaaaagat ttttatactg ttccacggac
     8821 gcgagatcac ctccgataac tgcggctata taattatcac cccagcggat atacgcattc
     8881 tgaaacgagt ataattttgc aatttgcgtc gctttctcaa aatcggattt agccaaagcg
     8941 ccggactgaa tcgctgtttg ccatgcggca tcgctgagta tcggtggttg tataccgcgc
     9001 cactccgcca aacgtcctac ttcacgaaac gggcgatcgg cattttttaa tgctaatgaa
     9061 tccaatatat gcaccatgcg acgatgatag ggcaatcgct gttcgataaa ctttttattc
     9121 gattcgattt ctgcgacgat acttttcaac gctgtatcgg ccaattcttg ttgggcataa
     9181 ttttgcctta cctcatttac ggctaatgca atgagaaccc ccgcaatgat cgtaattaat
     9241 tcaaaaacaa agcgccccca attttctttg ataaatttct tcattcgata tcctaagcat
     9301 atttatttaa gatagactgc caaatataat gaatcaagca tttagggatt gtttactcat
     9361 atctctgcat gactgagatc atacccgtgt gcattttcct attgtacgtt ataacgttgt
     9421 aaactcggag gttcttttat ggcacgtatt cgaactttaa tcatgggcgc agccggccgt
     9481 gattttcata attttaatgt aatctatcgc gataacaaag catacgaagt cattgctttt
     9541 acggcgaatc aaatacccaa tattgacgga cgcaaatatc cgtcggcatt ggcgggaaaa
     9601 ctctatccga aaggtattcc gatttttccg caaaaagatt tggatgcatt gatacgcaaa
     9661 taccggattg acgaagtcgt tttttcctac agtgatgtct cttacaatta tctgatgtca
     9721 tgcagctcga ttgccaatgc ggcgggcgcg catttcaaaa tcctcggcgc tgatcccacg
     9781 atgattcgca gtaagcgtcc ggtgatttcc atttgcgctg ttcgtaccgg ttctggaaaa
     9841 agtcaaacca cgcggcgtgt ttcttcgatt ctcaaatcgt tcgggcttaa ggtagcggcc
     9901 atccgtcacc ctatgcctta cggtgatttg gccaaacaac gtgtacagcg ttttgcgcag
     9961 cttgcggatc tcaaaaaaca taagtgtaca atcgaagaaa ttgaggaata tgaaccgcac
    10021 atcgtaacgg gcacgatcat ttatgccggc gtggattatg aagcgatttt acgtcaggcc
    10081 gaaaaagaag cggatgtgat tttgtgggac ggcggtaata acgacatgcc gttttataaa
    10141 cccgatctgc atatcgtggt agccgatccg ctgcgtgcag ggcatgaatt gttgtattat
    10201 cccagcgaag ccaatctccg tatggccgat gttgtaatca tcaataaaga agattcggcg
    10261 gatatgaaat ccaccaatct gctgcgtgac catattcatc aagtcaatgc caaagccgtg
    10321 atcgtggatg ccgcttcgcc gatatttgtc gaacatcctg agaagatcat cggaaaacgc
    10381 gtgctggtcg ttgaagacgg cccaacgctc acgcatggtg aaatgaaatt tggcgccggg
    10441 acggtggccg cacaaaaata cggcgcacga atgatcgtgg atccgcgccc ttacacatcc
    10501 gggagtatca ccgaaactta caaaaaatat cccgacatcg gcgtcctgtt gcccgcgatg
    10561 ggctacggta gcaaacaagt caaagacctt gaaacgacga tcaaccgtac cccgtgcgat
    10621 acagtgatca tcggcacgcc gattgattta catcgcatta ttaatatcaa aaagccttcc
    10681 gtgcgtgtgc gatacgacct acaagaaatc ggcacgccca atctcgatac attgttacag
    10741 aaattcgtga aacgcatgaa attataacgt ttcatttttt tctacccttc caatcccgtc
    10801 atgattacct tcctgacggg attttttttg ggagataata tgatcaaaat aggtattctc
    10861 actgcttccg atcgtgccag cgccggtgtg tatgaagatc tttccggtaa agcgattatg
    10921 gaaacattgc gcgtgtatct gcgatccgag tggatgccgg tttataaagt tataccggac
    10981 gacagagcaa gtatcgaaaa agaattggct gatatggcgg atcgcgaaga gtgttgtctg
    11041 atcgtaacga ccggcggaac gggtcctgcc ccgcgcgatg tcacgcctga agcgacagag
    11101 gcggtgtgcg ataagatgat gccgggattt ggtgaattga tgcgtgctgt atcgctgcag
    11161 tatgtcccaa cggccattct ttcacgccaa acggccggca ttcgcggtaa gacgttgatc
    11221 gtcaaccttc ccggaaaacc caaatccatt cgtgaatgtt tagatgccgt atttcctgct
    11281 gtgccttatt gtattgaatt aatcggggga ccaaagttgg aatgtcatgc caatgccgta
    11341 aaagcttttc ggccaaaaga atgactccgt aagaattttg cgattttaaa atacggtttt
    11401 tatttgcagg gcaaaagaga aactgcgttg agaaatcatg ccttcaagat tattcgtgcc
    11461 cacatcaaga attgtttttc ccatcaaaaa accggcaccg agtgccatat ctaaaccttg
    11521 gttgacacca ccgacactga ggccactgcg aagaataacc ttgcgccagc ggtggttttc
    11581 agctccgact gcaatacgag gcgtcacatt tcccggcgca ttatggagta tcacctcaat
    11641 atcgccggct aaaagccatt ctttatccag ttggtagccg actccggcgc gaagcatccc
    11701 cggcaaacgg gtagaatatt gccctccgta aatagtcgta tctacggatt ttttcttggc
    11761 attaaaaata tcctcgctgc gtacatcaaa ctgggccaca cgtttttcat tcagcgtttt
    11821 ccactgcatc ttgctatata catctattac gctgattccg gcaaaccatt ctttattgat
    11881 ctgagcgctg actccaaaac taagggaaaa accattaccg ccggaggact ggagtgtgtg
    11941 aaatcttgct ccggtaataa aactatccga ctctgcaata aaggcgcgaa aatctttgat
    12001 tgtgacttgt tttattccgc gcatatacgt aagcgtggcg ccgacggcta tttcgcgaat
    12061 gtgcttatca aataaaccgg ttagcggaaa acccgccgaa agcgaagtac gcaacgcggc
    12121 catgccgccg cccttgccac gcgcgctaag attttcggaa ccgacttcgt aggcatcttt
    12181 agcaaaaact acatctaaaa tctcacgcgg caattgcata tttccgttgc cacccgcttc
    12241 aaaatttacg gcataatttt tatactgcac actgagtgct cgcgccgcgc cggaaccgtt
    12301 aaaatcaaac gcttttttac ctttcaaaag atcacgaata tccgctttgt cggcttccga
    12361 aagaacgcgc ccgttgtatt tattataatc tgtgcgtgaa aaaatattat ttcgattgtt
    12421 aaagccaaat ccggtgtatg ctatcgtaag tttcggtttg agtttgtaat tcaaaccaag
    12481 gtttgccgga ttccatcggt aagcatccgt attttcggaa acggctgtct gagcgagcgc
    12541 catccctatc gcacgtgcat ccgcctcttc ctgcgtataa acatacgcag tgaaaacaaa
    12601 gctatatact aaaattaata tttttctcat tgtaaatttt catcgatttg aacttcaatt
    12661 tcgatctccg tatccaaatc cacataatcg gccgaactga cttttacaaa atctgtcccg
    12721 ggcgtttgca tgagtttggc tttgataccc cggaaacgtt ttgttatctc cggcgcgaat
    12781 aatcgttgaa attctgtttt attgagggcg taatcaagcg tcgtggttgt cggtgcggta
    12841 accaatccgg ttgacgaatt aacctggccg gcaccgataa tcaatggcga agatggatat
    12901 accagcttat aagcattggt cgaatcataa aaacgagctg catcggcggt gctatcaata
    12961 ataaattgaa ccgacactgg aacggggaaa tgatttttaa tagtaccggt tatacgaacg
    13021 gattccagat tggaataaat tctcttgcgg gtaccttcgc cgacatcaaa cacatgcggt
    13081 gtagggcgta tgcggttttt ggtggagtcg ttgggcagtt taaaaacaag cggagccctc
    13141 agattgattc gcacgtctat ggagtcttgt ttggtaataa ctccgttggc cacattgtcg
    13201 ccgactatcg cgccaccgct gacataaatc gaatccggaa caagattaag aagcgcagcc
    13261 acttcatcat attcggctaa cggtgtacgc aaggtatcac gttgcggatc agttcccgat
    13321 gtgtatttat taagactgcc attgatcacc gtcgaagccg aacgcgttcc gcgtttgccg
    13381 gtcaacgtca ttgacatagc gaccgggaaa ggaatacgat gcgaagtgat gatttcaaaa
    13441 aaagcagaac gtaactgtat gctgtctaaa tcacgaatat tgacatcctg actgcgattg
    13501 gaaatagtaa aatcttcatt agataaggtg ccgcgcatgc gagaaaaatg taatgaatca
    13561 accacggcaa cgacacgtac accgttcccg ttggagataa ctgtattatt attagtagaa
    13621 gcaacggtaa caatcgtgta tctataatcc acgaaacgac tgctactaac ggaccaatta
    13681 ggaataagcc gataattttt caggtcaagt atctgcgtac tattttgccc agcaatgata
    13741 ttaaaacttc tcgttaacgg tacaccgttg ttatctacaa aatcatacat tgtaaaagta
    13801 acggacgcat tgacggacaa ccccagtgga cgaatattga gtcgaatgct gcctctttct
    13861 atgtatgccg aatctatatc cgactggcta tcggaaacaa tagaatccgt attactgata
    13921 gtttgcgacg gaaatttacc ggtaaccgat gaggccgtga tatcagaaac aatgtgaatg
    13981 gctaacgcag cggtcgaatc aaccagttca gagcttcccg aaccactgga ataaccggct
    14041 atacgaatcg tcaccgaatc ccgtacaact ttggatgaag gcgcaggata cgtgatactt
    14101 ttttgggata cgctgcctaa agtagccgga atatttttga tgacaaaact gtcgatacgt
    14161 gtacgcgatg tcgaattaaa tattaccacg ttcagcgagt ccattgcgca acttgtatta
    14221 ttggtaaatc gtaaagctac gtttccgcta taaagcgtcg ctgatttaaa ctgagtaaac
    14281 tgcgttgtat tgcgcgatgt aggagtgtaa ctgcccggca tattgaaagc cggaataact
    14341 gcattcgtac catgcagacc tgaccaaaaa cccggtgcca aatcgcccaa gcgcaaattg
    14401 atatcaacgg aatcgcgaac cttgagatca ttattgatcg ccgcgtcagc cgaatccgat
    14461 gtaggatcca cattcaattc gtctccggca tgtatggttt ctctgtcaaa aatacgaatc
    14521 actactgaat cgctgataat atccacggcc agcgaatctt ttccattttc gatatctttt
    14581 ttcaaatcaa aaatatcgta gatgtctttg ctcagaggaa aacgtaacgc ggaagtaaat
    14641 tgaggctcaa cgggtttttc gcatgcagag aaaaaggtaa accccgacac aaggctcacc
    14701 cacatcaacg tgtaggtata aaatgttttt ggcatatttt ttaaatataa aaaaaaggcg
    14761 cacgtgcgca agagcaatca ggaaaaaact tcgataaaat gcgcaatttg cccgggaaaa
    14821 gcgtccggat cgatcggttt acgaatgtac gcgtcacaac ctgcagccaa tgtgcgttca
    14881 cgatcgcctt gcatggcgtt ggacgtaata gctatgatag gaatatgttt taattcagga
    14941 ttagatttaa tttggcgtgt ggcttcaaat ccgtccatga tcggcaaatt aatatccatc
    15001 aaaatcagat cgggtttcaa ttcctccgct gctttgacac ctttggcccc atcaccggct
    15061 tcgaaaactt caaaccctgc atgttcgagt aacctgcgca ccagtaaacg attgagttcg
    15121 ttgtcttcga tgtataaaat gcttttgggt ttaccgctca tatgtcagat tgtatgtttt
    15181 atcggtattt taaacgaaaa cgtactgcct tcgcccggac gactttcgac atgcatctga
    15241 ccgcgatgca attcgataaa ttttttagca atattcagcc ccaaacccgt tccgcccgct
    15301 ttgcgcgtgg ttgaaccgtc tacctgacga aatcgctcga aaatagattg gatatgttct
    15361 ttatcaatgc cgatgccggt atctgtgatg cgtacaatga cccagtcgcc atcgcgagcc
    15421 gcgctgatca gtatggcccc ctcttcagta aatttaaccg cattagaaac cagattatag
    15481 atcacctgac gtatcttcgt catatcggct tcgatttccg gcaacgacga ttcaatattt
    15541 ttctcaagtt tcagcggacg cccgaccagc agtgattgcg tagcggcaat aatttcatcc
    15601 accataggtt ccagcgtaaa cgacttgata tccagatccg tacgaccggc ctcgattttg
    15661 gaaagctcaa ggatgctatt gataatctcc agtaaatgtt taccgctgtt ataaatagca
    15721 ttcaaatcgg taccctggcg ttccgttatc ggcccgtcaa caccggccat gattaacttg
    15781 gaaaaaccga taatcgaatt aagaggtgta cgcaattcgt ggctcacatt agcaataaat
    15841 tccgatttca aacgatcggc ttcctgcgaa tgtttgacgg cttcgcgcaa agccatgttg
    15901 ctcttacgga gatcgtccgt acgctccgtt actttatctt ccaattgttt attggatcgc
    15961 tccagctcgg acgtcacaga ttgcaattgc agataggcgc tgcctaattg ttccgtgagt
    16021 attttatgct gttcaaatag ttttttatcc cgttccgttt caaactcgtc cgtgcgccgc
    16081 gagaatttaa ccaactcatc aaccagtctt tgttcaaaaa acacctcatc caactccgac
    16141 agttgttcac tgctttgaat acgccgaacc tgattgatgc gttctttgag caagtccgac
    16201 tcgcgctgca tggtgcgtaa tttttcattg cggcgacgta acatgccgag cacatacaga
    16261 taagtttcac gcatccttgc cagcggttcg gttgcggccg cctgaaaaac ctggcattcg
    16321 aagcatgacg gcaatcccag atgagtgcgg caaccgcaca tttcacccgc gatataccag
    16381 cagcgggcat gcgaagcctg ataggctacg caatcatggt ccgggcatga acgcagttga
    16441 aagcaacggg ttgttacctc cggtgagcgt gtatccggta aacgttgatg ttcgatctcc
    16501 gttgcgaggt cagtcaagtg atcgacgatc gtcgtcagat gttcgctgga ataaacaatt
    16561 ttttcaagtt cgatgccggc attagtatca tctgcatttt tagccgaact tttttttcgc
    16621 gtcttttttt tctttttaaa ttcagtttcg ctgcgtacgt cttccgccat tacgttttca
    16681 ttgatcaatc cttcgatgga tacatcaccg gttggcgcag gctttttacg tgaagctgct
    16741 ttaggcgtca gatcgtattc gatgatggta atatcgccgc tgtgcggaat gtgtgtcata
    16801 atcagtgcgc aaatattgcg tgaatagccg tcggcattac ttctaattcg acggtgtggg
    16861 cgtccataga tacgacttcc ccatcggcat ggatcggcag taactcagac gacgcaatgc
    16921 gaacattttt ggtacggtac atacgaacgc ccggcgcccc atcatgttta ccttgcatga
    16981 ctgtgggcaa gtccaacagc atttttaatt tgctgcggga atccacaata catacatcca
    17041 accatccgtc gtcaattttg gcatggggtg tgatataaaa tccgccgccg gagcatgaac
    17101 cgttgcccgt cgtcatcatt agaattttgt tgtttatcgt ttcgccattg attcgtattt
    17161 ccgccatcgg atgctggtat ttaaaaagcg atttgaatac ggccgcaaga taaatcaaaa
    17221 cgccgcgcag cgatttgatc tgaatgcttt cataattgac atatgcatca aagccgatgc
    17281 cgacattatt gtcgaaatac cgtgcatgat cgccgtccac gctgatacga cctatatcga
    17341 tcaagcgccg cgatcccgtt gcgatcgcgt ccaacgccgg acgaagttct ttaggcatat
    17401 gaagcatttt cgcaaaatca ttaccggatc cgatcggaag tacgcccagt gcggctttgg
    17461 gaggctgacc gcccgactgc gcataagcct gcatcaatcc gttgacaact tcgtgtaccg
    17521 taccatcccc gcccgcggca atgatcacat cgtgatccgc tgcgcgctcg accacatacg
    17581 gcatcgcttc actggattgg gtggtcacca atatttcatg cgggatattt cgttccgaca
    17641 gaaagcgccg cacttccggt acgcatcgcg taccgttgcc gcggttagcc gccggattga
    17701 tgacgcaaaa ccacttgccg gttgtcctac tcatacaccg tcaccgtttg tttagtgaaa
    17761 acgtctattt aaaatagtgc ttattcgacg agcgctatac aatcaatttc caccagcaca
    17821 tctttgggca ggcgtgcgac ttctacggtg gatcgtgccg gtgcggcttc cgacatatat
    17881 tctgcatata cttcattcat tgccgcaaaa tcattcatat ctttgagaaa tactgatgtt
    17941 ttgatgattt tattcagatc gcttcctccg gcgcgaagta tcgcgctaag gttttcaagc
    18001 accaggcgtg tttgctggcg tacatcgccg attaatagct gtccggtttt gggatcaaga
    18061 gcaatctgtc cggaggtgaa tatcatttta ccggaggcaa tgacagcctg attgtaaggc
    18121 ccaataggag ccggcgccag atctgttctt acaaattctt tgggcatatt ccctcgttaa
    18181 taattatttt acaatcgcta caccggaggc taccatcgtt accacttttt gagggccgac
    18241 gattttttca atttcttctt tggatttacc cgcatcctcc agataatggc gtgcctcgtc
    18301 ttgcggaatt tctttctctt cgattttgcc ctgtactttt actttacgac cggcggcatc
    18361 tttaggaaca aaaaaaccat agttttcaaa tgtgatgcgc atcggttggt tttccgttgc
    18421 gatctccatc cagcagcctt ttttctgaca aaccgatttg atctcggatt cgatcagtat
    18481 ttctttatct ttgtagcggc caacttgtgc caccgcttgg gcgatcggcg ttacttccga
    18541 catggtcaca cctttgccaa aagaatccat ttgcggtgcc ggtgcaggtt tggaacaacc
    18601 taaccaagtc atggccatca ctatgctgag aaaatgcttc atttgttttc cttctttaaa
    18661 ctattataga gttagaaatt aatcttactt aatgtctttg aatttagcgt cttgtatatc
    18721 ttcagacttg aagaccgtcg ttcgcacttc tttattttta tcacccgaag aggaactgcg
    18781 catcggatta ttcttaggat ccgtgacaat ccataattta cgaataacac gaaaggccgc
    18841 atacgcgacg gcgccgacca ataacaatcg tattaaacca aacataaaaa tccttattag
    18901 aattgttcga gaaaacgcac atcgttttct atcaaaagtc ttaaatcatt gattccgtac
    18961 aacggcatga cgatacgatc caagcccata ccgaaggcat agccggtata tttatttttt
    19021 tcataaccgg cgtattcaaa taattgcgga tgcaccatgc cggcgcccag aatttccatc
    19081 catccggaat ttttacaagt acggcagcct ttaccgcggc aaaatataca gttgatatcc
    19141 acctcggctc cgggttcaac gaacgaaaaa aatgacggtc gaaagcgaac gccgatatca
    19201 ttgccaaaca aacgatgaac aaacgcggca agcgttcctt tcaagtcagc aaacgtgaca
    19261 tgttcatcca ctaccagccc ttcgatttga ttaaaaaaag gaagatgcgt ggcgtcgggc
    19321 gtatcttttc ggtaaacgcg gccgggacag atcattcgga tcggcggctt catccgttgc
    19381 atggtgcgcg cctgaacagg tgtcgtatgc gtacgcaaaa ccacattttc gttgataaaa
    19441 aaagtatcct gcatatcgcg tgcaggatgg tctttgggaa tattcaccgt ttcaaaattg
    19501 tagtaatcca attcgacttc cggaccgtct tcgatggtaa atcccatctc gtaaaaaatc
    19561 tgcttgatgt cattgagaac gatcgtcaaa ggatgattgt gccctaccca tccgtgacgc
    19621 cccggtaatg tggcatcaaa atcatccttc ggatcaactt ccgccgcttg cacggccgct
    19681 ttgatttcgg ccaaccgcgc ctcaacactt tgtttgactt cgttgagcat tttaccgaat
    19741 tggggctttt gctctttcgg cagcgccttg agcgcttcgg tagccagcgt cagaatacct
    19801 ttttttccaa gatattttgt ttgcagttgt tcgagtgcgg ctttatccaa tatttgcccg
    19861 tcggtatcgc tttgaaactg cgattttatc tgttcgaaat ccatgacttc cttcttcaaa
    19921 aattcatcat tcacatggta tcaaaaaaaa cacgtattcg caagattttt gccgtcctgt
    19981 gaagcaaaaa aaagacatgc ctcatagtgt ggcatgtctt ttatacattc gtctgttatg
    20041 cgaaattagt tcgctgcttt agctacttca ataagtttat tgaatgccgc tgcatcgttc
    20101 atggccaatt cggccagcat tttacgattc acgtcaacat tggctttttt caacgcggca
    20161 acgaatttgg aatacgtcat gccgtgttca cggacagccg cattgatacg aataatccac
    20221 aagctgcgga aattacgttt gttggcttta cgatcgcgcg ttgcataggt cattcctttt
    20281 tcgacggttt cgatcgcgct acgcaataca tttttgcgtt tgccccaggc gcctttggcc
    20341 tgcttaaaaa tctttttccg gcgggcctta ctggccgggt tatttgtgga acgtggcatg
    20401 gtattctcct tttaaatttt tttctttaat tacgcctgca attacttcaa gccaagcaga
    20461 tggcttatgc gatgattatc cgagtccgcc acttccgttt tgccgacctg attggatttg
    20521 cgggtattgg atttggcgct catattgtgg cgatgggttg ctttcttacg gagaatacgg
    20581 cctgagccgg tcacatgaaa gcgcttcttt gcgccgctgt tggttttagt ttttggcatg
    20641 ggatgctcct tattagatta atttattttg tattagcgat ttttgataaa gatcataacc
    20701 atattacgac cttcaaattt catttcaccg tcgagtttgc caacagactc cagatccgtc
    20761 accatacgct cggccaattg aataccggcc tctttgttgg caatttcacg gcccttatac
    20821 acaacgacca ttttgacctt atatccctct ttaagaaaat tgacagcgtg tttcacctta
    20881 aactggtagt cgtgatcgtc cgtgacaggt cggaaacgca attctttgac ttcgcccgta
    20941 tgggtttgtt ttttacgcgc ttctttttca cgcttttgga tttcataacg atattttcca
    21001 aaatccaaaa ttttacaaac cggcggatca gccgtaggaa cgatttccaa aagatcctgg
    21061 ccttcttcct gagaacggcg cagcgcttct ttgatgtcca cgataccgat ctgttcacct
    21121 ttgggtccga tcagtcgcac tttgggtgtg cgaatctgct cgttgatacg aattttgtct
    21181 tccttaccga tggctggtct ccttacttag taaacgtaat tagtcgagta ttttgtcttc
    21241 gatctgttgt ttgacctgcg ctataaattc cgccaatccg gcagcaccct gatcgccttt
    21301 gccatgacgg cgcaccgcaa cggtttgtgt ttgcatttct ttttcaccga caacaaggat
    21361 gtatggtatt tttttcatct ccgcttcgcg gattttataa ccgattttct cgttacgcgt
    21421 atctaattgg caacggattt tttctttttc caaagcgatt ttgacctgtt ccgcatagga
    21481 cgtatgcgca tcggaaatag gtagcacagc aatctgaacc ggtgcgatcc atacgggaaa
    21541 attaccggca aaatgttcga tcagaattga aataaatcgc tccatcgaac caaacggtgc
    21601 gcggtgtata attacggggc gatgtttttg cccgtctccg ccgataaact caagtccaaa
    21661 ccgctccggc atcacataat ccacttgcac ggtgccaagt tgccattggc gcccgagcgc
    21721 atcccgcacg ataaaatcaa atttaggccc atagaatgct gcatcgccga taccgacaaa
    21781 ataatctaat ttcatggcgt cggctacttc atgtatttcg cgctcggctt gatcccacat
    21841 ttcaggggat cccgcatatt tatccggagt tttgggatcg cggaaagaca gacgcgtttt
    21901 gacgggcatg ccaaatgtct taaacacaag ttgcgttaaa tcaatcgcgt tgatcaattc
    21961 atttttaagc tgatcatgcg tgcaataaat atgcgcatca tcttgcgtaa atccgcgtac
    22021 acgcgaaagt ccgcttaatt caccggactg ctcataccga tacaccgtgc caaattcagc
    22081 caatcgcaac ggaagatcgc gataacttct cggcttggaa gcatagatct gatgatgatg
    22141 cggacaattc atcggtttaa gaatatattc ttcgtcttcc actttgatcg gtgaaaactg
    22201 agaatccgca taatagggat aatgcccgga cgtacgatac aaattaagat ttccgatatg
    22261 cggtgttacc acttcctgat atccgcgtcg aagcaattcg tctttgagaa aattttccaa
    22321 cgtcctgcgc agcaaagtgc cattggggag ccaaaccggc aaccctccgc cgatcatcgg
    22381 cgtaatcata aaaagctcaa gttcgcggcc cagtttgcgg tgatcgcgtt ttttggcctc
    22441 ctcaagcatc gtaagatatt catcaagctg cttcttggac ggaaagctaa tagcacgtaa
    22501 ccgctgaagt tgaacattat tttcatcacc tttataaaag gcgccggcta ccgacatgat
    22561 cttaaaggat ttgatcatcc ctgtatgcgg gatatgcggg ccacggcaaa gatcagtaaa
    22621 ttcaccttgc gtgtaggaac tgatcggagc gccttccgga atacgttcga tgatatccag
    22681 tttaaaatcc tgaccgatct ttttgaaaaa ctccagcgct tcgcctcgtg tcagttctgc
    22741 acgtgtataa gtaagttttt ctttggcaat tttttccatc tccgcttcga tgcggacaaa
    22801 atcggcctca ctgatttttt ccggaatcaa aatatcatag aaaaaaccgg cttctgtcgg
    22861 gggaccgtct tcaaatttgg cctccggcca tagtcgtttg acggcttggg ccatgacgtg
    22921 cgccgacgaa tgccagaaaa cctgcatgcc ttcggcagta tccgaggtaa aaagtttgag
    22981 cgaagcatcg cccgtgatcg gagtggacaa atccacctgt ttgccattca cttcggcaac
    23041 gacacatttt tccgctaacc cgcgcgaaat ggacgtcgcg acagccatcg gggtaatgcc
    23101 ggattcaaag gaacgaacac tgttgtctgg aaacgtgata ttaatttgtg ccatatttta
    23161 acatcgtgaa ccgggattat tttcaacccg tccgctgagt gatgccgatc gggttatccg
    23221 gtagttctga tttttagatc agaacgtttt gtattgctgt actaaagaca gcattaaagt
    23281 gcgccaatag tagttgaaaa tatactgatt gtcaataaga attttagagt atgagcttat
    23341 tcaataagta agctgacgtt atttcataca atgcacgctc tcttatccgt tactttaata
    23401 caaatcagct aaattaattc attcttagca cagctttgcg cgcgctgtct tttgcggcat
    23461 cggataggtt aaaattataa ttaaacatag cgccgtccca atcaccgtat aacggattag
    23521 gtagaactat aaaacggtgc ccccattcat tcttcatttg atcaacacgc tggttgcgtt
    23581 cgggattcag acggccatca taggcatcgg ataaatcatt caggttatcg ccgcacaaca
    23641 caagaataga atgtgtttca ctgactttac gacggcgcgc ttctttattg gactccgctt
    23701 cgcgaaacat gatatgcaac gtgtccgctt gcggaaatcc gagcgaacgc aaattattca
    23761 aagtacccgg cgcttcagac gggcgacggt tggtgatata aaaaacatcc gcacccttgg
    23821 aaacggcata atttaaaaac tccttagcac cggggacagc catggccttt gcttcatccg
    23881 tccacgactg ccatcctacc ggatattgtt cgccgttggc tatttggcgt gcttgatatg
    23941 ggctgttatc taaaactgtc tcatcaatat ccacgacgac cgccggtttt ttagccggct
    24001 ttttggataa ctcgacatca agtgtcatac gtgcaacatt aaaagcctga taacacaagg
    24061 cacggtattc accggatatc tgcatccagt ttaatgcggc catcgtgtac tcatttcgcg
    24121 agaccgttgt ttttttatta ttgtcaacct ttggactaca cgctaatcca aaaaaaacca
    24181 cacccatgat aaaaaaatta tgctgtttca tgctcatcct tactgtagct ggttatagta
    24241 taatttataa tgttattttc tgaagccgtt ccgggatctc aacatggcga aatccgttac
    24301 tttttaatga ttccgcaaat ggcacgcttt gcgcttcttc gccgtgtacc aaaaaaacgg
    24361 aagcttgttt gccgaccaat tcggtaaaat taagcaaatc ggtgcggtcc gcatgaccgc
    24421 tgaacgaatt gattttatga acgcgcgctt ttacggaaaa cgtatcgcca aatattttta
    24481 ccgtctcggc tttgtctacc aatttacggc ccagtgtatg ctctgccata taacctacga
    24541 tgagcacggt gttattggaa ttctcaatat tatttttcaa atgatgtaaa atgcgcccgg
    24601 cttcgcacat accggatgct gaaatgataa tgcatggatc gtttatgtcg ttgagcttca
    24661 tcgaatcttt tacttcacga atatagcgca atccttcaaa cgaaaaggga ttatctccgc
    24721 tttgcaggag attacgcgca tctttatcgt aataatcagg gtatttgcga aatacctccg
    24781 taacattcac cgcaagcgga ctatccacaa atacgggtat ctcagggaca attttgtttt
    24841 catacagcaa attgagatga taaacgatct cttgtgtacg ctccacacta aaggccggaa
    24901 tgataatctt gcctttacgc gcaacggcat cctggatcac ttgtacaaat tcgccgtgta
    24961 tttctttcac cggatcgtga actttgttgc cgtaggtact ttcacaaatg agataatcca
    25021 acgctctcaa ttgatgcgga tcttcgatga tcggcagatt tttccgacct aagtcaccgg
    25081 taaaaccaaa tcgaaccttg cgcccgttgt gagtgatctc cagcaggatc atcgccgaac
    25141 caaacatgtg ccctgcgtta tacagcgtag cacgcacgcc cggcagcggc tcaaacgcct
    25201 gaccataacc aatccctaaa aaatgacgca tcgcctgctg cgcatcttcg atatcgtata
    25261 tcggctcaat cggaggctcg tctttttttt cacgcttata gttgacatgc tccgcatcgc
    25321 gcatttgtat aaaagccgaa tcctgaagca tcaccgaaca taaatccacc gtcggttccg
    25381 aacaataaat cgtaccttta aaaccttgtt tgacgaggtt cggaatattg ccactgtggt
    25441 caatatgcgc atgcgagaga atcatcgcat caacggaagc cggatcaaac gggaacgtac
    25501 gattccgttc aaaagcttcc tgtcgccgcc cctggaataa gccgcaatcc aataacagtt
    25561 tttgaccgtt gatacggatc agatgttgcg aaccggtcac actgcgggct gccccgcaaa
    25621 attgtatatc catgatactt ccgtaataat ttaattatac cgcccggcgt cgccgttcaa
    25681 tcaatacatc aaaaatagtc tgtataacat ccgattcgta tatcgtttta ccggcacgct
    25741 catgtttagc catttcgatc atcgcctctg cgaccgcctg ggcacgtatt gcgcggtatt
    25801 ttttccatcg gccgaccaat aaaaagccaa gcgttcgaaa cacaacgatt cccaacgctt
    25861 ctccgatgcg tcgctcggat ctttcgccga ccaaaaagga aggcctgaaa attgacaacg
    25921 aacgaaacct caacgtttcc aacgcgttct ccagctcgcc tttaacccgg ctgtaaaaaa
    25981 caccggaaga aggttttgca ccgatagccg taacgaccaa aaaattatta acgccgtttt
    26041 ctttcgctat acgtgcacat tcgaagggat atgtaaaatc cactttgtaa aaagcctgtt
    26101 ttgaaccggc cgtgcgcatc gtcgtaccga gcgtacaaaa cacatcatgc gctttgatcc
    26161 aatgcgcggc attggtcaac ttatcaaatt ccacgacgtg ttgcgacagt tttgcgtgcg
    26221 taaaagcaat aggcttacgc accaaaacaa tgacgcgatc atagtcccga tcttctaaaa
    26281 gcgcattgag cagatgctgt ccgataagac cggaagcgcc tacgatcaat gcggaacgtt
    26341 tattttgcat agtgctaaaa agatgataaa ccgtggttta gtttaaacta atttttgact
    26401 aaaaacagta gaaaaattac aacatttctt ttacattgat taatcataat ttctaaaatg
    26461 aagaggatgg taaaatgaaa acctggatat tatttttagc ggccgcattc tccttaaatg
    26521 cacagcaaat ttcgacaccg aggccaagcc cccgcgcaac cgtttcgcaa accgtcggtg
    26581 ttacggaaat gaccgtaagc tatcatcgtc cgggtgtaaa gggtcgtacg atttggggtg
    26641 aactggtgcc ttacggtcag gtgtggcgtg ccggggccaa cgaaagcacc accatcagtt
    26701 tttctgacga tgtcaccatc gaaggtcaga aaatacccgc cggtttgtat ggcattcaga
    26761 tgatacctga ggccaatgag tggatcgtta ttttgaccaa agacgccaaa ctttgggggt
    26821 catttaacta taaacccgaa aacgatgccg tgcgcatcaa agtcaaaccc acttccgcag
    26881 aaatgatcga acgtcttcga tattcatttg aagatgtcaa cgacaatagc accgtgctga
    26941 ctatgcaatg ggaaaaattg aaaatcgcat ttaaaataga aactgacaca ccgaatctga
    27001 cctttaccaa agcacgggcg gccatgaatt ggagcggact tatgcaggcc gctgcatatt
    27061 gccttgataa taataccaac ctcgacgaag gcatgaggtg gattgatctg tccatcggta
    27121 tgaatgaaaa ttattataat ctccgcacca aagcccagtt tgtagataaa accggaaaac
    27181 gtgccgacgc tatcaaagtc atggaacgtg ccatggagct ttctaaaaaa atgccctctc
    27241 cgccctttga tatcaagcgc atggaagaag tgctcaacga gtggaaaaag aaaaaataat
    27301 ttccggagtt ttcatgaaaa aaaatttttt ttacgtgttc atcgctatcg cggggatcat
    27361 cacggcacac gcccagcagc gcctcaaaac gcccgatgcc agtccgcgtg ccgtagtcga
    27421 acaaaccgtc ggtatgaccg aaatacgtgt aacctactac cggccggcag tgcatgaacg
    27481 aatcatctgg ggcgatctcg tgccttatgg cgaagtctgg cgcgccggag ccaatgaaaa
    27541 tacgaccgtt tcgttttcga cccccgtaat catcggcgat agagaaatac ctcccggcac
    27601 ttacggcctt catatgatcc cgatgcgcga caagtggacg atcatactca accgcgatca
    27661 caaggcatgg ggcagttttt tttacaatga gaaccacgac gttattcggt ttaatgtcga
    27721 accggaaaac tcggcatttc aggaacgcct gctttatact ttcgacggaa tcgaatcgga
    27781 ccgtgtaacc ctcgcgctca aatgggaaaa aatccgaata ccgattccga tccgcgttga
    27841 tctgcatgcc accgtcctgg ccggtttcag agacgaatta accaacgtgt ccggtttttt
    27901 ccctgaacct tatgttcagg ccgtcaacta ttgcgtacgg aataaaataa attacgatga
    27961 ggcgcttcgt tggattgatc actcgattga gctgcaggaa tcatttgcca atctgaatgc
    28021 aaaatcgcgc ctgctggcag caatgggaaa aaacaaggaa gccgattcgc tcaatgcgcg
    28081 ggccatgtat ctcgccacgg aggttgatct caacaatctg ggctatagcc tcatgggcga
    28141 aaataacttc aaagaggcct tggcgatttt tcagaaaaac gccaaagagc atccgacatc
    28201 atggaatgtt tttgacagcc tgggggagtt atacgaacgc atgaacgata aaaaagccgc
    28261 gatcgaaaac tatcgtaagg cgttatcact ggtaaaagac gggcggaata agcagcggtt
    28321 atctgaaact atcaaaaagt taagcggtcg ctgatcgcag gtaaatcata tcaaagttgt
    28381 ttcaagggta gttattaaaa gccatggtaa aacaaaaagg ttactatcct tggaacaacg
    28441 ttgttttttg tctgaagaaa taaatcaccc aggccaaacc caccaccaaa gcacatccga
    28501 tcacattgta ccacaaaaaa gaaatgggtg taaaaaagta acataaaaag acaataactt
    28561 cggctatgat cgccgccgta aaagccgatg tgccacccac ttttttccag taaaacgcca
    28621 ataaaaatat tcccaggatc gtgccataaa ataaggatcc cagaatattg acggcttcaa
    28681 tcagagaacc taactgaccc gccaattcgg caaatccaac agcgaatgca ccccaaaata
    28741 cagtagccca tttgctaacg cgtacgtaat gcgcatccga agcatctgag cgaaacatcc
    28801 gccggtaaat atccaccact gtagtcgagg ccaatgcgtt gagttctgcc gacgtggaag
    28861 acatggatgc cgcaaaaata gccgcgatga taagccctac caatccgacc ggaagataat
    28921 cgagcacaaa cgtgagaaaa atataattag tatcgttggt attcgccgaa ggatcatggg
    28981 ctttgatcag gtccttagcc ttttctttca ctgcggccat ctgtttgttg atttcgcgca
    29041 aactcgtctc ttgctcttcc atacgcattt catcaccgga ttcttttgcg gcattccaca
    29101 catgcagaat ctcttgttgc cgtttaaata atcccgtgtg atcgttttcc aatcggacat
    29161 aatcggcggc atacgaactt tgatatatac gttcggtttc caaacgattg aaaaaaacag
    29221 gcggcatggt aaattggtaa aatgcaaaaa ccataacacc gagtaaaaga atcaaaaact
    29281 gcataggtat tttcaaaaga ccattggtca aaagtccgag acgcgtctgt gcgatagact
    29341 gaccggtaag gtaacgaccg acctgtgatt gatctgttcc gaaataactt aactgtagaa
    29401 aaaaaccgcc gatgagccct gaccacaacg tataacgatt gtcccaatcc caggtaaaat
    29461 caaccgtatt cattttaccc tgacttccgg caaggtgcaa tgcatcgttg accgaaatgc
    29521 catccggcaa aagcgctatt accgtcacaa aagccgccgc catgccggtt aaaataatca
    29581 tcatttgatg aaattgtgtc cagttcaccg cacgtgcacc gcctgtcgtc gtataaatga
    29641 tcaccagtcc gccgatcagc agattggtca ataccagatt ccaacccaaa agtaatgaca
    29701 aaatgagcga cggcgcatat atcgtaagac cggcgccaag tccgcgctga atcaaaaata
    29761 gaaatgaagc cagcgccctc gttttgaggt caaattgttt ttccagaaat tcgtatgccg
    29821 tatacacttg taagcgccga taaaaaggta cggccgtgat acaaagcaac accatcgcaa
    29881 gcggaagacc aaaataaaat tgtacgaaac gcataccgtc ggcataaccc tgtcccggcg
    29941 tcgaaataaa agtgatcgca ctcgcctgcg tcgccatgat cgaaatggca accgtccacc
    30001 accgcatgtc gcgattggaa agaacatacg aatccattgt ggccacaccg cggcttttat
    30061 agataccgta cgcaataata aaaataagag aaacggcgag taccgtccaa tcgagggcgg
    30121 tcattggtag gcaatccgta tgatatagaa gatgaaaata taaacgacca acatcattac
    30181 ggtcaatccg taccaaccgc gccatgattg aaacggcgac ggtacgtttt gttcttcgtc
    30241 agacatacaa cccggattta aaatgtggat aaaacttgga tagtggtacg agtattaaga
    30301 aagatggttt cgtgtcacat caaaaagtcg agctctttta caaaatcatc cgtcagattt
    30361 tgaaccggcg gtgacgcctg tacgggatcc tgcatcactt cctgcaactc accgcgtagc
    30421 gcaatatcaa tgaattccgg gagcagctct tcatcaaaat gtttaccaat cccctcctca
    30481 agcaaatggc aagcctgaat ggcggtcaaa ggttcgcggt acgggcgcgc actggtcagc
    30541 gcgtcaaagg tatcggcaat agcaacgata cgcgcttcga tgggaatatc tttccctccg
    30601 attttgtacg gataaccttt gccatccatg cgctcatgat gataagctat gatattgatc
    30661 agcggttggc tggacttcag aggaccgcaa atttttactc cgatatccgg atgttttttt
    30721 atttcatcat attcttcagc ggtcaacttt ccttttttga gaagtaccga atcacgtacg
    30781 ccgattttac cgatgtcatg cagtatggcg cctttgtaca aaatatcctg aaaccctttg
    30841 ggtacgccga gcgcaacacc cagcttgatc gaatattttc cgactctttc ggagtgaccg
    30901 cgcgtatacg gatcgcgcgc ttctacagcc cgtgaaaatg cgagaatcac ttcatcaaaa
    30961 tcttcgagat ttgcatttaa ctctttcatg cggatggccg aacgaacgcg cgtcttaagc
    31021 tccatcgc
//
LOCUS       DAOQGF010000030        30987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_660_length_30987_cov_7.005949, whole genome shotgun sequence.
ACCESSION   DAOQGF010000030 DAOQGF010000000
VERSION     DAOQGF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_660_length_30987_cov_7.005949"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..2693)
                     /locus_tag="PK937_06260"
     CDS             complement(123..2693)
                     /locus_tag="PK937_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH32207.1"
                     /translation="MLMQTRFLKPRLKNYAMGVWCMMAGWCAPALVQTQSLKSVAVIR
                     FDNRSPLPENNWLGIGFAETLTSKLGMVDGLMVMERTKLHEILTAAGKTEPIDENRTE
                     VGLAYAKMIGVEYLLIGSVQVTGSYKNPASQLRINARMIKTSTADISGNLTFSANGNI
                     KDIFAIQTELAEKFCTALGVTSGLAGLTFEDAQSIEAQQWYGRGLKAYYEANYAAAIE
                     LFRSAVNNNEGVFYAAAHTMEGYARQKQIERAQDAEKQTYKQAYLDQFRRDAGEAAPA
                     FYDLGIAHQATGLYDDAVKSFGEYLRYMDKASQLVTECAPEVENPRRIQTVEPDSNEP
                     YDIFRPAVQKGFYYRLSKGSNYETNGVECVWNNGAVRWRSKLPDCGGESPQLNKMDLF
                     GDTLYMGAYVIAMVNNKTGKAYWQFPERPFLNDIGWPLLIEDVAFYKDKMYISANNTI
                     YIINRSTGKKLAEYRGGSGTYVKEGIFNIGANCVNVDRLLEQKLYTEVDALHQISKTY
                     LLAQRLDSALKYAEAVMQSYPNHIGANEITAHVALAQGQRKTAVDRIIQLYTLTPNHT
                     LRDIARSEFGILGVTTFHGWLATLKCDRDALWAVFGGEFNRGANVNELWRIDAVTDQT
                     VWRKAMDYEGQYTLSYDADSSYIYCAWPRKAWENNAETWKYDLLCLSKKNGQVVWKTL
                     LRDQCQVTEGVYSSSIVPRIDGDFLYITFYNGNVYKVSRSNGAIVWGRSCFGDGTIRR
                     YEANEEYFTRCNLGIVSQVVYVHVPMPSVQRDTVIGFDKITGQRRLVLPEMREFRAAM
                     PEDTTATVLFSGYGSYGYYPESVFTEKKIYAGSKDQGILILDRVILEKLRTDKKVWWQ
                     "
     gene            complement(2971..6174)
                     /locus_tag="PK937_06265"
     CDS             complement(2971..6174)
                     /locus_tag="PK937_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH32208.1"
                     /translation="MRIWDFAVDNKVTVYILMMIIVILGTMSYSSLPREAAPDISIPL
                     VIVSVPYVGVSPADMEGLVTQPMEKELKSLKDVKEITSSSKEGLSTIRVEFETGIDID
                     EALRRVRDKVNQTKPKLPSDILEPIISEINFSEFPIMFVTLGGDLGLARMKRIAEDLQ
                     DKIEAVPGVLSSDITGSLEPEVQVNCDINRLKGYSVSFDDVINAIRSENLTTPGGAIN
                     NGTTEYSVRIPGEFAAPKPIEDLIIKMRHGSPIYVRDVATVEYLFEDRSTYSRLNGSP
                     VLTLSVKKRAGENLIQIADEVKTILSDEKANFPPGLHVRITNDASIDIKRSVKELENS
                     VVTGMVLVVLVLFMFFGMKNSFLISTSIPISMLIGFVILSFMGVTLNFVVLFALVLVL
                     GILVDDAIVVIENIYRHQHEYGKNPIQAAKDATREVSIPVATSTFTTISGFLPMLFWP
                     GVIGDFMYYLPLTLIIMMGASIFTAYVISPTQGAQFIDYHKEMKALNSSMQQKHWWTH
                     YNPFSRLYHYVDTHFFPRAQEKYVKTLQLTLRHKTMTVVLSGVLLFAMTFLFVAFSKG
                     VEFFPETQPNQVSVNIAMPSGTPLEMTNAVTAMVEERMKHVEGVKDMEFVVANVGTSE
                     DPFDFGGQGTPNKSQISINFYEKMKREKNTFETMESMREHAKGIAGAEIKVKGQENGP
                     PVGAPVSIDLSGDDFRMLAQLSEKIQSEIRSIPGLVDLKDDYDAGKPEIQVIVDREKA
                     ALLEMSTGQIAMAVRTAINGTEASKFRVGEDEYKITVRLREDQRQSPSEIENLNITFM
                     NNRGVLMSVPLASIATVVHSNGLSNVRRKDLRRVITISGDVQNRLANDVLHDVREKLK
                     TFKLPNGYRIEYTGENKEMDKAMAFLAKTLVITILLIFLVLVSEFNSVKVPMVIMVSV
                     PLSLIGVFLGLLLTGTPFGVIMTGIGVISLAGIVVKNAIVLLDFTKHLRGSGMTLDEA
                     LAEAGRTRLRPVLLTAATTILGILPLASGIDFDWREWHFIIGAESSDFWRPLGVAVIF
                     GLTVSTFLTLVIVPTTYSWVEGITKAVGRWFKRETTV"
     gene            complement(6202..7251)
                     /locus_tag="PK937_06270"
     CDS             complement(6202..7251)
                     /locus_tag="PK937_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH32209.1"
                     /translation="MKIHQRLSLLLLPGYLWVTGCGSSNAGPGDTKKEPEAIVYIKTE
                     KIIAKPFAETIRLTGTVESVDNIIVPAEEGGRILRWLATKGATVQRGQAIAQIDDAAY
                     RSAYDAAQAAYQMAEVNYQKQKSAFAEQAISELQLKNLEFQRDAAKAQADLAKQRLDK
                     TTVTSPVNGVLNEQFADAGEMAAPGMPIAQIIDMTNLKVMVGVPERYARDVQPNLSVE
                     FTADAFPGETFRGKIGFVGAAVNPDNRTIPVELFLTNPGGKLKPQMIASVAMKLAAIE
                     KAILIQRDFVQQVDAGRLVVYVHKDGIAEERAVRIGGMSRDFVHILSGLQEGDEVITV
                     GYQNLTPQQAVRIQP"
     gene            complement(7274..8596)
                     /locus_tag="PK937_06275"
     CDS             complement(7274..8596)
                     /locus_tag="PK937_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH32210.1"
                     /translation="MRRCWFLLCICTGGWLAAQDSLLTLEEAIRIAEKNNALLAVARW
                     DMRKADQQVREAYGYAMPSITLDTRYTRALKKPVFFLPDFENPASGKVTPIEIGSNHS
                     IETGFTATQALFNFAVFTGVGTAKVYQRTSRALYRSEYNRMVASVKKVFYQVLLARQV
                     YETTKASLINAENNFNNVRILNKQGIVSDYDMIRAEVQVENLRPSVISAEQQALVVTN
                     ALKVQLGMDATDPLHIRGELTPVPIDSSWLDEQRAVTQNAGLEALRLQQDLNAAVSSI
                     RLAEYMPTLAAFGTYQWQAQKNTMDFGGKDFISTSQVGFTLSLNLFNGFQTTARLAQS
                     RADERKAEQQLRYTRENIRSQMQSIRLRLDEARKRMASQNRTVELAEKSYKIATTRYA
                     TGSGTQLEVNDADVALLQARLNRIHAIYDYLVTCVDLEEMISYHQPQN"
     gene            complement(8810..12226)
                     /locus_tag="PK937_06280"
     CDS             complement(8810..12226)
                     /locus_tag="PK937_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH32211.1"
                     /translation="MKVLSSVDSPNPILCNYKFLGSAMHHTRYLLLKVMLFLLFTSFA
                     NAQHNVRIVIPSHHTLGVTCFWMTEDGRYAVTGSKDNTLKLWDIHRGREIYSRKGFTN
                     FNSDGVLNFPMSVHISSDQRLAAWGDNYGNVSIWDLDSDSIVHVIMLGRSQMGGNLSI
                     RGLQFGSDNRTVLVIAEYAVKMYDAMSGAEIGIFCRSEEKITRIAVHGQTVLTGDRKG
                     TVRMWDFSTQSEIKVLRDGNNQVLGQITDICISNDGLLAAAGTYDKKLFVFDLNEKKT
                     KSEINIHKKHITALCFAERNQLMSASYDGLIFKTDIVNGITTPLTDKDLTSKLGAPTV
                     LGYSANKKILLCGTEWNTSMVLLHMDGDSSTAMLGRKTEPAMAIFTDPDEERIIVGYK
                     SFVSVWSLTYGKPQYNYAISPVRHYAFNPNRRLLAAAIGDTIHILNIVSNSVINQFRT
                     SDEDKTDFINALRFSHDGQTLAVGGLTGYDRPNLKIFDLTNPNKYFGANMDTHSVHQI
                     QFFKDGSLMIGGYNKCPIWWNWQTKKIKKLYAGPGSINYSIALSDDEKTAFFGFYIKN
                     YLMDVNTGRFRQSFVMNGTVRSALLPSDNKSVLGACYNESDPLEGIMEWDLDSGKKIG
                     AYRGHFGQVNQLNFINKEQFFLSAGNDGSVRLWNRQSKKEIAKFIGFDQGNGWVTVVD
                     NGYYMATKNITDHIYYSSGKKIYNFDQFDLQYNRPDIVLERLGKAPPELIRTYKNAYL
                     KRLQKMGFDPVNFESERSFNVPEITIPDLKTDLIETQNDTYTFSVHLKDKLYALDRLF
                     VEVNGVPLYGVRGKSIKAENMLNSHQTIKVTLSEGRNIIRASVLNTRGVESVSASLEV
                     YYRPHVPTKPVLYVVAIGVSQFIDSNMNLTYAAKDAEDMASLFSVNKDAYADVVIQTL
                     TNSQATRNNIVKTKNLLMKSTVNDEVLLFVASHGLLDDQLNYYIATHDVDFNRPSFNG
                     LSYDELESLTEGIAARKKLMLIDACHSGEVDKEETVLTNVAVHQESKGVSSRGFKTLQ
                     NKNSVGLINSFELMKALFADLRRGTGTIVISSAAGKEYAFESEAWKNGVFTYSLIEGL
                     KSKNADTNQDRSITVSELRDYVTRRVSDLTQGKQNPTSRSENLESDFKIW"
     gene            complement(12491..13375)
                     /locus_tag="PK937_06285"
     CDS             complement(12491..13375)
                     /locus_tag="PK937_06285"
                     /inference="COORDINATES: protein motif:HMM:NF023329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HNH32212.1"
                     /translation="MRFNRFSLLLVCAALFMWLHVAVNGYAQGAKGGSNAVKKTVTGM
                     TPSTESLTKWVPFTGVIPENAVVGGYENGQPMYVGRVAFEGGMHPGKVIGGGYCNIGW
                     GGKEYSYDKDFEILTAPANAVTWVAYKGTTPANAILGGYNDAAKKEPLYIAKHAYKDG
                     EHCGKLWANACNIGWGGAEVVLKNSIFVLAAKEEVKKPTGPYVRTENSHYLIGQIIPV
                     EYGNFPGSPSVWITVVPKGKKDTERGEYVYAIEKDGVVEINSVTLGEGEYELRAYFGA
                     FDTAAKARATIIMQGGGQ"
     gene            13869..14987
                     /locus_tag="PK937_06290"
     CDS             13869..14987
                     /locus_tag="PK937_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNH32213.1"
                     /translation="MKFSKLLPLVMAFITISFSISYGQTWQPAGGGSKKASVSELIGL
                     TNVTVEYYRPAVNGREGKIWGNIVHYGFADLGFGTTKAAPWRAGANDNTTIEFTTPVF
                     IEGKPLPAGKYGFFIAMSETKATLIFSKYNTAWGSFYYDPKDDALRVDVPVIKTAESV
                     ERLKYEFSDQTDDSAVLSLLWEKVKIPFKVSIDAKKEQIEVFRKAFNSAKFYVYWQNM
                     HAAANYCLVNNINLEEGMGWSERSINDFFGEANFSTLSTYAGFLEKFNRKKEADSVMQ
                     KAFPLGTALQIYSYARSLTKMKENQKAYEVFKIGYDKYPNDPNIILGMARGNAAIGNI
                     NEALNFCEKALTLFADPSSTGIIKKMMEDLKAGKDINK"
     gene            complement(15095..15706)
                     /locus_tag="PK937_06295"
     CDS             complement(15095..15706)
                     /locus_tag="PK937_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32214.1"
                     /translation="MTIVEIMLYATAFVLVLMALGGGLLIAGMSRSLGKLKERVRALE
                     HIQEERAAKKGAGTDDTTKIKSFETQIKALKAELEYLRSMVVAPTPAEPAASDKEVFY
                     VTASTTDGFIRKKDLKVSPGFDSLFKVELNADKQTGTVTIWTGSENMTQAITFHDRYL
                     QPLCEYTYNPQRSTRNVTVKKPGMIKLESDRYVIVEKMVIEFE"
     gene            complement(15836..19225)
                     /locus_tag="PK937_06300"
     CDS             complement(15836..19225)
                     /locus_tag="PK937_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32215.1"
                     /translation="MGYLLNLKPARSVAQDHWTDTTVFHPNMINSIEDNQSDKVERPI
                     ASIPSPFAQMHLFDYAFRSVNGRGEMSGNSLYHRLISYTFDVMELLFHANNIGGVGGK
                     LEVKEWVFNHQIDGLITSRSDGHRILGETLKLFIEQDRDSANFGSFDRFYLIAYNHRV
                     FAATSPLTMFFTVPHHATLKTIDIRRTNGTRYFETTLALHERDRDFQMFVHRLIAGHR
                     EFSGIYRSVHDYVDKNRPWIKDRELRIAVDAIYANGFSEHDFRSEYTEPKNLKLYLHQ
                     VGWRCARDLTNRISERSGFLMDVTKPTDGLIPMVLSKQFTDPGVIYVDADWNEEVRRQ
                     VPVTDPNPLESRTLPGTQIPYPYLLASDLLENSLIKLPYKAHPSFLTLTHSDEDESYM
                     LPVKLFYYNYFSPEDLRKQISFTVDGDAVTVRLQIPTRSGKRVELKRRYVPDPIEGTD
                     TGRIVEWKFDVAVFPFYRVKQEAESVERYNVMMVDGQRNRKDFPDLTFFDSRMEPLSE
                     SKSKQSPVKKIVRHDKKVFDGGVYYQITGAQFDYIRCELKGPQKAVGWIVPMWRYVEP
                     GTRAYTFAVDFGTTSTHIAYADATGAPKPFDFTKEDMPVVNLYGPEKISNRDAVTDLY
                     RRYNDRTVLPQSSVYQENDFVPTFIGAAGSPIQFPIRTATSESPGFVREETFTLGNIN
                     MAFNHERHVEFDDEIVHTNIKWKINLDEKTTERIRAFFEEILLLIRHKVVLNQGDPAK
                     TKLIWFHPLSMSAHAIGLLKSDWTQLAQKVLGISEQAVIEMTESQAPYYYHKATTAVV
                     GDSNVLCVDIGGGSTDIVVFEKRQPKFGSSFNFGCNVLWSEGNVLASRVNNKDNGIFR
                     EAYQAVFRNAPPNNKEWNQLRDICEQYLANERKRSEDVMQVFFKHEHLTQISERLGKD
                     QKFKIIFLAYYAATVYHALQCMRTKKLQAPHYICFSGNGSRVLGFIDRDPALKNLTAF
                     TRILAEAVYGNGAKEMRLVVSKEPKQATCIGGIVRQKENEQPIEAMIHLGGKKKNDAK
                     GLTYDAVSDGVKKDVQDNIKDFINLFFDLNEQISFHEYFGIEADLAMCRTELQKDIIH
                     LISDGISHQVLLDGTMENHPVKETLFFYPLVHAFYNLSKVL"
     gene            complement(19250..20662)
                     /locus_tag="PK937_06305"
     CDS             complement(19250..20662)
                     /locus_tag="PK937_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32216.1"
                     /translation="MSKLYIFGIGGTGARVIRSLTMLLASGVRIEADEVVPIIVDVDL
                     TNGNTVQSEETLKWYQKIRSRVQPESEFFTTPMRTIGQITGADPTAMFRFSFDSTHQK
                     KFRDFIDYSKLSAEDQFMVNLLYSEEYLDDELTEGFKGKPNMGSVALNTLTSMDEYDQ
                     FVNSFQPGDRIFVVSSIFGGTGAAGFPILLKNFCSQKSDPKLQKALKGALTVMPYFGV
                     QADNESQIDSNTFHAKTKAALAYYEKNLSGLHHLYYLADTIKELYPNFEGSTGQRNKA
                     HVIEVLGALAIIDFARQTFETSGAKTLCHEFALKEDGEVLTFTNLPDPAQALIKAPLI
                     RFLYLYYIMQFLLRSERKDAWIKNIFGTASALNDEDAKLLQKFLYMYADWITELAENK
                     RAFEPFYMQQFAESFAHALVKGYQLEPGGLFRTGLDDNFIISALGSNSRQLPATLTTM
                     GKLIKAGYAATDAVIQRFYK"
     gene            complement(20675..21061)
                     /locus_tag="PK937_06310"
     CDS             complement(20675..21061)
                     /locus_tag="PK937_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32217.1"
                     /translation="MSDMFTALYEVWHGRQLEAVWRREIYPLAGFLMLLYGVFSAWLY
                     YFFLSLRIPKTAVDTRFFWSYNGLMAFLFGLIALGVFKWNTTINGLPGSAMLFMLANM
                     AYAFILSLVAGLAMKRYSTNASTIPF"
     gene            complement(21065..22315)
                     /locus_tag="PK937_06315"
     CDS             complement(21065..22315)
                     /locus_tag="PK937_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32218.1"
                     /translation="MVPFTKTFHRLRVIGLTSLIVVSVVLFGCKSDKDGDRVEDAKDE
                     CDNARGVAVDANGCPCVDSATLPEPLPIAAVRMYVDNSGSMRDFTNPTSLFRSTISEC
                     ISRIQKMKTDNIADTVRYHLIASSLLHTDYNILYKAIRTGDIMKAKSTPILSMIEQVQ
                     RRHEAQDISVLVSDFELSEKDADNLDLALKTDVLAQIAKHNAVGLYAFREKSVDRPYY
                     VMVIGTSASVLLFDREGGLRDYRHKIFLGVKYPVPEIAFLNYSQRDETTWFISQDFCR
                     TIEDASLAGKGYIQFAMGLDLKKYEGLQLDSVYLTKNTLIKDVDPQHVAMRWYTAHQF
                     KNLADEKEAEQLSTYSHVVVVKLHRLFENTNKYFFELAKAQDFERFYGSDPSMQALVH
                     GFREAYRGDTGPYFSVKLNIVKEQ"
     gene            22704..24284
                     /locus_tag="PK937_06320"
     CDS             22704..24284
                     /locus_tag="PK937_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNH32219.1"
                     /translation="MSTNKEAWGSRVGLILAMAGNAVGLGNFLRFPVQAIQNGGGAFI
                     IPYLVCFLLMGLPLLFVEWAMGRYGGKNGHHSTPFILDSMDKRSFWKYIGVFGIFTNL
                     AVAAYYCYLESWTIAYVYHMLVGSFQGQNQDEVAGFFNHYLSFEGFENVIVWLLCLFL
                     NTWILSKGLSGGVEKVAKIGMPLLILFGILLAIRGFTLSAGENGAIYDGTVGLNFLWT
                     PQFDTIWDGKVWLAAAGQIFFTLSVGMGSIHCYASYVKSKDDIALNSMSAGWMNEFVE
                     VVLGSAILIPIAVGYLGIDKVIEMTASGGLGLGFKTLPYLFTQWGPILAALAGVFWFG
                     LLFFAGITSSLAMGTPCMGFMMDEFGWKREKAAWAFGFTILILGLPTVLFFHHGVFDE
                     YDYWAGTVSLVIFALFEIILFAWVFGMDKGWKEITDGADIKVPNVFKFIIKFITPILL
                     GWVFVKSLPDIWDTISNKKLYDKIAATTDTVQIAAIQESIMYVNASRFVLLGIFVGVS
                     ILVFIAYKKRIREGRFTS"
     gene            24281..24424
                     /locus_tag="PK937_06325"
     CDS             24281..24424
                     /locus_tag="PK937_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32220.1"
                     /translation="MNSSALAFMLITYIVVIGATGYFFYLALTTKPHDEPDSFTENDN
                     VPR"
     gene            24577..25449
                     /locus_tag="PK937_06330"
     CDS             24577..25449
                     /locus_tag="PK937_06330"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNH32221.1"
                     /translation="MHLGYLFKESFSGLRRATLSTFVAVTIIAIALSLFSFFLIITLN
                     INQLVEDIRSRVELEAFLDRALDTPQSKKIEAAIRDIEGVESVTFISKSDAARILKKA
                     MGADDIFDLVESNPLPASFKIRLQSEYRNWQNVLAVAQTLETIDGVEEVSYQKELLLA
                     LDEKVDLYNMISIGLGCIAALAAVLLVSNTIKLSIYAKRDVIKTMKLVGAKNSFIAAP
                     FILEGILQGVLGSLIATGIAYALIRIVQRYLLPTLQFSWEIPAIVVGFGILLGLFGSF
                     VSIRFFLKEKISDM"
     gene            25472..28408
                     /locus_tag="PK937_06335"
     CDS             25472..28408
                     /locus_tag="PK937_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH32222.1"
                     /translation="MPRCITSSLFIALILTTASLRAQEETGYPYIKNYTMRDIPAGLQ
                     NWAVAQDQRGIMYFGNSNGLLIFDGIHWRVTETPGRAFIRSIAINTSDEVFAGAVDDL
                     GYLWPDSNGQRQFVSLLSAIPEPDRHFGDVWKSFCIGNRIVFQTFSRIFIFESAPPES
                     PERFRFVRTIQPPNSLHFSFQVRNAIYMIVRGHGLHLLQNDTLVLAPGGAFFASDRIY
                     AMLPWENDATMIFTRERGIFLWKDGKIAPFAGDVHTDLPRHQIYHATLLSDSTVAIAT
                     LDNGIWIMNRFGRRIAAFDRSSGLMDQSILYLFTDRENNLWAGLNQGIARVEYPSRYT
                     FFSERNGLTGSVFKIARHQGNLYVATFDGLYRLSKNNTHQLERVTGISSSVWHLYSAG
                     TSLVIAATDGVYALRDHVIQRISDRPYNYVIAPTLSDAKQILVGHAKGVDIYRQQNGR
                     WISTAAWQGIDDEIRTITEYAPGKFWLGTMFRGVMHYTTPGQVTRYGASHGLPPGEIR
                     PFMINERLLFATSSGIMTFDKDRFLRPPALQAPDDVFVGYGVWMHAVDRHNFWVSVET
                     KRGANTYRATMDDNGSIHYGKPILVRADRHVIWNGMQDDDGIAWFGGPDGIIRYDAHT
                     ADNTTAPFHALISRITLQSDTSRAEYNIRFRNPTQIPEYPAMGIRFECAATSFTRQEF
                     NEFQYWLEGFNHMWSDWTTETRRDYTNLPEGVFTFRVRARNFAGQIGKEDAFTFRVTA
                     PWHKSNWFMSLTAMVLVGFLIGAVRFATVRRWKRRVMDLELRERISRDLHDEIGANLS
                     NIALLSEMLSAKEKFENKEKEQLARISNVARRTVDTMRDIIWAIQPHQDQLDEMMLRM
                     KDLTSELLGDTLPYTLDFPSENPTRTVTMPYRQNIILIYKELLHNIIKHAQASFVHIR
                     LVITGDELVLVVTDNGIGFDAQHSHDGGYGVKNLKRRTRGIRGDIRWETAENGGTRTI
                     LRCTLA"
     gene            28483..29046
                     /locus_tag="PK937_06340"
     CDS             28483..29046
                     /locus_tag="PK937_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNH32223.1"
                     /translation="MIIHRYLRQKPAIPKSVFLATGAIIVGDVVMGEDCSVWFNAVIR
                     GDVNTIRIGSRTNIQDGAIIHCTLDKYATVIGDDVSIGHGAIVHGCTVHDHVLIGMGA
                     KILDRAVIHPYAMIAAGAVVREGFEVPERTLMAGVPAKPVRSLTDDEIAMIDRIPQNY
                     IRYAADYRTLNVLDSAVVFSSSQTSNV"
     gene            29043..29414
                     /locus_tag="PK937_06345"
     CDS             29043..29414
                     /locus_tag="PK937_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32224.1"
                     /translation="MKKIVIIDDDQDFVSVAGKVLSHIGKFDVVAHTDSSDAMDLIRR
                     EKPDLIIVDIMMPKVDGFTILRNVRTSADLQHIKVIVCSAKIFDVDKKNALKLGANEY
                     ISKITAASELVDKVNAVLGTV"
     gene            29439..30317
                     /locus_tag="PK937_06350"
     CDS             29439..30317
                     /locus_tag="PK937_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH32225.1"
                     /translation="MKYKFWGVRGSIPTPGPETVGYGGNTSCGELTLDDGSMIIFDMG
                     SGIRVLGNDLMKRGKKPVEAIILLSHTHWDHIQGFPFFVPAFIPGNKILICGCEEADI
                     KLETIIKDQMKSAYFPVELSDMPAAIGFKRLYEGRFKISGARVDTMYLNHPGFTLAYR
                     VEYNGKSVVYASDNEPYPIQPNDSPEMAGMKYQGKDNHRIIEFSKNCDLLIHDCQYTP
                     EQYKTKVGWGHSPYDYVAEIAWKANVKRLAVHHHDPSHDDAFVDGIVNNVKNLLREKG
                     ARAEAFGAREGLEIEL"
     gene            30431..>30987
                     /locus_tag="PK937_06355"
     CDS             30431..>30987
                     /locus_tag="PK937_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32226.1"
                     /translation="MNIVELDNIVLYLDKQVLGKEPNISLKDLKSKDIPSFYKNYFDR
                     EAEKWIREESSMFLSSGRFNYDDPEVRAKFEELYMILKNKAIFNRSKLHRNMEQAVKL
                     QSNFLIAPQRTMVQFIYRDDETLTPAEVKDSMKYFLDYDYYYKALETYFATNAMETIS
                     RAKFKNFIAQLDEKALSQDKTVAAV"
BASE COUNT         8188 a         7514 c         6814 g         8471 t
ORIGIN      
        1 agcatggctt gtttttgtga aaaagccgaa aggagaggga taagtaaaag aaaaaaaact
       61 ctgcgccatt gttgattcat catcaagacc tatctgatat gacttagatt cattttttaa
      121 ttttactgcc accaaacttt tttatcggta cgaagttttt ctaaaataac acggtctaat
      181 atcagaatac cctgatcttt tgaacctgca tatatttttt tttcagtaaa gacggattcc
      241 ggataataac cataactacc atatccggaa aataaaaccg ttgccgtcgt atcctccggc
      301 atcgcggccc ggaattcacg catctcaggc aatacgagac gccgctgccc cgtgattttg
      361 tcaaatccga taaccgtatc tctttgcacg gatggcatcg gtacatggac gtacaccact
      421 tgggaaacaa taccgagatt gcatcgcgta aaatattcct cgttcgcttc gtaccgcctg
      481 attgtgccat cgccaaaaca actgcgtccc cagactatcg cgccattgga ccgagacacc
      541 ttatagacat tgccattgta aaacgtgatg taaagaaaat caccgtctat acgcgggacg
      601 atagaggacg aataaacacc ttcggtcacc tgacattgat ccctgagcag agttttccaa
      661 accacctgac cgtttttctt agacagacaa agaagatcgt atttccatgt ttctgcgtta
      721 ttttcccacg ctttgcgcgg ccaagcgcag tatatgtacg acgaatccgc gtcatacgat
      781 aaggtatatt gcccttcata gtccatcgct ttacgccata ctgtctggtc agtaaccgca
      841 tctattcgcc ataactcatt gacattggct ccgcggttaa actctccgcc gaatacagcc
      901 cacagagcat cgcgatcaca cttgagtgtg gctaaccatc catgaaacgt cgtgacgccc
      961 aggataccaa attccgaacg cgctatatca cgaagcgtat gattgggcgt caatgtatag
     1021 agctggatga ttcgatccac cgcagttttt ctttgcccct gtgccagcgc cacgtgcgcc
     1081 gtgatctcgt tggcaccgat gtggttggga tacgattgca tcacggcctc ggcatatttc
     1141 aatgcagagt ccaaacgttg tgctaagaga tatgttttgg aaatctgatg cagcgcatca
     1201 acttcagtat ataatttttg ctccagcaga cgatctacat taacgcaatt ggcaccgata
     1261 ttaaaaatgc cttctttcac ataggtgccg ctacctccac ggtactcggc caattttttt
     1321 ccggtagatc ggttgataat gtagatggta ttattggcgc tgatgtacat tttatcttta
     1381 taaaacgcga catcttcgat gagcaacggc cagccgatat cattgagaaa cggccgctcg
     1441 ggaaactgcc agtacgcttt tcctgtttta ttattgacca tagcgatgac gtacgcgccc
     1501 atgtatagcg tatcgccaaa caaatccatt ttatttaatt ggggactttc acctccacaa
     1561 tcaggtaatt tgcttcgcca ccgtacagcg ccgttattcc aaacacattc gacaccgttc
     1621 gtctcatagt ttgatccttt ggagaggcgg taataaaatc ctttttgaac ggcaggacga
     1681 aaaatatcgt agggctcatt gctatccggc tccaccgtct gaattcgtcg tggattttct
     1741 acttcgggtg cgcattctgt taccaattga ctggctttat ccatatagcg cagatattca
     1801 ccgaaagatt taaccgcgtc atcgtataat ccggtcgcct gatgtgcgat gcccagatcg
     1861 taaaaagccg gtgccgcttc acccgcatct cttcgaaact gatctaggta ggcctgcttg
     1921 tatgtttgct tttccgcatc ttgcgcacgc tcgatctgct tttgacgtgc ataaccctcc
     1981 atggtgtgcg cggcggcgta gaatacgcct tcattgttat tgacagcgct acgaaatagt
     2041 tcaatcgctg cagcatagtt tgcctcataa taggctttca gccctcggcc gtaccactgc
     2101 tgcgcttcga tgctctgagc atcttcaaat gtcagaccag ccaagcccga tgtcacaccc
     2161 aaagccgtgc aaaatttttc agccaattcg gtttgaatcg caaaaatatc tttgatgtta
     2221 ccattggccg aaaatgtcag attgccggat atatccgcag tggaagtttt gatcatgcgg
     2281 gcattgatcc gaagttgact tgcagggttt ttataactgc cggttacctg aaccgatccg
     2341 atcagcagat attctacgcc gatcatcttc gcatacgcta aaccgacttc tgtgcggttt
     2401 tcgtctatcg gctctgtttt tccggcggcc gtcagtattt cgtgaagttt tgttcgctcc
     2461 atcaccatca gaccatccac catacccagc ttagaggtca gtgtttcagc aaagccgatt
     2521 cctagccaat tattttccgg taatggagaa cggttatcaa aacgaatcac cgccacggat
     2581 ttgagggatt gcgtctgaac gagggcgggc gcacaccatc cggccatcat acaccataca
     2641 cccatggcat aattttttaa tcgcggtttt aaaaaacgcg tttgcataag catttcccgc
     2701 tccttcagga cgtgattact gtgggttaga attaaccccg ttctttaatg ggatttgata
     2761 tgtcagtgta tatatgaacc gatactcaaa aaataacaaa agtcaggttt agataaagtt
     2821 attttttttg gaaattcata gaatatcccg aatgaaaggt taattcatga ggcaaacggc
     2881 tccgatagaa tgccgcaaaa aaaatcggta ttcatgtttt ttgacatcaa taccgatgat
     2941 tttataatgc ctacttatat acgcaaatat ttacactgtc gtttctcttt taaaccagcg
     3001 accgacggct ttggtaatgc cctccaccca agaatacgtc gtcggaacga tcacgagcgt
     3061 aagaaacgta gaaacagtca gaccgaaaat gaccgccaca ccgagcggac gccagaaatc
     3121 gctgctctcc gcgccgatga tgaaatgcca ttcgcgccaa tcaaaatcaa tacccgatgc
     3181 caaaggtaaa atgccgagta tcgtcgtggc cgccgtgagc aatacaggtc ggagacgtgt
     3241 tcgaccggct tcggcaagcg cctcgtccag cgtcataccg ctgccgcgta aatgtttggt
     3301 aaaatccagc agcacaatgg cgttttttac cacgataccg gcaagcgaaa tcacaccgat
     3361 ccccgtcatg atcacgccga acggcgttcc ggtcagtaac aaaccgagaa acacaccgat
     3421 caatgaaagc ggcaccgata ccatgatcac catcggaacc ttcacgctgt taaactcgga
     3481 cacaagcacc aaaaaaatca gaagaatcgt tatcaccagc gtttttgcta aaaacgccat
     3541 cgccttatcc atttctttat tttcaccggt gtattcgatc cgatacccgt tgggcaattt
     3601 aaatgttttc aatttttcac gcacgtcatg aagcacatca ttggctaagc gattttgtac
     3661 atcgccggaa atcgtgatca cccgtcgcag atctttgcgg cgtacgttgg ataagccgtt
     3721 gctgtgcacg accgtagcga tcgaagccag cggcacggac ataagcacac cacggttatt
     3781 cataaacgtg atattgagat tttcaatttc cgagggcgac tggcgctgat cttcacgcag
     3841 acgaacggtg attttgtatt cgtcctcgcc gacgcgaaat ttagaggcct ccgtaccgtt
     3901 gatcgccgta cgcacggcca tcgcgatttg ccctgtgctc atctccagca gcgccgcttt
     3961 ttcacgatca acgatcacct ggatctccgg tttaccggcg tcataatcat ctttcaaatc
     4021 caccagcccg ggaatggagc gaatttcaga ctgtattttt tcagaaagct gcgccagcat
     4081 acgaaaatca tcaccggata aatcaatact caccggcgca cccaccggcg ggccgttttc
     4141 ctgacctttc actttgatct ccgcgccggc gattcctttg gcatgctcgc gcatggactc
     4201 catcgtctca aacgtatttt tttcacgttt cattttttca taaaaattaa tggaaatctg
     4261 cgatttattg ggcgtaccct gtcctccaaa gtcaaacgga tcttccgacg taccgacatt
     4321 ggcaaccaca aactccatat ccttcacgcc ttcgacatgc ttcatccgct cttcgaccat
     4381 cgctgtcacc gcattggtca tttccaaggg cgtacccgac ggcatggcta tattgacaga
     4441 aacctgattg ggctgtgttt cgggaaaaaa ttcaacacct ttgctaaacg ctacaaacag
     4501 aaacgtcata gcaaaaagca ataccccgga cagcacgacg gtcatggttt tatggcgcag
     4561 tgtcagttgc agcgtcttaa catatttttc ctgtgctctc ggaaaaaaat gcgtatccac
     4621 atagtggtag agacgactaa aagggttgta gtgcgtccac caatgctttt gctgcataga
     4681 agaattgagc gccttcattt ctttatgata atctatgaac tgcgcaccct gtgtcgggct
     4741 gatgacatac gcggtgaaaa tggaagcccc catcatgatg atgagcgtca acggcagata
     4801 atacataaaa tcaccgatta cacccggcca aaaaagcatc ggaagaaaac cggagatcgt
     4861 cgtaaacgtc gaagtagcca cgggaatcga cacttcccgt gtcgcatcct tggcggcctg
     4921 aatcggattt ttaccgtatt catgctgatg acggtatata ttttcgatga cgacgatggc
     4981 atcatccacc agaataccca aaaccaacac cagtgcaaac aaaacgacaa aattgagcgt
     5041 aacacccatg aagctgagaa taacaaatcc gataagcatc gaaatcggta ttgacgtgct
     5101 gatcagaaac gaatttttca ttccaaaaaa catgaagagc accagcacga ccagtaccat
     5161 ccccgtcact acggagtttt ctaattcctt cacggaacgt ttgatatcta tcgaagcatc
     5221 attggtgata cgtacatgca ggccgggcgg gaaattggct ttttcatcgg acaatatcgt
     5281 tttcacttca tcggctatct ggataagatt ttcacctgcg cgttttttga cgcttaacgt
     5341 aagcacaggt gatccgttca gacgactata ggttgatcgg tcttcaaaca aatattcgac
     5401 agtagccaca tcgcgcacat agatcgggga accgtgcctc atttttataa tcagatcttc
     5461 gatcggttta ggcgcagcaa attcgcccgg aatgcgcacg ctgtactccg tcgtcccgtt
     5521 attgatcgca ccgccgggcg tcgtaagatt ttcactgcga atggcattga tcacatcgtc
     5581 aaaactgacg ctgtagccct tgaggcgatt gatatcgcag ttgacttgca cctccggttc
     5641 cagactgcct gtaatgtccg aactcagcac tcccggcacc gcttcgattt tgtcttgcaa
     5701 atcctcggcg attcgcttca tacgcgccaa tccgagatcg ccgccgagcg tgacaaacat
     5761 gatcggaaat tcgctgaaat tgatttcact gatgatcggt tccagaatat ccgacggcag
     5821 cttgggtttg gtctgattga ctttgtcacg cacacgacgc aaggcttcgt ctatatctat
     5881 cccggtttca aattcgacac gtatcgttga cagcccttct ttggaagacg acgtgatttc
     5941 tttgacatcc ttcagcgatt tgagttcttt ctccatcggc tgtgtgacga gtccttccat
     6001 atccgccggg cttacgccga cgtaaggaac ggaaacgatc acaagaggaa tggatatatc
     6061 cggcgcggct tcccgcggaa gactgctgta tgacatcgta cccagaatca cgatgatcat
     6121 cataaggata tacaccgtga ctttattatc gactgcaaaa tcccaaattc tcatgggaaa
     6181 attccttgaa gctaagtttc gttatggctg aatgcgcacg gcctgttgcg gcgtcagatt
     6241 ttgatagccg accgtaatca cttcatcacc ctcctgcaaa ccggatagga tatgaacaaa
     6301 atcgcggctc ataccgccga tccgcacagc tcgttcttcc gcaataccgt ctttgtggac
     6361 atacacgacg agccggccgg catccacttg ttgtacgaag tcgcgctgaa tgagaatggc
     6421 tttttcgatc gcggccaatt tcatcgctac ggaagcaatc atctgcggtt tgagttttcc
     6481 gccgggattc gtgagaaaaa gttctaccgg aatcgtccgg ttatccggat tgacggccgc
     6541 gccaacaaat ccgattttgc cgcgaaacgt ttcacccgga aacgcatccg ccgtgaattc
     6601 cacgctgaga ttgggctgca cgtcacgcgc ataccgctca ggtacgccga ccatgacttt
     6661 gagattggtc atgtcaatga tctgggcaat cggcatgccc ggcgccgcca tttctcccgc
     6721 atcggcaaac tgttcattga gtacgccatt aaccgggctg gtaacggtag ttttgtcgag
     6781 acgttgtttt gccagatcag cttgtgcttt ggccgcgtcg cgctgaaatt cgaggttctt
     6841 aagttgcagt tcagatattg cttgctctgc aaacgcgctt ttctgttttt gataattgac
     6901 ctcggccatc tggtaagccg cttgcgctgc atcgtaggcg gaacggtaag cggcatcgtc
     6961 aatctgtgcg atcgcctgcc cgcgctgaac cgtagcgccc ttggtggcaa gccaccgcag
     7021 gatacgtccg ccttcttccg ccggtacgat aatattatct acggattcta cagttcccgt
     7081 aaggcgaatc gtctcggcaa agggtttggc gataattttt tctgtcttta tatagacgat
     7141 cgcttccggt tctttttttg tgtcgcccgg accggcattg gatgaaccgc agcccgtcac
     7201 ccataggtaa cccggtaaaa gcagaaggct taatctttga tgtatcttca taacagctcc
     7261 gtcgctggta aaattaattt tgcggttgat gataactgat catttcctca agatccacgc
     7321 aggtcacaag atagtcgtaa atggcgtgaa tacggtttag gcgtgcctgc aataaagcca
     7381 catcagcatc gttgacttcg agttgtgtac ccgatccggt ggcataacgc gtagtagcga
     7441 tcttgtagct tttttcagcc agctccaccg tacggttttg gctggccata cgtttacgcg
     7501 cttcatccaa acgcagacgg atgctttgca tttgactgcg aatgttttcg cgcgtgtagc
     7561 gcagttgctg ttcggctttg cgttcatccg cgcgggattg ggctaatcgc gccgttgtct
     7621 gaaaaccatt gaacagattg aggctcagcg tgaagccgac ttgcgatgta ctgataaaat
     7681 cttttcctcc aaagtccatc gtatttttct gcgcctgcca ttgatatgta ccaaacgccg
     7741 caagggtggg catgtattcg gccagtcgta tagacgaaac agcggcatta agatcttgtt
     7801 gcaatcgcag tgcttccaat ccggcatttt gtgtcaccgc acgttgttcg tccaaccaac
     7861 tgctgtcaat cggtacaggc gtcagttcac cacggatgtg cagaggatcc gtggcatcca
     7921 tcccgagctg aactttcaga gcattggtga caaccagcgc ttgttgttcc gctgaaatca
     7981 ccgacggccg caggttttct acttgcactt cggcacggat catatcgtag tcggatacaa
     8041 taccttgttt attgagaatg cggacgttgt taaaattatt ttcggcattg atcagactgg
     8101 ctttggtggt ttcatacacc tgcctcgcca gcagtacctg ataaaaaact tttttaaccg
     8161 aggccaccat acgattgtat tccgaacgat ataatgcccg cgacgtccgc tgatatactt
     8221 tggccgtgcc gacgcccgta aatacggcaa aattaaacaa ggcttgcgtt gcggtaaatc
     8281 cggtttcgat agagtggttg gaaccaatct cgatcggtgt cactttgccg ctggccggat
     8341 tttcaaaatc cggaagaaaa aaaaccggtt ttttgagagc acgggtatag cgtgtgtcca
     8401 gagttatgct gggcatggca tacccgtatg cttcgcgcac ctgctgatcc gctttgcgca
     8461 tatcccagcg cgcaacggca agtaaagcgt tgtttttttc ggcgatacga atcgcttcct
     8521 ccagcgtcaa cagcgaatcc tgcgccgcca gccatcctcc ggtacatata cataataaga
     8581 accaacatcg tcgcatgaaa caatctcctt tacttaaacc gtacagtcta tttcctctat
     8641 cggtccggca atcaactcca tctgtcttat cacaaagaat tgcaattcca tactttttca
     8701 atcacgtgta ccgtgcacat acatcgccct gatctaccca ctaaaaccat catcgcggtt
     8761 atcatgattt ttcgttgcac gtttttttgc tatttcactt tttacaaagt caccatattt
     8821 taaaatctga ttccaaattt tcagatcgcg aagtaggatt ttgtttgcct tgcgttagat
     8881 cagaaaccct gcgcgtcaca tagtcgcgaa gttcggaaac cgtgatcgat cgatcctgat
     8941 ttgtatcggc gtttttagat tttaaacctt caatcaaact atatgtaaat acgccgtttt
     9001 tccaagcctc tgactcaaac gcatattctt tacccgcggc ggatgagatc actatcgtac
     9061 ccgtgccacg tcgtagatcg gcaaataatg ccttcatcaa ttcaaaacta ttgatcaaac
     9121 cgaccgaatt tttattttga agtgttttga acccgcggga tgatacacct ttactttcct
     9181 ggtgcacggc gacatttgtc aatacggttt cttctttatc aacttcgccg ctatggcacg
     9241 catcaatcag cataagtttt ttgcgcgctg caatcccttc tgttagcgat tccagttcat
     9301 catacgataa accattgaaa gatggacgat taaaatcaac atcatgcgta gcgatgtaat
     9361 aattgagttg atcatccaat aaaccgtgcg acgcgacgaa aagcaagact tcatcgttta
     9421 cagtggattt cattaaaagg tttttagttt tcacaatatt attacgcgtg gcttgcgaat
     9481 tggtcaatgt ttgaataacc acatcggcat aggcatcttt attaaccgaa aacagtgacg
     9541 ccatatcttc tgcatccttg gcggcatacg tcagattcat attggagtca ataaactgcg
     9601 aaacgccgat ggccacaacg tacaaaacag gtttggtcgg aacgtgtgga cgatagtaaa
     9661 cttctaagga tgcgcttacg gactcaacgc ctcttgtgtt taaaaccgat gcacgaatga
     9721 tattacggcc ttccgataac gttaccttaa tcgtctgatg actgttgagc atgttttcgg
     9781 ctttaatgct ttttccccgc acaccgtaaa gaggaactcc gttaacttct acaaacaaac
     9841 gatccaaagc atataattta tctttgagat gaacactaaa agtataggta tcgttttgtg
     9901 tttcaataag atcggttttc aaatccggaa tggtaatttc cggtacatta aagcttcgtt
     9961 ccgactcaaa attcaccgga tcaaaaccca ttttttgcaa tcgcttcaaa taggcatttt
    10021 tgtaagttcg aataagttcc ggcggggcct tgcctaaacg ttccagaaca atatccgggc
    10081 gattgtactg taaatcaaat tgatcaaaat tataaatttt tttcccgcta ctataataaa
    10141 tatggtcggt aatatttttc gtggccatat aataaccgtt atcaacgacc gtcacccaac
    10201 cattcccttg atcaaacccg atgaacttgg ctatttcttt tttgctctgc cgattccaca
    10261 aacgtacgga tccgtcatta cccgctgata aaaaaaattg ttccttattg atgaaattca
    10321 actgattgac ttggccgaaa tgccctctat atgcaccgat tttttttccg ctatctaagt
    10381 cccactccat aattccttcc aacgggtcac tttcattgta acacgcgcct aaaaccgatt
    10441 tattatccga tggcaagagc gcagatcgaa ccgttccatt catcacaaaa gattgtcgga
    10501 accgaccggt attaacgtcc atgaggtaat ttttaatata gaatccgaag aaagccgttt
    10561 tctcatcatc ggataacgct atactgtagt taatagaacc gggaccagcg tacaattttt
    10621 ttattttttt agtctgccaa ttccaccata tagggcactt attgtatccg cctatcatta
    10681 gcgacccatc tttgaagaac tgaatttgat gtaccgaatg cgtgtccata ttcgcaccga
    10741 agtatttatt cggatttgta aggtcaaata tttttagatt aggtctatca tatcccgtca
    10801 atcctccgac ggccagcgtt tgcccatcat gagaaaatcg tagtgcattt ataaaatcag
    10861 ttttgtcttc atcggacgtt ctgaattgat tgatcacgga gttagatacg atattcagta
    10921 tatgaatcgt atctccgatc gccgcagcaa gaagacggcg attaggatta aatgcataat
    10981 gacgaaccgg tgagatcgca tagttatact gcggtttgcc gtacgtcaac gaccacactg
    11041 aaacaaacga tttatatccc acaataattc tttcttcatc cggatctgta aaaatagcca
    11101 ttgccggttc tgttttacgt ccaagcatcg ccgtggacga atccccgtcc atatgcagca
    11161 aaaccatact ggtattccat tctgtcccgc aaagtaagat ctttttgttt gcactatagc
    11221 ctaataccgt tggcgctcca agttttgaag tcaaatcttt atcggtcagc ggagttgtaa
    11281 ttccgttcac tatatccgtt ttaaagataa gaccgtcata ggatgccgac atgagttgat
    11341 ttcgttcggc gaaacacaag gccgtaatat gcttcttgtg aatatttatt tcagacttcg
    11401 ttttcttttc gttcaggtca aacacgaata attttttatc gtatgtacct gccgccgcca
    11461 acaaaccgtc gttcgaaatg cagatatccg ttatttggcc caagacttga ttattcccat
    11521 cgcgcagaac cttaatttcg gattgcgtac tgaaatccca cattcgtact gtaccttttc
    11581 gatctccggt caaaacagtt tgaccgtgca cggcgatacg cgtgattttt tcttcggatc
    11641 ggcaaaatat tccgatttcc gcaccggaca tggcatcata cattttgacg gcatattctg
    11701 cgattactaa gaccgttcta ttatccgatc caaattgaag cccgcgaata gaaaggttac
    11761 ctcccatttg actcctaccg agcataataa catgcacaat gctgtcactg tccagatccc
    11821 atatagacac attcccgtag ttatctcccc atgcggctaa gcgttgatcg gatgaaatat
    11881 gtacggacat cggaaaattt aatacgccat cgctgttaaa attggtaaac cctttacgcg
    11941 aataaatttc gcgcccccga tgtatatccc atagtttgag cgtattgtct ttactgccgg
    12001 tgacagcata acgcccgtct tccgtcatcc aaaagcatgt cacgcccaac gtatgatgcg
    12061 atggtatcac aatacgcaca ttgtgttgcg cattggcaaa gctagtaaac aacaaaaaca
    12121 acattacctt cagaagtaaa taccgcgtgt gatgcatagc gctacctaag aatttataat
    12181 tacaaagtat cgggtttggc gaatcaactg atgagaggac tttcatagag taacctgaat
    12241 caaaaaaaaa taggcgcatg aaaatgtacg ttttactctc tgtaaatcaa tgacaaatgt
    12301 aagaaaataa catcaaagaa aactcaaaat acagaaaaac gattctaaga aaaatgaata
    12361 cagctataag tgaattatag tccacttatg cagcgttata ttgcttcgga taacattgca
    12421 ttatcttttt cccgcaaaca aaaaacctcc gtttttgcgg aggttttata gcatcacatt
    12481 gtacaaaaga ctactgtcca ccaccttgca tgatgatggt cgcacgggct ttggcggctg
    12541 tatcaaatgc gccaaaatag gcacgcagtt cgtattcgcc ttcacccaag gttacactgt
    12601 ttatttcgac aacgccgtct ttttctatcg catacacgta ttcaccgcgt tcggtatctt
    12661 ttttcccttt aggtacgacc gtaatccaaa cggacggact gccgggaaaa ttaccatact
    12721 ccaccgggat aatctggcca atgagataat gtgaattttc agtacgtaca tacggcccgg
    12781 tcggtttttt tacttcttct tttgccgcta aaacaaaaat gctgttttta agtacgactt
    12841 ccgctccgcc ccaaccgata ttgcaggcat tcgcccaaag tttgccacag tgttcgccgt
    12901 ccttatacgc atgtttggcg atatacaatg gctctttttt agccgcatca ttatagccgc
    12961 ccaaaatagc attggcaggc gtcgtacctt tgtacgcgac ccaggttacc gcattggccg
    13021 gtgcggttag gatttcaaaa tctttatcat aggaatattc tttaccgccc catcctatat
    13081 tacaataccc gccgcctata acttttccgg gatgcatccc gccctcaaat gctacacgcc
    13141 cgacatacat cggttgaccg ttttcgtaac cgccgacgac cgcattttcc ggaattacac
    13201 cggtaaaagg aacccatttg gtcaaagatt cggtcgatgg tgtcataccg gttacggtct
    13261 ttttaacagc attcgaacct cctttggctc cctgcgcata gccattcaca gcaacgtgca
    13321 gccacatgaa taacgcggcg caaacaagca ataatgaaaa tctgttgaat cgcatgtacg
    13381 aatactcctt agtaagtaaa aaaatggtag aacagtaaaa cggatgatct cgtttatggt
    13441 cgaaagcgga atgaatggta gaaccgatta cacgaatgga agatagcgta gccgttagtc
    13501 aaagtaaaca atcaatatga gatatgcatt ccggtgccgt atcacatcag ggcgatgctt
    13561 attttttcca atcactagca ctctctctca atccggtatg atctgcctcc cctctgttgc
    13621 gcacgcaacg tgctgacttt tgtgcaacaa atcaaaagta tcgccgtaaa cctctattcg
    13681 actaaccgcc tttcaaagcc cattaggtac tcatacggca tcatgatcta gcgatgaaaa
    13741 aaaataccga tttttctcga ctgtaacttt ttcacgccgc ttgacaagac ggattgctac
    13801 gtttatcgaa tttccctcgt aaacacatag gttcgtttgt ttattaccac caatgaaagg
    13861 atgatgagat gaagttcagc aagctactac cgttagttat ggcgtttata acgatttcgt
    13921 tttctatttc gtacggtcaa acgtggcagc ctgccggagg cggaagtaaa aaagccagcg
    13981 tatcggaatt gataggactt accaacgtca cggtcgaata ctaccgtccg gcggtcaacg
    14041 gacgagaggg taaaatttgg ggaaacatag tccattatgg ttttgcggat ctgggatttg
    14101 gcacgacgaa agctgcaccg tggagagccg gggccaacga caataccact atcgaattta
    14161 cgacacccgt ttttattgag ggcaaaccat tgccggccgg aaagtacggt ttttttattg
    14221 ccatgagtga aacaaaagct acattaattt tttcgaaata taataccgca tggggaagtt
    14281 tttattatga cccgaaagac gatgcgctac gcgtggatgt acccgtcata aaaaccgccg
    14341 aaagcgtaga gcgtttgaaa tacgaattca gcgatcaaac ggatgacagt gctgttcttt
    14401 cgctattgtg ggaaaaagta aaaatccctt ttaaagtatc cattgatgcc aaaaaagaac
    14461 aaatcgaagt ttttcgtaaa gcgttcaatt cggcaaaatt ttatgtgtac tggcaaaata
    14521 tgcatgccgc ggccaattat tgccttgtca ataacatcaa tcttgaggaa ggtatgggat
    14581 ggtctgagcg atctattaat gattttttcg gcgaggccaa cttttcaacc ttatccacgt
    14641 acgcgggatt tcttgaaaaa tttaatcgta agaaggaagc ggattcggta atgcaaaaag
    14701 catttccctt gggcaccgca cttcaaatat attcctacgc acgcagtctc acgaaaatga
    14761 aagaaaatca aaaagcttat gaggttttca aaatcggtta cgataagtat ccgaatgatc
    14821 cgaatattat cttaggaatg gccagaggca atgcagcgat cggcaatatc aatgaagcgc
    14881 ttaatttttg tgaaaaagcg ctgactttgt ttgccgatcc ttcatccacc ggtattatca
    14941 aaaaaatgat ggaagacctc aaggccggca aagatatcaa taaataatca tcatagaagt
    15001 tcaatacaaa gaaaccgggt acagagaata gcgaggttca aaaatcgggt atgtgaaaac
    15061 tgaattttaa tgaggcgttt attttttaca ataatcactc aaattcaata accatttttt
    15121 ctacgatcac ataacgatcg gattctaatt tgatcatacc cggttttttt accgttacgt
    15181 tgcgcgtaga gcgttgcgga ttgtaggtgt attcgcacaa cggctgaagg tagcgatcat
    15241 gaaacgtgat cgcctgcgtc atgttttcac tgccggtcca gatagttacc gtgccggttt
    15301 gtttgtcggc attcagctcc actttgaata acgaatcaaa acccggcgac actttgaggt
    15361 cttttttgcg gataaatccg tcggtggttg atgccgtcac ataaaaaact tccttgtctg
    15421 aagcggccgg ttcggccggc gtcggtgcga cgaccattga acgcaaatat tcgagttcgg
    15481 ctttgagcgc tttaatctgc gtttcaaaag attttatttt cgtcgtgtca tctgtacccg
    15541 cgcctttttt agccgctcgc tcttcctgaa tatgttccaa cgcacgcaca cgctctttga
    15601 gtttaccaag cgaacgggac atcccagcta tcaataatcc gcctcctaag gccatcagca
    15661 cgagcacaaa agctgtcgcg tacaacatga tttcaacgat cgtcatgatt tattccttta
    15721 tagttcgcac atcggtcgta cattctatga ttttacaatg gataatccgg aactgatgga
    15781 aaccttaaaa caagcttagc ctacttattc ttttacgaac gtcgcgcgca tttttttaaa
    15841 gcactttgga caaattgtaa aacgcatgca cgagcggata aaaaaacaaa gtttctttta
    15901 cagggtgatt ctccatagtg ccgtctaata atacctgatg acttattccg tcgctgataa
    15961 ggtgtataat atctttttgc agttcggtgc ggcacatggc caaatcggct tctatgccga
    16021 aatattcgtg aaaagatatt tgctcgttca aatcaaaaaa tagatttatg aaatctttga
    16081 tattatcctg gacgtctttt ttgactccgt cggatacggc gtcgtacgtg agacctttgg
    16141 catcattttt cttttttccg ccaaggtgga tcatagcttc gatcggctgc tcgttttctt
    16201 tttgacgcac gataccgccg atgcaggtgg cttgtttagg ctctttggaa acgacaaggc
    16261 gcatctcttt ggctccgttg ccatagacgg cttccgctaa aatacgggta aacgccgtaa
    16321 gattttttaa cgccgggtcg cgatcaataa aacctaaaac gcgactgcca ttaccgctga
    16381 aacagatata atgcggcgct tgcaattttt tggtgcgcat acactgcaac gcgtgatata
    16441 ccgtggcggc atagtaagcg agaaaaataa tcttaaattt ctgatcctta cccaaacgtt
    16501 cgctgatctg cgtaagatgt tcatgtttaa aaaacacctg catcacgtcc tccgaacgct
    16561 tgcgttcgtt ggcgagatac tgttcacaaa tatcccgcag ctgattccat tctttattgt
    16621 taggcggagc gttgcgaaaa accgcttgat acgcttcacg gaaaataccg ttgtctttat
    16681 tattgacccg cgaggccagc acattgcctt cgctccataa cacgttacag ccaaaattaa
    16741 acgacgaacc gaatttgggt tgacgttttt cgaataccac gatatccgtc gaaccgccgc
    16801 cgatatccac gcacagtaca ttgctgtcac cgacgaccgc ggtcgtcgct ttgtgataat
    16861 aatacggtgc ttgcgactcc gtcatttcta ttacggcttg ttcggaaatg cccagcactt
    16921 tttgagctaa ttgcgtccaa tcgcttttga gcaatccgat cgcatgcgcg ctcatactca
    16981 gcgggtgaaa ccatatgagt ttggttttgg ccggatcgcc ctgattgaga acgactttgt
    17041 ggcgaatcag aagtaaaatt tcttcaaaaa acgcacggat ccgttccgtg gttttttcgt
    17101 cgagattgat tttccatttg atattggtgt gtacgatttc gtcgtcaaac tccacatgcc
    17161 gctcgtggtt aaacgccata ttgatattac cgagcgtaaa cgtctcttcg cgtacaaatc
    17221 cgggcgattc gctcgtcgcc gtacgaatcg gaaactggat cggagaaccg gcagcaccga
    17281 taaacgtcgg cacaaaatcg ttttcctgat ataccgagga ttgcggcaat acggtgcgat
    17341 cgttgtagcg gcgatataaa tcggtcaccg catcacgatt ggaaattttt tcaggtccgt
    17401 acaaattgac caccggcata tcttctttgg taaaatcaaa aggtttcgga gcgcccgtgg
    17461 catctgcata cgcgatgtgc gtagacgttg taccaaaatc cacggcaaac gtatatgccc
    17521 gcgttcccgg ctcgacgtaa cgccacatcg gcacgatcca tcctacggct ttttgcggac
    17581 ctttgagttc gcagcgaata taatcaaatt gagcacccgt aatctgataa tagacgccgc
    17641 cgtcaaacac ttttttatca tggcgtacaa tcttcttcac gggcgattgt ttgcttttac
    17701 tttccgacag cggctccatg cggctgtcaa aaaaagtcaa atccggaaaa tcttttcggt
    17761 ttcgctgccc gtccaccatc atgacattgt accgctcgac ggattccgct tcctgtttca
    17821 cacgataaaa aggaaaaacc gctacgtcaa atttccactc cacgatacgt ccggtgtccg
    17881 tgccttcgat cggatcgggt acataccggc gtttcagttc cacgcgtttg cccgaacggg
    17941 tcggtatctg caaccgcacg gtcacggcat ctccgtcaac cgtaaacgaa atttgtttcc
    18001 taaggtcttc cggcgaaaaa tagttataat aaaataattt taccggaagc atatacgatt
    18061 cgtcttcatc gctgtgcgtg agcgtcaaaa acgaaggatg cgctttgtac ggtaacttga
    18121 tcagcgaatt ttccaataaa tccgaagcca acaaatacgg atatggtatc tgcgtaccgg
    18181 gcaacgttcg actttccaaa ggattcggat ccgtcaccgg tacttggcga cgcacctctt
    18241 cattccagtc cgcatcgacg tagatcacac ccggatcggt aaattgtttg gacaatacca
    18301 tcggtatcaa accgtctgtg ggtttggtca catccatcag aaagccgctg cgctcggata
    18361 tccggttagt caaatcccgc gcacatcgcc atccgacctg atgcaagtaa agtttcagat
    18421 ttttgggctc ggtgtattcg gaacgaaaat catgttcgga aaaaccgttg gcataaatcg
    18481 catccacggc gatgcgcagt tcgcgatctt tgatccatgg tcggttttta tccacatagt
    18541 cgtgaaccga acgatagata cctgaaaatt cacgatgccc cgctatcaga cggtgtacga
    18601 acatctgaaa atcgcgatcc cgttcgtgca gcgccaacgt tgtttcaaaa tagcgtgtgc
    18661 cgttggtgcg gcgaatatca atcgttttga gcgtagcatg atgcggcacg gtaaaaaaca
    18721 tagtcaacgg cgatgtcgcc gcaaaaacac gatggttgta cgcgatcaga taaaaccggt
    18781 cgaagctgcc aaaattagcg gaatcgcgat cctgttcgat aaacagtttg agtgtttcac
    18841 ccaaaatgcg atgaccatcg gaacgcgatg tgatgagtcc gtcaatctga tgattaaaaa
    18901 cccattcttt gacttccagt ttaccgccca ccccgccgat attattcgca tgaaagagca
    18961 gctccataac atcaaacgta taggagatga gccgatgata gagtgaattc ccgctcattt
    19021 cgccacgccc gttgacggag cgaaatgcgt aatcgaataa atgcatctgc gcaaacggag
    19081 aaggaataga cgcaatggga cgctcgactt tgtcgctctg attgtcttcg atagaattaa
    19141 tcatattggg atgaaacacg gtcgtatccg tccagtgatc ctgcgccaca ctgcgtgccg
    19201 gtttcagatt gagaagatat cccatgcggg ctccaaaacg ttattgaatt tatttataaa
    19261 aacgttgtat caccgcgtcc gtcgcggcat aaccggcttt gatcaatttg cccatcgtcg
    19321 tcagcgtggc cggcaactga cggctattgg atcccagcgc cgagatgata aaattatcgt
    19381 ccagtcctgt tcggaaaagt ccgcccggct ccagttgata accttttacc aaggcgtgag
    19441 caaatgactc ggcaaactgc tgcatataaa acggctcaaa agcgcgtttg ttttcggcca
    19501 actctgttat ccagtcggca tacatgtaca aaaacttttg cagcaatttg gcatcttcgt
    19561 cattgagcgc cgatgccgta ccaaaaatat tttttatcca ggcatctttt cgctcggaac
    19621 gcagcaaaaa ttgcatgata taataaagat acaaaaaccg gatcaacggc gctttgatca
    19681 acgcttgcgc cggatcggga agattggtaa aggtcagtac ttcgccgtct tctttgaggg
    19741 caaattcatg gcataaggtt tttgcgccgg atgtttcaaa cgtctgccgc gcaaaatcaa
    19801 taatagccag tgcgcctaaa acctcaatca cgtgggcttt gttacgctgc ccggtggatc
    19861 cttcaaaatt gggatacaac tctttgatcg tatcggctaa ataatacaaa tgatgcaaac
    19921 cggagagatt tttttcgtaa tacgcaagcg cggctttggt tttagcatga aacgtattcg
    19981 aatcaatttg actttcgttg tccgcttgta cgccaaagta cggcatgacc gtcagcgcac
    20041 ctttcagcgc tttctgcagt ttgggatcgg atttttgcga acaaaaattt ttgagcaaaa
    20101 tcggaaaacc cgccgcaccg gtaccgccga aaatcgaact gacgacaaaa atacgatctc
    20161 ccggctgaaa ggaattgaca aattgatcgt attcatccat tgaggtcagc gtattgagcg
    20221 ccaccgaacc catattgggc ttgcctttga agccttccgt cagttcgtca tccaaatact
    20281 cttccgaata caaaagattg accataaact ggtcttccgc cgagagtttg ctgtaatcta
    20341 tgaagtcgcg aaattttttc tgatgtgtac tgtcaaaact aaaacgaaac atcgccgtcg
    20401 ggtcggcgcc cgtgatctgg ccgatcgtgc gcatcggtgt cgtaaaaaac tccgattcgg
    20461 gttgtacgcg tgatcgtatc ttttgatacc atttcaacgt ttcttccgat tgtaccgtat
    20521 ttccattggt cagatccaca tccacgatga tcggtacgac ttcgtcggct tcgatgcgca
    20581 cacccgacgc gagcagcatc gtcaacgaac gaataacccg tgcgccggtt ccgccgatac
    20641 caaatatata cagtttactc atgcgataac tccgtcagaa tggaatcgtg gaagcattgg
    20701 tagaataacg tttcatcgcc aaaccggcta cgagactgag gataaacgca taggccatat
    20761 tggccagcat aaacagcatc gcactgccgg gtaatccgtt gatcgttgta ttccatttaa
    20821 atacacccaa cgcgatcaga ccgaataaaa aagccatcaa tccattatac gaccaaaaaa
    20881 accgcgtatc gacggctgtt ttgggaatac gaagactgag aaaaaaatag tacaaccagg
    20941 ctgaaaacac accgtacagc aacatcaaaa accccgcaag cggatagatt tccctccgcc
    21001 atacggcttc cagttggcga ccgtgccaca cttcgtataa ggccgtaaac atatccgaca
    21061 tgacttattg ttcctttacg atattaagct tcactgaaaa atacggcccc gtatcgccgc
    21121 ggtaagcttc tctaaaaccg tgaaccaaag cctgcatact cggatcgctg ccataaaacc
    21181 gttcaaaatc ctgtgccttg gccaattcaa aaaaatactt attcgtattt tcaaaaaggc
    21241 gatgaagctt gaccacgaca acgtgcgaat atgtactgag ctgctcggct tctttttcgt
    21301 cagcaaggtt tttaaactga tgcgccgtgt accaacgcat cgcaacgtgc tgcggatcga
    21361 catctttgat caacgtgttt ttagttaaat aaaccgaatc gagttgcaaa ccttcgtatt
    21421 tttttaagtc caagcccatc gcaaactgga tatacccttt gccggccagc gaagcgtctt
    21481 cgatcgttcg gcaaaaatcc tgactgataa accacgtcgt ttcatcgcgt tgggaataat
    21541 tcaaaaaagc tatttccggc accggatatt tcacacccag aaaaatttta tgccggtaat
    21601 cgcgcaaccc gccctcccga tcaaagagca gtacggacgc cgacgtgccg atcaccatca
    21661 cgtaatacgg gcgatcaaca gacttttcac gaaaagcata caatcccacc gcattatgtt
    21721 tggctatttg ggcaagtacg tcggttttta aagcaagatc gagattgtcc gcatcttttt
    21781 cagacaattc aaaatccgaa accaaaacgg atatgtcctg cgcttcatgc cggcgttgca
    21841 cctgttcgat catggataaa atcggtgtgg acttggcttt cataatgtcg ccggtacgga
    21901 tagccttata caaaatatta taatcagtat gcaataaact gcttgcgatg agatgataac
    21961 gtaccgtgtc ggcgatgtta tcggttttca ttttctgaat ccggcttata cattccgata
    22021 tggttgaacg aaacagcgaa gtgggattgg taaaatcacg catggaaccg gagttgtcca
    22081 cgtacattcg taccgccgcg ataggaagcg gttccggtaa cgttgccgaa tctacgcacg
    22141 gacagccatt ggcatctacg gccacaccgc gtgcattatc gcattcgtcc ttggcgtctt
    22201 caacccgatc accatctttg tcagatttgc atccgaacaa aacgacggaa accacgatca
    22261 aagaggtcaa acctatgacg cgcaaccgat gaaaagtctt tgtaaacggt accatgaagt
    22321 agcaatcagg catttgataa accgatgtcc gttaagatta agaatcatac accgaaaaaa
    22381 caatacaaaa cacgacgcaa cgggatttgt tgatggatcg tgcaaccttt tgcaaaacct
    22441 ttccgtatgc attttatccg tcgcgctttt gttgttattg acttttgcgc gtacgctttt
    22501 tattatgtcg cacctttcga ttggaaaatg cagccaatct tgtttaaaaa aattgtccca
    22561 gtcttatgaa ttgaacgccg gattacatat ccccgcgttg attcgcgcga tgccggtatg
    22621 ctgtacatag gctaatgtac acaaaaacgc aatgaattcc gttatagtac aattcaattt
    22681 tttcacttaa cgaacagagg tttatgagta caaacaaaga agcatggggt tcgcgtgtag
    22741 gcttgatcct tgcgatggca ggcaatgccg tcggactggg aaacttccta aggtttccgg
    22801 tacaggcgat acaaaacggc ggcggggcct ttatcatccc gtatttggtg tgttttcttt
    22861 tgatgggatt gccgctgctt ttcgtggaat gggccatggg acgttacggc ggtaaaaacg
    22921 gccaccacag cacgcctttc attttagatt caatggacaa acgctcattt tggaaatata
    22981 tcggcgtgtt cgggattttc accaaccttg ccgtagcggc gtactattgt tatcttgaat
    23041 catggaccat cgcgtatgtt tatcacatgc tcgtcggcag ttttcaaggt caaaaccagg
    23101 atgaagttgc cggatttttc aatcactatc tctcttttga aggttttgag aacgttattg
    23161 tttggcttct gtgtttattc cttaatacct ggatcttatc caaaggtctg tcgggcggcg
    23221 tagaaaaagt tgccaaaatc ggcatgccgt tgttaattct tttcggcatt cttttggcca
    23281 ttcgcggatt tacacttagt gccggggaaa atggcgctat atatgacggt accgtcggtt
    23341 taaatttctt atggacgcct cagtttgaca cgatttggga tggcaaggta tggctggccg
    23401 ccgccgggca gatatttttt acattgtccg tcggtatggg atcgatccat tgttatgcat
    23461 cctacgtcaa atccaaagat gatattgctc tcaattctat gtctgcgggc tggatgaatg
    23521 aatttgtgga ggtggtgctc ggtagtgcaa tattgatccc tatcgccgtc ggatatctcg
    23581 gcatcgacaa agtcatcgaa atgaccgcca gcggcgggct tgggttgggt ttcaaaacat
    23641 tgccttacct ttttacgcag tggggtccga tccttgccgc attggccggt gtgttttggt
    23701 ttggtctttt attttttgcg ggaattacat cctccctcgc gatgggtaca ccctgtatgg
    23761 gtttcatgat ggacgagttc ggatggaagc gcgaaaaagc cgcatgggca ttcggcttta
    23821 ccatactcat attgggatta cccaccgtat tattttttca ccacggggtt ttcgatgaat
    23881 atgattattg ggccggcacc gtttcattgg tgatttttgc tttattcgaa attatcctct
    23941 ttgcatgggt ttttggtatg gataaaggct ggaaagaaat caccgacggc gccgatatta
    24001 aagtacctaa cgtattcaaa tttatcatca aatttatcac accgattctt ctcggatggg
    24061 tatttgtgaa gagtcttccg gacatttggg atacaataag caataaaaag ctatatgata
    24121 aaatagctgc cactacggat actgtgcaaa tcgctgcaat acaagaatcc atcatgtatg
    24181 tcaatgcctc gcgatttgta ttgctgggca tttttgtcgg agtatctatt ttagtattta
    24241 tcgcatataa aaaacgtatc cgcgaaggga gatttacatc atgaattcat ccgcattagc
    24301 gtttatgctg atcacctaca tcgtagttat cggagcgaca ggatacttct tttatctcgc
    24361 actgacgacc aaaccgcatg acgaaccgga ttcttttacg gaaaacgaca acgtcccgcg
    24421 gtaatcaagt aaatactttc atctataaaa gcgtatgctc atctcgagcg tacgcttttt
    24481 ttattgggcg aaaacaaatc gcatttctcc tgttgtacgg catgatgcgg ttcgctatat
    24541 tgcgccgatg ttattctcaa taaatggaca atcaacgtgc atctaggata tcttttcaaa
    24601 gaaagtttca gcggtttgcg gcgcgcgacg ttatctacgt ttgtcgccgt gacgatcatc
    24661 gctattgcgc tgtcgttgtt tagttttttt ctgatcatca cgctcaacat caatcaactc
    24721 gtcgaagaca tacgtagtcg cgtggaactg gaagcgttcc ttgaccgcgc tttggacacg
    24781 ccgcaatcta aaaaaataga agccgccatt cgcgacatcg aaggcgtcga atcggtgacg
    24841 tttatttcaa aatccgatgc ggcgcgaatt ctcaaaaaag ccatgggtgc ggatgatatt
    24901 tttgatctcg tagaaagtaa tccgctcccc gcctcgttca aaattcgtct gcaatcggag
    24961 taccgcaatt ggcagaatgt gctcgccgtt gcccagactc tagaaacgat tgacggcgtc
    25021 gaagaagtca gctaccaaaa agaactcctg ctcgctttgg atgaaaaagt agatttgtat
    25081 aatatgatca gcatcggatt gggctgtatc gccgcactgg ccgccgtact gctggtatcc
    25141 aacaccatca aactgagcat ctatgccaaa cgcgatgtga tcaaaacgat gaaactcgtt
    25201 ggcgccaaaa acagttttat cgctgcgccg ttcattcttg agggcatcct gcaaggtgtt
    25261 ttgggaagcc tgatcgccac cggtatagct tatgccttga tccgcatcgt ccaacgttat
    25321 cttttaccga cgttgcagtt ttcgtgggaa ataccggcca tcgtggtcgg ctttggtatt
    25381 ctgctcggct tattcggaag ttttgtctca attcgttttt ttctgaagga aaaaatcagc
    25441 gacatgtgat tgatttatga acgaaaaaag catgcctcgt tgcatcacgt cctctctttt
    25501 tattgcgctt atactgacga cggcttcgct ccgcgctcag gaagaaactg gctatccgta
    25561 tatcaaaaac tacaccatgc gtgatatccc tgccggctta caaaattggg ctgtcgctca
    25621 ggatcaacgc ggcatcatgt acttcggcaa ttccaatggg ttgcttattt ttgacggcat
    25681 ccattggcgc gtcacggaaa cgccgggccg cgcattcata cgttcgatcg ctatcaatac
    25741 gtccgatgaa gtttttgcgg gtgcagtaga cgacttgggc tacttatggc cggactccaa
    25801 cggacagcgc caatttgtat ctctactgtc tgccatcccg gaaccggatc gccattttgg
    25861 cgatgtgtgg aaatcgtttt gcataggaaa ccgcatcgta tttcaaacct ttagccgcat
    25921 ttttattttc gaatccgcac cgccggaatc gcctgaacga tttcggttcg tacgcaccat
    25981 ccaaccgccc aattcgcttc atttctcgtt tcaagttcga aatgcgatct atatgatcgt
    26041 acgaggccac ggattacacc tgttgcaaaa cgatacgctg gtactagcgc cgggcggagc
    26101 gttttttgca tcggatcgga tttacgcgat gctgccgtgg gaaaacgatg caacgatgat
    26161 ttttactcgg gaacgcggca tttttctttg gaaggacgga aagatcgcac cgtttgcggg
    26221 cgacgttcat accgatctgc cgcggcatca gatataccac gcgacattat taagcgattc
    26281 gacggttgcc atcgctacgc tggataacgg gatatggatt atgaaccgtt tcggtcggcg
    26341 cattgccgca ttcgatcgat catccggtct tatggatcaa tccattttat atctttttac
    26401 cgatcgtgaa aataatttat gggcaggtct caatcaagga attgcacgtg tcgagtaccc
    26461 ttcccgttat acttttttct ccgaacgtaa cggtctgacc ggctcggttt tcaaaatcgc
    26521 ccgtcatcag ggaaatctct atgtcgcaac gtttgacggc ctttaccgct tatctaaaaa
    26581 caacacgcat caactggagc gcgtaaccgg aatttcttcc tccgtatggc acttgtactc
    26641 tgccggaaca tcccttgtta ttgccgctac ggacggtgtg tacgctttgc gggatcatgt
    26701 cattcagcga atcagcgatc ggccttataa ttatgtgatc gcccccacgc tgtcggatgc
    26761 caaacaaatt ctagtcggtc atgccaaagg agtggatata tatcgtcaac agaacgggag
    26821 atggatttcg acggcggcgt ggcaaggcat tgatgatgag atacgtacga tcacagaata
    26881 tgcgccggga aaattttggt taggtacgat gtttcgcggg gtgatgcatt atacaacgcc
    26941 gggacaggtc acgcgttacg gtgcgtcgca tggtttgccg cccggtgaaa ttcggccttt
    27001 catgatcaac gaacgcttac tttttgcaac ctcatccggc attatgacgt ttgacaagga
    27061 tcgtttccta cgccctcccg cattgcaagc acccgacgat gtctttgtag gttatggtgt
    27121 atggatgcat gccgttgatc gtcataattt ctgggttagc gtagaaacta agcgcggcgc
    27181 gaatacctac cgcgccacga tggatgataa cggatccatc cactatggga aacccatttt
    27241 ggtccgcgcc gatcgccacg tgatttggaa tggtatgcag gacgacgacg gcatagcctg
    27301 gttcggcgga ccggacggta tcattcgtta tgacgcccat acggccgaca acacgacggc
    27361 tccgtttcat gccttgatta gccgaattac tttgcaaagt gacaccagcc gagccgaata
    27421 caatatccgg ttccgtaatc ctacacagat accggaatat ccggcaatgg gcattcggtt
    27481 tgaatgtgcc gctacgagtt ttacccgtca ggaattcaat gaatttcaat attggctcga
    27541 aggtttcaat catatgtggt cggattggac aaccgagacg cgccgggatt ataccaatct
    27601 gccggaaggt gtctttacgt ttcgcgtgcg tgcccgcaat tttgcaggtc agatcggaaa
    27661 agaagatgcc tttactttta gagtgacggc accatggcac aaaagcaatt ggtttatgag
    27721 tctgaccgca atggtgctcg ttggtttttt gatcggagcc gtacgttttg ccacagtgcg
    27781 acgatggaaa cgccgtgtga tggatctcga actgcgtgaa cgcatttcac gcgatctgca
    27841 cgacgagatc ggcgccaatc tgagtaatat tgcgttgctg tcagaaatgc tgagcgctaa
    27901 agaaaaattt gaaaacaaag aaaaagagca actggcccgc atcagcaatg tcgcacgccg
    27961 aaccgtagat acgatgcgcg atattatttg ggctattcaa ccgcatcagg atcaacttga
    28021 cgagatgatg ttgcgcatga aggatttgac ctccgagttg ttgggagata ccttgcctta
    28081 cacactcgat tttccgtccg aaaacccaac ccgtaccgtt acgatgccct atcggcaaaa
    28141 catcatactt atctacaaag agcttcttca caacatcatc aaacatgcgc aagcctcgtt
    28201 cgtacacatc cgtttggtta ttaccggcga tgaactcgtt ttggttgtta ccgataacgg
    28261 catcggtttt gacgcccagc attcccatga cggcggatac ggcgtaaaaa atctgaagcg
    28321 gcggacacgg ggaatccgcg gcgacatccg ttgggaaaca gccgaaaacg gcggaacacg
    28381 aaccatatta cgatgcactt tggcgtaatt gggacgggaa tttttgcttg atattccacg
    28441 gctcggtctc tatattgcgg cacattcatt atcaaactaa gcatgattat ccaccgatac
    28501 ctccgtcaaa aaccagctat tccgaaatcg gtttttttag cgaccggtgc cattattgtg
    28561 ggtgacgtcg ttatgggtga agactgttca gtttggttta atgccgtgat tcgcggcgat
    28621 gtgaatacaa tacggatagg ttcacgaact aatatacagg acggtgcaat tattcattgc
    28681 acgctggata aatatgccac ggtgatcggt gatgatgtat ccatcggtca tggcgccatt
    28741 gtgcacggat gtaccgttca cgatcatgtc ctgatcggta tgggcgctaa aatactggat
    28801 cgcgctgtga ttcacccgta tgccatgatc gcggcgggtg ctgtcgtccg agaagggttc
    28861 gaagtaccgg aacgcacatt gatggccggt gttcctgcca aacctgtacg atctctgacg
    28921 gacgacgaaa tcgcgatgat tgatcgtata ccgcaaaatt atatacgtta tgccgcggat
    28981 tacagaacat taaacgtttt ggattcagcg gttgtttttt cttcatcaca aacctcaaat
    29041 gtatgaaaaa aatcgttatc attgacgacg atcaggactt tgtttcggtc gccggtaaag
    29101 ttctttccca catcggtaaa tttgacgttg tggcgcatac cgacagttcg gacgcgatgg
    29161 atttgattcg ccgtgagaaa cccgacctca ttatcgtgga tatcatgatg cccaaagtgg
    29221 acggttttac aatccttcgc aatgttcgta cctcagccga cttacaacat atcaaagtca
    29281 ttgtgtgttc agctaaaatc tttgatgtgg ataagaaaaa tgcgctcaag ctcggcgcca
    29341 atgaatatat ttccaaaatc accgccgctt cggaactggt ggacaaagtc aatgccgtac
    29401 tcggtacggt ctgatctttt ttttacaagg gatatcgtat gaaatacaaa ttttggggcg
    29461 ttcgcggctc gatcccaacg cccggtccgg aaaccgtcgg ttacggcggc aatacctcct
    29521 gtggcgagct gacactcgat gacggcagca tgatcatttt tgatatgggt tccggtatcc
    29581 gcgtacttgg taacgatttg atgaaacgcg gtaaaaagcc ggtcgaggcc atcattctct
    29641 taagccacac gcactgggat cacattcagg gtttcccttt ttttgtaccg gcttttattc
    29701 ctggcaataa aattttgatc tgcggatgtg aagaagccga catcaaattg gaaacgatca
    29761 tcaaagatca gatgaaaagc gcctattttc ctgtcgaatt gtccgacatg cctgccgcta
    29821 taggatttaa gcggctctat gaaggacgtt tcaaaatcag cggggcgcgt gttgatacca
    29881 tgtatctcaa tcaccccgga ttcaccttgg cttaccgcgt ggaatacaac ggcaaatcgg
    29941 tcgtctatgc cagcgacaac gaaccgtatc ccattcagcc caatgattca ccggaaatgg
    30001 ccggcatgaa atatcagggc aaagacaacc atcgcattat tgagttttcc aaaaattgcg
    30061 acctcctgat tcacgattgc caatacacac ccgaacagta taaaaccaaa gtcggttggg
    30121 gtcattcacc ttacgactat gtcgctgaaa tcgcatggaa agccaatgta aaacgactgg
    30181 ctgttcacca tcacgatccg agccatgatg atgcctttgt ggacggtatc gtcaataatg
    30241 tgaaaaacct gctccgcgaa aaaggcgcac gcgccgaagc attcggggca cgggaaggtt
    30301 tggaaatcga actctgatgc gcgaagaact gtgccggttc ctagcgctca aaatatagtg
    30361 taatcggtag cttgcgtcac ctgaagatag ggcgggtgta gcatcatcat ttaaaacgac
    30421 aggagtccgt atgaacattg tcgaactcga caatattgta ctttacttag acaaacaagt
    30481 gctcggtaaa gaacctaata tttctctaaa agacctcaaa tctaaagaca tccccagttt
    30541 ttataaaaac tatttcgatc gcgaagccga aaaatggatt cgcgaagaat cctccatgtt
    30601 tttatcgtcg ggtcgtttta attacgatga tcccgaggtg cgcgctaagt ttgaagaact
    30661 ttatatgatc ctgaaaaata aggcaatctt caatcggtcc aaattacacc gtaatatgga
    30721 gcaggctgtc aaactgcaat ccaatttcct tatcgcgccc cagcgcacga tggtgcagtt
    30781 tatttatcgc gacgatgaaa cgctcacgcc ggcggaagtc aaagattcga tgaagtattt
    30841 tcttgattac gattattatt acaaagcgtt ggaaacctat tttgccacaa acgctatgga
    30901 gaccatttcg cgagccaagt ttaaaaactt catcgctcaa ttggacgaaa aggcgctgtc
    30961 gcaggacaaa acggttgccg ccgtcaa
//
LOCUS       DAOQGF010000031        30811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_662_length_30811_cov_7.324327, whole genome shotgun sequence.
ACCESSION   DAOQGF010000031 DAOQGF010000000
VERSION     DAOQGF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_30811_cov_7.324327"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1070
                     /locus_tag="PK937_06360"
     CDS             192..1070
                     /locus_tag="PK937_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNH32227.1"
                     /translation="MRPAVELQKNEVWSDACAKYIIKILGAFIMLRWLAIIIMTAIFI
                     SSCEKDKNSSDPVNDMVLIGQGYDQGVLVSVYSEDTLTTGYNAIYTRLQDSASGEILD
                     EGHIHLSPLMHMTEMTHAAPYGNPESEEAEEGLFEGYVVFQMPGNDMEYWELEVEFHE
                     HHSGKEGSVTIEPKVKSSNRCKVVTGTDAKSYILSWKKPRVPEVGLNTLELMVHERVS
                     MMSFPDVTDATLAIEPTMPSMGHGSPNNVDPVYVSGAVYRGKVNFTMTGDWQVDVSIT
                     RNAATVVETHFDVTVD"
     gene            1159..3396
                     /locus_tag="PK937_06365"
     CDS             1159..3396
                     /locus_tag="PK937_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH32228.1"
                     /translation="MKPLLMIIKYCTGLVVLVTLSVQAQEHATMVHGRIVDEETREAV
                     RYAHIRMDSTEEGTIADSAGYFHLPRHHGKYKLRISCVGYQTAWVSVDHFSEKRIMPV
                     FLRSNAIRFEEEVIVSAPWIEQQQESYYLEKKLNTTDDILEKTDGLSLIRRGNFGLEP
                     AIRGLSGGQIQVLVDDMKIFGACIDKMDPVTSYVEIDNLEKIEVAKGGFDMSAGQTIG
                     GSLNLRTGKPDFSVPFAIGVQTGYENVSHLRFARSTTNWSGGAWAARAGVSLRKSDDF
                     RAGDGRLMHNSGYNKSNIKTDIAHQNGNHTWTASYIRDVATDAGYPVLLMDTRRAVAN
                     IFGLEHQWRQPWHHASFLNTKVYFNMVDHDMDDYDRDVTARDFMPNMYMPMYGNTRTI
                     GFSSSLYTGGNIQGIKWVVDFYRMTAFADMDMQSLDPEVSPMYLVNIGSTHIYNYAVA
                     AEYRRLLTEKIVFKSSFRLDYSVRSLHDDIGRRELEGYWNRTHLNTSYITFSASAAAE
                     WQMMADQKLRLALARTARTPTHIENYGFYFYNVSDGFIYTGNPNLKPEVSYQSELSYE
                     YKTDKHISKAQIFYTRIDRYIAGITTDTLFKQIENIHHADLAGIEASHFRSITNTIAW
                     QITASYRYSRVSAWDEPMPGMAPLEVLNSVRWVPGSYWFELSNKTAASQNRFAKQQAV
                     EDRTHGFTLFTIRGGIKVNKHAELQWGVENVLDTDYREHANIQKMPGRGRNVYLTLQW
                     NHWAK"
     gene            complement(3407..4627)
                     /locus_tag="PK937_06370"
     CDS             complement(3407..4627)
                     /locus_tag="PK937_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HNH32229.1"
                     /translation="MRRLFLFLLIVSGTTAHGQFSSYRMAADLDSVRQNGWHHLHILP
                     SWLALMREDKADVRLFSFRKNDTLETPYLMRKSEDRMRTKEIPVHVINRGLSGRTLFL
                     TLSQPSKKIINSLTFSPEAINFNCGVLIEGSDDQKNWIRLATDEPQRIFGIQNSDISY
                     RYTTLRFTETNFRYLRLQLSDASQLGTVHVEATENTVDWAELDTLRITSTTTTENHTA
                     HTTEMIMEFENPSAVDRLQLRFSNTGDFYRSVEVLARGDSIGGRNGHNAWWYPVSSGV
                     VSSLEPAILRNLSVRTSAIKLVIHNHNDQPLKITNAMVFAERTYLAAKLEKNTRYVLT
                     FGKKNDQAPRYDVVYFQETIPSVLPIISAGANFSLQSENNVTTFGQNWWIWVLLIVAA
                     VILGWMAMGLIKEK"
     gene            complement(4620..6950)
                     /locus_tag="PK937_06375"
     CDS             complement(4620..6950)
                     /locus_tag="PK937_06375"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNH32230.1"
                     /translation="MADPKSFEALEQRLNHITQTLIAAQQEIGRLRSEIQTLRAESHP
                     ASTPLTVEEKSHTVLPTPVTTAYTIAKPAVSEIPKSKISAEEPKKNIEAFIGGKLLTI
                     IGIVVLVIGLGILVIAAIEADMIGPIGRVMIGILSGLGLLVVAKKLKRTYELFSAALL
                     SGGMATLYFSVFAAFSFYALIPQWAAFVMMLMLTIFTVLAATRYDRQIIGVMGLVGAY
                     GVPFLVSTQSGNILVLLSYITLVNAGILILGFRKNWRIMNHTAFVLTWAIFTAWLVDR
                     YDEKTQRPIALGFSTLFYIKFYLLFIAYKIRSKEPFAAYDIIRLLGNSFVYYGVGYGI
                     LDSVSHGAYQGLFTMGIAATHIAFGYYLRKTQNGERALVLAVAGMTLIFTAIAIVVQL
                     EGHWVTMLWLAVASVVFWMGRSQNEVWYVRMAVALYIIGFISFIHDQQHVVLVNTDLV
                     VVWKPVFNLNFLSALWAIGAFAFSQYIIFHPSYVTKNAPPSQIDRIGLMIISGLTALT
                     VFFAFAYEIHFYFQSLYRQTYTDWHDIARADISFLYYNTAWISLYTGLFLYAFTVLIC
                     RFYKDWKGFIAASVLAGFYLILVLTQTVDAMGELRSFYIAPPGEYPFVVGFSSMLIRF
                     GVWSAVGLLLYGISSYAKAAEPKFSEPLHVIINICVIIILSLELKNAWLVTHADDTSL
                     LATQVYRIGFTILFGIYSLTLIAIGIWKRHRMMRYFAIALFALTLVKIYTYDMQNLSS
                     FNRILLFIPLGLILLLVSFLYQKFKHIITGDEKTNA"
     gene            7224..9194
                     /locus_tag="PK937_06380"
     CDS             7224..9194
                     /locus_tag="PK937_06380"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNH32231.1"
                     /translation="MVIIFFVLTAMALQNCDEHRSRNSSEDDFFGHTLDTTFAHAVTI
                     AYSDSSISVINPFDTLGVTISIDGGDVVVNSTITGTEIQYVLSGKISDGMFKIYSVYK
                     LALVFNGVSITNDNGPAVNVQSSKKVSVNIVYGTNNRLIDGASYITTSEDQKATLFSE
                     GQLSFSGTGSLKVKGNYKHGIAGDDYIYIKESNIDIIAAASDGVHANDYFTMESGYLH
                     VVASGDGIECEEGFIAILGGHITVNSIDEGITASYEDGDISVTPYIQITGGTIHVTTS
                     GAKAHAIKSESHTTINSGETIMLTVLGPGAKGIKTGGNLTLINNSITITTGGNAYYDA
                     SASDIVSSAGIDCDGYFLMEQGTLTVTSSGTAGKGINIDSTMEMNNGTISVSTTGAGY
                     IYGSLTSEAKAIKCDGAITINDGTIMVSSADDGIKSETALTINGGTVTVTKSYEGLEA
                     PLVTMNNGNVSVTASDDAVNTTYGNGGEAVDGSLLTFAGGTIALNSSNGDAIDANGNI
                     EMTGGVVIAQGPKVYPEVAIDCNGNFNISGGLLIASGPNAGPMIEATSTTSTQYTALI
                     KIISNVSPGRLFNIQDSEGNSLVTYAPSRVAYYFVFSSPLLQNGATYKVFTGGNCTGA
                     TVTNGLYFGGTYSGGTQKGSFTISSKVTTATL"
     gene            9453..10631
                     /locus_tag="PK937_06385"
     CDS             9453..10631
                     /locus_tag="PK937_06385"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNH32232.1"
                     /translation="MSDFKVAPASAMSRLGTETAFEVLVKAKALEAQGKHIVHLEIGE
                     PDFDTPTNISEAAIKALREGWTHYGPSPGLPDLRKAIADYVSKTRHIETHPDEVVVTP
                     GGKPIIFFPIMALIDPGDEVIYPNPGFPIYESMINFVGGKAVPLHISEEKNFTFDVSE
                     LKKKISHKTNLVIINSPANPTGGVFSKKDIEDIAAIVKQYNCYVMSDEIYSRIMYGYE
                     HYSIASLPGMKERTIILDGFSKTYAMTGWRAGFGVMPKDLAAMVSKLQTNSNSCTNSF
                     VQKACIEALQGPQDSVNAMVTEFEKRRDFIVDGLNAIKGFSCKKPLGAFYVFPNITKT
                     GLDSRKLADYLLNEAGVAALSGTAFGSFGEGYLRFSYANSLDNIKLALERIDKAMKKL
                     "
     gene            10719..11834
                     /gene="gcvT"
                     /locus_tag="PK937_06390"
     CDS             10719..11834
                     /gene="gcvT"
                     /locus_tag="PK937_06390"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNH32233.1"
                     /translation="MSTSTNPQKTPFYDKHIAAGAKMADFAGYYMPIRYDGDIVEHQR
                     VRTAVGVFDVSHMAEFMVTGEGAADFVQKITVNDVSKMADTQAQYSAMCKPDGGIVDD
                     LIVYKYNDKKFMLVVNASNMEKDFNWVKSHCPSHLQLENISDDIALLAIQGPHAKEVV
                     QSLTKTNLSDIKFYWFREGEVDGVPMTISRTGYTGEKGFELYFNTKFADQIWNAVMKA
                     GEKYNIGLAGLGARDTLRLEMNYRLYGNDIDETTNPLEASLGWITKLNKGEFIGREAL
                     QKIKADGVKRKLVGLEVEGKIPARHGYDIMSDGRKVGVVTSGTFSPSLQRAIAVGYVE
                     TALAKDGTMLKINARGRELNAKVSPTPFYNGDPSSDF"
     gene            11878..15039
                     /locus_tag="PK937_06395"
     CDS             11878..15039
                     /locus_tag="PK937_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNH32234.1"
                     /translation="MASKKKNNPKPKAAESPKGKIPQKPQPPKGLKLNLGIKLKEEIF
                     GLLLMTVSLLSILSVVTHVANEQPGTIMELWRAGQLQNLLGPVGAMLSHYLVGYTFGY
                     PFVAVPILFLVYGWLFFTHKSIFLVNQFAFYILTLMLIVSTWLAIPGALDHSENWNAS
                     GMLGGMLAHQLYTWLGGFGSVTIWVVFSLIWITMVTRVSIADLIPAIKNILFTSYRTT
                     TGTISKFKSRQQELLEKENEVSDDELDKVNEDATEKPLIPEYLQKKTIKTSVSPTSAK
                     TEKVNEPVKTEIPQVTTKQSTTIAPAIKEEKAPEESVAVQAKAEEPKREAITGTDLGM
                     DTQFVRDLKAIGHIDKKFSINRTKLEELSASAGHTDSHQDLPQEETTAPANTPVTENP
                     KPRVVEPAKDLTIRPEEADLDMADADFKDLARSMFDAEHQHIEWEKEWVKEETAELER
                     AKTRLDMKPARKLKTQTVKIEDIVETEEAPKEDIHNIVPESTDASQLSTNDINPVTQE
                     EPQPEVPLPETAQEAEALAASIKAVKKEAPKPEPAIDFDKENSQARKKYKFPSIDLLE
                     PTQSLEKLTPEEIGYLNTKVEQIIAKLAEFGIATKVVATEYGGPVVAIYQLELPSGLK
                     ISKITGLEDELALSLKVKSVRMVPVTSKGTIQVEIPKLKAEPVMIRSLFEDPSFRDAK
                     NKYKLGLALGKTIDGHIHFEDLAKMPHLLIAGTTGAGKSVGINSIITSLLYQFDPSEV
                     KFIMVDPKKVELALYRRLKNHHLISLRNHRGEVIEDVITKPENAKLILKALVEEMEER
                     YEKLAEANVRNLEDYNKRWKEGRMPHDGKYQHYKLEYIVAIIDELADLMMTAPREIET
                     SITRLAQMARAVGIHLIVATQRPSVDVLTGLIKANFPARIAYAVRSKIDSRTILDMGG
                     AEQLLGKGDMLFMPPGQVPIRIQNAFTSTQETETIVDFINRMQAFPRRDFMVKEEPRE
                     ENEGGEVDSSGFDELFNQAMEIVVKHNQGSASLLQRRLSVGYARAARLIDQLERAGIV
                     SAPDGAKPRQVLITEDQMAMYMN"
     gene            15096..15731
                     /locus_tag="PK937_06400"
     CDS             15096..15731
                     /locus_tag="PK937_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNH32235.1"
                     /translation="MKYLIVSLMALSGVWAQGKPEDAQSVIRKVQNKYKKTDSFSFSF
                     DYRFKWKLTGKSQSAQGKLFFKKENNIRYELGQQLNITNGESVWQYSEVNNQVIIDKL
                     KKNSRSLFMPKYFLYDYLEKFIAEVIKTETINGRLVYVLKLDPRDRDDFVQSMKIWVP
                     SDTWITQKIEYADLEGNQVTYEFTHVEIDQPMNMNLFTFTPETGMDVIDLR"
     gene            15744..16742
                     /locus_tag="PK937_06405"
     CDS             15744..16742
                     /locus_tag="PK937_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNH32236.1"
                     /translation="MKKVLFLLLNVLVSVFFVWLATRGIDWTQLGSSLTSISLTLVIF
                     SVIINQFSLWLRAWRWKFLLAPLKDVPTLSLFSNVSIGFMINNILPFRLGEIARGYIL
                     KKEHQISFTASMATVVVERVIDVISLLTVFGVLVIIFPFPEWIKTAGMVVGAIDLAAI
                     IFMILLVKNTEATCRLVQSMISVFPDSLKQRVDRLLRSFVSGITFTHALGTTVVIAML
                     SFLIWAVYTVSIWIMFKACNAPELATLDLIDATIVMTFSSFAVMIPAAPGYVGTFHEV
                     VKQSLMMFRVDREVALGFAIVIHAFNYAAQIVIGVGYWIKTNFSLKEALSNVHEEK"
     gene            16729..17712
                     /locus_tag="PK937_06410"
     CDS             16729..17712
                     /locus_tag="PK937_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNH32237.1"
                     /translation="MKKNNFLKMLIIGLLMVMSMSSSAGELHAQTKDSLKTDALSQIR
                     ADRKIRPGDILDVVVYGHDELPKQIKVEADGTIKYPFMKDIKVDGMSVEQLTNILSAR
                     LSQYIGGRAEVIVAYAKDEMIEVIVLGSVAQPGSHKIPRNHTIQGAIATAGGATVRSD
                     LDRTKLIRINPETSLREEIEVPMVSIIVETGDIRKLPALRDGDVIFVPSIYGAVYANV
                     LGAVGKPGNYELFPGANLVDVIFLAGGPKDDASIRTVKLIRRIGVAQTEEVVDVEAVL
                     KAKGGSVPLVQPGDIVFVPAKKFTLKTIFQVLSYAATILTLIFLSKQAGLL"
     gene            17739..20000
                     /locus_tag="PK937_06415"
     CDS             17739..20000
                     /locus_tag="PK937_06415"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNH32238.1"
                     /translation="MSDLNDDKQGIDARAILQTVLMHKWIVLTVFIVFSGIVSYYSLS
                     KPTLYRSSALLFFDNGLSNLAFANVPQQQKIALGSQDFGYYEVIITTDLFNQRFRKEL
                     ERVVLSERNDAETVLALKNVSSTQVTLKPYKEVEQFIEISAVNYDSFLVKKMIEVTTD
                     VLKSRVTELDREGLQRSIEFIDEQVEVSRNNIEKTEVELQALQKKTDVNIKQSEGPLA
                     KVLIMKEKLAEFETQIQIRSSNINALNSQLDSIQRRLTGSSSYAANESTEEKQIKLQI
                     EQLTSQKAVLYEKLGSDAVENPEVQRIDRQIADLRSQYYTLLSSVKTGSDNTVSGDMS
                     QVWKTIWDKKNTEEIELFLLKGQARLFSGLITNFERKNPNLLQDAIDISRLLRLKQVY
                     EERLSSLIKQKESLSIQYYGSTGSLKIIDPAKEPVAIYNKVFTNIFVGTVLGLIIGIA
                     IAVGLEFMDNRIKSIAAISSVTSIAVVGRIPPINVGDKDNAVEGLRKKINKIKTKDEE
                     EDKNEIRRKAMISQFNPRSFVSERYRSLRTNIQFANIDSPIRSLLVASAGPGEGKTTT
                     AVNLAISFADMGHRVCIVDTDLRKAKHHMLFDIQEQPGLTDIILGQTDLDTALQKTSI
                     NNLHSLTVGRDAKDHSEIFSSLRMNFMMNELEKKFDIVVYDTAPVMLLTDSVILASKV
                     DAVLMVVKHAVTPKSQLQNAIHNLKGVRANIIGIVMNDYADERKNYYKYAYDGYYLSK
                     SQRISTSEEGVKA"
     gene            19997..20944
                     /locus_tag="PK937_06420"
     CDS             19997..20944
                     /locus_tag="PK937_06420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012862519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH32239.1"
                     /translation="MSKILVTGCAGFIGSHVTDALLKAGHEVVGLDNFDHFYDPAIKE
                     KNLTNAIQSPGFRLLRGDILDVDLMGRIFSESKFDLVIHLAARAGVRPSIQDPKIYQK
                     VNVEGTMNILEAMRAHNVKKLLLASSSSVYGNNEKVPYHETDNVDHAISPYAATKKAC
                     EVLAYTYHHLFGIDAFCLRFFTVYGPRQRPEMAIHLFTRLIDEGKPITLFGDGQTFRD
                     YTYVDDIVHGIMQCIDGLRGYEILNLGESQTISLIDLVNTIEKALGKKAQIRWMPNQP
                     GDVQKTYADISKARHLLGYKPSVGIEEGVRRFVDYFRST"
     gene            21012..22568
                     /locus_tag="PK937_06425"
     CDS             21012..22568
                     /locus_tag="PK937_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH32240.1"
                     /translation="MDNARQKDITKLAKKFAISGIGSIFGLFWTPLTSVITTRFLGAE
                     LYGIFSLVQSWGSLLANLSSIGLNGMNLRFIPTYKATGSMALIKGSVFTTIRVSVVIS
                     AAATCFILFFPDLFCELFIHRPETISEEYFSNYVTKAFQFYAVSILLTASYQAFLSSL
                     VGYQFVQYKVLANDVLGPAAKIISLTSLLLLDFNLYAALGSNIIQDIVVLLVSAFYLF
                     RIAPELRDKSIPVQIEKKKMMKFSSALFANSLLSKYTYQLDILFLGYYATLPEIGIYT
                     LALRLQPLIYLPHYSISSVFGPMVAELYTAGKIKELNQFYKTTTKWSVTLSLPICSLI
                     ILFSKEILGVFGKEFAAGFELVAILSIGNLIHDFLGLSGNIILMTGRIRINVINSIVM
                     AIINLLLMSILIRNYGMYGAAIGNFLSITILSILTTVETFYILKIHPFDFLLLKPFFA
                     MLISSSIVIAISIWYRLPYYEITFLIYILVFILLYVGFLFLFGLSQDDRMILDKILKK
                     LPFKKQNSNE"
     gene            22561..23265
                     /locus_tag="PK937_06430"
     CDS             22561..23265
                     /locus_tag="PK937_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH32241.1"
                     /translation="MNDKHHYDGISVDQYDNRKNRNVFEKYLYDHWHPIIVEHIRLLC
                     DKRDVLDLGCGSGAYIGFALESTCKVSGLEVSQNMLNAAKKRYPKADFILGDAQETRV
                     ESESFEIIYSIGLLEYVNVENTLKECYRILKSNGRLMILTPNKYGGRKFVTRYLKGKK
                     KSSPKYYSRREVLNALDKQGFYIDKVIMDDGLIYLPNITPDIISRLIFRTNETFFKLF
                     PYNPFSQNMIFIATKR"
     gene            23262..24515
                     /locus_tag="PK937_06435"
     CDS             23262..24515
                     /locus_tag="PK937_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32242.1"
                     /translation="MMGNDAEINEYSLQNLRKYFLVSIQNYLEILMLMLLVPIVWLIS
                     NPYDFIASNDPWEYFNISHGLIIGNGIVLNDNYDVILPPGYPVIIGLLYFLIPVEWPI
                     LLGIGIVSFILSIILMKKILQLMGFNFHLRIIGLFFFAFNSQLLIQAGRGYSDLLMIL
                     LILSLIWVSIIKKNTKNLSWGIVLGMLWGLLSLTKPEGVLLGLVIISLSVFNSRMIKG
                     IAAICFIVLYLPYGVFLKNQTGAWQISGKTYVNLVLGELNSPYQNPTNYDTRYAVISR
                     VWENAEEAGGTSVYLINLGYKILERVPYNIKRFSNYFVSTYSWIGGCFLLACVIFWNS
                     IYRRFIVLSIGFTCFYLFFFIIPRVLTLYHVFFSFAMLAGFQIILTHINRVRNRWLTL
                     SFYGAVVFFISFQFRIVLKILMQKI"
     gene            24556..25560
                     /locus_tag="PK937_06440"
     CDS             24556..25560
                     /locus_tag="PK937_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32243.1"
                     /translation="MRRTVFFIVSIDTECDKSIDWSITYPFSFRNIHEGVAEKLQPLF
                     DIYKVKPTYLLSPEVIYNQESVNYFKSIQDRCELGTHLHGELIGYDKLENPRWTNDFQ
                     CDYDEGIEREKLESLTELFKNKFGYMPKSFRAGRFGIGPNTWRILAELGYAVDSSLLP
                     FQLIKTKNATYNFYYTPVKPFYPDLQNWMNFEKQEIRKILHVPMTVHNWFFQRIPPCI
                     GKPLSEQNRIRSLFRILLTKEKEKTYSLRPRTDNAESLINLIKHHIKIHTTKEPIVLN
                     MMFHSNELHPGASPYAQSPLEVGQTINTIKRILDYINRYYSIHSIGLCDSTRYYERDT
                     "
     gene            25557..26783
                     /locus_tag="PK937_06445"
     CDS             25557..26783
                     /locus_tag="PK937_06445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH32244.1"
                     /translation="MKILHIISNGNIGGREKVLYLLLNEESKDKQIQTSAFFKYPVGP
                     YYTKIKSLGISVYYNITRLRDIISLMEKQDIIVFHFVDWRFFLAGILSGKSILYRLSG
                     IYLLNKKSLGDVFFILAKKIKKTIWRRHSEDDRRGDQLIKLPAKENTSQSKIPNRRSL
                     YRLIKRKYFELFLRKWCTYLIANSRYTAKSAVEKYGIQPQKIRIVMNGIEFNNRKGDE
                     TDFRASYNIDKNVFLIGTVCRFDTRKRLDRLLMGFSMISNLKDFHLVVVGGGDTVIEQ
                     ALKQFVAHNNLLDKVSFTGILENSSDAMSAMNLFVLSSDNETFGVSLVEAMYKKKPVV
                     VFSDSGGPAEIISGQNCGYIINTPSEMPGIVEQLRADPILTQRLGENGHRLVVEKYNV
                     KRFYKNSLRVYSELFE"
     gene            26804..28657
                     /gene="asnB"
                     /locus_tag="PK937_06450"
     CDS             26804..28657
                     /gene="asnB"
                     /locus_tag="PK937_06450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNH32245.1"
                     /translation="MCGIAGVMNFTGEPVNESLLKAMGDALAHRGPDGDGFFIRQHVG
                     LAHRRLSIIDLKTGQQPMYDDDGKSCIVYNGETYNFGEVREELIKLGWNFRTQSDTEV
                     ILKAYLQWGASCLDRFRGMFALAIWDERTKSLFLARDRVGIKPVYYYADQNKFLFGSE
                     IKAILQDKSIDRTIDYSAMREYLQYGYVPSPASIYNKIKKLPPAHWLHITFDGGRYNM
                     KSKCYWDIQFGQDTDGAHNDTTWIDKTESVVSDSVRIHMISDVPVGAFLSGGIDSTIV
                     SHFMAKHVGTINTFTVSMNESRFDESAYARQVSAIIQSRHVEKSVQAADLNTILSNLV
                     YAYDEPFADSSAVPTYQIAKIIRSHVTVALSGDGGDEVFGGYKRYEQLLEWSQSNPWL
                     NKVWLSKIATFWPRNIPGKNYLRYHSQSDMIRYYGMINIFHSDQIADMMKFSVEHRER
                     LFDSYMNSSDALLNKMQNIDIHTYLPENGLTKVDRASMAHSIEVRVPLLDHKVIELAA
                     QMPVSMRMRKSEKKYVLKKILEPRFPKELIYRTKRGFSLPMRDWFKSNPDLTRMIHHE
                     IKECELIKEHMNLHFIEAMITTHLNSSKNHASRIWSLWFLARWYDTYYKKM"
     gene            28679..29671
                     /gene="waaF"
                     /locus_tag="PK937_06455"
     CDS             28679..29671
                     /gene="waaF"
                     /locus_tag="PK937_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNH32246.1"
                     /translation="MEKILLSISAGIGDALMAQPLLCEIQNNFPQSHIEVLCSAATQH
                     VFRSHPGIQKIWTMDVSSIKKYFGLVGKIRKEKFDLYIGAIPSNTYSQLLLPVLAGIP
                     VRIKHNSPHKFPRNLDALYHALTPIGEGVHRLECNLELLHFLKVNHWSKQSPKFHISD
                     VTLAKMETRIAHDRKKLIIGFHPGCNPAAAFKRWPSERYAALADYLYTTYDATVFIVG
                     GKDEADDVRKIMEQAQHKPLNFAGQCTLEETAALIAQAHFFVSNDSGIMHLSTAVGTP
                     VFAIFGPKDERHIGPYGEKHTVIRNGRDVNQVTLEQVVQTLIASKFGLKPFIKS"
     gene            29685..>30811
                     /locus_tag="PK937_06460"
     CDS             29685..>30811
                     /locus_tag="PK937_06460"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNH32247.1"
                     /translation="MIEIRGARVHNLKNISLNIPRNKFVVITGVSGSGKSSLAFDTLY
                     AEGQRRYVESLSAYARQFLERMDKPEVDHIKGISPALAIEQRTLAKNPRSTVGTVTEI
                     YDFLRLLFGRAGKTYCRNCGALVRKDTVGYVVQRILALPERTKLHILFELAANEQTIL
                     SLHEKGFTRLIVDDVIAELNDEAVVKKILKQKIVRVLVDRIAVNKEEKARVADSVETA
                     FLNGEGKMIVQIMEGETLRFSNKFECNACGTAYIEPDPRLFSFNNPYGACPSCQGFGN
                     KIEVDMDLVIPDRTKTLKEGAIKPWTTDGYADFQERLEKAAKKNGIPLNIPVMDLTKE
                     QYEKVMQGGDGFDGIREYFKWLESKTYKMHVRVLLSKYRGYV"
BASE COUNT         9021 a         6068 c         7034 g         8688 t
ORIGIN      
        1 atttttcacc cgccgcgcgc ctaaactctt ttccaaacaa aggatgctcg gcgcatcgtg
       61 tttttcatac gatgttagac atccggttgg tacaaatgga tcatcgaaca gatactagga
      121 caataccctg agtacgcccg tcagtttcgt accggagcgt agtgtatcgt cacgatgaca
      181 ggtcaaattt tatgcgtcct gctgttgagc ttcaaaagaa tgaagtttgg agcgacgcgt
      241 gtgcgaagta tatcataaaa attttgggag cgtttatcat gttacgttgg ttagcaatta
      301 tcataatgac tgctattttt atttcatcat gcgaaaaaga taaaaattcg tccgatcctg
      361 tcaatgatat ggttttgata ggtcaaggat acgatcaagg tgttttggta tcggtgtatt
      421 ccgaagatac attgactacc ggatataatg cgatctatac gcgtttgcag gattcggcga
      481 gcggagaaat acttgatgaa ggacatattc acctgtcgcc gttgatgcat atgacggaaa
      541 tgacacatgc cgcgccgtac ggtaatccgg aaagcgaaga agcggaagaa ggattatttg
      601 aagggtatgt cgtatttcaa atgccgggca atgatatgga gtattgggaa ttggaagttg
      661 aatttcacga acaccacagt ggtaaagaag gttcggtgac tatcgaaccc aaggtcaaat
      721 cttccaatcg ttgtaaagtc gtaacgggaa cggatgcgaa gtcgtacatt ctttcatgga
      781 aaaaaccgcg tgtaccggaa gtcggcttga acacattgga gctgatggtg catgagcgcg
      841 tatccatgat gtcttttccc gatgtgaccg atgcgaccct tgcgatcgaa ccgacgatgc
      901 cgtctatggg acacggatcg cctaataacg tagatccggt gtatgttagc ggtgccgtat
      961 atcgcggaaa agtcaatttt acgatgaccg gcgattggca agtggatgtc agcattaccc
     1021 gcaatgcggc aaccgttgtg gaaacgcatt ttgatgttac cgttgattag tatgtagagt
     1081 ccatgcggct ttcggagagc ctcccggaag ccgcatgatt tgaaagtaaa aagtttcgct
     1141 aattcattga gtgtaaatat gaaacctctg ttaatgatca tcaaatactg tacagggtta
     1201 gttgttttgg taaccctatc cgtacaagct caggaacacg ctacgatggt gcatggtcgt
     1261 atcgtggatg aagaaacccg tgaagcagtg cgttatgcgc atatacggat ggacagcacg
     1321 gaagaaggta cgatagccga cagcgccggt tactttcatc tcccgcgcca tcacggcaaa
     1381 tacaaactgc gcatttcgtg cgtcgggtac caaaccgcat gggtgagcgt ggatcatttt
     1441 agtgaaaaaa gaatcatgcc tgtttttctt cgcagcaatg cgattcgttt tgaagaagaa
     1501 gtcatcgttt cagccccgtg gatcgagcaa cagcaggaat cgtattatct tgaaaagaaa
     1561 ctcaacacca cggacgacat tcttgaaaaa acagacggcc tttctctgat tcgtcgcggt
     1621 aatttcggat tggaaccggc catccggggg ctgagcggcg gccagattca ggtgcttgtg
     1681 gatgatatga aaattttcgg cgcgtgcatt gataaaatgg atcccgtcac gtcgtatgtc
     1741 gaaatagata acctcgaaaa aatcgaagtc gctaaaggcg gttttgatat gagcgccgga
     1801 cagacgatcg gcggatcgct caatctcaga acgggcaaac ccgatttttc agtacctttt
     1861 gccatcggcg tacaaaccgg atatgaaaac gtctctcatt tgcgttttgc gcgctcgacc
     1921 accaattggt ccggcggcgc atgggccgca cgcgcgggcg tttcgctgcg caagtccgat
     1981 gatttccgcg ccggtgacgg ccgtttgatg cataattcag gatataataa atccaacatt
     2041 aaaaccgaca ttgcgcacca aaacggtaat catacctgga cggcgtcgta tattcgcgat
     2101 gtcgcaaccg atgccggtta tcccgttttg ctgatggata cacgtcgcgc ggtggccaat
     2161 atttttggtc tcgaacacca gtggagacag ccttggcatc atgcatcgtt tttgaatacc
     2221 aaagtgtatt ttaacatggt ggatcatgat atggatgact acgacagaga tgtcaccgca
     2281 cgtgatttta tgcccaatat gtatatgccg atgtacggca atacgcgtac gatcggtttt
     2341 tcgagcagcc tctataccgg cggcaatatt caaggcatta aatgggttgt ggatttttat
     2401 cgtatgacgg catttgccga tatggatatg caaagtttgg atcccgaggt ttcgccgatg
     2461 tacctcgtca atatcgggtc aacgcatatc tataactacg ccgtcgccgc cgaataccgt
     2521 cgtttgttaa cggaaaaaat tgttttcaaa tcatcatttc gtttggatta ttccgttcgt
     2581 tccctgcatg atgatatcgg tcgccgggag ttggaaggat attggaaccg gacgcacctc
     2641 aatacatcat acataacttt tagcgccagt gcggccgctg aatggcaaat gatggccgat
     2701 caaaaattgc gattagcgct cgcacggaca gcgcgtacgc cgacgcatat cgagaactat
     2761 ggtttttatt tttacaatgt gtccgacgga tttatttata caggcaatcc caatctcaaa
     2821 cccgaggtca gttatcaatc ggaactgtcg tacgaataca agacggataa gcatatttcc
     2881 aaagcgcaga ttttttacac acggattgat cgctatattg caggtattac caccgatacg
     2941 ctgttcaaac aaattgaaaa catccatcac gcggatctcg cgggtatcga agcgtcgcat
     3001 tttcgaagca ttaccaatac catcgcatgg cagattacag cttcataccg ttacagccgc
     3061 gtaagcgctt gggatgaacc gatgcccggt atggcgccgc ttgaagtttt aaacagtgtg
     3121 cgttgggtgc cgggttcgta ttggtttgaa ttgtctaata aaacggccgc ttcacaaaac
     3181 cgttttgcca aacagcaggc agttgaagat cgcacgcatg gttttacatt atttacaatc
     3241 cgtggcggga taaaagtgaa taaacacgcg gaattgcaat ggggtgtaga aaatgttttg
     3301 gataccgatt accgtgaaca cgccaacatt caaaaaatgc cgggacgcgg aagaaatgta
     3361 tatttgacac tacagtggaa tcattgggct aagtaacgac cggtttttat ttctccttga
     3421 tcaaacccat agccatccat ccgagtatta ctgcggctac gataagtaat acccatatcc
     3481 accagttttg accaaacgta gttacgttgt tctcactttg caggctaaaa tttgcaccgg
     3541 cagaaattat cggtaaaacc gaaggtatcg tttcctgaaa ataaacgaca tcataacgcg
     3601 gggcttgatc attttttttt ccgaaggtca aaacatagcg tgtgtttttc tccaacttag
     3661 cggctaagta agttcgttcg gcgaatacca tcgcgttcgt aattttcaaa ggttgatcgt
     3721 tgtggttgtg gatgacaagc tttatcgcgg aagtgcgtac tgacaagttg cgcaggatgg
     3781 ccggttccaa agaacttact acaccggatg aaacaggata ccaccaggca ttgtgtccgt
     3841 tacgaccgcc gatgctgtcg ccgcgcgcca gtacttccac cgaccgataa aagtctcccg
     3901 tgttggaaaa acgtaattgt aagcgatcta ccgccgatgg attttcaaat tccataatca
     3961 tttccgtcgt atgggcggta tggttttctg ttgtcgtggt cgaggttatg cgcaaggtgt
     4021 ccaattcggc ccaatccacg gtattctcgg tagcttcaac atggaccgta ccgagttggc
     4081 tcgcgtcact cagttgtaat cgtaaatacc gaaaattggt ttctgtaaaa cgaagtgtgg
     4141 tatagcgata ggaaatatct gaattttgaa ttccgaaaat acgttgcggt tcatccgtcg
     4201 cgagtcgtat ccaatttttt tgatcatcgc tgccttcgat gagaacgcca cagttgaaat
     4261 taattgcttc cggtgaaaac gtcagagaat tgatgatctt ttttgagggt tgtgacagcg
     4321 tgagaaaaag tgtacggcct gaaagaccgc gattgataac gtgaaccgga atttctttgg
     4381 tacgcatacg atcttcactt ttgcgcatca agtacggtgt ctccagcgta tcatttttgc
     4441 gaaaagaaaa caaccttacg tcagctttgt cctcgcgcat cagcgctaac cacgaaggta
     4501 aaatgtgcaa atgatgccag ccgttttgcc gaacggagtc caaatcagcg gccatgcggt
     4561 aggaggaaaa ttgtccgtgc gctgtagtgc cggacacgat taataaaaaa aggaacaatc
     4621 tacgcatttg ttttttcatc gcctgtgata atgtgtttga atttttgata taagaaggaa
     4681 accaataaca agataagccc tagaggaata aaaagaagaa ttcggttaaa cgaactgagg
     4741 ttttgcatgt cgtaggtata aattttgacg agcgtgagtg caaataaagc gatcgcgaaa
     4801 taacgcatca tacggtggcg tttccatata ccgatagcga tcaacgtcag agagtagatg
     4861 ccgaataata tggtaaatcc gatgcgatat acttgcgttg caagcagtga agtatcatcc
     4921 gcatgagtta caagccaggc attttttaat tcgagggaca agatgataat cacgcagatg
     4981 ttgatgatga catgcaaagg ttcgctaaac ttgggttctg ccgctttggc ataggatgat
     5041 attccgtata aaagtaatcc aacggcagac cagacgccaa accggataag catcgaagag
     5101 aacccgacga caaaaggata ttcaccgggc ggagcaatat aaaagcttct cagttcgccc
     5161 atggcgtcta ccgtctgtgt taacacaagg ataagataaa agcccgctaa aacggacgcc
     5221 gcgataaagc ctttccagtc cttataaaaa cgacagatca aaaccgtgaa cgcatataaa
     5281 aataatccgg tgtataaact aatccatgcc gtattgtaat ataggaacga aatgtcggca
     5341 cgggcgatat catgccaatc ggtgtaagtt tgtcggtata gcgattggaa ataaaaatga
     5401 atttcatacg caaaagcaaa aaacacagtc agcgctgtta aacctgaaat tatcatcaaa
     5461 ccgatgcgat ctatttggga tgggggagca ttcttggtga cgtatgacgg atgaaaaata
     5521 atgtattggg aaaaagcaaa cgcgccgatg gcccacagtg cactcaggaa attgagattg
     5581 aaaacaggtt tccatacgac gacaagatcg gtattaacca aaaccacatg ctgctggtca
     5641 tgaataaaac taatgaatcc tatgatatat agtgccacag ccatgcgtac ataccagacc
     5701 tcgttttggg aacgacccat ccaaaaaaca acagatgcga cggcgagcca gagcatggtt
     5761 acccagtgtc cttctaactg aacgacaata gctatagccg taaaaataag cgtcatacct
     5821 gctacggcga gcaccaaagc acgctctccg ttttgggttt ttcgtaaata ataaccaaat
     5881 gcgatatgtg tcgccgcgat gcccatcgta aataacccct gataagcgcc gtgtgatacc
     5941 gaatccaaaa taccgtatcc gacgccgtaa taaacaaaac tatttcccag cagacggatg
     6001 atatcatacg ccgcgaaggg ttctttggag cggattttgt atgcaataaa gagtaaataa
     6061 aatttgatat aaaataatgt gctgaatccc aacgcgatag ggcgttgtgt tttttcatca
     6121 taacgatcaa ccaaccacgc cgtaaaaata gcccatgtca aaacaaatgc cgtatggttc
     6181 atgatgcgcc agtttttacg aaaaccgagt atcaaaatac cggcattgac caaagtaatg
     6241 taggagagca atacgagtat atttccgctt tgggtgctta ccaaaaaagg aacgccgtac
     6301 gcgccgacaa gacccatcac gccgatgatc tggcgatcat agcgggtagc cgccagcacg
     6361 gtaaaaatcg tgagcataag catcatcaca aaagccgccc attgaggaat cagtgcgtaa
     6421 aatgaaaagg ctgcaaacac gctaaaataa agcgttgcca tgccgccgct caacaaagcc
     6481 gcagaaaata attcgtacgt gcgtttgagt ttttttgcga cgaccaataa accgagtccg
     6541 gacaaaatcc cgatcatgac gcgtccgatg ggtccgatca tgtcagcttc tatggcggcg
     6601 atcaccaata tgcccaatcc gataaccaat acgacaatac cgatgatcgt cagaagtttg
     6661 ccgccgataa aagcttcgat attcttttta ggttcttcgg cgctgatttt ggatttgggt
     6721 atttccgaga cggccggttt ggcgatcgta taagccgttg taaccggagt tggtaaaacg
     6781 gtatgtgatt tttcttcaac tgtaagtggt gtggacgcgg gatggctttc tgctcgtaat
     6841 gtttgtattt cactgcgcag tcgcccgatt tcttgttggg ctgcgataag tgtttgcgtg
     6901 atatgattga gccgttgttc taacgcttca aaagatttgg gatcagccat gatggtattc
     6961 cggattggat gtgaatcatc caacgtacaa aaaaaattac atcgcctcaa tatttagtta
     7021 tgtgaaggag caatgtcagt gtttatttta tttggaaatc tgtgttaagc ttaagacatt
     7081 gccgacggga cgaagcggtc agcgtaatcg taaaaatcaa acttcttcac ttaccgagca
     7141 tcggaatgat gaacacgggt gattcaaata ttcttttctt tcacgcatta aaagaaaaat
     7201 cggtggtacg catgcggagg tttttggtaa tcattttttt tgttctgaca gcaatggcat
     7261 tacaaaattg tgatgagcat cgtagtcgta acagcagcga agatgatttt ttcgggcata
     7321 cattggatac cacttttgct catgccgtta cgatagcgta ttccgactct tcgatttcgg
     7381 tcattaatcc gtttgatacg ttaggtgtca caatttctat agacggcggc gacgtggtag
     7441 tgaattccac gatcacggga acggaaatcc agtatgtctt atccggaaaa atttccgacg
     7501 gaatgttcaa aatatacagc gtttacaaat tggctttggt tttcaacggg gtaagtatca
     7561 ctaatgataa tggaccggct gtgaatgtgc aatcgagtaa aaaagtttcg gtcaacattg
     7621 tttatggtac caataaccga ttgatagacg gtgcatctta tatcacgacg tccgaggatc
     7681 agaaagcaac actattcagc gaaggccagc ttagttttag cggtaccggt tcactgaaag
     7741 taaaagggaa ttacaaacac ggcattgccg gtgatgatta tatttatatc aaagaaagca
     7801 acattgatat tatagccgcg gcttccgatg gtgtgcacgc caatgattat ttcactatgg
     7861 agagcggtta tctccatgtt gtcgcctccg gtgacggcat cgaatgcgaa gaaggattta
     7921 ttgccatcct tggcggacat atcacggtca attccataga cgaaggtatc actgcgtcgt
     7981 acgaagatgg agatatatcc gttacaccgt acatacaaat caccggcggt acgatccatg
     8041 tcaccacatc cggcgctaaa gcacatgcaa taaaaagcga aagccatact actattaata
     8101 gcggcgaaac cattatgctc acggttttag gtccgggcgc caaaggcatc aagaccggcg
     8161 gcaatcttac attgatcaac aatagtatca cgatcacgac cggcggcaat gcttattatg
     8221 atgcatcggc ttcggatatt gtttcctcag cggggattga ttgcgacggc tattttctga
     8281 tggaacaggg aactcttacc gttaccagtt ccggtacggc gggtaaaggt ataaatattg
     8341 acagcacgat ggagatgaat aacgggacga tcagcgttag cactaccggt gccggctata
     8401 tctatggatc gttgacgagc gaagccaaag cgataaaatg cgatggcgca ataaccatca
     8461 atgacggtac tatcatggtt tcatccgccg atgacgggat aaaatccgaa acggcgctga
     8521 ccatcaatgg cggaacagtt acggtgacaa aatcctatga aggtttagaa gcgcctttgg
     8581 tgacgatgaa taacggaaat gtttcggtta cagcttcgga tgacgcggtc aatacgacct
     8641 atggaaacgg cggcgaagcg gttgacggca gtttgttaac ttttgccgga ggcacgatag
     8701 cgctgaattc atcgaacggc gatgcgatcg atgccaatgg caacatagaa atgaccggcg
     8761 gcgtggtgat cgcgcaaggg ccgaaggtat atccggaagt ggctattgat tgtaacggaa
     8821 attttaacat ttccggaggt ttgctcattg catcggggcc taatgccgga ccaatgatcg
     8881 aagcgacgag tacgacatct acgcaataca cggctttgat aaaaataatc agcaatgtat
     8941 cgccgggccg tttattcaat atacaggatt ccgaaggaaa tagtttggtc acctacgcgc
     9001 cctcaagagt tgcctattac ttcgtatttt cctctccgct tcttcaaaat ggagcgacct
     9061 acaaagtttt taccggcggt aattgtaccg gagcaaccgt gacaaatgga ttgtatttcg
     9121 gcggcactta ttccggtgga acgcaaaaag gcagctttac aatctcatct aaagtaacta
     9181 cagcaacatt ataaattgca tttaatcgta tcggccagca ccataacaaa aatcatatta
     9241 tcatcaacct ttgctttatg aaacggaggt ttttttatgt gatgtgatag acgacttaaa
     9301 aataagtacg agttacacgt taaataaagc acggtgatat ttatttttgt gtgcggattt
     9361 gcatgggaat aatttcttga taaaaccatt taaaaacggt aaattcttct ttattagaga
     9421 tttagcttta ataacacggg agagttttgg gcatgtctga ttttaaagtt gctccggctt
     9481 cggccatgag tcgtttggga accgaaacgg cttttgaagt gcttgtaaaa gccaaggctt
     9541 tggaggcgca aggaaaacac atcgtacatt tggaaatcgg cgaaccggat tttgacacgc
     9601 cgaccaatat ttccgaagcg gcgataaaag cgctgcgtga aggatggacg cattatgggc
     9661 cgtcacccgg tttgcccgat ttgcgcaaag cgattgccga ttatgtttcc aaaacccgtc
     9721 atatcgaaac ccatccggac gaagtggtcg tcacgcccgg tggtaaaccg atcatctttt
     9781 ttcccatcat ggccttgatt gacccgggtg atgaagtgat ttatcctaat cccggttttc
     9841 cgatttatga atccatgatc aattttgtcg gcggcaaagc cgtaccgctg cacatcagcg
     9901 aagaaaaaaa ttttaccttt gatgtgagcg aactgaaaaa aaaaatttcg cataaaacca
     9961 atcttgtgat tatcaattcg cccgccaatc caaccggcgg ggtgtttagc aaaaaagata
    10021 tcgaagatat agcggcgatc gtcaaacaat ataattgtta cgtgatgtcc gatgaaattt
    10081 attcacgcat catgtacggg tacgaacatt attccatagc cagtcttccg ggcatgaaag
    10141 aacgcacgat tattttggat ggattttcca aaacgtatgc gatgaccgga tggcgcgccg
    10201 gttttggtgt gatgcccaaa gatctcgcag cgatggtttc caaactgcaa accaacagta
    10261 attcgtgtac gaatagtttt gtacaaaaag catgcatcga agcgttgcag gggccgcaag
    10321 acagcgtgaa tgcaatggtc accgaatttg aaaaacgccg cgattttatc gtggacggac
    10381 tcaatgcgat caagggtttt tcctgcaaaa aaccgttggg tgcgttttat gtatttccca
    10441 atatcaccaa aaccggtttg gattcgcgta agctcgcgga ttatctgctc aatgaagccg
    10501 gtgtcgcagc tttatccggg acagcattcg gcagtttcgg tgaagggtat ctccggtttt
    10561 cttatgccaa ttcgctcgat aatatcaaat tggcactgga acgaattgat aaagcgatga
    10621 agaaattgta agaaaattga aagtagattg tcttttgtga attgactgat atagtaaata
    10681 agtcattttt aagtagtaaa gaatttggag aacatatttt gagtacttca actaaccccc
    10741 aaaaaacacc gttctacgat aaacatatcg cagctggtgc taaaatggcc gattttgcag
    10801 gctattacat gccgattcgt tatgacggcg atatcgtcga acatcaacgc gtgcgaacag
    10861 ccgtcggtgt atttgatgtg tcacacatgg ccgaatttat ggtaaccggt gaaggcgcgg
    10921 cagattttgt acaaaaaatt acggtcaatg atgtttctaa aatggccgat acgcaggcac
    10981 agtattccgc gatgtgcaaa cccgacggcg gtattgtgga cgatctgatc gtgtataaat
    11041 acaacgataa aaaatttatg cttgtcgtca acgcatccaa tatggaaaaa gattttaatt
    11101 gggtaaaatc gcattgccct tctcatctcc aattggaaaa tatcagcgac gatattgcgt
    11161 tgcttgcgat tcagggaccc cacgcaaaag aagtcgtaca gtcgctgaca aaaaccaatc
    11221 tgtcggacat caaattttat tggttccgcg agggcgaagt ggatggcgtg ccgatgacga
    11281 tttcacgtac cggatatacc ggagagaaag gttttgaact gtatttcaat acgaagtttg
    11341 ccgatcagat atggaatgcg gtgatgaaag ccggtgaaaa atacaatatc ggtttagccg
    11401 gtcttggtgc gcgggatacg ctacgtttgg agatgaatta ccgcttgtat ggcaacgata
    11461 ttgatgaaac gacaaacccg cttgaggctt cgctcggctg gatcaccaaa ctgaataaag
    11521 gcgagtttat cgggcgcgaa gcattacaaa aaataaaagc cgatggcgtt aagaggaaac
    11581 tggtcggcct tgaagtcgaa ggaaaaatac cggcgcgtca cggttacgac atcatgtcgg
    11641 acggacgaaa agtcggcgta gtgaccagcg gcacattttc gccgagttta caacgcgcga
    11701 ttgccgtggg gtacgtagag acggcattgg caaaagacgg tacgatgctc aaaatcaatg
    11761 cacgcggccg tgagcttaac gctaaagtat caccgacacc gttttataac ggggatccca
    11821 gttcggattt ctagagacaa ggcgtcttgg ttaaatatga attctaataa catttgaatg
    11881 gcgtcaaaga aaaaaaataa tcccaaaccc aaagcggccg aatcgcctaa aggtaagata
    11941 cctcaaaagc cgcagccgcc aaaggggctg aaactcaatc ttggcatcaa actcaaagaa
    12001 gaaatctttg gtttgttgct gatgaccgtg agtttattgt ctattttgtc ggttgtgacg
    12061 catgttgcta atgaacaacc cggcacgatc atggaacttt ggagggctgg gcaattgcag
    12121 aacctgctcg gtccggtcgg tgcgatgtta tctcattatt tggtaggtta taccttcggt
    12181 tacccctttg ttgccgtgcc gattttattt ttggtatacg gttggctctt ctttacgcac
    12241 aaaagtattt ttctcgtcaa tcagtttgcg ttttacattc tcacgctgat gttgattgtt
    12301 tcaacgtggc tggcgatacc tggggcgttg gatcattccg aaaactggaa tgcgagcggt
    12361 atgttgggcg gtatgctcgc gcatcagctg tacacgtggc ttggtggctt tggcagcgtg
    12421 acgatctggg ttgttttttc gctcatctgg atcacgatgg ttacccgcgt gagtattgcc
    12481 gatcttatcc cagcgatcaa aaatattctt ttcacatcgt atcgcacgac gaccggaacg
    12541 atttccaaat tcaaatcccg ccaacaggag ttattggaaa aagaaaatga agtttcagac
    12601 gatgaacttg acaaggtcaa cgaagatgct accgaaaaac cgctaatccc tgagtatttg
    12661 cagaaaaaaa ccattaaaac gtcagtatcc ccgacgtctg caaaaactga aaaagttaac
    12721 gaaccggtaa aaacggaaat tcctcaagtc acaacgaaac aaagtacgac gattgccccg
    12781 gcgatcaagg aagaaaaagc gccggaagaa tcggtcgccg tgcaggctaa agcggaggag
    12841 cctaaacgcg aagcaatcac gggaacagat ttaggaatgg atacgcaatt tgtccgtgat
    12901 ctcaaagcga tcggacatat tgataaaaaa ttctcgatca atcgcaccaa gctggaagaa
    12961 ctctccgcat ccgcaggtca taccgattcc catcaggatt tacctcagga agaaacgacg
    13021 gcgcccgcaa atacacctgt gactgagaat ccgaagccgc gtgtggtcga accggcaaaa
    13081 gatttgacga taagaccgga agaggccgat cttgatatgg cggatgccga cttcaaagat
    13141 cttgcccgca gtatgtttga tgcggagcac cagcatattg agtgggagaa agaatgggtc
    13201 aaagaagaaa cagccgaact tgagcgcgca aaaacgcgcc ttgatatgaa gccggcgcga
    13261 aaactgaaaa cacaaacggt aaaaatcgaa gatatcgtcg aaaccgaaga agcgccaaaa
    13321 gaagatattc acaatatcgt gccggagagt acggatgcat cacaactatc aacgaacgat
    13381 attaaccctg taacgcagga agagccgcaa ccggaagtac cccttcccga aacagcacaa
    13441 gaagcggagg ctttggcggc gtcaatcaaa gccgtaaaaa aggaagcgcc gaaacccgaa
    13501 cccgcgattg attttgataa agaaaattca caagcgcgaa aaaaatataa gtttccttct
    13561 attgatttgc tggagcctac gcagtcactt gaaaaattga ctccggaaga aatcggctac
    13621 cttaatacaa aagttgaaca gatcattgcc aagctggccg agtttggcat tgcaaccaaa
    13681 gtggtggcta cggaatacgg tggaccggtg gtagctattt atcaactgga attaccgtcg
    13741 ggattaaaaa tttccaaaat cacgggcttg gaagacgaac ttgcattgtc cctcaaagtc
    13801 aaatccgtac gtatggtacc ggttaccagc aaaggcacga tacaggtcga aataccgaaa
    13861 ctcaaagccg agcctgtgat gattcgcagt ctttttgaag atccgtcatt tcgcgatgcg
    13921 aaaaacaaat acaaactggg tttggcattg gggaaaacga tagacggtca tattcatttc
    13981 gaagatctcg ccaaaatgcc gcacttgctg atcgccggta cgaccggcgc cggtaaatcg
    14041 gtaggtatca attcgatcat caccagtctt ctgtatcagt tcgatccgtc ggaagtgaag
    14101 tttatcatgg tggatcccaa gaaggtcgaa ttggcgctat atcgcaggtt aaaaaatcat
    14161 catttgattt ccctgcgtaa tcatcgcggt gaagttattg aagatgtcat tacgaaaccg
    14221 gaaaacgcaa agcttattct caaagcgctt gtcgaagaaa tggaagagcg ttatgaaaaa
    14281 ttggcagagg ccaatgtacg taatttggaa gattataata agcgttggaa agaaggccgg
    14341 atgccgcacg atggcaaata tcagcattac aaattggaat acatcgttgc gatcatcgat
    14401 gagcttgccg atctcatgat gacggcaccg cgcgagatag aaacttccat aacgcgcctt
    14461 gcacagatgg cgcgtgctgt cggtattcac ctgattgtcg ccacacaacg tccttcggta
    14521 gatgttttga ccggcttgat caaagccaat tttccggcgc gcatagctta cgcggtccgc
    14581 tccaagatag attcgcgaac catcctggat atgggcggcg cggagcagtt actcggcaaa
    14641 ggcgacatgc tttttatgcc gcccgggcaa gtgccgatac gtattcaaaa cgcgtttact
    14701 tcgacgcagg aaacagagac catcgttgat tttataaacc gcatgcaggc atttccgcgt
    14761 cgtgatttta tggtgaaaga agaaccgcgt gaggagaacg aaggcggtga ggtggattcg
    14821 tcgggatttg atgaactctt taatcaggcg atggagatcg tcgtcaaaca taatcaaggt
    14881 tcagcttcgc ttttacaacg ccgcctatct gtcggttatg ctcgcgcggc aaggcttatc
    14941 gatcaactgg aacgtgcggg tatcgtaagc gctcccgacg gggccaaacc acgccaggtg
    15001 cttattacgg aagatcagat ggcaatgtat atgaattagt gttcgtttga ttatgtttat
    15061 tgtataacca ttttccaaag aatggagttt atcgtttgaa gtatttgatc gtaagtttga
    15121 tggctctttc cggtgtttgg gctcaaggta aaccggaaga cgcacagtcg gtcatccgta
    15181 aagtgcaaaa caagtataaa aaaacggaca gtttttcgtt ttcgtttgat taccgtttca
    15241 aatggaaact gaccggaaaa agtcagagcg ctcagggcaa attgttcttc aaaaaggaaa
    15301 acaatattcg ctatgagctg ggtcaacaac tcaatatcac caacggggaa tcggtgtggc
    15361 aatattcgga agtcaataat caggtgatta ttgataaact gaaaaagaac agccgcagcc
    15421 tgtttatgcc caaatatttt ttgtatgatt atttggaaaa atttattgcc gaagtcatta
    15481 aaaccgaaac gatcaacggc cgattagttt atgtgctcaa acttgatccg cgggaccgtg
    15541 atgattttgt gcaaagcatg aagatatggg tgccatccga tacgtggatc acccaaaaaa
    15601 tagaatacgc cgatctggag ggtaatcaag ttacatacga atttacccac gtcgaaatag
    15661 atcagccgat gaatatgaat ctttttacat tcacgccgga aaccggtatg gatgtgattg
    15721 atttgcgatg accggagtgt aacttgaaaa aagtactatt tcttctgctt aatgttttgg
    15781 ttagtgtttt ttttgtatgg cttgcgacac gcggtataga ttggacacaa ctgggttcct
    15841 cattaacttc gatcagtctt acgcttgtga tcttttcagt aatcatcaat caatttagct
    15901 tatggcttcg tgcctggcga tggaagtttt tattggctcc cttaaaagac gtgccgacgc
    15961 ttagtctgtt ttcaaatgtc agtatcggat ttatgatcaa taacattttg ccgttccgcc
    16021 ttggagaaat agcgcgaggt tatattctaa aaaaagaaca tcagatttcc tttacggcct
    16081 cgatggcaac ggtggtggtg gaacgtgtca ttgatgtgat ttcgttgctg acggtttttg
    16141 gcgtacttgt aattattttt ccttttccgg aatggatcaa aacggccggc atggttgtcg
    16201 gcgcgataga tttagcggct attattttca tgatcctgct tgtaaaaaat acggaagcca
    16261 catgccgctt ggtacaatcc atgatttcag tttttccgga tagtctcaag caacgcgtag
    16321 atcgtctttt acgatctttt gtgtcgggaa ttacatttac gcatgctttg ggtacaaccg
    16381 tagtcattgc tatgctcagt tttttgatct gggctgttta taccgtatca atatggataa
    16441 tgtttaaagc atgcaatgcc cctgagttgg cgacactgga tcttatagat gcgactattg
    16501 tgatgacttt ttcttcgttt gccgtgatga ttccggcggc gccgggttat gtgggcacat
    16561 ttcatgaagt cgtcaagcag agccttatga tgtttcgtgt tgatcgggag gttgcgcttg
    16621 gatttgccat cgtcatacat gctttcaatt atgccgcgca gatagttatt ggtgtcggat
    16681 attggattaa aactaatttt agtctcaaag aggcgttatc gaatgtacat gaagaaaaat
    16741 aattttttga aaatgcttat catcggattg ctgatggtaa tgtccatgag cagtagtgcg
    16801 ggtgagcttc acgcgcaaac aaaagattcg ctcaaaacgg atgctttatc gcaaatacgt
    16861 gctgatcgca aaatcagacc gggtgatatt ctggatgtcg tggtctacgg ccatgacgaa
    16921 ttacctaaac aaatcaaagt cgaagccgat ggtaccatca aatatccttt tatgaaggat
    16981 atcaaagtgg acggtatgag cgtcgaacaa ttgacgaata tcttatcggc ccggctctcg
    17041 caatatatcg gcggtcgcgc cgaagtgatc gtggcctatg ctaaagatga aatgatcgaa
    17101 gtgattgttc tcggctctgt tgctcagccg ggttcgcaca aaattccccg taaccacact
    17161 attcaaggtg ctattgcgac ggcgggcggc gcgactgtgc gcagtgattt agatcgaacg
    17221 aaacttatcc ggatcaatcc cgaaacaagt ttgcgcgaag agatcgaagt gcccatggtt
    17281 tccatcatag tagaaactgg cgatatcaga aaactgcctg ctttgcgtga cggtgatgtt
    17341 atttttgttc cgtctattta tggtgcagta tatgctaatg tactcggtgc tgtgggaaaa
    17401 cccggtaatt acgaattatt tcccggtgcg aatctggtgg atgtgatttt tttggcgggc
    17461 ggtcccaaag acgatgcttc gattcgcact gtcaaattga tacgtcgtat cggcgtcgca
    17521 caaacggaag aagtcgttga tgtagaggcg gttctcaagg ccaaaggtgg ttcggttccg
    17581 ctcgtgcaac ccggcgatat tgtatttgtg ccggcgaaaa agtttacgct gaaaacgata
    17641 tttcaagtgt tgagttatgc ggcgacgatc ttaaccttga ttttcctttc caaacaagct
    17701 ggtttattgt aataccacat agaaagtaca ggacatctat gagtgactta aacgatgata
    17761 agcaagggat tgacgcgcgc gcgatcctgc aaactgtatt gatgcacaag tggattgtac
    17821 ttacggtttt catcgtgttt tccggtatag tatcgtatta ctcgttgagt aaaccgacct
    17881 tatatcgctc ttcggctctt ttattttttg ataacggatt gtccaatctg gcttttgcta
    17941 atgttcccca acagcaaaaa attgccttag gatcgcagga tttcggttat tatgaagtca
    18001 tcataacaac cgatctcttt aatcagcggt ttcgtaagga gctcgaacgt gtcgtattga
    18061 gcgaacgtaa tgatgcggaa actgttttgg cgctcaaaaa cgtatcatca actcaggtga
    18121 cattgaaacc atataaagaa gtagaacagt ttatcgaaat atcggcggtg aattatgact
    18181 cgtttttggt taaaaaaatg attgaagtga caacggacgt tttgaagtcg cgcgtgaccg
    18241 aactggatcg ggaagggctt cagcggagta tcgagtttat tgacgaacaa gtggaagtaa
    18301 gtagaaataa tattgaaaaa acagaagtgg agcttcaggc gttacaaaaa aagacagatg
    18361 ttaacatcaa acaatcggaa ggtccgctgg ctaaagtact gatcatgaag gaaaaattag
    18421 ctgaatttga aacgcaaata caaatccgaa gctccaatat taacgcactt aattcacaat
    18481 tggattcgat tcagcgacgc ctgacagggt cgtcttctta tgcggctaac gaatccacgg
    18541 aagaaaaaca aatcaagtta cagattgaac agttgacgtc gcaaaaggcg gttttatatg
    18601 aaaagcttgg ttcggatgcg gtagaaaatc ccgaggtaca gcggatagat cgtcaaattg
    18661 ctgacctgcg tagtcagtat tacacgctgc tgtcgtccgt taaaaccggt tcagataata
    18721 cggtcagcgg tgatatgagt caggtgtgga agacgatatg ggacaaaaaa aacacggaag
    18781 aaattgaact ttttttactc aaaggtcagg cgcgtttgtt tagcgggctt ataacaaact
    18841 ttgaaagaaa aaaccctaac ctgctccaag atgcaattga tatttcacgt ttattacgct
    18901 tgaaacaggt ttatgaagaa cgcctgagtt cgctgataaa acaaaaagaa agtttgtcga
    18961 ttcagtatta cggcagtaca gggagcctga aaattatcga tcccgccaag gaaccggtag
    19021 caatatataa taaggtattt accaatattt tcgtcggaac cgtgctgggt ttaatcatag
    19081 gtattgccat tgcggtaggc ttagaattta tggataatcg aatcaaatcg attgccgcga
    19141 tcagttcggt tacttcgatc gctgttgtcg gacgtatacc tccgatcaat gtaggcgata
    19201 aagataatgc cgtcgagggt ttgcggaaaa aaatcaataa aatcaaaacc aaagatgagg
    19261 aagaagataa gaatgaaatt cgtcggaaag cgatgatttc acagtttaat ccgcgttcgt
    19321 ttgtatcgga gcgctaccgt tcattgcgta caaatattca gtttgccaat attgattcgc
    19381 cgattcgctc gttgcttgtg gcgagtgcgg gtcctggaga aggtaaaaca accacggcgg
    19441 tcaatttggc tatttcgttt gcggatatgg ggcatcgcgt ttgtatagtg gatacggatc
    19501 tacgtaaggc caagcatcat atgttatttg atatacagga gcaaccgggt cttaccgata
    19561 ttattttagg ccaaacggat ttagatacgg cgcttcaaaa aacttcgatt aacaaccttc
    19621 attcgcttac cgtcggtaga gacgctaaag atcattcgga aattttctcc agcttgcgta
    19681 tgaattttat gatgaatgaa ttggagaaga agtttgatat cgtcgtatac gataccgcgc
    19741 ctgtaatgtt gttgacggat tcggttattc tggcttctaa agttgacgcg gtgcttatgg
    19801 tggtcaaaca tgccgtaacg ccgaagtcgc aactccaaaa tgccattcac aatttaaaag
    19861 gtgttcgtgc caacatcatc ggtatcgtga tgaatgatta cgcagatgag cggaagaatt
    19921 attataaata tgcatacgac gggtattatc tttccaagtc acaacgtatc agcacgagcg
    19981 aagaaggtgt aaaagcatga gtaaaatatt agtgaccgga tgtgcgggtt ttatcggttc
    20041 gcatgttacg gatgcacttt tgaaagcggg ccacgaagtc gtcggtttgg ataattttga
    20101 tcatttttat gatcctgcga ttaaagaaaa aaaccttact aacgctattc aaagtcccgg
    20161 ttttcggttg ttacgtggtg atattctaga cgttgatttg atgggtcgta ttttttccga
    20221 atcgaagttt gatttggtaa ttcatttggc ggcacgtgcc ggggtgcgtc cctccatcca
    20281 agatcccaaa atttatcaga aggtcaatgt tgagggtacg atgaatattt tggaagcgat
    20341 gcgagcccac aatgtaaaga aactcctact cgcgtcctct tcatccgtgt atggtaataa
    20401 tgaaaaagtt ccttaccatg agacagacaa tgtagatcat gccatatcac catatgccgc
    20461 gaccaaaaag gcatgtgaag ttttggctta tacctatcat catttatttg ggatcgatgc
    20521 gttttgctta cgatttttta cggtgtatgg ccctcgccaa aggcctgaga tggcgattca
    20581 tttgtttacc cgtttgatag atgaaggcaa accgatcact ttatttggtg atggacagac
    20641 gtttcgcgat tatacgtatg tggatgatat tgttcatggg ataatgcaat gcattgacgg
    20701 tcttcgcgga tatgaaatat taaatctcgg cgaatcgcaa acgatttccc tcatagacct
    20761 ggtcaatacg atagaaaaag cattaggaaa aaaagctcaa attcggtgga tgcccaatca
    20821 gccgggggat gtacagaaaa cttatgcgga tataagtaaa gcgcggcacc tcttaggtta
    20881 taaaccttcg gtcggtatag aagaaggggt gcggcggttt gttgattatt ttcggtcgac
    20941 atagcgattt attgtttatt ttcttcattt aaatttcctt cgctattggt gaatccttaa
    21001 gttgaggttc tttggacaac gcacggcaaa aagatataac taaattagcc aaaaaatttg
    21061 caatatcggg tataggaagt atttttggtt tattttggac accacttaca agtgtcatta
    21121 caactcgttt tctaggagct gagttgtatg gaatattttc tctggtgcag tcgtggggtt
    21181 ctttgcttgc caatttgtcc agcattgggt taaacggaat gaatctacgg tttataccca
    21241 cctacaaagc aacaggttca atggcgttga tcaaaggttc agtatttaca actattcgcg
    21301 tatctgtcgt gataagcgca gcggcaacgt gttttattct attcttccct gatttgtttt
    21361 gtgaattatt tatccacagg cctgagacta tttcggaaga atatttctca aattatgtaa
    21421 caaaagcatt tcaattttac gctgtctcga tattacttac ggcatcatat caggcgtttc
    21481 tatcatcact agtgggatat caatttgttc agtacaaggt tttggcgaat gatgtgcttg
    21541 gtccggcggc aaaaattata agtcttacct cgttattgtt attggatttt aacctctatg
    21601 cagcgcttgg ttcgaatatt atccaagata ttgtcgtact tttggtgtca gcattttatc
    21661 tttttcgaat agctcctgag ctcagggata aatcaatacc cgttcaaata gaaaagaaga
    21721 aaatgatgaa attttcttcg gcactttttg ctaattcgct tttaagtaaa tacacctacc
    21781 aacttgatat tctgtttttg ggatattacg ctactttgcc ggagattggg atatacacat
    21841 tggcactacg cttgcaacca ttaatctatc tacctcatta ttcgatttcg tcagtttttg
    21901 ggccaatggt ggcagagtta tatactgcgg gtaaaataaa agagcttaac caattttata
    21961 aaacaactac aaagtggagt gtaacgctca gcttaccaat atgctcgtta atcatacttt
    22021 ttagtaaaga aattcttggg gtttttggta aagaatttgc tgccggtttt gagttagtag
    22081 ctattctaag tatcggaaat ttaattcatg attttttagg cctttcagga aatatcatac
    22141 ttatgactgg gcgtattcgc atcaatgtga ttaactccat agtaatggcg atcatcaact
    22201 tgcttcttat gtccatttta attcggaatt atgggatgta tggtgcggcg atcggaaatt
    22261 ttttatcaat aacgatattg tcaatactca cgactgttga aacgttttat atactaaaaa
    22321 tacatccgtt tgatttttta ttgttaaaac ctttttttgc aatgttaatt tcgtcttcta
    22381 tcgttatcgc tatttcgatc tggtatcgtt taccttatta cgaaattact tttttaatct
    22441 atatacttgt tttcatttta ctgtatgttg gatttttatt tctttttggg ctatctcaag
    22501 atgatcgaat gatcttggac aaaattctaa aaaaacttcc ttttaaaaag cagaacagca
    22561 atgaatgata aacatcatta tgatggtatt tcggtagatc aatacgataa tcggaaaaat
    22621 cgtaatgtat ttgaaaaata cttatatgat cattggcacc ccattatcgt agaacatatt
    22681 cgtttgttgt gtgacaaacg agatgtgcta gatttgggat gtggttctgg agcttatata
    22741 ggttttgctt tggagtctac ttgcaaggtt tcaggtttag aggtttcaca aaatatgttg
    22801 aatgccgcga aaaaacgtta tcccaaagcg gattttatcc tcggagatgc tcaagaaaca
    22861 agagtagaaa gcgaatcatt tgagataatt tattcgatcg gattgttaga atatgtaaat
    22921 gtggaaaata cgctcaaaga atgctatcgt attttaaagt caaatgggcg tttaatgatt
    22981 ctaacgccaa ataaatatgg tgggagaaaa tttgttacgc ggtatctgaa agggaaaaaa
    23041 aaatcctctc caaaatatta ttcgagacga gaagttttaa acgctcttga taagcaagga
    23101 ttttatattg ataaagttat aatggatgat ggattgattt accttcctaa tattactccg
    23161 gatattattt cgcgtttgat atttcgcaca aatgaaactt ttttcaaatt attcccatat
    23221 aacccattca gtcaaaacat gatttttata gcgaccaaac gatgatgggc aatgacgccg
    23281 aaataaatga atattcgctt caaaacttgc gcaagtattt tcttgtctcc atacaaaatt
    23341 atttagagat tttgatgtta atgcttttag ttccaatagt atggcttatc agtaatcctt
    23401 atgattttat agcctccaat gatccctggg agtattttaa tatttctcac ggtttaatca
    23461 ttgggaatgg gatcgtgttg aacgacaact atgatgttat tttaccgcca gggtatcccg
    23521 ttattatagg ccttctgtat tttttgattc ctgtagaatg gcctatactg cttggtatcg
    23581 gcattgtttc atttatttta tccataatct tgatgaaaaa aatattacaa ttaatgggtt
    23641 ttaattttca tctccgtatt atcggtttgt ttttttttgc atttaattcc caactattaa
    23701 ttcaggccgg aagaggttat tcagatttgc ttatgatctt gctgatctta tcgcttatat
    23761 gggtatcaat tatcaaaaag aatacaaaga atcttagctg gggaattgtt ttaggaatgt
    23821 tatgggggtt attgtcgcta actaagccgg aaggagtttt gctggggcta gttataattt
    23881 cattgagcgt ttttaatagt cgtatgatca aagggatcgc tgcaatctgt tttatcgtac
    23941 tctatctacc gtatggtgtt tttcttaaaa atcagaccgg cgcctggcaa ataagcggta
    24001 aaacatacgt caatcttgtc cttggcgaac tgaatagccc atatcaaaat cctacgaatt
    24061 atgatactcg atatgccgtc ataagtcgag tatgggaaaa cgctgaagag gcggggggga
    24121 cttcggtgta tttgattaac cttggctaca aaatacttga acgcgtacca tataatataa
    24181 aacgtttttc taactatttt gtttctactt attcgtggat aggaggttgt ttcttattag
    24241 catgtgtaat tttttggaat tcaatctatc gaagattcat tgtgttgagc attggtttta
    24301 catgttttta cttatttttc tttattatac ctagagttct tactttatat catgtttttt
    24361 tttcttttgc tatgctggct ggttttcaaa ttatcttaac gcatataaat cgtgtgagaa
    24421 atcgttggct gacactttct ttttacggcg ctgttgtatt ttttattagt tttcagtttc
    24481 gaattgtttt aaaaatttta atgcaaaaaa tataacatct aacttacaaa gtcgcccgtt
    24541 ttttgaaatg atcacatgag aaggacagtt ttttttatag tatcaattga taccgagtgt
    24601 gataaaagta ttgattggag tattacgtat ccattctcgt ttcgtaatat ccatgaaggc
    24661 gtagctgaaa aacttcagcc actatttgat atatataaag taaaaccaac ttatctattg
    24721 agccctgagg taatctataa tcaagaaagt gtgaactatt ttaaatcaat tcaagataga
    24781 tgtgaattag gtacgcattt gcatggtgaa ctcataggct atgataaact tgaaaatcca
    24841 agatggacta acgattttca atgtgactat gatgaaggta tagagcggga aaagcttgaa
    24901 tcccttacgg aattatttaa aaataaattc ggctatatgc cgaaatcttt ccgcgctgga
    24961 aggtttggca tcggaccaaa cacatggcgt attttggccg agcttggtta tgctgttgat
    25021 tcgagtttac ttccatttca attaattaaa acaaagaatg ctacgtacaa tttttactat
    25081 actccggtta aaccttttta tcctgatttg caaaattgga tgaattttga aaaacaagag
    25141 attcgtaaaa tattgcatgt accaatgacc gttcataatt ggttttttca acgcattccg
    25201 ccctgtattg gaaaacctct gtcggagcaa aaccgaataa gaagcctttt tcgtattctt
    25261 cttacaaaag aaaaggaaaa aacgtattca ttaagaccga gaacagacaa tgctgagagc
    25321 ttgatcaatc tgattaaaca tcatataaaa attcatacta ctaaggaacc tatagtattg
    25381 aatatgatgt tccacagtaa tgaactacat cccggcgcta gcccatatgc acaatcacca
    25441 ttagaggtag ggcaaacaat aaacacaata aagcgcattt tagattacat aaatcgttat
    25501 tattctatcc attccatcgg tttatgcgat tctactcggt attacgaaag agatacatga
    25561 aaattcttca tatcataagc aatgggaata tcggcggacg agagaaagtt ctttatttac
    25621 tattaaatga agaatcaaaa gataaacaaa ttcagacgtc ggcttttttc aaatatcccg
    25681 ttggtcctta ttatacaaaa ataaaatcgc tggggatttc tgtttattat aacataactc
    25741 gcttacgaga tattatttct ttaatggaaa agcaggacat aatagttttc cactttgtag
    25801 attggagatt cttcttggcg ggtattttat cagggaaatc tattttatat cgtttatcag
    25861 gaatttattt acttaacaag aaaagtttgg gcgatgtatt cttcatttta gcgaaaaaaa
    25921 taaaaaaaac aatatggaga cgacatagcg aggatgaccg taggggcgat caattaataa
    25981 aattaccggc aaaggagaat acttcccaat caaaaattcc taacagacgt tcattataca
    26041 gacttattaa acgaaaatat ttcgagctat ttctccgcaa gtggtgtact tatttaatcg
    26101 ctaatagccg ctataccgct aaaagtgctg ttgagaaata tggcattcaa ccccagaaaa
    26161 tacgcatagt aatgaatggt atcgaattca ataatagaaa aggcgatgaa acagattttc
    26221 gtgcatcata caatatagat aaaaatgttt ttttgatagg aactgtatgt cggtttgaca
    26281 ctcgcaaacg cttagatagg cttctcatgg gattttccat gatttcgaat ttaaaagatt
    26341 tccacttagt tgtcgttggc ggcggtgata cagttataga acaagccctc aaacaatttg
    26401 tcgcgcacaa taacctgcta gataaagtat cattcacagg tattctggaa aattcatctg
    26461 atgcaatgtc ggcgatgaat ctgtttgtat tatcctctga taacgaaacg tttggtgtat
    26521 cattggttga ggctatgtat aaaaagaaac ctgtagttgt tttttccgat agtggcggcc
    26581 ctgcagagat tatatcaggt caaaactgcg gttatattat taatactcca tctgaaatgc
    26641 cgggaattgt tgaacaatta cgagctgatc cgattttgac acagcgattg ggtgagaacg
    26701 gtcaccgtct agtggttgaa aagtacaatg ttaaacggtt ttataagaac tcattgagag
    26761 tttattcgga acttttcgaa taataaaatt tgaggttaca tttatgtgtg gtattgcagg
    26821 tgttatgaat tttactggtg agcccgttaa tgaatcttta cttaaggcga tgggtgacgc
    26881 cttggctcat cgtggccctg atggcgatgg tttttttatt cgtcaacatg tcggattagc
    26941 gcatcgacgt ttaagtatca ttgatctaaa aacaggtcaa caaccgatgt atgatgacga
    27001 cggcaagtct tgcatagtat ataacggtga aacatataat ttcggcgaag ttagggagga
    27061 actaataaaa ttaggttgga actttcgtac ccaaagcgac acagaagtta ttcttaaagc
    27121 ttatctacag tggggggcaa gttgcttaga cagattccgt ggaatgttcg cattagccat
    27181 ttgggatgaa cgtaccaaat cgcttttttt ggcaagagat cgagttggaa ttaaacccgt
    27241 ttattattac gcagatcaga ataagttttt atttggttca gagataaaag ctattcttca
    27301 ggataaaagt atagatcgaa ctattgacta ttcagcgatg cgtgaatacc tgcaatatgg
    27361 ttatgtacct tctcctgctt ctatttataa taaaataaag aaactccctc cggctcactg
    27421 gcttcatatt acgtttgatg ggggcaggta taatatgaaa agcaaatgct actgggacat
    27481 tcagtttggt caagatacag atggagcaca caatgataca acgtggatcg ataaaactga
    27541 atccgttgtt tcagattctg tccgcataca tatgatcagc gatgtgcctg tcggagcttt
    27601 tttaagcggg ggtattgatt ccactatagt gtctcatttt atggcgaaac acgttggcac
    27661 tatcaatacg tttacggtca gtatgaacga aagcaggttt gacgaaagcg catacgctcg
    27721 tcaggtgtct gctatcatac aaagccgaca tgttgaaaag agtgtgcaag cggcagatct
    27781 caatacgatt ttatcgaatt tagtatatgc ttacgatgag ccttttgcag acagctccgc
    27841 tgtaccaaca tatcaaatcg caaaaataat tcgatctcat gtgaccgtag ctttatcggg
    27901 cgatggtggt gacgaagtat ttggcggtta taaacgatac gaacaacttt tggaatggtc
    27961 ccaatcaaat ccatggttaa ataaagtttg gttatcaaaa atagccacat tttggccgcg
    28021 aaatatacct ggaaaaaatt atcttcgata ccattcgcaa tcagatatga ttcgttatta
    28081 cggtatgatt aatatttttc attcggacca aatcgcagat atgatgaaat tctctgtaga
    28141 acatcgggaa cgattattcg actcttatat gaatagctcc gatgcgcttt tgaataaaat
    28201 gcaaaatata gatatacata cctatttacc tgaaaatggt ctcacaaaag tggatagagc
    28261 cagcatggca cattctattg aagtcagagt tcctctgctt gatcacaagg ttatagaact
    28321 tgcggcgcaa atgccggttt cgatgagaat gcgtaaaagt gaaaaaaaat atgtactaaa
    28381 aaaaatattg gagcctcgat tccctaaaga actaatttat cgaaccaaac gcggcttttc
    28441 attgcctatg cgtgactggt tcaaatctaa tcccgattta acccgtatga ttcatcacga
    28501 aataaaagag tgtgagctga tcaaagaaca catgaacctt cattttatag aggccatgat
    28561 tacgacccat ttaaacagtt ccaaaaacca tgcttcacgc atttggagtc tttggttttt
    28621 ggcgcgttgg tatgatacat attataagaa aatgtaacct atggcttttt tataataaat
    28681 ggaaaaaatt ctccttagta tttccgccgg tatcggtgat gcgctgatgg cgcagccgtt
    28741 gttgtgcgaa attcaaaata actttccgca gtctcacata gaggttctct gtagcgccgc
    28801 tacacagcat gtgtttcgtt cgcatccggg tattcaaaaa atctggacga tggatgtttc
    28861 gtcgataaaa aaatatttcg ggttggttgg gaaaatacgc aaagaaaagt ttgatctgta
    28921 tatcggcgct attccgtcga acacctactc gcaactttta cttcctgttt tagcgggtat
    28981 accggttcga atcaaacata actcaccgca taaatttcct agaaacttag atgccttgta
    29041 tcatgcgctt accccgatcg gtgaaggcgt tcatcggttg gaatgcaatc ttgaactgct
    29101 tcatttttta aaagttaatc actggtcaaa acaatcgcca aaatttcata tcagcgatgt
    29161 tacgctagcc aaaatggaaa caagaatagc gcatgacagg aaaaaactta tcattggttt
    29221 tcatcccgga tgcaaccctg ctgcagcttt caaacgttgg ccgtcggaac gttatgccgc
    29281 tttggcggat tatttatata ctacatacga tgcaactgtt tttatcgtcg gcggtaaaga
    29341 tgaagcggat gatgtgcgaa aaattatgga gcaggcacag cataagccgc tcaattttgc
    29401 aggacagtgt actttggaag agaccgcggc gttgatcgcg caagcgcatt tttttgtttc
    29461 taacgattcc ggaattatgc atctttcgac cgccgtagga acaccggtgt ttgcaatttt
    29521 tggacccaaa gatgagcggc atatcggacc ttacggtgaa aaacataccg tgattcgtaa
    29581 cggccgcgat gttaatcaag tgacgctcga acaagtcgta caaacattga tcgcttcgaa
    29641 attcggatta aagccattta tcaaatcgta agtatatgga acccatgatc gaaatacgcg
    29701 gtgcacgcgt acacaatctc aaaaatatca gcctcaatat tccacgaaac aaattcgtcg
    29761 tgatcaccgg cgtcagcggt tccggcaaat cttcattggc gtttgatacg ctgtatgcgg
    29821 aaggccaacg gcggtatgtc gaatcactat cggcgtatgc gcgccaattt ctggagcgca
    29881 tggacaaacc ggaagtggat catatcaaag gtatcagtcc cgcgttggcc atcgaacagc
    29941 gcacgttggc caaaaatccg cgctcaacgg tggggacggt tacggaaatt tatgattttc
    30001 tccgtttgct ctttggtcgt gcaggaaaaa catactgccg aaattgcggt gcattggttc
    30061 gaaaagatac cgtcggttat gtcgtacaac ggattctcgc tttgccggag cgaactaaat
    30121 tgcatatact ttttgaactg gcagctaacg aacaaacgat tttgtcgctt cacgaaaagg
    30181 gctttacgcg cttgatcgtg gatgatgtta tcgctgaact caatgacgaa gccgtcgtaa
    30241 aaaaaatcct gaaacaaaaa atagttcgtg tactcgtaga tcgtatcgct gtaaacaaag
    30301 aagaaaaagc gcgtgttgcc gattcggtcg aaacagcttt cctcaacggt gaaggcaaaa
    30361 tgatcgttca aataatggaa ggcgaaacgc ttcgtttctc caataagttt gaatgcaatg
    30421 cgtgcggcac ggcatatatc gaaccagatc cgcgtttatt ctcgttcaat aatccttacg
    30481 gcgcttgccc gtcatgtcag ggttttggta ataagatcga agtagatatg gatcttgtca
    30541 taccggatcg aaccaaaaca cttaaggaag gtgcgatcaa accgtggacg acggacggtt
    30601 acgccgattt tcaggaacga ttggaaaaag cggctaagaa aaacggcatt ccgctgaaca
    30661 ttccggtcat ggatcttacc aaagagcaat acgaaaaagt catgcaaggc ggagacggat
    30721 ttgacggaat tcgtgaatat tttaaatggc tcgaaagcaa aacttacaaa atgcacgtcc
    30781 gcgttttgct cagcaaatac cgtggttatg t
//
LOCUS       DAOQGF010000032        30298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_676_length_30298_cov_6.463115, whole genome shotgun sequence.
ACCESSION   DAOQGF010000032 DAOQGF010000000
VERSION     DAOQGF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_676_length_30298_cov_6.463115"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PK937_06465"
     CDS             <1..1131
                     /locus_tag="PK937_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32248.1"
                     /translation="RSFFAQASVSGKVILGSTKQPVKADWVTLIKSGQGLQTLQQLDN
                     VSEYAFKDIPLNPMAPYLIRASYKGILYTQNVMINENKPYIADVSVAGVSDQWRDIDV
                     RVPHMIVARTDDKLEIEQTFEITNNGANTFNPKDTNVPSFLFKLPDGVKLEAVTTSFD
                     KSMPIPASVVNYKDKQGIIFPLRPGTTQIQITASAPYASPSLTWAQPMYYDVSEFRIF
                     VTPKDIEVSGENFQHIHDEQLEANNFSITAATNLKSGSTIGISFKGGSARGGAPQEDD
                     HPIDAAANEIQRNVFFLAPLLMSFLLFALYIALNRKHQSDGGGAQQKAHWMAEQEKLI
                     ASIARLDDEYAENSDNSEYVDRRRDLKHQLRDVSKRIQRAGA"
     gene            1128..1811
                     /gene="ccmA"
                     /locus_tag="PK937_06470"
     CDS             1128..1811
                     /gene="ccmA"
                     /locus_tag="PK937_06470"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNH32249.1"
                     /translation="MIRLQHISKQFGRFKALDDLSIEIPSGDFMALLGPNGAGKTTTL
                     KLLAGLIRPSSGAIQVDGMEKNSRSVFLQSIGMVSHQSFLYNDLTAIENLRFYANMYT
                     VSNASARIASLLKRVGLGARGDDIVRTFSRGMLQRLSIARALLHEPQMLLLDEPYTGL
                     DVHGCRFLDEVLKEYHAGGHSIVMITHDLDRILGLSNRIVVLDNGRAVFDRRSVEPEF
                     STFKNYIRN"
     gene            1816..2871
                     /locus_tag="PK937_06475"
     CDS             1816..2871
                     /locus_tag="PK937_06475"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNH32250.1"
                     /translation="MNQESLRVIDKSNYYDVLCHFDQHITDALDRFDGQKSNLSGLSK
                     SRAGFRNILICGMGGSAIAGDFIKNVWTEVLGLPIQVCRDYHIPRWVNKESLVIACSY
                     SGNTEETIAALHEAMEAQAQILTIATGGTVQSIANQHGLSHITIPGGLQPRQAIGYAL
                     TTLHAVFSEIFKSAPVREEMSHIVFQMKHLATILKTVHDNNPYIHDSKILHDKPVIIY
                     ASERMFPAALRLKGQITENAKLLAFAYAVPEMNHNEIVGWESVHEAHHAPCHVILLRD
                     REDHPQVQKRFEILMRLLKGKAHWTEYTSDGGTLMERYYNLIYRGDWLSYYMAIARNT
                     DPTPVDIITRLKNELAS"
     gene            complement(2883..6422)
                     /locus_tag="PK937_06480"
     CDS             complement(2883..6422)
                     /locus_tag="PK937_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32251.1"
                     /translation="MLYRFAVIFLSAIVIDLSVASAQDETPPYISADSSIQHTLLSKN
                     INYFQGAHTIRGYSSDTLYYLPFRRIIPESEIFTIGYQKLTREKDYTIRYSDGVVRFS
                     FVVDSSITVECRFLVSRLGIDDSYARRDLPIIRTNIISSKTDTIPRWTPPNPSSEFTN
                     DLNGRGTITRGFTIGNNQDFTLNSGLNVQVTGQIGEDVTLEAVITDEQTPIQPEGNTE
                     RLQEIDKIYIEVRKADKFTATLGDFEIQTTGTQFGNFHRSLQGVRSLYNDGRTDAAAA
                     YGTAKGKYHSNTLTITEGVQGPYELTGKNGERNPIIVAGSERVWLNGVALTRGENNDY
                     VIDYNAGQITFTRKRLVTPESRIIVDFEYSNEVFRRNTASASVRSQFFDRRLTVGGAF
                     LSESDDKNNPLNLSIDQQLRDSLASLDDRGLQGNGNTILVDGGRWVGEGRGAYYKVYD
                     ANSNDSIYVYSGSDSSGSYNVRFTDVGSGRGDYIRGNILGEFIYVGSGLGYYLPLVPM
                     DLPTRLRTGIWYAAWNPVQNLRLRTEFALSQFDKNLYSPRQSQGYAYQLEAFVADQDI
                     RLFKTKLGKFDLQIIRRNQDSNFTAPDRTQDAEYGRQWNIFNPATSVYSVTAPGLQET
                     VNDFRLAYKPWKGVQTDFFKGELTRGDHQFVSTKSGSGLKIDRKNIFVSGSIHDIQIT
                     DRTQSVIVNNHTQRKFFDFNGAWRIWRPGLYYEDESQKNRQSDSVYGASYQVIRPNLN
                     IITGQYLTLGASYEMRSDRIRNDAVDSLDGRVSKSINQRYYWQWTEWHNLNHTFEWQR
                     RKREYFGSKKTIANPDRISNLVNASADYHPFSRAVQVTVDYQISQEQLQNRKIVFIPV
                     PPNTGNFVQVRQDSFRQVPQGQGDWIQSAVRGQGFTPVVELKSGIRLRLEIGRLRSRS
                     SKDSISDRWQSRFIERLIYEGLFRIEENQTDPDRRFYWLQAVQNKNTQRGNLLLRQEL
                     SGYDRIYDMLWRLRYETNRTRNRLLNDGQDKNQRDIYYLRIQKQVNSFWSWEQEGEVT
                     IAQRQSLINVSSLNIRYDIHKNRYSPSIVFKPKPTMEYHLRTDWLLAKDYETGSRAKI
                     MNWQPTMVFSWLNRGRWTNTLELTRLWLKDGLGNTPFEVTDGRVRGWNWLWISAVDYR
                     AGEHVIMRANYTGRKYPGQKIIHLGTAEVQAFF"
     gene            complement(6423..7343)
                     /locus_tag="PK937_06485"
     CDS             complement(6423..7343)
                     /locus_tag="PK937_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNH32252.1"
                     /translation="MKFKTICIFLMICCVPLLGQEEIRFQIKQVIPIKTPDGNNVTQI
                     NSVCVTPLREVYMTESSGHRVLRMNTEGQMLGLFGGLGWSGEMLDYPTGITSHDGLNL
                     YVADMNNQRIVRLNRRLEWVASIGGHNLDYSGQESVHSVAYPLSLTQSKQGDLFYISQ
                     ATNRVYKVNSVTGTITEWNSFTAGGGYLDDPKQIVIGKKNLFIKDRTGVKIFDIFGNY
                     IRTINDTFFSANLLLSADEQDRVLIWDDDKKSLTIWNAEGQRVTLTIAFAFENPTAMT
                     CFGHSLWIVDQSSTVGESKLYQIEITEESR"
     gene            7396..8139
                     /locus_tag="PK937_06490"
     CDS             7396..8139
                     /locus_tag="PK937_06490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH32253.1"
                     /translation="MLFRNFISLSKNISMLLLGLTLANTACTQSAKTTEKKKVNAEVA
                     VIETDFGNIIIKFYDKEAPKHTENFKKLARAGFYDTLTFHRVIPGFMIQAGDPNSKDN
                     DPYNDGRGGPGYTVPAEFNKKRFHKRGALAAARQADQVNPRKESSGSQFYIVQNGPVS
                     LDVLKQMEAQFKAAKPGFYFTNEQIDTYTTLGGTPFLDGEYTVYGEVVSGLDVIDKIA
                     MVQTDPRNNRPVQNVIMKKVSIQTMEIEE"
     gene            complement(8217..9152)
                     /gene="miaA"
                     /locus_tag="PK937_06495"
     CDS             complement(8217..9152)
                     /gene="miaA"
                     /locus_tag="PK937_06495"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNH32254.1"
                     /translation="MNDLNPNVLVIVGPTAIGKTALAVDLSEYFDIEIVSADSRQLYR
                     YMDICTAKPDNTQRLRVPHHFIDHINPDETYNAAQYAREALPVIQSIFDRGKQPVIVG
                     GSGLYIRALIDGFFEGVDNNEIVRAEIRKQIDSEGIVSVFEELRQNDPETANRLHISD
                     VQRIERALEVFRVTGKPLSQWHKETQSKFPWNSLWVGLDCDRTKLYQRIEMRVDQMIE
                     QGVIGETEMLLERGYSENLISMQSLGYREIIAYLKGTMDCDTMVRLFKQGSRNYAKRQ
                     LTWFRKNERIRWIMTDKNSPEKILDNTLKHFNLNK"
     gene            complement(9169..10980)
                     /gene="mutL"
                     /locus_tag="PK937_06500"
     CDS             complement(9169..10980)
                     /gene="mutL"
                     /locus_tag="PK937_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNH32255.1"
                     /translation="MENRIQILPDHIANKIAAGEVVERPASVVKELVENSLDAGADEV
                     TITVRSGGKDLVQVIDNGKGMTQDDAVLCFERHATSKIRQWEDLLKVQSFGFRGEALA
                     SIASVSDIELKTRMPDQEVGTLVQLHAGKLNNVTQEASLPGTTITVKNLFYNVPARRN
                     FLKTNTTEFRHIAGVIKRFAIAYPAVEFTLIHDDEKIFQLRKTDLYGRLEKVIGREIM
                     SYTLPLMADQFGVKIHGYVSKPSFSYKTKGEQFLFVNGRPIVNRTANFSVASAYGHTI
                     PKGDKPFFVLFLEWNSEEFDVNVHPTKAEVKFKDEQLLYRVVFHAVRDTMNTDVMIPS
                     LTQSHPMHSSSNDIRTSDGTAMMLELNTQRQPLQWTTPAHSNTAPQPTSDEAVAQFFS
                     AEETAPSPKSYIQDQLVKRIPVNLNSMVWQLHNRYIVSQIKSGLAIIDQHVAHERILY
                     ERALDAFEHNPIFSQQLLFPQTIEFSPEDFSIIEKHIALMERLGFVVKVFGKRTLVVE
                     AVPADVRLGNEQKILTEIIREYKEHEHDAMDPRDNLAKSFACKSAIKSGDPLNVDEMN
                     GLIDQLFACKFPYVCPHGRPIIIQLTLDELDKRFMRT"
     gene            complement(10982..11908)
                     /locus_tag="PK937_06505"
     CDS             complement(10982..11908)
                     /locus_tag="PK937_06505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH32256.1"
                     /translation="MSGSLRFETVLCPVCSHDQYSPWMQFNDKYAEHTLVRCTQCRFV
                     YINPRPTEDTIGVYYSAEEYTPFVSSSDNRSLFAKVYHTVRRFSVRWKRKKVENKSNK
                     GLVIDLGCGTAEFLNEMKQNGWQVAGIEPSPEASEFARSNYDIPVLTGTINRELIGQT
                     PDNPDVITMWHVLEHVHRLDETLDLIYKKLKPGGTLVIAVPNINSLDALFYGRDWVAL
                     DVPRHLYDFVLADLRSLLNKHQFRVYRTHGMPIDAVFNVLMSEKNIIQRRGLWFLPLS
                     LVRIPLVVSITTMAGLIRGRASAIVVYAEKQG"
     gene            complement(11915..12283)
                     /locus_tag="PK937_06510"
     CDS             complement(11915..12283)
                     /locus_tag="PK937_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32257.1"
                     /translation="MQRIFGFLIFSFLMAGCGNTSRLIVYTDANYAMVEKGKNIRVKH
                     VDGKFVQFYKMIIDKTDDEYIYARCWKTKKSTPEDLRFKKSEVILESTEFSVSRTTEY
                     AMLIGVTILLIFLSDRILRG"
     gene            complement(12294..12566)
                     /locus_tag="PK937_06515"
     CDS             complement(12294..12566)
                     /locus_tag="PK937_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32258.1"
                     /translation="MQQSGLMLKIGGWFSAVLLSVGLLIRLSGNLALSHFVLISGIVV
                     MLISPLAGMLVMIVKFWKNGEIRFAYIGVLILFLLAGGTYILHILQ"
     gene            complement(12568..13401)
                     /locus_tag="PK937_06520"
     CDS             complement(12568..13401)
                     /locus_tag="PK937_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNH32259.1"
                     /translation="MEILAYLIIGFTAGAVGSMVGLGGAIIMTPALTILLNVPIKSAI
                     GASLIALIATSVMSTAVYSKKELIHYRYGMLLLILMVSGSFIGSAIGVMMDARLLSLF
                     FVALMIFAAAFLLYQVFQNENPDARVEWQNDEEPHRFPPNGVLLEAHSGIRKFYRVQH
                     GVVMMFISGIAGVISGMLGVGGGIIQVPFMHAINRMPIKAATATSGFMIGYTGMAGAL
                     VYVIFGQFELIMTSALVLGILTGSQAGARLAIRLHARWIIYLVIVVLTVAAIRLFMKV
                     F"
     gene            complement(13409..15157)
                     /locus_tag="PK937_06525"
     CDS             complement(13409..15157)
                     /locus_tag="PK937_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNH32260.1"
                     /translation="MKFTFNRVKTLIVVSALSVTFIGGWFAESLIGDDYYYEVKRSLR
                     LFGDVYKNVGEKYVDEISPKVIMRTGIDAMLETLDPYTMFYEGSESSDLDAFMKGKYG
                     GIGVTVGIRRNTIMVITVTEGSPAYKAGLKPGDRIVGVDGKTTRDIPIRDLSNSVKGE
                     PGTSLKLDVKREGQSSALQMTMIREMIAIENIPYYGVLAGDIGIIKLTKFSKTSTSDF
                     TRALKDLRSQGIKGLIIDLRGNPGGLLDAAIDITNQFVRKGELITYTKGRIQDANRSY
                     YANNDPLIPQLPVAVLVDGNSASASEIVSGAMQDLDRGILVGSTTFGKGLVQTVYPLQ
                     ERDAVLKITTAKYYTPSGRCIQREHYAHDRSNAMRLESEDEPNEFYYDVFGEEDQVRD
                     SLTNDSLPSKRQEFKTKTGRIVYAEGGITPDIIKDDYSFLGYTREIVRRGYVFDFATR
                     FSAQNTRIDTGFVPDEELLTEFYKFLKDHHFNYNSTADSQLTALRAYAENNGYSAQIQ
                     QSILQLQNKFDEEEVKEFEAAKTAIRNLLQQEIISRYYGNRRRFEITLKSDPVVESAR
                     SVLKDEVLYKKILKQK"
     gene            complement(15168..17801)
                     /locus_tag="PK937_06530"
     CDS             complement(15168..17801)
                     /locus_tag="PK937_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNH32261.1"
                     /translation="MQLLMKLVLSLFFLIYSVCINTLNAKVKPPKNASTHKVQFVFAD
                     SLTGWAIGSIKTGGALKDSLNPKALFITGILKTYDGGLNWELQTVLGVEENGWEVRAT
                     ASLNRTHVWAILSKPNSDQNVLLRTTDGRRWTMQSLKLADGDPVQICFSDPANGFMIL
                     REFMGSTILRTTDGGATWATFEPGLGSDYRGVAFDGTSWYVLAQSRTKPEVVTVLRSD
                     DNGNILIEAETFNPESGDHILEGFGIRSHKNKISIMLRDIDSKTRETSRWFWIQSGDR
                     FVSHTSQAFQYEKEFKSPSDIQNFSIDHSIYVLDRSEEYEPGLPDAKIRYSLIRSVDE
                     GKSWTSIPEFRFPVLALQAVGDDVLISANDGNLYKFNYITGIYQTASVDLKKAFLNPP
                     QVGTESVAENGENIFDLGHDEFEKESVYESKWESANDSAFAESFSSVRDANKYKPDAK
                     AEFSVGIDSITSIGVVLHRRTRMGSKHQAINLINEELQKGEIRLRKYKPITFKGTVRS
                     TGTRFPVKYSWSSSISGELSDQIEFTTSPRQLPPGVHYVFFKAQDNLGTWSNPVVVKV
                     IVDDFPKYKFPFHGNWVLGGGGSHYNIGHHIRTVPYGIDVNNIEQGYGGDADIGFPIR
                     ASTDGIVSLAGYIKGYGRTVKIDYMYGGKKYTTLVSHLSTISVEVGEYVKQGQEVGTV
                     GSTGRSSAPHIHWELRVDGTNLPPEPIFENDTTILQKLPNGLHVSSDNIYEPEHIVIV
                     PEPEIPGTQWDHRGYHHGYRYMWITRGKPTVEAVWKPKLSKSGSYKIQVHIPKKFADG
                     VAFYRVSTKNGIDTVKVNQSKYTDEWITLGVYELDKNDEISVMLNNSTPQGKGSVAFD
                     DVRFVGQWDTYSKKGFNKPIQ"
     gene            complement(17805..20000)
                     /locus_tag="PK937_06535"
     CDS             complement(17805..20000)
                     /locus_tag="PK937_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32262.1"
                     /translation="MFSFWLSNKLRGSMLILRIIAPAMMAFIPLTYDLDYEFAEALTI
                     INVIFFAVWMSTERAHSHINYRYGFLRTVNRLLLPIIFFDLVIISIAMIAGHHLHWYR
                     TFGLYFLLPFVGSWIGLTLGYVCRFFIQRRPWLWVATILLSTLILNLIWLLFTAPIFV
                     YSIWWGYFAGPIYDEWIPLTDTLLFHRLFSLGLAFAIFISGDLITRKLNNTITYKKFV
                     FWITTVWLIITGIFYWRDNWGWDTSYATLEQKLNVVYQDERLTIYSDTTSKFITRYVD
                     ARIKQVTDWLNINPAKVKIFIYPDAQTKKILMGAGGTNFAKVWRNEVHVNSDDAEFTI
                     KHEAVHVLASSFGHPLYSTTKPGMLEGLAVAAAWDESQLTPHEWSAALYKLNKLPDIT
                     ALMEPMGFWSSSSGLAYTVSGSFVRFLIDQYPIQNIHRVYASEDFKDVYGLPLDTLRE
                     RWKFFLNTIELDSLDLRIATTLLQPSLFRRRCPHYVADLQKEYYASIEMKEFDGALKL
                     TEQIISTGTSPERWVLSRARIHFKQKKYDAALSKVEEVLNRSTLSYTMRGWALLLKAD
                     IVFCQGQIQKAMDLTHEIMSLYDQTTSVYEAALWSDHLMRHPGNGTLYASWLDAVDQN
                     KVGEWYSVHYDKKDDVIFSYKLVSYLYQTSQYDSIFSYIKTHPMAITDSSVYLKYNQL
                     LGDAAQRTELWSEAEQYYRVGLQYAKRTTEKVQFDDRLELIHYLKNKIE"
     gene            complement(20011..20883)
                     /locus_tag="PK937_06540"
     CDS             complement(20011..20883)
                     /locus_tag="PK937_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32263.1"
                     /translation="MISNNRFVVLVCILWLISSLGCDKNIKIIRDGFRLEYAINNPAH
                     AFHVRLVFETNPRDSLIGLMYIARTAKESGILDYREGHTLRQATGDTLFMNPSQFSDL
                     LECVNDLHKMIKPSNVYGQSDGKIVNINLRHSGKSYDWRLFGKTDQSSVKKLTAYIET
                     MFEETYPGISLSAENSRIELSLCNHANSKNTETNFSIYSNNNIIRVHQDSLSAYTLDA
                     GKHLYLWQVLEANDVWRLDDNKTMQGKYPSFYELKIQMGKRTHRWSAYAPIYLDDRRY
                     LRIVQAIESTVLSR"
     gene            complement(20880..23021)
                     /locus_tag="PK937_06545"
     CDS             complement(20880..23021)
                     /locus_tag="PK937_06545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH32264.1"
                     /translation="MTNESYPEKIGRYQILSLLGEGAMAKVFKAVDTRIDRVVALKML
                     QFREGMTDEMIQQLKDRFVHEAKFAGKLTHPNIVTIYDAEEENGVSYMAMEYVDGRTL
                     ESVIENNETLTVEQILDILIQLAHGLDYAHKHDIIHRDIKPGNIILTSEDIPKIMDFG
                     IAKITTSSTTVIGTILGTPGYMSPEQITGKSVDHRTDIFALGAVFYELLTREKAFPGK
                     NLTEIMYRVVSENPMPPSMVNLNVPEIINPIVMRAIAKGPGERYQSGTEFAKALEVTK
                     KQLSSGGHRTTISKSVPPSADKKTEIVRFFENRYVGMVSFGWATLATILLLITWSGGF
                     EFSRMARSLSEKGPASLIMNLNVPDAEIMIDGDRYTPQSSLFRLDSVGVGERRITVKR
                     DHYESYETAVIFGKGEQKNIEAHLKPLPVEIPPGSDTSFVTIRSSPDMVKVETSYGKF
                     LGFTPIDSLIFPGGRYTLIFSREDFISRKRDIALGKKRLTYVEQSLEKQRGTVSLDQV
                     FPEQSYLMVNGRRIQKNYRNNSYVLPVGNQVLTIKADGYDDLVKSLRVEPDSVLVLTD
                     SLKAQWTSVRFESNPTGADVYLDGSEESVGKTPVIVNDLLANSHKLRAVLRNEKRLMN
                     FRAVKGDTATVRVVFANPNGYLNLVSDPPGAEIFLNTVRDDDYKTPVKRELKPGFYKV
                     RLTHPSFKKYHEITVRVRPDFTTELVHKFEP"
     gene            complement(23056..23730)
                     /gene="cmk"
                     /locus_tag="PK937_06550"
     CDS             complement(23056..23730)
                     /gene="cmk"
                     /locus_tag="PK937_06550"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNH32265.1"
                     /translation="MKKLLITLDGPAGSGKSTTAKLLAKRLGYCYLDTGAMYRAITLQ
                     AIHHGIFPTDPEKIIAMLPDITLHIDYKDGRQQTFLNGWDVSEGIRSPEVTRHVSAVS
                     AIAEVRTFLRQQQQHIGNQGGYVVDGRDIGTVVFPNADIKFYMTANVEERARRRLKEM
                     ADTDEWDLPKMIEDIQKRDHADMTRAEAPLRQPPDAIVIDNSLMTLDEQIQAIITHIE
                     NRFGNQ"
     gene            complement(23741..24451)
                     /locus_tag="PK937_06555"
     CDS             complement(23741..24451)
                     /locus_tag="PK937_06555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNH32266.1"
                     /translation="MRLNKFMATAGVASRRSCDEIIGEGRVTVNGQTVTDLSYQVDEA
                     LDRVEVDGQLLSIKEKDVYIILHKPLRYVTTVKDERGRDSVVDLVKITERVYPVGRLD
                     FDTTGLLLLTNDGELAFRLAHPKFGIEKTYIALLNKKIGESELQQLRRGVVLIDGKTA
                     PCKAEKLGKELVKITIHEGKNKQVKRMFRKLGYKVRELHRSQYGPIQLGKLKYGQWRN
                     LKPAEVLALKKATGLIKK"
     gene            complement(24458..25291)
                     /locus_tag="PK937_06560"
     CDS             complement(24458..25291)
                     /locus_tag="PK937_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32267.1"
                     /translation="MNFDYLNDQTWIVNQDIPWKHLSKDLSEVRIAIITTAGLYMWKT
                     QKPFSFEDEIPDVSFREIPNSVTQETVRVSHRYMDLFGSAGVDINSVFPIHRLQEMVS
                     EKRIGGLAETHYSVMGEVRDPRLFIEDCMPKILKLLQKQLVDVVLVTPGGPLGHQTAG
                     MIARFLEENDITTITIGAVKAIAQNVKSPRTLLVRYPFGMYFGAPFDTVNQKEIVNEC
                     LVHLKAIREPGEVSELSVRWQDTFKTALQIKPELPTLMDTGAIIAEPVIEKKKIAADE
                     F"
     gene            complement(25298..26101)
                     /gene="scpB"
                     /locus_tag="PK937_06565"
     CDS             complement(25298..26101)
                     /gene="scpB"
                     /locus_tag="PK937_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNH32268.1"
                     /translation="MEEQNQNESNLEPTEVQIDTETSEVPADPRIVDGLPLQNVVESI
                     IFASTEPLPMDRLLECIQKDYVQASLVRQVIDEINKHYQANNLPLRVIKIAGGYQYAT
                     HPQYDKWVSRLFKSKAEKKLSQSSLEVLAIVAYRQPISRAEIERIRGVNADWTLRSLM
                     EKNLITVVGREDAPGKPLLFGTTKPFLEHFGLDAINELPKLKEIEDIIKEDKEFAETL
                     EFDFADKKAAEAAAKPADETAKNEGLSQEFAEIDTILKENNFNPEDHLK"
     gene            complement(26120..28393)
                     /locus_tag="PK937_06570"
     CDS             complement(26120..28393)
                     /locus_tag="PK937_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNH32269.1"
                     /translation="MYRVKLEQFEGPLDLLLFFVRKDELNIYDIPIAYITKQYLEYLH
                     LMRTLNLDIAGEFILMAATLMRIKAKMMLPPDPTTEEEEELIDPRQELSRRLVEYRQF
                     KEASKSLAELDEYWRSVYRRSYFNFDLIPQQTEEAVGLKDISFFDLLTAYKNAMAKKP
                     AVIYHNVERLNVTVEEQQEYIMDFFRDRNMYTFLELVEDMSKIEVVVTFLALLDLVKK
                     GEIAARQTTLFDDIWIYKASAYTDEIPDELRAEESETEKTIPSNDEPVLTPSEAPVLN
                     EVSSNDTTFAINESGDTALDTSLIEVAASPDSELANDHIDMAQTDGVVQEVEQAAVEM
                     SIESVIEAAKTDAETVELNEEILNNEAFVSAPQTEEQPQTEYTDTSEAFADSENDEII
                     GATVEENLNSLSDENIYSVEEIRSNSSSDETISVSVNESTEVDFYTQVPSEVTSAEQN
                     QSDISDVKTENMGSHGEDFSHEETELLDRTIENVSDDGIATDLTASISDATVDGNVTD
                     DAVLLISTTEDQTIASPVPVELIRTVIAESQVNVNNSDLESADEITGNLALSDTEDKV
                     IVPTNEPIVGEAETNTTDIDQTPPQVSSNEIGDNINADQSASDTIISVEENEPALIRS
                     NDVSTPDIQAIHENELTSEDVVESITSEPPQNTDPVHIIEAIHNQNYETIQPDLVKPL
                     VSQDAPRTDGESVVSAKMEDDVSKPLVEQHETADAFLSRTTTNDEKPSLDETEKPSII
                     TVFVRKIVSFVKRFFGK"
     gene            complement(28403..29842)
                     /locus_tag="PK937_06575"
     CDS             complement(28403..29842)
                     /locus_tag="PK937_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNH32270.1"
                     /translation="MRYVLFWILSISTLCAQDQIQPYKDYDNDWLSPQFHKGRRDALA
                     QKMKPHSIAVLFSSEERLRSNDTYYQYKPESNFYYLTGFREPNAALIYLPEGWVVGGK
                     TYSTVMFVPKRDYRYEVYNGRRMGTDGTQKILGIDTAFTNDEFSTQLNKILLQQLAAS
                     KLENVYCFNLREQYDTKTLRNMTTAFTDFRNNPRSLLGYMNSVDFINVEPLIGELRAV
                     KTDEEIVLLKKSIEITNDAHLQMMKSCEPGQKEFEIKAVGEYVFTRLGAEYEGYGSIC
                     GSAENSIILHYTTDRRQMKDGDVLLVDMASEYHGYSADVTRSYPVNGKFTKEQTIIYN
                     IVLKAQQAGIDRIKTNVPYREVADAITQTLEDGLLEIGLIQDRKEAKKYTVHGYMHSI
                     GLDVHDPQSYDKPFVPGFFTTVEPGIYIPENAPCDKKWWNIGIRIEDDILVTESGNIN
                     LSERVPRKIEDIEKLMKKKGVGNVPLDNN"
     gene            complement(29862..>30298)
                     /locus_tag="PK937_06580"
     CDS             complement(29862..>30298)
                     /locus_tag="PK937_06580"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNH32271.1"
                     /translation="TPRLPGVDGNAKMSKSLGNCIYLSDDDATIESQVRKMVTDPQKL
                     RKNDPGRPEVCSVYSYHKVFNTDETQGIAAGCTTGELGCVACKKNLGQKLSQRLEPMR
                     QKRAELVSDLHAVEKIISEGSDKARAIASDTMVSVRRAMKLD"
BASE COUNT         8244 a         7058 c         6284 g         8712 t
ORIGIN      
        1 cgcagttttt ttgcgcaggc ctcggtaagc ggtaaagtta tactcggttc gaccaaacaa
       61 cctgtaaaag cagattgggt aacactcatc aaatccggcc aaggattaca gacattgcag
      121 caattagaca atgtatcgga atacgctttc aaagatattc cgctcaatcc tatggctccg
      181 tatcttattc gggcttcata caaaggcatc ctatacacac aaaatgtgat gatcaatgaa
      241 aacaaaccgt atatcgccga tgtatccgta gccggtgtca gcgatcaatg gcgcgatata
      301 gatgttcgcg ttccgcatat gatcgtcgct cgtaccgatg ataaactgga aatcgaacaa
      361 acttttgaaa taactaataa cggcgccaat acttttaatc ccaaagatac caatgtccct
      421 tcatttttgt ttaagcttcc ggacggggta aaattggaag ccgttaccac ctcttttgac
      481 aaaagcatgc caatacctgc ttcggtggta aactataaag acaagcaagg tatcattttt
      541 ccattacgcc ccggcactac acagatacaa atcacggcat cggcgccgta tgcttcgccg
      601 tctttaacat gggcgcaacc tatgtattac gatgtatcgg aatttcgtat cttcgtcacg
      661 cccaaagaca tcgaagtcag cggtgagaat ttccaacata ttcatgatga acaattggaa
      721 gcaaataact tttcgattac ggctgctact aacctgaaat ccggaagtac gataggtatt
      781 agcttcaaag gcggcagtgc gcgcggcgga gcgccacaag aagatgatca cccgatagat
      841 gccgctgcca acgaaattca gcgcaatgtg ttcttccttg ctcccctttt gatgtcattt
      901 cttctttttg ctttgtatat cgcactcaat cgaaaacatc aatccgatgg cggcggtgcg
      961 caacaaaaag cacactggat ggccgagcaa gaaaaactga tcgcatccat cgctcgatta
     1021 gatgatgaat atgctgagaa ttcggacaac agtgagtatg tagatcggcg acgcgatctc
     1081 aaacatcagt tgcgcgatgt ttcaaagcgc atccaacgtg ccggcgcatg atccgattac
     1141 agcacatttc gaaacaattt ggccgtttta aagcgctgga tgacctttcg atagaaatcc
     1201 cttcgggtga tttcatggcg ttgctaggac ctaacggtgc aggcaaaacg accacgctca
     1261 aactacttgc aggattgatc cgaccttctt ccggcgccat ccaggtggac ggtatggaaa
     1321 aaaacagtcg ctccgttttt ttacaatcca tcggcatggt atcccaccaa tcttttttgt
     1381 acaatgatct gacggctatt gaaaatttgc gtttttacgc taatatgtat actgtttcca
     1441 atgcctctgc acgtatcgcc tcacttttaa aacgtgtcgg attgggtgcg cggggtgatg
     1501 atatagttcg caccttctca cgcggcatgc tgcagcgcct gtctattgca cgggcacttt
     1561 tgcatgaacc gcaaatgttg cttttggatg aaccctatac cggcctggac gtgcatggtt
     1621 gccgcttttt agatgaagtg cttaaagaat accatgccgg cggccatagc atcgtaatga
     1681 taacgcacga tctggaccga atcctaggat tgagcaaccg tatcgtcgtt cttgacaatg
     1741 gtcgtgctgt atttgaccgg cgatcggtgg aaccggaatt ttctactttt aaaaattata
     1801 taaggaatta aagctttgaa tcaggaatcc ctgcgcgtaa ttgataagtc taattattat
     1861 gatgtcctct gccattttga tcagcacatt acggatgcac tagataggtt tgacggtcaa
     1921 aaaagcaacc tttctggttt atcaaagagc cgtgctggtt ttcgtaatat cttaatttgc
     1981 ggtatgggcg gttcggccat agccggtgat tttattaaaa acgtttggac agaagtttta
     2041 ggtttgccga ttcaagtttg ccgtgattat catattccgc gttgggtcaa taaagaaagt
     2101 ttagttattg cgtgcagtta ttcaggcaat acggaagaaa ccattgcggc cttacacgaa
     2161 gccatggaag cgcaagcgca gattcttacc attgcaaccg gtggtacggt tcagtccatc
     2221 gcaaatcaac atggattatc gcatatcact ataccgggtg gtttgcagcc acgccaagcc
     2281 atcggttatg cacttacgac actccatgcg gttttcagcg aaatattcaa atccgccccg
     2341 gttcgtgaag agatgtctca catcgttttt caaatgaagc acctcgcaac catcctcaaa
     2401 accgtccacg ataataatcc ctatattcac gacagcaaaa tactgcatga caagccggta
     2461 atcatctatg cgtcggaacg tatgtttcct gcggcattgc gactcaaagg gcaaatcacg
     2521 gaaaatgcta aactattagc ctttgcttat gccgttcccg aaatgaatca caatgaaatc
     2581 gtcggttggg aaagcgtaca cgaagcccat catgctccct gtcatgtgat cttattaaga
     2641 gatcgcgaag atcatccgca ggtgcaaaaa cgctttgaaa tattgatgcg attgctcaaa
     2701 gggaaagccc attggacgga atacacgtcc gacggcggga ccttgatgga gcgctattat
     2761 aatctcatat atcgtggcga ttggctcagc tactatatgg cgattgcccg aaatacggac
     2821 cccacaccgg tagatatcat cacgcggttg aaaaacgaac tcgcgtctta gattagcatt
     2881 tattaaaaaa acgcctgcac ttcggccgtt ccgagatgaa tgatcttttg ccccgggtat
     2941 tttcgcccgg tataatttgc ccgcatgatg acgtgttcgc cggccctata atccacggcg
     3001 gaaatccata accaattcca tcctcgtaca cggccatcgg tgacttcaaa cggagtatta
     3061 cctaatccgt ctttcaacca aaggcgtgtc agttcgaggg tattagtcca tcgcccgcgg
     3121 ttgagccatg aaaaaaccat cgtcggctgc caattcatga ttttagcgcg acttccggtt
     3181 tcatagtcct tcgccaacag ccaatcggtt cgcaaatgat actccatcgt cggttttggt
     3241 ttgaatacga tacttggcga gtatctgttt ttatgaatgt cataccggat attgaggctg
     3301 cttacattga tcaaagattg tctttgggcg atcgtgactt ctccttcttg ctcccatgac
     3361 caaaacgaat tcacttgttt ttgaatgcgt aaataataga tatcgcgttg attcttatcc
     3421 tgtccgtcat tcaaaagacg attacgtgta cgattggttt cataacgcaa tcgccagagc
     3481 atatcgtaga tccgatcgta gccgctcaac tcttgccgca aaaggagatt cccccgttgt
     3541 gtatttttgt tttgaaccgc ttggagccaa taaaagcgac gatcgggatc tgtttgattt
     3601 tcttcgatgc gaaaaagacc ttcataaatc aaacgttcta taaatcggga ttgccatcta
     3661 tcacttatag agtcttttga agaacgactc cgcaagcgac caatttccaa acgtagccga
     3721 attccagatt ttagttccac cacaggagta aatccttgac cgcgaacggc gctttggatc
     3781 caatcgcctt gcccttgcgg aacctgcctg aaactatctt gacgcacttg aacaaaattg
     3841 cctgtattgg gcggcaccgg aataaaaacg atttttcggt tctgcaattg ctcctgagat
     3901 atctgataat caacggtaac ttgcacggct ctggaaaaag gatgataatc ggcgcttgcg
     3961 tttaccagat tactgatgcg atccggattg gctatcgttt tcttacttcc aaaatattct
     4021 ctttttcttc gttgccattc gaacgtgtga ttgagattat gccattccgt ccattgccaa
     4081 taataacgtt ggtttataga ttttgatacc cttccatcga gggaatccac ggcatcgttt
     4141 cgaatgcggt ccgatcgcat ttcataactt gcgccaaggg taagatattg accggtaatg
     4201 atgtttaaat tgggacgaat cacctgatac gaagcgccgt aaaccgaatc cgattgtctg
     4261 tttttttgcg attcgtcttc gtaatacaga cccggccgcc atatccgcca cgcgccattg
     4321 aaatcaaaga attttctttg tgtatggtta ttcactatga cggactgtgt ccgatccgta
     4381 atctgtatat catgaatgct gcccgatacg aaaatatttt tccgatctat ttttaagccc
     4441 gacccggatt tagtggatac aaattgatga tcgccgcgcg ttaattcacc cttaaaaaaa
     4501 tcagtttgta cgcctttcca cggcttatat gccaatctga aatcattcac ggtttcttgt
     4561 aatccgggcg cggttaccga atatacggac gtcgctggat tgaagatatt ccattgacga
     4621 ccgtattccg catcttgtgt gcgatcgggc gcagtaaaat tgctatcctg attacggcgt
     4681 ataatttgta aatcaaattt tccaagtttc gttttaaata aacgaatgtc ctgatctgca
     4741 acgaaagctt ccaattggta ggcataacct tgcgattggc gtggggaata aagattttta
     4801 tcaaattgac tcaatgcgaa ttctgttcgc aaccgcagat tctgaaccgg gttccatgcc
     4861 gcataccata ttcccgttct cagccgtgta ggcagatcca tcggtacgag cggtaaataa
     4921 taacccaaac ccgatccgac atagataaat tcacccaaaa tattaccgcg gatgtaatca
     4981 ccgcgtccag acccgacatc ggtaaaacgc acattgtacg agccggagct gtccgatccg
     5041 gaataaacgt aaatgctatc gttcgaatta gcatcataga ctttgtaata agcgccgcgc
     5101 ccttccccga cccatcgtcc tccatctacc agtatcgtat tcccattgcc ttgtagcccc
     5161 cgatcatcca acgatgcgag cgaatcgcgt aactgttggt caatagacag attaagcgga
     5221 ttattcttat catcggactc tgatagaaat gcgcctccta cagttaaacg tcggtcaaaa
     5281 aattgtgaac gtaccgatgc tgaagctgta ttacgtctaa acacttcatt ggaatattca
     5341 aaatcgacga tgattcttga ttctggtgtg accaaacgtt ttcgtgtaaa cgtgatctga
     5401 cctgcattat aatctatcac ataatcatta ttttcccctc gagtcaaggc tacaccatta
     5461 agccacaccc tttcgctacc ggcaacgata atcgggtttc gctcaccgtt ttttccggtt
     5521 aattcgtaag gcccttgaac gccttcggtt atggttagcg tattactgtg gtatttgcct
     5581 tttgccgttc cgtaagctgc agccgcgtcg gtacgtccat cgttgtacaa actacgaacg
     5641 ccttgaagcg aacggtgaaa attgccaaat tgggtgcctg ttgtttgtat ttcaaagtca
     5701 ccgagggttg cggtaaattt atccgcttta cgtacttcga tatagatttt atcgatttcc
     5761 tgaaggcgtt cggtatttcc ctcgggctgg attggcgttt gttcgtctgt tattaccgct
     5821 tccagcgtaa catcttcccc gatttggcct gtaacctgca cattcaatcc ggaatttaga
     5881 gtaaaatcct gattgttgcc gatcgtaaat ccgcgcgtaa ttgtaccacg gccattcaaa
     5941 tcgttggtaa actccgatga cggattcggc ggtgtccatc gcggtatggt atccgttttg
     6001 gatgaaatga tatttgttcg aataatcggc agatcacggc gggcatacga atcatcaatg
     6061 cctaatcggg aaactaaaaa acggcactca accgtaatcg aagaatctac gacaaatgaa
     6121 aaacgaacga caccatcgga ataacgtatc gtataatctt tttcgcgggt taatttctga
     6181 tatccgatag taaaaatttc actttcgggt atgatgcggc gaaacggaag atagtacaat
     6241 gtatcggaag aatatccgcg aatcgtgtgt gcgccttgaa aatagttaat atttttggat
     6301 agcaaggtgt gctgtattga ggagtctgcc gaaatgtaag gaggcgtctc atcctgtgca
     6361 ctcgccacgg ataaatcgat aacaatggcc gatagaaata taacggcaaa acgataaagc
     6421 atctatcgcg attcctcggt aatttcgatt tgatagagct ttgattcccc gacagtcgat
     6481 gactggtcta ctatccaaag cgagtgaccg aaacaagtca ttgcggtagg attttcaaat
     6541 gcaaaagcta ttgtcaaagt cactctctgc ccttcggcat tccatatcgt taacgatttt
     6601 ttgtcgtcat cccatatcaa cacacgatcc tgttcatcag ccgatagaag taagtttgcc
     6661 gaaaaaaatg tatcgtttat ggttcggata taatttccaa aaatatcgaa aattttgaca
     6721 ccggttcgat ctttgataaa tagatttttt ttaccgataa cgatctgttt tggatcgtca
     6781 agataaccgc cgcccgcagt aaaactattc cattccgtta tagtgcccgt gaccgaattg
     6841 actttgtaca cacgatttgt cgcctgcgat atataaaaaa gatcgccctg tttgctttgg
     6901 gttagtgaca aaggataagc cacgctatga acggattctt ggcctgagta atcgagatta
     6961 tgcccgccga tggaagccac ccattccaat cgcctattta gccggacaat ccgctgattg
     7021 ttcatatcag ccacataaag attgagtcca tcatgagagg taatccctgt cgggtaatcc
     7081 agcatttcac cactccagcc taacccacca aacaaaccaa gcatttgacc ttcagtattc
     7141 attcgaagga cacgatgccc ggacgattcc gtcatataca cttcgcgcaa aggagtaaca
     7201 catacggaat tgatctgtgt aacattgtta ccgtccggtg ttttgatggg aattacctgt
     7261 ttaatctgaa aacgtatttc ctcttgccca agaagaggaa cgcagcagat cattaagaag
     7321 atacaaatcg ttttgaattt cacgtttttt tccgatagat tggggtcgct taaataatta
     7381 ataaaggagg ttcccttgct gttccgtaat ttcatatcgt tgagtaaaaa catatccatg
     7441 ctgttattag gccttacatt agctaatacg gcttgcacgc aatcggccaa aaccactgag
     7501 aagaagaaag taaatgcgga agtcgccgtg atcgaaactg attttggcaa tattataatc
     7561 aaattttacg ataaagaagc gccgaaacac acagaaaatt ttaaaaaatt ggcccgcgcc
     7621 ggtttttatg atacgctgac atttcaccgt gtcatacccg gatttatgat tcaggccggt
     7681 gatcccaatt ccaaagataa tgacccttat aatgacggtc gcggcggtcc gggttatacg
     7741 gtgccggcag agtttaataa aaaacgtttt cacaaacgtg gcgcattagc cgctgcgcgt
     7801 caagccgatc aggtcaatcc gcgaaaagaa tccagcggtt cacaatttta tatcgtacaa
     7861 aacggccctg tatctttaga tgtactcaaa cagatggaag cgcagtttaa agcggccaaa
     7921 ccgggtttct atttcaccaa tgaacaaatt gacacttaca ccaccttggg cggtacgcct
     7981 ttcttagacg gcgaatatac cgtgtatggt gaagttgtca gcggtcttga tgtcatagat
     8041 aaaattgcta tggtacaaac cgacccacgc aataatcgcc ctgttcagaa tgtaattatg
     8101 aaaaaggttt ccatacaaac gatggagatc gaagagtaaa aattaaaacg gccactccgg
     8161 tatatgttat tgacatgacc cgggtggccg ttacttattt aatataaatt ttattcttat
     8221 ttgttcaggt tgaaatgctt taatgtattg tccaatattt tctccgggct atttttatcc
     8281 gtcataatcc atcgaatgcg ttcgttcttc ctaaaccatg tcaattgacg tttagcgtaa
     8341 ttacgcgatc cttgtttaaa taaacgcacc attgtatcgc aatccattgt ccctttcaga
     8401 taagcgataa tctctctata tcccaaactt tgcatcgaga tcaaattttc cgaatatccc
     8461 cgttcaagca gcatctcggt ttcaccgatc accccttgtt cgatcatttg atccacacgc
     8521 atttcaatac gttgatacag ttttgttcgg tcgcaatcca atccgaccca cagtgaattc
     8581 caaggaaact tggattgggt ttccttgtgc cattgggaca gcggtttacc ggttacacga
     8641 aaaacctcca gtgcgcgctc aattctttgt acatcgctga tatgcaaccg gttcgccgtc
     8701 tcggggtcat tttgccgtaa ctcttcaaaa actgatacaa taccttcaga atctatttgc
     8761 tttcgtatct cagcacgcac gatctcgtta ttatctacgc cttcaaaaaa tccatcaatc
     8821 aaagcacgaa tatacaatcc cgatcctccc acaatcaccg gttgcttacc gcgatcaaag
     8881 atggattgaa taacgggaag tgcttcacgc gcatattgcg cagcattata ggtttcgtcc
     8941 gggttgatat gatcaataaa atggtgcggg acacgaaggc gttgtgtgtt atccggtttg
     9001 gccgtacaaa tatccatata gcggtaaagc tggcgggaat cggcggaaac gatttcaata
     9061 tcaaaatatt cagaaaggtc tacagccaaa gccgttttac cgatcgccgt cggtccgaca
     9121 atcactaata cattgggatt caaatcgttc ataaaagatg cgccttgctc aggtgcgcat
     9181 aaaacgtttg tccaattcat ccaatgtcaa ttggatgata ataggtcgcc cgtgcggaca
     9241 tacgtaaggg aatttgcagg caaataattg gtctataagt ccgttcattt catccacgtt
     9301 caaaggatcg ccagacttga tagccgattt gcaggcaaat gatttggcca gattatcacg
     9361 gggatccatc gcgtcatgtt cgtgctcttt gtattcccgt ataatctccg taaggatttt
     9421 ttgttcatta ccaagacgaa catcggcagg aacggcttcg acgaccagcg tccgtttacc
     9481 gaatactttc acgacaaagc cgagccgctc catcaaagca atatgttttt caataatgga
     9541 aaaatcttca ggagaaaact caatcgtctg aggaaaaagt aattgttgtg aaaaaatcgg
     9601 attatgctca aacgcatcca gcgctctttc atagaggatt cgctcatgcg ccacatgctg
     9661 atcgatgata gccaaaccgc ttttgatttg cgaaacgata taccgattat gcagctgcca
     9721 aaccatactg ttgaggttga ccgggatacg tttgaccaat tgatcttgta tataactttt
     9781 ggggctcgga gccgtttctt ccgctgagaa aaattgcgct acggcttcgt cggaggtcgg
     9841 ttgcggtgcg gtgttggaat gcgccggtgt cgtccactgc aaaggttgac gttgtgtatt
     9901 tagttccagc atcatagccg tgccatcgga ggtacgaata tcattggaag aagaatgcat
     9961 aggatgagat tgggtcaggc tgggaatcat cacatccgta ttcatagtgt cccttacggc
    10021 atgaaataca acacggtaca aaagctgttc atctttaaat ttgacctcgg cttttgtcgg
    10081 atgcacgttg acatcaaact cttctgaatt ccactccaga aataaaacga aaaacggttt
    10141 atcacctttg ggaatggtat ggccatacgc cgacgctaca gaaaaattcg ccgtgcggtt
    10201 cacgataggc cttccattga caaaaagaaa ttgttcacct ttggttttat atgagaacga
    10261 gggtttactt acatatccat gaatttttac gccaaactga tccgccatta gcggcaatgt
    10321 gtatgacatg atttcccgtc cgatgacttt ttccaggcga ccgtaaagat ccgttttccg
    10381 caattggaat attttttcat catcatggat caaggtaaat tcaacggcgg gatatgcgat
    10441 tgcaaatcgt ttgatgacac ccgcaatatg ccgaaactca gtcgtattgg tctttaaaaa
    10501 attacggcgc gccggtacat tgtagaacaa atttttgacg gttatcgtag tacccggcaa
    10561 cgatgcttct tgcgttacat tgttcaactt tccggcatgc aattggacta gggttccgac
    10621 ctcctgatcg ggcatacgtg tttttagttc gatgtcggag acggatgcaa tactggccaa
    10681 ggcttcccct cgaaaaccga aactctgtac tttgagcaaa tcttcccatt ggcgtatttt
    10741 gctggttgcg tgcctttcaa aacaaagcac agcatcgtct tgcgtcatac ctttaccgtt
    10801 atcaatcacc tgcacaagat cttttcctcc ggatcggacc gtgatcgtaa cctcatccgc
    10861 accggcatcc aaactatttt caaccaattc cttgactacc gaagccgggc gttcaacgac
    10921 ctcaccggcc gcaattttat tggcaatatg atccggtagt atttgtattc gattttccat
    10981 gttatccttg tttttcagca taaactacga tagccgatgc tctcccgcgt atcaacccgg
    11041 ccatcgtagt gatagacact accaatggaa tgcgtaccaa tgacagaggg agaaaccaca
    11101 aaccgcgtcg ttgaataata tttttttcac tcatcaatac gttaaatacc gcatcaatag
    11161 gcatgccatg cgtgcgatat acccgaaact gatgtttatt taacaacgat cgaagatcag
    11221 ctaaaacaaa atcgtacaaa tgacgcggta catccaatgc cacccaatcg cgtccgtaaa
    11281 aaagagcatc taatgaatta atattaggta cagcgataac caaagtgccg cccggtttaa
    11341 gctttttata aatcaaatcg agtgtttcat ccaaacgatg cacgtgctcc aatacatgcc
    11401 acatggtaat aacatcaggg ttgtccggtg tttgccctat gagctcccga ttaatagtac
    11461 ctgtgagaac aggaatatca tagtttgagc gtgcaaattc tgatgcttcc ggcgaaggtt
    11521 cgataccggc aacctgccag ccgttttgtt tcatttcatt gagaaactcg gccgtaccac
    11581 atcctaaatc aatgactaaa cctttgttcg atttgttttc tactttttta cgcttccatc
    11641 gtacggaaaa acggcggacc gtatgataaa ctttggcaaa taaactgcgg ttatctgacg
    11701 aacttacaaa cggcgtgtat tcttcggcgg aataatagac cccgatggta tcttctgtcg
    11761 gtcttgggtt gatgtataca aaccggcact gcgtgcaccg aaccagcgta tgttcggcgt
    11821 atttatcatt aaattgcatc catggtgaat attgatcatg cgaacatacc ggacacagca
    11881 cggtttcaaa acgtaaggaa cccgacatta tgcattaccc tctcaaaata cgatctgaaa
    11941 gaaatattaa taaaattgtt acaccgatga gcatggcgta ttccgttgtg cgggacacac
    12001 taaactctgt actttccaat atgacttccg atttcttaaa acgtaaatcc tccggcgtac
    12061 tttttttagt tttccagcaa cgggcgtaaa tatattcatc atctgtttta tcaattatca
    12121 tcttgtaaaa ctgaacaaat ttaccgtcca cgtgtttgac acgtatgttt tttccttttt
    12181 cgaccatagc gtaattagca tcggtataaa cgatcagtcg gctggtattg ccgcatccgg
    12241 ccattaaaaa actaaaaatt aagaatccaa atatacgctg catagatacc cctttactgt
    12301 agaatatgaa ggatataagt tccaccggct aataagaata atataagtac cccgatatat
    12361 gcaaatcgga tttcaccgtt tttccaaaat ttgacaatca ttaccagcat accggctaac
    12421 ggactgatca acataacgac aatgccgctg atcaaaacaa aatgagataa tgctaaatta
    12481 ccactcaatc gtatcagtaa accgacggat aaaagaaccg cgctgaacca accgccgatt
    12541 tttaacatca aacctgattg ctgcatttca aaatactttc atgaaaagtc gaattgccgc
    12601 aacggtcaag acaacaataa ctaaatatat gatccatcgt gcatgcagac gaatggctaa
    12661 acgtgccccg gcttgagaac cggtgagaat acccagaaca agtgccgaag tcataatcaa
    12721 ttcaaattgc ccaaaaataa catagaccag agccccggcc atacccgtat aaccaatcat
    12781 aaatcctgat gttgctgttg ccgccttgat gggcatacga ttgatcgcat gcataaaagg
    12841 tacctgaata atacctccac cgactcctaa catacccgaa atcacccctg caatacccga
    12901 aataaacatc ataacgacgc catgctgcac ccggtagaat ttgcgaatac cgctatgagc
    12961 ttccaataat acgccgttgg gaggaaaacg atgcggttct tcatcatttt gccattcaac
    13021 ccgggcatcc gggttttcat tttgaaaaac ctgatacagc aaaaaagctg cagcaaaaat
    13081 catcaacgct acaaaaaaaa gagaaagcaa acgggcatcc atcattacgc cgattgcgct
    13141 tcctataaaa gatccggaca ccataagtat caaaagaagc ataccgtaac gataatgaat
    13201 caattctttt ttagaataaa ccgccgttga catgaccgaa gtagcaatca gcgcaataag
    13261 gctggcgccg atcgcggatt tgatcggtac atttagcagg atcgttaagg ccggtgtcat
    13321 gatgatcgct ccgcctaaac ctaccatcga accgacggct ccggctgtaa agccaatgat
    13381 taaatatgca agaatttcca atggatagtt atttttgttt taaaatcttt ttatacaaca
    13441 cttcatcttt gagcacgctg cgagcggatt cgactaccgg atctgatttg agcgttattt
    13501 cgaaacgacg tcggtttcca taatatctgg aaatgatttc ttgctgcaaa agatttcgta
    13561 tcgccgtttt agcggcctcg aactctttca cttcttcttc atcaaatttg ttctgaagtt
    13621 gtagaatgga ttgctgtatc tgtgccgaat aaccattgtt ttcggcgtaa gcgcgtaatg
    13681 cagtcaactg cgaatcggcg gttgaattat aattgaaatg atgatctttg agaaatttat
    13741 aaaattcggt taacagttct tcatccggta caaatcccgt atcaattcgg gtattttgag
    13801 ccgaaaaacg agttgcaaaa tcgaatacgt acccgcgacg tactatttct cgtgtatatc
    13861 ccaaaaaact ataatcatct ttgatgatat ccggtgtgat gccgccttcg gcatatacga
    13921 tacgtcctgt tttagttttg aattcctggc gtttcgacgg caacgaatca ttggtcaggc
    13981 tatcccgtac ttgatcctct tccccaaaca catcgtaata aaattcattc ggttcatctt
    14041 ccgattccaa acgcattgcg tttgagcgat catgggcata gtgttcgcgc tggatacaac
    14101 gtccggacgg cgtatagtat ttagccgtag taattttgag aaccgcatca cgctcttgca
    14161 atggatatac ggtttgcaca aggcctttac cgaaagttgt gctacctact aaaataccgc
    14221 gatccaaatc ctgcattgcc ccgctcacga tttccgacgc actggcgcta ttaccgtcaa
    14281 ctaagacggc taccggcaat tgtggaatca aagggtcatt attggcatag tatgaacgat
    14341 tggcatcttg gattcgacct tttgtgtatg tgatcaattc gcctttacga acaaattgat
    14401 tggtaatatc aatggccgca tcaagaagtc ctcccggatt cccacgcaga tctatgatta
    14461 accccttgat gccttgtgat cttaaatctt ttaaagcgcg cgtgaaatcg gatgtagatg
    14521 ttttagagaa cttagtaagc ttgataatcc cgatatctcc ggccaatacg ccgtaatagg
    14581 gtatattttc tatggcgatc atctcacgaa tcatagtcat ttgcaaggcg ctgctttgac
    14641 cttcgcgttt aacatcaagt ttcaatgatg ttccgggttc gccttttacg gaattagaaa
    14701 gatcacgtat cggtatatca cgcgtcgttt taccatccac gccgacgata cgatctcccg
    14761 gttttaaacc ggctttgtat gcgggtgatc cttctgtcac ggtgattacc atgatcgtat
    14821 tgcggcgaat gccgacggtc acgccgatgc cgccgtattt gcccttcata aaagcatcca
    14881 aatcactgct ctcagaacct tcataaaaca tggtataggg atcgagcgtt tcaagcatgg
    14941 cgtcaatacc cgtacgcatg attactttgg gcgatatttc atctacatat ttttcaccga
    15001 catttttgta cacatcgcca aacagcctga gcgaacgttt gacttcgtaa taataatcat
    15061 caccgataag cgactcggca aaccagccgc cgatgaatgt aacactcagt gcgcttacta
    15121 cgatcagagt tttcacccga ttaaatgtaa acttcacaaa aggcctttca ttgaattggt
    15181 ttattaaaac cttttttact gtaggtgtcc cattgaccta caaatcgaac atcgtcgaat
    15241 gcaacggaac cttttccctg aggtgttgaa ttattaagca taaccgatat ttcatcattt
    15301 ttatcaagtt catacacgcc caaagttatc cattcgtcag tatatttgct ttgatttact
    15361 ttgaccgtat caataccgtt tttagtgctt acacgataga aggccacgcc atcggcaaat
    15421 tttttgggga tgtgtacttg gattttatag gaacccgact tagaaagttt cggtttccat
    15481 acggcttcca ccgtcggttt acctctcgta atccacatat accggtatcc gtgatgatag
    15541 ccgcgatgat cccattgtgt accgggtatc tccggttcag gaacgatcac gatgtgctca
    15601 ggttcatata tattatcgga tgaaacatga aggccgttgg gtaatttctg caaaatggtc
    15661 gtatcgtttt cgaatatcgg ttccggtggc agattggtgc catccaccct caactcccaa
    15721 tgaatatgcg gcgcactgga tcggccggtg cttcctaccg ttccgacttc ttgtccttgt
    15781 ttaacgtatt caccgacttc cacgctgatg gtcgatagat gtgaaactaa agtcgtatat
    15841 tttttcccgc cgtacatata atctattttt acggttcggc cataaccttt gatgtagccg
    15901 gccaaagaaa caataccgtc cgttgatgcg cgaataggaa aaccgatgtc ggcgtccccg
    15961 ccgtaaccct gttcgatatt atttacgtca ataccatacg gaaccgtacg aatatgatga
    16021 ccgatattat aatgactgcc gccgccaccg agcacccaat ttccgtggaa aggaaatttg
    16081 tatttaggaa aatcatctac gataactttt accactaccg gattggacca tgtgcctaaa
    16141 ttatcctggg ccttaaaaaa tacataatga acaccgggcg gcaattgacg cggactggta
    16201 gtaaactcga tctgatcgct gagttcgccg ctaatactgg aactccaact gtatttgaca
    16261 ggaaaacggg tacccgtgct gcgaaccgtc cctttgaatg tgataggttt atatttacga
    16321 aggcgtattt cacctttttg aagttcttcg ttgatcaggt tgatcgcctg atgtttagat
    16381 cccatacgtg tgcgccgatg taggaccacg ccgatagatg ttatcgaatc aataccgaca
    16441 gaaaactcgg ctttagcatc cggtttgtat ttgttagcat cccgtacgct actaaaactt
    16501 tcagcgaatg cactgtcatt tgcgctttcc catttgcttt cataaaccga ttctttttca
    16561 aactcatcat ggccgagatc aaatatattt tctccattct cggcgacact ttctgtacca
    16621 acctgaggcg gattcaaaaa agcttttttc agatcaacgg atgccgtctg ataaatacct
    16681 gtaatgtaat taaacttata cagattgccg tcattggcgc taataagaac atcgtcgccg
    16741 acagcctgga gtgctagtac cggaaaacga aactcaggaa tggacgtcca actttttcct
    16801 tcatcaaccg aacggataag actatatctt attttagcgt ccggcaatcc cggttcatac
    16861 tcttccgagc gatcaaggac ataaatcgag tgatctattg agaagttttg aatatctgaa
    16921 ggcgatttga attccttttc atattgaaac gcttgtgagg tatgggatac aaaacgatct
    16981 ccggactgta tccaaaacca ccgggaagtc tcacgcgttt tggaatcaat atcgcgcagc
    17041 attatggaga ttttattctt gtgtgagcga atgccgaatc cttctaatat gtgatcgccc
    17101 gattcaggat taaaagtttc ggcttcgatc aaaatattac cattatcatc actgcgtaaa
    17161 acagtaacca cttcaggttt agtacgagat tgggctaata cataccatga tgtaccgtcg
    17221 aacgccacgc cgcgataatc ggatccaagc cccggttcaa atgtcgccca agtagcgccg
    17281 ccatcggtcg tgcgcaatat ggtgctgccc ataaattcgc gtagaatcat aaacccgtta
    17341 gccgggtcgg aaaagcaaat ttgaaccgga tcgccatcgg caagcttcaa tgattgcatg
    17401 gtccagcggc gaccgtcggt cgtgcgtaat aatacatttt gatctgaatt gggtttactt
    17461 aaaatcgccc aaacatgcgt tctattgagg ctggccgttg cacgcacttc ccacccgttt
    17521 tcttcaacgc cgaggacggt ttgcaattcc caattcaatc ccccgtcata tgtttttaaa
    17581 atacctgtta tgaaaagcgc cttaggattt aagctgtctt ttaatgcacc accggtttta
    17641 atggatccga tcgcccaacc cgtaaggcta tccgcaaaaa caaattgtac tttatgcgta
    17701 gaagcgtttt ttggaggctt tactttcgca ttaagagtgt ttatgcaaac actgtaaatc
    17761 aaaaaaaaca gggataaaac taatttcatc aaaagttgca tgcgttattc aattttattt
    17821 ttcaagtaat gaatcaattc aaggcgatca tcaaactgaa ctttttcagt tgttcgtttg
    17881 gcgtactgca gccccacgcg ataatattgc tcggcttcgc tccatagctc ggttcgttgt
    17941 gcggcatcac ccaataactg attatatttt agatataccg agctatcggt aatagccata
    18001 gggtgtgttt tgatatacga aaaaatagaa tcatattgcg aagtttgata taaataggaa
    18061 accaatttat acgaaaaaat cacatcatcc tttttgtcgt aatgcactga ataccattcg
    18121 ccgactttat tttgatcaac ggcgtccagc catgaagcgt acagagtgcc gttacccgga
    18181 tgtcgcatca agtgatcgga ccataacgcc gcttcgtata cggatgtcgt ttggtcatag
    18241 agcgacataa tctcatgcgt caaatccata gctttttgga tttgaccttg gcaaaataca
    18301 atatctgctt ttaataataa agcccatcct cgcatagtat aggaaagcgt acttctattt
    18361 aatacctctt cgactttcga tagcgcggca tcatatttct tttgcttaaa atggatacgt
    18421 gcacggctca atacccagcg ttcgggcgac gtcccagttg atataatctg ttctgtaagc
    18481 ttaagtgcgc catcaaattc tttcatctca attgacgcgt aatattcttt ttgcaaatca
    18541 gcaacataat gcgggcaccg tcgtctgaat aatgaaggct gtaatagcgt ggtcgcaatg
    18601 cgaaggtcaa gcgaatcaag ttcgatcgta tttaaaaaaa acttccatcg ttcccggagc
    18661 gtgtccaatg gcagtccgta aacatcttta aagtcttccg acgcatacac gcggtgtata
    18721 ttttggatgg gatattgatc tatgagaaaa cgtacaaatg agcctgaaac ggtatatgcc
    18781 aaacccgatg aactgctcca aaaacccatc ggttccatca aggccgtaat gtccggcagt
    18841 ttgtttaatt tatagagtgc agcggaccat tcatgcggtg ttaattgcga ttcatcccac
    18901 gccgccgcta cagcgagtcc ttccagcata ccgggtttgg tagtactata aagcggatgc
    18961 ccaaacgaag acgccagtac gtgtaccgcc tcatgtttta ttgtgaattc agcatcatca
    19021 gaattaacgt ggacctcatt acgccatact ttggcgaaat ttgttccccc ggcacccatg
    19081 agtatttttt ttgtttgcgc atcgggatag atgaatattt taactttagc gggattgata
    19141 ttaagccaat ccgtgacctg ttttatcctc gcgtcgacat aacgtgttat aaatttagaa
    19201 gtcgtatcac tataaattgt gagtcgttca tcttggtaca ccacattcaa tttttgttcg
    19261 agggttgcat aggatgtgtc ccagccccag ttatcgcgcc agtaaaatat gcctgtaatg
    19321 atcagccata ccgtagttat ccaaaaaaca aatttcttgt aagtaatagt gttattcaat
    19381 tttcgtgtga ttaaatcacc ggatataaaa atggcaaatg ctaaccccaa tgagaataaa
    19441 cgatgaaata aaagcgtatc tgtcaatgga atccattcgt cgtagatcgg acctgcaaaa
    19501 tagccccacc aaatgctata aacgaaaatc ggtgcggtga aaagcaacca aatcagattt
    19561 aggatcaagg tggataacag gattgtcgcc acccacaacc aaggccttct ttggataaaa
    19621 aagcgacaga catatccaag ggtaagacct atccaacttc ctacaaaggg aagcaaaaaa
    19681 tacaaaccaa aagtacgata ccaatgcaaa tgatgaccgg caatcatcgc aatcgaaatg
    19741 atgacaagat caaaaaaaat aatcggcaac aacaaacgat taacggttcg taaaaaaccg
    19801 taacgatagt ttatatggga atgcgccctt tccgtgctca tccaaacggc gaagaaaatg
    19861 acattaatta tggttaatgc ctcagcaaat tcataatcca aatcgtatgt taagggtata
    19921 aatgccatca tcgccggcgc gatgatccgt agtattagca ttgacccgcg aagtttattg
    19981 gatagccaaa agctaaacat gtatttcctt ttaacggctc agcacggttg attcgatagc
    20041 ttgtacgatg cgcagataac gacgatcatc aagatagatc ggcgcgtaag cagaccaacg
    20101 gtgtgttcgc ttgcccatct gaatcttcaa ctcgtaaaag ctcggatatt taccctgcat
    20161 ggttttatta tcatcaagac gccagacatc gtttgcttcc aaaacctgcc aaaggtacaa
    20221 atgttttcca gcatctaatg tgtatgcact cagactatct tgatgtacac ggattatatt
    20281 attgttcgaa tagatcgaaa aatttgtttc tgtgtttttt gagtttgcat gattgcagag
    20341 tgacaattca atgcgcgagt tttcagccga caaagaaatg ccgggatacg tctcttcaaa
    20401 catcgtttct atataagcag tcagcttttt gaccgaagat tgatccgttt taccaaatag
    20461 ccgccaatca taactctttc ccgaatgcct tagattgata ttgactattt ttccgtcgga
    20521 ttgtccatac acgttggatg gttttatcat tttgtgtaaa tcattcacac actctaatag
    20581 atcagagaac tgagacggat tcatgaataa tgtatcaccg gtggcttgac gcagcgtatg
    20641 cccttcgcgg taatccaaaa tacctgattc ttttgctgtt ctggcaatat acatcaggcc
    20701 tatcaaactg tcgcggggat tggtttcaaa aacaagacgt acatggaagg catgagcggg
    20761 attgttgatc gcgtattcca accgaaaccc gtcgcgaata attttgatgt ttttatcgca
    20821 tcccaaactc gatataagcc atagaatgca aactaaaacg acgaatcggt tattggaaat
    20881 cacggttcaa acttatgtac aagctcggtc gtaaagtcag gacggacccg taccgtaatt
    20941 tcgtgatatt ttttaaatga cgggtgcgtc aatcgtactt tataaaaacc gggtttcaat
    21001 tcgcgcttga ccggcgtttt gtagtcatcg tcgcgtacgg tattaagaaa tatttcggcg
    21061 ccgggcggat cgcttaccag attgagatac ccgttcgggt ttgcaaaaac aacacgcaca
    21121 gtagccgtat cgcctttgac agcgcggaaa ttcattagcc gtttttcatt acgcaatacc
    21181 gctcgtaatt tatgcgaatt agcaagcaaa tcatttacaa taaccggtgt tttacctacc
    21241 gattcttccg atccgtctaa gtacacgtcc gcgcctgtgg gattactttc aaaacgcacg
    21301 gatgtccatt gcgctttgag ggaatccgtc agtacaagca ccgaatccgg ttccacacgc
    21361 aaagacttga ctaagtcgtc ataaccatcg gctttaattg ttagtacttg atttccgacc
    21421 ggcaagacat atgaattatt tctataattt ttctgaattc gtcttccgtt caccataaga
    21481 tagctttgtt ccggaaaaac ctgatccagt gaaacggtac cgcgttgttt ttctaatgat
    21541 tgctccacat acgtcagacg tttttttcca agtgcaatgt cgcgtttacg cgaaataaaa
    21601 tcctcacgtg aaaaaatcaa cgtatagcga cctccgggga aaatcaaact gtctatcggc
    21661 gtaaatccta aaaacttacc gtagcttgtt tccactttga ccatatccgg cgatgagcga
    21721 atagtaacaa acgaagtatc gcttcccggc ggtatttcga ccggtaacgg tttaagatgc
    21781 gcttctatgt ttttttgttc cccttttccg aaaatcaccg cggtttcgta tgattcgtaa
    21841 tgatcgcgct ttaccgtaat gcggcgttca ccgaccccca cgctatcgag acgaaacaat
    21901 gaactttgcg gggtataacg atcaccgtca atcataattt cggcatcggg tacattgaga
    21961 ttcatgatca atgaagcggg acctttttcg ctcaaagaac gcgccatccg actaaattca
    22021 aatccgccgg accatgtgat aagcagtaaa atcgtagcta atgtcgccca accgaacgat
    22081 accatgccga cgtatcggtt ttcaaaaaat cttacgattt ccgttttttt atcggcagac
    22141 ggcggtacac ttttagaaat agtcgtccgg tggccgcccg atgataattg ctttttggtc
    22201 acttccagcg ctttggcaaa ttcggttccg gattgataac gttctcccgg acccttggcg
    22261 atggcccgca tgacgatagg attgatgatc tccggtacat tgagattgac catgctgggc
    22321 ggcatgggat tctcgctgac cacgcgatac atgatctccg ttagattttt tccaggaaaa
    22381 gctttttcgc gcgtaagcag ttcgtaaaaa acggcgccta aagcaaagat gtctgtccga
    22441 tggtctaccg atttgccggt tatctgttcc ggtgacatat atccgggcgt tcctaaaatc
    22501 gtaccgatca ccgtcgtact ggacgtagtg attttagcaa tgccgaaatc catgatttta
    22561 ggaatatctt ccgatgtgag gatgatattg ccgggcttga tatcgcgatg tataatatca
    22621 tgtttatggg catagtctaa accatgcgcg agctggatca ggatatcaag aatctgctca
    22681 accgtaaggg tttcgttgtt ttcgatgacg gattccaatg tccggccatc cacatactcc
    22741 attgccatat acgatacgcc attttcttct tcggcgtcat agatggtaac tatattcgga
    22801 tgagtcagtt tacctgcaaa tttggcttcg tgcacaaaac gatccttgag ttgctggatc
    22861 atttcatcgg tcatgccttc acgaaattgc aacattttca aagccacgac acggtcaata
    22921 cgcgtatcca cggctttaaa aactttagcc atggctcctt cacccaaaag gctaagtatc
    22981 tgataccttc cgattttttc agggtaggat tcgtttgtca taggctcgtt aaaataaatt
    23041 caaaaaaact cagaattatt gattaccaaa acggttttcg atatgtgtga tgatggcctg
    23101 tatttgttca tccaaggtca tgagtgaatt gtctattacg atcgcgtcgg gaggttggcg
    23161 cagaggcgct tcggcgcgag tcatatcggc gtgatcgcgt ttctgtatat cttcgatcat
    23221 cttgggcaaa tcccattcat cggtgtccgc catttctttc aaacgtcggc gcgcacgttc
    23281 ttcgacattg gccgtcatat aaaacttgat atccgcattg gggaaaacca ccgttccgat
    23341 atcacgccca tctacgacgt aaccgccctg gttaccgata tgttgctgct gttgccgcaa
    23401 aaaagttcgc acctcggcta tcgccgaaac ggcgctgaca tggcgtgtta cttcgggtga
    23461 tcggatacct tcggacacat cccaaccatt gagaaaggtc tgctggcgtc cgtctttgta
    23521 atcaatatgc aacgtaatgt ccggcaacat cgcaatgatt ttttccggat cggtcggaaa
    23581 aataccgtga tgtatggctt gcaatgttat cgctcggtac atggcaccgg tatcaagata
    23641 acaataacca agccgcttag ctaataattt agcggtcgta ctttttccgc ttcccgcggg
    23701 accgtctaat gtgatcagta attttttcac ttcactatga ctattttttt atcaaacccg
    23761 tggctttttt tagcgccaac acctcagccg gtttgagatt acgccactgt ccgtatttga
    23821 gtttaccgag ctgtatagga ccgtactgcg agcggtgcag ctccctcact ttatagccca
    23881 gtttacgaaa catgcgcttg acttgtttgt ttttaccctc gtggatggta attttgacaa
    23941 gctcttttcc caatttttcg gctttacacg gtgctgtttt accgtcaatc agcacgacgc
    24001 cgcggcgcaa ttgctgcaac tccgattcgc cgattttttt attgagcaaa gcgatgtatg
    24061 ttttttcaat gccaaacttg ggatgagcca aacgaaaagc taattcgcca tcattggtca
    24121 atagcaaaag tccggtcgta tcaaaatcaa gtcggccgac gggatacaca cgttccgtaa
    24181 ttttgactag atccacgacc gaatcgcggc cccgctcatc tttgacggtg gttacatacc
    24241 gcaacggttt gtgcaggata atgtacacgt ctttttcttt gatcgacaga agctgaccgt
    24301 ccacttcgac acgatctagc gcctcatcca cctgataaga taaatccgta accgtttgtc
    24361 cgttgacggt gacacgccct tcgccgatga tttcatcaca actgcggcgg ctggcaaccc
    24421 ccgccgtagc cataaattta ttaagtcgca tcataattta aaattcatcc gctgcaattt
    24481 ttttcttttc gatcacgggt tccgcgatga tggcgccggt gtccatcagc gtcggtaact
    24541 ccggtttgat ttgcaaagct gttttaaaag tatcctgcca ccgtacactc aattcggaca
    24601 cctcaccggg ttcgcgaatg gctttcagat gaactaagca ctcattcact atttcttttt
    24661 gattaacagt atcaaatggc gcaccgaaat acatgccgaa aggataacgc actaataacg
    24721 tacgcggcga tttaacgttt tgcgcaatgg cttttaccgc gccgatggtt atcgtagtaa
    24781 tatcgttctc ttccagaaat cgcgcgatca ttcctgctgt ttgatgcccc aatggccccc
    24841 cgggcgttac caaaacaacg tctaccaatt gtttttgaag taatttcagt attttgggca
    24901 tacaatcttc aataaacaaa cgcgggtctc tgacttcgcc catcacgctg tagtgggttt
    24961 ctgcaagacc gccgatgcgt ttttcagaca ccatctcttg taaacgatga ataggaaaca
    25021 cgctattgat atccaccccg gcagacccga atagatccat gtaacgatgg gacacacgaa
    25081 cggtttcttg ggtaacggaa ttcggaattt cccgaaatga gacatccggt atctcatctt
    25141 caaaagaaaa cggtttttgc gttttccaca tataaagtcc agccgtagtg atgatcgcaa
    25201 tgcgcacttc ggaaagatct ttagacaaat gcttccatgg aatgtcttga ttgacgatcc
    25261 aagtctggtc atttaaataa tcaaaattca tcgcctctca cttcaaatga tcttcgggat
    25321 taaaattatt ttctttaagt atcgtatcta tttcagcgaa ttcctgagac agtccttcgt
    25381 tcttcgctgt ttcgtcagcc ggtttagccg cagcttccgc agccttttta tcggcaaaat
    25441 caaattccag cgtctctgca aattctttat cttctttgat gatgtcttcg atctctttga
    25501 gcttgggaag ttcattgatg gcatccaaac cgaaatgctc caagaacggc ttggtcgtgc
    25561 cgaaaagtaa aggttttccc ggcgcatctt cacgccccac gaccgtaatc aggttttttt
    25621 ccatcagtga acgcaatgtc caatcggcat tcacgccgcg gatacgctca atctccgcac
    25681 gtgaaatagg ttgacgatac gccacaatag ccagcacttc gagcgaagat tgggataatt
    25741 ttttctccgc ttttgatttg aaaagccgac taacccattt gtcatactgc ggatgggtgg
    25801 cgtattgata accgccggcg attttgataa ctcgcaacgg taaattgttg gcctgataat
    25861 gcttattgat ctcatcaatc acttgtcgaa ctaatgaagc ctgaacgtaa tctttttgaa
    25921 tacactccaa aagacgatcc atcggcaaag gttcggtaga tgcaaaaata atggactcaa
    25981 ctacattttg cagtggcaat ccgtccacaa tacgcggatc cgcagggacc tccgatgtct
    26041 cggtatcaat ctgtacttcc gtgggttcca agttggattc gttttgattc tgttcttcca
    26101 taatctcttt aataacctat cattttccaa aaaagcgttt gacaaaagat actattttac
    26161 gaacaaatac cgttataata gacggttttt ccgtttcatc tagcgatggt ttttcgtcgt
    26221 ttgttgttgt gcggctaaga aacgcatcgg cggtttcatg ttgttcaacc aagggtttgc
    26281 tcacatcatc ttccatttta gctgagacaa cagactcgcc atccgttctt ggcgcgtctt
    26341 gtgaaacgag cggctttact aaatccggtt gaatcgtttc ataattttga ttgtggatcg
    26401 cttcgatgat atgaacagga tcggtgttct gtggtggttc cgacgtaata ctttcaacca
    26461 catcctcgga ggttaattca ttttcgtgaa tagcttgaat atccggcgtc gaaacatcgt
    26521 ttgaccgaat taatgcgggt tcgttttcct caaccgatat tatcgtatct gatgcacttt
    26581 gatctgcgtt aatattatcg ccgatttcgt ttgaactaac ctgcggtggt gtttgatcaa
    26641 tatcagtcgt atttgtttca gcctccccga cgatcggctc attcgttggc acaatcactt
    26701 tatcttcagt gtctgataga gctaaattgc ccgtgatttc atccgccgat tcgagatcgg
    26761 aattgtttac attgacttgc gattcggcga ttactgttct gataagttcg acgggaacgg
    26821 gcgatgctat cgtttggtct tctgtcgtgg atatcaataa aacagcatca tcagttacat
    26881 ttccatcaac agtcgcgtcg gatattgagg cggttaaatc cgttgcgatc ccatcatcgg
    26941 aaacattttc tattgtacga tccaataact ccgtttcttc gtgtgaaaaa tcctcaccat
    27001 gtgaacccat attttccgtt ttaacatcgc ttatatcaga ctgattttgc tcggcagaag
    27061 taacttccga tggtacttgg gtataaaaat caacttccgt tgattcattc accgatacag
    27121 atattgtttc atctgaagac gaatttgacc gtatttcttc aactgaataa atgttttcat
    27181 ctgaaagaga atttaaattt tcttcgactg tagcgcctat aatttcatca ttttcggaat
    27241 cagcaaacgc ctcagatgta tcagtatatt ctgtttgcgg ttgttcttcg gtttgtggag
    27301 ccgaaacgaa ggcttcgtta tttaggattt cttcatttaa ctcaaccgtt tccgcatcgg
    27361 ttttcgctgc ttcgataaca ctctcaatac tcatttcaac tgcggcttgt tcgacttctt
    27421 gcaccacgcc atcggtttgc gccatatcaa tgtggtcgtt tgctaattcg ctatcgggcg
    27481 atgcggcgac ctcaattaat gacgtatcca aagccgtgtc tccggattca ttaatcgcaa
    27541 acgttgtatc gtttgatgaa acttcattca acacaggcgc ctcggacggc gtgagaacag
    27601 gttcatcgtt tgaaggtatc gtcttttccg tttcggattc ttcagcgcgc aactcatcgg
    27661 gaatttcatc cgtatatgca gatgctttat aaatccaaat atcatcaaaa agcgtggttt
    27721 ggcgtgctgc gatttctcct tttttaacga gatccagcaa tgctaaaaat gtgaccacca
    27781 cctcaatctt gctcatatcc tcgacgagtt ccaaaaacgt gtacatattg cgatcacgga
    27841 aaaaatccat gatgtattcc tgctgttctt cgaccgtaac attgaggcgt tccacgttgt
    27901 gataaatgac agccggcttt ttggccattg catttttata agccgtcaaa agatcaaaaa
    27961 aggagatatc ttttaaacca accgcttctt cggtttgctg agggatcaga tcaaaattga
    28021 aatagctccg gcgatatacg gagcgccaat actcatccag ctcggccaat gatttggaag
    28081 cttctttaaa ctgacggtat tcgacaagcc gacgtgacaa ttcctgacgc ggatcgataa
    28141 gttcctcttc ttcttcggtc gtcggatccg gcggtaacat cattttggcc ttgatacgca
    28201 tcaacgtggc ggccatcaga ataaactcac ccgcaatatc caaattgagc gtacgcatca
    28261 aatgcaggta ttcgagatac tgcttggtga tgtatgcgat aggaatatcg taaatattca
    28321 gttcgtcctt acgaacgaag aataacaaca aatccaaagg gccttcaaat tgttctaact
    28381 ttacacgata cacgatgcgc cctcaattat tatccaacgg tacattacca acaccctttt
    28441 tcttcataag tttttcgatg tcttctattt tgcgaggtac acgctcagac agattgatat
    28501 tgccgctttc cgtgaccaaa atatcatctt caatgcggat accgatattc caccattttt
    28561 tatcgcacgg tgcattttca ggaatataaa tacccggttc taccgtcgta aaaaaacccg
    28621 gaacgaacgg cttgtcatac gactgcggat catgcacgtc cagtccgatc gaatgcatat
    28681 agccgtgaac ggtatatttt tttgcttctt tgcgatcctg aatcaatccg atttccaaaa
    28741 gaccgtcttc caatgtttgg gtaatggcat ccgctacttc acggtaaggt acattcgttt
    28801 taatgcggtc aatacccgcc tgttgagctt tgagcacgat attgtaaatg atggtttgtt
    28861 ctttggtaaa tttgccgttg accggatacg aacgcgtcac gtctgcggaa taaccgtggt
    28921 actcggaagc catatctacg agcaatacgt caccgtcttt catttgacga cgatcggtcg
    28981 tatagtgaag aatgatactg ttttcggccg aaccgcagat actgccgtat ccttcgtatt
    29041 ccgcgcccag gcgcgtaaag acatattcac cgaccgcctt gatttcaaac tctttttgcc
    29101 ccggttcgca gcttttcatc atttgcaaat gggcgtcgtt ggttatctca atggattttt
    29161 taagtaagac gatttcctcg tctgttttta cggcacgcag ttcgccgatc aacggttcca
    29221 cattgataaa atccaccgaa ttcatatacc ccaaaaggct tcggggatta ttacgaaaat
    29281 ccgtaaacgc cgtcgtcatg ttacgcagcg tcttagtgtc atattgctca cgtaaattaa
    29341 aacaatacac attttccaat ttcgaagcgg caagctgttg gagtaaaatt ttgtttaact
    29401 gcgtactgaa ttcatcattg gtaaacgccg tatcaatgcc gagaattttc tgtgtgccgt
    29461 cggtacccat acgacgtccg ttatatactt catagcggta atctcgtttg ggaacaaaca
    29521 taaccgtcga atatgttttt ccgccgacaa cccaaccctc cggcaaataa atcaatgccg
    29581 cattgggttc acggaaaccg gtcagataat agaaattgct ctcgggttta tattgataat
    29641 acgtatcgtt gctgcgaagg cgttcttccg acgaaaaaag cacggcgatg ctgtggggtt
    29701 tcattttctg agccaacgca tcgcgacgtc ctttgtgaaa ctgcggcgaa agccaatcgt
    29761 tgtcgtaatc tttgtacggt tggatctgat cctgcgcaca caacgtactt atgctgagta
    29821 tccaaaacaa aacgtaacgc atagtttttt cctttcgtgc gttaatcaag tttcatagct
    29881 cggcgaaccg aaaccatcgt atcggacgcg atagcgcggg ctttgtcgga gccctcggat
    29941 attatttttt cgaccgcatg gagatcggat accaattcgg cgcgtttttg gcgcataggt
    30001 tctaaacgtt gagatagttt ttgtccaaga ttttttttgc atgcgacgca ccccaattcg
    30061 ccggttgtac atcccgcggc gataccctgg gtttcgtcag tattaaacac tttgtgatag
    30121 ctataaaccg aacaaacctc cggtcggccg ggatcatttt tacgcagctt ttgaggatcc
    30181 gtgaccattt ttcgcacctg cgattcgatc gttgcgtcat catcggaaag ataaatacaa
    30241 tttccgaggc ttttgctcat tttggcgtta ccgtcaaccc ccggaagtcg cggtgtga
//
LOCUS       DAOQGF010000033        30291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_678_length_30291_cov_6.801660, whole genome shotgun sequence.
ACCESSION   DAOQGF010000033 DAOQGF010000000
VERSION     DAOQGF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_30291_cov_6.801660"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..1191
                     /locus_tag="PK937_06585"
     CDS             544..1191
                     /locus_tag="PK937_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH32272.1"
                     /translation="MKNKKITVWVVEDNDAMRTTYAELINSASNMRCIGNFDAAEEAI
                     KALSAQEHPDVIMMDIELPGINGIEAVRRIKEKAPAINIIMQTVYEDNEKIFQSICAG
                     ATGYMLKRTPAEKMIESIYEAHTGGAPINAQIARKVLTMFAQEMRPSGADYNLTVREK
                     EILQNLVSGLTIKMIADKLSISAMTVGTHVKKIYAKLQVSSRGEVVAKAIQEKLV"
     gene            1322..1867
                     /locus_tag="PK937_06590"
     CDS             1322..1867
                     /locus_tag="PK937_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32273.1"
                     /translation="MCPTDDKENASKIPFNGARWADSVVGMSSEYYPAVASWNAVEAL
                     GEPNVYPDYGDIPYAWTSQTQDGQREYLELGFFSNPQPVASIAIFETYNPGAIDTLYV
                     MNPNNNLWEIVWSDSAHDRGDSSRIFIANFAKTSFNVSVIRIAINSPKVISWNEIDAV
                     AMAQEEIPPFYDDSTGFYSKD"
     gene            complement(1864..3822)
                     /locus_tag="PK937_06595"
     CDS             complement(1864..3822)
                     /locus_tag="PK937_06595"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNH32274.1"
                     /translation="MPNSSNNSDSSTVSVSPVIIATTAITSFTITFALYLNTLAPTVT
                     FEDSGELIAAAAHLGIPHQPGYPLWTLLSHLFTWLPFGNIAYRVNMASAFFSASGAAL
                     IAWTFLLWLRLTYIDILKKSSLGFLFSVLTAFLTATSSEAWEQSVISEVYGLHIFFTG
                     IILLLTTLWLSETGNNERSRYFFLLSFVLGLSLSNHTTTLVLLPPLVMLAFITDRNFI
                     LNLKRIIIATGFITLGLSCFIYLPLASARNPMMDWGNPETWTNFWRVIARHQYQLNET
                     QTLVKFISQLKTYFSLLGEQWFSPILVLVLPALVFLWKRHRSLLLFVLVFWFFTAPVT
                     TYLTNFDVTTGNPLVSAENKALVSVFYIPSYMIVAALVALGTISLVEQWPSRVTYIAA
                     GLLAIACLWHLPENYKRNNMHQYRFTECFAENIFSVTDSNALVIGNWDPLVFPFFYYQ
                     QVEKRRPDLFMIDQELLRRSWYIQMLRDHNPESTELIKKEIDDFLDAVKPFEAGEKFD
                     GNYIQRRYERMINALIDNWMQSGRSVYLTYDPPKGIAEKYYKESVLTAIRLRTDMIRP
                     TSLDHKNIQTEIFTDEAINKDRMARFFVTYYGRLFYARAAIMESLNQKENALTLYERA
                     AQLLKNQSSYTPTLENAIRRLQAKAATP"
     gene            complement(3936..6398)
                     /locus_tag="PK937_06600"
     CDS             complement(3936..6398)
                     /locus_tag="PK937_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32275.1"
                     /translation="MQNGRHAEGFILNMPGAIYTNFSHDAIERSFITKPILPVIDPES
                     QSGVIHIRSDFSDALGYRVNGFPTTDVFDSRHHIQLPHRAIASVLLQEGGSDTRTGNH
                     VRTADIQTKQGGTSWFAAGSIFSDMPAKLLRLPSYGKHVFDGEVSGPIIPQKLYMYSA
                     AEYESFDDADPSVVGVPRYTIAKGSRLYDHDNNNATASIPIDTVLWGNIKNGARPNGS
                     NALRRLNAYARFTAPLDQNLNLEFSGTFSQMKRNWFNMFYLLSPYGVPRSENTAWDIQ
                     SSVHWRVDQQLSFSGGLRYYVNKETITNRKLSSSDSYALRHLQDSYRGRTGFLTYMSD
                     NLFYDVGAGYGMFIRKESSTASVSVSSQYMFSNTLFIESGVSYTSGIYRSATIYDSEN
                     LQFGSNDIFGYSITDDFKLKRKNSGLDGVKKPTLINAYGMGNFKMKNFSGTFGLHVQR
                     LTSGTRELRNLHDPSGMYNDVSPGVLDEKDFYSHEYTTILFLPRLGVNIQLNEMLSAY
                     SHYGWYSTPGGLQRRYVGSEFLEKTVLAPSVETIIGNSKLKPVESELFDLGARVNFSS
                     PSSMGAQFYIQEIKNIVDYTKVVAEPNGYISYYNGPKAGIVGFAADITLIPHENFFII
                     WNYGAQTSEFELGSNVSSYHFAWLGFNTANFAVPVSHVRTWQFGQQATLHFDNSAAEP
                     LLKGTFASMEWHFKDGSFYTPTTMAPIQAVGYNNQYPVSKKNSSETGSLWTIDIKAGK
                     SFVWKNHTATLFIECTNLYNNKNPTQVFSSSGKPDDNGFLDSPYFLDYQKRIRPQYKA
                     HFRNGLMYEPGRELRMGIEISM"
     gene            complement(6302..6823)
                     /locus_tag="PK937_06605"
     CDS             complement(6302..6823)
                     /locus_tag="PK937_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNH32276.1"
                     /translation="MSLLHFLRAAFFSAVFSSVAFAGDFGELIVRITTKDGSPVAGSR
                     IALAQTPYSAVTNEEGTVRFYLLQSGVYALHLADETDTSAEPVNVYISGDYPTEIDLT
                     LENGKLEIYRGQSNPETMVQKYFSHTVRLKTAQTQPKLRLCKTDVMPRDLFSICRVRF
                     IPTFLTMRLNARS"
     gene            7134..8093
                     /locus_tag="PK937_06610"
     CDS             7134..8093
                     /locus_tag="PK937_06610"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNH32277.1"
                     /translation="MTITGYYSILAAIRKLWINNCLIPHFEKVRHGMKEILKSYLTLT
                     KPTIMLLVVFTGATALIVEGSFLHEPVKFILVLVGLYLTGGCANGLNQFFERDIDAKM
                     ERTKSRRPLPGGILSPMHALIFCSIIGVSGVILFAVTFNWLTAILSLATILFYSFFYT
                     LYLKPNTSQNIVIGGAAGAMAPVGAWAAATNTMAPAPWIMFLIVFFWTPPHFWALALF
                     CKNDYIKAKLPMMPVVKGDDSTLRQMFYYSLVLVVTTLALIFFKSGWIYAVTAIILGF
                     FFVKKAYDAMKYKTEKIIRGLFGYSIVYLFALFLAMIVDSLVA"
     gene            8274..8930
                     /locus_tag="PK937_06615"
     CDS             8274..8930
                     /locus_tag="PK937_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH32278.1"
                     /translation="MAQIFPKRANKTPILAAAALGFLGMAVTGFFWYYGSPKFTDVGY
                     RPVQPVPYSHKLHAGDLGMDCRYCHVGVETAAAAMVPPTATCMNCHKLVLPQSEKLLP
                     VRESFANNTPIQWVKVHKIPDYAYFNHSIHVNRGVGCESCHGNIAQEEVVQQRQPLSM
                     SWCLDCHRNPDQHLRDLQSGVKVTDMNWTPPADQAKFAARIKQEKKIAPPEDCSGCHR
                     "
     gene            8927..12052
                     /locus_tag="PK937_06620"
     CDS             8927..12052
                     /locus_tag="PK937_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNH32279.1"
                     /translation="MSQKTTLTQNGKEYWRSLDQLADTPEFKEFVQREFPEQASEMSN
                     PVTRRTFLSLMGASIALAGLAGCRRPVEKIIPYVIKPEEIIPGVAEYFATVMPFGTGA
                     YGVVAKSHEGRPTKVEGNEKHPSSLGATNAYATASVLNLYDPDRSAYVLNKGNQKLWN
                     DFVAAWRELFAKYTANKGEGLAIVSESFASPTMARLQAEFTKAFPKATWTSYEAVSDE
                     NIYAGIEGVTGNKLQPVYNYGKAGVILSLDADFLHMESDNITASRGFADGRRMKDEND
                     TMNRLYVVESMFTLTGGMADHRLRLQSRQIGAFTVALAKALGVDTMGINADTSVLDEK
                     ANAWIAAVAKDLTNNKSKSLIVAGRRQPAAVHALVLAINSALGNVGTTVQYHAAKDMM
                     VSDRSGLAELTKSLSDGKVSTLVMLGGNPVYNAPADLKFAEAMKKAEHTIHFNGYVDE
                     TGSNGVEWHIPASHFLEAWGDVRSANGTVSAIQPLIDPLYATHSDIEVLYLLTTGKEA
                     RGYDIVRETWKTLFAKGLDFEKAWRLALHDGVVGDTATAPVTVKMDAKAASAFVAKNA
                     LPSGSADANNLEIVFYASSATFDGRFANNGWLQELPDPVTKITWDNAAIMSHKTAEAL
                     KVKNILGEGKNHQPMIRLKYQGQTLELPVWVQPGHADYSIAVALGYGRQSAGRVGNGV
                     GADTYALRTSAAMDFDLGLTAEKLSKTYLIATVQDHGGFDTDALADNEIQRRIPTIIR
                     EATIEEYKKDPNFITSGRTPGEKELYGKDGKPISIMNMHEYGPERFEKGEWNKKYQWG
                     MSIDLNACTGCNACMIACQSENNIPVVGKEEVAKGREMHWIRLDRYYIGKDVNEPEMQ
                     FQPIACQHCETAPCEQVCPVAATVHDSEGLNVMVYNRCVGTRYCANNCPYKVRRFNFL
                     EFHAGTSGFMESKPIESLKLGKNPDVTVRMRGVMEKCTYCVQRISGARIAAKKEDRDI
                     RDGEVVTACQQSCPTGAIVFGNINDPESKVSKMKAMNRKYEVLAELNVRPRTSFLAKL
                     KNPNPELTKNA"
     gene            12210..13451
                     /gene="nrfD"
                     /locus_tag="PK937_06625"
     CDS             12210..13451
                     /gene="nrfD"
                     /locus_tag="PK937_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNH32280.1"
                     /translation="MASSGFLLLLIAITYLLFEGTGIWGITNPVGWGLAIVNFVFWVG
                     IGHAGTLISAILFLFRQKWRTSINRFAEAMTIFAVICALLFPGIHVGRMWATYWFAPV
                     PNQMGMWPNFRSPLLWDFFAVGTYFTVSLIFWYVGLIPDLASLRDRAKTRVRQVVLGF
                     LSLGWRGGNKQWKHYEMAYLILAGISTPLVLSVHSIVSTDFATSIIPGWHTTIFPPYF
                     VAGAIFSGFAMVMTLAIVARKAYKLENIITLYHLENMNKIMLLTGMMVGYAYSCEFFI
                     AWYSGNMYERFAFINRAFGPYWWSYFSMIFCNVVVPQIFWSKKLRRNITVMFIASIFI
                     NIGMWFERFVIIVTLSRDYLPSSWDYYSPTMWDWFTFLGSFGLFFTLFLLFLRFVPMI
                     AMAEVKGVLPQADPHHHNGKH"
     gene            13465..14016
                     /locus_tag="PK937_06630"
     CDS             13465..14016
                     /locus_tag="PK937_06630"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNH32281.1"
                     /translation="MSASKEKQTDEVAIVLAEFDGPATLIEAAKKMRDAGYKAFDCHS
                     PFPVHGMDQAMGLARSPLAKICFVIAMSAVLGMAYFTYWLSFDYKLVISGKPLFSYVA
                     YVPPVYAVGILTCALTAVVGMMVLNGLPQPYHPLFNSDNFARVTQDGFFVSVESTDPQ
                     YDEAKVKSLLASLGGTNVEVVKG"
     gene            14042..14650
                     /locus_tag="PK937_06635"
     CDS             14042..14650
                     /locus_tag="PK937_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH32282.1"
                     /translation="MKSSKIWSTTVIVAVMALSACQGRPSEEPPIHLNPNMDSQPKYK
                     AQSKGDFFADGMSMRTPPEGTVARGQLREDAAYYMGRDEKGNLLKENPVPVTMALLHR
                     GQERFNIYCTPCHGQTGLGNGIVVQRGMLPPPNFHDERLLKVEDGHFFDVMTNGIRNM
                     SSYKSQVPVADRWAITAYIRALQRTHTASLEDVPQEKRKDLK"
     gene            14655..15848
                     /locus_tag="PK937_06640"
     CDS             14655..15848
                     /locus_tag="PK937_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32283.1"
                     /translation="MKLNTETFRLEDAGSFGKKALMAGIVGLLISVAGYFVNADQFWR
                     SYLTAFTFWTSIGLGGMFFVLVKHLAGAEWSVVLRRLAENFMITLPIMFVFFLPILFG
                     SHHLYEWTHTEVVAKDPILSAKSGYLNMTFFTIRTVLYFAIWTIFGRMLYKMSIGQDG
                     GDQSKNNRMRQLSAPGMILFAFSITYASFDWIMSLQPHWFSTIFGVWYFAGSLLNFLA
                     MLVLIAIYLRKRGVLDNVISIDHFHDLGKLQFAFTIFWAYIAFSQFFLIWYANVPEET
                     IFFLKRWEGSWMYVSIGLLVCHLMIPFILLIPRANKRNLTIMKFVAIWFLFMHWVDLY
                     WNIFPTWHDAHHGVVMSGLWVDAATILGIGGIFLWYFWMLLGKQALAAHKDPSFEKSM
                     HHVNL"
     gene            15865..16230
                     /locus_tag="PK937_06645"
     CDS             15865..16230
                     /locus_tag="PK937_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32284.1"
                     /translation="MSENTQEQIHPEDGYEKSDVNARAILVSTLIGIILIVASLVLVD
                     EYFIQGREQFYTEAVLKPESAPLRDLRTKEAEFLNGYAVIDAKKGVYQIPITVAMERL
                     AAEAFEKTQTKKDGKISKK"
     gene            16253..17071
                     /locus_tag="PK937_06650"
     CDS             16253..17071
                     /locus_tag="PK937_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH32285.1"
                     /translation="MRLRSLLCITVLLQYVCSTLDAQVILEKNQDPELQKIDVIEHLG
                     DTVPMNLQFKDEHGAIVQLSDYFEKGKPIVLTMAYYRCPMLCTMVLNAKAEAVKQLAW
                     LPGREFQMLTVSIDPNETPELASNKKDTYLKFLDKSGIQEGWHFLVGDSSQSKALADA
                     LGFKYFYDENRKEYAHPAVVFVITEKGIVSRYLYGIRFAERDMRLALMEASEGKIGST
                     IDRIIMYCFHYDPNAKGYVVMATNVMKLGGLVTLVVLGVFLTVMWRRDKTRSIQ"
     gene            17111..18040
                     /gene="coxB"
                     /locus_tag="PK937_06655"
     CDS             17111..18040
                     /gene="coxB"
                     /locus_tag="PK937_06655"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNH32286.1"
                     /translation="MNDFWMPPAHSTVAHEIDSLFYFIYYCSVFFFVAVVGAMTYFAF
                     KYKRKAHEMTEPAAHNNTLEITWTVIPTILCLVVFVLGFRGYMKMNVVPKDALEIKAT
                     GQKWFWSFDYPSGATTVNDIVIPVGKPVKLLMSSKDVIHSFYVAEFRTKRDVLPNRYS
                     ITWFEATQADTFLIQCTEYCGTSHSEMIGKVIAVSETDYNKWLEANSNPGEGLTPEAY
                     GEKLYTSKACITCHSLDGKPGSGPSWKAIFGKAEKMTDGSTVTIDENYLRESILNPQA
                     KIVAGYQPVMPTYQGILKDREIDAIIAYIKTLK"
     gene            18053..19726
                     /gene="ctaD"
                     /locus_tag="PK937_06660"
     CDS             18053..19726
                     /gene="ctaD"
                     /locus_tag="PK937_06660"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HNH32287.1"
                     /translation="MSATVTEKNVSSASTGMPEHHFLNSPQGLKSWLLTLDHKRIGVM
                     YLFGIMTMFFIGGVFALLVRLELMSPGEDIMGKDAYNKMFTLHGAVMVFLFIVPSIPA
                     IIGNIILPLQIGAKDVAFPRLNLASWYVYLAGSMFAIYSMITGGVDTGWTFYTPYSNT
                     SNTSVISMTTAVLILGFSSIFTGLNFIVTVHKLRAPGMTWFKMPLMVWGIYATALIQV
                     LATPVLAITVVLLMLEKMWHIGIFDPAMGGDPVLFQHFFWFYSHPAVYIMILPGMAII
                     SELVAAFSHKNMFGYRFVAFSSLAIALLSFLVWGHHMFVSGQSELSAVIFSFLTFFVG
                     VPSGIKIFNWLTTMYKGSISFSAPMLWTFSFLFLFTIGGLTGIFLGTLSVDIHLHDTY
                     FVVAHFHYVMMGGTVMALMGGLHYWWPKIWGRMYNEFWARISVLLVFIGFNMTFFTQF
                     VMGSRGMPRRYWTYQDQFTSLHGFSSIGAFVIGAGFLIMAFYLIHSIFKGKPAGNNPW
                     GALTLEWTTSSPPPHHNFDQTPIAVNGPYDYDKVKAEITYEPNVPKAKV"
     gene            19751..20404
                     /locus_tag="PK937_06665"
     CDS             19751..20404
                     /locus_tag="PK937_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNH32288.1"
                     /translation="MSSQHSAAHPAHLAHHFSDVEQQKESAKLGMWFFLLTEVLTFGG
                     LFCAYAIYRAWYPEMFSIAHQQLNVTMGTINTIVLITSSLTMALAIRNMQLNDKKGTL
                     IMLLLTFVFAGTFMVIKYFEYTHKIHLGQLPGKFYTFDGINASNPHIFFSIYFAMTGL
                     HGIHVMIGMILIAWLMYKTSKGAFSSEYYTPIEMTGLFWHLVDLIWIFLFPLFYLIG"
     gene            20409..20798
                     /locus_tag="PK937_06670"
     CDS             20409..20798
                     /locus_tag="PK937_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNH32289.1"
                     /translation="MSNEQHKEHIIPLSTYLGVFSALIALTALTVWIAQFHFGEWNLI
                     VAMSVAVVKALLVAFIFMHLLYDNKLYATVFAGSIVFLAVFIVFTMYDTLRRDDIYEQ
                     VGKPIKSKAIIYDNMPVKKDSTAHSGH"
     gene            20823..21770
                     /locus_tag="PK937_06675"
     CDS             20823..21770
                     /locus_tag="PK937_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNH32290.1"
                     /translation="MNRFRQFALFSTIATYVLIFVGGLVRVSGAGLGCPDWPQCFGHW
                     FPPTSVDQIPPHIDPSLFNMTLAWIEYGNRIVGVTIGFLIAGTAWLAIKHYRHDKRIL
                     TASVAAAILVAFQGWQGSRVVKSQLNPHIISIHFALALLIISLMVYVTFRAYKPEQEI
                     KNISDAVYRSVFGWIKIMWVVNLLQILLGTEVRGALEVLANAFPLMSEAELMRQSNYV
                     SYIHGVVGVIIVGATWLFHEQIQRVRTQIPAYIVTISNIGLLLMIIQLFIGVLLVTMD
                     RPPLLQIVHLWISSFQIGLLVIFYAAWRRDNQSSERKTV"
     gene            21767..22357
                     /locus_tag="PK937_06680"
     CDS             21767..22357
                     /locus_tag="PK937_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNH32291.1"
                     /translation="MNTNLIMRLSIGFFVLMLISAIVIYKAKESQSDLPVYAEVTPFA
                     MTERSGEAFTNEKFKGHITVVNFFFATCAGPCPAMNARVAELYRAFAGNNPVRFVSVT
                     IDPDRDSLVALRNYAMSFNVNDNRWSFLRGDLKSVEHLSENIFMVGSGAPTLHSTKLI
                     LVDHLGRIRGYYSSEEPASLTILKTHIRELVREMPS"
     gene            22354..22767
                     /locus_tag="PK937_06685"
     CDS             22354..22767
                     /locus_tag="PK937_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNH32292.1"
                     /translation="MNAADLPFINAILNSVSTVFLTLGYIAIRKKDRVQHKRYMVTAL
                     ISSALFLTCYLIYHYQVGSVPYPYYDWTRPVYFVILVPHIILAAIMSPFIISAVHYAW
                     IGEFAKHKRLMRWVWPVWMFVSVSGVVIYLMLYRF"
     gene            complement(22753..24357)
                     /locus_tag="PK937_06690"
     CDS             complement(22753..24357)
                     /locus_tag="PK937_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32293.1"
                     /translation="MSTAPLQEERRSNGEIRSLVNILAQLAVESNKSSWHCIRETYAD
                     LLHLFHQNHEVRVSAPLLRTFEKNRRTLETVYNPTSVTGTHKQGPLREYWNKIIQMRP
                     KTNEKPVRKETDETVRGNYAQFLRQNVISLIEDQRILVLLLVGLLSRRLHHRSTSDIR
                     KTIQKFESDLKSHHARSDRAMSILIYSLCQTAMHTSLAADFAQGSLLCERAIELFRLL
                     PDYPRLRNELHLQLQEALRVSTERVVNRLQEKVLDEEIEIIEDLASKLSNPELVAYVM
                     PQLVFFLLRGQRYEECLSRMDGVLQTVTENKEFVDYISAITIGHYWCDALLKAERYSR
                     GEVNFSFIEREKDNFARVFDVVKTARVPDLGDETLYNVEIEKLAVSLSHLGTLYFQTG
                     NIPQASACYALSFENILLKKCSRNRDSVDEIMDRYKDTLFHPLNTAPPEAHPLIAMLA
                     AEKGFDFLRDTQAEKMKNEGKDAGEPIYYDDLINAQKELIADLMDATKNKIKHPFSAK
                     FSSYKPFVESEFARIVGKMKINLEAI"
     gene            complement(24447..24731)
                     /locus_tag="PK937_06695"
     CDS             complement(24447..24731)
                     /locus_tag="PK937_06695"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012195045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNH32294.1"
                     /translation="MSRKKLETYEIENRLKTLPDWKLRDGKLYREFIFSDFVEAFRFM
                     TAAALIAEKLNHHPDWSNVYNRVHITLYTHDVGGITELDFILADKLNTLL"
     gene            complement(24734..25345)
                     /locus_tag="PK937_06700"
     CDS             complement(24734..25345)
                     /locus_tag="PK937_06700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNH32295.1"
                     /translation="MTDAPFTLPTLNYDYKALEPHIDAQTMEIHHSRHHKAYVDKLND
                     AVIQAKIDKPDLDTLMRHISKYSTAIRNNGGGHWNHSMFWQIMKPGSGGAPSGELAAA
                     INTAFGSFDEFKTKFNATAMSRFGSGWAWLVVRDGKLEIGSTPNQDNPLMDVSDFKGT
                     PLLALDVWEHAYYLKYQNKRADYVQAWWNLIHWDEVTRRFKKL"
     gene            complement(25473..26339)
                     /locus_tag="PK937_06705"
     CDS             complement(25473..26339)
                     /locus_tag="PK937_06705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH32296.1"
                     /translation="MIRFALASRIKLLACKTWFMQIASVNKFPYTTILITGASSGIGE
                     AIARALAKPGFRLILVARSADALETHAREFRSSGATVDVIAMDLTSENAPQRLFDQIN
                     KLNIRVDLLVNNAGFGSYGYFHESDIAKQMNMIDLNIKTLVQLTHLFLPGMLSQNKGA
                     ILNVSSTASFQPVPFMATYGATKAFVTHFSVAVASELRDTPIRIISLCPGRTKTNFQL
                     SAGSNKIRIRSRSASKESVAEVALDAVVNYKKIAIEGLGNKYMTHLQRLFPRFVILGL
                     ARRIFEPKNSNT"
     gene            26373..27215
                     /locus_tag="PK937_06710"
     CDS             26373..27215
                     /locus_tag="PK937_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32297.1"
                     /translation="MRFVVFLTTVFVFLNRPSLASFDTKKGARPLAMGGAYTAAAFGG
                     DAFYYNPAGLWYTANPMGQAFFSRPFNLTELQTVTFNFTYPSFFGNVSANAESYGYDL
                     YRETTLSLAYANVYKERVAMGVVINYNHLAIKNAGSAAAVGLDFGILVRPLRMLTVGA
                     MVRNINHPSIKKDALPQTFTVGACFQIMDHFRLMADAYKDVRYPTDIRVGGEYMFMEK
                     FIIRSGLGSDPSRFCGGFGLDLGLAVLDYGFNTHQELGMTHALSLSFHIDRKPSRSLQ
                     PSLR"
     gene            27222..29243
                     /locus_tag="PK937_06715"
     CDS             27222..29243
                     /locus_tag="PK937_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH32298.1"
                     /translation="MVVLLQRISFFIALVFYSSVVVAQDSLWIDNDQDRSTESALESM
                     TETANEGVEFSEALENWRRNPLDINRADIEELQQLPGLSPILAKAIIDRRRAQPFTSI
                     RQLLQVDGFSRELFVRIQPFLIVYSHTAVRPFDVALRQRTERQIEKTPEFSDGSYEGG
                     PLKMYERAVAVYAPGWSALPDAQIRGAWVMEKDPGETRWNDHQAGYAEIKNAGVVRRA
                     VVGNYRLEFGQGVALWSGSGMSKSTEVIQSVKRRSGGIKPFASATENQALQGIAAELQ
                     FEDVNFLHALKLTGFYSYAHFDASRNPDGTTGGLDESGLHRQTSENRRRDYLLETLGG
                     LHADYQINSATRLGATYYAHQFNRRFVPNDPVRNRYDFEGHRNRVGSISYDYYYEHVN
                     FFGEWAMDGGADKAGNAGVQFFWPHMEFVFFYRNYAKNFHSLRGYAFGEQNGRTRNEE
                     GIYTGWKYEWRRRLTIQAYYDLYRFPWRTYDVPTAVTGRDFLTQADFALTRAMTITML
                     YKREEKDDAIQSTDILGRDIIIVEPSRVQRLRIQMEMLATPQFRFRSRIETSYYAIPS
                     MSGTSEKGLLIYEDVRFQPDRRWTLYGRVSVFDADSKSAVYEYENDVEGAFANTRFSG
                     KGLRWYVFTQYHGLNRMDIGFKYWQMARSDISGYGRMTRRVTLSVDYKL"
     gene            29370..29996
                     /locus_tag="PK937_06720"
     CDS             29370..29996
                     /locus_tag="PK937_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNH32299.1"
                     /translation="MEEKTLVGYFTDGGFFMWPILICFIGGLAICIERLITLTRAATN
                     TKQFLVKIKQSLDEGGVERATEVCASQRGSVALIFHAGLLRARKGVEHVEKAIVNAGA
                     VEMAFLERGMVWLGFFVGAAPLLGFTGTVQGMIEAFDAIAKANDISPTIVADGIRTAL
                     YTTLFGLMVAILLQFFFNYFTAKINRIVADMEESSSELVDYLLENEVR"
     gene            30015..>30291
                     /locus_tag="PK937_06725"
     CDS             30015..>30291
                     /locus_tag="PK937_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH32300.1"
                     /translation="MFVEKRRKYTPEISTASMADIAFLLIIFFIVITKFGTDKGIDLT
                     LPPEGSIKEIPKNNIMSITMDDAGAVVLDGKAVILGQVQSLVKTIAEA"
BASE COUNT         7914 a         6575 c         7294 g         8508 t
ORIGIN      
        1 tgtatagtta agatacaggc tttggatcca tcctttacgg cggcgtccaa tcagctcggt
       61 atagcgaccc tcttgctcaa taagctggag gccttggcta aactctactg atcgcttcac
      121 acaaaacctt tctactacac aaccccgttg atgagacggg gttgtgtgct tttgtctgag
      181 gcaaaacaga aagcaatcgt atgttgcaat tgttgctgga atactatgat cgcatttttc
      241 tacgattcgt tttttaatta cgttagatga taaatcattt gcagagtgat aatattgaaa
      301 aaatcaacgt gcgaatgcca gtaacgggca tcaatgagtc gaaagattta ttgatgcaat
      361 agtttaaaga gcataagtac atttctatct tagaataaac aattggctta aaatattgtt
      421 gaaaatcggt cataagagca tttaacaaac agatctatgt aaatactata caatgatgtg
      481 ttattagata ttatttgcac tatattcatg taatacgata gtgactgagt ggatacgcag
      541 aaaatgaaaa ataaaaaaat cacagtttgg gtagttgaag ataatgatgc catgcgaact
      601 acgtatgcag agcttatcaa ttcggcatcc aatatgcggt gcatcggaaa ttttgacgcg
      661 gccgaagaag cgatcaaagc attgtcggcg caagaacatc cggatgtgat catgatggat
      721 atcgaattgc cgggtatcaa cggcatcgaa gcggtacgcc gtatcaaaga aaaggcgccg
      781 gcaattaata tcatcatgca aacggtttat gaagataatg agaaaatttt tcaatccatc
      841 tgtgcaggcg ctacgggata tatgctcaaa cgcacgccgg ctgaaaagat gatcgaaagt
      901 atctacgaag cacataccgg cggtgctcca atcaacgccc aaatcgcgcg caaagtacta
      961 accatgtttg ctcaggaaat gcgcccatcc ggtgcggatt ataatctcac agttcgtgaa
     1021 aaagaaattc tccagaacct agtatccgga cttaccatca aaatgatcgc cgataaactt
     1081 tcgatctcgg ccatgaccgt aggcacacac gtcaaaaaaa tttacgccaa actgcaagta
     1141 agctcccgcg gtgaagttgt agctaaagca attcaggaaa aattagttta atccgatacg
     1201 cgttatccca tgcttatggg attgaagccc caaggtctaa tgactaattt tcataaaaac
     1261 aggaggcggt catgcgtacg tttttaatct ttgctattat attcacggct atgttttcat
     1321 catgtgtccg actgatgata aagaaaacgc atcgaagatt ccttttaatg gtgcacgatg
     1381 ggcggattct gttgtcggaa tgagttcaga atattatccg gccgtagctt catggaatgc
     1441 cgtcgaagca ttgggagaac ctaatgtata tccggattac ggtgatattc cgtatgcttg
     1501 gacttcgcaa acgcaagacg gtcaacgtga atatttggaa ctcggttttt tctccaatcc
     1561 gcaacctgta gcttctattg cgattttcga aacttataat ccgggcgcga ttgacacact
     1621 gtatgttatg aaccccaata acaatctttg ggaaatagtc tggagtgata gcgcacatga
     1681 ccgtggcgat tcgagccgaa tttttatcgc aaactttgcg aaaacctcat ttaatgtttc
     1741 agtaattcgt atcgccatca attcaccgaa agtaatcagt tggaatgaaa ttgatgccgt
     1801 cgcgatggcg caggaagaaa ttcctccgtt ctacgacgac agtaccggtt tttacagtaa
     1861 agattaaggc gtagcggctt tggcttgcag gcgtcgaatg gcgttttcaa gcgtcggagt
     1921 gtacgaggat tgatttttga gcaattgtgc agcgcgttca tataaagtga gcgcgttttc
     1981 tttttgattg agagattcca ttatcgcggc ccgggcataa aacaaacgac cgtaatacgt
     2041 cacaaaaaaa cgcgccatgc gatctttgtt gatcgcctca tcggtgaaaa tttcggtttg
     2101 gatattctta tgatctaaag atgtgggtcg aatcatatcc gtgcgtaaac gaatcgcggt
     2161 taataccgat tctttataat atttttcagc aatgcctttg gggggatcgt acgtgaggta
     2221 aacagaacga cccgattgca tccagttatc aatcaaagcg ttgatcatgc gttcgtatcg
     2281 ccgttgaata taattgccat caaatttttc gccggcttca aacggtttca cggcatctaa
     2341 aaaatcatca atttcttttt ttatcagttc ggtcgattca gggttgtgat cacggagcat
     2401 ctggatatac caacttcgcc ggagtaattc ctgatctatc ataaaaagat ccggtcttct
     2461 tttttcgacc tgctgataat agaaaaaagg aaaaaccaat ggatcccaat ttccgatcac
     2521 gagcgcattg gagtctgtga ctgaaaagat attttcggcg aagcattccg taaaacgata
     2581 ctgatgcata ttgtttcgtt tgtagttttc aggaagatgc cacaagcatg ctattgccag
     2641 caatcctgct gcaatataag tcactcgcga aggccattgt tcaaccaagg atatagttcc
     2701 taaggcaact aaagcggcta cgatcatata cgaaggaata tagaatacgg acaccagtgc
     2761 tttgttttca gcgctgacta agggattgcc ggtagtcacg tcaaagttgg taagataggt
     2821 cgtcaccggt gcggtgaaaa accaaaatac taaaacaaat agcaaaagtg aacgatgacg
     2881 cttccataaa aaaactaaag ccggaagtac caagacaaga atcggagaaa accactgttc
     2941 gccgagcagc gaaaaatatg tcttaagctg agagataaac ttaaccagcg tttgtgtttc
     3001 gttaagttgg tattgatggc gagcaataac gcgccaaaaa ttggtccatg tctcgggatt
     3061 accccaatcc atcatcggat ttcgtgccga cgctaatggt aaataaataa aacaagaaag
     3121 cccgagggtt ataaaacctg ttgcgataat tattcttttc agatttaata taaaattacg
     3181 atctgtaatg aatgccaaca tcacgagcgg tggcaaaagt acaagtgtgg tggtgtggtt
     3241 gctcagggag agacccaaaa caaaactcaa aagaaaaaaa taccgcgagc gttcattgtt
     3301 gccggtttcg ctcaaccata acgtggtcaa aagtagaatt atccctgtaa aaaaaatgtg
     3361 aaggccatac acttcggaga taaccgattg ctcccaagct tcagaggatg tcgcggtaag
     3421 aaacgcagtt aacacagaga atagaaatcc gagcgatgat ttttttaaaa tatctatata
     3481 tgtaagacgc aaccatagaa gaaatgtcca cgcaatcaaa gcggcaccgc tggcagaaaa
     3541 aaaagcagaa gccatattaa cgcggtaagc gatattgcca aaagggagcc acgtaaaaag
     3601 atgtgaaagt agcgtccaga gcggataacc gggctgatgg ggaataccta ggtgtgcggc
     3661 ggcggcgatc agttcgccgc tgtcttcaaa cgtcactgtg ggcgctaacg tattgagata
     3721 tagagcaaaa gtaatagtaa acgaagttat cgccgtcgtt gcaatgatga ccggagaaac
     3781 ggatactgtt gaagaatcgg aattgtttga tgagtttggc atgcgatcgg atttaatttt
     3841 tgaaaaatct aaataatcac aaacataacc acaagcatca aacaaaaaag acggccaagt
     3901 tgaccgtctt tcgaagatgt ttagatataa ataaactaca tgctgatttc gattcccata
     3961 cgtagttcgc ggcctggttc atacatcaga ccatttcgaa aatgtgcctt atactgaggg
     4021 cgtatccttt tttgatagtc tagaaaatac ggcgagtcta agaatccgtt gtcgtccggt
     4081 tttccggaag aggagaatac ctgtgttggg tttttattgt tatagaggtt ggtgcattcg
     4141 ataaataaag ttgctgtatg gtttttccat acaaaggact ttccggcttt gatatcaata
     4201 gtccaaaggc tgccggtttc actgctgttt tttttggata cgggatattg gttattatat
     4261 ccgacggctt gaataggagc catagtggtc ggggtataaa atgatccgtc tttaaaatgc
     4321 cattccatac ttgcaaaagt gcctttgagc aaaggttctg ctgcgctgtt gtcaaaatga
     4381 agtgtcgctt gctgcccgaa ttgccatgtt cttacatggc taaccggcac tgcaaaatta
     4441 gcagtgttaa aaccaagcca ggcgaagtgg taacttgaaa catttgaccc taattcaaac
     4501 tcggaagttt gtgcgccata attccaaata atgaaaaaat tctcgtgggg tatcagcgta
     4561 atatccgctg caaaaccaac aattcctgct ttcgggccgt tatagtacga aatatagccg
     4621 ttcggttcag ctactacttt ggtatagtca acgatgttct ttatttcttg tatataaaac
     4681 tgtgcgccca tggatgaagg agaagaaaaa tttacacgtg ctcccaaatc aaataattcg
     4741 gattccacag gttttaattt cgaattaccg atgatcgttt caaccgatgg cgccaaaacc
     4801 gttttttcaa gaaattccga tccgacataa cgccgttgta accctccggg tgtactgtac
     4861 cacccatagt ggctgtaagc cgataacatt tcgttaagtt gtatatttac tcctagccgt
     4921 ggtaaaaata gtatcgtggt atattcatga gagtaaaaat ctttttcatc taatacccct
     4981 ggggatacgt cattatacat gccggaagga tcatgaagat tacgaagctc tcgtgtacct
     5041 gaggttaggc gctggacatg cagaccgaat gttcccgaga aatttttcat tttaaaattt
     5101 cccataccgt acgcattgat cagtgttggc tttttgacgc catctaatcc cgagtttttc
     5161 cttttaagtt tgaaatcatc ggtaatgcta tatccgaaaa tatcgttact gccgaattga
     5221 agattttcgc tgtcatatat tgttgcagat cgatagatcc ctgaagtata agaaacgccg
     5281 gattcgataa atagcgtatt gctaaacata tactgcgatg aaacggaaac ggatgcggtg
     5341 gatgattcct tacgaataaa cataccataa ccggcgccga catcataaaa taaattgtca
     5401 ctcatatatg ttaaaaaacc ggtgcgtcct ctgtacgaat cttgtaaatg acgtaacgca
     5461 taagagtctg aagaggagag ttttctattg gttatcgttt ctttatttac atagtaacga
     5521 aggcccccgc tgaaggataa ttgctgatct accctccagt gaacggatga ttggatatcc
     5581 caagccgtat tttccgatcg gggaacaccg tacggcgata aaagataaaa catgttaaac
     5641 caattccgtt tcatttgcga aaatgtgcct gaaaactcca ggtttaaatt ctgatccaaa
     5701 ggcgccgtaa agcgagcgta agcattgagg cggcgcaacg cattggaacc gttaggccga
     5761 gcgccgtttt taatatttcc ccaaagcacg gtatcaatcg gtatagatgc tgtggcatta
     5821 ttattatcgt gatcgtacag ccggctgcct ttggctatcg tataacgagg tactccgact
     5881 acgctgggat cggcatcatc aaatgattcg tactcagccg ctgagtacat gtataacttc
     5941 tgaggaataa tcggaccgga cacttcgcca tcaaaaacat gttttccata cgaaggtaag
     6001 cgcaataatt tggcgggcat atcgctaaat atagaaccag cggcaaacca agaggtaccg
     6061 ccttgtttgg tctgaatgtc tgccgtacgc acgtgattgc cagtgcgcgt atcggaaccc
     6121 ccctcttgca ataaaacgga agcaatggcg cggtgaggaa gctgtatatg atgacggctg
     6181 tcgaaaacat cggtagttgg gaatccgttg acccgataac cgagcgcatc gctaaaatcg
     6241 gaacgaatat ggataacacc ggactgagat tccggatcaa ttacaggaag tataggtttg
     6301 gttatgaacg agcgttcaat cgcatcgtga gaaaagttgg tataaatcgc acccggcata
     6361 ttgagaataa atccctcggc atgacgtccg ttttgcatag tctgagtttg ggctgagttt
     6421 gggctgtttt taggcgcact gtatgtgaaa agtacttttg taccatcgtt tcaggattag
     6481 attgtccccg gtagatttca agttttccgt tttcgagagt taaatctatc tctgtcgggt
     6541 agtcacctga aatatataca ttcacgggtt cagccgatgt atccgtttcg tcggctaagt
     6601 gtaacgcgta tacaccggac tgcaaaagat aaaaacgtac cgttccttct tcgttggtga
     6661 cggcgctata cggtgtttgt gctaaagcaa tgcgcgagcc ggctaccgga gatccgtctt
     6721 ttgtggtgat acgtacgatc aattccccaa aatccccggc gaaagcaacc gatgaaaaaa
     6781 cggcactgaa aaatgcagcg cgaagaaaat gtaataacga catggatttt ccctcagata
     6841 aagtcaaaaa cagaatgaga tttgataaaa gcatggtaag gttaaattag gcatgattta
     6901 aaaacaagag taaagtcgga tcaatttgaa aaaactaacg acatgtgtta tcaaataact
     6961 acatcgcaaa tacaatacgt gcgtaatatc aatccaaccc aaaaggttag tcaaaaggtt
     7021 attatagagg ggagtctggg agatatgtat aaactctttt caggattgga gatgttgcga
     7081 aatagactga aggtaagagt taaaaaaaga aattattttt ttataaatat tctttgacaa
     7141 tcacaggcta ctattctatc ttagccgcca ttcggaaatt atggattaat aactgtttaa
     7201 ttccacattt tgagaaagtt cgccacggga tgaaagaaat cctgaaatct tatctgacac
     7261 tgaccaaacc gacgattatg ttgctggttg tttttacggg cgcgacggca ttaatcgttg
     7321 aaggcagttt cttacatgag ccggtgaaat ttattctcgt actggtgggt ttatatctta
     7381 ccggcggttg tgccaacgga ttgaatcagt ttttcgaacg cgatattgat gccaaaatgg
     7441 agcgcaccaa aagccgtcgt cctttgccgg gcggaatcct gagtccgatg cacgcgctga
     7501 ttttttgctc gattatcggt gtctccggcg tgattctttt cgccgtgact ttcaactggc
     7561 ttacggccat cttatcttta gcaactattt tattttacag tttcttctat accctttatc
     7621 tcaagcccaa tacgtcacag aatatcgtta tcggcggtgc ggcaggggcg atggccccgg
     7681 tcggtgcctg ggccgctgcg accaatacaa tggcgccggc gccgtggatc atgtttctga
     7741 tcgtgttttt ttggacgcca ccgcattttt gggcgttggc gctgttttgt aaaaacgatt
     7801 atatcaaagc caaattaccg atgatgcctg tcgtcaaagg tgatgacagt acactccgtc
     7861 aaatgtttta ttattctctc gtgctggtag ttaccacgct ggctcttatt ttcttcaaat
     7921 ccggttggat ttatgccgtt acggcgatta tcctcggttt cttcttcgta aaaaaagcgt
     7981 acgacgctat gaaatataaa actgaaaaaa tcattcgcgg tctttttggt tactccattg
     8041 tgtatctttt tgcgcttttt ttggccatga tagtggacag tctcgtcgct taagtgacga
     8101 acaatctagg taattctttg catttgtaaa gaatttgtaa aattggcttg caagtttaga
     8161 catgtccgtc taaattgctg tcccgtgaac cttaattgat gtcccccgag tttgactgtt
     8221 agaagttttt aagattatcc accgtttaat tattcattaa tgaggagacc tcagtggctc
     8281 agatattccc aaaaagggca aacaagactc cgattttagc tgccgccgct ttgggatttt
     8341 tgggtatggc cgtgaccggt tttttctggt actacggttc gcctaaattt accgatgtag
     8401 gctacaggcc tgtacaacct gttccgtaca gtcacaaatt gcatgcaggt gatctcggca
     8461 tggactgccg gtattgccac gtaggtgttg aaaccgccgc cgccgccatg gtgccgccga
     8521 cggctacgtg tatgaactgt cacaaacttg ttctgccgca aagtgaaaaa cttttgcctg
     8581 tgcgagagag ttttgcaaac aatacgccga ttcaatgggt caaagttcac aagatccctg
     8641 attatgcgta cttcaatcac agcattcacg tcaatcgcgg cgtgggctgc gaatcctgtc
     8701 acggcaatat cgcgcaggaa gaagtcgtcc agcagcgtca acccctcagt atgagctggt
     8761 gtctggactg ccatcgtaat cccgatcagc atctccgcga tctccaaagc ggagtgaaag
     8821 taacggatat gaattggacc ccgccggccg atcaagccaa gtttgccgca cgtatcaaac
     8881 aagagaaaaa aattgcaccc ccagaagatt gttcaggatg tcaccgatga gccaaaagac
     8941 tacacttaca caaaacggta aagaatactg gcgcagtctg gatcagctcg ccgacacgcc
     9001 cgaatttaaa gaattcgtgc agcgtgaatt tccggagcaa gcttcggaaa tgagtaatcc
     9061 ggttacgcgc cgtactttct taagcctcat gggcgcttcg atcgccctcg ccggtctcgc
     9121 gggttgccgc cgtccggtcg aaaaaattat tccttatgtt attaaacccg aagagatcat
     9181 tcccggcgta gctgaatatt tcgccacggt catgcctttc ggtaccggcg cctatggtgt
     9241 cgtggccaaa agccatgaag gccgtccgac caaggtcgaa ggtaatgaaa aacatccttc
     9301 ttccctcggc gcgaccaatg cctatgctac ggcttccgtt ctcaatctct atgatcccga
     9361 tcgttcagcg tatgtgctga ataagggcaa tcagaaattg tggaatgatt tcgtagccgc
     9421 atggcgtgaa ctttttgcga agtacacggc caataagggc gaaggtctcg cgatcgtatc
     9481 cgaatcgttt gcctcgccga caatggcgcg cttgcaggcc gaatttacca aggcgtttcc
     9541 taaagcgacc tggacttctt acgaagccgt cagtgatgaa aatatctatg ccggtatcga
     9601 aggtgttaca ggtaacaaac ttcagccggt gtacaattac ggtaaggccg gtgtgattct
     9661 ttcgctggac gccgacttcc tgcatatgga atcggataat atcaccgcat cacgcggatt
     9721 tgccgatggc cgtcgcatga aagacgaaaa cgatacgatg aatcgtttgt atgtcgtcga
     9781 aagcatgttc accctgacgg gcggcatggc ggatcatcgc ctgcgtcttc aaagtcgtca
     9841 gatcggcgct ttcaccgttg ccttagctaa agctttgggc gtagatacga tgggtatcaa
     9901 tgccgacact tccgtattgg atgaaaaagc caatgcgtgg attgctgcgg tcgctaaaga
     9961 tctgacaaat aacaaatcga agtcgcttat tgtcgcaggc cgtcgtcagc cggctgccgt
    10021 gcatgccttg gttctcgcga tcaacagtgc actgggcaat gtcggaacga ccgtgcagta
    10081 tcatgctgca aaagatatga tggtgtcaga ccgttccggt ttagccgaac tgaccaaatc
    10141 cttgagcgat ggcaaagtat ccaccctcgt catgttaggc ggtaatccgg tgtacaatgc
    10201 acctgccgat ctcaaatttg ccgaggcgat gaaaaaagca gaacatacca ttcacttcaa
    10261 cggctacgtg gatgaaaccg gttccaatgg tgtcgaatgg catatccccg caagccactt
    10321 cctcgaagca tggggtgatg tgcgttctgc taacggtacg gtcagcgcga ttcagccttt
    10381 gattgatccg ttgtatgcaa cacattctga cattgaagtg ttgtacttac ttaccacggg
    10441 taaagaagcg cgcgggtatg atatcgtacg cgaaacatgg aaaacactct ttgccaaagg
    10501 cttggatttt gagaaagcat ggcgtttggc attgcatgat ggcgttgtcg gtgatacggc
    10561 tacggcccct gtaaccgtca aaatggacgc taaagccgcg agtgcatttg ttgcaaaaaa
    10621 tgcgttgccg tcaggatctg cggatgccaa taacttagaa atcgttttct atgcatcgtc
    10681 cgctactttc gacggtcgtt ttgctaacaa cggttggctg caagaattgc cggatccggt
    10741 gacgaaaatc acgtgggata atgcggccat catgagccac aaaacagcag aagcgctcaa
    10801 agtcaaaaat attctcggcg aaggtaaaaa tcaccagccg atgattcgat tgaaatatca
    10861 agggcagacg ttggaacttc ccgtatgggt tcaacccggt catgcggatt attctatcgc
    10921 tgttgcttta ggatacggac gccaatcggc cggccgcgtg ggaaatggcg taggcgctga
    10981 tacgtatgct ctgcgcacat ccgccgccat ggattttgat ctcggcttga cagcggagaa
    11041 actgtccaaa acgtatttga tcgcaaccgt gcaggatcac ggtggatttg atacggacgc
    11101 gcttgctgat aatgaaattc aacgtcgtat cccgaccatt atccgggaag cgacgatcga
    11161 agagtacaaa aaagatccga attttatcac cagcggtcgt acgcccggtg aaaaagaatt
    11221 gtacggaaaa gacggcaagc cgatttcgat catgaatatg cacgaatacg gcccggaacg
    11281 atttgaaaaa ggcgagtgga ataaaaaata tcaatggggt atgtccattg acctcaatgc
    11341 ttgcacaggt tgtaatgcgt gtatgatcgc ttgccaaagc gaaaacaata ttcccgtggt
    11401 cggtaaggaa gaagtggcca agggccgcga aatgcactgg attcgcttgg atcgttacta
    11461 tatcggtaaa gatgtgaacg aaccggaaat gcagttccag ccgatcgctt gccagcactg
    11521 cgaaaccgcg ccgtgcgaac aggtatgccc tgtcgctgcg acagtacacg atagcgaagg
    11581 tctcaacgta atggtgtaca accgttgtgt cggaacccga tattgcgcca ataactgtcc
    11641 ctacaaagta cgtcgtttca atttcctcga atttcatgcc ggtacgtccg gatttatgga
    11701 atcgaaaccg atcgaatccc tcaaattggg caaaaacccc gatgtgacgg tgcgtatgcg
    11761 cggtgtgatg gaaaaatgta cgtattgcgt gcagcgtatc agcggtgccc gtatcgcggc
    11821 gaagaaagaa gaccgcgata tccgcgacgg cgaagtcgtc acggcatgcc agcaaagctg
    11881 cccgacggga gccatcgttt ttggcaatat caacgatccc gaaagcaagg tgtccaagat
    11941 gaaagccatg aaccgcaaat acgaagtgtt ggccgaactc aatgttcgtc cgcgcacgag
    12001 cttcctggcg aagctgaaaa atccgaatcc ggaattaaca aaaaacgctt aactataaaa
    12061 cgagtaacgc tgtgagtgta gcaattcaag aacatcatac ggccgacgaa ccgcttatca
    12121 cgagtaacgc cacgttctcg ggtataacga atcaagtcag cgccgtcgtt gaaaacaaac
    12181 cgccgcgtcc atggtatgta ttttttgcca tggccagctc cggctttctg ctcctgctga
    12241 tcgcgatcac gtacctgctt tttgaaggta cgggtatctg gggtatcacc aatcctgtcg
    12301 gttggggtct tgcgatcgta aacttcgtgt tttgggtcgg tatcggtcac gccggtacgc
    12361 tgatctctgc gattcttttc ctttttcgtc aaaaatggcg cacgtccatc aaccgctttg
    12421 cggaggcgat gacgattttt gccgttatct gcgcgctctt attcccgggt attcacgtcg
    12481 ggcgtatgtg ggcgacgtat tggttcgcac cggttcctaa tcagatgggg atgtggccta
    12541 acttccgcag cccgctgttg tgggactttt tcgccgtcgg tacgtatttt accgtgtccc
    12601 tgattttttg gtatgtcggc ttgataccgg atttggccag cttgcgtgat cgcgccaaaa
    12661 cgcgcgttcg tcaggttgtg ctcggtttct tgtcgctcgg atggcgcggc ggtaataaac
    12721 agtggaaaca ttatgaaatg gcgtatttga ttctcgccgg tatttccaca ccgctggtgc
    12781 tctctgtgca cagtatcgtt tctaccgact tcgcgacgtc gatcattccc ggatggcata
    12841 cgacgatatt tcctccgtac ttcgttgccg gcgctatttt ctccggcttc gccatggtaa
    12901 tgacacttgc gatcgtagcg cgtaaagcat ataagctcga aaacatcatc acgttatacc
    12961 acttggagaa catgaataag atcatgctcc tcacgggtat gatggtcggc tatgcttaca
    13021 gttgcgaatt tttcatcgca tggtacagcg gtaatatgta tgaacgattt gcgtttatca
    13081 atcgcgcttt cgggccgtat tggtggtcgt atttcagtat gattttctgt aacgtcgtcg
    13141 taccgcagat tttttggtct aagaaattac gtcgcaatat cacggtaatg tttatagctt
    13201 cgatcttcat caatatcggt atgtggtttg agcgttttgt gatcattgtg actttgtcac
    13261 gtgattatct gccttcaagc tgggattatt attcgccgac gatgtgggat tggtttacat
    13321 tcctcggatc cttcgggtta ttctttacgc tcttcctgct tttcttgcgc tttgtgccga
    13381 tgattgccat ggccgaagtc aagggcgtat tgccgcaggc ggatccgcat catcacaacg
    13441 gaaaacatta atcgggatac gaatatgagt gcatcaaaag aaaaacaaac agatgaagtc
    13501 gcaatcgttt tggccgaatt tgacggcccg gcgacattga tcgaagccgc caaaaaaatg
    13561 cgggacgccg gatataaagc gtttgactgc cattcaccgt tcccggtgca cggcatggat
    13621 caggcgatgg gtttagcgcg ttcccctttg gctaaaatat gtttcgtcat tgcgatgagc
    13681 gctgtgcttg gtatggcgta ctttacatat tggctcagtt ttgattacaa attggttatt
    13741 tccggcaaac cgttattcag ctatgttgcg tacgtcccgc cggtatatgc ggtcggcatt
    13801 ttgacatgcg ctttgacggc ggtggtcggc atgatggtgc tcaacggtct tccgcaaccg
    13861 taccatccgc tgtttaattc ggataatttt gcccgcgtta cgcaagatgg attctttgta
    13921 agcgtggaat ccaccgatcc gcagtatgac gaggccaaag tcaaatccct tctggcgtcg
    13981 ctcggcggca ccaatgtgga agttgtcaaa ggataattca aacatcttaa ctatagtgtc
    14041 tatgaaatct tcaaaaattt ggtccacgac ggtgattgta gcggtgatgg cgctgagtgc
    14101 ttgccagggt cgtccctcgg aagaaccgcc tattcacttg aaccccaaca tggacagtca
    14161 gcctaaatac aaggcgcagt ctaaaggtga tttttttgcc gacggcatgt ctatgcgtac
    14221 gccgccggaa ggaacggtcg ctcgcgggca actgcgtgaa gacgcggcgt actacatggg
    14281 tcgcgacgaa aaaggaaatc ttctcaaaga aaacccggtg ccggtgacca tggccttgct
    14341 tcatcgcggt caggaacgat ttaatattta ttgtacaccg tgccacggcc agaccggatt
    14401 gggcaacggg atcgtcgtac agcggggtat gttgccgccg cctaattttc acgatgaacg
    14461 gttgctcaaa gtcgaagacg gtcatttttt cgatgtgatg accaacggta tccgtaacat
    14521 gtcttcctac aaaagccaag taccggtagc ggatcgctgg gctatcacgg cctatatccg
    14581 cgccctccag cgtacgcata cggcgtctct ggaagatgtt ccacaagaaa aacgtaagga
    14641 cttgaagtaa acttatgaaa ctgaatactg aaacctttcg tctggaagac gccggttcat
    14701 tcggtaaaaa agcgcttatg gccggtattg tcggtctttt gatcagcgtt gccggatatt
    14761 ttgtgaatgc cgatcaattc tggcgttctt acctcacggc ttttactttc tggacatcca
    14821 tcggtcttgg cggtatgttc ttcgtattgg tcaagcattt ggccggtgcg gagtggagtg
    14881 tggttttaag acgtctggcc gaaaacttca tgatcacttt gccgatcatg tttgtatttt
    14941 ttcttcccat tttattcggc agccatcatt tgtatgaatg gacacatacc gaagtggtcg
    15001 ctaaagaccc gattctgagc gccaaatccg gttatttgaa tatgacgttt tttaccatcc
    15061 gtacggtgct gtattttgca atatggacga tttttggccg tatgctctac aagatgtcaa
    15121 tcggtcagga tggcggcgat caatcaaaaa ataaccgcat gcgccaactc agtgcgcccg
    15181 gtatgatttt gtttgccttc tcgatcacgt atgcatcatt tgactggatc atgtcattgc
    15241 aaccgcattg gttttcaacg atttttggcg tgtggtattt tgccggcagc ttgctcaatt
    15301 ttcttgccat gctggtattg atcgccattt atctgcgcaa acgcggtgtg cttgataatg
    15361 tgatttcgat agatcacttc cacgatctcg gaaaacttca attcgctttt actattttct
    15421 gggcttatat cgcattttct cagtttttcc tgatttggta tgccaatgta cctgaagaaa
    15481 cgattttctt cctcaagcgc tgggaaggct cctggatgta tgtcagtatc ggattacttg
    15541 tttgccactt gatgattccg tttattttac tcattccgcg tgcgaataaa cgcaatctta
    15601 cgattatgaa gtttgttgcg atttggtttt tatttatgca ctgggtagat ctgtattgga
    15661 atatttttcc gacgtggcat gatgcccatc acggtgtagt aatgtccggt ttatgggtgg
    15721 acgcggcgac aattttgggt atcggcggta tattcctttg gtacttttgg atgctgctgg
    15781 gcaaacaagc attagccgct cacaaagatc cgagtttcga aaaatctatg catcacgtta
    15841 atctttaatc tgaggatacg ggcgatgagt gaaaatacac aagaacaaat ccatcccgaa
    15901 gacggctatg agaaatccga tgtcaatgca cgtgctattc tggtatctac attgattggt
    15961 atcattttga tcgtcgcgtc gctggtgctg gttgatgaat attttattca aggacgtgaa
    16021 cagttttata ctgaagcggt tctcaaaccg gaatcggccc ctctgcgtga cttgcgtacc
    16081 aaagaagcgg aatttctcaa cggatatgca gtgattgatg ccaaaaaagg cgtttatcaa
    16141 ataccgataa ccgtcgcgat ggaacgtttg gccgcggaag ctttcgagaa aacgcagacc
    16201 aaaaaagacg gtaaaatttc caaaaaataa agtctgatct gaaagaattt tcatgcgatt
    16261 acgatcgcta ctttgtataa ctgttctttt acaatatgta tgttctacgc tggacgcgca
    16321 ggtgattctg gagaagaatc aggatcccga gttgcaaaaa atagatgtga tcgaacatct
    16381 gggggatacc gtcccgatga atttgcaatt taaagatgag cacggagcga tagtacaatt
    16441 gtcggactac ttcgaaaaag gtaaaccgat cgttcttacg atggcttatt atcgctgtcc
    16501 gatgttatgt acgatggtgc tcaacgcgaa agccgaggcc gtcaaacaat tagcatggtt
    16561 acccgggcgt gaatttcaaa tgctgactgt tagcatagat cccaatgaaa caccggaact
    16621 ggcgtcgaat aaaaaagaca cctacctgaa gtttcttgat aagtccggta ttcaggaggg
    16681 ctggcatttt ttggtcggcg attcgtcgca gtccaaagcg ttggcggatg cattggggtt
    16741 caaatatttt tacgatgaaa atcggaaaga atatgcgcat ccggcggtag tctttgtgat
    16801 caccgaaaaa ggcatcgttt cccgttattt gtacgggata cgatttgctg aacgcgatat
    16861 gcgattggct ctgatggagg cgtccgaagg aaaaatagga agtacgattg accgcatcat
    16921 tatgtactgc ttccattatg atccgaatgc caaaggttat gtagtcatgg ctacaaatgt
    16981 gatgaagtta ggcggattgg tcacgctggt tgtgctgggt gtttttttaa cggtgatgtg
    17041 gcgtcgcgat aaaacacgaa gtattcaata aattttaata taaattctat aattaaagga
    17101 aaacgatcgt atgaatgatt tttggatgcc acccgcgcat tcgacggttg cgcatgagat
    17161 tgacagtctc ttctatttca tttattactg ttctgtcttt ttctttgtgg ctgtcgtcgg
    17221 cgcgatgact tactttgcgt ttaaatacaa acgtaaagcg catgaaatga ccgaacctgc
    17281 agcgcataat aatacgttgg aaatcacgtg gacggtgatt ccgacgattt tgtgtctcgt
    17341 tgttttcgtg ttgggttttc gcggctacat gaagatgaat gtcgttccca aagatgcgct
    17401 tgaaatcaag gcgacggggc aaaaatggtt ttggtctttt gattaccctt ccggcgctac
    17461 gacagtaaac gatattgtca tcccggtcgg aaaaccggtc aaacttctga tgtcctccaa
    17521 agatgtaatc catagttttt acgttgcaga atttcgtacc aagcgcgacg tattgcccaa
    17581 ccgctactcc atcacatggt ttgaagcgac acaagccgat acgtttttga ttcagtgtac
    17641 ggaatattgc ggtacaagcc attcggaaat gatcggaaaa gtgattgcgg taagtgaaac
    17701 agattacaat aaatggctcg aagccaatag caatccggga gaaggtttga cccctgaagc
    17761 ctacggtgaa aaactatata catccaaagc gtgtattaca tgccatagct tggacggtaa
    17821 acccggttca ggtcccagtt ggaaagcgat ctttggtaaa gctgaaaaaa tgaccgatgg
    17881 ttctactgta acgattgatg aaaattattt gcgtgaatcc atactaaacc cgcaagccaa
    17941 aatcgtggcc ggttatcaac cggtgatgcc gacgtatcaa ggcattctga aagaccgtga
    18001 gattgacgct atcatcgctt atatcaaaac tcttaaatag aaggtaatta atatgtctgc
    18061 gacagtgacg gaaaaaaatg tgagttcggc gtccacaggg atgcctgagc atcacttttt
    18121 aaacagcccg caagggttga agtcttggtt gttgacgctg gatcacaagc gcatcggagt
    18181 gatgtacctt tttggtatca tgacgatgtt ctttatcggc ggtgtattcg cattgcttgt
    18241 acgcctcgaa ctgatgagtc cgggcgaaga catcatgggc aaagacgctt acaataaaat
    18301 gtttacactg cacggcgccg tgatggtgtt tctttttatc gtgccgtcaa ttcccgcaat
    18361 tatcggtaat atcattctgc cgttgcagat tggcgcaaaa gacgtggcgt tcccgcgtct
    18421 caatttagca agttggtacg tatatcttgc cggttcgatg tttgctattt attcgatgat
    18481 taccggcggc gtggatacgg gctggacatt ttatacaccg tatagcaata ccagtaatac
    18541 gtctgttatt tctatgacga ccgccgtatt gatccttggt ttctcgtcca tttttaccgg
    18601 ccttaatttt atcgtcacgg tacacaaact gcgtgcaccg ggcatgacat ggttcaaaat
    18661 gcctttgatg gtatggggta tttatgcgac ggcattgatt caggtactgg ctacgccggt
    18721 gcttgcgatc acggttgttc tcctcatgct ggaaaaaatg tggcatatcg gtatttttga
    18781 tccggctatg gggggtgatc cggtgttatt tcagcacttc ttctggttct attcgcatcc
    18841 ggccgtgtac attatgatct tgccgggtat ggcgatcatt tcggaactgg tggctgcgtt
    18901 ctctcataaa aatatgttcg gttatcgttt cgtagcgttt tcgtcattgg cgatagcgct
    18961 cttgtctttc ctcgtatggg gacaccacat gtttgtcagc gggcagtcgg aattgtcagc
    19021 cgtgattttc tcattcctta catttttcgt cggtgtgcca tcaggtatta aaatattcaa
    19081 ttggcttacg acgatgtata aaggttcgat cagtttttct gcgccgatgc tgtggacatt
    19141 ttcattttta ttcttattta cgatcggtgg tcttaccggt attttcttgg gaacgttgtc
    19201 agtggatata cacttacatg atacgtactt cgtcgtggca cattttcact acgtgatgat
    19261 gggcggtacg gtgatggcgc tgatgggcgg cctgcactac tggtggccca aaatctgggg
    19321 ccgcatgtac aatgaatttt gggcacgcat ttccgtattg ttggttttca tcggcttcaa
    19381 tatgaccttc tttactcagt ttgtcatggg ttcccgaggc atgccgcgtc gttactggac
    19441 ataccaagat caattcactt cgttgcacgg tttttcgtcc atcggtgcgt ttgtgatcgg
    19501 agcgggattt ttgatcatgg cgttttattt gattcattcc atattcaaag gcaaaccggc
    19561 gggtaataac ccttggggcg ctctgacatt ggaatggacc acgagttcac cgccgccgca
    19621 tcataatttt gatcagacgc cgatcgctgt caatggcccg tacgactatg acaaggtcaa
    19681 ggcggaaatc acatatgaac cgaacgttcc caaagcgaaa gtataatttt tttaacgata
    19741 acaggtaact atgtcaagtc aacattcagc cgctcatccg gcgcatctgg cgcatcactt
    19801 tagtgatgtc gagcagcaaa aagaatccgc caaactcggc atgtggttct tcctgctgac
    19861 ggaagttctg accttcggag gcctattttg tgcatacgcg atttatcgcg cgtggtatcc
    19921 ggaaatgttt tctattgctc atcaacaact caatgtcacg atgggtacga tcaatacgat
    19981 cgttctgatc acgagttccc tcacgatggc gttggccatt cgcaatatgc aactcaatga
    20041 caaaaaaggt acgctcatca tgcttctgct gacgttcgtg tttgcaggaa catttatggt
    20101 gatcaagtac tttgagtaca cgcataaaat tcatctcggt caattacccg gaaaatttta
    20161 tacgtttgac ggcatcaatg catccaatcc gcacattttc ttcagcattt attttgcgat
    20221 gaccgggttg cacggtattc acgtaatgat cggtatgatt ttgattgcct ggctaatgta
    20281 taaaaccagt aaaggtgcgt tttcatcgga atattatacg ccgatcgaaa tgacgggcct
    20341 tttttggcac cttgtggatt tgatttggat tttccttttc ccactctttt atttgatagg
    20401 atgaggccat gagcaacgaa caacataaag aacatattat accgttgagt acgtatcttg
    20461 gcgtttttag cgcactgata gctctcacgg cgctgacggt atggatcgcc caatttcact
    20521 tcggcgaatg gaacctgatc gtggcgatgt ccgtcgcagt agtgaaagca cttttggtag
    20581 cttttatttt catgcatctt ttgtatgata ataaattgta cgccacggtg tttgccggct
    20641 cgattgtatt tttagccgta tttattgtat tcaccatgta tgatacattg cgtcgtgatg
    20701 atatctacga acaagtaggt aaacccatca agtccaaagc gatcatctat gataatatgc
    20761 ccgtcaagaa agattctacg gcgcatagcg gacattaata ggatccgtga aagcgatttg
    20821 atttgaaccg atttcggcaa tttgccttat tttcaaccat cgccacgtac gtacttattt
    20881 tcgttggcgg tttggtacga gtttcagggg ccggtctggg atgtccggac tggcctcaat
    20941 gttttggtca ttggtttcca ccgaccagtg tagatcagat ccctccccat attgacccca
    21001 gtctttttaa tatgacattg gcctggatcg aatacggcaa tcgtattgtc ggtgttacga
    21061 taggctttct tattgccggt acggcttggc ttgctataaa acattaccgg catgacaaac
    21121 gtattttgac agcgtcagtt gcagcagcga ttttggtggc tttccaagga tggcaaggga
    21181 gtcgtgtcgt caagtcccaa ctcaacccgc atatcatctc catccatttt gctttggcgc
    21241 ttttgatcat cagtctgatg gtttacgtca ctttccgcgc atataaacct gaacaagaaa
    21301 taaagaacat cagcgatgcc gtatatcgtt ctgttttcgg ttggataaag attatgtggg
    21361 ttgtcaatct cctgcaaatt ttacttggaa cggaagtacg gggtgcattg gaagtattgg
    21421 ctaatgcatt tccgttgatg agcgaagccg aactaatgcg ccaatcgaat tatgtcagct
    21481 atattcacgg tgtcgtcggt gtgatcatcg taggagcgac ctggcttttt cacgaacaaa
    21541 tacagcgtgt gcgcacacaa atcccggcct atatcgttac gatttcaaat atcggccttt
    21601 tacttatgat catccaattg ttcatcggtg tgctcctggt aacgatggat cgtccgccgc
    21661 ttttgcaaat cgtgcatctt tggatatcca gttttcagat cgggttgttg gtgatctttt
    21721 atgccgcatg gcgccgcgat aatcaatctt ctgagaggaa aacggtatga atacgaatct
    21781 gattatgcgt ttaagtatcg gtttttttgt gctcatgctt atttcggcga tcgttattta
    21841 taaagccaaa gaatcgcaga gcgatttgcc ggtgtatgcc gaagtaacgc cgtttgccat
    21901 gaccgaacga agcggtgagg cttttacaaa cgaaaagttc aaggggcaca tcaccgttgt
    21961 caattttttc ttcgccacgt gtgcggggcc gtgcccggcg atgaatgccc gtgtagccga
    22021 gctttatcgc gcttttgcag ggaataaccc cgtacggttc gtgtcggtca cgatagatcc
    22081 ggatcgggat tcgctggtcg cgcttcgtaa ttatgcgatg tcgttcaacg taaacgataa
    22141 tcgctggtct tttttgcgcg gcgatctgaa aagtgttgag cacctgtcgg aaaatatttt
    22201 tatggtggga agcggagcac cgacactcca tagtacgaaa ctgattttag tggatcactt
    22261 gggccgaata agaggttact atagcagcga agaacctgca agtctgacaa tactcaaaac
    22321 acatatccgt gaacttgtaa gagagatgcc atcatgaacg ccgctgattt gccatttatc
    22381 aatgccatac tcaattccgt gagcaccgtg tttcttacgt tgggatacat agctatccga
    22441 aaaaaagatc gtgttcagca taaacgctat atggtcacgg cgcttatttc gtcagcgctt
    22501 tttctcacgt gctatctgat ttatcattat caggtcggtt cggttcctta tccgtattac
    22561 gattggacaa ggccggtata tttcgtcatt ttagtgccac acattatact ggccgcaatt
    22621 atgtcgcctt ttattatcag cgccgttcat tatgcatgga ttggggagtt tgcgaaacac
    22681 aaaagactaa tgcggtgggt ctggccggtg tggatgttcg tatcggtgag cggagttgtt
    22741 atctatctga tgttatatcg cttctaaatt aatcttcatt ttgccgacaa tgcgggcaaa
    22801 ttcgctttcg acgaaaggtt tgtacgacga gaacttagca gaaaacggat gtttgatttt
    22861 attttttgta gcatccatca aatcggctat caattctttt tgtgcattga tgaggtcgtc
    22921 gtaataaata ggctcgcccg catccttacc ttcgttcttc attttttcag cttgcgtatc
    22981 ccttaaaaaa tcaaatccct tttctgccgc aagcatggca ataagggggt gagcttccgg
    23041 cggggccgta ttaagagggt gaaacaaggt atctttgtaa cgatccataa tttcatccac
    23101 ggaatctctg tttcggctgc attttttaag caggatattt tcaaaactaa gggcatagca
    23161 tgcagaagct tgaggtatat ttcctgtttg aaaatagagt gtgcccaagt ggcttaggga
    23221 aacagccagc ttttcgattt caacattata cagcgtttca tcgcccaggt ccgggacgcg
    23281 cgctgttttt acgacgtcaa acacgcgagc aaaattgtct ttttcgcgtt caataaaact
    23341 aaaattgact tcgccgcgcg aataacgttc ggctttgagc aaagcatcgc accaataatg
    23401 gccgatcgta atggccgata tataatctac aaattccttg ttttcagtga ctgtttgcag
    23461 aacgccatcc atgcgggaca aacattcttc gtagcgctgg ccgcgaagca gaaaaaatac
    23521 caactggggc atgacataag caaccaactc aggattggaa agtttggaag ccaaatcttc
    23581 aatgatttcg atttcctcat ccaacacttt ttcctgaaga cgattgacga cacgttctgt
    23641 cgaaacgcgc agtgcctcct gaagttgtag atgcagttcg ttgcgcaggc gtggataatc
    23701 cggtagtaac cggaacagct caatagcgcg ctcgcacaat aaactgccct gtgcaaaatc
    23761 agccgccagc gaggtgtgca tcgcagtctg gcacaaggaa taaatcaaaa tactcatcgc
    23821 ccgatccgaa cgcgcgtgat gcgatttcaa gtcggattca aatttttgaa tggttttgcg
    23881 gatgtctgaa gtggaacgat gatgaagccg acggctcaaa aggcccacta aaagtaacac
    23941 caggatacgc tgatcttcga tcaaagaaat gacattttgt cttaaaaatt gggcatagtt
    24001 tccgcgaact gtttcatcag tttctttgcg caccggtttt tcattcgttt taggccgcat
    24061 ctggattatc ttattccagt actcgcgcaa tgggccttgc ttgtgtgtgc cggtgacgga
    24121 ggtaggatta tatacggttt ctaaggtacg gcgatttttt tcaaacgtac gcaaaagcgg
    24181 agcggaaaca cgaacctcat ggttttgatg aaacaaatgc aaaagatcgg cataagtttc
    24241 gcgtatgcag tgccatgatg atttattgga ttctaccgcc agttgcgcga gaatatttac
    24301 aagactccga atttcgccgt tagagcggcg ctcttcctga agcggtgcag tagacataga
    24361 ctatttagat acgtttattt aggtgtgaga tacccgatta ctaatcaact aatttatcat
    24421 aaaatcgggc gttcgcaagg caaaaatcat aacagggtat ttaatttatc agctaaaata
    24481 aaatccaatt ccgtaattcc gccgacatca tgcgtataga gtgtaatatg aacgcggtta
    24541 tatacgttgg accagtccgg atggtgattt aatttttcag cgataagagc cgctgccgtc
    24601 ataaaacgaa atgcttcgac aaaatcggag aaaataaatt ctctatacaa cttgccgtcg
    24661 cggagcttcc aatccggaag tgttttcagg cgattttcaa tttcataggt ttcaagtttt
    24721 tttcgactca tggttacagc tttttgaatc ggcgagttac ttcatcccaa tgaatgaggt
    24781 tccaccatgc ttgtacgtag tcggcgcgtt tgttttggta cttcagataa taagcgtgct
    24841 cccatacgtc caaagccaag agcggcgtgc ctttaaaatc ggaaacatcc atcaatggat
    24901 tatcctgatt aggcgttgag ccgatttcga gttttccgtc gcgtacaacg agccatgccc
    24961 acccggagcc aaatcggctc atcgctgtgg cgttaaattt agttttaaat tcatcaaagc
    25021 ttccgaaggc ggtattaatc gcagctgcca gttcgccgct cggtgcaccg ccgctccccg
    25081 gtttcatgat ttgccaaaac atggagtgat tccaatgccc gccaccgttg tttcgtatgg
    25141 ctgtcgaata ttttgaaata tgacgcatca aagtatctaa atccggttta tctattttag
    25201 cctgaatgac ggcatcgttg agtttatcta cataggcttt atgatggcgg gaatgatgga
    25261 tctccatggt ttgggcatca atatgcggtt cgagcgcttt gtaatcatag ttcagcgtcg
    25321 gcaatgtaaa cggtgcatcc gtcatgctta cggcagaaat cgcggcttcg gcggaacgga
    25381 taaaggggct taccgccgcc gccgttgcaa ctaaaccggt tgtttccaaa aatttgcggc
    25441 gtgttgtttt tttttcaggc ataaacggct ccttatgtgt tggagttttt tggttcaaaa
    25501 atgcgccgtg caaggccaag aatgacaaag cgcggaaaga ggcgctgtag gtgtgtcata
    25561 tatttattgc ccagcccttc gatggcgatt tttttataat tcactacggc atccaaagcg
    25621 acttcggcta ccgactcttt ggacgcactg cgtgagcgga tgcgaatttt gtttgatcct
    25681 gccgagagtt gaaaattagt tttcgttcgt ccggggcaca ggctgataat tcgtatcggc
    25741 gtgtcacgta attccgacgc gacggccacg ctgaagtggg tgacaaatgc tttcgttgca
    25801 ccatacgtcg ccataaaagg gacgggttgg aaacttgccg tggaacttac gttgaggatg
    25861 gcgcctttat tttgggagag catgccgggt aaaaaaagat gcgtgagctg gacgagcgtt
    25921 ttgatattca ggtcaatcat gttcatctgc ttagcaatat cggattcgtg aaaatatccg
    25981 tatgaaccga atccggcatt atttaccagc aaatcaacgc ggatgttaag tttgtttatt
    26041 tggtcaaata aacgttgcgg tgcgttttcc gatgtcaaat ccatcgcgat cacatctacc
    26101 gttgcaccgg atgagcgaaa ttcgcgcgca tgggtttcga gtgcatcggc cgaacgcgca
    26161 accaagatca aacgaaagcc gggtttggcc agcgctcggg ctatcgcttc accgattccg
    26221 ctggaagcgc cggtgatcaa aatcgtggtg taaggaaatt tattcacgct tgcaatctgc
    26281 ataaaccatg ttttgcaagc aagcaatttt atacggcttg ctaatgcaaa acgaatcatt
    26341 taatttccac aagcctccta aaataaatac ctatgcgttt tgtagttttt cttacaaccg
    26401 tttttgtgtt tctgaacaga ccgtcgttgg catcttttga taccaaaaaa ggtgctcggc
    26461 ccttggccat gggcggggca tatacagccg cggcgttcgg aggggatgcg ttttattata
    26521 atcccgccgg gctctggtat accgccaatc cgatgggaca agcctttttt tcacgcccct
    26581 tcaatcttac cgaacttcaa accgttacgt ttaacttcac gtatccgtcc ttttttggga
    26641 atgtgagcgc caatgccgaa tcgtatggtt acgatttata tcgtgaaacg acgttgagtc
    26701 tggcttatgc taacgtatat aaggaacgtg tggcgatggg tgttgtgata aactataacc
    26761 atcttgccat caaaaacgca ggctcggcgg cggccgtcgg attggatttt ggtattttag
    26821 ttcgaccgct gcgaatgcta accgtcggtg cgatggttcg caatatcaac catccttcga
    26881 taaaaaaaga cgcattgccg cagacattta cagtcggcgc ttgttttcaa atcatggatc
    26941 attttcgcct catggccgat gcgtataaag atgtccgtta tcccaccgat attcgtgtcg
    27001 gcggggaata catgtttatg gaaaaattta tcatacgcag cggcttaggt tccgatccgt
    27061 cccgtttctg cggcggattt ggcttggatt tagggttggc cgttttggat tatggtttta
    27121 atacgcatca ggagcttgga atgacacacg ctctgtctct cagttttcat attgaccgaa
    27181 aaccctcgcg ctcgcttcaa ccgtcattgc ggtaatcatc tatggttgtt ctgctccaac
    27241 gtatttcttt ttttatcgcg ttagtttttt attcttccgt cgtcgtcgcg caggattcgc
    27301 tatggattga taacgatcag gatcgcagca ccgaatccgc tttggagtca atgacggaga
    27361 cggcgaatga gggtgtggaa ttttcagagg cgctggaaaa ctggcggcgt aatcctttgg
    27421 atattaaccg cgcggatatt gaagaattgc aacaattacc cggattatca ccgatcttgg
    27481 ccaaagcgat cattgatcgt cggcgcgcgc aaccttttac atctatccga caacttttgc
    27541 aggtcgatgg gttttcaagg gagttgtttg tacgtattca accgtttctc atcgtgtaca
    27601 gtcataccgc ggtcaggccg tttgatgtcg cattgcgcca acgtaccgag cgccaaattg
    27661 aaaagacgcc ggagtttagc gacgggagct atgaaggcgg cccgcttaaa atgtacgaac
    27721 gtgccgttgc cgtatatgca ccgggatggt cggcgttgcc tgacgcccaa atacgcggcg
    27781 cttgggttat ggaaaaagat cccggcgaaa cccgatggaa tgaccatcag gccggttatg
    27841 ccgagataaa aaatgccggt gtagtccgtc gcgcggtggt cggtaattat cgtttggaat
    27901 ttggccaggg tgtcgcgctg tggagtggaa gcgggatgtc caaaagtacc gaagtcattc
    27961 aaagcgtaaa acgccgaagc ggagggatca aaccatttgc ttcagcaaca gaaaatcagg
    28021 ctttgcaagg tatagccgcc gaattgcaat ttgaggatgt gaatttctta cacgcactca
    28081 aactgacggg tttttattcg tatgcacact ttgatgcatc gcgcaatccg gacggaacga
    28141 ccggcggttt ggatgaaagc ggattgcatc gccagacatc ggaaaatagg cgcagggatt
    28201 atcttttaga aacattgggc ggactccatg ccgactatca aatcaacagc gctacacgat
    28261 tgggagcgac gtattatgcg catcagttta atcgccggtt tgtgcccaat gacccggtac
    28321 gcaatcgtta tgattttgaa gggcatcgca atcgagtcgg cagtatatcg tatgattatt
    28381 actatgagca tgtcaatttt ttcggcgagt gggcgatgga cggcggtgcg gataaggccg
    28441 gtaatgccgg cgtacaattt ttttggccgc acatggagtt tgtatttttt tatcgtaatt
    28501 acgctaaaaa ttttcattcc cttcgcggat atgctttcgg cgaacaaaac ggccgcacgc
    28561 gtaatgaaga aggtatttat acgggatgga aatacgaatg gcgtcgtcgc ctgaccatac
    28621 aagcgtatta cgatttgtat cgttttccgt ggcgtaccta cgatgtgccg acagccgtaa
    28681 ccgggcgcga tttcctgacg caagcggatt ttgctttgac ccgcgcaatg accatcacaa
    28741 tgctctacaa aagggaagaa aaagacgatg cgattcagag cacggatatt ttagggcgcg
    28801 atattatcat cgtcgaaccg tcacgtgtgc aacgtctgcg catacagatg gaaatgcttg
    28861 caacgccgca gtttcggttt cgttcacgga ttgaaacatc ctactacgcc ataccttcca
    28921 tgtcgggcac ttcagagaag ggtcttttga tttatgaaga tgttcgtttt cagcccgatc
    28981 gccgatggac gttgtatggt cgtgtgagcg tctttgacgc ggattccaaa tcagcggtgt
    29041 acgaatacga aaacgatgtc gaaggcgcat ttgccaatac gcgattttcc ggaaaaggat
    29101 tgcgctggta tgtcttcaca cagtatcacg gattaaatcg aatggatatc ggcttcaaat
    29161 actggcaaat ggcacgcagt gatatttcgg ggtacggtcg tatgacacgg cgggttacct
    29221 tatcggtaga ttacaaatta taaaacgccg tggcatcgga aaaaaccttt gctctttttg
    29281 acggtacggg gtatattgag ccgcttatct gcattcgcaa tacacaagct aaaatgatgt
    29341 tataacatat cgccggagga atttggagta tggaagaaaa aacgttggtc ggctatttta
    29401 cggatggtgg ttttttcatg tggccgattt tgatatgctt tatcggcggc cttgccattt
    29461 gtatcgaacg ccttataacg ctgacgcgtg cggcgaccaa taccaaacaa ttcctcgtca
    29521 aaatcaaaca atcgctggat gaaggcggtg tcgagcgtgc gacggaagta tgcgcgagcc
    29581 agcgcggatc cgtggcgttg atttttcatg cgggtctttt acgcgctcgt aaaggtgtgg
    29641 agcatgtcga aaaagcgatt gttaatgccg gcgctgtgga aatggcgttt ctggagcgcg
    29701 gtatggtatg gcttggtttt tttgtcggcg cggcacctct tttgggtttt acaggtacgg
    29761 tccaaggtat gatcgaagcg tttgatgcga tcgccaaagc taatgacatt tcacctacga
    29821 ttgtggccga cggtatccgt acggcgctat atacgacgtt attcggactt atggtggcga
    29881 ttcttttaca atttttcttc aattatttta ccgcgaaaat caatcgtatc gtggctgata
    29941 tggaagaaag ttcttcggaa ctggtggact acctgctcga aaacgaagta cggtaataat
    30001 aattttataa aattatgttt gtcgaaaaac gacgcaaata tactcctgaa atttcaaccg
    30061 cttcgatggc ggatatcgct tttcttttga tcattttttt tatcgtgatc acaaagtttg
    30121 gcaccgataa aggcattgat ctgacactgc ctccggaagg ttccattaaa gaaattccca
    30181 aaaacaatat catgagcatt acgatggatg atgccggtgc ggttgttctg gatgggaaag
    30241 cggtaattct ggggcaggtt cagtcgctcg taaaaaccat cgccgaggcc a
//
LOCUS       DAOQGF010000034        30127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_687_length_30127_cov_7.768190, whole genome shotgun sequence.
ACCESSION   DAOQGF010000034 DAOQGF010000000
VERSION     DAOQGF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_687_length_30127_cov_7.768190"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PK937_06730"
     CDS             <1..650
                     /locus_tag="PK937_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH32301.1"
                     /translation="SSLAAAGPSTPGKPRVESDSENPLTDYGKSKLAGEKIAVSFMNK
                     LPITIIRPPAVYGPREKDIFFYFQLIDKHIKPVLGFQTKYLTIIHALDLVEAIVRAAE
                     SPQSTGHTYFVDDGYVYSWQDLSSTIQKASGTWAIPLTIPEWLISGIAHIAEFFSRFS
                     SSPALLNRQKIIELKQVAWTTDSEKIKRDLSFSPNYDLLKGCKMTVDWYRENGWL"
     gene            657..1532
                     /locus_tag="PK937_06735"
     CDS             657..1532
                     /locus_tag="PK937_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH32302.1"
                     /translation="MKTFLNKINPVDWAYFIYQAVIIVMIIVFRTNLDQWASYVLWHL
                     LFILAGTSIIHFFHDTPVFALRMFRHWYVILTYAHFFIETGMLNQIVFQGFWDKSFIE
                     WDIRLFGTDPNLWFYDHLNNFWFNEIIHFCYFSYYIVPTALGVILFTQRDTNYFRLLL
                     GFTVTFYVCYFIYILTPVRGPLEMREVRFLDGGWFVQIMNWIYANAEKPGAAFPSSHV
                     AIGLLTLYYAFRYRRNLFWIFLPFMTGLVFSTVYCFYHYVVDVFAGVFVSIVMFWLCE
                     KWYAHSLKSRFPTSI"
     gene            1627..2547
                     /locus_tag="PK937_06740"
     CDS             1627..2547
                     /locus_tag="PK937_06740"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNH32303.1"
                     /translation="MRILLVPIIMLLIVSCANNASDKEGWRKIQSGTTDVLLGISMPS
                     QTTGYIVGTNGRILKTTNGGNDWSSQTSGTASFLRDVHFINDSLGVAVGESGKILYTD
                     DGGEHWNVKVSNTTSHLVHVHFANSKSGIASGQNGVILYSSNGGDTWLPRVSGTASEL
                     AGCFALSDSDFVVVGFDGTIRSSTNAGRNWVSETSGTTQHLRGISKTQNGDMIVVGYS
                     GVLLRKTSGSSEWASSTSHTTNNLFGVDCATTSGSVFVSGTSVMLESSDNGNVWHTND
                     VPTSVDLYDIYTANDGGGIAVGQNGTILIK"
     gene            complement(2604..3221)
                     /locus_tag="PK937_06745"
     CDS             complement(2604..3221)
                     /locus_tag="PK937_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNH32304.1"
                     /translation="MRKMIFSLLMMGVIATTQNINAQEARPLPDLGNGEEVDIFFFED
                     GEGFMADAPMMLDMPMMNVIMEDETGLEAEFPPMGMGDGAAEKIDLSKDQKDKIRKIR
                     GDVKKQNITLRGNLELKQIELHELLDAENPSKEQIAAKVKEIESLKTQMKMNHINARL
                     DCRNILTKEQREKMDQMRSERRDHWHEKRGHKAMKFKRFFHGGME"
     gene            3368..4099
                     /locus_tag="PK937_06750"
     CDS             3368..4099
                     /locus_tag="PK937_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH32305.1"
                     /translation="MNSANQNKLCLVTGALGHLGQALSECLLEEGYHVVGIGSGNTLN
                     ENFKKSEFLKYVSGKKTWTFYSVNLTSEEEVTQLFQSVSQNNNSLLALVNLVGGIYPW
                     SMLHEVEAKTWDAMIELNLKTTFLCSREAIKVMIPQRCGKIINIGALAGIRGNTMAGP
                     YGAAKAALINMTETMAEENKRNNIQVNAVVPGIIDTPTNRQSMPDADFSTWVKPQSIA
                     EVISFLVSEKSNALTGSVLRVTGKL"
     gene            4106..4756
                     /locus_tag="PK937_06755"
     CDS             4106..4756
                     /locus_tag="PK937_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNH32306.1"
                     /translation="MKSITDQPEYSFGEELAHAITHGIGAILSIAGLTLLVAFSALRG
                     DAWHVVSSAIFGTTLVLLYSASTLYHSFPQPKVKRIFRIMDHSAIYLLIAGTYTPFLL
                     VSLRGAWGWTLFGVVWSAAIAGVWFKVFHTGRFPKLSTTLYIVMGWVALVAVKPMMEN
                     IPLGGLILILAGGLTYTVGVVFYVMHRMIYHHAVWHLFVLGGSILHFFAVLLYVIP"
     gene            4790..7666
                     /locus_tag="PK937_06760"
     CDS             4790..7666
                     /locus_tag="PK937_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32307.1"
                     /translation="MKTRYLICHLVAFFLSSHLSAQISPLGTEQNPPGIAWKEIETKH
                     FRVVFPEEITQDAQRVATTLEYLYEPVSHSLKKKPHRITVILHNQNIISNGFVTLAPR
                     RSEWYNTPPQTAFTGTNDWYNLLSVHEFRHVVQFDKMNRGLIALMYGLFGETGTLAAS
                     FLSVPSWYWEGDATGTETALTQSGRGRLPSFNMPMRTMLLRGPQQNYYQMLLGSYKNY
                     FGGAYEMGYYMTSYVKRQHGSDMWSDILRRNSNFPFFYTFSWSMKAKTGNGAPNTFEN
                     MSQELKDLWSTQELSYKSDLTTIEVRYDNGFMNPFPNNPKTLTDGSVVFFDYNLGEAP
                     GIKIKTTDGAISHITRSTWSGNFSASQNKVIWVDPDWDLRWGLKNGSRVVVWDATTRN
                     TQTLLTNTRLTYVAMSADGQKFAAVEHSRSNKSSIILFDTQTGNIIKKLSNPNNDVII
                     NPSWSSDNKHIVYVRQTLHDGKGVTLIDIENDATTDVLPAQHTMNYTNPVIWNDWILL
                     NAHFDGVDNIFAFHKYERTWKQITHSSLGAFNPFVSAGNRLVFDEYTVDGYIANSLLL
                     DTNRWTNVDIQSRKNDGYYEPLLQQELGTIRFDSITPGTFAVKKYNSLSDLINIHSWS
                     PTLNPRQYGVSVFSQNIMSTTRLSASYNYDRNEKTSGGEVGASYGGFYPIADVTAKLG
                     SRASTYTVTTIKNGKKTEKLKWYGWNEKSLSAALRLPLNFSSQGWQRYFSASAGFSYT
                     KVSDQKISFAFDQNNGVLQSIFLSGFYYNATQPAAYVYPQWGQRIWITYSKAPFKDHY
                     NGSQFFAQGDLFFPGLLKRHSGWIQGQFERQKTGANYRYASWMIFPRGYSYIYHDAIK
                     KLGATYTVPLMYPDTDFLAMFYIKRIYGDVFGDYGVGQDYSGPNVKTKYRSAGAGGYA
                     NMHFFTLPVEFSVGYRYAYAFDKKNTENNKHAQPQIVIALTGQF"
     gene            7732..8199
                     /locus_tag="PK937_06765"
     CDS             7732..8199
                     /locus_tag="PK937_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH32308.1"
                     /translation="MSYIVWLHLILAVNSNTNDLVWHKFDQGLALAAKENKAILIDVY
                     TDWCGWCKKMDQDTYSDPRVVKYLRENFVLIRMNPETDGPVSYEGEQYPAAAFAQSMQ
                     VTGYPATAFFESNGKMITMVPGYIQSDDFLNILKYMAEKKYYTVSYEDFLKAK"
     gene            complement(8203..8757)
                     /locus_tag="PK937_06770"
     CDS             complement(8203..8757)
                     /locus_tag="PK937_06770"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNH32309.1"
                     /translation="MNLLITVSFCLMLSVLLYFITGRKLELKNRKIWLGSMIVVLLLA
                     TSIMFFAQKELDNYQAYRAWLPTPARVISAQVVGKRAFRPEIIYTYTVNGFSVTDTTD
                     MEVPGFGSKSNRFNVARNEVASVREDSTFVVYYNPANVVEHLSHIRPPHTIYIMYSTG
                     GCFFIAGTYIFLVGLFQTKKNKPD"
     gene            complement(8827..9609)
                     /locus_tag="PK937_06775"
     CDS             complement(8827..9609)
                     /locus_tag="PK937_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH32310.1"
                     /translation="MSYKNILFEVKNKIAYLTINRPDKLNALNTETFMEIMDAMKKAN
                     EDASVSGVIVTGSGEKAFVAGADISELAVQTPVTAKEFALRSEVVLCYIERMHKPVIA
                     AINGFALGGGCEFAMACHMRIASENAKFGQPEVNLGLIAGNGGTQRLPRLVGKGRAIE
                     ILCTGDMISAEDAFRIGLANRIVKKEELIPTCEKILQTIASKAPVAVKLTLEAVQSGM
                     EMTLAEGLNFEANLFGLVFSSDDMKEGTKAFLEKRKANFQGK"
     gene            complement(9629..10639)
                     /locus_tag="PK937_06780"
     CDS             complement(9629..10639)
                     /locus_tag="PK937_06780"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNH32311.1"
                     /translation="MKIAVLYGGNSSERNVSLSSGLAVISALHKMGHDVLTVDPAFGG
                     CIKEYEIKAVGELPPTEEELRDLPGSKNPHTIVEALNFISQKKVDVVFNALHGGIGEN
                     GALQAMMDVYGLRYTGSGSAASAIAMNKTLTKRIAETERVPSAAYVFLPRNTDQNQID
                     EIVRSKVSGKIVVKPNDDGSSVGLSIVDPGESLQQAFLKAASIGDVLFESFIDGRELT
                     VAVLGDQTLPVVEIKPEGGVYDYKHKYTKGLTQYFCPAEIDLVVSKKAQEFAIKAFRS
                     VGCKGYARVDFRMNQSGELFCLEINTLPGMTATSLVPKAAKAGGMEFEELVEKIIRLA
                     LP"
     gene            complement(10714..12180)
                     /locus_tag="PK937_06785"
     CDS             complement(10714..12180)
                     /locus_tag="PK937_06785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNH32312.1"
                     /translation="MEIVIPDTNITAIMPQVILTIGACVTLLMGVFTPHLRKWAGYVA
                     FATCVIAFLDVMTMWDTKMVAFSGMITLNYYTHAFSIVILASAALVCLVTYDDVSEHK
                     VGEFFSVLLMSTTGMLIMVASMNLMVIFLGLEIMSIGLYILISINRSKIRSLEAGMKY
                     FLLGAFASGFFLYGIALTYGATGSFDLARIANYLSMNNLLGDPLALAGTSLLIIGFGF
                     KVALVPFHMWSPDVYQGSPAPVTAFIASGPKVAAFAAFARVFIDTFNTLAIEWSEVLF
                     WICVISMIGGNLMALVQRDIKRMLAFSSISHAGYIIMAILAGPKLMDSNPNLAMDSIL
                     YYVMVYSLMNICAFTIIHVFETREGRNLKVDDFAGFGFRYPLLSVAMSIAMLSMAGFP
                     LTGGFIGKLRVFSAAVDAGYVALTIVAVITSTISVYYYLGVMVKMYFTDAENPLEDIY
                     LSKSTRTALALSSAGLVLLGVIPTVIADVVRTVFQAFV"
     gene            complement(12206..15556)
                     /locus_tag="PK937_06790"
     CDS             complement(12206..15556)
                     /locus_tag="PK937_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH32313.1"
                     /translation="MQSSLTTLCPNCGKELTADTQPAACPHCRYLFSVDLKSFIVPED
                     HGLCVKEIRTGIEKERFEEVGLLIHHLIHRNFLDDKALRMIGMELHATHKDALALACC
                     ERAVQINPQDAWNHHALSETCQQIGMLEAAFKHAQEAVRLDPMESRHHARIAEVYDKQ
                     NQSTLSARSYLMAMALSGAYIKNFDQLIDDAIDLVMVESTYEELRKQHAEVRIIHFAM
                     GNLYLGKMNYEKAETAFQQVISSQPSNVSALNRLAEVYQKQNKPEQALEMYKRSLASE
                     ESNLDTLRNLGKLYKEKSNWAEALKYFERILKLNNKDSNAAYETKEMYRKLNNPQKAI
                     EVMKEYQRTDVSYYNWAMHHIFEILYYDLKDDKQLIELVEKTYPNKITDSFVYDYYGY
                     SLMNTGRLDEAVKAFERFVELEPKNARVMRELGRVFCDLQRYQEAIEVLKKSFAIENK
                     NSYGYYYLALAYHKLGKDDLARKTLNEGKQFDPTYKWFDELLTEMDPNYTPTAIPENV
                     TETPVTTGTGVLGETVAPALPNFLRDLTQLAKEGKIENIFGRTHELMELIEVLCRRNK
                     ANPLIVGAPGVGKTVLVNGLAHLLAQKKVPDILQDYRLLELEVFAVVAGTRYVGTLEQ
                     KFMQLAQYCRQNKVIIFIDELHTLMGAGSYSSHETGGLDEMFKPLMTQPEFKLIGATT
                     DEEYQKNIAKSGAFDRRFTRITVFEPMRDETMNILGSLRGKIEPFYHVQVSDRMLRTT
                     YDMAEKYIKNRYFPDKACDLLERASIKASLSRSGAEGEIKEVAEEHIVQAVSQVTRIP
                     ESNIQIDAMMGMLSLEDRLRMRVIGQEEAIQKVAEVVRMTKSGLDVNPERPDGVFLFV
                     GPTGVGKTELAKALAEALEGSDKRLVRIDMSEYNDQYALSKLIGTAPGFVGYEDEGRL
                     TKAVREDPSAVILLDEIEKAHPKIYEAFLQVFDDGRMTDGKGNVISFSHTTMIMTSNL
                     GAEHIYGKEKLGFSGGTEDVHKQISQGITESMRKHFSPEFLNRIDEIIVFRPLDDEAL
                     IQIAQQKLDGIVKRFADRDIRIHIEQDVAALCVRDIDSKREGARGINRQLEDMISRPL
                     SRVMIQNPNCKAYNIKRSENEILVLRGA"
     gene            complement(15707..17293)
                     /locus_tag="PK937_06795"
     CDS             complement(15707..17293)
                     /locus_tag="PK937_06795"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNH32314.1"
                     /translation="MENYNSLALSLLIFMPLVGVVVISFIDRRNENLIKATTFAFSAL
                     VFVLSIPIYMNYDFSNPEMQFTYKAKWIVDWGAYYTLGVDGLSMLLIMLTTFMMPLAV
                     IASWKSIHFHVKGFMISLLLLDAGMVGVFCARDMLLFYVFWEAMLVPMYLIIGIWGHD
                     HRIKAAVTFFIYTMAGSLFMLVAIIYMYFQTGAVVDNGTVLSMHSFAVDDFAKLQLPY
                     ETQYWLFLAFALAFAVKVPMFPFHTWLPLAHVEAPTAGSVDLAAILLKMGPYGFMRFC
                     IPFFPEVANDFTPVIMVVAIIGIIYGALVAMVQTDAKKLVAYSSVSHLGFVMLGIFAF
                     TIQGWQGAMMIMIGHGISTSALFLMIGMLYDRRHTREIAKFGGLWKVMPAFSVIFMIV
                     CFSSLGLPGTNGFIGEFLVLIGTFISSIGHARLYATLAMLGVILGAVYLLWMYQRVCF
                     GEIVHEENKKLPDLSVREYALVTPFLILIFLLGFYPKILTEKTEATMHKYLNNQKTAV
                     EQAKTKKNSGPMMTNQPIPY"
     gene            complement(17338..19350)
                     /gene="nuoL"
                     /locus_tag="PK937_06800"
     CDS             complement(17338..19350)
                     /gene="nuoL"
                     /locus_tag="PK937_06800"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNH32315.1"
                     /translation="MEMIILMIPLIPLAGAVFNVFFGRMIEGQGSHHTEGHEEGHAHH
                     HAKPWAGYLASGLVFISFLLSLYVFIQVLGGQKYNIDYLDWIIVDTLRITFGFLIDPL
                     TGVMLLIVTGIGFLIHVYSLGYMEHDPDRPRFFTYLNLFIFSMLMLVMGNNLAVMFFG
                     WEAVGLCSYLLIGYYYKKKSAGDAGKKAFIVNRIGDFGFGLGIFLVFATFGTIHFETL
                     FDAIHKVDPYALGTTMTAIGMLLFVGAMGKSAQIPLYVWLPDAMEGPTPVSALIHAAT
                     MVTAGVFMVARCSPIFSFDIGPAGYIMGMSAGDLVTYVGAATALFAATIGFTQTDIKK
                     VLAYSTVSQLGYMFIGVGVGAYAAGVFHLLTHAFFKALMFLGSGSVIHVMEHAYHKAG
                     IHEDPQDIRKMGGLFSKAKITAITFLIGWVAICGLPPFSGFFSKDEILASAYHKGYNG
                     VFWFGVLGAMMTAFYMSRLVFLTFFGESRTPEAAKEHLHESTSTMTVPLMILAVFAAI
                     AGLIGIPPVLGGHNWLAHFLEPSVGTHAAPVEHHGFNDMTLMVISVLAGVIGIGIAWV
                     IYMAKKVSAEKIAADYQPIYKTLLNKYYIDEFYDKFIVEPTKWVSEKVFLRLVDNKLI
                     DGLIYLIAVTLDLFGRILRLFQTGYVQTYAFFISLGVALIIYYIMR"
     gene            complement(19396..19698)
                     /gene="nuoK"
                     /locus_tag="PK937_06805"
     CDS             complement(19396..19698)
                     /gene="nuoK"
                     /locus_tag="PK937_06805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNH32316.1"
                     /translation="MVPLSHYLIVTAILFSLGVVGVLTRRNAIIVFMCIELMLNSVNL
                     TMVAFARYLDSMDGQIAVFLVMTVAAAEVAVGLALIVAIYRKKDTINVDEMHMMQG"
     gene            complement(19713..20246)
                     /locus_tag="PK937_06810"
     CDS             complement(19713..20246)
                     /locus_tag="PK937_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNH32317.1"
                     /translation="MENMLQYVMVNPVFYIFAAIAVLCAIMMLTMENPVYSAIFMVGV
                     FFCIAVLYITLEAEFIAAIQVLVYTGAIMVLFLFVIMLLNLGRDAHLTEYAGPMKYIS
                     IMLAFAILVQIGLATKVTYNLGDRGIYPIEKIREAGNVEVIGSVLMSQYLYPFEIASI
                     LLLLGMVGAIIISKRKQ"
     gene            complement(20312..20923)
                     /locus_tag="PK937_06815"
     CDS             complement(20312..20923)
                     /locus_tag="PK937_06815"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNH32318.1"
                     /translation="MAVNVKNVSEPRGKSWLDNTYVIPILKGLGITFKHLFKKRDVLS
                     YPEDKKVLPPNYRGLHMLTRDENGREKCVACEMCSTACPADCIRIVANDSGDAWKDGH
                     ENREKHPKVFEIDELRCIYCGMCVEACPEDAIDMTDMHNLADYTRADFIYNKERLLGV
                     YDEYIKRHPDRAQRRDGLHKQMKNSSYFTVKDVKHSDEHGSVH"
     gene            complement(20961..21983)
                     /gene="nuoH"
                     /locus_tag="PK937_06820"
     CDS             complement(20961..21983)
                     /gene="nuoH"
                     /locus_tag="PK937_06820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNH32319.1"
                     /translation="MDAFLPFVPYLVIGLMLAIYLAIVPIMLIVERRVSAWVQYRVGP
                     NRVGPLGLFQPIADALKMMSKEDIIPLHVNKWLYVAAPALMVFPAFFVVGVIPWGFIT
                     VGGREIWIQIGQTNIGVLYVLAVSSLSVYAITFGAWASNNKYSLFGGMRSAAQMISYE
                     LPMGMAIMSMVLVVGSLKIEDIVNFQIQNHMWGIVFQPLAALLFIATAFAETNRTPFD
                     LTEAEAELVAGYHTEYSSMKFGLFYLGEYCNMLTNSIMITCLFLGGWHIPFVNDMGFS
                     PLTRDLINLGGFAFKVLFLMFFYVWVRWTLPRFRYDQLMNLGWKKMIPLALANILITA
                     IWLAFV"
     gene            complement(22012..23625)
                     /locus_tag="PK937_06825"
     CDS             complement(22012..23625)
                     /locus_tag="PK937_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH32320.1"
                     /translation="MALVKIDGQELEMQRGETILNAAKRIGIEIPYYCYHPGMKIVAS
                     CRMCLVDMVGAPKLVPGCSTTVGELPPGKKIEDKYDAVIFTENEKVTTARRAVLEFLL
                     LNHPIDCPICDKAGECKLQDYTYEHGNHESRFAEIKRIPQRKDLGPSIYLYTSRCIMC
                     TRCVRFTEEISGTEELKVNHRGARAEIEVNPGKPLNNKMMGNVVDLCPVGCLIDKNEK
                     FEGRVWNFVHTESVCPGCSKGCNTTLDTMKRQKLNLEELVDKVYRVRPRENMAVNQWW
                     ICDDGRYHLNAQEKLTRILVPHTRKDNKLSETNWTAAMALVTEKIKSAGSGSVAFIAS
                     PYASNEENYVFGKLAKSFGASLDVSKPLYGDTQTFKTDKNKYPFMIESEKAPNYRGAK
                     EVLGLGDSKMEQTIAGKKVVVLLGSHNIELKTKPETLIVISHFEDAVTNQADVVLPGT
                     IFTETSGTFTNKDGLVQKFNKAIQPAGESRPVWQIVLDIASALEYGLYYESVADITKE
                     IKETVSSYSSLDMDKLYPQLAYTNEQMVL"
     gene            complement(23699..24217)
                     /locus_tag="PK937_06830"
     CDS             complement(23699..24217)
                     /locus_tag="PK937_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH32321.1"
                     /translation="MAKAKKKVVKKAAKKVARKKVKALKKTVKKPTEKIPAKGLSPYL
                     CFNGNCREAMEFYRDSIGGKILSLQTFGDAPMDSTPEQKNLVMHSEFKADAVYFMASD
                     AMPGQPVTSGSNVTLSINFTKLAEQEAVFSKLSAGGTIIMPLQETFWGAKFGMFTDKF
                     GIQWMLNCQLKK"
     gene            complement(24236..25537)
                     /gene="nuoF"
                     /locus_tag="PK937_06835"
     CDS             complement(24236..25537)
                     /gene="nuoF"
                     /locus_tag="PK937_06835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNH32322.1"
                     /translation="MEKVLTKMVGVENSQNIDVYIKHGGYQALPKALKMTPDELIQMM
                     KDAGVRGRGGAGFPTGMKWSFLPKDNPKPRYLLCNADESEPGTFKDRVLMEDDPHQVI
                     EGLVISSFAIQANTCYIYIRGEYVKGALTLRKAIDEAYAKGYLGKNILGSGFNLDITV
                     HRGAGAYICGEETGLIESLEGKRGWPRIKPPFPAVFGAFGCPTIVNNVETLVTPVHII
                     NRGIQWWKDNEPKLYCMSGHLTRPGTYEAKMGTNLLTLINEHCGGIRHGRKLKAVIPG
                     GSSVPVLRAEECNVNMDFDSLAKVGSALGSAGCMVMDETTCMVNALWNLLKFYAHESC
                     GQCTPCREGTHWLEMILGRVETGKGRMEDLDLLLETANNIAGRTICPLGDAAAWPVAG
                     LPDKNKGGDLNGQSFVNKFREEFEYHITNKKCMTTIPHEWN"
     gene            complement(25624..26100)
                     /locus_tag="PK937_06840"
     CDS             complement(25624..26100)
                     /locus_tag="PK937_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNH32323.1"
                     /translation="MTLEFSPENKKEVEWLISRYPTKQAATLPVLHVAQQQFGWISHD
                     VMNLVAKTLDEPVSRVEDVVTFYTMFHQHEVGKYHFQVCHTLGCAINGASQVVDYLEK
                     KCGVKCGEGRSADGKFSIVKVECLGSCGTAPMLQLNDDYHENLTKEKIDALITACK"
     gene            complement(26188..27495)
                     /gene="nuoD"
                     /locus_tag="PK937_06845"
     CDS             complement(26188..27495)
                     /gene="nuoD"
                     /locus_tag="PK937_06845"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNH32324.1"
                     /translation="MEIDRSEMVEDFKDQSSEETLKKAKEAYKKHVKIGDDDESLSLP
                     GAQLLTLNFGPQHPATHGTLRMVMQLDGETIVNLDPSPGYLHTGFEKLAEHHTFNQFI
                     AVTDRMNYLSPMSNNIGFAQAAEKLLGITLTERAQYIRMIQGELSRIADHLVNIGTHA
                     LDLGAFTVFLYTFRQREFIYDLFEESTGSRLTTSYTRIGGVVRDFPEDHPRHIAQWCK
                     QFKATCTDQVEGLLNHNKIFLDRTQNVGKISAEDAINCGMTGPCLRASGVATDIRKDE
                     PYLKYDEVDFDVPIGTEGDVYERYLVRMEEMKQSIRIIEQCLERMKPGPVKVADHTIA
                     LPDKNKVYGSIEGLIHHFKLIMDQHGIAMPKGEIYDATEAPNGELGWYIVSNGEGTPY
                     KVHVRAPSFIHFGMMSHMAKGGLIGDAVAILGSMNVIAGECDR"
     gene            complement(27549..28070)
                     /locus_tag="PK937_06850"
     CDS             complement(27549..28070)
                     /locus_tag="PK937_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNH32325.1"
                     /translation="MPNNEKNILENNPTLDAVKSKFGESLLKSEVFRNELTLYIKRES
                     IAEIMAFLKNDSDLAYDMLNDVTVVDGLIMEKNPRFQVVYHLRSTKYNRRLRIKAPVP
                     ENDCKIATVSHLWKLADWAEREAYEMYGVTFEGHPDLRRLLTPASFTSFPLRKDYPLR
                     GKGERDVILPEGS"
     gene            complement(28112..28504)
                     /locus_tag="PK937_06855"
     CDS             complement(28112..28504)
                     /locus_tag="PK937_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNH32326.1"
                     /translation="MLFILQDGPVYLNYLPVLVLMALAIIIPIAILILSRLLGPYKPN
                     ERKLSVYESGMPPVGDAHPKFSVKFYLIAMLFILFDIEIVFMYPWAVIYNKWVSVSTF
                     ILIEGLVFIGILLVGYAYAWKKGALEWE"
     gene            complement(28737..>30127)
                     /locus_tag="PK937_06860"
     CDS             complement(28737..>30127)
                     /locus_tag="PK937_06860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNH32327.1"
                     /translation="RIDMTYPSYVPFMKYSQSESARKALYVKYNNRSVDKNLHVLRDL
                     VGQRQEMATLLGYKTYGQYRVEDRMAKKPENVWNFESALIDKVKEKARRDYDELLAVK
                     RNYLKDASASVINPWESSFYNGILMREKYSVDDEKIKEYFELNNVTEGLFKISQKLFD
                     VEFSEIKNPSVWHSDVRMFEVRKKGKVIAHFYFDLFPRPNKYGHAACFGMVKGKSTPN
                     GYQLPAATLECNFPAPSGDRPSLMYHSAGSASVETFFHEFGHVLHNILTTSELYSQSG
                     TAVPRDFVEAPSQIFENWAWDYDVVKMFAKHYKTGEVLPKAMFDKMVAAKNVGSGIGA
                     LQQVFYGMVDFTLHEKWNPKGAETSTDIVKRLQNEITLFPYLEGTNFQASFGHLNGYG
                     AGYYGYLWSEVYAADMFSVFEKNGVMDQKTGLRYRDIILARGGSEDPYALVKEFLGRE
                     PNQEAFLKSLGL"
BASE COUNT         8496 a         7023 c         6097 g         8511 t
ORIGIN      
        1 tcagcagttt agccgcggcc ggcccttcta cacccggcaa accacgcgtc gaatcagact
       61 ctgaaaaccc gctgacggac tatggtaaaa gtaaactggc aggtgaaaaa atagcagtat
      121 cttttatgaa taaactcccg attaccatta ttcgcccacc ggctgtgtat ggtccaaggg
      181 aaaaagatat tttcttttat tttcaactga ttgataaaca catcaagcct gtacttggtt
      241 ttcaaacaaa atatctcacg attattcacg ccttggattt ggtagaagct atcgttcgtg
      301 ctgccgagtc gcctcaaagc accggccaca catatttcgt tgatgacgga tatgtatatt
      361 catggcaaga cttgagttca acgatccaaa aagcctccgg tacatgggcc attcctttga
      421 ccatcccgga gtggctgatc agcggaatcg ctcacatcgc agaatttttt tctcgatttt
      481 cttcaagccc tgctcttttg aatcgtcaaa aaataatcga actcaaacaa gtggcgtgga
      541 ccactgattc cgaaaaaata aaaagggatt taagcttctc cccaaattac gaccttttaa
      601 agggatgcaa gatgacggtt gactggtatc gtgaaaatgg atggttataa taaaaaatga
      661 aaaccttttt aaataaaatt aacccggtgg attgggccta ttttatttat caggctgtaa
      721 tcatcgtgat gattattgtc tttcggacta atcttgatca atgggcatct tatgttttgt
      781 ggcatttatt atttatttta gccgggacat caatcattca tttttttcac gatacgcctg
      841 tctttgctct gcgtatgttt aggcattggt atgtcatctt gacttatgct cattttttta
      901 ttgaaaccgg catgctcaac caaattgtat ttcaggggtt ttgggataag tcatttattg
      961 aatgggatat tcgattgttc ggcacagatc ccaatttatg gttttatgat cacttgaata
     1021 atttttggtt caacgaaatc attcattttt gctatttttc atattatatc gttccgacag
     1081 cgctcggcgt tattttattt actcagcgcg ataccaacta ttttcgtctg ttattgggtt
     1141 ttacggtcac tttttatgtg tgttatttta tatacatact cacaccggta cgcggccctc
     1201 tggaaatgcg cgaagttcgg tttttagacg gcggatggtt tgtacaaatt atgaattgga
     1261 tatacgccaa tgcagaaaaa cccggagctg catttcccag ctcccatgtt gcgatcggtt
     1321 tacttacttt atattatgcg tttcgctacc ggcgaaatct tttctggatt tttttaccgt
     1381 ttatgacagg attggttttt tctacggttt attgttttta tcactacgtc gtagatgtct
     1441 ttgccggtgt ttttgtcagc attgttatgt tttggttatg tgaaaaatgg tatgcgcatt
     1501 cgttaaaatc ccgttttcct acgtcaatct aactttttta atcccgctct atacattaag
     1561 tgaaaccttc catagtgacg tacgtatcct gatttaatcc tttctcgtct taccaaagga
     1621 aatcctatgc gtatccttct tgtaccgatt atcatgctat taattgttag ttgtgcaaac
     1681 aatgcatctg ataaagaagg atggcgaaag attcaatcgg gcactaccga cgtgcttcta
     1741 ggaatatcca tgccttccca aactacgggg tacattgtcg gaacaaatgg tcgtatttta
     1801 aaaacaacca acggcggaaa tgattggtca tcgcaaacca gcggtacggc atcatttctg
     1861 cgggatgttc attttataaa tgattcgctt ggtgtagccg taggtgaatc cggaaaaata
     1921 ttatatacag acgacggcgg agaacattgg aacgttaaag ttagtaatac gacttcacat
     1981 ttagtgcatg ttcattttgc aaattcaaaa tccggtattg catcaggtca aaatggtgtg
     2041 attttatatt cctccaacgg aggtgatact tggctgccac gcgtcagcgg aaccgcatcc
     2101 gaattggccg gatgttttgc attaagtgac tccgattttg tcgtggtcgg ctttgacgga
     2161 acgatacgca gcagtacgaa tgccggtcgc aactgggtaa gcgaaacaag cggaacaacc
     2221 caacatctgc gcggcattag caaaacccaa aacggggaca tgatagtcgt cggatacagc
     2281 ggtgtcctac ttagaaaaac ttccggttca tcggaatggg catcctccac aagccatacg
     2341 accaataatc tttttggtgt ggattgtgct acaacttcag ggtcagtttt tgtgtcaggc
     2401 acatcggtaa tgttagaaag ttccgataac ggtaatgtat ggcataccaa tgacgttcca
     2461 acctctgtcg atctatatga tatttatacc gcaaatgacg ggggaggtat agcagtgggt
     2521 caaaacggaa ctattctaat caaataaaaa aaagcgaaac cattttcacg gtttcgctat
     2581 accagaagag agggcggtta tttttattcc ataccgccat ggaaaaagcg tttgaatttc
     2641 attgctttgt gaccacgctt ttcgtgccag tgatcgcgtc tttcggaacg catttgatcc
     2701 attttttcac gttgttcttt ggtcaaaata ttgcggcaat ccaaccgtgc attgatatga
     2761 ttcattttca tttgcgtttt taagctctcg atttctttta ctttggctgc aatctgttct
     2821 tttgaaggat tttcagcatc caaaagttca tgcaactcga tttgcttcag ctcaagattt
     2881 ccgcgcaacg tgatattttg tttttttaca tcgcctctga tttttcggat tttatctttt
     2941 tgatctttgg ataaatctat tttttcggcc gcaccgtcac ccatacccat cggcgggaac
     3001 tctgcttcaa gacctgtctc gtcttccata atgacattca tcatgggcat atcaagcatc
     3061 atcggtgcat ccgccataaa accttctcca tcttcaaaaa agaagatatc aacttcttcg
     3121 ccgttcccta gatccggcaa tggtcttgcc tcttgtgcat tgatattttg agttgtagct
     3181 atcaccccca tcattagcaa actgaaaatc atctttctca taaataaact ccttaatttt
     3241 gttttaatta catgtattgg acgaatgccg ttaaaattgg tttaataccg aactgtcatt
     3301 taattgggat ggtggttcgt aaaattactt attggatttg tcagttttaa tattgagttt
     3361 atctataatg aattctgcta atcaaaataa attgtgtctg gttacgggcg cattaggtca
     3421 cttagggcaa gcgctaagtg aatgccttct tgaagaaggt tatcacgtag taggcatcgg
     3481 ctcgggtaac acactaaatg aaaattttaa aaaatccgaa tttttgaaat atgtatccgg
     3541 aaaaaaaact tggacatttt actcagttaa cctgaccagc gaggaggaag tcactcaatt
     3601 attccaatct gtctcccaaa ataacaattc gttattggct ttagttaacc ttgtcggcgg
     3661 tatttatccg tggtctatgt tgcatgaagt tgaagccaaa acatgggacg caatgatcga
     3721 acttaacctc aaaacgactt ttttatgttc gcgggaagcc atcaaagtaa tgattcctca
     3781 acgttgtggt aaaataatta acatcggcgc gctcgccggt ataaggggta atacgatggc
     3841 cggaccttat ggcgcggcca aagccgctct tatcaatatg acggaaacta tggctgaaga
     3901 gaacaaaagg aataatattc aggtcaacgc cgtcgtaccc ggcattatag atacaccgac
     3961 caatcgtcag tctatgcctg atgcagattt tagtacgtgg gtaaaacctc aatctatagc
     4021 cgaagtgata tcttttttag tctctgaaaa atctaacgca ctaacagggt ctgttttgcg
     4081 tgtaaccgga aaattataaa cgcctatgaa atctataacc gatcaacctg aatattcttt
     4141 tggtgaagaa ttagcccatg ctatcacaca tggtatcggc gctattctca gtattgccgg
     4201 cttgacattg cttgtcgctt tttccgcatt acgcggtgat gcatggcatg tcgtatcttc
     4261 ggcaattttc ggaacaacat tggttcttct gtattcagca tcgacactgt atcatagttt
     4321 cccacaaccc aaggtaaaac gaatttttcg tatcatggac cactcggcga tttatttatt
     4381 gatcgccggt acgtataccc cttttttact cgtgagtctt cgtggcgcct ggggatggac
     4441 actgtttggt gttgtatgga gcgcagccat agccggtgtg tggtttaaag tgtttcatac
     4501 cggaagattc cccaaacttt cgacaacatt atacattgtc atgggttggg tcgctctggt
     4561 tgccgtaaaa ccgatgatgg aaaatattcc cttgggcggt ctgatactca tactggccgg
     4621 cgggcttacc tataccgtgg gcgtagtatt ttatgtcatg catcgcatga tctatcacca
     4681 cgcagtttgg catttgtttg ttttaggcgg gagtattttg cacttttttg cggttctttt
     4741 atatgttatt ccctaagatc ctctgtttca tctataatct attcgtacta tgaaaacccg
     4801 ttatctcatc tgtcatctcg ttgctttttt tttgagttca catctatcgg ctcaaatttc
     4861 acctttggga accgaacaaa atccgcccgg cattgcatgg aaagaaattg aaacgaaaca
     4921 ctttcgcgtt gtttttcctg aagaaataac ccaagatgca caacgtgtag ccacaacact
     4981 tgagtatttg tatgaaccgg tttcccattc attaaagaag aaaccgcata gaatcaccgt
     5041 tattcttcac aatcaaaata ttatttcaaa cggatttgtg acgcttgcac ctcgacggag
     5101 cgaatggtac aacacgccac ctcaaactgc ttttaccgga accaatgatt ggtataatct
     5161 tctttcggtt catgagtttc gtcatgttgt tcaatttgat aaaatgaatc gcggtcttat
     5221 agctttgatg tatggattat tcggcgaaac aggaactttg gccgcaagct ttttgagtgt
     5281 accgtcatgg tattgggaag gtgatgcgac aggaacggaa accgcactaa cacaaagcgg
     5341 gcgcggacgc cttccttcgt ttaatatgcc gatgcgaact atgttattgc gcggcccaca
     5401 gcaaaattat tatcagatgc ttttgggttc ctacaaaaac tatttcggcg gcgcttacga
     5461 aatgggctat tatatgacgt cgtacgtcaa gcgccagcac ggatccgata tgtggtctga
     5521 tattcttcgc cgcaattcca attttccgtt tttttatact ttttcctggt ctatgaaagc
     5581 caaaacgggg aacggtgcgc cgaatacatt tgaaaatatg tcacaagaac tcaaagattt
     5641 gtggtcaact caagagctgt cttacaaatc agacctgaca acgatcgagg ttcgctacga
     5701 caatggattt atgaatcctt tcccaaacaa tccaaaaact ttgacggacg gttctgtcgt
     5761 tttttttgat tacaatctcg gtgaagcccc cggcataaaa ataaaaacga cagatggagc
     5821 tatttctcat attacgcgat caacatggtc tggtaatttc agcgcatcac aaaacaaagt
     5881 tatttgggtt gaccctgatt gggatctgcg atggggattg aaaaacggta gccgcgttgt
     5941 cgtgtgggat gccactactc gaaatactca aactctttta actaacacac gactcaccta
     6001 tgttgcgatg tctgccgacg gacaaaaatt tgccgctgtg gaacacagcc gtagcaataa
     6061 gtcttcaata atattgtttg atacgcaaac aggtaacatt ataaaaaaac tttccaaccc
     6121 taataacgat gttatcatca atccgtcttg gtcttccgac aacaagcata tcgtttatgt
     6181 acgtcaaacc ctgcacgatg gaaaaggtgt tacccttatt gatatcgaaa atgacgccac
     6241 gaccgatgtg ctacccgctc aacatacgat gaactatact aatcctgtca tttggaacga
     6301 ctggatactt ttgaatgctc actttgacgg ggtggataat atttttgcct ttcataaata
     6361 cgaacgcaca tggaaacaaa tcacgcacag ttcgttaggc gcgttcaatc cgtttgtttc
     6421 cgctggtaat agattagtat tcgatgaata tacagtagac ggttacattg ctaattcttt
     6481 acttttggat accaatcgct ggacaaacgt agatatccag tcccgtaaaa atgacggtta
     6541 ttatgaaccc ttgcttcaac aagaacttgg aaccatccgt tttgatagca taacaccggg
     6601 taccttcgcc gtaaaaaaat acaactctct atccgatcta atcaacatac attcatggtc
     6661 gcctacactg aatcccagac aatatggtgt atccgtattt tcccaaaaca tcatgtccac
     6721 gacacgtttg tcggcatctt acaattatga ccgcaatgaa aaaacatccg gcggtgaagt
     6781 gggcgcatcc tacggtggct tttatccgat tgcggatgtt accgccaaac tcggttcgcg
     6841 tgcgtctaca tataccgtta ctactatcaa aaacggtaaa aaaactgaaa aactgaagtg
     6901 gtacggatgg aatgaaaaaa gcttaagtgc agcattgagg ttaccactca atttttcgag
     6961 ccaaggatgg caacgttatt tttcagcatc agccggtttt tcgtatacaa aagtttccga
     7021 ccaaaaaatc tctttcgcat ttgatcaaaa caatggcgtt ttgcaaagta tctttctttc
     7081 cggcttctat tataacgcaa cacaaccggc cgcttatgtc tatccgcagt ggggtcagcg
     7141 catttggata acgtatagca aagcgccttt taaagatcat tataacggga gccaattttt
     7201 tgcccaaggt gatctgtttt tcccgggttt actaaaacgc cacagcggat ggatacaagg
     7261 tcaatttgaa agacagaaaa ccggagccaa ctacagatac gcaagctgga tgatttttcc
     7321 gcgcggttat tcgtacattt accacgatgc tattaagaag cttggggcta cttataccgt
     7381 tccgcttatg tatcctgata cggatttttt agccatgttt tatatcaaac ggatttatgg
     7441 ggatgttttt ggtgactacg gagtcgggca agattattcc ggacccaacg tcaaaaccaa
     7501 atatcggtct gccggggcgg gaggatatgc caacatgcat ttttttacct taccggtcga
     7561 atttagcgtc ggttatcgct atgcatatgc ctttgataaa aagaacactg aaaataacaa
     7621 acatgctcag cctcaaattg taatcgcgct cacgggacaa ttttagtttt attaacagga
     7681 atacattatc gatcatttcg ttctaatcta agaatcattt aaagaggatc tatgagttat
     7741 atagtttggc ttcatttaat tttggctgtg aattctaaca caaatgattt ggtttggcat
     7801 aaatttgatc aaggtcttgc tttagcggct aaagaaaaca aagctatact gattgatgtt
     7861 tatacggatt ggtgcggatg gtgtaaaaaa atggatcagg atacctattc cgatccgcgt
     7921 gttgtaaaat acctacgcga aaattttgtg ctgatacgaa tgaaccctga aacggacgga
     7981 cctgtgagct acgaaggtga acaatatccg gcggcggctt ttgcgcaaag tatgcaggta
     8041 acgggctacc cggctacagc atttttcgaa tccaacggga aaatgatcac gatggtaccg
     8101 ggatatattc aatccgatga ttttttgaat attttaaaat atatggccga aaaaaaatat
     8161 tacactgtca gttacgaaga ttttttgaaa gccaaatgat gcttaatccg gtttattttt
     8221 tttagtttga aataatccga caagaaaaat ataggttccg gctataaaaa agcaccctcc
     8281 ggttgaatac attatataga tcgtatgggg aggacgtata tgtgaaaggt gttcaaccac
     8341 atttgccgga ttataataaa caacaaacgt cgaatcttct ctcacggaag ctacttcatt
     8401 tcttgctacg ttaaaccggt tagatttgct cccaaatccg ggaacttcca tatcggtcgt
     8461 atcagttacc gaaaaaccgt ttaccgtata tgtataaata atttccggcc tgaaagccct
     8521 tttccctaca acttgcgcag aaataacacg tgccggcgtc ggcaaccacg cccgatacgc
     8581 ctgatagtta tccaattcct tttgcgcaaa aaacataata cttgttgcca acaggagaac
     8641 gacgatcata ctgccaagcc atatttttct attttttaat tccaacttac ggccggtaat
     8701 aaaatacaga agcactgata acatcagaca aaacgatact gttattagta aattcatcgt
     8761 ccacctaaca aaaactgccg caatttatgt gcaactgcgg cagttttaac atattgaatg
     8821 tgttgtttat ttaccctgaa aattggcttt tcgtttttcc aaaaacgcct tggttccttc
     8881 cttcatatca tcgctgctga atacaagtcc gaataaattg gcctcaaaat taagaccttc
     8941 agccaatgtc atttccattc cgctttgtac ggcttccagc gtgagtttca cggctaccgg
     9001 tgctttcgaa gcgatcgttt gcaggatttt ttcgcacgtc ggtatcagtt cttctttttt
     9061 gactatacga tttgccaagc ctatccggaa tgcatcttca gctgaaatca tatcgcctgt
     9121 acaaagaatt tcgatcgcac gccctttgcc gacaaggcgc ggtaaacgtt gcgtaccgcc
     9181 attaccggcg atgagaccga gattcacttc cggttgccca aattttgcat tttcggaagc
     9241 gatgcgcatg tggcatgcca tcgcaaattc acatccgccg cctagcgcaa aaccgttgat
     9301 agcggcgata accggtttgt gcatccgttc gatgtaacaa agaaccactt cgcttcggag
     9361 ggcaaattct ttggccgtta ccggtgtttg tacggcaagt tcagaaatat ccgcgccggc
     9421 aacaaacgct ttttctccgc tacccgtaac gatgacgccg cttacggagg catcctcatt
     9481 ggcttttttc atcgcatcca tgatttccat gaatgtttcc gtattcaatg catttagttt
     9541 atccggcctg ttaatagtca gataagcaat cttgtttttt acttcgaata aaatgttttt
     9601 gtaagacatg gctcaactcc gagtgaattt atggtaatgc taatcgaata attttttcta
     9661 ctaattcctc aaattccatc ccccccgctt tcgccgcttt gggtacaagg ctcgtggcgg
     9721 tcattccggg caaagtgttt atttctaaac aaaaaagttc accggattga ttcattctaa
     9781 aatctacccg tgcataacct ttgcatccga cggatcggaa tgcttttatt gcaaattctt
     9841 gcgccttttt tgaaacaacc agatctattt ccgccgggca aaaatactgg gtaagacctt
     9901 ttgtgtattt gtgtttataa tcatacacac caccctcggg tttgatctct acgactggca
     9961 atgtctgatc accaagaact gcgaccgtca actctctacc gtcaataaaa ctttcaaata
    10021 aaacatcgcc gatggatgct gctttcagaa atgcctgctg caatgattca ccgggatcta
    10081 cgatactgag gcctaccgaa gatccgtcgt cattgggttt gactactatt ttaccggaaa
    10141 cttttgatcg tacaatttca tctatttgat tttgatcggt attccgcggc aaaaatacat
    10201 atgcagccga aggcaccctt tccgtttccg caatacgctt ggtgagcgtt ttattcatgg
    10261 ctatcgcgct tgccgctgaa ccggagccgg tatatctcaa accataaaca tccatcatgg
    10321 cctgcaaggc gccattttct ccgataccgc catggagcgc attaaaaacg acatcaactt
    10381 ttttttggct gataaaattt aatgcttcga caatcgtatg tggattttta cttcccggta
    10441 aatcgcgcaa ttcctcttca gtcggtggca attctcctac ggctttaatt tcgtattcct
    10501 ttatacaccc gccaaatgcc ggatctaccg ttagtacgtc atgacccatt ttatgaagtg
    10561 cggaaattac cgcaagacca gaactcagcg atacatttcg ttcgctggaa ttacctccat
    10621 ataataccgc aattttcatc gttttgcgtc gttaaaaaaa atagttctca tcgcttggat
    10681 gagaactatt gaatggcatt tcatattttt tatttaaaca aaagcttgaa acactgtacg
    10741 taccacgtca gcgatcaccg tcggtatcac gccaagcaat acaagcccgg cgctgcttag
    10801 tgcgagcgct gtgcgtgtgg acttggaaag ataaatatct tcaagcgggt tttctgcatc
    10861 cgtaaaatac attttgacca taacaccgag ataataatat accgaaatcg tagaagtgat
    10921 aacagcgacg atcgtcaagg ccacataacc tgcatccaca gctgccgaga acactcttaa
    10981 ctttcctata aatccgccgg taagagggaa tccggccata gataacattg caatggacat
    11041 cgcaacgctt aacaggggat agcgaaatcc aaacccggca aaatcatcta ccttgaggtt
    11101 tcgaccttca cgtgtttcaa aaacgtgaat gattgtaaac gcacatatat tcatcagcga
    11161 ataaaccatg acgtagtaga gtatcgaatc catcgctagg ttaggattac tatccattaa
    11221 cttcggtccg gcgagtatag ccatgatgat atatccggca tgtgaaatac tcgaaaatgc
    11281 aagcatacgt ttgatatcgc gttgtaccaa tgccatcagg tttccgccga tcatgctgat
    11341 cacacagatc caaaaaagca cttcagacca ttcgatcgct aacgtattga aggtgtcaat
    11401 gaatacacgc gcaaaagctg cgaatgcggc cactttggga ccactcgcga tgaatgctgt
    11461 taccggagcg ggcgaacctt gatatacatc gggcgaccac atgtgaaatg gaactaacgc
    11521 aaccttaaaa ccgaaaccaa taattaatag cgaagtgccc gccaacgcca gaggatcacc
    11581 gagtaaatta ttcattgaaa gataattagc aattcgtgca aggtcaaaac tacccgttgc
    11641 tccgtacgtc aatgcaatac cataaagaaa aaagccggat gcaaaagcgc caagtaaaaa
    11701 atacttcata ccggcttcca gcgaacgaat tttagaccgg ttaatactga taaggatata
    11761 taaacctatg gacatgatct caagcccgag gaaaatcacc atcaggttca tacttgccac
    11821 catgatcagc atgcccgtcg ttgacatcag caacaccgaa aagaattcgc ctactttatg
    11881 ttcactcacg tcgtcgtatg tcacgaggca aactagcgca gcgctcgcga gtatgacgat
    11941 actgaaagca tgcgtgtaat agtttagcgt tatcataccg ctaaaagcta ccattttggt
    12001 atcccacatc gtcatcacat caagaaatgc gatcacacat gtcgcaaacg cgacataacc
    12061 cgcccatttt ctcagatggg gtgtaaaaac acccatcaat aatgttacgc atgcgccgat
    12121 ggtcaggatc acctgcggca tgatcgctgt tatattcgta tccggaatga ctatttccac
    12181 aaatcctccg taaggttttt atattttatg cgccgcgcaa aacgagtatt tcgttttcac
    12241 tgcgtttgat gttataagct ttacaattcg ggttttgaat catgacacgt gacagcggtc
    12301 tggatatcat gtcttccaat tgacgattta ttccgcgcgc gccttcccgt ttgctgtcga
    12361 tgtctcgcac gcacagcgcc gcaacatctt gttcgatatg gatgcgaata tctcggtcgg
    12421 caaaacgttt gactattcca tccaacttct gctgcgcgat ttgtataagc gcttcatcat
    12481 ccaatggcct aaacactata atttcgtcaa ttcgattcaa aaattcaggt gaaaaatgct
    12541 ttcgcatgga ttctgtgata ccttgcgaaa tttgcttgtg aacatcttct gttccacccg
    12601 aaaaaccaag tttctctttt ccataaatat gttcggcacc gagattggat gtcataatca
    12661 tcgtcgtatg agaaaacgaa atcacatttc ctttaccatc cgtcatacga ccgtcatcaa
    12721 aaacctgcaa aaacgcttca taaatttttg ggtgcgcttt ttcgatttcg tccaaaagta
    12781 ttaccgccga aggatcttcg cgcacagctt tagttaaccg cccttcgtct tcatacccta
    12841 caaaaccggg tgctgttccg atcagtttgg ataaggcata ctgatcatta tactccgaca
    12901 tatcaatgcg aacaagtctt ttatcactgc cttctaacgc ttcggccaat gctttagcca
    12961 attcggtttt tccgacgccg gtcgggccta caaaaagaaa aacaccgtcc ggtcgttccg
    13021 gatttacatc aagaccgctt ttggtcatgc gtacgacttc agcgactttt tggatcgctt
    13081 cctcttgccc tatcacccgc atgcgcaaac gatcttcgag cgacagcatg cccatcatgg
    13141 cgtcaatttg aatattactt tcgggaatgc gtgttacttg agaaaccgct tgtacgatgt
    13201 gctcttcggc aacttctttt atttcacctt cggcgcccga acgcgacaga cttgctttga
    13261 tcgaagcgcg ctcaagtaag tcacaggcct tatccggaaa gtaacggttt ttgatatatt
    13321 tttcggccat atcgtacgtc gtacggagca tacgatcact gacttgcaca tgataaaacg
    13381 gttcgatttt tccgcgcaaa gatccgagta tattcatcgt ctcatcacgc ataggttcaa
    13441 atactgtaat acgtgtaaaa cgacgatcaa aagccccgct tttagctatg tttttctgat
    13501 attcttcgtc cgtcgtcgca ccgatcaatt tgaattccgg ttgcgtcatc agcggtttga
    13561 acatttcatc caacccgcct gtttcatgcg aactatagga accggcgccc atcaatgtgt
    13621 gcagctcatc gataaaaata attactttgt tctgccggca atattgagcc agttgcataa
    13681 atttctgctc aagcgttccg acatatcgtg tacccgccac aacggcaaaa acttccagtt
    13741 cgagcaaacg gtaatcttgc aaaatatcgg gaactttttt ttgcgccagt aagtgagcaa
    13801 gaccattcac caaaaccgtt ttaccgacgc ccggtgcgcc tacgatcaac ggattagctt
    13861 tattccgacg gcacaaaact tcgatcagtt ccatcaactc atgcgtgcgg ccaaaaatgt
    13921 tttcgatctt cccttctttg gccaattgcg tcaggtcgcg taaaaaattc ggaagcgccg
    13981 gcgccaccgt ttcacccaaa acgcccgtac cggtcgtaac cggtgtttcg gttacatttt
    14041 ccgggattgc cgtcggcgta taattggggt ccatctctgt cagaagttca tcaaaccatt
    14101 tatacgttgg atcaaactgt ttgccctcat tcaatgtctt tcgggccaga tcatccttgc
    14161 ctaacttgtg ataggctaag gcgaggtaat aatagccgta cgaattctta ttttcgatgg
    14221 caaatgattt ttttaaaact tctatagctt cctggtagcg ttgcaaatcg cagaatacac
    14281 gccccaattc gcgcatgact cgtgcatttt taggctcgag ttcgacaaaa cgttcaaacg
    14341 cttttacggc ttcatccaat cgccccgtat tcatcaaact atatccataa taatcgtaaa
    14401 caaacgaatc ggtgatttta ttgggatacg ttttttcgac gagttcgata agttgtttat
    14461 cgtctttcag atcatagtag agaatttcaa aaatatgatg catcgcccaa ttatagtagg
    14521 acacatccgt gcgctgatac tctttcatta cctcaatagc tttttgagga ttattgagtt
    14581 tgcgatacat ttctttggtt tcgtacgctg cattactgtc tttattatta agcttaagaa
    14641 tgcgttcaaa atatttcagc gcttccgccc aattgctttt ttctttatac agttttccga
    14701 gattacgcaa cgtgtctaaa ttggattctt ccgatgcgag gctgcgtttg tacatttcaa
    14761 gcgcttgttc gggtttattt tgtttttgat aaacctccgc aagacggttt aatgcagaaa
    14821 cattggaagg ctgcgatgaa atgacttgct gaaacgcggt ttccgctttt tcgtaattca
    14881 tctttccgag atacagattg cccatcgcga agtggattat ccgtacctcc gcatgttgtt
    14941 tgcgaagctc ttcgtaggtg gattccacca taacaagatc tatcgcatca tcaatcaatt
    15001 gatcaaagtt tttgatatac gcaccgctaa gggccattgc cataagatag gagcgtgcac
    15061 tgagcgtaga ttgattttgt ttgtcataca cttcagcgat acgcgcatga tggcgtgact
    15121 ccataggatc caaccgaaca gcttcttggg catgtttgaa tgccgcctca agcataccga
    15181 tctgctggca tgtctccgaa agggcgtgat gattccacgc atcttgcgga ttgatctgta
    15241 cggcacgttc gcagcacgca agcgctagtg catccttatg cgtcgcatgc agttccatac
    15301 cgatcatacg cagcgcttta tcatccaaaa aattgcgatg tataaggtga tgaataagaa
    15361 gcccgacttc ttcaaaacgt tctttttcga tgccggtgcg gatttctttg acacacaacc
    15421 cgtgatcttc cggtacgata aaacttttca aatccacact aaacaagtaa cgacaatgcg
    15481 ggcatgcggc cggctgcgta tccgccgtca gttctttacc gcaattgggg cacaatgtgg
    15541 ttagtgaaga ttgcattgga agttactact gagtttctag ttgttcaaag ttttatacaa
    15601 aaaaggttcg cagcatgcgc gaaccttttc ataattggtc gaaatcactg cttcatgcgc
    15661 tatcgtcatt tgtttatcat tcgcacaaag ccgcatgcaa aagatattag tacggtatcg
    15721 gctgattggt catcatcggg ccgctatttt ttttggtttt ggcctgttcg acagccgttt
    15781 tctgattatt cagatactta tgcatggttg cttctgtttt ttcggtcaga attttcggat
    15841 agaatcccaa caggaaaatc agaataagaa acggtgtgac aagtgcatat tcacgtactg
    15901 aaagatcggg aagttttttg ttttcctcgt gaacgatctc gccaaagcag acgcgttggt
    15961 acatccaaag aagataaaca gcgcccaaga tcacgcccaa catagccagc gtcgcataga
    16021 ggcgggcatg gcctatggac gaaataaatg ttccgatcag aacaaggaat tcaccgataa
    16081 acccgttggt tcccggaaga cccaatgatg aaaaacaaac aatcataaaa ataactgaaa
    16141 aggccggcat gactttccac aaaccgccaa atttcgcaat ttcgcgtgtg tgacgacgat
    16201 cgtataacat accgatcata agaaagagtg cgctggttga tataccgtga ccgatcataa
    16261 tcatcatcgc gccctgccaa ccttgaatcg taaaagcaaa aataccgagc ataacaaaac
    16321 caaggtggct tacggatgaa tacgcaacca attttttggc gtcggtttgt accatagcga
    16381 caagtgcgcc ataaataata ccgatgatcg ctaccaccat aatcacgggc gtaaaatcat
    16441 tggcgacttc aggaaaaaac ggaatacaga aacgcataaa accgtagggc cccatcttta
    16501 acaagatcgc ggctagatcc actgagcctg ccgtcggcgc ttcgacgtgg gccaagggca
    16561 accatgtatg aaaaggaaac atcggaactt taactgcgaa ggcgagtgca aacgcaagga
    16621 ataaccaata ttgagtctcg tatggtaatt gtaattttgc aaaatcatca acagcaaacg
    16681 aatgcatgga gagtacggtt ccattatcca ctactgcacc ggtttggaaa tacatataaa
    16741 taatcgcgac gagcatgaac aagctgccgg ccatagtata tatgaagaac gtcaccgcgg
    16801 ctttgatacg atgatcgtgg ccccaaatac cgatgatgag atacatcggc accagcatag
    16861 cttcccagaa cacgtaaaac aaaagcatat cccgcgcaca aaaaacaccc accattccgg
    16921 catccagtaa aagcaacgaa atcatgaaac ctttaacgtg aaagtgaatg cttttccatg
    16981 aagcaatcac agccaaaggc atcataaaag tcgtcagcat gatcaaaagc atcgaaagac
    17041 cgtccacgcc caaagtatag taggcccccc aatcaacaat ccattttgct ttatacgtaa
    17101 actgcatttc cggattgctg aagtcgtaat tcatgtagat cggtatggac aaaacaaaca
    17161 cgagcgcaga aaacgcaaat gttgttgctt tgatcaagtt ttcgttgcgt cggtcaatga
    17221 aagaaataac gacgacaccg accaacggca taaaaatcag aagtgaaagc gcaagcgaat
    17281 tataattttc catggaaaag cctctatcaa atcaacgaac ttttttcaga attacctcta
    17341 gcgcatgata tagtaaataa tcagcgccac acccagcgaa ataaaaaatg catacgtctg
    17401 aacataaccg gtttggaata atcgaaggat gcgtccaaat aaatccagcg taacagctat
    17461 aagatatatc aacccatcaa tcaatttgtt atcaaccaag cgcagaaata ctttttccga
    17521 tacccatttt gtaggttcca caataaattt atcatagaac tcatctatgt aatatttatt
    17581 gagcagcgtt ttgtagattg gttggtagtc tgctgcgatt ttttcagcgc tgactttctt
    17641 agccatatag atcacccacg ctataccgat acctatgaca ccggccaaaa ccgatatgac
    17701 catcagcgtc atatcattga agccgtgatg ctcaacgggc gccgcatgcg taccgaccga
    17761 cggttcaaga aaatgcgcaa gccaattatg accacctaga accggaggaa taccgatgag
    17821 accggcaatt gccgcaaaca cagctaaaat cataagcggc acggtcatag tcgaagtaga
    17881 ttcatgcaag tgttcttttg cagcttccgg agtccggctt tctccaaaaa atgtaaggaa
    17941 tactaatcgt gacatgtaaa atgccgtcat catcgcaccg agcacaccaa accagaaaac
    18001 gccattgtat cctttatgat aagcggatgc caaaatttcg tctttactaa aaaatccgga
    18061 aaaaggcggt aatccgcaga tagctaccca accgatgaga aacgtaatgg cggttatttt
    18121 agctttggaa aaaagtccgc ccatcttgcg aatatcctga ggatcttcat ggattccggc
    18181 tttgtggtat gcgtgttcca tcacatgaat gacactaccc gaaccaagga acatcagcgc
    18241 tttgaaaaaa gcgtgtgtca gaagatggaa aacaccggcc gcataagcgc cgacaccgac
    18301 accgataaac atatatccaa gctgagaaac cgtagaatac gccaaaactt ttttaatatc
    18361 cgtttgtgta aaaccgatgg tcgccgcaaa caatgcggtt gccgcgccta catacgtcac
    18421 caagtcgccc gccgacatgc ccataatgta tcccgccggg ccgatgtcaa atgaaaatat
    18481 cggactgcaa cgcgctacca tgaatacacc ggcagtaacc atcgtcgcgg cgtgaatcaa
    18541 agctgaaacc ggtgtcgggc cttccatcgc gtccggaagc caaacataaa gaggtatctg
    18601 cgccgatttg cccatcgcgc ctacaaacag aagcatacca atggccgtca tcgtcgtgcc
    18661 gagcgcataa ggatcaactt tgtgtatggc gtcaaaaagc gtttcaaaat gaatcgttcc
    18721 aaacgtagca aacaccaaaa atatacccaa tccaaagcca aaatcgccga tacggttgac
    18781 gataaacgct ttttttccgg catctccggc gcttttcttt ttataataat acccgatgag
    18841 aaggtaggag cacaaaccta cggcttccca gccgaaaaac ataacggcaa ggttatttcc
    18901 catgaccagc atgagcatgg aaaaaataaa cagattgagg tacgtaaaaa accgaggacg
    18961 atccggatcg tgttccatgt atcctaacga atagacatga atcaagaaac cgataccggt
    19021 cacaatcaaa agcattacgc cggtcaaagg atcaatcaaa aaaccaaacg tgatgcgcaa
    19081 ggtatcaaca ataatccaat ccaagtaatc aatgttgtac ttttgaccac ccaacacttg
    19141 gataaaaaca tacaacgaca ataaaaatga aataaaaacc aatccggaag caagatatcc
    19201 ggcccagggt ttggcatgat gatgcgcgtg gccttcctca tgaccttcgg tgtgatgact
    19261 tccctgccct tcgatcatgc ggccaaaaaa cacattgaat accgcgccgg ccagcgggat
    19321 caggggaatc attagaataa tcatttccat ataacaacca tctccataaa atttccgtcc
    19381 accggtcgga cgctactaac cttgcatcat atgcatttca tccacattga tcgtgtcttt
    19441 cttacgatag atcgctacga tcaatgccaa acctacagcg acttctgctg cagcaaccgt
    19501 cataaccaaa aaaaccgcga tttgtccgtc catcgaatcc aaataccgtg caaaggctac
    19561 catcgtcaga ttcacggaat tgagcataag ctcgatacac ataaatacga tgatcgcgtt
    19621 gcggcgtgtc agtacgccaa caacgccaag cgaaaataat atcgctgtta ctatcaaata
    19681 atgagagagg ggaaccatat ttgctccagc acttattgtt tacgtttgga aataataatc
    19741 gcgccgacca tacccaaaag aagaagtatc gaagcgattt cgaaaggata caaatactgt
    19801 gacatcagta ccgagccgat gacttcgacg tttccggcct cacgtatttt ttcgatcgga
    19861 taaattcccc gatcaccgag attgtacgta actttcgttg caagaccgat ttgtaccagt
    19921 atcgcaaaag ccagcatgat cgagatatac ttcataggtc cggcgtattc ggtcagatgt
    19981 gcatcacgac ccaaattgag caacatgatc acaaaaagga atagaaccat tatcgcgccg
    20041 gtatatacca atacttgaat tgcggctata aattcggctt ccagtgtaat gtataacacg
    20101 gcaatacaga agaacacccc caccataaaa atcgcactat aaacggggtt ttccatcgtc
    20161 agcatcatga tagcgcatag gacagcaatc gcagcaaaaa tataaaacac cggatttacc
    20221 atcacatatt gcaacatatt ttccatagta cctctaaact ttattctgat cctcggatga
    20281 tatacatcag ccgcagtatc ccgtcatctt tttagtgaac cgatccgtgt tcgtcagagt
    20341 gtttgacgtc tttcacggta aaataactcg aatttttcat ttgtttatgc aaaccgtcgc
    20401 gccgttgtgc acgatccggg tgacgcttga tgtattcatc atatacaccc aaaagacgtt
    20461 ccttattgta aatgaaatcc gcgcgcgtgt aatctgcaag attatgcata tccgtcatat
    20521 caatcgcatc ttcgggacaa gcttcaacgc acataccgca ataaatacaa cgcaattcgt
    20581 caatttcgaa aactttagga tgtttttcgc gattttcgtg cccgtctttc catgcatcac
    20641 cgctgtcgtt cgctacgata cgaatacaat cggcggggca tgccgtagaa cacatttcgc
    20701 atgctacgca tttttcgcga ccgttttcat cccgcgtcag catgtgcaga ccgcgataat
    20761 tcggaggaag aactttttta tcctcgggat aactgagaac atcgcgtttt ttgaagagat
    20821 gcttaaatgt aatacccaac ccttttaaga tcggtattac atacgtattg tccagccaac
    20881 tcttaccgcg gggttcagaa acatttttta cattcacagc catattgtta taaaccttgc
    20941 gttatacctt ttctatagcg ttatacgaaa gccagccaaa tagccgtaat caaaatatta
    21001 gccagcgcca aaggaatcat ttttttccaa ccgagattca tcaactgatc ataacggaag
    21061 cgcggaagcg tccagcgtac ccacacatag aaaaacatca ggaaaagcac tttaaatgca
    21121 aaaccgccaa gattgatcag atcgcgtgtg agcggagaaa accccatgtc atttacaaaa
    21181 ggaatatgcc aaccgcctaa aaagaggcat gtgatcataa tcgagtttgt aagcatattg
    21241 caatattcgc caagataaaa aagaccgaat ttcatcgaag aatactccgt gtgatatccg
    21301 gcaacaagct ctgcctccgc ttccgtcaaa tcgaacggcg tacgattggt ttccgcaaaa
    21361 gcggtcgcaa taaataacaa tgctgcaagg ggttgaaata cgatacccca catgtggttt
    21421 tgaatctgaa aattgacaat atcttcgatc ttaagcgaac cgacaaccag caccatactc
    21481 atgatcgcca tacccatcgg taattcgtag ctgatcatct gtgcggccga acgcataccg
    21541 ccgaacagcg agtatttatt attggatgcc catgcaccaa atgtaatcgc gtaaacactc
    21601 aacgaagaaa ccgccaacac ataaagtacg ccgatgttgg tttgtccgat ctggatccag
    21661 atttcacgcc cgccgacggt gataaaaccc caaggaatca cgcccacgac aaaaaacgca
    21721 ggaaacacca tcaatgcggg tgcagctaca tacaaccatt tattgacatg caaaggtatg
    21781 atatcttctt tggacatcat cttaagcgca tcggcgatcg gctgaaaaag acccagcgga
    21841 ccgacacgat tagggcctac acgatactgt acccatgcgc tgacacggcg ttcgacgatc
    21901 aacatgatcg gcactatcgc aagatagatc gccagcatca atccgatcac taagtacggt
    21961 acaaaaggta aaaacgcatc catagtcaaa tactcgttat aaatgaaaat tctataatac
    22021 catttgttcg ttcgtatatg ctaactgtgg ataaagttta tccatatcca agctgctata
    22081 cgacgaaaca gtttctttaa tctctttggt aatgtctgca acggattcgt aatacaatcc
    22141 gtattccaga gccgaagcta tatccaaaac gatctgccac actgggcggc tttcaccggc
    22201 gggttgaatg gctttattaa atttctgaac caatccgtct ttgtttgtaa acgttccgct
    22261 ggtttccgta aaaatagtcc cgggtaacac aacatcggcc tgattggtaa cggcgtcttc
    22321 gaaatggcta atcacgatca atgtttccgg cttggttttc aactctatat tatggctgcc
    22381 caaaagaact actacctttt tacctgcaat ggtttgttcc atttttgaat cgcccaaacc
    22441 aagaacttct tttgcaccac ggtagttcgg agctttttcc gattcgatca taaagggata
    22501 tttatttttg tccgtcttaa atgtttgtgt atcgccgtat aagggcttgc tgacatccaa
    22561 cgaagcgccg aatgatttgg ctaatttacc gaacacataa ttttcttcat tggaagcata
    22621 agggctcgcg atgaaagcca ccgatccgga acctgccgat ttgatttttt cagtaaccag
    22681 cgccatagcg gccgtccaat tggtttcact cagcttgtta tctttgcgtg tatggggtac
    22741 caatatacgt gtcaattttt cctgagcatt cagatgataa cggccatcat cgcaaatcca
    22801 ccattgattg acggccatat tttcgcgcgg gcgcacacgg taaactttgt ccaccagttc
    22861 ttcaagattg agtttttggc gtttcatggt atccagcgtg gtattgcaac ctttactgca
    22921 accggggcag acgctttcgg tgtgaacaaa attccacaca cgtccttcaa atttttcgtt
    22981 tttgtcgatc aaacatccga ccgggcaaag atctacgaca ttgcccatca ttttattatt
    23041 caacggcttg ccgggattta cttcgatttc agcgcgggca ccgcgatgat taacttttaa
    23101 ttcttctgtt cctgaaattt cttcggtaaa acgtacgcaa cgtgtacaca tgatacaacg
    23161 ggacgtatag agataaatcg aaggaccaag atcttttctt tgcggaatac gtttgatttc
    23221 agcaaatcgc gattcgtgat ttccatgttc atacgtataa tcctgcaatt tgcattcacc
    23281 ggctttatcg cagatcggac aatcgatcgg gtgattgagc aacaagaact ccagaacagc
    23341 ccggcgcgca gtagtgactt tttcgttttc agtaaaaatt actgcatcgt atttatcttc
    23401 gatttttttt ccgggcggta attctccgac cgtcgtagaa caacccggta caagtttcgg
    23461 agcgccgacc atatccacca aacacatacg gcaacttgct acgattttca tgccgggatg
    23521 ataacaataa taaggaatct ctatgcctat gcgtttcgcg gcgttgagga tggtttcccc
    23581 acgctgcatt tccaattcct gtccgtcaat tttgaccagc gccattatct gtttccttta
    23641 atatcgcaaa attcttcgcc gcgtttggac agatccaaac caaggcgttt acaaaaattc
    23701 attttttcaa ttggcagttg agcatccact gaataccgaa tttatcggta aacatgccaa
    23761 acttagcgcc ccaaaatgtt tcctgaagcg gcatgatgat cgtacctccc gcagataact
    23821 tactaaatac cgcttcttgt tcggccagtt ttgtaaaatt gatgctaagt gtcacattgg
    23881 aaccgcttgt tacgggttgt cccggcatcg catccgaggc cataaaatac acggcgtcag
    23941 ctttaaactc cgaatgcatc acgagatttt tttgctccgg cgtactgtcc atcggagcgt
    24001 ccccgaaagt ttgaagcgaa agtattttgc cgccgatact gtcacgataa aattccattg
    24061 cttcacggca gttgccatta aaacaaagat acggactcaa tcccttggcc gggatttttt
    24121 ccgtcggttt tttcaccgtt ttttttaagg ctttcacctt tttacgggct acttttttag
    24181 cggctttttt gacaaccttt ttcttcgctt ttgccatggc tttattcgca gttttttaat
    24241 tccactcgtg aggaatggtg gtcatacact tcttattggt aatatgatat tcaaattctt
    24301 cgcggaattt gttcacaaaa ctttggccgt tgagatcgcc gcctttattc ttatccggca
    24361 atcctgccac aggccacgcc gcggcatcgc cgagcggaca aatcgtgcgt cctgcaatat
    24421 tgttagctgt ttcaagcaat agatccaagt cttccatgcg gcctttgccg gtttctacac
    24481 ggccaagtat catttccaac caatgtgtac cttcgcggca tggtgtacat tgcccgcaag
    24541 attcgtgcgc ataaaacttg agaagattcc acaacgcatt gaccatacac gtcgtttcat
    24601 ccatgaccat acaaccggcc gaccctaaag ctgaaccgac tttagctaac gaatcaaagt
    24661 ccatattgac attacactct tcagcacgaa gaacgggcac agacgaaccg ccgggaatta
    24721 ccgctttgag tttacggcca tgacgaatac cgccgcaatg ttcgttgatc aaggtaagca
    24781 aattggttcc cattttagct tcatacgtac cgggacgagt gagatgaccg ctcatgcagt
    24841 acaattttgg ttcattatct ttccaccact gaatgccacg attgatgata tgtaccggcg
    24901 tcactaatgt ttcgacattg tttacgatcg tcggacatcc gaaagcaccg aaaacagccg
    24961 gaaatggcgg cttgatacgt ggccagccgc gtttaccttc gagggattcg ataagaccgg
    25021 tttcttcacc gcaaatataa gcgcctgcgc cacgatgaac tgtaatatcc agattaaaac
    25081 cgctaccgag tatgtttttt ccaaggtacc ctttagcgta agcctcgtct atcgctttac
    25141 gcaaagtcaa agcgcctttg acatattcgc cgcgaatata gatataacat gtgttcgctt
    25201 gtatcgcaaa agatgagatc acaagccctt cgatcacctg atgcggatcg tcttccataa
    25261 gaacgcgatc cttgaacgta cccggctcag attcgtccgc attgcaaaga agataacgcg
    25321 gtttgggatt atctttgggt aaaaaactcc atttcatacc ggtcggaaaa ccggcgcctc
    25381 cgcggccgcg gacccccgca tctttcatca tttggatgag ttcgtccggg gtcattttta
    25441 aagctttggg caaagcctga taaccgccgt gcttgatata cacatcaatg ttttgggagt
    25501 tttccacgcc caccattttt gtcagaactt tttccatgaa cgatatcctt ttacactgcc
    25561 ctaaggcagc acatcttata tactatgaag ttcgtaaacg cgctctactt tcgttggtat
    25621 gtcttattta caggcagtta tcagcgcgtc tattttttct ttcgttaggt tttcatgata
    25681 atcatcgttg agttgtaaca tcggcgccgt accacacgaa ccgaggcatt cgacttttac
    25741 gatggaaaac tttccatcgg ccgaacgtcc ttcgccgcat tttacgccgc attttttctc
    25801 caaatagtcc accacttggc tggcaccgtt gattgcgcag cctagagtat ggcatacctg
    25861 aaaatgatat ttaccgactt cgtgctggtg aaacatggta taaaacgtca ccacgtcttc
    25921 gacgcgcgat accggctcat ccagcgtctt tgccacaagg ttcatcacat catgggaaat
    25981 ccagccgaat tgttgttggg cgacatgcag caccggaaga gtcgccgctt gtttggtggg
    26041 atagcgggaa attagccatt ccacttcttt tttattttcc ggggaaaatt ctaacgtcat
    26101 aatccaaatt cgtttggtta aatccgtatc cgttttgttc aagtgcgcaa catgcgtgtg
    26161 cacactttat atcaaactga aatcttttta ccgatcacat tcgccggcga taacattcat
    26221 actgcccaaa atagccacgg catcacctat caatccgcct ttggccatgt gggacatcat
    26281 accaaagtga ataaaagaag gcgcgcgcac gtgcacttta tacggcgttc cttcgccgtt
    26341 acttacgatg taccaaccca attcgccatt gggcgcttcg gtcgcatcgt atatttcacc
    26401 cttaggcatc gcaatgccat gttgatccat gatcaacttg aagtgatgaa tcagtccttc
    26461 gatggaaccg tatactttat ttttatcagg taatgcgatg gtgtgatccg ccacttttac
    26521 cgggccgggt ttcatgcgtt ccaaacattg ttcgatgata cgaattgact gcttcatttc
    26581 ttccatacgc acgaggtagc gttcgtacac atcaccttcc gttccgatcg gcacatcaaa
    26641 atccacttca tcgtatttaa gataaggctc atcttttcgg atatccgtcg caacgccgct
    26701 ggcacgtaaa caaggtccgg tcataccgca attaatcgcg tcttcagcgg aaattttccc
    26761 gacattttgc gtacgatcca gaaaaatctt attgtgattg agcaaacctt ccacttgatc
    26821 cgtacacgtt gctttaaact gtttacacca ctgtgcgata tgacgcggat ggtcttcggg
    26881 aaaatcacgc acgacaccgc cgatacgcgt ataactggtg gtaagtcgtg aacccgtgga
    26941 ctcttcaaac agatcgtaga taaattcacg ttggcgaaac gtataaagaa atacggtaaa
    27001 cgcacctaga tcaagcgcgt gcgtaccgat attgaccaag tgatcggcga tgcgggaaag
    27061 ttcgccctgt atcatgcgaa tgtattgcgc ccgctccgtt agggtgatac cgagaagttt
    27121 ttcggcagct tgcgcaaaac cgatattatt ggacatcggc gagaggtaat tcatacgatc
    27181 cgtgaccgca ataaactgat tgaacgtatg atgctctgcc agtttttcaa aaccggtgtg
    27241 caaataaccg gggcttggat ccagattgac gatggtttcg ccgtccaact gcatgaccat
    27301 acgcagtgtg ccgtgcgtag ccggatgctg cgggccgaaa ttgagcgtaa gaagctgtgc
    27361 gccgggaaga gataatgatt cgtcatcatc accgatctta acgtgttttt tatacgcttc
    27421 tttagctttt tttagagttt cttctgagga ttgatcttta aaatcctcaa ccatttctga
    27481 acgatctatt tccatactta cctttccaac gcctctatgg aaaaaatttc aaaataaatt
    27541 caaaaaaact agctgccttc gggcaagatc acatcgcgct cgcctttacc gcgtaagggg
    27601 tagtctttgc gcaaaggaaa acttgtaaac gaagcaggtg tcaaaagacg acgaaggtcg
    27661 gggtgccctt cgaaagtgac gccgtacatt tcatacgctt cacgttcagc ccaatcggct
    27721 aatttccaaa gatgagatac cgtggcgatt ttgcaatcgt tttccggcac cggcgctttg
    27781 atgcgaagac ggcgattgta tttggtcgaa cgtaaatgat aaacaacctg aaagcgtgga
    27841 tttttttcca tgatcaatcc gtccacgacg gtcacatcat tgagcatatc gtacgccaaa
    27901 tcggaatcgt ttttaagaaa tgccatgatt tcagctatgg attcgcgttt gatgtacaga
    27961 gtgagttcat tacgaaacac ttccgatttg agcaatgatt cgccgaattt ggatttaacc
    28021 gcatccagtg tcggattgtt ttccaaaata tttttttcgt tgttaggcat aatgtttctc
    28081 agtgcgattg atgttatcta gttcaggggt tttattccca ctccagtgcg ccttttttcc
    28141 acgcatacgc atacccaacg agcagaatac cgataaagac caatccttcg atgagaataa
    28201 aagtacttac cgacacccac ttgttgtaaa tcacggccca cggatacata aagacaattt
    28261 ctatatcaaa aaggataaaa agcatggcga taagataaaa cttcactgaa aacttagggt
    28321 gggcatcccc gacaggcggc ataccggatt cataaaccga aagttttcgt tcattaggct
    28381 tgtagggacc gagaagccgg gatagtatca atatggcaat cgggataata atagccaatg
    28441 ccataagtac aagaaccgga agataattga gatagacggg cccgtcctgg aggatgaaga
    28501 gcatgggaaa cctcgtgctg aaaaagctct atttttaaat caaatttttg gaaagcaaca
    28561 ttttaaaaag cggacaattt taagaatttt gctaataaag tgcaacagat atttcccaat
    28621 acgtaagtag ataaaaataa aaagggttac ccgaaaagac cttttgtgtc ataacgggta
    28681 accctatctt ctctatatag cttctctatt atataggtta gatctactaa cttactttat
    28741 agacccagtg atttcaaaaa agcttcctga ttcggttcac gccccaaaaa ttcttttacc
    28801 agcgcatacg gatcctccga accgccgcgc gccagaataa tatctctgta acgcaagcct
    28861 gttttttggt ccatcacacc gtttttttca aaaacggaaa acatatcagc ggcatatact
    28921 tcagaccaaa gatatccgta ataaccggct ccgtatccat tgagatggcc aaatgaagct
    28981 tgaaaattgg tcccttcgag gtaaggaaaa agagtgattt cgttttgcaa acgcttcacg
    29041 atatccgtag acgtttcggc gcctttggga ttccatttct catgcaacgt aaaatctacc
    29101 ataccataaa aaacctgttg taaagcgccg atgccggaac cgacattttt ggcggccacc
    29161 attttatcga acatcgcttt gggcaatact tcgccggttt tgtaatgctt ggcgaacatt
    29221 tttaccacat cataatccca tgcccagttt tcaaaaatct gtgaaggcgc ttcgacaaag
    29281 tcgcgcggta cggccgtacc ggattgtgaa tacaattccg atgttgtcaa tatgttgtgc
    29341 aagacatgcc cgaactcatg aaaaaacgtc tcaaccgaag cgctacctgc cgagtgatac
    29401 atcagcgaag ggcgatcccc gctcggtgcg ggaaaattac attccaaggt cgccgcaggg
    29461 agttggtaac cattgggggt tgatttacct ttaaccatgc caaagcatgc ggcgtgaccg
    29521 tatttattgg gtctcggaaa aagatcaaaa taaaaatgcg ctattacttt tccttttttc
    29581 cgcacctcaa acatgcgcac atcgctgtgc caaaccgacg ggttttttat ctctgaaaac
    29641 tccacatcaa aaagtttttg cgatatttta aacaacccct cggtaacatt gttcagttca
    29701 aaatattctt tgatcttttc atcatcaacg ctgtatttct cacgcatcag aataccgttg
    29761 taaaaactcg attcccaggg attgataaca gacgcggaag catctttgag gtaattgcgc
    29821 tttacagcta ataattcatc gtagtcacgg cgcgcctttt ctttaacttt atcaatcaat
    29881 gcgctttcaa aattccaaac attttccggt ttttttgcca tacgatcttc gacacgatat
    29941 tgtccgtacg ttttataacc cagtagcgta gccatctctt gccgctgacc aaccaaatca
    30001 cgaagcacgt gaagattttt atcaaccgag cggttattat acttgacata caatgcttta
    30061 cgtgcggatt cggattgact atatttcata aaaggcacat acgacggata agtcatatct
    30121 atcctat
//
LOCUS       DAOQGF010000035        28607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_740_length_28607_cov_6.519228, whole genome shotgun sequence.
ACCESSION   DAOQGF010000035 DAOQGF010000000
VERSION     DAOQGF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28607
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_740_length_28607_cov_6.519228"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK937_06865"
     CDS             complement(<1..446)
                     /locus_tag="PK937_06865"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282944.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNH32328.1"
                     /translation="MPRIFSGMRPTGKLHLGHLVGALENWVKFQDAYQTVYGIVDWHA
                     LTTGYKDTASLRNYILDTAIDWISAGIDPQKSIIMQQSRVKEHSELHLLFSMITPTPW
                     LIRNPAVKEQARDMGLIETTQDEQMMRIDFGHLGYPVLQTADILIY"
     gene            complement(531..1040)
                     /locus_tag="PK937_06870"
     CDS             complement(531..1040)
                     /locus_tag="PK937_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNH32329.1"
                     /translation="MALNGHVLVLNQNYEPMTICHVKKAFVLVFLGKAEIIEIAEGRS
                     IRSVSHEYPFPSIVRLMSFVYKKRKGIMLSRKNILKRDNYQCQYCGTRSDAMTVDHIL
                     PKVRGGKDAWENLVTACITCNNKKGDRTPDEARMVLMTTPRRPHPLSFIQGHASGHYD
                     NWKQYLFMS"
     gene            complement(1132..1583)
                     /gene="rnpB"
                     /locus_tag="PK937_06875"
     ncRNA           complement(1132..1583)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK937_06875"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1785..3911
                     /locus_tag="PK937_06880"
     CDS             1785..3911
                     /locus_tag="PK937_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNH32330.1"
                     /translation="MLRFFNFCIMMFSFVFLISLASCSSAKETTSSPTSSVVSVRDSL
                     TMKDTVKPETNEQPVQFLELTPAVSGLSNGSVEDTTAIASVKANYSLALKAWYVDQNA
                     SLAKSHYQKAVDALVAWGDVLAKQETTDDNANNLFDPNIDLSEAAPADDTKTENEENG
                     VNGKMDYPNWLASGIVDQYKNLLEIQNEYKEDSFQQTVISRLVFYDIGLSGSNSIGTI
                     KAPIAKGHAMDFIPINIDNERIRKFTDYLTKNGGANIKYLYRKFGRDADLIRTIIREE
                     GVPDDMIYLAMIESGYSAQARSHAHAVGHWQFVKKTATGYGMNVDWWVDDRRDLYVST
                     RYAFKYLKDLFNEFGDWYLALAAYNAGSGRIYNAMKKNNTRDYWEMTKLPRETRNYVP
                     YFIAATQIVRDPAKYGIALETESAARMDTVTVTECLDLEIIAQCVNSTPDSIRDLNPA
                     LLRWCTPPTVPNYLLKIPFGTRTAFLENYAKIPQDKKRSWVRYQVKYGETLSGIANRY
                     GTDVKAIQQANQLKSSVNLTTGQWILIPVAPKSYVATKEPTPVRNTPVINASNQAKTT
                     NAGGTGKTNTNWMVGKKKLTFKVEAGQTLGHIAEWYNITAQNIRDWNGLYYGDPIFPG
                     QVLTLYIDQYMPDEGYRNATNKRTKEVTAEEPGMKIYVVQDNDNLISIAKMFNVEVSN
                     IKRWNKLSSNTIKVGDKLKIYLNDKQ"
     gene            complement(3970..4557)
                     /locus_tag="PK937_06885"
     CDS             complement(3970..4557)
                     /locus_tag="PK937_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32331.1"
                     /translation="MKRIIYSLLVMLAAVSCDVDVEDTSPNSYAEFMSEGWILFSQTY
                     YDSSYHTFADAIKKYPDSADVYTGAGWALMKMDSLNKAGQTFITGSTKVGTDVDFYAG
                     WSFCENARRQFSSSNEKLMTALSLKPTWSFPYAGNIGASDLKAMKAENYFMMGDFGSS
                     LAAVQVLDPNFTADIQTDTGIGELAVKIEALKKAN"
     gene            complement(4569..7400)
                     /locus_tag="PK937_06890"
     CDS             complement(4569..7400)
                     /locus_tag="PK937_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNH32332.1"
                     /translation="MQKLYVAGLVFLLSYSSGVIIAQTNMTFLSQKDDHGSNYANLWG
                     YVDSLGNEYAIVGCFNGTAIINVTNPTSPTEIAYIPAPSSSWHEVQVWDKYAYVVSEG
                     GNGLQVIDLSQLPTSASLVQNWTTPNYFRTHALQVRNGYAYLTGGNVTQGVPGGKDIG
                     GIKIVSLADPRNPVEVGNWAENYVHDCYVRNDTIYAASIYQGIIYVIDTKVKSKPRTI
                     HQFTYPNAFSHNTALSDDGNYLFTTDETSSPAGRLRIWNISTLRDGIPNNTNISQVKD
                     FGTTAIVHNVYVKGRYAYASYYTEGVRIWDLNNISTLPVAGAYDTYDADNSANFTGAW
                     GVFPFFPSGNIAISDIVRGLIMISFSGKETGQISGTVKDVNTDLPLTGVKVALLEDTQ
                     TRLTNALGQYNFSALHGATTVKLRKPGYRTAYHYTTVPLNATSTYNFSLTPLTAKSPT
                     SISAFADSALVDLVWNSPDPNDIVKYRIYYGMSPNPTTQMDSTIDSRDTTRSISTLTV
                     GGTYYFRVKAVYWDGQVSDFSTETSALIPNKKPVATTILNILSDSAQATIRWRKSNDS
                     DFLRYRIFGGTSPNPTTQIDSISAQNDTVKTITNLMNGQTYYFAVSVVDVDGLMSRLS
                     NEVSVLVKPIVFNLVTALYQNSVLPQYINVVVNTNIALVSPPSVRFWKTNDTTSVSLS
                     VLPGTANTYSGSYQLKQSGTYTIQTRGISFRNQDSTKLRYLTATLAKPSSDNRLTTED
                     GASVLMIPGSAVTTDMFFTCSIEKNEETERIYAFGPDINYTVPLQLQFKLPALTNEQS
                     KKWFILREENGQWVKIESHVLANENIIRTNLSSLGRFKLQYDPTSGDETLIVKSFDLK
                     QNFPNPFNPETTIAFTLENNSDRLTLTVYNTLGQKIKTLWDGPKTSGYHRVTWDGTNE
                     TGQRVSTGTYIYRVNTGKSVFTKKMLLIK"
     gene            complement(7436..8683)
                     /locus_tag="PK937_06895"
     CDS             complement(7436..8683)
                     /locus_tag="PK937_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HNH32333.1"
                     /translation="MIILKKIEHYKGELSLGIMVTGLLSCFFIAASYPHFLPMLVFIL
                     QSGFEAGTVGGAADWLAVKMIFDEIKIGRFRIVPASGIIPRKQKSIADGAGKLVAEEW
                     LSKDSITGWLSSFDAASALAVQLKKIEQNGELDRFINWARARIIQWLDSPESNSYFEM
                     LLREGLKSIKPIHFLGHSVPDEQFTKILHRLLDHAPEKIADTMTSQTVYELLRDNIER
                     EQGFFSKLFFDAGELTERIITKATSILRSIAADENHELRLEIIKRSMAWKQTLKNQDN
                     NTWDTWIQEIISETDLKQYIPAIKVKLKSLLDSETDSGKKIDELLKNIWHNTIFMLES
                     DRSLQIAVNTRFIEIASDMLQAHHGKIAELVSYNINKLSVQEIRDQFRSRTYDDMQWI
                     RVNGAVAGFAIGILIGLFRLIIG"
     gene            complement(8689..9315)
                     /gene="tmk"
                     /locus_tag="PK937_06900"
     CDS             complement(8689..9315)
                     /gene="tmk"
                     /locus_tag="PK937_06900"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNH32334.1"
                     /translation="MNRGIFISFEGIDGCGKSTQIRRLQMRIEAEGLPVIAMRDPGGT
                     TVSEKIRQILLSTDSVIDPVTELLLYEAARAQILAEKIRPSLAEGYVVLIDRFFDSTT
                     AYQGYGRKIDLALIHAANHIATQGLSPDRTYLIDISWDESQKRRIASQKDRMENEGRD
                     FFECVRQGYLALAEKESGRVFRLDGDQNIDRLSNLIYQDFLTLRDRTK"
     gene            complement(9312..10061)
                     /locus_tag="PK937_06905"
     CDS             complement(9312..10061)
                     /locus_tag="PK937_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNH32335.1"
                     /translation="MIDEALIRRLQQAKRWVFFTGAGMSAESGVPTFRDAQSGLWAHF
                     KPEELATPQAFKRNPKMVWEWYAMRRQMIAAAQPNAGHYVISELEKHFEVTTITQNID
                     NLHTVAGSTRVLELHGNIFRTKCFTDDKIVEHPLDTDDIPPLCPRCCGYVRPDVVWFG
                     ELLPEKIFEQSMHAASNTDVFLCIGTSGVVYPAAGLPIHARQSGALLIDINPETTPIS
                     EQAAYHIQGTSGPILTQLFEKVTGHFMESRT"
     gene            complement(10061..11521)
                     /locus_tag="PK937_06910"
     CDS             complement(10061..11521)
                     /locus_tag="PK937_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNH32336.1"
                     /translation="MSDHVLKDLEPKRVFDYFYEITQVPRPSKKEEKIRAYIKGVAQK
                     LNSDWSEDAAGNIVIRKPAAPGYENKPVVVLQGHLDMVCQKTPDTQIDFDNDPIKPRI
                     DGEWVKATGTTLGADNGIAVATMLAILEDTTMKHGPLELLFTVDEETGLTGALKLEPS
                     MLKGRYLLNLDSEDEGVLFIGCSGGKDGVIHFPIKRKPAKENETRCLIAVEGGVGGHS
                     GMDIILGRANANKILTRVLYELTKEYKFKLSDIKGGTVRNAIPRDAAVHIAVRKEDIG
                     AFKVRVQKMHNYLSAKYQSTDPDLKIVFKEAPIAQFSTIKRSRSVELIRLLKSLPNGV
                     QAMSKDVDGLVETSVNLATIQTHEKEIIIGSSHRSAIEEEKRDILDQVGAICSLTHAT
                     VKETDGYPGWKPNPQSKLLAITKKVFKEIYGKNPKVTAIHAGLECGIIGEKFTDMDMI
                     SFGPTLRNVHSPDEGLHIPTVKPFYTAVQKLLETIA"
     gene            11651..12313
                     /locus_tag="PK937_06915"
     CDS             11651..12313
                     /locus_tag="PK937_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32337.1"
                     /translation="MKLKKILIVDNDPASLVNLSAMVEKAGFTPVGFQKPTLAMEFIE
                     KPDNHDIRMIIAEMNIPGSDGFTFLKAIQKKPQLKLMPFLFLSELQDTAVLVNAYEQG
                     AVDYFIKPIRKESLFIAKIKSMVDAFERTLQSANTLLSGQLSDKPLVELLAVCENESL
                     NGYLRIKHADGIGVITFVKGLPEKMAIVDAQQKIIKSESEAFEAMSDWSEGEFLIRRG
                     NL"
     gene            complement(12324..13298)
                     /gene="meaB"
                     /locus_tag="PK937_06920"
     CDS             complement(12324..13298)
                     /gene="meaB"
                     /locus_tag="PK937_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNH32338.1"
                     /translation="MEHIDAVRLLAHNDPKRKRYLSRLLSSVENDAENGPDILRALLP
                     YTGQAYRIGITGPPGAGKSTLVNAAALGLAQQGKRVGIIAVDPTSAFTGGAILGDRFR
                     MQAISTHENIFIRSMATRGAMGGLVHTAHAAADLLDAFGYDVIIFETVGVGQSEIDIV
                     QYTDTVVVVLVPESGDGVQAMKAGIMEIADLFVVNKSDRDQADILKRELEAAVQIRYT
                     HAPWMPVVLKTVATQNEGIDAVLETVFKHREYLESSGDMDIRRKSRIRNALLRGVNKK
                     LHDHLTSEGYRGLVDEQIDAVFYKKIILQDAADMIFRSMYSSAIPPGK"
     gene            complement(13314..14171)
                     /locus_tag="PK937_06925"
     CDS             complement(13314..14171)
                     /locus_tag="PK937_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH32339.1"
                     /translation="MQTPHIFVIILSYNGRHLTEACVRSCLDSDFTSFTIVLVDNASP
                     DGSGDYLKEKFNSEIHSGKIKYIGNEANLGFAGGNNVGIQYALNNNADAVLLLNNDTV
                     MDKKCLVHLSEGLAHYPQAGILGPKIYYFEPNDQIWFAGAEFKLYKGTARHIGIREKE
                     NLQYEEAKTSDYITGCAMLIRCDVFKSVPGLDTSYHMYSEDVDFCFRAKQAGFELVYI
                     PQAKIWHKISASTGGQLSLRKIRLRFFSNMKLLIKYARWYHWLTIPFYFFADLMRIAF
                     RILTGAIRN"
     gene            complement(14184..15386)
                     /locus_tag="PK937_06930"
     CDS             complement(14184..15386)
                     /locus_tag="PK937_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32340.1"
                     /translation="MSSDEYKLTTLASSALFKNIPEDALRRLLPHFTLKVYPTNTVIF
                     EDGNTQPDGMYIIAKGFVKIFKSVPGEEVRQQAVAVLPAGSYFGEMALLDEEVRSAGA
                     VTMYETELLFFPKESFHALMHEDIKLSHEIMTRLAKVMSKRLRDTNNLFREVVSWGYR
                     ARKEVRDLKSNFLSTISHELRTPIHSIQGFSTLMKESKDVDDETKQRFVEVILHESKR
                     LADLINDLISLAEIEYGSIIIERHPNNLKELLSKAFDEYKDIAREKNINLEMKVEDTF
                     PIVAMDGPRFIQAIGHLIENAIKFTGFGGKVGMHALMTTDAIEIHVHDTGSGIPSKYV
                     ERIFDKFYQVDQTTTRSAEGAGIGLSLARTIVELHGGKIIVTSVEGKGSTFTVTVPLS
                     HIAFQDGR"
     gene            complement(15455..16225)
                     /locus_tag="PK937_06935"
     CDS             complement(15455..16225)
                     /locus_tag="PK937_06935"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNH32341.1"
                     /translation="MSALVIGKYSFNSRLIVGTGKYKDYDETKKALEESGAEMITVAL
                     RRVDLTKPDDKNLLDYIGSKYTLLPNTAGCYTAKDAVLTCKLAREALGATLVKLEVLG
                     DPRTLFPDNEQLLEAAQILVKDGFTVLPYCIDDPIVCKKLEDIGCAAVMPLAAPIGSG
                     LGIRNPYNLAIIIEHSKVPVIVDAGVGTASDAAIAMELGCDGILMNTAIAGAKDPIKM
                     ARAMRLAVEGGRLAYEAGRIPKKLYATASSPIEGVIKT"
     gene            complement(16318..16521)
                     /gene="thiS"
                     /locus_tag="PK937_06940"
     CDS             complement(16318..16521)
                     /gene="thiS"
                     /locus_tag="PK937_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNH32342.1"
                     /translation="MKIKINGTVYELDSVKTIDDVLQHFGVSYAHGVAVAVNYDVISK
                     DRIQTQTVCEGDEIEIIRATQGG"
     gene            complement(16524..17024)
                     /gene="def"
                     /locus_tag="PK937_06945"
     CDS             complement(16524..17024)
                     /gene="def"
                     /locus_tag="PK937_06945"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH32343.1"
                     /translation="MKVVLYGDPILRSVCKPVVAITDTLRMTVANMIETIGEHQGVGL
                     AAPQVGMSERLVVIDPRLMDRTEEVIVMINPEIRESEGSNVFREGCLSLPGVEAEIRR
                     PAAVTVSYTDMDNVSQTKRFIGVKARIVQHEIDHLNGVLFTDYLNPVWRWHLLRKVKR
                     RINGKA"
     gene            complement(17036..17392)
                     /gene="yajC"
                     /locus_tag="PK937_06950"
     CDS             complement(17036..17392)
                     /gene="yajC"
                     /locus_tag="PK937_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNH32344.1"
                     /translation="MNPIMMMWLMGGNPAPGGAAPNPLLSFLPFVFIIAIMYFMMIRP
                     QQKRQKEHQAMVSALAKGDKVITVGGIHGVVQGTKETTVLVKIADNVKVEIERSAVTT
                     VVKGSGEASAEAKSAE"
     gene            complement(17425..18237)
                     /locus_tag="PK937_06955"
     CDS             complement(17425..18237)
                     /locus_tag="PK937_06955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH32345.1"
                     /translation="MMIEIPRQARSVAIGAALQAARIITDYYGRVFTIDAKLHNDFVT
                     EVDRKSEEVIIQHILRAFPDHAILAEETGKHRNESHFRWIIDPLDGTTNFIHGMPMFA
                     VSIALEVHGQLAVGVVYEPLRKEMFVAEHGKGAFLNDRKIRVSATTEHSRCLLATGFP
                     FKTFDFIDDYLNIFKTFMTQTAGIRRPGSAALDLCYLACGRFDGFWELNLNPWDIAAG
                     TLIIQEAGGRITGFDGQNNFLYKGHVIGSNGILHDWMLSNVQKSLGEKISLL"
     gene            complement(18237..22034)
                     /locus_tag="PK937_06960"
     CDS             complement(18237..22034)
                     /locus_tag="PK937_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32346.1"
                     /translation="MNIRNSSILLTIILSLILSTPAHTQVSSADTDFLYARKLYDDKL
                     YALAAQEFGKFIRNYPSDMRIPDARYYSGMAQFNEKNYEQARRDFQFLAIDYPKDKRA
                     PDAWAKIAECYAAMGDYPSAANALTSIATFYPTAPNAVTSIIEASEYFIKAGDLRSAK
                     DRLQKLIADRPDIPEANVSRFKLAMLLKNENDWPAASGALQTVIEKSKDQELVASAMF
                     EQAGIADRLGKSDEARNAYVRIVTRYSKTKINVYALFELGNILFRERNFIDARKNFEI
                     VISNILAPEPLKHAAQINLGDILFVNEKYTLALDLYKRVASEKNDSLNALEANFKSGL
                     ASEKLNLPVAAGDYYYTITEQYASVFQSSQFVKWATLKLAQISFQLKRYKEAGRYYED
                     YITKYPKSADLDRVLYDRGILLKDRLADYAEASRMFTRLISEFPNSEKIDDAYLALAQ
                     SLRMDNQISEALDALEKLKNNYPGTLLWNDALDERRRIEYFHQYGNNTTTQSIIYLIG
                     NLIEDKPKDEITFAYARLFFEQLKDYEGAAGLFKKTILSTKNRALTEEALFYLAQSYD
                     RISEMKPTQAGYADSAVGVYKQLAVGKYADVATVRVIESTLKTISNYTDRQRKAKEMY
                     AGLLERFPGSTLKPNMLKGLGDAMMALGELKPVIVPQVPSVTKSKDKSPVTAKPDSLK
                     PLSSAMQCYEAIIKDFPDNPVAEDAFYSMAEAYWFSKQPDLYYQSLIRYAAQYPRGRY
                     IVKTKMLMARYKEEKSDYALALNNLNEIITHYFYTPYADSAAQSVGHNYLLSGQYTQA
                     IKAYLNARTQGEFALEQMDIIAAQQKIHHPLDYRLAYCYDKSGNFTKAVEYYEAYLFP
                     DNKGEYALHALTALAQMYEKKQDFQIALPYYQRIAQLYPETEAGFKALARASEMYFNS
                     EQYDSAKVAFTKLALYTKDKMQQIVFESRTIVCAYRLGQIVETGKLEKDFNKKYESDK
                     NLKLLLLNYNAEFLFELGRYYQYNKTRADYAQAVKTYTRVIEDYKTAAIMPEVLFEMG
                     VIKFNEGKSKEGFEYFQQIPQKWPDSDILPRVHLRTAFEAFRLENVQLAVEASKLALQ
                     NPRIKLADAKLGTNFLIKVYKAAGYYENGLMLMQQYLERFPDDDPATIWAMRIDIGAM
                     HKNLKSFDRALEYFKDLIKTASGEDEAEIQFNIAETYWAMNNFEQALLEYLRIPYLTL
                     GKKFDWSSAAKSQAAECYVKLSKYDEAIRMYNDIIRVNGPQSEYSAYAKQRIEQIQGL
                     KKN"
     gene            complement(22057..24339)
                     /locus_tag="PK937_06965"
     CDS             complement(22057..24339)
                     /locus_tag="PK937_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH32347.1"
                     /translation="MSGDGLTTNLNDAQKIAVQTTDGPVLILAGAGSGKTSVLTRRIA
                     YLIAEKQISPFNILAVTFTNKAAGEMKHRIERLVGYHSQKIWIGTFHSIFGKILRNEA
                     EKIGYSKNYTIYDSDDQLNLVKISMEYLGISQELYSPNIIRAKINKAKNTFQNPSQFH
                     KSVDNPMDEKAALVYTDYEKRLRQASAFDFDDLIVKPIELFQQFPETLTFYQEKFHYI
                     HVDEYQDTNRAQYILMKMLAAKYRNLCVVGDDDQSIYGWRHADIGNILNFEKDYPEAS
                     VYKLEQNYRSTANILQVANEVVRNNEIRKDKSLWTDKAQGKLITVFECDDDRAEAKNI
                     AQVIQKKIQQEKIRFADFAVLYRTNAQSRVLEDALRQMSIAYVIVGGVRFYDRKEVKD
                     VMAYLKLISNPADVISLQRVINFPARGIGKTTLEKLEEFSQVKNMPLFDAFRNVRSFG
                     AGLIQERTMAAIVSFCEFIQKYQELRTQMGFGEWVRILIDELGLLKMYQQENSVEAIS
                     RYENIVELLNAVSEFSMKYDAEQAEEPVLEAFLKEVALVADVDKYDENKNAVTLMTIH
                     SAKGLEFPLVVVAGLEEGLFPLENARNNARELEEERRLFYVSTTRAQNELYLSFAKRR
                     MRFNNIYQTDRSRFIEEVPAKLLDWQKISTYEPRLFEGSGKSWQSKNYSGSERVTAYE
                     DGPSRVKKSLKDFSDEIVTSAAITETKYRIGQQVIHASFGTGTIKNSEGQGDKQKLTI
                     LFGDGVERRLMVKYANLMVK"
     gene            complement(24460..28563)
                     /locus_tag="PK937_06970"
     CDS             complement(24460..28563)
                     /locus_tag="PK937_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH32348.1"
                     /translation="MKDTLVTFVVIDSSDGGQLTSTIPVLTDINGQAIAVYQTGTNVG
                     YNRVAAISYIPGSVIGMPDSTYFELITIPLDANAQPVASSLSPTIVSRNQNVSFKYSF
                     VNTGTFPVNLIGDSTYIQITHTGSGSSYRTTLDTNVNKTIGITGATQLTFRADTVKLP
                     SGVYGTGGDSMRIVLYGTLFDSAAATLDTLKFTFFTDQLDPLTVQNPAFLVIDSVLID
                     SALAVQGQDSILVRYYVRNTGEADAISFTVTDSISNAGATVTNDWVLLATNKPSTISG
                     LSSQNFTQYYRVKGNAVLGTDRISSMVRAVDYNDAAQIKYDTLYTSDTLGIRVPAQLQ
                     ITRTYIDTVYNTGILNRDQIVRIKSLVRNFGEESANLRVHFESDAGNADLDSMLTTTL
                     NGGDSLWAVSRYFRTRNASGAESYRAFVDSGYGLISGRPLDIVDPISGNQVTFTLQDS
                     AELKLNLFVDIPGDSLEVSDTANFNLRVTVTNIGEASLNTALVPVRVRLPINNMFRYQ
                     ASPTLDSVYFVTVGSTFTIPMSSYQETPDYDSVVALLDTANAAFPLDVNTNSKSAIHT
                     VRDSVFLKTSNTGNLIATRIRILSPNGAVDGTVSTFQGFTVETVVKDLSRLSGMQARM
                     ILPSEGLSTRDSLAQAFIQNVNDSVVTTWRIFSNTDNDSVYPLRVVYSGIDSTTGDTV
                     ISDTLKLSVTVVRRAEMTTTLRITGPVGALDDTLSFGQELSVEATIRNLGQAAFSGAG
                     QITLNVPVGFDVVAAPNVYTFTRDTMINWTLRANASGSLQMTAVVNVLPNDENTDQPV
                     VVFSNADTQNVVVVNPAEILNASITIQGVPGDSVSTDQIFVVSAQFTQSRSLTARYAT
                     IQFPNNSAYRLKDGEVVLKQISDLDNIVRWSVVAPSTLIIPTDNEQIDLARITFVGVD
                     RNNKDSNVTAQVDKNFTLQRKAVLRLRAAIIEPVDAIDNFVTKNQTFRIQATVSNLGY
                     ALPRGQGMLLLDSLDAGNGLIFTEQDVANRDNIFDFDNGDFTASWLVRAPDGVVNHPL
                     VLRFVHLPTDTNTRDTVTVESRSFRLAVSTEAKQLRVERIATDPGTTIYVNGVVDSIM
                     ALRLKNNSSTTNTNGMIVQEFKFVVETFDYKTRTYNDTNWTAYFDSITVRLMGEEVSF
                     GRANNLGTDEDTAVVRVDQNAGLKKLARALAVDLDYGLAILPGVAEEVVFKARVKPAV
                     AGKNPNFRLILVGVTAYDNDVLGSQDAGITDLPLLPAVDATGNLLGSPEANAGNTTQQ
                     MGIQDPENGGKVAQFGNFPNPFGSSGKEKTTFRFRGTGEKAVIEIYTLAGNLVTKLTQ
                     NTVDDTQVEMVWDGRNGNGEKVRNGVYIAVLKSGGEGKYTTKVMVVR"
BASE COUNT         7794 a         6574 c         5999 g         8240 t
ORIGIN      
        1 tgatatatca atatatccgc cgtttgcaaa acaggatacc cgagatggcc gaaatcaatg
       61 cgcatcattt gttcgtcttg cgtcgtttcg ataagtccca tatcacgcgc ttgttctttg
      121 acagcgggat tgcggatcaa ccaaggcgtt ggtgtgatca tcgaaaaaag caaatgcagc
      181 tccgaatgtt ctttgacgcg ggactgttgc atgataatac ttttttgcgg atcgataccg
      241 gcggaaatcc aatcaatagc cgtatcgagt atgtagttcc gaagagaagc ggtgtctttg
      301 tagccggtcg tcagcgcatg ccaatcaaca ataccgtaaa cggtttgata cgcgtcctga
      361 aatttgaccc aattttccaa tgctccgacc aaatgtccca gatgcaattt ccctgtcggg
      421 cgcattccgc tgaaaattct cggcatacaa cctttcgtcc gtttgagaga tcggctttcg
      481 aagccggtta cactgtcgcg gcgcctgtgt agtgtgtgat aacaagcgca tcatgacata
      541 aaaagatatt gtttccagtt atcatagtgg ccgctcgcat gcccttggat aaaactgagc
      601 gggtgcggcc tgcggggtgt tgtcatcagc accatccgcg cttcgtccgg ggtgcgatcc
      661 cctttcttat tattgcacgt gatacatgcg gtgactaagt tttcccacgc atctttgccg
      721 ccgcgcactt tcggtaaaat atgatctacc gtcatcgcgt cgctacgtgt cccgcagtat
      781 tggcactggt aattatcccg tttcaaaata ttttttcggg acaacattat gcctttacgc
      841 tttttgtaaa cgaaactcat cagtcgtacg atgctcggga acgggtactc gtgcgaaacc
      901 gaacgaatac ttcgcccttc ggcaatttcg atgatctccg ctttgcccaa aaaaaccaga
      961 acaaacgcct ttttcacatg acatatcgtc atcggctcat aattctgatt gagcaccaaa
     1021 acgtgtccat taagcgccat accttcacca cactcacttt cgaagccggt ttaatacatg
     1081 accttaacaa ccgcgcaaga taaagcaaaa actctattca atcaaataaa aattgaaaag
     1141 cagatcgtaa gccgaattct gttcgtacgc cgaagcatac ggatgatcat ttatcttttc
     1201 cggcaagccg gaaatcccgt tcactgtatc atgaccggca tgataccatg cgacggaatg
     1261 cgatctaccc gaagaagctc ttgcgaatca ggcaggcggc ccgccccttg cgggacttct
     1321 tcttatttga tcttgctccc ggaagggttt agcatggcgc cgcttacgca tacaccacgt
     1381 gcgctcttac cgcacggttt cacccttacc acaccatcgg cgtggcggtt tattttctgt
     1441 tccactatcc gttgatcacc gctttcgcga tcatcacccg accttgcatt tcttggaaat
     1501 ctcggccggt tccgcgcctt aaggagttcg gactttcctt tccgttatct ctatgatacg
     1561 gaacgatcat cgcatctgct tttcatgctt tcaaagaact ttttgagacg aatcaatata
     1621 tgatattttg atgcattaac aatcgctttg ttaagcattg ttaaaatatc tcaccttgat
     1681 aagtctttca aaaacaaagc ggcgtccatt aaactttggt tgacaatact tatcctatgc
     1741 tttatattgc cgcggtttaa aaatgtattt ctaaggagtt tgtaatgttg cgctttttta
     1801 atttttgtat tatgatgttt agctttgttt ttttgatatc gcttgcctcc tgttcctctg
     1861 ccaaagaaac aacttcgtcc cctacgagtt ctgtcgtttc ggttcgcgat agtttgacga
     1921 tgaaagatac tgtcaaacct gagaccaatg aacaaccggt tcagttttta gaattgactc
     1981 cggcagtttc agggcttagt aacggttccg tcgaagatac tacagctata gcatccgtta
     2041 aggctaatta tagtttggcg ctcaaagcct ggtatgtgga tcaaaacgca tcgcttgcca
     2101 aatcgcatta tcaaaaagcc gttgatgcgt tagtcgcctg gggcgatgta ttggccaaac
     2161 aagaaacaac cgatgacaat gctaataatc ttttcgatcc caatattgac ttatctgaag
     2221 ccgctcctgc ggatgacact aaaactgaaa atgaagaaaa tggcgttaac gggaaaatgg
     2281 attaccccaa ttggttagca tcaggcatcg tcgatcaata caaaaacctg cttgaaatcc
     2341 aaaatgaata caaagaggat tcgttccaac aaaccgtgat aagccggttg gtattttacg
     2401 atatcggttt gagcggaagt aattccatcg gtacgatcaa agcaccgatc gccaagggtc
     2461 acgccatgga ctttattccg atcaatattg ataacgagcg gattcgtaag tttacggatt
     2521 atctcaccaa aaatggcggc gcaaacatca aatatctgta tcgcaaattt ggtcgtgatg
     2581 ccgacttgat tcgtacgatc attcgtgaag aaggcgttcc cgacgatatg atataccttg
     2641 cgatgatcga aagcggatat agcgcacagg cgcgttcgca cgcgcacgct gtcgggcatt
     2701 ggcagtttgt caaaaaaact gctaccggtt atgggatgaa tgttgactgg tgggttgacg
     2761 atcgtcggga cttatacgtt tccacgcgtt atgctttcaa atacctcaag gatttgttca
     2821 atgaatttgg cgactggtat ttggcattag ccgcttataa cgccggatcg ggtcgtatat
     2881 ataatgcgat gaagaaaaat aatacgcgcg attattggga aatgaccaaa ttgcctaggg
     2941 aaacacgtaa ctatgttccc tattttatag ctgccacaca gatcgtccgc gacccagcta
     3001 aatacggtat tgccttggaa accgaatcgg ctgcacgcat ggatacggta actgtgaccg
     3061 aatgtcttga tctggagatt attgcgcaat gcgtcaactc gacaccggac agtattcgcg
     3121 acctcaaccc tgcacttctt cgttggtgta cacctccgac agttccaaac tacttattga
     3181 aaattccctt tggtacgcgt acggcctttc tggaaaatta tgccaaaatt ccacaagaca
     3241 agaaacgctc atgggtacgc tatcaggtca agtatggtga aacgttgtcc gggattgcca
     3301 atcgttatgg gacggacgtc aaagccatac agcaggccaa tcagttaaaa agcagtgtca
     3361 acctaacgac gggccaatgg attcttattc cggtggcgcc caaatcgtat gtagctacca
     3421 aagaacctac acctgtccga aatacaccgg tgataaatgc ttccaatcaa gcgaagacta
     3481 ctaatgcggg tggtaccggt aaaaccaata ccaactggat ggttggaaag aaaaaattga
     3541 cttttaaagt cgaagcgggt cagacattag gtcatattgc cgagtggtat aacatcaccg
     3601 cgcaaaatat tcgcgactgg aacgggcttt attacggcga tccgatattc cccggccagg
     3661 tattgaccct ttatattgat cagtatatgc cggacgaggg ttatcgtaac gcgaccaata
     3721 aacgcactaa agaagtgacg gccgaagagc cgggaatgaa aatatatgtc gtgcaggata
     3781 acgataatct gatcagtatt gcaaaaatgt ttaatgtaga agtgtccaac atcaaacgat
     3841 ggaataaact ttcttccaat accattaagg tcggcgacaa gctaaaaatc tacctcaatg
     3901 acaaacaata agatacctgt cttctgcata gagcttgggt ttttattatc aaagtcgttt
     3961 gagtagatac taattagcct ttttcagcgc ttctatttta accgccaatt caccgatgcc
     4021 ggtatccgtt tgtatatcgg ctgtgaaatt aggatccagc acttgtacgg cggccagact
     4081 tgatccaaaa tcacccatca taaaataatt ttccgctttc attgctttaa gatccgaggc
     4141 tccgatattt ccggcatacg gaaatgacca agtcggctta aggctgagcg ccgtcattaa
     4201 tttttcatta gaacttgaaa actgacggcg tgcattttcg caaaaagacc aaccggcata
     4261 aaagtcaaca tccgtaccga ctttggttga tcctgttata aaagtttgac cggctttatt
     4321 caaactgtcc attttcatca atgcccatcc ggcgcctgtg taaacatcgg cagaatccgg
     4381 atatttcttt atggcatcgg caaatgtgtg atacgaagag tcgtaataag tctggctaaa
     4441 taaaatccat ccttccgaca taaattcggc atacgaattc ggcgatgtat cttccacatc
     4501 cacatcgcac gacacagcgg cgagcattac taataatgaa tatattatac gtttcatgtt
     4561 ttttcccctt atttgatcag aagcattttt ttagtaaaca cggacttgcc tgtattcaca
     4621 cggtatatat atgtacccgt actcacccgt tgcccggttt cattggttcc atcccatgta
     4681 acgcgatggt agcctgaagt tttcggtcca tcccatagtg tttttatttt ttggccaagt
     4741 gtattgtaaa ccgttagtgt caacctatca gaattatttt ccaaggtaaa tgctatggtc
     4801 gtttccggat taaacggatt gggaaagttt tgttttagat caaatgattt cacaatcaac
     4861 gtttcgtcac cgctcgtcgg atcgtattgt aatttaaatc gacctaacga ggagaggttt
     4921 gttcttatta tattttcatt ggccagtaca tgcgattcta ttttgaccca ctgtccattt
     4981 tcttcgcgaa gaataaacca ttttttggac tgctcattag tcaaagcggg caacttgaat
     5041 tgaagttgta gtggcacagt atagttgata tccggcccaa acgcgtaaat tcgttcggtt
     5101 tcttcatttt tttctatcga acatgtaaaa aacatatcgg ttgtcacagc actacccgga
     5161 atcatcaata cggaagcccc gtcttcagta gttaaacggt tatccgaact tggtttggcc
     5221 aaagtagccg tgagataacg taattttgta gaatcttgat ttctgaatga aatcccacgt
     5281 gtttggattg tgtacgttcc gctttgtttt agttgataac tgccagaata ggtatttgcc
     5341 gttccaggta agacgcttag agataccgat gtggtatcgt tggttttcca aaagcgaacg
     5401 cttggaggac taaccaaagc tatattggtg tttacaacaa cgtttatata ttgcggaagc
     5461 actgagtttt gatacaatgc cgtcaccaaa ttaaacacaa taggttttac taagaccgaa
     5521 acttcatttg ataaacgact cattaacccg tccacatcca ctacactcac agcaaaataa
     5581 tatgtctgcc cgttcataag attggtaatt gtttttaccg tatcattttg ggccgaaatg
     5641 ctgtctattt gcgtagtggg attaggtgaa gttcctccaa atatacgata gcgcagaaag
     5701 tcgctatcgt tggatttacg ccaacgtatt gttgcttgag cgctatcgga taatatattt
     5761 agaatcgtcg ttgcaacagg ttttttgtta ggtattaatg cgctggtttc tgtagaaaaa
     5821 tcactcactt gcccatccca atacacggcc tttactctga aataatatgt ccctcctaca
     5881 gtgagagtgg aaattgagcg tgtcgtatcc cgtgaatcta ttgtagagtc catttgtgtt
     5941 gtaggattgg gtgacatccc gtaatatatt ctgtatttga ctatatcgtt cggatcgggt
     6001 gaattccata ctaaatcaac cagcgcacta tcggcaaacg ccgaaattga agtcggcgac
     6061 ttcgccgtta gtggtgtaag agaaaaatta tacgttgaag tagcgtttaa aggcaccgtc
     6121 gtataatgat atgcagtgcg ataacccggt ttgcgcagtt ttaccgttgt tgcaccgtgc
     6181 aatgccgaaa agttatactg ccccaaggcg ttcgtcaaac gtgtttgcgt atcttcgagt
     6241 aaggccacct ttactccggt caaaggtaaa tccgtgttta catcttttac agtaccgcta
     6301 atttgaccgg tttctttgcc gctaaaactg atcattatca atccacgcac tatatcggag
     6361 atagctatat tccctgacgg gaaaaaaggg aaaacacccc aggcgccggt aaaatttgcc
     6421 gaattatccg catcgtacgt atcgtatgct ccggcgacag gcaaggtcga aatattattt
     6481 agatcccaaa ttcgtacgcc ttcggtataa taggaagcat atgcataacg acctttaacg
     6541 taaacattgt ggacgatggc cgtcgtacca aaatctttga cttgcgatat attcgtattg
     6601 tttggaattc catcgcgcag agttgaaata ttccaaattc gtaaacgacc cgccgggctt
     6661 gacgtttcgt ccgtagtgaa aagataattt ccgtcatcag ataatgcagt attgtgacta
     6721 aatgcgttag gataagtaaa ctgatgaatg gtgcgcggtt tagattttac ttttgtgtca
     6781 atgacataaa taatgccctg ataaatcgaa gccgcgtaga tcgtatcatt gcgtacataa
     6841 cagtcatgta cataattttc agcccagttc cccacttcca caggatttct gggatcggct
     6901 aaactgacga ttttaattcc gccgatatct ttaccgccgg gaacgccttg cgtcacatta
     6961 ccaccggtaa ggtacgcgta tccatttctt acctgcagcg catgtgttct gaaataattt
     7021 ggcgttgtcc agttttgaac taatgaggct gaggtcggca attgagataa atcaattacc
     7081 tgcaaaccgt taccgccttc agatacgaca taagcatatt tatcccaaac ctgaacttca
     7141 tgccatgaag aacttggtgc aggaatgtaa gcgatttcag ttggagaggt ggggttggta
     7201 acattgatga tcgctgtacc gttgaaacat cccacgatag cgtattcgtt tcccagcgag
     7261 tcaacatacc cccagaggtt agcatagttg cttccgtgat cgtctttttg gctcaaaaac
     7321 gtcatatttg tttgtgcgat gatgacacct gaggaataac ttaaaagaaa aactaacccg
     7381 gccacgtaaa gtttttgcat aacagaactt tcgcctttga tattgataac gaaatctatc
     7441 cgataattaa acgaaacaaa ccaataagaa ttccaatcgc aaaaccggct acggcgccat
     7501 tgacacggat ccactgcata tcatcatatg ttcgcgaacg gaactgatca cgtatctcct
     7561 gaacagataa tttattgatg ttataactca ccaattctgc aatcttaccg tgatgcgctt
     7621 gcagcatatc ggacgctatt tcaataaaac gggtgttgac ggctatctgt aagctacgat
     7681 ctgattctaa cataaaaatg gtattatgcc aaatgttttt taacagttca tctatttttt
     7741 ttccgctatc cgtttcacta tccaataatg attttaattt tactttgatc gccggtatgt
     7801 attgtttcaa atctgtttca cttataatct cttgtatcca tgtgtcccat gtattgttgt
     7861 cttgattctt aagcgtttgt ttccatgcca tggaacgttt tattatttca aggcgcaatt
     7921 catgattttc atcggcggct atagatctca atatagatgt cgctttggta atgatgcgtt
     7981 cagttaattc gccggcgtca aagaacagct tagaaaaaaa accttgttct ctttcaatat
     8041 tatcgcgcaa gagttcatac acggtttgtg aagtcatggt atcggctatt ttttcaggcg
     8101 catgatcaag caagcgatga agaattttag taaattgttc gtccggtaca gagtgaccca
     8161 aaaaatgtat cggcttaatg ctttttaatc cttcgcgcaa taacatttca aaatatgagt
     8221 tcgattccgg cgaatccagc cattgaataa ttcgcgcacg tgcccaattg ataaaacgat
     8281 ccagctcacc attctgctcg atttttttta attgaacagc taatgccgat gcggcatcaa
     8341 atgagcttaa ccatccggtt atactgtctt tggataacca ttcttctgcg actaatttac
     8401 cagcgccgtc agcgatagat ttctgttttc gcggaataat accggaagcc ggcacaatac
     8461 gaaagcggcc aatcttgatt tcatcaaaaa tcatctttac agctagccaa tctgctgctc
     8521 cgcctactgt accggcttca aaaccggatt gcagaataaa taccaacatc ggtaaaaaat
     8581 gaggatatga agccgcgata aaaaaacacg acagcagtcc ggtcaccata atacccagcg
     8641 acaattcgcc tttgtagtgc tcaatttttt ttaaaataat cattatggct atttcgttct
     8701 atcgcggaga gtaagaaaat cttgatatat aagattagat agacgatcta tattttggtc
     8761 tccatccaga cggaatacgc gtccggattc tttttcagcg agagctaagt atccttgacg
     8821 aacgcattca aaaaaatccc gcccttcgtt ttccattcga tctttctgcg acgcgatgcg
     8881 ccgcttctgg ctttcatccc atgaaatatc aatcaggtat gtacgatccg gtgaaagacc
     8941 ttgggtagcg atatggttag ctgcgtggat cagtgctaaa tcaatttttc gcccatatcc
     9001 ttgataagcc gtcgtcgaat caaaaaaacg gtcaattaac acgacatatc cttcggccaa
     9061 cgaaggtctg atcttttccg ctaagatctg agcacgcgcc gcttcgtaga gcaacaattc
     9121 cgtaaccgga tcaatgacgc tgtcggtaga taacaagatt tgacgtattt tttccgatac
     9181 agtagtccct cccggatcac gcatagcgat gaccggcagt ccctctgctt cgatacgcat
     9241 ttgcaaacgt cgaatctggg tggatttacc gcagccgtca attccttcga aagaaataaa
     9301 aataccacga ttcatgtgcg tgattccata aaatggccgg ttactttttc aaatagttgt
     9361 gtcaagatag gtcccgatgt gccttgaatg tggtatgcgg cttgttcaga gataggcgtt
     9421 gtttcagggt ttatatcaat caatagcgcc cccgattgtc gcgcatgtat cggcaacccg
     9481 gccgccggat atacgacacc gctggtaccg atgcataaaa aaacatccgt gtttgatgcc
     9541 gcatgcatag actgttcaaa aattttttcg ggtaataatt ctccaaacca aaccacgtcc
     9601 ggccttacat agccacagca tctgggacat agcggtggta tatcatctgt atccaaagga
     9661 tgctccacaa tcttatcatc cgtaaagcac ttagttcgaa aaatattacc atgaagttcc
     9721 aatacacgtg tactgccggc tacggtgtgt aagttatcta tattttgggt tattgtcgtc
     9781 acttcaaaat gtttttccag ttcggatatc acgtaatgac ccgcgttggg ttgagccgct
     9841 gcgatcattt gacgtcgcat cgcgtaccat tcccatacca ttttgggatt acgtttaaat
     9901 gcctgggggg tggctaattc ttccggttta aaatgcgccc atagaccgct ttgtgcgtcc
     9961 cgaaatgtcg gaacaccgct ctcggcggac atcccggcgc ccgtgaaaaa aacccaacgc
    10021 ttagcttgtt gcagacgacg aataagcgct tcatctatca ttacgcgatg gtctccaaca
    10081 gcttttgtac cgcagtataa aatggtttta cggtcggtat gtggagacct tcatccggcg
    10141 agtgaacgtt acgaagggtc ggaccaaaag aaatcatatc catatcggta aatttttcac
    10201 cgataatacc gcactccaaa ccggcgtgaa tagccgttac tttcggattt ttaccgtaaa
    10261 tttctttgaa gacttttttg gttatagcca gaagcttcga ctgtggattg ggtttccatc
    10321 ccggataacc gtcggtttct ttcaccgttg catgcgtcaa cgaacaaatg gcgccaacct
    10381 gatctaaaat gtcgcgtttt tcttcttcga tcgcactgcg atgactggaa cctattatga
    10441 tctctttttc gtgtgtttgt atggttgcaa gattaacaga tgtttcgaca agaccatcaa
    10501 catctttact catagcttgt acgccgttgg gcaatgattt caaaagtcga atcagttcaa
    10561 cggaacgcga acgtttaatc gtactaaact gagcaatggg cgcttctttg aaaacaattt
    10621 ttagatccgg atcggtactc tgatatttcg cactgaggta attatgcatt ttttgaacgc
    10681 gcactttgaa cgcaccgata tcttccttgc gtacggctat atgcacagcg gcgtcacgcg
    10741 gaatggcatt acgtacggtt ccgcctttga tgtccgataa tttaaatttg tactctttgg
    10801 tcaattcgta caacacgcgc gtcaaaattt tattagcatt agcccggccc aaaataatat
    10861 ccataccgga atgcccgccg acaccacctt caacggcgat aagacagcgt gtttcatttt
    10921 ctttagccgg tttacgtttg atcggaaaat gaataacacc gtccttcccc cctgaacaac
    10981 cgatgaatag cacaccttca tcttccgaat cgagattgag caaataacgc cctttgagca
    11041 tggaaggctc caatttcaaa gcgccggtaa ggcctgtttc ttcatcaacg gtaaatagta
    11101 attctaacgg cccatgtttc atggtcgtgt cttcaagtat ggcgagcatt gtcgctacgg
    11161 caattccgtt atccgcgcca agcgtcgtgc cggttgcttt gacccattct ccgtcaatgc
    11221 ggggtttgat cggatcgtta tcaaaatcaa tttgagtatc tggcgttttt tggcaaacca
    11281 tatccaaatg cccttggaga acaacaaccg gtttattctc gtaaccggga gcggccggtt
    11341 tacgaatgac aatattccct gcggcgtctt cactccagtc cgaattgagt ttttgtgcta
    11401 ctcccttaat atacgctcgg attttttctt cctttttcga aggacgtggg acttgagtta
    11461 tttcataaaa ataatcaaaa acgcgtttag gctcgagatc ttttaataca tgatcagaca
    11521 tatttcttct ccgttattca tataattaac cgcacggcaa gttaggttat tcgactaaaa
    11581 attccaatat ggaaattcgt tttgcttata tgcattgcgg taattacttt aaaaaaaagg
    11641 aaggccgccg atgaaactca aaaaaatact gatcgttgac aatgatcccg cgtcattagt
    11701 taatttatcc gctatggtgg aaaaagccgg ttttacccct gtcggcttcc agaaaccaac
    11761 cttggcgatg gagtttatcg aaaaaccgga caatcatgat attcgtatga tcatcgcaga
    11821 gatgaatatc cccggctctg acggttttac attccttaaa gccatacaaa aaaaaccaca
    11881 actcaaactg atgccatttc ttttcttgtc cgagctccaa gatacagccg tgctggtgaa
    11941 tgcctacgaa caaggtgctg ttgattattt catcaaaccg attcgcaaag aatcgttatt
    12001 tattgccaaa atcaaatcca tggtggacgc atttgagcgc acactccaaa gcgccaacac
    12061 gttattaagc ggccaactga gcgataaacc attagtggaa ttactggcgg tatgtgaaaa
    12121 cgaatcctta aacggctact tacgaatcaa acacgccgat gggatcggag tgataacgtt
    12181 tgtgaaaggc ctaccggaaa aaatggctat cgtggatgca cagcaaaaaa tcattaaaag
    12241 tgaatcggaa gcttttgaag ccatgtcgga ttggtcggag ggtgaatttt tgatacgacg
    12301 tggaaacctc tgatagaggt acgtcatttt ccgggcggta tcgcggaaga atacattgat
    12361 cggaaaatca tatcggccgc gtcttgcagg ataatttttt tgtaaaaaac agcgtcaatt
    12421 tgttcatcta ccaatccacg atatccttcg gaagtaaggt ggtcgtgtaa ttttttattt
    12481 acgccacgca gtaatgcgtt tcgaatacga ctcttccttc ttatatccat gtccccgctt
    12541 gattccaaat attctctatg cttaaaaacc gtttctaaaa cggcatcaat accttcgttt
    12601 tgcgttgcaa ctgtttttaa aacgaccggc atccatggtg catgggtgta acgaatttgt
    12661 acggcagctt ctaattcacg tttgaggatg tccgcctgat cgcgatcgga tttattgact
    12721 acaaataaat ccgcgatctc cataatgccg gctttcatgg cctgcacccc atcgcctgat
    12781 tccggtacaa gcacaacaac tacggtatcg gtgtattgga cgatatctat ttccgattga
    12841 cctacaccga ccgtttcaaa aataataaca tcgtacccaa acgcatccaa caagtctgcc
    12901 gcggcgtgcg ccgtatgcac cagaccgccc atcgccccgc gggtagccat actgcgaata
    12961 aaaatattct catgcgtaga aatcgcttgc attcgaaaac ggtcacccaa tatggcgccg
    13021 cccgtaaatg cgctggtcgg atcgacggca ataataccga cacgtttccc ttgttgtgcc
    13081 aatcctaatg cagcggcatt gactaatgta cttttaccgg cacccggagg gccggtaatg
    13141 ccgatacggt aggcttgtcc tgtataggga agtaacgctc gcaaaatatc ggggccgttt
    13201 tccgcgtcgt tctctaccga cgacagtaat ctggacagat aacgttttcg tttaggatca
    13261 ttatgcgcta ataaacgcac ggcatctata tgttccattg agatactcat atgctaattt
    13321 cgaatagccc cggtcaaaat acgaaaagca attcgcatca gatcggcaaa aaaataaaac
    13381 ggaatagtca accaatgata ccaacgggca tatttgatga gcaatttcat attactaaaa
    13441 aaacgtaacc gaatttttcg taatgataat tgcccacccg tcgaagcgga tattttatgc
    13501 caaatttttg cctgcggaat atacaccaat tcaaagccgg cctgttttgc tcgaaagcaa
    13561 aaatctacat cttccgaata catatgatat gaagtatcga gtcccggcac gctcttaaaa
    13621 acatcgcacc gaatcagcat cgcacagccg gtgatgtaat cgctcgtttt tgcttcttcg
    13681 tattgtaggt tttctttttc acgaatgcca atatgacgcg ccgtaccttt gtataattta
    13741 aattcggctc cggcaaacca aatctgatca ttcggttcaa agtagtagat tttaggaccc
    13801 aaaataccag cttgcggata gtgcgccagc ccctcggata gatgaacaag acactttttg
    13861 tccatcacag tatcgttatt caacaataaa acagcatcgg cgttgttgtt cagcgcgtat
    13921 tgaataccga cattattccc tcccgcaaaa ccgagattag cttcattacc gatgtatttg
    13981 atttttccgg agtgaatttc agaattaaat ttttctttca gataatcgcc gcttccgtca
    14041 ggagacgcat tatcaaccag cacgatagta aaagatgtga aatcggagtc taagcaagag
    14101 cgaacgcacg cttcggtgag atgccgtccg ttatacgata agatgattac aaaaatatgc
    14161 ggcgtttgca tacatactac gggttatcga ccatcctgaa atgcaatatg ggataacggt
    14221 actgtgacgg taaacgtcga acctttacct tcgacgctgg ttacgatgat ttttccgcca
    14281 tgtaattcta cgattgttcg tgcgagtgat aatccgatac cggcgccctc cgccgaacgc
    14341 gtcgtggtct gatccacttg ataaaattta tcaaaaatac gctcaacata tttactcgga
    14401 attcctgaac cggtgtcatg tacatgaatt tcaatagcat cggtagtcat cagcgcatgc
    14461 atacctactt taccgccaaa acccgtaaac ttaatggcat tttcgatcaa atgaccaatg
    14521 gcttgtataa aacgaggacc atccatagcc acgatgggaa acgtatcttc gactttcatt
    14581 tccaagttaa tatttttttc gcgggctatg tctttatatt catcaaacgc cttgctaagt
    14641 aattctttga gattattggg atggcgctcg attatgatgg aaccgtattc gatctcagcc
    14701 aatgaaatga gatcattaat aagatcggcc aaacgcttac tttcgtgtaa aataacttcg
    14761 acgaaacgct gtttcgtttc atcatctacg tctttagatt ctttcatcaa cgtactgaaa
    14821 ccttgaatcg aatgaatcgg cgtacgaagt tcgtgcgata tggttgatag aaaattagat
    14881 ttaagatcac gcacttcctt tcgcgcacga tacccccaac ttacgacttc acggaaaaga
    14941 ttattggtat ccctcaggcg tttgctcatc actttggcta aacgcgtcat gatttcatgc
    15001 gacagtttta tgtcttcatg cattaatgca tgaaacgatt cttttggaaa aaacaataat
    15061 tccgtttcat acatcgtcac cgcaccggcc gaacgcactt cttcatccaa taacgccatt
    15121 tcaccgaaat aactgccggc gggaagtaca gctacggctt gttggcgcac ttcttcaccc
    15181 ggcacgcttt tgaaaatttt cacgaaccct ttggcaatga tgtacatacc gtccggctgc
    15241 gtattaccgt cttcaaaaat caccgtattg gtcggataca ctttgagcgt aaaatgcggt
    15301 aataaacggc gtagtgcatc ttctggtata tttttgaaaa gcgcagaaga cgctaacgtt
    15361 gtaagtttgt actcatctga ggacataagg tgttccgtat tcggtaaaat aattatgtag
    15421 aaacatattt gttattgagg gcatttgcaa gcatttaggt cttaataacg ccctctatag
    15481 gactggaagc cgtagcataa agttttttcg ggattcgccc cgcttcataa gccagacgtc
    15541 ccccttcgac ggccaaacgc attgcgcgtg ccattttgat gggatctttt gctccggcga
    15601 tagcggtatt catgagaata ccatcgcaac caagctccat tgcaatcgct gcatcggaag
    15661 ctgtaccgac acccgcatca acgatgacag gcactttgga atgttcaatg ataatagcaa
    15721 gattgtacgg attacgaatt cctaaaccgg aaccaatcgg cgcagctaac ggcatgacgg
    15781 cggcacaacc gatgtcttcc agttttttac aaacgatcgg gtcatcaata caatatggca
    15841 aaacggtaaa accatctttg actaaaattt gtgcggcttc gagaagctgt tcattatccg
    15901 gaaataatgt acgggggtca cccaaaacct ccagcttgac caatgtcgca cctaaggctt
    15961 cccgtgccag tttacatgtc agaaccgcat ctttagccgt gtaacaaccg gccgtattag
    16021 gaagtaacgt gtattttgaa ccgatataat cgagcagatt cttgtcgtcg ggtttagtga
    16081 gatcaacacg tcgaagcgcc acggtgatca tttctgcgcc actctcttca agcgcttttt
    16141 tagtttcatc gtaatcttta tattttcccg taccgacaat caaccgcgaa ttgaaggaat
    16201 atttgccaat taccaacgca ctcatatttt attttcctaa atattctttt ttgaatatac
    16261 gcgttttaat aaaaatagca aaccccgccg taaagcgggg tttatataaa ctaagcttca
    16321 gccgccctgt gttgctcgta tgatttcgat ttcatcgccc tcgcataccg tttgtgtttg
    16381 aatgcgatct ttggaaatta cgtcgtaatt aacagctacg gcgacaccat gtgcatatga
    16441 aacaccaaaa tgttggagta catcatcaat tgtttttaca gaatccaatt cgtaaaccgt
    16501 accattgatt ttaatcttca tagctaagcc ttaccgttaa tccgacgttt tacttttcgc
    16561 aataaatgcc accgccacac agggttcaaa taatcggtaa ataagacacc gttgagatga
    16621 tctatttcgt gctgtacgat acgggccttt actccgataa aacgcttcgt ctgtgatacg
    16681 ttatccatat cggtataact aactgtaact gcagcagggc gccttatttc agcctcaacc
    16741 cctggaaggc tcagacagcc ttcgcgaaat acattggaac cttcgctttc gcgtatttcc
    16801 ggattgatca tcacgatcac ttcttcggtg cgatccatta ggcgcggatc aatcacgaca
    16861 agacgttcac tcatgcccac ttgcggtgcc gccaaaccga cgccttggtg ctcaccaatc
    16921 gtttcgatca tattcgcgac cgtcatgcgt aacgtgtccg tgatagcaac gaccggttta
    16981 caaactgaac gcaaaatggg atcgccgtat aaaaccactt tcacaatgaa atcggttatt
    17041 cggccgattt tgcttcagcg gatgcttcgc cggaaccttt gacaacggtg gttaccgccg
    17101 aacgttcgat ttcgactttt acattatcag ctatttttac caataccgtc gtctctttgg
    17161 taccttggac tacaccatgg ataccgccga ccgtaattac tttatcccct ttcgccaaag
    17221 cgctcaccat cgcttgatgt tctttttgac gtttttgctg aggacgaatc atcatgaaat
    17281 acatgatcgc aatgataaat acaaaaggta aaaaactcag caaaggattc ggtgcggcgc
    17341 cgcccggagc aggattaccg cccatcaacc acatcatcat tattggattc aaagtacacc
    17401 tcatttagtt aagtgaatgt atatttagag taaactaatt ttctcgccga gtgatttctg
    17461 aacattcgaa agcatccagt catgtaaaat accattggaa ccgattacat gacctttgta
    17521 caaaaaatta ttttgtccgt caaaaccggt aatacgtccg ccggcttctt gtatgatcaa
    17581 tgtaccggct gcgatatccc aaggattgag gttgagttcc caaaatccgt cgaaacggcc
    17641 gcaggcaaga taacataaat ccaatgcggc ggagccgggt cttcgaatgc ccgccgtttg
    17701 cgtcatgaaa gttttaaata tattgagata atcgtcaata aaatcaaacg tcttgaaagg
    17761 aaatccggta gctaaaaggc aacgactatg ttccgtagtg gcagacaccc gaatcttgcg
    17821 gtcatttaaa aaggcgcctt taccgtgttc ggcgacaaac atttctttgc gcaacggttc
    17881 atatacaacg cccacggcta actgaccgtg cacttccaac gcaatgctta cggcgaacat
    17941 aggcattccg tgaataaaat tggtggtacc atccaatggg tctataatcc atcgaaaatg
    18001 gctttcgtta cgatgtttac cggtttcttc cgctaaaata gcatgatccg gaaaagcacg
    18061 taaaatgtgc tgtatgataa cttcttccga tttacgatcc acttcggtaa caaaatcgtt
    18121 atgcaactta gcatcaatcg taaaaacgcg cccgtaatag tcggtaataa tacgtgccgc
    18181 ctgtaatgcc gctccaatag cgacgctgcg cgcctgtcgt ggaatttcga tcatcatcag
    18241 ttctttttca gaccttgtat ttgttcgatt cgctgcttgg cataggcgct gtattcgctt
    18301 tgcggaccat tgacacgaat aatatcgtta tacattcgta tggcttcatc gtatttggac
    18361 aatttcacgt aacattccgc cgcttggctt ttagcggcac tggaccaatc gaattttttc
    18421 cccaatgtca aatatgggat gcgtaagtat tccaacaggg cctgttcgaa attattcatc
    18481 gcccaatacg tttctgcgat attaaattga atttccgctt cgtcttcacc ggaagccgtt
    18541 ttaatgagat ctttaaaata ttcaagtgca cgatcaaagg acttcaagtt tttgtgcatg
    18601 gcgccgatgt caatacgcat ggcccaaatc gttgccggat cgtcgtccgg aaaacgttcg
    18661 agatattgct gcataagcat caacccgttt tcataatatc ccgcggcttt atacactttg
    18721 atcaaaaaat tagtccccaa tttggcatcg gctaatttga tacgtggatt ttgaagcgcc
    18781 aacttagagg cctcaacggc gagttgaaca ttttctagac gaaaagcttc aaaagctgtt
    18841 ctcagatgga cacgcggaag tatgtcacta tccggccatt tttgcggaat ttgctgaaag
    18901 tattcaaagc cttctttgct cttgccctca ttaaacttga tcacacccat ttcaaacaaa
    18961 acttcaggca tgatggccgc tgttttataa tcttcgatca cacgcgtata tgtttttaca
    19021 gcttgagcgt aatcggcgcg tgttttattg tattgataat aacgacctaa ttcgaataaa
    19081 aattctgcgt tataattcag caataagagt ttaaggtttt tatccgattc gtatttttta
    19141 ttaaagtctt tctctaactt tccggtttcg acgatttggc ccagacggta tgcgcatacg
    19201 atcgtgcgtg attcaaaaac aatttgctgc attttatctt tggtatataa cgctagcttc
    19261 gtaaacgcta ctttggccga atcgtattgc tcggaattga agtacatctc cgacgcccgt
    19321 gccagcgctt tgaatccggc ttcggtttcc ggatataact gcgcgatacg ttgataataa
    19381 ggcagtgcaa tctgaaaatc ttgcttcttc tcatacatct gcgcaagcgc cgtcagggca
    19441 tgcaacgcgt attcgccttt attatccgga aaaagatacg cttcgtagta ctccaccgct
    19501 ttggtaaaat taccggattt gtcatagcaa taagccaacc ggtaatcaag cggatgatgt
    19561 attttttgtt gcgcggcaat aatatccatc tgctccaatg caaactcgcc ctgtgtgcgc
    19621 gcattaagat acgctttgat cgcttgggta tattggcctg acagaagata gttatgtcct
    19681 accgattgag cagcactatc ggcatacggt gtataaaaat aatgcgtgat gatctcattg
    19741 agattattca aagcaagcgc ataatcggac ttttcctctt tatagcgcgc cataagcatt
    19801 tttgttttga cgatatagcg cccgcgtgga tattgtgccg cataccggat caaagactga
    19861 taatacaaat ccggttgttt ggaaaaccaa tacgcttcgg ccatactata aaacgcatct
    19921 tcggccacag ggttgtccgg aaaatctttg ataatcgctt cataacattg catcgccgaa
    19981 ctcaatggtt tcagtgaatc gggttttgca gtaacgggtg atttatcttt acttttggta
    20041 acggaaggaa cctgtgggac aataacaggc tttaattcac cgagcgccat catcgcatca
    20101 cccaatcctt tgagcatgtt cggctttagc gttgaaccag gaaatcgttc cagcaatccg
    20161 gcgtacatct ctttagcttt tctctgacga tcggtataat tactaatcgt tttcagtgtg
    20221 ctttcgatga cacgcacggt ggcgacatcg gcatatttgc ctacggctaa ttgtttgtac
    20281 acaccgacag cgctgtccgc ataaccggcc tgtgttggct tcatttctga tatccggtca
    20341 taggattgag cgagataaaa taaagcttct tccgtcaaag cgcggttttt agtcgagagg
    20401 atggtttttt tgaataatcc tgccgcaccc tcataatctt tgagttgttc aaagaataaa
    20461 cgcgcataag caaatgtgat ttcgtctttc ggcttatctt cgataaggtt accgatcaga
    20521 taaattatac tttgcgtagt cgtattatta ccgtattgat gaaaatattc gatacgtcgg
    20581 cgctcatcca gggcatcatt ccagagtaat gttccgggat aattgttttt aagtttctct
    20641 aatgcatcaa gagcttcaga tatctgatta tccatacgca acgactgcgc taaagcaagg
    20701 tacgcgtcat ctattttttc actgttcgga aattcgctga tcaggcgcgt aaacatgcgc
    20761 gatgcttccg cataatcagc taaccggtct ttgagcaata taccgcgatc atataaaacg
    20821 cgatctaaat cggcagattt agggtattta gtgatataat cttcataata acgacccgct
    20881 tctttatagc gttttagttg aaatgaaatt tgcgctaact tgagtgtcgc ccatttgaca
    20941 aattgggatg attgaaaaac tgaggcatat tgttcggtga tagtataata ataatcaccg
    21001 gcggcgaccg ggaggttcag tttttccgac gccaaaccgg atttaaaatt ggcctctagt
    21061 gcatttagac tatcgttttt ttcggatgca acgcgtttat acagatcaag cgccagagta
    21121 tatttctcat tgacgaaaag tatatcgccg agattgatct gtgcggcatg tttgagcggc
    21181 tccggagcaa gtatattgga aatgacgatt tcaaagtttt tacgcgcatc aataaaattg
    21241 cgctctctga atagaatatt gcctaattcg aacaaggcat atacgttgat cttggtctta
    21301 ctgtatcgtg ttacgattcg gacataagca tttcgcgctt catcggattt accaaggcga
    21361 tccgcgattc ccgcctgttc aaacatggcc gaagccacca attcctgatc ttttgatttt
    21421 tcaatgaccg tttgaagtgc accggaagcg gccggccaat cgttttcatt tttgagaagc
    21481 atcgccagtt tgaagcggct gacattagct tccggaatat cgggacgatc ggcgatgagt
    21541 ttttgcaaac gatctttagc gctgcgcaga tcgccggctt tgataaaata ttcactggcc
    21601 tcgataatgg acgttacggc attgggtgcg gtgggataaa atgttgcaat actcgtaagc
    21661 gcattggctg cagagggata atcccccatt gccgcataac attctgctat cttggcccac
    21721 gcatccggcg cacgtttatc tttgggataa tcaatcgcaa gaaattgaaa gtcacgtcgc
    21781 gcctgttcat aatttttttc attaaactgc gccatacccg aataataccg ggcatcagga
    21841 atgcgcatat cggagggata atttcgtata aattttccga attcctgcgc tgcgagcgca
    21901 tagagcttat catcatacag cttacgggca tataaaaaat cggtatcggc gctcgacact
    21961 tgtgtgtgtg ccggcgtaga aaggatcaac gaaagtatga tcgtaagcag gatagatgaa
    22021 tttcggatgt tcataacata gtctttcgta tgtacactat ttgaccatta ggttggcgta
    22081 ttttaccatc agacggcgct ctactccatc accaaaaaga atcgtgagtt tttgtttgtc
    22141 gccttggcct tcgctgtttt ttatggtgcc ggttccaaac gaagcatgga taacttgctg
    22201 accgatacga tatttagttt cagtgatcgc agccgatgtg acaatttcat ctgaaaaatc
    22261 tttcaacgat tttttgacac gtgaaggacc gtcttcatac gctgtaacgc gttcggaacc
    22321 ggaatagttt ttggattgcc aggatttccc cgacccttcg aatagccgcg gctcataggt
    22381 tgaaattttc tgccaatcaa gcaatttagc cggcacttct tcaataaaac gagaacgatc
    22441 ggtttgataa atgttattaa accgcatacg ccgtttggcg aatgataaat ataattcatt
    22501 ctgcgcacgt gtggtgctta cataaaacaa ccgtcgttct tcttccagtt cgcgcgcatt
    22561 attgcgtgca ttctcaagag gaaacaaacc ttcttcaaga cccgcgacca cgactaatgg
    22621 gaattctaat cctttggcac tgtgtatcgt catcagggta acggcgtttt tgttttcatc
    22681 atatttatct acatcggcaa ctaatgccac ttctttcaaa aaggcttcaa gtaccggttc
    22741 ttcagcttgt tcggcatcgt atttcataga aaactcagat accgcattga gtaattcgac
    22801 aatattttcg taacgcgaaa tcgcttcaac tgaattttcc tgttgataca ttttaagtaa
    22861 cccgagttcg tctattaata tccgtaccca ttcgccaaaa cccatctgcg tgcgtaactc
    22921 ctgatacttt tgaataaatt cgcaaaatga cacgatcgcc gccatcgtgc gttcctgaat
    22981 caaccccgca ccgaaagagc gcacattgcg aaacgcatca aagagcggca tgtttttcac
    23041 ctgagaaaac tcctcaagtt tttccaatgt cgttttaccg ataccgcgtg ctggaaaatt
    23101 gatcacacgt tgaagactga tgacatcggc cggattggag atcagcttga gatacgccat
    23161 cacatctttt acttctttgc gatcataaaa acgaactccg ccgacgatga catacgcgat
    23221 agacatttga cgtaacgcat cttcgagtac gcggctttgt gcattcgtgc gataaagaac
    23281 ggcaaaatcg gcaaaacgaa ttttttcttg ttgaattttc ttttggatca cttgagcgat
    23341 gttcttagct tcggcacgat catcatcgca ttcaaacacc gtgatcagct taccctgtgc
    23401 tttatctgtc cacagcgatt tatctttgcg tatttcattg ttacgaacaa cttcgttagc
    23461 gacttgaaga atattcgctg tagagcgata attttgctca agtttgtaaa cgctggcttc
    23521 agggtaatct ttttcaaaat ttaaaatatt accgatgtcc gcatgacgcc atccatagat
    23581 agactgatca tcatctccta caacacacag attgcgatac ttagcggcaa gcatcttcat
    23641 aagaatatac tgtgcccggt tggtatcttg atattcatct acatggatgt agtgaaattt
    23701 ttcctgatag aaagtcagcg tttcgggaaa ttgctggaaa agttcaatcg gttttacgat
    23761 caaatcatca aagtcaaagg cgctggcttg ccgtaatcgt ttttcataat ccgtataaac
    23821 tagcgccgct ttttcatcca tcggattatc aaccgactta tgaaattgcg atggattttg
    23881 aaatgtattt ttggctttat tgattttggc gcggataata ttgggcgaat ataattcctg
    23941 agaaatgccc aaatactcca tcgaaatttt gactaaattg agctgatcat ccgaatcgta
    24001 aatcgtatag tttttagaat aaccgatttt ttcagcttca ttccgaagga ttttcccgaa
    24061 aatagaatga aaggtgccga tccatatttt ttgactatga tacccgacta atctttcgat
    24121 acggtgtttc atttcaccgg cggctttatt ggtgaaagtt acagctagaa tattaaacgg
    24181 gctgatttgt ttttcggcta taaggtaagc aatacgccgc gtaagaacgc tcgttttacc
    24241 gctgcctgcg ccggctaaaa tcaaaaccgg cccatcggtt gtttggactg ctattttttg
    24301 agcatcgttg aggtttgttg tcaaaccgtc gccggacata ttttttcctt atttattttt
    24361 tggcgcgtgc aatgtataga acgagctatt gaaatgcaag gtagcaaaaa atcctgaccg
    24421 gtagtgatca ggattttttc tgaacaattt attttgagtt taacgtacga ccatcacttt
    24481 tgtcgtatat tttccttcgc cgccggattt cagtacggca atgtaaacac cgttacgcac
    24541 tttttcaccg tttccgttac ggccatccca aaccatttcg acttgtgtat cgtctacagt
    24601 attttgtgtc aatttggtta cgagattacc ggccaacgta taaatttcga taaccgcttt
    24661 ttcgccggtt cctctgaaac ggaaagtcgt cttttctttg ccgctactgc caaaaggatt
    24721 ggggaaatta ccgaattgtg caaccttgcc gccattctcc ggatcctgta tgcccatttg
    24781 ttgcgttgta ttacccgcat tggcttccgg tgaacccaaa agattgcccg tagcatctac
    24841 ggcaggaagc agcggaagat ccgtgatgcc ggcatcttgc gagcccagta catcattgtc
    24901 atatgctgtc acgcccacca atatcaatct gaaatttggg tttttgcctg caacagccgg
    24961 ttttaccctc gctttgaata caacttcttc ggcgactccg ggaagaatgg ccaacccgta
    25021 atctaaatcc acagccaacg cgcgggctag tttcttcaat cccgcatttt ggtccacacg
    25081 gacgaccgcc gtatcttcat cggtacctag attgtttgca cgaccgaaac taacctcttc
    25141 acccatgagc cgcaccgtga tcgaatcgaa ataagcggtc cagttggtgt cgttatacgt
    25201 gcgcgttttg taatcaaaag tttcaactac aaatttaaat tcctgaacga tcattccgtt
    25261 ggtgttggtc gtggaagaat tattcttcaa ccgcaatgcc atgatgctgt caacaacacc
    25321 gtttacgtaa atcgttgtac ccggatccgt tgcgatacgt tccactcgaa gttgtttagc
    25381 ttcggtagaa acggcaaggc ggaatgaacg cgattcaacc gtcacggtat cgcgtgtatt
    25441 cgtatccgtt ggtaaatgga caaagcgcag taccaaagga tgattaacta caccatccgg
    25501 agcacgaaca agccacgatg cggtaaaatc accattgtca aaatcaaata tgttgtctcg
    25561 gtttgccaca tcctgctctg taaagatcaa accattgccc gcatctaacg aatccaaaag
    25621 caacatacct tggccgcgcg gtaacgcata accgagattg gataccgttg cctgtatgcg
    25681 gaatgtttga ttttttgtga caaaattgtc tatagcatct accggctcga tgatcgcagc
    25741 acgtaaacgc agaacggctt tacgttgcaa agtaaagttc ttatcaacct gcgccgtcac
    25801 attgctgtct ttgttgttac gatcaacgcc gacaaatgta atccgggcca agtcgatctg
    25861 ttcgttatcc gtcggaatga taagcgttga aggcgccaca acactccagc ggacaatatt
    25921 atccagatcg gatatttgtt taagtactac ttcaccatct ttcagacgat acgcggagtt
    25981 attcggaaac tgtatcgtag cgtaacgcgc cgtgagcgaa cgcgactgag taaattgtgc
    26041 gctgacgaca aatatctgat ctgtcgaaac cgaatcacca ggaacacctt gtatcgttat
    26101 ggacgcgtta aggatttcag ccggattgac aaccacgaca ttttgtgtgt ccgcattgct
    26161 gaaaacaact acgggttgat ctgtattttc atcgttgggt aatacattta ccacggctgt
    26221 catttgtaat gaaccggatg cgttagcacg taatgtccag ttgatcatcg tatcgcgtgt
    26281 aaaagtatat acattaggag ccgcaactac atcaaatccg actggaacat taagcgtaat
    26341 ctgaccggca ccggagaatg ccgcttgacc taaattacgg atcgtcgctt cgacgctaag
    26401 ttcctgaccg aaggacagtg tatcatccaa tgcacctaca ggcccggtga tacgcagtgt
    26461 cgttgtcatt tctgcacggc gtacaaccgt cacagacagt ttgagtgtgt cgcttatgac
    26521 cgtgtcaccg gttgtagaat ctatacctga atacacaacc cgtaacggat aaacgctgtc
    26581 gttatccgta ttggagaata tccgccatgt cgtaacaacc gaatcattta cattttgtat
    26641 aaatgcctgc gctaaactat cacgggtaga cagaccttca cttggtaaaa tcatccgcgc
    26701 ttgcataccc gagagacggg atagatcttt tactaccgtt tcgactgtaa atccttggaa
    26761 tgtcgaaacc gtcccatcaa cggcgccgtt cgggcttagg atacgaatgc gcgtagcaat
    26821 cagatttccg gtattgctgg ttttcaggaa aaccgagtcc cgcacagtat gaatagcgga
    26881 tttactattg gtattaacgt ctaatggaaa tgcggcatta gctgtatcca aaagtgcaac
    26941 taccgagtca tagtccggcg tttcttggta cgaactcatc ggtattgtaa atgtgcttcc
    27001 gaccgtcaca aaataaactg aatctaatgt gggtgaagcc tggtagcgga acatattgtt
    27061 tatcggtaat cggacacgca caggaaccaa tgccgtattt aacgaagctt caccgatatt
    27121 tgtgaccgtt acacgcagat taaaattcgc cgtatccgac acttcgagcg agtcaccggg
    27181 tatatcaaca aataaattca atttcagttc ggccgaatcc tgcagagtaa acgtcacttg
    27241 atttccggaa atcggatcaa caatgtctaa tggcctaccg gaaatgagtc cgtaaccaga
    27301 atcaacaaaa gcacgataac tttctgcgcc ggatgcatta cgtgttctga aataacgcga
    27361 cacggcccat agcgaatccc ctccgttcag cgtcgtcgtc aacatactgt cgagatccgc
    27421 attacccgcg tcgctttcga aatgcacgcg gagattagcc gactcttcac caaaatttct
    27481 aaccagtgat ttgatccgaa caatctggtc acgattcaag atgcctgtat tgtaaaccgt
    27541 atcaatatac gtgcgcgtga tctgaagttg tgcaggtacg cgaataccca atgtatcaga
    27601 ggtgtacaaa gtatcgtact tgatttgcgc ggcatcatta tagtctacgg ctcgaaccat
    27661 actggatatg cgatccgtac ccagcactgc attgcctttc acacgatagt actgtgtgaa
    27721 gttttgtgaa ctcaaccctg aaatggttga cggtttgttg gttgccagaa gcacccaatc
    27781 gttagttaca gtagcgccgg cattgctgat cgaatcagta actgtaaaac tgattgcatc
    27841 cgcctcgccc gtattgcgca cataataacg aaccagtatg ctatcctgac cttgcaccgc
    27901 caaagctgaa tcaatcaata ccgagtcgat tacaaggaaa gccggattct gaacagtcaa
    27961 tggatctagt tggtccgtaa agaatgtaaa cttcaaggta tccaatgttg cggcggccga
    28021 gtcaaaaagt gttccgtaga gtacaattcg catgctatcg ccgcccgtcc cgtaaacacc
    28081 ggatggtaat ttgaccgtat cggcgcggaa cgtcaattgg gttgcgccgg taataccgat
    28141 ggtcttattg acattggtat ccaatgtggt gcgatacgat gagccgctgc ccgtatgtgt
    28201 aatctgaata tatgtcgagt caccgatcag gttgacgggg aatgtacctg tattgacgaa
    28261 actatatttg aaggaaacat tttgattgcg cgaaacaatt gtcgggctca acgatgaagc
    28321 aacaggttgc gcgttggcgt cgagcggtat cgtgataagt tcaaaataag tgctgtccgg
    28381 catgccgatc acgcttcccg gaatatagga aatagcagcc acgcggttgt atccgacgtt
    28441 cgttcctgtc tgatacacag caatcgcttg accgttgatg tcggtaagta ccggtattgt
    28501 actggtcaat tgaccgccat cggatgaatc aataacaacg aaggtcacta atgtatcctt
    28561 caccggatta tcatatacgt caagaagggt tgccctcagc gtacggc
//
LOCUS       DAOQGF010000036        28184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_758_length_28184_cov_7.091827, whole genome shotgun sequence.
ACCESSION   DAOQGF010000036 DAOQGF010000000
VERSION     DAOQGF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28184
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_28184_cov_7.091827"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK937_06975"
     CDS             <1..271
                     /locus_tag="PK937_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32349.1"
                     /translation="GHWELMGKAISKHWNGDIGVLSIRATDERIPLSDEDRSKVTYLI
                     DYWFMYLYYAGVSKILILLSVIVMVGFVIVAGWNVRRQLQKVEIQ"
     gene            268..987
                     /locus_tag="PK937_06980"
     CDS             268..987
                     /locus_tag="PK937_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH32350.1"
                     /translation="MNRLLVIIPAYNEASNITNVVLRVQKATDTLSNIRATVLVVNDG
                     SKDNTSDCARATGAVVLDLPYNLGIGGAVQCGFRYARDKKFDMAIQIDGDGQHDPSFI
                     HKLLSPLQEGAADMVIGSRFLNREGFQSTWLRRVGIRIFEGVNSAIIGQRITDNTSGF
                     RAYNKRAIALLAEEYPSDYPEPEAVIVLGLRGFKICETPVVMRERQGGISSIGSMSSV
                     YYMAKVLLAIGVNVFKKQNQR"
     gene            1010..1405
                     /locus_tag="PK937_06985"
     CDS             1010..1405
                     /locus_tag="PK937_06985"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNH32351.1"
                     /translation="MPELNTGVEIFRIQIIAVAGSLFIIGFIIELIRRKKLQERYALL
                     WLVAGIVMIMLSVWRDGLDKLAHVLGVGYAPALLFLVALGFGLLMMIHFSVVISSLTE
                     RNKILAQEIALLKAELNKINDESKNESGN"
     gene            1415..2032
                     /locus_tag="PK937_06990"
     CDS             1415..2032
                     /locus_tag="PK937_06990"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH32352.1"
                     /translation="MSKNLQTGTVNTPDFWDHIYQNERPRWDIGHATPVFAEWLAHRN
                     QLGTGSIAVLGCGTGHDAALFSQHGFDVTAVDFAESPLQSLQKNYPGIRTLQKDIFEL
                     NESHNEVFDFVLEYTCFCAIDPMRRDEYVQIVHTILKPGGYLIGLFFPLDERPDGPPF
                     AVRYDDVDRHFSSTFISLLDEIPEHSVPKRREKERFVIYQKKHQS"
     gene            2080..2862
                     /locus_tag="PK937_06995"
     CDS             2080..2862
                     /locus_tag="PK937_06995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNH32353.1"
                     /translation="MANAKDNLKRYTRLRNLTATLETFIFGGYRMLAKHNIDAASHGV
                     SISECTYSPWKINNEFNDLYEKIKNYTLVDKRKLYELWSLSRQLKFVRGHYIEIGVWR
                     GGSGVLLGKTLMDHGAGEKIYLCDTYEGMPAITDQNDNFYKGGELADTSTEMVHQLAH
                     DVGLNNVEILKGIFPRDTASMIPSNEVFKLVHIDVDIYSSAKETMAWVWGRMPSGGMV
                     IFDDYGYSATEGVTRFVHEFMNERNDALFIFNLLGHGIVIKR"
     gene            2929..4449
                     /gene="lysS"
                     /locus_tag="PK937_07000"
     CDS             2929..4449
                     /gene="lysS"
                     /locus_tag="PK937_07000"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNH32354.1"
                     /translation="MEEELLRVRREKLDKLREAGINPYPYKYERDAMSQELTENFEAL
                     GVKDDTQLDNPDVRRIRVCGRIMSLRSKGKTAFAHIMDPQGKIQIYVRKDAIGEAAYE
                     AFSKLYDIGDHIGVKGVLFKTHSGEITIKAEEVVLLSKSMRPLPAGKEKINDDGTVQR
                     FSAMDDVELRYRQRYADLILRPEVRDTFEKRSRVISGIREFLDKHGYLEVETPILQPL
                     YGGASARPFVTHHNALDIKLYMRIALELYLKRLIVGGIDRVYEISKIFRNEGMDRTHN
                     PEFTMLEFYAAYQDFNDLMTFTEDMLGFVCHRVHGKDSIESMGHTVSFKPPYERLSMF
                     DAIKKYTQIDVSAMDEAALREVCKTLHIPTEKSMDRGKLIDEIFSAKVQEHLIQPVFI
                     TEQPIEISPLAKKHRSKNGVVERFELFILGSEVANAFSELNDPLDQRERFEAQMGLRA
                     RGDEEAQVLDEDFLRALEYGMPPTAGEGIGIDRLVMIMTGQDSIRDVLFFPTMRPE"
     gene            4453..5268
                     /locus_tag="PK937_07005"
     CDS             4453..5268
                     /locus_tag="PK937_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32355.1"
                     /translation="MFGYIRNIILTIGSYTLLDKLGKKTISFFTGLGESAILVFQAAR
                     NLRYVFKDRHLIVEQMNLVGVNSLPLVCVTAIFAGMTSAVQAGYQLQGVASPNLLLGS
                     ATSRAITTELGPVLTGLVLSGRFAASIAAEIGTMKVTEQIDALEMLAINPIRYLALPR
                     ILAGLLMMPVLIIFTDIVAICGSMFIGFTTMDVSPQAFLSSMQKFMTMTDLFSGLIKA
                     VFFGASTAIVGCFVGFATEGGAEGVGMATIKAFVLSSAAILVNDYLVASVLLL"
     gene            5284..6045
                     /locus_tag="PK937_07010"
     CDS             5284..6045
                     /locus_tag="PK937_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH32356.1"
                     /translation="MIQVFDLYKYFRDKKILSGVNLEIESGKVTTIIGRSGCGKSVLL
                     KHIVGLLTPDRGRVLIENEEIGKLTRDELSEVRKKMGLVFQNGALFDSMTVEENITLG
                     LYEHTKMSKQEMSVKAAKLLDMVGLPGIEKYKPSELSGGMRKRVSLARALAMDPKYVL
                     YDEPTTGLDPIMSAIIDDLIVRLNEELKITSIVVTHDMRSVYTISDRVAMLHYGQVRF
                     NGTIDQLKSTNDPVVQQFIEGRIEGPIQPVLKDGI"
     gene            complement(6046..6789)
                     /locus_tag="PK937_07015"
     CDS             complement(6046..6789)
                     /locus_tag="PK937_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNH32357.1"
                     /translation="MRVSLKFLLGLFIVANTTQSQTRNVSYSLPYDTARGGLSINYDW
                     MQEIEPDVATITLSVDAQRSDLRSASEQNKLLADKILDALKNYPCSLRFDPAHTLSVP
                     STNAYSGYSNSITEPDYVVSRTAVIMTPIITSYPDIMRALSQINGMTINRIDFSTSKM
                     DSLGAEALKNAQTVLRKKAEEAGKTLGFKPGKLYSFSYAAPQLEPEYAVLSYPISSPS
                     QIQPMSNQMPHPGKLLFKITLSGMFEITP"
     gene            6887..8005
                     /gene="apbC"
                     /locus_tag="PK937_07020"
     CDS             6887..8005
                     /gene="apbC"
                     /locus_tag="PK937_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNH32358.1"
                     /translation="MITEELIFQALKSVQDPDLHKDIVSLGFVKDIVIDGGKVAFKIE
                     LTTPACPVKDLLKQQSHDAVAKISGVTSVEIIMTSRIPANKGSMNQSNLLPGVKNVIA
                     VASGKGGVGKSTVATNLAVALGMLGAKVGLLDADIYGPSIPLMMGTSEKPGSTDGQRM
                     DPILHHGIQMMSIGFLMPEEQALVWRGPMVAQALTQLMRDVAWGELDYLIVDMPPGTG
                     DAQLTLSQVVHLVGAVIVTTPQDVALLDAKRGVGMFQKVNVPILGIIENMSYYVCPNC
                     NHRTDIFSHGGAQKAAEKYEVPFLGEVPLDIETRMAGDAGTPIVLAKPDSVNAKAFMQ
                     VARNLASMVSIIHYGTAEDRSKALGQKTFFANTRLKVI"
     gene            8090..8172
                     /locus_tag="PK937_07025"
     tRNA            8090..8172
                     /locus_tag="PK937_07025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8123..8125,aa:Ser,seq:tga)
     gene            8210..8300
                     /locus_tag="PK937_07030"
     tRNA            8210..8300
                     /locus_tag="PK937_07030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8244..8246,aa:Ser,seq:gct)
     gene            8629..8910
                     /locus_tag="PK937_07035"
     CDS             8629..8910
                     /locus_tag="PK937_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32359.1"
                     /translation="MSNLFFDSNYIIIEYPLQKKKIQILKKDIVEITTIKNTERDMKK
                     DGGVSIKTKTNEYRIYWMFHKEIKLIKRKLEEIRPIQPYRGIWSEILKK"
     gene            8907..9314
                     /locus_tag="PK937_07040"
     CDS             8907..9314
                     /locus_tag="PK937_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32360.1"
                     /translation="MIKIASFLITIGLFFVSLVIFGIYVVGTHSMDYNIEALYGVFII
                     MNFMMTVFGLNTFLFKTLTSLQAEKLTLLLKFTLPISVFSFIWYIVDSINGALESDII
                     YIGNIEILFWLLLLAALIFLTLKIFNWKEKNEQ"
     gene            9311..10027
                     /locus_tag="PK937_07045"
     CDS             9311..10027
                     /locus_tag="PK937_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32361.1"
                     /translation="MKYTFLFLLIIQQSDQCVGQTSKEDVARLYVAYFELYIYSSDII
                     LGNNTYKSSKNSLATSIVSPKEIKEKYVSWKYKEKFNGLEVFCVSHACRYCSPYIVGI
                     DDELNIYRMYGFGTNISDISEQIDRNYNDTTYYLDFRDQKSILSVDVKSFVKNYLRLV
                     EFGDISENLEFTSEEVDLISTTILGRITTKYYVSRRDNKFVEIDWKYAISRDGYVSLS
                     QNASRNHGVEIIDGKVIDEN"
     gene            10300..11316
                     /locus_tag="PK937_07050"
     CDS             10300..11316
                     /locus_tag="PK937_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HNH32362.1"
                     /translation="MIRKSFICYSLVFITTVMSYNSMGSPSKNPIFIDITGGVFPLEN
                     TITESLDSNVKIVINTKFYLRVFYSDYVLFMEKSSKKRMGNRKLRDYELHRVNLLSKT
                     DSIFSIRTLHDELSAYKTIKNYCYALHGKVFRSDSIQSQLNTEGWYVSNQKYSDQLLD
                     EIDWIEIDHAKNQVKKINDSFASFKNILKKFDNTLAPLPRIYVLKLDSSNFAVSNMRI
                     WLKEDSLPALFKKPQIFNLIYDDHFGDKPAKQYYVDGLDVICYHDKVIGVTTVNPKFA
                     TQNNIHVGMSLKELLKKASPVDEEKIPSNCVIRLSCTNEWGYLSYFFILLINEKISMM
                     LLES"
     gene            11805..12317
                     /locus_tag="PK937_07055"
     CDS             11805..12317
                     /locus_tag="PK937_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH32363.1"
                     /translation="MPIRSTLFRMMILAISSLSVGIAFNQWHSSGFAWDKLIRRDHSK
                     TYMRMPRQDASKLQRLSLDEAYTLFKTKAGLFVDARQEAIFKLAHIQGAVSCYYSRAK
                     ELPIVNEWEKNKTLITYCGGPRCDQADRLAMQLIEMGFTNVFVFTGGMEEWRAAGYPI
                     EEQKRSTHPE"
     gene            12328..12795
                     /locus_tag="PK937_07060"
     CDS             12328..12795
                     /locus_tag="PK937_07060"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HNH32364.1"
                     /translation="MAWKSHIKTALRLILGIVFCYGALEKITSPYAFAAAIDAYRILP
                     AVVTPISAVWLAWLELFIGIALMVGYWTRAAAGWAILLLSIFIFAITSALLRDLVIPC
                     GCFDVNDPDEVISWKKIAEDGVLLMIAVYVFISPYARWSLSKEERLSYDGKLS"
     gene            complement(12769..16956)
                     /locus_tag="PK937_07065"
     CDS             complement(12769..16956)
                     /locus_tag="PK937_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH32365.1"
                     /translation="MRHILIAILFFHTVVIFSARAQNASHPSSLIVKFRPKSQLSKTI
                     EDRKAWIQRIQTGTKEPIHIGSAFNNKHTFFDDTYIIQLAPNLTSDEINSWIANLSAQ
                     SDIEYVTPNHLFRLDDVGINDPLADSLYWIQRTAAKKAWALTQGRSGIIIGIIDSGIE
                     YDHPDLTAQIFRNAGEMGTDAWGNDKKSNGIDDDNNGYADDWQGWDFVDAPALAGDGD
                     ATDEDNDPSDENGHGTSVAGIAAATGNNHIGVIGMAFGCRVMNLRAGGKSGFLEEDDV
                     ARAIVYAADNGAQIINCSFGDREYGPLLRDAIRYASDRNIVIVASAGNSSSSALHYPS
                     SFDDVIGVSATAKDDFFAGFSNYGTRIDVAAPGVDILTTATNHGYRFFSGTSASAPLV
                     SGLAALMLSLDSTLSRDDVRATLQQSAEDIGEIGWEPYYGHGRIHAQRALLSIGGTGV
                     VIHYPAQNQGIIGNNIPVRITAAGRFMASVRLLIGSGTNPSSWTEVASSENHAFVNDV
                     LTDLDISDYSPGTYSLRLEVTNKDGSLNETVNRFFIDHTAPVISDFVVRPMLYGKYTG
                     YHIQFQTDDLCESYVRLRIAGSSESGFPLPIKTLSQNHSIFISTSDFRGQYEAAIEVI
                     NAAGDTAYYDNNGSYYPLNLTQTELPVSAFQIDTSFKMPAAYLYPRSTDMDNDGKPEL
                     TVNTYNNAGEFAWLRTYEFSGTSGHPVSLVHDYISNGVPRDIDDSASGGKMRLLAGMG
                     SSTVVYESVNATGLPISRTYKDSNDFWGSRFADMDGDGLPELIGKKGSAYQIRKNTAS
                     SWTTWQTLPNPTGGGNLVGVPGSAVGDFDHDGHQEILLGDYDGNIFIYELTGNSATLI
                     WTDSLHGVDANAFMESGDFDGDGFPEFAVGVHSEDPSSESDAVNSYWLWRIYKTTRNN
                     DYTIAWEQYFYGYAPLAQFPSGASSQDINNDQRPDLLLSLCPYAYILTYDASSQQYVW
                     YWHSDHVLSNTIAAADANHDGFSELYIGDGTATRRFVLQGLTAYELPSALAIFPSDTD
                     RIQIQWQTRPGIDSLLIFRGTHADTLALIVTIAANAETWTDSAVQKNKPYYYRIQARY
                     GNASSDVSGLFFVRPNPKPSVVSVEMYNANFIRARFSEPIHNESLSQNFHYLLDDRYY
                     PASAIPVEGGQAVMLHFTQLASGTHRLFFYSQHDTDGTPSDTATRTFLYDGVTQRTPL
                     YIVQATYEGRNRIRLIFNKSVDIATATRTSTYRMNYDFSIVSASIVPLSSNTEVFLDV
                     DGKKYLGPIGLAFTIDAHGVKDSEGYPIDSVFGYRAGLLFSVEKLDDPFVYPNPHRAD
                     IDPPYITFAGIPDKTVVRIYSVDGRFIKQIAESGNDGGVRWDLKDKNGKRISSGVYIY
                     EAVVSGKKKLGKFAVVR"
     gene            17067..17744
                     /locus_tag="PK937_07070"
     CDS             17067..17744
                     /locus_tag="PK937_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32366.1"
                     /translation="MHFEYSDLVTIGLLVLLEGLLSADNAIVLAVQVLPLPPDEQKKA
                     LRYGIIGAFVFRIIAVLLASYLINMPGVKIIGGIYLCWLALDHFRKHGEDQTQRTPKA
                     KQFFGLSAFWSTVIAVELTDIVFSVDSILAAVALSNKQWVVVTGGIIGIITMRMVAGV
                     FLQVIKKYPRLVDGAYIIVFLIGAKLFAEYFNIHVSKIVLFASIFLIMIISIMWSNWD
                     EKKKTNV"
     gene            17787..19538
                     /locus_tag="PK937_07075"
     CDS             17787..19538
                     /locus_tag="PK937_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH32367.1"
                     /translation="MSLLKLKNSRVYALVAWIAGFLISCGTSDLNNRGIVARIGNADS
                     ITAEELNNEIIKQRIVQSGKPVFIDKQRILLDMIELKIISLYSRQSQFSVPLNKVETE
                     SAVWTHVRGSVNQRFLENNFIDSAAVERYHAYSDEVLAVQAIVLYFNSNASSSDIKSQ
                     QNVKRRIDSIYSVCTSENFNELAKKHSEYAFQGVRNPLHPVKIAYKDMTFVMENAIFS
                     VAPGKPSKPVAIGDGYAIFRLNSELKNKLQNKTKLSPNKFYQMKSDLIEKIKKDDASL
                     YVELATSNINKEWINSNIRIDEKLIGEALREYRNSNASKTVPSFAAVAWVNENPIYTF
                     AQILEKVIIDRAPDKISDSLLISTTREEIRRYLYKKIYIEEFGVNEAYFEYLTKNIEL
                     EKSKRKLEKSIDDSLTSKMTDQILKSYYEKNREKFARHLDVGFSEIKCVSFEVASAAK
                     SLAVSGVVFEQIPEVVLKKNPHWLGKIKWVSNANLLNKPQCIRDRKLIFGGINDVSEV
                     FENEDGTYSIVVVRRRNTLTSYKSYGDAGREVFGLYKAEKKEKIIQSIILDIAKKHPI
                     SIYTQNMQSIGWSKDEH"
     gene            19528..20343
                     /locus_tag="PK937_07080"
     CDS             19528..20343
                     /locus_tag="PK937_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32368.1"
                     /translation="MNIKLFTFILILGFFLSCSGDTDYLARVNQAKLKEHDMDALHSS
                     VQLSPAIREKLIQDWVDQEALYHAALKAGIDKDPVFVTQMEKLRREILVQKFIDNDLE
                     QSVTISTSEIERYYSEHPGEFIYPDDHIRVQYFWTKNRNKIRDISVKFATMSRLRKRD
                     FMEIIAPTADDSDRVGTTPYLTRDKFDERVSKQLFAKTAIDEIIGPVQLSNGYFALWY
                     VIENLPKNSQVPLSEVADDIEARLRTLKRKARTAELVEKARKEAEIEYGERKP"
     gene            complement(20404..22845)
                     /locus_tag="PK937_07085"
     CDS             complement(20404..22845)
                     /locus_tag="PK937_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNH32369.1"
                     /translation="MHRNSWIRMLMHTWLMTLPMQFGLDVSAQLAESVSKKTHRSGGT
                     TFQPNAKTTLNVPKAESALIIDGEIEDTWLQGASFGNFVENGPVEGRQPLVETQGYVL
                     FDNDYVYFAFIAYDPEMHKLRANLSDRDRMFRDDFVGVNIDPFGAQTNGFEFFVNPLG
                     IQGDLSVDVNGNEDESFDAVWESAAKIYDDRWTVEIAIPFKSLRFPDRQDQNWLIHLW
                     RVYPRENRFLYSWIPIYRGKGNQYAQAGQIAMNMEKRPGKPIEFLPYALGSRDRFVKF
                     NGDSTKGIWSKSKYDKNFGFNLKYGVTSDMTLDLAYNPDFSQIEADATQISVNNAFAL
                     YYNERRPFFLEGRDVFFVDNDLNLMYTRSINDPLLLSKLSGKTDRLSYGYLFGMDENP
                     VYIVPQEESSSEPILTTKNAINNTLRAKYELAKGTYVGILGTDRRTSDGSNTVGTIDT
                     KIRLNDTYTLSGIVSASTTDEPDATSLYGSTDRFVAFGDSLTYGFNNEKFNGIATRVT
                     LDRTARHWGFFAWFNDRSPGFRSEQGFVTNNNVREVGAFNRYTFYMDDDHPLLTRVEP
                     RVQFNRKYNYDGKLKDWWINPQVFLQFKKQTYFWFSVAALNNENFAGKQFNNIHRISF
                     ESGTDAFQKLSGGVYGEVGSYINRSGNTEDIFNPLSKVKGTKLTVWATIRPISRLRND
                     FEFNSASFRKDFGGDLIDQQLIYRNTLQYQFTKRLFLRLIGEMGTINSHRAKRDKDTD
                     EIVLDVSNQQILTKTHEIFYAISPLMSYKINPFTVFYLGANFMGRNNLDFPNYDGANL
                     DRTNFFLKFQYLMQI"
     gene            complement(22927..23517)
                     /locus_tag="PK937_07090"
     CDS             complement(22927..23517)
                     /locus_tag="PK937_07090"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNH32370.1"
                     /translation="MPHPLEDLNTLRTLIRHVYEDHKKRVLPAGQNTQAAVLIPLFLK
                     EQKIHVLLTKRTESVRTHKGQIAFPGGARDAEDTDLQITALRETEEEIGIPGKAVEIL
                     AETSPMVTPTQFHVTPFIGIVPYPFDWKINPYETESIIEIPLSHLLDERHHRKGFRMW
                     QDRTYEIHYFDYQEHTVWGVTGFILYEFLEELRPHL"
     gene            complement(23501..25504)
                     /locus_tag="PK937_07095"
     CDS             complement(23501..25504)
                     /locus_tag="PK937_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNH32371.1"
                     /translation="MQKPAPPEPIRLDRETCGDYYYATQKEWLETNGLGGFASSTIIG
                     AHTRRYHGLLCASLRPPVQRFVFLSKYDEQVRIGDKLYDLATNQYPFIIHPDGHKRMA
                     SFEYDLYPRMIYDLDGVLLEKAILMPHGSNTVIVWYHLRESSQPVTLILRPLCAFRDY
                     HSLSRETSQIRKEAEFSEHRLTIRPYDHLPPLYITAPGAFFDPKFYWYNNTEYRKEQE
                     RGLEFREDLFTHGTLSVTLEPGSALDVAASLENTRFTSETIWEMWTNEVHRRIKILSD
                     FPLKDPLARRLALAADQFMVTGQSGKTIIAGYHWFTDWGRDTMISLPGLTLATGKTED
                     AKAIISTFLPFVSQGMIPNRFPDAGEAPEYNNVDGTLWFVMACYQYYEKTKDTEFLKH
                     RLFDTLKDIIAAHTQGTRYNIHMDDDGLLYAGDSGVQLTWMDAKIGDWVVTPRIGKPV
                     EINALWYNALRIMAYFSNQLNHDDTAKAYEAMADRVKNIFESLYWNEERRCLYDVVNS
                     SGHDGSIRPNQIFAVSLPFPLLSDEKALAVINCVDAQLRTPAGLRSLASSENGYTGVY
                     AGDAYHRDAAYHQGTAWLWLIGPYMEAYLRVHHFSEEARTHIRTILQTIEPLIHDAGL
                     GTLSEIADGDAPHLPKGCIAQAWSVAEVLRIMHMLESYDAPSA"
     gene            25626..26963
                     /locus_tag="PK937_07100"
     CDS             25626..26963
                     /locus_tag="PK937_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH32372.1"
                     /translation="MFKGSVLIVDDEDNLRQLMARMLELEDFGVIQSKDKASAKTVLE
                     HEPVDVMLLDVKLPDGFAIDWISELKSEFPCMEIVLLTAYGTIPDGVKAIKSGAYEYI
                     TKGTDNDQILPIVSRASEKARLNRRIQFLQSSAEEKHAFETVIGTSPAIRECINIARR
                     VAETDTTVLLSGETGTGKEIFAQAIHYGSPRKNKPFVAVNCSAFSRELLESELFGYKA
                     GAFTGAHRNKRGLFEEADQGTLFLDEIGELSAEAQAKLLRVIELGEFIKTGDTRSTRV
                     NVRIIAATHRDLAAESAAGRFRQDLFYRISVFQVKLPALRDRSEDIPAFVEYFVRRFS
                     EKLNKRLRFADKGDWLALQTYDWPGNIRELRNVIERAVILSDGEELYSAQLLRFIYDN
                     PSSDSNTMDLKHVEREHIRKILQKTGGHKADAARLLGIGLTTLYRKIEEYALN"
     gene            27189..>28184
                     /locus_tag="PK937_07105"
     CDS             27189..>28184
                     /locus_tag="PK937_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNH32373.1"
                     /translation="MQSGNRGFKAFALLTLSTVGVVYGDIGTSPLYALREVFLGHHPI
                     VTSVENVLGALSLFFWSLTIVVALKYHILVLRIDNQGEGGVFALLAIIKQAKLQGRLT
                     MIVSGGLILGACLLYADGLITPAISVLSALEGIEVATPALSSWVIPLTIAILIGLFLV
                     QKHGTTGVGRFFGPIMVVWFVSLIVIAWPYVWSKPEVFNAVNPMYAIHFLAQQGIGSS
                     MFILGSVFLCVTGGEALYADLGHFGKHAIDTAWFILVYPALVFNYFGQGAYLLSHDVA
                     VGSNLFYLLPSETFLIPMVILATMATIIASQAMISGAFSLTQQAIGLGFFPRLKIV"
BASE COUNT         7842 a         5675 c         6726 g         7941 t
ORIGIN      
        1 cggtcattgg gaactcatgg gcaaggctat cagtaaacac tggaacgggg atattggtgt
       61 gttgtccatt cgcgccacgg atgagcgcat tccgttatcg gatgaggatc gttccaaagt
      121 aacgtatctc attgactatt ggttcatgta tctctattat gccggagttt cgaaaatctt
      181 aattctattg tctgtgatag taatggttgg gttcgtcata gtagcgggtt ggaatgttcg
      241 gcgacaatta caaaaagtgg aaatccagtg aatcgtcttt tggtgattat tcctgcatat
      301 aatgaagctt cgaacataac gaatgtcgtg cttcgagttc aaaaagctac ggatacgttg
      361 tcgaatattc gtgcgacagt gctcgtagtt aatgacggat caaaggacaa cacttccgat
      421 tgtgctcgtg caaccggcgc ggttgttttg gatttgccat ataatctagg tattggcggt
      481 gcagtacaat gcgggtttcg atatgcacgc gacaaaaaat ttgatatggc tatacagatt
      541 gacggggacg gtcaacatga cccgtccttt attcataaat tgttaagccc tttgcaagaa
      601 ggtgcggctg atatggtgat cggctcacgt tttttgaacc gagaaggatt ccagtccaca
      661 tggcttcgtc gtgtcgggat tcgtattttt gagggagtta attcggcaat tatcggtcaa
      721 cgtatcacag ataatacatc cggttttcgc gcgtataata aacgggctat cgcgttgctt
      781 gccgaggaat atccatccga ttatccggaa ccggaagccg tcatcgtatt gggtttgcgc
      841 ggatttaaaa tttgcgaaac gccggtcgtc atgcgtgaac ggcaaggtgg tatatcttca
      901 ataggttcga tgtcttcggt ttattatatg gctaaagtat tactggctat aggtgtcaac
      961 gtttttaaaa aacaaaacca acgttaggtg cgaatcacga ggtatagata tgcctgaatt
     1021 aaataccggg gttgaaattt ttagaattca gattatcgca gtggcgggta gtctttttat
     1081 cattggtttt ataatagaat tgatacgtag aaaaaagctg caagaaaggt acgcgcttct
     1141 gtggttagtc gccgggatag ttatgatcat gttgtcggta tggcgcgatg ggttggataa
     1201 attggcccac gttttgggtg ttggatacgc tccggcgtta ttattcttag tggcgcttgg
     1261 tttcgggtta cttatgatga ttcatttttc ggtagttatt tcttcgctaa ctgaacgcaa
     1321 taaaatactc gcgcaagaga ttgctttgtt gaaggctgag ttaaacaaaa tcaatgatga
     1381 gtctaaaaat gagagtggaa attaatctaa tctgatgtca aagaatttac agactggtac
     1441 ggtcaatacc cctgattttt gggatcatat ctatcaaaat gagcgcccgc gttgggatat
     1501 cgggcatgca acgccggtgt ttgcggaatg gcttgcgcat cgcaatcaac ttggcacggg
     1561 atccatcgcg gtactgggtt gcggtacagg gcatgatgcg gcgctttttt cgcagcatgg
     1621 ttttgatgtg acggcagttg attttgccga atcgccgttg caatcgcttc agaaaaatta
     1681 tcccggcatt cgtacattgc aaaaagatat ttttgagtta aatgagtcgc acaacgaagt
     1741 gtttgatttt gttttggaat acacatgctt ctgtgcgatt gatccgatgc gacgggacga
     1801 atacgtgcag attgtccata cgattctcaa accgggaggg tacctgatcg gtttgttttt
     1861 tccgttggac gaaagaccgg acgggccacc gtttgccgta cggtatgacg atgtggacag
     1921 gcatttttca tcgacgttta tatcattgct ggatgaaata ccggagcact cggttcccaa
     1981 gcgaagggaa aaagaacgat ttgtaattta tcaaaaaaag catcaaagtt agaagaatta
     2041 atatttaact tcctcagtcc aaaaaggagt ataaaagaaa tggccaacgc gaaggataac
     2101 ttaaaacgtt atacacgtct gcggaatttg actgcaacat tagaaacttt tattttcggt
     2161 ggttaccgta tgctcgcaaa gcataatatt gatgcggctt cccacggtgt aagtatttcc
     2221 gagtgcactt atagcccttg gaaaatcaat aatgaattta atgatcttta cgaaaaaata
     2281 aaaaattata ctttggtaga taaaagaaaa ctttatgaac tttggagttt gtctcgtcaa
     2341 ttaaagttcg ttcggggaca ctatattgaa atcggtgtgt ggcgaggggg gtcgggcgta
     2401 ctcttgggaa aaacacttat ggatcatgga gcgggcgaga aaatctattt atgcgacaca
     2461 tatgaaggaa tgccggccat tacggatcaa aatgataact tttacaaagg aggagaattg
     2521 gccgatacct cgactgaaat ggttcatcaa ttggcgcacg atgtaggtct taacaatgtt
     2581 gaaattttga agggcatctt tccccgtgat acagcatcta tgattccgtc gaatgaagta
     2641 ttcaaattgg tacatattga tgttgatatc tattcgtccg ctaaggaaac gatggcatgg
     2701 gtatggggac gaatgccgtc tggtgggatg gtgatttttg atgactatgg gtattccgcg
     2761 actgaaggcg taacgcggtt tgttcacgaa tttatgaatg aaagaaatga tgcattgttt
     2821 atatttaacc ttttaggtca tggcattgta attaagcgtt gaggtgaaga ggtaccattt
     2881 aatactatag gtaccattta atactataga taaaggtaag agggcattgt ggaagaagaa
     2941 ttattacgtg tacgacggga aaaattagac aagcttcgtg aagccggtat caatccgtat
     3001 ccttacaaat atgaacggga cgcgatgtcg caggagttga cggaaaactt tgaagcgctc
     3061 ggcgtcaaag atgacacgca attagacaat ccggatgtac gtcggatacg cgtatgcggt
     3121 cgtatcatgt cgttgcgcag caaaggcaaa acagcgtttg cgcatattat ggacccgcag
     3181 gggaaaattc agatctatgt acgtaaagac gctatcggtg aagccgcata tgaagcgttt
     3241 agtaaactgt atgatatcgg cgatcatatc ggtgtcaaag gcgtactttt taagacacac
     3301 tccggcgaga tcaccatcaa agcggaagaa gtcgtattgc tttccaagag catgcgtccg
     3361 cttccggccg gaaaagaaaa gatcaacgac gacggaacgg tacagcgttt ttccgcgatg
     3421 gatgatgtgg agttgagata ccgccaacgt tatgccgatc tgatcctgag acccgaagtg
     3481 cgggatactt ttgaaaaaag atcgcgcgtg atttcgggta ttcgcgaatt tttggataaa
     3541 cacggttatc tggaagtcga aacgccgatt ttacagccgt tgtatggcgg tgcatccgcc
     3601 aggccttttg tgacgcacca caatgcgctc gacatcaagt tgtatatgcg tattgcgttg
     3661 gagctgtacc tcaaacgact catcgtcgga ggtattgacc gcgtgtacga aatttccaag
     3721 atctttcgta atgaaggtat ggatcgcacg cataatcccg aatttacgat gctcgagttt
     3781 tatgctgcgt atcaggattt taatgatctc atgacattta ccgaagacat gcttggtttt
     3841 gtctgccatc gggtacatgg taaagacagc atcgaatcca tggggcatac ggtatcgttc
     3901 aaaccgcctt acgagcggct ctctatgttt gatgcgatca aaaaatatac acagatcgat
     3961 gtatcggcta tggatgaagc cgcgttgcgt gaagtttgca aaacgctgca tattcctacc
     4021 gaaaaatcca tggatcgggg aaaattgatt gacgaaattt ttagcgcaaa agtgcaggag
     4081 catctgatcc aaccggtgtt tatcaccgaa cagccgattg agatttctcc actggccaaa
     4141 aaacaccgtt ccaaaaatgg cgtggtcgag cggtttgaat tatttatcct cggcagtgaa
     4201 gtggccaacg ccttcagcga gctgaatgat ccactggatc agcgtgaacg cttcgaagcg
     4261 cagatgggtt tgcgtgcgcg gggcgacgaa gaagcgcagg ttttggatga agattttctg
     4321 cgggctttgg aatacggcat gccgccgaca gcgggggaag gtatcggtat agatcgcctt
     4381 gtcatgatca tgacgggtca ggattccata cgcgatgtgt tattttttcc tacgatgcga
     4441 ccggaataaa ctatgtttgg atatattcga aatattattc tcacgatcgg cagttatacg
     4501 ctgctcgaca aacttggaaa aaagaccatt tcttttttca cgggtttggg cgaatcggcc
     4561 attctcgttt ttcaggctgc acgcaatttg cggtatgttt tcaaagatcg tcatttgatc
     4621 gtcgagcaga tgaatctggt cggcgttaat tcactgccgc tggtatgtgt tacggcgatt
     4681 tttgcaggta tgacatctgc cgtccaagcc ggttatcagc ttcagggtgt agcttcacct
     4741 aatctgttgc tgggttcagc aacgtcacgt gcgatcacga cggagcttgg accggtgctt
     4801 accggtttgg ttttgtcggg gcgatttgcg gcatccatcg ctgcggagat cggtacgatg
     4861 aaggtgacgg agcaaataga tgcgctggag atgcttgcaa tcaatccgat tcgttatctg
     4921 gcattgcctc gtattttggc ggggcttctg atgatgccgg ttctgattat ctttacggac
     4981 attgttgcga tttgcggttc gatgtttatc ggatttacga cgatggatgt ttcgccgcag
     5041 gcatttttgt ccagcatgca aaaatttatg accatgaccg atttgttttc cggtcttatc
     5101 aaagccgtat tttttggcgc ctctacagcg atcgtcggat gttttgtcgg ttttgccacg
     5161 gaaggcggag ccgaaggtgt ggggatggcc acgatcaaag cgtttgtatt atccagcgcg
     5221 gcgattctcg tcaatgatta tttggtcgcc tccgtattgc tcttgtaatt agaaaaaaca
     5281 actatgatcc aggtttttga tttatacaaa tatttccgcg ataaaaaaat tctcagcggc
     5341 gtcaacctcg aaatcgagtc cggtaaagtt acgaccatta tcgggcgcag cggttgcggt
     5401 aaaagtgttt tactcaaaca catcgtcggc ttattgacgc cggaccgcgg acgcgttctt
     5461 atcgaaaacg aagaaatagg aaagctgacg cgtgacgaac tttccgaagt gcgaaaaaaa
     5521 atggggctcg tgtttcaaaa cggcgcttta tttgattcga tgaccgtcga agaaaatatc
     5581 acactcggcc tgtatgagca taccaaaatg agcaaacagg agatgagtgt caaagccgcc
     5641 aaattgctgg atatggtcgg cttgcccggg attgaaaaat acaaaccatc cgaactctct
     5701 ggcggtatgc gaaaacgtgt gagcctggcc cgtgcgctcg ccatggatcc taaatacgtg
     5761 ctgtatgatg aaccgaccac cggtttggat cccatcatgt cggcgatcat tgatgacttg
     5821 atcgtacggc tcaatgaaga actcaagatt acttctatcg tcgtcacgca tgatatgcgc
     5881 agtgtgtaca cgatttctga tcgtgtggcg atgctacact acggtcaggt acgatttaat
     5941 ggaactatcg accaactgaa atctacaaac gatcccgtcg tccaacagtt tatcgaaggc
     6001 cgcatcgaag gtccgataca accggttttg aaagacggca tttaattaag gtgtaatctc
     6061 aaacatgccg ctgagtgtaa ttttaaataa aagtttgccg ggatgcggca tctgattgct
     6121 catgggttgt atttggctcg gtgaagaaat tggataagac aaaacggcat attccggttc
     6181 gagctgcggt gcggcgtagg agaatgaata taacttaccc ggtttgaagc ccagcgtttt
     6241 gccggcttct tcagcttttt ttcggaggac agtctgtgca tttttcaatg cttcggcacc
     6301 gagactgtcc attttggatg ttgaaaaatc aatacggtta atcgtcatac cgttgatttg
     6361 agataaggct ctcatgatgt ctggataaga ggttatgatc ggtgtcatga tgacggctgt
     6421 acggcttacg acatagtcgg gctccgttat ggagttggaa tatcctgagt aagcgttggt
     6481 ggatggtaca ctcaacgtat gcgccggatc gaaacgcaaa gaacacgggt agttttttag
     6541 cgcatccagt attttatctg ccaaaagttt gttttgttcg ctggcggaac gcaaatccga
     6601 gcgttgtgca tccacactca aggttatcgt cgcgacgtcc ggttcgattt cttgcatcca
     6661 gtcgtaattg atactcaatc caccgcgggc tgtatcatac ggtaaactgt acgaaacatt
     6721 acgtgtttga ctttgcgtcg tatttgcaac aatgaacagg ccgagcaaaa atttcaatga
     6781 gacgcgcata aggttctccc gttgaataaa aagaaaaatt tgaataaaat acatatatcg
     6841 taaataagtc aatactacat tattatgtag tcataataga ggaaccatga tcacagagga
     6901 actgattttt caagcgctca aaagcgtaca agaccccgat ttgcacaaag acatcgtatc
     6961 gctcggattt gtaaaagata tcgtcattga cggcggtaaa gtggcgttca aaatcgaatt
     7021 gaccacaccg gcttgtcccg tcaaagattt actcaaacaa caaagtcatg atgccgtggc
     7081 taagatcagc ggcgtgacct cggtggaaat catcatgacg tcgcggattc cggcgaataa
     7141 aggttccatg aatcaaagta atttattgcc cggtgttaaa aacgtcattg ccgtagcgag
     7201 cggtaaaggt ggtgtgggaa agtccaccgt cgcaaccaat ctcgctgtag cgcttggtat
     7261 gttgggtgcg aaagtcggat tattggacgc cgatatttac gggccgagta ttccgctgat
     7321 gatggggact tcggagaagc cgggttccac ggatggtcag cgtatggatc cgatcctgca
     7381 tcacggcatt cagatgatgt ccatcggatt tcttatgccg gaggagcaag cgcttgtatg
     7441 gcgcggccct atggtcgcac aggcgttgac ccagctgatg cgcgacgtgg cctggggcga
     7501 actggattat ctgatcgtgg atatgccgcc cggtacgggc gatgcgcagc ttacgctgtc
     7561 gcaggttgtt caccttgtcg gcgccgtcat cgtgacaacg ccacaggatg tcgccctctt
     7621 ggatgccaaa cgcggcgtgg ggatgttcca aaaagtcaat gtgcccattc tcggtattat
     7681 cgaaaacatg agttattatg tgtgccccaa ttgcaatcac cgtacggata ttttttcgca
     7741 tggcggagca caaaaagccg ctgaaaaata tgaagtgcct tttttaggtg aagtgccgct
     7801 agatatcgag acgcgtatgg cgggcgatgc cggtacgccg atcgttttgg cgaaaccgga
     7861 ttcggtgaat gctaaagctt ttatgcaggt tgcgcgcaat ctggcttcga tggtaagcat
     7921 tattcactac ggtacggcgg aagatcggtc gaaggcgttg gggcagaaaa ctttttttgc
     7981 caatacacgc ctcaaagtga tttaagcgaa taaatagttg actgcaatga tggtttcacc
     8041 tatattgcgt cccgttttta agacggtagg taaatggcct ttttggaatg gaggattagg
     8101 ctaattggta agtcagcagt cttgaaaact gccgtccgaa aggacttggg ggttcgagtc
     8161 cctcatcctc cgctgttctt ttggatgtga tccatatacg aatgcattgg gagaggtggc
     8221 cgagtggctt aaggcagcgg tttgctaaac cgccatacgg ttgtaaagcc gtatcgggag
     8281 ttcgaatctc cccttctccg caaaagctca gagtaatctg agcttttttt gttgagggag
     8341 tagatgtaaa atattatgtg tatgtagtat ggagtgatca actgtaaaaa agatatgtcg
     8401 gataaaatac aattcagtag acattctcag ggaaatagta tgtgtaactt tatgtttaac
     8461 aagcaaagcg taattcgctc acttatcttg ctttttttaa tttgtatagt cttaacgttt
     8521 tattatgggt atagtgatga ccagacaata aatattagag aaatgattct aaggttattg
     8581 ccaacagtat taatttttct aatttgttac gtgcggttaa gagaatcgtt gtcaaattta
     8641 ttttttgata gcaactatat tattattgag tatcctttgc aaaagaaaaa gatacaaata
     8701 ttgaaaaaag atatagttga aattactact atcaagaata cagaaagaga catgaagaaa
     8761 gatggtggtg tgagtataaa aacaaaaact aatgaatatc ggatatactg gatgtttcat
     8821 aaagagatca aattgattaa aagaaaactg gaagaaatta gaccaataca accttataga
     8881 ggaatttggt ctgagattct aaagaaatga ttaaaatagc atcattttta ataactatag
     8941 gtctcttctt tgtaagctta gttatatttg gaatatatgt cgtgggcacc catagcatgg
     9001 attataatat agaggccttg tacggtgttt ttattattat gaattttatg atgacagtgt
     9061 ttgggttaaa cactttcttg tttaaaacgc tcacatcttt acaagccgag aaacttacac
     9121 ttttacttaa attcacgtta ccaatttcag tgtttagttt tatttggtat atagttgata
     9181 gtattaacgg tgcccttgag tcagatataa tttatattgg aaatatagag atattgtttt
     9241 ggttgctttt gttagcagct cttatttttt taactctcaa aattttcaat tggaaagaga
     9301 aaaatgaaca atgaaataca cattcctctt cctcttaata atacagcaaa gtgatcaatg
     9361 tgttggacaa acatccaaag aagacgttgc gcgtttgtat gttgcttatt ttgagttata
     9421 tatctatagc tcagatatta ttctcggaaa caatacctac aagagctcaa aaaacagtct
     9481 agcaacatca attgtttctc caaaggaaat caaagaaaaa tatgtttctt ggaagtacaa
     9541 ggaaaaattt aatgggttag aagtgttttg tgtgagtcat gcatgtaggt actgtagtcc
     9601 atacatagtc ggtattgatg atgagttgaa tatctataga atgtatggtt ttgggacgaa
     9661 tataagcgac atttctgagc aaattgatcg caattacaat gatacaacgt actatcttga
     9721 ttttagagat caaaagtcaa tcttaagcgt agacgtaaaa tcatttgtaa agaattactt
     9781 acgcttggtt gaatttggag atatttctga gaacttggaa tttaccagtg aagaggttga
     9841 tttgatatcg actacaattc tcggtagaat tacaacaaag tattatgtat ctcgccgaga
     9901 caataagttt gttgaaatag attggaagta tgcgataagt cgcgatggat acgtaagttt
     9961 atctcaaaac gcatcaagaa atcatggggt cgaaattatt gatggtaaag ttatagatga
    10021 aaattaatgt gtttgtagtc acaaatatca tgtttctgcg ctatcacgtc atgcccagat
    10081 cacttaaata gcatgactac tcggcttgta gtgttgacgc acttctgcag tatatgttgg
    10141 ggttatctag gaaggtaaag gtaatgaaaa tatttttgtc gcacgtggcc cagaacaaat
    10201 ggatgtcccg caagttagcg ataatgtgaa ccttgagaga cgggacaata ctcctacatc
    10261 tgtgccgatc aatattagtg ttactaccga ggagataaaa tgatacgtaa atcattcatt
    10321 tgctattctc tggtttttat taccacagtg atgagttata actcaatggg ctcgccttca
    10381 aaaaacccta tttttatcga tattacagga ggcgtttttc cgttggagaa cacgattaca
    10441 gaatcattag attcaaatgt aaagatcgtt attaatacca agttctattt gcgagtcttt
    10501 tatagtgatt acgtgttgtt catggagaaa tcatccaaaa aaagaatggg aaatcgtaaa
    10561 ctaagggatt atgagctaca tagggttaac cttttgtcaa aaacggatag cattttttct
    10621 atccgtactt tgcatgatga actgtctgcc tataaaacta taaaaaatta ctgctatgca
    10681 ttgcatggaa aagtgtttag aagtgattcc attcaatcac agttaaatac agaaggttgg
    10741 tatgtttcta atcaaaagta cagcgaccag ttgcttgatg aaatagactg gattgaaatt
    10801 gatcatgcca agaatcaggt gaaaaagatc aatgactcat ttgcgagttt caaaaatatc
    10861 ctaaaaaaat ttgataatac cttggctccg cttccgcgta tttatgtttt aaaattggat
    10921 agttcaaatt ttgcagtttc aaacatgcgt atatggctta aagaagattc tctgccggca
    10981 ttatttaaaa aaccacaaat ttttaatcta atatatgatg atcattttgg cgataagcct
    11041 gccaaacaat actatgttga tgggttggat gtaatatgct atcatgacaa agtcatcgga
    11101 gttacaacag ttaatcccaa gtttgcaacg caaaacaaca tacacgttgg gatgtcactc
    11161 aaggaattat tgaaaaaagc aagtccagtt gatgaagaga aaataccatc taactgtgta
    11221 attcgcctta gctgtacgaa tgaatggggg tatctttcgt atttttttat ccttcttatt
    11281 aatgaaaaaa tatcaatgat gctattggag tcatgatgaa tactgcgtgt attcattata
    11341 ttggtatagg ttgacttcgt agtaatgagt ttgctaaggg gtcatttggc gactttatca
    11401 taatagttat gaattgattg aggcgtcaaa ctagtacaaa acttagttac cgttgcatgc
    11461 atgaaaactt gaggcgaggg gagagacttc tcccctaata tgatgaattg atatctgaat
    11521 gtgataaaat ggcggttgtg aaataaaatt agccaatgat cagatgctgg caagcacaat
    11581 cgtaaaccgg tatggaaaat gttacaataa aaagtagagt attaccgcat tacagcgtta
    11641 gagttacaca aaagctattt tctatcaaca catcagtgta aatcaaaacc agcctaacta
    11701 tgcaattaga aacggacgcg gtatggattg agatgacatt cttaccgtgc ttgtctttga
    11761 gttttcattg atatagatta gattaatctc aggagtgaca aactatgcct atccgttcca
    11821 ctctttttcg tatgatgatc ctcgcgattt ctagtttgtc tgtcggcatt gcatttaatc
    11881 aatggcacag cagtggtttc gcatgggata aattgatacg ccgcgaccat tcaaaaactt
    11941 atatgcgtat gccgcgtcag gatgcatcca agttgcaaag actttcgctg gacgaagcct
    12001 atacgctttt taaaaccaaa gccggattat ttgtggacgc acgccaggaa gcgattttca
    12061 aattggctca catccagggc gccgtgtcgt gttattatag tcgcgcaaag gaactaccca
    12121 tagtcaatga atgggaaaaa aataagacat tgataaccta ttgcggcgga ccgcgttgcg
    12181 atcaggccga ccgtctcgcc atgcagttga tcgaaatggg ttttaccaat gtttttgttt
    12241 ttaccggagg aatggaagaa tggcgcgctg ccggttatcc gatcgaagaa caaaaacgca
    12301 gtactcaccc tgaataaaaa aataattatg gcttggaaaa gtcatataaa aaccgcgtta
    12361 cgtctaattc tcggcatcgt attttgttat ggcgccttag aaaaaatcac atcgccatac
    12421 gcctttgccg ccgcgataga cgcctatcgt attttacccg ccgtggtgac accgatttca
    12481 gccgtctggc tagcgtggct ggagttattc atcggtatag cactcatggt cggatattgg
    12541 actcgcgcgg ccgccggatg ggccatccta cttttgagta tatttatttt tgctatcacc
    12601 tcagcgctcc tgcgcgatct ggttataccg tgcggatgct ttgatgtgaa tgatccggat
    12661 gaagtgataa gttggaaaaa aatagccgaa gatggcgttt tactgatgat cgcggtgtat
    12721 gtttttatat cgccgtatgc acggtggtcc ttatcaaaag aagagcgtct atcgtacgac
    12781 ggcaaacttt cctaactttt tctttcccga cacgaccgct tcatatatat acacgccgga
    12841 tgagattctc ttgccgtttt tatcctttaa atcccatcgt accccaccgt catttccgct
    12901 ttcggcgatc tgttttataa aacgtccgtc aacggaataa atccgtacca cggtcttatc
    12961 gggaatacct gcgaaggtga tataaggcgg atctatatcg gcgcgatggg gattgggata
    13021 cacaaacgga tcatctaatt tttcaacgct gaataataat ccggcacgat agccaaaaac
    13081 agaatctatc ggatatcctt cgctgtcctt gacgccgtgt gcgtctatcg taaaagctaa
    13141 accgatcggg cctaaatatt tttttccgtc aacgtccaga aacacttccg tattggatga
    13201 caggggaacg atacttgccg agacaattga gaaatcataa ttcatacgat acgtcgaggt
    13261 gcgtgtcgct gtagcgatgt ccacggattt attgaaaatc aaacgaatgc ggttgcgacc
    13321 ttcgtatgtc gcctgcacga tgtacaaagg tgtgcgttgt gtaactccgt catacaaaaa
    13381 cgtacgggtg gcagtgtccg atggcgtgcc atcggtatcg tgctgtgaat aaaaaaacaa
    13441 tcggtgcgta ccggatgcaa gttgcgtaaa gtgaagcatc acggcttgac cgccttcgac
    13501 aggtatcgcg ctcgccggat aataccggtc atccagcaaa taatgaaaat tctgactcag
    13561 gctttcgttg tgtatcggtt cagaaaaccg ggctcgtata aaattggcat tatacatttc
    13621 gaccgataca actgatggtt tcggattggg gcgcacaaaa aaaagaccgg atacatcgga
    13681 tgaggcgttg ccgtagcgcg cttgtattct gtaataatac ggtttgtttt tttgaacggc
    13741 tgaatcggtc cacgtttcgg cgttagccgc gatcgtcacg ataagcgcta atgtatctgc
    13801 atgcgtacct cgaaaaatga gcagtgaatc tatacccggg cgcgtttgcc attgtatttg
    13861 aatgcgatcg gtatcggatg gaaatatggc caacgcggag ggtaattcgt aagcggtcag
    13921 gccttgcaag acaaatcggc gtgtagcggt accatcgccg atgtatagtt cggaaaatcc
    13981 gtcatgattt gcatccgctg cagcgatagt attgcttagc acatgatcgc tatgccaata
    14041 ccacacatac tgctggcttg atgcatcgta agtcaggata taggcatacg gacacaacga
    14101 caataataaa tccgggcgtt gatcgttatt gatatcctgc gacgaagcgc cggatggaaa
    14161 ttgcgctaac ggcgcatacc cataaaaata ttgttcccat gcgatggtgt agtcgttatt
    14221 ccgggtagtt ttgtatatac gccataacca atagctgttg acggcatcgc tttcggaaga
    14281 aggatcttcc gagtgaacgc cgacggcgaa ttccgggaat ccgtccccgt cgaaatcacc
    14341 gctctccatg aacgcattgg catccacacc gtgcaacgaa tcggtccaaa tcagcgtcgc
    14401 cgagtttccg gttaattcgt agataaaaat atttccgtcg taatcgccga ggagaatttc
    14461 ttgatggccg tcatgatcaa aatcgccgac ggccgaaccg ggaacgccga ccagattacc
    14521 accgccggtc ggattgggga gcgtttgcca ggttgtccag gatgaagcag tgttttttcg
    14581 tatttgataa gcgcttcctt tttttccgat caattcaggt aaaccgtcgc cgtccatgtc
    14641 ggcaaaccgc gatccccaaa aatcattaga atctttatac gttcgtgaaa tcggtaaacc
    14701 ggtcgcgttc acgctttcgt acacgacggt ggatgatccc ataccggcga gcaatcgcat
    14761 tttgccgccg gaagcgctgt catctatatc gcggggtacg ccgttggaaa tataatcatg
    14821 aactaaagaa acaggatggc cgctcgttcc tgaaaattca tacgtgcgta accacgcaaa
    14881 ttctccggcg ttattatacg tattcaccgt caattccggt tttccgtcgt tatccatatc
    14941 ggtagaacgg ggatacaaat aggcggcggg cattttgaaa gaagtgtcta tttgaaatgc
    15001 cgaaaccggt aactccgttt gcgtgagatt gagcggataa taacttccgt tattatcgta
    15061 atacgcggta tctccggccg cgttgatcac ttcgatagcg gcttcgtact ggccccggaa
    15121 atcggatgtg ctgatgaaaa tactgtgatt ttgtgaaagc gttttgatgg gcaagggaaa
    15181 tcccgattcc gatgaaccgg cgatgcgcaa tcgtacataa ctttcgcaca gatcatccgt
    15241 ttggaactga atatgatacc ctgtgtattt gccatacaac atagggcgca ctacgaagtc
    15301 cgagatcacc ggcgcggtat gatcaataaa aaaacgattc accgtttcgt tcaacgagcc
    15361 gtctttgtta gtcacttcga gtctcagcga ataagttccg ggcgaataat cacttatatc
    15421 caaatcggtg agcacatcat tgacaaacgc atgattttcc gacgacgcga cttccgtcca
    15481 cgaagaaggg ttcgtgccgc taccgatcaa cagacggacg gacgccataa aacgcccggc
    15541 cgcagtaatg cggacaggga tgttatttcc gataataccc tgattttgtg cagggtaatg
    15601 aataacgacg cctgtgccgc cgatagacaa cagtgcgcgc tgggcatgga tacgaccatg
    15661 tccatagtat ggttcccaac cgatttctcc gatatcttcg gcgctttgct gtaacgtcgc
    15721 acgtacatca tcgcgggaaa gcgtgctatc cagcgacagc atcagcgcgg ccaaacccga
    15781 taccaagggg gccgaagccg aagtgcctga aaaaaaacga tacccatgat tcgtcgccgt
    15841 cgttagaata tctacgcccg gtgcggccac gtcaatacgc gtaccataat ttgagaaacc
    15901 ggcaaaaaaa tcgtctttgg cggtagcgct gacaccgatg acatcatcaa acgatgacgg
    15961 ataatgcagt gcagagctgg acgaattgcc ggcggaagcg acgatcacga tattgcgatc
    16021 gctcgcgtaa cgtatcgcat cgcgcaataa aggtccatat tcccgatctc caaagctaca
    16081 gttgatgatt tgtgcgccgt tatccgctgc gtaaacgata gcccgcgcca catcgtcttc
    16141 ttcgagaaaa cctgatttcc ctccggcgcg cagattcatg acacggcatc cgaacgccat
    16201 accgatcacg ccgatatggt tattaccggt agcggcggct atgcccgcta cggaggtgcc
    16261 atgcccgttt tcatcagaag ggtcattatc ttcatccgtg gcatcgccgt cgccagcgag
    16321 cgcaggggcg tcaacaaaat cccacccttg ccagtcgtcg gcatatccgt tgttatcatc
    16381 gtctatgccg ttggattttt tgtcgtttcc ccatgcgtcg gttcccattt caccggcgtt
    16441 acgaaagatc tgagcggtaa ggtcgggatg atcgtattcg ataccggaat ctataatccc
    16501 gataatgatg ccggagcggc cttgcgtaag agcccaggct tttttagcag cggtgcgttg
    16561 tatccaataa agactgtcgg cgagcgggtc attgatacct acatcgtcca atcgaaacag
    16621 atgattgggc gtaacgtatt caatatcaga ctgtgcggac aagttggcga tccaagagtt
    16681 tatttcatcg gatgtaaggt tcggcgcaag ttgtattata taggtatcat caaaaaaagt
    16741 atgcttattg ttgaaagcgg agccgatatg aatcggttct tttgtgccgg tttggatacg
    16801 ctgtatccat gctttgcggt cttcgattgt tttggataat tgacttttcg ggcgaaattt
    16861 tacaataagt gatgagggat gcgatgcgtt ttgggcacga gcagaaaaaa tgacaaccgt
    16921 gtgaaaaaat aatatagcaa tcaatatatg gcgcatgaaa tattatacta aaagacacgg
    16981 gacggtgcaa cgtaattgcg cttgatagtt ctatttcaca acgatatatt cctcggcttt
    17041 tatatgaaac caaatgagga tatgttgtgc attttgaata ctcagatctc gtaaccatcg
    17101 ggctgctggt cttactggaa ggtttgctta gcgccgataa cgcaattgtt ttagccgttc
    17161 aggttttgcc gttaccgccg gatgaacaaa agaaagcgtt gcggtatggt attatcggtg
    17221 cgtttgtatt tcggatcatt gccgtattat tggcatctta tctgatcaat atgccggggg
    17281 tgaagatcat cggagggata tatttgtgct ggctcgcctt ggaccacttt cgtaagcacg
    17341 gtgaagatca aacgcaacgt acacctaaag ctaaacaatt tttcggcttg tcagccttct
    17401 ggagtacggt gattgccgtt gagttgacgg atattgtttt ttccgtagat tcgattttag
    17461 ccgcggtagc tttgtccaat aagcagtggg ttgtcgttac gggtggcatt atcggaatca
    17521 tcacaatgcg aatggtcgcg ggtgtttttt tgcaagtgat aaaaaaatat ccgcgtttgg
    17581 tggacggcgc ctatatcatc gtttttctta tcggcgccaa attatttgcc gagtatttta
    17641 atatccatgt ttcgaagatt gttctctttg catccatttt tctgattatg atcatcagta
    17701 ttatgtggtc gaattgggat gagaagaaaa aaacgaatgt ttagttgtta actgagtgac
    17761 agtgttttat tgttaaggaa ttagatatga gtttattaaa attaaaaaat tcgcgcgttt
    17821 atgctttagt tgcatggatt gccggtttcc ttatttcttg cgggacatca gacctgaata
    17881 accgaggcat agtagctcgg ataggcaatg ccgatagtat aaccgccgaa gaactgaata
    17941 acgaaattat taaacaacgg attgtgcaat ccgggaaacc ggtttttatt gataaacaac
    18001 gtatactgct cgatatgatt gagttgaaga ttattagttt gtattctcgc caatctcaat
    18061 ttagtgtgcc tttaaataaa gttgaaactg aatcggctgt atggacgcat gtcagaggaa
    18121 gcgtgaacca acgcttttta gaaaataatt ttatagactc ggcggctgtt gaaagatacc
    18181 atgcatattc tgatgaagtg ttggctgtgc aagccattgt gctatatttt aattccaatg
    18241 cctccagttc agatatcaag tcacagcaaa atgtcaaacg ccgcatcgat tcgatctatt
    18301 cggtttgtac atccgaaaat tttaacgaac ttgcaaaaaa acactcagag tacgcgttcc
    18361 aaggtgttcg caatccgttg cacccagtga aaatcgcata caaggatatg acttttgtga
    18421 tggagaacgc tatttttagc gttgctcccg gtaaaccgtc aaagccggtt gctattggtg
    18481 atggatatgc gatttttcga cttaactccg aattaaaaaa caaactacaa aacaaaacaa
    18541 aattatcgcc gaataaattt tatcaaatga agagcgatct tatcgaaaaa attaagaagg
    18601 atgacgcttc attatatgta gaattagcta cttcgaatat taataaggaa tggattaatt
    18661 ctaatattcg tattgacgaa aaattaattg gagaagcgtt gcgtgaatat aggaattcaa
    18721 atgcttcaaa gacggtaccg tctttcgcag cagtcgcttg ggttaacgaa aatccgatct
    18781 atacattcgc tcaaattttg gaaaaagtga taattgatcg agcacctgat aagatttcag
    18841 atagtttatt gatttctaca acgcgtgaag aaattaggag gtatttatat aaaaaaatat
    18901 atatagaaga gttcggagtt aatgaagcat attttgagta cttaacaaaa aatatcgaac
    18961 ttgaaaagtc aaaacgcaaa cttgagaaat cgatagatga ttcgttgaca agcaagatga
    19021 cggatcaaat tttgaagagt tattatgaaa agaatcgcga gaagtttgcg cggcacttag
    19081 atgtagggtt ttccgaaatc aagtgcgtta gttttgaggt tgcgtcggcc gctaaatctt
    19141 tagccgtatc gggtgttgtg tttgaacaaa tacccgaagt cgtgttaaag aagaacccgc
    19201 attggttagg aaaaatcaag tgggtatcaa acgcaaatct tctgaacaag ccacaatgca
    19261 ttcgcgatcg aaagttgatt tttggcggca ttaacgatgt aagtgaagtt tttgaaaatg
    19321 aagacggtac gtattcgatt gttgttgtgc gtcggcgtaa tactctaaca agttataaat
    19381 cgtacggcga tgcgggtcgc gaagttttcg gattgtataa ggccgagaaa aaagaaaaaa
    19441 ttattcaaag tattatttta gatatagcca aaaagcatcc gatcagtatt tatacacaga
    19501 acatgcagag tattgggtgg agcaaagatg aacattaagt tattcacctt tattttaata
    19561 ctggggtttt ttttatcctg ttccggtgac acggattacc ttgcgcgcgt caatcaggcg
    19621 aaattgaaag agcatgatat ggacgccttg cattcgtcag tacagttatc gccggcgata
    19681 cgtgaaaagt taattcagga ctgggtggat caggaagcgc tttatcatgc ggcgttgaaa
    19741 gcgggtattg ataaagatcc ggttttcgta acgcaaatgg aaaagttgcg acgtgaaatc
    19801 ctcgttcaaa aatttataga taacgattta gaacaaagtg taaccatttc gacgtcggaa
    19861 attgaacgtt attatagtga gcatcccgga gaatttatct atccggatga tcatattcgg
    19921 gtgcaatatt tctggacaaa aaatcgaaat aaaatccgcg atatcagtgt aaagtttgcc
    19981 acgatgtcgc gccttcgtaa acgcgatttt atggaaatta ttgcgccgac ggctgatgac
    20041 agcgatcgtg tcggtacaac accctatctg acgcgcgata agtttgatga acgcgtcagc
    20101 aaacaacttt ttgccaaaac cgcaattgac gaaatcatcg gtcctgtgca attgtccaat
    20161 ggctactttg ccttatggta cgtgatcgag aaccttccta aaaactcgca agtacctctg
    20221 tccgaagtgg cggatgatat cgaagcgcgt ctgcgaacgc ttaaacgcaa agcgcgaacg
    20281 gcggaacttg tagaaaaagc gcgcaaagag gccgagatcg aatacggcga aagaaaacca
    20341 taagtacaaa tacaaaaccc cttttcatac gaaaaggggt tttaaatttc taaacaacaa
    20401 gttttatatc tgcatcaaat actgaaattt taaaaagaag ttggtgcggt cgaggttcgc
    20461 gccgtcataa tttggaaaat ctaaattatt tcgacccata aaattggcgc caagataaaa
    20521 caccgtaaaa gggttgatct tgtaactcat aagtggcgat atggcgtaga aaatttcatg
    20581 tgttttagtt agtatctgtt gattgctcac atcaagtacg atttcgtcgg tatctttatc
    20641 acgttttgca cggtgtgaat tgatagtgcc catctctccg atcaatcgaa ggaacaaccg
    20701 ttttgtgaat tgatactgca atgtattacg atagatcaat tgttgatcaa tcagatcacc
    20761 gccgaaatct tttcggaagc ttgctgaatt gaattcgaaa tcattgcgta aacggctgat
    20821 cggccgaatc gtagcccaaa ctgtcaactt ggtgcctttg actttggata atggattgaa
    20881 tatatcttct gtattgccgg agcgattgat atagctaccg acttcaccgt agacgccacc
    20941 ggacagtttt tgaaaggcat ccgtaccgga ttcaaatgaa atgcgatgta tattattaaa
    21001 ttgtttgcca gcaaaatttt cattattgag tgccgcgacg ctaaaccaaa agtaagtttg
    21061 ttttttgaat tgtaaaaaca cctgaggatt gatccaccag tccttcagtt taccgtcgta
    21121 attgtatttg cgattgaact gcacacgcgg ttcaacacgg gtcaatagcg gatgatcgtc
    21181 atccatataa aaagtgtatc ggttaaatgc accgacctcg cgtacgttgt tattagtgac
    21241 gaaaccttgt tcggaacgga aacccggtga gcgatcattg aaccaagcga aaaaccccca
    21301 gtggcgagct gttctgtcca aagttacacg tgtggcaata ccgttgaatt tttcattgtt
    21361 aaatccatac gttaaggaat cgccaaacgc tacaaatctg tcggtgctcc cgtacagcga
    21421 cgttgcatcc ggttcatccg tagtactagc ggagacaata ccggaaagag tgtaggtatc
    21481 attgagtcgt attttagtgt ctatcgtgcc gacggtattc gaaccgtcgg aagtccggcg
    21541 atctgttcct aaaatgccga cataggtgcc tttggcaagt tcgtatttgg cgcgaagtgt
    21601 attattgatt gcatttttgg tcgttagaat gggttcgcta ctactttctt cctgaggtac
    21661 tatatatact gggttctcat ccatgccaaa taaatagccg tagcttagtc ggtctgtttt
    21721 cccagacaat ttactaagta ataaaggatc attgatgctg cgtgtgtaca tcagattgag
    21781 gtcattgtcc acaaaaaaaa catcgcgtcc ttcaaggaaa aaaggtcgcc tttcgttata
    21841 gtacaatgca aaagcattat tgacgctgat ctgcgttgcg tcggcttcga tctgactaaa
    21901 atcggggtta tatgctaagt ctaaagtcat atccgaagtc actccgtatt tcaaattgaa
    21961 accgaagttt ttatcgtatt tacttttact ccatatccct ttggttgagt cgccgttaaa
    22021 tttgacaaaa cgatcacgcg aacctaaagc ataaggtaaa aattcaatcg gtttgccggg
    22081 acgtttttcc atattcatcg caatttggcc ggcctgtgca tattgatttc cttttccccg
    22141 gtaaatcggt atccaagagt agagaaaacg attttcgcga ggataaactc gccagaggtg
    22201 gattagccaa ttttgatctt gtctgtcggg aaaacgtaag cttttgaacg ggatggcgat
    22261 ttctactgtc caacgatcat cataaatttt agctgccgac tcccatacgg catcaaaact
    22321 ttcgtcctca tttccattga catccacgga aagatcacct tggataccga gggggtttac
    22381 gaaaaactca aaaccattag tttgtgcgcc aaaaggatca atgttgactc cgacgaaatc
    22441 atcgcgaaac atacgatccc ggtcgctcag gttggcccgc agtttatgca tctccggatc
    22501 ataggcgata aaagcaaaat agacatagtc attatcaaac aaaacataac cttgcgtttc
    22561 cactaaaggt tgacgccctt caaccggacc gttttcaaca aaatttccaa atgatgcgcc
    22621 ttgcaaccat gtatcttcaa tttcaccgtc tatgatgagc gccgattcgg ctttcggtac
    22681 attgagtgtc gttttagcgt ttggctggaa ggtggttcca cccgagcgat gcgttttttt
    22741 gcttacagac tcggccagct gtgcagatac atccaaacca aactgcatcg gcaaagtcat
    22801 aagccaggta tgcatcaaca tgcgaatcca agaattgcgg tgcataagat tccttccggt
    22861 atgaagaagt agggtgactg ttacgccctg tcatacggtc gggcggctaa aaggttgcag
    22921 gattttttac aaatgagggc gaagttcttc taaaaactca tacagtataa acccggtaac
    22981 gccccagacg gtgtgttctt ggtaatcgaa ataatggatt tcatacgtac gatcttgcca
    23041 catacgaaat cccttgcgat gatggcgttc atccaaaaga tgggacagcg gtatttcgat
    23101 gatggattcg gtttcatacg gattgatttt ccaatcaaac ggatacggca cgatgccgat
    23161 aaaaggggtt acatgaaatt gagtcggcgt gaccatcgga ctggtttcag ctaaaatttc
    23221 gacagccttt ccgggtatgc cgatttcttc ttctgtttct cgaagtgcag taatttgtaa
    23281 gtccgtatct tccgcatccc gcgcaccgcc ggggaaagca atctgacctt tatgggtgcg
    23341 tacgctttcg gtgcgtttgg ttagtaaaac gtgaattttt tgttctttga gaaagagcgg
    23401 gattaaaaca gcggcttgcg tattctgtcc ggcgggcaat acgcgctttt tgtgatcttc
    23461 gtacacatga cgaatcagag tgcgcaatgt attcaaatct tcaagcggat ggggcatcgt
    23521 aggattctaa catgtgcata atgcgcaata cttcggcgac ggaccaagcc tgtgcgatgc
    23581 agcctttggg taagtgcggc gcatccccgt cggcgatttc cgataatgta cctaagccgg
    23641 cgtcgtggat cagcggttcg atcgtttgca atatcgtccg gatgtgtgtg cgcgcttcct
    23701 cagaaaaatg atgaacgcgc aagtaagctt ccatgtaagg tccgataagc cacagccatg
    23761 ccgtgccctg atgatacgcc gcatcacgat gatacgcatc gccggcgtaa acgcccgtat
    23821 aaccattctc agatgaagca aggctgcgca ggccggctgg cgttctcagt tgcgcatcaa
    23881 cgcagtttat cacggccaga gctttttcat cggataaaag cggaaaaggc aaactgactg
    23941 caaatatctg attggggcga atactcccgt catggccgga cgaattgact acatcataga
    24001 ggcaccggcg ctcttcgttc caatataagg attcaaaaat attttttacc cgatcggcca
    24061 tcgcttcata tgctttagcc gtgtcgtcat ggttaagctg gtttgaaaaa taagccataa
    24121 tccggagtgc gttataccac agggcgttga tttcgaccgg tttgccgatg cgtggtgtaa
    24181 caacccaatc tccgattttg gcatccatcc atgtcagttg tacaccgctg tcgccggcat
    24241 aaagtagccc gtcatcatcc atgtgaatat tataacgggt gccttgcgta tgtgccgcga
    24301 tgatatcttt cagcgtatca aacaaacgat gcttcagaaa ctctgtatct tttgtttttt
    24361 cataatactg ataacaggcc atcacaaacc acaacgtacc gtccacgtta ttgtattccg
    24421 gcgcttcgcc ggcgtccgga aaacgattgg ggatcatacc ctgtgatacg aaaggaagaa
    24481 atgtcgaaat gatagccttt gcgtcttcgg tttttcctgt ggccagcgtg aggcccggca
    24541 aactgatcat cgtgtcgcgc ccccaatcag taaaccagtg ataacctgcg ataatggtct
    24601 taccgctttg tccggtcacc ataaattggt cggctgccaa cgccaatcgc cgggcaagcg
    24661 gatctttcaa cggaaaatcc gacaatattt tgatgcgacg atgaacttca ttagtccaca
    24721 tctcccaaat cgtttcagag gtaaagcggg tattttccag cgaggcagcg acatctaaag
    24781 ctgatccggg ttccaatgtg accgaaagag tgccgtgcgt aaatagatct tcgcgaaact
    24841 cgagaccgcg ttcctgctct ttacggtact cggtgttatt gtaccaataa aatttaggat
    24901 caaaaaaagc gccgggcgcg gtgatgtaca aaggcggcag atggtcgtaa ggacgaatgg
    24961 tcagccgatg ttcggaaaat tctgcttctt tacgaatttg cgatgtttcg cgtgaaaggc
    25021 tgtggtaatc acggaatgca cataaggggc gaagaatcag tgtgacgggc tgactggatt
    25081 cgcgaaggtg ataccaaacg atcacggtat tgcttccatg cggcatgaga atggcttttt
    25141 cgagcagtac accgtccagg tcgtagatca tgcgcggata aagatcgtat tcgaaggacg
    25201 ccatgcgctt atggccatca ggatgaataa taaacggata ttgattggtt gcgaggtcat
    25261 acagtttgtc gccgatacgt acttgctcgt cgtatttgga taaaaagaca aaacgttgaa
    25321 caggcggacg aagcgaggcg cataataaac cgtgatatcg gcgtgtatgc gcaccgatga
    25381 tcgtggacga agcaaaaccg ccaagaccgt tggtttccag ccattccttt tgcgtggcgt
    25441 aataataatc accgcaggtt tcgcggtcca atcgtatggg ttccgggggc gccggttttt
    25501 gcatggtaaa atcctgataa ctaatcggta aacagggaaa tatgcgccat gcggcaactt
    25561 gttattatgg taagaactat ataagttaaa agaaatttga aataaagcat cagaaattaa
    25621 tccgtatgtt caaaggaagc gtgctcatcg tagatgatga agacaatctg cggcaactta
    25681 tggcgcgtat gctggaactt gaagattttg gcgtcattca gtccaaagat aaagcctcag
    25741 ctaaaaccgt acttgagcac gagcctgtgg atgtgatgtt actggatgtc aagctaccgg
    25801 acggttttgc tattgattgg atatccgaac tcaagtcgga gtttccgtgc atggagatcg
    25861 tattgcttac ggcctacggt actatacctg acggtgtcaa agcgataaaa tccggtgcct
    25921 acgaatatat caccaaaggt acggacaacg atcagatact gcctatcgta agccgcgcgt
    25981 ccgaaaaagc acgcctaaat agacgaattc agtttttgca atcatcggca gaagaaaaac
    26041 acgcatttga aaccgtaatc ggtacatcac cggccatccg ggaatgtata aatatagccc
    26101 gacgcgtggc ggaaaccgat actacagtcc tgctttccgg cgaaacggga acgggaaaag
    26161 aaatatttgc acaagcgatt cactacggca gcccgcgtaa aaacaaacct tttgtcgccg
    26221 tgaattgcag cgcgtttagc cgagagcttt tggaatcaga actattcggg tataaagccg
    26281 gtgcatttac gggcgcacat agaaacaaac ggggcctgtt tgaagaagcg gatcaaggca
    26341 ctttgttttt agatgaaata ggcgaactat ccgcggaggc tcaggcgaag ttattgcgtg
    26401 tcatcgaatt gggggagttt attaaaaccg gcgatacccg atcgacgcga gtaaatgtac
    26461 gcattatcgc agcaacgcat cgtgatctag cggccgaatc cgctgcaggg cgtttccggc
    26521 aggatctttt ttatcgcatc tcagtgtttc aagtcaaatt accggccttg cgcgaccgat
    26581 ccgaagatat accggctttt gtagaatatt tcgtccgccg tttttccgaa aaactcaaca
    26641 aacggttgcg ttttgcagat aaaggcgatt ggcttgcact gcaaacatac gactggcccg
    26701 gcaatattcg tgaactgcgt aatgtgatcg aaagagccgt gatattatcc gacggagaag
    26761 aattgtatag tgcgcagttg ttaaggttta tttatgataa tccgtcttcg gattcgaata
    26821 cgatggatct gaagcatgtc gaacgggagc acattcgaaa aatattacaa aaaaccggtg
    26881 gccacaaagc cgatgcggcg cgtcttttgg gtatcgggtt gacgacactg taccggaaaa
    26941 tagaagaata cgctttgaat taaagtgcga atttttttca aaatgaaaaa aagccttcca
    27001 taatggaagg cttttttttt gttgcatcaa tatctcggat gtgaactcta taatacacaa
    27061 tgtttataaa cccggcatct ttggcataag gattggttgt atatcatcgc tgtatcggcc
    27121 atacgctatc accttggaat agtgtctttg atgggtggcc aagtcacaac ataagatgag
    27181 gagatgttat gcagtccgga aatagaggat ttaaggcatt tgcattattg acattatcaa
    27241 cggtcggtgt agtatatgga gatatcggta catcaccctt gtatgctttg cgtgaagttt
    27301 ttttggggca ccatcctatc gtaacatctg ttgaaaatgt attaggcgcg ctatctttat
    27361 ttttctggag cctgactatt gttgtagcat taaaatatca tatactcgta ctgcgtatag
    27421 ataatcaggg cgaaggcggc gtatttgctt tactcgctat tatcaaacaa gccaaattac
    27481 aaggccggtt gactatgatc gtgtcgggcg gattgatcct cggtgcatgt ttattatatg
    27541 ctgacggact tatcacaccg gctatttccg ttttgtcggc gttggaaggt atcgaagtgg
    27601 ccacgccggc tttgagttca tgggtcattc cgctgactat cgcaatcctc atcggattat
    27661 ttctcgttca aaaacacggc acgaccggag tcgggcgatt ttttggtccc ataatggtgg
    27721 tgtggtttgt atcattgatc gtcatcgcct ggccgtatgt atggtccaaa cccgaagtat
    27781 tcaacgccgt gaacccgatg tatgcgatac attttttagc gcaacaaggt atcggcagtt
    27841 ccatgttcat tctgggttct gtttttttgt gtgtcaccgg cggagaggcg ttgtacgcgg
    27901 accttggtca cttcggaaaa catgcgattg ataccgcgtg gtttattttg gtatatccgg
    27961 cgttggtgtt caattatttt ggtcaaggcg cttatttact tagccatgat gttgccgtcg
    28021 gatccaatct tttttattta ttaccctcgg aaacattttt gataccgatg gtaatacttg
    28081 ctacgatggc tacgattata gccagtcagg ctatgatttc gggcgccttt tccctcacgc
    28141 aacaagccat cggtctcgga ttctttccgc ggttaaaaat agtt
//
LOCUS       DAOQGF010000037        26451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_842_length_26451_cov_7.428095, whole genome shotgun sequence.
ACCESSION   DAOQGF010000037 DAOQGF010000000
VERSION     DAOQGF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26451
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_842_length_26451_cov_7.428095"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /gene="uvrA"
                     /locus_tag="PK937_07110"
     CDS             <1..1592
                     /gene="uvrA"
                     /locus_tag="PK937_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNH32374.1"
                     /translation="SRLRPDANCVKVADKTIHEINDMTVGEAVHFFVNIQLSEMDREI
                     ARRPLEEILKRYNYLKNVGLEYLTCNRLAGTLSGGEAQRISLATSLGSALVGSLYILD
                     EPSIGLHPRDNDKLIHILKNLQAIGNTVVVVEHDRDMMASCHEIIDMGPRAGRYGGEV
                     VFQGDHAAICQSASSLTGLYLSGRKEISLPSERRQGRGQSIVLKGAAENNLKRIDVQF
                     PLGMFVCITGVSGSGKSTLIHDILYAAAMRKKGQWSNKIGLHKEILGVEHVDAIEMVD
                     QQPIGRTPRSNPATYVKVFDLIRDVFAATPTAKMRGYQSGTFSFNVPGGRCEVCEGAG
                     QILVEMQFLADVYLECEECKGKRYKKEVLEVHFHDKNIHDVLEMPITEALAFFKAHPK
                     IVKKLQVLDDVGLGYLKLGQAGTTLSGGEAQRVKLAAHLADKSGNHVLYIMDEPTTGL
                     HFEDINKLLTSFNKLIEAGHSIIVIEHNLDVIKCADYIIDLGPEGGDKGGMIVATGTP
                     EEVIQVGNSYTGKHLKPYLKK"
     gene            complement(1668..2054)
                     /locus_tag="PK937_07115"
     CDS             complement(1668..2054)
                     /locus_tag="PK937_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNH32375.1"
                     /translation="MNVLNSTFLLRLAVSITLFMHSVPGMFNGGVNNFGTFYLNEVGF
                     APLGVPLAWAIKLSHVAAIFCLMFEKYVKLASLVTIFVLLAGIVMVHYPDGWYVVGGG
                     RNGMEFNVLLIFVLLAIMFPNGLKKN"
     gene            complement(2156..4720)
                     /locus_tag="PK937_07120"
     CDS             complement(2156..4720)
                     /locus_tag="PK937_07120"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNH32376.1"
                     /translation="MNFFPRISLLYFHLSHLIVLSFFTTLAQTVSDSSFIATQKDSSQ
                     TPGNGVDTMIYYGADTTEGTLNNSTVVLKNNAWVRFKGIEIKAAKITIDQPRKIMIAE
                     AVPDSVDSLGQVARYRGIPEFTESGQMFHGNVMEYNFETKRGKVVMGETKMQDGFYYG
                     STIRKIGDSTLYIRGGRFTTCDEKEPHFFFRTKEMKLTVRDKVVAKPVILYIHEVPIF
                     AIPFGVFPSKGGRASGITPPVYFETPGGERQIRNFGYYWAPNDYFDALLQADYLDKSG
                     YQLHGGGNYAKRYHYSGRMDFSYSQLHSLSTTSYNDNRTTAGALSLTHAHTLSERSNL
                     NADIRYQSSKNYFQNTSINQQQILNRQQNSNISYNNTFDWGGISASASHSRNLDNGQS
                     SLTFPNATISKSSSSVIPKSEERRNEPDRWYHTIRYSYNANLLGRREIANDTSDVQNG
                     YGLRHNAGFNAPFKVFSYFTFNPNINVVETWYDRRHENFGYRANGQDTSVVKRGFYAT
                     HTYSMGASLSTKVYGTSNPNLLGLKTFRHVMTPSVSINYTPDFSESKWGYYESVQDTS
                     GKTVRKDRYGKNLTFGGTPSGRQLSMGFGLAHTFQAKVKTSKKDSTQRDPDVRKIDIM
                     NINNGLSYNFEAEEFKLSNLTTSIAVANDLAKNVSLSMNLTHDFYKFDTTLNRRINKF
                     RNFPRLINAGITGGFSIEGGSGASSTTSGSTPSTTASTTPQGTVTPNYGQSYLPSQRE
                     LPSGVPWNARFNFNYDIGRLDPRNVTKTFSGNISAGTRITQNWQVNYSAQLNLLKEKI
                     VSQSFSLVRDLHCWTMRFDWTPTGPAAGYFFVIQVKSTQLQDIKLQRTDYGNRIFQ"
     gene            complement(4730..5164)
                     /gene="dtd"
                     /locus_tag="PK937_07125"
     CDS             complement(4730..5164)
                     /gene="dtd"
                     /locus_tag="PK937_07125"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNH32377.1"
                     /translation="MRIVAQRVNKAHVSVDGRITGAIEKGLVLLIGIKNGDTSADVKF
                     FAEKCLHLRIFEDAEGKMNRSIIDAKGSILAVSQFTLYADTTKGRRPNFLEAARPELA
                     EPLYNEFVTHLRSHNIHVETGIFGAMMEVEIHNSGPVTIILE"
     gene            complement(5314..6603)
                     /locus_tag="PK937_07130"
     CDS             complement(5314..6603)
                     /locus_tag="PK937_07130"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNH32378.1"
                     /translation="MIKKASLSYDDKVLDLPLITGTENEQAVDIGKLRGATGLVTMDP
                     GYGNTGACHSGITFLDGEKGILRYRGYNIQDLCESSNFQEVSYLLIHGELPSAQQLEE
                     FKRGITRHTMLHESLRKLYDAYPRDAHPMALLSSIVCSLSTFYQDSIDPLNKKHVEIS
                     IFRLLAKIPTIAAYAYKKSIGQPFMYPNNSLSYCANFLNMMFAVPAEPYEVDPEIEKA
                     LDLLFILHADHEQNCSTSTVRMVGSSQANLFAAISAGICALWGPLHGGANQEVLEMLQ
                     EIHNDGGDVKKYVEKAKDKNSTFRLMGFGHRVYKNFDPRATIIKKTCDRVLSKLGVND
                     PLLDIAHQLEEAALKDPYFIEKKLYPNVDFYSGIIYKAMGFPVEMFTVLFAIGRMPGW
                     IAQWKEMMESKETRIGRPRQIYTGSAERPYTPIHKRG"
     gene            complement(6704..10129)
                     /locus_tag="PK937_07135"
     CDS             complement(6704..10129)
                     /locus_tag="PK937_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32379.1"
                     /translation="MNLTRRLLIIAFFFSAISAFGQRLFVRHYSTAEGLPQNQVFAIH
                     QDQHGFLWFGTGGGLSRYDGRQFRNYTKEHGLASNVIRAIHRDKDQNLWLATDEGISC
                     YVAQRDTFISYHSDHIGQGTVRAIAGDDRRLWFATAGGLTRYDFNDHSFTNFTTANGL
                     PANLVQAVTVTAYGKVFIGTNQGLAVMSDPLSDRIEQVPIPGNNKLLLRVEALYCDAR
                     NRIFAGTQGGVIRIDSLKPEIISTADGLTNPVIRGITSDQSGHIWFATEDGLHVMTRS
                     ERPYLFNSYTTRNGFFANQYYAVFQDSENNLWFGSSRGVSKLISADLISYTEAEGLGN
                     DVVIGITKATDKTWYITTNDGISIFREGRFQNLSDTEGLIAKEIWDAATDSAGVIWLA
                     TYRGLQALLPKEPRTRLPIHTKLPRAGIVSSGYYNLTLPPNDFIHNNRIVDVLVDHRG
                     RIWFASTDKGTGYVEINSTGEISVRTFTTADGLYSNNVWMIYEDRRGQIWAGTIGGGI
                     ARWDEVSQRFTSLNKGRGLSDDVALSIYEDRRGGLWIGTERGAMRLAPEIVSGDSFPG
                     DLRSYIRLVSKRDGLSDNTINAIVEDSEGMIWLGTNNGLNQFNPETGLTLQVYNKKRG
                     LIDNEVSTQNALRFYDHETLIVGTSAGITLLPLRSFRSPLPTRPVVLMQEFMAEDNAN
                     KTARLIPIAMRPDTNVSDAFFSTLRTSVPVVSYLENNITFRFVAPSFKDENDVRYRYR
                     LRGFDRFWSDPTPENHVRYTNLNDGDYVFEVIAGNGNGAWSDVPTRVTFSVMTPLWKS
                     WWFITFSITFFSLSIYTAYKFRANIARQRTAELEQKVLQRTRELVVQKETVERILNEL
                     KETQIHLIHSEKMASLGQMVAGIAHEINNPVAFVKGNISLLERRLHDLERVFTSFSDV
                     FDFYDKFNAFSDDLHKDFRAKLEEIDRIIASTRFEKFLGDLPQVVNEMRDGVDRTQKI
                     VEDLRNFSRLDESQFKEISIIDSIESTLNILKNEYKTRITIHRNFGDVPPVYCNPGHI
                     NQVFMNLLQNAFQAIEGDGNVWIRVVAASNNILIAIRDDGKGIPGEIQNRVFDPFFTT
                     KPVGKGTGLGLAISYKIIEAHKGTIFFESTPQQGTEFKITLPIRRLADLQKPE"
     gene            complement(10134..10775)
                     /gene="nth"
                     /locus_tag="PK937_07140"
     CDS             complement(10134..10775)
                     /gene="nth"
                     /locus_tag="PK937_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNH32380.1"
                     /translation="MESIDFKKQRAQRIVRSLARSYPDARCRLTYHDAFELLIKTILS
                     AQCTDERVNFVGETLFRKYRSPEDFIQVPQAELENDIRSIGFFRSKARHIQTCCRTLI
                     QNFNGRVPSTMDELITLSGVGRKTANVILGNIYNIPSLAVDTHVIRISRLLKLTRHTE
                     AESIEADLMKILSPKDWVMFSHRVQMHGRTVCIARRPQCDHCSLAKDCPSANK"
     gene            complement(11024..14839)
                     /gene="smc"
                     /locus_tag="PK937_07145"
     CDS             complement(11024..14839)
                     /gene="smc"
                     /locus_tag="PK937_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNH32381.1"
                     /translation="MYLSRLEVFGFKTFAQKVDLRFDEGITNIVGPNGCGKSNVVDAL
                     RWSLGEQKASILRSEKMENVIFNGTKQRKPLNFAEVSLTIQNTKGILPTEYTEVTITR
                     RIYRSGESEYFLNRLPCRLKDINDLFMDTGMGADAYSVIELKMVEQILSDKTEDRRKM
                     FEEAAGITKYKIRRRQTFNKLQTTKLDLNRANDIIVEIEKKVNSLKRQTQKAKRHMKL
                     SARLKLAEVQLAHHEYQKFAAQLAPMAEKLENLEFVAEQMLGEISKKEAEVEAMNTGL
                     IEREQSLREIQLQLEEQDKKIKTIEDEILVSRERKKNLEAMIRRYAEEKTALEAKREQ
                     LQARITELSATLADLESQYNGMSGTVEAKKTEMLEFDQQIQAKRTELDTVRKQSVQLI
                     DEVAKKRNAFQLTKNNIQNIERKIADLHREGEFHNTSSEDAISALDEKRYEKKKLGEQ
                     VEALRNKITADQSALDTLRHTLDDLRSKKLHDEAEIKALHAKMAIIQKAIENHEGFPE
                     SVQYILSSKPSGIQATVADMIATDDTYKKAIETALGDSFAYLVAGGSDHVHDALTQLN
                     TNKKGSATFLNRQKIGQFKPAFEAEALASVRGEIIGMATELVRCDDPAMARLLLSDVV
                     IVENFEKATRLADQFPAVRFVTLQGEIVKGNYLIKGGGTVKSSDSLIGQREALNKLQD
                     QLSQYEASVRRYQDELQTTDTTLRELTQTIANNQNLLKNTEQKFVALEKEIAQSEYEQ
                     KRSRESLTRNEDALLQAQREMEAFQLTIEDMGPEMDELEGRRGSLELETRRMEQQLAE
                     LEKQRRERSEGFNEMISSFLRMEGEIKTNRNNIESSRTQIADIEATILRREEETVAAE
                     NEIARMGDVFTEREGELITLSRERDVIERNRDAEGTEVARLRETTGKIEAELKKMRRQ
                     REEFLNSRHTMEQETTNLRFELRALEERIKRDHDFDLTKDSIETLILSTEPGSKEEES
                     EEDAPESAPDTAPQEVSGVENQEMNIDEVLESDLVSDASQAPETASFDPEATKLEIDE
                     LRRKLKSIGPVNMEAFAEFQTEKERLDILLKQRQDLLDAEAQLLETINAINEQAQKQF
                     MEVFEKIRANFIKIFTGLFVDSEADLKLEANEDILEANIDILARPTGKKIQHIQLLSG
                     GEKTLTAISLLFAIYLVKPSPFCILDEVDAPLDDTNIDKFTKILKDFSKDTQFIVVTH
                     NKRTMEAARNIFGITMQEAGVSKVVSVKFSNRGDVSKTDDIEQFINENQVEDLTKEVK
                     DTPPSPSVA"
     gene            15724..18792
                     /locus_tag="PK937_07150"
     CDS             15724..18792
                     /locus_tag="PK937_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH32382.1"
                     /translation="MSGSTRLKRLLLVATMVFLLPMYLFAGNTGKITGVVKDKESGDA
                     LPGVNILIEGTSMGATTNAKGEYTILNVPAGVYTVATSMIGYTKLTKQNVRVIPDFTT
                     RLDFEISATSVTGEEQVVVAERPLIQKDQTMTMSVTTAEEIKNLPVRGFQAAANLGVG
                     IVVSNARNLDGGTANVNIRGGRSSETGVYMDGFQQNNLLTGTSNANVPSGAVEEVQVI
                     TGGFDAEYGRNQSGIIQVTTKSGGQRYSGNIEAVGDPAGLGIAESYGYKVLSGGIGGQ
                     VIPGNNTIRFFVSAEAREIDDAEPSVWGYPEYSISKALSPANYYDHDGDPSTPTVPKD
                     TVTWKTNKDGSVKYKKGARPDGVAGAGINSDKGVNLQAKVTFDAIPNKLRIDLTGNFG
                     QTIRRNTTVARTLAVNDNLRREISNLNVGTIATYTLDQKSFVDFGINYFSNTRKLMND
                     LLGFDNVYDYRTRQLGSTGAPTYFNRNVLQDVNRGSLNYRQDVDQYIAFKGNYTNQID
                     KHNQVKIGADFFRHTIRYLNIGDIGNPITGSNDNIGYKLVEKNGKAVIKEVNSDDLEN
                     KQQGPPHPISLSLFGQDKLEYEGLVIRGGLRLDVFNPGVKRVRNIGDPTGSKAEDQQL
                     VFNDLNLNGEFDRASEIATDGYTDRNDNGNYDEGTDRLHAGSIGAEDYTNAKNDVKIS
                     PRFSVSFPISEKTQFRMSYGKFFQQPNLQDLYVSPYYLEGQSTLSFAAATIGNPNLVA
                     EKSTQYEVGIRRALTEKVALDINAYYKDITGLVNSKAVPSEPTGLIIYDNEDQGVVQG
                     VNIGLEVRRVGKFSGRVNYTLQSATGSGSGTNSGFRAAWLGFSDTKFNAPLNFDQRHN
                     INASLDVRNGKGEGPSVGGVKALENAGLNFLVSAGSGFPYTPTTSGALQVFGVPANKV
                     TARRNSQNQPWTFRIDMKADKTFYVSDNMNINVYVQVLNLLDRKNVLVVYPSTGRADD
                     NGFLDTEGFNSTYATQAIRDQVRPQYDVNFHDGFSWDTPRQARLGILFNF"
     gene            18888..22412
                     /locus_tag="PK937_07155"
     CDS             18888..22412
                     /locus_tag="PK937_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32383.1"
                     /translation="MNKRKLIWLLTFVSVVTAVVVSSLYDVSNAKVDTATKLKLRLKK
                     PDIIDLAYVGYNNWSYVMRNNGSFMYDSPDQDGNGNNAGGEFPRGSGTTIVFAAGAWF
                     GTMKNNREVVSECQFASEFQPGRIENSGVPFEELTAEDFLGTEREVYLIDRSGSGDDY
                     NNWPSDAPVDQFGLPGLIADAQTWVVFNDLDTNRSQEGPADSPFPGLGIQITLESFAF
                     NAGSLSNVVFLKFTFDNKTNSDYPNSYLGLWMDADVDNSSNDVIGSDSSKGLGYVYNV
                     DNTDQPYATGFDFFQGPVVSASEVSSFLAFKNADNKTVLKYNPVQGRYVPTTLGGDSV
                     WLGATTLNTYPNSGDPNNNLERYRLLQGIDRDGSVNPNSFNGWYYPGNPLTGIVNVHT
                     VGNDQRILHGVGPFVVKAGAKQEVWAGVIAGIGTDRLSGVADMFKTDVLAQTTFNAGL
                     VAPAPPLTPKINVTGLDGRVNITWSDNAELTYDNAGDVLGIKEDSGYTADYLANDFQG
                     YRVYRSLTGLPGSYERLAQYDKADHITVIRNFTLNTRGSLDIQDINVGDDTGLRYFYV
                     DENVTNLTTYYYSVTAYDAQPYIADEDVLFHDADLGVDIPNPTGLPISLETSPTSNVV
                     SVVPMAATAGSSYDASVTASATHTGPSDGSVDLEVVDRAAVTGHNYRVEFFTIPDSVD
                     GYDLIHPDQGDIAYRVYDITDGVYKKFSTRADNPDTYLDTNANGDKDATDLVWDDSYY
                     ATAQASAADATTEQFHVVDGILVKIFGPALDVKDFLTVANAAGPLNPPVYAAYAFNGS
                     GFPSAQPGFDPVNGVLDRPTDDQQSTNNTKWGFNVGGAGTNFHYNDGTTTSFVARVFR
                     GTNFSRFVPYDFELRFTAAGGQGNFFFSSGNQGAVPFEIWNVGIGTPDNSADDYRMYP
                     RVFDEGGAVDVWDITEFDHPISGGDNDPYTDWIYWMRPTNTNPGQAGYDAVVTAGDGT
                     TGINAEVMARVILVNWNGGSVSDPSYPANLVANRPETGTVFRIVSTKPNQTTDQFAFS
                     TTANTALTSKSAKKNSLDKILVVPNPYYGRSTYQKTLFDKQVMLTNLPPVCTIKIFNV
                     AGDLIATLNHNGASNNKRVNTSPLNLATEASSKTTGAEVWDLRNTNGEFVASGMYIAV
                     VEASGYGKKMVKFAVIQEKTTINGPDIR"
     gene            22521..23549
                     /locus_tag="PK937_07160"
     CDS             22521..23549
                     /locus_tag="PK937_07160"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNH32384.1"
                     /translation="MKSKILALAMVLLYASSTFAGEEKRGTAGFMFLKVPMGAREVSM
                     GQSGITTSMGANAIYWNPANVSMATGPSFQVSYLNHFAGISTNYAALSFPMSDIGTFG
                     LSFNYLSYGDITKTDELHPDGNIGNYSAYDLAFGFTYSKQITDRVNGGMTVKFLNSHI
                     DQVDASAYAFDFGFTYNTDFKGLKMSFVVSNLGPQSTYSGDGLLRETTDPLTGATTYF
                     AYESEPFNLPAAVNFGLSMDLMRNEQNSVTGMLEQNVNSFQVSRTNFGLEYGFQNMFF
                     ARAGYSSALKKDRDYNTGNDAGMSGLTMGAGVDYKFNDSFGATVDYGYLNMGLLGGTH
                     RFSVGLKF"
     gene            23738..25078
                     /gene="gdhA"
                     /locus_tag="PK937_07165"
     CDS             23738..25078
                     /gene="gdhA"
                     /locus_tag="PK937_07165"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNH32385.1"
                     /translation="MSDYVKSVLAQVKAKNPAEPEFHQAVQEVAESLEPVLDRHPEYR
                     TAKILERMVEPERVVMFRVPWQDDQGEIHINRGFRVQMNSAIGPYKGGLRFHPSVNLG
                     ILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELYRHIGADL
                     DVPAGDIGVGGREIGFLFGQYKRLKNEFTGVLTGKGINWGGSLIRPEATGFGVTYFAQ
                     EMLRTKGKDIKGLTFAVSGFGNVAWGAALKISELGGKVVTISGPDGFVYDKDGIQGEK
                     INYMLKMRASGKDRVKDYADEFKVEYHAGKRPWSVKVDVAVPCATQNELGVEDAKELV
                     KNGCICVCEGANMPSTLDAYEVFKQAGILFSPGKASNAGGVATSGLEMTQNSMRLPWS
                     AEEVDQRLRGIMKSIHDTSLHTAKEYGHPGNYVVGGNIAGFLKVANAMMDQGLV"
     gene            complement(25138..25740)
                     /locus_tag="PK937_07170"
     CDS             complement(25138..25740)
                     /locus_tag="PK937_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNH32386.1"
                     /translation="MIRAIFFDLGKVLVDFDWNTAAEHISRKSELKITEIIYRSTASD
                     VAMSFECGQLDSKEFLEKLKLSLRFRGTTDELHFLWTEIFTPIHNNIELLYRLKPTYT
                     LGLISNTNALHFDYIRNTYDFTKEFDRLILSYEVGAMKPARQIFEKAIAGMNLRSEEI
                     LFIDDIAKNLDGAKDLGWQTIHYTGHDELMKKLQTFMVII"
     gene            complement(25737..>26451)
                     /locus_tag="PK937_07175"
     CDS             complement(25737..>26451)
                     /locus_tag="PK937_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32387.1"
                     /translation="TEKQVKLENYTPDSKAHSIPILKLNFVSNFNTGIYSYSPMVSAF
                     MPLDTSRYRYPIKITGSVQEWCGLVYTQINHRNNKFDVQSYSYFEKEGDESYPLEPAW
                     LEDALWTKGRLGPTALPTGRIRIIPGLTQSRFLHRRLGVETAEASLQELPDDRGHAGP
                     IISYKIEYTSYNRTLIINFKKSWPHEITSFEGIYMDGFGEKAKKLHSIGIRTHTIKTN
                     YWTKNTNADSTYRELLGFK"
BASE COUNT         7052 a         5545 c         6343 g         7511 t
ORIGIN      
        1 gctcgcggtt acgtccagat gccaattgtg tgaaagtcgc cgataaaacg attcacgaaa
       61 tcaatgatat gacggtcggt gaagccgtgc atttttttgt aaacattcaa ttatcggaga
      121 tggatcgtga gattgcacgt cgtccgctgg aagaaattct caaacgttat aactatctga
      181 aaaatgtcgg actcgaatac ctcacatgca atcgtctggc cgggacactc tccggcggtg
      241 aagcccaacg tattagtttg gcgacatcgc ttggttcggc gttggtcggc tccttgtata
      301 ttttggatga acccagtatt ggtttgcatc cgcgcgataa tgataaactc attcatatac
      361 ttaaaaacct tcaggcgata ggcaacaccg tcgtcgtcgt ggaacatgac cgggatatga
      421 tggcttcttg ccacgaaatt attgatatgg gtcctcgtgc gggtaggtac ggcggagaag
      481 tggtgtttca gggcgatcat gcggcgattt gtcaaagcgc ttcgtcgctg acaggattgt
      541 atctctccgg tcgaaaagaa atttctcttc cgtccgaacg gcgtcaaggt agaggtcaat
      601 ctatcgttct taaaggtgcc gcagaaaata atctcaaacg aattgatgtt cagtttccgc
      661 tgggcatgtt tgtgtgcatc accggcgtca gcggttccgg taaaagcaca ctgatacatg
      721 acattttgta tgctgcggct atgcgaaaaa aaggtcaatg gtccaataaa atcggattgc
      781 acaaagaaat actcggtgtg gagcatgtgg atgcgatcga aatggtggat cagcagccga
      841 tcgggcgcac tccgcgcagc aatccggcga catacgtcaa agtatttgat ctgattcgtg
      901 atgtatttgc agccacgccg accgccaaaa tgcgcggtta tcagtcgggc accttctcgt
      961 ttaatgtgcc gggagggcga tgcgaagtct gtgaaggtgc gggccaaatt ttggtggaaa
     1021 tgcagttcct cgccgacgtg tatctcgaat gcgaagagtg taagggtaag cgttataaaa
     1081 aagaagtttt agaagtacat tttcacgata agaatatcca tgatgtgttg gaaatgccca
     1141 tcaccgaagc gttggcgttt tttaaagcgc atcctaaaat tgtaaaaaaa cttcaggtgt
     1201 tggatgacgt cggcttgggg tatctcaaac tcggtcaggc gggaacgacc ttgtccggcg
     1261 gcgaagcgca acgcgtcaaa ttggcggctc atttggcgga caaatcgggc aatcacgtgc
     1321 tgtatattat ggatgaaccg acaaccggtt tgcattttga agatatcaat aaactgttaa
     1381 cgagttttaa caaactgatc gaagccggtc attccatcat cgtcatcgaa cacaatcttg
     1441 atgtaatcaa gtgtgccgac tacatcattg atctcggtcc ggagggcggc gataaaggcg
     1501 gtatgatcgt tgcaaccggc acacccgaag aagtaataca ggttgggaat tcatataccg
     1561 gaaaacatct gaaaccgtat ctgaagaagt aaaatcgaaa acattaaaaa caaaaacgct
     1621 gcacaaacca tgcagcgttt tttacaagga agattaaaaa gtatacgtca atttttcttt
     1681 agcccattgg gaaacataat agccagtaaa acaaaaatta gaagtacatt aaattccatt
     1741 ccgttacgtc cgccgccaac cacataccag ccatccggat agtgtaccat cacgataccg
     1801 gcgagcaaga caaagattgt tacaagcgaa gctaacttta cgtatttctc aaacatcagg
     1861 caaaaaattg ccgctacatg cgacagcttg atagcccacg ccagcggaac acctagaggt
     1921 gcaaacccga cttcgttgag gtaaaaagta ccgaaattat tgacgccgcc gttaaacatg
     1981 cccggtacgc tgtgcatgaa taacgtaatc gaaacggcaa ggcgcagcaa aaaagtgctg
     2041 ttcaaaacat tcataactcc tccgacatgg ataatgatga gaataagatt aagttgttta
     2101 cgaattacaa cgagacaagt ttcttatttt ttctaaagca cgattttttc aatcgctatt
     2161 ggaaaatgcg attgccatag tcagtgcgtt gcagtttgat atcctggagc tgcgtagatt
     2221 tgacctgaat gacgaaaaaa taacctgcgg ccgggccggt aggtgtccaa tcaaaacgca
     2281 tcgtccagca atgaagatca cgaacaagtg agaaactctg actgacgatt ttctctttga
     2341 gtaagttgag ctgagcggag tagttgactt gccagttttg ggtaatgcgc gtacctgcac
     2401 tgatattgcc actaaatgtt ttagtgacat tgcgcggatc aagtcgtcca atatcataat
     2461 taaaattaaa acgtgcattc cacggaacgc cgctcgggag ctcgcgttgt gacggtaagt
     2521 aggattgccc gtagttgggt gtcaccgtgc cttgcggtgt agtcgaggcc gttgtagaag
     2581 gtgtagaacc cgatgttgtt gaagatgcgc cggaaccgcc ttcgatgcta aatcctccgg
     2641 tgatacccgc attgatcaag cgcggaaagt ttctgaattt attaatacga cggttgagtg
     2701 tcgtatcgaa tttgtaaaaa tcatgggtga gattcatgga caaacttacg ttttttgcca
     2761 aatcattggc taccgcgata gaagtagtca gattagacag tttaaattct tcggcttcaa
     2821 aattgtagct aaggccatta ttgatattca taatgtctat ttttcttaca tcaggatcgc
     2881 gttgcgtgct gtcctttttg gaagttttga ctttagcttg aaatgtatgc gcgagcccga
     2941 agcccatgga gagctggcgt ccggaaggtg ttccgccaaa cgtcaggttt tttccgtagc
     3001 ggtctttacg tacggttttc cccgacgtat cttgaaccga ttcataatat ccccattttg
     3061 attcggaaaa atcaggcgtg taattaatac tcacactcgg agtcatgaca tgacgaaaag
     3121 tttttaaccc caacagattg ggattgctcg taccgtaaac tttcgtcgaa agcgatgcgc
     3181 ccatgctata cgtgtgcgtg gcataaaatc cgcgtttgac gacgctggtg tcctgaccat
     3241 tggcgcgata gccgaaattc tcatgacggc gatcgtacca cgtctcgacg acattgatat
     3301 tggggttgaa tgtaaaatac gaaaacactt tgaatggcgc attgaatcct gcattgtgcc
     3361 gtagaccgta cccgttttgc acgtctgaag tatcattggc gatttcacgt cgtccgagta
     3421 ggttggcatt atacgaataa cgaatcgtgt gataccagcg atccggctcg ttgcgacgtt
     3481 cttcggattt gggtatcacg gaggatgagg acttgctgat cgtcgcattg ggaaatgtaa
     3541 gcgaactttg accgttgtcc aaattacgag aatgcgatgc gctggccgat ataccacccc
     3601 agtcgaatgt attattgtaa ctgatattcg aattttgctg acgatttaat atttgctgtt
     3661 ggttgatact ggtattttga aaataatttt tactgctctg gtagcgaatg tcggcgttga
     3721 gattggatcg ttcgctcaaa gtatgcgcat gggtgaggct gagtgccccg gcggttgttc
     3781 ggttatcgtt ataacttgtc gtgctaagtg aatgcaattg agaataagaa aaatccattc
     3841 gtccggaata atgataacgt ttggcgtaat tgccaccgcc atgaagctga taaccggatt
     3901 tgtctaaata atcggcttgc aggagcgcat caaaatagtc attgggtgcc caataataac
     3961 cgaaattgcg aatttgtctc tcgccgccgg gtgtctcaaa ataaacggga ggagttatac
     4021 cggaagcgcg tccgccttta ctgggaaata caccaaacgg aatggcgaaa atcggcactt
     4081 catgtatgta tagtatcacc ggtttggcga cgactttatc gcgcacagta agtttcattt
     4141 ctttggtgcg gaagaaaaaa tgcggttcct tctcatcgca tgtcgtaaat cgtccgccgc
     4201 gtatatataa tgtactgtcg ccgatttttc gtatggtaga gccgtaataa aaaccatcct
     4261 gcattttggt ttcacccatg acgactttac cgcgcttggt ttcaaaattg tactccatca
     4321 cattgccgtg aaacatctgg ccggattcgg tgaattcggg tataccgcga tagcgcgcca
     4381 cttggccgag tgaatcgacg gagtcgggta cagcctcggc gatcatgatc ttgcggggct
     4441 gatcaatggt aattttagcg gctttgattt caatcccttt aaaccggacc catgcattgt
     4501 ttttgagaac cacggttgaa ttattcagtg tgccctccgt agtatcagcg ccgtagtaga
     4561 tcatcgtatc cacgccgtta ccgggagttt gggaagagtc tttctgtgta gcaataaacg
     4621 aagaatcgga aaccgtttgt gcaagcgttg tgaaaaagct caatacgatc aagtgtgaaa
     4681 gatggaaata caatagtgag atgcgtggaa aaaaattcat gcgtagtttt tattccaaaa
     4741 taatcgtgac cgggccggag ttgtgaattt ccacttccat catggcgcca aaaatgccgg
     4801 tttcaacgtg tatattgtgc gatcgtaagt gcgtgacaaa ttcattatac agcggttcgg
     4861 ctaattccgg cctcgcggct tccaaaaaat taggccgtcg tcctttggtt gtatcggcat
     4921 agagcgtaaa ttgagaaact gcgagtatag agcctttggc gtctataata ctgcgattca
     4981 tttttccttc ggcatcttcg aagatgcgga ggtggaggca tttttccgca aaaaatttta
     5041 catcagccga tgtgtcgccg tttttaatgc cgatgagtaa aacgaggcct ttttcgattg
     5101 caccggtgat gcggccatct accgatacat gggctttgtt aacacgctgc gctacgatac
     5161 gcatgatttt tcctatagta aaagaaaata aaacgaagtt gttgtgcgag cgaagatcgt
     5221 gacgcaatat gttcaattta tctaatttct tcaaactgaa gataaaaaaa accggcatga
     5281 aattatgccg gtttttagtt tcagggaaaa aggttatccg cgtttgtgaa tcggcgtata
     5341 agggcgctct gccgatccgg tatagatctg gcgcgggcgt ccgatgcgcg tttctttgga
     5401 ttccatcatt tctttccatt gagcgatcca tccgggcata cgtccgatgg caaaaagcac
     5461 cgtaaacatt tcgacaggga accccatggc tttgtaaata ataccgctgt agaagtctac
     5521 gttggggtac aatttctttt cgatgaaata cggatctttt aaagcggctt cttcgagttg
     5581 gtgcgcgatg tcgagcaaag gatcatttac accaagtttc gacaatacac ggtcgcaggt
     5641 ttttttgatg atcgttgcgc gcgggtcgaa gttcttatat acacgatgac cgaaccccat
     5701 caaacggaat gtggaatttt tatctttagc tttttccaca tattttttga catcaccgcc
     5761 gtcgttgtga atttcctgca gcatctcaag cacctcttga ttggcgccgc cgtgaagcgg
     5821 gccccacagt gcacaaatgc cggctgaaat agccgcaaaa agattggctt ggctgctgcc
     5881 taccatacgt acagttgacg ttgaacagtt ttgttcgtga tcggcatgta aaataaaaag
     5941 cagatcaagc gccttttcga tttccggatc cacttcatac ggttcggcag gaaccgcaaa
     6001 catcatattc agaaaattgg cacagtaact gagcgaatta ttcggataca tgaaaggctg
     6061 accgatggat tttttgtaag cataggccgc tatcgtggga attttggcaa gcaaacggaa
     6121 aatcgaaatt tccacatgtt ttttattcaa cggatcaatg gaatcctgat aaaacgtaga
     6181 tagcgaacac acgatggaag acaaaagcgc catcggatgg gcatcccgcg gataggcatc
     6241 gtaaagcttg cgcagcgact cgtgcagcat ggtatggcgc gtgataccgc gtttaaattc
     6301 ttcaagttgt tgtgccgacg gcagttcgcc atgaatcaac aggtagctga cttcttggaa
     6361 attggaactt tcgcacaaat cctgaatatt atatccgcgg taacgaagaa tacctttttc
     6421 gccgtcaaga aacgtgatac cggaatggca tgcgccggta ttaccgtaac ccgggtccat
     6481 ggtgacaagt cccgtagcgc cgcgcagttt gccgatgtcc acggcttgtt cgttttcagt
     6541 gccggtgatc agcggtaaat ccagcacttt atcatcgtaa gataatgaag cttttttaat
     6601 catatagact ccttagaacg tttggagtgg cggtaagttg atgtggttaa aaagaaagtt
     6661 aaaatcgaaa aaaagataat acatctctta ttttgataca agcttattcc ggcttttgga
     6721 gatcggctaa gcggcgtatc ggtaatgtta ttttaaattc agttccctgc tgcggggtgc
     6781 tctcaaaaaa aatcgtgcct ttgtgcgctt cgatgatttt gtatgaaata gccagaccga
     6841 gccccgtacc tttaccgacc ggttttgttg tgaaaaaagg atcaaaaacg cggttttgaa
     6901 tttctccggg aatgccttta ccgtcgtcgc gaatagcgat caaaatatta ttgctggcgg
     6961 caacaacgcg aatccaaacg ttgccgtcac cttcgatcgc ttgaaaagca ttttgcagca
     7021 aattcataaa aacttgattg atatgtcccg gattgcaata caccggtggc acgtctccga
     7081 aattgcgatg aatggtaata cgcgttttat actcgttttt gagtatgttg agtgtggatt
     7141 caatgctatc aatgatggaa atttctttga attgcgattc gtccaggcgt gaaaaatttc
     7201 gcagatcttc tacgattttt tgcgtgcgat ccactccgtc ccgcatttcg ttgacaactt
     7261 gaggcagatc gcctaaaaat ttttcaaatc gcgtcgacgc aataatacga tctatttctt
     7321 ccagtttggc gcgaaaatct ttgtgtagat cgtcgctgaa ggcgttgaat ttatcgtaga
     7381 aatcaaaaac atcggagaaa ctggtaaata cgcgttccag atcatgaaga cgccgctcta
     7441 ataatgaaat atttcctttg acgaaagcga ccggattatt gatctcatgc gcaatgccgg
     7501 ctaccatctg accaagcgaa gccatttttt cggagtgtat cagatggatc tgcgtttctt
     7561 tgagttcgtt caaaatacgc tcgacggtct ctttctgtac gaccaattcg cgtgtacgct
     7621 gcaatacttt ttgttcaagt tcggcggtgc gttggcgtgc gatattggcg cggaacttat
     7681 aagcggtgta gatgcttaaa ctgaaaaatg tgattgaaaa agtgatgaac caccagcttt
     7741 tccatagggg cgtcatgacg gaaaacgtta cgcgggtggg aacgtcgctc caggcgccat
     7801 tgccgttgcc ggcgataacc tcaaatacgt aatcaccgtc gttgaggttg gtataacgca
     7861 catgattctc cggtgtcggg tccgaccaaa atcggtcaaa gccgcgtaaa cggtaacggt
     7921 accgcacatc attttcatct ttaaacgatg gcgctacaaa acgaaatgtg atgttgtttt
     7981 cgagatagct gaccaccggt acggatgtac gaagtgtact gaaaaatgca tccgaaacgt
     8041 ttgtatcggg gcgcatagca atagggatca accgggctgt tttgttggca ttatcttcgg
     8101 ccataaattc ttgcatcaaa acaacaggtc gggtcggtag gggcgagcga aatgaacgta
     8161 atggcagcaa cgtgataccg gcactggtac cgacgatcaa ggtctcgtga tcataaaagc
     8221 gcagtgcgtt ttgtgtactt acttcattgt caataaggcc tcgctttttg ttgtaaactt
     8281 gaagcgtcag cccggtttcg ggattaaatt gattaagccc gttattcgtg cccagccaaa
     8341 tcatgccttc cgaatcttcc acaatcgcat tgatggtatt atcggacaaa ccgtcgcgtt
     8401 tagacaccaa tctaatgtaa gaacgcagat cgccgggaaa agaatctccg ctcacgattt
     8461 ccggcgccaa acgcatcgcg ccgcgctcag taccgatcca taaaccgccg cgtcggtctt
     8521 cgtaaatact taatgcgaca tcatccgaaa gcccgcgacc tttgttgagg ctggtaaaac
     8581 gctgagatac ctcatcccaa cgcgctatac cgccgccgat cgtgcccgcc catatttgcc
     8641 cgcgccggtc ctcatagatc atccatacgt tgttactgta caaaccgtct gcggtagtaa
     8701 atgttcgaac ggaaatttca ccggtcgaat tgatttcaac gtaacccgtt cctttatccg
     8761 tgctggcaaa ccaaatacgt ccgcgatggt caaccaaaac gtccacgatg cggttattat
     8821 gtataaaatc gttaggtggt aaggtaagat tgtaatatcc ggatgacaca atgcctgcgc
     8881 gcggaagttt ggtatgtatc ggaagccgcg tgcgcggttc tttgggaagc aaagcctgaa
     8941 gaccgcgata ggtcgctaac catattactc ccgccgaatc cgttgcggca tcccatattt
     9001 ctttggcaat gagaccttct gtatctgaca gattttgaaa ccgtccttcg cgaaaaatgg
     9061 aaatgccgtc gttggtagta atataccatg ttttatccgt agcctttgtt atgccgatca
     9121 cgacatcgtt gccaagacct tctgcttcgg tgtatgaaat gaggtcagcg gaaatcagtt
     9181 tactgacacc gcgggaggag ccaaaccaaa ggttgttttc ggaatcctga aaaacagcgt
     9241 aatattgatt ggcaaaaaaa ccattgcgag tggtgtagct gttaaataag taagggcgct
     9301 ctgatcgggt catgacatga agcccgtctt cggtagcaaa ccagatgtga ccggattggt
     9361 cgcttgtaat acctcggatg acaggattgg tcagaccgtc cgctgtgctg atgatttccg
     9421 gtttgagact gtcaatgcgt atgacgccgc cttgcgttcc ggcaaaaatg cggttgcgcg
     9481 catcacaata aagcgcttcg acacggagaa gtaatttgtt atttccgggg attggaactt
     9541 gttcgatacg atcactgagt ggatcggaca tcacagccag tccctgattg gttccgatga
     9601 aaacttttcc gtaggctgtt acagtaacgg cttgaacgag attggcaggt aaaccgttgg
     9661 ccgtggtaaa gtttgtaaac gaatgatcgt taaaatcata acgcgtaagc ccaccggccg
     9721 tagcaaacca taaccgacga tcgtctccgg ctatagcgcg cacggtacct tgtccgatat
     9781 gatcggaatg ataactgata aatgtgtcgc gttgagctac ataacaactg ataccttcat
     9841 cggtagccaa ccaaaggttt tgatctttgt cgcgatgaat ggcgcggatg acattactgg
     9901 ctaggccatg ttctttggtg taattgcgaa attgacgccc gtcgtatcgg ctgaggccgc
     9961 cgccggttcc aaaccaaaga aaaccatgtt gatcctgatg aatggcaaaa acctgatttt
    10021 gaggcagccc ttcggcggtc gaataatggc gaacgaagag gcgttggcca aatgccgaga
    10081 tagcgctgaa aaaaaaggca atgatcagga gacggcgcgt aaggttcatt tttttattta
    10141 tttgcggacg ggcaatcttt agctaaggaa caatgatcgc actgagggcg acgggcaata
    10201 cacaccgttc gaccgtgcat ttgaacgcgg tgtgaaaaca tgacccaatc tttaggcgaa
    10261 agaattttca taagatcggc ttcaatggat tcggcctccg tgtgccgcgt tagtttaagc
    10321 aaccggctga tccttataac gtgtgtatcc acggccaagg atggaatatt ataaatattt
    10381 cccaaaataa cattagctgt ttttcgtcct acgccggata aggtgatcag ttcatccata
    10441 gtactcggaa cgcgtccgtt gaaattttgt atgagcgtgc ggcaacacgt ttgaatgtgc
    10501 cgcgctttac tccgaaaaaa accgatggag cggatatcgt tttcaagttc ggcctgtgga
    10561 acctgaataa aatcttcagg agaacggtat tttcggaaaa gtgtttcgcc gacaaaattg
    10621 acacgctcat cggtgcattg ggcagagagg atagttttga taagcagctc aaacgcgtca
    10681 tgataggtca gtcggcatcg tgcgtcagga tacgagcggg caagcgaacg cacgatacgc
    10741 tgtgcacgct gttttttaaa gtctatagat tccatagtga gagccgaata cgatagccgg
    10801 tattttgaaa atataagatg ttcgcacggt gttagccaat aaaaaattga tatgataagt
    10861 tgtgcgatga atttcaaaaa tagtaacaaa gcttaacaag gagcaggaat aagtacaggc
    10921 gagataaaaa atatgatcag aacgcagtaa tgcgtaaaat tattgcgcaa attcgtagat
    10981 tgcgacggat cggtgaatgg gtggacggtg tacttttacg gtatcacgca acgctcggcg
    11041 acggcggtgt gtctttgact tctttcgtca ggtcttcgac ctgattttcg ttgataaact
    11101 gctcgatatc gtcagttttg ctgacatcgc cgcgattgga aaatttaacc gatacgactt
    11161 tggatacgcc ggcttcctgc atggtgatac caaaaatatt acgcgccgct tccatcgtac
    11221 gcttattgtg cgttacaacg ataaactggg tgtctttgga gaagtctttg aggatcttag
    11281 tgaatttatc aatattggtg tcatccagcg gcgcatccac ttcatccaaa atacagaatg
    11341 gactgggttt gacaaggtag atggcaaaaa gcaaagaaat agccgtcaat gttttttcac
    11401 cgccggagag cagctgaatg tgttggattt ttttgccggt cggtcgggct aaaatatcaa
    11461 tattggcttc cagaatatct tcattggctt ccagcttgag atcggcttcg gaatcaacaa
    11521 acaaacccgt aaatattttg ataaaattgg cacggatttt ttcaaagact tccatgaact
    11581 gtttttgtgc ctgttcattg atggcattga tcgtttccaa aagttgcgct tcggcgtcca
    11641 gcaaatcttg acgctgtttg agcaatatat caaggcgttc tttttccgtt tgaaattccg
    11701 caaaggcttc catgttaact gggccgatac tcttgagttt acggcgcagt tcatctattt
    11761 cgagtttagt cgcctccgga tcaaaagatg ccgtctcagg tgcttgcgat gcatcggaaa
    11821 cgagatcgct ttccagtact tcatcaatat tcatttcctg attttcgacg ccagatactt
    11881 cctgcggcgc ggtatccgga gcgctctccg gtgcatcttc ttcggattcc tcttctttac
    11941 tcccgggttc cgtactgagg atcagtgttt cgatggagtc tttggtcaag tcaaaatcgt
    12001 gatcgcgttt gatgcgttct tccagcgcac gaagttcaaa acgaagattg gtcgtttctt
    12061 gctccatagt atggcgtgag ttgaggaatt cctcacgctg acggcgcatt tttttcaact
    12121 cggcttctat ttttccggtc gtttcacgca gacgggcgac ttcggtacct tcggcatcgc
    12181 ggttgcgttc gatcacgtcg cgttcacgcg aaagagtgat gagttcgcct tcgcgttcgg
    12241 tgaaaacgtc acccatacgg gcaatttcat tttcagcggc gacagtttct tcttcgcgac
    12301 gaagaatggt tgcttcaata tctgcaattt gtgtgcggct ggattcgata ttgttgcggt
    12361 ttgttttgat ttctccttcc atacggagaa aggatgaaat catttcgttg aatccttcgg
    12421 aacgttcgcg ccgctgcttt tccagttcgg cgagctgctg ttccatgcgc cgggtttcaa
    12481 gttcgagcga accgcgccga ccttcgagct catccatttc agggcccata tcttcgatgg
    12541 taagctgaaa cgcttccatc tcgcgttgtg cttgcagaag cgcatcttca ttgcgtgtca
    12601 aggattcacg ggaacgtttt tgttcgtatt cggattgggc tatttctttt tccagagcga
    12661 caaatttttg ctcagtattc ttcagaagat tttgattatt tgcgatcgtc tgggtgagtt
    12721 cgcgcagcgt agtgtcggta gtctgcagtt cgtcctgata acggcgaacg gaggcttcgt
    12781 attgggataa ctgatcctgc agtttattca aagcttcgcg ttgaccgata aggctatcgg
    12841 atgatttgac ggtaccgccg cctttgatga gataattgcc tttgacgatt tcaccttgca
    12901 atgtgacgaa acgtacagcg ggaaattgat cggcaagccg cgttgctttt tcaaaatttt
    12961 cgacgatcac cacatcgctc aaaagcagac gcgccattgc cggatcatca catcgcacca
    13021 gttcggtagc cattccgatg atctcgccgc gtacggaagc aagtgcttcg gcttcaaaag
    13081 caggtttgaa ttgcccgatt ttttgacgat tgaggaatgt cgcactgcct tttttgttgg
    13141 tattgagctg ggtcagcgcg tcgtgcacgt gatcacttcc gccggccaca aggtaggcga
    13201 aggaatcgcc gagtgcagtt tcgattgctt ttttgtacgt atcatccgtc gcgatcatat
    13261 cggcgaccgt tgcttgaatg ccggacggtt tggaagatag gatatactga acgctttcgg
    13321 gaaacccttc atgattttcg atggcttttt gaataatagc catcttggca tgcagcgctt
    13381 tgatttcggc ctcatcatgc agctttttag agcgcaaatc gtccagtgta tggcgaagcg
    13441 tatctaaagc cgattggtca gccgttattt tattacgcaa cgcttcaact tgttcgccga
    13501 gttttttctt ttcatagcgt ttttcatcca gcgcgctgat cgcgtcttcg gatgaggtgt
    13561 tatgaaattc gccttcgcga tgcagatcgg cgattttgcg ttcgatattc tggatattgt
    13621 ttttagtgag ttgaaacgca ttgcgttttt tagccacttc gtcgatgagt tgtacggatt
    13681 gtttacgtac ggtatccagt tcggtacgtt tggcttgtat ctgctggtca aactccagca
    13741 tttccgtttt tttggcttcg acggttccgg acataccgtt gtattgcgat tccaaatcgg
    13801 cgagcgtcgc ggagagttcg gtgatgcggg cttgcagttg ttcgcgcttg gcttccaatg
    13861 cggttttttc ttctgcatag cgacgtatca tcgcttcaag attttttttg cgctcgcggc
    13921 tgacgaggat ttcatcctcg atcgttttga tttttttatc ctgctcttcg agttgcagtt
    13981 ggatctcacg taaactttgt tcacgttcga tcaggccggt attcatggcc tcgacttcgg
    14041 cttctttttt ggaaatttca cctaacattt gttcggcgac aaactcgagg ttttctaatt
    14101 tttcggccat cggcgccagt tgcgcggcaa acttttggta ttcatgatgc gccagttgga
    14161 cttccgccaa tttgagacgg gcggacagtt tcatgtggcg cttggctttt tgggtttggc
    14221 gcttgaggga attgaccttt ttttcgattt cgacgatgat atcgttggcg cggttgagat
    14281 cgagtttggt cgtttgcagt ttgttaaacg tctgacgacg gcgaattttg tatttggtga
    14341 tacctgccgc ttcttcgaac attttccggc ggtcttccgt tttatcggac aagatttgtt
    14401 cgaccatctt gagctcgatt acggagtacg cgtccgcgcc cataccggta tccataaaaa
    14461 gatcattgat atctttgagg cggcaaggga ggcggttgag aaaatattcg ctttcaccgg
    14521 atcggtatat ccgacgggtg atcgtgactt cggtatattc cgtcggcaga atacctttag
    14581 tgttttgtat ggtcaggctg acttcggcaa aattaagggg cttacgttgt ttggttccgt
    14641 taaaaataac gttttccatt ttttcggaac gcaggatact ggctttttgt tcaccgagtg
    14701 accagcggag ggcatctact acgttggact taccgcagcc gttgggaccg acgatattgg
    14761 tgataccctc gtcaaaacga aggtccactt tctgagcaaa tgttttaaaa ccgaaaactt
    14821 caagccgtga caaatacatg ccgaataatc cttataaacc taaaaaaatc aaaacttaaa
    14881 taagtttcct gtcaatcagg gaatcatctt ttagcaaact aacattgtat tattatagta
    14941 tacccatact aatcataata tagtatatcc atactaaata acacgaaaaa cgtatcattt
    15001 cgctttgggg cggctaatat atagattaaa tcgtgctggc gcaaaatctt tttaaaaaac
    15061 tttgtaataa aaccacaacg catcgtactt ctattattac ggtaccggta ttgcgaatat
    15121 gggcatgaaa agagcgtgtt ttgcccggat tctacgtatg aatgaaaaaa tttaaaaaaa
    15181 tatcagaagg gggtgggaaa cttatttctt tcattttttc ggtagttttg taatctaagg
    15241 cgggaatatt atctggggat taaggtttta ttgattagct caaagtgccc atcgtggtct
    15301 taaaaccaag taaaaccggg ttcccaaaaa aagcttgaca ataattttaa ggccgcctat
    15361 attgtttatt gtaaaacctt atcttgtgca ataagtgtcc ctagtctttt caaaagcttg
    15421 ttgccgggtt gtaaacaaat ctttttaata acgttctctt aaaactgttc tctgaaagca
    15481 gtatccttga gtatgatgtg aaggagaggg cggcacgctt gtaaccgtag tgctgattct
    15541 ttcacaaaaa aacgaaaggg taggtagaat taacctcaga agcagatgtc aagtcggaaa
    15601 aacacatttc ttatctatgt gcatttacaa aggaggtaaa tgaaaaaagg tatgattact
    15661 aaacttatcg aaaaaaaatc attcatcgta cacgctctta ataaccaaca gaaggaggac
    15721 attatgtctg gtagtacgag actaaaacga ttgttgctgg tggcaacaat ggtgttcttg
    15781 cttccgatgt acctgtttgc aggtaatacg ggcaagatca ccggtgtggt aaaagacaag
    15841 gaatcgggtg acgcacttcc gggtgtgaat atcctgatcg agggtacttc catgggcgca
    15901 acgaccaacg ccaaaggtga gtacacgatc ctcaatgttc ccgccggtgt ttatacggtg
    15961 gccacaagca tgatcggtta cacgaagttg accaaacaaa acgtccgtgt gattccggac
    16021 tttacgacac gtttggactt cgaaatttct gctacgtcgg taaccggcga agagcaggta
    16081 gttgttgccg aacgtccttt gattcaaaaa gatcaaacga tgacgatgtc cgttacgacg
    16141 gctgaagaaa tcaagaacct gccggttcgc ggtttccaag cggcggctaa ccttggcgtc
    16201 ggtatcgtcg taagcaacgc acgtaacctt gacggtggta ccgccaatgt caatatacgc
    16261 ggcggtcgct cttcggaaac cggtgtttat atggacggtt tccaacaaaa caatcttttg
    16321 acgggtactt ccaatgccaa tgtaccttcg ggtgccgtag aagaagtgca agttattacc
    16381 ggtggttttg atgcggaata cggacgtaat cagtcgggta ttattcaagt aactactaaa
    16441 tccggcggtc aacgctattc cggtaacatt gaagccgtgg gcgatccggc aggtctcggt
    16501 atagctgaaa gctatggata taaggttcta agtggtggta ttggtgggca ggttattcct
    16561 ggaaacaaca ccattcggtt cttcgtatca gccgaagcgc gtgaaataga tgatgctgag
    16621 cccagtgttt gggggtatcc ggagtattca atatccaaag cattgagtcc ggcaaattac
    16681 tatgatcacg acggtgatcc atcaactcca accgttccta aagataccgt aacttggaaa
    16741 accaataaag atggatcggt gaagtataaa aaaggcgctc gtccggatgg agtcgccggc
    16801 gctggtataa actcggataa aggcgttaat cttcaggcca aagtcacttt tgatgctatt
    16861 cctaataaat tacgtataga tttgacagga aatttcggcc agacaatacg tcgtaacact
    16921 acagttgcgc gtacgttagc ggtcaatgat aatctccgcc gagagatttc aaatttgaat
    16981 gtgggaacta tagctaccta cacattagat caaaaaagtt ttgtcgattt cggtattaat
    17041 tacttctcga ataccagaaa actgatgaac gatttgttgg gttttgataa tgtatacgat
    17101 tatcgcacga gacagcttgg ttctacaggt gcgccaactt attttaaccg aaatgttctt
    17161 caagacgtga accgtggttc gctaaactac cgtcaagatg ttgatcagta tatagcattt
    17221 aaaggcaatt ataccaatca gattgacaaa cataatcaag tcaaaatcgg agctgatttt
    17281 ttccgccata ccatacgtta tttgaatatc ggtgatatcg gcaacccaat cactgggtca
    17341 aacgataata tcggttacaa actggttgaa aagaacggga aagctgtcat caaagaagtt
    17401 aatagcgacg atcttgaaaa taaacagcag ggaccgccgc acccgatcag cctctctcta
    17461 tttggacaag ataaacttga gtatgaaggc ttagtcattc gtggtggtct gcgtttggat
    17521 gttttcaatc ccggcgttaa acgtgtacgt aatattggcg atcctacggg ctcaaaagcg
    17581 gaagatcaac agcttgtttt caacgatctt aatcttaatg gcgaatttga ccgtgcttcg
    17641 gaaattgcaa cggatggtta tacggatcgg aatgataatg gaaattatga tgaaggtact
    17701 gaccgccttc acgccggttc gatcggcgct gaggattata caaatgcaaa aaatgacgtg
    17761 aaaataagtc cgcgtttctc ggtcagtttc ccgatatctg aaaaaaccca attccgtatg
    17821 agctacggga agtttttcca acagcccaat cttcaggatc tgtatgtttc tccttattat
    17881 cttgaaggtc aatcaacgct ttcttttgca gcggcgacta taggaaatcc aaacttggtt
    17941 gcagagaaga gcactcagta tgaagtgggt attcgtcgcg cgcttactga aaaagttgcg
    18001 cttgatatca atgcctatta caaagacatc acgggattgg ttaattctaa agcagttcct
    18061 tcagagccca caggcttgat tatttatgat aatgaagatc aaggtgttgt acaaggtgtg
    18121 aatattggcc ttgaggtgcg tcgcgtcggt aagttctcag gtcgagtaaa ttatacttta
    18181 cagtcggcga ccggtagcgg ttccggaact aattcaggtt tccgtgcagc ttggttggga
    18241 ttctctgata cgaagtttaa tgcgcctctc aattttgatc agcgccacaa tatcaatgcg
    18301 agcctagatg ttcgtaatgg taaaggggaa ggtccttctg tcggaggcgt caaagccctc
    18361 gaaaatgcag gcctgaactt cctagtttcc gcaggtagcg gttttcctta tacgcctacg
    18421 acgtcgggag ctttgcaggt atttggcgtc cctgccaata aagtcacggc tcgccgtaat
    18481 tcacaaaacc agccgtggac attccgtatt gatatgaaag ccgataaaac cttttatgtc
    18541 agcgataaca tgaatatcaa tgtttatgtc caagtgttga atttattaga tcgcaaaaat
    18601 gtgctcgtcg tctacccctc caccggtcga gccgatgata acggatttct agatacagaa
    18661 ggctttaatt ccacgtatgc aactcaagct atacgggatc aggttcgtcc acagtacgac
    18721 gtgaacttcc acgacggatt tagctgggat acgcctcgtc aagcgcgttt aggtattctg
    18781 ttcaatttct agatggttgg atttgcttct tactcaagcc gggccatccg gcccgacttg
    18841 agtacgattt ttagatatta aaaatattat tgtaggagca tacaagtatg aataagagaa
    18901 aactaatatg gcttcttact tttgtatcag tggtgacggc cgtggtcgtt tcatcgctgt
    18961 acgacgtgag caacgccaaa gttgatacgg caacaaaact caaattacgt ttgaaaaaac
    19021 cggatattat tgatttggct tatgtcggat ataataactg gagttacgtt atgcgtaaca
    19081 acggttcttt tatgtatgat agccctgatc aagacggaaa tggcaataat gcgggcggag
    19141 aattcccccg tggttcaggc accaccatcg tatttgcggc tggagcctgg tttggcacga
    19201 tgaaaaataa tagagaagtt gtatctgaat gtcagtttgc ttctgaattt cagcccggac
    19261 gcattgaaaa ttcaggcgtt ccgtttgaag aacttacggc ggaagatttt ttaggaactg
    19321 aacgagaagt ttatcttatt gatcgctcag gcagcggtga tgattataat aattggccat
    19381 cggatgctcc ggtagatcaa ttcggattac ccggtcttat tgcagatgcg caaacctggg
    19441 ttgtatttaa tgacttggat acaaatcgta gccaagaagg tccagctgac tctccgtttc
    19501 ctggtttagg tattcagata acattagaat cctttgcatt caatgcaggt agcctttcta
    19561 atgtggtttt tttgaagttc acctttgata acaaaaccaa ttctgattac ccgaatagtt
    19621 atctcggttt atggatggat gccgatgtgg acaactcctc taacgacgtt attggttcgg
    19681 actcctctaa gggattggga tacgtgtata atgttgataa tacagatcaa ccgtatgcta
    19741 caggtttcga cttctttcag ggccctgtgg taagtgcgtc ggaagtatcg tcttttttag
    19801 cttttaaaaa cgcagataac aaaacagtat tgaaatacaa tcctgttcaa gggcgttatg
    19861 tacctactac attaggtggg gattcggtat ggcttggcgc tacgaccctc aacacttatc
    19921 ctaatagcgg cgatcccaat aacaatttag aacgttatag attacttcag gggattgata
    19981 gagatggtag cgtaaatccg aattcattta acggttggta ttatcccggg aatccgctta
    20041 ccggtattgt gaatgtacac actgtaggca acgaccaacg tattcttcac ggtgtaggcc
    20101 cgtttgtggt gaaagctggc gctaaacaag aagtctgggc tggcgttatc gccgggatcg
    20161 gcacggatcg cttaagtggc gttgcggaca tgttcaagac tgatgttttg gcacaaacga
    20221 cttttaatgc aggacttgta gctccggccc cgccccttac ccctaaaatt aatgtgacgg
    20281 gcttggatgg tcgcgtcaat attacgtggt cagacaatgc cgaactgaca tatgataatg
    20341 caggggatgt gctcggaatc aaagaagaca gcggttatac ggcggactat cttgccaatg
    20401 attttcaagg ttatcgtgtt tatcgcagtc taaccggact tcccggttcg tatgaacgtt
    20461 tagcccaata tgacaaggcc gatcatatta ccgtgatccg taactttact ctgaacacac
    20521 gcggttcgct tgatatccaa gatatcaatg taggtgatga tacgggattg cgttattttt
    20581 atgtagatga aaatgtcacc aatctcacaa cgtattatta ttctgtaaca gcttatgatg
    20641 cacagccata tatcgcagat gaggatgtgt tatttcatga tgccgattta ggcgtggata
    20701 ttcctaatcc gaccggctta ccgatcagct tggaaacttc cccgacatca aatgtggtca
    20761 gtgtagtccc gatggcggct acggcgggct cttcgtatga cgcttcggta acggccagcg
    20821 cgacgcatac cggtccaagc gatggatcgg ttgacttaga agttgttgat cgtgccgcag
    20881 ttacgggcca taattatcgc gttgagttct ttactattcc ggatagcgta gatggttatg
    20941 acctgatcca tcccgatcaa ggcgatatcg cttatcgggt gtatgatatt acagatggcg
    21001 tttataaaaa attctcaaca cgcgccgata atcctgatac ctaccttgat accaatgcta
    21061 atggggataa ggatgctacg gatttagtgt gggatgattc gtattatgcc accgcacaag
    21121 cttcagccgc agacgctacg accgaacaat tccacgttgt agatggtatc ttagtcaaaa
    21181 tttttggtcc ggctttggat gtcaaagact tcttgaccgt tgccaatgcc gcaggtcctc
    21241 tgaatccccc agtgtatgca gcttatgcat tcaatggtag cggattccca tctgcgcaac
    21301 ccggctttga tcctgtcaat ggtgtccttg atcgtccgac agacgatcaa caatctacca
    21361 ataataccaa gtggggtttc aacgtgggtg gtgccggtac caattttcat tacaatgatg
    21421 gtacgaccac ttcattcgtt gcgcgcgtat tccgaggaac caatttctca cgttttgttc
    21481 cttatgattt tgaattacga ttcacagcag cagggggtca aggtaatttc ttcttctcat
    21541 cgggaaatca gggcgctgta cctttcgaaa tttggaatgt aggtatcggc actcctgata
    21601 attcagcaga tgactatcgt atgtatccgc gtgtatttga tgaaggcggt gcggtcgatg
    21661 tatgggatat tacggagttt gatcacccaa tttcgggtgg tgataacgat ccttatacgg
    21721 actggattta ttggatgcgt cccacaaaca caaatcctgg tcaagccggc tacgatgcgg
    21781 ttgtaacggc aggcgatggt acaactggca ttaacgcaga agtcatggcg cgtgtgatac
    21841 ttgtaaactg gaatggtgga agtgtgtcag atccgtcata tcctgccaat ctagttgcaa
    21901 atcgtccaga aacggggacg gtatttcgta tcgtgtctac caaacccaat caaactacgg
    21961 atcagtttgc gtttagcacg acagctaata cggccctgac ttcaaagtcg gctaagaaaa
    22021 acagtttgga taaaattctt gtagtgccta acccgtacta tggtcgctcc acttatcaaa
    22081 aaacgctttt tgataaacaa gtgatgctta ctaatttacc tcccgtttgt acaatcaaaa
    22141 tatttaatgt agcgggcgac ctcattgcta ctcttaacca caatggggca agtaataaca
    22201 aacgcgttaa tacttcgccg ttgaatctcg cgacagaggc aagctctaaa acaaccggcg
    22261 ctgaagtttg ggatttacgt aatacgaatg gcgagtttgt ggcaagcggt atgtatatcg
    22321 ctgttgtgga agcctcaggt tatggaaaga aaatggttaa gtttgctgtt attcaggaaa
    22381 aaacaactat caatggtcct gatattcgct aggaatagaa cttgtcgtcc agcatcgaac
    22441 gtatggatga attaatgtag tttaaaatct tctgccggta aataacttac cggcagagga
    22501 aaacaagtca aggagaaatt atgaaatcaa aaatattagc attggcgatg gtattgcttt
    22561 atgcatcttc gacctttgcc ggtgaggaaa agcgcgggac cgcgggtttt atgtttctga
    22621 aagtgccgat gggcgcgcgc gaggtttcta tgggacagag cggtattaca acgtcgatgg
    22681 gtgctaacgc catttattgg aatcctgcta atgtttctat ggcaaccggg ccttcatttc
    22741 aagtctcgta ccttaaccat tttgccggca tttcgactaa ttatgccgcg ctatcatttc
    22801 ccatgtctga tattggtaca ttcggtttga gttttaatta tttaagttat ggcgacataa
    22861 caaaaacaga tgaattgcac ccggatggca atatcggtaa ttattcggct tatgatttgg
    22921 cttttggatt tacatactcc aagcaaataa cagatcgtgt taatggcggt atgacagtta
    22981 aattcctcaa ttcacacatt gatcaagtgg atgcttcagc gtatgctttt gattttggat
    23041 ttacttataa tacagatttc aaaggcctca aaatgagttt tgtggtatcc aatcttggtc
    23101 cacaatccac ctatagcggt gacggattac tacgtgaaac gaccgatcct ctaactggcg
    23161 ccactaccta ttttgcttat gaatccgaac cttttaacct tcctgcggcc gttaattttg
    23221 gtttgagtat ggatttgatg cgtaacgagc aaaattcagt gaccggcatg ctcgaacaaa
    23281 atgtgaatag ttttcaggta agtcgtacta attttggttt ggaatacgga tttcaaaaca
    23341 tgttctttgc gcgcgccggt tattcttccg cattgaaaaa agaccgtgat tacaataccg
    23401 gtaacgatgc tggtatgagc ggcttgacaa tgggtgcagg cgttgattac aaattcaacg
    23461 atagctttgg tgcaaccgta gattacggtt atttgaacat ggggctactt ggtggtactc
    23521 accgcttctc cgttggattg aaattctaat atttaaaaca ttagataaag cctcttctcg
    23581 ttatgggaag aggctttttt tttatacgtt tgtattgtat ttggttttgt gtatatagtc
    23641 ttttgttctc taaaatattg actttcagag tataaataac gagatttgcg cggaaatgaa
    23701 taaaatttgt tttaattaat tcaggtagag gcatagtatg tcagactacg tcaaatcggt
    23761 tttggcgcaa gttaaagcca aaaatccggc tgagccggaa tttcatcaag cagttcaaga
    23821 agtagccgaa tcattagaac cggtattgga tcgtcatccg gaatatcgta cagcaaaaat
    23881 attagagcgt atggttgaac cggaacgtgt agtaatgttt cgtgttccct ggcaagatga
    23941 tcagggtgaa atacatatca atcgcggttt ccgcgtgcaa atgaatagtg caatcggacc
    24001 atataaaggc ggtttacgtt ttcacccgtc cgtaaatctt ggaattttaa agtttttggc
    24061 ttttgaacag gttttcaaaa atagcttaac cacgttgccc atgggtggtg gaaaaggcgg
    24121 ttcagatttt gatcccaaag gaaaaagcga taacgaagtg atgcgctttt gccaaagttt
    24181 tatgacggag ctgtacagac atatcggcgc tgacctggat gtaccggcag gggatatcgg
    24241 cgtcggtggc cgcgaaatag gatttctttt tggtcaatat aagagactta aaaatgaatt
    24301 taccggtgtt ctcacgggta aaggtatcaa ctggggcggg tctttgattc gtccggaagc
    24361 taccggattt ggtgttacct attttgcaca agaaatgtta cgcaccaaag gtaaagacat
    24421 caaagggttg acctttgcgg tatcgggctt tggcaatgtg gcctggggcg ctgcacttaa
    24481 aatatccgaa ttgggtggaa aagtagttac tatttccggc cctgatggtt tcgtatatga
    24541 caaagacggc attcaaggtg aaaaaataaa ctacatgctc aaaatgcgtg caagtggcaa
    24601 agaccgcgtt aaagattatg ctgatgaatt caaagtagag tatcatgccg gtaaacgtcc
    24661 gtggagcgtc aaagtggatg tcgccgtacc ttgcgcaaca caaaatgagc tcggcgttga
    24721 agatgccaaa gagcttgtaa aaaacggatg tatctgcgtt tgcgaaggcg ctaatatgcc
    24781 gagcacgctg gatgcctacg aagtgttcaa acaagccggt attctgtttt cacccggcaa
    24841 agcttccaat gccggcggtg tagcaacatc cggtttggaa atgacacaaa acagtatgcg
    24901 gttgccgtgg tccgctgaag aggtggatca acgcctgcgt ggtatcatga agagcatcca
    24961 cgatacgtct ttgcatacgg caaaggaata cggtcacccg ggcaactatg ttgtcggagg
    25021 aaatattgcc ggattcttaa aagtcgctaa tgctatgatg gatcaaggtt tggtataaaa
    25081 gcgttttgaa attactaaca agaaaagagg ggttcaatcg aacccctctt ttctatttta
    25141 aatgataacc ataaaagttt gaagtttttt catcaattcg tcgtgacccg tgtaatgtat
    25201 agtctgccaa cctaagtcct ttgcgccatc tagatttttc gcaatatcat ctataaataa
    25261 aatttcttca gagcgaaggt tcataccggc aatggctttt tcaaagattt gtcgagcagg
    25321 tttcatcgca ccaacttcgt atgatagtat tagtctgtca aattctttgg taaaatcata
    25381 tgtgttgcgg atataatcga aatgaagtgc atttgtattg gaaatcaaac ctaaagtata
    25441 agttggtttc aaccgataaa gcagttcaat gttattatga atgggggtaa aaatttcagt
    25501 ccacaaaaaa tgaagttcat cggttgttcc tcgaaatctc aaactcaatt ttagtttttc
    25561 gaggaactct tttgaatcta attgcccgca ttcaaatgac atggctacgt cgctggctgt
    25621 gctacgataa atgatttcag tgattttgag ttcagacttg cgagaaatat gttcagccgc
    25681 agtattccaa tcaaaatcta caagtacttt tccaaggtca aaaaaaatag ctcgtatcat
    25741 ttaaaaccca acaattcacg ataggtggag tccgcattgg tatttttagt ccaataattt
    25801 gttttaatgg tgtgtgtcct gatgccaatg gagtgcagtt ttttagcttt ttcaccaaaa
    25861 ccatccatat agatcccttc aaacgaagta atctcgtgag gccacgattt tttgaaattg
    25921 atgatgagtg tacgattgta ggatgtatat tcgattttgt atgaaatgat cggacctgca
    25981 tgtccgcgat catcgggtaa ttcctgaagc gaggcctcag ccgtttccac acccagacga
    26041 cggtgtaaaa atcgagattg ggtaagtccc gggattattc gaatacggcc cgtaggcaaa
    26101 gcggtcggac caaggcgccc cttagtccac aatgcatcct cgagccaggc aggttcaaga
    26161 ggatacgatt catcaccttc tttttcgaag tatgaataac tctgtacgtc aaatttattg
    26221 ttacgatgat tgatttgagt atatacaaga ccgcaccact cttggacgga accggtgatt
    26281 ttgatagggt atcggtagcg agatgtatca agcggcataa atgcggaaac catcggtgaa
    26341 tagctgtata tgccggtatt aaagtttgaa acgaaattaa gcttcaaaat ggggattgaa
    26401 tgcgccttcg aatcgggtgt ataattttct aatttgactt gtttttcggt g
//
LOCUS       DAOQGF010000038        26134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_860_length_26134_cov_6.932896, whole genome shotgun sequence.
ACCESSION   DAOQGF010000038 DAOQGF010000000
VERSION     DAOQGF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_860_length_26134_cov_6.932896"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /gene="ppk1"
                     /locus_tag="PK937_07180"
     CDS             <1..1015
                     /gene="ppk1"
                     /locus_tag="PK937_07180"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNH32388.1"
                     /translation="AAEDPSVVAIKMTLYRIAANSPIAQALQLAAERGKEVAVLVELK
                     ARFDEEANIQWARKLEDAGAHVIYGMAGLKTHCKVCLIVRQEKNKIRRYCHLSTGNYN
                     ERTAQIYGDLGLFTAHPRFGEDLTNLFNVLTGYSAPPKFHRIKIAPTDLRDTFINKIR
                     REVNHVRDGKPGLLIAKMNALVDKQIILELYRASQAGVQIKCIIRGICCLKPGVKGLS
                     ENIEVISIIDRFLEHARIFYFHNNDQPEYWLASSDWMPRNLDQRVEVMFPILEPRHQQ
                     TVMTVLQTQLGDNVKARRFSADGTYVRVKNDRKKIRSQQVLYEIAGKLAKIYTVNESK
                     IFK"
     gene            complement(982..2382)
                     /gene="rsgA"
                     /locus_tag="PK937_07185"
     CDS             complement(982..2382)
                     /gene="rsgA"
                     /locus_tag="PK937_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH32389.1"
                     /translation="MSEPTNNDWSKKYLDDLNAKIKANKDVPKSPTDNSPKEQVKPQV
                     TPKDQPRHQQQQRDQQNNNKPQRDNQRKEPSREGNRNNPQDKRFFSDKPFKKNFQNNR
                     NDRDRKNDGRPNDPNRDRNEQKKNNNLPKKNEAPRKEDRRGGQQRPQDRDRRPESKPP
                     VVQKNRVLGGYICGMNGSVYTVLAENKEYTCTVQEYITRQGPLYIGDRVRIQIQPEMR
                     GVIEKYEPRQNFVLAPGARERQGDLVLAANVNQIVLVLSVMEPVLRTDWLDRHLLVCE
                     RKGFKPLIVCHKIDLADDNMFLEQMEVYKQMGYRVIFTSSAVLSSVNELKAAVKQKTS
                     LFTGHQGVGKTTLAELILEEESARLPDNPEEYDLTIVDDIVETTRQVHCLRGKEGIVL
                     IDTPGVTEYEISSIEKKDLKKYFRDFRNYNHQCSIPDCNHADEPNCKVKKAVETGEIS
                     EERYQNYLNILDSLTV"
     gene            complement(2391..2888)
                     /locus_tag="PK937_07190"
     CDS             complement(2391..2888)
                     /locus_tag="PK937_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNH32390.1"
                     /translation="MEHLWAPWRMRYIKAGDKNEGEGCIFCFKPQEDRDRENMILFRS
                     THNFVIMNLYPYNNGHLMVVPYQHAPDINALPPETLLDMMTTTQKCVTAFQRSVKPDG
                     FNIGFNLGRTAGAGIDDHVHMHVVPRWNGDTNFMPVIMDTKVMPEYLEQTYDRLLAEF
                     SQMKK"
     gene            complement(2915..>3856)
                     /locus_tag="PK937_07195"
     CDS             complement(2915..>3856)
                     /locus_tag="PK937_07195"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNH32391.1"
                     /translation="LYAPVRNYQDITAWKRMRETIEKDLLTVALMADCRHSTDMACAV
                     LPFTDGVRWTPDKAWTADVYEAHVRKLIESIAGFEKKILQIHFSPTEIPSFVDRHVQS
                     SSEKTGWMAIRVAQIAHEAGFSNLIISIQDSDFIAAHRILIAQLNEAGFNTPMVLHFE
                     HKANPLFDAASHIGSLLSDGIGDVVYLDCRAVASEETVRLAYNILQAARLRVSKTEYI
                     SCPSCGRTLFDLQEVTARIKSKTGHLKGVKIAIMGCIVNGPGEMADADFGYVGASAGK
                     INLYIGKELVERNIDMADADQKLIDLIRREGRWIEPA"
     assembly_gap    3857..3956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3957..5181)
                     /gene="ispG"
                     /locus_tag="PK937_07200"
     CDS             complement(<3957..5181)
                     /gene="ispG"
                     /locus_tag="PK937_07200"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNH32392.1"
                     /translation="MEPRTAIPQILNSSKLFRYSEYRFTYRRRPTREVMVGDIGIGGP
                     NPIRIQSMTTSDTLDTEATIRQVIDLVNVGCEIVRITAPSIKDAENLGAIREGLRKRG
                     INVPLVADIHFTPNAALKAAEYVEKIRINPGNYADKKKFAVIEYSENDYTRELERIEE
                     TFKPLVLKCKERGVAMRIGTNHGSLSDRIMNRYGDTPLGMVESALEFVRICEHYDYRD
                     IVLSMKSSNTQVMIAAYRLLAARMYELGMDYPFHLGVTEAGDGEDGRIKSALGIGTLL
                     EEGIGDTIRVSLTEDSIHEIPAARAIADKFNRQNVIFDTEKAALEIRLKEPRDPFQYH
                     RRWTQVVETAALNAGGVHHVRVALPLRSDLHDTMQAVNEIKQLSAHQTDNPFKAEILY
                     APVRNYQDITAWKRMR"
     gene            complement(5191..5937)
                     /locus_tag="PK937_07205"
     CDS             complement(5191..5937)
                     /locus_tag="PK937_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32393.1"
                     /translation="MSQEGLSREFKVNLIKSVYSKTAFDEVIAKANPYQLSDIQEFMW
                     DAALYIGRDEAGKPITREMITSQMMPTTEYWRGQLCGEPIDTCRGRSCFVSHPVCAGN
                     KLRAQIEVIRSCFDRRKQRSQQNEEGILMLLMYHINENHYAAAGLYDIQKHPIKTVGS
                     RVLSGDSLRNVIQAMNVAVGQGFTDSHMVQPVIIGEAKLMVEAKLIGSAHGDCVLALT
                     VPEDESSEKREKIFKKIEAGVRRITASAKA"
     gene            complement(5972..7006)
                     /locus_tag="PK937_07210"
     CDS             complement(5972..7006)
                     /locus_tag="PK937_07210"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNH32394.1"
                     /translation="MNTKEFMDTKEIIEVGVIGGGSFGTAMAIHLARNGYPVTIWAHD
                     AQAAEYMQTQRENKTYLPGVVLPNSLTATSDLQQTVRGKKLVLQVNPSHATRDIMQRA
                     VPFLEDDAIILNCTKGIENESLLTMSELLLQIIPSSKHSQLAFLSGPSFAREVALGAP
                     TAVSISSKNPSAAQYCQKIFASERFRVYTTDDIVGSELASALKNVIAIAAGISDGLGY
                     GHNTRAALITRGVAEILRLGKKMGAKEMTFLGLSGMGDLVLTCTGDLSRNRSVGIQLG
                     QGKKLEEILASMKMVAEGVKTAKAAYALAQKHGIEMPIIEQVYRVIFENKDPRAAVYD
                     LMTRESKSEA"
     gene            7149..7706
                     /locus_tag="PK937_07215"
     CDS             7149..7706
                     /locus_tag="PK937_07215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH32395.1"
                     /translation="MKIYLEAGVGFQSGTDAYGNEITGSSNTELTVKYLQEYLNNQSE
                     IRDRKTEVITTSNAATVLETMQPEDIFLTVHLNYYRQPQGVGLRGFYRSSRKLNSEKC
                     KALAITVTKALHDHTDVYYHGVFNEQRNNKPQLEPVLSTKATAALVEVGFNDDFKTKS
                     QSEMLHKNLAEGLGKGLVRHINAIR"
     gene            7934..9124
                     /locus_tag="PK937_07220"
     CDS             7934..9124
                     /locus_tag="PK937_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32396.1"
                     /translation="MLRKKLIWLSMLMATSAMVSAQIGNGLGTGRMGTFNTYSASVMG
                     AGFLSTYNQGRLFAVAGQSGFGTIKNVNLYHASNNFTAAYGFMDNFDALVSIITYQDI
                     NIRTVRGSQSLVPGDLYLSIRTGSYQIGEGSLEYGGSVTGRFPTGGQHNIPFEVYRSK
                     NIEFGVMGLLSYYSNPYYKDQSYSLNLNLGFWSHNDNGTVVTPSDDPNYSKAESDVNA
                     LALQYGLGLQIPVSKVHFMLDLYGLNYLTAPTKYVFSRESFAYSSFGLRYNLRSWLNV
                     GTFFDFQLAGKSDKTSYDGVGKLYGVTKPGQPANSSSKGAPNYNTWRFGFGLGFNILP
                     ATFGNSGGAVGGEQRRKKLLDRLMEEEKGAQRASNQLDKLKNIRVNAEKELEQIRLEL
                     EGGQ"
     gene            9137..9475
                     /locus_tag="PK937_07225"
     CDS             9137..9475
                     /locus_tag="PK937_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013571272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNH32397.1"
                     /translation="MKKIEAIIRPFKLDDVKEGLLQVGVKGMTISEVRGFGRQKGHTE
                     LYRGNEYRIDFLPKIKLEIVVENAMLDTTIDMIIDRAKTDQVGDGKIFVTNLEEVIRI
                     RTGESGEEAI"
     gene            9635..11056
                     /gene="glnA"
                     /locus_tag="PK937_07230"
     CDS             9635..11056
                     /gene="glnA"
                     /locus_tag="PK937_07230"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNH32398.1"
                     /translation="MSKEAIKKAMDLAKNRNVQVVDLKFMDFIGVWQHFTIPIYELND
                     EVWEQGLGFDGSSIRGWKAINESDMLVIPDPNTAMIDPFQQPTTLSIVCDVHEPLTKE
                     RFSRCPRGIAHAAEAYLKSTGIADTAYFGPEAEFFIFDSIRFDQTQNAGYYYIDSIEG
                     VWNRGRDENPNLGYKIRNKEGYFPVPPADQFQNMRNEMIMIMHECGLHVERQHHEVAT
                     AGQAEIDYKFNSLVRSGDDLMLFKYIVKNVAWKYGKTATFMPKPIFGDNGSGMHVHMS
                     LWKAGQPLFAGNQYAGLSELALYFIGGILKHTKALNALANPTTNSYKRLTPGFEAPVN
                     LAYSQSNRSAAVRIPMYSNSPKAKRVEYRCPDPSCNPYLAFAAMMMAGLDGILNKIHP
                     GNPLDKNIYDLAPDELAKVPSTCASLDEALRALENDHDFLLKGDVFTEDVIKSWISYK
                     YENEVNPMRLRPHPYEFALYYDM"
     gene            11178..12288
                     /gene="prfB"
                     /locus_tag="PK937_07235"
     CDS             join(11178..11252,11254..12288)
                     /gene="prfB"
                     /locus_tag="PK937_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121874281.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNH32399.1"
                     /translation="MIEISDIEKKLELAQFKISELRRFLDVDQRDQKIANLEAQTTVE
                     DFWNDNQRAQQIMQQIASERAWVDQWKKLDAQCEDLNMLLEIAEEENDEATIKSIDTE
                     LTELEKALARLELRAYFTNPEDNKSTVLTIHPGAGGTESQDWAEMLMRMYQRWAADRG
                     MKCQILDILDGDGAGIKSVTMEISGEFAFGLLKCESGVHRLVRISPFDSNARRHTSFA
                     SVYAYPEMEEVEAKIEINPADIKMDTYRSGGKGGQNVNKVETAVRLTHIPSGIVVACQ
                     VERSQHKNRDLAMKLLISKLYQMRKKEEQDKLDKLESAKSDIAWGNQIRSYVFHPYNL
                     VKDHRTDTETSNVQAVMDGDIDMFIEACLKQKVVQ"
     gene            12295..13992
                     /gene="argS"
                     /locus_tag="PK937_07240"
     CDS             12295..13992
                     /gene="argS"
                     /locus_tag="PK937_07240"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNH32400.1"
                     /translation="MKSYLEQQLHSALKALAIASDSEIIFTKTKDERFGNVATNLAMN
                     LAKTQKMNPRELAQKIVDHLQLDSSLVTKAEVAGGGFINFFFSPNYLFTQLKTILLQG
                     EEYGRHRFGANQRVDVEFVSANPTGPLSIGHGRQAVLGDVLCRLLSNAGYQVTREYYF
                     NNAGVQMKKLGESVRLRYLELLGETITFPEKHYEGEYIRDIAADIKSQHGESWKSYDF
                     EPFKSFAEDILFANIRKVLGRIGLKFDVYYNEDSLYKEGKIEALLKSFAAKNLTYVKE
                     NATWLKTSEFSYDHGPKPENDKVIVKSTGEPTYRLPDMAYHVTKFERGFDHIIDIFGA
                     DHIAEYPDVLAGLKALGYDTSHVRVLIHQFVTLMKEGEILKMSKRKANFVTIDELIDL
                     LGPDVFRYFLIMRSHNSHLNFDLELAQKQSMENPAYYIQNAHARICSIEKKAGERDIL
                     FSVSNEIKLDLLKADEETTIIEKLLQFPELTARLAKNLEPHPLTTYLEELAGVYHHYQ
                     TAGKKNPDLRVITEDKALSEARLALCIAVRIVLRRGLDILGIHAPEYMARDQADETV"
     gene            14064..14972
                     /locus_tag="PK937_07245"
     CDS             14064..14972
                     /locus_tag="PK937_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNH32401.1"
                     /translation="MDILVVGSVALDSVETPFGKVEDAVGGSATFFSAAASYFAPVNL
                     VAVVGNDYPMHAIEYLKSRRVDFKGLQIEQGETFRWAGRYNYDLNQRDTIYTHLNVFQ
                     NFKPHIPDAYKSSQYVFLGNIAPELQFDVLDQVQSPKLVALDTMNFWIEGSQKALRKV
                     LERIDIFILNDSEARQLANQPNLIKAAKEIFKMGPKAIVIKKGEYGAALITPDAQFNV
                     PAYPLEDVYDPTGAGDTFAGGFMGYIAKTDDASFENLKRAVVYGSAMASFCVQDFSIN
                     ALKELSQDKIFDRVLAFKQLSHFEEK"
     gene            14988..15455
                     /locus_tag="PK937_07250"
     CDS             14988..15455
                     /locus_tag="PK937_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNH32402.1"
                     /translation="MKLSEILNAKLIRIPLLQSNKQDIILEMLDVLVESGKISDREKV
                     LQSVLEREQMMSTGIGNSVAIPHGKSSGVEELQVALGITAQDINFDALDGKPVRIIFM
                     LVGPEKASSLHIKMLSRISRLLNQAPFRKKLTDAQSADEIMQIISDEEKLLEG"
     gene            complement(15452..15751)
                     /gene="yhbY"
                     /locus_tag="PK937_07255"
     CDS             complement(15452..15751)
                     /gene="yhbY"
                     /locus_tag="PK937_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNH32403.1"
                     /translation="MSELTGKQKRFLRGLGNAIKATISVGKSGITDNTYLSVNNGFNT
                     KELIKVKVQDGCDLDKEEVAEMISEGTQSQIVQIIGNNILLYKCHREKPIITLPD"
     gene            complement(15759..16334)
                     /locus_tag="PK937_07260"
     CDS             complement(15759..16334)
                     /locus_tag="PK937_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32404.1"
                     /translation="MQTESEYNASDAAVSNVKPDSASDSNKKYLFTGLWLIVIGTFWI
                     LWNMSVLSWGWMERYGLIIIGGVSIIKTIVTKKYYVFFGLTLLLIGVFHLYLDTQEGL
                     AMRTLWPVYFLISGIAFVINFFFNMRRWFSLVAGLSLIGFGGIHISRNFYMFPYDWVF
                     IIRTYWPLTFVASGIILVCAAWYKHRKLPND"
     gene            complement(16344..17018)
                     /locus_tag="PK937_07265"
     CDS             complement(16344..17018)
                     /locus_tag="PK937_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNH32405.1"
                     /translation="METTKQLFKSRRDKMVDGVCAGLAEYFNVNVALVRLLFVVFAFV
                     DCISVVAYITAMVLVPREPVSLTQDPDMELIEKRSAVNHGFFWGAILILIGAIMFLDT
                     MNLFNFHFFWRHFGWKFLVPTVFIIAGIFLIASRNRFAGSAGFFNKTSESTFRRASEN
                     KKIFGVCAGAAKHWNLDVSIVRIAWVLITIIAFPVGLIAYALVGLLTPDDKDQKVFGA
                     ASEKKL"
     gene            17124..18026
                     /locus_tag="PK937_07270"
     CDS             17124..18026
                     /locus_tag="PK937_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNH32406.1"
                     /translation="MNNILLLSICFILGIGLRKSGRFHEKTATVLNAFIIHIALPALT
                     LLHVHTLKISTDIWLPASMAWFFFAISAVTFYGLGHLLHWDRSTTGCMMLVGGLGNTS
                     FLGVPMIETFFGKEGIPIGLVVDQLGSFLVISTLGIWTVNYYQGQSVSLKMLIKRIIV
                     FPPFIALMLAVALIPYEFPAPLTYTLDRLGQTLAPLALFSVGFQIHFSKINSLRIPLV
                     LGLSFKLLIAPAMLFILYSTLTADRYLLFDITIFEAAMPPMITAAILATEHDLRPELA
                     SLMVGIGIIVSMFTLPFWWVLLTL"
     gene            18085..18561
                     /locus_tag="PK937_07275"
     CDS             18085..18561
                     /locus_tag="PK937_07275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNH32407.1"
                     /translation="MPDTIIVLETTQGTIEVKLFPDIAPKASENFEQLVEKGYYDGII
                     FHRIIKDFMIQGGDPTGTGRGGQSVWGMPFDDEFSFKVQFDKPGILAMANAGPRTNGS
                     QFFITTVPTPWLNNRHSIFGEVISGYDVVKKLEGVKTNPMDKPLQDQKIIKAYKKA"
     gene            18569..19528
                     /locus_tag="PK937_07280"
     CDS             18569..19528
                     /locus_tag="PK937_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNH32408.1"
                     /translation="MMPYRILLYYKFTEISDYEKFAAEHLTLCQSLGLLGRIIVAPEG
                     INGTVSGTVEQTDIYQKTILSDPRFSDMVIKSDPSDKHAFRRLSVKARTEIVTLGPDN
                     VNPTEKTGHYLEPPDFYAAMKEPDVVIIDARNDYEYDMGHFRGAIRPDVSNFKEFPAW
                     VRQNLSSYRDKKVLTYCTGGIRCEKFTGLLINEGFKDVYQLHGGIVMYGKHPDTKGRD
                     FEGKMYVFDERIGVEVNHTETASIITRCLYCETVTDHFVNCAHLDCHKSFYICAACDR
                     ANQRSCSEACKNADHHEYRVLGDALFEAPLTPRGLFKSILPTS"
     gene            19610..20557
                     /locus_tag="PK937_07285"
     CDS             19610..20557
                     /locus_tag="PK937_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HNH32409.1"
                     /translation="MDTVKHLGELAIGSRLRRLSDRLLANVNRIYQEHGINFETRWFL
                     MFYQLHLKSPMSITEIAAATGFTHPAIIQLSDELIKVGLIASVTDTTDKRRRLISLSA
                     KGIEMLGHLKPVWHQIEEAHRQISREIGMDLLSTLERYEEILDERDIYERVTHATKVS
                     SASDIVIHTYAPRFKKYFKLLNIEWLKKFFTIEPLDRKILSHPETIIKDGGEIFFAEW
                     NGKIVGTCAVLNNKAGIYELAKMAVTEKAQGKHIGWKLGSHAITWAQKNKGKTMVLET
                     SRKLYAALALYRKLGFVLVANPEPSKYERSTIKMELRLR"
     gene            complement(20560..21456)
                     /locus_tag="PK937_07290"
     CDS             complement(20560..21456)
                     /locus_tag="PK937_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNH32410.1"
                     /translation="MTDIKKYLQPDVVARLANLEMIARFVVEGFITGLHKSPYHGFSV
                     EFAEHRQYMPGDEIRSIDWKVYGRTSRYYVKRFEEETNLKCHIVLDTSASMTFGTGEV
                     NKLQYGGYLAASLAYLMIHQKDAAGLAIFDKVLRKYLPPRATLGYIKTIEEALQNISH
                     HETTRIAENLHAVAERIKRRGLVILISDLLDEAEDVVHALRHFRHDGHEVIVFHVLDH
                     AERTLDFEGDVVFDDLETGEQLATQPWHIRKAYTQEVESFCDYYRRRCRESRVDYVLL
                     DTKTPFDVALREYIAKRSKIGG"
     gene            complement(21513..24893)
                     /locus_tag="PK937_07295"
     CDS             complement(21513..24893)
                     /locus_tag="PK937_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32411.1"
                     /translation="MRSFFRLPVGFIMLTVYLLTGKVAAQEEQKWFGQNKVQYQEFNW
                     RYIQSKHFDIYYYKGGEDIARFTAEVAESAYVHIKECFRNYDIQNRIALVVYNSHNQF
                     QQTNVLDEYMSEGIGGVTELFKNRVVIPFEGSYEQFRHVIHHELVHGVMNDYMYGGSI
                     QGIISGRIRVQVPLWVAEGLAEYSSRYGAFNTQSDMFVRDAVMEAYLPPLNQMGGYAV
                     YTAGPTVFRYMEEKYGREKVAEFMTKLRVAGTPNATFESTFGMKEEEFSDKWATYQRK
                     IYYPDISQMVSVKEIGKALTNHLKDENFYNMTPTISPNGDKIAYLTDKSGYADIMLIS
                     AYDGMPLKKLVSGEKTPNLEELHWLSPGMSWSPDSKKLVFAAKASDNDALLVVDVMTG
                     DITKYSWPDLEGVFGGSWSPDGKKIVFSGMRFGQSDVFEFELASGKLSKLTDDVFSDS
                     RAVYSRDGSKIAFVSDRRDILGKQPDGFKMINFEYRQTDIYVMNADGSGMERITTDEM
                     NDSWPEWGPDNSKLLFTSDRNGVSNIYVADLKEKKNYAITNNLSGIFQPSLSKDGKFL
                     TFSGFNRSGFDVYTLKNPFEMKPVELPITISMKKFRKDHDLPETLADEIGIKKQQNIV
                     SSNEGESSTVTDPKADSLKTQESKVSYRNFVFAGLEDENNNSSVKDEDEPEEAVELPE
                     NVYKNEKGDYRVRRYRVKFSPDMVYGTAGFNTLTGFQGVTQFAFSDMLGDHRLLLGTN
                     LFFDLKNSSFTGSYYYLANRTDYGITAFHSAYAFATDYLDERIDINGNPVFFNGQDGY
                     PDSFVRFRFYGLGLNASRPINKFNRIDFSATQLVLARETSWNARNDSLPPKNSTGLGR
                     SRQSNNTVLTLSLINDNSLNTYFGPFDGQRAQVAITASPGYGQNGLRFVTATVDGRKY
                     FWFKKFYAIATRASFGATFGRNPQRFFVGGLDNWILPRFNNGDILVDTFEDFLATYVS
                     PLRGSNYYELQGTRYFISNFEFRFPLVHFMQLGFPIPMFLQQIRGVTFVDFGAAWGGE
                     DREFVVDQLTGETKKANYRYIDRNGNIVYNKSRFDWGQTLKGEGNPVFQDARIGYGFG
                     IRAFVGFFILRYDLAWNIRNPRFSGGNAKHYFSIGSDF"
     gene            25095..25520
                     /gene="ndk"
                     /locus_tag="PK937_07300"
     CDS             25095..25520
                     /gene="ndk"
                     /locus_tag="PK937_07300"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNH32412.1"
                     /translation="MSRHTLAILKPDCVRNNQQGAVIAEIQKAGFKIIAMKQTRLTKA
                     QAEGFYEVHRQRPFFGELTEFMSSGPCVPMVLEKDNAVAAFRTTIGATDPKEAAEGTI
                     RKLFASSKAENVIHGSDSDENAKNEIAYFFAHSELIAIR"
     gene            25600..>26134
                     /locus_tag="PK937_07305"
     CDS             25600..>26134
                     /locus_tag="PK937_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNH32413.1"
                     /translation="MNAEILVIGDEILLGLITDTNSAYISRRLAQYGVAVTRITKVGD
                     QTEIIADALRASSSRASLVISCGGLGPTHDDKTRDAAALFLNVPLQLNESALKEIETL
                     YVKAGRTMSAANRVQAMIPEGAMYLSNERGTAPGLHFIKGDTAFFCFQGVPKEMEWMT
                     ENYLIPFVQKKPSSHVVR"
BASE COUNT         7452 a         5925 c         5405 g         7252 t
ORIGIN      
        1 agcggcagaa gacccgagtg tagtggcgat taaaatgacg ctttatcgta ttgcagccaa
       61 ttccccgatc gcgcaagcct tgcagttagc tgccgagcgc ggcaaagaag tcgccgtgtt
      121 ggtcgagctc aaagcacgtt ttgatgaaga ggccaatatc caatgggcac gcaagcttga
      181 agacgccggt gcgcatgtga tttatggtat ggccggactc aaaacacatt gtaaagtatg
      241 ccttatcgtg cgtcaggaaa aaaacaaaat ccgtcgttat tgtcatttat cgacaggaaa
      301 ctataatgaa cgcacggcgc aaatatacgg tgatctgggc ttatttacgg cgcatccgcg
      361 ttttggtgaa gacttgacca atttgtttaa tgttttgacc ggatactctg ccccgccaaa
      421 atttcatcgc atcaaaattg ctccgacgga tttgcgcgat acatttatca ataaaatccg
      481 acgtgaagtc aatcacgtgc gtgatggtaa acccggcctt ctcattgcca aaatgaatgc
      541 cttggtggat aaacagatta ttttggaatt gtatcgtgca agccaggccg gtgttcagat
      601 caaatgcata atacgcggta tttgttgttt gaaacccggc gtaaaaggac tgtcagaaaa
      661 cattgaagta atcagtatca ttgatcgttt tcttgagcac gcacgtattt tttattttca
      721 caacaatgac caacctgaat attggctcgc cagttcggac tggatgccgc gtaatttgga
      781 ccaacgcgtc gaagtcatgt ttcctatact ggaaccgcgc catcagcaaa cggtgatgac
      841 cgtactccaa acgcagctcg gcgataatgt aaaagcccgc cggttttcag ccgacgggac
      901 ttatgtacga gtcaaaaatg atcgtaaaaa aattcgttcg cagcaggtac tgtacgaaat
      961 agctggtaag ttagccaaaa tttacaccgt caacgaatcc aaaatattca aataattttg
     1021 atagcgctct tctgaaatct ctccggtttc aacagccttt ttgactttac aattcggttc
     1081 atccgcatga ttacaatcgg gaatagaaca ttggtgattg taatttcgga aatcacggaa
     1141 gtatttttta agatcttttt tctcaatact cgaaatctca tattcggtaa cgcccggcgt
     1201 atcaatgagg acaattcctt ccttcccgcg cagacaatgt acttgacggg tggtttccac
     1261 aatatcatcc acgatcgtaa ggtcatactc ttcgggatta tccggcaatc gagcgctttc
     1321 ttcttcgagt ataagctccg ccagcgtagt tttcccgact ccttgatgac cggtaaataa
     1381 cgaagtcttt tgttttacgg cagcttttag ctcattcaca ctggagagca cagcggacga
     1441 ggtaaatata acgcgatatc ccatctgttt atagacttcc atctgctcta gaaacatatt
     1501 atcatcggcc aaatcaattt tgtggcatac gatcagtggt ttgaaacctt tacgctcgca
     1561 caccagcaaa tgccgatcca gccaatccgt acgcaacacc ggttccatta ccgacaggac
     1621 taaaacgatt tgattgacat tagctgccag taccaaatca ccttggcgtt cgcgtgcacc
     1681 gggagctaac acaaaatttt gacgcggttc atatttctcg atcacgccgc gcatctcagg
     1741 ctggatttga attcggacac gatcgccgat atataacgga ccttgacgtg tgatatactc
     1801 ctgaaccgta catgtatatt ctttattttc cgctaaaaca gtatacactg agccgttcat
     1861 tccgcagata tatccgccaa gaactcggtt tttctgtaca accggcggtt tagattccgg
     1921 tctgcgatcg cgatcttggg gacgttgctg accgccgcgg cgatcctctt tacgaggcgc
     1981 ttcgtttttc tttggtaaat tattattctt tttctgctca ttacgatcgc gattcggatc
     2041 attgggccga ccatcgtttt tgcgatcacg atcattgcgg ttattctgaa aattcttttt
     2101 aaaaggtttg tcagaaaaaa atcgtttgtc ctgcgggtta ttacgattgc cttcgcgtga
     2161 aggctctttg cgttgattat cgcgttgcgg cttattgtta ttctgctgat cgcgctgctg
     2221 ttgctgatgg cgaggttgat ctttaggagt aacctgaggt tttacttgct ctttcgggct
     2281 attatccgta ggcgatttgg gtacatcttt gttggcctta atcttggcat taagatcatc
     2341 cagatatttt ttggaccaat cattgttcgt cggttcagac ataaaactcc ttacttcttc
     2401 atctgcgaaa attcagccaa cagacggtca tacgtctgct ctaagtattc aggcatcact
     2461 ttggtgtcca tgattaccgg cataaaattg gtatcgccgt tccatcgcgg cacgacgtgc
     2521 atgtggacgt ggtcgtcaat gcccgctccg gcagttcgtc cgagattaaa tccgatattg
     2581 aaaccatccg gtttgacgct gcgctgaaat gctgtgacgc atttttgcgt ggtcgtcatc
     2641 atgtccaata gtgtttcggg cggcaaagca ttgatgtccg gagcatgctg gtacggcacc
     2701 accatcaaat gaccgttgtt gtacggatat aagttcatta ttacaaaatt atgcgtcgag
     2761 cggaaaagga tcatgttttc acggtcccga tcttcttgcg gtttaaaaca aaagatacat
     2821 ccttcgcctt catttttgtc gccggctttg atataccgca tgcgccaggg tgcccataaa
     2881 tgttccacta cgttactcca tcctaagtat aaaattatgc cggttcgatc catcgccctt
     2941 cgcggcgaat aagatcaata agtttttgat cggcatcggc catatcaata ttacgctcga
     3001 ctaattcttt tccaatatac agattgatct tcccggcgct ggctccgaca tatccgaaat
     3061 cagcatcggc catttcgccc ggtccgttga cgatacaacc cataatagcg attttaacgc
     3121 ctttcaaatg accggtcttg gatttgatac gtgcggtaac ttcctgcaaa tcaaacaacg
     3181 ttcggccaca cgacgggcac gaaatgtatt ccgttttgga aacacgtaag cgtgccgctt
     3241 gcaaaatgtt atacgctaag cgtacagtct cctccgaagc cacagcccgg caatctaaat
     3301 aaacaacatc gccaatacca tcggaaagca gagaaccgat gtgcgaagcg gcatcaaata
     3361 agggattcgc tttatgttca aaatgcaaaa ccatcggcgt attaaaaccg gcttcattca
     3421 gctgagcgat cagtattcga tgcgcagcaa taaaatcact atcctgaatc gaaataatca
     3481 aattactaaa tcccgcttcg tgcgctatct gagcaacgcg gatcgccatc caacctgttt
     3541 tctccgatga tgattggaca tgacgatcaa cgaaagaagg tatctcagtc ggtgaaaaat
     3601 gaatctgcaa aatttttttc tcgaatccgg ctattgattc gataagttta cgaacatgcg
     3661 cttcgtacac atcggccgtc cacgccttat ccggagtcca acgtacgcca tcagtaaaag
     3721 gaagtacagc acaagccata tctgtcgaat gcctacaatc cgccatcaaa gctacagtta
     3781 ataaatcctt ttcaatcgtt tcacgcatgc gtttccatgc cgtgatatcc tgataattgc
     3841 gtaccggtgc gtacaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacg
     3961 catgcgtttc catgccgtga tatcctgata attgcgtacc ggtgcgtaca aaatctcggc
     4021 tttaaaggga ttatcggtct ggtgagcgga aagctgtttg atttcattta cagcctgcat
     4081 cgtatcatgg agatcgctcc gcagcgggag agcaacacgc acatgatgca cgccgccggc
     4141 attaagcgcc gctgtttcaa cgacttgcgt ccatcttcga tggtattgaa acgggtcccg
     4201 cggttcttta agacgaattt ctaaggcagc tttttctgta tcaaaaatta cgttctgacg
     4261 attaaattta tccgctatag cgcgggcggc cggtatttcg tgtattgaat cttcggttaa
     4321 cgaaacacgt atcgtgtcac cgataccctc ttccaataat gtgcctatgc ctagcgctga
     4381 tttgatacga ccgtcttcgc cgtcgccggc ttccgttacg ccaagatgaa aagggtaatc
     4441 catgcccaat tcatacatcc gcgcggcaag cagacggtac gcggcgatca tcacctgcgt
     4501 attcgacgat ttcatggaaa gaacaatatc gcgataatcg tagtgttcgc aaattctaac
     4561 aaattcgagc gcggactcca ccataccaag cggggtatcg ccatagcgat tcattattcg
     4621 atcggataac gaaccgtgat tggtgccgat acgcattgcg acaccgcgtt ctttgcattt
     4681 gagaaccaac ggtttgaagg tttcttcgat acgttcaagt tcacgagtat aatcattttc
     4741 ggaatattcg atcacggcaa atttcttctt gtccgcgtag ttaccgggat ttatacgtat
     4801 tttttctaca tactcggcgg cttttaaagc cgcattagga gtaaagtgaa tgtccgcaac
     4861 caacgggaca ttgataccgc gtttacgcag tccttcgcgt atcgcaccca gattttcggc
     4921 atctttgatc gatggcgcgg taatgcgaac gatttcacag cctacattca caagatcaat
     4981 gacttgccgg attgtcgctt cggtatccaa tgtatccgaa gtcgtcatgg attggattcg
     5041 aatgggattg ggaccaccga ttccgatatc gccgaccatg acctcgcggg taggtcgccg
     5101 acgataggta aaccggtatt cactatagcg aaagagttta cttgagttca atatttgcgg
     5161 gatagccgtc cgaggttcca tgccaaataa ttacgccttt gcgctcgccg taatacgccg
     5221 gacgcccgcc tcgatttttt tgaaaatttt ttctcgtttt tccgaacttt cgtcttccgg
     5281 gacagtcaaa gccaacacgc agtcgccatg tgcgctaccg atgagtttgg cctcgaccat
     5341 aagtttggcc tcaccaatga taacaggctg aaccatatgg gaatcggtaa atccctgccc
     5401 tacagctaca ttcattgctt ggatgacatt acgaagcgaa tcgcccgaca acacacggga
     5461 cccgactgtt ttgattggat gcttttgaat gtcatacaat ccggcggccg cataatgatt
     5521 ttcattaata tgatacatca gtagcatcag aattccttct tcattttgtt gcgagcgctg
     5581 tttacggcga tcaaaacaac tgcggataac ttcaatctga gcgcgtagtt tattgcctgc
     5641 acataccgga tgtgaaacaa agcatgatcg cccgcgacaa gtatcaatgg gttcaccgca
     5701 aagttggccg cgccaatact ccgtggtcgg catcatttgc gatgtgatca tttcgcgtgt
     5761 tattggctta cccgcttcgt cacgcccgat gtaaagcgct gcatcccaca taaactcctg
     5821 aatgtcagat aattgatacg ggttagcctt ggcgataacc tcatcaaagg ctgttttact
     5881 ataaactgat tttatcagat ttaccttaaa ttcgcgtgat aaaccttcct gtgacatatt
     5941 gatttcctta tttctgcctc gttaaagagg ttcaggcttc cgatttggat tcgcgtgtca
     6001 tcagatcata taccgctgcg cgcggatctt tattttcaaa tataacgcgg tacacctgct
     6061 ctatgatggg catttctatg ccgtgttttt gtgctaacgc ataagccgct ttggctgtct
     6121 taacgccttc ggcaaccatt ttcatggagg ctaaaatttc ttcgagtttt tttccttgtc
     6181 ctaattgaat gccgacgctg cgatttcgac tcagatcacc ggtgcatgtg agaaccaagt
     6241 ctcccatacc cgaaagaccc aaaaaagtca tctctttggc tcccattttt ttcccaagac
     6301 gcaatatctc cgcaacacct cgcgttatga gcgccgcacg agtattgtgc ccataaccaa
     6361 gcccatcgct gatacccgcc gcaatggcga taacattctt cagcgcactc gccagttcag
     6421 atccgacaat atcatccgtc gtgtatacac gaaaccgttc actggcaaaa attttctgac
     6481 aatattgcgc cgcggaagga ttcttagaag aaatcgaaac agccgtcggc gctccgagcg
     6541 ccacttcccg cgcaaaactc ggtcccgata aaaaggccag ttgactgtgt tttgaagacg
     6601 gaataatctg aagcaataat tctgacatgg ttaacaaaga ttcattttca attcctttgg
     6661 tacaattgag aatgatcgca tcgtcttcca aaaaaggaac ggcacgttgc atgatgtctc
     6721 tcgttgcatg ggatggatta acctgcaaaa ccagcttttt accacgcaca gtttgctgta
     6781 gatcagatgt agcggtcagg ctattcggta aaacgacacc cggtaaatag gttttgtttt
     6841 cccgttgggt ctgcatgtat tcagcggctt gtgcatcatg cgcccaaatt gtaacagggt
     6901 aaccgttacg tgccaaatga atagccatgg ctgtcccaaa acttccaccg ccaatcacgc
     6961 cgacttctat tatttctttt gtatccataa attcttttgt attcataaag aaaatatagt
     7021 atacgttgct tattatatca agacttatta aaacacaaat tttacaataa gttagtttta
     7081 acttgaattt ttattgtatc ggtaatactt ttcccttgtt caagcacttt tcttaggaga
     7141 aatattcgat gaaaatttat ctcgaagccg gagtcggctt ccaatccgga acagacgcgt
     7201 atggtaatga aattaccggt tcatctaaca cagagttaac cgtaaaatat ttacaagaat
     7261 atttaaataa ccaatctgaa atacgggatc gtaaaacgga agtaataacc acctccaacg
     7321 ccgccaccgt acttgaaacg atgcaaccgg aagatatttt tttaacggta catctaaatt
     7381 actacagaca gccccagggt gtgggcttac gcgggtttta tcgaagctcc cgtaagctca
     7441 attccgaaaa gtgcaaagcg ctggctatta cagttaccaa agcattgcat gatcacacgg
     7501 atgtatacta tcacggtgtc ttcaatgagc aacgtaataa caagcctcag ctcgaaccgg
     7561 ttttgtctac taaagctaca gccgctttgg tagaagtcgg ttttaacgat gattttaaaa
     7621 caaaatcaca gtctgaaatg ttacataaaa atttagccga aggattaggt aaaggtttag
     7681 ttcgtcacat aaacgctata cgctaaaagt gaccattaag accaaaaaac tgtattattt
     7741 tattggtata attacaattg aataggtcac tttcccttga aaaaagatga cttaaagttc
     7801 attttaataa atacttgaca ttgcaccaat aatcatctat tttttctcaa aataccgtca
     7861 tattgcttaa tttaagaatt atacagatta atattaaaat ttatttataa acatccgaaa
     7921 ttcggaggaa tgtatgttgc gtaaaaaatt gatttggctg agtatgttaa tggcaacttc
     7981 agccatggtt tccgcgcaga tcggcaatgg gcttggaacc gggcgtatgg gtacgtttaa
     8041 cacctattcc gcatcagtta tgggtgccgg tttcttgagt acttacaatc agggacgttt
     8101 attcgcagtg gcaggacaga gcggatttgg aacaatcaaa aatgttaatt tatatcatgc
     8161 gtccaacaat ttcacagcag catacggatt catggataat tttgatgctt tagtttctat
     8221 tattacatat caagacatca atattcgtac tgttcgcggt tcgcagtcat tggtaccagg
     8281 cgatttatat ctctcgattc gaactggaag ttatcaaatt ggtgaaggta gtttggaata
     8341 tggcggatcc gtcacaggac gttttccaac cggaggacag cataatattc cttttgaagt
     8401 atatcgttca aagaatattg aattcggtgt aatgggatta ttatcgtact atagtaatcc
     8461 atattacaag gatcagagct atagtcttaa tttaaacttg ggattttgga gtcacaacga
     8521 taatggtact gtagttacgc catcggatga tccaaattat tcaaaagctg aaagtgacgt
     8581 caatgctctt gcacttcaat atggattagg tttacaaatc cccgttagta aagtacattt
     8641 tatgttggat ttgtatggcc taaattacct caccgctcct accaaatatg tatttagtcg
     8701 tgaatcgttt gcttattctt ctttcggttt acgctacaat cttcgctcat ggttaaatgt
     8761 tggaactttc tttgacttcc aattagccgg aaaatctgac aagacgagtt atgatggggt
     8821 tggcaaatta tatggcgtta ccaaaccagg tcaacctgca aattcttcct caaaaggtgc
     8881 acccaactac aatacttggc ggtttggctt cggacttggg tttaatatcc ttcctgccac
     8941 ctttggtaat tcaggtggag cggttggtgg tgaacaacgc cgtaaaaagc ttttggatcg
     9001 ccttatggaa gaagaaaaag gcgctcaacg cgcatccaat caacttgata aactgaaaaa
     9061 catccgggtt aatgccgaaa aagaacttga gcaaattcgt ttagaattgg aaggcggaca
     9121 gtagtatata caggaaatga aaaaaatcga agcaatcatt cgaccattta aacttgatga
     9181 cgtcaaagaa ggacttcttc aagtcggtgt aaaaggaatg acgatttccg aagttcgagg
     9241 atttggaaga caaaaaggac acacagagtt atatcgaggt aatgaatatc gcattgattt
     9301 tttaccaaaa ataaaacttg aaattgtagt tgaaaatgct atgttagaca caaccattga
     9361 catgattata gatcgggcta aaactgatca ggtcggcgat ggtaagattt ttgtaaccaa
     9421 tttagaggaa gtgattcgga ttcgtaccgg agaatcaggt gaagaagcga tctaacatac
     9481 gagccgttcg acggcgaaca cgagacatcc gaagtgtctc tatcgccaaa aaaagtacat
     9541 tataggaatt ttagcccctg accaaaaatc aggggctttt gattttaatt aacttaagta
     9601 agcaacaatt cacttatcaa ccaaggagct atcgatgagc aaagaagcca tcaaaaaggc
     9661 tatggacctt gctaaaaacc gcaatgttca agtcgttgat ctcaagttta tggattttat
     9721 tggtgtgtgg cagcatttca ccatccctat ttatgagctg aacgatgaag tttgggaaca
     9781 aggtctcggt tttgacggtt ccagtattcg cggctggaaa gctatcaatg aaagtgatat
     9841 gcttgttatc cccgatccga atacagccat gatcgaccct tttcagcagc cgacaacatt
     9901 gagtattgtg tgcgatgtgc acgaaccgct gaccaaagag cgattcagtc gttgtcctcg
     9961 cggtatcgct catgctgccg aagcttatct caaatccacc ggaatcgccg atacggctta
    10021 ctttggtccg gaagccgaat tttttatttt cgacagtatc cgatttgatc agacacaaaa
    10081 cgccggatat tattacattg actcaattga aggtgtatgg aatcgtggcc gcgatgaaaa
    10141 tcccaattta ggctacaaga ttagaaacaa agaaggttac ttccccgttc ccccggctga
    10201 tcaatttcaa aatatgcgta atgaaatgat aatgatcatg cacgaatgcg ggcttcacgt
    10261 cgaacgtcaa caccacgaag tcgcaacggc cggtcaagca gaaattgatt acaaatttaa
    10321 cagtctcgtc cgctccggtg acgatcttat gcttttcaaa tatatcgtta aaaatgttgc
    10381 atggaaatac ggtaaaacgg ctacgtttat gccaaaacct attttcggcg ataacgggtc
    10441 gggcatgcac gttcacatga gcctctggaa agcagggcaa cctctttttg ccggcaatca
    10501 atatgcagga ctgagcgagc ttgcactgta ctttattggc ggaattttaa aacatacaaa
    10561 agcgctcaac gccttggcaa atcctacgac gaactcctac aagcgcctta caccgggatt
    10621 tgaagcgccg gtcaatctcg cctattcgca aagtaatcgt tcggcggctg tacgcatacc
    10681 gatgtattcc aatagcccta aagcgaaacg tgtagaatac cgttgccccg atccgagttg
    10741 taatccctat cttgcgtttg cagccatgat gatggccggt cttgatggta ttctcaataa
    10801 aattcatcca ggtaaccctc tggataaaaa catctacgac cttgctccgg acgaattggc
    10861 caaagtacct tcgacttgcg catcgttgga tgaagctttg cgagcattag aaaacgatca
    10921 tgacttcctt ctcaaaggcg atgtcttcac tgaagatgtg atcaaatctt ggatcagcta
    10981 taagtatgaa aacgaagtga accccatgcg tctgcgcccg catccgtatg aatttgcttt
    11041 gtactacgat atgtgatcgt ttgaactcta ctctgttaaa aggccggtgc acacgcatcg
    11101 gcttttttat ttttgacttt tctgagtgaa ttggttaatt tcgcacgcat ttttttaacc
    11161 ataggaacca tactgccgtg attgaaattt ccgacatcga aaaaaaactc gaactcgctc
    11221 aatttaaaat ttcagaattg cggaggtttc tttgacgtcg atcaacgtga tcaaaaaata
    11281 gccaacctcg aagcgcagac taccgttgaa gatttttgga atgacaatca gcgtgcgcaa
    11341 cagatcatgc aacagatagc ctctgagcgc gcatgggtag atcaatggaa aaagctggat
    11401 gcgcaatgcg aagatctgaa tatgctttta gagatcgctg aagaggaaaa tgacgaagct
    11461 acaatcaagt ccattgatac cgaactgact gagcttgaaa aggcgcttgc acgcctggag
    11521 ttacgcgctt atttcacgaa tccggaagat aacaaaagca ctgttcttac gattcaccct
    11581 ggtgccggcg gaacagaatc ccaggattgg gcagagatgc ttatgcgtat gtaccaacgc
    11641 tgggccgccg atcgcggcat gaagtgccag attctcgata ttcttgacgg cgatggtgca
    11701 ggtattaaga gcgtgacgat ggaaatttcc ggtgaatttg catttggtct gcttaaatgt
    11761 gagagtggcg tacaccgttt ggtgcggata tccccttttg actccaatgc tcgccgtcac
    11821 acgtcatttg cgtccgtata cgcctatccg gaaatggaag aagtcgaagc caagattgaa
    11881 atcaatccag cggatatcaa aatggatacg tatcgctccg gaggtaaagg tggtcagaat
    11941 gtaaacaaag tagaaacagc cgtgcggttg acacatattc cttccggcat cgtcgtggca
    12001 tgtcaggtag aacgttcaca gcataaaaac cgcgatctcg ccatgaagct tctgatctcc
    12061 aagctttatc aaatgcgtaa aaaagaagaa caagataaat tagacaaact tgagagcgcc
    12121 aaatccgata tcgcatgggg caatcaaatc cgttcttatg ttttccatcc gtataatctc
    12181 gtaaaagatc accgcaccga taccgagaca agcaacgtgc aggctgtcat ggatggtgat
    12241 attgatatgt ttatcgaagc ctgcctcaaa caaaaagtcg ttcaataata agtcatgaaa
    12301 agttacctcg aacaacagct tcactctgct ctaaaagcac tagccatagc gtccgatagt
    12361 gaaataattt ttactaaaac aaaggacgaa cgttttggta atgtggcgac taatttggca
    12421 atgaatctcg ccaaaactca aaaaatgaat cctcgcgaat tggcacaaaa aattgttgac
    12481 catctccaac ttgattcatc cttggtcacc aaagcggaag tcgccggtgg cggatttatc
    12541 aatttctttt tcagtcccaa ttaccttttt actcagctta aaactatact gctgcaaggt
    12601 gaagaatatg gtcgtcatcg ttttggggcg aatcaacggg tggatgtcga atttgtcagt
    12661 gcaaatccta caggtccttt atctataggt cacgggcgtc aagcggtgct cggcgatgtt
    12721 ctttgccgat tattatccaa tgccggatat caagtaacac gcgaatatta ttttaataat
    12781 gccggcgttc agatgaaaaa attaggtgaa tccgtacgcc tacggtattt ggaattgttg
    12841 ggtgagacca tcacttttcc tgaaaaacat tacgagggcg aatatatacg cgacatcgct
    12901 gccgacataa aatcacaaca cggcgaatcc tggaaatctt atgacttcga acctttcaag
    12961 tcctttgccg aggatatctt atttgccaat atccgaaaag tcttaggtcg tatcggacta
    13021 aaatttgacg tttattacaa tgaagactcc ttgtacaaag aaggtaaaat tgaagctttg
    13081 ctcaaatcgt ttgcggctaa aaacctaacc tatgtcaaag aaaacgctac ctggctaaaa
    13141 acatccgaat tttcttatga tcacgggcct aaacccgaaa atgataaagt catcgtaaaa
    13201 tcaaccggag agccaaccta tcgtttgccg gatatggcct accatgttac caaatttgaa
    13261 cgcgggtttg atcatatcat tgacattttt ggcgcggatc atatcgccga atatcccgat
    13321 gtattagccg gactcaaggc cttaggttat gacacctcgc atgtgcgcgt tctcattcat
    13381 cagtttgtga cattgatgaa agagggggaa attctcaaaa tgtccaaacg taaagccaat
    13441 ttcgtaacta ttgatgaatt gattgatttg cttgggccgg atgtgtttcg ttattttctc
    13501 atcatgcgca gccacaattc gcatcttaat tttgatctcg aattggctca aaagcagtct
    13561 atggaaaatc ccgcgtacta tatacaaaat gcccacgcgc gtatttgttc gattgagaaa
    13621 aaagccggtg aacgcgatat tttgttctct gtttccaatg aaattaaatt ggatttattg
    13681 aaagcagacg aggaaaccac catcattgaa aaacttctac aatttccgga actcactgcg
    13741 cggcttgcta aaaatctcga accgcatccg ctcactacgt atcttgagga attggccggc
    13801 gtatatcatc attatcagac agcaggtaag aaaaatccag atctgcgtgt tatcacggag
    13861 gataaagctt tgtctgaagc acgacttgca ttgtgtatcg ctgttcgcat cgttttacgt
    13921 cgtggtcttg atattttagg tatccatgct ccggaatata tggcacgcga tcaggccgat
    13981 gaaactgttt gaaataaatt gactttccgt gaggcgttgt atacattgcg ccgcatttaa
    14041 ttgaaaattt tcaggagaaa aatgtggata tattggtcgt cggttcggtc gctttagatt
    14101 cggtagaaac accttttggc aaagtcgaag acgccgtcgg aggttctgct acttttttca
    14161 gtgcagcggc cagctatttt gctccggtca atcttgtggc ggtcgtaggg aacgactacc
    14221 ctatgcatgc catcgaatat cttaaatcac gccgcgttga ttttaaaggg ctgcaaatcg
    14281 aacaaggcga gacattccga tgggcaggtc gttataatta tgatctgaat caacgcgata
    14341 cgatttacac ccacctgaat gtatttcaaa acttcaaacc acatattccc gatgcgtata
    14401 aatcatcaca atatgtgttt ttaggcaata tcgctcctga gctacagttt gatgtattgg
    14461 atcaagttca atcgcctaaa ttggtcgctt tagatacaat gaatttttgg attgagggta
    14521 gtcaaaaagc gttacgcaaa gttctggaac gcattgacat ctttattctc aacgactccg
    14581 aagcaagaca actggccaat caacccaatt tgatcaaagc cgccaaagaa atttttaaaa
    14641 tgggtcccaa agcgattgtc attaaaaaag gtgaatatgg cgcggcgctc ataacacctg
    14701 atgctcaatt taatgtgccg gcttatcctt tggaagacgt gtatgatccg accggcgccg
    14761 gtgacacatt cgccgggggt ttcatgggtt atattgctaa aacggatgac gcatcatttg
    14821 aaaatctcaa gcgtgctgtg gtctatggca gcgcgatggc ctcattttgc gtacaagact
    14881 ttagtatcaa cgccttaaaa gagctttccc aagataaaat ttttgatcgc gttcttgcat
    14941 tcaaacaact cagccatttt gaagaaaaat agagtggata tttttgtatg aaactgagcg
    15001 aaattcttaa cgccaaactc attcgtatcc cgctactcca atctaacaag caggatatca
    15061 ttttagaaat gctggatgtt ttggttgaat ccggaaaaat cagcgatcgg gaaaaagtat
    15121 tacaatccgt gcttgaacgc gaacaaatga tgagtaccgg tatcggtaac agcgttgcta
    15181 ttccgcacgg gaaatcctcc ggcgtggaag agctgcaagt ggctttggga atcacggctc
    15241 aggatatcaa ttttgacgcc ttagacggca aacctgtacg tatcatattt atgctcgtcg
    15301 gacccgaaaa ggcttccagt ctgcatatca aaatgttatc tcgcatttcg cgattgctca
    15361 atcaggcacc atttcggaaa aaactgaccg atgcccaatc ggccgatgaa ataatgcaaa
    15421 tcatttcaga tgaagaaaag cttttggaag gttaatccgg aagcgtaatg attggttttt
    15481 cgcggtggca tttatatagc aaaatattat tgccgataat ctgaacaatt tgtgactgtg
    15541 tcccttcgct gatcatttca gctacttctt ccttatccaa atcacagccg tcttgcactt
    15601 tgaccttgat caattctttg gtgttaaaac cgttattgac ggataggtac gtattatccg
    15661 taataccgga cttccctacc gaaatggttg ctttaatggc gtttcccaag ccacgtaaaa
    15721 aacgtttttg ttttcctgtt aattccgaca aatgatcctc aatcgttggg taattttctg
    15781 tgtttatacc aagctgcaca aaccagtata atgccggacg ctacaaatgt caacggccaa
    15841 tacgtacgga tgataaatac ccaatcgtat ggaaacatat aaaaattacg tgaaatatga
    15901 attcctccaa aaccgatcag agaaagtcct gcaaccaacg aaaaccagcg tcgcatattg
    15961 aagaaaaaat tgatgacaaa agcgataccg cttatcagaa aatacactgg ccatagcgta
    16021 cgcatggcga gtccttcctg cgtgtcaagg tacaagtgga ataccccgat caacaataac
    16081 gtcagaccaa aaaacacgta atattttttg gttacgatcg tcttgattat gctcacgccg
    16141 ccaatgataa tcaatccgta acgctccatc caaccccagc tcaatacact catattccat
    16201 agtatccaaa acgtaccgat cacaataagc cataaacctg taaacaaata cttcttattg
    16261 ctgtcggacg cgctatcggg cttcacattt gataccgcgg cgtcggatgc gttgtattcg
    16321 ctttccgttt gcataacgat ctcctataat ttcttttccg atgcggcgcc aaaaactttt
    16381 tgatctttgt catcaggggt cagaagtccc acgagcgcat atgcgatcaa gccgacggga
    16441 aatgcaataa tcgtgataag cacccatgct atacgcacaa tcgaaacatc aagattccaa
    16501 tgtttagcag cgccggcaca aacgccgaat atttttttat tttcagacgc acgacgaaac
    16561 gtactttcac ttgttttatt aaaaaatccg gccgaaccgg caaaccggtt acgcgatgct
    16621 atcaaaaaaa ttccggcaat aataaacacc gtcggtacta aaaatttcca tccaaaatgc
    16681 cgccagaaaa aatggaaatt aaaaagattc atcgtatcaa ggaacattat cgcaccaatc
    16741 aatatcaata tggcgcccca aaaaaaacca tggtttacag cgcttcgttt ttcgatcaat
    16801 tccatatcgg gatcctgcgt caaactgacg ggctcgcggg gtacaagaac catcgccgtg
    16861 atataagcca ccacactaat acaatccaca aacgcaaaaa caacaaacaa aagtctcact
    16921 aaagctacat tcacattaaa atattcggcc agacctgcac acacgccgtc caccatttta
    16981 tcacgacgcg acttaaaaag ttgctttgtt gtttccatgg tatctcctga ttcaaaataa
    17041 tgtccataga taagaacgtg tatcttccaa aaatgttgca ttttttagtt aatttgttta
    17101 gttccaatgc gaggagattt gcattgaata atatactact actgagcatt tgctttattt
    17161 tgggtatagg attgcgcaaa agcggacgtt tccatgaaaa aacggcgacc gtgcttaatg
    17221 cttttattat tcacatcgcc ctacccgcct taacattact gcatgtccat acgttaaaaa
    17281 taagtaccga tatatggctc cccgccagca tggcatggtt tttctttgca atttctgctg
    17341 taacttttta tgggctcggc catctacttc attgggatag aagcactacc ggctgtatga
    17401 tgcttgtcgg cggcctcgga aatacctctt ttcttggtgt accgatgatt gaaacctttt
    17461 tcggaaaaga aggcattccg atcgggcttg tcgtcgacca actcggatca tttcttgtaa
    17521 tatcaacctt ggggatctgg acagtcaatt attatcaagg ccaaagtgtc tctttgaaaa
    17581 tgttaatcaa acgcatcatc gtttttccac catttatagc gctaatgctt gctgtagcac
    17641 tcatacctta tgaatttccc gcacccttga cctatacgct agatcggctt gggcaaacat
    17701 tagcaccgct tgcgctgttc tctgtaggtt ttcaaattca tttttctaaa ataaattcgc
    17761 ttcgcatacc acttgtttta ggtttgagct tcaaattgct catcgcaccc gctatgcttt
    17821 ttattttgta tagcacgcta acggccgatc gctatttgct ttttgacatt acgatttttg
    17881 aagctgcgat gccgcctatg atcacggcgg ctattttggc gacagagcac gatctgcgac
    17941 ctgaactggc ctccctgatg gtaggtatag gtatcatcgt atccatgttt acgttaccgt
    18001 tttggtgggt acttcttact ctctaaatcc cgcttgtaaa aaaaaaactt tttattatat
    18061 tgcgaccaaa attaaggaga ttctatgccg gacacaatta tcgttttaga aaccacgcaa
    18121 ggtacaatcg aagtaaaatt atttcctgac attgctccca aagcttcaga aaattttgag
    18181 cagcttgtcg aaaagggtta ttatgacggt attatttttc atcgcatcat taaagatttt
    18241 atgatacaag gcggtgatcc tacaggtaca gggcgcggcg gccaatccgt gtggggaatg
    18301 ccttttgatg acgaattcag tttcaaggtt caatttgata aaccgggtat tcttgccatg
    18361 gccaatgccg gcccgcgcac caacggaagc caattcttta ttacgacagt tcccacgccg
    18421 tggctcaata accgtcattc gattttcggt gaagtgatta gcgggtatga tgtggttaag
    18481 aaattggaag gggttaaaac taatccgatg gataaaccat tacaagatca aaaaatcatc
    18541 aaagcctata agaaagcttg atcggcacat gatgccctat cgcatacttt tatattataa
    18601 atttactgaa atatcggatt acgaaaaatt tgcggccgag caccttacat tatgccaatc
    18661 gctcggcctt ttggggcgga tcatcgttgc tccggaaggt ataaacggta cggtttcagg
    18721 tacggtagaa caaaccgaca tttaccaaaa aacaatactt tcggatccgc gtttttcgga
    18781 tatggttatt aagtccgatc catccgataa acacgccttt cgccgccttt cagtcaaagc
    18841 acgtacagaa attgttacat taggcccgga caatgtcaat ccgaccgaaa aaacgggtca
    18901 ttatttggaa ccacctgatt tttatgccgc aatgaaagaa ccggatgtcg tgatcatcga
    18961 tgcacgcaat gactacgagt acgacatggg tcactttaga ggggcaatac gacctgatgt
    19021 aagtaacttc aaagaatttc ccgcgtgggt tcgccaaaac ctcagttctt atcgtgataa
    19081 aaaagttctt acgtattgta ctggcggcat tcgctgtgaa aaatttaccg gtttgctgat
    19141 caatgaagga tttaaagacg tctatcaact gcatggcggc attgtaatgt acggtaagca
    19201 tcctgatacg aagggcagag actttgaagg caaaatgtat gtgtttgatg aacgcatcgg
    19261 cgtcgaagtc aaccatacag aaacagcgtc catcattact cggtgtttat actgtgaaac
    19321 cgtgacagac cactttgtca actgtgcaca cttggactgc cacaaaagtt tttatatatg
    19381 tgccgcttgt gaccgcgcca accaacgcag ttgctccgaa gcctgcaaaa atgcagatca
    19441 tcatgaatac cgtgtactcg gcgacgcgct tttcgaagcc cccttaacac cgcgcggctt
    19501 atttaaatcc atattaccaa cctcctgatt ttgccgccca aattcgttgc attttaatcc
    19561 tatattctat aggttatacg taacgtatta cgtataaata gaggcaatta tggatacagt
    19621 aaaacacttg ggtgagcttg ccatcggaag cagacttcgc cgactcagtg accgactttt
    19681 agccaatgta aatcgtatct atcaggaaca cgggataaat tttgaaacgc gttggttttt
    19741 gatgttttac caattgcatc tcaagtcgcc catgtctatt acagagattg cggcagctac
    19801 cggttttacg catccggcta ttatccaact atcggatgaa ttaattaagg ttggattgat
    19861 cgcatccgtt accgatacca cagataagcg tcgccgtctg atttctttgt cggccaaagg
    19921 catcgaaatg ctgggtcatt tgaaaccggt atggcatcaa atcgaagaag cgcaccgcca
    19981 gatcagtcgc gaaatcggta tggatttact ttccacgctt gagcgctacg aagaaatact
    20041 tgatgaacgt gacatctatg agcgtgtcac ccacgcgaca aaagtttctt ctgcctctga
    20101 tattgtaatt catacgtatg caccgcgttt caaaaaatat ttcaaattgc tcaatatcga
    20161 atggttaaaa aaatttttta caattgagcc attggatcga aaaatactat cccatcccga
    20221 aaccatcata aaagacggcg gtgaaatatt ttttgccgag tggaatggaa agatcgttgg
    20281 cacttgcgct gtactaaaca ataaagccgg aatttatgaa ctagccaaaa tggctgtaac
    20341 cgaaaaagcg caaggtaaac acatcggatg gaaactcgga tcgcatgcga tcacgtgggc
    20401 acagaaaaac aaaggcaaaa caatggtttt agaaacaagt cgaaaattgt atgccgcgtt
    20461 agccttgtat cgcaaattag gttttgtatt ggttgctaat cccgagccat ccaaatatga
    20521 acgttccacg atcaaaatgg agttgcgatt gaggtaagat tatcctccga ttttagatcg
    20581 ttttgcgata tactccctca aggctacatc aaagggcgtt ttagtatcca gcaaaacgta
    20641 atcgactcgg ctttctctgc accggcggcg ataataatca caaaacgatt ctacttcttg
    20701 agtgtaggct ttgcgaatgt gccaaggctg cgttgccaat tgttctccgg tttccaagtc
    20761 atcaaaaacc acatcgcctt caaaatccag agtgcgctcc gcatggtcca gcacatgaaa
    20821 tacgatcact tcatggccgt catgacgaaa atgccgcagt gcgtgcacga catcctccgc
    20881 ttcatccaaa agatcgctta tcagaattac aagccctcgc cgtttgatgc gttcggctac
    20941 ggcatgaaga ttctccgcaa tgcgtgtagt ttcgtgatgt gatatattct gcaaagcctc
    21001 ttcgatcgtt ttgatataac ccagcgttgc acgggggggc aaatacttac gcaatacttt
    21061 atcaaaaatg gcaagcccgg cagcatcttt ttgatgaatc atcaaatacg ccagcgaagc
    21121 cgccagatat ccgccatact gaagtttatt cacctcgccc gttccaaacg tcatggatgc
    21181 acttgtatca agcacaatat ggcatttgag attcgtctcc tcctcaaacc gcttcacgta
    21241 ataccgcgaa gtgcggccgt acactttcca atctatcgaa cgtatttcgt caccgggcat
    21301 atattgccgg tgctctgcaa actcaacgct gaaaccgtga tatgggcttt tatgaagacc
    21361 tgtaataaat ccttcgacta cgaaacgcgc aatcatctct agattggcta gccgcgcaac
    21421 cacatcgggt tgtaaatatt ttttgatgtc tgtcatgtct taaaaaaata atcccgcacg
    21481 aggcgggatc atgatactca gtgtttatta aattagaaat ccgatccgat cgaaaaataa
    21541 tgtttggcat tacctccgct aaatcgcggg tttcgaatat tccaggccag atcgtagcgt
    21601 aagataaaaa atcctacgaa cgcacgaata ccgaaaccat aacctatgcg tgcatcctga
    21661 aatacagggt ttccttcgcc ttttaaagtt tgcccccagt caaatcggct tttgttatat
    21721 acaatattac catttcggtc tatgtagcga tagtttgctt ttttggtttc gcccgttaat
    21781 tgatctacta caaattcacg atcctctccg ccccaggccg cgccaaagtc aacaaaagtt
    21841 acgccgcgta tttgttgaag aaacatcggt attggaaagc caagttgcat gaagtggact
    21901 aaaggaaaac gaaactcaaa attggaaata aagtaacgcg taccttgcaa ttcataataa
    21961 ttactcccgc gtaacggaga aacatacgtt gcaagaaagt cctcgaacgt atcaacaaga
    22021 atatcgccat tattgaatcg cggaagaatc caattatcca atccacccac aaaaaagcgc
    22081 tgaggattgc gtccgaaagt agctccaaag ctagcgcgtg ttgcgatggc ataaaacttt
    22141 ttaaaccaaa aatactttct tccgtctacg gttgccgtta caaaccgcag gccgttttgt
    22201 ccatatcccg gacttgccgt aatagccact tgcgcacgtt gaccatcaaa cggaccaaaa
    22261 tatgtattaa gcgaattatc attgatgaga gataaggtta aaaccgtatt attagattgg
    22321 cgcgaacgcc ctaatccggt tgaattttta ggcggcagcg aatcattacg tgcattccat
    22381 gaagtttccc gcgcaagaac aagttgcgta gcagaaaaat caattcggtt aaatttattt
    22441 atcggacgcg atgcattaag gcctaaacca taaaaacgaa acctcacaaa actatcagga
    22501 tatccgtcct gaccattaaa aaagaccggg ttaccattga tatctattcg ttcatccaga
    22561 taatccgtcg caaaagcata cgcggaatga aaggctgtaa taccataatc cgtacgattg
    22621 gctaaataat aatacgatcc tgtaaaactg ctatttttga gatcaaaaaa cagattggta
    22681 cccagcaaaa gcctatgatc gcccagcatg tcgctaaatg caaattgcgt tacgccctga
    22741 aaccctgtta aagtattgaa gccggcagta ccatatacca tgtccggaga aaattttaca
    22801 cgataacgtc gaacacggta atcacctttt tcgtttttat aaacattttc cggaagctcc
    22861 acagcttctt ccggttcgtc ttcatcctta actgaggaat tattattttc atcttccaat
    22921 ccggcaaata cgaagtttcg gtaagacacc ttcgattctt gtgttttcaa agaatcagct
    22981 ttagggtccg tcaccgttga agattcgcct tcattcgatg agacgatatt ttgctgtttt
    23041 ttaataccaa tttcatccgc taaagtttct ggtaaatcat gatctttacg aaattttttc
    23101 atcgaaatcg taatcggtag ctctacaggt ttcatttcaa acgggttctt caacgtataa
    23161 acatcgaaac cgcttctatt gaatccggag aacgtcaaaa atttaccatc tttgctgaga
    23221 gagggttgga aaataccact taagttattg gtaattgcat aatttttttt ctctttgaga
    23281 tcagcaacgt aaatattgct tacaccattt cgatccgaag tgaaaagaag tttactatta
    23341 tccgggcccc actccggcca cgagtcattc atttcatccg tcgtaatgcg ctccatgccc
    23401 gaaccatccg cgttcatcac gtagatatcc gtttgacggt actcaaaatt gatcatttta
    23461 aaaccgtcgg gttgtttacc cagaatatca cgtcggtccg atacaaaagc gattttactg
    23521 ccatcgcgac tataaaccgc acgactatca ctaaacacat cgtctgtcag tttagataat
    23581 ttaccgctcg ccaattcaaa ctcaaaaaca tcgctttggc caaaacgcat tccggaaaag
    23641 acaatttttt ttccatccgg gctccacgat ccaccaaata cgccttctag atccggccaa
    23701 ctatattttg taatatcgcc tgtcatcaca tctacaacca gtaaggcatc attatcgctg
    23761 gctttcgctg caaagaccaa ttttttgctg tccggtgacc aactcatacc cggactgagc
    23821 caatgtaatt cttcaaggtt aggcgttttt tctccggaca cgagcttttt caacggcatg
    23881 ccgtcgtatg ctgaaattaa cataatgtcc gcgtaacccg atttatccgt aagatatgcg
    23941 attttatcac cattcggaga aatcgtaggc gtcatgttat agaaattttc gtcctttaag
    24001 tgattggtca acgctttacc gatttctttg actgaaacca tctgtgaaat atcaggataa
    24061 taaattttac gttgatacgt cgcccactta tcggaaaact cttcttcttt catgccgaac
    24121 gtcgattcaa aagtagcatt cggcgttcct gcaacacgca gtttggtcat aaattcagct
    24181 accttttctc gcccgtattt ttcctccata tatcgaaaca cggtcggtcc cgccgtataa
    24241 acagcataac cacccatttg attcaatggc ggtaagtaag cttccatcac tgcatcgcgt
    24301 acaaacatat ccgattgggt attaaatgca ccataacgcg acgagtactc tgcaagacct
    24361 tcggcgaccc acagcggcac ctgcacgcga atccggccgg aaataatacc ttgaatcgaa
    24421 cctccgtaca tataatcatt catcaccccg tgtaccaact cgtggtggat cacgtgacga
    24481 aactgttcgt aagagccttc aaacggaatg actacgcgat ttttaaacaa ttccgtaaca
    24541 ccgcctatgc cttcggacat atattcgtcc aagacgttgg tttgttgaaa ttggttatgc
    24601 gaattataaa caaccaaagc gatgcggttt tgaatatcat aattgcgaaa gcactcttta
    24661 atatgaacgt acgcgctttc ggcaacttcg gccgtaaaac gcgcaatgtc ctcgccgcct
    24721 ttgtaataat aaatgtcaaa gtgtttagat tgaatataac gccagttgaa ctcctgatat
    24781 tgtaccttat tctggccaaa ccatttttgc tcttcctgag ctgcgacttt gccggtcaat
    24841 aaatagacgg tcaacatgat aaaaccaacg gggagcctga aaaaactccg catacgaaac
    24901 ctccttacaa aggaatattt taacctggaa atgactaaga gatatacgca gtaaatgatt
    24961 aaaggtttaa cggcacagct acaaaaaatg atttgccaga aacgagtcaa accgctatat
    25021 tccgcgcgct tattaaatag tctatacaaa aagacaacaa cattcaagca taaacacgac
    25081 ccgaggagta ccgcatgagc cgtcatactt tggctattct aaaacccgat tgtgtacgta
    25141 acaatcaaca aggcgccgtg atcgccgaaa tccagaaagc cggattcaaa atcatcgcga
    25201 tgaaacagac tcgtttgacg aaggcccaag ctgaaggctt ttacgaagtg catcgtcagc
    25261 gtcctttttt cggtgaactc accgagttta tgagctccgg cccgtgtgtg ccgatggtct
    25321 tagaaaaaga caatgccgta gcggcatttc gcacaactat aggcgctacg gatcccaaag
    25381 aagcggctga aggcacgatt cgtaaactat ttgcatccag taaagcggaa aatgttatcc
    25441 acggctccga ttcagatgaa aatgcaaaaa atgaaatcgc atactttttt gcccacagcg
    25501 aattgatcgc gatacgctag ttgtcataga acatttttac agcgcagggt tccggtataa
    25561 actgttaagc cttgcgctgt tttactttga ggacgcacta tgaatgcaga aatccttgtc
    25621 atcggcgatg aaattttact cggattgata acggacacca actccgcgta tatcagtcgt
    25681 cgtcttgctc aatacggtgt cgccgtcacg cgtattacca aagtcggcga tcaaacagaa
    25741 attatcgcgg atgcgctccg tgcatcatca agtcgagcct ctttggttat cagttgcggc
    25801 ggtttaggtc caacccacga cgataaaacg cgggatgcgg ctgctttatt cctcaatgtt
    25861 cccctgcaac tcaatgaaag cgcattaaaa gaaatagaaa ccctgtatgt caaagcaggg
    25921 cgtaccatga gtgctgcgaa ccgtgtgcaa gcgatgattc ccgagggtgc gatgtatctg
    25981 agtaatgaac gcggcaccgc gccgggtctt cactttatca aaggcgatac cgcgtttttt
    26041 tgttttcaag gcgtccccaa agaaatggaa tggatgaccg aaaactatct tattccattt
    26101 gtacaaaaaa aaccgtcatc gcatgtggtt cgct
//
LOCUS       DAOQGF010000039        25815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_882_length_25815_cov_6.198253, whole genome shotgun sequence.
ACCESSION   DAOQGF010000039 DAOQGF010000000
VERSION     DAOQGF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25815
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_25815_cov_6.198253"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK937_07310"
     CDS             complement(<1..445)
                     /locus_tag="PK937_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32414.1"
                     /translation="MDTAIIDWRTPAIKRRLIEDDPLDWPTIFGNHNPIDLEIGIGNG
                     SFLVPFCAHNKLRNMVGIEIDGFYLKKADRKLNQTGLTNGRLLIGDAKLFTWRLFGKE
                     TIADVYINYPDPWFKKRRLKRRVINTLMLQMLATRMTGVLTIATDD"
     gene            complement(453..1235)
                     /locus_tag="PK937_07315"
     CDS             complement(453..1235)
                     /locus_tag="PK937_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH32415.1"
                     /translation="MSTFQYLTLEIAEKIAIVGLNNPPGNSLKMPLLQELDRMMDELH
                     ANPEAKVIVITGAGKTFASGADIEEISKLTSQQQAKEMSAFGQKVFLKIENGPKPVIA
                     AINGFCLGGGLELAMSCHMRVASDRSFMGLPEITLGIIPGFGGTQRLPRIVGLSKASQ
                     MILSGDQVRSEEALAIGLVNMVVSKDTLLDDVKKLAAKMTNKSAVAIKQALKSMKQGA
                     SLEMAASMEVESSCFAELYNSADMKEGVSAFLEKRKPNFQDR"
     gene            complement(1296..1853)
                     /locus_tag="PK937_07320"
     CDS             complement(1296..1853)
                     /locus_tag="PK937_07320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH32416.1"
                     /translation="MRVRKFFLLLWIAQLTSLTAQSENTLQPGSTAPNFFLKYLDQSE
                     FYSKDYYGEPRSLPKAKKERNAVIISFFASWCGPCRKEIPELEKLQSKYADVRIFLIN
                     VAESQDKVLSFLAENPVSLPVLMDIYGKTAEKFDVKDKGLSLAVLPTLVMIRKDGTVH
                     FYKKGYTAGDEKKIEEQILTLLAQP"
     gene            complement(1967..2410)
                     /gene="rplI"
                     /locus_tag="PK937_07325"
     CDS             complement(1967..2410)
                     /gene="rplI"
                     /locus_tag="PK937_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNH32417.1"
                     /translation="MKVILKQNVDTLGQAGEIVDVKPGYARNFLVPKGFAMVATKANE
                     AAFENEKRMIAARLAREKALAQEMVQKLEAISVTIPVATGEEDKLFGSVTSQDIAEYL
                     AQQGHDIDKRKILLDEPIKALGLYNVDVKLFTDVIGKIKVWVVKK"
     gene            complement(2446..2712)
                     /gene="rpsR"
                     /locus_tag="PK937_07330"
     CDS             complement(2446..2712)
                     /gene="rpsR"
                     /locus_tag="PK937_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNH32418.1"
                     /translation="MAEKKFEKKRREPRGGMNKPKHCPFCEEKALYVDYRDTKRIMKF
                     TSEQGKILARRTSGVCATHQRQLVSAVKYARHLAMIPFVSDMAR"
     gene            complement(2746..3141)
                     /gene="rpsF"
                     /locus_tag="PK937_07335"
     CDS             complement(2746..3141)
                     /gene="rpsF"
                     /locus_tag="PK937_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNH32419.1"
                     /translation="MSKKTRKGSAGQSRFYETVVVFDAQTGDDVIKGIVDKIKEVLTS
                     HSAVNVAVDEWGKRKLAYVINKKTYGHYVCVEFEAPGSALQALNDYYHITEQIMRHMT
                     MLVDSRLRAERKREKTAMPKEAVADEAAI"
     gene            complement(3282..5366)
                     /locus_tag="PK937_07340"
     CDS             complement(3282..5366)
                     /locus_tag="PK937_07340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNH32420.1"
                     /translation="MLLYIALASGFLALAFAAYKAMWISKQEAGNDRMKEIATAIADG
                     AMAFLMREYKVLVIFVIVAGALLAVSGSMEEHSSWLVGISFAVGAFCSALAGFFGMKV
                     ATKANVRTTNAARTGLQNALQIAFSGGSVMGMCVVGLGVIGLTVLYFVYSNMFTDTAK
                     VLNVISGYSLGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDVAGMGADLFESYVGSIVGTMILGAAMIVGTNAGMVPVLLPILLAGVGIIAS
                     IVGTFFVRTKEGGDPQVALNTGTFVAAGAMIVASYFAIHKLWPSVDLYDTMDTAKLVT
                     ANDVFLATVFGLIVGVLVGIVTELYTGGGRSHVNRIVEQSVTGPATNIISGLAVGMES
                     TAIPVVLIAVGIIGSYYFAGLYGIAVAALGMLSTLGIQLAVDAYGPIADNSGGIAEMS
                     HLPEEVRGRTDKLDSVGNTTAAIGKGFAIASAALTALALFTAYMSQVGLKTLDVANPK
                     IMAGLFVGGMLPFLFSSMAMSAVGKAAMDMVKEVRRQFKEIPGLLEGKPEAKPDYKRC
                     VEISTQAAIREMVVPGLMAIAVPVAIGFIPGLGKEALGGLLAGCTVTGVCMAIFMANS
                     GGAWDNAKKSFEGGYTLKDGTVVKKGSDAHKAAVVGDTVGDPFKDTAGPSLNILIKLM
                     SVVALVIAPLLK"
     gene            complement(5442..6017)
                     /gene="pth"
                     /locus_tag="PK937_07345"
     CDS             complement(5442..6017)
                     /gene="pth"
                     /locus_tag="PK937_07345"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNH32421.1"
                     /translation="MKLIVGLGNPGSRYESTRHNVGFMLIDKLARKHSVPMREGKGEY
                     FIGECMIGDERTFLMKPTTYMNNSGLAVRPFMAFFKIPVSDVLIACDDVVLPLGTIRM
                     RKEGSDGGQNGLKSVIYQLATDQIARLRIGVDHPLRCKMSLSDFVLSRFLEDEMAVLN
                     LVMDRATLAAESFVQHGVTRAMNQYNGGLPA"
     gene            complement(6095..6745)
                     /locus_tag="PK937_07350"
     CDS             complement(6095..6745)
                     /locus_tag="PK937_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNH32422.1"
                     /translation="MQETTLTAERRPAGKHSAKHARKQGKVPGVFYGYGQENVSIQLD
                     AMSLAKFLMTEHTLATITIDGKEHRVIVRDFDKDPVTGRLVHVDVMAVRMDRPIDVYV
                     PIVFIGSPIGVRTKGGIFQHDMTEFHIKCLPNDIPAHLEVKIDDLDLNHAVHIRDLNY
                     PNITILNPASESVCTVVIPKALESVLAEAQAAPTEPEVIAKGKEVAGEGEKAPEKK"
     gene            complement(6813..7742)
                     /locus_tag="PK937_07355"
     CDS             complement(6813..7742)
                     /locus_tag="PK937_07355"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNH32423.1"
                     /translation="MKYDIMLVSGRANRVFAEGIAKRLNIYLGKVEIVNFSDGEIFVK
                     YDENVRGKDLFIVQPTFPNSDNLMELLIMIDAAKRSSAARITAVIPYFGYARQDRKDQ
                     PRVPITAKLVANLLTTAGVDRILTMDLHSAQIQGFFDIPMDHLYSSAVLTEYWRGILK
                     KDAVIVSPDMGGVKLARAYAKRLELDFSIIDKRRPKANSVEIMNIIGHVEGRDVLLID
                     DMIDTAGTLTQAASALKEHGAQDIYAACTHPILSGNAIEKIMRSPIKKVVATDTIPMR
                     ASCEKIEILSVTHVFAEAISRIHKAESISVLFN"
     gene            complement(7813..7884)
                     /locus_tag="PK937_07360"
     tRNA            complement(7813..7884)
                     /locus_tag="PK937_07360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7850..7852),aa:Gln,seq:ttg)
     gene            complement(7912..8811)
                     /gene="ispE"
                     /locus_tag="PK937_07365"
     CDS             complement(7912..8811)
                     /gene="ispE"
                     /locus_tag="PK937_07365"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNH32424.1"
                     /translation="MPVNEVQLNAYAKINLGLRIHEKRGDGYHNIETVFKIISLHDRV
                     AIRLNDRGGARLTCGLAGVPTDESNIMMKAVRSLEKKSDRPIHTDIHLEKHIPMGAGL
                     GGGSSDAAAVLVALSRLLGLSLTGEELMHLGASLGSDVPFFVGFYLGHGATAKGQGRG
                     EVLSYFRWPLTEHVVVVYPNVHVSTPWAYQNFRTAIPSTVDTENERFNLTKRIESAIL
                     SAPLEKGLFFDNDFEYLVFREYPWIRTAQGVLVDLGAKVARMSGSGSTLYGLFDTVPE
                     FETLRERLGDAAIFRCRFIDSVK"
     gene            complement(8817..11090)
                     /locus_tag="PK937_07370"
     CDS             complement(8817..11090)
                     /locus_tag="PK937_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNH32425.1"
                     /translation="MQRKRREDDDMPESSDDHAKEIRIPVKPGHEPKKSALNKTNHSS
                     AWILALKLFSAVVFLLLTVVIFFAYYFRDDLPSIEQLEVYEPKLSTKVYSADGKVIKE
                     FFRERRTRIPIQEIPKSITEALLATEDHRFYDHWGMDIIRFVKSAMVNTLTLSSSEGF
                     SSLTQQLARNLYLSKEKTLSRKFKEVLTAIQIEKTYSKNEIMEMYLTHMYFGHDAYGI
                     HAAAKIFFHKDVRELSLAESAYLVGHLQAPSFYMNNIQAANTRKNTVLYRMREVGYIG
                     ETEFSAARQEKIQTFPRRDEDVIGYAPYFAENVRRELEYLSDSLHFNVYEDGLNVFTT
                     LDTRAQAAAEKAYEENVKERFGPLDKFGAEYLAANAQQYLPKYMRREGVSPERIERLL
                     ASPRAVDSLSRFYGTVQSGFVAVDPSNGHVLALIGGRDFKKYKLNHVTQIARQPGSTF
                     KPFLYTAAIDNGYSPNFKLLNQDVVIITEDGKRWTPQNYDGGRGGPTTLRDALATSLN
                     LVAIRLMQEVVPPATVITYAQKMGIQTTLQPVDALALGVFDVVPMQIVSAYGVFANKG
                     IRVDPVFITRITDKDGNLLYQHIPKYKEVLSAETAYIMAEMMKSSIDSERGSGRLARR
                     FGFKRPAGVKTGTTQKWSDGWYIGYTPQIVAGVWVGYDTYELNLSARNPGEVIAAPMW
                     GRFMLDAHEAMGLPEADFEMPPGVVRLEICEDSGQLAITACPKKYKEVFNAKFQPTDP
                     CAVHKTTVGKKKKGRGY"
     gene            complement(11095..11700)
                     /locus_tag="PK937_07375"
     CDS             complement(11095..11700)
                     /locus_tag="PK937_07375"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNH32426.1"
                     /translation="MNRRVTVAITGASGVIYAVRLLRYFLTHAYEVDFIISDAGKINF
                     KVEMDWDLDKKGIVEFLKNKFGHDIDKGKLREWPVQAIAAPMASGSMRRLGMVVVPCS
                     MKTLSGIAHGASTNLIERAADVALKENFPLIIVPRETPMNSIQIENMLTLSKAGARIV
                     PAMPAFYQKPQSIDDIADFIVARILHMLGIDDHGLFQPWGG"
     gene            complement(11697..12593)
                     /locus_tag="PK937_07380"
     CDS             complement(11697..12593)
                     /locus_tag="PK937_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNH32427.1"
                     /translation="MEYENKLDISFETKPQLPGIGTSFLILILGILTGFVFMGIFVVI
                     GKFIVPAESVFVLNKIGLVLGTMLFVLPALIYARIHKLDVVLLFRMRRVNLSVMIISV
                     VTALGLVVVTDTLDRWMAPAINAWLDSTIGVLSPELMSDRILEQLAEEMKIHSFTEGF
                     LLILGAVIAAGICEEILLRGLLQRSLEAHYPVLISVAISSLVFALIHFNPWGGIQIFI
                     IAIVLGAVAWRTQSILPTIVFHSVNNFTVLIFNNLEPSALLWYGDEHHVFPWVSMGGF
                     ICTLLGLWRIWILTSIEKEETK"
     gene            13063..13728
                     /locus_tag="PK937_07385"
     CDS             13063..13728
                     /locus_tag="PK937_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32428.1"
                     /translation="MKKILLTVALILKLGNISSLSAQDNQARIESKIDTLMSYQKMIY
                     SEVKKEDPLATRGAGFEINPARLLVMSDDRLSISGTYSMFAIDRRAEIAFPFLYEQGD
                     KRHGSTRLINLDAQYRRFLGDRQGGLYFSGGLRFTSLKGYEGSEWNWDEEISTNKVTE
                     NKLGVLCGVGYRYFTKSGFFWGTSLSVGRYFKYTDKSIQDNVLDDGKIIVDFEILKFG
                     FAF"
     gene            complement(13966..14118)
                     /locus_tag="PK937_07390"
     CDS             complement(13966..14118)
                     /locus_tag="PK937_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32429.1"
                     /translation="MKGMKLSHLLLCIFLIGEGLISILGLKFDGIHFVMGGIALAAGV
                     LPFLDK"
     gene            14211..15161
                     /locus_tag="PK937_07395"
     CDS             14211..15161
                     /locus_tag="PK937_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNH32430.1"
                     /translation="MNRTDRLIAIVLLLHSRKIIRAKDIAEHFDITLRTVYRDMKALN
                     EAGVPIAAEAGEGYSLVEGYHLPPVMFTHEEAAALLVGSELTRSLTDASLQTHITSAL
                     HKIQAVLPQDRKNYLDNLREALHIVTYPSRFKEGFRDDVLKTIQDAIVHRRILTIEYI
                     TNRDDRQSKRNVEPLGLVYYSNFWHLIAYCRLRKDYRNFRTDRITAIQVKDESFASRD
                     DFSLTQYLSQSRPVENLIQVRVKFTYEVVSYVRDRNYFGIVSEEKTEGGVIFTFLTPE
                     LKYLSGWLLGYGEHAEILEPKALKQIMYDEAVKLTQMYKP"
     gene            15180..16604
                     /locus_tag="PK937_07400"
     CDS             15180..16604
                     /locus_tag="PK937_07400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNH32431.1"
                     /translation="MEYISVFDIFKIGVGPSSSHTMGPWRAAQRFLRELDDTNRLCDV
                     TRVQAHLYGSLAKTGKGHGTDMAILLGLSGEDPVTTEVESIPSRIAKIRSSHNLGLLG
                     QHPIPFDTDHDIVFHYDASLPFHANGLRFVAHFKDGALFEEIYYSVGGGFVVRENDTE
                     HDPSGMQTVPFPINNGDELLQRCREKRWPISKIVFENEKVFRREDAIHSALLRIWKVM
                     TECVFRGCHTEGDLPGGLKVQRRASAMNRRLLNNQDYPTQEDWISAVQSVPKSFQDML
                     NWVSCFALAVNEENASYGRVVTAPTNGAAGVIPAVLHYYRYLCPNVHDQSVIRFLLTA
                     GELGSIFKKGSTISAAMGGCQAEIGVSSAMAAGALTECMGGNPDQVLMAAEIAMEHHL
                     GLTCDPIGGLVQIPCIERNTMGAIKAITASQIALNSDPKMAKVSLDAVIKTMWQTAKD
                     MDNRYKETSEGGLATNVPVNLPEC"
     gene            complement(16709..17872)
                     /locus_tag="PK937_07405"
     CDS             complement(16709..17872)
                     /locus_tag="PK937_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32432.1"
                     /translation="MPLLIVALVLFVILDIAIRMIVRRMQDEKQRKLREEALAVSLRM
                     DYSREAKTLKRAEVPAPKARILCVDDEPVILDSFRKILVIDGYNVDTVETGQEALGLV
                     QAHHYDFVFTDLKMPAMDGVDVVKSVKEMRPDMDVIVITGYATVESAVECMKFGAMDY
                     IQKPFTEEELLNFTRKTLIKRQDRIQKQLMPKVHITHVHGAEQFMTKEFTIPGGMFIA
                     RNHCWVSMETEGDVKVGMDDFAKKLIGKIDGFEFPNRGMNIQVGQPLFSVKQGNRIIH
                     FKSPVSGKVTEINHALVKDPDRILVTPYDNNWICIIDPDRVDDDIRHLKIGKSAVQFY
                     QEDLQNYQNEVKTMVKGNGHGKPGDIYTGEMQQLEDKDWALVTGHFFERELVS"
     gene            complement(17921..18712)
                     /locus_tag="PK937_07410"
     CDS             complement(17921..18712)
                     /locus_tag="PK937_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32433.1"
                     /translation="MSSIKDILAVDDEPVVLTAISKTCTSERLKVDCAESAAEALKRM
                     ESQDYRLIISDIKMPEMDGFQLLAEVKKRGITSGVIISTGYATVENAVHALSEGAIDF
                     IPKPFTADELLSALYRGLRYGTIVSSENKSGNILPYVPCPSRYYRLGYGSWVYPERDG
                     TAKIGVTDLFVKAMENLTAIELTPMLSDIYQGNSCAQMRDARGLMHAVMCPLSGQVIQ
                     VNEELTEAPSQIEKDPFFEGWFYRIIPTDLEYELPKLVSCSSDRL"
     gene            complement(18709..20574)
                     /locus_tag="PK937_07415"
     CDS             complement(18709..20574)
                     /locus_tag="PK937_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32434.1"
                     /translation="MPAWLDKIGFRLILAVGVTAILIIGVFSYFNIRSHTEVLLAEVE
                     RRANQLSETVKYSTRHDMLFNQRERIHRQIRDIGEQPGINRIRLLNKTGEIVYSSKPD
                     DIGKMVDKNAESCFVCHSDQKPIERLTISERTRIFRLHPDSVRIMGIINPIYNEASCW
                     EASCHVHEPEQKVLGVLDVTIPLADVDAQVQSSRLEMTIFALVSFVALSCIIGVFVYR
                     WVEIPVHTMVTATHEIAGGNLNHALPENRSDELGQLARAFNHMTRKMAEARLQLFQSD
                     KMASLGRLAAGVAHEINNPLTGVLTYSSYLMKRTASQPDIQEDLKVIVRETLRSREIV
                     KSLLDFARQSVPKKSAADICEIIRRSLSVTENQLRLHRVKVETNFNEDMPHVIVDSNQ
                     MQQVFINLLVNAADAMSESGGTIHIHLARINLSPYGTTQIRKALCNKNHNLMSPDIKI
                     NGLPTIRLKAKSEGAEGFLYLDPIYGSHQHEYGILSGNILSLQCPQCLTSVINESVRC
                     PKCNALTYSFEVAGKGVFRGCTRNGCDWQQWPIMDEAGVREFVEIKVRDNGIGIPPED
                     MSRIFEPFFTTKGQKGTGLGLSVIWGIIDNHDGKITVESIPGQGTTFTIRIPVKS"
     gene            complement(20577..21716)
                     /locus_tag="PK937_07420"
     CDS             complement(20577..21716)
                     /locus_tag="PK937_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNH32435.1"
                     /translation="MKMNSFLILMFWTVMVFGQKNNPLHNKTNLPCKTCHTCEFPTIK
                     DPCLIDCPRENLITIRASVYSAPEFIQLNRLSDRYAGVRFSHKRHAEMYDMSGGCGGC
                     HHYNTSGPVQSCAACHALKNENKPDKPESTPGLQAAYHRQCITCHKTWSAEAGCNSCH
                     DLKKSVVSQSEKNKKDSLLAISHRTRKEPEKIVYETDTDKGRIATFYHKDHTQLFGVS
                     CGQCHSQLSCVKCHDQKRSMNEPLVQNVINKTEEEKHAACFNCHKDDNCTKCHSNKEL
                     QPFDHGTRTGWPLANYHTRLICSNCHGANKNFKGLSRECNTCHTAWTMTTFRHAVTGL
                     VLSEDHLELSCKDCHQKNKYTTKPVCMPCHDDKTYPNDLPGRKLW"
     gene            complement(21713..22957)
                     /gene="hybB"
                     /locus_tag="PK937_07425"
     CDS             complement(21713..22957)
                     /gene="hybB"
                     /locus_tag="PK937_07425"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HNH32436.1"
                     /translation="MNAFEKPVPVEKKFFTAGVMILILLALNGIVFLAGRFLFGLGAV
                     TNLDNQYPWGLWIGVDVAAGVALAAGGFTSAALGHIMHREEYHAVIRPALLTAMLGYT
                     FVAMGVCIDIGRYYYIWHPLIMWNGTSALFEVGMCVMIYMSVLYIEFLPIVTERFIGR
                     VALTGILKALNKPLDRFLRLLDRGLSKSMFVFIIMGVVLSTLHQSSLGTLMVIAGAKM
                     HPLWQTPILPLLFLLSAVSVGFPMVIFESLLAARSFGLKPEMAVLSRLARIVGPLLAI
                     YLAFKIGDMFIRETFVYLNEPTLESMMFVIELVVGVIIPLRMFLSPGVRRSPLWLFVA
                     SGLVILGVLINRINNFIVAYTPPYATERYVPSFGEISVTVGFVALLVLVYRVCVMVFP
                     VISKPDPNGGPARKYAIKGLPL"
     gene            complement(22967..23851)
                     /locus_tag="PK937_07430"
     CDS             complement(22967..23851)
                     /locus_tag="PK937_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH32437.1"
                     /translation="MINHSRRRFIAAAALLGAAASGSGKPVQAASRNELSPDRRGVLV
                     DTTVCIGCRTCEWACRKAHGYDDEKLENYQSTDVFKKVRRPDETRLTVINQYSQQQNE
                     TFVKFQCMHCDEPACVSACIVGAFHKEEKGSVLWDTDKCIGCRYCMVACPFQVPAFEY
                     EKAFRPDIIKCDFCKQRTDKGERPACVAECPVEAMIFGTRAELLQIAKDRIRRHPEKY
                     KDHIFGETEVGGTAWLYLAERDFAEIGFPKLGRNPAPGVSESIQHGIFAYFVPPVSLY
                     ALLGGIMWLMKHREEPDD"
     gene            complement(24235..>25815)
                     /locus_tag="PK937_07435"
     CDS             complement(24235..>25815)
                     /locus_tag="PK937_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32438.1"
                     /translation="KNSDHSQTYRFVYPDVVKGTIVEEGYEIEYVNLLSNPPLQHNVA
                     LQYSMPVEKLTFRYAYPDWWEIRQKNISDTRKLKYTTQMDAKNRKRVLMYEARDIAAI
                     HDEPFSPFFKEMADYSAFMIAKLNMGNINYKGASSWSEVANIYKNYAIDRTSFFSKLL
                     ANNVGRTTDKVVASCKTPEQKLDSIITYLQTQMDVGHGASDFDELLIKKKGNAYLITG
                     LAQAMLLKAGIDAKYLLIHSAQDGYFDKQYFSASEMYTPAVLAVINDKAYVVFPYIEF
                     LPITYIPELYQGQEAIKITNQGYDGFYKVPDEVGSDNITEETYQLNIAEDGAVEVEEL
                     KTLHGSEAYGVRRDMARMNKEERDQSLKKMTTYSDGHTTITSSSIENLSDHRKPLIIR
                     LRYTIDNLVTITPEEVIFQTGGLFSPTSRIKFKSDSKERKNPIRIYNDEAFIKKITIQ
                     YPQTWELASTMQDVQFQNNFGSTSGKYAISKGAFKAEQTLRLIKTIQPKETVDDLWSV
                     AGKSSRLQLPNLIFKVNN"
BASE COUNT         7127 a         6250 c         5407 g         7031 t
ORIGIN      
        1 catcatccgt cgctatcgtc agcactccgg tcatgcgcgt cgccaacatc tgaagcatca
       61 acgtattaat cacgcggcgc ttgaggcgtc gttttttgaa ccaaggatcc gggtaattga
      121 tatacacatc agctatggtc tctttgccaa ataagcgcca tgtgaacagc ttagcatctc
      181 cgatcaatag tcggccattg gtcaaaccgg tttgatttag ttttcgatcg gcttttttga
      241 ggtaaaaacc gtctatttca attccgacca tattgcgcaa tttgttatgt gcacaaaaag
      301 gaaccaggaa gcttccgtta ccaataccga tttctagatc aatcggatta tgattaccaa
      361 agatcgttgg ccaatcaagc ggatcatctt cgataagacg tcttttgatt gccggcgttc
      421 gccaatcaat tatcgctgta tccatagttc gatcagcgat cctgaaaatt gggcttgcgc
      481 ttttcgagaa atgcagatac cccttctttc atatcggctg agttatataa ctcagcaaaa
      541 caggatgact cgacttccat cgaagccgcc atttcaagac tggcgccctg tttcattgac
      601 ttcaaagctt gtttgattgc taccgcgctt ttattggtca tcttggcggc cagttttttg
      661 acatcatcaa gcagcgtatc cttagaaaca accatgttca ccaaaccgat agccaatgct
      721 tcttccgaac gcacctgatc accggacaaa atcatttgtg aagctttgct taaaccgaca
      781 atgcggggca ggcgctgcgt accaccgaat ccgggtataa tacccagtgt gatttctgga
      841 agccccataa aagaacgatc cgaagcaaca cgcatgtgac aactcatggc taattccaat
      901 ccaccgccga ggcaaaaccc gttgatcgca gcgatcactg gtttgggacc gttttctatt
      961 tttaaaaaca ctttttgacc gaacgccgac atttcttttg cttgctgctg ggatgtcaat
     1021 tttgaaattt cttcgatatc cgcaccgctt gcaaacgttt ttcccgcacc ggtaataacg
     1081 atgaccttgg cctccggatt ggcatgcaat tcatccatca tacgatcaag ttcttgcaac
     1141 aaaggcatct taaggctgtt gcccggagga ttattcaatc ccacaatagc aattttctct
     1201 gcgatctcaa gcgtcagata ctgaaatgtt gacattggcg cttccttttt tggttcagaa
     1261 tatattattc agagcggaca attcaaactg taaaatcaag gttgggcaag tagagtcaga
     1321 atttgctctt ctatcttttt ttcatctcct gccgtgtacc cttttttgta aaaatgcacg
     1381 gtaccatcct tacggatcat taccagcgtt ggcagaacag ccagggacaa acctttatct
     1441 ttgacatcaa atttttcggc tgtcttaccg taaatatcca tcagaaccgg aagcgacaca
     1501 gggttttctg caagaaaact cagcacttta tcttgagact cagcaacgtt aatcaaaaat
     1561 atgcgtacat cggcgtattt ggactgtaac ttttcgagtt ccggtatttc tttacggcat
     1621 ggtccacacc aactcgcaaa gaaactaata atcactgcat ttcgttcttt ctttgcctta
     1681 ggtagcgatc tcggttcgcc ataataatct ttggaataaa attcgctttg atccagatat
     1741 ttcaaaaaaa agttcggcgc cgttgagccg ggttgcaaag tgttctcact ttgcgcggtc
     1801 aatgacgtca attgggctat ccataataac aaaaaaaatt tacgcacacg cattatcggc
     1861 tattctgtta aatccgaata atgtaagcgg ttcgaataac aaataaaaca gaaaaggtaa
     1921 cctgtagagc aggttacctt ttcacaaaca agtacctggc gttgcgttat ttttttacaa
     1981 cccaaacttt aatttttccg atcacatccg taaacagctt aacatccaca ttgtacaaac
     2041 cgagcgcttt gatcggttcg tccaacagaa tcttacgctt gtcaatatca tgcccttgct
     2101 gtgcaagata ttcggcgata tcctgagagg ttacagaacc gaagagtttg tcttcttcgc
     2161 ccgtagccac gggaatcgta acggaaatag cttcaagttt ttgaaccatt tcttgtgcca
     2221 acgctttttc gcgcgcgagg cgtgctgcga tcatgcgttt ttcattttca aaagctgctt
     2281 cgttggcttt ggtcgccacc atagcaaaac ctttgggcac aagaaaattg cgggcataac
     2341 ccggttttac atcgacgatt tcgccggctt gtccgagcgt atcgacgttt tgttttaata
     2401 tcactttcat aaaaaatctc cagagtttct tcgttgttac cgattttaac gagccatatc
     2461 actgacgaaa ggaatcatcg ccaggtgacg tgcatatttg actgcgctga ccaactgacg
     2521 ctggtgtgta gcgcatacgc cggaagtgcg gcgtgccaaa attttgcctt gttcagaagt
     2581 gaacttcata attcgtttgg tatcacggta atcaacatac agcgcttttt cttcgcagaa
     2641 cgggcaatgt ttgggtttgt tcattccgcc gcgcggttct ctgcgtttct tttcaaattt
     2701 tttctctgcc atggtcgtat cgttccttac gttttcgtta aatgattaaa tcgcagcttc
     2761 atcggcaacg gcctctttag gcatcgccgt tttttcacgt ttacgttcgg cacgtaaacg
     2821 gctgtctacc aacatggtca tgtgacgcat gatctgttcg gtgatgtgat aataatcatt
     2881 caacgcttgg agagccgatc ccggcgcttc aaactcgacg cacacatagt ggccgtaagt
     2941 ttttttgttg atcacatacg ccagtttgcg cttgccccat tcatctacgg caacattgac
     3001 ggcactgtga cttgtgagca cttctttaat tttgtcaacg atgcctttga tcacatcgtc
     3061 accggtctgc gcgtcaaata caacaacggt ttcataaaac cgtgattgtc ctgccgagcc
     3121 tttgcgggtt tttttggaca tggagtctcc tttggactat gttcggcgtc agcgtttgtt
     3181 aacacaactt acgttagcaa ttctgacgca ggattatgat gattctaatt tattaaaagg
     3241 gattcaatac atacgaagta caaatccctt tgataattca cttacttaag caacggtgca
     3301 atcaccagcg ctacaaccga catcagtttg atgaggatgt taagcgaagg acctgccgta
     3361 tctttgaacg gatcacctac ggtatcaccc acgacggccg ccttgtgcgc atcggagcct
     3421 tttttaacaa ccgtaccgtc tttgagcgtg tagccgcctt caaacgattt tttagcattg
     3481 tcccaagcgc cgccggagtt agccataaat atagccatac atacgccggt cacggtacaa
     3541 cctgcgagca aaccgcccaa agcttcttta cctaaaccgg gtataaaacc gatcgcaacc
     3601 ggcacagcaa tggccataag accgggcacg accatctcgc ggatagcggc ttgagtggaa
     3661 atttccacac aacgtttgta atccggtttg gcttcgggtt taccttcgag aagaccggga
     3721 atttctttga attgacgacg cacttctttg accatgtcca ttgcagcttt accgaccgcg
     3781 ctcatcgcca tggatgagaa aaggaacgga agcataccgc cgacaaataa accggccata
     3841 attttcggat tggccacatc cagtgttttc aaaccaactt gactcatata ggccgtaaat
     3901 aaagccaacg ccgtcaaagc cgccgacgca atggcaaaac ctttaccaat cgcagctgtt
     3961 gtattaccga cagaatctaa tttatctgta cgaccacgaa cctcttccgg aagatgcgac
     4021 atttcagcga taccgcccga gttgtcagcg ataggaccgt acgcatctac agcgagctgg
     4081 atacctaatg tagaaagcat accaagagcg gcgacagcta taccatacaa accggcaaaa
     4141 taatatgaac caataatacc aaccgcaatc aagacaacgg gaatggccgt agattccata
     4201 ccgacagcaa gtcccgagat aatgtttgtc gctggaccgg tcactgattg ttcgacgata
     4261 cgattgacat gcgaacgtcc gccgcccgtg tacagttcgg ttacaatacc gaccaaaaca
     4321 ccgacgatca aaccgaatac cgtagccaag aatacatcat tagccgtaac taacttggcc
     4381 gtatccatcg tatcgtataa atctaccgat ggccaaagtt tatggatggc aaaatacgaa
     4441 gcgacgatca tagcgccggc tgcaacaaaa gtacccgtat tcaacgctac ttgcggatca
     4501 ccaccttctt tggtgcgaac aaaaaatgta ccgacgatgg aggcaataat acctacgccc
     4561 gctaaaagaa tcggtaacaa taccggcacc ataccggcat tggtgccaac gatcatcgcc
     4621 gcacccaaaa tcatcgtacc gacgatagag cccacatacg actcaaaaag atcagcgccc
     4681 ataccggcca cgtcaccgac attatcaccg acattgtcag cgataacggc aggattacgc
     4741 ggatcgtctt cgggaatacc cgcttctact ttacctacaa ggtcagcgcc tacgtctgca
     4801 gctttggtat aaataccgcc gccgacacgc gcaaaaagag caatcgaaga cgcaccgagt
     4861 gaataacctg aaataacatt caaaacctta gccgtatccg taaacatgtt tgaatatacg
     4921 aagtacaata ccgtcagacc gataacaccg aggccgacga cgcacatacc catcaccgat
     4981 ccaccgctga atgcgatttg aagcgcattc tgaagaccgg tacgcgccgc attggtcgta
     5041 cgaacattag ctttggtagc caccttcata ccgaaaaatc cggctaatgc ggaacaaaat
     5101 gcaccgacag cgaaggaaat accgacaagc cagctagagt gctcttccat ggatccggaa
     5161 accgccagta aggcgccggc aacaatcacg aagatcacaa gcactttgta ttcgcgcatc
     5221 aaaaaagcca tcgcaccatc ggcaatagcg gtggcaattt ctttcatgcg gtcattgccc
     5281 gcttcttgtt tggatatcca catcgctttg taagcagcaa aggccaaagc caaaaagccg
     5341 ctcgccaatg cgatgtagag caacatttga tcgttcacag ggtttttctc ctcagataat
     5401 ttttattaaa acgaaaatcc gtattaacag cctaaatttt actacgccgg taaaccgccg
     5461 ttgtattgat tcattgcccg cgttacgcca tgttggacga aactctcagc cgctaaggtt
     5521 gcgcgatcca tcacaaggtt aagaaccgcc atttcatctt ccaaaaatcg tgataaaaca
     5581 aaatccgaaa gactcatttt acagcgtaaa ggatgatcta cgccgatccg tagtcgggca
     5641 atctggtcgg ttgccaattg gtaaataacc gattttaaac cgttttgacc gccatcacta
     5701 ccttctttac gcatgcgtat cgttcccaaa ggcaaaacaa catcgtcaca ggcgatcagc
     5761 acatccgata caggtatttt gaagaatgcc ataaaaggac gtacagccaa accgctgttg
     5821 ttcatatacg tcgtcggctt catcagaaac gtacgctcgt caccgatcat acattcgccg
     5881 ataaaatact cgcctttgcc ttcgcgcatt gggactgagt gtttgcgagc aagcttgtca
     5941 atgagcataa acccgacatt gtgtcgggtg gattcgtaac gcgaaccggg atttcctaat
     6001 cctactatta gcttcataat gtttcggcca cacatacggc aaatttgtta atttgccgta
     6061 gtatgcccgc aatgcagatt tcagaaattc cgaattactt tttctcgggt gctttttcac
     6121 cctcgccggc aacttcttta cctttagcga taacttcagg ttccgtcgga gcggcttgtg
     6181 cttctgcaag aacggattca agagctttcg gaataacaac cgtacaaacg gattcggaag
     6241 ccgggttgag gatagtaata ttgggataat tcagatcgcg aatgtgaacc gcatggttga
     6301 ggtccaaatc gtcaattttc acttcaaggt gagccgggat atcattgggc agacatttga
     6361 tgtgaaattc cgtcatatcg tgctggaaaa taccgccctt ggtacgaaca ccgatgggcg
     6421 aaccgatgaa aacgatcggt acgtacacgt caatcggacg atccatacga accgccatca
     6481 cgtccacatg cactaaacga ccggttacgg gatctttgtc aaaatcacga acaatgacgc
     6541 ggtgttcctt gccatcgatc gtaatcgtgg cgagcgtgtg ttctgtcatc aaaaatttag
     6601 cgagcgacat cgcatcaagc tggatcgaga cattttcttg gccgtagcca taaaaaacgc
     6661 cgggtacttt tccctgcttg cgggcatgct tcgcactgtg ctttccggcc ggacgccgtt
     6721 ccgctgtcag agtggtttct tgcatggtat catcccctgt tttttaggtg attattagat
     6781 aatccgtaca accgttgtac gaactgaatt ttttaattaa acaaaacgct gatggattcc
     6841 gctttatgaa ttcgcgaaat cgcttcggca aacacgtgtg ttaccgagag aatttcaatt
     6901 ttttcgcagc ttgcccgcat cggaatggta tcggttgcaa cgaccttttt gatgggcgaa
     6961 cgcatgattt tttcgatggc gttaccggaa aggatcggat gcgtacaagc ggcatatata
     7021 tcttgcgcgc cgtgttcttt gagagccgaa gccgcttgag tcaatgtccc cgcggtgtca
     7081 atcatatcat cgataagcaa tacatcacgc ccttcgacgt ggccgatgat attcatgatt
     7141 tcaaccgaat tggctttcgg acgacgctta tctatgatgg aaaaatccaa ttccaatcgt
     7201 ttggcataag cgcgagccag tttgacgccg cccatatccg gtgaaacaat gacagcatct
     7261 tttttcaaga tcccgcgcca gtattccgtc aataccgccg aggaatacaa gtgatccatc
     7321 ggaatatcaa aaaaaccttg aatttgtgcg ctatgcaaat ccatcgtcaa aatacgatcc
     7381 acaccggccg ttgtaagcag attggcaacg agcttcgccg tgatcggcac ccgaggctga
     7441 tctttacgat cttggcgcgc gtaaccaaaa tatggaatga cagccgtgat acgagccgcc
     7501 gacgaacgtt tggcggcatc aatcatgatc agaagttcca tcaaattatc ggaattggga
     7561 aatgtaggct gaacaataaa aagatcttta ccgcgcacgt tttcatcata ctttacaaaa
     7621 atctcaccgt cactgaagtt gacgatttcg actttgccga ggtagatatt gagtcgtttg
     7681 gcgatacctt ctgcaaatac acgattggca cggccggaga ccagcatgat gtcgtacttc
     7741 atctcaacca taacacgcct tttctactgc ttaaaaatca aaaagcccag agttccgggc
     7801 ttatgtatca tgctggggtg caaggactcg aacctcggaa tggatggacc aaaaccatct
     7861 gccttaccac ttggctacac cccagttata aaagaacaaa atccagaatt tctactttac
     7921 cgaatcaata aaacggcacc gaaaaatcgc cgcatcacct aagcgttcgc gcaacgtttc
     7981 aaactccggc acagtatcaa aaagcccgta caacgttgag ccgcttccgg acatacgagc
     8041 cacttttgcg cctaaatcaa ccaaaacgcc ttgagcagtg cgtatccacg gatactcgcg
     8101 aaacactaaa tattcaaaat cattatcaaa aaacaggcct ttttccaaag gcgcggaaag
     8161 tatagccgat tcgatccttt ttgtcaagtt aaatctttca ttttcagtat ctaccgtaga
     8221 aggtatcgcg gttctaaaat tttggtacgc ccatggagtt gaaacatgta cgttcggata
     8281 aacaactacc acatgttccg tcaacggcca acgaaaatat gataatacct caccgcgccc
     8341 ttgtcctttt gcagttgcgc cgtgaccaag ataaaatcct acaaaaaacg gaacatccga
     8401 acccaaagac gcaccgagat gcattagttc ctcacctgtc agcgataacc ccaataagcg
     8461 tgagagcgcg actaaaacag ccgccgcatc agagctgcct ccgccaagac cggcgcccat
     8521 gggaatatgt ttttccaaat gaatatcggt atgaattggc cgatcggatt ttttttccag
     8581 gctacgtact gctttcatca tgatattgga ttcatccgta ggcacaccgg cgagcccgca
     8641 tgtcagacgt gcgccacctc tatcattcaa ccgaatcgcg acgcggtcgt gaaggcttat
     8701 gattttaaaa acggtttcta tattgtgata accatcgcct cgtttttcat gaatacgtaa
     8761 gccgagattt attttggcat aggcgttgag ttgaacttcg ttaaccggca ttggtcttaa
     8821 tacccccgcc cttttttctt tttgcctact gttgttttgt gtaccgcaca gggatctgta
     8881 ggttgaaact tggcattaaa aacttctttg tatttttttg gacacgcagt gatcgcaagt
     8941 tgaccggaat cctcgcatat ttccaaacga acgacccctg gcggcatttc gaaatccgct
     9001 tccggtaacc ccatggcttc gtgcgcatcc aacataaatc ttccccacat cggcgcggct
     9061 atcacttcgc cgggattacg cgcactgaga ttgagttcat acgtatcata gcccacccaa
     9121 acaccggcga caatttgcgg agtatagccg atgtaccatc cgtcggacca tttttgcgtt
     9181 gtaccggtct ttactcctgc cggacgctta aatccgaatc gccgtgctaa tcgtcccgac
     9241 ccgcgttcac tatcaatact ggacttcatc atttccgcca tgatgtaggc cgtctccgca
     9301 ctcaaaactt ctttatactt gggaatatgc tgatacaaaa gattaccgtc tttgtccgta
     9361 atgcgtgtaa taaaaaccgg atcaacacgg atacctttgt tggcaaaaac accgtacgca
     9421 gatacgatct gcatcggtac cacatcaaaa acgccgaggg ctagtgcatc gacaggttgc
     9481 agcgtcgtct gaatacccat tttttgtgca tacgtgatca ccgtggccgg cggaacaacc
     9541 tcttgcataa gccgtatggc gacaagattg agtgaagttg ctaaagcatc cctcagcgtg
     9601 gtcgggccac cccggcctcc gtcatagttt tgaggtgtcc atcgtttacc gtcttccgta
     9661 atgatcacca cgtcttgatt gagcaattta aagtttggcg aatacccgtt atctatggct
     9721 gccgtgtaca aaaaaggctt gaacgtagag ccgggttgac gcgcaatttg tgttacgtga
     9781 ttgagtttat attttttaaa atcacgcccg ccgatcaaag ccaacacatg cccattggaa
     9841 ggatcgacgg ctacaaaacc ggactgcacg gtgccgtaga agcgactgag agaatccacc
     9901 gctcggggac tggcgagcag tcgttcgatg cgctcgggcg aaacaccttc gcgacgcata
     9961 tatttaggta aatactgttg cgcattggcg gcaagatatt ccgcgccaaa tttatccaaa
    10021 ggaccaaacc gttcttttac attttcctca tatgcttttt cagccgcggc ctgcgcgcgc
    10081 gtatccaatg tcgtaaaaac attgagtccg tcttcgtata cattaaaatg caaactgtcg
    10141 gacaaatact ctaattcacg acgaacattt tctgcaaaat acggagcgta tccgatcacg
    10201 tcctcgtcac ggcgaggaaa ggtctgaatc ttttcctgac gcgccgccga aaattcagtc
    10261 tcaccgatat agccaacctc cctcatacga taaagcactg tgtttttgcg agtattggcc
    10321 gcttggatat tattcatata aaatgaaggg gcctgcaaat ggccgaccag gtacgcagat
    10381 tccgctaaag acagttcccg tacatctttg tgaaaaaaaa tcttggcggc agcatgtatc
    10441 ccgtacgcat catgaccgaa atacatgtgc gtgaggtaca tttccataat ctcgtttttg
    10501 gaatatgttt tttcgatctg tatggcggtc aatacttctt tgaatttccg ggagagcgtt
    10561 ttttctttgc tcagatagag atttcgcgcg agttgttgcg tgagtgaact aaatccttca
    10621 ctggaactca gcgtcaacgt attgaccatc gccgacttga cgaaacgaat aatatccata
    10681 ccccaatgat cgtaaaagcg atgatcttcc gtcgcgagca aagcttcggt gatggatttg
    10741 gggatttcct gaatcggaat gcgtgttcga cgttcccgaa aaaactcctt gatgactttc
    10801 ccgtccgctg aatatacttt tgtggaaagt ttgggttcgt acacttcgag ttgttcgata
    10861 gaaggcagat cgtcgcgaaa ataataagca aaaaagatca ctaccgtcaa aagcaaaaac
    10921 acgaccgcag aaaacaactt cagagccaat atccatgcgg aagagtggtt ggttttattc
    10981 agggccgatt tcttgggctc gtgacccggc ttgactggaa ttcggatttc cttggcgtga
    11041 tcatcagatg actccggcat atcatcgtct tctctgcgtt ttcgttgcat gcggttaacc
    11101 gccccatggt tgaaaaagcc catgatcgtc aatgccgagc atatgcaaaa ttcgagctac
    11161 tataaaatcg gcaatatcgt ctattgattg cggtttttga taaaaggcgg gcatggccgg
    11221 cacgatccgc gccccggctt tggacaacgt caacatgttt tctatttgga tactattcat
    11281 cggcgtttca cgcggcacga tgatcaacgg aaaattttct ttgagcgcca catcggcagc
    11341 gcgttcgata agattagtag atgcgccatg cgcaatgccc gacagcgttt tcatggagca
    11401 agggactacg accataccca gacgtcgcat tgaaccgctc gccatcggcg cggctatcgc
    11461 ctgtaccggc cattcgcgca gtttgccctt gtctatgtca tggccaaatt tgttttttaa
    11521 aaattcgaca atcccttttt tatctaaatc ccaatccatc tcaactttga aattaatttt
    11581 accggcatcc gatatgataa aatctacttc gtaagcatgc gttagaaaat atcgcaaaag
    11641 acgaacggca taaataacac cacttgcccc ggtgatagct acagttacgc gtcggttcac
    11701 tttgtttcct ctttttcgat ggatgttaat atccagatcc gccacaatcc caataacgta
    11761 cagatgaagc cgcccatact aacccatgga aatacatgat gttcatcgcc ataccataat
    11821 aaggctgacg gctcaagatt attaaaaatg agaacggtaa aattattgac actatgaaaa
    11881 acgatcgtcg gtaatatact ttgtgttcgc caggctacgg cgccaagcac gatcgcaatt
    11941 ataaaaatct gtatgccgcc ccagggatta aaatgaatca acgcgaatac aagactggaa
    12001 atggctaccg atatcaatac aggataatgc gcttcaagag atcgctgcaa aagtccacgt
    12061 aatagtattt cttcacagat tcccgcagcg attactgcgc ctaaaatcaa aagaaaccct
    12121 tccgtaaatg aatgaatttt catttcttcg gccaattgct ccaaaatacg atctgacatc
    12181 agctccgggc tgagcacgcc tattgttgaa tcgagccagg cattgatcgc cggagccatc
    12241 cagcgatcca aagtatccgt aaccacgaca agtcccaatg cagttacgac ggaaataatc
    12301 ataaccgata gatttactct ccgcatgcga aatagtaaaa cgacgtctag cttatggata
    12361 cgtgcataaa tcaatgccgg cagaacaaat aacatcgtgc caagaactaa tccaatttta
    12421 tttaaaacga aaaccgattc tgcagggacg ataaatttac ctataacgac gaaaatcccc
    12481 ataaatacga atcccgtcag tatgcccaaa attagaatta aaaaagaagt acctataccg
    12541 ggtaactgcg gttttgtttc aaacgatata tctagtttat tttcatattc catggagccc
    12601 aatatgaaaa aaaacttcac taagggcaaa ctgaaattta aatgaggaat gaattatata
    12661 ttgaaaatat ccgatggaag attgacaaga gaaaaacata aactcaactc aaccgatgat
    12721 taccgccgaa ctatgacggc aaccatcgtc actaataccg ctactaggcc taaaactatc
    12781 aggcgagtgg tatagcgtac atataatggt atcgtattca tattatcacc tccttctatt
    12841 ccaaatataa ctgtgtttta ttatgcgatt attagtgttt tgttagagct ttatgatctt
    12901 gttgcaataa aaaacttgca atcgttgtat tttttcacac tcataaacta aaaaaacacc
    12961 ttataaatgc acaacttaca cgttggcatt gcatttgctc aaattcatgc gaatatgaaa
    13021 ctaaatcaaa ttcatacttt aaaaccaaac ttggagggaa aaatgaaaaa gatactatta
    13081 accgttgcgc taattctcaa actaggtaat atctcatcac ttagtgctca ggacaatcaa
    13141 gctcgtattg aaagtaagat tgatacactc atgagctatc aaaaaatgat ttacagtgag
    13201 gtaaaaaaag aagatccttt agccacacgt ggcgctgggt ttgaaataaa cccggctcgc
    13261 cttctagtaa tgtccgacga tcgcctttca atcagtggaa cctattccat gtttgccata
    13321 gatcgtcgcg cagaaatcgc attcccattt ctttacgaac aaggcgacaa acgccacggc
    13381 tcgacacgac ttataaattt agatgcacaa tatcgtcgtt tcctcggtga tcgccaaggc
    13441 ggtctttatt ttagtggtgg cttgcgtttc acaagcctca agggttatga ggggagcgaa
    13501 tggaattggg acgaagaaat atctacaaat aaagtcactg aaaataaact tggcgtttta
    13561 tgtggcgtcg gttatcgcta ctttacgaaa agcggtttct tctggggtac atcgctcagc
    13621 gtcggtcgtt atttcaaata caccgacaaa tccattcaag ataatgtcct tgatgacggg
    13681 aaaatcatcg ttgactttga aattctaaaa ttcggttttg cattctaaac tatatcatgt
    13741 acgtaagatc aaaagtataa ttcaacggag acacgtcatg aataattccg ttcaaaaatt
    13801 agagttgtat ccccctttga tcataggata cataacattg aaaataatca tcctgacgct
    13861 ttatcttata agcttgtgaa atttccggtt atcgcgtgat acgacaaaaa aagcgtcctg
    13921 cctttcggtg ggacgctttt accattttat tttttattta aattttcact tatccaaaaa
    13981 cggtaacaca ccggcagcta aagctatgcc tcccatgaca aaatgaatac cgtcaaattt
    14041 caatccaaga atacttatca aaccttctcc gatcagaaaa atacacaaaa gcagatgact
    14101 aagcttcatg cctttcatgg tgctctccta atgattggtt aattctataa gaatggatga
    14161 ttgaatctaa tgtacgaacg cattagattg caactttaaa ttgaaaaaac atgaatcgta
    14221 ccgaccgact tatagccata gtgcttttac tgcacagccg taaaattatt cgcgcaaaag
    14281 atattgccga acattttgat attacgctac gcacggtgta tcgtgatatg aaagcgctca
    14341 acgaagccgg tgtccctatc gcagccgaag ccggcgaagg ttatagcttg gttgagggtt
    14401 accaccttcc tcccgttatg tttactcacg aagaggccgc cgcgctttta gtgggttcag
    14461 aactcacacg ttctttgact gatgcttcgc ttcaaaccca tatcacatcc gcattgcata
    14521 aaatccaggc cgtcttacct caggatcgca aaaactatct tgataaccta cgcgaggctc
    14581 tgcatatcgt aacttatcct tcacgtttca aagaaggttt tcgggatgat gtattaaaaa
    14641 ccatacaaga cgctatcgtt caccgacgta tcttaacgat cgaatacatc accaatcgtg
    14701 atgaccgtca gtccaagcgt aatgtcgagc ctttagggtt ggtatattat tctaattttt
    14761 ggcatctgat cgcctattgc cgcttgcgca aagattatcg caatttccgc accgaccgga
    14821 ttacggcaat ccaagtcaaa gacgaatcgt ttgcttcacg cgatgatttt tctttaactc
    14881 agtacctttc gcaaagccgt cctgttgaaa acttgatcca ggttcgggtc aaatttacat
    14941 acgaagtagt gtcatacgtg cgggatcgaa attatttcgg tatcgtgtcg gaagaaaaaa
    15001 ccgaaggcgg tgttattttt acatttctca caccggagct aaaatattta tccggatggc
    15061 ttttgggtta tggtgaacac gccgaaatac tcgagccgaa agcactgaaa caaatcatgt
    15121 atgatgaagc ggtcaaatta actcaaatgt ataaacctta aaattaagta ctacactcca
    15181 tggaatacat cagcgtattt gatattttca aaatcggcgt aggtccttcc agttcgcata
    15241 cgatgggccc ttggcgtgca gcgcagcgtt ttttgcgtga attggatgat accaatcgac
    15301 tttgcgatgt gacccgtgtg caggctcatc tttatggttc tttggctaaa accggcaagg
    15361 gtcacggcac ggatatggct atattgcttg gccttagcgg cgaagatccc gttaccacag
    15421 aagtcgaatc catcccgtcg cgcattgcta aaatacgatc atcgcacaat ctaggtttat
    15481 tgggccagca tcctatacct tttgatacgg atcatgacat cgttttccat tacgacgctt
    15541 cacttccctt tcatgccaac gggttgcgat ttgtcgctca ttttaaggat ggagcattat
    15601 ttgaagaaat atactattcc gtcggcggcg gatttgtcgt acgcgaaaat gatactgaac
    15661 acgatccttc cggaatgcaa accgttcctt ttccgatcaa taacggcgac gaacttttgc
    15721 agaggtgccg ggaaaaacgt tggccgattt ctaaaattgt ttttgaaaat gaaaaggttt
    15781 ttcgccgcga agatgccata cacagcgcgc ttttgcgtat ttggaaagtc atgaccgaat
    15841 gtgttttccg gggttgccac accgaaggcg atcttccggg cggactcaaa gttcagcgcc
    15901 gagccagcgc gatgaatcgc cgattgctca ataatcagga ttatcccact caggaagact
    15961 ggatttctgc cgtacaatcg gttccgaaat cgtttcagga tatgctcaac tgggtcagtt
    16021 gttttgcact ggccgtgaat gaagaaaacg cgagttatgg ccgcgtcgtc acggctccca
    16081 cgaatggcgc ggcgggcgta ataccggctg ttttacatta ctaccgttat ctatgcccta
    16141 atgtccacga tcaaagtgtc atacgttttt tgctcaccgc aggcgaactc ggaagtatat
    16201 tcaaaaaagg ttccacgatt tctgccgcga tgggcggttg tcaggcggaa atcggtgttt
    16261 cttccgccat ggcggcgggc gccctcaccg aatgtatggg cggtaatcct gatcaggtat
    16321 tgatggcggc cgaaatcgcg atggaacacc accttggtct tacctgtgat ccgatcggag
    16381 gcttagtgca aataccatgc atcgaacgca atacgatggg cgcgatcaaa gccattacag
    16441 ctagtcaaat agccctcaat agcgatccga aaatggctaa agtttctctg gatgcagtta
    16501 taaagacgat gtggcaaact gctaaagata tggataaccg ttataaagaa acatccgaag
    16561 gcggattagc taccaacgtc cccgtcaatt tgccggaatg ctgatagttg gtattatata
    16621 tgtaaaaaaa cccctccaca tcctgcagag gggtttttca ttaacttaga aatcaaccta
    16681 ccgggtattg gccggcaaac ctgaaatatc aggaaaccaa ttcgcgctca aagaaatgtc
    16741 cggtaaccaa agcccaatcc ttatcttcga gttgctgcat ttccccggta tatatatcac
    16801 ccggtttgcc gtggccatta ccctttacca tcgtttttac ttcattctga taattctgta
    16861 aatcttcctg atagaactgc accgctgatt ttccgatttt gagatggcga atgtcatcgt
    16921 ccacgcgatc cggatcaata atgcaaatcc agttattgtc ataaggcgtt accaaaatac
    16981 ggtccggatc tttgaccagt gcatgattga tctctgtcac tttaccgctg acgggcgact
    17041 tgaaatgaat aatgcggttg ccctgcttaa cactaaatag cggttgacca acttgaatat
    17101 tcattccgcg atttggaaat tcgaatccgt caatttttcc tatcagtttt ttcgcaaaat
    17161 catccatacc aactttgaca tcgccttcgg tttccatact gacccagcaa tgattacgag
    17221 cgatgaacat accgccggga atcgtaaact ctttggtcat aaattgttcg gccccatgca
    17281 cgtgcgtgat atgcactttg ggcatcaatt gcttttgaat ccgatcttga cgtttgataa
    17341 gcgtttttct cgtaaaattg agtaactcct cttccgtgaa aggcttctgg atataatcca
    17401 ttgcaccaaa tttcatacat tcgaccgccg actctaccgt agcgtagcct gtaatgacaa
    17461 taacatccat atcagggcgc atttctttga cggatttgac tacgtccaca ccatccatag
    17521 ccggcatttt gagatcggtg aaaacaaaat cataatgatg ggcttgtacc agtccgagcg
    17581 cttcctgtcc cgtttcgacg gtatccacat tgtatccgtc aataacgagt attttgcgaa
    17641 aactgtctaa aataacgggt tcgtcatcca cgcagagtat ccgcgctttg ggtgcaggca
    17701 cttcggcccg cttgagggtt tttgcctctc ggctgtaatc cattctgaga ctcacggcaa
    17761 gtgcttcttc ccggagtttg cgctgctttt cgtcttgcat tcgacgtacg atcatccgta
    17821 tcgcaatatc gagtatgacg aaaagaacta aagcaacaat gagtaacggc atagaagcct
    17881 ccattcgtag gtgattgctt ttaatgaaaa tcgtatcctt ttataaacga tccgaactac
    17941 aggataccaa tttcggcagt tcgtattcca aatccgtagg aatgattcgg taaaaccatc
    18001 cctcgaaaaa cggatctttt tcaatttgac tgggtgcctc ggtcaattct tcattgacct
    18061 gaataacctg cccgctgagc gggcacatca cagcatgcat caatcccctc gcatcacgca
    18121 tctgtgcgca ggaattacct tgataaatat cggacagcat cggcgtcaat tcgatcgccg
    18181 taaggttttc cattgccttt acaaataaat cggttacacc gatttttgcc gtgccgtcgc
    18241 gctcaggata gacccaactg ccgtaaccaa gacgataata ccgtgaaggg cacgggacgt
    18301 aaggtaaaat attccccgat ttattttccg atgaaacgat cgtaccataa cgtaacccgc
    18361 gatacaaagc cgataatagt tcgtccgcag taaacggttt ggggataaaa tcaattgcac
    18421 cttcagacaa cgcatgtacg gcattctcga cggtggcgta tcctgtggaa ataattacac
    18481 ccgatgtaat accgcgtttt tttacttccg ccaaaagttg aaatccgtcc atttcgggca
    18541 ttttgatatc actgatgatc aggcgataat cctgtgactc catacgcttt aacgcctcgg
    18601 ccgccgattc ggcacagtcc actttcagtc gctcggatgt acatgttttg ctgatcgccg
    18661 tcaaaacgac gggttcatca tcgacagcca atatgtcttt gatactgctc atgattttac
    18721 cggtatacgt atcgtaaacg tcgttccctg accgggaata ctttcgaccg ttattttccc
    18781 gtcgtggtta tctataatgc cccaaatcac cgataggcca agcccggttc ctttttgccc
    18841 tttggtagta aaaaacggtt caaaaattcg actcatatct tcgggtggaa ttccgatacc
    18901 gttatcgcgc actttgattt cgacgaattc acggacaccg gcttcatcca tgatcggcca
    18961 ctgctgccaa tcgcatccgt ttcgcgtgca tcccctaaag acacctttac cggcgacctc
    19021 gaaactatat gtcaacgcat tgcatttagg gcaacgtacg gattcattga tgacggacgt
    19081 gaggcattgc gggcattgca aactcagtat atttcccgac aaaatgccat attcgtgttg
    19141 atggcttccg taaatcggat caaggtataa aaaaccttcc gccccttcgc ttttagcctt
    19201 gaggcgtatg gtcggtaacc cgttgatctt aatatcagga ctcatcaggt tgtgattttt
    19261 attgcacagt gctttgcgaa tttgtgttgt gccgtaaggc gaaagattga ttcgtgcgag
    19321 atgaatatgg atcgtacccc cggattcgct catcgcgtca gccgcattga ccaaaaggtt
    19381 gataaatacc tgctgcattt gattgctatc cacaatgaca tgcggcatat cctcattaaa
    19441 gtttgtttcg actttgacgc gatgcagacg caattgattt tcggtgacac tcaaggaccg
    19501 gcgaatgatc tcacatatat ccgcagcact ttttttggga acggactgcc gtgcaaaatc
    19561 taaaagactt ttgacgattt cgcggctgcg aagggtttca cggacgatga ctttgaggtc
    19621 ttcctgtata tccggttgtg aagccgtgcg cttcatcaaa taactgctgt atgtcaaaac
    19681 accggtgaga ggattattga tctcatgcgc gacaccggcg gccaatcgtc cgagcgaagc
    19741 cattttatcg gattggaaaa gctgtaaccg tgcttccgcc atttttcgtg tcatgtgatt
    19801 aaatgcccga gccaattggc ctaattcgtc gcttcggttt tcaggtaacg catgattgag
    19861 attcccgccg gcgatttcat gcgtcgccgt aaccatcgtg tgcaccggga tttctaccca
    19921 tcggtataca aaaacgccga tgatacaact gagcgcgaca aaggatacca aagcaaaaat
    19981 cgtcatctcc aatcggcttg actgaacttg agcgtccaca tcagctaaag gaatcgtcac
    20041 atccaaaaca ccgagcactt tctgttcggg ttcatgcaca tggcacgacg cttcccagca
    20101 cgacgcttcg ttgtatatcg gattgataat acccataatc cgtaccgaat ccggatgtaa
    20161 tcggaagata cgcgtacgtt cactgatcgt aagacgttcg atcggttttt gatccgaatg
    20221 acacacaaaa caactttccg catttttatc taccatctta cctatatcat ccggcttgga
    20281 tgaataaact atttcaccgg ttttattaag cagtcggatg cggttaatgc cgggttgttc
    20341 gccaatgtcc cgaatctggc gatgaatgcg ttcacgttga ttaaaaagca tatcatgccg
    20401 cgtgctgtac ttgaccgttt cactcagttg attggcgcgg cgttctactt cggcgagcag
    20461 tacttcggtg tgcgaacgaa tattgaaata tgaaaaaact ccgatgatga gtatcgccgt
    20521 cactcccacc gccaaaatga gtctgaatcc tattttatcc agccatgccg gcatatttac
    20581 cacaactttc tcccgggtaa atcattggga tacgttttat catcatgaca aggcatgcat
    20641 acaggttttg tggtatattt atttttttga tgacaatctt tgcacgacaa ttccaaatga
    20701 tcttcactca aaaccaatcc cgtgaccgca tgacgaaatg tcgtcattgt ccacgccgtg
    20761 tggcatgtgt tgcattcgcg cgataggcct ttaaagtttt tgtttgcccc gtgacaattc
    20821 gaacaaatta atcgtgtatg ataattggcc aagggccaac ccgtgcgtgt tccgtgatca
    20881 aacggttgca attctttatt gctatgacat ttggtacagt tgtcatcttt atgacaatta
    20941 aaacaagcgg catgtttttc ttcttcagtt ttatttatga cgttttgtac gagcggctcg
    21001 ttcatcgaac gtttctgatc atggcacttc acgcatgaga gctgtgaatg acactgcccg
    21061 caagacacac caaataactg agtatgatct ttgtgataaa atgtagctat gcgcccttta
    21121 tccgtatcgg tttcatacac gattttctca ggctctttgc gggtacgatg acttatggct
    21181 aggagtgaat cttttttatt tttttcactt tggcttacga ctgacttttt caaatcatgg
    21241 caggaattgc accctgcttc cgcactccac gttttatggc acgtaatgca ctggcgatga
    21301 taagcggctt gtaaacccgg ggtagattcc ggtttatcgg gtttgttttc attttttaaa
    21361 gcatggcagg ccgcacacga ctgtacaggg ccggatgtat tgtagtgatg gcaaccgcca
    21421 cacccgcccg acatgtcgta catttcagca tgccttttgt gagaaaaacg cacaccggcg
    21481 tatcggtcac tgagccgatt gagttgaata aattcaggcg ccgaatatac ggacgcacga
    21541 atagtgatca ggttttcacg aggacagtca atgaggcatg gatctttgat tgtcggaaat
    21601 tcacacgtat ggcatgtttt gcagggtaag ttcgttttgt tatgcaacgg attgtttttt
    21661 tgcccaaaca ccatcacagt ccaaaacatc aatatgagga aactattcat tttcataacg
    21721 gcaacccttt aatcgcatat ttacgggccg gaccaccatt gggatcgggt ttgctgataa
    21781 caggaaatac cataacacaa acacgataaa ccaatacaag taacgctaca aatccgacgg
    21841 taaccgatat ttctccaaac gatggcacat atcgctcggt cgcataaggc ggtgtataag
    21901 cgacgataaa attattgata cgattgatca gtacacccaa tattaccagg ccggatgcga
    21961 cgaaaagcca tagcggtgaa cggcgcacac ccggtgaaag aaacatgcga agcggaatga
    22021 ttacgcctac gaccaactca ataacaaaca tcatactttc aagggtcggc tcattgagat
    22081 acacaaatgt ttcgcggata aacatatccc cgattttaaa cgccagatag atcgctaaca
    22141 atgggccgac gatacgagca agtcttgaaa gcactgccat ttccggtttc aaaccaaaag
    22201 aacgtgccgc taacaatgat tcaaaaataa ccatcggaaa tcccacagat acggccgaca
    22261 ataaaaataa taaaggcagt atcggcgttt gccacagcgg atgcatctta gctccggcga
    22321 taaccatcag agttcctaat gaggactggt gaagcgtaga taacacgacg cccatgataa
    22381 taaatacaaa catggattta ctcagtccgc gatccagtaa tctcaaaaaa cgatccagtg
    22441 gtttattaag tgctttcaaa attccggtta gcgccacacg cccgataaaa cgttccgtca
    22501 caatgggtaa aaactcgata tacagcacgc tcatatagat catcacgcac atacccactt
    22561 caaaaagagc tgaggtgcca ttccacatga tcaaaggatg ccagatataa taatatcgtc
    22621 ctatatctat gcagactccc attgcgacaa acgtgtatcc taacatcgcc gtgagcaagg
    22681 ccggacgaat aactgcgtgg tattcctccc gatgcatgat gtgccctaaa gcggcgctgg
    22741 taaaccctcc ggcggctaat gcgactccgg ccgccacatc taccccgatc cacaaacccc
    22801 atggatattg attgtccaga ttggtcactg cacccaagcc aaaaagaaaa cgccctgcca
    22861 ggaatacgat tccattcagg gcgagcaata tcagtatcat aacaccggct gtaaaaaact
    22921 tcttttccac gggcacaggt ttttcgaatg cgttcatatg cttctcctaa tcatccggtt
    22981 cttcccgatg cttcatcaac cacataatgc cgcccaaaag ggcgtaaagc gataccggcg
    23041 gcacaaaata ggcaaagatg ccatgctgaa tggattccga aacgcccggc gccggattgc
    23101 gaccgagttt gggaaatccg atttcagcaa aatcgcgttc ggctaaatac aaccacgccg
    23161 tgcccccgac ttccgtttct ccaaaaatat gatctttata tttttcagga tgacgacgta
    23221 tgcgatcttt tgcgatttgt aataattcag cgcgtgtacc gaatatcata gcttctaccg
    23281 gacattccgc aacgcacgcg ggacgctctc ctttatcggt acgttgtttg caaaaatcac
    23341 atttgataat atcgggacga aacgcttttt catattcaaa cgccggcacc tgaaaggggc
    23401 acgctaccat acagtatcgg caaccgatac atttgtccgt atcccacaaa acgctaccct
    23461 tttcttcttt gtgaaacgcg ccgacaatac acgctgaaac gcatgccggt tcatcacagt
    23521 gcatgcactg aaatttcaca aaagtttcat tttgttgttg agaatactga ttaatgacag
    23581 tcaggcgggt ttcatcaggg cggcgaactt tcttaaatac gtccgtgctt tgatagttct
    23641 ccagtttttc atcgtcgtac ccgtgcgctt tgcggcaagc ccattcgcaa gtccggcaac
    23701 cgatacatac ggtagtatca accaaaacac cccggcgatc cggagaaagc tcattgcgcg
    23761 aagccgcttg tacgggttta ccgcttcctg aagcggctgc accgagcagc gctgccgcag
    23821 cgataaacct tcggcgactg tggtttatca taatttgcct cgcagcatga agaagtaact
    23881 attgcaattc caatcgtttc aaaaaacaat ttcaacaatg cacatacaac atacgtttga
    23941 aggcgtttta ctacaatgat accggtcagt gatgatcaaa ctttttcgct tgaaaaatag
    24001 ccgctttaaa tcatgtaagc acgtgacccg tgaggtcaaa tagagcaaaa gtattacaag
    24061 acactcataa aattttattt ctggcgccaa ttttgagatt tcatgataaa aggaaggaaa
    24121 aaataattca gattatggag tctgttgtta taaatttgat tatgtgaaat aataaagccg
    24181 gcaaaatttt atattcaccg gccatagaca cacatgggtt gttattagtt gttgctaatt
    24241 attaactttg aaaataaggt ttggtaattg aagtcttgac gatttgccgg caacactcca
    24301 aagatcatcg acggtttctt tgggttgtat ggtctttatt aaacgtaagg tctgctctgc
    24361 tttgaatgcg cccttgctaa tagcatattt ccctgaagtc gagccgaagt tattttgaaa
    24421 ctgaacatcc tgcatagtcg atgccaactc ccatgtttga ggatattgga tcgttatttt
    24481 ttttataaat gcttcatcat tataaattcg aatcgggttt tttctttctt tgctgtcact
    24541 tttaaatttg atgcgcgaag tcggtgaaaa aagtccgccg gtctgaaaaa tcacttcttc
    24601 cggagtgatc gtaacaagat tgtctatggt atatcgtaaa cgaatgatga gcggtttgcg
    24661 atgatccgac agattttcga tagaagacga agtgatcgta gtatgcccgt cactataggt
    24721 cgtcattttt ttcaaacttt gatcgcgctc ttccttgttc atacgcgcca tgtcacgacg
    24781 aactccatac gcttccgatc catgcaaagt tttcaattcc tctacttcga ccgccccgtc
    24841 ttccgcaata tttaattgat acgtttcttc cgttatgtta tccgagccga cctcgtccgg
    24901 taccttataa aagccatcat aaccctggtt tgtaattttt atagcttctt gtccttggta
    24961 taattcgggt atataggtaa tcggcaaaaa ctcgatatac ggaaaaacga cgtaggcttt
    25021 atcattgatc acggctagta cagcgggcgt atacatttca gatgcagaaa aatattgttt
    25081 atcaaaatat ccgtcctgag cgctgtgaat aagaagatat ttagcatcaa tcccggcttt
    25141 caaaagcatg gcttgcgcaa gccctgtgat caggtaagca ttgcctttct ttttgatcag
    25201 caattcatca aaatcactcg cgccatgccc aacgtccatt tgggtctgaa gatacgtgat
    25261 gatcgagtct aatttttgtt caggtgtttt acacgacgca acgactttat ccgttgtgcg
    25321 ccccacatta ttggccaata acttggagaa aaacgacgtt ctatcaatcg cataattttt
    25381 ataaatattg gccacctcgc tccaactgga tgcgccttta taattaatat tccccatatt
    25441 gagtttggca atcataaatg ccgaataatc ggccatctct ttaaaaaatg gcgaaaacgg
    25501 ttcgtcatga atagccgcga tatcacgcgc ctcatacatc aaaacccgtt ttcgattttt
    25561 ggcgtccatt tgtgtggtgt atttaagttt acgcgtatcg cttatatttt tttggcgaat
    25621 ctcccaccaa tccggatacg catagcgaaa agtaagcttt tcaacaggca tactgtactg
    25681 caatgcaaca ttgtgttgca gcggtggatt gctcaataga ttaacatatt ctatttcata
    25741 cccttcttct acgatggtac ctttgaccac gtcgggataa acaaatcggt aggtctgtga
    25801 atggtccgaa ttttt
//
LOCUS       DAOQGF010000040        25318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_905_length_25318_cov_7.633258, whole genome shotgun sequence.
ACCESSION   DAOQGF010000040 DAOQGF010000000
VERSION     DAOQGF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_25318_cov_7.633258"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PK937_07440"
     CDS             <1..1372
                     /locus_tag="PK937_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH32439.1"
                     /translation="RHNDGHRQNTEFDMVNLYGKTAYHFANNKNLQLAMNYNHVANDY
                     PATWLSFLQPYHVAPAKLDNRQQRSEFSTDLYYYSIPTSELKYSSRFYYYTNNSEYTF
                     WKDSSRMGNERIRQTSINNHRIGNVTQFDYYMSERHYMISGMEIQLDFVDARPANILY
                     GKHTAYNVAAYSQDEIKLSDRLTTTFGLRYDYNKIVDGYGEGNFSPKISTVYEIQKDW
                     SVRALVAQAFRNPSIAERYIKYEQGGNLHFHQNPNLKSEKLYASFEVGTKFKITDYTS
                     YDISLFWNEYKNMIAYIADTAVAYQGYYKVVNLNRARMRGVEASIHVFYKQYLNASAG
                     YTLLDARDISKDAGTRDVGYTSGNHDLPYKAKHSFNFNIDAGYEPVSLNLNGRYNSAI
                     REVSIYPGSEPDAFFVLNGKFSVEFLPKRSVYISAQNITNTQYEEIERYRMPGRSYMI
                     GCSMQF"
     gene            1379..1687
                     /locus_tag="PK937_07445"
     CDS             1379..1687
                     /locus_tag="PK937_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNH32440.1"
                     /translation="MKTRHRTNRISVIREFMQIPGVGKSIAQDFWDMGFRSVGELAKK
                     NPEKLYEDFCTRRGQIVDRCMLYVFRCAVYYALHPDPEPEKLKWWNWSDKKISSLSIH
                     "
     gene            1705..2832
                     /locus_tag="PK937_07450"
     CDS             1705..2832
                     /locus_tag="PK937_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH32441.1"
                     /translation="MKSTETSHDNARVKRLRSYHILDTESEQRFDDLARLASHICGTP
                     VAMITLIDSDRQWVKSSVGFPTVHLPRDVAFCSHTILGDELLIVRDASEDKRFEHHPL
                     VTGATQLRFYAGMPFFSFDGFALGSLCVLDNKPRDLSDEQKETLRALAHQIEDQLELR
                     RHLLNMQSQIDEKTMADEETRRREMYIRKILETSRDGVLVEENEKVVFMNTAYAKLFG
                     RTSADELMGLHLSELAAPHDLPRLLEYGRRRSLGEEVPSLYDFQIRNKNGDFVDVEAS
                     VSVFTHEDKKYIITFARDIMERKRHEKEREKLIAELQAALGEVKTLSGLLPICASCKK
                     IRDDKGYWNQLEYYIEANTKAMLTHGICPECVQKLYPEEDA"
     gene            3012..6434
                     /locus_tag="PK937_07455"
     CDS             3012..6434
                     /locus_tag="PK937_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32442.1"
                     /translation="MVSASKKRSLVFVFFSAIAGMMFGLAGLSVSVFSQQASFKRISI
                     EEGLSQSAVHCLLQDKLGFIWLGTQDGLNRYDGYGFKIFRHDPQDSLSISDNWIQTIY
                     EDRNGKLWIGTDGDGLNCYDRQTQSFVRYSARKGKPGSLPHGRVWAISEDVHGTLWVG
                     TIGGGLATLNQTTHTFTIYDTSNTSLASNEIMSLRATQDGKLWVGTFGGGLYRMDPQT
                     MQMQVYRHNPRNASSLADDRIRTIIEDQHGTLWIGTYNGLSILDRSDQEQGRFRSVQD
                     SVLTSRAVFALTENTDETVWVGTFGSGIRTIDRTGRIVSSYKYDAKDATSLGDNSLYA
                     FFCDRSGRMWIGTYSGGVNVFDTRKKDFRSMRHDPHNLASISDNRVRSIWKDTDGTMW
                     LGTFKGLNRLDSRTGYISFFTSESGASSSLPENRIRIIRRDTKGRLWIGTSAAGLAVM
                     DSLTNKISIYKHDPSSENSLPNNGVWSIAEDPQGTMWFGTTGGGFCRFDEKKKNFERV
                     QPASGEPGYFSNDPVLALHKGRDGKLWIGTASGLKSFVPTERRFIDLDFATNTPRSSS
                     LSRNRVRSIYEDHDGVLWVGTDVGLQRWDPDSSTLRLYNHRQGLISDVIYGILEDKQR
                     RLWLSTNYGIARFDPQSESFRNYDMTDGLANNEYNQNAYFRSHDGWFYFGGINGLDYF
                     HPDSIKDNTDKFAVVITGLELFNQTVEVGKSYNGFILPKAISELEELVLSYRESVIGL
                     KFSALTFTQPEKNRYAYKMEGFDEDWNYIDAGRRIATYTNLDPGTYRFQVRATNHDGI
                     WNENTCVLTIRITPPWWQTWWAYAVYTGLVVLGLMLFVRIRIQNERKRHELEKARELA
                     KINQELNDTIVRLRDTQAQLVQSEKMASLGQMTAGIAHEINNPLTFVYGNFEGLKDSV
                     EKMRRRYAEGSATIESTALPSPSETHGWMHELLEQINNGITGSRRIRDIVENLRKFAR
                     IGESGYKETDINADLLRIIDLFVKQHNDIRIDTSLGEGCIVYVNASEMNQVFFNVLTN
                     AVQAIQEAETQAVIRSGSGIICVQTASALAHDQAWISIVIKDNGVGIAPDDLKKIFDP
                     FFTTRPVGQGRGLGLSEAYGIVHKHGGHIDVSSEVGKGTSVRIVLPKGHHPV"
     gene            complement(6443..7003)
                     /locus_tag="PK937_07460"
     CDS             complement(6443..7003)
                     /locus_tag="PK937_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNH32443.1"
                     /translation="MHREAAWELLCEYTKGESLRKHALAVECAVRAYAVKFGEDVELW
                     SVTALLHDFDYEMFPDPVAPNGHPYKGNEILKDKGYPDDVRRAIMSHADYTGVSRDSL
                     LEKTLFACDELAGFITAVAYVRPTKSVKEVEVKSVRKKMKDKAFAAKVNRDDIINGAA
                     ALGIDLDEHIAFVIKALQDNADSLGL"
     gene            complement(7025..7999)
                     /locus_tag="PK937_07465"
     CDS             complement(7025..7999)
                     /locus_tag="PK937_07465"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNH32444.1"
                     /translation="MKTLKLKPTDANIRKAARILQKGGLVALPTETVYGLAANAMDAK
                     AVRRIFKAKGRPSDNPLIVHIADRRMLSTLVDTIPEHARLLMRHFWPGPLTLVLRHNG
                     TLPLTVTAGLSTVAVRLPGLALTRKIIRLSGVPLAAPSANRSGRPSPTQSEHVLHDLD
                     GRIEAVIEGGIARVGIESTVVDVTGKIPVILRPGHITIEDIRRVCDHARLSHHRRHTS
                     VRSPGMKYRHYAPDTPLQIITPEKIEAYAKRNTHLRLGIMTMRRGLHIPNATVIYIGN
                     TPTRAARHVFAALRKLDGKVDRIVCEALEEKGIGHAVMNRLKKAASKK"
     gene            complement(8000..9397)
                     /locus_tag="PK937_07470"
     CDS             complement(8000..9397)
                     /locus_tag="PK937_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNH32445.1"
                     /translation="MTSLYRPLGNTGYTCHPLGFGSYRINEDNPTHERALRSYIQHGG
                     NLIDTSANYGDGASETLIGKILSDINRSKMIVVSKGGYIQGQNLALAQKHDFPEVIKV
                     QDNLWHCIHPEFLETQITRSLQRLQSDYIDIYLLHNPEYFINHQSNFGPLDDTVHDAF
                     YRRVRRAFEYLESQVALGRIRYYGISSNNFGYHAQDRARTDVSRCWAMAEEVSPHHHF
                     RVVQMPMNPYEAGGAIYPTQNGLTPLEFCIKHNIGVLINRPLNAFHDNRLIRIADYVT
                     PGQNKPDISTLRDEMQPLINLENEFKLRFNGSPFGDEEQGLVSYLLYIADDLPSKEHW
                     NTVIDRYIVPPVTQWLRDQSALYSDSIVWNAWQENFVKAVNLTLDRVEKYVAYSDQKV
                     SDRVRQRLMDSGYPSSDASLSQMALSLLIQTQGVSCVLNGMRTESYVKDAMGALDLPA
                     VDAKHILERFDQSIR"
     gene            complement(9518..9979)
                     /locus_tag="PK937_07475"
     CDS             complement(9518..9979)
                     /locus_tag="PK937_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNH32446.1"
                     /translation="MKTIEEAFIHEGYLSIPFSYNGAGHPIIKVTVANGFQTNILLDT
                     GASANLLDLEFAQENGLNLTPTGEKGGGAGGLTLDVFTVDKLTLEINGKKLQFTDFLS
                     MDFTTIKQSLITNGLEADFQGILGFGFFKKTKCFIDYSSDRIFIQNNSYIE"
     gene            complement(10069..10743)
                     /locus_tag="PK937_07480"
     CDS             complement(10069..10743)
                     /locus_tag="PK937_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH32447.1"
                     /translation="MDKNSMTIKPGLAAPELRISEWIQGTPIQLSELQGRVVIIEVFQ
                     VNCPGCFLYGLPEILEIAAKHKNEPLTVIGLATAFEDFDINTKDNLQKLLQTGELVGE
                     TARVLKEHDWATDNKLRYTIPIPVAMDRLGEGSRLDTVSQIERIIERDITNFDRLSYG
                     EQGRIRESILKYLNERTLVPETFDLYNLSGTPSSIVIDKKGILRHIQFGSTGNLEKII
                     RPLLTE"
     gene            complement(10751..12040)
                     /locus_tag="PK937_07485"
     CDS             complement(10751..12040)
                     /locus_tag="PK937_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH32448.1"
                     /translation="MTIEPYFTTGQHNRFSEMAKGLLGSEILKIASDIRAMVAKGQKI
                     GNLTVGDFSPAEFRIPQFMEDAIRRHYAQGQTNYPPSDGMLELRNAIVGFYKTWLDVE
                     YPVKSVLVASGARPLIYSLFRAIVDPGDKVVYPVPTWNNNNFVQMLGAQGVPVHCHEE
                     HAFLPTVDLLKPHLHDARLICLNSPLNPSGTAFSRQELEAICDMILEENKRRTTEDAL
                     YLMYDQVYWMLTFGKTEHFNPVHLRPEIAPYTVMVDAVSKAFAATGVRVGWCVGPPDI
                     IERMSAILGHVGAWAPRAEQMATAELLNNKDEIVKYHSVMKVGVQDRLNALYEGLSAM
                     AKDGLPIKTIVPMGAIYLSVQFNFLGRKKPDGTVFTNSEEIRQYLLFESQIGVVPFKA
                     FGYQHDTGWFRLSVGAVSMKDIEEALPRLRRILEKLV"
     gene            12300..13163
                     /locus_tag="PK937_07490"
     CDS             12300..13163
                     /locus_tag="PK937_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNH32449.1"
                     /translation="MAVQTITVLGSGIMGNGIAHVAAQAGYKTILHDVSLDALNKALN
                     TISKNLDKGIELGKISAQDKEATLSRISVEQHLEAAVAPSDFVIEAVPEKIDLKIKIY
                     TTLDKTCKPETVFATNTSSLSITEIAAVTQRPQKCIGMHFFNPVHKMKLVEIIKALET
                     DEATIKATQDVAIAMGKEIVTIKESPGFITSRINALIGNEAFYMLQEGIGSAKDIDTA
                     LKLGLNHPMGPFEMVDLVGLDTRLSILEYLHKSLGEKYKPCPLMVQYVKAGRLGRKTG
                     KGVHEYPDVKK"
     gene            complement(13169..13648)
                     /locus_tag="PK937_07495"
     CDS             complement(13169..13648)
                     /locus_tag="PK937_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH32450.1"
                     /translation="MHPNEETINRFYRAFQNKDFSVMQHSYHDDAVFYDPVFEDLTAA
                     EVRTMWEMLCKRGKDLVLEYRIIHADDQRGSAEWIATYTFSATGRKVINRIHADFTFR
                     ENKIMSHKDTFDLYRWAKQAFGFSGVLLGWTGWMQNKIRSTAKNNLQRFMGKSSPVS"
     gene            complement(13732..15576)
                     /locus_tag="PK937_07500"
     CDS             complement(13732..15576)
                     /locus_tag="PK937_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32451.1"
                     /translation="MNLFFFAMMISASLWAQLKVAVVPFYNGTMLSKNEWLGHGFCES
                     LNTDLSEEKSVLIMDRPDVLAEIKMNGFKLLDMLDEQKALTVARSLEVDKIIIGSFDI
                     PGGATPSIKVQARFIDVKSGKADTKNAYTAEGKYSMTNIYAMYGQIAARALVSFGKRA
                     ADETAVTGKGSINVDAYEPYIKGILQYDESSTVEDYLASIALLQQAIKTDSSFAMAYA
                     GMAKAYAKIGRIQDINFKADEKTESYKKALDAGLKAAKLDKNLAVAWTALALTYRELK
                     ERDQLIEAARKAVALKPSQYDAYDMLADAFSANFFPAHKNNDSSVFYRKKSVEYNNKF
                     ASGYRGLGRDYFDKGDYKNAEEAYQRAITLNPKHAGSRDLLGQVYTIQGQYEEAKQQF
                     REAIKLDAKSAFAHTHLADVLAMEKNYAEAVTSYQSAIAINAKFALAYNGLAWIYIAA
                     KDKTLRIPAKAVENAQAAVQHSDSKNASYLYTLSEAHFSAGTHDAALEAIGKAIALDP
                     HNNDYAETQSRITKKEKNSDYVFTLRRGMMLMKQGRQDEGVVEFEEANKLSPQNVYIL
                     FTLAKYYDSKKEFKKAFEYYYRARSCDWDKKYSKSIQERMNELAPYGN"
     gene            complement(15637..17019)
                     /locus_tag="PK937_07505"
     CDS             complement(15637..17019)
                     /locus_tag="PK937_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32452.1"
                     /translation="MKSRFDFFITVLCVCGVVTLSAQSASDLVQQANEKFSSAGSEKE
                     YKEAAGIYEKAAAADSTLAIAWAGLAKSHAMIGNIASLNYKDDLKIKHYKESAKYASK
                     AGKMAKENSDVQTALAMVYRQLSQRDNLMRVAQKAVALDPKNAEAYDLWADAYSEDYF
                     PKNADIDSSILIRKKAVEANPNFAKGYRGLGDDYASKGDLEQAESYFTKAIAINPQHA
                     GSHDRLGRLYYLQGEFEKAKTEFNTAIKLDPRSTFAYTHLADVLADEGNFKEAFLKFT
                     YAVDLNPNAAYAHASLSWMYLTAKDKSFRNVGKAIEYGAMAAELTNNTNPEFLAVAAE
                     AYYEASFNKSELRDSAINRLKTALSIEPDNFEYQETLGRMNAKEKSREYVYAWKRGER
                     LLKHNRINDAINEFITSEEKNDKFVPNLISYAKALKKKGDEATMKVYLEKARKADKSG
                     KYTTSISSVQ"
     gene            complement(17187..18527)
                     /locus_tag="PK937_07510"
     CDS             complement(17187..18527)
                     /locus_tag="PK937_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNH32453.1"
                     /translation="MSEESKVNRFFSRPVMIGIFSILLFISALLFVRDPLFQQSSNGQ
                     WLFLSIPVEFILFGLTLLCVALFHHYVLQVALTGLAVILTYKLSFTSFDVVAHIHHEW
                     AIVVNLLGLLVGFAILAKHFEESHIPEILPKHLPNGMAGPLVLLCITFVLSSFLDNIA
                     AAMIGGTIALTVFRGNVHIGFLAAIVAASNAGGAGSVLGDTTTTMMWIDGVSPWDVLH
                     AYYGSIGAFAFFAVVASIQQNKLQTIIRAEGSENHSIDWGKILVVALILVGAIATNIL
                     LDFPALGVWAAIFIGAFIRETAWHEIPVALKGSVFLVSLVLSASMMPVDQLPPASWFS
                     AFILGFVSSVFDNIPLTKLALDQGGYDWGVLAYAVGYGGSMIWFGSSAGVAISGIFPK
                     AKSVVFWLKDGWHVAVGYVIGFFIMLALWGWEPHEKHKIEKGESHKQPSTEQTH"
     gene            complement(18574..18699)
                     /locus_tag="PK937_07515"
     CDS             complement(18574..18699)
                     /locus_tag="PK937_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32454.1"
                     /translation="MKTHTQLTTETKPKKDYLFVSLMALIGLSILAFILRLAGLI"
     gene            complement(19081..19431)
                     /locus_tag="PK937_07520"
     CDS             complement(19081..19431)
                     /locus_tag="PK937_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32455.1"
                     /translation="MMRTFLHTAFSKALILTYIFIGYLAITTTDKPQCVIYAQHVMQT
                     DPTTVPSSVTIKVFAARHIQPEKLSRSIAYVSLFDFVIPPACATLLRVVKSTHFTSYN
                     GFSIEQPARAPPIA"
     gene            complement(19565..19933)
                     /locus_tag="PK937_07525"
     CDS             complement(19565..19933)
                     /locus_tag="PK937_07525"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH32456.1"
                     /translation="MKLRWFISVMTVLIFGSWYGFVSCQTNRPKNIKVLDELKDATIE
                     EIKESMRLYTLSMGVECDYCHNKDDYASDEKKEKNISRDMMRMVRTLNESTFKNAKEN
                     VTCYTCHRGAVKLKNIPPGL"
     gene            20125..21252
                     /locus_tag="PK937_07530"
     CDS             20125..21252
                     /locus_tag="PK937_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH32457.1"
                     /translation="MLPDFDASQFMIEPLERASTIPSSWYTDARFHAVDREFIFSKSW
                     QYVGHVSQVAEAGQYLTATVADNPVVVLRDTENHLRGFYNVCRHRGGPLVTDARGTCN
                     KVLQCQYHGWTYKTDGSLRGIPRWNYVDLFDKKDYGLVPLQVDVWGGLVFVSLEKEPM
                     PLTKLFQGAQERISRKAYDADLERRLFSDLTFYKRIVYPVHANWKVYADNYLEGYHLP
                     FVHPELTSMLDYAQYVTETYEYYSIQYSPFSEEGNVYKTNEGEALYYFVWPNFMLNIL
                     PGRLQVNRIIPDGHERCHVIFDYFYADLSTEAKRAMAEDDIAYSDKVQKEDCDICEHV
                     HKGLMSDVYDRGRFSVECENGVYHFQNLLKKNYQSVSQDNR"
     gene            21274..22596
                     /locus_tag="PK937_07535"
     CDS             21274..22596
                     /locus_tag="PK937_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNH32458.1"
                     /translation="MTEKKELAQSIALWDAIMLNAGSMIGSGIYIVPAAVAGYLQGSV
                     WIMTAWVVGGLVSMLGALCLAELGAMMPKAGGIYIYLSRAFSPMWGFLYGWANFAVIM
                     TGSIAAVAVGFATYLGYFIPFTPDEIKAVAIASIIFLTAINVVGILFGVAVQNFMTLL
                     KIVSLGILIIIPIVVPGGSVENFTPLFSDPNSGHWIGAFGLATIAVLWAFDGWIEITY
                     VAGEVRDPQKNVPRALVVSTALIIGLYVLINMAYLYVLPADQLRASSLVASDMAKILM
                     GPEGAAFVAGSVMISMFGANNGFVLSGARIYYAMAREGRFFEKIGDVHPRFGTPAVSI
                     IAQGVWSCGLVFTGTFEQLTTYVVFASWFFYAMACMAVIVLRKKDPQTERPYRAWGYP
                     WTPIVFIAFAAFLIGNAVMEDLRSSLIGVGIIITGLPLYGYWNQHRSR"
     gene            22565..24781
                     /locus_tag="PK937_07540"
     CDS             22565..24781
                     /locus_tag="PK937_07540"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNH32459.1"
                     /translation="MGIGINIEVGNFIIFQSILSKKSFMRFTLIVLALLLWCSPIYAQ
                     NENPADQRIDSLKEAFEREPSSQAIMEALGRAYAETQSYEAIIDLIKPVIEDTSTNNV
                     LIKLYVGARIKQLQTQSAGPKLLQQLLGGADTKEKKLINRVMQRDPEDAEAQLFLAEL
                     HKLADEPVYAENIYRRMMKNPRVISGPWFVSVWTAYSTLLIDQERFTEANSILTHAAG
                     TEKSSGWFELQMAQVTLETGETARGTRLFFQGLDALRDESQVHQLFEEAWAIAARTEL
                     EQWNTLNTLDAKKDFLRRFWSKRTPNLADSANAKLAENYKRLRHVKRYYARRTFPGYD
                     ERGLVFMRMGKPDHFYTDTRTESWVYESANAHFDFVDPGSGYYEMRPLTDALSLSGSA
                     AGQFQELKAMMEARRNLHPFYEQTAARLQAREDAISVERSRSNAFRNIISETEIRLGR
                     EGTNKPGLQKSETLDKIPSVSLVASYACFKGKNKSTRVDFYYMIPIRELQTLRIADSS
                     STAFLFANARILDTNYREITTLHREFKIAVVDRDAPLFVIDELRTLLQPGTYILGLEL
                     INNNNERKGVYRFDVEVRDFIGANLSISDIQPAVSVTVGLLEDKFIKPDTRIRVVPFA
                     GDHFDGRKPLSVYYEINNLTRDLKGVTSYEISYTLAPAQRGNILTRAVGKLFGSSKAT
                     ITSVTQKTGTLPTEREYVGFDISELPEGECVLTITVKDLLSGQTASSERFFWVNKK"
     gene            complement(24839..>25318)
                     /locus_tag="PK937_07545"
     CDS             complement(24839..>25318)
                     /locus_tag="PK937_07545"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNH32460.1"
                     /translation="DDQKIFEFLILEGFQAGLSWITILRKRDNFRKAFKQFDPKKVAR
                     FSKADVIRLMQDTGIVRNRLKIEGAINNAKCFLEVQKEFGTFDRYIWSFTGYKTLRNP
                     KRSRENIMATSPESDALSADLKLRGFKFVGSTVIYAHMQASGMVDDHVKGCWKHKKR"
BASE COUNT         6699 a         5424 c         6162 g         7033 t
ORIGIN      
        1 ccgccacaac gacggtcatc gtcaaaatac cgaatttgat atggtcaatc tgtacggtaa
       61 aacggcgtat cattttgcta ataataaaaa tctgcagctc gcaatgaatt ataatcatgt
      121 ggctaatgac tatccggcca cgtggctcag ttttttacag ccgtatcatg tcgcaccggc
      181 aaaactggat aatcgccaac aacggagcga atttagcaca gacttatatt attattcgat
      241 accgacttct gaattgaaat actcatcgcg attctattat tacacgaata attccgagta
      301 cacgttttgg aaagattcga gccgaatggg taacgaacgt attcgccaaa cttcgatcaa
      361 caatcatcgt atcggtaatg taacacagtt cgattattat atgtccgaac ggcactacat
      421 gatttcaggt atggaaattc agttggattt tgttgacgca aggccggcca atatattgta
      481 tggcaaacat acagcgtata atgtagccgc atattctcaa gatgaaatca aactcagcga
      541 tcgcctcaca acgacattcg gtcttcgcta tgattacaac aaaattgtcg atggatatgg
      601 cgagggtaat tttagtccta agatttcaac tgtatatgaa attcaaaaag actggtcggt
      661 tcgtgcattg gtagcgcagg cgtttcgtaa tccgtccatc gccgaacgtt atatcaaata
      721 cgaacagggc ggtaatttgc attttcatca gaatcctaat ctcaagtccg aaaaactcta
      781 cgcgtcgttt gaagtcggga ccaaatttaa aatcacggat tatacatcgt acgatatttc
      841 gctattctgg aatgaataca aaaacatgat cgcgtatatc gccgatacgg cggtggcgta
      901 tcaaggatat tacaaagtcg tcaatctcaa tcgtgcccgc atgcgcggcg tcgaagcgag
      961 catccacgtt ttttacaaac agtacctcaa tgcttcggcg ggatatacat tacttgatgc
     1021 gcgggacata tccaaagatg cgggaacccg cgatgttggt tatacttccg gcaatcatga
     1081 tctgccgtac aaagccaaac attcattcaa ttttaacata gacgctggct acgagccggt
     1141 cagtcttaac ctcaacggtc gttataacag tgccattcgt gaagtttcca tttatcccgg
     1201 gagcgaaccg gacgcttttt ttgttttgaa tggcaaattc tccgtagaat ttttacccaa
     1261 acgcagtgtg tatatttcag cgcaaaacat tacgaatacg caatacgaag agatcgagcg
     1321 gtatcgtatg cccgggcgga gttatatgat cgggtgttcg atgcaatttt gatcggttat
     1381 gaaaacacga caccgtacga acagaatatc agtaatacgc gaatttatgc aaatcccagg
     1441 ggtgggcaaa agtatagccc aagacttttg ggatatgggt tttcgttcgg taggcgaatt
     1501 agccaaaaaa aatccagaaa aactgtatga ggatttttgt acacggcgtg gtcagatcgt
     1561 agatcgctgt atgttatatg tttttcgctg cgccgtttat tatgcattgc acccagatcc
     1621 ggaaccggaa aaattgaaat ggtggaactg gagtgataaa aagatttctt ccctctcaat
     1681 acattaacat cgtatattgc tacgatgaaa tccacagaga cctcccatga taacgcacgc
     1741 gtaaaacgtc tccgttcgta tcatatcttg gatacggagt cggaacaacg ttttgatgat
     1801 ttggcacgtt tggcttccca tatctgcggg acgccggtcg cgatgatcac attgatagac
     1861 agcgatcgcc agtgggtcaa atcatcggta ggttttccga cggtacacct accgcgtgat
     1921 gtagcgtttt gttcgcacac gattcttggc gacgaactgc ttatcgttcg cgatgcttcc
     1981 gaagataaac gtttcgaaca tcatccgttg gttaccggtg caacgcaact tcggttttat
     2041 gccggcatgc cgtttttttc gtttgacggt tttgcgctgg gttcactatg cgttttagat
     2101 aataagccgc gcgatctgag cgatgaacaa aaagagactt tacgtgcatt ggcgcatcag
     2161 atagaagatc aattggaatt acgccggcac cttctgaata tgcaatcgca gatagatgaa
     2221 aaaacgatgg ccgacgaaga aacgcgtcgc cgtgagatgt acattcgtaa aatattagaa
     2281 acttcacgag acggagtttt agtagaagaa aacgaaaaag ttgtttttat gaatacggcc
     2341 tatgcaaaat tgtttgggcg aacaagcgcg gatgaattga tgggtttaca cctttcggaa
     2401 ttggccgcgc cgcatgatct gccgaggctt ttggagtatg gtcgccgtcg ttcgttgggc
     2461 gaagaagtgc cttcgttgta tgatttccag atacgtaata agaacggcga cttcgtagat
     2521 gtcgaagcat cggtatctgt ttttacgcat gaagataaaa agtatatcat cacgtttgcg
     2581 cgtgatatca tggaacgtaa gcgtcatgaa aaagaaagag aaaagctgat tgccgaattg
     2641 caagcggcct taggcgaagt taaaacgcta agcgggcttc tgccgatatg tgcatcgtgt
     2701 aaaaaaattc gtgatgataa aggctattgg aatcagttag agtattatat cgaagcgaac
     2761 acaaaagcta tgttgacgca tggcatctgt cccgagtgtg tacaaaaatt gtatccggaa
     2821 gaagacgcat agataatagg ttctctcctc cgtgcgcatg ccgtgataag gcattaccct
     2881 caaagtgatt taaaaaaact atatgcaaaa tgtagtagcg atacttattg gtttgaatta
     2941 agttaagcct ccttggcttt cggttttcat ttaatcacca gtatttcctt ttactcatac
     3001 gggttttatt gatggtctct gcttccaaaa aacggagttt agtttttgtt ttcttttctg
     3061 ccattgcagg aatgatgttc ggtttggcgg gtttatccgt ttctgttttt agtcaacagg
     3121 cctcgttcaa gcgtatttcc atcgaagaag gtttatcgca aagcgccgtc cattgtcttt
     3181 tgcaagataa actggggttc atatggttgg gtacccaaga tgggcttaat cgttatgatg
     3241 gatacggttt taaaatattt cgtcatgatc cgcaagacag tctttcgatt tcagataact
     3301 ggattcaaac aatctatgag gatcgaaacg gaaaattatg gataggtacg gatggcgacg
     3361 ggcttaactg ttatgacagg caaacccaat catttgtccg gtattcggcg cgaaaaggca
     3421 aacccggttc gcttccgcac ggtcgtgtat gggccatctc cgaagatgtt cacggcacat
     3481 tatgggtcgg aacgattggc ggtggtctag cgacgcttaa tcaaactacg catactttca
     3541 cgatttatga tacgagcaat acttcacttg catccaatga aatcatgagt cttcgtgcga
     3601 cgcaggatgg aaaactatgg gtcggtactt ttggcggtgg attgtatcgt atggatccgc
     3661 aaaccatgca gatgcaggtg tatcgccata atccccgcaa tgcgtcatca ttggcggatg
     3721 atcgcatacg cactattata gaagatcaac atggtacgtt gtggattggt acgtataacg
     3781 gtttgagtat tcttgatcgt agcgatcagg agcaaggacg ctttcgatcc gtgcaggatt
     3841 cggtcttgac atccagagcc gtttttgcat taacagaaaa taccgatgaa acggtatggg
     3901 tcggaacatt tggcagcggt attcgcacga ttgatcgtac gggccgtatt gtgagttcgt
     3961 acaaatatga tgcgaaggat gctacaagtt taggtgacaa tagcctctat gcgttttttt
     4021 gtgatcgctc agggcgtatg tggataggaa catatagcgg cggagtgaat gtttttgaca
     4081 cacggaaaaa agattttcgt tcgatgcgtc atgaccctca taatttggca tccatttcgg
     4141 ataaccgtgt tcgtagtatt tggaaagata cggatggaac gatgtggctc ggaacattca
     4201 aagggttaaa tcgtttggat tcgcgcacag gatatatttc tttttttacg tcggaatcgg
     4261 gcgcatcatc atcattaccg gaaaaccgta tccgtatcat tcgccgtgac accaaagggc
     4321 gtttgtggat agggacaagc gcagcgggat tggctgtcat ggactcattg acaaataaga
     4381 tttcgattta caaacatgac ccctcgtcag aaaactcttt accgaataac ggcgtttggt
     4441 ccatcgctga ggatccacaa ggtactatgt ggtttggtac gaccggaggc gggttttgtc
     4501 gttttgatga aaagaagaaa aattttgaga gagttcaacc ggcctcgggt gagcccggtt
     4561 atttttccaa tgaccctgta ctggcattac ataaaggccg tgatggcaaa ctttggattg
     4621 gcactgccag cggattaaaa tcatttgttc ccacagagcg ccggtttata gatttggatt
     4681 tcgcgacaaa cacaccgcga tcctccagtc tctcacgaaa tcgtgttcgt tcgatctatg
     4741 aagatcatga cggtgttttg tgggtcggta cagatgtcgg tttgcaaaga tgggatccgg
     4801 attcgtctac gctgcgacta tacaatcacc gacaaggttt gatcagtgat gttatatacg
     4861 ggatccttga agataaacaa cgtcgtcttt ggctaagtac caattacggt atcgcacgat
     4921 ttgatcctca atcggagtca tttcgtaact atgatatgac cgatggttta gctaataatg
     4981 aatacaatca gaatgcgtat tttcgttcgc acgacggatg gttttacttc ggaggaatta
     5041 atgggttgga ttattttcat ccggacagca tcaaagataa tacggataaa tttgccgtcg
     5101 tgatcaccgg cctggagtta ttcaatcaaa cggtagaagt gggaaaatcg tataacggat
     5161 ttatacttcc caaagcgata tccgaattgg aagagctcgt cttatcctat cgcgaatcgg
     5221 taatcggatt aaaattctct gcattgacat ttacgcaacc tgaaaaaaac aggtacgctt
     5281 ataaaatgga aggctttgac gaagattgga attatattga tgccgggcgt cgtatcgcaa
     5341 cgtacaccaa tctggatccc ggtacgtacc gattccaagt gcgtgcaact aaccacgacg
     5401 ggatatggaa cgaaaacaca tgcgttctga ctattcgtat cacaccacct tggtggcaaa
     5461 catggtgggc ttatgcggtt tatacgggtt tggttgtgtt aggcctaatg ctttttgttc
     5521 gcatacgcat tcaaaatgaa cgtaaacgtc atgaactgga aaaggcacgt gaactggcca
     5581 agatcaatca agagctaaat gatacgatcg tgcggcttcg ggacacgcag gcgcaactcg
     5641 tacaatctga aaaaatggct tcgctcggtc agatgacagc cggcatcgca cacgaaatca
     5701 ataatccgtt aaccttcgtt tacggtaact ttgaagggtt gaaagacagt gttgaaaaaa
     5761 tgcgtcgtcg gtatgctgaa ggtagtgcta cgatcgaatc cacagcgctt ccttctccgt
     5821 ccgaaacgca cggatggatg catgaactat tggagcaaat aaacaatggt attaccggtt
     5881 cgcgccgcat ccgtgatatc gtcgaaaacc tgcgaaagtt tgcccgcatc ggtgaaagcg
     5941 gttacaaaga aacggatatc aatgcggatt tactccgcat cattgatttg tttgtcaaac
     6001 agcacaacga cattcgcatt gatacctcac tgggcgaagg ttgtattgtt tatgttaacg
     6061 catcggaaat gaatcaggtg ttttttaatg tattgaccaa tgcagtgcag gcgatacagg
     6121 aagctgaaac gcaggcggtg attcgctctg gttccggtat tatttgcgta caaacggcat
     6181 cagctttggc gcacgatcag gcgtggatat ctatcgtcat caaggataat ggtgtcggta
     6241 ttgcacccga tgatcttaag aaaatatttg acccgttttt tacgacgcgc ccggtaggtc
     6301 aggggagggg attaggttta tcggaggcat acggtatcgt tcacaaacac ggcggacata
     6361 ttgatgtgtc atcggaggtt ggaaaaggca catccgtgcg cattgtttta ccaaagggtc
     6421 atcaccccgt ttaatacatc cgctataatc ccaacgaatc cgcattatct tgcagggctt
     6481 taataacaaa agcgatatgt tcatccagat ctatgccgag cgcggccgct ccgttgataa
     6541 tatcatcgcg attgactttg gccgcgaagg ccttgtcttt catcttcttt cgaacggatt
     6601 ttacttcgac ttcttttacc gactttgtgg gacgaacata cgcaacggcg gttatgaaac
     6661 cggccaattc atcacaagca aagagagttt tttcaagcaa agagtcgcgc gaaacaccgg
     6721 tataatccgc gtgggacatg atcgcacgac gcacgtcgtc cgggtaacct ttgtctttga
     6781 gtatttcgtt tcctttgtag ggatgtccgt ttggtgcaac cggatccggg aacatctcat
     6841 aatcaaaatc atgcaatagc gcggtgacac tccacaactc aacatcttcg ccaaatttta
     6901 cagcataagc ccgaactgca cattctacgg ctaaagcatg tttacgaagg ctttcgcctt
     6961 tggtgtactc gcacaaaagc tcccaagctg cttcccgatg catgatttat ctccgtcgta
     7021 taatttattt tttactcgct gcttttttca ggcgattcat cacggcatgg ccgatccctt
     7081 tttcttccaa tgcttcacac acaatacgat ctacttttcc gtctaatttt cgcaatgcag
     7141 cgaaaacatg ccgcgcagcg cgtgtcggtg tgttgccgat ataaatcaca gtggcattgg
     7201 gtatatgcaa accgcgacgc atggtcatga taccaagtcg aagatgcgtg ttgcgtttag
     7261 cataggcttc gattttttcg ggtgtgatga tttgtagagg tgtatccggt gcataatgac
     7321 ggtacttcat acccggtgaa cgtacgctgg tatgccgacg gtggtgtgac aaacgggcat
     7381 gatcgcacac acgacgtata tcctcaatcg tgatgtggcc cgggcgaagt atgaccggaa
     7441 tttttccggt gacatctacg actgtagatt cgataccgac gcgggcaatg cccccttcga
     7501 tgacggcctc aatgcgtccg tcaagatcat gcaagacatg ttcggattgc gtagggcttg
     7561 gtcgcccgga gcgattggcc gacggcgcgg cgaggggtac gccggataag cgaatgatct
     7621 ttcgtgtcaa agcaagtccc ggtaaacgta cagcgaccgt ggataaaccg gcagttacgg
     7681 tcaagggtag tgtaccattg tgtcgaagta caagtgtaag cggtcctggc caaaaatgac
     7741 gcatgagtaa ccgcgcgtgt tccgggatcg tgtccaccaa agtactgagc atccggcgat
     7801 cggcgatgtg tacgatcaat ggattatcag agggtcttcc tttcgctttg aaaatacgcc
     7861 gaacagcctt ggcgtccatc gcattggctg cgagaccata caccgtctcc gtgggcagtg
     7921 cgaccaaacc gcctttctgt aggatgcgcg cggctttacg gatatttgca tccgtaggct
     7981 tgagcttgag cgttttcatt tagcgaatgg attgatcaaa gcgttctaaa atatgtttgg
     8041 catccaccgc aggcaaatcc agtgcgccca tagcatcttt gacgtacgat tcggtgcgca
     8101 tcccgttgag tacacaagag acgccttgcg tttgtatgag cagtgacaat gccatttggc
     8161 tcaacgacgc atcacttgac ggataaccgg agtccatgag ccgttgacgg acgcgatcgg
     8221 aaactttctg atcgctgtag gcgacatact tttcgacgcg atccaatgtg agattgacgg
     8281 ctttgacaaa attttcctgc caagcattcc aaacgatact atcgctgtag agtgcacttt
     8341 gatcgcggag ccattgggta accggcggta cgatgtagcg gtctatcacc gtattccaat
     8401 gttctttgga tggaagatca tcggcgatat acagcaaata agaaacaagg ccctgctctt
     8461 catcgccgaa cgggctaccg ttgaagcgca gtttaaattc gttttcaaga ttaatcaggg
     8521 gctgcatttc gtcgcgaagc gtggaaatat ccggtttatt ttgtccgggt gtcacatagt
     8581 cggcgattcg aataagtcgg ttatcatgaa aagcgttcag aggacgattg attaaaacac
     8641 cgatgttgtg tttgatgcaa aattccaacg gcgtcaaacc gttttgcgtg ggatagatgg
     8701 cgcctcctgc ttcatacggg ttcatcggca tttgtaccac acgaaaatga tgatggggtg
     8761 aaacttcttc tgccatcgcc cagcagcgcg atacgtccgt gcgtgcgcga tcttgcgcat
     8821 gataaccgaa attattggag ctgatgccgt agtatcggat acgccccagg gcaacctgcg
     8881 actccaggta ttcgaaagct ctgcgcacgc gacgataaaa cgcgtcatgt accgtatcgt
     8941 caagcggacc gaaattggat tgatgattga taaaatattc cgggttgtgc aggagataga
     9001 tgtcaatgta atcactttga aggcgttgga gagagcgggt aatctgtgtt tccaaaaact
     9061 caggatgaat gcagtgccag agattatcct gcactttgat gacttccgga aaatcatgct
     9121 tttgcgctaa ggcaaggttt tgcccttgaa tgtatccgcc tttggaaacc acgatcattt
     9181 tgctgcgatt gatatcgctg agaatttttc cgatcaatgt ctccgaagcg ccatcgccat
     9241 aattggcgct ggtatcaata agattgccgc cgtgctggat atacgaacgc aaggcacgtt
     9301 cgtgcgtcgg gttgtcctcg tttatccgat aactgccgaa acccaaagga tggcaagtat
     9361 atcctgtgtt accaagaggc ctgtaaagtg aagtcatggg taaatgtatg gggtgatatg
     9421 tttgatgata tagcctacaa acatcatgat atgtttagct tcaaggaatt caatcagtcg
     9481 aagtatatcg aaaaaaaacg gtaaatcgaa ggctatatca ttcgatataa gaattgttct
     9541 gaataaaaat tctatctgaa gagtaatcta taaaacattt agttttctta aaaaatccaa
     9601 aaccaagaat accctgaaaa tcagcttcta aaccgttagt tatcaatgat tgttttattg
     9661 tcgtaaaatc catcgaaaga aaatcggtaa attgaagttt tttgccgttg atttctagtg
     9721 taagtttgtc aacggtgaat acatccagcg tgagtccgcc ggcaccgccg cctttttcac
     9781 cggtcggtgt cagattcagt ccgttttctt gtgcgaattc taagtcaagt aaattagcag
     9841 aggcgcccgt atcaagcaga atgttggttt gaaaaccatt cgcaacagta actttgataa
     9901 tagggtgtcc ggcgccattg taagaaaaag gaatacttaa gtatccttca tggataaatg
     9961 cttcttcaat ggttttcatt gttttattcc aatgagtttt tgacagaatt gattctctat
    10021 tttaaaattt gatctgttat tagaagaaga ttttataatg tgagattttt attcggtcag
    10081 caacggccga atgatttttt ccagatttcc tgtcgagccg aactggatat gacggaggat
    10141 gcctttttta tcaatgacaa tactggaggg agtgccgctg agattgtaga gatcaaatgt
    10201 ttcgggaaca agtgtgcgtt cgtttaagta tttcaatatg gattcgcgga tacgtccttg
    10261 ttccccgtag ctgaggcgat cgaagttggt gatatcgcgt tcgatgatgc gttcgatttg
    10321 tgaaaccgta tccaagcgtg aaccttcacc caggcgatcc attgcgacgg gaatgggaat
    10381 cgtgtaacgc agtttgttgt cggttgccca gtcatgttcc ttcaagacgc gtgccgtttc
    10441 accgaccaat tcaccggttt gtaaaagttt ttgaagattg tctttggtat tgatgtcaaa
    10501 atcctcaaac gccgttgcca gaccgatcac ggtaagcggt tcgtttttat gtttggcggc
    10561 gatttccaaa atttcgggta aaccatagag aaagcagccc gggcaattga cctgaaatac
    10621 ttcgatgatt acgacgcgcc cttgcaattc ggaaagttgg attggtgtac cctgtatcca
    10681 ttcggaaatg cgcaattcgg gcgccgcaag accgggtttg atggtcatac tgtttttatc
    10741 cataaaccgg tcagactaat ttttctaaga tacgacgcaa tcgcggcaac gcctcttcga
    10801 tatctttcat ggaaacggcg ccaaccgata agcgaaacca tcccgtatca tgctgatatc
    10861 cgaaagcttt aaacggtact acgccgattt gtgattcgaa taaaaggtac tggcggattt
    10921 cttcgctatt ggtgaatacg gttccgtccg gttttttgcg gccaagaaaa ttgaattgta
    10981 ccgaaagata aattgcgccc atgggtacga tcgttttgat gggtaaaccg tccttggcca
    11041 ttgcggacag accttcgtat aaagcgttga gccgatcctg cacacctact ttcatgacac
    11101 tgtggtattt gacgatttca tctttattgt tgagaagttc ggcggtggcc atttgttccg
    11161 cacgcggtgc ccacgcgccg acgtggccga gtatggcgga catacgttcg atgatatccg
    11221 gaggaccgac gcaccatccg acacgcacac cggtagcggc aaacgctttg gaaaccgcat
    11281 ccaccatcac cgtgtaggga gcgatctccg ggcgaaggtg taccgggttg aagtgctctg
    11341 ttttaccgaa agtcaacatc caatacacct gatcatacat cagatacaaa gcgtcttcgg
    11401 tagtgcggcg tttattttct tcaaggatca tgtcgcagat ggcctcaagt tcctgacgtg
    11461 aaaacgccgt gccggacgga ttgagaggtg aattgagaca tatcaggcgt gcatcgtgca
    11521 aatgcggttt cagtaaatcc accgtcggta aaaacgcatg ttcctcatga cagtgtaccg
    11581 gaacgccctg cgcaccgagc atttggacga aattattgtt attccatgtc ggaacagggt
    11641 aaacgacttt gtcgcccggg tccacgatcg cacggaaaag gctgtagatc aatggtcgtg
    11701 caccgctggc aacgagaacg gacttaaccg gatattctac atcaagccat gttttataaa
    11761 agccgacgat tgcgttgcgg agctcgagca tgccgtccga aggaggatag ttggtttgtc
    11821 cttgggcata atgccggcga atggcgtctt ccataaactg aggaatacga aattccgccg
    11881 gactgaaatc gccgacggtg agatttccga ttttttgccc tttggccacc atagcgcgaa
    11941 tgtcggaagc gattttgaga atctccgatc cgagaaggcc tttggccatt tctgaaaaac
    12001 gattgtgttg acctgtcgta aaataaggtt cgattgtcac tgtgaaaact cctttttaag
    12061 ccgcggcaaa atatataaat aggcattgaa atgctatcat aaaaaacgcc ttatcacgag
    12121 ataatatatc acgtgccgca tcgccggggt atgtcggaag tcatgcggga taataatgag
    12181 atgcagatgt agtgtgccgg tatgacagtc aatatccaag atattttatt tgcttccgtt
    12241 gcaaagctgt tctattattg caccacttaa aaaacatttt acagaggaga ttataaacga
    12301 tggctgttca aacgatcact gtactcggat ccggtattat gggcaacggc attgcgcatg
    12361 tcgccgcgca agcgggatat aaaaccattt tacatgatgt gtcgctggac gcattgaata
    12421 aagcgctgaa tacgatttcc aaaaacctgg ataaaggtat cgaacttgga aaaatctcgg
    12481 cgcaggataa agaggcgacg ctcagtcgta tatccgtcga acagcatctt gaagccgccg
    12541 tcgcgccgtc ggatttcgtt attgaagcgg ttccggagaa aattgattta aaaatcaaaa
    12601 tttatacgac gctggacaag acctgcaaac cggaaacagt atttgcaacg aatacgtctt
    12661 cgctgagcat tactgaaata gcggccgtga cacaacgccc gcaaaagtgt atcgggatgc
    12721 atttttttaa tccggtacac aaaatgaaac tggtcgaaat cattaaagca ttggaaacgg
    12781 atgaggccac cataaaggct acgcaagatg ttgcaatagc gatgggtaaa gaaatcgtaa
    12841 cgatcaaaga gtcgcctgga tttattacca gtcgtatcaa tgcactgatc ggcaatgagg
    12901 cgttttatat gctgcaggaa ggtatcggtt cagccaaaga tattgatacc gcattaaagc
    12961 tcgggctcaa ccatccgatg ggaccttttg aaatggttga tttagtcggt ttggatacgc
    13021 ggctttcgat tttagagtat ttgcataaat cgctcggtga gaaatacaaa ccttgccctt
    13081 tgatggtgca gtatgtcaaa gcaggacgac ttggtcgcaa aacaggtaag ggtgtgcacg
    13141 aataccccga tgtgaagaag taatatttct aagaaaccgg gctacttttc cccatgaatc
    13201 gttgaaggtt gttttttgcg gtggagcgta ttttgttttg catccaaccg gtccaaccta
    13261 ataaaacacc ggaaaaacca aacgcttgtt ttgcccagcg gtaaagatca aacgtgtctt
    13321 tgtgcgacat gattttattt tcgcggaaag tgaaatcagc gtgaatacga ttgatcactt
    13381 ttcgcccggt agctgaaaat gtatatgttg caatccattc cgcggagccg cgttggtcgt
    13441 cggcatgaat aatgcgatat tccaagacca gatcttttcc gcgtttacaa agcatctccc
    13501 acatggtccg cacttctgcc gccgtcagat cttcaaacac cggatcgtaa aaaacggcgt
    13561 catcatgata gctatgttgc atgacggaaa aatctttatt ttgaaatgct cggtagaatc
    13621 ggtttatggt ttcttcgttg gggtgcatag atctagttct tttcttatgt attatattaa
    13681 aaaagctgcc cgatgttttc aggcagcttt tttaattaaa tatcctgttt tctaatttcc
    13741 gtaaggtgca agctcattca ttcgttcctg aatggatttc gaatattttt tgtcccagtc
    13801 gcaactgcgc gcacgatagt aatattcgaa cgcttttttg aattcttttt tgctgtcgta
    13861 atatttagct aacgtaaaaa gtatgtagac gttttggggg ctgagcttgt tcgcttcttc
    13921 aaattcgacg acgccttcgt cctgccggcc ttgtttcatc agcatcatac cgcgtcgcag
    13981 tgtaaaaaca taatcgctgt ttttttcttt tttggtaatg cgactttggg tttcggcgta
    14041 gtcgttatta tgcggatcca gagcgatagc tttgccgata gcctcaagtg cggcatcatg
    14101 agtaccggcg gaaaaatgcg cttcggacaa cgtgtaaagg taagaagcgt ttttgctatc
    14161 cgaatgctgg acggcggctt gcgcgttttc tacggctttg gcaggaatac gtaaggtttt
    14221 atctttggcc gcgatataaa tccatgccaa gccgttgtac gctaaagcga acttagcgtt
    14281 gatagctatt gcagactgat aggaagtgac ggcttcggca taattttttt ccatcgccag
    14341 tacgtcggca agatgtgtat gagcaaaagc ggatttggca tcgagtttta tggcttcgcg
    14401 aaattgctgt ttagcttctt cgtattggcc ttgtatggta tatacctgac cgagcaggtc
    14461 acgggaaccc gcatgtttag gattgagtgt gatggcgcgt tggtacgcct cttcggcgtt
    14521 tttgtagtcc cctttgtcga aataatcgcg cccgagtccg cggtatccgg aggcaaattt
    14581 gttattgtac tctacgcttt ttttgcgata aaaaacggac gagtcgttat ttttatgagc
    14641 ggggaaaaaa ttggcagaga atgcgtcggc cagcatatcg tacgcatcgt attgactggg
    14701 cttgagtgcg acggctttgc gggctgcttc gatcaactga tcgcgttctt tcagttcgcg
    14761 gtacgtcaga gcgagagccg tccatgctac agcaagattt ttatcaagtt tggcggcttt
    14821 gagaccggca tctaaggcct ttttataact ctcggttttt tcatcagctt taaaattaat
    14881 atcctgtatg cggccgattt tggcataggc ttttgccatg ccggcgtatg ccatggcaaa
    14941 gctgctgtcg gttttgatag cttgttgcaa aagagcaatc gacgctaaat aatcttcgac
    15001 ggtgctgctt tcgtcatact gcaggatgcc tttgatgtag ggttcgtagg cgtccacatt
    15061 gatcgaacct ttaccggtga cagcggtttc atccgccgca cgttttccga aactaacaag
    15121 cgcacgcgcc gcgatttgcc cgtacatcgc gtaaatattg gtcatggaat atttgccttc
    15181 ggcggtgtaa gcgtttttgg tgtccgcttt gccgcttttg acgtctatga aacgcgcctg
    15241 cactttgatc gaaggtgtag cgccgcccgg aatgtcaaaa gaaccgataa tgattttatc
    15301 cacttccagc gaacgggcaa ccgtcaaggc tttctgttcg tccagcatgt ccagaagttt
    15361 aaaaccgttc attttgattt cagcaagcac atcggggcga tccatgatca gaacagattt
    15421 ttcttcgctc agatcggtgt tgagcgattc gcaaaatcca tgaccaagcc attcgttttt
    15481 ggaaagcata gtaccgttgt aaaacggaac tacggctact ttgagttgcg cccaaagcga
    15541 agcgctaatc atcatggcga aaaaaaacaa attcatgcaa gcgcgcatac agtttacctt
    15601 ttgttttaag actgaacctg gaacatagaa ataagtttat tgcacagacg agatcgaagt
    15661 cgtgtatttt ccggacttat ccgccttgcg tgctttttct aaatatactt tcatggtagc
    15721 ttcatcgcct tttttcttca gtgccttcgc atagctgatc agattgggta cgaatttgtc
    15781 attcttttct tcggacgtga taaattcatt gatggcgtca ttgatgcggt tatgctttaa
    15841 gagtcgctcg ccgcgcttcc atgcgtaaac atattcacgg cttttttcct tcgcattcat
    15901 ccgtcccaag gtttcctgat attcaaagtt gtcgggctct atgctcaacg ccgttttcag
    15961 acgattgata gcggagtcgc gaagctccga tttgttaaaa ctggcttcgt aataggcttc
    16021 cgcagccaca gctaaaaact caggattggt attattggtg agctctgcgg ccatcgcacc
    16081 gtattcgata gctttgccga cattgcgaaa acttttgtct ttagccgtca ggtacatcca
    16141 tgaaaggctg gcgtgtgcgt aagcggcatt gggattgagg tcaacggcat aagtaaattt
    16201 gaggaacgct tctttgaaat ttccctcgtc ggccaatacg tccgcaagat gggtataggc
    16261 aaacgtcgag cgcggatcaa gtttgatagc cgtgttaaat tcggttttgg ctttttcaaa
    16321 ttcgccctgt agataataca gccttcctaa acgatcatga gaaccggcat gttgcggatt
    16381 gatcgcgata gctttggtaa agtacgactc agcttgttcc aaatcacctt tggacgcgta
    16441 gtcatcaccg aggccgcgat atcctttggc gaagttcgga ttggcttcaa cggctttttt
    16501 acgaatgaga atggaagaat caatatcagc gttttttgga aaataatctt cggagtaagc
    16561 atcagcccat aggtcataag cttcggcgtt tttgggatca agcgcgaccg ctttctgagc
    16621 tacgcgcatc aagttatccc gttggctgag ttggcggtac accatagcga gcgccgtctg
    16681 cacatcagaa ttttctttag ccattttccc ggctttggag gcgtatttgg cggattcttt
    16741 gtagtgtttg attttcagat catctttgta gttcagcgaa gcgatattgc cgatcatggc
    16801 gtgcgatttg gcaaggcctg cccatgcgat agccagggtc gaatcggcgg ccgcagcttt
    16861 ctcataaatt ccggcggctt ctttgtattc tttctccgaa ccggcggatg aaaatttctc
    16921 attggcctgt tggactaaat cggaagcgga ttgagcggac agggtcacta cgccgcaaac
    16981 gcacaaaacg gtgatgaaaa aatcaaaacg agatttcatg gataaaactc ctcggtgaag
    17041 tgtagaaccg gaaatgcaca agcaaattaa acaggtaagg cggcgttttc aagctaaatt
    17101 acatgtaacg ggggtgctac aaaaagctga atagccggtg cgctgagtgc aacaccggct
    17161 attcgctgaa tcaggaggag aggaatctaa tgagtttgtt cggttgacgg ttgtttatgc
    17221 gattcgcctt tttcgatttt gtgtttttca tgcggttccc agccccacaa agccagcatg
    17281 atgaaaaaac caatcacata accgacagcg acatgccacc cgtctttgag ccaaaacacg
    17341 acggatttag ctttggggaa aatacccgaa atagccacac ccgccgaaga accaaaccaa
    17401 atcatggatc cgccatatcc gacggcataa gccaaaacac cccaatcata tccgccttga
    17461 tccaatgcaa gtttggtcaa cgggatgttg tcgaatacgg aagacacgaa tccaagtata
    17521 aacgctgaaa accatgaagc cggtggcagt tgatccacgg gcatcatgga agcgctgagt
    17581 accaaagaaa ccaggaagac gctgcctttg agtgcgacgg gtatctcatg ccatgcggtt
    17641 tcgcggataa aggcgccaat aaaaattgca gcccagacgc cgagcgcagg aaaatccaga
    17701 agtatattgg tggcaattgc accgaccaat atcagagcta cgactaagat tttaccccag
    17761 tcgatcgaat ggttttctga accttcggcg cgaatgatgg tttgcagttt gttctgttgt
    17821 attgacgcaa cgacggcaaa aaaagcaaat gcaccgatac tgccgtagta cgcatgaagt
    17881 acatcccaag ggctgacgcc gtcaatccac atcatcgtcg tggtcgtgtc gcccaaaaca
    17941 ctgcctgcgc cgccggcatt gcttgccgcg acgatagcgg caagaaaacc gatgtgcacg
    18001 ttaccgcgaa aaaccgtcag tgcgatcgta ccgccgatca tcgccgccgc gatattatcc
    18061 agaaatgagg ataatacaaa tgtgatgcac aataatacca gcggtccggc catgccattg
    18121 ggaaggtgct tcggaaggat ttccggtata tgcgactctt caaaatgttt ggcaagaatg
    18181 gcgaatccta ccagcaaacc gagcaagtta acgacaatcg cccattcgtg atgaatgtgt
    18241 gcgacaacgt caaatgaagt aaagcttaac ttgtaagtca aaatgacggc tagccctgtc
    18301 aatgccactt gtaaaacata atggtgaaac agagcaacac atagcagcgt aaggccaaac
    18361 aaaataaatt cgaccggaat actcaaaaac aaccattgcc cgtttgacga ttgttggaaa
    18421 agcggatcgc gaacaaacaa aagcgccgaa ataaaaagca aaatggaaaa tattccgatc
    18481 atcaccggcc ttgaaaaaaa acggtttact ttggattcct cagacataca ctcttctcct
    18541 gataaaaaga ttcatagatt atgtaccggt tggtcagatc aatccggcta aacgtaaaat
    18601 aaatgcaaga atactcaatc cgattaaagc cataagcgaa acaaataaat aatccttttt
    18661 gggtttggtt tccgttgtga gttgtgtatg tgttttcata tagaactttc gtgacttaat
    18721 gggcacacga ttattggtat gtcaaaaaga tgttgacgta cggtcgcgtg caataggtgg
    18781 ttcttgttga gtttgtcagc gtgtcaaagc aaatatccaa aaagacacga cacctagaac
    18841 taagatacct aaataaatac cgacggcgat ataacctgat ttgggggttg aagcggcgtt
    18901 tgacatagtt tttcctgtgt ggttgcataa atgtttgata cagcacacaa aggctctttt
    18961 cggatgaggt aatatcctcc gattaaaata tgtcgattgg tgtagtcgtt tatggcggcc
    19021 acacgaatgc acacactcta aaagcgattg tttttgtgta ctgctgtttt taggaaaaaa
    19081 ttatgcgatg ggcggagctc tggcgggctg ttctatggaa aatccgttgt acgacgtaaa
    19141 atgcgttgat ttaacaacac gtaagagtgt tgcgcatgcc ggcggtataa cgaaatcaaa
    19201 aagactgacg taagctatcg aacgtgatag tttttcaggc tgtatgtgac gcgccgcaaa
    19261 gaccttgatg gtgaccgatg aagggacggt cgtcggatcg gtctgcatca catgttgggc
    19321 gtagatcaca cactgcggtt tatccgtcgt cgtgatagcg agatatccga tgaagatata
    19381 cgtcaatatc aacgctttag aaaaggctgt atgtaaaaat gttcgcatca tgcgatgatt
    19441 agtctttact ataaaaaata gcgtacttgt aaagtaaatt atctaaaaca gatggaaagt
    19501 caagcgaaat caaaaaagtg gcaaacgatt ttacaacttt ttgacaaatg atcggatccg
    19561 gaatttacag tccgggcggg atgtttttta actttacggc gccgcggtgg cacgtataac
    19621 aagtaacgtt ttctttggcg ttcttaaatg tactttcgtt gagcgtccga accatgcgca
    19681 tcatatcgcg tgaaatattt ttttcctttt tttcatcgct cgcataatca tccttattgt
    19741 ggcaatagtc gcattcgacg cccatggaaa gggtgtataa acgcatggat tctttgattt
    19801 cttcgatggt ggcgtccttc agttcatcaa gcaccttgat gtttttaggc ctgttggtct
    19861 ggcagcttac aaatccgtac cacgacccga agatcagtac cgtcatgaca gaaataaacc
    19921 atctgagttt catgtaacct ccgaaaaaag atttcgtcaa ttataaagga aatcaagccg
    19981 cttcacaaga gttgatttgt ttcttaactt ctagagggta aaatgtatgc ggcgaagcga
    20041 agaaaatctg ttgtgactaa ccgtgattcc gtgtacttta ttcccgcatt tctataattc
    20101 ccattcacca aaacgatgtt ttctatgtta ccagattttg atgcctctca attcatgatt
    20161 gaacctttgg aacgtgcgtc gaccataccg tcgtcgtggt acacggatgc gcgttttcac
    20221 gccgtggatc gtgaatttat tttttctaaa tcatggcagt acgtcggcca tgtttcgcag
    20281 gtggcggaag cgggtcaata cctcacggcg acagtagcgg ataatccggt agtggtttta
    20341 cgtgacacgg aaaaccatct tcgcggtttt tataatgtat gccgtcatcg cggaggaccc
    20401 ctggtcaccg atgcgcgcgg tacgtgcaat aaagtcttgc agtgccaata tcacggatgg
    20461 acttataaaa ccgacggatc gttgcggggc attccgcggt ggaactatgt agatcttttt
    20521 gataaaaagg attacggctt agtaccttta caggtggatg tgtggggtgg actggtcttc
    20581 gtttcgcttg aaaaagaacc tatgccgctc accaaattat ttcaaggtgc acaagagcgc
    20641 atttcgcgca aggcgtatga cgcggacttg gagcggcgat tgttttcgga cctcacgttt
    20701 tataaacgca tcgtgtatcc ggtgcatgcc aattggaaag tgtatgccga taattatctg
    20761 gaaggttatc acttgccatt cgttcatccg gaacttacgt ccatgcttga ctatgcgcag
    20821 tatgtgacgg agacgtatga atattattcg atacagtaca gccctttttc cgaagagggc
    20881 aacgtatata agaccaatga aggcgaagcg ttgtattatt ttgtttggcc taattttatg
    20941 ctcaatattt taccgggacg tttgcaagtc aatcgtatca tacctgacgg tcacgaacgg
    21001 tgtcatgtga tctttgacta tttttacgcg gatctttcga cagaggccaa acgtgcgatg
    21061 gcggaagatg acatcgccta tagtgataaa gtacaaaaag aagattgtga tatttgcgaa
    21121 catgtacaca aaggacttat gtcggacgtg tatgaccgag gccgattttc ggtagagtgt
    21181 gaaaatggcg tgtatcattt ccaaaatcta ctcaaaaaaa actatcaatc ggtttcacaa
    21241 gataatcgtt aaagcgattg agtcgaactg cgaatgacag aaaaaaaaga acttgcacaa
    21301 tccatcgcgt tatgggatgc gatcatgctc aatgcgggca gtatgatcgg ctccggcatt
    21361 tatatcgtac cggcggccgt ggcgggttat cttcagggtt cggtttggat catgacggca
    21421 tgggttgtcg gtggattggt tagtatgttg ggtgctttgt gtttggcgga attgggtgcg
    21481 atgatgccaa aggccggagg aatttatata tacctcagtc gcgctttttc gccgatgtgg
    21541 ggttttcttt acggatgggc caatttcgca gtgattatga ccggctctat tgcagccgta
    21601 gctgtgggtt ttgcgacgta tctcggatat tttatcccgt ttacaccgga tgaaataaaa
    21661 gcggtggcga ttgccagcat catatttctc accgccatca atgttgtcgg aatccttttt
    21721 ggtgtggccg ttcaaaactt catgacgctg ctcaaaattg tttccctcgg aatattgatc
    21781 attattccga tcgtagtacc cggcggatcg gtcgaaaatt tcacgcctct tttttctgac
    21841 ccaaattccg gtcattggat cggcgctttt ggtcttgcca cgattgccgt gttgtgggcg
    21901 tttgacggat ggatcgaaat cacctatgtg gccggtgaag tgcgtgatcc gcaaaaaaat
    21961 gtacctcgcg ctttggtcgt atctaccgcg ctcatcatcg ggctctatgt tttgatcaat
    22021 atggcgtatc tttatgtttt gcccgcggat cagttgcgcg cttcttcgct ggtcgcatcg
    22081 gatatggcta aaattttaat gggacccgaa ggggcggctt ttgtcgcagg atcggtcatg
    22141 atttccatgt ttggtgccaa taacggattt gtcctcagcg gtgcacgtat ttattacgcg
    22201 atggcccgcg agggccgttt ttttgaaaaa atcggcgatg tgcatccgcg ttttggtaca
    22261 ccggcggtat cgattatcgc acagggtgtt tggtcctgcg gattagtatt cacaggtact
    22321 ttcgagcaac tcaccacgta cgtcgtattt gcatcatggt ttttttatgc gatggcgtgc
    22381 atggcggtta tcgtgttgag aaaaaaagat ccgcaaaccg aaagaccata ccgcgcatgg
    22441 ggttatccct ggactccgat cgtgtttatt gcgtttgcgg cttttttgat cggtaatgcg
    22501 gtcatggaag atttgcgttc atcattgatc ggtgtgggaa ttatcataac cggattgccg
    22561 ttgtatgggt attggaatca acatcgaagt cggtaatttt attatctttc aatctatctt
    22621 atccaaaaaa tcatttatgc gttttacact tattgtgttg gctctgcttt tgtggtgctc
    22681 cccgatctat gctcaaaatg aaaatccggc ggatcagcga attgattcgc tgaaggaagc
    22741 cttcgaacga gagccctctt cgcaggctat tatggaggcc ttgggtcgcg cgtatgctga
    22801 aacgcaatcg tatgaagcga ttattgattt gataaagccg gtgatcgaag atacatccac
    22861 aaacaatgtc ctgatcaaac tgtatgtcgg cgcgcgcatc aaacaacttc agacacagtc
    22921 agccgggccc aaacttttgc aacaactttt aggcggcgcg gacaccaaag agaaaaaact
    22981 gattaaccgc gttatgcagc gcgatccgga agatgcggag gcgcaacttt ttttagccga
    23041 gttgcataag ttggccgatg aaccggttta tgcggaaaat atctatcgga ggatgatgaa
    23101 aaaccctcgt gtcatttccg gcccttggtt tgtatcggtt tggacagcgt atagtacgct
    23161 tcttattgat caggagcgtt ttacggaggc caattccatt ctaacccatg ccgcaggtac
    23221 ggaaaaaagc agcgggtggt ttgaacttca gatggctcag gtgaccttgg aaacaggtga
    23281 aacagcacgc ggtacacgat tgttttttca aggtctcgat gcactgcggg atgaatctca
    23341 ggtacatcaa ctttttgaag aggcgtgggc gatcgcggcc cgaaccgaac ttgagcagtg
    23401 gaatacattg aatacgctcg atgcaaaaaa agattttctt cgtcgctttt ggtcaaaacg
    23461 gactcccaat cttgccgact cggctaatgc caaacttgct gaaaattata aacgtttgcg
    23521 tcatgtaaaa cggtattacg cgcgacgcac ctttccgggt tatgatgaac gcggattggt
    23581 ttttatgcgt atgggcaaac ccgatcactt ttataccgat acccgaaccg aatcgtgggt
    23641 ttatgaatcc gccaatgcgc attttgattt tgtagatccg ggcagcggtt attatgaaat
    23701 gcgcccgttg accgatgctt tgagtttgtc gggaagtgcg gctggacagt ttcaggaact
    23761 taaagcgatg atggaagcac gtcgcaactt acatccgttt tacgagcaga cggccgcgcg
    23821 gcttcaggca cgtgaagatg caatatccgt agaacgcagc cgtagcaatg cgtttcgaaa
    23881 tattatcagt gaaactgaaa tacgtttggg gcgcgaaggt acgaataaac cgggtcttca
    23941 aaaatccgaa acgttggaca aaattccttc ggtgtcgctt gtggcatcgt atgcgtgttt
    24001 taagggaaaa aataaatcta ctcgcgttga tttttattat atgattccga tccgtgagtt
    24061 gcagaccttg cgtatagctg attcctcaag tacagcgttt ctatttgcca atgcgcgtat
    24121 tttggacacc aactacagag agattactac gcttcaccgc gaatttaaaa tagccgttgt
    24181 cgatcgggac gcgccgcttt ttgttataga cgagttgcgc acgttattgc aaccgggtac
    24241 gtatattctg ggacttgagc tgattaataa taacaacgag cgcaaagggg tgtatcgatt
    24301 tgatgtggaa gtgcgtgatt ttatcggcgc caacttatcc atcagtgata ttcagccggc
    24361 tgtttcagtg acggttggtt tgctggaaga taaatttatc aaacccgata cacgtatacg
    24421 tgtagtgcct tttgccggcg atcactttga cggtcgtaaa ccgctgtcgg tgtattatga
    24481 aattaacaat ctgacacgtg atctaaaagg cgtgacatcc tacgaaatca gttatacgtt
    24541 ggcaccggct caacgcggaa atattttaac acgggctgta ggtaaacttt ttggtagcag
    24601 taaagcgacg atcacgtcgg tgacccaaaa aaccggaacc ttgccgacag aacgggaata
    24661 cgtgggattt gatatcagcg aattgccgga aggcgaatgc gttttgacga ttaccgtcaa
    24721 agatctgtta tccggtcaga cggcttcgtc cgagcggttt ttttgggtta ataagaaata
    24781 gataactcgc cttcgtgttt accggtcgag gtggagttag tgcaacgatt aaaaagtttt
    24841 agcgcttttt gtgcttccag catcctttga cgtgatcatc taccattccg gaagcttgca
    24901 tgtgcgcata aatgacggtc gaaccgacaa atttaaaacc acgtaatttc agatcagcgg
    24961 acaaggcatc tgattccggc gaagtggcca tgatattttc gcgggagcgt tttggattgc
    25021 gtagcgtttt atacccggta aaagaccaaa tataacggtc aaatgtgccg aattcttttt
    25081 gtacttcgag aaaacatttg gcgttgttga tcgcaccttc gattttgagt cggttacgca
    25141 cgataccggt gtcttgcatc agacggatga cgtccgcttt tgagaagcgt gccacttttt
    25201 taggatcaaa ttgtttgaac gctttgcgaa aattatcgcg tttgcgcagt atcgttatcc
    25261 aactcaaccc ggcctgaaat ccttcgagaa ttaaaaactc aaatattttc tgatcatc
//
LOCUS       DAOQGF010000041        24579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_937_length_24579_cov_6.295955, whole genome shotgun sequence.
ACCESSION   DAOQGF010000041 DAOQGF010000000
VERSION     DAOQGF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_24579_cov_6.295955"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1083)
                     /locus_tag="PK937_07550"
     CDS             complement(31..1083)
                     /locus_tag="PK937_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH32461.1"
                     /translation="MNYIFVLWTTLIWGMFAALFVLQSYFFSIAIGQSFDLWLRLSFE
                     GSQALIWAILTPVVVSFAKRWNLHGASAPASLSVHFVLGFVIGYIHRAASFWLYIALY
                     PPDTPLPSSVAWAKILGSTMNSVLIYWCIVAVGYVVHYVRAHQQERLQAAHLESLFQQ
                     SRLESLRRQIQPHFLFNTLHSISALMDDDVPGARRMITRLSDLLRKTLDQSERSKIPL
                     GEEIRFIADYLAIESVRFQDRLQVRYEVAPEVEKALVPSLILQPLVENAIRHGVAHVA
                     SPSVIIIRALRNEENLNITILNTSVPVNELEKGIGLKNTESRLQLAYGERAALKILTI
                     QDQFSVTIEIPYEDRV"
     gene            1653..2537
                     /locus_tag="PK937_07555"
     CDS             1653..2537
                     /locus_tag="PK937_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH32462.1"
                     /translation="MRLYSISWLIAAVLASLLLTVSGHTQTRTDFGQVIVYGQGPDII
                     LIPGLGCSGEVWKTTVGQIEKTNTCHVVTLAGFAGVPAVSGDFLAPRRDALVAYIRKN
                     CKTKPIVIGHSLGGFLGLMIASEYPDVIGKLIVVDAVPFLPALANPAATVELAKPQAA
                     MMERMLLNQDAVNFAQSQYATIRTMVSDSTQSRTVAEWSIASDRTSLTAAMREMMTTD
                     YRKNISAIRVPTLVLGSWGGYKTFGMTSDMVLGLFQQNYAAQPSVTIHMAPTARHFIM
                     LDEPNWFFSEVLSFLKTR"
     gene            2582..2776
                     /locus_tag="PK937_07560"
     CDS             2582..2776
                     /locus_tag="PK937_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015299891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32463.1"
                     /translation="MFKRLCQNVKHRPGFAVSAGIGIALGASLNDLAMGLAIGMLIGY
                     GMLSCRCMIFKKRTPNPTAQ"
     gene            2831..3868
                     /locus_tag="PK937_07565"
     CDS             2831..3868
                     /locus_tag="PK937_07565"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH32464.1"
                     /translation="MNQHQRMYWLCQLGGWLGYAAINCLFMAGFYHLTVPLVLANVFV
                     SAMGLIGTHAFRSAIRRHDMLSWSLLRLIPIILFKTLLVAAIMECGVFLSSHFVPDAP
                     REFRFAVTLARMFNFSVVALLWSVIYFSIHFFQDRKNSQIKNLKLESALKDAELMTLK
                     SQMNPHFLFNALNSIRALAGSDGARAQEAITKLANILRYALQNAAAPEVTLQDELQTV
                     QDYVALEKIRFEERFNVTWRTAPGLEQIKIPSMLIQMLVENAIKYGIADRTQGGTVII
                     QTERIGSTMEITVINDGHLRQTDASTGIGNANIRQRLALLYGSSAALELTEKNAQVYA
                     KVKIPIREEKP"
     gene            3865..4572
                     /locus_tag="PK937_07570"
     CDS             3865..4572
                     /locus_tag="PK937_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH32465.1"
                     /translation="MNALIVDDERLAREELRRLLSSHPDIKIVGEARHAEEARELIAT
                     LRPDLVFLDIQMPGEDGFALLASLESTPEIIFTTAYDQFALKAFEVNALDYLLKPIDP
                     KRLADALQKISSQESSATPTGPLTDNDQVFVKDGDRCWFVRLKQIRLMESEGNYTRLY
                     FDKEKPLILRSLNQLEVRLDPKVFFRASRQHIINMQWIETIEPWFDGGLNVTLKSGPQ
                     VKISRRQAQKFRELTSL"
     gene            4747..6597
                     /locus_tag="PK937_07575"
     CDS             4747..6597
                     /locus_tag="PK937_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNH32466.1"
                     /translation="MLSSKTLRIIILVFAVTALFCFLPGAAFAGGGEEGGHSLIQSIG
                     ISIIAATVLAFTFHFLKQPLLLSYIAAGVIIGPIGLHLISSKEDIETISEIGLILLLF
                     MIGLEIDMKKLKESGKALIMSGVFQFVLCVALGLAFFPLLGFSIGGGKYDLLYLAACC
                     ALSSTAIVVKLLYAKFELDTLAGRITLGVLVFQDIWAIVILGVQPNLASPDILTILMS
                     FGKGGLLVGIALLISKYILPKLFKSIAKIPELVLVASLGWCFLICSLAGFLGLSIEMG
                     ALIAGVAISTFPYNLDVIAKVISIRDFFVTLFFVALGMKIPNPMDDLGILAIAAVTAV
                     FLIISRFLSVYPILYLLKQGNRISLLPSINLANLSEFSLVIASLGYSAGHIDNKILSI
                     IIFVFVITSIGAPYLIKYSLHIQTALGKFLPKIGLKDVQTVIEKKEEEEEKDIALLGF
                     FRVASSLIKEIQDDKNANNDKAEHEDLFDKIVVVDFNPNVHAKLQALGVKAMYGDISN
                     MDTLHHAGIHGAKLVISTIPDTVLVGTDNLKMIRQIQSLCPHAKIIVTAESAQRAQKM
                     YAEGADYVFLPRVLAADHLKQVIGEALNGDLEKIKKAHIEKLKTVDEIIN"
     gene            6692..7873
                     /locus_tag="PK937_07580"
     CDS             6692..7873
                     /locus_tag="PK937_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNH32467.1"
                     /translation="MQRFWETRFYRRFKRRALEIANNTYFQIFTGILLCVHIGGLLIS
                     FLEDTSANQASTAIRTWYEGIWWAFVTVFTIGYGDYFPITTGGRFLAVGIMVSGVALI
                     SLLTATISSAFVEQRIRKGQGLEKVKLKGHVVICGWNFNVESIIKTLEAELDYPSIVL
                     VNSCDPSPIGEIINAHTNSEISFVAGDFSKDAILDKANIRHAECVIVVADSNELTSNK
                     ADEKTIITVLTVKNMNPKIRVFAHVINPDNAPHLRRAKADDIVISDKYSGFLLAMHVT
                     NPGVPRLFDELLSIEGNEIVRLPIPDTFIGKPFWELTNHYKDKNDCMLIGIAQETRTI
                     AMDDLLSDDNSYLDQFIRAKLQESGKRFAEEERVKIKLNPSKDLVIENNMVAVVLGNK
                     F"
     gene            7895..8401
                     /locus_tag="PK937_07585"
     CDS             7895..8401
                     /locus_tag="PK937_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH32468.1"
                     /translation="MLEVEFLKKFPLFNGLSDAQLGKLKSIMRETDFETHQIVIRDGD
                     HGEEMFVLLDGDVEISKPMMLKVETEMAKGKDKSLIRLSSKFYACFGEMALFEEKSER
                     SATVTTVTHCQMAIITRHDFTKLVETDYELGYIIFRNMAKIISDRLKKANKDILKLTT
                     AFVLAVQQ"
     gene            8430..10022
                     /locus_tag="PK937_07590"
     CDS             8430..10022
                     /locus_tag="PK937_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNH32469.1"
                     /translation="MPAHDLLVTAILLLFIAIIVSIGIRRIRLPYSIGLVVAGFAVGY
                     LRVVPSFSITPELVFYIFLPPLLFDAGFNANLTNLKQNWKIIFTLAVPGTIVSVALVG
                     SIAHYAMDVPWMSALLFGALIVPTDTVSILSVFKELKIPTRLSTIVEGESLFNDGTAI
                     IVFKLILGLILAEQYNVTDINYASFSWHLLLSYTGGLLLGAAVGYGAGWLMRRLKDPL
                     VEIMFTVMLVYGMYIVAEKIEISSIIAVVTASMMIGHYRQKLGMSPTTQIALSSFWNF
                     TAFAINSILFILIGLQLNFNNLLENIGIILIGLLAVNIGRIAFIYPFSNILNIFLGRS
                     ASTKHAVIPIKWQHVMALGNLKGSLSMALVISLPESLLYKNQLMTLTFGIVFLSLVIQ
                     GTTLRPILRRFQLQTIPPEQIEFDKRQGLILSAKAVLASLQSEYTSGMITTSVYNTLK
                     EQYEFTIENAEKSLHRLQEKNPSLADAHLQSAYYQMLILQRTVIQNAQTHNIISDDAA
                     ADLLKNFDQQMAALTWSNQDKS"
     gene            10046..11716
                     /locus_tag="PK937_07595"
     CDS             10046..11716
                     /locus_tag="PK937_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH32470.1"
                     /translation="MHRYILIIILMCLSACGTRANIDMLVVNGKFVTMDDELNNNEIE
                     SIAIRDGRIVDMGKAEVMKKKYVTNRIEDVKGQLVLPGLIDAHLHMLNIGHYLTQLDL
                     VGTESYDQVITLVRDRVANTNKGAWIRGRGWDQNDWSKKDFPEHAALTAVSPDHFIVL
                     ERVDGHAVLVNQNVLTAAKINRETPDPRGGKIMRNAQGEPTGVLIDNAIDLLIPYIPK
                     YTLSEDSLALEAAMSACLKVGLTSVHDAGVDTNHIDVYKELGRRNKLCVRVYAMLNGF
                     DRRLLDMYYKKGPEKNLFNQFLTIASIKLYSDGALGSRGAALLEPYSDSPQSKGLELM
                     MKPRLEIIATDALKWGFQVCTHAIGDRGNRNTLDAYEAAMKAVPDSATYKRFRIEHAQ
                     VVNEADIPRFATLGVIASMQPTHCTSDMYWAEERVGKDRIRGAYAWQKFIKNGVRLAA
                     GSDAPVESHNPLLGVYAAVTRQDAKGWPPGGWYPDQKLSILEALRAFTIDAAYAGFEE
                     TQKGSLTVGKMADIVVLSKDITTADPREILNTHVVWTMVGGKIVYRQP"
     gene            11713..12585
                     /locus_tag="PK937_07600"
     CDS             11713..12585
                     /locus_tag="PK937_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32471.1"
                     /translation="MKIRIRIGGFLLLWYIPRLTTQQIFLMIGVVLSVLINLAAIFFI
                     EIVVNPTKPPQLISDSEKAVLLKLLEEPDMSKVRDLLEQPKANDKVAQKPDALSDRNS
                     TTDVKPPDEIKNFGSVDVTQNQEPRPKTENTTPPDESTDEDRFQNNMNVQPFLKRNTN
                     QKSVYERLTGQEDPSQQGFAGQNYELNTYKWEFAPYMLAWKNKMTSNWYRITSKIFFS
                     QFARVGEILIYVKMNRQGQLVDSKVVDYNCDKSFVAPAYASVVNSFPLDPLPNDFPED
                     MLETTWTISITNQN"
     gene            complement(12599..13648)
                     /locus_tag="PK937_07605"
     CDS             complement(12599..13648)
                     /locus_tag="PK937_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32472.1"
                     /translation="MDSTIWDMQGQSGFVDYQGRKSIFLNGGAAVLKNLELRDGVMDV
                     DVSTPAARGFFGIQFRISEDGLNGEWVYLRQHKSGLADAMQYTPILNTGLNWQLYSGP
                     GFIGAVDIPKNEWFHLRLEVSGAQARLFVKNMDKPVLVMDDLKSGIQKGSIALAVLTG
                     ATYFSNFEVKKTTDAPWVRKHPAMLPNVLKQWEISPSMDALARNVESPLTQAEKKSIV
                     WQSVETEAPGMVTLSRYREAPHLRVSFANDFSKRLEAQPGSKVVYAKTKITSDRDQIK
                     KLYIGYSDEVCVFLNGQILYRGRSAQYFRDPGFLGIMDSENDALYIPLKKGDNELILA
                     VSELGGGWGFICRLE"
     gene            complement(13882..14592)
                     /locus_tag="PK937_07610"
     CDS             complement(13882..14592)
                     /locus_tag="PK937_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32473.1"
                     /translation="MERYTYMIFKTICYILLTSPLMGQSEIWLKLEKGDAMYLDVTSM
                     KWQPLGSKEKIAPRTYVLTKADATLKIYRETNVYDAPAGGYFYIEDMFVNNKRDLVSE
                     ITRIEAEQLPSQPVERANDQKFGLTYGRANETLAENQSVPYLSERMRAIRWFEEHQYA
                     ETALLSLKRLISKFPSLYFEPSNFDRLCSLYMKLGLYGFLFDETNRFMTLKRDDEFGQ
                     MVIRWNETAKKKLTTSGQ"
     gene            complement(14564..16534)
                     /locus_tag="PK937_07615"
     CDS             complement(14564..16534)
                     /locus_tag="PK937_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32474.1"
                     /translation="MTRWGLLMLVFVSASLFAQNDADAYRPMRYDARLTFVSEYTARQ
                     FASKEYVASRIHYMHNHGLYGFSVLVNMNQQLPFTIMPGTTTLVLPDSVKLVDRSQIT
                     NHAHTYVVDLPAALELRRRFARFEIAFGSQILVEKEEIVSTLVKELTSKVYYVDYEFA
                     SHRRSSLLGYGAMTSYWGPFAVTTGILPWRMIRMGNDSMHYALKNKLSGFLDVQYRYR
                     SHSMTTRVGRDAWGGNVQLKYDALQVNNEYCRSLRPSDYQVWKLNLSYDLSGGFTLLA
                     GYESTWYLAENFRSQNFRQWLNRHGDISSGKLTNQLPHKTVQIGLRYDLSGSRAADYV
                     KVVNMKIFQTKVYTAKRAYYAYNPVGQMDVHNASDEHLNVVIHAEAGAWGEIFRSSSI
                     DLAPHEIKSVPLFFYIPDQSVEQSGMVAQVKITGEVRGKHIALSSFPVTLYDIHSWDG
                     DTWGLRYFIAPDDPEIQSFAKSNLLTIGTDSAYSELYNKFHTLKKFVTAVGKNLRYVS
                     DPTTTFVNERVQYPSETLRQKSGDCEDLVAFMATNLMAIGHACAVVDIRPTLTENINI
                     PTAKPGSIGHVFLLVDTSIPVNAVDQLGLSEFEYVARPDKTGRVTIWLPLETTVVNGG
                     FEHAHREGVRQYYDEVIQKNGVVHGTVHVYDF"
     gene            complement(16527..18698)
                     /locus_tag="PK937_07620"
     CDS             complement(16527..18698)
                     /locus_tag="PK937_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH32475.1"
                     /translation="MKKIVVCLSLILFCNSLLTAQERPVIRIAIGKHDVEHLGGWPLA
                     RRWYAIAAQHAKQNGAQAIYFDMAFPSADALHPESDEVFYHECVRDDKIFLMTERVQE
                     NNTILGGQSFPAGRTFLPFAAHWQIENAYLIYTPGDHSLVSALTEVPEETVILHWPSD
                     TLLPDMHLSAFLSSNSDLSNRSVVFYFDYPGVTSYVASPQGSVNPVSLIQIWAAEEIK
                     QGRMWKQWPLWLLVVLFAGTAVVSFLSLRDTRWIWIGAPALIWCAFAAGLYFLRIYLP
                     IGIGWTLVPILVSTFMLGIKRRTVTVTKEVASAASDDLTDLRYRLNFYEKISDQIRPL
                     DLSECREAQGIYYHKDSPVAELLQKADQVAQSNVPVLITGESGTGKERIAEFIHRRSQ
                     RASGPFVAVNCGAFNDNLIEAELFGYEKGAFTGAVQAKPGRFEMADNGTLFLDEIAET
                     SPAFQVKLLRVLQEGRFERVGGVVSKSVSVRVIAATHRDISVLIRDKQFREDLYYRIA
                     GFVLPLKPLRERTVDIDFLFRQFVYEIAPELKYSDHLVQWLCTLAWPGNVRELKAATQ
                     RSVINARMRHRNYILPADFEIRGATFSDSEQSLAERVLHALRDHQFKHRAMSAVADDL
                     GLHRITVTEYFRGWVIKAMIRCEQEPQAMFEYLRGTSDISEPKALKQRIDEYQNNISE
                     RIRTGVKKNESPEEVIRLRFRNTPKFFYDDLIQLIKEYRHD"
     gene            complement(18745..19650)
                     /locus_tag="PK937_07625"
     CDS             complement(18745..19650)
                     /locus_tag="PK937_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32476.1"
                     /translation="MSKSVALIVTIALLAIGCDKDGAIVKSDVGSDQTLIISHDPNKG
                     GTIGAVLPPPDSMLYVFIGNTDTSSSSTSKYFMFLEHDLNTNINGRTLKKYLDYGTLN
                     VGGFAAQRYHTYYVVNVPDSGGIPAHKDTVDATRYDGFPLTLPLDPVNNKLTISGSNS
                     THINNFSTDISVPTQLKFINLTFNQTIGDNEDLHLKLNRVSPAGFVGFVPQNIKFGDS
                     TSVGYIYSFFDYTVPTDEVVISKQHLEQLISKWNQDEVIYHLFVFEENVVGSINTIDK
                     TNGQSYGLPVEIDYTHNVLVKLKKQ"
     gene            complement(19953..21650)
                     /locus_tag="PK937_07630"
     CDS             complement(19953..21650)
                     /locus_tag="PK937_07630"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNH32477.1"
                     /translation="MKSDIEIAQESPITPITEIAKKIGLNASDIELYGEHKAKIKLEV
                     LDRLKDKKIGKFIFVTAINPTPLGEGKTVVNIGLSQALAKIGKNVISTLRQPSMGPVF
                     GVKGGATGGGYSQVQPMEDINMHFTGDFHAITTAHNLLAAIIDNHLHKGNPLNLDINN
                     IYWRRVMDMNDRALRDIIVGLGGSNNGIPRETGFDITAASEIMAILSLAEDLKDLRKR
                     LETILVGTSRDKKGVYTRQLNVVGALMVLLKDAIKPNLVQTLEGVPVIMHAGPFANIA
                     TGNNSVIADKIALRLADYVVTECGFGADCGAEKLINIKCRQSGLRPSAAVVVATVKAL
                     KMHGGGFEAVPGKKIDKALLEKENVEAVIKGCENLTKHIENIRSFGVPVVVAINRFTS
                     DTENEIQAIRDIAVKTGARAVIPIDVWGKGGLGGTALAEEVVKACDEKSEVKFTYDVQ
                     GTIENKMATVVKTIYGGAGISLSSKAKSKIKMLKEEGLDKLPICIAKTHLSLSHDPTL
                     KGRPKDFTVPIDDIKASAGAGFIYAIAGQMMTMPGLPSVPSSEAIDMDDNGVIRGIF"
     gene            complement(21786..22556)
                     /locus_tag="PK937_07635"
     CDS             complement(21786..22556)
                     /locus_tag="PK937_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH32478.1"
                     /translation="MKLHFVEYGSPKTLVILHGLLGSERNWYSVAKKLSDHYRLIIPD
                     LRNHGVSPHDPEHSIVAMRKDIEALVDRLHLHEFYLLGHSMGGHVAMNYAFAHPERVK
                     ALIVEDIAPRSYGTGLIEILTAMNAVDLNLYKEKKQVEVALAEGIKNPAVRSFVITNL
                     VRDHDRMYWRVNLPALTEFAANEIVRFKASENDRFHGPTLFVGGEKSMYNLRDDAELI
                     AKHFPSSALEMIPDAGHWIHHEKADVFCQIVMDFLNRH"
     gene            22698..23546
                     /locus_tag="PK937_07640"
     CDS             22698..23546
                     /locus_tag="PK937_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNH32479.1"
                     /translation="MDITYLTFVLGAVVTLLILARLVNQFLMRERLTDALTEKDRLAT
                     GIALSGYLFGVMMIIIDVLSGEGHNDWLKDFLLVGVYGLAGIVFLVFVSVMELRLILS
                     AKALDAVREGNVAAAITVAASYIATSQIIGAVVSGDNTGGNWLTAVIFFIIGQATLLI
                     MTYVFRWLTAYNDAEAIMQGNAAAALSYAGVMIGIAIIVGTALRGDFIDYPTSLIDYA
                     WSLVIVVALYPIRQFLVQGVLLGCGFSFYGGKLDEEISKDRNIGAGCIEATTYIASAL
                     LATALL"
     gene            23560..>24579
                     /locus_tag="PK937_07645"
     CDS             23560..>24579
                     /locus_tag="PK937_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNH32480.1"
                     /translation="MRTSEVKYTDPSVPRRDPYFDFAKKITDSHILTDPWIDGDPRFS
                     DEPVILGAGTYALMQKAAEHIGALYDELVRIILREPHWLDDYFHLTPHQKMLWFASGG
                     RWHGMARLDMFLCTDGRLQVCEMNSDTPSGEAEAVLINQIIRSRFLRFTDPNAGLETQ
                     FIRMVLRLWGKRTWPRLSPTVGIVYPTEMPEDLSMIRLYQRWLESRGAHIILGSPYNL
                     GSTAQRLTLLQKPVDVLIRHYKTDWWCERLPVWKDQEPFPDALPLVKPLQHLMRAWLA
                     GQITMINPPGAVLTQNKLTMAFFWEHLRKFSPTARNTIRMWIPETRRLITKESKKLNK
                     NEWVLK"
BASE COUNT         6598 a         5920 c         5368 g         6693 t
ORIGIN      
        1 tcatctgcga cgaccgccat taattcatga tcaaactctg tcttcatagg gaatttctat
       61 cgttacgcta aattgatcct gtatagtcag aattttcaac gccgcccgct cgccgtaagc
      121 caactgcagg cgagattccg tatttttcag tccaatcccc ttttccaatt cgttgaccgg
      181 taccgatgtg tttaagatcg ttatattaag attttcctca ttccgaagag cgcgaataat
      241 gatcactgac ggtgatgcca catgagcaac gccatgacga atggcattct caaccaaagg
      301 ttggagaatc agagaaggga ccaatgcttt ttcaacttcc ggagccacct catagcggac
      361 ttgcagacga tcctgaaacc ggacggattc gatcgccaga taatccgcga taaatcgtat
      421 ctcttcgccg agcggtattt ttgaacgttc cgattgatcc agcgttttgc gcaacagatc
      481 actcaaccgg gtgatcattc gccgcgcgcc gggaacatcg tcatccatca gagccgaaat
      541 cgaatgcaac gtattaaaca aaaaatgagg ttgtatctga cggcgcaacg attccaaacg
      601 cgattgctga aaaagacttt ccagatgcgc tgcttgcaat cgttcttgct gatgcgcacg
      661 cacgtaatgc acgacgtatc ccacggcgac gatacaccag tagatcagaa cactattcat
      721 agtactgccg agaattttag cccaggcaac cgacgacggc aggggcgtat ccggcggata
      781 cagtgcgata tagagccaaa acgacgcagc gcggtgtata taaccgataa caaatccaag
      841 tacaaaatgt acggacaatg aggctggggc ggacgcacca tgaagattcc atcgcttagc
      901 gaaactaaca actaccggtg tcaaaatcgc ccatatcaat gcctgtgaac cttcaaacga
      961 taggcggagc cacaagtcaa acgactggcc gatcgcaatg ctaaaaaaat aggactggag
     1021 aacaaaaagt gcggcgaaca tgccccagat caacgtagtc cacaaaacga atatgtagtt
     1081 catttttttc attctaaaag gcatatatgt tcaaatatga tacgtttgat tttacgatac
     1141 gttccttaga ttgtaaaaaa aagtattggg aaaaccaaat ttgccaatcc aaggctgcat
     1201 ttggtcaaaa tcgggtttta cctgcatgaa tacctcggaa aagctttcat tcgtaactcc
     1261 cataaactgt acatcaatct gtaaatttca gtaggagatt gatcatgaat tgccccctct
     1321 cttaatgacg aactaactta ttcgagcatg atcttataca aaaagttatt taagtaccct
     1381 aaatttcagt tagcattaca gctttttttc tcaaaagaag taaatccgtg caatcttaat
     1441 gacaacgcaa cctagcaaaa agcctaaatg ataattatag tgactttgca catacggata
     1501 aaacattttt ccgtttatgt agagtcggtt aacgtttacc ctttatctcc gaccgctcat
     1561 ccccttgatc cgccgttcat cccaattggc taaccccctc tcgttttttc gtatagattt
     1621 ctcttgttcg ttaataaaac aaggagaaat gtatgcgttt gtattcgatc tcatggctta
     1681 tcgcagcggt gctggcctcg ttacttttga ccgtttcggg gcatacgcaa acccggacgg
     1741 actttggtca agttattgtt tacggacaag gtcctgacat catcttgata ccgggactgg
     1801 gttgttccgg tgaagtttgg aaaacaacgg tcggtcaaat tgaaaaaacc aatacatgcc
     1861 atgtcgtaac attagccggt tttgccggtg tgccggctgt gtcgggcgat tttctcgctc
     1921 cgcgtcgtga tgcgctcgtg gcttacattc gaaaaaactg caaaaccaaa cccatcgtga
     1981 tcggtcacag cctcggcgga tttttaggct tgatgatcgc atcagaatac ccggatgtga
     2041 tcggaaagct gatcgtcgtg gatgccgtac ctttcctccc tgcactggcc aatccggcag
     2101 caacggtaga attggccaaa ccacaagccg cgatgatgga gcgtatgctg ctcaatcagg
     2161 atgccgtcaa tttcgcgcaa tcacaatatg ccacgatccg cacgatggtc agtgattcga
     2221 cgcagtcgcg caccgtcgcc gagtggagca tcgcatcgga ccgcacgagc ttgactgccg
     2281 ctatgcgtga aatgatgacg accgattacc gtaaaaacat cagcgcgatc cgcgttccta
     2341 cgctggtgct cggttcatgg ggcggttata agactttcgg tatgacgtcg gatatggtac
     2401 tgggtttatt tcaacaaaac tatgccgctc agccttcggt taccattcac atggcaccga
     2461 cagcacggca cttcatcatg ctggatgaac caaactggtt tttcagcgaa gtgctgtcgt
     2521 ttttgaaaac acgttaacac tatactaaaa ccactctttg aattatttga aaggagtccg
     2581 tatgttcaag cgcctttgtc aaaacgtaaa acaccgaccc ggctttgccg tatccgcagg
     2641 catcggtatc gctttgggtg ccagtctcaa tgatctggct atgggattag ccatcggtat
     2701 gctgatcgga tatggtatgc tttcatgccg ctgtatgatt ttcaaaaaac gtactcccaa
     2761 tcctacggcg caatgatgat gagtcgagta aagaatatag cttacatttt atagacaaag
     2821 aatttgtggt atgaatcaac accagcgtat gtattggtta tgtcagctcg gcggctggct
     2881 gggttatgcc gcgatcaact gcctttttat ggcgggattt tatcatctca cggtaccgct
     2941 tgttttggct aatgtgttcg tcagcgcgat gggcctgatc ggtacccacg cttttcgaag
     3001 cgctatccgt cggcatgaca tgctgagctg gtctctttta cggcttatac cgattatttt
     3061 attcaaaact ctgctggtcg ccgcgatcat ggaatgcggt gtttttttga gcagccactt
     3121 cgttcccgat gcacctcgtg aatttcgatt cgccgtcaca ttagcgcgca tgttcaactt
     3181 cagcgtcgtg gcgttacttt ggtctgtgat ttactttagc attcattttt ttcaggatcg
     3241 taaaaattca caaatcaaaa atctgaaact tgaatccgct cttaaagatg cggaactgat
     3301 gacgctcaag tcgcagatga atccgcattt tctttttaat gcgcttaaca gcattcgcgc
     3361 gttagccggc agcgacggtg cacgtgcgca agaagcgatt accaagttag ccaatatcct
     3421 tcgatacgcg ttgcaaaatg ccgcagcgcc tgaagtcacg ctgcaggacg agctgcagac
     3481 cgtacaagac tatgtcgcgc ttgaaaaaat acgctttgaa gagcgtttta atgtgacgtg
     3541 gcgaaccgca ccgggattag aacaaataaa aataccttcc atgttgatac aaatgctggt
     3601 agagaatgcc atcaaatatg gcatcgccga ccgcacgcag ggcggtaccg tcatcattca
     3661 aaccgaacgc atcggctcta cgatggagat cacggtgatc aatgacgggc atctcagaca
     3721 aacagacgct tccacgggta tcggtaatgc taatatccgt caacggctcg ccttactgta
     3781 cggatccagt gccgcactgg agcttaccga aaaaaatgct caggtttatg ccaaagtcaa
     3841 aatcccgata cgcgaggaaa agccatgaat gctctgatcg tagatgatga aaggttggcc
     3901 cgtgaggaat tgcgtcgttt attatcatcg catccggata ttaaaatcgt aggcgaggca
     3961 cgtcatgccg aagaagcgcg tgaattgatt gcaacacttc gacctgattt ggttttttta
     4021 gatatccaaa tgcccggtga agacggcttt gcccttctcg cttcgctgga gtccacaccg
     4081 gagattattt ttaccaccgc ttatgatcaa tttgccctca aagcatttga agtgaatgcg
     4141 ctcgactacc tgctcaaacc gattgatcct aaacgcctcg ccgatgcatt gcaaaaaatt
     4201 tcgtcgcagg agagctccgc tacaccaacc ggaccgctca cggacaacga tcaggttttc
     4261 gtcaaagacg gcgaccgttg ctggtttgta cgtctcaaac aaattcgact gatggaatcc
     4321 gaaggcaact atacgcgttt atatttcgac aaagagaaac ctttgatttt gcgttccctc
     4381 aaccaacttg aagtgcgcct cgatcccaaa gtatttttcc gtgcaagccg tcagcatatc
     4441 atcaacatgc agtggatcga aacgatcgaa ccgtggtttg acggcggtct caacgtaact
     4501 ttaaaatccg gcccacaggt caaaatatcc cgccgccaag ctcaaaaatt ccgcgaactg
     4561 acaagccttt gaagaaacga gtgaatttag aatttagaat tcatcattct taactcatca
     4621 ttcttaatta tcctcatttt ccctccgatt ttatttgcat gaaatagcat tttactttat
     4681 gtttttttgc taaccgtttg gttcgactat aaattcattt tttcatatta atgcgagtac
     4741 ccaattatgc tgagttccaa aactttacgc atcattatcc ttgtgtttgc cgttacggct
     4801 cttttctgtt ttctccccgg tgccgctttt gcaggcggcg gtgaagaagg cggtcacagc
     4861 cttatacaga gtatcggtat aagcattatt gcggcgacgg ttttagcttt tactttccat
     4921 tttttgaagc aaccgttgct tttgtcatat attgccgccg gtgtgatcat cggtcctatc
     4981 ggattgcatt tgatcagcag taaagaagat atcgagacca tttcagaaat tggattgata
     5041 cttttgctct tcatgatcgg cctcgaaatt gacatgaaaa agctcaaaga atccggaaaa
     5101 gctctcatca tgtcgggcgt atttcaattt gtgctttgcg ttgcgctcgg cctggctttt
     5161 ttccctttgt taggatttag tatcggcggt ggtaagtacg acctgcttta tctcgccgca
     5221 tgctgcgcgc tgtccagtac ggcgattgta gtaaagcttt tatatgctaa gtttgaactg
     5281 gacacactcg ccggacgcat tacgcttggt gtactcgtat ttcaggatat ttgggcgatc
     5341 gtaattctgg gtgtacagcc caatctcgcc agcccggaca ttttaaccat cctgatgtct
     5401 tttggcaaag gcggcttgct cgtaggtatt gctcttttga tcagcaaata catccttccg
     5461 aaacttttca aatccatcgc taaaattccg gagcttgtac tcgtggcttc gctcggatgg
     5521 tgttttttga tttgctcatt ggccggattt ctcggcctct cgatcgaaat gggtgccctc
     5581 attgccggcg tcgctatttc gacgttcccg tacaatttgg acgtgattgc caaagtcatc
     5641 agtatccgcg atttcttcgt gacattattt ttcgtcgcgc tcggtatgaa aataccgaat
     5701 ccgatggatg atctcggtat tcttgcgatt gcggccgtaa cggcggtatt tcttatcatc
     5761 tcacgcttct tatccgttta tccgatattg tacttgttaa agcaaggtaa tcgtatcagc
     5821 cttttaccgt ccatcaattt ggccaatttg tcggaatttt cgctcgtcat cgcttcgctc
     5881 ggatacagcg caggtcatat tgacaataaa attttatcca tcatcatttt tgtttttgtg
     5941 atcacatcca tcggggcgcc gtatctgatc aagtacagcc ttcacattca gacggcgttg
     6001 ggtaagtttc tccccaagat cggactcaaa gacgttcaga ccgtgatcga gaaaaaagaa
     6061 gaggaagaag aaaaggatat cgctttgctg ggctttttcc gtgtagccag ctcattgatc
     6121 aaagaaatac aggacgacaa aaacgccaat aatgataaag ccgaacacga agacctgttc
     6181 gataaaatcg tcgtggtgga ttttaatccc aatgtccatg cgaaactcca agcgctcggc
     6241 gtcaaagcta tgtacggcga tatttccaat atggatacgc tgcatcacgc gggtattcac
     6301 ggcgccaaat tggttatttc cactattccc gatacggtcc tagtcggaac ggacaatctt
     6361 aaaatgatcc gtcagataca aagtttgtgc cctcacgcca aaatcatcgt gaccgctgaa
     6421 agcgcacaac gcgcacagaa gatgtatgcc gaaggtgcgg attatgtatt tttaccccgc
     6481 gttttggccg ccgaccacct caaacaagtc atcggcgaag ccctcaacgg cgatctggag
     6541 aaaattaaaa aagcccatat cgaaaaatta aaaacggtgg acgaaatcat aaattaggtt
     6601 tatattgatc gcgggaatga atcaggagca ttcccgcttc atcactataa atcctattga
     6661 tcctcgtatc agagaggttt tcgtacgacg tatgcaacga ttttgggaaa cgcgtttcta
     6721 ccgccgtttc aagcggcgcg ctcttgaaat agccaacaat acctactttc agatttttac
     6781 cggcattttg ctctgcgtac atatcggcgg actgctgatc agttttttag aagatacttc
     6841 ggcgaatcag gcatcgacgg ctatccgaac ctggtatgaa ggtatttggt gggcgtttgt
     6901 taccgttttt accatcggtt acggagatta ttttccgata acgaccggcg gacgtttttt
     6961 ggccgtcgga attatggtaa gcggcgttgc actgatttca ctcttaactg ctacgatttc
     7021 ctccgcgttt gtcgaacaac gtatccgaaa gggccaaggt ttggaaaaag taaaactcaa
     7081 aggccacgtc gtgatttgtg gctggaattt taacgtcgag tccatcatca aaacacttga
     7141 agccgaactc gactatcctt cgatcgtatt ggtcaattcc tgcgatcctt caccgatcgg
     7201 tgaaatcatc aatgcgcata cgaattcgga aatcagtttc gtcgccggcg atttttcgaa
     7261 ggacgccata ttggacaagg ccaacatccg gcacgccgaa tgcgtgatcg tcgtagccga
     7321 ctccaacgaa ctcaccagca ataaggccga cgaaaaaacg atcatcaccg ttcttacggt
     7381 caaaaacatg aaccccaaaa ttcgcgtttt tgcccatgtg atcaatcccg acaacgcccc
     7441 gcatctccgg cgcgccaaag cggatgatat cgtcatctcc gacaaataca gcggcttttt
     7501 actcgccatg catgttacga atcccggtgt gccacggctt ttcgatgaac ttctttccat
     7561 cgaaggcaac gagatcgtgc gacttcctat cccggataca tttatcggta aacccttttg
     7621 ggaactgacc aaccactaca aagataaaaa cgattgcatg ttgatcggga tcgctcagga
     7681 aacacgaacg atcgctatgg acgatctctt atcggatgat aattcgtatt tggatcaatt
     7741 tattcgcgcc aaacttcagg agtccggcaa acgttttgcc gaagaagagc gcgtcaaaat
     7801 aaaactcaat ccctcgaaag accttgtgat cgaaaacaat atggtggccg tggtattggg
     7861 taataaattt taatcgtcaa aaaccggatg atttatgctt gaagtagaat tcttaaaaaa
     7921 atttccgctt ttcaacggtc tcagcgatgc ccagctggga aaacttaaat ccatcatgag
     7981 agaaacggat ttcgagactc atcaaattgt aattcgcgac ggggatcacg gtgaagaaat
     8041 gtttgtactt ttggacggtg atgtcgaaat ttccaaaccc atgatgctta aagtggaaac
     8101 cgagatggcc aagggcaaag ataaatcgct gattcgtctc agttccaagt tttatgcctg
     8161 cttcggcgaa atggctttgt tcgaagaaaa gagcgaacgc tccgctacgg taaccaccgt
     8221 gacccattgc caaatggcta tcatcacgcg gcatgatttc acgaaactcg ttgaaacgga
     8281 ttacgaactc ggctatatta tttttaggaa tatggccaag attatcagcg accgtttgaa
     8341 gaaagcgaac aaggatatcc tgaaactaac cacggcgttc gttttggccg tacaacaatg
     8401 aatggtatta tttttccgac ggagtcccga tgcctgctca tgaccttttg gtcaccgcga
     8461 tcctgttact attcatcgcg attatcgttt cgatcggtat tcgccgtatc cgtttgccct
     8521 acagcatcgg cttagtcgtt gcgggttttg ccgtcggtta tctccgcgtt gttccttcgt
     8581 tttccatcac ccccgaattg gttttttaca ttttccttcc gccccttctt tttgatgccg
     8641 gttttaatgc aaacctgacc aatcttaagc agaactggaa aatcatattt acgctggccg
     8701 tacccggcac gatcgtgtcg gtcgcgttgg tcggttcgat cgcacattat gcaatggatg
     8761 ttccctggat gtcggcctta ctttttggcg ctctgatcgt tccgacagat accgtttcga
     8821 ttctatccgt ttttaaagaa ctcaaaattc ccacacgtct ctccaccatc gtcgaaggcg
     8881 agagtttgtt caatgacgga acggccatca tcgtattcaa actcattctc gggcttatcc
     8941 ttgccgagca gtacaatgtc acagatatca attatgcttc gttttcgtgg cacttgcttt
     9001 tatcttatac gggcgggctt ttgctgggtg ccgctgtagg ttacggcgcc ggttggctta
     9061 tgcgccgtct caaagatccg cttgtcgaaa tcatgttcac ggtaatgctg gtttacggta
     9121 tgtacatcgt tgccgaaaaa atcgaaatct ccagcattat cgcggtggtg acggcttcta
     9181 tgatgatcgg ccattaccgg caaaaactcg gtatgtcacc gacgacacag atcgcacttt
     9241 catcgttttg gaattttact gcatttgcaa tcaattcgat attatttatt ctgatcgggc
     9301 tacagcttaa ctttaataat ctgctcgaaa atatcggcat cattctgatc gggcttttgg
     9361 ctgtaaatat cggacgtatc gctttcattt atcccttttc aaacattctc aatatttttc
     9421 ttggtcgaag tgcctcaacc aaacacgccg ttataccgat caaatggcaa cacgtcatgg
     9481 cgctcggtaa cctcaaagga tcgctctcga tggcactcgt cattagtttg ccggaatcgc
     9541 tgttgtataa aaaccaacta atgaccctga catttgggat cgtttttctt tcgctcgtta
     9601 tccaaggcac aactttgcgt ccgattttac gtagatttca attgcaaaca ataccacccg
     9661 agcagatcga atttgataaa cggcaaggct tgattctttc ggcgaaggcc gttttggcct
     9721 cattacaatc ggaatatacg agcggcatga ttaccacttc ggtttataac acattaaaag
     9781 agcaatacga atttaccatc gaaaatgcgg aaaaaagcct ccatcgtttg caggaaaaaa
     9841 atccgtcttt agcggatgct cacttgcagt ctgcttatta tcagatgttg atccttcagc
     9901 gcaccgtcat acaaaacgca cagacgcaca atatcatcag cgatgatgcg gcggcggatt
     9961 tgttgaaaaa ttttgaccaa cagatggccg cgctcacgtg gagcaatcag gataaaagtt
    10021 aattttatat acgaaagaac cacccatgca ccgttatata ctgatcatca ttttgatgtg
    10081 cctcagcgca tgcggcacac gcgccaatat tgacatgctt gtcgtcaacg ggaaatttgt
    10141 cacgatggac gacgaactaa ataataacga aatcgaatcc atcgccattc gcgatggccg
    10201 gatcgttgat atgggcaagg ccgaagtgat gaaaaaaaaa tatgtgacca atcgtatcga
    10261 agacgtcaaa ggtcaacttg tgcttcccgg attaatcgac gcgcatctgc atatgctcaa
    10321 tatcggtcat taccttacgc aacttgatct tgtaggcacg gaatcgtacg atcaggtcat
    10381 tacgctggtt cgcgaccgtg tggcgaatac aaacaaaggc gcatggattc gcggccgggg
    10441 ttgggatcaa aacgattggt ctaaaaaaga ttttcccgaa catgccgcac tgaccgctgt
    10501 ttcgccggat cacttcatcg ttcttgagcg cgttgacggg catgcggtat tggttaatca
    10561 aaatgttttg acagccgcca aaattaaccg tgaaacgccc gacccgcggg gcggtaagat
    10621 aatgcgcaat gcgcagggcg aacccaccgg cgtactcata gataatgcga tagacttgct
    10681 gataccctat attcccaaat atacactttc cgaagacagt ctcgcgttgg aagccgcgat
    10741 gagcgcgtgc ctcaaagtcg gtctcacatc cgtacatgac gccggcgttg ataccaatca
    10801 tattgacgtg tataaagaat tgggacgtcg caataaactt tgcgtgcggg tttatgccat
    10861 gctcaatggt tttgatcgcc ggcttttgga tatgtattac aaaaaaggcc ccgagaaaaa
    10921 tcttttcaac caattcctta caattgcgtc tatcaagctg tattccgacg gtgcactcgg
    10981 ttcacgcggc gcggcgttgc tggaaccgta ttctgacagt ccgcaatcca aaggccttga
    11041 attgatgatg aaaccccgcc ttgaaatcat cgccaccgat gcgctaaagt ggggctttca
    11101 ggtttgtacc catgcgatcg gtgatcgcgg caaccgcaat acgctcgatg cttacgaagc
    11161 cgccatgaaa gcggtgcccg acagcgcaac atataaacgc tttcgtatcg aacatgccca
    11221 agtcgttaat gaagcggaca tcccgcgttt tgcaaccctc ggcgtgattg cttcgatgca
    11281 acccacacac tgtacatcgg atatgtattg ggcggaagag cgtgtcggca aggatcgtat
    11341 tcggggtgcc tatgcctggc agaaatttat caaaaacggt gtgcgtttgg ccgccggttc
    11401 cgatgcaccg gtcgaatcgc ataatccatt gctcggcgtc tatgcggcag ttacgcgtca
    11461 ggatgccaaa ggatggccac cgggcggctg gtatcccgat caaaaattat ccatacttga
    11521 agccttacgc gcttttacga tagacgccgc ttacgcgggt tttgaagaaa ctcaaaaagg
    11581 aagcctcact gtgggaaaaa tggcggacat tgtcgtttta tccaaagaca ttacaactgc
    11641 ggatccgcgt gaaattctca acacccatgt cgtttggacg atggtcggcg gcaagatcgt
    11701 atatcgtcaa ccatgaaaat tcgcattcgg atcggaggtt ttctccttct ttggtacata
    11761 ccgcgcctga cgacgcagca gatttttttg atgatcggcg tcgtgttatc cgttttgata
    11821 aatcttgcgg caattttttt catcgaaatc gtagtcaatc ctaccaaacc accgcaactt
    11881 atcagcgata gcgaaaaagc cgttttacta aaattacttg aagaaccgga tatgagtaag
    11941 gtgcgtgatt tgcttgagca accgaaagcc aacgataagg tcgcgcaaaa accggatgct
    12001 ttatccgatc gcaattctac cacagacgtc aaaccaccgg acgaaatcaa aaatttcggg
    12061 tcggttgatg tgacacaaaa ccaagaaccg cgccctaaaa cagaaaatac aactccgccg
    12121 gatgaatcca ccgatgaaga tcgttttcag aataatatga acgtacagcc ttttttgaag
    12181 cgcaatacca atcagaagtc cgtttatgag cgattaaccg gtcaggaaga tccttcgcaa
    12241 caaggatttg ccggacaaaa ttacgaactc aatacgtata agtgggaatt tgctccgtat
    12301 atgctcgcat ggaaaaataa aatgacttct aattggtatc gcatcacatc caaaatattt
    12361 ttcagtcagt ttgcacgtgt cggcgaaata ctcatctacg tcaaaatgaa caggcaaggc
    12421 caattggttg actccaaggt cgtggattat aattgcgata aatcctttgt tgcgcccgcg
    12481 tatgcgtctg tcgtaaatag ttttccgctt gatccattac cgaatgattt cccggaagac
    12541 atgctagaaa cgacatggac catttcgatt accaatcaga attaatcccg ctctcgtatt
    12601 attccaatcg gcaaataaat ccccatccgc cgcctaattc actgacagcc aggatcaatt
    12661 cgttatcccc ttttttcaaa ggtatataaa gcgcatcatt ttcggaatcc atgattccca
    12721 aaaaaccagg atcgcgaaaa tactgtgcac tgcgcccgcg atagagtatt tgcccgttta
    12781 ggaatacgca cacttcatcg ctgtatccga tatacagttt tttgatttga tcgcgatccg
    12841 acgtgatttt tgttttagca taaactactt tagaaccggg ttgcgcttcc aatcgtttag
    12901 aaaaatcatt ggcaaaagaa acccgcagat ggggtgcttc ccgataacga gaaagtgtca
    12961 ccatgcccgg cgcctccgtt tctaccgatt gccatactat tgattttttc tcagcttgag
    13021 tcagaggtga ttcgacgtta cgtgccagcg catccatcga cggtgaaatt tcccattgct
    13081 tcaatacatt aggcagcata gccggatgtt ttctcaccca cggagcatcc gtagtttttt
    13141 taacttcgaa attggaaaaa tatgtcgcgc cggttaaaac cgctaaagcg atagagccct
    13201 tctgaattcc gctttttaga tcgtccataa ccaataccgg cttatccata ttttttacaa
    13261 acaaccgcgc ttgcgctccg gaaacttcga gccgtaaatg gaaccactcg tttttaggta
    13321 tatccacagc gccgataaat cccggcccgc tgtacagttg ccaattgagt cctgtattga
    13381 ggatcggggt atattgcatg gcatcagcaa gtccggattt gtgttggcgg agatataccc
    13441 attccccgtt aaggccgtct tcggatatgc gaaattgtat cccaaaaaaa ccacgcgccg
    13501 caggtgtcga aacgtcaacg tccataacac cgtcacgcaa ttccaaattt tttaagacgg
    13561 cggcgccgcc attgagaaaa atactcttcc gaccttgata atctacaaac ccgctttggc
    13621 cttgcatatc ccatatcgta gaatccatag ccataacctg cgaaaaaaca tttcgagcat
    13681 ggaggaggct tgttaaaaag aaggccacaa taaaaataaa tacacgcatg tcgcacctag
    13741 gttaaattaa tgtaatttat tacgtaaatg gtacgacgtg cggatactat tgtttcaaaa
    13801 tgaatttaag aaaaccgact ccgacgagaa tcacagacac ttattatatt ctaaaaaaac
    13861 attctaaaaa atgaattcga ttcattgccc tgacgtcgtt agtttcttct tggctgtttc
    13921 attccagcga ataaccattt gaccaaattc atcatctctt tttaacgtca taaaacgatt
    13981 ggtctcatca aaaagaaatc catataaacc caacttcata tacaatgaac acaagcgatc
    14041 aaaattactt ggttcaaaat ataacgaagg aaatttggaa attagtcgtt tgagacttaa
    14101 taacgcagtt tccgcatact gatgctcttc aaaccaacgg attgcgcgca tacgctcgga
    14161 caaatacggt acggattgat tttcagcgag cgtttcgtta gcccggccat aggtaagacc
    14221 aaatttttga tcgttggctc gttcaaccgg ttgcgaggga agctgctccg cttcgatgcg
    14281 tgtgatctca gaaactaagt cgcgtttgtt attaacgaac atatcttcga tgtaaaaata
    14341 ccctccggcc ggcgcatcat atacattcgt ttcgcgatag attttgagcg tggcatcggc
    14401 tttagtcaaa acatacgtcc gcggcgctat tttttcttta ctccctagag gctgccactt
    14461 catggatgta acatccagat acatcgcatc gcccttttcg agttttagcc aaatttccga
    14521 ttgccccatc aatggtgagg taagaagaat atagcatatc gttttaaaaa tcatatacgt
    14581 gtaccgttcc atggactact ccgttttttt gaatcacttc atcataatac tggcgcacac
    14641 cttcgcgatg cgcatgttca aaaccgccgt ttacaacggt tgtctccagc ggcaaccaaa
    14701 tcgtcacgcg tccggtttta tcggggcggg caacatattc aaattcggac aaacctaact
    14761 gatccacggc attaaccgga atactcgtat ccacaagcaa aaatacatgc cctatggaac
    14821 ccggtttggc agttggaata ttgatatttt cagtgagcgt cgggcgaata tccaccacgg
    14881 cgcaagcatg gcctatagcc ataagattgg tagccataaa tgcaacaagg tcttcgcaat
    14941 cgccggactt ttgtcgcagt gtctccgaag gatattgcac acgttcattg acaaaagtcg
    15001 tggtagggtc ggatacgtaa cgcaagtttt tcccgacagc cgttacaaat ttttttaacg
    15061 tatgaaactt attatacaat tctgaatacg cggaatcggt tcctatggtc aacaaattgc
    15121 ttttggcaaa cgattgtatt tcaggatcat ccggagctat aaagtagcgc aatccccacg
    15181 tatcgccatc ccagctatgg atatcatata atgtcaccgg aaaactggac agtgcgatgt
    15241 gtttgccgcg tacttcaccg gtaattttca cctgagccac catgccggat tgttctacgc
    15301 tctgatccgg tatgtaaaaa aataacggca ccgatttgat ctcatgtggc gctaaatcaa
    15361 tcgaagatga acgaaatatt tcgccccacg caccggcttc ggcatgtatg acaacgttca
    15421 ggtgttcatc cgacgcatta tgtacatcca tctgtcccac cggattgtat gcataatagg
    15481 cgcgtttggc cgtataaact tttgtctgaa aaattttcat attcaccact tttacgtaat
    15541 cggcagcgcg gcttcctgac aaatcataac gcaatccgat ttgcaccgtt ttgtgaggca
    15601 actgattggt aagttttccg ctcgatatgt cgccgtgacg attaagccat tgacgaaaat
    15661 tttgggatct gaaattttcc gccaaatacc atgtgctttc gtaaccggca agtaatgtga
    15721 agccgccact caaatcataa cttagattta atttccacac ttgataatcc gacggccgca
    15781 aacttctgca atattcattg ttcacctgta acgcatcgta tttcaattgt acattaccgc
    15841 cccaagcatc ccggcctaca cgcgtggtca tcgaatgcga acggtaacga tattgaacat
    15901 ctaaaaaccc gctcaactta tttttcaatg cataatgcat gctgtcgtta cccatgcgta
    15961 tcattcgcca aggcaatatg cctgtcgtta cggcaaacgg accccaatag cttgtcatcg
    16021 caccatagcc taatagtgac gaacggcgat gggatgcaaa ttcataatcc acgtaataca
    16081 ctttcgatgt cagttctttt accaaagttg acacgatttc ttctttttca acgagaattt
    16141 gcgaaccgaa agcaatttca aatcgtgcaa aacggcggcg caactcaaga gccgccggta
    16201 aatcaaccac atacgtgtgc gcatggttag tgatttggct acgatccact aatttaacgc
    16261 tgtcgggcaa caccaacgtc gtggtgccgg gcattatcgt aaagggtagt tgttgattca
    16321 tattcaccaa taccgaaaag ccatacaggc cgtgattgtg catatagtga atccgcgatg
    16381 cgacgtattc ttttgaagca aattggcgag cggtgtactc cgatacaaac gtgaggcgtg
    16441 catcataacg cataggccga tacgcatccg catcgttttg cgcaaaaagt gatgcggata
    16501 caaaaactaa catcaatagt ccccatctag tcatgacgat attccttgat gagttggata
    16561 aggtcatcgt aaaaaaattt aggcgtattg cgaaatcgta aacgtatcac ctcttccgga
    16621 gattcgtttt ttttcacgcc cgtgcgaatg cgttcggaaa tattgttttg atattcatct
    16681 atgcgctgct tcaaagcctt tggttcggat atatccgatg tgccgcgtaa atattcaaac
    16741 atagcctgag gttcctgttc gcaccgtatc atcgccttga tcacccagcc gcgaaaatat
    16801 tcggtcactg tgatgcgatg taaacccaga tcatcggcta ccgcagacat ggcacgatgt
    16861 ttaaattgat ggtcgcggag tgcatggagc acccgctcgg ccaaagattg ctctgaatca
    16921 gaaaacgttg cgccgcgaat ttcgaaatcc gccggtaaaa tataattacg atgccgcatg
    16981 cgtgcattga tcacactgcg ctgagttgct gcttttagct cacgcacatt gccgggccaa
    17041 gccaatgtgc agagccattg gacgagatga tcggagtatt tgagttccgg agcaatttcg
    17101 tacacaaact gacggaatag aaaatcaata tcaaccgtac gttcgcgtaa aggtttcagc
    17161 ggcaacacaa aaccggcaat acgataatat aaatcttcgc gaaactgttt atcccggatc
    17221 aataccgata tatcccgatg ggtcgccgca ataacacgaa ccgatacgct tttcgaaaca
    17281 acgccgccga cgcgttcaaa ccgaccttcc tgtaaaaccc gaagaagttt aacctgaaat
    17341 gccgggcttg tctctgcgat ttcatccaaa aacaacgtac cattatctgc catttcaaac
    17401 cggcctggct tggcttgtac cgcgcccgta aaagcgcctt tttcatatcc aaataactcc
    17461 gcttcgatca aattgtcgtt aaatgcccca caattaacag ctacaaaagg acccgaagcg
    17521 cgctgtgaac ggcgatgaat aaattcggcg atacgttctt tcccagtgcc ggattcaccg
    17581 gtgatcagca ccggaacatt tgactgggct acctgatcgg ctttttggag aagctctgcg
    17641 acgggcgaat ctttatggta ataaataccc tgcgcttcac ggcattccga caaatccaac
    17701 gggcgaattt gatcggatat tttttcgtaa aaattgagcc gataccttag atccgtcaga
    17761 tcatccgatg cggcagaggc aacctctttt gtcacagtaa cggtgcgacg cttgatcccc
    17821 aacatgaacg tcgaaacgag tatcggcaca agagtccaac ctataccgat aggcaaataa
    17881 atacgcaaaa agtatagacc ggcagcgaac gcacaccaaa tcaatgccgg ggcgcctatc
    17941 caaatccaac gtgtatcacg aagcgacaaa aagctcacta ctgccgtacc ggcgaaaaga
    18001 acaaccagca accatagcgg ccattgtttc cacatacgac cttgtttgat ttcctccgca
    18061 gcccatatct gaataagact gacggggtta accgaacctt gaggtgacgc tacatacgag
    18121 gttacaccgg gatagtcaaa ataaaataca acacttcggt tgctcaaatc cgaattggac
    18181 gacaaaaacg cactcagatg catatccgga agtaaagtat cggatggcca gtgcaatata
    18241 acggtttctt caggtacttc tgttaaagct gaaaccagcg aatgatcacc cggcgtatag
    18301 atcaggtagg cattttcgat ttgccaatgt gccgcaaacg gaagaaatgt ccgaccggca
    18361 ggaaacgatt ggccgccaag tatagtatta ttttcctgaa cacgttccgt cataagaaaa
    18421 atcttatcat cccttacgca ctcatgataa aaaacttcgt ccgactccgg atgcaacgcg
    18481 tcggccgagg gaaacgccat atcaaaatag atcgcttgcg cgccgttttg tttagcatgt
    18541 tgtgcggcga tcgcatacca tcgtcgagcc aaaggccatc cacccagatg ctctacatcg
    18601 tgtttaccga tagcaatccg gatcaccggc cgttcctgcg ccgtaagtaa cgaattgcaa
    18661 aacaaaatca aactaaggca aacgacgatc tttttcataa aataaacggc gctgactttt
    18721 gtagccagcg ccgtaggttc tccgttattg ttttttcaat ttgactaaaa cattatgcgt
    18781 ataatcaatc tcaaccggca agccatatga ttggccatta gttttatcaa tcgtattgat
    18841 tgaaccaacg acattttcct caaacacaaa taaatgatat atcacttcat cttgattcca
    18901 cttggatatt agctgttcta agtgttgttt tgaaattact acttcatccg taggtacagt
    18961 ataatcaaag aaagaatata tgtaaccgac cgatgttgaa tccccaaatt taatgttttg
    19021 gggaacaaac ccaacaaaac ctgccggaga aacccggtta agttttaagt gtaaatcttc
    19081 attatcgcca atggtttggt tgaatgtaag attgataaat ttgagttgcg ttgggacaga
    19141 aatatccgta gaaaaattat taatatgtgt ggaattagaa ccggatattg tcagtttatt
    19201 attcacagga tccaacggaa gggtcaacgg gaaaccatca tatcgtgtgg cgtcaactgt
    19261 atctttatgc gccggaattc cacccgaatc cggaacattc acgacatagt atgtatgata
    19321 tctttgtgct gcaaaaccgc cgacattcag cgtaccgtaa tctaaatact ttttgagtgt
    19381 tcgtccgttt atattggtgt tgaggtcatg ttcgagaaac ataaaatatt tcgaagtact
    19441 actactactt gtatctgtat tgcctataaa gacatacaac atactatctg gtgggggtaa
    19501 aactgctcct attgtgccgc ctttattcgg atcatgcgag ataataagcg tttgatcaga
    19561 accgacgtcg gatttgacga tcgccccatc tttgtcgcaa ccgatcgcca acaaagcgat
    19621 ggttactatt agcgctactg attttgacat acattactcc ttgttgttgg ttaatacttc
    19681 tgtgtaccaa ccatcaacaa accaatttca tgccaacgca taactattta ttttttttag
    19741 atatgctttt acaaaaagtg cagtgaccac tgaactgaag tgtagtggag ttttttcaca
    19801 acataaatta acacgattta tagcttacca caagcgataa tgtgttctgt atagtttttg
    19861 attcagtcca catacaggaa ggatcacaaa agctttaata aaaaagcccg tttaccttgc
    19921 gataaacggg cttgaatgaa aaaaactaaa ttctaaaaaa ttccgcgtat gacgccgtta
    19981 tcatccatat caatcgcctc cgatgaaggc accgacggta acccgggcat ggtcatcatt
    20041 tgcccggcaa tggcataaat gaaacccgct cccgcagaag ctttgatatc atcaatagga
    20101 accgtaaaat ctttcggacg ccctttgagt gtaggatcat gtgaaagtga aagatgcgtt
    20161 ttagcaatac atatcggcag tttatcaaga ccttcttctt tgagcatttt aattttcgat
    20221 ttggctttgg acgacaggga aatgcccgca ccgccataga ttgtttttac gaccgtggcc
    20281 attttatttt cgatcgtacc ctggacatcg taggtaaatt tcacttccga tttttcatcg
    20341 caggctttga ctacttcttc ggccagcgct gttccgccta ggccaccttt gccccatacg
    20401 tcaatcggta tcaccgcacg cgctccggtt ttaacggcaa tatcgcgaat ggcttgtatt
    20461 tcgttctccg tatccgaagt aaatcgattg atcgccacga cgaccggtac accaaaactg
    20521 cgaatatttt cgatgtgttt tgtaaggttt tcacaacctt tgatcacggc ttcgacgttt
    20581 tctttttcca ataatgcctt atcaatcttc ttgcccggaa cggcttcaaa tccgccgccg
    20641 tgcattttga gcgctttgac cgtagccacg accactgcgg ccgagggacg caatccgctt
    20701 tgacggcact tgatattgat cagtttttcc gcgccgcaat ccgcgccaaa accgcattcc
    20761 gtcacgacat aatcggcaag gcgcaaggct attttatctg caataaccga attattaccc
    20821 gtggcgatat tggcaaaagg tccggcatgc atgatcacag gaacgccttc cagtgtttga
    20881 acgaggttcg gtttgatggc gtctttgagt aaaaccatca gtgcgccgac gacattcagt
    20941 tggcgtgtgt acacgccttt tttatcccgt gaagtgccga caagaatcgt ctcaagacgt
    21001 ttacgcaggt ccttgaggtc ttccgcgagg gacaggattg ccatgatctc actggcggcc
    21061 gtgatatcaa atcccgtttc gcgcgggata ccgttattgc tgccgcctaa tccgacgatt
    21121 atatcgcgca acgcgcgatc attcatatcc atcacgcgac gccagtatat attattgatg
    21181 tccagattca gcggattacc tttgtgcagg tgattgtcga taatagccgc aagaagattg
    21241 tgtgccgtcg tgatcgcgtg aaaatcaccg gtaaagtgca tattgatatc ttccatcggc
    21301 tgcacttgcg aataccctcc gccggtcgca ccgcctttga caccaaaaac cggtcccatc
    21361 gaaggctgac gtaatgtcga gatcacattt tttcctattt tggccaacgc ttgcgacaat
    21421 ccgatattga cgacggtctt gccttcaccg agcggcgttg ggttgatcgc cgtgacaaaa
    21481 ataaattttc caattttttt gtccttcaga cggtccaata cttctagttt gatcttggct
    21541 ttatgttcac cgtacaactc aatatcggag gcattcaatc cgatcttttt ggcaatttca
    21601 gtaatcggcg taatgggtga ttcctgagca atttcaatgt cgcttttcat atatcaacct
    21661 cataatagat gttttcgatt ggtcaattct acgatttcga tatatacgat tccatgacta
    21721 aagtcatgtc taaaaaaggt aggttttccc gatctaaaaa gaatgctctg atatcaatgg
    21781 tacgtttagt ggcgattcaa aaaatccatc acgatttgac aaaatacatc ggctttttca
    21841 tgatgaatcc aatgcccggc atccggtatc atctctaatg ctgacgaagg aaaatgttta
    21901 gctattagct ccgcgtcatc acgaagatta tacatggatt tctcaccgcc gacaaatagc
    21961 gtaggaccgt gaaacctatc gttttccgag gctttgaatc gtactatttc attggctgca
    22021 aattccgtca aagccggaag attaacacgc caatacatcc gatcgtgatc acgcaccagg
    22081 ttggtaatca caaaggatcg tacggccgga tttttgattc cctctgcaag ggccacttcc
    22141 acttgttttt tttctttata aagattcaga tccacggcat tcatcgctgt gagaatttcg
    22201 atcaaacccg tgccgtatga acgcggtgca atgtcttcta cgatcaacgc tttaactcgc
    22261 tcgggatgtg caaaagcgta attcatcgcg acatggccac ccatcgaatg gcccaacaag
    22321 taaaattcat gcaaatgcaa acggtcaacc aacgcctcaa tatctttacg catcgccacg
    22381 atagaatgtt caggatcgtg cggcgaaact ccgtggttgc gcagatccgg aatgatcaag
    22441 cggtaatggt cggataattt tttggctacc gaataccagt tgcgttcact gcccagcagg
    22501 ccgtgcagta tcaccagtgt tttcgggctg ccgtattcga caaaatgtaa tttcatacgc
    22561 tccgttatat tggcgcggaa gttagtttcg cttatatcca attgcaacgg tttttacaaa
    22621 acgaaggatt attaccgaat cggcttgcgc ttggtttggc gctgttctaa tttaaagaaa
    22681 aaacaacaga tcaatctatg gacattactt acctcacatt cgttttaggc gccgtcgtta
    22741 cacttttgat ccttgcgcgc ctcgtcaatc aatttctcat gcgggaacgc ctcaccgatg
    22801 cgctaactga aaaagatcgt ttggcaaccg gtatcgcact ctcgggctat ttgttcggcg
    22861 tgatgatgat catcatagac gtactcagcg gcgaagggca taacgattgg ctcaaagatt
    22921 ttttacttgt cggagtttat ggattggccg ggatcgtatt tttggttttt gtcagtgtga
    22981 tggagctgcg tctgatcctc tccgccaaag cattggatgc cgttcgtgaa ggcaatgtcg
    23041 ccgccgccat tacggtcgct gcttcataca ttgcgacatc gcagatcatc ggcgctgtcg
    23101 tatccggcga taataccggc ggcaattggc tgacggcagt tatttttttc attatcggtc
    23161 aggccacgct gctgatcatg acgtatgtat ttcggtggct cacggcgtat aacgatgccg
    23221 aagccatcat gcaaggtaat gctgccgccg cgctttcata tgccggggtc atgatcggta
    23281 tcgctattat tgtaggcaca gccttacgcg gtgacttcat agactaccct accagcctta
    23341 tagattatgc atggtcgctg gtgatcgtcg tggcacttta tccgatccgc caatttttag
    23401 tgcaaggtgt tttactcgga tgcggattta gtttttatgg cggtaaatta gatgaagaaa
    23461 tttccaaaga tcgcaatatc ggcgccggat gtattgaagc aacgacctat atcgcctcgg
    23521 cacttttagc cacggcatta ctgtagtaac attaaatcta tgcgcacctc cgaggtaaaa
    23581 tacaccgatc cttcggttcc gcgacgcgac ccgtatttcg attttgcaaa aaaaatcacg
    23641 gattcgcata tcctcaccga tccttggatt gacggcgatc cgcgtttttc ggacgaaccg
    23701 gtgatcctcg gcgccggaac ttatgcattg atgcaaaaag cggccgaaca catcggcgct
    23761 ttatatgacg aattggtgcg catcattctt cgcgaaccgc attggctcga tgattatttc
    23821 catttgacgc cgcatcaaaa gatgctttgg tttgcctccg gcggacgatg gcatggcatg
    23881 gcgcgactgg atatgttttt atgtacggac ggacgtctgc aagtatgtga aatgaattcc
    23941 gatacgccca gcggtgaagc ggaagccgtt ttgatcaatc agattattcg atcgcgcttt
    24001 ctgcgtttta ccgatcccaa tgccggattg gaaacgcaat ttatacgtat ggttttacgt
    24061 ctctggggca aacgtacatg gcccaggctt tctccgacgg tcggcatcgt gtatccgacc
    24121 gaaatgcccg aagatctctc catgatacgc ttgtatcaac gttggttgga atcccgcggc
    24181 gctcatatca tattgggttc gccctacaat ctgggcagca ctgcgcagcg gctgacgtta
    24241 ctgcaaaaac ccgtggatgt attgattcgt cattacaaaa cagattggtg gtgcgaacgc
    24301 ttgccggtgt ggaaagatca ggaacctttt cccgacgcgc ttccgctcgt caaaccattg
    24361 caacacctga tgcgcgcctg gcttgcggga cagataacga tgatcaaccc gcccggcgct
    24421 gtgcttacgc aaaacaaact gacgatggct tttttttggg agcacctgcg taagttttca
    24481 cctacggcgc ggaatacgat acgcatgtgg attccggaaa cgcgtcgcct gataacgaag
    24541 gaaagcaaaa aactgaataa aaatgaatgg gtactcaaa
//
LOCUS       DAOQGF010000042        23507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_994_length_23507_cov_6.648644, whole genome shotgun sequence.
ACCESSION   DAOQGF010000042 DAOQGF010000000
VERSION     DAOQGF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_23507_cov_6.648644"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PK937_07650"
     CDS             complement(<1..869)
                     /locus_tag="PK937_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH32481.1"
                     /translation="MLQDLAVQSFLMGLLSAASLPLGAIIAMYWTPKDRVVAAMMAFG
                     GGALLAALTIDLVAESLRKGEFYPLAAGCIGGGLLFVLLNKIVNDNGGFLRKAATTMQ
                     HLNKLKLRSYQSFYRRLSKVPLFQILPPEELTALVRTVKTHRFKKDECILRQGDPGES
                     MFVIQSGDVLITDPKQNNRTIATLRASDVFGEMALITGEPRTASATAQTDVLAWEIHK
                     KDFDFLIRQSPVLAAAMHSLVETRLADLKEHGPVSDEHAEAWYDDATHHLEAHMQPPT
                     AQEVKQVSESHGGA"
     gene            complement(972..2327)
                     /locus_tag="PK937_07655"
     CDS             complement(972..2327)
                     /locus_tag="PK937_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNH32482.1"
                     /translation="MKKWFTLDSKLSTEVLHLALPVVAGLASITAIGLTDTIMVGRLG
                     ATALAATGQAVMVFWTVNWVLRSVELATQAISARRFGEGQLPACGEVLHHALVFSLTL
                     SFFSMIVLYSMAPRLTRWISANEEVASLATGYIRVLFATLWLSGMFFALRGFFSGIGR
                     TRVFLMSAGILFTANLCGNYMFIYGHWGAPAMGVSGAAVGSALSMICGFSFLILYTFW
                     HRKKTLAAYELFSGAKWRWELVGEIARLAWPNMFRGVMVIGGLLVFYAMVDRVGVAQA
                     AVVNLVINIQSVSFMPGYGFGIAAATMIGHSLGAGNTERAERAGYEAMKLGIIFMGSV
                     GILFLVAPEWVVRVFTDDEEVMKYAIGPLRLVGIVQIVDAAGMVLSTALEGAGQTRWV
                     MMMEILVNWGVFLPLTYLLTFTLNMRNYGPWIAWGVYITLFGVISFIKFNRGSWKHVR
                     L"
     gene            complement(2639..3883)
                     /gene="ftsZ"
                     /locus_tag="PK937_07660"
     CDS             complement(2639..3883)
                     /gene="ftsZ"
                     /locus_tag="PK937_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNH32483.1"
                     /translation="MITYEEAREYGAKMRIVGIGGGGCNAVNTMIASTLSGVEFVAIN
                     TDVQALERNQAHHKIQIGKNLTRGLGAGADPELGKRAAEEDMDQIKAILDGTDLVFIT
                     CGMGGGTGTGAAPIVASVAKSMGALTIGIVTKPFNFEKEKRMRQALRGVEELRKNVDT
                     LIVIPNEKILMVMPKNTPFIQAFRTADDVLYRATKGIADLISNTGYVNVDFADVKRVM
                     SGAGDAIMGMGVGSGEGRGEMAVKQAINSPLLEETSIEGATGVLLNLTCPQDFTTEEV
                     DQISNMITGVAGEEADVIWGYSVDENLKNEVHVTVIAAGLNKHRYTKSVAPEMPTSAS
                     EQAQQPMTLRTKKNPHDYYEHLKELKEQKYGKLDQPAYERMKESEPVAEEDDFLSKTG
                     TDAQDDSAIPAFLRNAARQRKM"
     gene            complement(3932..5257)
                     /gene="ftsA"
                     /locus_tag="PK937_07665"
     CDS             complement(3932..5257)
                     /gene="ftsA"
                     /locus_tag="PK937_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNH32484.1"
                     /translation="MKNRIVVGLDIGTTKIAALIAEVGGKEIKIKGVGTCPSVGLRRG
                     MVVNIEHTVESIKKAMQQAELMAGIQAESVYVGIAGDHVRGINSHAVVAVGRDDREIT
                     EDDVRRVIDAAKAINMPMDREIIHVIPQEFIVDKQEGIQNPIGLTGTRLEVEAHVVTG
                     AVASAQNIYRCVERAGYKVKDLVLEPLASSYAVLETDEQELGVVLIDIGGGTTDIAMF
                     FDGSIRNTDMLGIAGQAVTNDVSICLKIPYDKAEEIKKKHGCAMASLVDETESILIPG
                     VGGRDARRENRQFLSKVIEARMMEIFNLVRSVIEKSKLRSMMNAGVVLTGGGSLLEGS
                     AELATEIFQLPVRIGSPRGFGGLMDAANSPIYATGIGLIHYGIQKEKSGDDDMEDEGD
                     ETPPEGPTEGDPGETHTDALEMQSQTGEYQAPLYQKISKKMKRWTEEFF"
     gene            complement(5282..6661)
                     /locus_tag="PK937_07670"
     CDS             complement(5282..6661)
                     /locus_tag="PK937_07670"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNH32485.1"
                     /translation="MIRKKREVAQVDLFVKENILPEEPSFLSKSAETGHTENKETVIK
                     KIQIERTFKNPILMGEFNGTAEHKKDESTENAVREIHKRDEVTAEEISIRASVSQPTE
                     EISLDKVFGRSEQAEADAFPAAAEEVKENSGSYDEIIAIAAEEPQPQAVTLSENSEDE
                     SQEAVEVHTKPGIIPKEAKPKIINRDFSEGFYLTEEARIKAEEAKRARKQERRARRIN
                     KLAVKTVSVLLLLFFVMVLFNWQEWVVNSSLFSLRNVYVQGNLIGSKEDIIKAADLDM
                     GRRLAEIDLTKTADRIKMNPLFEHVTVSRNYPSSVVITVKEREPFAFLPMDELYPVDA
                     NGFVLPKLKARMIYNLPVISGMTGVAQPGKKFKNDRLPLALQFLAKAKEADESLFYDI
                     SEVVVNKKDMTVFLNSLHVAFRIEDEELMRAAVYLSAAAQHFKQEDDAVKGTREVDLR
                     YNGQIFLRN"
     gene            complement(6693..7622)
                     /gene="murB"
                     /locus_tag="PK937_07675"
     CDS             complement(6693..7622)
                     /gene="murB"
                     /locus_tag="PK937_07675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNH32486.1"
                     /translation="MKTTEDIRSGFRGRWTENVLLSDKTWYKIGGPCIAWCEPDDIED
                     LKYLLRMCKKNEIGFFVHGKGSNLLVSDRGISGIVIDLEKACSFTQFEKTQVSAGAGV
                     YMPKLVLECERQGLAGIEMFAGIPGTVGGAIRMNAGCHGKEMFDVLTEVTTLEDDVLV
                     TRSKSEIRFAYRHVETLEKPDTVIVSGKMVLEYGDKEALAEQRKAYMEKRRLTQPINL
                     PSSGSVFKNPAGHHAARLIEEAGLKGFRIGGASVSEKHANFIVNEGKAKAADVLEIIK
                     HIRQTIAQRNGIRLETEVKFVGFNHEELDGITD"
     gene            complement(7619..8980)
                     /gene="murC"
                     /locus_tag="PK937_07680"
     CDS             complement(7619..8980)
                     /gene="murC"
                     /locus_tag="PK937_07680"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNH32487.1"
                     /translation="MIRPIQHIHFIGIGGIGMSGLAEVVFRQGYKVSGSDKAFNEQTA
                     HLQKLGVTCYEGHRAEQVHADLVVYTAAVHEDNPEIIEARRQHIPMIKRSALLGEFLR
                     GKKGVAVAGTHGKTTTSAMIGMMLEHAGLDPTMFIGGVVRQLGTNAKLSDSAWVVVEA
                     DEYDRSFLELHSHIALINNIESDHLDIYRDLDDITGAFTQFAKQTSFFGAVIGNADDP
                     AVVEVLRQCGKRTMTFGLSEAADLRAANIETKFCELHFDVLLQSKKIERMMLPMSGTH
                     NIKNALACIATGLMCEVPVARIAESLAKFGGTGRRFERLGEKDGVLFIDDYAHHPTEI
                     RATLEGARDGAGGRKIIAVFQPHLFSRTRDYFEDFASAFTAANEVILTEIYPAREMPI
                     PGITGEKLYETTKKNHAHVTFIGDKTKLSEAIRNAARPGDIVITMGAGDITHIGRQMV
                     RGV"
     gene            complement(8977..10089)
                     /gene="murG"
                     /locus_tag="PK937_07685"
     CDS             complement(8977..10089)
                     /gene="murG"
                     /locus_tag="PK937_07685"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNH32488.1"
                     /translation="MAWRVIIAAGGTGGHVFPALAIGEEIRAQKPETEILFIGTNRGL
                     EERVIPAAGFRLKTIVMSGVQRSLHPRDVLRNLVMPFKVLFGLLQSITVMWTFRPHAV
                     IGCGGYVTGPIVLLAALLGKKIILQEQNSRPGRTTRSLARWADAIHLTYEDAQRFFDP
                     KCKLMMSGNPLRKGFVRQDMAAARAALGLASERNTLLVLGGSLGARAVNAALLKIMDT
                     LLQDDNIQVLWQTGKPDYENVRYTMKPYSQVQVHAFIDNMIAAYSAADTVLCRAGAMT
                     LSEITMMGLPAVLVPYPFAADNHQEYNARSLSDRGAAMTLLQTDLNEQLLPALRKMMQ
                     DQAYRRAMAEASYALRQPDAAQNIVRHMFSLEGVRA"
     gene            complement(10089..11378)
                     /gene="ftsW"
                     /locus_tag="PK937_07690"
     CDS             complement(10089..11378)
                     /gene="ftsW"
                     /locus_tag="PK937_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNH32489.1"
                     /translation="MTTETLTPRKTIDKGLFLIVLTLMLFGIVMVYSASNVIAEQKFG
                     NSTHFLVNQFLRAMMGIVILMFAAKYDYHNYKRHTMMMLLIAGVLCAVVLTLGQLKGA
                     ARWLQVGGFGIQPSEIAKIALVFYVAGYMDSKGDRIRDFQNGLLPPLIMAGVFAVAII
                     LQPNFSTAAVVMGMVILMLFIGGMDIKHLGMMAAVAIPVALLTVILSPYRRARLMAFL
                     DPTADMQGSGYQIKQSLISFANGGLTGVGVGQGQQKLLFLPEPFTDFIYSIIGEEMGF
                     IGAVLVLGLFIVFLFRAIKTARTAPDMYGFYLATGIIISIVVYALVNAGVAVGVFPTT
                     GLPMPFISYGGTSLLFTCFLVGVLLNISSQTVSKDEAMKATAPEYAMRSRFTENFNVG
                     GEIGEEEEYPTDTTPVPTKKAPAKKAVRTNIVGMDMS"
     gene            complement(11382..12734)
                     /gene="murD"
                     /locus_tag="PK937_07695"
     CDS             complement(11382..12734)
                     /gene="murD"
                     /locus_tag="PK937_07695"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNH32490.1"
                     /translation="MRDVKGKKITVIGAASSGIAAARLLKHKGASVFVSEFASEEKKN
                     EAKVLLQNEHIACEFGGHTERATDCDWAVVSPGVPSDIPVLQTLHARNIPVYSEVEIA
                     SWWIPAPVVAITGSNGKTTTTTLIGELFKHAGRTTVVAGNIGTPLSDCTETLPKDGVA
                     VVEVSSFQAEGFESFHPQVGIMLNLSPDHLDRYSGVEAYYAAKKNMIKNQTASDVLVV
                     NQDDATLMQLIGDSKAQRWAFSLQSIPENGAYVENDTLMVKINGKVEPVIATGDIGII
                     GRHNLYNAMAAILAAKALDVKTSVMAETLRSFRGVEHRLEFVAEKSGVRYYNDSKATN
                     VDSTFVALDSFKKEKVILIAGGKHKGSPYTPLKTLVEARVKSLVLIGQAADIIAGDLG
                     AVTRVQRAASMQEAVEISKQTATPGDVVLLSPACASYDMFKNYEDRGRQFKMAVEGLK
                     "
     gene            complement(12739..13845)
                     /gene="mraY"
                     /locus_tag="PK937_07700"
     CDS             complement(12739..13845)
                     /gene="mraY"
                     /locus_tag="PK937_07700"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNH32491.1"
                     /translation="MFYFLYQIFADSGISGSNLFRYITFRSAMAAVTALVISLFVGPF
                     IIRKLREMQIGEEIRLDGPQSHLSKAGTPTMGGLIILASTLIPTMLWADLSNTYVLLV
                     LLATAWMGVVGFIDDYLKAIKKMKKGLVARYKTAGQISLGLIVGLVMIMTPEFERFAT
                     VTTIPFVKDLNFDFGYFYIPMVIFVITATSNATNLTDGLDGLSIGVVSIAMTAFAVMC
                     YVTGNVNFSDYLNILYIPQASELTIFCTAMVGAGLGFLWYNAYPAQVFMGDTGALAIG
                     SALGTLAVLIKKELLLPIIGAVLVAENISVIAQVMYFKYTKKKYGEGRRIFLMAPLHH
                     HFELKGWHETKVVTRFWIMAILCALLTLTTFKVR"
     gene            complement(13858..15225)
                     /gene="murF"
                     /locus_tag="PK937_07705"
     CDS             complement(13858..15225)
                     /gene="murF"
                     /locus_tag="PK937_07705"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNH32492.1"
                     /translation="MPVFTPADLKAIGECRNILDTWSASGVSTDSRDAQTSQMFFALQ
                     GARFDAHAFVHDVAARGIVCVVTAEAFARYASAWEKLPLVIVQDTLHALQRLAENHRQ
                     KSRAKVIGVTGSNGKTTTKEMLKAVMSKTFITQATEGNLNNEIGVPLTLLKLEADTQV
                     AVIEMGADRPGDIETLCRIIKPDFGIVTNIGKAHIERFGRVEAVAETKTALYRHLINH
                     GVRFVNTDDPFLRDQTELAGDLVTYGVNQSAQFRGTILEVSDKGCISLRIEADKSPAM
                     LIRLGVPGLHHAYNVLAAVAVAKHMPMPDEAIVQALEAYRQPANRVSFKEKNGITVMD
                     DTYNANPNSMRAAMDTLMSIKTSGRKIAVLADMLELGAMADNEHRDMGAYAAEKKVDA
                     LYLTGPLAVHAMDGAQGMKNKFYFEDKARLIEMLKDAVQPGDVVLVKGSRGMKMEDIV
                     QAILA"
     gene            complement(15232..15420)
                     /locus_tag="PK937_07710"
     CDS             complement(15232..15420)
                     /locus_tag="PK937_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32493.1"
                     /translation="MDFEKKKEELGFRHFDPKTFEPGVFSHNELLSDIRNNPDKLDVH
                     SKEYFDETNYKLFPKKDQ"
     gene            complement(15437..17629)
                     /locus_tag="PK937_07715"
     CDS             complement(15437..17629)
                     /locus_tag="PK937_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNH32494.1"
                     /translation="MPKQKNMTPKPEPKIQPKTDHASFVKTRVERLRILAFGLVAFAL
                     VIVGRLFFVQVANIGDYEAMAKRQYERRAVLEAERGVIYDRNMQKIAVNLINYSFAAD
                     PSFMNEQDKARVAENFSRVFGKPKNEYLKRLNKPGAFVWLERRVNDQVAEKVNDSVKG
                     LIKLQSLRRHYPFGKAGAALLGMTNIDNEGSAGIELDQQATLGGKDGWAILQADALGR
                     LYPNPDYPHEDPVNGKDVVLTVDMNYQMAAYQVLEKTVKDYNADNATAVLLSPQTGEI
                     LAMVNYPGLDPNAPETFDAELLRNRAITDLYEPGSAFKAFSAVAALEEKVRNPEDRIN
                     CEWGKMTLYGHVIHDTKKHGTISFTEVVQKSSNIGIIKTTDLIGSDKLYQYVRAFGFG
                     NETGVDLEGETRGLLSHPSTWSGLSRPMISIGQEIGVTPLQMVTAYSAIANGGTLYKP
                     YIIKGVHDPLERTQTEHETQLIRTVASQETMQRVSRMLKEVVEGGTGHRAAIKGIEVA
                     GKTGTAQKIDANTGRYAPNSYVASFAGFFPVQNPQVAFIVTVDNPRRSIWGEASAANA
                     TRQILEKIINSSDDFAKHVNRVLADLDQDTTAGDAAMTAPDVKYLNIAAAKNVLDRMN
                     LEFRIAGTGDMVTTQEWTYDPKLKQNILLLTTGNAVTVSESDTLDFRKKRIPDVRGLS
                     MRMAMNKLYESGIDVKIKGNGYVIAQYPRAGAVAKNGESVIIQCKPNL"
     gene            complement(17601..18152)
                     /gene="ftsL"
                     /locus_tag="PK937_07720"
     CDS             complement(17601..18152)
                     /gene="ftsL"
                     /locus_tag="PK937_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNH32495.1"
                     /translation="MSTYSGNIVGVDFSGRKKKTTETPVSPAERTKTEDRYARYSKAP
                     VKSSARENETVRVEAKPAVQETVASAAVEASNKKGITPLRMFLAFVVVAILMLVWVWM
                     SVTVRQEMLMLEQLKDKQLETQKANESLQAEITRLSNYERIAKIAKEQLKMKPATEKP
                     GVIFLDTEKQKMLQDAEAKKYDP"
     gene            complement(18179..19102)
                     /gene="rsmH"
                     /locus_tag="PK937_07725"
     CDS             complement(18179..19102)
                     /gene="rsmH"
                     /locus_tag="PK937_07725"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNH32496.1"
                     /translation="MPDYHIPVLLQSCIDFLQIRPDGFYVDGTLGGGGHSAFILQQND
                     SCRLFAFDMDRDAIKAAGIRLAEYGDRATLINDNFRHVRSALAARGITQINGILLDLG
                     VSSHQLDWSDKGFSYRFEAPLTMAMADDTAFTARDVVNGYSEQELARIFFHYGEEQQS
                     RRIARTIVQQRTTTPLQTTKDLADLIRRIIPERHANKTLSRIFQAIRIEVNDELNNLR
                     QALADATGILAIGGRIVVMSYHSLEDRIVKDFFRDEAMDKILNTHYPELSEPKTPRLK
                     VITKKPYEATDEEIQLNPRARSVKLRVAERI"
     gene            complement(19106..19543)
                     /gene="mraZ"
                     /locus_tag="PK937_07730"
     CDS             complement(19106..19543)
                     /gene="mraZ"
                     /locus_tag="PK937_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNH32497.1"
                     /translation="MPSFKGTYNHILDSKNRFNIPAKMRSAFVPEDKDTVILTRGFEQ
                     CIYMYPYSEWLRREDELRQLSNMDSDARKLVRLLMGFAQECELDKQGRVIIPQPLLSF
                     AGIEKEIVIIGALERMEAWNPKQYDAAHNGFDLETTAAKFVKF"
     gene            20033..>23507
                     /locus_tag="PK937_07735"
     CDS             20033..>23507
                     /locus_tag="PK937_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH32498.1"
                     /translation="MKRFMYDFLQKNAAMLCVLLCCSASLFAQNNALTLNGTSQYAYY
                     NGSLSTYEDVTMEGWFKWDGSTGNNQLLMYDGHTSLNGFGLMLDASASNNLIILAGGY
                     QVTSVGRGLDVGEWSHVALVKKGNFFSLYINGDPEIAKAMTYSYPSGSYMVGGNSINN
                     SEFFKGAIDEVHLWNRALSYNEIFTAAATPMTGAETNLVASYRLDNSADGLTGDQTTN
                     GYNLTLVGTPTFTASSSDSVMIRQQTKSLYTGGTSVSVGWHADPHSFVTGYIIYADTS
                     DAVRTQVGTTSSRTDTTFTVTGLLPNTVYYLTARAVTATDTSDYYRLAVVSTFESSGR
                     AAMFSDNNYYAAPFNENIPIGSSAYTIEAWIKPNSMGVHGIVGWGQYGNSYQTNALRL
                     TEDGIINYWWNNDMTISTGNIAGSWHHVAATYDGTTRKMYFDGKLMASENVVYGPEIP
                     FSHNFTIGVTNTSEYFNGEIDEVRIWNVARSESEIVSLLFSPATGSESGLASVYHFDE
                     IVGDYAGDYKNANHAEAVSGTPQRTLSDAMLPFMPARLQAIAGDGQVELRWEKSIHPD
                     FASYTLMAATDIEFTDIVATHSSSAGNASDTSHTVAGLTNGTLYYFAVKMTTNSGATT
                     PFRMNAAVPNTGGPGNALIFDGEDDQIILEDNLVRSRSEMTIETWFKTTTSGGIFGYN
                     DASNAEHVPVIYVGMDGYLQSQLWIGDYNSVMRSLRPVSDGYWHHVALTAGNDNQYLY
                     IDGQLCDSIIGRPLNHLSMIKNQIGRATTSGWPNGGNEYFSGELDEFRFWNRALSHQE
                     VYSYANKSLRGDENGLVLLHHFNEPSGLIYTYDAGPNGWTGGLESFSASTTVASGALR
                     PFSPYNLQSTNTLTSITLKWNRSNAASVRAYYIYTFDGESTMLWDSTTGGLTDTTITF
                     AELPSFTNFTYYLSTVDASGSESEKSNPIFVTTTSFKEVTGTTFSQVVDGKFQWADYD
                     NDGDYDVLVAGSENNGYYPPYQNGTIKLYRQDNGGTFTLVDVGLTQEIHRGTVAWCDY
                     DNDGLPDISYMGSGGNGTRPFKLYKNDGDGTFTEVLTAIQGALFGAMAWGDYDNDGDQ
                     DVFVMGSIGSEGGDNYSMLFRNDRNDLFTQVDIGLIGYSGGSAAWGDYDRDNDLDLLI
                     LGSTNGSYTGGD"
BASE COUNT         5886 a         6242 c         5173 g         6206 t
ORIGIN      
        1 gcgcctccat gcgattccga aacttgtttg acttcttgtg ccgtcggagg ttgcatatgc
       61 gcctccaaat gatgcgtggc atcatcatac cacgcttcgg catgttcatc actgaccgga
      121 ccgtgttctt tcagatcggc taaacgcgtt tctaccaaac tgtgcatcgc cgcagcgagc
      181 acaggtgact ggcgtattaa aaaatcaaaa tcttttttgt gaatctccca cgccaacaca
      241 tccgtctggg ccgttgcgga agccgtccgc ggttcaccgg tgattaatgc catttcacca
      301 aacacatccg acgcacgcaa tgtcgcaatg gtgcgattat tttgcttagg atcggtgatg
      361 agcacgtcgc ctgattgaat gacaaacatg ctttcgccgg gatcaccctg gcgaagaata
      421 cattcgtctt ttttgaaacg atgtgttttg acggtacgta ccaacgcggt gagttcttcg
      481 ggcggaagaa tctgaaacag tggaaccttg ctcaaacggc gataaaacga ttgataactc
      541 cgaagtttaa gtttattcaa atgctgcatc gtcgtcgccg ctttacgcag aaaaccaccg
      601 ttgtcattga cgattttatt cagcagaaca aaaagtaatc cgccaccgat acaaccggcg
      661 gccaacggat aaaattcacc tttgcgtaac gactcggcca ctaaatcaat ggtcagcgcg
      721 gccaataacg cgccgcctcc aaacgccatc atcgccgcaa ccacgcgatc tttgggtgtc
      781 caatacatgg cgataatcgc tcccagaggc aacgatgcag cgctcaaaag tcccatcaga
      841 aatgattgaa ccgctagatc ttgtaacatg atttttatcc tcgattaaat agatcaacga
      901 gaggtgtcaa agcgaagtgt gatggattta ttgtactaac aaacgatgaa aagtgcaatc
      961 gctgcattcg tttacaaacg cacatgtttc caagagccgc gattgaattt tataaaacta
     1021 attacaccaa acaatgtgat gtaaacaccc cacgctatcc acggcccgta gtttcgcata
     1081 ttcagagtaa atgtaagcaa ataagtcagc ggcaaaaaaa caccccagtt aaccaaaatt
     1141 tccatcatca tcacccatcg cgtttgccct gcgccctcaa gcgccgttga taaaaccatc
     1201 ccggctgcat ctacgatttg tactattccg acaagccgca acgggccaat cgcatacttc
     1261 atcacttctt cgtcatccgt aaatacacgc acgacccatt ccggtgcgac gagaaataga
     1321 atgccaacgc ttcccataaa tataataccg agtttcatcg cttcgtaacc tgcacgttcg
     1381 gcgcgttccg tgttaccggc gccaagactg tgaccgatca tcgtcgcggc ggcaatcccg
     1441 aaaccatatc ccggcataaa tgaaaccgat tgaatattga tcaccaaatt aactacagcg
     1501 gcttgcgcga caccgacccg atccaccata gcataaaaca ccaacaatcc gccgatgacc
     1561 atcacgccac gaaacatatt gggccaagcc agccgggcaa tctctccgac caattcccag
     1621 cgccactttg caccggaaaa taattcataa gcggctaatg tttttttacg atgccaaaac
     1681 gtgtataaaa taagaaaaga aaacccgcat atcatggaaa gcgccgagcc gacggctgca
     1741 ccggatacgc ccattgcagg cgcaccccaa tggccataga taaacatata attgccgcac
     1801 aaattggccg taaataatat gcccgccgac ataagaaata cccgtgtgcg accgataccg
     1861 ctgaaaaaac cgcgcaatgc aaagaacatc cccgacaacc aaagcgtcgc aaataaaaca
     1921 cggatgtatc ccgtcgccaa agaggctact tcttcattag ctgatatcca tcgtgtcaac
     1981 cggggcgcca tgctatacaa aacaatcatt gagaaaaaac tcaacgttag tgaaaacacc
     2041 agcgcatgat gcagtacttc gccacaggcc gggagttgcc cttcaccaaa tcggcgcgca
     2101 ctgatcgctt gcgtcgccaa ttcgacggag cggagaaccc aattgacggt ccaaaacacc
     2161 ataacggctt gaccggttgc agccaacgcc gttgcgccga gtcggcccac catgatcgta
     2221 tccgtcagac ctatcgccgt aatggatgca agccctgcaa ccaccggaag tgccagatgc
     2281 agtacctctg tagaaagttt agaatctaat gtgaaccact ttttcatacc gtgtataatc
     2341 cgtattaatc cgataacaaa atcgtatcaa gatttcatca ctatacgatg aaaatctctt
     2401 ttgttcaatc tcgcacacgc aatcttgatc actaagtttg tctaagtgat cgtataaaag
     2461 ttgatccatc cttcgtattc taatattaaa aaatcagaac ccgtcctctg acattagatt
     2521 caatgaactt catatacaat ccttgcgtga taaaattaaa caaataaaaa agccgctaat
     2581 ggaacattcc aataacggct taaccctgtc tttttgagag aaaagacgtt tatatttttt
     2641 acatctttcg ttgacgagcg gcattacgca aaaaagccgg tatagcgctg tcatcctgtg
     2701 catccgtacc ggtcttggac agaaaatcgt cttcttccgc aacgggttcg ctctctttca
     2761 tacgttcgta tgccggctga tccagtttac cgtatttctg ctctttgagt tctttcaaat
     2821 gctcataata atcatgcgga ttttttttcg tccgcaatgt catcggttgc tgcgcctgtt
     2881 cagaagccga agtcggcatc tccggggcaa cggatttggt ataacgatgt ttattgagac
     2941 cggcggcgat aaccgtgacg tgtacttcgt ttttgagatt ttcgtccacc gaataacccc
     3001 agatcacatc cgcttcttcg ccagcgacac cggtgatcat attggaaatc tgatctactt
     3061 cttccgttgt aaaatcctga gggcaggtca gattgagcaa tacgccggtt gcaccttcga
     3121 tactcgtttc ttccaggaga gggctattga tggcttgttt gaccgccatt tctccacgac
     3181 cctcgccgga accgacaccc atgcccatga tcgcatcgcc agcgccgctc atgacgcgct
     3241 tgacatcggc aaaatccaca ttgacataac cggtattgga aatcaaatcg gcaatacctt
     3301 tggtcgcacg atacagtacg tcgtcagcgg tacgaaatgc ctgaatgaac ggtgtatttt
     3361 tgggcatgac catcagaatt ttttcgttgg ggattacgat cagcgtatcc acgtttttgc
     3421 ggagttcttc aacgccgcgc aacgcctgac gcatacgttt ctctttttca aagttaaacg
     3481 gttttgtcac gataccgatc gtcagcgcgc ccatactttt agcgactgaa gccacaatcg
     3541 gtgcggcgcc ggtacccgta ccgccaccca tgccgcacgt aataaatacg aggtccgtgc
     3601 cgtctaaaat cgctttgatc tgatccatgt cttcttcggc ggcccgtttg ccgagctccg
     3661 gatctgcacc ggcaccaaga ccgcgtgtca gattttttcc gatctggatt ttgtggtgcg
     3721 cttgattgcg ctccaaggcc tgcacatcgg tattgatcgc gacaaattcg acgccggaca
     3781 gcgtggacgc gatcatcgta ttgacggcat tacatccgcc gccaccgatg ccgacgatgc
     3841 gcattttggc accgtattcg cgggcttctt cataagtaat cataataatc ctccatggga
     3901 gaggttggtg aaatgagtat tatattaatt tttaaaaaaa ttcttccgtc cagcgtttca
     3961 tctttttgga aattttctga tacaagggcg cctgatattc gccggtttgg ctctgcattt
     4021 ccaaagcgtc cgtatgcgtt tcacccggat cgccctccgt cggaccttcc ggaggtgttt
     4081 cgtcaccttc gtcttccata tcgtcatcgc ccgatttttc tttttggata ccgtagtgaa
     4141 tcaatccgat acctgtcgca tagatcgggc tattggcggc atccatgagt ccgccgaaac
     4201 cgcgcggtga accgatacgt accggcaact ggaaaatttc cgttgccaat tccgccgacc
     4261 cttccagcag cgaaccgccg cccgtgagta caacaccggc attcatcata ctgcgcaact
     4321 tacttttttc gataacggag cgcaccagat taaaaatttc catcatgcgc gcttcgatga
     4381 ctttcgagag aaactggcga ttttcacgcc gcgcatcgcg cccaccgact cccggtatca
     4441 gaatactttc cgtttcatcc acgagcgaag ccattgcgca cccgtgtttc ttctttattt
     4501 cttccgcttt gtcatagggt atttttaaac aaattgacac atcattggtc acggcttgtc
     4561 cggcaatacc gagcatatcc gtattacgaa tgctgccgtc aaaaaacatc gcgatatccg
     4621 tcgtaccgcc gccgatatca atcagtacga ctcccaactc ttgctcatcc gtttcgagta
     4681 cggcgtaact tgacgccagc ggttctaaca ctaaatcttt gactttatat cccgcgcgtt
     4741 cgacacaacg ataaatattt tgcgccgaag cgacggcgcc cgtcacgaca tgcgcttcca
     4801 cttcaaggcg cgtaccggta agaccgatcg gattttgaat accttcttgt ttatcaacga
     4861 taaattcttg cgggatgacg tgaatgattt cacgatccat cggcatattg atggctttgg
     4921 cggcatcaat cacgcgacgc acatcgtctt cggtgatctc gcgatcgtcg cgacctaccg
     4981 ctacgacagc gtggctattg attccgcgaa cgtgatctcc ggctatgccg acgtacaccg
     5041 attccgcctg aataccggcc atgagttcgg cctgctgcat ggcttttttg atggattcga
     5101 cagtatgttc gatattgacc accataccgc gccgaagtcc gaccgacggg catgtaccga
     5161 cacctttgat tttaatttct tttccgccga cctcggcgat cagcgccgcg attttggtgg
     5221 taccgatatc gagaccaacc acaatgcgat ttttcataat atcacctatg ttttatctat
     5281 gctaattcct aagaaaaatt tgcccgttgt atcgcagatc cacttcgcgc gtacccttga
     5341 ccgcgtcatc ttcttgtttg aagtgctgcg ccgccgctga aagataaacg gccgcacgca
     5401 taagttcttc gtcctctatg cgaaaagcga catgcagtga attcaaaaat accgtcatgt
     5461 cttttttatt gacgaccact tccgatatat cgtaaaacaa agattcatcg gcctctttag
     5521 cctttgcaag aaattgtaac gccagcggca agcggtcatt tttaaatttt ttgcccggtt
     5581 gcgccacgcc ggtcataccg gaaatcacag gaagattgta aatcatccgc gctttcaatt
     5641 tgggcaacac aaatccgttc gcatctacag gatacaattc gtccatcggc aaaaaagcaa
     5701 acggctcgcg ttctttgacc gtgatcacga cgctggacgg atagttacgg cttaccgtca
     5761 catgctcaaa aagcggattc attttgatgc gatctgcggt tttggtcaga tcgatttccg
     5821 ccaaacgccg tcccatatcc aaatccgccg ccttgataat atcttcttta ctgccgatca
     5881 gattgccttg cacgtacaca ttgcgtaacg agaaaagtga cgaattaacc acccattcct
     5941 gccagttaaa aagcaccata acaaaaaaca gcagcaacaa caccgaaacc gttttgacgg
     6001 caagtttatt gattcgacgc gcgcgccgct cttgttttcg ggcacgtttg gcttcctcgg
     6061 ctttgatgcg cgcttcttcc gtcagataaa aaccttccga aaaatcacga ttgatgatct
     6121 tcggtttggc ctctttggga ataattccgg gtttggtgtg aacttcaacc gcttcttggg
     6181 attcatcctc agaattttcc gacagcgtga ccgcctgcgg ttgaggttct tcagcggcaa
     6241 tggctataat ctcatcgtac gatccggaat tttctttcac ctcttccgct gccgccggga
     6301 aggcatcggc ttccgcttgt tcgctccgac caaacacttt atccaggctg atctcttccg
     6361 tcggttggct aaccgatgca cgaatggata tttcttccgc tgtcacttca tcgcgtttgt
     6421 gaatttcgcg caccgcgttt tcagtagact cgtctttttt atgttcggca gttccgttaa
     6481 attcgcccat cagaatcgga tttttaaacg tccgctcaat ttgaatcttc ttgatcacgg
     6541 tttctttgtt ttcggtgtgt cctgtttcgg ccgatttaga caaaaacgaa ggttcctccg
     6601 gaaggatatt ttccttcaca aacaaatcga cctgtgccac ttcgcgtttt tttcgtatca
     6661 tagtttaggt acgtttgcgc atcgctgcgc cgttaatccg taatcccgtc taattcttcg
     6721 tgattaaatc cgacaaactt aacttccgtt tccagacgaa taccattgcg ctgtgcaatc
     6781 gtttgtcgaa tgtgtttaat aatttcgaga acatcggcag ctttcgcttt gccctcattg
     6841 actataaaat tggcatgttt ttccgataca cttgccccgc cgattcgaaa ccctttgagc
     6901 ccggcttctt cgatcaatcg cgccgcatga tgacccgccg ggtttttaaa aacactgccg
     6961 ctgctgggca aattgatcgg ctgcgtcagg cgccttttct ccatatatgc ttttcgttgt
     7021 tcagccaacg cttctttatc accatactcc agaaccatct tacccgatac gatcaccgtg
     7081 tccggttttt ccaatgtctc cacatgacgg tacgcgaatc gaatttccga tttactgcgc
     7141 gtgactaaaa catcatcttc gagagtagtt acctccgtca gtacgtcaaa catttcttta
     7201 ccatggcaac ccgcattcat tcgtatcgcg ccaccgacag ttccgggaat ccctgcaaac
     7261 atttcgattc cagcgagccc ctgacgttca cactccagca ccagcttagg catatataca
     7321 cccgcgccgg cgcttacctg cgttttttca aattgtgtaa aagaacatgc tttttcgaga
     7381 tctatcacga tccctgaaat accgcgatcg ctgactaaca gattactgcc tttgccatgc
     7441 acaaaaaaac cgatttcgtt ttttttgcac atgcgcagca aatatttgag atcttctata
     7501 tcatccggtt cgcaccatgc tatacatggt ccaccgattt tgtaccaagt tttatccgag
     7561 agtaaaacgt tttccgtcca gcgtccgcga aatccggaac gtatatcctc tgtcgttttc
     7621 atacaccgcg caccatctga cgcccaatgt gcgtgatgtc acccgcaccc atcgtgatca
     7681 cgatatcacc cggccttgcc gcgttacgaa tggcctctga tagtttggtt ttatcgccga
     7741 tgaacgtgac atgcgcatga ttttttttcg ttgtttcgta caatttctct ccggtgatgc
     7801 ccggtatcgg catttcacgc gccggataaa tttccgtcag aatcacttcg ttggctgccg
     7861 taaatgccga cgcaaaatct tcaaaataat cccgtgtgcg cgaaaaaaga tgcggttgaa
     7921 acacggcgat gatttttcga ccgccggcac cgtcccgcgc accctcaagc gttgcgcgta
     7981 tttccgtcgg atgatgcgca taatcatcaa taaaaagaac cccgtctttt tccccgagcc
     8041 gctcaaatcg ccgtccagtc ccgccaaact tggccaggct ttcagcgata cgcgctacag
     8101 gtacttcgca catcaatccc gttgcaatac acgccagtgc attcttaata ttgtgcgtgc
     8161 cgctcatcgg cagcatcatg cgttcgattt tttttgattg taaaagaaca tcaaaatgta
     8221 attcacaaaa ttttgtttcg atatttgccg cgcgaagatc agccgcttcc gacaaaccga
     8281 aagtcatcgt acgtttaccg cactgcctta gcacttccac gacggccgga tcatccgcat
     8341 tgccgatcac cgcaccaaaa aacgaagtct gtttagcaaa ctgcgtaaac gcgcccgtga
     8401 tatcgtccag atcgcggtag atgtcaaggt gatccgattc gatattattg atcaacgcga
     8461 tatgcgaatg caattctaaa aacgagcgat catattcatc cgcttcaacc accacccaag
     8521 ccgaatcgga aagtttagca ttggtgccca gttgccggac tacaccgcca atgaacatgg
     8581 ttgggtccaa tcctgcatgc tccagcatca tgccgatcat ggcgctggtc gtcgttttac
     8641 cgtgcgttcc ggctaccgcg actccttttt ttccgcgaag aaattcaccc aacagcgccg
     8701 accttttgat catcggaatg tgctgacgcc tggcttcgat gatctcggga ttatcttcat
     8761 gcacggccgc cgtgtatact acaagatcgg cgtgcacttg ttccgcacga tgcccttcat
     8821 aacacgtaac gcctaatttc tgaaggtgcg ccgtttgttc attgaaggcc ttatccgatc
     8881 cgctcacttt ataaccctga cggaatacca cttcggcaag tccgctcatg ccgatgcctc
     8941 cgatgccgat gaagtgaata tgttgtatcg gtcgaatcac gcacgaactc cttctagact
     9001 aaacatgtgc cgtacaatgt tttgggccgc atccggttgg cgcagtgcat aactggcttc
     9061 agccatcgca cggcgatatg cttgatcttg catcatcttt cgcaacgccg gtaaaagttg
     9121 ttcattcaga tcggtttgca ataacgtcat cgccgcgccg cgatccgata aggagcgcgc
     9181 attatattcc tgatgattgt ccgccgcaaa aggatacggc acaagtacgg ccggcaaacc
     9241 catcatagtg atctctgata acgtcatggc acccgcccgg cataaaactg tatccgctgc
     9301 cgaatacgcg gcgatcatat tgtcaatgaa agcatgtacc tgcacttgcg aataaggctt
     9361 catcgtatac cgtacatttt cgtaatccgg ttttcccgtt tgccaaagaa cctgtatgtt
     9421 atcgtcttgc aataacgtat ccatgatttt aagtaatgcc gcatttaccg cacgggcgcc
     9481 gaggctcccg cccagtacaa gcaatgtgtt tctttcgctg gccaatccca acgccgcacg
     9541 ggcagccgcc atatcctgac ggacaaatcc tttgcgcaaa gggttaccgc tcatcataag
     9601 tttgcacttg ggatcaaaaa accgttgcgc atcttcgtac gtgagatgaa tcgcatcagc
     9661 ccaacgcgca agactccgtg tcgttcgacc gggacggctg ttttgttcct gcaaaattat
     9721 ttttttacca agcagcgccg ccaaaagcac gatcggcccg gtcacgtaac cgccgcatcc
     9781 gatcacggca tgcggacgaa acgtccacat cacggttatt gattgaagca gcccgaataa
     9841 cactttgaac ggcattacca gatttctaag aacatcgcgc ggatgcaaac tgcgttgcac
     9901 gccgctcatt actatcgttt tcaatctaaa tcccgcagcc ggaatgacac gctcttccaa
     9961 gccgcgattc gtaccgataa acaaaatttc cgtctccggt ttttgcgccc ggatttcttc
    10021 accgatggcc agcgctggaa atacgtgtcc gccggtacca ccggccgcta tgatcacacg
    10081 ccacgccatc aggacatgtc cataccgacg atattggtgc gaacggcttt tttcgccggc
    10141 gcttttttcg tcggtaccgg cgtcgtatcg gtcggatatt cttcctcttc gccgatttca
    10201 ccgccgacat taaaattttc cgtgaatcgg cttcgcatcg catattccgg cgccgttgct
    10261 ttcatcgctt catctttgct caccgtttgc gaagaaatat ttaacaatac acccacgaga
    10321 aaacaggtga agagcaacga ggtaccgccg tagctgataa agggcatcgg taagcccgtg
    10381 gtcggaaata cacctaccgc cacacccgca ttgaccagtg catacacgac aatgctgatg
    10441 ataatacctg tcgctaaata aaagccgtac atatcgggcg ctgtgcgcgc agttttgatc
    10501 gcacggaaaa gaaaaacaat aaataatccg agtaccagta cggcgccgat gaagcccatt
    10561 tcttcgccga tgatcgaata aataaaatcc gtaaacggtt ccggcaaaaa caacaacttc
    10621 tgctgacctt gccctacgcc gacccctgtg agcccgccat tggcaaaact gatgagcgat
    10681 tgtttgatct gataaccgct accctgcata tcagccgtcg gatcaagaaa agccatcaat
    10741 cgtgcgcggc gatacgggct gagtatgacc gtcagcaacg caaccggaat cgccacggcc
    10801 gccatcattc ccaagtgttt gatatccatg cccccgataa aaagcatcag gatgaccata
    10861 cccattacga cggcagccgt actgaaattg ggttgaagaa taatcgcaac ggcaaataca
    10921 ccggccatga tgagcggcgg taaaagaccg ttttgaaaat cacgaatgcg gtcacctttg
    10981 ctgtccatat aacctgcgac ataaaacacc agtgcgattt tggctatttc cgaaggttga
    11041 ataccgaacc ctccgacttg caaccatcgg gcggctcctt tgagttgacc gagcgtgagc
    11101 accacggcgc aaagcacgcc cgcgatgaga agcatcatca tcgtgtgccg tttgtaatta
    11161 tgataatcgt atttggcggc aaacatcagg atcacgattc ccatcattgc acgcaaaaac
    11221 tgattgacca gaaaatgcgt actgttgccg aatttttgtt cagcaatcac attgctcgca
    11281 ctgtacacca tcacgatacc aaacagcatc agcgtcagca cgatcagaaa aagccctttg
    11341 tcaatggttt tgcggggcgt taatgtttct gtagtcataa tttatttcaa accttccacg
    11401 gccattttaa actgacggcc gcgatcttca taatttttga acatatcata actggcacag
    11461 gccggcgaaa gtaaaacaac atcaccgggc gtggccgtct gttttgaaat ttccaccgct
    11521 tcttgcatgg atgcggcgcg ctgcacgcgt gtcacagcgc cgagatcacc ggcaataatg
    11581 tccgccgctt gcccgatcag tacgaggctc ttcacccgcg cttccacgag cgttttcaaa
    11641 ggcgtgtagg gcgaaccttt atgttttccg ccggcaatca aaattacttt ttctttctta
    11701 aaactatcca atgcgacaaa ggttgaatcc acgttcgtag ctttggaatc attgtaataa
    11761 cgcacacctg atttttccgc gacaaattcc aaacgatgtt ctacaccgcg aaatgatcgc
    11821 agtgtttcgg ccatcaccga cgtctttaca tcaagtgctt ttgcggccag aatggctgcc
    11881 atggcattgt acagattatg ccggccgata ataccgatat cgccggtggc gattaccggt
    11941 tcaacttttc cgtttatttt caccattaac gtatcatttt ctacgtacgc gccgttttcc
    12001 ggaatacttt gtaacgaaaa cgcccagcgc tgagccttcg aatcgccgat aagctgcatg
    12061 agcgtcgcat catcctgatt aaccaccagt acatccgacg ccgtttgatt tttgatcata
    12121 ttttttttgg ccgcataata agcctctaca ccgctataac gatccaaatg atcgggcgac
    12181 aaattgagca tgatgccgac ttgcggatga aaggattcaa atccttcggc ttgaaagctg
    12241 ctcacttcta caacggctac accgtctttg ggcaatgtct ccgtgcaatc cgaaagcggc
    12301 gtgccgatat tacctgcgac aacggtcgta cgaccggcgt gtttaaaaag ctcaccgatc
    12361 aatgtcgtcg tcgtcgtttt gccgttactg cctgtgatcg cgacgaccgg tgctgggatc
    12421 caccacgatg caatttccac ttcgctatac accggtatat ttcgcgcatg cagtgtttgt
    12481 agaacgggga tgtcggatgg cacgcccggg ctcacgaccg cccaatcgca atccgtcgca
    12541 cgttcggtgt ggccgccaaa ctcacacgcg atatgttcgt tttgtaaaag aactttggct
    12601 tcgtttttct tttcctccga agcgaattcg ctgacaaaaa ccgatgcgcc tttgtgctta
    12661 agcaagcggg cggcggcaat accgctggac gccgcgccga tgaccgttat tttttttcct
    12721 ttcacatccc gcatacgctc aacgcacttt aaatgtcgtc aacgtgagca gggcacataa
    12781 aatagccatg atccaaaatc gcgtcaccac tttagtttca tgccaacctt tgagttcaaa
    12841 atgatggtgt aacggcgcca tcaaaaaaat ccggcgccct tcgccgtatt ttttcttcgt
    12901 gtatttgaag tacatcacct gcgcaatgac ggaaatattt tccgcgacca acaccgcgcc
    12961 gatgatcggc aaaagcaatt cttttttgat caaaacagcc aacgtaccga gcgcactgcc
    13021 gatggccaaa gcgccggtat cgcccataaa tacctgagcg ggataggcgt tgtaccaaag
    13081 gaaacccaaa cccgcgccca ccatggccgt acagaaaatc gtaagttcgc tcgcttgcgg
    13141 aatgtacagg atattgagat agtcgctgaa gttgacattg cccgtcacat agcacatcac
    13201 cgcaaaggcc gtcatcgcga tcgaaacgac gccgatcgaa aggccgtcca acccgtccgt
    13261 aagattggtg gcgttggatg tcgccgtaat tacaaatatc accatcggta tataaaaata
    13321 cccaaaatca aaattgagat ctttaacaaa aggtatcgtt gtcaccgttg caaaacgctc
    13381 aaactccggt gtcatgatca ttaccaaacc gacgatcaat ccgagtgaaa tctgacccgc
    13441 cgttttatag cgcgcgacaa gacctttttt cattttttta atcgctttga gataatcatc
    13501 aataaagcct accacaccca tccacgccgt tgccagcaat acaagtaata catacgtatt
    13561 ggacaagtcc gcccaaagca tcgtcggtat caatgtggat gctaaaatga tcagacctcc
    13621 catcgtcggc gtgccggctt tggataaatg cgactgcggt ccgtcgagac gaatttcctc
    13681 gccaatctgc atttcacgca gtttgcgaat aataaacggt ccgacaaaaa gactgatgac
    13741 caacgccgta accgccgcca tcgccgaacg aaacgtgatg taacggaaaa gattggaacc
    13801 gctgatcccg gaatccgcaa atatttgata taaaaaataa aacataaaat tttttcgcta
    13861 tgctaaaatt gcctgtacaa tatcttccat tttcataccg cgcgaacctt ttactaaaac
    13921 cacatcgccg ggttgcacgg catctttcaa catctctatc aatcgggctt tatcttcaaa
    13981 ataaaacttg ttcttcatac cttgtgcgcc gtccattgca tggacggcca acggacccgt
    14041 gagatacaac gcatccacct ttttttctgc ggcatatgcg cccatgtctc tgtgctcgtt
    14101 atccgccatc gccccaagtt ccaacatgtc cgccaatacc gcgattttac gcccggacgt
    14161 ttttatactc atcaatgtgt ccattgccgc acgcatggaa ttgggattcg cattataggt
    14221 atcatccatc acggtaatcc cgtttttctc tttaaaactt acacggttcg ccggttgacg
    14281 atacgcttct aaagcctgta ctattgcttc atccggcatc ggcatgtgtt tggccaccgc
    14341 taccgctgca agcacgttat aggcatgatg caaacccggt acgccgagcc gaataagcat
    14401 cgccggtgat ttgtctgctt cgatacgcag cgagatgcac cctttatcgc tgacctcaag
    14461 tatcgttcca cgaaactgcg ctgattgatt cacgccgtat gtaacgagat cgccggccaa
    14521 ttcagtttga tcgcgtaaaa aggggtcgtc cgtatttaca aatcgtacgc catgattgat
    14581 caaatgcctg tacaatgccg ttttcgtttc cgctacggct tcgacgcggc caaagcgttc
    14641 gatgtgcgct ttaccgatat tagtgacaat gccgaaatcg ggttttatga tacgacacag
    14701 cgtttcgata tcacccgggc gatccgcacc catctcgata actgcaactt gcgtatccgc
    14761 ttccaatttc aaaagtgtca acggaacgcc gatttcatta ttcaggttac cttcggtggc
    14821 ttgcgtgatg aatgttttgc tcatcacggc tttgagcatt tctttggttg tggtttttcc
    14881 gttggaacct gtaacgccga tcactttggc acgggacttt tggcgatggt tttcggccag
    14941 tcgttgcaat gcatgcagcg tgtcttgaac gatcactaac ggcaactttt cccaagccga
    15001 cgcataacgc gcaaaagcct ccgccgtcac gacgcatacg ataccacgcg ccgcgacatc
    15061 atggacaaat gcgtgcgcat caaagcgggc accttgtaat gcaaagaaca tttgggatgt
    15121 ttgtgcatcc cggctatccg tcgaaacgcc cgatgccgac cacgtgtcta aaatatttcg
    15181 gcattcgccg atggctttca gatccgccgg tgtgaatacg ggcataacga attactgatc
    15241 ctttttggga aacagtttat aattggtttc gtcaaaatat tctttggaat gcacatccag
    15301 tttgtcggga ttattgcgaa tatccgacag caactcgttg tgtgaaaaaa caccgggctc
    15361 aaacgtctta ggatcgaaat ggcgaaatcc caattcttct tttttctttt caaaatccat
    15421 acgcctctcc cttcacttag aggttgggtt tgcactgaat gatgaccgat tcaccgtttt
    15481 tggcgacggc gccggcacgc gggtattgag cgattacgta accgttgcct tttattttaa
    15541 catcgattcc cgactcgtat aacttattca tcgccatacg catactcagg cctcgcacgt
    15601 cggggattcg tttcttgcga aagtccagcg tatccgattc actgaccgta acggcatttc
    15661 cggtcgtcaa caaaagaatg ttctgtttca atttcgggtc gtatgtccat tcttgtgtcg
    15721 tcaccatatc gccggttccc gcgatacgaa actcgagatt cattcgatcc aacacatttt
    15781 tggctgcagc gatattgaga tatttcacat ccggcgccgt catcgcggcg tcaccggcgg
    15841 tcgtatcctg atcaagatcg gccaaaacac gattgacgtg tttggcaaaa tcatccgacg
    15901 aattgataat tttttcgaga atctgtctag ttgcattcgc tgcggaggct tcaccccaaa
    15961 tgcttcggcg cgggttgtcc accgttacga taaacgcaac ctgcggattt tgtacgggaa
    16021 aaaatcccgc aaacgaagcc acataggaat tcggcgcata acgtccggta ttcgcatcta
    16081 ttttttgggc cgttccggtt tttccggcca cttcgatacc tttgatcgcc gcccgatgac
    16141 cggttccacc ctcgacgact tctttgagca tccggctgac tcgctgcatg gtctcttgcg
    16201 aagccaccgt acggatcaac tgtgtttcgt gttcggtttg tgtccgctcc agcggatcat
    16261 gcacaccttt gatgatataa ggtttgtaca gcgtaccgcc gttggctatg gcgctgtacg
    16321 ccgtcaccat ctgcagcggc gtcaccccga tttcctgacc gatggaaatc atcggacgcg
    16381 aaagacccga ccaagtgctg ggatgactca gcaatccgcg cgtctcgcct tcgagatcta
    16441 cgccggtttc gttgccaaat ccgaacgcac gcacatattg ataaagtttg tccgaaccga
    16501 tcaggtcggt cgttttgata atgccgatat tgctggattt ctgaacgacc tcggtaaatg
    16561 aaatcgtgcc gtgctttttg gtgtcgtgta tgacatgtcc atacaatgtc attttccccc
    16621 attcgcagtt tatccggtct tccggatttc gcactttttc ttccaacgcc gctactgcag
    16681 aaaaggcttt gaatgccgac ccgggttcat acaaatccgt aatggcgcga ttgcgcagca
    16741 actcggcatc aaacgtttcc ggagcattgg gatccaatcc gggataattg accattgcaa
    16801 gaatttcgcc tgtttgcgga gacaacagga cagccgtcgc attatcggcg ttgtagtctt
    16861 tgacggtttt ttcgagaacc tgataggcag ccatttgata attcatatcc accgtcagca
    16921 ccacatcttt accgttgacg ggatcttcgt gcgggtaatc gggattggga tacaaacgcc
    16981 cgagagcatc ggcctgcaag atcgcccaac catctttccc accgagcgtg gcctgctgat
    17041 ccagctcgat accggccgaa ccttcgttat caatattggt catgccgaga agcgccgcgc
    17101 ccgctttacc aaacggatag tggcgccgca gagattgcaa tttgatcaac cctttgactg
    17161 aatcattgac tttttcagct acctgatcgt tgacgcgccg ctccagccaa acaaacgcgc
    17221 cgggtttatt gagtcgtttt aaatattcgt tcttcggttt accgaaaacg cgggagaaat
    17281 tttccgcaac gcgcgctttg tcctgttcat tcataaaact cggatcggcg gcgaaggaat
    17341 agttgatcag attgacggcg attttctgca tgttgcggtc atagatcaca ccgcgttcgg
    17401 cttccaacac cgccctccgc tcatactgcc gcttggccat cgcctcgtag tcgccgatat
    17461 tggccacctg tacaaaaaat aaacgtccca cgatgaccag tgcaaacgcc actaagccaa
    17521 atgccaaaat acgcagacgt tctacgcgcg tttttacaaa agacgcgtga tctgttttag
    17581 gctggatttt aggttcgggt ttaggggtca tatttttttg cttcggcatc ctgaagcatc
    17641 ttttgttttt ctgtatctaa gaaaatcaca ccgggttttt ctgttgccgg tttcattttc
    17701 agttgctctt tggcgatttt ggcaatgcgt tcataattag acaaccgcgt gatttcggct
    17761 tgtaaactct cattagcttt ttgcgtctca agctgtttgt ctttgagttg ttccagcatg
    17821 agcatttcct gacgtaccgt cacggacatc catacccaca ctaacatgag tatagccact
    17881 acgacgaaag cgagaaacat ccggagcggt gtgatacctt ttttgttgga tgcctcgacc
    17941 gccgccgacg ctacagtttc ttgtacggcc ggtttggctt caacgcgcac ggtttcgttt
    18001 tcacgcgccg acgattttac cggcgctttg gaataccgcg cgtaacggtc ttccgttttc
    18061 gtgcgctccg ccggcgaaac cggtgtttcc gtcgtttttt tctttcggcc ggaaaaatcc
    18121 acgccgacaa tatttccact gtatgttgac atgatatctt atttcaaaaa ttatttttct
    18181 atatccgctc cgccacacgc agtttgacac tgcgcgcacg cgggttcagt tgtatttctt
    18241 cgtccgtcgc ttcataaggt ttttttgtaa tgactttcag tcgcggcgtt ttgggttcgg
    18301 acagttcggg gtagtgcgta ttgagtattt tatccatcgc ttcatctctg aaaaaatctt
    18361 tgacgatacg atcttctaac gaatggtaac tcatcaccac gatacgtccg ccgatggcga
    18421 gtatccccgt cgcatcggct aacgcctgac gcagattatt cagttcgtca ttgacctcga
    18481 tacggatcgc ctgaaaaatg cgtgacagcg ttttattggc gtgtcgctcc ggtatgatgc
    18541 gtcgtatcaa gtcggctaaa tctttggtcg tctgaagcgg ggtcgtcgtt cgctgttgta
    18601 cgatcgtacg cgcgatacgc cgggattgct gctcttcgcc gtaatgaaaa aaaatacggg
    18661 cgagctcttg ttcagaatat ccgttgacta cgtcgcgcgc cgtgaatgcg gtgtcatcgg
    18721 ccatagccat cgtgagcggc gcttcgaacc gatagctgaa tcctttgtcg gaccaatcca
    18781 gctgatgcga agaaacgcca aggtcaagca ggatgccatt gatctgtgtg ataccccgag
    18841 ccgctaacgc cgaacgcaca tgacgaaaat tatcattgat gagcgtcgcg cgatcaccgt
    18901 attccgcaag gcggataccc gccgctttta tggcatcgcg gtccatgtca aatgcaaaca
    18961 aacgacagga atcgttttgc tgaagaataa aagcgctgtg ccctccgccg cccagtgtac
    19021 cgtcaacgta gaacccgtcg ggacgaatct gaagaaaatc aatacaggat tgtaaaagaa
    19081 caggtatgtg ataatccggc atggattaaa actttacaaa tttcgcggcg gtcgtttcga
    19141 gatcaaaccc gttgtgcgcc gcatcatact gtttgggatt ccacgcttcc atacgttcga
    19201 gcgcgccgat gatgacgatt tccttttcga tccctgcaaa tgaaagtagc ggttggggaa
    19261 tgatcacacg gccttgttta tcaagttcgc actcttgtgc aaaacccatc aacaaacgaa
    19321 cgagttttcg cgcgtcggaa tccatgttgg ataattgacg taactcatcc tcacggcgca
    19381 accattccga atacggatac atgtaaatac actgctcaaa cccgcgtgta aggatcaccg
    19441 tgtctttatc ctcgggaaca aacgcgctac gcatcttcgc cggaatgttg aagcggtttt
    19501 tagaatccaa tatgtggttg tacgtgcctt taaacgaagg catacacttt aaaccccgtt
    19561 attccttaat aaaccttttt aacccactat aacccacttt cctactaaat tcaactaaaa
    19621 caagctcaat gtcaagcgaa tttttgttat ttcttacact ccggatattg acgcttcaca
    19681 aaaaatgtga cctgcgatac agacattaag agtggaattt ctttcatttc aaggttccat
    19741 gatcagacct gtttaaaagg gtatacttca gaaaaatcaa atgatgataa agttccgctg
    19801 attacatatc gcttgttgtg tgttttaaaa gcaaaaaaac gtatagagat attcaaattt
    19861 tttacaaact attttcagtt attacgctct taaatagaga tcagcacaca tcaaaattag
    19921 atatatgatt tgttgacatg ctagctctgt tgaagtatat tttagggtaa tttctcctca
    19981 tgaaaattcg taataaattc attcttactt ataaacaaac cggagtcaca cgatgaaacg
    20041 gttcatgtac gattttctcc agaaaaacgc ggcgatgtta tgcgttttat tgtgctgtag
    20101 cgcgagtctt ttcgctcaga acaatgcttt gacactcaac ggtacgtcgc agtatgctta
    20161 ctataacgga tcgttatcta cttacgaaga cgttaccatg gaaggttggt tcaagtggga
    20221 cgggagcacc ggaaacaatc agttgctgat gtacgacgga cacacttcgc tcaacggctt
    20281 tgggcttatg cttgatgcat ctgccagcaa caatctgatc attcttgccg gtggttatca
    20341 ggtcacttcg gtgggtcgcg gattagatgt cggcgaatgg tcgcacgtcg ccctcgttaa
    20401 gaaagggaac ttttttagtc tttatatcaa tggcgacccg gagatcgcca aggctatgac
    20461 ttactcatat ccttccggct cctatatggt cggcggcaac tcgatcaata attcggaatt
    20521 ttttaaaggc gccattgacg aagtacacct gtggaataga gcgttatctt acaatgaaat
    20581 ttttacggcg gcagccacgc ccatgaccgg ggcggagaca aacttagtag cgtcctaccg
    20641 tttagacaat agcgccgatg gtttaacggg cgatcaaaca actaacggtt ataatctgac
    20701 tttagttggc acacccacat ttaccgcttc gtcttcggat agcgtgatga ttcgccaaca
    20761 gacaaaatcg ctatataccg gcggcacatc ggtatccgtg gggtggcatg ccgatccgca
    20821 cagttttgtc accggctaca tcatttatgc cgatacatcc gatgccgtac ggacgcaggt
    20881 cggcactact tcatcccgca ccgatacgac gtttaccgtc acaggacttc ttcccaatac
    20941 ggtgtattat ctcacagcgc gcgccgttac cgccacggac accagcgatt attatcggtt
    21001 ggccgtggtt tccacattcg aaagcagcgg gcgtgcggcg atgttttcgg ataataacta
    21061 ctatgccgcg ccttttaatg aaaacatccc catcggaagc agcgcctaca ctatcgaagc
    21121 ttggattaaa ccgaactcca tgggcgtaca cggtattgta ggttgggggc aatatggtaa
    21181 cagttatcaa actaatgcat tgcggcttac cgaagacggt attataaatt attggtggaa
    21241 caatgatatg accatctcca ctggtaatat cgccggaagt tggcatcacg tagcggcgac
    21301 ctatgatggt acaacgcgta aaatgtattt cgacggcaaa ctcatggcct cggaaaatgt
    21361 tgtgtacggc cctgagatcc ccttttctca caacttcacc atcggcgtta ccaacacaag
    21421 cgaatacttc aacggcgaaa ttgacgaagt acgtatatgg aacgttgcac gttcggaatc
    21481 tgaaatcgtt tcattactgt tttcaccggc aacaggttcc gagagtggtc tcgcgtcagt
    21541 gtatcatttt gacgaaatcg tcggcgatta tgccggtgat tataagaatg ccaatcacgc
    21601 cgaagccgtc agcggaactc cgcaaagaac attgtcggac gcgatgttac ctttcatgcc
    21661 ggctcgttta caggcaatag ccggtgacgg ccaggtcgaa ttgcgttggg aaaaatccat
    21721 tcatccggat tttgcgagct atacattgat ggctgcaacc gatattgaat ttaccgatat
    21781 tgtagcaacg cattcctcca gcgccggcaa cgcatccgac acatcccaca ccgtagccgg
    21841 actgactaac ggaacgctgt attattttgc cgtcaagatg acaacaaact ccggcgcaac
    21901 cacaccgttt cgcatgaatg cggcagtccc taataccggc ggtcccggca atgcgcttat
    21961 atttgacggc gaagatgatc agatcatatt ggaagataac ttggtgcgat cacgctccga
    22021 aatgactatc gaaacatggt ttaaaaccac aacttccggc ggcatttttg gttataatga
    22081 cgcctcgaat gcagagcatg tccctgtaat ctatgttggc atggatggat atcttcaatc
    22141 tcaattgtgg atcggcgatt ataattctgt catgcgatca cttcgacctg tgtcagatgg
    22201 ttattggcat cacgtcgcgc ttactgccgg taatgacaat caatacctat atattgacgg
    22261 ccagctgtgt gactcaatta tcggtcgacc gctcaatcat ctaagcatga tcaaaaacca
    22321 aatcggtagg gctactactt caggttggcc aaatggcggg aatgaatact ttagcggcga
    22381 gttggacgaa tttcgttttt ggaatcgcgc cctctcacat caagaagttt attcttatgc
    22441 caacaagtct ctgcgcggag atgaaaacgg gcttgtccta ttgcatcatt ttaatgagcc
    22501 ctccggtttg atttatacgt atgacgccgg gcccaacgga tggacgggtg gattggagag
    22561 tttctccgca tcaacaacgg tggcatccgg agcgttgcga ccattctcac cgtataattt
    22621 acaatctacc aatacattaa cttccatcac cttaaaatgg aatcggagta acgccgcgtc
    22681 tgtccgggct tactacattt atacttttga cggcgaatcc acaatgcttt gggattctac
    22741 gactggcgga ttgaccgaca cgacgataac attcgccgaa ttaccgtcat tcacaaattt
    22801 cacctattac ttatcgaccg tagatgcatc gggttccgag tcggaaaaat ctaaccccat
    22861 tttcgtgacg acaacttcct tcaaagaagt taccggaacg actttctcac aagtcgttga
    22921 cggaaaattc caatgggcgg attatgataa cgatggcgat tatgacgtat tagtcgccgg
    22981 ttcggaaaat aacggctact atcctcccta tcaaaacgga acgatcaaat tgtatcgtca
    23041 ggacaacggt ggaacattta ctttagttga cgtaggccta actcaagaaa ttcatcgcgg
    23101 tacggtggct tggtgcgact atgacaatga tggacttccg gacatctcct atatgggaag
    23161 cggcggcaac ggaacacgcc ctttcaaatt atataaaaat gacggcgacg gcacgtttac
    23221 ggaagtccta acggcaatac aaggtgcgct attcggtgca atggcctggg gcgactatga
    23281 taacgacggc gatcaggacg tgtttgtcat gggttcgata ggaagcgaag gcggggataa
    23341 ctattccatg cttttcagaa atgacagaaa tgatctgttt actcaagtag acatcggtct
    23401 tatcggatat tccggaggtt cagcagcttg gggcgattat gatcgagaca acgatcttga
    23461 tcttttgatt ttaggttcaa ccaacggtag ttataccggc ggtgatc
//
LOCUS       DAOQGF010000043        23230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1011_length_23230_cov_7.651478, whole genome shotgun sequence.
ACCESSION   DAOQGF010000043 DAOQGF010000000
VERSION     DAOQGF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1011_length_23230_cov_7.651478"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1288)
                     /locus_tag="PK937_07740"
     CDS             complement(59..1288)
                     /locus_tag="PK937_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNH32499.1"
                     /translation="MRNKNLEAYYGLPNNVFFCKRCVMSNQRPTSTIEFRHIAGSKKN
                     TMNFDDKGICDACRHAEIKKTIDWKKRESELLRLLEKYRRSDGNYDCLVPGSGGKDSA
                     YQAHILKTKYGMNPLTVTWSPIMYTDYGYQNWKNWIEVGGFDNLLFKQNGKAMKLLTK
                     LSIVNLLHPFQTFILGQKNLAPKVAAKFGISLIFYGENEAEYGNPIADNVSSLRDKSY
                     YSFSNLNEVFLGGVCVPDLISNYGLTLNEIMPFLPATSDELKRCEVEVHYLGYYLKWT
                     PQEVYYYAVENTNFTARPFRTQGTYSKYNSIDDKIDDLHYYTTYIKFGIGRATYDASQ
                     EIRNDHITREEGQALVRKFDGEFPDRYFEEIMRYLDINPSDFHMLCDKFRSPHLWAQV
                     NGEWKLRHTVNSDGVDD"
     gene            complement(1292..1966)
                     /gene="hisH"
                     /locus_tag="PK937_07745"
     CDS             complement(1292..1966)
                     /gene="hisH"
                     /locus_tag="PK937_07745"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNH32500.1"
                     /translation="MKTQVSHRVSVCIIDYHLGNLFSVKRACELIGLRPIISSDPNVI
                     RNSDALILPGVGAFADAMRNLKNMKIDVAIKDHINDHKPFLGICLGLQLLFSESHEFG
                     IHQGLGVIQGKVVRFQNENRKLKVPQVGWNFIKKTGGLPDVVWGKTPLFDIDIKQRMY
                     FVHSYYVVPEDKSVQLTETEYEGVKYCSSVFTKNIFACQFHPEKSSYEGLKIYKNWAD
                     FLEHKD"
     gene            complement(1971..2816)
                     /locus_tag="PK937_07750"
     CDS             complement(1971..2816)
                     /locus_tag="PK937_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNH32501.1"
                     /translation="MNKRIIARLDIKGPNLVKGIHLEGLRVLGDPSKFAHSYFHEGAD
                     ELIYQDVVASLYDRNSLHSFIAKAAEQILIPLTVGGGIRTIEDIRTILRFGADKVSLN
                     TGVLKRPELIREAANSFGSSTIVIAIEAIRQKDGKYYCYIDNGREFSGREVADWVCEV
                     EALGAGEILLTSVDRDGTGLGFDIELVKMVSGLVNIPVIAHGGCGKIEDISEVFKVGN
                     ADAVAVASVLHYGMIGKEVRALHDITEGNFEFLKSNKSFSKITPLSMLEIKKQLIDLG
                     YDIRN"
     gene            complement(2809..3525)
                     /locus_tag="PK937_07755"
     CDS             complement(2809..3525)
                     /locus_tag="PK937_07755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNH32502.1"
                     /translation="MSYKEKSILAIIPARGGSKGVPAKNIRPLAGKPLIAYTIEAATK
                     SKYVDRVIVSTDSETIADTCNIYGISIPFIRPSEISTDESPTWQTVEHAVDWLSNNEK
                     KTYDVICLLQPTSPLRNVGHIDQSISKFLSCNADSLVSVCRSPKSPYWSHHITPDGYL
                     SPLFPDSENYIRRQDVPVTFDLNGAIYLVKTEFFRRTHLLKGPRTVAYEMDYQSSIDI
                     DTPLDFEIAEIIRKQLGSHE"
     gene            complement(3528..4595)
                     /locus_tag="PK937_07760"
     CDS             complement(3528..4595)
                     /locus_tag="PK937_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNH32503.1"
                     /translation="MKESIDQFFIDPTVTVRDAMKKLNSTGRKVLFVRTSQGTLFGSI
                     TDGDIRRWILKGGQLEAKASEICNITPHVVESNYDIEYVKSIMVRENIESLPVVTSDG
                     CVEKLLFWEEVFAKANVRRSGFSIDIPVVIMAGGKGTRMDPFTRVLPKPLIPIADKTI
                     LEFIFDEFVQFGVARFFLTLNYKGELIEAYVKSLNKSYQVDCIWEKDFFGTAGSLTLV
                     RNHIESDFILSNCDNIVKADYHEVLEFHRQNNAWLTILSSIQNHRIPYGVVSFEDGGT
                     VNRIIEKPEYSFHVNTGVYIINRKALDYVPDHTIFHMTDLIDRLLLDKRKVMTYPVTE
                     DDYIDIGQWDEYKKVVEKLKI"
     gene            complement(4598..5776)
                     /gene="neuC"
                     /locus_tag="PK937_07765"
     CDS             complement(4598..5776)
                     /gene="neuC"
                     /locus_tag="PK937_07765"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNH32504.1"
                     /translation="MKKQKRHKLCVVTGARSEYGLLRPLIFALSKSKNFDLCVLVTGM
                     HLSEGFGMTESEIESDGFEIHERVEMLLDSMSQIGVSKSIGVGMIGLSDALSRLNPDA
                     MIILGDRFEAFAAAATAHVLRIPIIHLHGGELTEGSIDDGFRHAITKMSSLHFVSTEV
                     YRDRVIQLGEHPERVFHVGAIGIESIRSLKLKSEKELCRELNLPTLGNSLIITYHPTT
                     IANGNVEKECNELFHALEKFNNMTMIFTGSNADADGQRINKLMQQFVGKKYKQRRFFQ
                     SLGQLRYLSLLRFVKGVVGNSSSGIIEAPEFGVGTVNIGSRQDGRVKPRSVVDCACKR
                     SDIEMAIRKIISPEFSTVLDALDHPYGKGKTTEAIMDVLKSTQFSKLNRKKFFDIKKH
                     "
     gene            complement(5773..6777)
                     /gene="neuB"
                     /locus_tag="PK937_07770"
     CDS             complement(5773..6777)
                     /gene="neuB"
                     /locus_tag="PK937_07770"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HNH32505.1"
                     /translation="MNKVLIIAEAGVNHNGELRLAKKMIRAAAQAGADVVKFQTFRTE
                     SLVTTKAPKAKYQKKRDNADESQFQMLKRLELPYSKLDILQKECERNRVLFLSTAFDL
                     ESLDALHKMKLPFFKIPSGEVTNYLYLKKIAQFGRPVILSTGMCTLDEVEKAIRLLEK
                     NGIRRSFITLLHCNTEYPTPYRDVHLRAMQTLRSEFGVNVGYSDHTMGTEVAIAAVAL
                     GATVIEKHFTLDCGMSGPDHKASIEPRDFKRMVDAIRHIETALGRSTKKPSASEKKNI
                     KIARKSIVASQDIKKGDFFSEKNITVKRPGTGISPMQWNRVIGRQAKKDFNLDDLISI
                     "
     gene            complement(6770..7411)
                     /locus_tag="PK937_07775"
     CDS             complement(6770..7411)
                     /locus_tag="PK937_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNH32506.1"
                     /translation="MSEKIKLVLVGGGGHCRSCIDVIETLEGVDILGVIDTDPSRSSI
                     GNYPILGDESTQNLDRLNPDYFLITVGQTKSCEKRKSIANTLKQHNKLFATIISSLAH
                     VAHNSFISQGTIVMHRVVVNANVHIGINCIINTGAIIEHDVIVGDFCHVSTGAIVNGN
                     CKIGKETFIGSGAVIKHGVSVCDCTIIGAGSVVVSDILESGTYVGVPARRLYE"
     gene            complement(7404..8564)
                     /locus_tag="PK937_07780"
     CDS             complement(7404..8564)
                     /locus_tag="PK937_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HNH32507.1"
                     /translation="MSKVFFDEVVDFIRSWYDTDQPIPLHAPVFHGNERKYVLETIDS
                     TFVSSVGEYVTKFESMVADYTGATEAVATSNGTVALQIALLVSGVRHGEEVITQPLTF
                     VATSNAIAHVGAIPIYVDCSLSTMGLSPDSLNEFLETNAINIKGESYNRKTGRRLAAC
                     IPMHTFGHVVEIENIRVICDRFNIPLIEDAAESLGSYCGDTHTGLIGDVGVLSFNGNK
                     TITTGGGGMIITKNKQFALRAKHLTTTAKRPHQWEFFHDEVGYNFRLPNLNAALGCAQ
                     MERLPDLLRDKRELAYAYRDFFKDTKVRFFEEREGTTSNYWLNSIILSDRAERDEFLS
                     FTNKKNIATRPIWTLMNKLPMYQKCQTTPLPNAEWFEDRVVNIPSSPRVKNV"
     gene            complement(8557..9531)
                     /locus_tag="PK937_07785"
     CDS             complement(8557..9531)
                     /locus_tag="PK937_07785"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNH32508.1"
                     /translation="MDKVLITGAGGFIGSHLVELCVASGFSVKAFVHYNSKNEWGWLD
                     TLEEKANLEIVSGDIRDYDSVYSALEGCVGVFHLAALIGIPYSYVSPKAYIDTNIIGT
                     YNVLQGSKCLGLDKIIITSTSETYGTAQYVPINESHPLVGQSPYSATKIGADQLAISY
                     YRSFNLPVTIVRPFNVFGPRQSARAVIPTIVSQLLSGRRSIKLGSLNPTRDLTFVKDT
                     AAGFVEAFRSQTLKGETVNIGMGFEISIGDLAKKIGKLMQVEFEFESDSVRIRPEHSE
                     VERLWCDNTKLLKNTNWSPKYNLEIGLKETISFIEKNLNIYKPEIYNV"
     gene            complement(9571..10797)
                     /locus_tag="PK937_07790"
     CDS             complement(9571..10797)
                     /locus_tag="PK937_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNH32509.1"
                     /translation="MNNSNNTNDSGIEGQAAFPKSLGDWMEKLYPYRKKIIVTSFFIT
                     VATVAFLFLTKPWYESQAVILLPDKSSSAIESLGGALGGIGQTLLSGSGISGPQRYLA
                     ILRSQRLKEAIIARFKLIDFFEMEPTRKDTSALLELMEESIFFESNVKNSTISIQVRF
                     QGSPEMTADITNFAVSELDRINRELSTDQARFSRQFIEKRYYDSRIELKKAEDSLNFF
                     QKRHGIIALTEQTKASIETAADIQAQLVTGEAEYNIIRKSVGDNHPDAVRQKSRLDEL
                     RRIQNKMDVGGFNQSILIPFNSTADIALQYLRLFREVQINQKIVEYLIPQFEQTKIQE
                     AKDTPTVQVLDYGKPAAYPVKPKKKIITIIVGFLSLVLTSLFYVVNDIIRVSDMSKQN
                     RVTAIFKAFHLKGSTK"
     gene            complement(11234..13102)
                     /gene="asnB"
                     /locus_tag="PK937_07795"
     CDS             complement(11234..13102)
                     /gene="asnB"
                     /locus_tag="PK937_07795"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNH32510.1"
                     /translation="MCGIAALINTKPTDGALIQRMTDIVSYRGPDGDGHNGFDGNRVW
                     LGQRRLAIIDLSNAGREPMSYKNERYWITYNGEVYNYIELRRELEAVGYVFASKTDTE
                     VVLAAYDYWGTECLHRFNGMWAFVLYDTLTKRTFVARDRFGVKPLYYYKTTDGGYAFA
                     SEIKQFSVLPGWRYSMNRQRVADFLGYAISDHTDETMYDRVLQLRGGECAEITYEAQT
                     VRFEKRRWYSFPDTQFDGTYEDATKQFLNVFTDAVRLRLRSDVPVGSCLSGGLDSSSI
                     VCLLHDMLSTQNTTALQKTFSAYAREQAYDESRYAEEVIRAIRVEAHHVYPDFETLFD
                     TLDTLLWHQDEPFGSASIYAQWHVFRLAKSGGVTVMLDGQGSDEQLAGYYPFYGTRLA
                     ELMVRGRWIRLISEIQKTIGEGHVNRGAVLRRLIGALIPNAWMPFMPSDSQSSNDWFD
                     MEKLGARSIHWRQQYGEFKPDVNTALRVQLTRTNLPMLLHWEDRNSMAHSIEARVPFL
                     DYRLVEFILSLPSDHKISNATTKRVLRDSMKGYVPESIIQRRDKMGFVTPEEHWVRTH
                     PDVFRKQLSSSIERSGGVIRSSLRDDFDAMIAGKKAYDGRYWRGIVFGRWVRSDSM"
     gene            complement(13127..14248)
                     /locus_tag="PK937_07800"
     CDS             complement(13127..14248)
                     /locus_tag="PK937_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNH32511.1"
                     /translation="MKNNIILSIGVPTWNRSGFLKALLESIIHQAEKFDFTRSVEIIV
                     SDNCSTDDTGVVVKAMSAQTNVAIRYVRNSENIGAIRNMIQTFHQSRGRFWMAFGDDD
                     ILAEGILPEIMHHLTTRDDFSVVMFDQWKLIGHESLMTLESAAERYFYYMGNAGVFAI
                     RTEHARQGLKPGDDALIAQSYWPQTHIAFLAMKSGNLSKPFLSLPLISSYSPNHTENT
                     LYSGWYLWETGIRSLQHVALILKPELGTSFFRAVCRHLFWTRRLFFYLKNVMLYTAFF
                     DSPRQIRLLRENVRRHMRTISMCNGLALVMFFVLIELPPSVKRVLYSFIFMMLKPRQW
                     PHAKRDILVKKAIHERAHAAENEKGVPRSYDRDVQPEAS"
     gene            complement(14248..15558)
                     /locus_tag="PK937_07805"
     CDS             complement(14248..15558)
                     /locus_tag="PK937_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNH32512.1"
                     /translation="MSAPPSSIPMTLRQLVERIKQSGYLKNVSRLSIGTIIGQAIAVL
                     LLPIVTRLYDSADYGIAATLNAMVMIASSTIMLRMDSAIVLADSDDQAAKIIRFCGQL
                     ILGFAIVVAISAVVFYGLALDLGFFPKGGPLYFWLPVWVILGAGNILLASWANRYAAY
                     RVLSFSRALPPLLAFIVMLSMYSWQGSTITGLVLGHISAAITLYITIFFGLRHEGKPI
                     RHLAVDWADIRNLLKRFKQFPTYGLLMTFLDQLTASLPLLIFTASFSPHDAACFALAT
                     NVLRLPSTVIGQGVAQVFYEMSAKLSEDATLLRSFVINNIKFLSFFSFPFLIIIVAAG
                     PWLFEWVFGAAWRDAGEYARSLVIAIAVNLVASPVSMISSVIRKQRMHFVISLLSFAI
                     RAAMIGIGVTSGSAYTTVILYSIGEAMMLIVFLIWVVRSVRMRA"
     gene            complement(15569..16471)
                     /locus_tag="PK937_07810"
     CDS             complement(15569..16471)
                     /locus_tag="PK937_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNH32513.1"
                     /translation="MRTAFIQQLMSEAKRDERVFLLVGDLGYSVVEPFAESFPDRFLN
                     IGVAEQNMAGIAAGLAKEGYIPFIYSIGNFPTLRCLEQIRYDICYHALGVKIVSVGGG
                     FAYGALGASHHGTEDLGILRTLPSMIVTAPGDPQEARLITTHAVQTDAPMYIRLGKAG
                     EPAVHPGNIAFAAGKMIPVCHGKNTVVLTTGAMLRYASDFIRHNNLLWGLYSTPYIKP
                     IDSATIELLAHSYERIITLEEHQRSAGLGSAVLEALHDCREKKKSMPIPLVHRIAIPD
                     QFIQIAGSQDYLRQSCGLVLSEYI"
     gene            complement(16471..17280)
                     /locus_tag="PK937_07815"
     CDS             complement(16471..17280)
                     /locus_tag="PK937_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNH32514.1"
                     /translation="MVTTDEVKAFSKAIRIEALKMVHASNSSHLGGAFSMADILAVLY
                     GGVMNVNPKNPRWPERDRFLLSKGHACSALYAALALRGFFDMSQLTSFAQNGSFFTSH
                     ANHHIPGVELSTGSLGHALSVGCGLALAAKRRGQAWRVFTLLSDGELDEGSNWEALLF
                     APHHHLNNMVVIVDHNKIQSLGHVNEVLDLGPLSEKFKSFGWYVTVTDGHDHAALSAA
                     ITKKTDDKPHIIIAETVKGKGVDFMEEKLLWHYRTPDAEQFRNALEQLEHS"
     gene            complement(17274..18200)
                     /locus_tag="PK937_07820"
     CDS             complement(17274..18200)
                     /locus_tag="PK937_07820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNH32515.1"
                     /translation="MKITDIQNQYRSGAIKKSEYIQAMYEKHNQLFEYTDILRHTDIE
                     NITITSHGIVWTTRPHRLRWLTPKADKRSAPFEILNFAQYEPEYFSMMCHLLEPGMSV
                     FDIGANIGWYSLNFSKAVSDLHVCAFEPVPGTYLELENNIQLNTGMRVKSYNLGLSDR
                     AGKLTFFVDPELSTSASAVNITEAANAHETECALETMDNFCKQYGAKPDFIKCDVEGA
                     ELMVYRGGKYTLQTHKPIVCTEMLRKWAAKYNYHPNDIITFFKEMGYQCFFIQNDYLA
                     PITFVDETTVETNFIFLHRERHADKIKTWVKS"
     gene            complement(18197..19240)
                     /locus_tag="PK937_07825"
     CDS             complement(18197..19240)
                     /locus_tag="PK937_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNH32516.1"
                     /translation="MHSIIREDIAHIISAPLPWDILRGKTVLISGANGFLPAYMVETL
                     LGLNDRLDMNIHVIGIVRSEERARQRFAHHAGRNDFKLHVADVTRLVFRSDTPVHFVV
                     HAASQASPKYYGIDPVGTLTANTTGTQNMLEVARQSQSERFLYFSSSEVYGQLEARQI
                     PTNEMQLGCLDSMALRSCYAEGKRLGETMCVAWSHQYGLSTVIVRPFHTYGPGMALDD
                     GRIFADLVSDIVNGKNIVLRSSGAATRSFCYLRDAIIGFFTVMLHGKTTEAYNVGNPE
                     CEISIRGLAEKLVTLFPEKKLSVVLDIPPQNGYIPSAVQRSVPDITKIKSLGWQPTTT
                     VEEGFTRTIKSYT"
     gene            complement(19240..20595)
                     /gene="rfbH"
                     /locus_tag="PK937_07830"
     CDS             complement(19240..20595)
                     /gene="rfbH"
                     /locus_tag="PK937_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNH32517.1"
                     /translation="MDQNVKAAAEKLRLEILEKTAEYYRLTHGQKKEFVPGQSAISYA
                     GRVFDEKEMQNAVDASLEFWLTSGHFSKEFESKLAKKLGVKHAMLCNSGSSANLLAFM
                     GLTSPLLEDRRIRKGDEVITVAAGFPTTIAPMLQFGAVPVFLDVHTETANIDTRALEA
                     AYSPKTKAVMIAHTLGNPFDLDAVTAFCKKYNLWLVEDNCDALGSEYRGRWTGTFGDV
                     ATSSFYPPHHITMGEGGAVYTNKSILKRSIESFRDWGRDCWCDSGKDNTCGKRFDWEL
                     GTLPCGYDHKYTYRHIGYNLKATDIQAAIGCVQLDRLEDFADRRRANWNYLKNGLAEF
                     SDIFILPEATPNTNPSWFGFLISLRSESGISRPELIHYLENKKIQTRMLFGGNLIRQP
                     FFDAFREQPGSYRVVGDLTNTDLIMNNGFWLGVYPGLTTPMLDYMIDAIRSFLRRDTV
                     S"
     gene            complement(20652..21743)
                     /gene="rfbG"
                     /locus_tag="PK937_07835"
     CDS             complement(20652..21743)
                     /gene="rfbG"
                     /locus_tag="PK937_07835"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNH32518.1"
                     /translation="MGDLVKNNFSDIYRDKRVLVTGDTGFKGSWLSVWLHTLGAEVYG
                     FALPPKSNNDNFVRCDLQNKIHHRDGDIRDPHALADYVKEVRPDIAFHLAAQALVLDS
                     YRDPAETFETNVVGTVRFFEAVRAISSVRAAINVTSDKCYQNNEWVWGYRENDPMGGA
                     DPYSASKGASELITSSYAQSFFSSEGTCSIASARAGNVIGGGDWAENRIIPDFFRAVQ
                     KNQTLILRNPNAVRPWQFVLEPLRGYLTLGEKLFLHGKTYSGGWNFGPAMHHACSVQE
                     LILALISKTGFGRFETAAVKQNEHEAYMLRLDISKAVSRLSWKPALSLEQTIALTAQG
                     YHDDAFAGSLWDARKKQLDEYLSLSSHTI"
     gene            complement(21728..22501)
                     /gene="rfbF"
                     /locus_tag="PK937_07840"
     CDS             complement(21728..22501)
                     /gene="rfbF"
                     /locus_tag="PK937_07840"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNH32519.1"
                     /translation="MKVVILAGGYGTRISEESHLKPKPMIEIGGKPILWHIMKIYSGY
                     GYNDFVICLGYKGYVIKEYFANYFLHESDVTFDFRAHNAPVIHTHTAEPWRVTLVDTG
                     LDTMTGGRIRRVASYLDGERFFLTYGDGVADVDIRKLLSFHMTHGKTATVTAIQPSGR
                     FGAMAMDSSGMVQSFQEKPKGDGAWVNAGFFIFENSVLQLLKDDQTVLEREPLETLAR
                     SQSLVAFKHDRFWQPMDTLRDKNMLDELIREKRAPWVIW"
     gene            complement(22503..>23230)
                     /locus_tag="PK937_07845"
     CDS             complement(22503..>23230)
                     /locus_tag="PK937_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNH32520.1"
                     /translation="KKHNLKVIEDGAQAIGVQYSDGKKVGNFGDIGCFSFFPSKNLGC
                     FGDGGLVTTNDDALGEKLRVLRVHGGKPKYYHKMIGGNFRLDAIQAAVLNVKLPHLDN
                     WSQRRRENADLYTQLFIAAGVAEAEGKITYDSKNKVLLPKAAYRTSGAKNHHIYNQYV
                     IRVEKRDALRKYLTDKQIGNEVYYPVPFHRQECFGYLGEKDTDFPNANAQAEQSLAVP
                     IYPELTKEQISEVVNTITEFVKA"
BASE COUNT         6730 a         5535 c         4346 g         6619 t
ORIGIN      
        1 tttttttgat acaaactcac aaaacattta catataataa attttatgaa ctgcgcattt
       61 aatcatccac tccatcacta tttacggtat gtcttaattt ccattcccca tttacttgtg
      121 cccataagtg gggtgaacga aatttatcac ataacatatg aaagtctgaa ggattaatat
      181 ctaaatacct catgatttct tcaaaataac gatctggaaa ttcaccatca aactttcgaa
      241 ccagtgcctg cccctcttcc ctcgttatgt gatcgtttct tatttcttga gaggcatcat
      301 aagttgcgcg tccaatgccg aatttaatgt atgtagtata ataatgaagg tcatcgattt
      361 tatcatcaat actattgtat ttactatatg ttccttgagt cctgaacgga cgtgcagtaa
      421 aattcgtgtt ctctacagca taataataca cttcttgcgg tgtccatttt agataatacc
      481 ccaagtaatg cacctcaact tcacaacgtt ttaattcatc ggaggttgcg ggtaaaaaag
      541 gcatgatttc attcaacgtc aacccataat tggaaatcaa atcaggaaca cacactcctc
      601 ccaaaaaaac ctcgttgagg tttgaaaaac tgtaatatga cttatctctt aacgaagaaa
      661 cattatcagc tataggattt ccatattcgg cttcattttc accataaaat atcaacgata
      721 taccaaattt agcagctacc tttggagcta aatttttttg tcccaaaata aaagtctgaa
      781 atgggtgtaa caaattcaca atgctcaact tcgtgagaag tttcatcgct ttaccgtttt
      841 gcttaaataa aaggttatca aaccctccaa cctcaatcca atttttccaa ttttgatatc
      901 catagtctgt gtacatgata ggtgaccatg ttacagttaa cggattcatc ccgtattttg
      961 ttttcaatat atgcgcctga taagcactat ctttgccgcc acttccagga acaaggcagt
     1021 catagttacc atcggaccgc cgatactttt ccagtaaacg caataattcg ctctctcgtt
     1081 ttttccaatc tattgttttt ttgatctctg catgacgaca tgcgtcacaa attcctttat
     1141 catcaaaatt catagtgttt tttttgctgc ccgcaatgtg tctaaattca attgtagaag
     1201 tcggtcgctg attcgacata acacatcgtt tgcaaaaaaa cacattattt ggcaaaccgt
     1261 aatacgcttc taaatttttg tttctcatct tttaatcctt atgctcaaga aaatctgccc
     1321 aatttttata aatttttaat ccttcataac tgcttttttc tggatgaaat tgacacgcga
     1381 aaatattttt agtaaaaacc gaggaacaat atttaacccc ttcgtattct gtttctgtca
     1441 gttgaactga tttgtcttcg ggaaccacat aatatgaatg cacaaagtac atgcgctgct
     1501 taatatcaat atcaaataat ggagtttttc cccaaacaac atcaggtaaa cctccagttt
     1561 ttttgataaa attccatcct acttgcggta cttttaattt tcggttttcg ttttgaaacc
     1621 taactacttt cccttgaatt acccccaaac cttgatgtat accaaactca tggctttctg
     1681 aaaaaagaag ctgtaaacct agacagatgc ctaagaatgg tttgtggtca ttaatgtgat
     1741 ccttaatagc aacatcaatt ttcatgtttt tgagatttct cattgcatct gcaaatgcac
     1801 cgacaccggg caaaatcaac gcgtcagaat ttctaattac gtttggatct gaagaaataa
     1861 tcggacggag cccaattaac tcacacgctc tcttcaccga aaaaagattc cccaaatgat
     1921 aatcaatgat acagacagaa actcgatgag atacttgtgt tttcattttc ctaatttctt
     1981 atatcgtacc ctaagtctat caactgtttt tttatttcta acatagataa cggggttatt
     2041 tttgaaaaac ttttattact tttcaaaaat tcaaaattcc cttctgttat gtcgtgcaag
     2101 gcacgtactt ctttacctat cattccataa tgaagcacag aagcaacggc aacagcatcc
     2161 gcatttccaa ctttaaaaac ctcagaaata tcttctatct ttccgcatcc accatgcgca
     2221 ataacaggaa tattaactaa acccgacacc atcttaacca actctatatc aaaacccaaa
     2281 cctgtaccat ctcgatcaac cgatgtcaac aatatttctc ctgccccaag tgcctcaacc
     2341 tcacaaaccc agtcagcaac ttcgcgacct gaaaactcac gaccattgtc aatataacag
     2401 taatattttc catctttttg tcttatagct tcaattgcaa taacgattgt cgatgagcca
     2461 aacgaatttg ccgcctctcg aattaattct ggtcgtttta gcactccagt atttaatgaa
     2521 accttatcgg caccgaatct gagaatggtt cttatgtctt caatagtacg gataccgcca
     2581 ccaacagtga gtggaattaa tatctgttct gcggccttag cgataaagct atgaaggcta
     2641 ttacggtcat ataaactagc aacaacatcc tgatatatta actcatctgc tccttcatgg
     2701 aaatatgaat gagcaaattt tgaaggatct cctaaaacgc ggagcccctc aagatggata
     2761 cctttgacaa ggttaggacc tttgatgtcc aatcgtgcta taattctttt attcatgcga
     2821 tcccaattgt tttcttatga tctcagctat ttcaaagtcg agtggagtgt caatgtcaat
     2881 tgaactttga taatccattt catacgcaac tgttcgcggg cctttcaaca aatgtgttcg
     2941 cctaaaaaat tccgttttta ccaaatagat cgcaccattg aggtcaaatg tcaccggcac
     3001 atcctgacgt cgaatataat tttctgaatc tggaaataat gggcttaggt aaccatccgg
     3061 tgttatatgg tgtgaccaat aggggctttt aggagatcga catactgaaa ccagtgaatc
     3121 cgcattacat gacagaaatt tggagatgga ttgatcgata tgccctacat tgcgcaaggg
     3181 agacgtgggt tgtaggagac aaatcacatc atacgttttt ttctcattgt tgctcaacca
     3241 atcgacagca tgttcgactg tttgccaagt cggagattcg tccgttgata tttcagacgg
     3301 gcgaataaat ggaatactaa taccgtagat attacaagtg tccgctatcg tttccgaatc
     3361 cgtactaaca attacacgat ctacatattt ggactttgta gccgcctcaa tagtataagc
     3421 aattaaaggc ttgccggcca acggccgaat attcttcgcc ggaacgcctt tacttccgcc
     3481 acgcgcgggt atgatagcta gaatactttt ttctttataa ctcatgatta tatttttaac
     3541 ttttctacca cttttttata ctcatcccat tgtcctatat caatataatc atcctccgtt
     3601 accggatatg tcattacttt tcgtttgtct aataaaagcc gatcaatcaa atccgtcata
     3661 tgaaaaattg tatgatcagg tacataatct agagctttgc gatttatgat atacacgccc
     3721 gtatttacgt gaaaagagta ttcaggtttt tcaattattc ggtttaccgt tcctccatcc
     3781 tcaaatgaca cgacgccgta tggaatgcga tgattctgaa tagacgataa aattgtcaac
     3841 caagcattat tctgtctgtg aaactctaag acttcatgat aatccgcttt gacgatatta
     3901 tcgcagtttg ataaaataaa atcactttcg atatgatttc gcactaacgt caagctccct
     3961 gcggtaccaa aaaaatcttt ttcccaaata caatccacct gatatgattt attcaaagac
     4021 tttacataag cctcgataag ttcaccctta taattgagtg ttaaaaaaaa acgcgccacg
     4081 ccaaactgca caaattcatc aaaaatgaac tctaaaattg ttttatcagc aataggaata
     4141 agcggtttag gtagaactcg tgtaaacgga tccatacgag taccttttcc gccagccatg
     4201 ataacaaccg gtatgtctat agaaaaaccc gaacgacgaa cattagcttt tgcaaaaact
     4261 tcttcccaaa aaagcaattt ttccacacac ccatcgctgg tgactacagg aagcgattcg
     4321 atattttctc taaccataat tgatttgaca tattcgatgt cgtaattgga ttccacgaca
     4381 tggggagtta tattgcatat ttcggatgct ttagcttcaa gctgaccacc tttgagtatc
     4441 caacgacgaa tgtcaccatc cgtaatactg ccaaagagcg ttccttgaga agttctaaca
     4501 aacaatactt tacgcccagt actgttcagt tttttcatcg catcacgcac cgttacagtt
     4561 ggatcaataa aaaattggtc tattgattct ttcattgtca gtgttttttt atatcaaaaa
     4621 acttttttct gtttaatttt gagaactgag tactcttcaa aacatccatg atggcttccg
     4681 tcgttttccc tttgccgtag ggatgatcta gtgcatccaa aacggttgaa aattcaggtg
     4741 aaataatttt cctgatagcc atctcaatat cgcttcgttt gcatgcacaa tctacaacac
     4801 ttcgcggttt tacacggccg tcttgacgtg aacctatatt cacagtacca actccaaatt
     4861 caggtgcttc aataatgccg cttgacgagt tacctacgac tccttttaca aatcgaagta
     4921 atgaaaggta gcgcaactgc cccagcgatt gaaaaaacct tcgttgctta tactttttac
     4981 ctacaaactg ctgcatcaat ttattgattc gttgaccatc agcatccgcg ttcgagcccg
     5041 taaaaatcat agtcatatta ttaaacttct caagcgcgtg aaaaagttcg ttgcattctt
     5101 tttcgacgtt gccatttgca atcgttgtcg ggtgatatgt gatgatcaaa ctattaccaa
     5161 gtgtcggcaa attgagttcc cggcacaact ctttttcgct cttaagttta agtgagcgga
     5221 tactttctat gccgatcgcg ccgacatgaa aaacacgttc gggatgctca ccaagttgaa
     5281 tcacccgatc tctatatacc tcggttgaca caaaatgtaa ggaactcatc tttgttatag
     5341 catggcgaaa accgtcatca atcgaaccct ctgtcaattc gccaccgtgt aaatgaatga
     5401 tcggtattct caaaacatgc gccgtagcgg cagcagcaaa cgcttcaaat cgatccccta
     5461 aaataatcat cgcatccgga tttaaacgcg acaaggcgtc gcttagtcca atcatgccga
     5521 cacctattga tttagaaaca ccaatttgtg acatcgaatc gagcagcatc tcaacacgct
     5581 catgaatttc aaaaccatcg gattctattt cagactcagt cattccaaat ccttctgaca
     5641 aatgcatacc tgtgaccaat acgcataaat caaaattttt tgactttgaa agagcaaaga
     5701 tcagaggacg caaaagtcca tactccgatc tagcacctgt cacaacacac aacttgtgcc
     5761 ttttttgttt tttcatatac tgatcaaatc gtcaagatta aaatcttttt tggcctgacg
     5821 ccctatcacg cgattccatt gcatgggact aatacccgtt cctggtcgtt ttactgttat
     5881 gttcttttca gaaaaaaaat cacctttttt tatatcttgt gaagcaacaa tactttttct
     5941 cgcaatttta atattttttt tctcagatgc tgaaggtttc tttgtacttc tccctaacgc
     6001 tgtttcgata tgccgaatgg cgtcaaccat tcttttaaaa tcacgaggct ctatactggc
     6061 tttatgatca ggaccactca tgccacaatc caaagtaaaa tgcttttcta taaccgtagc
     6121 tcccaatgca acggctgcta tagcaacctc cgtacccatt gtatgatctg agtaacctac
     6181 gttaacgcca aactctgagc gcaatgtttg catcgcccta aggtgcacat ccctgtacgg
     6241 tgtggggtat tcggtattac aatgaagtag cgtaatgaac gaacgtcgta taccattttt
     6301 ttctaaaaga cgtattgctt tttcaacttc gtctaatgta cacatacctg ttgataaaat
     6361 aacaggacgt ccaaactgag cgattttttt gagatataaa taattagtga cttcaccaga
     6421 aggtatctta aaaaaaggca atttcatttt atgtagcgca tcaaggctct cgaggtcaaa
     6481 agccgttgag agaaatagaa ctcggtttct ttcgcactcc ttttgaagga tatctaattt
     6541 cgaatagggc agttccaatc gttttaacat ctgaaactgg ctttcatctg cattatctcg
     6601 tttcttctga tatttggctt taggcgcttt cgtcgttacc aaactttcag tacgaaatgt
     6661 ttgaaattta acaacgtcgg cacctgcttg tgcggcggct cttatcattt tttttgccag
     6721 acgtagctct ccgttatgat ttacacccgc ttctgcaatt attagtactt tattcatata
     6781 acctcctagc cggtactccg acataggttc ccgattctag tatatctgaa acaacaactg
     6841 agcctgctcc gataatcgta caatcgcaga ctgatacccc atgttttata actgcaccac
     6901 ttccaataaa cgtttcttta cctattttac agttaccatt aactattgcg cctgtcgaaa
     6961 catgacaaaa atcgcctaca ataacatcat gctcgataat agctccagta ttgataatac
     7021 aattaatacc tatatgtaca ttcgcattta ccacaactct gtgcattaca atagtaccct
     7081 gagatataaa tgaattatga gccacatgag caaggctaga aataatcgtc gcgaataatt
     7141 tgttgtgctg ttttagagtg tttgcgatag attttctttt ttcacaagac ttggtttgac
     7201 ccacagtaat cagaaaataa tccgggttta atcgatccaa attttgagtg gattcatcac
     7261 ctagtattgg atagtttcca attgatgacc ttgatggatc agtatctatt acacctaaaa
     7321 tatccacccc ttccagtgtc tctataacat caatacatga tctacaatgg ccgcctccgc
     7381 caacaagtac aagttttatt ttttcagaca ttttttactc ttggagaact aggtatatta
     7441 actacgcgat cttcaaacca ttccgcattt ggtaaaggag tcgtttgaca tttttgatac
     7501 attggcaact tattcattaa tgtccaaatc ggacgtgttg caatattttt cttattggta
     7561 aaactcaaaa actcatcacg ttcagcccga tctgataata tgatcgagtt tagccagtag
     7621 ttagatgttg taccctctct ctcttcaaaa aaacgcactt ttgtatcctt gaaaaaatcc
     7681 ctgtatgcat acgccagctc cctcttatcc cgtaataaat caggtaaacg ctccatttgt
     7741 gcacaaccaa gcgctgcatt tagattaggt aaacgaaaat tatatccgac ttcatcgtga
     7801 aaaaattccc attgatgcgg tcttttggct gttgtcgtga gatgcttggc tcgaagtgca
     7861 aactgtttat ttttagttat tatcattcct ccgccgccag ttgtaatagt tttatttcca
     7921 ttaaaactta aaactcctac atcccctatt agtcctgtat gggtatctcc gcaataactc
     7981 cccaaagatt cagcagcatc ttcaatcagc ggtatattaa atctatcaca aattactcgg
     8041 atattttcta tctccacaac atggccaaag gtatgcatgg gaatgcacgc cgccaaccgc
     8101 ctacctgttt ttctgttata tgattcccct tttatgttga tcgcgtttgt ttctaaaaat
     8161 tcatttaaac tgtctggact caaccccatc gttgatagac tacaatctac ataaatggga
     8221 atagcgccca catgagcaat tgcattgctt gtggccacaa aagttaaagg ttgcgtgatt
     8281 acctcttccc catgacgcac tccacttacc aataatgcta tttgaagagc aacggttccg
     8341 ttcgaggtcg ctacagcctc cgtagcccct gtataatctg ccaccatact ttcaaacttc
     8401 gtaacgtatt ctcctacaga ggaaacaaat gtagagtcaa tagtttccaa tacatacttt
     8461 ctctcgttac catgaaaaac aggtgcatgc agaggtattg gttgatcagt gtcataccaa
     8521 gatcttataa aatcaactac ctcatcaaag aatactttag acattatata tttcaggttt
     8581 gtagatattt aagtttttct ctataaacga gatagtctct tttaggccaa tttccaagtt
     8641 atacttaggg ctccaattcg tgttttttaa taatttagta ttatcacacc acagcctttc
     8701 tacctcgctg tgctcaggcc ttatccgtac agaatctgat tcaaattcaa attcaacctg
     8761 catcaatttg ccaatttttt ttgccaaatc accgatactg atttcaaacc ccatcccgat
     8821 attgaccgtt tctcccttta gcgtttgaga tcgaaaggct tcaacgaatc ccgcggccgt
     8881 atctttaacg aaagtaagat cgcgcgtcgg attaagactt ccgagtttga tactccggcg
     8941 cccagagaga agttgagata ctatcgtagg tatcactgca cgcgctgatt gacgaggacc
     9001 aaaaacattg aatggtctca ctatcgttac cggcaaatta aatgaacgat aataacttat
     9061 ggccaactga tccgctccaa ttttagtcgc tgaatatggt gattgaccta ccaaaggatg
     9121 agactcatta atcggaacat actgagccgt accataggtt tcgctagtag acgtaataat
     9181 tattttatct aaccctagac acttggaacc ttgtagaaca ttgtaagtcc caatgatatt
     9241 ggtgtcaata tatgcctttg gcgaaacgta tgaataagga atacctatta aagctgctaa
     9301 gtgaaaaaca ccaacgcagc cttcgagcgc tgaataaacc gaatcataat ctcgaatatc
     9361 acccgaaact atttccaaat ttgctttttc ttctaacgta tcaagccagc cccattcgtt
     9421 ttttgaatta taatgtacaa atgcttttac cgagaaaccg gaggcaacgc ataattcaac
     9481 taaatgagaa ccaataaaac cgcctgcacc tgtaatcaga accttatcca tgcaaaacac
     9541 ctaaagttat agaaatcttc aatgaataaa ttatttagta gagcccttca aatgaaacgc
     9601 tttaaaaatc gcagttactc gattttgttt cgacatatcg cttacgcgaa tgatgtcatt
     9661 cacaacatag aaaagcgatg taagaaccaa agataaaaat ccaactataa ttgtaattat
     9721 tttctttttt ggtttaaccg gatatgccgc cggcttacca tagtctaaca cctgtacggt
     9781 aggcgtgtcc tttgcttctt gaattttagt ttgttcaaat tgagggatga ggtattcgac
     9841 aattttctga ttaatctgta cctcacgaaa tagccgtaaa tactgcaatg cgatatcggc
     9901 tgtactatta aaaggaataa ggatactttg attaaaccca ccgacatcca ttttgttttg
     9961 aattcttcga agttcgtcca atcgtgattt ttgacgaacg gcatccggat gattatcgcc
    10021 aacgcttttt ctaattatat tgtattcagc ttcacccgtc accaattgag cctggatatc
    10081 tgctgccgtc tcaatggatg cttttgtttg ctcggttagc gctatgatcc cgtgccgttt
    10141 ttgaaaaaaa ttgaggctat cctctgcttt ttttaattct atccgagaat cgtaatacct
    10201 tttttcaata aattggcgcg agaatcgcgc ttggtccgtt gataattcac gattgattct
    10261 atctaattca gaaacagcaa aattagttat atctgctgtc atctcaggtg atccttgaaa
    10321 tctcacttgg atagaaatag ttgaattttt tacattactt tcaaaaaaaa tagattcctc
    10381 catcaactca agtaaagcag atgtgtcttt ccttgttggc tccatttcaa aaaaatcaat
    10441 cagcttaaat cgagctataa ttgcttcttt taatcgctga ctacgcaata ttgccaaata
    10501 tcgttgcgga cctgaaattc cgctacctga aagcaatgtc tgacctattc cccctaacgc
    10561 tccacctaat gactcaatcg cagaggatga tttatcgggt aataaaatga cagcctgaga
    10621 ttcataccac ggtttggtta aaaacaaaaa cgctactgtt gctaccgtaa taaaaaaaga
    10681 tgttactatt attttttttc gatacggata cagtttttcc atccaatcac caagtgattt
    10741 agggaaagca gcttgcccct caatgccaga gtcatttgtg ttattggaat tgttcatgat
    10801 atttctgacc ataagaataa aacatgcata gcgatgagcg agttattaac ggccttataa
    10861 ttgtaacaac agtgtcaatc gatcaaattc attattgcga atacagcttt ccgtttcgta
    10921 ctgtcgttca cgctttcgcg ggaacgacag tacggcggca cgctgtctca cccgcgtaag
    10981 cagatgtcta tgcaattatg agtccgttaa taactgacaa aaacatcaaa tgatgtacgc
    11041 tcataccaac taccaactta atttaagacc taattcaaaa aaatatccat ttaacgtatt
    11101 ttacgacttt ttttcttcaa aaaagagaat gcttatattt tattgaatga ttaatttttc
    11161 atatgcgata ctaaatttag cattaaccaa ttagagtaat tatttaataa aaaagcctaa
    11221 caaatacaac cttttacatc gaatccgatc tcacccacct tccaaacacg atcccccgcc
    11281 aatatcgccc atcgtaagct tttttaccgg caatcatcgc atcaaaatca tcccgtagcg
    11341 aagatcgtat tacccccccc gagcgctcaa tcgaagaact cagttgcttg cgaaatacat
    11401 ccggatgtgt acgaacccaa tgttcttccg gcgttacaaa tcccatcttg tcacgacgct
    11461 gaataatact ttccggcaca taacctttca tgctgtcacg taatacacgt ttggtcgttg
    11521 cattcgaaat tttatgatca gacggtaacg ataagataaa ctccaccagc cgataatcca
    11581 aaaagggaac acgcgcttcg atagaatgcg ccatcgaatt acgatcctcc caatgcaata
    11641 acatcggtaa attggtacgc gtcaattgaa cgcgaagcgc tgtatttaca tccggtttaa
    11701 attctccata ttgttgccgc caatgtattg aacgcgctcc gagtttttcc atgtcaaacc
    11761 aatcattgga cgattggcta tccgacggca taaacggcat ccatgcgtta ggtataagcg
    11821 ctcctatcaa tcgccgtaag acagcgccgc ggttgacatg gccttcgcct atagtttttt
    11881 gaatttcgct aattaagcga atccaacgcc cgcgcaccat gagttccgct aaacgcgtcc
    11941 cataaaacgg gtagtaaccg gccaactgct catccgaacc ctgaccgtcc agcatcaccg
    12001 ttacaccgcc ggatttggcc aatcgaaaaa catgccattg tgcgtaaata ctcgctgaac
    12061 caaacggttc gtcctgatgc cacagcaacg tatccaacgt atcaaacagc gtttcaaaat
    12121 ccggataaac atgatgcgct tcgacacgaa tggcacgaat cacttcttcc gcataacggc
    12181 tttcatcgta tgcctgctcc cgtgcatagg ctgaaaaagt tttttgcaat gccgtcgtat
    12241 tctgcgtgga cagcatatca tgcaacaaac aaacaattga agacgaatcc aatccgccgg
    12301 agagacacga acctaccggc acatcggagc gcaaccgcaa ccgtaccgca tccgtaaaaa
    12361 catttaaaaa ctgtttcgtc gcatcctcat acgtaccgtc aaactgtgta tccggaaaag
    12421 aataccaacg gcgtttttca aatcgtacgg tttgagcctc gtacgtgatc tccgcgcact
    12481 ctcctccacg aagctgcaaa accctatcgt acatggtttc atccgtatga tcggaaatcg
    12541 cataacccaa aaaatcggct acgcgttgtc tattcatcga atatcgccat cccggtaaaa
    12601 cggagaactg cttgatttcg ctggcgaatg cgtaccctcc atccgttgtt ttataataat
    12661 acaggggctt aacaccaaaa cgatcccgcg ctacaaaagt acgtttggtc agcgtatcat
    12721 aaagaacaaa cgcccacata ccattgaaac gatgaaggca ttccgttccc caatagtcgt
    12781 acgccgcaag gaccacttcg gtatccgttt tggatgcaaa cacatatcct accgcctcca
    12841 gctcacgacg caattcgatg taattatata cttccccgtt gtacgtgatc caataacgtt
    12901 catttttata actcatcggt tcacggcctg cgttgctgag atctatgatg gcaagacgac
    12961 gttggccgag ccacacacga ttcccatcaa aaccattatg cccgtcaccg tccggtccgc
    13021 gatatgaaac gatatcggtc atacgctgaa tcagcgcacc gtccgtcggc ttcgtattga
    13081 taagcgccgc tattccgcac atcgctgaaa atactccgtt ttgtaattaa cttgcttccg
    13141 gctggacgtc acgatcataa cttcgcggca ctcctttttc gttttccgcg gcgtgtgcgc
    13201 gttcgtgaat tgcttttttc accaagatat cacgtttagc gtgcggccac tgccggggct
    13261 tgagcatcat aaatataaaa ctgtacaata ctcgtttaac cgaaggcggc aactcgatca
    13321 agacaaaaaa catcactaat gccaacccgt tacacataga gattgttcgc atatgacgcc
    13381 gcacattttc acgcaaaaga cgaatctggc gaggactgtc aaaaaacgca gtatatagca
    13441 tgacgttttt taaataaaaa aaaaggcgcc gggtccaaaa taaatgtcgg caaacagcac
    13501 gaaaaaatga cgtccctaat tcaggcttta aaatcaatgc gacgtgctgc aatgaacgaa
    13561 tcccagtttc ccacagatac cacccggaat ataacgtatt ctcagtatgg ttgggcgaat
    13621 acgaactgat gagcggcaac gaaagaaacg gtttggataa attaccactt ttcatggcta
    13681 aaaaagcaat atgcgtttgc ggccaatacg actgtgcgat caatgcatca tcacccggtt
    13741 ttaacccctg ccgggcatgc tcggtgcgaa tcgcaaaaac accggcatta cccatataat
    13801 aaaaatagcg ctcggcggcc gattccaacg tcataaggct ttcgtggcct atgagtttcc
    13861 attgatcaaa catcactaca gaaaaatcat cacgcgttgt cagatgatgc atgatttcag
    13921 gaagtatgcc ttcagccaga atatcgtcat caccaaatgc catccagaac cttccgcggc
    13981 tttgatgaaa cgtttgtatc atatttcgga tggcaccgat attttccgaa ttgcgtacat
    14041 aacgaatcgc tacatttgtt tgtgcgctca tcgcctttac gacaacgccg gtatcatccg
    14101 tagaacaatt atccgaaacg ataatttcga cggaccgggt aaaatcaaat ttttcggctt
    14161 gatgtataat actctcaagc agcgctttca gaaatccgga gcgattccat gtcggtacgc
    14221 cgatagagag aattatatta tttttcatta tgcacgcatc cttaccgaac gaacaaccca
    14281 aatcaaaaaa acaatcaaca tcatcgcttc accgatgctg tataagatta cggtggtata
    14341 agccgagccg gaggtcacac cgataccgat catggccgca cgtatcgcaa aacttagaag
    14401 tgaaataaca aaatgcatac gctgcttgcg aatcaccgac gaaatcatgc ttacgggtga
    14461 cgcgacaagg tttactgcaa tcgctatcac aagggaccgg gcatattcgc cggcatccct
    14521 ccacgcagca ccaaaaaccc attcgaataa ccaaggaccg gcggccacaa tgataatcaa
    14581 aaacgggaac gaaaaaaagg acagaaactt aatgttattg atcacaaacg aacgcaaaag
    14641 tgtcgcatct tcacttagtt tggctgacat ttcataaaaa acttgcgcca caccttgtcc
    14701 gatcaccgta cttggcaaac gaagcacatt ggttgccaac gcaaaacagg ccgcatcgtg
    14761 aggactgaac gatgccgtga atattaacaa cggcaatgac gccgtcagtt gatcaagaaa
    14821 cgtcatcagc aaaccatacg tcggaaactg tttaaagcgt tttagcaaat ttcggatatc
    14881 cgcccaatca acagcaagat ggcgtattgg cttcccttcg tgccgcaatc caaaaaaaat
    14941 cgtgatgtat aatgtgatgg ccgccgatat atgacccaat accaagcccg tgatcgtcga
    15001 accttgccac gaatacattg acagcatgac aataaacgcc aacagcggcg gcaaagcacg
    15061 cgagaagctc aataccctat acgccgcata acgattggcc cagctcgcaa gcaggatatt
    15121 acctgcgcct aaaataaccc acaccggcaa ccaaaaatat agcggcccgc ctttggggaa
    15181 aaatcccaaa tccaacgcca agccataaaa caccactgca ctgatagcga ccactatcgc
    15241 gaaacccaaa atcagttgcc cgcaaaatcg aatgatcttt gcagcctgat catcggagtc
    15301 cgccaagacg atcgccgaat ccatgcgaag catgattgtg gaagacgcaa tcatcaccat
    15361 agcattgagt gttgcggcga tcccgtaatc cgctgaatca taaagccgtg ttacaatcgg
    15421 caataaaaga acagcgatcg cctggcctat gatcgttcca atggacaatc gggacacatt
    15481 tttgaggtaa ccgctttgtt tgatgcgctc taccaattgg cgtagcgtca taggaatgga
    15541 tgatggagga gcggacatga taattgcgct atatgtattc cgacaatacg agtccgcaag
    15601 attgccgcag atagtcttgc gatccggcga tttgtataaa ctgatcggga attgcaatgc
    15661 gatgtacgag cggtatcggc atggactttt tcttttcacg gcaatcatgc aaagcttcga
    15721 ggacggccga acctaaaccg gcactacgct gatgttcttc gagcgtgatg attcgttcgt
    15781 acgaatgtgc caatagctct atagtcgcgc tgtcaatggg tttgatatac ggcgtactat
    15841 acaaacccca aagcaaatta ttatgacgaa taaaatccga cgcgtaacga agcatggcgc
    15901 ctgttgttaa cacgactgta ttttttccgt gacaaacagg aatcatttta ccggctgcga
    15961 atgcgatatt tccgggatgt acagccggtt cgccggcttt tccaagtcgg atatacatcg
    16021 gtgcatcggt ttgaacggca tgggtcgtga tgaggcgggc ttcctgagga tcacccggcg
    16081 ccgtaacaat catactcgga agcgtacgta aaattcctaa atcttccgtt ccatgatgtg
    16141 aagccccaag cgcaccgtat gcaaatcctc cgccgaccga tacgattttt acaccaagcg
    16201 catggtaaca aatatcgtag cgaatctgtt ccaagcatcg caacgtggga aaattaccaa
    16261 tcgaataaat gaacggaata tatccttctt tggccaaacc tgcggcaata ccggccatat
    16321 tttgttcggc cacaccgata ttgagaaacc gatcaggaaa cgactcggca aacggctcaa
    16381 ccaccgaata gccgagatca cctaccaaca aaaatacgcg ttcatcacgt tttgcttcgc
    16441 tcatcagttg ctgaataaag gccgtacgca ttacgaatgc tccagttgtt ccagcgcatt
    16501 acggaattgt tcagcgtcag gtgtacgata gtgccaaagc aatttttctt ccataaaatc
    16561 aacacccttg cctttgactg tctcagcaat aatgatatgc ggtttatcat ctgttttttt
    16621 cgttatcgcc gccgataacg cggcatgatc atgaccatcc gttacggtca cataccatcc
    16681 aaaggatttg aacttttcag ataaaggccc caagtcaagc acttcattca catgaccaag
    16741 gctttgtatt ttattatgat ctacgatcac caccatgttg ttcagatgat ggtgcggtgc
    16801 aaacagcaat gcttcccaat tggatccttc atccaactct ccgtcgctaa ggagcgtaaa
    16861 aacacgccac gcttgcccgc gtcgtttggc ggccaacgcc agcccgcaac caaccgataa
    16921 cgcgtgcccc aaacttcccg tggacaattc tacgcccggg atgtgatgat tggcatgcga
    16981 agtaaaaaaa cttccatttt gcgcaaagga agtaagttgt gacatgtcaa aaaaaccgcg
    17041 caacgcaagc gctgcatata aagcagagca cgcgtgccct ttactgagaa gaaaacgatc
    17101 ccgctcaggc caccgtgggt ttttgggatt gacattcatc acaccgccgt ataaaacagc
    17161 taaaatatcc gccatggaaa aagcgccgcc aaggtgcgac gaattcgaag catgaaccat
    17221 ttttaacgct tcgatccgaa tcgctttgct gaatgctttt acttcatccg tggtcacgat
    17281 ttcacccaag tcttgatttt atcggcgtgc cgttcacgat gcaaaaaaat aaaattggtt
    17341 tcgacggtcg tttcgtccac aaaggttatc ggtgcgagat aatcattttg aataaaaaaa
    17401 cattggtatc ccatttcctt aaaaaaagta atgatgtcat taggatgata attgtacttt
    17461 gccgcccact tacgcaacat ttccgtacat acgatcggct tatgggtttg cagcgtgtat
    17521 ttccctccac gatagaccat caattccgcg ccttccacat cgcatttgat aaagtccggc
    17581 ttcgcaccat actgtttaca aaaattatcc atcgtttcca gcgcacattc tgtttcgtgt
    17641 gcattagcgg cttcggttat attgacagcc gatgcactgg tcgaaagttc cggatcgaca
    17701 aaaaacgtaa gttttcctgc gcgatcggat aagccgagat tatacgactt aacacgcatg
    17761 cctgtattca gctgaatatt attttccagc tccagatacg ttccgggtac cggctcaaac
    17821 gcacaaacat gcaaatcgga aacggcctta gaaaaattta gtgaatacca gccgatatta
    17881 gcgccaatgt caaaaaccga catacccggt tccaacaaat ggcacatcat ggaaaaatac
    17941 tctggttcgt actgcgcaaa attaagaatt tcaaacggcg cactgcgttt gtcggctttt
    18001 ggggttaacc aacgaagccg gtgcggacgc gtagtccata ctataccatg cgacgtgatc
    18061 gtgatatttt cgatatccgt gtgccggagt atatccgtgt attcgaaaag ctgattatgt
    18121 ttctcataca tcgcttgtat gtattccgat tttttaatcg caccggaacg gtattgattt
    18181 tgaatgtctg taattttcat gtataggact taatcgttcg tgtaaaaccc tcttcgacag
    18241 ttgtcgtcgg ctgccaacct aagctcttta ttttggtaat atccggaaca ctgcgttgta
    18301 cggcgctggg aatatagcca ttttgcggcg ggatatccaa aaccactgat aatttttttt
    18361 ccggaaacag tgttactaat ttttccgcca atcctcgaat gctgatttca cattcagggt
    18421 tacctacatt atatgcttca gttgtttttc cgtggagcat aaccgtaaaa aaaccgataa
    18481 tcgcatcgcg aagataacaa aaggagcgcg ttgcagcacc gctactacga agtacaatat
    18541 tttttccatt aacaatatct gaaaccaaat ccgcaaaaat acgtccgtca tccagcgcca
    18601 tgccgggccc ataggtgtga aacggtcgaa cgatcaccgt ggaaagacca tactggtgag
    18661 accatgcaac gcacattgtt tcaccaagcc ttttgccttc cgcataacat gaacgcagcg
    18721 ccatactgtc caagcaacca agctgcatct cattggtcgg tatttggcgc gcttccagtt
    18781 gaccatatac ttcgctgctg ctaaagtaca aaaatcgttc gctttgggat tgacgcgcca
    18841 cctcaagcat gttttgcgtg ccggtcgtat tggccgtcaa agtacccacc ggatctatac
    18901 cataatattt tggcgaggct tggcttgcgg catgtactac gaaatgaacc ggcgtatcag
    18961 agcgaaatac caaccgtgtc acatcggcta cgtgtaactt aaaatcgtta cgtccggcat
    19021 gatgtgcaaa cctctgacgt gcacgttcct cggatcgtac aatcccgatt acatgaatgt
    19081 tcatatccaa acgatcattg agtcctaata acgtttctac catatacgcc ggtagaaaac
    19141 cattggcccc actgataaga acagtttttc cacggagtat gtcccaagga agcggcgcag
    19201 aaataatatg cgcgatgtct tcgcgtatta ttgaatgcat cacgaaaccg tatctcgtcg
    19261 gagaaatgaa cggatagcgt cgatcatata atctaacatc ggggtcgtta aaccaggata
    19321 cacccccaac caaaacccgt tattcatgat aagatccgta ttggtcaagt cacctacaac
    19381 gcggtaagaa cccggctgtt ctcggaaagc atcaaaaaaa ggttggcgta tgagattgcc
    19441 gccaaacaac atgcgtgttt gaattttttt gttttctaga tagtggatca actcgggacg
    19501 agaaataccg ctttcgctgc gaagactgat gagaaaacca aaccaactcg gattggtatt
    19561 gggtgtggct tcaggtaaaa taaaaatatc cgagaattcc gctaaaccgt ttttcagata
    19621 attccaattt gcacgtcgtc tatcggcaaa atcctcaagt ctgtctaatt gcacgcaacc
    19681 tattgcggct tgaatatccg tagctttaag attgtaacct atatggcggt acgtgtattt
    19741 atgatcataa ccgcacggca acgttccgag ttcccaatca aaccgtttac cacacgtatt
    19801 atcttttccg gaatcgcacc agcagtcacg accccaatca cgaaatgatt caatactgcg
    19861 tttcagaata cttttattag tatacacggc gccgccttca cccatcgtaa tatggtgcgg
    19921 cggataaaaa ctacttgtcg cgacatcacc gaatgttcct gtccaccggc cacgatattc
    19981 cgaacccagc gcatcgcaat tatcttctac gagccataga ttatattttt tacaaaacgc
    20041 cgtcacggca tccagatcaa aagggttgcc tagcgtatga gcgatcatga ccgcttttgt
    20101 ttttgggctg tacgccgctt ccaatgctcg cgtatcaata ttcgctgttt cggtatgcac
    20161 atcaagaaaa acaggtacgg cgccaaattg caacatcgga gcaatcgtcg tagggaaacc
    20221 ggccgctacg gttataactt cgtcgccttt acgaatccga cgatcttcca gtaacggtga
    20281 tgtcaacccc ataaacgcca aaaggttggc cgacgaaccg gagttgcaca acatagcatg
    20341 cttcacgccg agttttttgg ctagcttgct ttcaaactcc tttgaaaaat gcccggatgt
    20401 aagccaaaac tcaagacttg catctacggc gttttgcatt tctttttcat caaacacgcg
    20461 tcccgcatac gatatagcgg actgaccggg cacaaattcc tttttttgac cgtgcgtcaa
    20521 acggtaatat tcggccgttt tctctaaaat ttcgagccga agcttttccg cagcggcttt
    20581 tacgttttga tccatgtcgt ttattttgtt ctcgttttcg gagtcgcgct caaagttaaa
    20641 aaatcaaatc ttcagatcgt gtgggatgac aagcttagat attcatcgag ttgtttttta
    20701 cgtgcatccc aaagcgaacc ggcgaatgca tcgtcgtgat agccttgggc tgtcaatgca
    20761 atggtctgtt ccagtgataa agccggcttc cacgacaatc gtgaaacagc cttggatata
    20821 tctaaacgca acatgtaggc ttcgtgttca ttttgtttta ccgccgcggt ttcaaatcgt
    20881 ccgaaacctg ttttggaaat cagcgcgagg ataagttcct gtaccgagca cgcatgatgc
    20941 attgccggtc caaaattcca tccgccggag tatgttttac cgtggaggaa caatttctcc
    21001 cccaacgtaa gatagccgcg caacggctca agaacgaatt gccacggacg aactgcattg
    21061 gggtttcgca gtatcagtgt ttgatttttt tgtaccgctc tgaaaaaatc aggaatgatg
    21121 cgattctcgg cccaatcccc tccgccgatt acattaccgg cacgcgcact agcgatggag
    21181 cacgtccctt cgctcgaaaa aaaagattgc gcatatgaag acgtgatcag ttccgatgcg
    21241 cctttggaag cgctataggg gtcggcgcca cccataggat cgttttcacg ataaccccac
    21301 acccactcgt tattttgata acacttatcg ctagttacat tgattgctgc acgaacggat
    21361 gatattgcgc gcaccgcttc aaaaaaacga accgtgccca caacgttggt ttcaaatgtc
    21421 tctgcaggat cgcgataact gtccagtacc aacgcttggg cggcaagatg aaatgcgata
    21481 tcgggtcgaa cttctttaac ataatctgcc aatgcgtgcg gatcgcgaat atcaccatcg
    21541 cggtgatgta ttttattttg caaatcacat cgtacgaaat tatcgttatt ggatttcgga
    21601 gggagagcaa acccgtaaac ctcggcgcca agggtatgca accaaacact gagccaagat
    21661 cctttgaaac cggtatcgcc tgtaaccaaa acgcgtttat cgcgatagat gtcggaaaaa
    21721 ttgtttttca ccagatcacc catggtgcgc gcttttcacg tataagttcg tcgagcatat
    21781 tcttatcacg caatgtatcc atcggctgcc aaaatctgtc atgtttgaat gcaacaagcg
    21841 attgtgaacg agccaatgtt tctaacggtt cacgttccaa tacggtttga tcgtctttga
    21901 gtaattgtaa aacactgttt tcaaaaataa aaaaaccggc attcacccaa gccccgtcgc
    21961 ctttgggttt ttcctgaaaa ctctgaacca ttccggacga atccatagcc atggcaccga
    22021 atctcccgct cggctgtatc gccgtcactg tcgccgtttt gccgtgcgtc atatggaatg
    22081 acaagagttt acggatatcc acatcggcca ctccgtcgcc gtaagtcaga aaaaaacgtt
    22141 cgccatcaag ataggaagca acgcgtctga tacgtccgcc ggtcatggta tccagtccgg
    22201 tatccaccaa cgtcacacgc cacggttctg ccgtatgcgt gtgaatgacg ggcgcattgt
    22261 gcgcacgaaa atcaaacgtg acatccgatt cgtgtaaaaa ataattggca aaatattctt
    22321 tgatcacata gcccttgtag ccgaggcata tcacaaaatc attgtacccg tagccggagt
    22381 agattttcat aatgtgccat aaaatcggtt taccaccgat ttcgatcatc ggtttcggtt
    22441 ttaagtgcga ctcttcactg atgcgcgtac cgtaaccgcc ggctaaaatg acaactttca
    22501 tatcaagctt tcacgaattc agtgatggta ttgactactt ccgaaatctg ctctttcgtg
    22561 agctccggat aaatcggcac ggcaagcgat tgttccgctt gggcattggc attgggaaaa
    22621 tcggtatctt tttcaccgag ataaccaaaa cattcctgac gatgaaacgg caccgggtaa
    22681 tacacttcgt tgccgatttg tttatcggtt agatatttac gcaatgcatc acgtttttct
    22741 actcgtatta catattgatt atagatatgg tggtttttcg ctccggacgt gcggtatgcg
    22801 gctttgggaa gcagtacttt atttttgctg tcgtaagtta tcttaccttc cgcttcagcc
    22861 actccggctg caataaaaag ttgggtatac agatcggcat tttcacgacg ccgttggctc
    22921 cagttatcaa gatgcggaag ttttacatta aggacagcgg cctgaatcgc atcaaggcga
    22981 aaattaccgc cgatcatttt gtgataatat tttggtttac cgccatgcac gcgcaggacg
    23041 cgcaactttt caccgagagc atcgtcattg gtcgtcacaa ggcctccgtc gccaaagcaa
    23101 ccgaggttct tactagggaa aaaagaaaag caaccaatgt cgccaaaatt tccgactttt
    23161 tttccatcgc tgtattgtac gccgatcgcc tgcgcaccgt cttcgatgac tttgagatta
    23221 tgtttttttg
//
LOCUS       DAOQGF010000044        23168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1017_length_23168_cov_7.711937, whole genome shotgun sequence.
ACCESSION   DAOQGF010000044 DAOQGF010000000
VERSION     DAOQGF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1017_length_23168_cov_7.711937"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /gene="katG"
                     /locus_tag="PK937_07850"
     CDS             complement(<1..1806)
                     /gene="katG"
                     /locus_tag="PK937_07850"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNH32521.1"
                     /translation="MSEISKCPFHAAGRGTTNQDWWPNQLNIKILHQNSSLSNPMGKK
                     FDYAKEFKKLNLKAIKKDLYALMTDSQEWWPADYGHYGGLFIRMAWHSAGTYRIADGR
                     GGAGNGTQRFAPLNSWPDNANLDKARRLLWPIKQKYGNKISWADLMILAGNCALESMG
                     FKTFGFAGGREDIWEPEEDIYWGSETEWLGDKRYSGERDLENPLGAVQMGLIYVNPQG
                     PNGKPDPVASGRDIRETFARMAMNDEETVALVAGGHTFGKAHGAAPDSHVGREPEAAS
                     IEEQGLGWTSKYGSGKGDDTITSGIEGAWKPNPTQWDNGYFDMLFGYDWELVKSPAGA
                     WQWTPKNVADKDLAPAAHDATKRVPTMMTTADMALRMDPIYEPISRRFHKDPQAFADA
                     FARAWFKLTHRDMGPRSRYLGEEVPKEVLIWQDPVPAADHKLVNEKDIAALKKKISES
                     KLTVSQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAINQPEQLSAVLNKLEKI
                     QKEFNRAQPGGKKKISLADMIVLGGCIGIELAAKQAEQNITVPFSPGRTDATQAQTDA
                     ASFAVLEPVADGFRNYLKADYDVPAEELLIDKAQLL"
     gene            1980..2933
                     /locus_tag="PK937_07855"
     CDS             1980..2933
                     /locus_tag="PK937_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNH32522.1"
                     /translation="MTLLQLTYIVAVEKYRSFSKAAKDRFVTQPTLSMQIQKLEEELG
                     VTIFDRGKKPVAPTDIGRTIIEQAQAILRESDKIIQSVESETTEVAGIFRIGIIPTLA
                     PYLLNLFLQDFITKHPRVELVIEELQTREITERLNRSDLDAGLLATPLHHKNLAEKPL
                     FYEPFVAYVSEEHRLSAKKKIHIKDLQNGDLWLLTEGHCFRSQALALCKQKKNITPKH
                     KGALRFESGNLDTLRKLVEQNYGMTLLPYLAVSELSDEKKKLYMRPFAPPAPKREISL
                     VYNRSYPKKKILEVLEHAIRFSVPEEILEKDRSLIVHHGAV"
     gene            complement(2942..3658)
                     /locus_tag="PK937_07860"
     CDS             complement(2942..3658)
                     /locus_tag="PK937_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNH32523.1"
                     /translation="MIYRITKDLFQMFMILSILTSCEEKKEFQNAAYLSISAFADSIK
                     KNPDALLVDVRTYKEYMSGHIPGAQNVNWANVGTFDYHAAQWDIGKPIFLYCSVGGRS
                     TGARRKLRNMGFRRVYELDGGFVNWRAKGFSETTLNERGGMSRSYFDSMITSDRPVLI
                     DFYTTHCSPCRLMEPIVNEVAEKSDIIVVSINIDDHTLIANDLKIHTVPTFHIYKNRR
                     LTWSHIGTVNKEKLLEELLR"
     assembly_gap    3937..4036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4037..4465
                     /locus_tag="PK937_07865"
     CDS             <4037..4465
                     /locus_tag="PK937_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH32524.1"
                     /translation="ECSRRMFSSNVLVECSRRMFSSKQRRKGLEKEGTRGVAELAESA
                     CMCERHLRRKTKEKFGLPPHRLLENIRLAKAIEAIHEQPEVALFQISQIAGYASYKSF
                     YQAFTRRLKVTPSEAKQHLKQNPSVTMYALREKIEKKLTI"
     gene            4644..5963
                     /locus_tag="PK937_07870"
     CDS             4644..5963
                     /locus_tag="PK937_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32525.1"
                     /translation="MRIFSLLITLVFLLMNCKVIAQNQTLEQSVMVDMINPRSLDHPI
                     YLSFKDGDYLTSIIECDKWDSIYRGTNSEDAFMEQEAYYNSFVGNYQKALENFDKINA
                     KWINQSDTTNLLELSFLSAKKVDAVNEILREAVNHQITIVNEAHHMPQHRAFISSLLK
                     PLYESGYRYFATEMLTDNVDAVKKDGYVSFQQNGFYPNEPVYANLIRKALEIGYTLVS
                     YEDTVNCDFDPTNPLACFNQREQNQAKNISNLILKNYSNAKILIHCGYQHVDEIEHQS
                     PKVKTMAAHLKELSDSDPLTIDQTVMSEHGSVNFENFYYRYAQDSLKLKSPTVFKSNS
                     GYWVDDTKRGFVDMQVFHPRTNYINGRPNWLIQENHILTLPDFDISSFPFLVQAFKKS
                     DKTHSIPMDQFLVNSRKKIAVAVPKGYYRIRIINNKGTIHELEVKNP"
     gene            6024..6293
                     /locus_tag="PK937_07875"
     CDS             6024..6293
                     /locus_tag="PK937_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32526.1"
                     /translation="MALICYSDSNFTGAANFFIKATKIDTTDGGLYYNVACCFSRIGK
                     IDKSFEWLNVAIQKGYNDYQWINNDPDLFNVRQDRRWEEFIKIKK"
     gene            6305..7117
                     /locus_tag="PK937_07880"
     CDS             6305..7117
                     /locus_tag="PK937_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32527.1"
                     /translation="MNINIALIGMIVLILLETGCSQTHSFMSEKIEACGLSDSLMIDE
                     KQLFKKIDSLIEFSRCDTSVIFGMNLFKKNEKKSDWIAIKLYARRPKQRVLDRLEMLK
                     KSDQLYRKKIIDSIGPSKGWASEEVRQLWIKQAEIDSLNWLTFNSKIIDHYGWPKISE
                     YGYEASATAFLIVQHTNAKNQEQKLPLLMKLADEGEFSMSEIALLIDRIKVSKGEFQV
                     YGSQVEFDAISKKFRFLPIEDEKNVDVRRTKVGLPPIQEYAKRWNIDYSYIK"
     gene            7470..9056
                     /locus_tag="PK937_07885"
     CDS             7470..9056
                     /locus_tag="PK937_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32528.1"
                     /translation="MKISIRFFGDYTFNTPSDWDSVKTTKLALQRINKINDFSLSESD
                     ILVSTRTVIEPYFSSIISLIPSSKIRFDHLKSFDSLGTDKQGHALYQNISPQDSFCLF
                     QKLIKLNNYWLHVTGQTINSTDGSCYSSSLVDEYNKISNSIEYIKRSERAALKNPYLI
                     ADSLFNSDSNGNYIKPIDYLKSEKNVYENSSLRNSYVQCLETYKSFVGMTEPSLVPIN
                     VYFPNPTNSFEPFSSEFGGIVDTIISLTSNNKIIIFNESHIYPSQRLLLSGLLNKLYE
                     AGFRKIALEAVWNDKEINAIGFPTLKSGFYTREPQMANLIRKAKEIGYQVISYEDPTL
                     GNREYNQAVTLSREFRKDSTAKMIVLCGSYHILEKRSDSSRTWMAEHLKEISKIDPLT
                     FNTVFFENVYFDNPRLLSLISGSKLNEIAPVGFNFVNDYYLFNVTSLKPFTCDNEKGN
                     FAPYKPIQIVVPQLIKKVLLVQIFSSKEYGIRGRVPVASHYVDSENCQMTYWLPPDEY
                     VVVYYGASGEIILVNKIILS"
     gene            9567..9737
                     /locus_tag="PK937_07890"
     CDS             9567..9737
                     /locus_tag="PK937_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32529.1"
                     /translation="MKTKEKMEVIDSPLFQTIEREQMKSVQGGKPPGWTMAEQTVYSN
                     GTSSEDSAAGND"
     gene            9822..10778
                     /gene="gwsG"
                     /locus_tag="PK937_07895"
     CDS             9822..10778
                     /gene="gwsG"
                     /locus_tag="PK937_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system ATP-grasp peptide
                     maturase"
                     /protein_id="HNH32530.1"
                     /translation="MVLILTYDGDTTSDYIIEVLKNKKHPYLRLNSSLFFKEKITIAP
                     LHKFIEINGFKYTSDLLRVIWFRRFYSYRNLKFIYPKRIVNKSDFPNLTFEFNKIIRT
                     LYATLNSSHWLLDPRHAFVNKIEVLQTAKEIGLLVPNTTIINNKAFLSKKENSNLVSK
                     SIAEGVGVKAKRYNFSMYTKTISPDFISKLPSLYVPSLVQERIKKRYDIRIFYLNGRI
                     FASAIFSQNNERTIDDFRIYDDNRPNRIVPYKLPNAIKRGIRRLMKKLSLNIGAIDMV
                     RSQTGEYYFLEVNPNGQFGMISHPCNYNLFEVVADYLIRNDK"
     gene            10810..12519
                     /locus_tag="PK937_07900"
     CDS             10810..12519
                     /locus_tag="PK937_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32531.1"
                     /translation="MLLVGCSIPTNQFISHNRFQKTMGRVVYHNDSLRLSAMFYGDYV
                     FHSGNKQREISRKSIEIIKSGYKEGLYLTEKDLIFTAETIVEPKFYSALFSLHNLSKR
                     RLNEISKIAPDFFWSDSVRNVAGIGQIFTDATSAVFLIGFTYDHLTVRNKNSLQQQFF
                     FNQTLNALSREFRDADESIFKSVLSGSNYKLFEAEDPFILANKYYQTEYSGNYLLPIN
                     ELLESEPNYVSTQDRYKYLQALLTYMSFIDHPQYDYYNKKRKQIQSRSIINYSKRFNL
                     NIDEIQKTILDSSSHQRVVMFNESHYVPSIRSFIMTLLDGLYQQGFRYLALEALEEDR
                     LVNEKKFPVISSGFYFRDFRMANIARMAKKLGYHLIKYDSRETNREEKQAQRLAQLIA
                     NSDTNKIVVLSGFSHIDENPDKKRMASILKKISGINPFTINMTDISETRIELNSNICF
                     TNNSTDRIQLPSYFMNNDLYVSICNDDFINDAFTFHKHETLNIHLDFSGIAKRGEYVL
                     LVFDISESPNEGQLPVFAKLVLPKMHEQIDIKLLPGRYNVLLKSEDGLICHDTTLYVE
                     VNK"
     gene            13519..13884
                     /locus_tag="PK937_07905"
     CDS             13519..13884
                     /locus_tag="PK937_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32532.1"
                     /translation="MSKKFSLALVFYVCFATVKSQDIKNIDEQATALYLKKEYLKCAE
                     TFQKAIAADSNYFSAYYNTACCYTLAGKFELSIKFLKQAIDRGFSNERMLKTDPDLNQ
                     LRSRDEWKEILEYFHKVRY"
     gene            14183..15409
                     /locus_tag="PK937_07910"
     CDS             14183..15409
                     /locus_tag="PK937_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32533.1"
                     /translation="MDSIRDIDRSLSSDRNYIAALTRLNRIHAMPQYAGQPILFNHLG
                     LYNSLVSDYSTAVAFFDSIELLRQSQRHTTSLPPTGLTFDTSQIRPFPAMETILRLAK
                     NNSVVMINERHHMPMHRAFILELLIRLKALGYTHYAAEGLQNHDTITSPATYPAIGKT
                     GYYVNESVFGELIRTAILLGYKIVAYETTSSASPESDPIKNQNKREKEQAENLYNKTL
                     RQDPHCKVLVHVGYGHNDKMGVGGWIPMASYFKTISGVEPLSIDQIEMFEHRVPYFEN
                     PYYRFISGMISQPSVFIENSSNTFWSYLQRGSYDLEICHPKETYVHGRPTWRNIANRY
                     RMYDLPISKKTIAKGLLQAFYHDEPSDAVPADQIVFNTGVQQMIPCLFLQPGKYRFKL
                     VSEDSTISTEWVDIIK"
     gene            15412..17613
                     /locus_tag="PK937_07915"
     CDS             15412..17613
                     /locus_tag="PK937_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNH32534.1"
                     /translation="MNSFPIYLQHDKMDCGPACIRMITKFFGRTVSPEKLRRITYASK
                     GGTSLLNLSEAAESLGFRTLAVKVSLEKLQKAPLPCIVFWQQRHFVVVYKIGRRYIHV
                     ADPAHGIVKYRHADFVSGWYSDRSNEGEGQGIALLFEPTPALYESEDDFEKSSKPNKK
                     GFGLLWPYLRSHRKLIFQLGLGFLLGSILQLIFPFLAQSMVDMGIHHQNIGFVYTILL
                     AQLMLFLSRITVESIRRWILLHISTRLQISLISDFLMKLMRLPVAFFDAKQTGDLMQR
                     IHDSHRIENFLTVTSLSFVFSIFNLFIFGAIILLYSPFIFLIFTTFSAVSITWITFFM
                     KKRRELDYKRFDRMSENQQGILQIIMGMQEIKLSGSEKQKRWKWESLQAKLFTISRKA
                     LGLGQVQETGTLFINELKNIMITFIAAKETIDGHITLGAMMAIQYMIGQLNAPIDQMI
                     LFLQHAQDAKLSLERLSEIHTHDDEDHESDNSAQLPQERSIRLDRVSFQYEGPRSPYA
                     LKDVNLPIHEGKITAIVGESGSGKTTLLKLLLKFYTPTEGKISVGSDALSQISASFWR
                     RQCGVVMQDGYIFSDTIAHNIAPADETVDYRRLIQALEAANIRTFIESLPLRYNTKIG
                     NEGLGLSQGQKQRILIARAVYKNPQYLFFDEATSALDATNERIIMYNLQRVFQNKTVV
                     IIAHRLSTVRNADQIIVLEKGNVVESGSHIELTQQRGVYFELVRNQLELGN"
     gene            17617..18897
                     /locus_tag="PK937_07920"
     CDS             17617..18897
                     /locus_tag="PK937_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNH32535.1"
                     /translation="MSNDIEPLERIHSDSYDDVMNYEPHWLIRWGSFILLLIIIFLIT
                     VSGYIRYPDILRGQITLISDQPTLEVIAQSAGRVKLFVKDRDTVKVGQPLAMIDNPAD
                     FASIRKLRLFLDSASSQSDSYVMIDTALQLGELQAFYSEVIQNYKELVSFKALNYEVK
                     KINSIRQQINAYYTLGDRLEDQIKLLKLDVTKAEKDFEVNNSLHERGLLSEVELSGYQ
                     FQLNQKKIALEEAQKEAIRNSIVVSDNLKNILDMEFQNQDKTVRYLIRIQESMRKLRS
                     ELSAWEKKYWMTSPVNGEVIYNDVWTDDRFVKLGQEIMTINPKTNRIRGRVKYEGPGL
                     GKVVPGQEVKIKLDNYPFQEFGIVPGIVENISAVSKDKSVVIQITLPQQLKTSFGALL
                     EYKQEMQGTSEIITENRTLLERIFQQLRQITQYQ"
     gene            19239..22820
                     /gene="nifJ"
                     /locus_tag="PK937_07925"
     CDS             19239..22820
                     /gene="nifJ"
                     /locus_tag="PK937_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNH32536.1"
                     /translation="MSNTKRAMVTLDGNEAAAYVAHKLSEVIAIYPITPSSAMGEFSD
                     EWSSKGKANLWGTVPLVMEMQSEGGAAGTVHGALQTGSLTTTFTSSQGLLLMIPNMYK
                     IAGELTSTVFHVAARALASQALSIFGDHSDVMSVRATGWAQLSAHSVQEVMDMALIAH
                     AATLRARVPFIHFFDGFRTSHEVMKVEQLTDEDMLGMIDDHLIRAHRERGLSPEHPVM
                     RGTAQNPDVFFQGREAANPYYQKCPAIVQETMDRFAKLTGRAYKLFDYFGAPDAEHII
                     VAMGSGALTAQETAAFLIGQGHKVGVVKVHLYRPFSIEHLIAAFPKTVRTVAVLDRTK
                     EPGSAGEPLYQDVITALTETFIEGKLPFAMPRVIGGRYGLSSKEFTPAMVKAVYDELQ
                     KPLPKNHFTVGIHDDVTHLSLDVNPDFSIEPQDVVRALFYGLGADGTVGANKNSIKII
                     GEDTDNFAQGYFVYDSKKSGSITVSHLRFGPKPLFSSYLVTRANFIACHQFSFLEKYD
                     MLKYAVEGGTFLLNSPFDENEVWDQLPLIAQKEITEKKLRFFVIDGYRVAKETGMGGR
                     FNTIMQTCFFAISGILPRDKAIEAIKHAIQKTYGKKGDEIVRINFNAVDQTLTNLKEV
                     NYAGKPLTDRAMRPPVDPLAPDFVKDVTGRMILGDGDTLPVSAMPPDGTFPTNTARWE
                     KRNIALEIPVWDPAVCIQCNKCALVCPHATIRAKVFPETALDGKPEHFKAVPYKGKEF
                     PNSLYTIQVAPEDCTGCGLCVDVCPAKNKQETRLKAIYMQPQSPIRDQERIHWEYFLH
                     LPEADRTQVPLNTVKGSQLLQPLFEFSGACSGCGETPYLKLISQLFGDRAMVANATGC
                     SSIYGGNLPTTPWAQNKDGRGPAWSNSLFEDNAEFGLGFRLTVDKQTAFAQELLYRLG
                     DSLDKELIASLIASPQKTEADIQAQRTRVQVLKNRLTALSHPDAKRLLDVADFLVKKS
                     VWIVGGDGWAYDIGYGGLDHVLASGRNVNIIVLDTEVYSNTGGQASKSTNRGAVAKFA
                     ASGKRYSKKDLGLMAMSTGHVYVAQIAMGANDAQTVKALLEAEAYDGPSLIIAYSHCI
                     AHGINMGQGMRQQKSAVDCGHWLLYRFNPDHVSLGQNPLTLDSKAPTLAFKDYALSET
                     RYKMLAMTDPSASESLVKQAQQDILQKWHFYEQMAKLHYNGQNEEKH"
     gene            22870..>23168
                     /locus_tag="PK937_07930"
     CDS             22870..>23168
                     /locus_tag="PK937_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNH32537.1"
                     /translation="MDLTTSYLGMTLKNPIVPSASPFSKDIVQIKRMEEAGAAAVVLH
                     SLFEEQITHDEREMFYHLHHGSESSPEAQSYFVDMDRYNRGPEWYLEHIHRTKAAV"
BASE COUNT         7130 a         4834 c         4598 g         6506 t
ORIGIN      
        1 cagcaattgc gctttatcta tcagaagttc ctcggccggc acatcgtaat cggctttaag
       61 ataatttcgg aatccgtcgg cgaccggttc gaggacggca aaagaagcgg catcggtttg
      121 cgcttgcgta gcgtccgtgc gtcccggcga gaaaggtacc gtgatatttt gctcggcttg
      181 ttttgcagcc aattcgatac cgatacaacc gccaagcacg atcatatccg cgagcgaaat
      241 tttcttcttc cctccgggtt gtgcgcgatt gaattctttt tgaatttttt ccaatttatt
      301 caataccgcg ctcaattgtt ccggttgatt gatcgcccaa tctttttgcg gcgcaagacg
      361 gatgcgtgcc ccattggcgc cgccgcgttt atccgaaccg cgaaatgtag aagccgaagc
      421 ccatgcggtg gataccaact gcgagacggt cagtttggac tcgctgattt ttttcttcag
      481 cgccgcgata tctttttcat taactaattt atgatcagcg gcggggacag ggtcctgcca
      541 gatcagaact tctttgggta cttcttcgcc gagataacgt gaacgcggac ccatgtcgcg
      601 gtgcgtgagt ttgaaccatg cacgcgcaaa agcatcggca aatgcttgcg ggtctttgtg
      661 aaagcgacgt gaaatcggtt cataaatggg atccatgcgc aacgccatat ccgccgtggt
      721 catcatcgtt ggcacacgtt tggtcgcatc gtgtgcagca ggcgcaagat ctttatccgc
      781 tacgtttttt ggtgtccact gccatgcacc ggcagggctc ttgaccaatt cccaatcata
      841 accaaacaac atatcaaagt atccgttgtc ccactgcgtc ggattgggtt tccaggcgcc
      901 ttcgataccg ctggtgatcg tgtcatcgcc tttgccgcta ccatatttac tggtccatcc
      961 aaggccttgt tcttcgatac tcgccgcttc cggttcacgg ccgacatggg aatccggcgc
     1021 cgcgccgtgc gcttttccga acgtatgtcc tcctgcaaca agcgccacag tttcttcgtc
     1081 attcatagcc atacgcgcaa atgtttcgcg aatatcgcgc cccgaagcga ccggatcggg
     1141 tttaccgttg ggtccttgcg gattaacgta gatcaatccc atttgtacag cgccaagagg
     1201 gttttccaaa tcgcgctcgc cactataacg tttatcgccg agccattcgg tctcggaacc
     1261 ccaataaata tcttcttcgg gttcccatat atcttcgcgc ccaccggcaa aaccaaaagt
     1321 tttgaagccc atcgattcta gggcgcaatt accggccaaa atcatcagat cagcccagga
     1381 aattttgttt ccatattttt gtttgatcgg ccacagcaaa cgtcgcgctt tgtccaagtt
     1441 ggcattgtcc ggccagctgt tgagcggtgc aaaacgctgc gtaccgttac cggcgccgcc
     1501 gcgtccgtcc gcaatgcgat acgtaccggc gctatgccag gccatacgaa taaacaaacc
     1561 gccgtaatga ccatagtcgg ccggccacca ctcttgcgaa tccgtcatga gcgcatacaa
     1621 atcttttttg atggctttga gattgagttt tttaaactct ttggcgtaat caaatttttt
     1681 acccatagga ttggacaaag acgaattctg atgcagaatt ttgatattca actggttggg
     1741 ccaccagtcc tgattggttg ttccgcggcc agccgcatgg aacggacact tgctgatttc
     1801 actcataacc tttttctccc tgtttttaga tgaattcgta ggacgatttt aatgttcacg
     1861 atttaaattg gcgaactgat tatatcgagc gtttttatgt cttaaagata cctatttgat
     1921 ttcataagtc aaattgatta ttcttattta ataatagata atatctatat tgaataagaa
     1981 tgacactact gcaactcacc tatattgtcg ctgtcgaaaa gtaccgcagt ttttccaaag
     2041 cggctaaaga tcgttttgtc acacaaccga ctctgagcat gcagattcag aaattggaag
     2101 aagaattagg cgtaacgata tttgaccgag gaaagaaacc cgtagccccg accgacatcg
     2161 gtcgtacgat catcgagcaa gcccaagcga ttttgcgcga aagcgataaa atcatccagt
     2221 ccgtggaatc cgaaaccacc gaagttgcag ggatcttccg catcggtatt atccccacgc
     2281 ttgcgcctta tttgctcaat ctttttctgc aggattttat cacaaaacat ccgcgcgtag
     2341 aattggtcat tgaggaattg cagacgcggg agatcaccga acgattgaac cgaagcgacc
     2401 ttgatgcagg gcttttggct acgccgctgc accataagaa cttagccgaa aaaccgttgt
     2461 tctacgaacc tttcgtcgca tatgtatccg aagaacaccg actaagcgcc aagaaaaaaa
     2521 ttcacatcaa agatctgcaa aacggcgacc tgtggcttct gaccgaaggg cattgttttc
     2581 gcagtcaggc gctcgcacta tgcaaacaaa agaaaaacat aacacctaaa cacaaaggtg
     2641 cattgcgctt cgaaagcggt aatctggata cgcttagaaa attagtggaa caaaattatg
     2701 gtatgacact gttgccgtat ttagccgttt cggaattatc cgatgaaaag aagaaactgt
     2761 acatgcgccc ttttgcgcca ccggctccca aacgggagat cagtttggtg tacaatcgtt
     2821 cttaccctaa aaagaaaatt ctcgaagtgt tagagcacgc catccgcttc tccgtaccgg
     2881 aagaaatatt agaaaaagat agaagtttga tcgtacatca tggtgccgtt tagagaatcc
     2941 attagcgcaa taactcttcc aagagctttt ctttattgac ggttccgata tgcgaccatg
     3001 taagtcgacg gtttttgtat atgtgaaacg tcggcacagt atggattttc aaatcgttcg
     3061 cgatcagcgt atgatcgtca atatttatac taactacgat tatgtcggat ttttctgcaa
     3121 cttcatttac aataggctcc atcaagcggc agggcgagca gtgcgtcgtg taaaaatcaa
     3181 ttaacacggg tctatctgaa gtaatcatgg aatcaaaata actgcgcgac ataccgcccc
     3241 gttcatttaa tgtggtttca gaaaatccct tcgcccgcca attcacaaat ccgccgtcta
     3301 attcataaac acgacggaaa cccatgttgc gcaatttgcg ccgagcgcct gtactgcgtc
     3361 ctccgacgga gcaatacaaa aatatcggct ttcctatatc ccattgcgca gcgtgatagt
     3421 caaatgtccc gacattcgcc caattgacgt tttgggctcc gggaatatgg cctgacatat
     3481 attccttgta cgttcgtaca tcaaccaaaa gagcatcagg attttttttg atactatccg
     3541 caaacgcaga tatcgaaaga tatgctgcgt tttgaaattc ctttttttcc tcacacgatg
     3601 tcagtatgga caatatcata aacatctgaa ataaatcttt ggtgatacga tagatcatag
     3661 ataaaagttt aattccgatt ttatcatcat gtcaatttat atttgacgct cacagaacac
     3721 aagagaaaag acgacttccc aaccaatcta ccgcaatttt tttaaaaaaa actctcaaaa
     3781 tgaacactta atacctacgc attcatgtag tttatttgtg ttagaatttt gacacttaag
     3841 tttttcggga atgtccgatt ttaggtattg atttgcctca ctatgtgtat tattatttta
     3901 ctgtagcaca attcaacaaa tttatctgtt ctcgtcnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnngaat gttctcgtcg aatgttctcg tcgaatgttc tcgtcgaatg
     4081 ttctcgtcga atgttctcgt cgaaacagag gaggaaggga ttggaaaaag agggaactag
     4141 gggtgtcgca gaattggccg agagcgcatg tatgtgcgaa cgccacctcc gccgaaaaac
     4201 gaaagaaaaa ttcggcttac caccgcaccg gcttttagag aatatacgat tggccaaagc
     4261 aatcgaagcc atacatgaac aacctgaagt cgcgctgttt caaatcagtc aaattgccgg
     4321 atatgcatca tacaaatcat tttaccaggc ttttacgagg agattaaaag tgacgccgtc
     4381 ggaagcaaaa cagcatttaa aacaaaatcc aagcgttacg atgtatgctc tacgagagaa
     4441 aatcgaaaaa aaactgacca tataatgagc agtatacgga ttttagttta aagcaattaa
     4501 cgtgttttgt tgattactct caaatatatc attaaacgaa ataagccctt cggtttttgc
     4561 gaaatcattc aataaacgct ttggagtaat tttatattga gtaaaaaatt tgttttaaat
     4621 tttatactaa aaaggagtac tacatgagaa tattcagctt attaataaca ttagtttttt
     4681 tactgatgaa ttgcaaagtt atcgcacaaa atcaaacttt agaacaaagc gtcatggtgg
     4741 atatgataaa ccctagatca ttggatcatc ctatctattt atcattcaaa gacggtgatt
     4801 atcttacatc aattattgaa tgtgataaat gggattctat ttatagaggc acaaattcag
     4861 aagatgcttt tatggaacaa gaagcctatt acaattcttt tgtaggaaat taccaaaaag
     4921 ctcttgaaaa tttcgacaaa attaatgcca aatggattaa tcagtcggat actactaact
     4981 tacttgagtt atcttttttg tcagcaaaaa aggtagatgc tgtaaatgaa attttaagag
     5041 aagcggtaaa ccaccaaatt acgattgtta atgaagcgca tcatatgccg cagcatcgcg
     5101 cctttatatc gtcactactt aaacctttat atgaatccgg ttatcgttat tttgcaacag
     5161 aaatgttaac tgataatgtt gacgctgtga agaaagacgg ttatgttagc tttcaacaga
     5221 acgggtttta tccgaatgaa cccgtatacg ctaacttaat tcgaaaggca cttgaaattg
     5281 gatatacttt agtcagttat gaagatactg tgaactgtga ttttgacccg actaatccac
     5341 ttgcatgttt taatcagcga gaacaaaatc aagctaaaaa catatccaat ttaatcttaa
     5401 agaactattc aaatgcaaaa attcttatac actgcggtta ccagcatgtg gatgaaattg
     5461 aacatcaatc acctaaggtc aaaacaatgg cagcgcattt gaaagagtta agcgattccg
     5521 atcctttgac aattgatcaa actgtaatga gtgaacatgg ctcagttaat tttgaaaact
     5581 tttattacag atacgctcaa gatagcttaa aattaaaatc accaacggta tttaaatcga
     5641 acagtggata ttgggttgat gacactaaac gcggatttgt cgacatgcaa gtttttcacc
     5701 caaggacaaa ttatatcaac ggtagaccaa actggctcat tcaagaaaac cacattctca
     5761 ctttaccgga ttttgatatt tcctcttttc cttttttggt acaagcgttt aaaaaatctg
     5821 acaaaactca ctcaattcca atggatcagt ttttagttaa ttcgagaaaa aaaattgcag
     5881 tagccgtacc caaagggtat tatcggatac gcattattaa caacaaaggc accattcatg
     5941 aactcgaagt aaaaaatcct tagatataat tctaagcctt cacaatttta agacggagac
     6001 aaacaaaaaa agatttgatt aaaatggcgt tgatttgtta cagcgatagt aattttactg
     6061 gtgccgcaaa cttttttatc aaagccacga aaatagacac aacagatggt gggttgtact
     6121 acaacgtagc ttgttgtttt tcgagaattg gaaaaattga taaatctttt gaatggctta
     6181 atgtcgctat ccaaaagggg tacaacgatt accagtggat aaataatgac cccgatttat
     6241 ttaatgtcag acaagatcga cgctgggaag aatttataaa aataaaaaaa tgaaaaggga
     6301 ccaaatgaat attaacatcg cacttatcgg aatgattgta ttgattttgc tagagaccgg
     6361 ttgttctcaa actcattcat ttatgagcga aaaaattgaa gcttgcgggc taagtgattc
     6421 attgatgatt gacgaaaagc aactctttaa aaaaattgat agtctgattg aattctctcg
     6481 ctgtgatact tctgtaatat ttggtatgaa tttattcaaa aagaacgaaa aaaaatctga
     6541 ctggattgct attaagctat atgcgcgaag acccaaacag cgagtcttag atcgcttaga
     6601 gatgttaaaa aaatccgacc aactttacag gaaaaaaatt atagacagta tcggtccaag
     6661 caaaggatgg gcttctgaag aagtgcggca attgtggata aaacaagctg aaattgattc
     6721 tctaaattgg ttaaccttta attccaaaat tatagatcat tatggatggc cgaaaatcag
     6781 tgagtatgga tatgaagctt cagcaacagc atttcttatt gttcaacata caaacgccaa
     6841 aaaccaagag cagaagttgc ctttattgat gaagctagcc gacgagggtg agttttctat
     6901 gtcagagata gcactcttga ttgacaggat aaaagtatct aaaggtgaat ttcaagtata
     6961 cggtagtcaa gtcgaatttg atgcaatatc aaaaaaattt agatttttac caatcgaaga
     7021 cgagaaaaat gtcgatgtaa gacggacaaa agtagggctt ccccccatac aggaatacgc
     7081 gaaaagatgg aacatagatt attcttacat taaataatat tagcgtttaa atcagactta
     7141 ctattttaag caaactatca actcgcgtta gctattcctg tttgccaata gattcagaac
     7201 aatgctgtgg aaattcactt tttatatcaa atatattagt ttgattccag aatcaagcta
     7261 tgtcatgatt accgagttta tataactaac caatcaaaca aactgtcaaa accggcaaaa
     7321 tgtaaatacg atccttacga ggcaaagtgg caaggttgat ttatatttca atatttcttt
     7381 tttttgggtt tacaggatgc aaaactgtaa acatgagttt gtataatgac atcacatgcg
     7441 attattattg tttctattac aatgattctt tgaaaattag tattagattc tttggagatt
     7501 atacttttaa taccccatct gactgggatt cagttaaaac aacaaaactc gcgttacaaa
     7561 ggataaataa aatcaatgat ttctctttat ctgaaagcga catcctcgtt tcgacgcgta
     7621 cagtgattga accttatttt tcttcaataa tatctttaat accatcttca aagattagat
     7681 ttgatcactt aaaatccttc gattctttgg gtacagataa acaagggcac gcgttatatc
     7741 aaaacatatc gccacaagat agtttctgtt tatttcaaaa attgataaag ttaaataatt
     7801 attggctgca cgtaacaggg caaaccatca attcaaccga cggctcgtgt tactcttcgt
     7861 ctttagttga tgaatataat aaaattagca atagcattga atatataaaa cgaagtgaga
     7921 gagctgctct taaaaaccca tatcttattg cggacagctt atttaattct gattctaatg
     7981 gaaactatat caaaccaatt gattacctca aatctgaaaa aaacgtatat gagaattctt
     8041 cactacggaa ttcatatgtc caatgtttgg aaacatacaa gtcgtttgta gggatgacag
     8101 aaccttctct tgtaccaata aatgtctatt ttccaaaccc gaccaattca tttgagccgt
     8161 tctcttcaga gtttggaggt attgttgata ctattatttc tctaacgtct aacaataaaa
     8221 taataatatt taatgaaagt catatttatc catcacaaag gttattgtta tctggattgt
     8281 tgaataagct ttatgaagcg ggattcagaa aaatagcgct cgaggctgta tggaatgata
     8341 aagaaatcaa tgcaattggt tttccaacgc ttaaatcagg gttttacact cgtgaaccac
     8401 aaatggcaaa cctgattcgg aaagcaaaag aaattggata ccaagtcatt tcgtatgaag
     8461 acccaacatt gggaaataga gagtacaatc aggccgttac tttgagtcgt gaattccgga
     8521 aagacagtac agcgaagatg attgtgttat gcgggtcata ccatatacta gaaaagagat
     8581 ctgattcatc tagaacatgg atggctgagc atttaaaaga aatatcaaaa attgatccgc
     8641 ttactttcaa tactgttttt tttgaaaatg tttatttcga taacccgcgt ttactgagtc
     8701 ttatttcggg atccaaactc aatgaaatag ctccagttgg ttttaatttt gttaacgatt
     8761 attacctatt taatgttacg agtttaaaac cctttacctg tgataatgag aaaggtaact
     8821 tcgcccctta taaacccatt caaatcgttg ttcctcaatt gattaaaaaa gtactattag
     8881 tgcaaatatt ttcaagcaag gaatacggca tacgcggcag agtacctgtt gcttcacatt
     8941 atgttgatag cgagaactgt caaatgacat attggcttcc tccggatgag tatgtcgttg
     9001 tgtattacgg ggcatctggt gaaatcatat tagttaataa aatcatctta agttaggagt
     9061 taagtttgta cagaattatg atgtataaag acagtaataa ttacatagtt tcttattttt
     9121 ttaaatttga aaactattag gctttcaatt ggaaagcgct ataaaaaaac gtgcgaggtt
     9181 acttgacata atctactaat ctaaaaaaca atcacccttc ttttttgtta tcccttaccc
     9241 gtttttcccc ggttcataac gacataaccc caacgatttc attgttcgac aaccgacaaa
     9301 agaggtttca gaagttgtgg catataacca aagacgatat tcacgtttgc aaagattgtg
     9361 agtttagata tatgtgccac gattgtcgag catatactat tggagggctt caggaatcaa
     9421 agccacagaa atgccgatat aatccttatg atgcgcaatg gaacgatata aaatcttcaa
     9481 aagtattaca tggccataag taatgcaggg gaagtgatgg aaaaccctaa aatagagtac
     9541 atcagaaatg taacgaggta ttacacatga aaaccaaaga aaaaatggaa gtgatcgatt
     9601 cgccgctttt tcaaactatt gaaagagaac agatgaaaag cgttcaagga ggcaagccac
     9661 ctggttggac aatggcagaa cagactgtgt actctaacgg gacatcatct gaagatagcg
     9721 cggcgggaaa tgattaatga atcatcaagt aaagatgggg tatcatgccc catctttaca
     9781 cttttctatt aaactcataa tgttcaaatt aaattgcaag aatggtatta attcttacat
     9841 acgacggaga tacaacttca gattatataa tcgaagtatt aaaaaacaaa aaacatccat
     9901 atttaaggtt aaactcatct ctttttttta aagaaaaaat cactatcgcc cctttacata
     9961 aatttattga aattaatgga ttcaaatata catccgattt gttgagagtg atttggttta
    10021 ggcgatttta ttcttataga aatttaaaat tcatttatcc caagagaatt gttaataagt
    10081 cagattttcc aaacttaaca ttcgagttta ataaaataat taggacatta tacgcgactc
    10141 tgaatagttc tcattggcta cttgacccta gacatgcctt tgtaaataaa atagaggttt
    10201 tacaaacggc aaaggaaata ggacttttgg ttccaaatac aactatcatt aataacaagg
    10261 cctttttatc caaaaaagaa aactcgaact tagtttcaaa atcaatagct gagggagttg
    10321 gtgtaaaagc taagagatat aatttttcaa tgtacacaaa aactatttca cctgacttta
    10381 tcagcaaact tccatcatta tatgtacctt ctttggtaca agagagaatt aaaaaacgtt
    10441 acgatataag aattttttat ttaaatggta ggatatttgc ttctgctatt ttctctcaga
    10501 acaacgagcg tacaattgat gactttcgca tatatgacga taatcgaccc aatagaattg
    10561 ttccttataa attacctaat gcaattaaaa gaggaatccg tcgcttaatg aaaaaactat
    10621 cgttaaacat tggagcgatt gacatggtac gatcccaaac aggggaatat tacttcttag
    10681 aagttaaccc taatgggcaa tttggaatga tttcgcatcc atgtaactac aatctattcg
    10741 aggtcgttgc tgattatttg ataagaaatg acaagtaaaa caaaaatatt cctatcaata
    10801 cacttgttca tgttattagt cgggtgttct atcccaacta atcaatttat ttcacacaat
    10861 cgatttcaaa aaacgatggg aagagttgtt tatcacaatg acagcttgag attatcggcg
    10921 atgttttatg gagattatgt ttttcattca ggcaacaagc aacgtgagat aagtagaaaa
    10981 tctatagaaa tcataaagag cggatataaa gagggattat acctaacaga gaaagattta
    11041 atttttacag ctgaaacgat cgtggaaccc aaattttatt cagcgttgtt tagtttacat
    11101 aacctcagca aaagacgtct aaatgaaata agtaaaattg ctcccgactt tttctggagt
    11161 gattccgttc gtaatgtcgc aggtattggg caaattttta ctgatgctac atctgcagtg
    11221 tttttgattg gttttactta tgatcatttg actgttagaa ataaaaattc tttacaacag
    11281 caatttttct tcaaccaaac tttaaatgca ttatcacgcg agtttagaga cgctgacgag
    11341 tccattttta aatctgtact cagcggaagc aattataaat tgtttgaggc tgaagaccca
    11401 tttattttag ctaataaata ttatcaaact gaatattcgg gaaattatct tctacctatt
    11461 aatgaactac tggaatcaga accgaactat gtcagtacgc aagatcgata taaatattta
    11521 caagcgctat tgacctatat gtcttttatt gatcaccccc aatatgatta ttacaataaa
    11581 aaaagaaagc aaatacagtc aagatcaatc atcaattatt ctaaacgttt taacttaaat
    11641 attgatgaaa tacaaaaaac cattttagac tcttcctcgc atcagcgcgt tgtcatgttt
    11701 aatgagtctc actatgtacc gtcaattcga tcttttatta tgactttgct agacggtcta
    11761 tatcaacaag ggttccgtta tttggcgttg gaagcattgg aagaagatcg ccttgttaat
    11821 gaaaaaaagt ttcctgtcat ttcttcaggt ttttattttc gagatttccg catggcaaat
    11881 atcgcgcgta tggctaagaa attggggtac catttgataa aatatgattc gcgagaaacc
    11941 aacagagagg aaaagcaggc gcagcgttta gcacagctaa tagctaacag cgataccaat
    12001 aaaattgtag tattatccgg tttttcacat atagacgaaa atcctgataa aaagagaatg
    12061 gcttcgatct taaaaaaaat ttccggcata aaccccttta ccatcaatat gactgatatt
    12121 tctgaaacca gaattgaatt gaattctaat atttgtttta caaataactc tacagatagg
    12181 atacagttac catcatattt tatgaacaac gacttatacg tatctatttg taatgacgac
    12241 ttcataaacg acgcttttac ttttcataaa catgaaacgt taaatatcca tctggatttt
    12301 tccggtatcg ccaagcgcgg cgaatatgtt ttgcttgtgt ttgatatatc ggagtctcca
    12361 aacgaagggc aattacccgt ttttgccaag ctcgttctcc caaaaatgca tgagcaaatt
    12421 gatataaaac tattgcctgg acgctataat gttttactaa aatcagaaga tggattaatt
    12481 tgtcacgata ctactttata cgttgaagta aataaataac gatttaggat ttcgatacgc
    12541 ccatattcaa catgtgaact ttcacaaact atttcttaca aatttgcaga aggaagcgtt
    12601 tttcacagaa tcacctattg aagaaaagaa acctgtaatc agtttacaga tttagctgga
    12661 gtggggtatc aaattttacg tctaatcaac catgaactgt tctaaacatc ttaattataa
    12721 acgctcctcg cgcaaaacaa tagtgcttca ttggttatta agtggccact tttttttata
    12781 tcacattaac ctttggtcag aaagtagtta atcaacaaga cccatcgagt atttgtattc
    12841 gctttaaagt taaggtagat acaaatgaac ctgatttaaa aaagaaatcc atactatcca
    12901 aaattattta aacgtatatc ctgaaaatgg tttcgttttt tttggagtta taaagagcga
    12961 tatcctcatg aaattgcact gcaatctgtg gtattatgcc cagtaagctg ttgatcgagg
    13021 ggctatccac atgacttggc ggaggtcaca acaaaaactt tactttaaat atttagtaga
    13081 aaaaattcga cctaaacttc aatctcagga cagtttgttt gttgcgaaaa ctatctccgg
    13141 tccttatgga aatgtggaag aatacaatac ggtaggaggt attattagcc atcttctctt
    13201 tggaaaaaaa gacgatgcaa atttcatttc ttttttgaaa taaataaaag aactgtttac
    13261 tttgtttgaa aaggaatttg gcattaaaaa gtattattgg tgaaatttta tctgatgaaa
    13321 cggataaaat atcttcgaac taaccgtttt agttttgatc ttattttaaa gaaaaagaag
    13381 gagctacact acactgtcag atcaagttga atcaaatgat gatagtataa gaagcttaat
    13441 gttacccaat ggtacatagg aattaagagt tatagatttc aaaggtaaaa ttatagaaaa
    13501 aacgattgta gaggtaaaat gagtaagaag ttctcattag ctttggtttt ctatgtttgt
    13561 tttgcaactg taaaatccca agatattaaa aacattgatg aacaagctac agcattgtat
    13621 ttaaaaaaag aatatttgaa atgtgctgaa acatttcaaa aagcaatcgc cgcagattca
    13681 aattacttta gcgcttatta taatacggca tgctgctaca ctttggctgg caaatttgaa
    13741 ttgtcaatta aatttttaaa acaggctatt gatcgcggat tttctaacga gcgcatgcta
    13801 aaaacagatc cagacttaaa tcaactgaga agccgcgacg aatggaaaga aattttagag
    13861 tattttcata aagtgcgata ctgaaatata tttcgagtaa gatttccgag tttttttcaa
    13921 tacaataaaa ttataactct gtgtaaatcc ggatgaaaac gatggcatta taccctctaa
    13981 acaagttcta atacacctaa catgtataga gggttgtatt tctttctgac cgaagctata
    14041 aactacaaaa tatactcatg acccgtttgc gggcgagttg ccaaatcaaa caaacactat
    14101 gaagaacatg aagtcgttcg caatacttat cctgctttca ttaaaccaaa tatatgcgca
    14161 gtattctctc ccaccggcaa tcatggattc gattcgggat attgataggt cactatcttc
    14221 tgatcgtaac tatatagccg ctttgactcg gttaaaccga attcatgcaa tgccacaata
    14281 tgccgggcag cctatattgt ttaatcattt gggcttgtat aattctttag tgtccgatta
    14341 ttcgacggcg gttgcttttt ttgactccat tgagcttctt cgtcagagtc aacgccatac
    14401 cacatcattg ccgccaaccg gtttaacctt tgatacaagc caaatacgcc cctttcccgc
    14461 gatggaaacc atacttcgcc ttgcaaagaa taattccgtc gtaatgatca atgagcgtca
    14521 tcacatgcct atgcatcggg cttttatttt ggaacttctg attcgcctca aagcgctagg
    14581 atatacccac tatgctgccg aaggattgca aaatcacgat acaatcacaa gccctgcaac
    14641 ataccctgcg ataggaaaga caggatatta tgtaaatgag tcggtatttg gtgaattgat
    14701 acgaactgcc attctgttgg gttataagat agtcgcctat gaaacaacaa gctcggcatc
    14761 tcccgaatcc gatcctatca aaaaccaaaa taaaagggaa aaagaacagg ctgaaaattt
    14821 gtacaataaa actttgcgcc aagaccctca ttgtaaagtt ttagtccatg tcggttatgg
    14881 tcacaacgat aaaatgggag ttggcggctg gatacctatg gcatcctact tcaaaactat
    14941 aagcggcgtc gagcctttgt ccattgatca aattgaaatg tttgaacacc gtgttccgta
    15001 ttttgaaaat ccctattacc ggtttatatc cggtatgatt tcgcagccat cggtttttat
    15061 tgaaaactct tccaatacat tttggtcgta cctacaacga ggtagttacg atctggaaat
    15121 atgccacccc aaagaaactt atgttcatgg acgcccgaca tggagaaaca tagctaaccg
    15181 ctatcggatg tatgacctac ccattagtaa aaaaacaatt gcaaaaggat tgttacaggc
    15241 gttttatcat gatgaacctt cggatgctgt tccagcagat cagatcgttt ttaataccgg
    15301 cgttcaacag atgattcctt gtttattctt acagccagga aaataccggt tcaaattagt
    15361 gagtgaggat agcaccataa gcacggaatg ggttgatata atcaaataaa catgaacagc
    15421 tttcccatat atcttcagca tgacaaaatg gactgcggcc ctgcttgtat ccgtatgata
    15481 acaaaatttt ttggacgtac agtcagcccg gaaaaattac gccgcatcac ctatgcctcc
    15541 aagggaggca cctctctttt gaatttgtcg gaagccgcgg aatctttggg ttttagaaca
    15601 ctagcggtca aggtatctct cgaaaagctt cagaaagcgc ctctgccttg tattgtgttt
    15661 tggcagcagc gccactttgt cgtagtatat aagatcggtc gaaggtatat ccatgttgcc
    15721 gatccggcgc acgggattgt aaaataccgt cacgcggatt ttgtatccgg atggtatagt
    15781 gaccgatcca atgaaggtga gggtcagggt atcgccctgc tcttcgaacc tacgccggca
    15841 ttgtacgaat cggaggatga ttttgaaaaa tcgtcgaaac ccaataaaaa gggctttggc
    15901 cttttatggc cgtatttgcg atcgcatcgc aagctgatat ttcagctcgg tttagggttt
    15961 ttattgggaa gcatattaca gctcatattt ccgttcctcg cccaatctat ggtagatatg
    16021 ggtattcacc atcagaatat cggttttgta tataccatac ttttagcgca actgatgctt
    16081 tttctcagtc ggatcactgt cgaaagtatc cggcgatgga tactcctgca tatcagcacg
    16141 cggctacaaa tatctcttat atccgacttt ctgatgaagc tgatgcgttt gcctgtggct
    16201 ttttttgacg ccaaacaaac cggcgatctg atgcaacgca tccatgacag ccatcggata
    16261 gaaaactttc tgacagtgac ttcgctcagt tttgtttttt ctattttcaa tctttttatc
    16321 ttcggcgcca tcatactttt atacagccct ttcatatttc tgatttttac aactttcagc
    16381 gccgtttcga taacatggat cacgtttttt atgaaaaaaa gacgtgaact ggattacaaa
    16441 cgatttgatc gtatgtccga aaaccagcaa ggcatactcc agatcatcat gggtatgcag
    16501 gaaatcaaac tcagcggctc ggaaaagcag aaacgatgga aatgggaatc cctacaagcc
    16561 aaacttttta ccattagccg taaagcgctg ggtttggggc aagtacagga gaccggtacg
    16621 ctttttatca atgaacttaa aaatattatg atcacattca tcgccgccaa agaaacaata
    16681 gacggacata tcacgctcgg tgcgatgatg gcgatacagt atatgatcgg tcaactgaat
    16741 gcacctattg atcagatgat cctttttttg caacatgccc aagatgccaa actgagttta
    16801 gaacgattat cggaaataca tacccacgac gatgaagacc atgagagcga taacagcgct
    16861 caactgcctc aggaacgatc cataagattg gatcgggttt catttcagta tgaaggtcct
    16921 cgctcgccct acgcgctcaa agatgttaac ctcccgatac acgaaggaaa aatcacggct
    16981 atcgtcggtg aaagcggtag cggtaaaacg acattactta aacttttgct taagttttat
    17041 accccgacgg aaggaaaaat aagcgtcgga tcggatgcac tgtcgcagat cagcgcctct
    17101 ttttggcgcc ggcagtgcgg tgtcgtcatg caagacggat atattttttc ggatacgatc
    17161 gctcacaata tcgctccggc ggatgaaacc gtagattacc gaaggctaat acaagccctc
    17221 gaggccgcta atattcgaac attcatcgaa tctttacctc tacgatacaa taccaaaatc
    17281 ggaaatgaag gactcggact cagtcagggc caaaagcaac gcattcttat cgctcgcgca
    17341 gtttataaaa acccgcaata tttgtttttt gatgaagcta ccagcgcgct ggacgctact
    17401 aacgaacgga ttattatgta caatctccag cgcgtcttcc aaaacaaaac cgtcgtcatc
    17461 atcgcgcacc gactcagtac tgttcgcaac gccgatcaga ttatcgtact agaaaaagga
    17521 aacgtggtag aatccggttc gcatatagaa ctgacccaac aacgaggcgt ttacttcgag
    17581 ctggtacgca accaattaga actcgggaat tagaagatgt ccaacgatat cgaacccctt
    17641 gaacgtattc acagcgacag ctatgacgac gtgatgaatt acgagccgca ttggttgata
    17701 cggtggggct cgtttatatt gctattgatc attatttttt tgattacggt cagcggatat
    17761 atacgatacc cggatatatt acgcggacaa atcacgctta tcagcgatca acccacattg
    17821 gaggttatcg cacagtccgc aggccgcgtg aaactgtttg tcaaagatcg cgacacggtc
    17881 aaagttggcc aacctttggc tatgatcgac aatcccgccg actttgcatc cattcggaaa
    17941 ttgcgcttat tcttagattc cgcatcatct cagagtgata gttatgtaat gatagatacg
    18001 gcgttacagc tcggagaact gcaggctttt tattcggaag tgatacaaaa ttataaagaa
    18061 ttagtttcgt ttaaagcgct caattatgaa gtaaaaaaga ttaactccat tcggcaacag
    18121 atcaacgcct attatacttt gggcgaccga ctagaagatc agatcaaact tctgaagttg
    18181 gatgtgacaa aagcggaaaa agattttgaa gtcaataatt ctttacatga acgcggactt
    18241 ctgtcggagg ttgaactttc cggttatcaa tttcagttaa atcaaaaaaa aatagcgcta
    18301 gaggaagccc aaaaagaggc cattcgcaat tcgatcgtgg tttctgataa tttaaaaaac
    18361 atacttgata tggaatttca aaatcaggat aaaaccgtac gatatttgat ccgcatacaa
    18421 gaatctatga gaaaattgag aagcgaatta tcagcatggg aaaaaaaata ctggatgacc
    18481 agtcctgtca acggcgaagt catctataat gatgtatgga cagacgaccg atttgtaaaa
    18541 ctgggccaag aaatcatgac gatcaatccg aagacaaatc gtattcgtgg acgcgtaaaa
    18601 tatgaaggtc ctgggctcgg aaaagttgtt cccggacagg aagttaaaat caaattagat
    18661 aattaccctt ttcaagaatt tggcatcgta ccaggcatcg tagaaaatat ttcagccgta
    18721 tccaaagata aatccgtcgt catacaaatc acgctcccac aacaattaaa aacatctttc
    18781 ggcgccttgt tggagtacaa acaagaaatg caaggaacat ccgagattat caccgaaaac
    18841 cgcacgttgc tcgaaaggat ttttcaacaa ttgcgccaaa ttacccaata tcagtaaatt
    18901 aatttgtatt tgctcgtggt ttccaaatac atgacttctt atttttttaa atttgaaaac
    18961 tattaggctt ccaattggaa agcgctataa aaaaacgtgc gaggttactt gatataatct
    19021 actaatctaa aaaacaatca cccttctttt ttgttatccc ttacccgttt ttccccggtt
    19081 cataacgaca taaccccaac gatttcattg ttcgacaacc gacaaaagtc attttttgcg
    19141 atgatacgaa tcattccaaa gtaatcggat ttatgataga ctgctcttag tcgggtgctt
    19201 tgtgcacggc cggctattgt ccaaccaacg aggctgttat gtccaacacc aaacgcgcga
    19261 tggttacgct ggacggcaat gaagccgccg cttacgtagc ccacaaactc agcgaagtca
    19321 tagccatcta ccccatcacg ccgtcttcgg cgatgggcga attttccgat gagtggtcca
    19381 gcaaaggaaa agccaatctt tggggaaccg tgccgctggt catggaaatg cagagcgaag
    19441 gcggtgcggc gggaactgtg cacggcgcat tgcaaaccgg ttcactcacg acgacattta
    19501 cttcatcgca gggactcctg ctgatgattc ccaatatgta taaaatcgcc ggcgaactca
    19561 cctcgacggt atttcacgtc gcggcgcggg cattggcttc acaagcgctt tcgatattcg
    19621 gcgatcactc cgacgtgatg tccgttcgcg ctacaggatg ggcacaactg agtgcacatt
    19681 ccgtacagga agtgatggat atggcgctca tcgcgcatgc ggccacgctc cgcgcacggg
    19741 taccgttcat tcattttttt gacggatttc gcacgtcgca cgaagtcatg aaagtagaac
    19801 aacttaccga cgaagacatg ctcggtatga tcgatgatca cctcatccgt gcgcatcgcg
    19861 agcgcggtct ttcaccggaa catcccgtca tgcggggcac cgcacaaaat ccggacgtat
    19921 tttttcaagg gcgtgaagcc gccaatccct attatcaaaa atgtcccgcg atcgttcagg
    19981 aaactatgga tcgttttgcg aagctcacgg gtcgcgccta caaattattt gattatttcg
    20041 gcgctccgga tgcggaacat attatcgtcg cgatgggttc gggcgctttg acggcacagg
    20101 aaaccgccgc attcctgatc gggcaaggcc ataaagtggg cgtagtcaaa gttcacctct
    20161 atcgcccgtt ttcgatcgaa catcttatcg cggcttttcc taaaaccgtt cgcaccgtcg
    20221 ccgtgctgga tcgcaccaaa gaaccgggca gcgcaggtga accgctgtat caggatgtca
    20281 tcacggcgct tacggaaact tttatcgaag gaaaattgcc cttcgccatg ccccgtgtga
    20341 tcggcggccg ctacggctta tcatccaaag agtttacgcc ggccatggtc aaagccgttt
    20401 acgatgaatt gcaaaaacct cttcctaaaa atcattttac cgtaggcatt catgatgacg
    20461 taacgcattt gagcctggat gtgaatcccg atttttcgat cgaaccccaa gatgtcgtgc
    20521 gggcattgtt ttacggactc ggcgccgacg gcacggtcgg tgccaataaa aactctatca
    20581 aaatcatcgg cgaggatacc gataatttcg cccagggtta ttttgtgtac gattctaaaa
    20641 aatccgggtc tatcaccgtt tcccatttac gattcggccc gaagcctctg ttttcctcgt
    20701 atctggtgac ccgtgccaat tttatcgcgt gccatcagtt tagttttctt gaaaaatacg
    20761 atatgctcaa atatgccgtc gaaggcggta cgtttttact caatagcccc tttgacgaaa
    20821 atgaagtgtg ggatcaattg ccgctcatcg cacaaaaaga aattacggaa aaaaaattac
    20881 gcttttttgt gattgacggt tatcgtgtgg ccaaagaaac cggcatgggc ggacgattta
    20941 atactatcat gcaaacttgc ttttttgcca tatccggcat tctcccgcgc gataaagcga
    21001 tcgaagcgat caaacacgcc attcaaaaaa cctacggtaa aaaaggcgat gaaatcgtac
    21061 gcatcaactt taacgccgta gatcagactt tgaccaatct caaagaagtc aactatgccg
    21121 gaaaaccttt gaccgatcgt gcgatgcggc ctccggtgga tcctctggcg ccggacttcg
    21181 tcaaagacgt aaccggccgt atgatactag gcgacggcga cacattgccg gtaagcgcca
    21241 tgccgcccga cggaacattc ccgaccaata ccgcacgttg ggaaaaaaga aatatcgccc
    21301 tcgaaatccc ggtctgggat cccgccgtgt gtatccaatg taacaagtgc gcattggtat
    21361 gcccgcacgc aacgatccgc gctaaagtgt ttcccgaaac tgcactcgac ggtaaaccgg
    21421 aacatttcaa agccgtacca tataaaggta aagaatttcc caacagcctt tataccatcc
    21481 aagtggcccc tgaagactgc accggctgcg gcttgtgtgt cgatgtatgc ccggcaaaaa
    21541 acaaacagga aacccgcctc aaagcgatct atatgcagcc gcaaagcccg attcgcgatc
    21601 aagaacgcat ccattgggaa tatttccttc acctcccgga agccgatcgt acacaggtgc
    21661 cgctcaatac cgtcaaaggt tcccagttat tacaaccgct attcgaattt tccggtgcat
    21721 gcagcggctg cggcgaaacg ccttacctca agctgatcag tcagcttttc ggcgatcgcg
    21781 caatggtcgc caatgcgacc ggctgctcta gcatctacgg cggtaatctg cctacgacac
    21841 cttgggcgca aaataaagac ggacgcggcc cggcgtggag caattcgctg ttcgaagata
    21901 atgccgaatt tggtctcggt ttccgtctca cggtggacaa acaaacagcg tttgcgcaag
    21961 aactgctcta tcgcctcggc gactcattgg acaaagaact tatcgcatca ttgatcgcgt
    22021 caccgcaaaa aaccgaagcc gatatccaag cgcaacgcac acgcgttcaa gttctgaaaa
    22081 accgccttac cgcattatca catcccgatg ccaaacgttt actcgacgtt gccgattttc
    22141 ttgtcaaaaa aagcgtctgg attgtcggcg gcgacggctg ggcgtacgac atcggttacg
    22201 gtggcttgga tcacgtactc gcatccggac gcaacgtcaa tatcatcgtg ctggacaccg
    22261 aagtgtattc caacaccggc ggccaggcgt ctaaatccac caatcgcggc gcggtagcta
    22321 agtttgccgc ctcgggaaaa cgttattcca aaaaagatct cggcctcatg gcgatgagca
    22381 ccggccatgt ttatgtcgca cagatcgcga tgggcgccaa cgatgcacag actgtcaaag
    22441 cgctattgga agccgaagct tacgacggac cttcactcat catcgcctat agccactgta
    22501 tcgcacacgg tattaatatg ggccagggta tgcgccagca aaaatccgcc gtcgattgcg
    22561 gtcattggtt attgtaccgt ttcaatccgg atcatgtgtc tctgggtcaa aaccctctca
    22621 cattggattc aaaagcgcct acgcttgcat tcaaagatta tgctctgagc gaaacgcgtt
    22681 ataaaatgct ggccatgacg gatccatcgg catcggagag tttggtcaaa caagcgcaac
    22741 aggacatact tcaaaaatgg cacttctacg aacaaatggc gaaattgcat tacaacggac
    22801 aaaacgaaga gaaacattag acgtgaattc agaatttcgc attccacaaa attttcttga
    22861 ggacgcacta tggacttaac caccagctat ctcggaatga cgctcaaaaa cccgatcgtt
    22921 ccttccgctt cgcctttctc caaagacatc gtgcaaatca aacgcatgga agaggccggc
    22981 gccgccgcgg tggttttaca ttcgttattc gaagagcaaa tcactcatga tgaaagagag
    23041 atgttttatc atttgcacca cggatcggaa agttcgcccg aagcgcaatc ctattttgtg
    23101 gatatggatc gctacaaccg cggacccgaa tggtatctgg agcatattca tcgcaccaaa
    23161 gccgccgt
//
LOCUS       DAOQGF010000045        22988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1029_length_22988_cov_7.160162, whole genome shotgun sequence.
ACCESSION   DAOQGF010000045 DAOQGF010000000
VERSION     DAOQGF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_22988_cov_7.160162"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..584)
                     /gene="atpC"
                     /locus_tag="PK937_07935"
     CDS             complement(168..584)
                     /gene="atpC"
                     /locus_tag="PK937_07935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNH32538.1"
                     /translation="MDNNKYHLEIISPRHVVFKGEVESFTAPGASGSFQILKNHTAYI
                     SSVLIGEVKIRHTDGYDEIYATSGGFVEVEHNKVTFLAETCEKVSSIDLIRAQESYER
                     ARKRLEDRSSYDPERSRQALLRSRNRLRLAEKYNKQ"
     gene            complement(606..2003)
                     /gene="atpD"
                     /locus_tag="PK937_07940"
     CDS             complement(606..2003)
                     /gene="atpD"
                     /locus_tag="PK937_07940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNH32539.1"
                     /translation="MNKGKIVQIIGPVVDIEFANGQLPEIYNSAHVTRPDGSTLVVEV
                     QQHLGENRVRAVAMDATEGLVRGMDAVDTGAPIAVPVGEKVLGRLLNITGDTIDGFGK
                     IEASNKRMPIHRPAPKLEELTTKSEMFETGIKVIDLIEPYMRGGKTGLFGGAGVGKTV
                     LIQELIHNIAKQHGGYSVFAGVGERTREGNDLYHEMKDSGVLNKTALVFGQMNEPPGA
                     RQRVALTALTMAEFFRDDEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPNLA
                     TEMGELQERITSTKKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRAISELGI
                     YPAVDPLDSTSRILDPLVVGQEHYDTAKRVQEILQKYKDLQDIIAILGMDELSDDDKI
                     VVARARKIQRFLSQPFHVAEQFTGTPGRYVKKEDTIAGFKAIAEGQMDDLPEQAFYMV
                     GTIDEAEEKAEKMRR"
     gene            complement(2036..3127)
                     /locus_tag="PK937_07945"
     CDS             complement(2036..3127)
                     /locus_tag="PK937_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32540.1"
                     /translation="MTIRYVFLVATMVTRLTAGEENAVGFTFLGIRPDARSAAIGPMV
                     AIPNAWSAFYNPAGIAFASGLGMSYNTNDYVANTSINYWTAGMGHTRFGSLAVNNYRF
                     NYPRIERTNEENPDGTGEYYRPYENAFSASYAYAWSGWLAVGISHKWIYSKKDLFTAK
                     AQFNDIGIMARLPLVWFNTGGYRNEISAGFSSINNRAGKKPVYKSDYHETIVDPMSND
                     TSFVFFGGDPIKVQRFDYWAFAYGLSYQSDAWRFTVFKGMIQLVYKDANVLDDELRVG
                     TEWTIFDILSLRTQTKNFEKQKNKPRYKEKLIGGAYGASVIIPLKVLDISQWDITPRI
                     DWTRSRGLYEKQTYTYWNFGIQMNFWNTK"
     gene            complement(3169..4056)
                     /gene="atpG"
                     /locus_tag="PK937_07950"
     CDS             complement(3169..4056)
                     /gene="atpG"
                     /locus_tag="PK937_07950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNH32541.1"
                     /translation="MATLREIKRRIVSVKSTQKITKAMKMVAAAKLRRAQENIISARP
                     YAYKLGALIKHLARNEEVQQNPLIKPRDVKNIMIVVVTADRSLCGAFNTNIIRYVNNL
                     LKGDLKSKLDNNNVKLVTVGRKGDEFFTKRHFPVVFRHANFFNHLNFDDASKVITYCV
                     QEYSAGRVDEVHVVYNEFKSAIQQNLVMERLLPVEPEKVESSNVKYNIETDYIYEPSQ
                     YEIINTLIPRHLNVQMWRILLESFAAEMGARMTAMESATENAGDLIRTLTLYYNRARQ
                     AAITKEILEVVSGAEALRK"
     gene            complement(4066..4425)
                     /locus_tag="PK937_07955"
     CDS             complement(4066..4425)
                     /locus_tag="PK937_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32542.1"
                     /translation="MKRIARIITLICIGAALYGCAPKAQEVPMETFVDIWIQAQSDSA
                     FKAKYAKPFEEATAADLEAFAKPYGYTGADMKFTTSEIISKNKNKENEFLERMNEATL
                     RTLNEKPDTSAVIESKK"
     gene            complement(4422..4766)
                     /locus_tag="PK937_07960"
     CDS             complement(4422..4766)
                     /locus_tag="PK937_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32543.1"
                     /translation="MRNVLLLFGWVMLAGCGPKEIPAEPMATITYEVMRSKSFLVDDV
                     KFEELPDADLEPIVKKLGFTPSDYRHTVKLFDNDAAKKNDLAVRIGKLLQSDLEKASQ
                     NDSTVFKQLMTK"
     gene            complement(4779..6305)
                     /gene="atpA"
                     /locus_tag="PK937_07965"
     CDS             complement(4779..6305)
                     /gene="atpA"
                     /locus_tag="PK937_07965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNH32544.1"
                     /translation="MAVSVRPDEVSGILKKQLASFEREMDTYETGTVLQVGDGIARIY
                     GLTKAMASELVEFPNGVVGMLLNLEEDNVGAVLFGDSSGIKEGDTVKRTGRLAEVAVG
                     PEMLGRVVNALGQAIDGKGAINAKQKTLIERKALGVIERQSVKEPLATGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAVAIDTIINQKGKDVFCIYVGIGQKESTIAKVVKILEDNG
                     AMAYTTVVSAAASEPAPLQFLAPYTGCAIGEHYRDNGKHALVIYDDLSKHAVAYRQMS
                     LLLRRPPGREAYPGDVFYLHSRLLERAAKLNQDLGGGSLTALPIIETQASDVSAYIPT
                     NVISITDGQIFLESNLFYSGVRPAINVGISVSRVGGSAQIKAMKQVAGRLRLELAQYR
                     ELEAFAKFGSDLDKATQQQLRRGARLVEILKQGQYQPMSFDAQITIIYAATNGFLDDV
                     PVGDCRRFEKELTETMELRHKDVLSAIMEKKELTDDIKNKLNTIIGDFKAAFKVTKED
                     "
     gene            complement(6369..6917)
                     /gene="atpH"
                     /locus_tag="PK937_07970"
     CDS             complement(6369..6917)
                     /gene="atpH"
                     /locus_tag="PK937_07970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNH32545.1"
                     /translation="MKNSATARRYAKALFDISVEQNALDAVYADMQSIATMIEQSPEF
                     RAFLHNPVIPNAQKKDAMKALFDKKIHTEVYRFMHLICDHDRENILGDMIEAFETLRN
                     EKLGFIHAEVISVVDMTPDQVKGVTEKIKALTGKTPQLVFKKDASLIGGFMVRIGDTM
                     IDGTVKHQLERLKSAFTAVYHH"
     gene            complement(6957..7505)
                     /gene="atpF"
                     /locus_tag="PK937_07975"
     CDS             complement(6957..7505)
                     /gene="atpF"
                     /locus_tag="PK937_07975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNH32546.1"
                     /translation="MKTLLLLHMISGGGEHKGGLLDIDPGLIFWTIVTFVILLVLLRL
                     TAWGPIIKMLEEREEKIKNSLEEAEKARREAAALTEKNQEIMAKAEREAQEVARRAKE
                     NAEKLKTEIMDQAKAEAARLVAEARTQIENEMNSAISALRKEVGDMAVTAAGKILATN
                     LDADKHKKLIDDFIKDMPVGRN"
     gene            complement(7611..7838)
                     /gene="atpE"
                     /locus_tag="PK937_07980"
     CDS             complement(7611..7838)
                     /gene="atpE"
                     /locus_tag="PK937_07980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNH32547.1"
                     /translation="MGDLGFGFLAAGIGAGLATIGAGLGIGKLAASALEGSARQPESA
                     GDIRTTMIIAAALIEGVALFAEVICILLALK"
     gene            complement(7911..8816)
                     /gene="atpB"
                     /locus_tag="PK937_07985"
     CDS             complement(7911..8816)
                     /gene="atpB"
                     /locus_tag="PK937_07985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNH32548.1"
                     /translation="MPMLTFWQAASQEAPKAADKAEKVDIGGTIIHHITNSNEIELPI
                     LGYVKLPHFDPIHIGTMTIDLSPTKHVVMIWLVALLLLVIFSILARPKLIPRGLYNAM
                     EALISFVREEIVRPNFGAKTDRYIHYFLTLFFFILFMNLLGLIPYGSTATGNIAVTAT
                     LAVVTFFVTQYTAIKSQGIGGYLKHLTAGVHWLLWPIMIPVEVVGLFTKPFALSVRLF
                     ANMTAGHVVILSLLGLIFVLKTYFVAPISILFALFIYVLELFVAFLQAYIFTLLSSLF
                     ISMGMHHEHHEEHHDAEHPVHAGHH"
     gene            complement(8836..9225)
                     /locus_tag="PK937_07990"
     CDS             complement(8836..9225)
                     /locus_tag="PK937_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32549.1"
                     /translation="MSQKLFLMVVSLITVIVLLILNAFIETAQEFRVVLCASVVALAH
                     VLCGFYMTRWAFTKSNKLFMSVVMGGMGIRMMLVGIILVIVYKLVAIDIKLFIVTFGV
                     YYLLFQIVEIYFVNRGLQMKKLNNNTI"
     gene            complement(9222..9527)
                     /locus_tag="PK937_07995"
     CDS             complement(9222..9527)
                     /locus_tag="PK937_07995"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNH32550.1"
                     /translation="MPTGKHTTQSLSETMHALGPWIGWGWQFALTLGVLTWGGHWLDG
                     YFETKVLFTLAGIFLGLAGGFIQLIRMVRNLPKPERHHHGEERHSDRVTKPTNEEKE"
     gene            complement(9533..9973)
                     /locus_tag="PK937_08000"
     CDS             complement(9533..9973)
                     /locus_tag="PK937_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNH32551.1"
                     /translation="MAFGEKNTNNTAGAGLNLISRGTVINGNISASTSIRIEGEIKGK
                     IVCHDAVTIGTTGMVEGDIDAKSVIVGGKVIGNVTSQDRLMMESRSGIQGNIKARRLV
                     VEEGAVFEGRCSMKDTKDKPLVINENANRDMFEEPEAARLAKAQ"
     gene            complement(9993..10943)
                     /locus_tag="PK937_08005"
     CDS             complement(9993..10943)
                     /locus_tag="PK937_08005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH32552.1"
                     /translation="MEEKKKSPHKYVTLLVMFDAVSDPRSFRLKVSTLRVMLGTLAVV
                     GLVLAGSLIYYFSFAYKVFYYDELERKYEKLADDNTRIKQIEREYRKVKQENEKIRIV
                     FGLLKNMPRDTTYEGRSTYENFTAPSEMGYYPGKDLNDPMHEKMAEDISSDVGRIGID
                     YLSYARVVPTLMPVNSRFIARGYQDNRYSDPSATRIHRGVDIVAEEGAPVKAASEGRV
                     IVSEWFTDYGNTIVLYHGFGFFSVYKHLQFRLKETDDAVKTGEVIGTVGRTGRLATGI
                     HLHFEIWRDGVPRDPATFIPQIRDALVITVKADSLAKKSS"
     gene            11197..12036
                     /gene="fdhD"
                     /locus_tag="PK937_08010"
     CDS             11197..12036
                     /gene="fdhD"
                     /locus_tag="PK937_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNH32553.1"
                     /translation="MESATRTYLIKKFSDGLDRDHADTLVTEEPLSIAVAHMERTVEI
                     AVTMRTPGHDADLALGFLAAEGLLHAGDGSPDYTIIQKIISPEENIITLHVNESAFAR
                     MAEADQLRRYSFVQSSCGICGSRSISDLRAKGFRRIEDAAVFMAEPILALPDRLRREQ
                     KIFEQTGGLHGAGLFDNEGNLLCAREDIGRHNAVDKIIGWTFMQRRLPLHGHLLLVSG
                     RVSYEIVQKALSVGIPCIAAVSAPSSLAVKSAHEFGMTLIGFLRSGHYNIYCGAQRIV
                     QHA"
     gene            12127..15000
                     /locus_tag="PK937_08015"
     CDS             12127..15000
                     /locus_tag="PK937_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32554.1"
                     /translation="MVTSTPMHPLKKLTLIFCILLMPSSDVSGQIKIHRSVNVEQGLV
                     QSQVTAITRDRWGYVWIGTLGGLSRWDGVRFQNFQTQDGLAGGQISALHEYHDGSMYI
                     GTDGGGLSVYRDGRFTTLNKDSGLCDNRVWNIIPSGDSALWICTYGGGVSFYQPQRRS
                     WKTIGLHEGLPDLRVYTGVHAGNDVWWFGTDSGVAILSPQTQQRITARDGLIHPIVRS
                     IRRLNDSAWALGTREGLSVYINGRCTNYTTRDGLADNDINAMLLLQNGALCIATANGI
                     SLWHHGVFENLTTANGLANDLVWNIYEDNDRTLYFGTNGGGVSMYRPGRLTTWRSGKE
                     LPDAPFTTVTQRRDGSMVFGSHAGLITMRGDKPDILDEKKGLAHNLVWSLFEDRRGRL
                     WIGTHNGIHTVTDGRVTATYHHSDKPMNRAYGMTEDADGNMILATRGGLAKIEDRRIV
                     PWATGTPLSGQIVWCARTLRDGRMAFGSDGAGLFIYDGSQFKNISQKNGLAGDFVVSI
                     YEAPDGLLCVGTDGGGLSLIRDGVIRNFDTKNGLRDNTIYAITGDTRGAIYAASNRGM
                     HILSFSGGDTLIRTMRRSDGLAGDECSLGGVCTDQNGRVWVATVNGMTEINPSSDSLY
                     TVPQTRIHRLRVYENSIPVVAHTAFRHDENYLKFDFIGIYPSAPEQVRYRYRLQGLDA
                     QWIPTTERTIQYTNLASGDYTFEVQSANDWGVWSTTESLVFTITPPFWKTWWFRLILV
                     LAVIGLLWIIYRVRVTRLLAMERMRVRIASDLHDDIGSTLTRIAVSSESIRSSDDLSA
                     IRHSAERIGQLSRDVIRTFSDIVWSIDARNDRMGDLIARLQDTAYQVLNTAEIACTWD
                     VRGIRADESLAVDVRQNVFLIVKEVLHNAAKHSEATSMHVRIERTDRGLMISLRDNGR
                     GIPDERIGKGNGLRNMRMRADRVRGRIKQINDNGSVTEIFIPL"
     gene            15030..15863
                     /locus_tag="PK937_08020"
     CDS             15030..15863
                     /locus_tag="PK937_08020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH32555.1"
                     /translation="MDYLDYQYDPNDPALMAVTDELPFWSAPFGTELLNAIRFKPGMT
                     VLDIGCGSGFPMLEIAERIGKKGTVWGIDPWPAAVARLREKTERLGITQVHIIEGQAE
                     DATIGEHTCDLIVSNNGINNVSDIPKTLSTCRRSAKHGAQFVMTYNLEGTMRAFYDVL
                     RAALRDHRREENLPLIDQHIRQKRPPLDRMEAWIEESGFEIGEIKLRQFHMDFTDGKA
                     FFEHHLIRHWFLPSWKNLVPQEILRPVFTAMENRMNDIVADDDVFRIEIPFALIDATA
                     I"
     gene            15976..>16680
                     /locus_tag="PK937_08025"
     CDS             15976..>16680
                     /locus_tag="PK937_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH32556.1"
                     /translation="MKLEAYFQKFRTGTIGHDLTVQTPYGTQHIVYADWVASGRLYRP
                     IEEQLLSRFGPYVANTHTETTTTGTTMTHAYHEAKAIIKGHVNAAPNDVLIFAGSGMT
                     GAVAKFQRILNLRIPEKFSGRISVPEAERPVIFVTHMEHHSNHTSWLETIADVELVGR
                     DEKGLVDLNDLSRLLERYKNRPMKIASVTACSNVTGIQTPYHDIAKIMHQNGGYCFVD
                     FACSAPYVPINMHPADS"
     assembly_gap    16681..16690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16691..17468
                     /locus_tag="PK937_08030"
     CDS             <16691..17468
                     /locus_tag="PK937_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH32557.1"
                     /translation="PADTDARLDAIYFSPHKFLGGPGTPGVLIFNAQLYGNKIPDQPG
                     GGTVTWTNPWGEHKYFQDIEIREDGGTPPFLQAIKAAMAIRLKETMDPSRMMAREEEM
                     LPRVFQVLRQAPGLHILADHVEHRLGIISFYIDGLHYNLGVKLLNDRFGIQVRGGCSC
                     AGTYGHYLLNVTREFSKRITDEIDHGDMSEKPGWIRLSLHPVMTDQEVDFILRAISDV
                     ATHHKTWSKDYVYDGSANEFRHKEFKENSAAITDWFRVEA"
     gene            17544..18170
                     /locus_tag="PK937_08035"
     CDS             17544..18170
                     /locus_tag="PK937_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH32558.1"
                     /translation="MIRIAMIEDDAEIRQNVTAFLKKDNRFTITASAGSVEEGLEAFK
                     THVPDILILDIGLPGMSGLSAIRLFREKFPDTDIVMFTVHDDPARIFEALCSGASGYL
                     IKTTPFADIARSLNELHNGGAPMSPQIARKVIQHFYPAPSQKPQSTLSDKEKEVVVGL
                     VDGLSYKMIADRMGVTIETIRSHIKNIYRKLHVHSKAEVITKSLRGEI"
     gene            complement(18274..18819)
                     /locus_tag="PK937_08040"
     CDS             complement(18274..18819)
                     /locus_tag="PK937_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HNH32559.1"
                     /translation="MKRLTVLIGFVCCPILSLFAQYQIDATESVHVPVLIKSLSYDSK
                     LKGGNLLILSRTDENSNRSKASSMFSGKTIKSNTLQAFDGTYSGKAALASILDNLGVH
                     LIYICDDLTAAQVSEISALCNAKSILSISGVTDHVATGLVSLSVSNEGGKTKTFLNKT
                     KMRAEGHAFSAQFLQLCKIVE"
     gene            19143..19595
                     /gene="rnhA"
                     /locus_tag="PK937_08045"
     CDS             19143..19595
                     /gene="rnhA"
                     /locus_tag="PK937_08045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNH32560.1"
                     /translation="MTTSQSPHITIYTDGACKGNPGRGAWAAVLLAEDGKSKEISGTE
                     DATTNNRMELAAVAEALKALKRPCRVTLHSDSAYIVNAFHQKWLDNWQRNGWRTADKS
                     PVKNRDLWETIINLCQTHTVRFEKVKGHAGIHYNERVDTLCNEALSVQ"
     gene            19592..20428
                     /locus_tag="PK937_08050"
     CDS             19592..20428
                     /locus_tag="PK937_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH32561.1"
                     /translation="MKRLIWRIEYFILLFFRYVLLVLPLPLIPYIGALIGDVFHFFKI
                     RHNVAYSNLVRTGLYDPSAIQRILRQTYRQFGKTFAELLVLDRYNDAHAKQFSFRLPE
                     DWDWHNLRSSVLISAHFGNWELMGKALIAHGVPLAVVVRRQTNPYVDAWLFRHRTRCG
                     MKVLYEDDIYGILRTLRSGHALALLSDQDFGHNKIPVNFFSQPCFAPAGPEMLARRTG
                     CRAWICLGKRETSDQFVFSIDTISAPETSFSSHYTALIESAIRSAPEQWFWFHRRWKN
                     NK"
     gene            20499..21782
                     /locus_tag="PK937_08055"
     CDS             20499..21782
                     /locus_tag="PK937_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32562.1"
                     /translation="MSLKRLPHSQDTGDSNTLNHHEILVVDDEENVLSVLSRLLSDEY
                     KVHTATNGKDGLEILEKNPNIALIISDQRMPVMSGTEFLNRSISVNPDCIRIILTGYT
                     DVKDLIESINAGRVFQYVTKPFETDDLRIIVKRSLDFFAQSRALERAHLEIKTAYENL
                     KNAQEQLVRSEKMSMLGKLMSSIAHEIRNPINNISNSTKLISLEWADVKNLFIEVNAL
                     TTGQKTAEQFKSAVKDRYNIEEVLQNFETAVGVIKHSCDLVTEIVEDLRGFSRLDDAE
                     FVQTDIHQQIERSLTLLKAKFKHHIEFVKDFGVVSHILGLPGPLSQVLINLINNAAQA
                     SPADGKVTIQTRQENTTVRIAITDNGAGIPKDIIGKIFDPGFTTKPESEGTGFGLSIS
                     AEIIKKHNGRIEVVSAPGMGSTFTVYLPVDPRNVS"
     gene            21786..22337
                     /locus_tag="PK937_08060"
     CDS             21786..22337
                     /locus_tag="PK937_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH32563.1"
                     /translation="MHRNWLISRLNAYHPSDPHESAMRDRMLNFVQTYPDCFERYLSI
                     GHITGSAWIVNPSRDKVLLTHHGKLNRWLQLGGHADGDPHVDRVALREAQEESGVEAT
                     TLLSDMPFDLDIHEIPPHKSDAAHLHYDVRFLIEIDDAIPLRISSESKDLAWIALDRI
                     ADYTTEESVLRMARKTAVMQNNL"
     gene            22383..>22988
                     /locus_tag="PK937_08065"
     CDS             22383..>22988
                     /locus_tag="PK937_08065"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNH32564.1"
                     /translation="MLSNQALDPHFRWRGHEVTRVEAFSDAVFAFAMTLLVVSLEVPK
                     NLSQLLETMRGFPAFATCFTILVMLWYWHYKFFRRFGLQDRFTIVWNAALLFVVLFFV
                     YPLKFVMTLVVNMMLGVAYEQPPIAAGQGDDMMYIYNAGYIAVCGIYALLYHHAYRLR
                     ARFELNEAEVFIARSERRAYIINIGIALFSTSLLLYGGSAMA"
BASE COUNT         5774 a         5846 c         5394 g         5964 t
ORIGIN      
        1 tctctaagac ataaatccct ctttgccggc tttgcgcctt cgcgaggata tatgaaacgc
       61 tatcgttccc ggaaaataac tccgtattta ccgggggcct tgtcccataa aacaaaaagc
      121 ccccgcaata catgcggggg cttttgcgta tcaaacacaa tcttgttcta ttgtttgttg
      181 tatttctctg caagacgcaa acgattgcgc gaacgaagca acgcctgacg cgaacgttcg
      241 ggatcgtagc tggaacgatc ttcaaggcgt ttgcgggcgc gttcgtaaga ctcctgagcg
      301 cgaatcaagt caatcgaact gactttttcg caggtctccg caagaaaagt gactttattg
      361 tgttccactt ccacaaaacc accgctcgtt gcataaattt cgtcataccc gtccgtgtga
      421 cgaattttga cttcaccgat aagtaccgat gaaatataag ccgtgtgatt tttgaggatc
      481 tggaaactac cactcgcacc cggggccgta aaactttcaa cttccccttt gaacacgaca
      541 tggcgcggtg agatgatttc caaatgatat ttattattgt ccatgatatt tccttcgttt
      601 accgattatc ggcgcatctt ttccgctttt tcttccgctt catctatggt acccaccata
      661 tagaaggctt gttccggcag atcatccatc tgaccttcgg cgatcgcttt gaaaccggca
      721 atcgtatctt ctttcttcac atagcggccg ggcgtaccgg taaactgctc tgcgacgtgg
      781 aaaggctgag ataagaaacg ttgaatttta cgggcacgcg ctacgacgat cttatcgtca
      841 tccgacaatt cgtccatacc gaggatcgcg atgatatcct gaagatcttt atatttctgg
      901 agaatttcct gcacgcgttt cgccgtatcg tagtgttcct gacctacgac gagcggatca
      961 agaatacgcg acgtggaatc caaaggatcc acagccggat agataccgag ctcggagatc
     1021 gcacgcgaaa gcacggtcgt ggcatccaag tgtgcaaacg tcgtcgccgg tgcgggatcg
     1081 gtcaaatcgt cggccggcac atatacggcc tgtacggatg tgatcgaacc ttttttcgtt
     1141 gatgtgatac gttcctgaag ttcacccatt tccgttgcaa ggttcggttg gtaacccaca
     1201 gcgctcggca tacggccgag gagcgcggac acttcagaac cggcctgcgt aaaacggaag
     1261 atattatcaa taaacagaag cacatctcgg ccttcgtcat cacgaaagaa ttcggccatc
     1321 gtgagggccg tcaacgcaac acgctgacgc gcgccgggcg gctcattcat ctgaccgaat
     1381 accagcgcgg ttttattgag tacaccggaa tctttcattt cgtgatacaa atcatttcct
     1441 tcgcgggtac gctcgcctac gcctgcaaat acggaatagc cgccgtgctg tttggcgata
     1501 ttgtgaatca attcctgaat caacacggtt ttgcctacac cggcgccgcc gaaaagaccg
     1561 gttttaccac cgcgcatgta cggttcgatc aagtcaataa ccttgatacc cgtttcaaac
     1621 atttcacttt tggtggtcag ttcttctaat ttcggagcag gacggtgaat gggcatacgt
     1681 ttgttggagg cttcgatttt accgaatccg tcaatcgtat cgccggtaat gttaagcaaa
     1741 cggccgagta ctttttcacc gaccggtacg gcgatcggag cgcccgtgtc cacggcatcc
     1801 attccgcgca caaggccttc cgtcgcatcc atagcgaccg cacgcacacg gttttcgcca
     1861 aggtgctgct gcacctcgac aaccagcgtc gaaccgtcgg gacgcgtaac atgagccgaa
     1921 ttgtaaatct ccggcaattg gccgttggca aactcgatgt ccaccacagg gccgatgatc
     1981 tgaacgattt tacctttgtt catgagtgtc gatccttaat tctatatgtg ttaaattatt
     2041 tcgtattcca aaaattcatt tgtattccaa aattccaata cgtgtacgtt tgtttttcgt
     2101 ataacccgcg actacgtgtc caatctatac gcggtgtaat atcccattgc gatatatcta
     2161 aaactttaag aggtataatc accgatgcac cgtacgcgcc gccaatgagt ttttctttat
     2221 atcgcggttt atttttttgt ttttcaaaat ttttagtctg tgtacgcaaa gataatatat
     2281 caaatatagt ccattccgtt ccgacacgca gttcatcatc aagcacatta gcatctttgt
     2341 aaactaactg aatcatacct ttgaatacgg tgaagcgcca cgcatcagat tggtatgaaa
     2401 gtccgtacgc aaaagcccag tagtcgaatc gctgcacctt gatcggatcg ccaccgaaaa
     2461 atacaaaaga agtatcgtta ctcattggat ccacgatggt ctcatggtaa tcgctcttat
     2521 acacaggttt tttccctgcc cgattattaa tactgctaaa tcccgcacta atttcgttgc
     2581 gataaccgcc agtgttgaac catacgagtg gaagtctcgc cataataccg atatcattaa
     2641 attgtgcttt agcggtaaac aaatcttttt tggaataaat ccatttatgc gaaataccca
     2701 cggcaagcca accggaccat gcatacgcat aagaagcgga aaatgcgttt tcatatggac
     2761 gataatattc gcctgtacca tccggatttt cttcgttggt acgctctatt cgtggatagt
     2821 tgaagcgata attatttacc gctagtgaac cgaaacgcgt atgccccata cctgccgtcc
     2881 agtaattgat cgaggtattg gcgacatagt cattcgtgtt atagctcata cccaggccgg
     2941 atgcaaatgc aatgcccgcc ggattgtaaa aagctgacca cgcattgggg atagccacca
     3001 ttggaccgat cgccgcagaa cgtgcgtccg gacggattcc gagaaatgta aaaccgacag
     3061 cattctcttc tccggccgtc agacgcgtaa ccatcgtagc gactaaaaaa acgtaccgta
     3121 ttgtcaaaga acttccccta aacatacccc atccctctac gtaaaaattt acttccgcaa
     3181 ggcctctgca ccggaaacaa cttccaatat ttctttggtg atcgcggctt gtcgtgcacg
     3241 gttataatac aaggtcaacg tacgaatcaa atcgcccgca ttctccgtcg ccgactccat
     3301 cgccgtcata cgcgcaccca tttcggcggc aaatgattcc agcagaatac gccacatctg
     3361 cacgttcaga tgacgcggga tcagcgtatt gatgatttcg tattgcgaag gttcgtagat
     3421 ataatccgtt tcgatgttgt acttcacatt ggacgattcg actttttcag gttcaaccgg
     3481 caaaagtctt tccatcacga gattttgctg aatcgccgat ttgaattcgt tatacacgac
     3541 atgcacttca tccacacgtc cggccgaata ttcctgcacg caataggtga tcactttcga
     3601 agcatcgtca aaattgagat gattgaaaaa attggcatga cgaaatacga caggaaaatg
     3661 acgtttggta aaaaattcat cgcctttacg cccgaccgta accaatttta cattattgtt
     3721 atccaatttg gacttgaggt cgcccttaag caggttattg acataacgaa taatgttggt
     3781 attgaacgcg ccgcacagcg aacgatcggc cgtcacgacg acgatcatga tgtttttcac
     3841 atcgcgcggt ttgatcagcg gattttgctg cacttcttca ttgcgtgcca gatgtttgat
     3901 cagcgcaccg agtttgtaag catacggacg cgcggaaata atattttcct gcgcacggcg
     3961 gagtttagcc gccgccacca ttttcatggc cttggtgatt ttttgcgtac ttttgacgga
     4021 tacgatgcgt cgtttgattt cgcgtaatgt cgccatagtg tgtgtttact ttttggattc
     4081 gatgaccgcc gatgtgtcgg gcttctcatt gagagtccgc agcgtcgctt cgttcatccg
     4141 ttccagaaat tcgttttctt tgtttttgtt tttgctgatg atttctgacg tggtaaactt
     4201 catatccgcg ccggtatagc cgtacggctt tgcaaatgct tccagatccg ccgccgtggc
     4261 ctcttcaaaa ggtttggcat atttggcttt gaaagcggaa tcactctgcg cctgaatcca
     4321 gatgtccaca aacgtttcca tcggaacttc ctgcgctttg ggcgcacatc cgtacaatgc
     4381 cgcgccgata caaatcagag taataatgcg tgcgatacgt ttcatttggt catcagttgt
     4441 ttgaaaacag tggaatcgtt ctgacttgct ttttccaagt cgctctgcag caatttgccg
     4501 atacgcacag ccagatcatt ttttttggcc gcatcgttgt caaaaagttt tacggtatga
     4561 cggtaatccg acggcgtaaa accaagtttt ttgacgatcg gttccagatc ggcatcgggc
     4621 aattcctcga acttcacatc atccacgagg aacgatttgc tgcgcatgac ttcgtaagtg
     4681 atcgtggcca tcggttccgc cggtatttct ttgggaccgc aaccggccaa catcacccaa
     4741 ccgaaaagaa gcagtacgtt tcgcataaag atttccgttt agtcttcttt tgtaactttg
     4801 aacgcggctt tgaagtcgcc gatgatcgta ttcagtttgt ttttgatgtc gtccgtcagc
     4861 tctttctttt ccatgatcgc cgacaaaacg tctttgtgac gcagttccat cgtttctgtc
     4921 agttcttttt caaaacgacg gcaatcaccg accggcacat catcaaggaa tccgttggtc
     4981 gcggcataga tgatggtgat ctgcgcatca aacgacatcg gctgatattg accttgtttg
     5041 aggatttcca ccaaacgcgc accgcggcga agctgctgct gtgtcgcttt gtcaagatcg
     5101 gaaccaaatt tagcaaatgc ctcgagctcg cgatactggg ccaattccaa acgcagacgg
     5161 ccggctactt gtttcatggc tttgatctgt gcgctgccgc ctacgcggga cacggaaata
     5221 ccgacgttga tcgcaggacg tacgccggaa taaaacaaat tggattcgag aaatatctga
     5281 ccgtcggtga tcgaaatcac gttggtcggg atgtacgccg acacgtcgct cgcctgcgtt
     5341 tcgatgatcg ggagcgccgt cagcgaaccg ccgccgagat cctgattaag tttcgcggcg
     5401 cgctcaagca agcggctgtg cagataaaat acgtcgcccg gatacgcttc gcggcccggc
     5461 ggacggcgga gcagcagcga catctgacga tacgctacgg cgtgtttgga aagatcgtca
     5521 taaatcacca gcgcgtgctt accgttatca cggtagtgtt cgccgatcgc gcaaccggta
     5581 taaggcgcaa gaaactgcaa cggtgcaggt tctgaagcgg cggccgaaac gaccgtcgtg
     5641 tacgccatcg ctccgttatc ttccaaaatc ttcaccactt tggcgatcgt ggattctttt
     5701 tgaccgatac cgacgtaaat acaaaataca tctttgcctt tttgattgat gatcgtgtcg
     5761 atcgcaacgg cggttttacc cgtctgacga tcgccgatga tcagttcgcg ctgaccgcga
     5821 ccgatcggga tcatcgcatc gatggctttg ataccggtag caagcggctc tttaacggat
     5881 tgacgttcaa taacgccgag cgctttgcgt tcgatcagag ttttttgttt ggcattgatc
     5941 gcacctttgc cgtcgatagc ttggccgagc gcgttgacga cacgtccgag catttccggt
     6001 ccgacggcca cttcggcaag gcggcccgta cgtttgaccg tatcgccttc tttgatgccg
     6061 gaagagtcac cgaaaagtac ggcacccaca ttatcctctt cgagattgag aagcatgccg
     6121 acgacgccgt tgggaaattc caccagctcg ctcgccatgg ccttggtcaa cccgtaaata
     6181 cgggcaatac cgtcaccgac ttgcagcacg gtacccgtct cataggtatc catctcgcgt
     6241 tcaaaagaag caagttgctt cttgagaatg ccggatactt catctggtct tacagaaact
     6301 gccatgattt aggttccttg tgttatatta tatttatttg tctctattcg ttcgaatgtt
     6361 ttacatcatc aatggtgata cacggccgta aacgccgatt tgaggcgctc cagctgatgc
     6421 ttgaccgtac cgtcaatcat cgtatctccg atgcgaacca taaatccgcc gatcaatgac
     6481 gcatcttttt tgaaaaccag ttgcggcgtt ttgccggtca gggccttgat tttttcggtg
     6541 acgcctttga cctgatcggg ggtcatatcc acgacggaga taacctccgc atggatgaaa
     6601 ccgagttttt cattacgcag cgtttcaaac gcttcgatca tgtcgcctaa aatgttttca
     6661 cggtcgtgat cgcagatcaa atgcataaag cgatacactt cagtgtgaat ttttttatca
     6721 aacagcgctt tcatcgcgtc ttttttctgc gcattgggga tcaccggatt gtgcaaaaaa
     6781 gcacggaatt ccggcgactg ttctatcatc gtggcgatgc tctgcatatc ggcatagacg
     6841 gcatccagcg cgttctgctc gaccgagatg tcaaacaggg ctttggcgta gcgtcgtgcg
     6901 gttgcggaat ttttcatatt actttggtat taatacttta acgttgtacc ggttaattaa
     6961 ttacgcccca ccggcatgtc tttgataaaa tcatcgatca gttttttatg tttatcggca
     7021 tccaggttgg tcgccaagat tttgccggcg gccgtgaccg ccatgtcacc gacttctttg
     7081 cgcaacgcac tgatcgcaga attcatttcg ttttcgatct gcgtacgggc ttccgctacc
     7141 aaacgggccg cttcggcttt ggcctgatcc atgatttcgg ttttcagttt ttcggcattt
     7201 tctttggcgc ggcgcgcgac ttcctgagcc tcacgttcgg ccttcgccat gatttcctga
     7261 tttttttcgg tcagagcggc cgcttcacgg cgggcttttt ccgcttcttc aagagaattt
     7321 tttatttttt cttcgcgttc ttcgagcatc ttgatgatcg ggccccatgc ggtcaggcgt
     7381 aaaagcacga gcaaaatgac gaaggtgact atggtccaaa agatcaaccc cggatcgatg
     7441 tccagcaatc cgcctttatg ttcgccgccg ccggagatca tgtgcagaag cagtaatgtc
     7501 ttcatagcgt acaaaacctt ttcagttaca actcagttta caacaacatc attaaacaaa
     7561 cggcgggagg gatttaactc ccgccgtatt ccaacgtaca atcaatacga ttacttcaat
     7621 gccaacagga tgcagataac ttctgcaaaa agcgccacac cttcgataag ggcggctgca
     7681 atgatcatgg tcgtacggat atcaccggcg gattccggtt gacgcgcact gccttcgagg
     7741 gcggaagcgg ccaatttacc gatacccaaa cccgcaccaa tcgtagccag acctgcgccg
     7801 ataccggcgg ccaagaagcc aaatcctaaa tcgcccattg tagtttcctc cgttaaatga
     7861 tgttgtaaat gagttcgttt atgtatttgt taattgcatt cgttatctat tcagtgatgc
     7921 cccgcatgca ccggatgctc cgcgtcatga tgctcttcat ggtgttcgtg gtgcataccc
     7981 atgctgataa aaagcgacga taacagcgta aaaatgtacg cctgcagaaa ggcgacgaaa
     8041 agttccaata cgtaaataaa caacgcgaac aggatagaaa tcggcgcgac aaaatatgtt
     8101 tttaatacga agatcaaacc aagcagggag agaatcacga cgtggcctgc cgtcatattg
     8161 gcaaaaagac gaacgctgag cgcgaaaggt ttggtaaaaa gccccactac ttcgacggga
     8221 atcatgatcg gccacaaaag ccaatgcacg ccggccgtaa gatgtttcag atacccgccg
     8281 ataccttgcg acttgatagc ggtatattgc gtgacaaaaa acgtcacgac ggccaaggtc
     8341 gctgtgacgg cgatattacc ggttgctgtc gaaccatacg ggataagccc cagaagattc
     8401 ataaacagaa taaagaaaaa caacgtgagg aaataatgga tatagcggtc ggttttagca
     8461 ccgaaattag ggcgcacaat ctcttcgcgc acgaagctga tcaacgcttc catggcattg
     8521 tagaggccgc gaggaatcag tttgggacga gccaagatgg aaaaaatcac caatagcaaa
     8581 agcgctacca accatatcat cacgacatgt ttggtcggac tgaggtctat cgtcatagtg
     8641 ccgatatgaa tgggatcaaa atgcggcagt ttcacataac ccaatatggg gagttcgatt
     8701 tcattggaat tggtaatgtg gtggatgata gtcccgccga tatccacttt ttctgctttg
     8761 tcggcggctt tgggcgcttc ctgcgaagcg gcttgccaga aggttaacat aggcatgagc
     8821 atgcgtacga atatcctata tagtattatt atttaatttt ttcatctgca gcccgcggtt
     8881 tacaaaataa atctcgacaa tctgaaacag caggtaatac acaccaaatg taacgatgaa
     8941 caacttgata tcgatcgcca ccaatttata cacgatcacg aggataatgc cgaccaacat
     9001 catacgaatg cccatgccgc ccatcacgac gctcataaaa agtttgtttg acttagtaaa
     9061 agcccaacgc gtcatataaa agccgcaaag cacgtgcgcc aatgctacaa cgctcgcaca
     9121 aagcacgact cgaaattcct gtgccgtttc aatgaaggca ttcaatatca aaagaacaat
     9181 gacggtgatc agcgatacga ccatcagaaa caatttttga ctcattcctt ctcttcattc
     9241 gtcggcttgg ttacgcggtc cgaatgacgc tcctctccgt ggtgatgtcg ttccggcttc
     9301 ggaagattcc gaaccatccg gatcaactgt ataaaaccgc cggccaatcc caaaaaaata
     9361 ccggccagcg taaaaagaac tttcgtttca aaatatccgt ctaaccagtg cccgccccaa
     9421 gtcaaaacgc cgagcgtcag ggcaaattgc caaccccatc cgatccacgg cccaagcgca
     9481 tgcatcgttt ccgataagct ttgggtcgta tgtttacccg tgggcataaa acctactgcg
     9541 ctttagctaa acgcgcagct tcaggttcct caaacatatc gcgattcgca ttctcgttga
     9601 tcacgagcgg cttatctttc gtatctttca tcgaacaacg accttcgaac accgcgcctt
     9661 cttcgaccac caaccggcgg gctttgatat taccttggat accggaacgc gactccatca
     9721 tcaaacgatc ctgcgaagtc acattgccga ttactttgcc gccgacgatc acgcttttgg
     9781 catcaatatc gccttcgacc atacccgtcg taccgatcgt gacggcatca tggcaaacga
     9841 ttttgccttt gatttcgcct tcgatacgaa tgctcgtcga ggcgctgata ttgccgttga
     9901 tcacggtacc gcggctgatc aggttgagac ctgcaccggc ggtattgttc gtattttttt
     9961 caccaaacgc catgggcgtt ctttcgttta gtttaactgc tttttttggc gagtgagtcg
    10021 gctttcaccg taatcaccag cgcatcgcgg atctgcggta taaaagtcgc cggatcccgg
    10081 ggcacgccgt cgcgccatat ttcaaaatgt aaatgaatac ctgttgccaa acgcccggtc
    10141 ctaccgaccg tgccgatcac ttcacctgtc ttgaccgcat catccgtttc tttcaggcga
    10201 aattgcaaat gtttgtacac tgaaaaaaaa ccgaatccgt gatacaggac gatcgtgttg
    10261 ccgtagtcgg taaaccattc cgaaacgatc acccgacctt cggaagcggc cttgaccggc
    10321 gcgccttcct ccgccacgat gtctaccccg cgatgtatgc gtgtagccga cggatcgctg
    10381 taacggttgt cctgataacc gcgcgcaata aagcggctgt taaccggcat cagcgtaggc
    10441 acgacacgtg cgtaggataa ataatctata ccgatacggc cgacatcgct gctgatatcc
    10501 tccgccattt tttcatgcat cggatcgttg aggtctttgc cgggataata tcccatttca
    10561 ctcggcgccg taaaattttc atacgtcgag cggccttcgt acgtcgtgtc ccgcggcatg
    10621 tttttaagca aaccaaacac gatgcggatt ttttcattct cctgcttgac cttacggtac
    10681 tcgcgttcga tctgcttgat gcgggtatta tcgtcggcga gcttttcgta tttacgctcc
    10741 agctcgtcgt aataaaatac cttataggca aacgaaaaat agtatatcaa agaaccggcc
    10801 agtacaagac ctactacagc caatgtaccg agcatcacgc gtagcgttga aactttaagg
    10861 cgaaaactcc ggggatcgga aacggcatca aacatcacca gaagtgtgac atatttgtgc
    10921 ggcgatttct ttttttcttc cacgttcaac ccgtttttga tgaccgatcg gatcgcgttt
    10981 atatcgcttt aaaacgatgc taaatctaaa tttttacaga acatacgcct ttttttccaa
    11041 aaagcaacgc aatatatcta aaagaacatg atttgtcaat ttttttctta attataagcc
    11101 gatccggaaa ttaacacctt tataccataa aaataccata aaattagtga aattgaagcc
    11161 ctttttgggt agtttgtgcg aaacttcgag gtttatgtgg aatcggcaac acgcacctat
    11221 ctcataaaaa aattcagcga tggtttggac cgcgatcatg ccgacacgct tgtcactgaa
    11281 gaaccgctgt ccatagcggt cgctcacatg gaacgcaccg tcgaaatcgc cgttacgatg
    11341 cgtacacccg gtcatgacgc cgacttggcg ctggggtttt tggcggccga aggcttactt
    11401 cacgccggag acggctctcc cgactatacc atcatacaaa aaatcatatc accggaagaa
    11461 aatattatca cactgcatgt gaatgaatcc gccttcgccc gcatggccga ggccgaccaa
    11521 ttgcgtcgct attcgtttgt ccaatccagc tgcggtatct gcgggagccg ctcaatcagc
    11581 gatttgcgcg ctaagggatt tcgccgcatc gaggacgcgg cggtttttat ggccgagcca
    11641 attctcgctt tacccgatcg cctgcgccgg gaacaaaaaa tattcgagca aacaggcggc
    11701 ctgcacggcg ccggattatt tgataatgaa ggaaacttac tctgcgcgcg cgaggatatc
    11761 ggacggcata acgctgtgga caaaatcatc ggctggactt tcatgcagcg acgtttgccg
    11821 ctccatggtc atctgctgtt ggtcagcgga cgcgtcagtt acgaaatcgt gcagaaagcg
    11881 ttgagcgtcg gcataccgtg tattgcggcg gtttccgccc cgtccagtct ggccgtcaaa
    11941 tccgcacacg agttcggtat gacattgatc ggatttcttc gctccggcca ctacaatatc
    12001 tactgcggtg cgcagcggat cgtacagcac gcatagttgt ttcgtcaggt cacgtatccg
    12061 tatccgcgca tctcgaaaat aatcggttct tattttcata aatcacgtct gatggcgcag
    12121 tttaatatgg taacatcaac cccgatgcac ccgctaaaaa aactaactct gattttttgt
    12181 atactgctga tgccgtcatc cgacgtcagc ggtcaaatca aaatccaccg ctccgtcaat
    12241 gtcgaacaag ggcttgtcca gtcgcaagtc acggctatca cccgcgaccg ttggggttat
    12301 gtatggatcg gcacgttggg cgggctgagc cggtgggacg gtgttcgttt tcaaaatttt
    12361 caaacccaag acggacttgc cggcggacaa atatcagcgt tgcacgagta ccatgacgga
    12421 tctatgtata tcgggaccga cggcggcggc ctctctgtgt atcgcgacgg acgttttacg
    12481 acacttaata aagattccgg tttgtgtgat aatcgcgtgt ggaatatcat tccgtccggt
    12541 gattcggcgt tgtggatttg tacgtatggc ggcggcgtca gtttttatca accgcaacgg
    12601 cgaagctgga aaaccatcgg ccttcacgaa gggttacccg atctgcgcgt gtacaccggc
    12661 gttcacgccg gtaacgatgt ctggtggttt ggcaccgaca gcggtgtagc gatcctgtcc
    12721 ccacaaacgc aacagcgcat cacggcacgc gacggactga tccaccccat tgtacgcagc
    12781 attcgccgtc tcaatgatag cgcatgggct ttgggtacac gcgagggcct gagtgtgtat
    12841 atcaacgggc gttgtactaa ctacaccaca cgcgacggtc ttgccgataa tgacatcaat
    12901 gcgatgctgc tcctgcaaaa cggcgcattg tgcatcgcta cagccaacgg catcagtcta
    12961 tggcaccacg gcgtttttga gaatctgacg acggccaacg gattggccaa tgatttggtg
    13021 tggaatatct atgaagataa tgaccgtacg ctttatttcg ggaccaatgg cggcggcgtc
    13081 agcatgtatc ggcccgggcg tttgacgacg tggcgcagcg gtaaagaatt gcccgatgcg
    13141 cctttcacca cggtcactca gagacgcgat gggagtatgg tgtttggttc acacgccggc
    13201 ctcatcacca tgcgtggcga taaaccggac atcctcgacg aaaaaaaagg attggcgcac
    13261 aacctggtct ggagtctttt tgaagatcgt cgcggacggt tgtggatcgg cacacacaac
    13321 ggcattcaca cggtaaccga cggacgcgtg accgccactt atcatcactc cgataaaccg
    13381 atgaatcggg cttacggaat gaccgaagat gccgacggga acatgatttt ggcaacacgc
    13441 ggagggcttg ctaaaataga agaccgtcgg atcgtgccat gggccaccgg gacgccgctc
    13501 tccgggcaga tcgtatggtg cgcacgtacg ttgcgtgacg gccgcatggc ctttggcagc
    13561 gacggcgccg ggctttttat atatgacgga tcgcaattca aaaatatttc tcagaaaaac
    13621 ggactggccg gcgattttgt tgtttcgata tacgaagcgc ccgacggcct tctgtgcgtt
    13681 ggtaccgatg gtggcggctt gtctttgata cgcgacgggg ttattcgtaa ttttgatacc
    13741 aaaaacggat tgcgtgacaa tacgatctat gcgatcaccg gtgataccag aggcgccatt
    13801 tatgcggcca gcaatcgcgg catgcacatt ctttcgtttt ccggcggaga cacgctgata
    13861 cgtacgatgc ggcgcagcga cggcctcgcc ggcgatgaat gcagcctcgg aggtgtgtgt
    13921 acggatcaga acggccgggt atgggttgca accgtcaacg ggatgacaga gatcaatccg
    13981 agttccgata gcctctatac cgttccgcag acgcgtatcc atcgtttgcg cgtgtatgaa
    14041 aatagcattc cggtggtggc gcacacggct ttccgccatg atgaaaatta tttgaaattt
    14101 gattttatcg gaatctatcc ttcagcgccg gaacaagtgc gctaccgcta ccggctacaa
    14161 ggtcttgatg cgcagtggat tcctaccacg gaacgcacaa tccaatatac caatctcgct
    14221 tcgggcgatt atacgtttga ggtgcagtcc gccaacgact ggggcgtatg gagcacgacg
    14281 gaatcgctgg tttttacgat cacgcctccg ttttggaaaa cctggtggtt ccggttaata
    14341 ctcgtcctgg ccgtgatcgg attattgtgg attatttacc gtgttcgggt aacgcgattg
    14401 ctcgccatgg aacgtatgcg cgtgcgcatt gccagcgatc tgcatgatga tataggttca
    14461 acgctcacgc gtattgccgt cagttcggaa tccattcgca gttccgatga tctctcagcc
    14521 atccgccatt ccgccgaaag gatcggccaa ctcagccgtg atgtgatacg caccttcagt
    14581 gatattgtct ggtccattga cgcacgcaat gatcggatgg gcgacttgat cgcacgcctg
    14641 caggacacgg cgtatcaggt gctcaacacg gccgagatcg cctgcacgtg ggatgtacgc
    14701 ggtatccgcg cggacgaatc actggctgta gatgtgcggc aaaatgtttt tctgatcgtc
    14761 aaagaagtgc tgcataatgc ggccaaacat tcggaagcca cctccatgca cgtccgcatt
    14821 gagcgtacag atcgcggtct tatgatttct ctgcgggaca atggccgtgg cattcccgac
    14881 gagcggatcg gaaaaggcaa cggccttcgc aatatgcgca tgcgcgccga tcgtgtgcgc
    14941 ggccgcataa aacaaatcaa cgataacgga tcggtgaccg aaatttttat acctctgtaa
    15001 aataatttca ctaataaaag aaaaaccgta tggattattt agattatcaa tacgatccca
    15061 atgatcccgc actgatggcg gtcaccgatg aattgccgtt ttggtcggcg ccgtttggaa
    15121 cggaactgct caacgcgata cgctttaaac ccggcatgac cgtgctggat atcggttgcg
    15181 gcagcggatt cccgatgctt gaaatcgccg aacgtatcgg aaaaaaagga acggtatggg
    15241 gcattgatcc gtggccggcg gcggtcgcac gcctccgcga aaaaaccgaa cgtttgggca
    15301 tcacgcaggt gcatatcatc gaaggtcagg ccgaagatgc aaccatcgga gaacatacgt
    15361 gtgatctgat cgtatccaat aacggtatta ataatgtcag cgatattcct aaaaccttaa
    15421 gcacgtgccg ccgttccgcc aaacacggtg cgcaatttgt catgacttat aatctcgaag
    15481 gtacgatgcg cgctttttac gatgtgttgc gtgcagcgtt gcgcgatcat cgccgcgaag
    15541 aaaaccttcc ccttattgat cagcatatcc ggcaaaaacg acctccgctg gatcgcatgg
    15601 aagcatggat cgaagaatcg ggatttgaaa tcggagaaat caaactgcgg cagtttcaca
    15661 tggattttac cgacgggaaa gccttcttcg aacatcattt gatccgccat tggtttctcc
    15721 cttcatggaa aaatcttgtg cctcaggaaa tcctgcgccc tgtttttacg gcgatggaaa
    15781 accgcatgaa cgatatcgtc gcggatgatg atgtgtttcg catcgaaata ccttttgcct
    15841 tgattgatgc gacggccata taaaaaaccg cgattttatt tacagaaaat ccgatgattt
    15901 tttgcatgca gttggttgtt tttttaatta tcctctcggg tttaaaaatc attaaccttt
    15961 atacactctg cattcatgaa acttgaagcg tacttccaaa aatttcgcac cggcaccatc
    16021 ggtcacgacc tgacggtcca aactccgtac ggaacacaac acatcgttta cgccgactgg
    16081 gtggcaagcg gacggctgta tcgccctatc gaggaacaac tcctttcgcg gttcggccca
    16141 tacgtcgcca atacgcacac ggaaaccacc accaccggaa cgacaatgac gcatgcgtac
    16201 cacgaagcta aagcgatcat caaagggcat gtaaacgccg ctcccaatga cgttttgatt
    16261 tttgcgggtt cgggtatgac cggtgcggtc gccaagtttc aacgcatact caatcttcgc
    16321 attcctgaaa aattcagcgg acgcatttcc gtacccgaag cggaacgccc cgtgattttt
    16381 gtaacccaca tggagcatca ctccaatcat acctcatggc tggaaaccat cgcggatgta
    16441 gaattagttg gtcgtgatga aaaaggtttg gtggatctga atgatctttc acggttactg
    16501 gaacgctaca aaaaccggcc gatgaaaatc gcttccgtca ccgcatgttc caacgtcacg
    16561 gggattcaaa cgccctatca cgacatagct aaaatcatgc atcagaacgg cgggtattgt
    16621 tttgtggatt ttgcatgctc cgcgccgtat gtgccgatca atatgcatcc tgcagatagt
    16681 nnnnnnnnnn ccccgccgat accgatgcgc gcctggatgc tatttatttc tctccgcata
    16741 agtttctcgg cggacccggc acgcccggtg ttttgatttt caacgctcaa ctttacggta
    16801 ataaaattcc cgatcaaccc ggcggcggta cggtgacatg gaccaatccc tggggcgaac
    16861 ataaatattt tcaggatatc gaaattcgcg aagacggcgg tacgccgcct tttttgcagg
    16921 cgatcaaagc cgcgatggcg attcgcctca aagaaacaat ggatccgtcg cgcatgatgg
    16981 cgagagaaga agaaatgttg ccgcgtgtat tccaagtttt gcgtcaggcc ccggggttgc
    17041 acatactggc cgatcatgtc gaacaccgcc tcggcatcat ttcattttac atagacggtc
    17101 tgcactacaa tctcggcgtc aaactgctca acgatcgctt tggcattcaa gttcgcggcg
    17161 gctgttcctg tgccggcacg tacggacatt atttactcaa tgtgacgcgc gaattttcca
    17221 aacgtatcac ggacgaaatt gatcacggcg acatgtccga aaaaccgggc tggataaggc
    17281 tttcccttca tccggtgatg accgatcagg aggtggattt catccttcgc gcgattagcg
    17341 atgtggcgac ccatcacaaa acatggtcga aagactacgt gtacgacgga tcggccaacg
    17401 aatttcgtca caaagagttc aaagaaaaca gcgcggccat aaccgactgg tttcgtgtcg
    17461 aagcttagcc tcccatcgtt atgggattgg ttttctgcga tgacgtcctt agtttgcgat
    17521 gaatgtttta caaaggatcg tttatgatac gcattgcgat gattgaggac gacgcagaaa
    17581 ttcgccagaa tgtgacggca tttttgaaaa aagacaaccg ttttacgata acggcttccg
    17641 ccggatctgt cgaagaaggt ttggaagcgt tcaaaacgca cgtgcccgat attctgattc
    17701 tcgacatagg cttacccggt atgtcgggat tgagcgcgat acgtctcttt cgggaaaaat
    17761 ttcccgatac ggacatcgtg atgttcacag tacatgatga tccggcgcga atttttgaag
    17821 ccttatgcag cggtgcatcc ggttatctta tcaaaactac tccgtttgcg gatattgctc
    17881 gatcactgaa cgaattgcat aacggcggtg cgccgatgtc gccgcagata gcccgcaaag
    17941 tgattcagca tttttatccc gcgccttcgc aaaaacctca gtcaacgctg tccgataaag
    18001 agaaagaagt cgtggtggga ttggtggatg gtttgagtta taaaatgatc gccgatcgga
    18061 tgggtgtgac gattgagacg atccgctcgc acatcaaaaa tatttaccgc aaattgcatg
    18121 tccactccaa agccgaagtg atcaccaagt ctttacgcgg cgaaatctga cctttgaatc
    18181 tgtaaaaacc gtttcaaaca cccactgatg cggcatcaaa aatcattata aatttttgat
    18241 atattttttt gttggcaata taatgccgca atcttattcg acgattttac acagctgaag
    18301 aaactgggcg ctaaaagcgt gtccttctgc gcgcatcttg gttttattca gaaatgtttt
    18361 tgttttcccg ccttcattcg atacggataa actgaccaac cctgtggcca catgatccgt
    18421 caccccggag atactcagga tacttttagc attacacaaa gccgaaattt ccgatacctg
    18481 ggccgctgtc agatcatcac atatgtatat cagatgtacg ccgagattat caagtatgga
    18541 ggccaatgct gctttaccgg aatacgtccc gtcaaacgcc tgcaatgtgt tggacttgat
    18601 cgtttttccg gaaaacatcg aagacgcttt actgcgatta ctattttcat cggtacgcga
    18661 caatatcaga agatttccgc ctttcaactt actatcatac gataaacttt tgatcagcac
    18721 gggaacatgc acggattctg tggcatcaat ttgatattga gcaaacaaac tcaaaatagg
    18781 acaacacacg aaaccgataa gtacggtcaa tcgtttcaca cgatcctcca aaaaaggttt
    18841 ttttttattt gataagtgat aagacggaac cgccccaatg taaagaaaaa tgaagtgtaa
    18901 tcgcaagaaa tattactgcc gaaactattc aacacaactc gtatttttat atctatccgt
    18961 tctgcatccg ccctgcgatg ttcttacaaa ataccgatct tcaacggcct gcttttgcgt
    19021 gtaagcgata accatttaag aaaacgggag catcgggcgt ttcaaggtca agtccaatgc
    19081 agatatgatc ttattttaaa aaaaaatctt ttggttgatg ataattttta taaaatgtag
    19141 ctatgacaac atcacaaagc ccacatatca cgatatacac cgacggcgcc tgcaagggta
    19201 accccgggcg cggcgcatgg gcggctgttt tgctcgccga ggatggaaaa agtaaagaaa
    19261 tttccggaac ggaagacgcc acgaccaata accggatgga attggcggcc gtcgccgaag
    19321 cgcttaaagc gctcaaacgt ccttgccggg tcacgttgca ttccgacagc gcttacatcg
    19381 tcaacgcctt ccatcaaaaa tggctggata actggcaacg caacggctgg cgcaccgccg
    19441 acaaaagccc tgttaaaaac cgtgaccttt gggaaactat catcaatcta tgccaaacgc
    19501 ataccgtacg ctttgaaaaa gtcaaaggcc atgccggcat ccactataat gaacgcgtgg
    19561 acacgttgtg caacgaagcc ttgtcggttc aatgaaacgg ttgatctggc ggatcgaata
    19621 tttcatcctg cttttctttc gctatgttct tttagttctt ccactccctc tcattccata
    19681 catcggcgcc ctcatcggtg acgtatttca tttttttaaa atccgtcata acgtagcgta
    19741 ttctaatctg gtacgcacgg gtttgtatga tccgtctgcg atacaacgca ttctcaggca
    19801 aacataccgc caattcggca aaacgtttgc agagctctta gtattggacc gttacaatga
    19861 cgctcatgct aaacaatttt cattccgctt accggaagat tgggattggc acaacttgcg
    19921 cagctcggtt ttgatctccg cccattttgg gaattgggaa ttgatgggaa aagcgttgat
    19981 cgcgcatggt gttccgctgg cggtggtagt gcgccgtcaa acgaacccgt acgtggatgc
    20041 atggctattt cgacatcgca cacgttgtgg catgaaagtt ttgtacgaag atgatattta
    20101 cggcatactc agaacacttc gatccggtca cgctttggct ttgctttcgg atcaggattt
    20161 cggtcacaat aaaatacctg tgaatttttt ttctcaaccg tgttttgcac cggccggccc
    20221 tgaaatgctc gcccgacgca cgggctgccg ggcctggatt tgtctaggaa agcgagaaac
    20281 ttcggatcaa tttgtatttt caattgatac catttctgcg ccggaaacat cattctcatc
    20341 acactatacc gcattgatcg aatcggcgat ccgttccgct cccgaacaat ggttttggtt
    20401 tcaccgtcgc tggaaaaaca ataaatgatt attcttgatt taaactcact cagaacgtat
    20461 ttttttcaaa tttcaagcca ccatctcaat acaatcctat gtcgctcaaa cgtcttccgc
    20521 attcgcagga taccggagac agcaatacgc tgaatcatca cgaaatcctg gtcgtggatg
    20581 atgaagaaaa tgtattatct gtgttatcgc gccttttgag tgatgaatat aaagttcaca
    20641 cggccaccaa cggaaaagac ggcctcgaaa tccttgagaa aaatcccaat atcgcgctga
    20701 taatttccga tcaacgaatg ccagttatgt ccggaaccga atttctcaac cgttctatca
    20761 gcgttaatcc cgactgcatc cgcattattc tcaccggtta taccgatgtc aaagatttga
    20821 tcgaatccat caatgcgggg cgcgtattcc agtatgtgac caaacccttt gaaaccgatg
    20881 atctgcgcat tatcgtcaag cgcagcctgg atttttttgc ccaaagccgg gctctcgaac
    20941 gggctcatct tgaaatcaaa acggcttatg aaaatctgaa aaatgctcag gagcaactgg
    21001 ttcgctccga aaaaatgtcc atgttgggca aactcatgag cagcattgcc cacgagatcc
    21061 gcaacccgat aaacaatatc agcaattcga ccaaattgat ttcgctggaa tgggcggacg
    21121 tcaaaaatct ttttatcgaa gtcaacgcgc tcacgaccgg tcaaaagaca gccgaacaat
    21181 ttaagtcggc cgtcaaggat cgttataaca tcgaagaagt actgcaaaat tttgaaacgg
    21241 cagtcggtgt gatcaaacac tcgtgtgatc tggttacaga aattgtcgaa gatttgcgcg
    21301 gattttcacg gcttgatgat gccgaatttg ttcaaaccga tatacaccag cagatcgaac
    21361 gctcactcac acttctgaaa gcaaaattca agcatcacat cgaatttgta aaggattttg
    21421 gcgtcgtatc gcacattctg ggattaccgg ggccgctatc ccaagtattg atcaatctta
    21481 tcaataatgc ggcgcaagcc tcaccggcgg acggcaaagt caccatacaa acccgccaag
    21541 aaaataccac ggttcgtatt gctatcaccg acaatggcgc aggtattcct aaagacatca
    21601 tcggtaagat ttttgatccc ggatttacta ccaaaccgga aagtgaaggt accggtttcg
    21661 gcttatctat ctctgctgaa atcatcaaaa agcacaatgg ccggatcgaa gtcgtgtcag
    21721 ctcccggtat gggctcgaca tttactgttt atctgcccgt tgatccgcgc aacgtctctt
    21781 aagccatgca ccgcaactgg ttgatctccc gattaaacgc ataccatccg agcgatccgc
    21841 acgaatcggc gatgcgcgat cgtatgttga attttgtcca aacatacccc gattgttttg
    21901 aacgttatct gtccatcggg cacattaccg gcagcgcatg gatcgtcaac ccctcgcgcg
    21961 ataaagtttt actgacgcat cacggcaaac tcaaccgatg gctgcaatta ggcgggcatg
    22021 cggatggcga tccccatgta gatcgcgtgg ccttgcgcga agcgcaggaa gaatccggtg
    22081 tcgaagcgac aacgcttttg tccgatatgc cctttgattt ggatattcac gaaattccgc
    22141 cgcacaaatc ggacgcggcg catcttcatt acgacgtgcg gtttctgatc gaaatagatg
    22201 atgccatacc gctgcgcatt tcttccgaat ccaaagattt agcttggatt gcgttggatc
    22261 gcattgccga ttatacgaca gaagaatctg tactccgtat ggcgcgaaaa actgccgtaa
    22321 tgcaaaacaa tctttaacgt ttcttctttt tccagattaa cttcgaaatt agcgaggtcg
    22381 ttatgctgag taatcaagcg cttgaccctc attttcgctg gcgcgggcat gaagtcaccc
    22441 gtgtggaagc tttcagcgat gcggtatttg cttttgcaat gactttgctg gtcgtttcac
    22501 tcgaagtacc caaaaacctt tcccagcttt tggaaaccat gcgcggtttc ccggcatttg
    22561 ctacgtgttt tacgatcttg gtgatgttat ggtattggca ttacaaattt ttcaggcgtt
    22621 tcggtttaca agatcgtttt acgattgttt ggaatgccgc gctccttttt gtagtgctat
    22681 tcttcgttta tcccctcaaa tttgtcatga ccctcgtcgt caatatgatg ttgggcgtag
    22741 cttatgaaca gccgccgatc gcggcgggtc aaggcgacga catgatgtac atctacaatg
    22801 caggatacat cgccgtgtgc ggtatctatg ccttgctgta ccaccatgcg taccgtcttc
    22861 gcgcgcgatt tgaacttaat gaagcagagg ttttcattgc ccgttccgaa cggcgtgcgt
    22921 atatcatcaa tatcggtatt gcgctttttt caacctcttt acttttatac ggcggctccg
    22981 ctatggcc
//
LOCUS       DAOQGF010000046        22733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1044_length_22733_cov_7.124041, whole genome shotgun sequence.
ACCESSION   DAOQGF010000046 DAOQGF010000000
VERSION     DAOQGF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22733
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_22733_cov_7.124041"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PK937_08070"
     CDS             complement(<1..1055)
                     /locus_tag="PK937_08070"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNH32565.1"
                     /translation="MFYVESIYSDIRLRENAERISEHIIQGLWNYLFLRMDDLTTVDG
                     HKIRILDKGRWHSGSGPDFRQATLRIGDTILCGDVEIHWQTTDWLKHGHERDPAYFRV
                     ILHVVYRHDKDLHQGAPYTLEIKDRLSDTLKTLTEKVMLLDADQDALFCSDFVPSIEP
                     EFVTQWVQENGAHRLRRKTEQLKNLQKEKQWTDEQLLYYVLCDAMGFTKNRLPFCMLA
                     ERVPYDVVYASANGVDSSVALMRVQALLIGAAGLLPDEATMSADPKLWPYAGRLWDLW
                     SALSSQHQITPLHAEVWIRDRLRPKNYPLPRMAALAGLLVTHLKDGLSVAVIRLFSQC
                     DTPPVILKNLHRILKVRA"
     gene            1122..1207
                     /locus_tag="PK937_08075"
     tRNA            1122..1207
                     /locus_tag="PK937_08075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1156..1158,aa:Leu,seq:caa)
     gene            complement(1436..2572)
                     /locus_tag="PK937_08080"
     CDS             complement(1436..2572)
                     /locus_tag="PK937_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HNH32566.1"
                     /translation="MIKAQVSTFDDWIDLFQEWKKDIGYDEKTFKRFLPNYKLEPKYE
                     ALKYNEIEFGDFRGDRKWERVLDIPNQGIRDALQQLIVFQGDTEFASVEQQRYLVDRA
                     PSDYDLYALMRINAEEMRHGWQMSHILVTHFGDAGRKEAMKLLERRAFEQKRLLGSFN
                     EVMENWLDLYTYTEFIDRDGKFQLKMLSHSAFMPLSMSMNPMLKEESFHLGTGNNGLK
                     RIIKAGKIPVEIIQKYFNKWVPTAYDLFGTDNSSNAHWAYVWGLKGRFNEHETDEEVQ
                     KDKLNQLARDLYIAEISKLVEDMNTLIPREMPKLIVPDVKFNRSIGLHAKQPYTVDGR
                     LLSEEQYKTYRETVLPTDKDRELLKDIFKENDWIEFKPFKGAEA"
     gene            complement(2608..3306)
                     /locus_tag="PK937_08085"
     CDS             complement(2608..3306)
                     /locus_tag="PK937_08085"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH32567.1"
                     /translation="MNTAVTKSSVRSTVPKRRLYMPENAGDQARFHLRLNDILERSAT
                     LFSEQGYHNTSMRDIAGAMHTSLAGLYYYFKTKEELLFLISQYSFNSVIGSLHEKLAH
                     VHDPVLKLHILVQNHLLYFVTHLDAMKILALESDSLTGEYHRIINEKKKNYLKILETI
                     LSEMVISKNNKSARRTTPEIKIAALALFGMMNWTYTWYNPKKRTHNTTNIARISKQMV
                     DIFLSGFLQKNLQS"
     gene            3502..4305
                     /gene="rsmA"
                     /locus_tag="PK937_08090"
     CDS             3502..4305
                     /gene="rsmA"
                     /locus_tag="PK937_08090"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNH32568.1"
                     /translation="MSSVAVKTPAPKRKFSQNFLTNPHYVRKIVDAANLSSGDTAVEI
                     GCGKGALTELLLESGATLHGVEIDPDMIRILTERFSQHPRFTLHHQDILTFDFHGMAS
                     GFPLTVIGNLPYHISSPILFHLMESGPIIRQALIMVQKEVAERIVGKPHSKDYGILSI
                     FCQYHAACEKLFDLPPGAFFPAPKVTSSIVRMTFHRDMTPWPHYPLFVKIVKTGFNQR
                     RKMLRNSLSSFEGINNVTIDLSQRPENLSVEDFIRLTDELAMHQRHYAA"
     gene            4295..5656
                     /gene="mgtE"
                     /locus_tag="PK937_08095"
     CDS             4295..5656
                     /gene="mgtE"
                     /locus_tag="PK937_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNH32569.1"
                     /translation="MRPKAEIEIDKELIDDIKDLVDSKAELLVRNILSDLHPSDISEI
                     LDHLDEEERNYVFQVIEPEKASEVILELNATSRENVLDNLDEEQITEIIEEMQTDDAA
                     NIVQELDDETAKRVLDQLDYEDQQEVKELLKYDEDTAGGLMQLEFVSVIDTSTMVQAT
                     NEARKKAADVEKIHDIYVVNSYGHLVGSISIEKLLVEPAEKHVAEVMHQEIPFAKTDE
                     DQEEVAHLFKKYDLIALPVVTRAGKLVGRITIDDIVDVIEQEASEDVSKMAGSVDEDV
                     SEQSTFRISKSRLPWLFTALAGETVAAFVLSRFESSLNAILALAFFVPIIMAMAGNIA
                     IQCSSIVIRGLATGEISIIDIQRQIFKELRVAAINGFVLGIALAMIVALWIGNMQVAF
                     VVGIALICVIFFAAFNGTLFPLLLKKLNIDPALAAGPFVTMTNDTIGILIYLGIATLL
                     LIR"
     gene            complement(5670..6041)
                     /gene="acpS"
                     /locus_tag="PK937_08100"
     CDS             complement(5670..6041)
                     /gene="acpS"
                     /locus_tag="PK937_08100"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNH32570.1"
                     /translation="MIAGIGTDIVEISRIRQSCEAYGDRFIHRVFTPGEITYAYSKKD
                     PYPSLAVRFAVKEALIKAARVGKFHPHGWLDAEVITDARGVPSIELYGELKKELAGQQ
                     IHITVSHADHYATATVIIEKK"
     gene            complement(6038..6670)
                     /locus_tag="PK937_08105"
     CDS             complement(6038..6670)
                     /locus_tag="PK937_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNH32571.1"
                     /translation="MSATPTALTPELTLYCRLSACVAIHDAEPMRESIATALDAGYDV
                     LRIKEVILQNYLFSGFPSAIEGLIVLGATLKHRGIADNNWVEMRTEDQIKSDGMALCA
                     MIYDKNFDKLMHNMQSLSADIRDWMIFEGYGKVLSRPILTAKERELCVISALAAMGRE
                     RQLISHLRGAVHVGASDGEISEALQSISQTAARGKISEAMRLWDETRSRS"
     gene            complement(6680..7921)
                     /locus_tag="PK937_08110"
     CDS             complement(6680..7921)
                     /locus_tag="PK937_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32572.1"
                     /translation="MFNRIGKEQDSPESRAKLKVLCVDDEYVITNIMERILDEHYQVF
                     TASSGFEGLDILKQHPDTVVIVSDQKMEGMTGNEFLMKSQEIAPEAMRIMVTAYSDID
                     LVIDSINKGQIYKFIPKPFDAEHFRVTIHRAVDHYHHKIAYEKAFRELQTAQEGLVRA
                     EKLSALGKLLSDVSHELGGPIANINHAVTLAQHEWKDLKEFLDSVTSANFSAIDVQLL
                     MREKRVAELTHDFEQMMETIANASVFAMDLLSELKGFARNDDQQWIAVDIEKQIERAL
                     HLLRIKYKNKITFHKDYTPLPSIKGLPGPLTQVMVNLLQNSAQSIQKEGHIWIRTWRG
                     PDGIRVSIKDDGQGIAEEHISQVFDPGFTTKPEAEGTGFGLAISQAIIQKHGGSIAVT
                     SVLGKGTEFIISLPWEQALSA"
     gene            complement(7932..10352)
                     /gene="lon"
                     /locus_tag="PK937_08115"
     CDS             complement(7932..10352)
                     /gene="lon"
                     /locus_tag="PK937_08115"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNH32573.1"
                     /translation="MPEANISTDMKSDSIAIPEKLPILPLRDNVIFPFVVFPLVVMEK
                     NHVQLVQEALSSHKIIGVFTLRQPDKENPSFEDLYGIGTAVNVLKMFQVPDGSVRLLI
                     QGFSRIRLKAATPDGLYLSGQVEPIHVPSADDLETDALKRGVSESFQKMVTLSPILPD
                     ELKVIVSNIREPDKLADMVASSLNIELNEKQEILESIGVKERLTKVMSAISREIQLLE
                     LNNKIQTEVNAELSKNQREYFLREQIKAIRHELGEDDDGSVEVEELKAAVEKKKMPAE
                     AKEAAMKEISRLSQMSPSAAEYTVSKTYVDWLLELPWYNATRDRIDLKKAKKILDEDH
                     YDLDDVKNRILEFLAVKKLRKDSKSPILCFLGPPGVGKTSLGRSIARAIGRKFVRFSL
                     GGVKDEAEIRGHRRTYIGALPGKIIQEIRRCQSSNPVFMLDEIDKIGQDFRGDPSSAL
                     LEVLDPAQNGSFNDHYVDIPFDLSDVMFIATANEISPIPAPLLDRMEVIRLSGYIAED
                     KLQIARKYIIPRQIEENGLKESDIEITDEAVYQIIASYTWESGVRNLEREIANVCRKI
                     ARKKAEGNKNKAKVQLPDIVEYLGPQKVFPEVARRTSETGVATGLAWTMNGGEILFIE
                     ARLMQGNRGMQMTGQLGNVMKESAQAAISYIRSKAGDLGIDPKIFDKNEIHMHIPSGA
                     TPKDGPSAGVTIVVCLTSLLTGKKVRHDIAMTGEITLRGKVMPVGGIREKVVAARRAG
                     IKTVILPKLNKNDLDDVPEYVKSSLTFIFADHIDDVLPHVFENDRTKKKSRASHKRSS
                     ATKRRRKG"
     gene            complement(10362..10817)
                     /locus_tag="PK937_08120"
     CDS             complement(10362..10817)
                     /locus_tag="PK937_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH32574.1"
                     /translation="MDDMEDDKKYSIHRLRGVDDDMDFIFSNLFNTKFPILLHSEKRW
                     HPPTDVLETDDEFIVVMDIANVKQDDIRLTYEAGILTVSGHRHEMKFSEKRHYHKMEI
                     DFGPFERRIKVNTRIQDQAIKAGYENGFLIVRLKKDTSRSSRVTNIVIE"
     gene            11062..12066
                     /locus_tag="PK937_08125"
     CDS             11062..12066
                     /locus_tag="PK937_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH32575.1"
                     /translation="MPTVAAWGQLRLELGFDRKNNLLWTTDPGIEKAAYSNLDIETWG
                     DFKLRNDLPQSPGGDGYLSLNGIQQYFQIPERAGLQVSGNISFSISMWLRVPAGTQNG
                     DIISSDNGFINGYRLFLEDGMIRFEIRDGTKEIFSSNRLLMPNQWQHIGVVCDGLGDS
                     VTFFINGASVASHPFTRVVQVQSGTQTYIGTRAESSFPNYLYGDIDDFRFFSGHDTIF
                     EYIRTMPERDLQRLSLKRPKTTTPSYFTLYQNYPNPFNMATRIDFEMIRPGSAELQIF
                     DLLGNMMKPIFTGELSAGRHTYTWDGTDNNNTPVSSGIYFARLTVNGIVQTKKMVLIK
                     "
     gene            complement(12070..12759)
                     /locus_tag="PK937_08130"
     CDS             complement(12070..12759)
                     /locus_tag="PK937_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH32576.1"
                     /translation="MTNKSQILLVEDETHIAESLQFNLETEYNVIWAQTGEDALVQWK
                     KSKFDLIVLDVMLPKKSGYDVCAEIRKTDSETAILFLTAKGDARDRIEGLKLGADDYL
                     GKPFELDEFLLRVKALLKRRNAVNESSTSKFSFDKNEIDFENYIAVARGKKIQLTPKE
                     CMLMKLLIERQEQVVTREEILMKVWGYEAIPSTRTVDNFIVRLRNYFEVNPKDPTHII
                     SVHGVGYKFVP"
     gene            complement(12773..14038)
                     /locus_tag="PK937_08135"
     CDS             complement(12773..14038)
                     /locus_tag="PK937_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH32577.1"
                     /translation="MKLQKKTIVIFIVIVLLCLAQIFWWMYFQVQQNRTVMNLHKKMI
                     SSKALELRRFLTLEYRDLIQGSLNHPLHILRQAQENDWPRSHFVVTPDTIFLRYQDLI
                     YFFPINWPFLEQKLAASDSGFVLQKKPTISSAPATYLELPLTVIPKYKAEDNLQEQMD
                     RRTVMFLSEGSFFALMVMIAIYFLYAAFRRELQLQEHQKNFILSMTHELRSPLSSIKL
                     YLQTLQRRQITEERREQFVQHSLADVERLQRLVENVLDAARIERNDYDYQMGSLDMSQ
                     LVKSVLDKWKTSDTESVISFSGNIASDIHIHADAHAMISVVNNLIENAVKYSQAPKRV
                     NVLLSMIGDRAVLTVTDNGPGVAPEDRTKVFERFYRAGDEMTRTTKGTGLGLYIVKRI
                     VEAHRGTIDCQSAMEGGAAFTVSIPTLKK"
     gene            complement(14047..16368)
                     /locus_tag="PK937_08140"
     CDS             complement(14047..16368)
                     /locus_tag="PK937_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNH32578.1"
                     /translation="MVQRPYTVASDGTGRYRTIQEAVDKSELNSIIRVKAGIYRENLI
                     LRNFISLEGDPAGRTLVIGSPTAPVIQAYNISSLRLRDLSFEFDQPTAFPVLLVRYSN
                     FSADRVAFRRGATGVEMKSNSTVTLRECSISDNTGYGISVSDRSQGTITQSFIYQNEA
                     SGISVSDKSSPLIERNIIRDNKKDGLLINLGSVGKVIGNTIYRNGENGITIDGQSNPL
                     IRNNTIVFHKNDTSRYAEKETGYGVLIRNASGLALINNNIVWNHIGIGQKQGTLALLS
                     NNNVWNNGVLYEGIEAHHTDVSHDPMFLNAAISDFRIDTTSKIYMAGEDRLSIGSHYD
                     YVRSDKKTRLDYLKTQAAKDLAKENWYQAFQNAKEILEIDKTDTEGKSLHKRAASQLA
                     DMYTKNARTEYEKDSYRIARGFLDMALGFDPEFADALELKQEIEEESRWINIRFTFVA
                     VSGCLVLAVLIFYMRKRSRRQELRRQAQWWVDDADENIVLARTAEAEMIASEDFKSAL
                     DKFSALQKAFAEMQYGLCESLSAEVVRLAIRAKDSAERYKQLRKEALLEVSNAELDLK
                     NFSESEDGVSFPKEISEYRFYLERAQDALVQKHYVMAKEIADDIQRNTRDLRQRLQTQ
                     RETAVITLIQETETCIIEALAGHSSADIIMAVMDFRSELQVLKSGFENGQLSVAEVTP
                     QVEQIKSFVEEAMRLGGVYPSSASKAHRSYYDILGIKEDASIDQIKAVYRKLSMIYHP
                     DANAADDTGIVGDERFKEIKAAYEALLKDKQTL"
     gene            complement(16574..17872)
                     /locus_tag="PK937_08145"
     CDS             complement(16574..17872)
                     /locus_tag="PK937_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH32579.1"
                     /translation="MNQPVPIQIPLVTLSLHQKISLALVVIGFLMLGVALSPISEQYP
                     IGMFLLSSGLITIGALAYIMPMWYDRKPGIKHDRIFFNSFVNRGVWGWIAAVFFTGFY
                     VVLYWFPERLEYLIHTTDWLSGILRNKPADQWFLYGFYYTLAVMVMGLRMMAKNRHSR
                     YQLIRTVSVIFFQLGFAFLLPGLLQKFNQPEFYFSYFWPLKYSYLFPNDINWLISQPG
                     GLGTFMVFWGAVMSFIATPILTYFFGKRWYCSWVCGCGGLAETLGDPYRHLSDKSLRS
                     WKIERYSVYGVLIFVVITTAVVWINSLSNGQLLGNVSSLFSNVYGFVIGALFSGVIGV
                     GFYPLLGSRVWCRFGCPMAAYLGILQRYFSRFRITTNGGQCISCGNCSTYCEMGIDVR
                     SYAQRGQNIVRASCVGCGICSAVCPRGVLNLENGPIHSRQ"
     gene            complement(17902..19233)
                     /locus_tag="PK937_08150"
     CDS             complement(17902..19233)
                     /locus_tag="PK937_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH32580.1"
                     /translation="MSQIIIIGNGITGTTVARHVRKRSDQPITIISGESDYFFSRTAL
                     MYIYMGHMKYEHTKPYEDDFWKKNEIQLRRGLVERIVPAEKQIYLKNGEAMKYDKLVI
                     ATGSVYQTFGWPGQNLQGVQGLFSLQDLELMERNTKSVKRAVIVGGGLIGIEMAEMLI
                     SRGIHVTFLVREKSYWDNILPAEESDMINRHIRAHHVDLRLNTELQEIRGNDKGRVDA
                     IVTKSGEVIPCEFVGLTVGVKPNIEFLRSSGIPLNRGVLINQFLETPIADIYAAGDCA
                     EFIEPKPAHPKIEQLWYTGRMQGEALARVLTGERQAYDRGIWFNSAKFFDIEYQTYGV
                     VPNVPRDGEEHLWWEHPDGQHGIRIVFRKDNRIVIGFNLMGIRYRQNVCQRWIKESRT
                     IDYVLQNLGEANCDPEFFMQYEPHIIAEYNRKYPEQNLLLQVQRGLHFIVR"
     gene            complement(19254..19433)
                     /gene="rpsU"
                     /locus_tag="PK937_08155"
     CDS             complement(19254..19433)
                     /gene="rpsU"
                     /locus_tag="PK937_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNH32581.1"
                     /translation="MLSTIVRKNEPIEKAIRRFETEVRKARIIQTCIEKSNYVSPSER
                     KHIAHKRKKRNTGNA"
     gene            complement(19540..21837)
                     /locus_tag="PK937_08160"
     CDS             complement(19540..21837)
                     /locus_tag="PK937_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH32582.1"
                     /translation="MIVRYSLFLLCAAGVLYSQNYFKDTEALTFYDQDGMIPYASHGG
                     MYVPKTEFADIDSDGDPDLFIVQTDGRITHYMNSGVEGPLRWLWITDNFASLEVNQWV
                     RFIDADADGDLDLFCGVSGARMGYYKNEGSAVNAHFVLQTETVLDTLNAPVLTEDQTV
                     PVFKDIDSDGDYDFFSGRSVGTLSYYENVGNEHSFLLKFITDAFQDIAIITPAKQSEE
                     QRHGASAVCFFDIDDDRDPDLFWGDFFSTGLYFLPNLGDSAHPFIPDTATSTYPSRQL
                     KTLGYNMPSFVDADGDGDGDLLVSVLYREEDRDNLWYYRNIGTPQAAQFQLITKNLLQ
                     NIDVGRNAHPIMADLDNDGRLDIILGSYSGAIHFYRQTVNGVFQSNSSWFVTIPQDEF
                     ITAPALGDIDNDGDLDLFVGKFDGKIMFYRNTGSLTSPEFTLENQFYANLDIGNNSSP
                     FLSDFEGDGDLDLIIGEESGEVQLISNNGTLSIPDFSTATVTLIAANGLSDAVPILLD
                     WDGDSDRDLIVGYKNGTLRYYERQNANSFQEIAGKFSTIKVTGQAVPFALDWDNDGDK
                     DIVCGNLRGGIEFYRAGTQKPIIKIPNKQKIRSDSSWTYRLIANANPAPRWQILSGPS
                     SLKCDSISGIVQWKPVVTDIGTHVVEVRAYNIDGADTAKFEINVSVYEESPLQLLYNS
                     PNPFDERTSIVMKINKTMEVKAYIYDVRGGLVHRLVSGQLSPGIYKREWNGKNTHGNK
                     VASGIYFVTVVANGKTYSRKILFIK"
     gene            complement(21852..>22733)
                     /locus_tag="PK937_08165"
     CDS             complement(21852..>22733)
                     /locus_tag="PK937_08165"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HNH32583.1"
                     /translation="LPLCSCSVVPVGISLKKNGASNGATMSFLVSTPETGVDSIAVSY
                     AFLDPIMTIIRPIAAFITAIVSGSLENIFGSQKQSDTVQQSDAPQTSCNCSTNCNNEA
                     DKTVESRFDRLKSGIVYAFTNLLGDVAYYLLLGLIASGILSAIIPENFFIDVMHSPLV
                     NMMIMLAVGIPIYVCASASTPIAAALIMKGITPGAALIFLLAGPATNISTIALVRSKL
                     GQRSLIIYLFSIAMIALAMGSLTDSVYSYFDIKPQVSIGTMHDVIPESIHIISAVIFL
                     PILLLACFREIRHRFFK"
BASE COUNT         6149 a         5389 c         4767 g         6428 t
ORIGIN      
        1 gcacggactt ttagaatacg atgtaagttt ttaagaatta ccggcggcgt gtcgcattga
       61 gaaaatagtc gtatcacggc aacgctcaaa ccgtctttga gatgagttac aagcaatccc
      121 gcaagagcgg ccatgcgcgg aagaggataa tttttcgggc gaagccggtc gcgtatccaa
      181 acctcggcat gcaacggagt aatttggtgc tgcgaagata aggccgacca caaatcccat
      241 aatcgccctg cataaggcca caacttggga tcggctgaca ttgtcgcctc atccggaagc
      301 aaaccggccg caccgatcag tagcgcttgc acacgcatca gtgcgaccga tgaatctaca
      361 ccatttgccg aagcatacac cacatcataa ggcacacgtt cggctaacat acaaaaagga
      421 agcctgtttt ttgtaaagcc catcgcatca cacagaacat agtagagaag ctgctcatcc
      481 gtccactgtt tttctttttg taagttttta agttgctcgg ttttccggcg cagccgatgc
      541 gcaccatttt cctgcaccca ttgcgttaca aattcaggtt cgatggacgg cacaaaatcg
      601 ctgcaaaaaa gagcatcctg atccgcatcc aatagcatga ccttttccgt caacgttttc
      661 aaagtatcgc tcaggcgatc tttgatttcc agcgtgtagg gcgccccttg gtgcagatct
      721 ttatcgtgcc tatacacgac gtgtaagatc actcgaaaat aagccggatc gcgctcatgg
      781 ccgtgtttga gccagtccgt agtttgccag tgaatttcta catcgccgca caatatcgta
      841 tcgccgatgc gcaaagtcgc ctgacgaaaa tccggcccgc tgccgctgtg ccatcgccct
      901 ttgtctaaaa tgcgaatttt gtgcccatcc acggtcgtca ggtcatccat acgcaaaaat
      961 aagtaattcc ataaaccctg tatgatgtgt tcggaaatgc gttcggcatt ttcacgcaac
     1021 cgtatgtcgc tataaatgga ctcgacgtaa aacataaaaa atctgttgca aaaaatggaa
     1081 aactggctat attcatgcca cgttttttga aacgatagaa cgccgaagtg gtggaattgg
     1141 tagacgcgct ggactcaaaa tccagttggg ctcaaaccca gtgagagttc gagtctctcc
     1201 ttcggcaccc caaagccatg cataacactg tgacgcatgg cttttttatt tctctcgatt
     1261 aaaaacctcg catcgtattt attataagat acaccacgat agtttggatt ttatgtcaat
     1321 ctctgacaaa aaaagcctta aagttcattt aagacgcagt atcgaaacga cagcccaata
     1381 aaaaaaaccg cgcggtctcc cgagcggttt tttgacgaaa aaaatatgaa tcggatcagg
     1441 cttccgcacc tttgaaaggt ttgaattcga tccaatcatt ttctttgaaa atatctttca
     1501 atagctcacg gtctttatca gtgggcaaca ccgtttcgcg ataggtcttg tattgctctt
     1561 cactcaaaag acgaccgtcc actgtgtacg gctgtttggc atgcagaccg atgctgcggt
     1621 taaattttac atcgggcacg atcaatttgg gcatttcgcg agggatcaga gtattcatgt
     1681 cctccacgag tttgctgatc tcggcgatat acagatcgcg tgcaagctga ttgagtttgt
     1741 ctttctgcac ttcttcatcc gtctcatgtt cattaaaacg acctttgaga ccccagacat
     1801 aagcccagtg cgcgttggac gaattatccg ttccgaaaag atcgtaggcg gtaggaaccc
     1861 atttgttaaa atatttttga ataatctcga ccggtatttt acccgctttg atgatacgct
     1921 tgaggccgtt attacctgtg ccaaggtgaa atgattcttc tttgagcata ggattcatac
     1981 tcatcgagag cggcataaat gccgaatggc tcagcatttt gagctgaaac ttaccgtcac
     2041 gatctatgaa ttcggtatag gtgtaaagat cgagccagtt ttccatcact tcattaaatg
     2101 aacccaataa ccgtttttgt tcaaacgcac ggcgctccag gagtttcatc gcttctttgc
     2161 ggccggcatc gccgaagtgc gtcactaaga tgtgactcat ttgccaaccg tggcgcattt
     2221 cttccgcgtt gatacgcatc agcgcataga gatcgtaatc gctgggcgca cgatccacca
     2281 gataccgctg ctgttcgacc gaggcaaatt ccgtatcgcc ttggaatacg atcaactgct
     2341 gcaacgcatc gcgaataccc tgattgggta tgtccagcac gcgttcccac ttacgatcac
     2401 ctctaaaatc gccgaattcg atttcgttgt acttcagcgc ttcgtacttg ggttccaatt
     2461 tgtagttggg gagaaaccgc ttaaatgtct tttcatcata gccgatgtct tttttccatt
     2521 cctggaaaag atcaatccaa tcgtcaaatg tggaaacctg tgctttgatc ataacgcgac
     2581 ctttcggtta agagttattt atgtgaatta tgattgtaaa ttcttttgta agaaaccgct
     2641 cagaaaaata tccaccatct gcttggaaat acgggcgata ttggtggtat tgtgggtacg
     2701 ttttttgggg ttgtaccacg tataagtcca gttcatcata ccgaaaagag ccaacgcggc
     2761 aattttgatc tccggcgtcg tccgtcgggc agatttgttg tttttggaga ttaccatttc
     2821 ggagagtatg gtttccaaaa ttttaagata atttttcttc ttttcattaa taatgcgatg
     2881 atactctccg gtcagcgaat cgctctccaa tgcgagtatt ttcatagcgt caaggtgcgt
     2941 gacaaaatag agcaaatgat tttgaacgag gatgtgcagc ttcaaaaccg gatcgtggac
     3001 gtgtgcaagt ttttcgtgca acgaaccgat aaccgagtta aatgagtact gcgaaatcaa
     3061 aaacaaaagc tcttctttag ttttaaaata ataatacaaa ccggccaaac tcgtatgcat
     3121 cgctccggct atgtcgcgca tggacgtatt atgatatcct tgctcggaaa aaagagtcgc
     3181 tgagcgttcc aggatgtcgt tgagccgcag atgaaatcgc gcctggtcac ctgcattttc
     3241 aggcatatat aaccggcgtt tgggcacggt actgcgtacg gatgatttgg tgacggccgt
     3301 gttcataaat caggtttgat tcggttatac aattcgaacg aacgttcgaa atctaagcga
     3361 ccgtcggcat attgtcaaat ttattttttt acggcaatcc cgtcgtttat tttatccgtt
     3421 catgactttc ccgttgaaac ttcctgtcat gattcgtata tattcaaaat acatctccat
     3481 tttccaaatt tcattttttt catgtcttcc gttgccgtca aaacgcctgc acccaagaga
     3541 aaattcagtc agaattttct gaccaatccg cattatgtgc gaaagatcgt ggatgcggca
     3601 aatttatcct ccggcgacac cgcggtcgaa atcggatgcg gtaaaggtgc gctgaccgaa
     3661 cttttgctgg agagcggcgc gactcttcac ggtgttgaaa tcgatcccga tatgatccgt
     3721 attctcacgg aacgtttttc ccaacatccg cgttttacgt tgcatcatca ggacatcctt
     3781 acttttgatt ttcacggtat ggcttccggt tttcctttga ccgttatcgg caatctcccg
     3841 tatcatattt ccagtccgat tttatttcat ctgatggaat ccgggccgat catacggcaa
     3901 gctctgatca tggtacaaaa agaagtcgcc gaacgcatcg tgggcaaacc gcacagcaaa
     3961 gattacggca ttctttccat tttttgccaa taccatgcag cttgtgaaaa actttttgat
     4021 ttgccgcccg gcgctttttt tccggcaccc aaagtgacgt catccattgt acggatgacc
     4081 ttccatcgcg atatgacgcc ctggcctcat tacccgcttt ttgtaaaaat tgtcaagacc
     4141 ggatttaatc agcgtcggaa aatgttgcgt aattcccttt cttcgtttga aggcatcaac
     4201 aacgtcacga tagatttgag tcaacgcccg gaaaatcttt cggtagaaga ttttatccgg
     4261 ctaaccgatg aacttgctat gcaccagagg cattatgcgg cctaaagcgg aaatcgaaat
     4321 tgacaaagaa ttgattgacg acatcaaaga tctcgtcgat tccaaagcag aacttcttgt
     4381 tcgtaatatt ctgagcgatc tgcacccgtc cgacatctca gaaatcttgg atcacctgga
     4441 tgaagaggaa cggaattatg tttttcaggt gatcgaacct gaaaaagcct cggaagtcat
     4501 tcttgaactg aatgctacgt cgcgcgaaaa cgtactcgat aatctggatg aagaacaaat
     4561 caccgagatc atcgaagaga tgcaaacgga cgatgcggcc aatatcgtac aggaactcga
     4621 cgacgaaacg gccaaacgcg ttctagacca acttgattat gaagatcagc aggaagtaaa
     4681 ggaactgctg aaatatgacg aggatacggc cggcggtctc atgcagcttg agtttgtatc
     4741 cgtgattgac accagtacga tggtgcaagc gactaacgaa gcgcgtaaaa aagcagcgga
     4801 cgtcgaaaaa atacacgata tctatgtcgt caatagctac ggtcacttag tcggttcgat
     4861 ctccatcgaa aaactactcg tggaaccggc agaaaaacat gtcgccgaag taatgcatca
     4921 ggaaattccg tttgccaaaa cagacgagga tcaggaagag gtcgctcatt tattcaaaaa
     4981 atacgatctg atcgctttgc cggtcgtgac tcgtgccggt aaactggtgg gtcgtatcac
     5041 cattgacgat atcgtggatg ttatcgagca ggaagcttcc gaagacgtct ccaaaatggc
     5101 tggctccgtg gatgaagatg tcagcgagca atccacattt cgtatttcta agtcgcgttt
     5161 gccttggctt tttacggcac tggccggtga aacggttgcc gcttttgttt tgagccgatt
     5221 cgaatcgtcg ctcaacgcaa tattggcatt ggcatttttc gttccgatca ttatggcaat
     5281 ggccggcaat atcgccatac aatgctcatc catcgtcata cgtggtctcg ccaccggtga
     5341 aatcagcatc attgatattc agcgccaaat attcaaagag ctgcgcgttg cagccatcaa
     5401 cgggtttgta ttgggtattg ctttagcaat gatcgttgcc ttgtggatcg gaaacatgca
     5461 agtggccttt gtggtgggta ttgcactgat ctgcgttata ttttttgccg ccttcaacgg
     5521 caccttattc ccactattgc tcaaaaaact caatattgat ccggctttag ctgcaggacc
     5581 ttttgtcact atgaccaacg atacgatcgg tattctgatt tatctgggta tcgcgacact
     5641 gctgctgata cgatagaaat tttgaagcat tacttttttt cgatgatcac ggtcgccgtg
     5701 gcataatgat cggcatgcga aactgtaatg tgtatctgct gtcccgccaa ttcttttttt
     5761 aattcaccat aaagttctat cgaaggcaca ccgcgtgcat ccgtgatgac ttctgcatcc
     5821 agccatccgt gggggtgaaa tttacccacc cgcgccgctt tgatcaacgc ttctttgacg
     5881 gcaaatcgaa cggccaacga cggatacgga tcttttttag aatacgcata tgttatttct
     5941 cccggggtaa atacccggtg aataaaacgg tccccatacg cttcacaact ctggcgtata
     6001 cgcgatattt cgacaatgtc cgtgcctatc cctgcgatca tgaccgactc cgtgtttcat
     6061 cccataggcg catcgcctca gaaatttttc cgcgtgcggc ggtttgtgaa atcgattgaa
     6121 gcgcttccga aatctcgcca tcgctcgctc cgacgtgtac ggcacctcgc agatgggaaa
     6181 tgagttgcct ttctcgaccc atcgccgcca gcgcactgat cacgcacagc tcgcgttctt
     6241 tggccgtcag tatcggacga ctaaggactt tgccgtatcc ttcaaaaatc atccagtctc
     6301 ggatatcggc gctgagcgat tgcatattat gcataagttt atcaaaattt ttatcgtaaa
     6361 tcatcgcaca caaggccata ccgtccgatt tgatttgatc ttccgttcgc atctctaccc
     6421 aattgttatc ggcgataccg cggtgtttga gcgtcgcgcc aagtacgatc aaaccttcga
     6481 tcgctgatgg aaatccgctg aaaagataat tttgcagtat cacttctttg atgcgcaata
     6541 cgtcataccc ggcatccagt gccgtagcaa tgctttcgcg catcggctcg gcatcgtgta
     6601 tggcgacgca cgccgatagg cggcaataca gagttaattc gggagttaaa gcggtaggcg
     6661 ttgcactcat aaccgttatt tatgcgctta gcgcctgttc ccacggcagg ctgatgataa
     6721 attccgtacc cttacctaaa acactggtta cagcgatcga accgccatgc ttttgaataa
     6781 tagcctgcga aatagcgagt ccaaatcccg tgccttccgc ttccggtttt gtcgtaaaac
     6841 ccggatcgaa aacctgagaa atatgttctt cggctatccc ttgcccgtca tctttgatgg
     6901 atacgcgaat gccgtccggt ccgcgccagg tgcgaatcca aatatggcct tctttctgaa
     6961 tagactgcgc cgagttttgt aaaagattga ccataacttg cgttagcggt cccggtaaac
     7021 ctttgatgga cggtaacgga gtataatctt tatgaaaagt tattttgttt ttgtatttga
     7081 tgcgaagaag atgcagagcg cgttcgattt gtttttcaat atccaccgcg atccattgct
     7141 gatcatcatt acgggcaaat cctttaagtt cgctcaataa atccatcgca aaaacggacg
     7201 cattggcaat cgtctccatc atttgttcaa aatcatgcgt gagctctgct acacgttttt
     7261 cgcgcatcaa aagctgaaca tcaatcgccg aaaaattagc cgacgtgacg ctatccaaaa
     7321 actctttcag atctttccat tcatgttggg ccagtgtcac ggcatgattg atattggcta
     7381 tcgggccgcc cagttcgtga cttacatcgc ttaaaagttt gcccagcgcg gataattttt
     7441 ccgcacggac tagcccttcc tgcgcggttt gcaattcacg aaaagctttt tcgtaagcga
     7501 ttttatgatg gtaatgatct acggcacggt gaatggtcac acgaaaatgt tcggcatcaa
     7561 acggtttggg aatgaactta taaatctgac ctttattgat cgaatcaatc accaaatcaa
     7621 tatccgaata ggctgtaacc atgatacgca tggcctcggg agcaatttcc tgtgacttca
     7681 tcagaaattc gttgccggtc atgccttcca ttttttgatc ggatacgatc acgacggtat
     7741 ccgggtgttg ttttagaata tccaagcctt caaaaccgct gctcgcagtg aatacctgat
     7801 aatgctcgtc gagaatacgc tccatgatat tggtaatcac gtactcatca tccacacata
     7861 gaactttgag ttttgcgcgg ctttcaggcg aatcttgttc tttgccaata cgattgaaca
     7921 taatacgtgt actatccttt gcggcgtcgt tttgtagcag atgatcgttt atgcgaagcg
     7981 cgtgattttt ttttagtacg atcgttttca aatacatgcg gcagcacgtc gtcaatatga
     8041 tcggcaaaaa taaaggtaag cgatgacttc acatattccg gtacatcatc aagatcgttt
     8101 ttattaagct ttggtagtat cacagtctta ataccggcgc gccgggcggc gaccactttt
     8161 tcacgaatac cgcccacggg cattaccttt ccgcgtagag taatttcacc ggtcatggca
     8221 atatcatggc gaactttttt tccggtaagc agcgaggtca ggcacacgac gatcgtcaca
     8281 ccggccgacg ggccgtcttt gggtgtagct cccgacggaa tatgcatgtg tatttcattt
     8341 ttatcaaaaa tcttgggatc aatacccaag tcaccggctt tggaacgaat atatgagatc
     8401 gccgcttgcg ccgattcttt cataacattc cccaattgcc ccgtcatctg cataccgcgg
     8461 ttgccctgca taaggcgcgc ttcgataaaa agaatttcgc cgccgttcat agtccatgcc
     8521 aaaccggtag ctacacccgt ctccgaggtg cgtcgcgcca cttcaggaaa tactttttgc
     8581 ggaccgaggt attcgacgat atccggcaac tgaactttgg ctttattttt attaccttca
     8641 gcttttttac gagctatttt gcggcaaaca ttggctattt cgcgttccag attacgtacg
     8701 cccgactccc aggtatagga tgcgatgatc tgatacaccg cttcatcggt tatttcaata
     8761 tcgctttctt tcaatccgtt ttcttcgatc tgccgcggga ttatatattt tctggcaatc
     8821 tgcagcttgt cctccgcgat gtaacccgac agacgaataa cttccatacg atccagcagc
     8881 ggcgcaggta tcggggaaat ttcattggcc gtagcgatga acatcacatc gctcagatca
     8941 aaaggaatgt caacatagtg atcgttaaat gaaccgtttt gtgccggatc gagcacttct
     9001 aaaagcgcgc tggacgggtc cccgcggaaa tcctgcccga ttttgtctat ttcatccagc
     9061 ataaaaacgg gatttgaact ttggcaacgg cggatttctt gtattatttt tcccggtaat
     9121 gctccgatat aggtacgacg atgcccgcgt atttcagctt catctttgac accacccaga
     9181 ctaaaacgga caaattttct ccctatagcc cgcgctatcg agcgaccaag cgatgtttta
     9241 ccgacgcccg gaggacccaa aaaacatagg atagggcttt tgctgtcttt ccgtaatttt
     9301 ttgacggcaa gaaattccaa tatgcggttt ttaacatcat ctagatcgta atggtcttca
     9361 tccaaaatct ttttggcttt tttgagatca atcctgtccc gcgtagcatt ataccaggga
     9421 agctccagca gccagtccac gtacgttttg ctcacggtat actctgctgc actgggcgac
     9481 atttgcgaca gacggctgat ttccttcatg gccgcttcct tggcttcggc gggcattttc
     9541 tttttttcga cggccgcctt aagctcttcg acttcgaccg aaccgtcatc atcttcacct
     9601 aattcgtgac gaatggcctt aatttgttca cgcaaaaaat actcacgctg gtttttgctg
     9661 agttcggcgt tgacttccgt ttgtattttg ttgttgagct ccagcaattg tatctcgcgg
     9721 ctgatggcgg acattacctt cgtgagccgc tctttcacgc cgatagattc taatatttcc
     9781 tgcttttcgt tcaattcgat attgagactc gaagcaacca tatcggccag cttatcgggc
     9841 tcacgaatgt tgctgacgat aactttgagt tcgtcgggca gtataggcga gagcgtaacc
     9901 attttttgaa aactttccga cacgccgcgt ttcagggcat cggtttccaa atcatccgct
     9961 gatggcacat ggattggctc cacctgaccg ctcagataga ggccatccgg cgttgccgct
    10021 ttgaggcgaa tacgcgaaaa accctgaatg agcaaacgca cactgccatc gggaacctga
    10081 aacatcttaa gcacattgac cgccgtaccg ataccgtaga gatcttcaaa tgacgggttt
    10141 tctttgtcag gctgacgtaa agtaaagacg ccgatgattt tgtgggacga aagcgcttct
    10201 tgcacaagct gaacatggtt tttttccatt accacaagag gaaatactac aaaaggaaaa
    10261 atcacattat cccgtaacgg gagtatgggt agtttttccg gaatcgctat ggaatcggat
    10321 ttcatgtctg ttgatatatt tgcttcaggc atagatagtt attattcgat cactatgttt
    10381 gtaacacgcg atgagcgcga ggtatctttt tttaatcgta cgataagaaa accgttttcg
    10441 taaccggctt tgatggcttg atcttgtatg cgtgtattga ctttgatacg ccgttcaaaa
    10501 gggccgaaat ctatttccat tttatgataa tgtctttttt cgctgaattt catttcatga
    10561 cgatgaccgc tcaccgtcag aatgccggct tcgtatgtca atcgtatatc gtcctgcttg
    10621 acgttggcga tatccatcac tacgataaat tcatcatccg tctccaagac atcggtgggc
    10681 gggtgccaac gtttctcgga gtgcagtaaa atgggaaact tggtattaaa aaggttggaa
    10741 aaaataaaat ccatatcatc atctacgccg cgtaaacggt ggatggaata ttttttatcg
    10801 tcttccatat cgtccataaa aatacctttt ggatattgcg cggagggttt tacttatacg
    10861 aggacaaata taatcagtta tacactgtaa aagcaagcgc atttaagctt catcgtgcat
    10921 aaaaaattat catttgccta tcgtttatac catggctaca ttagcttgtt caccccaatg
    10981 aacggaaagt gaggttcgga tttgatcctt gctacacaat actctacgcg tttcttttta
    11041 ttaatatgct ttttttatgg catgcccact gtagcagcgt ggggacagtt gcgtttggag
    11101 cttggctttg accgcaaaaa taatcttttg tggactacgg atccgggtat agaaaaagcc
    11161 gcatactcta atcttgatat cgaaacgtgg ggcgatttca agctgcgcaa tgatctgccg
    11221 caaagtccgg gcggcgacgg ctatctttcg ctaaacggca tccagcaata ttttcaaatt
    11281 cccgaacgcg ccggtcttca ggtttcgggt aatatatctt tttcgatatc tatgtggctc
    11341 cgtgttcccg ccggcacaca aaacggcgat attatcagca gcgacaacgg tttcatcaat
    11401 ggctatcgtt tatttttgga agacggtatg atccgttttg aaattcggga cggtaccaaa
    11461 gagatttttt cgtccaatcg cttactcatg cctaatcagt ggcaacatat cggcgttgtt
    11521 tgcgacgggc tcggcgactc ggttactttt tttataaacg gagcatctgt tgcttcgcat
    11581 ccttttacgc gcgtagtaca ggtacagtcc ggaacacaaa cctatatagg cacgcgtgcc
    11641 gaatcgtcat ttcccaatta tttgtacggc gatatcgatg attttcgctt cttttcaggc
    11701 cacgacacga ttttcgaata catacgcact atgccggagc gcgatctaca gcgtctatca
    11761 ctcaagcgcc ccaaaacaac cacaccgtca tattttacgt tgtaccaaaa ttatcccaac
    11821 ccgtttaata tggccactcg gattgatttt gaaatgatac ggcccggaag cgctgaactc
    11881 cagatatttg atttgctggg caatatgatg aaaccgatct tcaccggtga actttcggcg
    11941 gggcgtcata cctatacttg ggatggcaca gataacaata acacaccggt atccagcggc
    12001 atttattttg cccgccttac cgttaacggt attgtacaaa ctaaaaaaat ggtgttgatc
    12061 aaataatcgc tacggaacga atttgtaacc gacaccgtgg acagaaataa tatgcgtcgg
    12121 gtctttggga tttacttcaa aatagttacg caaacgtacg ataaagttat ccaccgttcg
    12181 cgttgaaggt atcgcctcgt aaccccatac tttcatcaat atctcctcgc gcgtcacgac
    12241 ttgctcctga cgttcgatca gtagtttcat cagcatgcat tccttaggcg tcagttgtat
    12301 tttttttccc cgtgcgacag ctatgtagtt ttcaaaatca atttcatttt tatcaaaact
    12361 aaatttactt gttgatgatt cattgacggc attacggcgc ttaagtaaag ctttgacacg
    12421 caataaaaat tcgtccaact caaacggttt acctaaataa tcatcagcac cgagttttag
    12481 gccttcgata cgatcgcgcg catcaccttt ggcggtcaaa aaaagtatcg ccgtctccga
    12541 atccgttttt cggatttcgg cacaaacatc gtaaccgctc tttttgggca gcatgacatc
    12601 caaaacaatc aaatcgaact tacttttttt ccattgcact aacgcatcct ccccggtttg
    12661 agcccagata acattatact cagtctctaa attgaactgc aatgattccg caatatgggt
    12721 ttcgtcttcg accaatagga tttgcgattt attcgtcata tttaatatcc ttttattttt
    12781 taagcgttgg aatactaacc gtaaaagcag cgccgccttc cattgccgat tgacaatcaa
    12841 tagttcctcg gtgcgcttct acgatacgtt ttacgatata caatccaaga ccggtccctt
    12901 tagtagttcg cgtcatttca tcgccagccc tataaaaacg ttcaaatact ttcgtacgat
    12961 cctcgggcgc tacgcccggg ccattatcgg ttacggttaa taccgcgcgg tcgcctatca
    13021 tagacagtaa gacattaacc cgtttaggtg cttgtgaata ttttacggca ttttcgataa
    13081 gattattgac tacggaaatc atggcatgcg catcggcgtg aatgtgaata tcggacgcaa
    13141 tgttacctga aaacgaaatc acgctttccg tatcggatgt tttccattta tctaaaacgg
    13201 atttaaccag ttgcgacata tcaagcgaac ccatctgata atcataatca ttacgttcaa
    13261 tacgggcggc atccaagaca ttttccacta agcgttgcaa ccgctccaca tcggcaaggg
    13321 agtgttgaac aaattgttcc cggcgttctt ccgtaatctg ccgccgttgc agcgtttgca
    13381 aatacagttt gatcgatgaa agcggcgaac gtaattcatg cgtcatactc agaatgaaat
    13441 ttttttggtg ctcctgtaat tgtaactcgc gccggaatgc ggcatataaa aaataaatcg
    13501 caatcatcac catcaaggca aaaaaacttc cttccgataa aaacatgacg gtccgtcggt
    13561 ccatttgttc ctgcaaatta tcttcggctt tgtatttagg aatcaccgtt aagggcaatt
    13621 ccaaataggt tgccggcgca gagctgatcg taggcttttt ttgcagtaca aatccgctat
    13681 ccgaagcggc taatttttgt tccagaaaag gccaattaat cggaaaaaaa tagatcagat
    13741 cctgataacg aagaaatatc gtatcgggcg ttaccacaaa atgtgaacgc ggccagtcat
    13801 tttcttgggc ttggcgaagt atgtgcaagg ggtggttcaa cgatccttgt atcagatcgc
    13861 ggtattccaa tgtcagaaaa cgacgcaact ccaatgcttt actgcttatc atttttttat
    13921 gcagattcat cacggtacga ttttgctgca cctgaaaata catccaccaa aaaatttggg
    13981 ccaggcacag aagtacaata acgataaaaa taactatggt tttcttttgt agtttcacgg
    14041 cggtgtttac aaggtttgtt tatctttaag cagggcttcg taagccgctt ttatttcttt
    14101 aaaacgttca tcaccgacaa taccggtatc atcggcggcg tttgcatccg gatggtagat
    14161 catgctcagt ttgcgatata ccgctttgat ttgatcaatg ctcgcatcct ctttgatgcc
    14221 gagtatatca tagtacgagc ggtgcgcctt gctggccgat gaggggtaaa ctccacccaa
    14281 tcgcatggcc tcttcgacaa atgatttgat ctgttcaacc tgcggcgtta cctcagcgac
    14341 ggacagttga ccgttttcaa aaccggattt gagcacctga agttccgaac gaaaatccat
    14401 tacggccata ataatatcgg cactggagtg accggcaagc gcttcgatga tacaggtttc
    14461 cgtttcttgt atcagcgtta tcaccgctgt ttcacgttgt gtttgcaggc gctggcgtaa
    14521 atcacgagta tttcgctgaa tatcatcagc gatttctttg gccatgacat aatgcttttg
    14581 aactaaagcg tcttgagcac gttctaaata aaagcgatac tcggaaattt cttttggaaa
    14641 tgaaacgccg tcttcagatt cagaaaaatt tttcaaatcc agttcagcat tggacacttc
    14701 aagcaacgct tctttgcgca actgcttata acgctccgct gaatccttgg cgcgaatcgc
    14761 caggcgcacc acttcggcgg ataatgactc acataaacca tactgcattt cagcaaaagc
    14821 cttctgcaaa gcggagaatt tgtccaaggc ggatttaaaa tcttccgaag cgatcatttc
    14881 ggcttcagcc gtacgcgcta atacgatatt ttcatcggca tcatcaaccc accattgtgc
    14941 ctgtcggcgc aactcttgac gacgtgatcg cttacgcata taaaaaataa ggacggccaa
    15001 aaccaaacac ccggaaacag ccacaaaagt gaaccgaata ttgatccagc gcgactcttc
    15061 ttcgatttct tgcttgagtt ccaatgcgtc ggcaaattcc ggatcaaatc ctaatgccat
    15121 atcgagaaat ccccgcgcaa tgcgataact gtctttttca tactccgtcc gcgcattttt
    15181 ggtgtacata tcggctagtt gactggcggc ccgtttatga agagatttgc cttccgtatc
    15241 ggttttatca atttctaata tctctttcgc attttggaaa gcctgatacc aattttcttt
    15301 agccagatct ttggcggcct gtgttttgag gtaatcaaga cgcgtttttt tatcgctgcg
    15361 aacataatca taatgcgaac cgatggataa tcggtcttcg ccggccatat atattttgga
    15421 tgtcgtatca attctaaagt cggaaatcgc cgcattcaaa aacatcggat catggctcac
    15481 atcggtgtga tgggcttcga tgccttcata taatacgcca ttattccaca cgttattatt
    15541 ggataacaaa gcaagtgtac cttgtttttg cccgataccg atatgattcc acactatatt
    15601 attattgatc aaggccaggc ccgatgcatt tcttatcaaa acaccataac ccgtttcctt
    15661 ttcggcatac cggctcgtat catttttatg aaagactatg gtgttattcc gtattaacgg
    15721 attggactgt ccatcaatag tgattccatt ttcaccattg cgatatatgg tattgccgat
    15781 gactttcccg acagatccga gatttatcaa taaaccgtct tttttgttat cacggataat
    15841 attgcgctcg atcagcggcg atgatttatc gctgacgcta attccggaag cttcgttttg
    15901 ataaataaag gactgtgtga tcgtaccttg acttcggtcc gatacactga taccgtaacc
    15961 ggtattatcg ctgatactgc actcacgtaa tgtgactgta ctattgcttt tcatttcaac
    16021 gcccgttgcg ccgcgtctga aagctacacg atcggctgaa aaattggaat accgcaccag
    16081 caaaacggga aatgccgtag gttgatcaaa ttcaaaactc aaatcacgaa gccgaagtga
    16141 gctgatattg taggcttgaa tgacgggagc cgttggtgat ccgatcacga gtgttcgtcc
    16201 cgcaggatcg ccttcaaggg atataaaatt cctaaggatt agattctctc tatatatccc
    16261 ggctttgacg cgtataatcg aattcagttc cgatttatca accgcttctt gtatcgtgcg
    16321 gtagcgcccg gtgccatccg aagcaacagt atagggcctc tgtaccaacg gtctcctcgt
    16381 agaacgggaa acttgaccgt tcacctgcgt ccaaaaaaga acgaccgata cacaggtaaa
    16441 gcgcattatt tttgatatca aaggcatatg gtttgtaatg taaatcaaaa ttttctgaca
    16501 ttcaccaaaa atggtaaacc ctcattaaac gataacttaa tgagggttca gtgaaaaaat
    16561 gtatactgtt tgattactgt cgggagtgaa tcggtccgtt ttccaaatta agtacacctc
    16621 ttgggcacac cgctgaacat attccgcacc cgacgcacga agctcgcacg atattctgtc
    16681 cacgctgagc gtacgaacga acatcaattc ccatttcaca atacgtcgaa caattaccgc
    16741 aactgataca ttgcccgcca ttggtggtga tacgaaaacg cgaaaaatat ctttgcaata
    16801 ttccgagata ggcggccata gggcaaccaa aacggcacca tacacggctg cccaacaaag
    16861 gataaaaacc gacgccgatc acaccggaaa ataatgcacc gataacaaat ccgtacacat
    16921 tcgaaaaaag tgatgatacg tttccgagta attggccatt ggataaacta ttaatccaaa
    16981 caaccgctgt tgtgatcact acaaaaatta aaacaccata aaccgaatac cgttcgatct
    17041 tccacgagcg taaggattta tcggataaat gtctgtaagg atcgcccaga gtttccgcta
    17101 aaccgccaca gccgcaaacc catgaacaat accaccgttt accgaaaaaa tatgtaagta
    17161 tgggcgttgc aataaaactc atcacggcgc cccaaaacac cataaatgta ccaagccctc
    17221 cgggttggga tattaaccaa ttgatgtcgt tgggaaacaa atagctgtat ttaagaggcc
    17281 agaagtatga aaaataaaac tcgggttgat tgaatttttg gagcaacccg ggtaagagaa
    17341 atgcaaaacc aagctgaaaa aatattactg aaaccgttcg gatcagttgg taacggctat
    17401 gtctgttttt ggccatcata cgcaaaccca ttaccatcac tgccagcgta taatagaatc
    17461 cgtaaagaaa ccattgatca gcgggtttat ttctgagaat gccgctcaac caatctgtcg
    17521 tgtgtatcag gtattctagt cgctcaggaa accagtaaag taccacataa aatccggtaa
    17581 aaaatacggc ggcaatccat ccccaaaccc cgcgattgac gaaggaatta aaaaaaattc
    17641 gatcgtgttt tataccgggt tttctgtcat accacatggg catgatgtag gctaatgctc
    17701 ctatggttat aagcccgctg gaaagtaaaa acatcccgat tggatattgt tcggatatcg
    17761 gacttagagc aacgccaagc atcaaaaaac ctataacaac taaagcgaga gatatctttt
    17821 ggtgcaaact tagtgtaaca aggggtatct gaatcgggac gggttgattc ataggggatg
    17881 atctatgtaa gatttaataa tttatcgtac aatgaagtgg aggccgcgct ggacctgcaa
    17941 aagaagattt tgctcaggat actttcggtt gtattcagca atgatatgcg gttcgtactg
    18001 cataaaaaat tcgggatcgc aattagcttc gcccaaattt tgaagaacat agtcgatcgt
    18061 tcggctttct tttatccaac gttgacatac attttgtctg tagcgtatcc ccatcagatt
    18121 aaatccgatt acaatccggt tatcttttcg aaaaacaatt cggataccat gctgtccgtc
    18181 aggatgttcc caccagagat gctcttcgcc gtcacggggt acgtttggca ctacaccata
    18241 tgtctgatat tcgatgtcaa aaaatttagc cgagttaaac caaatccctc gatcataagc
    18301 ttggcgttca cccgtcaaaa cacgcgccaa cgcttcacct tgcatgcgac ccgtgtacca
    18361 aagttgttca attttgggat gcgctggctt gggttctatg aactccgcac aatcaccggc
    18421 ggcataaata tcagcaatcg gcgtttccaa aaattgattg atcagtacac cgcgattcag
    18481 cggaattccg gaagaacgta aaaattcaat attaggtttt acaccgaccg tcaaaccgac
    18541 aaactcacac ggtataactt ctccggactt agtcacaatc gcatccacgc gacctttatc
    18601 attaccgcga atctcctgaa gttcggtatt caatcgcaaa tctacatgat gcgcacggat
    18661 gtggcgatta atcatatccg attcttccgc cggcaaaata ttatcccagt aagatttttc
    18721 tcgtacaagg aaagtcacgt gaatgccgcg agagatcagc atttcggcca tctcgatacc
    18781 gatcaatcca cctcctacga tcacagcacg ctttaccgac ttggtgtttc gctccatcag
    18841 ttcaagatct tgcaaagaaa atagtccctg cacaccttga agattttgac ccggccagcc
    18901 gaatgtttga taaacagatc cggtggcaat aactaattta tcgtacttca tggcctcgcc
    18961 gtttttcagg taaatctgtt tttcggcagg gacgatgcgt tcgactaatc cacgcctgag
    19021 ttggatttca ttttttttcc aaaaatcatc ttcatagggc ttagtgtgct cgtacttcat
    19081 gtgccccata tagatataca tcagtgccgt acgggaaaaa aaataatcac tttcaccgga
    19141 gattatagtg atcggttgat cgctgcgttt acggacatgg cgggctacgg tagtaccggt
    19201 tataccatta ccgataataa taatttggga cataatggat ttattaaaac gggttatgcg
    19261 ttgcccgtat tgcgcttttt tcgtttatgg gcaatatgtt ttcgttcact agggctgaca
    19321 tagtttgatt tttcgataca tgtttgaata atccgcgcct tgcgcacttc tgtttcaaaa
    19381 cgacggatag ctttctcaat gggttcgttc ttccgaacaa tcgttgataa catggtacac
    19441 ctcacaggtt agatagaagg atataccgca tatacgcaaa cacccggcca aagtttccgg
    19501 ttgcttcatg taacttacac tgtgagtttt tattccctgt tatttgataa acaaaatctt
    19561 tcgagagtag gttttcccgt tagctacgac cgttacgaaa tatataccgc tggctacttt
    19621 attaccgtgc gtattttttc cattccattc acgcttatat atccctggcg aaagttggcc
    19681 gctaactaat ctatgcacca aaccaccccg cacatcgtat atatacgctt tcacttccat
    19741 cgttttattt attttcataa cgatgctggt tcgttcatca aatgggttgg ggctattgta
    19801 aagtagttgg agcgggcttt cttcgtacac tgaaacattg atttcaaatt tagctgtatc
    19861 ggcgccgtct atgttgtagg cacgcacttc tacgacatga gtgcctatat ccgtcaccac
    19921 cggtttccac tgcactatac cggaaatcga atcgcacttc agtgatgacg gccctgataa
    19981 tatttgccaa cgcggcgcag gattagcgtt ggcgataaga cgatacgtcc atgatgaatc
    20041 cgatcgaatt ttttgtttgt ttggaatttt tatgattggt ttttgtgtac cggctctata
    20101 gaattcgata cctcctcgca aattaccgca cactatatct ttatcgccgt cattatccca
    20161 atctaaagca aacggcacag cttgacctgt gactttaata gtcgaaaact ttcctgcgat
    20221 ctcctgaaat gagtttgcat tctgccgctc ataataacgt agcgtcccat ttttgtagcc
    20281 tacgatcaaa tcacgatccg aatcaccatc ccaatccaat aatatgggga cggcatcgga
    20341 aagaccgttt gcagcgatta atgtaacggt tgccgtagaa aaatcaggaa ttgacagagt
    20401 accgttatta gatatgagtt gaacttctcc gctctcttct ccaataatca aatccagatc
    20461 gccgtcgccc tcaaaatctg ataaaaatgg cgagctatta ttgcctatgt ccaaatttgc
    20521 ataaaattga ttctctaatg tgaattccgg actcgttaaa ctgccggtat tgcggtaaaa
    20581 cattatttta ccgtcgaatt ttccgacaaa taaatccaaa tcgccgtcat tatctatatc
    20641 tcctaaagca ggtgccgtaa taaattcatc ttgtggtatt gtgacaaacc atgaagaatt
    20701 gctttggaaa accccgttga ctgtctgacg ataaaaatgg atcgccccgc tatagctacc
    20761 aaggataata tccaaccggc cgtcgttatc cagatcagcc attatggggt gggcattacg
    20821 accgacatca atgttttgta atagattctt tgtgattaac tgaaattggg ccgcttgcgg
    20881 tgttcctatg tttctataat accacaaatt atcccggtct tcttcacggt ataatacgct
    20941 taccaaaaga tcaccgtcgc cgtcaccatc ggcgtctaca aatgacggca tgttgtatcc
    21001 taatgttttt aactgccgcg atgggtaagt agatgtagcg gtatccggaa taaaaggatg
    21061 cgccgaatcg ccaaggtttg gtaaaaaata caaacccgta ctgaagaaat cgccccaaaa
    21121 taaatccgga tcccgatcat catctatatc aaaaaaacaa acagcacttg caccatgtct
    21181 ttgctcttcg ctttgcttag caggggtgat gatcgcaata tcttgaaacg catcagtaat
    21241 aaattttaat aaaaaactgt gttcgttccc cacattttca taatacgaca gtgttccgac
    21301 acttctacca gaaaaaaaat cataatcacc atcagagtca atgtctttaa aaacgggtac
    21361 agtctggtct tccgttaaga cgggtgcatt gagggtatcc aacacggttt ccgtttgcaa
    21421 cacaaaatgt gcatttaccg cgctcccttc attcttatag taacccatac gtgcaccgga
    21481 aaccccacaa aataaatcca aatccccatc cgcatcggca tcaataaaac gtacccattg
    21541 attcacttct aaactggcaa aattatccgt tatccatagc caccgcaaag gcccttccac
    21601 gccgctattc atgtaatgcg ttatacgtcc atcggtttgt acgatgaaca aatccggatc
    21661 accatccgaa tcaatatctg caaactccgt cttaggtaca tacatcccgc cgtgcgatgc
    21721 gtatgggatc atgccatcct gatcgtagaa tgtcaacgct tcggtatctt tgaaataatt
    21781 ttgcgagtaa agtacgcccg ccgcacatag aagaaataga gaatatctaa cgatcataat
    21841 gagataataa gctacttaaa gaaacggtgt ctgatctcac ggaaacaagc taacagcaat
    21901 ataggtaaaa atatcacggc actaataata tgaatactct ccggaatcac atcgtgcatc
    21961 gtacctatag atacttgcgg ctttatatca aagtaggaat atacactgtc agttaatgac
    22021 cccattgcca atgcaatcat agcaatagaa aacaaataaa taattagtga cctttgccca
    22081 agtttagaac gcactaacgc aatcgttgaa atgttcgttg caggccctgc taacaaaaaa
    22141 atcagtgcgg ctcctggagt aatgcctttc atgatcaaag cagcggctat gggtgttgac
    22201 gcactggcac acacatatat tggaattccc accgcaagca ttatcatcat attcaccagc
    22261 gggctatgca tcacatcaat aaaaaagttt tctggtatga ttgccgataa aatgccagag
    22321 gcaatcagcc caagaagtaa ataataagct acatcaccca gtaaattcgt aaaggcatat
    22381 acaattcctg attttaaacg atcaaatcgc gactctacag ttttatcagc ttcgttatta
    22441 caatttgttg aacaattaca cgaagtctgc ggggcgtcac tttgctgtac agtgtcgctc
    22501 tgcttttgtg aaccaaaaat attttcaagt gaaccgctga ctatggcagt aatgaatgcc
    22561 gcaatcggtc taataatcgt catgatagga tccaaaaagg catacgatac tgcgatcgag
    22621 tccacccctg tctccggagt ggacactaaa aagctcattg tagcaccgtt acttgcgccg
    22681 ttttttttta gcgatattcc gaccggaacc acactgcatg aacataacgg caa
//
LOCUS       DAOQGF010000047        22536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1057_length_22536_cov_7.404075, whole genome shotgun sequence.
ACCESSION   DAOQGF010000047 DAOQGF010000000
VERSION     DAOQGF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1057_length_22536_cov_7.404075"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK937_08170"
     CDS             complement(<1..277)
                     /locus_tag="PK937_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32584.1"
                     /translation="MNKRVSPPWALLLDVTLCIFLLFFGAPIYGQTLDSNVSATTVLP
                     QVSRDSLRTFLLGKPHGNEKKSVPRMTFTGDTLFRSPWGAVGRSFLVN"
     gene            complement(270..1211)
                     /locus_tag="PK937_08175"
     CDS             complement(270..1211)
                     /locus_tag="PK937_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNH32585.1"
                     /translation="MKIGFAYAPIFLEHRMYAGHHPECPERLGAITQAIAEAKLNDTL
                     ENLHIKPADESLLVEVHSEVHIDRIKEEASRAPAMIDGDTYVAHASYEAARMAAGAVV
                     VAVDAVCSGQLTRAFCAVRPPGHHAEKEKAMGFCLFNNVAVGATYARRAYGLQRIAIV
                     DWDVHHGNGTQAIFYEDPTVLFISTHESPLYPGTGQHTEIGNGAGKGYTLNIPMHAGD
                     GDMEMETVFDKQIIPALDQFKPELLFISAGFDAHARDPLANMTVTSEGFGKLTRLVVQ
                     AAEKHCQGRVISVLEGGYHLEALGASVVSHIRQLAHE"
     gene            complement(1208..1558)
                     /locus_tag="PK937_08180"
     CDS             complement(1208..1558)
                     /locus_tag="PK937_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH32586.1"
                     /translation="MTFKEEKKGDVTIVYVHGKLMGGMETSAVHDRVHVLAESGVKKL
                     VLDLSQVKWMNSSGLGVLMSSMTTMKLHGGDLRLSAVIEKVESLLMITQLLKIFKTYK
                     TVEEAVQSFSSQDV"
     gene            complement(1568..2254)
                     /locus_tag="PK937_08185"
     CDS             complement(1568..2254)
                     /locus_tag="PK937_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32587.1"
                     /translation="MQRIVLILIAGIFTTGFQSGSTLVGYHAKIRTTVYEDLSVARAI
                     ELSMDADLKEAVKYKKDFFRKNGYELIEKNENDRLTIVAQKVFPTASHRIEKYEDDLS
                     AITVEKEGLRFSYREDFYTKMFKEEIERSDLRNDLPVAKMLMADTKFEFYITLPGKID
                     SLNSGEFDKATAHWTYDVDELMEHKSMTMRAHSTADRGDIQIWMVFLIGVLLLLMAYL
                     LRIKLRMRAS"
     gene            complement(2382..3134)
                     /locus_tag="PK937_08190"
     CDS             complement(2382..3134)
                     /locus_tag="PK937_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH32588.1"
                     /translation="MSRLRIIIIDDAVKARETLRLMISENMTDIEIVGEAGTLPDGVK
                     LIHKTNPDVVLLDIEMPGHSGLEILDFFSPERIHFKIIFVTAYAEHAVRAFELSAVDY
                     ILKPVSPNRLKQALDKVTLGQINAEQYQVLKEQTAGTWNKIALKTGDGIVFIPTDQII
                     YLKAEGAYTKFILMKQETILVSKNLAEYEKLQSAGPFVRAGRSIILNLSRIKKIGKAS
                     DGSVTMDNGDTLAIGDETYKQLVEAIKTIKLG"
     gene            complement(3131..5020)
                     /locus_tag="PK937_08195"
     CDS             complement(3131..5020)
                     /locus_tag="PK937_08195"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH32589.1"
                     /translation="MRIINSLFVITALIRCDLTAQEVDADSLLRKLRVYPIRDSARVE
                     ILTDICAALTFKEPDSVLIYAEEALIISKEIGYEYGIATAINCRGMAYYQKGDLTKAM
                     ENYLQASDYVKQHRIRNPRLDYADFVILNNIAIVYNNKQLVDEALAIHFQQIELLPLI
                     SANPIHYAALYTNIGGCYGNKKMFAEGIRWFEKVDSIAKVYNIPYGTSIAMLNIAANY
                     NNMKLYSKAITSFRQSLTIAQALRIADTESACHNGLGVAYSETGRWAEAVLSFEAGAE
                     VAKRSGLLSNAMTAYQGLARAAVQTGDFRKAYEAQMQYQQINDSLLSIEKTKSLQELI
                     TRYETEKKEITIREQQERLKVQSLESDRKNILIVGIILLLAVVGGASFFYIQTLQAHK
                     KNEILSMQMIAAEKSKAMEVAIAQSELKGLKSQMNPHFIYNVLNSIQSFVYANQKNEA
                     SENLSKFSDLMRKTLENSGRQWITLHEESEYLSRYLELEALCADHAFQYEIVVDPGLD
                     PYTVEVPAMVIQPFAENAIKHGLLHKSGSKKLSIRFEKNLSGNDLRVVIEDNGIGRAA
                     SAEINARNGKKPRSFATQATAERIALLSQLQSRSIVCQFTDEPSEHGAEGTRVDIVFP
                     LEDRA"
     gene            complement(5032..6162)
                     /locus_tag="PK937_08200"
     CDS             complement(5032..6162)
                     /locus_tag="PK937_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNH32590.1"
                     /translation="MKLLSKYLFIGFVLLSYGLPSNVTVAQEKARIKAIWVVRDILKS
                     KIEIDRMLSFCDQNGITDVFLQVRGRGDAYYNSDFVVKAEGLDPAFDPLQYVIEKNRL
                     KKIKIHLWLNVFYLWSSEKNPVHPEHLFYTQPDWAAVNAQGQSMSVEGTKKLIAKRLE
                     GVFISPAIQEFQDYFSAVVKELVSKYPVDGIHFDYIRYPEADYDYSTSMRSRFILEYH
                     WDPLAEALNAWQDSIWTRFRKRIIEDYVACIYTQIKKADSSIAVSAAVWASQDDAQNR
                     VLQDWPEWIKSNCLDFAVPMNYATDNGEFEKRIQDAVIKLGASDTQKIIMGVSLYNQP
                     VKSAEEKLRICRKYGFAGVSFFSYETLRTQKEYKTLVKNFNH"
     gene            complement(6292..6618)
                     /locus_tag="PK937_08205"
     CDS             complement(6292..6618)
                     /locus_tag="PK937_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32591.1"
                     /translation="MPRTKKEAADEKETKAPKKKAAAPRKKKEEVKTEAKATVSKKTE
                     AASEVLLPKIQCFAPGKKTICAHLMTSGHAHQCAAKRLPNGKLSTVRSGKKRRCEFYQ
                     ETTYNG"
     gene            complement(6638..7204)
                     /gene="rplQ"
                     /locus_tag="PK937_08210"
     CDS             complement(6638..7204)
                     /gene="rplQ"
                     /locus_tag="PK937_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNH32592.1"
                     /translation="MRHGKKQIKLSKTASHRQALIKSLCNELFRHKRIVTTISKAKAA
                     RMTAERLIMFAKKGDLAARRILISRLGNARLVTAHKHVTDKKELSKTVIHELINEIAP
                     KMKELDAQRKAKNASYTGGGYTRVLRLGLRKGDATEMAVLELVGYETASIDKQNKAIE
                     AKEAKAQRAKSLAERIKAKKEEAQKESK"
     gene            complement(7229..8221)
                     /locus_tag="PK937_08215"
     CDS             complement(7229..8221)
                     /locus_tag="PK937_08215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNH32593.1"
                     /translation="MQNWAGLQMPERIELDESSYTTTYGKFIIQPLERGFGVTIGNSL
                     RRVLLSSLPGAAITAIKIEGVLHELSTIPGVAEDVAEMVLNLKGVRFKLHNKKPDKVS
                     IRLKGPHKFTAADIQKASNGIDFEVLNPDHVIATLSKEANVEMEIRIGRGKGWVTAEE
                     NKFPDMPIGTVPIDSIFNPIKNVRFTIDSTRVGQHTDYEKLTLEVTTDGSITPDDALT
                     IAARLFRDHIQLFINFEMEDENADGEVEDQEAARIRQLLKMSVDELELSVRSHNCLRA
                     AGIKTIGDLVSKNESEMLKYRNFGRKSLTELTKILEERNMSFGMDVSKYYNGEE"
     gene            complement(8285..8908)
                     /gene="rpsD"
                     /locus_tag="PK937_08220"
     CDS             complement(8285..8908)
                     /gene="rpsD"
                     /locus_tag="PK937_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNH32594.1"
                     /translation="MARYTDAVCKLCRREGEKLFLKGTKCLTDKCPIERRNYPPGEHG
                     DKRSKPSGYSIQLREKQKLRRIYGVLERQFRRYFEKAERAKGVTGTALLQILETRLDN
                     MVYRFGFASSRSQARQLVLHRHVEVNGKLVTAPSYQVKPGDDVSIREGSKKMALIHGS
                     LKRQKETSLNWIDVDKASLKGKFLNMPEREQIPVDVKEQLIVELYSK"
     gene            complement(8944..9315)
                     /gene="rpsK"
                     /locus_tag="PK937_08225"
     CDS             complement(8944..9315)
                     /gene="rpsK"
                     /locus_tag="PK937_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNH32595.1"
                     /translation="MPRKKKKLIESTGVAVIRATFNNTIVTLTNSHGEVISWCTSGKM
                     GFRGSKKSTPFAAQVAAETAAKTAIDMGLKKVEVRVKGPGAGRESAIRSLQAAGLEIT
                     AIRDLTPIPHNGCRPPKKRRV"
     gene            complement(9382..9765)
                     /gene="rpsM"
                     /locus_tag="PK937_08230"
     CDS             complement(9382..9765)
                     /gene="rpsM"
                     /locus_tag="PK937_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNH32596.1"
                     /translation="MARIAGIELPKDKRAFVALTYIYGIGRTTAKDILKKAGVDEMKK
                     IKDLTPDEENKIRTIINDNIKVEGALRSENALNIKRLMDIGSYRGLRHRRGLPARGQR
                     TRTNSRTRKGKRKTIGGLKKVEAKK"
     gene            complement(9791..9904)
                     /gene="rpmJ"
                     /locus_tag="PK937_08235"
     CDS             complement(9791..9904)
                     /gene="rpmJ"
                     /locus_tag="PK937_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNH32597.1"
                     /translation="MKVRSSVRKICVKCKIIRRKGVVRVICDNKKHKQRQG"
     gene            complement(9917..10135)
                     /gene="infA"
                     /locus_tag="PK937_08240"
     CDS             complement(9917..10135)
                     /gene="infA"
                     /locus_tag="PK937_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNH32598.1"
                     /translation="MAKEGLIKVDGTILETLPNATFIVKLENGHQVHAHISGKMRMNF
                     IRILPGDKVTIELSPYDLSRGRITYRYK"
     gene            complement(10230..10988)
                     /gene="map"
                     /locus_tag="PK937_08245"
     CDS             complement(10230..10988)
                     /gene="map"
                     /locus_tag="PK937_08245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNH32599.1"
                     /translation="MIRLKSGAEIDKIRESCRIIVEAMMVAAKMVKPGLRLMDLDAAI
                     ESAIKSRGGKPAFKGYHGFPNNACLSTDDIVVHGIAKPGDILQEGQILGVDIGVLKNG
                     FYGDSAVTLPVGEISEEKKKLLQVTRESLYLGIQKAVPGNRVHDISAAVQTYVEHHGF
                     SVVRELVGHGIGRELHEDPQVPNFGKAGTGARLKEGMVFCVEPMVNAGTYEVYTSKDN
                     WTIHTKDGKPSAHFEHTVVVTSNGPEILTQSDLF"
     gene            complement(10994..12325)
                     /gene="secY"
                     /locus_tag="PK937_08250"
     CDS             complement(10994..12325)
                     /gene="secY"
                     /locus_tag="PK937_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNH32600.1"
                     /translation="MLQTFANVFKIPELRKRILFTLGVLLIERIAAHIPAPGIYAEAL
                     GQYFRQAENTLLSFYDLFAGGGLSQATIAALGIMPYISTSIIIQLLGTTVPYLQRLQR
                     EGEEGRKKINQLTRYGTVALAAVQAIGVATWLCSLSVVIGSQEVKVVPDQGFTFYLMT
                     MVTMTTCTMFIMWLGEQITERGIGNGISLIIAIGILARFPTAIIDEITQISTGNRNII
                     LELILVTILLGIVAAIIFITQGIRKIPVQYARRVIGRKVYGGQSTHIPMRINIAGVMP
                     IIFAQSIMFVPNTVATFFPGDFSEWITAHFSIDSITYNLFFLILIVIFTYFYTAIAMN
                     PVEVADNLKKQGAFIPGVRPGKKTSDFIDNILTRVTLPSAIFLGVIAILPFFVRHATD
                     VSYSFASFFGGTSLLIVVGVGLDTIQQIESHLVMRHYDGFLKSGRIRGRRG"
     gene            complement(12361..12846)
                     /gene="rplO"
                     /locus_tag="PK937_08255"
     CDS             complement(12361..12846)
                     /gene="rplO"
                     /locus_tag="PK937_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNH32601.1"
                     /translation="MNLSNLTPAKGSRRKMKRIGRGEGSGHGGTSTRGNKGQKSRSGL
                     NRLAGFEGGQMPLQRRLPKYGFTNIFRVEFQTVNLADLAELDNKRVDGATLQAAGLIK
                     KKSAPVKLLGTGEVTKAFEVEVHAISATAKEKIEKAGGKVTVLGIKKTVLEKRKKKAS
                     K"
     gene            complement(12944..13120)
                     /gene="rpmD"
                     /locus_tag="PK937_08260"
                     /pseudo
     CDS             complement(12944..13120)
                     /gene="rpmD"
                     /locus_tag="PK937_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
     gene            complement(13144..13662)
                     /gene="rpsE"
                     /locus_tag="PK937_08265"
     CDS             complement(13144..13662)
                     /gene="rpsE"
                     /locus_tag="PK937_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNH32602.1"
                     /translation="MKKKEAQDNPADVELKEERVVHINRVAKVVKGGRRFSFNAIVVV
                     GDGKGKVGIGLGKANEVPDAIAKGKEDAKKRMIFVPLHGGTLPHEVSAKFGAGKVLLK
                     PATKGTGIIAGAVIRAVVESVGITDILTKCKGSSNPHNVVKATMKALSEVTNAREVGA
                     VRGMTVMDVFTK"
     gene            complement(13712..14074)
                     /gene="rplR"
                     /locus_tag="PK937_08270"
     CDS             complement(13712..14074)
                     /gene="rplR"
                     /locus_tag="PK937_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNH32603.1"
                     /translation="MADKNIVKAVKRQRVKYRIRKRIIGTAEKPRLSVYRSLNHIYAQ
                     LINDEAGHTIAQVSSKTKGIQKESGKVSLSKAVGMSIAKLAKEKGIEVAVFDRNGYKY
                     HGRIKAVAEGAREAGLKL"
     gene            complement(14105..14647)
                     /gene="rplF"
                     /locus_tag="PK937_08275"
     CDS             complement(14105..14647)
                     /gene="rplF"
                     /locus_tag="PK937_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNH32604.1"
                     /translation="MSRIGKKPVAIPDKVTVTVKGSVVTVKGPKGELTKQFHPEAMVE
                     VKDKEIIVSRKNNTNFQRALHGSVRAIIAGMVTGVSDGYERKLDIIGVGFKAEAKGKV
                     IQFNLGFSHPILFAPPTGVEIAIPTPTSVSIKGINKELVGQVAQKIRSFKMPEPYKGK
                     GIKYSDETIRRKAGKAATKK"
     gene            complement(14682..15074)
                     /gene="rpsH"
                     /locus_tag="PK937_08280"
     CDS             complement(14682..15074)
                     /gene="rpsH"
                     /locus_tag="PK937_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNH32605.1"
                     /translation="MTDPIADYLTAIRNAIQAKKKFVDIKASNLKRDITQILLEQGYI
                     KDYANYDDGVQGIIRIFLKYDEKNRNAINQIDRASTPGLRKYAGVDKLPRVKSNLGIA
                     IMSTSKGVMTNKRAKTENVGGEVLCYVS"
     gene            complement(15129..15314)
                     /locus_tag="PK937_08285"
     CDS             complement(15129..15314)
                     /locus_tag="PK937_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNH32606.1"
                     /translation="MATKAWVAKTKKTPKFSTRTRNRCRRCGRPRAYMRKFGICRICF
                     RNLSLNGEIPGIRKASW"
     gene            complement(15349..15990)
                     /gene="rplE"
                     /locus_tag="PK937_08290"
     CDS             complement(15349..15990)
                     /gene="rplE"
                     /locus_tag="PK937_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNH32607.1"
                     /translation="MAKEAKEKKGKDKAASTGVEIPSGKRAAVGKDYKARLGEEYRKR
                     VVPALMKKFSYKNIMQVPKIEKVVINMGVGMATQEPKLLDAAVNDLLIITGQKPTIRK
                     AKKAISNFKLREGVGIACFVTLRRAAMYEFLDRLINIAVPRIRDFKGVPDKSFDGRGN
                     YTLGVKEQIIFPEIDVDKMDRVRGMDITIVTSAKTDEEAYELLKEFGMPFKKK"
     gene            complement(16024..16329)
                     /gene="rplX"
                     /locus_tag="PK937_08295"
     CDS             complement(16024..16329)
                     /gene="rplX"
                     /locus_tag="PK937_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNH32608.1"
                     /translation="MHIKKNDLVAVISGVHKGKQGKVLKVLTDTGKVIVEGINLRKKH
                     IKPNPKNPQGGIQEKENAIPASKVMLVHGGKPTRTGAKKLEDGSKVRIAKKSGEVIN"
     gene            complement(16368..16736)
                     /gene="rplN"
                     /locus_tag="PK937_08300"
     CDS             complement(16368..16736)
                     /gene="rplN"
                     /locus_tag="PK937_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNH32609.1"
                     /translation="MIQEFTRLTVADNSGAKKVMCIRVLGGTSKRYAFLGDEIIVSVK
                     DAIPGGTVKKGAVARAVVVRVRKEMRRKDGSYIRFDENAAVLINKDKEPTGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            complement(16769..17050)
                     /gene="rpsQ"
                     /locus_tag="PK937_08305"
     CDS             complement(16769..17050)
                     /gene="rpsQ"
                     /locus_tag="PK937_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNH32610.1"
                     /translation="MSEQAENKTTERNRRKVRIGTVVSDKMSKSIVVAIERKVLHKTY
                     KKYIKKTAKLMAHDEQEQAGIGDIVKVMETRPLSKNKRWRLVEVVKKAD"
     gene            complement(17097..17486)
                     /gene="rpmC"
                     /locus_tag="PK937_08310"
     CDS             complement(17097..17486)
                     /gene="rpmC"
                     /locus_tag="PK937_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNH32611.1"
                     /translation="MKAHEVRGLPAEELKGMINEAETNLVKMRFTNAVGQLEKKTDLR
                     STKKEIAIVKTVMREETLKTELAEAAKILEAVVSKHQIKGIPTLKGEKLNTDKAALRK
                     AVRLLESNPKRSLFVKEYKALKEIAHG"
     gene            complement(17511..17930)
                     /gene="rplP"
                     /locus_tag="PK937_08315"
     CDS             complement(17511..17930)
                     /gene="rplP"
                     /locus_tag="PK937_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNH32612.1"
                     /translation="MLQPKKVKFRRHHRGKMRGKATRGADVSFGTYGLKAMECGWITS
                     RQIEAARIAMTRYIKRGGKIWIRIFPDKPVTKKPLEVRMGSGKGAPEFWVSVIKPGRV
                     MFEIDGVTEELAKEAFRLAAFKLPLKSKFVTRGQVHA"
     gene            complement(17969..18622)
                     /gene="rpsC"
                     /locus_tag="PK937_08320"
     CDS             complement(17969..18622)
                     /gene="rpsC"
                     /locus_tag="PK937_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNH32613.1"
                     /translation="MGQKTNPTGFRLGVIKTWNSSWFDEKNFADKLQEDLTLRNYLRK
                     RLPKDARVTKIAIDRIKKETEVITITIHTARPGIVIGRKGKDVDQLREELKKITGKEI
                     RLNISEIKVPEVDSYLVAADIARQIESQIPYRRAMKNATRAAMRMGAEGIRVAMSGRL
                     GGAEMARADRYAEGRVPLHTLRADIDFAYGTAQTTYGQIGVKVWICKGEVLDTMETK"
     gene            complement(18661..19002)
                     /gene="rplV"
                     /locus_tag="PK937_08325"
     CDS             complement(18661..19002)
                     /gene="rplV"
                     /locus_tag="PK937_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNH32614.1"
                     /translation="MMEAKAITKYLRISPRKVRRVAELVRGKNTEEAISILHFTRKNA
                     SEHIEKTVRSAVANIMQAKPGADTHSLFVKEIFVDGGPMLKRGRAGSMGRRSMIRRRT
                     AHITVVVAEKA"
     gene            complement(19030..19323)
                     /gene="rpsS"
                     /locus_tag="PK937_08330"
     CDS             complement(19030..19323)
                     /gene="rpsS"
                     /locus_tag="PK937_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNH32615.1"
                     /translation="MSRSIKKGPFLDENLIGKVEVMNKNKDRKVIKTWARACTIAPDF
                     VGHTFAVHNGKNFIPVYVTENMVGHKLGEFAPTRTFRGHSGSKSAAAATAPKK"
     gene            complement(19377..20204)
                     /gene="rplB"
                     /locus_tag="PK937_08335"
     CDS             complement(19377..20204)
                     /gene="rplB"
                     /locus_tag="PK937_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNH32616.1"
                     /translation="MAVKNFRPYTPSRRYITVADFAEITKTEPEKSLVEPKKRVGGRN
                     NIGHTTSRFRGGGHKRMYRIIDFKRHRTGVEAKVVSIEYDPNRSANIALIQYVDGQKA
                     YILAPKGLQVGEKVMSGAEAEIKLGNAMPLKGIPVGTDVHNVELKLGKGGQMARSAGA
                     FANVVGREEGYVQLRMPSGEIRRVNENCFATIGQVGNLEHENVTLGKAGRKRWLGRRP
                     HNRGVVMNPVDHPHGGGEGKSPQGNPHPVTPWGQPTKGYKTRKKNHPTNRYIIKRRK"
     gene            complement(20266..20595)
                     /gene="rplW"
                     /locus_tag="PK937_08340"
     CDS             complement(20266..20595)
                     /gene="rplW"
                     /locus_tag="PK937_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNH32617.1"
                     /translation="MNITQINKILIRPVITEKMTILQDKKKRNGEPLNQYGFIVEKTA
                     TKTDIKKAIEKHFNVKVKSVRTVNVEGKSSVRYTKRGFVQGKRADRKKAIVTLAKDNK
                     IEFVEGA"
     gene            complement(20619..21335)
                     /gene="rplD"
                     /locus_tag="PK937_08345"
     CDS             complement(20619..21335)
                     /gene="rplD"
                     /locus_tag="PK937_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNH32618.1"
                     /translation="MKIEVKNIKGEATGRSVELPKDIFGIEPSEHAVYVAVTHELSNK
                     RHGTHSTLEKSEVSGTTKKPWKQKGTGGARSGSVKTPLWPGGGIVFGPRPHLYKKDTN
                     VKLKRLARKSVLSIKASENRIVVVEDFHTLCADLPKTKEVYGVLNNLQLTGEVTVDNG
                     KRTKPFQKVLVLVDKTGLVKDEKSAKVYENFRKSCRNIPGLKVGVVNNVSTYELMNAD
                     TILFHESAIKNVNAAFGAAE"
     gene            complement(21368..22006)
                     /gene="rplC"
                     /locus_tag="PK937_08350"
     CDS             complement(21368..22006)
                     /gene="rplC"
                     /locus_tag="PK937_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNH32619.1"
                     /translation="MSSGMIGRKIGMSNIFDESGNIIPVTLIEALPNVVTQIKTKEKD
                     GYEALQLAVGDKRGKRTNKAIQGHAKKANYTEKFPDVFREFDGFNVNDFTLGAEVKLD
                     IFAEGEKVTVIGISKGKGFQGVVKRHGFGGINETTHGASDRPRSPGSIGGSSDPSRVF
                     KGMRMAGRMGSDRVTIKNLKVVRLDKESNVLFIKGSVPGATNSYIEIRKNAK"
     gene            complement(22095..22415)
                     /gene="rpsJ"
                     /locus_tag="PK937_08355"
     CDS             complement(22095..22415)
                     /gene="rpsJ"
                     /locus_tag="PK937_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNH32620.1"
                     /translation="MTGQGIRIKLKAYDHNLIDKSIDKIIRTAKSSGAVISGPIPLPT
                     DKTIYTVLRSPHVDKKSREQFETRIHKRLICILNATNKTIEALSKLELPAGVDIELKV
                     TANS"
BASE COUNT         5366 a         5539 c         4927 g         6704 t
ORIGIN      
        1 cgttgaccaa aaaactgcgc cccacggcgc cccacggcga acggaataac gtatcccccg
       61 taaaagtcat ccgcggcact gatttttttt cgtttccatg tggctttccc aaaagaaacg
      121 ttcgcaacga atcgcgactt acctgcggca aaacggtcgt agccgacaca ttggaatcta
      181 aagtttgacc gtatatcggt gcgccaaaga acaataaaaa tatgcacaaa gtgacatcca
      241 aaagcaatgc ccacggcggc gatacgcgct tattcatggg ctagctgccg tatatgagag
      301 acgaccgatg cgcccaatgc ttcaagatga taaccacctt ccaatacaga aatcacgcgc
      361 ccttgacaat gtttttctgc cgcctgcacg actaaacggg ttaattttcc aaatccctcc
      421 gaagtcaccg tcatattcgc caagggatcc cgggcatgtg catcaaatcc tgctgagatg
      481 aataaaagct ccggtttgaa ttgatccaat gccggtatga tttgtttatc aaacacggtc
      541 tccatctcca tatcaccatc accggcatgc ataggtatgt tgagcgtgta acccttaccg
      601 gctccgttgc cgatttcggt atgctgacct gtgccgggat acaagggcga ttcgtgagta
      661 cttataaaaa gtaccgtcgg gtcttcataa aaaatggctt gcgtaccatt accatggtgc
      721 acatcccaat ctacgatcgc aatgcgttgt aatccgtagg cacggcgcgc atacgtcgcc
      781 ccgacagcca cattattaaa caaacaaaaa cccatcgctt tttctttttc ggcatgatgg
      841 cccggcgggc gcaccgcaca aaatgcgcga gtgagctgac cggagcaaac ggcatctacg
      901 gccactacaa cggcgcctgc ggccatacgc gcggcctcat acgaagcatg agcgacgtag
      961 gtgtcgccat caatcatcgc cggcgcgcgg gacgcttctt cttttatacg atcaatatgg
     1021 acttcactat gcacttccac caataatgat tcgtcggcag gttttatgtg taaattttcc
     1081 agtgtgtcat ttaatttggc ctctgcgata gcctgcgtga tggccccgag gcgttcgggg
     1141 cattccggat gatgaccggc atacattcga tgttctaaaa aaataggcgc gtaggcaaat
     1201 ccgattttca tacatcctgt gaagaaaaac tttgtacggc ttcttcaacg gttttgtagg
     1261 ttttaaaaat cttgagaagc tgcgtaatca tcaaaaggct ctccactttt tcaataaccg
     1321 ccgacaaacg caagtcacca ccgtgcagtt tcatcgtagt catgctggac atcagtacac
     1381 caagaccgga actgttcatc cactttacct gactcagatc aagaaccagt ttttttacac
     1441 cgctttcggc caatacatgt actcggtcat gcacagcgct ggtttccatg ccgcccatga
     1501 gtttgccgtg tacgtacacg atagttacat cacccttttt ttcttcttta aacgtcatat
     1561 ccctcgctta cgatgcccgc atccgcagct tgattcgcag cagataggcc atcagtaata
     1621 ataaaacacc aatcaaaaat accatccaaa tttgaatatc cccgcgatcg gctgtactat
     1681 gcgcacgcat cgtcatggat ttatgctcca tcaattcgtc tacatcgtat gtccagtgtg
     1741 ccgtcgcttt gtcaaattca ccgctattca acgaatctat tttcccggga agcgtgatat
     1801 aaaactcaaa tttcgtatcc gccatcagca ttttggcaac cggtaaatcg ttccgtagat
     1861 ccgatcgctc gatttcttcc ttaaacattt tggtgtaaaa atcctcacgg tatgaaaaac
     1921 gcagaccttc tttttcaacc gtgatcgcgc tcagatcgtc ttcgtatttt tcgatacgat
     1981 gcgacgccgt cgggaatact ttttgtgcga ctatggtgag acggtcattc tcattttttt
     2041 cgatcagctc gtatccgttt ttacggaaaa aatctttttt gtatttgacc gcttccttca
     2101 aatcggcatc catactcagt tcgatcgcgc gtgccaccga aaggtcttca tacaccgtcg
     2161 tgcgtatctt ggcatgataa ccgaccaacg tacttcccga ttggaacccg gtagtaaaaa
     2221 tcccggcaat taatattaat acgatgcgct gcatagccct ttcagactcg ttttggagca
     2281 taagttaata agtaatgttg aatatagccg cataaacgga gattatcaat ttgattgtac
     2341 gaagaggtta atgcgtcaaa tcagatttct tccgaggacg tttaacccaa tttgatggtt
     2401 ttgatggctt cgactaattg tttatacgtt tcatcgccga tcgccagtgt atcgccgtta
     2461 tccatcgtaa cactgccatc cgaagctttg cctatttttt tgatacgcga cagattgagg
     2521 atgatggaac ggcctgcacg gacaaaaggg cctgccgatt gcagtttttc atattcggcc
     2581 agatttttgg aaactaaaat agtctcttgt ttcattaata taaatttggt gtatgcgcct
     2641 tctgctttga gatatatgat ctgatcggtc ggtataaaaa caataccgtc accggttttt
     2701 aatgcgattt tattccatgt tcctgctgtt tgttctttga gtacctgata ctgttctgcg
     2761 ttgatttgac cgagggttac tttatccagt gcttgcttca ggcgattggg acttaccggc
     2821 tttaaaatat aatccacggc cgaaagttca aatgcacgta cggcatgttc ggcatacgcc
     2881 gtgacaaaaa ttattttaaa atgaatccgt tcaggagaaa aaaaatcgag tatctctaga
     2941 ccgctgtggc cgggcatttc gatatccagc aataccacgt ccggattcgt tttgtgaata
     3001 agctttacac cgtccggcag cgtacccgct tcaccgacaa tttcaatatc ggtcatgttt
     3061 tccgatatca taaggcgcaa tgtttcacgc gctttgacgg catcatctat gatgattatc
     3121 cgcaggcgac tcatgctctg tcctccaaag gaaagactat atcaacgcgt gtaccttcgg
     3181 ctccatgttc cgacggctcg tcagtaaatt gacacacaat ggaccgcgat tgcagttgac
     3241 tcaataacgc gatgcgttcc gccgtggctt gcgtggcaaa tgagcgcggc tttttgccgt
     3301 tacgcgcatt gatttccgct gacgcggcgc gaccgattcc gttatcttcg atcacaacac
     3361 gcagatcgtt tcccgataaa ttcttttcaa aacgaatgct gagttttttg gaacccgact
     3421 tgtgcagcaa gccgtgtttg atcgcatttt cggcaaaggg ttgtatgacc atagccggaa
     3481 cttcgactgt ataaggatct aatcccggat caacaacgat ctcatactga aaagcatgat
     3541 ccgcacaaag cgcttccaac tccaaataac gggaaagata ttcggactcc tcgtgcagtg
     3601 ttatccattg ccggccgctg ttctccagtg ttttacgcat cagatcggaa aatttactga
     3661 ggttttcact ggcttcgttt ttttgattgg catatacaaa gctctgtatc gaattgagta
     3721 cgttgtatat gaagtgcgga ttcatttggg atttaagtcc cttcaattcg gattgagcta
     3781 tagccacttc cattgcttta cttttttcgg cagcgatcat ctgcatactg agtatttcat
     3841 ttttcttgtg ggcctgtagg gtttgaatat aaaaaaatga agcgcctccg acgacggcaa
     3901 gcagtaatat aattcctacg atcagtatat tttttcgatc gctttccagc gactggactt
     3961 tcaggcgctc ttgctgctcg cgaatagtta tttctttttt ctctgtttca tagcgcgtga
     4021 tgagctcttg cagggatttt gttttttcta tactcaaaag actgtcattg atttgctgat
     4081 actgcatctg cgcctcatag gcctttcgaa aatcgcccgt ttgtaccgcg gcacgtgcca
     4141 aaccttgata cgccgtcatc gcattactca ataatccgga tcgcttggct acctcagcac
     4201 cggcttcaaa gcttagtaca gcttccgccc aacggcccgt ttcgctgtag gcgacaccca
     4261 atccgttatg acatgccgat tctgtatccg ctatacgcaa cgcttgcgct atggtgaggg
     4321 attgacgaaa gctcgtaatc gccttgctgt ataatttcat gttattataa ttcgctgcga
     4381 tattgagcat ggcgatggat gttccataag gtatgttata taccttggcg atcgaatcaa
     4441 ctttttcaaa ccaacggata ccttcggcaa acattttctt attaccataa cacccgccga
     4501 tattggtata caatgccgca taatgaatag ggttagcgct aatcagcggc aaaagttcga
     4561 tctgttggaa gtgaatagcc aacgcttcgt ctacaagttg tttattgttg tagacgattg
     4621 ctatattatt taggataaca aaatcggcgt aatccagacg cgggttacga atgcgatgct
     4681 gtttgacata atccgacgct tgcaaataat tctccatcgc tttcgtcagg tcgccttttt
     4741 ggtagtatgc cataccgcgg cagttgatgg ctgttgcaat tccgtactcg tatccgattt
     4801 ctttggaaat gatcagcgcc tcttcagcgt atatcaaaac gctgtccggc tctttgaatg
     4861 tcaacgcggc acagatatct gtcaaaattt ccacgcgtgc tgaatcacgg ataggataaa
     4921 cgcgcagttt gcgcaacaaa gaatcggcat caacttcttg tgcagtaaga tcacaccgga
     4981 ttaaagccgt aataacgaat aaggaattaa tgatgcgcat gatgttataa gttaatgatt
     5041 aaaattcttg acaagcgttt tatactcttt ctgcgtacgg agtgtctcat aagaaaaaaa
     5101 agataccccg gcaaagccat acttacgaca tattctcaat ttttcttcgg cgctttttac
     5161 cggctgatta tacaaagaaa cacccataat gattttttgg gtatcggatg cgcctaattt
     5221 tattaccgca tcctggatac gtttttcaaa ttctccgtta tccgttgcat aattcatagg
     5281 cacagcaaaa tctaaacagt ttgactttat ccattccggc caatcttgaa gaacacgatt
     5341 ttgggcatcg tcttgtgacg cccatacggc cgctgaaacc gcaatggacg aatcggcttt
     5401 tttgatttgt gtatatatgc aggcaacata atcttcgata attcttttcc taaagcgcgt
     5461 ccaaatactg tcctgccagg catttaatgc ttcggctaaa ggatcccaat gatattctaa
     5521 aataaagcga ctgcgcatgg atgtagaata atcatagtcc gcctcagggt aacggatata
     5581 gtcaaaatga atgccgtcaa ctggatactt agataccaat tcttttacta cagctgaaaa
     5641 ataatcttgg aactcctgga ttgcgggcga gataaaaacg ccttcgagtc gcttggcaat
     5701 tagttttttt gttccttcaa ccgacatgga ctgtccttgt gcattgacag cggcccaatc
     5761 aggttgtgtg taaaaaagat gttcgggatg gaccggattt ttttcggatg accataaata
     5821 aaatacattc agccataaat gaatttttat tttttttaat ctgttctttt cgatcacgta
     5881 ctgcaatgga tcaaacgccg ggtcaaggcc ttcggcttta accacaaaat cggaattata
     5941 atatgcatca ccgcgaccgc gtacttgcaa aaaaacatcc gtgattccgt tttgatcaca
     6001 aaaggacaac atgcgatcaa tttcaatctt agattttaaa atatctcgga ctacccatat
     6061 tgcttttatt ctcgccttct cttgtgctac ggttacgttg gacggcaatc catatgacaa
     6121 tagcacaaag ccaataaata aatatttact taataatttc attcgctatt cggctatttg
     6181 gttaatttcc aatgatcgat tattatacat attggaatgc attttacata ctacaaaaat
     6241 aaaaaaggcg ttcataaata tgaacgcctt ataaaaattt ccgagatcta attaaccgtt
     6301 gtaggtcgtt tcctgataaa actcacaacg acgcttttta cctgaacgca cggttgaaag
     6361 ttttccgtta ggcaaacgtt tagccgcgca ctggtgtgca tgaccggagg tcattagatg
     6421 cgcgcagata gtttttttgc ccggtgcaaa gcattgaatt ttaggcaata atacttcgct
     6481 agccgcttcg gtttttttac ttacggtagc tttagcttcc gttttgactt cttctttttt
     6541 cttacgcgga gccgccgctt ttttcttcgg ggctttcgtt tctttctcgt ccgcagcttc
     6601 ttttttggta cggggcattt ttacctctat ctaaaactta cttggattct ttttgagctt
     6661 cttctttttt tgctttgata cgttcagcca gcgatttggc gcgttgagct ttagcttcct
     6721 tggcttcgat ggctttgttt tgtttatcaa tggaagccgt ttcataaccg actaattcca
     6781 aaacagccat ttccgtcgca tcacctttac gcagacccaa acgcagaacg cgtgtatagc
     6841 caccgccggt ataagatgca tttttagctt tacgctgagc atccaattcc ttcatcttcg
     6901 gtgcgatttc gttgatgagc tcatgaatta cggtcttgga aagttctttt ttatcggtaa
     6961 catgtttatg cgccgttacc aaacgggcgt taccgaggcg actgatcaat atacgacgcg
     7021 ctgccagatc accttttttg gcaaacatga tcaagcgttc ggccgtcata cgagccgctt
     7081 tcgccttgga aatagtcgtc acaatacgtt tatgacggaa aagttcgttg cacaaacttt
     7141 tgatcagagc ctgacggtga cttgccgttt tgctcagctt tatttgtttc ttaccgtgtc
     7201 tcatcgttcc tctaatgcca aattaaaatt attcttcacc gttgtagtat ttgctgacat
     7261 ccataccgaa actcatattg cgttcttcga gaatcttggt taactcggtc agcgacttac
     7321 gaccaaaatt gcgatattta agcatttccg attcgttttt gctgacaaga tcaccgatag
     7381 tcttgatgcc cgctgcacgt aaacaattat gtgaacgcac cgacaattcc agttcatcaa
     7441 cgctcatctt aaggagctga cgaatgcgcg cggcttcctg atcttcgact tcaccgtcgg
     7501 cattttcatc ttccatttca aagttaatga aaagttgaat atggtcgcgg aataagcgtg
     7561 cggcaatagt cagcgcatcg tccggtgtaa tgctgccgtc cgtcgtgact tcgagggtca
     7621 gtttttcata gtcggtatgc tgaccgacgc gtgtactgtc gatagtaaaa cgtacatttt
     7681 tgatcggatt gaaaatcgaa tcaatcggta ccgtaccgat gggcatgtcg ggaaatttat
     7741 tttcttccgc agttacccaa cctttgccgc gaccgatacg gatttccatt tcgacatttg
     7801 cttctttgct gagcgtcgca atgacgtgat ccggattgag tacttcaaaa tcaatgccgt
     7861 tggaagcttt ttggatatcc gccgccgtaa atttgtgcgg tcctttgagg cgaatcgaaa
     7921 ctttatccgg ctttttattg tgcaatttaa agcgcacacc cttgagatta agcaccattt
     7981 ccgcaacgtc ttccgcaacg ccgggaattg tggagagttc gtgaagcacg ccttcgattt
     8041 tgatggcggt gatcgcggca cccgggagcg acgacaagag tacacggcga agcgaattgc
     8101 cgatcgtcac gccgaatccg cgttcgagag gctgaataat aaacttaccg tatgtagtcg
     8161 tgtaggacga ctcgtcgagt tcgatgcgct ccggcatctg aagtcctgcc cagttctgca
     8221 ctatagaatc tcctcattta gagattagac gttttatata aaaagcgtcg tattcgttat
     8281 tgaattactt cgagtacaac tcaacaatga gctgttcttt gacatccaca gggatctgtt
     8341 cgcgttcagg catattcaga aatttgccct tgagcgaagc tttatccacg tcaatccagt
     8401 tcagggatgt ttctttttga cgtttcagtg atccgtggat caacgccatt tttttgctgc
     8461 cctcgcgaat agacacatcg tcgccgggtt tgacctgata cgaaggcgcc gttacgagtt
     8521 tgccgttaac ttcgacatga cggtgcagta cgagctgacg cgcttgcgaa cgcgaagaag
     8581 caaagccgaa gcgatatacc atgttatcaa gacgtgtttc aaggatttga agcaacgccg
     8641 taccggttac acctttagca cgttctgctt tttcaaagta gcgacggaat tgacgttcca
     8701 atacgccgta aatacgacgc agtttttgtt tttcacgcag ctgaatgctg taacccgagg
     8761 gttttgaacg tttgtcgccg tgctcaccgg gggggtagtt gcggcgttcg atcgggcatt
     8821 tgtccgtcaa gcattttgtg cctttgagaa aaagcttttc gccttcgcgg cggcacagtt
     8881 tgcaaacggc atccgtatat ctggccatta agtatctcct aaaattgtat cttcagtcgt
     8941 gttttatacg cggcgtttct tcggcggacg gcatccattg tgcgggatag gcgtcaagtc
     9001 gcggatcgcc gtaatctcca aacctgccgc ttgcaacgaa cgaatcgctg actcacgacc
     9061 ggcaccgggg cctttgacgc gcacttcgac ttttttcaat cccatatcaa ttgccgtttt
     9121 agcggccgtt tcagcggcaa cctgtgcagc gaaaggcgtg ctctttttgg aaccgcgaaa
     9181 acccatcttt ccggatgtac accacgaaat cacttcaccg tggctgttgg tcaaagttac
     9241 gatcgtgttg ttaaacgttg cacgaatcac ggcaacgccc gtggattcga tcagtttctt
     9301 ctttttacgc ggcaatgcgg cagcctgttg ctgctgcgga ttttgcttgg ccaaagttct
     9361 ctccttggat caaaaattca attacttctt tgcttcgact ttcttgagac cgccgatcgt
     9421 cttacgttta cctttacgcg tacgggaatt ggtacgtgta cgttgaccgc gcgcgggcag
     9481 accgcgacgg tgacgcagac cgcggtagga accgatgtcc atcaaacgtt tgatgttgag
     9541 cgcattttcc gaacgcagtg cgccttcaac tttgatatta tcattgatga tcgtacggat
     9601 cttgttttct tcatccggcg taagatcttt gatctttttc atctcatcca caccggcttt
     9661 tttcaaaata tcttttgccg tagtgcggcc gatgccgtag atgtacgtca acgcaacaaa
     9721 tgcgcgtttg tctttgggta attctatacc tgctatacgg gccaaagcgg ttctccctaa
     9781 aatttaaata ttaaccttga cgctgtttgt gttttttatt atcgcaaatc acgcgaacaa
     9841 cacctttgcg acggatgatc ttacacttta cacaaatttt acgaacggac gatctgactt
     9901 tcatatcgta actccattat ttatagcgat acgtgatgcg cccgcgggat aagtcgtacg
     9961 gtgataattc aatcgttacc ttatcgccgg gcagaatccg aataaaattc atacgcattt
    10021 ttccactgat gtgcgcatga acctggtgac cattttcaag ttttacaata aacgtggcat
    10081 taggcaaagt ctcaagtatc gtcccgtcaa ctttgatcaa tccttctttt gccaagtttt
    10141 aactccgttc taacgtaagt tattgtaaaa acaatcccac aaatgggcta ccaatatata
    10201 aaaaaaaatt aaaaaatcaa tagctaattc taaaataaat cggactgtgt taaaatttcc
    10261 ggtccgtttg atgttactac taccgtatgt tcaaaatgcg ctgacggttt cccgtctttc
    10321 gtatggatag tccaattatc ttttgatgta tacacttcat atgttcctgc attgaccatc
    10381 ggctcaacac aaaataccat accttctttg agtcgcgctc cggtaccggc tttgccgaaa
    10441 tttggaacct gaggatcttc gtgtaattcg cgtcctatcc cgtgaccgac taattcgcgt
    10501 acaactgaaa aaccatggtg ttcaacgtat gtttgaacgg cggctgaaat atcatgaact
    10561 cgattgccgg gaaccgcttt ttgaataccc aaatacaaag attctcgcgt cacttgaagt
    10621 agctttttct tttcttcgga gatttcgccg accggcaatg ttacggcgga gtcaccgtaa
    10681 aacccgtttt tgagaacacc aatatccaca ccgagtatct gaccttcctg cagtatgtcc
    10741 ccgggtttgg ctataccgtg aacaacaata tcatcggtcg aaaggcaagc attattggga
    10801 aaaccatgat aacctttgaa cgccggtttc ccgccgcgcg atttgatagc cgattcgata
    10861 gccgcatcca aatccatcaa acgcaatccg ggcttcacca tcttggccgc taccatcata
    10921 gcttctacga taatacggca gctttcgcgg attttatcaa tctccgcacc cgacttgagc
    10981 cggatcattc ggattatccc cgacggccgc ggatacgacc ggacttaagg aaaccgtcat
    11041 agtggcgcat gacgagatgc gattcgattt gctgaatcgt atccaatccg acacccacca
    11101 cgatcaaaag cgacgtgccg ccaaaaaacg acgcgaagct atagctcaca tcggttgcat
    11161 ggcgaacgaa aaacggcaat atcgcaatga caccgaggaa aatggcgctc ggaagggtga
    11221 cgcgcgtcag gatattatca ataaaatccg acgttttttt gccgggacga acacccggta
    11281 taaacgcacc ttgcttcttg aggttatccg ccacttcgac aggattcatc gcaatcgcag
    11341 tgtagaaata cgtgaagatg acaattaaaa tgagaaagaa caaattgtac gtaatcgaat
    11401 caatcgaaaa atgtgctgtg atccactccg aaaaatcacc cgggaaaaaa gtggctaccg
    11461 tattcggaac aaacatgatg gactgcgcaa agatgatagg cataacaccg gcaatattga
    11521 tacgcatcgg gatatgtgtg gattgaccgc catatacctt acgaccgatc acgcggcgtg
    11581 catattgtac cgggatttta cggatacctt gcgtgataaa aataatcgcc gcaacaatac
    11641 cgagtaaaat tgttaccaaa atcaattcaa gaataatatt gcgattgcct gtcgaaattt
    11701 gagtgatttc gtcaataatc gccgtcggga atcgcgccaa aataccgatc gcaataatca
    11761 atgaaatacc gtttccgatg ccgcgttcgg tgatctgctc gccgagccac atgataaaca
    11821 tcgtacacgt agtcatggtg accatcgtca tcagataaaa agtgaagcct tgatccggca
    11881 ctactttgac ttcctgactg ccgatcacta cactgagact gcaaagccag gttgcaacgc
    11941 cgatcgcctg aacggcggca agcgctaccg taccgtagcg tgtaagctga ttgatttttt
    12001 tgcggccttc ttcgccttca cgttgtaaac gctgaaggta aggcactgtc gtaccgagaa
    12061 gttggatgat aatggatgta ctgatgtacg gcatgatgcc aagcgctgcg atagtcgctt
    12121 gagacaatcc gccgccggca aataaatcat aaaaactcaa cagcgtattt tccgcctgac
    12181 ggaaatactg tccgagcgct tccgcataaa tacccggagc gggaatgtgc gccgcaatac
    12241 gctcaatcag caaaacgccg agcgtaaaga ggatacgttt ccgcaattcc ggaattttaa
    12301 atacattcgc aaatgtttgt agcattgtga aattctcttg ggcttatgta agagtaaccg
    12361 ctatttcgac gcttttttct tgcgtttttc caacactgtt tttttgatgc caagcaccgt
    12421 caccttgccg ccggcttttt cgattttctc ttttgccgtt gcactgatcg catgcacttc
    12481 gacttcaaac gctttggtca cttcaccggt tcctaaaagt tttaccggag cgcttttctt
    12541 ttttatcaag ccagccgctt gcagcgttgc gccgtccacg cgtttgttat ccaattcggc
    12601 aagatcggcc aaattaacag tctgaaattc gacacggaaa atattggtaa aaccatattt
    12661 agggagacga cgttgcaaag gcatctgtcc gccttcaaaa ccggcaaggc ggttaagacc
    12721 ggaacgcgac ttttgccctt tgttaccgcg tgtggatgtg ccgccgtggc cggagccttc
    12781 accacgaccg atacgtttca ttttacggcg ggagcctttg gcgggcgtta aattactgag
    12841 attcatcgca atcctctttg actaaaattg tttacgcttt tttcgctttg gtaactttat
    12901 aaccggtctt tttttctttt ttcacagctt ccggtttttt atcaacggct tcacaggaaa
    12961 ccatatactt cacaagatta accataccgc gaacctgcgg tgtgtcgttg tgataaacgg
    13021 tttggccgat tttacgtagt ccgagcgtgc gcacggtatc gcggtgtgcg aaaattgcgc
    13081 cgttggtact gcgaacctgg gtaatcttca aaaatttcat gatcgctctt tgcctggtta
    13141 tcgttacttg gtaaatacat ccatgacagt catgccgcgg acggcaccga cttcgcgcgc
    13201 attggtcact tcactaagcg ctttcatcgt ggctttgacc acattgtgcg gattggaaga
    13261 acctttgcat ttggtaagaa tgtccgtgat gccgacggat tcaactacgg cacgtatcac
    13321 ggcgccggct ataataccgg tacctttagt tgccggtttg agcaacactt tgccggcgcc
    13381 aaacttggcc gacacttcgt gcggaagcgt accaccgtgc aacggtacaa atatcatacg
    13441 tttttttgcg tcttctttgc ctttggctat agcatccggc acttcgttag ctttacctaa
    13501 tccaataccg actttgcctt taccgtcgcc gaccaccacg atagcgttaa aactgaaacg
    13561 gcgtccgcct ttcactactt tcgctacacg gttgatatgt acgacgcgtt cttctttgag
    13621 ttcgacgtcg gccggattat cctgggcttc ttttttcttg atttgtttcc tattattcat
    13681 cacgcctcac catctataga tgtgtttttt attacaattt caatccggct tcacgagcgc
    13741 cttcggcaac cgctttgatg cggccatgat atttgtatcc attacgatca aatacggcga
    13801 cttcaatacc tttttctttg gccaatttgg cgatagacat accgaccgct ttgctcaacg
    13861 aaactttacc gctctctttt tgtatgcctt tggtcttcga cgaaacctgt gcaatcgtat
    13921 gaccggcttc atcgttgatg agctgagcgt aaatgtgatt gaggctgcga tacaccgaca
    13981 accggggttt ttccgccgtg ccgatgatgc gtttacgaat acggtatttg acacgttgtc
    14041 tttttaccgc tttgactatg tttttgtccg ccatggtgct tttaacaatt tatagtttta
    14101 aaaattactt cttggtcgcg gccttaccgg ctttacgacg gatggtttca tccgaatatt
    14161 tgataccttt acccttatac ggttccggca tcttgaaaga acgaattttc tgagccactt
    14221 gaccaaccaa ttctttattg atacctttga tgcttacgct ggtcggcgta ggaatcgcaa
    14281 tttcaacacc tgtcggagga gcgaaaagaa tcgggtgcga aaaaccgaga ttaaattgga
    14341 ttactttgcc tttcgcttcg gctttaaaac cgacgccgat aatatccaat ttacgttcat
    14401 atccatcgga cacgcccgtc accatgccgg caatgatcgc gcgaacgctg ccgtgcaacg
    14461 cacgctgaaa attggtgttg tttttacgcg aaacgatgat ttctttgtct ttgacttcga
    14521 ccatcgcttc agggtgaaac tgtttggtca gttcgccctt ggggccttta acggtgacga
    14581 ctgaaccttt gacggtcact gtgaccttat cgggaatcgc taccggtttt tttccaatac
    14641 gtgacactgc ttatactcct gcgaataaaa tttcgttcag attacgatac gtaacacaat
    14701 acttcgccgc cgacattttc cgttttggcg cgtttgttgg tcatcacacc tttggatgtg
    14761 gacatgatgg caataccgag attgcttttt acgcgcggca gcttatctac acccgcatac
    14821 ttccggaggc ccggcgtgct ggcacggtca atctgattga tcgcattgcg gtttttctca
    14881 tcgtatttca agaaaatacg aatgatgcct tgtacgccgt catcatagtt agcgtagtct
    14941 ttgatatatc cctgttcaag caggatttgc gtaatatcgc gcttcaggtt agaagctttg
    15001 atatccacga atttcttttt ggcctgtatc gcgttgcgaa tcgccgtcaa ataatccgcg
    15061 atcggatcgg tcattgacat cggatcgtct cctttgataa aaaataaaaa ctcaaaaatt
    15121 ctctactatt accacgaagc tttacgaatg cccggaatct caccattgag cgaaagatta
    15181 cggaagcata tacggcaaat gccgaacttg cgcatataag cgcgcggacg gccgcagcgg
    15241 cggcaacgat tgcgcgtgcg tgtggaaaat ttcggtgttt tcttcgtctt agcaacccat
    15301 gctttcgtag ccaaggttca aactcctgtt agtatcgttc gtgttatatt atttcttttt
    15361 gaacggcata ccgaactctt tgagcagttc gtatgcttct tcgtccgttt tagctgacgt
    15421 cacgatggtg atgtccatac cgcgtacgcg gtccatttta tcaacatcga tttccggaaa
    15481 aataatctgc tctttgacgc ctaacgtata gttaccgcga ccgtcaaacg atttatcggg
    15541 aacgcctttg aaatctcgaa tacgcggtac ggcgatattg atcaaacgat caagaaattc
    15601 gtacatcgcg gcgcgacgca aggtgacaaa gcaagcgata cctacgcctt cgcgtaattt
    15661 gaaattggaa atagcttttt tcgctttgcg gatagtgggt ttctgacccg tgatgatcaa
    15721 aagatcattg accgccgcat ccaaaagttt cggctcttgt gtcgccatac cgacgcccat
    15781 attgatcacg actttttcga tcttagggac ttgcatgata ttcttatagc tgaatttttt
    15841 catcagcgca ggaaccacac gtttacggta ttcttcaccg aggcgggctt tgtagtcttt
    15901 acctacggcc gcacgttttc cggaaggtat ttctacaccg gttgatgccg ctttatcttt
    15961 gccttttttc tctttcgctt ccttcgccat ggctctaacg ttcctctaaa attataatac
    16021 gtcttaattg atcacttcgc cggatttttt ggcaatgcgc actttggatc cgtcttccag
    16081 ttttttcgca cctgtacgcg tcggcttgcc gccatgtaca agcatgactt tgcttgccgg
    16141 aatcgcattt tctttttcct gaatgccgcc ttgcggattt ttcggattcg gcttaatgtg
    16201 tttcttgcgt aaattaatac cttcgacgat caccttaccg gtatccgtga ggaccttcag
    16261 gactttgccc tgtttgcctt tgtgcacgcc ggagatcacg gcgacaagat cattcttttt
    16321 gatatgcatg atgtaaaacc tttgatgttt cgcttattcg ttcgcgctta gattacttcc
    16381 ggcgcgaggg atacaatctt catgaattgt ttttcacgaa gttcgcgtgc gaccggtccg
    16441 aaaatacggg ttccggtggg ttctttatct ttattgatca gcacggcggc gttctcgtca
    16501 aagcggatgt atgaaccgtc tttgcgacgc atttccttgc gtacacgaac tactaccgca
    16561 cgagcgacag cgcctttttt gactgtgccg ccaggaatcg catctttcac agacacgatg
    16621 atttcatcgc cgaggaaagc atagcgtttg gaggtaccgc ccaatacgcg gatacacatc
    16681 actttttttg cgccggagtt atcggcaacc gtcaatcggg taaactcttg aatcatgact
    16741 tatctcgcca gttaataaat gacctgaatt aatccgcttt tttcacaact tccaccaagc
    16801 gccaacgttt atttttgctc agcgggcggg tttccattac ttttacgata tcgccgatac
    16861 cggcttgctc ttgttcgtca tgcgccatca gcttagccgt ctttttgata tactttttgt
    16921 acgtcttgtg caatactttg cgctcaattg cgacaacgat gcttttgctc atcttatcgc
    16981 tgacgacggt accgatacgc accttgcggc gatttctttc cgtggtctta ttttccgctt
    17041 gttcggacat ggaatgcttc tccaatacga tttatactta tatctaaagt ttttccttaa
    17101 ccgtgcgcga tctctttgag cgctttgtat tccttgacaa aaagggaacg tttgggattg
    17161 gactccaaaa gacgtaccgc tttacgcaaa gccgctttgt cggtattgag tttttcaccc
    17221 ttgagcgtcg gaataccttt gatctgatgt ttacttacca cggcttccag tattttcgcc
    17281 gcttccgcca attcggtttt caacgtttct tcccgcatga cggttttaac gatggcaatt
    17341 tcttttttgg tcgaacgcag atccgttttc ttttccagtt ggcctaccgc attggtaaag
    17401 cgcatcttga ccagattggt ttccgcttcg ttgatcatgc cctttaattc ttcggcgggc
    17461 aatcctcgta cttcatgtgc tttcatgata tctgtctctt ccggttataa ttacgcgtga
    17521 acttgaccac gggtgacgaa tttcgacttg agcggaagtt tgaatgccgc caaacgaaat
    17581 gcttcttttg caagctcttc agtcacaccg tcgatttcga acatgacacg gccgggtttg
    17641 atcaccgaga cccaaaactc aggcgcgcct ttaccggaac ccatacgtac ttctaaaggt
    17701 tttttcgtaa ccggtttatc gggaaatata cgaatccaaa ttttaccgcc gcgtttgata
    17761 tagcgtgtca tcgcgatacg cgccgcttcg atctgacggc ttgtgatcca tccgcattcc
    17821 atagctttga ggccgtaggt accaaagctc acatccgctc cgcgggtagc tttgccgcgc
    17881 attttgccgc ggtgatgtct tctaaattta accttcttag gttgtaacat gtatcagttc
    17941 tcctggaaca aaatttctct gattgagcct acttggtttc catcgtgtcc agcacttcgc
    18001 ctttgcagat ccacactttg acgccgatct ggccgtaggt ggtctgagct gtgccatatg
    18061 caaaatcgat atctgcacgt aaggtatgca acggtacgcg gccttcggca tagcggtcgg
    18121 cgcgtgccat ttcggcgccg ccaaggcgac cggacatggc cacacggata ccttcagcgc
    18181 ccatacgcat cgcggcgcgt gtcgcatttt tcatagcgcg gcgataggga atctggcttt
    18241 cgatctgacg tgcaatgtca gccgcgacaa ggtacgaatc cacttcggga actttgattt
    18301 cggaaatatt caaacgaatt tctttaccgg tgattttctt aagctcttcg cgaagttgat
    18361 cgacatcttt gcctttacga ccgatgacaa tacccgggcg tgcggtgtgg atcgtgatcg
    18421 tgatgacttc ggtttccttt ttgatgcggt caatggcgat tttcgtcacg cgcgcatctt
    18481 tgggaagacg tttgcgcaga taattacgca acgtgagatc ttcctgaagt ttgtcagcaa
    18541 agtttttttc atcaaaccag ctcgagttcc aagttttgat gactcccaac cggaaaccgg
    18601 tcggattcgt tttctgtccc aaagtattcc tccgttaaac ttgtatcgtt aaacgatcaa
    18661 ttacgctttt tccgcgacga ccacagtgat gtgggctgta cggcggcgga tcatcgagcg
    18721 gcgtcccatc gaacccgcac ggccgcgttt gagcatcggc ccgccatcta caaaaatttc
    18781 tttgacaaat aagctgtgcg tatcagcacc cggtttggcc tgcatgatat tggccacagc
    18841 ggaacgcacg gttttttcaa tatgttctga ggcattttta cgggtgaagt gtaaaatgct
    18901 gatcgcttct tccgtatttt ttccacgcac aagttccgca acgcggcgca ctttacgcgg
    18961 cgagattctc aaatatttcg ttattgcttt cgcttccatg atcaggctcc agttctaaaa
    19021 tttgcgcgtt tactttttgg gtgcagtggc ggccgcggcg gatttactgc ccgagtgacc
    19081 gcgaaatgta cgcgtcggtg cgaattcgcc aagtttgtgg ccgaccatgt tttccgtcac
    19141 gtaaaccggg atgaagtttt ttccgttatg tacggcgaag gtatggccga caaagtccgg
    19201 cgcgatcgtg caggcgcggg cccaggtctt gatcactttg cgatctttgt ttttattcat
    19261 aacttccact ttgccgatga gattttcatc gagaaaggga ccttttttga tcgaacgact
    19321 catgtcttat attccttaat tcaaaaaatt cttcgttgtt gcgtcatccc gccgtattac
    19381 ttgcggcgtt tgatgatata ccggttcgtc gggtgatttt tcttacgcgt cttataaccc
    19441 ttcgtcggtt ggccccacgg cgttaccggg tgcggattac cctgcgggct cttaccttca
    19501 ccaccaccat gcgggtgatc cacgggattc ataacgacgc cgcggttgtg aggacggcga
    19561 ccgagccaac gtttacgacc ggctttaccg agcgtgacat tctcgtgttc gagattgccg
    19621 acctgaccga tggtagcaaa acaattttca ttaacgcggc gtatttcgcc cgagggcata
    19681 cgcagttgga catatccttc ttcacgaccg acgacattgg cgaacgcacc ggcagaacgc
    19741 gccatctggc cgcctttgcc gagcttcaat tcgacattgt gtacgtccgt acccacggga
    19801 attcccttga ggggcatggc gttaccgagt ttgatttcgg cttcggcgcc ggacattact
    19861 ttttcaccga cttgcaaacc tttgggtgca agaatatacg ccttctgtcc gtccacatac
    19921 tggatcaacg cgatattggc gctacggttc ggatcgtatt ctatcgaaac aactttggct
    19981 tcgacgcccg tacgatgacg cttgaagtca atgatacggt acatacgttt atggccgcca
    20041 ccacgaaaac gcgatgtcgt gtgaccgata ttgttacgac cgccaacccg ttttttgggt
    20101 tcgacgagcg atttttccgg ttccgttttg gtgatctccg caaagtccgc aaccgtaata
    20161 taacggcggc tcggcgtgta cggtctgaaa ttttttactg ccatgttctt ctctcaactt
    20221 atgccagata ggttttcctg gcgtttctta aacttttcgt tgtaattacg cgccttcgac
    20281 aaattcgatt ttgttgtctt tggccaatgt tactatcgct tttttacgat cggcacgttt
    20341 gccttggaca aaaccgcgtt tggtataacg tacgctgctc tttccttcga cgttgaccgt
    20401 acgaacggat ttcactttga cgttaaaatg tttttcgatc gcttttttga tatcggtttt
    20461 cgtagcggtt ttttcgacga tgaaaccgta ttgattcaac ggttcgccgt tacgtttctt
    20521 tttgtcctgc agaatcgtca tcttctcggt aatcaccgga cgaatcagga ttttgttgat
    20581 ttgcgtgata ttcatgatca gtctctagtc cttatcgttt attctgcagc gccgaatgcg
    20641 gcattgacat ttttgatcgc actctcgtga aaaagaatgg tatccgcatt catcagttcg
    20701 tacgtcgaaa cattgttcac cacgccgact ttaagtccgg gaatattacg gcatgatttg
    20761 cggaagtttt cgtacacctt agcgcttttt tcatctttga caagacctgt tttatcaaca
    20821 agcacgagta ctttttgaaa cggtttagta cgtttgccgt tatctacggt cacttcaccg
    20881 gtcaattgca gattatttaa aacgccatag acttctttgg tcttcggaag atcggcgcaa
    20941 agggtatgaa aatcttcaac caccacaatg cgattttccg atgctttgat actcaataca
    21001 ctcttacgag ccaaacgttt gagtttgaca ttcgtgtctt ttttataaag atgtggacgc
    21061 ggaccgaaca cgataccgcc gcccggccaa agcggggttt tgacggaccc ggagcgggcg
    21121 ccgcccgtcc ctttttgttt ccacggcttt ttggtggtac cggaaacttc cgatttttca
    21181 agcgtgctat gtgtaccatg acgtttattc gaaagttcgt gcgtcacggc aacataaacc
    21241 gcatgttcgc tgggttcgat tccgaaaatg tctttcggta attcgacgct gcgaccggtc
    21301 gcttcgcctt tgatattttt gacttcaatc ttcattgcca gagccttcta tcaaaaatgt
    21361 taacttctta cttcgcgttt ttgcgaatct caatgtacga attcgtcgca cccggcacgc
    21421 tacccttgat aaagagtacg ttggattctt tatccagacg aacgaccttc aggtttttga
    21481 tggtcacgcg atcactaccc atacggccgg ccatgcgcat gcctttgaaa acacgggaag
    21541 gatccgagct accgccgatc gaacccggtg aacgggggcg atccgaggcg ccgtgtgtgg
    21601 tttcattgat accgccgaag ccatggcgtt ttacaacacc ctgaaaacct ttacctttgg
    21661 aaatgccgat cacggtgact ttttcgcctt cggcaaaaat gtccagtttt acttccgcgc
    21721 cgagcgtgaa atcattgaca ttgaagccgt caaattcacg aaacacgtca ggaaattttt
    21781 ccgtgtagtt ggctttcttg gcgtggcctt gtatggcttt gttggtacgc ttgccgcgct
    21841 tgtcgccgac agccagctgc aacgcttcat aaccgtcttt ttctttggtc ttgatctgcg
    21901 tcacgacgtt cggaagcgct tcgatcaacg tcaccgggat aatgttcccg ctctcgtcga
    21961 agatattgct cattccgatt tttcttccga tcataccgct gctcatgtat atactcctcg
    22021 taagaaacag gatgttttcc tgttccatcc aaattgatat ctaaacttta gccaatatta
    22081 acttgatcaa gtgatcagct attggcagtg accttcaatt caatgtccac gcctgccggc
    22141 aattcgagtt tggacaacgc ttctatcgtt ttattcgtcg cattcaagat acagatcaat
    22201 cgcttgtgaa tacgcgtttc gaactgttcg cgggactttt tatccacgtg cggcgaacga
    22261 agaaccgtat aaatcgtctt gtcggtaggg agcggaatcg ggccgctaat cacggcgccg
    22321 gagcttttcg ctgtgcgaat aatcttatca atcgacttat caatcagatt gtgatcgtaa
    22381 gctttcagtt tgattcggat tccttgtcca gtcacttttg cattctctcg ttaattttat
    22441 ttttccgcgg gcattgcttt gggcgacacc cgcggtcaat cttttcttct tacttgagga
    22501 tcttggttac tacaccggcg cctacggtac gaccac
//
LOCUS       DAOQGF010000048        22092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1080_length_22092_cov_8.951990, whole genome shotgun sequence.
ACCESSION   DAOQGF010000048 DAOQGF010000000
VERSION     DAOQGF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 22092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22092
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_22092_cov_8.951990"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5692)
                     /locus_tag="PK937_08360"
     CDS             complement(<1..>5692)
                     /locus_tag="PK937_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH32621.1"
                     /translation="ASYNGLSDETGLITVIGGAPDSIYEISSTSVSLTPTQTTTLKVK
                     LTDDDGNGLQDSVVYWTIQTGSGALSLASDSTDELGVSQVVFTAPTTSGVSTVRAYIT
                     SLSTDSGRFTITTNPAVLAYYTIIPSTNNDTVGVAITATVTAYDIFNNTLDDDTTRVQ
                     FDVIGSVTGLNGSGVGINPTNDTLTNGQLTVVVTDSVKETLQIRVTSLLDTTKRTATN
                     LITLRAAAPSIVEFYPDSLTNVQSGQFGQPLLDSLALVVKDQFGNTVNDTVTVQWFAT
                     SDGTASPVIRTTDNDGIARSLWTLRTGASSYDTMYAVIPVTGDSLQFIANLLPTGEDT
                     LIRVSSLVNSDTVNQMLPDTFRVLVRDQFSNPVSNTAISFTAYSYPSGSDSTGFIRGA
                     GNYVTTLDTVTDINGYASAIFHMGTKVGQYIIRASNPSLLNPAVFDTIQATFGLPGEL
                     RLLSGNAQTDTVDQIVDTLRVQVYDRYGNVVNDTARVIWEAINNSVSVLNQGLVDAVS
                     IDSVSGTPTFRDSALAFTQGIAKANWRLKDLTGSDSVRAFIPGIDTVLFTATTVPDAP
                     FAIIKTEGDSATVVPLGGLVTLKTRLEDQFGNVISGKYVRQNVLAGSVIFVGGDSAMS
                     NAQGISLIELQLGGGDSATVQATTSGLVGGPIFTTYALRYVVGSLKPTIALADTEATF
                     TAAFINYGPHPVALDSNQTRVRFSDGVTTFRSLLSYGDTVIRAHGQDTLQFRTDTIPS
                     SFGGKQFNVRFDLRGQVASVSDTLYGTLETGANVLNILGVDVISVNTPAPKVYGRGDT
                     ITVQLQVYNSSNVTLTNLNYGLLTSKSGVFDLISSIDATPFAPRSTSVFNITAVVRAS
                     APLGQIVVNGFYSGSNGGVGVSDTLANTMDTIIIQSDASLRYVAATLSPDTVSEGQSV
                     AFRISVINDSLASVILDKANTYLRFGGDSTYLTSSQVVPGSGASTTLLFDPLTITNAT
                     SGRYQGVLVLNGTENGGLFDTTLFTGADSLTVQTTVTTASLEFDSVEIALDTTAQYRD
                     SVALRVRIVNNAQASAVIDSIILDATRNLATISGYSVVGTGSLPDTIAGNSSSVFTLY
                     ALISSVADTGSIVFDGRIVAHDINSGNTVNLSPVTDKDSIFVRKRATLSIISVVSPSH
                     DTVAVGQNNIPFVVTIANQGGSPATISDLQLVFKRGLYDTTLTQTLPDTLYGYETRAY
                     NFTAHVLSNSATGLDSIGAYVSGSDQLNQRMISLTSTGRDTLLILSNSLIALNSVQTT
                     PTLVSRGQDSILVRVSVTNLSSSVGRIDTIQLKFFDEAVSLLNTGFDVTPYSDLSDTV
                     SGNTTKSFLFKVNIDAAADTGITTIDAVLKGRDEVSGTSVYDSLAVSADQWTIQIPAQ
                     LTVDSVWALPATVSQNQQNIPVNVVIRNTGVATARVDSVRLRYYYGSTLSTSDFVDSL
                     TVPLGTITLVGGDSALYLFNTDALSSAASGTVRFYAVAFGQDNNSLQARVDTSRLRYD
                     SVLVQTRPVLSYTANTLTPDTVTIGDAFNYRLDISNSGEANVILTPSTQLILRDVDSL
                     KVNLQDTVFIASGYTRTLDFSTALIGLRIDSVYYPEVRFVGTENDNAFNAYLYITNDS
                     VKILSPGLVSADSFGVYDKNGALSVNISAGDTFTVRLLVRNNGGARIENLAPQPGALS
                     ISGTTIPVLLSGPTPTTAILERQDSVRFQWTYRADSSGTVNLSVRAQGADAVTDSSVQ
                     SAQRNIFLTVQNAVADTLYALTLTTDTVQVYQFTDITVVARDTANRIAQADSVRFRVL
                     TGTAGFRDTSRATIDTTIATDALGRATMRLFTGSIPSASLVRAYLRSTADDSVSFNIA
                     SVPRAVSSLQVAVNSSWTVGLNEAVTITALDQYGNVATNALSNVTLSGINTSSLQ"
     assembly_gap    5693..5792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5793..9333)
                     /locus_tag="PK937_08365"
     CDS             complement(<5793..9333)
                     /locus_tag="PK937_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32622.1"
                     /translation="MWTDSKTSTAAPYATYQWVDASAGTVITPTTGSTTGLVNIGFNF
                     VFYGNTYSQVDISSRGFLSFQNLQSNDFSTNNDLSVAGGPDSIIAAYWDAITCVTASR
                     IYYQTIGTAPHRQFVVSWNSVRDGAQNLIFQVILYETSNLIKIQYQTVGTATGATATV
                     GIDSASGTASQYSFNTANLQSTMAVLFHTNATASASATISPSSVRTNTTLNSFTYSIY
                     VNGAIDSLARIDSVLIRNPFTTKSATVTDISVDGSSKFFINSSSRPSALGLSSYDFAT
                     WSYNTTNDSISVRTNVFGIRDSIKVTFTVDAENILGNVNFPSSVLAPLVSETRLSVSG
                     SPSVSVIPDTLYTLSVKWYNQEPSGLAVDTSIIADDSLRVFAYGYDRNGNFLRRVKVN
                     WSSSGTMGFLAGATSGSDSISFHGRRVSSGYISADSAGFHDQTGLITVTVGDTSFAVL
                     RSQANNGGLRYDTLSILTTADTSMQFYLAGYDADSNYTGDLVAYFDTVYGVSSGLSGF
                     TADSSLGVVYNPTLVGSGYMRARYYNNASGYIADTTGLITVLHGDTARLVIEDARFSS
                     QAVGADTLTADQTLRLYAASYDGDGNFIGNLQSTAWSLSGGIGSLNTALSDTVILTAS
                     NAGSGSVRASYNGLSDETGLITVIGGDTSYILIRTGARNSGNVFSSALTLKADSSLVL
                     HAAGYDADNNYLGPVYVSWSLAAHTVPSLSNFTPVSDTVLTYVPALVGSDSIIAQMLG
                     DALRDTTGLITVVPGDTAMLLVETMRNGNGVSFPNLVSTADSVVTGYALYRDIKGNVI
                     DSATSGIVWSVSGRIGSIVQEGLDSAVLRLDTVGVGQLRAVAGGFSASSGFIRVSPGI
                     LYSVKLMRDTLLLGDEVDDFSISVGDSIKLYAAAFDSNGNFRGNIASSYAAHSGTLQS
                     SRLFIRSDSTSVVFWPSTASTGRIYVTSNYLGISDSSGVVTVTNTAAVAGIRINSEAH
                     NTGTEVLALTVTSDEDTTLHIASYDAFGNYLGDVAGRFSVTGSLDSLDLSPQLNTSAA
                     VYNARVAGGSGRITAEYSGFRDSTGLVSVTAGGIAFVQVQTAANDIASTLSSKSATTD
                     SNGITLYAASYDADSNFVRLVQATWSVEGGIGSVSSPGTSTSFNARRTGSGRVVADTS
                     VFEAKTGLITVTAGDTSFAVLRSQANNGGLRYDT"
     gene            complement(9432..9950)
                     /locus_tag="PK937_08370"
     CDS             complement(9432..9950)
                     /locus_tag="PK937_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32623.1"
                     /translation="MKLFFKLSALVFSFNLFLPLDLFSQSGSYVIDQTEWTEGKSAEL
                     RLAISIKNGSGKIIEIGNALNDEIQIVSIEHNTASLWMTTNQALFDKKISGTALVQIV
                     EKRAKIEFNDALKSGDKLVIKLALAVPTAHALKSHSDKNHSLDVTVSKDRNSSSVKLT
                     NSQTIKVKADAP"
     gene            complement(10194..11042)
                     /locus_tag="PK937_08375"
     CDS             complement(10194..11042)
                     /locus_tag="PK937_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNH32624.1"
                     /translation="MPKSRLPHLDETGVPLKAYDNGSFLHSPDGRTLRVISEMHEPYA
                     RFKKYGIRDTIVFYGSARILDPKDAKRKLLVLQKEANNQLKPSVSLRRQIERVQSDLE
                     MSAYYRDAMKLAEMLTRWSSKLDGGKRFIVCSGGGPGIMEAANRGAKKAGGPTIGLNI
                     SLPFEQIPNPYISPELNFEFHYFFMRKFWFAYLAKALVVFPGGYGTLDELFELLTLVQ
                     TKKIEKPLAILIYGKKYWDEIIDFEAMAKHHMISPEDLQLFSYADKPEEAFEYLREKL
                     ERYYLK"
     gene            complement(11085..14147)
                     /locus_tag="PK937_08380"
     CDS             complement(11085..14147)
                     /locus_tag="PK937_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH32625.1"
                     /translation="MKNIIMILLYAFLGTYICAQNQSTFSPFQELSEPPVLTFESGLH
                     LDRVNAVRYSPDGKEIVTASWDKTIRIWDAWTGQLLRSLRVPGYAGTEGQIFTMDISP
                     DKKYILIAGSSVGQRYNTLTANYIGDYVLMLDYVSGAVVDVAAHHKQSIYGVRFSPDG
                     KKVVSVGGSFDNRINQYDVNTNTGHLVLRDSAVTTQEAVKAGLCNWGFLGICDHQVVG
                     TGFVDNGNAWLAADYHGLVFRKKAGENLRVIGKAVDGPCRSIATDAKGKLAAIGDFRG
                     RVRIIDALQNEGDNIVANIMTFKSDMITAMVFDTAGEQLAVCVGNEVRVYSLTSEADG
                     RLSKKHVISFRQHDRPVFAVAFSPDGKHVVSAGGLHNEIHVWNVADGERIMTLGDVSK
                     SGKISAIGIHRDHPNVIGIGETFNFYEKLNDYGTISKTFNLSTFEFKTDSVEQSLFEN
                     ARLAQLRAPVSLPDIKWLLDDRPLCAMKLQNGKLLLGTYYGIFIDSPANMLSYDGART
                     IGLAESPDGRRFYVGLESGEIKIYDENKMTYIASLYVTSDNEWIIWTTDGYYAASKRG
                     AQRVGWQINKGLNMTPKFYPFEQFDLRLNRPDIVLQRLGVADSARIAALYGAYQRRLK
                     RLGLTESQLGSSLQVPDIQIKGDVTETDLREVILTISAYSKEAPLERLMVNVNDVPLH
                     SSYGKKIGDGYLNQWNETQRIPLTQGRNKIQVSVINTAGIESLKETRYVHCRSQDTDP
                     ALYVIAVGVSHYTDREFDLQFAAKDAGDITDFFRNALASNKLPYSKLHIKTLLNEQAT
                     KDSIIQVRNFIANCKPQDGVVMYFAGHGIPDSTVDWVFATYDIDFYKASEKGITFAEM
                     EGLLDGIAARKKLMLVDACHSGERDRDETEIVDNAKDTKAQQVRAFKNVKTKSKSGVG
                     LVNSFELMQHLFSDLQRGSGAVVISAASGTEWAAEGDIGGKTIKNGVFTYGLLEGLRT
                     MAADRDKNGKITVTELRDYVMQSVRTLTRGAQHPTARKENLEFDFRVY"
     gene            complement(14217..15974)
                     /locus_tag="PK937_08385"
     CDS             complement(14217..15974)
                     /locus_tag="PK937_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32626.1"
                     /translation="MFRSLFIALFLSVTLQAQLKIAPGMPMYNAPADSLCEYGRAGVE
                     MKSEMMALTYYNHAIDKYPQHPRAYAEVAMMYAEFGKFEVAKEYLQQAESIDNKYPMT
                     LFARFTFIREEARSKYKFDQMPKDVASAVVEAGRAFLAVKDTSMNDRKAYIKQFLPTY
                     QLVSDDIEAFKMYRLALQDKENNNFEDAFKKFETIITRVQNTGNTGIISSVSTEAGIC
                     KFKLGEYPAAKTWFQKSIATPEAEGLAYKFMAQILYQIDKNVVEAEKLCLSGLSKNLS
                     GSDEIKTYLCNIYFTDGKDAFDKKNYWAAATNLDKLVKYDDKNAQAYGYLLYALYQTK
                     QYTKAGIAVQKLLELNEAEENLALYPNLTDIQKFCKNPVGAAPNFKYNQIVADSADLK
                     IKTLDLMYQLKMYDKAVEESQNALAMYSRIGHKAGMAWAYYKLGMAYHYKSDVANAKL
                     NYQKSIDTKPITGGVYSNLYQIINSENPAEAHKILDSGYKLFPDHDRLKGLMSDFYWN
                     KAWEEMSAQNYNNAIDLSNKSNEMSENAVAYFVLGYSYYALGKGSDALYCMQRAKQLD
                     PEMPKQYPQIDQIINYYSK"
     gene            complement(16170..17960)
                     /locus_tag="PK937_08390"
     CDS             complement(16170..17960)
                     /locus_tag="PK937_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH32627.1"
                     /translation="MFKRLKPYLWKYRVYWISGFVLVILKNLFQSTGPQLVREAIDTL
                     APGTDPSTFPLYNQIIHSVELQLGVAIVVAYIAAYLLVELLHGIFLYLMRQTLIVMSR
                     KIEYDLRSDFFSHLQKLHVQFFQYAKTGDLMSRLTNDLANVREVLGPGIMYTANTIVA
                     FIYVVPMMIHISPMLTLLAFLPLFLLSFAVGRLSGVIMTRSQKVQEKLSDISSAAQEN
                     FSGIRVVKSFVQEAAEIKKFENLSHEYIQLNMSMVRIRGIMMSSVILTIGLSVAVLLW
                     YGGSLVIDGVMSIGEFTAFNFYLAMLIWPMVAVGWVINIFQRGSASMKRMNAIFDEQP
                     AISDKNNQSNGHSDIAGKIEFRNLNFSYRDIPVLKNISICIHPGMTLGIVGATGSGKS
                     TLVNLIPRLYDIPDGMLFIDDRDINTIPVSILRQHIGMVTQDNFLFSDTIYQNIVFGV
                     DNPPTDKVIWAVDTAQLRSNIEDFPEKYETIIGERGITLSGGQKQRLSIARAVIREPK
                     ILILDDALSSVDTHTEDEILKRLKILMKNRTTLLVSHRIQTVKHADLIIVLKEGAIIE
                     TGTHDDLVEQNGTYAAMHERQLLEEEVANI"
     gene            complement(17967..18554)
                     /gene="nadD"
                     /locus_tag="PK937_08395"
     CDS             complement(17967..18554)
                     /gene="nadD"
                     /locus_tag="PK937_08395"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNH32628.1"
                     /translation="MEKIGLFGGTFNPIHAGHLIIAEIIRDHLGLSKVVFIPSARPPH
                     KTSENVISFHHRKTMVDLAIEQNEYFQCSDIEEKLSGLSYTVHTLEFLRKENPKNEYF
                     LMIGSDSLLDLPNWKTPERLTELSKIVVYPRMGFDFDKGELQFTKEAICIDKPVIEIA
                     SSWVRERLLRNQSVRYIVPNAVLSYIKKNNLYTKE"
     gene            complement(18564..19400)
                     /gene="bamD"
                     /locus_tag="PK937_08400"
     CDS             complement(18564..19400)
                     /gene="bamD"
                     /locus_tag="PK937_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNH32629.1"
                     /translation="MLRKIFVLAMACSFLQSTGCSKKDVTQYMTSREHFEYAMKFFNK
                     KNWTSAQEQFSLITYKYSGSDIADDAQFYLAESYYRQKDWVSASSEFERMVSSYPKSE
                     FIEKSMYQLALCYIELSPGFALDQKFTYQAQNAIQNFIDLFPRSELRTEMDKHYKSVK
                     DKLARKHFESAQIYRKISEFEAAIVYYDQVITDYYDSPYAMQAKYWKGYCNYKIEEFQ
                     KAQILLKKFIDEYPLEKEWVTDALETLEDIKKDELKAQAKAAKEAKLVKPEQSTSVKS
                     AN"
     gene            complement(19453..20226)
                     /locus_tag="PK937_08405"
     CDS             complement(19453..20226)
                     /locus_tag="PK937_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNH32630.1"
                     /translation="MCRLMGYAGPNPVSLQNVILDAPNGILRQSVCDRDSCKPNDDGW
                     GFGFEENGQFHVIKEASAPRNDKLFLDTAHRPFKRVMLHIRRASVGGASKVNTHPWIY
                     HQDIIFAHNGSVYGIENVMDSALAKIDPALSGHITGNTDSEYAMHLFLTYLRKYNPAQ
                     TLDIHFVRTSLQHTIADLREISRRADASRKPQLNFMMLTRDYLIATKVMHSLGYYTSY
                     QGGTLVTSEALDNNDGWTLMKEETMIVVSRNGDYALYDI"
     gene            complement(20226..>22092)
                     /locus_tag="PK937_08410"
     CDS             complement(20226..>22092)
                     /locus_tag="PK937_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32631.1"
                     /translation="AEYNYPNDTLEGIIINRLGQSNNIKRQFRILDHIIETTLSGDLN
                     IELGSKTEKLEWLQLINSLEINVALAQYEEKLAQDNFPTLDDEIKEEIRRVLAADNIA
                     DQTIAAISFARRLDNPDESLHDPAKLVEIAAETQYLTSMIFKIKGFQIKEVYEFVELI
                     GELDAKKDDYRRVVEYFKYELPHIDKGVMKEEDSDIKKAALAARYLSARLMSKFDIDP
                     SYLKLSTDYIGLDLTKMPPDMTVDEDELNQRISHFHEIRKRLGFFNSAERAFNAINQV
                     ADFIDKERLTLDKPGVHRILDQINDAELGLAVADYVRVGTITKGSDDGDAFNVKYVEA
                     KVKAVFKEESIRAIESDAVKKNIIPESVLAKAEEEGTFEETLLDIKNYGTYLMTRRLD
                     RPEYPRHTQRAVNLILALLQDAIGWRHFENTQIRAKQEVVPLIDEYHRFLGKLLDALD
                     DDKNAEHEEVIGKLRDDDYFAVNDNKDSIEAGKKSAPQVKAEFMTLMNDERFQNYVSQ
                     AMGYAETSTHKDWIIYKSALETIKSDLNSHIGKTVTVIEGVVQQLSGDSKNADRFRPF
                     IETLQGKYLDSFKRMQYQLDQNKKPATILKIEYDRLANSEKFKVYLKHCFELIKH"
BASE COUNT         6276 a         5213 c         4359 g         6144 t
ORIGIN      
        1 tctgtagcga agacgtattg ataccggaca aggttacatt cgacaatgca ttcgtcgcta
       61 cattaccata ttgatccagc gccgtaatcg tcaccgcctc attcaacccc accgtccatg
      121 aagagttaac agctacctgt aacgatgata cagcacgagg gacggatgca atattaaaag
      181 aaacactgtc atcagcggtc gaacgcaaat aagcccgcac caatgaagct gaaggaattg
      241 atccagtaaa caaccgcatc gtggctcggc ccaatgcgtc tgtagcaatg gtcgtatcta
      301 tcgtagcgcg actcgtgtca cggaatcccg ccgtgcctgt caaaacgcgg aaacggacag
      361 aatccgcttg cgctatccta tttgccgtat ctctggcaac aacggtaata tcagtaaact
      421 gataaacttg aaccgtatca gtggtcaacg tcaatgcata caatgtatcg gctacagcat
      481 tttgcactgt aaggaatata ttacgctgtg ccgattgcac ggacgaatcg gtaacagcat
      541 ccgcaccttg tgcacgtacg gaaaggttga ctgtaccact actatcagcc cgataagtcc
      601 attggaaacg tacagaatcc tgacgttcta aaattgccgt agtcggtgtc ggaccgctta
      661 acaatacagg tatagttgta ccgctgattg ataaagcacc gggttgcggc gcaaggtttt
      721 ctatacgcgc accaccgtta ttacgaacta ataaccggac cgtaaacgta tcgccggctg
      781 aaatattgac agacaaagcg ccattcttat cgtaaactcc aaacgagtct gccgatacca
      841 acccaggact caatattttt acgctatcat tggtaatata caggtacgcg ttaaatgcat
      901 tatcattttc ggtgccaaca aaacgaactt cgggataata cacgctgtca atgcggagcc
      961 cgatcaatgc cgtcgaaaaa tcaagcgtgc gtgtatagcc ggatgcgata aatacagtat
     1021 cttgtaaatt gaccttgagg gaatccacat cacgcaaaat gagctgtgta gacggcgtaa
     1081 gtattacatt ggcttcaccg ctattggaaa tgtctaatct ataattaaac gcatcaccga
     1141 tcgtaaccgt atctggtgtt aaagtatttg cggtatacga gagaacgggt ctggtttgaa
     1201 ccaatacgct atcataacgt aaacgtgatg tgtccacgcg cgcttgtaga ctgttgttgt
     1261 cttgaccgaa agcaacagca taaaaacgaa ccgtacctga agcggcagac gacaaagcgt
     1321 ctgtattgaa cagatataaa gcgctatctc cgcctacaag agttatcgtc ccaagaggga
     1381 cggtaagcga atcaacaaaa tccgaagtcg acaaagtact tccatagtaa taccgcaatc
     1441 gaacactatc aactcgggcc gtcgcaacac ctgtatttcg tataaccaca ttaacaggta
     1501 tgttttgctg attttgagaa accgtcgccg gtaatgccca gacactatca acagttaatt
     1561 gtgccggtat ctgtatcgtc cattgatcag ccgaaacggc aagcgaatca tatactgatg
     1621 taccggaaac ttcatccctt cccttcaata cagcgtcaat cgtcgttatt ccggtatcag
     1681 ctgccgcatc aatattaact ttgaatagga atgactttgt cgtattaccg gataccgtat
     1741 cgctgagatc gctatatggc gttacgtcaa acccggtatt aagcaacgat accgcttcat
     1801 caaaaaattt caattgaatg gtgtcaattc gtccaacgga tgaactaaga ttagtcaccg
     1861 aaacacgtac aaggatacta tcctgtccac ggcttactaa ggtcggcgta gtctgtacgc
     1921 tattcaatgc gatcagcgaa ttgcttagta tcagcaatgt gtctcgaccc gtagatgtaa
     1981 ggctgatcat tctttgattg agttgatccg aacccgatac atatgcgccg atcgaatcca
     2041 atcctgttgc ggaattggat aaaacatgtg ctgtaaaatt atatgcccgt gtttcgtaac
     2101 catacaatgt atccggcaga gtttgcgtta atgttgtatc atataaacct cgcttgaata
     2161 caagctgaag gtcagatatt gtagccggcg atccgccttg gttggctatc gtcaccacaa
     2221 agggtatgtt gttctgaccg acagcaactg tatcgtgcga aggactgact accgatataa
     2281 ttgaaagcgt tgcacgttta cgaacaaata tagagtcttt atctgttacc ggtgacagat
     2341 taactgtatt tccgctgttg atatcatgcg ctacaatacg cccgtcaaat acgatacttc
     2401 cggtgtccgc cacagacgaa atgagagcat atagcgtaaa gacgcttgag ctgttacctg
     2461 cgatagtatc cggtagacta ccggtaccaa ctacagagta tccgctgata gtagctaaat
     2521 ttcgagtagc gtctaagatg atagaatcaa taacagccga agcctgtgca ttattgacga
     2581 tgcgaacacg taatgcaacg gaatcgcggt attgcgcggt ggtatccaat gcgatctcaa
     2641 cgctgtcaaa ttcaagactg gctgtcgtta cggttgtttg cacagtaaga ctgtctgcac
     2701 ccgtaaataa tgtcgtatca aataatccgc cattctccgt accattcaac accaatacac
     2761 cctgatatct accggatgtc gcgttggtga tcgtcaacgg atcaaaaagc agcgttgtag
     2821 atgctccgga accgggaact acttgagatg atgtcagata ggtactatcg ccgccaaagc
     2881 gcagataggt atttgcttta tctaagataa cagacgccag tgaatcattg ataaccgata
     2941 tccgaaatgc gacagactga ccttctgaaa cggtatccgg cgacagggtc gctgccacat
     3001 aacgaagact cgcatcagat tgaatgatga tggtatccat cgtattggct aacgtatcgg
     3061 aaacgcccac gccgccgttt gaaccggagt aaaatccgtt cacaactatc tgtccgagcg
     3121 gcgccgatgc tctaactacg gcggtgatat tgaatacact ggtagaacgt ggtgcaaacg
     3181 gagtggcatc aatcgagctg atcaaatcaa aaactcccga cttgctcgtc aatagcccgt
     3241 agttcagatt tgtaagtgta acattgctcg aattataaac ctgtagttga actgttatag
     3301 tatcgccacg gccatacacc ttcggtgctg gggtattaac agatatcacg tcaacaccga
     3361 ggatattcaa aacatttgca ccggtttcga gcgtcccata tagtgtgtca ctgacggacg
     3421 ccacctgacc gcgcaaatca aaacgtacat taaattgttt tccaccgaag gaactcggaa
     3481 tggtatctgt tctgaattgc aacgtatctt gaccgtgagc acgaattacg gtatctccat
     3541 acgacaataa cgaacggaaa gtagtcacac catcagagaa acgaacgcgc gtttggtttg
     3601 aatccaaagc aacaggatgt ggtccataat ttataaatgc cgccgtaaaa gttgcttccg
     3661 tatcggcgag cgcaatcgta ggcttgagcg atccgacaac ataacgcaag gcgtatgttg
     3721 taaaaatagg tcctccgacc aaaccggacg ttgttgcttg aacagtagcg ctatcaccgc
     3781 cgccaagctg caattcaatt aacgagatac cctgtgcatt cgacatggcc gagtcgccac
     3841 cgacaaaaat caccgaaccg gcaagtacat tttggcgaac atatttaccg ctgataacat
     3901 taccaaactg atcttcaagg cgtgttttaa gtgtgaccaa accgccaagc ggtacaaccg
     3961 ttgcagaatc gccttccgtt ttgattatgg caaaaggtgc atcgggcacc gtcgtagctg
     4021 tgaataatac ggtatctatt ccgggaataa aggcgcgtac actatcggac cctgtaagat
     4081 cttttaatcg ccagttcgct tttgcaatac cttgcgtaaa tgccaatgcc gaatctcgga
     4141 acgtgggtgt tcctgaaacg gaatctatag atacggcatc taccaggcct tgattcaaaa
     4201 cgctgacgga attatttatg gcctcccaaa ttacgcgtgc cgtatcgttg actacatttc
     4261 cgtaacgatc atatacctga acacgtaatg tgtcaacaat ttgatctacc gtatccgttt
     4321 gtgcattacc tgaaagtaaa cgaagttctc ccggtaaacc aaacgtcgct tgtatggtat
     4381 caaatactgc gggattgagc agagacgggt tggatgcacg gattatatat tgaccaactt
     4441 tcgtgcccat atgaaatata gccgaagcat agccgttgat atccgtaacc gtatctaatg
     4501 tagtaacata gtttcccgca ccgcggataa atcctgtact gtccgatccg gatggatacg
     4561 aatacgccgt gaatgaaatc gccgtattgg acaccggatt gctaaattga tctcttacta
     4621 atacgcggaa cgtatccggt agcatttggt tgacggtatc ggaatttacg agtgagctga
     4681 cacgaattaa tgtatcttcg ccagtcggaa gcagattggc tataaattgc aatgagtcgc
     4741 ccgttacagg tatcactgca tacatcgtat cgtacgatga cgcgccggtg cgcaatgtcc
     4801 aaagtgaacg cgcaataccg tcattatccg tcgtgcgaat aacgggtgat gccgtgccgt
     4861 cggatgttgc aaaccattgt accgttacgg tatcattgac ggtgttgcca aactgatctt
     4921 taacaactaa tgccaaagag tccagaagtg gttgaccaaa ttgaccggat tgtacattag
     4981 ttaagctgtc aggataaaac tccactatag acggcgctgc ggctcgcagt gtgataagat
     5041 tggttgccgt tcgtttagtt gtatccagta aactcgtaac gcgtatttgt aaggtttctt
     5101 tgaccgaatc cgtcacgacg actgtcaact gtccgttggt tagcgtgtcg tttgtaggat
     5161 tgatccccac gccgctgcca ttcagccctg ttacactacc gataacatca aactgcacgc
     5221 gcgtggtatc gtcatctaat gtattattga atatatcgta cgccgtgact gtcgcggtta
     5281 ttgcaacacc caccgtgtca ttattggttg aaggtatgat cgtataatac gccaatacag
     5341 ccgggtttgt ggttatcgta aagcgacccg aatcggtgct aagacttgtt atgtatgcac
     5401 gcactgtgga cacaccgctt gttgtaggcg ctgtaaacac aacctgcgat acgcctaatt
     5461 catctgtaga atcactggct aaactgagtg ctccacttcc cgtttggatg gtccaataga
     5521 cgacactgtc ttgcaatcca tttccatcat catctgtcag tttaactttt aacgttgtcg
     5581 tctgtgtcgg agtcaaagat acagaggtag atgatatttc ataaatcgaa tccggtgcgc
     5641 cccctataac cgtgatcaag cccgtctcat cgctaagacc attgtaactt gcnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgtatca taccgcaaac ccccgttatt
     5821 cgcctgactg cgaagcaccg caaacgacgt gtcgccggcc gtgaccgtaa tcaatcctgt
     5881 cttcgcctca aataccgacg tgtccgctac cacccggcct gaacccgtgc ggcgcgcatt
     5941 aaaactcgta ctcgtacccg ggctactcac actccctata ccaccctcaa cactccaagt
     6001 ggcctgcacc aaacgtacaa aattactatc cgcatcatag gaggccgcat acagcgtgat
     6061 cccgttactg tccgtcgtcg cactcttgga ggagagcgta ctggctatat cattcgccgc
     6121 cgtctgtacc tgcacaaatg caatcccgcc cgctgtcaca ctcaccaagc cggtgctgtc
     6181 tctaaaaccg ctgtattccg ccgtaatccg gccgctaccg cccgccacac gcgcattata
     6241 taccgcggcc gaggtattca actgcggcga taaatccaaa ctatccaaac tgcccgttac
     6301 actaaaacgc cccgccacat cacccaaata attaccaaac gcatcatacg atgcaatatg
     6361 aagcgtcgta tcctcatcgc tcgtcacggt caatgccaac acctccgtgc ccgtgttatg
     6421 cgcttcacta ttaatccgga taccggccac cgccgccgta ttggtcaccg taaccactcc
     6481 gctgctgtcg ctaataccca aataattact cgtaacataa atacggcccg tagatgccgt
     6541 actcggccaa aataccacac tcgtactgtc actgcgtatg aaaagacgcg aggactgaag
     6601 cgtaccgcta tgcgccgcat acgaagacgc tatattaccc ctaaaattac cattactgtc
     6661 aaacgccgcc gcatataact taatactatc cccgacactg atactaaaat catccacctc
     6721 atcccccaaa agcagcgtat cacgcatcag cttcacactg taaagtatac ccggactcac
     6781 gcgtataaat ccgctcgaag cgctaaatcc gcccgctacc gcccttaact gacccacacc
     6841 caccgtgtcc aatcgcaata ccgcgctgtc caatccctcc tgcacgatac tccctatacg
     6901 gcccgataca ctccatacaa taccgctcgt cgcactatct atcacattac ccttgatatc
     6961 acgatacagc gcataacctg tcaccacact atcggccgta ctcaccaaat tcggaaaact
     7021 tacgccattc ccattacgca ttgtctccac caaaagcatc gccgtatcac cgggaaccac
     7081 cgtaatcagt cccgtcgtat cccgaagtgc atcacccagc atctgcgcta taatactgtc
     7141 actacccacc agcgcaggta catacgtcag caccgtatca ctaacaggcg taaaattcga
     7201 taaactcggc accgtatgag cggccagtga ccaactcaca tacacaggac ctaaataatt
     7261 attatccgca tcataacccg ccgcatgaag cacaaggctg ctatcggcct taagcgtcaa
     7321 cgcactcgaa aatacattcc cgctattcct tgctcccgta cgtatcaata tataactcgt
     7381 atcgccccct ataaccgtga tcaagcccgt ctcatcgcta agaccattgt aacttgcacg
     7441 cacactccct gaacccgcat tagaggccgt cagaatcacc gtatcggata acgccgtatt
     7501 caaactccct ataccaccgc tcaaactcca agccgtgctc tgcaaattac ctataaaatt
     7561 accatccccg tcataggaag ccgcatacaa acgtaatgtc tgatccgccg ttaatgtatc
     7621 cgcccctacc gcctggctgc taaagcgggc atcctctatc acaagacgcg ccgtatcccc
     7681 atgcaatacc gtaataagtc ccgtcgtatc cgctatataa ccggatgcat tattgtaata
     7741 acgcgcacgc atataaccag aacccaccag cgtcggatta tacaccacgc ccaaagaaga
     7801 atccgccgta aaaccgctta atccgcttga aacaccatac accgtatcaa aatacgctac
     7861 caagtcaccc gtataattac tatccgcatc ataacccgct aaataaaact gcatgctcgt
     7921 atccgctgtc gtcaaaatac tcagcgtatc ataccgcaaa cccccgttat tcgcctgact
     7981 gcgaagcacc gcaaacgaag tgtcaccgac ggtaaccgta atgagaccgg tttgatcatg
     8041 aaaaccggcc gaatcagcag aaatataacc actgcttaca cggcggccat gaaacgaaat
     8101 gctatcggat ccgcttgtcg ctccggccaa aaaacccatc gtacctgaac tcgaccaatt
     8161 gactttaaca cgacgcagaa aatttccatt ccgatcataa ccatatgcaa agacacgtag
     8221 tgaatcatct gctatgattg aggtatcaac ggcaagaccg gatggttctt gattgtacca
     8281 tttgacagat aaagtgtaca gtgtatcagg aataactgaa accgaaggac tcccgctgac
     8341 cgataatcgt gtctcgctga ccaaaggcgc aagtacagat gaagggaagt tgacattgcc
     8401 taaaatattt tcagcatcga cagtaaatgt aactttgata gagtctctga ttccaaacac
     8461 attggtacga acgctgatcg aatcattcgt tgtattataa ctccatgtgg cgaagtcata
     8521 cgatgaaaga ccaagcgccg atggtcgcga agaagaattg ataaaaaatt tcgatgaacc
     8581 gtcaacggat atatctgtta ccgtggccga tttagttgta aatggattac gtattaacac
     8641 ggagtctatt cgcgcaaggg aatctatcgc cccattcaca taaatcgaat atgtaaacga
     8701 atttagtgtt gtgtttgttc taacgcttga cggagaaatc gtagcactgg cggacgccgt
     8761 ggcattggta tgaaacagta ccgccatcgt actttgtaaa ttggccgtat taaaactata
     8821 ttgtgatgcg gtaccactag cactatcaat tccaacagtg gctgtcgctc cggtagcggt
     8881 tcccactgtt tgatactgta tcttgatcag attggaagtc tcatacagga tcacttggaa
     8941 tattaaattt tgcgcaccat cccttacgct attccatgag acaacaaatt gtcgatgggg
     9001 cgctgttccg atcgtctgat aatatatacg tgaagctgtc acgcatgtga tagcatccca
     9061 atatgctgca ataatgctat ccggcccacc agctactgaa agatcattgt ttgtagaaaa
     9121 atcgttggat tgaagatttt gaaaactgag aaatccgcgt gaactaatat ctacttgaga
     9181 gtaggtatta ccgtaaaaaa caaaattgaa tccgatattt accaaccctg ttgttgagcc
     9241 ggtcgtggga gtgattacag taccggctga tgcatcaacc cattgatatg ttgcataagg
     9301 tgcagcggtt gaggtcttgc tgtccgtcca catgtaggca cggtttgaac caccctgtcc
     9361 cgcagataca acaaacgaga aaaaaaggaa taataaaaaa ctatatttgg ttgcgcgtat
     9421 caaggaatgc atcaaggtgc gtcagcttta actttaattg tttgcgaatt ggttaatttc
     9481 acagaactgg aattacgatc tttacttacg gtaacatcta aactatgatt tttatcagaa
     9541 tgtgatttta acgcatgagc ggtgggcaca gccaatgcca atttgatgac aagcttatca
     9601 ccggatttta aagcgtcatt aaattcaatc ttagcacgtt tttcaacaat ttgaaccaat
     9661 gccgtacccg aaattttttt atcaaataat gcttggtttg tggtcatcca tagtgaagca
     9721 gtattgtgtt caatggaaac gatctgaatt tcatcattca acgcattccc aatctctatg
     9781 atcttaccac ttccgttctt tatggaaatg gccaatctca attcggcgct ctttccctct
     9841 gtccattcgg tttgatcaat aacataactt ccgctttggg aaaacaaatc aagcggcaaa
     9901 aataaattaa agctaaatac cagagccgaa agtttaaaaa aaagtttcat tcttctctca
     9961 tactgaagaa agcgattaga aaaaattaac acaccgaaga cttgatttac tgaaatgaat
    10021 tagcaaatca aacaaaaagt gacgaaccga tacacataaa aaaataatag caaaaacacg
    10081 ccgtctttgc aaacaaattc aataaaatga taatgataga taaaaactaa gccgttgaga
    10141 tatatcacaa cggcctataa aaaatactta gatataaaac taatccaatt tctttatttt
    10201 aaataataac gctccagttt ttctcttaag tattcaaagg cttcttcagg tttgtcagca
    10261 taagaaaaaa gctgtaagtc ctcaggactt atcatgtgat gcttggccat agcctcgaaa
    10321 tctataatct catcccaata ttttttaccg taaataagta tagctaatgg tttttctatc
    10381 tttttggttt gaactaaagt cagtaattcg aataattcgt ctaaagtacc ataaccaccg
    10441 ggaaaaacga caagcgcttt agcgaggtaa gcaaaccaga attttctcat aaaaaaataa
    10501 tgaaactcaa aatttaactc tggcgaaata tagggattgg gaatttgttc aaagggcaag
    10561 cttatgttga gcccgatcgt cgggcctccg gcctttttgg ctccgcgatt cgccgcctcc
    10621 ataatgcctg ggccgccgcc gctgcatacg ataaagcgtt tacctccgtc cagtttggat
    10681 gaccaccttg tcagcatttc agcaagcttc atagcatcac ggtaatatgc ggacatttct
    10741 aaatctgact gaactcgttc gatttggcgt cgtaacgata ccgacggttt taattgatta
    10801 ttcgcttctt tctgtaaaac cagcaatttg cgtttggcat ctttgggatc aagaatgcga
    10861 gcggatccgt aaaatacgat cgtatcacgt atcccatact tcttgaagcg cgcatacggt
    10921 tcatgcattt cactaataac acgcaaagtt cgaccgtcag ggctatgcaa aaaagaacca
    10981 ttgtcatatg cttttagagg cacacctgtt tcatccaaat gcggcaaccg agatttgggc
    11041 ataatacgtt ccttaggata acttataaat aggacggtta taacttaata cacacgaaag
    11101 tcaaactcca aattttcttt tcgtgccgtc ggatgttgag cgccacgcgt caaagtacgg
    11161 acactttgca tcacgtaatc cctcaattcg gtcacggtta tttttccgtt cttatcccga
    11221 tccgcggcca ttgtacgcag accttctaaa aggccataag taaatactcc gttttttatc
    11281 gtcttaccac cgatatcgcc ttcagccgcc cactccgtac ccgatgcagc cgaaatcaca
    11341 acagcgccag aacctcgctg taaatccgaa aaaagatgct gcattaattc aaaagaatta
    11401 accaaaccta caccggattt tgatttggtt ttaacgtttt taaatgcgcg cacttgttgt
    11461 gctttggtat ctttggcgtt atctacgatt tctgtttcat cccgatcacg ttcacccgaa
    11521 tgacatgcat ctacaagcat caatttttta cgagcagcaa tgccatcgag caagccttcc
    11581 atctcagcga atgtgattcc cttttcggaa gctttgtaaa agtcaatatc gtatgtagcg
    11641 aagacccaat ctaccgtgct atccggtatt ccatgacctg caaaatacat tacaacgcca
    11701 tcctgaggtt tacaatttgc tataaaattt cgcacttgga tgatgctgtc ttttgtggct
    11761 tgttcgttaa gtagcgtctt gatgtgtaat tttgaatatg gcaatttatt tgaagctaat
    11821 gcgtttcgaa aaaaatctgt tatgtccccc gcatctttag ccgcaaattg taagtcaaat
    11881 tcacgatcgg tatagtgtga tactcccact gcaataacgt acaatgcagg atctgtgtcc
    11941 tgtgatctac agtgcacata gcgcgtctct tttaggcttt cgatgccggc ggtattgatc
    12001 acggacacct gtattttgtt acgcccctga gtaagaggta tccgctgtgt ctcgttccat
    12061 tggttaagat aaccgtcacc gatttttttc ccataagatg aatgcaaagg aacgtcattc
    12121 acatttacca ttagacgttc aagtggcgct tctttgctgt aggcggaaat cgtgagtatt
    12181 acttcgcgta aatccgtttc cgttacatcc ccttttatct gaatatcagg aacctgaaga
    12241 gaagagccga gttggctttc ggtaagccct aaccgtttaa ggcgccgctg atacgcacca
    12301 tacagtgctg cgattcgagc tgaatcggct acaccaagtc tttgtaatac gatatccgga
    12361 cgattaagcc tcagatcaaa ttgctcgaat gggtagaatt ttggcgtcat atttagacct
    12421 ttattgatct gccagcccac tctttgtgct ccgcgtttgg atgcagcata gtatccatcc
    12481 gttgtccaga ttatccattc gttatcggaa gttacataca gcgaagcgat atatgtcatt
    12541 ttgttttcat catatatttt gatttcaccg ctctcaagac cgacataaaa ccggcgccca
    12601 tccgggcttt cagccagtcc tatcgtgcgc gcaccgtcat agctaagcat attggccggt
    12661 gaatcaatga aaattccata atacgtaccc aaaagtagtt ttccgttctg cagcttcatg
    12721 gcacacagtg gacgatcgtc cagcaaccat ttgatatcgg gaagcgatac cggcgctctc
    12781 aattgcgcaa gccttgcgtt ttcaaataag ctctgttcaa cactatccgt tttaaattca
    12841 aacgtagaga gattaaaagt tttagaaatt gtaccatagt cattcaattt ttcataaaag
    12901 ttaaatgttt caccgatccc gatgacattg ggatgatcac gatgaatacc tatagcgctt
    12961 atctttcctg acttcgatac atccccaagc gtcattattc tttccccatc ggcgacattc
    13021 caaacatgga tttcgttatg caatccaccg gcggacacta cgtgtttccc atccgggcta
    13081 aatgctacag caaacacggg acggtcatgc tgtcgaaatg aaataacatg ttttttagat
    13141 aatctgccgt ccgcctctga ggtaagtgaa taaacgcgca cttcattacc cacacagaca
    13201 gcgagttgtt ctcccgctgt atcaaacacc atcgcagtga tcatatccga tttgaaggtc
    13261 atgatattag caacaatgtt atcgccctcg ttttgcagtg catcaataat acgcacgcgc
    13321 cctctaaaat ctccgatagc ggcaagtttt cctttagcat cggtagcaat acttcggcac
    13381 ggcccatcta cggctttccc gatgacacgt aaattttctc cagctttttt tctgaaaacc
    13441 aacccatgat aatccgctgc caaccatgca tttccgttat ccacaaaacc tgttcctacc
    13501 acctgatggt cgcagatgcc tagaaagccc caattacaaa ggccggcttt tacggcttcc
    13561 tgcgtagtta cggccgaatc acggagcaca aggtgtcccg tgttagtatt cacatcatat
    13621 tggttaatgc gattatcaaa cgaaccgccc accgaaacaa ccttttttcc gtccggagaa
    13681 aaacgaaccc cataaataga ttgtttatga tgtgcagcga catcaacgac agcaccgctc
    13741 acataatcaa gcatcagcac ataatcgccg atgtaattgg ccgtcaaagt attatatctt
    13801 tgacctacgg aagagcctgc aatgaggatg tactttttat ccggagaaat gtccatggta
    13861 aatatctgcc cttccgtacc ggcatatcct ggaacgcgca agcttcgtaa aagctgccct
    13921 gtccatgcat cccaaattcg tatggtttta tcccatgaag ctgtaacaat ctctttacca
    13981 tccgggctat agcgtacagc attaacacga tcaagatgca atccgctttc aaaggttagc
    14041 accggtggtt ctgataattc ttggaacggc gaaaaagtgg attgattttg agcacatata
    14101 tatgtgccta aaaatgcata caataaaatc attattatat ttttcatatc ttactcaata
    14161 aaaaaagcgg ctatcgcaat cagcaacaac cgctttccta ggttggacac aggttcttat
    14221 ttagaataat aattaataat ctgatctatc tgaggatact gtttgggcat ttccgggtcg
    14281 agttgcttgg cgcgctgcat acagtacaag gcatcacttc ctttgcctaa ggcatagtat
    14341 gaatatccca gaacaaaata agcgacggcg ttttcactca tctcatttga tttattgctg
    14401 agatcaattg cattgttgta attttgagcg ctcatttcct cccaagcctt attccaataa
    14461 aaatcggaca tgagaccttt gagtctgtca tgatcgggaa ataatttata tcctgaatct
    14521 aatattttat gagcttctgc agggttttcg ctatttatta tttgatatag attgctatat
    14581 acgccgccag taatcggctt agtatcgatc gatttctggt aattgagctt ggcattggct
    14641 acatcgcttt tatagtgata ggccattcca agtttataat acgcccatgc cattccggct
    14701 ttgtgcccga tgcgggagta cattgccaat gcattttgac tttcctcaac ggctttatca
    14761 tacattttta attgatacat caaatccagc gttttaattt ttaaatcggc agaatcggct
    14821 acgatttggt tatacttaaa attaggtgcc gcacctacgg ggtttttgca aaacttttga
    14881 atgtctgtca gattaggata cagcgctaaa ttttcttcag cttcgttaag ttctaatagt
    14941 ttttgtacgg caatgccggc cttggtatac tgctttgtct ggtaaagtgc atagagtaaa
    15001 tatccatacg cttgagcgtt tttatcatcg tacttcacca gtttatcaag attcgttgca
    15061 gcggcccaat agttcttttt atcaaatgcg tctttgccgt cggtaaaata tatattacat
    15121 aagtatgttt tgatctcatc cgatccggac aaatttttag ataatcccga taaacacagt
    15181 ttctccgctt cgactacgtt cttatctatt tggtacagta tctgagccat aaatttataa
    15241 gcaagtcctt cggcttcagg tgttgctata gatttttgaa accatgtttt tgcggcagga
    15301 tattcaccta atttgaattt acaaatccca gcttccgtac taacagatga aatgattccg
    15361 gtatttcctg tgttttgaac acgagttata atggtttcaa attttttaaa ggcatcctca
    15421 aagttgttat tttccttatc ttgcaacgcc aagcgataca ttttaaaagc ttctatgtca
    15481 tcagaaacca gttgatacgt cggaagaaat tgttttatgt atgctttgcg atcattcata
    15541 ctcgtatctt tgacagctaa aaaagcacga ccggcttcca cgacagccga cgccacatcc
    15601 ttcggcattt ggtcaaattt gtatttggaa cgcgcttctt cgcgtataaa agtaaatcgc
    15661 gcaaacagtg tcatcggata tttattgtca atggattcgg cttgctgaag atattccttc
    15721 gccacttcaa acttgccaaa ttcagcatac atcatagcaa cttctgcata ggctcgcggg
    15781 tgctggggat atttatcaat ggcatgatta taatatgtca aagccatcat ctcacttttc
    15841 atttcgactc ctgcacgacc atactcacaa agcgaatcag ccggcgcatt atacatcggc
    15901 attccaggcg ctattttcaa ttgcgcttga agcgtaacac tcaaaaacaa agcaatgaat
    15961 aaacttctaa acatacctct ccttaataac atttaatgag aaccaaaaaa atattggata
    16021 caatttatta attgtagtag ccaataaaat gccaaatatc atggattata aattaagaag
    16081 atagatatgc agtatttttt aatatgtcgc agatttgcga atgaacggtt atcaatgcgt
    16141 cgcaatttaa cgactattga tatcagctat caaatattgg ccacttcttc ttctaaaagt
    16201 tggcgctcat gcatcgcggc ataggtgcca ttttgctcca ctagatcatc gtgcgtaccc
    16261 gtttcaatga tcgcgccttc tttcaaaacg atgattaaat cggcatgctt taccgtttga
    16321 atacgatggc tgacaagtaa agttgttcga tttttcatca gaatcttcaa acgcttcaat
    16381 atctcatctt ctgtgtgtgt atcaacgctc gaaagcgcat cgtctagtat gagtatctta
    16441 ggttcgcgaa ttacggcacg tgctatcgaa aggcgttgtt tttgccctcc ggatagagtg
    16501 atacctcgtt cgccgatgat tgtttcatat ttttccggaa aatcctcaat attgcttcgc
    16561 agttgtgctg tatcaacggc ccaaatgact ttatccgtcg gcggattatc aacgccaaaa
    16621 actatgtttt gataaattgt atcggaaaac agaaaattat cttgagtaac catgccgatg
    16681 tgttgacgca aaatagacac cggaattgta ttgatatcac gatcgtcaat gaatagcatt
    16741 ccgtccggaa tatcatacaa acggggaatc agattgacca atgtactctt tccggaacct
    16801 gtcgcaccga cgattcccaa ggtcattccg ggatggatgc aaattgaaat atttttcaaa
    16861 acaggtatat cgcgatatga aaaattaaga ttacgaaatt ctatcttacc tgcaatgtca
    16921 gagtgtccgt tactttgatt gtttttatca ctgattgcgg gctgttcatc aaagatggca
    16981 ttcattcgct tcatggaagc cgacccgcgt tgaaaaatat taatcaccca tccgacggct
    17041 accattggcc agatcagcat cgcgagataa aaattaaaag ccgtaaattc accaatggac
    17101 attacaccgt ctatcactaa acttccgccg taccacagca aaaccgcaac actcagccct
    17161 atggtcaaaa tcacactgga catcatgatc ccgcgaattc gaaccatgga catattgagc
    17221 tggatatact catgactgag attttcaaac ttcttaatct ccgcggcttc ttggacaaat
    17281 gattttacta cacggattcc ggagaagttt tcctgtgcag ccgaacttat atcggaaagt
    17341 ttttcctgaa ctttttgcga gcgcgtcatg atcacaccag atagacgccc taccgcaaac
    17401 gaaagtaaga ataaaggcag gaaagccagt aacgtaagca tcggactaat atgtatcatc
    17461 atcggtacga cgtatataaa cgctacaatc gtattggcgg tgtacataat gccgggccct
    17521 agcacttctc gtacattggc cagatcgttg gtcagacggc tcatcaggtc gcccgttttg
    17581 gcatactgaa aaaactgaac gtgtaatttt tgcaaatgcg aaaaaaaatc actgcgcagg
    17641 tcatactcga tctttcgtga catgacgatg agcgtttgtc gcatcagata aagaaaaatg
    17701 ccgtgtaaca attcgaccaa aaggtacgcc gcaatgtatg ccacaacgat ggccacacca
    17761 agttgtaact cgacggaatg gatgatttga ttgtatagag gaaatgttga aggatccgtg
    17821 ccgggcgcta atgtatcaat agcttcacgt accaactgag ggcctgtaga ctgaaataaa
    17881 ttttttaaaa taaccagcac aaatccgcta atccaataaa cgcggtattt ccaaaggtac
    17941 ggcttcaggc gcttaaacat agaatcttat tctttggtat aaaggttgtt ttttttgata
    18001 tacgataata ctgcattcgg tacgatatag cgaaccgatt gatttcgtaa aagtcgttca
    18061 cgcacccatg aagaagcaat ctcaatcaca ggtttatcaa tacatatcgc ttcttttgtg
    18121 aattgcaatt cgcctttatc gaagtcaaaa cccatacgcg gatatacgac gattttagaa
    18181 agttcagtca acctctccgg tgttttccaa ttaggtagat caagtaacga atccgatccg
    18241 atcatcagaa aatattcgtt tttaggattc tcttttcgta aaaactccaa cgtatgaacc
    18301 gtatacgaga gaccggatag tttttcttca atgtcagaac attggaaata ctcattttgt
    18361 tcaatagcta aatccaccat cgttttgcga tgatgaaacg agattacgtt ttcagaagtt
    18421 ttatgcggtg gccgtgctga ggggataaat accactttgg acaaaccgag atgatcgcga
    18481 ataatctcag cgatgatcag atgacctgcg tggatggggt taaatgttcc cccaaataaa
    18541 ccgatctttt ccaaatggtg tatttaattt gccgatttga cagaagtgga ctgttcaggc
    18601 ttcactaatt tagcctcttt ggccgcttta gcttgggcct tgagttcatc cttctttata
    18661 tcttccagtg tttcgagggc atccgttacc cactcttttt ctaacgggta ttcatcgatg
    18721 aattttttta ataaaatctg agctttttga aattcctcga ttttatagtt gcagtatcct
    18781 ttccaatact tggcttgcat agcatacggc gagtcataat aatcggtaat aacctgatca
    18841 taataaacaa tggcggcttc aaactcactg attttacggt aaatctgtgc gctttcaaaa
    18901 tgtttacgtg ctaatttgtc ttttaccgat ttataatgtt tatccatctc agtgcgcagt
    18961 tcgctgcgtg gaaacaaatc aataaaattc tgaatggcat tttgcgcctg ataggtgaac
    19021 ttctgatcca aggcaaatcc cggcgagagt tcgatatagc acaacgccaa ctgatacata
    19081 cttttttcaa taaattcgct tttgggataa cttgacacca tgcgttcaaa ttctgaagaa
    19141 gccgaaaccc aatctttttg acgataatag ctttcagcca aataaaactg cgcatcgtcg
    19201 gcgatgtccg aaccgctgta tttataggtt atcaaagaaa actgttcctg tgccgaagtc
    19261 caattttttt tattaaaaaa cttcattgcg tattcaaaat gttcccgtga tgtcatgtac
    19321 tgtgtaacat cttttttgct gcatccggta gactgtaaaa atgaacaagc catcgctaaa
    19381 acaaaaatct ttctcaacat gagatatcct ttacaaaatg gtatatatcg ttatgaataa
    19441 aatttcaaaa aatcaaatat catataacgc atagtcaccg ttgcgcgaaa caacgatcat
    19501 agtttcttct ttcattagcg tccatccgtc attgttgtct aaggcctccg aagtgaccag
    19561 tgtgccgcct tgatatgaag tataataacc caaactatgc attactttag ttgcgatcag
    19621 gtagtcccgc gtaagcatca taaaatttaa ttgcggtttt cgcgatgcgt ccgcacggcg
    19681 actgatttct ctaagatcgg ctattgtatg ctgcaatgat gtacgtacga aatggatgtc
    19741 cagtgtttgc gctggattat atttacgaag atacgtcaga aaaagatgca tggcatattc
    19801 tgaatccgta ttccccgtaa tatgtccgct aagcgcggga tcaattttgg ctaatgccga
    19861 atccataacg ttttcaattc cgtaaaccga cccgttgtga gcaaaaataa tgtcctgatg
    19921 atatatccag ggatgtgtat tgaccttgga tgcaccgcct accgatgccc ggcgtatatg
    19981 caacatgaca cgtttgaacg gccgatgggc tgtatccaaa aaaagtttat cgttacgggg
    20041 tgccgaagct tcctttataa cgtggaattg tccattttct tcgaaaccaa atccccatcc
    20101 gtcgtcgttg ggtttacaac tgtcacgatc gcatacgctt tgccggagaa ttccgttagg
    20161 tgcatcaaga attacgtttt gtaatgatac cggattcggc ccggcatacc ccatgagtct
    20221 gcacatcagt gttttatcaa ttcaaaacaa tgtttaagat agactttaaa tttttcagag
    20281 ttagccaaac gatcatattc aattttcaaa atcgttgccg gttttttatt ttgatcaagt
    20341 tgatactgca ttcgtttaaa ggaatctaaa tattttcctt gcaaagtttc aataaacggg
    20401 cggaacctat cagcgttttt ggaatcaccc gataactgtt gtacgacccc ctcaatgacc
    20461 gtgaccgttt taccgatatg actattgaga tcacttttga ttgtttccaa agcgctttta
    20521 taaataatcc aatctttgtg cgtggatgtc tcggcataac ccatcgcctg tgaaacataa
    20581 ttttgaaacc gctcatcatt catcaacgtc ataaactcgg ctttgacttg aggcgctgat
    20641 ttttttccgg cttcgatgga atctttatta tcattcaccg caaaataatc atcatcacgg
    20701 agcttgccta tcacttcttc atgctctgcg tttttatcat catctagcgc atcaagcagc
    20761 ttaccaagaa aacgatggta ttcgtcgatc aaaggcacga cttcttgttt agcgcgaatt
    20821 tgtgtatttt caaaatgacg ccaaccgatc gcatcctgca aaagcgctaa aatcagattg
    20881 accgcacgtt gggtatgacg aggatattca ggacgatcca aacgacgcgt catgaggtac
    20941 gtgccataat tttttatatc caaaagcgtc tcttcaaacg tcccttcttc ttccgcttta
    21001 gctaaaacgg actccggaat aatatttttc ttcactgcat cggattcgat tgcacgaatg
    21061 gattcctctt tgaatacggc ctttaccttg gcctcaacgt atttgacatt aaacgcatca
    21121 ccgtcatccg aacccttagt gatcgtgccg acacgaacat aatctgcaac agctaatccc
    21181 agttccgcat cgttgatttg atctagtatt ctatggacac cgggtttatc aagcgtgaga
    21241 cgttctttat caataaaatc ggcaacttgg ttgattgcat taaacgcgcg ttcggcactg
    21301 ttaaaaaaac caagccgttt acgtatttca tgaaaatgac tgatgcgttg attgagttcg
    21361 tcttcatcca cggtcatatc cggcggcatt ttagtcagat ctaaaccaat gtaatcggta
    21421 gataatttca gatagctagg atcaatatca aatttggaca taagccgcgc tgaaaggtat
    21481 cgtgcggcca aggctgcttt tttgatatca gaatcttctt ctttcatcac gcctttatca
    21541 atatgcggca attcgtattt aaaatattcg acgacgcgac gataatcatc ttttttggcg
    21601 tccagttctc cgatgagttc gacgaattcg tacacttctt tgatttgaaa acctttaatt
    21661 ttaaaaatca ttgatgtgag gtactgcgtt tccgcggcaa tctcgaccaa tttggccgga
    21721 tcgtgcaatg attcgtcagg attatcaaga cgacgcgcaa aagaaatagc cgctatcgtc
    21781 tgatccgcga tattatccgc cgccagtaca cgacgaattt cctctttgat ctcatcgtcg
    21841 agggtaggaa aattatcttg tgccagcttt tcttcgtatt gtgccaaggc aacattgatt
    21901 tcaagtgaat tgataagctg taaccattct aatttctcgg tttttgaacc aagttcaata
    21961 ttcagatcgc cggacaatgt cgtttcgata atatggtcga gtattcgaaa ttgacgtttg
    22021 atattattcg attgccccaa acgattgatg ataatgcctt ccagcgtatc gttaggataa
    22081 ttgtattccg ct
//
LOCUS       DAOQGF010000049        21903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1095_length_21903_cov_7.233797, whole genome shotgun sequence.
ACCESSION   DAOQGF010000049 DAOQGF010000000
VERSION     DAOQGF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21903
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_21903_cov_7.233797"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1647
                     /locus_tag="PK937_08415"
     CDS             343..1647
                     /locus_tag="PK937_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32632.1"
                     /translation="MKRISVFAVIFTFLFSALPLFSQIGSSSAITDRYGSMFTYSAKT
                     LKFPQYYFYQNNKLFIGTANRGIGASTKVKDYGAQLGLNFGFTESFDVIINGNFVQTP
                     NRASSIPKNKIVGFFNSVDVPDEFYLNLRYIPWVWKDGQYRLGFMLTRKFNGNGFPNA
                     PFQVYQTESPDVGLSLIGSYFAMPGIEDNGLMAHINLQYWNHLDAGKYIAHNDEDDVR
                     EAVNSGGKSLADTSVVESNTSSLRFSLGAQYPLFLGGRYLFLTGDVYGLFYLQKPPVA
                     AYSRQNYAYLALGAKYQAFNWMAVHVGADFRVLSGSDKTISSDLLEINDLTIGKSDYP
                     TWHFYAGVTVPLEPRVRVVRANDESELYARQSKIKKNEVKDILYSEQEIQKRSQNFIP
                     LKEMRRSYKSIIGNLLGVLEPKDKKPVEPEPPAEEQSAEGQN"
     gene            1788..2981
                     /locus_tag="PK937_08420"
     CDS             1788..2981
                     /locus_tag="PK937_08420"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNH32633.1"
                     /translation="MKLNKEIVIVAGARTAFGKFGGTLKDFSAIQLGVFAAQETIKRA
                     GVKPEDVDHVVFGNASQTSADAIYMARHIALKAGLPQTTPALTLNRLCGSGFEAIIEG
                     ARQILLGESTMVLAGGTESMSQAPFVVRGTRFGVNLGQPMKFEDSLWEALSDPHCGFS
                     MAQTAENLADKLKISRTDCDLYAYLSQMRAKDAILGGRLKDEIAPIEIKTRKGVKVFD
                     TDEHPRPETTVEMLQTLAPYFKKDGTISAGNASGICDGAAAVLITTAEIAKAKGLKPL
                     GRLISWGHAGVDPSIMGIGPVPSSQKALADAGLKVSDMDIVEINEAFAPQYIACEKEL
                     GLNREITNVNGGAIALGHPLAASGARLTLTALYELKRRGKKYGLASACIGGGQGVAVV
                     IEALS"
     gene            3066..4571
                     /locus_tag="PK937_08425"
     CDS             3066..4571
                     /locus_tag="PK937_08425"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32634.1"
                     /translation="MKNSIFVAGMIFFLISCGQEKKHMSIKEFPVAVDSIFQKSCAIA
                     GCHLPDEHDLAKALHLPNNLDLSSWSGLFEGGENGAAVVAYRADKSHMMGHIRGVMDP
                     RMPPNYAPYNKDTLVASDVTIIQSWIEDGAPSLEGNIPFEHVRNKIFTTNQINDIVSV
                     LDARTHNIMRVFDVGDRPQNESPHGIEVSRDGKYFYTSMIATGDVFKYDAKTGELIDK
                     AALLDPVALIKLSQDGSKLYVTTKFEVNNTGNNGRITVLNTETMSEIKSIPVGVSPHG
                     INLSKDGTLLYVSAVYSDRIYVINTQADTLATHFDVADDVTPTPKYEPYHIGMGPKNN
                     SGYENLLFITCRKNAQVRIFQRTKTNNGNTFTFKQAINVGHNTNAKPIQLDVLPDGSA
                     VFVANSNDSSITVIKKNAEGYYKETDITAQTSDDGKTFHRLAQPNGVSVSPDGSLVYV
                     SNRNKDGAIIPHHGGSGGTGLITVIDVSTNKILKTLEVDPDAYSVFVSYPR"
     gene            4648..5934
                     /gene="glyA"
                     /locus_tag="PK937_08430"
     CDS             4648..5934
                     /gene="glyA"
                     /locus_tag="PK937_08430"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNH32635.1"
                     /translation="MEHIHQFDPELYKAIADEKVRQNTTLEMIASENFVSKAVMEAAG
                     TVMTNKYAEGYPGNRYYGGCEFVDVAENLARDRAKELFGAAYANVQPHSGSQANMAVY
                     FTLIKPGDTVMGMDLAHGGHLTHGSPVNFSGKMYKVVSYGVKKETGRIDYDQMVRVAR
                     EHKPKLIIAGASAYTRFFEYEKFRAIADEIGAYLMADMAHPAGLIAAGLHPSPVPYCD
                     IVTTTTHKTLRGPRSGLILMGKDFENRLGITAPKSGRLRMMSEILDSNVMPGIQGGPL
                     MHIIAAKAIAFKEALQPSFKEYARQVMANAKALAEQLVNLNYTLVSGGTDNHLVLVDL
                     SNKNITGKKAEQTLEHAGITVNKNMVPFDVQSPLVTSGIRIGTPALTTRGMKESEMRI
                     IAGFIDRAIRHPDQMAELHKIKNEITDLTKNFPLYA"
     gene            5969..6451
                     /locus_tag="PK937_08435"
     CDS             5969..6451
                     /locus_tag="PK937_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32636.1"
                     /translation="MLSVLLALFYLSCSGSKNIRKTTETKSDAEAPPVMVESPKVMRW
                     YIVVEQKAGLYKRYVELSYDGQMSQLTEGGRGPASVVFFAADTLRRLDRDIAEMIGEK
                     VSTSDRQDAILCLTVYSESGDAVLDTCWTQTSWENSSASARRLVEWISRQTDNFISIK
                     "
     gene            complement(6575..7279)
                     /locus_tag="PK937_08440"
     CDS             complement(6575..7279)
                     /locus_tag="PK937_08440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNH32637.1"
                     /translation="MKIKDISLTISNDIVTWPGDPKVNIQLPIQMKKGDACNVSRWEI
                     GAHTGTHTDAPFHFVDDGKGIDDVPLDIYIGPCIVVEVKPKTINIEADDLKHIDFKGH
                     KRVLFKTKNSLHWKNGNMEFDKDFIAVGLTGAEYLLKKKVKLVGVDYLSVESFHAEFD
                     HPVHKKLLGSHVVVVEGLNLSDVRPGEYELICMPLKIKHGDGTPVRAALRDLPNAKVK
                     SKPKKQTAANKKRKKK"
     gene            complement(7353..8558)
                     /locus_tag="PK937_08445"
     CDS             complement(7353..8558)
                     /locus_tag="PK937_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH32638.1"
                     /translation="MNLDFQNKYDELINSLPDGIFVINENGQIILANQSAAEILGYYG
                     PSEFYGLTVDTIYANTGDHDALLSILGHRGYAERSLFDWRKKDGSTRLIEVSAIPLRN
                     AQGKIGGIQAVFRDISRRLENQIAQQETIAEAASRAVDADRILEYINFYRTRPMSLIL
                     QGVAHNLNTPLGSMRGRAELLLHQLRKNASLFESVSDEKLRSDLDGFRVKLEKGLNEV
                     IGQVDKASLLMRGFSAKVAFEMQDAEMELDINAILQNEFGFFESSLYFKHQIQKEMQY
                     ADKLPMIKGIYRDFSQAFHNIIIHCIKATAAISDRWLYVQTELIDNMITVTIRDNRVM
                     AAHDSEAYHGRMNLETRYSALSDENFFSMGDIELFNARQYLAKYQATMTLFPNGEPKI
                     IVQIPRNWN"
     gene            complement(8565..9473)
                     /gene="prmC"
                     /locus_tag="PK937_08450"
     CDS             complement(8565..9473)
                     /gene="prmC"
                     /locus_tag="PK937_08450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNH32639.1"
                     /translation="MSSAVPEIWTVKSIMDWTIHYLSEKNIESARTNVEWLLCHTLGC
                     KRMDLYVNYDRPLDQNERDEFKASLKRRLLAEPLQYIVGHTDFMGLTFHVNRNVLIPR
                     PDTELLVERVLDLCGKSENIKSLRILDIGTGSGAIIISLAYYLKKRGFELQATAVDIS
                     QNALVVAQENANKLLPGFHIQWQHGDIFDPSSIESLKHRFDIIVSNPPYISDKEYALL
                     DKEIRDYEPVTALRAEDNGLAFYRRIGHIAADILDPSSAFGYIALEVGFDQAEAVAQI
                     LHSAGYTYQQCYKDIQNYERVLIGKK"
     gene            complement(9477..10010)
                     /locus_tag="PK937_08455"
     CDS             complement(9477..10010)
                     /locus_tag="PK937_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32640.1"
                     /translation="MQKILCWVGIIPFTISAAIFIPALMSDLITGNYTTTEQSKLSAL
                     QNDSLVALSDNPHSQKPTELGALIGAGICMGIVPGILTFLLIVQLRKINRLEKKHNEV
                     YIQDRLLELAQKLNGKLTIVETATELDIAVSDAQTLLENLCRIGMAQLQVSESGTLVY
                     HVTDIISKDEKDRSETV"
     gene            complement(10043..10831)
                     /gene="thiD"
                     /locus_tag="PK937_08460"
     CDS             complement(10043..10831)
                     /gene="thiD"
                     /locus_tag="PK937_08460"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNH32641.1"
                     /translation="MNPRKALTIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQ
                     NTVGVTAIMDVSTDVISRQIEAVITDIKPQAVKTGMLSTADIIQTVSDSLSKYGVKKL
                     VVDPVMVAKSGDRLLQDNAISALIERLLPQATVLTPNIPEAEVLTGKKITTISDMEIA
                     ADTLRAMGPKYIIVKGGHLKGEAIDVFYDGRKIYHLRHQRIRSKNTHGTGCTFSAAIA
                     AHLAKGFTVLESVRLAKRYISMAITHAFSVGSGHGPVHHFHKFY"
     gene            complement(10847..12622)
                     /locus_tag="PK937_08465"
     CDS             complement(10847..12622)
                     /locus_tag="PK937_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNH32642.1"
                     /translation="MKYSDHPIVQKLLEEIVSHLTDLSADRTDRIIGLVRSFTENQLV
                     HEKKLTRIGSALSSERNLDALLEMIVVEAMSFTNADGGTLYIKTPDDRGIAFKIMRTK
                     SLNFAMGGTSGKTIPFPPVKLIKDDGTRNEQQVSAYVALTGNTVNIPDVYDVEGFDFQ
                     GTKAFDKNTGYRSKSMMVIAMRNHENEIIGVVQLLNAKAHDGTTIPFSVDYQELIESL
                     ASQAAVAITNTTLINDLRALLDSFIQVIAAAIDEKSPYTGGHIRRVAELTSAMARSMS
                     DTSEGKYKDFTMNEDEHNELRIAGWMHDIGKITTPEYVVDKSTKLETIYDRIHTVMAK
                     FEVLKREEEVRYIRERAKIETSKARDKKKKLSSLEESYAQKKKQIEDDAAFIKVCNIG
                     GEFMEDAKLERLRNIAQQKIIMEGQEMQLLTENEVYNLSIRRGTLTEEERKKIQDHVV
                     LTIKMLQQLPWPNKLSRVTEWAGAHHEALNGKGYPYGLTAEQLNTQQRIMAVADVFEA
                     LTAADRPYKPGKKISECIKILGFMVKDGHLDPDIVDYFITTGMAQDYARRELKDYQLD
                     VFTYNGKEYDCRRPAQEPTPETATS"
     gene            complement(12698..13255)
                     /locus_tag="PK937_08470"
     CDS             complement(12698..13255)
                     /locus_tag="PK937_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNH32643.1"
                     /translation="MNSKSKAIAVDAGMLKDFLLVIGFSILTAVSAWVEVPLFFTPVP
                     ITLQTLMVSLSGALLGARRGALSQAAYLFYGICGLPVFAGGASTIAYLIGPTGGYLLA
                     FPIMAFLTGMLVMKESNLLYNIGAFFLASLPVLLIGTLQLKLYTGQGMATAFSLGFLP
                     FIAGDFIKCTLAAVIHKSWNAYRSR"
     gene            complement(13371..14999)
                     /gene="rny"
                     /locus_tag="PK937_08475"
     CDS             complement(13371..14999)
                     /gene="rny"
                     /locus_tag="PK937_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNH32644.1"
                     /translation="MDSLSVIIMCAAGIVMFILGWVFATYFGKNKVSEIRKIAENTIR
                     EAKREAETIRNEAEVKSKQRWHQIKMKIVSETDEREKRLKQYEQKLVNREREIRNKMS
                     GTVEKEQLLQRSQKDLTTQQEQLQTRAQHVESVIHQQMEKLENITKLSYEEAKKQFLE
                     SVAQRAQKEAAELMLQMKNEATQKASFEAKWIISDAIQRLASDQTAEITLTTVDLPNE
                     KIKGAIIGREGRNIKAFEQITGVKVIIDDTPEVLVLSSMDPVKREIARLAMLRLTETP
                     NIQPEFIEDVVERCKKQVETAMNKASDEVLNSLNIKNVAPEMKQMLGRLKYRTSYGQN
                     VLQHSKEVALLAGNMASELGYDVQLARRAGLFHDIGKATSNENEGSHVTIGVEVTTRC
                     KEHEVVINSVLAHHDEAEPIHPISQLVTAADIISGSRPGARREPLEAYGARIEKLEQI
                     AQQFDGVSKVYAIFAGREIRIVVEAEKMDDAQSAMLSSNVAQKIQNEMQYPGQIKVVV
                     IRERRVVRYTNSTSPAVDEARIEEAVVSDSPSQN"
     gene            complement(15201..>16098)
                     /locus_tag="PK937_08480"
     CDS             complement(15201..>16098)
                     /locus_tag="PK937_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH32645.1"
                     /translation="LHVLPPFWKTWYFRLAIVVLITLALWSFHRLRLQRVEQYNRRLE
                     KIINERTTELKLKNEELEVLNQKKNEFLGIAAHDLRNPLTTIIGYVQLMINDIRHGHA
                     TMKDVENDLDTVHKVSQQMASLVSTLLDWSSIEAGKVNLEKHSESLQRIIDECENLHR
                     RAAEQKNIRLAVERPLLMPNVFVDRNRILEVMDNLLSNAIKYTHPGGAVRVFCEPTDR
                     EICVHVEDTGQGLSQEDLNKVFKSYQKLSARPTAGEVSTGLGLAIVKKVIELHGGRVW
                     VTSEKGKGSRFSFSVPRYTEYN"
     assembly_gap    16099..16198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16199..17979)
                     /locus_tag="PK937_08485"
     CDS             complement(<16199..17979)
                     /locus_tag="PK937_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32646.1"
                     /translation="MIRFGVIIKSLLIFATGYWLNPAPQNTYAQSLPDHFSQLTVENG
                     LSHSTIYTVYQDRLGFIWMGTENGLNAYDGYRFIHFKHDPRDSTTLPNNNVTSIVEDD
                     EARLWLGTWGGGLARFDRTTSTFKTYKPIAGLNHTLSDLRIQYILRRSDGKLWIGTNS
                     GGVNIFDPVTEKFVHVSHDPHSEASLPHPRVWSLAEQNHAVWVGTGNGLCRVSETPDG
                     LKTERIEKIYPGLVLSHGTIRALFADGDSLLWVGTQDGLDRVDLRTASVRRSLTHERV
                     NVVTRLQNGHLAAGTIQHGMFIMDSDGTVIKTYHHDPNYAHSLGHNDVRWIMEDRSGI
                     VWIATRGEGVSLLDSKPPKFSIVADDIQNNTLRSTRVAAVFEDHSANGKLVLAGTDRG
                     LIVYNEKKRSFTTYLEPTEDNTSSVWITSIYKDPAGFYWIGTFGEGLIWFDLQRGVLK
                     KIVSRPGDSTSLNDNRVRTIYAQSINGVRRLWIGTYGGLHLWDESNNRFKRWVAQNGN
                     PNSLSHNFIRTIVSDHVNRLWIGTYGGGLNRFEPETNNWKHFKGNPTYKNSISGNEIM
                     TIALDPDTAHPHLWIGTDGNGISLMDIR"
     gene            complement(18110..19453)
                     /gene="accC"
                     /locus_tag="PK937_08490"
     CDS             complement(18110..19453)
                     /gene="accC"
                     /locus_tag="PK937_08490"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNH32647.1"
                     /translation="MFKKILIANRGEIALRVIRACREMGIKTVAVYSEADANSLHVRF
                     ADEAICVGPAPSAKSYLNIPQIMSAAEITGADGIHPGYGFLSENIKFAEICESVGVKF
                     IGPKSHMIQKMGDKAFAKDTMKKAGVPVVPGSDGIVRDIKHAREIMKEAGLPVIIKAT
                     AGGGGRGMRIVREESEFENAYNTARAEAESAFGNPDVYIEKFIEKPRHIEIQLIGDQH
                     GNVFHIGERDCSMQRRQQKLIEESPSPAVDEALRQKIGEAAVRGAKSVNYESVGTIEF
                     LMDKEKNFYFMEMNTRIQVEHCVTEMVYGIDLIKWQIRVAAGEKLPFQQSDFKPRGHA
                     IECRINAEDPDHNFRPSAGKIHAFHIPSGYGIRVDSHAYAEYQIPSNYDSMIGKLIVL
                     GENRTEAISKMCGALDEFIIEGIKTTIPFQKRMMLNENFVSGDFDTKFVERLLGL"
     gene            complement(19517..20464)
                     /locus_tag="PK937_08495"
     CDS             complement(19517..20464)
                     /locus_tag="PK937_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32648.1"
                     /translation="MDFREKILMIDDEPDKIFIFSTILQNKGFEVITSESGREGLEIM
                     TRIRPDLILLDINMPDINGHTVREKLKERPSTRNVPIVMFSSSDQLPDKLASLRNGAD
                     DYITKDVDHQELTARLEALIRRYKESIGANPLTKLPGNYAIEDDINFRIAQNQKQKTP
                     FAVCYADLDNFKAYNDKYGFKSGDEAIQGTARIILDVIGELGAKEDFVGHVGGDDFVF
                     IVSRYDRVETICKTIIERLDDFYPSLYDETDRKAGHIITKNRKDEMDQFPLMSISIAV
                     VSNENRELSSMGQIAQIAAEVKKAVKKKDGSNYLIDKRS"
     gene            complement(20571..21230)
                     /locus_tag="PK937_08500"
     CDS             complement(20571..21230)
                     /locus_tag="PK937_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNH32649.1"
                     /translation="METGTILGFLKKLKRNNKKTWFDAHRDDYAMAKMSFENFVGDLI
                     GDMTVVDPSLASLQPKDCIFRIFRDTRFSKDKTPYKTNFGAFLASGGRKSSGAGYYIH
                     IEPGASMLGGGLYMPPSDILLKMRNYVAEEADGLKKIIKAPAFKKYFNELQPEKLKTM
                     PKGFPASHPHAEWLKYTSYVVLHKISDKELSNPRFEEHVIKGFKAMKPLCDFINDAVS
                     Q"
     gene            21422..>21903
                     /locus_tag="PK937_08505"
     CDS             21422..>21903
                     /locus_tag="PK937_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNH32650.1"
                     /translation="MSEVFSGILETDPKGFGFIRTLSLTLSPSPKDVFVPPGIITQNR
                     LRPGVLVEGTGAPGDKGNTRVATITRINGMEPEAWKSVQDFGTLQVIQPRDRISLTGH
                     EQNTSMRVVDLFCPIGRGQRGLIVAPPKAGKTRLLQELAHAISMNNDDITMIVLLIDE
                     R"
BASE COUNT         5802 a         4815 c         4955 g         6231 t
ORIGIN      
        1 tcgagaaact ccttacgttc aatacgaagc ggcggaaccc aattcggatc atcggcgtag
       61 attttccatt gaaacgaaat aaatttatcc agctgcttga aattaagttc tttgatggtt
      121 acagacatgt tgcttttctt tttgaaaatg atgcaaacat acataccgtg tgttttactt
      181 tcaataagat atttcaaccg gaaagtaggt tttgaggaaa tcgcttctat aatgaagttg
      241 agcaaaatca taaaaccagt ttttaaaaat cgcttgcttt tataaacccc ttattttact
      301 ttactttgct ttcaaatttc catgttgttt cggagcgctt gtatgaaacg gatttctgtt
      361 tttgcagtca tttttacttt ccttttttct gcgttaccgc tgtttagtca gatcggtagt
      421 tcgtcggcaa tcacggatcg ttacgggtcc atgtttactt actctgcgaa gacattaaag
      481 tttcctcaat attatttcta tcagaataat aaattgttta tcggaactgc gaatcgcggt
      541 atcggtgcat caaccaaagt taaagattac ggcgcacaac tgggtttgaa cttcggattc
      601 acagagtcct tcgacgttat catcaatggt aattttgtac aaacacctaa ccgtgcatca
      661 agtataccaa aaaataaaat cgttggtttt ttcaattcag tagatgtgcc ggatgagttt
      721 tatttaaatc tgcgctatat accgtgggta tggaaagacg gtcaatatcg tctcggtttt
      781 atgttaacgc gtaagtttaa tggcaacggt tttcccaatg cgccatttca ggtatatcaa
      841 acagaatcgc cggatgtcgg gttaagttta atcggttctt attttgccat gccgggcatt
      901 gaagataacg gattgatggc gcatatcaac cttcagtact ggaatcactt ggatgcaggt
      961 aaatacattg cccataacga tgaagatgat gtgcgcgaag ctgtaaacag cggtggtaaa
     1021 tcattggcag atacatcggt tgtcgaatca aacacctcta gccttcgttt ttcgcttggt
     1081 gcgcaatatc ctctgttttt aggcggtcgt tatctttttc tgacagggga tgtttatgga
     1141 ttgttttatt tacaaaaacc cccggtagca gcctatagcc gacaaaatta tgcgtacctt
     1201 gctttagggg caaaatatca agcgtttaat tggatggccg tgcacgttgg tgcagatttc
     1261 cgagtattat caggttcaga taaaactatt tccagcgatc tgcttgagat caacgatctt
     1321 actatcggta agtcagatta tcctacttgg catttttacg ccggcgtgac ggtgccctta
     1381 gaaccgcggg ttcgcgtggt tcgggccaat gatgagagtg aactttacgc ccgccaatcg
     1441 aagatcaaaa aaaatgaagt gaaagatatt ctgtattccg agcaggaaat ccagaaacgc
     1501 agtcagaatt ttattccact caaagaaatg cgccgcagtt ataagtctat tatcggtaac
     1561 ctgcttggcg tgctggaacc caaagataaa aaaccggttg aaccggaacc tccggccgaa
     1621 gaacaaagcg ccgaaggaca aaattaattt tttagtctta ttccaaaaac ccgcacaaca
     1681 aaaatgtgcg ggttttttgt tttggggtgg aatttattta tcaaaaacac tattctttcc
     1741 tcgtatcaaa aaaaccaaat atcattcttt caatcggagt tttttagatg aaattgaaca
     1801 aagaaattgt aatagtcgcc ggtgcacgca cggcgtttgg aaaatttggc ggaacactga
     1861 aagatttttc cgctatccaa ctcggcgttt ttgctgcaca ggagaccatc aaacgcgcgg
     1921 gtgtcaaacc tgaagatgtg gatcacgtag tttttggcaa tgcatcacaa accagtgcag
     1981 atgcgatcta tatggcgcgt catatagcgc tcaaggcggg actgccacaa accacaccgg
     2041 ctttgacttt gaatcgcctt tgcggctccg gctttgaagc aatcatcgaa ggtgcgcgcc
     2101 aaattctttt gggcgaatcc acgatggttt tggcaggcgg tacggaaagt atgagccaag
     2161 ctccgtttgt tgttcggggc acgcgatttg gagtcaatct tggtcaaccc atgaaatttg
     2221 aagattcatt gtgggaagcg ttatccgatc cgcactgcgg tttttcgatg gcgcagacag
     2281 ccgaaaactt agccgataaa ttaaaaattt cgcgcacgga ttgtgatctt tatgcgtacc
     2341 ttagccaaat gcgagcaaag gacgcaatac ttggcggacg actcaaagat gaaattgctc
     2401 ctatcgaaat aaaaacccgc aaaggtgtaa aggtttttga cacggatgaa catccccggc
     2461 cggaaacgac agtcgaaatg cttcaaacac ttgcaccgta tttcaaaaaa gacggcacga
     2521 tatcagcggg caatgccagc ggaatttgtg acggtgctgc agccgtactt attactaccg
     2581 ctgagatagc caaagcaaaa gggctaaagc cgttgggtcg cctgataagc tggggtcacg
     2641 ccggcgttga tccttcgatc atgggtatcg gtcccgtgcc ttcttcacaa aaagcgcttg
     2701 cggatgccgg gctcaaagtt tctgatatgg acatcgtgga aatcaatgaa gcttttgcgc
     2761 cgcaatatat cgcttgcgaa aaagaacttg gcctgaaccg cgaaattacc aacgtaaacg
     2821 ggggcgctat tgcgctcggg catccgctgg ctgcatcggg tgcgcggctt acattaactg
     2881 cgttatacga attgaagcgt cgtggcaaaa aatacggttt ggcttctgca tgtatcggtg
     2941 gcgggcaagg cgtcgccgta gtgatagaag cgctatcgta atcaaactaa aatcatatca
     3001 aatatgacgt gttttatact attcccgcca caacagcggg aatagtgctt taggaaggac
     3061 aacgaatgaa aaactctatc tttgtggcgg gtatgatttt ctttcttatt tcatgcgggc
     3121 aagaaaaaaa acacatgtcc ataaaagaat ttccggtggc agtggattcg atttttcaaa
     3181 aatcgtgcgc tatcgcagga tgccatttgc ccgacgaaca tgatcttgcc aaagctcttc
     3241 acctgcctaa taatttggat ttatcatcat ggagcggttt gtttgaaggt ggtgaaaacg
     3301 gcgcggcagt cgtcgcgtat cgtgcggaca aaagccatat gatgggacat attcgtggtg
     3361 ttatggatcc gcgtatgccg ccaaactacg caccgtacaa caaagataca ctggtcgcat
     3421 cggatgttac cataatacaa tcctggatcg aagatggtgc accgagtctt gaagggaata
     3481 ttccttttga acatgttcga aataaaatat ttacgaccaa ccagattaat gacattgttt
     3541 ctgtgctgga tgcacgaacg cacaatataa tgcgcgtgtt tgatgtcggg gatcgcccac
     3601 aaaacgaaag cccgcacggg atcgaagtga gccgtgacgg gaaatatttc tacacatcca
     3661 tgattgcaac cggcgatgtt tttaaatacg atgctaaaac cggcgaatta atagacaagg
     3721 cggcgttact ggatcctgtc gcgttaataa aactttcaca agacggatcg aagctgtatg
     3781 tgaccaccaa atttgaagta aataatacag ggaacaacgg acgtatcacc gtgctgaata
     3841 cggaaacgat gtcggagata aaatctatcc ctgtgggcgt tagcccgcac ggaattaatc
     3901 tttctaaaga cggcactttg ctgtatgtca gtgcagtgta ttcggatcga atctatgtca
     3961 tcaataccca agccgacacg cttgctactc attttgatgt ggcggacgat gtaacgccaa
     4021 cgcccaaata cgaaccttat cacatcggga tgggtccgaa aaataacagt ggttatgaaa
     4081 atttgctttt tattacgtgt cgaaaaaatg cgcaggtacg tattttccaa cgtactaaaa
     4141 caaataacgg taatacgttt acttttaaac aagccattaa tgtcggccac aataccaacg
     4201 caaaaccaat ccaattagac gtgttacctg atgggagcgc agtttttgtg gctaactcca
     4261 acgatagcag tattaccgtt ataaaaaaga atgcggaagg atattataag gaaacggata
     4321 ttaccgcgca gacttcggac gatggtaaaa catttcatcg ccttgctcaa cctaacggtg
     4381 tttccgtaag tcctgacggg tcgctcgtgt atgtgagtaa ccgaaataaa gacggcgcca
     4441 tcataccaca tcacggcggc agcggaggaa caggattgat taccgttatt gatgtttcta
     4501 caaataagat tttaaaaaca ttagaagttg atcccgacgc gtatagtgtt tttgtttctt
     4561 atccgagata agcggccaaa attttcattt gcaccttgat aggcttgatc actacattgt
     4621 cacgaacaaa aaaaggattt ctacttcatg gaacacattc accagtttga tcccgaattg
     4681 tacaaagcca tcgcggatga aaaagtacgt caaaatacga cgctcgaaat gatagcatct
     4741 gagaattttg tcagcaaagc cgtaatggaa gcggccggaa cagtaatgac caataagtat
     4801 gcggaaggct atcccggaaa tcgttattac ggtggatgtg aatttgtaga tgtcgccgaa
     4861 aatctggctc gggatcgtgc caaagaactt ttcggtgccg cgtatgccaa tgtgcagccg
     4921 cactccggaa gccaggctaa catggctgtg tattttactt tgatcaagcc aggcgatacg
     4981 gtgatgggta tggatctcgc gcatggtggt catttgacac acggcagtcc ggtaaatttt
     5041 tcaggaaaaa tgtacaaggt cgtatcgtat ggtgtaaaaa aagaaacggg ccgcattgat
     5101 tacgatcaaa tggtacgtgt ggcgcgcgag cataaaccga aattgatcat tgccggtgca
     5161 agcgcctata cacgtttttt tgaatacgaa aaatttcgcg caattgcgga tgaaattgga
     5221 gcttatctta tggccgacat ggcgcatccg gcaggcctca ttgcagccgg cttgcatccc
     5281 agtccggtac cgtattgcga tatcgtaacg acgacgacgc acaaaacgtt gcgcggcccg
     5341 cgttcggggc ttatcctgat gggaaaagat tttgaaaaca gattaggaat tacggctccc
     5401 aaatccggtc ggttacgcat gatgtctgaa atcctcgata gcaatgttat gcccggtatt
     5461 caaggtggcc cgctaatgca catcattgcc gcgaaagcta ttgcgtttaa agaagcgctt
     5521 cagccttcgt tcaaagaata tgcccgtcag gtgatggcta atgcgaaggc attagcagag
     5581 caactggtca atctgaatta tacactcgtt tccggcggta cagacaatca tttggtattg
     5641 gttgatctat ccaataaaaa tattaccgga aaaaaagccg agcaaacact tgagcatgcc
     5701 ggcataacgg taaataaaaa catggtgcct tttgatgtac aaagcccgct tgtaacaagc
     5761 ggtattcgta tcggcacgcc ggcgttgacg acacgcggta tgaaagaatc ggaaatgcga
     5821 attatcgccg gatttattga ccgggcgatt cgccaccccg accaaatggc agaactccat
     5881 aaaataaaaa atgaaatcac ggatcttact aaaaatttcc cgctctatgc atagattttt
     5941 attaaaaaca aaatcacaag gtgtgttttt gttgagtgta ctcttggcgt tgttttattt
     6001 gagttgttcc ggtagcaaaa acatcaggaa aacaactgaa actaagtctg atgctgaggc
     6061 gccacctgta atggtcgaat cacctaaggt tatgcgttgg tatatcgttg tagagcaaaa
     6121 agcaggtctc tataaacgct atgtagaact gtcctatgat ggtcaaatgt cccagcttac
     6181 ggaaggagga cgaggcccgg catcggttgt tttttttgcc gcagatactt tgcgtagatt
     6241 ggaccgcgat attgcagaga tgatcggtga aaaagtaagt acgtccgacc gacaggatgc
     6301 gattttatgt ctaacggttt atagcgaaag cggcgatgcc gtcttggata catgttggac
     6361 acaaacatcg tgggaaaact cttccgcatc cgcacgtcgt ttggtcgaat ggataagccg
     6421 gcaaacggat aattttattt ccatcaaata atttgaggag ggccggcgaa atctataagt
     6481 aaaaaattcg tcaacgcgaa taggtttact ctgggggccg gtccatattt gtaaaaaaac
     6541 ctcggtttta aagccgaggt tttttatgta aaatttattt ctttttgcgt tttttattgg
     6601 ccgcggtttg ctttttaggt tttgacttta ctttggcatt aggaagatca cggagcgctg
     6661 cgcgaaccgg cgttccgtcg ccgtgtttta ttttaagcgg catacaaatt agttcgtact
     6721 caccgggccg gacatccgaa agatttaaac cttcgacgac gaccacgtga ctgccaagta
     6781 attttttatg cacgggatga tcgaattcgg cgtgaaagct ttccacgctc aaataatcaa
     6841 caccgaccaa tttgactttt ttctttaaca aatattcagc gccggttaaa cctacggcaa
     6901 taaaatcttt atcaaactcc atattaccgt ttttccagtg caaggagttt ttggttttga
     6961 aaagaacacg tttatggcct ttgaaatcaa tgtgtttcaa atcgtcagct tcgatattaa
     7021 tcgttttagg ttttacttct acgacaatgc agggcccgat ataaatatcc aaaggcacat
     7081 catcaatacc tttgccgtcg tcaacaaaat gaaagggtgc atccgtgtgc gtgccggtat
     7141 gagcgcctat ttcccagcgt gatacattgc acgcgtcgcc ttttttcatt tgtatcggaa
     7201 gttgaatatt aactttagga tcgcccggcc aggttacgat gtcattggaa atcgtgagag
     7261 aaatgtcttt tattttcatg cgtcacctct ccaaaaaaat tatgattaat aaacacgaat
     7321 tacgaaaggc gtcgaaaaag aagggcgcgt gattaattcc aatttcgtgg aatttgcacg
     7381 ataatttttg gctctccgtt aggaaagagt gtcatcgtag cctggtattt ggccaaatat
     7441 tggcgagcat taaaaagttc gatatccccc atcgaaaaga aattttcatc tgaaagggcc
     7501 gagtagcgcg tctcaagatt catacgtcca tgatacgctt ccgaatcgtg cgcggccatt
     7561 acgcggttat cacgaatcgt aacggtaatc atattgtcaa tcaattccgt ttgcacataa
     7621 agccaacgat cagaaatggc ggctgttgct ttgatgcaat ggatgatgat attgtgaaat
     7681 gcttgtgaaa aatcgcgata aatacctttg atcatcggta atttatcagc gtactgcatt
     7741 tctttttgga tttgatgttt gaaatataga ctcgactcaa aaaatccgaa ctcattttgc
     7801 aaaatagcgt taatatccaa ttccatttcg gcatcttgca tttcaaaagc aactttagca
     7861 gaaaaacctc gcatcaaaag gctggcttta tctacctgac cgataacttc gttgagtcct
     7921 ttttccaatt ttacgcgaaa tccatccaaa tcgctgcgca gtttttcatc gctgaccgat
     7981 tcgaaaagcg atgcattttt tcgcagttga tggagaagta attcggcgcg tccgcgcata
     8041 ctaccgagcg gggtattcag attatgagcc accccttgga gtataagcga catcgggcgg
     8101 gtccggtaaa aattgatata ctccaaaatc cggtccgcat ctacagcgcg cgaagctgct
     8161 tcagcgatgg tttcctgttg tgcgatttga ttttcaagac gccggctgat gtcgcgaaag
     8221 acagcttgaa tgccgccgat cttaccttgt gcgttgcgaa gcggtatggc tgaaacttca
     8281 atgaggcgcg tcgaaccgtc ttttttgcgc caatcgaaaa ggcttcgctc ggcataaccg
     8341 cgatgcccta aaatagatag taacgcatca tggtcgccgg tattagcata tatcgtatca
     8401 acggtgaggc cgtaaaattc actcggaccg tagtatccca atatttctgc ggccgactga
     8461 ttggcaagaa tgatttgacc attttcgttg attacaaaaa ttccgtccgg taggctattg
     8521 ataagttcgt cgtatttatt ttgaaaatct aaattcatgg agatttattt ttttccgatc
     8581 agaacacgtt cataattttg aatatctttg taacattgct gatatgtata tccggcacta
     8641 tgcaaaatct gagctacagc ttccgcctga tcaaagccta cttccagcgc aatatatcca
     8701 aatgccgagg agggatccaa aatatccgca gcaatatggc caatcctacg ataaaaagcc
     8761 agcccattat cttcggcgcg caaagcggta accggttcat aatcacgtat ttctttatcc
     8821 aaaagagcgt actccttatc agaaatgtac ggcggattgg atacgatgat atcaaaacga
     8881 tgtttcaaac tttcgatcga agatggatcg aaaatgtcac catgttgcca ctgaatatga
     8941 aagcccggta aaagtttatt cgcgttttct tgtgctacga ccaatgcgtt ttgacttatg
     9001 tcaacagctg tagcttgtaa ttcaaaaccg cgttttttca aataatatgc aagactgatg
     9061 atgattgctc cacttccggt tccgatatcc agaatgcgaa gtgattttat attttcactt
     9121 tttccgcata aatccaggac gcgttcaact aaaagttcgg tatcgggtct tgggattaac
     9181 acatttcgat tgacatgaaa tgtcaatccc ataaaatccg tatgtccgac gatatactga
     9241 agcggttcgg caagcaatcg tcgttttagc gaagccttaa attcatcgcg ttcattttga
     9301 tccaacgggc gatcataatt gacatacaaa tccatacgtt tacaacccag cgtatggcat
     9361 aaaagccact ctacgttggt acgtgcggat tcgatgtttt tttcactaag ataatgtata
     9421 gtccaatcca tgatggattt gactgtccat atctcaggca cggcagaact catgatttat
     9481 acggtttccg atcggtcttt ttcgtcttta gagataatat ctgtgacgtg gtacaccaag
     9541 gttccggatt cgcttacttg taattgggcc atgccgatgc ggcataaatt ttccaataaa
     9601 gtttgggcat ccgaaacagc gatatccagt tccgtagcgg tttccacgat agtcagttta
     9661 ccgttaagtt tttgtgccaa ctcaagcaga cggtcttgga tatacacttc gttgtgtttt
     9721 ttctcgaggc gatttatttt gcgcagttgt acgatcagca gaaaagtaag tatgcctgga
     9781 acgataccca tacaaatgcc cgctccgatg agtgcgccca gttccgttgg tttttgggaa
     9841 tgcggattat cagacagtgc gactaaagaa tcgttttgga gtgcggataa tttagattgt
     9901 tcagttgtag tatagtttcc cgtaatcaga tcggacatca gtgcgggtat aaaaatagcc
     9961 gcgcttatcg tgaaggggat gataccaacc caacacaata tcttttgcat aagaaaggag
    10021 cgatgggcga cgggcataga gattaataga atttatgaaa atgatgtaca ggcccatgtc
    10081 cgctgcctac agaaaaagcg tgggttatgg ccattgaaat atatcgtttc gctaaacgta
    10141 cagattccag aaccgtgaag cctttagcca aatgtgcggc gattgctgcg ctgaaggtgc
    10201 acccggtacc gtgagtattt ttgcttcgaa tacgttggtg tcgcaaatgg taaattttgc
    10261 gtccatcgta aaaaacatca atagcttcac ctttgaggtg accgcccttt actatgatgt
    10321 acttaggacc catcgcgcgc agcgtgtctg cggctatctc catatctgaa attgttgtta
    10381 ttttctttcc ggtgagcact tccgcttcgg gaatattagg cgttaggacc gtagcctgcg
    10441 gaagaagtcg ttcgataagt gcacttatgg cgttgtcttg caataaacga tcaccgcttt
    10501 tagcgaccat tacaggatct acgactaatt tttttacgcc atacttacta agactatcgc
    10561 tgacggtttg aattatatcg gcggtggaca acattccggt tttgacggct tggggtttta
    10621 tgtcggtgat cacggcttcg atttgccggg aaatcacatc tgtagaaaca tccatgatcg
    10681 cggttacacc cacggtattt tgagccgtga tcgctgtgat gaccgatgcg ccgtaaacac
    10741 cgagcgccga aaatgttttg agatcggcct gaatacctgc accgccgcct gagtcagaac
    10801 cggcgatagt caatgcttta cggggattca tctgtggtga ggcgtgttaa gaagttgcgg
    10861 tttccggagt cggctcctga gccggtctgc ggcaatcgta ttctttaccg ttgtatgtaa
    10921 atacatcaag ttggtaatct ttgagttcac gtcgcgcata atcctgtgcc ataccggtcg
    10981 taataaagta atcaacaata tccggatcca agtggccgtc cttgaccatg aagccgagaa
    11041 ttttgataca ttcagaaatt ttctttccgg gtttatacgg gcgatcggcg gctgttaagg
    11101 cttcaaatac gtcggcgacg gccataatac gttgttgtgt attgagttgt tctgccgtaa
    11161 gtccgtacgg ataaccttta ccattaagcg cttcgtggtg cgcgccggcc cattcggtaa
    11221 cacggctaag cttattgggc catggcaatt gctgaagcat tttgatcgtg agaacgacgt
    11281 gatcctggat ttttttacgt tcttcttccg tcagcgtacc gcggcggatg gagagattat
    11341 acacttcatt ttcggtgagc agttgcattt cctgcccttc cattatgatt ttctgctgag
    11401 cgatattacg cagacgctcc agtttggcgt cctccataaa ttcaccgccg atattacata
    11461 ctttgataaa tgcggcgtcg tcttctattt gttttttctt ttgtgcgtag ctttcttcta
    11521 aggagcttaa ttttttcttt ttatcgcgcg ctttggaagt ttctattttt gctcgctcac
    11581 gaatataacg aacttcttcc tcgcgtttaa gcacttcaaa cttagccatc accgtgtgaa
    11641 tacgatcgta aatagtttct agttttgtgg atttatcaac gacatattcc ggcgtggtaa
    11701 ttttgccgat atcgtgcatc cagcctgcga tacggagctc gttgtgttca tcttcgttca
    11761 tcgtgaaatc tttgtacttc ccttcggaag tatcgctcat agaacgcgcc atagcggaag
    11821 taagttccgc tacacgacgg atgtggccgc cggtataagg tgatttttcg tcgattgcgg
    11881 ccgcgataac ttggataaac gaatctaaaa gggcacggag gtcgttgata agcgtggtat
    11941 tggtgatggc taccgccgct tgcgaagcaa gcgattcgat cagttcctga taatccacag
    12001 agaaaggaat cgtcgtacca tcgtgcgctt tggcattgag caactgaacg acgccgatga
    12061 tctcattttc gtgattgcgc attgcaatca ccatcatgga tttggagcga tacccggtgt
    12121 tcttatcaaa cgcttttgtt ccttgaaaat cgaaaccttc gacatcgtac acatccggta
    12181 tattgacggt attacctgtc agcgcaacat aagccgaaac ttgttgttca ttgcgtgtgc
    12241 catcgtcttt gatcagtttt accggaggaa aaggaatggt ttttccgctc gtaccaccca
    12301 tggcgaagtt gagagacttg gtacgcatga tcttaaaagc gataccgcgg tcatcgggag
    12361 tttttatata caaggtgccg ccatccgcgt tggtaaagct catcgcttca acgacgatca
    12421 tttccaaaag tgcgtcgagg tttcgttccg atgatagcgc cgatccgatg cgggtaagtt
    12481 tcttctcatg taccaattga ttttcggtaa aactacgaac caatccaata atacgatcag
    12541 tccggtcagc cgacaaatcc gtcaaatgcg acacgatttc ttccaataat ttttggacaa
    12601 tcgggtggtc tgagtatttc atagtggttg tgatttcctg cgcgctcata atctctctcg
    12661 atacggtaaa accgtggtct ttaatgatca acaaagatca acgggaacgg tacgcattcc
    12721 atgatttgtg aatcacggcg gccaaagtac atttgataaa atcaccggca ataaaaggta
    12781 aaaatcctaa cgaaaaagcc gtggccatgc cctgaccggt ataaagtttg agttgtaaag
    12841 tgccgatcaa aagaacaggt aaactggcga ggaaaaaggc gccgatattg taaagcaaat
    12901 tggattcttt cataacaagc ataccggtaa gaaatgccat gatcggaaaa gccagcaagt
    12961 aaccgccggt gggtccgata agatatgcga tcgtggatgc gccgccggca aaaacgggca
    13021 aaccgcagat accgtagaat aaataagccg cctgactcaa agcgccacga cgagcaccta
    13081 acaaagcgcc ggacaaagat accataagcg tttgcagtgt gatcggtacc ggtgtaaaaa
    13141 ataacggtac ttcaacccac gctgaaaccg ctgtaagtat ggaaaaaccg atgaccaaaa
    13201 gaaaatcttt gagcattcct gcgtcaacgg ctatggcttt ggatttggag ttcatatacg
    13261 tgatcaatcc ttacattaaa aatatgttaa cagatagggt ttgataacct cttatgtgag
    13321 aacggtcaaa tgtacatccg tgattgacct accgcaagtt atttttaatc ttaattttgc
    13381 gaaggtgaat cactgacaac agcctcctca atacgcgctt catcaacggc aggggaggta
    13441 ctattggtat atcgtacgac gcggcgctcg cgaatcacaa caaccttaat ctgaccgggg
    13501 tattgcattt cgttctgaat tttttgtgcg acattggagc tcagcatggc cgactgggca
    13561 tcatccattt tttcggcttc gacgacgatg cgaatttcgc gtccggcgaa aatagcatat
    13621 actttagaaa cgccgtcaaa ttgttgagcg atttgttcca atttttcgat ccgtgcgcca
    13681 tacgcttcca gcggttcacg gcgcgcgccg gggcgagaac cggaaataat gtcggctgcc
    13741 gtaacaagtt gtgaaatcgg gtggatcggt tcggcttcgt catgatgtgc gagtaccgag
    13801 ttaatgacga cctcgtgctc tttgcaacgc gtcgtaactt cgacaccgat cgtgacgtga
    13861 cttccttcat tttcgttgct ggtggcttta ccgatatcat gaaacaaacc tgcgcggcgc
    13921 gcaagctgaa catcatagcc gagttcagat gccatattac cggctagcag ggcgacttct
    13981 ttgctgtgtt gtaaaacatt ttgaccatag ctggtgcggt acttcagacg gcccagcatt
    14041 tgtttcattt ccggtgcgac atttttaatg tttagcgaat tgagcacttc atcggaagct
    14101 ttattcattg ctgtttcgac ttgttttttg caacgctcga caacgtcttc aataaactcg
    14161 ggctggatat tgggcgtctc cgtgaggcgc aacattgcta atctggcgat ttcgcgtttc
    14221 acaggatcca tcgaagaaag aacaagcact tctggggtat cgtcaataat gaccttcacg
    14281 cccgtgattt gttcaaacgc tttgatattt cgcccttcac gcccgatgat cgcgcctttg
    14341 atcttttcat tgggtaaatc caccgttgtg agagttatct cggccgtttg atcggaagct
    14401 aaacgctgga tggcgtccga tataatccat ttggcctcga aacttgcctt ctgcgtagct
    14461 tcgtttttca tctggagcat cagttcggcg gcttcttttt gagcccgttg cgcaacactt
    14521 tccaaaaact gttttttcgc ttcttcgtag gacaacttgg taatattctc taacttttcc
    14581 atctgctgat ggatgaccga ttcgacatgt tgggcacgcg tctggagttg ctcttgttgc
    14641 gtcgtaagat ctttttgcga acgttggagc agttgttctt tttcgacagt accgctcatt
    14701 ttgttgcgga tttcgcgttc gcggttaacc agtttttgct cgtactgttt aagacgtttt
    14761 tcacgttcgt ccgtttcact gacgattttc attttgatct gatgccaacg ttgtttggat
    14821 ttgacttcag cttcgttgcg tatggtttcg gcttcacgtt tggcttcgcg tatggtgttt
    14881 tcggcgattt tgcgaatttc cgatactttg ttttttccga aataagttgc aaagacccat
    14941 ccgagaataa acatcacaat gcccgcggcg cacatgatga taacagataa actatccata
    15001 actatcctgt aagtttctta agttttaact atgtctatag tcattagtat agacgtaaaa
    15061 aaccgcgcat aagcggtttt cattacgata aagaatttag aattttgtga ttcaaatagc
    15121 aaggaaagaa caatgaatat atggtgccgt ggccgggagt taaactttgt atgatgtgta
    15181 aagcgaggat gaaattacat ttaattatac tcagtatagc gaggaacaga aaaactaaat
    15241 cgcgaacctt tgcctttttc gctggttacc catacgcgtc cgccgtgcaa ttcgattact
    15301 tttttgacga tcgccagacc aagccctgtg ctgacttcac cggccgtcgg acgtgcgctg
    15361 agtttctgat acgatttgaa tactttgtta aggtcttctt gtgagaggcc ttgtcctgta
    15421 tcttcgacat gaacgcagat ttcgcgatct gttggttcac aaaaaacgcg cactgcacct
    15481 ccggggtgcg tgtatttgat cgcattggag agcaaattat ccatcacttc caaaatccgg
    15541 ttgcgatcta caaatacatt aggcatgagt aaaggccgct cgacagctaa tcgaatgttt
    15601 ttttgttcag cagcacgacg atgcagattt tcacattcgt ctatgatgcg ctgcaaactt
    15661 tcactgtgtt tttctaagtt gactttaccg gcttcgatgg agctccaatc caataaagta
    15721 gaaacaagac ttgccatttg ttgtgatact ttgtgcacag tatctaaatc attctccaca
    15781 tctttcatcg tagcgtgtcc atgccggata tcattgatca tcagttgtac atagccgatg
    15841 attgttgtca gagggtttcg gagatcgtgt gccgcgatcc cgaggaattc atttttcttt
    15901 tgattgagga cttccagttc ttcgtttttt aattttaatt ccgtcgtacg ctcgttgatg
    15961 attttttcaa ggcgacggtt gtattgctcc actctttgaa ggcgcagccg atgaaacgac
    16021 cacaacgcca aggtgatcaa aacaacgatg gccaaacgaa aataccaagt tttccagaat
    16081 ggcggtaaaa catgcaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
    16201 aatatccatc aaacttatac cattgccgtc cgtaccgatc caaagatgcg gatgagccgt
    16261 atccggatcc aatgcgatgg tcataatttc atttcccgaa atgctatttt tatatgtcgg
    16321 gtttccttta aaatgtttcc aattgttggt ttcaggttca aatctgttga gtccgccgcc
    16381 ataggtgccg atccacaaac gatttacatg atcagataca atggtacgaa taaaattgtg
    16441 acttaaactg ttcggattac cgttttgtgc aacccaacgt ttaaaacgat tattcgattc
    16501 gtcccaaaga tgtaatcctc catacgtacc aatccatagt cggcgaacac cgtttatgga
    16561 ttgtgcataa atggttcgta cacgattatc gttcaatgag gtggagtctc ccggtcggct
    16621 tacgattttt ttaagaacac ctctctgtaa atcaaaccaa atcaaacctt cgccgaatgt
    16681 gccgatccag taaaaaccgg ctgggtcttt gtagatagaa gtgatccata ccgacgaagt
    16741 gttatcttcc gtaggttcaa gataggttgt aaacgatcgt ttcttttcat tgtacacgat
    16801 caaaccgcgg tccgtgccgg ccagtactaa ttttccattg gcgctatgat cttcaaatac
    16861 agcggcgacc cgtgtgcttc ggagcgtgtt gttttggata tcgtcagcga cgatggaaaa
    16921 ttttggtggc ttactatcta ataaactgac gccttcaccg cgtgtcgcga tccatactat
    16981 tccggagcga tcttccataa tccaacgtac atcattatgt cccaacgaat gggcataatt
    17041 cggatcgtga tggtaagttt tgataaccgt cccatcgcta tccataataa acattccgtg
    17101 ctgaatggta ccggcggcca gatgaccgtt ttgtaaacgt gtgacgacgt taacgcgttc
    17161 gtgtgttaaa cttcgtcgta ctgatgccgt gcgtagatcc acgcggtcca aaccgtcttg
    17221 ggtaccaacc cacaaaagac tgtctccatc cgcaaataac gcacgaatag tgccgtgact
    17281 caatacaaga ccggggtaaa ttttttcgat gcgttccgtt tttaatccgt caggtgtttc
    17341 cgacacgcgg caaagaccgt taccggtgcc aacccacacg gcgtgatttt gttcagccaa
    17401 tgaccacaca cgcggatgcg gtaatgacgc ttcgctatgc gggtcgtgcg acacgtgtac
    17461 gaatttttca gttaccggat caaaaatatt aactccaccg gaattggtac cgatccatag
    17521 tttgccatcg gagcgacgta agatgtattg tatgcgtagg tcactcaggg tatgatttag
    17581 accggctatc ggtttgtatg ttttaaatgt agaggtggtt cggtcaaatc gcgctaaacc
    17641 accgccccaa gtaccaagcc ataaacgcgc ttcgtcatct tcgacgatgg aagtcacatt
    17701 gttattgggc agcgtggtcg aatcacgcgg atcgtgttta aaatgaataa atctatagcc
    17761 gtcataggcg ttgagcccgt tttcagtacc catccagatg aagccgagcc ggtcctgata
    17821 gaccgtataa atggtgctgt gggataaacc gttttcgacc gtgagttgac taaaatgatc
    17881 aggtaaggat tgtgcgtacg tattctgcgg tgcgggatta agccaataac ccgtagcaaa
    17941 aataaggaga gatttgatga tgacaccgaa ccgaatcatg aactcccttt tgtagcaagg
    18001 atgaccaatg gcaatcggaa cttcggctat attttataat aagaaccata ccgggacgga
    18061 ttacagtccc ggtatgaata tagacatact ttacagtgga tgcaatatac tacaaaccga
    18121 gcaggcgttc cacaaacttc gtatcaaaat ctccgctcac gaaattttcg ttgagcatca
    18181 tccttttctg aaaggggatc gtagttttga tgccttcgat gataaattcg tccaatgccc
    18241 cgcacatctt gctgatggct tcggtacggt tttctcccaa aacgatcagt ttaccgatca
    18301 tcgagtcata gttggacggg atttgatatt cggcatacgc atgcgaatca acacgaatac
    18361 cgtaaccgct cggaatatga aacgcatgta tttttccggc tgacggacga aaattgtgat
    18421 cgggatcttc ggcattaatg cggcattcga tcgcatgtcc gcgcggtttg aaatccgatt
    18481 gctggaacgg cagtttttct ccggcggcaa cgcggatctg ccatttgatc aggtcaatgc
    18541 cgtataccat ttccgtgacg caatgctcga cctgaatacg cgtattcatt tccataaagt
    18601 aaaaattctt ttctttatcc atcaaaaatt cgatagtgcc cacgctttcg tagtttacgc
    18661 tttttgcgcc acgtacagcg gcctcaccta ttttttgacg caaagcttca tccaccgcgg
    18721 gcgaagggga ttcttcgatc agtttctgtt gacgacgttg catcgaacag tcgcgttcgc
    18781 cgatatgaaa tacgttacca tgctgatcgc cgatgagctg gatttcgatg tggcgcggtt
    18841 tttcaatgaa tttttcgatg taaacatccg ggttcccaaa tgccgattcg gcttccgccc
    18901 gtgccgtgtt atacgcattt tcaaactccg attcctcgcg aacaatacgc attccgcgtc
    18961 cgccgccgcc ggctgtggct ttgatgatca cgggaagccc ggcttctttc atgatttcgc
    19021 gggcatgttt gatatcccgc acgataccgt cgcttccggg gacaaccggt acgccggctt
    19081 ttttcatcgt atctttggca aaagccttat cgcccatttt ttgaatcatg tgagatttgg
    19141 ggccgataaa tttgacaccg accgattcac agatttccgc aaacttgatg ttctcagata
    19201 aaaacccgta acccgggtga atgccgtcgg caccggttat ttctgcggcg ctcatgatct
    19261 gtggaatatt cagataactt ttggcacttg gcgccgggcc cacgcaaatg gcttcatcgg
    19321 caaaacgcac gtggagtgaa ttggcatccg cttcggaata tacggccacc gttttgatac
    19381 ccatttcacg gcatgcgcga attacgcgca acgctatttc accgcgattg gcgatcagaa
    19441 tttttttgaa catacttcct tccggttaat gatacaagcc ggcgcacaag cgcccgtatg
    19501 tgttatataa tacgtattat gatcgtttat caatcagata attgctgccg tcctttttct
    19561 tcaccgcttt tttgacttcg gcagcgattt gggcgatctg tcccatacta ctcaactcgc
    19621 ggttttcgtt gcttaccacg gcaatcgaaa tagacataag cggaaattga tccatttcgt
    19681 cttttctgtt tttagttata atatggcccg ctttacggtc tgtttcgtca tacagggacg
    19741 gataaaaatc atctaatcgt tcgataatcg ttttacaaat cgtttccact cgatcgtaac
    19801 gcgaaacgat gaagacgaag tcatccccgc caacatggcc gacaaaatct tcttttgcgc
    19861 ccagttcgcc gatcacatcg aggattatgc gcgccgtacc ctgaatggcc tcgtctccgc
    19921 ttttgaaacc gtatttatcg ttgtatgctt tgaaattatc caaatccgca taacaaacgg
    19981 caaaaggtgt tttttgtttt tgattttgtg ctattctaaa attgatgtcg tcttcgatcg
    20041 cataatttcc cggtaatttg gtcaggggat ttgcgccgat gctttcttta taacgacgga
    20101 tcaaagcttc taaacgggcc gttaattcct gatgatccac gtctttggtg atatagtcat
    20161 cggctccgtt tcgtaaggat gctaatttgt cgggcaactg atcgctggag ctaaacatca
    20221 caatcgggac attgcgtgtg gacggacgct ccttgagttt ttcgcgaacg gtgtgcccgt
    20281 tgatgtccgg catattgata tccaaaagaa tcaaatccgg ccgtatgcgg gtcatgattt
    20341 ccaagccttc gcgtccgctt tcggaggtga tcacttcaaa acctttgttt tggagtatcg
    20401 tcgaaaagat aaaaatttta tccggttcat catcaatcat caatatcttt tcgcgaaaat
    20461 ccatactgct tcccatagta atccgttaac cgatgaaaac gagcgaaatt atcaaaacca
    20521 cagacgaaag tcaatcaagg agttcaaccg cataatgctc aaaaaacgca ttattgggac
    20581 acggcgtcat tgataaaatc acacaaaggc ttcatggctt taaacccttt gatcacatgt
    20641 tcttcaaaac gcgggttgga aagctcttta tcggatattt tatgcaacac cacatagctc
    20701 gtgtatttga gccattcggc atgcgggtgc gaagccggaa accctttggg catggttttc
    20761 agcttttccg gttggagttc gttaaaatat tttttgaatg ccggtgcctt gatgattttt
    20821 ttcaagccgt cggcttcttc cgccacatag ttacgcattt tgagaagaat atccgatggc
    20881 ggcatgtaca aacccccacc cagcatggag gcgccgggtt cgatgtgaat ataatatccc
    20941 gctccgctgg atttacgtcc gcctgatgca aggaaagcgc caaaattggt tttgtagggt
    21001 gttttgtctt tggaaaaacg tgtatcacgg aatattctga atatacagtc tttgggttgc
    21061 aaagaagcca acgacggatc cactacagtc atatcgccga tcagatcgcc tacgaaattt
    21121 tcaaacgaca ttttggccat agcataatca tcgcgatggg catcaaacca tgtttttttg
    21181 ttattccttt tcagtttttt taaaaagccg agaatcgtgc cggtttccac agaagacctc
    21241 ctcgcttaga tagttggatg aaataaaacg gtatgcgtaa agtagtttgt gaaaaaacta
    21301 tattccattg tttttaaaat agattaataa accgcttgat tataaacgtg ccggtgcata
    21361 tcatgttttt gtctttttga tttcaaattg aattctatca ttattaccca attacattgt
    21421 tatgtctgaa gtattctccg ggatattgga aacggatccc aaaggttttg gatttattcg
    21481 cacgctcagt ttgacgctat cgccgtcacc gaaagatgtt tttgtgcctc ccggaataat
    21541 cacacaaaac cgtctgcgcc ccggtgttct cgtcgaagga acaggagcgc ccggcgacaa
    21601 aggcaataca cgtgtcgcta cgattacgcg tatcaacggc atggaacccg aagcatggaa
    21661 atcggtgcag gatttcggaa ctttgcaagt gatccaaccc agggatcgga ttagtctgac
    21721 ggggcacgaa caaaacacct ccatgcgtgt ggtggatctt ttttgcccga ttggacgcgg
    21781 tcagagaggg ttgatcgtag cgccgcccaa agcaggtaag acaagattgc ttcaggaatt
    21841 ggcgcacgcc atttcgatga ataacgacga catcactatg atcgttttgc tgatcgatga
    21901 acg
//
LOCUS       DAOQGF010000050        21854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1097_length_21854_cov_6.793110, whole genome shotgun sequence.
ACCESSION   DAOQGF010000050 DAOQGF010000000
VERSION     DAOQGF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1097_length_21854_cov_6.793110"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5339
                     /locus_tag="PK937_08510"
     CDS             <1..5339
                     /locus_tag="PK937_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH32651.1"
                     /translation="AKVVGSGNAGGFRYVQESGNTSHNNSWRLSGLSDGTYYWSVEAI
                     DQVYRNSKFQSAAQFTIDGAPSAPDSLSVTSGVNSVTLMWGSTNKSDVSKYFIYVSAD
                     NEGYTLVDSTTTGIFGTTKTVTKGPSGGILQNGSIYYFRVEAMDHNGYVGPYSNYVTA
                     IPSATPGKWFVVNTDDSGPGSLRSAIDSTNLSTQKDTVVFLIPIGSSITLNSELPAIS
                     TDYTVIDADSNDDGKPDVSLSPIGEGGGFFYGLTIQSSNNLIQGLVIGGFGDYYSGGG
                     SLFINGDNNRILGNYIGVSSDGMSTHGNNHGILINGGSSGNWIGDGTGTGRNIIGGNQ
                     QYGIYLNGASTSVNNTKILGNYIGIATDGLTALPNSGPGISIYNRALNTIIGNGTAEG
                     RNIISGNSSDGILLTDNNGHIEGTSILGNYIGTDVTGSVGVGNNNHGIFFNTLTGEGA
                     SIVNNTIGDGTAGGRNVISGNAGSGINFYYYQVHDNTIKGNYIGITADGSAALGNGMY
                     GVNIEHSNNNIVSSNTISGNAYDGIMINGSYGEANNNVVTGNYVGTNPTGSFAIANGQ
                     RGIYLQNGASYNQIGGTAAGEGNIVSGNLNGGIGFGAAYTPTVSNQILGNKIGTDATG
                     MFGIPNKESGIYINGSNHNYNYIGDGTTGGRNIISGNDGDGIYIYGSASDNFILGNYI
                     GVDATGNTGLANDSNGIAIYNSGHILIGDETAGGRNIISGNGKEGIAIYAQSTEIVIG
                     DGGNKALVNDAGGRVAKPELPGANNIDIRGNYIGVGTNGSTSLGNGGAGIEIEAVFEG
                     FYSQYDSITSNLIAYNTNGIELVGAGVSSTYIHNNTIYGNTADGIAIEEGAQNGVTTP
                     AITSISDFVVSGTASVGAMVYVYQDAGNQGRYYLGSSAADGEGSWSYNGLYLPGYNVT
                     ATQFLDEQSSAFSAPFATPSGTIHATTTSLSYGNVLAGDSATLTTRIYAIGNPVVMNS
                     GSLVLGTNAQMLATLGTDTLFMTDTITVQVRFKPVNFGFLTDTLRIYYGEGNMFDIRI
                     GGTGTSGTIAASSIGFAMETLNGDSTSVPIQMYTTSGWVQLNSATFGQNNYFTYSIDK
                     TLPYIMKPGDTAGVTVTFKPTTFTNSADTLYFANNSSDGILELMFSGLPLPGTVATNL
                     ASQDFGNVLLGDSLELTTKIYAPSGRVEITSVTLSQGDVLSMTVTPALPFTLNAGDTI
                     LVKTKFIPSDFNSFNDTITVLSNASNGTVTVSLSGQSLPGTISSAASAFDFGNVLVGD
                     SAQTSTRYYATGGPVQITGSSLVTSGETSVSFTPSLPYLLHVGDSVVVTAKYKPLTFA
                     AMSADTIHLISNASNSGKLSIPMSGFGQTGTLTFNTTPANFGSIRVADSSTAQIVKVY
                     SSVGQIPLTGYTIGSHFKATTSTVFPITIKSGDTVTLAVKFKPQTTGALLDTIKIGNV
                     RIPLAGTGLENVAPTLTLGVTSVIALKQYLGIYMYSSEALSARSAAFKFNDVSVTAPT
                     FDVVSGVNNLYYAQYKLSAAGSLKIDATGTDIAGNIGTRSKTYTIGSLTKDQTLAVEA
                     EGLRISGAKGAIGESGFMIITKSQTYDGDPVGLAKGTSKSATDFANANIQSWDIISTV
                     ALEKSVTLTAVYDETWIHALQTQYANFDERKIGFYREENGSLVYLGGEGRNGNVALQT
                     KSLGKFVIQYNDEHRYLPSEITLAQNYPNPFNPTTTIEFGLPEASHVKLVVYNILGQR
                     VAELFNGQRNEGYHKFVWNGRNAQGATVASGVYLYRLETNKGNVTKKMMFIK"
     gene            5382..5942
                     /locus_tag="PK937_08515"
     CDS             5382..5942
                     /locus_tag="PK937_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32652.1"
                     /translation="MKHFKVWLAGALMIALSAGCSEDPATDPLGDAWQLFINGQYAEA
                     QTSFEALIETQPGQAYAGLGWTAMKLDNIPASVDYFEQAAGDSLVHGYAGWAIASWAN
                     KDFTHIVERAGFVIRKEPAFEFEFYKDITISTMRLMQAYGYYHTGNMSACLARIQELD
                     GGFASTTDPALLLAKLDALSATASGL"
     gene            complement(6028..7845)
                     /locus_tag="PK937_08520"
     CDS             complement(6028..7845)
                     /locus_tag="PK937_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNH32653.1"
                     /translation="MRWFIFFFTTTLFAQTATERIASVSIHGNRIVNDKTLLSLLYHK
                     PGSVFDHEQSRNDAMRILEYYRDRGYLASSIDSIQQKSAEGTDGIRLIFFLSEEKQAK
                     FGSVRISDESDISASQITEVMRWKKGDMFSPGQWTASVNRMLDWFENNGAPFVHIALD
                     SVWMRRDAKSDFIIDILLHIKRRIVKPVGEFIVRGNESTKPLTLTREMRMKLPRGYDH
                     RAIQDGLTRIRRFPFIRSAELTSVTMRPDSLLTVWLDVEEDRANTMDGIIGYVPRDAA
                     RDEKGYFTGLIHVSFYNLFGTARQLSMHWQKKNRLSQDLMVQYTEPWLVGWPVNGRIA
                     VQQQIEDSLYSRSAWEMELQTEITRDWSVLAGVRLQSVEPADPKTAFIFNTPQSDGYA
                     WNGGLTGDMRDEQINPRNGLYYHTVFSYGRTREKNWTLSSDGTDTITYGGEQKIIRSF
                     SQSIRTQTWRMDLEIYREPVHRWVIANGLHFSVFRPGRDVAPLSEQFLFGGLKTLRGY
                     TEGFFSGTRVGWNNLELRYVTGRASRLFVFYDQGYYYRRAYADLERTKINKQDGFPWG
                     YGFGMRFGTRLGQFALDYGLGQNDRPSDGKIHFGFTGTF"
     gene            complement(8275..8886)
                     /locus_tag="PK937_08525"
     CDS             complement(8275..8886)
                     /locus_tag="PK937_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNH32654.1"
                     /translation="MTPDEYYTIAESCTSEIKVKGSKFIGYAAPVQDKDGAEQFLQSI
                     RKNYFDATHHCFAYQLGLTPHVSVRLSDDGEPSGTAGKPILQAITAKNWTDIIVIVVR
                     YFGGTKLGTGGLVRAYGDSAADVLSRCCAEQRLIYENLRIQFPYDEINTIMRCVHHAG
                     GKAGETAYSEQATMDVAIRKNEVLHFCDAITHQTRGRATVIPL"
     gene            complement(8904..10205)
                     /locus_tag="PK937_08530"
     CDS             complement(8904..10205)
                     /locus_tag="PK937_08530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNH32655.1"
                     /translation="MARDVKAEKLPQTLLLKNVRLLDLIHKSDQVVDILIADGRYVRI
                     GKCDDPADRVIDLSGKVVTHGLMDMHVHLREPGREDKETVWTGANAAMVGGFTAIACM
                     PNTTPPIDTADTVQYVKHKAQGHLVDVHPVGCVTKGREGKELAEIGSLVEAGAVGITD
                     DGDPVYNAEIMRRALEYVKMFNIPVIEHAEDKQLSNRGSMNEGFTSTRLGLRGIPSIS
                     EEVIVARDILLSEYTDAPVHIAHISTGGTVRLVRQAKQRGLKVTCEVCPHHFTLTDEA
                     VENYNTFAKMNPPLRTSADIEEIKLGLQDGTIDIIATDHAPHTIEDKECTMDEASFGI
                     TGLETCVGITLNDLVHKSVISLESALYKLSIAPRKLLHLPLPEFIIGAQANLTILDTE
                     MRWKYDVHQTCSKSINTPYHDYAMKGKAVGVIHNGKIFYAS"
     gene            complement(10207..10602)
                     /locus_tag="PK937_08535"
     CDS             complement(10207..10602)
                     /locus_tag="PK937_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32656.1"
                     /translation="MSQRLLWVMLFGWMMFVGCSHNYYMRSDEPKDETEKEKRRKEIE
                     SAFMYKIKPGYVIQIRTTNGDIHRGVFYSYMDGIVSMRIYDSFRDFELSDITGIKYDV
                     QGGEMKALVFSLILVTMIYIIYEFSQEKH"
     gene            complement(10620..11021)
                     /locus_tag="PK937_08540"
     CDS             complement(10620..11021)
                     /locus_tag="PK937_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNH32657.1"
                     /translation="MDQDKLAVIGTLYSVIGEEGLRKLVDRFYDIMDTDSSAREIRAM
                     HPTDLTESRDKLFMFLVGRFGGPPVYMEKRGSPALRMRHAPFPINGNARDQWMACMGQ
                     AMREQLHEPGMIDLLNEFFYMVADAMRNQPH"
     gene            complement(11028..12767)
                     /locus_tag="PK937_08545"
     CDS             complement(11028..12767)
                     /locus_tag="PK937_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32658.1"
                     /translation="MKRMMPLLLMILATVVWSGCFETKADYTINPDGSGKVTVEVTTA
                     GMDGMMGNMSGKTPDGSKTARDIIQQATGVDTWKDVEFRVLDDGRNYFKGTAYFKNIS
                     NVKFKNAGAGDSIVVYQDAQKNWVLELATNPSKKSDKAKSGSEKIPTTDAEITKAVKE
                     QKNGYNQMRPMLLAIMGTMKSEMNFRLPGKAQSSNNMTQQADGSYQVSFTGEKFIQVL
                     DKLFADENWLKEQARAGANIKDNPPSGDAVNELLFGQKGPVRAVVSGKTAPQFNYEAE
                     VSAAKAGYDAMIERLGLNQTESSATMESSKPERAVQSYQGGTFDWVKITRVTYSNVED
                     PDYLQFGTDQSYQVTFTGALPGAIVKTERAVVTAAIADNGQNLLHESEWYRETNYVEM
                     SKQKDFLRWTLTLTVPDATAKGIKRIEGTVYAMRSTSTSVVDPGIRKFEKGAEGNQYG
                     AVIEDIQDDEYTHQMVIRVGLPDFKLNKMDILDEKGNPVGISDRYISTYTEESTTMVL
                     NLNTAVPKRGTINIHIHDDVEGVELPFVVENVNFLGQSMSPAVEQKQNKPQEKDLPRD
                     PVKEKTKGKGKKK"
     gene            complement(12813..14015)
                     /locus_tag="PK937_08550"
     CDS             complement(12813..14015)
                     /locus_tag="PK937_08550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNH32659.1"
                     /translation="MHRIRLKSGKEKKIRNAYPWIFRDDIATQFKDRGDIPNGAIVEI
                     ADAEDKFLAVGYYNALSHIVVRVLDRKNRPIDKNFFKQRIQRAFDFRTQLALPSDARR
                     LIHAEGDRLPGLMVDQFGAYLVVQFRSLGMEKFRTEILETLVEVVKPKGIYDRSDMES
                     REEEGLEPFSGVVYGAIPEEIVISENDLLFHVDIHHGHKTGFYLDQRENRAYVRTLVK
                     PNQTGLDLFSYTGGFAVALAQSGVRMTAVDQDAAALEGGRANARRNNVEKRLQFIHAD
                     VFKYMEEQVAVKTSTPDKVKKYDVIIIDPPAIAKRKEGIEKLKWAYWKLLHQAIQLLN
                     PGGHIVLSSCAYHMPVDLMLEAARFASADNGVSMRVVNITYQPPDHPWMLQIPETLYL
                     KTVYFQLM"
     gene            14478..15494
                     /locus_tag="PK937_08555"
     CDS             14478..15494
                     /locus_tag="PK937_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32660.1"
                     /translation="MRKLMTMIALLALCSVQTYAKTWTVDQRPGANFQTIAAAVASSS
                     VLAGDTILVSGSSIAYAGFSFNKKLTIIGPGYFLGENTNLQADTNSVKISSQVTIQSG
                     AQGSIVMGLHFLNSLYVDADNVQIIRNRFQITSTYDYGIYLYAQDSTIIRQNYLTQTA
                     SWYHCITIASGNTQTMIQNNFLEYTGGDSNSSYGALSVQGTISGDISNNVIYGNVTLS
                     AGGFYFNNNILRYGKFNSTGITPHNNIGSSSQFSFWPALGNQSGVNMTSVFVSSGTSD
                     TKWQIATEGPADNNGSGGVDCGMYDNSAGTAYIPSGIPPIPSIYQFDASDLNNVTVKV
                     KSHN"
     gene            15554..18886
                     /locus_tag="PK937_08560"
     CDS             15554..18886
                     /locus_tag="PK937_08560"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNH32661.1"
                     /translation="MKFFTALIGLWLSATSLFGQSPPDVHSVEYFIDTDPGFGLGTSV
                     APSAATSGSDKTFVFSVSTTGLPSGFHTLYVRSRDNNGGWSQATAKAFYVMPGESGSF
                     ATAVQRVEYFIDTDPGFGNGIAMGSPSSGDATVTGMIDISALPGGFHTLYVRARDGFH
                     KWSVLNSRTFYKSVVYTPPTDPQVTYIEYFVDKDPGYGNGTSVPGFTASSDITHNFLV
                     DIGALSGGFHTLYIRTKDANGNWSIASTKAFYKPHNYVAPSDPQITAMEYFIDTDPGF
                     GAGTALSFTASSDVTVNGVVDITSVSGGFHTLYLRSKDANGNWSVAQAHAFYKAHTVS
                     NTSSNITAIEYNFYKSGSSTPVRTFTAFTPSTDVQTTFLADLSGLNIDSTYDMRVTAI
                     DAAGRRSIAATASTVIIERPNHAPEIKSSVADVTANEDFDSLIVASLDTVFHDSDMTT
                     VGDHLQFNASVNGSAVTSVLRGSQLVIFSSPDIFGSAEVIITATDDSSASVSDTIHVT
                     VNSVNDRPLATYDVAQANAGYTAYVQPLKNDTDIDGDALVIAAIVTMPQHGTATVVSP
                     ADTLISYTPTVSYSGIDSIQYAVSDGLGGLDTAIIYLQINGYINSAPQRNNTIADVTA
                     SEDFGVIALAPLNSVFGDNDMALNNDSLRYSVSVQPAIVALSLRHDTVVVNSIANLFG
                     IAEVIVTATDDSGATASDTLTVTVNSVNDAPFTQNDAVSTTGIQTVVAHPLNNDTDVE
                     SQTLTIAAIITSPQHGSATIPSGDTTIAYTATIGYSGNDSLTYSVSDGNGGLDTAKLV
                     ITIQAPAPLSLSTSVHQNPALAQYADVYVIADTALIGLPDVKMFLGSDSTALTMTVST
                     SRVYRGQFEFASSGAYTIRTRATAATGAQAVNFRSFTATLAKPGVTTNIASTDAQAQL
                     LVKSKAIVSPTYITVATDESMNGKAYQFAPALTLQEPSEITIRFEPSQYEDAGKIFIE
                     SETDGRIVRLQTGVYPEQRIAKAKITSLGKVRIVYDPSFTGSNTVPTNFSLKANYPNP
                     FNPSTTIAYDLAKEGLVTLTVYNVLGQKVKTLVHGHAPAGSYKLLWDATDERGKRVST
                     GVYFYRLETPGFVQTRKMLLMK"
     gene            18895..19479
                     /locus_tag="PK937_08565"
     CDS             18895..19479
                     /locus_tag="PK937_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32662.1"
                     /translation="MKNKIIFCALAIVLLSSCGPDPGTSEDYAATVAEGWTFFETGSY
                     PQAKERFRAAIAIDPAFSEAFTGLAWSQMKTDSLDEAVTVFSFGSATSDATADLWAGW
                     AFTEHALKQYTASNGRITNAIAADANWIFSHTAGLSVSDLRVLTAENHFALGSYAASL
                     SAVQQLNAAFTADVTTTAGQSALAAEIERLKGIN"
     gene            complement(19658..20416)
                     /locus_tag="PK937_08570"
     CDS             complement(19658..20416)
                     /locus_tag="PK937_08570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH32663.1"
                     /translation="MNTFKDNFSKQAEVYAKFRPTYPKELFEFLQGLTAEHKLAWDCG
                     TGNGQSAIKLTDYYEKVYASDPSQEQIKNAIPHDRIVYKVENAENPGAIADNSVDLIT
                     VAQAIHWFDFDKFYTQVKKVLKTNGIIAVWAYGIPTINEELDKIIKNFHDNIVGEFWL
                     PENRLIEKEYLTIPFPFDEIKTPDFFIKRLVTLSETLGHLRSWSATQKYIDKYNENPI
                     EHLSQKLQEHWKDIETKKEITWKLILKLGKIASR"
     gene            complement(20567..>21854)
                     /locus_tag="PK937_08575"
     CDS             complement(20567..>21854)
                     /locus_tag="PK937_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH32664.1"
                     /translation="IDTLLIKKGFDEITTLSFDQQAALIEEDTIFEFCMYKLDKGVSP
                     NDSINLNFTIKNQKNTLLTQNSNILKNGTYLKSDIFPRLGYFADTEKKIPTNSSSFAN
                     HYQSIDADLVNFEAIVSTSPQQIAITSGNLLREWLEDGKRYFHYKMDKPFKFVFGFNS
                     GEFAVLKENYKGVDLRVYYHPKHIYNIQQMMDGLKASLDYNVLHFGTYQYKQVHIIEF
                     SRSEGSYATTAGNCVPISEIRFINDARNLKEDGIDLSFYVAAHELSHQWWGNQVIPAN
                     ALGATMITESIAEYITAKIYEKKYGIQSALKFLQIQRKRYLLGRANETEQEPPLYLVN
                     PEQSYIAYGKGAIALYTLSEFIGVEKLNTALKEYLDKVKFQPPPYTTSIEMLTYLKKA
                     TPDSLQYLITDMFEKNDKEKTLLYFDKILERNNASH"
BASE COUNT         5761 a         5244 c         4807 g         6042 t
ORIGIN      
        1 tggccaaagt cgtaggcagc ggcaacgccg gaggattccg ctatgtacag gaatccggca
       61 acacaagtca taataattca tggcgcttat ccggtctatc cgatggcacc tactactgga
      121 gtgtcgaagc gattgatcaa gtttatcgca attccaaatt tcaatcggcg gctcaattca
      181 cgatcgacgg agctccttct gcgccggaca gtcttagcgt tacttccggt gtcaattccg
      241 ttactttgat gtggggttca acaaacaagt cggatgtaag caaatacttc atttatgtca
      301 gcgcggataa cgaaggctat acacttgttg actctaccac gaccggaatt tttggtacga
      361 ccaaaacggt gaccaaaggt ccttcgggcg gcatacttca aaacggttcc atctattatt
      421 tccgcgtcga agccatggat cacaatggtt atgtaggccc gtattcaaac tatgtaacag
      481 cgataccatc cgccacaccc ggtaagtggt ttgttgtaaa tacagatgac tccggtcccg
      541 gatcattacg ttcagctatt gattcgacca atctttcaac acagaaagat accgttgtgt
      601 tcctcatacc catcggttcc agtattacat taaattcaga acttcccgcg atctcgacag
      661 actataccgt gatcgatgcg gacagtaacg acgacggtaa gccggatgta tcactctcac
      721 cgattggcga aggcggcgga tttttctacg gattaactat tcaatcttcc aacaacctta
      781 tccaaggact cgtaatcggc ggtttcggcg attattattc gggcggagga tcactcttta
      841 tcaacggaga caataaccgg attcttggta actatatcgg cgtttcatcc gacggtatgt
      901 caactcatgg taacaaccat ggcattctga tcaatggcgg ctccagcggt aattggatag
      961 gcgacggcac aggaaccgga agaaatataa ttggaggaaa tcaacaatac ggaatttatc
     1021 tcaacggcgc ttccacgtcc gtgaataata cgaagattct cggcaattat atcggtatcg
     1081 ctacggacgg tttaacggcg cttcccaatt cgggtcccgg tatatccatc tataacagag
     1141 cgcttaatac aataatcggt aacggcacgg ccgaaggtcg caatattatc tcaggaaata
     1201 gcagcgacgg tatattgttg accgacaaca acggacacat tgaaggaact tcgatactgg
     1261 gcaactatat cggtacggat gttaccggca gtgtcggcgt gggcaacaat aatcacggta
     1321 tcttttttaa cactctaacc ggcgaaggcg cctccatcgt aaataacacc atcggtgatg
     1381 gtacggccgg cggacgcaat gttatctccg gaaacgccgg tagcggtatt aatttttact
     1441 actaccaagt acatgataat actatcaaag gaaactatat tggcataacg gccgacggct
     1501 ctgcggctct tggcaacggg atgtacggtg tgaatattga acacagcaat aataatatcg
     1561 tatcctccaa tacaatatca ggaaacgcct atgacggtat catgatcaat ggctcgtatg
     1621 gtgaagctaa caacaatgtg gtaaccggta attacgtagg aaccaaccct acaggatcat
     1681 ttgccatagc taacggtcaa cgcggtatct accttcaaaa cggtgcttct tataaccaaa
     1741 tcggtggtac ggcagccggc gaagggaata tcgtttccgg taacctcaac ggtggtatag
     1801 gatttggtgc ggcttataca cccaccgtat ctaatcagat tcttggcaac aagataggaa
     1861 ctgatgcaac cggcatgttt ggtattccca ataaagaatc cggtatttac atcaacggct
     1921 cgaatcacaa ttataactat atcggcgatg gtactaccgg tggtcgtaat attatttccg
     1981 gaaacgacgg cgatggtatc tatatctacg gcagtgcctc tgataatttt attttaggca
     2041 actatatcgg cgttgatgca accggtaata ccggattagc caatgattcc aacggtatcg
     2101 cgatttacaa ctccggtcac attctgatcg gtgatgaaac ggccggcggt cgtaatatta
     2161 tctccggtaa tggaaaagaa ggtatcgcta tttacgctca aagcaccgag attgtcattg
     2221 gcgacggagg gaacaaagct ctggtcaatg atgcgggagg ccgtgttgct aaacccgaat
     2281 taccaggggc gaataatata gatatccgtg gcaactacat cggtgtcggt acaaatggat
     2341 caacctcact cggcaatggt ggtgccggta ttgaaatcga agctgtcttc gaaggttttt
     2401 attctcaata cgattcgatc acttcaaatc tcatagctta caacaccaat ggtatcgaat
     2461 tagtaggcgc cggtgtttcg tcgacctaca ttcataacaa tacgatttac ggaaacacgg
     2521 cggacggtat cgcaattgaa gaaggcgctc aaaacggagt caccactccg gcgattacct
     2581 ccatatccga tttcgtcgtt agcggtacgg cttcggttgg cgccatggta tatgtttacc
     2641 aggacgccgg caatcaggga cgctactacc tcggttcttc cgcggctgac ggcgaaggtt
     2701 catggtcata caacggactt tacttacccg gttataatgt aacagcaacg caatttttag
     2761 atgaacaatc ttcggcgttc tctgcaccat tcgctacacc gtccggcact atacatgcta
     2821 cgaccacatc gctttcttac ggcaatgtct tagccggtga tagtgccaca ctaaccacgc
     2881 gcatttatgc cataggtaat cctgtggtaa tgaatagcgg ttcgttagta ttgggaacga
     2941 atgcacagat gttagccacg ttaggaaccg acaccctctt tatgacggat acgatcacgg
     3001 tacaagttcg tttcaaaccg gttaactttg gatttcttac cgatacactc cgtatttatt
     3061 acggtgaagg taatatgttt gatattcgta tcggtggaac cggcacgtcc ggtacaatag
     3121 cggccagcag catcggattt gcgatggaga cactcaatgg cgacagcaca agcgttccta
     3181 tccagatgta cacgacaagc ggttgggtgc aattgaactc cgctacattc ggtcagaata
     3241 attatttcac ttattcgatc gacaaaacgt taccttacat aatgaagccc ggcgatacag
     3301 ccggtgtgac ggttacattt aaaccgacaa catttaccaa ttcggccgac acgctgtact
     3361 ttgcaaacaa ttcatccgat ggtatacttg aattgatgtt tagcggttta ccgcttccgg
     3421 gtacggtagc gacgaatctt gcatcccaag attttggtaa tgttctattg ggcgatagct
     3481 tggaattgac gacaaagatt tacgctccgt caggccgggt cgaaatcaca tccgtcacac
     3541 tttcccaagg tgatgtactt tcgatgacgg taacgccggc attacctttt acgttgaatg
     3601 caggggacac catcctcgtg aaaacgaaat ttattccgtc tgattttaac tcgtttaacg
     3661 acaccattac ggttttaagt aacgcctcca acggaaccgt tactgtgtcg ttgtccggac
     3721 aatctttgcc cggaaccatt tcgtctgccg cttcggcgtt cgattttggt aatgtgttag
     3781 taggtgacag cgctcaaacc tcaacccgtt actacgccac aggcggtccc gttcagataa
     3841 ccggttcttc actggtaaca agcggtgaaa caagtgtcag ctttacaccg tcattgccct
     3901 atttgctgca cgtaggagac tctgttgtcg ttaccgccaa atataagccg ttaacatttg
     3961 cggcgatgag cgctgacacg atccacctga tcagtaacgc atcaaacagc ggcaaactga
     4021 gcatcccgat gagcggtttc ggtcaaaccg gtacgttgac ctttaatact acaccggcta
     4081 atttcggttc gattcgcgtt gccgatagca gcacggccca aatcgtcaaa gtatacagca
     4141 gcgtaggtca gatcccgctt acaggatata ccatcggctc tcatttcaaa gcgacgactt
     4201 cgaccgtatt ccctatcacc atcaaatccg gtgatacggt tacgttagct gttaaattta
     4261 agccgcagac caccggcgct cttttagaca ccatcaaaat cggaaacgta cgtattccgc
     4321 ttgcgggcac cggtttagaa aacgtggcgc ctacgcttac gctcggcgtg acaagcgtca
     4381 tcgcactcaa gcagtacctt ggtatttaca tgtatagcag cgaggcgttg agcgctagat
     4441 cggctgcatt taaatttaat gatgtcagtg tcaccgcacc gacatttgat gtcgtatccg
     4501 gtgtaaataa cctgtactat gcgcagtaca aattatctgc ggccggttcc ctcaagattg
     4561 acgctaccgg tacggacatt gccggaaaca tcggtacgcg ttccaaaacg tacacgatcg
     4621 gctcattgac caaagatcaa actttggctg tcgaagcgga aggtttgcgt atcagcggcg
     4681 ctaaaggcgc tatcggtgaa agcggtttta tgattattac caaatcgcaa acctatgacg
     4741 gcgaccctgt cggtttagcg aaaggtacgt ccaagtcagc gaccgatttt gcgaatgcca
     4801 atatccaatc atgggatatc atcagcaccg tagcgcttga aaaatccgtg acgctgacgg
     4861 ccgtctatga cgaaacatgg atccatgcat tacaaacgca atacgccaac ttcgacgaac
     4921 ggaaaatcgg tttctaccgc gaagagaacg gctcattggt ttatctgggc ggtgaaggtc
     4981 gcaacggtaa cgttgcactt caaaccaaat cgcttggtaa gttcgtgatc cagtacaatg
     5041 acgaacatcg ttacttgcct tcagaaatta cgttggcgca gaattatccc aatccgttta
     5101 accccacgac aacgatcgaa ttcggccttc ccgaagcctc gcacgtcaaa ttagtcgtgt
     5161 ataatatcct cggacaacgc gtcgcagagc ttttcaacgg ccagcgcaac gaaggatatc
     5221 ataaattcgt atggaacgga cgcaatgcac aaggcgccac ggtggccagc ggcgtgtatc
     5281 tgtatcgcct cgaaaccaac aaaggtaacg tgactaagaa aatgatgttt atcaaataac
     5341 gtacgatata taaaaaaatt atataacaaa ggatcatgat tatgaaacac ttcaaagtat
     5401 ggctggccgg cgcactgatg atcgcgctta gcgcaggatg cagcgaggat ccggcgaccg
     5461 accctctcgg ggatgcatgg cagcttttta taaacggtca atacgctgaa gcacaaacca
     5521 gttttgaggc ccttatcgaa acgcaacccg gtcaagccta cgccgggctc gggtggacgg
     5581 ccatgaaatt agacaatatt ccggcatcgg tagattattt cgagcaggcc gcgggtgatt
     5641 cacttgtgca tggctacgca ggttgggcta ttgcgtcatg ggctaacaaa gattttacgc
     5701 atatcgtcga acgtgcaggt tttgtaatac gcaaagaacc ggcattcgaa tttgaattct
     5761 acaaagatat taccatcagc accatgcgtc ttatgcaagc ctacggttat tatcatacag
     5821 gcaatatgtc ggcctgtctt gcccgcattc aagagttgga tggtggtttt gccagtacaa
     5881 cagatccggc attattgctg gcaaaacttg acgctttaag cgcgaccgct tccggattat
     5941 aaagcgaact ttttataaca aaaaaaccgc agcctaaaaa ctgcggtttt ttttatgtgt
     6001 aatttttaca gaagcatctt tttttcatca aaacgtgccc gtaaaaccaa agtggatttt
     6061 cccgtccgac ggacgatcgt tttgaccgag accatagtcc agagcaaact gcccaagccg
     6121 cgtcccaaat cgcataccaa agccataacc ccaggggaaa ccgtcttgtt tgttaatttt
     6181 cgtacgttct aaatcagcat atgctctgcg gtaataataa ccttgatcgt aaaatacaaa
     6241 caatcgtgat gcgcgccctg taacataacg caattccaaa ttattccaac ctacgcgcgt
     6301 tccgctaaaa aagccttcgg tataaccgcg cagagttttt aggccgccaa ataaaaactg
     6361 ttcactcagc ggcgccacat cacgaccggg tctgaacact gaaaaatgta acccgtttgc
     6421 aataacccag cgatgtaccg gttcacggta aatttccaaa tccatacgcc atgtttgggt
     6481 gcggatggat tgggaaaacg agcgaatgat tttttgctcg ccgccatagg tgatcgtatc
     6541 cgtcccgtcg gatgacagag tccaattttt ttcccgtgta cgtccatacg aaaaaaccgt
     6601 atgataatat aaaccattgc ggggattaat ttgttcgtca cgcatatccc cggtcaagcc
     6661 gccattccac gcgtaaccat cgctctgcgg tgtattaaat ataaacgcag ttttcggatc
     6721 ggcgggttcc accgactgta aacgcacccc cgctaaaacc gaccaatcgc gggtaatttc
     6781 ggtttgcaat tccatctccc aggcgctgcg cgaatacaac gaatcttcga tttgctgttg
     6841 cacggctatg cgtccgttta caggccatcc gactaaccac ggctcggtgt actgaaccat
     6901 cagatcctgt gaaaggcgat ttttcttttg ccagtgcata ctcaactgcc gcgccgttcc
     6961 aaaaagatta taaaacgata catgaatcaa cccggtgaag tatccttttt catcacgcgc
     7021 cgcatcgcgc gggacataac cgataatccc gtccattgta ttagcacgat cttcctcgac
     7081 atccagccat accgtcaaca gggaatccgg tctcattgta accgaagtaa gttccgcact
     7141 gcgaataaaa ggaaatcgtc ggatacgcgt aagaccgtct tgaatggcac gatgatcata
     7201 accgcggggt aatttcatgc gcatttcacg cgtcaaagtc agcggtttgg tgctttcgtt
     7261 tccccgaacg ataaattcgc cgaccggttt gacgatacgt cttttgatat gcaacagaat
     7321 atcaattata aaatcagact tcgcatcgcg tcgcatccag accgagtcaa gcgctatatg
     7381 tacaaacggc gcaccgttgt tttcaaacca gtccagcatg cgatttaccg atgccgtcca
     7441 ctggcccggc gagaacatat cacctttttt ccatcgcata acttccgtaa tctgcgacgc
     7501 tgaaatatcc gattcatcgc ttatgcgcac agagccaaat ttagcttgtt tttcttccga
     7561 cagaaaaaaa atcaaccgta tgccatctgt gccttcagct gatttttgtt gaatggaatc
     7621 aatggacgac gccaaataac cccgatcacg atagtattct aaaatacgca tcgcatcatt
     7681 gcgcgattgt tcgtgatcaa aaacggaacc gggtttgtga tagagcagag ataagagtgt
     7741 tttgtcattc acgatacgat tgccgtgaat ggaaacagaa gcaatacgct ctgttgcggt
     7801 ttgcgcaaaa agcgtagttg tgaaaaaaaa tataaaccag cgcataatta ttaacgctct
     7861 cataattgag cttatatgcg tattaacgaa caccaattct ctcacgcaga gtcgcaaagt
     7921 aaaaatgttg actgtgcatt gtctcgcctc attggttccc gctttccctt cgacttcgtt
     7981 cagggcatgc gcgggagcga aagagcagcg atagcgttgt ctcacccgcg cacgcgggtg
     8041 tccatatatt ggcgggtagt tttgtgttgc tacgtccagc ttcctctcat tgggttccgc
     8101 ttcccattgc ctggtgttcg atcacacata gacgtaactc acgccgagat ctcgggacat
     8161 atcgcgggaa caacagcgcc gttatttgcg tcattgcaag catgaaatct ttctcgcaga
     8221 gccgccaagc attgatgcat tgattatgcc ttgtcaaata tgaacgcctt aatatcacaa
     8281 tgggatcacc gtagcgcggc cacgggtctg atgggttatc gcatcgcaga agtgcaaaac
     8341 ttcatttttg cgaatcgcta catccatcgt ggcttgctcc gaatatgcag tttcccctgc
     8401 tttcccgccg gcatgatgta cacagcgcat gatcgtattg atttcatcgt atggaaactg
     8461 tatccgaagg ttttcgtaga tcagcctctg ttctgcacaa catcgcgaaa gaacatccgc
     8521 ggccgaatca ccgtacgcac gtacaagtcc gcccgtgcca agcttggtac ctccaaaata
     8581 acgaacgaca atgacgataa tatccgtcca gtttttagcc gtaatggcct gcagaatcgg
     8641 tttacccgcc gtaccggaag gctcgccgtc atcgctgaga cgaactgaaa catgaggtgt
     8701 taaacccaat tgataagcga agcagtgatg cgtggcatca aaataatttt tacgaataga
     8761 ttgcaaaaac tgttcggcgc cgtctttatc ttgaaccggc gcggcatacc cgataaattt
     8821 cgaaccttta actttgattt cggacgtaca tgattcggcg atcgtgtagt attcatccgg
     8881 cgtcatgcgt ttttccgagg gaatcacgat gcgtagaaaa tttttccgtt atgtataacg
     8941 ccgacggctt tgcctttcat cgcataatcg tgatacggtg tgttgattga tttcgaacac
     9001 gtctgatgca catcgtattt ccagcgcatt tccgtatcga gaatggtcag attggcttgc
     9061 gcaccgatga taaactccgg cagcggaaga tgcagaagtt tacggggtgc gatagacaat
     9121 ttgtacaagg ccgactccag cgaaatcacg cttttatgta cgagatcatt gagcgtaata
     9181 ccgacgcatg tttccagtcc ggtgatgcca aacgacgctt cgtccatcgt acattcttta
     9241 tcttcgatcg tatgcggtgc gtgatcggtg gcgataatat caatcgtacc gtcttgcaaa
     9301 cccaacttga tttcttcgat atctgcagat gtgcgcagcg gcggattcat cttagcgaag
     9361 gtattgtaat tttccacagc ttcgtctgtc aacgtaaagt gatgcggaca cacttcacaa
     9421 gtcactttga ggccgcgttg tttcgcttgg cgtaccagac gcacggttcc gcccgttgaa
     9481 atgtgtgcga tgtgaaccgg agcatccgta tattccgaga gtaagatgtc ccgtgccaca
     9541 ataacttctt cggaaatgct cggaatccct ctcagcccta atcgtgtgct ggtgaatcct
     9601 tcattcatcg aaccgcgatt agaaagttgt ttatcctctg catgctcgat cacagggata
     9661 ttaaacatct tcacatactc cagcgcacga cgcatgattt ccgcattata caccgggtca
     9721 ccgtcatccg taattccgac cgcgccggct tcgaccagac tcccgatttc tgctaattct
     9781 ttaccttcac gtcccttggt gacgcaaccc acgggatgca catccaccaa atggccttgc
     9841 gctttatgtt ttacgtactg cacggtatcg gcggtatcta tcggcggcgt tgtattgggc
     9901 atacaagcaa tagccgtaaa gccgcccacc atcgctgcgt tagctccggt ccaaacggtt
     9961 tctttatctt cgcggccggg ttcacgcaaa tgtacatgca tatccataag accgtgcgta
    10021 acgaccttgc cggagagatc aataactcta tccgcagggt catcgcattt accgatgcgc
    10081 acatagcggc catcggcgat aagtatatcc acgacttgat ccgatttatg tatcagatcc
    10141 aaaagtcgaa catttttgag tagcagtgtt tgcggtagtt tttctgcttt gacgtcgcgc
    10201 gccatgttag tgtttttcct gtgaaaattc gtaaataata taaatcatgg tgactaaaat
    10261 cagactgaat acaagggctt tcatctcacc gccttgtaca tcatatttta tcccggtgat
    10321 gtccgataat tcgaaatcac gaaacgaatc gtagatgcgc atggaaacga tgccgtccat
    10381 gtacgaataa aacacgccgc ggtgaatatc gccgttagtc gtacgaattt ggatcacgta
    10441 accgggtttt attttgtaca taaacgccga ttctatttct ttgcggcgtt tttccttttc
    10501 cgtttcatct ttcggttcat cggaccgcat atagtaatta tgcgaacatc cgacgaacat
    10561 catccatccg aacagcatca cccacagtaa gcgttgcgac atagagactc cgtttcttat
    10621 taatgcggtt ggttacgcat ggcatcagcg accatgtaaa aaaactcatt gagcaaatca
    10681 atcatccccg gctcatgcaa ctgttcgcgc atagcttgcc ccatacaagc catccactgg
    10741 tcgcgggcgt ttccattgat cggaaacggc gcatgacgca tgcgcaatgc cggactgcct
    10801 cttttttcca tatagaccgg cggcccaccg aaacggccca ccaaaaacat aaacaattta
    10861 tcccgcgatt cggtaagatc ggtcggatgc atcgcacgta tttcacgtgc tgaggaatca
    10921 gtatccatga tatcataaaa acgatcaact agtttcctca aaccttcttc accgataacg
    10981 gaatacaacg taccaataac ggctaactta tcctgatcca taccgcttta ttttttctta
    11041 cctttgcctt tagttttttc ttttaccgga tcacgcggca agtctttttc ttgcggttta
    11101 ttttgttttt gttctaccgc aggcgacatc gactgaccta aaaaattaac attttcgacg
    11161 acaaacggaa gctcgacgcc ttcgacatca tcatggatat gaatattgat cgttccacgt
    11221 ttgggcacgg cggtattcag attcaacacc atcgtcgtag attcttcagt atacgtactg
    11281 atataccgat ccgatatacc aaccggatta cctttttcgt caaggatatc cattttattc
    11341 aacttgaaat ccggcaaacc tacacgaatc accatctgat gcgtatattc atcatcctga
    11401 atatcttcaa tcaccgcacc gtattgattg ccttcggcgc ctttttcaaa ctttcgaata
    11461 ccaggatcca caacacttgt ggacgtgctg cgcatagcat acaccgttcc ctcaatgcgt
    11521 ttgatgcctt tggccgtagc atccggtacg gtcaatgtca gcgtccaacg taaaaaatct
    11581 ttttgtttgg acatttcgac gtagttggtt tcgcggtacc attcgctttc atgcaaaaga
    11641 ttttgtccgt tgtcggcgat agccgcggtg actacggcgc gttccgtttt gacgattgcg
    11701 cccggcaacg cacccgtaaa tgttacttga taactttgat ccgtaccaaa ctggagataa
    11761 tccggatctt cgacattgga ataggtaacg cgggtgattt tgacccaatc aaaagttccg
    11821 ccttgataac tctgcacggc acgttcgggc tttgaagact ccatcgtcgc cgaagattcg
    11881 gtttgattga ggccgagacg ttcgatcatc gcatcataac cggctttggc cgcagaaact
    11941 tcggcttcgt aattaaattg aggcgctgtt ttaccgctca ccacggcacg caccgggcct
    12001 ttttgaccga aaagtaattc gttgaccgca tcgccggacg gcggattgtc tttgatattg
    12061 gcgcccgcac gcgcttgctc tttaagccaa ttttcgtcgg caaataattt atctaatacc
    12121 tgaatgaact tctcaccggt aaaggaaacc tgatacgaac cgtcggcctg ttgggtcata
    12181 ttattagacg actgcgcttt gccgggcaaa cgaaaattca tttcgctttt catcgtaccc
    12241 ataatcgcca acagcatcgg acgcatttgg ttataaccgt ttttttgttc ttttacggct
    12301 ttggttattt ccgcatcggt ggtcggtatt ttttctgaac cggatttggc tttatcggat
    12361 ttttttgagg ggttcgttgc caattccagt acccagttct tttgtgcatc ctgatatacg
    12421 acgatgctgt cgccagctcc ggcattctta aattttacat tggaaatatt tttgaaatac
    12481 gccgtgcctt tgaaataatt gcgtccgtcg tcaagtacac gaaattcaac atctttccat
    12541 gtgtctacac ccgtcgcttg ctgaatgatg tcgcgcgctg ttttggaacc atccggtgtt
    12601 ttaccgctca tattgcccat catgccatcc ataccggcgg tcgtcacttc gaccgtcact
    12661 tttcctgaac cgtcgggatt gatcgtataa tcggctttgg tttcaaagca accactccat
    12721 acgaccgtag ccaaaatcat caacaataaa ggcatcatgc gcttcatata ttttctcctg
    12781 ttatatattt tgaatgtaaa acaaatacgc tcttacatca attggaaata cactgttttg
    12841 agatacaatg tttccggtat ctgaagcatc cacggatgat cgggtggctg ataggtgata
    12901 ttgactacac gcatcgaaac accattgtcc gcggaagcaa accgtgcggc ctctagcatc
    12961 aggtccaccg gcatgtgata cgcgcaagat gaaagtacga tatgcccgcc cggattgaga
    13021 agttggatcg cttggtgaag caacttccaa taagcccact tcagtttttc gatgccttct
    13081 ttgcgtttag cgatggccgg cggatcaata atgatcacat cgtatttttt caccttgtcc
    13141 ggcgtcgaag ttttgactgc aacttgctct tccatatact tgaatacatc ggcgtggata
    13201 aattgcaatc gcttctcgac attgttacga cgggcattgg cccggccacc ctccagagcg
    13261 gcggcatctt gatccacggc ggtcatacgc acaccggatt gggctaaagc tacagcgaat
    13321 ccgccggtgt aggaaaaaag atccagtccg gtttgattag gtttgactaa tgtacgtaca
    13381 tacgcgcggt tttcacgttg atccaaataa aaaccggttt tatgcccatg atggatatca
    13441 acatgaaaca acaaatcatt ttccgatatg acgatttctt ccggtatcgc accatacaca
    13501 acacccgaaa aaggctccaa cccttcttct tcacgcgatt ccatgtcgct gcgatcatag
    13561 attcctttgg gtttaacgac ttcgaccaaa gtttctaata tttcagtacg aaatttttcc
    13621 atccccagcg agcgaaattg cactacaaga tacgcaccga attgatccac catcaaaccg
    13681 ggcaagcgat cgccttcggc atgaatcaac cgccgtgcgt ccgatggcag cgccaattgt
    13741 gtacgaaaat caaaggcacg ttgaatacgc tgcttaaaga agtttttgtc tatcggacga
    13801 ttttttcgat ccagcacccg cacgacaata tgagataacg cgttataata acccacagca
    13861 agaaacttat cctccgcatc ggcgatttcg acgatagcgc cattggggat atcaccgcga
    13921 tctttgaatt gcgtggcgat atcatcacga aatatccacg ggtacgcatt gcgaattttc
    13981 ttttctttgc cggattttag acgaatacga tgcatgaagc agtccaagaa atagcttaca
    14041 taaatttgca ccgcaatcta catgaggaaa cgcagaatat caaaagattt aaggtaaaca
    14101 acaggtcaat ttaaaaaaaa tttagaatat gaattagaga ttgacgctca ccaaaaacct
    14161 gcatgtttat gtaggttaat ttcaaatcat tataaatcat attgttatgt aaacattaaa
    14221 caaattttga cgcttaaatc gtcatcaagt gtgcgttaga tcacatacct cataaaaaaa
    14281 atagtctatt atcattggtt atgataatag actatgaagc gtattagtaa aaaaaacatt
    14341 gctcggccgt cattttatag tagctttatg gtagctttat ggtaaatatt tttcgtagct
    14401 ctttttgcca tgaacataca ttatttattt aataggtttt aaaatcatac atttcattaa
    14461 accaaaggat tatttctatg cgtaaactta tgacgatgat tgccttatta gcgctttgta
    14521 gcgtacagac ttacgccaaa acatggaccg tggatcagag accaggcgcc aattttcaga
    14581 ctattgccgc ggccgttgct tcatcttccg tacttgcagg ggacacgatc cttgtatctg
    14641 gttcatccat tgcgtatgcc ggatttagct ttaacaaaaa actcacgatc atcggcccgg
    14701 gatatttcct tggagaaaac accaatctgc aagctgacac caattcggta aaaatcagtt
    14761 ctcaggttac gattcaaagc ggcgcgcaag gatcaattgt catgggcttg catttcttaa
    14821 acagcctata cgttgatgcg gacaatgtac aaattatacg taaccgcttt caaattacga
    14881 gtacatacga ctacggcatc tatttatatg cacaagacag taccattatt cgtcagaatt
    14941 atcttacaca aacagcatcg tggtatcact gcatcactat tgcttccggc aacactcaga
    15001 ctatgattca aaacaatttt cttgagtaca cgggtggaga ttctaattca agttatggcg
    15061 cgctcagtgt gcaagggacg atatccggtg acatttccaa taacgtcatt tatggaaatg
    15121 tgactttgtc cgcgggtggg ttttatttta ataacaatat tttgcgttat gggaaattca
    15181 attcaaccgg aataacaccg cataataata tcggaagcag ttcgcagttt tctttctggc
    15241 ctgctcttgg aaatcaaagc ggagttaata tgacgtcggt tttcgtatct tccggcacat
    15301 ccgacacgaa gtggcaaatc gctacggaag gcccggccga taataatggt tccggaggtg
    15361 ttgattgcgg tatgtatgac aatagcgccg gcacagctta tatcccgtcg ggtattccgc
    15421 cgattccgtc tatttatcaa tttgatgcat cggatctcaa taatgtcacc gtcaaagtga
    15481 aatcacataa ctaattctat ctgcaaaaaa tgcgcatcga attagttcaa ccttcatttg
    15541 aacgggagtt aacatgaaat tttttacagc ccttatcggg ctatggctca gtgccacatc
    15601 tttattcggg caaagcccgc cggatgtgca ctctgtcgag tacttcatag ataccgatcc
    15661 cggatttggc ttaggcactt ctgtagcacc gagtgcagct acttcgggtt cggataagac
    15721 gtttgtattt tccgtttcca caaccggttt accatcgggt ttccatacac tgtatgttcg
    15781 ttcacgggat aacaacgggg gttggtctca agcaacagca aaagcctttt atgttatgcc
    15841 cggcgaatcg ggttcgtttg caactgcggt gcagcgcgta gagtatttta ttgatacgga
    15901 tcccggcttt ggcaacggaa tagcgatggg atccccctcg tcgggagatg cgacggtgac
    15961 aggcatgatt gacatttctg cactccccgg cggttttcat acattgtacg tacgggcacg
    16021 cgacggattt cataagtggt ccgtactcaa tagtcgtact ttttataaat cggtagtata
    16081 tacccccccg acggatccgc aagtcacata catcgaatac ttcgtagata aggatccggg
    16141 atatggtaac ggaacatccg ttccgggatt tacggcttcg tcggatatca cacacaattt
    16201 tttagtggat atcggtgcgc tttcaggtgg atttcataca ttgtatattc gcaccaaaga
    16261 tgccaatggt aattggtcta tcgcatctac gaaagccttt tataaaccac acaactatgt
    16321 tgctccgtct gatcctcaga tcacggcgat ggaatatttc atcgacaccg atccgggttt
    16381 cggcgcgggt acggccttga gctttaccgc ttcatcggat gtgacggtca acggcgtggt
    16441 agatattact tccgtcagcg ggggttttca tacgctctat ttgcgcagca aggatgccaa
    16501 tggaaactgg tctgtcgcgc aggcgcatgc gttttacaaa gcgcataccg tctccaatac
    16561 atcgtctaac atcacagcca tcgagtataa tttttacaaa tccggatcat cgacaccggt
    16621 acggacattt acggctttta ctccttcaac ggatgttcag acgacgtttc tggctgatct
    16681 atcaggtctt aacatcgaca gcacctacga tatgcgcgtt acggcgattg acgcggcagg
    16741 acgtcgcagc attgcggcta cggcatccac cgtcatcatc gaacgtccta atcatgcgcc
    16801 ggaaattaaa tcatcggttg ccgatgtgac cgccaatgaa gactttgata gcctgatcgt
    16861 cgcgtcgctc gacacggtgt ttcatgattc ggatatgacc accgtaggcg atcatttgca
    16921 attcaacgcc tcggtcaacg gctcagccgt gacttctgtg ttgcgtggtt cgcagctggt
    16981 aattttttct tctccggata tattcggatc cgccgaagtg atcatcacgg ctaccgatga
    17041 cagcagcgca tcggtcagcg acacgataca tgttaccgta aattccgtta acgaccggcc
    17101 attggcaacc tacgatgtcg cgcaagccaa tgcaggatat acggcgtatg tccaaccgct
    17161 caaaaacgat acggatattg acggggacgc cctcgtcatc gcagcgatcg tcacgatgcc
    17221 gcaacacgga acagctacgg tagtaagccc ggcggatacg ttgatttcct atacgccgac
    17281 agtaagttat agcggtatag actcgatcca atatgccgta agcgatgggc tcggaggcct
    17341 tgatacagcg atcatttatc ttcagatcaa cggttatatc aattcagccc ctcagcgaaa
    17401 caacaccatt gccgatgtta ccgcgtcgga agatttcggc gttatcgcat tagcacccct
    17461 caattcggta ttcggcgata acgatatggc gctcaacaac gatagtttac ggtattccgt
    17521 ttccgtacaa ccggccatcg ttgcattaag tttgcggcac gatacggtgg ttgtcaattc
    17581 gatcgctaat ctattcggca tcgcggaagt tatcgttacg gcgaccgatg acagcggcgc
    17641 tacggccagc gatacattga ctgttaccgt taattccgtc aacgatgcac cgttcacaca
    17701 aaacgacgcg gtttcaacga cgggtattca aacggtcgta gcgcatcctt tgaataatga
    17761 caccgacgtc gagagccaaa cgcttacgat cgcggccatc ataacgtcac cccagcatgg
    17821 ttcggcgacg atcccgtccg gtgatacgac gatcgcttat accgcaacca tcggttacag
    17881 cggcaacgat tcgctgacgt attcggtcag cgatgggaac ggcggtttgg atacagctaa
    17941 gctcgtcatt acgatacaag ctccggcgcc gttgagtctg agtacatcgg tccaccaaaa
    18001 tccggctttg gcacaatacg ccgacgtcta tgtcatcgcg gatacggcgc tcatcgggtt
    18061 gcccgacgtc aaaatgtttt tgggaagcga ttcgaccgcg ctgacgatga ctgtatctac
    18121 atcgcgggtg tatcgcggac aatttgaatt tgcaagcagc ggcgcttata cgattcgtac
    18181 acgcgctacg gccgccaccg gcgcgcaggc tgtgaacttc cgttccttta cggcgacttt
    18241 ggccaaaccc ggcgtgacga caaacatcgc ttcgaccgac gcccaagccc agcttttggt
    18301 gaaatcgaaa gccattgttt caccgacgta catcaccgtt gcgacggatg aatctatgaa
    18361 cggcaaggcg tatcaattcg cacccgcatt gacactgcag gaaccgtcgg aaataacgat
    18421 acggtttgaa ccttcgcagt acgaagatgc cggtaaaatt tttatcgaaa gcgaaacgga
    18481 cggtcgtatc gtacgtctgc aaaccggggt ctatccggag caacgtatag ccaaagccaa
    18541 gatcacttcg ctcggtaaag tgcgcatcgt gtatgatccg tcgttcaccg gttccaatac
    18601 cgttccgaca aatttttctc tgaaagccaa ttatccgaat ccgtttaatc cgtccaccac
    18661 gatcgcttac gatctggcga aagaaggtct ggtgacattg acggtgtaca acgtactcgg
    18721 acaaaaggtc aaaacactgg tgcatggtca tgcacctgca ggttcgtaca aattgctgtg
    18781 ggatgccacc gatgaacgcg gcaaacgcgt ttctaccggc gtgtattttt atcgtttaga
    18841 aacgcccggc tttgtacaaa cccgtaaaat gcttttgatg aagtaggagt aatcatgaaa
    18901 aataaaatta tcttttgcgc actggcgatc gtgttgttat cgagttgcgg tcccgatccg
    18961 ggaacatctg aagattatgc ggccacggta gccgaaggct ggacattttt tgaaaccggt
    19021 tcctatccgc aagcgaaaga acgttttcgc gcggccatcg cgatagaccc tgctttttca
    19081 gaagccttta ccggtttggc atggtcacag atgaaaacgg acagcttaga tgaagccgtc
    19141 actgttttta gctttggttc ggctacgtcc gacgccaccg ccgatttatg ggcgggatgg
    19201 gcgtttacgg aacatgcgct gaagcagtac accgcttcca atggtcgcat cacgaatgcc
    19261 atagccgcgg atgcgaactg gatatttagt cataccgcag gattatcggt cagcgatctt
    19321 cgcgttttga cggcagaaaa tcactttgca ctcggcagtt atgcggccag tctttcggcc
    19381 gttcagcaac tcaatgcggc ttttaccgcg gatgtgacga ccaccgccgg tcagtctgcg
    19441 ctcgccgctg agattgagcg attgaaggga ataaactaag tctttatctc aagggtcata
    19501 aaaaaaggcg aagtgcaaca cttcgccttt tttattttca ggatgtgatt tgcgttcggc
    19561 gggattttta gcactgaact ttcatcgacg aacagcagct gaattttgcg cttcctttga
    19621 tacgaagccg ttcagcccgc ctattgccaa tacgatgtca gcggctggca atttttccaa
    19681 gttttaaaat tagtttccaa gttatttctt tttttgtttc aatgtctttc caatgttctt
    19741 gcaacttttg cgacaagtgc tctataggat tttcattata tttgtcaata tatttttgag
    19801 ttgcagacca acttcttaaa tgtcctaatg tttccgaaag tgttactagc cttttaatga
    19861 aaaaatccgg tgtttttatt tcgtcaaaag gaaatggtat tgttaaatat tctttttcaa
    19921 tcagtctgtt ttcgggaagc caaaattcgc cgacaatatt gtcgtgaaag tttttaatga
    19981 tcttgtctaa ttcttcgttt attgtcggaa taccataagc ccaaacggca attattccat
    20041 ttgtctttaa aactttcttt acttgtgtgt aaaatttgtc gaagtcaaac caatggattg
    20101 cttgggcaac tgtgattaag tccacagaat tgtctgctat tgcacctgga ttttcggcat
    20161 tttcaacttt atagactatt ctgtcgtgag gaatagcatt ttttatttgt tcttggcttg
    20221 ggtcagatgc ataaactttt tcgtagtaat cagtcaattt tatagctgat tgcccgtttc
    20281 ctgttccaca gtcccacgcc aatttatgtt ccgcagtcaa accttgtaaa aactcaaaca
    20341 attcttttgg atatgtcggt ctaaatttgg cataaacttc tgcctgcttt gaaaaattgt
    20401 ctttaaatgt gttcaatttt ggttgtcgtt ttttacttgc cgctaacggt ttgcagcttg
    20461 gcgaagcggc ggatatttgg tactaaagtt caatagaatc gctactgttg agccttgcaa
    20521 aaaagtttca tggatgcact gcacccgcca ttttgcaaaa ccaatgttag tggctggcat
    20581 tattacgctc taatattttg tcaaagtaca ataatgtttt ttctttgtca tttttttcaa
    20641 acatatctgt aattaaatat tgtaaactgt ctggtgttgc ttttttcaaa taagtaagca
    20701 tttctataga tgttgtatag ggtggcggtt ggaatttaac tttatccaaa tattctttta
    20761 acgcagtatt taatttttca actccaataa attcgctaag tgtatagagg gcaatagctc
    20821 ctttcccata agcaatgtag gactgttcag gatttactaa gtataagggc ggttcttgtt
    20881 ctgtttcatt tgccctgccc aataaatatc gtttacgctg tatctgcaaa aatttcaacg
    20941 cactttgtat gccatatttc ttttcataaa tttttgcggt aatgtattca gctatacttt
    21001 ctgttatcat agttgcacct aaagcattag caggtattac ttgattgccc caccattgat
    21061 gcgaaagttc gtgtgcagca acataaaagg aaaggtcaat tccatcttct tttaaattac
    21121 gagcatcatt gataaaacgg atttcagaaa tggggacaca gtttcctgct gtagtcgcat
    21181 aagaaccttc agaccttgaa aattcaataa tatgaacttg cttgtattgg taagtaccaa
    21241 aatgcaacac attataatct aacgaagctt ttaatccgtc catcatttgc tgaatgttgt
    21301 atatgtgttt tgggtggtaa taaacccgta aatcaactcc tttatagttt tcttttaata
    21361 ccgcaaattc gcctgagtta aagccgaaaa caaacttgaa gggtttgtcc attttgtaat
    21421 gaaagtatct tttgccatct tctaaccatt ccctcaataa atttcccgat gtaatcgcaa
    21481 tttgttgagg gcttgtacta acaatagcct caaaatttac taaatcggca tcaatgcttt
    21541 ggtaatgatt ggcaaaagag ctactgttgg tgggtatctt tttttctgta tcagcgaaat
    21601 atcccaatcg tggaaatata tcggatttta aataagttcc attttttaaa atgttggaat
    21661 tttgagttaa aagcgtattc ttttggtttt tgattgtaaa gtttaagtta atgctgtcat
    21721 tgggtgaaac tcctttatca agcttataca tacaaaactc aaaaatagtg tcttcttcaa
    21781 ttaaagcagc ttgttgatca aaggaaagtg tggttatttc atcgaatcct ttttttatta
    21841 aaagcgtgtc aatg
//
LOCUS       DAOQGF010000051        21641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1111_length_21641_cov_6.848745, whole genome shotgun sequence.
ACCESSION   DAOQGF010000051 DAOQGF010000000
VERSION     DAOQGF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_21641_cov_6.848745"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..700
                     /locus_tag="PK937_08580"
     CDS             35..700
                     /locus_tag="PK937_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNH32665.1"
                     /translation="MEQLIYLPVLILAVVVHECAHGLVAKWCGDDTAYREGRITLNPI
                     SHVDLWGTIIVPLVLALSSAGLMFGWAKPVPVNPNNFRHFRRDDILVSMAGPASNFII
                     AFISALFIVLLSLVWKAASPESASSMQFGEFALELFKHSIQINLYLALLNLIPIPPLD
                     GSHVIANLLPPHLGEQYRSLGFIGIFILIVMISIGVINAAIDPFIEYLIGLLNAFIVM
                     LAS"
     gene            706..1737
                     /locus_tag="PK937_08585"
     CDS             706..1737
                     /locus_tag="PK937_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNH32666.1"
                     /translation="MQLQYESRTLHLRHPFIISGSQYSEKVVFTATLMHEGIVGRGES
                     SPSRYYGEPPESVQTMLSDTARWIGDISSPADIQTLHETLLQKYPHQPSARSCVIMAA
                     YDWLARSCQMPLYRLWGLNPHKACRTSFTIGMDEPDVLTEKVKEAEAYPVLKIKLGNG
                     DEDYTIMQTIRRCTDKTIRVDANEGWQRDEAIEKITWLATQNVEYIEQPLPKESLEDM
                     VWLKARSPLPLIADENVMTSRDIPGLHHAFHGINLKLDKCGGMWEALRMIQTARALDM
                     KIMIGCMVSTSLAMTAAAHLTPLTDEADLDGHLLIRDDPFRGVTINHHGQLILPESDG
                     IGVTEQGIH"
     gene            2002..3105
                     /locus_tag="PK937_08590"
     CDS             2002..3105
                     /locus_tag="PK937_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH32667.1"
                     /translation="MIQIGEILKQVPFFRSLGRDSIDFITEKLKFKQYKNGTPICKIG
                     DPGDKMWIVLSGKVAVSAANGEVLAQIGQGNYFGEMALLTGEPRSATVSAADDTETFI
                     LDKDDFDVILEKYPSISIAMSKIMSQRLRETNAVVSEKAKELKSQAPAGPSGSLRDKH
                     ITEVLAFCEANSLTGALEVSNAGQTAVIEFEKGVLQTIKFGTMGEDEAMDTLMGWEDG
                     TFVVKPRLIALDGLGSGSSTETPKHESKKTGSILVINHSMVVRKVLEKNLSSLGHTVK
                     TADGITDGIGALRTVKPNVIISDFKYDEISGYDFCMQIRETANYLDIPFIFITDSSTD
                     SAVVNQLKSVSNSAVVDAQDVSHIANTIEKMLP"
     gene            complement(3284..4015)
                     /locus_tag="PK937_08595"
     CDS             complement(3284..4015)
                     /locus_tag="PK937_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH32668.1"
                     /translation="MRSLLMIAFLSVASYAQSQNWQWPDSSKNLQVLPSNTRGTELRK
                     TMSEFINGLGVRCVYCHVGEDNKSLSTFDFVSDAKETKNTARKMMTMVKVINETYIAH
                     ANENKSKPLDVTCVTCHRGAPLPERIEDILASTYATSGIDSVKNQYRSLRSTYYGSFT
                     YDFREGVLLRTASRIKKATDPSDAIALVELNNEHFPNSVRTLITLATLHQEAKHIDVA
                     KSYAKKVLEIEPGNEAAKKLIESLK"
     gene            complement(4084..5379)
                     /locus_tag="PK937_08600"
     CDS             complement(4084..5379)
                     /locus_tag="PK937_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HNH32669.1"
                     /translation="MQALTLIIYYKGVNTYALNTLIGAVQQGIEDPRLHIALAFRRDE
                     LYSKALSAYNQGHRVVVSWSFYSTHSTGVQEDLHWFREQPHTSDITHLAGGVHASSEP
                     HATLRMGFDWVAMGEGEMIIVELLRRLLNGDDVYTTHGLAYIHGETWKRNPPPPLIQL
                     DDFPPFADRWVRVNPIEITRGCIYACSFCQTPFMMKARFRHRSVASVMRYVQIMKENG
                     AWDIRFITPTSLSYGSDDASVKLEAIEALLKGVRQVLGDRGRIYFGTFPSEVRPEHVT
                     PDILRVLKKYVHNDNLLMGAQSGSQNMLDRMHRGHDVSCIEQAVRYSVEAGFLPNVDF
                     IFGFPDETEEDITASLQLAERLTDMGARIHGHTFMPLPGTPLRSSSPGHISERIIEKL
                     HKFISAGKMYGQWIAQRNMASELSKKNSRKEPARATCRS"
     gene            5548..8847
                     /locus_tag="PK937_08605"
     CDS             5548..8847
                     /locus_tag="PK937_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32670.1"
                     /translation="MLKPTAHVLALDPQRTLSQYVQSSWTTETGLPQNTIHAIAQSHE
                     GYIFFGTYEGLVRFDGVHFTVFDRRNTPQLLNNGILSLLYTRDSTLWIGTNGGGLTRL
                     HRGLFTTIDINSGLPHRIVTAIAEGEDGTLWIGTAGGGLAAYYKGEIKVISTDQGLTQ
                     LTVNALCVTKSGMLWIGTNGGLFSIPGSRFSDISGYTVTRHSDPNGSSLTGIQAITES
                     PSGYMLIGTNGSGVYTYRHGAWSSITLRGALTVGAVYAIRSDPDGSVWIGTSGSGLKR
                     IAFIQINSEGLPQSYTVDEAFTNQSVWSLFVDRDGHIWIGTNGGLHRLMNGPFTTYTT
                     QDGLPDDYIRAVTMDRDNDLWFGSRGGLTRMQWVQGKPVWRTFTPKDGLSNISILSLF
                     SEPDQLWVGTYGGGLNRRSGEQTQVYTESHGLGSNVIRAIARDVYGNLWVGTSGGISM
                     ALAPDLKHAQPHNMLFRKFSSRDGLSNDHVYTILPDADGSVWVGTISGLNRISEGRIQ
                     TWHSGAFSSNILAMFLEGDSVLWVGTDGGLVRVNKSWLLKNTKIPTQNKTSELTTLTV
                     NEGLHSDKIFTILFKSPCDLWMSSNRGVFMICKDQVTAFIRGEIPAVHSSYYGIEDGL
                     KTIQCNGASQSAGIITSDGTMWFATAGGLSSIKTESLTENAQVLLPIAEYIIADQKQY
                     WIPADASSIDLPPGTQHVELHYTALDFAEASKIRFQYRLDGLDENWHLAGSRRVAYYT
                     NVPPGSYVFEVKAASAMGNWSSKPTAIRIHVQAFFYQTLWFYALCAVMAAVCAWGLYH
                     WRVRVLTTRQRELQKIITQRTTELKESQAQLVHSEKMAALGRMVGGIAHEVNNPLTFI
                     YGNLHFLHAHQATFRQLLKLIEQGGTAEQNQNMLKILSDAGYASLDEALNETITLIDQ
                     FKHGADRIRNIVINLKHFASVESSEDESVDVHEGLEATIKLLQAQNQLPIEYYKDFTD
                     IPKVHGLSGEINQVFMNVLMNAVQAIEGQWRKGVTRSGHIRIRTESCQLYAKEAIRIR
                     IRDDGEGISEEHRSKIFDPFFTTRTVGKGMGLGLTVCYGIIERHKGRIYLNPDQREET
                     EIIIELPVKTE"
     gene            complement(9002..9376)
                     /locus_tag="PK937_08610"
     CDS             complement(9002..9376)
                     /locus_tag="PK937_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNH32671.1"
                     /translation="MKWIVLVAMIFSVNSFAQENKPKYEMREYFFVMLKKGPNRSQDS
                     VTAMKLQEGHMGNITRMADEGKLAIAGPFGDNGDWRGILILTVKTMEEAKTEVEKDPA
                     VQAGRLIYEIHPWWSARGSKLP"
     gene            complement(9410..10990)
                     /locus_tag="PK937_08615"
     CDS             complement(9410..10990)
                     /locus_tag="PK937_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNH32672.1"
                     /translation="MCHDVDFVVIGSGFGGSVSALRLAEKGYNVLVIEKGKHLRASDF
                     PKTNWNVRRWMWIPLLRCFGLFKLTFFRHVTVLSGVGVGGGSLVYANTLPVPKENFFT
                     SPSWSHLADWKEELKPFYPLALRMLGATRNTRMDAGDFALQRMAKKLDKADHFELTQV
                     AVFFGEPDKTVPDPYFDGKGPERAGCNYCGGCMLGCRFNAKNTLDKNYLHLAQNLGVK
                     IQAETEAIAVTPLNDNNGNDGYRITIRSSTRWFSKPKTVTTKGVIFAGGVMGTVPLLL
                     KLKKEHLPRLSDRIGCQARTNSESLLGVTTFDKNTNFSTGIAISSILHTDEHSHIEPV
                     RYPAGSGAWRILGSPMTAGKNWVTRLSKALWDILRHPIRNARIYFVSDWARRTQILLF
                     MQTINSTLRFSLGSWGMKSTMEQGVAPTVFMPEAQSLAKHFAEETNGKPVALLTETLL
                     GIPTTAHILGGCVMGRDASEGVIDKDHRVFGYENMYVCDGSAISANPGVNPSLTITAL
                     TERAMSKIPNASDILNSL"
     gene            11375..11503
                     /locus_tag="PK937_08620"
     CDS             11375..11503
                     /locus_tag="PK937_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32673.1"
                     /translation="MSKERKQLTRDRKIETVRLSYQRDSVVEFAGELGLRPALIYR"
     gene            complement(12154..12291)
                     /locus_tag="PK937_08625"
     CDS             complement(12154..12291)
                     /locus_tag="PK937_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32674.1"
                     /translation="MKEYIRLSEREHEEISGMPAQNFFFRQMAMRLNRHREGCCHNRY
                     S"
     gene            12316..12819
                     /locus_tag="PK937_08630"
     CDS             12316..12819
                     /locus_tag="PK937_08630"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HNH32675.1"
                     /translation="MTVIILYRANDVFDAAGLKKPSIEILDERFLQELKDLPQKNLAV
                     KLLKKLLKDEIKKRTKINLVENKKFSEMLEDAVKRYHNGMIDTVEFLEKVLIPFAEHI
                     KAADKRGEALGLDYREYAFYTALEVNNSAVSILGDATLKSIAVELLKSVRGSTTIDWT
                     IKENSAS"
     gene            12845..12952
                     /locus_tag="PK937_08635"
     CDS             12845..12952
                     /locus_tag="PK937_08635"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32676.1"
                     /translation="MRKFGYPPDLQEKAVETVVLQAKLLADEIALSNKF"
     gene            13120..13689
                     /locus_tag="PK937_08640"
     CDS             13120..13689
                     /locus_tag="PK937_08640"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32677.1"
                     /translation="MYYVGLATDLRTRVKQHLKDRHSDKWDTFSLYLIKNVHFLKELE
                     SLILHITHPKGNVQRGRFAKSLDLLPVLDGMMTARDRRKRESILDTAARRKRLPASGK
                     SVRPRNLENDPPLVLKPGMKQQAVYKGVLYGARVDQSGRIRYGGKLFPSPSTAGSFIR
                     SGKSTNGWVFWKYQTPDGHWRYIDELRNR"
     gene            13722..15194
                     /locus_tag="PK937_08645"
     CDS             13722..15194
                     /locus_tag="PK937_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32678.1"
                     /translation="MEFVLAHYIHRTIPMKFLIYGVLFCCFGVLYADTLSGSEVDSAV
                     IRFRQLVREHRIIQLSRLIKYPLRRTSPIPDIQNPYAFIRYFPVLFDSSITKRLNASG
                     NDDLVWRTDHYEMVDGRIWLDSNGYIVAIPYVSSHEEHLISQIKAEACAADITDSVFA
                     RRTRLFWDADAYIIRVDETAEGLRYSSWNRGKSVTQSPDLMLWRGSVSNDSSRFIFIQ
                     GVWRYVLTTEFSPDEAKPQKHFLEVWKADTLKMRLPCEELAHAQQISRFCETIHHIRN
                     DRIVTLSRGVRFPFGSLMDMLPPEENPDEFILSYPLMVDSMVHRRFNNYHPVSIAHKY
                     GYMWEDLFFDDNCRLTLLGYKAALADSIFLWLQREVTDALYSELREFGSHDMMLISDN
                     HVLRIDWMDEQRGWRYVAWRKGKAISDVPDIILYGGTAEYHGNMGSKTLTFTNGTWSY
                     IIDYWVNDYLNPTLIIKNNAQEIINTKCQRITFSDCKLYH"
     gene            complement(15296..15847)
                     /locus_tag="PK937_08650"
     CDS             complement(15296..15847)
                     /locus_tag="PK937_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH32679.1"
                     /translation="MNDFFEQTQARYTAILERLDRDIRSFSDAQIHWKADANTWSIAE
                     ALQHIITADTAYLEPMHRALQGAERTASPVAYRPSFFARLFIYSLRPGRFRYKAPAVF
                     KPESSRISHTIRQNFADHIRTQMQALQAWRSYDINRLKMTSPANAMVRISLGEALDVM
                     ITHTERHMAQVDRILQHPQFPTL"
     gene            16122..16667
                     /locus_tag="PK937_08655"
     CDS             16122..16667
                     /locus_tag="PK937_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32680.1"
                     /translation="MKKILNIYFLFGALYTLAYAGDGVTNEFVATAGWGAPVGIGLEF
                     EKYIVPAFNLNMGAGVTVTGVKLSVGTKYLMAADKDVSPYIGLNFSSSSGIRRLNINV
                     DTDTAKYKINAAKILTPRAGLKLKRGFANFYLNVGYGIVVDGGGNKYRSGSTDTGISD
                     MADLLSAGGVEISGSICFPIK"
     gene            16758..17357
                     /locus_tag="PK937_08660"
     CDS             16758..17357
                     /locus_tag="PK937_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32681.1"
                     /translation="MRVMLTVFVILLVLSDILSAQVSYLQNNESNAVALSAGASIGSK
                     NNKSRALGFETGQRKFVFGVSYSETKPQSEDKYDAVSAHIGTTFPHQIDGMNLSLRLG
                     YLGSSKIKIPAKHVVFSPSVFLNIAHKITADSFIKIIPEAGVSRLFVFEEQYEGDLVS
                     SVGVTILYNRPTMAFGLGLSRNWSSGPVVNLLSFSLITD"
     gene            17473..18834
                     /locus_tag="PK937_08665"
     CDS             17473..18834
                     /locus_tag="PK937_08665"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNH32682.1"
                     /translation="MNRIQLDYTYVMPFLKEEEIFGMQAKVNDVHKMIEQRTGAGNDF
                     LGWLDLASKFPSALVEDIAKTAEDIRKTSDILITIGIGGSYLGAKAVIDALTHSFNNN
                     IPHKTPQVYFAGQNMSGRYLRDLTEVIQNKRVAVNVISKSGTTTEPALALRYFRKIMS
                     DAMSKMQVSQRIFATTDAARGSLKKLAEKEGYKCYVIPDDVGGRYSVLTPVGLLPIAV
                     AGVDIRALLQGAADMEKILSNPDLKSNPAYVYAVIRNLMYNKGKAIEILANFEPSLHY
                     VSEWWKQLYGESEGKDHKSIYPASVDFSTDLHSMGQWVQQGTRNIYETFLWVDKAQSD
                     TSVPDLEGDVDGFNFLTGQTFEYVNKKAYEGVTLAHRDGEVPNMTVHLPELNAYYLGQ
                     MIFFFEKACAMSGYLLGVNPFDQPGVEAYKTNMFALLGKKGYEEKSKELNAVLGQMQR
                     KKI"
     gene            18840..19163
                     /locus_tag="PK937_08670"
     CDS             18840..19163
                     /locus_tag="PK937_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32683.1"
                     /translation="MMTRSEALRAILEKDPGDSFSRYALAMELVGAQRWAEAIAEFTD
                     LQRRDPSYVALYYQLGKCYEQNNQPQDAIAAYTEGIRVARQANNMHAVSELYAAKEEL
                     EDTLA"
     gene            19171..20760
                     /gene="rsmB"
                     /locus_tag="PK937_08675"
     CDS             19171..20760
                     /gene="rsmB"
                     /locus_tag="PK937_08675"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNH32684.1"
                     /translation="MPFSKKPGARSGAKRTSDKRTSDKRTSDKRTSENRTSEKRTFAG
                     KERGERYSDERPKPRRRPKYYQPRTFMGANSETEERRLAEWTGQRIALEVLHAFEETQ
                     ERADILLRTHIQRHLGILDSRDREQIARTALNVIRYKLRLDYVVSKTLKRNAEELEPL
                     IRCIYRLAAFHIVHLGHSVERTQAIVNFIVPKSYGNQKENLRNFISYLKKYHTEISFP
                     NPRKQPVEALAITYSHPSWLIEKWSKTYGTESTVRMCEFDNQETPLVLRINTLKATEA
                     DVFKKLKAEGIIPKKTAYAQYGLIPGEVHDIFNRKIFKDGWVEVQAESSQIFCEWVNP
                     ASEDRVLDACAGGGGKSLALSMLMHNTGHIVAADINAGALEQLKKRATRAGCKNIEVI
                     ENDALAALNQRGTWYDKIIIDAPCSGLGVLQRNPDMRWRVTQDNIEKLVNEQRTLLEN
                     YAPLVKPGGMLIYATCTLSEEENENVIEAFLSAHPEFELQKNHDARWKKFISGKGYFC
                     ITPFEHNISGFFSGKLIRRKN"
     gene            20779..>21641
                     /locus_tag="PK937_08680"
     CDS             20779..>21641
                     /locus_tag="PK937_08680"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNH32685.1"
                     /translation="MKILVLGAGWMGRAVVFDLLRNHHMERIIVADANAQALKETEAF
                     VKAENDLRFLAKKINVEDEEALKKLMSQVDTVVSAVTYKYNYKLARLAVQCGCNFCDL
                     GGNNTIVAQELSLHDKAKKKNITIIPDCGLAPGMVSVLVADGIGQMDETTDVQIRVGG
                     LPLEPRPPMNYKLVFSVHGLINEYVEPCVKIQSGEVVTVDPMVDVEELEFPAPFGKLE
                     AFNTSGGTSTLPQTYLGKVKNLDYKTIRYPGHAAQFKLLMDLGLTESKPVKVGNDTVV
                     PRDLLAQNLTKV"
BASE COUNT         5836 a         4740 c         5316 g         5749 t
ORIGIN      
        1 atttattaaa aatacataaa gaaaggttac gtcattggaa caactcattt atctgccggt
       61 tttgatttta gccgtcgtcg tacacgaatg tgcgcacgga cttgtagcta aatggtgcgg
      121 agacgatacg gcctatcgcg aagggcgtat tacactcaat ccgatcagcc atgttgattt
      181 atggggaacg atcatcgtgc ctttggtact ggcattgagc agtgcaggac tgatgtttgg
      241 ttgggccaaa cccgtgcccg taaatcctaa caattttcgt cactttcgca gagacgatat
      301 tttagtttcg atggccggac ccgcatctaa ttttatcatc gcattcatat cggcgctttt
      361 catcgttttg ttgtccttag tatggaaggc cgcatcaccg gaatccgcgt caagtatgca
      421 attcggtgaa tttgctttgg aacttttcaa acatagcata cagatcaatt tgtatcttgc
      481 tttgcttaat ctaattccga tacctccact ggatgggtca catgtcattg caaacctgtt
      541 gccgcctcat ctcggcgaac aatatcggag cttaggtttt attggaatat ttattttgat
      601 tgttatgatt agtattggtg tgatcaatgc agcgattgac ccattcatcg agtatttgat
      661 tgggttgctc aacgcgttta tcgttatgtt ggcgagttag attgcatgca acttcagtat
      721 gaatcacgta cgctccattt gcggcatccg tttattattt cgggttcgca gtattcggaa
      781 aaagttgttt ttacggcgac gctgatgcat gaaggtatcg taggacgcgg cgaatcttcg
      841 ccatcgcgtt actatggtga accgccggaa tccgtacaga cgatgttatc cgacaccgca
      901 cgatggatcg gtgacatatc ctcgcccgcg gatattcaaa ctttgcacga aacgcttctt
      961 caaaaatatc cgcatcagcc gtcagcccgc tcgtgtgtga tcatggcggc ctacgattgg
     1021 cttgcacgat cctgtcagat gccgctgtac cggctttggg gattaaatcc tcataaggcg
     1081 tgccgcacat catttactat cggtatggac gaacccgatg tgttgaccga aaaagtcaaa
     1141 gaagcggagg cgtaccctgt tttgaaaata aaactgggga acggcgatga agattacacc
     1201 atcatgcaga ccattcgacg ctgtaccgat aaaacgattc gtgtagacgc caacgaaggc
     1261 tggcaacgtg acgaagccat cgaaaaaatc acctggcttg ccactcaaaa cgtagagtac
     1321 atcgaacagc ctctgccgaa agaatcgctg gaggatatgg tatggctcaa agcgcgttca
     1381 ccattacctc tgattgccga tgaaaacgtc atgacgtcgc gggatatacc gggattgcat
     1441 cacgcatttc acgggatcaa tctcaaattg gacaaatgcg gcggaatgtg ggaggcttta
     1501 cgtatgatac aaacggcccg cgctttggat atgaaaataa tgatcggctg tatggtcagc
     1561 acttcgctgg ccatgacggc cgcggcgcat ctgacaccgc tgacggacga agccgatctg
     1621 gacggccatc ttttgattcg cgacgatcct tttcgtggtg taacgatcaa tcatcatggt
     1681 caacttattt taccggaaag cgatgggatc ggcgtcacag aacaaggtat tcattagccg
     1741 tgttaccgcg attacccaag catcgtcatt tttcatacac accgcgccgg tataaaccgg
     1801 aagcggaacc gacgtctcgt aaatctatcc gctttcaacg taatgatacc cgcaaaccct
     1861 cgatttttcg atggctatgg gtagtactta ttatactttc gctttggttg attttaaaaa
     1921 atcttgctat acaatagcaa aaatgttact ttcacgcgtt tatcagaatt ttttttgtga
     1981 ttcttcttcg gagtttatgg aatgatacaa ataggcgaga ttctcaaaca agtacctttc
     2041 tttcgctcgc taggtcgcga tagcattgat tttataaccg aaaagctcaa gttcaaacaa
     2101 tacaagaacg gaactcccat ttgtaaaatc ggcgatcccg gtgataaaat gtggatcgtt
     2161 ttgagcggta aagtcgctgt cagcgcagcg aacggtgaag ttttagcaca aatcgggcaa
     2221 ggcaattatt tcggtgaaat ggcgttgctc accggtgaac cgcgttcggc cactgtttcg
     2281 gcggcggatg atacggaaac atttattttg gacaaggatg actttgatgt cattttagaa
     2341 aagtatcctt cgatctccat tgccatgagc aaaatcatga gccagcgtct ccgcgagacg
     2401 aacgccgtgg tttccgaaaa agccaaagag cttaaatcgc aagcgcccgc gggaccatcc
     2461 ggcagtctgc gcgataaaca tatcaccgaa gtcctggctt tttgcgaagc taattcgctt
     2521 accggtgcac tcgaagtatc caatgcgggt caaacggccg tgatcgaatt tgaaaaaggc
     2581 gttttgcaaa cgattaagtt tggcacgatg ggtgaagacg aggcaatgga tacgctgatg
     2641 ggatgggaag acggtacatt cgtcgtcaag ccgcgtctga ttgcgttgga cggactgggc
     2701 tccggcagtt caacggaaac acccaaacat gaaagcaaaa aaaccggttc aatactcgtg
     2761 atcaatcaca gtatggtggt tcgcaaagtt cttgaaaaaa atctaagcag tcttggtcac
     2821 acggtcaaaa cggcggacgg gattaccgac gggatcggcg cgttgcgaac cgtcaaacct
     2881 aatgtgatta tttcagattt caaatatgat gaaatttccg gatacgattt ttgtatgcaa
     2941 atccgtgaga cggctaatta tttggatata ccgtttattt tcatcaccga ttcgagtacg
     3001 gattcggcgg ttgtgaacca attaaaaagc gtgtctaatt cggccgtggt ggatgcgcaa
     3061 gacgtttcgc atatcgccaa taccattgaa aaaatgctgc cttagttcat aaggcgttaa
     3121 aattaagaag ctgtatccgg cagttgtttg agatcatttc gtgccattgc atacattctg
     3181 ttataactac aataatggaa actcgtatca acagacacga ctgagtcgaa tgccagatac
     3241 cgcttctttt tttatgcaaa tttttgacac gtcgtatttt ttattatttc aggctctcta
     3301 ttaatttttt agcggcttcg tttccgggtt cgatttccaa aactttttta gcataggatt
     3361 ttgccacatc aatgtgtttg gcttcttgat gaagtgtggc gagcgttata agcgttcgta
     3421 cagaattggg gaaatgctca ttattgagtt ccaccaaagc gatagcatcg gaagggtcgg
     3481 ttgctttttt gatgcgtgaa gccgtacgca acagcacacc ttctctgaaa tcatacgtaa
     3541 aacttccata atatgtgctg cgcagtgagc ggtattggtt tttgaccgaa tcaatacctg
     3601 atgtcgcata cgtcgaagcg aggatatctt caatgcgttc cggcagcggt gcgccacgat
     3661 ggcatgtgac gcaagtgaca tcgagtggtt tggatttgtt ttcattcgca tgggcgatat
     3721 aagtttcgtt gatgactttg accatcgtca tcattttccg tgccgtattt ttagtttctt
     3781 tggcatcgga gacaaaatca aacgtcgaga gcgatttgtt atcttcaccg acatggcaat
     3841 acacgcaacg aacaccaagc ccattgataa attcagacat ggtttttctt aattcggtgc
     3901 cacgtgtatt tgacggaaga acctgcaggt tttttgatga gtctggccat tgccaatttt
     3961 gtgattgggc atacgatgcg acagataaaa aagcaatcat taagagacta cgcatagaag
     4021 gctcctgaag ttaagtggta gggtataagg gtataatagg tgtaatcagt tcctaatacg
     4081 taattaggac ctgcaggttg cacgggcggg ttcttttctg gagttttttt tagacaattc
     4141 tgacgccatg ttacgttgtg cgatccactg gccgtacatt tttccggctg aaatgaattt
     4201 atgtaatttt tcgattatgc gttccgaaat atggcctggc gaagatgatc gaagcggtgt
     4261 gccgggcagc ggcataaacg tatgcccgtg aattcgagca cccatatcgg ttaatcgttc
     4321 cgccaattgc agcgaagccg tgatatcctc ttctgtttca tcagggaatc cgaaaataaa
     4381 atccacattg ggtaaaaaac cggcttcgac ggagtaacgc acggcctgtt cgatgcagga
     4441 gacgtcgtgc ccgcgatgca tgcggtcgag catattttgt gaaccggatt gagcgcccat
     4501 gagcagattg tcattgtgca catatttttt taatacccgg aggatatccg gcgtcacgtg
     4561 ttccggcctt acctccgaag gaaacgttcc aaaataaata cgtccgcgat cacccagaac
     4621 ctgtcgcacg cctttcagta acgcctcaat ggcctcgagt ttgaccgatg catcatcgga
     4681 accgtaggac aaggatgtcg gtgtaataaa ccgaatgtcc catgcgccgt tttctttcat
     4741 aatctgaaca taacgcatta ccgaagcgac ggaacgatgg cgaaagcgcg ctttcatcat
     4801 aaagggggtc tggcaaaaac tacatgcata aatgcatccg cgggttatct caataggatt
     4861 gacacgtacc catcggtctg caaacggcgg gaaatcatcg agctgtatca acggcggcgg
     4921 cggattacgt ttccatgttt ctccgtggat ataagccaat ccgtgtgtgg tgtagacatc
     4981 atctccattt aataaacgcc gcagcaattc gacgatgatc atttcaccct cgcccatcgc
     5041 gacccaatca aatcccatac gaagcgtggc gtgcggctcg gagctggcat gcacgcctcc
     5101 cgcgaggtgt gttatgtcgg atgtatgcgg ctgttcccga aaccaatgga ggtcttcttg
     5161 tacgccggta ctatgcgtgg aataaaaaga ccaacttacg acgacgcggt ggccttgatt
     5221 atacgccgat agagctttgg aatacagttc gtcgcgtcga aaagctaatg cgatatgcag
     5281 gcgcggatct tcaatgccct gctgtacggc cccgataaga gtattcagcg cgtacgtatt
     5341 gacacctttg taatatataa taagtgtgag cgcttgcata aaatgatatt cataaatttt
     5401 tagacgacaa acaatgttaa aaactatacc gagctattgt tttatttagt caaaataatg
     5461 atattgttat aggttttcac atcgcgggtt ctaatttttt tatataaaat tatgttgcgt
     5521 tttctcgtct ctttgatctt aatctttgtg ctaaagccaa ccgcgcacgt gttggcgctt
     5581 gatcctcagc ggacgctgtc gcaatatgtt cagtcttcgt ggaccacgga gacaggcctt
     5641 cctcaaaaca caattcacgc tattgcgcaa tcgcacgaag gttatatttt cttcgggact
     5701 tacgaaggct tagtacgatt cgacggtgta cattttacgg tatttgatcg gcgtaatacg
     5761 cctcagcttt tgaataacgg tattttatct ttgttgtata cgcgcgatag cacattgtgg
     5821 atcggtacca atggcggcgg tttgacccgt ttgcatcgcg gactgtttac gacgatagat
     5881 atcaattccg ggctcccgca tcgcatcgtg acggctatag ccgaaggaga agacggaacc
     5941 ttatggatcg gcacggcggg tggcggattg gcggcgtact acaagggaga gatcaaagtg
     6001 atttctaccg atcaaggcct tactcagctt acggttaatg ctctgtgtgt gactaaaagc
     6061 ggcatgttgt ggattggaac caacggagga ttattcagta taccgggcag tcgtttttcc
     6121 gatatatcgg gttataccgt tacgcggcac tccgatccga acggttcctc attgacgggt
     6181 attcaagcta tcacggaaag cccttcgggc tatatgctga tcggtaccaa tggttccggc
     6241 gtctatacgt atcggcatgg tgcatggtct tctatcactt tacgtggtgc gctgaccgtc
     6301 ggagcggtgt atgccattcg ttccgatccc gacggctcgg tttggatcgg taccagcgga
     6361 agcggattaa agcgtattgc attcattcaa atcaacagcg aaggtttgcc gcagtcctat
     6421 acggtagacg aagcgtttac caatcaatcg gtctggagtt tattcgtaga tcgcgacggg
     6481 catatctgga tcggtaccaa tggcggattg catcgcctga tgaacggccc atttacgacc
     6541 tatactacgc aagacgggtt gcccgatgat tacattcgcg ccgttactat ggatcgggat
     6601 aatgacttgt ggtttggttc gcgcggcgga ttgacacgca tgcaatgggt gcaaggtaag
     6661 ccggtatggc gcacgtttac gccgaaagac ggactttcga atatttccat tttgtcgctt
     6721 ttttccgagc ccgatcaatt gtgggtcgga acgtacggcg gcggactcaa tcgccgttcc
     6781 ggtgaacaaa cgcaagtata tacagagagt cacggtttgg gaagtaatgt catccgagcg
     6841 attgcaagag atgtgtatgg taatctttgg gtaggcacat ccggaggaat atctatggcg
     6901 ttagcgccgg acttgaaaca tgcacagccg cacaatatgc tcttccgaaa attctcttca
     6961 cgcgacggtt tatccaatga tcacgtatat acgattttgc cggatgcgga cggcagcgtt
     7021 tgggtcggta cgatttccgg tctcaaccgt atcagcgaag gtcgtattca aacatggcat
     7081 agcggcgctt tttcatcgaa tatattggcg atgttcctcg agggggattc cgttttatgg
     7141 gtcggtacgg acggcgggtt ggtgcgggtc aataaatcat ggctgctcaa aaacacaaaa
     7201 attccgacac aaaataaaac gagtgaattg acaactttaa cagtgaatga gggattacac
     7261 tccgataaaa tttttacgat actatttaaa tcgccgtgcg atctgtggat gagctccaac
     7321 cgcggcgttt ttatgatatg caaagatcag gtaaccgcct ttattcgcgg tgaaatcccc
     7381 gcggttcaca gttcatacta cggtattgag gacggcctca aaacgattca atgcaacggc
     7441 gcttcgcaat cagcgggtat tatcacttcg gatggtacga tgtggtttgc gacggccggc
     7501 ggcctgagca gcatcaaaac ggaatcgttg accgaaaatg cgcaggtttt acttccgata
     7561 gccgaatata tcatcgccga tcaaaaacag tactggattc ccgctgacgc gtcgtctatt
     7621 gatttacccc cgggcacgca acatgttgag ttgcattata cggccttgga ttttgctgaa
     7681 gccagtaaaa tccgttttca atatcgtttg gatggattgg atgaaaattg gcatctggcc
     7741 ggatcgcgtc gcgtggcgta ttacaccaat gtgcctccgg gttcctatgt gtttgaggtc
     7801 aaagccgcgt cggctatggg taattggtct tccaagccga cggcgattcg tatccacgtc
     7861 caagcctttt tttatcagac actgtggttt tatgcattgt gcgccgtgat ggccgccgta
     7921 tgcgcatggg gattgtatca ctggcgtgtg cgcgttttga ccacaagaca acgcgaattg
     7981 caaaaaataa taactcaacg tacgacagaa ctaaaagaat cgcaggcgca attagttcac
     8041 tctgaaaaaa tggcggccct cggtcgtatg gtgggcggta ttgcgcatga ggttaataat
     8101 cctctgacct tcatttacgg caaccttcat tttttacatg cccatcaagc gacgtttcgc
     8161 caattactca aactgatcga acagggtgga acagcggaac aaaatcaaaa tatgctcaag
     8221 attttatcag atgccggtta tgcctcgctg gatgaagcct tgaatgaaac gattacacta
     8281 attgatcagt ttaagcacgg tgcagatcgt attcgtaata tcgtaatcaa ccttaaacat
     8341 tttgccagcg tcgaatcatc ggaagatgag tccgtagatg tgcatgaagg gcttgaggcg
     8401 acgatcaaac ttctgcaagc gcagaaccaa ctgcccatcg aatactataa agattttacg
     8461 gacattccca aagtgcacgg attgagcgga gaaatcaatc aggtgtttat gaatgtattg
     8521 atgaatgccg tacaagcgat cgaggggcag tggcgcaaag gtgttacgcg atccggtcat
     8581 attcgcattc gcacggagtc atgtcagctt tatgccaaag aagcgattcg catacgcatt
     8641 cgcgatgatg gagagggtat atcggaagag catcggtcta aaattttcga tccttttttc
     8701 acgacacgta cggtcggcaa aggaatgggt ttgggactca ccgtttgtta tgggattatc
     8761 gaacgccata agggaaggat atacctcaat ccggatcaac gcgaagaaac cgagatcatc
     8821 atcgagctcc ccgtcaaaac cgaatagaat tgaatttcga tttctctctt aacggctttt
     8881 tttcaccgat ccagtgcgaa ggcggccgta atgctgatga aaatggtaag ccactccgcc
     8941 gtcaattttt tccatgttga gcttgtatca gccattttga gaattctcca ttatattacg
     9001 attacggtag tttagagccg cgagcgctcc accaaggatg gatttcatat atcaatcgtc
     9061 cggcctgtac ggcggggtct ttctccactt cggttttagc ttcttccatc gttttgacgg
     9121 tcagaatgag aatgccgcgc cagtcaccgt tatcgccgaa cggtcctgca atggcgagtt
     9181 taccttcgtc ggccatgcgc gtaatattac ccatatgccc ttcctgcaat ttcatagccg
     9241 tcaccgaatc ttgactacga ttaggacctt ttttgagcat cacaaaaaaa tactcacgca
     9301 tttcgtactt aggtttgttt tcctgagcaa aactatttac cgaaaaaatc attgcgacca
     9361 acacgatcca tttcatagga aatcctcatt tagattatgt taaatgtatt tatagtgaat
     9421 ttaatatgtc cgatgcattc gggattttac tcatggcgcg ttcggtcaac gctgtgatcg
     9481 taagcgacgg gttgacaccg ggattggcgg agatcgcaga accgtcacac acgtacatgt
     9541 tttcgtatcc aaaaacacga tgatctttgt caataacgcc ttcggaggca tctcggccca
     9601 tgacacatcc gcctaatatg tgtgctgtgg tcggaatgcc gagcaatgtt tccgtaagca
     9661 gtgcgaccgg tttaccgttg gtttcttcgg caaaatgttt tgccagtgat tgcgcttcgg
     9721 gcatgaatac ggtaggcgca acgccttgtt ccattgtgga tttcataccc catgatccga
     9781 gagaaaagcg cagtgtgcta ttgatcgtct gcataaaaag aaggatctgc gtgcgccgtg
     9841 cccaatctga aacgaaatat atccgcgcat tgcgtatggg atggcgaagt atatcccaca
     9901 aggctttgga taaacgtgta acccagtttt ttccggcggt catgggtgaa ccaagtatcc
     9961 tccatgcacc ggaacccgcc gggtaccgta ccggttcgat atggctgtgc tcatccgtat
    10021 gtaaaatgga actgatagct atgcctgtgg aaaaattggt atttttatca aatgtcgtta
    10081 ctccaagtaa gctttcggaa ttggtgcgag cctgacatcc aatgcgatcc gacaagcgcg
    10141 gtaagtgttc ttttttcagt ttaagcaata aaggtaccgt gcccataacg ccaccggcga
    10201 aaatgacacc ttttgtggtt acggtttttg gtttagaaaa ccaccgggtc gaagagcgta
    10261 tcgtgatgcg ataaccgtcg ttgccgttgt tatcgttcag aggagtgacg gctatcgctt
    10321 ccgtttccgc ttggatcttc acgccgagat tctgagcgag atgtaaataa tttttatcta
    10381 atgtattttt agcgttgaat cggcatccga gcatacatcc gccgcaatag ttgcagcctg
    10441 cgcgttccgg gccttttccg tcaaaataag ggtctggcac ggttttgtca ggttcgccga
    10501 aaaaaacggc cacttgcgtt aattcgaaat ggtcggcttt atccagtttc ttagccatcc
    10561 gttgaagagc aaagtcgccc gcgtccatgc gtgtattacg tgttgcgccg agcatacgaa
    10621 gcgccagcgg ataaaacggt tttaattctt ctttccaatc ggctaaatga gaccatgaag
    10681 gcgaagtgaa aaagttttct ttgggtaccg gaagggtatt ggcataaacc agcgacccgc
    10741 cgcctacacc gacaccggag agcacggtga cgtgacgaaa aaaagtcagt ttgaataatc
    10801 caaaacatcg cagtagcgga atccacatcc accgtcgcac attccagttg gtcttaggaa
    10861 agtcgcttgc gcgaaggtgt tttccctttt cgattacaag tacattatat cctttttcgg
    10921 ccagtcgtaa cgccgacacc gagccgccaa atccgcttcc gataacgaca aaatctacat
    10981 cgtggcacat atcattgtat ccggtgtgat tccttgcaag tcatagtatg actaagttat
    11041 tcaacgtgat aatgaatgta aagaatgcaa tgcaatagtg aaagaaacat tctgaattct
    11101 gcgatagaaa aagatgaatt tgattaacat aagtcatagt tggttttgcg gtttatggag
    11161 tgagagttaa gtgatggtag aacgacattg agacacgtcg tcatgggaat ctatgggtgg
    11221 ttctcaagca gatgggaaag aacaaaaaaa gtgcgttaga taagattggg cggatgaagt
    11281 tgttcgagat gttgaaccgc agttagtttg gaattgttac atgtgaatgg acagttgatt
    11341 aaggtatatt gaacaaacca aatgagctct aggcatgagc aaggaaagaa agcaacttac
    11401 gcgcgataga aagattgaaa ccgtccgatt gagttatcag cgtgacagtg tagttgagtt
    11461 tgccggtgag ttaggactgc gcccagcgtt gatatatcgc tagcggagtg aatttgcggc
    11521 tactgacgag aaaagttttc caggtaatgg gaatggccag tcaagtgaag agtaggtcac
    11581 acgtcttaag cgtgaactgg caggtattcg catggagtgc ggcatactaa aaaaaactgt
    11641 gcccatcttc agtgagtatc aatgataaaa tatcagatca ttgataccca ttgcgatcaa
    11701 tattttgtgg agaagatgtg tcaagtattg gaagtatcgc gcaacagtta ttaccaccag
    11761 cttacacata aaaaaaggcc gccttgggcc aggcaaacga gagtattttg atggatttat
    11821 acgccaaata tggcttgtga gcaaggaaag atatgaagca cgagagattc gcatggaact
    11881 cataaaacaa ggcctccgag ccggtcgtgc tcgggttcaa ctccgtatga aagccatgaa
    11941 tatacgatgt acctaccgac cgaaattccg agtaacgacc gatagcaaac atcatctttc
    12001 tattgcggaa aacctacccg cgcagagatt taattcgagc agctttccca agtgcgaatc
    12061 agtgatatga cctacgtgcg tcatctttcc acgtggattt atttgaccat tgttataggt
    12121 ttagtggatc ggctgatcat cggatgatat ttatcagctg tatctattgt gacaacagcc
    12181 ttcacgatgg cgattcaggc gcatagccat ttggcgaaag aagaagtttt gagccggcat
    12241 gccgctgatt tcttcatgtt cgcgttcact taatcggatg tattctttca taagaatcta
    12301 agcaagtaaa gcctgatgac agtgattata ttatataggg ctaatgatgt ttttgatgca
    12361 gccggtttga aaaagccaag cattgaaatt ttggacgaac gctttttgca agaactaaaa
    12421 gacttgccac aaaagaattt ggcagttaaa ttattaaaga aactactcaa agacgaaata
    12481 aagaagcgga caaaaatcaa tttggttgag aacaagaagt tttctgaaat gttggaagat
    12541 gctgtcaagc gctaccacaa cggcatgatt gacacggttg agtttttaga aaaagtatta
    12601 ataccgtttg ccgaacatat aaaagcggcc gataagcgtg gtgaagcatt aggtttggat
    12661 tacagagaat atgcgtttta caccgccttg gaagtaaaca acagcgccgt ttctattcta
    12721 ggtgatgcga cattaaaaag tattgctgtt gagctgctca aatccgttcg tggcagtacc
    12781 acgattgatt ggaccatcaa agaaaacagt gcaagctgat ttgcgaagaa atatcaggcg
    12841 tatattgcgt aaattcggat atccgcccga tctgcaagaa aaagcggtag aaacggtagt
    12901 attacaagcc aaattgctgg ctgatgaaat cgcgttatcc aataaatttt aattttcata
    12961 ttggcttctc gtatacctat gcccacagtt aaaggacaag aattgattac aggcaatctg
    13021 gagcggattt ccagtaaagt atttgatcac taccatgacg agatcaccga tctgatcggc
    13081 aaaagtcacg gggtgtaagc gttatacaaa gaggatcgtt tgtactacgt cggtctggcg
    13141 accgatctgc gcacacgggt caagcaacat ctgaaagatc gccattccga taagtgggat
    13201 acctttagtt tgtacctcat caaaaatgta cactttctca aagaactgga gtcgctcatc
    13261 ctgcatatca cccatcccaa aggcaatgtc cagcgcggac ggtttgccaa gtcgcttgat
    13321 ctgttgccgg ttttggacgg tatgatgacg gcgcgtgacc gacggaagcg cgaatccata
    13381 ttagataccg ccgcccggcg aaagcgcctg cccgcgtccg gtaaatccgt acgtcctcgc
    13441 aatttggaaa atgacccgcc acttgtgctc aaaccgggta tgaaacagca ggccgtttat
    13501 aaaggcgtgc tgtatggcgc acgcgtggat caaagcggtc gaatacgtta tggcggaaaa
    13561 ctgtttcctt cgccatccac ggccggttcg tttattcgca gcggcaaaag taccaacggg
    13621 tgggtgtttt ggaagtatca aacaccggac ggccactggc gatacattga tgaattaaga
    13681 aaccgttaga aaatcagata attatctctt atgcatacat catggaattc gttctcgcgc
    13741 attacatcca tcgcactatc cctatgaaat ttttgattta cggtgtatta ttttgttgtt
    13801 tcggtgtttt atatgccgat acgttatcgg gttcggaagt ggatagcgct gtaatccgat
    13861 ttagacaatt ggtacgcgag catcgtatta ttcagttgtc ccggcttata aaatacccat
    13921 tgcgacgcac atctccgata cccgatattc aaaacccgta tgcttttatt cgttatttcc
    13981 ctgttttgtt tgatagttcg atcacaaagc gtcttaatgc ttccggtaac gacgacttag
    14041 tttggcgaac ggatcattat gaaatggttg acgggaggat ttggctggat agcaatggat
    14101 atatcgtagc cataccttat gtgtcatcac atgaagaaca tttgatctcc caaatcaaag
    14161 ctgaggcatg tgccgccgat atcacagata gcgtgttcgc tagacggacg aggctttttt
    14221 gggatgcgga tgcttatatt attcgtgttg atgaaacggc ggaaggactt cgctattcgt
    14281 catggaatcg cggaaaaagc gtaacccaat cacctgatct tatgctttgg cgcggtagcg
    14341 tttcaaacga ttccagtcgg ttcatcttca tccaaggcgt ttggcgatat gtacttacaa
    14401 ccgaattctc gcccgatgaa gctaaaccgc aaaagcattt tctagaagtt tggaaagccg
    14461 atactctcaa aatgcgattg ccttgtgagg aactcgcgca tgcgcagcag atcagtcgtt
    14521 tttgtgagac gatccatcat atacgtaatg atcgcattgt gacattgagt cgtggcgtgc
    14581 gtttcccttt tggatcatta atggatatgc tgcccccgga ggaaaatccc gatgaattta
    14641 tattgagtta tccgttaatg gttgattcca tggttcatcg gcgttttaat aactatcatc
    14701 ctgtctcgat agcgcacaaa tacggatata tgtgggaaga tttgtttttt gatgataatt
    14761 gtaggcttac tctactcgga tacaaggcgg cacttgctga ttccatattt ttatggctcc
    14821 aacgtgaagt gacggacgcg ctctattccg agcttcggga atttggtagc cacgacatga
    14881 tgttgatttc agataatcac gtacttcgaa ttgattggat ggacgaacag agaggatggc
    14941 ggtatgtggc gtggcgtaaa ggtaaagcta tttcagatgt tccagacatt atactatatg
    15001 gtggcacggc tgagtatcat ggtaatatgg gcagtaagac gctcacgttt accaacggca
    15061 cgtggtcata catcattgac tattgggtaa acgactatct gaatccaacg cttataatca
    15121 aaaataatgc gcaagaaatt attaatacaa aatgccaacg tattacattc tcagattgca
    15181 aattgtatca ttaatggact aaacgggaaa agcgccaagg catagacaaa aaaccaaatc
    15241 gcacgcaccc actcaatcaa tcccgttatt gttcagccgc acgtaaatcc aaatttcata
    15301 atgtcggaaa ctggggatgc tgtaggatgc gatccacctg cgccatgtgg cgttcggtat
    15361 gggtgatcat cacatccagc gcttcaccga gactgatacg caccatcgca ttggcgggtg
    15421 atgtcatttt gaggcgattg atgtcgtacg aacgccatgc ctgtaacgcc tgcatctgtg
    15481 tgcggatgtg atcggcgaaa ttttgacgaa tggtatggct tatgcggctt gattccggtt
    15541 tgaaaaccgc aggggctttg tatcggaaac gacccggcct cagggaatag ataaataaac
    15601 gtgcaaaaaa actcggacgg taggcgaccg gcgaagccgt acgttcggcg ccttgcaaag
    15661 cacggtgcat aggctcaagg taggcggtgt ccgcggtgat gatatgctgc agcgcctctg
    15721 cgatagacca tgtattcgcg tcggctttcc aatggatctg cgcatccgaa aaagaccgga
    15781 tgtcgcggtc gagccgttct aatatcgccg tatagcgggc ctgcgtttgt tcaaaaaaat
    15841 cgttcataaa agattaatta tttcgtgtta cggttgatcg gcgcgaaaaa tatattacat
    15901 gttggcgaaa atcaatatgc aataaataga gatagttgtt tcgattgaat tatcaatcat
    15961 ggactcaatc gtataagtac gaactgcaac ttacgaagtg ttgatgtgtg agtcataagg
    16021 cgaaagaaaa tcatggaaaa tcactatggt ttttatgcca aattttgggt atgatgatat
    16081 gatctgtttt atatcaatca tctttattct ggggtaaaac aatgaaaaaa attctgaata
    16141 tctatttttt gtttggcgct ttgtacacct tggcgtatgc cggagatggt gtgacgaatg
    16201 agtttgtagc gacggcagga tggggcgctc cggtgggtat cggcttggag ttcgaaaaat
    16261 atattgttcc ggcattcaat ctcaatatgg gagccggtgt tacggtaacc ggggtgaagc
    16321 tgagtgtagg taccaagtac ttgatggccg ccgataaaga cgtatcgcca tacatcggtc
    16381 ttaatttctc gagttcttcg ggaatacgtc ggctaaacat aaatgttgat acggatacgg
    16441 ctaaatataa aataaatgcg gccaaaattt tgacaccacg ggcagggtta aagttaaaaa
    16501 gaggatttgc taatttttat ctcaacgtag gctatggtat cgtcgtggat ggcggaggta
    16561 ataagtacag gtcaggaagt acagataccg gcatatccga tatggcggat cttctgtccg
    16621 cgggcggcgt ggaaatcagc ggcagtattt gttttccgat caaatgacga attgacttgc
    16681 tgtccgacgt tgatcgcatg atgagctcat ttaatcatca gatgccaaaa tttttttgca
    16741 ctaagttgaa actctgtatg cgtgtaatgc tcactgtatt cgttatttta ttagttttat
    16801 cggatattct gtccgctcag gttagctact tgcagaacaa cgaatcgaat gccgttgcac
    16861 tcagtgccgg tgcgtccatc ggcagcaaaa acaacaagag tcgcgcgttg ggttttgaaa
    16921 ccggacaaag aaagtttgtg tttggagttt cctactctga aacaaagccc caatcagaag
    16981 acaaatacga tgccgtaagc gcacatatag gtacaacctt tccgcaccaa atcgatggga
    17041 tgaatctgag tctgcgattg ggatatttgg gtagctccaa gatcaagatt ccggccaaac
    17101 atgtagtgtt ttctccgtcg gtctttctca atattgcaca caaaattacc gcagattcgt
    17161 tcatcaaaat tatacctgaa gccggagttt cgcggttatt cgtttttgaa gaacaatatg
    17221 aaggcgatct ggtgagcagt gtaggtgtta ctattttgta taatagacct acgatggcgt
    17281 tcggattggg attgtcgagg aactggtcat ccggtcccgt cgtcaatctc ttatcattca
    17341 gtttgattac cgattgacgt agccgcttgg tctgccattt ttcagagata tagggtctcc
    17401 atttgatttc tccgattcag agcgtatatt acccccagtc ctcaaaaaat accacaaacc
    17461 ggagcacgtt ttatgaaccg tatccagctt gattacacgt atgtgatgcc gttcctgaag
    17521 gaagaagaga ttttcgggat gcaggccaaa gtcaacgacg tacataaaat gatcgagcaa
    17581 cgcaccggag cgggtaatga tttcttaggt tggctggatc tggcatccaa gtttccctcc
    17641 gcgcttgtag aggacatcgc taaaacggcg gaagatattc gtaaaacttc agatatcctt
    17701 atcaccatcg gcatcggcgg ttcatacctc ggcgcgaagg ccgttattga tgcgctgacg
    17761 catagtttta ataacaacat ccctcacaaa acgccgcagg tttattttgc cggccaaaat
    17821 atgagcggac gttatctacg cgaccttacc gaagtgatcc aaaacaaacg cgttgcggtc
    17881 aacgtgattt ccaaatccgg tacgacgacc gaaccggctc ttgccctacg gtattttcgt
    17941 aaaatcatga gcgatgccat gtccaaaatg caggtatcgc agcgaatttt tgcgacgacc
    18001 gatgccgccc gcgggtcact caaaaaatta gccgaaaaag aagggtacaa atgttatgtc
    18061 attcccgatg acgtcggcgg acgttattcg gtattgacgc cggtcgggct tttgccgatc
    18121 gctgtcgccg gcgtggatat ccgtgcgctt ttgcaagggg cggccgacat ggaaaaaata
    18181 ctgagcaacc cggatttaaa atccaatccg gcgtatgtct atgccgtgat tcgcaatctg
    18241 atgtacaaca aaggaaaagc catcgaaatt ctggccaatt tcgaaccgtc gctgcactat
    18301 gtgagtgagt ggtggaagca gctttacggc gaaagcgaag gtaaagatca caaaagtatt
    18361 tacccggcct cggttgattt ttcgacggat ctgcattcga tgggccaatg ggtgcaacaa
    18421 ggcacacgca atatctatga aacgtttttg tgggttgaca aagcacaatc ggatacctcg
    18481 gtgcccgacc tggaaggcga tgtggacgga tttaacttcc tcaccggtca gacgtttgaa
    18541 tacgtgaata aaaaagccta cgaaggcgtg acactggcgc atcgcgacgg cgaagtgccg
    18601 aatatgaccg tgcacttgcc ggaactcaac gcgtattatc tcggccagat gattttcttt
    18661 ttcgaaaaag cctgcgcgat gagcggttat ctgctgggcg tcaatccttt tgatcaaccg
    18721 ggcgtcgaag cgtataaaac caatatgttt gcattgctcg gcaaaaaagg gtatgaggaa
    18781 aaatcaaaag aactcaatgc cgtcttaggc caaatgcagc gtaaaaaaat ctgagtacga
    18841 tgatgacacg atcggaagcc ttacgcgcta ttctggaaaa agatcccggt gattcgtttt
    18901 cgcgttacgc gctggcgatg gagttagtcg gcgcacagcg atgggccgaa gcgatcgctg
    18961 agtttacgga tttacaacgg cgtgatccgt cctacgtcgc gctgtattat caactcggca
    19021 aatgttacga acaaaataat cagccgcagg acgccatagc cgcatatacg gagggcatcc
    19081 gcgttgcgcg acaagccaat aatatgcatg ccgtgtcgga attgtatgcg gccaaagaag
    19141 aactcgaaga tactttagct taaggaagcc atgccttttt ccaaaaaacc cggcgcccga
    19201 tctggcgcga aacgcacttc ggataaacgt acgtccgata aacgtacatc ggataagcgt
    19261 acctctgaaa atcgtacatc tgaaaaacgt acgtttgccg gtaaagaacg cggcgaaaga
    19321 tatagtgacg aacgtcctaa accacgccgg cgacctaaat actatcaacc gcgcacgttt
    19381 atgggtgcca attctgaaac ggaagaaagg cgtctcgccg agtggacggg ccagcggatc
    19441 gccctcgaag tattgcatgc attcgaagag acacaggagc gtgccgatat tctgcttcgc
    19501 acgcatattc agcgccatct cggcattttg gattcgcgcg atcgcgaaca gatcgcacgc
    19561 acggcgctca atgtgatccg gtataaatta cgtttggatt atgtggtttc caaaacgctg
    19621 aaacgcaatg cggaggaatt ggagccgctg attcgctgta tctaccgcct cgccgcgttt
    19681 catatcgtcc acctcggaca ctcggtcgaa cgtacgcagg cgattgtcaa ttttatagtt
    19741 cccaaatcgt acggcaacca aaaagaaaac ctccgtaatt ttatcagtta cctgaaaaaa
    19801 tatcacacgg aaatttcttt tcccaatccg cgcaaacaac ctgtagaagc gctggcgatc
    19861 acgtattcgc atccgtcatg gcttatcgaa aaatggagca agacgtacgg caccgagtcg
    19921 accgttcgga tgtgcgaatt tgataatcag gagacgcctt tggtcttgcg catcaatacc
    19981 cttaaggcga cggaagcgga tgtatttaaa aaattaaaag ccgaagggat tattcccaaa
    20041 aaaaccgcat acgcacaata cggacttatt cccggcgaag tgcatgatat ttttaatcgg
    20101 aaaatattta aagacggatg ggtcgaagtg caggccgaat ccagtcagat tttttgcgaa
    20161 tgggtcaatc cggcttccga ggatcgcgtg ctggatgcat gcgccggggg cggcggaaaa
    20221 tcgctggcgc tttcgatgct gatgcataac accggtcata tcgtcgcggc ggatatcaat
    20281 gcgggtgctt tggaacaatt aaaaaaacgc gcaacccgtg ccggctgtaa aaatatcgag
    20341 gtgatcgaaa acgatgcgtt ggcggcgcta aatcaacggg ggacatggta cgacaaaatc
    20401 atcattgatg cgccatgcag cggtctcggt gtattgcagc gcaatcccga tatgcgctgg
    20461 cgcgttacgc aggataatat cgaaaaactg gtcaacgaac agcggacgtt attggaaaat
    20521 tacgcgccgt tggtcaaacc cggcggcatg ctgatctatg caacatgtac attatccgaa
    20581 gaagaaaacg aaaacgtcat cgaagcgttt ttgtccgcgc atccggaatt tgaacttcag
    20641 aaaaatcatg atgcccgttg gaaaaaattt atttccggga aaggctattt ttgcatcacg
    20701 cctttcgaac ataatatatc cggatttttt tcgggcaaac tgatacggcg aaaaaactga
    20761 cagcttaaaa ggagcattat gaaaatatta gtattgggcg caggatggat gggacgtgcg
    20821 gtggtgtttg atcttttacg caatcaccac atggaacgca ttatcgtagc cgatgccaat
    20881 gcccaggcac tcaaagaaac cgaagcgttt gttaaagcgg aaaacgatct ccgtttttta
    20941 gccaaaaaaa tcaacgtcga agacgaagag gctttgaaaa aactgatgag tcaggttgat
    21001 accgtggtca gcgccgtgac gtataaatat aattacaagc tggcaaggct cgccgtccaa
    21061 tgcggatgca atttttgcga cctcggcggt aataatacca tcgttgcgca ggaactttca
    21121 ctgcatgata aagccaaaaa gaaaaatatt acgatcatac ccgactgcgg attggctccg
    21181 ggtatggttt ctgttttggt agcggatggc atcgggcaaa tggatgagac tacggatgtg
    21241 cagatacgcg tcggtggtct tccgctggaa ccgcgtcctc cgatgaatta taaactggtt
    21301 ttttcggtgc acgggttgat caacgaatac gtcgaacctt gcgtgaaaat ccaaagcgga
    21361 gaagttgtta ccgtggaccc aatggtggat gtggaggagc tggaatttcc cgcgccgttc
    21421 ggcaaactgg aagcgtttaa tacttcgggc ggcacctcga cactgccgca gacgtattta
    21481 ggaaaagtga aaaatttgga ttacaaaacg atacgatatc ccggtcacgc cgcacagttc
    21541 aagttgctga tggatctcgg actgacggaa tccaaaccgg tcaaagtcgg caacgatacg
    21601 gtcgtaccgc gcgatctgct cgcacaaaat ctaaccaaag t
//
LOCUS       DAOQGF010000052        21409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1133_length_21409_cov_7.596563, whole genome shotgun sequence.
ACCESSION   DAOQGF010000052 DAOQGF010000000
VERSION     DAOQGF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1133_length_21409_cov_7.596563"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..2166)
                     /gene="fusA"
                     /locus_tag="PK937_08685"
     CDS             complement(85..2166)
                     /gene="fusA"
                     /locus_tag="PK937_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNH32686.1"
                     /translation="MPRSISLEKTRNIGIMAHIDAGKTTTTERILYYTGVLHRIGEVH
                     DGAATMDWMEQEKERGITITSAATTCFWKNHRINIIDTPGHVDFTVEVERSLRVLDGA
                     IALFCAVGGVEPQSETVWRQADKYGVPRIAFVNKMDRIGADFFNVVKMMKERLGAHPV
                     PITFPMGEGDMFTGVIDLIDMKAIVFDESSQGMRWDVIEIPADLKEKADNARQFMLEQ
                     IADYDEVLMEKYLNGQDITKQEIQKAIHAGTLQCKITPVLCGSSFKNKGVQRLLDAVL
                     EYLPNPLEGGNLKGHNPDDHTQVIERRIDDSEKFCALAFKIMTDPYVGKLTFIRIYSG
                     MLKAGSYALNVTSGKKERIGRVLQMHANHREDIDACYAGDICAAVGLKFTKTGDTLSA
                     EDSPILLEKMEFPEPVIEVAIEPKTKADQEKLSEALSKLAEEDPTFRVSTNEETGQTI
                     IKGMGELHLEIIVDRMFREFKVEANVGKPQVAYKEGITKKVEAEGKFVRQSGGRGQYG
                     HVWIEVEPNEAGKGFEFTNGIVGGVVPKEYIKPTEEGIKEAMKNGVIAGFPLEDVKVK
                     LFDGSYHDVDSSEMAFKIAGSMAIQAAAKKAGPIILEPIMFVEVVVPEDYLGAVMGDL
                     NSRRGQIMGMNQRKDAQVVHAHVPLAEMFGYATALRSATQGRAIYSMHFHHYDPVPRA
                     VEAQLLEKVKA"
     gene            complement(2211..2681)
                     /gene="rpsG"
                     /locus_tag="PK937_08690"
     CDS             complement(2211..2681)
                     /gene="rpsG"
                     /locus_tag="PK937_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNH32687.1"
                     /translation="MSRRKRSPKRRIPADPKFNSVLVTKMINVIMWQGKRGVAESILY
                     DAMETVEKKTGKNGLEILEKAIKNVAPLLEVRSRRVGGANYQVPVEVRAERRQTLALR
                     WLIGYSRERAGKSMASKLADEILAASNNEGAAVKKREDVHKMAEANKAFAHFRW"
     gene            complement(2714..3103)
                     /gene="rpsL"
                     /locus_tag="PK937_08695"
     CDS             complement(2714..3103)
                     /gene="rpsL"
                     /locus_tag="PK937_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNH32688.1"
                     /translation="MPTIQQLIRKGRQSKTWKTKSPALQECPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLSNGIEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGVL
                     DTQGVQDRKQGRSKYGTKKGKEGAAKK"
     gene            complement(3516..3956)
                     /locus_tag="PK937_08700"
     CDS             complement(3516..3956)
                     /locus_tag="PK937_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH32689.1"
                     /translation="MEIKKLTADEVSDFKRLIEIFKDVFENDVDIPPVDHLRRLLSNP
                     DFMVFVALSGGNVAGGLTIYVLHRYYSVKPEAYIYDVGISPEFQRKGLGKALMAEVCR
                     YCQANGFENAYVEAEHDDVEAVRFYRATAYNHEVHAVHFTYVWR"
     gene            complement(3990..4658)
                     /locus_tag="PK937_08705"
     CDS             complement(3990..4658)
                     /locus_tag="PK937_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNH32690.1"
                     /translation="MYNFSYFKEKERQVIREFMEGNPFAFLTGSFLSGVQVGTQIPIL
                     FEERDGELYLQGHIMRHTDHHKALVENPNALVVFTGPSTYVSASWYSNPHIGSTWNYM
                     SVYISGKMKFMSSDELIRFMRKLTLKFEKGNTQSQTIYDNLPESFLNKMMPAIAGFEI
                     KADTLDNVFKLSQNRDEKSYMNIISKLEAQGGNSALIAQEMIKRKSELFPPGVAWDAS
                     KFDS"
     gene            complement(4828..5412)
                     /locus_tag="PK937_08710"
     CDS             complement(4828..5412)
                     /locus_tag="PK937_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH32691.1"
                     /translation="MTTDLKKFLSRYAGFSEAELENIAGKFKSQKLRKNSYMLRQGEV
                     CRNIVFVQKGCLRLFYRKDDVDVSVWFSFAQSSAIEIYSFISENPSDYYLQAIEESEV
                     LYLSKTELKKLYARHPNMQQMMRNFWEDVILNLIKRFTALQTDSAEKRYLDLLGKPDF
                     LGTIPQKYLASFIGVTPTSLSRIRRQITRKHKLS"
     gene            complement(5674..5847)
                     /locus_tag="PK937_08715"
     CDS             complement(5674..5847)
                     /locus_tag="PK937_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32692.1"
                     /translation="MKSDCCKNYKKKGKACRECPVLAKLRERHADLRRKEVFEYLQNL
                     KRNRFVCEVSYEP"
     gene            complement(5858..6190)
                     /locus_tag="PK937_08720"
     CDS             complement(5858..6190)
                     /locus_tag="PK937_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32693.1"
                     /translation="MADCFHRIVQQNEMGYVSVCEMCERIQVGFGTALANFTRREFSD
                     FRERIVREWMALKSEPNPREKNIFIETSSRAWHLALTGSELSKLTDLLEKADTVLSLT
                     SQRATYYN"
     gene            complement(6217..6339)
                     /locus_tag="PK937_08725"
     CDS             complement(6217..6339)
                     /locus_tag="PK937_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32694.1"
                     /translation="MNFKNRWPWAKKLGIIGFLFFLIKGVLWLIVPMATVWVCN"
     gene            complement(6336..6668)
                     /locus_tag="PK937_08730"
     CDS             complement(6336..6668)
                     /locus_tag="PK937_08730"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNH32695.1"
                     /translation="MSTHRFVAINYITCNDNYRERFEELFSSRAHAIDRMPGFVDMQV
                     LKPDQPDEPYLIVSHWDSKEAFDTWTQSPEFIEGHKRGFADVRAAKARGEVAPMESKF
                     KTYAVIAR"
     gene            complement(6668..6967)
                     /locus_tag="PK937_08735"
     CDS             complement(6668..6967)
                     /locus_tag="PK937_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH32696.1"
                     /translation="MKKYFFCLSLLLMSSCAPALYVPSAATTSDPAELTVLNEGRAMY
                     VQHCGSCHSLFVPSDFSDEAWEAHLDQMQTRAKISDHQKERILKYLTSYKKPEKK"
     gene            complement(6976..7767)
                     /locus_tag="PK937_08740"
     CDS             complement(6976..7767)
                     /locus_tag="PK937_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32697.1"
                     /translation="MKTFFLTLFLTWCGFNTLTAQDRIFNYTYQSNVLNAGQREVEPW
                     VTFKWGREKFYRAFENRIEYELGFAKNLQMAFYINAKTSAAQTNDSTIENATELSFSN
                     EWKYKVFDAVADPVGFALYGEYGIGRNEIEIEGKLIFDKRIGNSILALNLVGELEFEK
                     EAEHGEIENEKETVYELDLGYSYRVRNGLYLGIEARNHNEVVEGKWEHSALFMGPTLS
                     YTMENFWVNLTILPQVKALKGATKNGLVLDEHERLETRLIFSYIF"
     gene            complement(8337..9965)
                     /locus_tag="PK937_08745"
     CDS             complement(8337..9965)
                     /locus_tag="PK937_08745"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32698.1"
                     /translation="MLNNDGWTQQEGIEVLKTAIQGVAYDSGKVNLMHDLAFQYLEVD
                     LDSAEYYIRKSEELSRVFKYKFGEVKAVNYLGYVFEQRGRVDDAKRLYGDALKMAMNN
                     NDTVGLSRAYNSLAGIAMIGGKYREALGYYENMLGVYQGRADLQSQSVAYNNIGNVYV
                     EMGDHEQALEYYFKSLKIAQEAGDQKSLGTSYINLGNVFLEQSKYVEALEYYQKSLEI
                     QEKIGSQSGTALCFLNIGSVYEKQERLDSALHLYRKAYGLYKQLDNKSGVAWTLVSLG
                     DVYNRKGNYAEAATYLRQSLEMAKFVKDLTAEGNAYVSLAQNYKKLRMYYMALQYAGM
                     GHQIYLASGNRNRIRDVRLVFSEVYEAMGYLPQALTWYKGYIAIKDSLQSVGANRKTA
                     ELQLLYDSKRKDQQIALLQRDSTIKSLEISQSKIVRHSLIGGFLALIVVLVLLWQRYR
                     VKRQSEELLNNKNAELTKINSDLEEALHNVKELRGMFPICANCKKVRDDHGFWSQIET
                     YLQKHTDAEFTHSVCPECLNTLYPEFVKRAKEAE"
     gene            10372..12228
                     /locus_tag="PK937_08750"
     CDS             10372..12228
                     /locus_tag="PK937_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNH32699.1"
                     /translation="MMTWIREKTHWLLYALIVFFIALIVIEWGANYTDIANKSRGTIG
                     KINGEDIRYADFATVYNNQINNARAQKDGEALTDAEMEQLSDQIWNQIVEEKLLREYM
                     KENHVAVGDTEVVFELRNNPPEFIKNTPAFLTDGNFDMNKYQQALNNPQYAKEWAQVE
                     QMLRMQMPYNKLQAMIATSPRISESELRQEYARRNIKLNGQIVFFSPADIPASSVQVT
                     DAEAQSYYDNHKDDYKQEEKARLAFVSFSTEATKEDSAEVIERVNEVKKQVLEGKDFA
                     ELAKLNSVDRAANQQGGSLGWFTKGAMVREFEEACFNAKKGDVIGPVITSFGYHIIKI
                     DSTRFVKDKKARAKKAGEAEDRDSVLARHILIRMEASQTTIETARENANAFYETAKDN
                     GFDKALEKYASRFNLTSDTTLDIVNNENGIVAGFAERMKNVTRFAFTEKAGAVHRPHQ
                     TGRGVTIFAIVSRTDKGIQPFEVVKERVRNTVMEEKRMTMAYQKAQELRSKINSLADL
                     KALDSTLTVRDLTNFTMNNSIIGVGRDAKINGVLFNTTVGTLSEAIKGNRGAYIAFVA
                     TRDDINESLFRDARNDLRRQLQSTKQQRAYRDWVESMKKQATIKDFRPEFNL"
     gene            12321..12611
                     /locus_tag="PK937_08755"
     CDS             12321..12611
                     /locus_tag="PK937_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNH32700.1"
                     /translation="MKPITAIATQTEHEVSESTTLDHPLRVILFNDDIHTFDEVIAQV
                     IKAIGCTVQKAEAIAWEVHTRGKAVVYEGDLGECLRVSSVLEEIGLHTQIEP"
     gene            complement(12624..13613)
                     /locus_tag="PK937_08760"
     CDS             complement(12624..13613)
                     /locus_tag="PK937_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH32701.1"
                     /translation="MEKSIAPEHPIHVSSQTDIKTAALTVHDNKIAMRWSLFIGFGMF
                     AMKIYAYFITGSAAILSDAAESVVHVLAVSFAAYSLWLSTQPPDKNHPYGHDRISFFS
                     AGFEGAMIVMAALYIIYESVKKWIRGLEIHNIESGTMFVVIATAVNAALGWYLVHKGK
                     KNHSLILIANGKHVLTDSWTSFGVIFGLVLTMVTGWLPFDPIVAILVALNILWSGGKL
                     IRQAFGGLMDEADPETQTKLESILQAETQKYGIRFHDLKHRSSGNRIIIEFHLLFHDS
                     LTIVQAHAQATAIEKVIADTFPIETEITSHLEPIEGHNEAHITNTRMRNGKNE"
     gene            complement(13807..15276)
                     /gene="gltX"
                     /locus_tag="PK937_08765"
     CDS             complement(13807..15276)
                     /gene="gltX"
                     /locus_tag="PK937_08765"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNH32702.1"
                     /translation="MSVRVRYAPSPTGFLHIGGLRTALYNYLFARHHGGKFILRVEDT
                     DRTRLVEGAVDDFMNMLAWAGIQVDEGPGIGGSFGPYYQSERLHLYKQYAEQLLAEGK
                     AYRCFCTAERLDQMRKFQEKSKLPPKYDRTCLRLSAEEINKKIAAGEPHTIRMRVPDN
                     TVIRFKDHIRGDVEFTSDNVDDQVLMKSDGYPTYHLANVVDDHLMEITHVIRGEEWVS
                     STPKHVLLYQYFGWKAPEFAHLPLLLNSDRSKMSKRQGDTEARAYPPKGYLRDALVNF
                     IALLGWNPGDDREIFSMDELIKEFSIERIHKAGAVFDIQKLNWINTMYIRKLSDDEIY
                     AILKPDLEKAGYSDYPKQYVLRAIGLMKERVNFISEVLTFSSYFFRDPDTYDPEGVRK
                     RWTPQAKTYLTELAGRLENTASFIHADIEAVFRGYSEELKIKASELIHPVRIAISGMT
                     LGPGLFELLEVIGKEAVIRRMRRAIDVYDHVVGEAQSVK"
     gene            complement(15282..16529)
                     /gene="coaBC"
                     /locus_tag="PK937_08770"
     CDS             complement(15282..16529)
                     /gene="coaBC"
                     /locus_tag="PK937_08770"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNH32703.1"
                     /translation="MIQQTSGMMANDSEKYSGKKILIGITGGIAAYKIAELIRYFVKS
                     GAEVRVVMTKNATEFITPLTIETLSNNRVTTDTFPGHDAHYPEMWGTHHVNIAQWSDI
                     MLIAPAGGNVIGKIASGIADDALTTIVMACSKPVALAVAMNDRMYTNSIVQQNIKKLR
                     DHGYHIIDPGNGFLAEGYEGVGRLAEFESLIWEADKILLGTNELKNKRILITAGPTQE
                     PLDPVRILTNHSSGKMGYAIAREAALMGAEVTLISGPTALPAPPGVSVMQVTTADAMD
                     KAVRGQFTKHDALIMTAAVADFKPARQEKQKIKKTGAKNITIELARTPDILANIGKQK
                     GRRYVVGFALETDQEIENAQRKLHAKNLDFIVANNPKEQGAGFNTDTNIVTIIDKNSI
                     MRWPKMSKAQVARKILEKVAKEI"
     gene            complement(16606..16890)
                     /gene="rpoZ"
                     /locus_tag="PK937_08775"
     CDS             complement(16606..16890)
                     /gene="rpoZ"
                     /locus_tag="PK937_08775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNH32704.1"
                     /translation="MPVKAFPFDKIDASIENVYEAVIVAARRARQINDEQMIHVRTLT
                     EGDEAMDGEEVKINRDDILDLEKLPKPVATALDELLAGKIKHEYLNREEE"
     assembly_gap    17008..17107
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17108..19959)
                     /gene="gcvP"
                     /locus_tag="PK937_08780"
     CDS             complement(<17108..19959)
                     /gene="gcvP"
                     /locus_tag="PK937_08780"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNH32705.1"
                     /translation="MNTLHNDSFQKRHIGPRAEDLKSMLQAIGVGSLDELIEKTVPPS
                     IRLTSPMNLPPAIGEYEMLAELRALAAKNKIYKSYIGQGYYDTITPGVILRNILENPS
                     WYTQYTPYQAEIAQGRLEALLNYQTMVMDLTKMEIANASLLDEGTAAAEAMHMFHASA
                     NEGTGNKFFVASSCFAQTIDVMKTRANPLGIEIVVGDHTTFKPSKEYFGALIQYPAAD
                     GAIYDYAAFTQQCHEQGVYVAVAADILALTLLTPPGEWGADVVVGSTQRLGIPMGYGG
                     PHAAFFATKDEFKRKMPGRIIGVTIDANNKRALRMSLQTREQHIRREKATSNICTAQV
                     LLANMAGMYAVYHGPKGMREIGLRVHNRAVTLAGALVSAGYRQLNAVYFDTIKIDTGD
                     AKTYDAIRSAALAAQINLCYHGSQTITIAVDETTGAKDIEILTQIFAKAKSASVTVKH
                     SDLQTPSWGKPFVRQSAYLTHPVFNTYHSETEMLRYIKYLELKDVSLANSMIPLGSCT
                     MKLNATTEMEPVTWAEFGGLHPFAPSDQTAGYQQLFSELESYLNAITGFAGTSLQPNA
                     GAQGEYAGLLVIREYLKSIGQSHRHIALIPSSAHGTNPASAVMAGMQVVVVKCDDMGN
                     IDVKDLRTKSEQYKNELAALMVTYPSTHGVFEEAIHEICEIIHANGGQVYMDGANMNA
                     QVGLTSPAMIGADVCHLNLHKTFCIPHGGGGPGMGPICVAKHLVPFLPGHAVVKTGGN
                     KAMTAVSAAPWGSASILLISYVYIRLMGAAGLTDATKYAILNANYIRARLKDHYDVLY
                     VGKNGFVAHEMIMDTRGFKTTANIEVIDMAKRLMDYGFHAPTVSFPVAGTLMVEPTES
                     EPKSELDRFCEAMISIRNEIREIENGQAHKEDNVLKHAPHTAETVVSDTWTHAYTREK
                     AAFPLPTLRTNKIWPSVGRVNDAYGDRNLICSCPPV"
     gene            complement(19996..21033)
                     /locus_tag="PK937_08785"
     CDS             complement(19996..21033)
                     /locus_tag="PK937_08785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH32706.1"
                     /translation="MSRTYKILIVDDNPDHVYSLRKTLQRPEYVFFEAHSGEDALGSV
                     TAHDPDIMLLDVRLPGIDGFEICESIRKISAHLPIVFVTANLKEFVDQVHGFEKGGDD
                     YVIQPYDPRELAIKVKALLRNKVLYDSLLQEVNKLDRMKAELQQHNEELKKVNNKLEE
                     KTEHLKSLTVTDPLTALFNRKYFHQRINKEISAVKRYKAEASAAMVDIVNYTGINEQY
                     GVPQADVILKEMASLLVNSVRNSDIVTRFDGGKFAVIFTHTPEDSALIKARMIVEAVA
                     AYPFPIYDDLIPSEKINELDKKEIRIRAKISLTGLNHDWVRTEADLIEAFERSLLQNR
                     SNQDYVFFARK"
     gene            complement(21030..>21409)
                     /locus_tag="PK937_08790"
     CDS             complement(21030..>21409)
                     /locus_tag="PK937_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32707.1"
                     /translation="LIVDDDLSQAELIEEILESDDFLSEVATSGEKAIQAARRKSYDL
                     VILDLMMPQIDGFAVLSSLQSDSITQNLPVLVLTGKLLTQEDHRKLSGNHYYIFQKSA
                     FSREKLLEQIHRIMEAERNATKP"
BASE COUNT         5705 a         5049 c         4549 g         6006 t
ORIGIN      
        1 atcgtgccca cattcacgtg cggctttgaa cggttaaatt tctcttttgc catggttaag
       61 tctcctggtg gatatctatt tatattaagc ttttactttt tcaagcaact gcgcttcgac
      121 agcacgcggc accggatcat agtgatggaa gtgcatcgaa taaatcgcac gaccttgtgt
      181 cgcggaacgc aacgccgtag cataaccgaa catttcggcg agcggtacgt gcgcgtgcac
      241 gacttgtgca tctttacgtt gattcatacc catgatctga ccgcgacggg agttaagatc
      301 acccattacg gcgccgagat aatcttcggg tacaactact tccacaaaca tgatcggctc
      361 aaggatgatc gggccggctt ttttagcggc cgcctggatg gccatcgagc cggcgatttt
      421 aaacgccatt tcgctggaat ccacatcgtg atatgaaccg tcgaataatt taactttgac
      481 gtcttcgagc gggaagccgg caatcacacc gttcttcatc gcttctttga taccttcttc
      541 ggtaggcttg atgtattctt taggaaccac acctcccaca ataccgttgg taaattcaaa
      601 acctttacca gcttcattcg gttcgacttc gatccagacg tggccatact gaccgcgacc
      661 accggactga cgaacaaact taccttcggc ttcaactttt ttagtgatac cttctttata
      721 agctacttgc ggtttaccga cattcgcttc gaccttaaac tcgcggaaca tacgatccac
      781 gatgatttca aggtgcaatt cgcccatacc tttgatgatc gtctgacccg tttcttcgtt
      841 ggtcgaaaca cggaatgtcg gatcttcttc ggcaagtttg ctaagcgctt cggagagttt
      901 ttcctgatcg gctttggtct tgggttcgat cgcaacttcg atcaccggct cagggaattc
      961 cattttttct aaaagaatcg gagaatcttc cgcgctgagc gtatcgccgg tttttgtaaa
     1021 cttcaaaccg acagccgcgc agatatcgcc cgcatagcac gcgtcaatat cttcacgatg
     1081 gttggcgtgc atctgaagca cacgaccgat acgttctttt ttaccactgg taacgttgag
     1141 tgcatacgaa ccggctttga gcatacccga ataaatacgg atgaatgtca atttaccgac
     1201 ataaggatcc gtcataattt taaatgccaa agcgcaaaat ttttcactgt catcaatgcg
     1261 tcgttcaatc acttgcgtgt ggtcatccgg attatgacct ttgagattac cgccttcaag
     1321 cggattgggc aagtattcga gaacggcatc cagtaaacgc tgtacgcctt tgtttttgaa
     1381 agatgaaccg caaagtactg gagtgatttt gcattgcaaa gtaccggcgt gaatagcttt
     1441 ttgaatttct tgttttgtaa tatcctgacc attgagatat ttttccatca ggacttcatc
     1501 gtaatccgca atctgctcaa gcatgaactg acgcgcattg tccgcttttt ctttcagatc
     1561 ggcggggatt tcaatcacat cccagcgcat accttgggaa gactcgtcaa atacgatcgc
     1621 tttcatatcg atcaaatcaa tcacacccgt aaacatatcg ccttcgccca tcgggaaggt
     1681 gatcggcact ggatgcgcac cgagacgttc tttcatcatc tttacgacgt tgaagaagtc
     1741 tgcgccgata cgatccattt tattgacaaa cgcgatacgg ggaacgccgt atttatccgc
     1801 ctgacgccat accgtttccg attggggctc aacgccgccg actgcacaaa acaacgcaat
     1861 cgcaccatct aatacgcgca acgagcgctc tacttcgaca gtgaagtcaa cgtggccggg
     1921 cgtatctata atattaatac ggtgattttt ccagaagcat gtagttgccg cagacgtgat
     1981 cgtgatacca cgctcttttt cctgctccat ccagtccatg gtagccgcac catcgtgcac
     2041 ttcaccgata cggtgcagaa cgcccgtata ataaaggata cgctctgtcg tcgtggtctt
     2101 acccgcatca atgtgagcca tgatgccgat attgcgtgtt ttttctaacg aaattgatct
     2161 gggcatttta tttataagtt cctgttattc gtattttcta tttctgacta ttaccagcgg
     2221 aagtgcgcaa atgctttgtt cgcttcagcc atcttatgca catcttcacg ttttttgacg
     2281 gccgcgcctt cattattgga cgcggctaaa atttcatccg ccaatttaga agccatgctt
     2341 ttgccggcgc gttcacgaga gtagccgatc aaccaacgca aggccaacgt ttgacgacgt
     2401 tcggcacgca cttcgacagg cacttgatag ttggcgccac cgacgcgtcg ggaacgtact
     2461 tctaaaagcg gagcaacgtt tttgatcgct ttttccaaga tttccaaacc gtttttaccg
     2521 gtcttctttt cgacagtttc catcgcatca tacaggatgg attcggcaac gccgcgttta
     2581 ccttgccaca tgatcacatt gatcattttc gtgactaaga cgctgttgaa tttgggatcc
     2641 gccggaatgc ggcgcttggg acttcttttt cttctcgaca tatttcctct atatcaaact
     2701 tttttatcta agattacttc ttagcggcgc cttctttacc ttttttggtg ccgtatttgg
     2761 aacgaccttg tttacgatct tgaacacctt gcgtatccaa aacaccgcga acgatgtgat
     2821 aacgcacacc cgggagatct ttcacacgac caccacggat aagcacgatc gagtgctcct
     2881 ggaggttatg gccttcaccg ggaatatagg cgatcacttc gataccattg gagagacgca
     2941 ccttggctac cttacggagg gccgaattag gcttcttagg ggttgtcgtg tagacgcgcg
     3001 tacagacgcc gcggcgctgg gggcattcct gcaaagcggg cgacttcgtt ttccaagtct
     3061 tggattgtct gcctttgcga ataagctgtt ggatcgttgg cattaatgca cttcctgcta
     3121 aactgttatt tagtagttat actgtaacaa actctgtttt tactcttatt tgacagaaaa
     3181 cctgggggtt ttttatcatt gcctgcgcat ccgaactccg cttccgcgga aacctgaggc
     3241 aggttgagta tctaaaaagg cttgcaaata tatacgaaag agggtcgcat gtcaagagaa
     3301 aatatggaaa ttttaaaaat aattttcgtt gtgaaataaa gacttagctc tgtttcaaat
     3361 caatgacaat tcttcgatga gtgtttagcg ctgcatcaga gatacatttt ttaaaataat
     3421 aacagctgca gagctcaacc ttatatctca agtttatttc acataccgga tcgtaaaagc
     3481 ttgagaaaca aaacgctcat tattctaaca tcatattacc gccaaacata cgtgaaatga
     3541 acggcatgca cttcatgatt gtaagccgtg gctctgtaaa accggacagc ttcaacatca
     3601 tcatgttcag cttccacgta tgcgttttca aatccgttcg cttgacagta acggcatacc
     3661 tccgccatca aagctttccc taaacctttt ctttggaact ccggcgatat gcccacatca
     3721 tagatatacg cttccggttt cacactgtag tatcggtgca aaacatagat cgtcaggccg
     3781 cccgccacat tgccaccgct cagggcgaca aacaccataa agtccggatt ggaaagcaac
     3841 cgtcgcaaat gatccaccgg aggaatatcc acatcatttt caaacacatc tttgaagatt
     3901 tcaattaatc ttttgaagtc cgacacttca tcggcagtga gtttttttat ctccatcact
     3961 ttatggtgtt ttattgtttg ttagaaagat tacgaatcaa atttcgaggc atcccatgca
     4021 acgccgggag ggaataattc cgattttctt ttgatcattt cttgagcgat aagcgcacta
     4081 ttgccgcctt gcgcttcaag tttggaaatg atattcatat agcttttttc atcccggttt
     4141 tggctcagct taaacacgtt atccaacgta tccgctttga tctcaaatcc ggcaatagcc
     4201 ggcatcattt tattcaagaa ggattcgggc agattatcat aaatagtctg tgactgcgtg
     4261 ttgccttttt caaatttcag tgtcaacttt cgcatgaacc ggatcaactc atccgacgac
     4321 atgaacttca tctttccact gatatagacg ctcatataat tccatgtcga accgatgtga
     4381 ggattactat accacgaggc gctcacgtac gtactgggcc ccgtaaaaac aaccaaggca
     4441 ttcggatttt cgaccaaagc cttgtggtgg tccgtatgtc gcatgatgtg cccctgcaaa
     4501 tacaactctc cgtcgcgctc ttcgaacaaa atcggaatct gcgttcccac ttgtactccg
     4561 gacaaaaaac tacctgtcaa aaaagcaaac ggattacctt ccataaactc ccgtattact
     4621 tgccgttctt tttctttgaa atacgaaaag ttatacatac gaatctcctt cggttatagg
     4681 tttaaaatac ctgttttgtc ctatcgcatg ggatgcacct tgagcggcat caaaaaaatt
     4741 ttatgcccgt atgtctgaaa cgacataatc ctgtccgctc aaaatgacaa tattaaacta
     4801 caaccttttt gatctaaaaa agttgtctta ggacaattta tgttttcggg taatttgccg
     4861 tcgtatacgg cttagcgagg tgggggtaac accgatgaat gaagcgaggt atttttgcgg
     4921 gatcgttcct aaaaagtccg gtttgccaag caggtccaga tatctttttt cggccgaatc
     4981 ggtctgtagc gccgtaaatc gtttgatcag gttaagaatg acatcttccc aaaagttacg
     5041 catcatctgt tgcatgttcg gatgacgcgc gtagagtttt ttgagttcgg tcttggaaag
     5101 gtagagtact tcactctcct cgatggcttg caagtaatag tcggaaggat tttcactgat
     5161 aaaactgtat atctcgatcg cggaggattg cgcaaaagaa aaccatacgg atacatctac
     5221 gtcgtctttt cgataaaata aacgcaaaca gcctttttga acaaacacga tatttctgca
     5281 cacttcacct tgccgcagca tataactatt ctttcgaagc ttctggcttt tgaactttcc
     5341 ggcaatgttt tccaattccg cttccgagaa gcccgcatac cggctgagaa atttttttag
     5401 atcggtggtc atgttaagaa ttgttacagt gtaagtaaaa cacgaaaaag ataaataata
     5461 atatgttttt ataaaaaaaa cacttatgaa aatcttcaga tttaaaaaaa taaaaacagt
     5521 acgtgatcac caaggtgaaa tgataagcac caataacttt taaaagtatg cttcaggatg
     5581 tcaaccggag ggtaaatatt ttttcggaaa ttgtgtgagg gataagacgg aaactaaatg
     5641 cggtgtgttt tgacttttat atactatcag gaattaaggt tcatacgata cttcgcatac
     5701 gaaacggttt cttttgagat tttgaaggta ttcgaatact tctttgcgac gcagatcagc
     5761 atgacgctcc cgcagcttgg ccagtacggg acattcgcgg caagccttcc cttttttctt
     5821 atagtttttg caacaatcgg atttcatcgt tgatccctca gttataatac gtcgcgcgtt
     5881 gacttgtcaa actcagtacc gtatccgctt tttccaataa atccgtcagt ttggaaagct
     5941 ccgaacccgt gagcgccaaa tgccatgctc tggaagaagt ttcgatgaaa atattttttt
     6001 cgcgcggatt aggttccgat ttcagagcca tccactcacg aacaatacgt tctctgaaat
     6061 cggaaaattc ccgccgtgta aaattagcta gcgccgtacc aaaaccaacc tgtatgcgtt
     6121 cgcacatttc gcagacggag acgtagccca tctcgttttg ttggacgatg cgatgaaaac
     6181 aatcagccat tcggtaaatc cgtcattttt gtgatactag ttgcacaccc atactgtggc
     6241 catgggcaca atgagccaca atacgccttt gattaaaaaa aacaaaaaac cgatgatacc
     6301 gagttttttg gcccacggcc agcgattctt aaaattcatc gcgcgatcac cgcataggtt
     6361 ttaaatttgg actccatagg tgcgacttct ccgcgcgctt tggcagcacg tacatcggca
     6421 aatccgcgct tgtggccttc gatgaattcc ggcgattggg tccaggtatc aaacgcttcc
     6481 ttgctatccc aatgactcac gatcagatac ggctcgtccg gttgatcggg tttgagaacc
     6541 tgcatatcca caaatcccgg catgcggtca atggcatgcg cgcgtgaact gaaaagttct
     6601 tcaaatcgtt cacgataatt atcgttgcaa gtgatataat tgatcgccac aaaacgatgc
     6661 gtactcatta ttttttctcc ggttttttat agctggtaag atactttaaa atccgttctt
     6721 tttgatgatc ggaaatttta gcccgcgtct gcatttgatc taaatgcgcc tcccatgctt
     6781 catcggaaaa atccgacggt acaaaaaggc tgtggcaact tccgcaatgc tgcacataca
     6841 tcgcgcgccc ttcgtttaaa accgtaagtt cggcgggatc actcgttgta gcggcgctcg
     6901 gtacatagag cgccggtgca caactagaca taagcagcag cgaaaggcaa aaaaagtatt
     6961 ttttcatggc tgtgctcaga atatgtaaga gaaaatcagg cgcgtttcga ggcgctcatg
     7021 ttcatccaat accaaaccgt ttttggtggc gccttttagc gctttaacct gcggcaagat
     7081 ggtcagattg acccaaaaat tttccattgt gtagctcagc gtgggaccca taaataacgc
     7141 ggaatgttcc catttgcctt cgaccacttc attatgattg cgggcttcaa tacccaagta
     7201 taatccgttg cgaacgcggt aactatatcc gagatccaat tcgtaaacag tttctttttc
     7261 attttcgatc tcaccgtgtt ctgcttcttt ttcaaattcc agttcaccga caagattaag
     7321 cgccagaatg gaattcccga tacgtttatc aaagatcaac ttgccttcga tttcaatctc
     7381 attgcgcccg ataccgtatt cgccatacaa tgcaaatcct acgggatcgg ccacggcgtc
     7441 aaatactttg tatttccatt cattggaaaa actcagttcg gttgcatttt ctattgtcga
     7501 gtcattggtc tgagccgccg acgtttttgc gttgatataa aatgccattt gaagattttt
     7561 ggcgaagccg agttcatatt cgatgcgatt ttcaaatgct ctgtaaaatt tttcgcggcc
     7621 ccatttgaag gttacccaag gttcgacttc acgttggccg gcattgagta cattgctttg
     7681 gtacgtgtag ttgaaaatac gatcttgagc ggttaatgta ttaaatccac accatgtcaa
     7741 aaacagggtc aaaaaaaacg ttttcataaa acaatcctcg gttaatattt aaagagttat
     7801 aattgaaatt gagattcagt ctcaatatta aaaataaata aaacaaattt tattttccta
     7861 ttatctaata actgagaacg gttctcaatg ataattatca tgtttttatg taattataat
     7921 ataaaattgt ttattattaa ttgaaattga gacttagtat caattgatga ttaatatata
     7981 ttcattattg attattttgt caagaattat ttttcgtaac tgataattac gaatttactg
     8041 acaacctcat cgtctatagc ttttccaaaa tctaaggttt tagttgatac ttttctattc
     8101 ccttattcaa aaggacttga attaaacaag attgcgttgt cttactttta aataaaaagc
     8161 aaactaaaca ccgtatttat taactaccaa acacgcgata catcagaaga agaaataaac
     8221 tttgcgcata aacgccgatc ttaaagcatt atgaaaacaa atcagggata gataaatcag
     8281 gcaggatatt caaaagaaaa aggagaaatt cattccaaat cgaataacga tcgacactat
     8341 tccgcttctt tggcgcgttt gacgaattca ggatagagtg tattaaggca ttcggggcag
     8401 acactgtggg taaactccgc atcggtatgc ttctggagat acgtctcgat ttgactccaa
     8461 aatccgtggt cgtcgcgtac ttttttacaa ttggcgcaga tcggaaacat gccgcgtaac
     8521 tctttgacat tgtggagcgc ttcctccaga tcgctgttta ttttagtcaa ttcggcattt
     8581 ttgttattca gtaattcttc cgattgacgt ttgacacgat agcgttgcca cagtaacacc
     8641 aaaaccacga tcaacgccaa aaatcctccg atcagtgaat ggcgcacaat tttactttga
     8701 ctgatttcaa gcgatttgat cgtactgtcg cgctgaagaa gagcgatctg ctgatctttg
     8761 cgttttgaat catagagcaa ctgaagttcc gctgttttac ggtttgctcc tacgctctgt
     8821 aacgaatctt tgattgcgat ataacccttg taccaagtca atgcctgcgg caaatacccc
     8881 atcgcttcat agacttctga aaacacaagg cgcacatcac gaatgcgatt gcgattaccc
     8941 gatgcaaggt aaatctgatg ccccattcct gcgtattgca aggccatata gtacattctg
     9001 agctttttat aattttgagc caagctgaca tacgcattac cttcggcggt aagatctttc
     9061 acaaacttcg ccatttcgag tgattggcgc aggtatgtcg ccgcctccgc ataatttccc
     9121 tttcggttgt atacgtcgcc aaggcttacc aatgtccatg ccacgccgga tttattgtca
     9181 agttgcttat acaacccgta tgctttccga tataaatgta gagcgctatc tagacgttct
     9241 tgcttttcgt agacagaacc gatatttaga aaacaaagcg ccgtaccgga ctgggaacct
     9301 attttttcct gtatttccag cgacttctga taatactcta acgcttcgac atatttggat
     9361 tgttccagga aaacattacc gagattgata tacgatgtgc ctaggctttt ttggtcgccg
     9421 gcttcttggg caattttgag cgatttgaaa taatattcca aagcctgctc atgatcaccc
     9481 atttctacat acacgttgcc gatattattg tatgcgacag actgactttg taaatccgct
     9541 cttccctgat agacgcccaa catgttttcg taataaccga gcgcttcacg gtatttaccg
     9601 ccgatcatgg caatgccggc aagactattg taagctcgcg acaaaccgac cgtatcatta
     9661 ttattcatcg ccattttcaa cgcatcgcca tagagacgct tagcatcatc tacacggcct
     9721 cgctgctcaa aaacgtaacc gagataattg acggctttga cttcaccaaa tttgtattta
     9781 aaaacgcggc tgagctcttc ggatttgcgg atataatatt cggccgagtc gagatctact
     9841 tccagatatt ggaatgccag atcgtgcatc agattgactt taccggaatc atacgccacc
     9901 ccctgaatgg cggtttttaa aacttcaatg ccttcttgct gagtccatcc gtcgttatta
     9961 agcataacgg cgcaaaaaac cgatgacaaa ataatccaac ggcggatatg tcgaaaccaa
    10021 tgattacgga aatgaaacaa gtagcgcatg tgtaatggat gtatgttgag aaaaccaaaa
    10081 gatatgcctt aaacactcta aaataccgct ataagacctc agaatcaaca gcttattggg
    10141 cgattgcaca atacaagtag ttctacgtac tcatttccta accctaaacg gagagatatt
    10201 ttcatatttc tccgtgtttt tttcccggtt tttcatcggc tttataaaac aactctaaac
    10261 aggaaaaaga attgcattta ttgaggtttt ggcctatctt gcccagcttt aatatgaagc
    10321 cgtaaataat tatcaaattg actataaagt tattgcagga gaaatgctcg tatgatgacg
    10381 tggattcgtg aaaaaacgca ctggttactc tatgcgttga tcgtattttt tatcgctctg
    10441 atcgtgatcg aatggggcgc taactacact gacatcgcca ataaatcccg cggcacgatc
    10501 ggcaaaataa acggtgaaga tattcgctac gccgactttg ctaccgtgta taacaaccag
    10561 atcaataatg cccgcgccca aaaagacggc gaagcgttga ccgacgcgga gatggagcaa
    10621 ctcagcgatc agatttggaa ccaaatcgtc gaagaaaaat tgctgcgcga atacatgaaa
    10681 gaaaaccatg ttgctgtcgg cgataccgaa gtggtatttg aactgcgcaa caatcctccg
    10741 gagtttatca aaaacacgcc tgcgtttttg accgacggta attttgatat gaataaatac
    10801 cagcaagcgc tcaataatcc tcagtacgcc aaagaatggg cgcaggtcga acaaatgctg
    10861 cgtatgcaaa tgccgtacaa taagcttcag gctatgattg ctacctcacc tcgtatcagc
    10921 gaaagcgaat tgcgtcaaga gtatgcccgt cgcaatatca agctgaacgg ccagattgta
    10981 ttttttagcc ccgcagatat tcccgcatcc agcgtacaag tgaccgatgc ggaagcgcag
    11041 tcgtattacg acaaccacaa agatgattac aaacaagaag aaaaagcgcg actcgctttt
    11101 gtttcgttca gtacggaagc taccaaagag gattccgctg aagtcatcga acgtgtcaat
    11161 gaagtaaaaa aacaagttct cgaaggtaaa gattttgccg aattagccaa actcaactcg
    11221 gttgatcgtg cggccaatca acaaggcggt tcgctcggtt ggtttaccaa aggagcgatg
    11281 gttcgtgaat ttgaagaagc ctgtttcaac gccaaaaaag gtgatgtgat cggccctgta
    11341 attacgtctt tcggttatca cattattaaa attgactcca cgcgttttgt caaagataaa
    11401 aaagcacgtg cgaaaaaagc cggcgaagcc gaagaccgtg atagtgtctt ggcgcgccat
    11461 attcttattc gtatggaagc cagccagacg acgatcgaga cagcccgtga gaatgccaac
    11521 gcattttatg aaacagccaa agataacgga tttgacaaag cgctcgaaaa atacgcatca
    11581 cgctttaacc taacgtccga cacgacgctg gacatcgtca ataatgaaaa cggtatcgtt
    11641 gccggttttg ccgaacggat gaaaaacgtg acacgttttg cctttaccga aaaagccggt
    11701 gcggtccatc gcccgcatca aaccggacgc ggcgttacaa tttttgcaat cgtttcacgc
    11761 accgataaag gtattcaacc tttcgaagtc gtcaaagagc gcgttcgcaa tacggtgatg
    11821 gaggaaaaac gcatgacgat ggcctatcaa aaagctcagg agttacgttc caaaatcaac
    11881 tcgctggctg acttgaaggc gcttgattct acattgaccg tacgcgatct gactaatttt
    11941 acgatgaata actccatcat cggtgtcggt cgtgacgcta aaatcaacgg cgtattattt
    12001 aataccaccg tgggtacgct gagtgaagcc atcaaaggca atcgtggcgc ttatattgca
    12061 tttgtcgcta cacgtgatga tatcaatgaa tcgctcttcc gcgatgcacg gaatgatctg
    12121 cgtcgccaat tgcaaagcac aaaacaacaa cgcgcttatc gcgactgggt cgaaagcatg
    12181 aaaaagcaag cgaccatcaa agatttcaga ccggaattta atttgtaagc gcttatttaa
    12241 aaatcgttct tcaagaaatc cggatgatga atccggattt cttattggat gatgattagt
    12301 tttttaatag attcctcatt atgaaaccca ttacggcaat cgccacccaa acggaacatg
    12361 aagtttccga atcaactacc ttagatcatc ctttacgtgt catccttttc aatgatgaca
    12421 tccatacctt tgatgaagtg atcgctcagg tgatcaaagc catcgggtgt accgttcaaa
    12481 aagccgaagc tatcgcatgg gaagtgcata cccgaggaaa agccgtcgtc tatgaaggtg
    12541 atttagggga atgtttgcgg gtcagcagcg tacttgagga aatcggctta cacactcaga
    12601 tcgaaccctg atcggttctc tccttattcg tttttaccat ttcgcattct tgtatttgtg
    12661 atatgcgctt cgttgtgtcc ttcgataggt tccaggtgcg atgtgatttc tgtttctatc
    12721 ggaaatgtat cggcgataac tttttcgata gctgtggctt gggcatgtgc ctgcacgatc
    12781 gtcaagctgt catgaaacaa aagatgaaac tcaatgatga tccgatttcc tgaactgcga
    12841 tgttttaaat catgaaaacg aattccgtat ttttgtgttt ccgcttgcag gatagattct
    12901 aatttggttt gcgtctccgg atccgcttca tccatcaatc cgccaaacgc ctgacgtatc
    12961 aatttgcctc ccgaccataa aatattgagc gcaactaaaa tagccacgat cggatcaaac
    13021 ggtaaccatc ccgtcaccat cgtcaacact aatccgaaaa tcaccccgaa actggtccaa
    13081 ctgtcggtca gaacgtgttt accattggct attaaaatca gcgagtgatt cttcttccct
    13141 ttgtgaacga gataccaacc caaggccgcg ttgacggctg tggctatcac gacgaacatc
    13201 gtacccgact caatattatg aatttccaat ccgcgaatcc acttcttcac cgattcatag
    13261 atgatataaa gcgccgccat cacgatcatt gcaccttcaa agccggccga aaaaaaactg
    13321 atacgatcgt gcccgtaggg atgattttta tcgggtggtt gcgtactcaa ccagaggcta
    13381 tacgcggcaa aggataccgc cagaacgtga actaccgact ctgccgcatc cgaaagaatc
    13441 gcagcggaac cggtaataaa gtaagcgtat atcttcatcg caaacattcc aaatccgata
    13501 aacaacgacc agcgcatggc gattttattg tcatgcacgg tgagtgcggc agttttgatg
    13561 tccgtttgtg aagaaacgtg aatcggatgt tcgggtgcta ttgatttttc cataattttt
    13621 taggaattaa aaaccgcatc aatgtacgta tctgaaaagc taaacgataa cattttttcg
    13681 cttagcatga gacaaggtct catgcgtgtg cattaaatca aataacccga cctatgggcc
    13741 gggctactga aataaaaaaa ttgttaaaca aaatgcggtt ttacattaac gaatcataac
    13801 catttcttat tttaccgatt gcgcttcacc gacgacgtga tcatacacgt caatagcgcg
    13861 acgcatacga cgtatgacgg cttccttacc gatcacttca agcagttcaa ataatcccgg
    13921 tccaagagtc attccggaaa tcgcaatgcg taccggatga ataagttcac tggctttgat
    13981 tttgagttct tccgaataac cgcgaaatac ggcttcgatg tccgcgtgaa tgaatgacgc
    14041 cgtattttct aaacggccgg ccaattccgt caaataggtt ttagcctgtg gtgtccagcg
    14101 tttgcgcacg ccttccggat cataggtatc gggatcgcgg aaaaaatagc tcgaaaacgt
    14161 cagcacttcg gatataaaat tgacgcgttc tttcatcaag cctatcgcac gcaaaacata
    14221 ttgtttgggg taatcggaat aacctgcttt ttccaaatcc ggtttaagga tcgcatatat
    14281 ttcatcatcc gaaagttttc gaatatacat cgtgttaatc caattcaact tttgaatatc
    14341 aaacacggca ccggctttgt gaatacgttc gatcgaaaat tctttgatca gttcgtccat
    14401 cgaaaaaatt tcgcgatcat caccgggatt ccaacccaac aaagcaataa aattgaccaa
    14461 cgcatcgcgc agataaccct tgggcggata cgcgcgcgcc tcggtatcac cttggcgttt
    14521 gctcattttg ctgcgatcgg agttgagaag gagcggaaga tgggcaaact ccggcgcttt
    14581 ccagccaaaa tactgatata gcagaacgtg cttcggcgtg ctggaaaccc attcttctcc
    14641 gcgaatcaca tgggtgattt ccataagatg atcgtccacg acattggcaa gatggtacgt
    14701 cggatacccg tcggatttca tcaatacctg atcgtccacg ttgtcggaag taaactccac
    14761 atcaccgcga atatgatctt taaaacgaat taccgtatta tccggcacac gcatacgaat
    14821 agtgtgtggt tcaccggcgg cgattttttt attaatttcc tcagcggata gacgcaaaca
    14881 tgtgcggtca tatttaggag gcaatttgga tttttcttga aatttacgca tttgatccag
    14941 gcgttccgcc gtgcaaaaac agcgataggc tttgccttca gcgagcaatt gctccgcata
    15001 ctgtttatat aaatgaaggc gttcggattg ataataagga ccaaatgaac cgccgatgcc
    15061 cggtccttcg tcaacttgta tgccggccca cgccagcata ttcataaaat catccactgc
    15121 gccttcgacc aggcgcgtac gatccgtatc ttcgacacgt aagataaatt ttccgccatg
    15181 atgacgtgca aataaataat tatataaggc tgtgcgaaga ccgccgatgt gaagaaaacc
    15241 cgtagggctt ggggcataac gaacacgaac tgacatactt tttatatctc ctttgcaact
    15301 ttttcgagaa tcttacgagc aacttgcgct ttgctcattt tgggccagcg cataatacta
    15361 tttttatcta tgattgttac gatattggtg tcggtattaa aacctgcgcc ttgttctttg
    15421 gggttattgg ctacaataaa atccaggttt tttgcgtgca atttacgctg cgcattttct
    15481 atttcctgat cggtttcgag agcaaatccc acgacatatc ggcgaccttt ttgtttgccg
    15541 atattcgcca aaatatccgg cgtgcgtgcc aattctatcg taatattttt ggcgccggtt
    15601 tttttgattt tttgtttttc ctgtcgtgcc ggtttaaaat cggcgaccgc agcggtcata
    15661 atcaacgcat catgtttggt aaactgacca cgcacggctt tatccatagc atcggctgtc
    15721 gtaacttgca tcaccgacac acccggcggc gccggaagtg cggttggacc tgaaatcaac
    15781 gtcacttcag cgcccatcaa cgccgcttca cgagcgatgg cgtaacccat tttacctgag
    15841 gaatgattgg tcaaaatacg cacgggatca agcggctctt gggtcggtcc ggcggtgatc
    15901 aaaatgcgtt tgttcttcag ttcatttgtg cccagcaaaa tcttatccgc ttcccagatc
    15961 aacgattcaa actcggcaag acgccccaca ccttcataac cttcagctaa aaagccgtta
    16021 cccggatcaa taatatgata tccgtggtca cgcaattttt taatgttttg ttgaacaatg
    16081 gaattggtgt acatacgatc attcatcgca acagccaatg ctacgggttt ggaacaagcc
    16141 atgacgatag tcgtcagtgc atcatcggca atacctgaag cgattttacc gatcacatta
    16201 ccgccggccg gtgcgatcag catgatatca gaccactggg cgatattgac atgatgcgta
    16261 ccccacattt caggataatg cgcatcgtgt cccggaaacg tatcggttgt aacgcggtta
    16321 ttggaaagcg tttcgatcgt cagcggcgta ataaattccg tggcattttt ggtcatgacc
    16381 acgcgcactt ccgcacccga tttgacgaaa taacggatca gctcggcgat tttatatgcc
    16441 gcgataccgc cggtaatacc gatcagtatt ttctttccgg aatatttttc agaatcgttt
    16501 gccatcatgc cagaggtttg ctgtatcatc cataaacggc aaattccaaa taaccggcgt
    16561 tataccggca tttggaattt gcatcgaaac ttttgtaaaa cgcggttatt cttcttcgcg
    16621 attgagatat tcgtgcttga ttttaccagc gagcaattca tccaacgccg ttgcgacagg
    16681 tttgggaagt ttttccaaat ctaaaatatc atcgcggttg atttttactt cttcaccatc
    16741 cattgcttca tcgccttctg tcaacgtgcg cacatgtatc atttgttcgt cattgatctg
    16801 acgtgcacga cgcgccgcaa cgatcacggc ttcgtagaca ttttcgatcg atgcatcaat
    16861 tttatcaaag ggaaacgctt tgaccggcat cgtgaactcc ttattgaaat tattacactt
    16921 atattgccaa atgggacgcc aatatagacg ttttgtccta ttcgcgcaat agaaaaatat
    16981 atattcatca ttatttctaa taataacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnntct accggcgggc aactgcaaat cagattgcgg tcgccgtacg catcattgac
    17161 gcggccgacg gaaggccata ttttattggt acgcaacgtc ggcaatggga acgctgcttt
    17221 ttctcttgta taagcgtgcg tccatgtatc ggaaaccacc gtttcagccg tatgcggtgc
    17281 atgtttgagc acgttatctt ctttgtgcgc ttgaccattt tcgatttctc gaatttcatt
    17341 gcgtatggat atcatcgctt cacaaaaacg atccagctcg gatttgggtt cggactctgt
    17401 cggttccacc atcagcgtac cggccaccgg aaacgaaacg gtcggcgcat gaaatccgta
    17461 atccatcaga cgtttggcca tatcaattac ttcgatattg gcggttgttt taaacccgcg
    17521 tgtatccatg atcatctcgt gcgctacaaa accatttttc ccgacgtaga gcacatcgta
    17581 gtgatctttg agacgtgcac gaatatagtt ggcgttgaga atggcatatt tggtcgcatc
    17641 ggtcaatccc gccgcgccca tcagacggat gtacacgtat gatatgagga gaatactggc
    17701 gctgccccat ggcgctgccg aaaccgccgt catcgcttta ttaccgcctg ttttgactac
    17761 cgcatgaccg ggtaaaaatg gaacaagatg tttagccaca cagatcggac ccatgccggg
    17821 gccaccgcct ccgtgcggaa tacaaaacgt tttatgtaaa ttgagatggc acacatccgc
    17881 gccgatcatc gccggactgg taaggccgac ttgtgcattc atatttgcac catccatata
    17941 aacctggccg ccattggcat gaatgatctc gcatatttca tgaatcgctt cttcaaatac
    18001 accgtgcgtt gaaggataag tcaccatcag agccgcaagt tcatttttgt attgctcgga
    18061 tttagtgcgc aaatctttta catctatatt tcccatatca tcgcatttta cgacgacgac
    18121 ttgcattccg gccatcacag cgctcgcggg gttcgtgccg tgtgccgagg acgggatcaa
    18181 tgcgatatga cgatgcgact gacctatgct tttcaggtat tcacggatca caagtaaacc
    18241 ggcatactcg ccctgcgcac ccgcgttggg ttgcagcgaa gtgccggcaa atccggtgat
    18301 cgcattaaga tacgactcca gttcgctaaa aagctgttga tagccggccg tttgatccga
    18361 cggagcaaac ggatgcaggc cgccaaactc ggcccaggtt accggttcca tctcggtggt
    18421 cgcattcagt ttcatagtac acgaaccgag agggatcatc gaattagcca acgacacatc
    18481 tttcaattcc aaatatttga tgtagcgcag catttcggtt tcactgtgat acgtattgaa
    18541 taccggatgc gtaaggtaag cgctttgacg aacaaatggt ttaccccacg acggcgtttg
    18601 taaatccgaa tgttttacag tgaccgaagc ggatttcgct ttagcaaaaa tttgtgtcag
    18661 aatttctata tcttttgcac cggtcgtctc atccacggcg atcgtgattg tttgcgatcc
    18721 gtggtaacac agattgattt gtgctgccag tgccgcgctt cgaatcgcat catacgtttt
    18781 ggcatcgccg gtatcaattt tgatcgtatc aaaatacacc gcgttcaact gacggtaacc
    18841 ggcggacacg agtgcaccgg caagcgttac ggcacgattg tgaacgcgca agccgatttc
    18901 cctcatccct ttgggaccgt ggtaaaccgc gtacataccg gccatattag ccagcaacac
    18961 ttgcgctgta caaatattgc tcgtcgcttt ttcacggcga atgtgctgct ctcgtgtttg
    19021 caatgacata cgtaacgcac gtttgttatt cgcatcaatg gtcactccga taatgcgacc
    19081 cggcatctta cgcttaaatt catctttcgt cgcaaaaaaa gccgcgtgcg gtccgccgta
    19141 acccatagga atacccaagc gctgcgtgga accgacaacg acatccgcac cccattcacc
    19201 gggcggtgtt aacaacgtaa gcgccaatat gtccgccgct acggcgacgt aaacaccttg
    19261 ttcgtgacat tgctgtgtaa atgcggcata atcatagatc gcgccatccg ctgccgggta
    19321 ttggatcaac gcgccaaaat attctttcga aggtttaaac gtcgtatgat cgccgaccac
    19381 gatttcgatg cccaaaggat tggcgcgtgt tttcatcaca tctatcgttt gcgcaaaaca
    19441 cgacgaggca acaaaaaatt tattgccggt accttcattg gcggaagcat gaaacatgtg
    19501 catggcctcc gccgccgccg taccctcatc caagagcgac gcgttggcta tttccatttt
    19561 ggtgaggtcc atgaccatgg tttgataatt cagcaatgct tccagacggc cttgcgcgat
    19621 ttcagcctga tacggcgtgt actgtgtgta ccaactcggg ttttccagta tgttccgcag
    19681 aattacgccg ggcgtgatcg tatcataata gccttgcccg atgtacgact tgtaaatttt
    19741 attttttgcc gctaaagcac gcaattcagc gagcatctca tattcaccga tggccggagg
    19801 caaattcatc ggcgaagtga gacgaatgga agggggaacg gttttttcga tcagttcatc
    19861 caaggagccc acaccgatgg cctggagcat ggatttcaaa tcctctgcgc gtggtccgat
    19921 atgacgcttt tggaagctgt cgttatgcaa cgtattcata ttaactcctt atgggaaaat
    19981 tcgatataag cgggattact tgcgggcaaa aaatacatag tcttgatttg agcggttttg
    20041 caataaggat cgttcaaaag cttcgatgag atcggcttcc gtgcgcaccc agtcatgatt
    20101 taatccggtc aacgatattt ttgcacggat tcggatttct tttttatcca gttcatttat
    20161 tttttcagac ggaataagat catcgtaaat gggaaaaggg tatgcggcta cggcttccac
    20221 gatcatacgc gctttgatca acgcactgtc ttcgggcgtg tgcgtaaaaa taacggcaaa
    20281 tttaccgcca tcaaaacgcg tcacaatatc ggaattacgg acgctattga ccaacaggct
    20341 ggccatttcc ttaagaatga cgtcggcttg cggtacaccg tactgttcgt taatgccggt
    20401 atagttgacg atatctacca tcgccgcgct ggcttcggct ttgtagcgtt tgaccgcgct
    20461 gatttcctta ttgatgcgtt ggtgaaaata tttacgattg aaaagcgccg tgagcggatc
    20521 cgttaccgta agcgacttca gatgttcggt cttttcttcg agcttattat ttactttttt
    20581 tagttcttcg ttatgctgtt gaagctccgc tttcatgcgg tcgagtttat tgacttcttg
    20641 aagcaaacta tcgtaaagaa ctttattgcg caggagcgct tttactttga tcgccagttc
    20701 acgcggatcg taaggctgga tcacataatc atcaccgcct ttttcaaacc catgaacctg
    20761 atcaacaaac tctttaagat tggccgtaac aaaaacaatg ggcagatgcg cggaaatttt
    20821 tcgaatactt tcacaaatct caaatccgtc aatgcccggc aaacgtacgt ccagcaacat
    20881 gatatcggga tcgtgcgccg ttacggagcc caatgcatct tcgccgctgt gcgcttcaaa
    20941 aaacacatac tccggccgtt gcaacgtttt gcgcagggag tacacatggt cgggattatc
    21001 gtcaacgatt aagattttat acgtgcggct catggttttg tggcgttacg ctcagcctcc
    21061 atgatgcggt gaatttgctc cagtaatttt tcgcgcgaga atgcgctttt ctggaaaata
    21121 taataatggt tacccgaaag tttccgatgg tcttcctgcg taagcaattt gccggtcaat
    21181 acgagtaccg gtaaattttg cgtgatcgag tcactctgta aactcgataa aacggcaaag
    21241 ccgtctatct gcggcatcat cagatcgaga attaccaagt cgtaggattt gcgtcgggcg
    21301 gcttggatgg ctttttcacc gctggtcgcg acctctgata aaaaatcatc gctttccaaa
    21361 atttcttcga tcagttcggc ctgtgacaag tcgtcatcca cgatcaaaa
//
LOCUS       DAOQGF010000053        21287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1141_length_21287_cov_7.627967, whole genome shotgun sequence.
ACCESSION   DAOQGF010000053 DAOQGF010000000
VERSION     DAOQGF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_21287_cov_7.627967"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PK937_08795"
     CDS             <1..1816
                     /locus_tag="PK937_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH32708.1"
                     /translation="DPSKEVQIFAIDAAHRINAIDLIPMIIKKLKQKETRHKAMEALS
                     AFDAADIVPTLRTYLEFSRQYQEISLYIPRVFEMIASQECADIMLKNYDHAGPYLRLK
                     ILDSLAKIQFRNHDVELDRDLIEKWINLEFQEHRFFTQTLNAAKPFDGIAILIESVTH
                     IRHGVFERVFKLLGFLHDHKVITTVFLNLSNTDKRVQANAVEVLDNMLEGELRTSVVQ
                     IVNSHISHKFNEERDAIKFFDTLMAAYGKDHDWLKMCVEFVSTSPKFDEWDHNRAFAL
                     RKLIHDTQVSFERKEVLNIIQKVAILKRVSFFASLSGEYLANIALQMKSAKCHAGEAV
                     FHEGETGDSFYVVIKGKVLIHKGETRLVEMGPGQCFGEMAILDNEKRSASVTALEDTL
                     MFKLESEAFYDIIADRIEIAKGVIRMLTQRLRQQLAKVAAAGGGAPKPVIPQIEESAV
                     EEMIEGESANIASELDSQALLKRVLLLKEIDLFASLPDEDMVMLANTIEEVKYKKGQD
                     IFLEGDQGDAMYGIVSGSVLIHKGNKDLATLGEKKYFGEMAVLDNEPRSASVTAVADT
                     TLLKLSSDDFYSILFDKVEITRNIFAVLVQRLRTTNTK"
     gene            1830..2486
                     /locus_tag="PK937_08800"
     CDS             1830..2486
                     /locus_tag="PK937_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNH32709.1"
                     /translation="MSRIRVKICCISSREEARTAIQTGADALGLVSSMPSGPGVISDE
                     IIRDIARIIPPPIASFLLTSKQDAAAIVTQQKFCRTNTIQICDHLTNGTYEDIRKALP
                     GIAIVQVVHVTDESSINEAIRVSEFADAVLLDSGNQKLAVKELGGTGRKHDWRISARI
                     RAAIKVPLFLAGGLNVENVREAIETVQPFGIDLCSSVRTDGKLDKAKLERLFEVVHRI
                     "
     gene            complement(2577..>2936)
                     /locus_tag="PK937_08805"
     CDS             complement(2577..>2936)
                     /locus_tag="PK937_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH32710.1"
                     /translation="QSAYTLTVSGGLAQVTRGGEKGLAGIPQAVELLQNYPNPFNPST
                     MIRFGLPSENDVTLKIYNLMGQEVRTLFSGRMKAGQNEVLWDGRNAAGQQVSSGTYIY
                     RLQVGQEIISRRMNFIK"
     assembly_gap    2937..3036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3037..5993)
                     /locus_tag="PK937_08810"
     CDS             complement(<3037..5993)
                     /locus_tag="PK937_08810"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32711.1"
                     /translation="MKTMLRLVRYTTLLCFSVLAFAPVYGQQSVPSWISQDKSFKETS
                     KEFEQYWKGKDVHDKAVIRGKGFKQFNRYQWFWSTRTNAQGVFDPSLYLEAKAEKYSN
                     FPNSNFAPRRGMNRAMVNSSNWTTLGPQTAIPTNGGAGRVNCIVFDPSNSNTIYIGTP
                     GGGLWKTTNGGTSWTTNTDELGTLGVSHLAIDPTNTNTMYLATGDNDAGDTYSIGILK
                     SVDGGLTWSATGLTWTANQLYRISKISINPTSTSTLVATTSNGIYRSTDAGATWTQVQ
                     TGNFKDLDVNPSNYNEQYASFYGNGTASIYKSANGGSTWTKLTSGLPTTGVLRCAVAI
                     APSTPSTVYAIYCDNTSGGYGLYGIYKSTNSGSTWTQVKTSASPNVLGWNSNGGDTGV
                     GQGWYDLCITVSPTDANTVYTGGVNIWRSTNGGTTWTLNAQWQGSGAPYVHADHHAIE
                     FLPGSGTTLFSGNDGGFFKTTNSGTTWTDFSNGLGIHQYYRISAAKTSANTILGGAQD
                     NGTDRYNGTSWSRIIGGDGMECQVDYTNANIQYGALYYGDLYRTTTGGTPSNISDPTE
                     DGAWVTPYVLHPTTNTTMYYASASKINRSTNITNTNPTWTAISGTLGSASDPLTVLAV
                     SPSNGTTILTANAAGAWKGTASGSTWTFTSLSSSGLPAGVTSFAFHPTDVNTAWATIG
                     GFTAGSKVYKTTNGGTSWTNISGSLVNVPANCVAVHPSTPTDVYIGTDLGIFYSADGG
                     TTWEAYDTGLPNVIVTDLDIHVSAGKIRAATFGRGLWESPLNTSTPSNSVTVTAPNGG
                     ESLTGGSATTITWSSTGTMANVKLEYSVDGGTNWTVITASTTNDGSEAWTVPSSATTQ
                     GRVRVSDASASATNDMSNANFTITVSASNSVTLTAPNGGQSFTGGSATTITWSSTGTI
                     ANVKLEYSLNAGSTWTQITASTANDGSEAWTVPSSATTQGRIRVSDAANAATTDMSDA
                     NFTITTGTPPITA"
     gene            complement(6456..6683)
                     /locus_tag="PK937_08815"
     CDS             complement(6456..6683)
                     /locus_tag="PK937_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HNH32712.1"
                     /translation="MNISFQKIIYPLLASLTLGLAPFFPEPHIWKQIVNIANGTFTEP
                     LDWFDLAMHGAPWIWLIYALLKQSKKPVEPS"
     gene            6854..8254
                     /locus_tag="PK937_08820"
     CDS             6854..8254
                     /locus_tag="PK937_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32713.1"
                     /translation="MRFQKFLRVMLMLVIAGAFSVSAQEEGGNPTTGEGASSSGGFDF
                     QGPYDGSGGSQFTMPAGVTTIDGKSYISLRIQPELTLGKFGMGLDVPLLFSTENGKLR
                     TDEFKGGVGPLRMIRYIRYGVKHKDPVYARLGDLTGTTMGYGLIMYNYTNGISFEKRK
                     IGMNVDINYEQQYGFEAVYSDFDRVSIYGVRPYVKPFKQTDIPIIKSTEFGATYMTDR
                     DNKTAIQGMTVMGVDAGITVVDNGFLQIQPYTEFARMLKNDDLADSAASMGKSYGAGQ
                     GFALGSNFRFNIIANIFQLGVKLERRIFSSDFMPQYFDAVYEINKDAKAYKVVDAEAV
                     QGNYGEIYANLIGKIQFVGGISIPDNAKKSESAYIHIGMSAPNLIPKVIVSGTYDKGY
                     LDGVADAFRFRSKRSVAHLILGYEAYTMGPFVMQAGIDYKWTFIEKKDKSLKAVNYVM
                     PFVGITTKLPFGDQKK"
     gene            8369..9190
                     /locus_tag="PK937_08825"
     CDS             8369..9190
                     /locus_tag="PK937_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32714.1"
                     /translation="MLKHKVEPASDSAAAQDHRKVAWIDRGWYVAIEFEMASSGKLDQ
                     AIAIAKKNPFFVELVDERGMTWFRAIYFKKDFSKFQELYALAGPWKNTRFFLKGDEVK
                     KDDFETWYACYQQYWGHRKNLNLQDYCGTNKVSVYPDFIGCYGRNIVLRWQDPLTYYA
                     QYHTKLWYTFGKRQGNTFHLDKAAMRGFLKKQNAEYELCPCYGHESIDHYINKLPNDL
                     NPHIHTEWQFKEDFLKITANRAFIHYDITLSMMPEICPVNEKSYEKFMNRIFKES"
     gene            complement(9195..9971)
                     /locus_tag="PK937_08830"
     CDS             complement(9195..9971)
                     /locus_tag="PK937_08830"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNH32715.1"
                     /translation="MRFFFCRIVFIGAILWAPLSAQQIPIGEHTSYKLSYLSFSAASV
                     SFQVTGIVNINDRPCYRLVTDAQTTSFFSAFYDLHNTYETFVDTATGLPLRYTKNIAQ
                     KTLRQHLTINYDHDNQTAQYDGGKWDSVFTVNVQPQSHTLFSALFDLRRRTMYEGKQW
                     VYNLDNETEPWKIQVTVLSRENIPAAGKEYPAYKLQVRFIPVGEEKKRKHTDIVTRRL
                     VQSATKLYYWIEADAPHRGVQIEHETSPFSTYTILTGTGK"
     gene            complement(9977..10474)
                     /gene="moaC"
                     /locus_tag="PK937_08835"
     CDS             complement(9977..10474)
                     /gene="moaC"
                     /locus_tag="PK937_08835"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNH32716.1"
                     /translation="MKTSKKLSHVDHRGKATMVDVSGKSDTERSATAMARVTMSAAAF
                     DAIQNNAIKKGDVLTVAQLAGIMAAKKTSDLIPLCHPLNLSRVDVACFPEPDSLSIRI
                     EATCKLLGKTGVEMEALTAVTIAALTVYDMAKAMDKKMVIRDICLLEKSGGKSGIFRN
                     NKMKP"
     gene            complement(10471..11133)
                     /locus_tag="PK937_08840"
     CDS             complement(10471..11133)
                     /locus_tag="PK937_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH32717.1"
                     /translation="MTSEESKHDLISESLRELVRTPHLVRQALKNYQKLSDEEVINRF
                     QEGDLVAYDVIVARYKDQLINYVMSYVGEKKDAEDIVQDTFVKIYRFKQMYKRIAKFS
                     TWVYTVAGNLAKSELRKRKRQQLYPISSLGTDEKEFEAVETRVNADELTDSSVKKDLI
                     KKAIKTLPERYREVIRLREIENLSYEEIAEQTQLPIGTVRSRINRARVQLQSKLKFLV
                     EK"
     gene            complement(11130..12179)
                     /gene="holA"
                     /locus_tag="PK937_08845"
     CDS             complement(11130..12179)
                     /gene="holA"
                     /locus_tag="PK937_08845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNH32718.1"
                     /translation="MAREEKEKNASYNDVLKDVRKGNVAPMYLLYGSEVFLADQLADA
                     IVQAVFNAPKDDFNLHIFYGKEAPLDTVISTALGFPVMADRQVIILREADQWKLTKDK
                     RDDLKDLFPKFPDTTVFIAIGSGIDVRTNPWAWMRKNGIAIEFKPLRENEIPVWIDQF
                     LDERGKKITDQASLLLRTRVDLSLREVHNQIDKLCTYIGDRDTINEEDVEEAVGISRQ
                     YNIFEFCNAIGKRDLTKAMTVYEQMARFGGEPVYMIVMLTRHYHILLKMCEMRDQGTA
                     PSQIQSVMMTENRIFPNFFQTDYWPQAQKHKTSDVKLAFRCLLQADIQLKSSAVDKDM
                     LMQHLIYQLATSTAV"
     gene            complement(12192..12962)
                     /locus_tag="PK937_08850"
     CDS             complement(12192..12962)
                     /locus_tag="PK937_08850"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNH32719.1"
                     /translation="MSNALHDDIRRLLIQEQDLYGDEFTRDPAASGLWNHVSWDTPEY
                     STTDDERPPVVAERIETDMPKNIPLPTLSALQQTISTCTKCELCKTRTQTVFGSGNPN
                     ADIMFIGEAPGADEDEQGLPFVGRAGQLLTKMIEEPRSLNLPRSEVYIANILKCRPPG
                     NRKPLPAEVAQCEPYLITQIELIKPRIICALGATSANTLLKNNTPIGAMRGQTFDYHG
                     VPLLITYHPAALLRNPNWKKPAWEDMLTLRAMYDKLKK"
     gene            13154..13771
                     /locus_tag="PK937_08855"
     CDS             13154..13771
                     /locus_tag="PK937_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNH32720.1"
                     /translation="MRPITTMFWDLGGVVLTNAWDREQRIKVLNSHGITEKQHLDEFG
                     DRHREVAAGFETGHYSLDEYLDMTLFDMHHVAREPFKESMFGQSELLPGMDVLTEVAQ
                     AGRCFMATLNNESLPLNEYRIARFKLKQYFQVFCSSCYLGVTKPTKEIYETALRITHS
                     KPEECVFIDDREQNAEAAKKLGIHAIHYKNPDQLRKELRFLKIID"
     gene            13780..15618
                     /locus_tag="PK937_08860"
     CDS             13780..15618
                     /locus_tag="PK937_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNH32721.1"
                     /translation="MSFLRIDQVKHIIGFSQHVMRSVAILIFLSYSALQAQTVQCVAS
                     VEQSVIEQGTSFQLRIEVSGENLRITSLPQLPEMKGLEVMHQGAGESTSISIINGRRS
                     MSKSYDFILRAKDVGRWTIPPVAVGVDGKTVSSQALTIEVVPGGSISGSQRSEKTNDA
                     FLALTVSKKEAFQGEQITAELKIYSRIPITQYAPSRMPGFTGFWAEEFPMPQQIQSTR
                     EQINGVAYAVYTIKRVGLFATRSGLLTIDPSEIECELRIPRRSRNSLDDLFSPFGDPF
                     GQTATVKLQSEPVKLNILPLPKENAPSDFSGLVGQFDVKTAVDKNSLKTGEALTYTIT
                     ISGTGNIKSAEIPPTPFGDDFERYDPKSSETLNKNSNLVSGSKVFEYVGVPRLPRQVE
                     IPVFNLSYYDPVLKKYVTKSSGPHRVTIAQGTGGGIFSHNALSKEEIALLAKDIRFIK
                     TENLSEESYAPVYFRWWFIAGMIFPLLLWGTVYVWAIKHEELQKDGIGLRYRKASPLA
                     RKRLKAAEALMKSNQQDSFYTELSKTLNGLITDKLHVGENVYTREHITEILRDRNVEN
                     NLITEYMDCMHMCDEARFSPDGKSRARMDDAYERIRAAILNMDNAL"
     gene            15615..16367
                     /locus_tag="PK937_08865"
     CDS             15615..16367
                     /locus_tag="PK937_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32722.1"
                     /translation="MNRFLFWIVMSAAPVLWAQENDTLFAKAHAFYEARDFVAARDAY
                     QKLVDQGSVSGALFYNLANTYYRTKQFGMAVFYYEKALRLHPADEDVRFNLELTRLQL
                     KDKIVTPPRPEWVVWMIATLQAISLAEWGFVLVCIVNGFVVLLVAQRVRPQWRRVRTG
                     RILRMVWITLLCINASVFLWRAYSESNRVEAVILVPYVEIKNEPIETSTTVFILHEGT
                     KAVIRSERNEWLELRLDDGNVGWIKRPAVGIL"
     gene            16369..18762
                     /locus_tag="PK937_08870"
     CDS             16369..18762
                     /locus_tag="PK937_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNH32723.1"
                     /translation="MRSDTSVYPALKLTILYIVSILILDAVSPEIHWPINKAIVSSLV
                     VLTGIVLYWRFQNHFWGVIAVFFLVLGRMPDNQFQTHHVSSYLDLNQKVLVHGTVVND
                     PQINTQRTRFAVETDSVVFTRNLILPSSGKIQVVCFTDSTVRPAYGDRISIWGYLRSP
                     RGSRNPGEFDFAAYAAYHGLYGVMYAGDAANIRIEGHTSLNPVTEFFILPIKHFIIEL
                     NEKTLPPLHAAIASGLIVGDRSDIPQEILNAFSYSGAIHILSISGLHVVFISLLILGL
                     FSVLRVPYVWRIILTLISLFLYVWIADFVPPAVRAGIMTGVVLFGTLLERRTNIINSL
                     CVSLLIILFWDPLAIFDIGLQLSFLAVLSLVIIYPRLEAWCKKRRILTERTPSVLRWV
                     IMGLLVSIAAQIGTLPFTAYYFYKIPLTALIANVFISPISSAVMALGFLSAIVGSFSF
                     TLAQWYAHLNDYLIGLMIATARFSVKLPLAYTQFYRMDMIVLSVYYLSLAVWFFWDRK
                     LVRRWAVWVVIAALSVGVIFQNIPTSKKTVITFLDVGQGDACVVHLDDGRTIVIDAGD
                     QDPQFDYGERVVAPYLWKQGISSIDALILSHPHDDHIGGAGFLLNHFDVKRVFDPGQY
                     YPSAIYKNILNTIAEKSIDYQVLQKDRVVTFGPETRIRILHPGVSFVRSDESPPYDIN
                     NTSLVFVLEYGENRILFSGDAETETWDALSDSGMAMKADIVKIAHHGSSNGTTAKLIE
                     AASPAYAVISCGEGNKFGHPDLETVNAWRNAGTQVLRTDQWGAVQFWLDGKSIQRTR"
     gene            18766..21069
                     /locus_tag="PK937_08875"
     CDS             18766..21069
                     /locus_tag="PK937_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32724.1"
                     /translation="MLGYDLIVLLGFFVGAILYSVLFAETLKRQNKLKHEYVFLVFVA
                     ILTFWHGFQFLGMLIKQVFGRPALEIYSELRYISLYAIAFFPPLVVHLHASFLEYVLN
                     LINRRSRILAWFPYIMYAPILAILYFSQYTIIDGRRRILDEGMSFIPPYVFWLIVCLL
                     VSGFLSSRIIRLSRNEKWRQFFIVELIITIVISVLLVYFYFYGGTGNVRLDRSLKNMI
                     MISSLIPTGAMVYLLYKYPFYSTVARRRFLLITISGVFLVVFIMSSKALRKMAEENPE
                     LNVEFLEIVLVSMLFLVYEPTKYLVRRIAGQFALHEKSYFQSLIRKITEHIVSAANLA
                     ELTKAVRAGIVDALHVEEVGLYTLNKTVSGERTIYSIVHAYGDIKTFNLEKITRHIQK
                     GNGLYDARKMNIFHLRSAEVPYQIYLGIVLDNELVGILAIGKKKSNEDFEFEEKELLL
                     TVASQVAISIENIQLLQRRIELESKMFEADKLTSLGMLSTSIAHEVKNPLSSIKSIVQ
                     SMHDQKKKENTDTEAIQDLEIINEEIDRLSSVVNQLLRFARSDSSGMDRIDIIKIIDA
                     ILTISRQETRVRNIRIFTRYEHAPLVIRSRQGDLKEIIFNLIINAIQSMPEGGRLMIH
                     GAYVQMDANYFIGGAETEVGVFDKLYPDDPSAGWTEWSSEAVTAKKIEAIKKDPGTEI
                     ILPPEAGKTKCLRIAITDTGHGIPPERLGEIFKPFFTTKSEGTGLGLAIVKNKIQALG
                     GRLVLRSELQVGTSFEVYIPSESTSRS"
     gene            21066..>21287
                     /locus_tag="PK937_08880"
     CDS             21066..>21287
                     /locus_tag="PK937_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32725.1"
                     /translation="MSDIPVETTHENKSHIRILVVDDEKAARYGMLKALKTEGYKLSE
                     ADDGLSALNLMANNPFDIVITDVSMAGMNG"
BASE COUNT         5484 a         4678 c         5205 g         5820 t
ORIGIN      
        1 cgatccatcc aaagaagtgc agatatttgc cattgatgcg gcgcatcgca tcaacgcgat
       61 cgatctgatc ccgatgatca tcaaaaagct taaacaaaaa gaaacacggc ataaagcgat
      121 ggaggcgttg tcggcgtttg atgcggccga tatcgtaccg actctgcgaa cgtatctgga
      181 gtttagccgg cagtatcagg aaatcagttt atacataccc cgcgtttttg aaatgatcgc
      241 atcgcaggaa tgtgcggata tcatgcttaa aaattacgat catgccgggc cgtatctgcg
      301 tttgaaaata ttggactcct tagccaaaat acaatttcgt aaccacgacg tagagttaga
      361 tcgcgacctg atcgaaaaat ggatcaatct tgaatttcaa gagcatcgct ttttcacgca
      421 aacgttaaat gccgcaaaac cctttgatgg cattgcgatt ttgatcgaat ccgtaacgca
      481 tattcgtcac ggtgtgtttg aacgcgtatt taaactgctc ggttttttgc atgaccacaa
      541 ggtgatcact acggtttttc tcaacctatc caataccgac aaacgcgtac aggccaatgc
      601 cgtcgaagtg ctggataata tgttggaggg cgaattacgc acatccgtag tacaaatcgt
      661 caattcgcat atctcacaca agtttaacga agagcgggat gcgatcaagt tttttgatac
      721 cctgatggcg gcttacggca aagatcatga ttggctcaaa atgtgcgtgg agtttgtcag
      781 cacgtcgccg aaatttgacg aatgggatca caaccgtgcg tttgcgctgc gcaaactcat
      841 ccacgacacg caggtcagtt ttgagcgcaa agaagttcta aacattattc aaaaagtcgc
      901 gatcctcaaa cgcgtcagtt tttttgcctc cctttccggt gaatatcttg ccaatatcgc
      961 cctgcaaatg aaatccgcca agtgtcatgc cggtgaagcg gtatttcacg aaggtgaaac
     1021 gggcgattcg ttttatgtcg ttatcaaagg caaagtattg attcataaag gcgagacgcg
     1081 tttggtagaa atggggccgg gccaatgttt cggtgaaatg gccattttgg ataacgaaaa
     1141 acgatcggcc agcgtcacgg cattggaaga cacgttgatg ttcaaattgg aatccgaagc
     1201 gttttatgat attatcgccg atcgtatcga aattgccaaa ggcgtgatcc gcatgcttac
     1261 gcagcgtttg cgccaacaat tggccaaagt cgctgccgcc ggcgggggcg ctcccaaacc
     1321 ggtcataccg caaatcgaag aaagcgccgt cgaggaaatg atcgaaggcg aaagcgccaa
     1381 tatcgcttcc gaattggata gccaggcttt actcaagcgc gtgctgctgc tcaaagaaat
     1441 agatctcttt gccagtttgc cggatgaaga tatggtcatg ctggccaata ccatcgaaga
     1501 agtgaagtac aaaaagggac aagatatttt cttagaaggc gatcagggcg atgccatgta
     1561 cggtatcgtt agcggatccg ttttgatcca caaaggcaac aaagatttgg ccaccttggg
     1621 tgaaaagaaa tatttcggtg aaatggccgt gcttgataac gaaccgcgtt ccgcttcagt
     1681 gaccgctgtt gccgatacga cgcttttaaa actgtcatcg gatgattttt attcgatctt
     1741 gtttgataag gtcgaaatca cccgtaatat tttcgccgta ttagtacaac ggcttcgtac
     1801 caccaatacc aaatagatcg gaattttttt tgtctcggat acgagtgaaa atctgctgca
     1861 tttcttcacg cgaagaggcg cgtacggcga ttcaaaccgg tgcggatgca ttggggttgg
     1921 tttcgtccat gccgagcggg ccgggggtta tttccgatga aataatccgg gatattgcgc
     1981 ggatcattcc ccctccgata gcttcgtttc ttttgaccag caaacaagat gccgccgcga
     2041 tcgtaacgca acaaaagttt tgccgaacca atacgattca aatatgtgat catttgacga
     2101 acggtactta cgaagatatc cgaaaagctt tgccggggat cgctatcgta caagtcgtac
     2161 atgtaaccga cgaatcatcc atcaacgaag cgatacgcgt atcggagttt gccgatgcgg
     2221 tattactgga ttcgggaaat caaaaattgg ccgtcaaaga gcttggcgga actggccgta
     2281 aacacgactg gcgaatcagc gcacgcatcc gagccgcgat caaagttcct ctgtttttgg
     2341 ccggcggact caatgttgaa aatgtacgcg aagctatcga aacggtgcaa ccttttggta
     2401 tagatctgtg cagcagcgta cgtaccgacg gaaaattaga taaagctaag ttagaacgat
     2461 tgtttgaggt tgtacatcgc atctgatcaa aacaaactca ggttctcagc aggtttataa
     2521 aaaaagcccg atctgaaatt tcagaccggg cttcgtattt gttcgagcgt gatgaattat
     2581 ttgataaagt tcatccgtcg tgaaatgatt tcttgtccga cttgcaggcg atagatatag
     2641 gtaccgctgg aaacctgctg gcctgcggca ttgcgaccat cccacaaaac ctcattttga
     2701 ccggctttca tacgtccgct gaacagcgta cgtacttctt gtcccatgag attgtagatt
     2761 ttgagcgtga cgtcgttttc agacggtaaa ccaaaacgga tcattgtgga cgggttgaat
     2821 gggttgggat aattctgcag aagttcgact gcctgcggaa taccggccaa tcctttttcg
     2881 ccgcctcggg ttacttgagc caatccgcca ctcacggtca aggtataagc cgattgnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctg ccgtaatcgg cggcgtaccg
     3061 gtagtgatgg taaaattagc atcactcata tctgtcgtgg cggcatttgc ggcatcactt
     3121 acgcgaatgc gcccttgggt ggttgctgaa ctcggaacag tccacgcttc ggagccgtca
     3181 ttggctgtgg aagccgtgat ctgtgtccat gtcgagccgg cattgagcga gtattctagt
     3241 tttacattgg cgatggttcc cgtggaggac cacgtgatcg tcgtcgcgct accgccggtg
     3301 aacgattggc cgccattggg cgctgtcaag gtgacactgt tggatgcgct taccgtgatt
     3361 gtaaaattag cattactcat gtcattggtc gccgaagcgg atgcatcact gacacgcacg
     3421 cgaccttgtg tcgtcgctga agaaggaacg gtccatgctt cggaaccgtc attggtcgta
     3481 gaggccgtta tcactgtcca attggtaccg ccatccacgg aatattccaa tttgacatta
     3541 gccatcgttc ctgtcgagga ccatgtgatc gtcgttgcac tgccgccggt aagcgactcg
     3601 ccgccattgg gcgctgtgac agttaccgaa tttgaaggtg tggacgtatt aagcggcgat
     3661 tcccataatc cgcgaccaaa cgtcgctgca cgaattttac cggcgctcac atgaatatcc
     3721 aaatcggtaa cgataacatt gggtaatcct gtatcatagg cttcccatgt cgttccgccg
     3781 tccgcagagt agaatatacc aaggtctgtt ccgatataga catcggtcgg ggtgcttgga
     3841 tgtacggcga cgcagttagc aggtacattg accaaagaac cggaaatatt tgtccacgaa
     3901 gtaccgccat tggtggtttt ataaactttg ctacctgccg tgaaaccgcc gatggtagcc
     3961 cacgcggtat ttacatccgt agggtgaaat gcaaatgagg taacaccagc cggcaaaccg
     4021 ctgctcgaca agcttgtaaa tgtccaggtt gagccgctgg ctgttccttt ccaagcgcct
     4081 gccgcattag ctgtgaggat cgtcgttccg ttggaaggcg atacggctaa tactgtcaaa
     4141 ggatcgcttg cggaaccgag ggtaccgctg atagctgtcc acgtcgggtt cgtattggtg
     4201 atattagttg agcggttaat tttgctggcg gaagcgtagt acatcgtcgt attcgtcgtc
     4261 ggatgcaata cgtacggcgt cacccaagcg ccgtcctctg taggatcgga aatattggac
     4321 ggggtaccgc ctgtagtcgt gcgatacaga tcgccgtagt acaatgcacc gtattgaata
     4381 ttggcgttgg tataatctac ctgacattcc ataccgtcgc cgccaatgat gcgtgaccag
     4441 ctcgttccgt tataacgatc cgtgccgtta tcctgtgcgc caccgagtat cgtattagcg
     4501 ctggttttgg ctgcactgat acgataatac tgatggattc cgaggccgtt actaaaatcg
     4561 gtccatgttg ttccgctatt ggtagttttg aagaatccgc catcgttacc ggaaaacaaa
     4621 gtggtaccgc tgccgggtaa aaattcgatc gcatgatgat cggcatgtac gtaaggagcg
     4681 cctgatcctt gccactgagc attcagtgtc catgttgtac caccattggt agaacgccag
     4741 atgtttacac cgccggtata aaccgtattg gcatctgtcg gggaaacggt gatgcagaga
     4801 tcataccagc cttgaccgac accggtatca ccgccgttac tattccatcc gagtacattg
     4861 gggctggcgg aggttttgac ttgtgtccac gtcgaaccgc tattggtaga tttataaata
     4921 ccatagagac cataaccgcc cgaggtgtta tcacaataaa tcgcatacac ggtggacggt
     4981 gtcgaaggcg cgatggctac cgcgcaacgc aatacacctg tcgtcggaag gccggaagtc
     5041 agtttagtcc atgtcgagcc gccgttggcg gatttataaa tagaagcggt gccgttgccg
     5101 taaaaactgg cgtactgttc attatagttg gatggattga catccagatc tttgaagtta
     5161 ccggtttgta cttgtgtcca agtggcgccc gcatccgtcg aacgataaat accgttgctg
     5221 gtcgtagcga cgagcgtgct tgtggacgta ggattgatgc tgatttttga aatacgataa
     5281 agttgattgg ctgtccatgt caatcctgtc gccgaccagg tcaagccgcc gtccacggat
     5341 ttgagaatac cgatggaata cgtatcgccg gcgtcgttgt caccggttgc aagatacatc
     5401 gtattggtat tggtagggtc aatcgctaaa tgcgacacgc ctaaagtacc caattcatcc
     5461 gtattggttg tccacgatgt accgccattg gtggttttcc agagaccgcc gcccggcgta
     5521 ccgatataaa tcgtgttgct gttgctcgga tcaaaaacga tgcaattcac acgtcctgcg
     5581 ccaccgttcg tcggtatggc ggtttgcgga ccgagcgtgg tccagttgga actgttgacc
     5641 atcgcgcgat tcataccgcg gcgcggtgca aaattggaat ttggaaaatt ggaatatttt
     5701 tcggctttgg cttcaaggta caaactggga tcaaaaacac cttgcgcgtt ggtacgcgta
     5761 ctccaaaacc attgataacg attaaactgt ttgaatcctt tgccgcgaat cacggctttg
     5821 tcatgaacat cttttccttt ccagtactgt tcgaattctt tcgatgtttc tttaaacgat
     5881 ttgtcctgtg aaatccagga tggtacggat tgttgtccat acaccggcgc aaaggccaga
     5941 acactaaaac ataatagtgt agtataacgc actaggcgta acattgtttt catgggttat
     6001 tctctcctca taaccatgcc gttggaaaat ttgttattta gtaaaaatga agtaatgaaa
     6061 aatgaaacgg tatccgtacc gttcagggcg ggcaaaaaag ggacggcctt tgtaaagccg
     6121 gaatgcggtg atgaacaaca agcctcatac tcctcctcat aagagtaagg tttgtcaata
     6181 ccgacaatct gcgttaatta atttacgcag cagaagaatt ttaaatcgta acgccaaagc
     6241 aaaggaaatg cttttgcgga ttggtcacag gcgaccgaag tgaataaaaa aatcagaaag
     6301 gaataacttg aaaagataag aacggctcaa gtataggctg tctaaaaaaa gaagtaaaaa
     6361 aaaaaaataa aaaaatcact gtagaaccgc tacaggcaag ggtttgtaga atccgtgtga
     6421 tatattacaa agaaattata tcagatttaa aaattctatg acggctcaac gggcttcttg
     6481 gattgtttta agagcgcata gatcagccat atccacggtg cgccatgcat ggccaaatca
     6541 aaccaatcca gcggctcggt aaaagttccg ttggcgatgt taacgatttg tttccaaata
     6601 tgaggttccg gaaagaaggg tgctagaccg agtgtcagac tggcgagtaa aggataaata
     6661 attttttgaa atgaaatatt catacgtcaa tccgataata gaattatgga gtcaatatat
     6721 gatcttagcc tgtatgctca aagattttat tttgtcggca tatattttca agtttgcaaa
     6781 tagacgatat tcgttttacc ttagcccgtt taatcggatg aatggcgtac atttaataat
     6841 gtcaggaggt acaatgcgtt ttcaaaagtt cttacgtgtc atgctgatgt tggttatcgc
     6901 gggagcgttt tccgtgtcag cacaagaaga aggcggcaat cctacaaccg gtgaaggcgc
     6961 ttcttcgtcg ggcggctttg attttcaagg accttatgac ggttccggcg gaagtcaatt
     7021 taccatgccg gcgggtgtga caacgattga cggaaaaagt tatatcagtt tgcgtattca
     7081 acccgaactg acgctcggaa aattcggtat gggtttggac gtgcctctgc tttttagcac
     7141 ggaaaacggc aaactgcgta cggatgaatt taaaggcggc gtcgggccgt tgcgtatgat
     7201 acgctatata cgttatggcg taaaacacaa agatcctgtc tatgcgcggt tgggtgatct
     7261 gaccggaacg acgatgggtt atggtctcat catgtataat tataccaacg gaatcagctt
     7321 tgaaaaacgt aagatcggta tgaatgtaga tatcaattac gaacagcaat acggtttcga
     7381 agcggtgtat agtgattttg atcgcgtgag catttacggt gtgcgtccgt acgtcaaacc
     7441 tttcaaacaa acggatatac cgatcatcaa aagcacggaa ttcggcgcaa cgtatatgac
     7501 ggatcgtgat aataagacgg ccattcaagg tatgaccgtc atgggtgtag atgccggtat
     7561 cacggttgta gataatggtt ttctacaaat acaaccttat acagaatttg cacgcatgtt
     7621 gaaaaacgat gatctcgctg actccgccgc gagcatgggc aagagttacg gcgcggggca
     7681 aggctttgcg ttgggaagta atttccgatt taatatcatc gccaatatat tccagttagg
     7741 tgtaaaatta gaacgtcgta ttttcagcag tgactttatg ccgcagtact ttgacgcggt
     7801 ctatgaaatc aataaagacg ctaaggctta caaagtcgta gacgccgaag ccgtccaggg
     7861 caactacggt gaaatctatg ccaatctgat cggcaagatt caatttgtag gcggtatcag
     7921 tattcccgac aatgctaaaa agtccgagag cgcttatatc catatcggaa tgtccgcacc
     7981 taatctgatt cctaaagtta tcgtatcggg aacgtatgat aaaggatact tagacggagt
     8041 tgcggatgca tttcgttttc gcagcaaacg cagcgtagcc catttgatcc tcggttatga
     8101 agcctatacg atgggacctt ttgtcatgca agccggtatt gactacaaat ggactttcat
     8161 cgagaaaaaa gacaaatccc tgaaggctgt taattatgtg atgccttttg tgggcattac
     8221 gaccaaattg cctttcggcg atcagaaaaa atagtttgaa agaataaatg gatttcaaag
     8281 gctgcctgtc gaaaggcagc ctttttttgt tgaaggtttt ttattatact caaagtataa
     8341 gtcatacgga atcatattaa acaggaatat gcttaaacat aaagtcgaac cggcttcgga
     8401 ctctgccgcc gctcaagatc atcgcaaagt agcctggata gaccgcggtt ggtatgtggc
     8461 tatcgaattt gagatggctt cgtccggtaa gctcgatcag gcgattgcca tagccaaaaa
     8521 aaatccattt ttcgtagagc tggtagatga gcgcggcatg acgtggtttc gtgcaattta
     8581 tttcaaaaaa gatttctcca agtttcagga attgtatgcg ctggcagggc cgtggaaaaa
     8641 cacgcgtttt tttctcaaag gcgatgaagt caaaaaagac gattttgaaa cgtggtatgc
     8701 ctgttatcag caatattggg gtcatcggaa gaacctaaac ctgcaggact actgcggtac
     8761 aaacaaagtc agcgtttatc ctgattttat cggctgctac gggagaaata tcgtactacg
     8821 gtggcaagac ccgctgacat actatgcgca gtatcatacc aaactttggt acacattcgg
     8881 aaaaaggcag ggtaatacgt ttcatttgga taaagcggcg atgcgtggat ttttgaaaaa
     8941 acaaaatgcc gaatacgaac tatgcccttg ctacggtcat gaatccattg accactatat
     9001 caataagctt cccaacgacc ttaatccgca tattcatacc gaatggcagt ttaaggaaga
     9061 tttcctgaag attacggcca atcgtgcgtt tattcattat gacattacgt tgagcatgat
     9121 gcctgagatt tgcccggtca atgaaaaatc ctatgaaaaa ttcatgaata ggatattcaa
     9181 agaatcgtaa tgatctattt accggtaccg gtcagaatag tgtaggtgct aaacggggac
     9241 gtttcatgtt cgatctgcac acctctatgc ggcgcatccg cctcaatcca ataatatagt
     9301 tttgtcgcac tttgtaccaa tcgccgcgta acgatgtcgg tatgctttcg ttttttttct
     9361 tcgccgacgg ggatgaatcg aacttgtaat ttgtaagcgg gatactcttt tcctgccgcc
     9421 gggatatttt cacgcgataa aacggtgacc tgtatcttcc acggttcggt ctcattatcc
     9481 aaattgtaaa cccattgttt tccttcgtac atcgtccgtc gtcgcaaatc aaatagcgct
     9541 gaaaataaag tatgcgattg cggttgcaca tttacggtaa acacggaatc ccattttccg
     9601 ccgtcatact gcgccgtttg gttatcatga tcgtaattta tagtcaaatg ctggcggagg
     9661 gttttctgcg cgatattttt ggtataacgc aaaggcagtc cggttgcggt atctacaaag
     9721 gtttcatacg tattatgcaa atcataaaac gccgaaaaaa aagatgtggt ttgtgcatcc
     9781 gtaacgaggc gataacatgg gcggtcgttg atattgacga tgccggttac ctgaaacgaa
     9841 accgaagcgg ccgaaaatga aaggtatgaa agtttatagg atgtatgttc cccgatgggt
     9901 atttgctggg ccgaaagtgg cgcccataaa atcgccccga taaaaacaat acgacagaaa
     9961 aaaaaacgca tcagaattaa ggtttcattt tgttgtttcg gaaaatcccg cttttgccgc
    10021 cgcttttttc gagcaggcat atatcgcgga tgaccatctt tttatccatc gctttggcca
    10081 tatcgtaaac ggtcaacgcc gcaatcgtta cagccgttag ggcctccatt tcaacacctg
    10141 ttttacccag gagtttgcac gtcgcttcaa ttcgaatgga caatgagtcc ggttcaggaa
    10201 aacaggcgac atccacacgg ctcaggttaa gcggatggca caacggaata agatccgaag
    10261 tttttttggc cgccatgatt ccggcgagtt gtgctaccgt gagtacatcg ccttttttaa
    10321 tggcgttgtt ttgtatggca tcaaatgccg ccgcgctcat cgtaacgcgc gccattgccg
    10381 ttgcactacg ttcggtgtcc gattttccgg aaacatcgac catagtcgct ttgccgcgat
    10441 ggtccacgtg ggaaagtttt tttgacgttt tcatttttcc accaaaaatt tcaatttgga
    10501 ttgcagttgt acacgggcgc gattgatgcg cgaacgtacg gtgccgatcg gaagttgcgt
    10561 ttgttccgca atttcttcgt acgaaaggtt ttctatctca cgaaggcgaa tcacttcgcg
    10621 gtatcgctcc ggcagtgttt taatcgcttt tttgatcaaa tcttttttca cggaggaatc
    10681 cgtgagttca tccgcattga cccgcgtttc gaccgcttca aattcttttt catccgtccc
    10741 caggctggaa atcggataca gttgctggcg tttgcgtttg cgcagttcgc ttttggcaag
    10801 attgccggcg acggtataga cccatgtaga aaacttggca atgcgtttat acatttgttt
    10861 gaaacggtaa attttgacaa acgtatcctg cacgatatct tcggcatctt ttttttcgcc
    10921 gacataactc atcacataat tgatcaactg atctttgtaa cgggccacga tcacgtcgta
    10981 agctacgaga tcgccttcct gaaaacggtt gatcacttct tcgtccgaca atttttgata
    11041 attttttaat gcctgtcgta ccaaatgcgg cgtacgcacc agttcgcgga ggctttcgct
    11101 tatgagatcg tgtttggatt cttcggatgt catacggcgg tactcgtggc gagctggtag
    11161 atcagatgtt gcatgagcat gtctttgtca acggcgctgg acttaagctg aatatccgcc
    11221 tgcaaaaggc aacggaaggc gagtttgaca tccgatgttt tatgtttctg tgcctgcggc
    11281 caatagtcgg tttgaaaaaa attgggaaaa atccggtttt cggtcatcat cacggattgg
    11341 atctgtgaag gcgcggtgcc ctgatcacgc atttcgcaca ttttgagaag aatatgatag
    11401 tgccgcgtga gcataacgat catgtagacc ggttcaccgc caaaacgcgc catttgttcg
    11461 tacaccgtca tggctttagt cagatcgcgt ttaccgatgg cattacaaaa ttcaaaaata
    11521 ttgtattggc gggaaatgcc gacggcctct tcgacatctt cttcattgat cgtatcgcga
    11581 tcgccgatat acgtgcataa tttatcaatc tgattatgca cctctctgag cgatagatct
    11641 acgcgcgttc gaagcagcaa cgacgcctga tccgtgattt tttttccgcg ttcatcaaga
    11701 aactgatcga tccatacggg aatttcattt tcgcgtaacg gtttgaattc gatggcgatg
    11761 ccgtttttcc gcatccatgc ccagggattg gtacgcacat cgatgccgct gccgatcgct
    11821 ataaatacgg tagtatccgg gaacttgggg aatagatctt tgaggtcgtc gcgtttatct
    11881 ttagtcagtt tccactgatc ggcttcgcgc aaaataataa cttgccgatc ggccatgacg
    11941 ggaaaaccca aggccgtgct gatcaccgta tccagcggcg cttctttgcc gtaaaaaata
    12001 tgcaaattaa agtcgtcttt gggggcatta aaaacagctt gaacgatcgc atcggccaat
    12061 tgatcagcga gaaaaacttc gctgccgtag agcagataca tcggagcgac gtttcctttg
    12121 cgcacgtctt tgaggacatc attgtacgag gcgttttttt ctttttcttc gcgggccatg
    12181 agaagtggtg tttatttttt caatttgtca tacatggcgc ggagtgtgag catatcttcc
    12241 caagccggtt ttttccaatt ggggttgcgc agcaaagcgg ccggatggta cgttatgagt
    12301 aaaggtacgc cgtgataatc aaacgtttgt ccgcgcatcg caccaatcgg cgtgttgttt
    12361 ttcagcaacg tattggcgga tgtcgcaccg agtgcacaaa taatacgcgg tttgatcaat
    12421 tcgatctggg tgatgagata aggttcgcac tgcgcgactt cggcgggtaa aggtttacgg
    12481 ttgcccggcg gtcggcactt caggatattg gctatgtaca cttccgaacg tggcagattg
    12541 agagaacgcg gctcttcgat catcttcgtc aaaagctgac cggcgcgacc cacgaaaggt
    12601 aaaccttgtt catcttcatc ggcacccggc gcttcaccga taaacatgat gtcggcattc
    12661 ggattgccgg agccaaacac tgtttgcgtg cgcgttttgc acaattcgca tttggtgcag
    12721 gtactgatgg tttgctggag agcggataac gtcggaaggg ggatgttttt gggcatgtcg
    12781 gtttcgatcc gctcggcgac aaccggcggc ctttcatcat cggtggtaga atattccggc
    12841 gtatcccatg agacatgatt ccaaagaccg gaagcggcgg ggtcgcgggt aaattcatcg
    12901 ccgtacaaat cctgttcttg aatcaacaat ctacggatat catcgtgcaa ggcattactc
    12961 atgatcacac tataaccata ttccgctttt ttatcaagcg aaacgccatg ccaggaaaga
    13021 ggaacgttaa tgacggaaac ggatacgctc cttagtaaat tcttttagca tataggtatt
    13081 gaaaaaagcc tgtgcactgt ataagttccc tcgtttcaat tttaaaaaaa cacttaattg
    13141 aaagaacaca actatgcgac ccatcacgac gatgttttgg gatcttggcg gagtagtttt
    13201 gaccaatgct tgggatcgtg agcagcgaat caaagtgctg aattcgcatg gaattacgga
    13261 aaaacaacat ttagacgaat tcggcgaccg tcatcgtgaa gtcgccgcag gatttgagac
    13321 cgggcattat tcgctggatg aatatcttga tatgacgctc ttcgacatgc atcacgtcgc
    13381 ccgcgagccg tttaaagaat ccatgtttgg tcaatccgag cttctaccgg gaatggatgt
    13441 gctgacggaa gtcgcgcagg cgggccgttg ttttatggcc acattgaaca atgaatcgct
    13501 gccgctgaac gaataccgca tcgcgcgttt caagctcaaa cagtattttc aggtattttg
    13561 ttcttcgtgt tatttgggtg ttaccaaacc gaccaaagaa atttacgaaa cagcgctccg
    13621 gattacacat agtaaacctg aagaatgcgt ttttatagat gaccgtgaac aaaacgccga
    13681 ggcagccaag aaactgggca tacacgcgat tcattacaaa aatccggacc aactgcgcaa
    13741 agaattgcgt tttctcaaaa ttatagattg aatcctattg tgagcttttt gcgtatagat
    13801 caggtaaaac acatcatcgg ctttagtcaa cacgtcatgc gaagtgtagc gattttaatt
    13861 tttttatcgt attcggcgtt gcaagcgcag accgtccaat gcgtggcttc tgtagagcag
    13921 tccgtcatcg aacaaggtac gtcatttcaa ctgcgcatcg aagtgtcggg tgaaaacctg
    13981 cgcatcacat cgcttcccca attgcctgaa atgaaaggcc ttgaggtcat gcatcagggt
    14041 gcgggcgaat ccacgtccat atctattatc aacggtcgtc gctccatgtc caaatcgtac
    14101 gattttattt tgcgtgcgaa agatgtgggt cgctggacga tccctcctgt ggccgtgggt
    14161 gtggacggaa aaacggtgtc ctcgcaggcg ctcacgatcg aagtcgttcc gggcggaagc
    14221 atatccggtt cccagcgttc ggaaaaaact aacgatgcgt ttctcgcatt gacggtttcc
    14281 aaaaaagaag cgttccaagg tgaacaaatt acggccgaac taaaaattta cagccggatt
    14341 ccgatcactc aatacgcgcc aagccggatg ccgggtttta caggattttg ggctgaagaa
    14401 tttcccatgc cgcaacaaat acaatctacc cgtgaacaaa tcaatggcgt ggcgtatgcg
    14461 gtatatacga tcaagcgggt agggcttttt gcaacgcgat ccggtttgct aacgatagat
    14521 ccgtcggaaa tcgaatgcga actgcgcatt ccgcggcgca gcagaaactc gttggatgat
    14581 ttattttctc cgttcggcga tcctttcgga cagacagcta cggtcaagtt gcaatcggaa
    14641 cctgtgaagc tgaatattct cccgttgccc aaagaaaatg cgccgtcgga tttttcgggt
    14701 ttggtcggac aatttgacgt aaaaactgcg gttgacaaaa acagtttgaa aacgggtgaa
    14761 gcgctgacgt atacgatcac tatatcgggg acaggaaata ttaaatccgc cgagattcca
    14821 ccgacgcctt tcggtgatga ttttgagcgg tacgatccga agtcgtccga aacgctgaat
    14881 aaaaattcta atctcgtcag cggcagtaaa gtttttgaat atgtcggtgt gccgcggcta
    14941 ccgcgccaag tggagatacc ggtttttaat ctctcttatt acgatccggt tttgaaaaaa
    15001 tacgttacta aatcatcagg tcctcaccgt gtgaccatag cacaaggtac agggggcggt
    15061 attttttcgc ataacgcgct gagtaaagag gaaatcgcgt tgttggcgaa agacattcgt
    15121 tttatcaaaa cggaaaacct ttcggaagaa agttatgcgc cggtttattt tcgctggtgg
    15181 tttatcgcgg gaatgatatt tccgcttctt ttgtggggta cggtatatgt gtgggcgatt
    15241 aaacacgaag aattgcaaaa agacggtatc ggtttgcgat atcgcaaagc cagtcctctg
    15301 gcgcgtaaac gtctcaaagc ggctgaagcg ttgatgaaat ccaatcaaca ggattcgttt
    15361 tataccgaac tttccaaaac attaaacggc cttatcaccg ataaattgca tgtcggtgaa
    15421 aatgtgtaca cccgcgagca cataacagaa attttgcgcg atcggaatgt ggaaaacaat
    15481 ttgataacgg aatatatgga ctgcatgcac atgtgtgatg aagcgcgttt ttcacccgac
    15541 gggaagtcgc gcgcacggat ggatgatgcg tacgagcgta ttcgtgcagc tattttaaat
    15601 atggacaacg cgctatgaat cgttttttgt tttggatcgt gatgagcgct gcaccggtgt
    15661 tatgggcaca ggaaaatgat accctgtttg cgaaggcgca tgctttttat gaggcgcgtg
    15721 attttgtagc ggcccgtgat gcataccaaa aacttgttga tcaaggttcg gtttccggcg
    15781 cgttgtttta taatttagcc aatacctatt accggacgaa acaatttggt atggccgtgt
    15841 tttattacga aaaagcatta cgtttgcatc ctgccgacga agatgtacgg tttaatctcg
    15901 aattgacacg actgcagctt aaagataaaa tcgtaacacc gcccagaccg gaatgggtcg
    15961 tatggatgat cgctacgctt caagcgattt cgcttgccga atggggtttt gtgttggtct
    16021 gtattgtaaa cggatttgtc gttttattgg ttgctcaaag agtacgcccg caatggcgaa
    16081 gggtgcgcac gggccggata ctgcgcatgg tatggatcac acttctttgt atcaacgcat
    16141 ctgttttttt atggcgtgcc tacagcgaat ccaatcgggt cgaagccgtg attttggttc
    16201 cttatgtgga aatcaaaaat gaaccgattg aaacatcaac gaccgtattc atactgcacg
    16261 aaggcacgaa agctgttatt cgatcggagc gcaacgaatg gttggaactg cgtttggatg
    16321 acggaaacgt gggatggata aaacgaccgg ctgtcggcat tctctgatat gcgaagcgat
    16381 acatcggttt atcccgcgct caagcttacc attctctata tcgtttccat acttattctc
    16441 gatgcagtat ccccggaaat ccattggccg ataaataaag ccattgtctc ttcgcttgtt
    16501 gttttgaccg gtatcgtttt gtattggcgc ttccaaaatc acttttgggg tgtcatcgcc
    16561 gtgttttttc ttgtattggg ccgtatgccc gataaccaat ttcaaacgca tcacgtttca
    16621 tcctaccttg atttgaatca gaaagtattg gtacacggga ctgtcgtcaa cgatccgcaa
    16681 atcaacactc agcgaacacg ttttgcggtg gaaaccgact cggtcgtttt tacccgaaat
    16741 ttaattttac cgtcgtctgg aaaaatccag gttgtatgct ttactgattc gaccgttcgc
    16801 ccggcgtatg gcgatcgcat ttcgatttgg gggtatctgc gttctccgcg cggaagtcgt
    16861 aatccgggag agtttgattt tgcggcgtat gctgcatacc acggattata tggtgtcatg
    16921 tatgccggcg acgcggccaa tattcgcatc gaaggtcata cgtcactgaa tccggtgacg
    16981 gaatttttta tcttaccgat caaacacttt atcattgagc tcaatgaaaa aacgcttccg
    17041 ccgctgcatg ccgccatcgc cagtggtctt atcgtcggtg atcgcagcga tataccgcag
    17101 gaaattctta atgctttttc gtatagcggc gcgattcaca ttctttcgat cagcggttta
    17161 catgtggttt ttatttcgct tttaatcctt ggactattta gtgtcttacg tgtgccgtac
    17221 gtatggcgta ttattcttac gctgatcagt ctttttttgt atgtgtggat agccgatttt
    17281 gtgccgccgg cggtgcgtgc cggtatcatg accggcgtcg tgttattcgg tacattattg
    17341 gaacgccgaa cgaatattat caattcactg tgcgtttcgc tgctgatcat tctcttctgg
    17401 gatcctttgg cgatttttga tatcggatta caactctcat ttttagcggt gttgtcatta
    17461 gttattatct atccgcgtct ggaagcatgg tgcaaaaagc gacgaattct cacggaacgt
    17521 accccgtcgg tgttgcggtg ggtcattatg gggttattgg tttcgatagc ggcacaaatc
    17581 ggtactttac cgtttacggc atattatttt tataaaattc cgcttactgc gttgatagca
    17641 aatgttttta tatcaccgat ttcatcggcg gtgatggcgt tgggattttt gtccgcgata
    17701 gtcggttcct tttcgtttac gcttgcccaa tggtatgccc atctcaacga ttatctgatc
    17761 ggattgatga tcgccacggc ccggttttcc gttaaacttc ctctcgccta cacgcagttt
    17821 taccgcatgg atatgatcgt attgagcgtt tattatttat cgctggccgt gtggtttttt
    17881 tgggacagga aattggttcg ccgctgggcc gtttgggttg ttattgcagc tttaagtgtt
    17941 ggggtgattt tccaaaatat accgacttct aaaaaaacag tgattacatt tttagatgtg
    18001 ggtcaaggcg atgcgtgtgt cgtacatctg gatgatgggc gtacgatcgt gatcgatgca
    18061 ggagatcagg atccgcagtt tgattatggc gagcgggttg tcgcgcctta tttgtggaaa
    18121 caagggattt cttcgattga tgcgttgatt ttatcgcatc cgcatgacga tcacatcggc
    18181 ggcgccggat ttttactcaa tcattttgat gtaaaacggg tttttgatcc gggtcaatat
    18241 tatccgtcgg cgatttataa aaatattttg aataccattg cggaaaaatc tatagattat
    18301 caagttttgc aaaaggaccg ggttgttacc tttggtccag agacgcgaat acgtatactg
    18361 catcccgggg tatcgttcgt gcgaagcgat gagtcgccgc cgtatgatat caataatacg
    18421 tcgcttgtat ttgttttgga atacggcgaa aatcggattt tatttagcgg ggatgcagag
    18481 accgaaacgt gggatgcgtt gtccgattcc gggatggcga tgaaggccga tattgtcaaa
    18541 atcgcacatc acggttcttc caacggtacg acggcaaaat tgatcgaggc cgcgtctccg
    18601 gcatacgcgg taatatcttg cggagaagga aataaattcg gtcatcctga tttggaaacc
    18661 gtaaatgcct ggcgcaatgc cggtacgcag gtcttacgaa cggaccaatg gggcgctgtt
    18721 cagttttggc tcgatggtaa atccatacaa cgcactcggt aaactatgtt aggctacgat
    18781 cttatagttc tgctcggttt ttttgtcggc gcgatacttt atagcgtgct gtttgccgag
    18841 actctcaagc gtcagaataa actgaagcat gaatatgtgt ttctggtatt cgtggcgata
    18901 cttacgtttt ggcacggatt tcagtttctc ggaatgctca tcaaacaagt atttggccgc
    18961 ccggcgctgg agatttactc cgaactccgt tacatctctt tgtatgcgat agcgtttttt
    19021 cctccgctcg tcgtgcatct gcacgcctcg tttttggaat acgtgctcaa tctgatcaac
    19081 cgccgctcac gtatattagc atggtttccc tacattatgt atgcgccgat tttggcgata
    19141 ctttatttta gccaatacac gatcattgac ggacgtcgcc gcattctgga tgagggcatg
    19201 agttttatac cgccttatgt tttttggctg atcgtatgtt tattggtttc cggcttttta
    19261 tcgtcacgca ttatacgtct cagccgcaac gaaaaatggc gtcaattctt tatcgtcgaa
    19321 cttatcatca cgatcgtcat ctccgtgctt ttggtgtatt tctattttta tggcggtaca
    19381 ggcaacgtgc gcctggatcg gtcgctcaaa aacatgatta tgatttcgtc gttgataccg
    19441 acgggcgcga tggtgtatct gctgtacaaa tatccttttt attcgacggt cgcaaggcgc
    19501 cggtttttat tgatcaccat ttcgggtgtt tttctcgtgg tattcattat gagctccaaa
    19561 gcgcttcgca aaatggcgga ggaaaatccc gaattaaacg tcgaatttct tgagatcgtg
    19621 ctcgtatcca tgttgtttct cgtatacgaa ccgaccaaat atcttgtacg ccgcatcgcg
    19681 gggcaatttg cgttgcatga aaaatcgtat ttccaatcgt tgattcgtaa gatcacggaa
    19741 catatcgtca gcgccgccaa tcttgccgaa ctgacaaaag ccgttcgcgc cggtatcgtg
    19801 gatgcactgc atgtcgaaga agtcgggtta tacacgttga ataaaaccgt atccggagag
    19861 cgaacgatat acagtatcgt gcatgcatac ggtgatatca aaacgtttaa tctcgaaaag
    19921 attacgcgcc atatccaaaa aggaaacggt ctgtatgatg cgcgaaagat gaacattttt
    19981 caccttcgca gcgccgaagt gccgtatcaa atttatctcg ggattgtttt ggataacgaa
    20041 ctggtaggga tcttggcgat cggaaaaaag aagagcaatg aggattttga attcgaagaa
    20101 aaagaattat tacttacagt agcgagtcag gttgcgattt ctatcgaaaa catacagctt
    20161 ctccaacgtc gtatcgaact tgaatccaaa atgtttgaag cggacaaact gacctcgctg
    20221 ggtatgctgt ccaccagtat tgcgcacgaa gtgaaaaatc cgctaagctc catcaaaagc
    20281 atcgtgcaat ccatgcacga tcagaaaaaa aaggaaaaca ccgacacgga agcgattcag
    20341 gatttggaaa ttattaacga agaaattgat cgcttgtcgt ccgttgtcaa tcagcttctg
    20401 cgttttgcac gaagcgacag ttcgggcatg gatcgaattg atattatcaa aatcatagat
    20461 gccatcctta cgatctcgcg tcaggaaacg cgggttcgca acatccgcat ttttacgcgt
    20521 tacgaacatg caccgctcgt cattcgctca cgccaaggcg acttgaaaga aatcatattt
    20581 aaccttatta tcaatgcgat ccaatccatg cccgaaggcg gacgcctgat gatccatggc
    20641 gcatacgtac aaatggatgc taactatttt atcggcggag cggaaacgga agtcggcgtt
    20701 tttgataaac tttaccccga cgatccgtcc gcaggttgga cggaatggag ttctgaagcc
    20761 gtcacggcta aaaaaataga agcgataaaa aaagatcccg gtacggaaat catacttccg
    20821 cccgaggccg gtaaaacaaa atgtttacgg attgccataa cggatacggg gcacggtatt
    20881 ccgccggaac gtttgggcga aattttcaaa ccatttttta caaccaaaag cgaaggtacc
    20941 ggtttgggtc tcgctatagt caaaaataaa atacaagcgc tcggaggtcg tctcgtttta
    21001 cgtagcgaac tccaggtagg aacgtcgttt gaagtgtata ttcccagcga aagtacatcc
    21061 cgatcatgag tgatataccg gtcgaaacca cccacgagaa taaatcgcat attcgcattc
    21121 ttgtcgttga tgatgagaag gcggcgcgct atggtatgct caaagcgctc aagacggaag
    21181 gttacaaact ttcagaagcc gacgacggat tatcggcgct caatttgatg gctaataatc
    21241 cgtttgatat cgtcatcacg gatgtatcta tggccgggat gaacggt
//
LOCUS       DAOQGF010000054        21101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1161_length_21101_cov_6.551601, whole genome shotgun sequence.
ACCESSION   DAOQGF010000054 DAOQGF010000000
VERSION     DAOQGF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1161_length_21101_cov_6.551601"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..3184
                     /locus_tag="PK937_08885"
     CDS             8..3184
                     /locus_tag="PK937_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNH32726.1"
                     /translation="MYRASAGSGKTFVLVRDYLKITLGKPEQFRHILAITFTNKAAQE
                     MKLRIIEALRAFSSDHTLSTSDHHLFEYLRNTLGFSDEQLRTAAQKLLITILHEYSDF
                     AVSTIDSFMQRILKAFATDTGLPISFSMELDSMRVAKSILERLYNAIDQNEELSDFLI
                     QWAQERSSEGKTHNLDSELLRFIPIIFEENHAAPLERIKTHELIDFVRLRNTIRHQMS
                     SITLEWQNKLKQIVDEIVSLGLTDEDFYRKTKGVLSFIKKSFENPFNIEPNQYVVAAL
                     TDRSLVHPDSSKKDILKTIEHKILQLIDQYLTVQKKITSYDLINKALPQLTLMRTFTE
                     LYDQFRDEELTIHISEVNHHINKIVRSEPVPYIYEFISSKYSHFFLDEFQDTSAMQWQ
                     NLVPLIHESLSYGTYRHNLIVGDVKQSIYRWRGGRAEILANLPYLTEKIKLELEEGDA
                     IESKFLETYKIVNLNTNYRSKEVIVNFNNRFFEFLKTSHWFKFNHYYDQCAQETASSQ
                     TGGYVKIEFLTQNDSNSELYYEAALKTKKIIQTCCEELKYSPHDIAILVRSQHEAIAI
                     SDILSEYQIPITTEESLLLRNAPKVRYLIAFLHWVHNPKNKNHLATWLTLAAQLTHRH
                     QDFFQNIFLIGHSSQSIPEIFDFAINELKLDTSIRKINEQPLHEALLTLIYASHHIIG
                     ATDQFVECFVKIALEYAQTQSLLLNEFLNYWEENNETLALRNDGLRSGVRIMTVHKSK
                     GLEFPVVIIPIADWSLSSTGKNKLWVNTDPERFFGMDTLLIPEIKKVQNSDFYEFYEN
                     EKELLQLDAINLLYVAMTRSRDHLYIMCPTPTANSDKENVRNVGEIISQFVIHESSQL
                     NTYEAGSSAYSNQTQHVKEQEKNLVYTQTETVKEKPWRSWIQLQSRFDNPSDLDTLWY
                     GDKNTRWGNLIHFVLVQMSRSEDLMKAIEHFKKISFVNEEEAHHIHTVLTRFFENKSI
                     VKLFDATAAIIRESEFFDTEGQLRRFDRMQIENDRITIIDFKTGQKKDTHVTQMQSYM
                     KPFVQIHHQKVQGALLYIDPPEIVEVHL"
     gene            3181..6039
                     /locus_tag="PK937_08890"
     CDS             3181..6039
                     /locus_tag="PK937_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNH32727.1"
                     /translation="MIAFLDDIATYLLKDSPTSLNDRCVVFQNQRAKLYFKKYIGQRA
                     QPGLILPEMLTIEELAERLSGKRITDNFDLLIELYLCHQKLNQPETFIKFYDLGLKII
                     EDFSDMDMYAVDPAKLFINLDYLKSTEFWDKNDEDLTVLQLRQKNFWKYLFTLHKEFK
                     RVLDEKGKAYAGMAYREAVDQIRSQNYTLPWSKVFFAGINALSLCEEEIIDHLLRRKQ
                     AEIFWDADVFYINQKHHEAGNFIRRYIKKWPESDKHWIGDRLSRDNKTIRITRASQNS
                     AQARAAVVALKESVKNKAGSIAVVLGDESLLIPVLEELPESLTKSTRINVSMGYPLSM
                     TTIHSFMLCVLQLHADALSNQTKRKEYTFLHQYVIQFFSLPLVRNLIKDKVYSIHQAI
                     IRQNSTKLSLEFIQSLTNLRSLKLVFNACCDISDMLKNIDSLIDEIKNELFINRMYEE
                     LEALYLWKGKWTIICDTYQRHTSLHNPDLSENLETIIRLIQDAVINVRIPFDSPAVNS
                     DLTQRIEIMGMLETRALDFEHVIILSVNEGVFPAPRSTHSLIPFTARAESGMPNYTEK
                     ESIFAYNFYRLLQRASTIDLIYDAQINQNTKGEPSRFIQQLRYELAKTSPNITIIENE
                     LPPLPVIKSSIEPLLIQKTPAIIDAITDVLIKGLSPTAINSYNRCSLQFYLSHIAKFD
                     ESDDIKSELQSDDLGNIVHHVFQHLFTPYIGKSIAAKDISSMLNDVDTAIQNALDDMG
                     NPDVHSGKNKITFAIAQQHIQRFLNNEKDRLSHDNTNRWVLSVESLMSTSIDVPIEGQ
                     LFSMRLHGKADRIDLIDDVVEIIDYKTGSAQKKHVQLPTTIADLSVKSDSDKALQLLT
                     YIILHRSHSGNKYKKYRSMIVALRGSPAEYYLPLTYDKNEIHSIETEIEKTSLEYFRS
                     IALELLNNERPFKQTDEKKNCVYCSYKNLCNRWPEHYS"
     gene            6046..7323
                     /gene="dgt"
                     /locus_tag="PK937_08895"
     CDS             6046..7323
                     /gene="dgt"
                     /locus_tag="PK937_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HNH32728.1"
                     /translation="MITNTFYNEFDIQLIDGVSGRHLYERTPFQQDRDRIIYTSSFRR
                     LQSKTQVFFSGEYDFYRTRLTHSLEVAQIGRSLCHYLKEESKELKDDFYIDSDLVEAI
                     CLAHDIGHPPFGHAGETTINKLMQPYGGFEGNAQTLRMLTETIYSAASGREGMNPTRA
                     LTDGVMKYKKLYSHSHNNNQPPKNHFLYDEQWPYVQFIFDHRDIPKDVDHNKFRSIEC
                     QIMDWADDTAYSINDLLDGISSGLITQAKIESWAAKSSFTNDENVLISNLLKAMSERQ
                     IARFCSRKIGDCIRAVNLSPIKNFMSDKTNRHAFYLSINKEKRAEIDLYGRISRELVF
                     FSPQIYQLEFKGDYILRKMFDAFEKHYLGNNNRNTMTLLPDHTEGPVRNEKDLKKRAR
                     LICDYVAGMTDGFALRTFKRLYDPDFGSIMDLV"
     gene            7375..7447
                     /locus_tag="PK937_08900"
     tRNA            7375..7447
                     /locus_tag="PK937_08900"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7408..7410,aa:Ala,seq:ggc)
     gene            complement(7507..8106)
                     /gene="can"
                     /locus_tag="PK937_08905"
     CDS             complement(7507..8106)
                     /gene="can"
                     /locus_tag="PK937_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNH32729.1"
                     /translation="MSDLKHLFQNNLHWSENIRAQKPDFFDKLSHQQAPEYLWIGCSD
                     SRVPANEIVGLLPGEMFVHRNVANVVVHTDFNCLSVIEYAVEVLKVKHIIVCGHYGCG
                     GVAAALQDSRLGLIDNWLRHIREVWKKYETELNTISDPQERHHRLCELNVVEQVANVC
                     NTTIVQKAWDRKQDLTIHGWIYGLQNGLIRDLKVSKDNV"
     gene            complement(8133..8699)
                     /locus_tag="PK937_08910"
     CDS             complement(8133..8699)
                     /locus_tag="PK937_08910"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH32730.1"
                     /translation="MNHLIEIKKSLKRASTVEAKFATLKAVPTAKGVYGVRMPVLNNL
                     AKTYKTCDFKLINELWKSGKFEERMLSAKIIGFKAKKEPVKTLSTIRKFSASVEDWAI
                     CDCLSSQSIRSIALSEQKRIFQLSELLITSKKIWQRRMALVLLEYYTRFPQFHPYIHN
                     AIASLENEKEYYVSKAIHWLKRNMLKKR"
     gene            complement(8739..10445)
                     /gene="recN"
                     /locus_tag="PK937_08915"
     CDS             complement(8739..10445)
                     /gene="recN"
                     /locus_tag="PK937_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNH32731.1"
                     /translation="MITSLTIKNFALIEDAEIIFGKGLNIITGETGAGKSILLGAISM
                     ILGERASTDHIRNGADKAIIEGIFHTKPNRFLKAIFTENDLGWSDELVLRREISTRGQ
                     NRCFVNDNLITTGLLKSIGDYLVDLHGQHEHQSLIRQEFHIEFLDAFANNAENVQQLT
                     ELYKSVTALQRRIHEIETNKQSIIDKKEYYSFQLKEIEEVNPQPGEDESLIQEEKLAS
                     NSEKIYELSHSAYQDLYESDGAIIERLEQVKSKLNQLSSFDERLIDYQKDFDSAVIAI
                     AEIARVLGEYNHKIVFDPERLDLMRARLMNLQKLKRKYGSIEDIIRAKEEYRGYVSLS
                     ENYDEQLLALKKELNKSIESYGNCAHAVSAARIKAAEKLDKRVVQELVKLGIEKAQFK
                     TMITQIEAPNGWFISKDKSIQATAQGIDHVEFFISANAGESLKPLVKVASGGEISRIM
                     LSLKTALAQSDKIPTMIFDEIDVGISGRIAQAVGIALSELSESHQIISITHLPQIAAL
                     AQTHFSVQKQTQGHHTTTTIRQLKENERETEIAKLLGGATITDAAIKNARDLMRQKVG
                     FL"
     gene            complement(10453..10923)
                     /gene="ribH"
                     /locus_tag="PK937_08920"
     CDS             complement(10453..10923)
                     /gene="ribH"
                     /locus_tag="PK937_08920"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNH32732.1"
                     /translation="MPTSSLKSPNLSFGIIKSAFNQNVVNLLFEGLARGFQDAGIDQK
                     YIDVYDAPGAMELPVSAKHLCETGKYSAVIALGCVIRGNTPHFEYVSAETTRGLSQVS
                     LTTGIPVIFGVLTVNTLEDAMDRVKNDKSNKGYEAAMAAVEMAKLILRIKSSRQ"
     gene            complement(10923..11780)
                     /locus_tag="PK937_08925"
     CDS             complement(10923..11780)
                     /locus_tag="PK937_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32733.1"
                     /translation="MLKYKFYICCAHIIALGLAAQTYETQSSPIVIKKQNEMVVSKPS
                     TLKAIGLSILMPGAGELYLKKGKRSYWAAGLFMVAETATWITYFSYRNAGLKKEKEYI
                     RYADKNWNVDEYLRFLETTLQLSDGYLGRKSSGIDKDKLEVAENEWGSLSGISVHHLY
                     KSGRQQYYEMIYKYPEQFGQGWSDANWDLVDIPNRSTGYTPYNLTDRMKYYRSMRVKS
                     NAWLENARNATGYMLANRVLSVLDIVWITKKYKASNSTESVQMGIRIKPVKDLNLRLK
                     AYPSLEVTF"
     gene            complement(11780..13936)
                     /locus_tag="PK937_08930"
     CDS             complement(11780..13936)
                     /locus_tag="PK937_08930"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNH32734.1"
                     /translation="MKKQIFILAGMLCLSNFSISSKLMAQSGIDSIDYWKTQAVENIR
                     EFERLPVTVKAVSLIFGDKISRAKKNIIRALVVKPEDVESKYIYAIVQRYESQFDDAE
                     KNIKSIIDVNDRFSAYGFPNVWIQLGYIYKSKMENDLAIDAFKQGALIDLNDTWPLIE
                     LSFVFSEIGKDVEACEAFYGGLNEINDPQKIEKLFVDCRDIASEKEIKIWEDSLKANK
                     EKLEFLKIFWLKRDPNPLDPVNQRLLEHYKRLIFARSNFSKSLSPWYDDRGQTYIRLG
                     KPDIRYIGRTAEAVKENESWVYDRIKSGLYFDFIELNGSYKLGSLFDALNMSSRASSM
                     VEIFNERSHINPYYQRMADRIKTQTDVVHDRAVEAFNNTVSTKPGEGSESHLNAVDRS
                     SFASTFLNTNNYSQDYLANIEYSKKQNFIFDVGAPHLPMNCNFASFRSVNKKDSRLEF
                     YYTVPFKVLNFAPSITQVDKFNTSIKLHMKLYDLKYNEVKSVERDFDISAFGKETTSH
                     FFLDQFDADITPGKYIAAVEMRNNEKDRVGIYQFVLNVRDYNSDSLLVSDIEIAQYVD
                     QTLTKDKYLKPQSNLKVVPNPAAGLVNTKPLTIYYEIYNLAKNADNRTSYQVSYSIKM
                     LESNQSFLSSVAGVFTGKKEASTSSVTVKEGKSVNEKEYIAFDIADLPTGVAALEVRV
                     KDLNSGKEAVSATNITITSAREEQNNASSEESVKEK"
     gene            14199..14693
                     /locus_tag="PK937_08935"
     CDS             14199..14693
                     /locus_tag="PK937_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32735.1"
                     /translation="MKTEKTQKPNLQQLTSTHQEIDLISNSKKPTVLLVEDDDIAGST
                     IAMMVETLHCSVIRVENGLDALSIVGSMKIDLVITDLCLPVMSGTEIIEHIRKINKTV
                     PIFIVTGYLHTATETEMISVSDKKSCGVIEILMKPIRMKNLLDKIKEHTLIENAPKAA
                     LMKI"
     gene            complement(14898..15536)
                     /locus_tag="PK937_08940"
     CDS             complement(14898..15536)
                     /locus_tag="PK937_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNH32736.1"
                     /translation="MKKLGLIGLGIFAVWLSSCSVAKQIDMAKTFAKCDFSVQNIEHM
                     QLADIKIQNVKSAKDLSLKKAAQLTDALTKKTLPLTFTINLSVENPNEKPAGMNRLEW
                     IVLIDNNEIVRGALDQIVEILPDSDATVPLNIEMDLKKSFSNESAESLVKLAFNLAGE
                     GDKPTRITVKVKPTINVAGLNIEYPGYIDVHTKINSEQGKQLRKKAAKKAKK"
     gene            complement(15553..19965)
                     /locus_tag="PK937_08945"
     CDS             complement(15553..19965)
                     /locus_tag="PK937_08945"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH32737.1"
                     /translation="MKLIRIPFLSLIVIWGCSQVQLSTQNDKARHYYLEGLKLEKKNS
                     DAIHAALENYKNAIREDATFYEAYVTIASLYEKQGSYRKAALYFEKALALRHEPGRAA
                     LPYARTLYNLNRFEESYELAKYYNENYPSDAQGLSFLADAARALNKPGIESDLLRLTK
                     MDSANAYAWSSLAQYYYDQGDFAMAIPIYDRMLREKLHVTTDMRYEYALCQLHVGLWN
                     DALPHLEQVIREGINDKYPPHLAQAVRLIISGTFKEEALNLFLKAMIAFESVQNGHTD
                     RQEGYGLAKQSLTKSLEIEPNFFLSRHLAARLLYVMEEYDEAFINYEWLTKNNMADSD
                     DFANMGFLLFKKNQMKEAQAYYEKSLSIKPDQPSVKSSLETIRRINEGKISPQAYVYF
                     DKALAHTSGFDSTSYYLRLAIAADSLYPEAYLQLGLLQYRNGQYKAAEPTLLHGLQLA
                     YTDKLKKNFHYNLALVYSRLDWHDKSIQQFLQCLRIDTADGDALYYLSKVYADKSDYE
                     NAVSIYETLSRRVPEYFVPDENDLQQAGIGQFSQSIGPSRFYFDDSLHIGQTNTYRLK
                     IKSKNDALLGADANGDLSREIIITFQEQVLDITSYGVVEFALDILEINGYAIAAKEKA
                     IQGQRFYLRISDIYGVTNIYGLMEEDPAGLSRLVIAVMEDLHGNFTRRVISEGEIWRS
                     GQNIFKLGNLDAVTELDDINNQIAYGKKYYGVLGSYDAARYGETGYVTIQNKGESEFE
                     FDIQKRLIRKLKNVFVTKEFRDATAKLTQQDASYAVDLIHTTIENLEKPKKVVLENIP
                     YVRQHGPQCAAASLSMVLSYYKQSIDQDEIYEKIKSDYAGAQSNDIIHYPRSLGKYKS
                     FGYVGTLDDLKRRIDQGIPVLVFLSPFGFGHVVVVIGYDETKHQIIMHDPTVANNHAI
                     PYDDFLREWQQSGNECAIVLPLETPVVVTEEPLNSYQAVEKKWEGDKASGVRNFDKAI
                     QLYRESLRLKPNYEGAMEGMMLLHLQKDEFDKASVIMDTLLQMRPNSIDLILRRAGLL
                     LSQYDYDKVLQITRRAKQIDESNITNYLYSASALFSQKKYNEAMNELKQAIRINPLIS
                     QPRTFYASILAETGDFEKAHEQMNFALRYEPENIGNYLSVAGLYQTEINVNFLTAQEK
                     ADRIKKAIEALDIVKQANPSLPNLDQIYADIYTAVDDFKRGDSLMFENIRKYPEENGA
                     YNNIAWRYAVSGIKLDVAEKYSQKSIELSQRNPYYFDTIGWIHLKMAVQVRKIYPDSA
                     ESFMKKAESEFLATIEYDMYSDFAYRHLGVLYAVWGKLAQATTEFEKVAAMMPDKARI
                     YAEIGQDCEELGLYEISMNYYRKAIDIRPNFAYAKYRMAYILAIKMNDYTKAESYILE
                     AIQVDSVNFLYTGMQGIIAFLKQDIPVAIRILDKVCFNQSAFTDEEAAINNYYFGMAL
                     LKTNQIAQAKTQFMEYLRRSPDGEYSEIVKKRIK"
     gene            complement(20054..20446)
                     /locus_tag="PK937_08950"
     CDS             complement(20054..20446)
                     /locus_tag="PK937_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNH32738.1"
                     /translation="MILILCFCCEFNAYARSGGLQVFCLAPAVYSKNRSESEISEKIQ
                     QKINYYSLCYSQNFDNKTATSFKVRFRFVIKNDGSIVDITTVFSGTKNQAFIECLTNM
                     LKQIYFQKIGTEAGDTIVEQSILFKIQS"
     gene            complement(20465..21025)
                     /gene="amrA"
                     /locus_tag="PK937_08955"
     CDS             complement(20465..21025)
                     /gene="amrA"
                     /locus_tag="PK937_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNH32739.1"
                     /translation="MNLDNETKKTLIHIARMTINAAVKRTKEPNYEKDVATFPILKEK
                     AGAFVTIEKRGHLRGCIGYIEAREPLYKTVIMAARSAALNDDRFGSLQLDELNEIELE
                     ISVLSTFSRVEDINDIMVGTHGLMVESGFYHGLLLPQVATEYQWDRITFIEQTCHKAG
                     LNKHAWKEKTTVIKSFTAIVFNERDI"
BASE COUNT         6443 a         4249 c         3752 g         6657 t
ORIGIN      
        1 ccttacaatc tatcgcgctt cggcaggatc cggcaaaaca ttcgttttgg tgcgcgatta
       61 tcttaaaatc actctgggta aacctgagca atttcggcat attctcgcta ttacgtttac
      121 taataaagcc gcccaagaaa tgaaacttcg gattattgag gcgttgcgag ctttttcttc
      181 cgaccataca ttatcaacat cagaccatca tttatttgaa tatctacgta atactttagg
      241 tttttcagat gaacaattga gaactgccgc tcaaaaactt ttgataacca ttctgcatga
      301 atatagcgat tttgctgttt cgaccattga cagttttatg cagcgtatat tgaaggcctt
      361 tgctactgat acagggcttc cgatcagttt ttcgatggag ctcgacagta tgagggttgc
      421 taagtctatt ttagagcgat tatataatgc gattgaccag aacgaagaat tatccgattt
      481 cctcatccaa tgggcgcaag aacggtccag cgaaggtaaa acacataatt tagacagcga
      541 attattgcga tttatcccca taatttttga agaaaatcat gctgctcctt tagaacgaat
      601 caaaacgcac gaattgattg atttcgtgcg attacgcaat acaatacgcc atcaaatgtc
      661 aagcattact ctggaatggc agaataaact aaaacaaata gttgatgaaa ttgtgtcgtt
      721 aggattaact gatgaagatt tttatagaaa aactaaaggc gtactttcgt tcattaaaaa
      781 aagctttgaa aatccattta acatagagcc taatcaatac gtcgttgcgg ccttaaccga
      841 tcgttcgttg gtacatcctg attcttccaa aaaagacatt ttaaaaacta ttgaacataa
      901 aatattacaa ttaattgacc agtacttgac ggttcaaaaa aaaatcactt cttatgattt
      961 gataaataaa gcgttaccgc aactaacttt aatgcggaca ttcaccgaac tttacgatca
     1021 attccgtgat gaagaactta cgatacatat ttcagaagta aatcatcata taaacaaaat
     1081 cgttcgtagt gagccggttc cgtacatata tgaatttatt tcgtctaaat atagtcactt
     1141 ttttttggac gaatttcaag atacctctgc tatgcaatgg caaaacttag tgccgttgat
     1201 tcatgaaagc ctctcttacg gcacgtatcg acacaacttg atcgtcggtg acgtcaaaca
     1261 atccatctac cgctggcgtg gtggccgcgc tgagatatta gcaaatctac cctatcttac
     1321 cgaaaaaatt aaactcgaac ttgaagaagg cgacgccatt gagtctaaat ttttagaaac
     1381 gtataaaatc gtcaacctta atacaaatta tcgaagtaag gaagtaatag tcaattttaa
     1441 caatcgtttt tttgaattct taaaaacatc gcactggttc aaattcaatc attactatga
     1501 tcaatgtgcg caggaaaccg catcttcgca aaccgggggt tatgtcaaaa ttgaattttt
     1561 gactcaaaat gacagtaata gcgaactgta ctacgaagcc gctcttaaaa ctaaaaaaat
     1621 aatacaaacg tgttgtgaag agttaaaata ttctcctcac gacattgcca ttctcgttcg
     1681 atctcagcac gaagctattg cgatctctga tattttatca gaataccaaa ttcccataac
     1741 aactgaagaa tccctattac ttagaaacgc tcccaaagtt cgatacttaa ttgcattttt
     1801 gcattgggtc cacaatccaa aaaacaaaaa ccatcttgcc acttggctga ctttggcagc
     1861 gcagttaaca catagacatc aagacttttt tcaaaacata tttttaatcg ggcactcttc
     1921 ccaatctatt cctgaaattt ttgattttgc tatcaatgaa ttaaaattgg atacatcaat
     1981 aaggaaaatc aacgaacaac cattacacga ggcgctgcta accctcatat atgcaagtca
     2041 tcacatcatt ggtgcaacag accaatttgt cgaatgtttt gtaaaaattg ctttagaata
     2101 cgcacaaacg cagtccttgt tattaaatga attcttgaat tattgggagg aaaacaacga
     2161 aacacttgcg ttacgaaatg acggtttgcg tagcggtgtt cgcatcatga cggtccataa
     2221 atcaaaagga ttagaatttc cggtagttat tattcccatt gctgactggt cacttagttc
     2281 gaccggaaaa aacaaactat gggttaatac tgatcccgag cgcttttttg gtatggatac
     2341 actgctgatt ccggaaataa aaaaagttca aaattctgat ttttatgaat tttatgaaaa
     2401 tgaaaaagaa ttattgcaac tggatgcaat aaaccttttg tatgtcgcga tgacacgaag
     2461 ccgtgatcat ctctatatca tgtgccctac tccaaccgca aattcagaca aagaaaacgt
     2521 taggaatgtt ggtgaaatta taagccaatt tgttattcat gaatcaagtc agttaaacac
     2581 atatgaagca ggtagctccg cctattcgaa tcaaacgcag cacgtaaaag aacaggagaa
     2641 gaacctcgta tatacacaaa ccgaaaccgt taaagaaaaa ccatggcgtt cttggatcca
     2701 attacaaagc cgatttgaca atccgagcga tttagatacg ttatggtatg gcgataaaaa
     2761 tacacgctgg ggtaacctca ttcatttcgt tcttgtacaa atgtccagat ctgaagatct
     2821 gatgaaggca attgaacact tcaaaaaaat aagcttcgta aatgaggaag aagcgcatca
     2881 tatccacact gttcttacaa ggttttttga aaataaatct attgtaaagc tgtttgacgc
     2941 tacggcggcg attattcgtg aatccgaatt ttttgatact gaaggacaat tacgccgatt
     3001 tgatcgtatg caaatcgaaa atgaccgtat cacgattatt gattttaaaa cagggcaaaa
     3061 aaaagatacg catgtaacac aaatgcaatc atacatgaaa ccgtttgttc aaattcatca
     3121 ccaaaaagta caaggtgcgc tgctgtatat agatccaccc gagatcgttg aggtgcatct
     3181 atgatcgcat ttcttgatga tatagcaacc tatttactca aagattcccc aacgtcatta
     3241 aatgatcgtt gtgttgtttt tcaaaaccaa cgcgcaaaat tgtattttaa aaaatatatc
     3301 ggtcaaagag ctcaacccgg tttgatatta cctgaaatgc taaccatcga agaattagcc
     3361 gagcgtttat cgggtaaacg cattacggat aattttgatc ttttgattga gttgtattta
     3421 tgccaccaaa aattgaacca acccgaaact tttataaaat tttatgatct gggtttaaaa
     3481 attatagaag attttagcga tatggatatg tatgccgttg atccggcaaa gttatttata
     3541 aatcttgatt atctgaagtc aactgaattt tgggataaaa acgatgaaga tttaaccgtt
     3601 ttacaactac gacaaaaaaa cttttggaaa tatctgttca ccctgcataa agaatttaaa
     3661 cgagttcttg acgaaaaagg gaaagcttac gccggtatgg cttatcgcga agccgtcgat
     3721 caaattcgca gtcaaaacta cacgttacct tggtcaaaag ttttttttgc aggaattaat
     3781 gcgcttagtt tatgtgagga agaaataatt gatcatttac taagacgaaa acaggctgaa
     3841 attttttggg atgctgatgt tttttacatt aatcagaagc accatgaagc cggtaacttc
     3901 attcgccgtt atatcaaaaa atggcctgaa tcagataaac attggatcgg agatcgtcta
     3961 tcacgcgata ataaaacaat acggatcaca cgcgcatctc aaaatagtgc acaagctcgc
     4021 gcagcggttg ttgcactgaa agaaagtgta aaaaacaaag cgggatccat tgccgtcgta
     4081 ttaggtgatg aatctctgtt aatacctgtt ctggaagaat tgccggaaag cttaactaaa
     4141 tcaacacgca tcaatgtaag tatgggttat cccctttcaa tgacgacaat tcatagcttc
     4201 atgttgtgtg tactacaact gcatgccgat gcattatcaa atcaaacaaa acgaaaagag
     4261 tatacgtttt tacaccaata cgtaattcaa tttttttcat taccattagt tcgcaattta
     4321 attaaagata aggtttactc tatacaccag gccatcataa gacaaaattc aactaaatta
     4381 tcacttgaat ttattcagtc gcttacaaat ctgcgatctt taaagcttgt atttaatgct
     4441 tgttgcgaca ttagcgatat gttgaaaaac attgactctt tgattgatga aattaaaaat
     4501 gaattgttta taaatcgaat gtatgaagag cttgaggcgc tgtatctatg gaagggaaag
     4561 tggacaataa tttgtgacac atatcaacgc catacgagct tacataatcc cgatttgagt
     4621 gagaatttag aaacaatcat acgcctaatt caggacgctg tgattaatgt tcgcatccca
     4681 ttcgattccc ctgccgtaaa ttcggatttg actcaacgta ttgaaatcat gggtatgctt
     4741 gaaaccagag cattggattt tgaacatgtc attatcttat ccgtcaatga aggcgtattt
     4801 cctgcgccgc gttcgactca ttctttgata ccatttacag ctcgtgccga atcaggaatg
     4861 ccaaactata ctgaaaaaga gtccatattt gcttataact tttacaggct tctacaaaga
     4921 gcttcaacta tagatttgat ttatgatgca caaatcaacc aaaatacaaa aggcgaacct
     4981 agtcgattta tacaacagct tcgatatgag ttagccaaaa catcgccaaa cattactatt
     5041 atagaaaatg aattaccccc cctgccagtt ataaaatcta gcatcgaacc cttgttaata
     5101 caaaaaacac cggccattat cgatgccatc acggatgttt taatcaaagg gttatctcca
     5161 acggccatca actcttacaa tcgatgttca ttgcagtttt atttatcgca cattgctaaa
     5221 tttgacgaat ccgacgacat taaatctgaa cttcaaagtg atgaccttgg aaatatcgtc
     5281 catcacgtat ttcagcactt gttcactccg tatatcggaa aatctattgc cgccaaagac
     5341 attagttcaa tgctgaatga tgtggataca gcgatccaaa atgcattgga tgatatgggc
     5401 aatccggatg tccacagtgg aaaaaataaa ataacattcg ctattgctca acagcacatt
     5461 caacgttttt tgaacaatga aaaagaccgt ctttcacacg ataatacaaa tcgatgggtc
     5521 ctatctgtcg aatcacttat gtccacttca atagatgtcc caattgaagg tcaactattc
     5581 tcaatgcgcc tgcatggaaa agcggatcgt attgacctta ttgacgatgt tgttgaaatc
     5641 atagactata aaactggttc agcccagaaa aaacacgtcc aattaccaac aaccattgct
     5701 gatctttcag ttaaaagtga ttctgataaa gcgttgcaac ttctaacata tatcatacta
     5761 caccgttcac attccgggaa taaatataaa aaataccgaa gcatgatcgt tgctttgcgt
     5821 ggatcacctg cagaatatta tttgccgcta acttatgata aaaatgagat acactcgatt
     5881 gagactgaaa ttgaaaaaac atctttagaa tattttcgaa gtattgctct agagttactc
     5941 aataacgaac gaccatttaa acaaacagat gaaaaaaaga attgtgtata ttgctcctat
     6001 aaaaatctat gtaaccgatg gccggaacat tattcatgag ggaaaatgat aactaataca
     6061 ttttacaatg aatttgacat tcaattaatt gacggcgtaa gtggacgcca tctttacgag
     6121 agaactcctt ttcaacaaga tcgtgatcgt attatttaca catcctcttt tcgaagattg
     6181 caatccaaaa cacaagtatt tttttctggc gagtatgatt tctatcgaac acgactaact
     6241 cattctttag aagtggcgca aatcggccga tcattgtgcc attatctgaa agaagaatcc
     6301 aaagaactta aggatgattt ttacattgac tctgatcttg tcgaagcgat atgcctcgct
     6361 catgatatag gccacccgcc ttttgggcat gccggagaaa cgacaattaa taaattgatg
     6421 caaccgtatg gagggtttga gggtaacgca caaacgctac gaatgttaac cgaaacaatt
     6481 tatagcgccg cttcaggtcg cgaaggaatg aatcccacaa gggctttgac agatggtgtg
     6541 atgaagtata aaaagcttta ctcgcatagt cataataaca atcaaccgcc caaaaatcat
     6601 tttttgtatg atgaacaatg gccatatgtt caattcattt ttgatcaccg cgatatacct
     6661 aaagatgtgg atcataataa gtttcgtagc atagaatgcc aaatcatgga ttgggcggat
     6721 gacacagctt attcgattaa cgatttattg gacggtatta gctcggggct gataacacaa
     6781 gccaaaattg agtcttgggc cgctaaatca tcatttacta atgatgaaaa cgttttgatt
     6841 tcgaatctgt tgaaagctat gtctgagcgt caaattgcac gtttttgcag tcgtaaaatt
     6901 ggtgactgta tacgcgccgt taatttgtcg ccgataaaaa attttatgtc cgataaaaca
     6961 aatcgccatg cgttttacct ttctataaat aaagaaaaac gtgcagaaat tgatttatac
     7021 ggtcgtattt caagagagct tgtatttttt tcaccgcaaa tctatcaact agaatttaag
     7081 ggggattata ttttaagaaa aatgtttgat gctttcgaaa agcactacct cggaaacaat
     7141 aaccgaaata caatgacttt gttgccggac catacagaag gaccggttcg taatgaaaaa
     7201 gatttaaaga aacgtgctcg gttgatatgt gattatgtcg cgggtatgac ggacggattc
     7261 gctttgcgaa ctttcaaaag attatacgat ccggatttcg gctcaattat ggatttggta
     7321 taaaaaaaac ttgctttttc atacttgctc gtatagatta cgcgcacctt ttacggggac
     7381 atagctcagt tgggagagcg caaggctggc agtcttgagg tcaggggttc gacccccctt
     7441 gtctccacga aaaaaccttc tctgtagaga aggttttttt attatgcaca tcatttttac
     7501 ttcgttctat acattatcct tagatacctt caggtcccta atcaatccat tttgtaaacc
     7561 gtaaatccag ccgtgtatcg taagatcctg tttacgatcc catgcttttt gtacgatcgt
     7621 cgtattacat acatttgcaa cctgctcgac gacattcaat tcacataaac gatgatgtct
     7681 ttcttgcgga tccgatatgg tattcaactc ggtttcgtat tttttccaaa cctcacgaat
     7741 atgccgtaac caattatcga tcagtcccaa tcgactatcc tgaagggctg ccgccactcc
     7801 accacatcca tagtgtccgc acacgatgat atgttttact ttaagcactt cgacggcgta
     7861 ttcgattacc gataaacagt tgaaatcagt atgcaccacc acattagcca cgttacgatg
     7921 aacaaacatt tcacccggca aaagtcctac aatttcgttg gccggaactc tgctgtcgga
     7981 acatccaatc cataaatatt ccggcgcttg ttgatgggaa agcttatcaa aaaagtccgg
     8041 cttttgtgct cgaatgtttt cagaccaatg aagattattt tggaacagat gctttaaatc
     8101 agacatagtc aatagacctg taggtttgtt ggttaacgtt tttttaacat attacgctta
     8161 agccaatgga tggctttaga aacgtaatat tctttctcat tttcaagact ggcaatagcg
     8221 ttgtgaatat acggatgaaa ttgcggaaaa cgcgtgtaat attccaataa aactagagcc
     8281 attcgtcttt gccatatttt tttggatgtg atcagcagct ctgaaagctg gaaaattctt
     8341 ttttgttccg ataaagcaat agatcgaata ctttgggagc tcaaacaatc gcagatcgcc
     8401 caatcttcaa ccgatgcgga gaattttcgg attgtagaca atgtcttcac cggttctttt
     8461 ttagctttga atccgataat tttagcggat aacatgcgtt cttcaaattt tccggatttc
     8521 cataactcat taatcagttt aaaatcgcaa gttttatatg ttttagccag attattgagt
     8581 accggcatac gaacgccgta aacgccttta gctgtaggaa ccgctttgag agtagcgaac
     8641 ttagcttcaa ctgttgaggc cctttttaag cttttcttta tttcaataag atgattcata
     8701 caataaccat cggacgcaat ttggaagtaa aaacacggct atagaaaacc gaccttttga
     8761 cgcatgagat cccgtgcgtt tttaatggcc gcatcagtga tggttgctcc gcctagaagt
     8821 ttggcaattt ctgtttctct ttcattttcc tttagttgcc gaatcgttgt tgttgtatga
     8881 tgcccctgcg tttgcttttg aacgctaaaa tgtgtttggg ctaatgcagc tatttgaggt
     8941 aagtgcgtga tgctaatgat ttgatgagac tctgacagct cacttaatgc aattccaacc
     9001 gcttgtgcaa tacgcccgga tatgccgaca tcaatttcgt caaatatcat ggtgggaatt
     9061 ttatctgatt gcgctaaggc tgtttttaaa ctaagcataa tacgcgaaat ttcgccgccg
     9121 gaagcaactt tgacgagcgg ttttagtgat tccccggcat tagccgaaat gaaaaattct
     9181 acatgatcga taccttgcgc cgtggcttga atggatttat ctttcgaaat aaaccatcca
     9241 ttcggtgcct cgatctgcgt gatcatggtt ttaaattgtg ctttttctat gccgagctta
     9301 accaactctt gcacaacacg tttatcaagc ttttcagcgg ctttgattcg tgccgcagaa
     9361 accgcatgtg cgcaattacc gtagctttca atagatttat ttagttcttt ttttaaagcc
     9421 agaagctgtt catcataatt ttccgataat gaaacatagc ctcgatactc ttctttggct
     9481 cggattatgt cttcaataga accgtattta cgtttcaatt tttgtaaatt catcaatcgc
     9541 gctcgcatga gatctaaacg ttcaggatca aatacgattt tatgattata ttcacccaaa
     9601 acgcgggcaa tttccgcgat agcaattacg gcactatcga aatctttctg ataatcaatc
     9661 agcctctcat caaacgaact aagttgatta agttttgatt ttacttgttc caaacgttca
     9721 ataatggccc catcggattc gtataaatct tggtacgcgc tgtgactcaa ttcatatatt
     9781 ttctccgaat tcgaagctaa tttttcttct tgtattaatg attcatcctc gcccggttga
     9841 gggttgactt cttcgatttc tttaagttgg aaactatagt attctttttt atcaattatg
     9901 ctctgtttat tggtttcaat ttcatgaatg cgacgttgga gtgccgtaac tgatttatat
     9961 aattcagtca gttgttgtac attttcagcg ttgttggcaa atgcatccaa aaattcgata
    10021 tggaattctt ggcgaatgag cgattgatgt tcgtgttggc catgcagatc cactagataa
    10081 tcaccgatgg atttaagcaa tccagtagtg ataagattgt catttacaaa acaacgattt
    10141 tgtccgcgtg tcgaaatttc gcggcgcaat acaagttcat cgctccatcc caggtcattc
    10201 tcagtgaaga ttgcttttaa aaagcgatta ggtttagtgt gaaagatacc ttcaattatc
    10261 gctttatcag cgccgttacg aatatgatcc gtcgaagccc tttcgcctaa tatcatcgaa
    10321 attgcaccca atagaatgga ctttcctgca cccgtttcac cggtaataat attcaatccc
    10381 ttaccaaaga taatttcagc atcctcgata agcgcgaaat tcttaattgt tagcgaagtg
    10441 atcataaagg cattattggc gggatgattt gatccttaga attaacttgg ccatttcgac
    10501 ggcggccatc gcagcttcat accctttatt cgatttatca tttttgacgc gatccattgc
    10561 atcttctaag gtattaaccg ttaatacacc gaaaataacg ggaatgccgg ttgttagact
    10621 tacttgtgat agtccgcgcg tagtttcggc tgaaacgtat tcaaaatgcg gtgtattccc
    10681 ccttattaca caacctaagg caatgactgc ggaatattta cctgtttcgc ataaatgttt
    10741 tgccgagaca gggagttcca tagcgcccgg ggcatcataa acatcaatgt atttttgatc
    10801 aatacccgca tcttgaaacc ctcgcgccaa accttcaaac aataaattta ctacattttg
    10861 attgaatgcg ctttttatga tcccaaaaga aagattagga gactttaagg aagaagtagg
    10921 cattagaagg ttacctcaag cgatggatat gcttttaacc ttaaattaag atccttgacc
    10981 ggcttgatac gaatacccat ttgaacagat tctgtagaat tagaagcctt gtacttctta
    11041 gtaatccaca caatatctaa tacactaagt accctattcg caagcatata acccgttgca
    11101 tttctagcgt tttctaacca agcattgctt tttacacgca tgcttcgata atatttcata
    11161 cgatccgtta aattataagg cgtatatcca gtagaacgat tggggatatc aaccaaatcc
    11221 caattcgcat cactccaccc ttgtccaaac tgctcaggat acttatagat catttcataa
    11281 tattgttggc gtccgctttt gtataaatga tgcactgata taccgctaag gcttccccat
    11341 tcgttttcgg caacttcaag tttatcttta tctatacctg atgatttgcg acctaaatag
    11401 ccatctgaaa gctgcaaagt tgtttctaaa aaacgaaggt actcatctac attccaattt
    11461 ttatcagcat atctaatgta ttctttttct tttttaagcc ctgcattgcg gtaagaaaaa
    11521 taagttatcc atgttgcggt ttctgcaacc ataaagagac ctgctgccca ataacttctt
    11581 tttcctttct tcaaatacag ttcgccggct ccgggcatta agatggataa acctattgct
    11641 ttcaatgtag atggttttga aacaaccatt tcattttgtt ttttaatgac tatcggcgat
    11701 gattgcgttt cataggtttg agcagcaagg cccagtgcaa taatatgagc gcaacaaata
    11761 tagaatttgt acttcaacat tatttttctt taacactttc ctcagaagat gcattattct
    11821 gctcctcacg ggcgcttgta attgtgatgt tagtcgcact taccgcttct ttaccggagt
    11881 ttagatcttt gacacgaact tcaagagcag cgactccggt cggcagatcc gcgatatcaa
    11941 acgctatata ttctttttca ttcacggact taccttcttt gacagtaaca gaagacgtcg
    12001 atgcttcttt ctttccggta aaaacaccgg ctacggagct caaaaaactt tgattgctct
    12061 caagcatttt aattgagtac gatacctgat acgatgtcct attatctgcg ttcttggcta
    12121 aattatagat ttcatagtag atggttaatg gcttggtatt aactaatccg gcagccgggt
    12181 tgggcacaac tttaagattc gactgtggtt taagatattt gtctttagtt aaagtttggt
    12241 caacatattg agcgatttct atatcgctga ccaataatga gtccgaatta taatcccgaa
    12301 cattaagaac gaattgatat ataccgacgc gatccttttc gttatttctc atttcaacgg
    12361 ctgcaatata ttttcccgga gtgatatccg catcaaactg atctaagaaa aagtgcgatg
    12421 ttgtttcttt tccaaatgcc gatatgtcaa aatctctctc aacagatttc acttcattat
    12481 atttcaaatc atataatttc atatgaagct taattgatgt attgaacttg tccacttgcg
    12541 taatgcttgg cgcaaaattg agcactttaa acggaactgt ataataaaac tccaatcttg
    12601 aatctttttt attaacactc cggaacgatg caaaattgca attcatgggc aaatgcggtg
    12661 caccgacatc aaatatgaaa ttttgttttt ttgagtattc tatattggct aagtaatctt
    12721 gagagtaatt gttagtattt agaaaggttg atgcgaaaga actgcgatct accgcattca
    12781 aatggctttc agaaccttct cctggtttag ttgaaaccgt attgttaaac gcctcaactg
    12841 ctctatcatg aacaacgtca gtttgtgttt tgatgcgatc agccatgcgt tgataatatg
    12901 gatttatgtg tgatctttcg ttgaaaatct caaccatcga gctagctcgg cttgacatat
    12961 tcaaagcatc aaacaatgat ccaagtttat aacttccatt taattctata aaatcgaaat
    13021 agagccccga ttttattcta tcatacaccc aagattcatt ttcctttaca gcttctgcag
    13081 tacgtccaat atatcttata tccggttttc ccaaacggat ataagtctga ccgcgatcat
    13141 cataccatgg actgagcgac ttactgaaat tagaacgagc gaaaataagc cttttgtaat
    13201 gctctagcag cctctgattt actggatcca acgggttggg atctcttttt aaccaaaaaa
    13261 tttttaaaaa ctctagtttt tccttgttgg cttttaatga atcctcccat atcttaattt
    13321 ctttctctga agcaatatct ctgcaatcaa caaataattt ttcgattttt tgcggatcat
    13381 taatttcgtt tagtccaccg taaaacgctt cacacgcttc gacatcttta ccgatttcag
    13441 aaaaaacaaa tgaaagctca ataagcggcc atgtatcgtt caaatctatg agtgcacctt
    13501 gtttaaaggc atctatagca aggtcatttt ccattttact tttataaata taaccaagtt
    13561 gaatccatac attcggaaat ccataggcag aaaaccgatc attaacgtca ataattgact
    13621 taatattttt ttcggcatca tcaaactggg attcatacct ttgtacgata gcataaatat
    13681 atttggattc aacatcttct ggtttaacca caagtgcgcg aattatgttt tttttagctc
    13741 ttgatatttt atctccaaat atcaaagaaa cagcctttac tgtgacaggc aatctttcaa
    13801 attctcgaat attttccaca gcttgagttt tccaataatc aattgaatct atccccgact
    13861 gcgccattag ctttgagcta attgaaaaat ttgataagca taacatccca gctaaaataa
    13921 agatttgttt tttcatgtct tgcctttctt taatcatttt aaggtttaaa tataagcttt
    13981 cattttttta attcaaaata aatcaaactt tcaaaattta ttattgttga cgtcaattat
    14041 ttgacgccta attattaatt tacttctatc aataagaata aaaaatataa gtaattaatt
    14101 attaatattt taaaataaag catcgcatca aaaatattgg cattatttat gcagttaaat
    14161 aatatggttc tcaaaagcta atcaatacta ataaaatttt gaaaacagaa aaaacacaaa
    14221 agcctaattt acagcaactc acatctacac atcaagagat cgatctgatt tcgaattcaa
    14281 aaaagccaac tgttttatta gtggaagatg atgacattgc cggttcgact atagctatga
    14341 tggttgagac attacattgt agtgtgatac gggttgaaaa tgggcttgat gcgttaagta
    14401 ttgtcggctc catgaagata gatttagtta ttactgattt gtgtttaccc gtcatgtcgg
    14461 gaacagaaat tattgaacac atccgaaaaa taaataaaac cgtgcctatt ttcattgtta
    14521 cgggttattt gcatacggct accgaaacag aaatgatctc cgtttccgat aaaaaaagtt
    14581 gcggtgtcat cgaaatactt atgaaaccca ttcgcatgaa aaatctctta gataaaatca
    14641 aagaacatac gttaattgaa aatgctccta aagccgcttt aatgaaaatc taatttgccc
    14701 gctttatctc ctttcttgat aatgagaacc gatagggttg aatgtaccat tcaaccctat
    14761 ttttttacca taaaacactt gcattggcga aataatttta taatatatga gttataagga
    14821 atatgtatta aagcctcacg cacttccctc cttatagtga gaaccgatag gactaggtcg
    14881 ccagacttag tcctagttta ttttttagcc tttttagcgg ctttctttct caattgttta
    14941 ccctgttctg agttgatttt tgtatgtacg tctatataac ccggatattc aatatttaat
    15001 cccgctacgt taatagtcgg cttaactttg acggttatac gcgtaggttt atcaccttca
    15061 ccggccaaat taaatgccaa ttttactaaa ctttcggccg attcattact aaatgatttc
    15121 tttaagtcca tttcgatatt aagcggtacc gtcgcatctg aatccggtaa aatttctact
    15181 atctgatcca aggcacccct aactatttca ttgttatcta taagaacaat ccattcgagt
    15241 cgattcattc cggccggttt ttcattagga ttttcgacag acaagttaat tgtaaatgtc
    15301 aaaggtaacg tttttttggt tagcgcgtcg gttaattgtg cggctttttt taaactcaaa
    15361 tccttggctg actttacatt ttgtatttta atatcagcca gttgcatgtg ttcgatattt
    15421 tgaacggaaa aatcacattt ggcaaacgtt ttcgccatat caatttgttt ggctacacta
    15481 caagaagaca gccaaacagc gaatatacca agtccaatta aacctaattt tttcatacta
    15541 ttaactccgt atttatttga tcctcttttt gacgatttca gaatattcac cgtccggact
    15601 acgtctcagg tattccatga attgcgtttt ggcctgtgca atttgattgg tctttagtag
    15661 tgccatacca aaatagtagt tatttatagc ggcttcctcg tcagtaaagg ctgattgatt
    15721 aaagcatacc ttatccaaaa tacgaattgc gacaggaata tcttgtttta ggaacgcaat
    15781 gatcccttgc atacccgtat aaagaaaatt gaccgaatct acctgtattg cctctaaaat
    15841 ataggattct gctttggtat agtcgttcat tttgatcgct agtatgtaag ccatgcgata
    15901 tttggcatac gcaaagtttg gtcgaatatc aatcgcttta cgataataat tcatagaaat
    15961 ttcatatagc cccaattcct cacaatcctg acctatctcg gcatatatcc gagctttgtc
    16021 gggcatcatt gccgctactt tttcaaattc tgtcgtggcc tgcgctagtt taccccaaac
    16081 agcatataat acacccaaat gcctgtatgc aaaatcgctg tacatatcat attcaatagt
    16141 agctaaaaat tcagattcgg cttttttcat aaatgattcg gcggaatcag gataaatttt
    16201 acgaacttga acagccattt tgagatgaat ccaccctata gtatcgaaat aatatgggtt
    16261 cctctgtgat aattctatcg atttctgcga atatttttca gccacatcaa gttttattcc
    16321 acttaccgca taccgccaag ctatattgtt ataagccccg ttttcctcag gatatttcct
    16381 gatattttca aacataagag aatcgccgcg tttgaaatca tccacagccg tataaatatc
    16441 ggcataaatt tggtcaagat tcggaagcga aggattggct tgctttacta tatcaagcgc
    16501 ttcaatggct ttttttatac gatcggcctt ttcttgtgcc gtcaaaaagt ttacattgat
    16561 ttctgtctga taaagacctg caacactcaa atagttacca atattttccg gttcatatcg
    16621 taaagcgaaa ttcatttgtt catgagcttt ttcaaaatca cccgtttcag ccaaaatact
    16681 ggcataaaat gttcgaggtt gactgatgag tggattgata cgaatggcct gcttgagctc
    16741 attcatggct tcgttatact tcttttgtga aaacaaagca ctggctgaat acagataatt
    16801 tgtgatattg gattcatcta tctgtttggc gcgtcgcgtt atttgtaaga ccttatcata
    16861 atcatattgg gacaataata atcccgcgcg tcttaaaatc aagtcaatac tattaggccg
    16921 catctgaaga agcgtatcca ttatgaccga cgctttatca aattcatcct tctgaagatg
    16981 caaaagcatc attccttcca tcgcgccttc atagtttggc tttagacgca aggattcacg
    17041 ataaagttgt atcgccttat caaaatttcg aactccggaa gctttatcac cctcccattt
    17101 tttttcaacg gcctgatacg aattcagcgg ctcttcggtc acaactaccg gcgtttccag
    17161 gggtaagacg atcgcacatt cattgccgga ctgttgccac tctcttaaaa aatcatcgta
    17221 cggaatggcg tgattatttg ctacagtagg atcatgcata atgatctgat gttttgtttc
    17281 atcatatccg ataacaacga cgacatgacc aaaaccaaaa ggactcaaaa atactaacac
    17341 gggtattccc tgatcaatgc gccgcttgag gtcatctaaa gtaccgacat aaccaaatga
    17401 tttatatttt cctaagcttc tgggataatg aatgatatca ttggactgtg ctcccgcata
    17461 gtcacttttt attttttcat aaatttcgtc ttggtcaatg gattgtttat aatacgacaa
    17521 aaccatagac agcgacgctg cggcacattg agggccgtgc tgtcggacgt agggtatatt
    17581 ttcaagtaca actttttttg gtttttcgag gttttcaatc gtcgtatgaa ttaaatcaac
    17641 agcatatgaa gcgtcttgct gagtcaattt agccgttgcg tcacgaaatt ctttggtcac
    17701 aaagacattt ttaagtttgc gaatcaggcg tttttgtatg tcaaattcga actccgattc
    17761 acctttattc tgaatcgtaa catatcctgt ttcaccatac cgagccgcat cataactccc
    17821 aagaacaccg taatactttt tcccgtaagc aatctgattg ttaatatcat ccagttcagt
    17881 aacagcatca agattgccga gcttaaagat attctgaccg gaacgccata tttcaccttc
    17941 cgaaatcaca cggcgtgtaa aattaccgtg taaatcctcc ataacggcta tgactaatct
    18001 agataaaccc gcaggatctt cttccatcaa tccatatata tttgtcactc cgtagatatc
    18061 ggaaattctg agataaaacc tttgcccctg aattgctttt tcttttgccg caatcgcata
    18121 accattgatt tcaagaatat ctaatgcgaa ctcaacgaca ccgtaagagg taatgtctag
    18181 tacttgctct tggaacgtga taattatttc acgagagaga tcaccattgg cgtctgcacc
    18241 taataatgca tcatttttgg attttatttt taatcggtaa gtattggttt gtccgatatg
    18301 taaggagtcg tcaaagtaaa aacgcgaggg accgatggac tgagaaaatt gacctatacc
    18361 ggcttgctgc agatcgtttt cgtccggcac aaaatattca ggtactcgac gcgataaagt
    18421 ttcatagatt gaaaccgcat tttcataatc ggatttatcg gcatatactt ttgaaagata
    18481 atacaatgca tccccgtccg ccgtgtctat ccgtaaacac tgcagaaatt gttgaatgct
    18541 cttatcgtgc caatccaacc gtgagtaaac caaagccaga ttgtaatgga aattcttttt
    18601 taatttatcg gtatacgcta gctgcaatcc gtgaagtaat gtaggctccg ccgctttata
    18661 ttgcccgttc cgatattgga gcaatcctaa ttgtagatat gcttccgggt acaatgaatc
    18721 ggcagcgatc gctaaacgga gataatatga tgtactgtca aagccacttg tgtgtgctaa
    18781 ggctttatcg aaatatacat acgcttgtgg actgatcttg ccttcattga ttcgtcgaat
    18841 ggtctcaaga gaacttttta ctgagggttg gtcaggtttt atagaaagcg atttttcgta
    18901 atatgcctgc gcctctttca tctggttttt tttgaacaaa aggaaaccca tgtttgcaaa
    18961 atcatctgaa tcggccatgt tatttttggt cagccactca taattaataa acgcttcgtc
    19021 atattcttcc ataacataca aaagccgagc cgctaaatgg cgggataaaa agaaattcgg
    19081 ttcaatttcc aatgattttg ttagggattg ttttgctaaa ccgtaaccct cctgacggtc
    19141 cgtatgcccg ttttgtaccg attcgaaagc aatcatggct ttcagaaaca aattgagtgc
    19201 ttcttctttg aatgtgcctg aaataataag tcttacagcc tgagccaagt ggggaggata
    19261 tttgtcattg atcccctcgc gaatgacctg ttctagatgt ggcaaagcat cattccacaa
    19321 accaacatga agttgacaca gggcatattc atatcgcata tcggtcgtaa catgcagctt
    19381 ttcgcgtaac atccgatcat aaatcgggat cgccatcgca aaatcgcctt gatcatagta
    19441 atattgcgcg agggaactcc aagcataagc attggctgaa tccattttgg taagacgcaa
    19501 aagatcgctt tctatacccg gtttattcag cgcgcgtgcc gcatcagcca aaaaagataa
    19561 gccttgtgca tccgaaggat agttctcatt gtagtacttt gccaattcat aactttcttc
    19621 aaagcggtta agattgtaca aagtacgcgc atacggaagc gcagctcgac ccggttcatg
    19681 acgcaacgct agtgcttttt cgaaatacaa tgccgctttt cgataacttc cctgcttttc
    19741 atacaaagaa gcaatagtca cgtaggcttc ataaaaagtc gcatcttcac gaatcgcgtt
    19801 tttataattt tccagagccg cgtgtatcgc atcagagttt ttcttttcta attttaatcc
    19861 ttccagataa taatgccgtg ctttgtcatt ttgtgtggaa agctgaacct gagagcagcc
    19921 ccaaataacg ataagtgata aaaatggaat tctgattaat ttcatgcgta tggccgatag
    19981 ttaggtaaga aattattccg cacttcgata ttagtcaccg aaatgtaaaa attcaaatag
    20041 aatcgtgtct attttatgat tggattttga aaagtataga ctgttcaacg atagtgtcac
    20101 cggcttcagt tccgattttt tggaaataaa tttgttttaa catattagta aggcattcga
    20161 taaaagcttg atttttagtg cccgaaaaga cggttgtaat gtcaactatt gatccatcgt
    20221 ttttgattac aaaacggaaa cgaactttga aggaagtcgc agttttattg tcaaaatttt
    20281 gactataaca caggctgtag taatttattt tttgctgaat tttttcgctg atttcgcttt
    20341 ccgaacgatt tttactatac acagccggag ctaaacaaaa cacctgcaat ccccctgaac
    20401 gggcataagc attaaattca caacaaaaac ataatattaa tatcaaaaat actcggtaca
    20461 taaactaaat atctctttca ttaaaaacta tcgccgtaaa ggactttata accgttgttt
    20521 tttctttcca tgcatgtttg tttagaccgg ctttgtggca tgtttgttcg ataaacgtta
    20581 tacgatccca ttgatattcg gttgcaacct gaggtaaaag caatccgtga tagaaaccac
    20641 tttcaaccat aagcccgtgc gtcccgacca taatatcgtt gatatcttca acacgagaaa
    20701 atgttgataa aacggatatt tctaactcaa tttcatttaa ctcatccagc tgaagcgatc
    20761 caaaacgatc atcatttaag gccgccgacc gagcagccat aattactgtt ttgtacagcg
    20821 gttccctcgc ttcaatatac ccaatgcacc cgcgtaaatg cccgcgcttc tctatcgtta
    20881 caaatgcgcc ggctttctct tttaatatcg ggaacgtcgc gacatctttt tcataatttg
    20941 gttcttttgt acgctttacg gcagcattta tcgtcatgcg ggctatatgg ataagagttt
    21001 ttttagtttc gttatccaga ttcataacga cctcccgatg tacgaatata gaaccctgcg
    21061 aacagcatcc ataggcattt cttcattagt ccatagctta a
//
LOCUS       DAOQGF010000055        21081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1166_length_21081_cov_6.565918, whole genome shotgun sequence.
ACCESSION   DAOQGF010000055 DAOQGF010000000
VERSION     DAOQGF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_21081_cov_6.565918"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK937_08960"
     CDS             <1..372
                     /locus_tag="PK937_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32740.1"
                     /translation="PQPFQMVIVNPRVDIRESLDVEFYAPMVLNARRYAYQRHGIVFS
                     NYDKETIQHTFENPFIQTELIELFEKCRFKFAVFNERHAIAVKSATVENLARDTEMFP
                     VLMNISTLIAADQHKQIPTLS"
     gene            391..1008
                     /locus_tag="PK937_08965"
     CDS             391..1008
                     /locus_tag="PK937_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNH32741.1"
                     /translation="MSQESGNNSKNLLYAGAALGALLLIGMIILFVKVTSLEKGGSKV
                     GFIKSDALLAQYKPALAIQQKLQEETAPFQADLQKRYAELQTIGEELEKKSKVLSSAA
                     MAPQMESFQRKQSEFYQLQQSLQQQVNQKQSQLMEPIFQDISTFIQKYGKDNGYTVIL
                     GTPVEGLIVYGETGHDLTETLLNELNSKVPPSMPVPFSGKDTTKK"
     gene            1145..2620
                     /locus_tag="PK937_08970"
     CDS             1145..2620
                     /locus_tag="PK937_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH32742.1"
                     /translation="MAKPRTLGELKSSGYQSLSVKDEMRRNLMRKLKQGETIFPNIIG
                     YDKTVIPELIHAILSKHDIILLGLRGQAKTRVLRSLTNLLDEEIPVIEEAPLNDNPFD
                     PVSRAAKDAVEKYGDATPITWWPRHERYGEKLATPDVTVADLIGDIDPIKAATQKLHY
                     ADERVMHFGVIPRSNRGIFAINELPDLQPRIQVSLFNILEEKDFQIRGFNIRIPLDIM
                     IVFSANPEDYTNRGSIITPLKDRIDSQILTHYPTQIEDGIRITRQEAWEDRNPATIAI
                     PDYFREIVENIAFQARGSEFIDQKSGVSARLTISCMENLLSTIERRMILLHEETSFPR
                     ICDLFYLIPSITGKIELVYEGEQQGMASVAKVLIGRAVKATFKQYFPDPLNKKNLPTY
                     QSILSWFSAGNKLELSNEESFASYNAKLLSIPGLRDVAAKYMKTENKDAMTLATAMEF
                     VLEGLHQNSSLSKDWIESHVSYKDIMGTMLGKINWEKEEEE"
     gene            complement(2628..>2825)
                     /locus_tag="PK937_08975"
     CDS             complement(2628..>2825)
                     /locus_tag="PK937_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HNH32743.1"
                     /translation="GDPDKKITAQKMIPWVTSKTRPGGILIFHINGRGYATGKALPEI
                     VTHLRNAGYYFAKLNDYTFAE"
     assembly_gap    2826..2925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2993..3604)
                     /locus_tag="PK937_08980"
     CDS             complement(2993..3604)
                     /locus_tag="PK937_08980"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HNH32744.1"
                     /translation="MEIVLATSNPKKVKEMNAIMSGMQVRFRRLNEFSDVPEIIEDGL
                     TFEANALKKAHVIFEHTGIMALADDSGIEVDALGGKPGVFSARYAGPAQDDRANLEKL
                     LRDTEHVPDEKRTARFHCVIALVWGQAGNVMEKTFHGVMEGALLRAPRGTHGFGYDPI
                     FMVPQYDGKAAAELDPDLKNKISHRGRALEQFKTFFEKEFFIR"
     gene            3737..4129
                     /locus_tag="PK937_08985"
     CDS             3737..4129
                     /locus_tag="PK937_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH32745.1"
                     /translation="MEFSKEEMMEIMAVFKAESAENLQILNDKMKEIAANPSNPAAIE
                     QLHRTSHSMKGAARMLGLTPIEEIGRALEDGFKVAKDGKPLLTPDMIENVNVAVLGMA
                     QLIDKLANEGTTDGFDISGIMQRLAAFK"
     gene            4131..4895
                     /locus_tag="PK937_08990"
     CDS             4131..4895
                     /locus_tag="PK937_08990"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH32746.1"
                     /translation="MTPELMPGAEPFFYTSETPAHNAVLLVHGFTSSCHSMRELGKRI
                     YENGMDARGILLPGHGTTPDDLATRYWSEWYEAVNIAFLEMKKNYNKVFIAGQSMGGC
                     LALLLAARNRAVDGVVTIASGLKIYNTGIGLVPLVKHFIKHKPKLYGPDVKDERVKQT
                     EVHYTRMPYASIHELLKLLKSLRAEIGNVTCPLLTIHGREDHTFDFRNQDMIYRRVAS
                     TQKKKVVLENTYHLATLDFDKTIVQNETINFLKSLC"
     gene            4889..5200
                     /locus_tag="PK937_08995"
     CDS             4889..5200
                     /locus_tag="PK937_08995"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNH32747.1"
                     /translation="MLNHETATDVTIDRGKKLVTIQWADGKLSELPYAYLRAACPCAN
                     CDTTKHGGEANPLYPEQFADITMSDVQEVGRYALRFTWSDGHDTGIYSYSYLREKSNL
                     F"
     gene            5222..5947
                     /locus_tag="PK937_09000"
     CDS             5222..5947
                     /locus_tag="PK937_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNH32748.1"
                     /translation="MRLMIVTIAAVLTVVSCKTTQPAAKSDADFAKALETSEKNKSEK
                     LDFLADEKLPNDMITPPDTASPMTPAPDSITYKTYEPTIQARFRVQVFAGSPSNAQKN
                     YTSLSADTTWKKAVYVLDDKTDGKWRVWVGDFMTREEADAAKQKLIQNGYPDAWIHEM
                     KDMTTPKISGKELFWLQIGSFSTEAAAQKAKTEAESKQTVRSKISHTDGMYKVWIGGF
                     EDRAKADELKKTFSKAFIVKGAE"
     gene            5951..6610
                     /gene="ftsE"
                     /locus_tag="PK937_09005"
     CDS             5951..6610
                     /gene="ftsE"
                     /locus_tag="PK937_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNH32749.1"
                     /translation="MIELSNVTVRYRNRTSLENASLRIAPGEFVYLLGPSGAGKSTLL
                     KLLYMDIIPEEGQVRIGEFESSNVKKKEIPYLRRQLGIVFQDFRLLRDRSVYDNIAFT
                     LLVTETPKSEIPKKVMRALAQVGLSTKKNSMPYELSGGEQQRVVIARALVNEPMVILA
                     DEPTGNLDPDTSMEIMQLLTKINAEGTAIVMATHDYNIVKALPKRIIRVEGGKITDAP
                     N"
     gene            complement(6612..7133)
                     /locus_tag="PK937_09010"
     CDS             complement(6612..7133)
                     /locus_tag="PK937_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32750.1"
                     /translation="MKSIQLLSQLELTCIKPDDHQKRFQIMDGADVVGELSYAKSAGT
                     LARAESGDAIWTFKRCGLWKPYISIRKEGTHTNLARIILGWHNVLMSEPPLRRVHFRN
                     SSFWHNEWSWVTDEDETIMKFKPAHDHHMRGKIMIHEKFRQDGWLMMMALLGWYMLVS
                     LEDEAGDTMVVMS"
     gene            complement(7139..8542)
                     /locus_tag="PK937_09015"
     CDS             complement(7139..8542)
                     /locus_tag="PK937_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32751.1"
                     /translation="MKKISSQISYATIALFLVGIVALLFVVDNLLRKNLQNELIENLK
                     VHCLLIDELLPVQPDSLEREIPMYSRMAGVRITVMDIQGRVLADFDGMRFTRTIENHI
                     MRPEIQMALSDVAGFGYAIRHSGTVDQDLVYTVYKSPKQRFVRVAQNQSFIDGITSQV
                     RFTFYLASVLVVGLMLFLTVRIAKRITRPLTEIIRASSEIKSGNYNKEIIVREDNELG
                     ELGRTLNAMSAKLKDDIKQLNKVQEIRKDFVANASHELRTPISSVRGYIETLLDGAYH
                     DEEVSKKFLERAHSNILRLENIVNDMLDLSRLESRDQSLSLRYFNVEQSLRNIVADFE
                     EVARRKNLDLACTVNLPLDFKFIADPYQFDKAVLNLVENAVKYTEKGRVDVRAKFENK
                     KLVVEVEDTGNGIKQEDTERIFERFYRVDRGRSREQGGSGLGLAIVKHVMEIHKGTVR
                     LESEVGKGSRFILTFPV"
     gene            complement(8545..9252)
                     /locus_tag="PK937_09020"
     CDS             complement(8545..9252)
                     /locus_tag="PK937_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32752.1"
                     /translation="MSKKPKTILCVDDEHDILDILTYNLKKEGYQVYTADNGDKAITM
                     AEKIRPDMVLLDIMMPGKDGLEVCKALRANPHLKDAHVIFLTAKADEVDEIIGLEFGA
                     DDYVTKPFSPRKIVARVKALFRRVEVQKELTDDKKSIIKIGGIEINRINYTVQVDGQE
                     VKFLHKEFELLYFLMNRPNMVFSRNNLLYHVWGEDAFFVDRTVDVHITKIRKKLGPYG
                     DWIETIRGVGYKFIQNA"
     gene            complement(9258..9929)
                     /gene="phoU"
                     /locus_tag="PK937_09025"
     CDS             complement(9258..9929)
                     /gene="phoU"
                     /locus_tag="PK937_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNH32753.1"
                     /translation="MKAQFTEQLGQLEKLVIEMGSVVESQVNNVVTALLTHNSELAQT
                     VIKQDETVDALEMQIDEAVLNLLALHQPVAMDLRDIIGISKINGDLERIGDHALNIAQ
                     SVLVINERNYRGIHAQISKMADITREMLKDAIDSFIHKDADQAIAVCRRDDTIDDINK
                     FMFADVLAGMIKDPQSIPISMEIIRISKNFERMADLCTNIAEDVVFIRNAKSIKHQKE
                     LLQPK"
     gene            complement(9981..10781)
                     /gene="pstB"
                     /locus_tag="PK937_09030"
     CDS             complement(9981..10781)
                     /gene="pstB"
                     /locus_tag="PK937_09030"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNH32754.1"
                     /translation="MDNSALGESTPEQVSHRVKIVSRDINMYYGAHHALKNISVDIHA
                     NEVTALIGPSGCGKSTYLRLFNRMNDLIENVRIEGQILMEGQNIYDKDTDVVELRKKV
                     GMIFQKSNPFPKSIYENVAYGPRVNGENNKTKLEGIVERCLRKSALWDEVKDRLHASA
                     YGLSGGQQQRLCIARALAVDPEVLLMDEPCSALDPIATQKIEDLIEELKRDYTIVIVT
                     HNMQQAARVSDKTAFMWMGELIEFNATKKIFTSPEKKMTEDYITGRFG"
     gene            complement(10823..12448)
                     /gene="pstA"
                     /locus_tag="PK937_09035"
     CDS             complement(10823..12448)
                     /gene="pstA"
                     /locus_tag="PK937_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNH32755.1"
                     /translation="MKNLFKSGDIFLWFTGAVLGMIMFLVAGLLLIVSLQGVGVFWPG
                     SVAEVELKDGRRFAGEIMQEENIPLANVPDAYKEAVQTRIQVKTGNRDIYGLDFKWLN
                     TYDIASLQYPDDFAIIERREFGNFYGRVASISENGTIIASGAQATETLDQFLRKALDV
                     HDEQLHLEKGEGGRINRAIEDTRLEIKRLKFYKEDTPELLSSLEAEIHRLQEAYQGIQ
                     KRLETLHLKTSGMYVSVITTDGQTRDLPVSKIVRFYQPNTMNTFEKSGFYISKIFEYL
                     TDEPRESNTEGGVFPAIFGTVMMVFLMNLVVVPFGVVAALYLREYAKQGIAVRLIRIA
                     VNNLAGVPSIVFGIFGLGFFVYFCGGTIDQLFYPDRLPTPTWGTGGILWASLTLSLLT
                     VPVVIVATEEALTTVPKGVRDASLALGATKWQTTWRVVIPASTPGILTGVILAMARGA
                     GEVAPLMITGVVKLAPNLPLDGSFPFFHLDRKFMHLGFHIYDVGFQSPNVEAAKPMVF
                     MTALLLITIVLLLNLTAIIVRARLRKKYQGSAF"
     gene            complement(12461..14635)
                     /locus_tag="PK937_09040"
     CDS             complement(12461..14635)
                     /locus_tag="PK937_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH32756.1"
                     /translation="MNFLNRRHIYDRLASGIIAAGGFALIVIILGMMGFIIKQALPLW
                     SGAELSTASVIELAQDTNDKSVIKLIGQEEQREILFALTDSGAVRFMRTSDQKELQTF
                     HIPLHKDEHIIHAMEAFASHRIACVTDQGRIIEAEIKFSSQFTGDHRSIIPELHILSV
                     RTFSKTKTPVRGISYAVTASDAYVAVAKENTAYYYKSSDTVPSLVVTDAVIRSMAINP
                     LREEIVLGLEDGHMERWDIRETNAPTRIESVAATAQNGIAVTALAYLIGDISLIVGDE
                     EGRVSGWMEVKHTDGSKNFARIRDFDRHESSIQAITASWRNKCFVTADGRGVFKLFHS
                     TSDRLLVEHKLSYAPSQVSYAPKADGLVFLSGEGRVENFALHNPHPESNWQTLFGEVW
                     YESYDAPGYTWQSTGGTDDFEPKFSLIPLIFGTLKATLYAMLFAAPLAILGALYTSQF
                     AHPRIRTIVKPTVEIMAALPSVVIGFIAGLWLAPLLKNVLIEFIAVITLLPLLIILFV
                     LLFDKLPKKWTNPFRHGYEVFLMLPVLAISIWIGYMVGYWIETNMLGGDVQNWLYQTL
                     GIRYDQRNSIIIGIAMAFAVMPIIFTITEDALSSVPKHLTSASLALGATRWQTAIKVV
                     LPAASAAIFSAVMIGFGRAVGETMIVLMATGNTPIMDWSIFNGMRTLSANIAVEIPEA
                     PEGGTLYRVLFLSGLLLFVITFVVNTLAEIVRQRLRKKYASM"
     gene            complement(14632..15561)
                     /locus_tag="PK937_09045"
     CDS             complement(14632..15561)
                     /locus_tag="PK937_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNH32757.1"
                     /translation="MSNEVLMWIGFNVFVLLMLALDLGIFHKKSHSVGMKEALIWSGV
                     WITLAMVFNIGVYHYMGSEAAMQFLTGYVIEKSLSVDNIFVFVLIFSYFAVPAEYQHK
                     VLFWGIIGALIMRAILIAVGAALISEFHWIIYIFGGFLIITGIKMLVQKEMEIHPESN
                     PVVRFFKKFMSVTPSFEGDRFFVKKDAKTWATPLFVVLLIVEFTDLIFAVDSIPAIFA
                     ITTDPFIVYTSNVFAILGLRSLYFALASVIEKFHLLKLGLALVLIFVGVKMTIVDFYK
                     IPVVIALAVVASILSVSVILSLLIPAKSKTEKS"
     gene            complement(15647..16699)
                     /locus_tag="PK937_09050"
     CDS             complement(15647..16699)
                     /locus_tag="PK937_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNH32758.1"
                     /translation="MKHMQQLLASAVFAGMMMLGACGDKKKEIKNETADPSKTTTAVA
                     ASTAVKVDEGIVAYAKTSGVSGNLNSIGSDTMNNLMTFWAEAFKKFYPSVNVQIEGKG
                     SSTAPPALIEGTAQLGPMSRPMKKEEEDKFEKKYGYKPTGIATSYDGLAVYVHKDNPI
                     KGLTLQQVDAIFSKTRKGGAKEDVVTWGQLGLEGEWKDKPISLYGRNSASGTYGYFKD
                     HALFKGDFKDQVKEQPGSASVVQGVGSDKYAIGYSGIGYTTSEVRAIPVAKDDKSEFV
                     GESYDNVKSGKYPLSRYLYVYVAKHPTKDMDPLTKEFLKFILSKEGQEVVVKDGYMPL
                     TADVVKQQLELVEKKK"
     gene            17040..18119
                     /locus_tag="PK937_09055"
     CDS             17040..18119
                     /locus_tag="PK937_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32759.1"
                     /translation="MKKMFLLLALCVATASIAQDQPAGKVHGYVFGDYYYKMGGDKAK
                     AQSNTQYSDPALRKKSAFQLRRMYLYYEHDLSSNFHATFLLEGNDKAMEWAEDTSKAQ
                     VPGRHSVFIKLANLEWKDYIKDHSIAIGLIPTATWSLSEKYWGYRAIEKTITDYRGLG
                     GASDIGIVAKGKFLDDGMIGYTLSVVNGNGQKPENNKSRKYIGSLSVKPIKSVITEIY
                     ADYEGSITEPKKYGKVDSRNTLKALVAYQSDIATTGLEVVQQTQKRTDTTDVKPFGFS
                     VFASAPVMKEKLNVFARYDSFDPNTEITKAGFNEYFFTAGVDYMPIKNVHFMPNIWIN
                     GFSAKKGSSRDADVVARLTFFYVYK"
     gene            18253..18582
                     /locus_tag="PK937_09060"
     CDS             18253..18582
                     /locus_tag="PK937_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNH32760.1"
                     /translation="MKTNKKKQIPKNYQNFVKDYPRVAKAYEELGDAVHHAGPLDTKT
                     RALIKLAISAASRQEGAVHSHVRKALTAGASPEEMRHVIMLTLPTIGFPGMMAAMSWV
                     NDIISSK"
     gene            18687..18941
                     /locus_tag="PK937_09065"
     CDS             18687..18941
                     /locus_tag="PK937_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH32761.1"
                     /translation="MMHIQIAGPGCINCTKLEDMCREAIHAYHVDAQIERITERQDYS
                     RLGVFATPGLLINGHIISTGRMPAYLTVEKWIKSAEDWSS"
     gene            19169..19738
                     /gene="msrA"
                     /locus_tag="PK937_09070"
     CDS             19169..19738
                     /gene="msrA"
                     /locus_tag="PK937_09070"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNH32762.1"
                     /translation="MRKEPMITNQAAVDTATFGAGCFWCVEAVFQRTKGVLSVKSGYS
                     GGHVDNPTYQQVCNGNTGHAEVIQLTFDPKTVSFVELLEVFFGTHDPTTLNRQGADEG
                     TQYRSAIFYHSDDQRRIAEDVKQKLDASGAFKNPIVTEISAFNKFYPAEDYHQNYFND
                     NGNAPYCAYVIKPKLEKFRKVFKDKLKAD"
     gene            complement(19900..20958)
                     /locus_tag="PK937_09075"
     CDS             complement(19900..20958)
                     /locus_tag="PK937_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH32763.1"
                     /translation="MLISDRGMMAWDTRRNRHWIKQAVKRDVLIGDIALSISARYKMS
                     SQTATISGPGGFNKKGGLTYTVSSASAGTSLQYTWYLKYDFNSVWQNIGSGTSKFINI
                     SALSNAYNFNLRVYMRNLTDTIGVWSNEYRVTCLSCGGGYNDPNQRRDMADAGAEEDS
                     VTHGEWWPVNADTLTMHHKRRILMPIRDAIRMMNHRFRWKKNDMFTIGTNDDLIEDEE
                     SAYTEKNEPNESSIITEHIAQNDKISKKQENGDPAMPFDQTPKRFALYEAYPNPFNPS
                     TTLAFDVIEKSEVSLIIYNTLGQTVRRLITETRSSGHHRVLWDGKNDSGVLVPSGVYI
                     YQLRAGNNIQSRKMIFMK"
     gene            complement(20879..>21081)
                     /locus_tag="PK937_09080"
     CDS             complement(20879..>21081)
                     /locus_tag="PK937_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32764.1"
                     /translation="RTGNTSLIEGLGFLGPNTRTVELPYRIAQVDADGEPTGGTYAYF
                     RSWDDGVGYAPQSPLDQASGKT"
BASE COUNT         5840 a         5103 c         4522 g         5516 t
ORIGIN      
        1 ccccagccgt ttcagatggt tattgtcaat ccgcgtgtgg atattcgcga atctttggac
       61 gtagaatttt atgcaccgat ggtgctcaat gcgcgccggt atgcatacca acgccacggt
      121 atcgtttttt ccaattatga taaagaaacg attcaacata cgtttgaaaa tccttttatt
      181 cagacggaat tgatcgaatt attcgaaaaa tgccgattca aatttgcagt attcaatgaa
      241 cgtcacgcca tcgctgtcaa gtccgcaacg gtcgaaaatt tggcgcgtga tacggagatg
      301 tttcccgtgc tgatgaatat cagcaccttg atcgccgccg atcaacacaa acaaattcct
      361 acattatcat aaaacaacaa ggagaatcct atgtctcaag aatccggaaa taattctaaa
      421 aacctgcttt acgccggcgc ggcgctcggc gctttgcttc tgatcggtat gatcattttg
      481 ttcgtcaaag taacttcgct cgaaaaaggc ggttccaaag tcggcttcat caaatcggat
      541 gcgttgctcg cacagtacaa acccgcgctg gcaattcagc aaaaactgca agaagaaaca
      601 gcgcctttcc aagctgactt gcaaaagcgt tatgcggagc tgcaaaccat cggcgaagaa
      661 cttgaaaaaa aatctaaagt cttaagttcg gccgctatgg caccgcagat ggaatccttt
      721 caacgtaaac aatccgaatt ttaccaattg cagcaatcac tgcagcaaca ggtcaatcaa
      781 aaacaatccc aactgatgga acctattttc caggacatca gcacgtttat tcaaaaatac
      841 ggtaaagata acggatatac cgttatcctt ggcacgcccg tcgaaggatt gatcgtatac
      901 ggcgaaaccg gccacgacct gacagaaacg ctgctcaacg aactcaactc caaagtacct
      961 ccgtccatgc cggtaccttt cagcggtaaa gacacgacga aaaaataaat atatctacat
     1021 tgttgacata agccgcttcg ttaaaccgaa gcggcttttt ttatcagcac aattctcaaa
     1081 cctgagcgtt gaagatcatc tataatcgtg ttagattagg gccactttac aatagaggat
     1141 atatatggca aaaccgcgga cgctcggcga actcaaatct tcaggctatc aaagtttatc
     1201 ggtcaaagat gaaatgcggc gcaatctgat gcgcaaactg aaacagggag agacaatttt
     1261 ccccaatatc atcggctatg ataaaaccgt tatacctgaa ctcattcatg ctattttatc
     1321 caaacacgat attatcctat tgggattacg cggacaagcc aagacacgtg ttctgcgcag
     1381 cctcaccaat cttttagacg aagaaatccc cgtcatcgaa gaggcaccgc tcaacgacaa
     1441 cccctttgat ccggtctcgc gtgcggccaa agatgccgtg gaaaaatacg gtgatgccac
     1501 accgatcaca tggtggccgc gccatgaacg ctacggcgaa aaattggcca cacccgatgt
     1561 aaccgtcgcc gatctgatcg gcgatatcga cccgatcaaa gcggctaccc aaaaacttca
     1621 ctacgccgat gaacgcgtca tgcactttgg cgtcatccct cgcagcaacc gcggcatttt
     1681 tgccatcaat gaattacccg atctccaacc gcgcattcag gttagtttgt ttaatatttt
     1741 ggaagaaaaa gattttcaga ttcgcggatt caatattcgt atcccgctgg acatcatgat
     1801 tgtcttttcg gcaaatcccg aagattatac caatcgcggc agcattatca cgccgctcaa
     1861 agatcgtatt gactcgcaaa tcctgaccca ttaccccaca caaatagagg acggcattcg
     1921 tatcacacgt caggaagcgt gggaagatcg aaatccggct acgatcgcca tacccgatta
     1981 tttccgtgag attgtcgaaa atatcgcctt tcaagcgcgc ggcagtgaat tcattgatca
     2041 aaaatccgga gtaagcgccc ggcttacgat ttcgtgcatg gaaaaccttc tgagcaccat
     2101 cgagcgacgc atgatactat tgcacgaaga aacatctttt ccgcgtatct gcgatttgtt
     2161 ttatctcatc ccttcgatca ccggaaaaat cgaactcgtc tatgaaggcg aacaacaagg
     2221 tatggcctct gtcgccaaag tattgatcgg gcgtgccgtc aaagcgacat ttaaacaata
     2281 ctttcccgac ccgctcaaca aaaaaaatct cccgacgtac caatccatcc ttagttggtt
     2341 ttccgccggt aacaaactgg agctttccaa cgaagaatct tttgcatcgt acaacgccaa
     2401 attattatcc attcccggct tgcgcgatgt cgccgccaag tacatgaaaa cggaaaacaa
     2461 agacgcgatg actttggcaa cagcgatgga gtttgttttg gaaggccttc atcaaaattc
     2521 gtcgttaagc aaagactgga tcgaatcgca tgtaagctac aaagacatca tgggtacgat
     2581 gctcggtaaa atcaattggg aaaaagagga agaggaataa aaatcactta ttcggcgaag
     2641 gtgtagtcat taagcttcgc aaagtagtaa ccggcgttac gcagatgcgt gacgatttcc
     2701 ggtaaagcct tgcctgtagc atacccacga ccgttgatat gaaaaatcaa aatacccccc
     2761 ggccgtgttt tggatgtaac ccacggaatc atcttttgtg ccgtaatttt tttgtccgga
     2821 tccccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgca gctcaccacg
     2941 ataccgatga gcgtcaccac tagaacggat gtaattttca aaaggttatt tttcatcgta
     3001 taaaaaattc tttttcaaaa aatgttttaa actgttccaa cgctcgaccg cgatgactga
     3061 ttttattttt aagatcagga tctaattccg cggcggcttt tccatcgtat tgaggcacca
     3121 taaaaatcgg atcatagcca aaaccatgtg ttccgcgcgg cgcacgcaga agcgcacctt
     3181 ccatcacacc gtgaaatgtt ttttccatta cattaccggc ttgaccccat accagtgcga
     3241 tgacgcaatg aaagcgtgcg gtacgctttt catccggcac atgctctgta tcccgtaata
     3301 acttttccag gttagcacgg tcgtcttgtg ccggaccggc ataccgtgcc gaaaaaacac
     3361 cgggttttcc gcccagcgca tccacttcga tacccgagtc atccgcgagc gccattatcc
     3421 ccgtatgctc aaatatcacg tgcgcttttt ttaatgcatt ggcctcaaac gtcaatccgt
     3481 cctcaatgat ttccggtaca tcggaaaatt cattcagccg tctgaaccgg acttgcatcc
     3541 cgctcataat agcattcatt tcttttactt ttttgggatt acttgttgct aaaacaattt
     3601 ccatcgtgtg ctccgttgta ttcaccaatg tcataaaaaa agagcaaagg ctaaaggaat
     3661 tattcgtgcg gaaaacaaat tgaagttttt agagaaatcg cctacattca ccgacctgaa
     3721 acattccgag gtgtgtatgg aattttccaa agaagaaatg atggagatta tggccgtttt
     3781 caaagccgag agcgccgaaa atcttcagat acttaacgat aagatgaaag aaattgctgc
     3841 gaacccttcc aatcctgcag cgattgagca gcttcaccgc acttcccaca gcatgaaagg
     3901 cgcagcacgt atgctcggtc tgacccctat cgaagagatc gggcgcgcgc tggaagacgg
     3961 attcaaagtc gccaaagacg ggaaaccgct cctaacgccc gatatgatcg aaaacgtgaa
     4021 tgttgccgtc cttggtatgg cgcaactcat tgataaattg gccaatgaag gcactactga
     4081 cggctttgat atcagcggta ttatgcagcg cctcgcggcg tttaagtgat atgaccccgg
     4141 agctcatgcc cggcgccgaa ccgttctttt acacttcgga aaccccggca cataacgccg
     4201 tactattagt ccacggtttc acttccagtt gccactcgat gcgcgaactc ggaaagagaa
     4261 tttatgaaaa cggcatggat gcacgcggca tactgcttcc gggacacggt actacacccg
     4321 acgacctcgc tacacgttat tggtcggaat ggtatgaagc ggttaatatt gcttttttag
     4381 aaatgaaaaa aaattacaac aaagttttta tcgccggaca atctatgggc ggatgcctcg
     4441 ctttattgct cgcggcgcga aaccgcgctg tagacggtgt agttaccatc gcatcgggat
     4501 taaaaatcta taataccggc attgggttgg taccgttagt caaacatttt ataaaacaca
     4561 aaccaaaatt gtacgggccg gatgtcaaag atgaacgcgt caaacaaacg gaagtgcatt
     4621 acacacgcat gccgtatgcc agtattcatg aattgctcaa actgttgaaa tcgctgcggg
     4681 ccgagatcgg caatgtaacc tgcccgttgc ttacgataca cgggcgcgaa gatcatacgt
     4741 ttgattttcg caatcaagac atgatttatc gtcgcgtcgc ttcgacccaa aagaagaaag
     4801 ttgttctcga aaacacgtat catttagcca cgttggattt tgataaaacg atagtacaaa
     4861 acgaaaccat aaattttttg aaatcattat gctaaatcac gaaaccgcta cggacgtcac
     4921 gatagaccgc ggtaaaaaat tagtcacgat ccaatgggcc gacggaaaac tcagcgagtt
     4981 accgtacgcc tatctgcgcg cagcatgccc ctgcgccaat tgtgacacga ccaaacacgg
     5041 cggagaagcc aacccactgt atccggagca atttgcggat attacgatgt cggacgtgca
     5101 ggaagtcggg cgctatgctc tgcgttttac ctggtcggac ggccatgaca cgggcatata
     5161 ttcatacagc tatttgcgcg aaaaaagtaa tttgttttaa tacacctatc ggagaaaatg
     5221 tatgcgtttg atgattgtga ccattgcggc tgtgcttacg gtagtttctt gtaaaaccac
     5281 gcagcctgcg gctaaatccg atgccgactt tgccaaagcg cttgagacgt cggaaaaaaa
     5341 taaatcagaa aaattagatt ttttggccga tgaaaaactg cccaatgata tgatcactcc
     5401 cccggatacg gcaagcccga tgacgcctgc gcccgattcg atcacttata aaacttacga
     5461 gcctacgatc caggcgcgtt tccgcgtaca ggtgtttgca ggcagtccct ccaatgcgca
     5521 gaaaaattat acatcgctct ccgccgatac cacatggaaa aaagccgtgt atgttttgga
     5581 tgacaaaacc gacggcaagt ggcgcgtctg ggtcggcgat tttatgacac gggaagaagc
     5641 cgatgccgcc aaacagaaac taatacaaaa cggttatccc gatgcgtgga ttcatgaaat
     5701 gaaagatatg actacgccga agatttccgg taaagaactt ttctggcttc aaatcggatc
     5761 tttttctact gaagcggcag cgcaaaaagc aaaaacggaa gccgaatcca aacaaacagt
     5821 acgctctaaa atttcccata ccgacggtat gtacaaggta tggatcggtg ggtttgagga
     5881 ccgcgcgaaa gccgatgaat tgaaaaaaac gttttccaaa gcgtttatcg taaaaggcgc
     5941 ggagtaaccg atgatcgaac tttcaaacgt aaccgtacgt tatcgcaatc gcaccagctt
     6001 ggaaaacgct tcgctacgca tcgctccggg tgagttcgta tatttgttgg gacctagcgg
     6061 tgccggcaaa agcacactgc ttaaattatt atatatggat atcataccgg aagaaggtca
     6121 ggtgcgcatc ggtgaatttg aatcatcgaa tgtcaaaaaa aaggaaatcc cttacctgcg
     6181 ccgacagctg ggtattgtat ttcaggattt tcggttatta cgggatcgtt cggtttacga
     6241 taatatagcg tttacgttgc tcgtcacgga gacgcccaag agcgaaattc cgaaaaaagt
     6301 gatgcgtgca ctggcccaag tagggctttc caccaaaaag aacagcatgc cctacgaatt
     6361 atccggcggc gaacaacagc gcgtcgtcat cgcacgcgca cttgtcaacg aaccgatggt
     6421 gatcctcgca gacgaaccga ccggtaatct tgatccggat acttcgatgg agatcatgca
     6481 gctgctgacc aaaataaacg cggaaggcac agccatcgtt atggccaccc acgattataa
     6541 catcgtgaag gcgcttccca aacgcattat ccgtgtcgaa ggcggcaaaa tcaccgacgc
     6601 acccaactga atcaggacat cacgaccatc gtatcgccgg cttcatcttc aagcgaaacc
     6661 agcatatacc agcccagcaa tgccatcatc atgagccatc cgtcctgacg aaatttttca
     6721 tgaatcataa tttttccgcg catatgatga tcatgcgccg gtttgaattt catgatcgtt
     6781 tcatcttcat cggtcaccca cgaccattcg ttgtgccaaa agctcgaatt gcgaaaatgt
     6841 acgcgccgga gcggcggctc cgacatcagc acattgtgcc atccgagaat gatgcgcgcc
     6901 agattggtgt gcgtgccctc tttacggatt gagatgtacg gcttccaaag accgcatcgt
     6961 ttgaacgtcc aaatcgcatc gccggactcg gcgcgggcaa gtgtgcccgc cgatttggca
     7021 tagctcagct caccgaccac gtcggctcca tccatgatct gaaatcgttt ttgatggtca
     7081 tcgggtttga tgcaggtaag ctcaagctgc gagagtaatt gtattgattt catacgactc
     7141 acacaggaaa agtgagaata aagcgggaac ctttcccgac ttcactttcg agacgtacag
     7201 ttcccttatg aatttccata acatgtttga caatagcaag ccccagaccg ctcccgcctt
     7261 gttcccgtga acgaccacgg tccacacgat aaaaacgttc gaaaatacgc tccgtatctt
     7321 cttgtttgat accgttgccg gtatcctcaa cttcgaccac tagtttctta ttctcaaact
     7381 ttgcgcgcac atccacgcgc cctttttccg tatatttgac ggcgttttct acgagattca
     7441 atacggcctt atcaaactga tacgggtccg cgataaattt gaaatccaag ggcaaattga
     7501 ccgtacatgc caaatccaga tttttacgcc gtgccacctc ttcaaaatca gcaacgatat
     7561 tacgcagact ttgttcaaca ttaaaatagc gcagactcag cgattgatcg cgggactcca
     7621 aacgggaaag atccagcata tcattgacga tattttcaag acgcaaaata ttagaatgcg
     7681 cacgttcaag aaattttttg ctcacttctt cgtcatgata cgcaccgtcc agcaacgttt
     7741 cgatgtaccc gcgtaccgaa ctgatcgggg tacgcaattc atgcgaggca ttggccacaa
     7801 aatcttttct gatttcctgt actttattga gctgtttgat atcgtctttc aatttggccg
     7861 acatcgcatt gagtgttcga cctaattcac cgagttcatt gtcttcacgg acaatgatct
     7921 ccttgttata atttccggat ttgatttccg atgaggcgcg tatgatctcg gtcaatggac
     7981 gcgtgatgcg tttggctatg cgaacggtaa gaaacaacat caatccgacg accaatacgg
     8041 acgccaaata aaacgtaaat cggacttgtg atgtaatacc gtcaatgaac gattgatttt
     8101 gagcgacgcg cacaaaacgc tgtttgggcg acttatacac cgtataaacc aagtcttgat
     8161 ctacggtacc ggaatgccgt atcgcataac caaatcccgc cacatcgctc agcgccatct
     8221 ggatctcggg acgcatgata tgattttcga tggtgcgtgt aaaacgcata ccgtcaaaat
     8281 cagccaatac acgaccctgg atatccatca cggtgatacg cacgccggcc attcgcgaat
     8341 acatagggat ttcccgttca agcgaatccg gctgtaccgg caatagctcg tcaatcagta
     8401 aacaatgaac tttaaggttt tcgatcagtt cgttttggag attttttcga agaagattat
     8461 caacgacgaa aagaagcgct acgattccta ccaggaaaag tgcgatcgtc gcatatgaaa
     8521 tctgcgaact gatttttttc atgatcaggc gttttgtatg aatttataac ccacaccgcg
     8581 aatggtttcg atccaatcgc cgtacgggcc taattttttt ctgattttag taatatggac
     8641 atccacagtg cgatccacaa aaaatgcatc ttcgccccac acatggtaaa gaagattatt
     8701 tctagaaaac accatattgg gccggttcat cagaaaatac aacaattcaa attctttgtg
     8761 gagaaatttt acttcctgcc cgtccacttg cacggtatag ttgatccggt tgatttcgat
     8821 accgccgatt ttaatgatgc tttttttgtc gtccgtaagt tctttttgga cttcgacacg
     8881 gcgaaacagc gctttcacgc gggctacgat tttgcgaggg ctgaatggct tggtcacata
     8941 gtcatcggca ccaaattcaa gcccgataat ttcatccact tcatcggctt tggcggtcag
     9001 aaaaataacg tgcgcatctt taaggtgcgg attcgctctc agcgctttac acacttccaa
     9061 tccgtctttg cccggcatca tgatgtccag taaaaccata tcgggtcgaa ttttttccgc
     9121 catggtgatt gctttatcgc cattgtccgc ggtatatacc tggtatcctt cttttttgag
     9181 attgtacgtt agaatatcca ggatatcatg ttcgtcgtca acacacagga tggtttttgg
     9241 ttttttggac atacatttta ctttggttgt aatagttctt tctgatgttt gatagacttt
     9301 gcattacgaa taaacacaac gtcctcggca atattggtac aaagatcagc catacgctcg
     9361 aaatttttac tgatgcgaat gatctccatc gaaatcggaa tactctgcgg gtctttgatc
     9421 attccggcca atacatccgc aaacatgaac ttattgatat cgtcaatcgt atcatcacgt
     9481 cggcataccg cgatagcctg atcggcgtct ttgtgaataa aactgtcaat cgcatctttg
     9541 agcatttccc gcgtaatatc ggccattttg gatatctgtg catggatgcc gcggtaattg
     9601 cgttcattga tcaccagcac ggactgcgcg atattgagcg catgatcgcc gatgcgctca
     9661 agatcaccgt tgatcttgga aataccgatg atgtcgcgta aatccatcgc tacaggttga
     9721 tgcaaagcga gcagattgag tacggcttcg tcaatttgca tttccaacgc atcgactgtt
     9781 tcgtcctgtt tgatcacggt ctgtgctaat tccgaattgt gcgtcagcaa cgctgtcacg
     9841 acgttattga cctgcgattc aacgacgctg cccatttcga tcacaagttt ttcaagttgg
     9901 ccaagctgtt ccgtaaactg agctttcatg tattacaatc ctttattctc aatacttttc
     9961 atggttaggt ttatgttttt ttatccgaag cgacccgtga tataatcttc ggtcattttt
    10021 ttctcagggg aagtaaatat ttttttggtg gcgttaaatt cgatcagctc gcccatccac
    10081 ataaatgccg ttttatccga cacgcgggcc gcttgttgca tattgtgcgt gacgatgacg
    10141 atggtataat cgcgcttcaa ttcttcgata agatcttcga ttttttgtgt ggcgatcgga
    10201 tccaaagctg aacaaggttc atccattaat aacacttcgg gatccacggc tagcgcacgg
    10261 gctatacata aacgctgttg ctgaccgccg gacagtccgt atgctgatgc atgaaggcga
    10321 tctttcactt cgtcccacaa ggctgacttt cgtaaacacc gttcgacgat gccttctaat
    10381 ttggttttgt tattttcacc attgacgcgc ggaccgtatg ccacattttc atagatggat
    10441 ttgggaaacg gattggactt ctgaaaaatc atgcccactt ttttccgaag ctccaccacg
    10501 tccgtatctt tatcatagat gttttgtcct tccatgagaa tctgaccttc gatacgtacg
    10561 ttttcgatca gatcgttcat ccggttaaat aagcgcaggt atgttgattt accgcaaccc
    10621 gacggaccga tcaaagcggt tacttcgttg gcatgtatat ccaccgaaat gtttttgagc
    10681 gcatggtgcg cgccgtaata catattgata tcgcgcgaaa cgattttcac acgatgcgaa
    10741 acttgctccg gtgttgattc acccagcgcg gaattgtcca tactgatacg aatgtccata
    10801 gtgttctctt tatgaatata cattaaaatg ccgaaccttg atattttttt cgtaaacgcg
    10861 cacgcacgat gatcgccgtc agattgagca aaagcactat cgtgatcaaa agcaacgccg
    10921 tcataaacac catgggtttg gccgcttcta cattgggcga ttggaaaccc acgtcgtaaa
    10981 tatgaaatcc gagatgcatg aatttgcgat ccaaatgaaa gaagggaaac gatccgtcca
    11041 acggcagatt gggagccagc ttaacgacac ccgtgatcat cagcggcgcc acttcgcctg
    11101 caccgcgcgc catagccaaa atcacacccg tcaaaatacc cggtgtagaa gccggtatga
    11161 ccacgcgcca tgtggtttgc cactttgtcg cacccaacgc cagcgacgca tcccgtacgc
    11221 ctttaggaac ggtagtcagg gcttcttccg ttgcaacaat cacgaccggc accgtcaata
    11281 aagaaagtgt cagcgatgcc cacaaaatac cgccggtgcc ccatgtcggc gtcggaaggc
    11341 gatccggata aaaaagttga tctatcgtgc cgccgcagaa gtacacaaaa aaacctaatc
    11401 cgaaaattcc gaaaacgatc gacggtacgc ccgcaagatt attgaccgcg atgcgaatca
    11461 atcgtacagc gattccctgt ttcgcgtatt cgcgcaggta cagcgcggcc acgacgccaa
    11521 atggaactac gaccagattc atcaaaaaca ccatcatcac cgtaccgaat atagccggga
    11581 aaacgccgcc ttccgtattc gactcgcgcg gttcatccgt cagatactca aatattttgc
    11641 ttatataaaa accggatttc tcaaacgtat tcatcgtatt gggttgataa aaacgcacga
    11701 tcttggacac cggtaaatcg cgggtttgac cgtcggtagt gatcacactg acatacatgc
    11761 cgctcgtttt cagatgcaac gtttccaatc gtttttgaat gccctgatac gcttcttgca
    11821 aacgatgaat ctccgcctca agcgacgaca ataattccgg cgtatcttct ttataaaatt
    11881 tcaggcgttt gatttcaagt cgcgtatcct cgatcgcccg gttgatgcga ccaccttccc
    11941 ctttttcgag gtgcagttgt tcatcatgca cgtccaaggc tttgcggaga aactgatcca
    12001 acgtttcggt cgcctgtgct ccgctggcga tgattgttcc gttttctgaa atcgaagcca
    12061 cgcgtccgta gaaattgccg aattcacgcc gttcgataat ggcgaagtca tccggatact
    12121 gcaatgacgc gatatcgtat gtgtttagcc atttgaaatc caacccataa atgtcgcgat
    12181 taccggtttt gacttgaatg cgcgtttgca ctgcttcttt gtacgcgtcc ggcacattgg
    12241 ccagcgggat attttcctcc tgcatgattt cgccggcaaa acgtcgtcca tctttgagtt
    12301 cgacttccgc cacactgccc ggccaaaaaa cgcccacgcc ttgcaagctt acgatgagca
    12361 acaagcccgc aacgagaaac atgatcatac cgagtacggc accggtgaac cacaaaaaaa
    12421 tatcgccgct tttaaaaaga tttttcacgt cattattccg ttacatggat gcatattttt
    12481 ttcgcaaacg ctgacgcact atttccgcca gcgtattgac cacaaacgtg atcacaaata
    12541 acaacaaacc ggataagaag agtacgcggt acagtgttcc accctcaggc gcttctggga
    12601 tttcaacggc gatattggcg gaaagcgtac gcatcccgtt aaaaatgctc cagtccataa
    12661 tgggggtatt gccggtcgcc atgagtacga tcatcgtttc accgaccgca cgaccaaaac
    12721 cgatcatcac ggcggaaaaa atggccgcac tggccgcggg taacaccact ttgatcgccg
    12781 tctgccaacg cgtcgcaccc aatgccaatg aagcgctggt caaatgttta ggtacgctgg
    12841 agagtgcatc ttcggtaatc gtaaaaataa tcggcatcac cgcaaacgcc atcgctatgc
    12901 cgataatgat cgaattacgt tgatcgtagc ggataccgag cgtttgataa agccagtttt
    12961 gcacatcacc gccaagcata ttggtttcaa tccaataacc gaccatgtag ccgatccaaa
    13021 tggaaatcgc caagacaggc aacatcaaaa atacttcata gccgtgacga aacggattcg
    13081 tccatttttt gggcagctta tcaaacaaca atacaaacag gatgatcaac aatggcagaa
    13141 gcgttatcac ggcgataaat tcgatcagca catttttgag caacggcgca agccataaac
    13201 ctgcgataaa cccgatgacg acactgggca atgccgccat aatttccacg gtcggtttga
    13261 cgatcgtacg aatgcgagga tgagcaaatt gcgaggtata caacgcgccc aggatagcca
    13321 atggcgccgc aaaaagcatc gcatacaagg ttgccttgag tgtaccaaaa atcaacggta
    13381 tcagactgaa tttgggttca aaatcatccg tgccgccggt agattgccag gtataacccg
    13441 gcgcatcata actctcgtac catacttcgc cgaagagcgt ttgccaattg ctttccggat
    13501 gcggattatg caatgcaaaa ttttcaactc tgccttcgcc gctcaggaaa actaacccgt
    13561 cggccttggg ggcataactc acctgcgacg gcgcgtacga taacttatgt tctaccagca
    13621 accgatcgct ggttgaatga aacagtttga atacaccgcg gccatcggcg gttacaaaac
    13681 atttattgcg ccaggaagcc gtgatggctt gtatcgaaga ttcgtgccga tcaaaatcac
    13741 ggatgcgtgc aaagttttta ctgccgtcgg tatgcttgac ttccatccat cccgagacgc
    13801 gcccttcttc atctccgacg atcaaactga tatcgccgat cagatacgcc agcgctgtca
    13861 ccgcaattcc gttttgagcg gtagccgcca cggattcgat ccgcgtcggt gcattggttt
    13921 cgcgtatatc ccaacgttcc atgtgcccat cttccaatcc caatacgatt tcttcgcgca
    13981 aaggattgat agccattgag cggatcaccg catcggtcac gacgagtgac ggcaccgtgt
    14041 ctgatgattt gtagtaataa gccgtattct ctttcgccac ggcaacatac gcatccgaag
    14101 ccgttacggc gtaggaaata ccacggaccg gcgttttggt ttttgaaaac gttcgtacgg
    14161 agaggatatg aagttcggga atgatggaac gatgatcacc ggtgaactgc gaagaaaatt
    14221 ttatctccgc ttcgatgata cgaccttgat ccgtcacaca ggcgatacga tgcgatgcaa
    14281 aagcttccat cgcatggatt atgtgttcat ccttatgcaa tgggatgtga aacgtttgca
    14341 gttctttttg atcagaggta cgcatgaaac gcacggcacc gctatcggtg agagcaaata
    14401 gaatttcgcg ttgctcctcc tgaccgatca gcttgattac acttttatca ttggtatcct
    14461 gggctaattc gatcaccgac gctgttgaca attcagcgcc ggaccaaagc ggcaacgcct
    14521 gtttgatgat aaaacccatc atacctaaaa tgatgacgat cagcgcaaat ccgcccgcgg
    14581 caatgatgcc gcttgctaag cgatcgtaaa tatgtcgccg gttcaaaaaa ttcatgattt
    14641 ttccgtttta gattttgcgg gaatcagcag tgacaatatt acggaaaccg aaagtataga
    14701 agcgaccacc gctaaggcga tgacgaccgg tatcttgtaa aaatcaacga tggtcatttt
    14761 cacgccgaca aaaataagca ctaacgccaa accgagtttg agtaagtgaa atttttcaat
    14821 cacactggca agggcaaaat acagcgagcg taaacccaaa atcgcaaaca cattggacgt
    14881 gtacacgatg aaagggtcgg tggtgatcgc aaaaatcgcg ggaatcgaat ccacggcaaa
    14941 aatcaaatcg gtgaattcga caatcaacaa cacgacaaaa agcggcgtgg cccatgtttt
    15001 ggcatctttt tttacaaaaa aacgatcgcc ttcaaaactc ggtgttacgg acatgaattt
    15061 tttaaaaaat cttacaaccg gattggattc cgggtgaatt tccatttctt tctgcacaag
    15121 cattttgata cctgtgatga tcagaaaacc tccgaaaata tatatgatcc aatggaattc
    15181 ggagatcaag gccgcgccga ctgcgatcag aatggcgcgc ataataagcg caccgatgat
    15241 gccccagaac agcactttat gttgatattc ggcgggcacc gcgaagtatg aaaaaatcag
    15301 tacaaaaacg aaaatattat ccacgctcag cgatttttcg atgacgtaac ccgtcaaaaa
    15361 ctgcatcgcg gcttcagaac ccatgtagtg atacacgccg atattaaaaa ccatcgccag
    15421 cgtgatccac acaccgctcc aaatcagtgc ttctttcatg ccgacggaat gggatttctt
    15481 atgaaatatc ccgagatcaa gcgccaacat gagcagaaca aacacattga aaccgatcca
    15541 catcaacact tcgtttgaca ttgtagttcg agctttcggt taggttgtaa tataccgacg
    15601 gaaatctgac atgcgggcag acttccgtcg ggaaaattag gtgcgattat tttttctttt
    15661 caactaattc cagttgttgt ttgaccacat cggcggtcaa cggcatataa ccgtccttga
    15721 ctaccacttc ttgaccttct ttgctaagaa taaatttcaa aaattctttt gtcagcggat
    15781 ccatatcctt ggtgggatgt ttggcaacat acacatacag ataacggctg agcggatatt
    15841 ttccggattt tacattgtcg taggattcgc cgacaaattc cgatttatca tctttggcca
    15901 cgggaatggc gcgcacttcg ctggtagtat aaccgatgcc gctgtaaccg atcgcatatt
    15961 tatcgcttcc cacaccttgc acgaccgaag ccgaacccgg ctgctctttg acttgatctt
    16021 tgaaatcgcc tttgaaaagc gcatgatctt taaaatatcc gtaagtaccg gaagccgaat
    16081 tacggccata caaactgatc ggtttatctt tccattcgcc ttcaaggccg agttgccccc
    16141 aggttaccac atcttctttg gctccgcctt tacgcgtttt tgaaaaaatt gcgtccactt
    16201 gttgcagcgt cagacctttg atagggttgt ctttgtgaac atacaccgct aagccatcat
    16261 acgacgtcgc gatacctgta ggtttgtaac cgtatttttt ttcaaactta tcttcttctt
    16321 cttttttcat cgggcgcgac atcggaccaa gttgggccgt gccttcgatc aaagccggag
    16381 gcgctgtgct ggagcccttg ccttcgatct gcacattgac gctgggataa aatttcttaa
    16441 acgcttccgc ccaaaacgtc atgagattat tcatcgtatc ggaaccgata ctattcagat
    16501 tgcctgaaac accggatgtt ttggcataag ccacaatacc ttcatccact ttgacggctg
    16561 tcgaagcggc aacagctgtt gttgttttgg agggatcggc tgtttcgttt ttgatttctt
    16621 ttttcttatc accgcacgct ccgagcatca tcatcccggc aaaaacagcg gaggctaata
    16681 actgctgcat gtgtttcata ctttctcctg atcgtttatt aaattcatgt ttctgtttcg
    16741 atataataat gcagcaattt atagttgact tgttttctaa agattattca tttgttatca
    16801 aattgttata taaaagacaa aatctatatt gttttattta tacttatata aaaagtagtc
    16861 tatacttatt taagatttat ttatctgaaa taggattttt gtcatattat aagcttaaca
    16921 aatccataat atttgcatca tatcggcgta atgtatcgga attatctttt cgagaatttg
    16981 catgtatttt gaacccgcag ttttcaacca actgctattt aatcaggaga aagaaagaga
    17041 tgaaaaaaat gtttttactg ctcgccttgt gcgtagcgac ggcttcgatt gcgcaggatc
    17101 aaccggccgg caaagtccac ggttatgtat ttggcgatta ttattataag atgggcggcg
    17161 acaaagcaaa agctcaaagc aatacacagt actccgaccc tgcattgcga aaaaaaagcg
    17221 cttttcaatt acgtcgtatg tatctgtatt atgaacatga tttaagttct aatttccacg
    17281 ccacattttt acttgaagga aacgataaag ccatggagtg ggccgaagac acatcaaaag
    17341 ctcaagtgcc tgggcgccat tctgttttta tcaaactcgc taacttagaa tggaaagatt
    17401 atattaaaga tcactccatc gcgatcggtt tgatcccaac agcaacatgg tcgttatctg
    17461 aaaaatattg gggttaccga gcaatcgaaa aaactattac agattatcgt ggattgggtg
    17521 gcgcatccga tattggtatt gtagcgaaag gaaaatttct tgatgatgga atgatcggct
    17581 atactttatc tgtcgtcaac ggaaacggtc aaaaaccgga aaacaataaa tctagaaagt
    17641 atatcggatc actgagtgtt aaaccgatca aatcggttat aacagaaatt tatgctgact
    17701 atgaaggttc catcacggag cccaaaaaat atggcaaagt ggatagccgt aacactctga
    17761 aagcgcttgt tgcatatcag agcgatattg ccaccaccgg tttggaagtt gttcagcaaa
    17821 ctcagaaacg gacggacacg acggacgtaa aaccgtttgg gttctccgta tttgcatctg
    17881 ctccggtgat gaaagaaaaa ctcaatgtgt ttgcacgtta cgattcattt gaccccaata
    17941 cggaaatcac caaagccgga ttcaatgaat atttctttac cgcaggtgtt gactatatgc
    18001 cgatcaaaaa tgttcacttc atgcccaata tttggatcaa cggtttctcg gccaaaaaag
    18061 gcagttcccg tgacgccgat gtggtagcgc gtttgacatt tttttatgtg tataaatagt
    18121 tttttataac gcccttatga agggatgtcc cggattgcga aatcaatttg ggacatccct
    18181 ttttttatta attttttttg agtaaactca tggcgtgaat aatctgtgat ccatatttga
    18241 aagtaagtac ccatgaaaac gaataaaaaa aagcagatcc cgaaaaatta tcagaatttt
    18301 gtcaaagact atccgcgtgt agctaaagcg tatgaggaac ttggcgatgc cgtgcatcat
    18361 gccggacctc tggataccaa aacccgtgcg ctgatcaaat tggctatttc cgcagccagc
    18421 cgtcaggaag gcgccgtaca ttcgcatgtg cgcaaagccc taacagccgg cgcttcaccg
    18481 gaggaaatgc gtcacgtaat catgcttacg cttcccacca tcggatttcc cggcatgatg
    18541 gcggcgatga gctgggtcaa cgacatcatc agttcaaaat agaatttaaa acacctgatt
    18601 tcggtcaatc gccatcctga ctctccgcat tgccatcacg atgctattcg tcgtattatt
    18661 ttttaataga cgaaggaggt ctcatcatga tgcacattca gattgccggc ccgggttgta
    18721 tcaactgtac caaactcgaa gatatgtgcc gtgaagcgat acatgcttat cacgtggatg
    18781 ctcagatcga acgcattacc gaaagacagg actacagccg ccttggcgtt tttgcgacgc
    18841 cgggcttgct catcaatggg cacatcatca gcaccggtcg catgccggct tatcttacag
    18901 tcgaaaaatg gatcaaatct gcggaagatt ggtcttctta aacgattgga taaataattt
    18961 tacttcaagg tttgtgacga cgatcacaaa ccttttttat tttgtattat tttattgtca
    19021 taagggaata gatgacgatt gtcaccgttg tattacacgg cattaacatg aaaggaattt
    19081 tatgagtttt tactctttat tgagtatgtt atgtatcatc ggttgcagta aagaacatga
    19141 taattcaacc gaaattttac gttcaaccat gaggaaggaa cccatgataa caaatcaagc
    19201 ggcggtagat accgcaacgt ttggcgccgg ctgcttttgg tgtgtggaag ccgtgtttca
    19261 gcgaaccaaa ggggtattgt ctgtcaaatc cggttacagc ggcggtcacg tggacaaccc
    19321 gacatatcaa caagtctgca acggcaatac cggccatgcg gaagtcattc aattgacatt
    19381 tgatcctaaa accgtttcgt ttgtcgaatt gcttgaagtg ttttttggca ctcacgatcc
    19441 gaccacactc aaccgtcaag gtgccgatga gggaacacag tatcgctccg ctatttttta
    19501 tcatagtgat gatcagcgcc gcatcgccga ggacgtcaaa caaaaattag atgcatccgg
    19561 tgcattcaaa aaccccattg tcaccgaaat ttcggcgttt aataaatttt atcccgccga
    19621 agattaccat caaaattatt tcaatgataa cggtaatgcg ccgtactgtg cgtacgtgat
    19681 caaaccaaaa ttagaaaaat tcaggaaggt ttttaaagat aagttgaaag cggactgaat
    19741 ctaaataaga aaactatcac ctataaagac tacaaaccat acgatttgcc ataacaaaat
    19801 aaacgctcct gaataggagc gtttattttt atacaacacc ttgtgacgaa gtgcatccgt
    19861 atcatgcgaa aaattcggat aactataaaa aacaaactct cacttcataa aaatcatttt
    19921 gcggctttga atgttattac cggcgcgtag ctggtaaata tatacgccgc tgggtacgag
    19981 cacgccgctg tcatttttac catcccagag tacgcgatga tgaccggaag aacgggtttc
    20041 cgttatcaac cgccttaccg tttgccccaa cgtgttatat atgatcagcg aaacctccga
    20101 tttttcgatc acatcaaacg caagcgtcgt acttggatta aaaggattgg gatatgcttc
    20161 gtatagcgca aaacgtttcg gcgtctgatc aaacggcatc gccggatccc cattttcctg
    20221 ctttttactg attttatcgt tttgagcgat atgctctgtt atgatagatg attcattcgg
    20281 ttcgtttttt tccgtataag cgctttcttc atcctcgatc agatcatcgt tggtacctat
    20341 cgtaaacatg tcatttttct tccaccgaaa gcggtgattc atcatacgaa tagcgtcgcg
    20401 tataggcatc aatattcttc ttttatgatg catagtgagg gtatcggcat tgaccggcca
    20461 ccattctcca tgcgtgacac tatcttcctc agccccggca tcggccatgt cccgcctttg
    20521 attgggatca ttataaccgc cgccacacga caaacaagtg acgcgatatt cattggacca
    20581 gacccctatg gtatcggtca gattacgcat atacacacgg agattaaaat tatacgcgtt
    20641 gctcagtgca ctgatgttaa taaattttga agtcccgctt ccgatatttt gccatacgga
    20701 attaaaatcg tattttaaat accacgtata ttgcagggat gtcccggctg atgccgaaga
    20761 gacggtatat gtaagtcccc cttttttatt aaatccaccc gggccgctga tcgtggccgt
    20821 ctgagagctc atcttatacc gcgcacttat gcttagtgct atatccccga tcaaaacatc
    20881 acgttttacc gcttgcttga tccaatggcg attgcggcgc gtatcccacg ccatcatccc
    20941 acgatctgaa ataagcatac gtaccgccgg taggctctcc gtcagcatcc acctgtgcaa
    21001 ttcggtaagg gagctcgacg gtccgagtat taggccctaa aaaaccaagt ccttcgataa
    21061 gtgatgtatt accggtacgc c
//
LOCUS       DAOQGF010000056        20647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1203_length_20647_cov_7.129322, whole genome shotgun sequence.
ACCESSION   DAOQGF010000056 DAOQGF010000000
VERSION     DAOQGF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1203_length_20647_cov_7.129322"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..3638
                     /locus_tag="PK937_09085"
     CDS             174..3638
                     /locus_tag="PK937_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32765.1"
                     /translation="MHDTDPLYRSLQIPLTRLRREENRLYFQTNALRGAAFLLLLALL
                     TASIEVLSDPDVLGRWVLAMSALMIACVIIAFYIVSPILRLFGVVAASSDFVLAQQIG
                     QRFPEIKDRLLNALQLFGELREGNEKVSEPMIIAAIQTIHTTLEKYDIISVADRTVLR
                     KTWRYTSVVVIAASIVFLFFNRPLTDAFARLADPGKKPMEVIPYRWVIVPGTTDILQG
                     QNRKISVEIIPLTSDAAMPDAVQLLTQLSGVEQVTETLMKKDTARFYSLELQNLRQET
                     TYWFKFSYVENSRKRAAISEKNTLHVMRRPSVKRLQIEIESPTYARIEHRILEANSGD
                     IQALKGSRVRVTMEATKSLQEARIVFSDSTSIPLKTSVFNYEKAEGSFKVMKSGTYHF
                     VMTDKEGTPSADPVEYRIDVQDDLYPLVQLNIPEKDGDITEAMLQKIVADVQDDFGVS
                     RIVLNYKLMHTRGVLPIPETYTTEDLSFLIRSDQSEFDVAYNWEFSKLLLQPEDVIGF
                     YLEVWDNDAVSGPKKTKSVERRLRFPSMEEIFAETNQKQDQQIQAAEEIAKESEEIKK
                     SLEEVHREMLKDKKPEWKDKEKIKQLVEKQEELQKKAESIQKSLEEMTDKLKEQNLLT
                     PETVQKYQELQKLLSEINSKEMQELMQKMQKAMENNFDPNQMRDALKNFKMDQEAFKK
                     NVDRTVELLKRIKAEQMFDQLRRTAEDLEKQQKDLDRMTSNLKNDNERTAAIKEQERI
                     RKNTDDLKKKSDELQKLVKELNNNQSNQKLNTAQDQLQQNNIQNRMQQSQQAMSDKQE
                     GTKEEKENREDIHKSLDSLSRSMADAQKEHRANQDEAIKRAMQKIVVDMLVISQNQEA
                     LISETSPLNFASVRFREVTQDQSDILSNMGIVTENLLELSNKTFFVNTPLGKTVGQAM
                     GQMQTAVKAMEERNTPQAVNSQTAAMVSVNEAIKLLLLSMDKMNNGQSGTGMEELMEQ
                     MREAAGKQRRINQGMPSLMEGQDNNPGGLSQEQQAQLGRMMAEQQALKQSMEAMKDQA
                     GAQQGLKGQLGNLAKEMEEVIKDMQSNQVNRKTIERQQKILQRMLDASTSMQEKDMSE
                     KRKGETGKNYDAVSPRALPENLTDRRNFLRQELLRQMKQGYAKDYQELIKRYFDELGE
                     KTGLPKND"
     gene            complement(3635..5281)
                     /gene="hutU"
                     /locus_tag="PK937_09090"
     CDS             complement(3635..5281)
                     /gene="hutU"
                     /locus_tag="PK937_09090"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNH32766.1"
                     /translation="MRTIRAPRGTSITCKNWLTEAPLRMLMNNLDPDVAERPEDLVVY
                     GGIGKAARNWDCFDAIVKSLTNLAMDETLLVQSGKPVAVFKTHADAPRVIIANSNIVP
                     HWATWENFREYEKLGLIMYGQMTAGSWIYIGTQGILQGTYETFAECGRKHFKGTLRGT
                     ITLTAGLGGMGGAQPLAVTMNDGVAICIEVDRTRIQRRLDTRYCDVMVESLDEAWRMA
                     TECKAKGEAKSIGLLGNAAELLPEMLKRNMIPDIVTDQTSAHDELNGYIPTGISVEEA
                     TEWRKNRTQEYIGRAMESMAIHVRAMLDFQSRGAIVFDYGNNIRGQALKVGVTNAFDF
                     PGFVPAFIRPLFCEGQGPFRWAALSGDPEDIYVTDKAIMEAFPEKAGLQRWLSQAKDR
                     IAFQGLPSRICWLGYGEREKAGLIFNELVRTKKVKAPIVIGRDHLDCGSVASPNRETE
                     AMKDGSDAIADWPLLNFAINAVNGATWVSLHHGGGVGIGYSLHAGMVIVADGSPEAAV
                     RLKRVLTCDPGMGVVRHVDAGYDEAIKVAKEKNVHIPMMR"
     gene            complement(5306..6289)
                     /locus_tag="PK937_09095"
     CDS             complement(5306..6289)
                     /locus_tag="PK937_09095"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNH32767.1"
                     /translation="MVNETAFTRHAGVQYPILCGAMYPCSNPELVAAVSAAGGLGIIQ
                     PLSLTYVHKYDFRQGLRYIRSLTDKPIALNATVEKSSRVYEDRLRQWIDIALEEGIRF
                     FDTSLGNPRWVVDRVAPYQGIVYHKVTERKWAEKAMDAGVHGLIAVNARAGGHAGHLS
                     PQQLYDALADTGLPIVCAGGIGDENDYADALKIGYAGVMMATRFIATTECTAHEDYKR
                     AIVQAGEADIVHTERVTGVPLAVIKTEYVERVGYKTGTIARLMLKHRQLKKMMRLIYA
                     LRSMRQLKRSSLQGKMSTRDYYQAGKSVTGVHAVESVAAIIARFVRSTKPK"
     gene            complement(6279..7211)
                     /locus_tag="PK937_09100"
     CDS             complement(6279..7211)
                     /locus_tag="PK937_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNH32768.1"
                     /translation="MRFASIDIGTNTVLLLIAEADSSGHLHTLFEKQRIIRLGKNVDA
                     QKNIAHEGMDKLTHVLTEYLQDAANHQTAKIVIGATSAMRDAANKETILNEIYHRTGQ
                     RIQILTGDEEAQWTFQGGCLALPPLTRHPILLIDIGGGSTEFVVGSASDMTFKKSLNI
                     GAVRMTERFINHDPPLQSELESLRRYAHNEFDTHLSGIKNIQPAHALGVAGTVTTLCA
                     MLQALPHYDPKRINGYMIDKAALDSLIEKLSDLTLEERRKIPGLEPERADVIIAGATI
                     LSEIFSFFNLDKITVSNFGLRYGLILRELANHGQ"
     gene            complement(7211..8113)
                     /gene="prmA"
                     /locus_tag="PK937_09105"
     CDS             complement(7211..8113)
                     /gene="prmA"
                     /locus_tag="PK937_09105"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNH32769.1"
                     /translation="MQPKKKKSRSSTRTKKKKDFWWEVDLGHIVNLEATQTLLFELGS
                     TGSIETATGLTAYFQPADYSSEKILRAKLAPRLGPGTTVHIRPMAAQDWESEWKKNFK
                     PRRVGKHFIVRPSWEKYKRKLKDIILVIDPKMSFGTGTHETTQLVLGFIESFSVKNKI
                     LLDVGSGTGILAIAAAKSGARAVYGFDVDTNAFENAEENIRRNRVSARVKTVLTTIET
                     LPSKWPERYDIILANIQRAVFEMPGFMENLNQKMKPTGMMILSGILAIEDERMRMLFS
                     EHNLHVIEFRADGEWVAYVLQRTS"
     gene            complement(8138..9199)
                     /locus_tag="PK937_09110"
     CDS             complement(8138..9199)
                     /locus_tag="PK937_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32770.1"
                     /translation="MKDHILVVDDELDAVDPLIRVLQHDGYEVDYAANAKEALGYVKT
                     TSYQLILMDVMMPGMSGYQLLKKLQEDEETAYIPVIFVTGHFNAEEIVKGLEAGAVDA
                     ISKPFHIAEVITRGKVRIAESKLKRRYTPIHHFFAEAQEKEQSRRTGCFEFFDKSKNK
                     VGDVFVSEGKIVYATSKNAIKEDAFLQLASMKDITYIFQEGVRSQNTSFSASVTSLIL
                     EASKIVDELEAKEIRDEDQKRVLVIDRDRIPRILASRALKSAGYAPTVTSPDEVTQDI
                     ISKYDPHVVIVDPQDCELVLNKVALTRPSRQPIPLIVYGDQDQLEKHDALRKYHIKAF
                     VDKAQTNESLAHAVFQALK"
     gene            complement(9237..9971)
                     /locus_tag="PK937_09115"
     CDS             complement(9237..9971)
                     /locus_tag="PK937_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32771.1"
                     /translation="MAETKKTRSKLLLVDADEQARLLIKENLIKEDYIVFDTGNGNVA
                     LEIVNREKPDLVISDIFLGEMNGFELCQRIRDTSNVPTVPFVFFTAIDDMLTEIRGFR
                     CGANDYLVKRHTKRQDLLLRIESMVSQVNAFEKEKATLRDGFLGKLGDVSVIDVLQLL
                     ANTAKTGNLLISDGTIEGNVYFMKGQIFNATLGHVQGEDAIYDMIYWEDGFFRFTQEE
                     IPRQSVIASPTNKIISKCRRLLGKEE"
     gene            complement(9992..10714)
                     /locus_tag="PK937_09120"
     CDS             complement(9992..10714)
                     /locus_tag="PK937_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32772.1"
                     /translation="MPKKILIVDDNKTVRTLLSINLRKENFEVIEAENGAEGLQMVND
                     HLPDLVISDIIMPQMDGFEFCRKVRETSKSPMVPFMFLTSIDQVATELRGLRTGADDY
                     LIKSNIKKEELVGKVSAMLQKSNEYKQAENQMQEGLYGKFSDLSLVDVFQLLIMNKKT
                     GLLTITRDSDKAEITFEDGRMLHAEYKQFVGEEAVYNLGEWKTGVFKFESVEPKVTPT
                     IHSATMNILMEYCRITDENKLN"
     gene            complement(10748..11971)
                     /locus_tag="PK937_09125"
     CDS             complement(10748..11971)
                     /locus_tag="PK937_09125"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNH32773.1"
                     /translation="MMRGFEKAADILELDTGVRQYLLTPEKQVTVSIPVVLEDGSIQV
                     FKGFRVIHSTVRGPSKGGIRVSPKATLDETTALAAWMTWKCAVVNIPFGGAKGAIECD
                     PSRLTESAYEKIIRRYTANLFEIFGPDKDVPAPDNNTGEREMGWIMDTYSMHVRRTET
                     AVVTGKPLIMGGSHGRRDATGRGIMLIALAAMKQRNIPANEARIVIQGFGSVGATAAQ
                     LLYENGCKIIGIGDASGSYFSEKGINISDAIRFAQQNRRSLSGYPNAQKITNEELLML
                     DCDVLIPAAMENQIHAENASKIQAKIICEGANGPITPEADTLLHERGILVVPDILANA
                     GGVTVSYFEWVQNRMGFFWPLHEVHDRLERVMIESFESVHAVALRHTQSMRIGAYILA
                     LDRVTQVLKLRGIYA"
     gene            complement(12149..13090)
                     /locus_tag="PK937_09130"
     CDS             complement(12149..13090)
                     /locus_tag="PK937_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32774.1"
                     /translation="MAIGLYCSGFPLYTQDRYDPEINRRPYVPDVTKHSANTLDSITA
                     ADYAKRNKIKSNMADYKPSYWHINFIFGASYLHERNNIEQFYDNEGYVYHVADLKDPD
                     EKKHNPKLIQISVQHNLSPHWQIEAVVSRIFNQTLAGSNWLQFVNEEDTPPSQRFDLE
                     QKSEIKMLQVGGRYIFMPVNMTTRLELSTGAGIAYYSYHTSSHHYYAQNIGYHSHPQL
                     INQTSSSFSYDRNALGAVADLSLDFYLSPKISCQFRYEKRITEKIKLPSESFEYVIRK
                     QIGPREFIMETHEKHIPSRTIDLSGYTLNMALHLHLF"
     gene            complement(13187..13810)
                     /locus_tag="PK937_09135"
     CDS             complement(13187..13810)
                     /locus_tag="PK937_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH32775.1"
                     /translation="MSKKPITNKDVTPRMFESDFLEFFSKVHFSVPLIVFIPIISYFI
                     YQSLWIEKSDIKHFLFWILAGLAVWTLTEYVLHRFIFHFTPRSALMERIHFIFHGVHH
                     DYPNDSKRLVMPPAASIPLAALFYFLFLIIFGSNIAPMFAGFLIGYLWYDMTHYAIHH
                     FNMHSAFWLAIKNHHMKHHFQNPEKGFGVSTPIWDYVWQTMFPAKNK"
     gene            14041..14541
                     /locus_tag="PK937_09140"
     CDS             14041..14541
                     /locus_tag="PK937_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNH32776.1"
                     /translation="MFETHIIEYSAKSEMYAALHAQIHHLLDGETDLIANAANASSLL
                     FHTLPALNWAGFYFYKGNELIVGPFQGKPACVHIAMGKGVCGTAAQQRKTVVVKDVHT
                     FPGHIACDSASNSEIVVPLISGGQLFGVLDLDSPQMARFDEADQAGLEEFVDIFMKHT
                     TWPKNL"
     gene            complement(14536..15075)
                     /locus_tag="PK937_09145"
     CDS             complement(14536..15075)
                     /locus_tag="PK937_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32777.1"
                     /translation="MTKSEIIKMLTGNDRRTIAHANLLVSKLTHDRTGLKNLYHLLSH
                     EDADICFRASDTLEKILRNETAAIRTLSPYLNKDWPKLIGNEALQMHLPLILGYVAWK
                     KNDATRIGTRLLELAHTTKNKFVTVNTMTGLADIALRHTWLVPTVWGLLDEMEMKGSA
                     ALRARARILKKKINLATKL"
     gene            complement(15068..16171)
                     /locus_tag="PK937_09150"
     CDS             complement(15068..16171)
                     /locus_tag="PK937_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNH32778.1"
                     /translation="MAEPLKNMFSGSFVETLGDQIQTVYSSFDKRAFTNFIFDHAWSQ
                     RELKDRMHHISSALRSHLPEDYRKALGILIHVTKHFRMNDTKISFTDMVFPDFVEHYG
                     LDDLESSIPALELFTQVSSAEFAVRPFILRYPDIMLQQMLVWSRHSSPHVRRLASEGC
                     RPRLPWAIALPVFQKDPAPILPILENLKADPSEYVRRSVANNLNDITKDNPEIVLQIA
                     ERWRGLSPETDKLVKHACRTLLKRGNKKALTIFGTHDASGVSVLSFVSKKKKIFIGDT
                     LDFETEIRIRKAKTLRIEYAIDFVKSGGKISRKVFKWREGLYDSGSLKLSKKHRFQNF
                     TTRKHYSGKHAITLIINGHPIADLDITLGDSHD"
     gene            complement(16175..16552)
                     /locus_tag="PK937_09155"
     CDS             complement(16175..16552)
                     /locus_tag="PK937_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH32779.1"
                     /translation="MNTKILGLCSVVQHVTDLEAAKRWYTDAFGVKPYFDQPFYVGFQ
                     FGGFELGLDPDPKQYNSPGFGSITYWRTDNVQAMFDHLIRSGAEANEHPNCVGGDLII
                     ASVRDPFGNVIGLMYNPEFKITA"
     gene            complement(16555..17085)
                     /locus_tag="PK937_09160"
     CDS             complement(16555..17085)
                     /locus_tag="PK937_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH32780.1"
                     /translation="MNITAHLHQFESIANDWMMALDTYDERKFNRAPDQESWSIGQVY
                     YHLVHGTQNFHLRMVRQCMDNPDHAHEKKTFPGKIVFFTGRFPPIRIKVPPSDMYTPK
                     QPESIAAMRRDLNTLIGTMRTVASELSQKRNQGKAQHPRFGYLSGDEWFAMIQMHFRH
                     HLYQKNRLDNFLKKGA"
     gene            complement(17125..17397)
                     /locus_tag="PK937_09165"
     CDS             complement(17125..17397)
                     /locus_tag="PK937_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNH32781.1"
                     /translation="MAVIKIKPEFVSRMPDIVAEAKSIIGTYEGFISWENMRADDTSR
                     VFVDILRWKTKDHADQAFEKFHSTESCSRLMNALEKDVYFGHVRHY"
     gene            17668..19053
                     /locus_tag="PK937_09170"
     CDS             17668..19053
                     /locus_tag="PK937_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNH32782.1"
                     /translation="MTFSQSMHSDKMRVALTSVLAAIFLTGMKLAAGLATGSLGILSE
                     AAHSALDLGAALLTYVAVRISDKPADDDHSYGHGKVENISALLQTGLLILTCFFIIRA
                     AVERLMSGDVQVEVNVWSFVVIIVSIIVDYSRSRALARIAKTYRSQALEADALHFSSD
                     IWSSSVVIIGLISTALGFHQGDSLAAIGVALFVLVISGKLLKETIDALTDSMPKDIEQ
                     RIRTALVSLDGVERYRHLRVRQSGSKVFVDMYIDVKRTVPFEMAHHVADAVETRIHEI
                     VPNADVIIHMEPQETDDESIIDKIRMIVVQEGLACHNVRAQRVTNGYFVDFHLECESH
                     QRFDEAHEASSRIESRLMEKIPEIKDVKVHIEDARDRVVQAFDITDQSKPLIAAVRAI
                     AANTQPVRACSDFIVMDIGGHKKLMLNCVVPGDLSLDEVHAITTRLENRIHRDIPEVS
                     SIVIHPELARA"
     gene            19046..19819
                     /gene="pgeF"
                     /locus_tag="PK937_09175"
     CDS             19046..19819
                     /gene="pgeF"
                     /locus_tag="PK937_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNH32783.1"
                     /translation="MPEPSMIFEEKDGLRLCRFPALEKFPDVMAVMSTRIGGVSEGDY
                     KGLNLGLSTDDNPHHVEENRQRFWRALGVDMNDVIRQHQVHEDRVVFLQEQTLCADTD
                     AMYTDQSKKFLTVVAADCVPVLFYEPAHRIIAVIHAGWRGTLKQIIKKTMASIQQTFG
                     CSLGHAVAVLGPSIGADHYEVSDEVASMFDTAFVNRRDYPKPHLDLWKANAAQLDEAG
                     VGHIVVTGACTMCQPDIYYSHRASGGRSGRMVGVIGRRN"
     gene            19824..20546
                     /locus_tag="PK937_09180"
     CDS             19824..20546
                     /locus_tag="PK937_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH32784.1"
                     /translation="MDHIEKYFGSLIQRAGDAIFTVSPSQIILSWNDGARELLGYDKE
                     DVLGHSVDVLSPLDDKRHMRAMAIEVFDHGALKNLETELTRKDGHVVSAYVTVSPVTD
                     ENENVVAVSFIAKDITDQNRLLIHLIESQRQAGQIQALVEALTTLSHHIRNASAVISA
                     KSEVSLELNQLAVYHDLVRVCTHETRRITAVIDTLQDLVREMQVSGEPIDTVQMNGAP
                     APLLDIEARLKERLKKLDERPK"
BASE COUNT         5658 a         4492 c         4806 g         5691 t
ORIGIN      
        1 cgttcggcgg gttcaacatg agcggtaccg actccaaagc gggtggacgg gattacctga
       61 tgctatttac acaagctaaa gtcgtcagtg aaaaattaaa ataatgcaac agtttttgaa
      121 cggattcgta aaaaaccgat aggtattatc ctgtcggttt tttattttac tatatgcacg
      181 atacagatcc cttatacagg tcattacaga ttccactaac gcggcttcgt cgtgaagaga
      241 atcgtcttta ttttcaaacg aatgcccttc gcggcgcggc ttttttgctg ttacttgctc
      301 tcttgacggc aagtattgaa gtactttctg atccggatgt gttagggcga tgggtattgg
      361 cgatgagcgc acttatgatc gcctgtgtga taattgcttt ctatatcgtt tcgccgattt
      421 tgcgtttgtt cggtgttgtt gcggcttcat cagattttgt tttagcgcag cagatcggtc
      481 aacgttttcc ggaaattaaa gatcgtcttt tgaatgcgtt gcagcttttt ggggaattgc
      541 gcgaagggaa cgaaaaagtt tccgagccca tgatcatcgc ggcgatacaa acaatacata
      601 ctacacttga aaagtatgat atcatcagcg tagccgatcg tacagtactt cgtaaaacat
      661 ggcgatatac gtctgtcgtt gttattgcgg caagcattgt ttttttgttt tttaatcgcc
      721 ctttgactga cgcatttgcg cggctagccg atccgggaaa aaaaccgatg gaggtcattc
      781 cgtaccgatg ggtgatagta ccgggaacga ccgatatact ccaaggtcag aatcgtaaaa
      841 ttagcgtcga aattattccg ctgacttccg acgcggcgat gccggatgcc gtgcaactct
      901 taacacagtt gtccggagta gaacaagtga ccgaaaccct catgaaaaaa gatacggcac
      961 ggttttactc tttggagtta caaaacctgc gtcaggaaac gacatactgg tttaaatttt
     1021 catatgtaga aaatagccgc aaacgtgctg caatttcaga aaaaaataca cttcatgtga
     1081 tgcgtcgtcc gtcggtaaaa cgccttcaga tcgaaatcga atccccgact tatgcgcgca
     1141 tcgaacaccg tatcttagaa gccaatagtg gtgatataca agccctcaaa gggtcccgtg
     1201 tccgggttac catggaagcg acaaaatcac tgcaagaagc gcgtattgtc ttttccgatt
     1261 cgacttcaat accgctgaaa acttcggtgt ttaattatga aaaagccgaa ggttctttta
     1321 aagtaatgaa gtcgggaacc tatcattttg tgatgacgga taaagaagga acacccagtg
     1381 ccgatccggt cgaatatcgc atagatgtgc aagacgattt atatccgttg gttcaattga
     1441 atattcccga aaaggacggc gatatcaccg aagctatgct acagaagatc gtagccgatg
     1501 tgcaggatga ctttggtgtt agccgaattg tgctaaacta taaattgatg catacccgcg
     1561 gtgttctgcc cattccggaa acatatacca cggaagattt aagttttttg attcgtagcg
     1621 atcagagcga atttgatgtc gcttataatt gggaattctc aaaactgcta ttacaaccgg
     1681 aagatgtgat cggcttttat cttgaggtgt gggataatga tgccgtttcc ggaccaaaaa
     1741 aaacaaaaag tgtcgagcgt cgtctgcggt ttccttcgat ggaagaaatt tttgcagaaa
     1801 ccaatcaaaa acaagatcaa cagatccaag cggctgagga aattgccaaa gaaagtgaag
     1861 aaataaaaaa atcgctggaa gaagtacatc gtgagatgct caaagataaa aaaccggagt
     1921 ggaaggacaa ggaaaagata aaacagcttg tcgaaaaaca agaggagctt caaaagaaag
     1981 cggagtccat tcaaaaaagc ttagaagaaa tgacggataa acttaaagaa caaaatctgc
     2041 ttacacccga aacggttcaa aaatatcagg aattgcaaaa actacttagc gagattaatt
     2101 ccaaagaaat gcaggaattg atgcaaaaaa tgcagaaggc catggaaaat aattttgacc
     2161 ctaaccaaat gcgggatgca cttaagaatt ttaaaatgga tcaagaggcg ttcaaaaaaa
     2221 atgtggatcg gactgtcgaa ttgctcaaac gtatcaaagc cgaacagatg tttgatcagt
     2281 tacgccgcac agcggaggac ttggaaaagc aacaaaaaga cctggatcgg atgacgtcga
     2341 acttaaaaaa cgataatgaa cgtaccgccg caatcaaaga acaagaacgc atccggaaaa
     2401 acaccgatga tctgaaaaaa aaatcggatg agttacaaaa attggtaaaa gaattaaata
     2461 ataatcaatc caatcaaaaa ctcaatacgg cgcaagatca gttgcaacaa aacaacattc
     2521 agaaccgcat gcaacagtca cagcaggcca tgagcgataa acaagaagga acgaaagaag
     2581 aaaaagaaaa cagagaggat attcataaat ctttagattc gctgtcccga tctatggctg
     2641 atgcacaaaa ggaacatcgc gccaatcagg atgaagctat aaaacgtgcc atgcaaaaga
     2701 tcgtcgtaga tatgctggtc atttcacaaa atcaggaggc tttgatttcc gagacttcgc
     2761 cgttaaattt tgccagcgtt cgttttcgtg aagtgacaca agatcagtca gacattttat
     2821 ccaatatggg tatcgttacg gaaaatctct tggagctttc caataaaacc ttttttgtta
     2881 atacgccgct gggcaaaacc gtcggccagg ccatgggaca aatgcaaacc gccgtgaagg
     2941 ccatggaaga acgtaatacg ccacaagctg tcaattcaca aacagcggct atggtatccg
     3001 tcaatgaagc aatcaagtta ttgcttctga gtatggataa gatgaataac ggccaatcgg
     3061 gtacaggaat ggaagaactg atggaacaga tgcgtgaggc ggccggaaaa caaaggagga
     3121 ttaatcaggg aatgccatcg ttgatggagg gccaagataa taacccgggc ggactatcgc
     3181 aagaacagca agctcaactc ggtcgcatga tggctgagca acaagcactc aaacaatcta
     3241 tggaagccat gaaagatcaa gccggcgctc agcaaggctt aaaagggcag ttgggtaatt
     3301 tggctaaaga gatggaagag gttatcaaag acatgcagtc caatcaagtt aatcgtaaga
     3361 ccatcgaacg gcagcagaaa atacttcagc gtatgttgga tgcttcgact tccatgcaag
     3421 aaaaagatat gagcgaaaag cgaaaaggcg aaaccggaaa aaattatgat gcggtttctc
     3481 cgcgcgcatt gcctgaaaat ctgacggatc gtcgcaattt tttacgccag gaattattgc
     3541 gccaaatgaa acaaggttat gctaaagatt atcaggagct gatcaaacgt tatttcgacg
     3601 aacttggcga aaaaaccggt ttaccaaaaa acgattagcg catcatcgga atatgtacgt
     3661 tcttttcttt ggctactttg atggcttcat cgtaacccgc gtccacatgt cggacaacgc
     3721 ccataccggg atcacacgtt aacacccgtt ttaaccgcac agccgcttcc ggcgaaccat
     3781 cggcgacgat caccatacct gcatgtaaac tgtatccgat acccacaccg ccgccgtgat
     3841 gcagcgatac ccacgtcgcg ccattgaccg cgttgatcgc aaaatttaac aacggccagt
     3901 cggcgatagc atcagagccg tctttcatgg cttcggtttc tctgttaggt gaagcgacag
     3961 aaccgcaatc taagtgatca cgaccgatga cgatgggtgc ttttaccttt ttggtacgga
     4021 ccaattcgtt gaaaatcaaa ccggcttttt cacgttctcc gtaaccaagc caacaaatgc
     4081 gactggggag gccttgaaaa gctatacgat ctttggcttg cgacaaccaa cgttgaagac
     4141 ctgccttttc aggaaaagcc tccataatag ctttgtccgt tacataaata tcttccgggt
     4201 caccggagag cgcagcccaa cgaaagggac cttgcccttc gcagaataac ggacgaataa
     4261 atgccggaac aaagcccgga aaatcaaacg cattggtcac gccgacttta agcgcttgcc
     4321 cgcgaatatt attgccgtag tcaaatacga tcgctccacg actttgaaaa tcaagcatag
     4381 cgcgcacatg aatggccatc gattccatgg cccggccaat atattcttgg gtgcgatttt
     4441 tacgccattc ggtggcttct tcgacggaga tacccgtagg aatatagccg tttaattcgt
     4501 catgtgccga cgtttgatcg gttacaatgt ccgggatcat attccgtttg agcatttctg
     4561 gtaaaagttc tgcggcattt cccaataagc ctatggattt ggcttcgccc ttggctttac
     4621 actccgtagc catacgccag gcttcatcca gcgactcgac catgacatca caataacgcg
     4681 tatccaaacg acgttggata cgggtgcgat caacttcaat acaaatagcc acaccgtcat
     4741 tcattgtgac agccaatggc tgtgcaccac ccataccgcc caatccggcc gtcagcgtga
     4801 tcgtaccgcg caaagtgcct ttaaaatgtt tgcgcccgca ctcggcaaaa gtttcatacg
     4861 taccttgcaa aatgccttga gtaccaatat aaatccagct tccggcagtc atctgtccat
     4921 acatgatcaa gcctaatttt tcatattcgc gaaaattttc ccatgttgcc caatgcggaa
     4981 cgatattgga attggctatg ataacgcgcg gcgcgtcggc atgggtttta aaaacggcga
     5041 ccggttttcc ggattgaacg agcagagttt catccattgc caaattagta aggcttttta
     5101 cgatcgcgtc aaaacaatcc caattgcggg ctgctttgcc gatgccacca tacaccacaa
     5161 gatcttccgg acgttccgct acgtcgggat caagattatt catgagcatg cgtaaaggcg
     5221 cttcggtaag ccagttttta caggtaattg aagtgccgcg gggtgcgcgt atggttcgca
     5281 tagatgaatt cctttatgat tacaattatt ttggttttgt agatcgaaca aaacgtgcga
     5341 tgatggccgc cacggattcg acggcatgaa caccggtgac gcttttaccg gcttgataat
     5401 aatcgcgagt ggacatcttg ccttgcaatg atgagcgctt taactggcgc atggaacgca
     5461 acgcgtaaat caaacgcatc atttttttta attggcgatg tttgagcatc aggcgcgcta
     5521 tggtgcccgt tttgtacccg acgcgctcga catattcggt ttttatcaca gccaatggta
     5581 cgcctgtaac gcgttccgta tgaacgatat ctgcttcgcc ggcttgtacg atggctcgtt
     5641 tgtaatcctc gtgtgccgta cattcggtcg tagcaataaa tcgcgtagcc atcatgacgc
     5701 ctgcataacc gattttcaag gcgtctgcat aatcgttttc atcaccgata ccaccggcgc
     5761 acacaatcgg taaaccggta tcggccagcg catcatatag ttgttgcggc gagagatggc
     5821 cggcatgtcc ccccgcgcgg gcattgacgg ctatgagacc gtgcactccg gcatccatcg
     5881 ctttttccgc ccatttgcgt tcggtgactt tatgatagac gatgccctgg tatggtgcga
     5941 cacgatctac gacccagcgc ggattgccaa gcgatgtgtc aaaaaaacgg ataccttctt
     6001 ccagcgcgat atcaatccac tgccgcaaac gatcttcata aacgcgcgac gacttttcga
     6061 ctgtcgcgtt gagtgctata ggcttatccg tgagagaacg aatataacga aggccttggc
     6121 ggaagtcata tttatgaacg taggtaagtg ataagggttg gatgatgccg agtcctccag
     6181 ccgcagaaac agcggcaaca agctccggat tgctgcacgg atacatagcc ccgcaaagaa
     6241 tagggtattg cacgccggca tgacgcgtaa atgcagtttc attgaccatg atttgcaagc
     6301 tctcttagta tgagaccgta acgtaagcca aaattgctga ccgtgatttt atctaaatta
     6361 aaaaaagaaa atatttccga taaaatcgtt gctcccgcaa taataacatc cgcacgctcc
     6421 ggttccaatc ccggtatctt acgacgttct tcgagggtaa ggtcgctcag tttttcgatc
     6481 agcgaatcca acgccgcttt atcaatcata taaccattga tgcgcttagg atcataatgc
     6541 gggagcgctt gcaacatcgc acataatgta gtaacggtac cagcaacacc gagtgcatgg
     6601 gccggttgaa tattttttat accggataaa tgagtgtcaa attcgttgtg agcataccgg
     6661 cgaagacttt ccaattcaga ttggagaggc ggatcatgat tgataaaacg ctccgtcata
     6721 cgaaccgcgc caatgttcaa actttttttg aacgtcatat cggaagcgga gccgacaaca
     6781 aactcagtac tcccgccgcc tatatcaatc aagagaatgg ggtggcgcgt caatggggga
     6841 agagcaaggc aaccgccttg aaacgtccac tgggcttctt catcaccggt taaaatttga
     6901 atacgttgac cggtacgatg ataaatttca tttaaaattg tttctttgtt tgcggcgtcg
     6961 cgcatggcgc tggtggcgcc gatgacgatc tttgcggtct gatgatttgc ggcgtcttgc
     7021 aaatattcgg ttaaaacatg tgtgagttta tccattccct cgtgggcgat atttttttgt
     7081 gcgtccacgt tttttccgag tctgatgata cgttgctttt caaagagcgt gtgcaaatga
     7141 ccgctggaat ccgcttctgc gatgagtaaa agtaccgtat tggtgccgat gtctattgag
     7201 gcgaaccgca tcacgatgtc ctttgcagta cgtaagcgac ccattcaccg tcggctcgaa
     7261 attcaatgac atgcagattg tgttcagaaa acaacatgcg catacgttcg tcttcgatgg
     7321 cgagaattcc agaaagaatc atcattccgg tgggtttcat tttttgattc aaattttcca
     7381 taaacccggg catctcgaat accgcgcgtt ggatattggc tagtattata tcgtaacgtt
     7441 cgggccattt agagggcagg gtttcgatag tggttagtac cgtttttaca cgggctgaaa
     7501 cgcgattacg acgaatgttt tcttcggcat tttcaaaggc atttgtatcc acatcaaagc
     7561 catacacagc gcgtgctccg gacttggcgg cggcgatcgc cagtatgccc gtcccactgc
     7621 cgacatctag cagtatttta ttcttaacgg aaaacgattc gataaaacct aaaactaact
     7681 gagttgtttc atgtgttccc gtaccaaatg acattttagg atcaataacc aaaatgatgt
     7741 ctttgagctt acgtttgtat ttttcccatg aggggcgcac gataaagtgt tttccaacac
     7801 gtcgcggttt gaaatttttc ttccattcgc tttcccaatc ttgagcggcc attgggcgaa
     7861 tgtgaaccgt cgtgcccgga ccaaggcgcg gtgcgagttt ggcgcgaagt attttttctg
     7921 aggaataatc ggctggttga aaataggccg tgagccccgt tgccgtttcg atggagccgg
     7981 tcgaacccaa ttcgaataat aaagtttgtg tagcttcgag attaacgata tgaccgagat
     8041 caacttccca ccagaagtct ttttttttct tagtacgtgt gctggaacgt gatttttttt
     8101 tcttgggttg catgattcac ggcggaaccg cccgatatta tttcagcgcc tgaaaaacgg
     8161 catgggcaag cgattcatta gtttgtgcct tgtcaacaaa tgccttgata tggtatttgc
     8221 gaagcgcgtc gtgtttttct aactgatcct gatcgccgta cacgatgagc ggaatgggtt
     8281 ggcgggatgg cctggtgagg gctactttgt taaggactaa ttcacaatct tgcggatcta
     8341 cgatcaccac atgcggatcg tatttggaaa taatgtcttg tgtcacttca tccgggctcg
     8401 ttacggtcgg agcatagccg gccgatttaa gagcgcggct cgcaagaatt cggggaatac
     8461 ggtcgcggtc aatcacgaga acacgttttt gatcttcatc gcgtatttct ttggcttcca
     8521 attcatcaac gatcttggag gcttctaaga tgaggctcgt tacgctagcc gaaaaactgg
     8581 tattttgaga gcgtacacct tcctgaaaaa tataagtgat atctttcatc gatgcgagtt
     8641 gaagaaaagc gtcttctttg atggcatttt tgcttgtcgc gtaaacgatc ttgccttcgc
     8701 tgacaaatac gtcgccgact ttatttttgg atttatcaaa aaattcgaaa cagcccgtgc
     8761 gacggctttg ttctttttcc tgtgcttcgg caaagaaatg gtgaatgggt gtataacggc
     8821 gtttgagttt ggactcggcg atgcgcactt ttccgcgtgt gataacctct gcgatgtgaa
     8881 aaggtttgct tatggcatca acggcgccgg cttccagtcc tttgacgatt tcctccgcat
     8941 taaaatggcc ggtaacgaaa atgaccggta tatacgctgt ttcctcgtct tcttgaagtt
     9001 ttttcaaaag ttggtaaccg ctcatgccgg gcatcatgac atccatcagg atgagctgat
     9061 acgatgtcgt ttttacatag cccaatgctt cttttgcatt ggctgcgtaa tcaacttcat
     9121 aaccgtcatg ctggagtacg cgaataagcg ggtcaacggc gtccagttcg tcgtcaacca
     9181 cgaggatgtg atctttcatg gcagtctatg gtgtataagt aaaatatatt tgaaaactat
     9241 tcttctttcc caagtaaccg tctgcattta ctgatgattt tattggtcgg cgatgcgatg
     9301 acgctttggc gcgggatttc ttcctgtgta aatcgaaaaa atccgtcttc ccagtatatc
     9361 atgtcataaa tcgcgtcttc gccttgaacg tgaccgagcg tagcattaaa aatttgacct
     9421 ttcataaaat acacgttgcc ttcgatcgta ccgtctgaaa tcagcaggtt gcctgtttta
     9481 gcggtattgg ctaaaagctg gagaacgtcg atcacactga cgtcgccgag tttaccgaga
     9541 aatccgtcac gcaacgtcgc tttttctttt tcaaaagcat taacctgact gaccatgctt
     9601 tcgatccgaa gcaaaagatc ttgtcgtttg gtatggcgtt tgacgagata atcattggca
     9661 ccgcagcgga agccgcggat ttcggtcagc atgtcgtcaa tggccgtaaa aaacacgaat
     9721 ggtacagttg gcacgttgga tgtatcgcga atccgttggc acaactcaaa accattcatc
     9781 tcaccaagaa aaatatcgct gattaccaga tccggttttt cgcgattgac gatttccaag
     9841 gccacgtttc cgttgccggt atcaaaaacg atataatctt ctttgataag attttcttta
     9901 ataagcaagc gcgcctgttc atcggcatcc acgaggagca gtttggagcg agtttttttt
     9961 gtttctgcca tagccgtttt tctcctcagc ttcagttcag tttgttttca tcggtgatgc
    10021 ggcaatattc catcaaaata ttcatggtgg ccgaatggat ggtcggtgtc acctttggtt
    10081 caacggattc aaatttaaag accccagttt tccattcgcc gagattatac acagcttctt
    10141 caccgacaaa ctgtttgtat tcggcatgca acatgcgtcc gtcttcgaat gtgatttcgg
    10201 ctttgtcgct gtcacgggtg atcgtcagca agcccgtttt tttattcata atcaaaagtt
    10261 ggaatacatc cacaagactt aagtcgctaa atttgccata caagccttct tgcatctgat
    10321 tttcggcttg tttgtattcg tttgattttt ggagcatggc ggatactttt ccgaccaact
    10381 cttctttttt aatattcgat ttgatgagat agtcgtccgc tccggtacga aggccgcgta
    10441 attccgttgc aacctgatca atagacgtca gaaacataaa gggcaccatg ggtgattttg
    10501 aagtttctcg tactttgcgg caaaattcaa aaccgtccat ttgcggcatg ataatgtctg
    10561 atatcacgag atcgggtaaa tgatcattga ccatctgtaa accttcggcg ccattttcgg
    10621 cttcgatgac ttcaaaattt tctttgcgca aattaatgct taataatgtg cgcacggttt
    10681 tgttatcgtc aacgatcaga atttttttag gcatcggaaa aagtccttac tctctttatt
    10741 tcataaatta agcataaatt ccacggagtt ttaaaacctg agtgacgcgg tcaagcgcca
    10801 gtatatatgc gccgatgcgc atggattggg tgtggcgaag tgcgacggca tgcaccgatt
    10861 cgaaactttc gatcatcaca cgttcaaggc gatcatgtac ttcatgcaat ggccaaaaaa
    10921 aacccattcg gttttgcacc cattcaaaat aggaaactgt aacaccaccg gcattggcta
    10981 aaatgtccgg tacgaccaag atgccgcgtt catgcagcaa agtatcagcc tccggcgtga
    11041 tcgggccatt ggcaccttcg catattattt tagcctgtat tttggatgcg ttctccgcat
    11101 gaatttggtt ttccatagcc gcaggtatta acacatcgca atccaacatc aataattctt
    11161 cattcgttat tttctgtgca ttggggtatc cggataaaga acgacggttt tgttgagcaa
    11221 atcggatcgc atctgagata ttgatacctt tttcagaaaa gtaagacccg ctcgcatctc
    11281 cgatcccgat gattttgcat ccgttttcat acaaaagttg agcggcggtc gcaccaacgg
    11341 aaccaaaccc ttggattacg atgcgagctt cgttcgccgg tatgttacgc tgtttcatcg
    11401 cggcaagagc aatcagcata ataccgcggc cggtggcgtc gcgtcttccg tgagaaccgc
    11461 ccatgatgag cggttttccc gtcaccacgg cggtctcggt tcgccggaca tgcatgctgt
    11521 atgtatccat gatccatccc atttcgcgtt cgccggtatt gttgtccggt gccggtacat
    11581 ctttatcagg accgaaaatt tcaaacaaat tggccgtata acggcggata attttttcgt
    11641 acgccgattc cgtgagtcga gaaggatcgc actcaatagc gccttttgct ccgccgaatg
    11701 ggatatttac tacggcgcac ttccacgtca tccatgcggc caaagcggtt gtttcatcaa
    11761 gcgtggcttt cggagaaact cgaatccctc ctttggaggg gccccgaacg gtgctatgta
    11821 tgacccggaa gcctttaaaa acttggattg agccgtcttc aagtactact ggaatggata
    11881 cagtaacttg tttttcaggc gtaaggaggt attggcgaac acctgtatcc aattccaaaa
    11941 tatccgctgc cttttcaaaa ccacgcatca ttggggatga attctaacat ttaaaaagta
    12001 aaacaatacg tccgatggtg attactatca gcatgcatag caattgcaga tcgtaattga
    12061 aaatacagtc gcttaagttg aaaataatat taaagataga gcaaaaaatc aaatagattt
    12121 atatctagtt tcaggccaat acgtcatctt agaatagatg aagatggagt gccatattaa
    12181 gggtataacc ggataagtca attgtgcgtg aaggaatgtg tttttcgtgc gtttccataa
    12241 taaattcacg aggaccaatt tgtttgcgaa taacatattc aaaagattcc gaaggaagtt
    12301 ttattttctc ggtgattctc ttttcgtagc gaaattgaca gctgatttta ggagacaaat
    12361 aaaaatccaa tgacaaatca gctacggctc ccagcgcatt tcggtcgtat gaaaatgaag
    12421 atgaagtttg atttatgagt tgtggatggc tgtgatatcc aatattttgt gcgtaatagt
    12481 gatgcgatga ggtatgatat gaataatatg cgatacctgc accagtactc aattccagtc
    12541 gtgtagtcat attaacaggc ataaatatat atctaccgcc tacctgtaac attttaattt
    12601 ctgatttttg ttctaagtca aatctttggg aaggcggagt atcttcttcg ttaacaaatt
    12661 gtaaccaatt gcttcctgct aatgtttgat taaaaattct tgacacaaca gcctctattt
    12721 gccaatgtgg tgaaaggtta tgttgaacag atatttgaat taattttggg ttatgttttt
    12781 tttcatcagg atctttcaaa tcagccacat gatatacata accttcatta tcataaaact
    12841 gttcgatatt gtttcgttca tgaagataag aagcgccaaa aatgaaattg atatgccaat
    12901 aggatggctt ataatcagcc atatttgatt ttattttatt tcgttttgcg taatcggctg
    12961 ccgtaataga gtctaaagtg ttagccgaat gtttggtaac atcaggaaca taaggtcgtc
    13021 tattgatttc agggtcatat ctatcctgcg tatataatgg aaaaccagaa caatataaac
    13081 cgattgccat ccaaaacatc aatatcgtac gtaatggatt tgttgcacac actatacaac
    13141 acatatcaat ttctccccga aattactcag actacttaaa atgtttttat ttgttttttg
    13201 caggaaacat agtttgccat acgtaatccc aaatgggtgt actgactccg aacccttttt
    13261 ccgggttctg aaaatgatgt ttcatgtgat gattttttat agctaaccaa aatgccgaat
    13321 gcatgttgaa gtgatgaatg gcataatgcg tcatatcata ccacaaatac ccgatcagaa
    13381 aaccggcaaa catgggagcg atatttgatc caaatatgat taaaaataaa aaatagaata
    13441 acgcagctaa cgggatgctc gctgccgggg gcatgacaag gcgtttagaa tcattgggat
    13501 aatcgtggtg tacaccgtga aaaatgaaat gtatgcgttc catcagagcg ctccgcggtg
    13561 tgaaatgaaa aataaaacgg tgcaaaacat attccgtcag ggtccacact gcaagtccgg
    13621 ctaaaatcca aaacaaaaag tgttttatat ctgatttttc aatccataaa ctttgataga
    13681 tgaagtaaga aattatagga ataaatacga tcaacggaac ggagaagtgt actttggaaa
    13741 agaactccaa aaaatcactt tcaaacattc gcggcgtgac atctttgttg gtaatgggtt
    13801 tcttgctcat gtaagtattt cttaaatatt atatgaaaat aactcacatc gcgatgtgca
    13861 tcttttttgg gggatgttat acgatcaacg atatgagatt taatgatgcc tttcttaagg
    13921 taatagtagt gattttgtat agaaaatcaa gttgtttttt gaactgaaaa atgtacttta
    13981 ccttagctta aagataaaag tattatattg aactgaattt ttgatatagg atcaaattat
    14041 atgtttgaga cgcacatcat cgaatacagc gccaagtccg aaatgtatgc ggcattgcat
    14101 gcccagattc atcatttgct tgatggtgaa accgatctca ttgccaacgc agctaacgcc
    14161 tcatccttat tatttcatac gttgccggcg ctgaattggg cgggttttta tttttacaaa
    14221 ggcaatgaat tgatagtggg tccttttcaa ggtaagcctg cttgtgttca catcgctatg
    14281 ggcaagggag tatgtggtac ggccgctcaa cagagaaaaa ctgttgtagt gaaggatgtg
    14341 catactttcc ctgggcatat cgcgtgcgat agtgcgtcga attctgaaat tgtggtgccg
    14401 ctgatttcgg gaggacagtt attcggagtt ttggatttgg atagtcctca gatggctcgt
    14461 tttgacgaag cggatcaggc cggtttggag gaattcgttg atatttttat gaaacatacc
    14521 acttggccaa agaatttata gcttagtagc aagattgatt ttctttttta ggatacgtgc
    14581 tcttgcgcga agcgccgcac tgcctttcat ttccatttca tccaataatc cccaaactgt
    14641 aggtactaac cacgtatgcc gtaatgcgat atcagctaag ccggtcatcg tattgacggt
    14701 gacaaattta tttttagtgg tgtgtgcgag ttcaagcaaa cgcgtaccga ttcgtgtagc
    14761 atcgtttttt ttccacgcga cgtaacccaa aatcagaggc agatgcatct gtagggcttc
    14821 attgccaatc aactttggcc agtctttgtt cagatatggt gatagtgtac gtatggcggc
    14881 ggtttcgttt cgtaggattt tttctaacgt atcagaagcg cgaaaacaaa tatccgcatc
    14941 ttcgtgcgat aggagatggt ataaattttt aagccccgtt ctatcatgag taagcttact
    15001 cactagcaaa ttcgcatgag cgatggttcg gcgatcgttg ccggtaagca tttttattat
    15061 ttcactttta gtcatggctg tcgccaagcg ttatgtccaa gtcagctatg ggatgaccat
    15121 tgatgatcaa agtaattgca tgtttaccgg aataatgctt gcgcgtcgta aaattttgaa
    15181 aacgatgttt tttactcaac ttcaacgaac cgctatcgta aagaccttca cgccatttga
    15241 agactttgcg gctgattttt cctccggatt tgacaaaatc aatcgcgtat tcgatgcgaa
    15301 gtgttttggc tttccgtatc cgaatttccg tttcaaaatc caatgtatca ccgataaata
    15361 tttttttctt cttggatacg aatgaaagta ctgaaacgcc ggaggcatcg tgcgtaccaa
    15421 aaatagtgag cgctttttta ttgccccgtt ttaaaagtgt gcggcaggcg tgtttgacca
    15481 atttgtctgt ttccggagac aaacctcgcc agcgttctgc gatctgcaga acgatttccg
    15541 gattatcttt ggttatgtcg ttcaggttat tcgccacgct tcggcgaaca tattctgaag
    15601 gatcagcctt aagattttct aaaatcggca aaatcggagc cgggtccttt tgaaacacag
    15661 gcaatgcgat agcccacggc agtcgcggac ggcaaccctc gctggccaat cggcgtacat
    15721 gcgggctact gtgccgactc caaacgagca tttgttgtaa catgatgtcc ggataacgaa
    15781 gaataaaagg tctgacggcg aattctgcac ttgaaacttg ggtgaataat tccaatgccg
    15841 gtatggatga ctccaaatca tcaagtccgt agtgttctac gaaatccgga aacaccatat
    15901 ccgtaaacga tattttggtg tcattcatac gaaaatgttt cgttacatga atgagaatgc
    15961 cgagcgcctt tcggtaatcc tccggcaaat gagatctgag agcggaggaa atatgatgca
    16021 tccgatcttt gagttctcgc tgagaccatg cgtgatcaaa tataaaattc gtaaatgccc
    16081 gtttgtcaaa actcgaataa acggtttgaa tctgatcgcc taaagtttcg acaaacgatc
    16141 cggaaaacat attttttaat ggttctgcca taggttatgc cgttatttta aattccggat
    16201 tgtacatcaa tccgatcaca ttaccaaacg gatcacgcac tgatgcgatg ataagatcgc
    16261 cgccgacaca gtttggatgc tcgttggctt cagctccgga ccggataaga tgatcaaaca
    16321 tagcctgcac attgtcggtg cgccaatagg taatactgcc gaacccgggt gaattgtatt
    16381 gtttaggatc cggatcgagg ccaagttcga atccgccgaa ttgaaatccg acataaaaag
    16441 gttgatcgaa atagggcttt acgccaaacg catcagtata ccatcgtttg gctgcttcca
    16501 gatcggtgac gtgttgtact acagagcaca accccaatat tttggtattc atcgttatgc
    16561 tcctttcttt aagaaattat caagacgatt tttttgatac aagtgatgcc gaaaatgcat
    16621 ttggatcatg gcaaaccatt cgtcgccgct caaatatccg aatcgggggt gttgtgcctt
    16681 gccctgattt cgtttctgtg atagttcgct agctaccgtt cgcatggttc cgatcaacgt
    16741 gttcaaatcg cggcgcatgg cggcgatgga ttccggctgt ttgggggtgt acatgtcgga
    16801 aggggggact ttgatgcgta tcgggggaaa acggcccgta aaaaaaacaa tttttccggg
    16861 aaaagttttt ttctcatgag catggtccgg gttatccata cactgccgta ccatgcggag
    16921 atgaaaattt tgtgtgccgt gtacgaggtg gtaatacacc tgcccaatgg accaactttc
    16981 ttgatccggt gcgcgattga attttcgctc atcatacgta tccaaagcca tcatccaatc
    17041 gtttgcgatg gattcgaatt gatgtaagtg tgcagtgata ttcataggta atttcaaatg
    17101 aatgtttaat gtgaaaaacg tgaattaata gtggcgaaca tgaccgaaat agacatcttt
    17161 ttcgagagca ttcattaaac gagagcacga ttcggtggaa tgaaattttt caaaggcctg
    17221 atcggcatga tccttagtct tccatcgtaa aatatctaca aaaacacgcg acgtgtcgtc
    17281 ggcccgcata ttttcccatg atataaatcc ttcgtacgtg ccgataatgg atttggcttc
    17341 ggcgacaata tccggcatac gcgacacaaa ctccggtttg attttaataa cagccacttc
    17401 cgtgatataa tcgtttcctg gtgatgatat gttagcttga caggcattta aagtcaaaat
    17461 taacatgatg tttaaaaata ctgaaataca actttggaga ttcataggtg cctttcaatt
    17521 tagtttttgt taactatcgc acaaaaaata atgtgcatga ctgacatcct tatgtcagca
    17581 ggtttttaat ttttgataat tacccggctt gacaatagat gggacgtttt ctacattgcg
    17641 cgggtaataa accgaatcca ttattaaatg acattctcac aatccatgca ctccgacaaa
    17701 atgcgggtgg ctttgacctc cgtattggcg gcaatttttt taaccggtat gaaattggcc
    17761 gcaggtttag ctacaggcag tctcggcata ttatccgaag cggcccattc agcgctggat
    17821 ttgggcgctg cgcttctgac gtatgtagct gtacgtatat ccgataaacc ggccgatgac
    17881 gatcattcct atggtcacgg taaagtcgaa aacatttcgg cgctgttgca aacgggatta
    17941 ttgatcctga cttgcttttt tatcattcgc gcggccgtag agcgtttgat gtccggcgat
    18001 gtgcaggtgg aggtgaatgt atggagtttt gttgtaatca tagtttccat catcgtggat
    18061 tattcccgtt ctcgggcgct tgcgcgtatt gctaaaacct atcgcagtca ggcgctggaa
    18121 gccgacgcat tgcacttttc gtcagatata tggagttcgt cggttgtcat cataggcctt
    18181 atttccacag cgttgggatt tcatcaaggc gattcgcttg cggctatcgg cgtggcgtta
    18241 tttgtgcttg tgatcagcgg caaattactc aaagaaacga ttgatgcttt gacggattcc
    18301 atgcctaaag atattgaaca gcgcatcaga acggcgctgg tcagtttaga cggtgtggag
    18361 cggtatcgcc atttgcgggt tcgtcagtcg ggttcgaaag tgttcgtaga catgtatatt
    18421 gatgtcaagc gtacggtgcc tttcgaaatg gcgcatcatg tcgccgatgc tgtggaaacg
    18481 cgcattcatg aaattgtgcc caatgcggat gtgatcattc atatggaacc gcaggaaacc
    18541 gacgacgaaa gtatcattga taaaatccgg atgatcgtcg tacaagaagg tcttgcttgc
    18601 cataacgtgc gtgcacaacg ggttaccaac ggatattttg tggattttca tttggaatgt
    18661 gaaagccacc agcgatttga cgaagcgcat gaagcgtcgt cgcgcattga aagccgattg
    18721 atggaaaaaa tcccggagat taaggatgta aaagtgcata tcgaagatgc gcgggatcgg
    18781 gttgtgcaag catttgacat tacggaccaa agcaagccgc tcatcgccgc cgtacgggct
    18841 attgcagcta atacgcagcc ggtgcgtgca tgcagcgatt ttatcgtgat ggatatcgga
    18901 ggtcataaaa aattgatgct caactgcgtg gtgcccggcg atttatcgct cgatgaggta
    18961 cacgccataa cgacaaggct cgaaaaccga attcatcggg atataccgga ggtttcatcc
    19021 attgtcattc atccggagtt agcgcgtgcc tgaaccctcc atgattttcg aagagaaaga
    19081 cggattgcgt ctgtgccggt ttccggcgct cgaaaaattt cccgacgtga tggccgtgat
    19141 gtccacgcgc atcggcggtg tcagcgaggg ggattacaaa ggactcaatc tgggactgag
    19201 cacggatgat aatccgcacc atgtcgaaga aaatcggcaa cggttttggc gagcgctcgg
    19261 tgtagatatg aatgatgtaa ttcggcagca tcaggtgcac gaagatcgcg tcgttttttt
    19321 gcaggaacaa accctctgtg ccgataccga tgcgatgtat accgatcagt cgaaaaaatt
    19381 tctgaccgtc gtcgcggcag actgtgtacc ggttttgttt tatgagcccg cgcatcgtat
    19441 catcgctgtg atacatgcag gctggcgcgg aacgctgaaa caaattatca aaaaaacgat
    19501 ggcttctatt caacaaacat tcggatgttc attgggtcat gccgtggcgg tgcttgggcc
    19561 gtccatcggc gcggatcact acgaagtcag cgatgaggtg gcgtcaatgt ttgatacggc
    19621 gtttgtcaat cgtcgcgact acccgaaacc gcatcttgat ttatggaaag ccaatgccgc
    19681 acaattagat gaagccggcg tcggtcatat cgtcgtgacc ggtgcgtgta cgatgtgcca
    19741 accggatata tactattcgc accgcgcttc cgggggccgt tccggccgta tggtcggcgt
    19801 gattgggagg aggaactgac ccgatggatc atatcgaaaa atatttcgga tcattgatac
    19861 agcgtgccgg cgatgcgatt tttaccgtca gcccatcgca gattatttta tcgtggaacg
    19921 atggagcgcg tgaattgctc ggatatgaca aggaagacgt tcttggccac agtgtggatg
    19981 ttttatcgcc gctggatgac aagcgtcata tgcgtgcaat ggcaattgag gtgtttgatc
    20041 acggtgcgct caaaaatttg gaaaccgaac ttacccgcaa agacggacac gtcgtatcgg
    20101 cctacgttac cgtttcaccg gtgacggatg aaaacgaaaa tgtcgtagcg gtatcgttca
    20161 tcgccaaaga tataacggat cagaaccgac tactgattca cctcatcgaa agccaacgtc
    20221 aggccgggca gatacaagct ttagtcgaag cattgaccac gctgtcgcac catattcgca
    20281 atgcttccgc agtaatatcc gccaaaagtg aagtgtcgct ggaactcaat caactggccg
    20341 tttatcacga tctggtccgg gtatgtacgc atgaaacgcg tcgtataacg gcggtgattg
    20401 atactttgca ggacttggtg cgcgagatgc aagtcagcgg cgaaccgatt gatacggtgc
    20461 aaatgaacgg tgcgccggcc cctcttttag atatcgaagc ccgcctcaag gagcggctca
    20521 aaaagcttga tgaaaggccg aagtgacgcc gcaaaaggat tttgtttatc ccgatcttac
    20581 ctcgatagct gttattctcg tcgaaccgca agcacccgga aatatcggtt cggtcgctcg
    20641 cgccatg
//
LOCUS       DAOQGF010000057        20518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1215_length_20518_cov_7.779749, whole genome shotgun sequence.
ACCESSION   DAOQGF010000057 DAOQGF010000000
VERSION     DAOQGF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_20518_cov_7.779749"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PK937_09185"
     CDS             complement(<1..890)
                     /locus_tag="PK937_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH32785.1"
                     /translation="MKWFRWIGIIFLLGCSSIFAGNSNETENKTIGVISGRVTEENSG
                     APLAYANVAVAGTILGAVSDAEGFFKIIKVPQGIYTIVATYIGYEKKALDNVKVKTGE
                     TTHIEVRLAVSEITSEEIVVSATRKETTANLAPASVGVITAEEMQKKNISTFDQAIES
                     APSVQLTRSSGSNVQAVSIRGSSEVAGAGVGNRVLLLIDGRPALSPESGGALWNLVPT
                     NSIERIEVVKGAYSSLYGSSAMGGVINVITKNPSPVAVTRIHTEYGFYEKAPGWTGYD
                     TYNAFNTIEVAHSRRVGNVS"
     gene            complement(892..2154)
                     /locus_tag="PK937_09190"
     CDS             complement(892..2154)
                     /locus_tag="PK937_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH32786.1"
                     /translation="MKRHIRNKILRLSLIASTLFFLIKPVYAQIDASILRRHVQKLSG
                     DEMEGRGTGEPGEAEARGYIASEFKRYGLAAYGNDKGFLQKFEFRPRNGNNPHAAIDT
                     AQPARTAYNVVAYLDNGAENTIIIGAHYDHLGLGRQGGSLDPNPEGKIHNGADDNASG
                     TAGLLELARFYRQNKIKEKYNFLFIAFSGEELGLLGSKAFTANPSIDLNKIHLMINMD
                     MIGRLDSARRLMIYGTGTSPALENLVRNTSHDFQIKTDSSGIGPSDHTSFYLKNIPVL
                     HFFTGSHPDYHKPTDDADKVNYDGQKAVLEYIQRIIEKAEAMPKMAFTATRNNSTASD
                     SPRFKVTLGILPDYAFTGGMRVDGVTDGKPAAVAGLKAGDVIVQIGEHIVFDIQTYMQ
                     ALSKFTKGETTNVKIKRGEDTLDMKVVF"
     gene            complement(2157..3155)
                     /locus_tag="PK937_09195"
     CDS             complement(2157..3155)
                     /locus_tag="PK937_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32787.1"
                     /translation="MAQTPSSEKHLKNLRQLTFGGDNAEAYFSFDGKKLTFQSNNPAW
                     GLSCDQIFMMDIVSADSTYKPKQLSTGLGRTTCSYFLKGDTRIVYASTHLGGKDCPPP
                     PPSRSDRKYLWQIFDTYDIFVTDTEGRILQPLTQTPGYDAEATVSPQGDKIVFTSTRS
                     GDLELWTMNTDGSQLKQVTHDLGYDGGAFFSPDGKKLVFRASRPKTEDEIKEYKELLS
                     QGLVAPSNMEIYTCNVDGSDLRQITQLGKANWAPFFHPSGKKIIFSSNHHSQRGYDFQ
                     LYMINLDGNGLERITHESIFNAFPMFSPDGKRLIFSSNRNNNGTRDTNLFIADWED"
     gene            complement(3288..3866)
                     /locus_tag="PK937_09200"
     CDS             complement(3288..3866)
                     /locus_tag="PK937_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32788.1"
                     /translation="MKKTLLLSLILAIGIMILSGCDDDKKPTTATIKGTVTFKNVSVW
                     KASGSFTKADEDSGEVEVTIFADSVWKNGPGGRVPYGAPYNANNPVVFTRAAGDSTYQ
                     YEIEVEPGTYSALAVGFRTNRAVSSDKKTATLGVHWGMPDSVSHGIYIAPALPYFPGY
                     DYPAPAAITVEAGDELTLNFKADFGFVNSWFH"
     gene            4132..4707
                     /locus_tag="PK937_09205"
     CDS             4132..4707
                     /locus_tag="PK937_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNH32789.1"
                     /translation="MTDQEIIAIFQSAEGAKRDQVFGDIVHRYRERLYAIIMGFTNDH
                     DETDDLLQETFIKAYQHLHTFRAESSLYTWLCRIAINLSISYARKRKLRQFFSVDAME
                     EILPSGQDGPDATTEKMELRKHIKRAVDQLSEKQKKVFILRYYDELSHAEIAQITGNS
                     EGTIKANFFHAMNKVQAYLEKHYFEKGKPHA"
     gene            4700..5284
                     /locus_tag="PK937_09210"
     CDS             4700..5284
                     /locus_tag="PK937_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32790.1"
                     /translation="MRDEWVAYHKNKLSPEARRRFDENLISDPSENEAFQKFSRVAEA
                     LESARPDTPEGYWRNFNVHLMDRIAEKRKPRFSFSWKLAIPAIATAALAVFFLVPHNT
                     DDTTAVHPDVWNDLASSLESMETSSAFESGADIQIASEDVEETMVTADEWHDFSSALE
                     SIRFSVNETNADGTVWDKSAKEELLKTLDQTSII"
     gene            5307..5828
                     /locus_tag="PK937_09215"
     CDS             5307..5828
                     /locus_tag="PK937_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32791.1"
                     /translation="MKLFTILGVTLILGTSGVSLHAQEDAEFKERIIEIKKKKLLESL
                     ALSKDKENQFLKVYDEYFDKRRDLNRERKAVMKRIAHMSQLQADDIQGDKVTKAIEEL
                     GYVERKIVDQRQRTVEQLRGTLTEPELAKFVIFEQNFPIRMREMIFDIRARKHKDKDM
                     RMFIPPGEDDLLD"
     gene            complement(6004..7503)
                     /locus_tag="PK937_09220"
     CDS             complement(6004..7503)
                     /locus_tag="PK937_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNH32792.1"
                     /translation="MRRLFPPSLYNMVSMSGAALSVTSFAVFIFLFLIEITADHSNPY
                     MGIFTFMVAPAFLILGLLMIAYGAWREKKRIARGTPSEGHLPHIDLNDPRHRRATAVF
                     FFGTIILLGLSAFGSMEVLEYSDSVQFCGEVCHNVMHPEYTAYAHSPHARVPCAECHI
                     GSGAEWFVKAKISGAYQVYSVLFNKFSKPIPTPIENLRPAQQTCEQCHWPRHFFSEKQ
                     RALTYYVSDENNTKWTLNLLMRIGGGNELSGNTDGIHWHMNIANEVDYVSTDHKRQEI
                     PWVRIRDKKTGKETVYQSTENKISEAELKNHETRRMDCIDCHNRPSHKYNHPANQVNQ
                     YMQLGWIDPSLPYAKNISIKALEIPYTSTSRAMDSIAIVIRDFYSQNYPDISKHQSAK
                     VDKAIQETQKIYSRNYFPEMRADWRGYPDNIGHMYAAGCFRCHDGKHKSDDGKVISRD
                     CNVCHTLLAQKFENDKQEVSMQGLNYRHPVNVDKAWQEMSCYDCHNDKK"
     gene            complement(7516..9750)
                     /locus_tag="PK937_09225"
     CDS             complement(7516..9750)
                     /locus_tag="PK937_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNH32793.1"
                     /translation="MVFSRRILNTILIVIFLFAGFSLKAQSNDDCMECHSDESLSKKK
                     AGKKISLFVDEKIRGKSAHAKVNCIACHTGFDPEEIPHKEKITPVNCVSCHNKELTKH
                     AFHPNIMATKGTGWSDDANCKTCHGTHDVASTKVPGNIFHKDNLVNGCGSCHKDVKED
                     FIHSAHAEAQKRGDKNAPTCIQCHSSNITHIAAGMDSLQLKVAQAKVCMSCHVDNQEV
                     SSKTLLGAQFVRSFEKSVHGQALKNGKAEAANCVDCHGSHTMNKAIVDNSPVNKMHIP
                     ETCAKCHKDIANDYNSSIHGNMLHKGSKDAPGCTDCHGEHDILKHDDPKSPVSSRNVS
                     QQVCGTCHASVKLSKKYGIASDRFQTFSDSYHGLSVRGGALEVVNCASCHGAHNIKQA
                     KDSTSTISKANLVKTCGKCHPGAGDRFTIGSVHSSEEKKADDPLLWYVATIYLILIFV
                     VVGGMALHNIIDIHRKVRRKLAIQRGELHEEEHHGRGLYVRMTASERLQHGTMALSFI
                     ILVITGFMLRYPDAWWVVGIRSLSEYAFEARSILHRVAGIAMVVVSLYHLWYIIFTVR
                     GRQLVKDLWFCKKDWADAVGILKYNLGISKDKPRLGRFSYVEKSEYWALVWGTIVMAL
                     TGAILWFEVQSIDMITKLGWDVSRIIHFYEAILATLAIIVWHFYFVIFNPDVYPMSFA
                     WLTGKISEKEMEHEHPLELEAIKKSEEANRPQDEVVRVSQENVSDAKADDTPDENNIN
                     NSKN"
     gene            complement(9960..10328)
                     /locus_tag="PK937_09230"
     CDS             complement(9960..10328)
                     /locus_tag="PK937_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH32794.1"
                     /translation="MKKLMFIAAFGLLVMFVNVAQINAQEAKPADGKAIFEANKCMSC
                     HAVKNIGIEAKKAEGEEAEENKAPDLSGVGLEKDAEFIAKYLQKLEAINGKKHMKKFK
                     GTEDELKTLAAWLAEQKTKQ"
     gene            complement(10548..10916)
                     /locus_tag="PK937_09235"
     CDS             complement(10548..10916)
                     /locus_tag="PK937_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH32795.1"
                     /translation="MKSIVAFAIAFFATILMLSSKAYAQEAKPADGKAVFLSFKCESC
                     HAIKSMAIEAKKAEGEDAGDNKGSDLSGVGLKRDADFIVKYLQKLEAIEGKKHMKKFK
                     GTEEELKMLASWLAELKTKE"
     gene            complement(11001..11786)
                     /locus_tag="PK937_09240"
     CDS             complement(11001..11786)
                     /locus_tag="PK937_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH32796.1"
                     /translation="MMNVKVINLESLEGFGLHPDSGRNFLIHISSINLWKGLSIALVF
                     HAALITSYYVSAVLLKEDISMRTVRFVDANELTSAAPLEKTAEPVKVDVQMNEIPKVI
                     AGIPIVVPDAEAPSDVTLQTQEEIKTNIAASTFSLGNAGEGEIKVDISESLRGLNESS
                     DEPGMDEFIPVEQAPILLAKVIPEYPEIARQAGIEGKVFLKALVNEEGVVVKVVYIQG
                     DDMFKEAAVNALSQARFKPAINGNRPVKVWISYPFIFRLRTTQ"
     gene            complement(12175..12585)
                     /locus_tag="PK937_09245"
     CDS             complement(12175..12585)
                     /locus_tag="PK937_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32797.1"
                     /translation="MTRQLKLPLLILIFAFTACNSDQNDSGPDKRLANTQWRNRLLTF
                     PGNRHYYAFDEAGGGGQFAWPKDSCYVLDSLEVRDGKLISNDKEYAYRLSFGSTLLIV
                     TYPIYYAGDSEDVSYIDVAFERMAYSADTLDICD"
     gene            complement(12676..13947)
                     /locus_tag="PK937_09250"
     CDS             complement(12676..13947)
                     /locus_tag="PK937_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH32798.1"
                     /translation="MFQAKWLVTRSVGIKFKVLSRDGYYYSSLKLSQKMMLKHSYLIL
                     ICILCSCGGCLDEGGDQLLDLKEVWKVKVTQGLYSYTIPAATTSTVTFATTTGEVHGF
                     SIQSGAKKWATKVGQTSQPIVNRFRAYGGHIYFDYSNRLICFDGESGAIDWQFVKSQT
                     YGTLSDPEPSEEYVFTGDSYTPSGYNEGNIFCVLRGSGQLNWKRRLSEGVNNIAASTD
                     GYVYVTTQGFRMHPERGEVPYGLVYCFNSQTGDLIWSYDVSNRPNAGFVSGAADCRAA
                     IWQDRVFIVNSSGSVFCLNRFTGTEIWIKDLQSGSFAGMTVDDANQRGFVVAGIEFYC
                     LTLDSGNLVWRSNSRIGESAEWEPELFNGVVYNMGSVGFVEARDALNGQLLYQSIKNH
                     ETFFRFGSLQDDCFYMLNNNDNAIMKLKPLR"
     gene            complement(13904..14983)
                     /locus_tag="PK937_09255"
     CDS             complement(13904..14983)
                     /locus_tag="PK937_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32799.1"
                     /translation="MTLGTPHEGAFIAESTYNGNLSYYVAVQGNAILSPVAYVLGPDW
                     AEALWSIRDIVVGADLMNMFLASPAMNDLRPGSAGIQYLNSNNIETFNRVGIIGLDLD
                     PAHEPYRLAFTQLANDPQLGADIQDFLYAVYAIVAYIYWEYGDWLWDEGYISDAYTAW
                     AIGDIFYASAVDIAYAGYHWKIVNGSIIYSNGDYGWTYSDGVVPDYSQDYPNANRSPR
                     IIYNTNHLLEGRSNRGKDAISLGLNDVGFVHMTPPPAPITVSVTPQPSQAPAGGSYTF
                     TANVANGSGNYTVKWYSYYNEGGGTGGEIAMLKEGFVPPGGGTWTYIGTGPTKVFQPS
                     SKHQLRADATDNVSGQVASYTISWY"
     gene            complement(15020..15316)
                     /locus_tag="PK937_09260"
     CDS             complement(15020..15316)
                     /locus_tag="PK937_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH32800.1"
                     /translation="MKTINHIFICFLFATGLRAQENVVFLHGINSSGGMWTGSYNMIA
                     KLKQSFYMNATNPTRDDQTTFSVQADDVKTSLNPNINNYIAVGHSMGGLAARTY"
     gene            complement(15331..16173)
                     /locus_tag="PK937_09265"
     CDS             complement(15331..16173)
                     /locus_tag="PK937_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32801.1"
                     /translation="MKNNILIVASMFLMVSCDDASDKNGPIAPEINAPKKTTATTTIS
                     YTATTEVEEDDTFMPYAVPDVSGNTAAGLGPNHTVTYSQNVSLTQTDGITTSMTITFA
                     PLTGAADSLLALGRPSSIVYDGSTVRMYNRNGVLIPGNPYLPQSLSLNPAGDVQQQSV
                     SSKRCSKPANIFVEPKDLLTTAREQFDAVEKVAGNEWKFTIDRPFGKIELAYDDRLKT
                     ITSEKVYRDGELFLQKSYEFAESQGRKHRSKTKVTKTQKLPGGKQRTRTYTETVSNVS
                     ITNN"
     gene            complement(16982..20305)
                     /locus_tag="PK937_09270"
     CDS             complement(16982..20305)
                     /locus_tag="PK937_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH32802.1"
                     /translation="MIRRFFIGTVLFWLSALHAQQNITFETLSIEQGLSQTVVYSIVQ
                     DSRGFLWFGTQDGLNRYDGYNFTVYKNNHLDTASLSDNRILKVFKDRSGLLWIGTADG
                     GLCRFDRDKESFVIYRHDSKNSNSINSNRVNFIAEDASGNLWLATSGGLDVLDKTTGV
                     FTHQDKSKPIGKALPSRQVNAMLFDTKGRIWIGTNKGLISHELATGKTVKFEYGIGTG
                     KIIHENILSLCEDHGGMIWIGTAEGLNMYDPVTDRFVTFTNNPSDPASLSHNMVWSVF
                     EDHAGVIWVGTQSGLNRYDREEKRFMRFVNDPTDKSSISHSYVNTIFEDNAQRLWFGT
                     YGGGVSRFDPHQQKFSLYVSDAKNKTSLSNNVVWSFYEDVKKQIWVATEYGLNQLDPV
                     KNTFNHYFADASLKNNADNSYNCLLEDSKGNLWVGSYGSGLRRFDRASGRFVASYKHS
                     VDPASLIENRIITLLEDRDGHIWVGTMNGLDLFDPQKNGFIHYQNNPDDPASLRNNVA
                     KVLYQDRSGALWVGTQGGLSVRLPGETAFRHYLHDPRNLSTISHNNIRAITEDTSGTL
                     WIGTTGGLNRFDRIQSTFQHYTTKEGLPSDIIYGLLCDAKNRLWISGNKGLTRFIPSD
                     ASGSSGVFRVYDVSDGLQSNEFRNGSAYKAQNGEMYFGGIRGFNRFHPDSVSDNPNIP
                     NVVLTQFKIFEKPVALGKDINDLPVVELSYKQNFFSFEFAALNYTSSEKNQYAYRLEG
                     FDNGWVNIGTERKANYTNVDPGTYVFRVKGSNNDLVWNEKGVELKIVIQPPFWQTWWF
                     RIAALALLIFSAVTWYKQRMRRLLHQKQLLEKQVADRTSQLRQKTEQLETTLEDLQKT
                     QSQLVQAEKMSSLGQMVAGIAHEVNTPRATVDHYVFILYRKLQDAVQSQNPNDMRAAL
                     ENIQEEIVPPIELATERIGEIVEGLLNFARADHKDYRMSDVHKLINSALVIAKNQYKG
                     RIEIIKNYGEIPQIECFPGQLNQVFMNTIVNAVQATPGQGTITITTEMADEKNIRVRI
                     KDTGMGMSEEVKKKIFDPFFTTKPVGQGTGLGLALAYEIMGKHNGKIDVDTSPGKGTE
                     FIFTLPIEQPKKELGTVLS"
     gene            complement(20324..>20518)
                     /locus_tag="PK937_09275"
     CDS             complement(20324..>20518)
                     /locus_tag="PK937_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32803.1"
                     /translation="QKVKEEHGDKQYYKIIYSGYSEMEKDMIKMIASKVINEFLTKSC
                     PTKLLYKTVASGVEKSKTMI"
BASE COUNT         5567 a         4848 c         4311 g         5792 t
ORIGIN      
        1 tacgatacgt taccgacgcg gcgactatgc gccacttcga tcgtattaaa tgcattatac
       61 gtatcgtaac cggtccagcc gggagctttc tcataaaaac catattcggt atgaatccgt
      121 gtcacagcga ccggtgacgg attttttgta ataacattga tcacaccgcc cattgccgat
      181 gacccataca gcgaggaata agcgcctttg actacttcaa tgcgctcgat ggagttggtc
      241 ggaaccaaat tccacaacgc accgccggat tccgggctca gcgcaggtcg cccgtctata
      301 agcagcaaca cacgattgcc gacgcctgca cccgccactt ccgatgaacc gcgaatcgaa
      361 accgcctgca cattggatcc cgaagagcgg gtcaattgta cactgggcgc agactcaatc
      421 gcctgatcaa aggtcgaaat attttttttc tgcatctctt cggcggtgat cacaccgacg
      481 ctggccggcg ccaaattggc tgtggtctcc ttacgtgtcg ccgacacgac gatctcttca
      541 gatgtgattt ccgaaacggc caaacgtacc tcgatatgcg tcgtttcgcc ggtttttact
      601 ttcacattat ctaaggcttt tttttcgtac ccgatatacg ttgcgacgat cgtatatatc
      661 ccttgaggca ctttgattat tttaaagaac ccttccgcat cggaaacggc accgagtata
      721 gtaccggcga cggccacatt ggcatatgcc aacggtgcgc cggaattttc ttccgttact
      781 cgaccgctga tcacaccgat cgttttgttt tcggtctcgt tactattacc ggcaaatata
      841 ctagaacaac ccaaaagaaa gatgatacct atccatcgaa accatttcat atcaaaacac
      901 cactttcata tccaacgtgt cttcgccgcg tttgattttt acgttggttg tttcgccttt
      961 tgtaaatttt gacaacgcct gcatgtatgt ttgaatatca aaaacgatgt gctctccgat
     1021 ttgaacgatg acatcaccgg cttttagacc tgctacagcc gccggttttc cgtccgtgac
     1081 accatccacc cgcatgccgc cggtaaacgc gtaatccggt aaaataccaa gcgttacttt
     1141 aaaccggggc gaatcgcttg cggtagaatt attgcgcgtc gccgtaaagg ccattttggg
     1201 catggcttcg gctttttcga tgatgcgctg gatatattcg agtacggcct tttgaccgtc
     1261 ataattgact ttgtcggcat catccgtcgg cttgtgataa tcaggatgtg agcccgtgaa
     1321 aaaatgcagt acgggaatat ttttcaaata aaaactagtg tgatccgaag gtccgatgcc
     1381 gctggaatcc gttttgatct gaaaatcatg tgatgtatta cgaacaagat tttctaatgc
     1441 cgggcttgtt cccgtgccat atatcatgag acgtcgagcc gaatccaatc gtcctatcat
     1501 atccatattg atcatcaggt ggattttatt gaggtcaatg ctcggattgg ccgtaaacgc
     1561 ctttgatccc aataaaccaa gctcttcgcc ggagaaggca ataaataaaa aattatattt
     1621 ttctttaatt ttgttctgcc tgtaaaagcg agccaattca agcaaccctg ccgtccctga
     1681 tgcattgtca tcagcgccgt tgtgaatttt tccttccgga ttgggatcaa gagaccctcc
     1741 ttgccgacct aatccaaggt gatcataatg cgcgccgata atgattgtat tttcggcgcc
     1801 gttatccaaa taagcaacca cgttatacgc ggttcgggcc ggttgcgcag tatctattgc
     1861 cgcatgcggg ttgtttccgt tacgcggtcg aaattcaaat ttttgaagaa accctttatc
     1921 gttaccgtaa gcagctaagc cgtagcgttt aaattccgaa gctatataac cccgcgcttc
     1981 cgcttcaccc ggttcaccgg ttccgcgacc ttccatttca tcaccgctga gtttttggac
     2041 atgccgtctc agtatagatg cgtcgatttg tgcatatacc ggtttaatca aaaaaaacaa
     2101 tgttgaagcg atgagactga ggcgtaaaat tttgttacgg atgtggcgtt tcatgatcaa
     2161 tcctcccaat cggcgataaa taaattagta tctcgtgttc cgttattatt acgattggac
     2221 gaaaaaatca agcgtttccc gtccggcgaa aacatcggaa acgcgttaaa gatgctttcg
     2281 tgcgtaatgc gctccagtcc gttgccgtca agattgatca tataaagttg aaaatcatag
     2341 ccgcgttggg agtgatgatt ggatgaaaaa attatttttt ttccggacgg atgaaaaaac
     2401 ggcgcccagt tggccttacc aagttgggtg atctgccgca gatccgaacc atctacattg
     2461 catgtataga tttccatatt gctcggtgca accaaacctt ggctcaaaag ctctttgtac
     2521 tctttgattt catcttcagt tttgggacgt gaagcacgga aaactaattt tttcccgtcc
     2581 ggcgaaaaaa aagcgccgcc gtcgtaacca agatcgtggg tgacttgttt gagctgcgaa
     2641 ccgtcggtat tcatagtcca aagttcgagg tcaccggaac gcgtactagt aaaaacaatc
     2701 ttatcacctt gcggtgaaac cgttgcttcg gcatcataac ccggcgtttg cgtcaatggt
     2761 tgtaaaatac gcccctcggt atcggttaca aaaatgtcat acgtatcaaa aatctgccac
     2821 aaatatttgc gatcactacg ggagggcggc gggggcgggc aatcctttcc gccaaggtgc
     2881 gtcgaagcat agactatgcg cgtatcgcct ttaagaaaat aagaacacgt cgtgcgacct
     2941 aatcctgtgc tcaactgttt tggtttgtac gtagagtcgg cgcttacaat atccatcata
     3001 aaaatctgat cacaggataa gccccatgcc ggattattgc tttgaaaagt caacttcttt
     3061 ccatcaaaac taaaatacgc ttccgcattg tctccgccaa acgtcaattg acgcaagttt
     3121 ttcaaatgct tctccgatga cggcgtttgc gccatcacta cacccgtcac cgtcaaaaga
     3181 acccatcgca acagtgcact catttttttt catccctgtt tcatataaag aaaaaaggca
     3241 cgtgacgaca aaagagtcat cgtgcctttt tttaaaacca tattttatta atggaaccag
     3301 ctattcacaa aaccgaaatc agctttaaaa ttgagcgtta actcatctcc ggcttccaca
     3361 gtaattgccg ccggtgcagg gtagtcgtaa cccgggaagt aaggcagtgc cggagcaatg
     3421 tatataccat ggcttacgct atccggcatt ccccaatgca ctccgagtgt tgcggttttt
     3481 ttgtcggagg acactgcgcg gttagtacga aatcctacgg ccagcgccga gtatgtaccg
     3541 ggttcaactt caatttcgta ttgatatgtc gagtcaccgg ccgcacgggt aaagaccacc
     3601 ggattgttgg cattatatgg cgcaccataa ggcacgcgac cgcctgggcc gttcttccaa
     3661 acggaatcag caaaaattgt aacttctacc tcaccactgt cttcgtcagc tttggtaaaa
     3721 ctaccggatg ctttccatac gcttacattt ttgaaggtta cggttccttt gatggtagcc
     3781 gttgtcggct ttttgtcatc atcacagcca gataatatca tgattcctat ggcaaggatg
     3841 aggctaagta ataatgtttt tttcataaaa ttgccttgta taatggtagt aggtttaatg
     3901 acacttgcaa tatagagcca tagaatacca cttgtcaatg cggtgagcta ccttttacaa
     3961 atatattaca aacaatgaga tatgattaaa atttttaaac tatatgacta aaatcatggt
     4021 attttttaat cccttttgtt gtgatttttt gtatgaaatc tttacattac atttaaacct
     4081 tggtactgcg atcccgtcta atagaacacc aggagaagag aataccgacg catgacggat
     4141 caggaaatca tagcaatatt tcaatcagcc gaaggcgcga aacgtgatca ggttttcggc
     4201 gacatcgtgc atcggtatcg agagagactg tatgcgatca tcatgggttt taccaatgat
     4261 catgacgaaa cagatgactt gcttcaggaa acatttatca aggcgtatca gcacttgcat
     4321 acatttcgcg ccgaaagttc tttgtacaca tggctttgtc gcatcgcaat caatctttcg
     4381 atttcatatg cgcgcaaaag aaaattgcgt cagtttttca gtgtagatgc gatggaagaa
     4441 attttaccat ccggacaaga tgggccggac gcaacaacgg aaaaaatgga actgcgaaaa
     4501 catatcaaaa gagccgttga ccagttatcg gaaaaacaaa aaaaggtttt tattttgcgc
     4561 tattatgatg aactcagcca tgctgaaatt gcacagataa ccggaaattc cgaaggaaca
     4621 atcaaagcca atttttttca tgcgatgaat aaagtacagg cgtatttaga aaaacattat
     4681 tttgaaaaag gaaaacccca tgcgtgacga atgggtagcc taccacaaaa acaaattgtc
     4741 gccggaagcg cgacgccggt ttgacgaaaa cctaatttcc gatccctctg aaaacgaggc
     4801 ctttcagaaa ttcagtcgcg ttgcggaagc tttagaatcg gctcgtccgg acacgcctga
     4861 aggatattgg cgaaacttca acgtacacct gatggacagg atcgccgaaa agcgtaaacc
     4921 acgtttttct ttttcatgga aacttgccat tcctgcgata gcgacagccg ctttggctgt
     4981 ttttttcctc gttccgcata acacagacga cacaacggct gtgcatccgg acgtgtggaa
     5041 tgatttagcg tcttcactcg aatccatgga aacgtcctcc gcgttcgagt cgggcgccga
     5101 tatacagatc gcatccgaag atgtcgaaga aacaatggtg acagccgacg aatggcatga
     5161 tttttcaagt gctctggaat ctattcgttt ttcagtcaat gaaaccaacg ccgacggcac
     5221 ggtttgggat aaaagcgcca aagaagaatt actcaaaaca ctcgatcaaa catctataat
     5281 ctaataattc aatcataggt atgcgcatga aactttttac gatccttggt gtaactctga
     5341 ttctcgggac ttcaggtgta tctctgcacg cgcaggaaga cgctgagttc aaagaacgga
     5401 tcatcgaaat caaaaagaag aaactgctcg aatcccttgc actttctaaa gacaaggaaa
     5461 accagtttct caaagtgtat gacgaatatt tcgataagcg ccgcgacctc aatcgcgaac
     5521 gtaaggccgt tatgaaacgt atcgctcata tgtcacagct tcaggcggat gacattcagg
     5581 gcgataaagt gaccaaagct atcgaagagc tcggttatgt cgaacgtaaa atcgtagatc
     5641 aacgtcaacg caccgtcgaa cagctgcgcg gcactctgac tgaacccgaa ttagcaaaat
     5701 ttgtgatttt cgaacagaat ttcccaatcc gcatgcggga aatgatcttt gatatccgcg
     5761 cccggaaaca taaagacaag gacatgcgca tgtttatacc gccgggcgag gacgatttat
     5821 tggattaaat gaggtaggtc acaacttata ttttggcagg ataatatcga gacacaaaaa
     5881 ttaccttcgc acacttaact gaaggaaatc gaagcgtaga tgtaaacaca acatcaatct
     5941 acaattcacc gttttacacc aaaagcaaaa tatcgggata agtataaaac tgtaatctaa
     6001 aacttatttc ttgtcgttat gacaatcgta acaactcatt tcctgccacg ctttatccac
     6061 attaaccgga tggcggtaat tcaaaccctg cattgaaacc tcttgtttgt cattttcaaa
     6121 cttctgcgca agcaacgtgt ggcaaacatt acaatcccgt gaaatcactt taccatcatc
     6181 gcttttgtgt ttgccgtcat gacaacggaa acatcctgcg gcatacatat gtcctatatt
     6241 atccggataa ccacgccagt cagcacgcat ttcaggaaaa taattacgac tgtatatttt
     6301 ttgcgtttcc tgtatcgctt tatccacctt agcggattga tgtttagaaa tatcaggata
     6361 attttgactg taaaaatccc gtatcacaat cgcgatgctg tccatcgctc gggatgtaga
     6421 tgtataaggt atctccaacg ctttgataga aatgtttttg gcatacggca atgacggatc
     6481 aatccaaccg agctgcatat attgattgac ctgattagcc ggatggttgt atttatggct
     6541 tggacgattg tggcaatcta tacaatccat gcggcgcgtt tcatggtttt tcaactcagc
     6601 ttctgaaatt ttgttctccg tggattgata tacagtttct ttccccgttt ttttatcgcg
     6661 aatacggacc catggaattt cctgacgttt gtgatcggta gagacatagt ccacttcatt
     6721 ggcaatattc atgtgccagt gaatgccgtc ggtgttaccg gacagttcat tgccaccgcc
     6781 gatgcgcata agtaaattca gcgtccattt ggtattgttc tcatctgaaa cgtaatacgt
     6841 aagggcgcgt tgcttttcac tgaaaaaatg gcggggccag tggcactgtt cgcatgtttg
     6901 ttgtgcggga cgcagattct caatgggcgt cggtatcggt ttagaaaatt tattaaaaag
     6961 caccgaatac acctgatatg caccggaaat ttttgcttta acaaaccatt ccgcaccgga
     7021 gcctatgtgg cactcggcac atgggacgcg tgcatgcggt gaatgcgcat aggccgtata
     7081 ttcggggtgc atgacattgt ggcaaacttc accgcaaaac tgtaccgaat ccgaatattc
     7141 taaaacttcc atactgccaa acgccgataa accgagcaaa ataatcgttc cgaaaaaaaa
     7201 cacggcggtt gcacggcgat gacgcggatc attgaggtct atgtgtggta gatgtccttc
     7261 ggagggtgtt ccgcgcgcaa tacgtttctt ttcgcgccat gcgccatatg caatcatcaa
     7321 taaaccgagt atcagaaacg ccggcgctac catgaaagta aaaataccca tgtacggatt
     7381 gctatgatcc gccgtaattt cgataagaaa aaggaaaata aagacggcaa aactggtaac
     7441 cgacaatgcg gcgccactca tacttaccat attatagagt gacggaggaa ataatcgtct
     7501 catagttcgg ctccgtcagt ttttactgtt attaatatta ttttcgtcgg gcgtatcatc
     7561 ggctttagca tccgatacgt tttcctgact tacacgaaca acctcatctt gagggcggtt
     7621 agcttcttct gattttttga tagcttcaag ttccaacgga tgttcatgtt ccatttcttt
     7681 ttcagagatt ttacccgtca accaagcaaa actcatcgga tatacgtcag gattgaagat
     7741 gacaaagtaa aaatgccata cgatgatcgc cagtgtagcc aaaatcgctt cataaaagtg
     7801 aatgatacgt gacacatccc agcccaactt ggtgatcatg tcaatggact gcacttcaaa
     7861 ccaaagaatt gcaccggtca aggccatcac gatcgtcccc cacaccaaag cccaatattc
     7921 gctcttttcg acataactga agcgacccaa gcgcggtttg tctttactta taccaaggtt
     7981 atatttgaga ataccgacag catcagccca atctttttta cagaaccaca gatctttgac
     8041 caactggcgt ccgcgcaccg taaaaatgat ataccagaga tggtacaaac ttacgacgac
     8101 catcgcgata cctgcaacac ggtgcaatat actgcgtgcc tcaaaggcat attcgctaag
     8161 actacggatg ccaactaccc accatgcatc gggataccgc agcataaagc cggtaatcac
     8221 aagtattatg aaactcaaag ccatcgtgcc gtgctgaagt cgctcagatg ccgtcatgcg
     8281 aacatacaga ccccgaccat gatgttcttc ttcatgcagt tctccgcgtt gaatggccag
     8341 cttacggcgc actttacgat ggatatctat gatattgtgc agagccatgc ctccgacaac
     8401 gacgaaaatc aaaatcagat aaatcgtagc cacataccaa agtaacggat catccgcttt
     8461 cttttcttca gatgaatgca ccgacccgat tgtaaaacga tcgccggcac cagggtgaca
     8521 cttgccgcaa gttttgacta aattggcttt tgaaatcgtc gaggtcgaat ctttagcttg
     8581 tttgatatta tgtgcaccgt gacaacttgc acagttgacg acttccaatg ccccgccgcg
     8641 taccgataga ccgtgataac tatcggagaa tgtttgaaaa cgatccgaag cgataccata
     8701 ttttttcgat agctttaccg atgcatgaca ggtaccgcac acttgctgtg aaacattgcg
     8761 agacgatacc ggcgacttag gatcatcatg ttttaaaata tcgtgttcac cgtgacagtc
     8821 cgtacaaccc ggcgcatcct tactgccttt gtgcaacata tttccatgaa tactgctatt
     8881 atagtcattg gcaatatctt tatgacattt cgcgcatgtt tcgggaatat gcattttgtt
     8941 gacgggcgag ttatctacga tggccttatt catcgtgtga ctgccgtggc aatctacaca
     9001 gttggccgcc tccgcttttc cattttttaa agcctgaccg tgtacgcttt tttcaaaact
     9061 gcgaacaaac tgcgcaccga gcaatgtttt cgatgaaact tcctgattat ctacgtgaca
     9121 actcatacat actttggctt gcgccacttt gagttgtaac gaatccatgc ccgctgcgat
     9181 gtgcgtaata ttgctggagt gacattggat acaagtcggc gcatttttat cgccacgctt
     9241 ttgcgcttcg gcatgagcgg aatgaataaa atcttctttt acatccttgt ggcacgaacc
     9301 gcaaccattg accagattat ctttatgaaa aatatttccc ggtacttttg tcgaagccac
     9361 gtcgtgtgtg ccgtgacatg ttttacagtt ggcatcatcg ctccaccctg tacctttggt
     9421 tgccatgata ttggggtgaa atgcatgttt ggttaattct ttattatggc aggaaacaca
     9481 attcaccggc gtgatttttt ctttgtgcgg aatttcttcc ggatcaaatc cggtatggca
     9541 cgcgatacag ttgactttag cgtgtgccga tttacctcgg attttttcat ccacaaaaag
     9601 cgaaatcttt ttacccgcct tttttttgct gagcgactcg tcggagtggc attccataca
     9661 atcatcgttg gactgagcct tgagcgaaaa gccagcgaat aaaaaaatca cgataagaat
     9721 ggtgtttaaa atgcggcgtg agaaaaccat aacatgcctg ctgtctttat atttcagaat
     9781 ggatttttat gattaccaaa ctaccgtttt ttataacggc agctgttttt ccagtacagg
     9841 ctgttttccg atttgtaaag ttcaatacgt agtaaacggc ctgtttttta ttaaaaaaag
     9901 aatctggcca aaacgggttc ggccagattc ttatatcgca catatatgaa ctgtacgggt
     9961 tattgtttcg ttttctgttc ggccaaccaa gcggctaatg tcttcagttc gtcttccgta
    10021 cctttgaact ttttcatgtg ttttttgccg ttgatggctt caagtttttg aagatacttg
    10081 gcaataaact cagcatcttt ttcaagaccg acgccggaaa gatcaggagc tttgttttcc
    10141 tccgcttctt caccttcggc ttttttagct tcgataccta tgtttttgac cgcatggcac
    10201 gacatacatt tattggcttc aaaaatagcc tttccgtccg caggtttggc ctcttgtgca
    10261 ttgatttgcg ctacatttac aaacattact aataaaccaa aagcggcgat aaacatgagt
    10321 tttttcatag tattgcaaac tcctcttaaa tgtttttcta tcaattaaat atacattagt
    10381 ttcattgttc acagtcatac tatgtaaaaa ttatgtaaag aaacatgatt ttcgtcatct
    10441 tgataatttt tggtatatct cgtaagtgct taaaaataaa tcggctacag cgaatctgta
    10501 gccggatagg atggctaaat gtatgcaaat aatgaaacag taaccgatta ttctttcgtt
    10561 tttaactcag caagccacga cgccaacatt ttaagttcct cttcagtacc tttaaatttt
    10621 ttcatgtgtt ttttaccttc gatcgcttcg agcttctgaa ggtattttac tataaaatca
    10681 gcatctcgtt ttaaccctac accggataga tcggaacctt tgttgtctcc ggcgtcttcg
    10741 ccttcggctt ttttagcttc gatggccatg gatttgatag catgacatga ttcacattta
    10801 aaggacagaa ataccgcttt accgtccgcc ggtttagctt cttgcgcata agccttggaa
    10861 gacaacataa gaatcgttgc aaaaaaagcg atcgcgaaag caactatact tttcataaca
    10921 ctccttgata agtattgatt aatccgccac acatgtccta tgcacgcgac gcccgtgcgg
    10981 cgtagtgaga ataattcatt ttattgtgtc gtgcgaagtc tgaaaataaa tggatacgaa
    11041 atccacactt tgaccgggcg attaccattg attgccggtt tgaaacgcgc ctgcgacaaa
    11101 gcattcaccg cggcttcttt aaacatatca tcgccctgaa tgtaaacaac tttgacaacg
    11161 actccttctt cgttaaccaa tgctttcaaa aaaactttac cttcaatacc ggcctgacga
    11221 gcaatttcag gatattcggg tattacttta gccaataata tcggcgcttg ttcgaccggg
    11281 ataaattcgt ccattccggg ttcatcggaa ctttcattga gaccgcgaag tgattcacta
    11341 atatctactt ttatttcgcc ttcgccggca ttacccagcg aaaaggtcga agcggcgata
    11401 ttggtcttaa tttcttcctg agtttgtagt gttacatccg atggagcttc cgcatctggc
    11461 acgacgatgg gaattcccgc tattaccttt ggtatttcgt tcatttgaac atccaccttg
    11521 actggttcgg ccgttttttc aagcggtgcc gcgcttgtaa gctcattggc atccacaaaa
    11581 cgaaccgtac gcatggatat atcttctttg agaagaacag cgcttacata gtatgaagta
    11641 ataagtgccg cgtggaacac aagcgctatt gaaagaccct tccacagatt gatgctgctg
    11701 atatgtatta gaaaattacg gcccgagtcc gggtgaagtc caaatccttc aagactttcc
    11761 aaattgatga cttttacatt catcatagag ccgatcacct cctgctacaa ggtaaaacgc
    11821 aaaaggcata gagaaaatgc ccgacgtcat cagctgtttt gacttatgtc aaagaatagg
    11881 ccttaataat aaagatgtta caaaattacc gcataacgct acggcagtat caacctctca
    11941 aggttctccc ccacacgacc cattggggtt cgaacgacta cccacgccgg aggcatatcc
    12001 aaagcccgtt taatcacgat caccagacaa ggcacatttc gcttccagtt ccgacactaa
    12061 tgaaaacctc ggagcctagg ggcaccacgt tttgcccgca acagatagtg agcttagtcg
    12121 agctaagggc caaagaggta tcggttaaat ccctcaagaa cggactggaa agagtcagtc
    12181 gcatatgtcc aacgtgtccg cagaataagc cattctttca aacgccacat caatatagct
    12241 aacgtcttcc gaatctccgg catagtatat gggataggtg acgatcagga gtgtcgaccc
    12301 gaagctgaga cgataagcat attccttgtc gttggatata agtttgccgt cccgcacttc
    12361 cagagaatcc aagacatagc aggaatcttt tggccatgca aattgtccgc cgccacccgc
    12421 ctcatcgaaa gcgtagtagt gtctgtttcc ggggaaagtc aaaagcctgt ttctccactg
    12481 cgtgtttgca agcctcttat ccggcccgga atcattctga tctgaattgc atgcagtgaa
    12541 tgcaaaaatc agaataagca atggcagttt caattgcctt gtcatgatgt gtcctgtaat
    12601 aatcttcaaa gtttttccaa catgccacct ggctttagcc aaactaaaac aaggaagcga
    12661 ttcatttgag tacactcatc tcagtggttt aagtttcatt atggcattat cattgttgtt
    12721 caacatatag aaacaatcat cttgcagaga tccaaatcga aaaaaagtct cgtgattctt
    12781 tatgctctgg tagagtaact gtccattcaa tgcgtctcgg gcttcaacaa agccaacgga
    12841 tcccatgttg taaacgactc cattgaacaa ttctggctcc cattcagcag attccccaat
    12901 tcgagagttg gatcgccata cgagatttcc agagtccaaa gtaagacaat aaaactcaat
    12961 tcctgcgacg acgaatccgc gttgattcgc atcgtcaacc gtcatgccag caaaagaacc
    13021 gctttggagg tctttaatcc aaatttcagt tcccgtgaaa cggtttagac agaacaccga
    13081 tccgctggaa ttaacgatga aaacacgatc ctgccagatt gccgctcggc aatcggctgc
    13141 accgctcacg aatccggcgt tcgggcgatt ggaaacatcg taactccaga tcaaatcgcc
    13201 ggtttgcgag ttaaagcaat agaccaagcc gtatggcacc tccccgcgct cgggatgcat
    13261 acgaaatccc tgagtggtaa cataaacata tccgtccgtt gaagcggcaa tattgttcac
    13321 accttcggag agtctcctct tccagttcaa ttggccagaa cccctgagca cacaaaagat
    13381 attaccttca ttatatccag acggtgtgta cgaatctccg gtaaatacat actcctccga
    13441 aggttcagga tctgagaggg ttccgtaagt ttgtgatttt acgaattgcc agtcgatcgc
    13501 tccactttcc ccgtcaaaac agataagccg attggagtaa tcaaagtaaa tgtgacctcc
    13561 gtacgccctg aaacgattaa cgatgggctg tgaagtctga ccaacttttg tggcccattt
    13621 ttttgcgccg ctctgaatgg aaaaaccgtg aacttctcct gttgtcgtcg cgaaggtgac
    13681 tgttgaagtg gttgctgcgg gaatagtgta tgagtacaaa ccctgagtta ctttgacttt
    13741 ccaaacctct ttcaggtcaa ggagttgatc acccccttca tccaggcaac cgccacaaga
    13801 acacaaaata caaatcaaaa tcaaatatga atgtttaagc atcatttttt gtgataattt
    13861 aagggatgag taataatacc catcccttga taataccttg aacttaatac caactgatcg
    13921 tgtaactagc cacttggcct gaaacattat cggttgcgtc tgccctcaat tgatgttttg
    13981 agctcggttg gaaaaccttt gtaggcccgg ttccgatata tgtccaggtg ccaccgccgg
    14041 gtggaacaaa cccttcttta agcatcgcaa tctctccgcc tgttccacca ccttcattgt
    14101 agtaggaata ccatttaact gtatagttgc ccgatccatt ggccacattt gctgtaaatg
    14161 tataactccc gccggccggc gcttgtgatg gttgcggtgt gacagaaact gtgatcggcg
    14221 caggtggagg cgtcatgtga acgaatccga cgtcattcaa gcccagacta atcgcatctt
    14281 ttccgcgatt gcttctcccc tccaacagat ggtttgtgtt gtagatgatt cttggcgatc
    14341 ggtttgcatt tgggtagtcc tgcgaatagt caggaacgac tccgtcgctg tatgtccaac
    14401 cataatcgcc attcgaataa ataatactgc cgttgactat tttccagtga tagcccgcgt
    14461 aggcaatatc caccgcggag gcatagaata tgtcgccaat ggcccaggcc gtataagcat
    14521 cggaaatgta gccctcatcc caaagccaat ctccgtattc ccagtaaata taagcaacaa
    14581 tcgcgtacac agcgtataaa aaatcttgaa tatcggctcc cagttgcgga tcattggcta
    14641 gttgcgtaaa ggcaagacga taaggttcgt gcgccggatc gagatcaaga ccgatgattc
    14701 cgacgcggtt gaatgtctcg atattatttg aattcaagta ttggataccg gcgctcccgg
    14761 gccttaagtc attcatagca ggactcgcta gaaacatatt catcagatcg gcaccgacta
    14821 cgatatccct aatgctccac aaagcttctg cccagtcagg gcctaggacg taagccaccg
    14881 gactcaagat ggcattccct tgaactgcaa cgtagtacga taagttgccg ttgtacgtgc
    14941 tttccgctat gaaagcgcct tcgtgaggtg ttcctaatgt cacaatacga ttgatctttt
    15001 ttgcagactg ttcttttttc tagtaggtgc gtgcggcaag accacccatg ctatgaccca
    15061 cagcgatata gttatttatg ttcgggttca ggctggtctt tacgtcatcg gcttgaacac
    15121 taaatgttgt ttgatcatcg cgtgtcggat tggtggcatt catgtagaac gactgtttga
    15181 gttttgcaat catgttataa ctaccagtcc acatgccgcc actggaattg atgccatgca
    15241 agaacacaac attttcctgc gcgcgaaggc ccgtagcgaa aagaaaacat ataaatatat
    15301 ggttaatcgt tttcatttta gcctcgttgt ttagttgttg gtgatgctaa cattggatac
    15361 agtttcggtg taagtccgcg tccgctgttt accgcccggc aatttttggg tcttagtaac
    15421 cttggtctta gatctatgct tacgcccctg gctctcagcg aattcgtagc tcttctgtag
    15481 aaacagctcc ccatccctgt aaaccttttc actggtgatg gtcttcaatc tatcatcgta
    15541 tgccagttcg atcttaccga aaggccgatc aatcgtaaac ttccattcat ttcccgcaac
    15601 tttttctacg gcatcaaact gctctcgggc cgtcgtaaga agatcttttg gttcaacaaa
    15661 gatatttgcc ggcttcgaac aacgcttcga agaaacggat tgttgttgta cgtcacccgc
    15721 cggattcagt gacagcgact gcggcaaata tgggtttcca ggaatcagaa ctccatttcg
    15781 gttatacatt ctcacggttg atccgtcgta cacgatgctt gacgggcgtc ctagagccag
    15841 aagagaatcc gccgcacccg tgagcggtgc aaaggtaata gtcatcgacg tcgtaattcc
    15901 gtcggtctgg gtcaaggaaa cgttttgcga ataggttacg gtatggtttg gccccaaacc
    15961 cgcggctgta tttccgctga cgtcgggaac cgcgtaaggc ataaaggtat catcttcttc
    16021 gacctctgtt gtcgcggtat agctgatcgt tgttgttgcg gtcgtttttt ttggcgcatt
    16081 gatttcgggc gctatcggac cattcttatc gctcgcgtcg tcgcacgaga ccatcaaaaa
    16141 cattgaagct acgattagga tattgttctt catttctttc tcccattttt tcgttttatc
    16201 aaatgaattc cccgatactt gcgacggaga ggttcatttt ctgcaatcgt ggattatagc
    16261 atcggggcag ctatacatcg gtcatcactt cgtaagtgag gatgattgat aggtcatgcc
    16321 tgcttccaaa cgtacattcc atttccattc aaaatacttt acaatcacaa tactttggcg
    16381 gttccatgct ctcacctcca attcgtaccg gtgccgtacc ttgagattcg gtccgattct
    16441 caaaatcgtt cgtaattaaa gtagataata aaattgggga atccctagtg aactttaggg
    16501 agagagaaac tatttgtcaa gagtttttcg attttttgaa gattttgaaa tgcagattac
    16561 gcgcggctcc ggatgaactg tgaatttaat cctccgaccc cctccctcca actacccaat
    16621 tctattagta tttgaacgag tgtgagtgag gttgtgtaag aaacattctt tagtcgcacc
    16681 ttctgtgtga aactcgtgtg gtcaccaaag cgggagaaac aaaacgccgt tgcgaatttc
    16741 atatccagat gattgatgtt ttcatcgtgc tcaaagacat aatcttgggc ttcaagaatc
    16801 tgctccgagt cggacgatac agatcccatg gaaaactccc gccggcacac aaataacttc
    16861 aatcggaaat tcgacttgaa tcagaagcag accttataag gaattgaaag aatgatctca
    16921 tcttggagga tgctgattcg gattgcggtc ttcaagatca ttcccgatag tcccgaccga
    16981 gtcaggataa aacggtaccg agttcttttt taggttgttc gataggaagt gtgaaaataa
    17041 attcggtacc tttaccggga ctggtgtcca cgtcgatttt tccgttgtgt ttacccataa
    17101 tctcgtaggc taacgcaaga cccaaccctg taccttgacc gacgggtttc gtcgtaaaaa
    17161 acggatcaaa tatttttttc ttcacttctt cgctcattcc catgcccgta tctttgatgc
    17221 gcacacgaat atttttttca tccgccattt ctgtcgttat cgtgatcgta ccctgacccg
    17281 gtgtcgcttg tacggcattg acgatcgtat tcataaaaac ctgattgagt tgaccgggaa
    17341 aacactcgat ctgaggtatt tcgccatagt tttttatgat ttcaatccgg cctttgtatt
    17401 ggtttttagc gataactaaa gcactattga tcagtttgtg cacatcggac atacggtaat
    17461 ctttgtgatc ggctcgggca aaattgagaa gaccttctac gatttcaccg atacgctcgg
    17521 tagccagttc gatcggcggc acaatctctt cttgtatatt ttcaagagcc gcacgcatat
    17581 cattcggatt ttgagattga accgcatctt gcaatttgcg atacaaaata aacacataat
    17641 gatccaccgt tgcccgcggc gtattgactt catgtgcaat accggccacc atctgaccta
    17701 gggatgacat tttttcggct tgaacaagtt gggactgcgt tttttgcaga tcttccagcg
    17761 ttgtttcaag ctgttcggtt ttttggcgaa gttgcgatgt gcggtcagct acttgttttt
    17821 ctaaaagctg cttctgatgc agcaacctcc gcatacgctg tttgtaccac gtcaccgctg
    17881 aaaaaatcaa cagcgctaag gccgcgatac gaaaccacca cgtctgccag aacggaggct
    17941 gaataacaat tttaagttca acgccttttt cattccacac cagatcgttg ttcgaccctt
    18001 tgacgcgaaa aacatacgta ccgggatcga cattggtata attagctttg cgctcggtac
    18061 cgatattaac ccatccgtta tcaaatcctt cgagacggta cgcatattga tttttttcag
    18121 aactggtata gttgagtgcg gcaaactcaa atgagaaaaa attctgttta tacgaaagct
    18181 caacgaccgg aagatcattg atatccttgc ccaacgctac cggtttttcg aaaattttga
    18241 actgcgtcag aaccacgtta ggaatattgg gattatcgct tacgctgtcg ggatgaaacc
    18301 ggttaaaacc ccggatacct ccaaaataca tttcaccgtt ttgcgcttta tacgccgaac
    18361 cgtttctaaa ttcgttactt tgtaacccgt cactgacatc atacacacga aacaccccgg
    18421 atgaacccga tgcatcagac ggtataaagc gtgtaagccc tttattcccg cttatccaaa
    18481 gacgattttt agcatcacaa agcaatccgt agataatatc gctgggtaac ccttcttttg
    18541 tagtatagtg ctgaaacgta gattgtatgc gatcaaaacg attgagtccg ccggtcgttc
    18601 ctatccatag cgtacctgaa gtgtcttccg taatggcgcg gatattatta tgactgatag
    18661 tagacagatt gcgcggatca tgcaaatagt gacgaaacgc cgtttcaccc ggcaaacgca
    18721 cactcaaacc gccttgggta cccacccaca atgcaccgct ccgatcctga taaagcactt
    18781 tggccacatt attacgtaaa gaagccggat catcaggatt gttttgataa tgaataaaac
    18841 cgtttttttg cggatcaaac agatcgagac cattcatcgt acctacccat atatgcccgt
    18901 cgcgatcttc gagtaatgta atgattcgat tttcgatcag cgacgcgggg tctacggaat
    18961 gtttataaga cgcgacaaac cggccgcttg cgcgatcaaa acgacgcaaa ccgctgccgt
    19021 aacttccaac ccataaatta cctttactgt cctccagaag acagttatag ctgttatccg
    19081 cattattttt gagtgacgca tccgcaaaat agtgattaaa cgtatttttt acgggatcta
    19141 attgattgag tccgtattcg gtagcaaccc atatttgttt tttgacatcc tcataaaacg
    19201 accaaactac attattgctt aaggatgttt tattcttagc atcgctcacg tagagggaaa
    19261 atttttgctg atgcggatca aaccgggaca cacctccgcc ataggtgcca aaccaaagac
    19321 gctgggcatt atcttcaaaa atcgtattga catagctgtg actaatactg cttttgtcgg
    19381 tcggatcatt gacaaaacgc ataaatcgtt tttcttcgcg gtcgtagcga ttaagcccgc
    19441 tttgggtacc tacccagatc acacccgcat gatcctcaaa gaccgaccat accatgttat
    19501 gggaaagact tgctggatcg gaaggattat tcgtaaacgt cacaaaacga tcggtaaccg
    19561 ggtcatacat attaagccct tcagccgtac caatccaaat cattccacca tggtcctcgc
    19621 aaaggcttaa tatgttttcg tgaatgattt ttcctgttcc gatcccgtat tcaaatttta
    19681 ctgtttttcc tgtcgccaat tcgtgcgata tcaaaccctt attcgttcct atccaaattc
    19741 gacctttggt atcaaaaagc atcgcattca cttggcgcga gggtaacgct ttgccgatgg
    19801 gttttgactt atcctgatgg gtaaatacgc ccgtggtttt atcaagcaca tccaaaccgc
    19861 cggatgtagc caaccacaaa ttaccggacg catcttcagc gatgaagttt acacgattac
    19921 tgttgatgga gttcgaattt ttagagtcat gacgatatat gacaaatgat tctttatcac
    19981 gatcaaaacg acacaaccct ccgtcggccg taccgatcca taataaaccg ctacgatctt
    20041 tgaaaacctt gagaatacgg ttatccgaga gtgacgccgt atccaaatgg ttgtttttgt
    20101 aaacggtaaa attgtaaccg tcgtaacgat tcagaccatc ctgtgtacca aaccataaaa
    20161 atcctcggct atcttgtacg atgctatata ctacggtttg cgacaacccc tgctctatcg
    20221 aaagcgtctc aaacgtaatg ttttgttgtg cgtgcagagc tgacaaccaa aacagcaccg
    20281 taccaataaa aaaccgacga atcatgtaga gtgcctttaa aaattaaatc atggttttag
    20341 atttttcgac accggaggcc actgttttat acagaagttt agtgggacaa gacttggtaa
    20401 gaaattcgtt gataacctta gacgcgatca ttttgatcat gtctttttcc atttcggaat
    20461 agccggagta gatgatctta tagtattgct tatcgccatg ctcttcttta actttttg
//
LOCUS       DAOQGF010000058        19931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1259_length_19931_cov_8.521835, whole genome shotgun sequence.
ACCESSION   DAOQGF010000058 DAOQGF010000000
VERSION     DAOQGF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19931
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1259_length_19931_cov_8.521835"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK937_09280"
     CDS             complement(<1..280)
                     /locus_tag="PK937_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32804.1"
                     /translation="MSGEIVQKQKEALLKKIAEHKALSKPVLDKVRKASSLEVVIMFD
                     TTGSMDTYLSEVQVHVQLIVREIRKRVPDARLSVIVYKDHEQGPNVTKT"
     gene            587..1084
                     /locus_tag="PK937_09285"
     CDS             587..1084
                     /locus_tag="PK937_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNH32805.1"
                     /translation="MTEEPKKDEIHKAFRAYLISHSLKATPERFAILDEIYATNFHFD
                     ADDILMRMRKKNSMASRATIYRTLELLEKCGLIRKAKLGDTKAYYEHTYGRHHHEHMK
                     CTSCGRVIEFESEEIEKLQDVICRQYNFKMTHHILHLFGICEECQQSPDRKPKQTKLS
                     ELTVK"
     gene            complement(1094..2170)
                     /locus_tag="PK937_09290"
     CDS             complement(1094..2170)
                     /locus_tag="PK937_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNH32806.1"
                     /translation="MRFEVLPAHNPAVRERWNAFASSVDGSSVLQSYEWGEVKRGLWQ
                     PFYTAVTDETGQIILAALILKRPMPLVGRAIFYAPRGPLFRELNAALLKAFADGVCAL
                     AKKENVFALRCDPEIEETNTSILSVFQNAGFSRNPENIQPRGTILLDIRPGEDDLLKS
                     FHHKTRYNIKLAEKKGVTVEEINSSDGIDIFYDLFEITSARDKFMILRRSYFQHLHKT
                     LSDSGLSTVFVAKYDGKPLGAVILTTFGKRMVYLYGASSNAHRNLMPNHLLHWRAILW
                     AKERGLTTYDFWGVPANPTETSPLWGVYRFKKGFNETETRWVGTYELVFNRFWYFVFE
                     KAAVAFKTAIRFLKTGKIKSSVGD"
     gene            complement(2178..2579)
                     /locus_tag="PK937_09295"
     CDS             complement(2178..2579)
                     /locus_tag="PK937_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNH32807.1"
                     /translation="MKPGSGPIVFFDGVCNLCNHAVDFIIRHDTPHRIRYASLQGETA
                     ASHLPLEIRSSLSTLVLFHKEQIFTHSTAVINIARLMGWPWKMAGVFFIIPRFLRDAL
                     YTVIARHRYLWWGRRPTCRIPSVEERQLFLP"
     gene            complement(2576..4360)
                     /gene="carB"
                     /locus_tag="PK937_09300"
                     /pseudo
     CDS             complement(2576..4360)
                     /gene="carB"
                     /locus_tag="PK937_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(4554..5096)
                     /locus_tag="PK937_09305"
     CDS             complement(4554..5096)
                     /locus_tag="PK937_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32808.1"
                     /translation="MQRIKLGLTLALLSFLIACEEDPATQVERGWQLFESGDFLEARS
                     AFMNAATSGSTDAYNGLGWSYLRLDSISEARIAFAFASSDSNPEIYAGWSSANVLSED
                     FQKALEKSAVVLRQDANFVFSHDTDVDYKDMIWQRAYCYYHLGQYALSLQEIQKISPD
                     FNSVNPSDILNKLESLSNEL"
     gene            complement(5117..13372)
                     /locus_tag="PK937_09310"
     CDS             complement(5117..13372)
                     /locus_tag="PK937_09310"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNH32809.1"
                     /translation="MKTLLTMIALIFSASDMYSQTALTGGITQSGNFTAAGQTATYSY
                     NQSVSYWSVVGIRPPSTSDFDIELSANTNFSPTLASSTLGTGKVDFIVADYNHSPTGA
                     QYVRLKDYSGAGTYYVEYESGAHTLGVPSNSGTLSWTSNEIVKVWDVYLQAGVTYTFK
                     LRTLSGLQDYGISLYKSNTAAYYAGRSSAAATSDITASGTDEYFSYTPSVTDYYGFVV
                     WSNDNYGTSGTYDITVSTLATNNPLSSAASPGQFSYYQTNTNWSVVALRSGSGANDNL
                     SLYTNSSYTTLATQSALASGIEIIATENDTITQGYFRYPKVDRTGGTSQYTLEWDESS
                     GYIGLGNYTTTAWNANTVAKVYNLSMSQGAKYRIKLHTVSGSVDAGLLLFKPNGSLSS
                     QARSAALQSADANGAGLNEEIILTAPVSGSYALVVYSNTATAGSFDLSFNQEILAASN
                     VYGQGVKHYAFDQQNAAWSVVGVRPPSNGNADIVLYNNHTYTTPLATSNTAGTGQVDF
                     VVGDQHHTAKGIYYVSTDDPVNPSRVNYTTQWEGGNESLTAPGTNSGYAWAKTDVVKI
                     WDVQLVSGINYTFNLSVSGGTPNLGMAIFRSSSTTESFYFGRSGAAAIADVNGNGGGE
                     SFSYTPSGALGTLDHYGIVVWSNDTTSAGSVRIDIAQPLMTSATPVSASSSPGLYAFN
                     QSVSGWGVIALRPQSFEDFDLELHGASDYNNLLAQSTLGTGAVEFIAGDFRAGHAPLK
                     TYFTKAVQNSGKGPYAIEWEGTNETLPVEGNSGTISWGTTAIAKVYNVPLVGGRTYKI
                     KLDFLTNAFDAGMALFQPSTGNNFFPRGSQAMLADVAGAGNESMTFTAPMTDTYGLVV
                     WGNTQPASAATFAIDINQPPVVSVPIPDVNFSEDQGSGLVANSNLNGNFSDIQPLSYS
                     ASVLKGGGITASVAGSTLKVSTVSDSVGQYPVVVTANDGTFFTSDTLIVTLAPVNDLP
                     YRSALIPDPKIAEDEQSVFASKLSGYFTDIDNPVLSFGVTALNSGVSAYVSNDSLYLS
                     GQQDFNGNANLRISATDGSFSVYDTINVTVTPVNDVVVLSQTIADTTLPEDFSGLVFI
                     RKLSNIFQDIDNDSIAFRATVTGGAIGAVIQHDSLYVTSSPDFYGQSTISILASDGSS
                     RDGLGKLSDYFSESANTVETSFRVTQSPVNDPPMRSNPIVDKTYAENFNKVLVQKLSL
                     VFTDIDNSSLTYGVSTISKDQDVFGFISNDSLYVLSSKDSNGVDSIRVTASDGMFTAA
                     DTFRVNVTNVNTAPTVTSAINNVTVSEDFGKNFVRLLTTVFSDIDGNALSYSVSVLPG
                     DNDVTAMISNDSLYILSSVDSSGGDTLRVTASDGQYSVQTQFTVTVNPVNDGPKIISA
                     LRDTTYNEDFPKTLVRRLSSVFLDADHPSLTYAGSVLSGGASVVVSNDSLYATGTLNA
                     NGTAAIRVSATDGTLSVADTFQINIKPVNDAPVFSKTVADTSLLEDFGKVFYRKLSTH
                     VSDVDNASLTYNAYVLSGSIQVQISTDSISLISQADYYGAATIRWTASDGSYTLADTF
                     TVNIANVNDGPVRVTALRDTVFSEDFGHVFVRQLGTVFSDVDNAALIYTATVLSGNAT
                     TAIPSGKDSLYIQSSTDFNGDLIIRTTASDGALSVSDTFTVTVSPLNDGPYRTVNISD
                     ISVQEEFSKTYVAKLSQHFSDNDDASLSYAVINNPSSFDAMIGGDTLYITSRPDSNGV
                     YSLVVRTKDASLSAYDTLTVTINNVNDAPFVATPLPDTDVDEDFEKYFVADLQQIFSD
                     IDNSTPAYSVTSLTPSVVQAQVSGDSLYLISVPAVYGSGQIRVTADDGQASAADTITV
                     TVNNVNNSPYVANVLRDTVLQEDFGKVFLVTLSSHFSDIDNVSLQYSARALQNGLKAQ
                     IHQDSLYVLSDADYYGIVEVEITAADGYLASLDTMQIVLTNTNDAPYMAASAPDIVLN
                     EDFGTWTYGNAGTYFADIDNALTYGVEVLSAGVNAAVFGDTIKLYSTARFYGTSQIRL
                     FATDGQFTISDTMAVQINNVNNTPYVAASVPDTTILEDFGYTWIRTLSPYFQDYDNAT
                     LTFAATALTPGVSVETGHDTLYIQSQSDFFGSVAIKLSASDGEYTTTDTMAITIQNVN
                     DSPALSHPMRDTSLAEDFGKTALYQLTDIFSDIDNGSLSYGSEALAAGVTGSISGDTL
                     YVHSTPYFNGDADVRIFATDGEYQVSDTIRISVTGVNNAPYRLAALPDTSLPEDFGKM
                     PYRVVSQFFSDVDNMTLTYSAQVLQGQIGVQTVNDSLYFISVQDFNGAASVKVTASDG
                     FFTVHDTLVLTVTPMNDAPASALPLQPGPSVLTNNLRPTFNWHASKDIDGDAITYTLT
                     VARDNAFSDVILTQQSTDTVATASADMDTTAVLFWRITANDNHGGVVIGPATSFSTDA
                     RKAFFQIGVLANTVSPLHVGLYVRSTKQLNGNPAGQFELSENGNVISAHNDTFEKLTA
                     HTEPTYYTNYVIRQSGTLKISVTGTDMVGNVTVAQRNYAVDVLARNKPFATASDGIVW
                     SGTAMSEGMTAIAREDADAGPMNKNTSASLYTDEAISPVYEMIVTADVKGKLTLTYSD
                     DVISNLHRIISGEVDERLIGIYAEENGQWVYIGGQGKNGSVTTSWNKSGKIMARYNPA
                     HTIIPDHVELLQNYPNPFNPSTTIRFGLSENGTVRLTVYNLLGQKVKTLVQQHVAAGY
                     HTIVWNGRNESGHAVSSGVYLYRLEIQGHVVTKKMMFLK"
     gene            13721..14185
                     /locus_tag="PK937_09315"
     CDS             13721..14185
                     /locus_tag="PK937_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH32810.1"
                     /translation="MLLTKSCLYGIRAAMYVASLGHKEYVPIQQISENLNISFHFLTK
                     ILQQLTENNIMISYRGPKGGVALARDPKDISLTDIVEAIDGPNIFDQCIFGLPGCGNL
                     APCPLHSEWAKHREKINAMFENATLNTLADKINTFNFRIADEGYEEEKKQPK"
     gene            14249..14995
                     /gene="ric"
                     /locus_tag="PK937_09320"
     CDS             14249..14995
                     /gene="ric"
                     /locus_tag="PK937_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNH32811.1"
                     /translation="MKSFEDMPLGQIVLEYPRSAEILEHYRLDFCCGGKKILSDACRD
                     KDIALADVCRDIEKAIQSPSQGQQPKPIHEQPAGFIVEYIQSNHHTYMRHAIPLIERH
                     LNKVAQVHGDHHPEMVTVREAFEALSSELKLHLQKEEVILFPYVCELEDHITHHAPIR
                     PNGFGTVLNPIHVMENEHAFAGQQMEKIRTLTADYHVPVDACTTYRLLLHELQEFEQD
                     LHKHVHLENNVLFPKAVMMEKQIFNKTSHN"
     gene            15021..15524
                     /locus_tag="PK937_09325"
     CDS             15021..15524
                     /locus_tag="PK937_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNH32812.1"
                     /translation="MKSYVLALAIFAATISMTACGSSNEEAKASWGEAANKPAKPDPY
                     AGLTAFEKEHGIGPVKEELKLEAINTTLVKKGEKAFETKCYACHRLDTKLVGPPLQEV
                     LKTRKPEYVMNMMLNPEGMIKNHPDAKKLFAQFLIPMANQNLTMDECRAILEYLRHAN
                     ENAKKPS"
     gene            15594..17558
                     /gene="nosZ"
                     /locus_tag="PK937_09330"
     CDS             15594..17558
                     /gene="nosZ"
                     /locus_tag="PK937_09330"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNH32813.1"
                     /translation="MFRKPSARIVYASLVVLALAAVWIGCPAKKEATIGSGDAASKVF
                     VPPGSYDEFYAFLSGGFSGQVSVYGLPSGRLFKTIPVFAVNPENGWGFNEETKGMLQT
                     TDGFIPWDDSHHPELSMTDGTPDGRWLFINANNSPRIARIDLGTFRTEEIIEIPNSGG
                     SHASPFTTINTEYVVSATRFSVPTPQADVAINSYKQNFKGAISFVKVDPKEGHMDLAF
                     QILVPGFDYDLSHAGKGPSHGWFFFSTYNTEQAHTLLEVNASQNDKDFIMAVNWKKAE
                     EYIKQGKAKSWTSEYYINRYDDNKHTTISEKKKQVMMLDPKDCPDMLYLMPCPKSPHG
                     ADVDPSGEYIVGGGKLATVIPVFSFSKMIKAIEGKAFDGDRDGIPVLKYDAVLAGEVQ
                     EPGLGPLHTEFDGKGYAYTSHFVSSEVVKWKLGTWEVVDRMPTYYSIGHLMIPGGDSK
                     QPWGKYVLAMNKITKDRYLPTGPELTQSAQLIDISGDKMRMLSDFPTIGEPHYAQGIA
                     ADLVRKHEVKIFDINKNEHKYATKSEKDAKVVREGKEVHVYMTASRSHFVPDNIEGVK
                     VGDVVYFHMTNLEQDWDVPHGFAIKGNNNAELLIMPGETATVRWEPKTPGVYPMYCTD
                     FCSALHQEMQGYVRVSAAGSNTALTFSTGK"
     gene            17619..18245
                     /locus_tag="PK937_09335"
     CDS             17619..18245
                     /locus_tag="PK937_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32814.1"
                     /translation="MNKRQKILIVVASLMLTGIYFTPLWKITLDAPQYPEGLGLYIWV
                     NKISGENQHDLRTINGLNHYIGMKEITPESIPELKIMPYFVGFLIITGFIVAFVGNRK
                     VLGVWAFIFFAVLIAGLIDFYLWEYDYGHNLNPHAAIKIPGMSYQPPLIGSKQLLNMH
                     TTSLPSIGGILAGLSFLITLYVLFVSRKNTSITEEKNHVAVKPITGAA"
     gene            18211..18669
                     /locus_tag="PK937_09340"
     CDS             18211..18669
                     /locus_tag="PK937_09340"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNH32815.1"
                     /translation="MLRSNRLPALLSLLSVLIGSCTSGPEPIAYGQDECTHCRMGITD
                     RRFGSEWVTEKGKIFKFDSIECLVKYYYQQKSANDQGTVWVTAFDQPEAFIPAKDAHY
                     LRSEKRPSPMGMFLSAYNGNAAAENAQKENGGDIMSWEQLKVYIEKENRF"
     gene            18752..>19931
                     /locus_tag="PK937_09345"
     CDS             18752..>19931
                     /locus_tag="PK937_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNH32816.1"
                     /translation="MKHNVIIEPFRIKMVEPIYMTSEEERQQLLHQAHYNPFLLKSEH
                     VLIDFLTDSGTSAMSHKQWAAMISGDESYAGAQSWERLESVVKDLTGYPHILPTHQGR
                     AAERILYGHLGGKGKVFLSNTHFDTTRANIEFSGATAIDCPIPEALEPGKPHPFKGNI
                     DLVQVEYWIKKHGAENIAAVILTVTNNTGGGQPVSLANARALSELCAKYKVLFIIDAC
                     RVAENAYFIKHGEAECAHLSYKEIAQAMFDLADGCVMSAKKDGLVNIGGFLALRDDAI
                     AAACTNLLIITEGFTTYGGLAGRDMDAMAVGLQEVFDADYLKYRIRSTQYLGEKINAL
                     GVPIMLPVGGHAVYINAKDLYPHIPVNQYPGQALVCELYKVGGIRCVEVGSVMFGKYD
                     A"
BASE COUNT         5394 a         4538 c         4774 g         5225 t
ORIGIN      
        1 gggttttggt aacgtttggc ccctgctcgt gatctttata aacgataaca gataaacgtg
       61 catcgggtac tcgtttacgg atttcacgca cgatgagctg gacatgaacc tgcacttcgg
      121 acaagtacgt gtccatactg cccgtcgtat caaacataat caccacttca agcgaactgg
      181 cttttcgcac tttatccaaa acaggcttgg atagcgcttt gtgctctgcg atctttttta
      241 agagcgcttc tttttgtttt tgtacgattt cgccgctcat agctgcattt gaagtgttcg
      301 ggaaatgatt ttcgaatata cacgaaaagc catgcacaga caaacaaaaa ttttaagata
      361 aaatcgtctc atggagcctt aaaaaatcgg caacacgttc ttttttttat ccgatgacgc
      421 cttcgaaagg cctcgtatcc aaaaaattgt aaaacctgta ataaacggaa aaaaccgcga
      481 accggtattg aactattgca gaaaaattat tataaaaggg catgttgcgg gtatactatc
      541 aaacacgata cgagaatcag acaagaaagc gtcattcatc gtgcatatga cagaagaacc
      601 taaaaaagac gaaattcaca aagcgtttcg cgcctacctg atttcgcatt cattaaaagc
      661 tacgcccgaa cgttttgcga tcttagacga gatatacgct accaatttcc attttgatgc
      721 cgatgacatc ctcatgcgga tgcgaaaaaa aaatagcatg gcttcgcgtg caacgatata
      781 tcgaacctta gagcttctag aaaaatgcgg attgatacgc aaagccaagt taggcgatac
      841 caaagcgtat tatgaacata cttacgggcg ccaccaccat gagcacatga agtgtacgtc
      901 gtgcggccgc gtgatcgaat ttgaatcgga agagatcgaa aaattacagg atgtgatttg
      961 ccgtcaatat aatttcaaaa tgacgcatca tattttgcat ctattcggta tctgtgaaga
     1021 gtgtcagcaa tcgccggatc gcaagcccaa acaaaccaaa ctttccgaac tcaccgttaa
     1081 ataaaacgtt tttttaatcc ccgaccgaag attttatttt tccggttttc aaaaatcgaa
     1141 tggcggtttt gaatgccaca gcagcttttt caaagacaaa ataccaaaat cggttaaaga
     1201 ccaattcgta cgtacccacc cagcgcgttt cggtttcgtt gaatcctttt ttaaaacggt
     1261 acacccccca gagcgggctg gtttcggtcg ggttggctgg aacgccccaa aaatcgtatg
     1321 tcgtcaaccc gcgctcttta gcccaaagaa tcgcgcgcca atgcaataaa tgattaggca
     1381 taaggttgcg atgtgcgttg gatgaagcgc cgtataaata aaccatgcgt tttccgaagg
     1441 tagtcaaaat cacagcgccc aaaggtttac cgtcgtactt ggcaacaaat acagtactta
     1501 aaccgctatc ggaaagtgtt ttatgtaagt gttggaaata cgaacgccga agtatcataa
     1561 atttatcccg cgcactggtg atctcaaata aatcgtaaaa aatatcaatg ccgtcggaag
     1621 aattaatctc ttctaccgta acgccttttt tttccgctaa tttgatattg tatcgagtct
     1681 tgtgatgaaa ggatttgagc agatcgtctt caccgggacg aatatccaga agaatcgtac
     1741 cgcgcggttg gatattctcc gggtttcttg aaaatcctgc attttgaaaa acagataaaa
     1801 tcgaagtgtt ggtttcttcg atttcaggat cacatcgcaa tgcaaaaaca ttttctttct
     1861 tagctaaagc gcagacaccg tcggcgaatg cctttagaag tgcggcgttt aattctctaa
     1921 aaagcggtcc gcgcggtgca taaaatatgg cgcgaccgac aagcggcatt gggcgtttga
     1981 gtatcagtgc cgcaaggata atctgaccgg tctcgtcggt aaccgccgtg taaaacggtt
     2041 gccataaacc gcgttttact tcaccccatt cgtaactttg taaaacggac gaaccgtcaa
     2101 ccgatgatgc gaaagcgttc catcgttcac gtacggcagg attatgggcg ggtaatactt
     2161 caaatcgcat gtatgactca aggtagaaat aattgacgtt cttcgaccga cggtatcctg
     2221 cacgttgggc gacgtcccca ccacaggtaa cgatggcgtg cgatgaccgt atagagcgcg
     2281 tcacgcaaaa agcgagggat gataaaaaaa acgccagcca ttttccacgg ccaacccatc
     2341 aaacgggcaa tattgataac ggccgtagag tgcgtgaata tttgttcttt gtgaaataaa
     2401 accaaagtcg aaagcgagga tcgtatttcc aagggtagat gtgatgccgc cgtttcgcct
     2461 tgcagggaag cgtatctgat gcgatgcggt gtgtcgtgcc tgattatgaa atccacagca
     2521 tgattacaca aattacatac cccatcaaaa aaaacgatcg gcccggagcc cggcttcata
     2581 ccagcgccgt atgatgttct tggatgctgc gcacatctag cttttgttct ttgatggcgc
     2641 gcaacccttt gacaagcccc agggcgcccg gcaacgtcgt gatcatcggt attttgtaca
     2701 gcatggccgc ttcgccgatg tagcgttcgt ctaattttgc ggctttgcct gtcggtgtgt
     2761 tgatgacgat gtgtatttct ttgtttttaa tgcggtcaac tacgttggga cggccttcat
     2821 gaattttgaa tacatgttta cattcgatgc ctgattgttc cagaactttg cccgtgcctt
     2881 cggtggcata aatcgtgaat cccagatcca tgaattcttt ggcaatcaga gaaattttag
     2941 atttgtcccg gtcgttgaca gtgacaaaaa ctccgcccga cgtaggcatt tgcataccgg
     3001 cggcaatgtg cgctttagct aaggcttgag gaaaagtttc ggcaattccc attacctcgc
     3061 cggtggaacg catttccggg ctcaggtaca tgcgaatacc ggggaacttg gcaaaaggaa
     3121 agaccggatc tttgacggag gtaaacggcg gaataatttc gtttgtgtaa tttaatgatt
     3181 ctaacgtttt tccaaccatc actttcgcgg ctagtttagc caagggtacg ccgatcgctt
     3241 tactgacaaa gggtatggta cgcgaagcac gcggattaac ttccaataca tatacttctt
     3301 cacctttgag tgcgtattgg atattgatca gtccgatgac attcaattcg cgtgcgagct
     3361 gaactgtgta ggctctcagt ttggccagta cttcgtccga agccgaatac ggcggtagaa
     3421 cgcaggagct gtcaccggaa tgaatgccgg cttcttcgat atgctgcatg atgccgccga
     3481 tcactacggt ttttccgtcg gaaatggcat ccacgtcaaa ttcgtacgcg tctaccagaa
     3541 atttatctac gagaatcgga tgctccggcg acacgttgat ggctcgtgcc atgtagtttt
     3601 ccagtgcggt atcatcataa acgatttcca tagcacgacc gccgagaaca tacgaaggcc
     3661 ggactaatac ggggtagccg atgcgatggg cgatcgtgcg ggcatcttca aacgaagacg
     3721 ccgtgccgta atccgggcat ttgatgttta gacgattgag taaggcgccg aattttttac
     3781 ggtcttcggc gacatcaata tcttccggtg atgtaccaag aatttttaca ccggcttgca
     3841 tcagaggtat ggccaacttg agcggcgtct gtccgccaaa ctgcacaatc acaccgtcgg
     3901 gtttttcgga ttcgataata ttcatcacat cttcaaatgt cagcggctca aaatacaaac
     3961 gatccgatgt atcgtaatcc gtgcttacag tttccggatt gcagttgacc atgatgactt
     4021 cataaccttc ttcctgcaat gcatatacac catgtacaca acaatagtca aactcgatac
     4081 cttgtccgat acggttgggt ccgccgccaa gtatgatcac cttcttttta tccgacggcg
     4141 cgttttctgt ttcctgatcg taggtggaat aaaagtaagg ggtctccgct tcaaattctg
     4201 ctgcgcaggt atctacggtt ttgtatacgg gaaatacctt caattcgtgc cgcaaccggc
     4261 gaacggtttt ttcgtccgtt tcccagatgt gtgccaactg acgatcggaa aatccttttt
     4321 gtttggcttc gatcagaagc gcgcgggaga ttttttggat caatatttca agagatgtca
     4381 aggggtacct gcttttagtt agaccggcgc aaactaaaga taacgagcct tcatttcaag
     4441 ggattttata atctgtgcgg aatgagccga tagggcgtat catagaaaaa aaccggtaat
     4501 ttgggcctga gggttgatct ccaaagcatc aaattaccgg tttctaaaaa aagttacagt
     4561 tcgttcgaca gggattctag tttattcaga atatccgaag gattgacgga attaaaatcc
     4621 ggacttattt tttgaatttc ctgaagactc agggcgtatt gtccaagatg ataataacaa
     4681 taggcgcgtt gccagatcat atctttatag tccacgtcgg tatcatgtga aaaaacaaaa
     4741 ttagcatcct gcctgagcac gacggctgat ttttcaagag ccttttgaaa atcttccgac
     4801 aatacgttgg ctgatgacca accggcgtaa atttccggat tggaatccga tgacgcaaaa
     4861 gcaaacgcga tgcgcgcttc tgaaatcgaa tccagccgta gataagacca cccgagtccg
     4921 ttgtaagcgt cggtgcttcc gctagtcgcg gcattcataa atgccgaacg ggcttccaga
     4981 aaatcgccgc tttcaaataa ttgccatcca cgttcgactt gtgttgccgg gtcttcttcg
     5041 catgcgatca aaaacgaaag aagcgccagc gttaatccta attttatgcg ttgcatagat
     5101 atgtatcctt aaatttttat ttcaaaaaca tcattttctt tgttacgaca tgcccctgta
     5161 tttcaagacg atacaggtac acgccgctgg atacggcatg accggattcg ttgcgtccgt
     5221 tccatacgat ggtatgatat ccggcggcca cgtgttgctg caccaaagtt tttacttttt
     5281 gcccgagaag attatacacc gtcaaacgta ccgtaccgtt ttcagataat ccgaaacgaa
     5341 tcgtcgtgga cgggttaaac gggttggggt agttttgcaa taactcaaca tgatccggaa
     5401 tgatcgtatg agccggatta taccgagcca tgattttacc cgatttgttc catgacgtcg
     5461 tcacggatcc gtttttccct tgaccgccga tataaaccca ttgtccgttt tcttcagcat
     5521 aaatcccgat caatcgctca tccacttcac cggaaataat acgatgtaag ttcgaaatca
     5581 cgtcgtcgct gtacgtgagg gttagtttgc cttttacatc cgccgtaacg atcatttcgt
     5641 aaacgggact gatcgcttcg tccgtgtaca aggaggcgct tgtattttta ttcattggcc
     5701 cggcgtctgc gtcttcacgt gcgatagccg tcataccttc ggacatcgcc gtgcctgacc
     5761 aaacaatgcc gtcggaagct gtagcgaatg gtttattgcg tgcaagtaca tctacagcat
     5821 aattacgctg tgcgacggta acgttaccta ccatatccgt tcctgtaacg gaaattttaa
     5881 gagttccgga ttgccggatc acatagttgg tataataagt aggttcggta tgcgctgtta
     5941 atttttcaaa cgtatcattg tgtgcagaaa tcacattgcc gttttctgaa agttcaaact
     6001 ggccggcagg gttcccgttt agctgtttgg tgctgcgcac atatagacct acgtgcaggg
     6061 ggctaacagt attggccaga accccgattt gaaaaaaggc cttgcgggca tcggtgctaa
     6121 aacttgtcgc cggtccgatc actacgccgc catggttatc attggctgtg atacgccaga
     6181 agagtacggc tgtcgtatcc atgtcggccg aagcggtcgc aacggtgtcg gtgctttgct
     6241 gagttaaaat cacatcgctg aaagcgttgt cacgcgcaac ggttaatgtg taggtgatcg
     6301 catcgccgtc aatatctttt gaggcgtgcc aattaaatgt cgggcgcaga ttattggtca
     6361 gcactgacgg accgggctgc aaaggtaaag ccgacgcggg cgcatcgttc ataggcgtca
     6421 ccgtcaatac caatgtgtca tgcacagtaa aaaatccatc cgaagcggtt actttgacgg
     6481 atgcggcacc gttgaaatcc tgtacggata tgaaataaag actatcgttg acggtttgca
     6541 cgccgatctg gccttggagc acttgcgctg aatacgtgag cgtcatatta tccacatcgc
     6601 tgaagaattg cgatacaacg cggtaaggca tctttccaaa gtcttcagga agtgatgtgt
     6661 ccggcaatgc ggcaagacga taaggcgcgt tattcacgcc ggtgaccgag atccgaatcg
     6721 tatcagaaac ttgatattct ccgtccgttg caaaaattcg tacgtccgca tcgccattga
     6781 aatacggtgt gctgtggaca tagagtgtat caccgcttat gctacctgta actccggctg
     6841 ccagtgcttc actgccgtac gacaaagatc cattatctat atcagaaaaa atgtcggtca
     6901 attggtataa agccgtttta ccgaaatctt ctgcaagact cgtatcacgc atcggatggg
     6961 aaagtgccgg gctatcgtta acattttgaa tggtgatagc cattgtgtct gtagttgtat
     7021 attcaccatc gctcgctgat aacttaattg cgaccgagcc gaaaaagtcg gattggcttt
     7081 ggatgtacag cgtatcatgg cctgtttcga cacttacacc gggcgttagt gctgtagcag
     7141 caaatgtcaa cgttgcatta tcgtagtcct gaaaataagg gctaagagta cgtatccaag
     7201 tataaccgaa atcttcgagt atcgttgtat ccggaacgga ggccgctaca tacggggtgt
     7261 tattcacatt gtttatttgt accgccatcg tgtctgaaat tgtgaattgt ccgtcggtcg
     7321 caaataaacg aatttgagag gttccataaa agcgagcggt gctgtaaagt tttatggtgt
     7381 ctccaaacac agcggcattt actccggcgc ttaaaacttc aacgccgtag gttaacgcat
     7441 tgtctatgtc ggcaaaatag gttccggcgt tgccgtaagt ccatgtgccg aaatcctcat
     7501 tcaaaacgat atccggtgcg gatgccgcca tgtatggtgc gtcattggta ttggtcaaaa
     7561 caatctgcat cgtatcaagc gatgccaagt atccgtcggc ggctgtgatt tccacctcga
     7621 cgatcccata ataatccgca tcactgagca cgtacaaact gtcttgatgg atctgcgctt
     7681 ttagtccgtt ttgtaaagca cgtgccgaat attgtagaga tacattatca atatctgaga
     7741 aatgcgagga aagcgttact aaaaagactt ttccaaaatc ttcttgtaaa accgtgtcgc
     7801 gcaaaacatt tgcgacatag ggcgaattat tgacattgtt tacggttacg gtgatcgtgt
     7861 cggccgcgga ggcttgcccg tcgtcggcgg tcacgcgaat ctgtccggag ccatatacgg
     7921 caggtacact gataaggtaa agcgaatcac ccgaaacttg cgcctggact acagatggcg
     7981 tcaatgaagt aactgaatac gcgggcgtgc tattgtctat atcgctgaaa atttgttgga
     8041 ggtcggctac aaagtacttt tcaaaatctt catccacatc ggtatccggc aacggcgtcg
     8101 caacaaacgg cgcgtcattt acattgttaa tggtaacggt aagcgtatca taggctgata
     8161 aggaggcgtc tttggttcgg acaaccaaac tatatacccc gttactgtcc gggcgacttg
     8221 tgatgtaaag cgtgtcgccg ccgatcatcg catcaaatga tgacggatta ttgatcaccg
     8281 cgtaactcag cgaagcgtcg tcgttgtcac tgaaatgctg gctaagttta gcaacatagg
     8341 ttttggaaaa ttcttcctgt accgaaatat cggaaatgtt aacggtacga taaggtccgt
     8401 cattgagcgg ggatacggta acggtaaatg tgtcgctgac cgacaaagcg ccatcggaag
     8461 cagttgtgcg aataatcaga tctccgttaa aatccgtact gctctgaata tataaactgt
     8521 ctttgcctga tgggattgct gttgttgcat ttcccgataa gaccgttgcc gtataaatga
     8581 gcgccgcatt gtctacatca gaaaacaccg taccaagttg tcggacaaat acatggccga
     8641 agtcttcgct gaaaacggtg tcacgtaaag cggttacgcg aaccgggccg tcatttacgt
     8701 tagcgatatt gactgtaaaa gtatcggcca gtgtatacga tccgtcgctt gcggtccagc
     8761 ggatggttgc cgcgccgtaa taatcggctt gggaaattag tgaaatcgaa tcggttgata
     8821 tctgaacttg tatactgccg cttaatacgt acgcattgta ggttaatgaa gcattgtcta
     8881 catccgagac atgcgtactc agtttgcgat aaaaaacttt tccgaaatct tccaagagac
     8941 tggtatccgc gacggtttta gaaaacacgg gtgcatcgtt gacgggtttg atattgatct
     9001 gaaatgtatc cgctacgctg agtgtgccat ccgtagcgct tacgcggatc gccgctgtgc
     9061 cgttggcatt cagtgttccg gttgcataca aactgtcatt tgaaacaaca accgatgcgc
     9121 cgcccgacaa tacggatccc gcatacgtca atgagggatg atcggcgtcc agaaaaactg
     9181 acgataaacg gcgaactaaa gtttttggaa aatcctcatt gtaggtcgta tcacgtaagg
     9241 ctgaaataat tttgggtccg tcgtttacag gattgaccgt aaccgtaaat tgcgtctgga
     9301 cggaatattg accgtcgctt gccgtgacgc ggagggtatc tccgccggat gagtccacag
     9361 aggaaagaat gtacaatgaa tcattgctga tcatggcggt tacatcattg tcccccggta
     9421 atacggacac gctgtaggat aacgcgttac catctatatc cgaaaacacc gtagttaaaa
     9481 ggcggacaaa gttttttccg aagtcttcgc tgaccgtcac attgttgatg gcggaagtga
     9541 ccgtaggcgc cgtattcaca ttggttacat tgacacgaaa cgtatcggct gcggtaaaca
     9601 tgccgtcgct agccgtgacg cgaatactat ccacgccgtt ggagtcttta ctgctcaata
     9661 catacaagct gtcgttgctt ataaaaccga aaacatcctg atctttggag atcgtggaga
     9721 caccataggt cagtgaacta ttatcaatat ccgtgaaaac gagagatagt ttttgtacca
     9781 atactttgtt gaagttttcg gcatacgttt tatctacgat cgggttggat cgcatcggcg
     9841 gatcattgac gggtgattgg gtaacacgaa acgatgtttc gaccgtattg gcactctcgc
     9901 tgaaataatc actgagtttt cccaatccgt cacgtgagga accgtcgctt gccaggatag
     9961 agatggtaga ttgaccgtaa aaatcaggac tgcttgtgac atacaaactg tcgtgctgta
    10021 tcaccgcgcc gatcgcaccg ccggtaaccg ttgcgcggaa ggcgatcgaa tcattatcga
    10081 tatcctgaaa aatattcgat aatttacgga taaaaaccaa tccgctaaaa tcttcgggca
    10141 acgtggtgtc ggcaatcgtt tgcgataata ccactacgtc gttgacgggt gttacagtca
    10201 cattgatcgt atcatagacg ctaaaagatc cgtctgtcgc tgaaattcgt aaattagcat
    10261 tgccgttaaa atcttgctgg ccgctcaggt acagtgaatc gttggacaca taagctgaga
    10321 ccccgctgtt tagtgcggta acgccaaagg aaagaacggg attatcaata tctgtaaaat
    10381 aaccgctgag ttttgatgca aaaacagact gttcatcttc ggcgatttta ggatctggta
    10441 tcagcgcgga acggtatgga agatcattca ccggcgccaa agtgacaatg agtgtatcgc
    10501 tggtaaaaaa agttccgtca ttggccgtca cgacgacggg atattggccg acggaatcag
    10561 aaaccgtgct gactttgagt gtactgcccg ccacgctggc cgtgataccg ccgcctttga
    10621 gtacggatgc cgaatagctc aaaggttgta tatcggaaaa atttccgttg aggttagaat
    10681 tggccacaag tccgctgcct tgatcttctg aaaaatttac gtcgggaatc ggtacgctca
    10741 ctacgggcgg ctgattgatg tctatggcaa acgtggcggc cgaagccggt tgtgtgttgc
    10801 cccaaaccac aaggccgtag gtatccgtca tgggcgccgt aaatgtcata gattcattgc
    10861 ctgcgccggc tacatcggcg agcatggcct gcgaaccgcg tggaaaaaaa ttattacctg
    10921 ttgacggttg gaataacgcc attccggcat cgaaggcatt ggtcagaaaa tccaatttga
    10981 ttttgtaagt gcgtccgccg accaggggca cattgtatac tttagcgata gccgtggtac
    11041 cccagctgat cgtaccggaa ttaccttcga caggtagggt ttcgttggtg ccttcccatt
    11101 cgatcgcata agggcctttg ccgctgtttt gtacggcttt tgtgaaatag gttttaagcg
    11161 gggcgtgtcc ggcacgaaaa tcgcccgcga taaactctac agcacctgtt cccaaggtgc
    11221 tttgagccaa aagattgttg taatcggaag cgccgtgcaa ttctaaatca aaatcttcga
    11281 aagactgcgg tcgtaatgcg atgacgcccc atccggagac ggattgattg aacgcgtaaa
    11341 gcccgggaga actgctggcg gaaacgggtg tcgcactggt cataagcggt tgggcgatgt
    11401 caatgcggac gcttccggcg gatgtggtat cattgctcca aacgacaata ccgtaatgat
    11461 cgagtgtgcc taatgcgccg ctcggggtat aggaaaatga ttcgcctccc ccgttcccgt
    11521 tcacatcggc gatcgcggcc gctccggagc ggccaaaata aaatgattcc gtagtagaag
    11581 aagagcggaa tatcgccatg ccaagattgg gtgtgccgcc gcttacgctc agattaaatg
    11641 tataattgat tccggagacc aattgtacat cccatatttt tacgacatct gttttggccc
    11701 aggcgtaacc gctatttgtt ccgggcgccg ttaacgattc attgccgcct tcccactgcg
    11761 tggtatagtt tacgcggctc ggattcacgg gatcgtccgt gcttacgtag taaatacctt
    11821 tggctgtgtg atgctgatcg ccgacgacaa aatccacctg gcctgtgccg gctgtattgc
    11881 tggtcgctaa aggcgtggtg taggtatgat tattatacaa aacgatatcg gcattgccgt
    11941 tggatggcgg gcgcacgccg actacggacc atgcggcatt ttgttgatcg aaggcataat
    12001 gtttgacacc ttgcccgtac acgttggaag cggcgagtat ttcctgatta aaagaaagat
    12061 caaatgaacc ggcagtagcc gtattgctgt aaacgaccaa tgcgtacgat ccggaaaccg
    12121 gcgcggtgag tatgatctct tcattcagtc cggcgccatt ggcatcggcc gattgcagtg
    12181 cggccgatcg cgcttgggaa ctcagtgaac cattgggttt gaaaagaaga aggcctgcgt
    12241 ccacactacc tgaaacggta tgcaatttaa ttcggtattt agcgccttgg gacatggaca
    12301 ggttatacac tttagcgacc gtgttagcgt tccatgccgt cgtcgtataa tttcccaaac
    12361 cgatatatcc gctggattcg tcccactcta aagtatattg tgaagtgcct ccggtgcgat
    12421 ccaccttagg ataacggaag taaccttggg tgatggtatc attttcagtg gcaataattt
    12481 cgatacccga ggctaatgcg ctttgcgtag ctaaagtcgt ataggatgaa ttggtataaa
    12541 gcgagagatt gtcattggcg ccgctacctg agcgtaaggc gacaacggac caattggtat
    12601 ttgtttgata atatgaaaat tgacccggcg aagccgcgct ggatagcggg ttattggtgg
    12661 ccaatgtcga aaccgtaata tcgtaggttc cgcttgtgcc gtaattatcg ttggaccata
    12721 cgacaaagcc gtaataatcc gtaacgctcg gtgtatagga aaagtattcg tccgtaccgc
    12781 tggcggtaat atcggatgtg gcggcagccg aactgcggcc tgcataatag gcggcggtat
    12841 tggatttgta aagcgatatg ccataatcct gaaggccgga aagtgtgcgg agcttaaatg
    12901 tataagtcac accggcctgc agatacacgt cccaaacttt gacgatttcg ttggatgtcc
    12961 aggatagtgt accgctattg gagggaacac ctaatgtatg tgcgccgctt tcatattcga
    13021 cgtagtatgt acctgcacca ctatagtctt tgagacgaac gtattgtgcg cctgtaggcg
    13081 aatgattata atcggctacg atgaaatcta ctttgccggt acccaatgta ctactggcga
    13141 gtgtagggct gaaattggta ttggcgctaa gttcgatgtc aaagtcggat gttgatggcg
    13201 gccgaatacc caccacactc caataactaa ccgattggtt gtaggagtag gtggccgttt
    13261 ggccggcggc cgtaaaatta ccggactgcg taataccgcc ggtaagcgca gtttgtgaat
    13321 acatatcgct tgctgaaaaa atcaaagcga tcatcgtgag caaggttttc ataattacct
    13381 caaaagcatg ctaaaaacat gacgttatgt gaacggtgaa aggttccctc aaacagaggt
    13441 ataattgttt atttttaatc aaatttaggg taagtctact cggcacaata tcgtgtgatt
    13501 tagctcacaa atgaggctac ataagcgtgt aggttttgta aaaaatttta aataattcgt
    13561 caataaaatg acgccacccc cgattttttg tattttcaaa aaaaaattga aaaaccagac
    13621 caaaatactt gacaaatgag gttgaatatc ttattttaag acaaaagact ctgaaataca
    13681 ggaatagagc aaagatttat ccggaaaaga aactattacc atgctactaa ctaaaagttg
    13741 tttatacgga attcgtgccg cgatgtatgt ggcgtctctt ggtcataaag aatatgtgcc
    13801 gattcagcag atttctgaaa acctcaatat atcatttcac ttcttgacca agattttgca
    13861 gcagctaacg gaaaataata tcatgatatc ctaccgtgga cccaaaggcg gagtggcctt
    13921 ggcgcgcgat cccaaagata tttcacttac ggatattgtc gaagccatag acggtcctaa
    13981 tatttttgat caatgtattt ttggattgcc cggctgcggc aatcttgcgc cttgcccctt
    14041 gcatagtgaa tgggccaaac accgtgagaa gataaatgcg atgtttgaaa atgctacact
    14101 caatacactg gcggataaaa tcaatacgtt caatttccga atcgccgacg aaggatatga
    14161 ggaagaaaaa aaacaaccga agtgaagcgg ggaatttttt tattcatatt aaagataaaa
    14221 tactcttaaa tcttctaaag gattaaaaat gaaatcattt gaagacatgc ctcttggtca
    14281 aatcgtattg gaataccctc gttcagccga aattctcgaa cactatcgtt tggatttttg
    14341 ttgtggcggg aaaaaaattt tatccgacgc ttgccgtgat aaagatatcg cgctggcgga
    14401 tgtatgccgt gatatcgaaa aagctattca atcaccctct caaggtcaac aacctaagcc
    14461 gatccatgaa caacccgcgg gatttatagt cgaatatatt caaagcaatc atcataccta
    14521 catgcgccac gcgattccac tgatcgaacg tcaccttaat aaagtggcac aggttcatgg
    14581 cgatcaccat ccggaaatgg taaccgttcg tgaagcgttt gaagcgctat catccgaact
    14641 taaattgcat ttgcaaaaag aagaagtgat tctttttccc tacgtctgcg aattggaaga
    14701 tcacatcacg catcacgcgc cgatccgtcc caatgggttt ggcaccgtgc tcaatccgat
    14761 ccatgtcatg gaaaacgaac atgcgtttgc gggacagcag atggaaaaaa ttcgcacgtt
    14821 gacggccgat taccatgttc ctgtggatgc ttgtacgacc taccgtctgc ttctgcatga
    14881 gttgcaagag tttgaacaag accttcataa acatgtgcac ctcgagaaca atgtcttgtt
    14941 tcccaaggcc gtcatgatgg aaaaacaaat tttcaacaaa acctcacata attaatccat
    15001 aaacctcaaa ggaaaatatc atgaaatcct atgtgttagc gctcgccatt ttcgcggcca
    15061 ccatatctat gacagcttgc ggttcatcca atgaagaagc caaagcttct tggggagaag
    15121 ccgccaataa acctgccaaa ccggacccgt atgccggatt gacggcattt gaaaaagaac
    15181 acggtatcgg cccggtcaaa gaggaactga aattggaagc catcaatacg acactggtca
    15241 aaaagggcga aaaagccttt gaaaccaagt gctacgcctg ccatcgtttg gataccaaac
    15301 tggtcggccc gccgctccag gaagtactca aaacccgtaa gccggaatac gtgatgaata
    15361 tgatgctcaa tccggaaggc atgatcaaaa atcatccgga tgccaaaaaa ttgtttgcgc
    15421 aatttttgat tccgatggcc aatcagaatc tcacgatgga cgaatgccgt gccatcttag
    15481 agtatctgcg ccatgctaat gaaaacgcta aaaaaccttc gtgatgattt cgatggctta
    15541 taccatcgtt tacataaaac aaaaaaacga tctgagtgat cgaggaggct cttatgttcc
    15601 gaaaaccatc tgcacgcata gtttatgcgt cgttggttgt gctcgcatta gccgcggtct
    15661 ggatcgggtg ccctgcaaaa aaagaggcaa cgatcggtag cggtgatgcc gcatccaaag
    15721 tatttgttcc acccggaagt tatgatgagt tctatgcatt tctctccggc ggctttagtg
    15781 gtcaggtgtc tgtctatggt ctgccaagcg gtcgcctttt caaaacgatc cctgtttttg
    15841 ccgtaaaccc tgaaaacggc tggggcttca atgaagaaac caaaggtatg cttcagacga
    15901 cggacggctt cattccctgg gacgacagcc accatcctga attatccatg accgacggta
    15961 ctccggacgg acgttggctt tttatcaatg ccaataattc gccgcgtatc gcgcgtattg
    16021 atttgggtac attccgtacc gaagaaatca tcgaaattcc caatagcggc ggcagccacg
    16081 cttcgccgtt cacgacgatc aatacggagt acgttgtatc ggcgacacgt tttagcgtgc
    16141 cgacaccgca agccgacgta gcgatcaatt cgtacaaaca aaatttcaaa ggcgcgattt
    16201 catttgtcaa agttgatcct aaagaaggcc acatggatct cgcgttccag atactggtgc
    16261 cgggtttcga ctatgatctg agccatgccg gtaaaggtcc gtcccacgga tggttcttct
    16321 tctccacgta caatacggaa caagcgcata cgttgcttga agtcaatgcc agccagaatg
    16381 ataaagattt tatcatggcg gtaaactgga agaaagccga agaatatatc aaacaaggca
    16441 aagcgaaatc ctggacttcg gaatattata tcaaccgtta tgatgacaat aagcacacca
    16501 cgatttccga aaagaaaaaa caagtgatga tgctggatcc caaagattgt cccgatatgt
    16561 tgtatctgat gccttgcccg aaatctccgc atggcgcgga tgtggatccg tccggcgaat
    16621 acatcgtcgg tggcggaaag ctggctacag ttattcccgt attttctttc agcaagatga
    16681 tcaaagccat tgaaggcaaa gcgtttgacg gtgatcgcga tggtattcct gttctcaaat
    16741 acgatgccgt attggccggt gaagtacaag aaccgggtct cggaccgctc cataccgaat
    16801 ttgacggtaa aggttatgcg tatacatccc acttcgtatc atcggaagtt gtcaaatgga
    16861 aactcggtac gtgggaagtc gttgatcgta tgccgaccta ctattcgatc ggccacctca
    16921 tgatccccgg cggcgacagt aaacaaccgt ggggcaaata tgtattagcg atgaataaaa
    16981 tcaccaaaga ccgttatctt ccgacgggtc cggagctcac gcaatccgct cagttgatcg
    17041 atatcagcgg cgacaaaatg cgcatgttgt cggatttccc gacgataggc gaaccgcact
    17101 atgctcaagg cattgcggcg gatctcgttc gcaaacatga agtcaaaatt tttgacatca
    17161 ataaaaacga acataagtat gcgaccaaga gcgaaaaaga tgccaaagtc gttcgtgaag
    17221 gcaaagaagt gcatgtgtat atgaccgctt cgcgctctca ctttgtgccg gacaatatcg
    17281 aaggcgtcaa ggtcggtgat gtggtttatt tccatatgac caatctcgaa caagattggg
    17341 atgtgccgca cggatttgcc atcaaaggca ataacaatgc ggaacttctg atcatgcccg
    17401 gtgaaaccgc taccgtacgt tgggaaccga agacacccgg cgtttatccg atgtattgca
    17461 cggatttctg ctctgcattg catcaggaaa tgcaaggtta cgttcgcgtt tcggctgccg
    17521 gttccaatac cgcgctgaca tttagcacag gtaaataagt ttggattttg ggggaggctt
    17581 caaaaaacct cccctttatt ttctttgtaa ggtgtgtcat gaataaacga caaaaaatat
    17641 taattgtggt ggcttcgctt atgctgaccg gtatttattt tacgccgctt tggaaaatca
    17701 cgctggatgc gccgcaatat cccgaaggac tcggtctcta tatttgggtc aataaaatca
    17761 gcggcgaaaa tcagcacgac ctcagaacta tcaatggtct caatcactat atcggcatga
    17821 aagaaattac gcccgaatcc ataccggaac ttaaaatcat gccttatttt gtaggttttc
    17881 ttattattac cggattcatt gtcgcctttg tcggtaatcg gaaagtttta ggtgtgtggg
    17941 catttatatt cttcgccgtg ctcattgcag gtttgattga tttttatttg tgggaatacg
    18001 actacggtca taatctcaac ccgcatgcgg cgatcaaaat tcccggtatg agctatcaac
    18061 cgccgcttat cggaagcaaa cagcttctga atatgcatac gacatcgcta ccgagtatcg
    18121 gaggcattct ggcaggatta tcatttttga tcacgttgta tgttcttttc gtatcacgta
    18181 aaaacacgtc catcacggag gaaaaaaacc atgttgcggt caaaccgatt accggcgctg
    18241 cttagtcttt tatcggtact gatagggtcc tgtacttccg ggcccgaacc tatcgcctat
    18301 ggacaggacg agtgcaccca ttgtcgtatg ggtatcacgg atcgccgttt tggcagcgaa
    18361 tgggttactg aaaaaggaaa aattttcaaa tttgattcca tcgaatgttt ggtgaagtat
    18421 tattaccaac aaaaatcagc caatgatcaa ggtacggttt gggtgacggc atttgatcaa
    18481 cccgaagcat ttattccggc taaagatgcg cactacctgc gcagcgaaaa aagacccagc
    18541 ccgatgggta tgtttctctc cgcgtataac ggaaatgcag ccgccgagaa tgcgcagaaa
    18601 gaaaacggtg gtgatattat gagttgggaa caactcaaag tttacataga aaaagagaat
    18661 cgtttttaaa atatgacttt tgtcagaaaa aaagaagcat taaaatctat attgtgacca
    18721 ttgtaaccga aaaactatca ggaaaaagac aatgaagcac aatgttatta ttgagccttt
    18781 tcgtatcaaa atggttgaac ccatttatat gacgagcgaa gaggaacgtc aacaactttt
    18841 gcatcaagcg cactacaatc cttttttact caaatccgag catgtgctga ttgatttttt
    18901 gacggacagc ggtacgtcgg ccatgagtca taaacaatgg gccgctatga tcagcggcga
    18961 cgaatcctat gccggcgcac aaagttggga acgtctggag tccgtcgtca aagaccttac
    19021 cggatatcct catattctgc ctacgcatca ggggcgcgct gcggaacgta ttctgtacgg
    19081 tcatctgggc ggcaaaggta aagttttttt gagtaatacg cactttgaca caacccgggc
    19141 caatatcgaa ttttccggcg ccacggcgat cgattgcccc atccccgagg cgctggagcc
    19201 gggtaaaccg catcctttca aaggtaatat tgatttggtg caggtcgagt attggatcaa
    19261 aaaacatggc gccgaaaata ttgccgccgt tatcctgacc gtcaccaata ataccggcgg
    19321 cggccaaccc gtgagtcttg ccaatgcccg cgctttgagt gaactctgcg cgaagtacaa
    19381 agttttgttt atcatagatg cctgccgcgt cgcagaaaat gcatacttta tcaaacatgg
    19441 cgaagcggag tgtgcgcatc tttcatataa agaaattgca caagccatgt ttgatcttgc
    19501 cgacggttgt gtgatgagcg ccaaaaaaga cgggctcgtc aatatcggcg ggtttctggc
    19561 gttgcgggac gatgcgatcg ccgcagcttg taccaatctt ttgatcatta ccgaaggttt
    19621 taccacatac ggcggactag ccggtcgtga tatggacgct atggccgtgg gtttgcagga
    19681 agtttttgat gcggattatc tcaagtatcg cattcgcagc acacagtatc tcggtgaaaa
    19741 aatcaatgcg ctcggcgtgc cgatcatgct acccgtcggc ggtcatgcgg tgtatatcaa
    19801 tgccaaagac ctgtatccgc atatccccgt aaatcaatac cccggacaag cgcttgtttg
    19861 cgaactttac aaagtcggcg gtattcgttg cgtggaagtg ggttcggtga tgtttggcaa
    19921 atacgatgcg t
//
LOCUS       DAOQGF010000059        19318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1304_length_19318_cov_6.493277, whole genome shotgun sequence.
ACCESSION   DAOQGF010000059 DAOQGF010000000
VERSION     DAOQGF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_19318_cov_6.493277"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1016)
                     /locus_tag="PK937_09350"
     CDS             complement(72..1016)
                     /locus_tag="PK937_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH32817.1"
                     /translation="MKAFIEMLKLRRYSSNTLGAYEMQLKRLFTHYKDRLPSSLTEHD
                     MLMYIQDVLVPAGVSPSYQNQAINAFKCYFENFLGREKMNLAYQRARVTVRLPVVLSQ
                     DELRGIFASVKNMKHRCILFLIYSSGLRVGEAVNLNVNDIDSRRMLLHIRNGKGGKDR
                     FTILSDQALKLLRDYYKQYRPKKYLFEGVHGGRYSVRSIQQMFTNAASRAGIQKRVSV
                     HSLRHSFATHLLESGVDIRYIQELLGHESSKTTEIYTHVSSRELSKIESPLDRWYTDH
                     PKTGIVREGDPTILNAIAHITRKWRLLRNTHITRLRTL"
     gene            complement(1191..1739)
                     /locus_tag="PK937_09355"
     CDS             complement(1191..1739)
                     /locus_tag="PK937_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32818.1"
                     /translation="MKTTAKLFVLAVLALALSAGSTMAQNPNAEKGKAHKEEKGGKTE
                     KAKKEVKSEDELQPKGKPEGKGSEGNAYGKNKGEMSGKEFGKMRSADAKLKMKEKRKN
                     AHEKITGAEEKIAATEARIKTAKENVEKKKKSGQLKGDKLKEKEEKLKAAEEKIAKLK
                     DLVGKEKDKLTKIPDESAPAAP"
     gene            complement(1761..1970)
                     /locus_tag="PK937_09360"
     CDS             complement(1761..1970)
                     /locus_tag="PK937_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32819.1"
                     /translation="MRKYIMMLAVLALWGIAACGEKKPSAEELDHKEAVRIDSVANDL
                     NASTESLQQQVDETEKAVDDLLKDI"
     gene            complement(2022..3572)
                     /locus_tag="PK937_09365"
     CDS             complement(2022..3572)
                     /locus_tag="PK937_09365"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNH32820.1"
                     /translation="MHTFLSPKPHVVLSKSFSTPFQNAIATARTCYSAKGIVRDEQIT
                     DKHVPLAQSVYQAGHHTVFSHAYFQFELANVSRQFLWTFLHSHPFYNSEQVSQRYVEV
                     KPDTFLIPALEGEALAIYREAIAFQTAAYFKLNEMLRPSAEAAYFKRFPARAKVREKY
                     AKEIQKKCQEISRYVLPVAAFSYLYHTVSGITLLRYYRLCNQMDTPVEQRVVVEQMVQ
                     EMIRLDPMYKAILEEALPIEETPEYLFYQAHPDFYDDAGRKAFAAEFDRSLDGRMSKL
                     VDYKINNEAVVAQSVREVLGLTRERLSDADAIALALDPSKNKLYGESLNLTTMSKITR
                     ALVHAHYTFRKKISHAADSQDQRHRMTPGSRPILTAHRLAEPDYITPALILEDANVHA
                     YYDEVMTRTWEYIARLRAHHVSDENAMYLLPNAVAIRFTESADLQNLHHKMAMRLCYL
                     AQEEIWRASVDETQQIQQVNPVIGNLLLPPCGIRLRAGMRPYCPEGDRFCGERVWTMT
                     LDQYQRVI"
     gene            complement(3625..4398)
                     /locus_tag="PK937_09370"
     CDS             complement(3625..4398)
                     /locus_tag="PK937_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH32821.1"
                     /translation="MQNINYTNDNGIITIGLRRPKVNALNRELLGELSEACDRIRQDD
                     TARVVILRSELDNFCAGADLKERQGMSESDVKKFVRYIIGGTIHKIGDLEVPTIAAVN
                     GSALGGGCELALACDFRIFSEDVKTGLRETALGIIPGAGGTQRLPRLIGYQKALHWIL
                     TARVFTAQEALHDGVAMEVCTLNELHARAQALAEEILANAPIAVRQAKKAVRLGMGTS
                     LQNALEIENECYNDVIPTEDRLEALKAFSEKRKPNWSNR"
     gene            complement(4584..5027)
                     /locus_tag="PK937_09375"
     CDS             complement(4584..5027)
                     /locus_tag="PK937_09375"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH32822.1"
                     /translation="MNEQEIRKIVEEARRQLGAQASDETVKHVAMEAIRRLDGDKGYV
                     QEHHSTFRYLNRTEGRIIVTAFGKNGPGIISNISQTLTDCRCDILDVSQKIMQEFFTL
                     MMLVDIKNAACPFGTIKENLARTSETLGIRILAQHEDVFKAMHRI"
     gene            complement(5068..6297)
                     /locus_tag="PK937_09380"
     CDS             complement(5068..6297)
                     /locus_tag="PK937_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32823.1"
                     /translation="MKKETSDSSRNTRTVYDPYRLNINIKSGLFAVAILMIFGVVLYT
                     QYVVEKLRGDSRHLVRIYTHLIAHIGEGDPNGEYSFMFDEIIKRIDFPIINTDPTGEP
                     LSWRNIPGVSGDTMTAATREKLTSVMKEMDVLNTPLPILYEQNVLGYIHYDTDTRLIR
                     QLAWLPYIEIGLVGAFILIGYIGFSNIKRSEQRSIWVGMAKETAHQLGTPLSSMLGWI
                     ELLREQSEKRESTMKILQEMEVDLRRLDRVVARFSQIGSASDLRRQPVVSILQETAGY
                     FRRRLPQTKQIVIEENYDGDPAIFINAQLLGWVIENLVKNAVDAIEKPQGVIKLSLSK
                     SGDSVYIDVADDGRGIDPDNFKNVFRPGFSTKKRGWGLGLSLAKRIVEDYHRGKIFVK
                     ESRPGLGTTMRIQLNAD"
     gene            complement(6478..7611)
                     /locus_tag="PK937_09385"
     CDS             complement(6478..7611)
                     /locus_tag="PK937_09385"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNH32824.1"
                     /translation="MLKFAKWLPLCIILCSGASGCTGGETREAEEIAPGLQYIRIIQH
                     AVPNTIHILELDWPSTRWTVKAVKAGDHIYDTEPLTKVAASILGTIAAINGDFFSQEG
                     SPSGALVSDGVWIKNGAEIWSSVGFTQSGRVYFEAIRPSAHFKAPGERVTSVQGVNRP
                     RLDNEIILYNAYYGRSTATNNFGTEVRLSVRKIPEAWDRPIEAIVREVDSAQGDHPLD
                     DSTWILSGHGAANMQLLRRMRPGMVVRIDMESGLPHRDLRQLIGAYPVLVREKKSILD
                     GALLNKEFARNANARTAIGYSEARKKLILVCVDGKQANYSVGMNLTELADLMIALGCE
                     SASNLDGGGSTTMFIRDRLVNRPSDSGLERPISDALVIVSADN"
     gene            complement(7687..10215)
                     /gene="dnaE"
                     /locus_tag="PK937_09390"
     CDS             complement(7687..10215)
                     /gene="dnaE"
                     /locus_tag="PK937_09390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNH32825.1"
                     /translation="MGETISEDAQKRLDYELAMIAKMGFPGYFLIVQDFINASRQMGV
                     AVGPGRGSAAGSLVSYAIGITDVDPMRYQLLFERFLNPERVSMPDIDIDFDDRNRGKA
                     IEYVQQKYGTDHVAQIITFGTMAARGVIRDVARALTIPLSEADRIAKMIPKRLDITLE
                     KAVAEVPEMKALETHEDERMRSLINVSRSLEGLIRQPGVHAAGVVITPKPMRELVPIY
                     KSAKDEISTQFDKDWVEKIGLLKMDFLGLATLTILEDAKKLIRQNYNVDVDFDRIPLD
                     DKKTFDLFWRGETTGIFQFESQGMTEYMKQLKPTTIDDLIAMNALYRPGPMEFIPQYI
                     ARKNGKEPVDCFHSNLEPYLKSTYGVIVYQEQVMQVAQELAGFSLGKADVVRRIMSKK
                     KPDELAKIKPEWVDGAVARGYKKELAEKIFEILVPFSNYAFNKSHSAAYAIVAYQTAY
                     LKAHYPGEFMAAVLTSEMANTDRIAVLIHACRDMGVEVLPPDVNESMAEFYVKANNIR
                     FGLGAVKNVGLGAIESILQAREKSGRFKSVFDLAERADLRLVNKKVLESLIQAGACDS
                     LTGHRAQMFLAIEKALSYGQKMQQQANSDQVDIFGQAVQSESRVDIAAIGLPEAPRWT
                     HAEMLQKEKELLGFYASGHPLDRYRAEIEAFSTYHFDDEHELREGEPIRLGGMIAEMR
                     QIFDKKGNPMAFVTLETFGGKMESVVFASVMKECQPMLRKDHVVMLTGKVSIRNEAPS
                     IICDGIYPIGETYLRIRSRVQIHIAQDILQNGILDNLTALLQKAQSANPATSCEVVLC
                     LDFPEKNIIAHYKVSRYKITPTPDILQQIQLLIGQGKIVVKALA"
     gene            complement(10158..11453)
                     /gene="dnaE"
                     /locus_tag="PK937_09395"
     CDS             complement(10158..11453)
                     /gene="dnaE"
                     /locus_tag="PK937_09395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNH32826.1"
                     /translation="MSDFIHLHNHSDYSLLDSCCPVDDLVQAAEQHQMKALALTDYGN
                     LFGAAQFFSAAKAKNIKPIIGMEAYLAPNRLEKNKLIYHIVLFAKDQQGYQNLVALTS
                     QSYMDGFYYKPRIDKALLRAHAAGLIGTSACLKGEIPQRLMHGQYEEAKALALEYLEI
                     FQGDFYLEIQRHGLALDAVVNDGMQRLSRELNIPMIATNDVHYREKADAAAHEILLCI
                     QAGKTITDVTRPKYPGDTFYFRSPAEMKNLFKDVPSAIENTLALAEKISFKMEFGKSQ
                     LPVFGLPEGFTSENEYLAHLANAGLRKKMGETISEDAQKRLDYELAMIAKMGFPGYFL
                     IVQDFINASRQMGVAVGPGRGSAAGSLVSYAIGITDVDPMRYQLLFERFLNPERVSMP
                     DIDIDFDDRNRGKAIEYVQQKWAKRFPKMHKSGWITNWP"
     gene            complement(11563..11639)
                     /locus_tag="PK937_09400"
     tRNA            complement(11563..11639)
                     /locus_tag="PK937_09400"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11603..11605),aa:His,seq:gtg)
     gene            11818..12219
                     /locus_tag="PK937_09405"
     CDS             11818..12219
                     /locus_tag="PK937_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32827.1"
                     /translation="MRPLYIINLILIALVSSCTYGYSASTDVTYSRSYAYKPHNRTVE
                     IYFGDNKPSRAYRQVGLIEVQGGQYYSMDALVAKLRRQAQAIGADAVMDIRKSSTQRE
                     RGSLLDALTDEKPEIYTAPVITGIAIKYIQP"
     gene            12235..12810
                     /locus_tag="PK937_09410"
     CDS             12235..12810
                     /locus_tag="PK937_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32828.1"
                     /translation="MRSLCLIITGYLFMSCVPPQIISNPAPKIAKSKGIIVACEFKTG
                     EVFEFADPGASVVSKGHVVFGRLESGDFTTYEKHQIAYLYTPEKTTWSDVDTTAKVTS
                     IELTDGFRVYFDNEGARIYSAGPCIVGSTLNGSRVVLPLPLVTQTANLTNLKEIKRGN
                     TIGTFFLLAGTVAVLWLYVQFKLQQRESNGY"
     gene            complement(12797..13663)
                     /locus_tag="PK937_09415"
     CDS             complement(12797..13663)
                     /locus_tag="PK937_09415"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNH32829.1"
                     /translation="MRLGIIINTEKESVIAMLPDFLEWLTARHYKFMVNTDAEEFIRA
                     RTSDKTVFADARTLVEASDVLISFGGDGTLLSTAKMVGTAQKPILGVNVGKLGFLTEI
                     ETKELHHAIERLHDGKYTREKRMVLEITLGTRTFYALNDAVIMRGNDGRVIQIGVDVN
                     NAFLNNYIADGLIIATPTGSTAYSLSANGPIMSPTIEAIIINPICPHTLTMRPLVINS
                     EHDVRLTLRSEDRALVSIDGVVEAELSHNDTLIIREAKHFVHLVRFGERNFFDVLRGK
                     LHWGEDDRVSSR"
     gene            complement(13665..14624)
                     /locus_tag="PK937_09420"
     CDS             complement(13665..14624)
                     /locus_tag="PK937_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32830.1"
                     /translation="MKNLFNTKIYALLIIAATTLQAQSDVSVTARTNRDTVAIGENFV
                     YELNIRVPDTSVPIGEPAADFSDAEVVQIRRLEPLTKDGTREEKIQYILSGFKLEPIA
                     IPGPSLRYLIGKDTLFLRANDKTIFIRSELDSAMTDIQPEKPIFYGEVRWWLIALYVL
                     ITIGVLAGAAWLIYRWYKKRQELKNRPKPEPVVIPKKPHEAALEALDALKISGYPERG
                     DMKLWHSEASTIIRIYIEKQFGVPALETTTSELIPSLKRSKIIADADVTILRRMLEVC
                     DLAKFAKYMPSVDDCYELLELAYSFVRHTMPAGEAPPEVKTGV"
     gene            complement(15122..16360)
                     /locus_tag="PK937_09425"
     CDS             complement(15122..16360)
                     /locus_tag="PK937_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32831.1"
                     /translation="MKKYYIFVCFFFFLSMAIQAQMFNNKPYFKLYVELNDSSNQYVY
                     KYLRLSSSNYKKRLLKEIWEQIGARLPKKFVTVDYRAYKTENKYNENITICNDSSLIE
                     EAIWLGFIYNIRLEKDTSESYNRKINLTFDYSLHRTLTEDKDYSGYVSANLDSMSEFD
                     FLQNLSEKITEPVLTNLPQALAIDEAKYGYAILLRINDSYSGGPAVRNKHYSVIRLYS
                     SPSITSWKSKDYKIGDKDLNKNNKSISFGIQYINSIGLSVGVESSVDQILYSKPAGLI
                     KTKSSIHTPDESVASYSIMGSIGYHYLLFSGRLQLSGDLFFGQSNYKTLTQYPYLHIR
                     GYYTSSESFAQGVKLTYIPFSSVRTAIFGSIKWSEEKLELKKIKQKGYSTTDDQIVIE
                     RNPVFNKTQISAGLAFDIKL"
     gene            complement(16379..17401)
                     /locus_tag="PK937_09430"
     CDS             complement(16379..17401)
                     /locus_tag="PK937_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32832.1"
                     /translation="MEERELLVFAYEYVYQDMPGEAFVAIPFSDSTIAVAGYTDSAGS
                     SIAFFARFSIDGEMKWRVDPLNGKKGSILGGIKSSDGTVILTGEIEGSTGDKDIVVLK
                     TTYEGEVLWLKTWDSTLADKGLAVKEAEDGKIHVLGLAQNFKLVILILDGQGNEIHVN
                     WTELVGHNNCRSIIPRNGGAGYVVAESGEWMSTFSYNGTGQINLLRQSRRGAKASSAA
                     DIGGGLFAYVGNSSNGSFLSYVNPIAFSEATVIVDDIHSVLNSVYSNKDGSIDVVGNN
                     GDELFVKSYDKNRMLLKSFFYGKSEIVFSARVMTSITGNEKIFVGSATTTNTLNKYVL
                     ILKSLE"
     gene            complement(17851..18621)
                     /locus_tag="PK937_09435"
     CDS             complement(17851..18621)
                     /locus_tag="PK937_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNH32833.1"
                     /translation="MCLVVWALAAHPQYRLIVAANRDEFYARPTEVSKWWADHNNLLA
                     GRDLQGGGTWMGVTRGGRFAVITNFRDPSSEKKRATTRGRLVEQFLRSEEAPDIYVQA
                     VVSQGESYNGFNLIVADPSRMAYVTNTQPGWSWVEPGIYGLSNHRLDTPWPKVARTKK
                     RFSEILRSDEPDTNAMMEMMSDRETAPDKDLPVTGVGIEWERKLSPVFIQMPHYGTRT
                     TTIFMEDHTGRCTWIERTWDPIRAQFKLKKDTWTKMVV"
     gene            complement(18624..19157)
                     /locus_tag="PK937_09440"
     CDS             complement(18624..19157)
                     /locus_tag="PK937_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH32834.1"
                     /translation="MIVSLSSKWSRIEASKTYFVTCVQAMNEHQSHWRPKLDAWHALD
                     VLQHLVLVEEGMAKQLHHREAILHKKTVLPKIIGLPAYWLTFRMGMRVKAPVKSVLPQ
                     EVMTLAMCVQRWDAVRDVMKNFLETITSETEGAIYFYHPITGQRTCEQMLDFLYDHLR
                     HHQAQMRRIMHHPKYPA"
BASE COUNT         4913 a         4782 c         4345 g         5278 t
ORIGIN      
        1 acctctttga tcaaatttaa agaacaatcc cagcgacgat cagtcataac tatttagtat
       61 atgaattgcg tttatagggt gcgcagtcga gtaatatgcg tattgcgtaa aaggcgccat
      121 tttcgggtga tatgcgcaat agcgttgagg atggtgggat caccctcccg aacgataccc
      181 gtcttagggt ggtcggtgta ccaacgatcc aaagggctct ctattttaga aagctcgcgg
      241 ctgcttacat gggtataaat ttcggtggtt ttggaactct cgtggccaag caactcctgt
      301 atataacgta tatccacccc cgattctaaa agatgcgtag caaaactatg gcgtaacgaa
      361 tgcacagata ctcttttttg aataccggca cgggaagccg cgtttgtaaa catttgctgg
      421 atcgaacgca cactataccg accaccatgt acaccttcaa agaggtattt tttgggccga
      481 tactgtttgt aataatctct taaaagcttc aaggcctgat cggataaaat agtaaaccga
      541 tcctttccac ctttaccatt acgaatatga agcagcatac gtctggaatc aatgtcgtta
      601 acgttcaggt tcaccgcttc accgacacgt aaaccggatg aatagatcaa aaaaagaata
      661 cagcggtgtt tcatgttttt gacggaagca aaaataccgc gcaactcatc ctgactcaga
      721 acaaccggca aacgcactgt aacgcgtgca cgctgatatg ccaaattcat tttttcgcga
      781 ccgagaaaat tttcaaagta acatttgaat gcgttgatcg cctgattttg atatgacggc
      841 gaaacacccg ccggaaccag cacatcttgt atatacataa gcatatcgtg ttccgtgaga
      901 gacgacggca accgatcttt gtaatgcgtg aaaagacgct tgagctgcat ttcgtaagca
      961 cccaaagtat tggaactata ccggcgcagc ttgagcattt cgatgaaagc tttcatgtgg
     1021 ccacccagag aatttattga agaactgatc aatccctgac gaaacttagc acaacagtgt
     1081 tttgaggtca agtaaaaaaa tgaaaatcgc gacctacgtc agagataaaa taaaaaggct
     1141 tcatcacata ttgatgaagc cttttgtatt caaatcaaat acaaagctaa ctatggcgct
     1201 gccggagccg attcgtcagg aattttagtt aatttatctt tttctttacc gacaagatct
     1261 ttgagtttgg cgatcttttc ttcagcggct ttaagttttt cttccttttc tttgagttta
     1321 tcgcctttga gttgtccgga tttttttttc ttttcgacat tttcttttgc cgttttaata
     1381 cgcgcttcgg tcgcggcaat tttctcttcg gcaccggtta ttttttcatg cgcatttttg
     1441 cgcttttctt tcattttcaa tttcgcatcg gctgaacgca ttttcccgaa ttccttacca
     1501 ctcatttcgc ctttattttt cccgtacgcg ttgccttcgg accccttgcc ttcaggtttt
     1561 cctttgggct gtagttcgtc ttcgctttta acttccttct ttgctttttc agttttgccg
     1621 cctttttctt ctttatgcgc tttacctttt tcggcgttgg gattttgggc catcgtacta
     1681 cccgcgctta acgcgagtgc gagtacggcc aatacaaata attttgcagt ggttttcata
     1741 tttttttcct cacgttgcgg ttaaatgtct ttgagtaaat cgtctacggc tttttccgtt
     1801 tcatctacct gttgttgcag gctttcggtc gaagcgttga gatcgtttgc gacggaatca
     1861 atccttacgg cttctttgtg atccaattcc tcagcggatg gttttttttc accgcatgcc
     1921 gcaatgcccc acaaggcgag caccgcaagc atcatgatgt actttctcat accttactcc
     1981 tcatttggtt agttttggag agtcaatcca agtagctcaa atcatattac gcgttgatat
     2041 tgatctaacg tcatcgtcca aactctttcg ccacaaaaac ggtcgccctc ggggcaatac
     2101 ggacgcatgc ccgcgcgaag tcgtataccg cacggcggta aaagaagatt tccaatcacg
     2161 ggattgactt gctggatttg ttgcgtttcg tccaccgatg cgcgccagat ttcttcctgc
     2221 gccagataac acaagcgcat tgccattttg tgatgcaggt tttgcagatc tgcggattcc
     2281 gtaaaacgta tggctaccgc attgggcaaa agatacatcg cgttttcgtc ggacacatga
     2341 tgtgcacgca gacgcgcgat atattcccat gtccgtgtca tgacctcgtc ataatacgcg
     2401 tggacgtttg cgtcttccaa aatcaacgcc ggcgtaatgt aatccggctc ggccagacga
     2461 tgtgccgtga gaataggacg tgagcccggc gtcatacgat gccgctgatc ttgcgagtcg
     2521 gcggcatggc ttattttttt gcgaaacgta tagtgcgcgt gcaccaacgc ccgcgttatt
     2581 ttggacatcg tcgtcaagtt gagcgattcg ccgtacaatt tatttttcga agggtcgagc
     2641 gccagcgcga tcgcatccgc atcggagaga cgctcacggg tgagacccaa tacttcccgc
     2701 acgctttggg caacaacagc ttcgttattg attttgtaat ccaccagctt tgacatgcgg
     2761 ccatcaaggc tacgatcaaa ctcggcagca aacgctttgc ggccggcgtc atcgtaaaaa
     2821 tccggatgcg cctgataaaa taaatattcc ggggtttctt cgatgggcaa agcttcttca
     2881 agaatcgctt tgtacatcgg gtccaaacgt atcatctcct gcaccatctg ctcgacgacg
     2941 acgcgctgtt cgacaggcgt gtccatctga ttgcacaagc gataataccg caacagcgtg
     3001 ataccgctta ccgtgtgata caaatacgaa aacgccgcca ccggtaaaac gtatcgcgaa
     3061 atttcctggc attttttttg aatttccttc gcatactttt cgcggacttt ggctctggcg
     3121 ggaaaccgtt tgaaataggc ggcctccgcc gacgggcgca gcatctcatt gagtttaaaa
     3181 tacgcggcgg tttgaaaggc aatcgcttca cggtaaatcg ccagcgcctc gccctccagc
     3241 gcaggtatca aaaacgtatc gggcttgacc tccacataac gctggctgac ctgttctgaa
     3301 ttataaaacg ggtggctgtg taaaaacgtc cacaaaaact gacgggagac attggccaat
     3361 tcgaattgga aatacgcatg cgagaagacc gtgtgatggc cggcctgata cacgctttgt
     3421 gcgagcggta cgtgtttatc ggtgatctgc tcgtcgcgaa caataccttt ggccgaataa
     3481 cacgtgcgcg ccgtagcgat cgcattttga aacggcgtcg aaaacgattt tgaaagtact
     3541 acgtgcggtt tgggggaaag aaatgtatgc aatgtattag aagccctgtt ttgattattt
     3601 cacttcgatg tcgcgcccgt tcacttaacg attgctccag ttcggtttac gtttttcgct
     3661 gaatgctttt aacgcttcga ggcgatcctc ggtcggtatc acatcgttgt agcattcgtt
     3721 ttcgatctcc agcgcatttt gcaggctcgt acccataccc agacgcacgg cttttttggc
     3781 ttgtcgtaca gcgataggcg cattggccag tatctcctct gccaaagctt gcgcacgcgc
     3841 atgcagttcg ttcaatgtac aaacttccat cgccacaccg tcgtgcaggg cttcctgcgc
     3901 cgtaaacacg cgtgcggtga gtatccagtg caaggctttc tgatatccga taagtcgcgg
     3961 caaacgctgc gtaccgccgg cgccgggaat aataccgagc gccgtttcgc gcaaacccgt
     4021 tttaacgtcc tcggaaaaaa ttcgaaagtc gcacgccaaa gccaactcac atcctccgcc
     4081 cagcgcactg ccgttgacgg ccgcgatggt cggtacttcg aggtcgccga ttttgtgaat
     4141 ggtaccgccg ataatgtaac ggacaaattt tttgacatcg gattcgctca tgccctgccg
     4201 ttctttcaaa tcggcaccgg cgcaaaaatt atccaattcc gaacgtagaa tcacgactcg
     4261 cgccgtgtca tcctgacgaa tacgatcaca ggcttcggat aactccccga gcaattcgcg
     4321 attgagcgca ttgactttgg ggcggcgcaa tccgatggta atgataccgt tatcgttggt
     4381 gtagtttata ttttgcatac aagctgattc tcttggtcat tcattgttag aattttattt
     4441 ttttaaattc gtcgtaagcc catcatctat gttcttttct ttggcttgat ccgaagaaaa
     4501 gaatcaaaaa aaaatcacgg ctgcagagaa cccgcctttt caaaacgatt cctattcgta
     4561 tcttgtttac tttgaacaaa ccattaaatc cggtgcatgg ctttgaaaac gtcttcatgc
     4621 tgcgccagaa tacgaatacc caatgtttcg ctggtacgtg ccagattttc tttgatggta
     4681 ccaaaggggc atgcggcgtt tttgatatcc acgagcatca tcagcgtaaa aaattcctgc
     4741 attatttttt ggctgacatc caaaatgtca caacggcaat cggtgagcgt ctgcgaaata
     4801 ttggaaataa taccggggcc gtttttgccg aatgccgtca caatgatgcg gccttcggtg
     4861 cgattgaggt aacgaaacgt gctgtgatgt tcctgcacgt accctttgtc accgtcaagc
     4921 cggcgaatcg cttccatggc aacgtgtttt acggtttcat ccgatgcttg tgcaccgagc
     4981 tggcggcgcg cttcttcgac gatcttgcgg atttcctgtt cgttcatcgt ggtttatact
     5041 gttatggtta aaaaaaatgg tttccggtta atcggcgttg agctgaatac gcatcgtcgt
     5101 acccaaaccc gggcggcttt ctttgacaaa tattttgccg cgatggtaat cttcgacaat
     5161 ccgtttggcc aaactcaagc cgaggcccca gccgcgtttt ttggtactga agccgggacg
     5221 aaatacattt ttgaaattat cgggatcaat gccgcgtcca tcatcggcga cgtcaatgta
     5281 cacagaatcc cccgatttac tcaaggacaa tttgataacg ccctggggtt tttcgatcgc
     5341 gtccacggcg tttttgacta agttttcgat aacccatccg agcaattgag cgttgataaa
     5401 aatggccgga tcaccgtcgt aattttcttc gatgacgatc tgtttggtct gcgggagacg
     5461 gcggcgaaaa tacccggcgg tttcctgcaa aatcgaaacg accggctgac gacgtaaatc
     5521 cgacgccgaa ccgatctgcg aaaaacgcgc cacgacacga tccaatcgcc gcaggtccac
     5581 ttccatttcc tgcaaaatct tcatcgtgct ttcgcgtttt tcagattgct cgcgcaataa
     5641 ttcgatccag cccaacatgg aggacagcgg cgtacccaac tgatgcgctg tttctttggc
     5701 catacccacc cagatggaac gctgctcgct gcgcttgata ttgctaaatc cgatgtatcc
     5761 gatcaggata aacgcaccga caaggccgat ctcaatatat ggaagccacg ccagttggcg
     5821 gatcaaccgc gtatccgtat cgtagtgaat ataaccgagt acgttttgtt cgtacaaaat
     5881 cggcagcggc gtattcagca catccatttc tttcatcacc gatgtcagtt tttcgcgcgt
     5941 ggccgccgtc atcgtatctc cggaaacgcc gggaatattg cgccaggaaa gcggttcgcc
     6001 cgtgggatcg gtattgatga tgggaaaatc aatgcgtttg atgatttcat caaacataaa
     6061 actgtattcc ccgttgggat caccttcgcc gatatgtgcg ataagatggg tgtaaatgcg
     6121 tacaaggtga cgcgaatcgc cgcggagttt ttcgacgaca tactgcgtat ataatacgac
     6181 gccgaatatc atcagtatcg ctacggcgaa aaggccgctt ttgatattga tgttgagacg
     6241 gtatgggtcg tacaccgtgc gtgtattgcg ggatgaatcg gatgtttctt ttttcacatc
     6301 ggttgggagt taattttacc cgtcagttta tgtatgaatc cgcgatcatt tcggttcggc
     6361 aagcctgcgg aatacctgct cgctaaggcg ctaaggcaca aagtaatata ctaaaaaatt
     6421 ccatttcgct ttgcggcttt gcgaggatat gttttttatc ggtcacttcg ttgcgcatta
     6481 attatcggca cttacaatga cgagcgcatc ggaaatcggg cgttctaatc cgctgtccga
     6541 aggacgattg accaaacgat cgcgaatgaa catggtcgta gaaccgccgc catcaagatt
     6601 ggaagccgat tcgcaaccga gtgcgatcat caagtcggcc agttccgtca ggttcatgcc
     6661 cacgctgtaa ttagcttgct tgccgtccac gcataccaaa atcagttttt ttcttgcttc
     6721 ggaatagccg atcgccgtgc gggcattggc attgcgtgca aattctttat tgagtaatgc
     6781 gccgtcgaga atacttttct tttcccgtac gagtaccggg taagcgccga tcaattggcg
     6841 caagtctcta tgtggcaggc cgctttccat atctatacgc acgaccatac ccgggcgcat
     6901 gcgacgcagc aactgcatat tggccgcgcc atgcccgctc aaaatccagg tggaatcgtc
     6961 cagcggatga tcgccttggg ccgaatccac ttcgcgtacg atcgcttcga tcggccgatc
     7021 ccatgcttcg ggaatttttc tgacgctcag ccggacttcc gtgccgaaat tattggtcgc
     7081 cgtcgaacgg ccataatacg cattgtacaa aataatttca ttgtccaggc ggggacggtt
     7141 aacaccttgc acggaagtta cacgttcacc cggggctttg aaatgcgcac tcggacgaat
     7201 tgcttcaaaa tacacgcgac cgctttgtgt gaatccgacc gacgaccata tctccgcacc
     7261 gtttttgatc cagacgccgt cgcttaccaa cgcgccggaa gggcttcctt cctgagaaaa
     7321 aaaatctcca ttgatcgcgg cgatagttcc gagtatggaa gccgccactt tggtaagggg
     7381 ttcggtgtca tagatatgat cgccggcttt gacggctttg accgtccatc gcgtggaagg
     7441 ccaatctaac tcgagaatgt ggatcgtatt gggtacggca tgctggatga tgcggatgta
     7501 ttgcaatccc ggggcgattt cttccgcttc gcgggtttcc ccgcccgtac aaccgctcgc
     7561 ccccgaacaa agtataatac aaagcggcaa ccatttggca aatttcaaca tggcgctaac
     7621 atcgttaatc gggagatttc ttgcaagcgg aatatcatgc gctcggcagg aaaaggcggc
     7681 atccgatcaa gccagcgctt tgactacgat ttttccctga ccgatgagta actggatctg
     7741 ctggagtatg tccggcgtcg gtgtgatttt gtaacgggac actttgtagt gggcgatgat
     7801 gtttttttcg ggaaaatcca gacagaggac gacttcgcag gacgttgccg gattagccga
     7861 ttgggctttt tgtaaaagcg cggtgaggtt atccaaaata ccgttttgga gaatatcctg
     7921 tgcgatgtgg atctgcacgc gggagcggat gcgcagatag gtttccccga tgggatatat
     7981 cccatcgcag ataatgctcg gcgcttcgtt tcgtatcgag actttaccgg tcagcatgac
     8041 cacgtgatct ttgcgcagca tgggctggca ttctttcatc acgctggcaa agaccaccga
     8101 ttccattttt ccgccgaatg tttcgagcgt cacaaacgcc atcggattgc cttttttatc
     8161 aaagatctga cgcatctcgg cgatcatacc gccgaggcga ataggttcgc cttcgcgcag
     8221 ttcgtgttca tcatcaaagt ggtaggtgct aaaggcttcg atttctgcgc ggtagcgatc
     8281 cagcggatga cccgacgcat aaaatcccag cagttctttt tctttttgaa gcatctcggc
     8341 atgcgtccag cgcggcgctt caggcaaacc gatcgcggcg atatccacac gcgattccga
     8401 ctggacggcc tgaccgaaaa tatccacttg atcggaattg gcctgctgtt gcattttttg
     8461 gccgtagctc aacgcttttt cgatagccaa aaacatctgt gcgcggtggc ccgtgagcga
     8521 atcgcaggca cccgcctgaa tcagactttc gagaactttt ttattgacca atcgcagatc
     8581 ggcgcgctct gccaaatcga aaacggattt gaaacggccc gatttttcac gtgcctgcag
     8641 gatggattcg atcgcaccga gacccacatt tttgaccgca cccagaccaa aacggatatt
     8701 attggctttg acataaaact ccgccatgga ttcattgaca tccggcggga gcacctccac
     8761 gcccatatcg cggcacgcat ggatgagaac ggcgatgcgg tcggtattgg ccatttcact
     8821 ggtaagtacc gccgccataa attcgcccgg gtaatgagct ttgaggtacg ccgtctggta
     8881 cgctacgatc gcgtacgctg ccgaatggga tttgttgaat gcgtaattgg aaaacggaac
     8941 gaggatttca aatatttttt cagccaattc ttttttgtaa ccgcgggcga cggcgccatc
     9001 cacccattcg ggtttgatct tggccagttc atccggtttt ttcttggaca tgatacgccg
     9061 cacgacatcc gctttgccaa gcgaaaaacc ggccaactcc tgcgcgactt gcatcacctg
     9121 ctcctggtac acgataaccc cgtaggtgga tttgagatac ggctcgagat tggaatgaaa
     9181 acagtccacc ggttctttgc cgtttttccg ggcgatatac tgcgggatga attccatcgg
     9241 ccccggacga tacaaagcgt tcatcgcgat cagatcgtct atcgtcgtgg gtttgagttg
     9301 cttcatgtat tcggtcatac cctgggattc aaactgaaaa atcccggtgg tttcaccgcg
     9361 ccaaaacaga tcgaacgtct ttttgtcgtc cagcggtatg cggtcaaaat caacatcgac
     9421 attgtaattt tggcgaatta gttttttggc atcttccaaa atcgtgagcg tcgccaaccc
     9481 gaggaagtcc attttgagca gcccgatttt ttccacccag tctttgtcaa actgggtcga
     9541 aatttcgtcc ttggctgatt tgtagatcgg caccaattcg cgcatgggtt tgggtgtgat
     9601 cacgacaccg gccgcatgaa caccgggctg gcgaatcaat ccttccagcg accgcgagac
     9661 attgatcagc gaacgcatac gttcgtcttc atgcgtttcc agcgctttca tttcgggtac
     9721 ttcggcgacc gctttttcga gcgtgatgtc cagccgtttg ggaatcattt tggcaatgcg
     9781 atcggcttcg ctgagcggta tcgtgagcgc ccgcgctacg tcgcggatga cgccgcgtgc
     9841 ggccatcgta ccaaacgtga tgatctgcgc cacatgatca gtgccgtatt tttgctgcac
     9901 gtattcgatg gctttgcccc gattgcgatc atcaaaatca atatcaatat cgggcatgga
     9961 aacacgttcg ggattgagga accgctcgaa cagcaactga tagcgcatgg gatccacgtc
    10021 ggtgataccg atggcgtacg acacgaggct gcccgcagcc gaaccgcgtc ccggacccac
    10081 cgccacaccc atctgacgcg acgcattgat aaaatcctgc acgatgagaa aatatcccgg
    10141 gaaacccatt ttggcgatca tggccaattc gtaatccaac cgcttttgtg catcttcgga
    10201 aatcgtttcg cccatttttg ctgcacgtat tcgatggctt tgccccgatt gcgatcatca
    10261 aaatcaatat caatatcggg catggaaaca cgttcgggat tgaggaaccg ctcgaacagc
    10321 aactgatagc gcatgggatc cacgtcggtg ataccgatgg cgtacgacac gaggctgccc
    10381 gcagccgaac cgcgtcccgg acccaccgcc acacccatct gacgcgacgc attgataaaa
    10441 tcctgcacga tgagaaaata tcccgggaaa cccattttgg cgatcatggc caattcgtaa
    10501 tccaaccgct tttgtgcatc ttcggaaatc gtttcgccca tttttttgcg aagcccggcg
    10561 ttggccaaat gcgcgaggta ctcattttcc gacgtaaatc cttccggcaa tccgaaaacg
    10621 ggcaactggg atttgccaaa ttccatttta aaggaaattt tttccgccag ggcgagcgtg
    10681 ttttcgatcg ccgaaggaac atccttaaag agatttttca tctctgcggg cgaacgaaaa
    10741 taaaacgtat caccgggata cttgggacgc gtcacatcgg tgatggtttt acccgcctga
    10801 atacaaagta gtatttcatg cgccgcggca tccgcttttt cacggtaatg cacatcgttg
    10861 gtagcgatca tcggaatatt cagttctcgg gataaccgct gcatgccgtc attgaccacc
    10921 gcatcgagcg ccagtccgtg ccgctgaatt tcgagataaa aatcgccttg aaatatctcc
    10981 agatattcca gcgccaacgc tttggcctcc tcgtattgcc cgtgcatcag acgctgggga
    11041 atttctcctt tgaggcatgc cgatgtaccg atcaatcccg cggcgtgcgc gcgaagcagg
    11101 gctttatcaa tacgcggttt ataataaaaa ccgtccatat aactctgcga cgtcagcgcg
    11161 actaaatttt gataaccctg ctgatctttg gcaaaaagta caatgtgata gatgagctta
    11221 tttttttcta atcggttagg cgcgaggtaa gcttccatac cgatgatggg cttgatgttt
    11281 ttagctttgg ccgcgctaaa aaattgggcc gcgccaaaaa gattgccgta atcggtcagc
    11341 gccagcgctt tcatctgatg ctgctcggcg gcttgtacaa gatcatccac cggacaacaa
    11401 ctgtccagaa gactgtaatc ggaatgatta tgaagatgta tgaagtcgga catcgggtca
    11461 ggattaattt attgggaaga caccgtaaga caaaataaac aatcccgcca aaaaacaaaa
    11521 tgcgaagttg attcaactcc gcattggatg ggatcttttc gatggggcga gcgacggggc
    11581 tcgaacccgc ggccttcagg gccacaacct gacgctctaa cctgctgagc tacgcccgcc
    11641 atgaaacgtg ccgaagatag tgcaccgcat ttatatttgc aaggggaaat tgggttttat
    11701 cacagtgtat tttaatttca catccgactc atcccaacac ttcaaataat ttcaacacgt
    11761 taaaaaacgg cactgacttt gcattatcct ccggcgttca cttaaaccgg agaatttatg
    11821 cgcccccttt acatcatcaa cctgatttta atcgcgctcg tcagcagttg cacgtacggc
    11881 tactcggctt caacggatgt gacctattcc cgctcctacg cctacaaacc gcataaccgc
    11941 accgttgaaa tttatttcgg cgataataaa ccttcccgtg cgtaccggca ggtcggattg
    12001 atcgaagtgc aaggcggcca atattacagt atggatgcgt tggttgccaa attacgaaga
    12061 caggcgcagg ctatcggcgc cgatgcggtt atggatattc gcaaatcatc cacccagcgc
    12121 gaacgcggca gtttgctgga tgccctgacg gatgaaaagc ctgaaatcta taccgcgccc
    12181 gtgatcacgg gtatcgcgat caaatatatt caaccttaat tcgcggagtt ttttatgcgt
    12241 agcttatgcc tcatcatcac cggttatttg tttatgtcat gcgtaccccc gcaaatcatt
    12301 tccaatcccg ctcccaaaat cgcaaagagc aaaggcatca tagtcgcttg tgaatttaaa
    12361 accggcgagg tatttgagtt cgccgacccc ggcgcatccg tcgtttccaa agggcacgtc
    12421 gtatttggcc gattggaatc cggagacttt acgacttacg aaaaacatca aatcgcttat
    12481 ctctatacac ctgaaaaaac cacatggtcc gacgtggata ctacggccaa agtcacttct
    12541 atcgaattaa ccgacggatt tcgcgtatat tttgataacg aaggcgcacg gatctacagc
    12601 gccggccctt gtatcgtcgg tagtacgctc aacggaagtc gtgtcgtgct gccgcttccg
    12661 ctcgtaacgc aaacggctaa ccttaccaat cttaaagaaa taaaacgcgg gaatacgata
    12721 ggaacttttt ttctactggc gggaaccgta gccgtattgt ggttatacgt gcaattcaaa
    12781 cttcaacaaa gagaatctaa cggctactga cgcggtcatc ttcaccccaa tgcagtttgc
    12841 cgcgcaacac atcaaaaaaa ttgcgctcgc cgaaacgcac aagatgaacg aagtgtttgg
    12901 cttcacgaat gatcaacgta tcgttgtgcg aaagttccgc ttcgacgaca ccgtctattg
    12961 agacgagcgc gcggtcttcg gaacgcaacg tcaatcgcac gtcgtgttcg gaattgatca
    13021 ccaaaggccg catggtgagc gtgtgcgggc agatcggatt gatgatgatc gcttcgatcg
    13081 tcgggctcat gatgggcccg ttggctgaaa gcgaatacgc ggtcgaaccc gtcggcgtcg
    13141 cgatgatgag tccatcggca atataattat tgagaaacgc attattgaca tccacaccga
    13201 tttggatcac gcggccgtca ttgccacgca tgatcacggc atcattgagc gcataaaatg
    13261 tgcgtgtacc gagcgtgatc tccagtacca tacgtttttc acgcgtatat ttgccgtcgt
    13321 gcaggcgctc aatcgcatga tgcaactctt tcgtttcgat ttccgtcaaa aaacccaatt
    13381 tgccgacatt gacgccgagg atcggttttt gtgcagtacc gaccattttg gccgtggaga
    13441 gcaatgtgcc gtcgccccca aaggatatca ggacatccga cgcttcgacg agcgtgcgcg
    13501 catcggcaaa caccgttttg tcggaggtac gggcacggat aaattcttcc gcatcggtat
    13561 tgaccatgaa cttgtaatgc cgcgcggtga gccactccag aaaatccgga agcatggcga
    13621 tcacactttc tttttccgta ttgatgataa ttcccagacg catgtcaaac tcccgtttta
    13681 acttcaggcg gcgcttcgcc ggccggcatc gtgtggcgca caaacgaata agcgagctcc
    13741 aacaattcat aacaatcatc taccgacggc atgtacttgg caaacttggc cagatcgcac
    13801 acttccagca tgcgacgcaa tattgtcaca tcagcatctg cgataatttt ggatcgtttt
    13861 aaagacggta tgagttcgga tgtcgtcgtc tccagcgcgg gtacgccaaa ttgtttttcg
    13921 atatagatac gaatgatggt agatgcttcg ctatgccaga gtttcatatc cccgcgttcc
    13981 ggataaccgc tgattttcaa agcatccagc gcctcgaggg ccgcttcgtg cggttttttg
    14041 ggaatcacaa ccggttcggg tttcggtctg ttttttaatt cctgcctctt tttgtaccaa
    14101 cggtaaatca accacgccgc gcccgccaat acaccgatgg ttatcaggac atacagcgcg
    14161 atcaaccacc aacgcacttc gccgtaaaaa atcggcttct ccggttggat atcggtcatc
    14221 gcactgtcta attccgaacg aataaaaatc gtcttatcgt tcgcccgcag aaaaagcgta
    14281 tctttaccga tcagataacg cagcgacggc ccgggaatcg caatcggctc gagtttgaaa
    14341 ccggataaga tgtactggat tttttcttcc cgtgtaccgt ctttggttag gggttccaaa
    14401 cgccgaatct gtaccacttc cgcatcggaa aaatcagccg ccggttcgcc aatgggtacg
    14461 gatgtatccg gcacgcggat attgagctca tacacaaaat tttcaccgat cgcgacagta
    14521 tcccgatttg tacgcgccgt caccgacacg tccgactggg cttgcaacgt cgtagccgcg
    14581 atgatcaaaa gcgcgtatat tttggtatta aataaatttt tcataaacgc ggctaatgta
    14641 taaaaattga taaccaatgg caatgcaagg ctttaagttt ggatatattc ggatcggcta
    14701 caaaaaatgc agttcgttta taaatccgtt cgatgccatg cgtgtcggtg caacgtaaat
    14761 cgttctaaaa cgggatccca cgtgcgctct atccatgtac atcgcccggt atgatcttcc
    14821 ataaaaatcg tcgtggtgcg cgtaccgtag tgaggcaata tatatcggag aggttgtata
    14881 ttatgattta gaaaaaacag ccgaaactaa ttgcgtcatc tatcgaaata cataatcttc
    14941 aaaccaaact aagtcatcag aatacaggcc ggtacagatc atcttaggta atcaggctta
    15001 aatctgtctt actgacggca atatctttat aatgatgaca cacaaccttc ccatatttct
    15061 ttcagaaaaa tctaactttg attccaaaat ctaacgcaac actttttgtg tgaatttatg
    15121 tctacaactt gatgtcgaat gccaatcccg cactgatttg cgtcttattg aaaacaggat
    15181 tacgttctat tacgatttga tcgtccgtcg tactatatcc tttttgcttt atttttttca
    15241 actctagttt ttcttcgctc cattttatac tcccaaaaat tgccgtacgc accgacgaaa
    15301 acgggatata tgtcagtttc acgccttgtg cgaaactttc tgaggaagta taatatcctc
    15361 ggatatgtaa gtaaggatac tgcgttaagg ttttataatt cgattgacca aaaaacaaat
    15421 ctcccgataa ttgcaaccgc cctgaaaaca acaaataatg atacccaata cttcccatga
    15481 tcgaataact tgcaacggat tcatctggtg tatggatact gcttttcgtc ttaatcaaac
    15541 ccgccggttt tgagtataaa atctgatcaa ctgaagattc aacgcctaca gacaatccaa
    15601 tcgagtttat gtactggata ccaaaagata tagatttgtt gtttttattc aaatctttat
    15661 ctccgatttt gtaatccttc gatttccaac ttgttatgga cgggctagag tataatcgaa
    15721 tcacagagta gtgcttgttt cttacagcgg gtccaccgga ataactatca ttaatccgta
    15781 gtaaaatggc gtatccatac tttgcttcgt caattgctaa tgcctgagga aggttagtta
    15841 aaacaggctc ggtgattttt tccgataaat tttggagaaa atcaaattca gacatagaat
    15901 ccaaattagc tgaaacgtac cctgagtagt ctttatcttc tgtaagcgtc ctgtgcaatg
    15961 aataatcaaa cgtcaaattg atttttctgt tgtaactttc agatgtgtcc ttttctaatc
    16021 ggatgttata tataaagccc aaccatatgg cttcttcaat caaagatgaa tcattacaaa
    16081 ttgttatatt ttcattatat ttattttcgg ttttgtaagc tctgtaatca acagtcacga
    16141 atttttttgg caatcgagcg ccaatttgtt cccagatttc ctttagcaac ctttttttat
    16201 agtttgagga agataacctt aaatacttat aaacgtattg atttgacgaa tcatttaact
    16261 caacatacaa tttaaaataa ggcttattgt taaacatttg cgcttggatt gccatcgaaa
    16321 gaaagaaaaa aaagcaaacg aaaatataat attttttcat aaaactctaa ttccttaatt
    16381 actcaagtga ttttaaaatg agtacgtatt tgttaagtgt atttgtcgtc gtagcgctac
    16441 cgacaaatat tttttcattc ccggtgatgg atgtcatgac cctggccgaa aagacaatct
    16501 cactttttcc ataaaaaaac gatttcagca gcattctatt tttatcatag cttttcacaa
    16561 atagttcgtc accattattt ccaacaacat ctatggagcc atctttatta ctataaactg
    16621 aattcaatac agaatggata tcatctacta taacggttgc ttcactgaaa gcaatcgggt
    16681 ttacgtatga tagaaaacta ccgtttgagc tattcccaac ataggcaaac aatcctcctc
    16741 ctatatccgc ggcagatgaa gctttagcgc ctcgccttga ttggcgtaat aaatttattt
    16801 ggcctgtacc gttatatgaa aaagttgaca tccattcgcc ggactcagcc acgacatatc
    16861 cggcgccacc gttacgggga attatagaac gacaattatt atgccccacc aactcagtcc
    16921 agttgacatg tatttcattg ccctgaccat caaggattag gatcaccagt ttgaagtttt
    16981 gggctaaacc taaaacatgg attttcccgt cttcggcctc ttttacagcc aagcctttat
    17041 ccgctagagt tgaatcccat gtttttagcc aaagaacttc cccctcatag gttgttttta
    17101 atacaacgat atctttgtct ccagttgaac cctcgatttc accggtaaga atgaccgttc
    17161 catccgaaga ttttatcccc ccaagaatag agcctttttt tccgttaagt ggatccactc
    17221 tccatttcat ttcgccgtca atcgaaaaac gcgcaaaaaa cgctattgac gaaccggcgc
    17281 tatccgtgta gcctgcaacg gcaatagtac tgtctgagaa tggaatcgca acaaaagcct
    17341 ctcccggcat atcttgataa acgtattcgt aagcaaacac taataattcc ctttcttcca
    17401 aagaacaacc aatgaacgct agtaacatga aaaaaaaatg gagaggattg aagagttttt
    17461 ttcttattgg gttcatacat tacactatta atttgttatt ttcttattcg tgaattaaaa
    17521 aacattattc tgaattgaga aaggaaagca tgtagtgatt gcccccgacg tacaaataca
    17581 aattttacag atgctgacaa attcagccaa gaacacatag tttagtaccg gtaaattttt
    17641 taaactctag atggattttt tggattaaat ttaaacagac tctactaaaa gatagaaaac
    17701 tttacgagcc cactaataac aacaatatat ttattacaaa aacaagccgt atatgctaat
    17761 aaaaattctc tgaaagtcaa tttcaaaaaa atcatttttt gacgagtcat cacaaaaaac
    17821 tgtcctcgtc tatagtacct ttttactcga tcaaaccacc attttagtcc acgtatcctt
    17881 tttcaattta aattgagcgc gtatgggatc ccacgtgcgc tctatccatg tacatcgccc
    17941 ggtatgatct tccataaaaa tcgtcgtggt gcgcgtaccg tagtgaggca tctgaataaa
    18001 taccggtgac agtttgcgct cccactcaat acctaccccg gtcaccggca gatctttgtc
    18061 cggcgcagtt tcacgatcgg acatcatctc catcatcgca ttcgtatcgg gttcatcgct
    18121 acgcagaatt tcagaaaacc gttttttggt gcgggcgact ttgggccacg gtgtatccag
    18181 acgatgatta ctgagaccgt agatacccgg ttcaacccat gaccaaccgg gttgcgtatt
    18241 ggtgacatac gccatacggg acggatcggc gacgatgaga ttgaagccgt tgtaggactc
    18301 gccttgggaa actacggcct gcacatatat atccggcgct tcctcggagc gaagaaattg
    18361 ttcgactaat cgcccgcgtg tcgtagcgcg ttttttttca gaactcggat cccgaaaatt
    18421 ggtaatcacg gcaaatcgtc cgccgcgcgt cacacccatc cacgtaccgc cgccttgcaa
    18481 atcacgtccc gcgagtaggt tattatgatc cgcccaccac ttcgaaactt ccgtcgggcg
    18541 cgcgtaaaat tcgtccctgt tggcggcgac gatcaggcgg tattggggat gggctgctaa
    18601 agcccagaca acaaggcaca tggtcaggcc ggatatttgg ggtggtgcat aatacgacgc
    18661 atctgcgcct gatgatgccg gagatgatcg tataagaaat ccagcatttg ttcacaagta
    18721 cgttgcccgg tgatcgggtg ataaaaataa atcgctcctt cggtttctga cgtgatggtt
    18781 tcaagaaaat ttttcatcac atcacgcacc gcatcccaac gttgcacaca catcgctaac
    18841 gtcataactt cttgtggcag tacagatttg accggcgctt tgacccgcat ccccatacga
    18901 aacgtcaacc aataagccgg cagaccgatg attttaggta aaacggtttt tttatgaaga
    18961 atggcttccc gatgatgcaa ttgtttggcc atgccttcct cgactaaaac cagatgttgc
    19021 aatacatcca gtgcatgcca cgcatcgagt ttaggtcgcc aatgcgattg atgttcattc
    19081 atagcctgca cacaggtcac aaaataagtt ttgctcgcct caatgcggga ccatttggaa
    19141 gataaactta caatcatagg tttcgtgcgt tttgccgttc aaaaatcata cgattatatc
    19201 gcgatctgac gtagattaaa agagtttttt tattctacaa tatacccggt gtttttttag
    19261 aaaatttttt gtaataaata tagctctata tgtactactc ttatcaatat gggtaatc
//
LOCUS       DAOQGF010000060        19006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1340_length_19006_cov_6.016780, whole genome shotgun sequence.
ACCESSION   DAOQGF010000060 DAOQGF010000000
VERSION     DAOQGF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_19006_cov_6.016780"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK937_09445"
     CDS             <1..315
                     /locus_tag="PK937_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH32835.1"
                     /translation="IGDIGGGEPGMDEFVPVEQQPVIVAKVQPVYPELAKKAGLEGKV
                     IVKALIDESGTVTKCVVLQGDEMFKESAVNALMSMKFKPAINGNRPVKVWITYPFTFR
                     LK"
     gene            complement(372..1730)
                     /locus_tag="PK937_09450"
     CDS             complement(372..1730)
                     /locus_tag="PK937_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32836.1"
                     /translation="MKSFVKYFFETNASEVTESKKGLHISLSKPLRDVFGKEKLDLVF
                     DTKDLDESADLVTHGSYVLNTIYEFLQETGTKAVSRLKEKHEISRESIARQIHIQNAS
                     INHVKTKKQKVADILFNFRIGYLSDEKSEDIFTLGIDHRGHVFDARSYYTDEVIKHDL
                     ETLSNKSGIEISQKEIEVAFRTCLKEVSTRAQEHGQGLQKEILKRLHRNVTRIKGYYT
                     AQIDEMHRSQTSYEEKRLTIEREYQHKLKEEIENHKLRIVLKLLSYHIIERTEIEVTT
                     TVEHNKTGHKAEVPLLYDSYHGLLDYGHCPSCKLPIQNMTLLADGKIGCSRCVFVCSG
                     CKDEFPDKEHAHHCMVCNTELCTNCNTHCHDCGHTVCETHSTVCFADKEIVCDQCVKS
                     CSDCGRTMCSDHTFLCHATKVPICHEHRVICPKCRKIYARGFIDKQKKNDRKCPECLT
                     TL"
     gene            complement(1880..2206)
                     /locus_tag="PK937_09455"
     CDS             complement(1880..2206)
                     /locus_tag="PK937_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNH32837.1"
                     /translation="MRLTILTLAAILISSCGKEEKLDKAVVKVNSAICGECSGTITKA
                     VMAVNGVKKVEVDTDTKIATISYVPGSVKIGDIESAIVKSGYDANDQKADPAAYEQLP
                     DCCKKK"
     gene            complement(2208..2501)
                     /locus_tag="PK937_09460"
     CDS             complement(2208..2501)
                     /locus_tag="PK937_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32838.1"
                     /translation="MSANLKKTNSTKLQQAKKSPVKKATLPFTAENYYIFFAGIITII
                     IGYVCLATPPVYGTVSMTIAPILLVLGYLVIIPVAILYRKKEEVQSIESTEGI"
     gene            complement(2550..3359)
                     /locus_tag="PK937_09465"
     CDS             complement(2550..3359)
                     /locus_tag="PK937_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNH32839.1"
                     /translation="MKFTLGYSTCPNDTFIFDAMVHKKIDTEGIEFEPFLADVEELNQ
                     KAFRCELDITKLSYHAFLYLLKDYALLTSGSALGHRCGPLFISARPINVHGIAGDKIL
                     IPGKLTTANFLLQFFLGKTVQAQPVLFSDIEKNLLNGSAEAGVIIHEGRFTYQAKGLH
                     LIQDLGDYWETKTGMPIPLGAITIRKTFGPEIQSVIGRIIRRSVEYAFSHPTSSKSYV
                     KHHAQELDDTVIAEHIKLYVNDYTLDLGTKGYAAVDFLKKEALRIGILPSS"
     gene            complement(3362..4054)
                     /gene="mqnB"
                     /locus_tag="PK937_09470"
     CDS             complement(3362..4054)
                     /gene="mqnB"
                     /locus_tag="PK937_09470"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNH32840.1"
                     /translation="MRILIVSATEAEIKPLLVHYKAVHPDKLGLWRSLTSHSHTIDFL
                     VTGIGMINTAYSLGKQLQISSYDWAINLGIAGAYSEVHALASIVRVDTDILAEMGATT
                     ADGQFENLQRMGFPLHSYDNKAIYNEIENPNDLQNWSRLAQTPKVRGITVNTVTGNTE
                     RILWNRNNFTPDIESMEGGAFALACLRAGQPYVQYRSLSNFVEPRDRSRWKINEAVAA
                     LSTFTIQLLEKI"
     gene            complement(4108..5313)
                     /gene="mqnE"
                     /locus_tag="PK937_09475"
     CDS             complement(4108..5313)
                     /gene="mqnE"
                     /locus_tag="PK937_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNH32841.1"
                     /translation="MKHINTVYDHSLIKSMLHTSGLDAIYQKVMDHERLSFEDGVQLF
                     ETRDLTSVGYMANIVRERMHGDRSFFVRNRQLNPTNVCEYSCMFCSFYRREGEEGGYT
                     MTMEQIAQRVKDGLKQGIQEIHITSGLHPGLPFSYYTDMLSTIKSVAPHIHIKGFTAV
                     EIEYYANHYNMSMRAVLEALMKAGLEAMPGGGAEIFAQRVREKICDEKATAEGWFKVH
                     GMAHELGLMTNATMLYGHIERYDERIDHLIRLRELQDDSLAKKTKGSFSTFIPLKYQH
                     ENNRLKKLPQVSAFEDLKVVAVSRLMLDNFPNIKAYWVVIGKNMAQTALSYGANEIEG
                     TILEETIMSMAGTDSASGMTTSELIKLISDAGRQPIERDALYNVIKEFSTEDIAHAHR
                     NPVMLPVLN"
     gene            complement(5415..6506)
                     /locus_tag="PK937_09480"
     CDS             complement(5415..6506)
                     /locus_tag="PK937_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNH32842.1"
                     /translation="MNKRYHFIDLFRGWAVLVMIETHVFNAWMDKSLRSTEWFSYLNF
                     INGFVAPSFLFVAGVSFVMVAWNNWYSFTSFNPGYRKQLRRFFVIMLIGFALHLPRLD
                     WSQGWPHIVTEDLVSFFQIDVLQTIAISLILLQLLMLWTRGITVFFIVLFIITWSALL
                     ITPFLWQKDFSSSVHPLIANLLNGKNNPLFPLFPWCVFLWSGGLVGGLFMRYSTSDKE
                     HKIVPAIALIGVSLFAIGYISDISPIRFFEYESFWLTSPNWVLMRLGILLILFSFFWL
                     LDYKNWHTSPQVLLFGRQSLFAYISHLVLIYSITGEKASISIFDQNLPPWLTLILWIV
                     VCAAVYALTHLWVQLKPGLTARFKPSVKK"
     gene            complement(6527..7597)
                     /gene="prfA"
                     /locus_tag="PK937_09485"
     CDS             complement(6527..7597)
                     /gene="prfA"
                     /locus_tag="PK937_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNH32843.1"
                     /translation="MIDQLEKASIRFNEINGLLSRPETVSNNNLFRSLSKERSALEPI
                     VEKFETYKKVNSDILGNKDILHNSDDADLRDMARAEMNDLESKKQILESELKYLLIPK
                     DPHDDGDAILEIRAGTGGDEAGIFAGDLYAMYQYYVEKKGWALTLVDFTEANQGGFKE
                     IIFEVNGTGAYGKLKFESGVHRVQRVPKTETQGRVHTSAATVAVLPVLEEDDSEVVIN
                     PNDLRVDIYRSGGKGGQNVNKVETAVRLTHLPTGIVVQCQDERSQLKNREKAMKVLKS
                     RLYDLRKREHDAKIASQRKSMVGSGDRSEKIRTYNFPQNRLTDHRIGLTIYDLEGVMG
                     GKIDDIVEKLQLAENEELLKAG"
     gene            complement(7609..8586)
                     /locus_tag="PK937_09490"
     CDS             complement(7609..8586)
                     /locus_tag="PK937_09490"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNH32844.1"
                     /translation="MSNNPITPKTITVGGQAVIEGVMMRSADAVATAVRKPNGQIVIR
                     REPFTSLLHRYRFLNIPVLRGSIAIIEMLIIGTSALQYSADVSMSNDEEGSSPKKSSR
                     LELFLTTAFALGIGVLIFFAGPISLTNYFIGADQNAWSFNLVAGTIRAAMFITYVWAI
                     SQMKDIFRLFQYHGAEHVSIYAFENKDALIPENGLKYDTPHPRCGTSFLMFVMIASIL
                     SFAIIDSLYIAYIGPLGLWNRLALHLPCVPIVAGLSYELIKLSGKYYHLSWARIFMMP
                     GLWLQRITTKKPDITHIEVAMAALQSALGEEAVRAYNAGQVTGAMSVHE"
     gene            complement(8727..8948)
                     /gene="rpmE"
                     /locus_tag="PK937_09495"
     CDS             complement(8727..8948)
                     /gene="rpmE"
                     /locus_tag="PK937_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNH32845.1"
                     /translation="MKPGIHPTYPVSNVTCSCGNSFKTRSTVGDIRVEICSNCHPFFT
                     GQTKLIDTAGRVEKFNKKFAKYQDAQKKA"
     gene            9177..10394
                     /locus_tag="PK937_09500"
     CDS             9177..10394
                     /locus_tag="PK937_09500"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNH32846.1"
                     /translation="MTHADLVTKLVQKTDSKIVLLVMDGLGGIRTKQNPKTELERANI
                     PNLHTLAKESVCGRIMPVSYGITPGSGPAHFSLFGYNPADDNIQIGRGVLEALGLDMK
                     LQPGDVATRCNFATMDTKGLLTDRRAGRIPSEECERLCAMLGAKIKKIEDVKVIIKPG
                     EQYRFVVVFRGKRLHGNILDTDPQVTGAKPLKAVPQDKASTRMSKIADKFIAAANKIL
                     KDETKANTLLMRGFSGMPHVPSMSERFGLAPACIASFPLYRGVASVVGMEILPTGMDI
                     ADEFQTLKENWDKHDFFFIHIKKTDSYGEDGNSEGKMHIMEEVDKHIPMLRELGPAVI
                     MVTGDHSTPPPMKSHSWHPVPFLLHSDVCSFDDANAFTETECSKGQLGNFPSYYIMEL
                     ALANAGKLKKFGA"
     gene            10415..11335
                     /locus_tag="PK937_09505"
     CDS             10415..11335
                     /locus_tag="PK937_09505"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNH32847.1"
                     /translation="MEASWYIPDLLPTMGASEAMQRIMRHRWISWKSLCDAVKENQSA
                     EQIHDFRVASRRLRQAMACLVFYSSSAAKLKHHNHAIKTLADHAGEVRDSDIMLHKVQ
                     ILKSKHSKIQSANWDYIENQIETKRLRSLKQFQNTIQKTDLNIDISWVSSESETKNDP
                     IGVTAVDLAVQWIKERSEALWITESDCGDPLSSGTLHAFRIEAKRLRYSLEMWSGILD
                     GNISRTLKILKKLQDYLGLIHDCDTIIKTLRKSAQIYKTENEIHNAMIIKTIHYVVRE
                     RISITHRFYSYWNSDVRRVLQKQINHFVME"
     gene            11339..11836
                     /gene="sixA"
                     /locus_tag="PK937_09510"
     CDS             11339..11836
                     /gene="sixA"
                     /locus_tag="PK937_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNH32848.1"
                     /translation="MDVFLMRHGFAEDVGGKIKTDFDRPLTGEGVKLMQAEARGLKRL
                     DIRFNVIMCSPLVRAKQTAEAVAEVLGHKKVVVCDGLAIPASTAELMQAFKPFQMDYS
                     VLLVGHMPDVGRLAGFFTGVPRLAIPFKKGAICKIEVERIHPSPSGEIRWFMTPRQLK
                     FIGES"
     gene            11836..13425
                     /locus_tag="PK937_09515"
     CDS             11836..13425
                     /locus_tag="PK937_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNH32849.1"
                     /translation="MARVVSQLKKMIFAERNSKKHIAAIDIGTNSFHLIVVELLGNGK
                     YRIVDREKENVRLGSGSSDMKMIAPDAIERGVTALKRFSEIAKVYKAPIRAVATSAVR
                     EALNQAQFVKKIKDATGINIEVVSGNEEARLIYLGVLQALPVFNHKIIAMDIGGGSVE
                     YIVGARGEAQYLNSLKIGCIRLTQRFFDKGVIKPKKIQECREYIRGFVSAVAREVRDR
                     KIEFAVGSSGTILNLANMIRTNRSGISSDAQLNNFSFTKKELDRVVQNILKAKTVAQR
                     IKIIGLDIKRADIIVAGALVLQESFDLLKIKQMVVSEYALREGIIHDYINSRTNTGGH
                     HPLSNIRYRSVMHLAENFHYDAEHAHQVTRLALSIFDQTVKIHKLGSTEREFLEFAAI
                     MHEVGFFVSHAQHHRHSYYLIRNAELAGFTDQEKEIIANIARYHRKSHPKSKHEGFVN
                     LSLENQRLVCKLAGVLRIADGLDRTHSQSITSLRCKIQPKHILIKAHCKSAGTLEQWA
                     VNMKKGLFEESLGRSVTVRIN"
     gene            complement(13434..14690)
                     /locus_tag="PK937_09520"
     CDS             complement(13434..14690)
                     /locus_tag="PK937_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNH32850.1"
                     /translation="MAQGQIRYNINPTPSLGGKVFVFFNGTANYAKSTPGNAANDREN
                     LEQRVIDIINNASTSLDMAAYELNSLNIVVALCKAHERGVRVRIIMDDEAAPNNNEEL
                     WKYVRTLLSYRYQVPWMTDAGWPIVKSKKNLFKARTAQMHNKFVVADYLSADTTDDMV
                     LTGSYNFTITGMVSMQNVVRIQNAKAAQIYTEEFENMWGSDTEQPDTTKAAFHQYKGA
                     LKNDPIVFGGKIGVYLAPMNKHKTRPNYLEMIADIITREVQHDIKICSFSFSTKIDVD
                     EAIKEKAEERGVNVKAVFDRSLGKGNWSLYKSMIGDTSARVVWKKKAEAYLAYEDRQL
                     HHKYLIIDAESPDTSDVPVVITGSFNFSKNANEANDDNFLVIRDRAIANQYLQEFYAR
                     FEAAKKLELSQKETNQTEKLITDDDE"
     gene            complement(14831..16231)
                     /locus_tag="PK937_09525"
     CDS             complement(14831..16231)
                     /locus_tag="PK937_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32851.1"
                     /translation="MNSKFKNTMASNILLLFVVFAFAACSVDYPEVSEQKLPRITTSG
                     TADFTKFATVGNSLTAGYQSSGLASRFQVNSYPAIIARQLGMDVTDDSTSEFQQPLAS
                     DSGTQGILKLNFSPTGPLPSTTYAITEPFINLGLSRAYDNLAVPGAFVYDYLNASTPA
                     TGWGGLILGSANTLYTPILRGLGTQHQQLRSLHPTFVIWWEGHNDILGYATRGAGVNT
                     TPGSSQGAPPWTPRNSTEAGGMGIPFNFTTMYTASLDSVVATGADVIVGNIPYVTDIP
                     YFTTVYPSSSVFKLNNDSIVTITNALTGSKAKLYFEEDVTGPADSIVYMLLPVSSLIG
                     KTNVSGFSGKPYGLTPTARIQGRYTLTVSEVNHIKSIIDGYNSIISAAAATHGIQVVD
                     FNSYFTTIKSNGILLPNGSKIGTTFISGGLFSLDGVHPTTFGYAVVADKWIESINSKF
                     NSNVPRVNLMDYYQFK"
     gene            complement(16256..17629)
                     /locus_tag="PK937_09530"
     CDS             complement(16256..17629)
                     /locus_tag="PK937_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNH32852.1"
                     /translation="MKSLWNTRIRAFSAAALVLAALSEVYAGGFSIYEQGGRATSQGG
                     AVVARPWDASAAFYNPAGLAFYGGAPGTYRFYVGVTPVQSLSKFTGLDSFPGRGVHEE
                     AKAKWFPPFYAYGVYQINENMAAGLSITTPFGLGTEWKNPNNTYSGRFRSLMANIEAV
                     YISPVISYKVNNMIGVGAGVDFVYSRVELRRHQGIDIFDGEATKKYDVADIYLKGEDK
                     LSMGFHVSAYAKINENLSFGADYKHSIKNDYEGHARFRQIKTGYVAIDNSVAANFAND
                     LFGGMRQEGNTSVTFPSSLVIGAAYKPMEKLTVEADFALAGWSVFKEVEILFPNQRSI
                     DATTGDTIKVKSILKENYDDTWQIRLGAEYDVNERLQVRAGYIFDKTPAPTETVNPLL
                     PDANRNDFSIGVGYKINDNWHVDAAYMAVIFSERSTKGKNVDNFDGVYNSHVNLFSLG
                     FGYTFGN"
     gene            complement(17795..>19006)
                     /locus_tag="PK937_09535"
     CDS             complement(17795..>19006)
                     /locus_tag="PK937_09535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH32853.1"
                     /translation="TYYESAKEVGGDLFDFIKVDDDTIGIVVADVSGKGVPGSLVMTM
                     IRTALRLEARGNKNPADVLARVNEFVVDDMKKGMFVTMFYIVLDSRNREIHYASAGHN
                     PMILHRVSTKQTYYLNPRGFPVGISLPDRKLFGQSIQTDSIKLQEDDILIAYTDGITE
                     AMNHERDLYSEERFLETIRRHAHLDAKAFVENMRNDLNEFTQGAPQSDDITLVAIKEN
                     LAAGDVLFNLRKQLIDMVAEGMSVKEACAKTRISTSTYYKYKKKYDELGEAGLRNKSE
                     GSNIEAKHFSIEQRAKIFDVIRKHPEFGPKMISNTLNSEEYSFTTIPEKRIYDELVRL
                     RLNTEEKRQAFVSQKAKTKRFKMPGTPLLTMEGEVIKNDGSNANSIVDRIMQQRQQAI
                     ESEKKTEPAEA"
BASE COUNT         5457 a         3936 c         4155 g         5458 t
ORIGIN      
        1 atcggcgata tcggtggagg tgaaccgggt atggatgaat ttgtacccgt cgaacaacaa
       61 ccggtgatcg tggcgaaagt acagccggtt tacccggaat tggctaaaaa ggccggtctc
      121 gaaggtaaag tgatcgtgaa ggcgctgatc gacgaatccg gtacggttac aaaatgcgtt
      181 gtattgcagg gtgacgaaat gttcaaagaa tcggcggtta acgctttgat gtctatgaaa
      241 tttaaaccgg caattaacgg taatcgcccg gtaaaagtat ggattaccta tccttttact
      301 ttccgtttga aataatacgg tataaaaatt tatttaaaag gcccgttgag catatcaacg
      361 ggctttttat tttacaaggt ggttaaacac tcggggcatt ttcgatcatt ttttttctgt
      421 ttgtcaataa accctctggc gtaaatcttc ctgcatttgg ggcatatcac ccggtgttcg
      481 tgacaaatcg gtacctttgt agcgtgacat aaaaaagtat gatcactgca catcgtacgt
      541 ccgcaatcgc tgcatgattt gacacattga tcacacacaa tctctttatc ggcaaaacaa
      601 acagtactat gcgtttcgca aacagtatgg ccgcaatcat ggcaatgcgt attgcaattg
      661 gtacagagct ctgtattaca aaccatacag tgatgggcat gctctttatc gggaaattca
      721 tctttgcagc ccgagcaaac aaaaacacac cgcgagcaac ctattttccc atcagccaaa
      781 agagtcatat tttgtatcgg caatttgcaa gaagggcagt gaccgtaatc caataatccg
      841 tggtagctat catacaaaag cggcacctca gctttgtggc cggttttgtt gtgttcgacc
      901 gttgtcgtga cttctatttc agttctttcg ataatatgat aactgagtaa ctttagaacg
      961 atacgcaact tatggttttc aatttcttcc ttaagtttgt gctgatattc tcgttcaatt
     1021 gtgagacgct tttcttcata actggtttga ctgcgatgca tttcatcaat ttgggctgta
     1081 taataacctt tgatgcgcgt gacgttgcga tgcaggcgtt ttagtatctc tttttgtaaa
     1141 ccttgaccgt gttcttgtgc gcgggttgaa acctctttga ggcaggtgcg aaaagcgact
     1201 tcgatctctt tttgagaaat ttcaatcccg cttttgttgg acagagtttc gagatcatgt
     1261 tttatcactt cgtcggtata gtaacttcgt gcatcgaaaa catgccctcg gtgatcaatt
     1321 cccaatgtga agatatcttc tgatttttcg tcggacaggt aaccgattcg aaaattaaaa
     1381 agtatatcgg ctactttttg ttttttagtt ttgacgtggt ttatgctcgc attttgaatg
     1441 tgtatttggc gagcgatact ttcgcgggaa atttcatgct tttctttaag acgacttacg
     1501 gctttcgttc cggtttcttg taaaaactcg tatatcgtat taagtacata actgccgtgg
     1561 gtaaccaggt cagccgattc atccaggtcc tttgtatcaa aaaccagatc tagcttttct
     1621 tttccaaaaa catcgcgcag aggctttgaa agcgaaatgt gaagcccttt tttggactct
     1681 gtaacttccg atgcgttcgt ttcaaaaaag tattttacga atgatttcat gcttaataaa
     1741 aaaagggagc tattccataa ggtgcttact tagtgccttt cgcatgatcc gttgtcatta
     1801 tgaacgaacg gatcatgcga aggtttttac cgtaaaagag aaccttttgg aaacgctccc
     1861 taaatcgcgt taaaaagtat tattttttct tacagcagtc gggtaattgt tcataggctg
     1921 ccggatcggc tttttgatca ttggcgtcat atccggattt gacgattgcg gattcgatat
     1981 caccgatttt gacggaaccg ggaacataag agatggtggc gatttttgtg tccgtgtcca
     2041 cctctacttt tttgacacca ttaacggcca tcacggcttt tgtaatggta cccgagcatt
     2101 cgccgcagat cgcggaatta acttttacta cggctttatc taatttttcc tcttttccgc
     2161 aagaagaaat caagatggcc gctaatgtta agatggtcaa acgcatgtta tattccttcc
     2221 gttgattcga tggattgaac ttcttctttt ttgcgataca aaatagcgac ggggattatc
     2281 accaaatagc ctaaaacaag caaaatgggt gctatggtca tcgaaactgt accgtaaacc
     2341 ggcggtgttg caaggcaaac ataaccgatg atgatcgtaa taataccggc aaaaaatata
     2401 taataatttt cagcggtaaa aggtaaggta gcttttttaa caggagattt tttggcctgt
     2461 tgaagcttgg ttgaatttgt ttttttgaga ttagcagaca tattttttcc ttttgttggc
     2521 cagcaatata gatattcaac aggcgaatgt caagatgagg ggagaatgcc gatacgaagc
     2581 gcttcttttt ttaagaaatc taccgccgcg taacctttag ttcccagatc aagggtataa
     2641 tcgttgacgt agagtttgat gtgttccgcg ataactgtgt cgtcaagctc ttgagcgtga
     2701 tgtttgacgt aagatttact cgacgtggga tgggaaaaag catattccac gctgcgtcga
     2761 ataatgcgtc caatcactga ctggatttct ggcccaaacg tttttctgat ggttatggcg
     2821 cccagcggaa taggcatgcc tgtttttgtt tcccaatagt cacccaaatc ttggatcaaa
     2881 tgaaggcctt tggcctgata tgtaaaacgc ccttcgtgaa taatcactcc cgcttcggcc
     2941 gaaccgttta ataggttttt ttctatatct gaaaaaagaa caggttgtgc ctgaacggtt
     3001 tttcccaaaa aaaactgcag taaaaaattg gctgtagtca attttccggg gatcaatatt
     3061 ttatcaccgg caataccatg gacattaata gggcgtgccg aaataaataa aggaccgcag
     3121 cggtgcccca atgcgcttcc ggatgtcaat aaggcgtaat cttttaacaa atataaaaag
     3181 gcatgataac ttaattttgt aatgtctaat tcgcagcgga acgctttctg attgagttct
     3241 tcgacatctg caagaaaagg ttcgaattcg attccttcgg tgtcgatttt tttatgaacc
     3301 atggcatcaa aaatgaacgt atcattgggg caggtagaat aacccagcgt aaacttcatt
     3361 ttcagatctt ctccaaaagt tggatagtaa aggtgctcaa cgccgcaaca gcttcgttga
     3421 ttttccagcg acttcggtcg cgaggctcga caaaattgga aagcgaccga tattgtacat
     3481 aaggttgccc cgcccgtaaa caagccagtg cgaaggcgcc gccttccatg ctttcgatat
     3541 ccggagtaaa attattgcga ttccacagaa tgcgttctgt attaccggta acggtattga
     3601 ccgtgattcc tcttactttg ggtgtttgcg ccaatcggct ccaattttgc agatcgttgg
     3661 gattttcgat ttcgttgtag atcgccttgt tgtcgtaaga atgtaaggga aatcccattc
     3721 gctgtagatt ttcaaattgt ccatccgccg tcgtagcgcc catttctgcg agtatatccg
     3781 tatctacacg tacaatagag gccaaagcat gaacttcact ataggcaccg gctattccga
     3841 gatttatggc ccaatcataa gatgatattt gtaattgttt accaagacta tatgccgtat
     3901 tgatcatacc aatgccggta accagaaaat cgatcgtatg ggagtgtgaa gtaagtgagc
     3961 gccacaaacc aagtttatcg ggatgtacgg ccttataatg aaccaatagg ggtttgattt
     4021 cagcttccgt cgcggaaaca atgagtattc tcatgggata ctaaagatga attatggtag
     4081 ccgaacggtg gccaaagttt ttggaaatca attgagcaca ggtaacatga ccggattacg
     4141 gtgagcgtgc gctatatctt cagtcgaaaa ttcttttatc acattgtata acgcatcgcg
     4201 ctcaatgggt tggcggcccg catcggatat gagtttgatc aattccgacg tggtcattcc
     4261 agatgcactg tccgttccgg ccatactcat gatcgtttct tcgaggattg ttccttcgat
     4321 ttcattagcg ccatagctga gcgccgtctg tgccatattt ttaccaataa ctacccaata
     4381 agctttgata tttggaaaat tatccagcat caggcgcgat accgctacga ctttcaaatc
     4441 ttcgaaagcg ctgacctgcg gtaatttctt taatcggttg ttttcatgct ggtatttgag
     4501 cgggataaat gtactaaatg atccttttgt cttttttgcc aatgaatcat cttggagttc
     4561 acgtaaacga attaagtggt caatacgttc atcataacgt tcgatatgac cataaagcat
     4621 cgtcgcatta gtcatcaggc ccagttcatg cgccataccg tggactttaa accaaccttc
     4681 cgccgtggct ttttcatcac atattttttc acggacacgc tgagcgaaaa tttcagcgcc
     4741 accgccgggc atggcttcga ggccggcttt catcaaagcc tccagcacag cacgcatgga
     4801 catgttatag tgattggcgt agtattcgat ttcgacggct gtaaaacctt taatgtgaat
     4861 atgcggtgca accgatttga tcgtactaag catatcggta taatatgaaa aggggagtcc
     4921 cggatgtaag ccggaagtga tatggatctc ttgtatgcct tgctttaatc cgtctttaac
     4981 acgttgtgcg atttgttcca ttgtcatggt atagccgcct tcttcgcctt cacggcggta
     5041 aaacgaacaa aacatgcacg aatattcaca tacgttggtg gggttaagct ggcggttgcg
     5101 tacaaaaaat gatcggtcgc cgtgcattct ttcccggaca atattggcca tgtaacctac
     5161 gctggtcaga tcacgcgtct caaacaattg aacgccatct tcaaaagaga gccgttcatg
     5221 atccatcact ttttgataaa tggcgtccaa accggaggtg tgcagcatgg attttatcag
     5281 agagtgatca tatacggtat ttatatgttt cattaatatc tgcctttgga tatagtgttt
     5341 atcataattt aagtttcaat tttttttgaa acattggtaa actaaaataa gccgccaata
     5401 taaacaagta taaactactt tttgacggac ggtttaaatc gtgccgtaag accgggtttg
     5461 agttgtaccc acaaatgcgt cagcgcatag acggcggcac aaacgacaat ccacaaaata
     5521 agggttaacc agggcggtaa attctgatca aatatgctga tggacgcttt ttcgcccgtt
     5581 atcgagtaga taagtacaag gtgtgaaata tatgcaaaaa gagattgtcg tccgaaaagt
     5641 aatacctgtg gtgatgtgtg ccagtttttg taatcaagta accagaagaa cgaaaaaaga
     5701 ataagtaata tacccagacg catcagtacc caattcggac tggtcaacca aaaactttca
     5761 tattcaaaaa aacgaatggg cgaaatatcc gaaatataac cgatggcaaa caaagatacc
     5821 ccgatcagtg caatcgccgg tactatttta tgttccttat cagaagtgct atagcgcata
     5881 aaaaggccgc cgaccaagcc gccggaccaa aggaaaacac accacggaaa aagtggaaat
     5941 aaaggattgt tttttccatt tagtaaattg gctattaggg gatgtacgga tgaactaaaa
     6001 tccttttgcc aaaggaacgg cgtaataaga agggccgacc atgtaataat aaaaagcaca
     6061 ataaaaaata cggttatgcc gcgagtccac agcatcaata gttgtaaaag aatcaagctg
     6121 atggcgatag tttgcaatac atcaatctga aaaaagctga caaggtcctc cgtaacaata
     6181 tgcggccatc cctgactcca gtccaatcgc ggaagatgaa gagcaaaacc gattaacata
     6241 attacgaaaa aacgacgcag ttgcttccga taaccgggat taaatgaagt aaaggaatac
     6301 caattattcc aagcaaccat gacaaaggaa acaccggcaa caaaaagaaa tgatggtgca
     6361 acaaaaccgt taataaaatt caggtaggaa aaccattcag tagaacgcag gcttttatcc
     6421 atccacgcat taaaaacatg ggtctctatc attacaagga cagcccatcc gcgaaaaaga
     6481 tcaatgaagt gataacgttt attcatacgc atcgcacctg caaggattat ccggctttaa
     6541 gtagctcttc gttttcagct aattgtaatt tctctacgat atcatctatt ttgcctccca
     6601 taacgccttc caagtcatat atcgtaagtc cgatacgatg atccgttaag cgattctgag
     6661 gaaaattata cgttctgatt ttttcgctgc gatcgccgga gccgaccatg gatttgcgct
     6721 gagaagcgat cttagcgtcg tgctctcgtt tacggagatc atatagacgc gatttgagca
     6781 ctttcatcgc tttctcgcgg tttttcaatt gtgagcgttc gtcctgacat tggacgacga
     6841 tgccggtagg taagtgtgta aggcgcacgg cggtttcaac cttattaaca ttctggcctc
     6901 ccttgccgcc ggagcgatag atatcaacgc gtaaatcatt ggggttaatg acgacttctg
     6961 agtcatcctc ctcgagaaca gggagaacgg ccacagttgc tgcagaagta tgtacgcggc
     7021 cctgcgtttc agttttgggt acacgttgca cgcgatgaac gccgctttca aatttaagtt
     7081 taccgtaagc gccggtaccg tttacttcaa aaatgatttc tttaaatccg ccttgattgg
     7141 cttcggtaaa atcaaccaat gtgagcgccc aacctttttt ttcaacgtaa tactgataca
     7201 tggcatagag atcgcctgca aaaataccgg cttcgtcacc gcctgttccc gcacggattt
     7261 cgaggatagc gtctccgtcg tcatgcgggt ctttgggaat cagaagatac tttagctccg
     7321 attcgagtat ttgttttttt gattccagat cgttcatctc tgcacgagcc atatcgcgca
     7381 aatcagcatc atcggagttg tgaagtatat ccttgttacc gagaatatca ctgttcactt
     7441 ttttataggt ttcaaacttt tccacaatag gttcgagcgc gctgcgttct ttgctcagcg
     7501 atcgaaacag attattgtta cttaccgttt ccggtcgtga taaaagtccg ttgatttcgt
     7561 taaaacgaat ggatgctttt tcgagttgat cgatcatttt tttctatgtt attcgtgtac
     7621 gctcatggcg ccggtgacct ggcctgcgtt atacgcgcgt acggcttctt cgcccagcgc
     7681 cgattgcaga gccgccatag ccacttcgat gtgcgtgata tcaggttttt ttgttgtgat
     7741 gcgctggagc cataatccgg gcatcataaa aatccgtgcc caactcagat gataatattt
     7801 accggaaagt ttgatcaatt cgtatgataa acccgcaaca atcggtacac agggcaaatg
     7861 taaagcaaga cgattccaca gccccaaggg accgatatag gcgatatata aactatcaat
     7921 gatagcaaag gacaagatgg atgcgatcat aacaaacatc aaaaaactgg tcccgcaacg
     7981 ggggtggggt gtatcgtatt ttaatccatt ttcaggaatc aatgcgtcct tattttcaaa
     8041 cgcgtagata gagacatgct cggcgccatg gtactggaaa agccgaaaaa tatctttcat
     8101 ctgactgatc gcccaaacat aggttataaa catggcggcg cgtattgttc ctgctacaag
     8161 attaaaagac catgcatttt gatcggcacc aataaaataa ttggtcaagg agatagggcc
     8221 tgcaaaaaat atcaaaaccc cgatgcctaa agcaaaagcc gtggtcaaaa acaattccag
     8281 tcgggaagat tttttgggag aagagccttc ttcgtcattg ctcatggata cgtctgctga
     8341 atactgtaaa gccgaagtgc caataataag catttcaata atagcaatac tgccgcgcaa
     8401 gacgggaata ttgagaaaac gatagcgatg aagcaaagat gtaaagggtt cgcgtcgtat
     8461 gacgatttgc ccgttaggtt tccttacggc tgtagcgaca gcatccgctg aacgcatcat
     8521 tacgccttcg atgacggctt gaccgccaac ggtaatcgtt ttaggtgtta tgggattgtt
     8581 actcatttat ggtaaataaa aagggctttc ccaaaagatt ttattccata taagaaacgc
     8641 aatctgtgtt tttacaaaaa acaaattcga cgtttcgtct taggtaaaat tcttttgaga
     8701 cagccctttg tcagacgtaa tctaaactaa gcttttttct gagcgtcctg atacttggca
     8761 aattttttat tgaatttttc aacgcgaccg gcggtgtcaa tcagcttggt ttgccctgta
     8821 aaaaaaggat gacagttgga gcagatttcg acgcgaatat cgccgacggt tgaacgggtc
     8881 ttaaaactat taccgcaaga gcaggtaacg tttgacacgg gatacgtggg gtgaatacca
     8941 ggtttcatgg ctgtccttat gttaaattgt tatatatatg cttcttaagc gcgcaatagt
     9001 aaccaatgat cgtattaaaa acaatatttt ttttatatta ttcccgtttt cttgtggtta
     9061 tggtttttac acatctttta gtgcccggtt cctgacaatg aacatttgaa taatcaggta
     9121 atgttgttat atttaaaggt caaaaataat atcgttcatt ttgatgaggg taggttatga
     9181 cacatgccga tttggtaacg aagcttgttc aaaaaacaga ttctaaaatc gttctgttag
     9241 tcatggatgg gctggggggg attcgtacaa aacaaaatcc taaaacagag ttagagcgtg
     9301 ccaatattcc gaatttgcac acgttggcca aagaatccgt gtgcggacgt attatgccgg
     9361 tttcttacgg cataaccccg ggaagcggcc ccgcacattt ttcattattt ggctataatc
     9421 ccgcggatga taatatccag atcggtcgcg gcgtgcttga ggctttgggc ttagatatga
     9481 agttgcaacc gggtgatgtt gccacacgtt gtaatttcgc aacgatggat acgaaaggat
     9541 tgcttactga tcggcgcgct ggtcgtattc cctcggaaga atgcgaacgt ctttgtgcga
     9601 tgctgggtgc taagattaaa aaaattgaag acgtcaaagt gatcattaaa ccgggcgaac
     9661 aataccgctt tgttgttgta tttcgcggga aacgtctgca cggcaatata cttgataccg
     9721 acccacaagt aaccggagcc aaaccgctga aagccgttcc ccaagataaa gcgtcaacac
     9781 gtatgtcaaa aatagctgat aaatttatag ccgcagcaaa taaaatccta aaggatgaaa
     9841 ccaaggccaa tacgctgctc atgcgcggat tttcgggtat gccgcatgta ccttcgatgt
     9901 ccgaacgttt tggtttggcg ccggcgtgta ttgccagttt tcctctttat cgtggtgtcg
     9961 ccagtgtcgt gggtatggaa atattaccaa cgggtatgga tatagctgat gaatttcaaa
    10021 cattgaaaga aaattgggat aaacacgatt ttttctttat tcatatcaaa aaaaccgata
    10081 gctacggcga agacggaaat tctgaaggaa agatgcatat catggaagaa gtagataagc
    10141 acattccgat gttgcgcgaa ctcggtcctg ccgtaattat ggtaacggga gatcattcga
    10201 caccgccgcc gatgaaaagt catagttggc atccggttcc atttttactt cattcggacg
    10261 tatgtagttt tgatgatgca aatgccttta cagaaaccga atgctcaaaa ggtcaattgg
    10321 gtaattttcc ctcatattat atcatggaat tagcattggc caatgcagga aaattgaaga
    10381 agtttggcgc gtaacctata gaaataaaat tactatggaa gcttcgtggt atatcccgga
    10441 tttgcttccg acaatgggcg catctgaagc gatgcagcga atcatgcgtc atcgctggat
    10501 ttcatggaaa tccttatgcg atgctgtcaa agaaaatcag agtgcagagc agatacacga
    10561 ctttagagta gcctcgcgac gattacgtca agcgatggcc tgccttgtat tttactcttc
    10621 ttcggctgcg aaactgaaac atcacaatca tgcgatcaaa acattagctg atcacgccgg
    10681 ggaagtgcgc gatagtgata ttatgttgca taaggttcag attcttaaat ccaagcactc
    10741 aaaaattcaa tctgcgaatt gggattatat agagaatcaa attgaaacca aacgtttgag
    10801 atcattaaag caatttcaaa atacaataca gaaaacggat ttaaatatag acatatcatg
    10861 ggtttcttca gaaagcgaaa ctaaaaacga tcccatagga gtaaccgcag tggacttagc
    10921 ggttcagtgg ataaaagaaa gaagtgaagc gttgtggatt accgaatcag attgtggcga
    10981 tccattatca tccggaacgc tccatgcttt tcgaattgaa gccaaaaggt tacgttattc
    11041 gctcgaaatg tggagcggga ttctggacgg taacatatct cgcacattaa agattttaaa
    11101 aaaactacaa gattatctcg gactaattca cgattgcgat acgataataa agacacttag
    11161 aaagtcagcg caaatttata agacagaaaa tgaaatacac aacgctatga taatcaaaac
    11221 gatacactat gtagtacggg aacgtatttc gattacccat cggttttatt cgtattggaa
    11281 tagtgatgtg cgccgtgttc tgcaaaaaca aattaaccat tttgtcatgg agtaagttat
    11341 ggatgtattt ttaatgcgac acggtttcgc tgaagatgta ggggggaaga ttaaaaccga
    11401 ttttgatcgg ccgttgaccg gcgaaggtgt caaattgatg caagctgaag cacgcggcct
    11461 caaaagactg gatatacgtt ttaatgtcat tatgtgcagc ccattggtac gagccaaaca
    11521 aacggcagag gctgttgccg aagtattggg acacaaaaag gtggtcgtat gtgatggttt
    11581 ggccattcct gcgtcaacag ccgaacttat gcaggcattc aaaccgtttc aaatggatta
    11641 cagcgtttta cttgtgggcc acatgccgga tgtcggaaga ttagccggtt tttttacggg
    11701 agtaccgagg ttagctattc cttttaagaa aggtgcaatc tgcaaaatcg aggtggaacg
    11761 catccaccca tcgccgagcg gtgaaatacg ctggtttatg acgccgcgtc agctcaagtt
    11821 tatcggagag tcctgatggc acgtgttgtt tcgcaactca aaaaaatgat attcgctgag
    11881 cggaattcca aaaagcatat tgctgcgatc gatattggta ctaattcatt tcatctcata
    11941 gttgttgagt tattgggtaa cgggaagtat cgtatcgttg acagggaaaa agaaaatgta
    12001 cgtttgggca gcggtagctc tgacatgaaa atgattgctc cggatgctat agaaaggggc
    12061 gttacggcgt taaagcgctt ttctgaaatt gctaaagtat acaaagcgcc aatacgcgcc
    12121 gtagcaacca gcgccgtgcg cgaagcgctc aatcaggcgc aatttgtaaa aaaaataaaa
    12181 gatgcaaccg gcataaatat tgaagttgtt tccggcaatg aagaagcacg gttgatttat
    12241 ctaggggtgc tgcaggcatt gccggttttt aaccataaaa ttatcgcaat ggatatcggt
    12301 ggcggcagtg tcgaatatat cgttggagct cgaggagaag cgcaatatct gaacagcctt
    12361 aagatcggat gtattcgttt aacacagcgg tttttcgata aaggtgttat taagcctaaa
    12421 aaaatacagg aatgccgcga atatattcgc ggatttgtaa gcgccgtagc acgggaagtc
    12481 cgtgatcgca aaatcgagtt tgcagttggc agttcgggta caattctcaa tttagccaat
    12541 atgatcagaa ccaatagaag cgggatttca tcggatgccc agttgaataa tttttctttt
    12601 accaaaaaag aactggatcg tgtagttcag aatatcttaa aagcaaaaac ggtagcgcag
    12661 cgaataaaaa tcatcgggtt agatattaaa agagcagaca ttattgtggc cggtgctttg
    12721 gttttacagg aatcatttga tttacttaag atcaagcaaa tggtagtttc cgaatacgcg
    12781 ctgagagaag gtattatcca tgattacata aacagccgca ccaatacggg tgggcatcac
    12841 cccttatcaa acattcgtta tcgtagtgtc atgcatctgg ctgaaaattt tcactacgat
    12901 gcagaacatg cacatcaggt aacccgattg gcgcttagta tttttgatca gactgttaaa
    12961 atacataaac tgggcagcac ggaacgggaa ttccttgaat ttgcggcgat catgcacgag
    13021 gtgggttttt ttgtctcgca cgcacagcat catcgccatt cgtactatct tattcgaaat
    13081 gcggagttgg cgggatttac cgatcaggaa aaagaaatta tagccaatat agcgaggtac
    13141 catcgcaaga gccatcccaa atcaaagcat gaaggttttg tcaatctttc actcgagaat
    13201 cagcgcttag tatgtaaatt agcaggtgtg ttgcgtattg ctgacggatt ggataggacg
    13261 catagtcaaa gcattacgtc gcttcggtgt aaaattcagc ctaaacatat tttgatcaaa
    13321 gcgcactgta aatcggcggg aacgctggaa caatgggcag ttaatatgaa aaaaggattg
    13381 tttgaagagt cgttagggcg ttctgttact gtgcgtatca attagcagca tctttattcg
    13441 tcgtcgtccg tgatcagttt ctcagtttga ttagtctctt tttgtgaaag ctcaagtttt
    13501 ttggccgctt caaaacgtgc ataaaattcc tgtaaatatt gattggctat cgcacgatca
    13561 cgtatgacca aaaagttatc atcattggct tcattggcat ttttgctaaa attaaaagaa
    13621 cccgtaatta cgacggggac atctgacgta tcaggggact cagcgtctat gatcaaatat
    13681 ttatggtgca attgacggtc ttcatatgcc aaataggcct ccgccttttt tttccaaacg
    13741 acgcgcgccg acgtatcacc gatcattgac ttatataaac tccaatttcc tttccccagc
    13801 gacctatcaa atacagcttt tacattgacg ccgcgttcct cggctttttc ctttattgcc
    13861 tcatcaacat cgattttagt actaaacgaa aacgaacata ttttgatgtc atgttgcacc
    13921 tcacgagtaa ttatatctgc aatcatttcc agataattag ggcgcgtttt atgcttattc
    13981 ataggcgcca gatatacgcc gatttttccg ccaaaaacaa tgggatcgtt ttttaaagca
    14041 cctttgtatt gatgaaaggc ggctttcgtc gtatccggct gttctgtatc ggagccccac
    14101 atgttttcga attcttcagt ataaatttga gcagcttttg cgttttgaat tcggacaaca
    14161 ttttgcatgg aaaccatgcc tgtaattgta aaattatagg agccggttaa taccatatca
    14221 tcagtcgtat cggcgctcaa ataatcggcc acaacaaatt tattgtgcat ctgtgccgta
    14281 cgtgctttga agagattttt tttggattta acgatgggcc agccagcatc agtcatccag
    14341 ggtacctgat accgataact cagcaaagtt cgtacatatt tccaaagttc ttcattattg
    14401 ttcggcgcgg cttcgtcatc catgataata cgtacgcgca caccccgctc gtgagcttta
    14461 catagagcca cgacgatatt aaggctgttc aactcatagg ccgccatatc taagcttgtt
    14521 gatgcattat tgataatatc aatgacccgt tgttcaagat tctctcggtc gttggctgca
    14581 tttcccggag tgctttttgc ataattggcc gtaccattaa aaaaaacaaa aaccttgccg
    14641 cccaaagaag gggtagggtt gatattatag cgaatttggc cctgtgccaa agaaaaatgg
    14701 accacaagga ttgaaactac aaatttagcg tttctattaa acatgaaaca aaatattcaa
    14761 tcagcacatg atacacaaat aaaaaacccc gtttcatcag aaacggggtt ttttattaag
    14821 gataccagta ttacttgaat tggtaatagt ccatcaggtt tacgcgtgga acattactgt
    14881 tgaatttgga attgatggat tcaatccatt tatccgcaac gacggcataa ccgaaagtcg
    14941 tagggtgaac accatccaaa ctgaaaagac cgcccgatat gaaagtcgtt ccaattttgc
    15001 taccgttggg tagcaaaatt ccattgcttt taatggtagt aaaatatgag ttgaagtcaa
    15061 cgacttgaat accgtgggtt gctgcggctg ccgagattat cgaattatag ccatcaataa
    15121 ttgattttat gtgattgact tctgatacgg tcagggtata acgtccctga attcttgcgg
    15181 tgggagttaa accgtaaggc tttccgctaa atcccgaaac attggtctta cctattaaac
    15241 tacttaccgg taacaacata taaacaatac tatcagcagg ccccgttaca tcttcttcaa
    15301 aatacaattt agctttagag ccggttagag cgttcgtaat ggtaacgatc gagtcattgt
    15361 ttagtttgaa aacactcgat gaagggtaaa ctgtagtaaa ataagggata tctgtcacat
    15421 agggtatatt tcctactata acatccgcac cggtcgcaac aacactatcc aatgaagcag
    15481 tgtacatcgt cgtaaaattg aaaggaatgc ccattccgcc agcttcagtc gaatttcttg
    15541 gagtccaggg cggagcgcct tgagatgagc ccggtgtagt atttacgccg gcgccacggg
    15601 tagcgtaacc taaaatatca ttgtggccct cccaccaaat aacaaatgta gggtgcaacg
    15661 aacgaagttg ctgatgttgt gttcctaatc cgcgtaaaat tggagtatat aatgtattgg
    15721 cgcttcccaa gatcaagcca ccccaaccag tagccggtgt gcttgcattt aagtaatcat
    15781 atacaaaagc tccgggaaca gcaagattgt cgtacgccct acttaagcct aaattaatga
    15841 aaggttcagt aatggcatac gttgtgcttg gaagtggtcc agtaggcgaa aagttaagtt
    15901 tcaggatgcc ttgcgtaccc gaatccgaag ctagaggttg ctgaaattca gaggtcgaat
    15961 cgtctgtaac atccataccg agttggcgag caatgattgc agggtagctg tttacttgaa
    16021 aacgactagc tagtccacta ctttgataac ctgcagtcaa actgttacct acggttgcga
    16081 attttgtgaa atcggctgtg ccgctggtag ttattctcgg aagtttctgt tcgctgactt
    16141 ccggataatc aacggagcag gcagcaaaag caaaaaccac aaacagcaat aaaatattgc
    16201 ttgccattgt gtttttaaat tttgaattca tagtgactcc aatatgactt ttttattagt
    16261 ttccaaatgt atatccgaat ccgagcgaaa acagattgac atgagagtta taaactccgt
    16321 caaagttgtc aacattttta cctttggtac tgcgttcgct aaaaataacg gccatatagg
    16381 ccgcatctac atgccagtta tcattaattt tatagcctac accgattgaa aaatcattgc
    16441 ggtttgcatc tggcaaaagg ggattaaccg tttcagtcgg ggcgggtgtt ttatcgaaaa
    16501 tatacccggc acgaacttgt aaccgttcat ttacgtcata ttcggcacca agacggattt
    16561 gccatgtatc atcataattt tcttttagga tggattttac ttttatcgta tcacctgtgg
    16621 ttgcatcaat tgaacgctga ttggggaaga gaatttcaac ttccttaaat acgctccaac
    16681 ctgctaatgc aaaatctgct tccacagtaa gtttttccat cggcttatag gcagctccga
    16741 tgacaagtga gcttggaaat gtcaccgacg tattgccttc ttgtcgcatc cctccaaata
    16801 aatcattggc aaagttggca gctacactat tatcaatagc tacatagcct gttttgatct
    16861 gcctgaatcg ggcatggcct tcataatcat ttttgatcga gtgtttatag tctgcaccga
    16921 atgataaatt ttcatttatt ttagcatatg cagaaacgtg aaaacccata gataatttat
    16981 cttcgccctt cagataaata tcggcaacat cgtacttttt agttgcctca ccgtcaaaaa
    17041 tatcaatgcc ctgatggcga cgaagttcga cgcggctata aacaaaatca actccggctc
    17101 ctacgccgat catattgttg actttatatg aaataactgg gctgatatat acagcttcta
    17161 tattagccat aagtgaacga aatcggccac tatatgtatt attggggttt ttccactctg
    17221 ttcctaaacc gaacggagtt gtgatagata aaccggcggc catgttttca ttgatctggt
    17281 aaacgccata ggcataaaac ggaggaaacc attttgcctt cgcttcttca tgtacaccgc
    17341 gtccgggaaa gctgtccaaa ccggtaaatt ttgataatga ttgtacgggc gtaacgccga
    17401 cataaaaacg atatgttccg ggggcacctc cataaaatgc taaaccggct ggattgtaaa
    17461 acgccgccga agcgtcccat gggcgagcga ctacagcgcc accttgggat gtggcccgac
    17521 caccttgctc ataaatcgaa aaaccgccgg cgtaaacctc cgataaggcg gctaacacca
    17581 gagcagccgc actgaacgcc cggatacgtg tgttccaaag ggatttcatt gggatcctcc
    17641 tgaatttcct tgttattgga aagtgaaagt aattgaacaa aaatctaaag aactgaaaaa
    17701 acgaatggat tacaagacaa gtattataaa acaatactaa cgaaacagtg aaaattagtc
    17761 aatacaattc tatgttttct aaaacataca gtgtttacgc ttcagccggt tccgttttct
    17821 tctcagactc aatggcttgt tgccgctgtt gcatgatccg atcaacgatg gagtttgcgt
    17881 tagatccgtc gttcttgata acttcaccct ccatggtaag aagtggtgtg ccgggcattt
    17941 tgaaacgctt cgtttttgct ttttgactga caaaagcctg acgtttttct tccgtgttga
    18001 gacgcaggcg aactaattca tcgtagatgc gtttttcggg tattgttgta aaactatatt
    18061 cctctgaatt gagtgtgttg ctgatcattt tagggccaaa ttcagggtgt ttacgtatga
    18121 catcaaaaat tttagcgcgt tgttcgatac taaaatgttt agcctcgata ttcgaaccct
    18181 ccgatttatt gcggagtcct gcttcaccga gttcatcata ttttttctta tacttataat
    18241 aagttgaagt agatatacgt gtctttgcgc aggcttcttt cacactcata ccttcagcta
    18301 ccatatcaat caattgttta cgtaaattga aaagcacatc gccggcagct aaattttctt
    18361 tgattgcaac aagcgtaata tcgtcgcttt gcggcgcacc ttgcgtaaac tcattaagat
    18421 cgttccgcat attttcgaca aaagctttgg catccaaatg tgcatgtcgg cggatcgtct
    18481 caagaaaacg ttcttcgctg tacaaatccc gctcgtggtt cattgcttcc gtaataccgt
    18541 ctgtataagc aatgagaata tcatcctctt gtaacttaat actgtccgtc tggatagatt
    18601 gaccaaagag ctttcgatcg ggaagcgaga tgcccaccgg aaaaccgcgc ggattgaggt
    18661 agtatgtttg tttggtcgaa accctgtgaa gaatcatggg attatgaccg gcactggcat
    18721 agtgaatttc acggttacgt gaatcaagaa cgatataaaa catcgtgacg aacatgcctt
    18781 ttttcatatc atccacgaca aactcattaa cgcgggccag cacatcagca gggtttttgt
    18841 tcccgcgggc ttcgaggcgc aatgcggtac gaatcatcgt cataacgagc gaacccggaa
    18901 cacctttacc ggatacgtcg gcaactacga taccgatcgt atcatcatct acttttataa
    18961 agtcaaaaag atcaccgccg acctctttag ccgattcata gtaagt
//
LOCUS       DAOQGF010000061        18720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1374_length_18720_cov_6.582266, whole genome shotgun sequence.
ACCESSION   DAOQGF010000061 DAOQGF010000000
VERSION     DAOQGF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_18720_cov_6.582266"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PK937_09540"
     CDS             <1..232
                     /locus_tag="PK937_09540"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNH32854.1"
                     /translation="DNFSIVLRNAQSPHGQWIEETINLREDYKRIFGEYPPDQIAGLY
                     IITDSPNFQFPTTADYDHIRAQKNTTAYSCRN"
     gene            467..2182
                     /gene="rpsA"
                     /locus_tag="PK937_09545"
     CDS             467..2182
                     /gene="rpsA"
                     /locus_tag="PK937_09545"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNH32855.1"
                     /translation="MSQTTNQARDKFHGKAPLPFVKVNEAIGDYSESEFLDLKTMYDR
                     TLSEISQSTIVKGKIRAINEKEVKVDIGFKSEGIIATTEFGSNLDKYKVGDEIEVYLD
                     NMEDKEGNVILSHKKADFMRSWEKINRLHDSGETVSGTIAKRIKGGMVVSVMGIDCFL
                     PGSQIDVKPIRDFDAYVGREMNFKVVKINNLRKNVVVSSRVLIEESTKGQRERILTDL
                     QKGQILEGTVKNITDFGAFIDLGGVDGLLHITDMSWGRVKHPSEIVKLDEKIKVMVID
                     FNEHKDRISLGLKQMQPEPWLEAAKKYSIGSMVKGKIVSITDYGAFIEIEKGIEGLIH
                     ISEMSWNQTIKHPSQMFNLGDEVEAEVVNIQADQRKISLSIKKLQNDPWEHVDDRYPL
                     GSKHAGTVRNLTNFGVFVELEPGIDGLVHISDLSWTRKIRNPNEMVKKGDHLDVVIMG
                     IDKDNRRISMSHKHVTENPWDGFIQAYAASTETTGKIMRHIEKGVIVELPLGVEGFVP
                     TSHLPKSDKKDAYGEGVELNLVVIEFDKDNKKIVLSATELEKQKEAKMVEEYNASQKG
                     GEAQA"
     gene            2258..2902
                     /locus_tag="PK937_09550"
     CDS             2258..2902
                     /locus_tag="PK937_09550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNH32856.1"
                     /translation="MSSHPLLKYETECWEHGHPHVAGVDEAGRGPLAGPVVVAGAIFE
                     RDFVIEGVTDSKQLTEERREFFFNTIQSSALAFHIEIIDHEKIDKINILQASLLGMRM
                     CVEKMRIPADFVLIDGNSEAFPKNNTYHTRQRPIVKGDAKSFTIAAASILAKVTRDRL
                     MVEYDKIFPQYGFAKHKGYPTADHIQALKKHGLCMIHRKTFCAGIMNEQMGLEF"
     gene            complement(2899..3675)
                     /locus_tag="PK937_09555"
     CDS             complement(2899..3675)
                     /locus_tag="PK937_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNH32857.1"
                     /translation="MNSEIKFHCKDQGSGVPVVFIHGYPLDHSIWQNQFELSDTYRIF
                     ALDLPGMGKTPFYELETIADYARWVNLCLRQKKIVRYHLLGHSMGGYIALEMLRQDDT
                     ADILSFTLVNSQPFADTQEGVQNRYAMAERIEKEGGAFVVDMMKIKMLSANQPHLSGA
                     IAPIMAQADFRAMVQGQRAMARRDDNTDMLIQSRVPKLIITGTDDLLVSEERRKKMGE
                     LDQNIEFHMLQASGHLSMMEKPEEVNRILRNFLRRQISIC"
     gene            complement(3702..5144)
                     /gene="proS"
                     /locus_tag="PK937_09560"
     CDS             complement(3702..5144)
                     /gene="proS"
                     /locus_tag="PK937_09560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNH32858.1"
                     /translation="MAERITKREQDYSQWYLDVIAQAQLADYAPVKGCMVIRPNGYAI
                     WELVQQSLDKMFKDTGHVNAYFPLFIPESFMTKEKEHVEGFSPECAVVTHGGGKKLEE
                     SLYVRPTSETIIWSMYKNWIHSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
                     TAHATYDDAEKEAMQMLHVYKTFAEEYMALPVYHGKKTDSEKFAGALHTYCIEAMMQD
                     KKALQAGTSHNLGQNFAKAFDVKFQDENAQLQHVWATSWGVSTRLIGALIMAHSDDNG
                     LVLPPKLAPMQVVIVPIYKEDHERTATVEAAQKLKSDLQSKGIRTHVDDRDQYRPGFK
                     FNEWEVKGVPIRVELGPKDLAAGQIVCARRDKTAKTDKITMAVNEASSRIPALLEEIQ
                     KNLFDRAKEFRDKNTIFVSDYAGLKKIVENEEGFARCFWAGSREDEAKIKEETKATIR
                     CIPMEQSGEKGRCVLTGKETNVQVIFAKAY"
     gene            complement(5327..5400)
                     /locus_tag="PK937_09565"
     tRNA            complement(5327..5400)
                     /locus_tag="PK937_09565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5364..5366),aa:Arg,seq:ccg)
     gene            5642..8968
                     /locus_tag="PK937_09570"
     CDS             5642..8968
                     /locus_tag="PK937_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNH32859.1"
                     /translation="MRGSFYSRNWRGTFVYAAMFLFALTLNLRAQVTTSAISGMVKDN
                     NGTPLPGVNIIAVHEPSGTKAGAYSRSDGRFNLPGMRVGGPYTVTASMIGYKEHKIEN
                     INLALGEEKNLTFVLVEEAVEMEGSEIVAERNPIINDARTGAATTVNELQVKYLPAVT
                     RDFNDFTKYTPQFSGNSAAGRNNRYNNISIDGAVSNDLFGLAASGTPGGQAGTAPISL
                     DAIQEFQVQIAPYDVRLGNFTGGNVNAITRSGTNKFEFSSYYNYRNENFQGESFDGVL
                     PTKNFIRTEGARLGGPIIKDKMFFFVNYDHELQEVPTNYGILGDGSSLDFPGTGSRLS
                     VKKARDIQNFLKDTVLHDPGSFGMSTIPTESYKFFARLDFNLTPEHRFTIRHNYINAN
                     QDLFRRETGTNSSSSSGGFRFSNSRYIFKNITNSTVAQLNSTLSKEMFNELTMSYQVI
                     DDHRETPGDRFPYVMIRDSVAGLSYVIGTEQFSQFNKLKQSVFEFTDNFTYYMGEHTF
                     TIGTHNEYFKFENGFLPSFNGRYDFNSFENFKRSYYGLTGNIREYNLTFALDPNDPTG
                     FNADTTDRVPLAKFSVMQLGGYIQDAFKVTPSLTITAGLRFDVPIISDKPTANDSFAI
                     NYARFYHTGGENDTTIKNAKYRTDKIASGNILWSPRIGWNWDVNGDQSLQFRGGFGIF
                     SGRNPYVWISNQYSNTGADLGTIRLTGGSTLTNMTFQKDPTKQPTAGTLGSSTINVMN
                     PKFKNPQVFRVNLALDQKLSDTWVGTVEFVGSDNINDVMFKDISLAGPQSYSPTDNRP
                     RFGTISTYTSPSSSATTSPARVLPLSKSKQYTNVIYLTNTKWGYQWNASVQLQKLYDN
                     GYFGSVAYTYGRATDMTSATSSIALSNFQFNPVKGDPNNVKTATSNYEQRHRIIVSVA
                     KEIEVAPKWKTVFSLFYSGFSGRPYSTTYNGDVNADGNTGNDLIYVPKDKNDIVLVPT
                     SNSDTRSADQIWVELDEFISGDPALRKARGKIVDRNASTEPWTNRVDFQITQELPVLK
                     ELGKFELTANVINVMNIFDNEWGQSKSIANQNDQAIQFRGIQSGTGKPVFSFTKRPDR
                     YSISTTASRWAVLVGARYSF"
     gene            9001..9606
                     /locus_tag="PK937_09575"
     CDS             9001..9606
                     /locus_tag="PK937_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNH32860.1"
                     /translation="MKKYIKIMTVAIAVSFLVPLSVVHSQIAGLDIKLGVKGGFNYSL
                     FGGKDNDQTKGKTGYNVGVFALYELMPMIAVQPELLYTLKGARTTTPGQNFEYYYQYF
                     EMPVLMRVGMDIEEQKFYFIGGPSFSYAKKAYTRDREGSATVLTKPKKSDYGIIAGLG
                     TGTADSKITVEGRIYFGVPDVVKKTKVNNITYSIGMGYLIY"
     gene            complement(9740..11323)
                     /locus_tag="PK937_09580"
     CDS             complement(9740..11323)
                     /locus_tag="PK937_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH32861.1"
                     /translation="MLIVILCCSLSLFAQAEKGDSVAAEAIKTEAIERSQALQTLNVL
                     TNVIGARLTNSKGLAAANDWTREALEKMGLSAEVEPWGEFGRGWDMKSFRAGIVAPYA
                     IPLIAYPKAWSPATRGTVRSKILYINETKLEDIQKKYSNKLKNAIVLLGDKVKIEEDF
                     EADAKRWSDSELLTMSNAGKNEGRAESFRRSFRKSPRFSAFIERQRIFTWINNQQPAL
                     IVDGGSFSFRGGTPHSGVVRVMGASMPLVDESDPFGNTNPKAWDPNPPSVAPQIAISA
                     EQYNQLIELIKSGKEVIMECDLQVEWRDKDLKAYNTIGTWSGSDRKDETVMIGAHLDS
                     WHSSTGATDNAAGVSVMMEAIRILKAIGFQPRRTIKIGLWSAEEQGLLGSAGYVNKHL
                     YDSTGAKPGYEKFNVYFNMDNGGGQIRGIYLQGHEDVQSYFRQWMEPFRSWGASTISL
                     KGTGSTDHMSFISAGLNGFQFIQDPMDYSNITWHTNMDMYDHIQPLDLKRNAAIVAYF
                     VYMSAMSDEVLPKRAIEKE"
     gene            complement(11363..12181)
                     /locus_tag="PK937_09585"
     CDS             complement(11363..12181)
                     /locus_tag="PK937_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH32862.1"
                     /translation="MRTIVMLAMCFLVIVSCSKPKDSAQRDLMRLGIAYNDQEFLSRI
                     RKGDLILTNMFLQAGMNPNMRFTNHKDSVSQGESNDPTPLMIASREGHIGIVRSLLRK
                     GADPTAVADNGQTALMYGVRYAPTELVSDLIVAGADVNGRDKDGKSALTFAVLLDRLD
                     VVRLLLDRGADPNAMDRFSFTPLMQAAIKGKAPIMEALLDKGANLEVRTPNGETVLML
                     AVNYNRMEAVRLLVNRGVDLKAKDMQGRTALSIARAKKHETIIEILTTAESKNQ"
     gene            complement(12198..12689)
                     /locus_tag="PK937_09590"
     CDS             complement(12198..12689)
                     /locus_tag="PK937_09590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNH32863.1"
                     /translation="MIIEDKKKALRFFQYGMFILTAKAQHDFQGSPYAASTVTWVSQA
                     SFEPPLIMLGLRKDSWANEAVRQTDSFALNILSEDQKSIAGKFFKNIEVNGQMINGYS
                     FDTDQSGAPILDEAQAYIECTVTDRIERGDHSIIVASIINAKVRRDFKPILLRDTGWN
                     YGG"
     gene            complement(12698..13873)
                     /locus_tag="PK937_09595"
     CDS             complement(12698..13873)
                     /locus_tag="PK937_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNH32864.1"
                     /translation="MKKPIYLDHHATTPLDPRVLQKMMPYLTEHYGNAASRTHQYGWI
                     AEEAVEQARIRIATAIGASKDEIVFTSGATEANNLAIKGIAETFQRKGRHIITVSTEH
                     ASVLDTCEYLREAGFEITVLEVSEDGILDQIKILNAIRSDTILVSVMLANNETGVIQD
                     MAAIGAITREKGIILHTDAVQALGKIPVHVENLNVDLLSLSAHKFYGPKGVGALYIRK
                     RNPRIQLTALLHGGGHENGQRSGTLNVPGIVGMGEAARFSVDELQQNQKHYAGLRERL
                     WSGLKQTIKGVRLNGPDFEKDDNFSRRLPNNLNVSIPDIDGEGLIMALKDIAVSTGSA
                     CSSAVPEPSHVLLRMGISKELAKASLRFGLGSGNTSDDIDYTIKRITETVGKLIKTI"
     gene            13987..14397
                     /locus_tag="PK937_09600"
     CDS             13987..14397
                     /locus_tag="PK937_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32865.1"
                     /translation="MKKILTFLLFTGAISVYAQEVSKQLERLDPGTATANLDNFGKTH
                     SEQITAAHQARLGELIKAGTIIIRQDTMITADKPVKIAFNWPVASGQNLKDKSVVKRD
                     PQVMTLYPAKDPSNKDIPQRWQVTYKDGTVKAIQ"
     gene            complement(14483..14677)
                     /locus_tag="PK937_09605"
     CDS             complement(14483..14677)
                     /locus_tag="PK937_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32866.1"
                     /translation="MAKKQTFGEKTDKQKRDANWKTIMIVYSSVSPKTGAWKFNERFV
                     RMPAHEDEVAFATKWLETNF"
     gene            complement(14710..15930)
                     /gene="zigA"
                     /locus_tag="PK937_09610"
     CDS             complement(14710..15930)
                     /gene="zigA"
                     /locus_tag="PK937_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HNH32867.1"
                     /translation="MTKKLPVTVLSGFLGAGKTTLLNHILKNREGKRVAVIVNDMSEV
                     NIDASLVKNGEAALSRKEEKLIEMTNGCICCTLREDLLVEVARLAKEGRFDYLLIEST
                     GISEPLPVAETFTFEDEQGESLSRYARLDTMVTVVDGYNFFNDYCGSDSLIDRNIALG
                     EEDERSIADLLIDQIEFANVIIVNKADLIGEEARKVLFSVCQRLNPDAQIVFSKYGDV
                     PLAQVLDTGLFDFDKASSAPGWLKELRGEHTPETEEYGISSFVFRARKPFHPERFWAW
                     VHTEWPGVLRSKGYFWLATRMEWAGHWEQAGGTCNYEPAGFWWSAIDRRSWPDEAAYI
                     QDIEAQMQPPYGDRRQEIVLIGKDMDKAALSQSFEKCLLTDEEMKIGHTGWTRLVDPF
                     PEWIIQSAEESVVN"
     gene            complement(16091..16402)
                     /locus_tag="PK937_09615"
     CDS             complement(16091..16402)
                     /locus_tag="PK937_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32868.1"
                     /translation="MLRYSYIVFYLIVCSILPRLHTDQCGISVHTVEITVRDLGYSTG
                     EGAQHSYCLAHQFASGHDHVDSLPLVIASIINENKGHSHVWFLESILPRRSARAPPLS
                     V"
     gene            complement(16482..17036)
                     /locus_tag="PK937_09620"
     CDS             complement(16482..17036)
                     /locus_tag="PK937_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32869.1"
                     /translation="MTRVFSKTALLTVTILMSLFVFSGCEKDDKETNPEELITSVVIK
                     FVDTSNAANTVTASFRDLDGEGGADPVQFDTIKLAANKTYHATIVLLNETEEPADTVS
                     NEVYDERNDHMFFFHGHDVNLVVNYRDVDDNNIPVGLLTRWITGAASSGEAHVTLKHQ
                     PGVKNGTSTPGETDIEVEFPIRIQ"
     gene            complement(17077..>18720)
                     /locus_tag="PK937_09625"
     CDS             complement(17077..>18720)
                     /locus_tag="PK937_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH32870.1"
                     /translation="LNGFTNNRQMAGALRLEGGLANKPGFGWRAQVSGRKAGNAQTPR
                     YVIGNSGFQEFNGAAAVGFRNHRWESQLYYSHFGTELGIYKGSHIGNTSDLIAAIKRN
                     RVPLNEDDFSFAIGNPRQVITHDLISARVHRQTDAGDRFEFQGGWQRNHRQEFDSHKP
                     LGTPAEDLKKPSFDLSLYTYTADVRWRHRPIGDFAGTIGVNVMRQRNVNQAIRLIPSF
                     HTYNAGVYWIEKWVQDDWSAEAGLRYDQRWLDASFTAQQQLKLGVNEKEDHVYRSTSA
                     AVGVNRRFAEYWSIGINAGTAWRPPGVNELYSNGVHHGTAQYEIGDPAMGVEKSYSTD
                     ITLHHNSEKINGDVSLFVNRMDNYIHLYPSRKPTLTIRGSFPTFRYAQTEARLTGIDG
                     QIQWHALDIVRLDAGWSIVRGRDLKKDEPLIYMPSDRLSGLMHWHFSDAGTIHEPYVD
                     IGITWYRRQSHVPEGAETFVNDADSVTVTPPPGYVTVDFEVGCEWHTSWSPITISLAI
                     NNVFNTRYANYLSRFRYYIDDPGRNIVLRVQIPFGNLEF"
BASE COUNT         5231 a         4473 c         3913 g         5103 t
ORIGIN      
        1 tgataacttt tcgatcgttt tacgcaatgc ccaatcgccg cacggtcaat ggatcgaaga
       61 gaccataaat cttagggaag attataaacg tattttcggt gaatacccgc cggatcaaat
      121 cgccgggttg tacattatca cggacagccc caattttcag tttccgacaa ccgccgatta
      181 cgatcatatc cgggctcaaa aaaacaccac ggcatattcg tgccgaaatt gaaaatttac
      241 ctccgtaacc cgccttccaa gccttctatt ttagatacaa attttccttg cttgcataac
      301 tctgattttc tatcttcctg cccctaaaat gggatttaat aacaacagcg tacacaagga
      361 cgcctaacca aacctccgtt tacgcggagc cagtaaaagg aaattttgcg gatgcaccct
      421 gtggtctgct catccggcgg ataaacatca gcaaataaaa gttatcatgt cacaaaccac
      481 aaaccaagcc cgtgacaaat ttcacggcaa agccccgctc cctttcgtca aagtcaatga
      541 agccatcggt gattattccg aatcggaatt tcttgacctc aagaccatgt atgatcgcac
      601 gctgagtgaa atttcacaaa gcacgatcgt caaaggtaaa atccgtgcca tcaatgaaaa
      661 agaagtcaaa gtggacatcg gattcaaaag cgaaggcatt attgcaacaa ccgaatttgg
      721 ttccaacctc gacaaataca aagtcggcga tgaaatcgaa gtttatcttg acaatatgga
      781 agataaagaa ggcaatgtga ttctttcaca caaaaaagcc gactttatgc gttcctggga
      841 aaaaatcaac cgcttacatg atagcggtga aactgtcagc ggtaccatcg ccaaacgtat
      901 caagggcggt atggttgtca gcgtgatggg tattgactgc ttcctccccg gctcgcaaat
      961 cgatgttaag ccgatccgcg attttgatgc gtatgtaggc cgcgaaatga atttcaaagt
     1021 cgttaagatc aacaacctgc gcaaaaatgt ggttgtcagc agccgcgtac tcatcgaaga
     1081 atccaccaaa ggccagcgtg aacgtatcct tacggattta caaaaaggtc agattcttga
     1141 aggaacggtc aaaaacatta ccgatttcgg tgcgtttatt gatctcggcg gtgtggacgg
     1201 attattgcac atcaccgata tgtcctgggg gcgcgtcaaa catccttcgg aaatcgtcaa
     1261 gcttgatgaa aaaatcaaag tcatggtcat tgatttcaac gagcacaaag accgcatttc
     1321 cctcggtctc aaacaaatgc aaccggaacc gtggctcgaa gccgctaaaa aatattccat
     1381 cggttcgatg gtcaaaggta aaatcgtttc gatcaccgac tacggcgcct tcatcgaaat
     1441 cgaaaaaggt atcgaaggcc tcattcacat ttctgaaatg tcatggaatc aaacgatcaa
     1501 acatccttcg cagatgttca acctcggcga tgaagtagaa gccgaagtcg tcaatatcca
     1561 ggccgatcag cgcaaaatat ccctctcgat caaaaaactg caaaatgatc cttgggaaca
     1621 cgtggatgac cgttatcccc tcggcagcaa acacgccggt acagtacgta atcttaccaa
     1681 cttcggtgtt tttgtggaac tcgaacccgg tattgacggt ttagttcaca tttccgattt
     1741 atcatggact cgtaagatcc gcaatcccaa tgaaatggtc aaaaaagggg atcatttgga
     1801 tgtcgtcatc atgggtattg ataaagataa tcgccgtatc tccatgagcc acaagcatgt
     1861 cacggaaaat ccgtgggatg gctttatcca ggcttatgcc gccagtacgg aaacaaccgg
     1921 caaaatcatg cgtcatatcg aaaagggtgt gatcgtcgaa ttgccgctcg gcgtcgaagg
     1981 ttttgtaccg acgtcgcact tgccgaaatc cgataaaaaa gacgcttatg gcgaaggtgt
     2041 agaactcaac ttagtcgtga tcgaatttga caaggacaac aaaaaaatcg tcctgtccgc
     2101 gacagagctt gaaaaacaaa aagaagctaa aatggtcgaa gaatacaacg ccagccaaaa
     2161 aggcggcgaa gcgcaagctt aatcttcagc cgtaatgaat gtgtaaaggg ttgaatagcg
     2221 taagccgttc aacccttttc ttttaaccgt ttgaactatg agcagtcatc cgctattaaa
     2281 atatgaaact gagtgttggg aacacggtca tccccacgtt gccggagttg atgaagccgg
     2341 gcgcggtcct ctggccgggc cggtcgtcgt ggccggcgca atttttgaac gcgattttgt
     2401 gatcgaaggc gttaccgaca gtaaacagct cacggaagaa cgacgggagt ttttttttaa
     2461 caccattcaa agtagtgcct tagcatttca catcgaaatc atcgatcatg aaaagataga
     2521 taaaatcaat attctgcaag cttcgctatt aggaatgcgg atgtgcgtcg aaaagatgcg
     2581 tatacccgct gattttgttt tgattgacgg caactccgaa gcgtttccta aaaacaacac
     2641 ctaccatacg cgtcagcgcc ccatcgtcaa aggtgatgcc aaaagtttta cgatcgcggc
     2701 cgcttccata cttgccaaag taacgcggga tcgtttaatg gttgaatacg ataaaatttt
     2761 tccacaatac ggatttgcca agcacaaagg ttatccgacc gccgaccata tccaagccct
     2821 caagaagcac ggactttgta tgatccatcg caaaactttt tgcgccggaa ttatgaatga
     2881 acagatggga ttagaatttt agcagataga tatctgacga cgaagaaaat tgcgaagtat
     2941 gcgattaact tcttccggtt tttccatcat gctgagatgc ccggatgcct gtagcatgtg
     3001 aaactctata ttttgatcca gttcccccat tttttttcgc cgttcttcag aaacaagcaa
     3061 atcatcggtg ccggtgatga ttaatttcgg cacacggctt tggatcaaca tatcggtatt
     3121 atcatcgcgt cgggccatcg cacgttgccc ttgcaccatg gctctaaaat ccgcctgtgc
     3181 catgatcgga gcaatcgcac ccgacaaatg cggctggttg gcggagagca ttttgatctt
     3241 catcatgtct acgacaaacg cgcccccttc cttttctatg cgttctgcca tagcataacg
     3301 attttgtacg ccttcttgtg tatctgcaaa gggttgcgaa ttaaccaacg taaaagagag
     3361 aatatcggct gtgtcatcct gacgcaacat ctccaaagcg atatatccgc ccatagagtg
     3421 tccgagaaga tgatagcgga caattttttt ttggcgtaaa cacagattta cccatcgtgc
     3481 ataatcagct atggtttcca attcataaaa cggagtctta cccatacctg gaaggtccaa
     3541 agcaaaaatt cgatacgtat ccgacaactc aaactgattt tgccaaatcg aatgatcaag
     3601 cgggtagccg tgtataaata caacgggtac accgctacct tgatccttac aatgaaattt
     3661 aatttcacta ttcatagcga tgccctctgt ttaagcgatc attaatacgc tttagcgaaa
     3721 ataacctgca cattcgtttc tttacctgtc aacacacagc gacctttttc tccggactgc
     3781 tccatcggta tgcatcggat cgtggccttg gtttcttctt tgatcttggc ttcatcttcg
     3841 cgggaacccg cccaaaagca acgcgcaaaa ccttcttcgt tttcaacgat cttttttaat
     3901 ccggcataat cgctgacgaa aattgtattt ttatcgcgaa attcttttgc tcgatcaaat
     3961 agattttttt gaatctcttc caataacgca ggaatacgtg acgaagcttc attgaccgcc
     4021 atcgtgattt tatccgtttt ggccgtttta tcacgacgtg cgcatacaat ctggccggcg
     4081 gctaaatctt tcgggcccag ttcgacgcgg atcggcacgc ctttgacttc ccattcgtta
     4141 aatttgaaac cagggcgata ctgatcacgg tcatccacat gcgtgcgaat gcctttggat
     4201 tgcaagtcgg atttcaactt ttgcgcggct tccaccgtcg cagtacgttc atgatcttct
     4261 ttatatatcg gaacaatcac cacttgcatc ggtgccaatt taggcggcaa aaccaatccg
     4321 ttatcatcgc tgtgggccat aatgagcgca ccgatcagac gggtgcttac accccagctt
     4381 gttgcccata catgctgaag ctgtgcgttc tcatcctgaa acttcacgtc gaacgctttg
     4441 gcaaaatttt gccccagatt atgcgacgta ccggcttgaa gcgccttttt gtcttgcatc
     4501 atcgcttcga tacagtaggt atgcagagcg ccggcaaatt tttctgaatc ggtttttttt
     4561 ccgtggtaaa ccggcaatgc catatactct tcagcgaatg tcttatacac atgcagcatc
     4621 tgcatagctt ctttttccgc gtcatcatac gtcgcatgcg ccgtatgccc ctcctgccat
     4681 agaaattccg tcgttcgtaa aaacaatcgg gtacgcatct cccatcgcac gacgttcgcc
     4741 cattgattga tcaaaatcgg cagatcgcga taggaatgaa tccagttttt gtacatactc
     4801 caaatgattg tttccgacgt cggtcgtaca tacaaacttt cttccagttt ttttccgccg
     4861 ccatgcgtga ctaccgcgca ctccggcgaa aaaccctcaa cgtgctcttt ttctttagtc
     4921 atgaaacttt ccggtatgaa tagaggaaag taggcattaa catggccggt atctttaaac
     4981 attttatcta atgattgttg aaccaactcc catatcgcgt aaccatttgg acgaattacc
     5041 atacaacctt tgaccggcgc ataatcggca agttgagcct gcgcaatgac atccaggtac
     5101 cactgtgaat aatcttgttc gcgtttagta attcgttcag ccataggtag attccttcga
     5161 taaaataaaa aaactgtttc gggttatcgg agacaccgga aacagctttt ggaaattagt
     5221 acgttattcg taatacgttt aaaagtgttt cctatttcaa gttgtactag gtcgattatt
     5281 gaaaaaagta aaagtctgta tttatcatgc attatgaaat aagctgtgcg cccggcggga
     5341 atcgaaccca caacctttgg ctccggaggc caacactcta tccaattgag ctacgggcgc
     5401 aaacaaaaac agcgcgtgca tagtacaaaa cgaggtttac aaattcaagg tgaattatga
     5461 atataaaaca ataaattctc ctcaaaaaaa ttcttgcgga agtaataaca aatacctaat
     5521 atttccgcaa caaatcagtc ccatattacc tcgtttagtt catttctttt aaagtatttg
     5581 ttcttaatat ttaatcaatt cttcacgatg tcccaatctc aactaataag gagaagtcag
     5641 gatgagaggt agtttttact ctcgtaattg gcgtggtact tttgtgtatg cggcgatgtt
     5701 tttatttgcc cttacactta acctccgcgc tcaggtgacc acgtctgcca tcagcggtat
     5761 ggtcaaagat aacaacggca ctccacttcc gggtgtcaat attatcgctg tgcacgaacc
     5821 ttcaggcacc aaagccggag cctatagccg ttcggacggg cggtttaacc tcccgggcat
     5881 gcgtgtcggc ggtccttata cggtcaccgc ttcgatgatc ggatacaaag agcacaaaat
     5941 tgaaaacatc aatcttgctc tcggcgagga gaaaaatctt acgtttgtat tagttgaaga
     6001 agcggtagaa atggaaggtt cggaaatcgt tgccgagcgt aaccccatta tcaacgatgc
     6061 gcgcaccggc gctgcgacta ccgtcaatga gcttcaggtg aaatatcttc ctgcggttac
     6121 ccgtgatttc aatgatttca cgaaatacac accccaattt tccggaaata gcgcggccgg
     6181 ccgaaacaat cgctataata atatttcgat agacggcgcg gtatcaaacg atctctttgg
     6241 tttagcggcc agcggtacgc cgggcggaca agccggtaca gcaccgatca gtcttgatgc
     6301 tattcaggaa tttcaagtac aaattgctcc ttatgatgtg cgcttgggca attttactgg
     6361 gggcaacgtc aatgcaatta cccgtagcgg taccaataaa tttgagtttt ctagttatta
     6421 caactatcgc aatgaaaatt ttcaaggcga aagttttgac ggtgttcttc ccacgaaaaa
     6481 ctttattcga acggaaggtg cacgtcttgg cggtccgatt ataaaagata agatgttttt
     6541 ctttgtcaat tatgaccacg aactgcaaga agttccaacc aactacggta ttttgggcga
     6601 cggttcttcg cttgattttc cgggaaccgg tagtcgcctc tctgttaaaa aagcgagaga
     6661 tatacaaaat tttctcaaag ataccgtact tcatgatccc ggttcttttg gtatgagcac
     6721 cattcctact gaaagctaca agtttttcgc acgattagat ttcaatctca caccggaaca
     6781 tcggtttact attcggcaca actacatcaa tgccaatcaa gatttattcc gccgtgagac
     6841 aggcacgaac agtagctcca gttcaggcgg tttccgtttt tccaacagcc gctatatttt
     6901 caaaaacata acgaactcga cggtcgcaca gctcaatagc actttgtcta aagaaatgtt
     6961 taatgagctc accatgagtt atcaggttat tgatgaccat cgcgaaactc cgggtgatcg
     7021 atttccttat gttatgattc gggattcggt tgctggtctt tcttacgtca tcggcacaga
     7081 acaattttct cagttcaaca agctaaaaca atccgtattc gagtttaccg acaattttac
     7141 ctactatatg ggtgagcata catttacaat cggtacacac aacgaatatt tcaaatttga
     7201 gaacggattc cttccctcat ttaatggccg atacgatttc aacagttttg aaaattttaa
     7261 gagatcctat tatggcctta cgggaaatat tcgtgaatac aatctgacct ttgctcttga
     7321 tcccaacgat ccaaccggtt ttaatgccga tacgaccgat cgcgttccgc ttgcgaagtt
     7381 ctctgtaatg cagctcggtg gttatattca agatgcattc aaagtcacgc ctagcttaac
     7441 aatcacagct ggtttacgtt ttgacgtacc tattatttca gacaaaccca ccgctaatga
     7501 cagtttcgcg atcaattacg cacgttttta tcacaccggt ggcgaaaacg atacaacaat
     7561 caaaaatgca aaatatcgca cggataaaat tgccagcggt aatattttgt ggtcaccgcg
     7621 catcggatgg aattgggacg tgaatggcga tcaatccctg cagtttcgag gtggtttcgg
     7681 aattttctcg ggtcgcaacc cctatgtctg gatttccaat caatattcca atacaggtgc
     7741 tgatctcgga accattcgtt taaccggcgg cagtactttg accaatatga cttttcaaaa
     7801 ggatcctaca aaacaaccta cggccggtac tcttggaagt tcaacgatca atgttatgaa
     7861 tccaaaattt aagaatccgc aggtattccg tgtcaatttg gctcttgatc aaaaactatc
     7921 agacacgtgg gtaggtacag ttgagtttgt aggttcggat aacattaatg acgttatgtt
     7981 caaagatatt agcttagcgg gcccacaaag ttattcacct acagataatc gtcctcggtt
     8041 tggtacaatt tccacttaca caagcccgag tagcagcgcg accacatcac ccgcccgtgt
     8101 tcttccgctt agcaaatcca aacaatacac caacgttatt tatctgacaa acaccaaatg
     8161 gggttatcag tggaatgctt cggttcaatt gcaaaaattg tatgataacg gttattttgg
     8221 aagtgtagcg tatacgtatg gccgtgctac cgatatgacc agtgcaacaa gcagtattgc
     8281 gctttctaac ttccagttta atccagttaa gggtgatcct aataacgtca aaactgcaac
     8341 ttctaattac gaacaacgcc atcgcatcat tgtatccgtg gctaaagaaa tcgaagttgc
     8401 accaaagtgg aaaacagtct tttcgttatt ctacagcggt ttttcgggtc gcccctattc
     8461 gacgacctac aatggcgacg tgaatgctga cggtaatacc ggtaatgact tgatctacgt
     8521 acctaaagat aaaaacgata ttgttttggt acctacaagt aactcggata ctcgctcggc
     8581 ggatcaaatt tgggtggagc ttgacgaatt tatcagcggt gatccggccc tgcgtaaagc
     8641 acgtggtaag atcgtagatc gtaatgcttc gaccgaacct tggaccaatc gcgtcgattt
     8701 ccagatcaca caagaacttc ctgtgttgaa agaacttggt aagtttgaat taaccgccaa
     8761 tgttatcaat gttatgaata tttttgataa tgaatggggt caatcaaaat ccatcgcaaa
     8821 tcaaaatgat caagccatcc aattccgcgg catccaatca ggcaccggca aacctgtatt
     8881 ctcgtttact aaacgcccgg atcggtattc gatcagcacg acagcgtcac gctgggctgt
     8941 gcttgtaggt gcgcgttatt ctttctaaaa cagtgtttca tcaaacaaaa ggtctaagcg
     9001 atgaaaaaat atattaaaat aatgaccgtg gctatcgccg tgtcattctt ggtgccgcta
     9061 tcggttgtgc actctcagat cgcagggtta gatatcaaac tcggcgtcaa aggcgggttc
     9121 aattactctt tgtttggcgg taaagacaat gaccagacaa aaggcaagac cggatataat
     9181 gtcggcgtat ttgctctata tgaactaatg cccatgattg ccgttcaacc cgaattgctt
     9241 tatacattaa aaggagcacg gacaaccacg cccgggcaaa acttcgaata ttattatcaa
     9301 tattttgaaa tgcctgttct aatgcgtgtc ggcatggata tcgaagaaca aaagttctac
     9361 tttataggtg gaccgtcgtt tagctatgcc aagaaagctt acacacgtga tcgtgaaggt
     9421 tcagcaactg tattaaccaa accaaagaaa agcgactacg gtattatagc cggtttgggt
     9481 acgggaacag ctgattctaa aataacggtt gaaggtcgca tttattttgg cgtgcctgat
     9541 gtcgttaaaa aaactaaagt aaataatatc acttattcca tcggaatggg ttatttgatc
     9601 tattaaactg ttctgtaacg acaaataaaa atgccggtaa tcaatattac cggcattttt
     9661 tattgaccta tcaagcggtt atgtattaaa caattaaaac tttgtgatag gtattagatt
     9721 caaaaatgaa tgtacctatt tactccttct ctatcgcacg cttaggcaaa acttcatcag
     9781 acatagcact catataaacg aagtaagcga caatagccgc attgcgtttc aaatctaaag
     9841 gctggatatg atcatacata tccatgttgg tatgccaagt gatattacta taatccatgg
     9901 gatcttgaat gaactgaaaa ccgtttaatc cggcgctgat aaaagacatg tggtccgtac
     9961 taccggtacc tttaagtgaa atggttgatg cgccccagga acggaaaggt tccatccatt
    10021 gtctaaaata agactgcaca tcttcatgcc cttgcaaata aatgccgcgg atttgaccgc
    10081 cgccattgtc catgttaaaa tagacattga atttttcata ccccggttta gcgccggtcg
    10141 aatcatataa atgtttatta acataacctg ccgagccgag taaaccctgc tcttccgctg
    10201 accagaggcc gattttaata gttcgacggg gttgaaaacc gatagctttg agaatacgaa
    10261 tagcctccat cattacggaa acgccggcgg cattatccgt cgctccggtg ctggaatgcc
    10321 aagaatccag gtgcgccccg atcatcacag tttcatcttt gcgatcactt ccgctccaag
    10381 tccctatcgt attatacgct ttcaaatctt tatcacgcca ctcgacttgg agatcacatt
    10441 ccatgatcac ttctttaccg cttttgatca attcgatcag ctgattgtac tgctccgcag
    10501 aaatagcaat ctgcggtgct accgatggcg gattaggatc ccacgctttg ggattcgtat
    10561 taccgaacgg atcggattcg tccacaagcg gcatggatgc acccataaca cgcactacgc
    10621 ccgaatgagg tgtaccgccg cgaaaactaa acgatcctcc gtccacgata agcgccggtt
    10681 gttgattatt gatccatgta aatatcctct gtctttcaat aaaagccgag aaacgaggcg
    10741 acttacgaaa actcctccga aaagattctg ctcgtccttc attttttccg gcattggaca
    10801 tggtcaataa ttcactatcg ctccagcgtt tagcatccgc ttcaaaatct tcctcgatct
    10861 ttaccttatc gcctaaaagt acaatggcat tcttgagttt gtttgaatat ttcttctgaa
    10921 tgtcttcgag tttcgtttcg ttgatataca agatttttga tcgaaccgtg ccgcgcgtag
    10981 cgggtgacca cgctttggga taagcgatga gtggtatcgc atacggcgcc acaatgccgg
    11041 cacgaaaact tttcatatcc catccgcgac caaattctcc ccacggttcg acttctgccg
    11101 ataagcccat tttttcaagt gcctcacgcg tccaatcgtt agcggccgct aatccttttg
    11161 aattagttaa acgtgcaccg atcacatttg taagaacatt cagagtttgc agagcttgcg
    11221 agcgctctat cgcctcggtt tttatggctt cggcggcgac cgaatcgcct ttttccgctt
    11281 gtgcaaacag tgaaagacta cagcacaaaa ttacaatcaa catacgataa aaatgcatgt
    11341 tacctcacag gatagtttat gtttattgat ttttggattc agcagtcgtc agtatttcga
    11401 taatcgtttc atgtttttta gcgcgcgcta tggaaagcgc cgtacgccct tgcatatctt
    11461 tggctttcaa atcaacgcca cgattaacta ataatcgaac agcctccatt cgattatagt
    11521 taaccgcaag catcaatacg gtttccccgt tgggtgttcg cacttcaaga tttgctcctt
    11581 tgtctaataa agcctccatg atcggcgctt tacctttgat tgcagcttgc atcagcggtg
    11641 taaatgaaaa acgatccatc gcattaggat ccgctccgcg atcaagtaaa agacgtacaa
    11701 catccaagcg atcaagcaat acggcaaagg tcagtgcgct tttaccgtct ttgtcacgcc
    11761 catttacatc ggcgccggcg actatgagat cggacacaag ttcagtcggt gcataacgca
    11821 cgccgtacat caatgccgtt tgcccattat cggcaacggc cgtcggatca gcacccttgc
    11881 gtaacagcga acgaacaata ccgatatgcc cttcacgcga agcgatcatc aatggcgtag
    11941 ggtcattgga ctcgccttgc gaaaccgaat ccttgtgatt ggtaaatctc atgtttggat
    12001 tcattccggc ttgaagaaac atattggtca aaataagatc accttttcga atgcgcgaca
    12061 gaaattcctg atcgttgtat gcaattccaa ggcgcatcaa atcgcgttga gcgctgtctt
    12121 ttggctttga acaggaaacg atgactaaaa aacacatcgc aagcataact atcgtacgca
    12181 taataactac attcctttta accgccataa ttccaaccgg tatcccgtaa taaaataggt
    12241 ttaaaatcac gacgtacttt ggcattaatg atggaggcta ctatgatcga atgatcaccc
    12301 cgttcgatac gatcggttac cgtacattcg atatacgcct gcgcttcatc caatataggc
    12361 gcaccgcttt gatccgtatc aaacgaatat ccattgatca tttggccgtt tacttcaatg
    12421 tttttaaaaa attttccagc aattgatttt tgatcttctg ataaaatatt cagcgcaaaa
    12481 gaatcggttt ggcgtaccgc ctcattggcc caactgtctt tgcgtaaacc cagcatgatc
    12541 agaggcggct caaaggatgc ctggcttacc caagtcacgg ttgaggcggc atacgggctt
    12601 ccttggaaat catgctgcgc tttggctgtc aaaatgaaca taccgtattg aaaaaaacgt
    12661 aatgcttttt ttttatcttc gatgatcata ttaatgttta tatcgttttg attaatttgc
    12721 cgacagtttc tgttatgcgc ttgatcgtat agtctatgtc atccgatgtg ttcccgcttc
    12781 caagtccgaa tcgcaatgag gctttcgcca attctttaga aatgcccatg cgcaataaaa
    12841 catgcgaggg ttcagggacg gccgacgaac atgccgaccc ggtggacacg gcaatatctt
    12901 tgagcgccat gataagtccc tcgccatcaa tgtccggaat gctgacattg agattattcg
    12961 gaagccgacg ggaaaaattg tcatcttttt caaaatcagg accatttaac cgaacgcctt
    13021 ttattgtttg ttttaagccg ctccataggc gttccctaag tcctgcatag tgtttttggt
    13081 tttgttgtaa ttcatcaacg gaaaaacgcg ccgcttcgcc catgcccacg atacccggca
    13141 cattaagtgt accggaacgc tgcccgtttt catgacctcc cccgtgtaaa agggccgtga
    13201 gttgaatacg gggattacgt tttcgaatat acagtgcgcc aacacctttg ggaccataaa
    13261 acttgtgcgc tgaaagggat aataaatcaa cattaaggtt ttctacatgc accgggattt
    13321 tgccaagagc ttgtaccgca tcggtatgaa gaataatacc tttctcacgt gtaatagctc
    13381 caatagcagc catatcctga atcacgccgg tttcattatt ggccagcatg acactcacca
    13441 aaatcgtatc gcttcgtatg gcgttcaaaa ttttgatttg atccaatata ccgtcttctg
    13501 aaacctccaa tacggtgatt tcaaaaccgg cttctcgtaa atattcacac gtatctaata
    13561 cactggcgtg ttcggtagaa accgtgatga tatggcgccc tttgcgttga aacgtttcgg
    13621 ctatgccttt gattgcaaga ttattagctt ccgtcgcacc actcgtaaat actatttcat
    13681 ccttgcttgc cccgatagcc gtagcgatgc ggattcgcgc ttgttccacc gcttcttcgg
    13741 cgatccaacc gtattgatgc gttcgactgg cggcattgcc gtaatgctcg gtcaaatacg
    13801 gcatcatttt ttgcaaaacg cgtggatcga gcggcgtcgt cgcgtgatga tcaagataga
    13861 tcggtttttt catggcgacg caaagtagat aatcactatc ataaaatcaa ccgacatacc
    13921 gatgtaaaaa aacttgttaa aaaaaattag ctttcgtaag ataggctaaa ccaaaaggaa
    13981 attaatatga aaaaaattct gacgttttta ttgttcacgg gcgcaatttc cgtttacgcg
    14041 caggaagttt ccaaacaact cgagcgccta gatccgggta cggccacggc caacctagat
    14101 aacttcggta aaactcacag tgaacaaatc acggcagcac atcaagctcg tctgggcgaa
    14161 ctcatcaagg ccggtacgat cattattcgc caggacacta tgatcaccgc ggataaaccg
    14221 gttaaaatcg catttaactg gccggttgca agcggtcaaa acctcaaaga taaatccgtg
    14281 gtcaaacgtg acccgcaggt aatgacactc tatcccgcta aagatccgag caacaaagat
    14341 attccacaac gatggcaagt cacttacaaa gacggcacag tcaaagcaat acaataaatt
    14401 ttttgtttag ttatcaaata agtacccctt catcaaaacg gatgaagggg tttttagtgg
    14461 acctaactga ggatattttg aattagaaat ttgtctcaag ccattttgtt gcaaaagcga
    14521 cttcatcctc atgagcgggc atacggacaa atcgttcatt gaatttccat gcccctgttt
    14581 tgggtgaaac tgacgaataa acaatcatta tcgttttcca attcgcgtcg cgcttttgtt
    14641 tatccgtttt ttcgccaaat gtttgttttt tggccatatt ttttataact ccatataaaa
    14701 gttcatagat tagttcacta cgctctcttc cgcggattgg attatccact cgggaaacgg
    14761 atcaactaat ctcgtccaac cggtatggcc gattttcatc tcttcatcgg taagcaaaca
    14821 cttttcaaat gattggctta acgccgcttt atccatgtct ttacctatga ggacgatttc
    14881 ctgacggcga tctccgtatg gcggttgcat ttgtgcttcg atatcttgta tatacgccgc
    14941 ttcgtccggc catgaacggc gatctatcgc tgaccaccaa aaaccggccg gttcatagtt
    15001 gcatgtgccg ccagcttgtt cccagtgccc cgcccactcc atgcgtgtcg ccagccaaaa
    15061 ataacccttg gatcgaagca ctccgggcca ctctgtatgc acccacgccc aaaatcgttc
    15121 gggatgaaat ggctttctcg cacgaaaaac aaaactggaa atgccgtatt cctccgtctc
    15181 cggcgtatgt tcaccgcgca attccttaag ccaaccggga gccgacgaag ctttatcaaa
    15241 atcaaaaagt cccgtatcca atacttgcgc caatggcaca tcgccgtatt tagaaaaaac
    15301 aatctgcgcg tcaggattca atctttggca tacggaaaac aacacttttc tggcctcttc
    15361 accgatcaga tctgctttgt ttacgattat gacattggca aactcaattt ggtctatcaa
    15421 caaatcggcg atcgaacgct catcttcctc acccaaagca atgtttcgat ctatcagtga
    15481 atcgctgccg caatagtcat taaaaaaatt atacccgtcc acaacggtaa ccatcgtatc
    15541 taatctggcg tagcgcgata aactttcgcc ctgttcatct tcaaacgtaa atgtttcggc
    15601 aaccggcaac ggttcggata tgccggttga ttcgatcaag agatagtcaa atcgtccttc
    15661 tttggccagt cgcgctactt cgaccagaag gtcttcgcgc aatgtacaac aaatgcatcc
    15721 gttggtcatt tcaatcaatt tttcttcttt gcgacttaag gccgcttcgc catttttgac
    15781 gagcgatgca tcaatattta cttcggacat atcattgacg attaccgcaa cacgtttacc
    15841 ttcgcggttt ttcaaaatgt gattgagtaa tgtcgttttt ccggcgccta aaaaaccgga
    15901 aagcacggtg acaggaagtt ttttagtcat aaaagcatcc ctaaaataag tgtaaatgaa
    15961 gtacaaatca tcacaggccg gagttcggca atacataata ccgatctttt gcgtaaggcc
    16021 aatgacaaag gaataatgga aaacctccgt tagaggtttc gttatttaag gaataaatgt
    16081 gaatgtgtat ttagacggac aatggcggag ctcgagcaga acgacgaggt aaaatacttt
    16141 caagaaacca aacatgactg tggcctttgt tctcattgat aatagacgca ataacgagtg
    16201 gcaacgaatc aacatgatcg tgccccgatg caaattgatg agccaaacaa tacgaatgtt
    16261 gggcgccttc ccctgtagaa taaccgagat cgcggacggt aatttcaacc gtatgaaccg
    16321 atatgccgca ttgatccgta tgcagcctcg gtagaatgct gcaaacgatc aggtaaaaaa
    16381 caatgtatga atatcgtaac aaagagtgca cgtttccgct ttccgatgag aactaataaa
    16441 ggcgtcacat ggtaagtaac gccttgtatt tataagaaac cttattgaat acgaatcgga
    16501 aactccacct caatatccgt ttcgccgggt gtagatgtac cgtttttgac tccgggctgg
    16561 tgtttaagcg tcacatgcgc ttcgccactt gaggccgcac cggtaatcca acgcgtcaat
    16621 aaaccgacgg gaatattatt atcatccaca tcgcgatagt ttacaacaag gttaacatcg
    16681 tggccatgaa agaaaaacat atgatcatta cgttcatcat acacttcatt cgaaaccgta
    16741 tcggcgggtt cttccgtttc gtttaataaa acgatcgtag cgtgataggt tttgtttgcg
    16801 gctaatttaa ttgtatcaaa ctgtaccgga tccgcgccgc cttcaccgtc caaatcgcga
    16861 aacgaggcgg taaccgtatt cgccgcattc gacgtatcta caaacttaat caccaccgat
    16921 gtgatgagtt cttccggatt ggtttcctta tcgtcttttt cgcatccgct gaatacaaat
    16981 aatgacatca gtatagttac agttaataac gccgtttttg agaaaactcg tgtcatacaa
    17041 cactccttaa gtttttcgat taatgattaa ttttaactaa aattctaggt tgccaaacgg
    17101 aatctgaaca cgcagaacaa tattgcgacc gggatcgtct atataatatc gaaaccggct
    17161 cagataattc gcataccgcg tattgaacac attattgatg gccagactga tcgtaatcgg
    17221 cgaccaggat gtatgccatt cgcaccctac ttcaaaatca accgtcacat aaccgggtgg
    17281 cggagtgacc gtaacggagt cggcatcgtt tacaaaggtc tcagcgcctt caggtacatg
    17341 cgattggcga cgataccatg ttatcccgat atccacgtac ggttcatgga tcgtgcctgc
    17401 atctgaaaaa tgccaatgca taagcccact taatcggtcg gaaggcatat aaatcaaggg
    17461 ctcgtctttt ttcaaatcgc ggccgcgcac gatagaccat ccggcatcaa gacgcacgat
    17521 gtcaagggca tgccattgga tctggccgtc tataccggtc aatcgagctt ccgtttgcgc
    17581 ataacgaaac gtcggaaaag atcccctgat cgtgagcgta ggtttacgcg atggatacaa
    17641 atgaatatag ttatccatgc ggtttacaaa caaactcaca tcgccgttga tcttttcgct
    17701 gttatgatgc aaagtgatat ccgtactata gcttttttct acgcccatcg cgggatcgcc
    17761 gatttcgtat tgtgccgtgc catgatgaac accgttacta tataactcat ttactcccgg
    17821 cggtcgccac gccgttccgg cattgatccc gattgaccag tattctgcaa aacgtcggtt
    17881 cacgccgacg gccgccgatg tactgcgata aacatgatct tctttttcat ttacacccaa
    17941 cttcaactgt tgttgcgctg taaaggaagc atccagccat cgctgatcgt agcgcaaccc
    18001 cgcctcggct gaccaatcat cctgcaccca tttttcgatc caatacacac cggcattata
    18061 agtgtgaaaa ctgggaatca gacgtatcgc ttgattgaca ttacgctgcc gcatgacatt
    18121 gacgccgatg gttccggcaa agtccccgat cggacgatga cgccatcgca catcggctgt
    18181 gtaggtgtac agcgaaagat caaaggaagg tttttttaaa tcctcggccg gtgtaccgag
    18241 tggcttgtgc gaatcaaact cctgacgatg attgcgttgc cagccaccct gaaattcgaa
    18301 acgatcgccc gcatccgttt gacggtgtac acgagccgat atcagatcat gagtgattac
    18361 ctgacgggga tttccgatcg cgaaggaaaa atcatcctcg tttaatggaa cgcggttgcg
    18421 tttgatcgca gcgataagat cgctggtatt tccgatatgc gaacctttgt agataccaag
    18481 ttccgttcca aaatgactat aatacaactg cgattcccag cgatggtttc gaaatccgac
    18541 cgcggcagcc ccgttgaact cctgaaatcc ggaattgccg atgacataac gcggtgtttg
    18601 agcattaccc gccttacgcc cgctgacctg tgcacgccaa ccgaaaccgg gtttattggc
    18661 aaggccgcct tcgagtcgca atgctccggc catttggcgg ttattcgtaa aaccgttgag
//
LOCUS       DAOQGF010000062        18626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1385_length_18626_cov_6.294007, whole genome shotgun sequence.
ACCESSION   DAOQGF010000062 DAOQGF010000000
VERSION     DAOQGF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1385_length_18626_cov_6.294007"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1902
                     /locus_tag="PK937_09630"
     CDS             <1..1902
                     /locus_tag="PK937_09630"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HNH32871.1"
                     /translation="SSRYYQNNNDGTFTRIDTLAMSNGTNPRRGATAGDFDQDGDLDL
                     YVYSSFTGVNNFFRNDLTNGNHWINFRLSGKVTNHTAWGAIIRVKATIGGNSRWQTRQ
                     MSAQNSFSGHSAELQHFGMADATNVDSVEIRWPSGIRQYVTSLAVNQTVDIIEDTSLS
                     YKPNFKWLSNNTTFVDSSYTTTLFAHANPKAVYRLIQSPSGMTIDSISGLIQWMPNAT
                     QTGNQTVRVSAVNSQGTQERQYTLNVIQLVKPQIIPLPDRATMVGMNVSVFAKYVTTP
                     TQPATYQLVQGPAGMTINSIAGHITWKPTSSQLGSHTIKIRSVNSVGSDSITFTIDVK
                     EQPKIYVLQNPVATHTVDLITISEISLRSKPFIQVTQRVFAPGQGPFVVSDSVSPGVY
                     RGSWVFDSTRTYQINATFSDSNGFTGSLQKIFTVAIIAADKPTLIQTMDSRFNVFVPA
                     RAIASERFVIIESSQEAGMDFINFGNALELQSQAHIELNAWRNPKIEFETINGWEEIP
                     VERVGRLYRANIKHLGKFRISESTDSGISDKVPHNLSLSQNYPNPFNPTTTIRFDLHQ
                     TSDIRLTVYNTLGQRIRLVTQGVFSAGTYRVIWDGRNDQGAAVASGIYIYRLESDGNF
                     FSKRMMLIK"
     gene            1918..2505
                     /locus_tag="PK937_09635"
     CDS             1918..2505
                     /locus_tag="PK937_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32872.1"
                     /translation="MKKLTYIFLTGVFYLLGCGGGRDSADPIQKGWSAFESGEFYESR
                     DIFLNIYLDNPNDAEAAHGLSWSLLRMDSVSRAVTMFESSEGALPPEDVTGGYTFALS
                     AKKMYNESNTQADILINASPTWTFTHGSEINVIDIYIVKSANYFALGNYPASLNSIKM
                     VNASFSADVTTASGRSSLAAEIERLRGLNKVNISE"
     gene            complement(2587..3966)
                     /locus_tag="PK937_09640"
     CDS             complement(2587..3966)
                     /locus_tag="PK937_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH32873.1"
                     /translation="MKSAKYILILAAMFISVAFAQKETPPKGSAPKDFSLPKRETISL
                     PNGLQASLIRYGNVPKAMIRVVVRTGNIDEKNNETWLADLTGEFLSEGTASKNAEAVA
                     TAAANMGGSLNINTGLDQSFVAGEVLSEFTPDFIKLSADVIRNPAFPASELERLKSKM
                     LRDLSVQSSTPQSQALEKYSKVMYGDHAYGRTFPTEAMLKSYTLDQVKSFYVANFSAK
                     RTHIFVVGVFDPAAVTKAIREAFGDWKEGTPPTQNVPQAKSERTIYLIDRPGAPQSTI
                     ILGLPTMDASHPDYVKFLIMNSILGGSFGSRITTNIRENKGYTYSPFSAITSRYHSAN
                     WSEQADVTTEHTGASIKEILYEIERLKKEPPAKDELEGIQNYQAGIFVLQNSNFNGIV
                     NQLAFLNLHGLPDTYLSNYVKNVYAVTPEDVSNMAKSQLKDDAYTIVIVGDIAKIEKQ
                     VKLYGKIVK"
     gene            complement(3963..5360)
                     /locus_tag="PK937_09645"
     CDS             complement(3963..5360)
                     /locus_tag="PK937_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH32874.1"
                     /translation="MKPGLRLLSLGLSILLVVSSCAKKEDAVPKFSVPVEYHKLENGL
                     KVVLSPDATAPTATVAVYYNIGFRIEPKDRTGFAHLFEHMMFQGSQNLGKMEFIQLIQ
                     RNGGILNGSTRFDFTNYFQVVPSNKLETILWGEADRMKGLAITKENLKNQQEVVKNEV
                     KVNVLNQPYGGFPWLDMPQYANTNWYNAHNFYGDLKDLDAATLEDVQSFFKTYYAPNN
                     AALVVVGDFEPKQTLEWIKKYFGSIAAQPQPAKPDISEPEQTGEKKSKKQDPLATRPA
                     VAVAYQMPARNTPEYYAMGLLDQILVQGEDSKLHQALVQKKGYTGQVSGGINYLGNMF
                     NYNGPMLWMMDLIHDQNVSADSIMLEVDRIMQELAEKPVDQATLNRALVKMRSSLYGE
                     FNQTGGLGKMDLLACFALFDDNPARINTLEDEFRKVTPELIRETAKKYLRSTNRTILS
                     VEATNVMKNKMGAAQ"
     gene            5582..6664
                     /locus_tag="PK937_09650"
     CDS             5582..6664
                     /locus_tag="PK937_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNH32875.1"
                     /translation="MQKETGILDTLSNDVEKADFWHTMKREYRELHEFYIDSDRKKRL
                     LSMGRFKRVMYIAAWILQGMFYKLTPARRLLIVAGILLSINGITFKFASWLVGDRGLL
                     GALLLFFVLMLELKDKLLAHDELDAGRTIQRALLPPEQPNIPGWSVWLFTRPAREVGG
                     DLVDIIDHDSKTMSILMADVSGKGLRAALWTAKLQATIRALITMITDPLDLVDRVNKI
                     FHRDSLRSVFASMVLLRVNKSSGQIQYVNAGHLPPVLCNRDGKTECGRGQLAIGLSPS
                     SKYVLNELTMAIGDVLVSFSDGVTEAANENGEFYGNDKPLQIAERLRNTDAPVIGKAI
                     LLDLERFVGESSASDDISIIILKRHS"
     gene            complement(6680..7108)
                     /locus_tag="PK937_09655"
     CDS             complement(6680..7108)
                     /locus_tag="PK937_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32876.1"
                     /translation="MNKTINIFIIVVLHTAAHVLVAQNDEDLVSFIIGQYEIIGRYPG
                     SKKPFIGSAVVTLDNGKIFVKRTIDKKTEMADAEFIKVTADQITVLQCRFQSGKETYV
                     GVYMIKGDLDNYPRLTGQLFKSKDESFPSGIEAWFPIIPN"
     gene            7314..8039
                     /locus_tag="PK937_09660"
     CDS             7314..8039
                     /locus_tag="PK937_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH32877.1"
                     /translation="MEKVMQSDRGFKSVLAEVYQKNMVKGLSAGVVLHAFIIGTIVWV
                     IGQNPKAVKQVMNQISILVDMPPLTNIPEIPAINLPQKFNPSDIIKGIPIIAPDAIVS
                     PDITIPAQDDWIEKFSDNSVPMDWKNLDVSLPNFENVSIDSKNTSSGEFTIVEQMPEV
                     IHRVKPEYPSLPRQAGIEGRVYVKGLVNEEGMVTRTVFVSGDEMFYNAASDAFLKTRF
                     KPGINGGVAVKVWIMMPFTFRLR"
     gene            complement(8084..10714)
                     /gene="clpB"
                     /locus_tag="PK937_09665"
     CDS             complement(8084..10714)
                     /gene="clpB"
                     /locus_tag="PK937_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNH32878.1"
                     /translation="MNSDKMTIKAQEALSKAQQLALERHHTQIEPEHILMAMLDDHDG
                     TVPALIKKLGSPLATIRTQAESILDRMPRVSDTAQQVYLSGRASRILIEAGKEAEKLK
                     DEYISTEHLLIAMAEESTESGKMLKGFGIWRETLYRALRDVRGAQRVTDQNPEGKYQA
                     LQKYGRNLNELARRSKLDPVIGRDEEIRRVLQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAHRIIDGDVPENLRTKTVMALDMGALIAGAKFRGEFEERLKAVLREVTESQGEIILF
                     IDELHTLVGAGAAEGAVDAANMLKPALARGELRAIGATTLDEYRKHIEKDPALERRFQ
                     PVLVDEPSVHDTISILRGLKEKYEVHHGVRIQDNAIIAAAVLSDRYIADRFMPDKAID
                     LIDEAASKLRIEINSMPEELDTVERTIKQLEIEQLALKKENDTVSLSRLDKISSELAD
                     LKEKRTALRSHWETEKKVIEQIRRVQSEIEITKSQIEQSERAGDLNKAAELKYGRLTE
                     LGTQMEKEKNKLAEVQKNQKMLKEEVSEDDIAEIVAKWTGIPVSRMLESEKDKILSMP
                     ERLQERVVGQNEAVVSVSDAIKRSRAGLSDEKRPIGSFIFLGPTGVGKTELAKAVAEF
                     LFDDETAMVRLDMSEYMEQHSVSRLIGAPPGYIGYDEGGQLTEAIRKKPYSVILLDEI
                     EKAHPEVFNTLLQVLDDGHLTDNKGRTVNFRNTIIIMTSNLGASVIMEKMNDMASENR
                     DRIHARIKESVISLLRQRLRPEFINRIDDIVVFRPLERNDLQRIVHIQFAHVKKLLQK
                     HNIGIELDTTAVDALARLGFDPIFGARPLKRLIQKDVVTQISTELLKGAFVPNDQLLL
                     AFHDDAFRLTKRNNSLKSVA"
     gene            complement(10788..10937)
                     /locus_tag="PK937_09670"
     CDS             complement(10788..10937)
                     /locus_tag="PK937_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32879.1"
                     /translation="MHVVRSITCGGMRVPTIPHLFTEQNAMAYGLKISVILMICLALY
                     MIMTA"
     gene            complement(10956..11405)
                     /locus_tag="PK937_09675"
     CDS             complement(10956..11405)
                     /locus_tag="PK937_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH32880.1"
                     /translation="MSLIKFTPRDRDIFSDLFAIQREMNQAFNSVFNRDEYTGLTNWN
                     PATDIVEGKDEYTVRIELPGVSKNDVKITIHDNVLTVQGEKKQESENKENNYHRVERS
                     YGSFMRSFRLPSMVKGEKIDANYKDGILTIVLPKAEEAKTKEIEIKF"
     gene            complement(11433..11819)
                     /locus_tag="PK937_09680"
     CDS             complement(11433..11819)
                     /locus_tag="PK937_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNH32881.1"
                     /translation="METKQEYEVKTQAIAKPENNYYYIQPRTDIRENKDNYLLYVEMP
                     GVRKDGLTVTLQNGNLVIEGKMGLNEEGSVIREEIPKRNYHRVFRLTNRVDPNKIEAR
                     WQDGFLILSVGKKEELKPREIAINFN"
     gene            12229..13014
                     /locus_tag="PK937_09685"
     CDS             12229..13014
                     /locus_tag="PK937_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNH32882.1"
                     /translation="MNEEFQIRLDIVECGRRMYARNYVASNDGNISVRVGDRIIATPT
                     GISKGFMRAEDMVVLDIQGRVIEGARKPSTEIKMHLAIYEERKDVNSVVHAHPVHATG
                     FATAQIELKDCVLAEIVTTLGSIPLAPYATPSTTELPDSIRPIIRRADACLLANHGVV
                     TCGRDVYDAYYKLERVEHYARILFVAKSLGGAKILSPQQVHKLETIRATYGTSDAHNP
                     GCIACHGACIGSDCTLYETQQRDQSASDLHVEETVRAIISAKK"
     gene            13015..13806
                     /locus_tag="PK937_09690"
     CDS             13015..13806
                     /locus_tag="PK937_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH32883.1"
                     /translation="MNTEFGNRSPLRSLLTIIAAVVFTFLMIIAATIAALFGPRSKAT
                     HAVEKFWARTILRMAGVRARVRGLEHIRPDHVYIFISNHQGLFDIPLMMAYTPKQLRM
                     IHKKELLWVPFFGFILWLLDFIAIDRGNRQRAAESLHRAAKKIHDGTNIVIYADGTRS
                     IDGELRPFKKGAFILAIDAQVDVVPVTISGTINVIHKFEGVFDIRFGQTVELVFSPPI
                     STDGMMPEAKDDLKDQVQNIIAAEYSKIKHLSAITNPAILSRLKK"
     gene            13926..15074
                     /locus_tag="PK937_09695"
     CDS             13926..15074
                     /locus_tag="PK937_09695"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNH32884.1"
                     /translation="MKRIGLFGSTGSIGENVLQVIRNHPDRFTVQCLTANTNAELLIK
                     QALEFKPQSVAIVSDEGYAQLHSGLSGTGIRIYHGHDAIVQMARDENYDLMIGAIVGF
                     AGFLPTIEAIRAGKDIGLANKETLVVGGAIVNGLLSKHQSRLIPIDSEHSALFQCLVG
                     EDMRSVRKLILTASGGPFLHTPAEDFSSITVARALKHPNWSMGAKITIDSATLMNKGL
                     EVIEAHWLFGVGADRIDVAIHPQSIIHSMVEFVDGSTKAQLGVPDMKVPIQYAMTYPE
                     RMTNNFDVFDFRKWNRLDFYEPDRNKFRCLKLAYDALETGGSMPAVLNAANEIAVAGF
                     LQEKIRFTDIASSIESAMQAHTCVCDPDIASVIETDRWARAYVQKTCL"
     gene            15103..16617
                     /gene="rseP"
                     /locus_tag="PK937_09700"
     CDS             15103..16617
                     /gene="rseP"
                     /locus_tag="PK937_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNH32885.1"
                     /translation="MSIIFDALIPFLIVLGILVFIHELGHHLAAKLFGMKVDAFSLGF
                     PPRAWGFHWATKKLRPKFIKDITQSILSNTTFQSLDKEIQAAGLAQSSDASVKFLDTI
                     FEITQVEEKIELIDAKGKKEEKINLRTELRFAESGPAASLTNLQRDAWLVRVQGDADL
                     LDDLLKYGEINDDKTFHEKYKTDYCLSWVPLGGYCKINGMVDESLDEDSMKEGTPKPW
                     EYRAKPIWQRMIVITGGVFFNFLLAAFVFTLMGLVRGLPDMEKFDNWRRENALPAEVG
                     VVSPGSPADKAGIQAGDRIASINGQTVQKWDELVDVIRHNGGKKLPIEWTRGSERFSG
                     EVMPTIDKIQTEKGIEEIGRIGIQAPPFPEFTRDASFIEAVQHGFITAVGATYFIVRS
                     IKQMVSGEVNAKDALGGPLMIAKTTGDMKRQYGWEGIWNLMALLSLSLAVFNLLPVPA
                     LDGGHLFMLAIEGVMRRPLSLKVKLRAQQAGMAILLSLIVYIFFNDIVKLTGIF"
     gene            16614..17825
                     /gene="tgt"
                     /locus_tag="PK937_09705"
     CDS             16614..17825
                     /gene="tgt"
                     /locus_tag="PK937_09705"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNH32886.1"
                     /translation="MKFTLLHKDQSGRHSRARAGILETDHGKIETPIFMPVGTVGDVK
                     AIEHRELKENGAQIILGNTYHLYLRPGTEIIQKAGGLHKFIGWDLPILTDSGGYQVFS
                     LSQLRKLSRDGVKFQSHIDGSKHFFRPEDVVDIQRKLGSDICMVLDECPPYPCSHEYA
                     ARSNRLTIEWAERCKTHFEKSPPLYGHSQTLFPIVQGSVFEDIRLKSAEALVKMDFEG
                     YAIGGVSVGEPKEDVYKIAEMNCAVLPEHKPRYLMGVGTPQDILECIERGVDMFDCVM
                     PTRNARNGTLFTTWGKMTIRNAAYADDFTPIDENCGCYTCRNFTKAYVRHLSSIGEIL
                     GHQLTTIHNIYFYLWLVKEARKHILQDTYTAFKTDLLKRYEENEKNRGSELARHAKTK
                     RKRKDYETSGA"
     gene            17806..18132
                     /locus_tag="PK937_09710"
     CDS             17806..18132
                     /locus_tag="PK937_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH32887.1"
                     /translation="MKLVAPDALKAWIDEGKVMMLLDVREPWEHAIAKLPDSVLIPLG
                     LLNVQSERLTGLDKTTPVVAYCHHGMRSQRACAILESLGFTDVYNLTGGIDYYSDCVD
                     PTIEKY"
BASE COUNT         4984 a         3884 c         4319 g         5439 t
ORIGIN      
        1 agcagccgat attatcaaaa taataacgac gggactttta cccgcattga tacactggca
       61 atgtccaacg gaaccaatcc acgacgcggc gctacagccg gagattttga tcaagacggg
      121 gatttagatt tgtatgttta tagttctttt acgggggtta acaatttctt tcgcaatgat
      181 ctgacgaatg gaaatcattg gattaatttt cgcttatccg gcaaagtaac caaccataca
      241 gcttggggcg ccattattcg cgtcaaagca acaatcggag gcaattctcg atggcaaacc
      301 cgccaaatgt cggcacaaaa ttcgttcagc ggtcatagtg cggaattaca gcatttcggt
      361 atggctgatg ctacgaatgt tgattcggtt gaaatacgtt ggccatccgg gatacggcaa
      421 tacgttacct cattagctgt taaccaaacg gtagatatca tcgaagacac gagtttgagt
      481 tataaaccta attttaaatg gctatccaac aatactacat ttgttgattc atcatataca
      541 accacgttat ttgctcatgc caatccaaaa gccgtttata ggctcattca gtcaccctca
      601 ggtatgacga ttgattccat tagcggttta atacagtgga tgcccaatgc tacacaaacc
      661 ggtaatcaaa cggtgcgtgt atcggctgtt aattctcagg gtactcagga aaggcaatac
      721 acgcttaatg taatccagtt ggtaaaacct caaatcattc cactgcctga tcgtgcgacg
      781 atggtgggaa tgaatgtaag tgtttttgca aaatatgtta ccacacctac ccaaccggct
      841 acctatcaac tggtacaagg gccagcgggg atgactatca atagcattgc aggacatatt
      901 acatggaaac cgacttcgtc gcaactcgga tcgcatacta taaaaatcag atcggttaat
      961 tcagtcggtt ctgattcgat aacatttact atcgatgtca aagagcaacc gaaaatttac
     1021 gttctacaaa acccggttgc tacccatacc gttgatttga ttactatcag tgaaatcagc
     1081 ttacgatcaa aaccgtttat acaggttacg caaagggttt tcgcacccgg gcaaggtccc
     1141 tttgttgtat ccgactcagt gtcaccgggt gtttatcgcg gatcatgggt tttcgattcg
     1201 acacggacat accaaatcaa tgcgacattt tcagattcca atggtttcac aggaagtctt
     1261 caaaaaatat tcacagtagc gatcatcgcc gcagataaac ctacattgat tcaaacgatg
     1321 gatagccggt ttaacgtctt tgtcccggcg cgcgccatag cctctgaacg attcgttatt
     1381 atcgaatcgt cacaagaagc gggtatggat tttataaatt ttggaaatgc tctcgaattg
     1441 caatcacagg cacacataga gttgaatgca tggcgcaatc ctaaaataga atttgaaaca
     1501 attaatggtt gggaagagat tccggtcgaa agagtgggtc gtttgtatag agctaatatc
     1561 aaacatctag gaaaatttcg aatttcggaa tctacagact ctggaatttc tgacaaagta
     1621 ccacataatt tgagtttatc tcaaaattat ccgaatccat ttaatcctac gacgacaata
     1681 cgatttgacc ttcatcagac gtcagatata cgattgacgg tttataatac gcttgggcag
     1741 agaatacgtt tggtgacaca aggtgttttt tccgccggta cgtatcgtgt tatatgggac
     1801 gggcgcaatg atcaaggtgc agccgttgca agcggtatct atatttatcg tctcgaaagc
     1861 gatggtaatt ttttttccaa acgaatgatg ctaatcaaat aaactaaggt ataatctatg
     1921 aaaaagttga catatatatt tttaacagga gtattttatt tattgggatg cggcggcgga
     1981 cgtgattctg ccgatcccat tcaaaagggt tggtcggctt ttgagtcagg cgaattttat
     2041 gaatcacgcg atattttttt gaatatttat ttggataatc ccaacgatgc ggaagcagcg
     2101 cacggtttga gttggtctct tttgcgtatg gattcggtta gccgagctgt taccatgttt
     2161 gagtcatcag aaggagcatt gcctccagaa gatgtgacgg gcggttatac atttgcactg
     2221 agcgcaaaaa agatgtataa cgaatccaat acacaagcgg atatattgat caacgcctct
     2281 ccgacgtgga cgtttaccca tgggtccgag attaatgtta tagatatcta catagttaaa
     2341 tcggctaatt attttgcgct tggtaattat ccggcctcgt taaacagcat aaaaatggtc
     2401 aatgcgtctt ttagcgctga tgtaactacg gcttcaggtc gctcttcact ggccgcagaa
     2461 attgagcgat tacgaggatt gaataaagta aatattagtg aataagttga ttgaattcag
     2521 ttttcaagtt tagaaataga aaaagcacgc cgcaagcgtg ctttttctat aggataatac
     2581 aacgtattat ttcacgatct ttccgtacag ttttacctgt ttttcgattt tagcaatgtc
     2641 tccgacaatg acgatcgtat aagcatcgtc ctttaattga gacttagcca tgttggatac
     2701 atcctccggt gtcacggcat atacattttt aacataatta ctaagatacg tatccggtaa
     2761 accgtgcaaa ttgaggaaag ctaattgatt gacgataccg ttgaagtttg aattttgtaa
     2821 tacgaaaatc ccggcctgat aattttgaat tccttccaat tcatctttag ccggaggttc
     2881 ttttttaagg cgctcgattt cgtataaaat ttctttgata gaggcaccgg tatgctcggt
     2941 tgtcacgtca gcctgttccg accagttagc gctatgatat cgtgaagtga tagcgctgaa
     3001 aggcgaatac gtgtagcctt tgttttcgcg gatattagtg gtaatacgcg aaccgaatga
     3061 tcctcccaaa atggagttca ttatgagaaa tttgacataa tccggatggg aagcatccat
     3121 cgtaggtaaa ccgagtatta tcgtagattg cggtgcgccg gggcggtcaa tcagataaat
     3181 tgttcgttcg gacttagcct gaggtacgtt ttgtgtcggc ggggtccctt ctttccagtc
     3241 gccaaaggct tcacgtatgg ctttagttac agcagcaggg tcaaatacgc ccacaacaaa
     3301 aatatgagtg cgtttagctg aaaaattggc cacataaaat gattttacct gatcaagggt
     3361 ataactcttg agcatggctt cggtaggaaa agtacgtcca tacgcgtgat cgccatacat
     3421 cactttgctg tatttttcca gtgcttggga ttgcggtgta ctgctttgta cgctcagatc
     3481 acgaagcatt ttggatttta gacgctcaag ttcagaagca gggaaagcgg gatttcgaat
     3541 cacgtcagcc gaaagtttga tgaaatccgg agtgaattcc gataagactt caccggctac
     3601 aaatgattgg tcaagcccgg tattgatatt taatgatccg cccatattgg ctgcagctgt
     3661 tgccacggct tcggcatttt ttgatgctgt gccttcactc aaaaactcac cggtcaaatc
     3721 agcaagccac gtttcgttgt ttttttcatc aatattaccg gtacgcacaa cgacgcgtat
     3781 catggctttg ggtacattgc cgtagcgaat gagcgaagct tgtaaaccat tgggcagaga
     3841 aatagtttca cgttttggca gtgaaaagtc ttttggcgct gaacctttag ggggcgtttc
     3901 tttttgggca aaggccacag aaataaacat ggcggccagt attagaatat attttgcgga
     3961 tttcattgcg cggctcccat tttatttttc atcacattgg ttgcttctac cgaaagtatc
     4021 gtccgattgg ttgaacgcag atatttcttg gctgtttcgc gaataagctc cggcgtgact
     4081 ttacggaact cgtcttccag cgtattgatt cgtgccgggt tatcatcaaa taaggcaaag
     4141 caggcaagta ggtccatttt gcccaatccg ccggtttgat taaattcgcc atacagcgat
     4201 gaacgcattt tgactaaggc acggttgagt gtagcctgat caacaggttt ttctgcgagt
     4261 tcctgcataa tacgatcaac ttcgagcatg atgctgtcgg ccgaaacatt ctgatcgtga
     4321 ataagatcca tcatccaaag catcggcccg ttatagttaa acatattacc aagatagttt
     4381 ataccaccgc ttacctggcc cgtatatcct tttttctgaa ctaaagcttg gtgtaatttg
     4441 ctgtcttcgc cctgcacaag gatttgatcc aacaatccca tggcataata ctcaggcgta
     4501 ttgcgagcag gcatctggta ggccacggct actgccggac gagtcgcaag tggatcctgt
     4561 tttttggatt ttttctctcc agtttgctcc ggttcgctga tatccggttt cgccggctgg
     4621 ggttgagccg cgatactgcc aaaatatttc tttatccatt ccaacgtttg tttgggttca
     4681 aaatcaccga ccaccaccaa cgcggcattg ttgggtgcat aatatgtttt gaaaaatgac
     4741 tgaacatctt caagcgtagc ggcatccaga tctttaaggt cgccgtaaaa attatgtgca
     4801 ttataccaat tggtattggc atactgtggc atatcaagcc aagggaatcc gccataaggc
     4861 tgattgagaa cattgacttt tacttcgttt ttgacaactt cctgttggtt tttaagattt
     4921 tctttggtaa tagcgaggcc tttcatacgg tcggcttcgc cccacagaat ggtttccaat
     4981 ttattagacg gcacaacttg aaaataatta gtaaaatcaa aacgggtcga gccattaaga
     5041 ataccgccgt tacgttgaat caattggata aattccattt taccgagatt ctgtgaacct
     5101 tggaacatca tatgttcgaa taggtgtgcg aatcccgttc gatctttggg ttcgatgcga
     5161 aagccgatat tgtaatacac ggcaacagtc gccgtcgggg ctgtggcatc cggtgaaagt
     5221 acgactttca aaccgttttc aagtttgtgg tattctaccg gaacactaaa tttgggaaca
     5281 gcatcttctt ttttggcgca tgatgatacc accaacagaa tgcttagtcc caggcttaac
     5341 aaacgcaatc ctggtttcat cggaactcct ccatgggttt atgagatcgg aatagtttca
     5401 aaagtaaatt attactttgt cagaaatgaa tcgtaacaag gctaattcgt aatcggttac
     5461 ggataaatta taaataagtt tcaatttatg ttagtataat tattccgttt acttgttggt
     5521 gtgatttaca aacatttgcg ggttatcttc gtataaaaaa aataataaca gattgaagat
     5581 tatgcaaaaa gaaacgggta tactggatac gttgagtaac gatgtcgaaa aggctgattt
     5641 ttggcatacg atgaaacggg aataccgcga actgcacgag ttttacattg actcggatcg
     5701 aaaaaagcgg ttgttatcca tgggtcgctt caaacgtgtc atgtacatcg cggcgtggat
     5761 attgcaaggt atgttttaca aacttactcc cgcccgacga ttattgatcg tagccgggat
     5821 tttgctgagt atcaatggga ttacatttaa gtttgcatca tggcttgtgg gagatcgggg
     5881 gcttttgggt gcgttacttc tcttttttgt cctcatgttg gaattaaagg ataaactgct
     5941 ggcgcatgac gaactggatg ccgggcgaac gattcagcgg gcgcttttac ctccggaaca
     6001 gcctaatata cccgggtggt ccgtttggtt atttaccagg ccggcacgcg aagtaggtgg
     6061 agatttggtg gatattatag atcatgacag caagacaatg agtattctga tggccgatgt
     6121 ttcaggaaag ggattgcgtg ctgctttgtg gacggccaaa ttgcaagcca cgattcgtgc
     6181 actgatcact atgataaccg atcctttgga tttggtggat cgggttaata agatatttca
     6241 tcgtgacagc ctgcgtagtg tatttgcctc catggtgctt ttgcgtgtca ataaatcttc
     6301 ggggcagata caatatgtca atgccggaca tttaccccct gttttgtgca atcgtgatgg
     6361 gaaaacggag tgcggacgcg ggcaattggc gatcggactt tcaccgtcat caaaatacgt
     6421 tttaaacgag ctaacgatgg cgattgggga tgtgctcgtc tcgttttcgg acggtgtgac
     6481 cgaagcggct aatgaaaatg gagaatttta tggaaatgat aaaccgctac aaatcgccga
     6541 gcgattaaga aatacggatg caccggttat cgggaaagcc attttgcttg atctggaacg
     6601 ctttgtgggt gaatcttccg cttccgatga tatttcgata attattttaa aacgtcacag
     6661 ttgaaaagat aataacgaac taatttggta tgatcgggaa ccacgcttca atacctgaag
     6721 gaaatgactc gtccttgctt ttaaaaagct gtccggttag ccgtgggtag ttgtccaaat
     6781 cacctttgat catataaacc ccgacatatg tttcttttcc cgattggaac cggcattgga
     6841 gaacggttat ttggtcggct gtcaccttta taaattcggc gtcagccatt tcagtttttt
     6901 tatctatcgt acgttttaca aatatttttc cgttatccaa agtcaccaca gccgaaccga
     6961 taaatggttt tttactgccg ggatatcgac cgataatctc atattgacca attataaaag
     7021 aaacgagatc ttcatcattc tgtgcaacca aaacatgagc tgcggtatgc aaaacaacga
     7081 taataaagat atttatagtt ttattcataa aaccctttcg atgtgtatat ttaatccatg
     7141 aatagccaaa attaaatcgc aattattgta ataacaacat attcagacag ttcttaccga
     7201 ttatcaccat tctattgttg gtgatatgga gatagattgt tccgctattt tataagtaga
     7261 tacctgtttg ggatcattaa gctgaagcaa tctactatga aaaggagtcg gttatggaaa
     7321 aggtaatgca atctgaccgt ggattcaaat ccgtgttggc tgaagtttac caaaaaaata
     7381 tggtaaaagg attaagtgcg ggtgttgttt tacatgcttt cattatcgga acgattgtgt
     7441 gggtaatcgg ccaaaaccca aaagcagtta agcaggtcat gaaccaaata tctatactgg
     7501 tggatatgcc acctttaaca aacattccgg aaattcctgc gatcaattta cctcaaaaat
     7561 ttaatccgag cgacattatc aaaggaatcc ctatcatcgc tcctgatgcc atagtttctc
     7621 ccgatattac gatacctgcg caagacgact ggattgaaaa gttctctgat aatagtgttc
     7681 cgatggattg gaagaatctt gatgtgagct taccgaattt tgaaaatgtg agtatcgata
     7741 gtaaaaacac aagttcaggc gaatttacga tagtggagca gatgcccgag gtgatacatc
     7801 gcgtgaagcc cgaataccca tctcttccaa gacaagccgg cattgaaggt agagtatatg
     7861 ttaaggggtt agtcaacgaa gaaggtatgg taacccgcac ggtttttgtt tcaggggatg
     7921 agatgtttta taacgcggct tcagatgcct ttttgaaaac acgtttcaaa ccgggaatca
     7981 atggcggcgt agccgtaaaa gtatggatta tgatgccctt tacttttcgt ttgcgttaga
     8041 tcaaagggtg tttctgtaag ttcagagaca cccttttgat gatttaggca acagatttta
     8101 gcgaattatt gcgtttggtt aatcggaaag cgtcatcatg gaacgcaagt aatagttgat
     8161 catttggaac aaaagctcct ttgagaagct ctgtggatat ctgagttacg acgtcctttt
     8221 ggatcaaccg ttttagcgga cgggcgccaa atatcggatc aaatccgagc cgggctaatg
     8281 catccaccgc cgtggtgtcc aattcgatcc ctatgttatg tttttgtaga agttttttta
     8341 catgagcaaa ttgtatatgt acaattcgct gcaaatcatt gcgttccaac ggcctgaata
     8401 cgacgatatc gtcaatccgg ttgataaact ccgggcgtaa acgttggcgg agcagggata
     8461 tgacggattc tttgatacgg gcgtgaatgc gatcacggtt ttcggacgcc atatcattca
     8521 ttttttccat gattacagaa gcgccaagat tggatgtcat gataatgatg gtattacgga
     8581 aattaacggt acggccttta ttatctgtaa gatgcccgtc atccaatact tgaagtaagg
     8641 tattaaaaac ttcgggatgt gctttttcta tttcatcaag cagaataaca ctatacggtt
     8701 ttttgcgaat ggcttccgtc aactgaccac cttcgtcgta gccgatatag cccggaggcg
     8761 caccgatgag gcgtgaaacg ctgtgttgtt ccatgtattc ggacatatcc agtcgtacca
     8821 tagcagtttc atcgtcaaat aaaaattcgg caacagcttt ggctaattca gtttttccaa
     8881 caccggtcgg gcccaagaaa atgaatgagc cgatgggtct tttttcatcc gataatcctg
     8941 cacgcgatcg tttgatcgca tccgaaacag atacaacggc ttcgttttga ccgacaacgc
     9001 gttcttgaag gcgctccggc atgcttaaaa ttttatcttt ctctgattct aacatgcgag
     9061 agacggggat gccggtccac ttggcaacaa tctcggcaat atcatcttca ctgacctctt
     9121 ctttgagcat tttttggttt ttctgaactt cggctaattt atttttttcc ttttccattt
     9181 gtgtaccgag ttcagtgagc cgtccgtatt tgagttccgc ggctttattg agatcaccgg
     9241 cgcgttcgga ttgttcgatc tgcgattttg taatttctat ttcagattgg acgcggcgaa
     9301 tttgttcgat aacttttttc tccgtttccc aatgactacg cagcgccgta cgtttttctt
     9361 ttaggtcagc gagttcgctt gaaattttat ccagtcggct tagggatacg gtgtcgtttt
     9421 cttttttaag cgccaattgt tcgatttcca gttgtttgat ggtacgctca accgtatcca
     9481 actcttcagg catcgaattg atttcaatac gtagcttgga agccgcttcg tcgatcagat
     9541 caatggcttt gtccggcata aaacgatcgg ctatgtaacg atcagaaagc acagccgccg
     9601 ctatgatagc gttatcttgt atgcgaacgc catggtgcac ttcgtatttt tctttgagac
     9661 cgcgtaatat agagatggta tcgtgaacag acggttcatc cactaaaacc ggttggaacc
     9721 gccgttcgag cgccggatct ttttcgatgt gcttgcggta ttcatccagt gtcgtagctc
     9781 cgattgcacg taattcgcca cgcgccaatg ccggtttaag catattagct gcatccacag
     9841 cgccttcggc cgcccctgca ccgacgagtg tatgcagttc gtcaataaaa agaataattt
     9901 caccttggga ttcggtgacc tcgcgaagta cggctttgag tcgttcttca aattcaccgc
     9961 gaaatttggc gccggcgatg agtgcgccca tatctaaagc catgacggtt tttgttctga
    10021 gattttcggg aacgtcgccg tcaataatgc gatgggcaag gccttcggcg atggcggttt
    10081 taccgacacc gggttcaccg atcagtacgg gattattttt ggtgcgtcgc gacaaaacct
    10141 gtaaaactcg acgaatttct tcgtcgcgtc cgatgaccgg gtccagtttg ctgcgtcggg
    10201 ctaattcatt cagattgcgc ccgtattttt gcaaagcctg atatttacct tcgggatttt
    10261 ggtcggttac gcgctgcgcg ccgcggacat ctcgcaatgc acgataaagt gtctcacgcc
    10321 atatgccgaa acctttgagc atttttccgg attccgtgct ttcctcagcc atagcaatga
    10381 gaagatgttc ggtgctgatg tattcgtctt tgagtttttc cgcttcttta ccggcttcga
    10441 tcaatatacg gctcgcacga cctgacaaat aaacctgttg tgcggtatcg gaaacacgcg
    10501 gcatgcgatc aagtatggat tcggcctgcg tgcgtattgt agcaagcggg gagcctaatt
    10561 ttttgatcaa ggcaggaacc gtaccgtcat gatcgtccaa catcgccatt aaaatatgct
    10621 caggttcgat ctgcgtatga tgccgctcca atgctaactg ctgggcctta gataaagcct
    10681 cttgcgcttt gattgtcatt ttatcggaat tcatgagtta cgttcctttt cattgcctta
    10741 ctgcgagtgc ggcgtattcg tgcgatttat ttgttttttg ccaatgatca agctgtcatg
    10801 atcatataca atgccagaca aatcataaga atgacactta ttttaagtcc gtaggccatc
    10861 gcattttgct ctgtaaataa atgcgggatg gttggtacac gcatcccgcc gcaagttata
    10921 cttctgacga cgtgcatgcg acacgccgaa ttattttaga atttaatttc aatttctttc
    10981 gttttggctt cttcggcctt gggcaatacg atggtcagga tgccgtcttt ataattggcg
    11041 tcaatttttt ctcccttgac catggacggg agtcggaaag agcgcatgaa agagccgtag
    11101 ctgcgttcga cacgatggta attattttct ttattttccg attcctgctt tttttcacct
    11161 tggacagtca gcacattatc gtgaatcgtt attttcacat catttttgct gacaccaggt
    11221 aattcgatac gaacagtata ttcgtctttg ccttcaacga tatccgttgc cgggttccaa
    11281 ttggttaggc cggtatattc gtctcgatta aagacggaat taaaagcctg gttcatttct
    11341 ctttggatcg caaacaaatc agagaagatg tcgcgatcgc gcggggtgaa tttgatgagt
    11401 gacatggtca atctcctttg gttgtttggt gtttagttaa agttaattgc gatttcacgc
    11461 ggcttcagtt cttctttttt accaacgctc aggatcagaa atccatcttg ccaacgggct
    11521 tcaatcttat tgggatcaac gcggtttgtc agtcggaata cgcggtgata gttgcgtttg
    11581 ggtatttcct cgcggataac ggatccttct tcatttaagc ccattttgcc ttcgatcaca
    11641 agattgccat tttgcaaagt gacggtgagt ccatccttac gtactccggg catttccaca
    11701 tataacagat agttgtcttt gttttcgcga atatccgtac gcggttgaat gtagtagtaa
    11761 ttattttccg gcttggctat tgcttgtgtt ttcacttcat attcctgttt ggtttccata
    11821 agtgctcctg cgttttagta tgttggatat tttttgtgct cggttaaagg aattacaaac
    11881 ccaatgccaa aaatatcaaa attaaaaaaa acaaaatttt attaacagcg cttatagccg
    11941 acacaataat tttaacgaca aattgtcatt gtttttagtt tccgatacga accaaatgtc
    12001 attttttatg acaaaatgac tgctgtcgtt aattgattta gaatgagtga attttttaca
    12061 tcagaaaaag gagagcatga ttaaaaatga atgccttaat cgaaaatgaa gcagagtaaa
    12121 ttttatggag tcgatttttg aggtatttac aggagcgtga tacaatcact gaagttcatc
    12181 ttgtctttgg tttataaaag aaatattatt aggccaatat tgaaaaatat gaacgaagaa
    12241 tttcaaatac gtttagatat cgtcgaatgc gggcgcagga tgtatgcacg taattatgtt
    12301 gcatccaatg acggcaatat cagtgtgcga gtcggtgata ggatcatcgc aacccccaca
    12361 ggtataagca aggggtttat gcgtgcggag gatatggttg ttttggatat ccaaggccgc
    12421 gttatcgaag gggcgcgcaa accctcgacc gaaatcaaaa tgcatctagc gatttacgaa
    12481 gagcggaagg atgtaaattc cgtggtgcat gcgcatcctg ttcatgcgac aggttttgcg
    12541 acggcgcaga tcgaactcaa agattgtgta ttggcagaaa tcgtaacgac gctgggttcg
    12601 atacctttag cgccgtatgc cacgccttcg acgacggaac ttcccgattc tatacgtccg
    12661 attattcgcc gcgctgatgc atgtttatta gccaatcatg gcgttgtaac gtgcggtagg
    12721 gatgtgtatg atgcgtatta caaattagaa cgtgtcgagc actatgcgcg tattttgttt
    12781 gtggcgaaaa gcctcggcgg tgcaaaaata ttatcgccgc agcaagtgca taaattagaa
    12841 accattcgtg ccacgtatgg tacatccgat gcgcataacc ccggatgcat agcgtgccat
    12901 ggcgcttgca tcggaagcga ttgtacatta tacgaaaccc aacagcgtga tcaatccgca
    12961 tcggatttgc atgtggaaga aaccgttcgc gctatcattt cagccaaaaa ataaatgaat
    13021 actgaatttg gtaatcgctc acctttacga tcgctgttga cgataatagc ggcggttgtt
    13081 tttacatttc tcatgattat tgcagcgacg atcgcggcgc tgttcggacc gcgttctaaa
    13141 gcaacgcatg ccgtcgaaaa attttgggcg cgaacgatac ttcgtatggc cggtgtacgc
    13201 gcgcgggttc gcggtctgga gcacattcgt ccggatcatg tgtatatttt tataagcaac
    13261 caccaaggct tatttgacat tccgctcatg atggcttata cccccaaaca gctccgtatg
    13321 atccataaaa aagaattgtt atgggttcct ttttttggat ttatactttg gttgcttgat
    13381 tttattgcca tagaccgagg gaaccgtcag cgtgcagcag aaagtcttca tcgcgcggca
    13441 aaaaaaatac atgacgggac gaatatcgtt atctacgccg acggtacacg cagtatagat
    13501 ggagagttac gtccattcaa aaaaggggca ttcatattgg caatcgacgc acaagtggat
    13561 gtggtgcctg ttacgatcag cggtacgatc aatgttatac ataagtttga aggagtgttc
    13621 gatattcgtt ttgggcaaac ggtcgaattg gttttttcgc caccgataag caccgatgga
    13681 atgatgccgg aagccaaaga cgatctgaaa gatcaagtac agaacattat cgccgccgaa
    13741 tattccaaaa tcaaacatct ttctgcgata accaatcctg ctatactatc ccgcttaaaa
    13801 aaataattta gctttgtgaa agttgtcaca taaacacgcg caaaggcagg atacgcaaag
    13861 tgtgtttttc ttttgtttga aatgtatttt tgttattatg gcggcgcaca aaactaagtg
    13921 agagtatgaa acgcatcggg ttatttggtt ctacgggctc tatcggcgag aatgttttgc
    13981 aggttatacg caatcatccc gatcgtttta ccgtccaatg tctaaccgca aacaccaacg
    14041 ccgagttgtt gatcaaacaa gcgctggaat ttaaaccgca atctgtagct atcgtctccg
    14101 atgaagggta cgcccaattg catagcggcc tatccggtac aggtatccgt atttatcatg
    14161 gacacgatgc catcgtacaa atggcgcgtg atgaaaatta cgacctcatg atcggcgcga
    14221 ttgtaggatt tgccggattt ttacctacga ttgaggcgat acgtgcgggt aaagatatag
    14281 gtttagccaa taaagaaacg ctcgtcgtcg ggggcgctat cgtcaacgga cttctgagca
    14341 aacaccaatc gcggcttata ccgattgaca gcgaacacag cgcacttttt cagtgccttg
    14401 tcggtgaaga tatgcgcagc gttagaaaac ttattctcac ggcatccggc ggcccctttc
    14461 tgcatacccc ggcggaggat ttttcatcga tcaccgtagc ccgcgcttta aaacatccca
    14521 attggtccat gggcgctaaa ataacgatag attccgccac gctgatgaat aaagggcttg
    14581 aagtcatcga ggcgcattgg ctttttggtg tcggggcgga tcgtattgac gtggcgattc
    14641 atccgcaatc tataattcac tctatggttg aatttgtgga cggctcgacg aaagcgcaac
    14701 tcggtgtacc ggatatgaaa gttcccatac aatatgcgat gacctatccc gaacgcatga
    14761 ccaataattt tgacgtattt gattttcgta aatggaatag acttgatttc tatgaacccg
    14821 acaggaataa atttcgttgt ttgaagttgg cgtatgatgc gcttgaaaca ggcggttcta
    14881 tgccggcggt gctcaacgcg gccaacgaaa tagccgtcgc gggattttta caagagaaaa
    14941 ttcgttttac ggatatagca tcgtcgatcg aatcggccat gcaggctcat acctgcgtgt
    15001 gcgatccgga tatcgcgtcg gtgattgaaa ccgatcggtg ggcgcgtgcg tatgtacaaa
    15061 aaacttgttt ataacaactt gagtaaaata taaaggaata ccatgagcat tatttttgac
    15121 gcgttaattc cgtttctgat tgtcctcggg attctggttt ttatccacga actcgggcac
    15181 catctggcgg ccaaattatt tggtatgaaa gtggacgcct tttcgttggg ttttcctcca
    15241 agagcgtggg ggtttcactg ggccaccaaa aaactacgcc ctaagttcat caaagatatc
    15301 acacaatcta ttttgtccaa taccacattc caatcattgg ataaagaaat acaagccgcg
    15361 ggtttggcgc agtcatccga cgcatcggtc aaatttctag atacgatctt tgaaatcaca
    15421 caggtcgaag aaaaaatcga actaatcgac gctaaaggga aaaaagaaga aaaaataaat
    15481 ctccgcacgg aattgcggtt tgccgaaagc ggtcctgccg catcattgac caatctacaa
    15541 cgcgatgcat ggctggtgcg tgtccagggt gatgccgatt tattggatga tcttctgaaa
    15601 tatggcgaaa taaacgacga taaaacgttt cacgaaaaat ataaaacgga ctactgtctg
    15661 agttgggtgc cgctcggcgg atattgcaag atcaatggta tggtggatga gagtcttgat
    15721 gaggacagca tgaaagaggg tactcctaag ccatgggaat atcgtgcaaa gccgatctgg
    15781 caacgtatga ttgtcataac cggcggcgtg tttttcaatt ttctcctggc ggcttttgtc
    15841 tttacgctga tgggactcgt gcgcggtttg ccggatatgg aaaaatttga taactggcgg
    15901 cgcgaaaatg cgttgcccgc ggaagtcggc gtcgtaagcc ccggcagccc tgcagataaa
    15961 gcaggcattc aggccggcga tcgtatcgcc agtattaacg ggcaaaccgt acaaaaatgg
    16021 gatgaactgg tggatgtgat tcgccataat ggcggaaaaa aacttccgat cgaatggacg
    16081 cgcggttcgg aacgatttag cggcgaagtc atgccgacga ttgataaaat tcaaactgag
    16141 aaaggtattg aagaaatcgg acggatcgga atacaggcgc ccccatttcc tgagtttact
    16201 cgcgatgcat cgttcattga agccgtacag catgggttca taacggccgt gggcgcgacg
    16261 tattttatcg tgcgttcgat caaacaaatg gtttccggcg aggtcaacgc caaagatgcg
    16321 cttggcggtc cgcttatgat cgctaaaacg accggcgaca tgaaacgcca atacggttgg
    16381 gaaggcattt ggaatctgat ggctttgctt agtctcagtc ttgctgtatt taatctgtta
    16441 cccgttcccg cgctcgacgg cggccatctt tttatgttgg ctatcgaagg tgtcatgcgc
    16501 agaccgttgt cgctcaaagt taagctccgc gcgcaacaag ccggcatggc tattctgcta
    16561 tcgctgatcg tatatatttt ctttaatgat atcgtcaaac ttacaggtat tttttgaaat
    16621 ttacactgct tcataaagat cagagcggcc gccattcgcg tgcacgtgcc ggtattctgg
    16681 aaacggatca cggtaaaata gagacgccca tttttatgcc tgtcggtacg gtgggcgatg
    16741 tcaaggccat cgaacatcgt gaactgaaag aaaacggggc acagatcatt ctcggtaata
    16801 cataccatct gtatttgcga ccgggtacgg agatcatcca gaaagcaggc gggctgcata
    16861 aatttatcgg ttgggatttg ccgatattga ccgatagcgg ggggtaccaa gtattcagtc
    16921 tttctcagct tcgtaaactt tcacgcgatg gtgtcaaatt tcagtcgcat attgacggat
    16981 caaaacattt ttttcgcccg gaagatgttg tggatattca gcggaaactc ggttcggata
    17041 tttgtatggt gctcgacgaa tgcccgccgt atccgtgttc gcatgaatat gcggcccgtt
    17101 ccaatcgcct gaccatcgaa tgggctgaac gctgcaaaac acattttgaa aaatcaccac
    17161 cgttgtacgg tcatagccaa acactttttc ctatcgttca aggcagcgtg tttgaggata
    17221 ttcgtctcaa aagcgcagag gctttggtta aaatggattt tgaagggtat gccataggcg
    17281 gcgtgagtgt cggtgaacct aaggaagacg tttacaaaat agccgagatg aattgcgccg
    17341 tattgccgga gcataagccg cgctatctga tgggcgtagg gacgccgcaa gatatcttag
    17401 aatgtattga gcgcggtgtg gatatgtttg actgcgttat gcctacacgc aatgcgcgca
    17461 acggaacttt gtttacgaca tggggtaaga tgacgattcg taatgccgct tacgcggatg
    17521 attttacgcc gattgatgaa aattgcggtt gttacacgtg tcgtaatttt accaaagcct
    17581 atgtacggca tctgtcaagc atcggtgaga ttttgggtca tcaactgacg acgattcata
    17641 atatttattt ttatctatgg ctagtcaaag aagcacgtaa acacattttg caagatacct
    17701 atacggcttt caaaaccgat ctattgaagc gatacgaaga aaacgaaaaa aataggggaa
    17761 gcgaattggc gcgtcatgcc aagaccaaac gtaagcgtaa agattatgaa actagtggcg
    17821 cctgatgctc tgaaagcatg gattgatgag ggcaaggtaa tgatgttatt ggatgtgcgc
    17881 gaaccgtggg agcatgcaat agccaaactt cctgattcgg tgctgattcc attggggctt
    17941 ttaaacgtac aatcggaaag actgacaggt ctggataaga caacacccgt agttgcgtat
    18001 tgtcaccacg gtatgcgaag tcaaagggca tgtgctattc ttgaatcgct gggttttacc
    18061 gatgtataca acctgaccgg tggtattgat tattattcgg attgcgttga tccgaccatt
    18121 gaaaaatact agttattcta tcttcttttt cggcatcttc ggtaaagact tattaagagt
    18181 ctcttaattg taacaaaaat cccttcaatc aaattaataa tgactgatgc agtcttttat
    18241 ctttcagtgg gttcccgctt tgcctcgact ggcgttcgaa cacgtctcgc tttgatacgt
    18301 cacaccatcg gcgtgactac tcagcgcatg ttggcgtaac tcacgtcgag gtctcgggac
    18361 atgtcgcggg aacgacaaga cgatggtttt cgtattgtct caccctcaca cgcgggtgtc
    18421 cactaaaata ttacaaattc aattttttat aagataaaac ctgacttagg tcattgtttt
    18481 tgaatttttt tttagtacgc tttctacgta gcgtatttct attgagggac tttctatgaa
    18541 tctctcttct tcgcaaatag cttttccaat gtatctctct gaatgaaggc gatgacatat
    18601 atgaatgaat gtttccatgc tttatt
//
LOCUS       DAOQGF010000063        18328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1413_length_18328_cov_7.009030, whole genome shotgun sequence.
ACCESSION   DAOQGF010000063 DAOQGF010000000
VERSION     DAOQGF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_18328_cov_7.009030"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3957
                     /locus_tag="PK937_09715"
     CDS             <1..3957
                     /locus_tag="PK937_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH32888.1"
                     /translation="YVATASYGTTSSAGLSISAVTLGNNIANATSAYTIDFVLSATGA
                     LSTSDSVVMIFDSSTVFPSSIANNAITISAGGFSDYVTGSTVSKQTASGQTRLAMRMP
                     VSAANSASVSVQIALSTNIQNPAVSGNYQLSVKTVNSGGATIDNAQASNSYNIAAATT
                     TVSSSSVALSNTTAAQTNVSYTLGFTLGSYGRMLSGSSVFKVKFPSGTGYGTLTGTVN
                     GTSASTPTRSGDTVSVILPSTVSLSNSASVTLVIGGITNPANGSYTISLATSVEASYV
                     VSQSFTIGGTAVTISSVTLGNSGVNQTSAYTINCTGVSNLNTGDIVRVTFPEGTTVPS
                     SIAAANVTFTGGTGQTVTSVATNTSSRFADITVGSNNKTFAAIVFASGAGIVNPSVPS
                     TTFYKVNVVTTTNTQPATSPAYTVSSNTTSVTADTVVVTSNVVSQTNAIYEVYFTTGA
                     FGKLAGGTSAGSDSIRIRFTSGGVVVPSSITAANVTINGVSSTGVRVVTSGANGEVKI
                     EMPSGASVPASTQSKVTFSASAGLTNGGSAGSYQVQVATDAENTLSTKTSNMVFAASS
                     ALAVTQVTPSPSTINATAAYTIKFTTGSSGALAINDSILITFPSNTYLPGTISSSLIT
                     VNGSQLAASAVGSNNVLRIRNPVTINNVANVTIAISSSAGILNPTGVSTSYSLTVTTK
                     TSGGTVVEGPTTSPQYTTTATSTTITTPSVTLSSSTPSASSNYTIAFNTGTNGRLRST
                     TDSVSIVFPSGSTVPASPTVTINTVSASAYGTGLKVTAAIPASVSIGNSGSASVVING
                     ITNPTAGNYTLSAYTTVESGSITSPSYTINNAPLLTGVSLAFTPNTDTVNMAGGDSIY
                     FTTGTGGALTSGSSTITVVFPNNTVIPASITASNVTVNSVAASVVATNSGTRTVTVTS
                     PTNVNASTNVTLFFATACGIINPSVAGTYAVQVSTTPQPNLTTSSNYTVKAATTTVTN
                     FTMTLTQSGTSDGPNEFGRYNYTFKTGLRGKLLSGTSTISLLLPYDATFTTGTPTVST
                     VTVNGTAAAALRLGLSTLNEDTLEVTVPASVTIGNQTSVTVIIDSTSGLRNASTASAL
                     TYKVFTSVETGVVGGDATLPVELAFFDVVQNGNKAVLKWRTESEINNAYWLVERAESN
                     FIENEDDLQGKTSHLQYTRVAEITGQGTKNTSTDYILNDDKVEIGKTYVYRLMDVSYD
                     GVLSIHQEKKIIIETPQVFDVFPNYPNPFNPSTLIRFQIPSESHVVVTIYNLLGQEVK
                     ELVNKDLPAGMHTMQWNGTNNRNSLVASGVYLYRVTATSLQNKSKYSRTHRMTLLK"
     gene            complement(4000..4536)
                     /gene="ruvC"
                     /locus_tag="PK937_09720"
     CDS             complement(4000..4536)
                     /gene="ruvC"
                     /locus_tag="PK937_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNH32889.1"
                     /translation="MVILGIDPGTNTTGFGIITWERNKVNWVASGTIELPEKETLARK
                     LELIYDHVVDLAHTYKPNECAIENVFYSKNVKSALKLGHARAAAMLGALHKAVHISEY
                     SPKDMKMAVVGNGNASKEQVQYMVKMVLGITKEMSFDESDALGLAICRAQRIKTPRAS
                     VSSWKAYIKAHPDRVKVK"
     gene            complement(4536..4940)
                     /locus_tag="PK937_09725"
     CDS             complement(4536..4940)
                     /locus_tag="PK937_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32890.1"
                     /translation="MNRSLKLLFFAALVMNLSCREPLVNSEDAKLENNLVGTWILVST
                     FPETPGEIQLTFRDDLSLHVYSKTATNPPVVRDEEWHFFVDDKQIVMFRDEIDTNGQP
                     YTQYDYYPIKSLTHKELVFVNLERIQQTYARN"
     gene            complement(4930..5802)
                     /locus_tag="PK937_09730"
     CDS             complement(4930..5802)
                     /locus_tag="PK937_09730"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNH32891.1"
                     /translation="MNLSQKNNWRVGILGAGRVGSSIALALSGNKIPILSVVSATSHS
                     AAVLAKKINCKIFSNKIEDLRTCNVIIVSVPDRQLKAITKRVMQIRFIHNSVLLIHTS
                     GMYGATIWNQRTSENKEVKFWTAAMHPLQTFVTRMDTSPEIFKTYFALDGKNKPVSIV
                     KHIVKIMGGKSIMISDKDRATYHASAVIASNFLVALSHASTELLRQIYPQSSGLEKLF
                     LPIQQQTLANISKHGAAASLTGPAMRGDKQTIRLHKKAIQKHSKDILKIYTTLTSYCI
                     ALSEKRQRGKINEP"
     gene            5895..6125
                     /locus_tag="PK937_09735"
     CDS             5895..6125
                     /locus_tag="PK937_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32892.1"
                     /translation="MFDTYSSKVELAQSDFNEIAEQYFQNYLSSWCDHNKIDDASRKA
                     MVTVLSDYSNFFFDALMQSTIEQHADRTELKN"
     gene            6127..6525
                     /locus_tag="PK937_09740"
     CDS             6127..6525
                     /locus_tag="PK937_09740"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNH32893.1"
                     /translation="MSGSVIHNKHYSPEEARILLHDIRVKINTLVALKKHLDEINYNI
                     YKHEYFGGLGPNGSKFHPEELMTLVDILRSLEKQGVIIKSIDEGLIDFPAIRANGEEV
                     YLCWKLGEEDIFYWHTIRDGFQGRRPLSAF"
     gene            6595..7257
                     /locus_tag="PK937_09745"
     CDS             6595..7257
                     /locus_tag="PK937_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH32894.1"
                     /translation="MARAGSIIRSSSGKDIFTRRDKDFFEFFGQLESVENKFNAYLTR
                     FKYPKREKYLIRKAYDFSSLAHKGQLRNDGAPYIIHPIRVASILLHELSVTSADMICS
                     ALLHDVIEDCGITENEIKNNFNESVASMVRTLTKNPNLSNAKRVYFEAIMQAEDHIRL
                     VKLCDRLDNLRSLRLIRNKAKTTRYIKETEGQYLPIAAEISPYLFRELRFEIQYQRTR
                     YR"
     gene            7297..7821
                     /gene="tpx"
                     /locus_tag="PK937_09750"
     CDS             7297..7821
                     /gene="tpx"
                     /locus_tag="PK937_09750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNH32895.1"
                     /translation="MKERAGHLSIKGKPLTWLGEEIKAGQKAPDYRVRTGFGPEATVT
                     LADSKGKIRIISVAPSLDTGVCEMQAIRFNQEVEKLGADIEVHQITVDLPPAMTRFCN
                     TKLNGSVKIKTFSDYAERSFGQAYGFLIKEWQTLGRGIVIIDKNDTVAYVEYCPKIEE
                     LPNFDVALAALKGL"
     gene            complement(7904..8329)
                     /locus_tag="PK937_09755"
     CDS             complement(7904..8329)
                     /locus_tag="PK937_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32896.1"
                     /translation="MNAKSKPFEETGLAAHLLSQFTSMRRTTKKSVQTAPALANDFFS
                     KDILTMLDFLQPLGTDIIATTASPPSHTIESFFNSSLSTRRAFRSAPVKTEPEWTPDE
                     VISIDMVETKANPTTSPLAQEEAPARPRTIEEFINGLFQ"
     gene            complement(8335..8994)
                     /locus_tag="PK937_09760"
     CDS             complement(8335..8994)
                     /locus_tag="PK937_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32897.1"
                     /translation="METTSYEFTLPENYTAFSPVSAEIFVQNTNRSFAVDTVFLKALQ
                     QSFARLYAAHDQDALYRTGVQWGNSVYAKIETLALTMHPDAKSIREIPMPDFHRLFTN
                     HLAALGWGNFELKRRDDFLFVDLYHSLWVEMLQSQSQAHGSKPHTSCALYAGFFAGIF
                     SRISNMPLACIEITCKNEGYEHCSFLLDNEETIAMVKARISGKTTPLDAFEKVKKEFE
                     G"
     gene            complement(9017..9850)
                     /locus_tag="PK937_09765"
     CDS             complement(9017..9850)
                     /locus_tag="PK937_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH32898.1"
                     /translation="MGQIFSIASQKGGVGKTTTALSLGIAFSRLNKKTLIIDADPQGG
                     ISFCLDRYYDMRRDQDQGRNGLYQVFCGAMDIAEAAQPSMIENLSVIDCGISARSSDI
                     ELYEDATRATGLFREAVIQAAQHYDTVLIDCPPGIGLVSAGALAASDYVIIPVQSEPL
                     SLRTLPQLLRQLIAIKKEHNPDLAMAGILLTMYDHQSPASEMVAQQIRDFFEEDIVFK
                     QAIPRDPVINLLFAGHENITSVLTEIEAQSVAFQAYQALASEIAIQFSETPLRLNSIS
                     H"
     gene            complement(9858..10502)
                     /locus_tag="PK937_09770"
     CDS             complement(9858..10502)
                     /locus_tag="PK937_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32899.1"
                     /translation="MPTNPIEAAIRNHMQIDHATGSIALSDFFTPTARRIGIADYDFF
                     EALFSVFSENSTPDQTRERFYQFGADWGNSFLERLKTQLAQAGGEHASEPQALLKDEF
                     VEHLNSNFSYLGVGQFRILEGNKFYIIDLKNPWRPSDEHKQIYASTSFAGFFSTLFSG
                     IAGRALSSTFLSNTPEQWRFALSTTEVIEDIRTQINAGLTETDILTQYHNQHLL"
     gene            10635..11984
                     /locus_tag="PK937_09775"
     CDS             10635..11984
                     /locus_tag="PK937_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNH32900.1"
                     /translation="MQELSNENNVHLVKRVASVVVTIIIMLALRYSTLITESDPSVFS
                     VTLLGLMLVLSFNIGKLLSRLNLPTLSIYLITGILCGPFVLGFLSSSTIDNLKFIDTL
                     ALGLIAFIAGGELRLKELLRMKKVLVSISLMETLMVFIFCAVGFIVLTPLMPFTQSQT
                     FLLNSLIALIMAALLVANSPAVTIGIIGEYKSKGLLTETILGSVIFKDIIVIVLFATV
                     TTFVMISLDSTAEWSMMVFIKTIGYEIGMSIVAGLGVGFLVYLYIRFIGEQPVLFVIG
                     AAFMSYEIARQFHLEVLLVGVSAGFAVQNFTKQGEALVEHVEESLPIVYPIFFAIAGA
                     KINLPLMLEMWYLALFLAVIRTLGIYFGVRKGARMASAAESVQKYAWMGFVSQAGVAL
                     GLATIVNYKFPEWGGYLQTILLSVIGINQLVGPILLQKALDKAGEINKPMPQGSILN"
     gene            12012..12536
                     /locus_tag="PK937_09780"
     CDS             12012..12536
                     /locus_tag="PK937_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNH32901.1"
                     /translation="MAKFVVHIAKEYLKFSSAHFTIFSDHALEMLHGHNYYVTLDAEF
                     RDSANGITIDFKHQKKIMEALCDELDEKVLLPSESPYLKIEKITDNFRVVFHGEGFTK
                     EYQFPCEDVQILPVNNISSEMLAKQLCESFCEKLMQTWQHLKPGSDISEVLLAVHVGV
                     EETRGQSVKYVWEY"
     gene            12539..14074
                     /locus_tag="PK937_09785"
     CDS             12539..14074
                     /locus_tag="PK937_09785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH32902.1"
                     /translation="MAELELKEFEKTICARQLTIDDYESVTELQIRSFPGMKPWSYDQ
                     FESQIRLFAEGQIGIEYNGLLVASSSSLILDFDPYKDWHSWKEISDNGYIRNHIVNGN
                     TLYGIEIMVDPGYRGLRLARRLYEARKSLARKYNLRSIILGGRIPGYDKFADQMDVKS
                     YVEKVSSKVLVDPVLTTQIANGFVLKRIIPNYLMSDTESRGYATFLEWTNLDYVPDFA
                     GEKQKTIVRISAVQYQMRHIRDFEDFAKQCEYFVDVASDYRSDFILFPEMFTMQLLTF
                     LPNLRPGLAVRKLAEYTPKYLELFNHLSVKYNINIIGGSHFTVENEDLYNISYLFRRD
                     GTIGKQYKLHITPSERKWWGVKAGHKLEVFDTDCGKISIQICYDIEFPELTRVAVEKG
                     AQIVFVPFCTDERYAYLRVRYCAQARCVENQIYTVIAGSVGNLPYVENLDIHYAQSAI
                     FTPSDIPFTRDAIQAEATPNIETVIIDEVNLETLRHNRQSGSVLNWQDRRTDLFKVNY
                     QGS"
     gene            14115..15182
                     /locus_tag="PK937_09790"
     CDS             14115..15182
                     /locus_tag="PK937_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNH32903.1"
                     /translation="MKSKTIKKNKPIVKKNSVKTQPKISESKNQTPYRALGLAPDDLL
                     RMYRLMKLTRQFDQGIIRLYRQNKMIGGAYSGWGNEATAVGSAYPLLPHDYLYPMHRD
                     IGAHFTKGQSAKILMLNHLARAEGPTRGVDGTGHYYDKNLRIVGNISHLAAMIPQAVG
                     TALASVKKKENAVVINYVGDGGMNVGEFHEGLNMAAVWKLPFILIIENNQYAYSTPNT
                     LQFACESPVDRALGYGIPGVKVDGTDILEVYRVCKEAFERARRGEGPTLIESVTMRMR
                     GHAEHDGHEYYPPGILEKWEKKDPIMQFEKFLKSEKLITDKMIEDIDTSTKQEIDEAI
                     EYADKAPWPEPSQAATWVYAE"
     gene            15222..16250
                     /locus_tag="PK937_09795"
     CDS             15222..16250
                     /locus_tag="PK937_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNH32904.1"
                     /translation="MTGQSKTSTKPYAHSTELDLKKYEPTKEQMKEMYYFIMLNRAFD
                     DRIAKLYRQGKIIGAAYGSRGQEATSVGSTYALGPDDIVGPIIRNAGSIIVRGLPVKN
                     FLSNFVGRSTTPTRGRDGNSHLGDLNMGVFAPISHLGSLISNIAGCALAFKMKNEPRV
                     ALTYIGDGGTSTAEFHEGMNMAAVMKLPFICIIENNNWAYSTPTQTQAMIKDFAIKGK
                     AYGMEAIVVDGNDVFEVFRTTRYAAERCRKGKGPILIEAKTMRMKGHAEHDDAFYVPK
                     EQFAEWQKKDPIARAEKFLLDKKYMTATENEALKQRVAKDMEEAEEFALNAPFPAMDE
                     AATGVYAD"
     gene            16263..17324
                     /locus_tag="PK937_09800"
     CDS             16263..17324
                     /locus_tag="PK937_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH32905.1"
                     /translation="MKKLFLIFVLFVHSAFAQTSFAIIPFTGTADVGVNDREAFVSGL
                     YQGMTNSGRYRIVDRNLIEQVLKEQNFQLSDLTEQKKVIEFGKIAGAEKIITGKMFRY
                     SSTQPAITFSVIDVSTSQVEFSKEITYNNYTFSNLSSFLVSQLIDKYPLVGRVLGKSG
                     DVYIINIGLNQNIKNGTRIFVARNNVLKGDDGEILMEEYKRVGILEVTLADKARSQCK
                     LRSLVESNVIIQKDDMISPDPIPERPALVLDKPFWSGYSKGELLLDDDMSKKQYLSVT
                     SALGETYKDGRLFINTTHLNSGHGYCYYPAPFDAVGDFVCEAEITFEKNTEKYNRVTF
                     IFRNVGAYGEGNGYSLPVV"
     gene            complement(17326..18186)
                     /locus_tag="PK937_09805"
     CDS             complement(17326..18186)
                     /locus_tag="PK937_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HNH32906.1"
                     /translation="MVHEHLSDMLTAQGNIPKPGPRPKLSDAAIIALSLTSEILSIDS
                     ENRLFMQLRQYPKVFPTLIDRSGYNVRRRKLFSYTNHLRNTILQKISSSESVFIIDTM
                     PLPVCKLARANRSRICKESFETAPDKGFCAAQQTYFHGYKLHSVCTQKGVFTSFDLSK
                     ASVHDIHYLKNVKEHMANAILLGDKGYLSEDIQIDLFTSVRINLQTPLRSNQKNYVKQ
                     PSILRKTRKRIETLFSQLCDQFMIHRNYAKSFDGFKARILAKITALTISQFFNYQNQK
                     NLNLIKYAFI"
BASE COUNT         5207 a         3701 c         4255 g         5165 t
ORIGIN      
        1 tacgtcgcga cggcatcgta tggtacaaca tcttcggcgg ggttgtccat ctcagcggtg
       61 acattgggaa ataatatcgc taacgcaact tccgcctata ccatagattt tgtactgagc
      121 gcgacgggtg cgctttcgac ttccgacagc gtagtaatga ttttcgattc gtctacagtt
      181 tttccaagct ccattgccaa taatgcgata acgatttcag ccggcggatt tagcgattat
      241 gttaccggta gtactgtcag caaacaaaca gcatccggac aaacacgctt ggctatgcgt
      301 atgccggttt ctgcagctaa ttcagcctct gttagcgttc agatcgcttt atcaacgaat
      361 attcaaaatc cggccgtatc gggcaattat caattatccg ttaaaacggt caatagtggc
      421 ggcgctacga ttgataatgc ccaggcgtct aattcgtata acattgccgc agcgacgacg
      481 acggtatctt catcatcggt tgcattgagt aacacgacgg cggctcaaac gaatgtgtca
      541 tacacgcttg gattcactct tggtagttat ggacgcatgt tatcgggctc aagtgtattt
      601 aaagtaaaat ttccatcagg aaccggatac ggcacgctaa cggggacagt gaatggtaca
      661 tcggcatcta ctcccacgcg aagcggtgat acggtgagtg ttattttgcc ttcgactgtt
      721 tctcttagca atagcgcttc cgttacattg gttatcggcg gcataaccaa tccggctaat
      781 ggttcttata cgatttcgct tgctacgtcc gtagaagcat catacgtcgt ttcacaatct
      841 tttacgatcg gcggtacggc cgtcacgata tcatccgtga cattaggaaa cagcggtgtg
      901 aatcaaacat cggcttatac gattaactgt accggtgtaa gtaatcttaa caccggtgac
      961 attgttcgcg tgacttttcc ggaaggcaca accgttcctt cgtccatagc tgccgctaac
     1021 gttacattta ccggcggtac aggtcagacc gttacgtcgg ttgcaaccaa tacttcatca
     1081 cgatttgcgg atattacagt aggatctaat aataaaacgt tcgcggctat agtatttgca
     1141 tccggcgccg gtatagtcaa tccttccgtg ccgtccacta ccttttataa agtcaatgtc
     1201 gtaacgacca ctaatacgca accggccacc tcgccggcat ataccgtttc gtccaatact
     1261 accagtgtca cggccgatac tgtggtcgta acgtcgaatg tggtcagtca gaccaatgcg
     1321 atctatgaag tttattttac gacgggagct ttcggaaaat tagcgggtgg aacaagcgcc
     1381 ggatccgact cgattcgaat tcgatttact tccggaggcg ttgtagttcc gtcgtctatt
     1441 acagccgcta atgttaccat taacggcgtc agttcaaccg gtgtgcgcgt tgtcacttca
     1501 ggagccaatg gggaagtaaa aatcgagatg cctagcggcg catcggttcc tgcaagcact
     1561 caatcgaagg ttacatttag cgcttccgca ggactcacca atggtggttc ggcagggtca
     1621 tatcaggtac aggttgcaac cgatgcagaa aatacgcttt cgactaaaac atccaatatg
     1681 gtttttgctg ctagttcggc attggccgtt acacaggtga caccttcacc ttccacgatc
     1741 aacgccacgg cggcttatac gatcaagttt acgacgggct catccggcgc attagccatc
     1801 aacgactcga ttctgattac atttccgtcc aatacatatc tgccgggtac tatttcatct
     1861 tcacttatta cagttaatgg cagtcaatta gctgcctcag cggtaggttc caataatgtg
     1921 ttgagaatcc gcaatcctgt aacgatcaat aatgtagcta atgtcactat tgctataagt
     1981 tcaagtgccg gcattctcaa tccgaccggg gtttcgacat catattcgtt gacggttaca
     2041 acaaaaacct ccggtggtac ggtggttgaa ggtcccacaa cttcaccaca atataccaca
     2101 acggccactt ccactacgat cacgacacca tcggttacgt tgagttcaag tacaccatcg
     2161 gcatcatcaa attatacaat cgcattcaat acaggtacca acggccgcct tcgttctacg
     2221 acggactctg tttcgatagt ttttccctcg ggaagtaccg tccctgcatc tccgacagta
     2281 actatcaata cggtaagcgc ttcggcttat ggcacgggtt taaaagtgac agcagctata
     2341 ccggcttccg tttcgatcgg aaactccggt tcggctagcg ttgtgatcaa cgggattaca
     2401 aatccaacgg cgggaaacta tacgttgtca gcttatacta ccgtagaaag cggctctatc
     2461 accagcccgt catacacgat caataacgct ccgctattaa ccggtgtttc gttagccttt
     2521 acacccaata cggatacggt caatatggcc ggaggggact cgatttattt tactactggg
     2581 acgggtggtg cgcttacgag tgggtcgagt acaataactg ttgtatttcc aaacaatacg
     2641 gtcataccgg cttcgatcac ggcttctaat gtgactgtca acagcgttgc cgcttcggtc
     2701 gttgcgacta atagtggtac gcgtacggta acagtgacat caccgaccaa tgttaatgcg
     2761 agcaccaatg ttactttgtt ttttgcaacg gcttgcggta ttatcaatcc ctcagtagca
     2821 ggtacgtatg ccgtgcaagt atccacgacg ccgcaaccca atttgacgac aagttccaat
     2881 tatacggtta aagcggcaac aacgaccgta accaatttta cgatgacgtt gacgcaatcc
     2941 ggaacatccg atgggcccaa tgaatttggc cgttataatt atacttttaa aaccggttta
     3001 cgagggaaac tattatccgg gacgagcacg atatcgctac ttttaccgta cgatgcgacg
     3061 tttactacgg gtacacccac tgtaagtacg gttacggtca atggtaccgc ggcagcggct
     3121 ctgcgtttgg gtctatcaac actcaatgaa gatactttgg aggtaacggt accggcgtca
     3181 gtcacgattg ggaaccaaac gtcagtaaca gtgatcattg attcgacatc cggattacgt
     3241 aatgcatcaa cagccagcgc acttacatac aaagtgttta cgagtgtcga aacgggtgtt
     3301 gtcggcggag atgcaacatt gccggtggag ttggcctttt ttgatgtggt acaaaatggt
     3361 aacaaagctg tattgaaatg gcgtactgaa agcgaaatca ataatgctta ctggcttgtt
     3421 gaacgcgctg aatcgaattt tattgaaaac gaggacgatc tacagggtaa gacctctcat
     3481 ttacaatata cgagagtggc agaaataacg gggcagggta ctaaaaatac cagtacggat
     3541 tatatattaa acgatgacaa ggttgaaatc ggtaaaacat atgtatatcg tttgatggat
     3601 gtgtcctatg atggagtttt atctatccat caggagaaaa aaatcataat cgaaacaccc
     3661 caagtgttcg atgtgttccc caactatccg aatccgttta acccgagtac attgattcgc
     3721 tttcaaatcc catcagagtc gcatgtcgta gtgactattt ataatttact tggacaagag
     3781 gttaaagaac tggttaacaa ggatcttccg gcaggaatgc atacgatgca atggaacggt
     3841 accaacaatc gaaatagtct ggtggcatcg ggcgtatact tataccgcgt aacggcaaca
     3901 agtttgcaga ataaatccaa atattcgcga acgcatcgca tgacactatt aaaataaaac
     3961 gtaaaaaccg atgtgctaca tcggtttttt tatttaaagc tattttactt ttacgcgatc
     4021 cggatgagct tttatataag ctttccagct tgaaacagaa gcgcgtggtg ttttgatacg
     4081 ttgagcacga caaatagcca aacctagggc atcggattca tcaaaactca tttctttagt
     4141 gatacccagt accattttaa ccatgtattg gacttgttct ttagaagcgt tgccgtttcc
     4201 gacgacggcc attttcatat ctttggggga gtactcgctg atatgaactg ctttatgtaa
     4261 cgcgccaagc atggcggcag cccgcgcatg gcctagtttt aatgcggatt ttacattttt
     4321 actgtaaaac acattttcta tggcgcattc gttcggtttg taagtatgtg ccaaatcaac
     4381 gacatggtcg tatatcaatt caagcttacg agctaatgtc tctttctctg gaagttcgat
     4441 cgtgccgctt gcaacccaat tgactttgtt acgctcccag gtgatgattc caaacccggt
     4501 ggtgttggta ccgggatcaa tgccaagtat gaccatcagt tccttgcgta ggtttgctgg
     4561 attcgctcta agttaacaaa cacaagttct ttgtgtgtaa gcgacttgat cgggtagtaa
     4621 tcgtattgtg tatagggttg gccgttcgta tcaatttcat ctcgaaacat tacaatttgt
     4681 ttgtcgtcaa caaaaaaatg ccattcttca tcacgtacca caggcggatt cgtagccgtt
     4741 ttgctatata cgtgaagcga taaatcgtct cgaaaagtga gttggatttc gcccggcgtt
     4801 tctggaaagg tggacactag tatccaagtg ccgaccaaat tgttttcaag tttagcatct
     4861 tcgctgttga ctaaaggctc tcggcaactg aggttcatta ccaaggccgc aaaaaaaagt
     4921 agcttcaaac tacggttcat taatttttcc cctttggcgt ttttcggata aagcgataca
     4981 gtatgaagta agcgtagtat atattttaag aatatctttg gaatgctttt ggatcgcttt
     5041 tttatgaagt cgaatggttt gtttgtcacc gcgcatggcc gggccggtta atgaagccgc
     5101 tgcaccatgt ttgcttatat tggctaacgt ctgttgttga ataggtaaaa aaagtttttc
     5161 taaaccgctt gattgaggat agatttgccg gagaagttcg gtggatgcat gtgataaagc
     5221 tactaaaaaa ttggatgcga tgacggccga ggcatgatac gttgcgcgat ctttatcgga
     5281 tatcataata gattttccac ccattatttt tactatatgt ttgacgatac tcaccggttt
     5341 gttttttccg tcgagtgcaa aatatgtttt aaatatttca ggcgatgtat ccattcgtgt
     5401 tacaaaagtt tgtaatgggt gcatagcggc cgtccaaaac tttacttctt tattttcaga
     5461 agttcgttga ttccaaattg ttgcgccata catgccggac gtgtggataa gtaacacgga
     5521 attatgaata aaacgaatct gcataacacg ttttgttatc gctttaagct gccggtcggg
     5581 tacggaaact atgattacat tacaagtacg taaatcttct attttattac tgaatatttt
     5641 acaatttatt tttttggcca aaacagcagc actatgagat gtagccgata caacgcttaa
     5701 tatcgggatt ttattccccg acaaagccaa cgcgatcgat gaacccacac gcccggctcc
     5761 taaaatgccg acacgccagt tgtttttttg cgataggttc attgatgaaa ttactgagca
     5821 ttatataaaa aaacaatctt gatttacgaa caggcatggt ttacattcgc atcattcatc
     5881 ggaggcgttt atttatgttc gatacctatt cttcgaaagt tgaattggct caaagtgatt
     5941 ttaatgaaat tgcagaacag tattttcaga actatttatc gtcatggtgt gatcataata
     6001 aaatagacga cgcatcacgc aaagccatgg tgaccgtact gtcggattat tctaatttct
     6061 tttttgacgc attaatgcaa tccaccatag agcaacatgc cgatagaact gaattgaaaa
     6121 attaatatgt ccggaagcgt tatacataat aaacactatt ccccggagga agcgcgaatt
     6181 ctcttacacg atatacgcgt caaaattaat acactcgttg cgctcaaaaa gcatctggat
     6241 gagatcaatt ataatatcta caaacacgaa tattttgggg gattaggtcc caatgggtcc
     6301 aagtttcatc cggaagaact catgacgttg gttgatatac tacgatcact tgaaaagcag
     6361 ggtgtcatca ttaagagtat cgatgaaggg cttattgatt ttccggcaat tcgagccaac
     6421 ggggaagaag tgtatttgtg ctggaaatta ggtgaagaag atatttttta ttggcatacg
     6481 atccgcgatg gttttcaagg gcgacgcccg ttatctgctt tttaatttaa caagtttatc
     6541 gcatctacaa atttacccgt tccttttgcc ttcgggtcta atcaaaggtt tggtatggcg
     6601 cgtgccggtt cgataatacg ctcgtcctcc ggaaaagata tttttacgcg gcgagataaa
     6661 gattttttcg aattttttgg gcaactcgaa tcggtagaaa ataagtttaa cgcctatctc
     6721 acgcggttca aatatcccaa gcgtgaaaaa tatcttattc gcaaagccta cgacttttca
     6781 tcactggctc acaaaggaca attgcgcaac gacggggcgc cctatatcat ccatccgatt
     6841 cgtgtggcgt ccatactttt gcatgaattg tcagttactt cggcggatat gatatgttct
     6901 gcgcttttgc acgatgtgat cgaagattgc ggcatcacgg aaaacgagat aaaaaataat
     6961 tttaatgagt cggtagcctc gatggttcgc acactaacca aaaatccgaa cctcagtaat
     7021 gctaagcgcg tatattttga agcgatcatg caggccgaag atcacatacg ccttgtaaag
     7081 ctttgtgacc gcttagataa ccttcgttca ctacggttaa tacgtaataa agccaaaaca
     7141 acccgctaca ttaaagaaac tgaagggcaa tatctgccca tagcggcgga gatcagcccg
     7201 tatctttttc gtgaattacg atttgaaata caatatcaac gaacacgtta ccgataaata
     7261 ctcatataaa cgaatttcac taaatatgga gttgcaatga aagaacgcgc aggacatctc
     7321 agcatcaaag gaaaacctct tacatggtta ggcgaagaaa tcaaagccgg gcaaaaggca
     7381 cccgattata gagtgcgtac ggggtttggc cccgaagcaa ccgttacgtt agctgattcc
     7441 aaaggtaaaa tccgaattat ctcggtcgcg ccttcactgg ataccggtgt atgtgaaatg
     7501 caagccattc ggttcaacca agaagtggaa aaattaggcg ccgatatcga agtgcatcag
     7561 atcacggttg atttgccgcc ggccatgaca cgcttttgta ataccaaatt aaacggcagt
     7621 gtgaagatca aaactttttc ggactatgcg gagcgctcgt tcggtcaggc gtatggtttt
     7681 ttgatcaaag aatggcaaac gttagggcgt ggtatcgtca tcatagataa aaacgatacc
     7741 gtcgcgtatg tggagtattg cccgaaaatc gaagagttgc ccaactttga tgtagcgcta
     7801 gccgcgttaa aaggattgta atcatctgtt tatatcataa aaaaacctcc gctacaacgg
     7861 aggttttttt atagccataa tcaatcacgc ttcaaataat taattattga aataacccgt
     7921 taataaactc ttcgatagtc cgcggacggg caggcgcttc ctcctgggcc aaaggggaag
     7981 ttgtggggtt tgcttttgtt tctaccatat caatagaaat aacctcatcg ggagtccact
     8041 ccggttctgt tttcacaggt gcactgcgaa atgcacgacg tgtgctcagc gatgaattga
     8101 aaaacgattc gatggtatgg ctcggcggtg atgcggtcgt agcgatgata tccgtaccta
     8161 aaggctgcaa aaaatccagc atagtaagaa tatctttact gaaaaagtca ttcgccaaag
     8221 ccggtgcggt ttgtactgac tttttagtcg tacgccgcat ggatgtaaat tgactgagga
     8281 gatgggccgc taggccggtc tcttcaaacg gttttgattt agcattcatt ccgttcaacc
     8341 ttcgaattct tttttgactt tttcaaacgc gtccagcggc gtggttttac ctgaaatgcg
     8401 tgctttgacc atcgcgatgg tttcctcatt atcgagcaaa aaggaacaat gctcgtaacc
     8461 ttcgtttttg caggtaatct cgatgcaagc caggggcata ttggatatgc gggaaaaaat
     8521 accggcaaaa aaaccggcgt ataatgcgca cgacgtgtga ggtttggaac catgcgcctg
     8581 cgactgcgat tgcaacattt ccacccataa cgaatggtat aaatcaacaa atagaaaatc
     8641 gtcgcgacgt ttaagttcaa aattgcccca acccaacgct gcaagatgat tggtaaaaag
     8701 acgatgaaaa tccggcatgg ggatttcacg aatggatttc gcatcagggt gcatggtcag
     8761 cgctagcgtt tcaattttgg cgtaaaccga gttgccccat tgtacgccgg tacggtagag
     8821 ggcatcctga tcgtgcgctg catacaggcg cgcgaaagac tgttgtaatg ctttgaggaa
     8881 aacggtatcg acggcaaaac tccgatttgt gttttgaacg aaaatttcag cgcttacggg
     8941 agaaaaagcc gtgtagtttt ccggcaatgt aaattcgtac gatgtagttt ccataatttc
     9001 cgtttggttg cagcggttaa tgggatatcg agtttaaccg caatggggtt tcagaaaatt
     9061 gaatggcgat ttccgatgct aaggcttgat aggcctgaaa agcgacggat tgcgcttcga
     9121 tttccgtcaa taccgaagta atattttcgt gacctgcaaa aagcaaattg ataacagggt
     9181 cgcgcggtat ggcctgttta aaaacgatat cctcttcaaa aaaatctcgt atttgttgcg
     9241 cgaccatttc ggaagccgga ctttgatgat cgtacatagt cagcaggata cccgccatgg
     9301 caagatcggg gttatgttcc tttttgatcg cgatgagttg gcgcagtaac tggggtaagg
     9361 tgcgcaggct gagcggttcg ctctggaccg gtatgatgac atagtccgaa gcggcgagtg
     9421 cgcctgcgga gacaaggccg atacccggag ggcaatcaat aagcaccgta tcgtagtgct
     9481 gagcggcttg tatgaccgct tcgcggaata aaccggtggc ccgcgtagca tcctcgtaaa
     9541 gctcgatgtc ggagctgcgt gcagaaatgc cgcaatcaat gacagaaaga ttttcgatca
     9601 tggacggttg agctgcttcg gcgatatcca tagccccgca gaagacctga tacaatccgt
     9661 ttcgcccctg atcctgatcg cgacgcatat cgtagtagcg atccagacaa aacgagatac
     9721 cgccttgtgg atcggcatca atgatcagtg tttttttatt taaccgtgaa aaagctatgc
     9781 cgaggctcaa ggctgtcgtg gttttgccga caccaccttt ttgactggct atgctgaaaa
     9841 tctggcccat gtattgatca gaggagatgt tgattatgat actgcgtcaa tatatcggtt
     9901 tcggtaagac cggcattgat ctgcgtgcga atatcttcga tcacttcggt tgtgctgaga
     9961 gcaaaccgcc attgttcagg tgtattggat aaaaatgtgc tgctaagcgc gcgaccggcg
    10021 ataccagaaa aaagggttga aaaaaaaccg gcaaaactcg tcgaggcata tatttgtttg
    10081 tgctcgtccg acgggcgcca tgggtttttc agatcaatga tgtaaaattt attcccttcc
    10141 agtatacgaa actggccaac gcctaaataa ctgaaattag aattgagatg ctcgacaaat
    10201 tcgtctttta gcagcgcttg aggttctgat gcatgctcac cgccggcctg tgccaattga
    10261 gttttcaacc gttccagaaa agaattaccc caatcagcgc caaactgata aaaacgttcg
    10321 cgtgtttgat caggcgttga attttcagaa aaaacagaaa atagagcttc aaaaaaatca
    10381 taatcggcga tgccgatacg tcgtgccgtc ggcgtaaaaa agtccgaaag agcaatggag
    10441 ccggttgcat ggtctatctg catgtgattt cgtatggccg cttcaatggg gttcgtaggc
    10501 atgagttcga tttattaccc gataatgtaa aacgcgttaa tgcggcaaac taattgattt
    10561 ttcgatggaa agcaacgcga aaatcttttg tatttttgag taaaattaat actttagaaa
    10621 aaagaaatta gtgcatgcaa gagttatcaa acgaaaataa tgttcatctt gtgaagcgtg
    10681 tggccagtgt tgtggtgacg atcatcatca tgctggcttt gcgttattct acattgataa
    10741 ctgaatcgga tccgtccgtt ttttcggtga cattgttggg tttaatgctc gtactttcgt
    10801 ttaatatcgg caaattactt tccagactta atcttcctac attatccatt tacctgataa
    10861 cggggatttt atgtgggccg tttgtacttg gttttttgag ttcttctacc attgataacc
    10921 tgaagtttat agatacgctt gcgctcgggc taatagcttt tatagcagga ggtgaattgc
    10981 ggttaaaaga attattaaga atgaagaaag tgttagttag catttcgtta atggaaacat
    11041 tgatggtttt tatattttgt gcagtcggtt ttattgtact aaccccatta atgcctttta
    11101 cacaatccca aacttttctt ttaaacagtc tcattgcatt gatcatggcc gcactgttag
    11161 tggccaattc gccggctgta acgataggaa tcatcggcga atataaaagt aagggactct
    11221 tgacggaaac aattttaggc tcggttattt ttaaagatat tattgttatt gtcttgtttg
    11281 caaccgttac aacctttgta atgatttcgc tcgattcgac cgcggagtgg agcatgatgg
    11341 ttttcataaa gacgatagga tatgaaatcg ggatgtcaat cgtggccgga ttgggtgttg
    11401 gatttcttgt gtatttgtac atacgattta tcggagaaca acccgtttta tttgtaatcg
    11461 gcgcggcatt tatgtcctac gaaatagcgc gccaatttca tctagaagtt cttttggtcg
    11521 gtgttagcgc tggttttgca gttcaaaatt ttaccaaaca aggggaggca ttagtcgagc
    11581 atgttgagga aagccttcca atcgtttatc ctattttttt tgcaatcgca ggagcgaaga
    11641 ttaatttgcc gttaatgctt gaaatgtggt atcttgctct gtttttagca gtgattcgaa
    11701 ctttaggaat ttattttggt gttcgaaaag gtgctcgtat ggcatcagca gctgaatctg
    11761 ttcaaaaata cgcatggatg ggctttgttt cgcaagcggg tgtagcatta gggttagcga
    11821 caatcgttaa ttacaaattc ccggagtggg gaggttattt acaaacaatt ttattgtctg
    11881 ttattggaat aaatcaacta gtgggaccaa ttttgcttca aaaagcgctt gataaagccg
    11941 gagaaataaa taaaccaatg cctcaaggtt ctattttaaa ttaatagaag aaaagattgt
    12001 tataaattat tatggctaaa tttgtcgtac atatcgccaa agaatattta aaattcagct
    12061 cggcacattt tactattttc agcgatcatg ctctggaaat gttgcatggg cataattatt
    12121 atgtgacttt agatgccgag tttcgtgaca gcgctaacgg tatcactata gattttaaac
    12181 atcaaaaaaa aatcatggaa gccttgtgtg acgaactgga tgaaaaagtt ttattaccat
    12241 cggaatcgcc atatttaaaa attgaaaaaa taacagataa ttttcgtgtt gtttttcatg
    12301 gtgaaggttt tactaaggaa tatcaatttc cgtgcgaaga tgtacaaatt cttccggtga
    12361 ataatatcag ttctgaaatg cttgccaaac aactatgtga gtctttttgc gaaaagctta
    12421 tgcagacatg gcaacacctt aagcctggta gtgatatttc tgaagtgtta cttgctgtac
    12481 atgttggcgt cgaagagacg cgtggccaat ctgtaaaata tgtatgggag tactagcctt
    12541 ggcggaacta gaattaaaag aatttgagaa gacgatttgt gctcgtcagc ttacaataga
    12601 cgattacgaa tccgtgacag aattgcaaat tcgatcattc ccgggtatga aaccatggtc
    12661 gtatgatcag tttgaaagtc agattagact atttgcggaa gggcagatag gtatcgaata
    12721 caacggatta cttgtcgcat cttcttcgag cctcattctt gattttgatc cttacaaaga
    12781 ctggcacagt tggaaggaaa tttctgacaa tgggtatatt cgcaatcata tcgtgaatgg
    12841 taatacgctg tatggcattg aaattatggt ggatccagga tatcgaggat tgcgccttgc
    12901 gcgtcgttta tatgaagcac gtaaatcgct ggcaagaaaa tataacctaa gaagcattat
    12961 tcttgggggg cgaatacctg gttatgataa atttgcggat cagatggatg tcaaatctta
    13021 tgtggaaaaa gtaagtagta aagttcttgt cgatccggtt ttgacaacgc aaatcgcaaa
    13081 tggatttgta ctcaaacgca ttatacccaa ttatctgatg tcggataccg aatcaagagg
    13141 ttatgctact tttttggaat ggaccaatct tgattatgtg cccgattttg caggtgagaa
    13201 acaaaagacg atcgtacgga tttctgccgt acagtatcaa atgcgtcata tccgtgattt
    13261 tgaagatttt gcaaaacaat gcgaatactt tgttgatgtg gcatcagatt acagatctga
    13321 tttcatactt tttccggaaa tgttcaccat gcagctgttg acatttctac ccaatctgcg
    13381 ccccgggctg gccgtacgga aattagcaga gtacacgccc aaatatcttg aattattcaa
    13441 ccacttgtct gtaaaatata atattaatat cattggcgga tcgcatttta cggtagaaaa
    13501 tgaagatttg tacaatatct cctatttatt tcgccgtgac ggaactatcg ggaaacagta
    13561 taaacttcac attacgccca gtgagcgcaa atggtggggt gtgaaagcgg gacataagtt
    13621 agaggttttt gatacggatt gcggtaaaat atcgattcag atatgttacg acattgagtt
    13681 ccccgaactg acgcgagtag cggttgaaaa aggagcccag attgtttttg ttcccttttg
    13741 tactgatgaa cgatatgcat acctgcgcgt acgctactgt gcacaagctc gatgtgttga
    13801 aaatcaaatt tatacggtga tcgccggcag tgtcgggaat ttgccgtatg tggaaaattt
    13861 agacatacat tatgcacaat cggctatttt tacgccgtcg gatataccgt ttacgcgcga
    13921 cgcgattcag gctgaagcaa cgccgaatat cgaaacggtc attattgatg aagtgaatct
    13981 tgaaacactt cgtcacaacc gacagtctgg aagtgtatta aactggcaag atcgtcgaac
    14041 agacttgttc aaagttaatt atcaaggatc gtaattactt cattatttaa atagttcatc
    14101 atatagagga attcatgaaa tccaagacga taaaaaagaa taaaccaatt gtgaaaaaga
    14161 atagcgtaaa aacgcaacct aagataagcg aatcaaaaaa ccaaacccca tacagagctt
    14221 tgggcttggc gcccgatgat ttactgcgta tgtacaggtt gatgaaactg acacgccagt
    14281 ttgatcaggg cattatacgt ctgtaccgtc agaataaaat gatcggcgga gcgtatagcg
    14341 gatggggtaa tgaagcgacg gccgtaggta gcgcgtatcc gcttttgccg cacgattatt
    14401 tgtacccgat gcatcgtgat atcggcgcac attttaccaa aggccagtca gccaaaattt
    14461 tgatgctcaa ccatcttgcc cgtgcagaag gcccgacacg cggtgtggat gggacgggac
    14521 attattatga taaaaatctc cgaatcgtcg gtaatatcag ccatttggcg gcaatgatac
    14581 ctcaagccgt cggtacggca ttggcgtccg taaagaaaaa agaaaatgcg gtcgtgatca
    14641 attatgtcgg tgacggcggt atgaacgtcg gtgaatttca cgaaggcctc aatatggccg
    14701 cggtatggaa attgcccttt atcctgatca tcgaaaataa tcagtacgcg tattccacgc
    14761 ccaatacatt acagtttgcc tgcgaaagtc ccgtggatcg tgcattaggg tatggcatac
    14821 ccggcgtcaa agtagatggt acggacattt tggaagtgta ccgtgtttgt aaggaagcat
    14881 ttgaacgggc gcgtcgcggt gaagggccga cgttgatcga atcggttacg atgcgtatgc
    14941 gcgggcacgc cgaacacgac ggccatgaat attatcctcc ggggattttg gaaaaatggg
    15001 agaagaaaga tccgatcatg cagtttgaga aatttcttaa atcggagaaa ttaatcaccg
    15061 ataaaatgat cgaagacatt gatacgtcaa ccaaacaaga aattgacgaa gcgatcgaat
    15121 atgccgacaa agcgccgtgg ccagagcctt cgcaagcggc gacgtgggtg tacgcggaat
    15181 aattaaatcc taatattaac attggtaaaa aatacttacg catgaccggt caaagtaaaa
    15241 catcgacaaa accatacgct cattcgacgg aactggatct gaaaaaatac gaaccgacca
    15301 aagagcagat gaaagaaatg tactatttta tcatgctcaa tcgcgcgttc gatgatcgta
    15361 tcgccaaact ttaccgccaa ggtaaaatca tcggagccgc atacggcagc cgcgggcagg
    15421 aagccaccag tgtgggttcg acatatgcgt tgggtccgga tgatatcgtc gggccgatta
    15481 ttcgtaatgc gggttccatc atcgttcgcg ggctaccggt caaaaatttc ttgtcaaatt
    15541 ttgtcggtcg ttcgaccact ccgacgcgtg gccgcgacgg caattcacac ttgggtgatt
    15601 tgaatatggg cgtcttcgct ccgatttcac atttggggag cctgatcagc aatatcgcag
    15661 gttgtgcact ggcattcaaa atgaaaaacg aaccgcgtgt ggcgctgact tacatcgggg
    15721 acggaggtac gtccacagcc gagttccatg aaggtatgaa tatggccgcc gtgatgaaat
    15781 taccttttat ttgtatcatc gaaaataata actgggcata ttcgacgccg acgcaaaccc
    15841 aagcgatgat caaagatttt gccatcaaag gcaaagctta cggcatggag gccatcgtcg
    15901 tggacggcaa tgatgttttt gaggtgtttc gtacgacacg gtacgcggca gagcgctgtc
    15961 gaaaaggcaa gggacctatt ttgatcgaag cgaaaaccat gcgcatgaaa ggtcacgcgg
    16021 agcacgatga tgcgttttat gttcccaaag aacaatttgc ggaatggcag aaaaaagatc
    16081 cgattgcgcg tgcggaaaaa tttcttctcg ataaaaaata catgaccgca acggaaaatg
    16141 aggccttaaa acaacgtgtg gcgaaagata tggaagaagc ggaagaattt gcacttaatg
    16201 cgccgtttcc agccatggac gaagcggcga ccggtgtata cgctgattaa ggaggagaaa
    16261 cgatgaaaaa attatttcta atatttgttt tgttcgttca ttcggcattt gctcaaacct
    16321 catttgccat tattcctttc acgggtactg ctgatgtagg agttaatgat cgcgaagctt
    16381 ttgttagcgg actctatcaa gggatgacga atagcggtcg ttaccgtatc gtagatcgca
    16441 acctgatcga acaagtattg aaagaacaaa attttcagtt gagtgactta acggagcaga
    16501 aaaaagttat cgaattcgga aagattgccg gcgccgaaaa aattataact ggtaaaatgt
    16561 tcagatattc ttccacacaa ccggcgatta cattttccgt aattgatgtt tcaaccagtc
    16621 aggttgaatt ttctaaagaa atcacatata acaattatac gttttcaaac ctaagcagtt
    16681 ttttggtatc tcagttgatt gacaagtatc cgctggtggg tagagtgctt ggtaaatccg
    16741 gagatgtgta tatcatcaat attggcctca atcagaatat aaaaaacgga acgcgcattt
    16801 ttgtggctag aaataatgta ctcaaaggcg acgacggcga aattttgatg gaagagtata
    16861 agcgcgtcgg tattttagag gtgacattag ctgacaaagc gcgttcgcaa tgcaagttac
    16921 gtagtttggt tgaaagcaat gttatcattc aaaaagacga tatgatttct ccggatccga
    16981 taccggagcg tccggcactt gttttggata aacctttttg gtctggttat tcaaaaggag
    17041 agctgctttt agatgatgat atgagtaaaa agcagtatct atctgtaact agcgctttgg
    17101 gcgaaaccta caaagatgga cggcttttta ttaatacgac acatctgaat tccggtcatg
    17161 gttactgtta ttatccggcg ccttttgatg ccgtgggtga ttttgtatgc gaagcagaaa
    17221 tcacgtttga aaaaaacact gaaaagtata atagggtgac ttttatcttt agaaatgtcg
    17281 gtgcgtatgg cgaaggaaat ggttattcgt tacccgttgt gtgaattaaa tgaaagcata
    17341 tttgataagg tttagatttt tttgattttg gtaattgaag aattgagaaa tcgtaagcgc
    17401 agtaatttta gcaaggatac gagctttaaa gccgtcaaag gacttggcat aatttcggtg
    17461 aatcataaat tgatcacaaa gctgagagaa aagagtttca atgcgtttac gtgttttgcg
    17521 aaggatactt ggttgtttaa catagttttt ctgattggaa cgcagaggag tttgcaggtt
    17581 gatacggaca gaggtaaata ggtcaatttg aatatcttct gagagataac ctttgtcgcc
    17641 aagaagaatg gcattagcca tatgttcttt cacatttttg aggtagtgaa tatcatgcac
    17701 ggaggctttg gagagatcaa aggaagtaaa gacgcctttt tgggtacata cgctatggag
    17761 tttgtagccg tgaaaatagg tttgctgggc ggcacaaaaa cctttatccg gagcggtttc
    17821 aaaggactct ttacagattc gggagcgatt agcgcgcgct aatttgcaga cgggcaaagg
    17881 catggtatcg atgataaata ccgattcgga ggaagaaatc ttttggagaa tcgtattccg
    17941 tagatggttg gtataggaga atagtttacg tcgacgtaca ttgtatccgg aacggtcgat
    18001 gagcgtggga aagactttcg gatattgtcg cagttgcata aacaagcgat tttcactatc
    18061 aatggagagg atttcgctcg tcaaactcag ggcaataatg gcggcatcgg ataatttggg
    18121 acgaggaccg ggtttaggga tatttccttg agccgtaagc atgtcggata aatgttcgtg
    18181 aaccattttt aaaacacgat caaaggtttg tttgaggttg tgcatgaact ttggtctcct
    18241 aaaaaagaat ttgaaattac tctttttaga aaagccaaat tcatgccaac ttcttttatt
    18301 aaatcataat taattcacac aacgggtt
//
LOCUS       DAOQGF010000064        18270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1422_length_18270_cov_6.931540, whole genome shotgun sequence.
ACCESSION   DAOQGF010000064 DAOQGF010000000
VERSION     DAOQGF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_18270_cov_6.931540"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1082)
                     /locus_tag="PK937_09810"
     CDS             complement(63..1082)
                     /locus_tag="PK937_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNH32907.1"
                     /translation="MYHVTLIPGDGIGPSIMEAAVEVVEATGVKFNWDTRLAGMAAVE
                     KFDSPIPFETLDSIRKNRVAFKGPLTTPVGGGYRSVNVAMRQEFELYANVRPAVSFEG
                     IETRYQNVNILMVRENTEGLYAGVEHSIKVDGKVIAAESTAIITRHESERCIRYAFEY
                     ARKNKRKRVTLVHKANILKATTGLFLEIGKEIAKEYPDVEFNDVIIDACAMKMVMDPT
                     QFDVFVTTNLFGDILSDLAAGLVGGLGLTPGANIGKSAAIFEAVHGSAPDIAGKNIAN
                     PTAVIMAGMMMLEYLGEFEAARRVETAVKNVIKEGKIVTPDIRKDTAYGTKEFGKEVA
                     RRVRG"
     gene            complement(1111..1851)
                     /locus_tag="PK937_09815"
     CDS             complement(1111..1851)
                     /locus_tag="PK937_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNH32908.1"
                     /translation="MDSRFTTVIMAAGKGTRMKSDLAKVLHPLHGRPMIHYVIALART
                     LGSDRVIAIIGHQKERVREALADEKIEFAVQEPQLGTGHAVMQTESLLQNDHGDVLVL
                     SGDVPVLTEATMRKLIRLHQSESAVASVLTATMPDPTGYGRVLRHEDGRVRKIVEHKD
                     ASDEERRINEINSGIYMFQSQSLFAALKKITNSNAQNEYYLPDVIKIFIDAGQKVCAY
                     PTDNFREISGINTVEQLREAEAIMQPQS"
     gene            complement(1858..2709)
                     /gene="panC"
                     /locus_tag="PK937_09820"
     CDS             complement(1858..2709)
                     /gene="panC"
                     /locus_tag="PK937_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNH32909.1"
                     /translation="MTIITSPSAMRAWTAQQKSENHRVGFVPTMGYLHQGHLSLVSLA
                     RTEGKCDRVVMSIFVNPTQFGPNEDFEKYPRDLERDRVMAETAGVDVLFFPSVDAMYS
                     TDATTMVDVGAMGTVMCGISRPTHFRGVTTVVAKLFSIVRPDTAVFGQKDAQQFFIIE
                     RMTRDLHMDVALIMAPIRREQDGLAMSSRNVYLNTDERHQALALNEALREAESLIRNG
                     ERNVDVIRTHMESIFARYPLVRKDYIAMVEAGSLAKREVIQGRTLIAVAAYLGKTRLI
                     DNIIITV"
     gene            complement(2706..3716)
                     /locus_tag="PK937_09825"
     CDS             complement(2706..3716)
                     /locus_tag="PK937_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNH32910.1"
                     /translation="MKKILLIHTGGTMGMKFGYPAPLPHEQFADEVFRHIPGVRDIAD
                     VDFFSSFSMDSSNLSPEHWVTLGRIIWDNMDAYDGFVIIHGTDTMSYTASALSYMLSN
                     LAKPVILTGSQRPLSAIRTDAKNNLINAIELATYPIPEVCVFFDYKLFRGNRSKKISI
                     DEFDAFVSPNFGTLADVGLHIEIHENLLRSPSDMLRFDTAFGRDVISIRLFPGMNAAL
                     LDGLAQTSAKVFILEAFGSGNVPTLENSVIPFIKKTTETGKMVAIASQCISGGIDLNL
                     YDCGKEALMAGAVSCGDMTSEAAIIKAMYLLGQFDGHVGRVRSNFNTSIAGEITESDS
                     NS"
     gene            3934..4125
                     /gene="rpmB"
                     /locus_tag="PK937_09830"
     CDS             3934..4125
                     /gene="rpmB"
                     /locus_tag="PK937_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNH32911.1"
                     /translation="MKRCDVCGKSPMYGNNVSHANNRTKRRWLPNLQEVRAKMNNGAV
                     KRVKVCTQCIKSGKVAKAA"
     gene            4369..5499
                     /locus_tag="PK937_09835"
     CDS             4369..5499
                     /locus_tag="PK937_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32912.1"
                     /translation="MYRRLFLLVWCAPILAFAQVFVPATRMPKTWINTLSLSSGFEYN
                     TWSPYKADELRYTIEGFQIGYLRGEIDHNVPMLPNLNFSWETNFGGKKQNELMQAQNS
                     TSKLEQGYEKLHTALEFGKKKIYNDAGQVINTMANWELTYTKETFYIAVSPKSSDLRY
                     AAYYSDAVYNFPQGTKYSMFTKFQEIGLTAYTGGESYFMMIFTGLFGGGSGSVQKLGK
                     DTETRFGFYYATFQKPYMVSQVLSGGTNNGESATVYNTRFKSYGIKEEIKFDGKGGYF
                     NFTQKLGLVTVNLRKGTELEDSESPLFFHYAIGLEGGLRIQMDRFHVLLGASGEYGFM
                     LGGTYNEEEENDTTGEEGKIKTTSFINNDLLIKYRGVIQYYF"
     gene            5510..5911
                     /locus_tag="PK937_09840"
     CDS             5510..5911
                     /locus_tag="PK937_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32913.1"
                     /translation="MIVRTAYMLIWVSMMTLTACDIGEMFSDEGTDDFLESQLGGGSG
                     GEGSATFYTQINYSGYIYIYVDNNYVGMLTQYYNSGNSPSCGANSGGAVTITNVSSGS
                     HNLSASDGSYSWSGSFYVSETHALKSRYNKG"
     gene            6038..6754
                     /locus_tag="PK937_09845"
     CDS             6038..6754
                     /locus_tag="PK937_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32914.1"
                     /translation="MRVSKKTWFNSLLILSITLGMACGDDKTSDNNVSVSFAGDIPPL
                     LTKGLKKSASHVTIDTAKILIKSIKLGGSGPSDSSDFKTGPYVLFITEGASLKTLSAA
                     NVPEGEYDQVKFEIHLPSQNESVPDPDFREGSGGNERYSAVIIGHYDGSRFVFKSKST
                     VNVQSDISPSVIVADSVGTANLTLGVSTSIWFTDGEVNLDPTNTNASNVAKIEQSIRN
                     SFYGFRDNNKDGDADVQIER"
     gene            6893..7624
                     /locus_tag="PK937_09850"
     CDS             6893..7624
                     /locus_tag="PK937_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32915.1"
                     /translation="MKAFRWYLFATVFAAFVVSCGDSSSKSSQKEGTTLSMKADMSIP
                     LAKSAAGDTVILDSAKVLLKRMKFESTTDEDSADFKIGPFIVRIDLTGGVTNITTSKI
                     PNGTYDKIKFKIHKPEDDEDIADPDFKTGTSDDERFSIVVFGKYNNVPFVYKSKKDVE
                     QKLDINPPLVVSDSTTSTNATLLVTPAVWFLKDGQYLNPNAESDRDDIDDNIKKSFKK
                     VFKDNDHDGDEHDDDDDHDDDHGND"
     gene            7791..10430
                     /gene="mutS"
                     /locus_tag="PK937_09855"
     CDS             7791..10430
                     /gene="mutS"
                     /locus_tag="PK937_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNH32916.1"
                     /translation="MPAPKDSTPLMRQYFEIKAKYRDTILLYRMGDFYETFDEDARTV
                     HKVLGITLTKRSNGKASEVALAGFPYHALDSYLPKLIRAGFRVAVCEQMEDPKAAKGI
                     VKRDVTEIVTPGTTLSEKILDQKTNNFLATVVTERRDDQTIFGLALADASTGEFFVGE
                     FREKEFLDQLNLFIPAEMVVPFSLYENMRMLLDRIKHRSVLTRQEDWLFSFQYAHDVL
                     MAHFKTHSLKGFGVQDFTVGLCAAGAGLHYLRETQRTDVSNVRHIGRLDDATYMTLDA
                     VTLRNLEILQATADGYTHGGLFSVLDHTETAMGGRMLKQWVVRPLKHTENIRKRLDAV
                     EELTAEHGARDKVRQTLSRIMDLERIIAKVCAGRVNPREIRALCESLKQIPELINNLS
                     NFKSDAIRGAVQAIHILDQLTDHIDNALVDEPSVQIKDGQVIREGYNEELDVLRRMAF
                     DGKSYIAGIQQRERERTGIASLKVQFNNVFGYYIEITHTHKDKVPPDYIRKQTMTNAE
                     RFITPELKEYEEKILTAEDKIIRIETELFDAIRKTVAQTAAEIQINAQRIAELDCYAS
                     LAEAAVQNKYVKPVVDDSAALIIKAGRHPVVERILPPDAPFVPNDLQMNEEEQIHLIT
                     GPNMAGKSCYLRQVGLIVLLAQVGSFVPAQSAEIGITDKIFTRVGASDNLLGGESTFL
                     VEMNETANILNNATSRSLILLDEIGRGTSTFDGLSIAWAITEYLHQHPAIRPKTLFAT
                     HYHELIELEELLPRVKNYNMMVKKYDDKIVFVRKIVRGGSDHSYGIEVAKLAGLPASV
                     ITRAREVMGNLESHNISAHKDKSELAGTSGDQSTQAQFTLFEDGTGLRLKTVLENLDV
                     NSLTPLDALNHLNKLKAILTEQP"
     gene            complement(10427..12541)
                     /gene="recG"
                     /locus_tag="PK937_09860"
     CDS             complement(10427..12541)
                     /gene="recG"
                     /locus_tag="PK937_09860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNH32917.1"
                     /translation="MQVNPHTEIKFLKGVGPKRAEALSGISIHTVWDLLHYYPRRYLD
                     RSKITSIRQARIHEEVTIIGQVENFALIRNHRGKGSRFRLILSDPTGSISLVWFRGAQ
                     YFEKAFEVGETLAVYGKIDYYNREIQIAHPEIDRLDADENVNFMHTGSIIPLYASSES
                     LKRMGFDSRGFRRLIFPVLDCASLVEDTLPASLLQNLSLAPLDKTLRHIHYPSTHEEL
                     ADAVRRIKFEELFYLQLMMAFRKSQITHAQIGIPFVNPGEKAKMLAEKLPFKLTDEQR
                     KVLKEIYADMRSDKPMHRLIQGDVGSGKTVVALLAIVSAVESGYQAAFMAPTEILAEQ
                     HYYVIQDYLWGMGIQAALIKGGQKKSERNKIIEDIRTRQVDIIVGTHAIFQEHVEFAK
                     LGLVVIDEQHRFGVMQRAEIRAKALDKGIIPDVLVMTATPIPRTLAMAMYGDLDISVI
                     GELPTGRKPIKTAIRREDARKKIYEFIRQEIRNGRQCYIVYPLIEESEKLDLKAATEN
                     FEHLQTDEFPDLKLGLLHGKMSSAEKQAVMADFKKKATDILVSTTVIEVGVNVPNATI
                     MVIEHAERFGLTQMHQLRGRVGRGADQSYCILMVGKHHLMEETEQRLRIMEETTDGFK
                     IAEEDLRLRGPGEFFGTKQSGMPELRLANIVEDRELMALAKEAAFTIVNLDPQLRSAT
                     HKTVRKKFLEAYRDRMDLSQIG"
     gene            12636..13040
                     /locus_tag="PK937_09865"
     CDS             12636..13040
                     /locus_tag="PK937_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32918.1"
                     /translation="MMMSTEVSEIKKQHTVLIVDDEDEVRDMLRRILKLSSYRVLEAQ
                     NAQEAMEIAVREKPDVITMDIMMPDKDGITLCEEIKANPQTKHIPVVMVTVVGQRQRA
                     MTAGADYFMNKFFTIDELVSVIDKLIADKQTA"
     gene            complement(13047..14186)
                     /locus_tag="PK937_09870"
     CDS             complement(13047..14186)
                     /locus_tag="PK937_09870"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HNH32919.1"
                     /translation="MLYLPDDYIQRLGDFLQLHLDEPIPRTLKPFAEAITEISEGLKK
                     KNKRSEFIRRIYLDQKVFRDAYLLYYGSINFPKMHIPLAEIQKSDFFNQKKNLRVLDA
                     GSGTGSMLLGLAYWLKNETTLTAELTASDYASSSLDTLSAFYKALALPYPLRTQILNL
                     NHAVPSENKFDLITAGNVLNELEPDAESKILPWLESALTQDGFVIWIEPALMDLSRRL
                     LIFRDRLLKEGWFLYSPCFTNKPCPALTEETDWCHHDVPWQRPEFIRVLDGMTGHIRQ
                     SLKFTYIVMGKRDIHLSDVTIGNRDYLSTVRVVSERFEEKGRIRVFGCNDRGRCAYSM
                     NKRDKSDDNLAFKKIERYDVVQLTETEEKSNYVGIGKNTKVRIVE"
     gene            complement(14260..15534)
                     /gene="serS"
                     /locus_tag="PK937_09875"
     CDS             complement(14260..15534)
                     /gene="serS"
                     /locus_tag="PK937_09875"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNH32920.1"
                     /translation="MLDLKFIRDHTERVKTAAKEKKFEADVDAVIRWDDERKVVIRKV
                     EDLKARRNKASEEVAKLKKAGQNADAVIAETKSVGEEIKALDEQLKVIEKNLRDVLLT
                     IPNVPHPSAPQGTSPEDNQTIFTWGTMPSADFELKPHWELAEKHNLIDFATGVKIAGA
                     GFPVYRGAGAKLQRALINFFLDSAADNGYTEVQPPIVVNTDSATGTGQLPDKEDQMYV
                     VTRDEFFLIPTAEVPVTNIHREMTLKAEQLPIKYAAYTPCFRREAGSYGKDVRGLNRL
                     HQFDKVELVKFVHPDTSYDELESLRMDAERLLQALQLPYRTLLMCRGDMGFTQTKKYD
                     LEVWAAGQKRWLEVSSCSNFESFQARRMNIRYKDNQTGKPEFVHTLNGSGLALPRIVA
                     AIMENYQTDEGTIMVPQVLRKYMGMDIISATT"
     gene            15736..17652
                     /locus_tag="PK937_09880"
     CDS             15736..17652
                     /locus_tag="PK937_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH32921.1"
                     /translation="MYKTMSVLIVMGLLWAACRPSALPPLPKVYDPHSNARAEQAYVT
                     HMALDVRVDFDNKQISGRATFDVVVSDKNTLMLTLDTRGLTIDSVKLDQGSEIANYVL
                     DTNIPNSDYANDSSAIQISSAQATMGQALRISVAPTTKKAHVYYHTGHEADALQWLAP
                     AQTEGQKKPFLFTQSQPNLARTWFPCQDSPGIRMTYEARVQVPPGLMAVMSAESDTVL
                     HADGIYHFRMTHAIPSYLVALAVGDFGFRPLGPRSGVYAEHTMLARAAAEFTDVEAMI
                     AAAEKLYGPYLWGRYDLIVLPPSFPYGGMENPMMTFATPTIVAGDRSLVSLVAHELAH
                     SWSGNLVTNATWGDIWINEGFTTYFERRIDEAVFGRSFSEMEAHLGHQDMQAALKELG
                     TESPLTKLRLDLNDLDPDAGHSGIIYDKGYHFLRFMEEQIGRDRWDAFLRKYFATFAF
                     QSMTSERLMNYMRTELIRGDRALEEKLQLDQWILGTGLPSVCPVPQSDAFTKVEEEVK
                     KWKSGVPAKQLLTSSWQTQQWMHFLRLLPENMNTAQLTSLDEAFSFTRSGNAEIQFEW
                     FQHVITHKYTPAYARLEHYLTHIGRRKLVFPLYRKMASDPEMRIMAKRIYAVARRGYH
                     PVTYGSIDEILLKK"
     gene            complement(17663..>18270)
                     /locus_tag="PK937_09885"
     CDS             complement(17663..>18270)
                     /locus_tag="PK937_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH32922.1"
                     /translation="LIDTGRLMGSEAFGLSKLDHAVNAAARMTQVCIAHGDAVGLMLF
                     GSAVHSFLLPKKNTSQRALIAESLYTARLERTESDYTTAFQWLQRRQRHRALVLVFTE
                     IVDRYSSQILLGSMASLYPKHLPVFILMKDKALTEVLQRPVMHSDSAFEKGVTAELLR
                     EREEAIAFARSRGALVIDAMPDHFHSAVINLYLDIKARGRL"
BASE COUNT         4851 a         4062 c         4425 g         4932 t
ORIGIN      
        1 gaaaaggttt attcttttcc ggtttgtttt ttttgagagc agaaaatatc aacgcataag
       61 cgctacccgc gcacgcgtcg cgccacttct tttccaaatt ctttcgtgcc gtatgccgta
      121 tctttgcgaa tgtccggcgt gacgattttt ccttctttga tcacgttttt gacggccgtt
      181 tccacgcggc gtgcggcctc aaattcgccc agatattcca gcatcatcat accggccatg
      241 atcacggccg tcggattggc gatatttttt ccggcgatgt ccggagcgct accgtgcacg
      301 gcttcaaaga tggcggcgct ttttccgata ttggcaccgg gggtcaggcc cagtccgcct
      361 acgaggcctg cggcgaggtc ggagagaata tcaccgaaaa ggttggtcgt cacgaacaca
      421 tcgaattgcg tcggatccat caccattttc atcgcgcacg cgtcaatgat cacatcatta
      481 aattcgacgt cgggatattc tttggcgatt tcttttccga tttcaagaaa taaacccgtc
      541 gttgctttga ggatgttggc tttatgaacg agcgtgacac gcttgcgttt gttttttcgt
      601 gcgtattcga atgcataacg aatacaacgc tccgattcgt gacgggtaat aatagccgta
      661 ctttctgcgg caatcacttt gccgtccacc ttgatggagt gctcgacacc ggcatagaga
      721 ccttcggtgt tttcacgcac catgaggata ttgacattct gatagcgggt ttcaatgcct
      781 tcgaaagata ctgccgggcg aacgttggcg tagagttcaa attcctgacg catagccaca
      841 ttgacgctgc gataaccgcc accgacgggt gtggtgagcg gacctttgaa agcaacgcga
      901 tttttgcgaa tggaatccag cgtttcaaac ggaatgggcg aatcaaattt ttctacggcc
      961 gccatgccgg caaggcgcgt atcccagtta aatttaacgc ccgtcgcttc gacgacttcg
     1021 acggcggctt ccatgatcga cgggccgatg ccatcgcccg gaataagtgt cacatgatac
     1081 atttataatt ctcctgattt atggtaggtt ttaagattgg ggctgcatga tcgcttcggc
     1141 ttcacgcagt tgttcgacgg tgttaatccc ggaaatttca cgaaaattat ccgtcggata
     1201 agcgcagact ttttgccctg catcaataaa aattttgatt acatccggaa gatagtattc
     1261 gttttgtgca ttactattgg tgattttttt gagcgcggca aagagcgact gcgattggaa
     1321 catatatata ccggagttga tttcgttgat gcggcgctct tcgtccgatg catctttgtg
     1381 ttcgacgatt ttacgaacgc gaccatcttc gtgacggagc acacgtccgt aacccgtcgg
     1441 atcgggcatg gtcgcggtga gcacggaagc caccgcgctt tcggattggt gcaatcggat
     1501 cagttttcgc atcgtcgctt cggtcaaaac agggacatca ccggacaaaa ctaaaacatc
     1561 gccgtgatca ttttgcagta acgattctgt ttgcatcacg gcatgtcccg taccaagttg
     1621 cggctcctgc accgcaaatt cgatcttttc atccgcgagc gcttcgcgca cccgttcttt
     1681 ttgatgtccg atgatggcga tcacgcgatc ggagcccaag gtacgcgcta aagcgatcac
     1741 ataatgaatc atcggccgac cgtgcaaggg atgcaacact ttggcaagat cggatttcat
     1801 gcgtgtgcct tttcccgcgg ccataataac ggttgtaaat cggctgtcca taactcctta
     1861 tacggtaata atgatattat caatcagtct tgttttacca aggtacgcgg ccacggcgat
     1921 caatgtacga ccttggataa cctcacgttt agcgagcgac cccgcctcga ccatggcgat
     1981 ataatctttt cgcaccagag gataacgtgc aaaaatggat tccatatgcg tacggataac
     2041 atccacattt ctttcaccgt tacgaatcaa ggattcggct tcgcgtaagg cttcattcag
     2101 agccaaagcc tggtggcgtt catccgtgtt gaggtaaaca ttgcggctgc tcatcgcaag
     2161 gccgtcttgt tcacggcgta ttggcgccat gatcagagcg acatccatgt ggagatcacg
     2221 cgtcattcgc tcaatgataa aaaattgttg tgcgtctttc tgaccaaaga cggccgtatc
     2281 gggtcgtacg atcgaaaaaa gttttgctac aacggtcgtc actccgcgaa aatgagtagg
     2341 gcgagaaatg ccgcacataa ctgtacccat cgcgccgaca tccaccatcg tcgtcgcgtc
     2401 agtggaatac atagcatcga ccgatggaaa aaacaaaaca tccacgccgg ccgtttcggc
     2461 catcacccgg tcgcgttcta gatcgcgcgg gtatttttca aaatcttcgt tgggaccaaa
     2521 ttgggtagga ttgacaaaaa tactcatgac cacgcgatcg cattttcctt cggtgcgtgc
     2581 cagcgagacc aaacttaaat gcccttggtg cagataaccc atcgtcggca caaaaccgac
     2641 gcggtgattt tctgattttt gttgagctgt ccatgcgcgc atggccgacg gtgatgtaat
     2701 gatcgtcatg agttggaatc ggattccgtg atttcaccgg caatggacgt attaaaatta
     2761 ctccgtacac gtccgacatg accgtcaaat tggccgagca gatacattgc tttgataata
     2821 gcggcttccg atgtcatatc accgcacgaa acggccccgg ccatcaacgc ttctttaccg
     2881 caatcataaa gattgagatc tatgccgccg ctgatgcatt gacttgcgat ggctaccatt
     2941 ttaccggttt cggttgtttt tttaataaag gggatgacgg aattttcgag cgtaggtacg
     3001 ttaccggaac caaaggcttc gagaataaat actttcgctg atgtttgagc caatccgtcc
     3061 aaaagtgctg cattcatacc cggaaagagg cggatactga tcacgtctcg gccaaaagcc
     3121 gtatcaaatc gcaacatatc tgaaggtgaa cgtaaaaggt tttcatgaat ttctatatga
     3181 aggcctacgt ccgccaacgt accaaagttc ggagaaacga aagcatcgaa ttcatcaatt
     3241 gaaatttttt tagatcggtt accgcgaaaa agtttgtaat caaaaaaaac gcatacttcg
     3301 gggatagggt aggttgccaa ttcaatcgca ttgatcagat tatttttagc gtccgtgcga
     3361 atggcggata acgggcgctg ggatcccgtg agtatcacgg gtttggcaag attggacagc
     3421 atataggaca aagcgctggc cgtataactc atggtatccg tgccgtgaat aatgacgaac
     3481 ccgtcgtacg cgtccatgtt gtcccaaata atgcgaccga gcgtcaccca atgttccggc
     3541 gaaagattgg aactgtccat gctaaaggag ctgaaaaaat ccacatcagc gatgtcccgt
     3601 acgccgggga tatgccgaaa tacttcatcc gcaaattgtt cgtgcggcaa cggagcggga
     3661 tatccgaatt tcatccccat cgttccgccg gtatgtatta ataatatctt tttcataaaa
     3721 gcccgcgaat ctaagtgaaa tcggagactt tggcaaacga atttgagtat gtgatccaaa
     3781 aaaagcggtt agcccaagct gccgggttcg gcggaaaaag gtatataaaa aaatattaag
     3841 tttttccttg catttacttg ccattttgta tagtatcgct gcgcttaaag aagaaattgt
     3901 ttttaatatg attgagccat aaaggagcga gtcatgaaac gttgtgatgt gtgcggcaaa
     3961 agcccgatgt acggcaacaa tgtgagccat gccaataacc gtaccaaacg ccgttggctt
     4021 ccgaatcttc aggaagtacg cgctaaaatg aataacggtg ctgttaaacg cgttaaagtt
     4081 tgcacccaat gcatcaaatc aggaaaagta gctaaagcag cgtaattttc tcgatttcgg
     4141 gtttatccca aaaaatgtaa aagcgtcgtc cccatcacgg cgctttttta tttttattaa
     4201 aaaaaaacgt ttcatcgttt agtttcgtca cgttcattat caagttttca cagtaatcct
     4261 ttcatttaag atttacttca ttgtgccgtc atacccggtt cggtttgccg gtgcgaaaaa
     4321 tcgtctgtca aaaacataaa ttatcaattc aatcgaaggg agtgcctgat gtaccgacgg
     4381 ctctttttgt tggtgtggtg tgcgccgatt ttggctttcg cacaagtttt tgtaccggca
     4441 acgcgtatgc ccaaaacatg gatcaatacg ctatcgctat caagcgggtt tgaatataat
     4501 acgtggagcc catataaagc ggacgaactt cgttatacga ttgaaggctt tcaaatcgga
     4561 tacttgcgcg gcgagataga tcataatgtg ccgatgctgc cgaatctgaa tttttcgtgg
     4621 gaaaccaatt ttggcggaaa aaaacaaaat gagttgatgc aggcccagaa tagcacatcc
     4681 aagctggagc aagggtatga gaaattgcac acggcattgg aatttggaaa aaagaaaatc
     4741 tacaacgatg ccggccaagt tatcaataca atggctaatt gggagttgac ctatactaaa
     4801 gaaacatttt acatagctgt cagccccaag tcgtcggatt tgaggtatgc ggcgtattac
     4861 tccgatgctg tttataattt tccacagggt actaagtatt cgatgtttac caaatttcag
     4921 gaaattggtt tgacggcgta caccggcgga gaatcgtatt ttatgatgat atttaccggt
     4981 ctctttggcg gcggctccgg cagtgtgcag aaattgggta aagacacgga gacgcgtttt
     5041 ggtttttatt acgctacatt tcaaaagccg tatatggttt ctcaagttct ttccgggggt
     5101 acgaataacg gagaatcggc gacggtgtat aatacgcggt ttaagtcgta tggtattaaa
     5161 gaagaaataa agtttgacgg caaagggggg tattttaatt tcacgcaaaa attgggactg
     5221 gtgacagtca atctccgtaa gggaacggaa ttggaagatt cggagagtcc gttgtttttc
     5281 cattatgcga tcggtttgga aggcgggcta cgcatacaga tggatcgttt tcacgtgttg
     5341 ttgggtgcgt cgggggaata cgggttcatg cttggcggta cgtacaatga agaagaggaa
     5401 aatgatacca ccggagaaga aggcaaaata aaaacgacaa gttttattaa taacgatctt
     5461 ctgatcaaat accgcggcgt aatacaatat tatttctaaa ggatcatgta tgatagtacg
     5521 cacagcatat atgttgatat gggttagcat gatgacgcta acagcgtgtg atataggtga
     5581 gatgttttcg gatgaaggaa cggatgattt tttagaatcg cagcttggtg gcggttccgg
     5641 cggagagggt tcggctacat tttatacgca gatcaattat tcaggttata tctatattta
     5701 tgtggacaat aattatgttg gtatgttgac ccaatattat aattcaggaa attcaccgtc
     5761 ttgtggtgcg aattccggtg gcgcagtgac aatcaccaat gtcagttcgg gatcgcacaa
     5821 cttgtcagcg tcggatggct cttactcttg gtcaggaagt ttttatgttt ccgaaacaca
     5881 tgctctaaaa tcaagataca ataaaggctg attaagtatt tttacgtaat ttctccatcg
     5941 tgtgatattc atcacactta gttttttata aagtaatact tttttgtagt tactcattat
     6001 taccaccaat atgatatacg ataaaaagga gagtgtcatg cgcgtgtcga agaaaacatg
     6061 gtttaactca ttgttgattc tctcaattac tttaggtatg gcttgtggcg atgataagac
     6121 ttcagataat aatgtatctg taagttttgc gggtgatata ccgccgctac tgactaaagg
     6181 attaaaaaaa tccgcttcgc atgttacaat cgacacggcc aaaattctta tcaaatccat
     6241 caaattaggc ggttcggggc ctagcgattc gtctgatttt aaaaccggtc cttatgttct
     6301 ttttattacc gaaggcgctt cgcttaaaac actaagcgct gccaatgtac cggaaggaga
     6361 atacgatcag gtcaaatttg aaattcacct gccttcacaa aatgaatcgg ttccggatcc
     6421 ggattttcgc gaaggttcag gaggaaacga gcggtattcc gcagtgatta tcggtcacta
     6481 cgatggaagc cggtttgttt ttaaatcaaa aagcacagtc aatgtacagt ccgatatttc
     6541 accttccgtg attgttgccg atagcgtggg cacagctaat ttgacgttgg gagtgagtac
     6601 ttcaatttgg tttaccgacg gtgaagtcaa tcttgatcct acgaatacca acgccagcaa
     6661 tgtggcaaag atcgaacaaa gtatccgtaa ttcgttttat ggatttcgag ataacaataa
     6721 ggacggtgat gccgacgtac agattgagag gtagaatttg tctgctgtat tgctagcgca
     6781 gcattcgtat tattatgatg cattgtaagt aagtatgttg ataggatcgg gggtttacct
     6841 gtttacgtgc aggtgaatgt atttcacgtt attaaaatga aaaggagttc gtatgaaagc
     6901 gtttcgttgg tacttgttcg ccacagtgtt tgcagcgttt gttgtttcgt gtggtgactc
     6961 ttcatcgaaa tcgtcacaaa aagagggaac tacgttgagc atgaaagccg acatgagtat
     7021 tcctttagcc aaatcggccg ccggtgacac agtgatatta gattcggcga aagttctgtt
     7081 aaaacgtatg aagtttgaaa gcactacgga tgaagattcc gctgatttta aaattggacc
     7141 gtttatcgtg cgtattgatt tgacgggtgg tgtaaccaat attaccacaa gtaaaatccc
     7201 taatggaacg tacgataaaa tcaaattcaa aattcacaaa cccgaagacg atgaagacat
     7261 cgccgatcct gattttaaga caggaacatc ggatgatgag cgcttttcga ttgtggtttt
     7321 cggtaaatat aataatgttc ctttcgtcta taaaagcaaa aaagacgtag aacaaaaatt
     7381 agacattaat ccgccgctcg tggtttccga cagtacgacg agtaccaatg ccacattgct
     7441 tgtgacgccg gcggtttggt ttttgaaaga cggccaatac ttgaatccga acgccgaaag
     7501 tgatcgtgat gatattgatg acaatatcaa aaagagcttt aaaaaagtgt ttaaagataa
     7561 cgatcatgac ggcgatgaac atgatgatga cgatgatcat gatgacgatc acggcaatga
     7621 ttagtaattg ttgatcgata ttatttcaga tctaagcgag tattgtaaac agtactcgct
     7681 ttttgtttta ttgcacagat ttcagcagaa gcgacggctt atgcttttat attttgaata
     7741 aatccgtctt tgtagctata cttgccttcc aaaaaataga gaaacaggta atgcccgctc
     7801 ccaaagattc gacgccgctc atgcggcaat attttgagat caaagccaaa tatcgcgata
     7861 cgattctttt gtatcgtatg ggagactttt acgaaacgtt tgacgaagat gcgcgtacag
     7921 tgcataaagt tctgggtatc actctgacca aacgttccaa cggcaaagcg tccgaagtag
     7981 cgctggcagg ttttccttat catgcgctgg atagttatct gcctaaatta atacgtgcag
     8041 gatttcgggt agccgtgtgc gaacaaatgg aagatccgaa agcggccaaa ggcatagtca
     8101 aacgtgatgt gacggaaatc gtcacaccgg gcacaacgct ttccgaaaaa atattagacc
     8161 aaaagaccaa taattttctc gcgacggtcg tcaccgaacg tcgcgacgac caaacaatat
     8221 tcggtttggc gttagctgac gcttcgacgg gggagttttt tgtcggagaa tttcgtgaaa
     8281 aggaatttct ggatcagctt aatttgttta ttccggcaga aatggttgtc cctttttctc
     8341 tgtatgaaaa catgcgcatg ttgctcgacc gcatcaaaca tcgtagtgtt ctcacgcgtc
     8401 aggaagattg gttattttct tttcaatatg cgcacgacgt tttgatggcg cattttaaga
     8461 cgcattcgct caaagggttc ggcgtgcagg attttacggt cggcctctgt gccgccggag
     8521 ccggactgca ttacctgcgt gaaactcagc gtaccgatgt cagtaatgtt cgtcacattg
     8581 gtcgattgga tgatgcaacc tacatgacct tggatgcggt cactctgcgt aatttggaaa
     8641 tactgcaagc taccgcagac ggttacacgc acggagggct attttccgta ctggatcata
     8701 ccgaaacggc catgggtggc cgcatgctca aacaatgggt agtgcgtcct ctgaaacata
     8761 ccgaaaatat ccggaaaaga ttggatgcgg tagaagaatt gaccgccgag catggcgcac
     8821 gggacaaagt gcgacaaacg ttgtcgcgca tcatggacct cgaaagaatc atcgccaagg
     8881 tgtgtgccgg gcgcgtcaat ccgcgcgaaa ttcgtgcgtt gtgcgaatcg ctcaagcaga
     8941 ttcccgaatt gataaataac ctatccaatt ttaaatccga cgcgatacgc ggtgcagtgc
     9001 aggcgataca tattttagac caactgacgg atcatattga taatgcactg gtggacgaac
     9061 cctcggtaca aatcaaagac ggacaggtta ttcgtgaagg gtacaatgaa gaattagacg
     9121 tgttgcgccg gatggctttt gacgggaaaa gttatatagc cggtatccag caacgcgaac
     9181 gcgaacgtac agggatcgca tctttgaaag tacagtttaa taatgtcttc ggttattaca
     9241 tagaaatcac ccacacgcat aaagacaaag tgccgccgga ttatatccgt aagcaaacga
     9301 tgaccaatgc ggaacgattt atcacgccgg agctgaaaga atacgaagaa aaaattctta
     9361 ccgcggaaga taaaataatt cgcatcgaaa cggaattatt tgatgcaata cgcaaaactg
     9421 tggcgcaaac ggcagcagaa attcaaatca atgcacaacg gattgcagag ctggattgtt
     9481 atgcttcact ggccgaagcg gcggtacaaa ataaatatgt caagcctgtc gttgacgata
     9541 gcgcagcact tatcatcaaa gcggggcgtc atcctgtagt cgaacgcatt cttcccccgg
     9601 atgcgccttt tgttcccaac gatttgcaga tgaatgaaga agagcaaatt catctgataa
     9661 ccggccctaa catggcggga aaaagctgtt atctgagaca agtaggattg atcgtattac
     9721 tggcgcaggt tggaagtttt gtgccggcac agtcggctga gatcggcatt acggataaaa
     9781 tctttacgcg cgtcggggct tcggataatt tgctcggcgg tgaaagtacg ttccttgtcg
     9841 aaatgaatga aacggcgaat atcctgaata atgccacgtc gcgcagtctt attcttttag
     9901 atgaaatagg tcgcggtaca agcacttttg acgggttgtc cattgcttgg gccattaccg
     9961 aatatttgca tcaacacccg gcgatacgtc caaaaacctt gtttgcaacc cattaccatg
    10021 aattgataga actcgaagag cttctcccgc gcgtaaaaaa ttacaatatg atggtaaaaa
    10081 aatacgacga taaaattgtt ttcgtgcgta aaatcgtccg cggcggaagt gatcattcgt
    10141 atggtattga ggtagccaaa ctcgcggggt tgccggcatc ggtgattacc cgtgcacgag
    10201 aagtcatggg taatttggaa tcgcacaaca tttcggcgca caaagataaa tccgaactcg
    10261 cgggcacatc cggcgatcag agtacgcagg ctcaatttac gttatttgaa gatggcacgg
    10321 gcttgcgttt aaaaaccgta ttagaaaatt tggatgttaa cagtctcacg cccctggacg
    10381 cgctcaatca tctgaacaaa ctcaaagcca ttcttactga acaaccttag ccgatctgac
    10441 tcaaatccat ccgatcacgg tacgcctcca aaaatttttt ccgaacggtt ttatgggtag
    10501 cgctacgcag ttgcggatca agatttacta tcgtaaaggc ggcctctttg gcaagcgcca
    10561 tcaattcgcg atcttccaca atattggcta aacgcagttc cggcatgccg gattgtttgg
    10621 ttccaaaaaa ttcacccggt cctcgcagtc gcaaatcttc ttccgcgatt ttaaatccat
    10681 ctgtcgtttc ttccatgata cgcagtcgct gttcggtttc ctccatcagg tgatgtttgc
    10741 cgaccatcag tatgcagtac gattgatcgg cgccgcgacc tacacgtccg cgcaactggt
    10801 gcatttgggt aagtccaaaa cgttcggcat gttcgatgac catgatcgtc gcgttgggaa
    10861 cgttgacacc gacttcgatc accgtggtac tcacgagtat gtccgtagct ttctttttga
    10921 agtcggccat gacggcttgc ttttcggcgc tgctcatttt gccgtgcagc aatcccaatt
    10981 tcaggtcggg aaattcgtcg gtttgcaagt gttcaaaatt ttcggtggcc gccttcaaat
    11041 ctaatttttc tgattcttcg atgagcggat agacaatata acattgtcgc ccgttgcgga
    11101 tttcctggcg tataaattca taaatttttt ttcgcgcatc ttcgcgacga atcgccgttt
    11161 tgatgggttt gcgtccggtg ggcagttcac cgatcacgga aatgtccaga tcgccgtaca
    11221 ttgccatcgc caaagtgcgg ggaatcggtg tggccgtcat gaccagcaca tcggggataa
    11281 tgcctttatc caaagctttg gcacgaattt ccgctcgttg catgacaccg aaacggtgtt
    11341 gctcatcaat cacgaccaat cctaatttgg caaactccac atgttcctga aaaatcgcat
    11401 gcgtaccgac gatgatatcg acctgacgcg ttcggatgtc ttcgatgatt ttattgcgct
    11461 ccgatttttt ctgtccgcct ttgatcagcg ccgcctggat acccataccc catagataat
    11521 cctgaatgac atagtagtgt tgctcggcga gaatttccgt cggcgccata aaagcggcct
    11581 gataaccgct ttcgacggcc gatacgatgg ccaataacgc cacgacggtt ttaccactgc
    11641 cgacgtcacc ttggatcaat cggtgcatcg gtttgtcgga gcgcatatcg gcgtaaattt
    11701 ccttgagcac tttgcgctgt tcgtcggtga gtttaaaagg taatttttcg gcgagcattt
    11761 tggctttttc gccgggatta acaaaaggaa tgccaatttg cgcatgggtg atctggcttt
    11821 tgcggaaagc catcatgagt tgaaggtaaa ataattcctc aaattttata cgacggacag
    11881 cgtcagcaag ctcttcgtgc gtggaaggat aatgaatatg gcgtaatgtt ttatccagcg
    11941 gggcaaggga taaattttga aggagcgatg ccggtaacgt gtcttccaca agggatgcac
    12001 agtccagtac gggaaaaatc aatcgccgaa aaccgcggct gtcaaaaccc atacgtttga
    12061 gtgattcgga tgaggcgtac agcgggataa tcgagcctgt atgcatgaag ttaacatttt
    12121 catcggcgtc taagcgatca atttccggat gcgcgatttg tatttcccga ttgtaatagt
    12181 caatcttgcc gtacaccgct aatgtttcgc cgacttcaaa tgctttttcg aaatattgtg
    12241 ctccgcgaaa ccataccaag ctgatggagc cggtcggatc gctaagaata agccgaaaac
    12301 gcgaaccttt accgcggtga ttgcgaatca gggcaaaatt ttccacttgt ccgatgatgg
    12361 tcacttcctc gtgaatgcgc gcctgacgta tactggtgat tttgctgcgg tccagatacc
    12421 tgcgcggata ataatggagt aagtcccata cggtatgtat actgataccg gacaatgctt
    12481 ccgcacgttt ggggccgacg cctttgagaa attttatttc agtatgggga tttacctgca
    12541 tgcgtataac ggtattatga tcgtatattt tcgatgaaac acattgctta cgaggccgcg
    12601 gcttcgtatt ttaacccgta aattgaaaga aaatcatgat gatgtcaacc gaagtctccg
    12661 aaatcaaaaa acagcatacg gttttgatcg ttgacgatga agacgaagtg cgcgatatgt
    12721 tacggcgcat attaaaatta tcatcgtacc gggtgctcga agcacagaat gcgcaggaag
    12781 ctatggaaat agctgtccgt gaaaaaccgg atgtcatcac gatggatatt atgatgcccg
    12841 ataaggacgg catcacgctt tgcgaagaaa tcaaagccaa tccgcaaacc aaacatatac
    12901 cggttgtcat ggtgacggta gtcggccagc gtcagcgcgc aatgactgca ggcgccgatt
    12961 attttatgaa taagtttttc accattgatg aactggtatc cgtgattgat aaactcatcg
    13021 cggataaaca aaccgcttaa aaccttctat tccacgatac gaacttttgt attttttccg
    13081 ataccgacat agtttgattt ttcctctgtc tctgtcagtt gcaccacgtc ataacgttca
    13141 attttcttaa atgccaaatt gtcgtcagat ttatcacgtt tgttcatact gtacgcacaa
    13201 cgtccgcggt cattgcaccc gaaaacccgt atcctgcctt tttcttcaaa ccgttcgctg
    13261 acaaccctca cggttgaaag ataatcacga tttccgatcg tcacatcgct cagatggata
    13321 tcgcgtttgc ccatgacgat atatgtaaac ttgagtgact gacgaatgtg acccgtcatg
    13381 ccatccagta cacgaataaa ttcaggccgt tgccacggta catcatggtg acaccagtcg
    13441 gtttcctccg taagcgcagg gcaaggtttg ttcgtaaaac aagggctgta taaaaaccaa
    13501 ccttccttta gcaggcggtc acgaaatatc agaagtcgcc ttgataaatc catcagcgcc
    13561 ggttcgatcc aaattacgaa accatcctga gtcaaggccg attcaagcca aggaagaatc
    13621 ttcgattcgg catcgggttc caactcattg aggacattgc ctgcggtgat cagatcaaat
    13681 ttattttcgg agggtacagc gtgattaaga tttaagattt gcgtacgaag cgggtagggc
    13741 agcgcgaggg ctttgtaaaa cgcggataat gtatccagtg aagaagaagc gtaatccgac
    13801 gccgtcagct cggccgtcag cgttgtttcg tttttcagcc aataggccag tcccaataac
    13861 atgctgccgg ttccggatcc tgcatccaaa acgcggaggt tttttttctg attaaaaaaa
    13921 tccgactttt ggatttctgc aagcgggatg tgcatcttcg ggaaattaat gctgccgtaa
    13981 taaagcagat atgcgtcacg aaaaactttt tgatcaagat agatccggcg aataaattcg
    14041 gaacgcttgt tttttttctt aagtccctct gagatttccg tgatcgcttc agcaaaaggc
    14101 ttgagtgtcc gtggaatcgg ctcgtcaagg tgaagttgca aaaaatcacc caggcgctga
    14161 atataatcgt caggcaaata aagcattaac caatttcccg ttcgcaaaga aatccgaaat
    14221 aatatagaac agtaaagttc ggcgatcccg ttgcgatcat tacgtcgtag cgctgatgat
    14281 atccatgccc atatatttgc gcaaaacctg cggcaccatg atcgtgcctt catccgtttg
    14341 ataattttcc ataatggcgg cgacgatacg cggcaaggcc aagcccgatc cgttgagcgt
    14401 atgcacaaac tccggttttc ccgtttggtt gtctttgtat cgtatattca tgcgccgtgc
    14461 ctgaaacgat tcaaaattgg aacaggagga aacttccagc caacgttttt gtcctgcggc
    14521 ccaaacttct aaatcatatt ttttagtttg cgtaaaaccc atatcacctc ggcacatcag
    14581 cagtgtacgg tatggtaact gcaatgcttg caataaacgt tcagcgtcca ttcgcagcga
    14641 ttcgagttcg tcgtacgagg tatcagggtg aacaaatttt acgagttcta ctttgtcaaa
    14701 ctgatgtaag cggttcagtc cgcgcacatc tttgccgtac gaaccggctt cacgccggaa
    14761 acaaggggta taggcggcat atttgattgg caattgttct gccttgagcg tcatctcgcg
    14821 atggatattg gtaaccggca cttccgcggt agggatcaga aaaaactcat cgcgtgtcac
    14881 gacatacatt tgatcttctt tatcgggtaa ttgaccggta ccggttgccg aatcggtatt
    14941 gacgacgatc ggcggctgca cttcggtata accgttatcg gccgcggagt ccagaaaaaa
    15001 attaatcaat gcccgttgaa gcttagcgcc ggcgccgcgg taaaccggga aacctgcacc
    15061 ggctatcttg acgcccgttg caaaatcaat caaattgtgt ttttcggcca attcccaatg
    15121 cggtttgagt tcgaaatccg ccgacggcat cgtaccccat gtaaagatcg tttgattgtc
    15181 ttccggtgaa gtgccttgcg gcgcggaagg gtgaggtaca ttaggaatgg tcagtaatac
    15241 atcgcgcaga tttttttcga tcactttgag ctgttcgtcc aaagctttga tttcttcgcc
    15301 gacagatttg gtttcggcga tgacggcatc ggcattttgg ccggcttttt tgagtttggc
    15361 cacttcttca gaagctttat tgcgccgggc tttcagatct tcgactttac ggataacaac
    15421 tttgcgttcg tcatcccaac gaatcacggc atccacatcg gcttcaaact ttttttcttt
    15481 ggccgcggtt ttgacacgtt ccgtatggtc acggataaat ttcaaatcaa gcatatttcc
    15541 tcaaaacatt ttgcaagtgt gaacgtactg tatattgact atacgccaac atatcaaaac
    15601 ttgccgtgta attcaaatcg aaattggatc ccgaagtatg tagagcggtt caggttctgc
    15661 agtttgctct tgcacaaagc aactttgacg aatatctttt ttctataacc gtcgaaaaaa
    15721 tcaaaagagg ttcgcatgta taaaacgatg tccgtgttga tcgttatggg tttgttgtgg
    15781 gcggcctgcc gcccttcggc tttacccccg ttgcccaaag tatatgatcc gcattccaat
    15841 gcccgcgccg aacaggcgta cgtaacacac atggcgcttg acgtacgtgt ggattttgat
    15901 aataagcaga tttcaggcag agcaaccttt gatgtggtag tgtccgataa aaatacgttg
    15961 atgttgacct tagatacgcg cggtctgacg attgattccg tcaagctgga tcagggatcc
    16021 gagatagcga attatgtatt ggatacaaat attccgaatt ccgattatgc gaacgatagt
    16081 tcggccatac aaatatcgtc cgcacaagcc acgatgggcc aagccctgcg catatccgta
    16141 gcgcctacaa ctaaaaaagc gcacgtatat tatcatacgg gtcatgaagc cgacgcttta
    16201 caatggttgg cgcccgcgca aacggaagga caaaagaagc cttttctatt tacgcaatcg
    16261 caaccgaatc tagcaaggac atggtttccc tgtcaggata gtcccggcat acgcatgacg
    16321 tacgaagcgc gcgttcaggt accgcccggt ttgatggccg tgatgagcgc cgaaagtgat
    16381 accgttttgc atgccgacgg catctatcat tttcgaatga cacacgccat accgtcgtat
    16441 ttggtggcgt tggctgtcgg tgattttggt tttcgaccct tggggcctcg tagcggcgtg
    16501 tatgcggaac atacgatgct ggcgcgtgcg gctgcagaat ttaccgatgt cgaagcgatg
    16561 atcgccgcgg ccgaaaagtt atacgggcct tatctgtggg ggcgttacga tctgatcgta
    16621 ctcccgccga gtttcccgta cggcggcatg gaaaatccga tgatgacttt tgctacgccg
    16681 acgatcgttg caggggatcg ttcgctggtg tctctggttg cgcacgaatt agcacactca
    16741 tggtccggta atcttgtcac caatgcgaca tggggtgata tctggatcaa tgagggattt
    16801 acgacgtatt ttgagcgacg tattgatgaa gccgtatttg gccgctcatt ttccgaaatg
    16861 gaagcgcatc tggggcatca ggatatgcaa gcggcattaa aagaattggg tacggaaagt
    16921 ccactaacta aactgcgatt ggatctgaat gatctggacc cggatgccgg tcattccgga
    16981 attatttatg ataaagggta tcactttttg cgttttatgg aggagcagat cggtcgtgat
    17041 agatgggatg cttttctcag gaagtatttc gccacatttg cctttcaatc catgacttcg
    17101 gaacgcctga tgaattatat gcgcacggag ctcattcgcg gggatcgtgc gttggaagaa
    17161 aagctccaac tggatcagtg gatattgggt acaggtttac cgtcggtttg tccggtgccg
    17221 caatcggatg cttttactaa agtcgaagaa gaagtaaaaa aatggaagtc gggtgtgccg
    17281 gcaaaacaac tgttgacctc atcgtggcag acccaacagt ggatgcactt tttaagattg
    17341 ttgccggaga atatgaatac agcgcaactc acaagtttgg atgaagcttt tagttttaca
    17401 cgttcgggta atgcagaaat acaatttgaa tggttccaac atgtgataac gcataagtat
    17461 acacccgctt acgcgcgtct ggaacactat cttacgcata tcgggcggcg caaattagtg
    17521 tttccattgt accgtaagat ggcatccgat ccggagatgc gcatcatggc caagcgtatt
    17581 tatgctgtag cgcgacgggg ttatcacccg gtgacgtatg gttccattga tgaaattttg
    17641 ttaaaaaagt agagaaaaat tttcacaaac gtccgcgggc tttgatgtcg agatagaggt
    17701 tgatcacggc ggaatgaaaa tgatccggca tagcatcaat tacgagcgcg ccgcgtgagc
    17761 gcgcaaaggc gatggcttct tcacgttcgc gcaaaagttc agccgttacg cctttttcaa
    17821 aagccgagtc actatgcatt accggtcgct ggagcacctc cgtcagcgct ttgtctttca
    17881 tcaaaatgaa tacgggcaga tgtttagggt acagtgaagc catactgccg agcagaattt
    17941 gagaagaata gcggtccacg atttctgtaa aaactaaaac tagagcacgg tgtctttggc
    18001 gtcgctgcag ccattggaaa gctgtcgtat aatccgattc ggttcgttcc agtcgagcgg
    18061 tatatagact ttcggctatg agggctcgtt gggatgtgtt ttttttaggt aaaagaaaag
    18121 aatgaacggc ggaaccgaat aacatcaggc cgaccgcgtc accgtgtgcg atacatactt
    18181 gggtcatgcg cgccgcggca ttgacggcgt gatcgagttt gctgagacca aacgcctcgc
    18241 tgcccatgag gcgcccggtg tctatcaaga
//
LOCUS       DAOQGF010000065        18229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1430_length_18229_cov_7.333388, whole genome shotgun sequence.
ACCESSION   DAOQGF010000065 DAOQGF010000000
VERSION     DAOQGF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_18229_cov_7.333388"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="PK937_09890"
     CDS             <1..1689
                     /locus_tag="PK937_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNH32923.1"
                     /translation="ADISAVQFKVLNIFEERAPRKNQRTVRLQSINDIHFTQGIRRDN
                     ADGNWTSLIALCSVAVLVVLIACFNFTNLMTAKAMRRAKEIAVRKISGASRWLIALQF
                     ISEALMLSVFATVLAIGMLEGLLPLLRHYLDISIPFTYTEHGHWLGIMIGVGILTGLI
                     AGFYPAWVLSSFEPARTLKGVAKLPAANKLRKGLVLSQFTVSIILASCTVIVWQQINH
                     MKEFNLGFQKEQIIVISAPGSLREKPETVRQQLTNDGSIVSVTFANSVPGNAFSHGPY
                     EILGGTENGRISINTIYADAEYLKTLNIALSEGRYFYPEHAAENTNYYVVNEAFMKAY
                     GKSDFQTMRLRSLDGNHDKPGLIVGIVKDFHYASLKSAIAPLVLRYAPAESWRMIIRV
                     APQRTKQAITKLSSVWQTMAPEIPMTYRFLDEVFDKQYKQEDRLHHLMIGFSALAILI
                     AGLGLYSMVAYYNEQRTKEIGIRKVLGATITDVLYLMSRDYVGMVILANIISFPLIYF
                     MMNHWLDDFAYRVTVRWEIMAITGILTLGLTLIMVGIQSWRTASANPIHALKYE"
     gene            1723..4077
                     /locus_tag="PK937_09895"
     CDS             1723..4077
                     /locus_tag="PK937_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32924.1"
                     /translation="MLKSYLTMALRTLSKSKAYSFINIAGLAIGLTCTTLILLWVQDE
                     LSYNRFHEKLPRLYRVMESQHYANGEIFTTSATPAPLLYHLKERFPEFEGISRMNFWN
                     NILIEYGEKRMYENDGLGVDPDFLTMFSFPLIKGDPLTALNQPDAVVLSETLAKKYFG
                     NEDPIGKTIKMGGRDLFKITAIAADPPLQSSIKFNLLWSLSYLKETQPDKSNWDNNWL
                     RLFAVLQPNQKGTDVSEKIKNVIIDRNPNMAVRIELFLQPLSDMYLYSDFNKIPPRIN
                     YVYILSGIAFLILLIACINFTNLATARSIKRAKEVGLRKVVGADRFQLIRQFLIESVT
                     ITLVAMMLSLILIELLIPLFNDLSGKQLSLRWDDIQTFSLMCAMVLFTGLVSGLYPAM
                     VLSRYQPALVLKGSFSKSIQGMRLRKILVSLQFTLSIALVICTIIAYQQLQYMTNKNL
                     GYDKEHLVYIRLLDDTAKQYPVLKEKLQQKPGIVSVSAADQTMTGFGSNTWGLKWRGQ
                     QEGQRILTTFLRVDYDFFETHGIQVSEGRFFSKNFAADSGAFIVNEAAIRQMKLENPL
                     QETITLWNTQGPIVGVVKDFHFQSVHQEITPLIFILKPERVNVMTVRIGPSETTKTLA
                     SIEETWQQVMPQYPFVYQFVDEELEKLYKAEQRLTGIYTSFTVLAIIISCLGLFGLAS
                     FTTEQRTKEIGIRKVLGASIPKIIIMLSTEFLKLVLLSNIIAWPVAYFLMSRWLQDFA
                     FRVDIGIMTFILAGTTALCIALITVSYQAIKASSANPVDALKYE"
     gene            4495..6891
                     /locus_tag="PK937_09900"
     CDS             4495..6891
                     /locus_tag="PK937_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32925.1"
                     /translation="MLYSYIRTAFRNIYRHKSVSAINLIGLTTGMTACLLIALYVHDE
                     LSYESQHTKADRIYRLNCTYYLPKNAGTEAYATTGPGVATAIAKDYPEIASVVRIRKI
                     DDFTLQKRNAIERFYETVMFADSTLFDVFSLPLIYGESKNALSSPLSMVISKSMALKY
                     FGKENAIGESFIVNDTLSCRIAGIIDDPRKPSHLEFNMIVSMVSMPVMHINLESWWNF
                     SYHTFVLLHPGTDVASFEEKMKNISRRYILSQEQNSGYSQEYNITNLRHIHLDSHLRS
                     ELSENGRRAYVYTFAAIGGLILLIASINFINLTTARVMTRAREVGVRKVVGAHRRHLV
                     YQFFIESALMIIITSGLCIVTAELTLPWLNTVTGKKLFIDIFNDPIISIILFTLTSVV
                     CIGAGLYPALILSSFAPLSIIKSFKSSSRGILLRKVLVITQFVISIGLIAGIVLVHRQ
                     IAFMRNAQLGFSKEQTMILALREPVGNPQLQMYQNEFGNIPGVQLVTASSRVPGKELG
                     NNVVRLGWDDKAEWSDMRFLSADYEFLAAYDLRIMTGRWFERTRPSDENEAFVINESA
                     VTRLGFVSAEDALGKPLRWQRKKGRVIGVLKDFHFMPMQHHIEPFIVVMANSHDANYL
                     SLKLRSDNMPAMVEQIHSTYTRLMPGQSFEYSFLDESFDRLYHAEDRFNEIIFVFTGL
                     AILVACLGLFGLVSFTTEQRTKEIGVRKILGATTAHIIALIAKDFLWLIMIAVILATP
                     LAYYAFSTWLHNFPYRTDIVWWAFPVAGCIAMVLAAITMSVQSVKASSANPVDALKYE
                     "
     gene            6948..9317
                     /locus_tag="PK937_09905"
     CDS             6948..9317
                     /locus_tag="PK937_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32926.1"
                     /translation="MLFNFLKVSLRNMWRNRAYAAINIIGLTVGLTACFIIGVYVIHE
                     TYYDRFHEKSERIYRIHSDTRGFGLSNASSPALFSELYKYNAIASATRVFRHWFDPLI
                     SRDKENGFIESQFFFADSSVFDVFSLTLLAGEGGEALGRPMTVLLTASTARKYFGKQS
                     PVGSILKYNAEHEFTVAGVIDDFPSTSHVRPDFIASMGSTEKIFWPTFLKSSGNLVKT
                     YIVVRPEASVENLAADLTSIYTTRYGKDNRVTLSLMPLTDIHLKSTVEKDFSINNDIR
                     YVQLLGIIGLIIILISLINYTNLTAARSLTRSKEVGIRQTLGGFRSMIVIQFLCESFL
                     FVLLGLVFSMVLSEMLFPAINRMTSIHLSFSLLESTHWIMIAAAVVLLAITGGVYPAW
                     IVARFKIVHTLKGRVEHVRGSWLRKGLVISQFVSGVILIVATSVIWHQMQFIREARLG
                     FTKEEVAIVRLMDPSVASNLPALKQRWLNIPGVQSVALANAMPGKTHAGDYISHRGTD
                     EDLAVAVNWIDEAFVPTMDLSIIAGRNISPAHPTDNDGSVLINEATARELGYPDPEKA
                     IGTLIALQGTDGGKQRTVVGVVNDFHYESFRHKITPLILVPQFGRCAYLMVRCNTQAL
                     DQTLDHIKTVWSEMATEQPFAFTFLDDSFQQLYAQDRRWSLVVGAGASAALIIACLGL
                     FGLVIFNVQRRVKEIGVRKILGATIGNIIRLLTKEYLLLIGAANMIAWPIAYYWMQEW
                     LTQFVYRPSFPWLAFPLALVATMSMAMLTIGFQAFKAAVTNPIHSLKYE"
     gene            9447..11858
                     /locus_tag="PK937_09910"
     CDS             9447..11858
                     /locus_tag="PK937_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32927.1"
                     /translation="MISNYIKIALRNLLKHKLYSVINILGLAVGIAACLVIMIFIKDE
                     LSYDRHHPQADRIHRITRSWNNANGETSLHLARVAPTVAPLMRNDFPTVPVTQILQDF
                     QTRLTYENNVFIENRLYFADEFAFTVFGFKTIKGNRQDALKNPNELWITESMAKKYFG
                     VVDPVGKTIRYENEIDLQVVGVLADPPPQTHFHYDFLVSFSTFKTLVPPDALTDWGSN
                     GYITYAMLPTDMTSVELEEKFISFVDRYIPPKNSAFPKPSMGTRLHVQKITDIHLYSH
                     LDSEIEPNGNILYIWIFSLVAVFVLMIACINFMNLATAKASERATEVGVRKAIGAHRG
                     DLIRQFLSESLVITAIAFLLAIGLVELSLPVFSAFIEKTLELTLVENYSMLIFVLAVA
                     FLTGILAGSYPAIYLSSFEAAKVLKEKSRFGSARSRFRSALVVAQFAISIILIIGIGV
                     IQSQLDYCRNTDMGFDKDRIVSLFIEQETRGKIPQVKTQLLQSPGIEAVAASSRIPSG
                     RLLDSYKMSVESEGTMKEIDFRIAAVRVDEDFFPTYGIPMTSGRNFSKDFSTDDTAAV
                     VFNESSVAAIGWKSSNDALGKTVIAGGRTMKVIGIVKDFHFESMHEKISPMVFFPNTG
                     KYARLSIRLRGARIPETIEYIKTRWAEYHHGIPFEFQFVDENFDQLYRAEERLGSILR
                     TFAALATFIACLGLVGLAAFMAERRTKEIGIRKVMGATTFNIVRLLSSDFAKLVLLAN
                     VIAWPAAYYVMKEWLATFVYHTDISILIFIVSGAVTLSIAILTVAFQAFSAANANPIR
                     SLKYE"
     gene            11889..14297
                     /locus_tag="PK937_09915"
     CDS             11889..14297
                     /locus_tag="PK937_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32928.1"
                     /translation="MLTNYIKIALRNMLKAKGYSAINILGLAVGLACCLLIVLFITDE
                     LSYDTHYANGDRLYRVYLNARVNNKDLVTVTTNSPLAAAMMKEIPEVETAGRLYSPGN
                     FTIRYGEKIFNEERIYFADPTMLDIFSVTVREGDVKNFDQPNMIILSESMAKKYFSDE
                     NPIDKVLRLDGKTDYKVIAVYNDVPGNAHLHFDFLASLTNREEGKNTQWLSNNFSTYT
                     RLKPGASTTGVESKMREMVMRYGGPELQRYANITFEQFFSQGGAYAYHLQPIRDIHLY
                     SNLENEQEPGGDITYVYIFSGIAIFILLVACVNFMNLATARSAGRAKEVGIRKVLGSE
                     RGQLIRLFLIESLVITTLAMIIGVLLAEMALPFFNELSGKSLHIGIATNPMLGIGLVL
                     AVLLVGVLAGTYPAFYLTAYQPIAVLKGTLASGVKNSGLRNVLVVFQFCVSIILIAGT
                     IIVYQQLQYIQNRNLGFQKEQILVIHNVWQMNKQGDAFKQSVLRTPGVASASLTSTLP
                     GHPTGNSAFQIEGDASNEPYLLWQVRTDFDFLKTMGIDMAQGRDFSIEHPSDSTAIII
                     NEAAVRLMNMPQPLGKRLTRFSSAPYEIVGVTQDFNFQSLRNPVRPISIFMNPPGFPA
                     PSFLAVRVKPDHLQETIQSIGQVWNQFQPGEPYVYTFLDEDFGKLYRTEERAGTIVTT
                     FSSLAIFIACLGLLGLAAFATERRTKEIGIRKVLGASEGHIIGMICKEFVILVTVALV
                     ISVPIAYYVMNRWLQDFAYRIDIGADVFILSGVIALTVALLTVSYTAIRAALSNPVTA
                     LKYE"
     gene            14290..14784
                     /locus_tag="PK937_09920"
     CDS             14290..14784
                     /locus_tag="PK937_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH32929.1"
                     /translation="MNNVSSIEIRAIENRDNPALASIIRTVMPEFGADGPGFAIHDAE
                     VNDMFAAYTRPRSAYFVVVADGVVSGGGGIAPLAGGDANVCELKKMYFLPGLRGRGVG
                     HKIITFCLQSAMAFGYKQCYLETLTGMDAAQHLYKQHGFRSIPQAMGQTGHYSCDRFY
                     IKDI"
     gene            14819..15097
                     /locus_tag="PK937_09925"
     CDS             14819..15097
                     /locus_tag="PK937_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32930.1"
                     /translation="MAEVVIQLPELNSKDRIEVECRINGKKQVLHYRVEIFAWEDCNE
                     PVADRALCLKRLVENYDHNWQLQQIGAYTDKTVQVMFREKHTPGTLIH"
     gene            15108..15770
                     /locus_tag="PK937_09930"
     CDS             15108..15770
                     /locus_tag="PK937_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH32931.1"
                     /translation="MITLKNIEKYYENKSIKNYVLRDINLTIQPGEFVSIMGPSGSGK
                     STLLHILGMLDEPTKGEYLFLDQEVHRISERQKTELHKNHIGFVFQSYHLIDELTVYE
                     NIETPLLYKKTSGSERKALVADILDRFNIVGKKDLFPSQLSGGQQQLVGIARAVITAP
                     KVILADEPTGNLNSAQGEEIMQLFKKLNQEGTTIIQVTHSEKNAAYGNRIINLLDGFI
                     QK"
     gene            15888..>16311
                     /locus_tag="PK937_09935"
     CDS             15888..>16311
                     /locus_tag="PK937_09935"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH32932.1"
                     /translation="MIKLLQPTIRPDDYVLDAGCGDGNATRMLLKIHPQVRIMGIDQS
                     PSMANPIEHSGRTEIRIASLDAIAEENHTFDAVMCIGVTEYIDDIPRVLLEIKRATKP
                     GAPMVVTFAPPVFWNYLRFGYGKTLRLISTTEATHFLQK"
     assembly_gap    16312..16411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16412..16854)
                     /locus_tag="PK937_09940"
     CDS             complement(<16412..16854)
                     /locus_tag="PK937_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32933.1"
                     /translation="MRRRIAGNIGFLSILLTMVSCTSYIPFSYKGYDNKTQYSTNIGS
                     TMIYRESGYKKATAPDTGRTTDPLQINTIHHGSRYELIYAGINHNVIQILYREYVVRI
                     NQGYYEPVFSQIFQYDINENKSITFQNYKIQVFEANSELIRFQVLS"
     gene            17060..17410
                     /locus_tag="PK937_09945"
     CDS             17060..17410
                     /locus_tag="PK937_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNH32934.1"
                     /translation="MQSYMMIFRGGDDAMVKMSPDEIQQHMGQWFAWVEKLTKEGRYV
                     SGEPLVMEGKVVRAKGVITDGPFAEGKELVGGFFIVKAKSIEEACDMAKVCPSFQFDG
                     ATVEIRQVQEMEHA"
     gene            17420..>18229
                     /locus_tag="PK937_09950"
     CDS             17420..>18229
                     /locus_tag="PK937_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH32935.1"
                     /translation="MSVGENHPSIESLVDHLFRNSAGKMVSVLTRLFGSHNITLAEDV
                     VQDTLLKAMQIWPRAGIPQNPEGWLMQVAKNRALDLIRRRSREIELPEDTDPLLSSAW
                     TRATRMEYLFLPHEIRDDQLRLMFTCCHPMLPQESRIALTLKTLCGLGTKEIARAFLT
                     SEETIQKRLYRAKQLLREHRIRFEIPDDGISERFNSVLHVIYLLFNEGYNTTRTDAVI
                     RQDLMDESIRLGEMLLQNNRTNTPATQALLALMYLHAARTDARTDATGHILL"
BASE COUNT         4804 a         4350 c         4021 g         4954 t
ORIGIN      
        1 gccgacatta gcgcggtaca gtttaaggta ttgaatattt tcgaagaacg tgcaccgcga
       61 aaaaatcagc gcacagtccg acttcagtct atcaacgata ttcatttcac gcaaggtatc
      121 cgccgtgata acgccgacgg aaattggact tcactgatcg cgctttgcag cgtcgccgta
      181 ttggtcgtgc tcatcgcatg tttcaatttt accaatctga tgacggccaa agccatgcga
      241 cgcgccaaag aaatcgccgt acgaaaaatt tccggcgcaa gccgatggct catagcgctg
      301 cagtttatca gcgaagcgct aatgctgtcg gtttttgcta ccgtcttggc tatcgggatg
      361 ctggaaggct tactcccgct tttacgtcat tatctcgaca tatctattcc ttttacctat
      421 accgaacacg gtcactggtt aggcattatg atcggcgtcg gaatattgac cgggctcatc
      481 gccggttttt accccgcatg ggttttatca tcctttgaac ccgcgcgcac cctcaaaggc
      541 gtggccaaac ttccggcggc caacaaactc cgcaaaggat tggttttatc gcaattcacc
      601 gtttcgatca ttctggcttc gtgcacggtg attgtttggc aacaaatcaa ccacatgaaa
      661 gaatttaacc tcggctttca aaaagaacag attatcgtga tttcggcccc gggttcattg
      721 cgtgaaaaac ctgaaaccgt gcgtcaacag ctcaccaacg acggctcgat cgtatccgtg
      781 acgtttgcca actccgtacc cggcaatgct ttttcgcatg gcccgtatga aatactgggc
      841 ggaacggaaa acgggcgcat ttctatcaat accatatatg cggatgcgga atatttgaag
      901 acgcttaaca tagcgctatc cgaagggcgc tatttttatc cggaacatgc agcggagaac
      961 acgaattatt atgtggtcaa cgaagctttt atgaaagctt acggaaaatc ggattttcaa
     1021 acgatgcgct tgcgttcatt ggacggaaat catgataaac cgggattaat cgtcgggatt
     1081 gtcaaagact tccattacgc atcgctcaaa agcgctatcg caccattggt cctgcgctac
     1141 gctccggcag aatcctggcg catgattatt cgcgtcgcac cacaacgaac aaaacaggct
     1201 ataacaaaac tttcgtccgt gtggcaaact atggcgccag aaattccgat gacgtaccgc
     1261 tttcttgatg aggtgtttga caaacaatat aaacaggaag atcgcctgca tcacctgatg
     1321 atcggctttt cggcactggc cattcttatt gccgggcttg gcctctatag tatggtggcg
     1381 tattacaatg agcagcgtac caaagaaatc ggaattcgca aagttttggg cgccacgatc
     1441 acggatgtac tgtatctgat gtcgcgcgat tatgtcggaa tggtcatcct ggcaaacatc
     1501 atttcattcc ctctgattta ttttatgatg aatcactggc tggacgattt tgcctatcgc
     1561 gttaccgttc gatgggaaat catggctatt acgggaatat tgacattagg attgacactc
     1621 atcatggtgg gaatacaatc ctggcgcacg gcttccgcca atccgatcca cgcactcaaa
     1681 tacgaataaa cgaaccttat cgaataaaag caggaacatg tcatgctcaa aagttatctc
     1741 actatggcct tgcgtacgtt atccaaaagc aaagcttatt ctttcatcaa tatcgccggt
     1801 cttgccatcg gactcacgtg tacgacgctg atactcctgt gggtgcaaga cgaattgtct
     1861 tataaccgtt ttcatgaaaa actaccgcgc ctctaccgcg tgatggaaag tcagcactat
     1921 gccaatggtg aaatttttac gacatcggct acgccggctc cgctgctgta ccatcttaaa
     1981 gagcgctttc ctgaattcga aggtatttcc cgaatgaatt tttggaataa tatacttatc
     2041 gaatacggcg aaaaacgcat gtatgaaaac gacggtctcg gtgtggaccc cgattttctt
     2101 accatgtttt cttttccatt aatcaaaggc gatccgttaa cagcgctcaa tcaacccgac
     2161 gccgtagtac tcagcgaaac cctggctaaa aaatatttcg gaaatgaaga ccctataggt
     2221 aaaaccatca aaatgggtgg ccgcgactta tttaaaatca cggctatagc tgccgatcct
     2281 ccgttgcaat ccagcattaa attcaatctt ttgtggtcct tgtcgtatct gaaagaaacg
     2341 caaccggata aaagcaattg ggataataac tggctgagac tgttcgccgt tttacagccc
     2401 aatcaaaaag gcacggatgt aagtgaaaaa ataaaaaatg tgattattga tcgtaatcct
     2461 aatatggcgg tccgaattga attattctta caacctctaa gcgacatgta cctgtactcg
     2521 gatttcaata aaataccgcc acgcatcaac tacgtttaca tcttatcagg tatcgcattt
     2581 ttaatacttt tgatcgcatg tattaatttt accaatctcg ctacggctcg ctcgatcaaa
     2641 cgcgccaaag aagtcggatt acgcaaagtc gtaggggccg atcgttttca actcattcgc
     2701 caatttctga tcgaatccgt aacgatcacg ttagtcgcca tgatgttatc gctcattctg
     2761 atcgagctgc tgattcctct ttttaacgat ctctccggaa aacaattatc cttacgttgg
     2821 gacgacatac agacgtttag tttgatgtgt gcgatggttc tattcacggg attggtttca
     2881 ggattgtatc cggctatggt actcagccgt taccaacctg ctctcgtact caaaggttca
     2941 ttcagcaaga gcatacaagg catgcgcctt cgaaaaatcc tcgtatcgct tcagtttaca
     3001 ttatccatcg cgctcgtcat ctgcaccatc atcgcatatc agcagcttca gtatatgacg
     3061 aataaaaacc tcggctacga caaggaacat ctagtttaca ttcgtttact ggatgatacg
     3121 gccaaacaat accccgtact caaagaaaaa cttcaacaga aaccgggtat cgtatcggtt
     3181 tctgccgccg atcagacaat gaccgggttt ggaagtaaca catggggatt aaaatggcgc
     3241 ggtcaacaag aaggccaacg catactgact acttttctgc gtgtggacta tgattttttt
     3301 gaaacgcacg gcattcaagt ttctgaagga cgatttttct cgaaaaattt tgccgccgac
     3361 agcggcgcct ttattgtcaa cgaagcggct atccggcaaa tgaagttgga gaatccgctt
     3421 caggaaacga taacgctatg gaacacgcag ggccctatcg tcggtgttgt caaagatttt
     3481 cattttcaat ccgtacatca ggaaatcacg cctttgattt ttatactcaa accggaacgc
     3541 gtgaatgtaa tgaccgttcg catcgggcca tcagaaacca ctaaaacttt agcgtctatt
     3601 gaagaaacgt ggcaacaagt aatgcctcaa tatccttttg tatatcaatt tgtagacgaa
     3661 gaacttgaaa agttatataa agccgagcaa cgcctgaccg gaatatatac gtctttcacc
     3721 gtactggcga tcatcatatc ctgtctcggg cttttcggat tggcctcttt tacgacagaa
     3781 cagcgcacca aagaaatcgg cattcgtaaa gtgctcggcg cctcgattcc gaaaatcatc
     3841 atcatgcttt cgacggaatt cctaaagctc gttctattgt ccaatattat cgcctggccc
     3901 gtagcgtatt tcctgatgag tcgctggctt caggattttg cgtttcgcgt ggacatcggc
     3961 attatgacat ttattctggc cggcaccacg gcgctttgta ttgcattgat taccgtcagc
     4021 tatcaagcga tcaaagcttc atccgctaat cctgtagatg cgctgaaata tgaataaggg
     4081 atttcatact aatccaagaa aggaaatggc atgtaccatc gcttaacacg caatatttta
     4141 ttgatcgttt ttattattgc agcgatagca tttttaagta tgcgacacga agaccttttt
     4201 ttttgcaatg attcgtattt cccgggctca tcaaattcat ttcacaacgt tcgtcatccc
     4261 cattatctcc ggatgcatac aaatatatca aaggaatcgg atgagaattt tttcacagat
     4321 gatctgtgta attccgaaaa ataccgtcga ggcttttatc atagaaaaat atccaaaaca
     4381 catgaacgat acgaaatgcg caggcattgg gatcggttaa atacccatca agaatggata
     4441 cggcgctaaa tctaatatga gttcataata ttaaaccatc ctcaaaggaa gtttatgtta
     4501 tacagttata tccgtacggc tttcagaaat atctatcgac acaaaagcgt ctctgcgatc
     4561 aacctcatcg ggctgacgac cggcatgaca gcgtgccttc tgatcgcatt atatgtacat
     4621 gatgaactga gctacgaatc acaacatacc aaagcggatc gcatctatcg tttgaactgt
     4681 acgtactacc ttcctaaaaa tgcaggcacc gaagcatatg ccacgacagg acccggcgta
     4741 gcgacagcga ttgccaaaga ttatccggaa attgcatcgg tagttcgcat ccgaaaaatt
     4801 gatgatttta cgcttcaaaa gcgtaacgcc atcgagcggt tttacgaaac cgtgatgttc
     4861 gccgattcga ctcttttcga tgtttttagt cttccactga tttacggcga atctaaaaac
     4921 gccctctcat cgccgctttc gatggtcatc agcaaatcca tggcgctaaa atatttcgga
     4981 aaagaaaacg ccatcggtga atccttcata gtcaatgata cgctttcgtg ccgcatcgcg
     5041 ggcatcatag atgacccgcg caaaccatcc catcttgagt ttaacatgat tgtttccatg
     5101 gtaagtatgc ccgtaatgca cattaacttg gagagttggt ggaattttag ctatcacacg
     5161 tttgttttat tgcaccccgg tacggacgtc gcttcatttg aagaaaaaat gaagaatatt
     5221 tcacggcgtt atattttatc tcaggaacaa aattccggtt attcgcaaga atacaatatt
     5281 accaaccttc gccatattca tttggattcg catttacgat ccgaattaag cgaaaacggc
     5341 aggcgggctt atgtgtatac tttcgccgcc atcggcggac tgatcctact gattgccagc
     5401 atcaacttta tcaatcttac gacagcccgt gtcatgacac gggcgcgcga agtcggtgtc
     5461 cgaaaagtcg tgggcgctca ccgacggcat ctggtgtatc aatttttcat cgaatccgca
     5521 ttaatgataa ttattacttc agggctctgc attgtaacag ccgagcttac gttgccttgg
     5581 cttaacacgg tgaccggcaa aaaacttttc atcgatattt ttaatgaccc gattataagt
     5641 atcatcttgt ttacgcttac ctcggtcgta tgcatcggcg ccggtcttta tcctgcactg
     5701 atactctcat cctttgcgcc actgtccatc atcaagagtt tcaaatcgtc atcccgtggc
     5761 attttgcttc gcaaggttct cgtaattaca cagtttgtca tatcgatcgg gttgatcgcc
     5821 ggtattgttt tggtgcaccg gcagattgca tttatgcgaa acgcccaact tggattttct
     5881 aaagaacaaa ccatgatcct cgctttacgc gaacctgtcg gcaatccgca gttacaaatg
     5941 tatcaaaacg aattcggcaa tatccccggc gttcagttgg tgacggcatc ctcacgcgtt
     6001 cccggaaaag aactcggcaa taacgtcgtt cgtctgggtt gggatgacaa agccgaatgg
     6061 agtgatatgc gttttttatc ggccgattat gaatttctcg ccgcctatga tctgcggatt
     6121 atgaccggtc gctggttcga acgtacacgc cccagcgatg aaaacgaagc gtttgtaatc
     6181 aatgagtccg ccgttacgcg gcttgggttt gtatcagccg aagatgctct cggtaaaccg
     6241 ctgcgttggc aaagaaaaaa agggcgtgtt atcggagttc ttaaggattt ccattttatg
     6301 cctatgcagc accacatcga accctttata gtcgttatgg ccaattcgca tgacgccaac
     6361 tatttatcgc tcaaattacg gtcggacaat atgccggcaa tggtagaaca gattcattcg
     6421 acctacacgc gcttgatgcc ggggcaatcg tttgagtact cttttctgga tgaaagtttt
     6481 gaccgattat accatgcaga agatcgtttt aacgagatca tcttcgtttt cacgggtctg
     6541 gctattctcg ttgcatgcct tgggcttttc ggattggtat cgttcacgac ggaacagcgt
     6601 actaaagaaa tcggcgtgcg aaaaatactc ggcgccacga cagcacatat catcgcattg
     6661 attgccaaag attttctttg gcttatcatg atagccgtaa ttctcgcaac acctttagcg
     6721 tattatgctt tcagcacatg gcttcataat ttcccctatc gaacagacat cgtatggtgg
     6781 gcgtttcctg tagcaggatg catcgcgatg gtactcgcgg caataacaat gtccgtacaa
     6841 tccgtcaaag cttcatccgc caatcccgtg gatgcactga agtatgagta aaaagttttg
     6901 aataaatacg ttaatcgctg catcaaaaaa acaccgaaag gccgccaatg ctgtttaatt
     6961 tcctaaaagt cagcctgcgc aatatgtggc gcaaccgtgc ttacgccgcg atcaatatta
     7021 tcggtttgac cgtaggtctg acggcatgtt tcatcattgg cgtgtatgtg attcatgaaa
     7081 catactacga ccggtttcat gaaaaatccg aacgcatcta tcgtattcat tcggacacgc
     7141 ggggttttgg tttatcgaac gcctcttcgc cggcattgtt ttcagagtta tataaataca
     7201 atgcgatagc aagcgcaaca cgggtctttc gtcattggtt cgatccgttg atctcacgcg
     7261 ataaagagaa cggttttatc gaaagtcaat ttttctttgc cgattcgtcc gtatttgatg
     7321 tgtttagttt aactcttctc gccggtgaag ggggagaagc gcttgggcga ccgatgaccg
     7381 tattattaac cgcttcgact gcgcgcaaat atttcgggaa acagtctccg gtaggatcaa
     7441 tattaaaata taatgccgaa catgaattca ccgtcgccgg cgttattgac gattttccgt
     7501 ccacttcgca tgtgcggcct gattttatcg cttcgatggg ttcgacggaa aaaatattct
     7561 ggccgacttt tttaaaatca agcggcaacc ttgtaaaaac ctatatcgtc gtacgcccgg
     7621 aggcatcggt cgaaaacctc gcggccgatc tgacaagtat ttatacgacg cgctacggta
     7681 aagataatcg tgtcaccctc agcctgatgc cgctcaccga cattcatctc aaatccaccg
     7741 tcgaaaaaga ttttagtatc aacaatgata tacgctatgt acagttatta ggtatcatcg
     7801 gattgattat cattttgatt tcgctgatca actataccaa tcttaccgcg gcgcgaagcc
     7861 tcacgcgcag taaagaggtg ggtattcgcc aaacgctcgg cggttttcgc tcgatgatcg
     7921 tcatccagtt tttatgcgaa tcatttttat tcgttttgtt aggactcgtt ttttcgatgg
     7981 tcctttcgga aatgttgttt cctgcgatta atcgtatgac ctccattcat ctcagttttt
     8041 cgttattgga aagcacacac tggattatga tcgccgcggc cgtcgtcctg ctggccataa
     8101 cgggcggggt ctatccggcc tggatcgtcg cgcgatttaa aatagtccat actctgaaag
     8161 gacgggtcga acacgtacgc ggcagttggc tgcgcaaagg tctcgtcatt tcgcaatttg
     8221 tttccggtgt aatcctgatc gtagcgacga gtgtgatctg gcatcagatg cagtttatcc
     8281 gtgaagcgcg attaggtttt actaaagaag aagtagcgat cgtacgtctg atggatcctt
     8341 cggtcgcatc caatctcccc gcgctcaaac agcgttggct caatattccg ggtgtccaat
     8401 ccgtagcctt agccaatgct atgcccggta aaacacatgc cggtgattac atttcacacc
     8461 gcggcacgga tgaagacctg gccgtagccg tcaactggat agatgaagct tttgtgccga
     8521 ccatggatct ttcaatcatt gccggacgca atatttcacc cgctcatccg accgacaatg
     8581 acggatccgt tttgatcaat gaagccacgg cgcgtgaact gggatacccc gatccggaaa
     8641 aagcgatcgg cacgctgatc gcccttcaag gaacggacgg cggtaaacaa cgaaccgtcg
     8701 tcggtgtggt aaatgatttt cattacgaaa gttttagaca caaaattacg ccgttgattc
     8761 ttgttcctca gttcggacga tgcgcctacc tgatggttcg ttgcaatacg caagcattgg
     8821 atcagacact ggatcatatc aaaaccgtct ggtcggagat ggccacggag caaccgtttg
     8881 ccttcacgtt tttagatgac tcatttcagc agttgtatgc gcaggatcgt cggtggtctc
     8941 tggtcgtcgg tgccggggct tcggccgctc tgatcatcgc atgcctcggc cttttcggat
     9001 tagttatttt caacgtccaa cgccgcgtca aagaaatcgg agtacgcaaa atactcggcg
     9061 ctacgatcgg taacattatc cggttactta ctaaagaata tcttttgctg atcggtgcag
     9121 ccaatatgat cgcttggccg atagcctatt attggatgca ggaatggctc actcaatttg
     9181 tttaccgccc ttcgtttcca tggcttgcat ttcctttggc gctggttgca acgatgagca
     9241 tggccatgtt gacgattggt tttcaggcat tcaaagcggc tgtgaccaat ccgatacatt
     9301 cactcaaata tgaataatgc gtgtagggtc aaaacgcatc atttttatgg caaccttata
     9361 tagagttcag gcgtatagtt aaaagtataa tgcagcattt ggagtaacaa aatgtaccga
     9421 tatatccact ttgaaaggca cggcgcatga tatccaatta tatcaaaatc gctttgcgca
     9481 atttacttaa gcacaaattg tattcggtaa tcaatattct cggtcttgct gtcggcatcg
     9541 ccgcatgtct tgtgattatg attttcatca aagacgaatt gagttacgat cgacaccacc
     9601 ctcaggcgga tcgtatacat cgcattaccc ggtcatggaa taacgctaat ggtgaaacca
     9661 gtctgcatct ggcgcgtgtc gctccgacag tagcgccgct gatgcgcaac gactttccta
     9721 ccgtccctgt aacccaaatc cttcaggact ttcaaacacg tctcacgtat gaaaacaacg
     9781 tgtttattga aaatcgtttg tactttgcgg atgaattcgc attcactgtt tttggcttca
     9841 aaaccatcaa aggcaaccgt caggatgctt tgaaaaatcc caatgaactg tggatcactg
     9901 aaagcatggc taaaaaatat tttggagtcg tcgatccggt cggcaaaacc atccgttatg
     9961 aaaatgaaat tgatcttcaa gttgtcggag tacttgctga tccgccgccg caaacgcact
    10021 ttcattatga ctttcttgtt tcattcagta cttttaaaac cttagttccc ccggacgcac
    10081 tcaccgattg gggttccaat ggctatatca cttacgccat gctaccgacc gatatgacct
    10141 ctgtagaact cgaagaaaaa tttatctctt ttgttgaccg atacattccg cccaaaaatt
    10201 cggcattccc gaagccaagt atgggtacgc gattgcatgt ccaaaaaata acagatattc
    10261 acctatactc acaccttgat tctgagatcg aacccaatgg aaatattttg tacatttgga
    10321 tattttctct ggtagcagtg tttgtcttga tgatcgcatg tatcaatttt atgaatctgg
    10381 caacagccaa agcctcggaa cgcgccacgg aagtcggggt gcgcaaagcg atcggcgcac
    10441 atcgcggcga ccttatccgt caatttctga gcgaatcgct cgtaatcacc gcaatcgcat
    10501 ttttgctggc aattggtttg gtcgagctat cgcttcctgt attttctgca tttatcgaaa
    10561 aaacactcga attgactttg gttgaaaatt actccatgtt gatatttgta ctcgccgtag
    10621 cattcctcac gggcatacta gccggaagtt atccggcgat atatctttcg tcgtttgaag
    10681 ccgccaaagt actcaaagaa aaaagccggt ttggttccgc acgctcaaga ttccgctcgg
    10741 cactcgtggt cgcccaattt gctatttcta ttatactcat cataggcatc ggcgttattc
    10801 agagtcagct cgactattgc cgcaataccg atatgggatt cgataaagat cgtatcgttt
    10861 ctctttttat agagcaagaa acacgcggaa aaattcctca ggtaaaaacg caactcctgc
    10921 aatctcccgg tattgaagct gttgcggctt ctagccgcat cccgtccggt cgtttattgg
    10981 atagttataa aatgtccgtg gaatccgagg ggacaatgaa agaaattgat tttcggatag
    11041 cggccgttcg tgtagatgag gatttctttc cgacctatgg tataccaatg acgtctggga
    11101 gaaatttttc aaaggacttt tccacggacg atacagccgc tgtagtattc aatgaaagtt
    11161 ctgtcgctgc cattgggtgg aagtccagca atgatgccct ggggaaaacg gtcatagccg
    11221 gcgggagaac gatgaaagta atcggcatcg tcaaagattt tcattttgaa tccatgcatg
    11281 aaaaaatttc accgatggtg ttttttccaa acactggaaa gtatgcccgc ctttcaatcc
    11341 gtctgcgagg cgcacggatt ccggaaacta tagaatacat aaaaacccgg tgggccgaat
    11401 atcatcatgg tattcctttt gagtttcagt ttgttgatga aaatttcgat caactttatc
    11461 gcgccgaaga gcgtttgggt tccatactcc gtacgtttgc ggcactcgct acgtttatag
    11521 catgtctggg tcttgtaggc ctagccgctt ttatggccga gcgtcgcacc aaagaaatcg
    11581 gcattcgcaa agtgatgggc gcaaccacat ttaatatcgt gcgcctatta tcatccgatt
    11641 ttgcaaaatt ggtattgtta gccaatgtga tcgcctggcc tgcggcgtat tatgttatga
    11701 aagaatggct cgctacattc gtgtatcata cggacatttc tattttgatt tttatcgttt
    11761 ccggagccgt tacgctgagt attgcgatat tgacggttgc gttccaggcc ttttcagccg
    11821 ccaacgccaa cccgatacgg tccctaaaat acgaataaca tgatttatat attcaggagt
    11881 acacacgcat gttgaccaat tatatcaaaa tcgctttacg caatatgctc aaagccaaag
    11941 gctattcggc tatcaatatt ttaggattgg ccgtcggtct ggcatgttgt ttattgatcg
    12001 ttctttttat caccgacgaa ctgagttacg atacccatta tgccaacggc gatcgtttgt
    12061 accgcgttta tctgaacgct cgcgtcaaca acaaagacct tgtcacggta accacgaata
    12121 gccctctggc ggccgctatg atgaaggaaa tccctgaagt cgaaacggcc ggtcgtctgt
    12181 acagtccggg aaattttacg attcgttacg gtgaaaaaat ctttaatgaa gagcgtatat
    12241 attttgccga tccgacgatg ctggatattt ttagtgtgac cgtccgcgaa ggcgacgtca
    12301 aaaattttga ccagcccaat atgattattt tatcggaaag catggccaaa aaatatttct
    12361 cagacgaaaa tccgatcgat aaagtgctgc ggcttgacgg taaaaccgat tacaaggtca
    12421 tcgccgtgta taatgatgtc cccggcaacg cgcatttgca tttcgatttt ttggccagcc
    12481 tcaccaaccg tgaggaggga aaaaacacgc aatggctaag caataatttt tcaacgtaca
    12541 cacggttaaa gcccggcgca tccacaaccg gcgtcgaatc caagatgcgt gaaatggtca
    12601 tgcgttacgg cggacctgag ttgcaacgat atgctaatat cacctttgaa caattttttt
    12661 cgcaaggcgg cgcatacgct tatcatctgc agccgatccg cgacatccat ttgtattcca
    12721 atcttgaaaa cgaacaagaa cccggcggcg acattacgta cgtttatatt ttttcaggta
    12781 ttgccatttt tattttgctc gttgcgtgtg tcaattttat gaatcttgcc accgcacgtt
    12841 ccgcaggtcg cgccaaagaa gtcggcattc gcaaagtgct cggctccgaa cgcggtcaac
    12901 tcatacgtct ctttctgatc gaatcgctgg tcatcacaac cttggcgatg atcatcggcg
    12961 tacttttggc tgaaatggca ttgccatttt ttaacgaact ctcaggtaaa tcgctgcata
    13021 tcggaatcgc gaccaatcct atgcttggta tcggacttgt tttggctgta cttttagtcg
    13081 gtgtactggc cggcacctat ccggcgtttt acctcacggc ttaccaaccg atcgctgtac
    13141 tcaaaggaac actcgcttcc ggcgtaaaaa atagcgggct gcgtaatgtc cttgttgttt
    13201 ttcaattctg cgtatccatc atcctgatcg cgggcacgat catcgtttat caacagctgc
    13261 aatatataca aaaccgaaat ctgggctttc aaaaagaaca gatcctcgtt attcataatg
    13321 tatggcaaat gaataaacaa ggtgatgcct tcaaacaatc cgtcctgcgc acacccggcg
    13381 tcgcaagcgc atcgttgacc agtactttac ccggacatcc taccggcaac agcgcatttc
    13441 agatcgaagg cgacgcgtcc aacgaaccct atctcctttg gcaagtgcga accgattttg
    13501 attttctcaa aactatgggc attgacatgg cccaaggccg tgatttctcg atcgaacatc
    13561 cttcggactc caccgcgatc attatcaatg aagccgctgt ccgtctgatg aatatgccgc
    13621 aacccctcgg caaacgcctg acgcgtttta gttcagcgcc gtacgaaatc gtcggcgtca
    13681 cgcaggattt caattttcaa tctttgcgca atccggttcg cccgatttcg atttttatga
    13741 atcctccggg ttttccggcg ccttcatttt tagcggttcg tgtaaaaccg gatcatttac
    13801 aggagaccat acaatccatc ggccaggtgt ggaatcaatt ccagcccggt gaaccgtacg
    13861 tgtacacctt ccttgatgaa gatttcggga aactgtatcg cacggaagag cgcgccggta
    13921 cgatcgtaac gacattttca agcttggcta tatttattgc ctgccttgga cttttgggtc
    13981 tcgccgcttt tgccaccgaa cggcgcacta aagaaattgg tattcgcaaa gtgctcggcg
    14041 catccgaagg tcacatcatc ggcatgatct gcaaagaatt cgtcatactg gttactgtcg
    14101 ctttagtcat ttccgtgccg atcgcatact acgtgatgaa tcgttggctg caggattttg
    14161 cctaccgcat tgacatcggc gctgatgtat ttattctgtc gggcgtgatt gcattgaccg
    14221 tggcgctgct tacggtaagc tacacggcga tccgcgccgc cttatccaat cccgtaaccg
    14281 cattgaaata tgaataacgt ttcgtccata gaaatacgcg cgatcgaaaa tcgcgacaat
    14341 ccggctttag catccatcat acgcacggtt atgccggagt ttggcgccga cggtccgggt
    14401 tttgccatac acgatgccga agtcaatgat atgttcgccg cttacacacg cccacgcagc
    14461 gcttatttcg tggtcgtagc cgatggcgta gtctccggcg gtggcggcat cgctccttta
    14521 gcgggcggag atgcgaacgt atgcgaatta aaaaaaatgt attttcttcc cggcttgcgg
    14581 ggtcgcggcg tcggacataa gatcatcacg ttttgtctgc aaagcgccat ggctttcggt
    14641 tataaacaat gttatctcga aacattgacc ggcatggacg cagcacaaca tttgtataaa
    14701 cagcacgggt ttcgttccat tcctcaagcg atgggacaaa ccggccacta cagttgcgat
    14761 cgtttttata tcaaagatat ttgaccgcaa tgccataaaa aaaccgaaag gacgcatcat
    14821 ggctgaagtc gttatccaat tgccggaact caactccaaa gaccgcatcg aagtcgaatg
    14881 ccggatcaac ggaaaaaaac aagtactgca ttaccgcgtg gaaatttttg cgtgggaaga
    14941 ttgcaatgaa cccgtagcgg atcgtgcact gtgcctcaaa cgattggttg aaaattacga
    15001 tcataattgg caacttcagc agatcggcgc ttatacggat aaaaccgtac aggtgatgtt
    15061 ccgcgaaaag cacacacccg gcactttaat ccattgatag aggcatcatg atcactctga
    15121 aaaacatcga aaagtattac gaaaacaaaa gcatcaaaaa ttatgtcctc cgggatatca
    15181 acctgaccat tcagcccggc gaatttgttt ccattatggg gccgagcggc tccggcaaat
    15241 ccaccctgct gcacatcctc ggcatgctgg acgaaccgac caaaggcgag tatctttttt
    15301 tggatcagga agtgcaccgc atatccgaac gccaaaaaac cgagttgcat aaaaatcaca
    15361 tcggatttgt ttttcagagt taccatctta tcgacgaact gaccgtctat gaaaacatcg
    15421 aaacacctct gctgtacaaa aaaacgagcg gctcggaacg caaagctttg gtcgcggata
    15481 ttttggatcg ttttaatatc gtcggtaaaa aggatctatt tcccagccag ctatccggcg
    15541 gtcaacaaca gcttgtcggc attgcccgtg cggtgatcac cgcaccgaaa gtcatcctcg
    15601 ccgacgaacc tacgggcaat ctcaattccg cccagggcga agagatcatg caacttttca
    15661 aaaaactcaa tcaggaaggc acgacgatca ttcaggtcac ccactctgaa aaaaatgcgg
    15721 cgtacggaaa tcgtatcatc aatcttttag acggattcat tcagaaataa tacttcgctg
    15781 ttacacattc ttattttttt atgttttgga aaatcaaagc gcttctgtat gccaggttcc
    15841 gacatgtatt tccttttggg tattttttaa ataaagagaa tgcggctttg ataaagcttc
    15901 tccaaccgac gattcgaccc gatgactatg tactggatgc cggatgcggc gatggcaatg
    15961 ctacgcgaat gcttttgaaa atacaccctc aagttcgaat catgggtata gatcaatctc
    16021 catccatggc aaaccctata gaacattccg gacgcactga aattcgaatc gcttcactgg
    16081 atgccatcgc agaagaaaat catacttttg atgccgtgat gtgtatcggg gtcaccgagt
    16141 atattgacga tatcccaagg gtactgcttg aaataaaacg tgcaaccaaa cccggtgcgc
    16201 cgatggtggt aacgtttgcc ccgcctgtgt tttggaatta tctgcgcttc ggatacggaa
    16261 agactctacg tttaatatca acgacggaag caactcactt tcttcaaaaa annnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngataaaacc tgaaatcgaa taagttcgga
    16441 gttcgcttcg aatacctgaa ttttgtaatt ttgaaacgtg atggatttgt tttcgttgat
    16501 gtcatattga aatatctggc tgaataccgg ttcataatac ccttgattga tgcgaacgac
    16561 gtattcacga tagagtatct gaataacatt gtgattaata cccgcataaa tgagctcgta
    16621 acgtgaaccg tgatgaatgg tattgatctg tagcggatct gtcgtcctac cggtatccgg
    16681 tgctgtggct tttttgtatc cgctctcccg gtagatcatc gtactaccga tattcgtaga
    16741 gtattgagtt ttattatcat atcctttata ggaaaaaggg atataagaag tgcatgatac
    16801 catggttaac aaaattgata aaaaaccgat atttcctgcg atacgacgac gcatagatga
    16861 tttcccttca cgatttggtt gttgttgttg gattaacata aattcaccaa aaatcgcaag
    16921 gtaaacattg ttatttaagc atttacaatg tatgccgtaa aaatttataa aattttttaa
    16981 ctccatgtcc gatttccgat ccgtcattcg tcattaatat atagtacatt taatacatac
    17041 caacgaaagg gtacaacgta tgcaatcgta catgatgatt ttccgcggtg gcgatgacgc
    17101 catggtcaaa atgtcaccgg atgagatcca gcagcacatg ggccagtggt ttgcttgggt
    17161 ggaaaaactg accaaagaag gacgttatgt cagcggcgaa ccgctggtta tggaaggcaa
    17221 agtcgtccgc gccaaaggcg tcatcacgga cggacctttt gctgaaggga aagaattggt
    17281 cggcggattc tttattgtca aagccaaatc catcgaagaa gcatgcgaca tggccaaagt
    17341 ttgccccagc tttcagtttg acggcgccac ggtcgagata cgccaggttc aggaaatgga
    17401 gcacgcataa taacccagca tgagtgtcgg cgaaaatcat ccatccatag agtctttggt
    17461 agaccatctt tttcgtaatt cggcgggaaa gatggtctcc gttttgacgc ggttattcgg
    17521 cagtcataat atcacgttgg ccgaagacgt cgtgcaggat acgctgctca aggcgatgca
    17581 aatctggccg cgcgccggta tcccgcaaaa tcccgaaggc tggcttatgc aggtagccaa
    17641 aaaccgtgcg ctggatctga tccgtcgccg cagccgcgaa atcgaattac cggaagatac
    17701 cgacccgctg ttatccagtg catggacccg tgcaacgcgt atggaatatc tttttctgcc
    17761 gcacgaaatc cgcgacgacc aactccgcct gatgtttacc tgctgccacc cgatgttgcc
    17821 acaggaaagc cgcatcgctc tgacgctcaa aacgctgtgc ggtctgggca ccaaagaaat
    17881 cgcacgcgcg ttcctgactt ccgaagaaac gattcaaaaa cgactatacc gcgccaaaca
    17941 gcttttgcgg gaacatcgta ttcggtttga aatccccgat gacgggatat cggaacgctt
    18001 caactcggtt ttgcatgtga tctatctgct gtttaatgaa ggctataata cgacccgcac
    18061 cgatgcggtg attcgccaag atctgatgga cgaatcaata cggttggggg aaatgctttt
    18121 acaaaataat cgcaccaata cccccgccac gcaggcattg ctagctctga tgtacctgca
    18181 cgccgcacgt accgatgcgc gtaccgacgc caccggccat attcttttg
//
LOCUS       DAOQGF010000066        18176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1439_length_18176_cov_7.513437, whole genome shotgun sequence.
ACCESSION   DAOQGF010000066 DAOQGF010000000
VERSION     DAOQGF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_18176_cov_7.513437"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PK937_09955"
     CDS             complement(<1..352)
                     /locus_tag="PK937_09955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH32936.1"
                     /translation="MIWKNETYVIDTDPKNLDVDVIHDFLGASYWAKDIPRSVVEKSI
                     RHSLCFGIYHETKQIGFARVITDYATFAYLADVFVVASHRGKGLSKWLMGCIQSHPEL
                     KNLRRWMLATRDAHG"
     gene            complement(381..1007)
                     /locus_tag="PK937_09960"
     CDS             complement(381..1007)
                     /locus_tag="PK937_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNH32937.1"
                     /translation="MFIPKRYRITDIDTIRAFVAEYEFGTLFSQSAGKPWATHIPMQW
                     FSDGSPHGVLRTHISKANSQWKDMEGQTVLAVFLGPHVHITPTWYDHVNVPTWNYASV
                     HITGRARIIEDPKAIREMLTVLVDTYESAHGNTYSVQSLPEDYYNREVQGLVGIEIQI
                     ENVDAAFKLSQNRHRSDYENIICQLRNSNDENSHRIADLMEKHTPEFS"
     gene            complement(1011..1937)
                     /locus_tag="PK937_09965"
     CDS             complement(1011..1937)
                     /locus_tag="PK937_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH32938.1"
                     /translation="MSSKKYFFSGLMSYVIWGFVPIFLKKLQGFDDYEIIFYRILLAA
                     LAMTGVAILLWGETSQAIGGLYRRSKKDFLHMLILTSIGGGLLATNWVTYVYVVNHVS
                     INAASFSYLILPIATAFLALILLKEKLNAMRWAAVAMSGVSCYLMANVDARQMAYIAA
                     ITLSYSFYLITQRKNTYMPRRTNLALQMIVGTALLLTMDPIHTAPIDLPMHFWINVTI
                     IAVVFTVTPLLLNLYALNGMGSSQLAFLIYINPIISFVTAIVWYGEDLNELAVISYSL
                     LAVAILLFNWEIIVKAAEKTGLRAATKTITAE"
     gene            2705..2899
                     /locus_tag="PK937_09970"
     CDS             2705..2899
                     /locus_tag="PK937_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32939.1"
                     /translation="MTSKVDSYDSVGVGVINGLKIVFTSWEDVFGVALEKTPKKNRSS
                     RLNKWRKTWNRLQNFPSLTS"
     gene            complement(3235..3744)
                     /locus_tag="PK937_09975"
     CDS             complement(3235..3744)
                     /locus_tag="PK937_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32940.1"
                     /translation="MKKIIFFLLLTYAASAFSQDLGWQRVRLICGSAWSFTSGDEYLY
                     TAIQIPVTKKIFVGLCGGYRPQDVKTVHADFTVGYDLVTSGPNRLFFKTDGSFFVREG
                     SFIFDARFFRQTIGLGYTSFILQKIVLTTEVAILGFYYGSRKDPPPSQKHPSVFGAGE
                     LKIGIGYKF"
     gene            complement(3741..4271)
                     /locus_tag="PK937_09980"
     CDS             complement(3741..4271)
                     /locus_tag="PK937_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNH32941.1"
                     /translation="MKKLILVLLFSSISLQAVAQENNRERPNIIIGGDVSPLSRGSGT
                     EDHFFIAGNYPINQKIHVGLSYGFLAKDAVSIQHEKLHQIGSALGYRLGQSGSFLFFA
                     KTELAFFYKPDVTKRLDKSFFRHTVGGGVWTRLMNRFFLNTEINIGGFFYGRNEPVKY
                     QFYNAADIKTGIGFQL"
     gene            complement(4276..5208)
                     /locus_tag="PK937_09985"
     CDS             complement(4276..5208)
                     /locus_tag="PK937_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32942.1"
                     /translation="MKKIIICLFFPLAMSCQSCYKEYYTDSRLQEEKDIVLSVLAPDT
                     SNGFIRENAIEVFVGKIVPTDIHNIYTVYGMQDSLLKELMINTRQFYIAYYKYDPNAI
                     VSIEGNGQNVILEYTDRGIYRDINRSLKIKPSARYKLKVEKKDGRVFTSETVVLNTLS
                     IQNDFSDTVLVKPYLEAGLFYADTVLLKSSFLGKAIYYVFQEKSNNFSFSVSTYTFQN
                     NAYDAVIFTKNEFDMTRIDFVTVNYETRAIDSAYGMFHQPHGHSMTPEFGDWDITLYD
                     IPIEKRSNIKGKNVVGVFGNYSADRKKVTYKADW"
     gene            complement(5274..6473)
                     /locus_tag="PK937_09990"
     CDS             complement(5274..6473)
                     /locus_tag="PK937_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32943.1"
                     /translation="MKSDKGNLSRQDAHLYLENLSKKIALNGNAGSLSKSLAVTGAPF
                     LAKIAKQGHVAINEVTDVSSVITVENQLHEIRMEVFNPQKAQAMSKSAAVTSGDLYGV
                     YADPIDYYDGSSDHVNFPVQIVSLNNGEYSSREEIWTAPYSADMDQIDDEFNQYISAL
                     RENTPYPLYALTIENIEEDPQDGNLNKSAAAGPYLGFEGVYLKVERDGLTDEEFELWW
                     TEPALSGLIHKRDMFNGHHHTDESGANVYFPDVNHKGNWEQLGDEFTTEIRLISLADG
                     IVKALTPWEDDATAGSMKRNSRSSMYNPVIDGVQTGAWADVKVYDDAFNCETNMVDDN
                     VGLHYYVDGFPESKDEDDPYSEGAVYRISAASIANRAPNGVRFETNQALNHKLDDINY
                     RFNYYQD"
     gene            6779..7699
                     /locus_tag="PK937_09995"
     CDS             6779..7699
                     /locus_tag="PK937_09995"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNH32944.1"
                     /translation="MELKSRHLLGLKGMSAEEIELILETALSFKEVLNRPVKKVPTLQ
                     GFTIANLFFEPSTRTRISFELAQKRLSADTVNFTPSGSSTSKGETLKDTARNIEAMKV
                     DMVVIRHSAAGAAWFLSQRLQTNVINAGDGAHEHPTQALLDILTLREKYGSLKGLRVT
                     IVGDITHSRVAKSNIYALKTLGAEVAVCGPPTFIPRNMADLGVRIYDNIHDAIRHSDV
                     LNVLRIQLERQSSGLFPSIREYHARFGINKEKLALGSKHMTIMHPGPINRGVELDSEV
                     ADGEYSVILDQVLNGVATRMAVMYLLSVTK"
     gene            7780..8790
                     /gene="rfbB"
                     /locus_tag="PK937_10000"
     CDS             7780..8790
                     /gene="rfbB"
                     /locus_tag="PK937_10000"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNH32945.1"
                     /translation="MQSILVTGGAGFIGSNFVRYMLKKYPSYRIIVLDALTYAGNRAN
                     LTDLEKNPRFFFYHGNICDRSVVDNLVSNVDAVINFAAETHVDRSIHEADSFAQTDVL
                     GTMVLLEAAKKYNIARYLQISTDEVYGSIENGEFTEEHSLSPNSPYSASKAGGDMMVR
                     AYHKTYGVPTLITRASNNYGPYQYPEKLIPLFVTNALDNIPVPLYGDGMNVRDWLYVE
                     DHCSAVDYVLHHGVIGEVYNIGGGNERTNVTITHTILKALNKPESLIKRVADRPGHDR
                     RYAVDTSKVRRLGWKPAYNFEEAIVETIQWYVKNESWWRAIKEKQKAFQEFMKKNYED
                     RK"
     gene            8792..9274
                     /locus_tag="PK937_10005"
     CDS             8792..9274
                     /locus_tag="PK937_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH32946.1"
                     /translation="MADNAHKTLEDLIKSVEIFNGLDTRDINKVLKVATGKKFETDAV
                     VFREGDQGDCFYLIIEGKVCVSKSVSNDKIEEVAILSAGDYFGEMALFDGEPRSASVV
                     AIESTKLLEVKNSQFIKIIMSDENFSRKVLWAFCSTFAKRLRATNHLLSIFAKNQTPR
                     "
     gene            complement(9252..10142)
                     /locus_tag="PK937_10010"
     CDS             complement(9252..10142)
                     /locus_tag="PK937_10010"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNH32947.1"
                     /translation="MTVRLILFFSLTISLHAGSGETGFAFLKINASARSSAMGEVSTY
                     DGQDPMGVLVNPANLSVTPSHSVAFTYNRWIESTDYQAFSGKFKTGSWAWSLYYLYTG
                     VDDIPQRDIPSEEPTAYFSSHDLAIGFVLSRALNDRWFAGIGTKYIYERIQNTVTAWA
                     WDGGLYYVPNETWRAGFSFQNLGRSGKLIDERIRLPFQVRLSGGYTVYRQENRHRADL
                     MADVIKTYGASPRLHTGSEYVFQEKVCIRLGYVWGYASRNISAGVGLIFQRRMRLDYA
                     WTPFTNSLGSSHRISAGFDF"
     gene            complement(10144..11739)
                     /locus_tag="PK937_10015"
     CDS             complement(10144..11739)
                     /locus_tag="PK937_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH32948.1"
                     /translation="MQRLKGNSLFSGLPETEIAKLEEFIRIKDFPKNAVLIDEGVPGS
                     YLYLLIAGNVKITRNGLDGREMILTERHAGDFFGEMALLDDAPRSARVVATEDSVAGV
                     IGKNEFNAMMIREPRVAVNILRTISMRLRLSNDQIMQSMEEKERYHAKQLERFQSLIE
                     FNKTLGAEREEVGLFRVVPVIVRSQILCHDVLLVLDDPESDKYHTSIQEVSGWRMIDF
                     SETDKGTAKLAALKKTLTMEDFAAYEFRDTMEPQAFWKNAASIMIAPINGPRQNHGFI
                     AVKSAQKFRWDAEDEAFMTTLASDVSMVLRNMRLMRKAMFDEKMSAVGKASSSLLHDF
                     KNLLSVVYNYAKLIKTAATEEERKELVEKIVKFSSLMMGMSQEVLIYGDGSVKASPTP
                     VRISEIFDMVMPLLDTAGKKITMQTSIPEGMEFPLDKDKICRVFYNILTNACDAMGDA
                     GHISVTAAFEKNDLVIRISDTGPGIPEDVLPQIFKPFVTTKKHGTGLGLPIVKSIVEA
                     HRGKVQIETRIGSGTTFIFTFPK"
     gene            complement(11742..13244)
                     /gene="dacB"
                     /locus_tag="PK937_10020"
     CDS             complement(11742..13244)
                     /gene="dacB"
                     /locus_tag="PK937_10020"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNH32949.1"
                     /translation="MRLMFLWCILGIVWSPDLKSQSKKQNGSPAIKQLQADVLDDIGQ
                     KFVENASWGIVVQSLKNGETLVSVNPKKNLIPASNRKIFSSIFALDQLGADFTYVTQV
                     FAKGVLDSDSVFKGDIIIRGAGDPTISGRFYENKITKILEDWADSVQRKGIKSIRGRI
                     IGDDNGLMGDDVLGDYWESTEESYWYSAQISGLNFNDNCVDWYAKPTRIGEPAKIILI
                     PPTQYIKINNFVKTVSSKSEAVKVDAIRRRAANEVDFIGSILTESDPQHGYVTVENPT
                     RYTAHVFSEILQSRNIRVDSAAVDADDLVRYVYNETDSNMIRIARYVSPAMPELMKII
                     NKNSHNLYAELLWRTVAAVKDSSGTSKRALALEEKFLKSVGLNPDKISICDGSGYARG
                     NLVSPQDIVTLLTWVRKHKNWKTFYESLPLAGEEGTVKSRMKGTLAEGNARAKTGYLD
                     NVRTISGFVKTRDGEELVYSVMCNNFTADRLEIDKIQDMIIGRLAFFTRN"
     gene            complement(13260..14537)
                     /locus_tag="PK937_10025"
     CDS             complement(13260..14537)
                     /locus_tag="PK937_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNH32950.1"
                     /translation="MKSFHRRSYLTVIATLCFALPTLGQDIYTTGTPFNLYFTPFAIH
                     VGDFNRDGFMDVVTSGKNDLDGSGIISVLYGKGDGTFKNKIDLASEKFAVDIQVGDLD
                     NDGMPDIVTANGPAQTVSVFMNQKSGGFKAKDPIKIKGEPSLINLGDYDKDGKIDIAI
                     VIKATKELHIYKGTSYKLSAVKILDDVPSAIRSDDYSGNGFSHMVISYEAASYVTLAN
                     MVELKGYKYEINTAKIDMLALPQFTHHADGNNDGFEDAFLFDRNKSTIAVIKAEANGL
                     ILDNIFTLKVPAGSTSFCVGDFNKDKKADIALLDSANAQVTIYMNQQPDPAPQNTFAQ
                     GKLIIVYAPDFTKPNNADVLMSSAYKKVSMYLYYPDGKPARKYFDIESDLSDGQFTIE
                     WAGTDENDAELPNGEYYFYYQLGSLIITRGLKK"
     gene            complement(14714..15976)
                     /gene="hisS"
                     /locus_tag="PK937_10030"
     CDS             complement(14714..15976)
                     /gene="hisS"
                     /locus_tag="PK937_10030"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNH32951.1"
                     /translation="MTYRMPTGTKDVLPSDSYKWQYVERKIADVMRRFNYKEIRTPMF
                     EYTELFARGIGEATDVVGKEMYTFLDKGQESLTLRPELTASVMRSYIENSLGEQLPLN
                     KCWYMGPMFRQERPQKGRLRQFHQFGVEALGSASPEADVETMLVVLTVYEELGIKALN
                     FKINSVGCPQCRPAYRDALVAFLDGVKGQLSEESRKRLVVNPMRILDSKDERDIALTQ
                     SAPLMKDHLCTDCATHFSRVQSSLNGLGIVYEIDGRLVRGLDYYTKTAYEIQSGNLGS
                     QNALGGGGRYDLLAEEFGGKPTPAVGFAAGIERLMIVLEAEGIPIGEPEKLDVYFIAL
                     GAAAVGATMPLIQKARREGLTCDTDLLARSMKAQMRDANRMQARFTVILGDNEIAQNM
                     AVVKNMSDGAQEQISLNELTSYLKTKLA"
     gene            complement(15995..17722)
                     /locus_tag="PK937_10035"
     CDS             complement(15995..17722)
                     /locus_tag="PK937_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH32952.1"
                     /translation="MLNETSALFNSRSDNRVMLWSPFLATLCVTLVMAIWNVITYQRL
                     FDLSPDESFFVSVTGFLVVGLTIVVGGMMHLALFRKTLTAESHSGTLSEESIRRIWVE
                     WLKYPRTMVFYLYFVHLAGMSLLLSSVLLVPFVIESISGWEYMYKTLIWVLLGLIPFS
                     IILFYVLEWRIHVCSSRFPEVLAQMPDANDPDIVWIRLRTRVMAPILAMTLWYGLLLY
                     NYHNHPVAITLLALVMFGFIGWLTTRTVQTAHDRWLTLWSGPDIYHTEEVGHTMLHAG
                     DIYRLQKTAERFAATAKREVLVLRDQIQPLGATAEHILSGLKSQNSYASNHASALAQI
                     TASLEELMKSVAQISENSKSVNQSTLKGSQEAEDGKRHVADTLQSMEQIHRESQTSAQ
                     RILDLSLKMTQIEDVIKIINYITDQTKILAFNAAIETAGAGEMGERFGVVAKEIRHLA
                     QNIADSTEEIKKILLDVRKASQVSVLASEKELMQVKEGIRHAGDARESFEQIYWMVRQ
                     TMESVQQIAQAIQQQQSALEQLWMSIKDIDAGAKESLRQNKNMLSSAEELRMLSQNTR
                     TSQRPQSTA"
     gene            complement(17724..>18176)
                     /locus_tag="PK937_10040"
     CDS             complement(17724..>18176)
                     /locus_tag="PK937_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH32953.1"
                     /translation="IIMVLHAPDLIRATKDFSVREQVQSIPKSARSILVVDDSLNTRE
                     IEKTILQAYGYDVETAKDGLDALEKIQQKKYDLVVTDLEMPAMDGLTLTSRIKSEASY
                     QHIPVVIVTSRDSAEDKRRGIEAGANAYIVKGSFDQTNLIDTVESLIG"
BASE COUNT         4975 a         4300 c         3849 g         5052 t
ORIGIN      
        1 gcccgtgtgc atcgcgtgtg gccagcatcc atcgtctcag atttttcaat tcgggatgac
       61 tttgaataca tcccatcagc catttggaca accctttacc gcgatgcgaa gcgacaacaa
      121 aaacatcggc aaggtatgca aatgtagcat aatccgtgat cacccgtgca aaacctattt
      181 gttttgtttc atggtagatg ccaaaacaaa gtgaatgacg tatggatttt tccacgacgg
      241 atctgggaat atcctttgcc caatacgacg cacctaaaaa atcatgaatc acatccacat
      301 ccagattttt cggatcggta tctatcacgt acgtttcgtt tttccaaatc attgttgcgc
      361 ccttagtaaa catttaaaat ttaagaaaat tccggcgtgt gtttttccat caggtcggct
      421 atccggtggg aattttcatc gttgctgtta cgtaattggc aaataatatt ttcataatcc
      481 gaccggtgac gattctgact caatttgaaa gcggcatcca cgttttcgat ttgtatttct
      541 atccccacca aaccttgcac ttcgcggttg taataatctt ccggtaagga ttgaacagaa
      601 tatgtattgc cgtgtgccga ttcatatgta tctaccaata cggtgagcat ttcacgaatg
      661 gctttcggat cttctatgat acgggcacgc cccgtgatgt gtacggacgc atagttccac
      721 gtcggcacat tgacatgatc ataccatgtc ggcgtgatat gcacatgcgg ccccagaaaa
      781 accgccaaaa cggtctggcc ttccatgtct ttccattgcg aattggcttt ggatatatgc
      841 gtacggagaa caccgtgcgg gctcccgtcg gaaaaccact gcatcggtat gtgtgtggcc
      901 cacggcttgc cggccgattg actgaataac gttccaaatt cgtactccgc cacaaacgca
      961 cgtatcgtat ctatatccgt gatccggtat cgttttggaa tgaacatacg ttactccgca
     1021 gtgatggttt ttgttgcggc cctcaatccg gttttttcgg cggccttgac gatgatctcc
     1081 caattgaaaa gcaaaatggc caccgcgagg agactgtatg agatcacagc cagttcgttc
     1141 aaatcttctc cgtaccaaac aatcgccgtg acgaaactga taatcgggtt gatgtaaatc
     1201 aaaaacgcaa gctgggacga acccataccg ttgagcgcgt aaagattgag taaaagcggt
     1261 gtcacggtaa aaacaaccgc aatgatcgtg acattgatcc agaagtgcat cggcaaatca
     1321 atcggtgcgg tatgtatggg atccatcgtc aaaagtaatg ctgtgccaac gatcatttgc
     1381 agagcaagat tggtacggcg cggcatatac gtgtttttac gttgagtgat cagataaaaa
     1441 ctatacgaca gcgtaatcgc cgctatgtat gccatctggc gtgcgtccac attggccatc
     1501 aaataacaac tgacaccgct catcgctacc gcggcccacc gcatcgcatt cagtttctct
     1561 ttgagcaaga tcaatgccaa aaacgcagtc gctatgggta aaatcaaata ggaaaacgag
     1621 gcggcgttga tactgacgtg attgactaca taaacgtacg ttacccaatt cgtcgccaaa
     1681 agtcctccgc cgatagatgt caatatcagc atatgcaaaa aatctttttt tgagcggcgg
     1741 tacaatcctc cgatcgcttg cgaggtctca ccccacaaaa ggatcgccac acctgtcatg
     1801 gccaaagccg ccagcaaaat gcgataaaaa attatttcgt aatcatcaaa tccttgcagt
     1861 tttttcaaaa aaatgggaac aaacccccaa atcacataag acattaaacc gctaaaaaag
     1921 tactttttgg agctcataat gaaatatagt ccaatttttt aattaataaa tagtccaatg
     1981 tatcattcgt ttgataaagg tagtccaata taggatacat ttgttccgtc aaaaagtacc
     2041 aatttttatg ttttcagtat agaccaatgt gtttttttga agaaaatgat caccctgttt
     2101 attcgattgt gatcacaaac ggaattcgga ataatgttct gaatgactat atccatcttg
     2161 tgtaggtaaa agtaataaac gtatagggtt tttaagctac ctcgcctttt catactttat
     2221 ttttgccgga aaagatattt tgagaaaaat gagggtgtaa cagttggctc aggcggcgat
     2281 ttctgagggg tgaaggtgga attgtcacaa aatcatcgta tggactttta gttttggtca
     2341 tggtttttga atttgaagcg ggggctggtc atactaaatc caacggaagt ttctcggaca
     2401 tgaaaatcta ttcgctgcga tacgatgggc gtatcaagtc gttttctcct tttcatgcac
     2461 ttagttattt agatttttcc taccggcaca agaatgacga cggttttaga accgatatta
     2521 agcaggcaat tatcggagcc tcaatacatt tggtagaaac gaaagtatta atagtagcat
     2581 ctgatatttc gttttcaaaa tcttttgcag attatggaac tgcgaaacaa atagctttgg
     2641 cgtcggtaag actattttag cacgtgacaa taataatgtc ggtgggcttc ttaaggccgg
     2701 agtagtgacg agcaaagtgg acagctacga ctctgtcggt gtcggagtta ttaacggtct
     2761 caaaatagta tttacatcat gggaagatgt atttggggtg gccttggaaa aaacgccgaa
     2821 aaagaacagg agttcgcgac ttaacaaatg gcggaaaacg tggaacagac ttcaaaactt
     2881 cccatcgctg acgtcatagg aactgttttc cgccgccacg taagcgatct ccggttccgg
     2941 cgttttttcc acagccttga tttttctttt ggttcttttc tttgcatcaa ggcaaagaaa
     3001 agaacatggt taatttaaat actatttcaa gacaattaat gtctttggat aattgcgatc
     3061 cgaaagcggc gtaagtaatt agtcagagca tgtatcgtgg aagcgattgg tttcttctct
     3121 gttgcgtttt catcggatat tcgaatccac acacatcaag accacctgta aaacagaaat
     3181 aaccgcaaaa accaatcgca tacccgcctt caaccgtatc ggcgtttaac gatgttaaaa
     3241 cttataacct atgccaattt ttaactcacc ggcaccaaaa acggaggggt gtttttgact
     3301 tggcggtgga tctttccggc taccatagta aaaccccaaa atggctactt ctgtcgttaa
     3361 gactattttt tgaagtataa acgatgtgta gcctaatcct attgtttgtc ggaaaaaacg
     3421 cgcgtcaaaa ataaaactac cttcccgtac gaaaaaagaa ccgtctgttt taaaaaataa
     3481 ccggttgggt cctgatgtaa ccaagtcgta acccacagta aaatccgcat ggacggtttt
     3541 tacatcttga ggccgatatc ctccacaaag cccgacaaat attttttttg taacaggaat
     3601 ttgaatagcc gtatataaat attcgtcacc ggatgtaaat gaccacgcag agccgcagat
     3661 caaacgaact ctctgccatc ccaaatcctg cgaaaatgct gatgcggcat aggttagtag
     3721 aagaaaaaat ataatttttt tcataactga aaccctatac ctgtttttat gtcagcggca
     3781 ttataaaatt gatatttgac gggttcattg cgaccataga aaaaaccacc aatatttatt
     3841 tctgtattca gaaaaaaacg gttcatcagt cgagtccata ctcctccacc cacggtatgt
     3901 ctgaagaaac ttttatctaa ccgttttgtg acgtcgggtt tataaaaaaa agcgagctct
     3961 gtctttgcaa aaaaaagaaa acttcccgat tgtccaagac gataccctaa agcgctgccg
     4021 atttgatgga gtttttcgtg ctggatcgaa accgcatctt tagcgagaaa accataactc
     4081 aggccgacat gaattttttg gttaatagga taattaccgg ctatgaaaaa atgatcttcc
     4141 gtgccggaac cacggcttaa gggtgaaacg tcccctccga ttataatatt aggacgttcc
     4201 cggttatttt cttgagcaac cgcttgcaat gaaatactac tgaaaagtaa gacaaggata
     4261 agttttttca taaaatcacc aatctgcttt ataagttact ttttttcgat cggcggaata
     4321 attaccaaat acgccgacaa cattttttcc tttgatatta gagcgttttt caatgggaat
     4381 atcgtaaagc gtaatatccc aatcaccaaa ttcgggtgtc atactatgcc cgtgaggctg
     4441 gtgaaacatg ccgtatgcac tgtctatggc gcgggtttca taatttactg taacaaagtc
     4501 tattcgggtc atatcaaatt catttttagt gaatattaca gcgtcataag cattgttttg
     4561 gaatgtataa gtgcttaccg aaaatgaaaa attattggat ttctcttgaa acacataata
     4621 aattgcttta cccagaaaac ttgatttaag caacacagtg tcggcataaa acaaacctgc
     4681 ttctaagtaa ggtttgacca atacagtatc tgaaaaatca ttctgaatag ataaggtatt
     4741 tagaactact gtttcagatg taaagacccg accgtccttt ttttcaactt ttagtttgta
     4801 ccgtgcagat ggtttgattt tgaggctacg atttatatct ctgtaaattc cgcgatccgt
     4861 atattccaaa atcacattct gaccgttacc ttcgatggag acgatggcgt tgggatcgta
     4921 tttgtaataa gcgatgtaaa actgccgggt attgatcatc aattctttta atagcgaatc
     4981 ctgcatgcca tatacagtat atatattatg aatatccgta ggtacgatct tacccacaaa
     5041 aacttcgatg gcattttccc gaatgaaccc attcgatgtg tccggagcta aaacgctcaa
     5101 tacaatatcc ttttcttcct gaagacggga gtctgtatag tattctttat agcacgattg
     5161 gcaggacatc gccaaaggaa aaaacaaaca aatgattatt tttttcatat atgtctattt
     5221 ggttttaaat aaatcagtct ctcggcatct gattgattta aaattcgaaa atattaatct
     5281 tgataataat tgaaacgata attgatatca tcaagcttat gatttaaagc ttgatttgtt
     5341 tcaaatctta ccccattggg cgcccgatta gctatgcttg cagcagaaat acgatataca
     5401 gcgccttcgg aatacggatc gtcttcgtct tttgattcgg gaaagccatc aacataataa
     5461 tgtaatccga cattgtcatc caccatatta gtttcgcaat tgaacgcgtc gtcgtaaact
     5521 ttaacatcgg cccatgcgcc ggtttgtacg ccatcaatta ccgggttata catagagcta
     5581 cggctattac gtttcatact accggctgta gcatcatctt cccacggagt taaagctttg
     5641 acaatgccgt cggctaggct gatcagtcgg atctcagtag taaactcatc accgagttgt
     5701 tcccagtttc ctttgtgatt gacatccgga aaataaacat tggccccgct ttcatccgtg
     5761 tgatgatgac cgttgaacat atcgcgtttg tggatgagac ccgacaaggc cggctcagtc
     5821 caccacagtt cgaactcttc atcggtcaac ccatcccgtt ctactttaag atatactcct
     5881 tcaaatccaa gatacgggcc ggccgccgcg cttttattta aatttccgtc ttgcgggtct
     5941 tcttcgatat tttctattgt caatgcgtac aaaggatacg gcgtattctc gcgtagcgca
     6001 ctgatatatt gattaaactc gtcatcaatt tgatccatat ccgcggaata aggcgcagtc
     6061 catatttctt cacggcttga atactctccg ttattgagag atacgatctg aacagggaaa
     6121 ttgacatgat ctgaggagcc gtcatagtaa tcaatcggat ctgcatacac gccatataaa
     6181 tcgccggaag ttaccgccgc gcttttggac atagcttgcg ccttttgcgg attgaatact
     6241 tccattcgaa tttcgtgtaa ctgattttct actgttataa ccgaagagac atcggtcact
     6301 tcattgattg ccacgtggcc ttgcttagct atcttggcaa gaaatggcgc tcccgttacg
     6361 gctaaagact tagatagaga gcctgcattg ccattcaagg caattttttt agataaattt
     6421 tcaagataga gatgtgcgtc ctgacgggat agatttcctt tgtcggattt cacttcagat
     6481 gcggtttgat tggaattttc gcaggaagta aaaaacaacg caagaataag cgctaccccc
     6541 ccccccacat tttacgatgc tgattcatag attacctcat tagttaatat ctgactgccg
     6601 agagacttag taatacttag agttttttta taaacaacga tggcacatca tatatgatat
     6661 catattgaaa atcaagattt ttttgacatt ctttgccaaa ccaaaataga attgccttgc
     6721 taaaaacagc cttcccgcct atattgccgc gaaatttccg agattttgtg tgccttctat
     6781 ggagcttaaa agccgtcact tgctcggcct caaaggaatg agcgctgaag agatcgagct
     6841 gatactcgag acggctctgt cgtttaaaga agttctcaat cgccctgtaa aaaaagttcc
     6901 cacccttcag ggttttacca ttgctaattt gttttttgaa ccgtccaccc ggacgcgcat
     6961 ctcgtttgaa ttagctcaaa aaagattgtc agccgacacg gtcaatttta caccatcggg
     7021 cagcagtaca tccaaaggcg aaacgctcaa agataccgcg cgcaatatcg aggctatgaa
     7081 ggtagatatg gtggtgattc gccattcggc tgcgggcgcg gcttggtttt tatcgcaacg
     7141 tttacaaacc aatgtcatca atgccggcga tggagcgcac gaacatccga cacaagcgct
     7201 tttagatata ttgactctgc gtgaaaaata cggatctctc aaaggtttgc gcgtaaccat
     7261 cgtgggcgat atcacccaca gccgcgtggc caaatccaac atctatgcgc tcaaaacatt
     7321 gggcgcggaa gttgccgtct gcgggccgcc gacgtttatc ccgcgtaaca tggcggactt
     7381 gggtgttcgt atatatgata atatacacga cgcgattcgc cattcggatg tgctcaatgt
     7441 gctgcgtatc cagcttgaac gtcagtcttc cggactcttt ccgtccattc gtgaatacca
     7501 tgcgcgattt ggtattaaca aagaaaaatt agcgctcggt tcgaaacata tgacgatcat
     7561 gcatccggga cctatcaatc gcggcgtaga acttgacagc gaagtggccg acggcgaata
     7621 ttccgtcatc ttggatcagg ttctcaatgg cgtcgccaca cgcatggccg taatgtatct
     7681 tttgagcgtt accaaataga tttattaatg accaagcttg cactatctgt gtacaatagt
     7741 ttgtgcaaac ataattgcct tgttaggata taaaaaagta tgcaatccat tctcgtcacc
     7801 ggcggcgccg gatttatcgg aagtaatttt gttcgctaca tgcttaaaaa gtacccgtcg
     7861 taccgtatca tcgtgctcga tgcgcttacg tatgccggta atcgcgccaa tcttaccgat
     7921 cttgaaaaaa atccgcgttt ctttttttat cacggaaata tctgcgaccg aagcgtcgta
     7981 gataatcttg tatccaatgt ggacgccgta atcaatttcg ccgcggaaac tcatgtggat
     8041 cgttctatcc atgaagcgga ttcttttgca caaacggatg ttttgggcac tatggtattg
     8101 cttgaggcgg ccaaaaaata caacatcgcg cggtacctgc agatttccac tgacgaagtt
     8161 tatggttcaa ttgaaaacgg cgaattcacc gaagaacatt cgctttcacc caatagcccg
     8221 tattccgcca gtaaagccgg cggcgacatg atggtgcgcg cttatcataa aacgtacggc
     8281 gtcccgactt tgatcacccg cgcatccaat aactacggcc cctatcaata tccggaaaaa
     8341 ctgattccgc tttttgtaac caatgctttg gataacattc ctgttcctct ctatggcgac
     8401 ggcatgaatg tgcgcgactg gttatatgtc gaagaccatt gctcggccgt ggattatgtg
     8461 cttcatcacg gcgtgatcgg cgaagtctat aatatcggcg gcggtaacga acgcaccaac
     8521 gtcaccataa cacacacgat cctaaaagct ctgaataaac ccgaatctct gatcaaacgc
     8581 gtagccgacc gccccggcca tgatcgccgt tatgccgtgg atacctccaa agtgcgtcgt
     8641 ctgggttgga aacctgctta caattttgaa gaagccatcg tcgaaaccat ccaatggtat
     8701 gtgaaaaatg aatcctggtg gcgcgctatc aaagaaaaac aaaaagcatt tcaggaattc
     8761 atgaaaaaga actacgaaga tcggaaataa tatggccgac aacgcacaca aaacactgga
     8821 agacctcatc aagagtgtcg aaattttcaa cggattggat acccgcgaca tcaacaaagt
     8881 tctaaaagtc gctaccggga aaaaatttga aaccgatgcg gtggtgtttc gagaaggcga
     8941 tcaaggtgat tgtttttacc tgatcatcga aggaaaagtg tgtgtttcca aaagcgtgag
     9001 taacgataaa attgaggaag tcgccattct gagcgccggt gattatttcg gtgagatggc
     9061 gctttttgac ggggaaccgc gttccgcgtc cgtagttgcg atcgagtcca ccaaacttct
     9121 ggaagttaaa aacagtcagt ttatcaaaat catcatgagc gatgaaaatt tttcacgcaa
     9181 agtcctgtgg gctttttgtt cgactttcgc caaacgcttg cgtgcaacca accatctgct
     9241 gagcatcttc gctaaaaatc aaaccccgcg ctgatacggt gtgatgaccc gagactattg
     9301 gtaaagggcg tccaagcata atccaaacgc atacgacgtt gaaagatcaa accaacaccg
     9361 gcgctgatat tgcgcgaagc ataaccccaa acgtacccca accgaataca taccttttcc
     9421 tgaaacacgt attcacttcc cgtatgcaat cgaggcgatg caccgtaggt tttgatcaca
     9481 tcggccatga gatccgcgcg atgccggttt tcctgacgat acaccgtata accacccgat
     9541 aatcgcactt gaaacgggag tcttatgcgt tcatcgatta acttgcccga acgtccaaga
     9601 ttttggaaac taaaaccagc gcgccatgtt tcattgggca cataatacaa tcccccgtcc
     9661 cacgcccagg ccgtaaccgt attttgtatg cgttcataaa tatatttcgt accgataccg
     9721 gcgaaccaac gatcattgag agcgcgggac aacacaaaac cgatcgccaa atcgtgcgaa
     9781 gaaaaatacg ccgtcggttc ctccgagggt atatcgcgct gaggaatatc atccacacct
     9841 gtgtacaggt aatacaatga ccacgcccaa gatccggttt taaatttccc cgaaaacgcc
     9901 tgataatcgg tgctttcaat ccaccggtta tacgtgaagg ccacactatg ggatggtgtc
     9961 acggaaagat tggccggatt taccaaaacc cccataggat cctgaccgtc gtacgtgctc
    10021 acttcgccca tcgccgagga acgggcgctg gcattgatct taagaaaagc aaatcccgtc
    10081 tcgccggatc cggcatgaag tgaaatcgtc agcgaaaaaa ataatattaa acgcactgtc
    10141 attttatttg ggaaatgtaa aaataaatgt tgtaccgctg ccgattcggg tttcgatctg
    10201 aacttttccg cgatgcgctt cgacaatgga tttgacaatc ggcaaaccca agcccgtacc
    10261 gtgttttttg gtggtaacga acggtttgaa tatttgaggc aatacatctt ccggtatacc
    10321 cggtcctgtg tcggaaatac gaatcaccaa atcatttttt tcaaaagcgg ccgtaacgga
    10381 tatatgcccc gcatcgccca tcgcatcgca ggcattggta agtatattat aaaaaacacg
    10441 gcagatttta tctttatcga gaggaaattc cataccttca gggatggatg tctgcatagt
    10501 gattttttta ccggcggtat caagcaacgg catcaccata tcaaaaattt cgctgatgcg
    10561 taccggtgtg ggactggctt tgacggaacc atccccgtaa atcaacacct cctgcgacat
    10621 tcccatcata agcgacgaaa atttcacgat tttttctact aattcttttc gctcttcttc
    10681 cgtcgcggct gtcttgatca gtttcgcata attataaaca accgacagca ggtttttaaa
    10741 atcatgcaac aacgaactcg acgctttacc gacggcggac attttttcat caaacatcgc
    10801 tttgcgcatc aggcgcatat tgcgtaacac catagataca tccgacgcca gcgtagtcat
    10861 aaaggcttca tcttctgcat cccatcgaaa cttttgcgcg gacttcacgg caataaaacc
    10921 gtgattttga cgcgggccat tgatcggtgc gatcattata ctggccgcat ttttccaaaa
    10981 cgcttgcggc tccatcgtgt cacgaaattc gtatgcggca aagtcttcca tcgtcagtgt
    11041 ttttttaaga gccgctaatt tagccgtgcc tttatccgtt tcactaaaat cgatcatacg
    11101 ccagcccgac acttcttgaa tgcttgtatg atatttatcc gactcgggat catccaatac
    11161 taagagtacg tcatgacata atatctgact gcgaacaata acaggcacaa cacgaaacaa
    11221 tccgacttct tcacgttcgg cgccgagtgt tttattaaat tcgatcaaac tttggaagcg
    11281 ctccaattgt ttggcatgat agcgctcctt ctcttccatg gattgcataa tctgatcgtt
    11341 ggacaagcgc agacgcatgc tgatcgttcg tagaatattg accgctaccc gcggttcacg
    11401 gatcatcatc gcattaaatt cgtttttacc gatcacaccg gccaccgaat cctccgttgc
    11461 aacaacccga gccgatcgcg gtgcatcatc gagtaacgcc atttcaccga aaaaatcgcc
    11521 ggcatgccgc tcggtaagta tcatctcgcg accgtccaat ccgttgcgcg tgattttaac
    11581 attgcccgcg atcaacagat acaaataact tcccggcaca ccctcgtcaa tcagtaccgc
    11641 gtttttcgga aaatctttaa tacgaataaa ttcttcgagt ttggcaattt ccgtctcggg
    11701 caaacccgaa aaaagactgt ttcctttcag tcgctgcata tttaattcct tgtaaaaaag
    11761 gcaaggcgtc cgatgatcat atcttgtatt ttatctattt cgagacgatc tgcggtaaaa
    11821 ttattacaca tgacactata caccaattct tctccgtcgc gcgtttttac aaaacctgaa
    11881 atagttcgga cattatcaag atatcctgtt ttggcacgcg cattaccttc ggccaaggtt
    11941 cctttcatgc gcgatttgac ggtgccttct tcaccggcca aaggcagtga ttcgtaaaaa
    12001 gttttccaat ttttgtgttt gcgaacccac gtcaataatg tgacaatatc ctgaggtgac
    12061 acaagattgc cgcgcgcata acccgatccg tcgcaaatac ttattttatc cggattcagt
    12121 ccgacggatt ttaaaaattt ttcttccaac gccaacgcgc gtttggaagt tcccgacgaa
    12181 tctttcaccg ccgccaccgt acgccataat aactcggcgt atagattgtg actgtttttg
    12241 ttgattattt tcatcaactc gggcatggcc ggtgaaacat accgtgcaat acgtatcata
    12301 ttggaatcgg tttcattgta tacatacctc accaaatcat cggcatcaac cgcagcgcta
    12361 tccacgcgta tgtttctgga ttggaggatt tcactaaata cgtgtgccgt gtagcgtgtc
    12421 ggattttcta cggtcacata cccgtgttgc ggatcgctct cagtgagaat actacctata
    12481 aaatccactt cattggcagc gcgtcgccgt atcgcatcta ctttgaccgc ttccgatttt
    12541 gacgagacgg ttttaacaaa gttattaatt ttgatatact gcgtcggcgg tatcagaata
    12601 atcttagccg gttcgccgat acgcgtgggt ttggcatacc aatccacgca gttatcgtta
    12661 aaattgagtc cgctgatttg tgccgagtac caatacgatt cttccgtaga ttcccaataa
    12721 tccccgagca catcgtcgcc cataagaccg ttatcatcgc cgatgatgcg accgcgaatg
    12781 gatttgattc cttttcgttg tacgctatcc gcccaatctt ctaaaatttt tgtgatctta
    12841 ttttcataaa accgtccgga aatcgtcggg tcgcccgcac cgcgaattat aatatcgccc
    12901 ttgaaaaccg aatccgaatc cagtacacct ttagcaaaaa cctgtgtgac ataggtaaaa
    12961 tcggcgccca attgatccaa cgcaaaaatg gaggaaaaaa tttttcgatt ggatgccggg
    13021 atgagatttt ttttggggtt tacggaaacc aacgtctcgc cgtttttgag cgattggacc
    13081 acaattcccc acgacgcatt ctcgacaaat ttttgtccga tgtcatcaag gacgtccgct
    13141 tgcaactgtt tgatagcggg cgaaccattc tgttttttgg attgtgattt gagatccgga
    13201 ctccacacga tgccgaggat gcaccataaa aacatcaatc gcatgctgtt catttctctt
    13261 tattttttga ggccgcgtgt tatgatgagg ctgcccagct gatagtagaa gtagtattcc
    13321 ccgttaggta attctgcatc gttttcatcc gtaccggccc actcgatcgt aaattgaccg
    13381 tcggataaat cagattcgat atcaaaatat ttacgtgccg gctttccgtc cgggtaatag
    13441 agatacatac tgactttttt gtacgcagac gacatgagga cgtcggcatt attgggcttg
    13501 gtaaaatccg gtgcatacac gatgatcagt tttccttgcg caaaagtatt ttgcggcgcc
    13561 ggatcaggtt gctgattcat ataaatcgtt acttgcgcat tggccgaatc caaaagcgca
    13621 atatcggctt ttttatcttt gttaaaatcc ccgacacaaa acgacgtcga accagcagga
    13681 actttgagtg taaatatgtt atccaaaata agaccattgg cttctgcttt gatcaccgcg
    13741 atcgtacttt tgttgcgatc aaaaagaaac gcatcttcga atccgtcgtt attaccgtcc
    13801 gcatggtggg taaattgtgg taaggccaac atatctattt tggccgtgtt gatttcgtat
    13861 ttgtaacctt tgagttcgac catattcgcc agtgtgacat acgatgcggc ttcataacta
    13921 atcaccatat gggaaaatcc gttgcccgaa taatcatcgc tgcgtatggc cgacggtacg
    13981 tcatcaagaa tttttacggc cgatagtttg tacgatgtgc ctttataaat atgcagttct
    14041 tttgtcgctt tgattacaat cgcgatatca atcttaccat ccttgtcata atcgccaaga
    14101 ttgatcagcg aaggttcccc tttgattttg atcgggtctt tagctttaaa tccgccggat
    14161 ttttggttca taaaaacaga taccgtttgc gcagggccgt tggccgtaac gatatcaggc
    14221 ataccgtcat tatccaaatc gcccacctgt atgtctacgg caaacttctc gctggcaaga
    14281 tcaattttat ttttgaaagt tccgtcgcct ttgccgtaca aaacggaaat aatacccgaa
    14341 ccgtctaggt cgttttttcc ggaggtcacc acgtccataa atccgtcgcg attaaaatcc
    14401 ccgacatgga tggcaaatgg cgtaaaatag agattaaagg gtgtgcccgt cgtataaata
    14461 tcctgtccca atgtgggaag cgcgaaacac aatgtcgcaa tgacggtaag atatgaacgg
    14521 cgatgaaacg atttcataaa aaatctaaac atactaaatg atattgccct actcagaaag
    14581 gaagaaccaa attaaatggg aagaattttc ctcatgaagt gaatatgtcc aataagagct
    14641 attaaatcaa tagttattag gtagtttgcg ttttgtagta tatccgattt tgccggctct
    14701 ttttcggatt tagttatgct aatttagtct ttaagtacga cgtaagttcg ttgagtgaaa
    14761 tttgttcctg tgcaccatcg ctcatatttt ttacgacggc catgttttga gcgatttcat
    14821 tatcgccgag tattacggta aatcgtgcct gcatacgatt ggcatcgcgc atttgcgctt
    14881 tcatgcttcg cgccaataaa tccgtgtcac aggtcaaccc ttcgcgtctt gctttttgta
    14941 tcagcggcat agtggcccct accgcggccg cgccaagagc gataaaatac acatccaatt
    15001 tttcaggttc gccgatcgga ataccctccg cttctaaaac gatcatcaaa cgctcgatac
    15061 ctgctgcaaa acctacagcc ggtgtgggtt taccgccaaa ttcttccgcc aaaagatcgt
    15121 accgtccgcc gccgcccaac gcgttctgcg aacccagatt accgctttga atttcatatg
    15181 ctgttttggt gtaataatca agaccacgta cgagccgtcc gtctatttcg tacacgatgc
    15241 ccaacccatt cagcgaactt tggacgcgcg aaaaatgcgt tgcacagtcc gtacataaat
    15301 gatctttcat caacggcgcc gattgcgtca gcgcgatgtc gcgttcgtct ttggaatcca
    15361 aaatacgcat cgggttcaca accaagcgct tgcggctctc ttccgacaat tgccctttga
    15421 caccgtcaag aaatgccacc aaggcatcgc ggtaagcggg acggcattga ggacaaccga
    15481 cactattgat tttaaaattg agcgctttga tacccagctc ttcatataca gtaagtacga
    15541 ccaacatcgt ttcgacgtcg gcttccggtg aagcgctgcc gagcgcttcg actccgaact
    15601 gatgaaactg acgcagacgc cctttttgag gacgctcttg acgaaacatc gggcccatat
    15661 accagcattt attcaaagga agttgctcgc cgagactgtt ttcgatgtat gagcgcataa
    15721 ccgaagccgt caattcgggt cgcaacgtca gactctcctg gcctttatct aaaaaagtgt
    15781 acatttcttt tccgacaaca tccgtcgctt cgccgatccc gcgcgcaaaa agttcggtat
    15841 actcaaacat cggcgtgcga atttctttgt agttgaaacg gcgcatcaca tcggcgattt
    15901 tacgctcgac atattgccat ttataactgt cagacggtaa cacatctttg gttccggtcg
    15961 gcatacgata cgtcacaagg tactcctaaa agacttaagc ggtggattgc ggccgctgcg
    16021 atgtacgcgt gttttgagac aacatacgta attcttcggc ggacgataac atgtttttat
    16081 tttgtcgtaa tgattctttg gcgccggcat caatatcttt gatgctcatc cagagctgct
    16141 ccaaggcgct ctgctgctgc tggatcgctt gcgcgatctg ctggacggac tccatcgtct
    16201 ggcgcaccat ccagtatatt tgttcaaacg attcgcgcgc atccccggcg tgccggattc
    16261 cttctttgac ctgcatcagc tctttttcgc tggccagtac cgatacttgc gacgctttac
    16321 gcacgtcgag caggattttt ttgatctctt cggtggagtc ggcgatattt tgcgcgagat
    16381 gccggatctc tttcgctacg acgccaaatc gttcgcccat ttctccggcg cctgcggttt
    16441 cgatagccgc gttgaaagcc aatattttgg tttgatccgt gatgtaatta ataattttga
    16501 tcacatcttc gatttgcgtc attttgagac tcaaatctaa aatacgttgt gcgctggttt
    16561 gcgattcgcg atgtatctgc tccatactct gcaatgtatc cgctacatga cgtttcccgt
    16621 cttccgcttc ctgactgccc ttgagcgtgg attgattgac ggatttggaa ttttcggata
    16681 tctgcgcaac cgatttcatc agctcttcga gcgacgccgt gatctgcgcc aacgccgagg
    16741 catgattgga agcgtagctg ttttgcgatt ttaagccgga caaaatgtgt tcagcggtcg
    16801 caccaagggg ttggatttga tcacgtaaaa cgagcacctc ccgtttggcc gtggccgcaa
    16861 atcgttccgc cgttttttgt aatcggtata tatcacccgc atgcaacatg gtatgaccaa
    16921 cctcttcagt gtgatatatg tccggaccgg accagagtgt caaccaccga tcatgcgccg
    16981 tctgcaccgt acgggtcgta agccatccga taaagccaaa catgacaagc gccaaaagcg
    17041 taatcgcaac cggatgattg tgataattat acagcaaaag accgtaccac agcgtcatcg
    17101 cgaggatcgg ggccatgacg cgcgtacgca gacgaatcca gacgatatcc ggatcgttgg
    17161 cgtcaggcat ttgcgccaaa acttcgggaa accgcgaact gcatacatga attcgccact
    17221 ccagcacata aaataaaata atggaaaagg gaatcagacc aagcaatacc caaatcaacg
    17281 ttttatacat gtattcccat ccggaaatcg actcgattac aaacggcacg agcaaaaccg
    17341 atgaaagcaa taaactcatt cccgcgaggt gcacaaagta gagataaaaa accatcgttc
    17401 ggggatattt cagccattcg acccatatcc tgcgaataga ttcttcggat agcgtaccgc
    17461 tatggctttc ggcggtcaat gtttttcgaa aaagcgccag atgcatcatg ccgccgacta
    17521 cgatcgtaag cccgacaaca agaaaccccg tcacagaaac gaaaaacgat tcgtccggtg
    17581 aaagatcaaa caaccgctga tatgtgatca cattccaaat cgccatcacc agtgtcacgc
    17641 acagcgtggc taaaaaaggt gaccacagca tgacccggtt atccgatctg gaattgaata
    17701 aagcgctcgt ttcgtttaac atattatccg atcaggcttt cgactgtgtc aataagattg
    17761 gtttggtcaa atgatccttt gacgatataa gcattggcac ccgcttcgat accgcgacgc
    17821 ttatcctcgg cggaatcgcg cgatgtcaca atcaccacgg gtatgtgctg atacgaagct
    17881 tccgatttga tgcgcgaagt cagcgtgaga ccgtccatcg ccggcatttc caaatcggtt
    17941 accacaaggt cgtatttctt ttgctgtatt ttttccaaag catccaaccc gtctttggcc
    18001 gtttccacat cgtaaccata ggcctgcaat atggtttttt cgatttcgcg cgtgttgagc
    18061 gaatcgtcca cgaccaaaat actgcgcgct gatttgggaa tactttgcac ttgttcgcgg
    18121 actgaaaaat ctttggtcgc acggatgaga tcgggcgcgt gcaagaccat gatgat
//
LOCUS       DAOQGF010000067        18075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1446_length_18075_cov_5.723474, whole genome shotgun sequence.
ACCESSION   DAOQGF010000067 DAOQGF010000000
VERSION     DAOQGF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_18075_cov_5.723474"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PK937_10045"
     CDS             <1..730
                     /locus_tag="PK937_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32954.1"
                     /translation="LDSPGYDSWARVSAHIAVRGANSRKFSDTLRHSTADSREPFDAH
                     QFACLYLKLRMKRVIWTLLIAFLASSINAQEIFLRISKDIDGKHISESTNFWDLKEPL
                     TEKVHSIQFSGEMLFPVNKYFFIGGGMSKSTTMATKRWGGKFSVRPFYGALQIKLSNH
                     MNHVYFGFQYGVSVLSNNVSYEGGGTIKDGSYFGISTGLNFFFLNFDIGYQRHKARWK
                     FEDEKMNIKYNHVALGIGLRLKLK"
     gene            1068..1652
                     /locus_tag="PK937_10050"
     CDS             1068..1652
                     /locus_tag="PK937_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32955.1"
                     /translation="MSNKISIGLIQIALLLIFTDTIYCQTQALNDKRSAFGFGELGIG
                     FSEFGSAYYGGVTFFAGHNALKILVQRNKEIEGWIEWTERLPKEKLNQFCLMYGLRHS
                     SRRVTVAGFVGVSKLMGQIRGKHLYNQSEDENGDDWFAWFPDYAVYEKVKIKNYALSA
                     EGQIIFSFVGIKLFGTFAQKVSYVGAGLSLTLGH"
     gene            1787..2521
                     /locus_tag="PK937_10055"
     CDS             1787..2521
                     /locus_tag="PK937_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32956.1"
                     /translation="MSKNIKCCVIIFSLLQIGCGGISIIEHQDAETLNRGKFSVLTSA
                     SGGPEEWSKKPRDRFVKAIAPAILEPRLSYGISDRLNVGASLWSGYAAPVLLSKVVLG
                     ANIKSCDFGLRGMLKYKVTPDNSKMQMSLLLNYTNYFLFYSERTDKLASGISPGLIIS
                     KRSSQTSVMYGIIKYNIFNIDHDYGEGGKGSNPNNFPSFHIGYSENINKNKGFAELVY
                     SYVNNPLSIGHTGHVFSISVGYMWKL"
     gene            complement(2599..3567)
                     /locus_tag="PK937_10060"
     CDS             complement(2599..3567)
                     /locus_tag="PK937_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HNH32957.1"
                     /translation="MRFISSDTLRELITMPEAIVAMQSAFSALSDGSAVVPLRTTLDF
                     PGSDARALIMPAYHSSSQYFTTKIVTVCPTNAARHLPLIHGTLIVTDGSTGKTLALMD
                     AEWITALRTGAASGLATEWMALPDAHTLGVFGTGAQAYEQVRGVLSVRKLTRIIIFGR
                     YPQKTTTFCKTISNQFAIPCFPAQTPQEVSQCDILCCATNAHNPVFEDKYLTHGTHIN
                     GIGAFKPAMREIPSATIARARIIVDQRASAWREAGDLILAQKEGVVSEDCIAGEIGEV
                     VTHKASGRISREQITIFKSVGNAIQDNVIASMAYERTLKAGVGTEI"
     gene            complement(3573..4640)
                     /locus_tag="PK937_10065"
     CDS             complement(3573..4640)
                     /locus_tag="PK937_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNH32958.1"
                     /translation="MPEKKSEFEKRLRTMQRWEAPSSWRCIETIEAHTGGEPLRIIIS
                     GLPPIKGQTILQKRAYLKTHLDHLRTALMWEPRGHADMYGCIITPPVAKNSDFGVIFL
                     HNEGYSTMCGHGIIAVTKAALETGLIRANGTEVTLRIDSPAGLITSIAQIRRGKVQSV
                     RFLNVPSFVQTLDAVVDVPGLGRIRYDLAYGGAFYAFVHAEDASVTMDAAGYRGLIER
                     GMMIKRAVMQSVAMQHPFEPELNFLYGTIFTGPGHTTGADSRNVCIFADGEVDRSPTG
                     TGVSARMAIHHARGALRIGEPMAIESITGSRFVCRVHATTTYGNYPAVIPAIEGTAHL
                     CGKSSWIIDPADSLRDGFFFR"
     gene            complement(4633..5892)
                     /locus_tag="PK937_10070"
     CDS             complement(4633..5892)
                     /locus_tag="PK937_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH32959.1"
                     /translation="MSSKKTDVAIIGGGVIGLCTAYYLNKAGISVTIIEKKQPGFGSS
                     FGNAGLISPSHFVPLAAPGMVAKGLKWMFDAESPFYIHPRPDIDLITWLWKFMRASTD
                     KRMRPAMPVLRDMTLASQQLFHTIANEERFDFALEQKGLILLHDTAEGEKAHHHEVAL
                     ANEVGVTAHMISREEIHQMQPGVKVRATGGVFFPQDGHLIPDQFNAKLTSLLQTRGVQ
                     FMNETDVFGFVPNGKAAGHLRTSEGDIHADQYVVAAGAWTSDLLYEIGIALPVQAGKG
                     YSVTVKREKNNLTIPIICTEARVAITPMGDTIRYAGTLELSGNDLTINPRRVKAILRA
                     ADRYLENFDAQSSDIQTAWAGLRPCSPDGLPFIGRFKRAPNVIAATGHAMLGITLGPI
                     TGKLVSEIVTNTPQTITSPLLDADRYA"
     gene            complement(5938..6939)
                     /locus_tag="PK937_10075"
     CDS             complement(5938..6939)
                     /locus_tag="PK937_10075"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HNH32960.1"
                     /translation="MSRHTFSCIDAHTCGNPVRVVTSGGPVLLGANMSERRQHFLREF
                     DWIRKALMFEPRGHDMMSGSIFYPPIDPANDVAILFIETSGCLPMCGHGTIGTVTVLI
                     EQRLITPRTPGILRLEVPAGIVEAHYTIQHNKVTSVRIVNIPSFLHSQNLTVECPDLG
                     EITLDVAYGGNFYAIVDPQKNFKGLETISAGDVLRWSPIVRQRINEKYTFTHPENSTI
                     CGCSHLQWTGVPTQNEAHARNAVFYGDKAIDRSPCGTGTSARMAQWAAKGKLKTGDTF
                     VHESIIGSMFRGRVERTTHVGDYDAIVPSIEGWAYITGYNTIFIDTDDPYAYGFQVM"
     assembly_gap    6949..7048
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7110..7748)
                     /locus_tag="PK937_10080"
     CDS             complement(7110..7748)
                     /locus_tag="PK937_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNH32961.1"
                     /translation="MKVSLTDQAYDKIEEMLVTLKFKPGTIISEPEIIERIGMGRTPV
                     REALLRLGKEHMIKAIPRKGIYINEIHITDHIALMETRRALERIIAMRSANRATAEQK
                     KELTRLSQRIVDAAKKNDIETFIHADHAFDLIISEACRNPYASEACASLRTHCRRFWY
                     RFHHDQDLVTSAKLHAEVMSAIVAGNEERAGTASDKLMDYLDQMTRVAMNKG"
     gene            complement(7751..9196)
                     /locus_tag="PK937_10085"
     CDS             complement(7751..9196)
                     /locus_tag="PK937_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH32962.1"
                     /translation="MTTDIKRLYINGSWIDGGDTFANINPSDTSDLIAHYAVATADHV
                     HDAVASAKQAFPAWSLSTPQQRFNILDAVGAEILARQDELGRLLSREEGKTLPEGIGE
                     VVRAAMIFKFFAGEALRAGGERMASVRPGVMVDMMREPLGVVGLITPWNFPIAIPAWK
                     IAPALAFGNCVVFKPAELVPGCAWALTEILHKAGIPAGVFNLLMGKGSVIGRTIVEHP
                     YVNAISFTGSVGVGRQVAAQCAARLAKVQLEMGGKNPLVILDDADLSVAVNAAVQGAY
                     YSTGQRCTASSRLIVTEGIHDTFVKALTDKLQTLRIDDALAQGTDIGPVVDLTQMNKD
                     LEYITIGAQENAHLAFGGVALERSKKGYYLQPALFTETTSAMRINREEIFGPVASVIR
                     AKNYDEALAMANDTPFGLTSGICTTSLKFAEHFKRHAQAGMVMINLPTAGVDYHVPFG
                     GRKGSSYGAREQGTYAREFYTTVKTAYTFAG"
     gene            complement(9294..10190)
                     /locus_tag="PK937_10090"
     CDS             complement(9294..10190)
                     /locus_tag="PK937_10090"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNH32963.1"
                     /translation="MHSTWQGIFPATTTKFKPDFSVDYAAIEKHTTWMLEAGIHGLVV
                     LGSLGENLTLTEDEKLAILKTACNVCAGKIPVIATVMETSTAKACAFAEKAARAGAQG
                     LMVLPGVPYPSDARETITHYRTIAKATDIPIMIYNNPVAYKVDITPEMFAEMADEPHF
                     VAIKESSDNIRRVTDIYNRVGNRYRIFCGVDDLAMEALTLGADGWLAGLVVAFPHETV
                     AIYNLVREGKMKQALEIYRWFMPLLHLDVNTKFVQNIKLTEQLVGVGSEIVRAPRLPL
                     IGDERAHVEHIVREALVKRPKL"
     gene            complement(10319..11326)
                     /locus_tag="PK937_10095"
     CDS             complement(10319..11326)
                     /locus_tag="PK937_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32964.1"
                     /translation="MFSTIFIIIAGTVITIAGITAYNKIKGLILRKPKGFHLNLVEFF
                     LVPVVALLIVFEIWSRIVGTQEVHALEMSGNTLWALEHVSRGTGNWGERFSLRNVFGI
                     RVSDGTYLYKKSMEGNFQRIAQKENIAWCWDDETIVGVESSTGDVKYTIDPDRFESLP
                     AFRDGVAGFDMKADGIIRATNKSGQTINIDPLHFTEIAAPPDTIASEPITASSDSFKF
                     AMSDTTNAQLVNAAGQIISKDILTHGRILQVDPVQKIIITRHYENNDPQDFIISAYLF
                     NGTLRWQVRYDQLGVRRFFLKKSLLAHSLIADERFIFSAGNFVVALDIATGRLLWKTA
                     L"
     gene            complement(11337..11681)
                     /locus_tag="PK937_10100"
     CDS             complement(11337..11681)
                     /locus_tag="PK937_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32965.1"
                     /translation="MREQTHPIQSIEEELAEAELSLHSGNDGRARVQSRRAVGLAVTA
                     WHASRGEFSRRPAIEYLRHIAQDAALPTPVQKAAARLIKNVNDRLSADYTLHPVRDAR
                     IIITYFLQRLTP"
     gene            complement(11741..12634)
                     /locus_tag="PK937_10105"
     CDS             complement(11741..12634)
                     /locus_tag="PK937_10105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH32966.1"
                     /translation="MLAELATEIIPRSNTLSIQRVLPHVLCPECRSASFKMYADGSTE
                     LSVLTHGVIVCQSCHHCIRFEDGILEVLKEKPDHLSPAQRSNFFLPVAAGYQKCWRTW
                     CMHMMCGTSFPNEKEAALLISSLQLSSLPSSGIILDLGTSHGFYAVAIAQALERMQSD
                     ALVLAIDISKLMLQRAVQRAEEAGVLHRIIFILGDAEALPLADSTVCRVVSGGSLNEF
                     TRPDVAMSEAARTTQTDALFFNMFLHRTSWTAPLLSMIATLSGLHFHTAGTYNALFEK
                     TGWRIDLAETHGIVTFARLKK"
     gene            12794..13720
                     /locus_tag="PK937_10110"
     CDS             12794..13720
                     /locus_tag="PK937_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNH32967.1"
                     /translation="MGFEMMCLAFAGFILFLIIMSAFFVVKQQTVAVIERFGKFTRMA
                     ESGLRFKIPMVDRIAARVNMRVQQLDVVVETKTQDDVFVHLKVSVQFFVIPEKVYEAY
                     YKLSNVHQQVTSFVFDLVRARVPKLKLDDLFEKKDEIADAVRHELSDTMEDFGYAVLK
                     ALVTDIDPDAKVKEAMNEINAATRMRMAAAEKGEADRILKVKAAEAEAQSKALQGKGI
                     ADQRKAIIEGLRDSVEQFQQAVEGSSAKDVMMLVLMTQYFDTLQNLGEHSHTNTILMP
                     HSPGGLNAIADQIRDAMITAGEANKKQPAKPQ"
     gene            13844..14392
                     /locus_tag="PK937_10115"
     CDS             13844..14392
                     /locus_tag="PK937_10115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNH32968.1"
                     /translation="MHIVLFLGSLRKDSFNRKLLNNVLPMLEGHTTDVVDLKETVLPV
                     FDGDLDGDKTPPETQRLAERVKRADAVIFVSPEYNYNIPGGLKNAVDWVSRSKERPLH
                     DKPVMLTGATQGPFGCVRLFIAMRQMLVFFNAFVIPRQVGIPSAAKAFDEQGKLIDPK
                     QVEMLQTAVHELLHVTQALSKK"
     gene            complement(14460..15260)
                     /gene="egtC"
                     /locus_tag="PK937_10120"
     CDS             complement(14460..15260)
                     /gene="egtC"
                     /locus_tag="PK937_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HNH32969.1"
                     /translation="MCRLVLYKGPAISLGHLLHTPKHSIIKQSYEAREMLRGTTNEDG
                     FGLAWYAPHWSDEPAVYRSIQPISNDVNMHTMAGKISSDIILAHVRGASEGMPVAITN
                     THPFGWQRYAFMHNGAVDEFRSRFFPELFNDIHPSLWSHIKGNTDSEHVFGLWLSYLG
                     LDKNASLHEQVSALRRTFQRLETIAQKQNLDIVLNIGISDGQNIIAARFHYGSRKATL
                     YYNDNLALFPDAVMIASEAQTDDAGWKPVPERSILTITSNNTLRVETV"
     gene            15407..16222
                     /gene="speB"
                     /locus_tag="PK937_10125"
     CDS             15407..16222
                     /gene="speB"
                     /locus_tag="PK937_10125"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNH32970.1"
                     /translation="MNAFPVFPSAGRIGLLGVAFDQNSSYQRGAAGAPQRIRKAFFCD
                     SSNLWSENEMDLGAEGIFTDMGDISPEPSQLLAETERAAGQLYESGLKVIAFGGDHSV
                     TFPLVKAAAKTHRNLSILHFDAHPDLYDDFEGNPHSHASPFARIMESGLAKRLVQVGI
                     RTINGHQRAQAQRFGVEVLTMQRWRDIFDVRWDSPLYISFDMDCLDPAFAPGISHREP
                     GGLSTREVLSIIQSLNADIIGADIVEYNPEMDINSTTDMVAAKVFKEIAAIML"
     gene            16345..>18075
                     /locus_tag="PK937_10130"
     CDS             16345..>18075
                     /locus_tag="PK937_10130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNH32971.1"
                     /translation="MNRKHVCFVGMLMILMPGFLFTQKKTEVPSGMEPGLYKSMEWRQ
                     IGPFRGGRSVAVAGDVRDPLTFYFGATGGGVWKTTDGGITWLNVSDGFFKTSAVGALS
                     VAESDPNVIYAGMGETCIRGNITLGDGIYKSLDAGKTWSHTGLKETQFIGGIAIHPQN
                     PDFVLVAAMGHIFGTNKERGIYKTTDGGKTWKQTLYKDDKTGAVEVALDPRNPRIVYA
                     ALWEAHRNAWSMTSGGSGSGLYKSTDGGETWTDISKYPGLPKGVIGKIGISVSPVKDG
                     LVWACIENDNGGVFRSDDGGQTWRRTNEERNLRQRAWYYSHIYADPVNSESVYALNVQ
                     FFKSVDGGRTFKNFETKHGDHHDLWIDPRNSQRMIIADDGGAAVSYNGGVSWTDLDIP
                     TAQFYHVSVDNQFPYNIYGAQQDNSTVRISSRTTSYGIDFADWSIVAGGESGYVVAHP
                     SKPTVTFGGSYGGYLTRLDATTKQERNIAVWPDNPIGAGAADQKYRFQWTYPIVFSPH
                     DKNTLYVTAQCVFRSTDEGQSWTVISGDLTRNDQSKQLSSGGPITKDNTSVEYYNTIF
                     SLAESYKEKGV"
BASE COUNT         4713 a         4007 c         4502 g         4753 t
ORIGIN      
        1 attagattct cccggatacg atagctgggc acgcgtctcg gcacatatcg cggtgcgagg
       61 ggctaatagt cgaaaatttt cagataccct gcgccacagc accgcagata gtcgcgaacc
      121 gtttgacgca catcaatttg catgtttata tttaaaacta agaatgaaaa gagtaatctg
      181 gacactttta atcgcatttt tagcatcttc aatcaatgct caagaaatct ttttaagaat
      241 ctcaaaagat attgatggaa aacatatatc agagtcaaca aacttttggg atttaaaaga
      301 gccattaact gagaaagtac attcaataca attttcgggt gaaatgctct ttccggtaaa
      361 caaatatttt tttataggtg gtggtatgag caaaagtacc acaatggcga ctaaaagatg
      421 gggagggaaa tttagcgtaa gaccatttta tggtgccctg caaattaagt tatcgaacca
      481 catgaaccac gtgtattttg gctttcaata tggggtttca gtactatcta ataatgtttc
      541 ttatgaaggg ggaggaacga tcaaagatgg ttcatatttt gggatttcta cgggtttaaa
      601 cttctttttc ttaaattttg atattggtta tcaacggcat aaagcgagat ggaagtttga
      661 ggatgagaag atgaacataa aatataacca tgttgcacta ggaattggtt tgagattgaa
      721 attgaaataa attgatgagc gtctaacctt ccggtgatct gtacgtatgc tgatcataga
      781 gtattgagaa tcggcttttt atagatgaat gtaacaataa attagtcacg cagatcgtat
      841 atatcgcgcc gcgatgaagg agtaagtgct agttttcaaa tactccgtcc cacgacacat
      901 tgtctataga tcgcctttaa gcgcgattgc gtttctgcaa taaatttctt gcagtgtgaa
      961 aaataattaa agatttatta caatcgcctt tttgatttta ataatgttag ctttggtata
     1021 gatgttgccc cttgctgtgt ttataaaact tcacggggga actaatcatg tcgaacaaaa
     1081 tttcaattgg tttaattcaa atcgctctgt tactcatatt tactgatact atttattgtc
     1141 aaacgcaggc tctaaatgac aagcgttcag catttgggtt tggagagtta ggaatcggat
     1201 tttctgaatt tgggtcagcg tattatggag gagtgacttt ttttgcgggg cataacgcac
     1261 tcaaaattct tgttcagcgt aacaaagaaa ttgaaggttg gattgagtgg accgaaagat
     1321 tacccaaaga aaagctcaat caattttgct taatgtatgg attgaggcat tcatctaggc
     1381 gagtgaccgt cgcaggattt gttggggtta gtaagttaat gggccaaata agaggtaagc
     1441 atctttataa tcaaagtgag gatgaaaatg gagatgattg gttcgcttgg tttccagatt
     1501 atgctgttta tgaaaaagtg aagataaaaa actatgcgct ttcagcagag ggtcaaataa
     1561 tattctcgtt tgtcgggatt aagttgtttg gtacttttgc tcaaaaagta agttatgtgg
     1621 gtgctggatt gagtttgacg ctagggcatt gactcgttcg cacttcacat acatcgtggc
     1681 gcgagacacg agtggtttga aattttcgaa accttatacc gcaccatcgc agatagtcgc
     1741 gaaccgttat gtaccatgcg gttgtattct ataaaaacgg gcaacgatga gtaaaaatat
     1801 taaatgctgt gtaattattt tttccttact tcaaattggt tgtggcggta taagtataat
     1861 tgaacatcaa gatgcggaaa cattaaatcg tgggaaattc agtgtactga ctagcgcttc
     1921 aggtggtcca gaagaatggt caaaaaaacc aagagatcgt tttgtaaaag cgattgcacc
     1981 cgcaatactt gaacctaggt tatcgtatgg tataagtgac cgcctaaatg taggggcaag
     2041 tttgtggagt gggtatgccg caccggtgtt actctctaaa gttgtgttgg gtgcgaatat
     2101 aaaaagttgt gattttggct taagaggaat gttaaaatat aaagttactc ctgataattc
     2161 gaaaatgcaa atgtctcttt tgctgaacta tactaattat tttttatttt attctgaacg
     2221 aacggataaa cttgcctcgg gtatttcccc gggattaatt atctcgaagc gcagctcaca
     2281 aacaagtgtt atgtatggaa taattaaata taacatattt aatatagatc atgattatgg
     2341 tgaaggtggg aaaggttcta atcctaataa cttcccttct tttcatatag gttatagcga
     2401 aaatataaat aagaataaag gtttcgccga attggtctat tcgtacgtga ataacccatt
     2461 aagtataggt catacaggtc atgtttttag catcagcgtt ggttacatgt ggaaattatg
     2521 aagtcatggt ctacggcatg tcaaaacaaa tatatatttc gaatatgaac aagttgtttt
     2581 ctaatactaa tcgtaggttc aaatctccgt tcctactccc gctttgaggg tacgttcata
     2641 cgccatggat gcaattacat tatcctgaat cgcattacct acggacttga agatggtgat
     2701 ttgttctcgg gatatacgtc cggaagcttt atgcgtgaca acttcgccga tttcaccggc
     2761 aatgcaatct tcagagacta ctccttcttt ttgagcgagg atcaagtcgc cggcttcacg
     2821 ccatgccgat gcgcgttgat ctacgataat gcgagcgcgt gctatagtcg ctgatggtat
     2881 ttcacgcatt gcgggtttga atgcgccgat accattgatg tgcgtaccgt gcgtgagata
     2941 cttatcttca aaaaccgggt tatgcgcatt ggttgcgcag cagagaatat cacattggga
     3001 aacctcttgc ggcgtttgag cgggaaaaca aggaattgca aattgattcg aaatagtttt
     3061 acaaaacgta gttgtttttt ggggatagcg gccaaatata attatccgtg ttaattttct
     3121 tacactcaag acgccacgca cctgttcgta ggcctgcgca cctgtcccga aaactcctaa
     3181 tgtatgcgca tcgggaagag ccatccattc cgtggccagt ccggaagccg caccagtccg
     3241 caaagccgta atccactctg catccatcag tgccagtgtt tttcctgtgg atccgtccgt
     3301 aacgatcagt gtaccgtgga tgagcggaag atggcgcgcg gcattggtcg gacataccgt
     3361 cactattttg gtcgtgaaat actgagatga tgaatgataa gccggcatga tcagcgcacg
     3421 ggcgtcggaa ccgggaaagt caagggtcgt tcgaagcggc acaacagccg acccatcgga
     3481 taatgcagaa aatgccgatt gcattgccac aatcgcttca ggcatagtga tcaactcacg
     3541 cagtgtgtcg cttgaaataa atcgcatgga ttttagcgga agaaaaaacc gtcccgcagc
     3601 gaatcggcag gatctataat ccaggagctt tttccgcaga gatgcgccgt gccttcgata
     3661 gcggggatca ccgccgggta gttaccatac gtcgtcgtcg catgaacgcg gcatacaaag
     3721 cgactgcctg taatgctttc gatggccatc ggttcaccga tgcgaagcgc accgcgtgca
     3781 tggtgaatag ccattcgcgc gctgactccc gttcctgtcg ggcttcgatc cacttcgccg
     3841 tcggcaaaaa tacagacgtt gcgggaatct gcaccggtcg tatgtcccgg gccggtgaaa
     3901 atcgtaccgt ataaaaaatt aagctccggt tcgaaggggt gttgcatggc tacactctgc
     3961 ataactgcgc gtttgatcat catgccgcgt tcgataaggc cgcgatatcc tgccgcatcc
     4021 atcgttacac tggcatcttc ggcgtgcaca aaagcgtaaa atgcaccgcc gtacgcaaga
     4081 tcataacgta tgcgtcccaa tcccggtacg tcgacgacgg catccaaagt ttgtacaaac
     4141 gatggaacat tgagaaaacg aacggattgt actttgccgc gccggatttg ggctatggaa
     4201 gtgatcagtc ctgcgggtga atcaatgcgc agtgtgactt ccgtaccgtt tgcacgaata
     4261 agcccggttt ctagggccgc tttggtaact gcgatgatgc cgtgaccgca catggtcgaa
     4321 taaccttcgt tatgcaaaaa aatcacaccg aaatcggaat tttttgccac cggcggagtg
     4381 atgatacagc cgtacatatc ggcatgcccg cgcggttccc acatcagcgc cgtacggaga
     4441 tgatcaagat gcgtcttgag gtacgcccgt ttctgaagaa tggtttggcc tttgatcggc
     4501 ggtaatcctg atatgatgat gcggagcggt tcaccgccgg tatgagcttc gatcgtttcg
     4561 atacatcgcc acgatgaagg cgcttcccat cgctgcatcg ttcgcaggcg cttttcaaat
     4621 tccgattttt tttcaggcat agcggtcagc atccaataat ggcgaagtaa tggtctgtgg
     4681 cgtattggta acaatttcgg agaccagttt ccccgtaatc ggtccaaggg taatacccag
     4741 catcgcatga ccggtcgcag cgattacgtt gggggcgcgt ttaaatcggc cgatgaaagg
     4801 taatccgtcc ggtgaacacg ggcgaagacc ggcccacgcc gtttgtatat ctgacgattg
     4861 cgcatcaaaa ttttcaagat agcggtctgc ggcccgaagt atcgccttca cacgacgcgg
     4921 attgatggtc aggtcgttgc cgctcaactc cagcgtaccg gcgtaacgaa tcgtatcgcc
     4981 cattggagtg atggctacac gcgcttcggt acaaatgatc ggaatcgtaa gattattctt
     5041 ttcgcgttta accgtgacgc tataaccctt gccggcttgc accgggagcg caataccgat
     5101 ttcgtagagc agatcagacg tccacgcgcc ggccgcgacg acatactgat ctgcatggat
     5161 gtcgccctcg ctggtacgca gatgtcccgc cgctttgccg ttggggacaa agccaaatac
     5221 atcggtttcg ttcataaact gcacaccgcg tgtttgcaat aacgacgtga gcttggcgtt
     5281 aaactgatcg gggatgagat gcccgtcttg gggaaaaaat acgccgcccg tggcgcgcac
     5341 ttttacgccg ggttgcattt ggtgaatctc ctcccgcgag atcatatgtg ccgtaacgcc
     5401 gacttcattg gctaaagcta cttcgtgatg atgcgctttt tcaccttcgg cggtatcatg
     5461 caaaagaatg aggccttttt gttccaaagc aaaatcaaaa cgctcttcat tggcaatggt
     5521 atgaaaaagc tgctgactgg ctaatgtcat atcgcgtaat accggcatag ccggacgcat
     5581 ccgcttatcg gtactcgcgc gcataaactt ccacagccat gtgataagat caatgtccgg
     5641 acgcggatga atataaaacg ggctttctgc atcaaacatc catttgaggc ctttggcaac
     5701 catacccggc gccgccagag gaacgaaatg actaggactg atgagaccgg cattgccgaa
     5761 agacgaacca aagccgggtt gttttttttc gatgatcgta accgagatgc cggctttatt
     5821 gaggtaatac gctgtacata atccgatcac acctccgccg atgatggcga catctgtttt
     5881 ttttgaactc atttgcgtat ccgaattaaa ttttttctat atgtttaccg tatcccgtta
     5941 cataacttga aaaccgtatg cgtagggatc atccgtatct ataaaaatcg tattgtaacc
     6001 tgtgatgtaa gcccatcctt cgatgctggg aacgatggca tcgtaatcgc cgacatgcgt
     6061 tgtgcgttcc acgcggccgc gaaacatact tccgatgata ctttcatgta caaacgtatc
     6121 gccggttttg agttttcctt tggccgccca ctgggccatg cgcgcggaag tgcctgtgcc
     6181 gcagggtgag cggtcaattg ctttatcacc gtagaatacg gcattgcggg cgtgggcttc
     6241 gttttgtgtc ggaacgcccg tccattgtaa atgggaacag ccgcaaatcg tggaattttc
     6301 gggatgggtg aaagtatatt tttcgttgat tcgctggcga acgatcggac tccatcgtaa
     6361 aacatcaccg gcagaaatcg tctcaagacc tttaaagttt ttttgcggat ccacgatcgc
     6421 ataaaaatta ccaccgtatg caacatccag tgtgatttcg ccaagatcgg ggcattcgac
     6481 ggtaagattt tgcgaatgga ggaacgaggg gatattgacg atgcgaacac tggtcacttt
     6541 attatgttgg attgtataat gtgcctcgac gataccggcg ggaacttcca agcggaggat
     6601 gccgggtgtg cgcggcgtga tcaaacgttg ctcgatgagc acagtgactg tgccgatcgt
     6661 gccatgacca cacatcggca ggcagccgct cgtttcgata aataatatcg caacatcatt
     6721 ggccggatca atcggaggat aaaaaatgct gcctgacatc atatcgtgac cgcgcggttc
     6781 gaacataagc gctttacgta tccagtcgaa ttcgcgaaga aagtgctgac ggcgttcact
     6841 catattagcg cctaagagta ccggaccgcc gctggtaaca acgcgtaccg gattgccgca
     6901 ggtatgtgca tcaatgcatg aaaatgtatg tcgtgacata atgacctann nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ttctaaattc taaattctaa attctaaatt
     7081 ctaaattcta aattctaaat tctaaattct tatcctttat tcatcgcaac gcgcgtcatc
     7141 tgatccagat aatccatcag tttgtcggaa gcggtacccg cgcgctcttc attacccgcg
     7201 acgatagcac tcataacctc agcgtgcagt ttagcggaag ttaccaaatc ctgatcatga
     7261 tgaaaacgat accagaagcg tcggcaatgc gtgcgaagcg aagcacaggc ttcggaggca
     7321 tacggattgc gacaggcttc actgatgata aggtcaaacg catggtcggc atgaatgaaa
     7381 gtttcgatat catttttttt cgcggcatcc acgatacgtt gcgagaggcg ggtcagttct
     7441 tttttctgtt ccgccgtagc gcgattggcc gaacgcatag caataatgcg ttccagtgct
     7501 cgacgcgttt ccatcaacgc gatgtgatcg gtgatgtgga tttcgttgat ataaatgcct
     7561 ttgcgcggaa tggctttgat catgtgttcc ttgcccaatc gcaataaagc ctcgcggacc
     7621 ggagtgcgcc ccatgccgat ccgttcgatg atttcaggtt cggatatgat cgttccgggt
     7681 ttgaatttta acgtgacgag catttcttcg attttatcgt aggcctgatc ggtgagactg
     7741 actttcatag ttaccccgca aaggtatatg ccgtttttac tgttgtataa aattcgcgtg
     7801 catacgtgcc ttgttcacgc gccccatagc tggagccttt gcgtcctccg aagggtacat
     7861 gataatccac tccggcggtg ggcagattga tcatcaccat gccggcctgc gcgtgccgct
     7921 tgaagtgttc tgcgaattta agcgaagtcg tgcagatacc cgacgtcaga ccgaacgggg
     7981 tatcgttggc catagctaag gcttcatcat aatttttagc gcgtatcact gatgcgacgg
     8041 ggccgaaaat ttcttcgcgg ttgattcgca tggcagaggt ggtctctgta aacagtgcgg
     8101 gttgcaggta ataacctttt ttgctgcgtt cgagtgcgac accgccaaac gcaagatgcg
     8161 cattctcttg tgcgccgata gtaatgtatt cgaggtcttt attcatctgt gtcagatcaa
     8221 cgaccgggcc gatatccgtg ccttgcgcca gtgcatcatc tatgcgcagg gtttgaagtt
     8281 tgtccgtcag agctttcaca aatgtatcat gaataccttc ggttacgata aggcggcttg
     8341 acgccgtgca tcgctggcct gtggaataat aggcgccttg tacggcggcg ttgacggcga
     8401 cggacagatc ggcatcatct aagattacga gaggattttt ccctcccatt tccagttgta
     8461 ctttggccaa acgtgccgca cactgtgccg caacttgtcg tccgacaccg acggagccgg
     8521 taaagctaat ggcatttaca tagggatgct cgacgatcgt acgaccgatg acggatcctt
     8581 tgcccatgag taagttaaac acaccggccg gaattccagc tttgtgtaaa atttcagtca
     8641 aagcccatgc acaaccgggg actaattcag ccggtttaaa aacgacgcag ttaccgaatg
     8701 ctaatgccgg ggctattttc cacgccggta ttgcgatcgg aaaattccat ggcgtgatga
     8761 gtccgacgac accgagcggt tcgcgcatca tatctaccat aacccccggt cgtacggagg
     8821 ccatgcgttc gccgccagca cgcagagctt ctccggcaaa aaatttgaat atcatcgcgg
     8881 cgcgtaccac ttcgccgatt ccttcgggca atgtttttcc ttcttcccgc gacagcaaac
     8941 ggcctagttc atcttgccgt gctaaaattt cagcaccaac ggcgtcaaga atattaaacc
     9001 gctgttgcgg agtggaaagc gaccatgcgg ggaatgcttg tttggcggat gcgacggcat
     9061 catgtacatg atcggccgtg gcgacggcat aatgagcgat caggtcggac gtatcggacg
     9121 gattgatatt ggcaaacgta tcgccgccgt caatccatga accgttgata tacaaacgtt
     9181 tgatatcggt ggtcatgtgt aatatttgtt tagatgagaa ctacagaatg tgtaaccaaa
     9241 gaagtgctga ccaatgtaca tccctgatcg gatatagtaa atcagatttt ttgtcagagt
     9301 tttggtcgct tgactaaagc ttcccggacg atatgttcga cgtgcgcacg ctcatctccg
     9361 atcaaaggca atcgtggtgc acgaacgatc tccgaaccga caccgaccaa ttgttccgtc
     9421 agcttgatgt tttggacaaa tttggtgttc acgtccagat gtagtagcgg catgaaccat
     9481 cggtagattt cgagcgcttg tttcatttta ccttcgcgca cgagattgta gattgctacc
     9541 gtttcatgcg ggaaagccac taccaacccg gccaaccatc cgtccgcgcc gagagtgagc
     9601 gcttccattg ccagatcatc cacgccgcaa aaaatccgat aacggtttcc tacgcgatta
     9661 taaatatccg tcacgcgtcg aatattatcg gacgattctt tgatcgctac aaaatggggt
     9721 tcgtccgcca tttccgcaaa catttccggt gtaatgtcta ctttataagc caccggatta
     9781 ttatagatca tgatcgggat gtcggttgcc ttggcgatcg tgcggtaatg tgtgatcgtt
     9841 tcacgggcat cggacggata cggaacgccg ggcaatacca tgagtccctg tgcaccggcg
     9901 cgcgcggctt tttctgcaaa agcacaagcc ttggccgtgg aagtttccat cacggttgca
     9961 atgaccggga tttttccggc gcaaacgttg catgccgttt tgagtatggc gagtttttcg
    10021 tcttcagtga gggtgaggtt ttctccaaga gaacccagaa caacgaggcc gtgaattccg
    10081 gcttccagca tccacgtcgt atgcttttcg atcgccgcat aatctacgct gaagtcgggt
    10141 ttaaatttag ttgtcgttgc aggaaatatt ccttgccatg tggaatgcat gttttatctc
    10201 catttgtaat atacgtgtaa tatatcacag aatatttgaa agacaataaa tttttaaaac
    10261 gcgattataa aataatcgat ccgatctgcg aacatagttt ctttaaagaa gggtattctt
    10321 ataacgccgt tttccataaa agtctgccgg ttgcaatatc caatgcgacg acaaagttac
    10381 cggctgaaaa tataaaccgt tcatcggcga tcaagctgtg cgccagtaag ctctttttga
    10441 ggaaaaaacg ccgaacaccg agttgatcat agcgtacctg ccagcgcaat gtcccgttaa
    10501 aaaggtacgc ggatatgata aaatcttgcg gatcgttatt ctcataatgc cgggtgatga
    10561 tgattttttg aacgggatcc acttgcaaaa tccgcccgtg cgttaaaatg tctttagaaa
    10621 taatttgacc ggccgcgttg accaattgcg cattcgtcgt atcggacata gcaaacttga
    10681 aagaatccga cgaagccgta atgggttcgg atgctattgt atccggcggt gcagcgattt
    10741 ccgtaaaatg gagcgggtca atgtttatcg tctgaccgga tttattagtg gcacgtatga
    10801 taccatcggc tttcatatca aaaccggcca ccccatcgcg aaacgccggt aacgattcaa
    10861 atcggtcggg atcaatcgtg tatttcacat caccggtcga agattcaaca ccgacgatcg
    10921 tttcatcatc ccagcaccag gcgatatttt ctttttgagc gatacgttga aaattgcctt
    10981 ccatggattt tttataaagg tacgtgccgt cgctaacgcg gataccgaat acattgcgca
    11041 gagaaaaacg ttcgccccaa ttaccggtgc cgcgtgagac gtgttcgagt gcccagagcg
    11101 tgttgccgct catttccaga gcgtgcactt cctgggtacc cacgatgcgc gaccatatct
    11161 cgaaaacgat cagaagcgcc acgaccggca cgaggaaaaa ctcaacgagg ttaagatgga
    11221 agcctttggg ttttctgaga atcaggcctt tgattttgtt atacgcggtg ataccggcaa
    11281 tggtgatgac agtacccgcg atgatgatga atatggttga aaacataggc gatggattaa
    11341 ggggttagac gttgtaaaaa ataagtgatg ataatgcgtg cgtcgcgtac cgggtgcaac
    11401 gtataatcag cggataagcg atcgttgacg tttttgatca gacgtgcggc ggctttttgt
    11461 acgggcgtcg gcagcgcggc gtcctgcgcg atatggcgca aatactcgat cgcaggacgt
    11521 cttgagaatt cgccgcggga ggcatgccat gccgtgacag ccaatcctac ggcccggcgt
    11581 gattgtaccc gcgcgcgtcc gtcattacca ctatggagcg ataattccgc ttccgccagt
    11641 tcttcttcga tactttggat aggatgggtt tgttctcgca taacacaaac taaaatacaa
    11701 tgatgtggtg ttcaataaaa accgaaatca aaaaacgatt ctattttttt agacgtgcaa
    11761 aagttacaat gccgtgcgtc tccgccaaat cgatgcgcca tccggttttt tcgaaaaggg
    11821 cgttgtacgt gccggccgta tgaaaatgta aaccggaaag tgtggcgatc atggataaaa
    11881 gcggcgccgt ccatgatgtg cgatgcagga acatattaaa aaaaagtgca tcggtttgtg
    11941 ttgttcgcgc ggcttcgctc atggcgacat cgggccgggt aaattcattg agcgaaccgc
    12001 cgcttactac gcgacaaacc gtcgaatccg ccaaaggtaa cgcttcggca tcacctaaaa
    12061 taaaaattat gcgatgtaaa acgccggcct cttcggctct ctgaacagcg cgttgcaaca
    12121 tgagtttgga aatatctatc gccagtacaa gggcatcact ttgcatgcgt tcgagcgctt
    12181 gagcgatcgc aaccgcgtag aaaccatggc tggtgccgag atccaggatt atgcccgacg
    12241 aaggtaacga cgaaagttgg agcgatgaga tcagaagcgc ggcttctttt tcgttgggga
    12301 acgacgtacc gcacatcatg tgcatacacc acgtacgcca gcatttttga taaccggcgg
    12361 caaccgggag aaaaaaatta ctacgttgtg cgggcgaaag atgatccggt ttttctttga
    12421 gcacttcgag gataccgtct tcaaaacgaa tacaatgatg gcaggattgg caaacgatca
    12481 cgccgtgcgt cagcaccgat aattccgtag atccgtcagc atacattttg aatgatgcgg
    12541 atcggcactc ggggcacaat acatgcggaa gtactcgctg tatagataaa gtattggagc
    12601 ggggaatgat ttcggtagcc agttcagcaa gcatggagcg atgaatgcgt tcagtatgga
    12661 tttatttcat tggcgcgaaa atacgcgaat tcattagcca atgtccattc ttttttttaa
    12721 aatgacacca ttgaggaatt taatcataca acaatatatc gcgtaacgta tccattaaaa
    12781 cggagattca aatatgggct ttgaaatgat gtgtttggcg tttgcagggt ttatcctgtt
    12841 tctgattatc atgagcgctt tttttgtggt caagcaacag accgttgccg tgatcgagcg
    12901 tttcggaaaa ttcacacgga tggccgagtc gggtttacgt tttaaaattc cgatggtgga
    12961 tcgtatcgcc gcacgggtca atatgcgcgt gcagcaactc gacgtcgttg tggaaaccaa
    13021 aacacaggat gacgtatttg tgcatctcaa agtatccgtt cagttttttg tgataccgga
    13081 aaaagtgtac gaagcctatt acaagttatc caatgtgcat cagcaagtaa cttcgttcgt
    13141 atttgatctt gtgcgtgcgc gcgtgcccaa gctcaaactg gatgatttgt ttgagaaaaa
    13201 agacgagatc gcggatgccg tgcgtcatga gcttagcgat acgatggaag atttcggata
    13261 tgccgtactc aaagcgctgg tcaccgacat tgatcccgat gccaaagtga aagaagcgat
    13321 gaatgaaatc aatgcggcaa cgcgtatgcg tatggccgcg gccgaaaaag gcgaagccga
    13381 tcgtattctc aaggtcaaag cggccgaggc cgaagcgcag agtaaagcac ttcaggggaa
    13441 aggtatcgcc gatcaacgta aagccatcat cgaaggattg cgcgattccg tcgaacaatt
    13501 tcagcaggcg gtcgaaggtt cttccgccaa agacgtaatg atgcttgttt tgatgacgca
    13561 gtattttgat acgttgcaaa atctgggtga acattcacat accaatacga ttctgatgcc
    13621 ccattccccg ggcggactca atgcgatagc tgatcagatt cgcgatgcga tgatcacggc
    13681 cggagaggca aataaaaaac agccggccaa accgcaataa tttctttgtt aaatcggacg
    13741 ggtcagtgtg cggaatattt ctgtaacgga cccgttttta tatcctgagc gaatcactcg
    13801 gagaatttcg ttaacaaaac ataaagacaa ggagcgcatc gttatgcata ttgtactttt
    13861 tctcggcagt ctccgcaaag attcgttcaa ccgaaaacta ttgaataatg ttttgccgat
    13921 gcttgaaggg cacacaacgg atgtggtaga tttgaaagaa accgtgcttc cggtatttga
    13981 cggtgatctg gacggtgata aaacgcctcc ggaaacgcaa cgccttgccg aacgtgtaaa
    14041 acgtgccgat gcggttattt ttgtttcacc ggaatataac tataacatac cgggcggttt
    14101 gaaaaacgct gtggattggg tttcgcgaag caaagagcgt ccgttgcatg ataaacccgt
    14161 catgctcacc ggtgcaacac aagggccgtt cgggtgcgta cgtttattta ttgctatgcg
    14221 gcagatgctc gtatttttta acgcgttcgt tattccgcgt caggtaggta ttccgtcggc
    14281 cgccaaagcg tttgatgaac aagggaaact catcgatcct aaacaggtcg aaatgctgca
    14341 aaccgcggtt catgaattat tacatgtgac acaggcatta agcaaaaaat agagggcttc
    14401 gaaaaatatc gcgcttaatc aacgtgcgtt tattacaggt ttgaattgga gataagtttt
    14461 taaactgttt cgacgcgaag ggtgttgttt gaagtaattg tgaggatact gcgttcgggt
    14521 acgggtttcc atcctgcatc gtcggtttgc gcctcggatg cgatcatcac agcgtcggga
    14581 aataacgcga ggttgtcgtt gtaatacaat gtagccttgc ggcttccgta atgaaaccgt
    14641 gcggcaatga tgttctgccc gtcggaaatt ccgatattca atacgatgtc taagttttgt
    14701 ttttgtgcga tggtttccaa tcgctgaaat gtgcgacgaa gcgccgatac ttgttcatgc
    14761 aacgacgcat tcttatctaa accaaggtaa cttaaccata atccgaaaac atgttcggaa
    14821 tcggtattgc ctttgatgtg cgaccaaagc gacggatgaa tatcgttgaa taattccgga
    14881 aaaaaccgtg agcgaaattc atccacggcg ccgttgtgca taaaagcata acgttgccaa
    14941 ccaaaaggat gtgtattggt gatcgcgacg ggcatacctt cggaagcgcc gcgcacatgc
    15001 gccaaaatga tgtccgaact tattttgccg gccatcgtat gcatgttgac atcattgctg
    15061 atcggttgga tactgcgata aacggcgggt tcgtcggacc aatgcggcgc ataccacgcc
    15121 agtccgaatc cgtcttcgtt ggttgttccg cgcagcatct cccgcgcttc ataactttgt
    15181 ttgatgatcg aatgcttcgg tgtgtgaagc aggtgaccta gcgatattgc cgggcctttg
    15241 tataaaacta atcggcacat gggtgtatga tacgctttga atggaaaaaa accaacgatg
    15301 aaaaataaaa cacacatcat tgtacgtgtt tagcttaacg cgtgcgtatg ggtgctccgt
    15361 acactaaaaa aattataaaa cattcctgtg aggtcacgag gccaatatga acgcttttcc
    15421 cgtatttccg tcagccggac gcatcggttt attaggcgta gcatttgatc aaaactcatc
    15481 ctatcaacgt ggcgcagcgg gcgctccgca gcggatccgc aaagcattct tttgcgattc
    15541 ctccaatctt tggagtgaaa atgaaatgga tcttggtgcc gaaggcattt ttacggatat
    15601 gggagatata tcgccggagc cgtcgcaatt gcttgcggag acggaacgtg cagccgggca
    15661 actttatgaa tccggtttga aggtcattgc ctttggcggc gatcattccg tgacttttcc
    15721 gctggtcaaa gcggcagcga aaacgcatcg taatctcagt attcttcact ttgatgcaca
    15781 tcctgatttg tatgatgact tcgagggcaa cccgcattcg cacgccagtc cctttgcacg
    15841 aatcatggag tcgggtttgg cgaaacgtct ggtgcaggtt ggaattcgca ccatcaatgg
    15901 acatcaacgt gcgcaagcac agcgttttgg tgtggaggtt cttacgatgc aacgctggcg
    15961 tgatattttt gatgtacgat gggattctcc tctttatatt agttttgata tggattgcct
    16021 tgatcctgct tttgctccgg gtatatcaca tcgcgaaccc ggagggcttt ccacgcggga
    16081 agtgttaagt atcatccaat cacttaatgc cgatatcatc ggcgccgaca ttgtcgaata
    16141 caacccggaa atggatatca atagcacgac ggatatggta gcggcaaaag tatttaagga
    16201 aatagctgcg atcatgctgt aggttttctt gcttttttat tttgtaaaga caagaaaacc
    16261 tgatgactgt ttggttaaac gcggaaacgt caaatcaatc attcgatatg agtaagtaat
    16321 aacccaacgc gtagtgagga atctatgaat cggaaacatg tgtgttttgt cggtatgcta
    16381 atgatcctga tgccggggtt tctttttaca cagaaaaaaa cggaagtgcc gagcggcatg
    16441 gaacccgggt tgtacaaatc tatggaatgg cgccaaatcg gtccgtttcg cggaggccgt
    16501 tccgtcgcgg ttgccggtga cgtgcgcgat ccgttgacct tttattttgg tgcgaccggc
    16561 ggcggtgtat ggaaaacgac agacggcggt attacgtggc tcaatgtatc ggacggattt
    16621 tttaaaacca gcgcggtcgg tgcgttaagc gtagccgagt cggatcctaa tgtgatctat
    16681 gccgggatgg gcgaaacgtg cattcgaggt aatattacgc tgggcgacgg tatttacaaa
    16741 tcgcttgatg ccggcaaaac ctggtcgcat accggtttga aagaaacgca gtttatcggc
    16801 ggcattgcga tacatccgca gaatcccgac ttcgttttag tcgccgcaat gggacatatt
    16861 tttggtacca ataaagagcg cgggatttat aaaacaaccg atggcggaaa aacgtggaaa
    16921 caaactttat acaaagacga taaaaccggc gcggtcgaag tggcgcttga tccacgcaac
    16981 ccgcgcatcg tatatgcggc gttatgggaa gctcatcgca atgcgtggag catgaccagc
    17041 ggaggttcgg gttcggggtt atataaatca accgacggcg gcgaaacgtg gacggatatt
    17101 tctaaatatc ccggcttgcc taaaggcgtg atcggaaaaa tcggtatttc cgtttcacct
    17161 gtcaaagacg gactcgtgtg ggcatgtatc gaaaatgata acggcggtgt ttttagatcg
    17221 gatgacggtg gtcagacatg gcggcgcacc aatgaagaac gaaatttgcg ccagcgggca
    17281 tggtattatt cacatattta tgccgatcct gtcaattcgg aatcggtgta tgcgctcaat
    17341 gtacagtttt tcaaatccgt ggatggcggg cgaacgttta aaaattttga aaccaaacac
    17401 ggcgatcatc acgatttgtg gattgatcct cgtaattccc aacgaatgat catcgctgat
    17461 gacggcgggg ccgcggtaag ctacaacggc ggtgtatcgt ggaccgattt ggatattccg
    17521 acagcgcagt tttatcatgt atccgtggat aatcaatttc catataacat ctacggtgct
    17581 caacaagaca atagtaccgt tcgtatcagc agtcgtacca cgagttacgg cattgacttc
    17641 gccgattgga gtatcgtcgc aggaggagaa agcggttatg tcgttgccca tccctcgaag
    17701 ccgacggtga ctttcggagg aagctacgga ggatacctta cacgattgga cgcgaccaca
    17761 aaacaagaac ggaacattgc cgtatggcct gacaatccga tcggtgcggg tgccgctgat
    17821 caaaaatatc gtttccaatg gacgtacccg attgtttttt caccgcacga taaaaatacg
    17881 ctatatgtaa cggcgcagtg cgtatttcga tcgacggatg agggtcagag ttggacggtc
    17941 atcagcggcg accttacgcg caacgatcaa tccaaacaac tatcgtccgg cgggccgatc
    18001 accaaagaca acaccagtgt cgaatattac aatacgattt tttcgctggc ggaatcgtac
    18061 aaagaaaaag gagtt
//
LOCUS       DAOQGF010000068        17490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1501_length_17490_cov_5.856897, whole genome shotgun sequence.
ACCESSION   DAOQGF010000068 DAOQGF010000000
VERSION     DAOQGF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17490
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_17490_cov_5.856897"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="coaE"
                     /locus_tag="PK937_10135"
     CDS             <1..600
                     /gene="coaE"
                     /locus_tag="PK937_10135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNH32972.1"
                     /translation="LIGLTGGIGCGKTEVARIFERLGAIVIDADRLGRHVVESDAAVR
                     SEIRRAFGEAFFDFQGRLQRQLLGTFVFSNPERVKQLNAIVHPHLLQAMRARIDEAQK
                     NNPPMILVDAALIYEADIADTFDKVIVVTADEKIRMTRIMTRDQIQSEDAMLRIRAQM
                     PIEEKERRADYVLTNNTSLVDLNRKVEVLFDTLVNNNKR"
     gene            670..999
                     /locus_tag="PK937_10140"
     CDS             670..999
                     /locus_tag="PK937_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNH32973.1"
                     /translation="MIEQLLNVGKRGKGALFKELLNLFQKNAPPSIAKIKSFLELRDG
                     LSLSHEAHSLKGSSANLGAKHLAEICQTIEVLGKESRLQEASEKISELDNVYLESLEE
                     LKKLIPV"
     gene            1060..2367
                     /locus_tag="PK937_10145"
     CDS             1060..2367
                     /locus_tag="PK937_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNH32974.1"
                     /translation="MERFTGLLGVAAIIGIAFLISNNKKRVNWRLVGMGFLIQMVFGI
                     LVIKGHALGFPFNLVAVGFEKIGAGIVAILGYTTKGAQFVFGNLALSPGMEGSMGFFF
                     VFQVLPTIIFFSSLMSVLYYLGIMQKVVQGMAWVMAKLMGTSGAESLSNTANIFVGQT
                     EAPLMIRPFIKTMTNSELYTIMVGGMATIAGGVMAAYIQMLGKSYADVHGVGVEQGQI
                     LFATQLLGASIMAAPAGLLISKIIFPEVEEPVTKGSVKMDVEKNASNVIEAAAGGAAD
                     GLQLALNVAGMLLAFIALIALINGLAGWVGGLIAIPDLSLQMIFGYILAPIAFISGVP
                     WSEAMQVGSLLGTKIALNEFVAYSDMAGMIKSGLLTSDKAIMIATFALCGFANFSSIA
                     IQIGGISPLAPERRKDIAALGLKAVLGGALATLLTGTIGGILL"
     gene            2381..4456
                     /locus_tag="PK937_10150"
     CDS             2381..4456
                     /locus_tag="PK937_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNH32975.1"
                     /translation="MNLEKWRRYKIHVTAFLLVFILTAVFGAPKLAHYMIRTKISETA
                     DRFAARTGYRVMIDSLNFPTASRLTVQSLVLIGDSSHTSGYKELLRLERVTLDFYLWG
                     FLTGNYIRALQMDSVHIVSTRSLAGQNNWLQFWQSWRGQNIPADTNAKPQVNLIERWV
                     TAYLDKKLPSIEIRHASWIHLEERTVWKKRKPVSEWGESLRLDEMHISLKESLFRDAE
                     FDLTGTVYRGKQKNGVTISGTMDHQDHALYVKSFFDSDFRIPFLENWTDAVIALKGVE
                     FKIHSIEGYGNQNTLKAKLDIQNLDIVSESLSDEKLKDINVGFDLHITADPDSIIIHP
                     ETRVYLNTIHAFFSGVAKPFRKFPVWDVTMRMPPTTMDDFLSSIPKPIIRRLDGMRVK
                     GQFDYQARFRLDLAQIDSIRFDPALHISSDFKVETYGDSIHVYALRDTFLYHFKTEME
                     KDSMVWVGPLNAYYMPYDSIPPLVMQAVIACEDNSFFKNDGFNVLQIERSMRDNIREK
                     KFARGASTISMQLVKNIFLSREKTISRKFQEMMITWLMNHDLALDSLKKNKEKHKKRL
                     MEIYLNIVEWGPDVHGIAHAAEFYFKKKPKELTVPEAVFLATILPNPKKYERYFESGK
                     SKKKHQDFMNLIVRMLEDKKVIDSVAMQAAIPVTFTVRGSAQRWIKDYALHDSTDSDM
                     EFSLPTIRE"
     gene            4478..4855
                     /locus_tag="PK937_10155"
     CDS             4478..4855
                     /locus_tag="PK937_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32976.1"
                     /translation="MNQIINGTGASLHGIEQACITGNGSLSEACRDGAIQVYVAFRNK
                     INMSCSLVDVHSLDGLHDSVAVKIQSSRISPAEIYDRLASGQPSIMGYISQNSFHLDF
                     RYIRKEEVADLLRRLKIVLEIES"
     gene            4895..5437
                     /locus_tag="PK937_10160"
     CDS             4895..5437
                     /locus_tag="PK937_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32977.1"
                     /translation="MKSFIVLLTVIGVIVSSCGPQKATRSDMSKLDEARANAEKAKEN
                     LANTQNASQMAADELAKKKDENEKSKDELRKVRANLKKMGQWADYMPFNKENNTLLLP
                     GGVTRTIRKEITDTEALTQLNAMLDAQATKQDKDHHALTTCIGGFETCLNGKIVVLYF
                     TKDDAGACKRTWYETENNCD"
     gene            5456..6844
                     /gene="fumC"
                     /locus_tag="PK937_10165"
     CDS             5456..6844
                     /gene="fumC"
                     /locus_tag="PK937_10165"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNH32978.1"
                     /translation="MSFRIETDSMGEVQVPSDRYYGAQTARSLSNFKIGGDRFPREMI
                     RALGILKKSAAIANQELGTLTKDKAELIIKACEEVIAGKLDDHFPLVVWQTGSGTQTN
                     MNANEVISNRAIEISGGTMGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAERIVHHLI
                     PKVKLLRTTLQQKSDAFKDVIKIGRTHLMDAVPLTLGQEFSGYVQQLDNNVKRLEQVL
                     DGLYELALGGTAVGTGLNTHPKFAETGAKHIAQITGLPFRSAPNKFEALAAHDAIVFA
                     SGATKTLAASLMKIANDVRWLASGPRCGIGEISIPDNEPGSSIMPGKVNPTQCEAMTM
                     VCAQVMGNDAAINVAGASGNFELNVYKPVLIFNLLNSIRLLGDACDSFNEHCAVGIEP
                     NTNVIKKHLDNSLMLVTALNPHIGYDNAAKVAKKAFHDNITLKEAAIQLGLLTSEKFD
                     EVVRPEKMINPS"
     gene            6853..7221
                     /locus_tag="PK937_10170"
     CDS             6853..7221
                     /locus_tag="PK937_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32979.1"
                     /translation="MPRITFNFGWLLFAIGIAGYVASGMSSTTALIPSIFGIAFVLLG
                     WIAMQKESMRKHLMHAAAILGLLGIIGTFKGMLRFFAMLGGTEVERPIAVTFQSVMAM
                     LCIVFVALCVHSFIKARTGK"
     gene            complement(7368..8246)
                     /gene="arsM"
                     /locus_tag="PK937_10175"
     CDS             complement(7368..8246)
                     /gene="arsM"
                     /locus_tag="PK937_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNH32980.1"
                     /translation="MVNDEKLKEVVREKYGQIALTTLDDNAGSCCGVGGCGTIDYNIM
                     NEDYSQLDGYNPDADLGLGCGIPTEFAQIRAGQTVVDLGSGAGNDAFVARKLVGPSGF
                     VIGVDMTPAMIDRARQNNEKLGYKNVEFRLGEIENMPVASNSVDVVVSNCVLNLVPNK
                     LNAFAEVHRIMKPGARFSISDIVLDGKLPANIAKAAEMYAGCVSGAIQKHEYIDIVRK
                     LGFTDIQLVKDREINLPDDILAPYLNKEEITEFRKSGARVVSITVVAQKSTQVQTGCC
                     SDGNVAKSKAGGCGCN"
     gene            complement(8267..8587)
                     /locus_tag="PK937_10180"
     CDS             complement(8267..8587)
                     /locus_tag="PK937_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH32981.1"
                     /translation="MAQSKALEFKQEEILLAEFAKALAHPARIAILKELARRSECICG
                     DLVEVLPLAQSTVSQHLKELQKIGLIQGEIDGPKSCYCINKKNMDKYLSILNKFFNHI
                     ESCC"
     gene            8743..9723
                     /locus_tag="PK937_10185"
     CDS             8743..9723
                     /locus_tag="PK937_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNH32982.1"
                     /translation="MKPHNSISQFMDHHYRHFNSAAMMDASKAYIKHLENGGQMFVTL
                     AGAMSTAELGLSLAEMIRQDKVHAITCTGANLEEDVFNLVAHDHYVRVPHYRDLTPQD
                     EQKLLDRHLNRVTDTCIPEGEAMRRIESAVLEEWVKADKAGERFFPHEFMYKILLSGK
                     LEKYYQIDPKDSWMMAAAKKNLPMFVPGWEDATLGNMYAGHVISGDVKNVHTVRTGIE
                     YMMRLADWYTERSKEHSVGFFQIGGGIAGDFPICVVPMLHQDLQRTNVPLWGYFCQIS
                     DSTTSYGSYSGAVPNEKITWGKLGIDTPKFIVESDATIVAPLMFAYILGW"
     gene            9799..10500
                     /locus_tag="PK937_10190"
     CDS             9799..10500
                     /locus_tag="PK937_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32983.1"
                     /translation="MKRNHYWIGLVFALSIISCGGDSETSITRDGDILIIGDGATDLS
                     LDTILSANNLTFSTVPMDSGFYGDELNSENISTVILCDGEGYEFGMPEQGQQALLDFV
                     ANGGRLILMEWIAYEIDSDRYTVLDTIVPVLRSSGREGTETYTVQLFNELTEGLDTTF
                     TVSSGSNIGTARFGTVHIKGSSSGDALVTHTWGQGEVIQMACAGNYEGYNPFAEPNMK
                     KLILNAVMISSLPKK"
     gene            complement(10610..11125)
                     /locus_tag="PK937_10195"
     CDS             complement(10610..11125)
                     /locus_tag="PK937_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNH32984.1"
                     /translation="MLSKKLQDAFNEQINREFFSEYLYLSMSAWCKSGNMDGIGNYFH
                     VQAQEEHQHAMKFFNFIHDRGGTVTLKALDAPQSKFKSLIEIFEQTLEHEQFITKSIN
                     TLMSTALKEDDHAAVSFLKWFIDEQVEEEASVSKILHKIKLIKGEGHGLLMIDAELGQ
                     RKFTPDTGNNE"
     gene            complement(11145..11942)
                     /locus_tag="PK937_10200"
     CDS             complement(11145..11942)
                     /locus_tag="PK937_10200"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HNH32985.1"
                     /translation="MEGIIIGLVCFGIAVLLLYFRKKNQDKLLEIKFVKTSTAQELKE
                     ICKQVNDELSTTGAFRQQTEVKGVIQCAHPITSELAKQPCVWYEMSVDERYEETYTER
                     DQNGREVRRTRTGTENVASNSQRVHFEVEDATGRITINPNDADIEGTQVLNRYEQNFS
                     GGRIQLGGFSMNINIGRSGRRILGYEFTESLIPLDRRVYVLGEASDASGELMVQKPED
                     KEKPFIITLKSEEELTRGTESTIRNLMIGSIVLVVIGLVAIAYGIMK"
     gene            complement(11958..12536)
                     /locus_tag="PK937_10205"
     CDS             complement(11958..12536)
                     /locus_tag="PK937_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32986.1"
                     /translation="MTDITIYTFFPLIFTTIFWHEAGVIFWFRALESDFGHWTNAAAA
                     TLVIVSVIDFFWLKIMFFLFRTSFQRIGQTAWSHRIIARLEETKWFPRFRGFFVKKEI
                     DHQTAETPDETSGFKRFIKKTGYWGILFCGSLPGPGVKEIGIIMALTPKYRDRGFMLM
                     YIGGVIKTIMTMLVYGGLYNLIEHYLRNQIAF"
     gene            12625..13014
                     /locus_tag="PK937_10210"
     CDS             12625..13014
                     /locus_tag="PK937_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32987.1"
                     /translation="MKTLLMAVCFFVLQDQPAFDPSLMYGRWKWYASEGGLMEQRITV
                     EQTQQNKSLLFTKDHAVFYMVGDSVSYKSRYVTEMHRSEFTGQDTPLLRIQGISKDQW
                     ITMQGKDSLILRENKYDGWTHRYVRLH"
     gene            13139..15145
                     /locus_tag="PK937_10215"
     CDS             13139..15145
                     /locus_tag="PK937_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNH32988.1"
                     /translation="MAEKKNTNRFDNLEIQPRSHSETVAHPAPGSTKNERFEWIEVPQ
                     QKTFTKRFNTKARSITSKRCPRCGWLDQSAVTECFRCGYDFQTNDMHLDFFKQEKIDL
                     PPVEIKVDMGFQDVLSKKQYDTLDAYQLRLEAERLNLISGFDRLIAVNDLNITHYDYQ
                     INTALEALRRMRGQVLLADEVGLGKTIEAGIITKELIERNLAKNILILTPASLVGQWQ
                     DELLMKFGENFIVPQQESDWRAPKVIASIDMAKRNEAANILLSRDFDLLIVDEAHRLK
                     HRSTIGFKFVNKIRKKYVLLLTATPVHNDLTELYSLVTILKPGLFGTIRSFKRKFMLK
                     DDPRLPNNEDQLKHILQDVMIRNRRDKVGINLPPRKAAIYHLDLTPIESELYREVTNY
                     VREEFRREANFDVHILSLITLQREVCSSPQATRHTLINFMNRNYPAKTRERLAYFVEL
                     CDAVKISRKSAATIELLKKFPSKTIIFVEFIETMKHLQMELEQEGISVELFHGGLSGT
                     HARKEAIDRFRKSKQVLISTQAGGEGLNLQFCSQIINYDLPWNPMRVEQRIGRVHRLG
                     QERDVFVFNLSVTDTIESRILELLANKIRMFELVIGELDIILGDIETEKNFEQQLMEI
                     FMRSTDEKEMNQQLERFGNQLTHVRRQYDKVRENSEILSDIVDV"
     gene            15218..16783
                     /locus_tag="PK937_10220"
     CDS             15218..16783
                     /locus_tag="PK937_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HNH32989.1"
                     /translation="MQKKRIIWADDEIELLKPHILFLEQRGYEVTPVTNGEDAISQVR
                     TRKYDLVLLDEMMDGKDGLTTLEEIKENSPTIPVVMITKSEEERLMEDAIGRKIDFYL
                     TKPVNPSQILMACKQIFESRKLTEQKISRDYVQEFNRISQKLMDRLEFKDWYDIAQSM
                     AEWEVELDALTDTGLRQMLEDQRKACNVEFGKFVEKNYHGWVNSGLPQRPEFSVDIVR
                     NYVFPKIAARRKVVFIVIDCLRIDQWLGFESVLQEYFNINKKFYYSILPTSTPYSRNA
                     IFSGMWPSEIEQKFPDIWSKGEEDDSSRNRFEKELLEYQLKSNRVPMETEAKYVKILN
                     AEHSRQLETNITSFVNSSQLTAIVLNFLDILAHTRSDSHVIKEIAPHEPAYRSLSNSW
                     FTHSSFLEILKTLSRLDCQIVITSDHGSIRSLHGTEVIGDKETSTSLRYKYGKNIKSN
                     PKHSMFIKNPLEYKLPKRGVNTHYIIAKEDYYFLYPTNYHHFLNHYNDTFQHGGISVE
                     EVIMPVIEMEGKR"
     gene            complement(16865..>17490)
                     /locus_tag="PK937_10225"
     CDS             complement(16865..>17490)
                     /locus_tag="PK937_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNH32990.1"
                     /translation="NVSFYNESRNNAVFPTPTIGMIGIIEDLKHVTTSWFKSEGDKIF
                     LIGKNGTDIGGSEYLKTIHGKISGDAPSLDMAYEKKVQDFVLDTIIKGWVRSAHDVSD
                     GGLAVALAESCYQGKPGHTIGATIRLNDALRPDFLLFSETQSRIILTTDTENAERLKQ
                     WAQTQHVDCVEIGVVGGKTLRINDWIVMQASDIERLHRGAIRTTMTK"
BASE COUNT         4915 a         3655 c         4050 g         4870 t
ORIGIN      
        1 ttgatcggtc tcaccggcgg tatcggatgc ggcaaaacgg aagtagcgcg tatttttgaa
       61 agactcggtg caatagtgat tgatgccgat cggcttggcc gtcatgtcgt ggagtcggat
      121 gcggcggttc gatcggaaat acgacgcgcg tttggtgaag ctttttttga ttttcaggga
      181 cgtttgcaaa gacaactgtt gggtacattt gttttttcaa atcctgaacg tgtcaaacaa
      241 ctcaacgcca tcgtgcatcc gcatttactg caagccatgc gtgctcggat tgatgaggcg
      301 caaaaaaaca atccgccgat gatattggtg gatgccgcat tgatctatga agccgatata
      361 gctgatacct ttgacaaggt gatcgtcgtg acggcggatg aaaagatacg gatgacacgg
      421 atcatgacgc gggatcagat tcaatcggaa gacgcgatgt tgcgcatacg cgcacaaatg
      481 ccgatcgaag aaaaagaacg tcgcgccgat tatgtgttga ccaataatac atctttggtt
      541 gatctgaatc gaaaagtaga agtattattt gatacgttgg tgaataataa caagcgataa
      601 atttatattt aaaactcatc ttaacgcatg gctgttccac atcatcgcac ggatctcatc
      661 gaatacgcaa tgatcgaaca attgctgaat gtcggtaaac gtgggaaggg tgcgcttttc
      721 aaagaattat taaatttgtt tcaaaaaaat gcgccgccca gtattgcaaa aattaaatca
      781 tttctggaac tgcgtgacgg attgagttta agtcatgaag cacacagcct caaaggttcc
      841 agcgccaatt taggcgcaaa acatcttgca gaaatttgtc aaactataga ggttttaggt
      901 aaagagagtc gtttgcaaga agcctctgaa aaaatttcag aattagacaa tgtttatctt
      961 gaatccttgg aagagttaaa aaaattaatt ccggtttagg tcggcgaaat tagacttaaa
     1021 gagatatgtt ttaacaaata aataacttcg ggagtgcaca tggagcgatt tacgggactt
     1081 ctcggtgtag cagcgatcat cggtatagct tttttgatca gtaataataa aaagcgggta
     1141 aactggcgat tggttggcat gggtttttta atccagatgg tctttggcat acttgtgatc
     1201 aaagggcatg ctttaggatt tccgtttaat ctcgtagccg taggatttga aaaaataggc
     1261 gcgggtattg ttgcaatttt gggttatacg acgaaaggtg cacaatttgt ttttgggaac
     1321 cttgcgttaa gcccgggaat ggaaggcagc atgggtttct tttttgtgtt tcaggttttg
     1381 cccacgatta ttttcttttc gtcgttaatg tcggttctat attatctcgg tatcatgcaa
     1441 aaagtagtac aaggtatggc ttgggtaatg gccaaactga tgggcacgag cggagcggaa
     1501 tcgctttcca atacggcaaa tatttttgtc ggtcaaaccg aagcgcctct gatgattcga
     1561 ccatttatca agaccatgac caattcagaa ctgtacacca tcatggtcgg gggtatggcg
     1621 accattgccg gcggtgtgat ggcggcctat atccagatgc tgggtaaatc gtatgccgat
     1681 gtgcacggcg tgggtgtcga acagggtcag atattatttg cgacgcagct tctcggtgca
     1741 agtattatgg ccgctccggc cggtttattg atttctaaaa ttattttccc ggaagtggag
     1801 gagcctgtga cgaaaggttc cgtcaaaatg gatgttgaaa aaaatgcatc caatgtgatc
     1861 gaagccgcgg ccggcggcgc cgctgacggg ttgcaactgg cgctcaatgt tgccgggatg
     1921 cttttagctt tcatcgcgtt gatcgcattg atcaatggtt tagccggatg ggtcggcggg
     1981 ctcattgcga ttccggatct atccctgcaa atgatttttg gatatattct tgcgccgatc
     2041 gcgtttattt ccggtgtgcc gtggagcgaa gccatgcagg tcggcagttt acttggaacg
     2101 aaaatcgcat taaacgaatt tgtcgcttac tccgatatgg ccggcatgat caaaagcggg
     2161 ctgcttactt cggataaggc catcatgata gctacgtttg cgttatgcgg ctttgccaat
     2221 ttttcttcaa ttgccattca gatcggcggt atatcaccat tggcgccgga gcgtcgaaaa
     2281 gatattgcgg ccctaggact aaaggctgtg ctcggcggcg cgttagcgac attattaacc
     2341 ggaacgatcg gcggaatttt gctctgaaga aagtgtactc atgaatttgg aaaaatggcg
     2401 ccgttataaa atacatgtaa cggcgtttct tttagtattt attctcaccg ccgtgttcgg
     2461 agcgcccaaa cttgcgcatt atatgatccg aactaaaata tccgaaacgg cggatcgctt
     2521 cgctgcacgt acgggttacc gtgtaatgat cgacagtctt aactttccga cagcctctcg
     2581 attaaccgtg cagtcgcttg tactgatcgg tgattcatcg catacttccg gttataaaga
     2641 attgctgcga ttagagcgag tgacattgga tttttatctt tggggatttc tcaccggtaa
     2701 ttatatccgt gcgttgcaaa tggactcggt tcatattgtt tctacacgca gtttggccgg
     2761 tcagaataat tggcttcaat tttggcaatc gtggcgaggt caaaacattc cggccgacac
     2821 caatgcgaag ccgcaagtga atctgatcga acgctgggtg acggcgtatt tggacaagaa
     2881 attaccgtct atagaaatcc gtcacgcgtc ttggattcat ctcgaggaac gaacggtgtg
     2941 gaagaaacgt aaaccggtat ccgaatgggg tgaaagcctg cggctggacg agatgcacat
     3001 cagtcttaaa gaaagtctgt ttcgggatgc tgaatttgat ttgaccggta cggtatatcg
     3061 cggaaaacaa aaaaatggcg ttacgatatc cggtaccatg gatcaccaag atcatgcgct
     3121 atacgtcaaa agtttttttg attccgattt ccgaattcct tttttggaaa attggaccga
     3181 tgccgtgatt gcgctcaagg gcgttgaatt taaaatacac agcatcgaag gatatggtaa
     3241 tcagaatacg ctcaaagcca aattagatat acaaaacctc gatattgtat cggaatcgct
     3301 ttcagatgaa aaactaaaag acattaacgt cggttttgat cttcacatta cagcggaccc
     3361 ggacagtatc attattcacc ccgaaacacg ggtttacctc aatacgattc atgccttttt
     3421 ctccggtgta gccaaaccat ttagaaaatt tcccgtgtgg gacgtcacga tgagaatgcc
     3481 gcccacaaca atggatgatt ttttaagttc gatacctaag ccgataatac gtcgcttaga
     3541 cggaatgcgt gtaaaaggtc aatttgacta ccaagcacgg ttccgccttg atttggcgca
     3601 gattgacagc atccggtttg atcccgcatt gcatatcagc agtgatttta aggttgaaac
     3661 atacggcgat tccattcatg tttacgcatt gcgcgatacg tttttgtacc attttaaaac
     3721 cgagatggaa aaggacagca tggtatgggt cggtccgcta aatgcgtatt acatgcctta
     3781 cgacagtata ccgccgctgg tcatgcaggc cgtcattgcg tgtgaagaca attccttttt
     3841 caaaaatgac ggattcaatg tgcttcagat cgaacgttct atgcgggata atatacgtga
     3901 aaaaaaattc gcacgcggcg ccagtacgat ttcgatgcaa ttagttaaga atatttttct
     3961 gagccgtgaa aaaacgatta gccgcaaatt ccaagaaatg atgatcacct ggctgatgaa
     4021 tcacgacctg gcgctggatt cgctgaaaaa aaacaaagaa aaacataaaa aacgattgat
     4081 ggagatatac ctcaatatcg tagagtgggg gcccgatgtg cacggtatcg cgcacgcggc
     4141 ggaattttat tttaagaaaa aaccgaagga attgacggta cccgaagcgg tgtttttggc
     4201 tacgatattg cccaatccca aaaagtatga acgctatttt gaatccggca aatccaaaaa
     4261 gaagcaccag gattttatga acctgatcgt gcgtatgctg gaagataaaa aagtaataga
     4321 ttcagtggct atgcaggcgg ccattccggt tacatttacg gtgcgcggtt cggcacaacg
     4381 atggattaaa gactatgccc tgcatgattc gacggacagc gatatggaat tttcattgcc
     4441 aacgatcaga gaataaaaat tttatcggac catgcgaatg aatcaaataa tcaatggaac
     4501 gggggcatca ctacacggta tcgaacaagc gtgtatcacc ggcaatgggt ctttatcaga
     4561 agcctgccgt gatggagcga ttcaggtgta cgtagctttt cgtaataaaa ttaacatgtc
     4621 ttgttcgctt gtggatgttc attctttaga cgggcttcat gacagtgttg ccgtcaaaat
     4681 acaatcttcg cgaatatcac ctgcggaaat ctatgatcgc ctcgcttcag gccaaccgtc
     4741 cattatgggg tatatttcac aaaattcatt tcatcttgat ttccgttata ttcgcaaaga
     4801 agaggtggcg gatttgcttc gtagattaaa aatagtatta gaaatagagt catagaacca
     4861 gtatgtttat acattatttt acggagaaat aaacatgaaa tcattcatag tattattgac
     4921 ggttatcggt gtgatcgtat cctcatgtgg cccccaaaaa gcaactcgat cagatatgag
     4981 caaactggat gaagcgcgtg ctaatgcgga aaaagcaaaa gaaaatttag ctaatacgca
     5041 aaatgcttcc cagatggccg ccgatgaact ggccaagaag aaagacgaaa atgaaaaatc
     5101 taaagatgaa ctgcgcaaag tgcgtgctaa tctgaaaaag atgggtcaat gggccgatta
     5161 tatgccgttc aataaagaaa ataatactct tttactgccc ggcggagtta cacgcactat
     5221 tcgtaaagaa ataacggata cggaagcctt gacccaactc aatgctatgc ttgatgcgca
     5281 agcgaccaaa caagataaag atcatcatgc gttaactaca tgcattggcg gatttgagac
     5341 atgccttaac gggaaaattg ttgttttata ttttaccaaa gatgatgccg gtgcgtgcaa
     5401 acgcacgtgg tatgaaaccg aaaataactg cgattaaatt caggagaaat acgacatgag
     5461 ttttcgaatt gaaaccgaca gcatgggtga agtacaagtc ccgtcggatc gttattatgg
     5521 cgctcaaacg gcgcgttcac tttccaattt taaaataggc ggcgatcgct ttccgcgtga
     5581 aatgatacgt gcactcggta ttttgaaaaa atccgccgcg atagccaatc aggaactagg
     5641 gacgttgacc aaagataaag ccgaattgat catcaaagcc tgtgaagaag tgattgccgg
     5701 aaaattggat gatcattttc ctttggtcgt atggcagacg gggagcggca cacaaaccaa
     5761 tatgaatgcc aatgaggtga tttccaatcg cgctattgaa atttccggcg gcacgatggg
     5821 cagtaaaaaa cccattcatc ctaatgatga tgtaaataaa gcccagtcgt ccaatgatac
     5881 atttcctaca gccatgcata tcgccgccgc agaacgcatc gtgcatcact tgattcccaa
     5941 agtaaaattg cttcgcacga cactccaaca aaagtcggat gcgtttaaag atgtgatcaa
     6001 aatcggacgc actcacctca tggatgctgt tccgttgaca ttgggacaag agttttccgg
     6061 ttatgtccaa caattagata acaatgtcaa gcgacttgag caggtactcg acggattata
     6121 cgaactggct ttgggtggta cggcggtcgg aacaggcctt aatacgcatc ctaagtttgc
     6181 ggaaaccggt gcaaaacata tcgcacaaat tacgggtctg ccgtttcgca gtgcgccgaa
     6241 taagtttgaa gcattagcgg cgcatgatgc gatcgtgttt gcgagcggcg caactaaaac
     6301 attggcggca tcgctgatga aaatagccaa tgacgtacgg tggctggctt ccggcccgcg
     6361 ttgtgggatc ggtgaaatat ctataccgga taatgaaccc ggaagttcca tcatgcccgg
     6421 taaagtcaat cccacgcagt gcgaagccat gaccatggtg tgtgcgcagg tgatgggtaa
     6481 tgatgccgca atcaatgttg ccggcgcttc aggtaatttt gaactaaacg tatataaacc
     6541 ggtactgatt tttaatcttc tgaattcaat tcgtttactt ggtgacgcct gcgattcgtt
     6601 taatgaacat tgtgctgtcg gtattgaacc taacaccaat gtgatcaaaa aacatttaga
     6661 taactccctc atgttagtca cggctttaaa tccacatatc ggttatgata atgcagccaa
     6721 agtcgctaag aaggcgtttc atgataatat caccctcaaa gaagcggcga ttcaattggg
     6781 tttgttaacg tcagaaaaat ttgatgaagt tgttcgtccc gaaaaaatga ttaacccatc
     6841 ctaaggagat ttatgcctcg tatcactttc aattttggtt ggttgctttt tgcgatcggt
     6901 atagcagggt atgtcgctag cggtatgtcc agcacgacgg cgcttattcc gtctattttc
     6961 ggaattgcgt ttgtgctctt aggttggata gccatgcaaa aagaatccat gcgtaaacat
     7021 cttatgcacg ccgccgcaat actgggttta ttgggcatta tcggcacatt caaaggcatg
     7081 cttagatttt ttgcaatgtt aggcggtacg gaagttgaaa ggcctatagc cgtgacattt
     7141 caatcagtta tggctatgct ttgtattgta tttgtagcct tgtgtgtaca ttcttttatc
     7201 aaagcacgca caggtaagta gattgtagaa atagaaaagg ctgtttgaca gttcacatca
     7261 aaaccttgcg aagatcgttt ttaataacga atcgtcaatt ttatgtgaat aaaagtttct
     7321 catcgttaaa tatgagagct cacgtcaaac agcctttttc ttttttttta attacagccg
     7381 caaccgccgg ctttggattt ggcaacattg ccatccgagc aacaaccggt ttgcacttga
     7441 gtactttttt gcgctactac cgtgatgctt acgacgcgtg cgccggattt acggaattcc
     7501 gtaatttctt ctttattcag atacggagcc aaaatatcat ccggcaagtt tatttcacga
     7561 tctttgacta actgaatatc cgtgaaaccg agtttgcgga cgatatcaat atattcgtgt
     7621 ttctgaatcg caccggaaac gcaaccggca tacatttcgg ccgccttggc gatattggcc
     7681 ggaagcttgc cgtccaaaac gatatccgaa atagagaaac gtgcgccggg tttcataata
     7741 cgatgtactt ccgcaaaggc attgagttta ttaggtacaa gattgagtac gcagttactc
     7801 acgacgacat ccacgctgtt tgaagccaca ggcatatttt ctatttcacc caggcgaaat
     7861 tccacattct tataacctaa tttttcgttg ttttggcgtg cacgatcaat catagcgggc
     7921 gtcatatcca cgccgatgac aaaaccggac gggccgacta attttcgtgc aacgaaagca
     7981 tcgttacctg cgccggaacc caaatccacg acggtctgac ctgcacggat ttgagcaaat
     8041 tccgtaggaa taccgcaacc tagaccgaga tcggcatcgg gattgtaacc atcaagctgt
     8101 gaataatctt cattcataat attgtaatca atagtgccac agccaccgac gccgcagcaa
     8161 gaaccggcat tgtcgtccag tgttgtcagt gcgatctgac cgtatttttc acgaacaact
     8221 tcttttagtt tttcatcatt taccatagtt actcctttgg tttttgttaa caacacgatt
     8281 cgatgtgatt aaaaaatttg ttaagaatgc ttagatactt gtccatgttt tttttgttga
     8341 tacagtagca agatttcggt ccgtcaattt caccttgaat caagccgatt ttttgtaatt
     8401 cctttaaatg ttgggaaacg gtggattgcg cgagcggtaa cacctcaacg aggtctccgc
     8461 atatacattc gctgcgccgg gccagttctt tgagaattgc aattcgcgcc ggatgggcga
     8521 gtgctttggc aaattcggcc aataagattt cttcttgttt aaattcgagc gctttacttt
     8581 gtgccataat ttttctttat cgtttatcgt aaatatacga tataataatt caaaatgttt
     8641 catttgtcaa gcaggattta aaaaaaactt ttattttttt cataaatggt tatcttaagc
     8701 gcacggaaat cattgataaa catttacata caggataaaa aaatgaaacc acacaactcg
     8761 attagtcaat ttatggatca tcattaccgc cattttaatt cggcggctat gatggatgcc
     8821 tccaaagcgt acatcaaaca tctcgaaaac ggcgggcaga tgtttgttac gttggctggg
     8881 gccatgagta cggctgaact cgggctatcc ttggcggaga tgatcaggca ggataaagtt
     8941 catgcgatca catgcacggg tgccaatttg gaagaagatg tttttaatct tgttgctcat
     9001 gatcactatg ttcgtgtgcc gcactatcgt gatctgacac cgcaagacga acaaaaatta
     9061 cttgatcgtc atttgaatcg cgtcacggac acctgcattc ctgaaggtga agccatgcgg
     9121 cggatcgagt cagctgtatt ggaagagtgg gtaaaagccg ataaagccgg tgaacgtttt
     9181 ttccctcacg aatttatgta taaaattctc ctcagcggaa aattagaaaa atattatcag
     9241 attgacccca aagatagctg gatgatggcc gcggctaaga aaaatttgcc tatgttcgtt
     9301 cccggttggg aagatgctac attgggtaat atgtatgcgg gtcatgtcat cagcggcgat
     9361 gtaaaaaatg ttcataccgt acgcacgggg atcgaatata tgatgcgact ggccgattgg
     9421 tacaccgagc gttccaaaga acattccgtc ggtttctttc aaatcggcgg cggcattgcc
     9481 ggtgattttc cgatctgtgt tgttcccatg ctgcatcagg acctgcaacg taccaatgtg
     9541 cccctgtggg gatatttctg tcagatcagc gattcgacga cgagttacgg ttcatactcc
     9601 ggcgctgtgc ccaacgaaaa aatcacatgg ggaaaactcg gtatagacac gcctaaattt
     9661 atcgtcgaat ccgatgcgac gattgtggca ccgctgatgt ttgcgtatat tttgggatgg
     9721 tgatttgcgg cgaagttgca ttctgcgaaa agttttgatg agggttcggt tctcatttat
     9781 ttaaaagagg agaagagtat gaaaagaaac cattattgga ttgggcttgt tttcgcatta
     9841 tcaattattt cctgcggcgg agatagcgaa acaagtatca cacgcgacgg agatatactg
     9901 atcatcggtg acggtgcaac ggatttgtct ctggatacga ttttatctgc taacaatttg
     9961 acattttcta ctgtaccgat ggattccgga ttttacggcg acgaattaaa ttccgaaaat
    10021 atttcaacgg ttattctgtg tgatggtgaa ggatacgagt ttggtatgcc cgaacaagga
    10081 caacaggctc tgcttgattt tgttgctaac ggaggtcgtc tgatccttat ggaatggatc
    10141 gcctatgaga ttgatagtga tcgctataca gtgcttgata ccattgttcc agttttaagg
    10201 tcaagcggcc gtgaaggcac agaaacatat accgtgcaat tgtttaacga attgacggag
    10261 gggttggata ctacgtttac cgttagtagc ggatccaata ttggaacggc acgttttgga
    10321 actgtgcata ttaaaggaag cagttctggg gatgcgctgg tcacccatac gtggggacaa
    10381 ggcgaagtaa tccaaatggc ttgtgccggc aattatgaag gctataatcc gttcgcggag
    10441 cctaacatga aaaaattaat tctcaatgct gtgatgattt cttcactgcc taaaaagtga
    10501 tcaatatttc ttatttaatt gatcaattaa tatgatttat ttgcatgaaa aaagcctccg
    10561 tcaacatacg gaggctttta ttatttggta aatcccagtt atgtgaagct cattcgttgt
    10621 tgcctgtatc cggtgtaaac ttccgttggc caagctctgc atcaatcatc agtaacccgt
    10681 gaccttcgcc tttgattagt ttgattttgt gcaggatttt agataccgaa gcttcctctt
    10741 cgacttgctc gtcaataaac cacttcagaa agctcaccgc cgcatgatcg tcttctttga
    10801 gggccgtact catgagcgta ttgatgcttt tggtgataaa ttgttcatgc tccagtgtct
    10861 gttcaaaaat ttcgataagg gatttgaatt ttgattgggg cgcgtctaat gccttgagag
    10921 ttacggtgcc gccacgatca tgaataaagt taaaaaattt catagcgtgc tgatgctctt
    10981 cttgtgcctg cacatgaaaa taatttccga tgccatccat atttcctgat ttgcaccagg
    11041 ccgacatgga gaggtagaga tattctgaaa aaaattcgcg attgatctgt tcgttgaatg
    11101 cgtcttgtaa ttttttactt agcataagta aggcctttct atatttattt catgatgcca
    11161 tatgctatgg cgactagccc gatgacgacg aggacgatac tgccgatcat cagattgcgt
    11221 atggtagatt cggtaccgcg tgtcagctct tcttcggatt tgagggtgat aataaagggt
    11281 ttttctttgt cttccggttt ttgaaccatg agttcgcccg aggcatcgga tgcctcaccg
    11341 aggacataca cgcggcgatc cagtggtatc aatgattcgg taaattcgta gcctaaaatg
    11401 cgtcgtccac ttcggcctat attgatattc atcgaaaacc cgccaagttg gatacgccct
    11461 ccggaaaaat tctgttcata acggttgaga acctgcgtgc cttcgatgtc ggcgtcgttg
    11521 ggattgatag tgatgcgacc ggtcgcgtct tccacttcaa aatgcacacg ttggctattg
    11581 gaggccacat tttcagtccc ggttcgtgtg cgacgcactt cgcggccgtt ttgatcacgt
    11641 tccgtataag tttcttcata gcgttcgtcc acactcattt cgtaccagac gcatggttgc
    11701 ttggccaatt cggatgtgat cggatgagca cattggatca cgcccttgac ttccgtttgt
    11761 tgccgaaatg cgcccgtcgt agatagttca tcgttgactt gtttgcaaat ttccttgagt
    11821 tcctgagcgg tggaagtttt gacaaacttg atttctaata atttatcctg attttttttt
    11881 ctgaaataga gcaaaagaac ggcaatgccg aagcaaacca gcccgatgat aatgccttcc
    11941 ataaaacgcc tcccaagtta aaaagcgatt tggtttcgca aataatgttc gatcaaatta
    12001 taaagcccac cgtacacaag catagtcatt atcgttttga tcacaccgcc gatatacatc
    12061 agcataaatc cccggtcgcg gtatttaggc gtcagcgcca tgataattcc tatttctttc
    12121 acaccgggcc cgggcaatga accgcaaaat aagatacccc aataaccggt ctttttaata
    12181 aaccgtttga aacctgatgt ttcatcaggc gtttcagcgg tttgatggtc tatttctttt
    12241 tttacaaaaa agccgcgaaa tcggggaaac catttagttt cttccaaacg ggcgattatg
    12301 cggtgtgacc aggctgtttg tccgatacgc tgaaatgatg tacgaaataa aaagaacatg
    12361 attttgagcc aaaaaaaatc aatcacggag acgatgacca gtgtggccgc cgctgcgttt
    12421 gtccagtgtc cgaagtcaga ttctaaagca cgaaaccaaa aaataacccc ggcttcgtgc
    12481 caaaaaatcg tcgtaaaaat cagcggaaaa aaagtatata tcgtaatatc agtcaatgaa
    12541 tatgaaccgt atcaagattg gcgcttgaat ttattgaaat attacgacaa tttagtttga
    12601 tgtccaacag aaaacgagga gcttgtgaag acgttattga tggccgtgtg tttttttgta
    12661 cttcaggatc agcctgcatt tgatccatcg ctcatgtatg ggcgctggaa gtggtacgcg
    12721 agtgagggag ggctgatgga acagcgcatt acggtcgaac aaactcaaca aaacaaatcg
    12781 ctgctcttta caaaagatca tgccgttttt tatatggttg gggattctgt aagctacaaa
    12841 agccgctatg ttaccgaaat gcaccgctca gaatttaccg gtcaagacac gccgctgtta
    12901 cgcatacaag gtatttccaa agatcaatgg ataacgatgc aaggaaaaga ttcgctgatt
    12961 ctacgtgaaa ataaatacga cggatggacg catcgttatg tgcgtcttca ttagatgcgc
    13021 ctaaccaagt cttgatttta ctcggctttt gatgtaactt gccgcatgat ttagcaataa
    13081 tgtaatgcat tttagccagc cctaaacgaa aggctggttt ttttattatc ggtacagcgt
    13141 ggcagaaaag aaaaatacca atcggtttga taatctggaa atccagcctc ggtcgcattc
    13201 ggaaaccgta gcacaccccg ccccgggttc tactaaaaac gaacgtttcg agtggatcga
    13261 agttcctcaa cagaaaacat ttaccaaacg ttttaatact aaagcccgta gtatcacatc
    13321 caaacggtgt ccacgctgcg gatggctgga tcaatcggcc gtgaccgaat gttttcgttg
    13381 cggttatgat ttccaaacca acgacatgca tcttgatttt tttaagcagg aaaagattga
    13441 cctgcctccg gtagaaatca aagtggatat gggttttcag gatgttctgt cgaaaaagca
    13501 atatgataca cttgatgctt atcaattaag actcgaagcg gaacgtctta acctgatttc
    13561 aggttttgac cgtcttatcg ccgtaaacga tctgaatatc acacactacg attaccagat
    13621 caataccgct cttgaagcct tgcgacgcat gcgcgggcaa gttcttttag ccgatgaagt
    13681 ggggcttggt aaaaccatcg aagccggaat catcaccaaa gaactgattg agcgtaattt
    13741 agctaaaaat attctgatct taacaccggc atcattggtt ggacaatggc aagacgaatt
    13801 actcatgaag tttggtgaga attttatcgt accgcaacag gagagcgatt ggcgtgcgcc
    13861 gaaagtcatt gcttcgattg atatggccaa acgtaacgaa gcggccaata ttttgctgtc
    13921 gagagatttt gatttattga tcgtagatga agcgcaccga ctcaaacatc gttcgacgat
    13981 cggtttcaaa tttgtgaaca aaatccgtaa aaaatatgtg cttttgttga cggcaacacc
    14041 tgttcacaac gatctaaccg aattatacag ccttgtaacc atactcaaac cgggcttatt
    14101 cgggaccatt cgctctttca aacgaaaatt tatgttgaag gatgatccgc gccttcccaa
    14161 taacgaggat caacttaaac acatccttca ggacgtaatg atccgcaacc gccgcgataa
    14221 agtcggcatc aatctaccgc cgcgtaaagc cgcgatttat catctggatc tcacgccgat
    14281 cgaaagtgaa ctctatcgtg aagtgacgaa ttatgtgcgc gaggaatttc gccgcgaggc
    14341 gaattttgac gtacatatcc tctcgctgat cacgctgcag cgtgaggtct gcagtagccc
    14401 gcaagccaca cggcatacat tgattaattt tatgaatcgt aattacccgg cgaaaacccg
    14461 tgaacgcctg gcgtatttcg tagagctttg tgatgccgtc aaaatcagcc gcaaaagcgc
    14521 ggccaccatc gaattgctaa aaaaatttcc ctccaaaacc ataatttttg tggaattcat
    14581 tgagacgatg aaacatctgc aaatggagct agagcaggaa ggtatttctg ttgagctgtt
    14641 tcacggagga ctgtcgggga cgcatgcgcg taaggaggcg attgataggt ttcgcaaatc
    14701 caaacaggtg ctcatcagta cgcaagccgg cggggaaggg ctcaatcttc agttttgcag
    14761 tcagatcatc aactatgact taccatggaa tccgatgcgt gtcgaacaac gcattgggcg
    14821 cgttcatcgt ctgggtcagg aacgcgacgt ttttgttttt aacctttccg ttaccgacac
    14881 gatcgaatcc cgtatattgg aacttttggc taataaaatt cgtatgtttg aactcgtgat
    14941 cggcgaactg gatatcatac tcggcgatat cgaaaccgaa aagaattttg agcaacagtt
    15001 gatggaaatt tttatgcgca gcaccgatga aaaagaaatg aatcaacagt tagaacgttt
    15061 cggtaatcaa cttacgcatg ttcgccgtca gtatgataaa gtacgtgaaa attcagaaat
    15121 cttatccgat attgtagacg tttaactcat caaaaggagt aatcccgatt cgtcattgtt
    15181 accgctcttg ttttttttaa caaacaccga ggtcaatatg caaaaaaaac gtatcatctg
    15241 ggcggatgac gaaatcgaat tactcaaacc ccacatttta tttctcgaac aacgcggtta
    15301 cgaagtaacg cccgtcacta acggcgaaga tgcgatcagt caggttcgca cccggaagta
    15361 tgaccttgta ttgctggatg agatgatgga cggtaaagat ggtttgacga cgctcgaaga
    15421 gataaaagaa aacagcccca ctattccagt cgtgatgatt accaaaagtg aagaagaacg
    15481 cctgatggaa gatgccatcg gacggaagat tgatttttat cttaccaaac cggtcaaccc
    15541 cagtcagata ctgatggcat gcaaacagat tttcgaatcg cgcaaattga cggaacaaaa
    15601 aatttcgcgt gattatgtac aggagtttaa tcgcatttca cagaaattga tggatcggct
    15661 ggaatttaag gattggtatg atatcgcgca atccatggcc gagtgggagg ttgagctgga
    15721 tgcgctgacc gataccgggc ttcggcagat gcttgaggat cagcgcaaag cttgtaatgt
    15781 cgaatttggt aagtttgtcg aaaaaaacta tcacgggtgg gtgaactcag ggctgcctca
    15841 acgaccggaa ttttcagtgg atattgttcg taactacgta tttccgaaaa tcgcggcacg
    15901 tcgtaaagtc gtatttattg tgatagactg tctgcgcatt gatcagtggc taggatttga
    15961 atcggtgttg caggagtatt ttaatatcaa taagaagttc tattatagca ttctcccgac
    16021 atcaacgccg tattcgcgta atgcgatttt tagcggaatg tggcccagtg agattgagca
    16081 aaaatttccc gatatatgga gtaaaggcga agaagacgac tccagtcgca atcggtttga
    16141 aaaagaactt ttggaatacc aattgaaatc caatcgtgta ccgatggaaa cagaggctaa
    16201 gtatgtcaaa atacttaacg ccgaacattc acggcaattg gagaccaata ttaccagttt
    16261 tgtcaatagt tcacaactta cggcgatcgt tctcaatttt ctggatatcc tcgcacatac
    16321 gcgcagcgat tcgcatgtga tcaaagagat cgcaccgcac gaaccggcgt accgctcgct
    16381 gagcaattca tggtttacgc attcttcgtt tttagaaata ttaaaaacac tttccagact
    16441 ggactgtcag atcgtcatca catccgatca cggcagtatt cgcagtttgc atggtactga
    16501 agttatcggc gataaagaga cgtcaacaag tttgcgctat aaatacggaa agaacatcaa
    16561 gtccaatccc aaacattcga tgtttatcaa aaatccgctg gagtataaac tgcccaaacg
    16621 cggggtcaac acgcactata tcatcgccaa agaagattat tactttttgt atccgacgaa
    16681 ttaccatcac tttctgaatc actataatga tacattccaa catggcggga tttcggtcga
    16741 agaggtgatt atgccggtga tcgagatgga aggaaagcga taaacagtaa cctatatttt
    16801 attatttgag gaataggttt aattgcaaat aattaaacct attcctcttt tttgtgcgaa
    16861 atagttattt ggtcatagtg gtacgtatcg ccccccgatg caggcgttcg atatccgatg
    16921 cttgcatgac gatccagtca ttgatccgta aagttttacc gccaacgact ccgatctcca
    16981 cacaatccac atgctgagtt tgcgcccatt gtttcaatcg ttctgcattt tccgtatcgg
    17041 ttgttaatat gatgcgcgat tgcgtttcgc taaacagcag gaaatcaggc cgtaatgcat
    17101 cgttaagacg gattgtagcg ccgatggtgt ggccgggttt accttgataa caagattcgg
    17161 ctaaagctac ggcaagtccg ccatcggata catcgtgtgc cgaacgaacc cagcctttga
    17221 tgatcgtatc caatacaaaa tcctgaactt tcttttcgta cgccatatcg agcgatggtg
    17281 catcgccgct gattttaccg tgaatcgttt tcaggtattc gctaccgccg atatccgttc
    17341 cgttttttcc gataaggaat atcttgtcgc cttcggattt gaaccatgat gtcgtgacat
    17401 gtttgaggtc ttcgataatt ccgatcatac cgatagtcgg cgtagggaat acggcattgt
    17461 tccggctttc attgtaaaag ctcacattcc
//
LOCUS       DAOQGF010000069        17361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1511_length_17361_cov_7.088755, whole genome shotgun sequence.
ACCESSION   DAOQGF010000069 DAOQGF010000000
VERSION     DAOQGF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1511_length_17361_cov_7.088755"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..597)
                     /locus_tag="PK937_10230"
     CDS             complement(184..597)
                     /locus_tag="PK937_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32991.1"
                     /translation="MKFTSYSIWVAVFILVSDGCISKTMISRRSELKQAVAEQNKLVV
                     QLKNDRLLVLEEVTYSDTAIYAKGYEIKGDRHIPVSESIVLDDVIYAEYNKRSILGGL
                     VGAGIVGVSAIAAVAIAKDPGKFRMNRGYACGADE"
     gene            complement(599..1315)
                     /locus_tag="PK937_10235"
     CDS             complement(599..1315)
                     /locus_tag="PK937_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH32992.1"
                     /translation="MFKILTFTTVILSASLLAQDLSENTPSQGVLRKTMYQRSKTQKD
                     SLRVTRDPARLHVQPYPNFGIGMGGSGYWMLHDKFQHVYKDVGEPTTSPLLWLTMELQ
                     LSKELTIHQNTTLGLAEMGLHTVSASLCYRFLHSGTFQPFIGGGAGIYIFDLYKERGD
                     YLASGHGKKWGLQYVAGIDIGPYNRDTGLTLFAQYADVRPFRGRLHDDVESQYDNTPI
                     TVDISGLMIGARLTFYFGGY"
     gene            1746..2999
                     /locus_tag="PK937_10240"
     CDS             1746..2999
                     /locus_tag="PK937_10240"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNH32993.1"
                     /translation="MDRKIEKKTWTPKRIAIIGGGALFVFFIAYQFLFGDNSTKLNVK
                     SDKITIAKVSKGPFQEFIPVTGNVMPIKTIYLDAMEGGRVDKLFVEEGAMLKQGEEIL
                     KLTNTNLQMDVMFREAQLFEQINNLRNTRLSYEQTRLRNKSDQINLDYEIQKLKRTYD
                     IQKGLQSQNLTTPQEFETARDEYDYAAKRRDLLLETMRQDSILRAVQMQSLEASIEHM
                     GTNLNTIKENLANLTIRAPIAGQLTSRNAELGESKRSGDRLGQIDVLDGFKVRAGIDE
                     HYIARIQIGQTGEFDLAGKTYNLVIKKVYPEVQNGRFEVDMEFQGDTPKDIRRGQSLQ
                     IRLELGDLQEAVLVPRGGFYQKTGGQWIFVVDPSGDFAVKRNIRLGRQNPQVFEVMDG
                     IFPGEQVITSSYDSYGDVDKLMLNK"
     gene            3078..3470
                     /locus_tag="PK937_10245"
     CDS             3078..3470
                     /locus_tag="PK937_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNH32994.1"
                     /translation="MNENGMLNLGHKNLDVWKLSIELVTKIYAITENFPKTEMYGITN
                     QLRRAAVSVSSNIAEGAARRSQLERRRYYEIARSSLVEIDTQLEIALRLKFCIRENLH
                     SISDLLNHVFASLSKLVKSTIHKTLTKN"
     gene            3498..4187
                     /locus_tag="PK937_10250"
     CDS             3498..4187
                     /locus_tag="PK937_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH32995.1"
                     /translation="MIKAKNLNKMYTTDEVETTALNNVNFEINDSEFVAIMGPSGCGK
                     STLLNIIGLLDNPTSGEYHFVGHEVSRYSERQRSNLRKANIGFVFQSFNLIDELTVYE
                     NVELPLLYLGMTASDRKKRVEETLEHMQIMHRRNHFPQQLSGGQQQRVAVSRAVVAKP
                     KLILADEPTGNLDSAHGEEVMRLLTDLNKAGTTIIMVTHSPSHAEYAHRIIHLFDGHI
                     VTENMKEAAKI"
     gene            4241..6613
                     /locus_tag="PK937_10255"
     CDS             4241..6613
                     /locus_tag="PK937_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32996.1"
                     /translation="MIRNYLKIAVRNMQKNALHTFFNVLGLAMGIACCITVYIFVDTM
                     LNMDTAHREAERIFTINHVRLINNREEFWGLSKGPLGPAIKNDFPEVTDFIRLDDNNA
                     VVKRGDLIFNEDIRFADANFFSMFTFSLASGVGSDLAQKNAIFLSEEKALKYFGDQNP
                     VGQTLTLIFEGEKRLEFVVKGVAKKFPRNTSFAFNFLVNYENIKDIYPQADRWTYNVS
                     ATFIKLHRPEDAFKVEQGLSRYLALQNESNPNQPAQRFYLDNLLTMSKNSWQVRGDIS
                     GGTNPHGIYTLTFIAALVMLMACFNYINIAVASAARRFKEIGIRKVVGGTRGQLVKQF
                     LTENFITTLTALGLGVVFAETLTIPFFNSIIQGNTVLAFDISADLRVVIFLAVLTSSI
                     GLLAGLYPSLYMSSFKPVNILKDVQKIDGNRWLGKTLLVLQFSISLFAVIAGIIFTQN
                     AEYIKTLDYGYEDDQIVALYFKQSAQFHQFEQKIRQDSRIHAIAGSAHHAGRNIMGNV
                     VYESNGAPSQANVMRVGSDYIQTLALRMKQGRAFDRAMTTDAENSAIVNATLVKAQGW
                     TEPIGQRLKMNDKELTVVGVVEDFFYRSYHRAIDPCILTMIPDDEYRYMSIKVAPEHV
                     LSIRPLLESTWREIMPDEPFDMIYQNEIFLEDLTEDEQVKNVFLYVAIMTLMIAAMGL
                     FALVSLSIAKRTKEIGIRKVLGASVAHVIHLVNRDFYWLIGIASVIIFPIAFFLLTNL
                     LNSIYAVHVDITALPFILSVLIFGLVAIITIGMRVYGVAKENPVHALKYE"
     gene            6693..9095
                     /locus_tag="PK937_10260"
     CDS             6693..9095
                     /locus_tag="PK937_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32997.1"
                     /translation="MIKNYLQTAWRVLLRNRGYASINIFGLTLGMTCAFMIFLIVRFH
                     LSFNDQHRFKDRLYRVTTTDKRAAQEDETSMGTPYPLSGLLRTGFPEIENVAVTDYVR
                     NGQVLISQQNGEPKKFSETGGIAFVQPQFFDMFDFPWIEGTPKTLDELNTVALSQTLS
                     QKYFPEGNPIGKIIRLDNEHDLKVAGIFKDFPANSDFPFQMAIAFETSRAMYPNNKTE
                     WGSTSSNTNTYVLFKENFDLRAAEAKLNEEAKKHREIRPGFDRIFLFQPLDAIHYDSN
                     FGSYAGATISQNTIITLGLIGFFLIVTACINFVNLATAQAVKRAKEVGVRKVLGANRF
                     QLVAQFYGETILITLFAVILTFGLTEFSFPKVMEWLDLTIPSVLIWNADSLLFIAALS
                     VMVIFLAGLYPALVLSGFGPALALKSKITSYHSGGLYLRKGLVVLQFSISQLLIIGTI
                     IATTQLDFFNKQDLGFNKEAVVIVPIHERTKTLLDNLEHQWKSHPGVESVSFAYASAA
                     SGNRWTSNVTFLKKDEPIELDVDLKMADDHYADIFGLQFLAGRNYVAGDTLREVVINE
                     TMVRKLGFSNPQDAIGIELKIGARRRVSVVGAVRDFNASSLHDPIAPCIIATNSRFYQ
                     EASIKINMQQATDVLRHIETVWNTAFPQYVFDRRFQDETIAEFYEQEQIYTNMLRIFS
                     GIAIFIGCLGLFGLVSFMAAQKTKEIGVRKVLGASVTDILGMFAKEFIALIILAFIIA
                     APLAYYAMQQWLQDFEYRITLGPAVFAAAVMVSLIIAGATVGYQVFRAARANPADALK
                     YE"
     gene            9128..11497
                     /locus_tag="PK937_10265"
     CDS             9128..11497
                     /locus_tag="PK937_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32998.1"
                     /translation="MIRNYFIIAFRNLYRKKMYTFINLFGLSVSIAVCIVGYLIWDYS
                     YHFDQFHENAERIYHIGTTRVINEQDQIFGVSPLPLAPALARDFSGVKRAVRFANQVA
                     IVRVGDKVFNESIHFADEQFFEMFRFELEHGRYDALKSKTDLIITHDMAMKYFGEANV
                     VGQSMTISYANEVKQTYTIGGVLKTLPRNSSLRFGFLLSSEILSDLGVDERNNWSHVT
                     GATFIEVHQVETIQQIKGSESKYIELVRSANPRFPISHLVITPLKDLALEGESFRSHD
                     LQDAPPPSARISLFVTSILLLVMSCFNYMNTSLAMATARLKEIGIRKVMGSPRTQLIF
                     QFLTENVLLCVLAIFAAFGLAEFLVPGWNALFYDIQLSMDESKSPGLWLFLIGLVGLT
                     AVGGGLYPAYVISAHNPAKILQGHQSKTTASWIMKVMLTLQFSVSIIAVVGSVVFSQN
                     AEYQKNLDLGYEPEKLIGVVIQKSSMYPVLSNALMQNSQIESVSAGRNHILYSSWRRS
                     IKVVDRDIEAEFLYVRPGYTETVGLKLAEGRFFDVSQPLDLTESIVINRMMAKEQGWE
                     NPIGQTMVIDSVTCRVVGVIEDFYIRGPFRPILPCVFKAANEDSYRFVIVKADHNNIH
                     AAMRSVEETWKKLFPEMPFDGFYQETQTANAAELSKSIMLVFFCVALMAIIISGMGLF
                     ALVSLNIVRRTKEIGIRKVLGASALQIMRMMNSDFIILLLIASVIADVAGFFLFKALL
                     GSIYRYHVEVGIMALILANIAVLLIGLATIGGRVLKTARANPVNALKYE"
     gene            11527..13941
                     /locus_tag="PK937_10270"
     CDS             11527..13941
                     /locus_tag="PK937_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH32999.1"
                     /translation="MSRNYLLIAIRNLFKQKGFALINTSGLAVGIAGCLLILLFVRDE
                     LSYDQYHSKARRIFRLQTNVDVSGNLLTLATTSYPMAEALKTDHSQVESAIRVMRWND
                     PILSQGDIRLKETSMLWADAGVFDVFSWPMIYGDPKTALQEINSVVLSESASKKYFGD
                     DNPIGKILRYDGRIDLLVKGVIKDIPYNSHVRFEVLANIQTLLDVVGRESMSHWHAFY
                     QLQTYLLLRNTFDAQSLIESLPSFVDKYLKGEFAESVGRTYTMELTPLTELHLSTSRK
                     GEFSTVGSMNYVYVFSGIAFCLLSIACINFINLSTAHATKRHREIGLRKVLGANRSQL
                     IRQFLTETTLLVFISITVALLLIDIVLPYFNTLAGKHFTFSIFADYTTLLTIPGLVVI
                     VGILAGMYPAFYLSRFNPIESVRGSHYSGTKKTRFRHALVSIQFVVSIVLLIITAVVF
                     RQLDFIQNKNIGIDKNQTLVIPVTDQQIRARYHSLREQLTTQPGIENVAISGFMPGIR
                     VPGSPLQKIPTGPSDKWEFSTIPADENFVPTLGINIIAGRNFTKLNPTDSAESMLINE
                     AAARDLGWTPEEAIGKPLAWGGDPGFNVRVIGVIKDFNYTSLHQPIKPLVIIPTALWP
                     NSYNFLSIRINTGHLSETIHHINTVWEQRMPETPFIYSFLDDDFSKLYAGENKLGIIF
                     LIFASLAIFVASLGLLGLATFVSESRRKEISIRKVVGADLRTLIILLLGDFLMPITIS
                     TLISWPIAYYIASAWLQNFAYRTEPTWYVFVAATLFSIGIAALMLIQYVIKTARVNPA
                     VTLKSE"
     gene            14055..16448
                     /locus_tag="PK937_10275"
     CDS             14055..16448
                     /locus_tag="PK937_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33000.1"
                     /translation="MVKNFLIVAMRTMRKHKSYTIMNVTGLATGLACFMVIAMYVRRE
                     TAFDTFHKNPDRLFRVALYRENTGSPQPIATVSHSVADILRRESPGIESMARLFRFDQ
                     DAVILAGEKKFTEPDLMFAEPDVFDLLSIELLNGSPANALKNPTDAVLSEASARAYFG
                     HTDVIGQSIRVRINTRYADFKITGVFKNYPSNSHFYTNVLLGFDGVKNYVGDVDFFQS
                     WFNYPMWTYLRIKDIQDEVSVNAALHQIVERHFPQTRKPAHMQLQRITDIHLHSQMSG
                     EIRPNSNIFYIYTFTVIGLLLLIVACVNYINLSTAQSEQRTREVGVRKVMGARRRELI
                     FQFLMESVLQSMLALGLALGLAEVLASVLNRFTDLQLTFDYSDPFLFIFSAFLIVVVG
                     VGAGFYPAVVLSGYAPIRNLRMKAFRRRFPLRKILVIVQLSVTAALISAVWIIQLQIN
                     YVRSKELGFNKDHTVLIRAIGSTVASNLNYDRFKYQCEQLPGVISVTRHSWVAGEGFR
                     IRSVGFDSFSEEQRQPIQFIFVGEDYLKTYDLKLIEGRDFSAQYPADTAGAYIVNEAA
                     VKAYGLTNPIGRIAVSGDRGPVRGPIVGVVKDFHFASLHETIEPMVIGLFRFPMPYIS
                     VRFDGQATPETIGAIERLWNNIDTERPMDYSFLDTRIERVYRFENQLSAITKLFTILS
                     MIIGCMGLFGLIAAMAEKRTKEIGIRKVLGASIWGIIGMFAREFSAMVIVATLLAVPT
                     VYSGLSQWLEGFAYRVELTAEPFIVSFIAILFLTVITVAHHALKAAKTNPAVSLKSE"
     gene            16537..>17361
                     /locus_tag="PK937_10280"
     CDS             16537..>17361
                     /locus_tag="PK937_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33001.1"
                     /translation="MFKVYLKIILRNFLKERSFVWINLIGLSVGLLCCFTILLYARKE
                     LAYENFHVNKDHIYRVLPRIQGGANDYEQIWTPAWLAPSAKNKIPEIRLASRYIHLGS
                     EQLITHENQRMLTDGLALADPDFLHMFSFTEPFTSDMNPLSDPSALIVSRSAAYRIFG
                     DQSPIGKTITLNGSHVFTIKAVFTDVPVESHLQFEMLGNFQRIREIWGMNTTPEENTS
                     WNFATYLMTTPQADISAVQSKVLNIFEERAPRKNQRTVRLQSLNDIHFTQGIRRDNA"
BASE COUNT         4694 a         4106 c         3823 g         4738 t
ORIGIN      
        1 tagcgagtac ctattccctc gcgcaaagcc gcaaagagaa atgaaaaaag tcttttgata
       61 taatttttta tagatcgcct atttgtcaac gaaccattca ccggtcgcta tacccatgcc
      121 aattcaatta caaaatcgtt attcctatcc cgggatgttt atcgcgcttc acgtcgacat
      181 tatttactca tctgcaccgc acgcataacc gcggttcatc ctgaattttc cgggatcttt
      241 ggcgatggca acggcggcga tcgctgaaac gcctacaata cccgcaccga caaggcctcc
      301 caggatactg cgcttattgt actccgcata gatcacatca tcgagtacga tggattcact
      361 gacaggaata tgcctgtcgc ctttgatttc atatcctttg gcatatatcg ccgtatcgga
      421 atacgtcacc tcctccaaaa ccaacaaacg gtcgtttttt agttgtacga ccaatttatt
      481 ttgttcagcc accgcttgtt tcagttcaga acggcgggaa atcatggtct tgctgatgca
      541 accgtccgag acgagtatga ataccgcgac ccagatcgag tacgatgtga atttcatatc
      601 aatatcctcc aaaataaaat gttaatcggg caccgatcat cagaccgctg atatctacag
      661 tgatcggcgt attgtcatat tgcgattcga cgtcatcatg gagtcggccg cgaaatggcc
      721 ggacgtcggc gtattgtgca aataacgtaa gccccgtatc gcgattgtac ggtccgatgt
      781 caataccagc gacatattgc aagccccact tttttccgtg accggacgcg aggtaatcac
      841 ctcgctcttt atacaaatca aaaatataaa tacctgcgcc gccgccgata aaaggttgga
      901 atgtacctga atgaagaaaa cgataacaca acgaagcgga tacggtgtgc aatcccattt
      961 cggctaatcc cagtgtcgta ttttgatgaa tcgtcaattc ctttgacaat tggagttcca
     1021 tggtcagcca taaaagcggt gacgtcgtcg gttcgcccac atctttatat acgtgctgaa
     1081 atttatcatg caacatccag taccccgaac cgcccatgcc gattccaaaa ttggggtatg
     1141 gctgaacatg cagacgggca ggatcacgcg tgacacggag cgaatccttt tgagttttcg
     1201 aacgttggta catcgttttt cttaggacgc cttgcgatgg cgtgttttct gaaaggtcct
     1261 gcgccaaaag tgatgcgctc agaatgacgg tcgtgaaagt tagtattttg aacatgatcg
     1321 ttgtccctcg aagaatgaat cactacgacg ttttaataac atatctgatc atgtcaaaca
     1381 ccgtgccgaa aagcgagcgc agttttttat agagttgtaa aaagctcccg aagaaacccg
     1441 cgatttaaaa aaacacactg ccataaaaaa atggcgtgtg cttaggatgc aaaaaaattg
     1501 aaatgatttt ttctatgaac caacgattga atcattttag caaaacctcc gatacctgaa
     1561 tttggaacct atttatagag tgtacggatt cgtacacccc tgcacgcata cgaacgattt
     1621 gtgtgctcat ttataaattg aaaatcatta aaaacaatta tttaaccatt ggcataagaa
     1681 ttgacaataa atagtattga gttaggttta tcaattatca accgcattga aacacgacag
     1741 gtaacatgga ccggaaaatc gaaaaaaaga catggactcc caaacgcatt gcgatcatcg
     1801 gtggtggtgc gttgtttgtt tttttcatcg cttatcaatt tttatttggt gacaattcca
     1861 cgaagctcaa tgtcaaatcc gacaaaatca ccattgccaa agtttccaaa ggcccgtttc
     1921 aggaattcat acctgtaacc ggcaacgtca tgccgatcaa aacgatttac ctcgacgcga
     1981 tggaaggcgg acgtgtggac aaacttttcg tcgaagaagg cgcgatgctc aaacaaggcg
     2041 aagagattct caaactgacg aacaccaatt tgcaaatgga tgtcatgttt cgcgaagcgc
     2101 agttatttga acaaatcaac aatctgcgca atacgcggtt atcttacgaa caaacccgtt
     2161 tgcgcaacaa aagcgaccag atcaatctgg attatgaaat tcagaaactc aaacgcacgt
     2221 atgatattca gaaaggcctg caatcgcaga atctgaccac gccgcaagaa tttgaaaccg
     2281 cgcgggacga atacgactac gccgccaagc ggcgcgatct gctattggaa accatgcgcc
     2341 aggattcgat cttgcgcgcc gtgcagatgc aatcgctgga agccagtatc gagcacatgg
     2401 gaaccaacct caataccatc aaagaaaacc tcgccaatct taccatccgc gcgcctatcg
     2461 cagggcaact cacgtcacgc aacgcggaac ttggcgaatc caaacgctcc ggcgatcgcc
     2521 tcgggcaaat cgacgtgctg gacggcttta aagtgcgtgc cggcattgac gaacactaca
     2581 tcgcacgaat tcagatcggg cagaccggcg aatttgatct cgccggaaaa acgtacaatc
     2641 tcgtgatcaa aaaagtttat ccggaagtcc agaacggacg ttttgaagtg gatatggaat
     2701 ttcaaggcga cacacctaag gacattcgtc gcggacaaag tttacagatc cgtctggaat
     2761 tgggcgattt gcaggaagcc gtacttgtac cccgcggcgg attttatcag aaaaccggcg
     2821 ggcaatggat ttttgtcgtc gatccgtccg gcgactttgc cgtaaaacgg aatattcgcc
     2881 tcgggcgcca aaacccgcaa gtttttgaag tcatggacgg aatcttcccc ggcgaacaag
     2941 tcatcacatc cagctacgac agctacggtg atgtggacaa actcatgctg aataagtagc
     3001 agacggtaga cggcagacgg gaaacgggac acgggaaacg gcagacggaa agcgataata
     3061 aattaggaga ttttgtaatg aatgagaacg gtatgctgaa tttgggtcat aaaaatttag
     3121 atgtatggaa actgagtatc gaattggtaa ctaaaattta cgccataaca gaaaattttc
     3181 caaaaacaga gatgtatggt atcaccaatc aacttcggcg agcggcggtg tcggtttcat
     3241 caaatatcgc cgaaggcgct gcacgacgat ctcaactcga acgacgtcgc tactatgaga
     3301 ttgcgcgttc ttctttagta gaaattgata cgcaattaga aattgcgtta cgcctgaagt
     3361 tttgcatcag agaaaatttg cattctatct ctgatctact aaaccatgta tttgccagtc
     3421 tttctaaatt agtaaaaagc actatccata aaacattaac gaaaaattaa tccatatcac
     3481 ttttaggaga tgcctgtatg atcaaagcca agaacctaaa caagatgtac accacggacg
     3541 aagtggaaac aaccgcactg aataacgtca actttgaaat caacgacagc gaattcgtcg
     3601 cgatcatggg accatccggt tgcggtaaat ccacgctgct caatatcatc ggcttactcg
     3661 acaatccgac cagcggtgaa tatcattttg tcgggcatga ggtatcgcgc tactccgagc
     3721 gccaacgttc caatctgcgc aaagccaata tcggttttgt gtttcagagt tttaacctca
     3781 tcgacgaact gacggtgtat gaaaatgtcg agttgccgct tttgtatctc ggtatgacgg
     3841 catcggatcg taaaaaacgc gtcgaagaaa cgctggaaca tatgcaaatc atgcaccgcc
     3901 gcaatcactt tccgcaacaa ctttccggcg gtcagcagca gcgtgtggcc gtgtcgcgcg
     3961 ccgtcgtcgc caaacccaaa ctgatcctgg ccgacgaacc tacgggtaac cttgattccg
     4021 cgcacggtga agaagtgatg cgtctgctga ccgacctcaa caaagccggt accaccatca
     4081 tcatggtaac gcactccccg tcgcatgccg aatatgcgca ccgcatcatt cacctcttcg
     4141 acgggcacat cgtgacggag aacatgaaag aagcagccaa gatctgacca cgaacggatg
     4201 cacacgacga gaaacaaagt aaaactataa ggcggggact atgatcagga attatctgaa
     4261 aatcgcggta cggaatatgc aaaaaaatgc tttgcatact ttttttaacg tattgggact
     4321 ggcgatgggt atcgcatgct gtatcacggt atatatattc gtggatacga tgctcaatat
     4381 ggataccgcg catcgcgagg ccgaacgaat attcacgatc aatcacgttc gcctgatcaa
     4441 taaccgcgaa gaattctggg gattgtccaa aggaccgctt ggcccggcta ttaaaaatga
     4501 ctttccggaa gtcactgatt ttatccgtct ggatgacaat aatgccgtgg tcaagcgcgg
     4561 cgatcttatc tttaacgaag atattcgttt tgctgacgcc aatttctttt ccatgttcac
     4621 cttttcgttg gcttccggcg tcggcagcga tctggctcag aaaaacgcca tttttttgag
     4681 cgaagaaaaa gcgctgaaat attttggcga tcaaaatcct gtcggtcaaa cgttgacttt
     4741 gatttttgaa ggcgaaaaac gtttggagtt tgtcgtcaaa ggtgtggcta aaaaatttcc
     4801 ccgcaacacc agtttcgcat tcaactttct cgtcaattat gaaaacatca aagacattta
     4861 cccccaagcg gatcgctgga cttacaacgt cagcgctaca tttatcaagc tgcatcgtcc
     4921 ggaagatgcg ttcaaagtag aacaggggct ttcccgatat ttggcgcttc aaaatgaaag
     4981 caaccccaat cagcccgcgc agcgttttta tctcgacaat ctgctcacga tgtctaaaaa
     5041 ttcgtggcag gtacgcggtg atatttccgg cggcaccaat ccgcacggca tttacacact
     5101 gactttcatc gccgcgctgg tcatgctgat ggcctgcttt aactatatca atatagccgt
     5161 ggcctcggcg gcacgtcgtt ttaaagaaat cggaattcgc aaagtcgtcg gcggaacccg
     5221 cgggcaactc gtcaaacaat tcctgacaga aaattttatt accacgctga cggctttagg
     5281 tttaggtgtc gtgtttgcag aaactcttac cataccattt tttaattcca tcatacaagg
     5341 caacacggta ttagcgtttg atatttccgc tgatctgcgc gtagttattt ttttagccgt
     5401 actgacttct tcgatcggac ttttagcagg gctgtatccg tcgttgtaca tgagttcgtt
     5461 caaacctgtt aacattctca aagatgttca aaaaatagac ggcaaccgtt ggttgggtaa
     5521 aacgcttctc gtattacaat tttccatttc actttttgcg gtcatcgccg gcattatttt
     5581 tacacaaaac gccgaataca tcaaaacttt ggattacggc tacgaagacg atcagatcgt
     5641 cgctttgtat ttcaaacaat ccgcgcagtt tcatcaattt gaacaaaaaa tacggcagga
     5701 ctcgagaatt cacgccatcg ccggttccgc gcatcacgcc ggacgcaata tcatgggaaa
     5761 tgtcgtttat gaatccaacg gtgcgccatc ccaagctaac gtcatgcgcg tgggttccga
     5821 ctacatacag actttggctt tgcgcatgaa acaaggacgc gcttttgatc gtgcgatgac
     5881 gaccgacgcc gaaaatagcg ccatcgtcaa tgcgaccttg gtcaaggcac aaggatggac
     5941 ggaacccata ggacaacgtc tcaaaatgaa cgacaaagaa ttgactgtcg tcggtgtcgt
     6001 ggaagatttc ttttaccgtt cgtatcaccg cgccatagat ccttgtatcc taacgatgat
     6061 accggatgac gaataccgct acatgtccat caaagtagcg ccggaacacg ttttatccat
     6121 tcgtccgtta ttggaatcaa catggcgcga aatcatgccg gacgaaccgt ttgacatgat
     6181 ttatcaaaat gaaatttttc tggaagatct gacggaagac gagcaagtaa aaaacgtatt
     6241 tctgtatgtt gccatcatga ccttaatgat cgccgcgatg gggctatttg ctttggtatc
     6301 gctatctatc gccaaacgga cgaaagaaat cggaatacgc aaagtattag gtgcatccgt
     6361 agcacacgtg atacaccttg tcaatcggga cttctattgg ctgatcggta ttgcttcggt
     6421 gattattttt ccgattgctt tttttctgct taccaatcta ctcaacagca tctacgccgt
     6481 acatgtggat atcacggcat tacccttcat actcagcgta ttgatattcg gcctcgttgc
     6541 catcatcaca atcggcatgc gggtatatgg cgtcgcgaag gaaaaccccg tacatgcttt
     6601 gaagtatgaa taaccgacgg aaaactacat cgtaacaagt cattctgttt gacgatgttt
     6661 gattttgaaa atttttattt cggagtacct atatgatcaa aaattattta caaaccgcat
     6721 ggcgggtttt gctgcgcaat cgcggatacg cgtctattaa tattttcggt ctgacacttg
     6781 gtatgacctg cgcattcatg atctttctga tcgtacgctt tcacctcagt ttcaacgacc
     6841 agcatcgttt caaagatcgc ctttatcgcg tcacgacgac ggataaacgc gccgctcagg
     6901 aagacgaaac gagcatgggc accccttacc ctttatcggg tttactgcgc accggttttc
     6961 cggaaatcga aaatgtggcc gtaaccgact acgtacgcaa cggtcaggtt ctgatatcgc
     7021 aacagaacgg cgagcccaaa aaattcagcg aaaccggcgg catcgcattt gttcagcctc
     7081 agttttttga catgtttgat tttccctgga tcgagggcac gcccaaaact ctggacgaac
     7141 tgaataccgt tgctttgagt caaacgttga gtcagaaata tttccccgaa ggtaatccca
     7201 tcggaaaaat cattcgctta gataatgagc acgatctcaa agttgcgggc attttcaaag
     7261 attttccggc taactccgac ttcccatttc agatggcgat cgcgtttgaa acctcgcggg
     7321 ccatgtaccc caacaacaaa acagaatggg gttctacctc gagcaatacg aacacatacg
     7381 tactatttaa agaaaatttt gaccttcgcg cggctgaggc caaacttaac gaagaagcca
     7441 aaaaacaccg ggagatacgc ccgggttttg atcggatttt tttatttcag ccgcttgacg
     7501 ccattcatta cgacagtaat ttcggttcgt atgccggcgc caccataagt caaaacacga
     7561 ttatcacact cggtttgatc ggatttttcc tgatagtaac cgcgtgcatt aatttcgtta
     7621 acctcgcaac ggcgcaagcc gtcaaacgcg ccaaagaagt cggtgtgcgt aaagtattag
     7681 gcgctaatcg cttccaattg gtcgctcaat tttacggtga aacgattttg atcacgctgt
     7741 ttgccgtcat ccttactttc gggcttaccg aatttagttt ccctaaagtt atggaatggt
     7801 tggatctgac tattccttcc gtcttgatct ggaacgccga cagtcttttg tttattgccg
     7861 cactctcggt gatggttatc tttttagccg gcctttaccc ggcgctcgtc ttatccggat
     7921 tcggtcctgc acttgcgctc aaaagtaaga tcacttcgta tcattccgga ggcctgtatc
     7981 tgcgcaaagg tctcgtcgtt ttgcagtttt cgatttcaca actgctgatc atcggcacga
     8041 tcattgcgac gacgcagttg gattttttca acaaacaaga ccttggtttc aataaagaag
     8101 ctgtggtcat cgtaccgata cacgagcgga caaaaacatt acttgataat ttagagcatc
     8161 aatggaaaag ccatcccggc gtggaatcgg tgagttttgc ttacgccagt gcggcctcag
     8221 gcaatcgctg gacttccaat gtcactttct taaaaaagga tgaaccgatt gaattagacg
     8281 ttgacctcaa aatggctgat gatcactacg cggacatttt tggattacaa tttttagccg
     8341 ggcgaaacta tgtagccgga gatacattgc gcgaagttgt gatcaatgag acgatggttc
     8401 gcaaactcgg attttctaat ccacaggatg ctatcggcat tgaactcaag atcggagcac
     8461 gtcgccgcgt cagcgttgtt ggcgccgtgc gtgacttcaa tgcctcctca ctccatgatc
     8521 cgatcgctcc ttgcataatt gctacgaaca gccggtttta tcaggaggcg tccataaaga
     8581 tcaacatgca acaggcaacg gatgtcttga gacacatcga aacagtctgg aataccgcgt
     8641 ttcctcaata tgtattcgac cgacgtttcc aagatgaaac gattgccgag ttttacgaac
     8701 aagagcaaat ctataccaac atgcttcgta ttttttcggg catcgcgatt ttcatcggtt
     8761 gcctcggtct gttcggtctt gtctcattta tggccgcgca aaaaaccaaa gaaatcggcg
     8821 tacgtaaagt tcttggcgcc tcggtcacgg atattttagg catgtttgca aaagagttta
     8881 tcgcgttgat cattttggct ttcatcatcg ccgctccgct ggcttactac gcgatgcaac
     8941 aatggttgca agatttcgag taccgcatca cccttggccc ggctgttttt gccgccgcgg
     9001 taatggtttc attgatcatt gccggagcca ccgtcggtta tcaggtattt cgtgccgcac
     9061 gtgccaatcc ggccgacgct ttgaaatacg aataaatttt aatgctgtaa ttttcaggag
     9121 aaaactgatg atacgtaatt atttcatcat cgctttcaga aatctgtaca gaaaaaaaat
     9181 gtacacattt atcaatttat tcggcctgag cgtttctatc gcggtgtgca tcgtcggtta
     9241 ccttatatgg gattacagtt accactttga tcagtttcat gaaaacgccg aacgcattta
     9301 tcatatcgga accacacggg tgatcaatga acaggatcaa atttttggag tttctcctct
     9361 gccattggcc ccggcgctgg cacgggattt ttcgggcgta aaacgcgccg tacgatttgc
     9421 caatcaggta gccatcgtcc gagtcggtga taaagtattt aatgaatcca tacacttcgc
     9481 ggacgaacaa tttttcgaga tgtttcgttt tgaattggaa cacggccgct acgatgcttt
     9541 aaaaagcaaa accgatctta tcatcaccca tgacatggcg atgaaatatt tcggtgaagc
     9601 gaacgtggtc ggtcaatcca tgacgataag ttacgccaat gaagttaaac aaacttatac
     9661 catcggcggt gttctaaaaa ctcttccgcg taactcgagt ttgcgattcg gttttttgct
     9721 ttcttctgaa attttatcgg atctgggtgt tgacgaacgc aacaactgga gccacgtaac
     9781 gggcgctacc tttatcgaag tccatcaggt cgaaacaatc caacagatca aaggcagcga
     9841 gtccaaatat atcgaattgg ttcgatccgc caatccacga tttcctataa gccatctcgt
     9901 catcacaccg ctcaaggacc tggctttaga gggagaaagt ttccgaagcc acgatttgca
     9961 ggacgcaccg ccgccttctg caagaatttc tcttttcgtc acttctatcc tccttcttgt
    10021 catgtcgtgc tttaactaca tgaacacctc gctcgccatg gctacggcaa gacttaaaga
    10081 aatcggtatt cggaaagtaa tgggtagccc gcgtacacag ctgatttttc aatttttgac
    10141 ggagaatgtg ctgctttgtg ttcttgctat ctttgcagct ttcggattgg ctgagttttt
    10201 ggttccagga tggaatgcgc ttttttatga tattcaactt tccatggacg aatcgaaatc
    10261 tcccggcttg tggttatttc ttatcggcct cgtcggctta acagcggtcg gtggcggatt
    10321 gtatccggcc tatgtcatca gcgcgcacaa tccggctaaa attctgcaag ggcatcaatc
    10381 taaaactacg gcgagctgga tcatgaaagt gatgctgaca ttacagtttt cggtctctat
    10441 catagccgtc gtcggcagcg tcgtgttttc acaaaatgca gagtatcaaa aaaatttaga
    10501 cttggggtat gaacccgaaa aactcatcgg cgtggttatt cagaagagtt ctatgtatcc
    10561 ggtactttcg aatgcgctca tgcagaattc acagatcgaa agcgtatcag ccggacgaaa
    10621 tcacatttta tattcatcct ggcgaagaag tataaaagtc gttgatagag atatcgaagc
    10681 ggaattttta tatgtacgtc ccgggtatac tgaaaccgtc ggattgaaat tagccgaagg
    10741 tcgttttttt gatgtttccc aaccgcttga tttgacggag tcgatcgtga tcaaccgcat
    10801 gatggccaaa gagcaaggtt gggaaaatcc tatcggacaa accatggtta ttgattcggt
    10861 gacgtgccgt gtcgtcggtg tcatagaaga tttttatatt cgcggcccgt tccggccgat
    10921 actaccctgc gtttttaaag cggccaacga agacagttat agatttgtaa tcgtcaaagc
    10981 cgatcataat aacatccatg cggcgatgcg ttctgtggaa gaaacatgga aaaaactctt
    11041 tcctgagatg ccctttgacg ggttttatca ggaaacacaa accgctaatg cggccgaact
    11101 tagcaagagt atcatgctcg tgtttttctg cgtagcgctt atggcgatca ttatttccgg
    11161 tatgggtctc tttgcgctgg tgtcactaaa tatcgtacgg cggaccaagg aaatcggtat
    11221 ccgtaaagta ctgggcgcat cggcactaca gatcatgcgt atgatgaatt cggattttat
    11281 tattctttta ttgatcgctt ccgtcatcgc cgatgtagcc ggctttttcc ttttcaaagc
    11341 gcttctggga agtatttaca gataccacgt ggaagtcgga atcatggctc tcatactggc
    11401 caacattgca gttctcctga tcggattggc gacgatcgga ggtcgtgttc tcaaaacggc
    11461 gcgcgccaat ccggtaaacg cattaaaata tgagtaaaaa ttaactgtat tgtatactgg
    11521 agtctcatga gtcgtaatta tttacttatc gccattcgaa accttttcaa acaaaaaggt
    11581 tttgcattga tcaatacatc agggttagcc gttggcatcg ccggttgttt actgattctt
    11641 ttatttgttc gtgatgaatt aagttatgat caataccatt ccaaagcccg tcgcattttc
    11701 cgactacaaa ccaatgtaga tgtttccggc aacttactta cactggccac tacttcctat
    11761 cctatggcgg aagccttgaa aactgatcac tcacaagtgg agtctgctat tcgtgtaatg
    11821 cggtggaatg atccgatact atctcaaggc gacatacgct tgaaagaaac gtcaatgctc
    11881 tgggccgacg ccggtgtttt cgatgttttt tcatggccca tgatatacgg cgacccgaaa
    11941 acggctcttc aagaaatcaa ctccgtcgta ctcagtgaaa gcgcctccaa aaaatatttt
    12001 ggcgatgaca atcctatcgg aaaaattctg cgctacgatg gtcgtattga tttattagtc
    12061 aaaggcgtga tcaaagatat accttataac tcgcatgttc gatttgaagt cctggccaat
    12121 atacaaactc tgctggacgt cgtaggccga gaaagcatga gccactggca tgccttttac
    12181 caactgcaga cctatctgct attgcgaaat actttcgatg cccaatcgtt gatcgaaagt
    12241 ttacccagct ttgttgacaa atacctcaag ggggagtttg cggaatctgt cggtcgaact
    12301 tatacaatgg aactaacgcc cttgaccgaa ctgcacctca gtacatcccg caaaggtgaa
    12361 ttttcaaccg tgggcagcat gaattatgtt tatgtattct ccggcattgc tttttgcttg
    12421 ttatccatag cgtgtattaa ttttattaac ctctctacgg cacacgcaac aaagcggcac
    12481 cgtgaaattg gcttgcgaaa agttcttgga gccaatcgct ctcaactgat ccgacaattt
    12541 ttaaccgaaa caacattact tgtttttatt tccataacag tagcattgtt attgatagat
    12601 attgtattgc catattttaa cacgcttgcc ggaaaacatt ttacattttc aatttttgcg
    12661 gattacacga ccttacttac gataccggga ttggtcgtga tcgtagggat attagccggc
    12721 atgtatcctg cattttattt atcgcgtttt aacccaatcg agtccgtgcg aggatcacat
    12781 tattcgggca ctaaaaaaac gcgcttcaga cacgctttgg taagtatcca atttgtcgta
    12841 tcaatagttt tattgatcat aacggccgtt gtttttaggc aactggattt tattcaaaac
    12901 aaaaatatcg gcatagacaa aaatcaaaca cttgttatac cggtcacgga ccaacaaata
    12961 cgcgcgcgtt accactcgtt acgtgagcaa ctcacaaccc agccgggcat tgaaaatgtc
    13021 gctatatcag gttttatgcc gggtattaga gtgcccggat cgccgttgca aaaaataccc
    13081 actggaccat cagacaagtg ggagttttcc actatccctg cggatgaaaa ttttgtccct
    13141 actctcggca tcaatatcat agccggtcgt aattttacta aacttaatcc cacggacagt
    13201 gctgaaagca tgctgatcaa tgaagcagcg gctcgtgact taggttggac accggaagaa
    13261 gcgatcggta aacctttggc ttggggcgga gatcccggat tcaatgttcg ggttatcgga
    13321 gttataaaag atttcaacta tacctcacta catcagccga tcaaaccact agtcattatc
    13381 ccgacggcat tatggccaaa tagttataat tttctcagca ttcgcatcaa taccggccac
    13441 ctctccgaaa ccattcatca tattaacacc gtttgggaac aacgaatgcc ggagacgcca
    13501 tttatttatt catttctcga cgatgatttt tcaaaactat atgccggtga aaacaaattg
    13561 ggtattattt ttctgatctt cgcatctctt gctatttttg tcgccagcct aggtctgctg
    13621 ggtttggcta catttgtatc cgaatctaga agaaaagaaa tcagcatacg taaagtagta
    13681 ggcgctgacc tgagaacttt gatcatatta ttgctcggtg attttctgat gccgattacg
    13741 atttccactc tcataagctg gccgatagcc tactatatcg catcggcttg gctccaaaat
    13801 ttcgcatatc gtaccgaacc gacttggtat gtttttgtcg cggcgacttt gttttcaata
    13861 ggtatagcag ctctgatgct catacaatat gtaatcaaga cagcccgggt caatccggcg
    13921 gtaacgctca agtcggaata gacgttttct atttatcaca acctctcaag aatcgtgagg
    13981 ttttatgttt aaaaattatt tccaaacgcc aatccggtaa acgcattaaa atatgagtga
    14041 tcaaggataa tattatggta aagaattttt tgatcgtggc catgcgcacc atgcgcaaac
    14101 acaaatcgta caccatcatg aatgtgaccg gcctcgcaac cgggttggcg tgcttcatgg
    14161 tcatagccat gtatgttcgc cgcgaaacgg cgtttgatac ctttcataaa aaccccgacc
    14221 gcctttttcg tgtagcgctg tatcgggaaa acaccggctc tccacaacct atcgcaaccg
    14281 tatctcacag tgtcgccgac atccttcgcc gcgaatcccc gggtattgaa tccatggcac
    14341 gtttatttcg atttgatcaa gacgccgtca ttctggcggg cgaaaaaaaa ttcaccgagc
    14401 cggatttgat gttcgccgaa ccggatgttt ttgacctttt gagtatcgaa ctgctgaatg
    14461 gcagccctgc taatgcttta aaaaatccta cggatgccgt tttatcggaa gccagtgcac
    14521 gcgcctattt cggacatacc gacgtgatcg gacagagtat ccgcgttcgc atcaatacac
    14581 gctacgccga tttcaagatt acgggcgtat tcaaaaacta tccttccaat tcgcattttt
    14641 ataccaacgt attattggga tttgacggag taaaaaatta cgtcggtgat gtggattttt
    14701 ttcaaagctg gtttaattat cccatgtgga catacctgcg cattaaggat atccaggacg
    14761 aagtatcggt taatgccgca ctccatcaga tcgttgaacg ccactttcct caaacgcgca
    14821 agccggcgca catgcagctt cagcgcatca cggatatcca ccttcattca caaatgagcg
    14881 gagaaattag accgaatagt aacatttttt acatctacac gtttacggtt atcgggctac
    14941 ttcttttgat cgtagcttgt gtgaactaca tcaacctttc gaccgctcaa tcggaacaac
    15001 gtacgcgcga agtcggcgtg cgcaaagtga tgggcgcccg ccgcagggaa ttaatatttc
    15061 agtttttgat ggaatccgtt ctgcaaagta tgcttgcatt gggtcttgct ttgggattgg
    15121 ccgaagtgct ggcatccgtt ctcaaccgat tcacggatct ccaactcacg ttcgattatt
    15181 cggatccgtt tttatttata ttttcggcgt ttctgatcgt ggtcgtcggc gtcggtgcag
    15241 gtttttatcc ggctgtcgtt ttgtccggtt atgcgccaat ccgaaattta agaatgaaag
    15301 cttttcggcg ccggtttcct cttcgaaaaa tattagtcat tgttcagctt tcggtgaccg
    15361 ctgcactcat cagcgcggtt tggatcattc agcttcagat caactacgtg cgctccaaag
    15421 aacttggatt taataaagat cacaccgttt tgatacgtgc gatcggaagt acggtagctt
    15481 ctaatctgaa ttacgatcgt ttcaaatacc aatgtgaaca attacccggt gtgatttcag
    15541 taacacgtca ttcatgggtg gcaggcgaag ggtttcgaat tcggtccgtc ggttttgatt
    15601 ctttttcgga agagcagcgc caaccgatac aatttatctt tgtaggcgag gattatctca
    15661 aaacgtacga tctgaaactt attgaaggcc gtgatttttc cgcacagtac ccggccgata
    15721 cagccggcgc atatatcgta aacgaagccg ccgtcaaagc atacggactg accaacccta
    15781 tcggacgcat agccgtcagc ggtgatcgcg gcccggtgcg cggacccatc gtaggagttg
    15841 tcaaagattt tcattttgcg agtttgcacg aaaccatcga accgatggtc atcggattat
    15901 tccgattccc gatgccttac atctccgtac gatttgacgg ccaagcaacg ccggaaacca
    15961 tcggcgccat cgaacgttta tggaataaca ttgatacgga acgcccgatg gactatagct
    16021 ttttagatac acgtatcgag cgcgtctatc gttttgaaaa ccaactttcc gccataacca
    16081 aattattcac gattttatcc atgatcatcg gctgcatggg tttattcggc cttatcgcag
    16141 ccatggctga aaaacgcacc aaagaaatcg gcattcgcaa agtattgggc gcttcgattt
    16201 ggggcataat cggaatgttt gcccgcgaat tttcggccat ggtcattgtc gccacgcttt
    16261 tagctgttcc gactgtgtat tccggtctat cccaatggct cgaaggattt gcatatcgtg
    16321 tggagctgac ggctgaaccc tttatcgtat cctttatcgc gatcttgttt ttaacggtta
    16381 ttacggtcgc tcatcatgca ttgaaggccg cgaaaactaa tccggcggta tcgctcaagt
    16441 cagagtagac gatttctatt tatctcaacc tctcaaggat cgtgagattt tatgtttaaa
    16501 aaaatcataa caacgtaaaa atcgcatagg atatctatgt tcaaagttta cctgaaaatt
    16561 attttacgaa actttctgaa agaacgttcc ttcgtctgga tcaacctgat cggattatcg
    16621 gtcgggctgc tctgttgttt tacgatctta ctctacgcgc gtaaggaatt ggcctacgaa
    16681 aattttcacg ttaacaagga tcacatttac cgtgtattgc cgcgaatcca aggcggtgcc
    16741 aacgattatg aacaaatttg gacgccggca tggcttgccc cgtccgctaa aaataaaatt
    16801 cccgagattc gcctcgcatc gcgatacatc catttgggat cggaacaact gatcacacat
    16861 gaaaaccaac gtatgcttac ggacggatta gctttggccg atccggattt tttacacatg
    16921 ttctctttta cggaaccgtt cacgtccgat atgaatccgc ttagcgatcc ttcggcactg
    16981 atcgtctccc gttccgctgc gtatcgtata tttggagatc aatctccgat cgggaaaacc
    17041 ataacgctca atggttcaca cgtttttacg atcaaagccg tttttacgga tgtaccggtc
    17101 gaatcccact tgcaatttga aatgttgggt aattttcagc gcattcgcga aatctgggga
    17161 atgaacacga caccggaaga aaataccagt tggaattttg caacctacct gatgacaacg
    17221 cctcaagccg acatcagcgc ggtacagtct aaggtattga atattttcga agaacgtgca
    17281 ccgcgaaaaa atcagcgcac ggtccgacta cagtctctca acgatattca tttcacgcaa
    17341 ggtatccgcc gtgataacgc c
//
LOCUS       DAOQGF010000070        17217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1530_length_17217_cov_6.865225, whole genome shotgun sequence.
ACCESSION   DAOQGF010000070 DAOQGF010000000
VERSION     DAOQGF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_17217_cov_6.865225"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK937_10285"
     CDS             <1..258
                     /locus_tag="PK937_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33002.1"
                     /translation="NYPNPFNPSTHIRFAVPQASNVRLTVFNLLGQKVRTLIEDYRSS
                     GSYGVVWDGRNDAGLGVASGIYVYRLEAAGRIVSRKMMLMK"
     gene            279..878
                     /locus_tag="PK937_10290"
     CDS             279..878
                     /locus_tag="PK937_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33003.1"
                     /translation="MKKYILCFLTLWVLSCGGDSDSSVSYSETVERGATALNEGSYAE
                     AIKHFSDALAMNADRPEAFAGLGWAYLFSDSVEASEAAFSDGSMLANPGADLQAGYAF
                     LLGVKKNHASSNARIVSVLAAEPQWSLSYGLNIDYKDLYLLKAENHFLLGEYASSLTA
                     VKQIQSAFNVNTNTAEGIAQLASKIESLKLLTKTLARQM"
     gene            1292..2515
                     /locus_tag="PK937_10295"
     CDS             1292..2515
                     /locus_tag="PK937_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33004.1"
                     /translation="MKRILFTFAALIVLGGSAFAGIHSVPASAEDIQATYPNLVMPED
                     MISGEVNCMLVAGDVLYVGGRFEKMDRRVVNNVVKWDGTKWSGLNKGVDGVVYSMAMI
                     GTDLYVAGDFSYVDVNIEAGKIARWDGTKWNALSPQTVDRQIYALAVLGNDLIIGGNF
                     QKVGGTIETKGVAKWNGKAWSDVGMAKFDRTVLTLTVMNNEIYAGGFFTLVGDEPATS
                     VAKWDGKAWTEVGNKGINGTVTFMTNDGKNVYAGGKFTNAGDGTVLNRVAMFDGQKWQ
                     PLGEGVDGDVKSIHISGTDVYVSGTYREAKGGTAAAPAKEETAKGKDKKKGSKEDKGK
                     AKAKGPVQTQGIAKWDGNGWTGYPVIGKYAVGTFASAMFKGTLYVGGNFMANEGAVST
                     GTAKLENGAWGPVSK"
     gene            2604..3362
                     /locus_tag="PK937_10300"
     CDS             2604..3362
                     /locus_tag="PK937_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33005.1"
                     /translation="MKRIVFFLISTIAIMMGCDPKQNSDLTIPDVTNVLIMSPVMDFT
                     DVANTFPTYTKGIQYTTWNCNDSVPSPAYLRTFDAVLLFSNNGSSRADAVGDSLYAYV
                     MDGGALILGVFYNYHRSDTTITQYWGDLETIDPFIGRGNYYGNDTLVLDGNHDLLNGL
                     DTLATYYHANNLRIRSGAKVAAFYRSGNPAIAYNKPNGRIVAVNAFPAEFKYNAVTRV
                     EFYRLWENALLYAAWGTTDDRPNDHMPNEASIKE"
     gene            complement(3486..5096)
                     /gene="nadB"
                     /locus_tag="PK937_10305"
     CDS             complement(3486..5096)
                     /gene="nadB"
                     /locus_tag="PK937_10305"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNH33006.1"
                     /translation="MPSSYTSDFLVIGTGIAGYSCALKLAEMGTVAVITKKNHRESNT
                     NYAQGGIASVIDPDDSFDAHVRDTLNAGAGLCHRDAVELIVRQGPSAIDELIRIGVEF
                     TRHDDRLDLGKEGGHSTHRIVHAKDLTGREIERALIEAVKRHPNIQVFEDHIAVEVIT
                     EHHLLQKLTAYANIHCYGAYALDIPKNIVKKFIAKVTILASGGCGQVYKHTTNPKIAT
                     GDGIAMAYRAGARVGNLEFMQFHPTSLYHPDGESFLISEAVRGFGAYLINHAGERFME
                     RYSPMKELAPRDIVARAIDAEMKKRGEPCVYLDLRHIEAHKTLEHFPNISRRCAELNI
                     DMTREPIPVVPAAHYMCGGVVTDLYGRTNIHHLYACGEVAMTGVHGANRLASNSLLEA
                     VVFAHRVADHIRTASDDIEHKIPEFPDWDDSGTFDTEEWVLIAHNRAEIQEIMWNYVG
                     IVRSNLRLERAERRIGMIVEETENFYKKTKVTEELLELRNLAKLARLIIRCAKSRHES
                     RGLHYTTDYPEKDDHRWLKDTITSTFDA"
     gene            complement(5106..6743)
                     /locus_tag="PK937_10310"
     CDS             complement(5106..6743)
                     /locus_tag="PK937_10310"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNH33007.1"
                     /translation="MARKKAHPKFIFVTGGVISSLGKGIAASSLGMLLKEHGYNVTIQ
                     KFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDDNMSTRNSHTTGKVYK
                     TVIDKERRGDYLGATVQVIPHITDEIRGRIRTLTNEGYDIIITEIGGTVGDIESLPFL
                     ESIRQFCLELDPQDTVKIHVTLVPFIKAADELKTKPTQHSVMKLREIGIQPDILLCRS
                     ERTLSKDMRAKIGLFCNVPSFAVIEASDVRSIYEVPLIMHNNGMDQLVLKKLDLPSKK
                     LNLAHWKNLVHKIYEPKNKVRIALCGKYTGLPDAYKSILESFIHAGVENNANVDVAFI
                     DSSKIDEKTDLRHTFAGIDGILVPGGFGERGIEGMIRAIRYAREHKLPFFGICLGMQC
                     MSIEFARHVCGFSDANSSEFSKVTKHPVIDLMESQKDIKEMGATMRLGAYPCVVKDRT
                     KARSAYGSDLISERHRHRFEFNNRFIPDFEKKGMVFSGLSPDHKLVEIIELKEHPWFL
                     GVQFHPELKSRAIKAHPLFREFIKAALGHQRTHGKKK"
     gene            complement(6819..7106)
                     /gene="gatC"
                     /locus_tag="PK937_10315"
     CDS             complement(6819..7106)
                     /gene="gatC"
                     /locus_tag="PK937_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNH33008.1"
                     /translation="MPVTRTDVEKIASLAHLEFSDADLEKFTGQLNQILTYVEQLNTV
                     DTTDVEPTYHPITYPDVFREDQVAESLPVDKVLANAPDKTWQYIAVPKVVG"
     gene            7298..8245
                     /locus_tag="PK937_10320"
     CDS             7298..8245
                     /locus_tag="PK937_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNH33009.1"
                     /translation="MARSAYFKGVFFICLSAVCWSSAGLFIKLVNLPPVHIAMYRSLI
                     AGIFLLGYILYRRHARDIDFDFSVNRHGLITAIFYFFTVSLFVTANKMTTAANAVFMQ
                     YTSPVFVILITYFFLKEKIYRAEILTVVICLGGMVLFFLEEEKSTAFVGNMIAILSGV
                     AFAFLQIYVKRSETGTAEALTADQHHLRSIFAIVIGNLVTVMMGFVSAMFLMHAVTMP
                     DALAPIFGTQFTITTSDIIGLLILGIFQLGCGYLFFAAGAKYISSVEIAIYTILEPIL
                     NPIWTFLGTGEVPGFWAIIGANLIVAAMLVNTFFNKERA"
     gene            8275..8763
                     /locus_tag="PK937_10325"
     CDS             8275..8763
                     /locus_tag="PK937_10325"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNH33010.1"
                     /translation="MGSGKTTVGAFVAQRMNFPFFDTDHIIEKKTGRTVSDLFADFGE
                     TYFRDAETVCLKETVQNYHQGIFALGGGALLRRENQVLVKNSGALIHLAVSHAEIIRR
                     IGRDGNRPLWDADRLDRLMEAREEGYRTADRSFDTDGFTAQSVADAVVRYIMNPPGIE
                     KK"
     gene            8873..9115
                     /locus_tag="PK937_10330"
     CDS             8873..9115
                     /locus_tag="PK937_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33011.1"
                     /translation="MARLTASEWVARENNCSEPEAEVMIEDDPDLEYDYYIRYCLQEL
                     NLSPDEIIRGFNSDMTEAQERFIEDFKGEDEMELEQ"
     gene            9299..10666
                     /locus_tag="PK937_10335"
     CDS             9299..10666
                     /locus_tag="PK937_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33012.1"
                     /translation="MRLRHRLFIIAVMMYAINAWAQDGATTTPQDSAVAATPQETPLD
                     STQTLTPKLTELFTKLRTDSNNAALLYDIYKEYLKIGRRNYAVEFINASLRKDPTNMD
                     VLYEKGELLLEMGKKKSAYEAFLLVMRDFKGQAYLDRIGPKFVSNFKITQITNNKFND
                     VMASYAPDGKSIIFQSDRSGNWDIFTMNPDQGESSVKKLTDDLEADENPAYSTDGKWI
                     AFASTRDDKSTKEYKSRELYFMDKTGKRVTRLTSSYGGDNWSPIFLDTVTIAFSSDRS
                     DFSSTPFAKKNTGIYTIERNGSFLFKMFYQDKMNYTDVAYKAVSNQFVFAQRADDSDF
                     DLYMTSTDTKEKPVNLTNHPKNDLQPSVSKNGNFIAFVSDRDGNYEVYRMQADGRDQT
                     RITNDDGDDVYPKFSPDGNKIIFSSNRNGKYQLYIASTEESSAVTVQSVIAILEKRIA
                     SSSDN"
     gene            10963..16215
                     /locus_tag="PK937_10340"
     CDS             10963..16215
                     /locus_tag="PK937_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH33013.1"
                     /translation="MNKAGKSHTQIVLFLGSTAQASALTALTQWEVVSITDRNALRKV
                     TQIRSSSAILTTTYRRIASALVLKKKMMHAANLRVGFALPAAQGKRLVKEGVLKSDEV
                     IPLPFTKKNVLSFLSPTSHWPKQYDNSIASDLFLRAVESTDDAVIITDKDGHILYVNS
                     GFEKNSGYGRDEVLGKNPRILKSGYHEQAFYSGMWEKILSGSVFRNIQYNRRKDGSII
                     PVEHTISPVRNEIGEITHFIGIWKDISGKIRSENETRQAQDQLKQNEIKYQIIADNTF
                     DWEFWLDTDGSYIYSSPSCERITGYPPVSFTTDRDFLQRIVYAEDRAAFLAHQQDIQQ
                     RRLPGNLDFRIVCKGGVIKWIGHVCQPISDAAGVFRGVRGSNRDITERKLAELALRQK
                     ESQAESLLRLARQLERVAGFGEAAQAALTEVQRLVGYQRLWVYVFSEDRSEAEVLAEA
                     GMPRPFAPKRLDIKNDAMLSAVARGTGAVVIPDAAEDARTNKKIVQSTGVRTIVNIPV
                     HLSEGRLASIGMGTYDDEGVRVPDESALQYLTTLASHMSATIDRLLSQMVRQSAEDSL
                     RASEKRYRTLIDQAADGIFVATRDGQIEDVNPAGCRLMGYTVNELLKQPFYGLLDAEE
                     LIRQPLKIRQLEKGQSILSHRRLVRKDGTHVEVEISAKMLDDGRIQGIVRDISERRRV
                     EKHFQALIEHAPDGVVLINAEGKFQYISPSSARMFGYEMSELSDLEPAALTHPDDVER
                     VEQLIASVIENPDIDARAKYRFKHKNGSWKWIESTFTNLLQAPEVQAIVVNFKDVTER
                     HNAEEALLHSEQKYRTLAETSQDMVLLFENNGYIIYANPAVEQNLGYVQGALSGKMIS
                     TILPGNADYDAISYLTGGASHEIGAVSREIDFLHLNGEVIPFDVRTSKAVSSGTPVIV
                     CIGRDNRSRRKYENHIREQLRYARALNRMAASIMNSDGTAETLLREMTTICGETIDIE
                     RALLFDIRITGGEAVSIAKWIRPDFTDFAPPQEVYPTSVFASSISRLHSTRTCITSTV
                     DAPHACFVEEGSAEILHRVMKVQSLLWYPFSFREDGFFLLVVNDMIHPRQWQEEELNF
                     LDSVSREIELALLKHRLSSEREQAEVKLSETLREFETLVEAIPDAVFFKDGDGRWLIA
                     NPAALKLFELATYPWYGKTDQQLAEAFPALRLAYEGCVRTDEAAWRNGQRYQTIEIIE
                     KPGGDLLHFDTTKIPLLQENGARLGLVVIGRDITFHKKAKEIIQEQAMILDNARDAIF
                     MTDPEGRIIYWNSQCETLYGWEKANVIGHQATEVLGHTRATDAAARILFSGDAPWSGE
                     IEHYARDGRNVIVECRKSPVRDADSAVKSFLYIETDVTERKRLEKQYFRAQRMESIGR
                     MASGIAHDLNNILSPILMAVQMFKSKLPDDTSQRLIVMLESNIERAADLMRKLLMFSR
                     GAEGAHRVVDAKKTIEEIERILVSTFPKNIRIVCNVAKDLYPITADSTQLHQVFLNLC
                     VNARDAMPSGGDLTIHFENVIFDEAYVYKEHQAKPGPYVLFTVTDTGLGIPADIIDHI
                     FDPFFTTKDLEHGTGLGLSTVAAIIKGHGGFVSVYSEVGKGTQFKIYIPASAGFHTES
                     VESRIPESLLGRGELILIVDDEPAIRKILGTILTHNGYQIVEAEDGGKAMTVFAAQMA
                     SIRAVITDISMPHMDGHAFIRMLRSIREDVPIIAMSGLPENEKFIQKNGFKVQAFLMK
                     PYRAEKILQTLNDVLAPLRSAEQS"
     gene            16245..>17217
                     /locus_tag="PK937_10345"
     CDS             16245..>17217
                     /locus_tag="PK937_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33014.1"
                     /translation="MTILIVDDERTSVDALSLALGQSGYSVVTALNGKAALTILAGQS
                     PDLIISDIMMPELDGFGFIEAVRRQPRLASIPFLLMSGDKDPSLTAKAIQMGAIDFLP
                     KPINLNLLFLKIQALFRFVEAQRSSGILRSGSLVLGKIEELIASAEKEGIHGILTIQS
                     DHGVGSIEFNRGVCETIVCGELKDSEALETMLQWSSGSYYLARRPTELTESENKIPVS
                     VHSTKEGNAMKSEDFSKPIAIAEDIYFVGDRKESRILQINPFLRVYNNKVNFLVDPGP
                     TIDYTTISGKVGEVIGDVSKVNLYSLNHQDPDVASNTFFLKSANPKAI"
BASE COUNT         4535 a         3729 c         4320 g         4633 t
ORIGIN      
        1 aattatccga atccatttaa cccgtctacg catatccgat ttgctgttcc gcaggcgtcg
       61 aacgtccggc ttactgtatt taatttgctg ggtcaaaaag tgagaacgct catcgaagac
      121 taccgttcat ccggttcgta cggggttgtc tgggacgggc gtaatgatgc cggattgggc
      181 gttgcctccg gaatctatgt atatcgtctg gaagcagccg gccgaatcgt ttctcgaaaa
      241 atgatgttga tgaaataacc caaattaccg gagacgttat gaaaaaatat attttatgct
      301 ttcttacgct atgggtgctg tcctgcggcg gagattccga cagttctgtt tcgtatagtg
      361 aaacggttga acgcggtgca acggccttaa acgaaggttc ttatgcggaa gcgatcaagc
      421 atttttcaga cgctttagcg atgaacgctg atcggcctga agcctttgct ggtttgggtt
      481 gggcatacct gttttcggat agcgtggagg cctccgaagc ggcattctcg gatggcagca
      541 tgcttgccaa tcccggagcg gatttgcagg ccggatatgc gtttttattg ggcgttaaaa
      601 aaaatcatgc atcttccaac gcgcgtatcg ttagcgtatt ggccgccgaa ccgcaatggt
      661 ctttgagcta cggcttgaat attgattaca aggatttgta cctgcttaaa gcggaaaatc
      721 attttcttct cggtgaatac gcgtcgagtc ttactgcggt caaacagatt caatcagcgt
      781 ttaatgttaa tacaaacacg gcagagggca tcgcacaact agcctctaaa attgaatctt
      841 tgaaactgtt aactaaaact ctggcgcgtc aaatgtaatt ttgacaaaaa aaactaaagc
      901 tttaattatt ataaaacgcc tgatctgaaa agaacaggcg ttttttgtta ctaaaacctg
      961 ccttttcgtc aacccaatcc cccttttcgt caatcaaaag acccccaatc ccgattttca
     1021 ccttatgtta atggcgtgtt tacaaactta tgggagaaca agtatgccaa acacaatatc
     1081 tgcattattg cacaaccgct tcattcggcc ggcttctgca ttatatcatc tttctttgtc
     1141 agcggataaa agttttatca cataaccgcg caagtacctc ccataagttg gccggcgaag
     1201 acgttcaccg gtcggcttgc gcggagtttt ttgcgtttat tcacgtaggt tcggttctca
     1261 ttatcatata taacaagaaa ggaaaaaaca catgaaacga attctgttca cgtttgccgc
     1321 gctgatcgtc cttggtggat cggcatttgc aggaatccat agcgttcccg catccgcaga
     1381 ggatattcaa gctacgtatc ctaatctggt catgccggag gatatgatta gcggtgaagt
     1441 caattgtatg cttgtagcag gcgatgtatt gtacgtcggc ggacgatttg aaaaaatgga
     1501 tcgccgtgta gtcaacaacg tcgtcaaatg ggatggcacc aaatggtccg gtcttaataa
     1561 aggcgtggac ggtgtagtgt attcgatggc catgatcggt accgatttgt atgtggccgg
     1621 tgattttagt tatgtcgatg tcaatatcga agccggaaaa attgcacgat gggatggtac
     1681 taaatggaat gcgctgtcac ctcagaccgt cgatcgccag atttatgcct tggcggtgtt
     1741 gggcaatgat ctcatcatcg gcggaaattt ccaaaaagtc ggcggtacga tcgaaaccaa
     1801 aggtgttgcc aaatggaacg gcaaagcttg gagcgatgtg ggtatggcca aatttgacag
     1861 aacggttttg actctgaccg tgatgaataa tgaaatctat gccggcggtt tttttacgct
     1921 tgtaggcgac gaaccggcta cttccgtggc gaaatgggat ggtaaagcat ggacggaagt
     1981 cggcaataaa ggtatcaacg gcacggtgac attcatgacc aatgacggaa aaaatgtgta
     2041 cgccggtgga aaatttacca acgccggcga cggtacggtg cttaaccgcg tagctatgtt
     2101 tgacggacaa aaatggcaac ctctcggcga aggtgtggat ggtgatgtaa aatcaattca
     2161 tatctccgga acggatgtgt atgtgtcggg tacttatcga gaagccaaag gtggtaccgc
     2221 cgctgctccg gcaaaagaag aaaccgctaa aggcaaagat aagaaaaaag gtagcaaaga
     2281 agataaaggt aaagccaaag ccaaaggccc ggtacaaact cagggcatag ctaaatggga
     2341 cggcaatggt tggaccggat atcccgtaat cggtaaatat gctgttggta catttgcttc
     2401 cgctatgttt aaaggaacat tgtatgtcgg cggaaacttt atggccaatg aaggcgccgt
     2461 cagcacgggt accgccaaac ttgaaaatgg cgcttggggg ccggtttcta aatagttgag
     2521 gttatcgtat gaataacttg aaggagctat gtttctatag ctccttcgca tttaataaaa
     2581 catcaggacg gagatataaa cacatgaaac ggattgtttt ttttctaatt tctactatag
     2641 caattatgat gggttgcgac cctaaacaaa attccgatct gacaatacca gatgtgacta
     2701 atgtattgat catgtcgcct gtaatggatt ttacagatgt agctaatacc tttccgacct
     2761 atacgaaagg tattcaatac acaacttgga attgtaatga ttccgtgccg tcaccggcct
     2821 atctgagaac atttgatgcc gtgctgttat ttagtaataa tggcagcagc cgcgccgatg
     2881 ctgtcggtga tagtctttat gcgtatgtga tggatggcgg cgcattgata ttgggtgtat
     2941 tttataatta ccatcggagc gatactacga ttacgcagta ttggggtgat ctcgaaacta
     3001 tagatccgtt tatcggcaga ggaaattatt atggaaatga tacgctggta ttggatggta
     3061 atcatgattt attaaacgga cttgatacat tggcaaccta ttatcatgcc aataacctta
     3121 ggatacgcag tggtgcgaaa gtggcagctt tctatcgctc gggcaatccg gctatagcgt
     3181 ataataagcc taatggaaga attgttgcag tgaatgcctt tccggctgaa tttaaataca
     3241 atgcggtaac gcgggtggaa ttttaccgtt tatgggaaaa tgcgttatta tatgccgcat
     3301 ggggaactac ggatgatcgc ccgaatgatc acatgccgaa tgaagcctca atcaaagaat
     3361 gaaagaatat gattaatgta aatcaaactg cagtcgttga ggctcaatag tattttatat
     3421 ctaaaacaaa aaaacgccgc aggagaatat cccgcggcgt tttttgtttt tatagttata
     3481 attttttatg cgtcaaatgt gctggtgata gtatctttca gccagcggtg atcgtctttt
     3541 tcgggatagt cggtcgtata gtgcaagccg cggctttcgt gacgggattt ggcgcagcga
     3601 atgatcaggc gcgccagttt ggctaaatta cgcagttcca gcaactcttc cgtaactttg
     3661 gtttttttgt aaaaattctc cgtttcttcg acgatcatgc cgatacggcg ctcggcccgt
     3721 tccagacgga gattggagcg cacgatgcct acatagttcc acatgatttc ctgtatttcg
     3781 gcgcgattgt gtgcgatcag tacccattct tccgtgtcaa aagtaccact gtcatcccaa
     3841 tccgggaatt caggtatctt gtgttcgata tcgtccgatg cggtgcgaat atgatcggct
     3901 acccgatgtg cgaaaacgac cgcttcgagc agggagttgc tggcaagacg attggcaccg
     3961 tgtacgccgg tcatcgccac ttcgccacaa gcatagagat gatggatatt ggtacggccg
     4021 tacaaatccg tcaccacgcc gccgcacata tagtgtgcag ccggtaccac cgggatgggt
     4081 tcgcgtgtca tatcaatatt aagttcggca cagcggcggg aaatgttggg aaaatgttcc
     4141 agcgttttat gtgcttcgat atgccgcaaa tccagatata cgcacggctc gccgcgtttt
     4201 ttcatttcgg cgtcaatcgc acgggccact atatcacgcg gcgccagttc tttcatgggt
     4261 gaataacgct ccataaaacg ttcgccggca tgattgataa gataggcgcc gaaaccgcgt
     4321 accgcttcac tgatgagaaa ggattctccg tcgggatgat acagtgaagt cggatgaaac
     4381 tgcatgaatt ccaaattacc aacgcgggcg cccgcccgat acgccatcgc aatgccgtcg
     4441 ccggtggcga ttttcggatt cgttgtgtgt ttgtacacct gaccacaacc gccggatgca
     4501 aggatggtta ccttggcgat aaattttttt acaatatttt tgggaatatc gagcgcgtat
     4561 gcgccgtagc agtgaatatt ggcatacgcc gtgagttttt gaagaaggtg atgttcggtg
     4621 atgacttcga cggcgatatg atcttcgaat acttggatat tggggtggcg tttcacggct
     4681 tcgatcagag cccgttcgat ctcgcggccg gtcagatctt tagcatgcac gatacgatgc
     4741 gtgctgtgtc cgccttcttt gccgagatca aggcggtcgt cgtggcgtgt aaattcgaca
     4801 ccgatacgta tcaattcgtc aatcgcagag gggccctggc gaacgatcag ttcgaccgca
     4861 tcccgatggc acaaaccggc gccggcattg agggtatccc ggacatgtgc gtcaaaacta
     4921 tcgtcgggat caatgacgct ggcaatgccg ccctgtgcgt aattggtatt gctttcgcgg
     4981 tgattttttt tggtgatgac ggcgaccgtt cccatttcag ccaatttcaa tgcacaagaa
     5041 tatccggcaa tgcctgtgcc gataacaagg aaatcggatg tgtatgacga cggcatcggt
     5101 gcgaattatt tctttttacc gtgtgtacgc tgatgcccca gagccgcttt gataaattcg
     5161 cggaacagcg gatgtgcttt gatcgcgcgt gatttgagtt cgggatgaaa ttgtacgccg
     5221 agaaaccaag gatgctcttt gagttcgatg atttcgacca acttgtgatc gggagaaagg
     5281 ccggaaaata ccattccttt cttttcaaaa tcagggataa atcgattgtt aaattcaaac
     5341 cggtggcggt ggcgttcact gatgagatcc gatccgtagg cggagcgcgc tttggtgcga
     5401 tctttgacga cgcacggata cgcaccaagg cgcatcgtgg cgcccatttc tttaatatct
     5461 ttttgagatt ccatcaaatc aatcaccgga tgtttggtga ctttggaaaa ctcgctgcta
     5521 ttggcatccg aaaaaccgca gacatggcgg gcaaattcga tgctcatgca ctgcatgccg
     5581 aggcatatcc cgaaaaacgg caatttatgt tcccgtgcat aacgaatggc tcggatcatg
     5641 ccttcgatcc cgcgttcacc gaaacctccc ggaacgagta taccgtctat gccggcaaac
     5701 gtatgccgta aatcggtttt ttcgtcaatc ttgctggagt ctatgaaagc gacatcgaca
     5761 ttcgcattat tttccacacc ggcatggata aaggattcta aaatactttt ataggcatcg
     5821 ggtaggccgg tatatttgcc gcacaacgcg atacgcactt tatttttcgg ttcataaatt
     5881 ttgtgaacca gatttttcca atgtgccaga ttgagttttt tgctcggcag atcaagtttt
     5941 tttaacacca gctgatccat gccattgttg tgcatgatga gcggcacttc gtatatggag
     6001 cgcacatcgg acgcttcgat cacggcaaat gaaggtacat tgcaaaataa accgatcttg
     6061 gcgcgcatgt cttttgacag cgtgcgttcc gagcggcata gcaaaatatc cggttgaata
     6121 ccgatttcac gtagcttcat cacgctgtgt tgagtcggct tggttttcag ttcgtccgcc
     6181 gctttgataa agggcacgag cgtcacatgg attttgacgg tatcctgcgg gtccaactcc
     6241 aaacagaact ggcggataga ttccaaaaat ggcagacttt cgatatcgcc gaccgtgccg
     6301 ccgatttccg taatgatgat atcgtaacct tcattggtca gtgtgcgaat acggccgcga
     6361 atttcgtccg tgatatgggg aataacctgt acggtggcac cgagatagtc accgcgccgt
     6421 tctttgtcaa tcacggtttt ataaacctta cccgtggtgt gcgaattgcg ggtgctcatg
     6481 ttatcgtcaa taaagcgctc gtaatgcccg agatcaagat cggtttccgc gccgtcatcc
     6541 gtgacgaaga cctcgccatg ctgatatggg ctcatcgtgc cgggatccac attgatgtaa
     6601 ggatcaaatt tttgaatggt gacattgtaa ccatgctctt tgaggagcat gccgaggctg
     6661 gatgccgcga tgcctttgcc gagggacgaa atcacgccgc cggttacaaa aataaatttc
     6721 ggatgtgctt ttttacgcgc catagttttt taaagggaaa cttaatgatg aaaggtaatt
     6781 ataaaaaaag agacacgctt ccgtgtctct tttggttttt agccgacaac tttcggcacc
     6841 gcgatatact gccaggtttt atccggtgcg ttagcgagaa ccttatccac cgggaggctc
     6901 tcggctacct gatcttcacg aaatacatcc gggtaggtga tcggatggta ggtgggctcc
     6961 acatccgtcg tatctacggt attgagctgc tccacatacg tcagtatctg attgagctgg
     7021 ccggtgaatt tttccaagtc cgcatcggaa aattccaaat gcgcaaggga agctattttt
     7081 tcgacatcgg tacgtgtaac cggcataggt tgcgctcttt tttttaacgc gggcaatata
     7141 tgtgaaagcc ccgtttttgt caaatttatt ttccttacgt caaaggacta aaaacagatt
     7201 accacaaaga tatttagcga aaaaccggta gcgcaattta ccgttgtgaa tcacaaggaa
     7261 tgctttatat tccccaaaat caaaatcgga attatctatg gcgcgttctg cgtatttcaa
     7321 aggcgttttt tttatttgtt tgtccgccgt gtgttggagc agcgcgggtt tatttatcaa
     7381 attagtcaat ctgccgccgg tccatatcgc gatgtatcgt tcgctgattg cgggtatttt
     7441 tttgttaggt tacatcttgt atcgccgtca tgcccgggat attgattttg atttttcggt
     7501 caaccggcac ggattgatca cggccatttt ttattttttt actgtttcac tgtttgtcac
     7561 ggctaataaa atgaccacgg cggccaatgc cgtgttcatg caatatacct cgccggtttt
     7621 tgtcatactg atcacatact tttttttaaa agaaaaaata taccgggcgg aaattcttac
     7681 ggtggttatt tgcctcggcg ggatggtgct tttctttttg gaggaggaaa aatcaaccgc
     7741 ttttgtcggc aatatgatag ctattctttc gggtgttgcg tttgcgtttt tgcagattta
     7801 cgtcaaacgt tccgaaaccg ggacggccga agccctcaca gccgatcaac atcatttacg
     7861 ttcgatattt gcaattgtca tcggtaatct agtgaccgtc atgatgggtt ttgtatcggc
     7921 catgtttttg atgcatgccg ttacgatgcc ggatgcgttg gcaccgattt tcggtacgca
     7981 atttaccatt acgacatccg atatcatagg ccttttgatc ttgggcattt ttcagctggg
     8041 gtgcggttat ctgttttttg ctgccggcgc caaatatata tccagtgtcg aaatcgccat
     8101 atacacgata ttggaaccca ttctcaatcc gatctggact tttttgggaa ccggtgaggt
     8161 gcccggcttt tgggccatca tcggcgccaa tctgatcgtt gcggctatgc tggttaatac
     8221 tttttttaac aaagaacgcg catgaatacc cgtcggatat ttatatgcgg atttatgggt
     8281 tccggcaaaa cgacggtcgg cgcattcgta gcgcagcgga tgaattttcc gttttttgat
     8341 acagatcata tcatcgaaaa aaaaacgggc cgcaccgtct ccgatttgtt tgccgatttc
     8401 ggcgaaacgt actttcgtga cgctgaaacc gtctgcctca aggaaacggt gcaaaattac
     8461 catcagggta tttttgcgtt ggggggtgga gcgttactac gccgagaaaa tcaagttttg
     8521 gtcaagaact ccggtgcgtt gattcattta gcggtttcgc atgcggaaat catccgtcgt
     8581 atcggacgcg atggtaaccg tcctttatgg gacgcggatc gcttggatag gcttatggag
     8641 gcacgggagg aaggataccg gacagccgat cggagctttg atacggacgg gttcactgcc
     8701 caatccgtag ccgatgcggt ggtgcgatat atcatgaatc cgccggggat tgaaaaaaaa
     8761 tgagtttgac cttgacaaac catgcatgtg ttattataat tcgccgaatt tgaaaaccgt
     8821 gtaaataatt tattttatca tattcactta acacgtacgc aaaggagctg ttatggcgag
     8881 attaaccgcg tcggagtggg tcgcacgcga aaataattgt tcggaaccgg aagccgaagt
     8941 catgatcgaa gacgatccgg atctggaata cgattattat atccgctact gcctgcaaga
     9001 actcaatctt tcgcccgatg aaatcattcg cgggttcaat tcggatatga ccgaggcgca
     9061 ggaacgcttc atcgaagact tcaaaggcga agatgaaatg gaacttgagc aataataatc
     9121 tcgggtatac aatctaagtt tgaaaaggag ccgcgcacaa cgttcggctc cttttatttt
     9181 taagaggtta tcaggggaaa aaccgcttgc atttataggg gcttttggct aaaatgcggg
     9241 caattataat acagattatc cgtttaccaa ctcccgaatt tccaggagga tttttttcat
     9301 gcggttacgc cataggttat tcattatcgc cgttatgatg tatgcgatca acgcgtgggc
     9361 acaagatggc gctacaacaa cgcctcagga ttccgctgta gctgcgacac cgcaggaaac
     9421 gccgctggat tcgacgcaaa cactgacgcc caaattgacg gagcttttta ccaagctgcg
     9481 caccgacagc aataatgccg ctttactgta tgatatttat aaagagtatc tgaaaatagg
     9541 tcgccgcaat tatgccgtcg aattcatcaa tgcgtcgctt cgcaaagatc cgaccaacat
     9601 ggatgtcttg tacgaaaagg gcgaattgct tttggaaatg ggaaagaaaa aatcggcgta
     9661 tgaagcattt ctgctggtga tgcgtgattt caaagggcag gcgtatttgg atcgtatcgg
     9721 ccctaaattt gtcagtaatt tcaaaattac acagataacg aataacaaat ttaatgacgt
     9781 catggcgtcc tacgcgccgg acggaaaatc tattattttt caatccgatc gcagcggtaa
     9841 ttgggatatc ttcacgatga atccggatca gggagagtcc tctgtcaaaa aactgacaga
     9901 tgacctggaa gccgatgaaa atccggctta ttctaccgat ggtaaatgga tcgcgtttgc
     9961 ctccacgcgg gatgataaaa gtaccaaaga atataagtcg cgcgaactgt acttcatgga
    10021 taaaaccgga aaacgtgtta cccgactgac ctcgagttat ggtggtgata actggagtcc
    10081 gattttctta gataccgtga cgattgcttt ttccagtgat cgttcggatt tctcgtctac
    10141 accgtttgca aagaagaata cgggcattta cacgattgaa agaaacggca gcttcctctt
    10201 caaaatgttt tatcaggaca agatgaatta tacggatgtt gcgtacaagg cagtatcgaa
    10261 ccaattcgta tttgcacagc gtgccgacga ttctgatttt gatctttaca tgaccagcac
    10321 ggatactaaa gaaaagccgg tcaatcttac aaatcatccg aagaacgatc tgcagccgtc
    10381 cgtttcaaag aacggaaact ttatagcttt tgtgtcggat cgtgatggta attacgaagt
    10441 gtaccgtatg caagccgacg ggcgcgatca aacccgtatt accaatgacg acggagatga
    10501 cgtgtatcct aaattttcac ccgacggcaa caaaattatt ttttcctcga accgtaatgg
    10561 taagtaccag ctttatatcg cttcgacgga agagtcttct gccgttacgg tgcagtcggt
    10621 gatcgctata cttgaaaaaa gaattgcatc gtcatcggac aattgatgct taggtgatcg
    10681 gcgacaaacc atcggcaata gatgattttg atataaagtt cgttctaaag ctatctgtta
    10741 ttagagatag tgaaatgggt tttgtaacac ggcgggattt ttaagtattt aaagtaacgc
    10801 cgtgttattc tctcataact cccatccttt ttttaaattt gtttacaatt attttttaaa
    10861 atcatgatga aaatcatgag aattattatc gtattgtatt attctaacta aattttagta
    10921 gggttctcgt ggtaatttaa aaaataatga gagtatggcg ttgtgaataa agcgggtaaa
    10981 tcacatacgc agatagtgtt atttttaggt agcacggcgc aggcgtccgc tttaacggct
    11041 ctcacgcaat gggaggttgt ttctataacc gatcggaatg cactgcgtaa agtcactcaa
    11101 atccgatctt catcggccat cctaactaca acctaccgta gaatagcttc agcgcttgtc
    11161 ttaaaaaaga aaatgatgca cgcggcgaat ttgcgtgttg ggtttgcttt gcctgcggcc
    11221 caaggaaaac ggctcgtgaa agagggtgtg ctgaagtcgg atgaagttat ccctcttcct
    11281 tttactaaaa aaaatgttct atcgtttcta tcgccgacat cacactggcc gaagcaatat
    11341 gataattcca ttgcatcgga tttattctta agagcggtgg agtcaacgga tgacgctgtg
    11401 atcataaccg ataaagatgg ccatattctc tatgtgaaca gcgggtttga aaaaaattcc
    11461 ggatacgggc gtgacgaagt gcttggaaag aatccgcgga tattaaaatc aggctaccat
    11521 gaacaagctt tttatagcgg gatgtgggaa aaaatattat cgggtagcgt attccgaaat
    11581 atacaatata atcgccgaaa agacggcagc atcatacccg ttgagcacac gatctctccg
    11641 gttcgtaacg aaatcggaga gataacgcat tttatcggca tttggaaaga tatcagcgga
    11701 aaaattcgtt ctgaaaacga aacgcggcaa gcgcaagatc aattaaagca aaacgaaatc
    11761 aaatatcaga tcatagcgga taatacattt gattgggagt tttggctgga tacggacggt
    11821 tcctacattt acagttcgcc ttcgtgtgag cgtatcacgg gatatccgcc ggtttctttt
    11881 acgacggatc gtgatttttt gcaacgcata gtatatgccg aagaccgggc ggctttttta
    11941 gctcaccagc aagatattca gcagcggcgt ttaccgggta atctggattt tcgtattgtc
    12001 tgtaaaggtg gcgtcatcaa atggattggc catgtctgtc agcctatttc cgacgctgcc
    12061 ggtgtttttc gaggtgttcg gggaagcaat cgcgatatta ccgaacgtaa actggccgag
    12121 ctggcgctca ggcaaaaaga atcacaagcc gaatcacttt tgcgtttagc gcgtcagctg
    12181 gagcgagtcg cgggtttcgg tgaagcggcc caggcggcat taaccgaagt gcaacgtctg
    12241 gtgggatatc agagattatg ggtttacgtt ttttcggagg atcgctccga agcagaagta
    12301 ttggccgagg ccggcatgcc aagaccgttt gcacctaaac gccttgatat aaaaaatgat
    12361 gcgatgctga gtgcagtggc tcggggaacg ggtgcagtgg ttattcccga cgcggcggag
    12421 gatgcacgaa ccaataaaaa aatcgtacag tctaccggag tgcgtacgat cgtaaatata
    12481 ccggttcatc tttccgaagg gcgcttagct tccatcggta tgggaaccta tgatgatgaa
    12541 ggggtgcgtg tgccggatga atccgccctt caatatctca cgactttagc gagccatatg
    12601 tcggcgacga tagatcgttt gttatcgcag atggtgcgtc aatcggcgga agattcgctg
    12661 cgtgccagtg aaaagcggta tcgtacgttg attgatcagg ccgcggacgg tatttttgtg
    12721 gctacccgtg acggacaaat cgaagatgtc aatccggccg gttgccgctt gatggggtat
    12781 accgtaaacg aattgttgaa gcaacctttt tacggtttgt tggatgcgga agaattaatc
    12841 cgccaaccgc tcaaaatcag gcaattagaa aagggccaat cgattttatc gcatcggcgt
    12901 ttagtccgta aagacgggac acatgtcgaa gttgaaatta gcgccaaaat gcttgatgac
    12961 ggacgcattc agggtattgt aagggatatt tcggagcgtc gtcgcgtcga gaaacatttt
    13021 caagcgctta tcgaacatgc accggacggc gtggtgctga tcaatgccga aggaaaattt
    13081 caatacatca gtccttcttc ggcgcgcatg ttcggatacg aaatgtcgga gttgtccgat
    13141 ctggagcccg ccgcattgac ccatccggat gatgtcgagc gtgtcgaaca gcttatcgca
    13201 tccgtgatcg aaaatccgga tattgacgcg agggcgaagt atcgcttcaa gcataaaaac
    13261 gggtcgtgga aatggatcga aagtacgttt acgaatttac ttcaagcgcc cgaagtacag
    13321 gcgatcgtag ttaattttaa agatgtcacc gaacggcata acgccgagga agcgttactc
    13381 cacagcgagc aaaaataccg cacgcttgcc gagacatccc aggacatggt tttgttattt
    13441 gaaaacaacg gctatatcat ctatgcgaat ccggctgtag aacaaaatct cggttatgtg
    13501 caaggcgcct tgtccggaaa aatgatcagc acgattttgc ccggtaatgc ggactatgac
    13561 gcgatatcgt acctcaccgg cggcgccagt catgaaatcg gcgccgtgtc gcgcgaaatt
    13621 gattttcttc acctaaacgg tgaagtaatt ccctttgatg tgcgtacaag taaagccgtg
    13681 tcgtcgggaa cgccggtcat cgtatgtatc ggccgtgata accgaagtcg ccgtaaatat
    13741 gaaaatcata tccgcgagca actgcgctac gcccgtgcgc tcaatcgtat ggccgcttct
    13801 attatgaatt cggacggtac agccgaaacg ttgttgcggg aaatgacgac catttgcggt
    13861 gaaaccattg atatagagcg cgcactttta tttgatattc gtattacggg cggcgaagcc
    13921 gtttctattg ccaaatggat tcggccggat tttactgatt ttgccccacc tcaggaggtg
    13981 tatcccacct ccgtattcgc atccagtata agccgactac acagtacacg aacctgcatc
    14041 acgagtaccg ttgatgcgcc acacgcttgt tttgtagaag aagggtctgc cgaaatattg
    14101 catcgggtta tgaaagtaca gagtttgctt tggtatccgt tctcatttcg cgaggatgga
    14161 ttttttcttt tggttgtcaa tgacatgatc cacccccgcc aatggcaaga agaagaactg
    14221 aatttcctcg acagcgtgtc gcgggaaatc gaattggcgc tgctcaagca tcgtctatca
    14281 tcggaacgcg aacaggccga agtcaaatta agcgaaacgt tgcgggaatt tgaaacatta
    14341 gttgaggcga taccggacgc tgtctttttt aaagacgggg acgggcgatg gttgatcgcc
    14401 aatcctgccg cgctcaaact ttttgaattg gcgacgtatc cgtggtacgg taaaacggat
    14461 caacagttgg ctgaagcttt tcccgctttg cgtttggcgt acgaaggatg tgtgcgaacg
    14521 gatgaggccg cgtggcgtaa tggtcagcga taccaaacca tagaaattat cgaaaaaccc
    14581 ggcggtgatt tattgcattt tgatacgacg aagatccctt tgttacaaga aaacggagca
    14641 aggttgggat tggtggtaat aggccgggat atcacatttc ataaaaaagc caaagagatt
    14701 atccaagagc aagcaatgat ccttgataat gcacgggatg cgatttttat gaccgatccg
    14761 gaagggcgga taatatattg gaacagccaa tgcgaaacgt tgtatggctg ggaaaaagcc
    14821 aatgtcatcg gccatcaagc gacggaagtt ctcgggcata cccgcgcgac ggatgctgcg
    14881 gcgcgcatat tattcagcgg tgatgcgccg tggagcggtg agatcgagca ttacgcgcgg
    14941 gacggaagga atgtcatcgt cgaatgccgc aagagccctg tgcgggatgc ggatagcgcc
    15001 gtcaaatcat ttttgtatat cgagactgac gttactgaac gtaaacggct tgaaaagcag
    15061 tactttagag cgcagcgtat ggaaagcatc ggtcgtatgg ccagcggtat tgcgcatgat
    15121 ctcaataata ttttatcgcc gatcttgatg gctgttcaaa tgttcaaatc caaattacca
    15181 gacgatacaa gtcagcgttt gatcgtcatg ctggagtcta atatcgaacg ggctgctgat
    15241 ctgatgcgta agttacttat gttttcccgc ggcgcagaag gcgcacatcg tgtagtggac
    15301 gccaaaaaaa cgatcgaaga aatcgagcgt attcttgttt cgacgtttcc caagaacatt
    15361 agaattgttt gcaacgtagc caaagatctg tatccgatca cggccgatag cacgcagctt
    15421 catcaagtat ttctcaacct gtgcgtcaat gcacgagatg ccatgccatc gggaggtgat
    15481 ctgacgatcc atttcgaaaa tgtaattttt gatgaggcgt atgtctataa ggaacaccag
    15541 gccaaacccg gcccgtacgt tctgtttaca gttacggata caggactggg gattcctgcg
    15601 gatatcatag atcatatctt tgatccgttt tttacaacca aggatctcga acacggtaca
    15661 ggtttggggc tatccacggt tgcggcgatt atcaaaggac atggcggctt tgtgagcgtg
    15721 tatagtgagg tcggtaaagg cacacagttt aaaatttaca tccccgccag cgccggtttc
    15781 cacacggaat cggttgagtc tcgtattccg gaatcgttat tgggccgtgg tgaattgatc
    15841 ttgatcgtag atgatgaacc ggccatacgc aaaatattgg gaacgatcct gacgcataat
    15901 ggataccaaa tagtcgaagc ggaggacggc ggaaaagcga tgacggtatt cgccgcccaa
    15961 atggcttcca ttcgcgccgt gataaccgat ataagcatgc cacacatgga cggccatgcg
    16021 tttattcgca tgttgcgcag tattcgtgaa gatgtcccca tcatcgccat gagcggtctt
    16081 ccggaaaatg aaaagttcat tcaaaaaaac ggattcaaag tacaggcttt tttgatgaaa
    16141 ccataccgcg ctgaaaaaat acttcaaacg ttgaacgacg tactcgcacc gttgcgttcc
    16201 gctgagcagt cctaattttc atattaacgg aaaggttttt ttaaatgacg atattgattg
    16261 tggacgatga aagaacttcc gtagacgctt taagtttggc tttggggcaa tccggttatt
    16321 cggtagtgac agcgttaaac ggcaaagcag cgcttacgat cctggccggt caatcaccgg
    16381 atttgatcat atcggacatt atgatgccgg aattagacgg attcggtttt atagaggctg
    16441 ttcgtagaca gccacgcctg gcgtccatac cgtttctgct gatgtcgggc gacaaagatc
    16501 cgtcattgac ggccaaagcc atccaaatgg gggccattga ttttttacca aaaccgatca
    16561 atctgaattt gctgtttttg aaaattcagg cgctgtttcg atttgtggaa gcccagcggt
    16621 ccagcggcat cctgcgaagc ggttcgctcg tgctcggaaa aatcgaagaa ctcatcgcgt
    16681 cagcggaaaa agaagggatt cacgggattt tgactatcca aagcgatcat ggcgtcggct
    16741 cgatcgagtt taatcgcggt gtatgcgaaa cgatcgtatg cggtgaatta aaagactcgg
    16801 aagcgctgga gacgatgtta cagtggagca gcggttcata ctatctcgca cgacgcccca
    16861 ctgaattaac cgaaagcgaa aacaaaatac cggtatcggt gcactcaacg aaggaaggaa
    16921 acgcgatgaa aagtgaggat ttttctaaac ctatagcgat tgccgaagat atttattttg
    16981 tcggcgacag aaaagaatcg cgtattcttc agattaaccc atttttgcgt gtgtataata
    17041 ataaggtcaa ttttttggtt gatcccggac cgacgattga ctatacgacg atttccggca
    17101 aagtcggcga ggtgatcggt gatgtgtcca aggtcaactt atacagcctc aatcatcagg
    17161 atcccgatgt agcttccaat acgtttttcc tcaagtcggc caaccctaaa gccattt
//
LOCUS       DAOQGF010000071        17150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1537_length_17150_cov_6.287628, whole genome shotgun sequence.
ACCESSION   DAOQGF010000071 DAOQGF010000000
VERSION     DAOQGF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_17150_cov_6.287628"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /gene="feoB"
                     /locus_tag="PK937_10350"
     CDS             complement(<1..1691)
                     /gene="feoB"
                     /locus_tag="PK937_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNH33015.1"
                     /translation="MSIVKENNKKQKSIQTPVFLNMSKTAHEMVIALAGNPNTGKSTV
                     FNALTGLNQHTGNWPGKTVKRMEGQFTYGERRYRLVDLPGTYSLLSASTDEEIARNFV
                     LFGKPDVTVVVVDATNLERNLNLALQIMQITDRVIVCVNLIDEAERKGIRIDAPRLQE
                     DLGIPVVLTAARSGQGLDILQETIAQLSKNLIPVKPFRINYPESLRETVDILSQKIHA
                     YDPKIPNPSWMAVRLLDKDQGIREALLSGHWHTRLLSNDIPVPALTNPDHPGVYEIRS
                     IIDEADSASEKIQTVLHDSLVESLFYDAKRIAGRAVEQTRITRLWENALDRWLTSRFV
                     GIPLMLLVLAVVFYLTIAGANVPSAMLADLLFSVEDFLAAGMNQLGAPWWLTGFLIHG
                     VFRGLAWVVSVMLPPMAIFFPMFTILEDFGYLPRVAFSLDRLFKWSGAHGKQALAMGM
                     GFGCNAAGVIATRIIDSPRERLIAILTNNFVPCNGRWPTLIMLASLFVAASLPAGVAS
                     IVAASAIVGITLFGILVTLLVAKFLSTATSLKGESSFFQIELPPYRRPQFFRILYT"
     gene            complement(1696..1944)
                     /locus_tag="PK937_10355"
     CDS             complement(1696..1944)
                     /locus_tag="PK937_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNH33016.1"
                     /translation="MKDHTTTPKITLDSVPIGASVRIAEMHIHGMARRRLLDFGFLRD
                     EPIEVVSRSPFGDPTAYRVKGTLIALRREEAKKIEVVL"
     gene            complement(1941..>2618)
                     /locus_tag="PK937_10360"
     CDS             complement(1941..>2618)
                     /locus_tag="PK937_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNH33017.1"
                     /translation="FLLVMKNKSTEDYLKHIFWLSRMRDKITTKAIAERMGISSASVT
                     DMIKKLTHHGYLDYTPYYGVRLTTKGDKLALKVIRRHRLLEAFLVKALGYSWEAVHDE
                     AERLEHVISEEMEHRLDEFLGHPRYDPHGDPIPTHEGKMEEIHHPSLAELSVGDTARI
                     MQVRDSEELLKYMKRIRLSLNSRIRICAKESYDNSMRLRIDGKEEHTLSHDVTSNIFV
                     KKEKEKK"
     assembly_gap    2619..2718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2884..3519)
                     /locus_tag="PK937_10365"
     CDS             complement(2884..3519)
                     /locus_tag="PK937_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH33018.1"
                     /translation="MSRLRTLLAEKFPDLQHKTLIAEMEKHSRLMTANPGDMLVKTND
                     YVKVVPLVTKGSIKVSRDDGEGHALFLYHIEPGQSCAMSLKSCLGEERSRIKGEADSV
                     TELIAIPSSFIEPWMTEFPAFKRFVINTFSQRYDELLSMIDTIAFEKMDRRIMTCLRQ
                     HARAGDGRTVRLTHQKVADELNTSREVVSRLLKVMERNGQVRLGRTTIEIL"
     gene            complement(3561..3992)
                     /locus_tag="PK937_10370"
     CDS             complement(3561..3992)
                     /locus_tag="PK937_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNH33019.1"
                     /translation="MPYDERLVAPMRAELTKIGFTELKTKAAVDEALQNQGTTLVVVN
                     SVCGCAAGMARPGVAMSLNNAKRPDHLTTVFAGQDTEAVARVREHIKEFPPSSPSAAL
                     FRNGKLVWMLQRFEIEGQTAMAVANKLAAAYEKHCVPATQG"
     gene            complement(4160..4450)
                     /locus_tag="PK937_10375"
     CDS             complement(4160..4450)
                     /locus_tag="PK937_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33020.1"
                     /translation="MCAANSTDVHIPLKGCRLDRYLCIKTERAVAKDYTIAHHGKLYQ
                     LHTRLPLKRVVVEERCDGTMYVVSGDHFVKHTEITHRPMKNFSIYNQPSGWL"
     gene            complement(4552..5232)
                     /locus_tag="PK937_10380"
     CDS             complement(4552..5232)
                     /locus_tag="PK937_10380"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HNH33021.1"
                     /translation="MSDRQVRRLAKAVRSRGEMGIVHGLRGKRSNRKIPEGVKAKVLS
                     QYQKRYLGFGPTLACEKLLEVDHLVVSDETLRPWLMEAGLHEKQRRTRVHRSWRRRRE
                     CLGEMIQIDGSHHAWLEDQGEELVLMGYIDDATGEVFGRFYDYEGTLPAMDSFKRYVQ
                     QYGLPMSVYLDRHTTYKSPKKLPEGVEDQEQPLSQFECALKELGVEVIHAYSPQAKGR
                     VERLFGVL"
     gene            complement(5271..5579)
                     /locus_tag="PK937_10385"
     CDS             complement(5271..5579)
                     /locus_tag="PK937_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33022.1"
                     /translation="MDAQRFGRNNWRLMGGVDYRASSYYVQSEAVHTKSRYDRIGGRL
                     SYFGELQPYNHTELYGKIGIVNMPGASQRYWMMYVSLNYRLNPLDVIAMTKKIILPGQ
                     "
     gene            complement(5661..>6185)
                     /locus_tag="PK937_10390"
     CDS             complement(5661..>6185)
                     /locus_tag="PK937_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33023.1"
                     /translation="ALYLKNLFRVELIADDYESNRLCDEKWTMIRHQPVRIKAYGPAS
                     WMIATIEPSKNIGGDGQVTRVNSRNTAQNKVNFEGTLCDNRFGDKGGKFLFFISHGPM
                     RWLRYLKAGFVLNWKGDLERIDSLRGATVKEKLIDLYSENSESMLYFTFPVLSQLSRG
                     RSYVMDWLVIRPMV"
     assembly_gap    6186..6285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6286..7370)
                     /locus_tag="PK937_10395"
     CDS             complement(<6286..7370)
                     /locus_tag="PK937_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33024.1"
                     /translation="MRSIRPLLYLWMGSIGTILLGQNLQLGDIKKSVTAITENINTAD
                     QQFRMLDTAIDNLDYKACRNTLTSLRQTAWGEAPSSSLKSNIADLTRHTETAYQELAR
                     HALRDVVTKTDWIQLKEDYAEIRDSVKMIITKLRDMQQKIAGIGQTAQAKIKEKKELE
                     EKALEFITALVMKRTASTRDSLYQVLVAQLERLGLTIEDVRFLSRWDQLEKNARYDSL
                     IVLTGKLADHWLRQETAEVFAEVLRMASDLQDKKITLDEVYTLANNRMIEKLEEEIGG
                     TALAKAAADEFSVPKVEALQVTLNHPEEVFLQPISGEQRYILYKEKIYRYEKQKTEYT
                     YIQAFDAKLENFELRFQPVIVKVRING"
     gene            complement(7712..7963)
                     /locus_tag="PK937_10400"
     CDS             complement(7712..7963)
                     /locus_tag="PK937_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HNH33025.1"
                     /translation="MKQFPSPASEPTNKAAYETQRTRFITGMVCTRAGVYIFESYADH
                     ATDSKPTDDELYVVLKYGQVFPPIRSVCKSAWWKLEKEK"
     gene            complement(8141..8707)
                     /locus_tag="PK937_10405"
     CDS             complement(8141..8707)
                     /locus_tag="PK937_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNH33026.1"
                     /translation="MLTNEQEKTLFGIPLRLDDIYPSVPPPINPPYELDEIMLTFGDI
                     FNYVNQDGSIKRSWEEGQMGFADIPFTIPFSSYPDIKVGKIYCHKKLISTFTKVFRVI
                     YEKNLHRHIHSYGGCYVYRNKNNQTQLSTHCWGIAIDLNPHTNAPGTRGDMNSDIVRV
                     FQSHGFEWGGNWKKKYRNPMHFQWCSGY"
     gene            8880..9494
                     /locus_tag="PK937_10410"
     CDS             8880..9494
                     /locus_tag="PK937_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH33027.1"
                     /translation="MLFAKTRYKDLSDFELIEAYRKSMNDRYLNELFSRYAYLVLFVC
                     NKYFSNPEDSRDATMEVLEKLAQGIVKSDVKNFKSWLYVVTKNLCLNKLQKSKDLDFQ
                     FVPIDGDETNYFMYFSEIDSLIDEDDDHSLKMKQALKEAIETLDPHQRECLMLFYYEK
                     KSYNEIMEMKNWDKDQVRSYLQNGRRNIKIYLKKSGLYVENIEN"
     gene            9472..10263
                     /locus_tag="PK937_10415"
     CDS             9472..10263
                     /locus_tag="PK937_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33028.1"
                     /translation="MSKISKTESESGGLKLTRSHQHCLTEEMIVNYLFRSLSDEERHR
                     IEEHITDCDLCYDAVSGSRRLGSERELTKQTMILRSEIEARYGRTHRSNKPAKFKYYA
                     AAALLLISSSSIGYWLTRHSHETIADAFLKPYSNTIPLTRGTDAQSLLEEAMAAYEAE
                     TYDASAQKLEDFLKTNPDHSVAHFYCGISLLLEGQFEESDLHFQKSEKSEDRRIAEAA
                     RWYRALAYVKMEKTDEAMNLLKEVAQRNGYYAPSAASLMERLQKE"
     gene            10260..11045
                     /locus_tag="PK937_10420"
     CDS             10260..11045
                     /locus_tag="PK937_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH33029.1"
                     /translation="MRILRVYILASAVLLGTLFAQEEEEKSARDTLRLRDLPWAEKEL
                     QSIASVVKSELYLRDDATEAAFKKNAGRFGIIHLAQHVILDDKEPLYTKFIFSDDPGS
                     GEDGYLHLYELYNLRLRANMVVLSACQTGIGKNIKGEGMMSLARGFTYSGCPSVVMSL
                     WSIDDKATSVIITDFYKKLAQGKTKDVALRDAKLNMIQSGDPVLSNPYYWSGLVVIGS
                     TESLEIPPPENFISYRIYATVVIVSILVLLILIGKKRMKSVSH"
     gene            complement(11042..11257)
                     /locus_tag="PK937_10425"
     CDS             complement(11042..11257)
                     /locus_tag="PK937_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33030.1"
                     /translation="MKKTKLAIVMYVILLTLAVSACGGPEGVVSSSTGKNSESTTGDK
                     KTYDDGSDDVFPQDTPIDPLPGGGGGN"
     gene            complement(11375..13834)
                     /locus_tag="PK937_10430"
     CDS             complement(11375..13834)
                     /locus_tag="PK937_10430"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33031.1"
                     /translation="MKITISRKLLLVLATGVTVLAGYFYVDMRREQNFLRSNEPVLSD
                     TTQARWWFEEGVSLTEKSRYDSSLIFFERATGIYQRDSLWQFYIDGMNYIGDNFRRLG
                     RYDTAFSILTANIDRSIRYLGETDVSTAMAINKMGLWYMDQGDDEKAKNNFNRALWIR
                     KKTLPGDHIDIGWSYNNLGLVNYNSGYFTLALQYYDQALGVFMRTIGENSGPVAVLNN
                     NMASVYQAQGNHEKALEHINRSLQIRKTLFGSDHVTLSENYTLMGTAYLRKGEMGQAF
                     KLYNQALSVNERKYGLGHPTVGNNYYNLGFAFKEVRQMDTALSYYRYALNNWKKNYGL
                     KHPVIARVSDEIGELFLEKNMYDSALFYFNNALTIWNNRPGAKGFRRAHEYSHIGMTY
                     LKMGDVKKSLTYYEQALSINLEYYGSKHLEVSLCYSNMAGAHRIVKDYPAALSNIQKA
                     IDALLIADSASGFMYRTRDAVKLMLALELKGDILQEYYEYKTRNLQHLKASFASYAMA
                     VEWLDRTRREYVSEDSKILIGQRSIGLFEKAIQTATDLYKITSEKKYLNEAFIYSETS
                     KAAVLSEVVSRQHAKRFAGIPDSLLEYEEQLRNRIGVYNKKLFSEQNSGGYGSAKFRY
                     IQDELFQLTSEYEAYLKHVEQTFPQLYSLKYKPLIMPLEAVQKLIPDSTVLLEYFIGN
                     RHLYVFCVTRSGCAWAAAPVDSSLIGVVHALTAGIQTMNMALYSTSAHYLYNVVFKPF
                     EKTSAVKLIVVPDGILSGIPFEVLLPRLPDNQEDYATLNYLIKKYHISYAYSASLLYQ
                     PSSVLRDGLNDLAGFTSTTFK"
     gene            complement(14020..14496)
                     /locus_tag="PK937_10435"
     CDS             complement(14020..14496)
                     /locus_tag="PK937_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH33032.1"
                     /translation="MKKWVMQWLVVVILCGPVIFAQDKPKIAVVPFKNNTGVKDYDGI
                     AVGIGESITTVLSGSGKFDFIERIQMDKAIQEQDFQMTDHVDPKTVQEIGKILGVQYM
                     IIGDLQKIKDKFRINARRMRVEDGKLEEAVSVTGIEDELFDMQDAIARKLDERFSP"
     gene            complement(14517..16616)
                     /locus_tag="PK937_10440"
     CDS             complement(14517..16616)
                     /locus_tag="PK937_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH33033.1"
                     /translation="MNSRISYRICFDRIQKWLLLVLTSLSLEIVLYAQAPVSRGVAVL
                     TFDNLTGDASNDWIGAGFGETMTNKLNNVNALRMQGRRQLNDLLLTRGWDQKKIRENQ
                     AEEVGRLLGVDYLLIGSVQAAADISNPEAELYVNARIVDVHTAQILQTLAIRGKMMNL
                     LGLQTALAMEFIRKLGVETTVSEQNALAYEETKSLEAYRYYAMGNDFLDQTRYKEAIE
                     MYKLAMAKHGGIFYAEANHNKVEAYLRLAAQSGDARDSVLTQLVHGEKKKVSELAPIF
                     FNLAQAEELNGNFDKAAEAYRTFIDYCDSKLLRWKFTSGKAIVAAPKTYQENMVFTAS
                     DNYVYNVQSVSGQLKWKNKISDKPQSEPFIFEGKIYLADEDENLFAVHALHGQLIWTK
                     HISGLTKAAPWVSDGLVVVGLRTGHVAAFRSAGGDPVWKTDIASPILGSTAGRDAKIY
                     VGTQDGTIACLDQKTGRLLWKTRLEGTIPTKVSPDKTMLFAVTEQGFVVALDPVSGQP
                     VWKTNVDGKVTQPAAWSDKDVFVVTEKGTIVALNKADGVIRWTYQSRAKQLTAPQWIE
                     QLVYIGADDHNVYAIHSQTGKLAWRYETNAEIASEPMILSGVVYVGTGDGGVYAISEK
                     KDPAQHSDLDAYLSLGRVATYQRKIEDALRIYEYITQYVKVNVPEAYIEMEKLYREKG
                     DEAKAKEMKKKYIQSRN"
     gene            complement(16662..>17150)
                     /locus_tag="PK937_10445"
     CDS             complement(16662..>17150)
                     /locus_tag="PK937_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33034.1"
                     /translation="HEKAYLAKKGSSIFLKIIFGTTTDNEVYELKNEAVLYTKVSHSA
                     GPWGDTYNEKGNPAIDSVRSTEYSFNYGGKFQYNSWVKANGYGNAETTPFTAYQGQQI
                     KWRLDFLTPYYDGWRNGNRGCYLNATGDLLYISAGDGLVHAVDLKKVDTLIGQGQKWW
                     KE"
BASE COUNT         4501 a         4143 c         3550 g         4756 t
ORIGIN      
        1 gtatataaaa tcctgaaaaa ctgcggacga cgatacggcg gtaactcaat ctgaaaaaat
       61 gaagattcgc ctttgagact ggtcgccgta ctcagaaatt tggccactaa aagggtaact
      121 aaaatcccaa acaaagtaat gcctacgatc gccgatgctg cgacaatcga agccacccca
      181 gctggtaacg aagccgctac aaaaagcgag gccagcataa tcaacgtcgg ccagcgtccg
      241 ttgcatggga caaaattatt ggtgagtata gcgatcaacc gctctctcgg tgagtcaatg
      301 atccgtgtcg cgatcacacc cgccgcattg cagccgaaac ccatacccat agccaacgcc
      361 tgtttgccat gtgctcccga ccatttgaat aaacgatcca aactaaacgc cacacgcggc
      421 agatatccga aatcttcgag tatggtaaac ataggaaaaa aaatcgccat cggtggtaac
      481 ataacagaca ctacccaagc caatccgcga aatacaccat gaatcaaaaa accggtcaac
      541 caccacggcg ctccaagttg attcataccg gccgctaaaa aatcttctac ggaaaacaac
      601 agatcggcca acatcgccga aggtacatta gctccggcaa tggtgagata aaacaccacg
      661 gccaatacca acagcatcaa cggaatacct acaaaacgcg aagtcaacca acgatctaag
      721 gcgttttccc aaagccgcgt tattcgtgtt tgttccaccg cgcgtccggc aattcgtttt
      781 gcatcataaa atagcgactc gacaagactg tcgtgcagta cggtttggat tttttcagat
      841 gcgctatccg cttcgtcaat gatacttctg atttcatata cgcccggatg atcagggttt
      901 gtaagcgcag ggacaggtat atcgttactc agaagacgcg tatgccaatg gccggataat
      961 aaagcttcgc ggataccctg atctttgtct aacaaacgaa cggccatcca ggagggattt
     1021 ggaattttcg gatcatacgc atgaattttc tgactcaaaa tatccaccgt ctctctcaac
     1081 gattccggat aatttattcg aaacggtttc accggtatga ggtttttact cagttgagca
     1141 atggtttcct gtaaaatatc gagcccttga ccgcttcgtg ccgccgtgag caccaccggt
     1201 atccctaaat cttcctgtaa gcgcggggca tcaatgcgaa tacctttacg ttcagcttcg
     1261 tcaatcagat tgacacatac gatgacgcga tccgtgattt gcatgatttg cagcgccaga
     1321 ttgagattgc gctccagatt ggtagcatcc acgacgacga cggtaacatc cggtttaccg
     1381 aataacacaa aattgcgcgc gatctcttcg tccgtcgaag ccgacagcaa tgaatacgta
     1441 ccgggcaagt ctaccaagcg gtaacggcgt tcaccatagg taaattgacc ttccatacgc
     1501 ttgaccgttt ttccgggcca attaccggta tgctgattta gcccggtcaa tgcattaaaa
     1561 accgtactct ttccggtatt gggattgccg gccaacgcaa tgaccatctc atgcgccgtc
     1621 tttgacatat taagaaaaac gggagtctga atgcttttct gttttttatt attttcttta
     1681 acaatgctca tagtcttata acacaacctc tattttttta gcttcttccc gacgtaacgc
     1741 gatcagcgtg cctttgacac ggtacgccgt cggatcacca aaagggcttc ggctcacgac
     1801 ctcaataggc tcatcacgca aaaaaccaaa atccagaagc ctacgacgtg ccataccatg
     1861 tatatgcatt tcggcaatac gtaccgacgc gccgatcggg acgctgtcca gtgtgatttt
     1921 gggggttgtc gtgtgatctt tcattttttt tctttttctt ttttaacaaa aatattgctg
     1981 gtcacatcgt gactcaacgt atgttcttct ttcccgtcaa tacgcagacg cattgagtta
     2041 tcataacttt ctttggcaca aatacgaatg cgcgaattca acgacaggcg tatccgcttc
     2101 atgtatttca acaattcttc ggaatcacgt acttgcatga tacgcgccgt gtcaccgacg
     2161 gaaagctcgg ccagtgacgg gtgatgtatt tcttccatct tgccttcatg ggtcgggatc
     2221 ggatctccgt gcgggtcgta acgcggatga ccgagaaatt catccagacg atgttccatt
     2281 tcctctgaaa tgacatgttc aagccgttcc gcctcatcat gtacggcttc ccaactataa
     2341 cctaatgcct tcacaagaaa ggcttctaga agacgatggc gacgaatcac tttaagtgca
     2401 agtttgtcgc ccttagttgt caaacggact ccgtagtacg gtgtataatc caaataaccg
     2461 tgatgcgtca gttttttgat catatccgtc acggacgcgg acgaaatacc catccgttcg
     2521 gcgatggctt tggtcgttat tttgtcccgc atacgactca gccaaaatat gtgctttaag
     2581 tagtcttccg tacttttatt cttcataacc aataaaaann nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnat aatagcgatg gccgcaatga ttcgcagcgc acgatctgca
     2761 tttcccatat tctttgtcat gacactctcc taagttaacg gttaagggac ttttaatgac
     2821 acatcacaag atagggcatg tttgtaccca tgtcagtaac atttgtcacc aagaacaaaa
     2881 aaattagaga atttcgatcg tcgtgcggcc gaggcgtacc tgaccattgc gctccattac
     2941 tttgagcaat ctggaaacaa cttcgcggga ggtattaagt tcatccgcta ctttctggtg
     3001 ggttaaacgc actgtgcggc cgtctccggc gcgtgcatgt tggcgaaggc atgtcataat
     3061 acggcgatcc attttttcga aggcaatcgt atcaatcatg gacaacaatt catcataccg
     3121 ttgactaaag gtattgatga caaaccgttt gaatgcggga aactccgtca tccagggttc
     3181 aataaacgac gaagggatag caatcaattc cgtcacagaa tcggcttcgc ctttgatacg
     3241 actgcgctct tcgccgagac aacttttgag agacatcgcg caggattgac cgggctcaat
     3301 gtggtataaa aaaagtgcat gaccttcgcc gtcatcacga ctcaccttga tcgaaccttt
     3361 ggtcactaac gggacgactt tgacatagtc gttagttttt acgagcatgt ccccgggatt
     3421 ggccgtcatc aagcggctat gcttttccat ctctgcgatc agcgttttat gttgcaaatc
     3481 cggaaatttt tcagcaagca atgtgcgtag ccgactcata acataatact accggtttat
     3541 tatcatttta aggattcgac tcacccttgg gtagcaggaa cgcaatgctt ttcataagct
     3601 gcggcgagtt tattggcgac agccattgcc gtttgtcctt cgatctcaaa tcgttgaagc
     3661 atccatacta attttccgtt tctaaacagc gcggccgaag gcgatgaagg cggaaattct
     3721 ttgatatgct cacgtacccg tgcgacggcc tcggtatcct gacctgcgaa aacggtggtt
     3781 aaatgatcgg ggcgctttgc attattcagc gacatcgcga caccggggcg agccattcct
     3841 gccgcacatc cgcaaaccga attgacaaca accaatgttg tgccctgatt ttgcagcgct
     3901 tcgtcaacgg ccgccttcgt tttcaattcc gtaaaaccga ttttggtcaa ttccgcacgc
     3961 atgggtgcaa caaggcgttc atcgtaaggc atacgttctc ctattatttc atttgtaaag
     4021 tttcaaaatt tggatttaat gctatggacg cgttactttc aatttatttt aataatattt
     4081 catttcactg catttgtcaa taaggtataa ttatatgtca agccaaaata gaaatgtccg
     4141 gtttttgatt gataaatggt tacaaccaac ccgaaggttg gttgtaaatc gagaaatttt
     4201 tcataggccg atgcgtgatt tctgtatgct tcacaaaatg atctccgctc accacataca
     4261 tagttccatc gcatcgttct tccactacaa cccgtttgag cggaagccgc gtgtgaagct
     4321 gatataactt gccgtggtgc gctatcgtat agtccttggc aacggcccgt tcggttttga
     4381 tgcaaagata acgatccaga cggcaccctt ttagcgggat gtgtacatcc gtggagttag
     4441 ccgcacacac cccaaattgt ttattgtagc gcggccaata ggtctctaaa aacgcattcg
     4501 ctccttcttt gtcgtcattc cggctagacg catttccttt accagacgat cctacagtac
     4561 accaaataat cgttcaacgc gacccttggc ttgcggagaa taggcatgaa tgacctccac
     4621 acccaattcc ttcagagcgc actcgaattg actaagcggc tgttcctgat cttcaacacc
     4681 ttccgggagt ttcttgggag acttgtaggt cgtgtgtcga tccagataca cactcatcgg
     4741 taacccgtat tgctgtacat aacgcttaaa actgtccatg gcgggcaagg tgccttcata
     4801 atcataaaac cgcccaaaca cctcgcctgt cgcatcgtca atgtaaccca ttaggaccaa
     4861 ttcctcgcct tgatcctcca gccaggcatg atgcgaacca tcaatctgga tcatttctcc
     4921 taaacattcc ctgcgtcgac gccatgatct gtgcacacga gtgcgtcgtt gcttttcatg
     4981 tagtcccgct tccattaacc atggccggag cgtttcatca ctgactacca agtgatcaac
     5041 ctccagtagc ttctcacacg ccagcgtcgg accaaaaccc aaataccgct tctggtattg
     5101 gctcagcacc ttggccttaa ctccttcggg aatctttcga ttcgaccgct taccacgcaa
     5161 tccatgtact attcccatct ccccgcgcga tctcaccgct ttcgcaagac gtctgacttg
     5221 gcgatcactc aaagcaagca tcgcaccgct tataacttcc catagtatcg ttattgtccc
     5281 ggcaaaatga tcttcttcgt catcgcgatc acatcgagcg gattgaggcg gtagttgagc
     5341 gaaacgtaca tcatccagta cctttgggaa gcgccgggca tattgacgat acctatcttg
     5401 ccgtacagct ctgtatgatt atacggctgg agttcaccaa aataactcag ccgaccgccg
     5461 atcctgtcat atcgcgactt ggtgtgaacc gcttccgact gaacatagta ggatgatgcc
     5521 cggtagtcca caccgcccat gagtcgccag ttattacggc caaagcgttg tgcatccaaa
     5581 aggcgtactt gtaatccact cgacagctta tcgtacgcac ggtatgtgcc ggcagcatcc
     5641 tgtactttaa atcgttcgtg ttacaccatc ggacggatca ccagccaatc catcacatag
     5701 gaacgtccgc gcgaaagctg actgagaacc ggaaaggtaa agtacagcat ggactcggaa
     5761 ttttcggaat agaggtcaat gagtttttct ttgaccgtcg ccccgcgtag cgaatcaatc
     5821 cgctccagat cgcctttcca gttgagcaca aatccggctt tgagataacg taaccatcgc
     5881 atgggcccgt gcgatataaa aaacaagaac ttcccgcctt tatcgccaaa gcgattatcg
     5941 caaagtgtac cttcaaaatt gactttgttc tgtgccgtat tgcgcgaatt gacgcgcgta
     6001 acttgtccgt caccaccgat gtttttggac ggttcgatgg tagcgatcat ccatgaagcc
     6061 ggcccgtacg ctttgatccg taccggctga tggcgaatca tagtccattt ttcatcgcat
     6121 aagcggttgc tttcatagtc atcagcgatg agttcgaccc gaaaaaggtt tttgagatac
     6181 aatgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgcc attaattcta
     6301 accttcacga tcaccggctg aaatcgcagt tcaaagtttt ccagtttggc atcaaaggct
     6361 tgtatgtacg tgtactcggt tttttgtttt tcataccgat aaattttttc tttatataag
     6421 atataacgtt gttctcctga tatgggttgt aaaaacactt cctcgggatg attgagcgtg
     6481 acctgcagag cctccacttt cggtacggaa aactcgtcgg ccgcggcttt ggcaagcgcc
     6541 gtaccgccga tctcttcttc caatttttcg atcatgcggt tattggccaa cgtatagact
     6601 tcgtccaaag taattttttt atcctgaaga tcagacgcca tacgcaggac ttcggcaaaa
     6661 acttccgccg tttcctgccg cagccaatga tccgcaagtt tgcccgtaag cacgatcagc
     6721 gaatcataac gcgcattttt ttccaattga tcccagcgtg acaaaaatcg tacatcctcg
     6781 atcgtcaagc cgagcctctc aagttgtgcc actaatactt gatataatga atcccgcgtt
     6841 gatgcggtgc gcttcataac aagcgccgtt ataaattcca acgctttttc ctcaagttcc
     6901 tttttttctt ttattttggc ctgcgcggtc tggccgatac cggcgatttt ttgttgcata
     6961 tcgcgcagtt tggtgatgat catttttacc gaatcgcgga tctccgcata atcctccttg
     7021 agttgtatcc aatccgtttt ggttaccaca tcgcgcagag cgtggcgtgc cagttcctga
     7081 tatgccgttt cggtatggcg cgtcagatcg gcgatattgc ttttgagcga tgaggatggc
     7141 gcctcgcccc atgccgtttg acgcagcgat gtgagtgtat tgcggcaagc cttataatct
     7201 aaattgtcaa tagctgtatc aagcatacga aattgttgat ccgccgtatt gatgttttcc
     7261 gtgatcgctg tgacgctttt tttgatgtct cccagttgga gattttgacc aaggaggatg
     7321 gtaccgatag accccatcca aaggtaaagc agaggacgga tactacgcat acgatggccc
     7381 tttctatatg tatgtgtaat acgaaaaacc gacggaataa acttcagacg gtgggcacac
     7441 ggtcgctgta cctgtcccga acaaaagaga ttcacaagaa atttttaccg gtaatgcatc
     7501 gaagcatcgt accgctttgc atgaccgcgc caatgcatcc ggtcgtccgg ttatcggcat
     7561 ccctggaaat gaagttgatt atattttgtg ttcgaaaatt tgtttctatt tagcgatagc
     7621 tgagacaagc gataccaatt cttccgtttt taaatttcac aaaacgtata cccctccgtt
     7681 tcatcacggc atgcgattaa ttcatcgcag tttacttctc cttctccagt ttccaccacg
     7741 ccgatttgca caccgaacgg atgggaggaa aaacctggcc atatttaagt acgacataca
     7801 attcatcgtc tgtgggtttt gaatcggtag catgatcggc ataggattca aaaatataaa
     7861 ccccggcgcg cgtgcaaacc atacccgtga taaaacgcgt gcgttgcgtt tcatatgccg
     7921 ccttgtttgt cggctccgat gcgggagaag gaaattgttt cattttacac aaatgctatg
     7981 tttgaaggtt ttgttacact tttttgaatc atgctatgat gtctcatgga ccccagcacg
     8041 gaggcccggt acggcaatta gaagtgggag gggttttctt ttattgtgta ccggcctcgg
     8101 ctgatattaa cgatacaaag agacccaaaa ttcatatttg ttaatacccg gagcaccatt
     8161 ggaaatgcat cgggtttcta tatttttttt tccaattgcc gccccattcg aaaccatgcg
     8221 attgaaatac acgaacgatg tcggaattca tatctcctct tgtccccggc gcattcgtat
     8281 gaggattgag atcaatagct ataccccagc agtgcgtgga cagttgggtt tgattatttt
     8341 tattacggta cacataacag ccgccatacg agtgaatatg ccgatgcaaa tttttctcat
     8401 atatgacccg gaatacttta gtaaaagttg aaattagttt tttgtgacag tatattttgc
     8461 ccacttttat atccggataa cttgagaacg gaatggtaaa tggaatatcc gcaaatccca
     8521 tctgtccctc ttcccaactg cgtttgatcg aaccgtcttg attgacataa ttaaaaatat
     8581 caccgaacgt cagcatgatc tcatccaatt cataaggtgg gtttatcggc ggcggcactg
     8641 acgggtagat atcatccagt cgaagcggaa tcccaaacag cgttttttct tgttcattag
     8701 tgagcatcga gttctttgcc ttggttccat tatagcttat tgcaataaga cagcattttt
     8761 gaaaaaaatg cataaactaa gtttttaacc ttgatatttt atcaaacgca ttaaaatcaa
     8821 tctcttaaaa tgaggttttg cattttgaaa tacggatttt aatcatcgga atgccattta
     8881 tgctgtttgc aaaaacaaga tataaagatt tatcagattt cgaactcatc gaagcctatc
     8941 gaaaatctat gaacgatcgg tatcttaacg aactatttag tcgttatgcg tatctcgttt
     9001 tatttgtttg taataaatat ttcagtaatc ccgaagactc cagagatgct acgatggagg
     9061 ttttggagaa actggcacaa gggatcgtca agtccgatgt gaagaatttt aagtcatggt
     9121 tgtatgtggt tacaaaaaat ctctgtctca ataaacttca aaaatctaaa gacttagact
     9181 tccagtttgt tcctatcgat ggcgatgaaa caaattattt tatgtatttt tctgagattg
     9241 acagtcttat tgatgaagac gatgatcata gtttgaaaat gaagcaggct ttaaaagaag
     9301 ccatagaaac cttggatccc catcagagag aatgtctgat gctcttctac tatgagaaaa
     9361 aaagctataa tgaaatcatg gaaatgaaaa attgggataa agaccaagtc cgtagctatt
     9421 tgcagaacgg acgaagaaac attaagatct atctaaagaa aagcgggctg tatgtcgaaa
     9481 atatcgaaaa ctgaatcaga atccgggggc ttaaaattga cgcgtagtca ccagcattgc
     9541 ctgacggaag aaatgatcgt caactatctg tttcgctccc tatcggacga agagcggcat
     9601 cgcatcgaag aacatattac cgactgcgat ctgtgctatg acgccgtaag cggttcgcgc
     9661 cgattgggtt ccgaaaggga attgacgaaa cagaccatga tactgcgatc ggaaatagaa
     9721 gcacgttatg gccgaaccca tagatccaat aaacccgcta agttcaaata ttatgccgcg
     9781 gcggctttac tattgatttc atcgagcagc atcggttact ggctgacacg ccattcccac
     9841 gaaaccattg ccgacgcgtt tctcaaacca tactccaata ccataccttt gacccgtggc
     9901 accgacgcgc aaagtttact ggaagaggcc atggccgctt atgaagcaga aacgtacgac
     9961 gccagcgctc agaaacttga ggatttcctc aaaaccaatc cggatcacag cgtggcccac
    10021 ttttattgcg gcataagcct gctcttggaa ggtcaatttg aagaatccga cttgcacttt
    10081 caaaaatcgg agaaatcgga agaccggcgc atcgcagaag cagcgcgttg gtaccgggct
    10141 ttggcctatg tgaaaatgga aaaaacagat gaggccatga acctgctaaa agaagtcgcc
    10201 caacgtaacg gatattacgc tccgtcggcg gcttccctca tggaacgcct ccaaaaagaa
    10261 tgagaatttt aagagtatat attcttgcat ccgccgtttt attgggaacc ctctttgctc
    10321 aggaggagga agagaaaagc gcccgggata cgctgaggtt acgcgatctg ccatgggcgg
    10381 aaaaagaatt acaaagcatt gcttccgtcg taaaaagtga actctacctg cgcgacgatg
    10441 ccaccgaggc cgctttcaaa aaaaatgccg gacgctttgg cattatccat ctcgcccaac
    10501 acgtaatcct tgatgataaa gaacctctct ataccaaatt tatcttttct gatgaccccg
    10561 gatcagggga agacgggtat ctccaccttt acgaattata caaccttcgg cttcgcgcca
    10621 atatggtcgt actgagtgca tgccaaacag gcatcggaaa aaatatcaaa ggcgaaggaa
    10681 tgatgagcct cgcgcgaggt tttacctaca gcggttgccc gagtgtcgtg atgagcttat
    10741 ggtctattga cgataaagcg acttccgtga tcatcactga tttttataaa aagctcgcgc
    10801 aaggcaaaac caaagatgtc gccctgcgcg atgctaaact aaatatgatc caaagcggtg
    10861 atcccgtttt atccaatcct tattattggt ccgggttggt cgtcatagga agtacggaat
    10921 cactagaaat tcctcctccc gaaaatttta tatcgtaccg gatttacgcc accgtcgtga
    10981 tcgtttcgat ccttgtactt ttgattctca tcggtaaaaa aagaatgaaa tccgtttcgc
    11041 attaatttcc gccaccgcct ccaggaagcg gatcaatcgg cgtatcttgc gggaaaacat
    11101 catcgctacc atcatcatat gttttcttgt cgccggttgt tgattccgaa ttctttcctg
    11161 tactgctgct gaccactcct tcaggcccac cgcaagccga aacagccaac gttaataaaa
    11221 taacgtacat cacaattgcc aatttagttt ttttcatgac actctcctta ggggttatta
    11281 gttatgtacc aaatataacc aataagacaa tcattcctga gaaagtgcat aaacttttac
    11341 gggcgggcaa tctgggacga gcaaaaaaat cgtgtcactt gaacgtggtg gacgtgaagc
    11401 cggctaaatc attaagccca tctcttaaga ccgaagacgg ctggtataat aatgaagcgg
    11461 aatacgcata actaatgtgg tattttttga tgagatagtt gagtgtagca tagtcttctt
    11521 gattatcggg taaccggggc agtaacactt caaacggaat acccgagaga ataccgtccg
    11581 gcacgacaat cagttttacg gcgcttgttt tttcgaatgg cttaaataca acattataca
    11641 aataatgcgc tgaagtcgaa tacaatgcca tgttcatcgt ttgaatcccg gcagtcaacg
    11701 catgcacgac tccaatcagc gatgaatcaa ccggcgccgc ggcccatgcg catcccgatc
    11761 gggttacgca aaacacatac agatgccgat ttcctataaa gtactccaat aagaccgtcg
    11821 agtcgggtat caatttttga acggcttcga gtggcatgat aagcggctta tatttgagag
    11881 aatataattg cggaaaagtt tgctccacat gcttgagata ggcttcatat tccgaggtca
    11941 attgaaataa ctcgtcctga atatatctga atttagcgct gccataacca cccgagtttt
    12001 gctcagaaaa aagtttttta ttatatacac cgatccggtt gcgcaattgt tcttcgtatt
    12061 ccagcagtga atcgggaatg ccggcaaatc gtttggcgtg ctggcgactc acgacttcgg
    12121 acaaaacagc cgctttgctt gtttccgaat aaatgaaggc ctcgttgagg tatttttttt
    12181 cagaggttat cttataaaga tccgttgccg tttgaatggc tttttcgaaa agccctatag
    12241 aacgctgacc tattaatatt ttagaatcct cggatacata ttcccgccgc gttcgatcta
    12301 accactctac ggccatggca tacgacgcaa acgacgcctt gaggtgttgt aggttccggg
    12361 ttttatactc ataatactcc tgcaaaatat cacctttcaa ttctaacgcc agcatgagtt
    12421 tgactgcatc gcgcgttcgg tacataaacc ccgatgccga atcggcgatc aaaagcgcgt
    12481 cgatagcttt ctgaatgttg ctcaaagccg caggatagtc ttttacaatc cggtgcgcac
    12541 cggccatatt ggaataacaa agggaaactt ctaaatgttt gctcccataa tattctaaat
    12601 taatagacaa tgcttgttcg taataggtca gacttttttt gacatcaccc attttcaaat
    12661 acgtcatacc gatatgactg tattcatgtg ctctgcgaaa acctttggca ccgggtctgt
    12721 tattccaaat agtaagcgcg ttattaaagt aaaaaagcgc gctgtcatac atgttttttt
    12781 ccagaaataa ttcgccgatt tcgtcggata ctctcgcgat aaccggatgc ttcagtccgt
    12841 aatttttttt ccaattattt aaagcataac gatagtagga caatgccgta tccatttgac
    12901 ggacttcttt gaaggcaaaa cctaaattgt agtaattgtt cccaaccgtc gggtgcccca
    12961 gaccatattt cctttcgttg acagataacg cctgattgta caatttgaac gcctgaccca
    13021 tctcaccttt tcgcaaatag gcggtcccca tcaacgtata attttccgat aacgtgacat
    13081 ggtcgcttcc aaaaagcgtt tttcttattt gtaatgatcg gtttatatgc tccaacgctt
    13141 tttcatgatt tccctgcgct tggtaaacgc tggccatatt attatttaat acggcgacgg
    13201 ggccgctgtt ttctccgatg gttctcataa aaacaccgag cgcctgatcg taatattgca
    13261 acgccaacgt aaaataaccg ctgttatagt tgacaagtcc caggttattg taactccatc
    13321 cgatatcaat atgatcgccg ggaagtgttt tttttcgtat ccataaggct ctgttgaaat
    13381 tgtttttggc tttttcgtca tcgccttgat ccatgtacca cagacccatt ttattgattg
    13441 ccatcgccgt tgatacatcg gtttccccca aataccgaat actccgatct atgttggccg
    13501 tgagaatgga gaaagccgta tcgtatcggc caaggcggcg aaaattatcg ccgatataat
    13561 tcataccatc aatataaaat tgccacaaac tgtcgcgttg atagataccg gtcgcacgct
    13621 caaaaaaaat caaggaactg tcgtacctac ttttttctgt caaagaaacg ccttcttcga
    13681 accaccatct tgcttgtgtg gtatccgata atacgggttc atttgaccgt aaaaaatttt
    13741 gctcacgccg catatcaaca taaaaataac ccgccaaaac ggttacgcct gttgccaata
    13801 caagaagaag cttacgcgaa atggtaattt tcatatatcc gatggattgg ataaaatagc
    13861 ggttaaacca gtggaaaaat ataggtgtga ttctatatat agaaaacggg ttaagcaata
    13921 tcagaattta tccctgtcgg cacgggcgat aaaatctcaa gtgtgtcact caacatcaat
    13981 ttccatcatc tctaatacgt gaggtatgtg attttatttc tacggactaa atcgttcatc
    14041 caactttcgc gcaatcgcat cctgcatatc aaaaagttca tcctcaatac cggtgacact
    14101 cacggcttcc tccagtttcc cgtcctctac acgcatgcgg cgagcattga tacgaaattt
    14161 atcttttatc ttctgcaaat cgccgatgat catgtattgt acaccgagga tttttccgat
    14221 ttcctggacc gttttgggat ctacatggtc agtcatctga aaatcctgct cctggatcgc
    14281 tttatccatc tggattcgtt caataaaatc aaatttgccg ctccccgata aaacggtcgt
    14341 gatactttca ccgatgccta ccgcgatacc gtcataatct ttaacacccg tattattttt
    14401 aaacggaaca acggcaattt ttggtttgtc ctgtgcaaaa atcaccggtc cacaaagtat
    14461 caccacaacg agccattgca tcacccattt tttcataaac cactctcgtt tgtatattag
    14521 tttcgtgatt ggatgtactt ctttttcatc tctttagctt tggcttcatc gcctttttca
    14581 cggtacagtt tttccatttc aatatatgct tccggcacat tgactttgac gtattgcgtg
    14641 atgtattcgt agatacgcag agcgtcttcg atttttcgct gataggtcgc cacgcgtccg
    14701 agcgacagat acgcatccaa atcactatgc tgcgcaggat cctttttttc actgatcgca
    14761 taaactccgc catcgccggt gcctacatat acgacgccac ttaaaatcat cggctcgctc
    14821 gctatctcag cattcgtttc atagcgccat gctaactttc cggtttgaga atgaattgca
    14881 tacacattgt gatcatccgc accgatataa acaagttgct cgatccactg cggagcggtt
    14941 aactgtttag ctcgcgattg gtacgtccat cgaatgacac catctgcttt attgagtgct
    15001 acgattgttc ctttttcagt cactacaaat acatctttat cactccatgc cgcgggttgt
    15061 gttacttttc catccacatt tgttttccaa acgggttgac cactaaccgg atcgagtgca
    15121 acgacaaacc cttgttccgt gacagcgaac agcatcgttt tatccgggct gactttagtc
    15181 ggtatcgtac cttctaatcg tgtcttccat aagagtcgcc cggttttttg atcaaggcaa
    15241 gcgatcgtac cgtcctgagt gcctacatat attttagcat ctcgtccggc cgtactgcca
    15301 agtatcggag aggcaatatc cgttttccaa accggatcac cacccgcgct tcgaaacgcg
    15361 gctacatgtc ctgtacgcaa gcctacaacg acaaggccgt cggataccca aggcgccgct
    15421 ttggtcaaac cggagatatg tttggtccat ataagttgac cgtgaagcgc atgaacggca
    15481 aaaagatttt catcttcatc ggctaaatag attttgcctt caaagataaa tggttcactt
    15541 tggggtttat cgctgatttt atttttccac tttagttgcc ccgacacgct ttgcacatta
    15601 tagacataat tatccgacgc tgtgaaaacc atgttttcct gataggtttt tggcgctgcg
    15661 acgatcgctt tcccggatgt aaatttccac cgaagaagtt tagaatcgca atagtcaatg
    15721 aatgtccggt aggcttccgc cgctttgtcg aaattgccgt tcagttcttc ggcctgcgcg
    15781 agattaaaaa atataggcgc cagttccgaa acttttttct tttcgccatg aaccaattgg
    15841 gtcaatacgg aatcccgtgc gtcgccactc tgcgccgcca ggcgaagata ggcttccact
    15901 ttgttgtgat ttgcttccgc ataaaagatg ccgccatgct tggccatcgc cagtttatac
    15961 atttcgatcg cttctttgta gcgtgtttga tcgagaaaat cattgcccat ggcataataa
    16021 cgatacgctt ccagcgattt agtttcttcg tacgccaaag cgttttgctc gctcaccgta
    16081 gtctcgacgc ccaactttcg gataaactcc attgccagcg ctgtctgtaa cccgagcaga
    16141 ttcatcattt ttccacggat cgctaacgtt tgaaggatct gcgccgtatg cacatccacg
    16201 atacgcgcat tgacatagag ttccgcttcc ggattgctga tatcggccgc ggcctggacc
    16261 gaaccgatca gcagataatc tacaccgagc aatcggccta cttcctccgc ctggttttcc
    16321 cggatttttt tctgatccca tccacgcgtc aaaagtaaat cattgagctg acgccggcct
    16381 tgcatacgaa gtgcattgac attattcagt ttattcgtca ttgtttcgcc aaaccctgcg
    16441 ccaatccaat cgttggacgc atcgccggtg aggttatcaa atgtcaatac ggcgactcca
    16501 cgagagaccg gggcctgagc atacaacact atctccaggc ttagactggt caaaaccaac
    16561 aacagccatt tttgtattcg atcaaagcag atcctataac ttatgcgact gttcataagt
    16621 tccttttcaa atttatcgtg catgacattt ttttcgccac attattcctt ccaccatttt
    16681 tgcccctggc cgatcaatgt gtccactttc ttaaggtcaa ccgcgtgtac caatccgtcg
    16741 cccgccgata tatacaaaag atcaccggtg gcattgaggt agcaaccgcg attcccattg
    16801 cgccaaccgt catagtaagg cgttaaaaaa tctaaacgcc acttgatctg ttgaccttga
    16861 taagcggtga aaggtgttgt ttcagcattt ccatacccgt ttgcttttac ccaactgttg
    16921 tattgaaatt tgccgccata gttaaaagag tattctgtag atcgaacaga gtctattgca
    16981 gggttacctt tttcattata ggtatctccc cacggtccgg cgctgtgact tactttggtg
    17041 tagagcacag cctcattttt caattcatac acttcattat ccgtggttgt gccaaagatg
    17101 attttaagaa aaatggacga acctttttta gccagatatg ctttttcatg
//
LOCUS       DAOQGF010000072        17072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1542_length_17072_cov_7.638420, whole genome shotgun sequence.
ACCESSION   DAOQGF010000072 DAOQGF010000000
VERSION     DAOQGF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_17072_cov_7.638420"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PK937_10450"
     CDS             <1..931
                     /locus_tag="PK937_10450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH33035.1"
                     /translation="ILCAAYAFNSLNTPVLTSIYRSNYPDHSRGKVMGIVRTVFNIAF
                     VVASYVFGLMLDLDGHNYVYVYPIIGLVGLWGGWQLRKIKIVPTVHTTPPMHPIRSAL
                     TILRQDKPFAHFMGYWAIFGFAMLMIDPVKTIYVTDPAYGINATYEEAVLAVTVIPQA
                     VMLLTFAFWGRQIDKYGVIKIRFLLNILPTINVILFYFATDLRLIYFASFLQGISMSG
                     AQLSWQLCVMEFAPRQQVGLYMGIHTMLTGLRGIVAPFIGAWLIGTSGMGNTFLFAAG
                     LMVISTVMMIRFSANHIRRDIEVPLPIAAERGD"
     gene            complement(1089..1985)
                     /locus_tag="PK937_10455"
     CDS             complement(1089..1985)
                     /locus_tag="PK937_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33036.1"
                     /translation="MFSVLCSCNDAPEGSPYLDKDYNYIVGILVPDSFRTYRQEIFVG
                     KMLDILVHSNEVDTAQYGEDPVLHIQNLVTWHDYRGKIVGTTGASVEVIDITDNESVT
                     FQDIGNGIYRDVNEQLHVHPLHRYSLKVTTTEKAFTAETTVPGDFQISNINEDDTLTV
                     VAIKRSYYYSANYPPYWTSSQGKFFYRTDHLTSQFFINFINHVYEPPGFFTVTPDTVN
                     YQLPFIFSGWIQVMALDTNYGRMYSPEETSTAPIDLLTYISNQETTPLPERTNIKGKE
                     AVGVFGSLNRTKRVNFYMKVVK"
     gene            complement(2053..2982)
                     /locus_tag="PK937_10460"
     CDS             complement(2053..2982)
                     /locus_tag="PK937_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33037.1"
                     /translation="MKYHVVVFLFFLAISCSCGDPEREDSPYLERDYVYITGILVPDS
                     FRAYRQEVFVGKMLDIKIGSEEVDTTHYGEDPLLYIQNLVTWHDYRGKLVGTSGATVF
                     IRDQYGTEVQLLDIGNGFYQDVANELKIKSMITYTLIVTIGNDTSEATTTVPGNFQLS
                     NVTDGDTVDVTVRPYRNTYGGDFFPYWSLSNSTFFYRTLRSTSLFPPIILKHIYSPPS
                     VIPLIIDTTEVSIDTVVASWIEVTSLDSNYGKMYSPEQTSTGPTDLLTYLSDQETTSL
                     SKRTNIKGRNVAGVFGSLNRTKRVNFYMKAVKQ"
     gene            complement(2982..3905)
                     /locus_tag="PK937_10465"
     CDS             complement(2982..3905)
                     /locus_tag="PK937_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33038.1"
                     /translation="MKQTIALVALFFAVSCSCNDAPEGSPYLEKDYNYITGILVPDSF
                     RTYRQEIFVGRMLDEKVGSGEVDINMYGEDTTLWLQNLVTWHDYRGKLVGTEGATVKI
                     KDQDNHEVVFQDIGKGFYRDVENALKIKALNHYELTVVTKGRTFTAETSVPGDFYVTN
                     LIEGDTIIETAQREEYFYWAEHKPSWTISSGTFYYRTDHHASAFEFNVINHVYSPPGF
                     FLVNADTLNHKLPLLYSGWFQVMAMDSNYGRMYSPEESSTAPIPLLTYLSTQETKPLP
                     SRTNIKGRDATGVFGSINRTKRISFYFKMVK"
     gene            complement(3976..5346)
                     /locus_tag="PK937_10470"
     CDS             complement(3976..5346)
                     /locus_tag="PK937_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33039.1"
                     /translation="MKLKFLATALFGFYLAAITINSCSDNEQPVHANDENAAFPQINI
                     SSRKELFETRGLAKRVANQGIKELSRFFAMDKKWLKKSVLQDTKKAGVPIDQLAVEAA
                     TSQITLDGVDYTIKVELLNSKQAFALDKKDKKFSYGVALDPDLFHADESDIPVYFWNE
                     DSKSIEEKTISLPMSSDTEERGEAMKAAKDAIEYPVFLISLEQTGDDPIIDAEFRALL
                     QKRIKEYADRVVKSNSSQLALLPPPPCEDCPVPPPPPPPPVPVTVPYFVVKQINLFDK
                     KDYTNDEFEMYVGESTENAGEIRGHTIHKFNGNTRSDAAGRSVYYRDVNGDQGWEIMS
                     NDIAIWPLQWIDPNGWMRVVDIENDDDAGKHDNVNDGSTISVDIKHYDVFAQSVKTNL
                     WKFYVAGGTLFSTDFDDIYGQSGIDKINVATVNAKLGSGYLNDYIKLEGRILNDIDYW
                     VGVRKY"
     gene            complement(6072..7568)
                     /locus_tag="PK937_10475"
     CDS             complement(6072..7568)
                     /locus_tag="PK937_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNH33040.1"
                     /translation="MSILHDNFLGPKAENAEALQESLIKILDWHAAWRRTFFTNDPEV
                     FDRSATPSDNTLQKHLDVLLERLSHQPPYFSPRYAAQMLKDPALSSTLGYLAAILTNP
                     NNHAYEGGPITTELEMECVQDLLNLCGFKKGWGHLCSGGSLANTEAMWAVRDVYTQKH
                     KKPGAVLFSHMAHYSWKRICSILAIQDGVEIPTDSKFRMDLDALESVLKKKKTMMVVA
                     NIGTTGVGAVDDIEGILQLRKRYGFHLHLDAAYGGYSRSIIIDASGRIKPYANVALIV
                     KPEVYLSLRAMKDADSITIDPHKQGALGYGCGAVIYKSEALRSAILNTAPYTYHIKDK
                     PNIGMFTLEGSRPGAVAAGCWLTHRMMPLHEEGYGRLLTENFLAAQELHRRIESESGF
                     RPLHDPDLDIYSFYKPIAASKTASLQKQNKLNTSIYEAFSVLNPKAPFILSKFVVDAP
                     SVKHATGALKTDDRFFLSLRTVLIKHWMRMSKPSYVDLLIEALREKRI"
     gene            complement(7616..7837)
                     /locus_tag="PK937_10480"
     CDS             complement(7616..7837)
                     /locus_tag="PK937_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNH33041.1"
                     /translation="MPIYEFTCKKCGTDFSELFHSSTFDASEVECPKCRAHEANRKLS
                     VFAADCKSSGESMPMMGGGCGAGCGCHMN"
     gene            7953..9353
                     /gene="lpdA"
                     /locus_tag="PK937_10485"
     CDS             7953..9353
                     /gene="lpdA"
                     /locus_tag="PK937_10485"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNH33042.1"
                     /translation="METKNFDVVVLGGGPGGYVAAIKAAQLGFKTAVVEREKIGGVCV
                     NIGCIPTKALLKNAEMYEHMKHAAEWGYSFDNLKYDYKAIIKRSRDVADANSKGGDFL
                     MKKNKIEVIKGTGKFLDKSTLSVADATGKETTQVKGKHIIIATGARARMLPNLKADGK
                     RILTSTEAMTNTEQPKSIVIIGAGAIGIEFAYFFNTFGTKVTVVEMMPTILPVEDEEV
                     SNTLLKVLQKKGMDIHTDTKVEKVDVGANNVTVTVSSKGKTQTITADQCLVAIGVQGN
                     TENIGLEKIGVTVEKGWIKVDEYYRTNVQGVYAIGDIIGAPWLAHVASHEGIVCVEKM
                     AGKDTHPIDYSSIPGCTYCQPQVGSIGLTEKKAREKGYDIKVGKFPFSASGKARAIGE
                     RDGFVKVIFDAKYGELLGAHILGSEATEMIAEYGIAKSLESTYKEIGNTIHAHPTLSE
                     AMMEAALDAYKEAVHI"
     gene            9450..10031
                     /gene="yihA"
                     /locus_tag="PK937_10490"
     CDS             9450..10031
                     /gene="yihA"
                     /locus_tag="PK937_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNH33043.1"
                     /translation="MIIKSVEFIASVYKPEQVPKPQLPEIAFFGRSNVGKSTLLNTLT
                     GKKMARVSSTPGKTQCLNYYKINDAFYLIDAPGYGYAKVERKLREAWQRNLGAWLTEN
                     DRIRLAVLIIDARIEPQASDLQMAEFLNYYQVPTLIAANKADKLKNSERKPILKLLNE
                     AYGTWEWPIDFTSGETGEGKVAVLKAMDSALTH"
     gene            10089..11501
                     /locus_tag="PK937_10495"
     CDS             10089..11501
                     /locus_tag="PK937_10495"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNH33044.1"
                     /translation="MKHRILFVCCVMLWASMTQINAQKGSGNQSSGRLSFYVDYAAFK
                     MFNQKNVTYTQLYFYMSRNQFNFVKKDTSYSAAYTVSLEFTDLNDSKNVVQNTWTTVM
                     DDRIAEADTGKEVPLLFEYPLLLKPSKYILKATIQDKNDASRSGSYTESIEINDFDKS
                     DLMISQIQFATQALKGGNSGEMFVKNGYTVYPNPTRFYGSNLPRLSFYSEVYNLKHTP
                     DDATTNTYTAEFIVTDENGSIIKEYPSRQINKAGETSLLMHNVNVISLPSGRYVFMVR
                     ITDNASKSVAISKKLFVVYREGESIYETQADDSFFKDLDEAGAIRAGNIVSLIGREDE
                     KKIFSQLEFEGKKKFLDRFWKDRDPSPGTKSNEKLMDYYKRYEEANMKFTTPNVQGWK
                     TDMGRVMVVYGFPAQIEKHEFESDMKPYQIWYFLQLKDQPAQTIFVFADMDESGTLRL
                     IHSNARGEIASPNWQTMIKK"
     gene            11540..12844
                     /gene="asnS"
                     /locus_tag="PK937_10500"
     CDS             11540..12844
                     /gene="asnS"
                     /locus_tag="PK937_10500"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNH33045.1"
                     /translation="MRSRVYIEDVFKGKFSDGEEVTIKGWLYNKRSSGKLNFLLVRDG
                     TNTIQATVYKPSVTPESWEAADSASQESSLSVKGKVKKDERAPGGYELQVTEVVLLGY
                     AKDYPISPKEHGIDFLMDHRHLWLRSKQQHAVLRIRHTIIKAIRDFFDGRGFTLVDTP
                     IFTPAACEGTSTLFETDYFDTKAYLTQSGQLYAEAGAMAFGKVYCFGPTFRAEKSKTR
                     RHLTEFWMVEPEVAFNDLYDNMELAEDFLEYIVQTVLKDRMEELKTLERDITKLECVK
                     APFPRVHYDEAVKILKEANQPFEYGNDFGAPDEAIISAKYDRPVIVHHYPSAVKAFYM
                     KRDPNEPDKALAMDILAPEGYGEIVGGSQREDDYEALLSRIQEHKLPVEAFDWYLDLR
                     KYGTVPHAGFGLGVERTVTWICGIQHLRETIPFPRMIHRLKP"
     gene            12864..13865
                     /locus_tag="PK937_10505"
     CDS             12864..13865
                     /locus_tag="PK937_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33046.1"
                     /translation="MKKSIVLVLIGCLMVLWGCDDNNPYSPRLVLNLTDVYPDILYVS
                     EIFGSQHINAIHDTARGDILDISVNDSELDPTWSADGRKFAYTNIHLFNQSTGQPLHS
                     NIYIRDMDSSTLTPVTYSKYYLDTNFTFQGIVLNMRPDWSLMTNKMVFISNRDSTFDI
                     YTTTITDTLYGDTTAVRLTDETDKLDMFCHPSWSPDGSKIVYTSSKTGNEEIWIMNSD
                     GTGKTQLTDLNASIAGRPRFSPSGDKIAFYSSSSKNGNDSLNIFTMNPNGTGIQKVTQ
                     SGNNIDPAWTPDGNRIVYSKRTSSRGYIYLINRDGTEEKKMISKDSRAYYPIWRPAH"
     gene            13928..14476
                     /locus_tag="PK937_10510"
     CDS             13928..14476
                     /locus_tag="PK937_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33047.1"
                     /translation="MMTTKPSLKRQMVGILDQFLRGVISARELRSAALPLLQISAHKR
                     GRKNYIERYLVDISGKKDSELTRDYIYELRDMIIGDAVTSEKDRGRVFKRTLRKLIER
                     YFYEEIEEAYYLSLLYDLMFEFDSEVEREPAVKNFFAQIQSATPIHDDQMTVIEIAET
                     QDVIYDKTMDFYEEYFKSLWDD"
     gene            complement(14483..16873)
                     /locus_tag="PK937_10515"
     CDS             complement(14483..16873)
                     /locus_tag="PK937_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNH33048.1"
                     /translation="MTTHFDIQPLEFDKLKELIRQCVLTPMADACVQAMKPMTDADLI
                     EKRLDMLVEMADLLRYDDTFPIDTLTDIRQLLDRIHSHGTFLPSSDLLLVARFVETTR
                     KIKNYVRHRHEKYPLLSRDTDTLHDLRTIEDAIQHAIDDHGNIKDHASPKLRQLRRDI
                     DNKSHSLRKKLESLARQFTDAGYSQEAIVTMRDGRMVIPVKDEYKNTVRGFVHDTSAS
                     GQTVFIEPAEGLELNNEMRRLLIDEQREVERILIEIADTIRADRPALYENIDIATMIE
                     FLFAKARFCNTLQGIKPKLNTSGYIQIKKGFHPLLLHKENQKMPDQRRPVIPLDIEIG
                     KNFRTLIISGPNAGGKTVALKTVGLFVLMTQCGLLIPCGFDSELSLFTRLFADIGDDQ
                     SIENDLSTFSSHMQHLSEMASQLDDQTLILIDEMGSGTDPKEGASLAIALLEYFNNHR
                     AVSIVTTHHGELKAFAHNTPGVENGSMEFDQATLEPTYVFRPGIPGSSYAMEISRRIG
                     LHPDIIERAKSLSGTEALRLETLIHDLQSRVKKYETDLADINREKTGLEGMVKFYNDR
                     SKELKQKERALKQTILEEKQRVLAEANKKIEATILEIKKAEASKEAIKSAKTSIQNER
                     LATDQALADEKKLTEFVSEPLHVKKGDRVYIRHMDIEGTVIESPENGGQALIAVGAIR
                     LKIDPRQLSPAQNAGEKNSSPARQTIQWDTDDIGQELDLRGLTAEEALYEVDKYLTDA
                     GILGFREVTLLHGKGSGILRNRINEFLKRDRRIDSYRLGQYGEGDTGVTVVVLKND"
     gene            complement(16877..>17072)
                     /locus_tag="PK937_10520"
     CDS             complement(16877..>17072)
                     /locus_tag="PK937_10520"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33049.1"
                     /translation="LYIHDGQIKASLSGEQSSGVLSHMAYAQALLDIPENDGDKNKND
                     TVDAVVLDRDHLNQLIISIL"
BASE COUNT         4736 a         3690 c         3840 g         4806 t
ORIGIN      
        1 cattctctgc gcggcgtatg cattcaattc gctcaatacg cctgttctga cgagtattta
       61 tcggagcaac tatcccgatc attcgcgggg caaagtgatg ggtatcgtgc gcacagtgtt
      121 taatatcgcg tttgttgtgg cgtcgtatgt tttcggcctg atgctggatt tggacgggca
      181 taattatgtg tacgtgtacc ccattatcgg tttggtcgga ttgtggggtg ggtggcaatt
      241 gcgaaaaatc aaaatcgttc cgaccgtcca tactacgccg ccgatgcatc cgatccgatc
      301 cgccttgacg attttgcggc aggacaaacc gtttgctcat ttcatgggtt attgggcgat
      361 tttcggtttt gccatgctga tgattgatcc cgtaaaaact atttacgtga cggatccggc
      421 ctacgggatt aatgcgacgt acgaagaagc ggtgctcgcc gtgacggtga ttccgcaagc
      481 ggtgatgctt ttgacgtttg cattttgggg gcggcaaatt gataaatacg gtgtgatcaa
      541 aattcgtttt ctgctcaata ttttgccgac gatcaatgtg attttgttct attttgcgac
      601 cgatctcaga ctgatttatt ttgccagctt tttacaaggt atttccatga gcggtgcgca
      661 gctcagttgg caactctgtg tgatggagtt tgcgccgcgg cagcaggtgg gtttgtatat
      721 gggaattcac acgatgctga ccggtctgcg tggtattgtt gcgccgttta tcggtgcgtg
      781 gcttatcggt acatcgggta tgggaaatac atttttattt gcggcaggtc tgatggtcat
      841 cagtacggtg atgatgattc gtttttcggc taatcatatt cgacgtgata tcgaagtccc
      901 tctcccgatc gccgccgaac gcggagatta agtttctatt caaatcaaat aactcaaccc
      961 caaatcttca attctaaatt ctatatccgt ttcagatcat tgacggctct aaatttttat
     1021 tcctatttgg gctgaatatc tcactcgctt gcgacgtcgt agcttatcaa ctttttatga
     1081 ttgtcttgct actttacaac cttcatgtaa aaattcacgc gttttgttct attcaaggaa
     1141 ccaaaaaccc cgactgcttc tttgccttta atatttgttc tttcaggcaa gggagtcgtt
     1201 tcctgattgg aaatgtaagt aagcaaatca attggagcgg ttgaggtttc ttcaggacta
     1261 tacatacgac cgtaattcgt atctaaagcc attacttgaa tccaaccgct aaaaatgaaa
     1321 ggtaattgat aatttacagt atccggagta actgtgaaaa accctggagg ttcgtaaaca
     1381 tgattaatga agttaataaa aaattgactc gtcaaatgat cggttctata aaaaaatttt
     1441 ccttgactac tggtccaata tggagggtaa tttgctgaat aatagtagct gcgctttatt
     1501 gccacaacag tcagtgtatc gtcctcattt atgttagaaa tttgaaaatc ccctgggaca
     1561 gttgtttccg cagtaaaggc tttctcagtc gtagttactt ttaaactata tcgatgcaat
     1621 gggtgaacat gcaattgttc attcacatct ctatagatac catttccaat atcctgaaac
     1681 gtaacacttt cgttgtctgt tatatcaatc acttctacgc tcgcacctgt agtgccgact
     1741 atcttgcccc tgtaatcgtg ccatgtgacc agattctgga tatgaagcac ggggtcttct
     1801 ccatattgtg ccgtatcaac ctcattgctg tgaaccagaa tatctaacat ttttccaaca
     1861 aagatttctt gacgataggt tctgaaagaa tctggcacaa gaatcccaac gatataatta
     1921 taatctttat ccaaataagg agaaccttca ggcgcatcat tgcaggaaca aagtacggaa
     1981 aacatgatga tcacaataaa tgtatgcttg attctcacgg aagcctctca acagatgttt
     2041 acaagtttta atttactgct ttacagcctt catgtaaaaa tttacgcgtt ttgttctatt
     2101 caaggaacca aatacacccg caacgtttct cccttttatg tttgttcgtt tagataacga
     2161 tgtagtctct tgatcggaca agtaggtcag taaatctgtg ggcccagttg atgtctgttc
     2221 aggactgtac atttttccgt agtttgagtc taagcttgtt acttcaatcc acgaagcaac
     2281 cactgtatca atagatactt ctgttgtatc aattatgagc gggataacgc taggaggaga
     2341 ataaatgtgt ttcaaaataa tcggaggaaa taggcttgta gaacgtaaag ttcggtaaaa
     2401 aaaagttgaa ttactaagag accaataagg gaaaaaatcg ccaccgtacg tgtttcgata
     2461 aggacgaaca gttacgtcaa ccgtatctcc gtccgtaaca tttgataact ggaaattgcc
     2521 gggtacagtt gtggttgcct cgcttgtatc gttgccgatt gttacaataa gagtataagt
     2581 tatcattgat ttgattttta attcatttgc aacatcttga tagaacccat ttccgatgtc
     2641 cagtaattga acctcggtac catattgatc tctaataaaa actgttgccc cgctagtccc
     2701 gactaacttg ccccgataat catgccatgt tactaagttt tgaatataaa gtaatggatc
     2761 ttccccataa tgagttgtat ctacttcctc gcttccaatc ttgatatcga gcatttttcc
     2821 gacaaagact tcttgtcggt aagctctaaa tgaatcaggg acaagaatac cggtaatata
     2881 aacatagtca cgttctaagt acggcgaatc ctctctttcg gggtcgccgc acgaacaaga
     2941 aatagcaaga aaaaataaga atactacaac atgatatttc actacttcac catcttaaag
     3001 taaaaagaaa tcctcttggt gcgattgatc gagccaaaga ctcccgtggc atcgcggcct
     3061 ttaatgttgg ttcgtgacgg tagaggttta gtctcttgag ttgataaata agtcaataga
     3121 ggaatcggag ccgtcgaact ttcttccgga ctatacatcc ttccgtaatt actatccatg
     3181 gccatcacct gaaaccagcc gctgtaaagt aatggcagct tgtgattgag cgtatctgcg
     3241 ttcaccaaaa aaaaaccagg aggactataa acatgattga taacattgaa ttcgaaagcg
     3301 ctggcatggt gatctgtacg atagtaaaat gtgcctgaac tgatggtcca gcttggtttg
     3361 tgctccgccc aatagaaata ctcctcacgc tgagcagttt cgataattgt atctccctct
     3421 attagattgg tcacgtaaaa atcacccggg acagatgtct cagccgtaaa agttctacct
     3481 tttgttacaa ccgttagctc atagtgattc aacgctttaa tctttaatgc attttcaacg
     3541 tcacgataga aacctttacc tatatcctga aataccactt catgattgtc ctgatccttg
     3601 atctttacag tcgctccttc cgttcctaca agcttacccc tgtaatcgtg ccatgtaaca
     3661 aggttctgaa gccacaaagt agtgtcctcc ccatacatat tgatatcgac ttcgcctgag
     3721 cccacttttt catccaacat ccgtcctacg aatatttcct gccggtaagt acgaaaggag
     3781 tccggtacaa gtattccggt gatataatta taatccttct ccagataagg agaaccttca
     3841 ggcgcatcat tgcaggaaca agatacggca aaaaagagag ccactagcgc aatggtttgt
     3901 ttcatttaca tttcccttca aaaataagaa agacagggat atggattttg catatccctg
     3961 tcttgattaa tcacattaat acttccgtac acctacccaa tagtcgatat cattaagaat
     4021 ccggccttcc aacttaatat agtcgtttaa ataaccagac cccagtttcg cattaaccgt
     4081 ggctacatta atcttatcga tacccgactg accgtagata tcgtcaaagt cggtagaaaa
     4141 caatgttccc ccggccacat aaaatttcca taaatttgtc ttgaccgact gagcgaatac
     4201 atcgtaatgt ttgatatcta ccgaaatagt cgaaccatcg ttgacattgt cgtgtttacc
     4261 ggcgtcatcg tcattttcaa tatccacaac acgcatccac ccattaggat ctatccactg
     4321 caaaggccaa atagctatat cgtttgacat gatttcccat ccctgatcac cattgacatc
     4381 acggtaatag accgatcgtc ctgcggcatc gctgcgggtg tttccgttaa acttatgaat
     4441 cgtatgccct cggatttctc ctgcattttc ggtggattcc ccgacataca tttcaaattc
     4501 atcattggtg taatctttct tatcaaacag attgatctgc ttaaccacaa aatacggaac
     4561 cgtaaccgga accggtgggg gcgggggagg cggaggcacg ggacagtcct cgcaaggcgg
     4621 tggcggtagc aatgcgagct gagatgaatt cgacttaaca accctatccg catattcttt
     4681 aatcctcttt tgaagcaacg cacgaaactc cgcatcaatg atcggatcat cgccggtttg
     4741 ttcgagcgaa atcaaaaaca cgggatattc gatggcgtct tttgccgctt tcatcgcttc
     4801 tccgcgctct tccgtatcgg acgacatagg gagagaaatc gtcttctctt cgatagactt
     4861 tgaatcttca ttccaaaaat aaacagggat atcggattca tcagcatgaa ataagtccgg
     4921 atcaagcgca acgccataac tgaatttctt atccttctta tccaacgcaa aagcctgttt
     4981 tgaatttaaa agctcaactt tgattgtata atcaactccg tccagcgtta tctgggacgt
     5041 tgccgcttca acagccagtt ggtcaatagg cacgccggct tttttggtgt cttgtaaaac
     5101 tgactttttg agccacttct tatccattgc aaaaaaacgg gatagttctt ttatgccttg
     5161 attggctact cgttttgcaa ggcctcgggt ctcaaatagc tctttgcggg acgaaatatt
     5221 aatctgaggg aatgcggcgt tttcatcatt cgcatggaca ggctgttcat tgtctgaaca
     5281 cgaattgatg gttattgccg ccaaataaaa cccaaatagg gcggtagcaa ggaattttag
     5341 tttcattact taatcctctt tctgaggaac aaaagttgca aatccacatc gcatttcata
     5401 gatttgcgac gggaagacct tattactatt aaaattcaaa gctccaagcg ctgaattcgg
     5461 gtaatatggt tcttcctttt ttttgttctc atgttaatac cttgagttaa ttaaaagtct
     5521 gcgtaaacct gtttagacga ctaagactac acacctccct ccaaaacgtt agttgtacaa
     5581 tagctattaa agcgaagcta tagtcatttt aaaaatgaca ttatatgata cgtctttgtt
     5641 ctaatcccat tggaaataga aatagaattg gaattattgc taagctagag cttccctcat
     5701 ttcctgaagt tcgtgaaaac aactaccaat aagacagata ttcataaaaa aatgcataaa
     5761 cttctttttt aaatgaacct ttgtgtgagc gttacgctat aaaattcagc aagtcttgcc
     5821 gtaaagcatt gaaattatta ccataaattc gtgtatcgcg gcttgtcttt gtataaacaa
     5881 gccttaaatt aaccagtttc gagaggggta gttattagga gtaatatcaa atttagttca
     5941 gtaaaagagg caagtttaga tgcagttatg ctaaacattc cgtgaaatcc atctcttcgt
     6001 ttccctcatt ctctacgcaa ctcaatcaca aagtttcccg ccagactttt gaattcctga
     6061 acgtcctaat tctaaattct cttctccctc aaagcttcaa tcaataaatc cacatacgac
     6121 ggtttgctca tgcgcatcca gtgtttgatc aatacggtgc gcagactgag aaaaaagcga
     6181 tcatcggttt tgagtgcgcc ggtggcgtgc tttaccgacg gagcgtccac gacaaatttg
     6241 gataaaataa agggcgcttt gggattgagt acggaaaacg cttcatatat agaggtattc
     6301 agtttgtttt gtttttggag tgaagcggtt ttggaggccg cgatcggttt gtaaaaacta
     6361 tagatgtcca aatccggatc gtgcagcgga cgaaatcccg attccgattc aatccggcga
     6421 tgcaattcct gtgcggccag aaaattttcc gtcaaaagtc gtccgtagcc ttcttcgtgc
     6481 aaaggcatca tacggtgcgt gagccagcag cctgccgcga cggcgcccgg gcgcgaacct
     6541 tccagcgtaa acatgccgat attgggtttg tctttgatgt ggtaagtgta cggcgctgta
     6601 ttgagtatcg ccgatcgcaa ggcttcactt ttgtagatca ccgcgccgca tccgtatccc
     6661 aaagcaccct gtttgtgcgg atcgatcgtg atcgagtcgg catctttcat ggcccgcaga
     6721 ctgagataaa cttccggttt gacgatcagc gcgacatttg catagggctt gatacggccg
     6781 gatgcatcta taataatcga acgcgaatat ccgccgtagg ccgcgtcaag atgcaggtga
     6841 aacccgtaac gttttcgaag ttgcaaaata ccttcgatat catccaccgc gccgacacct
     6901 gtcgtgccga tattggcgac caccatcatg gttttctttt ttttcagtac agactcaagc
     6961 gcatcgaggt ccatgcgaaa tttggaatcg gtgggaattt ccacaccgtc ctgtatggca
     7021 aggatggagc aaatgcgttt ccatgaatag tgcgccatat gtgaaaaaag cacggcgcct
     7081 ggttttttat gtttttgtgt atatacgtcg cgtaccgccc acatggcttc ggtattggca
     7141 agcgaaccgc cgctgcacag atggccccaa ccttttttga aaccgcagag attgagcaaa
     7201 tcctgtacgc attccatttc aagttcggtc gtgatcggcc cgccttcgta ggcatggtta
     7261 ttgggattgg taagtatggc ggcaagataa cccagagtgg agctgagcgc cggatctttg
     7321 agcatctggg ccgcataacg cggcgaaaaa tacggcggct ggtgactgag acgttccaaa
     7381 agtacatcca gatgtttttg caaggtattg tctgaaggcg tcgccgagcg atcaaacacc
     7441 tccggatcgt tcgtaaaaaa cgtacgccgc cacgcggcat gccagtcgag aattttgatc
     7501 agcgattctt gcaaggcttc ggcattttcc gccttgggcc cgagaaaatt atcgtgcagt
     7561 atggacatga aaacccctca agcacgtgga aaaaagagaa ctgtattaaa acaaactaat
     7621 tcatatgaca accgcaaccc gcgccgcatc cgccgcccat catgggcata ctttcgccgg
     7681 agcttttgca gtcggcggca aaaacggaaa gcttgcggtt ggcctcatgg gcacggcatt
     7741 tgggacactc gacttccgaa gcgtcaaacg tgctggaatg aaaaagttca gaaaaatccg
     7801 taccgcattt tttgcaggta aattcataga tcggcatgta aagctccaca aatagattat
     7861 tgatttattg acgcgattta tttacgttca agccctaatt ttcaggcttt tatgaacata
     7921 aaataaaatg ttatctcaat caagaggatc gaatggagac caaaaatttt gatgtagtgg
     7981 tattgggcgg cggccccggc ggttatgtgg cagcgatcaa agcggcacaa ctcggtttca
     8041 aaacagcggt tgtcgaacgt gaaaaaatcg gcggcgtctg cgttaatatc ggttgtatcc
     8101 cgaccaaagc attgctgaaa aatgccgaga tgtatgaaca tatgaaacat gccgccgaat
     8161 ggggttattc ttttgacaac ctcaagtatg actacaaagc catcatcaaa cgcagtcgcg
     8221 atgtggccga tgccaattcc aaaggcggcg attttttgat gaaaaaaaat aaaatcgaag
     8281 tcatcaaagg aaccggcaaa tttcttgata aaagtacact cagtgtggcc gatgcgaccg
     8341 gcaaagaaac cactcaggtc aaaggtaaac atatcatcat tgccaccggt gcccgcgcgc
     8401 gtatgctgcc caatctgaaa gccgacggca aacgcattct gaccagcacg gaagcgatga
     8461 ccaataccga acagccgaag tccatcgtta tcatcggcgc cggcgcgatc ggtatcgagt
     8521 ttgcctattt cttcaatacc ttcggcacga aagtcactgt cgtggaaatg atgccgacga
     8581 ttttgcccgt tgaagatgaa gaagtctcca acacgctgct caaagtactc cagaaaaaag
     8641 gcatggatat tcacaccgat accaaagtcg aaaaagtgga tgtcggtgcc aataatgtta
     8701 ccgtgaccgt ttccagcaaa ggtaaaacac agacgatcac ggccgatcaa tgcctcgtcg
     8761 ccatcggcgt gcagggcaat acggaaaata tcggccttga aaaaatcggt gttaccgtcg
     8821 aaaaaggttg gatcaaagtg gacgagtatt accgtaccaa tgtacagggt gtttatgcga
     8881 tcggcgatat catcggcgca ccgtggctcg ctcacgtggc ttcccatgaa ggcatcgtct
     8941 gtgtcgaaaa aatggctgga aaggataccc acccgattga ttattcatcc attcccggat
     9001 gtacctattg ccaaccgcaa gtcggcagca tcggtctgac ggagaaaaag gcgcgtgaaa
     9061 aagggtatga tatcaaggtc ggtaaatttc ctttttccgc cagcggcaaa gcccgtgcga
     9121 tcggcgagcg tgacggtttc gtaaaagtga tttttgatgc gaagtacggc gaattgctcg
     9181 gcgcacatat cttaggttcc gaagcgacgg agatgatcgc cgaatacggc atcgcgaagt
     9241 ctttggagtc cacatataaa gaaataggca atacgattca cgcccatccg acgttgtccg
     9301 aagcgatgat ggaagcggcg ctggatgcgt acaaagaagc cgttcatatt tgattttttt
     9361 acaatgaagc tgagagcaac cggaatgcgt tgattccggt tgtttttttt agggcagatg
     9421 ggtatactac cgaagcccga caggaatgca tgatcattaa gtccgtcgaa ttcatagcca
     9481 gcgtctataa accggaacag gtacccaaac cgcagttgcc ggaaatagca tttttcgggc
     9541 gctctaatgt gggcaaatct accttgctca atacgttaac cggcaaaaaa atggcacgcg
     9601 tgagttccac gcccggtaag acgcagtgcc tgaattatta taaaataaat gatgcgtttt
     9661 atcttattga tgcgccgggt tatggctacg ccaaagtcga gcggaaactt agagaagcct
     9721 ggcaacgtaa tctaggcgcg tggctcacgg aaaacgatcg tatccgtctg gcggtactta
     9781 tcattgatgc gcgtatcgaa ccgcaggcgt cggatcttca gatggcggaa ttcctcaatt
     9841 attatcaagt cccgactttg atcgccgcca ataaagccga taaactgaaa aacagcgagc
     9901 gaaaaccgat actcaaattg ctcaatgaag cctacggcac atgggaatgg ccgattgatt
     9961 ttacgtccgg cgaaaccgga gaaggcaaag tggctgtttt gaaagccatg gattcggcat
    10021 taacgcatta aacgaaatta tattaacccc ggtctaagtc agaccggatc attgtgtaag
    10081 gagttggcat gaaacatcgt attctcttcg tgtgttgtgt gatgctgtgg gcgagcatga
    10141 cgcaaatcaa tgcgcaaaaa ggttccggca atcagagttc ggggcggttg tcgttttatg
    10201 tggattatgc ggcatttaaa atgttcaatc aaaaaaatgt tacctacacg cagttgtatt
    10261 tttatatgtc ccgcaatcag ttcaattttg tgaaaaaaga tacatcgtac tcggcggcat
    10321 atacggtgag tttggagttt accgatctca acgacagcaa aaatgttgtt caaaacacat
    10381 ggacaaccgt aatggatgat cgcattgcgg aggcggacac gggcaaagaa gtgcctttgt
    10441 tgtttgaata tcctttattg ctgaagccgt caaaatatat tctcaaagct acgatacagg
    10501 ataaaaacga tgcgtcccgg agcggaagct ataccgaatc cattgagata aatgattttg
    10561 ataaaagtga cttgatgatc agccaaattc agtttgcgac gcaagcgctc aaaggcggaa
    10621 atagcggtga aatgtttgta aaaaacggat atacggttta tcccaatccg acacgttttt
    10681 acggctccaa tctgcctcgg ttatcttttt attcggaagt gtacaatctg aaacatacgc
    10741 cggatgatgc gacgacaaat acgtatacgg cagaatttat cgtaacggac gaaaacggaa
    10801 gcatcatcaa agagtacccg tccagacaga tcaataaagc cggtgaaaca tccctgctta
    10861 tgcataacgt gaatgttatt tcgctgccga gcggtcgtta tgtttttatg gtgcgcatca
    10921 cggacaatgc cagtaaatcc gttgctataa gcaaaaaatt attcgtggtc tatcgcgaag
    10981 gcgaatccat ttatgagact caagcggacg attcgttttt taaagatttg gacgaagccg
    11041 gagccatccg tgcaggaaat atcgtttcgc tcatcggtcg cgaagatgaa aaaaagattt
    11101 tttcgcaatt ggaatttgaa ggtaaaaaga agtttttaga tcgtttttgg aaagatcgcg
    11161 acccttcgcc gggtactaaa tccaacgaaa aacttatgga ttattacaag cggtatgaag
    11221 aagctaatat gaagtttaca acgcccaatg tgcaggggtg gaaaacggat atgggtcggg
    11281 taatggttgt ctatggtttt cctgcgcaaa tcgaaaaaca tgaattcgaa tccgatatga
    11341 aaccctatca aatttggtat tttcttcaat taaaagatca accggcgcag actatttttg
    11401 tatttgctga tatggatgaa tccggtacat taaggttaat tcactcgaat gcgcgtggcg
    11461 aaatcgcttc acccaattgg caaacaatga taaaaaaata atttaattca ttttttaata
    11521 cctgatgaga gtatgtgtta tgcgttcgcg tgtttatatc gaagatgttt ttaaaggtaa
    11581 gttttcagat ggtgaagaag tcaccatcaa agggtggtta tacaacaaac gttccagcgg
    11641 caagttaaac tttttgcttg tacgtgacgg aaccaatacg atacaagcta ccgtgtacaa
    11701 accgagcgtt acccctgaat cgtgggaggc tgcggatagc gcttcacagg aatcaagctt
    11761 gtcagttaag ggaaaagtaa aaaaagatga gcgtgcaccc ggtggatacg aattgcaagt
    11821 gaccgaggtg gttttattgg gttatgccaa ggattacccg ataagtccca aagagcatgg
    11881 aattgatttt ctgatggatc atcggcattt gtggttgcgc tctaaacagc agcatgccgt
    11941 ccttcgcatt cgccatacga ttatcaaagc cattcgtgat ttttttgacg gtcgtggttt
    12001 tacactcgtg gatacgccga ttttcactcc ggcagcatgt gaaggtactt cgactttgtt
    12061 cgaaacggat tacttcgata ccaaagccta tttgacgcag agcggtcagc tttatgccga
    12121 ggccggcgct atggcattcg gaaaggtgta ttgcttcgga ccgacatttc gtgcagaaaa
    12181 gtccaaaaca cgccgacact taaccgaatt ttggatggtg gaaccggaag tcgcatttaa
    12241 tgatctgtat gacaacatgg aattggcgga ggatttccta gagtatatcg tacaaactgt
    12301 attaaaagat cgtatggagg aattaaaaac tctcgaacgc gatatcacca aattagaatg
    12361 tgtaaaggcg ccgtttcctc gggtacatta tgatgaggcg gtaaaaatac tgaaagaagc
    12421 gaaccaacct tttgaatacg gtaatgattt tggtgcgccg gacgaagcga tcatttcggc
    12481 aaaatatgat cgccccgtga tcgttcatca ctatccatcg gccgtaaaag cattttacat
    12541 gaagcgtgat cccaatgaac cggataaagc cttagctatg gatattctgg cgccggaagg
    12601 atatggcgaa atcgtcggtg gcagccaacg tgaagatgat tatgaagcgc tattgtcgcg
    12661 cattcaggaa cataagctgc cggtcgaggc ttttgattgg tatttggatc ttcgtaaata
    12721 tggaacggtt cctcatgccg gttttggact tggggttgag cgcacggtta catggatttg
    12781 tggcattcag catctgcgtg aaacgatacc tttcccgcgc atgattcatc gtttgaaacc
    12841 ataaaagatt aaaaggaata aatatgaaaa aatctatcgt tcttgtgttg atcggatgtt
    12901 taatggtttt gtggggatgc gacgataata acccatacag tccgaggtta gttctaaatt
    12961 tgacggacgt gtatcccgat atcttatacg tttcagaaat atttggttcg caacatatta
    13021 acgcaataca tgatacggca cgaggcgaca tattggatat tagtgttaat gattccgaat
    13081 tggatccaac gtggtcagcc gatggtcgca aatttgctta tactaatatt catttattta
    13141 atcaatctac gggtcaaccg ctacactcca atatatatat ccgtgatatg gatagttcga
    13201 cacttactcc tgtcacttat agcaaatatt accttgatac gaattttaca ttccaaggta
    13261 ttgtcttaaa tatgcgcccg gattggtctc tgatgaccaa taaaatggta tttatttcca
    13321 atcgagattc aacttttgac atttatacga ctacgattac agatacgctc tacggtgaca
    13381 cgacagctgt tcgtcttacg gatgaaacag ataaattaga tatgttctgc catccttcat
    13441 ggtcacccga cggatctaaa atagtttata catcgtctaa aacggggaac gaagaaattt
    13501 ggattatgaa ttccgatgga acgggaaaaa ctcagcttac cgatttgaat gcctctattg
    13561 ccggtagacc acggttttca ccatccggcg ataaaattgc attctacagc tcctcttcaa
    13621 aaaatggcaa cgacagtctg aatattttca caatgaatcc gaatggtacg ggtatacaaa
    13681 aagttaccca atcagggaac aatattgatc cggcatggac tccggacggt aaccggattg
    13741 tatattcaaa acgtacatcc agtcgcggtt atatttatct tattaaccgc gatggcacgg
    13801 aggagaaaaa aatgatatcc aaagacagtc gggcctatta tccgatttgg aggccggctc
    13861 attaacccat atttaagtgt tttcggaaaa accttcagtc aatcctgaag gttttttcgt
    13921 attaactatg atgacaacaa agccatccct caaacgtcag atggtcggta ttcttgacca
    13981 gtttcttcgc ggtgtcattt cggcgcgtga actgcgttcg gcggccttac cacttttaca
    14041 aatatcggcg cacaaacgcg gtcgaaaaaa ttatatcgaa cggtatctcg tggatatttc
    14101 cggtaaaaaa gattcggagt tgacgcggga ttacatatat gaattgcgcg atatgatcat
    14161 cggcgatgcc gtcacctcag aaaaagatcg cggaagagtt tttaaacgta cattaagaaa
    14221 actgatcgaa cgctattttt atgaagaaat cgaggaggcc tattatttgt cgcttctata
    14281 cgatttgatg ttcgagtttg attcggaagt tgaacgcgaa ccggcggtga aaaacttttt
    14341 cgctcaaatt caatccgcta cgccgataca tgacgaccaa atgaccgtaa ttgaaatagc
    14401 cgaaacgcaa gatgtgatct atgataagac gatggatttt tatgaagaat atttcaaaag
    14461 cttatgggat gactaaagcg gatcaatcgt ttttcaaaac aaccacggtg acaccggtat
    14521 ccccttcgcc atactgccct agccgatagg aatcaattcg gcgatctcgt tttaaaaatt
    14581 catttatacg atttctaagt atgccggagc ctttgccgtg gagtaatgtc acttctctga
    14641 accctagtat ccccgcatcg gtaagatatt tatctacctc atatagcgct tcttcggcgg
    14701 tcaaacctcg taaatcaagt tcttgcccga tatcgtcggt atcccattgg atggtctgac
    14761 gtgcgggaga agagtttttc tcaccggcgt tttgagccgg ggaaagttgg cgaggatcaa
    14821 ttttaaggcg gatggctcca acggcgataa gcgcctgacc accgttttcg ggagattcga
    14881 tgaccgttcc ttcgatatcc atatgtcgta tataaacacg atcgcctttt ttgacatgaa
    14941 gcggttcgga tacgaattcg gttaattttt tttcatcagc taaggcttga tccgtggcga
    15001 gccgttcgtt ttgtatggag gttttggccg atttgatcgc ttctttggaa gcctcggctt
    15061 ttttgatttc caaaatcgtc gcttcgattt ttttattagc ttcggccagt acgcgttgct
    15121 tctcttctaa aatggtctgt ttaagtgcgc gctccttctg ctttagttct ttggaccgat
    15181 cattgtaaaa cttaaccatg ccttcaagcc cggttttctc gcgattgata tcggccaggt
    15241 cggtttcgta tttttttacg cgggattgaa gatcatgtat cagtgtttcg agccgtaatg
    15301 cctcggtacc ggataaactt ttagcgcgct cgatgatatc gggatgtaaa ccgatacgcc
    15361 tggaaatttc catcgcataa ctgctgccgg gaatacccgg tctgaaaacg taggtcggtt
    15421 ctaacgtagc ttgatcaaat tccatggagc cgttttcaac acccggcgta ttatgagcaa
    15481 acgctttcaa ttcgccgtga tgcgtcgtaa cgatagagac ggcgcggtga ttgttaaaat
    15541 attcaagcag cgctatagcc agcgaagcgc cttctttggg gtcggtaccg cttcccattt
    15601 cgtcaataag aatcagcgtt tgatcatcca gttgagacgc catttcggac aggtgctgca
    15661 tatgcgagga gaaagtggag aggtcatttt cgatggactg atcgtcgccg atatcagcaa
    15721 aaagtctggt aaaaagactc agttcgctgt caaatccgca cggaatcaac aaaccgcatt
    15781 gtgtcatcaa tacgaaaagt ccgacggttt tgagggcaac ggtttttcca ccggcattgg
    15841 ggccggaaat aatgagtgta cgaaaatttt ttccgatctc tatatccagc ggtatcacgg
    15901 gacgcctttg atccggcatt ttctggtttt ctttatgcag caggagcgga tgaaatcctt
    15961 ttttaatttg aatgtatccg ctggtattga gtttgggttt gataccttga agcgtattgc
    16021 aaaaccgtgc tttggcgaac aaaaattcga tcatggttgc aatgtcgatg ttttcataca
    16081 gtgcgggacg gtctgcgcga atcgtatcgg cgatttcgat caaaatgcgt tcgacttctc
    16141 tttgttcgtc gatgagcaaa cggcgcattt cattgttgag ttcgagcccc tcggccggtt
    16201 cgatgaaaac tgtttgaccg ctggccgatg tgtcgtggac aaaaccgcga acggtattct
    16261 tatattcatc cttaaccggt atgaccatgc gtccgtcgcg catagttacg atagcctcct
    16321 gtgaataacc ggcatcggta aattgccggg caagagattc cagcttcttt cgaagactgt
    16381 gcgatttatt gtcaatatcg cgacgcaatt ggcgtaactt cgggctggcg tgatctttga
    16441 tgttgccgtg atcgtcgatt gcatgttgaa tagcatcttc aatggttcgc agatcgtgca
    16501 acgtatccgt gtcacgcgaa agcaaagggt atttttcatg acgatgacgt acgtagtttt
    16561 ttattttgcg tgtcgtttcg acaaatcgcg ctacaagcaa taaatcagat gaaggtagaa
    16621 aagttccgtg cgaatgaatg cgatctaaaa gctgacgtat atcggtcaag gtatcaatcg
    16681 gaaatgtatc atcataacgc aataggtcgg ccatttctac caacatatcc aaacgttttt
    16741 cgatcagatc cgcatccgtc atgggtttca tcgcctgcac acaggcgtcc gccataggtg
    16801 tgagcacgca ttggcgaatc aactctttga gtttatcaaa ctccagcggc tgtatatcaa
    16861 aatgtgtcgt catcgctcaa agaatggaaa tgattaattg attgagatga tcgcgatcca
    16921 atacgacggc atctacggtg tcatttttgt ttttatctcc gtcgttttca ggtatatcta
    16981 agagcgcttg tgcataagcc atatgcgaga gaacaccgga actttgttcg ccggataaag
    17041 aagccttgat ctgaccatca tggatataaa gg
//
LOCUS       DAOQGF010000073        16829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1574_length_16829_cov_6.414570, whole genome shotgun sequence.
ACCESSION   DAOQGF010000073 DAOQGF010000000
VERSION     DAOQGF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16829
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1574_length_16829_cov_6.414570"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="PK937_10525"
     CDS             complement(<1..1366)
                     /locus_tag="PK937_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNH33050.1"
                     /translation="MRRLSTILFVLILGYYAVSSYVSFYSELLWFQSMGYEEAFWRMY
                     MTEYIVGAVYFAIFWLVIGVNIWIASRMEAVYVSTTGGVYQQPFNLVAKPAKTIFFGI
                     LIFVSYIMSSAPAAKWMRILQYMHSESFGTADSVFQKDIGYYIYQLPFYQSIVSWSYG
                     VVIVAMIAVLATYLYRKAIYIEPQGIRLADVVKRHLMILGAMLLLIYAVDNYFSAYNI
                     LYKDNGTVVGASYTDVHAVLLGYRVMIAVAILGAIAVLWGAVQATWRFAVIGGVAQFA
                     VGFVMLNVYPGLIHRFVVTPNALEKEKPYIEENIRQTRRAYELDKITEQDFNYELSLK
                     ASDLKANSSTIKNITLWDYRPIKDSYSQLQEIRPYYNFYDVDIDRYMINGEYRQVMLA
                     GREMNLDKIGSSENWINKTFIYTHGHGIVMSPVNVVTQEGQPEFFIKNIPPELAVNIK
                     IDRPE"
     gene            complement(1408..2799)
                     /locus_tag="PK937_10530"
     CDS             complement(1408..2799)
                     /locus_tag="PK937_10530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNH33051.1"
                     /translation="MRKPFLCADGGSKNVYKRSSVYIFLSLILLLNGCVPQYGELKLP
                     PRHIIIKYCWETFELGLKDKNDFVKTSTLTTLGRIGSASAAETIASADLGVKPQVTRT
                     AVATLAQMHDSAAFQALLRFRGHSDFFVRENVAMGMVRMRDLYGDTLVARVLKRMYLY
                     VDSIESDTMFYEKSEIQQERREIKTKLAIAISSVDPRFPDPQIALAYRNAPLAARVTL
                     VNFIGAVKPPNALTWLSPFMIDSSTYVRSKTAEALGKIASPQAYDMLRGMLRTQRREE
                     VIVNAAIALMPADEARAVEVLIQAMDTYDEDTQSNILLALGEAKLPESRAKILPLVKS
                     AVTGKGDWVRIGAIGALGLIKDTTTLPLIEAALEDPVDEVREIAVGVLAQMKGETMVD
                     RLKAWAKDDQYSMRSVALAGLGSLRDPVIVEKTVYPVLYNTMKFDPEMMVRVRAAFTL
                     LNIINDRKYTKGI"
     gene            2898..3641
                     /locus_tag="PK937_10535"
     CDS             2898..3641
                     /locus_tag="PK937_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33052.1"
                     /translation="MNSEGTKGSPFPRSNRILIVEDDPDTIKILRLYFIKEKFEVDVA
                     GDGEEGLSILRQHHHDIVLSDVMMPKMDGYKLCEAIRSDDTIKMTPIVLVTAKVELSD
                     KLTGLEKGADAYLTKPYNLTELRAQIASLLKLRELRLALTNREKEIERIRTLEQTLIA
                     ISHHINNAIAPISGRAQLTDPADVEQVRKLIDVASIGCRRISRTINLLSDVVSAMKQA
                     SNDQAFDLNKTTINELLEKFKSNGDTDLQ"
     gene            3738..5171
                     /gene="dnaB"
                     /locus_tag="PK937_10540"
     CDS             3738..5171
                     /gene="dnaB"
                     /locus_tag="PK937_10540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNH33053.1"
                     /translation="MSVQEDQINGKILDYPGRVPPQATEVEEAILGAILIDEAAFTSI
                     MDFLDESCFYKESHRIIYGVMKTLYLRSEPIDALTVTEELRRSNQLERIGGAYFITGL
                     TNKVPSASNIATYTKIVLDKSRLRKLITVGGQIVNMAFQETEDVDTLIDTVEKQIFDI
                     AQDKASQQFRPLNAILHDAFEQMDNYYNRPGGLTGVPSGFQSIDHITNGFQASNLIIV
                     AARPGMGKTALVLNVARHAAVLHQKAVGIFSLEMSSTDLVTRLLCAEAEVDAQRLRSG
                     NLPDKDYRKLHTSVNNLHKANIFIDETAGLNILDLRARARRLKAEHNVELLIIDYLQL
                     INASVRSGANRQEEVALISRSLKALAKELSIPVIALSQLSRAVEQRGGDKRPQLSDLR
                     DSGSIEQDADVVMFIHREDYYNTAQDAFNQSMVTEAEIIIAKHRNGPTDTAKLGWARK
                     FTKFVPMDTAHTGIVPPPSSHDVEPAF"
     gene            5175..6215
                     /locus_tag="PK937_10545"
     CDS             5175..6215
                     /locus_tag="PK937_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH33054.1"
                     /translation="MLPASPLVSVVILNYNGRRFTDTLFESLRRTTYPHVEWVMVDNA
                     SSDDSVSYTREKFPWVRIIETGANLGYTGGNNVGILQSKGAYVVLLNNDVEADPDWLT
                     HLVNAAEADASIAALQPKLQSMINKGFFEYAGASGGFIDPWGYPFLRGRIFDTIEKDH
                     GQYDDVRDIFWASGAALFLRRSALDEIGLLDEDFFMHFEEIDLCWRLHWAGYRIQVIP
                     QSRIYHYVSASLPAANLRKLYWNHRNSLVVLIKNLPRRSFWRTLLFRFVLDGIAAIQS
                     LFRGEPLRMWAVLKAHFWIYAHCISLSSKRKFAKNLHKTDETSFAHLIYPRSIVADYF
                     LKKKREFHTMGF"
     gene            6236..7096
                     /gene="rfbD"
                     /locus_tag="PK937_10550"
     CDS             6236..7096
                     /gene="rfbD"
                     /locus_tag="PK937_10550"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNH33055.1"
                     /translation="MTILLWGGSGQLGKDIQRVLADQHHVIAISRSECDISDSAQVLS
                     ATEKHRPDAVVNCAAMTDVPGCEQRTEEAFTVNALAARHIADASRILNAYLIHISTDY
                     VFDGNKKTPYIESDLTAPVNTYGITKLAGDHFVNAYHPAAAVVRTSGLYGIFECRGKK
                     TNFVETMLRLGREREIVKVVDDEVLTPTYTHDLAGQIALMLNDKPAGIFHATNAGACS
                     WYDFTQTIFSIAGYTTRLERTTVAAFGSPVRRPAYSVLENAALQKLGIDRMRPWQDAL
                     RAYMTERSDK"
     gene            7129..10005
                     /locus_tag="PK937_10555"
     CDS             7129..10005
                     /locus_tag="PK937_10555"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNH33056.1"
                     /translation="MNRFAFINRITAAIVFAVAFLGYLFTISPTLSYWDCGEFAATAY
                     TMAIPHPPGSPLFLLLGRIFSILPTSGIGHALGLAVTDYDIAFRINLISTFTSAFAVL
                     FLYLTTVRLILQWKERPESTFGSLKIVIASAISALTFAFTYSQWFNAVESEVYAASIF
                     CTAIVTWLITVWLEKPDDIHSDVYLLLIAYILGLAIGIHLLVILALPFIFFLIYSKKF
                     EITFSGFFKFLLIGLIAMGVIYKVFLFYSIQIPFMLDRFGLSTVSVFLIFAVLIYLSF
                     IMIRNNNHNGALIVISSLLIFLGYSTYATIMIRSGLNPNVDQNDPDTWSKFISYLNRE
                     QYGELVLWPRNAPIWEYQIKKMFVRYFNWQFIGRPDEYALSLIDHLRNAIGWSVDKLQ
                     DTQEDRYSYMYTVLSFRGLYALPFLIGIFGMIHHFSKDWKRALSTLGLFIMTGIMIIV
                     YVNQPDPQPRERDYSYVGAFFAFSIWIGIGIYAIFETIEEKFKDSDMTRMLTYGVTGV
                     AFVLLPLNMFVYNKFSSSRQGNYVAWDYSYNLLVTCEPNAILYTNGDNDTFPLWYLQE
                     VEKVRPDVRIVNLSLVNTEWYINQMKNLEPTYHMPDGTTFKALKVPVGFSDREILGDP
                     KIPNSSIQPMRWDRRDFALDVPKDVYWKDWVESGRKLPPGYDTMTVPKMKFTVEPTIQ
                     GRGLRVQDLMVLDILFANKFERPIYFAITVSNDNMCGLDKYLRMDGLAMKLVGVPGTD
                     MSIDHMYENTFKKYKYRNMNNPNVAYDDNVRRLTQNYRTLYLRMAEYYRTQKNTSSGL
                     HTQIDDAYPAEFTTNQKIVAILDSMEKTIPQNVIPVRDYRLKLAIGQFYSDAGKPEKL
                     REAAEDILANEKAVRLDPSGKLRVAAMFLFNLKDAERAKKILEPMSQADPTNPEILGY
                     YTMALEQLGEYAQAAQVLEQWVAFNPKDVQAQAKLNELKSKIKP"
     gene            10010..10990
                     /locus_tag="PK937_10560"
     CDS             10010..10990
                     /locus_tag="PK937_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNH33057.1"
                     /translation="MPVTWQKYVWTVLKISVSLGLMYWAVRSIDLTVMIAIWQSINVN
                     WILASFILLTLSYLLGAWQWQSILAIGDIRLKYPTVVGYYYVGLFFNNFLISGMGGDV
                     LRVYDIHRHTSDPQRLSPALAVVFFDRFVGLLILILYACISGIFLIGQGESLRMFFAI
                     TGLLLLWIFALILLFHRPLAIFLLTPLAWLVPKRLRMRVDHLYSELNRFRTAPQLLMR
                     VFLIAGGVQMLRIIAIAAIGRALGDASSLMFYILFVPMISLAASLPISIGGTGPREQT
                     TVFLFRKIGVSSEIAFSIGFVTYLLSAVSTLPGGFIFMLRKNKAEHAVVS"
     gene            10974..11969
                     /locus_tag="PK937_10565"
     CDS             10974..11969
                     /locus_tag="PK937_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33058.1"
                     /translation="MPSYHKLLFVVLFLGSCKPTQSEAIGGTDTLILFCDSTLSPIIT
                     ASLHKTWFMPRAETAFDVQHQTMDQFTVYRHFRYLLFAGSLDAHDAVSKWIRSTLSPD
                     DEKAVREGQLWILEKESPWAQGQMIWVLCTPDYETMVRRCATEGIMLHRRLEEKRREQ
                     ETVMMQHDYFDAMPVQSADADTFYLYDGFSQQPAPMSKTFFWYRSTERFQEWILFSTF
                     TGDSVNISTSLGRHTQKLLNALPDSLKYRDDFITSDTLYTTSGPLLRMCTVWTTKNRT
                     NTFGGPLWLYVSLTPTNKPKKIGVAMAFGPQNERKHPAMRRLENLIIYNLTRSYE"
     gene            11962..12531
                     /locus_tag="PK937_10570"
     CDS             11962..12531
                     /locus_tag="PK937_10570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH33059.1"
                     /translation="MNNRIFTISNILSMLRIVFAVPILYFLHQGIEDPTANIYALITI
                     LIAGFTDLFDGYLARRLDQVTELGKIIDPLADKIVSAIILVYLALIRPDFPLWLLIVA
                     IVRDVVIFAAAMYVKQKYNYLFTSNMLGKWAFTVLGLTFAIFVVRNNFSIEWLFQSFL
                     WLTFIMLILSFYVYAQRLWVFMQNQRKNA"
     gene            12528..13532
                     /locus_tag="PK937_10575"
     CDS             12528..13532
                     /locus_tag="PK937_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH33060.1"
                     /translation="MKYFIITLFCLLTFGELSYAQVRGGNRNRIDKKNTVADDPQVAF
                     RMAFKAYKEKDIPKAQKLLKNTLDKHPTHVLSLILISKILVEQDDLKSAKKFIKTIPE
                     SEHHRPAIARILAEIAYRNGKPLKALQIINRTQSDAASSDDAEFFDNPNQDIASFKKQ
                     LLASTRQSQRKEMPYAKEKPATETHLTIPGDLPTVATERRLRLAIFSLSVAPSLDDTT
                     LGSSTASYLTTAAVQTQCFRVVERENLEKVMIEQDFQMTDAVDTETAVETGYIMGVDA
                     ILVGSVRPMAQGYEVDGRLIRVETGEIIAATNQSISSVSLVRQSAANLMFDLTRAAYK
                     "
     gene            13627..14061
                     /locus_tag="PK937_10580"
     CDS             13627..14061
                     /locus_tag="PK937_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33061.1"
                     /translation="MIVEKIRELAEDLRRTHQDLRVMQSDMRMQDGAYAREADIDRVI
                     SDLTTFKLNVTYLRLKAGELVQRNNLEFSEVREEVRYFATYLKHASDTFDEICNSMAY
                     GGRSGDDAAMQKTGEELLILQRHLQEMNEHLLVINSKLGKTS"
     gene            14082..14477
                     /locus_tag="PK937_10585"
     CDS             14082..14477
                     /locus_tag="PK937_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH33062.1"
                     /translation="MKQSKKVQIASESAVKVTTHRITDDLASVEIDDKMMTEEHMKML
                     EKAVHELLAENYHSIILDLTKLKRINSTGLGKFINLYKAAADKDGVMKIGGVSDFVRN
                     VLTSTKLNEIFEIHNTLDDAIKSFQTSGA"
     gene            14637..14712
                     /locus_tag="PK937_10590"
     tRNA            14637..14712
                     /locus_tag="PK937_10590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14670..14672,aa:Ile,seq:cat)
     gene            14869..15444
                     /locus_tag="PK937_10595"
     CDS             14869..15444
                     /locus_tag="PK937_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33063.1"
                     /translation="MRFSQIVSLFLIVSFVSFTACDSDSKSTVDASEQKPVLSLTQHD
                     STILTQAYVNSSWYEFGPRLRFLKNGTITGLGMMMPDSGSYEVTLWDLSDTSIVTTVT
                     IAADSGELKYVAIDPIQVQTDERYSVTILSDDWYWFEDISGKNNFLPQTFDDVMIEDY
                     AYISGSTHQFPTDAFTDYLAGIQDIIFVPEK"
     gene            complement(15599..16240)
                     /locus_tag="PK937_10600"
     CDS             complement(15599..16240)
                     /locus_tag="PK937_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNH33064.1"
                     /translation="MQYQSRNNRYTLMMDISVIITIVLAYIILHTVTITEYAKAEIRT
                     PTQIELTRVDLTASHHAPPPRRPVVPVATDQETLLGDETIDDTDIDLDETPPPPPPPF
                     KKVEKGDDDVFTVYDTRPLLKGGADFLKRNLKYPEAAQQAGIEGVALVRAVVDEKGNV
                     VKAEVVKEDGNVGFGQAAIDFVLKCKFEPATFKNKPVKVAVTLPITFSKKNLL"
     gene            complement(16244..16666)
                     /locus_tag="PK937_10605"
     CDS             complement(16244..16666)
                     /locus_tag="PK937_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH33065.1"
                     /translation="MKFRRRPIESLPVSSASMSDIVFSLLLFFMVSTVIKKYSGLPVQ
                     VPAAFNIEKLSTKTHTSYLWIDKQQRIVFDDYPINSMEELYSVARAKIEADHKMLIFL
                     RVDESLTMGALADVQEQLRRAGALRIYYGTDSRSNAIY"
BASE COUNT         4419 a         4015 c         3767 g         4628 t
ORIGIN      
        1 tttccgggcg atctattttg atattcaccg ctaattcagg cggaatattt ttgatgaaaa
       61 actcaggctg accttcttgt gtaacgacat tgaccgggct catgacgata ccgtggccgt
      121 gggtgtagat aaaggtctta ttgatccaat tttcactgga accgattttg tcgagattca
      181 tttcacgtcc ggccaacata acctgacggt attcgccgtt gatcatgtag cgatcaatgt
      241 ccacgtcata aaaattgtaa tacggacgta tctcctgaag ctgcgaatac gaatctttga
      301 ttggacgata atcccacaac gtgatatttt tgatcgtgga cgagttggct ttcaagtcgg
      361 acgctttgag cgataattcg tagttaaaat cctgttcggt gattttatcc aattcgtacg
      421 cgcgacgcgt ttggcgaata ttttcttcga tataaggttt ttctttttcg agcgcgttgg
      481 gagtgacaac aaaacgatga atcagtccgg ggtacacgtt gagcatgaca aagccgacgg
      541 caaattgggc aacgccgccg atcaccgcaa accgccacgt cgcttggacc gcgccccata
      601 atacagcgat cgcgcccagt atggcgacgg caatcatcac acgataacca agcagaacgg
      661 catgtacatc cgtatacgat gcgccgacta ccgttccgtt atctttgtac aggatattgt
      721 acgccgaaaa ataattatcc acggcataga tcaaaagcaa catggcgccc agtatcatca
      781 gatggcgttt cacgacatcc gcaagacgaa tcccctgcgg ttcgatataa atcgctttac
      841 ggtaaagata cgtggccagt acagcgatca tcgcgacgat caccacgcca tacgaccatg
      901 aaacgatact ctgataaaat ggcagttggt aaatataata accgatgtct ttttgaaaca
      961 cactgtccgc cgtaccgaag ctttcactat gcatgtattg caaaatgcgc atccacttgg
     1021 cagcaggcgc actggacatg atatagctta caaagatcaa tatgccgaaa aatattgttt
     1081 tggccggttt cgcaacaaga ttgaaaggct gctgatacac accgccggtc gttgaaacgt
     1141 acaccgcctc cattcgggaa gcgatccata tattgacacc gatcacaagc caaaatatcg
     1201 cgaagtatac cgcaccgaca atatattcgg tcatgtacat tcgccaaaag gcctcttcat
     1261 atcccatgga ctgaaaccac aacagctcgc tgtaaaaact gacgtaactg gatacggcat
     1321 agtaaccgag tatcaatacg aaaaggatgg tggacaaacg acgcatatgc gttgattagc
     1381 tccttgttag gttagcaata cttagcttca aattcctttg gtgtacttac gatcgttgat
     1441 gatattaagc agcgtaaacg cggcccgcac tcggaccatc atttccggat caaacttcat
     1501 ggtattatac aaaaccggat acacggtttt ttcgacaatg acgggatcgc gcagggaacc
     1561 taatccggcg agggcgacgg aacgcatgga gtactgatcg tcttttgccc aggctttgag
     1621 ccgatcaacc atcgtttcgc ctttcatctg agccaacact ccgacggcaa tttcgcggac
     1681 ttcgtccacc ggatcttcca gcgccgcttc gatgagcggc agcgtggtcg tatctttgat
     1741 caatcccaga gcgccgatgg cgccgatgcg cacccaatcg cctttacctg tgacggccga
     1801 ttttaccaat ggtaaaattt tagcacggga ttcgggcaat ttggcctcac cgagagcgag
     1861 caagatatta ctttgcgtat cttcgtcata ggtatccatc gcctgtatca aaacttcgac
     1921 ggcgcgcgct tcgtcggccg gcatcaaggc gatcgccgca ttgactatca cttcttcacg
     1981 ccgttgcgtt cgcaacatgc cccgcagcat atcataggct tgcggtgaag cgatttttcc
     2041 taaagcctcg gccgttttgg aacgcacgta ggtggaagaa tctatcataa acgggctcaa
     2101 ccatgtcagc gcattgggtg gcttgacggc gccgataaaa ttgaccagtg tgacacgcgc
     2161 cgccagcggt gcgttgcggt aagccagcgc aatctgagga tcagggaatc gcggatcgac
     2221 cgatgaaata gctatggcca gtttggtttt gatctcacgc cgctcttgct gaatctcgga
     2281 tttttcgtag aacatcgtgt cgctttcgat cgaatccacg tatagataca tacgcttgag
     2341 tacgcgggcc actaaggtgt caccgtacaa atcccgcatg cgtaccatac ccatcgcgac
     2401 attttcgcga acaaaaaaat cagaatggcc acggaaccga agcaaagctt gaaatgccgc
     2461 actgtcgtgc atctgcgcca acgttgcgac ggctgtgcgt gtcacttgcg gtttcacacc
     2521 gagatccgcc gatgcgatgg tttccgccgc cgaggctgaa cctatacggc ccaaggtcgt
     2581 cagcgtggat gttttgacaa aatcattttt gtctttaaga ccaagctcaa atgtttccca
     2641 acaatatttt ataataatat ggcgcggcgg caacttgagt tcaccgtact gcggtacgca
     2701 tccgtttaac aacaaaatga ggctcaaaaa aatatatacg gacgatcgtt tgtacacgtt
     2761 tttactccct ccgtctgcgc ataaaaaagg cttgcgcatg ggtaattttc tgtagtatgt
     2821 tgtcctgccc catagggcgc acaattgaaa atcaaagtat aaacaatttc ctcaaaagtc
     2881 aattttatcg ggcgtgcatg aactctgaag gtactaaggg atcgcctttt ccgcgcagta
     2941 accggatatt gatcgtcgaa gacgaccccg acacgataaa aatccttcgt ttgtatttca
     3001 tcaaagaaaa atttgaagtt gatgtagcgg gcgatggcga agaaggtttg tcaattttac
     3061 ggcaacatca ccatgatatc gtactgagcg acgtcatgat gcccaaaatg gacggctaca
     3121 aattgtgcga agccattcgc agcgatgata cgatcaaaat gacaccgatc gtgcttgtta
     3181 ccgccaaagt cgaattgagc gataaactca ccggcctgga aaaaggcgcg gatgcgtatc
     3241 tgaccaaacc atacaacctt accgaactcc gcgctcaaat cgcgtcgctg ctcaaacttc
     3301 gcgaacttcg tctggctttg accaaccgtg aaaaagaaat cgaacgcatt cgcaccttgg
     3361 aacaaacatt gatcgcgatt tcccatcata tcaataatgc catcgcgccg atttcaggac
     3421 gtgcacagct caccgatccc gccgacgtcg aacaagtgcg caaactgatc gatgtggcct
     3481 ccataggatg ccgccgcatt tcccgtacga tcaatcttct gagcgacgtg gtctctgcga
     3541 tgaagcaagc ctccaacgat caagcgtttg acctcaataa aacgacgatc aacgagctgc
     3601 tcgaaaaatt caaatcaaac ggcgatacgg acttgcaata gtccattttt tgattaactt
     3661 cactccccgc aaaactaaga tttagactga gttttgctgt tattatttga gtcacctaac
     3721 cattttttat aaaaaacatg tccgtacagg aagaccaaat caacggtaag attctcgact
     3781 atcccgggcg cgtaccaccg caggccacgg aagtcgaaga agctattctc ggcgccattt
     3841 tgattgatga agccgcattt acctcgatca tggatttttt ggatgaatcc tgtttctaca
     3901 aagaatccca ccgcatcata tacggcgtga tgaaaacatt gtatctgcgg agcgagccga
     3961 tcgatgcgct taccgtaacc gaggaacttc gacgctccaa ccaactggaa cgcatcggcg
     4021 gagcatactt tattacaggt ttgaccaata aggtgcccag cgcttccaat attgctacct
     4081 acaccaaaat tgttcttgat aaatcccgtt tacgtaaact gattactgtc ggcggacaaa
     4141 tcgtcaatat ggcgtttcag gaaaccgaag acgtggatac actgattgac accgtcgaaa
     4201 aacaaatttt tgatattgct caggataagg ccagccaaca gtttcgtccg ctcaatgcga
     4261 tcttgcacga tgcttttgag caaatggata attattacaa tcgcccgggg gggctgaccg
     4321 gcgttccgtc aggttttcaa agtattgacc atatcaccaa cggttttcaa gcttccaatc
     4381 tgatcatcgt tgccgcgcgc cccggtatgg gaaaaacggc cctggtactc aatgtcgccc
     4441 ggcatgcagc ggttttgcat caaaaagccg taggtatttt ttcgctcgaa atgtcttcga
     4501 ccgatcttgt gacgcgtttg ctttgcgcgg aagcggaagt ggacgcccaa cgcttgcgca
     4561 gcggcaattt gccggataag gactatcgca aactgcatac gagtgtcaat aatctgcata
     4621 aggcaaatat ctttattgac gaaacggccg gtctcaacat tcttgatttg cgtgcgcggg
     4681 cacgacgtct caaagcagaa cataatgtcg aacttcttat tatcgattat ctccagctga
     4741 tcaatgcgtc ggtgcgttcg ggtgccaatc gtcaggaaga agtggccttg atctcccgtt
     4801 cgctgaaagc gttggctaaa gaattatcca tacccgtgat tgctctttca cagctttcgc
     4861 gcgccgtcga gcagcgcgga ggggacaagc gcccgcaact ttccgatttg cgcgattcgg
     4921 gttctatcga acaggatgcg gacgtcgtta tgttcatcca tcgcgaagat tattacaata
     4981 cggcacaaga tgccttcaat cagtccatgg ttacggaagc cgagattatc atcgccaaac
     5041 accgcaacgg gccgaccgac acggccaaac tcggatgggc gcgcaagttt accaaatttg
     5101 tcccgatgga cacggcgcac acaggcatcg taccgccgcc gtcttctcac gatgtcgaac
     5161 cggcattctg atccatgtta ccagcatccc cgcttgtctc cgtcgttatc ctcaactata
     5221 atggccgtcg cttcaccgat acgcttttcg aatcgctccg gcgtaccacc tatccccatg
     5281 tcgaatgggt tatggtggat aatgcatcca gcgacgacag cgtatcctac acacgcgaaa
     5341 aatttccctg ggttcgcatc atagaaacag gcgctaatct cggctatacg ggcggcaata
     5401 acgtcggcat ccttcaaagt aaaggcgcct acgtggtact cctcaataac gacgtcgaag
     5461 ccgatccgga ttggctcacg catttggtca acgccgctga ggccgatgca tcgattgctg
     5521 cgctccagcc caaattgcaa tccatgatca acaaaggttt ttttgaatac gccggggcca
     5581 gcggtggttt tattgatccc tggggatatc cctttttgcg cggccggata tttgatacca
     5641 tcgaaaagga tcacggacaa tacgatgacg tgcgggatat tttctgggcc agcggtgcgg
     5701 ctttgtttct acgccgttcg gcgctggatg aaatcggctt gctggatgaa gactttttta
     5761 tgcactttga agagattgat ttatgctggc gattgcattg ggcaggttat cgcatacagg
     5821 ttattccgca aagccggata tatcattatg tcagcgcttc tctgcctgcg gccaatctgc
     5881 gcaagttgta ttggaatcac cgcaacagcc ttgtcgtatt gatcaaaaat ttacctcggc
     5941 gcagtttctg gcgaacccta ctttttcgtt ttgttctgga cggtatcgcg gccatccaaa
     6001 gcctttttcg cggtgagccg ctccgtatgt gggctgttct caaagcgcat ttttggattt
     6061 acgcgcattg tatttcattg tccagtaaac gtaagttcgc caaaaatctt cataaaaccg
     6121 acgaaacctc tttcgctcat ctcatatacc ctcgcagcat cgtagcggat tacttcttga
     6181 agaagaaacg cgaatttcat actatgggtt tttgattaag gataatggtt ttttcatgac
     6241 gatattgctt tggggcggaa gcggccagct gggtaaagac attcaacgtg tattagccga
     6301 tcagcatcac gttatagcga tcagccgttc ggaatgtgat atttccgatt ccgcacaggt
     6361 tctaagcgcc acagaaaaac atcgtccgga tgcggtcgtt aattgcgccg ccatgacgga
     6421 tgttcccggt tgcgaacaac gtacggagga agcttttacc gtcaacgcgc ttgccgcaag
     6481 gcatattgcg gatgccagcc gaattttgaa tgcctatttg atacacatca gcaccgatta
     6541 tgttttcgac ggtaataaaa aaactccgta tatcgaatcc gatcttacgg ctccggttaa
     6601 cacgtatggc atcaccaaac tcgccggcga tcattttgtg aacgcctatc atccggctgc
     6661 tgcagtggtg cgcacatcgg gattgtacgg gatattcgaa tgccgcggaa aaaagacaaa
     6721 ttttgttgag accatgcttc ggcttggccg tgaacgcgag atcgtaaaag tcgtggatga
     6781 cgaagtgctt acgccgacct acacacacga cttggctggg caaatcgccc tgatgttgaa
     6841 cgacaaaccg gcgggaattt ttcatgcgac taatgccggc gcttgttcgt ggtacgattt
     6901 tacgcaaact attttttcaa tagccggtta tacgacgcgc ttggagcgca cgacggtcgc
     6961 ggccttcggc agtcctgtcc gccgcccggc gtattcggtt ttagaaaatg ccgcattaca
     7021 aaaactcggc atagatcgta tgcgcccctg gcaagacgcc ttgcgcgctt atatgaccga
     7081 acgatccgac aaataatatt tattcattta taggagcgct aacacagaat gaaccgcttt
     7141 gcttttatca accgcataac tgccgcaatc gtatttgcgg tggctttcct tggctatctt
     7201 tttacgattt cgccgacact gtcgtattgg gattgcggcg aatttgccgc aacggcatac
     7261 actatggcta ttccccaccc gcccggctcg cctctgtttc ttttactggg tcgaattttt
     7321 tccattctgc cgacctccgg tatcggtcac gcattgggtt tagccgtcac cgattacgac
     7381 attgcttttc gcatcaatct gatttcaaca tttaccagtg ctttcgccgt attgtttttg
     7441 tatctgacga cagtacgcct gatcctacag tggaaagagc gcccggaatc aacgtttggt
     7501 tcgctgaaaa tcgtcatcgc ttcggccatc agtgcactta cgtttgcatt tacctatagc
     7561 caatggttta atgcggtcga atcggaagtc tatgcggctt cgattttttg tacggcgata
     7621 gtgacatggc tgatcacggt gtggctggaa aagccggatg atatccacag cgacgtatat
     7681 ctcctcctga tcgcctatat cctcgggctc gccataggta ttcacctttt ggtgattctt
     7741 gcgttgccat ttattttctt cctgatttat tccaaaaaat ttgaaatcac tttttccggt
     7801 tttttcaaat ttttactgat cggcctcatc gcgatgggcg tgatttacaa agtatttctt
     7861 ttctattcta ttcaaatccc ttttatgctg gatcgttttg gtctgagcac ggtttccgtg
     7921 tttctgatct ttgccgtgct gatttatctt tcgtttatca tgatccgcaa taataaccat
     7981 aatggcgcac tgatcgtcat ttcatcgcta ttgatttttc tcggatattc gacgtatgcg
     8041 acgatcatga ttcgctcagg actcaacccc aatgtggatc aaaatgaccc tgatacgtgg
     8101 agcaaattca tcagctacct caaccgtgag caatacggtg aacttgtgct gtggccgcgt
     8161 aacgcaccga tctgggaata tcagatcaaa aagatgtttg tccgttattt taactggcaa
     8221 ttcatcggtc gcccggacga atacgcgctg tccctcattg atcacctgcg caatgccatc
     8281 ggttggagtg tggataaatt gcaagacacg caagaagacc gttacagtta tatgtacacc
     8341 gtgttgagct tccgcggatt atatgccttg ccgttcctca tcggtatttt cggcatgatt
     8401 catcatttca gtaaagactg gaaacgtgca ctctcgacgt tgggactttt tatcatgacc
     8461 ggtattatga tcattgtgta tgtcaatcaa ccggatcctc agccacgcga acgtgactat
     8521 tcgtatgtcg gcgcgttctt tgcattttcg atttggatcg gcattggtat ttatgcgatc
     8581 tttgaaacaa tcgaagaaaa atttaaagac agcgacatga cgcgcatgct gacatacggc
     8641 gtaaccggtg ttgcctttgt actgctgccg ctcaacatgt ttgtatacaa caaattttct
     8701 tccagccgtc agggcaacta tgtggcctgg gattattcgt acaacttatt ggtgacatgc
     8761 gaaccaaatg ccattctcta cacgaacggc gataacgata ccttcccgct ttggtattta
     8821 caggaagtcg aaaaagtgcg ccccgatgta cgtatcgtca atctgagcct cgtcaatacg
     8881 gaatggtata tcaaccagat gaagaatctt gaaccgacat accacatgcc ggatggtacg
     8941 acattcaagg cgctcaaagt acctgtcgga tttagtgatc gcgaaatttt gggtgacccc
     9001 aaaattccca attcatccat ccagcccatg cgctgggacc gccgtgactt tgcgctcgat
     9061 gttcccaaag acgtgtactg gaaagattgg gtcgaatccg gccgcaagct tccccccggt
     9121 tacgatacga tgaccgttcc taaaatgaaa ttcaccgtcg aaccgaccat ccaaggtcgc
     9181 gggttacgcg tgcaggatct gatggtgctg gatattcttt ttgctaataa atttgaacgt
     9241 ccgatttatt ttgctatcac cgttagcaat gacaatatgt gcggtctgga taaatacctg
     9301 cgcatggatg gcctggcgat gaagcttgtc ggcgtgcccg gcaccgatat gtctattgat
     9361 cacatgtatg aaaacacatt caagaaatac aaataccgta acatgaacaa tccgaacgtg
     9421 gcatatgacg ataacgttcg gcgattgacg caaaactacc gcacgctgta cctgcgtatg
     9481 gccgaatact atcgtacgca aaaaaacaca tcatccggat tgcatacgca gattgatgac
     9541 gcgtatccgg cagaatttac gaccaatcaa aaaattgtcg cgattttaga ttcgatggaa
     9601 aaaaccattc cacagaatgt catccctgtg cgcgactacc gtctgaaact ggcgatcggt
     9661 cagttttatt cggatgccgg caaacctgaa aaattgcgtg aggccgctga agatattctc
     9721 gctaacgaga aagccgtgcg tctggacccg tccggtaaac tgcgtgtggc cgccatgttc
     9781 cttttcaacc ttaaggacgc ggaacgcgct aaaaaaatac tcgagccgat gtcgcaggcc
     9841 gatccgacca accccgaaat tctcgggtat tatacgatgg cgctcgaaca gcttggcgaa
     9901 tacgcgcaag cggctcaggt tttggaacag tgggtagcct tcaatccgaa agatgtacag
     9961 gcacaagcta agctcaatga gctgaaatcc aaaatcaaac cgtaagacca tgcctgttac
    10021 atggcaaaaa tacgtatgga cggttttaaa aatctccgta agcttgggct tgatgtattg
    10081 ggccgtacgg agtattgatt taacggtgat gatagcgatt tggcagtcca tcaatgttaa
    10141 ttggatatta gcgtcattta tacttcttac attgagttat ctgcttggtg catggcagtg
    10201 gcagagcata ttagcgatcg gcgatatccg attgaaatac cctacggtcg tagggtatta
    10261 ttatgtggga ctttttttta ataattttct cataagcggt atggggggcg atgtgttgcg
    10321 agtgtatgat attcaccgcc atacctccga tccgcaacgc ctttcaccgg ctttggccgt
    10381 cgtttttttt gatcgtttcg tgggcttatt gatccttatt ttatatgcgt gcatttccgg
    10441 tattttttta atcggtcaag gcgaatcctt gcgaatgttt tttgccatca ccgggctttt
    10501 actcctgtgg atattcgctt tgattcttct ttttcaccgc cctttggcca tatttctcct
    10561 gacaccgctg gcatggctcg tacctaaacg gttaagaatg cgggttgatc atctctacag
    10621 tgaattgaat cgttttcgta cggcaccgca gcttttgatg cgcgtttttc tgattgcagg
    10681 cggagtgcaa atgcttcgca tcatcgctat agcggccatc ggtcgggcgc tgggcgatgc
    10741 atcttcgttg atgttttaca ttctgtttgt acccatgatt tcactggccg ccagtttgcc
    10801 catttccatc ggcggcacgg gcccccgcga acaaaccacg gtatttcttt ttcgcaaaat
    10861 cggtgtttct tcagagatcg ctttttcgat cggtttcgtg acgtatcttt tatctgccgt
    10921 ttcaactttg cccggtggtt ttatttttat gctgagaaaa aataaagcgg aacatgccgt
    10981 cgtatcataa actgcttttc gttgtattat ttttaggaag ttgtaaaccg acacaatcag
    11041 aagccatcgg aggaacggat acgcttattc ttttttgtga cagtacgtta tcaccgatca
    11101 ttacggcttc acttcataaa acatggttta tgccgagagc tgagacggct tttgatgtac
    11161 agcatcaaac tatggatcaa ttcaccgttt accgtcattt ccgatacctg ctctttgccg
    11221 gatcgctcga cgctcatgat gctgtttcaa aatggatacg ctccaccctt tcgcccgatg
    11281 atgaaaaagc cgttcgcgaa ggtcaactat ggattctgga aaaagaatca ccctgggcgc
    11341 aaggacagat gatatgggtt ttatgcactc cggattacga aacaatggtc cgtcgctgtg
    11401 cgactgaagg aataatgctg catcggcgtt tggaagaaaa acgacgtgaa caggaaacgg
    11461 tcatgatgca acatgattat tttgacgcta tgcccgtcca aagcgcggac gccgatactt
    11521 tttatctata tgacggcttc tcacaacagc ctgcaccgat gtcgaaaact tttttttggt
    11581 acagatcaac tgaacgcttt caagaatgga ttttgtttag tacatttaca ggcgattcag
    11641 ttaacatatc aacatcgctc ggcaggcata ctcagaagtt actcaatgca ttgccggatt
    11701 ctttgaaata ccgcgatgac tttattacga gcgatacctt gtacacaaca tccggtcctt
    11761 tgttacgaat gtgtaccgtt tggactacaa aaaatcgcac taatacattt ggcggaccgc
    11821 tttggttata tgtgtcattg actccgacaa ataagccaaa aaaaataggc gtcgcaatgg
    11881 ctttcggacc tcaaaatgaa agaaaacatc cggctatgcg ccgattggaa aatttaataa
    11941 tttataatct tactcgttcg tatgaataac cgcattttta ccatctccaa tatcctcagc
    12001 atgctgcgaa tcgtgttcgc cgtacctata ctttattttt tacatcaggg catcgaagac
    12061 ccgacagcca acatctacgc attgatcact atattgatcg ccggttttac ggatttattt
    12121 gacggctatt tggcgcgccg attggaccaa gtcacggaat tggggaaaat cattgacccc
    12181 cttgccgaca aaatcgtttc ggcgattatt ttggtttatc ttgctctgat caggcccgat
    12241 tttcccttgt ggttgctgat tgttgcgatc gtacgcgatg tggtaatttt tgcggcggcc
    12301 atgtacgtca aacaaaaata caactatctt tttacttcca atatgctcgg aaaatgggcg
    12361 tttacggtac tggggcttac ttttgccatt ttcgtggtgc gaaataattt tagtatcgaa
    12421 tggcttttcc agtcgttcct ctggcttaca tttattatgc taattttatc tttttacgtt
    12481 tacgcgcagc gtttatgggt attcatgcaa aaccaacgga aaaacgcatg aaatatttta
    12541 ttattacttt attttgtttg ctgacatttg gcgaactgtc ctacgctcag gtacgcggcg
    12601 gaaatcgaaa tcgtatcgat aaaaaaaaca ccgttgccga tgatccgcaa gtagcttttc
    12661 gaatggcttt taaggcttac aaagaaaaag atattcccaa agctcaaaaa ttattaaaaa
    12721 acacattaga caaacatcca acgcatgtat tgagtcttat tctgatttct aaaattttgg
    12781 tggaacagga tgatctgaaa tcagcaaaaa aattcattaa aaccatccct gaatccgaac
    12841 accaccgtcc tgcgatagcc cgtatattgg ctgaaattgc ttatcgcaac ggtaagccgc
    12901 ttaaggcttt acaaataatc aatcggactc aatcggatgc cgcatcgtcg gacgatgcgg
    12961 agttttttga taaccccaat caagatatcg catcgtttaa gaagcaactt ctagcctcta
    13021 cacgacaaag ccaacgcaag gaaatgccat acgcaaaaga aaaaccggca acggaaaccc
    13081 atcttacgat ccccggcgac ttgcctacgg ttgcgaccga acgtcgttta cgactggcta
    13141 ttttttcttt atctgttgca ccttctttgg atgacacgac actcggatct tcgacggcgt
    13201 cgtacctcac tacagctgcc gtgcaaacgc aatgttttag agtggtcgaa cgtgaaaatt
    13261 tagaaaaagt tatgatcgaa caggattttc aaatgacgga cgccgttgat actgaaacag
    13321 cggttgaaac cggttatatc atgggtgtag atgcaatcct tgtaggaagt gtacgcccga
    13381 tggcgcaagg ttacgaagta gatggacgct tgattcgtgt ggaaaccggc gaaataattg
    13441 cggcgacaaa tcaaagtatt tcatccgtat cgctggtacg acaatcggct gccaatctta
    13501 tgtttgactt gacccgtgcg gcgtataaat aatttgttgc ttgtatcggg taaattaatt
    13561 atataattaa tgtcacgcca caaacggttc tgcgtttgcg aatacattta acctttcaca
    13621 aaaaatatga tcgttgaaaa aatccgggaa ttggcggaag atttgcgtcg tacgcatcaa
    13681 gatttgcgcg ttatgcagtc ggatatgcgt atgcaggatg gcgcgtacgc gcgcgaagca
    13741 gacattgacc gtgtcatctc cgacctcacc acattcaagt tgaatgtgac atatctgcgc
    13801 cttaaagccg gcgaactcgt tcagcggaat aatcttgagt tctcagaagt gcgcgaagaa
    13861 gtgcgttact ttgcaaccta cctcaaacat gcctccgaca catttgatga aatctgtaac
    13921 agtatggcct acggaggtcg ttccggcgat gatgccgcca tgcaaaaaac cggcgaagaa
    13981 ttactcatcc tgcaacgcca ccttcaggaa atgaacgagc accttttagt cattaattcg
    14041 aaattaggaa agacttcata acttttatgt acgaaaaacc catgaaacag agcaaaaaag
    14101 tacaaatcgc aagcgaaagc gccgtcaaag tgaccaccca tcgtattacg gacgacttag
    14161 ccagcgtgga aattgacgac aagatgatga cggaagagca catgaaaatg ctcgaaaagg
    14221 ccgtccatga attgcttgcc gaaaattatc actcgatcat attggatctt accaaactca
    14281 aacgtatcaa cagtaccggg cttggtaaat ttattaacct ctacaaagcc gcagcggata
    14341 aagacggcgt catgaaaatc ggtggtgttt ctgactttgt acgcaatgta cttacgagta
    14401 ccaaacttaa cgagattttc gaaattcata atacactgga cgatgccatc aaaagttttc
    14461 agacatccgg cgcataagac gctcttaaat tattattcca aaaaaacgcg gggtttatac
    14521 tccgcgtttt ttatttgaaa tattttcaga aaaacctttg ctatcgtttc aaaaaatatt
    14581 atttgaattt gtatcttcat tttatatctt tgcaccgctt tttatatatg ttcaaagggc
    14641 cgttagctca gcggttagag caggggactc ataatccctt ggtccggggt tcaaatccct
    14701 gacggcccac cataaaacct taagttataa acttagggtt ttattatttg tgcaaccggt
    14761 ttaaccactc ccttcccata tatattacaa aaccgcatta acatgtaggc catttttatt
    14821 ttgtattatt ctattatttt ataattatca aatcaaatcg gagaaaatat gcgttttagc
    14881 caaattgtat cgttgttttt aattgttagt tttgtaagtt ttaccgcatg cgactctgat
    14941 agtaagtcaa cggtagacgc ttccgaacaa aaacccgttc tttcgctcac ccaacatgat
    15001 tccacgattt taactcaggc ttatgtcaac agctcatggt atgaatttgg tccgcgcctt
    15061 cgttttttaa aaaacgggac gatcacaggg cttggtatga tgatgcccga ttccggttca
    15121 tacgaagtta cgctttggga tttatcggat acgagcattg tcactacagt gacgatcgct
    15181 gcggactccg gagagctaaa atacgttgca attgatccta tccaagtgca gaccgacgag
    15241 cgttattcgg ttaccatttt atccgatgat tggtattggt ttgaagacat ctcaggcaaa
    15301 aataattttt tacctcagac attcgatgac gtaatgattg aagattacgc ttacatatcc
    15361 ggctcgacgc accagttccc tactgacgcg tttaccgatt atttggctgg gattcaggat
    15421 attatttttg ttccggagaa atagtcaaaa tttatttacc tcccggcgtt ctacagttta
    15481 cagatcagat tcaaattcga tgaaaataaa taggcatgaa taagaaaacc ccttaagccg
    15541 atcggactta aggggtttat taataaaatt tagatgctac taaataacag cgattacctt
    15601 ataaaagatt ctttttgcta aacgtgatcg gcagtgttac ggccactttc accggtttgt
    15661 ttttaaacgt agccggttca aatttgcact ttagcacaaa atctatggcc gcttgaccga
    15721 aaccaacatt accgtcttcc tttactactt cggctttgac cacgtttcct ttttcatcca
    15781 cgacagcacg aactaacgcc accccttcga tacccgcttg ttgcgcggct tccgggtact
    15841 tcaggttacg tttaagaaaa tcagctccgc ctttgagaag cgggcgtgta tcgtaaactg
    15901 taaatacgtc gtcatcgcct ttttcaactt ttttaaacgg tggtggtggt ggcggtggtg
    15961 tttcatccag atcaatatcc gtgtcatcta tggtctcatc gccgaggagc gtttcctggt
    16021 ctgtagcaac cggcacaacc gggcggcgcg gtggtggtgc atgatgcgaa gccgtaagat
    16081 ccacgcgtgt taactcaatc tgtgtcggcg tacgtatctc agcttttgca tattcggtaa
    16141 tcgtcacggt atgtaaaata atatatgcaa gaacgatggt tatgatgaca gaaatatcca
    16201 tcatcaacgt ataacgatta tttcgtgatt gatactgcat agcttaataa attgcgttag
    16261 aacgtgaatc ggtcccgtag taaatccgga gcgctccggc acgacgcaac tgttcttgca
    16321 cgtccgccaa agcgcccatc gtcaacgatt cgtccacacg tagaaagata agcattttgt
    16381 gatcggcttc gattttcgcg cgggctaccg aatataactc ttccatcgaa ttaatcggat
    16441 aatcatcaaa tacaatacgc tgttgtttgt caatccaaag gtaagatgta tgcgttttcg
    16501 tggataattt ttcgatatta aatgcagccg ggacttgcac aggcaagccg ctgtattttt
    16561 tgatcacggt agataccata aagaacaata gaagcgaaaa aacaatgtcg gacatcgaag
    16621 cgctcgatac cggcaaagat tctatagggc gacgtcgaaa cttcatatca gcgatcaggc
    16681 tccaaaactg aaattttacg gacaccggct tctttgagtt gatccatcac ggcaataaaa
    16741 ttgcgatacg gcgtttttcg cgtcgttttt accgtgacaa ccaatcggtc gttttcaccg
    16801 gcgacatttt ttacaagcga ctgaacttg
//
LOCUS       DAOQGF010000074        16321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1626_length_16321_cov_6.711238, whole genome shotgun sequence.
ACCESSION   DAOQGF010000074 DAOQGF010000000
VERSION     DAOQGF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_16321_cov_6.711238"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1336
                     /locus_tag="PK937_10610"
     CDS             146..1336
                     /locus_tag="PK937_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33066.1"
                     /translation="MIYLKDVFKNILLSACIVAACLTCDDKDKDNPPKPPVDDIITYE
                     KILGTNASEYVRGGDLTNDGGCIITGDQIIDGDFWAYLMRTNNTGDTLWMRRYRAPGN
                     LYTSYGLGLAVQQTNDGGFIMVGDTQPDTNKPGDSDFYIVKTDANGDTLWTRFYDRSR
                     TDGATSVKQTSDGGYIISGNSARYLPDGGGVAYVFRLTSDGSWVWGRTIDEYGTTYAV
                     EVNEDGAYMITGDGAIVTKIDPSGNTIWSKFYGRPFGNTLFAIDKTNDGGYVFAGKTE
                     PASGALPVAYLVRTDVKGDTLWTRSYPYLYSGDMRCVSSLPDGGFLMSGNTEKADVIR
                     TDSNGDTLWTRSFGDDLGGAGFIMGKPTEDGGFIFAGGIRLNRRDIYLVKTDSLGFVK
                     SKNQ"
     gene            1392..2795
                     /locus_tag="PK937_10615"
     CDS             1392..2795
                     /locus_tag="PK937_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH33067.1"
                     /translation="MNALYTLMICFLFWSFATAQDLFYYGKGQKIPLSLSDKTLTIKF
                     KTDVSESQMNDLKSSDPDIDKIEKMASRGLHKVSLKNDADIQGIISRLQINPKVERIN
                     PAYKLGEMEMLISDLFRVQFNTGITRAEIDALNEEFGVEIVEELTARPGVFTLRVKSS
                     IASVLDITNRYYEDPNTEWAVPKFFHRWSLHSIPNDTYFASQYYLHNTGQTGGVADAD
                     INAPEAWDITLGSPSIVVAVIDQGVSTGNDPLKPAHEDLPQSRIVRLAGSDFYGSGNG
                     PDENPDPEGNQAHGFAVAGIIAASHNNGTGIAGIAPECKIMPIRMFGARIYDDIYEPL
                     SASDFADAIDFARVNNADVISNSWGNDVDGYWNDDIASAISRALTQGRNGKGCVVVQS
                     AGNNASRSDGYYGFVEFPANVNGVIAVGATDKSNNILYYSPRSKLSQKDSIDVVCPTG
                     QLPSLVLLYNCDNKFIA"
     gene            2802..4043
                     /locus_tag="PK937_10620"
     CDS             2802..4043
                     /locus_tag="PK937_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNH33068.1"
                     /translation="MEGDVWSLDIPGNAGYNNGLYRISSPYCYDKYTSLPPGYEGDGS
                     PYNYMRRFGGTSAACPQVSGVAALILSLNSNFTQSQVQYFIRNSAVDMGTAGWDVDFG
                     FGRLNAHKALVLAGFPIIADPITNIHWCYNSQKKISWTSNLSTSYTVKVSTDGGSTFP
                     TTIASNVTSGSASYSYNWTVNIGPSFTCKLLVIDNNNSALRDTSDLFVIDGTAVPSIP
                     GSFSLTSKGGHPWLTWTTDGSAAGYDLYRASSEYNPPYTWYKFMTINDPTITNYHDVV
                     VDIGITNPNTMKYKINAFNGCNMYSVFTAEKQINYDQFMKNNNANETLIPNEFSLNQN
                     YPNPFNPTTTIEYAVAKTTLVRLTVYDELGREVAILVNEKKMPGIYRVDFDASKFASG
                     VYFYRMTAGEFMETKKLLLVK"
     gene            4256..5155
                     /locus_tag="PK937_10625"
     CDS             4256..5155
                     /locus_tag="PK937_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33069.1"
                     /translation="MFFPLLESLLHRDIATSKTRIREFLTTDESPRLLYKAFLTIAAQ
                     NAPSDNLHDDAVIKTSAYVAVMEGIDKNKIAHITDALVEYLCTMPIEPIDTSVLKPEF
                     ASHDVVSVHDLEEALDAGDEKLVFEKIRDLLATMDNKQYFMEIMILFGLKRSLNTALA
                     AVHTSMAIQVMDWKNNFTPFLIHRLVRVVCNDKNRETPAKTDRAVDWDDLFRFAPDTY
                     GLIKICALWRSIHEAGVLRSKTEPWVAHYALRLLDTFPKTTDFPVLDQVLSAEDTIRI
                     KGALQGLPHDSARALDSKIPFVV"
     gene            5201..5734
                     /gene="def"
                     /locus_tag="PK937_10630"
     CDS             5201..5734
                     /gene="def"
                     /locus_tag="PK937_10630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNH33070.1"
                     /translation="MATLKIVTYGNPILREKTKPVKKIDATIKTLVRNMIETMHKADG
                     IGLAAPQIGKSLALFVVDISPIEEGHQPMVFMNVEILSSKGNAPYKEGCLSIPGVYAD
                     VHRPEKIKLRYMDMDGKTHEVAADGLLARVIQHENDHINGKLFIDYLDEEMLAPLRPE
                     IEAIEAKYKKPTVAKKK"
     gene            5734..6579
                     /locus_tag="PK937_10635"
     CDS             5734..6579
                     /locus_tag="PK937_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH33071.1"
                     /translation="MERKNFKIFNKRREPIRGDVCIPDYNRRYPVIVLCHGFKGFKDW
                     GFFPFLSELLCKKGFIVVKFNFSGSGVGEDMQNFTELDRFASNTYSKELEDLERVLDE
                     LEKGNICEKFGYFDKIGLLGHSRGGGITILKAAVDKRIKALVTWSSIATVERHSFMDV
                     LPLWKRQKFIEVPNTRTGQMMRLNLDVVDDIEQNSKTRLNIVKSAARLDAPTMLIHGE
                     NDESVPVNESRQIFDQLHLSRSRIEIIPQATHTYGAVHPFTGPTPELKKAISLTQEWF
                     TNFLK"
     gene            6581..7225
                     /locus_tag="PK937_10640"
     CDS             6581..7225
                     /locus_tag="PK937_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH33072.1"
                     /translation="MILQSLTVGPFQENCYIIGDPATREGAVIDPGDEAPRIIKAMEN
                     TGLRFKYIINTHAHIDHVGAVQSVKDHFAIPFYLHRDDEVILRHMPQSAAMFGLKVGR
                     VPDIEFYFNPPADMRLGDLSFKIFHAPGHSPGSVCFYFAMQHILIAGDVLFNGSIGRT
                     DLPGGDFDTLINSIRQNIFTLPEETTVYPGHGPETTVGFEKKFNPFFDHNGLMA"
     gene            7250..9610
                     /locus_tag="PK937_10645"
     CDS             7250..9610
                     /locus_tag="PK937_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH33073.1"
                     /translation="MLRVLLIVFYVGFYSTWAAQSDVLSKTTADSVNYYLLHPVLTNQ
                     AYEVSNYTLTRDRAKFVLKNGFIYTAKPFSGNTIMALFLGTGEFELTPPGDIEQKNLT
                     RFLGTSTLSKEFTNLFFVFTDSTDQWIYRNEPATLTEDEFKNAARFCESAVKYLADPF
                     GEYVDQHTELMNDLVNRRKQGIYLSVFSTLSANDLGSSTTILSKWRDEPLIYKIDPTA
                     NDGEHVTLGRRIRNQGKGFYQEIVSKFPMIPDNSVPYFLRTERKNDFKISDYNMRMTI
                     NENLSSQTSAQLTMRLNNPVNWIRLFLYYRLHVDSVKINGRHVPFHAPVAKDDGFERF
                     ESSPIWINTASVAEQTEPIRVEIFYHGKFLEREGDYVYFKATDDWYPAGFTGRHDRFS
                     FDITYTYPSTYMLVSHGTEREIEKNKKTITVQWITRQPTRHVTFNMGVYKDIRFKDER
                     IPDIHMVFRYDRNGKDVKKQVMADVANSYMFFQEKFGSLPIGKLVAAEYSYSHSEAYD
                     GLVNISASVFNSNDDDGVDEWIRAHEVAHQWWGIGVDFDSYHDQWLSEAFAEYSGMLY
                     VQQSLSDNKEFFRFLNITKERIFKNRKGIFVDGQEAGPISLGYRTSSSSTRGDYGLVI
                     YSKGMWVLHMLRNMMLDLNTMNEDRFFAMMKDYFETYYGKKATTDDFKRIVEKHVGTD
                     MAWFFDQWIHGTDLPKYEFAYTTKQTENGKFKVRCRIRQENAGENFQMFIPITIDFGD
                     NRKARMRLLVKGPQSEIDLPLLPLEPKKIIFNDFESVLCEVNYSNW"
     gene            9630..10166
                     /locus_tag="PK937_10650"
     CDS             9630..10166
                     /locus_tag="PK937_10650"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNH33074.1"
                     /translation="MSETIAPVFKPADPSPVKRLVSKRDIGISAFELENGNVMLEATF
                     LDPYHLLRLNIQIDPHTRYIVSSKMEMANHPHSACPGVTEKAKLLIGLQVTRGITKEI
                     SRRIGGSDGCVHLRELALETINFAATVMVGYDEGFGLMSREFNILDEHKRYDISKSIL
                     RNTCHIYKQPRTQEGETV"
     gene            10163..11029
                     /locus_tag="PK937_10655"
     CDS             10163..11029
                     /locus_tag="PK937_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH33075.1"
                     /translation="MKVDTKIGAYHLHPVETGRFALDGGAMFGVVPKNLWNKTNPADE
                     QNRITLAMRCLLLVSDDGKKILIDNGVGHKYNEKFASMYRIDHSVYTLERSLQKYDLT
                     PNDITDVILTHLHFDHAGGSTKMDEQSRIVAAFPNAKYYVQQKHYEWGEKASEKDRAS
                     FLKPDYEPLVQSGQLQLIEQTDGIFPNITFFIANGHSPYQQLPIIHDAQHTVFFCGDL
                     IPTTTHIAVPYVMAYDNQPLVTIAEKKDILTRAFENRWILFFEHDPETAMSGVRRDEK
                     GFRMGDIVAVKG"
     gene            11031..11723
                     /gene="ispD"
                     /locus_tag="PK937_10660"
     CDS             11031..11723
                     /gene="ispD"
                     /locus_tag="PK937_10660"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNH33076.1"
                     /translation="MIKGKKVTAILPAAGIGKRMGVGSKKQFLEVHGKPILVHTLEKF
                     HHAIYVDEVILVLQNEWIPFVQEEIIGPYGFSRIHTLVPGGTERQDSVYEGLKAVEES
                     DIVLVHDAVRPCVRADKIDELIEITVEYSAAILAVRPKDTIKQQDANGFVARTIDRNT
                     LWNVQTPQAFQYSLLRSAFDKAYAENYYGTDEAMLIERIGHAVKIVEGHYDNIKITTP
                     EDLALAERFLKH"
     gene            11819..12331
                     /locus_tag="PK937_10665"
     CDS             11819..12331
                     /locus_tag="PK937_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33077.1"
                     /translation="MNEEYKQHMDKGREAFVAKDFRKSFEYYNAALNLVKDLKDHDDT
                     KQHYFLTQECVFDALEMMQDYKQAIQRIDEVIAEEQAKGDTTTKHMAHFMQRKACLEI
                     KSGNNRPGVKLAGEAYELGWKTKEQKTNYEIAAQIWNEHQRGSKITTEWVEYWQKHFN
                     YWVADKELAI"
     gene            12482..13621
                     /locus_tag="PK937_10670"
     CDS             12482..13621
                     /locus_tag="PK937_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNH33078.1"
                     /translation="MDFKLSEEQEMIRQTARDFAQEHLAPHAAERDEKQFFPKDVIKQ
                     MGELGFMGMMVNDKWGGAGLDTISYVHVIMELAKADASAAVIASVNNSLVCFNIEKYG
                     TDAQKEKYLKPLATGGALGAYSLSEAVSGSDAAGLICFAENKGDHYIVNGTKLWVTNG
                     YHSDYVVCFIRTDKSNKTKGISAFVVDKSFPGFKVGKKENKLGIRASDTTELVFENCK
                     VPKENLLGQEGLGFKIAMETLDGGRIGIAAQAIGIAEAALEETVKYTKAREQFGQPIA
                     KFQANEFKIADMAMRIDAAKLLLFRACTLKDQGQKFGKEAAMAKLFASETAMWVTTQA
                     VQMHGGYGYTKEYPVERFMRDAKITEIYEGTSEIQRVVIARNVLG"
     gene            13652..14755
                     /locus_tag="PK937_10675"
     CDS             13652..14755
                     /locus_tag="PK937_10675"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNH33079.1"
                     /translation="MRTFEMIEEKEHEQVVFFSDKASGLRAIVAIHDTTLGPALGGCR
                     MYPYATTEEALNDVLRLSRGMSYKAAVAGLNLGGGKAVIIGDPKKHKNEILFRTFGRF
                     IQGLGGRYITAEDVGTSVTDMEWIRMETRYVTGISRAQGGSGDPSPVTALGTYRGLKA
                     CVEKVFGTQSLKGLRIAVQGLGHVGYYLVRHLHDEGAKLVVTDVDGERVKRVVSEFGA
                     EYVDSDKIYDADVDVFSPCAMGAIINDDTIGRLKCKIIAGAANNQLQDEARHGAMLLA
                     KKITYAPDYVINAGGLINVYNELEGYNQEKALAQAKGICDIMKKILELAERENISTHE
                     ASNRIAEERIRQIGHIKSHYVTQPESVLKRIER"
     gene            14794..15291
                     /gene="nusB"
                     /locus_tag="PK937_10680"
     CDS             14794..15291
                     /gene="nusB"
                     /locus_tag="PK937_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNH33080.1"
                     /translation="MNPLLADDGAKPGKNTAPKNSRREARETVLKALYAYEMTQDNIK
                     RVLDDVCDNFTEPATQFIHTLVQQTIAHQMEMDVLIRARAQNWEFERIALIDRILMRM
                     ALCEILFFEDIPPKVSINEMIEISKRYSTDKSSKFINGILDSIYEDLKNVGRITKTGR
                     GIIES"
     gene            15350..15949
                     /locus_tag="PK937_10685"
     CDS             15350..15949
                     /locus_tag="PK937_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HNH33081.1"
                     /translation="MISVAKILPISEVNGPGKRCVVWVQGCPKRCPGCWNPEFLSFEK
                     STNTVSAKELVTMISKATHGFRDIEGVTFSGGEPFAYAECLTEVAQLLRKQNLTIMSY
                     SGFTVEEIRTKGNFYVSLLEALDILVDGEYKRELNVNRLWRSSANQRVLFLTDRYIAY
                     QAHIDEEYRELEFTLNTNQITTTGFPEVSILKNLTNHRE"
     gene            15956..>16321
                     /locus_tag="PK937_10690"
     CDS             15956..>16321
                     /locus_tag="PK937_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33082.1"
                     /translation="MAFKNLVILTMIAGSIFAQQKNIKEPFGMVISLRANIEAKGDKA
                     IKQGDLLYKGDVIDLGQGDYCKVFWADGAVKEYNGKFNVPDLKQLRKEILAMFAEQSA
                     TQSWFFKAQSHYSKQIYRSD"
BASE COUNT         4832 a         3290 c         3763 g         4436 t
ORIGIN      
        1 gggtgaataa aaaaaggaaa gagaatatta ccggattcag accaatgcag atttgaatta
       61 ttatggtact attgattttc ccggtgataa tgttaaacaa taccccggcc ttatcaatcg
      121 gatttccgga aaggaggaag cttttttgat ttacctaaag gatgttttta agaatattct
      181 tctttcagcg tgtatcgtgg ccgcatgcct cacctgcgat gataaagata aggataaccc
      241 gcccaaaccg ccggtggacg atatcataac ttatgaaaaa atactcggga caaatgcatc
      301 ggaatatgta aggggggggg atctcacgaa tgacggcgga tgtattatta ccggggatca
      361 gatcattgat ggagatttct gggcctacct gatgcgaacg aataatacgg gcgacaccct
      421 gtggatgaga cgttaccgtg cgcccggcaa cctatacaca agttatggtc tagggcttgc
      481 agttcagcaa actaatgacg gcggttttat catggttgga gacactcaac cggatacgaa
      541 caagcccggg gatagtgact tctacatcgt aaaaaccgat gccaatggcg ataccctctg
      601 gacacgattc tatgacagat caaggacaga cggagccaca tcggtgaaac aaacatcgga
      661 cgggggatac attatttccg gtaattctgc acgatatttg cccgatggcg gcggcgtcgc
      721 ttatgtgttt cgattgacat cggatggttc gtgggtttgg ggcaggacta ttgacgagta
      781 tggaacaaca tatgcggtcg aagtgaatga ggacggagca tatatgataa cgggagatgg
      841 cgccatcgta acaaaaatag atccatccgg taatacaatt tggtcaaaat tctatggaag
      901 gccattcggt aatacgctat ttgccattga taaaaccaac gacggcgggt atgtgtttgc
      961 cggaaagacc gagccggcat cgggtgccct gccggttgcc tatcttgttc gaacggatgt
     1021 caaaggcgat acgctttgga cccgttcata tccttacctc tatagcggcg atatgcgctg
     1081 tgtaagcagt ttgcctgatg gaggattcct tatgtccgga aatactgaaa aagctgatgt
     1141 catcagaacc gattcaaatg gggatacctt gtggacccga agttttgggg atgatttagg
     1201 cggcgccgga tttatcatgg gtaaacccac agaggatggc gggtttattt tcgcaggcgg
     1261 cataagactc aacagaaggg acatttattt agtaaaaacg gattctttag ggtttgttaa
     1321 gtcaaagaat caataagtat ttttaatcaa ctgttctgaa acaaattact ctctaacggt
     1381 ggagattggt tatgaatgcg ttatacactc ttatgatttg ttttttgttt tggtcttttg
     1441 cgacggcaca agaccttttt tattatggca agggacaaaa aatccctcta tcactttcag
     1501 ataaaacact gacgattaaa ttcaaaaccg acgtgtcaga aagccaaatg aacgatctca
     1561 agtcttccga tcccgatatt gataaaatag agaaaatggc atcgcgtggt ttacataaag
     1621 tttcgttgaa aaacgacgcc gatattcagg ggataatatc gcggctgcaa attaatccga
     1681 aagtcgaaag aatcaacccg gcttacaaac tcggcgaaat ggagatgctc atttcagatc
     1741 tttttcgagt tcagttcaat accggcatca cacgcgcaga aatagatgcg cttaatgaag
     1801 aatttggcgt agaaatcgtg gaggaactta cggcccggcc cggcgtcttt acgctccgcg
     1861 tcaaatcttc gatcgcttcc gttttggata ttacaaaccg atactacgaa gaccctaata
     1921 cagaatgggc tgttccgaaa ttctttcatc ggtggagtct gcatagcata cccaatgaca
     1981 cctattttgc cagtcaatac tatctccaca ataccggcca gaccggcggt gtcgccgatg
     2041 cagacattaa tgcgccggaa gcctgggata ttacgttggg cagtccctct atcgtggttg
     2101 cagttattga tcagggtgtt tctacaggta acgatcccct caaacctgca catgaagatt
     2161 tacctcagag ccgaatagtg cgcttagccg gctcggattt ctatgggagc ggaaatggtc
     2221 ctgacgaaaa tcctgatccc gaaggtaatc aagcgcacgg atttgccgtg gcagggatta
     2281 tcgcggcgag ccataataac ggaacgggga tcgccggtat cgcgcctgag tgcaaaatca
     2341 tgcccattcg catgttcgga gcgcggattt atgatgatat ttacgagcct cttagcgctt
     2401 ctgattttgc cgatgcgatt gactttgccc gagtaaataa cgcggacgta atcagtaata
     2461 gctggggaaa cgatgtggat ggctattgga atgatgacat tgccagcgcg atttctagag
     2521 cgctgacaca gggtcgcaat ggcaaagggt gtgtcgtggt ccagtccgcc ggaaataatg
     2581 cgtctcgttc ggatggctat tatggttttg tagagttccc tgctaatgtc aatggagtga
     2641 tagccgtagg agctacagat aaatcaaaca atattttata ttacagtccg cgttccaagc
     2701 tcagtcaaaa agattctatt gatgttgttt gtcctaccgg gcaattacct tcacttgtgt
     2761 tactctataa ttgtgataac aaattcattg catgatccgg catggaaggt gacgtttgga
     2821 gtttagatat acccggaaat gcgggttata acaatggact gtatagaatc agttctccgt
     2881 attgctatga taaatacaca tcgcttcctc cgggctatga aggcgacgga tctccgtata
     2941 attatatgag acgatttggc ggaacttcag ccgcttgtcc gcaggtttcc ggtgtggctg
     3001 cgctgatttt gtcattgaac tctaatttta ctcaaagtca agttcagtat tttatcagaa
     3061 attccgctgt agatatggga acagccggtt gggacgttga ctttgggttt ggtcgtttga
     3121 atgcacacaa agccttagtt ttagccggtt ttccgatcat agcggatcct ataaccaata
     3181 ttcattggtg ctataattcg caaaaaaaaa tctcatggac aagtaatctt tcaacatctt
     3241 atacggttaa agtatcaaca gacggcggca gcacttttcc gacgactata gcgtctaatg
     3301 ttacatccgg aagcgcttca tattcttaca attggacagt gaatatcgga ccatctttta
     3361 cgtgtaaact actcgttatt gacaataata actctgcttt gagagatacc agcgatctct
     3421 ttgtaattga tgggacagcg gttccatcta ttcccggttc attttcattg acaagtaaag
     3481 gtggtcaccc ttggcttaca tggacaacgg acggttctgc ggccggatat gatttatatc
     3541 gggcatcgtc tgaatacaat cctccttaca catggtataa gtttatgacc ataaacgacc
     3601 cgacgattac caattatcat gatgttgtcg ttgatatagg tattacaaac cccaacacca
     3661 tgaaatataa gataaacgcc tttaatggat gcaatatgta ttctgtgttc acagcagaga
     3721 agcagataaa ttatgatcag ttcatgaaaa acaataatgc aaacgaaact ctgataccga
     3781 atgaattttc tttaaatcag aactatccta atccatttaa tccgacgaca accatagaat
     3841 acgccgttgc taaaacgacg cttgttcgat taacggtata tgatgagttg ggacgggaag
     3901 tggctatatt ggttaatgag aagaaaatgc cgggaatata tcgcgttgac tttgatgctt
     3961 caaaatttgc atccggtgta tatttctacc gtatgacagc aggtgaattt atggaaacaa
     4021 aaaaactttt gttggtgaag taagtattcg cgatgcgagc taaaccgatc tgagtttgaa
     4081 attctaagct tcatcaagtt atgaacaaaa acctgttact ctttacgagt aacaggtttt
     4141 tatctgcgtt tttaaaattt ggctctttcg ttaatcatct ggaaacggta acttaagtga
     4201 aaagaaaatg aacacctcat catcgaggtg tgagatataa aaaaatttaa caataatgtt
     4261 ttttccgctt cttgaatcgc tgctccatcg tgacattgca acatccaaaa ctcgtatacg
     4321 ggaatttctc actactgacg aatcaccacg cctattatat aaagcatttt tgacgatcgc
     4381 cgcgcaaaac gcgccctccg ataatttgca cgacgacgcc gtcatcaaaa ccagtgcata
     4441 cgttgccgtt atggaaggaa tagataaaaa taagatcgcc catataacgg atgcgcttgt
     4501 ggaatacctc tgtaccatgc cgattgaacc tattgacaca tccgtattaa aacccgaatt
     4561 tgcaagccat gatgtcgtat cggtgcatga tttggaagag gctttggatg ccggcgatga
     4621 aaagctcgta tttgaaaaaa tacgcgactt actggcgacg atggataata agcagtattt
     4681 catggaaatc atgattcttt tcggcctcaa gcgttccctg aatacggcac ttgcagccgt
     4741 gcacacctct atggcgatac aggtcatgga ttggaaaaat aattttacac catttctgat
     4801 tcatcgcttg gtacgcgtcg tatgcaatga taaaaacaga gaaacgccgg caaaaacaga
     4861 tcgtgctgtg gattgggatg acttatttcg atttgcaccg gatacctatg gccttattaa
     4921 aatctgcgcc ttatggcgta gtattcacga agccggtgta ctccgcagta aaacagagcc
     4981 gtgggttgcc cactatgcgc tccggctttt ggatacgttt cctaaaacca cggactttcc
     5041 tgttttagat caagtgctgt cggcagagga cacgattcgc attaaaggtg cattgcaagg
     5101 cctaccgcac gactccgcgc gtgcgcttga ttccaaaatt ccgtttgtag tttaagcgtt
     5161 tttttataca ttccgcccgt ataaatgata taccaatttt atggccacat taaaaatagt
     5221 aacctacggt aatccgatct tacgggaaaa aaccaaaccc gtaaaaaaaa tagatgcgac
     5281 gataaaaact ttggttcgca atatgatcga gactatgcat aaagcagatg ggatcggact
     5341 ggccgctccg caaatcggta aatcgctggc gctttttgtg gttgatattt cgccgatcga
     5401 agagggtcat cagccgatgg tattcatgaa tgtcgaaata ttgagttcga aaggcaatgc
     5461 gccgtacaaa gaaggatgtc tcagcattcc cggcgtgtat gccgacgtcc atcgtcctga
     5521 aaaaatcaaa ttgcgttata tggatatgga tggaaaaact cacgaagtgg cggctgacgg
     5581 gcttttagca cgagtcattc agcatgaaaa cgatcatatt aacggcaaat tgtttattga
     5641 ttatctggac gaagaaatgc tggcgccatt gcgcccggaa atcgaagcca tcgaagcgaa
     5701 gtataaaaaa cctaccgtag cgaagaaaaa ataatggaac gtaagaattt taagattttt
     5761 aataaacggc gcgagccgat ccgcggcgat gtttgtattc cggattataa tcgccgttat
     5821 ccggtgatcg tgttgtgcca cggcttcaag ggctttaaag actggggttt ttttccgttt
     5881 ctatcagaat tgctttgcaa aaaaggattt atcgtcgtga aattcaattt ctccggcagc
     5941 ggtgtcgggg aagatatgca aaattttacg gaattggatc gttttgcaag taatacctat
     6001 tccaaagaat tggaagacct tgagcgcgta ctggacgaat tggaaaaagg caatatttgc
     6061 gaaaaattcg gttactttga taaaatcggc ttacttggcc atagccgcgg cggcggtatt
     6121 accatattaa aagccgcggt ggacaaacga atcaaagcac tcgttacctg gtcatccatt
     6181 gctacggttg agcggcattc gtttatggat gttttgccgt tatggaaacg gcagaaattt
     6241 atcgaagtac ccaatacacg caccggacaa atgatgcgtc ttaatctgga tgtcgtcgat
     6301 gatattgagc agaacagcaa aacgcgcctc aacatagtca aatcggctgc tcgtttggat
     6361 gcacctacaa tgctgattca tggcgagaat gatgaaagcg tgccggtcaa tgaatcacgt
     6421 cagattttcg atcaattaca tctttcccgc tctcgcatag aaatcatacc gcaagctacg
     6481 catacctatg gcgcggtgca tccttttaca ggtccgacgc ccgagttgaa aaaagctatt
     6541 agtctcacgc aggagtggtt caccaatttc ctcaaataac atgatcctcc aatcgctcac
     6601 cgtcggccct tttcaggaaa actgttatat catcggcgat cccgcaacgc gcgaaggcgc
     6661 cgtcattgat ccgggcgatg aagcgccacg tatcatcaag gctatggaaa atacaggcct
     6721 tcgttttaag tatatcatca atactcatgc tcatatagat catgtcggcg ccgtacaaag
     6781 tgtcaaagat catttcgcta ttccgtttta tctgcaccgc gacgatgagg tcatactcag
     6841 gcatatgccc caatcggcgg ctatgttcgg tctgaaagtc ggacgcgtac ctgatataga
     6901 gttttatttt aatccaccgg cggatatgag gcttggtgat ttatcgttta aaatttttca
     6961 tgcacccggt cactctccgg gcagtgtttg tttttatttt gcgatgcaac acatacttat
     7021 cgccggggac gtactcttta acggttccat cgggcgaacg gatttgcccg gaggcgattt
     7081 tgacacgctg atcaactcga tccgccagaa tatttttaca cttcctgagg aaacgacggt
     7141 atatccagga catgggccgg aaacaaccgt gggttttgaa aaaaaattta atcctttttt
     7201 tgatcataac ggccttatgg cctaattttt aacaaagttt gagggaacaa tgttaagagt
     7261 acttctcatc gtattttatg tagggtttta ttcaacgtgg gcagcacaaa gcgatgtctt
     7321 atcaaagaca acagccgaca gtgtcaatta ttatctgctt catcccgtgt taaccaatca
     7381 ggcttacgaa gtttctaatt acaccttaac gcgcgatcgt gcgaagttcg tattaaaaaa
     7441 cggtttcatc tatacggcga agccgttttc cggaaatacg atcatggctc tgtttttggg
     7501 taccggtgaa tttgaactga cacctccggg cgacatcgaa caaaaaaacc tgactcgatt
     7561 tctgggtaca tcaacgctca gcaaagaatt tactaatcta ttttttgtct ttacggacag
     7621 tacggatcaa tggatttata gaaatgaacc ggcaacttta accgaggatg aattcaaaaa
     7681 cgcagcacgg ttttgtgagt ccgccgttaa atatttagcc gatccattcg gcgaatatgt
     7741 agatcagcat acagaactaa tgaatgacct cgtcaaccga cgtaagcagg ggatttattt
     7801 atccgttttt tccacgttat ctgctaacga tttaggatca tcaactacga ttttgtcgaa
     7861 atggcgtgat gaacctttga tttataaaat tgatcctacg gctaatgatg gcgaacatgt
     7921 gaccctcggt cgccgcattc gaaatcaagg gaaagggttt tatcaggaga tcgttagtaa
     7981 atttcctatg attccggata actcggttcc gtattttttg cgtacggaac gtaaaaatga
     8041 ttttaaaatc tccgattata acatgcgcat gacgattaac gaaaatttgt catcacaaac
     8101 atcggcgcaa ttaaccatgc gattgaacaa tccggttaac tggattcggt tgtttttata
     8161 ttatcgcttg cacgtagatt ccgtaaaaat caatgggcgt catgttcctt ttcatgctcc
     8221 cgtggctaaa gatgacggat tcgaacgttt tgaaagttcg ccaatatgga taaacacagc
     8281 ttccgttgcc gaacaaacag aaccgatacg cgtcgaaatt ttttatcatg gaaagttttt
     8341 agagcgtgaa ggagattatg tttatttcaa agcaaccgat gattggtatc cggccggatt
     8401 taccgggcgt catgatcgtt ttagttttga tattacgtac acatatcctt ccacttacat
     8461 gttagtctcg catggcacgg aacgagaaat tgaaaaaaat aaaaaaacga ttactgtaca
     8521 atggataacc cgccaaccga cacgccatgt gacatttaat atgggcgttt acaaggatat
     8581 tcgttttaaa gacgaacgca ttcctgatat tcatatggtg tttcgttatg accgcaacgg
     8641 taaagatgtt aaaaaacaag tcatggcgga tgtagccaat agttacatgt tttttcaaga
     8701 gaaatttggt tcattaccta taggtaaact tgtagccgca gaatattctt attcacatag
     8761 cgaagcctat gacgggttgg tcaatatttc agcatcagtt ttcaactcca atgacgatga
     8821 cggcgttgac gagtggattc gtgcgcacga agtagcgcat cagtggtggg gtattggcgt
     8881 tgatttcgat tcgtaccacg atcaatggtt gagcgaggcc ttcgccgaat acagcggtat
     8941 gttgtacgta caacaaagtt tatcggacaa taaagaattt tttcggtttt taaatatcac
     9001 caaggaacgt atttttaaaa accgtaaggg tatattcgtt gacggacaag aggccggtcc
     9061 catatcgctc ggataccgga cgtcttcaag ttctacgcgc ggcgattatg gtttagtcat
     9121 ctatagcaaa ggtatgtggg tattgcatat gctacgcaac atgatgcttg atctcaatac
     9181 gatgaacgag gatcgttttt ttgcgatgat gaaagattat tttgagacgt attacggaaa
     9241 aaaagccact accgacgact ttaaaagaat tgtcgaaaag catgttggga cggacatggc
     9301 atggtttttt gatcaatgga tccatgggac agatttacca aaatatgaat ttgcatacac
     9361 tacaaaacaa acagagaacg ggaaattcaa agtacgctgt cgtattcgtc aggaaaatgc
     9421 aggtgaaaat tttcaaatgt ttattcccat aactattgat tttggcgata accggaaagc
     9481 acggatgcgt ttattggtaa agggtccgca atctgaaata gatctacctc tgttgccttt
     9541 ggaacccaaa aaaatcatct ttaatgattt tgaatccgta ttgtgtgaag taaattattc
     9601 caactggtaa tgtaatagga ttaataatta tgagtgaaac gatagcgccg gtattcaaac
     9661 cggcagaccc aagcccggta aaacgcttag tatccaagag agatatcggt atatccgcgt
     9721 ttgaattgga aaacgggaac gtcatgcttg aagcgacttt tttagatccg tatcatttat
     9781 tgaggctcaa tattcagatt gatccgcaca ctcggtatat cgtgagttcc aagatggaaa
     9841 tggctaatca tccgcattca gcatgccccg gggtaacaga gaaagccaaa cttttgatcg
     9901 gtctgcaagt cacacgaggc attaccaaag agattagccg gcgcatcggc ggaagcgacg
     9961 gttgtgtaca tttgcgcgaa ctggcgctgg aaacgataaa ttttgcagcg acagttatgg
    10021 tgggctatga tgaaggattt gggcttatga gccgcgaatt caatatactg gacgaacata
    10081 agcgctacga tatctctaaa agtattttac gaaatacctg tcatatttat aaacaacctc
    10141 ggacgcaaga aggagaaacg gtatgaaggt ggataccaaa ataggagcgt accatttaca
    10201 tccggtcgaa acgggtcgtt ttgcactaga cggcggtgcg atgtttggtg tagtgcctaa
    10261 aaatctgtgg aataaaacca atccggccga tgaacaaaat cgcattacgc tggcaatgcg
    10321 ttgtttattg ctcgtttcgg atgatggaaa aaaaatactg atagataacg gtgtcggtca
    10381 taaatataat gaaaaatttg cctccatgta tcgcattgat cattcggttt acacactgga
    10441 gcgatctttg caaaaatacg atctcacccc aaacgatatt acggatgtca ttctgacgca
    10501 cttacatttt gaccatgcgg gcggttcgac caaaatggat gaacaaagcc ggatcgtcgc
    10561 cgcttttccc aacgccaaat attatgttca acaaaaacat tacgaatggg gtgaaaaagc
    10621 atctgaaaaa gatcgtgcca gctttctgaa accggattac gagccgcttg tccaatcagg
    10681 tcagttgcaa ttgatcgaac aaaccgacgg cattttcccc aatattactt tttttatagc
    10741 caatgggcat tcgccgtatc aacaacttcc tattatccat gatgcgcagc atacggtctt
    10801 tttttgcggt gatttgattc cgacgacgac acatattgcc gtaccctacg tgatggcgta
    10861 tgataatcaa cctttggtaa ccatagcgga aaaaaaggac atattgactc gcgcattcga
    10921 aaaccggtgg atattatttt tcgagcatga tccggagacc gctatgtccg gtgtacgccg
    10981 cgacgaaaaa ggatttcgaa tgggcgatat agttgcggtt aaaggatgat atgatcaaag
    11041 ggaagaaagt tacagccata ttaccggcgg ccggcatcgg taaacgcatg ggcgtcggtt
    11101 ccaaaaagca atttttggaa gtccacggga agcctattct tgtacatacc ctcgaaaaat
    11161 ttcatcatgc gatctatgtg gatgaagtca tattagtact gcaaaatgag tggataccgt
    11221 ttgttcaaga agaaatcatc ggcccgtacg gctttagtcg tattcataca ttagtgcccg
    11281 gcggcacaga acgacaagac agtgtttacg aaggcctaaa agcggtcgag gagtcagata
    11341 ttgtgcttgt tcacgatgcc gtgcgtccgt gtgtacgtgc cgataaaata gatgagctta
    11401 tcgaaataac cgtcgaatat tcggccgcca tactggctgt aagaccaaaa gatacgatca
    11461 aacaacaaga cgcaaatggt tttgttgcgc gtactattga tcgcaatact ctgtggaatg
    11521 tacagacacc ccaagcattt caatattcgt tattacggag cgctttcgat aaagcgtatg
    11581 ctgaaaacta ctatggaacc gatgaagcta tgctgatcga acgcataggt catgccgtaa
    11641 aaatcgttga aggtcactat gataatataa aaatcacaac gccggaagat ctagcactgg
    11701 ccgaacggtt tttgaaacac taaaaatacg ttcttgcatg cgaccttcct tttcttgtac
    11761 attaacactc aatttttaat ctatatgttt ttttatgtga atcagggagt tcgataccat
    11821 gaatgaggag tacaaacaac acatggataa aggccgtgag gcttttgtcg ccaaagattt
    11881 tcgcaaatcg tttgagtatt acaatgcggc cttgaatctg gtcaaagatc tcaaagatca
    11941 cgatgatacc aaacagcatt attttttgac gcaggaatgc gtatttgatg cgcttgaaat
    12001 gatgcaggac tacaagcaag cgattcaacg tattgatgag gtgatcgccg aagaacaagc
    12061 caaaggcgat acgacaacga agcacatggc gcacttcatg caacgtaaag cttgcttgga
    12121 aatcaaaagc gggaataacc gtccgggtgt aaaattagcc ggcgaagcgt atgaattggg
    12181 atggaagacc aaagaacaaa aaaccaatta tgaaatcgcc gcacaaatct ggaatgagca
    12241 tcaacgcggt tccaaaatca cgacggaatg ggttgaatac tggcaaaaac attttaacta
    12301 ttgggttgcc gataaagaac tggcaatatg attttttaca tcaagccgcc tgaaatggcg
    12361 gcttttttgt tgccgaagcg ccatggagca gcaaagaaat tcttgcttat tctcgactgc
    12421 ttctctatat tcgcggcgtt attgcaatac catcatatac gtttaagata aggatcttgc
    12481 catggatttt aaactctctg aagaacagga aatgattcgc cagacggctc gcgattttgc
    12541 acaggaacat ttggctccgc atgctgccga acgtgatgaa aaacagtttt ttcctaaaga
    12601 tgtgatcaaa caaatgggcg aacttggctt catgggtatg atggtcaatg ataaatgggg
    12661 cggcgccggt ttggatacaa tttcgtacgt tcatgtgatt atggaattag ccaaagccga
    12721 cgcttcggcg gctgttatcg catcggtcaa caactcttta gtttgtttta atatcgagaa
    12781 atacggaacc gatgcgcaaa aagaaaagta tctgaaaccg cttgcaacgg gcggtgcact
    12841 tggtgcgtat tcgctctcgg aagccgtatc cggcagcgat gctgcaggtc tgatttgttt
    12901 tgcagaaaac aaaggtgatc actacatcgt caacggtact aagttatggg tgaccaacgg
    12961 ttatcattcc gattacgtgg tatgttttat acgtacggac aaatccaaca agaccaaagg
    13021 catcagcgcg ttcgtcgttg ataaatcatt tccgggtttt aaagtcggta aaaaagaaaa
    13081 taaactgggt attcgcgctt cggacacgac agaactcgta ttcgaaaact gcaaagtacc
    13141 caaagagaat cttctcggtc aggaaggcct cggtttcaaa atcgctatgg aaactttgga
    13201 tggcggtcgt atcggcattg ccgcacaagc gatcggcatt gcagaagcag cattggaaga
    13261 aactgtgaaa tataccaaag cccgcgaaca gtttgggcaa ccgatagcca aatttcaagc
    13321 caacgaattt aaaatcgccg atatggccat gcgtattgat gccgccaaat tattgttatt
    13381 ccgcgcatgc actctgaaag atcaaggtca gaagtttggt aaagaagcgg ccatggcgaa
    13441 gctttttgcc tctgaaaccg caatgtgggt tactacacaa gcggtacaaa tgcacggtgg
    13501 ttatggttac accaaagaat acccggtaga acgttttatg cgtgatgcca agatcaccga
    13561 aatctatgaa ggcacttccg aaattcagcg cgtcgtaatt gctcgtaacg ttttaggcta
    13621 atctcgcgcg gaatttaaga aagaatacgt catgcgcact tttgaaatga tagaagaaaa
    13681 ggaacacgaa caagtcgttt tctttagcga taaagcaagc ggccttcgtg ccattgtagc
    13741 gattcacgat acaactctag ggcctgctct tggaggctgc cgaatgtatc cttatgcgac
    13801 gacggaagag gcgctcaatg acgtacttcg gttatcccgc ggtatgtcct acaaagcggc
    13861 cgtagccgga ttgaatctag gtggaggcaa ggccgttatc atcggtgatc cgaaaaaaca
    13921 taagaacgaa attttgtttc gtacatttgg ccgttttatt caaggattag gaggtcgcta
    13981 tattacggcc gaagatgtgg gaacgagcgt aacggacatg gaatggattc gaatggaaac
    14041 ccggtatgta accggcatat cacgtgctca aggcggcagc ggtgatcctt cgccggtaac
    14101 ggctttagga acgtatcgcg gtctcaaagc ttgcgtagaa aaagtttttg gtacacaatc
    14161 gttgaaaggt ttacgaatcg ccgtacaagg cttaggtcat gtcggatatt atctggtgcg
    14221 acatctccat gatgaaggtg caaaactcgt cgtaaccgat gtggatggag aacgcgtcaa
    14281 acgtgtggtc agcgaattcg gtgctgagta tgttgattcg gataagatat acgatgcgga
    14341 cgttgatgtt ttttcaccgt gcgcgatggg cgcgattatc aatgatgaca ctatcgggcg
    14401 actgaaatgc aagattatcg ccggagcggc caataatcaa cttcaggatg aggcccgaca
    14461 cggtgctatg cttttggcga aaaaaataac ttatgccccg gattatgtga tcaatgccgg
    14521 agggcttatc aatgtttata acgaacttga aggatacaat caggaaaaag cattggcaca
    14581 agctaaaggc atttgcgata ttatgaaaaa gatattggag cttgccgaaa gagagaatat
    14641 ctcaacgcat gaagcttcca atcgcatcgc cgaagagcgc attcgtcaaa tcgggcatat
    14701 caaaagtcat tatgtgacac aaccggagtc ggttttaaaa cgtatagaac gataaataaa
    14761 tttataattt gatctttggc attattggaa cgtatgaacc cattgcttgc ggatgatgga
    14821 gcgaaacccg gtaaaaacac ggcgcctaaa aacagtcgcc gcgaagcccg ggaaacggtt
    14881 ttaaaagcat tgtatgctta tgaaatgact caagacaata tcaagcgagt acttgatgac
    14941 gtctgcgata attttacgga accggctacg caatttattc acactcttgt gcaacaaaca
    15001 atcgcgcacc aaatggaaat ggatgtgctg atccgagcaa gagctcaaaa ttgggaattt
    15061 gaacgaatag ccctcattga tcgtattttg atgcgcatgg ctctttgtga aatattgttt
    15121 tttgaagaca taccgccgaa ggtgtctatt aatgaaatga tcgaaatcag taaacgatat
    15181 agcacggata aaagttcaaa atttatcaat ggtattttgg actcgatcta tgaagatctt
    15241 aaaaatgtgg ggcgtattac caaaacaggc cgtggtatta tcgaatcatg agaaatagta
    15301 acaatcatat tataaagctt attcgtgaga ataagctttt tttattgcaa tgatttccgt
    15361 tgctaaaata ttacccatat ccgaggtaaa cggtcccgga aagcgttgcg tagtatgggt
    15421 tcagggttgc cctaaacgat gtccgggttg ctggaatccg gaattcttat catttgaaaa
    15481 atcaacaaat acggtttctg ccaaagaact tgtaacgatg atttcgaaag ccactcacgg
    15541 ttttcgagat attgaaggcg taacattcag tggcggtgaa ccgtttgctt atgcagaatg
    15601 tcttaccgaa gttgcgcaac tcttgcgtaa gcaaaatctg accatcatga gttattcagg
    15661 ttttactgtg gaggaaatac gaactaaggg taatttttat gtgtctttgt tagaagcttt
    15721 ggatatatta gtggacggag aatataaacg ggaattaaac gttaatcgtt tatggcgaag
    15781 ttcagccaat caacgcgtgc tttttttaac ggatcggtac attgcttatc aagctcacat
    15841 tgatgaagaa tatcgcgaat tggaatttac tctaaataca aatcaaatca caacaaccgg
    15901 ttttccggaa gtttcgatat taaaaaacct aacgaatcac agggaatgat cagttatggc
    15961 gtttaaaaat cttgtaatac tgacaatgat tgccggctct atttttgcac aacaaaaaaa
    16021 tataaaagaa ccgttcggca tggttatatc tttgcgcgcg aatattgaag ccaaaggtga
    16081 taaagccatc aaacaagggg atttactata taaaggtgat gtaattgatc ttgggcaagg
    16141 ggattattgt aaagtttttt gggcagacgg tgcagtgaaa gaatataatg gaaaatttaa
    16201 tgtgcctgat ctaaaacaat tgcgtaaaga aatattggcc atgttcgccg aacagtcggc
    16261 cacacaatcg tggtttttta aagctcagtc tcattattcc aaacaaattt atcgaagtga
    16321 t
//
LOCUS       DAOQGF010000075        16082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1668_length_16082_cov_5.826231, whole genome shotgun sequence.
ACCESSION   DAOQGF010000075 DAOQGF010000000
VERSION     DAOQGF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1668_length_16082_cov_5.826231"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /gene="fdhF"
                     /locus_tag="PK937_10695"
     CDS             <1..1964
                     /gene="fdhF"
                     /locus_tag="PK937_10695"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNH33083.1"
                     /translation="RDQKIVTVKPSMDGPVNKGHLCVKGRYSHGFVGSGDRITEPMIR
                     RDGAWQKVSWDEAYAFTASELKRISANYGASSVGVIGSARATNEDNYLTQKFTRVVLQ
                     TNNVDSCARVCHAPSAKALGNILGTGAATNAFDDIERATTIFICGANATENHPIVGAR
                     IKQAALRGAHLIVMDPRVIELAEYADIHLAVRPGANVALLNAMACTVIEEGLTDDEFI
                     LAHVSGYEKLREFLKDFTPEKIADQCGVDAATIRQAARLYATAKPAMCMHGLGMTEHT
                     QGTDSVSGLINLALITGNMGKPGTGVNPLRGQNNVQGSAHMGCEPTHLTGYGTVDEGR
                     TLFEATWKVSLPTDKGLNLMHMLDAAEKNQFKAMWIVGYDVYMTNPDTDFSRRAFENM
                     ELVVIQDMFMNETARLFGTVFLPCTSSFEKDGTFMNGERRVQRVRKAIEPCGQSKPDW
                     LIICELAHAMGVREGFEFESAEDVWNEIRRVWKAGYGITYPKLEKGGIQWPCPTEDHP
                     GTDILHRTTFPVGARAEVQMLPFSAPHYDARYPWTLITGRSLFHFNSGTMTGRSKNAQ
                     IKRPDYVEIAPTDAAQLGVQTGDTLRITSENGQAFVPAIVTDHVKHREVYATFQEPEN
                     LLNAVIGMERDDKTATPNYKVTQVRIEKV"
     gene            2048..2121
                     /locus_tag="PK937_10700"
     tRNA            2048..2121
                     /locus_tag="PK937_10700"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2082..2084,aa:Asp,seq:gtc)
     gene            complement(2142..>2888)
                     /locus_tag="PK937_10705"
     CDS             complement(2142..>2888)
                     /locus_tag="PK937_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33084.1"
                     /translation="WKFNNVPPAIDKEINPRGGRQISVSIGYYMDNLIDGFAFQPSSG
                     SYTTLYKHDAYLRNENTWTEFIRLPFGDHSLEARLHTGFIPTKVDSFYNFFGGSLVGL
                     KGYSYYSIEGRYLGLFNAVYRFPLIRRIDKKAGPLYFDKIFGGLTFGMGNAWSTRSTM
                     DRWKRDVGIELRTEMYSFYVYPTRLTFNAAYGIDRFTGYGPMITKYKSEDGSYYNERQ
                     KVKSGGSWRYYLTVLFGFTLFDEAPKMKNP"
     assembly_gap    2889..2988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2989..5192)
                     /locus_tag="PK937_10710"
     CDS             complement(<2989..5192)
                     /locus_tag="PK937_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33085.1"
                     /translation="MKKTFLVFEILFFVLILVRSGYAQPEAWSHGELQWFTIRSAHFD
                     VHYHDSPYTALDVSRKGPERTAREAAAIAESIYETITHLYDHHPERIHIIIRDTDDYS
                     NGGAYYYDNKIEIWASNLNFELRGTHHWLRNVITHEFTHMISIQAMMKLSRRFPGIYL
                     QYIGYEKERRQDVLRGFPNRIVSYPVAGVMLPVWFAEGVAQYQQKDLDYEFWDAHRDM
                     ILRDRVLGEQMLTLNQMGTFGKNSIGNESAYNSGYALVSYIAAHYGDSSLKKITHYSS
                     KLFYSFERAVKVATGDDIDTIYRQWKNLITEQYALHTTDIRAHARIGREIATEGTGNF
                     YPALSPNGQYLAMISNRGFDYLSQASLYIVDPSNSNIIHEIKKAEGPLSWSPDSRYIA
                     YAKNTDDNDYHSQYNDIYVYDMVRRKEFRITCGLRAFAPAFSPDGSSLAAIINGDGTN
                     NIIRIVGFAEDVLNEKSTFSKNMFVRDGMHWRRITHFDDGRQIYGLCYTDNGRTLIFD
                     TSTDDGRDIFSVDTSGNHWKPVRQTRKDERSPVVSQDGQTLYFSADETGIFNLYAMNL
                     HSGDIRQITNVTGGAFYPAVGQNNILYYTLYQNTGFRVYSIADTTGFDSKQTAYGLEN
                     PNILSKPNDKPYPGKWPSEHRIFTVIPPRTDTAALYKNTFLKFSFLPVLRLDYGTFKP
                     GVYFYSSDVLSKSSFFGGAMFNIPDMERDLFGIFEYSGFGPTLFLELYNITRS"
     gene            complement(5189..6379)
                     /locus_tag="PK937_10715"
     CDS             complement(5189..6379)
                     /locus_tag="PK937_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNH33086.1"
                     /translation="MTRKRIIFFSGKGGVGKTTIAAATALRCADRGTKTILLSTDPAH
                     SIGDLLGLPIHATPTPVAPNLDAVHIDPHAELDSNWGHIRDYFSNLIQTLGADGAVSG
                     ELAILPGMDNLFSLLRIRDFHDKSDYKVIIVDMAPTGESLRLLSLPQIVSLALKITRV
                     IEKYIVSPVIRPASRMSKSLRVVVAPEDVARSWEAILEKILAMRDLFEKESRTTTRLV
                     MQPESMIMNETQRALTYLNLFGMVVDQVIINRILPDETQKGYFRIWHETQKKYIHAIT
                     RDFTPIPIAHAPFFHHEIHGLNDLRTLGNALYNQADPASVFYNSRPVQFVNTKKEKLM
                     KLKAPFLDVQNTPVSLHRRGNEIMIEIGSFLRTHTIPDSMAALEPEGADYNNGELIIR
                     FIKK"
     gene            complement(6389..6910)
                     /locus_tag="PK937_10720"
     CDS             complement(6389..6910)
                     /locus_tag="PK937_10720"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH33087.1"
                     /translation="MKSIIKSLYTIILILSQFGIIGCSSVPKTVSHAKTVKGEMMLIG
                     NITREELFQEMPVFQKNYDVYNPAGSAIETLHRMSTPTDILIVLGTWCGDSKHHVPSF
                     LKTLDAAKNPNLTYRLLAVDRTKKDSLGVTTQYNIKRVPTMIVLRNGAEIGRMVEFPQ
                     KTVEEDLLDIVKP"
     gene            complement(6894..7703)
                     /gene="tatC"
                     /locus_tag="PK937_10725"
     CDS             complement(6894..7703)
                     /gene="tatC"
                     /locus_tag="PK937_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNH33088.1"
                     /translation="MADEEKRALPGSTGGDMTFLDHLGELRDRLIKIVLAVLLMMAIS
                     FVYSNTIIKWLLYPPMIIPDLKPPIQLMRPVITQVQGLMMVKIQVGLIAGIVLSIPVI
                     AYQLWRFISPALKKSEKRFAIPLIFFITFFFLLGATFAYLVVIPITLNFLLSMGNIDT
                     ELGIVVENMIDVDQYLSFVSGFMLISGATFEMPVIAFFLAKIGILSPQFMREYWRHAA
                     VACLLVSAVITPTTDMITMFVVAAPMVVLYEMSIWVAKFATRKKKSDEKHN"
     gene            complement(7710..8144)
                     /locus_tag="PK937_10730"
     CDS             complement(7710..8144)
                     /locus_tag="PK937_10730"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNH33089.1"
                     /translation="MKEIIRDIRFEIQHMDRSPASARRFGFILTTILLLIAGWLWYKE
                     RDVWMYWAAPGVLVGLISAIAPNLIYPLYTGATIFSIFIGYFVSRVILLIMFTLFFVP
                     VGLITRLFGRDLLKKQIDPKTNTYWTKKESPVKTPEHYERMF"
     gene            complement(8158..9978)
                     /locus_tag="PK937_10735"
     CDS             complement(8158..9978)
                     /locus_tag="PK937_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNH33090.1"
                     /translation="MNILGISCFYHDSAAALVRDGKIIAAAQEERFTRKKHDDNFPKH
                     AVEFCLKTAGIDMSQIDVVAFYEKPLVKFERLLRTYFAYAPIGFNSFIKAMPQWIRKK
                     LWMQELLMEELPGFKGKIIYPEHHESHAAAAFFPSPFQDAAFLTIDGVGEWTTTSFGT
                     GHGNKLQIDYEIHFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPKYVDLIYKH
                     LIDLKEDGSFRMNMDYFDFAAGLTMTNAKFDAIFGGPPRKPESRLGQREMDLAKSVQV
                     VTEEIMMRMAKHVRKITGQKNLCLAGGVALNCVANGKLLKERVFENIWIQPAAGDAGS
                     ALGAALFGWYQYANNPRNADNINDTQFASYLGPDFTDAEIEAYLKSHDIPYKKHDRSA
                     MVQSVAGILADEKVVGWFQNRMEFGPRALGSRSIIGDARSTKMQSVMNLKIKYRESFR
                     PFAPSVLREKMTDYFDVDYDSKYMLLVADVKDERRIAMTEEQKKLFGIDKLNVPRSDV
                     PAITHVDYSARIQTVHQNDHGVYYELIKAFYDKTGCPVIVNTSFNVRGEPIVCTPEDA
                     FRCFMRTEMDYLVLGNFVLDKKEQKPVQDNKDWMNEFELD"
     gene            complement(9988..11514)
                     /locus_tag="PK937_10740"
     CDS             complement(9988..11514)
                     /locus_tag="PK937_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNH33091.1"
                     /translation="MKNIIRTVIISGIFGFYALRAQTYADHSASVFIPIDHWMYSYIT
                     LLQDRGHLASLNPSVRPYTRKEVFGAVKKLNQTDLSSAEKKWIQYIREELSDVAEDVA
                     CVDTTLIRTSWEGRLSLRRQQNDTHGDYFVKPNLEISSDNFAIHLRARIDHALRRDTN
                     YQGRKPSIGGAYLPDAYALLQWKSVRLTVGHYSTNWGPFENKSLIVSANPYPYERLEL
                     SWTTRRISIRSFVAQLDQAYNAGRYFSAHRMDIHLPHGINLGITESVIHGNADKTQPI
                     NWKYLNPINIFAEAQLNGGGDANENIAFDAYVPVGRFVFKGQFLVDDLILDGPDKPAP
                     NRKTSPDRLGFLITGIINNPGLPSSQAQLDYERVSSYTYNIKSSRPWQSYTYNGLGLG
                     SPRNDRDAWILTWRYFGFERWLLSLESGFFRQGERNLSSHDFTDSTMTVGKFPRGLVE
                     KTWRLGIQADYFHAHNLWTTCKISTDNVRNRSNKKTPLRHYLNVFVSLNYQIDRYLNI
                     "
     gene            complement(11511..12578)
                     /locus_tag="PK937_10745"
     CDS             complement(11511..12578)
                     /locus_tag="PK937_10745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNH33092.1"
                     /translation="MSFIATFLAGLIIAVGVTPMLRKKLLARGVIDQPDARKVHTRAI
                     PRLGGVAIYLGFVVAVLTAYALKGAAFDEKITQFVGLLVAGTFIAVVGVFDDVYNVRA
                     IYKLFTQIAAAMILISFDISMGSLSNPFGGSIELGVWNYPVTIFWVIGVINAINLVDG
                     LDGLASGVSLIVTMTIFLIALYLEKTYVALIALGLAGSILGFLRHNFYPAKIFMGDTG
                     SMFIGLMISALGLISSQKSAVSFAILVPFIALGYPVIDTALAIFRRARAGKSIFTADR
                     EHIHHILLSYGYSHPKTVIFLYVICLFFATMAFLFAVYNKSGTFTVGVLFFVLIFIFL
                     FIRMITDFKKKTEEDQETDDL"
     gene            complement(12600..13400)
                     /locus_tag="PK937_10750"
     CDS             complement(12600..13400)
                     /locus_tag="PK937_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNH33093.1"
                     /translation="MIDTLHTIRVLGFPTTTLDRRSLLSEINRWITVRDASRHLMALN
                     PIKVIRSRNEADLVRHIMQADMVYPDAFGISWAMSVFSGNKFKPIPGCDLMLDLFAMA
                     AQSGYKIFLLGASETVVSEAVQYIATHHAGAQIAGFRHGYFSSEDEKQKTVQQIIDTR
                     PDMVFVAMGALIQENWIQAIQKACTHQNISIPLLMGVGGSFDAITGHVPRPPRWMLRL
                     HLEWLFRLLQQPFRAPRMLALPKFATLVLLKKWFGINTDVQTDDFKKK"
     gene            complement(13397..15121)
                     /locus_tag="PK937_10755"
     CDS             complement(13397..15121)
                     /locus_tag="PK937_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNH33094.1"
                     /translation="MVILGISTGHDAGACVMIDGKIISAINEERLNRKKMYWGFPTLS
                     IPECLRLAQIQPGAIQAVAIAGTSSTRAKPMEFGYKSVGGFRRIFAELSRTPLTGLLM
                     GTEIGTKLTRKVFESSFFRGTSEIDSALSAAGIHAPIHFIDHHLSHATSAYYTSGWND
                     CVTITLDASGDGWCSRVYDCENGTMKLINSIPAYHSPGFYYCYVTHLLGFIALRHEGK
                     VTGLAAFGKSDQTAAIFSERIAYDSNKFRFDNRGGWLQGEMAHLQKQLEGFSKEDIAA
                     GIQKHLEDMVTAYAKDVVRKTGRKRIALSGGVFSNVKLNQDVWKSTGVEEVYVFPNMG
                     DGGLATGAAFEVFRQKKGTCLPYRLHDVYLGTSYNDSEIETALRQSGFPYKRYDNVED
                     EIGRLLSEKKIVARFNGGMEYGPRALGNRSILYHCQDRTVNAWLNDQLNRTEFMPFAP
                     VLLKEDAHEYLTDYDPNRAYSAEHMTITYSVTQKCADTAPAITHVDQTARPQLVTEDL
                     NASYYRILKAYKQRTGHSILVNTSFNMHEEPIVRSPEEAIKAYEQSRLHALAIGNYIV
                     EVDWSTRP"
     gene            complement(15102..>16082)
                     /locus_tag="PK937_10760"
     CDS             complement(15102..>16082)
                     /locus_tag="PK937_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HNH33095.1"
                     /translation="HNPKGLGLFLSATAMAYRATDDKNYLKSLEHLTTLLRQNESQGY
                     SGSCWGYNFDWQSRVFFVPKYTPTIVNTSFVAQAFVDAFIATGREEFLVKARSACDFI
                     IRDLYHTREGDRLSFSYTPIDRLKVHNANILGAGLLARVYAHTKEKELYDYAKASAEY
                     VLHYQRSDGSWYYAEEAIQHWIDSFHTGFVLDSLDWYVRGTGDQTPVNAIKTGFRYYI
                     ENFFLEDGTPKYYHDRIYPIDAHSPAQAFVTLTRLGGLDQRASNIRDHVRDWTLLHMR
                     SPKGYFYFQKHPNYINKIPYMRWVQAWSYYGLAHLLFYDTFKEIHGYSRY"
BASE COUNT         4394 a         3632 c         3790 g         4166 t
ORIGIN      
        1 cccgcgatca aaaaattgtc accgtaaaac cgtctatgga cgggccggtc aataaaggcc
       61 atctttgcgt caaagggcgt tattcgcacg gttttgtcgg atcgggtgat cgtatcacag
      121 agcctatgat ccgacgtgac ggggcttggc aaaaagtatc atgggatgaa gcgtatgcct
      181 ttaccgcatc cgaactcaag cgtatcagcg ccaactacgg agcgagcagt gtcggcgtta
      241 tcggttcggc acgggctact aatgaagaca attatttgac acaaaaattt acccgcgtgg
      301 tattgcagac caataacgta gattcgtgtg cacgtgtttg ccacgcaccg agcgccaaag
      361 ccttgggtaa tatcctcggg accggcgcgg cgaccaatgc ctttgatgat atcgaacgcg
      421 ctacgaccat ttttatttgt ggcgccaatg cgactgaaaa tcatccgatc gtgggcgccc
      481 gcatcaaaca agccgcgttg cgcggtgctc accttatcgt catggatccg cgcgttatcg
      541 aattggccga atatgccgac attcacttag cggttcgtcc cggtgccaat gtggctctgc
      601 tcaatgcgat ggcttgcacc gtgatcgaag agggcttgac ggatgacgaa tttatcctcg
      661 cccatgtgag cggttacgaa aaattgcgcg aatttctcaa agattttaca cccgaaaaaa
      721 tagcggatca atgcggcgtg gatgcggcaa cgattcgcca ggcggcgcgt ctttatgcga
      781 ccgccaaacc cgcgatgtgt atgcacggtc tgggcatgac tgaacacacg cagggtacgg
      841 actccgtgag cgggctgatc aatcttgcgc ttataaccgg taatatgggc aaacccggaa
      901 caggcgttaa tcctttacgt ggacaaaata acgtacaagg ctcggcgcac atgggttgtg
      961 aaccgaccca tcttacggga tatggcacgg tggatgaagg gcgcacttta ttcgaagcga
     1021 catggaaggt gagtttaccg acggacaaag gtctcaatct catgcacatg ttggatgccg
     1081 cagaaaaaaa tcaattcaaa gccatgtgga tcgtgggcta tgacgtatat atgactaatc
     1141 ccgatacgga tttttcacgt cgtgcttttg agaatatgga actcgtcgtg attcaggaca
     1201 tgtttatgaa tgaaacggcg cggttgttcg gtacggtgtt tttaccgtgt acctcgtcgt
     1261 tcgaaaagga cggaaccttc atgaatggag aacgccgggt gcagcgcgtg cgtaaggcta
     1321 tcgaaccgtg cggccaaagc aaaccggatt ggttaatcat ttgtgaatta gctcatgcga
     1381 tgggcgtacg tgaaggtttt gaatttgaat ccgccgaaga tgtatggaat gaaatcagac
     1441 gtgtatggaa agcaggctac gggattacct atcctaaatt agaaaaaggc ggtatccaat
     1501 ggccgtgtcc taccgaagat catccgggca cggatatttt acatcgtacg acgttcccgg
     1561 ttggcgcccg tgcggaagtg cagatgctac ctttttctgc gccgcattac gatgcgcggt
     1621 acccgtggac gcttattacc ggacgcagtt tgttccattt caattcaggt acgatgaccg
     1681 ggcgttctaa aaatgcgcag atcaaacggc cggattatgt ggagatagca ccgacggatg
     1741 cggcccagtt gggcgtgcaa accggagata cactccgtat taccagtgaa aacgggcaag
     1801 ccttcgtacc ggcgatcgtt acggatcatg taaaacatcg cgaagtgtat gcgacgtttc
     1861 aggaaccgga gaatttgctg aatgccgtta tcgggatgga acgtgatgac aagacggcga
     1921 cgcctaatta taaggtgacg caggtgcgta tcgaaaaagt atgatgtgcg agcgataaat
     1981 tttaccgaaa atcagaaaaa aaacttgctt ttcggatcgg attatactat catttcgccg
     2041 ctttaacggg cacgtagttc aattggttag agcaccgccc tgtcacggcg gaagttgcga
     2101 gttcgagcct cgtcgtgccc gcagtcaaat ccctgaaaaa ctcagggatt tttcatttta
     2161 ggggcctcat caaaaagtgt aaacccaaaa agtacggtca aataataacg ccacgaaccc
     2221 ccgcttttta ctttttggcg ctcattgtaa tatgatccgt cttcactctt gtatttggtt
     2281 atcatcgggc catatcctgt aaatcggtct atgccgtagg cggcattaaa agtaagccgg
     2341 gtagggtata cataaaatga atacatctct gtacgcaatt ctattcccac atcacgtttc
     2401 catcggtcca tagtagatcg tgtgctccag gcattgccca tgccaaatgt caaaccgccg
     2461 aaaattttat caaagtataa aggaccggct tttttgtcga tgcggcgaat gagagggaaa
     2521 cgatatacgg cattgaacaa gccgagataa cgtccttcga tgctgtagta agaataacct
     2581 ttcaatccta ctaaacttcc gccgaaaaaa ttataaaaac tatctacttt ggtcggaata
     2641 aaacctgtat gcaagcgtgc ttccagagag tgatcgccaa agggcaaacg tataaattca
     2701 gtccatgtgt tttcatttcg caaataggca tcgtgtttat atagtgtcgt atagctgccg
     2761 ctacttggtt gaaaagcaaa tccatcaatg aggttgtcca tgtaatagcc gatggataca
     2821 cttatttggc ggccgccgcg aggattgatt tctttatcaa tggccggcgg aacattatta
     2881 aatttccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ccgcgtaata
     3001 ttatagagtt ccaaaaataa tgtcggaccg aagccgctat actcaaaaat accaaataaa
     3061 tcgcgctcca tatccgggat attaaacatg gccccgccga aaaaagagga ttttgacaat
     3121 acatcgctgg agtaaaaata cacgcccggt ttgaaagtac cgtagtccaa acgtaaaacg
     3181 ggtaggaatg aaaatttgag gaatgtattt ttatatagcg ccgcggtatc cgtccgggga
     3241 ggtatgacgg taaatatgcg atgttccgaa ggccacttgc cgggatatgg tttatcattt
     3301 ggtttggaca atatattcgg gttttcaagt ccgtaggcag tttgttttga atcaaagccg
     3361 gttgtgtccg caatcgaata cacacggaat ccggtgttct gatacaacgt gtaatacaag
     3421 atgttgttct ggccgacggc cggatagaat gcgccaccgg taacatttgt tatttgacgt
     3481 atatcgccgc tatgcaaatt catagcgtac agattaaata ttcctgtttc gtcagccgaa
     3541 aaatacaagg tttgcccgtc ttgactgacg acggggctgc gttcatcttt gcgtgtttgg
     3601 cgcaccggtt tccaatggtt tccggaagta tccacgctga aaatatcacg gccatcgtcg
     3661 gtcgaagtat caaaaatcaa cgtacgtccg ttatcggtgt agcataggcc gtaaatttgc
     3721 cgcccgtcat caaaatgagt tatacgccgc cagtgcatcc cgtcacgtac aaacatgttt
     3781 ttggaaaaag tacttttttc attaagtaca tcctcggcaa atcctacgat gcgaatgata
     3841 ttattggttc catcgccatt gatgatagca gcgagcgatg acccgtccgg tgaaaaagcc
     3901 ggagcaaatg cacgcagacc gcaggtgatt cgaaattctt tgcgccgaac catatcatac
     3961 acgtagatgt cattgtattg cgaatgatag tcgttatcgt cagtattttt tgcataggcg
     4021 atgtaacggc tatcgggtga ccaacttaac gggccttccg cttttttgat ttcgtgaatg
     4081 atattcgagt ttgacggatc cacgatatag agagacgcct gactgagata gtcaaaaccg
     4141 cgattggaaa tcatcgcaag atattggccg ttcggagaaa gtgccggata aaaatttcct
     4201 gttccttctg ttgcgatttc acggcctata cgtgcatgtg cgcgtatatc cgtcgtatga
     4261 agcgcgtatt gctctgtaat caggttcttc cattggcggt agatcgtatc aatatcatcg
     4321 ccggttgcaa cctttacggc gcgttcgaac gaataaaaaa gtttggatga ataatgagtg
     4381 atttttttaa gcgatgaatc gccgtaatgg gccgcgatat agctcactaa tgcataaccg
     4441 ctattatatg ccgattcgtt gccgatactg tttttaccga acgtgcccat ctgattaagc
     4501 gtcagcattt gttcgcctaa gacgcgatca cgcaagatca tatcgcggtg ggcatcccaa
     4561 aattcgtaat ccaaatcttt ttgctggtac tgcgccacgc cttcggcaaa ccacacgggc
     4621 aacataacgc cggcaacggg ataggaaacg atgcggttcg gaaaaccgcg cagtacgtcc
     4681 tggcggcgct ccttttcata tcctatgtac tgcaggtaga tgccgggaaa acggcgtgat
     4741 agcttcatca tcgcctgtat agaaatcatg tgggtaaatt cgtgtgtgat tacattgcgc
     4801 agccaatgat gcgtaccgcg taattcaaag tttaggttgg acgcccagat ttcgatttta
     4861 ttatcataat agtaggcgcc gccgttggag tagtcatccg tatcgcggat gatgatgtgt
     4921 atcctttcgg ggtgatggtc gtagagatgg gtgatcgttt cgtaaataga ttccgcgatg
     4981 gccgccgctt cgcgggcggt gcgttccggg ccttttcggg aaacgtcaag agccgtgtat
     5041 ggcgaatcat gataatgtac atcaaaatgt gccgaacgga tcgtaaacca ttgcaattcg
     5101 ccatgcgacc aagcttccgg ttgtgcataa ccggatcgaa ccaaaatcaa aacgaaaaaa
     5161 agaatttcga agactaaaaa tgtctttttc atttttttat aaaccgaata ataagttcac
     5221 cgttattgta atccgcacct tcgggctcca acgcggccat ggaatcggga atagtatggg
     5281 tgcgcaaaaa ggatcctatt tcgatcatga tttcattgcc tctgcgatga agggaaaccg
     5341 gcgtattttg tacatccaaa aagggcgctt tgagtttcat taacttttcc tttttggtat
     5401 tcacaaattg tacggggcgt gaattgtaaa acaccgatgc gggatcggct tggttgtaca
     5461 gcgcattccc tagagtacgt aaatcgttta gtccgtggat ctcgtggtga aaaaaaggcg
     5521 catgggcgat cggtatcggc gtgaaatcac gggttatcgc atgaatatac tttttttgcg
     5581 tctcatgcca aatgcgaaaa tagccttttt gcgtttcgtc cggtagaata cgattgataa
     5641 tcacctgatc cacgaccatg ccaaataaat tgaggtaggt caacgcgcgc tgcgtttcat
     5701 tcatgatcat agattcgggt tgcatcacca gacgagtcgt agtgcgtgat tctttttcaa
     5761 aaagatcacg cattgccaaa attttttcaa gtatggcttc ccaggaacgc gccacgtctt
     5821 ccggtgcgac gacgacgcgc aacgatttag acatacgcga tgctgggcgt atcacgggag
     5881 aaacgatata tttttcgatc acgcgtgtta tttttaaagc cagtgatacg atttgcggca
     5941 aactcaagag ccgtaaactt tcgccggtcg gcgccatatc tacgatgatg actttataat
     6001 cggacttatc gtgaaaatcc cggattcgca aaagactgaa aagattatcc attcccggaa
     6061 gaatggccag ttcaccggag acggctccgt cagcgccaag cgtctgaata agattggaaa
     6121 aatagtcgcg gatatgcccc caattgctgt caagttcggc atggggatca atatgaaccg
     6181 catcgagatt gggtgcaacc ggagtgggtg tagcatgaat cggcaggcct aataaatcac
     6241 cgatactgtg agcgggatcc gtactcagaa gtatcgtttt agtaccgcga tccgcacatc
     6301 gcaacgccgt agcagcagcg atcgtagttt tacctacgcc ccctttaccc gaaaagaaaa
     6361 tgatacgttt acgtgtcaaa cgattccctt acggcttaac tatatctaat aaatcttctt
     6421 cgacggtttt ttgtggaaat tcaaccatgc gaccgatttc cgcgccgttt cgcaaaacga
     6481 tcatggtcgg tacgcgtttg atgttgtatt gggtcgttac acctagagaa tctttttttg
     6541 tccgatccac ggctaataag cggtaagtca agtttggatt tttggcggcg tccagtgttt
     6601 tgaggaatga gggtacatga tgtttggaat cgccgcacca cgttcccaat acaatcagta
     6661 tatcggtagg tgtgctcatg cggtgaagcg tttctattgc acttcctgcc ggattgtaga
     6721 cgtcgtagtt tttttggaaa accggcattt cctggaacaa ttcttcgcgc gtaatattgc
     6781 cgatgagcat catttcacct tttaccgttt tggcatggga aacggtttta ggaacggaac
     6841 tgcaaccgat gattccaaat tgtgataaaa tcagaatgat cgtatacaga gacttaatta
     6901 tgcttttcat cggatttctt ttttcgtgtg gcaaacttgg caacccagat gctcatttca
     6961 taaagtacca ccatcggcgc ggcaacgaca aacatggtga tcatatcggt cgtcggagtg
     7021 atgaccgcgg atacaagaag gcacgcaaca gcagcgtggc gccagtactc gcgcataaat
     7081 tggggtgaca gaatgccgat tttggcaaga aaaaaagcaa tcacgggcat ttcaaacgtc
     7141 gctccgctga taagcataaa tccggataca aaactcaagt actgatcaac atcaatcatg
     7201 ttttcaacca caataccgag ttcggtatca atatttccca tactcagaag gaaattaagc
     7261 gtgatcggta tgacgacaag gtaggcaaac gtcgccccga gcaggaaaaa aaacgtgata
     7321 aaaaaaataa gcggaatggc aaaacgtttt tctgattttt tgagtgccgg agagatgaat
     7381 cgccataatt gatacgcaat gacaggaatg ctcaatacga tacctgcgat aaggcctact
     7441 tgtattttga ccatcatcag accttgcacc tgcgtgatca cgggccgcat caactggatc
     7501 ggcggtttaa gatcggggat gatcatcggt ggataaagca accacttgat gatcgtatta
     7561 ctataaacaa acgatatcgc catcatcagc aaaacggcaa gcacgatttt tatcaagcga
     7621 tcccgcaatt caccgagatg atccagaaaa gtcatatcgc cgccggtaga accgggtagc
     7681 gcgcgttttt cttcgtcagc cataaaaatc taaaacattc tttcgtagtg ttcgggtgtt
     7741 tttaccgggg attctttttt ggtccaatag gtgtttgttt taggatcaat ttgttttttt
     7801 agcaaatctc ttccaaacaa acgcgtgatc aggcctaccg gaacaaagaa caatgtgaac
     7861 atgataagta gtatgacccg tgaaacaaaa tatccgataa aaattgaaaa aatcgtagcg
     7921 ccggtataca gaggatatat caggttaggt gcgatcgcgg atatcaatcc gactaaaact
     7981 ccgggtgccg cccaatacat ccaaacgtca cgctctttat accaaagcca tcctgcgatg
     8041 agtaataaaa tcgttgttaa aataaatccg aaacgccgag ccgaagcagg gcttcgatcc
     8101 atgtgctgaa tttcaaatcg tatatcgcga ataatttctt tcatagatcg tccttaatta
     8161 atcgagttca aattcattca tccagtcttt gttgtcctgg acgggctttt gttctttttt
     8221 atctaaaacg aaatttccca gaacgagata atccatctcc gtacgcataa aacaccggaa
     8281 cgcgtcttct ggcgtacata caatgggttc cccgcgcaca ttaaaggacg tattaacgat
     8341 caccgggcac cctgttttat cataaaaggc tttgatcagt tcgtaataca cgccatgatc
     8401 attttgatga acggtctgaa tgcgtgcaga ataatctacg tgggtgatcg ccggaacatc
     8461 cgaacgaggc acattaagtt tgtctatacc aaaaagcttt ttctgttctt cggtcatggc
     8521 gatgcgccgt tcatctttga catcggctac gagcagcatg tacttgctgt cgtaatctac
     8581 gtcaaaataa tccgtcattt tttcacgcaa aacgctcggc gcaaacgggc ggaatgattc
     8641 gcggtatttg attttcaggt tcattacgga ttgcattttc gtagaacggg cgtcgccgat
     8701 gatactgcgt gaacccaatg cacgcgggcc gaattccata cggttttgaa accaacccac
     8761 gaccttttcg tcggctagga tgccggcgac ggattgtacc atagcagagc gatcgtgttt
     8821 tttgtaaggg atatcgtggg attttaaata ggcttcaatt tcggcgtccg taaaatcagg
     8881 gccgaggtac gaagcaaact gtgtatcatt tatattatcg gcgttgcgcg gattgttggc
     8941 atattgatac cagccgaaaa gtgcggcacc caatgcgctg cctgcatcgc cagcggccgg
     9001 ttgtatccat atattttcaa aaacgcgttc tttgagcagt ttaccgttgg ccacgcaatt
     9061 gagcgccacc ccgccggcca aacagagatt tttttgaccg gtgattttcc gtacatgttt
     9121 ggccatacgc atcattattt cttcggtgac cacttgcacg gacttggcta gatccatttc
     9181 acgctgtccc aggcggcttt ccggtttacg cggcggtccc ccgaagatcg cgtcaaattt
     9241 agcattggtc attgtgaggc ctgcggcgaa atcaaaataa tccatattca tccgaaatga
     9301 cccgtcttct ttgaggtcaa tcaggtgttt gtagatcaga tcgacgtatt taggttcgcc
     9361 gtacggagca aggcccatga ctttgtattc gcctgaatta actttaaatc ccgtataata
     9421 cgtaaatgcc gaatacagaa gaccgagcga atgggggaag tgaatctcat agtctatttg
     9481 taatttattg ccgtgcccgg ttccaaaact ggtcgtagtc cattcaccga caccatcgat
     9541 tgtcaaaaag gcagcgtctt gaaacggaga cgggaaaaag gcggctgcag cgtgcgattc
     9601 gtgatgttcc ggataaatga tttttccttt aaagcccggt aactcctcca tcagaagctc
     9661 ctgcatccag agtttttttc tgatccactg cggcatggcc ttgataaacg aattgaatcc
     9721 tatcggcgca taagcaaaat aagtacgaag caatcgttca aacttaacga gcggtttttc
     9781 ataaaacgcg accacgtcaa tttgcgacat atcaataccg gctgttttta gacaaaattc
     9841 cacggcgtgt tttggaaaat tatcgtcgtg tttttttcgt gtaaaacgtt cttcttgtgc
     9901 agcggcgatg atctttccgt cacgcaccaa agccgcagcg gaatcatgat aaaaacaaga
     9961 tatgccgaga atgttcattg cttagtccta tatgttaaga tatcgatcta tctgataatt
    10021 aagggaaaca aatacattca agtaatgccg cagaggagtt tttttgttag agcggttgcg
    10081 gacattgtca gttgatattt tacaagtagt ccaaagatta tgtgcgtgaa aataatcggc
    10141 ctgaatacct aagcgccatg ttttttcgac taaacctctg ggaaatttgc ccacagtcat
    10201 ggtcgaatcg gtaaaatcat gactgctcaa attgcgctcg ccctggcgaa aaaaaccgct
    10261 ttccaaagac aatagccaac gctcaaaacc aaaatagcgc cacgtcagta tccatgcatc
    10321 gcgatcattg cgcgggctgc caaggcctaa tccattatag gtgtaggact gccatgggcg
    10381 cgacgattta atgttgtagg tgtaagagct gacacgttcg taatcaagct gggcttgaga
    10441 tgatgggaga ccgggattat taataatacc tgtgatcaga aatcccagcc gatccggtga
    10501 tgtttttcga ttcggcgccg gtttgtccgg gccgtcgagt atcaaatcgt ccacgaggaa
    10561 ctgaccttta aacacaaacc ggcctaccgg tacatacgca tcaaaagcga tattttcatt
    10621 cgcatcgccg ccgccgttta attgcgcttc ggcaaaaata ttgatcggat tgaggtattt
    10681 ccaatttatc ggttgcgtct tatcggcatt tccatgaata acagattcgg tgatacccag
    10741 attaataccg tgaggcaggt gaatatccat ccggtgcgca gaaaaatacc gccccgcatt
    10801 gtacgcttga tctaactgcg caacaaaact tcgtatagat atccgtcgtg tagtccaaga
    10861 tagttcgagc cgctcatagg gatacggatt ggcagaaaca atgaggcttt tattttcaaa
    10921 aggtccccag tttgtcgaat aatgaccgac ggttaatcga acactcttcc attgaagaag
    10981 cgcataggca tcgggaagat atgcgccacc gatacttggt ttacgcccct gataattggt
    11041 gtcccggcgc agtgcatgat ctatgcgcgc gcgcaaatgt atggcgaaat tatccgatga
    11101 gatttcgaga ttgggtttca caaaataatc gccatgcgta tcgttttgct ggcgacgcag
    11161 cgataaccgg ccttcccatg aagtgcgaat cagagtcgta tccacacagg cgacatcttc
    11221 tgcaacatcc gataattctt cccgaatata ttgaatccat tttttttcag cagaagaaag
    11281 gtcagtttga ttcaattttt ttacagcccc gaatacttct tttcgcgtat aaggccttac
    11341 tgaggggttg agcgatgcca aatgcccgcg atcctggaga agagtgatgt aagaatacat
    11401 ccagtgatct atgggaataa aaacggacgc ggaatgatcg gcataagttt gtgcgcgaag
    11461 tgcatagaaa ccgaagatac cggagatgat tacggtgcgg atgatatttt tcacagatcg
    11521 tctgtttcct gatcttcttc agttttcttt ttaaaatccg taatcatgcg gataaaaaga
    11581 aaaataaata tgagtacaaa aaacaaaacg ccgactgtga atgtgccgga tttattatat
    11641 acggcaaaga ggaatgccat cgtcgcaaag aaaaggcata tgacgtataa aaaaataacc
    11701 gttttgggat gagaatatcc gtacgataaa agaatatgat gtatatgctc acgatccgcc
    11761 gtaaaaatac ttttccctgc gcgtgctctc cgaaaaatag ccagtgccgt atcaatgacg
    11821 ggatagccca aagcaataaa aggaactaat atcgcaaagc taacggccga tttttgagag
    11881 ctgatcaaac ccaacgcaga aatcatcaaa ccgataaaca tgcttccggt atcgcccata
    11941 aaaattttag ccggataaaa attatgtctc aaaaaaccga gtatactccc ggctaatccc
    12001 agtgcgatca acgccacgta tgttttttca agatacagag cgatgaggaa aatggtcatg
    12061 gtaacgataa gtgaaacacc ggatgccaac ccatccagac cgtccacgag gttgatcgcg
    12121 ttaataacgc cgatgaccca aaaaatggta accggataat tccatacacc aagttcaata
    12181 gatccgccga acgggttgga taaactgccc atggaaatgt caaatgatat caaaatcata
    12241 gccgccgcga tttgcgtaaa aagtttgtaa atcgcacgaa cattatacac atcatcaaaa
    12301 acaccaacga cggcgataaa tgttcctgca acgagcaggc ctacgaattg agtgattttt
    12361 tcatcaaaag cagcgccttt aagagcatag gccgttaata cagcgactac aaatcctaaa
    12421 tagattgcaa ccccgccaag gcgcggaatc gctctcgtgt gaactttgcg tgcatccggt
    12481 tggtcaataa cgccgcgtgc caaaagtttt ttgcgaagca tcggtgtaac accgacggcg
    12541 attataagtc cggcgagaaa tgtagctata aaagacaaag tgaattcctg aaatgaattt
    12601 tatttttttt tgaaatcgtc ggtctgtaca tccgtattga taccaaacca ttttttcaaa
    12661 agtacaaggg tggcaaattt gggtagcgcc aacatacgcg gagcacgaaa cggctgttgt
    12721 aataatcgga aaagccactc caaatgcagg cgtaacatcc atcgcggagg acggggaaca
    12781 tgccctgtga tcgcgtcaaa actgccgcct acacccatga gcaaaggtat ggagatattt
    12841 tgatgagtac aagctttctg tatcgcctgt atccagtttt cctgtatcag tgcacccatc
    12901 gctacaaata ccatgtcagg gcgggtatca atgatttgct gaactgtttt ttgtttttca
    12961 tcttcacttg aaaaataacc gtggcgaaag ccggcaattt gcgcaccggc gtggtgcgtc
    13021 gcaatgtatt gtacggcttc ggatacaacg gtttctgaag cgcccagaag gaaaattttg
    13081 tatccggatt gagccgccat ggcaaaaagg tccaacatca gatcgcaacc gggaatcggt
    13141 ttaaatttat ttccggagaa aacactcatg gcccaactga taccgaacgc gtcagggtat
    13201 accatatccg cctgcataat gtgacgaacg aggtcggctt cgttgcgtga acggatcact
    13261 ttgatagggt tgagtgccat gagatggcgt gaagcatcac gcacggtgat ccagcggtta
    13321 atctccgaca gcagagatcg tcgatccaat gtggtcgtcg gaaaaccaag tacgcgtatg
    13381 gtatgcaagg tatcgatcat gggcgcgttg accagtccac ttcgacgata tagttaccga
    13441 tagccaaagc gtgcaaacgg ctttgttcgt aggctttgat cgcttcttcc ggggagcgca
    13501 cgatcggctc ctcatgcata ttgaaactgg tattgaccaa aatcgaatgc cctgtgcgct
    13561 gtttataagc ttttaggatg cgataatacg aggcgttaag atcttcggtg accaattgcg
    13621 gtctggccgt ttgatcaacg tgtgtgatgg ccggcgccgt gtcagcacat ttttgggtta
    13681 cgctgtacgt aatggtcata tgttccgcag agtaggcgcg gttgggatca taatcggtga
    13741 ggtattcatg cgcgtcttct ttaagtaaca ccggtgcaaa cggcatgaat tccgtacgat
    13801 tcagctgatc gttgagccac gcattgaccg tacgatcctg gcaatgatag agaatcgagc
    13861 gattgcccag cgcgcgcgga ccgtactcca taccgccgtt aaaacgcgcg acaatttttt
    13921 tctccgagag caatcggcct atttcatctt ctacgttgtc atagcgtttg taggggaatc
    13981 cggattgccg gagcgccgtt tctatttcac tgtcgttgta cgaagtgccc aaatatacat
    14041 cgtgcaaacg atagggtagg cacgttcctt ttttctgacg aaatacctca aaagccgcgc
    14101 ccgtagccaa tccgccgtct cccatgttag ggaaaacata aacttcttcg accccggtgg
    14161 atttccatac atcttggttg agcttgacat tagaaaatac accgccgctc aatgcgatgc
    14221 gcttacggcc ggttttacgt acaacgtctt ttgcgtaggc cgtaaccata tcttcaagat
    14281 gtttttggat tcctgcggca atgtcttctt tactgaatcc ttcgagctgt ttttgaagat
    14341 gcgccatttc accttggagc catccgccac ggttatcaaa acgaaatttg tttgaatcat
    14401 aggcgatacg ttcactaaag atggcggctg tttgatcaga cttgccgaat gcggcgagcc
    14461 cggtaacctt gccttcgtga cgcagtgcga taaaaccgag caaatgggtc acataacaat
    14521 aatagaaacc cggtgaatga taagcaggaa tggagttgat cagtttcatc gtaccgtttt
    14581 cacaatcgta cacgcgcgaa caccaaccgt caccgctggc atccagtgtg atggtcacgc
    14641 agtcattcca acccgacgta taataagccg atgtcgcatg ggataaatgg tgatcaataa
    14701 aatgaatggg tgcgtgaata cccgccgcgc tgagagccga atcaatttca gaagtgccgc
    14761 ggaaaaaaga agattcgaat acttttcgcg ttagtttagt gccgatttcc gttcccatca
    14821 aaagcccggt caggggtgta cggctgagtt cggcaaaaat acgacgaaaa ccaccgacgg
    14881 atttgtaacc aaactccata ggtttggcac gtgtggaact ggtaccggcg atcgctacgg
    14941 cttgaatagc acccggctgt atttgcgcca aacgcaaaca ttcggggatt gaaagcgtcg
    15001 gaaagcccca atacattttt tttcgattga gacgttcttc attgattgca gaaatgatct
    15061 tgccgtcaat cattacacaa gctccggcat catgaccggt gctaataccg agaataacca
    15121 tgaatctcct taaacgtatc gtaaaatagc agatgcgcta aaccgtaata tgaccaggct
    15181 tgaacccatc gcatataagg tatcttgttt atataattcg gatgcttttg aaagtaaaaa
    15241 tatcctttcg gtgaacgcat atggagcaac gtccaatcgc gaacatgatc ccgaatattt
    15301 gaagcccgct gatccagtcc gcctaaacgc gtaagcgtca caaaagcttg agccggactg
    15361 tgcgcgtcga tcggatatat tctgtcgtga taatatttag gtgtcccgtc ctctaaaaag
    15421 aaattctcta tgtaataacg aaaaccggtt tttatcgcat tcacaggtgt ttgatcgccc
    15481 gttccgcgga cataccaatc cagagaatcc agtacaaacc cggtatgaaa actatcgatc
    15541 caatgctgga tcgcttcttc ggcataatac caggagccgt cggaacgctg ataatgtaaa
    15601 acgtattcgg cgctggcttt ggcataatca tataattctt tctctttggt atgggcataa
    15661 acccttgcca gaagacccgc accgagaatg ttggcgttat gtactttgag ccgatctatc
    15721 ggtgtatagc taaacgaaag tcggtcaccc tcccgggtat gatacagatc gcgtatgatg
    15781 aaatcacacg ctgaacgcgc cttcactaaa aactcttcgc ggcctgtcgc aataaacgca
    15841 tcaacgaaag cctgagcgac aaacgacgta ttgacgatgg tcggcgtata tttgggtaca
    15901 aagaacacac ggctttgcca gtcaaaatta taaccccaac aactgcccga ataaccctga
    15961 ctttcatttt ggcggagcaa agtagtcaaa tgttccagcg atttcaaata atttttatca
    16021 tcggttgcgc ggtaagccat tgccgtggcc gacaagaaaa gtcccaggcc tttggggttg
    16081 tg
//
LOCUS       DAOQGF010000076        15943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1681_length_15943_cov_7.479985, whole genome shotgun sequence.
ACCESSION   DAOQGF010000076 DAOQGF010000000
VERSION     DAOQGF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_15943_cov_7.479985"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PK937_10765"
     CDS             complement(<1..157)
                     /locus_tag="PK937_10765"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNH33096.1"
                     /translation="MTTTSKTKKQRCWWCGDDPLYCEYHDKEWGVPVHDDQKIFEFLI
                     LEGFQAGL"
     gene            complement(181..753)
                     /locus_tag="PK937_10770"
     CDS             complement(181..753)
                     /locus_tag="PK937_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNH33097.1"
                     /translation="MREQQYDSESFVQIDFTKQRIALNTYDTCTFERCDFSKSDLAEV
                     RFLDCRFIGCNFTLCSVGNTVFGNAVFQECKMTGINFEKANTALFSASFIQCDLSSSL
                     FTKIKLRKTQFDNCILKSVDFTGCDLTDAKLSASDLAGAHFENTILEKADLRTAFNYS
                     IDPDINRIRKARFSQSGLHGLLEKYGIEVS"
     gene            complement(775..1359)
                     /gene="pnuC"
                     /locus_tag="PK937_10775"
     CDS             complement(775..1359)
                     /gene="pnuC"
                     /locus_tag="PK937_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNH33098.1"
                     /translation="MTLDWYEIFGFVTGVLCVALLIRQNIWNWPVAIGNAVFYIIVFY
                     NARLYADVVLQFFFIGINAYGWIVWLRRGPQGETLHVNSMDKVAWLWSLLAIAAGTTS
                     LGAWLHYNTDAALPYADAFGTATSLTAQFLMARKKWENWILWIAINIWYIGLYFVRDL
                     YLTQVLYVIYLGLAIAGLIEWRRAFRNPKQSLTN"
     gene            complement(1356..2105)
                     /locus_tag="PK937_10780"
     CDS             complement(1356..2105)
                     /locus_tag="PK937_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33099.1"
                     /translation="MAHYPVYFSDSTLALHRSDYHSLFGNDAPVVQETVRIQIGDTAL
                     TVAVTLSDSNALCLTPQDSFRLKLNPSILTSTPTPDFLKIVIMVHARGKETRFNGTLQ
                     LIERELLLQKHEMTQLKLIPGQVVFLAARKGRDTIFGGVRVIEVSGANRARIPRDEAQ
                     GALLQENDLLYLTGLGTPNSIPVSETPVLPERFLALDLKPGKSGTSWITEKDVWQAIL
                     KHKKIWVPKNARLTPAAADLGKSHRIFEFEA"
     gene            2099..3136
                     /locus_tag="PK937_10785"
     CDS             2099..3136
                     /locus_tag="PK937_10785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNH33100.1"
                     /translation="MPQQIEIQNCFDYVTQLTAFIQRRIPESMRKYQFNIPANERPKR
                     LDHFVTHAIENLSRSRAQQLVSENMVSVNGKFVAKSYMIRPGDRIEVILPLPEKVEAQ
                     AEDIPIRVVYEDDDILVVNKSAGMVVHPAYANYTGTLVNALLHHVQDLSGINGELRPG
                     IVHRIDKDTSGLLLVAKHDRAHRHLSKLFKDHNIEREYWALVWGKPKKKKDTIETLIG
                     RSTKDRKKFAVTKEGKNAITHYELITAYESVSLIRCRLETGRTHQIRVHMNYIGHPIL
                     GDKTYGGDNPNLAGGEKKKKQHAENILALIPRQALHAKTLGFIHPTTKKMMRFDSELP
                     DDFNAVLQLIK"
     gene            3204..6281
                     /locus_tag="PK937_10790"
     CDS             3204..6281
                     /locus_tag="PK937_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33101.1"
                     /translation="MRYIRVYLFVMIFAGGLRSYLSAQEISLSGDLQPFSVTAPGAIS
                     DEFSYAVSATGLSGSLLIHAPHGFEISTTSGGVFGSNITLPETGGEIELTTIYVRFAP
                     SVTGSYDSVIVHESGVTQTLAVTGLCAFSEPTLPPSDVVFSGIDSSAMTITWQGGDGD
                     YHLVLVRADALIELAPNDGQSITANSTFGSGTDIGGNTYAVFAGSGTSVSINGLSRGV
                     NYYFSVFTYNGTGNDADYLTDAAVGEQTTLSRPPERIQMIRDTTFAEDSGTRTIIADL
                     HDIFSDPENNLLTFSAGTLVSGADAWVTDNTLEFFSSEDSSGLFEVIVEAQDNSGNFV
                     YDTLLVTVTQQNDAPKLATPIGPKNLQEDFGKQLLAYLPLVFHEADGEAMTFSIHTPS
                     PYAALMISGDSLYALSQNNLNGQSQVIIEASDPTPLSNRDTFQLQIIPINDPPARTSV
                     LRDTSFAEDFSYRPLVYLPDYYSDPDDGVINFSSFVVNTGVEGYILNDSLYVEGNEDF
                     FGSAALEIRASDGNTEIRDTIFFTITPVNDAPSRIFIARDTSVQEDAARTAILYLPMI
                     FLEPDLEPVSYGVALSNIKSVAAISGDTLYVTPLKDSTGSVEVYLSATDPTDLSAKDT
                     LVLQIVPVNDRPVRLATISDTTLPQNFGKVLIKKLSTVFYDADHTALSFSFVKFADGV
                     DPLISNDSLYLRSTVDFFGNVSVRVIASDGEFTVSDTFRVVVQDNTAPVAFLAALSSP
                     LLQRIRFALGSNETIASAALTANGQVIDIESTNGVYFGDYSIESVGVIPVVGEVFDLA
                     GNKDTIRRSYTVTSLDKKTTYDNFVFEGNQRGYLVLDQAETGNVPTLLRPLSNAVDVL
                     NTASSAEVTVHVRYQDHLSALRAADENFDEGRIGVYAYENHQWQRLSGEGRFGAVETR
                     IHKNVRIAVFYDPEYIMQPKTFALDQNYPNPFNPSTTIRFAISQPGHTTLKVYNMLGQ
                     EVRTLFSEYKPAGNFETIWDGKNEKGLAVASGVYLYRLSSGSFTQTRRMVLVK"
     gene            6278..6841
                     /locus_tag="PK937_10795"
     CDS             6278..6841
                     /locus_tag="PK937_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33102.1"
                     /translation="MKTLSIRYLILLVSVFWITGCPSKDETAIEKAQQLFEDGDYEDA
                     LSHYESLIATEGSTARVGAGWSAMRLNDLNTANVYFSEAALDSIPDGYAGWSVTLWGS
                     GSLLQSLDKTAFVLRKKPEFKLSLDRSVTHKDLIWVQASCYYQLGNYPAAVNRIQALD
                     DTFAPNMEAANLAEVIALKLQALGVVH"
     gene            complement(6849..7832)
                     /locus_tag="PK937_10800"
     CDS             complement(6849..7832)
                     /locus_tag="PK937_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33103.1"
                     /translation="MLRFILKRGRAAIPMIFGLLTVTFFLIRLAPGDPTSLFIDPSID
                     PQAAAQLKENLGLNDPLPVQYGKWLGVIPPFEGLLQGELGISFSKQMPVVEVISDALI
                     NTMILTVAALIVDILVGVGLGVVSALRRGTIFDRMTTVVGLFFYSMPHFWLALVLIMI
                     FSLYLNWFPASQMHSIGYEVWPFWTRVQDLLAHMVLPVFVLGIASAASTIRYVRSSML
                     EVLQQDYIRTARAKGLPESLVIWKHGLRNALLPLITIIGLSFPFLLAGAVVTEVIFAW
                     PGMGRVIIDAIFARDYPLIIANTMMAGILVILGNLLADILYAVADPRARAE"
     gene            complement(7837..9522)
                     /locus_tag="PK937_10805"
     CDS             complement(7837..9522)
                     /locus_tag="PK937_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNH33104.1"
                     /translation="MSLILFYKSMRRWLIILVIIHLMGCVPHTPSDGGITTIALSSDP
                     ETLNPLFATGTNSSAVIYFLYPDLVEYDFNETTGRDTILPSLAKSWTISDDHSTLTYH
                     LHTHAHWHDGKKITAQDFAYTYRLYFDPQIGSARRTRIDIVQTRDDGSIDWENVIRTP
                     DDSTIVFYFKKNTPRHRMTEWAQQTPVPKHVLDTIPSSRLRTSWFNQKPVSGKHFTLE
                     RWERKQNLILKRNSEWTIPGKAHLDQIVFRIIPDMTTRLIELQSGRVDVVEGINPSDA
                     AELCKQSRFRIERQSGRRVEFVGWNHIDPSSYISSGTIKPHKLFSDQQVRKALSYAIH
                     RAEITQSWCGEYGQTASGPVSPVFLWAYNDQLPEMTYDPDSARIILQSAGWTDHDGDG
                     ILDKNGKPFQFTLTTNTGNPRREFIAQKIQSDLLRVGIKIEIQMLETAAFNAALRERK
                     LDAFITGYSVNMSLDLQPQFGSDLKKNTFNASGYQSAEADSLLNVIANAADRYSVADA
                     YRALQSVIYRDQPVTFLFWYDNLTVFNNRVRGTHADIYSPYHRFWEWYIEENP"
     gene            complement(9500..10327)
                     /locus_tag="PK937_10810"
     CDS             complement(9500..10327)
                     /locus_tag="PK937_10810"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNH33105.1"
                     /translation="MNSGLARIVVAIVALPFVIAAIIYGSWYFFALMGTILLIGISEM
                     LNLLRHKNSKPQTFSAFFWGLIFLGLTFFGKIEFLLSALFLFAIYTMTVELFRNTGSD
                     MMNMAATWMTVFYPSLLIGSMILLRNTGASESHSGAQLVFLVFLSIWACDTFAYYGGR
                     MMGKNKFFERVSPKKTWEGAITGFFGALIGVWLVRYIYLTLDIPFIMSLPQTLVIGFF
                     GGTFGQIGDLAESLLKRDAGIKDSGALLPGHGGILDRFDSMMFVAPLTYIYVTYFVL"
     gene            complement(10501..10704)
                     /locus_tag="PK937_10815"
     CDS             complement(10501..10704)
                     /locus_tag="PK937_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33106.1"
                     /translation="MAKKQTFVDKTAKVAAYAEKVKCEACKQESVASYAKFVESKVSE
                     KTGAWKFLERKVKLCGNCGTMIA"
     gene            complement(10733..10918)
                     /gene="rpmG"
                     /locus_tag="PK937_10820"
     CDS             complement(10733..10918)
                     /gene="rpmG"
                     /locus_tag="PK937_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011619264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNH33107.1"
                     /translation="MAKSKGNRIVITLECTSCKDSGLPGMSRYTTEKNKKNNTERLEV
                     KKYCNVERKHTLHKETR"
     gene            11162..11734
                     /locus_tag="PK937_10825"
     CDS             11162..11734
                     /locus_tag="PK937_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33108.1"
                     /translation="MIDFAEHAPLQSESGRLVLRDPYPEDAATMVMWRNESTVRAHQP
                     LRTVTVDTIEQDLLRSQSNVLPDYNRERFQWIIEKTEDRISIGWITLTIRSWEHEIGE
                     IGYSIATVFQGRGYGTEAIRLVLKKIFYEARLYRVEAKCSVENMASSRLLERLGFHRE
                     GIMREYFDIHGRRVDHYLYSLLRHEYFSKL"
     assembly_gap    11908..12007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12218..12291
                     /locus_tag="PK937_10830"
     tRNA            12218..12291
                     /locus_tag="PK937_10830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12252..12254,aa:Ala,seq:cgc)
     gene            12710..15019
                     /locus_tag="PK937_10835"
     CDS             12710..15019
                     /locus_tag="PK937_10835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH33109.1"
                     /translation="MMTFPARWTNHFRVAISQLLLFAGVVYFLFRWAEEDRALYLVLA
                     GITGTAFCVLALLHFTKTARIARRMSSVIIIAMTFCFVIVGYLFRGHIDHVIQEADLS
                     ANLDRLASYRANLNNRFLKHAEQGEFLRKRLTAEPFWRQPGILAEFLRHHTDDYFSVY
                     LLDDQGVSLLSEPVSTGYKESYPVRNLSNPAFVEPSFQPINDGNKVPAWHALKYFIPL
                     ETAEGQKRYVLLHIRTNAWLHEIKQSMPGVSVFLLHDNEIIATNEKHWNENIIREITN
                     EGYGRNSEEQIFFARYPLRSDNWSVVFLQTWNTAFPNVSYAESALVRVLIFSAVFGFL
                     TGVLLSWYLSRTILLPLSRLTNDVQIVVSGDQTHTLRESSSDPQDEIGALHRAIANIV
                     KSLKERTIQTESVQNAWSDVQSELEAQSDVVKKIQADTLRSSPLITRSLTINGRLVIA
                     QEHNGKFYDITEPQPGFAAFAIVDPAGKGAIGSFYAALMKGLLSAYWKRSDLLLQDPL
                     RWMNELETDFMKHRDTDTRTIALSVGIINTHDHRVRLINAGSEYPVLRKNDQTQSIAI
                     RGRALGMPQWLGSFETTTFVLALGETMFLHSHGISPDAMTEWPEKIHNTAMDSKQSDA
                     VILKIVRCESVKENFIFTPGSDVDFDAENKIQTMMARRGFESEKIKSMLSACNATITY
                     AARSTRLPELKNSIYVEAEACDDGIEVRITDQNNNSYWNMARIPEEFDQTESKGGRAW
                     NIGAIQKLVDEVIVEQSANKTTIVLRSIK"
     gene            complement(15023..>15943)
                     /locus_tag="PK937_10840"
     CDS             complement(15023..>15943)
                     /locus_tag="PK937_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33110.1"
                     /translation="ESDHKNHFFHKTIISFRSLLRKLVDLNEKTGERVTLEQLILTYL
                     HARLKAFLEDYEGPRDKHLESIKTRVSHCHDMHRQFAITDLVEEHIFENPGLLLTLSE
                     PDKYWPELINTFELNIMLEIYIEREYLISGHHDMTKRQMMETLQQLINGIQEISIKSL
                     SVVFLTAHLAAMQDHAKSKTVSLDEIIQTIAQLRKDAEMAAKQITVESRVKHDILTTL
                     TERLFRLETIQSAITLNNTTDIVDRLDEVIVGLRTRRDNLIGGKMDAKVLQDDLNTYL
                     HDSKVVGLIDEVERLYKNELAQQAKIVASK"
BASE COUNT         4336 a         3354 c         3763 g         4390 t
ORIGIN      
        1 tcaacccggc ctgaaatcct tcgagaatta aaaactcaaa tattttctga tcatcgtgta
       61 ctggtacgcc ccattcctta tcatgatatt cgcaatacaa agggtcgtcg ccgcaccacc
      121 agcatcgttg tttttttgtt ttcgaggttg ttgtcataac tttcggtcgg gttaaataat
      181 tcaactgact tctattccgt atttttcaag caaaccgtgt aaaccgcttt gtgaaaaacg
      241 cgcttttcgt atgcggttga tatccggatc aatcgaataa ttgaacgcgg tacgtaaatc
      301 cgctttttca agtatggtat tttcaaaatg cgctcccgcc agatcgctgg ccgacaattt
      361 tgcatcggtc agatcgcatc ccgtaaaatc aaccgatttt aatatgcagt tatcaaactg
      421 tgttttacgg agttttattt tggtgaataa ggatgaactg aggtcgcatt ggataaatga
      481 agcggaaaat aacgccgtat tggctttttc gaaattaatt cccgtcattt tacattcttg
      541 aaaaacagca ttaccgaaga cggtgttgcc cacgctgcac aaagtaaaat tacaaccgat
      601 gaaccggcaa tctaaaaaac gaacttccgc caaatccgat ttcgaaaaat cgcatcgttc
      661 aaatgtgcag gtatcgtagg tattcagtgc gatacgttgt ttggtgaaat caatttgtac
      721 aaaggattcc gagtcatact gttgttcgcg catcagatgg tacctgtgta acagttagtt
      781 ggtaagggac tgcttggggt tgcgaaaagc gcgtcgccat tcgatcaaac ccgcgatggc
      841 aaggccgaga taaatcacat aaaggacttg ggtaagatag aggtcccgaa caaaatataa
      901 accgatatac cagatattga tggcgatcca caaaatccaa ttttcccatt tttttcgggc
      961 catcaaaaat tgagccgtca aactggtggc cgttccgaag gcgtcggcat acggcagtgc
     1021 ggcgtcggta ttataatgga gccatgctcc gagggaagtc gtgcctgccg ctatcgccaa
     1081 cagagaccaa agccatgcga ccttatccat tgagttgacg tgcagcgtct caccttgagg
     1141 tccgcgtcgc agccagacta tccagccata cgcattgatg ccgatgaaaa aaaactgcaa
     1201 caccacatcg gcatacaaac gtgcattata aaatacgata atatagaaca cggcattgcc
     1261 gatagcgaca ggccagttcc agatattctg acggatcagg agggcgacgc ataggacacc
     1321 cgttacaaat ccgaaaattt cataccaatc aagcgtcatg cttcaaattc aaaaatgcga
     1381 tgtgatttgc cgagatctgc cgcggccgga gtaaggcggg catttttggg aacccagatt
     1441 tttttgtgtt tgagaatggc ttgccacaca tctttttcgg taatccacga tgtaccgctt
     1501 tttcctggtt tcagatccag cgctagaaaa cgttcgggta aaaccggtgt ttcggaaacg
     1561 ggaatagaat tcggcgtacc caacccggtg aggtaaagca gatcgttctc ctgtaaaaga
     1621 gcgccttgtg cttcatcacg tggtatcctg gcccgattgg cgccactgac ttcgatgaca
     1681 cgcacaccgc caaaaatcgt atcacgccct ttgcgcgcag caagaaaaac cacttggccg
     1741 ggaattagct taagctgtgt catttcgtgt ttttgcaaaa gaagttcgcg ttcgatcaat
     1801 tgcaaggtac cattgaagcg ggtctctttt ccgcgggcat gcaccataat aacgattttt
     1861 agaaaatccg gtgtcggggt tgatgtcaat atagagggat taagctttaa tcgaaaacta
     1921 tcttgtggcg taagacacag cgcattgctg tcggataatg tcacggcgac ggtaagggcc
     1981 gtatcaccga tctggatacg tacggtttcc tgtacgacag gtgcatcatt gccaaaaagg
     2041 gagtgataat ccgagcggtg taacgcaagg gtagagtcgg aaaaataaac cgggtaatgt
     2101 gccacagcaa attgaaattc agaattgttt tgactatgtt acacaattaa cggcattcat
     2161 tcaacggcga attcctgaaa gcatgcgaaa gtatcagttc aatataccgg ccaacgaacg
     2221 ccccaaacgt cttgatcact ttgtgaccca cgcgatcgaa aatctttcac gttcgcgcgc
     2281 gcaacagctg gtgtcggaaa acatggtcag tgtcaacggt aagtttgttg ccaaatcgta
     2341 tatgatacgc ccgggcgatc ggatcgaagt gatattacct ttgccggaaa aagtcgaagc
     2401 gcaagccgaa gatattccta ttcgtgtggt atatgaagat gacgatatct tggtagttaa
     2461 taaatcggcc ggcatggttg ttcatccggc gtatgctaat tataccggta cgctggtcaa
     2521 tgccttactg catcatgtgc aggatttgtc gggtatcaac ggtgagttac gtccgggtat
     2581 agtgcatcgt attgataaag acaccagcgg tcttttatta gtcgccaaac atgatcgggc
     2641 gcaccgtcat ctgagtaaac tttttaaaga tcataatatc gagcgcgaat attgggcgct
     2701 ggtatggggt aaacccaaaa agaaaaaaga tacgattgaa accctgatcg gtcggagtac
     2761 gaaggatcga aaaaaatttg ccgtgactaa ggaaggtaaa aacgctatca cgcattacga
     2821 attgataacg gcgtacgaaa gcgtttcatt gattcgatgc cggctggaaa ccgggcgaac
     2881 gcatcagatc cgcgtgcata tgaactatat cggtcatccg atacttggtg acaaaacgta
     2941 tggcggagat aatccgaatt tggcgggcgg agaaaaaaag aaaaaacagc acgctgaaaa
     3001 tatattagcc ctgataccgc gtcaggcgtt gcatgccaaa acgctgggtt ttattcatcc
     3061 gacaactaaa aaaatgatgc gctttgattc ggagctgccg gatgatttta atgccgttct
     3121 tcaattgata aaataatttt ttagcccgaa taggttgttt ttttgtataa ttgtcacagt
     3181 cgtaaatcgg ataactaatt cgcatgcgct acattcgagt gtatttattt gtaatgattt
     3241 ttgcgggcgg ccttcgttcg tatctgtcgg ctcaggaaat ttcgctttca ggggacctgc
     3301 aaccgttttc ggttacagcg cccggtgcga tttctgatga gttttcgtat gccgtgtcgg
     3361 ctacgggttt gagcggttct ttattaatac atgcgccgca tggatttgaa atctctacaa
     3421 ccagcggagg cgtgtttggt tccaatatta cacttcctga aaccggcgga gaaattgagc
     3481 ttacgacaat ttatgtgcgt tttgcgccct cggttaccgg atcgtatgac agtgtgatcg
     3541 tacatgaaag cggtgttaca cagacactgg ccgtaacggg tttgtgcgca ttcagtgaac
     3601 cgacattgcc gccatcggat gtcgtattta gcgggattga ttcatcagcg atgaccatca
     3661 cttggcaggg tggtgacggc gactatcatt tggtacttgt tcgcgccgat gcgctcatcg
     3721 agcttgcacc caatgacggc caaagtatta cagcgaattc gacgtttggc agcggtacgg
     3781 atattggtgg taatacctac gctgtttttg caggcagcgg aacgtctgtg agtatcaacg
     3841 gtttatcgcg cggtgtgaat tattattttt ctgttttcac gtataacggc acggggaatg
     3901 atgcggatta tctgacggac gcggctgtcg gtgaacagac gacgctgagt cgtccgcccg
     3961 aacggataca aatgatacgg gatactacat ttgcagaaga tagcggcact cgtacgatca
     4021 tagcggatct ccatgatatt ttttccgatc cggaaaataa tttgcttacc ttttctgccg
     4081 gcacattggt atcgggagcg gatgcgtggg tcacagataa cacgctggag tttttttctt
     4141 ccgaagattc atccggtcta tttgaagtga tcgtcgaagc gcaggataat tccggtaact
     4201 ttgtatacga tacgctcctt gtcacggtaa cgcaacaaaa tgatgcgccg aaattagcta
     4261 ctccgatcgg gccaaaaaat ttgcaggaag attttggaaa acaattgtta gcctatctgc
     4321 cattggtatt tcatgaagcg gatggtgaag cgatgacatt ttcaatacat acgccgtctc
     4381 cgtatgcagc gttgatgatt tccggcgaca gtttgtatgc tttgtcacaa aataatctca
     4441 acggtcaaag tcaggtaatc atcgaagctt ccgatcccac gcctttgagc aatcgtgaca
     4501 cattccaact tcaaatcatt ccgatcaacg atccgccggc gcgtacgtcg gtgttgcggg
     4561 acaccagttt tgcggaagat ttttcatatc gtccgttagt ctatctaccg gattattata
     4621 gcgatccgga tgatggtgtg attaattttt cctcttttgt tgtgaatacc ggcgttgagg
     4681 gttatatcct taacgattcg ttatacgtgg aaggaaatga agattttttt ggcagcgctg
     4741 ctcttgaaat tcgcgcttca gatggtaata cggaaatacg ggacacaatt ttttttacaa
     4801 taacaccagt taatgatgcg ccgagtagaa tatttatagc ccgtgatacg agcgtacaag
     4861 aggatgcggc ccgcacggcg attttgtatc tgccgatgat ttttttggaa ccggatttgg
     4921 aacccgtcag ctatggcgta gcgctttcca atattaagtc ggtcgcggca atttcgggcg
     4981 atacgttata tgttacgccg ctgaaggatt ccacgggttc ggtggaggtg tatttgtcgg
     5041 ctacggatcc gactgatttg tcagccaaag atacgctggt cttgcaaatt gtaccggtta
     5101 acgatagacc tgtacgcttg gcgacaatat cggatacgac gctaccgcaa aatttcggta
     5161 aggttctgat caaaaaactc tcaaccgtat tttatgatgc ggaccatacc gcgctgagtt
     5221 tttcattcgt caaatttgcc gatggtgtag atccactgat ttccaacgat tccttatatt
     5281 tgcgcagtac agtagatttt tttggtaacg tttccgtgcg cgtcattgct tccgacggtg
     5341 aatttacggt atcggataca ttccgtgtcg tagtgcagga caatacggcg ccggtggcat
     5401 ttctggcggc attatcatcg ccgttacttc agcgtattcg gtttgcattg ggaagtaatg
     5461 aaaccatcgc ttcggccgcg cttaccgcca acggacaggt gattgatatc gagtcaacca
     5521 atggtgtgta tttcggcgac tacagtatcg aatccgtcgg tgtaataccg gtcgtgggtg
     5581 aagtatttga tcttgccgga aataaggata cgattcgtcg aagttatacg gtaacttctt
     5641 tggataaaaa aacaacctac gacaattttg tttttgaagg taatcaacgc ggatatctcg
     5701 ttttggatca agctgaaacc ggcaatgtcc cgacgttgtt acgcccgctt tcgaatgcgg
     5761 tggatgtact gaatacggct tcctcagctg aagttaccgt gcatgtccga tatcaggatc
     5821 atctaagtgc gttgcgtgcg gcggatgaaa attttgatga agggcgtatc ggtgtctatg
     5881 cgtacgaaaa tcatcaatgg cagcgccttt ccggtgaagg tcgttttggc gcggttgaaa
     5941 cacgcattca taaaaatgta cgtatcgcgg tgttttatga ccccgaatat atcatgcaac
     6001 cgaaaacatt tgcccttgat caaaattatc cgaatccgtt taatccgtcc acgaccattc
     6061 gttttgcgat ttcacaaccg ggacatacga cgttaaaagt ttataatatg ctcgggcagg
     6121 aagtgcgaac acttttcagc gaatacaaac cggctggaaa ttttgaaaca atttgggatg
     6181 gtaaaaatga aaaaggcctc gcggtagctt cgggcgtata tttgtatcgc ttaagctcgg
     6241 gctcatttac gcaaacacgc agaatggttt tagtaaaatg aaaactttat ctatccgtta
     6301 tttgatttta ttggtttccg ttttttggat tacaggttgc ccgtccaaag atgaaacggc
     6361 tatcgaaaag gcgcagcaat tatttgaaga cggtgattat gaagatgcgt taagtcatta
     6421 tgaaagtttg attgcgaccg aaggttcgac ggcgcgtgtt ggtgcaggtt ggtctgcgat
     6481 gcggctcaac gatctgaata cggcgaatgt ttatttttcg gaagcggcct tggattcgat
     6541 accggacgga tacgccggtt ggagtgttac actgtggggt tccggaagtc ttttgcagtc
     6601 attggacaaa acagcctttg tgttgcgaaa aaaaccggag ttcaagctct cgcttgatcg
     6661 tagcgtgaca cataaagacc tgatatgggt tcaggccagc tgttattatc agttaggaaa
     6721 ttatcctgcg gcggtcaata gaattcaggc gttggatgat acgtttgctc cgaatatgga
     6781 ggcggccaat ttagcggaag tcatcgcatt aaaacttcag gcattgggtg tcgtacacta
     6841 gaacaatatt attccgcacg tgcacgcgga tcggctacgg catacaatat atcagctaaa
     6901 agattgccta ggataactaa tataccggcc atcatggtat tggcaataat cagcggataa
     6961 tcccgtgcaa aaatcgcatc aataataacc cgtcccatcc ccggccacgc aaaaataact
     7021 tccgtaacga cagcgcccgc caataaaaat ggaaacgata atccgatgat cgtgatcaaa
     7081 ggtagtaacg cgttgcgtaa accgtgtttc catattacca aagactcagg caatcctttg
     7141 gcacgcgccg tgcggatata atcttgctgt aaaacttcca acatactact gcgcacataa
     7201 cgtatcgtcg aagcggcaga agcaatcccc aacacaaaaa ccggtaacac catgtgcgcc
     7261 agcaaatcct gcacgcgtgt ccaaaagggc cacacttcgt aaccgatgct gtgcatttgt
     7321 gaagccggga accagttcaa ataaagtgaa aaaatcataa tcaaaaccag agctaaccaa
     7381 aaatgtggca tcgaataaaa aaacaaacct acgacggttg tcattcgatc aaaaatcgta
     7441 cccctgcgca atgccgaaac cacaccgagt ccgacgccga caagaatatc cacgatcaat
     7501 gcagcaacgg ttagtatcat cgtattgatt aacgcgtccg aaatcacttc tacaacaggc
     7561 atttgtttgc taaacgaaat acccagttcg ccttgtagta agccttcaaa cggcggtatg
     7621 acacccaacc attttccgta ttgaacgggt aaaggatcat tgaggccgag gttttcttta
     7681 agttgagccg ccgcttgcgg atctatggat gggtcaataa aaagtgatgt cggatcgccg
     7741 ggcgctaacc gtattaaaaa aaaagtgacc gtgagcaatc cgaaaatcat cgggatcgcc
     7801 gcgcgcccgc gttttaatat aaatctcaac atacggctat gggttttctt cgatatacca
     7861 ttcccaaaaa cggtggtatg gcgaataaat gtctgcgtgt gtgccgcgga cacgattatt
     7921 aaacacagtc aaattatcat accaaaataa aaaagttaca ggttgatcgc ggtaaataac
     7981 ggattgcaat gcgcgataag cgtcggctac agagtaacga tctgctgcgt tggcgataac
     8041 atttagtaaa ctgtccgctt cagcactctg ataacctgat gcgttaaatg tatttttttt
     8101 cagatcggaa ccgaattgcg gttgcaggtc aaggctcatg ttgaccgagt agccggtgat
     8161 gaatgcatct aattttcgct cacgtaacgc ggcattgaag gctgccgttt cgagcatctg
     8221 gatttctatt ttgatgccga cgcgcaacag atccgattgg attttttgtg cgataaactc
     8281 gcggcgtgga ttgccggtat tggttgtgag tgtaaattga aaaggtttac catttttatc
     8341 aagaataccg tcaccgtcat gatcggtcca tcctgcactt tgaaggataa tgcgtgccga
     8401 atccgggtcg taggtcatct ccggaagttg atcgttatat gcccatagga aaaccggcga
     8461 taccggacct gatgctgttt ggccgtattc tccgcaccaa ctttgtgtga tctctgcgcg
     8521 atgaatcgcg taactcaatg ctttgcgtac ctgttgatcg gaaaaaagtt tgtgcggttt
     8581 tatcgttccg gagctaatat aggacgatgg atcaatatga ttccacccta caaactcgac
     8641 acggcgcccg gattgacgtt cgattctaaa acgggattgc ttgcacagct cggcggcatc
     8701 ggacgggtta atgccttcga caacatccac acgtccggat tgtaattcga taaggcgcgt
     8761 ggtcatatcg ggaataatac gaaatacgat ctgatcaaga tgggctttgc cgggtatcgt
     8821 ccattccgaa ttgcgtttga gaatcaggtt ttgttttcgt tcccatcgtt ccaaggtaaa
     8881 atgttttccg ctcaccggtt tctgattaaa ccaggatgta cgaagacgcg aagacgggat
     8941 tgtatccagc acgtgtttgg gaacgggggt ctgttgtgcc cattcggtca tacggtggcg
     9001 tggcgtgttc tttttgaaat aaaaaacgat cgtggagtca tccggcgtgc gaattacatt
     9061 ttcccaatca atcgagccat cgtcacgggt ttgtacgata tcgattcgtg ttcgccttgc
     9121 actcccaatc tgcggatcga agtataaacg ataagtgtac gcaaaatcct gtgccgtgat
     9181 tttttttccg tcatgccaat gtgcatgcgt atgcaaatgg taagtcagcg tagaatggtc
     9241 atccgaaata gtccaggatt ttgctaatga cgggagaatg gtgtcgcgtc cggtggtttc
     9301 attaaagtcg tattctacca agtcgggata taaaaaataa atcactgcgg aagaattggt
     9361 acccgtggca aaaagaggat ttagcgtttc aggatcggat gacaaagcga tagttgtgat
     9421 cccgccgtcc gagggcgtgt gcggcacaca tcccatcaga tggataatca ccaaaataat
     9481 aagccaccgg cgcatggatt tataaaacaa aataagtgac ataaatatag gtgagcggtg
     9541 caacaaacat catactgtca aaccgatcca gaatgcctcc gtgcccgggg agcaaagcgc
     9601 cactgtcttt gatgccggca tcccgtttga gcaaagattc ggcaagatca ccgatctgtc
     9661 cgaatgtacc gccaaaaaaa ccgattacca atgtttgcgg caaactcata atgaaaggga
     9721 tgtcaagcgt cagatagatg tagcgaacga gccatacgcc gatcaatgca ccaaaaaagc
     9781 cggtaatggc gccttcccat gtttttttag ggctgaccct ttcgaaaaat ttatttttac
     9841 ccatcattcg gccgccataa tacgcaaatg tatcgcaagc ccaaatgctc aagaaaacca
     9901 aaaacactaa ttgagcgccg ctatgactct ccgaagcgcc ggtattacgg agtaaaatca
     9961 tcgagccgat caataatgac ggataaaaaa cggtcatcca tgtggccgcc atattcatca
    10021 tatccgaacc ggtgttgcga aacaattcca cggtcatggt atatattgca aacaaaaaaa
    10081 gggcggataa taaaaactca attttgccga aaaacgtgag ccctagaaaa atcaatcccc
    10141 aaaaaaaagc tgagaaagtt tgtggtttac tgtttttgtg gcgaagcagg tttagcattt
    10201 cggatatgcc gatgagcaat atagtgccca tcagtgcaaa aaaataccaa gaaccataaa
    10261 tgatggcggc gatgacaaaa ggcaaagcta caatcgcgac cacgatgcgg gcgagtcctg
    10321 aattcacata aacctcgttg tttgatgtac aagaccgttg attgaaaata aaattatgtg
    10381 atgtgcatca aaatatagat cgccgtataa ggaatggcaa gtaaaatacc cggcaatgca
    10441 ttaaaaaaaa agcccatatc gtcaatcgat acgggctttt aaaaactaaa tttgaataga
    10501 tcaggcgatc atggtgccgc agttaccgca gagttttact ttgcgttcca aaaatttcca
    10561 tgcaccggtt ttttccgata ctttggattc tacgaatttg gcgtacgatg caacagattc
    10621 ctgtttgcag gcttcgcatt ttactttttc agcatacgct gcgactttag cggttttatc
    10681 aacaaatgtt tgtttctttg ccatggcaaa gctcctttac aaaaaatgct tattaacggg
    10741 tttccttgtg cagggtatgt ttacgttcta cattgcagta tttttttact tcaaggcgtt
    10801 ccgtattatt ttttttgttc ttttccgtgg tataacggga cataccgggc aaaccgctgt
    10861 ctttgcagga tgtgcattcc agcgtaatca cgatgcgatt gcctttagac tttgccatga
    10921 cgaactctcc aaaagactat gtttataata aaattatatt ctatccaaaa tgggcagaaa
    10981 tatatattaa aagctttcgt ttgtcaagta ataatattga tttttcgtgc gctgtttttt
    11041 ataggtttag gtgattttga taagcttaat cttgcgatta gttatataga attgtatttt
    11101 ttgatgcgct ttagatttca atggattaac attttacaaa atcggtttga gtaactgaat
    11161 tatgattgat tttgcagaac atgccccttt acaaagtgag tcagggcgtt tagtattgcg
    11221 cgatccttat ccggaagatg cggcgactat ggtgatgtgg cgcaatgaat ccacggtaag
    11281 agcgcaccag ccattgcgta cggtcaccgt ggacacgata gagcaggatt tgttacgttc
    11341 gcagtccaat gttttacccg attataatcg tgagcggttt cagtggatca tagaaaaaac
    11401 agaagatcgt atatccatcg gctggattac gctgacgata cgttcctggg aacacgaaat
    11461 cggagaaatc ggttacagta ttgcgaccgt ttttcaggga cgcggttacg ggacggaggc
    11521 gatacggctt gtgttaaaaa aaatatttta cgaagcccgt ttgtaccggg ttgaggccaa
    11581 atgttctgtc gaaaatatgg cgtcttcacg tttgctggaa cgattgggtt ttcatcgtga
    11641 aggtattatg cgcgaatatt ttgatatcca tgggcgacgg gtggatcatt acttatacag
    11701 tctgttacgg cacgagtatt tttccaagct gtaaaaattc attgtatttt tacatgcggt
    11761 catttagatt gcgccatcgt taaatataaa aatatcgttt taaaatattt tattggccgt
    11821 agattaaatt tagagtgcgt cccgctcgaa gacgggaagg tcagtggttc gatctggtaa
    11881 atttttaaac gtatctttga aattgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnngcc gtgggcgtga ggagagcgtc ccgcttgaag gcgggaaggt cagtggttcg
    12061 atctggtaaa tttttaaacg tatctttgaa attgttttga tattggggct gtagcttcac
    12121 gccgtgggcg tgaggagagc gtcccgcttg aaggcgggaa ggtcagtggt tcgatttggt
    12181 aaatttttaa acgtatcttt gaaattgttt tgatattggg gctgtagctc agcttgggag
    12241 agcgcatcgt tcgcaatgat gaggtcaggg gttcgatccc cctcagctcc acatggttta
    12301 cgttttgtat tgcggaacac aaacgaggca aattctatcc actcgttcca tagttttatt
    12361 gactatgatt tactaagaac tctatcctat gaaatcaagt ggaagaaaaa gtgctcatct
    12421 tatttagtta ttcttaggag agcgtcccgc tcgaaggcgg gaaggtcagg ggttcgatcc
    12481 ccctcagctc cacgtcgaaa ataataaaaa ccaatgaaca aagaagttta ttggtttttt
    12541 gttttatctg ttgccaacga aaaaacatac tgcatattaa ccttcgcgga gcacatcagt
    12601 agcacgtgtt ttaatttgtt caagtaccgc tatggtcagc atcaatatcc caagttgaat
    12661 tacaggaacg aattgtgtac gttggtttta ttaagaggaa cactccgcaa tgatgacctt
    12721 tcctgcaagg tggacaaacc atttccgtgt tgcaatctct cagttactgc ttttcgccgg
    12781 tgtcgtgtat tttctttttc gttgggcgga agaagatcgt gcattgtatt tagttttggc
    12841 gggtataacc ggtacggcct tttgtgtgct ggcgctgttg cattttacta aaacagcacg
    12901 cattgcacgg cgcatgagtt ccgtaatcat tatcgctatg acattttgtt ttgtaatcgt
    12961 aggttatctt tttcgcggcc atatcgatca cgtgatacag gaagcggatt tgtcggccaa
    13021 ccttgatcgt cttgcgtcct atcgggccaa cttaaacaat cgctttttga agcatgccga
    13081 gcaaggcgag tttttgagaa aacggcttac tgcggaaccg ttttggaggc aaccgggtat
    13141 attagccgaa ttcctgaggc atcataccga cgactatttt tctgtttatc tcttagacga
    13201 tcagggtgta agtcttttgt ctgaaccggt atcaaccgga tacaaagaaa gctaccccgt
    13261 tcgtaatttg tccaatccgg cattcgtcga accgtcattc caacctataa acgatggcaa
    13321 taaagtccca gcgtggcatg cgctcaaata ttttatccct cttgaaaccg cagaaggtca
    13381 aaaacgttat gttttactcc atatcagaac gaatgcttgg ctccatgaaa taaaacaatc
    13441 catgccgggc gtttctgttt ttttgctgca tgataacgag atcatcgcga ccaacgaaaa
    13501 acactggaat gaaaacataa tacgcgaaat aacaaacgaa ggatatgggc gtaattccga
    13561 agaacagatt ttttttgcgc gatacccgct gcgcagtgat aactggtcgg tggttttttt
    13621 acagacatgg aatacggcct ttcctaatgt gtcatacgcc gaatcggcat tggtacgtgt
    13681 gctgattttt tcggccgtat ttggattttt gaccggagtt ttattgagtt ggtatctcag
    13741 tcgtactatt cttttaccct tgagccggtt gacaaacgat gttcagattg ttgtgtccgg
    13801 tgatcaaacg catacacttc gcgaatcttc ctctgatcca caagatgaga tcggcgctct
    13861 tcatcgcgct atagcaaaca tagtaaaatc cctaaaggaa agaacgatac aaaccgaatc
    13921 cgttcaaaac gcatggtctg atgtgcaatc ggaattggag gctcaatccg atgtcgtaaa
    13981 aaaaatccaa gccgatactc ttaggtcatc gcctttaata acgcgtagtc tgacgatcaa
    14041 tggtcgttta gtaatagccc aagagcacaa cggaaaattt tacgatataa ccgaaccgca
    14101 accggggttt gctgcttttg ctatcgttga tcctgccggt aaaggtgcca tcggttcgtt
    14161 ttatgctgca ctgatgaagg gattgctgag tgcgtattgg aaacgcagtg atttgcttct
    14221 gcaagaccct ttgcgatgga tgaacgaact tgaaacggat tttatgaaac atcgggatac
    14281 cgatacgcga acgatagcac ttagtgtcgg aatcattaat acccacgacc atcgagtacg
    14341 gttgatcaat gcggggtcgg aataccccgt tttgcgaaaa aacgatcaaa cgcaatctat
    14401 cgccatacgc ggcagagctc tgggcatgcc ccaatggctt ggttcttttg aaactacgac
    14461 atttgtattg gctttgggtg aaactatgtt tttacacagc catggaattt cgcccgacgc
    14521 gatgaccgag tggccggaga aaattcataa cacggcgatg gattcgaaac agagtgacgc
    14581 cgtcatatta aaaatagttc gctgtgaaag cgttaaagag aatttcatat ttacaccggg
    14641 gtcggatgta gattttgacg cggaaaataa aatccaaacg atgatggcgc gtcgtggttt
    14701 tgaatcggaa aaaatcaagt ccatgttatc cgcttgcaac gcaacgataa cctatgctgc
    14761 ccggagtacc aggctgcctg agttgaaaaa ctcgatttat gtcgaagctg aagcgtgtga
    14821 tgacggcatc gaagtgcgaa tcacggatca gaataacaat tcgtattgga atatggcgag
    14881 gatcccggaa gaattcgatc aaaccgaaag caaagggggg cgtgcgtgga atatcggtgc
    14941 catacaaaaa ctcgttgatg aagtaatcgt agagcaatcg gctaataaaa caacgatagt
    15001 tttacgatca ataaaataac gactatttgg atgcaacgat tttggcctgt tgggcaagtt
    15061 cgtttttata gagtctttct acttcgtcaa tcaatccgac aactttcgag tcatgcagat
    15121 aagtattcag gtcatcttgc aaaaccttgg catccatttt cccgccaatc agattatctc
    15181 tgcgcgtacg taaaccgaca atcacttcat ctaagcgatc tacgatatct gtggtgttat
    15241 taagtgtgat agcgctttgt atcgtttcca aacggaataa ccgttctgtc aatgtcgtaa
    15301 gaatatcatg tttgacgcgc gactccacgg tgatctgttt agcggccatt tccgcatctt
    15361 tgcggagttg agcgatagtc tggatgattt cgtcaaggga aacagtttta cttttggcat
    15421 gatcttgcat ggcggccaga tgcgctgtga gaaataccac gctgagactt ttgatggaaa
    15481 tttcctgaat accgttgatg agctgttgca aagtttccat catctgacgc ttggtcatat
    15541 cgtgatgacc ggagatcaaa tactcgcgct cgatataaat ctccagcatg atattgagtt
    15601 caaatgtgtt gatgagttcc ggccagtatt tatccggttc ggaaagcgtc aacagcaatc
    15661 ccggattttc gaagatatgt tcttctacca gatccgtgat ggcaaactga cggtgcatgt
    15721 catggcaatg ggaaacgcgg gttttgatag actcaagatg tttgtcccgc gggccttcgt
    15781 aatcttcgag aaaagctttg aggcgcgcat gcaaataggt gagaatcaat tgttccaacg
    15841 tcacccgttc acctgttttt tcgttgagat ctactaattt gcgcagcaac gaacggaatg
    15901 aaataattgt tttgtggaaa aaatgattct tgtgatcgga ttc
//
LOCUS       DAOQGF010000077        15704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1716_length_15704_cov_7.296952, whole genome shotgun sequence.
ACCESSION   DAOQGF010000077 DAOQGF010000000
VERSION     DAOQGF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_15704_cov_7.296952"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PK937_10845"
     CDS             complement(<1..757)
                     /locus_tag="PK937_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNH33111.1"
                     /translation="MRVSLRWKFILGFIAFGAFLVATTSTVFYIAYRTAITGKIETDL
                     TSESQRFSEFVSFSNDSISVEDSDEWKELEHIKTSPYARYIIITDNNFKTLRKTGNLA
                     DVEFQSYHTFRPTPEVTAVLLDIENEKYVCVIYPIHRKGRPAAYVLAAHNFRIADRYI
                     DVFENTMIFSMAVVVLLGTIVAYFFVRRITKPMIEIIRVANTINLEHLDQRITLESPD
                     EEIQNLVTTLNQLLDRLHRSFNQINEFSSNVSHE"
     gene            complement(758..1429)
                     /locus_tag="PK937_10850"
     CDS             complement(758..1429)
                     /locus_tag="PK937_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH33112.1"
                     /translation="MRILLIEDDRKVASFIQRGLSEAGNAVDIVYDGRSGQTQAEVEN
                     YDLLIVDWMLPNKDGVSVCRDLRANGYTLPILLLTARETVNDRVTGLDAGADDYLTKP
                     FAFNELLARARALMRRGSRTLDLFTVDDLEMNLSERRVTRGGQGIELSNREFSILEYL
                     LRNKNRLVTRTSLTEHVWDIHFDRGTNIVDVYINYLRKKIDTGTRKPLIHTIRGAGYM
                     LKEPD"
     gene            1703..2182
                     /locus_tag="PK937_10855"
     CDS             1703..2182
                     /locus_tag="PK937_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33113.1"
                     /translation="MNPAHMHLLLNHIPVLALAAATILLIAARWRSQIEWTRLSMLFF
                     VIAGAVSVPVFMTGEPAEKIIENIGGISMMHLESHEDSAVASLIGACILGVLAVIGLF
                     FIERVHAHRLIWATFLIIALTANGLMAWTASLGGKIHHTEIHNGYTPAQTQKKYDDD"
     gene            2208..3179
                     /locus_tag="PK937_10860"
     CDS             2208..3179
                     /locus_tag="PK937_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNH33114.1"
                     /translation="MKRWIVYLLLCAGFYFLPVYGQENNEKDDARPSVSGYAQIIYSQ
                     YLKNQEHDRQSGFDINRVRFAFEGSVIPDRVHYAVEFDPQGSPILDDTYLDFIISKNS
                     KVRLGRYKIPFGAENPTGEDELHFIEKSKVVESVFSAREVGIGTYYERNWIIGDVGYF
                     QKSEDEHIERDVIGRIGLKPFGKYIETGGSGYYEKIHTDSTNFAIHRIGADLTCEVGP
                     IKMQGEYIRSKGTMEDKQLSGYGYYGYGQYTHHIGDTQISWGVRYEFYQPDKNNKSDQ
                     ERKYVVGSTIAFHPRMRAQVNFQHIRENIETNNQGQNKLIVQYQFSF"
     gene            3195..4292
                     /locus_tag="PK937_10865"
     CDS             3195..4292
                     /locus_tag="PK937_10865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH33115.1"
                     /translation="MFKKYSVLTAACIFGIGTFSVYLFGEWHEIQDEGLRWFLELPHM
                     TLSFFLIIMLPLAVHQMAILIERKNETRMLWTRFMVVVVLPYIVVATYLAAEWDEIEE
                     EWLPWLIKPSHFLIVLINGIMIPGILWLIINKFIQLWDERERFLKRIVELENELRSFV
                     HPEVYKRIYEKTSLEATGAFKIIMADMQGFTRFCEAHSAAEVANLANEFCNLMDERIR
                     QVGGITLSYAGDLVFALFDEHIASDDIVGVAKKMQETFRAFAQTKFPDWDTNLNIGIH
                     YSRDIVLGTIGGGSAQKHFTANGFGICEVSRMCSEADHGQILVSEAFAETVTSDPIRS
                     RLIGIAWSKQPKNITVPPVMFEYRLVDNEIT"
     gene            complement(4330..4836)
                     /locus_tag="PK937_10870"
     CDS             complement(4330..4836)
                     /locus_tag="PK937_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNH33116.1"
                     /translation="MKEYNVITTSASVVVIGIGNEYRRDDRAGIEVVRAIADMCLPDV
                     CVIEHNGDGSSLQDVWSDARWVIIIDTVCSDNIPGTIYRLRANADIIPGNFFNYSSHA
                     YSISEAIELAKALHHPVPEVIIYGIEGANFEFGEELTPNVREKIGDLITRVCQDIERI
                     MQESPVSA"
     gene            complement(4877..5335)
                     /locus_tag="PK937_10875"
     CDS             complement(4877..5335)
                     /locus_tag="PK937_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNH33117.1"
                     /translation="MKDLKFFLMQHPLLIDMSAEHVELIIGCASNVRFDTGQIIFREG
                     QEANEFYLIRQGKIALEIGSPGRGHIGIHTLTEGDILGWSWLIPPYHWQFDARALDLT
                     LAIALDGRCLRNKCESDHELGYQLLKRFSHIVTQRLESTRLQILDMIEHV"
     gene            complement(5652..6002)
                     /locus_tag="PK937_10880"
     CDS             complement(5652..6002)
                     /locus_tag="PK937_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNH33118.1"
                     /translation="MNLETIRTYCLKKKSTDEDFPFDQETLVFRVSGKIFALINTHIA
                     EFINLKADPEKAIEWRERYEGVQPGYHMNKKLWNSVYLDGSVQDKEIFLMIDHSYDEV
                     VKKLPKKERIKLQP"
     gene            complement(6698..7636)
                     /locus_tag="PK937_10885"
     CDS             complement(6698..7636)
                     /locus_tag="PK937_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNH33119.1"
                     /translation="MNAGITKARVDSLNPRKPLYVEKDATVADVVAMMEQTKSGCILV
                     RDKGALIGTLSERTILRNLIAMHMEFSHTPVTRLMSADTHCLYADDSAEYALNEMTFG
                     GFRNITVLSQVGDPSGILTFEDILDHILAKMHVRIDTEEKKPRSPEKKRAREKQGIDP
                     EDFDRPIGVLDPPAALCVESGSLLKDAVRQMADRNIGCVMVVRDKKLTGTVTERFLLY
                     KIAHETPDFDTAKVDDYMRVAPQCLQMHDPVSAAIRILALHDVRHIPIVNENGEPVAF
                     TSVRGVIDYIVSFYPDEIINLPPHPMRFGLRDEAGA"
     gene            complement(7717..8691)
                     /locus_tag="PK937_10890"
     CDS             complement(7717..8691)
                     /locus_tag="PK937_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNH33120.1"
                     /translation="MHRRQFLKTSGAMLLVCAGAGLGPLLLSRRRVYTMTRPLMGTIG
                     EIHVVSDDARWADRAMEAAFDELQRLESLLTYFSPVSDVGRINAATTGAEIRVASETA
                     DLIERALHWSKVTDGGFEPGLGKVSDTWDVKHRTEPPAQDVWQRFAGLSLHRFIHVRT
                     GSDTTVKILSPDVRLDLGGIAKGYAADRAAEVLKKEGITQALINLGGDIVALGRRADG
                     EGWRVGVRDPERADVMATVVTLHDQAIATSGTYEQYFRSGGRVYHHLIDPKLAEPKRA
                     QFQSLTIIGTNGRDTDALATGLFFSTDEDKKKILADHTDRFQCMTWGA"
     gene            complement(8708..8938)
                     /locus_tag="PK937_10895"
     CDS             complement(8708..8938)
                     /locus_tag="PK937_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33121.1"
                     /translation="MELAKYILPVFASAFLCAFWIIVQMIARRMKTKNLFDHKPSCGH
                     CDEDGSCGGDHCEARETLRKKMPWAGSAAPTK"
     gene            complement(8943..9521)
                     /locus_tag="PK937_10900"
     CDS             complement(8943..9521)
                     /locus_tag="PK937_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNH33122.1"
                     /translation="MGEYLWIFISALLINNFTLANFLGICPFLGVSNKIETAFRMGLA
                     NIFVMLITAICAWALNTYVLVYAPYLRLISFIVVIASTVQFLEMVIKKLSPELFRALG
                     IYLPLITTNCAILGIALFQTSKEYGLVQALFYALGGGGGMTLALVIMAGIREETRIMD
                     IPKTLQGTALSLIIAGILSMAFMGFAGLFSSN"
     gene            complement(9528..10154)
                     /locus_tag="PK937_10905"
     CDS             complement(9528..10154)
                     /locus_tag="PK937_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNH33123.1"
                     /translation="MNAIEKSGRATHELIKGLWKENPTFVAVLGMCPTLAVTNSAING
                     LAMGLATTFVLVGSCLLVSMLRHWIPKQVRITTFIVIIATFVTAVDQSLEAFFPKIHK
                     ELGAFIALIVVNCIILGRQEAFSYKNPPWLAVMDALGMGLGFLGALMTLSCIREVLGN
                     GSLFGVSLFGDAFEPWVIMILPPGGFLLLGSLLVVINWIKFGRQTAKV"
     gene            complement(10151..10840)
                     /locus_tag="PK937_10910"
     CDS             complement(10151..10840)
                     /locus_tag="PK937_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNH33124.1"
                     /translation="MEHQHIPATPVATKEAEPSSLILILTLAVAGFFSGLILVVAYFS
                     TLPIIEQNKEEALQEAIYRVVPGCRSYEVLVLKNDRLEKAQGEETKKGDRIFLGYDEK
                     KSVVGFAIPASEPGFADVIKIIYGYQADGKIIVGFEVLENKETPGLGDKIIKDKNFVA
                     NFKALSVEPEIEAAKTGKKTKPNQVETITGATISSKAVIRALQKAMTRWQVAIDGYMQ
                     QNNLTYNQKQP"
     gene            complement(10849..11850)
                     /locus_tag="PK937_10915"
     CDS             complement(10849..11850)
                     /locus_tag="PK937_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNH33125.1"
                     /translation="METQSKLTLSTSPFLHDEATTPWIMRQVIYALVPVAAAAYYFFG
                     LSVILLSVAAVSGCLFAEWLLSGSNPRHQSLFDGSALLTGLLLALTLPPGFPMWMAFL
                     GGLIAIMIGKYIWGGLGQNIFNPALVGRAFLQAAFPTAITTWSVPDGRYFSARGTNLA
                     WPFFHGEPLDAISSATPLSSMKFQHEATELSHLMLGHTSGSLGETCGVIILLAGVYLA
                     YRKIFNWHIPVSIIAATVVLSGILYIVNPILYPSPMHMLFSGGLMLGAVFMATDPVTS
                     PMTVRGMWIFGSGIGILVVLIRIFGGLPEGVMYAILLMNAVTPLINRVTRHRVYGTK"
     gene            complement(11829..13187)
                     /gene="rsxC"
                     /locus_tag="PK937_10920"
     CDS             complement(11829..13187)
                     /gene="rsxC"
                     /locus_tag="PK937_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNH33126.1"
                     /translation="MENMSSRLTFRHGIHPEAYKDQTADLAIERMPFVEEYAVPVAQH
                     AGAPSVPLVQKGQHVTRGDVIARPDAFVSVAQHAPVTGTVTGIELGEHPNGLLVYSIR
                     IKADPYSAQTVPQRPPLDWKNMGHKEFIAAVQSAGLVGLGGAGFPAHVKFSIPEGKTC
                     TTIIANGCECEPFLTSDHRIMVEFAADLVHGLTILRHFVPAQRVVIGVEDNKPDAIAA
                     LQEEIKKQNCDYEVVALRVKYPQGAEKMLIKAVLGTEVPSGKLPLDVGALVSNVGTLV
                     ALAQYFENAVPLIERVVTVTGPAIRKPANLMVPIGTPIRHLIDWCGGVTQDAVRILLG
                     GPMMGIAQKTLDVPVTKGISGVLVLTDNEVQELSTYQCIKCGRCIDVCPMFLNPSLMG
                     LLSRKDLYDELEEQHVMDCFECASCSFVCPSGIPLVQSFRVAKNAIRERKAKASSKPN
                     PN"
     gene            complement(13187..>15704)
                     /locus_tag="PK937_10925"
     CDS             complement(13187..>15704)
                     /locus_tag="PK937_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH33127.1"
                     /translation="KSSYPKQQIHFQNLHREFPDLNARSIGLDESAKTMTAISSDIPG
                     IVRRYEDRGAPYSRVASFYDRVGFFTESGQTKAMTADPFHALPVIPPATSAFHNATAQ
                     RAMYPVLKSESCTGCGQCAVYCPESALPMVALSTEALVQSAVEQTIRAGKSLTEMTPP
                     VIKNLAKLLHPMVSENNPSLCDLLPVAAEKLAVQMKMDSSKKENLIAQATDVGQYLAP
                     LPAVITKTFFKTPEQYAKGSGLIFSLAVDPHACSGCGICAASCSEQALSMQPVNPASE
                     AQLQASYRIWEALPDTPGATVKQYLDNESYNPLAALFLSRHYYMAQLGSRRTDQPAEK
                     IALHLITAAVEAATQARMLTHTSAIEKRIQNLTDKLQNILRDALPTQSTEHLLAAVSE
                     HGTERMSLDALIGKLGSSQRFGVVDTAWIERVVYLLRDLKQLHWLLTKGPTGIGRARY
                     SAVISGNDTLAWARQFPDNPFLVPVMVFSEAIAPDFVRGIGLGQMRHIIDNIKLLRRA
                     DLELQNAYLPSQHDIQIAAITWNDLTEEEKNLAPPLLLVTDATVRDAMPAGDIIALMN
                     SGLPIKWIVTEPATGGKQNMYDRMGLNAMLFAQRQTAWAHVSPGYPADMYRSITACLR
                     SQTPAMMCVHTPMMIEKFKLQDMYAMSVESRVFPLLRYIPDAKKKLAAELLHLDHNPD
                     YQSDFVTGKNVTFADWAWFQPMWKSHFSAVSKPSSGSLDIRTWLASAEERAKHTAYYE
                     REGVFYLLSGVLAETCAVVYDAWLQLREMAGLLSAHPVKLRAHWDAEWSEKHNSALDT
                     LRSEYESKLKQQESNHLESTRIKLRDKLLALSGYQASGKQ"
BASE COUNT         4077 a         3784 c         3820 g         4023 t
ORIGIN      
        1 attcgtgaga gacattggac gaaaattcgt tgatctgatt aaaagagcga tgaagacgat
       61 ccagcaactg attgagcgta gtgaccaggt tttgtatttc ttcgtcggga ctctccaaag
      121 tgatacgttg atccagatgc tccagattga tcgtattggc tacacgaata atttcaatca
      181 tcggtttggt aatgcgtcgt acaaaaaaat acgcaacgat ggtgcccagt aaaacaacga
      241 cggccatcga aaaaatcatg gtgttttcga aaacatcaat atagcggtca gcaattctga
      301 aattatgcgc ggccaaaaca tacgcggccg gtcttccttt gcggtgaatc gggtaaatca
      361 cacatacata tttctcattt tcgatatcca ataagacggc ggtcacttcc ggcgttggtc
      421 tgaacgtgtg ataggattgg aattctacat cggctaagtt acctgttttg cgtaatgttt
      481 taaaattatt atctgtgatg atgatataac gcgcatacgg tgaagttttg atgtgctcaa
      541 gttctttcca ttcatccgaa tcttccacag agatcgaatc attggaaaaa gaaacgaact
      601 ccgaaaatcg ttgcgattct gatgtaagat cggtttcgat ttttccggtg atggccgtac
      661 gataggcgat ataaaaaacg gttgaagtcg tggctactag aaaagcgccg aaagcaataa
      721 agccgagtat gaatttccat cgaagcgata cgcgcatcta atccggctcc ttgagcatat
      781 atccggcacc gcgaatagtg tgaatcaaag gcttacgggt tcccgtatca atttttttgc
      841 gaagatagtt gatatagacg tccacaatat tggttccccg gtcaaagtgg atatcccata
      901 cgtgctcagt cagtgaagtg cgcgtcacaa ggcgattttt gttgcgcagt aaatactcaa
      961 gtattgaaaa ttcacggttg gataattcga tcccttgtcc gccgcgggta acgcgccgtt
     1021 cggaaagatt catttccaga tcatccacgg taaaaagatc cagcgtacgc gatccacggc
     1081 gcatgagcgc gcgcgcgcgt gctagaagtt cgttgaacgc aaacggtttg gtcaagtagt
     1141 catcggcgcc ggcatccagt ccggtgacac gatcgttgac ggtttcacgc gctgtaagca
     1201 ggagtatggg caacgtgtaa ccattggcgc gtaaatcacg gcaaacggaa acgccgtctt
     1261 tattaggtaa catccaatcc acgatcagca agtcgtaatt ttcgacttcg gcctgtgtct
     1321 gaccggagcg gccgtcatat acgatatcca cggcatttcc ggcctcgctc aatccgcgct
     1381 gaataaagga tgccactttg cggtcatctt cgatcaataa aatgcgcata acaatacctt
     1441 ttcgattgga aaaatttaca tctaaaacag acgtaatgca acgcattgcg aattaaaaaa
     1501 cattaatgcg catgtaggat gatatgctct gcgtcgcccc ctcgtaaagt agaaggtgtt
     1561 gaaacgaaaa aaggattata ccgtcgtcca tatacattaa aaaacttagg actcattctt
     1621 aaaactattt aatgcttcat taatctggtt ttaatgcgag gtatgtacat tgggatcaat
     1681 cttatcaaag gagaagcgaa ttatgaatcc ggcgcatatg catcttttat taaaccacat
     1741 tccggtactg gctttagcgg cggcgacaat cttattgatc gcagcgcgat ggcgatcgca
     1801 aatagaatgg acacgattga gtatgctctt ttttgtaatc gcgggtgcgg tatccgtgcc
     1861 ggtttttatg accggtgagc cggcggaaaa aatcattgaa aacatcggcg gtatatccat
     1921 gatgcatttg gagtctcacg aagattcagc ggtagcgagt ttgatcgggg cttgtatttt
     1981 gggggtactg gccgtaatag ggcttttttt tatcgaacgg gtacacgctc atcgcttgat
     2041 atgggcgacc tttttgatca ttgcattgac ggccaacggt ttgatggcat ggacagccag
     2101 tctcggtggc aaaattcatc atacggaaat tcataacggc tacacaccgg cgcaaacaca
     2161 aaaaaagtat gatgatgatt aaattatttt ttcaggagaa ctaaattatg aaacgatgga
     2221 ttgtttactt gctgctctgc gctggctttt attttttgcc tgtgtacggg caagaaaata
     2281 atgaaaaaga cgatgcccgt ccttcggtat ccggttacgc acagatcata tattctcaat
     2341 acctcaaaaa tcaggaacat gatcgccaga gcgggtttga tataaatcgt gtgcgttttg
     2401 cttttgaagg aagcgtcatt cctgaccggg tacattatgc ggtggagttt gatccgcaag
     2461 ggtcaccgat cctggacgac acgtatcttg atttcattat ttctaaaaat tcaaaagtcc
     2521 ggttaggtcg ctataaaatt cctttcggcg cagagaaccc gaccggtgaa gacgaactac
     2581 atttcatcga aaaaagtaag gtggtcgaat ccgtcttttc cgcacgtgaa gtaggcatcg
     2641 gtacatatta tgagcgaaat tggattatcg gcgatgttgg gtactttcaa aaatcggaag
     2701 atgagcatat cgaacgcgac gtcatcgggc gaatcggtct taaaccgttc ggaaaatata
     2761 ttgaaaccgg cggttcgggg tattacgaaa aaattcatac cgactcaacc aattttgcca
     2821 ttcatcgtat cggcgccgat ctgacgtgtg aagtcggacc gatcaaaatg caaggcgaat
     2881 acatacgctc caaaggtacg atggaagaca aacaactttc cggatacggg tattatggat
     2941 acgggcaata cacccatcat atcggtgata ctcaaatttc gtggggcgtg cggtatgaat
     3001 tttatcagcc ggataaaaac aacaaatccg atcaggaaag aaagtatgtc gtaggttcca
     3061 cgatcgcatt ccatccgaga atgcgggcac aggtaaattt ccaacatatc cgtgagaata
     3121 ttgaaaccaa taatcaggga caaaataaac tgattgtcca ataccaattt agtttctgat
     3181 cagcgaggca ttgcatgttt aaaaagtaca gtgtactcac agcggcttgt attttcggta
     3241 taggcacgtt ttcagtttat cttttcgggg aatggcatga aatacaggat gaaggtctgc
     3301 gttggttttt agaattaccg cacatgacct tatctttttt tctgatcatc atgcttcctt
     3361 tagcggtcca tcagatggct attctgattg agcgtaagaa tgagacgcgg atgttgtgga
     3421 cacgatttat ggtcgtcgtc gtgctgccgt atatcgtcgt ggcgacctat ctggccgccg
     3481 aatgggatga aatcgaagag gaatggttgc cgtggctgat caaaccttct cattttctga
     3541 ttgtattgat caatggaatt atgattccgg gtatcttatg gctgatcatt aataaattca
     3601 ttcagctttg ggatgaaagg gaacgttttt taaaacgcat cgtcgagtta gaaaatgagt
     3661 tgcgttcatt tgtgcatccc gaggtgtaca agcgtattta tgaaaaaaca tcgctggagg
     3721 ctaccggcgc atttaaaatt atcatggcgg acatgcaggg attcaccagg ttttgtgagg
     3781 cccattcggc tgcagaggtt gccaatctgg ctaatgaatt ctgtaacttg atggacgaac
     3841 gtattcggca ggtcggcgga atcacattat cctatgccgg tgatttagtg ttcgcgcttt
     3901 ttgatgaaca tattgcaagt gacgatatcg taggggtggc aaaaaaaatg caggaaacgt
     3961 ttcgtgcttt tgcacaaact aaatttccgg attgggatac gaacctcaat atcggtattc
     4021 attatagccg agatatcgtg ctgggaacga taggcggagg aagtgcacag aaacatttta
     4081 cggccaatgg gtttggcatt tgcgaagtgt cgcgtatgtg ttcggaagcc gatcatggcc
     4141 agattctcgt ttcggaagca tttgcagaga ccgtcacgag tgacccaata cgatcacgtt
     4201 tgatcggaat tgcatggagt aaacagccca aaaatattac ggtgcctccg gttatgtttg
     4261 aataccgttt ggttgataat gaaataactt aaacggtaag ggtggaactc aaagtttttt
     4321 gatacctgat tacgccgata ccggagactc ctgcatgata cgttcgatat cctgacacac
     4381 gcgtgtaata aggtcgccga ttttttcgcg aacattgggc gtaagctctt cgccaaattc
     4441 aaaattggcg ccctcgatcc cgtaaataat gacttccgga acgggatggt gtagtgcttt
     4501 ggccaattcg atagcttcgg aaatactata ggcatgcgaa gagtaattaa aaaaattgcc
     4561 cggtataata tcggcgttgg cccgaagcct gtagatggtc cccggtatgt tatccgaaca
     4621 cactgtatct atgatgataa cccatcgtgc atcagaccat acatcttgca gcgaagagcc
     4681 gtcgccgtta tgctcgatga cgcacacatc cggtaaacac atgtctgcta tggcgcgcac
     4741 cacttcgata ccggcgcggt catcccgccg atattcattg ccgatgccga tcacgacgac
     4801 cgacgctgat gttgtgataa cattatattc tttcataagc cgttccttta atggtgaata
     4861 aaacgaacgg tgcattttat acgtgttcga tcatgtccaa aatctggagc cgtgtggatt
     4921 ccaggcgctg ggtcacgata tgcgaaaacc gtttgagcaa ttggtatccg agttcgtgat
     4981 cagattcgca tttgttgcgc agacatcgtc cgtccagcgc tatagctaat gtcagatcca
     5041 aagcccgcgc atcaaattgc caatggtacg gcgggatgag ccatgaccat ccgaggatat
     5101 cgccttcggt caacgtatgt atgccgatgt gaccgcgacc gggcgatccg atttctaatg
     5161 caatcttgcc ttggcggatc agataaaatt cgttagcctc ctgcccttca cgaaaaataa
     5221 tttgccccgt atcaaaccgc acattggagg cgcagccgat gatcaattca acatgttcgg
     5281 ctgacatatc aatcagtaac gggtgctgca tcagaaaaaa cttcaaatct ttcatggtgg
     5341 ttttccgtct taaaaataag cgccggcata acaggcacac ccggacatca aacgatacag
     5401 ccgtgagctg tatgtaaacg aatcgtacac gtactcgggt cctggatatc gggggaaggc
     5461 gggaacgtta tgccggcaaa agttttcata caccggactc ctatagtcct gtaacttaag
     5521 ataaggctaa tgactcgact tcgcgacgac gtaggtcatc gcgatgtgtg atatgcgtca
     5581 gcggtttaag tgaaatgact ctaagttgtt gagatttata gaagatcaaa gtacgagagg
     5641 ggagggggta ctcagggttg aagtttaata cgttcctttt tagggagttt tttgacgact
     5701 tcgtcgtagg aatgatcgat catcagaaaa atttccttat cctgcacgga tccgtccaaa
     5761 tacacgctat tccacaattt tttattcatg tggtaccccg gttgaacgcc ttcgtaacgc
     5821 tcgcgccatt cgatggcttt ttcgggatcg gctttgagat tgataaattc ggcaatatga
     5881 gtattgatga gagcaaaaat ttttccgctg acccggaata ccaatgtttc ctgatcaaaa
     5941 ggaaaatctt catcggttga ttttttctta agacaatatg tgcggatcgt ttcgagattc
     6001 atgatgtgtg cgtaatttta gtttgaattc acttcgttaa aaaaacagaa ccccgctttt
     6061 cagcggggct caacgatttc cgggaacccc cgtagcacgt cgctagcggg catcaccgga
     6121 ggttcccatt aagttatgct taatacggat cacctcctat ccccagggtt gaaacttctc
     6181 gcctgaaatc gccctgaaca atttcacgcg aagcgttaat tacgtattaa acttacaagc
     6241 tatcctttcc attgtcaata ctcattttgg cagataacac tcctgttgaa atgttgttct
     6301 gaaaacatct gcaaatacgg ttttcaaaaa acagttcttg ccaaggcgct aagttttaaa
     6361 ttacaatcga agcatttttt atctttgcga cttcgcgagg gaattttatt gatggtagcg
     6421 tcggtaagta cacgacgcct cggtacgttg cttcgttcct actcggtgtc agaaggtgta
     6481 ttaatactat agccggatat aagcgcttga tggagaaatg atttgtgaag cggtgtcctg
     6541 cgtgagggat gcggagggtg agctcgcgta cgggcgcgag cgaagacccc gtaaacgcgc
     6601 caaaggcgcg tgcggaggcc gatagcccgg agcaagcccg acccgccacg ccgtaggcgt
     6661 gacggggcac gcccaaaaat taaatatcta taatttatta tgcaccggct tcgtcgcgta
     6721 aaccgaaacg catcggatgc ggcggaagat tgatgatttc atccggataa aacgatacga
     6781 tataatcaat cacgccgcgg acagatgtaa acgcaaccgg ttcgccgttt tcattgacga
     6841 tcgggatatg gcgtacatcg tgcagtgcaa ggatacgaat cgccgcgctt acagggtcgt
     6901 gcatttgcaa acactgcggc gcgacacgca tatagtcgtc cactttagca gtatcaaaat
     6961 ccggcgtctc gtgtgcgatc ttgtataata aaaaacgctc cgtcacggta ccggtcaatt
     7021 ttttgtcacg tacgaccatg acgcaaccga tattgcgatc ggccatctgc cgcaccgcat
     7081 ctttgagcag gctgccggac tccacacaca aagccgcggg cggatccagg acaccgatcg
     7141 gacggtcaaa atcttcggga tcaatgcctt gcttttcgcg ggcccgtttt ttttcgggtg
     7201 aacgcggttt tttttcttcg gtatcaatcc ggacatgcat tttggccaaa atgtgatcca
     7261 agatgtcttc gaacgtgaga atgccgctcg gatcgccgac ctggctgagt acggtgatat
     7321 tgcgaaatcc gccgaacgtc atttcattga gcgcgtactc cgccgaatcg tcggcataca
     7381 aacaatgcgt atcggcgctc atcaggcgcg taacgggcgt atgtgaaaat tccatatgca
     7441 ttgcgatcag attgcgaaga atggtccgct cggacagcgt cccgatcaat gcgcctttgt
     7501 cacgcaccaa aatacatccg cttttggttt gctccatcat cgcgaccaca tcggccacgg
     7561 tcgcatcttt ttctacatag agcggtttgc ggggattgag tgaatccaca cgggctttgg
     7621 taattcctgc attcatggta acctcgccgt taaaatgtcc gttgaacgga ccgttgatct
     7681 atcgtttttt ccacttctcg catccatttt ttccgatcat gcgccccacg tcatgcattg
     7741 aaaccggtcg gtatgatcgg cgagaatttt ctttttatct tcatccgtac taaaaaacaa
     7801 acccgtagcc agcgcatccg tgtcgcgtcc attggtcccg atgatagtca ggctttggaa
     7861 ctgcgcccgt ttgggttcgg ctaatttggg atcgatcaaa tgatgataca cgcgtccgcc
     7921 ggaacggaaa tactgttcat acgtacccga cgtcgcgatc gcctgatcgt gcagtgtcac
     7981 gactgtcgcc atcacatcgg cacgttcggg atcgcgcaca ccgacgcgcc agccttcgcc
     8041 gtcggcacgc cggcccagtg cgacgatatc tccgcccaga ttgatcagcg cctgcgtgat
     8101 cccttctttt ttcaaaactt cggcggcacg atcggcggca taccctttgg cgataccgcc
     8161 aagatcaagc cgtacgtcgg gtgataaaat ttttaccgtc gtgtcggagc ctgtcctcac
     8221 atgaataaaa cgatgcaggg aaagtccggc gaaccgctgc cacacgtctt gcgcgggcgg
     8281 ctccgtgcga tgtttcacat cccaggtatc gctcactttt cccagtccgg gctcaaatcc
     8341 gccgtcggtc accttggacc aatgcagcgc ccgttcgatc agatcggctg tttccgatgc
     8401 gacgcgaatt tccgcgcccg tcgtagcggc attgatacgt ccgacatcgc tgaccggtga
     8461 aaaatatgtc aataaacttt caagtctctg caattcatca aacgcggctt ccatggcgcg
     8521 atcggcccaa cgcgcatcgt ccgacacgac atggatttcg ccgatcgttc ccatcagagg
     8581 gcgcgtcatc gtgtacacac gacgtcttga taaaagcaat ggtcccagac cggctccggc
     8641 acataccagc agcatggcgc cgcttgtttt gagaaattgt cttcggtgca taaaaaattc
     8701 tccgttctta tttcgtcggt gcagccgatc cggcccacgg catttttttg cgaagggttt
     8761 cccgcgcttc gcaatgatcg ccgccgcagc ttccgtcttc atcgcagtga ccgcacgacg
     8821 gcttatggtc aaaaagattt tttgttttca ttcggcgtgc gatcatctga acgataatcc
     8881 aaaacgcgca caaaaatgcc gatgcaaaaa cgggcaggat gtatttggct aattccataa
     8941 ttttaattgg agctgaataa tccggcgaaa cccataaacg ccatggataa aataccggca
     9001 ataatcaaac tgagcgccgt accctgcagt gttttaggaa tatccatgat gcgtgtttcc
     9061 tcacgaatgc cggccataat caccagagcg agcgtcatgc cgccgccgcc gccgagcgca
     9121 taaaacaacg cttgcaccaa tccgtattct ttggacgtct ggaataacgc aatgccgaga
     9181 atcgcacaat tggtcgtgat cagaggaaga tagataccga gcgcacgaaa aagttccggg
     9241 cttagttttt tgataaccat ttccaaaaac tgcacggtgc tggcaatcac cacgataaag
     9301 ctgatgagcc gcaaatacgg cgcgtacaca agtacatagg tattaagtgc ccatgcacaa
     9361 atcgccgtaa tgagcattac aaaaatattg gctaaaccca tacggaatgc tgtttcaatt
     9421 ttattagaca cgccgagaaa cgggcaaatg ccgagaaaat tggccaacgt aaaattattg
     9481 atcagcagcg ccgaaataaa tatccagagg tattcgccca tatacgccta cactttcgcc
     9541 gtttgacggc cgaacttgat ccaattgatc acaaccaaca atgagccgag caataaaaat
     9601 ccgcccggcg gcaatatcat gataacccac ggttcgaacg cgtcaccgaa gagtgatacg
     9661 ccaaaaagag aaccattgcc taaaacttca cggatgcatg aaagcgtcat caatgcgccg
     9721 agaaacccga gtcccatacc gagtgcatcc atcacggcaa gccacggcgg atttttgtat
     9781 gaaaaagctt cctgccgccc gagtatgatg cagttaacca cgatcaaggc gatgaacgcg
     9841 ccgagttctt tatgaatttt aggaaaaaaa gcttcgagcg attgatcaac cgccgtcaca
     9901 aatgtggcga tgatcacaat aaaagtcgtg atgcgcacct gtttgggtat ccaatgacgc
     9961 agcatggaca ccaaaaggca tgagcctaca agaacaaatg tggtggccag tcccatggcc
    10021 aatccattga tcgccgaatt ggtgacggcc agagtagggc acatgcccag gacggcaaca
    10081 aatgtcggat tttctttcca caagcctttg atcaattcgt gtgttgcgcg tccgcttttt
    10141 tcaatcgcgt tcatggttgt ttctgattat aagtaagatt gttctgctgc atgtaaccat
    10201 caatggcgac ttgccatcgc gtcatggctt tttgtaaagc acgaataaca gctttcgacg
    10261 atatggtcgc accggtgatc gtttcgacct gattgggttt ggtttttttt ccggtctttg
    10321 cggcctcgat ctccggttcg accgacagcg ctttaaaatt agcaacgaaa tttttgtctt
    10381 tgatgatttt atcgccgagc ccgggcgttt ctttattttc gagcacttca aatccgacga
    10441 tgatttttcc gtcggcctga tacccgtaaa tgattttgat cacatccgca aaaccgggct
    10501 ccgatgcagg aatcgcaaaa ccgacgaccg attttttttc atcgtacccg agaaatatac
    10561 gatcgccttt ttttgtttct tcgccctgcg ctttttcaag gcggtcgttt tttaaaacca
    10621 atacttcata actgcggcaa cccggtacga cgcgatagat ggcttcctgc aacgcttctt
    10681 ctttattctg ctcgatgatc ggtagcgtcg aaaaataagc gaccactaaa atcagccctg
    10741 aaaaaaaacc ggccacagcc aacgtcagga tcaaaatcaa tgagctgggt tcggcttctt
    10801 tcgtcgcaac gggtgttgcg ggtatatgtt gatgttccat aatttatttt atttcgttcc
    10861 gtaaacacga tggcgcgtaa cgcggttgat caacggcgtt acggcattca tcagcaatat
    10921 ggcatacatg acgccttccg gcaaaccgcc gaagatgcgt atgagcacga ctaaaattcc
    10981 tatcccgctt ccgaaaatcc acatgccgcg caccgtcatg ggcgacgtca caggatcggt
    11041 ggccataaat acggcgccca gcatcaaacc gccggagaaa agcatgtgca tcggtgaagg
    11101 atataaaatc gggttgacga tatacaaaat gcccgacagt acgacggtag cagctatgat
    11161 agaaaccggg atgtgccagt tgaaaatttt tcgataggcc agatatacgc cggctaaaag
    11221 tataatcaca ccgcaggttt cgccaagcga cccggaggta tggcccagca taagatgtga
    11281 taattcggtg gcttcatgct gaaacttcat actggaaagc ggcgtggcgc tggagattgc
    11341 gtctaacggt tcgccgtgaa aaaaaggcca tgccagatta gtaccccgtg cggaaaaata
    11401 tcgtccgtcc ggtaccgacc acgtcgtgat cgccgtgggg aatgcggcct gcagaaaagc
    11461 acggcctacc aatgccggat taaaaatatt ttgtccgagc ccgccccata tatatttacc
    11521 gatcatgatc gcgatcagtc caccgagaaa tgccatccac atcggaaaac ccggtggcaa
    11581 agtcaatgcc agcaaaagcc cggtcaataa agcgctgccg tcgaagagtg attggtgacg
    11641 cggattcgat ccggacaaaa gccattcggc aaacaaacat ccgctcacgg cggcaaccga
    11701 cagtaaaatc acactcagtc cgaaaaaata atacgcggct gccgctaccg gcactaacgc
    11761 gtaaatcact tgacgcatga tccaaggcgt cgtcgcttcg tcatgcaaaa acggcgacgt
    11821 ggacagtgtt aatttggatt gggtttcgat gacgctttcg cctttctttc acgtatagca
    11881 tttttagcaa cccgaaaact ctgtactaaa ggaattcccg aagggcagac aaacgaacac
    11941 gacgcgcatt caaaacaatc catcacgtgc tgttcttcca gttcatcata caaatctttg
    12001 cgtgagagca gacccatgag ggaaggattg agaaacatcg ggcacacatc tatacagcgc
    12061 ccgcacttga tacattggta tgtggaaagt tcctgcacct cgttatccgt caggacgagt
    12121 acgcccgaaa tacctttggt tacgggtaca tccaatgttt tttgtgcgat gcccatcatc
    12181 ggcccgccga gcaaaattcg aacggcatct tgcgtaacgc cgccgcacca atcaatcaga
    12241 tggcgaatcg gcgtaccgat cggcaccatt aggttagccg gtttgcgaat agccgggccg
    12301 gtcacggtca ctacgcgttc gatcaacggt acggcgtttt caaaatattg cgccaaggcc
    12361 accaacgtac cgacattgga cacgagtgcg ccgacatcca gcggtaattt acccgacggc
    12421 acttccgttc ccagtaccgc tttgatcaac attttttctg caccttgtgg atatttgacc
    12481 cggagcgcta ccacttcgta atcgcaattt tgttttttga tttcttcctg caacgcggcg
    12541 atcgcatccg gtttgttatc ttccacgccg atcacgaccc gctgtgcggg aacaaaatgt
    12601 ctgagtatcg tcaggccatg aaccaagtcg gccgcaaact ctaccatgat gcgatgatcg
    12661 gacgtaagaa acggttcgca ttcgcagcca ttggcaatga tcgtcgtaca ggtttttcct
    12721 tcggggatgg aaaacttcac atgcgcggga aatccggcgc cgccaaggcc taccaaaccg
    12781 gcgctctgta cagcggcgat aaattctttg tgtcccatat ttttccaatc caacggcggt
    12841 cgctgcggta cggtctgtgc gctgtacgga tcggctttga tacgaataga gtacaccagc
    12901 aacccgttgg gatgttcgcc aagttcgata cccgtgacgg tacccgtgac gggcgcgtgc
    12961 tgcgctacgg atacgaaagc atccggtctc gcgatcacat cgccgcgtgt aacgtgttgg
    13021 cctttctgta caagcggcac cgacggtgcg cccgcatgct gcgcaaccgg aacggcgtat
    13081 tcttcaacga agggcatgcg ttcgatcgca agatcggcag tctgatcttt ataagcttcg
    13141 ggatgaatcc cgtggcgaaa cgtcaatcgt gacgacatgt tttccattat tgttttccgc
    13201 ttgcttgata tccggaaagc gctaataact tatcccgcaa cttgatgcgc gtggattcca
    13261 gatgatttga ttcctgttgt ttcagtttgc tctcatattc actacgcagt gtatcaagcg
    13321 ccgaattatg tttttcagac cattcggcat cccaatgcgc acgtaatttg accgggtggg
    13381 ccgaaagaag accggccatt tcgcgaagtt gtaaccacgc atcatagacg accgcacacg
    13441 tttcagccaa gacgcccgat agtaaataga acacgccttc gcgttcataa tacgcggtat
    13501 gtttagcacg ctcttcagcg gaagccaacc atgtacgaat atccaaactt ccggaagagg
    13561 gtttgcttac cgctgaaaaa tgcgatttcc acatcggctg aaaccaggcc cagtcggcaa
    13621 aggtcacgtt ttttccggtt acaaaatccg attgataatc gggattgtga tcaaggtgta
    13681 ataattcagc cgctaatttt ttcttcgcgt cggggatgta gcgcaacaaa ggaaatacac
    13741 gggattctac ggacatggcg tacatgtctt gcagtttgaa tttttctatc atcatcggcg
    13801 tatgcacgca catcatggcc ggcgtttggc tgcgcagaca agctgtaata ctgcggtaca
    13861 tgtcggccgg atagccgggt gatacatgag cccacgctgt ttgacgttgc gcaaaaagca
    13921 tggcattcaa tcccatacga tcgtacatat tttgtttgcc accggtggcg ggctcagtga
    13981 cgatccattt gatcggcagg ccgctattca taagcgcgat gatgtcaccc gccggcatgg
    14041 catcccgtac ggtcgcatcc gtcactaaaa gcaaaggcgg tgcaagattt ttttcttctt
    14101 ctgtaagatc attccaagtg atggccgcga tttgtatatc gtgttgtgac ggtaaatacg
    14161 cgttttgtaa ctcgagatcg gcacgccgta aaagtttgat attatcaatg atgtggcgca
    14221 tttgcccgag gccgataccg cggacgaagt ccggagcgat ggcttctgaa aaaaccatga
    14281 ccggcacgag gaacggatta tccggaaatt gacgtgccca tgccagagta tcattgccgc
    14341 tgattaccgc cgaataccgc gcacgtccga tacctgtagg tcctttcgtc aaaagccaat
    14401 gcagttgctt caagtcgcgc agaagatata cgacgcgttc gatccacgcc gtatcgacga
    14461 cgccaaatcg ttgcgaggaa ccgagttttc cgatcaaggc atcaagcgac atgcgttccg
    14521 tgccgtgttc ggaaacggcg gccaacaaat gttcggtact ttgtgtcggt aaagcgtcgc
    14581 gcaaaatatt ttgtaattta tccgtaagat tttggatacg cttttctatt gcagacgtgt
    14641 gcgtaagcat gcgtgcctgt gtggccgctt cgacagcggc cgtgatcaga tgcaacgcga
    14701 ttttttccgc cggttgatcc gtgcgtcggc tgccgagttg tgccatataa taatgacggc
    14761 tcagaaataa ggcggccagc gggttgtaac tttcgttatc taaatattgt ttgaccgtgg
    14821 ctccgggcgt atccggcagc gcttcccaaa tccggtacga cgcttgtaac tgcgcttcgg
    14881 acgccggatt caccggttgc atgcttaggg cttgttccga acacgatgca gcacaaatgc
    14941 cgcatccgct gcaagcatgc ggatctacgg ccaatgaaaa aatcaagccg ctgcctttag
    15001 catattgttc cggtgttttg aaaaatgttt tggtaatcac agccggaagc ggggcaagat
    15061 attgaccgac gtcggtcgct tgcgcgatca aattttcttt tttggatgaa tccattttca
    15121 tctgcacggc taatttttcg gcggccacag gaagcaggtc gcataacgat gggttgtttt
    15181 ctgataccat cggatgcaac agtttggcga gatttttgat taccggcgga gtcatttctg
    15241 tcagcgattt tccggcccgt atcgtttgct cgacagctga ttgtacgagc gcttccgtcg
    15301 aaagagctac catcggcaga gcggattcag ggcaatatac cgcacattga ccacaaccgg
    15361 tgcatgactc cgattttaac accggataca tcgcccgttg tgctgtggca ttgtgaaatg
    15421 ccgacgtcgc cgggggtatg accggtaagg catgaaacgg atcagccgtc atggctttag
    15481 tttgaccgga ctctgtaaaa aatccgacac gatcataaaa tgaagccaca cgcgaatacg
    15541 gtgcgccacg atcttcgtag cggcgtacga taccgggaat gtcagacgaa atcgcagtca
    15601 ttgttttggc cgattcatcg agtcctatcg aacgcgcatt caaatccgga aattcgcgat
    15661 gcaggttttg aaaatgaatt tgttgtttcg gatacgacga cttg
//
LOCUS       DAOQGF010000078        15541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1745_length_15541_cov_6.475526, whole genome shotgun sequence.
ACCESSION   DAOQGF010000078 DAOQGF010000000
VERSION     DAOQGF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_15541_cov_6.475526"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..500)
                     /locus_tag="PK937_10930"
     CDS             complement(183..500)
                     /locus_tag="PK937_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33128.1"
                     /translation="MNIQYDEIEISKPFVNEPGLDDDRTLILQTNNARRWLHKLIDEM
                     TLEQRFLLLDYAQSLITHQPHEETSSTLDSKINVLRAIEEAAKKRNEEPLANLISDIM
                     RPH"
     gene            complement(509..1351)
                     /locus_tag="PK937_10935"
     CDS             complement(509..1351)
                     /locus_tag="PK937_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNH33129.1"
                     /translation="MMHRITPYLTIIRPLNALISFLSPMLILVISDASEDEVAWGIAL
                     LSGIVAAFVCAGANVINDIFDLEIDRINKPHRVIASGAMSLMAAKRYYVFLTGSAVIL
                     SAYICWPVFGIVVFSVLLLYYYSVYLKRTPIWGNLTVCFFTSVNFLAGGMAFDQTEHA
                     WYPALFTFLFQFGRELLKDVEDIEGDRVGGANTFPVMYGVRASLLIVTLSFIILSIAM
                     YWPYHTGFYGKWYALIVMIGLMPLIVAVTFMMWRDTSALKIRRLNQLLKLAMFIGLFA
                     IYNG"
     gene            complement(1441..1815)
                     /gene="rplS"
                     /locus_tag="PK937_10940"
     CDS             complement(1441..1815)
                     /gene="rplS"
                     /locus_tag="PK937_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNH33130.1"
                     /translation="MNKVDAFTAFQLRNDIPEFRAGDTVAVDVKVVEGDKERIQTFEG
                     VVIQRRGSGIHESFTVRKISNGVGVERIFLANSPRLGTVEVLKRGRVRRAKLYYIRGR
                     RGKSARIQDIVDTTETAQSAAK"
     gene            complement(1844..2587)
                     /gene="trmD"
                     /locus_tag="PK937_10945"
     CDS             complement(1844..2587)
                     /gene="trmD"
                     /locus_tag="PK937_10945"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNH33131.1"
                     /translation="MSTLRIDIITAFPKMIDDALGHSIVGRAAKKGLVQIRAIDLRTY
                     TQDRHRTIDDTPYGGGGGMLIKPEPMFACIEDVLQIPPITSANHVRDVISPDTDVIMT
                     SPMGQTFDQKKAVEFSLKKHLVFICGHYKGIDERVHEKLVTQSVSIGDYVLTGGEIPV
                     LAMVDATVRLLPGAIGDAQSALTDSFQEGVLDCPYYTRPENFRGMKVPEVLLQGHHEA
                     IQTWREEQRLAMTRKLRPDLIRKEEVLKK"
     gene            complement(2609..3136)
                     /gene="rimM"
                     /locus_tag="PK937_10950"
     CDS             complement(2609..3136)
                     /gene="rimM"
                     /locus_tag="PK937_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNH33132.1"
                     /translation="MQSEALIQVGRILRSHGLRGELKIEVFNDGLEELESGTPCVLKS
                     RFGKQAEMHIERIRFGNDTILKLREMQDKSSADVWSQGEIWLLEKLLPALPEDAFYVK
                     DITGLEVRDESGKKIGILADVETSPAHDLYVIEVQGKRYRIPAVAEFIKAINKETGVI
                     TIHLIPGLLEYQTAS"
     gene            complement(3143..3373)
                     /locus_tag="PK937_10955"
     CDS             complement(3143..3373)
                     /locus_tag="PK937_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNH33133.1"
                     /translation="MQSFIEFVVKHLVDKPDEVEVKEVDGERVVVYELKVGQGDLGKV
                     IGKRGQNAKALRTLLAAASARAGKRAVLEILE"
     gene            complement(3419..3820)
                     /gene="rpsP"
                     /locus_tag="PK937_10960"
     CDS             complement(3419..3820)
                     /gene="rpsP"
                     /locus_tag="PK937_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNH33134.1"
                     /translation="MVKIRMQRGGKAHQPFYRIVAAHSTRKRDGDYIERIGSYNPLTT
                     PPQITIDEKKALKWLTDGAQPSDTVKTLFRSAGILHRWRLTAKGLSVEEVNAEMEKWK
                     ANQAKKAAEKRAKRTKAVKKKTEKASEGSAA"
     gene            complement(3958..5643)
                     /locus_tag="PK937_10965"
     CDS             complement(3958..5643)
                     /locus_tag="PK937_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33135.1"
                     /translation="MKTWIILLSFTAIANAQNSELYFQSDSERALFKDSIFFKTNNST
                     EVFENLLITEGVSDSSSIRKYRGYLEKIAARLSKEINLKSKASKKAEVIFTYLHKNIF
                     KKYNIDTRFQDLFSRGEYNCVTATYLYYDMCKRFDLNVTFYASAVHIYPVVKGEQRPI
                     NVELTDPVNGFDFREDRKAIINYLLEYKLITQDELKSKGSDAVYREYVDKTKEVGIPA
                     LYSISYSNQGISYWQAGKLKETLWAMEKALSIIWDDSKSRAIYQSVFDENLNKILYDV
                     PSAYPVVIRGLQFMRSDTSFVRDHFNFIMHAIEYYSVTKLEYDKALEIVNEGKAIFSF
                     DTSLVKRLTEFSRDVRWNQFAYVYNASNYEKAAAILEIGLSEFPNDVRFLDFQKKTDL
                     LLAERLSDKGDYQGAINRLSGLRKKYSSDFRIEDTYVGTVTYFASKCAQNGESKKAID
                     LMDSLASSLPENFKVKESYSAIVGLIISTEELDKKNIYEARRLIIKAIQVDTKNITMT
                     KMLAAIYHELAMAKVRKKEYLAAKNLILEGLGYIPQSYELNDDLKLIKQQLGK"
     gene            complement(5695..7032)
                     /gene="ffh"
                     /locus_tag="PK937_10970"
     CDS             complement(5695..7032)
                     /gene="ffh"
                     /locus_tag="PK937_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNH33136.1"
                     /translation="MFNDLSAKIEGAFKRLAGRGVINEKNIQEALREIRMALLEADVN
                     FKVVKDFIDKATAKALGQEVLRSVSPGDQLVKIVYDELVETLGHEHAPLKHAGIPPTV
                     VMVVGLQGSGKTTFSGKLANYLRKKGRKPMLVAADVYRPAAADQLRTLGKTLDIPTFS
                     PDEAGSHDPVKICFDAVTYARQHGRDLIVLDTAGRLHIDDALMTELEQIKIKTQPNEI
                     LFVADGMTGQDAVNTAAEFVKRLDFDGVVLTKLDGDARGGAALSIRSITGKPIKFIGT
                     GEKMDALEEFYPDRMASRILGMGDIVSLVEKAQETIDQEQAQKMHEKMMKNEFTLADF
                     STQLGQIKKLGSLESILSMIPGVGSKIKGMQMDDKVLVRMEAIISSMTPKERERPQML
                     DGRRRLRIARGSGTSVQEVNRLLKQFDDMKRMMKKIGKMNPAEVMNMMSQMGR"
     gene            complement(7055..8878)
                     /gene="typA"
                     /locus_tag="PK937_10975"
     CDS             complement(7055..8878)
                     /gene="typA"
                     /locus_tag="PK937_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNH33137.1"
                     /translation="MKRRTDIRNVAIIAHVDHGKTTLVDQLLRQSGTFRENQQIAERV
                     MDSNDLERERGITILAKNTSIRYKGAKINIVDTPGHADFGGEVERIMTMVDGVLLLVD
                     AFDGPMPQTKFVLKKALASNCTPIVVINKIDRPGARPHEVLDEVFNLFLELNANDEQL
                     DFATIYASAKMGFAQYEIDQANSDMVPLFDTILERIPSPPGEREAAFQMLVANIDYSD
                     YLGRIAIGRIQRGEIKTGQNVAVMKKDGTRYNSRVVKLFAYEGLKRTETESAYSGEIV
                     AMAGMEEIQIGETIADYNNPEALEPIHVDEPTIAMNFIVNNSPFAGQDGKFVTSRHLR
                     ERLYKELLSNLALRVEDTESMDTFKVSGRGELHLCILIENMRREGFEFQVSRPEVIMK
                     RVDGQLLEPMEYLIIDVPEEYMGVVIEKLGKRKAEMKNLIHLDNKQVKIEFVIPARGL
                     IGYRGEFLTDTRGTGVLNHIFHDYAPYKGDIGGRLNGVLISMEQTETVAYALFGLQER
                     GELIVDPQTPVYEGMIIGKHSRENDLVVNVGKGKKLTNMRASGSDDAVRLEPVRPMSL
                     EDYIEFINDDELVEITPKTFRLRKKVLKESDRRREERKKKD"
     gene            9376..10122
                     /locus_tag="PK937_10980"
     CDS             9376..10122
                     /locus_tag="PK937_10980"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNH33138.1"
                     /translation="MEIAIFFAGHWFTSLFSQSFFLHRYSAHKMFNMNAFWERFFYFF
                     TFLTQGSSFLNPRAYAVMHRMHHAFSDTEKDPHSPHFFKDVFRLMWHTKKVYFDLLTG
                     KTIPEQQFDGDTPRWGWLDAMSNAWVVRVAWGVGYFIFYYFFVPEGYTWLYALLPLHF
                     LMGPIHGAIVNWCGHKYGYANFDNRDKSKNSLVWDFMLLGELFQNNHHKYPNRPNFAV
                     RWFELDPTYPIMKVLDWMRIIRIRRKSNAA"
     gene            10133..10483
                     /locus_tag="PK937_10985"
     CDS             10133..10483
                     /locus_tag="PK937_10985"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNH33139.1"
                     /translation="MLTFLKILFSVIFAGMIAVVVTTSYESNLFTLIATWDSANTMAP
                     WFSATLWDFYANVVFIFVWIAYKENNVFRSIVWLILLVALGSIATALYALIQLFKLQP
                     GQNIRDFFTAQNAA"
     gene            10499..11278
                     /locus_tag="PK937_10990"
     CDS             10499..11278
                     /locus_tag="PK937_10990"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNH33140.1"
                     /translation="MVHDFWNYQLIALIVMILIMTIVWIIHLIIRRADIVDIAWAYSL
                     AILAGMFALLSPYEGLRPVLMAALVGVWGFRLGTHLLLRIAGHEEDGRYQQMRREGKG
                     ALEFFWFFQFQALLNVILCIPFSFVALNPVNRLGFFEYAGLSIGVIAIIGESFADAQL
                     RRFKARTDTRGQVCRDGLWKYSRHPNYFFEWLIWVAIFVFACGSPLGWISVIGPLLML
                     YFLFKVTGIPATEAQARRSKGLAYAEYQRTTSAFVPWVNKQ"
     gene            11300..12328
                     /locus_tag="PK937_10995"
     CDS             11300..12328
                     /locus_tag="PK937_10995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNH33141.1"
                     /translation="MIHTLLKNNLIPDAFIRYGIRRLLRQRLFEEKKPTIELQWQHWK
                     NYINMLKESPIAIDTKAANEQHYEVPTRFFQLVLGRHLKYSSGYWHEGVTELDRAEND
                     MLALTCSRADLHDGQDILELGCGWGSLSLYMAEHFPQSRITAVSNSRTQKEFIDATAR
                     QRGFSNLTIITADMNHFTTDQKFDRVVSVEMFEHMRNYRALLAKVARFMKDDARLFVH
                     IFTHREYAYLFEVRDESDWMAKYFFTGGQMPGDMLLHYFNEDLRIEEHWRVSGLHYAK
                     TSEAWLANMDRQRAEIIPLLEATYGTNNARMWWVYWRIFFMACAELWQYNQGDEWLVS
                     HYRFRKNI"
     gene            12325..13050
                     /locus_tag="PK937_11000"
     CDS             12325..13050
                     /locus_tag="PK937_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33142.1"
                     /translation="MNLKRSYYLLLTLSSVSLSAIVSPTETPTLQLKTLAWDNKSGAL
                     LYTEERKQFDFDGKPGRWEFNYQNPKGQIVVQRSVNFQQDRLKPSFILRDLRNGYSEG
                     AETIGNKIKVFSGGSPDDPYQEKTIRVPEPAVIDAGFHFFIEQHWSALLNGETKTFYF
                     VAPSQLDYFSFRVYKSGETQNGGRTAYQLTMDIDNFLLRLFVDPIRLTYDPESRKLIG
                     YEGISNIYNDEGKAHHVRMEFVY"
     gene            13074..14339
                     /locus_tag="PK937_11005"
     CDS             13074..14339
                     /locus_tag="PK937_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNH33143.1"
                     /translation="MERIAIIGTGIAGMGCAHFLNHRYDITMYEQNNYIGGHTHTITV
                     HEDGSPVFIDTGFMVFNLVTYPNLVRLFKELGVDYKPTSMSFSVQHVPSRLEFNGSGI
                     NELFAQRRNLLNPSYIRMLLEINRFNNTCREVIEKSQFADYTLKEYVEEKGYSDDFLM
                     KYLVPMSSAVWSTPPDAMLGFPAAALIRFFDNHGFLGLHTQHPWYTPVNGSQQYREKL
                     IAPFRDRIFTDRAAVHVRRENGKVFITDKRGDKAAFDKVIIATHADQALAMLEQPSEA
                     EYRLLKEFKYQKNITTLHTDASGMPRTRRAWASWNYRIDQKHDGSLEAHTIYWMNSLQ
                     HVSQKTNYFVSINGAERIAPDRVIRRMTYEHPVFSVAAMKAQSELPTLNIKEPVYFCG
                     SYFRYGFHEDAFMSALALCRQITGEAIWL"
     gene            14336..15139
                     /locus_tag="PK937_11010"
     CDS             14336..15139
                     /locus_tag="PK937_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNH33144.1"
                     /translation="MNSCLYRCRVMHHRMEPKPHRFFYNIFMFYLDLDELDTLSDKLT
                     LFGHNAFAVFNFKDTDHLFYSGTSRREHITTYLQQNGITSPVGRIMLLTHVRMLGYVF
                     NPVSFYFIFDNDEQPICAIPEVGNTFGEMKPYLIRHSTESPHTFYRKTTKFFYVSPFI
                     DIDAEFEFDLTLPDDQLMMRINDIQNNRRFFVSTLSGKRQILTDARLALYLLRFPLVT
                     LQVIFFIHWQAFRLWLKKLHYHKKTDFPDLQREVHHAGSYVNAIAAERR"
     gene            15096..>15541
                     /locus_tag="PK937_11015"
     CDS             15096..>15541
                     /locus_tag="PK937_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase"
                     /protein_id="HNH33145.1"
                     /translation="MQEVMSTPLPQSADNTKKIMPGFYEKLVLRTFEGMHQGYMRIHL
                     PDGRMYTYGNANAELQATIQVRSNNFFKKCALYGDIGFGEAYVDGDWDTDSVTRVIRW
                     FLLNVEQAPTISGSKRRIVPANFLKGINRLYHRLRENTVRLARMNI"
BASE COUNT         4157 a         3701 c         3218 g         4465 t
ORIGIN      
        1 tttgaggttc gggacattgc aaaaacatcc gcaccctcag ccaaccatgc tttcaccgct
       61 gccgcgccaa taccgccggt cgctccggtt acggccactt tgagaaccat actttacggt
      121 gatttattga tatgataaac taagtaaaac tacgcaatta tgcgcgcact agcaaccctt
      181 ttctaatgtg gacgcataat gtcactgatg agatttgcca aaggctcttc atttcttttt
      241 ttggccgctt cttcgatcgc acgcagtaca tttatttttg agtccagcgt agatgaggtt
      301 tcctcatgcg gctgatgggt tattagagac tgggcgtaat ccaataaaag aaatcgctgt
      361 tccagcgtca tttcgtcaat gagcttatgt agccatcggc gtgcattatt cgtttgcaaa
      421 atcaaagtcc ggtcgtcatc cagccccggt tcattcacaa aaggttttga aatttctatt
      481 tcatcatact gtatattcat tgtcctcatt atccgttgta tattgcaaat aatccgataa
      541 acatggctaa cttcagaagt tgattcagcc ggcgtatttt gagcgcggat gtatcacgcc
      601 acatcataaa agtcaccgca acgatcaaag gcatcaatcc gatcattaca atcagcgcat
      661 accacttacc ataaaaaccg gtatgatagg gccaatacat cgcaatgcta agaatgataa
      721 atgacaacgt cacgataagc aatgatgcgc gaacgccata cattacggga aatgtattcg
      781 caccgccgac acgatcacct tcgatatcct ctacgtcttt aagtaactca cgcccgaact
      841 gaaataagaa ggtaaataac gcaggatacc acgcatgttc cgtttgatca aaggccatac
      901 ctccggccaa aaagtttacc gatgtaaaaa aacaaacggt cagattaccc cagatcggtg
      961 tgcgcttaag atatacactg taatagtata atagcagaac agaaaaaacc acgataccaa
     1021 aaaccggcca gcatatgtaa gcgctcaaaa tcacggccga acccgttaaa aaaacataat
     1081 accgtttcgc agccatcaat gacatagccc cgctggctat cacgcggtgc ggtttattga
     1141 tacgatcaat ctcaagatca aaaatatcat tgatcacatt ggctcccgcg cagacaaagg
     1201 ccgccacaat tccggaaagc agcgcaatac cccatgcgac ttcatcttct gatgcatcgc
     1261 tgatcacgag gatcaacatc ggcgagagaa atgaaatcaa cgcattgagc ggtcgtataa
     1321 tagtcaaata aggagtgata cgatgcatca tgttaaatgt cgtaacaatg gcaatggaac
     1381 cgaataagga aaaaaaaagg gtatgccgat cgcataccct tattcaataa aagtcgaaac
     1441 ttattttgcg gccgattgtg ccgtttccgt tgtatccaca atatcctgaa tacgtgcact
     1501 cttaccacgg cgaccgcgga tataataaag tttggcccga cgaacacgac cacgcttcaa
     1561 aacttcgacg gtaccaaggc ggggcgaatt agccaaaaag atacgttcca cgccgacacc
     1621 gtttgaaatt ttgcgaacgg tgaatgattc atggatacca cttccacggc gttgaattac
     1681 gacgccttca aacgtctgaa tacgttcttt atcgccttca actactttta catctacagc
     1741 aaccgtatcg cctgcgcgaa attccggtat atcgttacgc aactggaatg ccgtaaaggc
     1801 atctactttg ttcatgggtt cctccaaaat atgtattgtt aaattatttt tttaatactt
     1861 cttctttacg tatcaaatcg ggacgaagct tacgcgtcat tgccaacctt tgctcttccc
     1921 gccatgtttg tatcgcttcg tgatgccctt gcagtaatac ctcaggtact ttcattccac
     1981 gaaagttttc cggccgcgta taatacgggc aatctaaaac accttcctga aacgaatccg
     2041 tcaacgccga ctgcgcatca ccgatggcac ccggtaaaag tcgaaccgtc gcatccacca
     2101 ttgccaaaac cggtatctcg ccgccggtca gaacataatc tccgatggaa accgattggg
     2161 taactagttt ttcatggacc ctttcgtcta tacctttgta atggccgcaa ataaaaacca
     2221 gatgtttttt caatgaaaat tccacggctt ttttctggtc aaatgtttgc cccatcggtg
     2281 aggtcataat tacatccgta tccggactaa tcacatcgcg tacatgattt gcactggtga
     2341 tcggaggaat ctgaagcaca tcctcaatac aagcaaacat cggctccggt ttgataagca
     2401 taccgccgcc gccgccgtaa ggtgtatcgt ctatggtgcg atgccgatct tgcgtgtatg
     2461 tacgcagatc aatggcacga atctgtacca gccctttttt ggctgcacgc cccacgatac
     2521 tgtgacccag tgcatcgtcg atcattttcg gaaatgccgt gatgatgtct atacgtaaag
     2581 tactcattgt aaaagaacat cgctccgttt aggaggccgt ctgatattcc agcaaacccg
     2641 gaatcagatg tattgtaata acgccggttt ctttattgat agccttaata aattcagcaa
     2701 cggccggaat tcggtatctt ttgccttgca cctcaatgac atacagatca tgtgcaggtg
     2761 aggtttcgac atcggctaaa attcctattt tttttccgga ttcgtcccgc acttccaaac
     2821 ccgtaatatc cttcacataa aaagcatctt cgggtaaagc cggcaacaac ttttcgagta
     2881 accatatttc gccttgcgac caaacatccg cggaagactt atcctgcatc tcacgcaatt
     2941 tcaggatcgt atcattacca aaacgtatgc gctcaatatg catttccgct tgtttaccga
     3001 accgcgactt caaaacacag ggcgtaccgg attccaattc ttccagaccg tcgttgaaaa
     3061 cttcgatctt aagttcgccg cgtaaaccgt ggctgcgtaa aatacgaccg acctgaatta
     3121 atgcctctga ttgcacgtac agttactcca ggatctccag cacagcgcgt ttaccggcgc
     3181 gtgccgaagc ggctgcgagc agagttcgta aggctttggc gttttgcccg cgtttgccga
     3241 tcactttgcc caggtcgcct tggcctacct tgagttcgta aacgactaca cgttcaccgt
     3301 ccacctcttt gacttcgact tcatcgggtt tatcaaccag gtgtttgacc acaaattcaa
     3361 tgaagctctg catcgtcatt cctccttaga agttagttgc agaccagtcc tgtatgaatt
     3421 aagccgcaga tccttcagaa gctttttcag tttttttctt caccgctttt gtacgtttgg
     3481 cacgtttttc cgcggctttt ttagcttgat tggctttcca cttttccatt tcggcgttga
     3541 cttcttcgac ggaaagacct ttcgccgtca ggcgccagcg gtgcagaata cccgcactgc
     3601 ggaataatgt ctttaccgta tccgaaggtt gtgcaccatc cgtaagccat ttcagcgctt
     3661 tcttctcgtc aatcgtgatt tgcggcggag tcgtcagcgg attataactg ccgatacgtt
     3721 cgatataatc accgtcgcgt ttacgcgtgc tgtgcgccgc tacaatacga taaaacggtt
     3781 gatgggcttt gcccccgcgc tgcattctga tcttaaccaa ggtattttac ctccaattcg
     3841 atgtacgtta attttatata taaactacta aactttttat acttaacaca ttttcacttt
     3901 aggcgcacat cataaaaaaa acaaaggatt aacgcaagat tttttttaat ctatacttta
     3961 cttgccaagt tgctgtttaa tgagctttaa atcgtcattc aattcataac tttgcggtat
     4021 atatcctagc ccttcaagta ttaaattttt ggccgccaaa tactctttct tacgaacctt
     4081 tgccatggct aattcatggt atatcgcagc gagcattttt gtcattgtta tattttttgt
     4141 gtcgacttgt atcgctttaa ttattaaccg tcgtgcttca taaatatttt ttttgtcaag
     4201 ttcttctgtt gatatgatta atccaactat agcggaatag gattccttta ccttgaaatt
     4261 ctctggcaaa gaagacgcaa gagaatccat caaatcaatg gcctttttac tttctccgtt
     4321 ttgcgcacat ttggaagcaa aatatgtaac tgtacccacg tatgtatctt cgattctaaa
     4381 atcggatgaa tatttcttcc ttaagcccga taaacgattt atagctccct gataatctcc
     4441 cttatcggac aaacgctctg ccagcaaaag gtctgttttt ttctggaaat ctaaaaaacg
     4501 cacatcgtta ggaaattcgc ttaaccctat ttcaagaatt gcggccgctt tttcataatt
     4561 gctagcattg taaacgtaag caaattgatt ccagcgaaca tcgcgagaaa actcggtcag
     4621 acgcttaacc aaagaagtgt caaaagaaaa aatcgccttc ccttcattta ctatttctaa
     4681 tgctttatca tattccaatt ttgtaactga ataatattcg atagcatgca taataaaatt
     4741 gaaatgatca cgcacaaaag aggtatccga gcgcatgaat tgaagacctc taattacaac
     4801 cggatacgcc gatggtacat catacagaat cttatttaga ttttcatcga atacagattg
     4861 ataaattgca cggcttttgg agtcatccca gattattgaa agggcctttt ccatcgccca
     4921 taatgtctct tttaatttgc cagcttgcca gtaagatata ccttgattgg agtacgaaat
     4981 ggaatacaac gcaggaatcc caacttcttt tgttttatct acgtattctc tatatacagc
     5041 gtctgagcct ttagatttta gttcatcttg agtaatcaat ttatactcaa gaagataatt
     5101 gataatagct ttgcgatctt cacgaaaatc aaaaccattt acaggatctg tcaattctac
     5161 atttatcgga cgctgttctc cctttactac cggataaatg tgaactgccg atgcgtaaaa
     5221 agtaacattt agatcgaaac gcttacacat atcataatac aaataagtcg ccgtaacaca
     5281 attgtactca ccgcgcgaaa agagatcctg aaatcgggta tcaatgttgt attttttaaa
     5341 aatattctta tggaggtatg taaaaataac ttctgccttt tttgaggcct tagatttcag
     5401 attaatttct ttggaaagac gggcggctat cttctccaag taccctcgat atttccgaat
     5461 cgaagaactg tctgatacgc cttcagttat cagtaaattt tcaaaaactt ccgtcgaatt
     5521 gttagttttg aaaaaaatac tatccttgaa caaggcccgt tccgagtcgc tttgaaaata
     5581 caattccgaa ttttgagcat tggcaatagc cgtaaagctc aataaaataa tccacgtttt
     5641 cattgatttc ccctcaccca tgttatacaa gagaatccga tcataaaacc aaccctatcg
     5701 ccccatttgc gacatcatat tcatcacttc agccggattc atttttccga tctttttcat
     5761 catgcgtttc atatcatcaa attgtttaag cagacggttc acttcctgta ccgaagtgcc
     5821 gctgccgcgg gcaatacgca aacggcggcg gccgtccagc atctgcggac gttcgcgttc
     5881 tttgggggtc atggaagaaa taatcgcctc catgcgaacc aaaactttat cgtccatctg
     5941 catgcctttg attttgctgc cgacacccgg tatcatcgaa agaatagatt ccagcgaacc
     6001 gagttttttg atctgcccga gttgcgtcga aaaatcggcg agcgtaaact cgtttttcat
     6061 catcttctcg tgcatcttct gggcctgctc ttgatcaatc gtctcttgcg ctttttccac
     6121 gagactgacg atgtcgccca tacccaaaat acgcgacgcc atgcgatccg gataaaattc
     6181 ttccagcgca tccatttttt cgccggtacc gataaatttg atcggttttc ccgtaatact
     6241 gcgtatagat aatgccgcac cgccgcgtgc atcaccgtcc aacttggtca gtactacacc
     6301 gtcaaaatcc aatcgtttga caaactcggc cgcggtatta acggcgtcct gtccggtcat
     6361 accgtcggcg acaaacaaaa tttcgttcgg ctgcgtttta attttgatct gttcgagctc
     6421 cgtcatcaat gcatcatcaa tatgcaaacg accggccgta tcgagcacga tcagatcgcg
     6481 cccgtgttgc cgtgcatagg tcaccgcatc aaaacagatt ttgaccggat catgggaacc
     6541 cgcttcgtcg ggagaaaatg tcggaatatc gagcgttttg ccgagggttc gcaactgatc
     6601 agccgcggcc ggacgatata cgtccgccgc caccagcatg ggcttacggc ctttttttct
     6661 cagatagttt gctaatttgc cggaaaacgt cgtcttaccg gaaccttgca aaccaacgac
     6721 catcactacc gtaggaggaa tgcctgcatg tttgagcggc gcgtgttcgt gcccgagcgt
     6781 ctcgaccaat tcatcataca cgatcttgac gagctgatca cccggcgaaa cgctgcgcaa
     6841 cacttcctgt ccgagcgctt tggccgtagc tttatcaata aagtctttga ctactttaaa
     6901 attgacgtcg gcttccaaaa gcgccatacg aatttcgcgc aacgcttcct ggatattctt
     6961 ttcattgata acgccgcgcc cggcaagacg cttgaaagcg ccttctattt tggccgaaag
     7021 atcattgaac atattttatc ctgtagtctg atccttaatc ttttttctta cgctcttcac
     7081 gacgacgatc cgattctttg agtacttttt tacgaagacg gaacgtttta ggcgtaatct
     7141 caaccaattc gtcatcatta atgaactcaa tataatcttc caaactcatc ggacgcaccg
     7201 gttccaaccg aaccgcatca tccgaaccgg aagcacgcat gttggtcagt ttttttcctt
     7261 ttccaacatt cacgacaaga tcgttttcac gggaatgttt accgatgatc attccttcgt
     7321 aaaccggtgt ctgaggatcc acgatcaatt cgccgcgttc ctgcaaacca aacaatgcat
     7381 aggctacggt ttccgtttgt tccatcgaga tcaagacacc gtttaagcgt ccgccgatat
     7441 cgcctttgta cggtgcgtaa tcgtgaaaaa tatgattaag tacgccggtt ccgcgtgtat
     7501 cggtgagaaa ttcaccgcga taaccaatca atccacgcgc cggaataaca aattcgattt
     7561 tcacctgttt attgtccaag tgaatcagat ttttcatctc ggctttacgc ttacccaatt
     7621 tttcgatcac aacacccata tactcttcgg gtacgtcaat gatcagatat tccatcggct
     7681 caagtaattg gccgtctacg cgtttcatga tcacttcggg acgcgacact tgaaattcaa
     7741 agccttcacg ccgcatattt tcgataagaa tacaaaggtg cagttcaccg cgtccggata
     7801 ctttgaacgt atccatactt tcggtatctt cgacgcgcaa tgcgagattg gacaataatt
     7861 ctttatacaa acgttcgcgc aaatggcgcg acgttacaaa ttttccgtct tgcccggcga
     7921 agggtgaatt attgacgata aaattcatcg caatcgtagg ttcatccaca tgtatcggtt
     7981 ccagcgcttc cggattattg taatccgcga tcgtttcacc gatctgaatt tcctccatac
     8041 ccgccatggc tacgatttca ccggagtagg cgctttccgt ttccgtgcgt ttcaggcctt
     8101 cgtaggcgaa aagtttcacc acacggctgt tgtagcgcgt gccgtctttt ttcataacgg
     8161 ccacattttg gcctgttttg atttcgccgc gctgaatgcg tccgatagca attcgcccga
     8221 gataatcgga atagtctata ttggctacga gcatttgaaa cgccgcttcc cgttcgcccg
     8281 gaggactcgg gatacgttcg aggatcgtat cgaacagcgg caccatgtcg ctattggcct
     8341 gatcaatctc atactgcgca aaccccatct tagccgacgc gtagatggtg gcgaagtcca
     8401 actgctcgtc gttggcgttg agttcgagaa aaagattaaa tacttcgtcg agcacttcgt
     8461 gcggccgtgc accgggacgg tcaatcttat tgatcacgac gatcggtgta cagttggaag
     8521 caagagcttt tttgagtaca aatttagttt gaggcatagg tccgtcaaac gcatccacta
     8581 acagcaacac gccatccacc atcgtcatga tacgttccac ttcaccgcca aagtcggcgt
     8641 gaccgggcgt atcaacgata ttgatttttg cacctttgta acgaatcgac gtgtttttag
     8701 ctaaaatggt gatgccgcgt tcacgttcga gatcgttgga gtccatcacg cgttcggcga
     8761 tctgttgatt ttcacgaaac gtgccgcttt gacgcaagag ctgatccacc aaggttgttt
     8821 tgccgtgatc aacgtgcgct atgatagcca cattgcgaat gtccgttcgg cgcttcatgg
     8881 caggggcact cccctttttt tcttcagaaa tcacatcttc gatcataaga ctccaggata
     8941 tttatcaaaa acaaaaacaa taaattacgt gttttattca aaggcgcggc aatatattta
     9001 tccgaccctg caaaaacaag tacttttata gcaaaatcca atcattacga atccaccgca
     9061 aaacttttaa attaggttag aatcttttta tccttttttt atttttatgt gctacactgt
     9121 agtatggcgc gcgcatttaa tacctgcacg taccgtggtg gcactcttaa gccacatata
     9181 aaattgttta ttcattaata aattaaataa aacaaataat tatatataaa atattacaca
     9241 acaactcaaa tatggctaat aaatgctaat aaaatagcac tattttctac ttgacaatag
     9301 ttttattttg tttaacattt gattgtccat attaaacatt ttaactctac tatttaaacg
     9361 cgaggaagat gtcgtatgga aattgcgatt ttttttgcag gacattggtt cacgtccttg
     9421 ttttcccaaa gtttcttttt acaccgttat tcggctcaca aaatgtttaa tatgaatgcg
     9481 ttttgggaaa ggttctttta ttttttcacg tttctgacgc aaggatcgtc gtttctcaat
     9541 ccgcgtgcat acgccgtgat gcatcgaatg caccacgctt tcagtgatac cgaaaaagat
     9601 ccgcattcac cgcatttttt taaggatgtg tttcgcctaa tgtggcatac caaaaaagtt
     9661 tattttgacc tgttgacggg aaaaactatt cccgaacaac aatttgacgg cgatacgccg
     9721 cgctggggtt ggctcgatgc gatgtccaat gcctgggtcg tgcgcgtcgc gtggggcgtc
     9781 ggatatttta tcttttatta ttttttcgtc ccggaaggat atacctggtt atacgccctt
     9841 ttacctctgc attttttgat gggtccgata cacggtgcga ttgtcaactg gtgcggacat
     9901 aagtacgggt atgccaattt tgacaatcgt gataaatcga aaaactcact cgtctgggac
     9961 tttatgttgt tgggcgaatt attccaaaat aatcatcata aataccctaa ccggcctaat
    10021 tttgccgtgc gttggttcga attggatccg acctatccga ttatgaaggt tctggattgg
    10081 atgcgaatca tacgtatacg ccgcaaatcc aacgccgcgt agaaagagaa ccatgcttac
    10141 ttttctgaaa atcctattca gtgttatttt tgcgggcatg atcgccgtcg tggttactac
    10201 cagctacgaa agtaacctgt ttactttgat cgctacctgg gactccgcga atactatggc
    10261 cccgtggttt tcggctacgc tgtgggattt ttatgctaat gtcgttttta tttttgtgtg
    10321 gattgcatat aaagaaaaca atgtttttcg cagtattgtg tggttgatct tgctggttgc
    10381 actcggaagc atcgccacgg cgctgtacgc attgattcag cttttcaaac tgcagcccgg
    10441 gcaaaatata cgtgattttt ttacggcaca aaacgcagca taaacgtaga aaagacgcat
    10501 ggtacatgat ttttggaact atcagctgat cgcgctgatc gtcatgattc taattatgac
    10561 gattgtttgg attatccatc tgatcatccg acgcgccgat attgttgata tcgcttgggc
    10621 atacagtctg gcgatattgg cgggaatgtt tgccttgtta tcaccgtacg aaggtttgcg
    10681 ccctgttctg atggccgcac ttgtcggggt ttggggtttt cgacttggta cgcacctctt
    10741 gttgcgtatc gcaggtcatg aggaagacgg ccgctatcaa caaatgcgtc gcgaaggaaa
    10801 aggagcattg gaattttttt ggttttttca atttcaagct ctgctcaatg tcatactctg
    10861 tattccgttt tcattcgttg cgctcaaccc cgtaaaccgc ctcggttttt ttgaatacgc
    10921 aggtttgagc atcggcgtta ttgcgattat cggcgaatcg ttcgcggatg cgcaactccg
    10981 tcgtttcaag gcacgtaccg acacacgcgg ccaagtctgc cgcgatggat tatggaagta
    11041 ttcacgccac cctaattatt ttttcgaatg gttgatatgg gtggccattt ttgtttttgc
    11101 gtgtggctca ccgctagggt ggatctccgt tatcggccct ctattgatgc tgtattttct
    11161 ttttaaagtc accggtatcc cggctacgga agcgcaagca cggcgctcca aaggcctcgc
    11221 ttatgcagag tatcagcgca ccaccagcgc gttcgtaccg tgggtcaata aacaataaca
    11281 aaatatacaa aactacgaca tgattcatac cctacttaaa aacaacctca tcccggatgc
    11341 gtttattcgt tacggcattc gcagactatt acgtcaacga ttgtttgaag aaaaaaaacc
    11401 gacaatcgaa cttcaatggc agcactggaa aaactatatc aatatgctca aagaaagccc
    11461 tatcgccatt gataccaagg ccgcgaatga gcaacattac gaggtgccaa cgcgtttttt
    11521 tcagctcgta ctgggacggc atctgaaata cagttccggt tattggcacg aaggcgtcac
    11581 ggaattggat cgcgccgaaa acgacatgct ggccttaact tgcagtcgag ccgatctgca
    11641 tgacggacag gatattttgg aactcggttg cggttggggt tccctatcgc tttatatggc
    11701 cgaacatttt cctcaaagcc gcatcaccgc ggtttccaat tcccgcacac aaaaggaatt
    11761 tattgatgct acagcacggc aacgcgggtt cagtaatctg accatcatca ctgccgacat
    11821 gaatcatttt acgaccgatc aaaaatttga tcgcgtggtg tctgtagaaa tgtttgaaca
    11881 catgcgcaat taccgcgcgc ttctcgccaa ggtcgcccgg tttatgaaag acgatgcccg
    11941 gcttttcgtg catatcttta ctcaccggga atatgcgtat cttttcgagg tacgcgatga
    12001 aagcgattgg atggccaaat atttttttac cggcggtcaa atgcccggtg atatgctttt
    12061 gcattatttc aatgaggacc ttcgtattga agaacattgg cgcgtatcgg gtttgcatta
    12121 tgccaaaaca tccgaagcgt ggctggccaa catggatcgc caacgcgcag aaatcatacc
    12181 gcttctagaa gctacttacg gcaccaataa tgcacgcatg tggtgggtgt actggcgaat
    12241 tttttttatg gcctgcgccg aactatggca gtacaaccaa ggcgacgaat ggctagtcag
    12301 tcattaccga ttcaggaaaa atatatgaac ctgaaacgat cctattacct tttgcttacg
    12361 ctttcgtcgg tcagtttgtc ggcgatagta tcgccgacgg aaacgccgac actgcaactt
    12421 aaaaccctgg catgggataa caaaagcggg gctctcttgt acacggagga acgtaaacaa
    12481 tttgacttcg acggaaaacc ggggcgatgg gaatttaatt accaaaatcc caaaggacaa
    12541 atcgtcgttc aacgttccgt aaattttcag caagacagac tcaagccttc ttttatactt
    12601 cgcgatttac gcaacggcta tagtgaaggc gcagaaacca tcggtaataa aatcaaagtt
    12661 tttagcggcg gttcgccgga cgatccgtat caggaaaaaa caatccgggt tcctgaaccg
    12721 gccgtgattg acgcaggatt ccattttttc atcgaacaac attggagcgc tttactgaac
    12781 ggtgaaacca aaacgtttta tttcgttgca ccgagccaat tggattattt ttcatttcgc
    12841 gtttataaat ccggtgaaac tcagaacgga ggccgcactg cgtatcagtt gactatggat
    12901 attgataatt ttttactccg ccttttcgtg gatccgatac gacttacata tgacccggaa
    12961 agccgtaaac tgatcggata cgagggtatt tccaatatct ataatgacga aggcaaagcc
    13021 catcatgtac gcatggaatt tgtttattaa tttcaattcg gagcgaagtt gatttggaac
    13081 ggatagctat tataggcaca ggcattgccg gaatgggatg tgctcatttt ttaaatcacc
    13141 ggtatgacat tacgatgtac gaacaaaaca actacatcgg agggcatact catacgatca
    13201 ccgtccatga agacggatcg cctgttttta ttgataccgg ctttatggtt tttaacttgg
    13261 ttacgtatcc caacctagtg cgacttttta aagaactggg cgtagactat aaacctactt
    13321 ccatgtcttt tagtgttcaa catgtaccca gccgattgga atttaacgga tcgggcatca
    13381 acgaattatt tgcgcaacgt agaaatttat taaacccgtc ttatattcgc atgcttttgg
    13441 aaataaaccg ttttaacaat acgtgccgtg aagtaatcga aaaatcgcaa tttgccgact
    13501 atacgctgaa agaatacgtt gaggaaaaag gatatagtga cgattttttg atgaagtatt
    13561 tggtaccaat gagttccgcc gtatggtcta ccccgccgga tgcgatgctt ggttttccgg
    13621 ccgcagcgct gatacgcttt tttgataacc acggttttct cggtttacac acccaacatc
    13681 catggtacac accggtcaac gggagtcaac aatatcggga aaagctcatc gcacctttta
    13741 gagatcgtat ttttacggat cgagccgccg tacacgttcg tcgcgagaat gggaaagttt
    13801 tcattacgga taaacgcggc gacaaagctg cttttgataa agtcattata gcaacgcacg
    13861 ccgaccaagc gctggccatg ttggagcaac cgtcggaagc cgaatatcgc ctattgaaag
    13921 aatttaaata ccaaaaaaat attacgacat tgcatacgga tgcctccggc atgccgcgaa
    13981 cccgccgggc ctgggcttca tggaattatc gaatagatca aaaacatgac ggttcacttg
    14041 aggcgcatac gatttattgg atgaattcgc tccagcatgt atcccaaaaa acaaattatt
    14101 ttgtttcgat caatggcgcg gaacgcattg caccggatcg cgtgattcgc cgcatgacct
    14161 atgaacaccc cgttttttca gtagccgcga tgaaagcgca atccgagctc ccgaccctca
    14221 acatcaaaga accggtgtat ttttgcggga gttattttcg ctatggattt catgaagacg
    14281 cgtttatgtc cgcattggca ttgtgtcgtc aaattacggg agaagcgatt tggttatgaa
    14341 ttcatgcctg taccgatgcc gtgtcatgca tcaccggatg gaaccgaagc cgcatcgctt
    14401 tttctataat attttcatgt tctatctgga tttggacgaa ttggacacac tatccgataa
    14461 acttacactt ttcggacata atgcgtttgc agttttcaat tttaaggaca ccgatcattt
    14521 attttatagc ggcacatcgc gacgtgagca tatcacaacc tatctgcaac aaaacggcat
    14581 tacttctccg gtaggtcgta tcatgttgct gacgcatgtt cgcatgttag gttacgtatt
    14641 caatcccgtg tcgttttatt ttattttcga taatgacgag caaccgattt gcgccattcc
    14701 ggaggtcgga aacacgtttg gcgaaatgaa accctacttg attcgccaca gcacggaatc
    14761 gccccacaca ttttaccgaa aaaccacaaa atttttttat gtctcccctt ttattgatat
    14821 agatgcggag tttgaatttg acctcacgtt gccggacgat cagctgatga tgcgtatcaa
    14881 tgatattcag aataatcgcc gtttttttgt cagcacctta tccggaaaac gacagatact
    14941 aaccgatgca agattggcac tgtatcttct tcggtttcca ctcgtaacct tgcaggtgat
    15001 attttttatt cactggcagg cattcagact gtggttaaaa aaattacatt atcataaaaa
    15061 aacggatttt cccgacttac aacgggaggt acatcatgca ggaagttatg tcaacgccat
    15121 tgccgcagag cgccgataac acaaaaaaaa ttatgcctgg tttttacgaa aaactggtac
    15181 tccgtacgtt cgaaggaatg catcaaggtt acatgcgcat ccatttgccg gacggacgaa
    15241 tgtatacgta cggtaatgcc aatgccgaat tgcaggcaac gatccaagtg cgcagtaata
    15301 atttttttaa gaaatgcgct ctctacggcg atatcggttt cggcgaagcc tatgtggatg
    15361 gtgattggga caccgacagc gttacccgtg tgattcgctg gtttttgctc aacgtcgaac
    15421 aagcgcctac catttcagga tccaagcgcc gcatcgtccc cgcaaatttt cttaaaggga
    15481 ttaaccgttt atatcatcgg ttacgagaaa ataccgtcag gcttgcgcgt atgaatattc
    15541 a
//
LOCUS       DAOQGF010000079        15536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1748_length_15536_cov_6.993347, whole genome shotgun sequence.
ACCESSION   DAOQGF010000079 DAOQGF010000000
VERSION     DAOQGF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1748_length_15536_cov_6.993347"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PK937_11020"
     CDS             <1..961
                     /locus_tag="PK937_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNH33146.1"
                     /translation="LTMGELAWGDVWYDGKTRNPWDTTRGSSGSSAGSASAVSAGLLP
                     FAIGTETWGSIVSPSTECGTSGLRPTYGRVSRYGAMALSWSMDKIGPIARSAEDCALI
                     FNAMYGPDGLDKTLVDAAFNYDASLKISNLRIGYLKSDMEKDSTRKAYYDTTLAVLRK
                     LGAKLTPIRLPQYPVYDMAIILSAEAGAAFDELTRSGKDDQLVRQIKNAWPNVFRASR
                     FIPAVEYIQANRIRTQLIQDMQNLMDTIDVYIAPPFEGDNLILTNLTGHPCVVVPNGF
                     TERNLPTSVCFMGKLFGEAEILALAKQYQDATDYHKRHPGLRF"
     gene            1155..3272
                     /gene="rnr"
                     /locus_tag="PK937_11025"
     CDS             1155..3272
                     /gene="rnr"
                     /locus_tag="PK937_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNH33147.1"
                     /translation="MLPEQKILQFLQSNAQSSFKSKDILRELHIRADQHDEAHGILQK
                     LVASGSIGKKGNKYFARAVSLTGTLSINKAGEGFVTVEGYEEDFFISPSRLKTAMNKD
                     TVMMIPYLRKRTGKRPEGEIIGIVERARTRIVGTVDSTGDFFVVIPDDRRLRRDIYVA
                     PEKTNGAKHGQKVVVSLEKWDDPSLNPEGSIIEIIGYPGDKGVDVLSVVKQHDIHFDF
                     PEAVEAECARIPATIPDDEIARRLDFRAALVFTIDPFDAKDFDDAISLDEGPQGHTIL
                     GVHIADVSYYVRENTALDREAYKRGTSTYLVDQVTPMLPEKLSNELCSLRPDEDKLTY
                     SCFMEIDAKGHVVDYDVVETVIHSKRRFTYEEVQQVIEGKPLDNLSPEVKKAVTDAYA
                     LSKRLTKKRMAEGSIDFDTPETKFKLDENGTPVECYRKERLDAHRLIEEFMLLANQYV
                     SKHIGLGSKKNELPFLYRVHDKPVKEKFENFVHLMKVLGHATHLPKSMDLLTPKHVQK
                     VIASVKGSKEDNLIEKVAIRSMAKAEYSDKNIGHFGLAFDYYSHFTSPIRRYPDLIVH
                     RMLKEYNAGMLRERIAWWSSKLPEMARHCSDRERVATEAERDSIKVKQAEFIERFLGE
                     EFDGIVSGVIQYGIFVEISQYLIEGLIHVRDLKGDYYVFDEKNYRLTGQRRKATFRLG
                     DRIRIKVARVNRDKREVDFTIVE"
     gene            3293..4390
                     /locus_tag="PK937_11030"
     CDS             3293..4390
                     /locus_tag="PK937_11030"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNH33148.1"
                     /translation="MRFPGINILDRYLIKKFLSVLFFALAGMVTIFVAVNYVENADRF
                     IDRKVPGNIIAEYYLNFIPHIITLTLPVDILLASLFSIGSLARFNELTAIKAGGRSLY
                     RVLFPIIVLALLVSAVNFYISEAIVPPANITKSEIWKSYVERASANRKVIGTDIHIYD
                     PSGTKVFIDKFDKRNRIISVMSIQQFYGTKLISRIDAGEATWDSTEAAWILRDVVERR
                     FTDSTETVIKHPTLAKRDLFFDPSDILRREQNPDEMNFIELRDFIHKLKRGGAQTERW
                     ETDYQLKFAYPLTCFFMVLFGAPLAASRKKSGAGINIFFTLVICLAYWSVIQTGRFMG
                     YNQSMDPVTAAWLGNSVFGVLSLLIFLRMKS"
     gene            complement(4398..4898)
                     /locus_tag="PK937_11035"
     CDS             complement(4398..4898)
                     /locus_tag="PK937_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH33149.1"
                     /translation="MRLVWMFLITAALPAYAQPDTTEQNLTRTRMAVVPFKNNTGSKQ
                     FEIITAGIAESITTALSGSGKFDFVERLQVDKALSEQDFQMTDLVDSKTVQSMGAILG
                     VEYIIIGDLQKIGTRFRINARRMRVSDGKLAESASASGEEDDLFDMQDEVAEKLIEQF
                     EQRNEK"
     gene            5244..6413
                     /locus_tag="PK937_11040"
     CDS             5244..6413
                     /locus_tag="PK937_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33150.1"
                     /translation="MRSALLKLLLMLSIGFTASAGTKTWNNASGGNWGTASNWLENAV
                     PGSGDTVYITLDGTYSVTYNLNFASVSAIYLGASSGIQTLINPGNANGVTGTMHINGN
                     GVFQMSSGTYSSSTVINYGTVRLIGGSFTFAPKIVNYDSVYISGTSIFVSGGLSNYDS
                     VFIAAGSFNLNGTDTSYAGKYLISFGATLNFNAGSHTLDANSSISGAGNVSVAATVNV
                     SGTYNITGTTTASNGTLLFNGTAGSLLNIGSTLAMSGGSVQVLTGDSLILGSATLNST
                     SSTLLVKAPIRFTTSFTMNQGVLRATDSTIKLLIGSGQTLTLNPTSTLRWQKITVENN
                     GTIDWTGGGTATLTVDSGLVINNYGLFDCKIASSPSLDHNQGALSRLNNYGIFRR"
     gene            6435..>15536
                     /locus_tag="PK937_11045"
     CDS             6435..>15536
                     /locus_tag="PK937_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNH33151.1"
                     /translation="MDADIYSYSGSTVDLQTGVTYFYRSLITTDADVTIASGATLYFT
                     GTSAAFDANSSISGAGNVTISSGTATFTGDVNIKGVSYFLSSSSMTFNASAGKLLTLG
                     DSVSIASGTINITSPQSITMRSLRQSGGTLNLTMNDTLVIDTLIQVNGTITGNSPVRI
                     KDWFNWLGGTQSATDSTVATIIQTTALFNSGTHTLSKRTLINRGTISWTLGHWDNNTG
                     AIIRNESGAIIDATAAGYYINYFGTAPAPKFINNGTFRVSSGSSYVYLNVDAINTNAS
                     YQIQSGTLQVSASATNVNTSWTLSNNRFAIFAGGTQTWDADSPISGTGTVRFTGGTHT
                     INSVYNISDSTDVATAVTFNGSAPSVGPYLGISGNATFNTKDSLILSRLTMLSSTATF
                     NTNDSVIVDRMVQSGGIMTGNSPIRVKTRYDWSGGYIYGSDSTVTFINSGIVNLTNNT
                     RQVQKRTFINEGTLNWNAAHWDWDYGAIFINHQGALINITGDWYWNNFPNSGSDPMIY
                     NYGTIHKSSGAGQSTINIPLYDSLATYEVASGTLAVQSNHTSKATNFNLSNGARYRIY
                     GSTQYIDTSNTINGSGILDFNGGTAYVASRIPSVDSVTVSGGTVYFNQPDTLQFSVLS
                     ITSGGILNGSATVKINTRFAFNSGTLSGTDSTNTLIIGSNAVLNASGLNFFSKRTIDN
                     YGTLNWTSSEWRVSNGAVFINRPGAIILTNSGSSYWHQTGSVPRVENYGTIRKSAGAN
                     TEWNVATNHYNGSRVDIKLNKLRFGGVNNATGAVIDIDSAATWQIDAANQTYDSSCTI
                     QGKGMILGDAFGGTIRLRGIINVTGPTMTLYGSTYYFDGPNGHIIKLPKRIIIGHTFA
                     ATNVYFDRSDTAKLEEVWMQNGNFGGTSIILIQDTLKFTNSAIVGTDSSQQIIIAPTA
                     AFLWLGTGSISKRTFNNYGTAIWTTPWGLDQNAVYNNMPGALTDWPLDGNTNWFTGTS
                     NNFNNYGTLRKSGGTGISSFEYYVNNYGTIEAWSGTLFFGTSGTHAGGSFILSPGSTI
                     DFAGGNHSFDASSNITGSGTVLISPTNFTMDGIYNIIGNTTIGGGTVNFDSTFSTSNR
                     LTITGGTVNFRMPSGHLLNAGNRLNVSGGTANFITTDSLKVDTLLVNGTAGGNNPWFV
                     RHYLQLTGTLSSNTPAQTLTLSPASVFDYNNGTLFRRVVNNYGTANWNNPVMFDAGAV
                     YNNMPGATTTLATDVNSNWMFAAAKPYLNNYGLLRKSGGTGISSFEYQISNYGTLQVD
                     SGLTILCYDSLVNFSTGIITGKGVLDVGTRFDNYGTIRPGASPGKLRFTGNINNKSTA
                     AYDAEISGKNIGQFDELRASGAATLAGTVNVNLLNGFLPALGDTFKIMNYASKSGGFG
                     AIQGLRTGGPYDFVPMYKDTALFLVTADSSNTPPVAVNDIVTASEDVPSTLSVLRNDT
                     DTNGDALRIVEILSGFPYHGTVVNGSGDTTIVYTSASEYSGSDSLQYVVADGRGGLDT
                     AAVKIAVNSVNDRPDAKDTTTTVTQYSVVRINVLATATDVENQTLSVSAVSRPKFGDA
                     QIDGTSSSVVYIPNMFYTGVDSFKFYVSDGSLADSATMFVTVTSGDSFLVTWSKFDAG
                     MENWSTVNDVSDSAYRSTGGNPGGYFTATDAVTGAWWYFNAPSKFLGDLSSVYDKTLR
                     YDIKQSPAGTDNSQPDVILIGNGVQLNYDFPANPRTTFTSFGLVFNETAGWKKTSGSV
                     PTQSELQNVLSNVTALRIRGEYASGTDSAGLDNVILEKAQNNNNPVVIMDTEEISEES
                     NITSAVLDNDTDPENHVLHIASISQPRHGTATINSGNLTITYKPDSNFAGVDTVMYYA
                     ADNYGGQSFGYLQITVNNINDAPTISSAVRDTAYNEDFGKVYVARLLDIFGDVDNGLT
                     FGGSTIASGATPYISNDSLYLLSVASFNGIATIELTANDGEYTIRDTFSVDITMQNDA
                     PYVASAIRDTTLAEDFGRIFYVNLSDHFADVESDSLSYTITAIATGVTAHIQHDSLYV
                     MSVNDFHTATATLSITAGDGQYSVSDTFDVNVTPVNDPLVQTTALNNQTLIENFDKTV
                     LGYLPNHFSDPDDATLSYTASVMSGNVVAYISGDTLYAESVAGFLGDAFLRITASDTQ
                     YTVADTLTVSVVPFNYTPTVVQPLRDTTWSEDIGKIFIAELNSHFTDVESSTLIYSGQ
                     VIAGSGTTTITNDSLYLQSAKDFYGNILVRITASDGALSVSDTLAVEITNVNDAPQIA
                     SHISDITKTVDFGKVYVEKGSNVFNDVDNDVLTMTATALQAGVNTEVSGDSLYIISIG
                     GFVGNVGILVTAHDAEYSVTDTFYVSVNPENFPPTLVQNLRDTTFAEDFAKTRVYRLN
                     SFFNDVDDINLSYSSQILSSGVSAIIAQDSLYLSGVADFNGIAVVRIQAQDSYGNSVA
                     DTINVIVTPVNDAPNRTHQITDMDLAQDFGKAYVDKLSAVFNDIDNGTLNYAVSTLNA
                     GVNGLIQNDTLYLISTNGFTGASTLRITASDGEYTVADTLIVNVFSTNHSPVLAQMLR
                     DTTLNEEFGRIRHSVLTQFFTDSDLDALTFTATAETGVSTSIQNDTLYLTSTSQFSGT
                     PKVIVSVSDGLVAISDTFTVTINSVNDAPSAFTLVTPVNNDLLTSLRPAFVWRKAIDQ
                     DGDVLSYSIEVSTNNTFASTVINTSTSDTSYAPASDLDSAQSYFWRITAHDGHGGNTV
                     SNVQSFTVDAKRPTLSLWFNQGPILKSYIDLQVYSSEVLSGAVQAQVTMKNTANTVTD
                     NRTVSLQRLGAGTLYTGKYQMNTEGSLTVAINAQDAAGNTRSVQRIYQVAAVSKSHTL
                     ALKSTSVLLSSNGRMKSSDGFILMGEDTEGELTEILVSTSVNIHGTIEISDALNLVIR
                     LDKEMLAEASVQSQFDKRQIGIYRKTDMGYEYVGGQSDGYEVRAKIAHYGTYVALYRA
                     GHVIVPDRLELAQNYPNPFNPSTTIEFGLTENGNVKIAVYNLLGQLVKTLVDEKREAG
                     YHKIVWDGRNAAGQLSASGVYIYRLETKSGFV"
BASE COUNT         4175 a         3344 c         3732 g         4285 t
ORIGIN      
        1 attaactatg ggcgaattgg catggggtga tgtgtggtac gacggtaaaa cgcgtaatcc
       61 ttgggatacg acacgcggat caagcggttc atcggccggc tccgcgtcgg cggtttcagc
      121 cggattgctg ccttttgcga tcgggactga aacctggggt tcgatcgtat ccccttcgac
      181 ggaatgcgga acgagcggtc tgcgaccgac atatggtcgt gtaagtcgtt atggcgctat
      241 ggcgcttagt tggtcaatgg ataaaatcgg gcctatcgcg cgttcagcgg aagattgtgc
      301 actgatattt aatgctatgt acggaccgga cggtttggat aaaactttgg tggatgcggc
      361 attcaattac gatgcttctt tgaaaatcag caatctgcgt atcgggtatc tgaaatcgga
      421 tatggaaaaa gattctacgc gcaaagcgta ttatgatacg acgctggctg tattgagaaa
      481 actgggtgca aaattgaccc cgatccggtt gccgcaatat ccggtctatg atatggcgat
      541 catcttatcg gcggaggcgg gggcggcttt tgacgaactc acgcgcagcg gtaaggatga
      601 tcaacttgtt cggcaaataa aaaacgcatg gcccaatgtg tttcgcgctt cgcgctttat
      661 cccggcggtt gaatatattc aagccaatcg cattcgtact cagcttattc aagatatgca
      721 aaacctgatg gacacgatag atgtgtatat cgctcctccg tttgaaggcg acaacctgat
      781 acttaccaat ttgaccggtc atccgtgtgt ggtcgtgcct aacggattta cagaacgtaa
      841 ccttccaacc agtgtgtgtt ttatgggaaa acttttcggt gaagctgaaa ttttagcatt
      901 agccaaacaa taccaagatg caacggatta tcataagcgg catccgggtt tacgtttcta
      961 accataactt actttcaaac caaaattttt agagccgccg ggtgtgatat taatacccgg
     1021 cggtttttta ttcagatttc aataaaaaca ttagtgtaaa cctttattcc gtcttatatt
     1081 ttcctgtttg atttgatata cttatcctgt atggaatatt tccatcgggt ggttgtttat
     1141 aaccggaaaa taacttgttg ccagaacaaa aaattttaca attccttcag agtaacgcgc
     1201 agtcttcttt caaatccaaa gatatacttc gtgaactgca tatacgggcg gatcagcacg
     1261 acgaggccca cggtatattg cagaaactcg ttgcttcggg gtctatcgga aaaaagggta
     1321 acaagtattt tgcccgtgcc gtgtcgttga cgggaacact ttcgatcaat aaagccggtg
     1381 agggttttgt gacggtcgaa ggatatgaag aagatttttt catctcaccg tctcggctca
     1441 aaaccgcgat gaataaagat acggtgatga tgattcccta tctccgtaaa cgaaccggta
     1501 agcggcccga aggcgaaatc atcggcatcg tagagcgtgc acggacgcgc atcgtgggca
     1561 cggtggacag taccggagat ttttttgtag tcatacctga cgatcgtcgg ttgcgtcggg
     1621 atatttatgt ggctcctgaa aaaaccaatg gcgccaaaca cggacaaaaa gtggtggttt
     1681 ccttagaaaa atgggacgat ccttcgctga atcccgaagg ctccattata gaaatcattg
     1741 gttatcccgg cgataaaggc gtcgatgtct tgtcggtggt caaacagcac gatattcatt
     1801 ttgattttcc ggaagcggtc gaagcggagt gtgcgcgtat cccggcaact ataccggatg
     1861 atgaaatcgc acggcggttg gattttcgtg cggcattggt ttttacgata gatccttttg
     1921 atgccaaaga ttttgatgat gcgatttcct tagatgaagg cccgcaaggg cacaccattc
     1981 tcggcgtaca tatcgcggat gtgagttatt atgtacgtga aaacaccgca cttgaccggg
     2041 aagcttacaa gcgtgggaca agtacgtatc tggttgatca ggtgacgccg atgttaccgg
     2101 aaaaactttc taacgagttg tgcagccttc ggcccgatga ggataaactc acgtacagtt
     2161 gttttatgga aattgatgcc aaagggcatg tcgtggatta tgatgtcgta gaaacggtta
     2221 ttcacagcaa acgacggttt acgtatgaag aagtgcaaca agtcatcgaa ggtaaaccgt
     2281 tggataatct ttcaccggaa gtgaaaaaag cggttaccga tgcgtatgct ttgtctaaac
     2341 ggcttactaa aaaacggatg gcggaaggca gtatagattt tgatacaccg gaaacgaaat
     2401 tcaagttgga tgaaaacggc actccggtgg aatgctaccg caaagagcgt ttggatgctc
     2461 accgtttgat cgaagagttt atgcttttag ccaatcaata tgtcagcaaa catatcggcc
     2521 tcggcagcaa aaaaaatgaa ttaccttttc tgtatcgcgt acacgataaa cccgtcaaag
     2581 aaaagtttga gaattttgta catctgatga aggtgctcgg tcatgcgaca cacctgccta
     2641 aatccatgga cttgcttaca cccaaacacg tgcagaaagt gatcgcatcg gtaaaaggtt
     2701 ccaaagagga caatctgatc gaaaaagtgg cgattcgctc catggccaaa gcggaatatt
     2761 ccgataaaaa tatcggccac ttcggtttag cgtttgatta ttattcgcat tttacttcac
     2821 ccatccgtcg ttatccggat ttgatcgtgc atcgcatgct caaagagtac aatgcgggta
     2881 tgttgcgtga acggattgcc tggtggtcat ccaaactgcc ggagatggcg cggcattgtt
     2941 ccgatcggga acgcgtcgct acggaagcgg aacgcgattc catcaaggtg aaacaagcgg
     3001 aatttattga acgctttttg ggcgaagaat ttgacggtat cgtcagcggt gtcatccaat
     3061 acgggatttt tgttgagata tcccaatacc tcattgaggg gcttatccat gtgcgcgatc
     3121 tcaaaggcga ttattacgtg tttgacgaaa aaaattaccg cctgaccggt cagcgacgaa
     3181 aagcaacgtt tcgtctcggc gaccggattc gcatcaaagt tgcacgcgtc aaccgcgata
     3241 aacgcgaagt tgattttacc atcgtggaat aaaaattgat ttcaattcac atatgagatt
     3301 tcccggcatc aatattcttg atcgttatct gatcaaaaaa tttctttcgg tattattttt
     3361 cgcacttgcc ggaatggtga cgatatttgt agcggtcaat tatgtcgaaa acgccgaccg
     3421 ttttattgac cgcaaagtgc ccggaaatat aatcgccgaa tattacctca actttattcc
     3481 gcacatcatc acgctcacct tgccggtgga tattttgctg gccagtcttt tttccatagg
     3541 ttcgctggcg cgtttcaacg aattgacggc gatcaaagcc ggcggccgaa gtttgtatcg
     3601 cgtactgttt ccgatcatcg tactcgcact gctggtcagt gcagttaatt tttatatcag
     3661 tgaggcgata gttcctccgg ccaatattac caaatcggaa atatggaaat cctacgtcga
     3721 acgtgcttca gccaatcgaa aagtgatcgg tacggatatc cacatctatg atccgagcgg
     3781 tacgaaggtg tttattgata aatttgacaa acgaaatcgc atcatttccg tcatgtccat
     3841 ccagcagttt tacggtacca aacttatatc gcgcattgat gccggtgaag cgacatggga
     3901 tagcacggaa gcggcgtgga tactgcgtga tgtcgtcgaa agacgtttta ccgatagtac
     3961 ggaaaccgtg atcaaacatc ccacgctggc caaacgcgat ctttttttcg atccgagtga
     4021 tatcctgcgc agagagcaaa atccggacga gatgaatttt atcgaactga gagattttat
     4081 ccataaactc aaacgcggtg gtgcacagac ggaacgctgg gagacagact atcagcttaa
     4141 gtttgcgtac cctcttacct gcttttttat ggtacttttt ggcgctccgc tggcagcctc
     4201 acgcaaaaaa agcggcgcag ggataaatat cttttttacg ttggttatct gtctggctta
     4261 ctggagtgta atacaaaccg gtcgtttcat gggatataat caatccatgg atccggtcac
     4321 ggcggcgtgg ctgggtaatt cggtgttcgg cgttctatcg ttgttgattt ttttgcgaat
     4381 gaaaagctga atagacgtta tttttcatta cgctgttcaa actgttcgat cagcttttct
     4441 gcgacttcat cctgcatatc aaacaaatca tcctcttcac cggaagcgct ggccgattcc
     4501 gccagtttgc cgtcgcttac acgcatgcgg cgcgcattga tacgaaatcg agtgccgatt
     4561 ttttgtaagt cgccgataat gatgtattcc acgccgagaa tagcccccat agactgcacg
     4621 gttttggaat ccacgagatc tgtcatctga aaatcctgtt ccgacagggc tttatctacc
     4681 tgcaggcgtt cgacaaaatc aaattttcct gagcccgata gtgcggtggt tatgctttca
     4741 gcaatacctg cggtaatgat ttcaaattgt tttgaaccgg tattgttttt aaatggtact
     4801 acggccatac gtgttctagt taggttttgt tctgtcgtat cgggttgggc gtatgccggt
     4861 aaagcagccg tgatcaaaaa catccatact aaacgcatgg gttattctcc tgaaatgtgg
     4921 tatgtgttta tttacgacag aaccatatcg taaacgctgc aaatattaag tttttttgtt
     4981 ttaaaaagca cgaaaatcgg actatacata gaaatacgta tttcggctga gcatgtcatt
     5041 ttctttgcct tggtataaac tataagttaa attttatcgg ttcttgcctc atcagttcta
     5101 ttccttagtt tctggcttgt atatcccatc tttatgggat ggatttcacg ttcagataaa
     5161 gcgactttgt acctatctca taaggttcgt ttaatatatt ttttattaca taatacatcg
     5221 tctttatcat aggaggaaga tatatgagat cagccttact gaagctgcta ctcatgcttt
     5281 ccattggatt taccgcatcg gcgggcacaa aaacttggaa taatgcatcc ggcggaaatt
     5341 ggggcaccgc atccaactgg ttggaaaacg ccgttcccgg ttccggtgat acggtttaca
     5401 tcacacttga tggaacttat tccgttacat acaatcttaa ttttgcatcg gtgtctgcga
     5461 tttatttagg tgcatcgtca ggtattcaaa cactgattaa tcccggaaac gctaacggag
     5521 tcaccggaac gatgcatatc aatggtaacg gcgtgtttca aatgtcgtcg ggtacttatt
     5581 cgtcatccac ggtgatcaat tacggtactg tacgattgat cggagggtcg tttacctttg
     5641 ctccaaagat tgtcaactat gacagtgttt atatttcagg tacaagtatc tttgtaagtg
     5701 gtggtttaag caattatgac agtgtgttta ttgcagcagg tagtttcaac ttgaatggaa
     5761 ccgacacgtc gtacgcagga aaatatctga tttccttcgg tgcgacatta aatttcaatg
     5821 caggctcgca tactctagat gctaattcat ccatcagcgg tgcgggaaat gtaagtgtag
     5881 cggctacggt gaatgtgagt ggaacatata atattacagg gacgacgacg gcatcaaacg
     5941 ggacattgtt gttcaatggt acggcggggt cactgttgaa tattggttcc acgcttgcaa
     6001 tgagcggcgg tagtgtgcag gtgcttacgg gtgattcgct gatattggga agtgcgacac
     6061 tgaatagcac aagctcgacc ttattggtaa aagcgccgat acgttttacg acatcgttta
     6121 cgatgaatca gggtgtattg cgtgctacgg attcgacgat caagttgttg attggcagcg
     6181 gtcagacgct tacgctcaat cctacatcaa cgcttcgttg gcaaaagatc acggtggaga
     6241 ataatggcac gattgattgg accggaggcg gtacggccac gctgacggtg gactccggtt
     6301 tggtgatcaa taattatggg ttatttgatt gtaagatagc cagttcaccg tcattggatc
     6361 ataaccaagg tgcactttca cgtttgaata actacggaat attccgccgt taggttaatg
     6421 caaccaactt tactgtggac gcagatattt attcatacag cggttctacg gttgaccttc
     6481 aaactggggt aacgtatttc tatcgttcgc tgataacaac ggatgcggat gtgacgattg
     6541 cctccggagc tacattgtat tttaccggta cttcagccgc gtttgatgct aattcatcca
     6601 tcagcggtgc gggaaatgta accataagca gcggaacggc tacgtttaca ggagacgtaa
     6661 acattaaagg tgtttcgtat tttttgtctt cgtccagtat gacatttaat gcttctgcag
     6721 gaaaattact gacgctcggc gatagcgttt ccatagccag tggcacgata aacatcacgt
     6781 caccgcaaag catcactatg cgaagcttgc gtcaatccgg cggtacgctc aatttgacta
     6841 tgaacgatac attggtgatt gacacattga ttcaagttaa cggtacgatc acaggcaatt
     6901 ctccggtacg cataaaagat tggtttaatt ggttaggagg cacacaatcc gcaacggatt
     6961 caactgtggc aaccataatc cagacgaccg ctctctttaa ctcaggtact catacactgt
     7021 ctaaacgaac actgatcaac cggggaacga tttcatggac attagggcat tgggataata
     7081 acaccggcgc aattattcgt aatgaatcag gcgcaatcat agatgcgacc gctgcaggat
     7141 actatatcaa ttattttggt acagcaccgg cgccgaaatt tattaataac gggacatttc
     7201 gtgtgtcctc cggtagtagt tatgtctacc tcaatgttga tgccataaat acaaacgcgt
     7261 cataccagat tcaatcggga acattgcagg tatccgccag tgctaccaat gtcaatacgt
     7321 cgtggacttt gagtaataat cgtttcgcca tctttgctgg cggtacgcaa acatgggatg
     7381 cggattcacc tatcagtggt acaggaacgg ttcgtttcac aggcggaacg cacacgatta
     7441 acagcgtata caatatttct gacagtacgg acgtcgctac tgcggtgaca tttaatggtt
     7501 ctgcgccgag cgtagggcca tatcttggca tttcaggtaa cgcaacgttt aatacaaaag
     7561 actcgctgat attgtcgcgg ttgacgatgt taagcagtac ggcaacgttt aataccaacg
     7621 attcggtcat agtggatcgt atggttcagt cgggtgggat tatgacaggt aactcgccga
     7681 tacgtgtcaa aacgcgatac gactggagcg gtggatatat ctatggaagt gattcgacag
     7741 taacgtttat caatagcggc atcgttaact taaccaacaa tacacgtcag gttcagaaaa
     7801 gaacgtttat caatgaagga acgttaaatt ggaatgcagc gcattgggat tgggattacg
     7861 gtgcgatctt catcaatcat cagggtgcat tgatcaatat caccggagat tggtattgga
     7921 ataatttccc taatagcggc tctgatccga tgatctataa ttacgggact attcacaaat
     7981 cgtcgggcgc gggtcaatct acaatcaata taccactgta tgattctttg gcaacctacg
     8041 aagtggcgtc tggaacgtta gccgttcagt ccaaccatac atctaaagca acaaatttta
     8101 atttatccaa tggtgcacgg tataggatat acggaagcac tcagtacata gacacttcaa
     8161 acacgatcaa tggttccggt attttggatt ttaatggcgg tacggcctat gtcgcttcac
     8221 gaattccttc tgtggatagt gttactgttt caggtggaac agtgtatttt aatcagccgg
     8281 acacactcca attcagtgtg ctttcgatca cgagtggcgg catcctcaat ggttcggcta
     8341 cagtaaaaat aaatactaga tttgctttca actctggtac tctctccggt acggattcca
     8401 caaatacatt gattatcgga tcaaacgctg ttttgaatgc atctggtttg aattttttca
     8461 gcaaacgtac tattgataat tacggaacgt taaactggac ttcgagcgaa tggcgtgtta
     8521 gtaacggggc ggtattcatc aatcgtccgg gcgctattat tttaaccaac tcaggatcta
     8581 gctattggca tcaaacgggg agtgtgccgc gcgttgaaaa ctacggaact atccgtaaat
     8641 cggcgggtgc aaatacggaa tggaatgttg cgaccaacca ctacaatggt tcacgtgttg
     8701 atatcaaact caacaaactt cgttttggcg gagttaataa cgctacaggt gccgtgattg
     8761 atattgattc cgcggctact tggcaaattg atgcggctaa tcaaacgtat gatagttcgt
     8821 gcacgataca aggtaaaggt atgattttag gtgatgcgtt tggcggaacg atacgactaa
     8881 gaggcatcat caatgttacg ggtcctacga tgacgcttta cggcagcacg tattattttg
     8941 acggaccgaa tggtcatatc ataaaattac cgaaacgaat tattattgga catacgtttg
     9001 cggctacgaa tgtttatttt gacagatccg atacggccaa actggaagaa gtttggatgc
     9061 agaacggcaa tttcggaggc acctcaataa ttctaattca ggacactctt aaatttacca
     9121 acagcgctat cgtgggaaca gattcaagcc agcaaataat tatcgctcct acggcggcat
     9181 tcctatggct tgggactggc agtatttcta aaaggacatt caataattac ggaacggcga
     9241 tttggacaac accttgggga cttgaccaga acgcagttta taataatatg ccgggggctt
     9301 tgaccgattg gccgctggat ggaaacacca actggtttac cggaacttcg aataatttta
     9361 ataattatgg gacacttaga aaatccggtg gtaccggtat atcaagtttc gaatattacg
     9421 tgaataacta tggtacaatc gaagcttggt cgggtacgtt gttttttgga accagcggta
     9481 cccatgccgg aggtagtttt atactttcgc caggctcgac gattgacttt gccggaggaa
     9541 accactcttt tgacgcgtcg agcaatatca caggcagtgg taccgtactc atcagtccta
     9601 ctaattttac gatggacggt atatataata ttatcggtaa tactacgatc ggtggaggta
     9661 ctgtcaattt tgacagcaca ttcagtacat ccaacaggct aaccatcacc ggcggtacgg
     9721 ttaatttcag aatgccatcc ggtcatctcc tgaatgctgg aaatagactg aatgtttccg
     9781 gcggtacggc aaattttatt acaaccgact ccctgaaagt agatacactg ctggttaatg
     9841 gaaccgccgg tggaaataac ccgtggtttg ttcgtcatta tttgcagttg acaggtacat
     9901 tgagttccaa tactcctgca caaacactga cattgtcgcc ggcatcagtt tttgattata
     9961 ataatggaac gctgttccga cgcgtagtca acaactacgg tactgccaac tggaataatc
    10021 ctgtaatgtt tgacgcagga gctgtgtata acaacatgcc cggtgcgacg acgacactgg
    10081 caaccgatgt gaattctaat tggatgtttg cagctgccaa accgtatcta aacaattacg
    10141 gattgctgcg caaatccggc ggcacgggca tttcgagttt tgaatatcag atcagtaatt
    10201 acggtacatt acaggttgat tccgggttaa cgatcttgtg ttatgacagt cttgttaact
    10261 tcagtaccgg tattatcacc ggcaaaggtg ttcttgatgt cggtacgcgt tttgataatt
    10321 acggtacaat acgtcccggc gcttcgcccg gcaaactgcg tttcaccgga aacatcaata
    10381 ataagagtac ggctgcttat gatgccgaga ttagcggaaa aaatataggc caatttgatg
    10441 aactgcgcgc aagcggcgcc gcgacactcg caggaacagt aaatgtaaat ttacttaatg
    10501 ggtttttgcc ggcgcttggc gacacgttta aaatcatgaa ttatgcgagt aagtccggag
    10561 gtttcggagc gatacaagga ttgcgtaccg gcgggccata tgattttgta ccgatgtata
    10621 aagacacggc gctttttctt gtgactgcag attcgtccaa tacacctccg gtagcggtca
    10681 atgacatcgt gacggcttcg gaagacgtcc catcaacact ttctgtttta cgtaacgata
    10741 cggataccaa tggtgacgcg cttcgtattg tcgaaattct ctccggattc ccgtatcacg
    10801 gtaccgtggt aaatggtagt ggcgacacga cgattgttta tacgtccgct tcggagtatt
    10861 ccggttcgga tagtctgcag tatgtggttg cagatggtcg cggcggtttg gatacggcgg
    10921 cggtaaaaat cgcggttaac agcgttaatg atcgtcctga cgcaaaagat acaacaacga
    10981 cggtcacaca atatagcgtc gtacgcatca atgtactggc aacagcgacg gacgtcgaaa
    11041 atcaaacact ctcggtgtca gcggtatcgc ggcctaagtt tggcgacgca cagattgatg
    11101 gtacatcctc ctctgttgtg tacataccaa acatgtttta taccggcgtg gatagtttca
    11161 aattttatgt ctccgacgga tcattggcgg attcggccac gatgtttgtt acagttacat
    11221 ccggcgattc attccttgtc acgtggagta agtttgacgc gggtatggaa aattggtcaa
    11281 cagtcaatga tgtatcggat tccgcatacc gttcgaccgg aggaaatccc ggcggctatt
    11341 ttacagcaac cgatgccgta acaggcgcct ggtggtattt taacgcacct tccaaatttc
    11401 tcggcgacct cagctccgtt tatgataaaa cactgcgtta tgatatcaaa caaagccctg
    11461 caggaacgga caattcgcag cccgatgtga ttctgatcgg taacggtgtt cagctaaatt
    11521 atgattttcc ggcaaatccg cgtactacgt tcacgagttt tggtttggtg ttcaacgaaa
    11581 cggcgggatg gaaaaaaaca tcagggtctg ttccgacaca atctgaattg caaaacgtac
    11641 tgtccaatgt tacagctctg cgtattcgtg gtgaatatgc ttcagggacg gatagcgccg
    11701 gtttggataa tgttattctg gaaaaagcgc agaataacaa taatcctgtt gtcatcatgg
    11761 ataccgaaga aatttcagaa gaaagcaaca taacatcggc tgttcttgat aatgatactg
    11821 acccggaaaa tcatgtgctc catattgctt cgatttccca accgcggcat ggcacggcta
    11881 cgattaactc gggtaatctg acgatcacat acaaaccgga ttccaatttt gccggtgtgg
    11941 atactgtgat gtattatgcg gcggataatt acggcggaca gtccttcggc tatctgcaaa
    12001 tcacggttaa taatatcaac gatgcaccga ccatttcgtc agcggtacgt gacacggcgt
    12061 acaatgaaga cttcggcaaa gtttatgtgg ctcgcttatt ggatattttc ggtgacgtgg
    12121 acaatggact cacgttcggt ggaagtacga tcgcatccgg cgcgacacct tacatttcca
    12181 atgacagcct ctacctctta agcgttgctt catttaatgg tatcgcaacg atcgaactta
    12241 cggcgaacga cggtgaatat acgatccgtg atacttttag tgtggatatc acgatgcaaa
    12301 atgatgcgcc gtatgtcgca tccgcgatca gagatacgac cctagcagag gattttgggc
    12361 gaatttttta tgtcaatcta tccgaccatt ttgcggatgt agagtctgac tcgttgagct
    12421 atacgataac ggccatcgcc acaggtgtaa ccgctcacat acagcatgat tcgttatatg
    12481 tgatgagtgt taatgatttt catacggcca cggctacact aagtatcaca gcgggcgacg
    12541 gacagtactc tgtatccgat acgtttgatg taaatgtaac gccggtcaac gatccgctcg
    12601 tgcaaacgac agcgctcaat aaccaaacgt tgatcgaaaa ttttgacaaa acggttttag
    12661 gttatttacc aaaccatttt tctgatccgg atgatgcgac gctttcatat accgcgtccg
    12721 taatgagcgg taatgttgtt gcctatattt ccggtgatac gttgtatgcc gaaagcgtag
    12781 ccggattcct cggtgatgcg tttttgcgga tcacggcttc cgatactcaa tatactgttg
    12841 cagatacgtt gacggtttct gttgtcccgt tcaactatac acccacggtg gtgcaaccgt
    12901 tgcgcgatac gacgtggagc gaggatatcg gaaaaatatt tatcgcagag ctcaattcgc
    12961 acttcacaga tgtcgaatct tcgacgctta tttatagcgg tcaagttatc gccggtagcg
    13021 ggacaacaac aatcactaat gatagtttgt atttgcaaag tgcaaaagat ttttacggta
    13081 atattcttgt tcgcataaca gcctcggacg gtgcactatc cgtcagcgat acgttggctg
    13141 tagagataac caatgtgaat gatgcgccgc aaatcgcatc gcatatatca gacatcacta
    13201 aaacggtgga ttttggaaaa gtatatgtcg aaaaaggttc gaatgtattt aacgatgtgg
    13261 acaatgatgt gcttacgatg acagcgaccg ctttgcaagc gggtgtcaat acggaagtct
    13321 ccggtgacag tctttatatc atcagtattg gtggttttgt gggtaatgtg ggaattcttg
    13381 taacagcaca tgacgcggaa tattcggtga cagatacatt ttatgtcagc gtcaatccgg
    13441 aaaacttccc gccgacgctt gttcaaaatt tgcgcgatac cacattcgcc gaggatttcg
    13501 ccaaaacgcg ggtgtaccga ttaaacagct ttttcaatga tgtggatgat attaatctga
    13561 gttattcatc gcaaattctt tcttccggcg tttccgcgat catcgcacaa gattcgctat
    13621 atcttagcgg agtggccgat tttaacggaa tagccgtcgt gcgtatccag gcgcaagata
    13681 gttatggaaa cagcgtcgcc gacacgatca atgtgatcgt tacaccggtc aatgatgcgc
    13741 cgaaccgtac ccatcagatc acagacatgg atttagcaca ggatttcggt aaagcttacg
    13801 ttgataaatt atcggcggtt ttcaatgaca tagataatgg aacactcaat tatgccgtat
    13861 cgacgctcaa tgccggggtc aacggcctga ttcaaaacga tacgctgtat cttatcagta
    13921 caaatggttt tacaggcgcg tccacgttac gtatcacggc atcggacggc gaatatacgg
    13981 ttgccgatac gctgatcgtc aatgtgtttt ctaccaatca ttcgcctgta cttgctcaga
    14041 tgctgcgcga tactacgctc aatgaagaat ttgggcgcat acgtcacagt gttttgacgc
    14101 agttttttac ggattcggat cttgatgcgt tgacctttac ggcgacggcg gaaacaggcg
    14161 tttcaacttc catacaaaat gatacactgt accttacaag tacttcccaa tttagcggta
    14221 cgccaaaggt tattgtatcg gtttcggatg gcttggtagc gatttcggat acgtttaccg
    14281 tcacaattaa ttctgttaac gatgcgccgt cggcgtttac tttagtcacg cccgtaaata
    14341 atgacctgct tacatcgctt cgtcctgctt ttgtttggcg caaagccata gatcaggatg
    14401 gtgatgtgct gtcatactcg atagaggtat ccacgaataa tacgtttgct tctacagtga
    14461 tcaatacttc gacgtccgat acgagctacg caccggcatc cgatctggac agcgcacagt
    14521 cgtatttctg gcgcatcacg gcgcatgatg gccatggcgg gaacacggta tccaacgttc
    14581 aaagttttac ggtagatgcc aagcgcccaa cgctcagtct ttggttcaat caaggcccga
    14641 ttttgaaatc atacattgat ttacaggttt attccagcga agtactctca ggagctgtgc
    14701 aagcgcaagt aacgatgaaa aacacagcaa atacggttac ggataatcgt accgtatcac
    14761 tccagcgttt gggcgcgggt acgctttata ccggtaaata tcaaatgaat acggaaggca
    14821 gccttactgt agctattaac gcacaagacg cggccggcaa tacgcgttcg gttcagcgaa
    14881 tttatcaggt tgctgcagtt tcaaaatcac acacactggc actgaaatca acttcggttt
    14941 tactttcgtc taatgggcgg atgaagtcgt ccgatggatt tatcctcatg ggtgaagata
    15001 cggaagggga attaaccgaa atattggtaa gtacgtctgt aaatatccac ggtacgatag
    15061 aaataagtga tgcattaaac ttagtgatcc gacttgataa agagatgctc gcagaggcat
    15121 ccgtgcaatc gcaatttgac aaacgacaaa tcggtatcta tcgaaaaacc gatatgggat
    15181 atgaatacgt tggcggtcaa tcggacggat acgaggtgcg tgcaaagatc gctcactatg
    15241 gtacatacgt cgcgctgtat cgcgcagggc atgtcattgt gccggatcgt ctggaactcg
    15301 cgcaaaacta tcccaatccg tttaatccgt ccacgacgat tgagtttggc cttacggaaa
    15361 acggaaacgt taaaatcgca gtgtacaatc ttttaggtca actcgtcaaa acactggtgg
    15421 acgaaaaacg cgaagccggt tatcataaga tcgtatggga tggccgtaat gcggcaggtc
    15481 agctttcagc aagcggtgtg tatatttacc gtcttgaaac aaaaagcgga tttgtc
//
LOCUS       DAOQGF010000080        15400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1768_length_15400_cov_6.573412, whole genome shotgun sequence.
ACCESSION   DAOQGF010000080 DAOQGF010000000
VERSION     DAOQGF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1768_length_15400_cov_6.573412"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PK937_11050"
     CDS             <1..1104
                     /locus_tag="PK937_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002738755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNH33152.1"
                     /translation="PLPVRLFGVRLEKIEEAQTQSNSEIVNKEVQKIRRQAESLPKNS
                     VVIMEAKHELQRLEDENFWSNLTSDKIEFLEAVVKPLLRTVSDVDFKAMRFEKDIVEV
                     SLAHLAGETEKFEALKESIIEEIGELPLSINIVAREQELIRQAQSNHYWATINEEKFE
                     ELIQKLSPLMKFREAVIPLAPAKFNLKDIVSEKEFVEFGPQHEALSVAKYRELVEKKI
                     NELVSSSPILQKLKQGQEITTDEAEQLAEELHNEHPHITIDLLRKVYNHRKAALVQFI
                     KHILGIEQLETFAETVTKAFDDFVSKHSYLTSRQIQFLDLLKNFVLEKGDVSKRNLIE
                     SPFTMLHPEGIRGIFNQKEIDEILSLTEKVLAA"
     gene            1105..>2063
                     /locus_tag="PK937_11055"
     CDS             1105..>2063
                     /locus_tag="PK937_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH33153.1"
                     /translation="MLQNNAQLKSLIDKLWQNFWEGGIANPLTAIEQITYLIFMKRLD
                     DLEAKRERDAEFTGEKYVSRFAGKFNIPGSNESIDKNELRWSVFKHKPADEMLMHVQM
                     KVFPFLKELNGETSPFTKHMANAVFIMPKANLLVSAINIVEDIFKEIEKDATEGGHAF
                     QDIQGDVYEMLLSEIATAGKNGQFRTPRHIIKLMAELVAPQLGQRIADPACGTGGFLL
                     GAYQYILTDLVRKKDPAKLGKDEDGFERAAISAVLTQKVKTILDQSFVGYDIDTTMVR
                     LGLMNMMMHGIDEPKIDYKDTLSKGYNEDSRFDIVLANPPFTG"
     assembly_gap    2064..2163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2164..2532
                     /locus_tag="PK937_11060"
     CDS             <2164..2532
                     /locus_tag="PK937_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH33154.1"
                     /translation="VSTAILIFTKGGETNHVWFYDMQADGYTLDDKRNKIAESDLPDI
                     VQRYKTRDAKKDSDRKLKYFMVPKKEIVENNYDLNLSTYKEEVYEEVSYEKPNVIFSK
                     LESMEADIQKGLAELKEIVW"
     gene            2606..3064
                     /gene="tnpA"
                     /locus_tag="PK937_11065"
     CDS             2606..3064
                     /gene="tnpA"
                     /locus_tag="PK937_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNH33155.1"
                     /translation="MAGTYSQIYLQAVFAVKCRENLIAKSWKDELHKYISGIIKEKEQ
                     KPIIVNGMPDHIHAFIGLKPSMRISDLVRDIKNNSSKFINEKGFVRGKFSWQEGYGVF
                     SYSHSHIERVYNYILNQEEHHRKKTFKDEYLGLLRKFEIDFKEEYVFEFI"
     gene            3051..3200
                     /locus_tag="PK937_11070"
     CDS             3051..3200
                     /locus_tag="PK937_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33156.1"
                     /translation="MSLSKYITPSGLGSWVSLVCFNHANPSGLLSISMEADIQKELAE
                     LKEFV"
     gene            3200..4327
                     /locus_tag="PK937_11075"
     CDS             3200..4327
                     /locus_tag="PK937_11075"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNH33157.1"
                     /translation="MMFEKLGDIATIKTGKYDVNHSTPDGEYVFYTCAFGQFKSPTYS
                     FEGPAIILPGNGANVGEVFYYDGKFEAYQRTYVVQNFKADPNYIYAYFKAQWKNSLRN
                     KQFGSATNYIRLNNLTDFKIPLPPLPDQLHIANLLSKAENLIAQRKESIRLLDEFLKS
                     TFLEMFGDPSSNRKQFPKGSIRDVVAEVKYGTSSPAEDEGLYPYLRMNNITPDGYMDY
                     SKLKYINIVDEKEKEKYVVRKGDLLFNRTNSKELVGKTGVFNEDSEMIIAGYLIRVRT
                     NEKANPWFLWGYLNSIHGKQTLLGMCKSIVGMANINAQELQGIKILIPQIELQTQFAQ
                     IVKKTEALKTQYQQSLQELENLYGSLSRKAFRGELGVKEMA"
     gene            4538..4936
                     /locus_tag="PK937_11080"
     CDS             4538..4936
                     /locus_tag="PK937_11080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33158.1"
                     /translation="MPIEYSFDKRPSADQIIALYDRSGLPRPTQDKERIQKMYDHSDL
                     IVTAWDGDLLVGVSRSITDWVWSCYLADLAIHPDYQKNGIGKELVRLTKEKVGEEVMI
                     LLLSVPTAMTYYPKIGMTKEDRAFTILRTK"
     gene            5029..5532
                     /locus_tag="PK937_11085"
     CDS             5029..5532
                     /locus_tag="PK937_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33159.1"
                     /translation="MKNPFEIEKTYITEYSPSRITAIVQETLEKKNRMVFFSLKTFTG
                     AIKENHFRFKENVWATNNGMIYPTIQGTILGDNPTKIHIHITPRYGWIFSFALCALVF
                     LCAAFFTDRMSVDGIESAPTLYERSAFAIFGAGIPMLLCYVTCLRPIQHAERWIIKKL
                     DLKEITS"
     gene            5655..6098
                     /locus_tag="PK937_11090"
     CDS             5655..6098
                     /locus_tag="PK937_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33160.1"
                     /translation="MIELPILKLMPAIAKYFIRCALVFLLLSILIQTLTQVGPHWGWT
                     TIHNLQPVFFHFFLFGWVMQMIIGVSLWMFPWQDKAQFILRDRVGWFALLTINSGLVL
                     RGVCEPFLDPSSGDALRFALTASVILMWLGCVVYAAHIWQRVRSK"
     gene            6100..6525
                     /locus_tag="PK937_11095"
     CDS             6100..6525
                     /locus_tag="PK937_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33161.1"
                     /translation="MDRLSIWWIRGALIYFVVGVTLGALLLLHKTWSWDMLWWRLLPL
                     HIYTMLLGFMIPFIIGVAHWILPRNDGPHDRGNPKLVITAWILWHTGLIAGLTSEITG
                     SALSAITLSVALCNIAAVMIMFTQLWQRLRLFKQIYNGK"
     gene            6601..7614
                     /gene="hemH"
                     /locus_tag="PK937_11100"
     CDS             6601..7614
                     /gene="hemH"
                     /locus_tag="PK937_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNH33162.1"
                     /translation="MRSEVAQKTAIVLLQLGGPDNLDAVEPFLYNLFCDPDIIDLPGA
                     FLFRKGLARLISSRRSPAVRELYKNIGGRSPILPQTEEQAQALQLRLDTSGHQCTVFI
                     AMRYWHPMTDAIARSIAQGGYDRILLLPLYPHYCKATTGSGINEWRRCAKKYQIDHIP
                     TQTVASYFDHPDYIHAIVDNIKKALTQLPEDARAKTHLVFSAHGTPMKLVRDGDPYSI
                     HIRRTYEAVLSAGNFGLPHHLCFQSKVGPQQWLEPSLVQTIADLSAQKVSHMLVVPVA
                     FVTEHIETLSEINIEVRHEAEQAGVRYFDMMPALRDHPSFTACLYDLTLKALHGAWPV
                     SQS"
     gene            7744..8652
                     /locus_tag="PK937_11105"
     CDS             7744..8652
                     /locus_tag="PK937_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH33163.1"
                     /translation="MRYVDRFPDPHSRRFDKAEWRDEHGTANVIIHCQSSGIAFPKHW
                     GPLSLKTTLSGHEYYVTRHSTHRVTPERLLILNEDTTYASYIRDGETAESFTINFSAD
                     FIRTVLRDSLSDGMQKLDNPFSDDAPSVQFPEYTLPYHHAIRVRLNRIRRCLTMPADL
                     NGRVDEELSFLYEAMCTLSVSENKKIRDLPFQKASTREEMYRRLQRVRDYMDACYAEP
                     INLHDLSRVACLSVHHLIRLFKSHFLCTPHQYLIRCRLLEAKRLTETTSLRIEDIARR
                     VGFEDRSSFSRLFRHHFDKNPSDRTQ"
     gene            8737..9231
                     /locus_tag="PK937_11110"
     CDS             8737..9231
                     /locus_tag="PK937_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33164.1"
                     /translation="MLMRSFIVSLFFIWTKSFAQMPMDSSAFDFWVGEWTATWSNADG
                     STGKGKNRIVKILDGKVIQEHFEDESGFKGTSISVYTPAQKKWRQAWADNQGGYFDFE
                     GDISDGKKIFKTKMHEANGKQYMSRMLFYDIKTDAFTWDWEKTEDGGKTWALQWRIQY
                     ARKK"
     gene            complement(9251..10306)
                     /gene="arsB"
                     /locus_tag="PK937_11115"
     CDS             complement(9251..10306)
                     /gene="arsB"
                     /locus_tag="PK937_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNH33165.1"
                     /translation="MEKVSKLSFIDRYLTLWIFIAMLIGVFSGYFFSSIVGFWNRFQS
                     GMTNIPIAIGLILMMYPPLAKVKYEELADVFRNKKVLLLSLVQNWLIGPVFMFLLAVV
                     FLRDYPEYMAGLVMIGLARCIAMVIVWNELAHGDTEYAAGLVAFNSIFQVLFFPVYAY
                     LFLTLFPSLLGLQAFAVDITIMQIAESVFIYLGIPFLAGMLTRMILLKAKGRVWYHEK
                     FIPKISPLTFIALLFTIVVMFSLKGEYIVTIPFDVLRITVPLVIYFVVMFLISFYLSR
                     RSGADYAKSATLSFTAASNNFELAIAVAIAVFGISSGAAFAAVIGPLVEVPVLVSLVR
                     VALWIKSKYYDVPTGAN"
     gene            complement(10331..10753)
                     /locus_tag="PK937_11120"
     CDS             complement(10331..10753)
                     /locus_tag="PK937_11120"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNH33166.1"
                     /translation="MKKRVLILCTGNSCRSQMAEGFLKSFDASLEVYSAGTRPSSQVH
                     PKAVKVMGEIGIDLSGNYPKSTDLFLDKPFDYVITVCVNAQEQCPVFTGTVSHRLHIG
                     FDDPAEASGAEEHVMKEFRRIRDEIKKDFFEFYQKSLR"
     gene            complement(10795..11139)
                     /locus_tag="PK937_11125"
     CDS             complement(10795..11139)
                     /locus_tag="PK937_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH33167.1"
                     /translation="MTIQNYAEVFKALSDPNRLRILKMLEQRTLCVCEIRDVLKLAPS
                     TVSQHLKILKKNAYITEEKKGRWVYYSLNTKPLYPEITALRILLHTALNDTAQVQSDV
                     KKIQSSKVITCS"
     gene            11861..12145
                     /locus_tag="PK937_11130"
     CDS             11861..12145
                     /locus_tag="PK937_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33168.1"
                     /translation="MSISQPESRPLINITELFNSDPEWAAKRDKAIKKLYDGNTQEFN
                     AFMSKLEPMRDWKDVMDAVEAEFMRKKIKQDSKEATGLTDVLFKRYFPSY"
     gene            12323..12565
                     /locus_tag="PK937_11135"
     CDS             12323..12565
                     /locus_tag="PK937_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNH33169.1"
                     /translation="MAYIIAEPCIGVKDTACVKVCPVDCIYDGDGWDQLYIHPDECID
                     CGACEPECPVEAIFAAENTPDKWKSFIQKNADQFKQ"
     gene            12681..14627
                     /locus_tag="PK937_11140"
     CDS             12681..14627
                     /locus_tag="PK937_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH33170.1"
                     /translation="MKKITLPAFIFPCALILLDYVTNKSTVTFNTNRILAYTNGFIGS
                     IAFWTFYQLASAYTLHHYREHKRVRMLFIVGQILFALVFTATISVSLGYYYEFGNLPN
                     KFAVAYLMDEWTDAVSLITGYISMPLMFLLISVFAAIYACHYFVIRHIAQRMHTSTPA
                     SVKKVLAILAIITAASLLGGFFSKKLSATLPMDFGSLQAVAKYAFGDKSGKGYSVPQR
                     IILPENRTARKPSNVLLIINETLRADFIGYQTLANPIPPASRMTDAIASDSLSFVFKR
                     ARSNSSSTRVSLPAILMGMIPTTATSADYHRMPTLWNVSKALGYRTFFVTPQNWDWQN
                     LDVFFLNKQVDQSITAKDIGPPYVNNAGVEDHRILDYTHSIFQEFKKNKNPFFGVIQF
                     NCTHQPYNPGDPHTALSPNSTERYAAAVRYLDSLIAQIYIQLNEAGLKDETLIIVTSD
                     HGENFKHRGLARLNNLYDEVIRIPLWVSYPRKYYQTPEGKARFHAWKQNEYSAVQNAD
                     IFPTLLSYWGVDKTSLQGYSYQGMRWEDPADSLRILSGTNTGDIRKWNREEFFLLRHH
                     VKFLATDKAGPQIYNLQSDPYEEHNLWDDLSFREAHRPWIHQYISDHAVLAELAKRMK
                     INLGNGAAIHPSDTSFSRADIDLK"
     gene            14691..15143
                     /gene="rpiB"
                     /locus_tag="PK937_11145"
     CDS             14691..15143
                     /gene="rpiB"
                     /locus_tag="PK937_11145"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNH33171.1"
                     /translation="MKIAIGCDHGGFPLKATVIETVQKAGHETMDLGTFSSDPADYPD
                     YSEKVAQAVQTGLADRGIVICGSGVGATVAANKFKGIRAGLCHDTFSARQGVEDDDMN
                     VLTLGARIIGPLLAIEVIQAFLKARFSNAERHLRRLNKVKGFEENFGK"
     gene            15177..>15400
                     /locus_tag="PK937_11150"
     CDS             15177..>15400
                     /locus_tag="PK937_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNH33172.1"
                     /translation="MEKPYPPVYYADYLELNQLLGAQHPKSDAYGKPAHDEMLFIIIH
                     QAYELWFKQILHEVDSVARLFAEDHIDEKN"
BASE COUNT         4524 a         3428 c         3276 g         4072 t
ORIGIN      
        1 cctttgcctg ttcgcttgtt tggtgtgcga ttggaaaaga ttgaagaagc ccaaactcaa
       61 agcaattccg aaattgtaaa taaagaagtt cagaaaatca gaaggcaggc cgaatcactg
      121 cctaaaaact cggtagtgat tatggaagcc aaacacgaac tgcaacgctt agaagatgaa
      181 aacttctgga gcaacctgac ttctgataaa atagaatttt tagaagccgt tgtaaaacca
      241 ttgcttcgca cggtttcaga tgttgatttc aaagccatgc gttttgaaaa ggacattgtg
      301 gaagtttcat tggctcattt ggctggtgaa actgaaaaat ttgaagcgct gaaagaaagc
      361 atcattgaag aaattggcga actgcctttg agcatcaaca ttgttgcaag agagcaggaa
      421 ttgattcgtc aagctcaaag caatcattat tgggcaacta tcaatgaaga aaagtttgaa
      481 gaactgattc agaaactttc gcctttgatg aaattccgtg aagcggtaat tccattagct
      541 cctgcaaagt tcaacctgaa agacattgtt tccgaaaagg aatttgtaga gtttggtcct
      601 caacacgaag cgttgagtgt agccaaatac cgtgaattgg ttgagaagaa aatcaatgaa
      661 ttggtttcaa gcagcccgat tttacaaaaa ttaaaacaag gacaagaaat cacaacggat
      721 gaagccgagc aattggcaga agaactgcac aacgagcatc cacatattac cattgatttg
      781 ctccgtaagg tttacaatca ccgcaaagcc gcattggtgc aattcattaa acacatttta
      841 ggcattgaac agttggaaac ctttgccgaa actgttacca aagcatttga tgattttgtt
      901 tccaaacaca gttacctcac cagccgtcaa attcaatttc ttgatttgct caagaatttt
      961 gtattggaaa aaggtgatgt aagcaaacgc aacctcattg aatctccttt taccatgctt
     1021 caccccgaag gcatcagagg cattttcaat caaaaagaga ttgacgaaat tttaagttta
     1081 accgaaaaag tattagcagc atagatgtta caaaacaacg cacaattaaa atcgcttata
     1141 gacaaactct ggcaaaactt ttgggaaggc ggcattgcca accccttaac cgctattgag
     1201 caaattacct acctcatttt catgaagcgt ttggatgatt tggaagccaa acgggaacgg
     1261 gatgcagagt ttacaggcga aaaatatgtt tcccgatttg ctggcaaatt caatattccg
     1321 ggaagtaatg aaagtattga taaaaacgaa ttgcgttgga gtgtttttaa acacaaaccc
     1381 gcagacgaaa tgctgatgca tgtgcaaatg aaagtgtttc catttttgaa agaactaaac
     1441 ggcgaaacat cacctttcac caaacacatg gccaacgctg tgttcattat gcccaaagcc
     1501 aatttgctgg tttctgccat caacattgtg gaggacattt tcaaagaaat tgaaaaagat
     1561 gccaccgaag gcggacatgc ttttcaagac atacaaggtg atgtttatga aatgctgttg
     1621 agtgaaattg ccaccgcagg taaaaatgga cagttccgca ctccccgaca cattattaaa
     1681 ctgatggctg aattggtggc tccgcaattg ggacaacgca ttgccgaccc tgcttgtgga
     1741 acaggcggtt ttttgttggg tgcttatcaa tacattctta ccgatttagt tcgcaagaaa
     1801 gaccctgcca aactgggcaa agacgaagac ggttttgaac gtgccgccat cagcgccgta
     1861 cttacccaaa aagtaaaaac cattttagac cagagttttg tgggttatga tattgacacc
     1921 acgatggtgc gtttgggttt gatgaatatg atgatgcacg gcattgatga acctaaaatt
     1981 gactacaaag acacgctcag caaaggctac aatgaagaca gccggtttga tattgtatta
     2041 gccaatccgc cctttacagg aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnngtaagca ccgccatttt gatttttacc aaaggtggtg aaaccaacca tgtgtggttt
     2221 tatgacatgc aggcagacgg ctatacctta gacgataagc gaaataaaat tgctgaaagc
     2281 gatttgcccg acattgtgca acgctacaaa acaagagacg ccaaaaaaga cagtgaccga
     2341 aagttgaaat actttatggt gccgaaaaag gaaattgtag agaacaacta cgatctgaac
     2401 ctgagtacct acaaagaaga agtgtatgaa gaagtgagtt atgaaaaacc gaatgtgatt
     2461 tttagcaaat tggaaagcat ggaagccgat attcaaaaag gattagcgga attaaaagag
     2521 attgtgtggt aaaatcccga agggatgaaa tgataataaa aaaaatgata aacaataaaa
     2581 tcaaaacccc gaaggggtga cataaatggc aggaacctat tcacaaatat atttacaagc
     2641 cgtttttgct gtaaaatgca gagaaaacct catcgccaaa tcatggaaag acgagttgca
     2701 taagtacatt tcaggcatta tcaaagaaaa ggaacaaaaa ccaattatcg taaacggaat
     2761 gcccgaccac attcatgcat tcataggttt aaagccatca atgagaattt cggatttggt
     2821 acgtgacata aaaaacaatt catcaaaatt cattaacgaa aaaggctttg taaggggcaa
     2881 gttttcctgg caggaaggtt atggtgtgtt ttcctattcc cattcgcata ttgaaagggt
     2941 gtataactac atactgaatc aggaagaaca ccaccgtaaa aagacgttca aagatgaata
     3001 tctgggattg ttaagaaagt ttgaaattga tttcaaagag gagtatgtat ttgagtttat
     3061 ctaaatatat caccccttcg gggttgggta gttgggttag tctggtgtgt ttcaatcatg
     3121 ccaacccttc gggtttatta tcaataagca tggaagctga tattcaaaaa gaattggcgg
     3181 aattaaaaga gtttgtgtga tgatgtttga gaaacttgga gatattgcta caattaaaac
     3241 gggcaagtat gatgtaaacc attctactcc tgatggagaa tacgtattct atacttgtgc
     3301 atttggccaa ttcaaatcac cgacttactc ttttgaaggc cctgcaatta tattacccgg
     3361 gaatggggca aatgttggtg aagttttcta ttacgatgga aaatttgaag catatcaaag
     3421 gacttatgtg gttcaaaatt tcaaagctga ccccaattac atctatgcct attttaaagc
     3481 acagtggaaa aacagtttaa gaaataagca gtttggttct gctacaaact acattagact
     3541 aaataatctt acggatttca aaattcccct tcccccactt cccgaccaac tccacatcgc
     3601 caatctctta agcaaagccg aaaacctgat agcccaacgc aaagaaagca tccgcctgct
     3661 ggatgaattt ttgaaaagca cgtttttgga gatgtttggg gatccttctt ccaacagaaa
     3721 gcaatttcca aaaggctcaa taagggatgt tgttgccgaa gtaaaatatg gaacaagtag
     3781 tcctgcggaa gacgaaggat tatatccgta tctaaggatg aacaatataa ctcctgatgg
     3841 ttatatggat tattcaaagc tgaaatacat caacattgtt gatgaaaagg agaaagaaaa
     3901 atatgttgtg agaaaaggag atttactttt caatcgaaca aattctaaag agttagttgg
     3961 aaaaactggt gtcttcaatg aagattcaga aatgattatc gctggttatt taattcgtgt
     4021 taggactaat gaaaaagcaa atccttggtt cctatggggc tatttgaatt caattcatgg
     4081 caaacaaaca ctacttggaa tgtgtaaaag cattgtcggt atggcgaaca ttaatgcaca
     4141 agaattacaa ggaataaaga ttctgattcc ccaaattgaa ctccaaaccc aatttgccca
     4201 aatcgttaaa aaaaccgaag ccctcaaaac ccaataccaa cagagtttgc aggagttaga
     4261 aaatttgtat ggcagtttaa gccgaaaggc gtttaggggg gagttgggtg taaaggaaat
     4321 ggcataataa aaaatacgcc accgcacaga ctaacacaac aaagacaatg gtttgcaatg
     4381 gttgaccgcc agaaggtcta ctgataacat gcgtttggcg tcacgcgaag tgaagtgctt
     4441 aaattcaagt tcaatttttc aaatcaactt tagcgctggt tgatcccgca ccaataccaa
     4501 actgagaatc cttaacctta actatcaaac caccgccatg cccatcgaat atagttttga
     4561 caaaagaccc tccgctgacc agatcatagc gctctatgat agatcgggtc tgccgagacc
     4621 gacccaggac aaagaacgca tacaaaagat gtatgaccat tccgatctga tcgtaaccgc
     4681 ctgggacggc gacctgctgg tcggggtttc ccgctcgatc acggattggg tttggagttg
     4741 ttacctcgcc gacctggcga tccatcccga ttaccaaaaa aacggcatcg gaaaggaact
     4801 ggttcgcctc accaaagaaa aagtgggcga agaagtcatg atacttttgc tttcggttcc
     4861 caccgcgatg acctattatc ccaaaatcgg aatgaccaaa gaagaccggg cctttaccat
     4921 tctccggact aaataaacac atttcaccaa cagtgatgaa tggaaggtca ccgcatgccg
     4981 tacgacttac catacacgaa atcaaaaaaa acttaaacaa agtgattgat gaaaaacccg
     5041 tttgaaatag aaaaaacata catcaccgaa tattccccaa gccgtatcac ggccatcgtg
     5101 caagaaacct tagaaaaaaa gaaccgtatg gtgttctttt cattaaaaac ttttaccggc
     5161 gcgatcaaag aaaaccattt ccgcttcaaa gaaaatgtat gggccaccaa taacggaatg
     5221 atctatccga ccatccaagg cacgatactg ggtgataacc cgacaaagat acatatccat
     5281 attactccgc gatacgggtg gatattttca ttcgcgctgt gcgctttagt ttttttatgt
     5341 gctgcttttt ttacggatcg catgtccgtt gacggtatcg aaagcgcacc aacgctgtat
     5401 gaacgatcgg cttttgcaat ttttggcgca ggtatcccaa tgcttctgtg ttacgttacc
     5461 tgcttaagac ccatccaaca tgctgaacga tggattataa aaaaactcga tctcaaagaa
     5521 atcacttcat aaaatgaagt attacccgcc gcatcacgta aaaataattt accctaaaac
     5581 gccggtacac acatgccggc tttttatttt actttttttt cttacattga ccgggatgcg
     5641 tgcccgataa tcttatgatc gaattaccta tactcaaact tatgccggct atagccaaat
     5701 attttatccg atgcgctttg gtatttctcc tgctttcgat cctgatacag acgttgacgc
     5761 aagtgggacc gcattggggt tggacgacca ttcacaatct tcagcccgta ttttttcatt
     5821 ttttcttatt cggctgggtc atgcagatga tcatcggtgt atcgctatgg atgtttccgt
     5881 ggcaagataa ggcgcagttc atactccgcg accgggtcgg ttggtttgca cttttgacga
     5941 tcaatagcgg cctcgtgctg cgcggggtgt gtgaaccgtt tctcgatcca tcctccggcg
     6001 atgcgttgcg tttcgcgctt acggcttccg tgatactgat gtggctggga tgcgtcgtat
     6061 atgccgcgca tatctggcaa agagtgcgga gcaagtgata tggatcgtct cagtatatgg
     6121 tggattcgcg gcgcgttgat ttattttgta gtcggcgtta cgctcggcgc acttttactt
     6181 ttgcacaaaa cctggtcatg ggatatgctt tggtggcgtt tgctgccgct gcacatctat
     6241 accatgttgc tcggttttat gatacctttt atcatcggcg tggcgcactg gatactgccg
     6301 cgcaacgacg gcccccacga tcgcggcaat cccaaactcg ttatcaccgc ctggatactt
     6361 tggcacaccg gcctcatcgc agggctcact tccgagatta ccggatcggc gctgtccgcg
     6421 atcacattga gcgtggctct gtgcaatatc gcagccgtta tgatcatgtt cactcaacta
     6481 tggcaacgtc ttcgattatt caaacaaatc tataacggca aatgatccca ctgcatcaaa
     6541 atcttagaga tattttgatg agaatgcgac gctgaaaaaa actgcttttc ccaaaacctt
     6601 gtgagatctg aagtggcaca aaaaacggcc attgtattac tgcagcttgg cggaccggat
     6661 aacttagatg cggtcgaacc gtttctttat aatttatttt gcgatcccga tattattgat
     6721 ctgccgggcg cttttctttt tcgtaaaggc ctcgcacgtt tgatttcctc ccgccgttca
     6781 ccggccgttc gggaactcta caaaaatatc ggaggccgtt caccgatctt gccgcaaacc
     6841 gaagagcagg cacaggcgtt gcaattacga ttggatacat ccggtcacca atgtaccgta
     6901 tttatcgcga tgcgctattg gcatcccatg accgatgcga tcgcccgatc catagcccaa
     6961 ggcggctatg atcgtattct tttgcttccg ctgtatccgc attattgcaa agccacgacc
     7021 ggttccggta tcaacgaatg gaggcgctgt gcaaaaaagt atcaaattga tcacatccca
     7081 acccaaactg tcgcaagtta ttttgatcat ccggattata tacacgctat cgtagataac
     7141 ataaaaaaag cgctgaccca attgccggaa gatgcgcgcg cgaagaccca tttggtgttt
     7201 agcgcacacg gtacgccgat gaaattggtt cgtgacggcg acccgtattc aatacatatt
     7261 cgtcgcacct acgaagcggt gttatccgcc ggtaattttg gtttaccgca tcatttgtgc
     7321 ttccaaagca aagtcggccc tcagcaatgg cttgaacctt cgctggtaca aaccatcgct
     7381 gatctgtcag ctcaaaaagt atcacacatg ctggtcgttc ccgtcgcgtt tgtgaccgaa
     7441 catatcgaaa cgctttccga aatcaatatc gaagtgcgtc atgaagcgga acaagcaggc
     7501 gttcggtatt ttgatatgat gcctgcactc cgcgatcatc catcttttac cgcgtgtctc
     7561 tacgacctca ctctaaaggc attgcacggt gcttggcccg tatcgcaatc ataggcggct
     7621 gatcatatag taatgttcct aagtagatat tgaaaaataa ctccttcggg gcgatatacc
     7681 tttaacaaag aaactttttt aaaaaatacc gtatagatta gaaaccataa ccgatacttc
     7741 gccatgcgtt acgttgatcg ttttcccgat ccgcactccc gccgatttga taaagccgaa
     7801 tggcgcgatg aacatggtac ggccaacgtg atcattcatt gccaatcttc aggaattgct
     7861 tttcccaagc attggggtcc gctctcgctc aaaaccacat tatccggtca tgaatactat
     7921 gtcacacggc atagcacgca ccgcgttacg ccggaacggc ttttgatact caatgaagat
     7981 acgacttacg ccagttatat tcgcgacggt gaaaccgccg aatcgtttac gatcaatttc
     8041 agtgcggatt tcatacgcac cgttttgcgc gatagcctat ccgacggtat gcaaaaattg
     8101 gataatcctt tttccgacga tgcgccttcc gtacaatttc cggagtacac gctgccgtat
     8161 caccatgcta tccgggtccg tctgaaccgt attcgacgat gccttaccat gcccgcggat
     8221 ttgaatgggc gcgtggacga agagctgtct tttttatatg aagcgatgtg cacgctttcc
     8281 gtatcagaaa acaaaaaaat acgcgacctg cctttccaaa aagcctcgac tcgcgaggaa
     8341 atgtaccggc ggcttcagcg tgttcgggat tatatggatg cctgttatgc ggaacctata
     8401 aacctgcacg atctcagtcg cgtagcgtgt ctttccgtgc atcatttgat tcgccttttc
     8461 aaatcgcatt ttttatgtac gccgcatcaa tatctcattc gatgccgatt gctcgaagcc
     8521 aaacgactca ccgaaaccac ttcccttcgt attgaagaca tcgcacgccg tgtcggcttt
     8581 gaagaccgaa gttcattttc acgattgttt cgtcaccatt tcgacaaaaa cccctccgat
     8641 cgcacacaat aaaaaaagca attttcacca ccttgttttg acgaaccgct gcgcatatta
     8701 tagggatgat actattccta aacgaaaggc acatgtatgt tgatgcgatc ttttattgtc
     8761 tcgctttttt ttatatggac taaatccttt gcacaaatgc cgatggattc ttccgctttt
     8821 gatttttggg tgggcgaatg gacggcgact tggtccaacg ccgacggcag caccggtaaa
     8881 ggtaaaaatc gcatcgtcaa aattttagac ggcaaagtga ttcaggaaca ttttgaagac
     8941 gaaagcggat tcaaaggaac aagcatttcc gtatatacgc cggctcaaaa aaaatggcga
     9001 caagcatggg ccgataacca aggcggttat tttgattttg aaggcgacat atcggacgga
     9061 aaaaagattt ttaaaaccaa aatgcacgaa gccaacggca aacaatatat gtcacgtatg
     9121 ctgttttacg atatcaagac ggatgcgttt acgtgggatt gggaaaaaac agaggacggc
     9181 ggcaaaacat gggccttgca atggcgcata caatacgcgc gtaaaaaata atggaacgac
     9241 ggattgaaat ttagttcgcc cccgtcggca catcataata tttacttttg atccacaacg
     9301 cgacgcgaac gaggcttacc aaaacgggca cctcgaccaa aggaccgatc acggcggcaa
     9361 acgctgcacc ggatgagatg ccgaaaaccg ctatggcaac ggcgatcgcc agttcaaaat
     9421 tattgctcgc cgcggtaaac gaaagcgtag ccgacttggc atagtccgca ccgctgcgac
     9481 ggctgagata aaaagaaatc aaaaacatca cgacaaaata gatcaccagc ggaacggtga
     9541 tgcgcaaaac atcgaacgga atcgttacga tatactcacc tttgagcgaa aacataacca
     9601 cgatcgtaaa gagcaacgcg atgaatgtca gcggcgatat tttgggaata aacttttcat
     9661 gataccaaac ccggcctttg gcttttaata aaatcatccg cgtaagcatc ccggccaaaa
     9721 aaggaattcc gagatagata aatacgcttt cggcgatctg catgatcgtg atatccaccg
     9781 caaacgcttg taatccgagc aaggagggaa acaacgtcaa aaagagatac gcatacaccg
     9841 ggaaaaacaa aacctgaaaa atagaattaa aagcaaccag acccgccgcg tactccgtat
     9901 caccatgcgc aagttcattc cacacgataa ccatagcgat acagcgtgca agtccgatca
     9961 tgaccaaacc cgccatatat tccggataat cgcggagaaa tacgaccgcc agcaaaaaca
    10021 taaacacggg gccgattaac cagttttgaa cgagcgacag aagcaaaact tttttattcc
    10081 gaaaaacatc cgcgagttct tcgtatttaa ctttggccag cggcggatac atcatcagaa
    10141 tcaaccctat ggcgatagga atattagtca taccggattg gaaccggttc cagaatccga
    10201 caatggacga aaaaaaataa cctgaaaaaa cgccgatcag catggcgata aaaatccaca
    10261 acgtcagata ccggtctata aatgaaagtt tgctgacttt ttccatactc aatcttgcgc
    10321 tttacaccac ttatcgaagt gatttttgat aaaattcaaa aaagtctttt ttaatttcat
    10381 cacggatgcg gcgaaattct ttcatcacat gttcttctgc cccagaagcc tccgccggat
    10441 catcaaaccc aatgtgcaat cggtggctca cagttcccgt gaaaacaggg cactgttctt
    10501 gggcattaac gcatacggtg atgacatagt caaacggctt atccaaaaaa agatcggtgc
    10561 ttttgggata gttaccgctg agatcaattc cgatctcgcc cattaccttg acggccttcg
    10621 gatgcacttg agatgaaggc cgggtacctg cggaatacac ctcaagcgaa gcatcaaacg
    10681 atttaagaaa cccttcggcc atttgactgc ggcaggaatt cccggtacaa aggattaaga
    10741 cacgtttttt cataaacctc acctatagat attaattcgc taaaaaacga agtattaaga
    10801 acacgtgatg actttggatg attgtatttt ttttacatcc gactgtacct gcgccgtatc
    10861 gttgagcgcc gtgtgaagta atatacgcaa cgcggttatc tccggataca gaggttttgt
    10921 attcaaactg taatataccc agcggccctt tttctcttcc gttatatagg cgtttttttt
    10981 caaaattttc aaatgctgcg aaacggtgga tggcgccaac tttaagacgt cccgtatttc
    11041 acatacgcac aacgtacgtt gttccagcat tttgagtatg cggagcctgt taggatcgga
    11101 aagcgctttg aatacttcgg cgtaattttg aatagtcata ttaattcgtt tattgacgaa
    11161 ttataataaa caatcgcata ccaagataca attcgttttt attgtaaaca caccttaggt
    11221 ataaatgcgc attatagaag ttatgcttga caatgcgata cgaactattc taatttgcgc
    11281 catgcaacgg atttttaaat cttcattgtt tttcactgtt ttcgttttat taagcatggc
    11341 ttcgcatgcg tattcggcca gccaacaaaa cgaaaccggt ttcccgcata aaacgacaca
    11401 agagccgacc tagtcatcat tggaccagcg tgacggttta ttttttcttt catcatcccg
    11461 cgaagaaatc gcatcccaat taaaagaaac tcgtcataaa gcgtggattc tgccggcatt
    11521 gtccgtttct ttcattacgt accgcaaata cctcgatttt tttcaactct cactgactcg
    11581 caattttctt ctcggatata cgacaggtct tcgcgccccg ccgcgttgct gacatctccc
    11641 cctctataaa tcatttatcg gccacccggt gtggctgaac cggcacagct tcatgccatc
    11701 cgtggtggtg tttctcacgg aggtactatg agtttttcaa ccgatgtgct caccaagatc
    11761 gttgtgatcg ccctatcgat cacgatcttc ggggtgtttg tacatcttta tatcaagtcc
    11821 ggaaaatctg aaaaaaactg acatcaaacg gagtatatct atgtcaatct ctcaacccga
    11881 atcgcgccct ttgatcaaca ttacggaatt attcaattcc gatccggaat gggccgcgaa
    11941 gcgtgataaa gctattaaga aactgtacga cggtaatacg caggaattca acgcgtttat
    12001 gtccaaactg gagcccatgc gcgactggaa agacgtgatg gacgctgttg aagccgagtt
    12061 tatgcgtaaa aagatcaaac aagacagcaa agaagcaacc ggtcttacgg atgtgctttt
    12121 caaacggtac ttcccctcgt attagtccgt ctggatatat tcatttctta ctccgagctg
    12181 cgcgaataat ttgcgcagct cttttgtttt atataacctc tctttgccga aaaaatcttg
    12241 actattcacg gcttaatttt tatgttgaac cacgataacg acggatttgt tctcactata
    12301 aaaaattttc agaggagtag atatggctta tattattgca gaaccgtgca tcggtgttaa
    12361 agatacagcc tgcgtcaagg tttgcccggt ggactgcatt tatgacggcg acggttggga
    12421 tcagttgtac attcatccgg atgaatgcat tgactgtggc gcatgtgaac ccgaatgtcc
    12481 tgtcgaagcg attttcgccg ccgaaaacac gcccgataaa tggaaaagtt ttattcagaa
    12541 gaacgcggat caattcaaac aatagatccc ttcttgatta cacacgaaaa ggcttggact
    12601 tgttccaagc ctttttattt tttactctcg tcgttgccgt agaaccgcac ctgccgtgca
    12661 aaaaaaatgg ggaaatcaaa atgaaaaaaa tcacgttacc ggcgtttatc tttccttgcg
    12721 ctttgattct gctcgattat gtaaccaata aatcaaccgt tacttttaat accaaccgta
    12781 tcctggcgta taccaacgga tttatcggtt ctattgcgtt ttggactttt tatcaattag
    12841 cttcggcgta cacgctgcat cattatcgcg aacacaaacg ggttcgcatg cttttcattg
    12901 tcgggcaaat tctttttgcc ttggtattta ccgctacgat ttcggtaagc ctcggatatt
    12961 attatgagtt tggtaacttg cccaataagt ttgccgtggc gtatctgatg gatgaatgga
    13021 ctgacgcggt gagtcttatt accggttata tttctatgcc gctgatgttt cttctgattt
    13081 ccgtattcgc agcaatatat gcctgccact attttgtgat acgtcatatt gcacaacgta
    13141 tgcacacgag taccccggct tcggtgaaga aagtcttagc gattttggcc atcatcaccg
    13201 cggcatcgtt gctcggaggt tttttttcca aaaaactgtc cgctacgtta ccgatggact
    13261 tcgggagttt acaagccgtt gcaaaatatg cgttcggcga taagtcggga aaaggctatt
    13321 ccgttcccca gcgaattatt ttaccggaaa atcggaccgc ccgaaaacca tccaatgtat
    13381 tgttgatcat caacgaaacg ttgcgcgccg attttatcgg ctatcagaca ttggccaatc
    13441 cgattcctcc ggcttcacgc atgaccgacg caatcgcttc cgacagcttg tctttcgtat
    13501 tcaaacgcgc acgcagcaat tcgagttcta cccgtgtcag tttgcccgct attctcatgg
    13561 gcatgattcc cacaaccgcc acttccgccg attaccatcg catgccgacg ctttggaatg
    13621 tgtctaaagc gctgggttac cgcacctttt ttgtcacgcc gcaaaattgg gattggcaaa
    13681 atctagacgt attttttctc aataagcagg tggatcaaag tataacggct aaagatatcg
    13741 gcccgcccta cgtcaataat gcgggtgtgg aggatcaccg catattggat tacactcatt
    13801 ctatttttca ggaatttaag aaaaacaaaa atccgttttt tggcgttatt cagtttaact
    13861 gcacacacca gccgtataat ccgggcgacc ctcatacggc gttatcacct aactcaaccg
    13921 agcggtatgc cgctgcggta cgatatttgg acagtttgat tgcacagata tacattcaac
    13981 tcaatgaagc cgggctgaaa gacgaaacgc tgatcatcgt gacaagcgat catggtgaaa
    14041 attttaaaca tcgcggtctt gctcgattga ataatcttta cgacgaagtc atacgcattc
    14101 cattatgggt aagttatccg cgcaaatatt atcaaactcc cgagggcaag gcgcgatttc
    14161 acgcctggaa acaaaatgaa tatagcgctg ttcaaaatgc cgatattttc ccgaccttgt
    14221 taagttactg gggtgttgac aaaacctcac ttcagggtta ttcttatcag ggaatgcgat
    14281 gggaagatcc ggcggattct ctgcgtatcc tgagcggtac aaataccggc gatatccgaa
    14341 agtggaaccg tgaagagttt tttttattac gccaccatgt gaagtttttg gcaacggata
    14401 aggcagggcc gcagatttat aatttgcaat ccgacccgta tgaagaacac aacttgtggg
    14461 acgatttatc ttttagagaa gcgcatcggc catggataca tcaatacata tccgaccatg
    14521 cggtgctcgc agaattggcc aaacgaatga aaattaattt gggaaacgga gccgccatac
    14581 acccatcgga tacttcgttt tcgcgtgcgg atattgattt gaaataatac gaaaagttta
    14641 tatatatagc cgattcattc aacaatgtcc catttgctga agaggtaatc atgaaaatag
    14701 ctattggttg tgatcacggc gggtttcccc tcaaagcgac ggtgatcgaa actgttcaga
    14761 aggccggtca cgaaaccatg gatctgggaa cattttcatc ggatcctgcg gattatcccg
    14821 attattcgga aaaagtagcc caggcggtac aaacgggtct cgccgatcgc ggtatcgtca
    14881 tctgcggcag cggtgtcggc gctacggtgg cggccaataa attcaaaggc attcgcgccg
    14941 gattatgtca tgatacattt tcggcccggc aaggcgtaga ggatgacgac atgaatgtgc
    15001 tcaccctcgg tgcgcgtatt atcggtccgc ttttggccat cgaagtaatc caagcatttc
    15061 tcaaagcgcg cttttccaac gccgaacgtc acctccggcg gttgaataaa gtcaaaggtt
    15121 ttgaagaaaa tttcggcaaa taaaaattct tatttaattt tttaactact ggtcctatgg
    15181 aaaaacctta tccgccggtt tattacgccg actatttgga actcaatcaa ctcctcggcg
    15241 cacaacatcc taaaagcgac gcgtatggca aaccggcgca cgatgagatg ctgtttatca
    15301 tcattcatca ggcatacgaa ctttggttca aacaaatttt gcacgaagta gattccgttg
    15361 cacgcctgtt tgcagaagat catattgatg aaaaaaacat
//
LOCUS       DAOQGF010000081        15253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1791_length_15253_cov_7.439334, whole genome shotgun sequence.
ACCESSION   DAOQGF010000081 DAOQGF010000000
VERSION     DAOQGF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_15253_cov_7.439334"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..2032)
                     /gene="pepF"
                     /locus_tag="PK937_11155"
     CDS             complement(146..2032)
                     /gene="pepF"
                     /locus_tag="PK937_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNH33173.1"
                     /translation="MHTMEVLFIFTIGLIAGTVAFAQERDRTKIEEKYKWNLTDIYKS
                     DDDWKKSKDQLILDIQKLAPHKGRLGQSAQHLLDGLHTMFDLSKTFTRLYSYASMSSD
                     QDTRDSKYLAMVQEMSQIGSSFSAAASFIEPEILALDRAMVDGFIKQEPKLAIYKFYL
                     NDILRRQAHTGSAAEEKIIADASLMADGAQNIYSIFSNADFPYPEATFSDGKTVKLDQ
                     ASFGFFRASQNREDRKKTFSTFFGKLNEFRRTYGTQLYAEIKKNIFYKNARKYNSSLE
                     SALDANNIPTDVYHNHVKNVNANLNSFHRYLKLRQRILGVDQLHYYDLYAPLLKNVDL
                     KYSVDEAGKHITTSLAPLGKEYVGVIEKALNERWIDMYPTDGKRSGAYSNGSAYDVHP
                     YMLMNYKGKYDDMSTLTHELGHTMQSYLSNKKQPYPTANYPIFVAEVASTFNEALLIE
                     YMLKNIKDDETRLSLLGSYLEGIKGTVFRQTQFAEFELRIHEIAEKGEALTGDRFNEI
                     YMEITRKYYGHDLGVCIVDDDIKIEWAYIPHFYYNFYVYQYATSFTASAALSEKVISG
                     DKTATQAYLNFLSSGGSKYPIELLKDAGVDMLSTQPIELTMKKMNRVMDEMEKILDRM
                     NKKK"
     gene            complement(2112..3185)
                     /locus_tag="PK937_11160"
     CDS             complement(2112..3185)
                     /locus_tag="PK937_11160"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNH33174.1"
                     /translation="MQLDIIRVDENKKKIKPTDESTLGFGKIFSDHFFHMTHTTKLGW
                     HKAMIEPYRALSLDPAAVVFHYAQEIFEGLKAYRGKDNGVYLFRSKDNFRRMNRSAVR
                     VCMPELDIEFVNDALKKLVLIDQDWIPRSRGTAMYIRPTMIATEPSVSVKSSDEFLFY
                     ILLGPAGNYYPEGFNPIKIYVSKKHIRAAKGGLGEAKTGANYAASLYTAQEAKKKGFT
                     QVLWLDAFEHRYIEEVGAMNIFFRFKDEVVTSPLGGTILPGITRDSIIQLLKHWGMKI
                     SERPITIDEVAEGAQNGNLLEAFGAGTAAVISPVGEFSHNDQPIIVGQNKVGELSKKL
                     YDELLAIQYGEKPEPFGWVERIG"
     gene            3626..4495
                     /locus_tag="PK937_11165"
     CDS             3626..4495
                     /locus_tag="PK937_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33175.1"
                     /translation="MVARSIVVKWKTMKPGILGFASFALCLTFLVVSCEKPETNVGTN
                     NPGDEQVITVSSKVISTDPKISNSGDLPHYSQSLGVYYTKYYNVGNTRESMSALLIHR
                     IGNTFVSYGNSISLGGKVINRSMDSSSSQGSTYFFYGVDSDIPEGLKLATPPQFVVSN
                     SDQINNISKTLTTKVPIAATNIALGTHIDVSNDFTVQFNRVLEKGGVSINASGYIRTF
                     ELTSPADHLTIPKEELQAIIGENATRGVVVWIWDNSVVDTITSTDKNTNAEYSLPVVE
                     FTAVYVDDVHLDK"
     gene            4583..6790
                     /locus_tag="PK937_11170"
     CDS             4583..6790
                     /locus_tag="PK937_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH33176.1"
                     /translation="MNKLKTAIVFFMFVSACQLFAVDSLTYVRITAPEIESMGGWPMA
                     RKWYGTMINHLRAHGAQQIFVDISFLHADALHPESDAFFYSVVSRESDVYFLNADTSA
                     DTFSFLGIHRLPKAHSFGLFSDFFEISADRMIYHPSQRSLTSILAGPSGPERSVVIEY
                     PDTAIVPEISWLSVIHNTIDRPLHRVVVALDYPGTTSYLYRPDGVAVSTSYTQIWAAH
                     CLIQGHYRYRLSTGVFVMMLILVIGTVIALISWRTSVSVYVTLVSGGILIALVGLMGY
                     AINGWFYLWIMPGLLIGSYRLWQQIMRTNIKPPVAVAVPDKDVNESDYALLKYKLNFY
                     ENAAQTLPDDVLQKSDNESGVLFHETSPLREVLVKAAGVASSDIPVIVQGESGTGKER
                     LAQFIHARSARSEFPFIAVNCGSFNENLIEAELFGYEKGAFTGAHQSKPGRFELANKG
                     TLFLDEIADTSLAFQVKLLRVLQEGVLERVGGIKPIAVSVRLIVATHRDLRKMVREGG
                     FREDLFYRLHGFEITLPSLRERRMDIEVLVASFLKEANARLTFSDELMSWLCQQNWPG
                     NVRQLQSATRRAIVNAGLRGRTFLLPEDFELGTIEKLMPNEDTAERILSAFRKNEFRH
                     RSVSAVASDLSIHRVTVTEYLRGWILRGMSKKNDEEAIMNFLTGGAVVYDVEPLRRRI
                     SEYKSYIESRIDEGLRNNDRPENFVALRYKNLPGEFHEDAEAVYRDYLTNSKL"
     gene            6787..8790
                     /locus_tag="PK937_11175"
     CDS             6787..8790
                     /locus_tag="PK937_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33177.1"
                     /translation="MRRTNIIYQLAILNALVFAPAKHLAAQSSFLFGNVDSIYTNIRF
                     TGHSTQIAGSGIYYANSDIGISHRRSGWLWDVVARINQAQSYLIDPGHVVIQLPDTVT
                     LRENGDLRSRAHRYTWNNPFSLIAGRQWNKWYIGTEHQLVSRQEVLKEPILREIGNNG
                     RTYYLEWKTSSRTRYDIWSAAHIGYRWNNLTVDIGTTLIPIVGIGKMPKQFDQQTTLR
                     WRLGLAAQHWGGYSRILWSPDVWSASRTQLWTSRIMTGISMTGERILSPRHIMRVDAG
                     YLHRDRWAFYVAVSSSVEKNESLKRASFEDMLRHIHHGSEGSMTPRAMRPRISAGIVY
                     RLDKTKPPVILRVTRSELLQSNIFASKNSYYAYNPVAFIELYNPETVPVDGQIIAEIE
                     DIGIRFQSEALRWQPGEIKRVPIFLYFDPAIFGEGHLAKQLRIGFEKDTYRYQLSAFP
                     VTIHDRHTWDGNTWGLRYFLTPENPIIRSAAKQLYFSEPDTVVTSDLIKFVRLRNLLE
                     KAGKNLRYIEDPVTTIYADQVQYPEETLVKQSGDCEDISVYIASLCMSVGYACGIVDI
                     RPDQNAGVYPSASGRSSPGHVFLLIDTGLSPEWAERAGFSEFDAIARDNTKGETSLWV
                     PIEATLLHEGFIRAHRDGVRQYYDKVIVQQGVSKGSVHIYDLQ"
     gene            8777..9472
                     /locus_tag="PK937_11180"
     CDS             8777..9472
                     /locus_tag="PK937_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33178.1"
                     /translation="MIYNKIYRLIFLTSMVACALYAQDKESWIQKTSGEALYLEPRTG
                     QWIPISAKQTIPLRTYLITHHGSKTTLYDQAEMLVLPDGAYFFVEDAIPKDRDALVGA
                     LTQIEAEHLPNRLESRPVSVGLTYGAPEHKNEEQIPYLAERINAVNRFYELGRKDASL
                     LSLKRLLSKYPRLYRDKKNVEQLFSLYENLELYGYLYDESSRLLNLSQDAEWSVFING
                     WYKTARTYVTQQH"
     gene            complement(9606..11999)
                     /locus_tag="PK937_11185"
     CDS             complement(9606..11999)
                     /locus_tag="PK937_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33179.1"
                     /translation="MLSNYLTVAWRSITRKKGFSAILISGLAVGMACFLLIALYALDE
                     WSYDRFHGKKDRIYRLAEKLESENSSSNPFPVAQALKTDYPHLVEQTVRLFNFQLTSF
                     TLQIGDQKYNESNLYFADSTFFQVFDYALAEGDPQTALARPNSIIVSRAMAKKYFGDT
                     PPLGKILRLEGKLDLMVSGIFAEIPTQSHIRFDALISFSTAASLTGPNTFKGWVWNPC
                     WTYLLLKEGVSPDELTAQFPEFIKKYYPNFLKAQVTHYLQPLTDIHLHSKLDYEMHPN
                     GDAATVWVFCTVGLLILLISCINFTNLATARSLMRAREVGMRKVLGADRKMIVRQFLG
                     ESLLISLIAWVGALILSEISLPLLNALTGKNFSWAAFQYAWYIPLGLLVAALCGVLGG
                     WYPSLFLARFDAVTVLKGQFKHSGKTRWLRQTLVVMQFAISTGLIAGTIVVYLQLHYM
                     RSADTGFQKEHILVIPTRPAMIKRYETLKSEWKSDSRIQDVTVMNEILGVHHNTHEIR
                     YEPMPVGQWQYFPGLMVDEDFAETFGLQLLAGRDFSKDFPRDDSLGILVNEAAVERMG
                     WGSPEKAIGQIFETIGGHERVIGVVKNFNYKSLAHRIEPFFIDMPNAGNFNFFRKFMA
                     LRIRAHDLPGAIAHIESVWNRIVPEYPCEFSFLDQNIEKMYRAQENLSRIIGYFAGLA
                     IFIACLGMFALASFMAEQRTKEIGIRKTLGARTSELALLFARDFVILVLIAAAVSSLP
                     SAWFMQDWLNNYNFRITLEWWIFASSAGVALLIAALTVSYQAVRTASRNPVKALRYE"
     gene            12216..14288
                     /locus_tag="PK937_11190"
     CDS             12216..14288
                     /locus_tag="PK937_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH33180.1"
                     /translation="MASKAQTRPDMVRPGNGKKYSLSDFPELTKERLLAAYRHMIIAR
                     YLDDKMLRMLKQGKSFFHIGCSGHEAAQVAAGFAMQPGKDWFYPYYRDQALCLSVGMT
                     AQELMLCFLAKADDPNSGGRQMPQHYGHAGLRIPSQSSPTGTQFLQAVGCAMGAAKEG
                     KDEVVYVSSGDGTTSQGDFHEALNWASREKFPVVFFIQDNKYAISVPIHQQTSDSILS
                     LGAGYSNIHCYEMDGTNFLESYAIIKEATEKARKGEGPALVVADVVRLLPHSSSDDQR
                     KYRPEEDLAKDRARDPIEQMAKFLIDNRILKKEDDEAFRQECYKSVDDAADWAETQPH
                     PDKSVAMKHVFSEEPDTLAYEKTVPSGKPIVLVDAINHAMAEELERDPRIIMFGEDIE
                     DDKGGVFTATKGLSKKFGRDRVFNSPLAESSIVGTAVGLAVRGWKPVVEIQFGDYVWT
                     AMMQIRNELATMRYRSNGTWKCPMVVRIPIGGYIHGALCHSQNIESFFAHIPGLKIAL
                     PSNAADAKGLLKTAIRCDDPVLFLEHKGMYRQGFAQMPEPDADYLLPFGKAAVKREGS
                     DITVVTYGMMVNKCLNAAKYMEETHNVKIEVIDIRTIVPLDTDTILNSVKKTGKCLVV
                     HEDVEFCGFGSEIVAQIMHQGFEYLDAPVQRVAGAFSPVPYNWFLEEMILPQDHNITT
                     ALENLAKY"
     gene            14483..>15253
                     /locus_tag="PK937_11195"
     CDS             14483..>15253
                     /locus_tag="PK937_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33181.1"
                     /translation="MRKVIFLCLVALLMQDQLSAQTSVGFSDNNPKPIKDYRLPDWGY
                     SRGWINASLSRNNSSSDYSDEEYKNDNSSYWLNSNYILMRESEKQDLNFSASAFYSKR
                     TNETSRNNDLGHSDRDYNDIVAHVMLRGNSSHYAGYYHATILPHIAYTKSESVYKVTD
                     IGYMFPDTAIMRSKEKSNAIDLSLATLFGIGRIRNVTPVIRAMRVHERLNAVGKGESW
                     NEETTQRVAAEFARLYGYQNVYDRHQKEFWNSLDEALPD"
BASE COUNT         4120 a         3205 c         3687 g         4241 t
ORIGIN      
        1 aagtgatatt atcgaatagc gtttttacac caaacgcttt accggcattt tcgacggaca
       61 gaagattcat acaaaaaact catttctgag aaaagaaaac gcctgagcgg cgaggcccag
      121 gcgtttcgtg gatgataggc gttttttatt ttttcttatt catgcgatca aggatttttt
      181 ccatttcatc catgacgcga ttcatcttct tcatcgtgag ttcgatcggt tgtgtgctga
      241 gcatatccac gccggcatct ttgagcagtt cgatcggata cttggaaccg cccgatgaga
      301 ggaaattcaa atacgcttgc gtcgctgttt tatctccgga aatcactttt tcggataatg
      361 cagcggaagc tgtaaatgat gtggcgtatt gatacacgta aaaattataa taaaaatgcg
      421 ggatgtaagc ccattcgatt ttgatgtcgt cgtctacgat acacacaccc aagtcgtgtc
      481 cgtaatattt acgggtgatt tccatgtaga tttcattgaa gcggtcgcct gtcagcgctt
      541 cgcccttttc cgcgatttcg tgaatgcgta attcgaattc ggcgaattgc gtttgacgaa
      601 atacggtgcc tttaatgcct tcgagatagc tgccgagtaa ggaaaggcgc gtctcatcat
      661 ccttgatatt tttgagcata tattcgatga gcaatgcctc attgaatgtc gaagcgactt
      721 cggcaacaaa aatcggataa ttggccgtgg gatatggttg ttttttgttg gacaaatagc
      781 tctgcatcgt gtggccgagt tcgtgcgtca gtgtgctcat gtcgtcgtat ttgcctttat
      841 aattcatcag catatacgga tgcacatcgt aggcggaacc gttggagtac gcgccggagc
      901 gtttgccgtc ggtcgggtac atatcaatcc agcgttcgtt taatgctttt tcgatcacgc
      961 cgacatactc tttgcccaaa ggcgctagcg acgtcgtaat atgtttgccg gcttcgtcca
     1021 cagaatattt gaggtccacg tttttgagaa gcggcgcata cagatcgtaa taatgcaatt
     1081 ggtcgacgcc gaggatacgc tgacgcagct tcagataacg atggaacgaa ttgagattgg
     1141 cgttgacatt tttgacatgg ttatgataga cgtccgtcgg aatgttattg gcatccagag
     1201 ccgattcgag ggatgaatta tatttacggg catttttgta gaaaatattc tttttgattt
     1261 ccgcatacag ttgtgtgccg taggtgcgac ggaattcatt gagttttccg aagaacgtcg
     1321 aaaaggtttt tttgcggtct tcgcgatttt gtgaagcgcg gaaaaagccg aacgaagcct
     1381 gatcaagctt tactgttttg ccgtcggaaa acgtagcttc gggatatgga aaatcggcat
     1441 ttgaaaaaat gctatagata ttttgtgcac catcggccat caggctggcg tcggctatga
     1501 ttttttcttc cgctgcggaa ccggtatgag cttgacggcg aagaatatcg ttaagataaa
     1561 atttataaat tgccagtttt ggttcctgtt tgataaaacc atccaccatg gcgcgatcaa
     1621 gcgctaagat ttcaggctcg ataaaggaag cggctgcact gaaagaagat ccgatttggc
     1681 tcatctcctg taccattgca agatatttgg aatcgcgcgt atcctgatcc gacgacatgc
     1741 tggcatagga gtacaagcgt gtaaacgttt tggaaagatc aaacatcgta tggagtccgt
     1801 ccaaaagatg ttgggcggat tggccgagcc ggcctttatg cggtgcgagt ttttggatat
     1861 ccaaaatcaa ttgatctttg gactttttcc agtcgtcatc cgatttgtag atgtcggtga
     1921 gattccattt gtatttttct tcgattttgg tgcgatcgcg ctcttgtgca aaagcgaccg
     1981 taccggcaat caagccgatt gtgaaaataa aaagaacttc catcgtatgc atgtaaactc
     2041 cttatgtaaa taaacgtatt ttattaacta aaaaaattcc cgcttgcgcg ggaatgcaat
     2101 cgttcgatga tttatccgat gcgttccacc catccgaaag gttccggctt ttcgccgtat
     2161 tggatggcca gcaattcgtc atatagtttt ttggacaatt cgccgacttt gttttgtccg
     2221 acgatgatgg gttgatcgtt gtgagaaaac tcgccgacgg gtgaaatgac ggctgccgta
     2281 cccgcaccga acgcttccag taggttgccg ttttgtgcgc cttcggccac ttcgtcaatc
     2341 gtgatcggcc gttcggaaat tttcatgccc cagtgtttga gtaattgtat gatcgagtcg
     2401 cgggtgatgc ccggaagaat cgtaccgcct aagggggaag tgacgacttc gtctttgaaa
     2461 cggaagaaaa tattcatcgc gccgacttct tcgatataac ggtgttcaaa agcgtccagc
     2521 cacaatactt gcgtaaaacc ttttttcttg gcttcctgtg cagtataaag acttgctgcg
     2581 tagttggcgc cggttttagc ttcgccgagt ccgcctttgg ccgcgcgaat atgtttttta
     2641 ctgacgtaaa tttttatcgg attaaaacct tccgggtagt agttacctgc cggaccaaga
     2701 agaatataaa acaagaactc atctgaactt ttgacgctga cggacggttc ggtggcgatc
     2761 atggtggggc ggatgtacat cgccgtaccg cgactacggg gaatccaatc ctgatctatc
     2821 aatacgagtt ttttcagcgc gtcgttgaca aattcgatat ctaactccgg catacaaaca
     2881 cgcacggcgg aacggttcat tcgacggaaa ttatccttcg aacggaagag gtacacgccg
     2941 ttgtcttttc cgcgataggc tttaaggccc tcaaaaattt cctgcgcgta gtgaaaaacc
     3001 accgccgccg gatccaaact gagtgcgcga tacggttcga tcatggcttt atgccaaccc
     3061 aacttggttg tgtgggtcat atgaaaaaaa tgatcgctga aaatttttcc gaaaccgagt
     3121 gtggattcgt cggtaggttt gatttttttc ttattttcgt caacgcggat aatgtctaat
     3181 tgcatgtccg ggccctttat aagttaggcg gttaaataaa aagcggcgca atatgtccaa
     3241 gattcgccta ttttgcaata aatatcgtgt aacttaacac tatcggatat gatcaaggtc
     3301 agatttatgc tttttaacgg ttcttaagta accttgaatt cacctacttt tttatatatt
     3361 aatgaagtac ttgattgaaa taggagtttc tgtatctttg tgggtaattt agcggttggt
     3421 atggataaca ataagctgct aaatggagag aaaagtttaa aatttttgta agttttctat
     3481 acgttacagt tttcgattgt agtctttaca gtttttatcg ttgttaaatt ttggaaaaac
     3541 agtgtttagt gctatggcat gctattttca tatgtatctg gcgaacgatt tggattaact
     3601 ttaacaacat tgaaaggaaa gtgtcatggt agccagatcc atcgtagtta aatggaaaac
     3661 aatgaagccg ggaatacttg gcttcgcaag ttttgcttta tgcctcactt ttttggtggt
     3721 gagctgcgaa aaaccggaaa caaacgtcgg aacgaataat ccgggcgatg agcaggtgat
     3781 tacagtatcg tcgaaagtca tttcgaccga ccctaaaatc tctaatagtg gcgatttgcc
     3841 tcattactct caatcgttag gtgtttatta tacaaaatac tataacgtcg gtaatacgcg
     3901 tgaatctatg agtgcgttac ttatccatcg tatcgggaat acatttgtaa gctacggaaa
     3961 ttcgatttct ttgggaggaa aagtgataaa ccgtagtatg gatagttcct caagccaggg
     4021 ttcaacatat tttttctacg gagtagattc ggatattcct gaaggactaa aattggcgac
     4081 gccaccgcaa tttgtggtta gcaattcgga tcaaataaac aacatttcta aaacgttgac
     4141 tacgaaagtg ccgatagcgg cgaccaatat cgcgctgggt acgcatattg atgtatctaa
     4201 tgattttact gttcaattta atcgtgtgtt ggaaaaaggc ggggtttcaa taaatgcgtc
     4261 gggttatatt cgtacctttg aattgaccag ccctgcggat caccttacaa tcccgaaaga
     4321 agaattacaa gccataatcg gcgaaaatgc aacccgaggt gttgtcgtgt ggatatggga
     4381 taattcggtt gtagatacga tcacttcgac tgataaaaac accaacgcag aatattcgct
     4441 cccggtggtt gagtttacag cagtttatgt tgatgacgta catctggata aataatatat
     4501 ctccttcata tcccttacgg gtgggcgtgt tatactaaac gcccactcgt tttttgagaa
     4561 agttgattac ggagccggag ttttgaacaa gttaaaaacc gccattgtct tttttatgtt
     4621 cgtatcggca tgccagttgt ttgctgttga ttcgctgacg tatgttcgga ttacggcgcc
     4681 tgaaattgag tctatgggag gctggcctat ggcaagaaaa tggtatggta cgatgattaa
     4741 tcatttacgt gctcatggtg cacaacagat atttgttgac atatcatttt tgcatgcgga
     4801 tgcacttcac ccggaatccg atgcgttttt ttactccgtc gtcagccgag aatcagacgt
     4861 ctattttctc aatgccgata caagtgctga cacatttagt tttcttggaa tacaccggtt
     4921 accgaaggct cattctttcg gcttattttc tgattttttt gagatttccg ctgatcgtat
     4981 gatttatcac ccttcgcagc gatcattgac ttcgatattg gccggtccgt cagggccgga
     5041 gcgatctgtt gtgatcgaat atcctgatac cgccatagta cctgaaattt cttggctatc
     5101 ggttattcat aacactatag atcggccgct gcatcgtgtt gtggtggcgt tggattatcc
     5161 cggtacgact tcatatttgt acaggccgga tggcgttgcc gtttctacat cttatactca
     5221 gatatgggcg gcgcattgtc tcatccaggg tcactaccga taccgattaa gtacgggcgt
     5281 ttttgtaatg atgttgattc tggtaatcgg tacagttatc gcattaatct catggcgtac
     5341 gtctgtatcg gtttacgtta cgctggtaag tggcggcata ctgatcgcat tagtggggtt
     5401 aatgggttat gcgatcaacg gttggtttta tttgtggatt atgccgggtt tgttaatcgg
     5461 gtcctatcgt ttatggcagc aaataatgcg gacgaatata aaaccgcctg tagcagtagc
     5521 ggtaccggat aaagacgtaa acgaatcaga ttatgcgctc ctcaagtaca aactgaattt
     5581 ctatgaaaat gcagcacaaa ccttgccgga tgatgtattg caaaaatcag acaacgaatc
     5641 cggtgtcttg ttccacgaaa cgtcaccact acgtgaagtt ttagtgaaag cggccggcgt
     5701 ggcatcaagc gatataccgg tgatcgtgca gggcgaatcg ggtaccggta aggaaagatt
     5761 ggcgcaattt attcatgcgc gttcagcccg atcggaattt ccgtttattg ccgttaactg
     5821 cggctcattt aatgaaaacc tcatcgaagc ggagcttttc ggatatgaaa aaggcgcatt
     5881 taccggtgcg catcaaagca aacccggacg atttgagctg gccaacaaag gtacgctgtt
     5941 tttggatgag atcgccgata catcccttgc ttttcaagtc aaactcttac gtgtattgca
     6001 agaaggtgtt ttggagcgtg tcggcgggat aaaacctatt gcagtgtcgg ttcgattgat
     6061 cgtcgctacg catcgggatt tgcgaaaaat ggtacgcgaa ggaggttttc gtgaagattt
     6121 attttaccgt ttacatggat ttgaaatcac gcttccctcg ctgcgtgaac gccgtatgga
     6181 tattgaagta ctggtcgctt cttttttgaa agaagccaat gcgcgcttga cgttctccga
     6241 tgaactgatg tcatggttgt gtcagcaaaa ctggccaggc aatgtgcgtc agcttcagtc
     6301 tgcgacacga cgtgcgatag taaatgcagg attgcgggga cgcacattct tactgccgga
     6361 agattttgag ttaggtacga tcgagaaatt gatgcctaat gaagatacgg ccgaacgtat
     6421 cctgtcggcc tttcgtaaaa atgaatttcg tcatcgtagc gtatccgctg tggcttctga
     6481 tttatccatt caccgtgtta ctgtaacgga gtatctgcgc ggttggattt tgagaggtat
     6541 gagcaaaaaa aacgatgaag aagcaatcat gaatttccta accggcggcg cggtcgttta
     6601 tgatgtcgaa cccttacgac gacgcatcag cgaatacaaa tcctatattg agtcccggat
     6661 tgacgagggg cttcgaaaca atgatagacc ggaaaatttt gtcgccttac gatacaaaaa
     6721 tttacccggt gaatttcatg aagacgccga agctgtatat agagattatc ttacgaatag
     6781 taaattatga gacgcacaaa tatcatatat caattggcga tcttaaatgc actggtgttt
     6841 gcaccagcaa aacacttggc ggcccaatct tcatttttgt ttgggaatgt agattcaata
     6901 tataccaata ttcggtttac cggacattcg acacagatag cggggtcggg tatatactat
     6961 gccaattcgg atataggcat ttcacatcgt cgctcgggtt ggctctggga tgtggttgcg
     7021 cgcatcaatc aggcccaatc ttatcttata gacccgggtc atgtcgtcat ccaactaccc
     7081 gatactgtga cgcttaggga aaacggtgat ctgagatcac gggcccatcg gtatacgtgg
     7141 aataatccgt tttctttgat tgcagggcgc caatggaata agtggtatat cggcacagaa
     7201 caccagcttg tatcgcgcca agaggttctt aaagaaccta ttttacgcga gatcggaaat
     7261 aatggtagaa cctattatct ggaatggaaa acatcgtccc gcacacgtta cgatatttgg
     7321 agtgcggctc atatcggata tcggtggaat aatctgacgg tggacatcgg cacgacattg
     7381 attcctattg tgggtattgg aaaaatgcct aaacaatttg atcaacaaac aaccttgagg
     7441 tggcgcttag gattagcggc acaacattgg ggcggatatt cgaggatttt gtggagtccc
     7501 gatgtgtggt ccgcgagccg tacacagttg tggacgagcc gtataatgac gggtatctct
     7561 atgaccggag agcgcatact ttcacctcgt catattatgc gtgttgatgc cggatatttg
     7621 catcgtgatc gctgggcatt ttatgtcgcc gtgtcatcat cggtagagaa aaatgaatca
     7681 ttgaaacgtg cttcgtttga agacatgttg cgtcacatac atcatggcag cgaaggaagt
     7741 atgacgccac gtgcgatgcg tcctcgtatc tccgcaggca tcgtatatcg tctagataaa
     7801 acaaaacctc cggttatctt gcgtgtaaca cgttctgaat tgctccaaag taatattttt
     7861 gccagtaaaa actcatacta tgcatacaat ccggtagcct ttatcgaatt atataacccg
     7921 gaaacagtac ctgtagacgg ccaaatcatc gcagaaatcg aggatattgg gatacgtttt
     7981 caatcggaag cgctacgttg gcagccgggt gaaatcaaac gcgtgccaat ttttctctac
     8041 tttgatccgg caattttcgg agaagggcat ttggccaaac aactgcgtat cggatttgaa
     8101 aaagacacat atcgttacca gctatcggct tttccggtga cgatacacga tcggcatacc
     8161 tgggacggta atacatgggg actgcgttat ttccttacgc cggaaaaccc cattattcgt
     8221 tcagcggcca agcagttgta tttttccgaa ccagatacgg tcgttacttc tgatttgatc
     8281 aagtttgttc gtctcagaaa tttattagaa aaagcgggga aaaatctacg gtatattgaa
     8341 gatccggtta cgaccattta cgccgatcaa gtacagtatc ccgaagagac acttgtaaaa
     8401 caaagcggtg actgcgaaga catcagtgtt tatatagcct ctctgtgcat gtcggtggga
     8461 tatgcatgtg gtatcgtaga tattcgcccc gatcaaaatg ccggtgtcta tccttcggca
     8521 tccggccgat catcgccggg gcatgtgttc cttttgatag ataccggttt atcgcccgaa
     8581 tgggcggagc gtgccggatt ttccgagttt gatgcgatag cgcgcgataa tacaaaaggt
     8641 gaaacatcgc tttgggttcc gatcgaagcg acgttgctcc atgagggttt cattcgtgca
     8701 catcgcgacg gtgtgcgcca gtactacgat aaagtcattg tacaacaggg cgtaagcaaa
     8761 gggagtgttc atatatatga tttacaataa aatctaccgg ttaatctttt tgacgagtat
     8821 ggtcgcgtgt gcattatatg cgcaggataa agaaagctgg atacaaaaaa catctggtga
     8881 agcactttat ttggaaccgc ggaccggtca atggataccg atttcagcaa aacaaaccat
     8941 accgctgcgc acgtatctga tcacacatca tggatcgaaa acgacattat acgatcaagc
     9001 ggaaatgctg gtgctgcccg atggggcgta tttttttgta gaggacgcca tacctaaaga
     9061 tcgcgatgct ttagtgggag cgcttactca gatcgaggcg gaacatttac caaatcgttt
     9121 ggaatcgcgc cccgtttcgg ttggtcttac ctacggagcg cctgaacata aaaacgaaga
     9181 acagattcca tatctcgctg aacgaattaa tgctgtaaat cggttttatg aattgggtcg
     9241 taaagatgcg tctttgttaa gtctcaaacg actcttgagc aagtatcctc ggttgtatcg
     9301 agataaaaag aacgttgagc aactctttag tctttatgaa aatttggagc tttacggata
     9361 tttgtatgat gagagttcgc gtttgctcaa tctgagtcag gatgccgaat ggtcggtttt
     9421 tatcaatggt tggtacaaaa cggcaaggac gtatgtgact caacagcatt aattcatcta
     9481 tcttaatttt ttcgtagtag tctaaaaact caatcactgg tatccttaga ggcttatata
     9541 tattgtattg atggacaacc aacagtcact catatcggtg aatgtgaatt tagtgagttt
     9601 ctttactact cataccgtaa tgctttcacg ggattgcggg aagccgtacg tacagcctga
     9661 taactgacgg taagtgcggc gatcagtagc gcaacaccgg ccgaggaggc aaaaatccac
     9721 cattccaacg tgatacggaa attgtaatta ttcagccaat cctgcataaa ccatgcggaa
     9781 ggaaggcttg ataccgctgc ggcgatgagt accaagatga caaaatcccg tgcgaaaagt
     9841 agcgcgagtt cggaggtgcg tgcgccgagc gttttacgaa tgcctatttc tttggtgcgc
     9901 tgttcggcca taaaagaagc cagcgcaaac ataccgagac aggcaataaa aatagccagc
     9961 ccggcgaagt atccgataat ccggctcagg ttttcctgag cgcgatacat cttttcgata
    10021 ttctgatcaa gaaatgaaaa ttcgcacgga tattccggaa cgatgcgatt ccacaccgac
    10081 tcaatgtgcg cgatcgcacc ggggagatca tgggcgcgaa tgcgaagcgc catgaacttt
    10141 ctgaagaaat tgaaattacc ggcattgggc atgtctataa aaaaaggctc gatacggtgt
    10201 gcgagtgatt tgtagttaaa atttttgact acaccgatca cgcgttcgtg accgccgatg
    10261 gtttcaaaaa tctgaccgat cgctttttcc ggagaacccc agcccatgcg ttcgaccgcc
    10321 gcttcattga ctaaaatacc cagcgaatca tcgcgcggaa aatcttttga aaaatcccgt
    10381 ccggccagca gttgcaagcc aaatgtttcc gcgaaatctt cgtccaccat gagacctgga
    10441 aaatattgcc attgaccgac aggcataggt tcataacgaa tctcatgggt attatgatgc
    10501 acacccagga tttcattcat tacggtgacg tcttgaattc ggctatcgga tttccattcg
    10561 gacttcagcg tttcataccg tttgatcatc gccggtcgcg taggaataac gaggatgtgt
    10621 tctttttgga atccggtatc ggcggaacgc atataatgta gctggagata gacgacgata
    10681 gtgccggcaa taagaccggt agaaatggca aattgcatca ccacgagagt ctgtctcaac
    10741 caccgcgttt taccggaatg tttgaattgt cctttgagaa ccgtcacggc atcaaatcgt
    10801 gctaaaaaca aggacggata ccatccgccc aaaactccgc acagcgcggc gactaatagt
    10861 ccgaggggta tgtaccatgc atattgaaac gcggcccaac tgaaattttt ccccgtgagc
    10921 gcattcagca gcggtaatga tatttccgac agaataagag cgccaaccca tgcgatcaaa
    10981 ctaatcaaaa gcgattcgcc gagaaactga cgtacgatca tctttcgatc cgcgccaagc
    11041 actttgcgca taccgacttc ccgtgcgcgc atcagcgaac gtgccgtggc gagattggta
    11101 aaattgatac acgagataag caaaatgagc aaaccgacgg tgcagaatac ccatacggtg
    11161 gccgcatccc cgttggggtg catttcataa tctaacttgg aatgcaagtg aatatcggtc
    11221 agaggctgga gatagtgcgt aacctgagct ttgaggaaat tgggataata tttttttata
    11281 aattccggaa attgagcggt caattcatcg ggtgaaacgc cctctttcaa aagcaagtat
    11341 gtccagcacg gattccaaac ccagcctttg aatgtattcg gtccggtcaa ggaagccgcc
    11401 gtagaaaacg aaatcagcgc gtcaaatcga atatggcttt gggtcggtat ttccgcaaaa
    11461 ataccggaca ccatcagatc aagtttgcct tctaatcgaa gtatttttcc caacggcggc
    11521 gtatcaccga aatatttttt ggccatggcg cgggatacga tgatactatt ggggcgggca
    11581 agcgctgttt gcggatcacc ttccgctaag gcgtaatcaa atacttgaaa aaaagtcgaa
    11641 tcggcaaagt acaaattaga ttcgttgtat ttttgatcgc cgatctgcag cgtaaaagaa
    11701 gttaattgga aattaaacag acgtacggtt tgctccacaa gatgcggata gtcagttttc
    11761 aaagcttggg ctaccggaaa cgggttactt gatgaatttt ccgattcgag tttttctgct
    11821 aaacgataaa tgcgatcttt tttgccgtga aagcgatcgt aggaccactc atccaatgcg
    11881 tacaaagcaa taagcagaaa acaagccatg ccgacggcta agccggaaat aagaatagcg
    11941 gaaaaccctt ttttgcgggt aatagaacgc catgcgaccg tcagataatt ggagagcata
    12001 tacgatgacc tcaaactaaa atgatatata aaatagaatg gtgttaaacg aaaggttgat
    12061 ggtaaaaacg ataaaataaa gaatttgttt cggttggagg cgaatatttt atctgctaat
    12121 ttccgcttgt gcatataggt ttttttacct acaatcccgc gtttcggact atttttgcat
    12181 aactcaaaat taatatacga ataaggagta aacgcatggc ttccaaagcc cagacccgtc
    12241 ccgatatggt caggcccggt aacggaaaaa aatattcgct gagcgatttt cccgaattga
    12301 cgaaagaacg gctgttggcc gcgtatcgtc acatgatcat cgcacgatat ttggatgaca
    12361 aaatgttgcg tatgctcaaa caaggaaaga gtttttttca tatcggatgt tccggccacg
    12421 aagccgcgca ggtggcggcg gggtttgcca tgcaacccgg caaggactgg ttttatccgt
    12481 attatcgcga ccaggcgctc tgcctcagcg taggtatgac ggcccaggaa ctcatgttat
    12541 gttttttagc gaaagccgat gatcccaatt ccggaggtcg tcagatgccg caacactacg
    12601 ggcatgcggg tttacgaatt ccttcgcaat ccagtcccac gggaacacag tttttgcagg
    12661 cagtcggttg tgcgatgggc gcggctaaag agggcaaaga cgaagtcgtg tatgtgtctt
    12721 caggggacgg tacgacgtct caaggtgatt ttcacgaagc cctcaattgg gcgagccgtg
    12781 agaaatttcc tgtggtgttc tttattcagg ataataaata tgcgatttcc gtaccgatcc
    12841 atcaacaaac atcggattcg atcctctcgc tcggcgccgg atattcgaat attcattgct
    12901 atgaaatgga cggaacgaat tttctagaaa gctatgccat cataaaagaa gcgacagaga
    12961 aagcacgcaa aggcgaagga ccggctttgg ttgtggcgga tgtcgtccgt ttattaccgc
    13021 actcctcttc cgatgatcag cgtaaatacc gtccggagga agatcttgcg aaagatcgtg
    13081 cgcgcgatcc gatcgaacaa atggccaaat ttctcatcga taaccgcatt cttaaaaaag
    13141 aagacgatga agcgtttcgt caggaatgtt ataaatctgt agatgacgca gcggattggg
    13201 ccgaaacgca gccgcacccg gataaaagcg ttgccatgaa acatgtgttt tctgaagaac
    13261 cggatacgct cgcatatgaa aaaaccgtac cgagcggaaa acccatcgtg cttgtggatg
    13321 cgatcaatca tgctatggcc gaagaactgg agcgcgatcc cagaattatt atgttcggtg
    13381 aagatatcga agatgacaaa ggcggcgtat ttacggcaac gaaaggcctg tccaaaaaat
    13441 ttggacgcga tcgcgtgttc aattcaccgc tagcggaatc atccatcgtc gggacggccg
    13501 tagggctcgc cgtgcgcgga tggaaacctg tggtagagat tcagtttggc gattatgtgt
    13561 ggacggcgat gatgcagatt cgcaacgaac tcgcgacgat gcgttaccgt tccaacggta
    13621 cgtggaaatg tccgatggtc gtgcgtattc ctataggcgg ttatattcac ggtgcgcttt
    13681 gccattcgca gaatatcgaa tccttttttg cacatattcc cggtttgaaa atcgcattac
    13741 cgtcgaatgc agccgatgcg aaagggcttc tgaaaacggc cattcgttgt gatgatccgg
    13801 tgttgttctt ggaacacaaa ggcatgtatc gtcaaggttt tgcacagatg ccggaaccgg
    13861 atgcagatta tttgcttcct ttcggcaaag cggccgtcaa gcgtgagggt tcggatatca
    13921 cggtcgtgac gtacggtatg atggtcaata aatgcctcaa tgctgcgaag tacatggaag
    13981 aaactcacaa tgtgaaaatc gaagtgattg acattcgtac gatcgtgccg ttggataccg
    14041 atacgatttt gaattccgtc aagaaaacag gcaaatgcct tgtggtgcac gaagatgtcg
    14101 aattttgtgg ttttggcagc gaaatcgttg cgcagatcat gcaccaagga tttgaatacc
    14161 tcgatgctcc ggtccaacgt gtggctggcg ctttttcacc ggtgccgtat aactggttcc
    14221 tcgaagaaat gatacttccg caggatcata acattacgac cgcgcttgaa aatttagcaa
    14281 agtactagga ttgatttgta actcccgccg tatgttctcc atgcggcggg atgatttttt
    14341 attaaacctt acggccttat ctctgtctaa tacagataca ttcctctttt acttaacata
    14401 ggtagtcaat gatttgtttg ctataacggt ttcgtagttt ttaggtcaat ctttcatttt
    14461 tcacaaatta gagggaattc tcatgagaaa agtcattttt ctttgcttag ttgcactatt
    14521 aatgcaggat caattatccg cacagacaag tgtaggcttt tcagataata atcccaaacc
    14581 gatcaaggat taccggttgc ccgattgggg atatagccgc ggatggatca atgcgagcct
    14641 gagtagaaac aatagctctt cggattattc tgatgaagaa tataagaatg ataactcgtc
    14701 gtattggcta aattccaatt atatccttat gcgtgaatcg gaaaagcagg atttaaactt
    14761 ttcagcttcg gcattttatt ccaaacgaac caatgaaacc agtagaaata acgatctagg
    14821 tcactctgac cgtgactata atgacatagt tgctcatgtt atgttgcgag gaaatagttc
    14881 ccactatgcg ggatattatc atgccaccat tctaccgcac atagcttata caaagagcga
    14941 gtctgtttat aaagtaacgg atatcggtta tatgtttcct gatacggcca taatgcgttc
    15001 taaagaaaaa tcaaacgcca ttgatttgtc tttggcgaca ttgtttggta ttggccgaat
    15061 ccgtaacgtg acacctgtca ttcgcgccat gagagtgcat gaaaggctca acgctgtcgg
    15121 aaagggtgaa tcatggaacg aagaaacaac gcagcgtgtt gccgcagagt ttgcacggtt
    15181 atatggttat caaaatgtct atgataggca tcaaaaagaa ttctggaact cgttagatga
    15241 agcattaccg gat
//
LOCUS       DAOQGF010000082        15027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1828_length_15027_cov_6.565522, whole genome shotgun sequence.
ACCESSION   DAOQGF010000082 DAOQGF010000000
VERSION     DAOQGF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_15027_cov_6.565522"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PK937_11200"
     CDS             <1..996
                     /locus_tag="PK937_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNH33182.1"
                     /translation="HDEMLFIIIHQAYELWFKQILHEVDSVARLFAEDHIDEKNIGIA
                     VARLQRVTEIQKILVDQLRILETMTPLDFLEFRDFLVPASGFQSVQFRLIENKLGLKH
                     SQRLNYGQHPYHDRLSKEHQSLVQTAEQSASLLELVERWLERTPFLELGDFNFWSAYR
                     GAVTRMLDEDHRTIEHNVSLSPSKKEEQLKQLDLTRINFDALFDENKHNQLMKEGVRT
                     ISYRATQAALLICLYRDQPILHQPFRLITLLVDIDEMFTTWRYRHALMVHRMIGTKIG
                     TGGSSGHQYLKGTSDQHKIFSDFFNLSTFLIPRSSLPKLPGDVERNLGFVYSQKQ"
     gene            1021..2334
                     /locus_tag="PK937_11205"
     CDS             1021..2334
                     /locus_tag="PK937_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH33183.1"
                     /translation="MSAIVDMIRKAGVVGAGGAGFPAHIKAGSTVDTVIANGAECEPL
                     IHKDYELMLHYPAEVVRGVELVMQSTGAKEGILGVKEKNTKAIEALQKHLKPGIRLHL
                     LGDFYPSGDEYILVYESTKRLIPPQGIPLHVGCVVNNVETFYNIARAEKGEAVTEKFI
                     SVSGAVKKPFTGIVPVGATFADVIAMAGGITEEEYGVFVSGIMMGKLTFDLDQPITKT
                     CAGLIVLPKEHKLVTRKSLPEESMHRIGKSACDQCSYCTELCPRYILGYDIQPHKVMR
                     SLGFTKTGTDYWNQFASLCCACGLCTLYACPESLFPKEACDQSKRVMKEQNIKWSGPV
                     EIQPHPMYEGRRTPLKMLVKRLGVTDYDIHAPYSDRQATPFKRIILPLQQHVGQKAEA
                     VIRTGESVKRGQLIADVPQDKMGAPIHASIDGTARVEADHILIES"
     gene            2358..2909
                     /locus_tag="PK937_11210"
     CDS             2358..2909
                     /locus_tag="PK937_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNH33184.1"
                     /translation="MTMNSIGLIELGSIAAGFDTADAMLKAANVNILLARSICSGKYI
                     VMVGGEVAAVQSSIDAGAAQSRGSVIDTFVIPNVHPEVFPALSGAGRSELRESLGIIE
                     SFSVASLIEAADAAVKAANVKLLEIRLAMALGGKAFLTMTGEVAAVQAAVDAGSRVVA
                     EKGLLVNKVVIPGPRPELLTEVV"
     gene            3009..3797
                     /locus_tag="PK937_11215"
     CDS             3009..3797
                     /locus_tag="PK937_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH33185.1"
                     /translation="MNLNLQNRTALVCGSTQGIGKAAAMALAGLGARIILCARNAEAL
                     ENVKNELPQPASAHHTYLVADFSKPETLQQILHTFTEKSGPIHILVNNTGGPAGGPIA
                     DAATDAFEQTFRQHLICNHILAQAVIPGMKSSGFGRIINVISTSVKQPLKGLGVSNTI
                     RAAVANWAKTLSVELAPFGITVNNVLPGATLTQRLTTLIANRAKQSGRPEEEIQKEMV
                     AEIPAGRLGQAEDIAAAIAFLASPAAGYINGINLPVDGGRTGCL"
     gene            3801..4385
                     /locus_tag="PK937_11220"
     CDS             3801..4385
                     /locus_tag="PK937_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HNH33186.1"
                     /translation="MISAPEPSELLRKVFAAHTENYGDPDVQFHFDQQNSTLTALNYL
                     DVFVWEPTEEIPMTTFSTIGMAERAMPECPHRAEIHWTLRGKLTEAQEEEAAVFLLNL
                     AALPFVKNTFLDHWHVLPNLRIPHFSGCAAGLMHPSFVDGGWDHMHAENTIVKFLNFV
                     PLTQSELDMARTMGVRRMLNALYESQTDIFSDRM"
     gene            4398..5282
                     /locus_tag="PK937_11225"
     CDS             4398..5282
                     /locus_tag="PK937_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNH33187.1"
                     /translation="MEELLQIVSDAYAGYSNYLYMEMTGASVPWYRNYFYGLIAISLV
                     VWAFEIIIPWRKNQPVFRRDFWLDGFYMFFNFFLFSLIAYNALSNVCVVWLDRGLETI
                     GIGSLSFIKVNTWPVWLQYILMFVVADFIQWNIHRLLHRVPWLWEFHKLHHSVKEMGF
                     AAHLRFHWMETVIYKSLQYIPLAFFGFGLTDFFMLHMIALAIGHLNHANIAWTWGPLR
                     WLLNSPVMHIWHHAKHLPGSYPYGVNFGLSLSVWDYLFKTAYIPHDGRDIELGFENEE
                     PFPRGFFKQVIYPIGKQN"
     gene            5385..6827
                     /locus_tag="PK937_11230"
     CDS             5385..6827
                     /locus_tag="PK937_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNH33188.1"
                     /translation="MKTISNYIDGKLQPALSGKTLDNYEPAVGAVYGTIPDSDERDVA
                     MAVDAAEKAFPAWSLMPAEQRARLLFKLSELIDRDMEKLVRAESIDNGKPLTLARAMD
                     IPRASSNFYYFASAILQFSSEAHAMENTAINYTLRQPIGVVGCISPWNLPLYLFTWKI
                     APALAAGNCVVAKPSEITPMTAFMLSELAIEAGFPKGVLNIVHGLGPKVGAAITTHPA
                     ITAISFTGGTKTGAEIARVAAPVFKKLSLELGGKNPNIIFDDCDYDEMLTQTMRSSFA
                     NQGQICLCGSRLLVQRGIYDRFLKDFSARTKKLVIGDPLDESTQQGAIVSQAHMEKIL
                     YHIDLAQKEGGRIVTGGHPVKLSGRCTNGWFIEPTIIEGLGAQCRTNQEEIFGPVVTV
                     MPFDTEEEALALANSTSYGLASTVWTRDLQRAHRIGGAIKSGIVWVNCWLLRDLRTPF
                     GGVKNSGVGREGGFEALRFFTETKNVCVKI"
     gene            6883..7227
                     /locus_tag="PK937_11235"
                     /pseudo
     CDS             6883..7227
                     /locus_tag="PK937_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            7291..7698
                     /locus_tag="PK937_11240"
     CDS             7291..7698
                     /locus_tag="PK937_11240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNH33189.1"
                     /translation="MLNSSKAPEPVGLYPHARRVGNLLFLSGVGPRERGKKTIPGVTL
                     DAKGNIKKYDIAKQCHSVFRNIRYILEDAGSSWDNIVDVTVFLTNMKEDFPIYNKIYA
                     EYFKNNKPCRTTLEINALPTPIAIELKVIATID"
     gene            7771..9306
                     /locus_tag="PK937_11245"
     CDS             7771..9306
                     /locus_tag="PK937_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH33190.1"
                     /translation="MKKFISPLRILWFCVLAPMLPAQTKLDKDSLSRYANAALAQWNV
                     PGMAISVVLNDSMIYAKGFGVKTFGKNDPVDANTNFSIASLTKAMTAASMGILVDEGR
                     VRWDDPMSNYLPEFKMYDPYVTQAMTIRDLLSHRSGLETFSGDLVWYGTHYDRSEVLR
                     RARYLQPKYGFRAKYGYQNIMYLAAGEVVGRVSGKGWDDFIRERIFIPLGMTRTITRF
                     ADIRTMTNIATPHAIVDFKPVPVEYRNWDNVAPMGSTVSNVVDMAQWIRLQLGRGTYE
                     GRTIYSTAVAREMWQPHTLMDISENGERAMPSRHFLAYGLGWTMHDYHGKKILTHSGG
                     ADGMVSRLVLIPEDRFGFVILTNSINGLTTPMIYHIIDMHLGKNEKDWDAFYWEFNKR
                     AELKEIADRKKTDSLRVKNTKPTLKIEAYAGIYGGELYGDVEVKTEKDKLTIRFLPTS
                     TFTGTLTHWHYDTFQITLQDKNLPTGFATFTLDAAGKPDELKIDIPNPDFDFTELKLK
                     RKR"
     gene            9688..10218
                     /locus_tag="PK937_11250"
     CDS             9688..10218
                     /locus_tag="PK937_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33191.1"
                     /translation="MESPILTETPRLLIRPMHASDAEMFFDLFGSAETLIHFPRAYTR
                     DEVHRLIRRQQERYTSRGYGLWTLVARDSGEIIGDCGLIPQRVDACEEVELAYHLRKK
                     FWHQGYATEAGAAAIAWVEAHNHAASLIALIRPENVPSRHVAERLGFIKDTAIFHAGL
                     LHDVFRRAIVPQPVSV"
     gene            10271..11662
                     /gene="cysS"
                     /locus_tag="PK937_11255"
     CDS             10271..11662
                     /gene="cysS"
                     /locus_tag="PK937_11255"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNH33192.1"
                     /translation="MPSLQLFDTYTRALRNFEPLQPGEVGMYTCGPTVYDYAHIGNLR
                     TYIFEDILRRVLEYNGYKVHHVMNITDVGHLTSDADTGEDRMEKGSRRTGKTAWEIAA
                     EYTEEFKKDMKRLNITEPHTWCKATDHIPEQIEFIRGIENKGYTYRTSDGIYFDTSKL
                     DDYGYIARLKVEGLEAGSRVEIGEKRNITDFALWKFSKPEEQRQMEWDSPWGKGFPGW
                     HIECSAMSAKYLGSYFDIHCGGEDHIMVHHPNEIAQTQACYGTRLANFWMHGYFLQID
                     NSRMGKSVGNFLRVQTLIDQGYDPIVWRMFCLSAHYRTKLNFNWESLDGSATALQRLR
                     TAVYEWGAAGTPDEATLTQFRTFINDDLNIPRVLALVWDLVKSDKTSATKKATVLEMD
                     RVLGLGLAAWVPAVEEIPAEIQAWADARVLARKEKRWADADTFRDKITAAGYDIKDTP
                     QGIKVEKRKTPGA"
     gene            11662..12705
                     /gene="dusB"
                     /locus_tag="PK937_11260"
     CDS             11662..12705
                     /gene="dusB"
                     /locus_tag="PK937_11260"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNH33193.1"
                     /translation="MNIDNVNIHDFPVSLAPMEDVTDIAFRIVCKRLGADILYTEFTS
                     SEALIRDARKALRKIQVADEERPVAIQLFGGLDTSMEGAARIAEKANPDFIDINCGCW
                     VKDVAMRGAGAGLLKDLPRFEAIVRSTVRGTQLPVTVKTRLGWDKNTINILDVARMVE
                     DAGAKALTVHCRTRDMGHDGSADWHWLEKIKKVISIPLFGNGDVKTPQDAKRMLETGC
                     DGVMIGRAAIVNPWIFRESRHYIDTGEILPPPDAEERLRVCIEHLRLSVKYKEEKYGV
                     LEFRKYYSGYLKGLPYIGKLRAELMQYTTLQPILDRLAQYGEEIKQWQASEGRDAMLP
                     QLGGLIGTRHETV"
     gene            12944..13276
                     /locus_tag="PK937_11265"
     CDS             12944..13276
                     /locus_tag="PK937_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNH33194.1"
                     /translation="MVITFKNKKISALVNNDRKLLKEMGKKRADKIRQRLDQLQAAET
                     LEDLRHVAGNYHELTGDRKGQWACDLDQPYRLIFIPHENPVPVNTSGQYIWLEIRGVE
                     IIEITDYH"
     gene            13288..14403
                     /locus_tag="PK937_11270"
     CDS             13288..14403
                     /locus_tag="PK937_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNH33195.1"
                     /translation="MIRANEYFPQSVTHAGADLDEKLKELGMGVKEFAIRTDKPEKTI
                     IAVIKGDSAITPDMAVQFEQVLKIPAHFWLNRQRQYDESIARSKQKEMIQEAEDWASS
                     FPLKEMLKLKWIRSFSSKSEAVTALFEFFGISSPAAWESLYLRSELKVQFRISLAHTK
                     EHHALSAWLRRGERQAGELDVPDYSEKNFKQALPEIKTVMAKHPKDFFTKLQSLCKEA
                     GVKVVHTPCLPKTPISGATRWLNDTPLIQMSGRYKRNDSFWFTFFHEAGHILLHGKKD
                     IFLENVQYGDRDDEKEEEANFFSQKWVFSESEKTNVLSYADKTGRLTHADVIEFAKEF
                     NTHPAMIIGRLQHDQKIPYSLGREFIKPVELDKNSDC"
     gene            complement(14512..>15027)
                     /locus_tag="PK937_11275"
     CDS             complement(14512..>15027)
                     /locus_tag="PK937_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH33196.1"
                     /translation="RALDFIWVALAALGIALLNPFSGHVDPVGFVLALVAGACWGLYI
                     ILGEKLGSATSGSVGLTIAMCVGSLVLIPVKIADAPQWLNIVPVLPFALVVAFLSSVI
                     PFSFEIEALRRMPPKVFGILMSMEPAYAAVIGFLLLDEYLTLSQCIAIGAVVAASVGA
                     ARTGSSVPTDV"
BASE COUNT         4006 a         3725 c         3638 g         3658 t
ORIGIN      
        1 cacgatgaga tgctgtttat catcattcat caggcgtacg aactttggtt caaacaaatt
       61 ttgcacgaag tagattccgt tgcgcgtttg tttgcagaag atcatattga tgaaaaaaac
      121 atcggcatcg ccgtcgccag attgcagcgc gtcacggaaa tccagaaaat cctggtagat
      181 caactgcgga ttttggaaac gatgacaccg ttggattttt tggaatttcg tgatttttta
      241 gtcccggctt cgggttttca aagcgtccag tttcgcctga tcgaaaacaa actcggtctc
      301 aaacattccc agcgactcaa ttacggtcaa catccatacc acgatcggct ctccaaagaa
      361 catcaatctt tagtgcagac tgctgaacaa tcggcttcgc tgctcgaatt ggtcgagcgc
      421 tggttggaac gcacaccgtt tttggaattg ggtgatttta atttttggag cgcataccgg
      481 ggtgccgtaa cgcgcatgct ggatgaagat caccgcacca tcgaacataa cgtaagtctt
      541 tcgccatcca aaaaagaaga acaattaaaa caacttgatc tcacgcggat caattttgat
      601 gcactcttcg atgaaaacaa acataaccaa ctgatgaagg aaggcgtacg cacgatttcg
      661 taccgcgcca cgcaggcagc gctactgatt tgtttatacc gcgatcaacc gatattgcat
      721 cagccgtttc gcctcattac tttacttgtg gatattgatg aaatgtttac aacgtggcgt
      781 tatcgccatg cactgatggt acaccgcatg atcggcacca aaatcggcac gggcggttcg
      841 tccgggcatc agtacctgaa aggcacttcg gatcaacata aaatcttttc cgattttttc
      901 aatctttcga catttttaat tccgcgttcc tcattaccca agctgcccgg cgacgtcgag
      961 cgcaatcttg gatttgtcta cagtcaaaaa caataaggat cgttaaccga taaaacacct
     1021 atgagcgcta ttgttgatat gatacgcaaa gccggtgtgg tcggcgccgg cggagccggt
     1081 tttccggcgc acatcaaagc cggatccacc gtggataccg taatcgccaa cggcgccgaa
     1141 tgcgagccgc tgattcacaa agattacgaa ttgatgttgc attatccggc ggaagtcgtg
     1201 cgtggcgtcg aactcgtcat gcaatccacg ggcgccaaag aaggcatcct cggcgtcaaa
     1261 gaaaaaaaca ccaaagccat cgaagcacta cagaaacatc tcaaacccgg catacggctt
     1321 catctgctgg gtgattttta tccttcgggc gacgaatata tcctcgttta tgaaagcacc
     1381 aaacgcctga tacctccgca aggcataccg cttcatgtcg gctgcgtggt caacaatgtc
     1441 gaaacgtttt acaacatcgc gcgggccgaa aaaggcgaag ccgtgacgga aaaattcatc
     1501 tccgtttccg gcgcggtaaa aaaaccgttc accgggatcg ttcccgtcgg tgcgacattt
     1561 gccgatgtga tcgccatggc cggcggcatt acagaagaag aatacggtgt ttttgtctcc
     1621 gggatcatga tgggcaaact gacctttgat ctggatcaac ccatcaccaa aacctgcgcc
     1681 ggattgatcg tacttcccaa agagcataaa ctcgtgacgc gaaaatctct accggaagaa
     1741 tccatgcatc gtatcggcaa atcggcttgc gatcaatgca gctattgtac tgaattgtgt
     1801 ccccgttata ttttgggtta tgatattcag ccgcacaaag tgatgcgcag cctcggattt
     1861 accaaaaccg gtacggatta ttggaatcaa tttgcctctt tgtgttgtgc ctgcggctta
     1921 tgtacactgt atgcatgtcc ggaaagtctc ttccccaaag aagcctgcga tcaaagcaaa
     1981 cgtgtgatga aagaacaaaa tatcaagtgg tcaggcccgg tggaaataca accgcatccg
     2041 atgtacgaag ggcgccgcac cccgctcaaa atgctggtga aacgtcttgg cgtcacggat
     2101 tacgacatac acgcccctta ttccgatcgt caagcaactc catttaaacg tataatcctg
     2161 ccgctgcagc agcacgtcgg acaaaaagcc gaagcggtta ttcgcacggg tgaatcggta
     2221 aaacgggggc agcttatcgc cgatgtcccg caggataaaa tgggcgcacc gatccacgcc
     2281 agcattgacg gtacggcacg cgtcgaagcg gaccacatcc ttatcgaatc ttaaaatgta
     2341 aatttgagga tattatcatg actatgaatt ctatcggatt gatcgaactc ggcagtattg
     2401 cagcgggatt tgacacggcg gatgcgatgc tcaaagcggc taacgttaac atcttactgg
     2461 cacgttcgat ttgttcaggc aaatacatcg tgatggtggg cggtgaagtc gcggcggtgc
     2521 aatccagcat tgatgccggt gcagcacaaa gccgcggttc ggtcatagac acctttgtca
     2581 ttcccaatgt gcatccggaa gtttttcctg cgctctccgg agcgggccga tccgaactgc
     2641 gcgaatcgct gggtattatc gaatcgtttt ctgtggcttc tttgatcgaa gccgccgatg
     2701 ccgctgtcaa agccgccaat gtcaaattgc tggaaattcg cctcgctatg gcgctcggcg
     2761 gtaaagcgtt tctcaccatg accggtgaag tcgccgctgt tcaggccgct gtagatgcgg
     2821 gttcacgtgt cgtggccgaa aaaggactac tcgtcaacaa agtcgtcata ccgggtccgc
     2881 gaccggaact gctgaccgaa gtagtctgat cagataattt tctctcgcgg agacgcaaag
     2941 tacacgacgt cgttccagtt tgtatctttt tttcatcgcg tctttgcgag caaatactat
     3001 aaacagacat gaatctcaat ctgcaaaata gaaccgcctt ggtgtgcggc agcacgcaag
     3061 gcatcggtaa agccgccgca atggcgctgg ccggtctcgg cgcgcgaatc atactttgtg
     3121 cacgtaacgc cgaggcgttg gaaaatgtaa aaaacgaact gccccaaccg gcttcggcgc
     3181 accatacgta cctggtggca gatttttcaa aaccggagac gcttcagcaa atcctccata
     3241 cgtttaccga aaaatcaggt ccgatacaca ttctcgtcaa taataccggc ggtcccgcgg
     3301 gcggcccgat cgcggatgca gcgaccgatg catttgagca aacctttcgc cagcatttga
     3361 tttgcaacca catcctggca caagcggtaa tccccggtat gaaatcatcg ggttttggtc
     3421 gtattatcaa tgtgatctcg acctccgtca aacagccgct caaaggttta ggcgtatcca
     3481 ataccattcg cgccgcagtg gccaactggg caaagacatt atcggtcgaa cttgcaccgt
     3541 ttggcataac ggtgaacaac gttttgcccg gcgccaccct cacacaacgc ttgaccactc
     3601 tcatcgccaa ccgcgccaaa caaagcggtc gcccggaaga ggaaattcaa aaagaaatgg
     3661 tggccgaaat tcccgccgga cgcctggggc aagcggaaga tattgctgcc gccatcgctt
     3721 tcctcgcctc acccgcggcc ggatatatca atggtatcaa tctgcccgtg gatggcggaa
     3781 gaacgggatg cttataaatc atgatctccg cgcctgaacc ctccgagctg ctacgtaaag
     3841 ttttcgctgc gcatacggaa aattacggtg accccgatgt acagtttcac ttcgatcagc
     3901 aaaattcaac gctgaccgcg ctcaactacc tcgacgtgtt tgtttgggaa ccgacggaag
     3961 aaatccccat gacgacgttt tccaccatag gtatggccga acgtgcaatg ccggaatgtc
     4021 cgcatcgcgc cgaaattcac tggacgctgc gcggcaaact gacggaagca caagaggaag
     4081 aagccgctgt ttttttactg aatctcgccg cgctgccttt tgttaaaaat acttttttag
     4141 atcattggca tgtattaccc aacctcagaa ttccccattt tagcggttgc gcggccggat
     4201 tgatgcaccc ttcgtttgtg gatggtggct gggatcacat gcacgccgaa aataccatcg
     4261 taaaatttct taattttgtc ccgctgacgc aaagcgaatt agacatggcg cgtacgatgg
     4321 gagttcgtcg tatgcttaat gcgttgtatg aaagtcagac ggatattttt tccgatcgca
     4381 tgtgacaggg agcgcttatg gaagaactgc ttcagatcgt ttcagacgcc tacgcgggtt
     4441 attccaatta tctttacatg gaaatgacag gggcctccgt cccatggtat cgtaattatt
     4501 tctacggtct gatcgcaatt tcccttgtcg tatgggcatt tgaaatcatc atcccgtggc
     4561 gtaaaaatca gcccgtgttt cgccgcgatt tttggctcga tggcttttat atgtttttta
     4621 attttttttt gttttcactg atcgcgtaca acgctttatc caatgtgtgc gtcgtatggc
     4681 tggatcgcgg attggaaact atcggtatcg gcagtctgtc ctttatcaaa gtaaacacat
     4741 ggccggtatg gctacagtac atacttatgt ttgtcgtggc agattttatt cagtggaata
     4801 tccaccgctt actgcaccgc gttccgtggc tgtgggaatt tcacaaactg catcactcgg
     4861 ttaaagaaat gggatttgcg gcacatttgc ggtttcactg gatggaaacg gtgatttaca
     4921 aatcgcttca gtatataccc ctcgcgtttt ttggttttgg gttgacggat ttttttatgc
     4981 tgcatatgat cgcacttgcg atcgggcatc tcaatcatgc caatattgca tggacgtggg
     5041 gtccgctcag gtggctgctc aatagcccgg ttatgcacat ctggcatcac gccaaacatc
     5101 ttccgggatc gtatccgtat ggcgtcaatt tcggattatc actgagcgta tgggactatc
     5161 tttttaagac ggcatacata ccgcatgacg ggcgcgatat cgaacttggt tttgaaaatg
     5221 aagaaccttt cccgcgcggc ttctttaaac aagtaatata tcccatcggc aaacaaaatt
     5281 gaaatatgta cgcatcacac atctatacct taaagaagtg attttactaa gcgaccgtat
     5341 ccgatggatt ttatttttta ctatgctaac caaccgtgat ttttatgaaa actatttcga
     5401 attacattga cggaaagctc caacccgcac taagcggaaa aactttggat aactatgaac
     5461 cggcggtggg cgccgtctat ggcacgatac cggacagcga tgaacgcgat gtcgcgatgg
     5521 ccgttgatgc ggctgaaaaa gcttttcctg cgtggtcttt gatgcccgcc gaacagcgtg
     5581 cgcggttact tttcaaactt tcggaactga ttgatcggga tatggaaaaa cttgtccgcg
     5641 ccgaatccat agacaacggt aagccgctga cccttgcgcg ggcgatggat ataccgcggg
     5701 cttcgagtaa cttttattat tttgcttcgg cgattcttca gttttccagc gaagcgcatg
     5761 cgatggaaaa tacggcgatc aattatactt tgcgtcagcc gatcggtgtc gtgggttgca
     5821 tttcaccgtg gaatctgccg ttgtatcttt ttacctggaa aatcgccccg gcgctcgcgg
     5881 cgggaaattg tgtggtggcc aaaccgtcgg agataacacc gatgaccgcg tttatgctgt
     5941 ccgaattagc catcgaagcg gggtttccga aaggcgtact gaatatcgtt cacgggctcg
     6001 gtccgaaagt aggcgcagcg atcacgacac accccgcgat cacggcgatt tcatttaccg
     6061 gtggcacgaa aaccggcgcc gagattgccc gtgtggccgc accggtattt aaaaaacttt
     6121 cgctcgaact gggcggtaaa aaccctaaca tcatatttga tgactgcgat tacgatgaaa
     6181 tgctgacgca aaccatgcgc tcttcttttg ccaatcaggg gcaaatctgt ttatgcggtt
     6241 cgcgtctatt ggtccaacgc gggatatacg atcgttttct caaagatttt tccgcgcgca
     6301 ccaaaaaact cgtgatcggc gacccgctgg acgaatccac gcaacaaggg gcgatcgtat
     6361 cgcaagcgca catggaaaaa atattatatc acattgacct tgcacaaaaa gaaggcggac
     6421 gcatcgtgac aggcggtcat cccgtaaaat tatccggccg ctgtaccaac ggttggttca
     6481 ttgaaccgac aatcatcgaa ggtttgggcg cccaatgccg caccaatcag gaagaaattt
     6541 tcggccccgt cgtgaccgtg atgccgtttg acactgaaga agaagcgtta gcactggcca
     6601 atagtacttc gtacggactg gcctctaccg tatggacccg cgacctgcag cgtgcgcatc
     6661 gtatcggcgg cgcgatcaag tccggcatcg tatgggtcaa ctgctggctg ttgcgcgact
     6721 tgcgtacgcc tttcggcggc gtcaaaaatt ccggtgtcgg acgcgaaggc ggcttcgaag
     6781 ccttgcgctt ttttacggaa acgaagaatg tgtgtgtgaa gatttagaat ttagaatttc
     6841 ggatcgttga ttttcgaacg tttacaaaat acacatagtt ttatgaattc gatggacttt
     6901 aagttaagaa caaaagattt tgcaaaacgt gttatcgccg tatgccgggc attaccgccc
     6961 aatcgggagt cgcagttgat acaacgccaa ttgttccgat cgggaacatc cgtcggagcg
     7021 aattaccgtg aggcctgcag agcgcgatct acggctgaat ttatagcgaa attaggtatc
     7081 gtagaagccg aagccgacga aacactgtac tggctggaac ttattgcaga actggaatta
     7141 cttcctaaaa ttaaactcaa acagctaatt atcgaaaatc acgagatcat cgctatgaca
     7201 gttgcgtcaa ttaaaacatt aaaacaaaac aaaacgggag ataaaaaaac cgacactccc
     7261 caatcccaaa tcccaaatct taaatcggga atcctgaatt cctccaaagc gccggaaccg
     7321 gtcggcctgt atccgcatgc acggcgcgtg ggcaatctat tattcctgtc gggcgtcgga
     7381 ccgcgagaac gcggaaaaaa aaccatcccc ggcgttacac tggatgccaa aggtaatatc
     7441 aaaaaatacg atatcgccaa acaatgtcat tccgtgtttc gcaatatacg ctatatcctt
     7501 gaagatgccg gttcatcctg ggataatatc gtggacgtca cggtatttct gaccaacatg
     7561 aaagaagatt tcccgattta taataaaatt tacgcggaat attttaaaaa caataaaccc
     7621 tgccgcacga cgctggagat caacgcgctg cctacaccca tcgcgatcga acttaaagtc
     7681 atcgcaacaa ttgattagtt gagatggtaa ctttaaaggc taccgtgcgt aaaacgatac
     7741 attaaaactc tattagtgag agagcactgc atgaaaaaat tcatttcgcc cttgcgcatt
     7801 ctttggtttt gcgtgttagc accgatgctt ccggcccaga ccaaactgga taaggatagt
     7861 ctttcccgct atgcaaacgc cgcgctcgcg cagtggaatg tacccgggat ggccatttcc
     7921 gtcgtactca acgactcgat gatttacgcc aaaggcttcg gtgtaaaaac cttcggaaaa
     7981 aatgatcccg tagatgccaa tactaatttt tcgatagcct cgctaaccaa agccatgacg
     8041 gcggcgagca tgggcattct ggtggatgag ggtcgcgtgc gttgggatga cccgatgtcc
     8101 aactatcttc cggaatttaa aatgtacgac ccttacgtca cgcaggccat gaccatccgt
     8161 gatttattga gccatcgtag cggtttggaa acatttagcg gcgatctggt gtggtatggt
     8221 actcattatg accgttcgga agtgctgcgc cgggcaagat atttgcaacc caaatacggc
     8281 ttccgtgcca aatacggtta tcaaaatatc atgtatctcg ccgcaggaga agtcgtagga
     8341 cgcgtatccg gtaaaggttg ggatgatttt attcgtgaac gtatttttat ccctttgggt
     8401 atgacccgga cgattacacg ttttgccgat atccgcacga tgaccaatat tgctacaccg
     8461 catgcgatcg ttgatttcaa acccgtgcct gtcgaatatc gtaattggga taatgtcgca
     8521 ccgatgggct ccaccgtttc caatgtggta gatatggcac agtggattcg cctccagttg
     8581 ggacgcggca cttacgaagg gcggacaatt tatagcaccg ccgtcgcgcg tgaaatgtgg
     8641 caaccgcaca ccctgatgga tatttcagaa aatggagaac gcgctatgcc gtcccggcat
     8701 tttttagctt atggcttggg ctggactatg cacgactacc acggtaaaaa aatactaaca
     8761 cactccggtg gcgccgacgg tatggtctca cggctggttc ttatccccga agaccgtttc
     8821 ggttttgtga tactcaccaa tagtattaac ggcctcacca caccgatgat ctatcacatc
     8881 atagatatgc acttgggtaa aaacgaaaaa gactgggatg ctttttattg ggaattcaat
     8941 aaacgcgccg aactgaaaga aattgccgat agaaaaaaga ccgacagtct gcgcgtgaaa
     9001 aacaccaaac cgacgctcaa aatcgaagcg tatgccggta tttacggcgg tgaactctac
     9061 ggtgacgtcg aagtcaaaac agaaaaagac aaactgacga ttcgattttt gccgacgtcc
     9121 actttcaccg gtacgctcac gcattggcac tatgacacgt ttcagatcac gcttcaggat
     9181 aaaaatctac cgaccggatt tgccaccttt acgctggatg ccgccggcaa accggatgaa
     9241 ctgaaaattg acatccccaa cccggatttt gattttacgg aattgaaatt gaaacgaaaa
     9301 agataagata atgtgtgttt ttacgcacca acatcaaatg catgtagagt ttccaaaccg
     9361 gcacggagta ttaaatttgg atagggtcaa ttaggcctta agaaaaaggc cgctcggaac
     9421 tctcggttgc gcattatcat cggcgaaaaa cctgtttgac ctcgccatcg gcgggggagt
     9481 tgtttttcgc caccagtaag caaccgaggg tttaggcgtt tttctcacag ccttgatttt
     9541 tctttggtta ctttctttgc atcaaggcaa agaaagtaac agcttggaat tcgacgccaa
     9601 acatatatgc cattgaaaca aatttcgttg cttgcctgat acgttttagg ttgacataac
     9661 ggtacttagc gtttattttg cgcggcgatg gaatctccga tcctaactga aacgccgcgt
     9721 ctgttgattc gccccatgca cgcatcggat gcggaaatgt tctttgattt attcggctcc
     9781 gcggaaacgc tgatccattt tccgcgtgcg tacacccgtg acgaagtgca tcggttgatc
     9841 cgtcgccagc aagaacgcta cacatcacgc ggttatggcc tctggacttt ggtggcgcgc
     9901 gactccggag agattatcgg ggattgcggg ttgatacctc agcgtgtcga tgcatgcgag
     9961 gaagtagaac tggcttacca tctccgcaaa aaattttggc atcagggtta tgccacggaa
    10021 gccggtgccg cggccatcgc atgggtcgaa gcccataacc atgccgcttc gctcatcgca
    10081 ctgatcaggc ccgaaaatgt acccagccgc catgttgcag aacggcttgg ctttataaaa
    10141 gacacggcga tttttcacgc cggactgctg catgacgtat ttcgtcgtgc gatagttcca
    10201 cagccggtga gcgtataatt ttttgacata acattattgc attatccatt ttatacccgt
    10261 tacaacaccc atgccttcat tacaactttt tgacacgtac acccgcgcat tgcggaattt
    10321 tgaaccgctc caacccggcg aagtcggcat gtacacatgc gggcctacgg tttatgacta
    10381 cgcgcacatc ggcaatctac gcacttacat tttcgaagac attctgcgac gtgtacttga
    10441 atacaacgga tacaaagttc atcatgtcat gaatatcacc gacgtcgggc atctcacatc
    10501 ggacgcggat accggcgaag atcgcatgga aaaaggttcc cgtcgtaccg gtaaaacagc
    10561 atgggaaatc gccgcggaat atacggaaga attcaaaaaa gatatgaaac ggttaaatat
    10621 aaccgagcca catacttggt gcaaagcgac cgatcatata ccggaacaga tcgaatttat
    10681 tcgcggcatc gaaaacaaag gatacaccta ccgcacatcg gacggcattt attttgacac
    10741 atccaaactc gatgactacg gatatatcgc gcggctcaaa gtcgaaggcc tcgaagcggg
    10801 ttcgcgcgta gagatcggcg aaaaacgcaa tatcaccgac tttgcgttgt ggaaattcag
    10861 caaacccgaa gagcagcgtc agatggagtg ggacagcccg tggggcaaag gttttccggg
    10921 atggcatatc gaatgttccg ccatgtcggc gaagtatctc ggttcatact ttgacattca
    10981 ttgcggcggt gaagatcata tcatggtgca tcaccccaat gagatcgcgc agacgcaggc
    11041 ttgttacgga acgcgtttgg ccaatttttg gatgcacggt tattttcttc agattgataa
    11101 ttcgcgtatg ggcaaatccg tcggcaattt tttacgtgtg cagacactca ttgatcaggg
    11161 atacgatccg atcgtgtggc gcatgttttg tctcagcgcg cattaccgta ccaaactcaa
    11221 ttttaactgg gaaagcctcg acggttcggc aacggcgcta caacgtctgc gcacggcagt
    11281 gtacgaatgg ggcgcggcag gcacaccgga tgaagcgacg ctcacgcagt ttcgcacgtt
    11341 tatcaatgat gatctcaata ttccccgtgt actcgcgctg gtgtgggacc ttgtaaaaag
    11401 cgacaaaact tcggctacaa aaaaagcgac cgtactggaa atggatcgcg tgctggggct
    11461 cggtttggcg gcgtgggttc ctgcggtcga agagattccg gccgaaatcc aagcttgggc
    11521 cgatgcccgc gtactggcac gcaaagaaaa acgctgggcc gatgccgaca cgtttcgcga
    11581 taagatcaca gccgcgggtt acgatatcaa agataccccg caaggcatca aagtcgaaaa
    11641 acgtaaaaca ccgggcgcct gatgaatata gataacgtca acatacatga ttttcccgtc
    11701 tcgctggctc cgatggaaga tgtcaccgac attgcctttc ggatcgtatg caaaaggctt
    11761 ggcgccgata ttctttatac ggagttcact tccagcgaag ccttgatccg cgatgcccgc
    11821 aaagctttac gcaaaatcca agtcgccgac gaggaacgcc ccgtagccat acagcttttc
    11881 ggcggattgg atacgtccat ggaaggcgcg gcgcggattg ctgaaaaagc caatccggat
    11941 tttatagata tcaactgcgg ctgctgggtc aaagatgtcg ccatgcgcgg cgccggggcc
    12001 ggtctgctca aagatctgcc gcgcttcgaa gcgatcgtac gctccaccgt gcggggcacg
    12061 caacttccgg tcaccgtaaa aacccggctg ggctgggaca aaaatacgat caacattctc
    12121 gatgtggcgc gtatggtcga agacgcgggc gcgaaagcgt tgaccgtgca ctgccgcaca
    12181 cgcgatatgg gacacgacgg ttccgccgac tggcattggt tggaaaaaat aaaaaaagtg
    12241 atttcgatac ctttattcgg caatggcgat gtcaaaacgc cgcaggatgc caaacgtatg
    12301 ctggagaccg gttgtgacgg cgtgatgatc ggccgagcgg ctatcgtcaa tccgtggatt
    12361 tttcgcgaat cgcggcacta catagatacc ggcgaaatcc tgcctccgcc cgatgccgaa
    12421 gagcggttac gggtttgtat cgaacatctg cggttatctg tcaaatacaa agaagaaaaa
    12481 tacggtgtgc tggaatttcg taaatattac tccggttatc tcaaaggcct tccctatatc
    12541 ggcaaactgc gcgccgaact gatgcagtac acgacactgc aacccattct cgatcgcctc
    12601 gcccaatacg gcgaagagat caaacaatgg caagcttccg aaggacgcga cgccatgctt
    12661 ccgcaactcg gcggcctcat cggcacgcgg catgagacgg tatgagtaag tatatttttt
    12721 attggtaaat tttctatcgg attcacgcga tgagtaccaa attttttaca aacgaagaag
    12781 agaacacgct tctcaccaag ctcgaaagca tctttcacca tcgaaatatc tactattttg
    12841 atgcactggt gggatttttg tgaagtgaaa taggcttacc gtcacctttg gtgttacttt
    12901 tttaatttac acttagtgtt tattataatt gactttttaa gtcatggtta ttacatttaa
    12961 aaacaaaaaa atatcagcac tcgttaataa cgacagaaag cttttgaagg agatgggtaa
    13021 aaagcgagct gacaaaattc gtcaacgcct tgatcaactt caagccgccg aaacattgga
    13081 ggacttacgc catgtggccg gcaactatca cgaactcacg ggtgaccgta aagggcaatg
    13141 ggcttgcgat cttgatcagc cctatcggct gatctttata ccgcacgaaa acccagtacc
    13201 ggttaataca tcaggtcagt atatatggct ggagattcgg ggtgttgaga ttattgaaat
    13261 cactgactat cattgaaaag aggacttatg attagagcca atgagtattt tccccaatcg
    13321 gtcacccatg ccggggctga tcttgatgaa aaactcaaag aactcggtat gggagtgaag
    13381 gaatttgcta tccgtacaga caaacccgaa aaaacgatca ttgccgtgat caaaggcgat
    13441 agcgccatca cacccgatat ggctgttcaa tttgaacagg tcttaaaaat cccggcgcat
    13501 ttttggctta atcgccaacg gcaatacgac gaatctatcg ctagatcgaa acagaaagaa
    13561 atgatccaag aagcggaaga ctgggcgtca agctttccct tgaaagaaat gttaaaatta
    13621 aaatggatac gttctttctc aagcaaatcg gaagcggtaa ctgccctctt tgaatttttt
    13681 ggaatttcga gcccggcggc atgggagagt ctgtatctaa ggtcagaact gaaagtccaa
    13741 ttccgcattt cgcttgccca taccaaagaa catcatgccc tttccgcctg gttgagacgc
    13801 ggggaaagac aagcgggaga actggatgtg ccggattatt ctgaaaaaaa ctttaaacag
    13861 gcattgccgg aaatcaaaac agtcatggcc aaacatccga aggatttttt tacaaaattg
    13921 caatcgctct gcaaagaagc cggggtcaaa gtcgtccata ccccatgtct tcccaaaacg
    13981 ccgattagcg gcgcgacacg ctggcttaat gacaccccgc tgattcagat gtccgggcgg
    14041 tacaagcgca atgacagttt ttggttcact ttctttcacg aggcaggaca tattcttctt
    14101 cacgggaaaa aagatatttt tctcgaaaat gtacaatacg gagatcgcga tgatgaaaaa
    14161 gaagaagaag ccaatttctt ttcacagaaa tgggttttta gcgaatcaga aaaaaccaat
    14221 gttttatcct atgctgataa aaccggtaga ttaacacatg cagatgttat tgaatttgct
    14281 aaggaattca acacccatcc tgcaatgatt ataggccggc ttcagcatga ccaaaaaatc
    14341 ccctatagct tgggtcggga atttatcaag ccggtagaat tagacaaaaa ctcggattgc
    14401 tgatctcaac gccaatcctc atttttttga cgctcactct cgttgcatcc ttttttttca
    14461 taacgtgcaa atcatcggat cgcgctgaat gcgatagttt cgtttatccc cttacacatc
    14521 cgtcggcacg ctggacccgg tacgtgccgc gccgacactt gccgctacga ccgcgcctat
    14581 cgcaatgcat tgggacagcg taaggtattc gtcgagcagc aaaaaaccga tcaccgcagc
    14641 gtacgcaggt tccatactca tcagtatgcc aaaaactttg ggaggcatac ggcgcagcgc
    14701 ttcgatttca aaactgaacg gaatcaccga ggacaaaaac gcaaccacta aagcaaaagg
    14761 tagtacggga acgatattga gccactgggg cgcgtcggcg attttgacgg gtatcagtac
    14821 caaactgcct acacacatcg cgatcgtgag accgacggag cccgaagtcg cactgcccaa
    14881 cttctcaccg agtatgatgt acaaacccca gcatgcaccc gccacaagcg ccaatacaaa
    14941 accgaccggg tccacatgcc cgctaaacgg attgagcaac gcgatgccga gcgcggctag
    15001 cgccacccag ataaaatcta gagcacg
//
LOCUS       DAOQGF010000083        15010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1834_length_15010_cov_6.640254, whole genome shotgun sequence.
ACCESSION   DAOQGF010000083 DAOQGF010000000
VERSION     DAOQGF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1834_length_15010_cov_6.640254"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK937_11280"
     CDS             complement(<1..675)
                     /locus_tag="PK937_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH33197.1"
                     /translation="MKLTLGLIALWLSIGGAWALAQIQAKKTTVAVLDFQSSGGISTD
                     EVKTLCNRFRGLLVKTSAFDVLEREKMGEILKAQDFNMSDACNTQACAVEVGKLLGVE
                     AMIAGDIGKIGETFTIDLRMIDVGTSKILSTQTRDFQGRIDGLLATMTSIAASFAKVD
                     KDAVVEVGDIYIASNPPGAEILINGKPISDVTPKLMENMTAGSYHIELRLGDLRARKT
                     VDIKGAT"
     gene            complement(854..1312)
                     /locus_tag="PK937_11285"
     CDS             complement(854..1312)
                     /locus_tag="PK937_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33198.1"
                     /translation="MKFFKIGSYILLSTTFIHLLGHFQERIPANEAEKQLFELAQNYK
                     FEIMGSTRSLMDFQNGFSLFFALFCLFVAVLNLFIASRVADRVEVMRGVLKINIAGMA
                     IATGICIVYFFAAPLTFMTLPLVFLCYCLFKAWEVKKFYCVCLTTKKFMT"
     gene            complement(1366..2319)
                     /locus_tag="PK937_11290"
     CDS             complement(1366..2319)
                     /locus_tag="PK937_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNH33199.1"
                     /translation="MLFSVATLWSQILPENRLVHTYSIVARDPETGDMGVAVQSNWFS
                     VGSLVTWAEAGVGAIATQSFVNPSFGPRGLELLKQGKTAQEALKILIDSDEGRAVRQL
                     SIVDAKGNVAAHTGEKCIPAAGHALGEQFSVQANLMLNDKVWPAMHKAFKEAQGPLAE
                     RMVAALEAGQSVGGDIRGKQSAAILVVKAKATGKIWEDRMIELRVEDHAEPVLEIRRL
                     LKMYRAYELMNKGDLAIEHHEMDKAMALYGGAEKMFPDNLEMQYWHAVALANAGKMSD
                     ALPMFKKIFAKDNNWRILTERLPGVGLLTVKPEELKSILLQ"
     gene            2609..4654
                     /locus_tag="PK937_11295"
     CDS             2609..4654
                     /locus_tag="PK937_11295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNH33200.1"
                     /translation="MRTLRFFMHALLCITALSYFACADKAPMIKGKTIEAENLDTTVA
                     PCNDFYQYANGGWLKNNPIPAAFSRWGSFNELADFNTTALRGLLETAANDKNAQKGSN
                     QQKIGDFYYTGMDSAGIEAAGLKPLASEFARIDGIKDVKDLAVVLSYYHQFAMDPAFN
                     VFVGQDDKNSTMMIANAYQGGLGLPDRDYYTKTDEKSKTIRSAYEKYIAKLLMLSGID
                     EKSSVKAASAIMGIETQLAKASMTRVEQRDPNAVYHKMTVEELEKTAPNFMWKTYFES
                     LGLAAMKDLNVGQPHFFKELSGMIKSVSIADWKSYLRFHVLNYAAPYMSSDFVNASFE
                     FQGKTLTGQKEMQPRWKRVSNVTDQSLGDALGQVYVEKYFPPASKKKALAMVNNVKEA
                     FRDRLMKLSWMSDSTRQKALAKLNTFVVKIGYPDKWKDYSTLEIDRQSYLGNVMRAQQ
                     FESKRLLAQVGQPVDRNRWGMTPPTVNAYYNTGMNEIVFPAGILQYPFFNPDADDAFN
                     YGSMGAVIGHEITHGFDDQGSQYDAEGNLKNWWSQEDRKQFESRADRVVKQFDGFVAI
                     DSMHVNGKLTLGENIADLGGLSIAYDAMIKAQNGKTPPKIGGFTAEQRFFLAWAQIWR
                     TNIRDERLRLMINTDPHSPGKFRVNGPLENMPEFYKAFGCKESDGMVKTDSSRAQIW"
     gene            4770..5345
                     /locus_tag="PK937_11300"
     CDS             4770..5345
                     /locus_tag="PK937_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNH33201.1"
                     /translation="MEQHHDTIVGEIWKQLRENPTYFISNMGRVKSYKYDQINGRILK
                     PRISGKNHLVVSLRRNNIKQSVYIHHLVAQYFLPPPAPDQKLVFHINGDYNNNQATNL
                     MWGTHKDHLLRSSQKNPRVHYYFFSNRVSRYDTGKITAADAEDIRKMLKNGLTGRRIA
                     KMYGISEMQVTRIRRFENWNPDKKNAQPLAS"
     gene            5474..8137
                     /locus_tag="PK937_11305"
     CDS             5474..8137
                     /locus_tag="PK937_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH33202.1"
                     /translation="MIDIPFEKHILDNGLTVILHEDHKAPVVAVNVWYHVGSKNEKPG
                     KTGFAHLFEHLMFNGSEHMDVDYFQVMENIGATNLNGTTNEDRTNYFQNVPVSALDIA
                     LWMESDRMGHMVGAITQAKLDEQRGVVQNEKRQGENEPYAVEEELIVQATWPVGHPYS
                     WTVIGSMEDLDHASLEDVHAWFRNYYGAANAVLVIAGAIDPKDALTRAKKYFGEIPSG
                     PPVSRYKSWIARRSGRQRAETHDRVPLAKITKVWNIPEWGTTEAHHLDLISDILGSGK
                     NSRLYKRLVYDEMLATDVNAYVDLREIAGQFTITAKARPGIDIAQLEKAIDEELVKLC
                     TEGPSEKELTRVRTQFFAGFVRGIERIGGFGGKSDILARGEIFSSNPQAYKKNMHDVE
                     SATTEKLRDTARHWLSDGDYTLTTKPFPEFQITDTAADRNRLPEAGVPPEVRFSKMER
                     HTLANGLKIILVPRHSVPVINMSLVLDAGYASDALSIPGTASLAMAMLDEGTTGRSSL
                     EISESLAMLGSTLTTGSSLDVSTVNLSTLKNNLSPSLDIFADVICRPIFPEQDFLRLR
                     EQQRAGIRQEKSSPVPMALRVLPQLLYGPSHAYGIPFTGSGTESALDRITRDDLKRFH
                     QTWFHPNHTTLIVVGDTTADVIIPLIENKLGAWQSGTTPQKNVVPVKIPDAPVVYLMD
                     RPGSQQSTIFAAHIAPPTNNPDEIAIQTMNNILGGDFTSRINMNLREDKHWAYGAFSF
                     LWDARGQRPFIAYAPVQSDKTKESVAEIRKEISDFITIKPLSEEEFMRTQSNQVLQLP
                     GTWETNNAVLGSLQQLVQFGFNDDYFDHYAQSIRNLSPADARKAAQSLLKPDHLIWVI
                     VGDRKQIENEIRSLNIGVIRYIDSDGNVLES"
     gene            complement(8372..9013)
                     /locus_tag="PK937_11310"
     CDS             complement(8372..9013)
                     /locus_tag="PK937_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNH33203.1"
                     /translation="MYELIAFGLLCFTSFFSVINPLGVMPVFLTMTADLDAAHRNSTA
                     RKAVLVAVISMVGFALSGQLLFKFFGISVDSFRVVGGVIFFMMGYEMLQARLSRVEID
                     EESVKSYVEDISITPLGIPMICGPGAITTSIVLMEDAKTFEMKAVLLGVILLIGLITL
                     IGLLGAGRFVNILGETGNKILMRLMGLIIMVIAVEFFFAGIKPFVVRMMQTVG"
     gene            complement(9050..10729)
                     /locus_tag="PK937_11315"
     CDS             complement(9050..10729)
                     /locus_tag="PK937_11315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH33204.1"
                     /translation="MRSLITIFLTISCTLYAQVLDNDETVTRADSLRGMLTPLRTCFD
                     VTHYALDVKIDTTTRRISGSNTITLVAMQDFTRLQIDLFDHYAIDRIEWKGKKLKFSR
                     EFNAVFVEFPETIRKSSRQSFTVFYSGTPIVAKNPPWDGGFIWSRDKSGNPWIAVACQ
                     GFGASSWWPNKDHQSDEPDSMLISITVPKGLTNVSNGRLRKQKDVGSGWTRFDYAVTY
                     PINNYNVTFNVGKFAYFGETYTSYDGQKLTCDYYVMPYNLEKAKVQFAQVKGMMDAFE
                     KSFGKYAFYRDGYKLVESPHLGMEHQSAVAYGNNYVNGYKGTGQAAEGLLFDFIIIHE
                     TAHEWWGNAVGSNDLADMWIHESFGNYAETIYLEGVYDYPTSLRYINANKTLVKNDRP
                     IIGPRGVNKEGSGDMYPKGGLMLNTLRSVINNDKLWFEILRGIQDTYRYKTIDADSIF
                     NYVRLKTGTDYDYFFDQYLKHKALPKLDVALFKKGSTTTMRYRWTADVKSFRMPIRVT
                     TTKDRYEWVTPTTEWQMMKLENLDPRDFKIEEDRFYVDKNIYWMFLDERLQ"
     gene            complement(10771..12156)
                     /locus_tag="PK937_11320"
     CDS             complement(10771..12156)
                     /locus_tag="PK937_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNH33205.1"
                     /translation="MKTFFSHILDGIRGTEHDYTEGSLGKAITLLSIPMVLEMFMESL
                     FGIVDVYFVSRLGPDAVASVGLTESMLTIIFAVAIGLSMATTAMVARRIGEHNPEAAS
                     IAGFQAVLIGVAVSLPVSVIGLTMTPWLLEQMGASAGIIENGTGYAVWMIGGNLTIVL
                     LFLNNAIFRGAGDAVTAMHALWVANLLNMVLDPIFIFGFGPVPAMGVTGAAIATNIGR
                     GVGVLFQLYILIYGKRRLVFSPAVMRLDPEVLWNLIKVSVGGIFQFFITTASWLGLVR
                     IVAMSGSAALAGYTIAIRVIIFAIMPSWGMSNAAATLVGQNLGAQKPDRAERAVWFTA
                     RANMFFLGVVAILFISFAEMIIAWFTDDSQVVYYAVDCLRIISYGYVVYACGMVMVQA
                     FNGAGDTRTPTWINLGCYWMFQIPLAYTLAVIYGFGAQGVFAAIPIAESVATVVSVIM
                     FRKGHWKLQKI"
     assembly_gap    12166..12265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12390..14870)
                     /locus_tag="PK937_11325"
     CDS             complement(12390..14870)
                     /locus_tag="PK937_11325"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNH33206.1"
                     /translation="MFDLDMIKRVYAGMKAKQDRARKLFGRPLTLTEKILVSHFAENP
                     KAAPARFKDYVDLAPDRVAMQDATAQMALLQFMSSGKAKVAVPSTVHCDHLIQAEVGA
                     ADDLKKANSDNKEVYDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSH
                     TPNAGGLGMIAIGVGGADAVDVMAGLPWELKWPGITGVKLTGSLSGWTSPKDVILKLA
                     GILTVKGGTGKIVEYFGPGTASISCTGKGTICNMGAEIGATTSIFPYDMRMAAYLRTT
                     ERAAVAKLADQYAALLTADEGSEAYYDEVIEINLSELEPHVNGPFTPDKAWKISELKQ
                     AVADNNYPDEIRVALIGSCTNSSYEDIERSASLARIASQHGLKAKAEFVITPGSEQIR
                     ATIERDGQLEDFAKIGGKVLANACGPCIGQWKRHGVSEGDPNTIVTSYNRNFKARNDG
                     NSGTHAFVASPEIVTALAIAGRLSFNPMTDTITNAEGQQIRLLPPDGDELPRNGFTPG
                     ESGYVTPAKDGSKVQVIIDPASSRLQALSPFTAWDGRDIENAVVLLKAKGKCTTDHIS
                     PAGPWLKFRGHLDNISNNMFIGAVNAFTGEAGKGKNVITGETKGYSDNARYYKSQSIS
                     WITIGDENYGEGSSREHAAMEPRFLGGRAIIVKSFARIHETNLKKQGMLALTFKNPAD
                     YDKIREDDRLAIRGLTAFTPGVPLTMDIKHNDGSVETIELNHSYNAQQIGWFKAGSAL
                     NLIGGKTAASSRKKTATKTKKAAKKKTVIKEAKAKTKTKKSAKVAPKKQAAKSAAKKK
                     TKKAVKKTIKKATKKTNRKPVKKTSRKR"
BASE COUNT         4113 a         3684 c         3325 g         3788 t
ORIGIN      
        1 ggtagctcct tttatatcaa cggttttgcg tgcccgcaga tcaccaagtc gaagttcgat
       61 atggtaacta cctgcggtca tgttttccat tagtttgggc gtcacatcgc tgatgggctt
      121 gccattgatc aggatttccg ctccgggagg attggacgct atatagatat cgccgacttc
      181 gacgaccgcg tctttatcta ctttggcaaa tgaagcggcg atcgaagtca tggttgccaa
      241 aagcccgtca attctgcctt ggaaatcacg ggtttgcgtg gataaaattt ttgacgtccc
      301 aacatcaatc atgcgcagat cgatcgtaaa tgtttcgccg atttttccga tatcccctgc
      361 tatcatcgct tcgaccccca gcagtttacc gacttccaca gcacatgcct gggtattgca
      421 agcatccgac atattaaagt cttgcgcttt gagtatttca cccatttttt ctcgttcgag
      481 aacatcaaac gcactggtct taaccaagag ccctctaaac cgattgcata acgtcttgac
      541 ctcgtctgtg gatataccgc cgctactctg aaaatcaaga acagcaacag ttgttttttt
      601 ggcctggatt tgcgccaaag cccaagcgcc tccgatagaa agccacaaag cgatcagccc
      661 gagggttaac ttcatacgaa gattcccaat tttaaggtta aaaattatta aagattggaa
      721 gcacaaaccg acgcctatga atgagagacc cgcatggttt gtaaacgaag taagagtaaa
      781 aaactaggcg ctaacaagct ttttatagtg agtatgtaca atatgacact aatttcacag
      841 cgctaaaaat gatttatgtc atgaatttct tagttgtcaa gcagacacaa taaaatttct
      901 ttacctccca agccttaaaa aggcaataac ataaaaaaac gagcggcaag gtcataaatg
      961 tcagcggtgc agcaaaaaaa tacacgatgc atatacccgt ggctatcgcc ataccggcta
     1021 tattgatttt caaaaccccg cgcatcactt ctacccgatc ggccacacgt gaagcgataa
     1081 aaagatttaa aacggcgaca aacaaacaaa aaagcgcgaa aaacaaacta aatccgtttt
     1141 gaaaatccat cagcgaacgt gtactgccca tgatttcaaa tttataattt tgagccagtt
     1201 caaatagttg tttttcggct tcgttggccg gtattcgttc ctgaaaatgc ccgagcaggt
     1261 gaataaaggt cgtagacagt aggatatatg aacctatttt gaagaatttc atacgactga
     1321 acttcctttc tatacttaga aacgattatc gccacagatc aaaaactatt gtaaaagaat
     1381 agacttcaat tcctccggtt tgactgtcag caaacccaca cccggtaaac gttccgtgag
     1441 aatacgccaa ttattatcct tggcgaatat ttttttgaac atcggtagcg catcgctcat
     1501 tttaccggcg ttggccagcg cgacagcatg ccagtattgc atttcaaggt tatcgggaaa
     1561 cattttttca gcgccgccat acagagccat cgccttatcc atttcgtgat gctcgatagc
     1621 caaatcgcct ttattcatca gttcgtaagc gcgatacatt ttgagaagtc ggcgtatttc
     1681 gagaaccggc tcggcatgat cttcaacgcg taactcgatc atccggtctt cccagatttt
     1741 cccggtagct ttcgccttaa cgacaagaat ggccgcagac tgtttaccgc gaatatcacc
     1801 tccgacgctt tgcccggctt ccagtgcggc aaccatccgt tcggcgagcg gcccctgcgc
     1861 ttccttaaaa gctttatgca tcgccggcca tactttgtca ttgagcatca gattcgcctg
     1921 tactgaaaat tgttccccca acgcgtgacc ggctgcggga atacattttt cgcccgtgtg
     1981 ggcggcaaca tttcctttgg catctacgat agataattgg cgaacggcgc gaccttcgtc
     2041 ggaatcaatt aatattttaa gcgcttcctg cgccgttttg ccttgcttca agagttccag
     2101 tccgcgcggc ccgaacgacg gattgacaaa cgattgtgtg gcgatggcgc cgacaccggc
     2161 ctccgcccaa gtcacgagcg atccgacgga aaaccaattg gactgcaccg cgacgcccat
     2221 atcaccggtt tcgggatcgc gcgcgacgat cgaatacgtg tgtacgaggc gattttcggg
     2281 taaaatctgc gaccaaaggg ttgcaacgct aaaaagcatt aaccaccagt gtttcatggg
     2341 atttccttcc aaaaaaaatt gagcgatgac ggatgagata tcgaaatgcc gatcaatata
     2401 ccggatatgt tagtatttcg caagcattgc gcgatctcgt ttctttgtta aagaaaaatc
     2461 aacatacatc cgagcaggcc cccatccata tccgaagtcg aaatttgcag ctcaaatttt
     2521 atactataat tttttgcatt tatctcaggc tttgcctaca attgctggcc taaaaaatta
     2581 catccctaat aatcggagta aaccctttat gcgaacgtta cgttttttta tgcatgcatt
     2641 gttatgtata accgcgctgt cttatttcgc ctgcgccgat aaagcgccga tgatcaaagg
     2701 taaaaccatc gaagcagaaa atttggacac cacggttgca ccgtgcaacg atttttatca
     2761 gtacgccaac ggcggttggt taaaaaacaa tcccattccg gcggccttca gccgctgggg
     2821 cagcttcaat gaattagccg attttaatac aacggcgctg cgcggtttat tagaaaccgc
     2881 agcaaacgat aaaaacgctc agaaaggttc caaccaacaa aaaatcggtg atttttatta
     2941 taccggtatg gatagcgccg gtatcgaggc tgccgggtta aaaccactgg catccgaatt
     3001 tgcacgcatt gacggcataa aagatgtcaa agatttagcc gtcgttttat catattacca
     3061 tcagtttgcg atggatccgg cgttcaatgt ttttgtcgga caagacgata aaaacagtac
     3121 catgatgatc gcaaacgcgt accagggcgg gctcggttta cccgatcgcg attattacac
     3181 caaaacggat gaaaaaagta aaaccattcg ttcggcctat gaaaaataca tcgccaaact
     3241 tttaatgtta tccggtatag acgaaaaatc atccgtcaag gccgcatcgg cgatcatggg
     3301 catcgaaacg caattagcca aagcctccat gacccgcgtc gaacaacgtg atcctaatgc
     3361 cgtgtaccat aagatgacag ttgaggaatt ggaaaaaacg gctcccaatt tcatgtggaa
     3421 aacttatttt gagagtcttg gtcttgccgc aatgaaagac ctcaacgtcg gccaacccca
     3481 ttttttcaaa gaactcagcg gcatgatcaa atcggtatcc atcgccgatt ggaaatctta
     3541 cctacgtttt cacgtactga actatgcggc gccgtatatg agttcggatt ttgtgaatgc
     3601 ctcgtttgaa tttcagggaa aaacgctgac cggacaaaaa gaaatgcaac cgcgctggaa
     3661 acgtgtcagc aacgtcaccg atcaatcgct cggcgatgcg ctcggtcagg tgtatgttga
     3721 aaaatatttt cctccggcat caaagaaaaa agccctcgcg atggttaaca acgtaaaaga
     3781 agcattccgt gatcgcctga tgaaactaag ctggatgagc gacagcacac gtcagaaagc
     3841 gctcgccaag ctaaatacct ttgtcgtaaa aatcggttac ccggacaaat ggaaagatta
     3901 ttcgactctc gaaattgaca gacaatccta tctcggcaac gtcatgcgcg ctcaacaatt
     3961 tgaatccaaa cgattgctgg cgcaagtcgg acaacccgtg gatcgtaatc gctggggcat
     4021 gactccgccg accgtcaatg cgtactacaa tacgggcatg aatgaaatcg tttttcccgc
     4081 cggtattttg caatatccat tttttaatcc cgatgccgac gatgcattta actacggttc
     4141 gatgggcgcc gtgatcggtc atgagatcac acacgggttt gatgatcaag gcagccagta
     4201 tgacgctgaa ggcaacctca aaaactggtg gtcccaagaa gaccgcaaac aattcgaatc
     4261 ccgtgctgac cgcgttgtga aacaatttga cggttttgtc gcgatcgact ccatgcatgt
     4321 aaacggtaaa ctcacgctgg gagaaaatat cgccgacctc ggcgggttgt ccatcgctta
     4381 cgatgccatg atcaaagcac aaaacggaaa aacgccgcct aaaatcggcg gattcactgc
     4441 ggaacaacgt ttcttcctcg cttgggcaca aatctggcgg accaacatac gcgatgaacg
     4501 cctgcgtctg atgatcaaca ccgatccgca ctcgcccgga aaattccgcg tcaacggccc
     4561 gctcgaaaac atgcctgaat tctacaaagc tttcggatgc aaagaaagcg acggtatggt
     4621 aaaaaccgac agcagtcgcg cacaaatttg gtaaataaaa aatataacaa gaggtgagta
     4681 actttattca cctcttgtta tctatataga tttttattag tatccgatac cgtctaataa
     4741 tttttataaa cgaaaggtca taaatcctga tggaacaaca ccacgatact atcgtggggg
     4801 agatttggaa acaattgagg gaaaacccga cgtattttat cagtaacatg ggtcgggtaa
     4861 aaagttataa atacgatcaa atcaacgggc gtatactgaa accacgtatc tccggtaaga
     4921 atcatttggt agttagctta cgtaggaaca atatcaaaca gtccgtttac attcatcatc
     4981 tcgtcgcgca atactttttg ccgccgcctg cacccgatca aaagttagtt tttcacatca
     5041 acggcgatta taataacaat caagcaacca acctgatgtg ggggacgcac aaagatcatt
     5101 tattacgttc ttcacagaaa aacccgcgcg tacattatta ttttttctca aatcgggttt
     5161 cgcgttacga tactggcaaa ataacagccg ccgatgccga agatattcgc aaaatgctta
     5221 aaaacggctt aaccggacgc cgtattgcca aaatgtacgg tatcagtgag atgcaagtaa
     5281 cgcgcattcg tcgttttgaa aattggaacc ccgataaaaa gaacgcacaa cctttggctt
     5341 cgtgagatta tatcttaata cgttaaaaag cgcggtcgca tccgcgcttt ttttttgcgt
     5401 tgaaaatatg atcccgttta gagatactca ttcaccattt atttgaaaaa ctaatacgcg
     5461 agcgataaaa tccatgattg atattccttt tgaaaaacac attctggata acggattgac
     5521 cgtaatttta catgaagatc acaaggcgcc cgttgtcgcg gtgaatgtgt ggtatcacgt
     5581 cggatccaaa aacgaaaaac cggggaaaac aggttttgcg catctttttg aacatctgat
     5641 gttcaacggc agcgagcaca tggatgtgga ttatttccaa gtcatggaaa atatcggcgc
     5701 cacgaatctg aatggtacca ccaacgaaga tcgcaccaat tacttccaaa acgtacctgt
     5761 atccgcattg gatatagcgt tatggatgga gtccgatcgt atgggccaca tggtcggcgc
     5821 gatcacgcaa gccaagcttg acgaacagcg cggtgtggta caaaatgaaa aacgtcaggg
     5881 cgaaaatgaa ccgtatgccg tcgaagaaga attgatcgtg caagccacat ggccggtcgg
     5941 tcatccctat tcatggaccg tcataggttc tatggaagat ctggaccacg catcgctcga
     6001 ggatgttcac gcttggtttc gcaattatta cggtgcagcc aatgcggtgc ttgtcatcgc
     6061 cggcgccata gatcccaaag atgctttgac gcgcgctaaa aagtattttg gcgaaatacc
     6121 ctccgggcct cctgtttcgc gatacaaatc atggatcgcc cgtcgcagcg ggcgccaacg
     6181 cgctgaaacc catgatcgtg tgccgctcgc caaaatcacc aaagtatgga atattccgga
     6241 atggggcacg accgaagccc atcatctgga tctgatcagc gacatcctcg gcagcgggaa
     6301 aaattcacgt ttgtataaac gtttggtata tgatgaaatg ctggccaccg atgtcaatgc
     6361 gtacgtagat ttgcgtgaaa tcgcaggcca atttaccatc acggccaaag cacgccccgg
     6421 gatagacatc gctcaattgg aaaaggctat tgatgaagaa ttagtcaaac tatgcacgga
     6481 gggcccttcc gaaaaagaac tcacacgcgt acgcacacaa ttttttgcag gatttgtgcg
     6541 cggtatcgaa cgtatcggcg gatttggcgg caaatcggat atattggcgc gcggagaaat
     6601 ttttagcagt aaccctcaag cgtacaaaaa aaacatgcac gatgtcgaat ccgctacaac
     6661 ggaaaagctg cgtgatacgg ctcggcattg gctaagcgac ggcgattata ctttgacaac
     6721 caaaccgttc ccggaattcc aaataaccga caccgcggca gatcggaatc ggctgccgga
     6781 agccggtgtt ccgcctgaag ttcgtttttc aaaaatggaa cgtcatactt tggccaatgg
     6841 ccttaaaatc attttagtac cacggcattc cgttcccgtt atcaatatga gcctcgtgct
     6901 tgatgcggga tatgcgtcgg acgcattatc cattcccgga accgcaagcc tcgctatggc
     6961 catgcttgat gaaggcacaa ccggccgctc ttctctcgaa atcagcgaat cattggccat
     7021 gttgggcagc acgctgacga ccggttcatc gctggatgtc tccaccgtca atctttctac
     7081 tttgaaaaat aatctctcac cgtcgttaga catttttgcc gatgtgattt gccgtcctat
     7141 atttcctgaa caagacttcc ttcgtctccg tgaacagcaa cgcgccggta ttcgtcagga
     7201 aaaatcttca cccgtgccga tggctttgcg cgtattacca caattgctct acggaccatc
     7261 gcacgcgtat ggcatacctt ttacaggctc cggtacggaa tcggcgcttg atcgcatcac
     7321 gcgtgacgat ttaaaacggt ttcatcaaac atggtttcat cccaatcata ccacgttgat
     7381 cgtggtcggc gatacgacag cggatgtcat aattcctttg attgaaaaca aattgggggc
     7441 atggcaaagc ggcacgacac cgcaaaaaaa tgtagtgccg gtaaagatac ccgatgcgcc
     7501 cgtggtatat ctgatggatc ggccgggttc gcaacagtcc acgatttttg ccgcacacat
     7561 cgcgccgccg actaataatc cggatgaaat cgccattcaa acgatgaata atattctcgg
     7621 cggtgacttt acttcgcgta tcaatatgaa tttacgcgag gataagcatt gggcttatgg
     7681 cgccttcagt tttctgtggg atgcgcgcgg ccaacgtccg tttatcgcct acgctccggt
     7741 acaaagcgat aaaaccaaag aatccgtcgc ggaaatacga aaagaaatat ccgattttat
     7801 tacgataaag ccgctgtctg aagaagagtt tatgagaaca caatccaatc aagtactgca
     7861 actgccgggt acttgggaaa cgaataacgc ggtattaggt tcgcttcagc agttggtcca
     7921 gttcggattt aatgatgatt attttgatca ttacgcacaa tcgatcagaa atctttcacc
     7981 cgcggatgct cgaaaagcgg cgcaatcgtt attgaaaccg gatcatctta tttgggtgat
     8041 cgtcggcgat cgcaaacaaa tcgaaaatga aattcgttct ctcaatatcg gcgttattcg
     8101 ctatatcgac agcgacggaa atgttttaga atcgtaggta tgatactttc cgcgtgactc
     8161 agtacaaatc ggagggattg ttcgactaaa tctcgcgaat aaaaattgtt ttgatgacgc
     8221 ctcgcgtact tagcgtctta gcgcgtacgt atgccctctc gcaaagccgc aaagttaaaa
     8281 ataactcaac ccaagctcag ccacaaacgg ctaagaatga taccttccgc attgtcctgg
     8341 caggttcgat tacatctttc gcctggcaac cttatccgac cgtttgcatc atgcgtacga
     8401 caaacggttt gataccggcg aagaaaaatt cgacggcgat aaccatgatg ataaggccca
     8461 tcaggcgcat gaggatttta tttccggttt caccgagaat atttacaaag cgacctgcgc
     8521 ctaaaagccc gatcaaagtg atcaggccga tgagcaatat cacgccgagc aaaacagctt
     8581 tcatttcaaa cgtcttggca tcttccatta aaacgatgga cgtcgtgatc gctccggggc
     8641 cacagatcat gggaattcca agcggtgtga tcgatatatc ttccacgtac gatttgaccg
     8701 actcttcgtc aatttcgaca cggctcagac gcgcctgaag catctcatat cccatcataa
     8761 aaaatatcac gccgccgacc acgcgaaaac tatccacgga aattccaaaa aatttgaaaa
     8821 gcaattgccc tgacaaagcg aagccgacca tactgataac cgccaccagc acagccttgc
     8881 gcgccgtact gttccgatgc gcagcatcga gatccgccgt catcgtcaga aaaaccggca
     8941 tcacacccag cggattaatc accgaaaaaa aagatgtaaa acataaaaga ccgaaagcga
     9001 tgagttcgta catgcggcaa tagcctttac tttaatttat gacaagatgt tattgcaagc
     9061 gttcatccaa aaacatccag tagatatttt tatctacata aaaacgatct tcttcgatct
     9121 taaaatctct cggatccaga ttttccaatt tcatcatctg ccattccgtc gtcggcgtga
     9181 cccattcata ccgatcttta gttgtcgtta cacgaatggg catacgaaaa cttttaacat
     9241 ccgccgtcca gcggtatcgc atcgtcgtgg tcgaaccttt tttgaatagc gcgacatcga
     9301 gtttaggcag agccttgtgt ttgagatatt gatcaaaaaa ataatcgtaa tccgttccgg
     9361 ttttcaggcg aacgtaattg aagatagaat cggcatctat cgttttgtaa cggtacgtat
     9421 cctgaatacc gcgtaatatt tcaaaccaaa gtttatcatt attgattacg ctgcgcagcg
     9481 tattgagcat cagtccgcct ttgggataca tatcgccgga gccttcttta ttcacgcccc
     9541 gcgggccgat gatcggacgg tcatttttga cgagcgtttt attggcattg atatagcgca
     9601 gagacgtcgg ataatcatac acaccttcga gataaatcgt ttcggcgtaa tttccgaaac
     9661 tttcgtgaat ccacatatcg gccagatcat tggaacctac ggcgttgccc caccattcgt
     9721 gtgctgtttc atgaatgatg ataaagtcaa acaacaatcc ttccgccgct tgccctgtgc
     9781 ctttgtaacc gttgacgtaa ttattaccat acgctacggc gctttgatgc tccataccca
     9841 aatgcggtga ctcgactaat ttatacccgt cgcgataaaa cgcatatttg ccgaacgatt
     9901 tttcaaacgc gtccatcata cctttgactt gagcaaactg tactttggct ttttcgagat
     9961 tgtacggcat gacataataa tcgcatgtca acttctggcc gtcgtaggat gtataagttt
    10021 ctccaaaata cgcaaatttg cccacattga atgtgacgtt ataattattg atcggatatg
    10081 taacggcata atcaaaacgc gtccaaccgg agcccacatc tttttgtttt cgcaagcgac
    10141 cattggaaac attggtaagg cctttgggta cggtaatgct gatgagcata ctgtcgggtt
    10201 cgtccgactg atgatcttta ttaggccacc aactgctggc gccgaaaccc tggcatgcga
    10261 cagcaatcca cggatttccc gatttgtcac gcgaccaaat aaatccgccg tcccagggcg
    10321 gatttttagc gacaatgggt gtgcccgaat aaaaaacggt aaaactctga cgggagcttt
    10381 tgcgaatcgt ctcaggaaat tctacgaaca cggcattgaa ctcacgagaa aacttgagtt
    10441 tcttaccttt ccactcgatc cgatcaatcg cgtaatgatc gaataaatca atttgtaacc
    10501 gggtaaagtc ctgcatcgcg accaacgtga tcgtattgct tccgctgata cggcgtgtag
    10561 tggtatctat ttttacatcc agagcataat gcgtcacatc aaaacatgtt cgcagcggcg
    10621 tcagcatacc gcgcaaagaa tccgcacggg ttacggtttc atcgttatct aaaacttgcg
    10681 cgtacaatgt acagcttatc gtaagaaaaa tagttattaa acttcgcata acgtttttcc
    10741 tttggtatgt gatgagtgac aaaaaacaat tcatattttt tgcagtttcc aatgtccttt
    10801 tctaaacata atcacgctga cgacggttgc aaccgattcc gcgatcggta tagcggcaaa
    10861 cacaccctga gcgccaaaac cataaatcac ggcaagcgta tatgccagag ggatctggaa
    10921 catccaatag caaccgagat tgatccacgt cggcgtgcgt gtatcgccgg caccgttaaa
    10981 tgcctgcacc atcaccatac cgcaggcata cacgacataa ccgtagctaa tgatacgtaa
    11041 acaatccacg gcataataaa cgacctgtga atcatcggta aaccaagcga tgatcatctc
    11101 agcaaatgaa ataaaaagaa ttgccacaac cccgagaaag aacatattcg cacgcgccgt
    11161 gaaccacacc gcgcgttcgg cacgatcggg cttttgagcg ccgagatttt gaccgaccag
    11221 cgtcgccgcc gcgttactca taccccagga cggcatgatg gcaaaaataa tcacgcgtat
    11281 ggcgatagta taaccggcca aagccgcgct gcctgacatc gcgacgatgc gtacgagccc
    11341 aagccagctt gccgtcgtaa taaaaaattg gaaaatgcct ccgacagata ctttgatcag
    11401 gttccacaaa acttcgggat ccaatcgcat cacggcgggc gaaaaaacaa gccgccgttt
    11461 tccatagatc agaatgtata actgaaacaa cacacccacg ccgcgaccga tattggttgc
    11521 tatggccgcg ccggtaacac ccatggccgg cacgggacca aaaccgaaaa taaaaatcgg
    11581 atcaagaacc atgttgagca aattggcaac ccacaaagca tgcatggctg tgacagcatc
    11641 gcccgcgccg cgaaaaatag cattattcag aaaaagtaaa acgatggtga gattgccgcc
    11701 gatcatccat acggcataac cggttccatt ttcgataata ccggccgaag cgcccatttg
    11761 ctccaaaagc caaggcgtca tcgtcagtcc gatcacgctg accggtaagg aaacagccac
    11821 gccgatcagt acggcctgaa aacccgctat ggaagccgct tcggggttat gttcgccgat
    11881 acgccgagcg accatagctg tcgtcgccat actaaggcca atcgccacgg caaaaataat
    11941 cgtcaacatg gattcggtta acccgacgga agctaccgca tcgggaccaa gccggctgac
    12001 aaaatacacg tccacaatac cgaacagcga ttccataaac atttccaaaa ccatcggtat
    12061 ggataaaagt gtaatcgctt ttccgaggct gccttccgta taatcgtgtt cggtgccacg
    12121 aatgccgtcg agaatatggc tgaaaaacgt tttaatcgaa aatttnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnataaa ataaaaaagg ctgcatcata agctacgaca
    12301 tatcgctgtg tatttttcgg catgatgtac aaaccgctgc gatcgtagtc gtaaactttt
    12361 gatacagccc ggtaagggaa atgaaaagtc tatcgcttcc gtgaggtttt tttcacgggt
    12421 ttacggtttg ttttctttgt cgcttttttg attgtctttt taacggcttt ttttgttttt
    12481 ttcttagctg ctgacttagc ggcttgtttt ttgggagcga ctttggcgct ttttttggtt
    12541 ttggtctttg ctttcgcttc tttaattacc gtttttttct tggctgcttt tttggtttta
    12601 gttgccgttt ttttacgcga cgaagcagcc gttttgccgc ctatcagatt gagtgcgcta
    12661 ccggctttga accaaccgat ctgttgcgcg ttatacgaat ggttgagttc gatcgtctcc
    12721 acggaaccgt cgttatgttt aatgtccatc gtcagcggca cacccggtgt aaacgccgtc
    12781 aacccgcgga ttgccagacg atcgtcttca cgaattttat catagtccgc cgggttttta
    12841 aatgtcaacg ccaacatacc ttgttttttg agattcgttt cgtgaatacg tgcaaacgat
    12901 ttgacgatga tggcgcgacc acccaagaaa cgcggttcca tcgccgcatg ttcgcggctt
    12961 gatccttcac cgtaattttc gtcgccgata gttatccaac taatgctctg ggatttataa
    13021 taccgtgcat tatcggaata gcctttggtt tcgccggtaa tcacattttt acctttgccg
    13081 gcttcaccgg taaatgcatt aacggcgccg ataaacatgt tgttggaaat attatccaag
    13141 tgcccgcgga atttgagcca cggtccagcg ggagaaatat gatccgtcgt acatttgcct
    13201 ttggctttga ggagtaccac ggcattttcg atatcgcggc catcccatgc cgtaaaagga
    13261 ctcaatgcct gcaaacgcga actcgcggga tcaatgatca cctgcacttt ggaaccgtct
    13321 tttgccggtg tgacataacc actttcgccg ggcgtaaatc cgttgcgcgg caattcatca
    13381 ccgtcgggcg gcaataaacg aatttgttga ccctccgcat tggtgatcgt gtccgtcatc
    13441 ggattaaagc tcagacgtcc tgcgatggcc agtgccgtta ctatttccgg actggcaaca
    13501 aacgcatggg taccggaatt accgtcattg cgggctttga aattacgatt gtacgatgtg
    13561 acgatggtat tcggatcgcc ctcgctgacg ccatgacgtt tccactgacc gatgcacgga
    13621 ccgcaggcat tggctaaaac tttaccgccg attttagcga aatcttccaa ttgaccgtcg
    13681 cgttcaatcg tcgcacgaat ttgttcggag ccgggcgtaa tcacaaattc agctttggct
    13741 ttaagaccgt gttgcgaagc gatgcgcgct aaactggccg aacgttcgat atcttcgtat
    13801 gatgaattgg tacacgaacc gatcaatgcc acacggattt catccggata attattatcg
    13861 gcaacggctt gtttcaattc agaaattttc catgctttgt ccggtgtgaa aggcccgtta
    13921 acatgcggct ccaattcgct cagattgatt tcgatgactt catcgtaata cgcttccgac
    13981 ccttcgtcgg cggtaagcaa cgcggcgtac tgatccgcca gtttggccac ggcagcgcgc
    14041 tccgtcgtac gaagatacgc cgccatacgc atgtcatagg gaaaaataga agtggtcgcg
    14101 ccgatctccg cacccatatt acaaatcgtt cctttaccgg tacaggaaat ggatgccgtg
    14161 ccgggaccaa aatattcgac gattttcccg gtgccgcctt tgacagtcag aataccggct
    14221 aatttgagaa taacgtcttt gggtgaagtc catccgctga gcgaacccgt gagtttgaca
    14281 ccggtaatgc cgggccactt gagttcccac ggcaatcccg ccatgacatc cacggcatcc
    14341 gcgccgccga caccgatggc gatcatgcca aggccgccgg cattgggtgt gtgcgaatcc
    14401 gtgccgatca tcataccgcc gggaaacgca taattttcaa gcacgacttg atggataata
    14461 cctgcgccgg gtttccaaaa gccgataccg tatttattgg agacgctggc caagaaatca
    14521 tacacttctt tgttatcact attggctttt ttcaaatcgt ccgccgcacc gacttcggct
    14581 tgtatcaggt ggtcgcagtg caccgtggaa ggcacggcca ctttggcttt gccggaagac
    14641 ataaactgaa gcaacgccat ttgtgcggtc gcatcctgca tggctacacg atcgggtgca
    14701 agatctacat aatctttgaa ccgtgcgggc gcggctttag gattttccgc aaaatgggaa
    14761 acgagaattt tttccgtcag cgtgagcgga cgtccgaaaa gtttgcgtgc gcggtcttgt
    14821 ttggccttca taccggcata tacgcgcttt atcatatcga gatcaaacat ggcagtaaaa
    14881 ctccttgatc ataattttat gtacataaaa gtagaaagat tgttctgaat tggctgatgc
    14941 attattagtc ggtgagttat tcatgcgagc gcaaaataca taacttagaa agtttacgca
    15001 agggaatatc
//
LOCUS       DAOQGF010000084        14847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1856_length_14847_cov_6.491820, whole genome shotgun sequence.
ACCESSION   DAOQGF010000084 DAOQGF010000000
VERSION     DAOQGF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_14847_cov_6.491820"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PK937_11330"
     CDS             complement(<1..1413)
                     /locus_tag="PK937_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH33207.1"
                     /translation="MKSCFVVVFTFIFGWGLRPLSAQVLTADSVALVNLYDSTGGTNW
                     TTKTNWKTGPVSSWFGVTVGGNRVTAIDLNNNNLTGRIPVTLGTLGSLISLQLYGNAI
                     TNTISSSLGNLTNLANLGLENNQLSGQIPTSLGNLNAMTDLTLNNNAFTDSIPVTFRN
                     LAALQNLEVQNNRLSVFPDLSNIGTLTSLNLSGNKFTFEDIQPNVGIATFTYSGQDSL
                     DSYASVSSAEASSRILVVTAGGTNNQYSWKKNNVAIPSTNNDTLYIDSVKTSDEGNYT
                     CDVTNTVAIGTTLRRKVITLTVTGTAPSTPTGVTASGVTTNRIDVTWNKPSGVITRYR
                     VYRSTAAASGYAQVDSVAAPTLTYSNTSLSSNTVYFYKISSVGNFGFSAQSSAASDTT
                     LSVAPIVANAIPDTSLFEGFGKTFRRKLTHVFSDADNTTLTYSVSTTPAELTATISHD
                     TLYIASVAGFSGTAAVVASAF"
     gene            complement(1523..1978)
                     /locus_tag="PK937_11335"
     CDS             complement(1523..1978)
                     /locus_tag="PK937_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNH33208.1"
                     /translation="MPEDNEKIFARITTSPLSADSAYQFCLSPENGAVDIFIGTVRNE
                     FGGRPVEQIDYQAYPDMAEKILRTILQSACGQWPLNKVYAVHRIGLLKVTEASIVVAV
                     GAGHRGEAFEACRWIMEAIKRDLPVWKKEFFADGSVEWQHAGAAAISKK"
     gene            complement(1983..2231)
                     /gene="moaD"
                     /locus_tag="PK937_11340"
     CDS             complement(1983..2231)
                     /gene="moaD"
                     /locus_tag="PK937_11340"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNH33209.1"
                     /translation="MINVRVKFFAMCREAAGCEEAVIVLPASADAETFWQVIAEKFPR
                     LKAYQNQSRLAVNMEYVAGAVQFQNGDEVCIIPPVSGG"
     gene            complement(2237..7873)
                     /locus_tag="PK937_11345"
     CDS             complement(2237..7873)
                     /locus_tag="PK937_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNH33210.1"
                     /translation="MGSKVKKMIRLLLSISVVFLSSIHLNGCIAPDAMQATPMPETVF
                     GSGDFVLSATPQGTVADIQQFYAVVVIFAKPMVALSALPEGDGDGPLEISPSVNGKFR
                     WMGTNTLSFIPSEKLPLNTRFTVTIPAGTRALDGSAMSSPYVFSFETMRPKLISHNIS
                     EMNGTTLNPKIMLTFNQPIDIHSAKEKIRLIRIQDGANIPVSIEPADSADLLTYYNYG
                     YDQVAGSPVERIVRVKPITSLRMQTSYQLQIASGLKGREGVLGSSERVTHDISTYGPL
                     EFLELIPPEYPNSPFQLKFTNRIDADELKSHLTITPHTEINEIYPSWSSEYPASIYAQ
                     LQPQTDYTITIDPEIKDAFDNALGVTVRQNFRTGDYASSVYFETGEHVLESYLRHDLN
                     VAMINPGNIDIQMAAITQEEVIGLAKEGLYRDNGFNRWAHTISERPDLPRNATQYYPL
                     ALDPALASQPSGFVFVQMKHDTWNDYQRALIQVTDIGITAKFARDKSVIAVSQLKDGR
                     AARAFVQIRDDMGNLLWSGYTNSDGVAETQGWSGFGLQPIDTYNEPRLWVFATRGDQT
                     AYARSDNKIDLWRFNIPYAWYVQSAEDEISSKLFTNQGIYRPGDKVQFKAMLRRLQKG
                     AWSVPTGLNLKARIFNPSNDEVYAQDIVVNPFGGTDFEFTVPENAPLGYYYARLYTSE
                     KNKELSGESFQVQEFRPVESAVTINSVKSEYVWSETLKLNFDAHYLFGAPMANASMNW
                     SITRNASSFQPEGYDGYFFGKMYDYDGYYGRGAYQTVLASSSGTLDQHGLMDADVVLN
                     AGGVSETSELVIEGTVEDKNRQYVSGRTTVRVHQGAFYIGLKPSTTFMTWGEPLSVDV
                     VAVTPDGKKTADKKIELELIRREWHSVREKSYDGSYRWTSSQRDTTEEVFTVRSRGEI
                     VTQKLNPASTGYYILRATSSDPYDNTIISESYVYVTGESYAGWKLNDGDRIDLVANKS
                     QYKVGEKAKILVKSPYERARALVSVEREGIIRHFMTELKGNAASVEIPITAEMAPNAF
                     VSVTLLQGRTAEPVPDQNEDIGKPSFKIGYINLSVDPSANKLAVKIQTPKNKFQPGEW
                     MDVDVEVADKSGHGQIAEVTLYVEDVGVLNLVGYQTPDVFSHFYKQRELQVATTESRR
                     FILDQLVKRDLKEKGGVGGGGGEERSMPGIAMRKDFKSCVYWNPSVITDARGKARVRV
                     QLPDNLTMFRIFAVAHTRDSRFGSDDHSITVSKDLMLRPALPRFARIDDAVEAGAVLH
                     NNTTQAGTATIRAQAEGVEIIGAIEKTVELQPGASQEVRFQYRALKTGPAKFSFTAQM
                     RQFSDGVEITIPVRMPVPTETVALAGSTTDKTQEQVIIPGSIFENTGSFEIKTSSTAL
                     VDLDGSVKYLFEYPYGCLEQRTSRVLPVILFGDVVTAFGLTPFEDKTKSVEAAVKEYL
                     NEIPKFQQYGGGFSYWTGDHIVSPYVSVYAMFALTKAKAIGYDVPKEIMDNGVEYLKK
                     LVRNNPVDRYGVFYWHNTNAFALAVLADHDVYDAASAELLFQRRSELPLFARAMLLQA
                     IVKGGGHKAMTETLRRDLMNAIKMNPTTAHFEEPNLPGSEWSFHSPMRTTAAILQTFI
                     ELDGTNVPWAEKVVKYMLEERKQGRWRTTQENLYVFYALGTYFRNFEDVDPDFKATVR
                     LAGRQLLSQMYQGRTTQSHTSFTALAEFPSGKALLLDITKTGAGRLYYNARMTYAPRS
                     GGNAPARDEGLRVSKEIQDASGHVVTDGRYKAGKIYKITLTVSTAQARQYVVLDDPLP
                     AGFEAINTDLATSASSYGSGTENRWWEYGGFTRSEMRDDRVVAFADYFENGTQKFVYY
                     VRATTYGTFALPATKAEAMYHPEIFGTTAATQISVE"
     gene            complement(7884..8162)
                     /locus_tag="PK937_11350"
     CDS             complement(7884..8162)
                     /locus_tag="PK937_11350"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNH33211.1"
                     /translation="MDTMIVGLVSGILTTFGFLPQVIKSLRTKKVEDVAIAQPVVLLI
                     GMSGWLLYGVMRDDLVIIMANIVSIALNLWLIVLKIKYRSNVTANNGK"
     gene            8426..8734
                     /locus_tag="PK937_11355"
     CDS             8426..8734
                     /locus_tag="PK937_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33212.1"
                     /translation="MMRKMDAQFGIKLPPEFDESQETTRDISRWDAILSCAQSKGGRL
                     TAADVSIYFNVDLQTAEVRLDQCVESGHADLYVSQSGVKVYQFSHIISDDEKKRAEPV
                     "
     gene            complement(8783..10564)
                     /gene="aspS"
                     /locus_tag="PK937_11360"
     CDS             complement(8783..10564)
                     /gene="aspS"
                     /locus_tag="PK937_11360"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNH33213.1"
                     /translation="MSAIMNTAHRSTTCGELRAANAGQKVTLIGWIHRRRDLGQVIFI
                     DLRDRYGLTQIVFNPDKFPETHEKAKELRSEFVIKVLGVVTPRESVNKNIPTGEIEVI
                     TESLTIMSRAKTPPFEINDENLKVDEDLRLEYRYLDLRRSYMQETLVLRHRLAQIVRN
                     YFTENQFVEVETPVLMKSTPEGARDYLVPSRVHAGKFYALPQSPQIYKQLLMVSGMDR
                     YFQIVKCFRDEDLRADRQPEFTQVDVEMSFVEREDILGMIERLMFKIFKELKDIDLTM
                     PLRRMPYFEAMRMYGSDKPDLRFGMPITYIEECVRGSEFKVFNDVLDQKDGAIALLCV
                     ESQASSFSRKKIDELTEFVKKHGLLGLATVKVEATGITSSIGKFFTEDKLRRMAEVAG
                     AKEGDLLLIAAGKEQIVQSALGALRLKLAEDLKLVRDDAYAMFWVVDFPLFEYDEEEK
                     RWNAMHHPFTSPFDEDLSKMDSEPGAVRAKAYDLVLNGSEVGGGSIRIHSRDVQNKMF
                     DALGLTQEDRDTKFGFLLRAFEYGVPPHGGIALGFDRLTAILAARKSIRDVIAFPKTN
                     SAVSLMDQSPSPVDARQLKELHIRTTE"
     gene            complement(10659..11726)
                     /locus_tag="PK937_11365"
     CDS             complement(10659..11726)
                     /locus_tag="PK937_11365"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNH33214.1"
                     /translation="MNSVKRNTKAAAAPPGGARAAGAARDIRIVAFPKEFQKSYWSAF
                     DKRMLGIFFASLVIVYAPLIYMAMQPPRPKPTVLDNKLLKAIAKINKVDAALLEELDK
                     PKEEEKTEETATSSVPSAQINRQQAPTNAQGRATRAAAAKAAAAARAGAAAKAAAGRG
                     VLAVAGAVGAGGGGRYAAVDFSGGSGNLDEVLGQVGGIGEATGGGGDRTVLGAGGVAG
                     GTGSISDLTAELGGAGALSVAAGSAGSGLIGVGKASISGTGGGQGASAAEIQGVIDQN
                     ATSVNACYQKELKKSPDLKGKLSVSIKINQAGRVSGVSVTQNTVGDAVANCVTNKIRG
                     WTFPRGKKGVVTVNQTFVFTK"
     gene            complement(11771..12277)
                     /locus_tag="PK937_11370"
     CDS             complement(11771..12277)
                     /locus_tag="PK937_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH33215.1"
                     /translation="MAFKPSAAKKNKRSEEGTLNMNSMMDILTIMLLFLLMNFSTSGA
                     LATKSDGMQPPKIVTKNKPKKMLLINVSQAHIFYNKDAIAETETVLAQKNSFLIPQLA
                     EKLEEDANKALDLEARFGIEFKREVIVVCDDRTPFNVLMKVVVTCGRNQFSNLRLLGA
                     LANKNDVI"
     gene            complement(12320..12895)
                     /locus_tag="PK937_11375"
     CDS             complement(12320..12895)
                     /locus_tag="PK937_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH33216.1"
                     /translation="MAFKPSQRRSVKAESTELDLRPIMNMMCILIPLLLKCSEFVKMT
                     YLELNLPPLSGGGGGGGADDKPEKEKPKIGLKLVVTEKGMTIAGNSIVLAGEGGTTGP
                     TLPKLADGKYDFRGLEQKMKDIVKQINGKGFEDERTIIITAEDVIPYQAIVTAMDIIT
                     NSEFKEFSDPSSNQKVKQPWFANIGVGKIII"
     gene            complement(12980..13765)
                     /locus_tag="PK937_11380"
     CDS             complement(12980..13765)
                     /locus_tag="PK937_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNH33217.1"
                     /translation="MKKNILAKAAGLTVTAQMMASSLFAQDGGAAAPQESQSQVAIFF
                     DAFFQSFKWEEAGSTYMWIIAFCGVFSVAIMAERLYFIFVRSNVDANKFMAEVRKLVA
                     GGNLKKAIALCETAKDKALPSVVLAGLKRAAEKEGGPLDFRAIQNAVDEGTLEIIPKL
                     SERSGYLAMLSNVSTLFGLMGTVYGLILAFAAVGNPAMPEDQKALFLAKGISAAMQTT
                     IWGLSVAIPCIIVYTLINTKTGKIIDEMDEHMVKLINLMTGNK"
     gene            complement(13895..14377)
                     /locus_tag="PK937_11385"
     CDS             complement(13895..14377)
                     /locus_tag="PK937_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33218.1"
                     /translation="MNKLMCWLCILGAMLVFSVAASRIHAQDVKHPDMEVIENDAGEQ
                     IVVVLGTNNPPDAKLYTIKEEEGAKGPILVVYYKPTGDMVQFFSKTTIRGKSEGPGVS
                     LFANRVSPQIEKVIGQKSYERELKKRYLDLTAIDKSTIVGSERVNLEEIDKMIKKRRN
                     "
     gene            complement(14387..>14847)
                     /locus_tag="PK937_11390"
     CDS             complement(14387..>14847)
                     /locus_tag="PK937_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33219.1"
                     /translation="FTMQDTNAKPIYSQDQINKIMDTAKVAYFRTSFNVKGSPLSGKL
                     YIAVDGYYEFYLNGAFVGTALQESGEADSIGNVLETTELFIENFAQGRNVLGIMMHDR
                     ESPKEHHGIRIVLEVTEIEDPTAAIRDAAPPEGQELKNTLFRRGRVAKGK"
BASE COUNT         3835 a         3756 c         3275 g         3981 t
ORIGIN      
        1 aaatgcgctt gcaacgacgg ccgctgtacc gctgaacccg gccacactgg caatataaag
       61 tgtatcgtga gaaatcgttg cggtaagttc cgcaggcgtc gtggagaccg aatacgtcaa
      121 tgttgtatta tccgcatcgg aaaaaacatg cgtcagtttc cggcgaaacg tctttccgaa
      181 cccttcaaaa agcgaggtat cgggaatggc attcgccacg atcggcgcta cgctaagcgt
      241 tgtatcactt gcagccgacg attgcgcgct aaaaccaaaa ttacctacag acgagatttt
      301 ataaaaataa acggtattgg acgaaagact ggtattgctg taagtcagtg tgggcgccgc
      361 aacggaatct acctgtgcat aaccggaagc cgctgccgtc gaacgataca cgcggtagcg
      421 tgtgatgaca cccgaaggtt tattccaagt cacatcaatg cggttggtcg tcacaccgga
      481 ggccgtaacc cctgtcggtg tggatggcgc agtaccggtc acggttagtg ttattacttt
      541 tcggcgcagt gtagtcccta tcgcaactgt attggtcaca tcgcacgtat agtttccctc
      601 atcgctggtt ttcacggaat caatgtacaa cgtgtcatta ttggttgaag gtatagcgac
      661 attgtttttc ttccatgaat attgattatt tgtgccacct gcagtgacta ctaaaatacg
      721 cgaagacgcc tccgctgagc tgaccgatgc ataactatcc aaactgtcct ggccggagta
      781 tgtaaatgtg gcgataccga cattgggttg aatatcttca aaagtaaatt tattacccga
      841 aagattgagc gaagtcaaag tgccgatatt tgaaagatca ggaaatacgc tcagacgatt
      901 gttttgtact tccagatttt gcagggcggc aagatttcta aaggttacgg gtatagaatc
      961 cgtaaacgca ttgttattca gtgtcagatc cgtcatggcg ttaaggttgc ccagtgaagt
     1021 cgggatttgg cccgaaagct gattattctc aagaccaaga ttggccaaat tggtcaggtt
     1081 gcccaagctg gaagaaatag tattcgtaat ggcattgcca tacaactgaa gtgaaatgag
     1141 gcttcccaaa gtaccgagtg taaccgggat acgtccggtc agattattgt tattcagatc
     1201 aatcgccgtt acacgattgc caccgacggt tacgccaaac caagagctca ccggtccggt
     1261 tttccaattg gttttagtcg tccagttagt gccgccggta ctgtcataga gattgaccag
     1321 cgccacggag tcggctgtga gcacctgcgc cgatagcggt cgcaaacccc acccgaagat
     1381 aaatgtaaaa acaacgacga aacatgattt cataaaagcc tcagtgcatg ttcgcaaaca
     1441 aatattaatt cgatgtaaat tatcagattt gattgacggc aagatcatta aaaattagtt
     1501 aaaaaacaat agcctagcaa gtctattttt tagaaatcgc ggcggctccg gcatgctgcc
     1561 actccacgct gccgtcggca aaaaattctt tcttccagac cggcaaatct cgttttattg
     1621 cttccataat ccagcggcat gcttcaaatg cttcgccccg atgccccgca ccgacggcga
     1681 ccacaatact ggcttcagta actttcagca aaccgatccg gtgtaccgca taaaccttat
     1741 taagcggcca ttgcccgcac gccgactgaa gaatcgtgcg cagtattttt tccgccatat
     1801 caggataggc ctgataatct atttgctcta ccggtctgcc gccgaattcg ttgcgcaccg
     1861 taccgataaa aatatccaca gcaccgtttt ccgggctgag gcaaaattga taagccgaat
     1921 ccgccgaaag cggcgatgtc gtgatccggg caaaaatttt ttcgttatct tcaggcataa
     1981 ctttatcctc cgctgaccgg cggtataata cacacttcat caccgttttg aaattggact
     2041 gcgccggcaa cgtattccat atttacagcc aggcggcttt ggttttgata tgccttcaag
     2101 cgcggaaatt tttcggcgat aacttgccaa aaagtttctg catccgcgga agccggtaat
     2161 acaattacag cttcctcgca tccggccgct tcacggcaca tcgcaaaaaa tttaacgcgt
     2221 acattgatca tgttcatcat tccactgaaa tctgagttgc cgccgtcgtt ccgaaaatct
     2281 ccggatgata catcgcttcg gctttggtag ccggcagcgc aaacgtaccg taggtcgtcg
     2341 cacgcacgta atagacaaac ttctgtgtcc cgttttcaaa atagtcggca aaagccacga
     2401 cgcgatcatc gcgcatttcc gaacgcgtaa atccgccgta ctcccaccaa cgattttctg
     2461 tcccactgcc gtacgaagat gccgaagttg ccaaatccgt gttgatcgct tcaaatcccg
     2521 ccggcaacgg atcatccaac accacatatt ggcgtgcttg cgccgtactg accgtcaatg
     2581 tgattttata aattttaccg gctttataac gtccgtccgt gacgacgtga cccgaagcgt
     2641 cttgtatttc tttgctcacc cgcaatccct catcgcgtgc cggagcatta ccgcctgaac
     2701 gcggcgcata agtcatacgc gcattgtaat ataaacgccc cgcgcctgtt ttggttatat
     2761 caagcagcaa tgctttaccg ctcggaaatt cggctaaagc cgtaaaggat gtgtgactct
     2821 gtgtcgtccg gccctgatac atttgagaga gtaattgacg ccctgccaaa cgcaccgttg
     2881 ctttgaaatc cggatccacg tcttcaaaat ttctgaaata cgtacccagc gcgtaaaaca
     2941 catacaagtt ttcctgcgtc gtacgccagc gtccttgttt acgttcttca agcatatatt
     3001 tcactacttt ttcagcccaa ggtacgttgg tgccgtccag ttcgataaac gtttgcaata
     3061 tcgccgcggt ggtgcgcatc ggcgaatgga atgaccattc cgaaccgggc aaattcggct
     3121 cttcaaaatg cgccgtcgtg ggattcattt taatcgcatt catcaaatca cgacgcaatg
     3181 tttcggtcat ggctttatga ccgccgcctt tgacaatcgc ttgcaaaagc attgcgcgtg
     3241 caaacaaagg taattcggaa cggcgctgga ataacagttc ggcgctggct gcatcataca
     3301 catcgtgatc ggccaataca gccaatgcaa acgcattggt attgtgccaa taaaaaacac
     3361 catagcggtc taccggatta ttacgaacaa gctttttcaa atattctacg ccgttgtcca
     3421 tgatttcttt gggtacatca taaccgatag ctttcgcctt ggtcaaagca aacatcgcat
     3481 acacgctgac atagggcgat acgatatggt cgccggtcca atacgaaaaa ccgccgccat
     3541 attgctgaaa tttgggaatc tcattcaagt attctttaac cgccgcttcg accgattttg
     3601 ttttgtcttc aaacggagtg agaccaaatg ccgtcaccac gtcgccaaaa agaatgaccg
     3661 gtaaaacacg ggaggtgcgt tgttcgaggc agccgtaggg gtattcgaaa agatatttta
     3721 cactaccgtc aaggtcaacc aatgccgtag acgaagtttt gatttcaaaa cttcccgtgt
     3781 tttcaaaaat cgaaccagga atgatcactt gttcctgcgt tttgtccgtc gtactgccgg
     3841 caagcgccac ggtttccgtt ggtacgggca tacgcaccgg tattgttatt tctacgccat
     3901 cgctaaactg ccgcatctgt gcagtaaaac taaatttcgc cggcccggtc ttaagcgcac
     3961 ggtattggaa acgcacttct tgggatgcgc cgggttgcag ctccaccgtt ttttcgatgg
     4021 caccgatgat ttcgacgcct tccgcctgtg cgcgtatcgt cgctgtcccc gcttgcgtgg
     4081 tgttattgtg tagcaccgcg ccggcttcca cggcatcatc tatgcgggca aaacgcggta
     4141 gtgcaggccg aagcatgaga tctttgctga ccgtaatgga atgatcgtca ctgccaaaac
     4201 gagaatcgcg ggtatgggcc accgcaaata tgcgaaacat agtaaggttg tccggcagtt
     4261 gtacacgtac tcgcgcctta ccgcgcgcat cggtgatcac ggaaggattc cagtacacac
     4321 aggatttgaa atctttgcgc atggcaatgc ccggcatact tcgctcttca ccgccgccac
     4381 cgccgacacc gcccttttct ttaagatcgc gctttaccag ttgatccaaa ataaatcgcc
     4441 gcgattcggt cgtagcaacc tgcaactcac gttgtttgta aaaatgtgaa aacacatccg
     4501 gcgtttgata tcccaccaga ttgagtacgc cgacatcttc gacataaagc gtcacttccg
     4561 caatttgacc atgccctgac ttgtccgcca cttcgacgtc cacatccatc cattcaccgg
     4621 gttgaaattt atttttcggt gtttgaattt tcacggccag tttgttagcc gacggatcca
     4681 cagaaaggtt gatatagccg attttaaaag acggctttcc tatgtcttca ttttgatcgg
     4741 gcacaggttc agcggttcgc ccttgtaaaa gcgtcaccga cacaaacgca ttgggcgcca
     4801 tctcggccgt gatcggaatt tcgaccgaag cggcattacc tttgagttcc gtcatgaaat
     4861 gacgaataat accttcgcgt tctacactca ccagagcacg cgcgcgctca taaggcgact
     4921 tgacgagtat tttggctttt tcaccgactt tatattggct tttattcgca accaaatcaa
     4981 tccggtcgcc atcattgagt ttccatccgg cataggattc gccggtcaca taaacgtagg
     5041 attctgaaat gatcgtattg tcgtatggat cgctcgaagt cgctctcaag atataataac
     5101 ccgtcgaggc cggattcaat ttttgcgtga cgatctcccc gcgcgagcgt actgtaaaaa
     5161 cctcttccgt tgtatcgcgc tgagaagacg tccaccggta cgaaccgtca tacgattttt
     5221 cccgaacgga atgccattca cggcgaatca attccaattc aattttttta tcggccgttt
     5281 tttttccatc aggcgttacg gctacgacat ccaccgacaa gggttcaccc catgtcataa
     5341 acgtcgttga tggtttgagg cctatataaa atgcgccttg atgcacgcgc accgttgtcc
     5401 ggccgctgac atactgcctg tttttatctt ctaccgtacc ttcgatgacc agttccgaag
     5461 tttcggaaac accgccggca ttaagcacaa cgtcggcatc cataaggcca tgttgatcca
     5521 atgtcccgga ggagctcgca agcaccgttt gatacgcacc acgcccgtaa tacccgtcgt
     5581 aatcgtacat tttaccaaag aaatacccgt catacccttc cggttggaat gagctggcat
     5641 tacgcgtaat actccagttc atggatgcat tggccatagg cgcaccaaaa agatagtgcg
     5701 catcaaaatt aagtttgagc gtctcactcc atacgtactc ggatttaacc gaattgatgg
     5761 tcaccgcact ttcgacagga cgaaactcct gcacctggaa cgattcgccg gagagttctt
     5821 tatttttttc cgacgtatac aaacgcgcat aataataacc gagcggcgcg ttttcaggaa
     5881 cggtaaattc gaaatccgtt cctccaaacg gattgactac gatgtcttgg gcatacactt
     5941 catcgttgga cggattaaaa atacgagctt tcagattgag tcccgtcggt acggaccacg
     6001 cgcctttttg taagcgacgc agcattgctt taaattgcac tttatcgccc ggcctgtaga
     6061 tgccttgatt ggtaaataac ttagacgaaa tttcgtcttc ggccgactga acataccacg
     6121 catatggaat attaaagcgc cataaatcaa ttttattatc cgagcgcgcg taggctgttt
     6181 gatcaccacg cgttgcaaat acccacagcc tcggttcgtt gtatgtatct atcggttgga
     6241 gaccaaaacc gctccagccc tgtgtttccg ctacgccatc cgaattggta tagccactcc
     6301 acaacagatt acccatatcg tcgcgtatct gcacaaaggc acgtgcagca cggccgtctt
     6361 tgagctggct gacggcaata acgcttttat cgcgggcaaa ttttgccgta atgccgatat
     6421 ccgtgacttg tatcagtgcg cgttgatagt cattccatgt atcatgcttc atctgtacga
     6481 agacgaaacc cgacggttgg gaggccaacg ccggatccaa tgcgagcgga taatattgtg
     6541 tcgcattacg cggcagatca ggacgttcag atatcgtatg tgcccaacga ttaaaaccgt
     6601 tatcgcgata taaaccctct ttggctaaac caatgacttc ctcttgggtg atagcggcca
     6661 tttgaatatc aatattgccc ggattgatca tcgccacatt cagatcgtgg cgcagatacg
     6721 attctaaaac atgttctccg gtttcaaaat aaaccgacga cgcgtaatcg cccgttctga
     6781 aattctgccg aaccgtaact cccaaagcat tatcaaaagc atcttttatc tcgggatcaa
     6841 tggtaatcgt ataatccgtt tgcggctgaa gttgcgcata gatactcgca ggatactccg
     6901 acgaccacga tggatatatt tcattgatct cggtatgcgg tgtgatcgta agatgcgatt
     6961 tgagttcatc ggcatctatg cgattggtga actttagttg aaaaggacta ttgggatatt
     7021 ccggcggaat caattccaaa aactccaaag gcccgtaggt cgaaatgtca tgcgtcacgc
     7081 gctcggatga acccagtaca ccttcgcgtc ctttgaggcc ggatgcgatc tgcaattgat
     7141 aactcgtctg catgcgcaac gatgtgatcg gttttacgcg cacaatgcgc tcaacgggtg
     7201 atccggcaac ctggtcataa ccgtagttgt aatacgtcag taagtccgcg ctatcggccg
     7261 gttcgatgct aaccggtata ttggcgccgt cctgaatacg aatgaggcgt atcttttcct
     7321 tggcggaatg gatatctatc ggttgattaa atgtcagcat gattttcgga ttgagcgtag
     7381 tgccattcat ctccgaaata ttatgactga tcaatttcgg tcgcatggtt tcaaacgaaa
     7441 aaacatacgg cgaagacatc gcgctgccat cgagagcacg cgtgcctgcc ggtatggtta
     7501 cggtaaatcg tgtattcagt gggagttttt cggacgggat aaaagacaat gtattcgttc
     7561 ccatccagcg gaatttgccg tttactgaag gacttatttc cagcggcccg tcaccgtcgc
     7621 cttcgggcaa agcgctcaac gccaccatcg gttttgcgaa aatcacaaca acggcataaa
     7681 actgctgaat atctgcgacc gttccttgcg gtgttgccga caatacaaag tccccggaac
     7741 cgaaaaccgt ttcaggcata ggtgtggctt gcatggcgtc cggtgcaata caaccattga
     7801 ggtgtatcga agataaaaaa accaccgata tcgataacaa cagacgtatc atttttttca
     7861 ctttagagcc catgaccatc tccctatttc ccattatttg ctgttacatt ggaacgatat
     7921 tttattttaa gcacaatcaa ccaaagattc aatgcgatgg aaacgatatt agccataatg
     7981 ataactaagt catcacgcat taccccatag agcaaccatc cgctcatccc gatcaataaa
     8041 acgaccggct gagcaatagc cacatcttca acttttttag tacgaagact tttgatcact
     8101 tgcggtagaa aaccaaacgt agttagaatt ccagaaacca aacctacgat cattgtatcc
     8161 atgaagacca ttcaatcatg ttttgagttg agtatttttt atatacatta catttcaata
     8221 acttatctga ataaggataa atcttttatg aaagacattt caggggcgat taataacctg
     8281 ctgaacaaaa ttcaaaattt gggtgctgct tggcggtgtt gtaatcataa cgatcgcttt
     8341 tacggccccc caattcctgt ttattttgat attccctcta ttcttcggat tatttttcta
     8401 catattcaaa agcttcgtac aacccatgat gcgcaagatg gatgctcaat ttggcatcaa
     8461 gcttcctcct gaattcgatg agagccaaga aacaactcgc gacatttcgc gttgggatgc
     8521 aatactatcg tgcgctcaat ccaaaggcgg ccgtctgacc gcggccgatg tttcgattta
     8581 ttttaatgtg gatctgcaga cggcggaggt acgtcttgat caatgcgtgg aatcaggaca
     8641 tgcggatcta tatgtaagcc agtccggcgt taaagtatat caattctctc acattatcag
     8701 cgacgacgaa aaaaaacgcg ctgagcctgt ataacttttt taaagcgcat ggttttatgc
     8761 tcatgcgctt taacatatcc gattattccg ttgttcgtat gtgcagttct ttcagttgtc
     8821 gtgcatctac gggcgaaggc gattgatcca tcagtgaaac ggcgctattg gttttaggaa
     8881 atgcgatcac atcgcggatg gatttccgtg cggccagaat cgccgtcaaa cgatcgaatc
     8941 ccaatgcgat accgccatgc gggggtacgc catattcaaa tgcgcgtagt aaaaatccga
     9001 atttggtatc gcgatcttcc tgtgtaagcc ccaacgcgtc aaacatttta ttttgtacat
     9061 cgcgactgtg aatacggata ctcccgccgc cgacttccga accgttgagt accagatcat
     9121 acgcttttgc gcgcacggcg ccgggttccg aatccatctt cgaaagatct tcatcaaacg
     9181 gcgacgtaaa cgggtggtgc atcgcattcc agcgtttttc ttcttcatcg tactcgaaaa
     9241 gcggaaagtc aacaacccaa aacatcgcat atgcatcgtc acgtacgagt ttcaaatcct
     9301 cggcgagttt caaacgcagc gcgcccaatg cggactgtac gatttgttct ttgcctgcgg
     9361 cgatcaaaag caaatcgccc tctttagcgc cggccacttc ggccatccgg cgcaatttat
     9421 cttcggtgaa aaatttaccg atagatgaag ttatgcccgt cgcttcaact ttgaccgtcg
     9481 ctaaacccag gaggccgtgt ttttttacaa attccgtcaa ctcatctatt tttttgcgcg
     9541 aaaaagagga ggcttgcgat tccacacaaa gtagagcgat cgcgccgtct ttttgatcaa
     9601 gtacatcgtt gaaaaccttg aactccgaac cgcgcacaca ttcttcgata tacgtgatgg
     9661 gcataccaaa acgcagatcc ggcttatcgc tgccgtacat tcgcatcgct tcaaaatacg
     9721 gcatacgacg caacggcatc gtaaggtcta tgtctttgag ttccttaaaa attttgaaca
     9781 tcagacgttc aatcatgccc agtatgtctt cgcgttcgac gaacgacatt tccacgtcca
     9841 cctgcgtaaa ttctggctga cggtcggcgc gtaagtcttc atcacggaaa cacttgacga
     9901 tttgaaaata gcgatccatg cccgacacca tcagaagctg tttgtaaatc tgcggtgact
     9961 gcggcaaggc ataaaatttt ccggcgtgga cgcggctggg cacaagataa tcccgcgcgc
    10021 cttccggcgt gcttttcatc agtacgggtg tctcgacttc gacgaattga ttttcggtaa
    10081 aataattgcg tacgatttgc gccagacgat gcctcagtac aagtgtttcc tgcatatatg
    10141 aacgccgcaa atcaaggtaa cggtactcca atcgcagatc ttcatctact ttgaggtttt
    10201 catcattgat ttcaaatggc ggtgttttgg cacggctcat gatagtcagc gattccgtga
    10261 tcacttcgat ttcaccggta ggaatattct tattcaccga ttcccgaggt gtgacgacgc
    10321 ctaaaacttt gataacaaac tcactgcgaa gctcttttgc tttttcgtgg gtttcaggga
    10381 atttatccgg attaaaaacg atctgggtta aaccataacg atcacgcagg tctataaaaa
    10441 tgacctgtcc caaatcgcga cggcggtgaa tccatccgat caacgttact ttttgcccgg
    10501 catttgcggc acgcagttca ccgcatgtcg tactgcggtg agccgtattc ataatagcgc
    10561 tcatactttc tctgatttcc gatctatctt gataattaaa tataaaaaaa accgccacgc
    10621 ttggggcgtg acggttttat cacaatgtac gataatgctt acttcgtgaa gacgaacgtc
    10681 tggttaaccg ttacgacgcc ttttttaccg cgcgggaaag tccacccgcg gattttattg
    10741 gttacgcaat tagcgacggc atcgccaacg gtattttgcg taacactcac accggaaacg
    10801 cgacccgctt gattgatttt aatcgaaacg gataacttac ctttaagatc cggtgatttt
    10861 ttgagttctt tttggtaaca cgcattgacg gaggttgcgt tttggtcaat aacgccttgg
    10921 atttctgccg ctgacgcacc ttgtccgcca ccggttcctg agatcgaagc tttaccgaca
    10981 ccgataagac cgctaccggc gctaccggcc gctacagaca atgcgcctgc accgccaagc
    11041 tcagccgtga ggtccgaaat gcttccggtt ccgccggcta caccgcccgc gccaagcacg
    11101 gtacgatcac cgccaccacc ggtcgcttca ccgatacccc cgacttgacc caacacttca
    11161 tcaagattac cgctgccgcc ggagaaatca acagccgcat aacgaccgcc accaccggcg
    11221 ccaaccgcac ccgcaacagc caaaacacct cgaccagcag ccgcttttgc ggccgcaccg
    11281 gcacgtgctg ctgcagcggc tttagccgcg gcggcgcgcg ttgcacgacc ctgagcattc
    11341 gtgggcgctt gctgacgatt gatctgcgca ctgggaacag atgatgtagc tgtttcttca
    11401 gttttttctt cttcttttgg cttatccaac tcttcaagaa gcgcggcgtc aactttgttg
    11461 attttcgcaa tagctttcaa aagcttatta tcaagcacag tcggtttggg acgcggtggt
    11521 tgcatcgcca tatagatcaa cggcgcatat acgatcacca aagaggcaaa gaaaataccg
    11581 agcatgcgtt tatcaaacgc agaccagtaa cttttctgaa actctttagg aaacgcaacg
    11641 attcgaatat cgcgtgccgc gccagctgcg cgtgcaccgc ccggaggggc tgccgcagct
    11701 ttagtatttc gttttactga attcattccg gactccttct cgagtcaatt cacaaaatta
    11761 aattgtttta ttagatcaca tcatttttgt tggccaatgc gcctaacaaa cgaagattgg
    11821 aaaactgatt acgaccgcaa gttacaacga ctttcatcag tacgttgaaa ggtgtacgat
    11881 catcgcaaac cacaatcacc tcacgcttaa attcaatacc aaatcgcgct tccaaatcca
    11941 aggctttatt ggcatcttct tcaagctttt cagcaagctg tggaatcaaa aatgaattct
    12001 tttgcgccag taccgtttcc gtttcggcta ttgcatcttt gttgtaaaaa atgtgtgcct
    12061 gagaaacgtt gatgagcaac atcttcttag gtttgttttt ggtaacgatt ttcggcggtt
    12121 gcataccgtc cgacttcgta gccaaagcgc cggaagtgga aaagttcata agaaggaaga
    12181 gcaacatgat agtcagaatg tccatcatgg agttcatatt cagcgtacct tcttcgctac
    12241 gcttattctt ttttgctgca gatggtttaa atgccatgta aaatattcct ttaggaaact
    12301 aaatttcggc gcctatggtt tagataatga tcttaccgac accgatattg gcaaaccacg
    12361 gttgttttac tttttgatta ctgctcggat ccgaaaactc tttgaattcg gagttggtga
    12421 tgatatccat cgcggtgacg atggcctgat acggaattac atcctctgcc gtaatgataa
    12481 tggttcgctc atcttcaaaa cccttaccgt tgatttgttt gacgatatcc ttcattttct
    12541 gttcaagacc tctgaagtcg tatttaccgt ccgccaactt aggaagcgtc ggacccgtag
    12601 taccgccttc accagccaaa acgatcgaat ttccggcaat cgtcatacct ttttcggtaa
    12661 caactagctt aagcccgatt ttcggttttt ctttttcagg cttatcatcc gcgccgccac
    12721 cacctccacc accgctcagc gggggaaggt ttagctccaa ataagtcatt ttaacgaatt
    12781 cggaacattt gagaagcaac ggaatcaaga tacacatcat gttcatgatg ggccgcagat
    12841 caagctctgt gctctcggcc ttaacactcc gtctttgaga gggtttaaat gccattgtaa
    12901 aaccctttta aattataaaa gttctaataa atcttaggtg ttgaaatcaa agcgataatc
    12961 actcgctaag atactgtaat tatttattac ccgtcatcaa attgatgagt ttgaccatgt
    13021 gttcatccat ttcatcaatg atcttaccgg tcttggtatt gatcaatgtg taaacgatga
    13081 tacagggaat agcaacagaa agaccccaga tcgtcgtttg catagcggca gagatacctt
    13141 tcgcgaggaa caacgctttt tgatcttcag gcatggccgg gttacctacg gcggcgaaag
    13201 cgagaatcag accgtacacc gtacccatca gaccgaaaag ggtcgaaacg ttggataaca
    13261 tagcgaggta accggagcgt tccgagagtt ttggaatgat ttcgagtgta ccttcgtcaa
    13321 cggcattttg aatggcacga aaatctaacg gaccgccttc tttttcagcc gcacgtttca
    13381 gaccggcaag cacaaccgac gggagcgctt tatcttttgc tgtttcgcaa agcgcaatcg
    13441 cttttttgag gtttccaccg gcaaccagct tacgaacttc agccatgaat ttgttggcat
    13501 ctacattgga acgcacaaaa ataaaataaa gacgttccgc catgatggcc acggaaaaaa
    13561 caccgcaaaa cgcaatgatc cacatgtaag tagaacctgc ttcttcccat ttgaatgact
    13621 ggaaaaatgc atcaaagaaa atggctacct gtgattgcga ttcttgagga gccgcagcgc
    13681 cgccgtcttg ggcgaaaagc gaagaagcca tcatttgggc tgtcactgtc aagcctgctg
    13741 ccttagccaa gatgttcttt ttcatgaata ctaacctccg gttatgtttc agggtacttt
    13801 aatgaggaat gtataaaaaa tttcgtgatt cagacttaga tacgaacggc atacaattta
    13861 aatgcttttg attgaaatgc aagaaaatta aaaatcaatt acgccgcttt ttgatcattt
    13921 tatctatttc ttccagattg acacgttcgg aaccgacgat cgtactttta tcaatcgcag
    13981 tcaaatccaa atatcgtttt ttaagttcac gttcgtacga tttttgtccg atcacttttt
    14041 cgatttgcgg tgaaacacga ttagcaaaaa gcgaaacacc gggaccttcc gatttcccgc
    14101 gaatggtcgt cttgctgaaa aactgcacca tgtcccccgt gggcttataa tacactacaa
    14161 gaatcggccc cttggcgcct tcctcttctt tgatcgtata gagtttggca tccggaggat
    14221 tattcgtacc aagcacgact acgatttgtt caccggcatc gttttcgatt acttccatat
    14281 ccgggtgttt gacatcctgt gcatggatac gcgatgcggc tacgctaaac accaacatcg
    14341 ctccgaggat gcacaaccaa cacattaatt tattcatacc cactccttac tttccttttg
    14401 cgacacgtcc gcgacggaag agcgtgtttt taagttcctg tccttccgga ggcgcagcat
    14461 cccgaatcgc cgccgtagga tcttcgattt cggttacttc gagaacgata cggataccat
    14521 gatgttcttt aggtgattcg cggtcgtgca tcatgatacc taatacgttg cgaccttgag
    14581 caaagttttc gataaacagt tctgttgttt caagtacatt accgatacta tccgcttcac
    14641 cggactcttg caatgccgta cccacaaatg caccgttcag ataaaactcg taatatccgt
    14701 ccacggcgat gtaaagttta cccgacaacg ggcttccttt gacattaaaa gaagtgcgga
    14761 agtatgccac tttggccgta tccatgatct tattgatctg gtcttgcgaa tagatcggct
    14821 ttgcattcgt atcctgcatc gtaaaat
//
LOCUS       DAOQGF010000085        14729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1871_length_14729_cov_6.260801, whole genome shotgun sequence.
ACCESSION   DAOQGF010000085 DAOQGF010000000
VERSION     DAOQGF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_14729_cov_6.260801"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..830)
                     /gene="amrB"
                     /locus_tag="PK937_11395"
     CDS             complement(15..830)
                     /gene="amrB"
                     /locus_tag="PK937_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNH33220.1"
                     /translation="MRRPAVAGLFYQADPAKLRDEVSGLLDNTTTQFSGGQPRGLIVP
                     HAGYRYSGYVAAEAFATIRGMSYHTVLILSPSHHDYFHGISVIRESYRTPLGDVPVAL
                     EAVDRILDRCALCKINILGHRDEHGIEVELPFLQVVLKNEFSIIPLVFGTQDNETIHA
                     LSEALTPFAGDPGVLLVASSDLSHFYNAVEGEKKDSIFLENVTAMDDDALYTNIKTRK
                     TEACGYGPVLAVMRAMKKNGITDSRILMYRHSGHVSGDLEEVVGYAAGLFYKH"
     gene            complement(866..2296)
                     /locus_tag="PK937_11400"
     CDS             complement(866..2296)
                     /locus_tag="PK937_11400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH33221.1"
                     /translation="MSDSAVEKKKILVVDDDKNILRLIQAYLENDEYRIYNATDGKEC
                     FAMVDQDIPDVILLDLRIPGMDGIEVMKSLRIYYPKIPIIMMSAHGTIQSAVDAMQTG
                     AYDFVTKPFDSNRLKVSVRNALERKTKDDEIERLQKELYGPRDYGSIVGSSPAMRRIY
                     DLMDKIVASSNVTVLITGESGTGKELVAREIHKHDKSREKKPFVAFNCAAIPEHLLES
                     ELFGHEKGAFTGATERRIGKFEFAHGGTLFLDEIGEMSMATQAKLVRVIQEREVSRIG
                     GNDVIKIDVRLISATNKNLEEEVKNGRFREDLFYRLSVFPIVMPSLRERKEDVALLAT
                     HFMEKFKKREKKENISGMSREVLELLMGYHWPGNVRELENSMERAMVLASGNAITLED
                     LPPNIRTLSAPKPGYGKEMYMDMTGTMEEILERVEEKIIKKCFADCDGNISEVARRLN
                     IGRATIYRKAEKYGLPIKDYSTGESS"
     gene            complement(2307..3392)
                     /gene="lpxK"
                     /locus_tag="PK937_11405"
     CDS             complement(2307..3392)
                     /gene="lpxK"
                     /locus_tag="PK937_11405"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNH33222.1"
                     /translation="MIIKPPVQWKNSLLRYITYILFFPLSLLFAFLLLIKNSIAKLRP
                     VKNKVRVICIGSLSVGGSGKTPFVEWYARLLVARGLKLCVISNGYRKKSHGLVCVSDG
                     IKMNASLDASGDEAYMMAINFLNDNLNIPVISSPNRSHGIDFAEKNYDPDIILLDDGF
                     QDFSVYKDAVLCVEDYWERDYPQLWLPLGRLRDLKQNIKKCNAHVVTKIPPHAVLQTE
                     AGALPFLGFTYYRYAMYDLDDHPLNFEILKHKKIIVLAGLGHNDAFLRTVESIARDNS
                     AEVIHFAEFHDHHWYSRADIRRILSLQGSDTNDYIVLTSQKDAIKVKKEWVPSGFRFF
                     YLKPFMKPVHDAAADELAAIALRLKRQ"
     gene            complement(3382..3858)
                     /locus_tag="PK937_11410"
     CDS             complement(3382..3858)
                     /locus_tag="PK937_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HNH33223.1"
                     /translation="MILFWHAHIFSVIAWLVVHNKSDQCVALVSASEDGQKLACVLKR
                     LGFTVVEGSSSRYGFEALLALRSLKYKNHWILITPDGPKGPARTCKPGFASLAQMHTR
                     YIHAVSLHTTNAWNLNSWDRAQIPKPFSKIGIQSTPIQLSVTSEIINTAERALHDH"
     gene            4040..4113
                     /locus_tag="PK937_11415"
     tRNA            4040..4113
                     /locus_tag="PK937_11415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4074..4076,aa:Met,seq:cat)
     gene            4133..4206
                     /locus_tag="PK937_11420"
     tRNA            4133..4206
                     /locus_tag="PK937_11420"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4167..4169,aa:Met,seq:cat)
     gene            complement(4300..5085)
                     /locus_tag="PK937_11425"
     CDS             complement(4300..5085)
                     /locus_tag="PK937_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNH33224.1"
                     /translation="MACGLSKPELQQSFSISYIRNGYFEYNVYKNTFEAYTGRVLVDK
                     PGYQYSVRHIRSISDECTIFDFTDEFYHQIGEKCGNRIAWYLKNKDRSSLLLDCNVEL
                     DYWHSLIRSKIRNSATARLEMDDLIIQLLTAVIGHMSSPATRKVLAEKNIKFHLLAIE
                     KAKEFIAEKFTEDFSLLDVARYCCVSPFHFSRIFKQITSYTPHQYLLSYRLQHAAMLI
                     NSTSLSLTEICYMSGFSRSDYFSAAFSKKYKISPSQFRQANRV"
     gene            complement(5406..5912)
                     /locus_tag="PK937_11430"
     CDS             complement(5406..5912)
                     /locus_tag="PK937_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH33225.1"
                     /translation="MRPGFFILVFFFLISSTYGVHSKTNYLSKSESINMNLTDEETDR
                     KILSALNAQFIKNYITNDTTSHSKLIHKDFVCIEGDGTILNREEYLKGWAHGYDPNIF
                     ISFTYQDELIRIFGNVALVRSKNVYSRMIQGKVVNGSTIYTDTYYKENGQWHCIQAQI
                     TAIKQTSK"
     gene            complement(6092..6745)
                     /locus_tag="PK937_11435"
     CDS             complement(6092..6745)
                     /locus_tag="PK937_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33226.1"
                     /translation="MQLKSRLTQAELLSTAIFTTDRAIFKQQDLIRNKIGELMSLMDD
                     EVRVFLTQIEGGFETRTDTMKIRLYGMLQEKLTFQILTLSVPDIQPERIRFFINDEPE
                     VLKLKSEFMMDQYNKLLNYAMILESAMRLIKNDRELIRRAFLITRDNTFLSIKGHPSR
                     SHRLPLTRQLLESDETAELKISKIALEIEEIEKLARHAKVISHEIDKITLEVSGETD"
     gene            complement(6981..8375)
                     /locus_tag="PK937_11440"
     CDS             complement(6981..8375)
                     /locus_tag="PK937_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH33227.1"
                     /translation="MSPSKSLRILVIEDNATMREGIVDALMLHGLNAEAVSGGKEGLE
                     RMNRYPADLVITDYKMSPVDGMSVLKQVASQFPDTEVMMITAFGTVDVAVEAMKSGAS
                     DFITKPFSPDELIVKVDKILDGIVRRKHYEKTSEEVAYLRTEIDRVYNFSEIIGDAPS
                     MHGIFEIIRRSADSSSAVAIYGESGTGKELIARAIHYNSQRRDKPFIKVNCAALTETL
                     LESELFGHEKGAFTGAIKTKRGRFELADTGTLFLDEIGEISMNMQVKLLRAIQEQEFE
                     RVGGEDTLRVDVRIIAATNRNLQELIQRGVFREDLYYRLHVLPIVVPPLRERKGDIPR
                     LVDHVIRRVSENLGLANVSVEPGVYAALSSYDWPGNVRELENVIERAIVLSADRKLTA
                     ESFLGLTGKSAPFPIAYDDVEKSGLDEALASYEKTMIIQALNKAEGSKTEAARLLGVK
                     TSAFYYKLEKYGLH"
     gene            complement(8379..9239)
                     /locus_tag="PK937_11445"
     CDS             complement(8379..9239)
                     /locus_tag="PK937_11445"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNH33228.1"
                     /translation="MIRWFVLFSMLVVASGSSAFSQKVTANVKIIRDKLQGRNLDLFE
                     GMEQKITSYVNNYDWSKTGDNTPMPVDIQIFLEKVSEVNELRRVHATMFFSNGKVMQS
                     LDKECIFSIRKGAVFYHDENVIDPFLSIIDFYIYIMLGDEMDSLGKLLGTPFFQKARS
                     LATQAKAFVPENVSGWDARLLKSDDFLDLRYQEYRIMKDHFYEAVSQLENEDAAGARQ
                     NATKALDILERIYTKNITSYHSERFIALNYLDFCKIFARSGEKKIYDRLIALDPKNKD
                     TYLKYRNEQN"
     gene            complement(9247..10038)
                     /gene="fadH"
                     /locus_tag="PK937_11450"
     CDS             complement(9247..10038)
                     /gene="fadH"
                     /locus_tag="PK937_11450"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HNH33229.1"
                     /translation="MDGKVVLITGGGTGLGKSMAEHCAARGAHVIISGRRMDVLESAA
                     QDISRAGTRVLTVPCDVRVPDQVQAMTDKAVKEFGRVDHLINNAAGNFLCAAEKLSVN
                     GWNAVINIVLNGTFYCSSSVGKTMIQNGGGTITNIVATYAWASEPGVIHSASAKAGVL
                     AMTRTLAVEWARYGIRVNAIAPGAIRTEGTDKNLWGDPEQRSRMTGRIPMGRFGQPEE
                     VAHTLLFLLSDFSRYITGEVITVDGGAWIGKGTYEMIDVKNINQL"
     gene            complement(10070..10795)
                     /locus_tag="PK937_11455"
     CDS             complement(10070..10795)
                     /locus_tag="PK937_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNH33230.1"
                     /translation="MIAHLVTLQSIDTKLGEIEMLRGDLPKIVEQLKAELEELRDEIN
                     SDKQSVANFEKEKSDLTNEQSMNQEKLKKFQDQLYKATSNKEYEATSAQIDFCEQELN
                     RIKLRSIDIDAQVMELQEGVKPKEERLAGLEKDFSEREGELQTRIEETSKEENELRQK
                     REGLKPQIRPDLINKYERIRKAKNGIAVAPLVKEASCGGCFQQIPPQIIIELKKREKL
                     TNCEYCGRILYIESDVVKEASAS"
     gene            complement(10951..11748)
                     /locus_tag="PK937_11460"
     CDS             complement(10951..11748)
                     /locus_tag="PK937_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH33231.1"
                     /translation="MSEKVLVQYEVKDRVAILTLTDPPANTYTHEMMRQLDECILKAR
                     FDENVNVIVLTGLGEKFFCAGANIDMLSKADPVWKYYFCLHANETLLRLEHTPKLVIA
                     ALNGHCVGGGLEIALAADIRIARKDAGMIGLPEVALGVLPGTGGTQRFARAVGRSLSI
                     ELMATGRKFAYEEALDMGLVNYIYEGNGASFMEQVMAYAKQFTIPNKAAMAVGHIKRS
                     VQTGIEVPLEQGLSLERELQSALFKSGDAKEGLMAFVEKRTPKFTGR"
     gene            complement(11771..12328)
                     /gene="folE"
                     /locus_tag="PK937_11465"
     CDS             complement(11771..12328)
                     /gene="folE"
                     /locus_tag="PK937_11465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNH33232.1"
                     /translation="MKTQELITQLLRNYGENPDREGLRDTPDRVAKSFEFLMGGYQES
                     AEAVIQSAIFEENFNEMVLVRDIELYSLCEHHMLPFFGKCHIAYIPKGKIIGLSKLPR
                     VVDVFSRRLQVQERLTMQIANAIQDALHPEGVGVIIEAQHLCMMMRGVEKQHSLTTTS
                     CMLGVFKDDARTRTEFLTLAHSQNK"
     gene            complement(12529..13287)
                     /locus_tag="PK937_11470"
     CDS             complement(12529..13287)
                     /locus_tag="PK937_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNH33233.1"
                     /translation="MNINKRILIGLGTVVLMVFGVMLYPRFAEYRLRTQLEKENEAAL
                     KEISKTDIIHAAAVADTMPVSFPEKAYLEVPFYCQAPHMNPDSWKIHKESCEEAAVLQ
                     AVFYNKGIKDVSVDSVDWLIKDMLRWQDQHFGGHKDIHADSVKMMMMGYFGYKDEEVK
                     ILRKAKIEDIKRYVAMGYPVIAPTFGRTLKNPFYTPPGPRYHMLTVIGYTPDRVITND
                     VGTRRGKDFTYPYDIFQKSMDEEGGDCLVIMAKK"
     gene            complement(13290..14048)
                     /locus_tag="PK937_11475"
     CDS             complement(13290..14048)
                     /locus_tag="PK937_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNH33234.1"
                     /translation="MKTLVIVVVCLFGILAGWGVSAQSPSSDIKNDGPKTSSVIASVV
                     QAMKDSLSKIPPEKALLDVPFFCQAPFMNMDSWNIHRESCEEAAALQVVYYLRGVKKV
                     DLSATDKILRDMIDWQMKQFKGHHDIRCDSTKTMIMSFFGYTDDEVRIIRKATIQDIK
                     DQILAGNPVIAMANGYVLNNPHYVENLRARGMSGYHMLTVKGFDSEKIITNDVGTMRG
                     NGYTYTYDIFQKAMDFHGGDIIVILPKDRTKTGQ"
     gene            complement(14104..14685)
                     /locus_tag="PK937_11480"
     CDS             complement(14104..14685)
                     /locus_tag="PK937_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNH33235.1"
                     /translation="MHGWCTCAAIGPGLSKNPAIAEWMFPLLSQWSVPSILDADALNI
                     LNGQWKVLRDVHSDMIITPHSGEFRRLFGFEKNSATAMNDELHDWQKKLNHTILLKGV
                     PTQIAGKSHVYVTHTGNPGMAKAGSGDVLTGVIAGLMAQGLDSETAGFCGAYIHGLAG
                     DIAVTDKTEYGLLAGDIIESIPQALQRITNCNS"
BASE COUNT         4062 a         3460 c         2917 g         4290 t
ORIGIN      
        1 cgccatcatc agccttaatg cttataaaac aaccctgcgg cataacccac gacttcttcc
       61 aaatccccgc ttacatgacc ggaatgccta tacataagaa tgcgactatc ggtaatgccg
      121 ttttttttca tagcccgcat cactgcgagt accggcccgt aaccacaggc ctctgttttt
      181 cgggttttga tattggtgta caatgcatca tcgtccatgg cggtgacatt ttctaaaaaa
      241 atagaatcct ttttttcgcc ctccacggcg ttataaaaat gcgacaaatc gctgctggcg
      301 accaataaaa cgccgggatc gcctgcaaac ggagttaatg cttcgctcaa agcatgaatg
      361 gtttcgttgt cttgtgttcc gaataccagc ggtatgatcg aaaattcgtt ttttaaaaca
      421 acctgcaaaa aaggtaattc cacttcgatg ccgtgttcgt cgcgatgccc gagtatattt
      481 attttacaga gagcacacct atctaagata cgatccaccg cttctagtgc gacgggtaca
      541 tctcccaaag gggtacgata actttcccga ataacagaaa tgccatggaa ataatcatga
      601 tgactgggac tgagaataag caccgtatgg tacgacatgc cgcggatagt cgcgaacgct
      661 tccgccgcaa cgtaaccgct ataacgataa ccggcgtgcg gtacgataag tccgcgcggt
      721 tgcccgcctg aaaattgggt tgtagtatta tctaataaac cggatacttc gtcgcgcagc
      781 ttggcaggat cggcctggta aaaaagccct gctacagcgg gtcggcgcac atgtttattc
      841 gttaccgaca taatagaagt atgtgttatg aactttcgcc tgtagaataa tccttgatcg
      901 gcaatccgta tttttcagct ttacgatata tcgtagcacg gccgatattg agacgtcttg
      961 ctacttcaga aatattccca tcgcaatcgg caaaacattt tttgatgatt ttctcttcga
     1021 cgcgttctaa gatttcttcc atcgtaccgg tcatatccat gtacatttct ttaccataac
     1081 ccggtttagg cgcgctgagc gttcggatat tgggtggcag atcttccagc gtgatcgcgt
     1141 tacccgacgc caataccatc gcacgttcca tggagttttc gagttcacgg acattccccg
     1201 gccaatgata ccccatcaaa agctccaaaa cttcgcgtga cataccggag atattttctt
     1261 tcttctcgcg tttcttaaat ttttccatga agtgtgttgc caacagtgcc acgtcttctt
     1321 tgcgctcacg aaggctcggc atcacgatcg gaaaaacact gagacggtaa aacaaatctt
     1381 cgcggaagcg accgtttttt acttcttctt cgaggttttt attggtcgcc gaaatcagac
     1441 gtacatctat tttgatcaca tcgttgccgc caatgcgaga aacttcacgt tcctgaatca
     1501 cacgcaccag tttggcttgc gtcgccatac tcatctcgcc gatctcatcg agaaataaag
     1561 taccgccgtg tgcaaattcg aatttaccga tacgccgctc tgttgctccc gtaaacgcgc
     1621 ctttttcgtg cccaaaaagt tcgctttcca gcagatgctc cggtatcgcc gcacagttaa
     1681 atgcgacaaa tggttttttc tcgcgcgatt tatcgtgctt gtgtatttca cgcgcaacta
     1741 attctttgcc ggtaccgctt tcaccggtga taagtaccgt cacgttactg ctggcaacga
     1801 tcttatccat cagatcgtaa atgcgtcgca ttgcgggact gctgccgacg atcgaaccgt
     1861 agtcgcgagg tccgtagagt tctttttgca gtcgttcgat ttcatcatcc ttggtctttc
     1921 gttcaagggc attacgcacc gacactttca ggcgattgga atcgaacggc ttggtaacaa
     1981 aatcatacgc gccggtttgc atcgcgtcca cagccgactg gatcgtacca tgcgcgctca
     2041 tcatgatgat cggtatttta ggataataaa tacgcaggct tttcatcact tcgatgccgt
     2101 ccataccggg aatacgcaaa tcaagcaaaa tcacatcggg tatatcctga tccaccatgg
     2161 caaaacactc tttgccgtcc gtggcattgt aaatgcgata ctcgtcattt tccaaataag
     2221 cctgaatcaa tcgaaggata ttcttatcat catccacgac aagaattttc ttcttttcga
     2281 cggccgaatc actcatagta agaacctcat tggcgtttta atcgcaaagc gatggcggca
     2341 agttcatctg ccgcagcatc atgtactggt ttcataaacg gttttaagta aaaaaaacga
     2401 aaaccggacg gcacccattc tttttttact ttgatcgcgt ccttttgact ggtcaggaca
     2461 atatagtcat tcgtatcgga accttgcaaa gataaaatgc gtcgaatatc tgcccgtgaa
     2521 taccaatgat gatcatgaaa ttccgcaaaa tgtatgactt ccgcagaatt atcacgagca
     2581 atagactcaa ctgtcctcaa aaacgcgtca ttatgaccaa gcccggccaa tacgattatt
     2641 tttttgtgct tcaaaatttc aaagttcaaa gggtgatcat caagatcata catcgcatac
     2701 cggtaatagg taaatcccaa aaaaggcaat gcgcccgcct ccgtttgcaa taccgcatga
     2761 ggcggtattt tagtaacgac atgcgcattg cattttttaa tattttgttt aagatcgcgc
     2821 aatcggccca acggaagcca tagttgtggg tagtcccttt cccaataatc ctccacacat
     2881 agtacggcat ctttgtaaac ggaaaaatcc tgaaacccat catctaacaa aataatgtca
     2941 gggtcataat ttttttcggc aaaatcaatt ccatgtgaac gattgggcga cgaaataaca
     3001 ggaatattga gattatcatt caaaaaatta attgccatca tatacgcctc atcgcccgat
     3061 gcatcaagtg aagcgttcat ttttatcccg tcacttacgc agacaaggcc gtgcgatttt
     3121 tttcgataac cattggaaat aacgcacagt ttcagaccac gggcaactaa gagcctcgca
     3181 taccattcga caaacggcgt ttttccgctt cccccgaccg ataaacttcc gatgcaaatc
     3241 acgcgaactt tgtttttaac gggtcgaagt ttagcaatcg aattttttat caataataga
     3301 aaagcgaaca ataacgaaag aggaaaaaaa agaatatatg ttatataccg gagtaaactg
     3361 ttcttccact gaaccggtgg tttaatgatc atgcagtgcg cgctccgcgg tgttaataat
     3421 ttcagacgta acggacaact gaatcggcgt actttgtatg ccaatttttg aaaaaggttt
     3481 gggaatttgc gcacgatccc atgaattcag gttccaggca ttcgtcgtat gcaacgatac
     3541 ggcgtgtata tatcgtgtat gcatttgcgc gagagaagca aagcctggtt tacatgttcg
     3601 ggccggtcct ttgggaccgt ccggagttat taaaatccaa tgatttttgt attttaaaga
     3661 gcgtaaagcg agcaatgctt caaaaccgta acgcgaactc gacccttcga ccacagtgaa
     3721 tcccaaacgt tttagcacgc atgcaagttt ttgaccgtct tcgctagcac tgaccaaggc
     3781 aacacactga tcagatttat tatgtacgac caaccaggcg ataaccgaaa aaatatgggc
     3841 atgccaaaat agtatcacac cgggttcagc taaaatcgca ccgtgcggtg tcttaaatct
     3901 taaagtccga aaccatatgc gtaataaccc ggttactact ccacaaactg ttttacgaaa
     3961 catgaataat tttaataacc cgatttattg cttgacatta agatctttaa tctctattat
     4021 tggcgcactt aaatgtgatc gcggggtgga gcagcctggt agctcgtcgg gctcataacc
     4081 cgaaggtcgt aggttcaaat cctgcccccg ctactaaaat atcttactac ccggtggggt
     4141 agctcagttg gttagagcac aggattcata accctgaggt cacgggttca actcccgtcc
     4201 ccactacccc aaaaacccct cagcaatgag gggatttttc gttttaataa tattataaac
     4261 ggcacttctt cctttgtttt gcaattcttg tgtggtaacc tatacacgat ttgcctgcct
     4321 aaattgagat ggtgaaatct tatatttttt agaaaacgcg gctgaaaaat aatcacttcg
     4381 gctaaatccg gacatatagc atatttcagt caacgacaac gaagttgaat taataagcat
     4441 agccgcatgt tgtagcctgt agctaagtaa atactgatgc ggagtgtacg aagttatttg
     4501 tttaaatatc ctactgaaat gaaaagggct aacacaacaa tatcgcgcaa cgtccaatag
     4561 cgaaaaatct tcagtgaatt tctcagcgat aaattctttt gctttttcaa tggcgagtag
     4621 atgaaatttg atattttttt cggcaaggac tttccgtgtt gcaggacttg acatatgccc
     4681 gattacggcc gtcaaaagtt ggatgattaa atcatccatt tcaagtcgtg cagtagcaga
     4741 gttccgaatt ttactccgga ttaacgaatg ccaataatca agctctacgt tgcaatctaa
     4801 caatagtgaa gaacgatctt tgttttttaa ataccaagct attctgttgc cgcatttctc
     4861 tccgatttgg tgatagaatt catctgtgaa atcgaaaata gtacattcat ccgaaatgga
     4921 gcgaatatgt cttacggaat attgatagcc cggtttatca actaaaacac gtccggtata
     4981 cgcttcaaac gtgtttttgt acacattgta ttcaaagtag ccgttacgta tataactaat
     5041 tgagaaactt tgttggagtt caggcttgga aagtccgcac gccatacatt tacatttgaa
     5101 atctacgatt tgataaaacg tggatgcaaa taaaaggttt gtctcggctt tttttttccg
     5161 ccgttcatcc tgaatttttt ttaacgttgc cgccggttta attaacatag tagtattaat
     5221 ataaaatgat caggcatttc atcaacgata tatttcgact aaccacacaa gacctttgca
     5281 agcaaaacaa actgggaaat atctctgaaa ccttagatta aaattataca aataaaaaag
     5341 ccgaggctgc aaaatggaaa cccacatgcg cctcagctat atacaacaac gcgtcaggaa
     5401 ttaatttatt tagatgtttg tttgattgcc gtgatttggg cctgaataca atgccactgt
     5461 ccgttttcct tataatatgt atccgtatat atcgtgctcc cgtttacaac cttcccttgt
     5521 atcattctcg aatatacatt tttcgaacga acaagcgcta catttccaaa tattcttatt
     5581 aactcatctt ggtaggtaaa cgatataaat atattcggat catatccgtg cgcccagccc
     5641 tttaagtatt cttcgcgatt aagaattgtg ccatcccctt caatgcatac gaagtcttta
     5701 tgtattagtt tactgtgtga cgtagtgtca ttagttatat agttttttat aaactgtgcg
     5761 tttagagcac tcagaatctt tctgtccgtt tcttcgtcag ttaaattcat attaatgctt
     5821 tctgattttg aaagataatt cgttttagaa tgtaccccat acgttgaaga aattaaaaaa
     5881 aagaaaacca agataaagaa gccgggacgc ataattatac tccttgattc gtttaataat
     5941 taaataacct ttaattattc tacaacatcc ttagaagatc agctttagaa atcttgcttt
     6001 tctcattatt tgaatagaaa gcgcggaagg tagttttttt aacgggctac aataaagaca
     6061 attcttactt attctttcgt agtgcgtaga gttaatcggt ttctccacta acttctaaag
     6121 ttattttatc gatctcatga gaaataactt tggcatgtct tgcaagtttt tcaatttctt
     6181 caatttctaa tgcaatttta ctgatcttga gttcagctgt ttcatcactt tccaataact
     6241 gtcgcgtcaa tggcagccta tggctccggg atggatgacc ttttatggat aggaacgtat
     6301 tatctcgtgt gatcagaaat gcgcgccgta taagttcgcg atcattttta attaatcgca
     6361 tggcggattc taatatcatt gcatagttta ataatttatt atattgatcc atcataaatt
     6421 cgcttttcaa ttttaaaacc tcaggttcat cattaataaa aaaacgaatt ctttcaggtt
     6481 gaatatcagg aaccgacaat gttagaattt gaaatgttaa tttttcttgc aacataccat
     6541 agagacgaat tttcattgta tcggtacgcg tttcaaaacc gccttcaatc tgcgtaagaa
     6601 aaacgcgcac ttcatcatcc atcagggaca tgagttcacc gatcttattt cggataagat
     6661 cctgctgttt gaaaattgct cggtctgttg taaatattgc agtagaaagc agctccgcct
     6721 gggtaaggcg gcttttaagc tgcatgcggt cgaatatatc aattgtcggg tgttgtttga
     6781 tgcctaaaat ttcacgaccg agactatcgg cctcggattc taaaatttca cgttggatat
     6841 gaagactttg aataatcgtt gaagcttgaa taatatcggt ttcaatccgg cgaataagcg
     6901 atgagctata cgcagactgc agactgtctt gcgccgatag agccgtaacg catgaccaaa
     6961 gcaaaaaaag aattactttg ttaatgaagg ccatattttt caagcttata ataaaaggcg
     7021 cttgttttga caccgagcaa ccttgcggct tccgttttac ttccttcggc tttatttaat
     7081 gcctgaatta tcatggtttt ttcatacgac gccaacgcct catccaaacc tgatttttcc
     7141 acatcgtcat aggctatggg aaatggagcg gatttaccgg tcaatcctaa aaaggattcc
     7201 gccgtcagtt ttcgatcggc cgatagcaca atagcacgtt caatcacgtt ttccaattcg
     7261 cgtacatttc ccggccaatc gtaactgctt aaagccgcat agacaccggg ttccacggaa
     7321 acatttgcta aaccgagatt ctctgataca cgtcggatca catgatctac caggcgcggt
     7381 atatcccctt ttcgctcccg taacggaggt acgactatcg gtagcacatg caaccgataa
     7441 tatagatctt cgcgaaatac tccacgctga atcaattcct gcaaattacg atttgttgcc
     7501 gcgataatac ggacatccac gcgcaatgta tcttcccccc ctacgcgttc aaattcctgt
     7561 tcctgaatcg cacgaagaag tttgacctgc atattcatcg aaatttcgcc gatttcgtca
     7621 aggaaaagtg tcccggtatc cgctaattca aatcgtccgc gtttggtctt aatggcaccg
     7681 gtgaaagcgc ctttttcgtg gccaaacaac tcactttcta aaagtgtctc ggttagcgcg
     7741 gcacaattaa ctttaataaa aggtttatca cgacgctgac tattgtaatg aatggcgcgc
     7801 gcgatcaatt cttttccggt accgctttct ccatatatcg ctacggcgct ggatgaatca
     7861 gcactgcgac ggattatctc aaaaatgcca tgcatgctcg gggcatcccc gataatttca
     7921 gaaaaattgt aaacacgatc tatttccgta cgaagatatg cgacttcttc gctcgttttt
     7981 tcataatgtt tacgacgtac gatcccatct aaaattttat caacttttac aatcagttcg
     8041 tcaggactga aaggtttggt gataaaatca gaggctccgg atttcatcgc ctccacagcg
     8101 acgtccactg taccaaaagc tgtaatcatc ataacttcgg tatcgggaaa ctgtgacgcg
     8161 acctgtttga gaacggacat gccgtccacc ggtgacattt tgtaatctgt gatcaccagg
     8221 tcggccgggt agcgattcat gcgttccaac ccttcttttc caccggatac ggcctctgcg
     8281 tttaatccgt gaagcatcag cgcatccacg atgccttcgc gcatcgtcgc attatcttcg
     8341 atcacgagta tgcggagaga tttggacggc gacatgaatc agttttgttc gttgcggtac
     8401 ttgaggtagg tgtctttatt ttttggatcc aaagcgatca aacgatcata tattttcttc
     8461 tcaccggagc gcgcgaaaat tttacaaaaa tccaaatagt tcagcgcgat aaatcgttcc
     8521 gaatgatacg atgtaatatt tttagtgtaa atccgctcta agatatccaa cgctttggtt
     8581 gcattttgcc gtgctccggc cgcatcctca ttttctagct gcgatacggc ttcatagaaa
     8641 tgatctttca taatccggta ttcctggtaa cgcagatcga gaaaatcatc ggatttcaat
     8701 aaacgtgcat cccaaccgga aacattttca gggacaaatg ctttagcctg cgtagccaaa
     8761 ctccgcgctt tttggaaaaa aggcgtacct aataatttgc caagcgaatc catttcatca
     8821 ccgagcatga tgtagatata aaaatctata atacttaaaa aaggatcgat gacattctca
     8881 tcatgataaa aaacggcgcc ctttcggatc gaaaaaatac attctttatc cagcgactgc
     8941 atgacctttc cattggaaaa aaacatcgtg gcatgcacac gtcgtaattc atttacttca
     9001 gaaactttct caagaaaaat ctgaatatcc acaggcatcg gtgtgttatc gcctgttttg
     9061 gaccaatcgt agttattgac atacgaggtg attttctgtt ccattccttc aaataaatcg
     9121 agattacgtc cctgaagttt atcacggata attttgacat tggccgtcac tttttgtgaa
     9181 aaagcagacg aacctgatgc cacaaccaac atgctgaata gaacgaacca tcgtatcatg
     9241 atcatattat aattgattaa tattttttac atcaatcatt tcgtaggtgc cttttcctat
     9301 ccaggcaccg ccgtcaaccg taatcacttc tcctgttata tatcgggaga agtctgaaag
     9361 taaaaacaaa agtgtatggg ccacttcttc gggttgcccg aaccgaccca tcggaatacg
     9421 accggtcata cgtgacctct gttctggatc accccagagg ttcttatcgg taccttccgt
     9481 acgaattgct ccgggtgcta tcgcatttac acgaataccg tagcgagccc actcgacagc
     9541 gagcgtacgc gtcattgcca gtacccctgc tttggcgctg gccgaatgaa tgacgccggg
     9601 ctccgatgcc caagcatacg ttgcgacaat attggttatc gtaccgcccc cgttttgtat
     9661 catcgttttt ccaacactgc tcgaacaata aaacgtacca ttcaaaacga tattgatgac
     9721 ggcgttccaa ccgttgacgc tcaatttttc agcagcacac aaaaaatttc ctgcggcatt
     9781 attgattaaa tgatccacac ggccaaattc tttaacggct ttatcggtca tagcttgtac
     9841 ctgatccggt acgcgtacgt cgcatgggac ggttaacacg cgtgttcctg ctctggatat
     9901 atcctgcgcc gccgattcca gcacatccat ccgtcgaccg ctgataatga catgcgctcc
     9961 tcgcgctgca caatgctcgg ccatactttt gccaagccct gtaccgccac ccgtgatcaa
    10021 aacaactttg ccatccaaaa atttttgttc cagcatagcg ccccgataat tatgatgccg
    10081 acgcttcttt gacgacatcc gattcaatgt acaaaatgcg tccgcaatat tcgcaattcg
    10141 tcaacttctc acgcttcttc aattcgatga tgatctgcgg tggtatctgc tgaaagcatc
    10201 ctccgcacga cgcttctttg actagcggcg cgacggcaat accgtttttt gctttgcgaa
    10261 tacgttcgta tttattgatc agatcagggc gaatttgcgg ttttaagcct tcgcgttttt
    10321 gacgcaattc gttttcttct ttactggtct cttcgatacg cgtttgcaat tcgccttcgc
    10381 gttcagaaaa atctttctcc agtcccgcaa gacgttcttc tttaggtttt acaccttcct
    10441 gaagttccat aacctgcgca tcaatgtcta tcgaacgcag cttaatgcgg ttcaactctt
    10501 gctcacaaaa atcaatctga gccgatgtcg cttcgtattc tttattggat gtggccttgt
    10561 ataactgatc ttgaaatttt ttgagtttct cctgattcat ggattgctca ttggtcagat
    10621 ctgatttttc tttctcaaag ttggccacgg attgcttatc cgaattgatt tcatcgcgga
    10681 gttcttccag ttccgctttg agttgctcca cgattttagg taaatcgccg cgcagcattt
    10741 cgatctcacc taatttggta tcaatgcttt gaagcgttac cagatgggct atcatatttt
    10801 gcaaagttct actcctttat caaaaaaaaa tgcactaatt aagattagtg catacgctat
    10861 aaaaaagaaa gaccggcgca cccgttccgc attgtgcggt cggtactttg gaagtattcg
    10921 ggtgcgctga tctcgtaaat acattgcagg ttaacgtcct gtaaatttag gagtacgttt
    10981 ttcgacgaaa gccatgagtc cttctttggc atcaccgctt ttgaaaagcg ccgattgcaa
    11041 ttcgcgctca agggagagtc cttgttcgag cggaacttca ataccggttt gtactgagcg
    11101 tttgatatga ccgacggcca tcgcggcttt attgggaatc gtaaactgtt tggcgtacgc
    11161 catcacttgt tccataaacg aagcgccgtt gccttcatag atatagttaa caaggcccat
    11221 atcgagcgct tcttcgtaag caaatttacg tccggttgcc atcaattcga tagaaagcga
    11281 acgtccaacg gcacgtgcaa aacgctgtgt gccgccggta ccgggtaata cacccagcgc
    11341 cacttccgga agaccgatca taccggcatc tttacgcgca atgcgtatat ctgcagccaa
    11401 tgcgatctct aaaccgccgc caacacagtg accattcaat gccgcaatga ccaacttcgg
    11461 cgtatgttcg agacgcaata acgtttcatt ggcatgaaga cagaagtaat acttccatac
    11521 aggatccgct ttgctaagca tgtcaatatt ggcgccggcg cagaaaaatt tctcacccaa
    11581 accggtaagc actatgacgt tgacattttc gtcgaaacgc gctttgagaa tgcactcatc
    11641 caactgacgc atcatttcat gcgtataggt attggccggc ggatccgtca gcgtcaatat
    11701 ggcgactctg tcttttactt cgtactgtac taatactttc tcggacatgg tactgctctc
    11761 cctgaaatgg ttatttattt tgtgaatgcg ctaatgtcaa aaattcggtt cttgtgcggg
    11821 catcgtcttt gaatacgcca agcatacaac tcgtcgtcgt aaggctgtgt tgcttttcca
    11881 cgccccgcat catcatacaa aggtgctgcg cttcgatgat cacgccgacc ccttcgggat
    11941 gcaaagcatc ctgaatagca ttagcgattt gcatggtcag gcgttcctgc acctggagac
    12001 ggcggctgaa tacgtcaaca actcgcggta acttgctgag accgatgatt ttccctttgg
    12061 ggatataagc tatgtggcac tttccaaaaa aaggcaacat gtggtgctcg caaagactgt
    12121 acaattcgat atctcgaacc aataccatct cattaaaatt ttcttcaaaa attgccgatt
    12181 gaatgactgc ttcagcgctt tcctgatagc cgcccataag gaattcaaaa ctcttcgcca
    12241 cacggtccgg cgtatcgcgt aaaccttccc gatcgggatt ttcaccgtaa ttacgaagca
    12301 gttgtgtgat cagttcttgt gttttcatgg tatcaaataa aaaaccctga attcttaaca
    12361 attcagggta aaaatttaag caaaccgttg agcggtaact gtgcgttcaa atgtcgtctt
    12421 aaaattaaaa agtacgctca aaacaatcat gattttagtg aacagttgtt ttttaaaaac
    12481 agttgaaaat agacgcctgg cacctataat gcaagcaaaa tttatagatc attttttggc
    12541 cataatgacc aaacaatcgc cgccttcttc atccatcgat ttttgaaaaa tatcgtacgg
    12601 ataggtaaaa tcttttccgc ggcgagtgcc gacatcgttg gtaatcacgc gatcaggtgt
    12661 atatccgatc accgttagca tatgatacct cggccccggc ggggtataaa aaggattttt
    12721 aagcgtccgt ccgaacgtcg gcgctatcac aggatacccc atcgcgacat aacgcttgat
    12781 gtcttcgatt tttgctttac gtaaaatttt gacctcttcg tctttgtagc caaaataacc
    12841 catcatcatc attttgacgc tgtcggcatg aatgtctttg tgtcctccaa aatgctgatc
    12901 ttgccaacgc aacatatctt tgatcaacca atccacagaa tctacagaaa catctttgat
    12961 gcctttatta tagaatacgg cttgcaaaac ggccgcttcc tcacagcttt ctttatgaat
    13021 tttccaactg tccggattca tatgcggcgc ctgacaataa aacggtactt caagatacgc
    13081 tttttcagga aacgaaaccg gcatggtatc cgctacggca gcggcatgga tgatatccgt
    13141 tttagaaatt tctttaagcg cagcttcgtt ttctttttcc aattgtgtac ggaggcgata
    13201 ttcggcaaaa cgcggataga gcatcacacc gaaaaccata agaacaaccg tacccaatcc
    13261 gatcaaaatt cgtttattga tattcatatt tattgccctg tcttagtacg atcttttgga
    13321 agaatcacga taatatcacc gccgtgaaaa tccatcgctt tttggaatat atcatacgta
    13381 taggtataac cgttaccgcg catggttcct acatcgttgg tgataatttt ttcagaatca
    13441 aaacctttta ccgtcagcat atgataaccc gacatgcctc gggcacgaag attttcgaca
    13501 tagtgcggat tgttgagcac gtaaccattg gccatcgcaa tcacaggatt acctgcaagt
    13561 atctgatctt ttatatcttg aatagttgct tttcggataa tgcgcacttc gtcatcggta
    13621 taaccgaaga atgacatgat catggtttta gtactgtcac aacgaatatc gtgatggcct
    13681 ttgaattgct tcatctgcca atcaatcata tcacgtaaaa ttttatccgt agcgctcagg
    13741 tcaacttttt tgacaccgcg caaataatag acgacttgca gggccgccgc ctcttcacag
    13801 ctttcgcggt gaatattcca gctatccata ttcataaacg gtgcctggca aaaaaacgga
    13861 acatccaaca gagctttttc aggcggtatt ttactaagcg aatctttcat agcttgcacg
    13921 acagatgcga taaccgatga agttttcgga ccatcgttct taatatcgga ggacggcgac
    13981 tgcgccgaaa caccccatcc ggcaagaatg ccaaacaggc acactacaac gatcacaagt
    14041 gttttcatac accacatttc ttgaatgata gactgtaaga tcagggaaaa tataaaaaaa
    14101 caatcaggaa ttgcaattgg taattcgttg caatgcttgc ggtatcgatt cgatgatatc
    14161 gcccgccaaa agcccgtatt ctgttttgtc tgttacggca atatctccgg ccaagccgtg
    14221 aatatacgct ccgcaaaagc cggccgtttc cgaatctaat ccttgcgcca tcagccccgc
    14281 gataactccc gtcaatacat caccgcttcc tgctttggcc atacccggat taccggtgtg
    14341 tgttacataa acatgcgatt taccggcgat ttgcgtcgga acccccttga ggagaatcgt
    14401 gtggtttaat tttttttgcc aatcatggag ttcatcattc attgcggtgg cgctgttttt
    14461 ttcaaaacca aataaacgac ggaattcacc gctgtgcgga gtgatgatca tatctgaatg
    14521 tacatcgcgc aaaactttcc attgcccgtt taaaatattg agagcatccg catccaaaat
    14581 cgaaggaaca ctccattgac tcagcaatgg aaacatccat tcagctatcg cgggattttt
    14641 agatagaccc gggcctatag ccgcacaagt gcaccatcca tgcaaatcaa caatcaattt
    14701 tacacataag tatgatcgtt atgttatga
//
LOCUS       DAOQGF010000086        14656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1889_length_14656_cov_7.148004, whole genome shotgun sequence.
ACCESSION   DAOQGF010000086 DAOQGF010000000
VERSION     DAOQGF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_14656_cov_7.148004"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..4213)
                     /locus_tag="PK937_11485"
     CDS             complement(422..4213)
                     /locus_tag="PK937_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33236.1"
                     /translation="MRLFLSLLVLLFAISITAQDIPPRITIYNPPGAHPQSFELTQDA
                     SGIIYAVNYNGIFGFDGVRWKKLAGRLETYDWNGITADKKTGRIYIGGFQYLGYVAMD
                     SGRSRTVVLNSIVPGDTPVDLVRNIVIRENDVFFIDRSAIFRYRDGIITSQKPKFVFG
                     TGDKFGDRIFINDFGPTEQRMLELVGDSLVVLPYFKNEARIHCLIPAGEGKFIASTGS
                     GIYAWDHGREYPIYQSKDVRRGIRLSDGGFAFRTRSEGVIILDSALRRVEIINEDVGL
                     SANSTRDVMEDRFGNLWVATEDGINKIDRSSLKILQKAQGLKGCAYKIAPMGDLNYIG
                     TLNDLYLIQNGKVVPQNIGRINGGVALAKGYNFASGRGIIHLKKNNNLQLIPNSPDAI
                     SIVALDERYLVLGKRLGITFYDVISNRETPTTAASKISGIGQLWYDRRGSVWAESEYG
                     NTYRIDMKDTSLHVYEGDWKMLLCADSLVFMQHKDTIFRYDFIRKSFIPNPSFFPESS
                     NAVKAIADNAGDIWFFTNTAVWNGHYKETQWHWEKDPYYRIHNHTKQFPPNEVYRDAR
                     GMIWFLTDDGVLRIGPEMVSQRQSFDVVISRFSVSNDSALIINDRRTVPITLPYALNS
                     VRIEYGATSFIDENNTIFQTWLEGFESVPSGWTSDPVSVYSNLSEGEYVFHVSAMNAV
                     RHVQTTELRFRVLPPWYRTWWAWLTYLMLFVVVVTGLVRWRSYQIMREKERLERIVEE
                     RTHLLSVQTQKLEALDAQKSRFFANISHEFRTPLTLLQGPLETMKEKPADDSTIQSML
                     RNTRRLLRLVNQLLDLSKIEHRQMKLRAYRGTWDFIKPAVFSFESLANKKGLSFTLDV
                     SPDLPVMYRDRDVIEKILFNLLSNAIKFTNEGGVSVKVYVNEGALTIDVEDTGIGISE
                     TDLVLIFDRFYQADHSGARAYPGTGIGLALAKELAHLHHGSLTARSRQGKGTVFSFTI
                     PIIEQSIPEDERTESETHSQESAPEWDDVTGPIQSATSADKDVDKNTLLIVEDNDEIR
                     QYIHGIVSNDYHVIEAADGEDGMAEALRRTPDIIISDVMMPRKDGITLSRELKSDRRT
                     SHIPLILLTAKADTEDKIEGLETGADDYLVKPFNEKELRTRVANLIQSRKLLREKYAK
                     EILIKDQDFEGETPDDLFLKELTQLIQSNLSDPEFGVEAISARLAMSRSQVYRKLVAL
                     TDTTPSELIRKLRLRKAKSLLESQAGNVTQVAYEVGYTSLSHFTKAFRDEFGINPSDT
                     I"
     gene            complement(4334..4906)
                     /locus_tag="PK937_11490"
     CDS             complement(4334..4906)
                     /locus_tag="PK937_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33237.1"
                     /translation="MKAKNKLWMICLVSIAISSCGGGGGKDKITLTKATDQFATGNYT
                     TALRNFENLIASEGAPAVAGAGWCLVRLSNVARADSFFVTVAADSNTDAYAGWSFSSW
                     ALNDPSEAISRADFVIRKDANFQMSLDTRVTKSHLVWIQASSYLQLGDYTSCFNKIKL
                     LDAAYTYPSGTSVEIATALMQKLQSLGSAS"
     gene            complement(4936..8139)
                     /locus_tag="PK937_11495"
     CDS             complement(4936..8139)
                     /locus_tag="PK937_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33238.1"
                     /translation="MMFFDANNQEKTDFPRVLGTIYDVAYHPQYGWFIAGNFTHINGV
                     KRKYIARIKTDLTLDMNWNPIVDGEVRSIAVNEDFVYFGGNFSKVSDQTRYHIAAVGV
                     ADGVLSDWNPGADGIVYDMIVSSNTLIIGGQFTEIAGEERFSLGSIDISSGNATAWAP
                     HVTYSENNPGIVYAIVVSSNNVYIGGEFFYLGGVERQHCGAVDMASGAVNPFNPWPNT
                     IVFSLYVKNSTLYIGGMFTQIFSQVRNRLAAYSLSLNQLNSWDPNIDSYNTFPNANSS
                     VRAISVLGDKILVGGIFTKVGSTSKANYALFDLNTGNLSSITKTPSGSVFTITTASSD
                     NEAIAGGSFDSFSQYITRYRLAAFDAQTGALRDWAPVVDGAVTSIEVFENTVLIGGYF
                     NKVNNISRPYAASLIGYGATGSGQLNNWAPGLNGIVSQLLIAGESLYIRGSFTSVSEN
                     NRLNIALFDLNTMQLRQTSLQIPYPTSYLTLRAMGAVDSVLMLGIQYFEGNSFKSVLS
                     EFNTRTGQEIRNSPTNKTINAITATSSVIYLGGYFTQVKGVYRSGAAALDRKTGEIKA
                     WDAKLSGGLGLTKMVTAGNYIYMTGDFDSVGAIPQSGFTKIDMISGVCSPMGHTYNGS
                     IAGMDINEQNIYIGGSFSHFNFLSNNDFTVIQDSTILVPPKNPMCSPSLELDRVIDLS
                     WTKSSGATYYKIYRGLSSFFDTTGNCIATVGDVNTYRDTLGGYIKYYYKIGAFSEGNF
                     SKLSAIASATAPDFIAPEANIGLLVPGFGTHVKIVVTSNESLKSMIVNINNSSVTMSQ
                     SGSLFFGGYTIQQAGQLTITATAKDQSNNTTSLEKSFNVQTIGRPFYNDLYKITVSGS
                     DGYFLTTDDDKSFSGSDDLIPLQKPKTFLTTSIPSQIALAFTEYEITALSGLKKRQNF
                     TNSAIGLYAQIGDERILLAKGGDYTNSVVLEKSVQDKLISLLSTCGLTIAAFYNPDFE
                     GIPTEFALSQNYPNPFNPRTTIRYDVAKAGKVVIKVYNMLGQEVATLINAQRDPGRYS
                     IQWDGRNNAGSVVASGVYLYRMQVGDVVRSKRMLFLK"
     gene            complement(8487..9059)
                     /locus_tag="PK937_11500"
     CDS             complement(8487..9059)
                     /locus_tag="PK937_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33239.1"
                     /translation="MKAKNKLWMICLVSIAISSCGGGGGKDKITLTKATDQFATGNYT
                     TALRNFENLIASEGAPAVAGAGWCLVRLSNVARADSFFVTVAADSNTDAYAGWSFSSW
                     ALNDPSEAISRADFVIRKDANFQMSLDTRVTKSHLVWIQASSYLQLGDYTSCFNKIKL
                     LDAAYTYPSGTSVEIATALMQKLQSLGSAS"
     gene            complement(9089..13225)
                     /locus_tag="PK937_11505"
     CDS             complement(9089..13225)
                     /locus_tag="PK937_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH33240.1"
                     /translation="MRYGLQKPFFWLLSAFVIAGILIVGSSEQRSTANMKKKHLKNTP
                     EALKQALLEQKIKRRAEGYAKPDQPEKFDILHREIRTQYGKQLPDYQMGYKNIEMEKA
                     GIIRREGEKYVRNQMMSSSTLSWTSRGPSNFLGRSRGLVVDPDDANHLTYYLGSVGGG
                     IWKTTNGGSYWENLTPEFPNLSTTVIAMASSNHNIMYAGTGEGYQAAVGMIKGDGIFR
                     STDRGASWAQLSATSSNSDFAYVNRLIIDPNNSDIVVAGTNSAIMRTTDGGTNWTKVH
                     TAGNNRIQQVLADPSDFNIQYATAEALGFLKSTDAGITWAPSSTGIGAVDRLEMAIAP
                     SNTNLLYASVESGTNSVLYRSTNGGANWATVADTTGQSASSIAWLGAQGWYDNTIAVH
                     PYDPNILFVGGINIYRINISNSISAGRRGTTRMTNWYPGLSRPSGGTYPYIHADQHNI
                     VMIPISEPNNFWILHTNDGGFARSTDGGQTWLFDEDEAKGIVTAQFYGVDKMPGASAY
                     IGGTQDNGTWMSPVDATVDSSWYAMLGGDGYDGVWKANDPNQIIGCLYDNRLYKTTDG
                     GQTWNSATSGLLDVDNANSAFVTEIGRSKLDPDLLIVPGGSGFWRSEDFGDSWTLAPI
                     TSSYGFSTARNLAEISLADPNIVWAGTNMSAAGKLHVSTNAGKSYTATNNYTAVTLGR
                     VTGIATHPYNPNEAFALFSIADKPKVLRTMDRGQTWTDISGFGANSSSNNGFPDVATY
                     SLLVMPTDTNEIWAGTEIGIFRSTDNGATWAYANNGLPAVSVWELKIIDNEIVAATHG
                     RGIWTAPISAPPLPTVVLGPTLKQAYQNPATGLVNVELGLRNTYDSTIVFMGNTRALT
                     LGSTGINDSTVSFSSTQIGSVSIKASSYRNGTLYETVAKSVTLVQAVARNDYYNAFNT
                     STSANGEFTGSLFSATKPSGFSSFALHTAHPYTNNTNNTFQLNYPITVASDSSMIVFD
                     EIAIVEPGDPGVPFPEPEFWDYVVVEATSDGISWEPVIDGWDSRANSTWLSTFNSVGS
                     GSNSMFKQRRIDLTDKYEPGTKIFIRFRMFTDAASTGWGWCIDNLKINAIETPPTVTV
                     GALASPVINVAKFAVSSNRGMSTAALTAGGQSVALSRLGGSLFFGSYSITSGTVSITA
                     TCSDSVGNTSTTLNKQFTVTPLSKSSSLGGYSVTGSGEGYVVASLVQATDIPSGWIEI
                     GSMYEAGITGTPTNLKITYDLPNESTLDRMIPGSSQRYKIGLYEKDGNQWKLISAESN
                     GKLSGEFSHKAIAAFYNPDFEGIPTEFALSQNYPNPFNPRTTIRYDVAKAGKVVIKVY
                     NMLGQEVATLINAQRDPGRYSIQWDGRNNAGSVVASGVYLYRMQVGDVVRSKRMLFLK
                     "
     gene            complement(13938..14168)
                     /locus_tag="PK937_11510"
     CDS             complement(13938..14168)
                     /locus_tag="PK937_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33241.1"
                     /translation="MNKKMIITIAAIAVIAVGAVYAYRMNTSETCPPGMTGGDCCKST
                     EVKAQEVKSSEQQATASTETHSCCPSGAEKKQ"
     gene            complement(14218..>14656)
                     /locus_tag="PK937_11515"
     CDS             complement(14218..>14656)
                     /locus_tag="PK937_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNH33242.1"
                     /translation="HSLHIRIQQHSRNALYIASELEKLGLRVYYPGLVSHRQHTLLKS
                     MMNAQFGFGGMVAIDAGTSEKAEALMTRMQQEKVGYLAVSLGYFKTLFSAPGHSTSSE
                     IPEEERQAMGLGDGLVRMSVGLDSDIGRSFARIKTCLAELKLI"
BASE COUNT         4305 a         3458 c         2736 g         4157 t
ORIGIN      
        1 caaaaaaatt ctaaaaagaa gaaaaagttc atatactagc tcagaaaata ctatctctgc
       61 actctggaaa tatcaataag cccaatcagc catgtaagta aaaataaaca agggattata
      121 tccaactcga agtcttactg aaaagtattc tcttaaggct atcacattga atctggcaac
      181 aaaaaaatat tctttttgta aaacccgact actcattcga tcgcaaacat caaatacatg
      241 ctcatcacta ataattaata tactagattc acgcaaaatc tgagttaatc cagattaact
      301 cgatcaaatt ttttcttcga aaatttccgg ataagacgag atgcgtacta acgatagtag
      361 gttaattact agcggttttg ttgcaactta tgttgcggag attgttttta aagcttttag
      421 atcagatcgt atccgaggga tttataccaa attcatcgcg aaaagctttg gtaaaatgtg
      481 acaaactggt ataaccgact tcgtaagcga cctgcgtcac attaccggct tgtgattcca
      541 gcaagctttt ggctttccgc aaacgcaatt ttcgtatgag ttcggatgga gtggtatcgg
      601 taagcgcgac taatttgcga taaacctgag agcgactcat ggccaatcgt gcactgatcg
      661 cctcaacacc aaattcagga tccgacaggt tcgactgtat gagttgcgtt aattctttca
      721 aaaaaagatc atccggtgtt tcaccttcaa aatcctgatc cttgataaga atttctttcg
      781 cgtatttttc acgcagaagc ttccggctct gaatcagatt ggctacacgc gttcgcaatt
      841 ctttttcgtt aaacggtttt accagatagt catcggcacc ggtctccaac ccttcaatct
      901 tatcttcggt atcggctttg gccgtcaata gaataagcgg aatatggctg gttcggcgat
      961 cggatttcaa ctcgcgtgat aatgtaatac cgtcttttcg gggcatcatc acatcggaaa
     1021 taataatatc cggtgttctt cgcaaggctt ccgccatacc gtcttcaccg tccgcggctt
     1081 ctatgacgtg ataatcattg cttactatac cgtggatata ttgacgtatt tcatcgttat
     1141 cttcgacaat caacaacgta tttttatcga catccttatc ggcagacgtg gcgctttgaa
     1201 tcggtccagt aacatcatcc cattcgggcg cactttcttg ggaatgcgtc tccgattcgg
     1261 tacgctcgtc ttcgggtata ctttgttcga tgatcggtat cgtaaagcta aaaactgttc
     1321 ccttcccttg ccggctacgc gctgtcaaag atccatgatg gagatgcgct aactctttgg
     1381 ccaatgcgag tcctataccg gtgccgggat acgcgcgcgc acccgaatga tcggcttgat
     1441 aaaaacgatc aaaaatcaaa actaaatcgg tttcactgat accgataccc gtatcctcga
     1501 catctattgt caaagcgcct tcgttcacat atactttaac agatacaccg ccttcgtttg
     1561 taaacttgat cgcattggaa agcaggttaa ataatatttt ttcgatgaca tcgcgatcac
     1621 gatacatcac cggtagatca ggcgatacat ccagcgtaaa cgacaaacct tttttattag
     1681 ctaacgattc aaaagaaaat acagccggtt ttataaaatc ccacgtaccg cgatatgctc
     1741 tcagcttcat ctgacgatgc tcgattttag ataaatctaa aagttggtta actaagcgca
     1801 ggagtcgtcg cgtattacga agcattgatt gtatcgtact gtcgtcggcc ggtttttcct
     1861 tcatggtctc cagcgggcct tgcaacaaag tgaggggcgt gcgaaattcg tgagaaatat
     1921 tggcaaaaaa tcgcgacttc tgcgcatcaa gcgcttctaa tttttgagtc tgaacagaca
     1981 atagatgcgt acgttcctcc acgatacgct ctaaccgttc cttttcacgc atgatctgat
     2041 aggaacgcca acgcacaagc cctgtcacta caaccacaaa caacatcaaa taggttaacc
     2101 atgcccacca tgtccgatac cacggcggca aaacacgaaa ccgcagttcg gttgtctgta
     2161 cgtgccgcac cgcattcatg gccgatacgt gaaaaacata ttccccttcg ctaagattag
     2221 aatacacgct taccggatcg gaagtccacc cgctaggtac cgattcaaaa ccctctaacc
     2281 atgtttggaa tatcgtattg ttttcatcaa taaatgaagt cgccccgtat tcgatgcgta
     2341 ccgaattcaa tgcatatggc aaagtaatag gaacggttcg ccgatcattg ataattaaag
     2401 ctgaatcgtt gcttacactg aatcgggaaa taaccacatc gaacgattgc cgttgtgaaa
     2461 ccatctccgg tccgatacgt agcacaccgt cgtccgtaag aaaccaaatc atcccgcgcg
     2521 catcacgata tacttcgtta ggcggaaatt gtttggtgtg attatgaata cggtaatagg
     2581 gatctttttc ccaatgccat tgtgtttctt tataatgacc attccatacc gcggtgttcg
     2641 taaaaaacca aatatcaccg gcattatcag ctatggcttt gacagcattc gatgattccg
     2701 gaaaaaacga cggattagga ataaaacttt ttcggataaa atcgtatctg aaaatggtat
     2761 ccttatgttg cataaatacc aatgagtccg cgcacaacaa catcttccaa tcgccctcat
     2821 acacatgaag gctcgtatct ttcatatcaa tccgataggt attgccatat tccgattccg
     2881 cccacaccga tcctcgccga tcataccaga gctgtcctat cccggatatt ttggaagccg
     2941 cagtcgtcgg cgtctcgcga ttggaaataa catcataaaa cgtaattccc aatcttttgc
     3001 caagcacgag ataacgctca tctagtgcta caatactgat ggcatccggg ctattgggaa
     3061 tcagctgaag gttgttattt tttttcagat gaatgatccc gcgacctgaa gcaaaattat
     3121 accccttcgc aagagcaacc ccgccattga ttcgtccaat attttgtgga acaacctttc
     3181 cgttttggat caggtacaga tcgttgagcg tgccgatata gttgagatca cccatgggcg
     3241 cgattttata agcacaccct ttcaatcctt gcgctttttg caaaatcttg agcgatgaac
     3301 gatctatttt atttattccg tcctccgtag caacccacaa atttccaaaa cgatcttcca
     3361 taacatcgcg ggtggagtta gcggaaaggc cgacatcttc attaatgatc tctacgcgac
     3421 gcagtgccga atccagtatg atcacacctt cggatcgtgt tctgaatgca aatcctccgt
     3481 cggacaatcg gattccccgg cgcacatctt tggattgata aataggatat tcacgcccat
     3541 gatcccatgc ataaattccg gatcccgtac ttgcaataaa ttttccctca cccgccggta
     3601 tcagacagtg aatgcgggct tcatttttaa aatacggcag gacgacaagc gagtccccca
     3661 caagctccag catacgttgt tccgtcggac caaaatcatt tataaaaatc cgatcaccaa
     3721 acttatcgcc cgtcccaaaa acaaatttag gcttctgtga tgttatgata ccgtcccgat
     3781 aacggaatat agcagagcga tcaataaaaa aaacgtcgtt ttcacgaata actatatttc
     3841 gcactaaatc aaccggcgta tcgcccggaa ctatcgaatt taaaaccacc gtacgggatc
     3901 ggccgctatc catggctaca tatccgagat attgaaaccc gcctatataa atgcgccctg
     3961 tttttttgtc cgccgttata ccgttccaat cgtacgtttc caatcgccct gctaattttt
     4021 tccaacgcac tccgtcaaat ccaaatatgc cattgtaatt caccgcataa atgatcccgg
     4081 acgcgtcttg cgttaattca aaactttggg gatgcgcacc gggcgggtta taaatggtaa
     4141 tacgcggagg aatgtcctga gcggtaatag aaatggcaaa tagtagaact aacaaggata
     4201 aaaaaagacg catactttca aaagatcaat tgcacggcta agatgtacca ataatatgca
     4261 aaaaaaatca gaaacggtca taaaaaaagc aaggttttaa accttgcttt ttttatagtg
     4321 attggaaaga agtttatgaa gctgaaccaa gcgattgcaa cttttgcatc agtgcggttg
     4381 ctatttcaac cgatgtcccg ctcggatagg tatatgccgc atccaatagc ttgatcttgt
     4441 taaaacaaga cgtataatca cccaactgca aataactgct cgcttggatc caaactaaat
     4501 gactcttggt aacgcgagta tccaaactca tttgaaaatt cgcatcctta cgtataacaa
     4561 aatcagcacg tgaaatagct tcactcggat cgtttaatgc ccacgacgaa aaactccaac
     4621 ccgcataggc atccgtatta ctatccgctg ctaccgtcac aaaaaaacta tcggcgcggg
     4681 ctacattgct caatcgaacc aagcaccaac ccgcacccgc gaccgccgga gcgccctccg
     4741 atgcaatcaa attctcaaaa ttacgcaatg cggtcgtata gttcccggtt gcaaattgat
     4801 cggtcgcttt ggtcaaagtg atcttgtctt tacctccacc accgccacac gatgaaattg
     4861 ctatggaaac taagcatatc atccaaagct tattttttgc tttcatatat ctcgactcct
     4921 tatcattttt ttgagttact tcaaaaacag catccgctta ctgcgaacca catcaccgac
     4981 ttgcatccgg tacaaataaa cacccgaggc cacaaccgaa cctgcattat tacggccatc
     5041 ccattggata gaataccttc ccggatcgcg ttgcgcattg atcaaagtag ctacttcctg
     5101 acccagcatg ttatacacct tgatcacaac tttacccgcc ttggccacat catatcgaat
     5161 cgtagtccgc ggattaaatg gattcggata attctgtgat aatgcaaact ccgtaggaat
     5221 accctcaaaa tccggattgt aaaatgcggc gatagtcaat ccacacgtag atagcaatga
     5281 aattaattta tcctgcactg atttttctaa taccaccgaa tttgtataat ccccaccttt
     5341 tgcaagcaaa attcgctcat cgccgatctg ggcatacaat ccgatagccg aattagtaaa
     5401 gttttgacgt ttttttaatc ctgaaagcgc cgttatttca tattcagtga atgccaaagc
     5461 gatttgactt ggaatactgg ttgtaagaaa tgtttttggt ttttggagcg gtatcaaatc
     5521 atccgaaccg ctgaaagatt tgtcatcatc agtcgtcaaa aaataaccat ctgatcctga
     5581 tactgttatt ttatataaat cattataaaa cgggcggccg atcgtctgca cgttaaacga
     5641 tttttcaagc gatgtcgtat tgttagattg atccttggcg gtcgctgtga tagttagctg
     5701 tcccgcttgt tggatcgtat atccgccaaa aaaaaggcta cccgactgac tcatcgtaac
     5761 cgaactatta tttatattaa caatcattga tttgagactc tcattagaag taacaacgat
     5821 tttgacatgt gtgccaaatc ccggcacaag taacccgata ttggcttctg gcgctataaa
     5881 atcaggggct gtcgcagagg ctattgcgct caatttagaa aaattacctt cactaaatgc
     5941 gccgatttta taatagtatt ttatatatcc gcctaaagtg tcccgataag tattcacatc
     6001 gccaacagta gctatacagt tacctgtcgt gtcaaaaaaa gatgaaagcc ctctatatat
     6061 cttataatag gtcgctcctg aagatttggt ccaagaaaga tcaatcacac gatccaattc
     6121 aagcgacggg ctgcacatcg gatttttcgg cggaacaaga attgtcgaat cttgaatgac
     6181 cgtaaaatca ttatttgata aaaaattgaa atgactaaaa gaaccgccga tatatatatt
     6241 ttgctcattg atatccatcc cggctatact gccgttgtat gtatgtccca tcggagaaca
     6301 tacgccggaa atcatatcaa ttttggtaaa acctgattga ggaattgccc caacactatc
     6361 aaaatcaccg gtcatatata tataatttcc cgcagtaacc atttttgtta ggcctaaacc
     6421 accgctcaat tttgcatccc aagctttaat ctccccggtc ttacgatcaa gagcagctgc
     6481 acccgaccta taaacaccct taacctgcgt aaaataacca cccaaataaa tcaccgatga
     6541 agttgcagtt atagcattta tggttttatt tgtcggtgaa tttctgatct cttgccccgt
     6601 acgggtatta aattcagata gcacgctttt aaatgaatta ccctcgaaat attgaatgcc
     6661 caacatcaga acagaatcta ctgcgcccat tgccctcagc gttaaataag acgttggata
     6721 cggaatttga agcgaagttt gccgcaattg cattgtattc aaatcaaaca aagcaatgtt
     6781 taaacgatta ttttcactaa ctgaagtaaa actgccacga atatataacg attcgccggc
     6841 tatgagtagc tgcgaaacaa tcccattcag tcccggcgcc cagttattta gctgccctga
     6901 accggtggct ccataaccaa taagagaggc cgcatacggt cgagagatat tattaacctt
     6961 attaaaataa ccacctataa gaactgtgtt ttcaaaaact tcaatactcg tcactgctcc
     7021 atctactacc ggagcccaat cgcgcaatgc accagtctgc gcatcaaaag ctgctaaacg
     7081 ataacgtgtt atatactggc taaagctatc aaaggaaccg cccgctatcg cttcattgtc
     7141 agaggaggcc gtagtaatag taaatactga accggaagga gtcttagtta tagaagataa
     7201 attgcctgta ttcaaatcga aaagcgcata attcgctttt gaggttgaac ctacttttgt
     7261 aaaaattcca cctaccaata ttttgtcgcc taaaacagaa atagcgcgaa cactactgtt
     7321 cgcatttgga aatgtattgt acgagtcaat attgggatcc catgaattga gttgatttag
     7381 cgataagcta taagccgcca accgatttct aacttgagaa aaaatctgcg taaacatccc
     7441 tccaatatag agagtcgaat ttttaacgta gagcgaaaag actatagtgt ttggccaagg
     7501 gttaaaagga tttactgctc cggatgccat atcaactgca ccacaatgct gtctttctac
     7561 tccacctaga taaaaaaatt ctcccccaat ataaacatta ttagagctga ctacaatcgc
     7621 atatacaata cccgggttat tttcactgta agtaacatgt ggcgcccaag cggtagcatt
     7681 accgctagaa atatctatag accctaagct aaaccgttct tctcctgcaa tttcagtaaa
     7741 ttgaccgcca ataataagcg tattagaaga cacaatcatg tcatatacga tcccatcagc
     7801 accgggattc caatctgaaa gtacgccatc cgctacgcct acagccgcga tatgatatct
     7861 cgtctgatca gatactttag aaaaattgcc tccgaaataa acaaaatctt catttaccgc
     7921 gatgcttcgc acttcgccgt caacgatcgg gttccaattc atatctaaag taagatctgt
     7981 ttttattctt gcaatatact tacgttttac gccgttgatg tgagtaaaat ttccagcaat
     8041 aaaccaccca tattggggat gataagcaac atcatatatt gtgcctaaaa cgcgaggaaa
     8101 atctgttttt tcctggttat tcgcatcaaa aaacatcatc gaaccggtaa ccgaaacaac
     8161 cgaattaaat tctcctccta tgtaaattgt atttccgtcc ggactggctt ttacatcatt
     8221 aacataccca tccgtaccgt aatacggatt tggattgttt tgcccttgca gcatagaagt
     8281 gaggatgata aaaagactac taaaattaat tacgatcgtt acaaagcgat gccatgaagt
     8341 tctaagattc atgatttttc ccttttttag ttaatgaaaa aattgcgtag agattaaacc
     8401 tgctatgata ttgtaaccgt caaaaaaaaa caataaaaaa agcaaggctt taaaccttgc
     8461 tttttttata gtgattggaa agaagtttat gaagctgaac caagcgattg caacttttgc
     8521 atcagtgcgg ttgctatttc aaccgatgtc ccgctcggat aggtatatgc cgcatccaat
     8581 agcttgatct tgttaaaaca agacgtataa tcacccaact gcaaataact gctcgcttgg
     8641 atccaaacta aatgactctt ggtaacgcga gtatccaaac tcatttgaaa attcgcatcc
     8701 ttacgtataa caaaatcagc acgtgaaata gcttcactcg gatcgtttaa tgcccacgac
     8761 gaaaaactcc aacccgcata ggcatccgta ttactatccg ctgctaccgt cacaaaaaaa
     8821 ctatcggcgc gggctacatt gctcaatcga accaagcacc aacccgcacc cgcgaccgcc
     8881 ggagcgccct ccgatgcaat caaattctca aaattacgca atgcggtcgt atagttcccg
     8941 gttgcaaatt gatcggtcgc tttggtcaaa gtgatcttgt ctttacctcc accaccgcca
     9001 cacgatgaaa ttgctatgga aactaagcat atcatccaaa gcttattttt tgctttcata
     9061 tatctcgact ccttatcatt tttttgagtt acttcaaaaa cagcatccgc ttactgcgaa
     9121 ccacatcacc gacttgcatc cggtacaaat aaacacccga ggccacaacc gaacctgcat
     9181 tattacggcc atcccattgg atagaatacc ttcccggatc gcgttgcgca ttgatcaaag
     9241 tagctacttc ctgacccagc atgttataca ccttgatcac aactttaccc gccttggcca
     9301 catcatatcg aatcgtagtc cgcggattaa atggattcgg ataattctgt gataatgcaa
     9361 actccgtagg aataccctca aaatccggat tgtaaaatgc ggcgatggct ttatggctaa
     9421 actcaccgct taattttccg ttggactccg cacttataag cttccattga ttgccgtctt
     9481 tttcatagag gccgatttta tatcgttgcg aagatccagg tatcattcga tccaaagtgc
     9541 tttcatttgg cagatcgtat gttattttga ggttagtcgg cgtaccggta atgccagcct
     9601 catacatgga acctatttca atccaacctg atggaatatc ggttgcctgg actaaggatg
     9661 ccacaacata tccttcaccg ctacctgtga cggaataacc gcctaaggag cttgattttg
     9721 acaagggtgt tacggtaaac tgcttattta atgttgtgct tgtattgcct acagaatcag
     9781 agcatgtcgc agttattgat actgtaccgg acgtaattga atatgatccg aagaataagg
     9841 aaccgcccaa acgactgagc gcaacagatt gcccgcccgc agtcaatgca gcagttgaca
     9901 ttccacgatt cgaactgaca gcaaacttag ctacattaat gacaggcgat gctaaagcgc
     9961 caacggtaac cgtcggggga gtttctattg catttatttt tagattatct atacaccatc
    10021 cccaacctgt agatgcagca tcagtaaaca ttctaaatcg tataaaaatt tttgtacctg
    10081 gctcgtactt atctgtcaaa tcaattcgac gttgtttaaa catagaatta gatcccgaac
    10141 ccacgctgtt aaatgtgctt aaccacgtcg agttagctct actgtcccat ccatcaataa
    10201 caggctccca actaatacca tctgaagtgg cctctacaac tacataatcc cagaattcgg
    10261 gttcaggaaa cggtactcca gggtctcccg gctctacaat agcgatttca tcaaaaacaa
    10321 tcattgatga atcgcttgca accgttatcg gataatttaa ttgaaaagta ttattcgtat
    10381 tgtttgtata cgggtgtgca gtatgcaatg caaaagagct aaaaccgctt ggcttcgtag
    10441 ctgaaaaaag actacccgta aactctccat tggctgatgt ggacgtatta aaagcattgt
    10501 aataatcatt gcgagccacg gcttgcacca aggtaaccga ttttgcaacc gtttcataca
    10561 atgtgccatt cctgtatgaa gaagctttta tgcttaccga accgatttgc gttgatgaaa
    10621 aagatactgt actgtcattt atgcctgttg accccaacgt aagagcccgg gtattaccca
    10681 taaaaacaat ggtcgagtca tatgtgtttc gcaaacctag ctcaacatta actaaaccgg
    10741 ttgcggggtt ctgataggct tgttttagtg ttggcccgag cacaacagtc ggaagcggtg
    10801 gagcgcttat gggagctgtc caaataccac gcccgtgagt tgcggcaact atttcattat
    10861 cgataatttt tagttcccat acggagacgg ccggcaaccc gttattggca taggcccatg
    10921 tcgcgccatt atccgttgaa cgaaaaatac cgatttccgt tcctgcccat atttcattgg
    10981 tatcggtcgg cataaccaat aaagagtaag tcgccacatc aggaaaacca ttgttgctac
    11041 tgctgttagc gccgaaaccg cttatatctg tccatgtttg accacgatcc attgttcgca
    11101 aaacttttgg tttatctgca atagaaaata atgcaaaagc ttcatttggg ttataaggat
    11161 gcgtcgcaat acctgttact cgtcctaacg tgacagccgt ataattgttg gttgctgtat
    11221 aggatttacc agcattggtg gaaacgtgga gtttaccagc cgcactcatg ttagttccgg
    11281 cccaaacaat attgggatcg gctaatgata tttcagccaa atttcttgcc gtggaaaagc
    11341 cataactgct tgtaatgggc gctaacgtcc aactatcacc aaaatcttcg gaacgccaaa
    11401 aaccagaacc accaggtact atcagtaggt ccggatcaag ttttgaacgc ccgatttctg
    11461 taacaaatgc gctattagca ttatcaacat ctaaaagccc gcttgttgct gaattccagg
    11521 tttgtcctcc gtcggttgtt ttgtatagac gattatcata caaacacccg ataatctgat
    11581 ttggatcatt cgctttccat acaccatcgt atccgtcacc gcctagcatc gcataccaag
    11641 atgagtcaac tgtcgcatct acaggagaca tccaagtacc attatcctgt gttccaccga
    11701 tatatgcact ggcaccgggc attttatcta caccatagaa ttgcgctgtc acgattcctt
    11761 tagcttcgtc ttcatcaaac aaccaagttt gaccgccatc ggttgaacgg gcaaaacccc
    11821 cgtcattggt atgtaaaatc caaaaattat taggctcact tattggaatc ataacaatat
    11881 tatgctgatc cgcatgtata tagggatagg tacctccgct aggacgactc aaacccggat
    11941 accaattagt cattcttgtt gttccgcgac gcccagctga gatgctgttg gaaatattta
    12001 tacgataaat attgatcccc cctacaaaca aaatgtttgg atcataggga tgaacagcta
    12061 tggtattatc ataccatcct tgtgcaccga gccatgctat actggaagcg ctttgccccg
    12121 ttgtatcagc gacagtagcc cagttggccc caccattggt tgaacgatac aaaactgagt
    12181 tggtaccgct ctcaacgcta gcatataata aatttgtatt acttggcgca atagccattt
    12241 ccaaacgatc aacagcacct atacctgtgc tgctaggtgc ccaagtgata cctgcatccg
    12301 tcgatttcaa aaaccccaac gcttctgccg ttgcatattg gatattaaaa tctgatggat
    12361 cagctaatac ctgttgtatc cgattattgc cggcagtatg tactttggtc caatttgtac
    12421 caccatctgt tgttctcata attgctgaat ttgtaccggc cactacaata tcagaattat
    12481 tgggatcaat gattaatcta ttgacgtatg caaaatcact attagatgaa gtcgcgctaa
    12541 gttgtgccca actggcgcca cgatctgttg atctaaaaat accatcgcct tttatcatgc
    12601 caactgcggc ttgataccct tcccctgttc ccgcatacat gatattgtgg tttgaagagg
    12661 ccatagcaat cactgtcgtc gaaagattag gaaattcggg agttaaattt tcccagtatg
    12721 agcctccatt tgttgttttc caaatcccac cgccaacgga tccaaggtag taagtaagat
    12781 gattcgcatc atcaggatca acaacaaggc cacgcgatct tcctaaaaaa tttgacgggc
    12841 cacgtgaagt ccaacttaat gttgaagatg acatcatttg attgcgtaca tacttctcac
    12901 cttcacgcct tataattccg gctttctcca tttcgatatt tttatagccc atttggtaat
    12961 caggtagttg cttaccatac tgggtacgaa tttcacgatg caaaatgtca aatttttcag
    13021 gttgatccgg tttagcgtaa ccttcggcac gacgtttaat tttttgttct agaagcgctt
    13081 gctttagggc ttcaggtgtg tttttaaggt gctttttttt catattagcc gttgacctct
    13141 gttcactcga accaacgatc agtattccgg ctattacaaa cgctgataga agccaaaaga
    13201 aaggcttttg taacccatag cgcatgttca ctcctcctgt tttttgttag agattagtta
    13261 gaaaagaata ttaataatga agaatcatat ttgcgacagc ctacaatttt atcctattcc
    13321 gctaaatcat gaaaagggaa ctcggctgaa atatgttgct ttaaatccta aagcggtcaa
    13381 acaaattata aaatcacacg agaacaagat aggagtcaca ctgcttctta actcacagga
    13441 aagtgaatgt gacgaatcgg attccagaaa agaatattaa ctcaataagt tacttttttt
    13501 ttcaaacata gcaagttttt tcttatacct acattcaaat gagtaagtaa taacacttat
    13561 cacgcttctt ttttgcgatt tccatacata ttttgactta gagaatacta agttcgatcc
    13621 tgtcggaatc ctgtagttgc aaattggtta ctttcaattc tgcatttctc ccagttctat
    13681 acactgcttt acccaaacaa tcatacttgg aaatacaatc attcattttg gaaatccaac
    13741 caacctttat cgtaaaaccg ttcctaaaat ataaaaatta aattcgtgcc tgtaagttac
    13801 ccaacaaaat acatatgccc ggactaagcc gggcatatgt attattcaag tattcgatta
    13861 tgttgaaaat tttatactga aaaaatcagc atttacttct gccaaaaact cttccttaaa
    13921 atcattttta aatattctta ctgtttcttt tccgctccgc tcgggcaaca gctatgtgtt
    13981 tccgttgaag cggttgcttg ttgttcagaa gattttactt cctgcgcttt tacttctgtt
    14041 gatttgcagc aatctccgcc cgtcataccc ggaggacaag tttcggatgt attcatacga
    14101 tacgcataga cagcaccgac agcgataaca gctatggccg caatcgtgat gatcattttt
    14161 ttattcatac gatctcctta caaggttaag agtaaatatt ggttatgatt taaaatttta
    14221 tatcaatttt aattccgcca aacatgtttt gattcgcgcg aacgaacgcc ctatatcgct
    14281 gtctaatccg acggacatac gcaccaaccc atcaccaaga cccatcgcct gacgctcttc
    14341 ttcaggaatt tccgaagacg tactatgccc gggtgcactg aatagtgttt tgaagtatcc
    14401 tagactcacg gcaaggtaac cgactttttc ttgttgcatc cgcgtcatca gcgcctcggc
    14461 tttttccgat gtccctgcat caatcgccac cattccgcca aatccaaact gtgcattcat
    14521 catagacttc agaagtgtgt gttggcgatg ggacaccaat cccggataat agacacgtaa
    14581 gcccaatttt tcaagttcgg atgcaatata tagcgcgttg cggctgtgct gttggatacg
    14641 aatatggagg ctatgc
//
LOCUS       DAOQGF010000087        14513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1909_length_14513_cov_6.322659, whole genome shotgun sequence.
ACCESSION   DAOQGF010000087 DAOQGF010000000
VERSION     DAOQGF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1909_length_14513_cov_6.322659"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK937_11520"
     CDS             complement(<1..514)
                     /locus_tag="PK937_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH33243.1"
                     /translation="MKNKIHGFVVILLCGLFWIGCKGNERATTPTTGALPLICDDAVS
                     NVIQIQKNEFEKRYTQAKIDMQLAPASAAIVKLINNEVECIISSRELDSTETDFLIRH
                     EIKVFSQKFALDGIAIIVNSENPIEEMSLASLRELFSGDATYWSDITDSVRFPYHLNR
                     IQIVSDGRRSG"
     gene            complement(504..737)
                     /locus_tag="PK937_11525"
     CDS             complement(504..737)
                     /locus_tag="PK937_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33244.1"
                     /translation="MIGEWDINRKIRFGVALLFVATGIWVMIGNVMPDYINDRYHYVM
                     GFILIFYGVFRFVHYDYRKNIKRTDEISEENEE"
     gene            complement(739..2322)
                     /locus_tag="PK937_11530"
     CDS             complement(739..2322)
                     /locus_tag="PK937_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH33245.1"
                     /translation="MSDAKKSKTVLTDAGIEVPLYLKEYPQDYSTKLGDPGQFPYTRG
                     VQNTMYRGRLWTMRQYAGFGNAEESNKRYKMLLAQGTMGLSVAFDLPTQIGYDCDHAL
                     AEGEVGKVGVSISSLEDMEVLLDGIPLDQISTSMTINATAAILLCLYVAVAKKRGVDI
                     RQLSGTIQNDVLKEYIARGTYIYPPKHSMRIITDIFRYCTSELPKWNMISISGYHIRE
                     AGSTAVQELAFTFANAIAYVEAAVASGQDVDQFASRLSFFFNAHNNFFEEIGKFRAAR
                     RIWAHIIKERFGANDPKSMMLRFHTQTGGSTLTAQQPYNNIPRVTLQALSAVLGGTQS
                     LHTNSFDEALALPTEDSARIALRTQQVIAYESGVAQSVDPLAGSYLVEQMTDEIETRV
                     MRYLKDIDAMGGSIRAIESGYFQSEIAEAAYRYQKAIETKAQIIVGVNDFKVENDSKP
                     DILQIDTSIEKNQKERIQKLRARRDNAKVQSGLEKLAQACRGTDNILPCILESVEAYA
                     TLGEIADTMRNVFGEYKAA"
     gene            complement(2319..2966)
                     /locus_tag="PK937_11535"
     CDS             complement(2319..2966)
                     /locus_tag="PK937_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNH33246.1"
                     /translation="MQEPWRLLRIMAEFVDGFEKMERVGPAVSIFGSARTKPGMANYK
                     LAEELARLIVKSGFAVITGGGPGLMEAANKGAKEAGGKSVGLSIQLPFETKGNDFLTM
                     EVNFNYFFVRKVMFVKYARGFIALPGGLGTMDELFEMLTLIQTGKVDPFPIVLIGKTY
                     WSGLLDWMKASMLKERYINPEDLELFHVTDSAKEAVRIIQDFYKKNKKRGKIRII"
     gene            complement(3043..4041)
                     /locus_tag="PK937_11540"
     CDS             complement(3043..4041)
                     /locus_tag="PK937_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33247.1"
                     /translation="MNVALSQTPTVLIVDDSEDMILLLTEVLEDAEYRVVTARNGHEA
                     LDMVVQANPDLILLDAIMPNMNGYEVTQILKTQDKTRLIPIIMLTGLSDLNDKLKGIE
                     LGVDDFLVKPFNHLELVTRVRSLIRVKQYTDELENAETVIFSLALAVEAKDEYTEGHC
                     NRLSFYGARLAEAIGLSTEQIKAVRRGGILHDIGKIAIHDNLLLKPGPLTPDEFEVMK
                     QHTVIGERICKPLKSLNGVLPIIRSHQERWDGSGYPDGLKGEEIPIIARVIMTVDLYD
                     ALTTDRPYRRALSNEKAFEVMREETAMGLWDAMLMEKFIDMMQKNEFGTPTGRSAL"
     gene            complement(4054..4506)
                     /gene="queF"
                     /locus_tag="PK937_11545"
     CDS             complement(4054..4506)
                     /gene="queF"
                     /locus_tag="PK937_11545"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNH33248.1"
                     /translation="MKKKIHHKHVTDAKAFVQKPDFKFKFEGYEAIRPELIETFAYEN
                     PGQPQAIVVSTEEFTAVCPWSGLPDFARIVVTYVPRQKIIELRSYKYYLYSYRNVGIF
                     QEHLTPRLLQDLVAACEPESMTVKTDYNIRGGIHTVCEASYRRNTKRR"
     gene            complement(4517..5218)
                     /locus_tag="PK937_11550"
     CDS             complement(4517..5218)
                     /locus_tag="PK937_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNH33249.1"
                     /translation="MRKLVLWDIDGTLIRCGKSPRNAIVAAMEEVFGTRGDVDNYPFS
                     GKTDPQIIYDVMTAAQMDEQHVLANMHVALAGYVARLEKTLMPDEIRIFEGIVDLLNQ
                     LSGHNEVIQGLLTGNVIEGARIKLSRAGLGHYFFNGKPPLGAFGSDARHRPELPAIAV
                     QRAWEMTGRRFESKDIVIIGDSPYDVLCGKHLNVRSIAVATGWHSVEDLKPYEPDAVY
                     TDFSDTSDVLKTILA"
     gene            complement(5222..6913)
                     /locus_tag="PK937_11555"
     CDS             complement(5222..6913)
                     /locus_tag="PK937_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH33250.1"
                     /translation="MKFASKLFLVFVGFSAFLSLAFVGTFYLFVFKKAELDLKSQAQL
                     HAYSLAVTLSKLIDGDKHQTILTRADENSEAYKEIQRVLRTFRDANREVGMPVQYVYT
                     KIRTEHKDTLAYVVDAQEDDRSIASIDPTTKDTTFNFTPVGQRVEVTWQREFQKASVS
                     DFYSDPWGMWITGAGPIRNSAGTVVAFAYVDVSGTKVLEELNAIKQRITVVSVIALIA
                     LLTLSAILSYVLANFVNRPLNNLREGLTRIKDGVFDFRIPINRKDDFQDLAEAFNVMS
                     ENMRTMAAQISNGSRKIASTSTEILAISREQATTSSQQSISVTETTATMEELTSTSRY
                     IAENSESVVKIAAETHEISQEAVDLSGVAKDKMEEIRRKSDQDTSAIADLNKKMQKIT
                     EVIEIINTVTEQTKLISFNARLEATGAGEAGRRFSVVASEIRRLAENVAESTEEIKET
                     VHEIRVAMDSLVKNSKESAYKIREGVDLVSKVSTVLENILLAAQNTTESAKQISLSTQ
                     QQRTASEQVVTTLKEISEGAKHFVKSSNQAAIISGDLNALSDELNKTLNNFKVGR"
     gene            complement(6924..8078)
                     /locus_tag="PK937_11560"
     CDS             complement(6924..8078)
                     /locus_tag="PK937_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH33251.1"
                     /translation="MLHRYSVLTKLFLTLIGMLSLLLTAGFVVVSSGMALGWALLWCV
                     MAFALAAIIGYLLIRRITEPLYLMIGTLDSRSDFLSLSIPQVEQRRDEWGELYQHLHA
                     RLMLWKNLRTNMDERLHVMAKPLTDGDHAIRMQHDAMAEVQHALAEQITRHETNLRSS
                     VAAAKTYQELRIKLSDMAEAQKKIMRQAHTVYQQLFSSREQQSSRIERLGQLIKKFDD
                     VSDVVRRVHFINDQTKLIAFNAAIEASSNGEHGRRFAIIASDIRNLSQTIDDSNIDMK
                     NILSDLQTALSSLIESARADSHRVMESVQTLNEAVIWMERNQNEMNDALETAATISKM
                     FSDNHAAVMAHTENIKRIQHSIQTTNAVFNSLKNHIHDLKAGYQSIGSLS"
     gene            complement(8068..8487)
                     /locus_tag="PK937_11565"
     CDS             complement(8068..8487)
                     /locus_tag="PK937_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33252.1"
                     /translation="MKAGQHANTMDVLVFEVGTTLYGIPIGQIDMLDFVKSESQIPAG
                     NQIRHLLGFVSDRNPISQRSVKIKNPQPLRFIVEGIYGVFTLDVRRIADLPKTIGHQV
                     ERSPVSSAYISPKEQWDGRLVLLLDLQKKHITERYAA"
     gene            complement(8484..8999)
                     /locus_tag="PK937_11570"
     CDS             complement(8484..8999)
                     /locus_tag="PK937_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH33253.1"
                     /translation="MDLKKTDNAELKLRSLGNTEEIAIDEEAIQYVVVTCGKTHFAFE
                     GIYVKEILPQTDITFVPGLPNFFSGMIHVRGNLECVADLGQLIGLQPTKTDKLNRVLI
                     VRYEEISSGILTEYVLDLIDVPKSKIRSDIKHTDAGRSDYFIGEFEWNNQIVVILDIV
                     RLMRKINGEEL"
     gene            complement(8977..9480)
                     /locus_tag="PK937_11575"
     CDS             complement(8977..9480)
                     /locus_tag="PK937_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33254.1"
                     /translation="MRSLYAKVLILGYGFWFWACGSSQPVPADAPFLHGLEVARWAET
                     ASVIRERVHSVAGWQWVTVLRDQNGLETTIVVRHVDEEYHLELDLNGRMQSISRIYPI
                     AQLDSAVSQLERMYREPDKVMQRPTYQMRRWEVYPEGYGVWIEMMITKEFFSIQAVYK
                     NGSQKNG"
     gene            complement(9483..10892)
                     /locus_tag="PK937_11580"
     CDS             complement(9483..10892)
                     /locus_tag="PK937_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH33255.1"
                     /translation="MEELIKKIGAVADAEQIEAFVVGGYVRDRILGRKVTDFDVMVIG
                     DGVRFAEHVAKAFRLQTLVVYENFGTAMLPMGEKYDHLKVEFVGARKESYRHDSRKPL
                     VEVGDLQSDLSRRDFTINALAMRINAQDYGVILDLFNGRADLDKKTLRTPLDPDETFS
                     DDPLRMMRAVRFASQLQFTIDPITYDGICRNAQRIAIVSQERITDEFLKILGSPKPSV
                     GLYLLESTGLLAIIFPELTWLSGVEQQQGYLHKDVFKHTLKVVDNIGEMTNDLRLRFA
                     ALVHDIGKPRTKRFVEGIGWTFHGHEDVGSRMARGIGKKLKLPNDFWLYVSKLVKLHM
                     RPIQLVDEGVTDSAIRRLLFDSGADFDDLLTLCRADITSGNKERAKRHLENFQRVLDR
                     ARDVEEKDRLRAFQPPIRGDEIMQMFGLPPGPTVGALKKIIEEAILEGIIPNEYQAAY
                     DHLMAHKDEVLQSAENMQP"
     gene            complement(10893..11174)
                     /locus_tag="PK937_11585"
     CDS             complement(10893..11174)
                     /locus_tag="PK937_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33256.1"
                     /translation="MKTAFRQHVEQTVGARGIYSEREKRQLIEHVTQIHDALLAGEKT
                     DTIMENVEAFLDQFVNAFSTLEQKTDARNKKVQRREKKKSQLDIFKNEK"
     gene            complement(11171..12193)
                     /locus_tag="PK937_11590"
     CDS             complement(11171..12193)
                     /locus_tag="PK937_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH33257.1"
                     /translation="MTAPQVNFSELQQLTDTDAIARLRQSRSAIEAEVAKAIVGQRAI
                     VEQLTIALFARGHCLLVGIPGLAKTLLINTIARALDLKFNRIQFTPDLMPSDIVGTEV
                     IEENMTTGSKQFRFVRGPIFANIVLADEINRTPPKTQSALLEAMQEHKVTASGNTYHL
                     EEPFFVLATQNPIEQEGTYPLPEAQLDRFMFNIWVDYPSADEEVAIVKNTTSSYHYAL
                     EKVLDAESILFYQNLIRRVPVADNVVRYAVDLVSKTRPKTPGAPKFISDWLSWGAGPR
                     ASQYLILGAKTRAALDGRFTPDIDDVRAVVKPVLRHRLITNFTAEADGVTTLDIIDKL
                     VAETTQ"
     gene            complement(12190..12579)
                     /locus_tag="PK937_11595"
     CDS             complement(12190..12579)
                     /locus_tag="PK937_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33258.1"
                     /translation="MFRTQGQYYWTDALYLARSKDQLRPVWREEKNTLYQCVHCAAFV
                     RFTEEQTGQWVRLKPYEQFQRRKEFLSFAPDAKQMKLIRQETRDRALFRCPVCASHWW
                     LEGDEWTHADDAIVEWAENSEFSKGTT"
     gene            complement(12749..14461)
                     /locus_tag="PK937_11600"
     CDS             complement(12749..14461)
                     /locus_tag="PK937_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNH33259.1"
                     /translation="MIRSRFFHLLALFLLTQSTGWTQQKLNTAISDVTVYTDRAAITR
                     SGEIYLNPGEYQFVVSDLPHALQDQSLRVGGSGTAMARISDIKIELDYIDTMPQNRLK
                     DLQDQLALLMQDERVFNDRLGLLGKEKELLDQIKNSITQNKESNKLSFDDWSKLFGFY
                     DANYNKLNDEIRMLEKKRGDLTVRKNALQNQINQLSGYGKLVKKKVFLTVNVSKPGMM
                     NFAIDYVIGGAKWYPVYDVRVSPEDKSVDMTYYAMIAQRTGEDWSNVNLAISTARPNV
                     SGSMPQLSAWNLSVYQPIGILGRGGRSGAGMGAMSSAKMTKSAEEISDMSASSGAGEV
                     PEEAEAPIYEMETDEAAIETKSTSVVFKIKRKTNIPSDNYDHKVTITSESLKSDFAYS
                     SVPKLAELVYLRASIENTTEAPFLAGNANVFFGSNFVGTTYLNTVIPTEKFDVSLGID
                     DGIRIKRQQIRDYNAEKGIFNKSVKKTFEYKITIESFKRTDDTILVVDQFPQSQDERI
                     KVEAVLPEFPKEKWSSPHPNGVIEKTGGGLVQWRLRIKPKEKIELRIKYVVEYPRDVK
                     VDGL"
BASE COUNT         3730 a         3630 c         3111 g         4042 t
ORIGIN      
        1 taccggagcg acgaccatcg ctgacgattt gaattcggtt gagatgatac ggaaatcgca
       61 ccgagtctgt gatatccgac cagtacgtcg catctccgga aaaaagttct cgcagcgaag
      121 caaggctcat ttcttcgatg ggattttccg aattgacaat aatggctata ccatcgagcg
      181 caaacttctg agaaaaaacc ttaatttcgt gacgaattag aaaatccgtt tccgtcgaat
      241 ccaattcacg cgaactaatg atacactcca cttcgttatt gatcagcttg acgatcgccg
      301 ccgaggccgg cgctagctgc atatcaattt tagcttgtgt gtaccgtttt tcaaattcat
      361 ttttttgaat ctgaataaca ttagaaacag catcatcgca gatcaaaggt aaagcacctg
      421 tcgtaggtgt tgttgctctt tcgttaccct tacaaccgat ccaaaataaa ccgcataata
      481 agataacgac aaatccgtgt attttattct tcattttctt ccgaaatttc atcggtacgt
      541 ttgatgtttt ttctataatc ataatgcacg aagcgaaata cgccataaaa tataagtata
      601 aagcccatta cgtaatgata acgatcattg atataatccg gcataacatt accgatcatc
      661 acccaaatac cggttgcgac aaaaagcaag gctacgccaa aacgaatttt gcgatttata
      721 tcccattccc cgatcatttc aagcggcctt atattctcca aaaacgttgc gcatcgtatc
      781 ggcgatttcc cccaaagtgg cataggcttc taccgattct aatatacacg gcaggatatt
      841 gtccgtaccc cggcaagcct gagctaattt ttctaacccg gattgaactt tagcattatc
      901 acgtcgcgca cgaagttttt gaatacgttc tttttgattt ttttcgattg atgtatcaat
      961 ctgaagtatg tccggcttcg aatcgttttc gactttgaaa tcattgacgc cgacgataat
     1021 ctgcgccttg gtttcgatcg ccttttgata acggtatgcg gcttcagcga tttcactttg
     1081 aaaataaccg gactcaatcg cacgaatgga accgcccatc gcatcaatgt ctttcagata
     1141 gcgcatgact cgcgtttcga tctcatccgt catttgttcg accagataac ttccggcaag
     1201 cggatcaact gactgtgcaa cgccgctttc atacgcaatc acctgctgtg tgcgaagggc
     1261 aattcgcgcc gaatcctctg tcggtaatgc cagcgcttca tcaaacgaat tagtatgtaa
     1321 actttgcgta cctcccaaaa cagccgataa cgcttgtaac gtcacgcgtg gtatgttatt
     1381 gtatggctgc tgggctgtca gcgtagaacc gccggtctgc gtgtggaaac gcagcatcat
     1441 gcttttgggg tcattcgcac caaatcgttc tttgatgata tgtgcccaaa tccttcgtgc
     1501 cgcacgaaac ttgccgatct cttcaaaaaa attattatgt gcattaaaaa agaaactcag
     1561 tcgcgaggca aactgatcca catcctgacc ggaagcaacg gccgcttcaa cataagcgat
     1621 ggcattggca aatgtaaatg ccaactcctg cacagccgtg gagccggctt cgcggatatg
     1681 atacccggat atcgaaatca tattccattt gggcaactcg ctcgtacaat agcggaaaat
     1741 atcagttata atccgcatgg aatgtttggg tggataaatg tacgtaccgc gtgcaatata
     1801 ttcttttaga acatcgtttt gtatcgtacc ggaaagctgg cgaatatcta cgccgcgttt
     1861 ttttgctacg gcaacgtaca ggcataataa aatcgccgct gtcgcattga tcgtcatgga
     1921 tgtgctgatc tgatccagcg gaatgccatc caaaagcact tccatgtctt cgagagatga
     1981 aatcgaaaca ccgaccttgc ctacttcgcc ttcagccagt gcgtggtcgc aatcgtaacc
     2041 gatctgtgtg ggcaaatcaa atgctacgga caaccccatc gtaccttgcg ccaaaagcat
     2101 tttgtagcgt ttgttggatt cttctgcgtt tccaaatccg gcatactgac gcatggtcca
     2161 cagacggccg cgatacatcg tattctgaac accgcgtgta tagggaaact gtcccggatc
     2221 gcccagcttc gtcgaataat cctgcgggta ttccttgaga tacaagggca cttcgatacc
     2281 cgcatcggtc agcacggttt tacttttttt tgcgtcgctc atattattct gatttttccg
     2341 cgttttttat ttttcttata aaaatcctgt atgatgcgca ccgcttcttt agccgaatcg
     2401 gttacatgaa acagttccaa atcttcgggg ttgatgtatc gttctttgag catggatgct
     2461 ttcatccaat caagcaaacc gctccagtag gttttaccga taagcacgat cggaaaggga
     2521 tccactttac cggtctgtat taacgtcaac atttcaaata attcgtccat cgtacccaga
     2581 ccgccgggca aagcaataaa accgcgggcg tatttgacga acatgacctt gcgcacaaag
     2641 aaataattga agtttacttc catcgtcaga aaatcattgc ctttggtttc aaagggcagt
     2701 tggattgaga gcccaacgga cttcccgcct gcttctttgg cgcctttatt agccgcttcc
     2761 ataagccctg ggccgcctcc ggtgatcacg gcaaaacctg atttgacgat taggcgcgct
     2821 aattcttctg ccaatttata attagccatg cccggttttg tgcgggcgga accgaatatc
     2881 gaaacagccg gtcccacgcg ttccattttt tcaaagccat ccacaaactc ggccatgata
     2941 cgcaaaaggc gccacggctc ttgcatgcga taggacttat ctacggtttc taaattatca
     3001 tgctgattgc ggtgattctt aggcaactgt tttctcctgc atttataagg cgctgcggcc
     3061 ggtcggtgta ccgaattcgt tcttctgcat catatcaata aatttttcca tcaacatggc
     3121 atcccataaa cccatcgctg tttcttcgcg cataacctca aaggcttttt cattggataa
     3181 agcccgacgg taaggacggt ctgtagtcaa tgcgtcgtat aaatccacgg tcatgatcac
     3241 acgggcgatg ataggtattt cttccccttt aagcccgtcc ggatatccgc ttccgtccca
     3301 tcgttcctga tgactgcgaa taataggtag tacgccgttg agcgatttga gaggcttaca
     3361 aattcgctcc ccgatcaccg tatgttgctt catcacttca aattcgtccg gcgtcaacgg
     3421 accgggctta agaagtaaat tatcatgaat ggctatctta ccgatatcat gcaaaatgcc
     3481 gccgcgacga acggccttga tctgctcggt agataaacca atcgcttccg caagcctggc
     3541 gccataaaac gataatcggt tacagtgccc ttcggtatat tcgtctttgg cttcgaccgc
     3601 cagcgccaaa ctaaaaatta cggtctcggc gttttcgagt tcgtccgtat attgttttac
     3661 ccggatcaag gaacgaacgc gcgtcaccag ttctaaatgg ttaaacggtt tgacaagaaa
     3721 atcatcaaca ccgagttcga tccctttgag tttgtcatta aggtcagaca aaccggttaa
     3781 catgatgatc gggataaggc gtgttttgtc ctgtgttttg aggatctgtg tgacttcata
     3841 accgttcata ttaggcatga tcgcatcaag caatatcaaa tccggattgg cctgtacgac
     3901 catatccaac gcttcgtgtc cattgcgtgc agttacgacc cggtattcgg catcttccaa
     3961 aacctccgtc agcagaagga tcatatcctc gctgtcatcg acgatcaaaa cagtaggtgt
     4021 ttgcgacaaa gcaacattca taagtacaac cttttaacgg cgtttggtgt tccgacgata
     4081 tgaggcttca cataccgtgt gaatgcctcc ccgaatatta taatccgttt taactgtcat
     4141 gctttcaggt tcgcatgcgg ccacaagatc ctggagtaat cgcggtgtca aatgttcctg
     4201 aaatataccc acattgcgat aggaatacag gtaatatttg taggaacgca attcgatgat
     4261 tttttggcgc ggcacatacg tcacaacgat ccgcgcaaaa tccggaagac ccgaccaggg
     4321 acatacggcc gtaaattctt ccgttgaaac cacgatcgcc tgaggttggc cgggattttc
     4381 atacgcaaat gtttctatca attcaggacg aatcgcctca taaccttcaa acttaaattt
     4441 gaaatcgggc ttttgtacaa acgctttggc atcggtaacg tgtttatgat gtatcttttt
     4501 tttcactggt gcctcttcaa gcaagtatgg tttttaaaac atcggatgta tcagaaaaat
     4561 cggtatagac tgcatccggc tcatagggtt tcaaatcttc aaccgaatgc catcccgtag
     4621 ctacggcgat cgaacggaca ttgaggtgtt ttccgcacag cacatcatac gggctgtcgc
     4681 cgataatgac aatatctttg gattcaaatc gccggccggt catctcccag gctcgttgaa
     4741 cggcgatcgc gggaagttca ggacgatgtc gtgcatcgct gccaaatgct ccaagcggag
     4801 gtttaccgtt aaaaaaataa tgccccaacc cggctcggct cagtttgatg cgcgcacctt
     4861 cgattacatt accggtcaat agaccttgaa tgacttcatt atgtccagat agttgattca
     4921 gaagatcaac aatgccctca aaaatacgaa tttcatcagg catcagtgtt ttttcgagac
     4981 gcgcaacata acctgcgagg gcaacgtgca tgtttgcgag cacatgctgt tcatccatct
     5041 gagcggccgt catcacgtca tatatgatct gaggatcggt ttttcctgaa aaaggataat
     5101 tatccacatc accgcgcgtt ccgaaaactt cttccatcgc cgctacgatg gcattacgcg
     5161 gcgatttacc gcagcgaatc aatgtaccgt caatgtccca taataccagt ttgcgcatcg
     5221 tttaacggcc tactttgaaa ttattgagcg ttttattgag ttcatccgag agcgcattga
     5281 gatcgccgga tatgatggcg gcctgattgc ttgatttgac aaaatgtttt gcgccttcgg
     5341 agatttcttt taatgtagtg accacctgtt cgctcgccgt acgttgctgt tgcgtggaca
     5401 gggaaatttg tttggccgat tcggtagtgt tttgtgccgc tagaaggatg ttttccagta
     5461 cggtggagac tttggatacc agatccacac cttcacggat tttgtatgcg ctttctttgg
     5521 agtttttaac caacgaatcc atagccacac gaatttcatg tacggtttct tttatttctt
     5581 cggtggattc ggcaacgttt tcggccagac ggcgaatttc cgacgccacg acggaaaacc
     5641 gccgtcccgc ttcgccagcc ccggttgctt ccaggcgcgc attaaacgaa ataagtttgg
     5701 tctgttcggt gaccgtattg atgatctcaa taacttcggt gattttctgc atttttttat
     5761 tgagatcggc aatcgcgctg gtgtcctgat cgctcttacg gcgaatttct tccattttat
     5821 cttttgccac accggaaaga tcaacggctt cctgagaaat ttcgtgcgtt tccgccgcga
     5881 tttttactac ggattccgaa ttctccgcga tataacgcga tgtcgaagtg agttcctcca
     5941 tcgtcgcggt ggtttcggtt acggaaatag attgctgact ggacgtcgtc gcttgctcgc
     6001 gtgaaatggc aagtatttct gtggacgtcg aagctatttt acgtgaaccg ttggatatct
     6061 gagcggccat cgtacgcata ttttcagaca tcacgttaaa ggcttccgcc aaatcctgaa
     6121 aatcatcttt gcgattgatc ggtattcgaa aatcaaacac gccatctttg atgcgcgtaa
     6181 gtccctcccg cagattgttt aacgggcgat tcacaaaatt agccaatacg taactcaaaa
     6241 tcgccgaaag cgtcaacagc gctatcaatg cgataacaga aaccacggtg atgcgttgct
     6301 tgatcgcatt gagttcctcc aaaactttcg taccgctgac gtccacatag gcaaaagcaa
     6361 caaccgtacc ggcgctgttt cgaataggcc ccgcccctgt aatccacatc ccccagggat
     6421 cggagtaaaa atcgcttacg gaagcttttt gaaattcgcg ctgccacgtc acttcgacac
     6481 gctgccctac gggcgtaaaa ttgaacgtcg tatccttggt cgtcggatca atgctggcta
     6541 tggaacgatc gtcttcctgt gcatctacga catacgccaa agtatctttg tgttccgtgc
     6601 gaattttggt atacacatat tgtaccggca taccgacttc tctattggca tcgcgaaatg
     6661 tccgcagtac acgctgtatt tccttatagg cttccgaatt ttcgtccgct cgggtaagta
     6721 tggtttggtg cttatccccg tcaataagct tgctcaacgt tacggccagt gaatacgcat
     6781 gcaactgcgc ttgcgatttc aggtccagtt cggctttctt aaaaacaaat aaatagaacg
     6841 ttcctacaaa tgccaggctc agaaaagccg aaaacccgac aaataccaga aaaagtttgg
     6901 atgcaaattt catacaagct ccgtcagctt aaggatccga tggattgata accggccttg
     6961 agatcgtgta tatgattttt taaagaatta aatacggcgt ttgtggtctg gatagaatgc
     7021 tgaatgcgtt tgatattttc cgtgtgcgcc ataaccgcag catgattatc ggagaacatt
     7081 ttagaaatcg tcgcagccgt ctcgagagca tcgttcatct cgttttgatt acgctccatc
     7141 catatcacgg cttcattcag tgtttggacc gattccatga cgcggtgcga atctgcacgc
     7201 gccgattcga tcaacgagga caatgccgtt tgcaaatcac tcaaaatatt tttcatgtct
     7261 atatttgagt catcaatggt ctgcgagagg ttacgaatat cggatgcaat tatggcaaaa
     7321 cgccgcccgt gttcaccgtt agatgatgct tcgatggccg cattaaatgc gataagttta
     7381 gtttgatcat tgataaaatg aacgcggcgc accacgtcgc tgacatcatc aaattttttg
     7441 atcaactgac caaggcgttc tatgcgactg ctttgctgtt cgcgcgagga aaacaattgt
     7501 tgatacaccg tgtgggcttg acgcatgatc tttttctgcg cttcggccat gtccgaaagt
     7561 ttgatacgta attcttggta cgttttggca gcggcgacgg atgaccgcag attggtttca
     7621 tggcgggtta tttgttccgc caaagcgtgt tgcacctctg ccatcgcgtc atgttgcatc
     7681 cggattgcat gatcaccgtc cgtaagaggt ttagccatga catgcagccg ttcatccata
     7741 ttcgttcgca ggtttttcca caacatcaga cgcgcatgca aatgttgata caattccccc
     7801 cattcatcgc gacgttgttc gacctggggt attgataaag acaggaaatc cgaacgtgag
     7861 tccagcgtac caatcattaa atagagcggc tcagtaatac gtctgatcaa caaatatccg
     7921 atgatcgcgg ccaaggcaaa cgccatgaca caccacagca gcgcccagcc caaagccatg
     7981 ccgctgctca ctacgacaaa tccggccgta agcaacaaac tcaacatgcc gatcaaggtc
     8041 aaaaataatt ttgtaagtac actataccta tgcagcataa cgttccgtaa tatgtttttt
     8101 ttgcagatca agcaataaaa ctaaccggcc gtcccattgc tctttgggcg atatataggc
     8161 cgatgaaacc ggggatcgct ccacctgatg cccgatcgtt ttgggtaaat cggcgatacg
     8221 acgcacatcc aatgtaaaaa ctccgtaaat accttcgacg ataaatcgca atggctgcgg
     8281 gttttttatt tttaccgacc gttgcgaaat cggattcctg tcggacacaa accccaacaa
     8341 atgacgaatt tgatttccag ccggtatctg cgattccgat tttacaaaat caagcatatc
     8401 aatttgcccg atcggaatac cgtacagcgt cgtaccgacc tcgaaaacca atacatccat
     8461 agtgttggcg tgttgaccgg ctttcatagc tcctcaccgt ttatttttcg catcagccgg
     8521 acaatatcga gtatcacgac gatctggtta ttccactcaa attcgccgat aaaataatcg
     8581 gatcgcccgg catcggtatg cttgatatcg gatcgaattt tgctttttgg cacatctatc
     8641 agatccagaa catattccgt caaaataccg gatgaaatct cttcataacg tacaatcaga
     8701 acacgattca gtttatcggt ttttgtcggc tgaaggccga tcaattggcc tagatccgca
     8761 acacattcca gatttccccg cacatggatc atgccggaaa aaaaattggg caatccggga
     8821 acaaacgtaa tgtccgtttg cggtaaaatt tccttcacgt aaataccctc aaatgcaaaa
     8881 tgcgtttttc cgcatgtaac gaccacatac tggatcgctt cctcatcgat cgctatttct
     8941 tcggtatttc cgagactgcg taatttgagt tcggcattat ccgttttttt gagatccatt
     9001 tttatatacc gcttgtattg aaaaaaactc tttggttatc atcatttcga tccatacgcc
     9061 gtagccttcc ggatacactt cccatcgccg catctgatac gtcggacgct gcattacctt
     9121 gtcaggctca cggtacatcc gctccagttg cgaaaccgca ctgtcaagct gagctatcgg
     9181 ataaatacgg cttatgctct gcatccgtcc attcaaatcc aactccagat gatactcctc
     9241 atcaacatgt cggacaacga tggtcgtttc aagtccattt tgatcacgaa gcaccgtcac
     9301 ccactgccat cctgctacag aatgcacacg ttcacgaata actgatgccg tttctgccca
     9361 tcgggcaacc tccagtccgt gaaggaatgg cgcatcggcc ggaaccggtt gggatgaacc
     9421 gcaagcccaa aaccaaaaac cataaccgag aataagaacc ttagcgtata acgatcgcat
     9481 acttacggct gcatattttc tgctgattga agcacctcat ctttgtgcgc cataaggtga
     9541 tcgtacgccg cttgatattc gttaggtata ataccttcga gtatcgcttc ttctattatt
     9601 tttttcaaag cacccactgt cggtcccggt ggcaaaccga acatctgcat gatctcatcc
     9661 ccgcgaatcg gcggctggaa tgcacgcaga cgatcctttt cttccacatc gcgcgcccga
     9721 tctaatacgc gctgaaaatt ttccaagtga cgtttggcac gttctttatt tccggatgtg
     9781 atatccgcac ggcataacgt caacaaatcg tcaaagtcgg caccgctgtc aaaaagcaac
     9841 cgccgaatcg cactatccgt aacgccttca tccacaagct gaataggacg catgtggagt
     9901 ttgactaact tagaaacgta caaccaaaaa tcgttgggta gcttgagctt ttttccgata
     9961 ccccgcgcca tgcgtgaacc cacgtcttca tggccgtgaa acgtccatcc gataccttcg
    10021 acaaaccgct tggtacgcgg cttgccgata tcatgcacca acgcggcaaa tcgcaaccgt
    10081 aagtcatttg tcatttcgcc aatattatca acaactttga gcgtatgttt gaaaacatct
    10141 ttatgaagat acccttgttg ctgctccaca ccggaaagcc atgtcaattc cggaaaaata
    10201 atcgccagta aacccgttga ttccagcaga tacaatccca cagacggttt agggctgccg
    10261 agtattttga gaaactcatc ggtgatccgc tcttgactga cgattgcgat acgctgcgca
    10321 ttcctgcaaa tcccgtcata cgttatcgga tctatggtaa actgcagttg cgatgcaaac
    10381 cgcaccgcac gcatcatgcg cagcgggtcg tcggaaaaag tctcatccgg atcaagcggc
    10441 gtacgcaggg tttttttatc caaatccgca cgaccgttaa aaagatccaa gatgacgcca
    10501 taatcttgcg cattgatacg catggccaat gcgttgatcg taaaatcgcg ccgtgacaaa
    10561 tccgattgca aatcgcccac ttcgacgagc ggtttgcgcg aatcatgacg gtaactttct
    10621 ttacgcgcac cgacaaattc gaccttcaaa tgatcgtatt tttcgcccat cggcaacatc
    10681 gccgtaccaa aattttcata cacgaccaat gtttgcagtc gaaatgcttt ggctacgtgt
    10741 tcggcaaaac gaacaccgtc cccgatgacc atgacgtcaa aatccgtaac tttacggccc
    10801 agtatccggt cacgaacata gccgcctact acaaaggctt ctatttgttc ggcatccgca
    10861 acggcaccta ttttttttat gagttcttcc acttattttt cgtttttaaa aatatccaac
    10921 tgcgattttt tcttttcgcg gcgttgtact tttttattcc gtgcgtccgt tttttgttcc
    10981 aatgtcgaaa aggcattcac aaactgatca agaaatgctt cgacgttttc catgatggta
    11041 tcggtttttt caccggccaa taacgcatca tgtatctgcg tgacgtgttc gatgagctga
    11101 cgcttttctc tttcggaata gataccgcgt gccccgacgg tctgttcgac atgttggcga
    11161 aaggctgttt tcattgcgtg gtttccgcaa caagtttatc aataatatcg agcgtcgtga
    11221 cgccgtccgc ttccgctgta aagttagtaa tcaggcgatg ccgcaatact ggcttgacga
    11281 ccgcacgtac gtcatcaata tccggtgtga atcgcccgtc caacgcagca cgggttttgg
    11341 cgcctaggat gagatactgc gatgcgcgcg ggcctgcccc ccagctcagc caatcggata
    11401 taaacttcgg tgcacccggt gttttagggc gggtcttcga taccaaatcc acggcataac
    11461 gaacaacatt gtccgctacg ggcacgcgac ggatcagatt ctgataaaaa agaatgctct
    11521 cggcatccaa gactttttcc agagcgtagt gatacgaact ggtggtattc ttgacaatcg
    11581 ccacttcttc gtccgcgctc ggataatcta cccaaatatt aaacataaaa cgatccagtt
    11641 gggcttccgg caaaggatat gtgccttcct gttcgatagg attttgtgtc gctaacacaa
    11701 aaaacggttc ttccagatgg tatgtattcc ccgaagccgt cactttgtgt tcctgcatcg
    11761 cctccaaaag ggccgactgc gttttggggg gcgtgcgatt tatttcgtcc gctaaaacga
    11821 tattggcaaa gatcgggccg cgaacaaaac gaaattgttt actgccggtc gtcatatttt
    11881 cttcgatcac ttccgtgccc acgatatcgc tcggcatcag atcgggcgta aactgaatac
    11941 ggttgaattt taaatccaaa gcccgtgcaa tcgtattgat cagcaacgtc ttggcgagac
    12001 cgggaatacc cacgagcaga cagtgtccgc gcgcaaacaa agcgatggta agttgctcta
    12061 cgatagcgcg ctgacctaca atggctttgg ctacttcggc ttcgatcgcg cttcgggact
    12121 ggcgcaaccg tgcaatcgca tcggtatcgg taagttgttg cagttcggaa aaatttactt
    12181 gcggcgctgt catgtagttc ctttactaaa ttctgagttt tctgcccatt ctacgatcgc
    12241 atcatcggcg tgcgtccatt cgtccccttc cagccaccaa tgagacgcac aaacagggca
    12301 ccgaaaaaga gcccgatcac gcgtttcctg acggattagt ttcatctgtt tagcgtcggg
    12361 tgcaaacgat aaaaattctt tacgacgctg gaattgttca tatggtttta gacgcaccca
    12421 ttggccggtt tgttcttcag taaatcggac gaaagcagcg caatggacgc attgatacaa
    12481 cgtgtttttt tcctcacgcc acaccggtcg taactgatct ttggaacgcg ccagataaag
    12541 cgcatccgtc caataatatt ggccttgcgt tcgaaacata ctctccttcc aaaactcttg
    12601 tattgtaata atttagccaa cgataatcaa tatgaataac tggccacgtt ttttcaaatt
    12661 gtgacatgaa aatacaaaaa aacttcatga ctttatgtac gcagtgtaca caaaatcatg
    12721 aagcttaact tggtaatgaa gagattttct acaaaccgtc cacctttaca tcacgcggat
    12781 actcaacgac atacttgata cgcaattcga ttttttcttt aggtttgatc cgaagtcgcc
    12841 attgcactaa accgccacct gttttttcaa taacgccatt aggatgcggt gatgaccatt
    12901 tttcttttgg aaattcaggt aaaacggcct ctactttgat acgttcgtcc tgcgactgcg
    12961 gaaactggtc cacaacaagg atcgtatcat cggttcgttt gaaactctcg attgtaattt
    13021 tatattcgaa agtcttttta accgatttat taaaaatacc tttttcggca ttgtaatcac
    13081 gaatctgctg gcgtttgatg cgaatcccgt cgtcaattcc cagcgaaacg tcaaattttt
    13141 ccgtcggaat aaccgtattc aagtacgttg taccgacaaa attacttccg aaaaatacat
    13201 tggcattacc ggcgagaaag ggcgcttccg tcgtattttc gatacttgca cgcagatata
    13261 caagttcggc gagcttggga acagacgaat acgcaaaatc agattttaag gattcggagg
    13321 taatggtgac tttgtgatcg taattatccg acgggatatt ggtcttgcgt ttgattttaa
    13381 aaacaaccga agtgcttttt gtttcgatgg ctgcttcgtc ggtttccatt tcatagatgg
    13441 gcgcttctgc ctcttcaggg acctctccgg ctccgcttga agcactcatg tctgaaattt
    13501 cctccgcaga tttagtcatc ttggccgaac tcattgcacc catacctgcg cctgaacgtc
    13561 ccccgcgacc caatatgccg atgggttgat agacactgag attccaggct gaaagctgcg
    13621 gcatagagcc ggaaacattg ggccgcgctg tagaaattgc cagatttaca ttactccaat
    13681 cctcgccggt tctctgagcg atcatcgcat aatacgtcat atctacggac ttgtcctccg
    13741 gcgatacacg cacatcatag accggatacc actttgcgcc accgattaca taatctatgg
    13801 caaaattcat catacccggt ttactcacat tgaccgtcaa aaaaactttt ttctttacca
    13861 atttgccgta accggataat tgattgatct gattttgcag cgcattttta cgtacggtca
    13921 gatcaccgcg ctttttctcg agcatgcgaa tttcatcatt aagtttgttg taattggcat
    13981 cgtaaaatcc aaatagtttg gaccaatcgt caaaactgag tttgttggat tctttattct
    14041 gcgtgatgct atttttgatc tgatcaagca attctttttc tttaccgagc aatccgagac
    14101 ggtcgttaaa cacacgttcg tcttgcatta acaatgcaag ctgatcctgc agatctttga
    14161 ggcgattttg aggcattgtg tctatataat cgagttcgat tttgatgtcg gatatccgcg
    14221 ccatggccgt accactgcct ccgacacgca acgactgatc ttgcagcgca tggggcaaat
    14281 ccgaaacgac gaattgatac tcgcccggat tgagataaat ttccccgctg cgcgtgattg
    14341 ccgcacggtc ggtatatacc gtaacatcgc tgattgcggt gttcagtttt tgttgagtcc
    14401 atcctgtaga ctgcgtcagc agaaacagag ccaataaatg gaagaaacgg ctacggatca
    14461 tgataataac tcctcgttat tttataaata aacctacgtt gtattcttaa cga
//
LOCUS       DAOQGF010000088        14290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1958_length_14290_cov_7.103196, whole genome shotgun sequence.
ACCESSION   DAOQGF010000088 DAOQGF010000000
VERSION     DAOQGF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_14290_cov_7.103196"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK937_11605"
     CDS             complement(<1..509)
                     /locus_tag="PK937_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017252397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNH33260.1"
                     /translation="METEVYKPKHKIRFVTAASLFDGHDASINVMRRILQSSGAEVIH
                     LGHNRSAEDVVNTAIQEDAQAIAMSSYQGGHVEYFKYMRDLLNQKGAKHIKIFGGGGG
                     VIVPDEIKELEAYGIDRIYSPEDGRIMGLQGMINDMLRRTDFPTIDHVNGEVDALPKK
                     NPGTIAKLIT"
     gene            737..1267
                     /locus_tag="PK937_11610"
     CDS             737..1267
                     /locus_tag="PK937_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33261.1"
                     /translation="MSNQTIVIRDAMSSEFSLVGQLMVEVYAGLKGFPKPTEQPAYYK
                     MLSAVGELTLKPDTALRVAVLNDGTIAGAVVYFGDMQYYGSGGTATQEKNSAGFRLLA
                     VNPEFRGGGIGKLLTLDCIQRAKAQRRVQMIIHTTMAMRTAWAMYEKLGFQRSVDLDF
                     MQGELAVFGFRLPLNA"
     gene            complement(1291..1989)
                     /locus_tag="PK937_11615"
     CDS             complement(1291..1989)
                     /locus_tag="PK937_11615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNH33262.1"
                     /translation="MFPIRDTIPSSRFPLVTTVLIVINSLIFLYEVSLGEQLNRLINA
                     LGIIPAKYFWQGEFGRANFIERFFPFFTSMFLHGGWMHAIGNMWYLWIFGDNVEDRMG
                     RGRFLLFYLLCGLGAGLSHAYLNADSRIPTIGASGAIAGVMGAYIVLFPNSRVLTLVP
                     IFIFIQFFEIPAIFFLGFWVILQFLQGTASLMSDASGGVAWWAHLGGFVFGAILVFVF
                     RQPERRRWYDEEYE"
     gene            2502..3614
                     /gene="dnaN"
                     /locus_tag="PK937_11620"
     CDS             2502..3614
                     /gene="dnaN"
                     /locus_tag="PK937_11620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNH33263.1"
                     /translation="MKFTVAQADLAKGLARVISVVPSKSTLPVLSNVMLKAQDGFLTL
                     TATDLDVSITTKIQVDVKQPGSITVPSKPLNDLVKSLPNIPLEVSADAQFKFFVHCDN
                     EEYRIPGESDDDYPSLPTVDEKGSLTIEAKQLSRMIGKTIFAVSNDQLRPALTGVLFQ
                     IHGSEFRLVATDGHRLGKMVCTKFETTIKEKTNVIVPTKALSEVAKDTKDATKIIFGQ
                     NHICFDMGDTQLFGRILDENYPDYERVIPGDNNKKLTVDAATLVEKVRRASIFANPIT
                     HQIRLAMDKDGARISAEDVDGGGKGSNQIKAEYDGDALQIGYNAQLLLSALNNIDTQD
                     VVIAYKGPTTAGVITPSHQLEHENQMMLVMPVRLND"
     gene            3635..4768
                     /gene="recF"
                     /locus_tag="PK937_11625"
     CDS             3635..4768
                     /gene="recF"
                     /locus_tag="PK937_11625"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNH33264.1"
                     /translation="MLIRRLRLRDFRNYTDAEIQFAPGLNVITGDNGQGKTSLLEAVY
                     YLCLTKSFKTTEDESAIRFNTSYFDIEGQLARDESSLENDEIRLVVHRAEGKSLLVGK
                     KRQDRFSEHIGRFPAVISAPEDVAIVSGAPGNRRRWLDMALSQLYPVYLKDLLDYRQA
                     LKQRNALFAATFLRPDLLAVWDRELATVGARIIRRRLEFVRSFAGWAARVYGELAVVS
                     EQASVSYKSTIANADTINRWCETLPDDQSIAEAMAKTIAENRDREMMRQTSLIGPHKD
                     ELLLLLNDKAIREFGSQGQLKTMALALKLAEFLHMAERRELQPLLLLDDVFSELDWTR
                     RHNLIRYLENAGQVFLTSADTERDFETKRPIRHFKVINGSVSA"
     gene            4765..5061
                     /locus_tag="PK937_11630"
     CDS             4765..5061
                     /locus_tag="PK937_11630"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNH33265.1"
                     /translation="MKPKKTDATPLGEALDKLLKRYKIADRVHEQVVLEQFERLMGTA
                     FTQRAKAVKIEDGLLFLEVVNSVWRQELFYQKRNIIERINSALGEKIVREIVFR"
     gene            5079..5468
                     /locus_tag="PK937_11635"
     CDS             5079..5468
                     /locus_tag="PK937_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNH33266.1"
                     /translation="MHKDVKAYHGQLSDEDRLICETLYAEISRGLPEAENKVWHAHPV
                     WFLDGNPIVGYSKLKSCIRLLFWSGQSFDEPGLQNEGRFKAAEKRFTSADQVIKKDLA
                     RWLKKAKTIQWDYKNIVKRKGVLERLK"
     gene            5521..7455
                     /gene="gyrB"
                     /locus_tag="PK937_11640"
     CDS             5521..7455
                     /gene="gyrB"
                     /locus_tag="PK937_11640"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNH33267.1"
                     /translation="MAEKEQKKTGAKYDAENIQVLEGLEHVRRRPAMYIGDVSTRGLH
                     HLVYEIVDNSIDEAMAGYCTEINVSINEDNSITVVDNGRGIPVDMHPTEKKPAVEVVM
                     TVLGAGGKFDKNTYKVSGGLHGVGASVVNALSEWCEVEVRRNGKMYRQKYKAGRSTTK
                     VDEVGKSKESGTTTTFMADSTIFKKIVYTFDTLAARMRELAFLNRNLKITLEDKREKN
                     RREEYHYEGGISQFVQYLDESRAALMKKPIYIETEKENVPVEIAFQYNDSYNENVFSY
                     VNNINTVEGGTHLSGFRTALTRTLNNYATKNNLVKEKEGISIQGDDTREGLTAIISVK
                     IAEPQFEGQTKTKLGNSEVKGIVEQAVGEGLMEFLEKSPGVAKQIIDKCLQAARAREA
                     ARKARDLTRKKNMLESSGLPGKLADCSISDPQYCEIYLVEGDSAGGSAKQGRDRRFQA
                     ILPLKGKILNVEKARLDKMLSNEEILTIVSAIGTGIGTDDFNLANLRYGKVIIMTDAD
                     VDGAHIRTLLLTFFFRHMRQLVENGNLYIAQPPLYRLKKGKEEKYAFDDEEKAAMLKQ
                     WGAKEEDTNIQRYKGLGEMNPEQLWSTTMDPDKRVMMQVTIEDAATADHVFSTLMGDA
                     VEPRRKFIEDNAKYVRNLDV"
     gene            7496..9943
                     /gene="gyrA"
                     /locus_tag="PK937_11645"
     CDS             7496..9943
                     /gene="gyrA"
                     /locus_tag="PK937_11645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNH33268.1"
                     /translation="MSTIEKLVPRNIEDEMRECYIDYSMSVIVSRAIPDVRDGLKPVH
                     RRVLFGMNELGMSYNKPYKKSARIVGEVLGKYHPHGDTAVYDTIVRMAQPFSLRYMLV
                     DGQGNFGSIDGDSPAAMRYTECRLSRIAEEMLRDLEKNTVNFVPNFDDTLKMPEVLPA
                     VMPNLLVNGSTGIAVGMATNIPPHNLTEVVDGLHALIDDPEITIKDLMKHIKGPDFPT
                     AGIINGVAGIKEAYETGRGKIIIRARANIETNKNNRQSIIITELPYTVNKANLVEKIS
                     DLVREKKVEDISALRDESDRDGIRVVIELKRDANAQVVMNNLFKHTQMQTTFGIIILA
                     LVDGRPMVLNLKQTLQHFIDHRHEVVQRRTQFELEAAERRAHILEGLKIALENIDAII
                     QLIKKSKDPAVAKEGLMKKYKLSEIQATEILKMQLQRLTNLEVEKIENEYRDVIKLIE
                     KLKGILASKAKRMAIIKDELADIRKTFGDERRTEILKKEVEEIDFEDTIAEEEVVVTI
                     SRQGYIKRFPVSGYRRQGRGGQGVSGGQTKDDDFIEHLFVASTHEYIMFFTDKGRCYW
                     LKVYEVPELSKTARGKHISNLIEQQSDEKIRAVINVKEFSDQLSVLMVTKEGVIKRTN
                     LSEYSNPRKGGIIAITIEKNDDLVDAQLTDLKQDVLLGTHDGIAIRFHGEEVREMGRS
                     ARGVTGINLEKGDYVVGMVSLKRENGTILVVSDMGYGKRSEVGDYRTTRRGGKGIISM
                     KATDKTGAMIAIREVVDNEDLMIITQNGMVIRLKMADIRTMGRNTQGVRLVKLKEDDT
                     ISDITSIKEEESEEAQD"
     gene            10028..11461
                     /locus_tag="PK937_11650"
     CDS             10028..11461
                     /locus_tag="PK937_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33269.1"
                     /translation="MFETIVDGIYEQIRQKMHTDQNHWSYFELTGKGSDEVVRKIFTG
                     KYEQIFRAEKARIVAARINTALPYGKAPVDKALDILVDELKREWLIPKTEVEQTLRES
                     LLLRLQYITMPLATVESLMFADTPARTIEEIVNQYRAFEIYKYYPDALERYAKAKAIT
                     HLNKIQLRLLINEINQSLFGKDALENVLKLCGLVMKEVNELRGASTNTIEIGLLMKAF
                     ADRSLRDFEVALNIEKELGNEQINIYGLRQVLNRFVILKEKKAVAAEKTIVNLAPKSE
                     PSPVESRLAVKPKDTTDLVIDKVRKDEESGEIIDLNAILSHQPSKEKTDELRPKPKEP
                     TVETIAFPADMKAKTGSTKIKFAPETVEEVTGEFIKEELVQGFSDDTQTIRLKDIKES
                     DNLVLLQSVITPKDEQIFMKSIFGGDEAYYRDFVSQVNRSKTWKEAITVVDDMLFEKK
                     VDPYCKEAIRLSDVVYMRYYPPEKNNG"
     gene            11474..11620
                     /locus_tag="PK937_11655"
     CDS             11474..11620
                     /locus_tag="PK937_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNH33270.1"
                     /translation="MENLGYLFAAFAAIWVLLFYYVQRMNSKQKQMMKDLEALRQIVE
                     EKKG"
     gene            complement(11630..12373)
                     /locus_tag="PK937_11660"
     CDS             complement(11630..12373)
                     /locus_tag="PK937_11660"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNH33271.1"
                     /translation="MQLRVASITDRGLNPSRTHNEDSFFTGNQLYIVADGLGGELAGE
                     VASQMTIEKFNEWFPDATRESANEAGSELAAIERTVKRVNAYVYQEAQKPEFKNMGST
                     LSILYFFRNHALIGHVGDSKIYRVRNGKTEQLSHDQSMVQQLLDKGIITTEQAKRHPM
                     RNVLLSCIAHKPDIDVFTYDTDIQNGDFYLLCSDGVSEYVGHGAIAEMAGNGFSGEEI
                     CATIKNIVYAGGAMDNMTAIVIFVDQVDA"
     gene            12535..12927
                     /locus_tag="PK937_11665"
     CDS             12535..12927
                     /locus_tag="PK937_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33272.1"
                     /translation="MKALYLFLMSVVLTGIVSAQDKSYNTDQLKSAKRISEDQWKGML
                     DASSQRSNGFVTAAKGTTDYMATQLGYTVLKYNQTIELKAEVIAVLEGVYSAGQSNAG
                     TAGTRNPKTFKLGPGKYEVKEGIIKRVP"
     gene            complement(13112..13519)
                     /locus_tag="PK937_11670"
     CDS             complement(13112..13519)
                     /locus_tag="PK937_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33273.1"
                     /translation="MIVKSTLLLLWALIAACSASRTQVEAQQDAEKYKLIAIEKFSKD
                     IKYVFNSTRTHVLCIKETRPEHPPKIHVMSFLLIEAITGHIILEETTTNAQAEWISES
                     EVRVIFITGMPKENSHRAVGYIYNVISKVKTDL"
     gene            13815..>14290
                     /locus_tag="PK937_11675"
     CDS             13815..>14290
                     /locus_tag="PK937_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNH33274.1"
                     /translation="MSLTHRSHKDKNFDLLRLIGVRGMGHARLRNLIRHFGSPASVLS
                     ASPKALAAVQGMDAPTAERIKQDHEVSIAFAHAQMRSMQENATELLIFSESGFPELLT
                     QTETAPSFLFVRGAIMPEDAHAIAIVGTRQPTAYGRTMAEKLAQELAQRGITVVSG"
BASE COUNT         4080 a         3122 c         3478 g         3610 t
ORIGIN      
        1 gtgatcagtt tggcgatggt gcccggattt tttttaggta aagcgtccac ttcgccgttg
       61 acgtgatcga tcgtcggaaa atccgtacgc cgcagcatat cgttgatcat gccttgcagc
      121 cccatgatgc gaccgtcttc gggggagtat atccggtcaa tgccgtacgc ttcgagttct
      181 ttgatttcgt cgggaacgat cacaccgccg ccgccgccga aaattttgat atgtttggcg
      241 cctttttgat tgagcagatc gcgcatgtat ttgaagtatt cgacgtggcc accctgatac
      301 gaactcatcg cgatggcttg cgcgtcttcc tgtatggccg tattgactac gtcttcggcg
      361 ctgcgattgt gtccgaggtg aatgacttcc gcgcccgacg attgcaggat acgccgcatg
      421 acattgatgc tcgcgtcgtg cccgtcaaaa agactggccg cagtaacaaa tcggatttta
      481 tgtttgggtt tgtaaacttc cgtttccatg ctgtatcctt aaaaaataga ttttgcgtat
      541 ttttctcata cgtacgaaac ttaccaaaat atgaacaaat tctcaatgtt tgtatgaatt
      601 tattgtattt tgaatcccaa cgtaggtaag tttaaatgtc tctacgacaa cacattggtg
      661 ttctatgata atttaaaaca aaaatatcgt tgtgattttt ggaaaggcta aaactatagt
      721 ttttaaggtt aaatgcatga gtaatcaaac aatagtgatt cgtgatgcga tgtcgagtga
      781 gttttcgctt gttggtcaat tgatggtcga agtgtatgcc ggactgaagg gatttccaaa
      841 accaacggag caacctgcgt attataaaat gttatctgct gttggcgaac ttacgctgaa
      901 accggatacg gctttgcgtg tcgccgtttt aaatgatggt actattgctg gtgctgtcgt
      961 gtattttggc gatatgcaat attacggttc cggcggaaca gccacccaag aaaagaattc
     1021 agccgggttt cgacttttgg cggtgaatcc agaatttcgc ggtggtggta tcggtaagct
     1081 tctgacgttg gattgcattc agagagccaa ggcacaacgc cgtgtacaaa tgatcattca
     1141 tacgaccatg gccatgcgta cagcctgggc tatgtatgaa aagctgggat ttcagcgttc
     1201 tgtagaccta gattttatgc aaggtgaatt ggctgttttt ggtttccgtt tgccacttaa
     1261 tgcgtgagca ttgaaaaatc atggtatacc ttattcgtat tcctcatcgt accatcttcg
     1321 ccgttccggt tgacggaaaa caaaaaccag aatcgcgcca aatacaaacc cacccagatg
     1381 cgcccaccag gcgacgccac ccgaggcatc ggacatgagc gaagccgttc cttgtaaaaa
     1441 ttgtaaaata acccagaaac cgagaaaaaa aatggcggga atttcaaaaa actgaataaa
     1501 aatgaaaatc ggcaccagtg taagtacacg cgaattcgga aataatacga tataagcgcc
     1561 catcacgccg gctatggcac cgctggcgcc gatcgtaggt atgcgcgagt cggcattgag
     1621 gtacgcatgc gaaagcccgg caccgagtcc gcacaaaaga taaaacagca aaaaacgccc
     1681 gcgacccatt cgatcttcca cattatcgcc gaaaatccac agataccaca tattaccgat
     1741 ggcgtgcatc caaccgccat gcaaaaacat ggatgtgaaa aacggaaaaa agcgttcgat
     1801 gaaattggcg cgaccaaact caccttgcca aaaatatttt gcaggtatga tgcctagcgc
     1861 gttgatcaat cggttgagtt gctcgcctaa cgatacttca tacagaaaga tcaggctatt
     1921 gatcacgatc aatacagtcg tgaccagcgg aaaccgggaa gaagggatgg tgtcgcgaat
     1981 gggaaacata gtttaatgtg tatgcttgtc cgtatttttt gtgtacgtgt tttgggttat
     2041 cgttccgaaa atatgataca tggtgtttaa tagttatgga tcgtattgtt caaaaacacc
     2101 ttcccttgtt tgaatgcgtg gcgcctgtaa cactaaaacg aaaacgtgaa caaataaaat
     2161 aaacggtttg tattatcgcg cttcgcgcaa aaattatttt acacaaacag gcaaaacaaa
     2221 ttaataacat tcgccgataa aacggaacta ttttttataa aaaacaaggg catgtacgtc
     2281 atacaaacgc gcatggaact ttacggatag ggtgtttttg gcggcgataa tcataatatt
     2341 ttgcttgaat ttttgacaat aaaacactat gattttcagt tttaaaaata cccgtcaacc
     2401 cttgattttg ttgaatttca cggtgaaata aaacccgtcg tttggcacta taattttttg
     2461 aatagaatat atttgttatt tagccacagg agttgcagga tatgaagttt acggttgctc
     2521 aggccgatct ggccaaagga ttggcgcgtg ttatcagcgt cgttccctca aaatccacgt
     2581 tgcccgtgct gagcaacgtc atgctcaaag cgcaagatgg tttccttacg ttaaccgcta
     2641 cggatttgga tgtttcgatc accaccaaaa ttcaagtgga tgtcaaacaa cccggaagca
     2701 tcacggtacc gtccaaaccc ctcaacgatc tggttaaatc cctgcccaat attcctctag
     2761 aagtttccgc cgatgcacag ttcaaatttt tcgtgcattg cgataatgaa gaataccgca
     2821 ttcccggtga atcggacgac gattatccgt cgcttccgac cgttgacgaa aaaggttcgc
     2881 tgaccatcga agccaaacaa ctttcccgca tgatcggcaa aacgattttc gccgtcagta
     2941 acgatcaatt gcgtccggct ttgacggggg tactttttca gattcacggt agcgaatttc
     3001 gccttgtagc taccgacggt catcgcctgg gtaaaatggt atgcaccaaa tttgaaacca
     3061 cgatcaaaga aaaaaccaac gtgatcgtgc cgaccaaagc tttgtctgaa gtagccaaag
     3121 acaccaaaga cgcgaccaaa attattttcg gacaaaatca catttgtttc gacatgggcg
     3181 acacccaact tttcggacgt attttggatg aaaattatcc ggattatgaa cgtgtaatcc
     3241 ccggcgataa taataaaaaa ttgacggtgg atgccgctac gcttgtcgaa aaagttcgcc
     3301 gtgcttcgat ttttgccaat ccgattacgc accagattcg tctcgcgatg gacaaagacg
     3361 gcgcgcgcat ttccgccgaa gacgtggacg gcggcggcaa gggttccaac cagatcaaag
     3421 ccgagtatga tggcgatgcc ttgcagatcg gttataatgc gcagctcctt ttgagcgcac
     3481 tcaataatat cgacacgcag gatgtcgtca tcgcgtacaa aggtcccaca acggccggtg
     3541 tgatcacgcc gtcgcatcag ctcgagcatg aaaatcagat gatgctggtg atgcccgttc
     3601 gcctcaacga ttaacgcgga ggctgacccg ttccgtgctc attcgccgtc tcaggctccg
     3661 ggattttcgc aattataccg acgcggaaat acagtttgct ccgggtctca atgtgattac
     3721 gggcgataac ggacaaggca aaacgtcact gctggaagcc gtttattatc tttgcctcac
     3781 taaaagtttt aaaacgacgg aagacgaatc ggccatccgg tttaatacgt cgtattttga
     3841 tatcgaaggg caattggccc gcgatgaaag ttctttggaa aatgatgaaa tacgccttgt
     3901 cgtacatcgt gcggaaggca aatcgctttt ggtcggaaaa aaacgtcagg atcgtttttc
     3961 ggaacatatc ggacgtttcc cggcggtgat ttccgctccg gaagatgtag cgatcgtgtc
     4021 cggcgctccc ggtaaccgac gccgctggct tgatatggcg ctgtcgcagt tgtatccggt
     4081 gtacctcaaa gatttgttgg actatcggca ggctttgaaa cagcgtaatg ccttatttgc
     4141 cgccacgttt ttaagaccgg atctgcttgc ggtttgggat cgtgaattgg ctacggtcgg
     4201 agcgcgtatt attcggcgcc gattggagtt tgttcggtct tttgccggat gggcggcgcg
     4261 cgtatatggc gagttggccg tggtatccga acaggcatcc gtcagttata aaagtacgat
     4321 cgccaatgcg gatacgataa acaggtggtg cgaaactttg cccgacgatc aaagtatcgc
     4381 ggaggcgatg gctaaaacaa tcgctgaaaa ccgcgatcgc gaaatgatgc gccaaacttc
     4441 gctgatcggg ccgcacaaag acgaactttt gcttttactt aacgacaaag cgatacgtga
     4501 gtttggttcg caaggacaac tcaaaaccat ggcgctggcg ttgaaactgg ccgagtttct
     4561 acatatggcc gaacgccggg agttgcaacc tctgcttttg ctggatgatg ttttttcgga
     4621 actggattgg acaagacgtc ataatcttat ccggtatctt gaaaacgccg gtcaggtttt
     4681 tttaacaagc gccgatacgg agcgtgattt tgaaaccaaa agaccgatcc ggcattttaa
     4741 agtgataaac ggaagtgtaa gtgcgtgaaa cccaaaaaaa cagatgcgac gccgctcggt
     4801 gaagcgctgg ataagttact caaacgttat aagatcgcgg atcgtgtaca cgagcaggtc
     4861 gttctggagc agtttgaacg cctcatggga acggcattta ctcagcgcgc caaagcggtc
     4921 aagatcgaag acggactgct ttttttggag gtggtcaata gcgtgtggcg gcaggaactt
     4981 ttttatcaaa aaagaaatat tatcgaacgg atcaattccg cgttaggtga aaaaatagta
     5041 cgcgaaatag ttttccggta attttgaaat aatgcgttat gcataaagat gttaaagcct
     5101 atcacggcca actctccgac gaagatcggc tgatctgcga aacactgtac gctgagatta
     5161 gccgcggatt gccggaggcc gaaaacaaag tatggcatgc gcatccggtt tggtttcttg
     5221 atgggaatcc gattgtcgga tacagcaagc tgaaaagctg cattcgattg cttttttgga
     5281 gcggacagtc atttgatgaa cccggtctgc aaaatgaagg ccgtttcaaa gcagcggaaa
     5341 aacgttttac aagcgcggat caggttatca aaaaagactt ggcgcgttgg ttaaaaaaag
     5401 ccaaaacgat acaatgggat tacaagaata tcgttaagcg caagggcgtg ttagaaagat
     5461 tgaaataaga accgtcagaa aaataaatca taatcataga ttaaaaggac taaaggattc
     5521 atggcagaaa aagaacagaa aaaaaccggc gcaaaatacg acgctgaaaa tattcaggtt
     5581 ctcgaaggac tagagcatgt tcgtcggcgt cccgcgatgt atatcggcga tgtcagtacc
     5641 cgcggtttgc accatttggt gtacgagatt gttgataact ccattgacga ggcaatggcc
     5701 ggttattgta cggagatcaa tgtttccatc aatgaagata attcgatcac tgtcgtggac
     5761 aatggccgcg gtattcccgt ggacatgcac ccgacggaaa agaaacctgc ggtcgaagtc
     5821 gtcatgaccg tgctcggcgc cggcggtaag tttgacaaaa atacgtacaa agtctccggc
     5881 ggtttgcacg gtgtcggcgc gtccgtcgtc aatgcgcttt ccgaatggtg cgaagtcgaa
     5941 gtgcgccgaa acggaaagat gtatcgtcag aaatataaag ccggccgttc gacgaccaaa
     6001 gttgacgaag tgggcaagtc caaagaaagc ggtacgacaa cgacgtttat ggccgacagc
     6061 acgattttca aaaaaatcgt gtacactttc gacacattgg ccgcacgtat gcgcgaactg
     6121 gcatttctca accgcaatct gaaaatcacg ctggaagata aacgcgaaaa aaatcgtcgc
     6181 gaagaatacc actacgaagg cggtatttcg caattcgtcc aatatcttga cgaatcgcgt
     6241 gcggccttga tgaaaaaacc gatctatatc gaaaccgaaa aagaaaacgt gccggtcgag
     6301 attgcatttc agtacaacga ttcctacaac gaaaacgtat tcagctacgt caataacatc
     6361 aatacggtcg aaggcggtac acacctttcc ggttttcgta cggcgcttac gcgtacgctg
     6421 aataactatg cgacaaaaaa caatttagtc aaagaaaaag aaggcatctc gattcagggc
     6481 gacgataccc gcgaaggcct gacagcgatt atcagcgtta agatcgccga gccgcaattt
     6541 gaagggcaga ccaaaaccaa actgggtaat agcgaagtta agggtatcgt ggaacaagct
     6601 gtcggcgaag gcctgatgga gtttctcgaa aagagtcccg gtgtcgccaa acagattatt
     6661 gataagtgtc ttcaggccgc gcgtgcccgc gaagctgcac gtaaagcgcg tgatcttacg
     6721 cgtaagaaaa acatgctgga gtcatccggc cttcccggaa aattagccga ttgttccatc
     6781 agcgatccgc aatattgtga aatttatctc gtcgagggtg attccgccgg cggctccgcc
     6841 aaacaaggcc gtgatcgtcg ttttcaggcc attctcccgc tgaaaggtaa aatcctcaac
     6901 gtcgaaaaag cgcgcctcga taaaatgctt tccaatgagg aaattctgac catcgtaagc
     6961 gcgatcggca cgggcatcgg taccgatgat tttaatctcg ccaatctgcg ctacggcaaa
     7021 gtcattatca tgaccgatgc cgacgtggac ggcgcgcata ttcgcacgct gctgctcacg
     7081 tttttcttcc gtcacatgcg ccaattggtc gaaaacggta acttatatat cgcccagccg
     7141 ccgctatatc gcttgaaaaa aggtaaagaa gaaaaatacg cgtttgatga tgaagagaaa
     7201 gccgccatgc tcaaacagtg gggcgccaaa gaagaagata ccaatattca gcggtacaaa
     7261 ggtttgggtg aaatgaaccc cgagcaactt tggtccacga cgatggatcc ggacaagcgc
     7321 gttatgatgc aggtgacgat cgaagatgcc gcgaccgccg accacgtgtt ttctacgttg
     7381 atgggtgacg ccgtcgaacc gcgccgtaaa tttatcgaag acaatgcgaa atacgtgcgc
     7441 aatttggatg tataaaaatt ttattatata acgatagcag gagctactaa acgaaatgtc
     7501 cactatagaa aaacttgttc cgcgtaatat cgaagacgag atgcgggaat gttacatcga
     7561 ttattcgatg tccgtcattg tgtcccgtgc gattccggat gtgcgcgacg gattgaaacc
     7621 ggtgcatcgc cgcgtgctgt ttggtatgaa tgagctcggc atgtcttaca ataagccgta
     7681 taaaaaatcg gcgcgtatcg tcggtgaagt gcttggtaaa taccatccgc acggcgacac
     7741 ggccgtgtac gatacgatcg ttcgtatggc ccaacccttt tcgctgcgct atatgctggt
     7801 ggacgggcaa ggtaatttcg gttcgattga cggcgattcg ccggccgcca tgcgttatac
     7861 ggaatgccgc ttgtcccgta tcgctgaaga aatgttgcgc gatcttgaaa aaaacacggt
     7921 taacttcgtg cccaattttg acgatacgct caaaatgccg gaagtgctac cggcggttat
     7981 gccgaatttg ctggtcaacg gttctaccgg tatcgctgtc ggtatggcga ccaatattcc
     8041 tccgcataat ctgacggagg tggtggacgg attacatgct ttgatcgacg atccggaaat
     8101 cacgatcaaa gatttgatga agcatatcaa aggaccggat ttcccgacgg ccggcattat
     8161 caatggcgtg gcgggtatca aagaagccta cgaaacgggc cgcggtaaaa tcatcatccg
     8221 tgcgcgcgcc aatatcgaga ccaataaaaa taatcggcaa agcattatta tcaccgaact
     8281 cccgtacacg gtgaataaag cgaatcttgt cgaaaaaatt tccgatcttg tgcgtgagaa
     8341 aaaagtcgaa gatatcagcg ctctgcgcga cgaatccgac cgcgacggta tccgcgtagt
     8401 gatcgaactg aagcgtgatg ccaatgcgca agtggtgatg aataacctct tcaagcatac
     8461 gcagatgcaa acgacgttcg gcatcatcat tctggcgctt gtggacggac gcccgatggt
     8521 cctcaatctt aagcaaacgc ttcagcattt cattgaccat cgccacgaag tggtgcaacg
     8581 tcgtacgcaa ttcgaactcg aagccgcaga acgtcgggcg cacattttag aaggtttgaa
     8641 aatagcactt gagaatattg atgcgatcat ccagctgatc aaaaagtcca aagatcccgc
     8701 tgtggctaaa gaaggcttga tgaaaaaata caagctttcc gaaattcagg cgacggaaat
     8761 tctcaaaatg cagctccagc gccttacgaa cctggaagtc gaaaaaatcg aaaacgaata
     8821 ccgcgatgtt atcaagctca ttgaaaagct caaaggtatt ctcgccagca aagccaaacg
     8881 tatggcgatc atcaaagatg aattggcgga tatccgtaaa acctttggcg atgaacgccg
     8941 tacggagata ctcaaaaaag aagttgaaga aattgacttt gaagatacga tcgctgaaga
     9001 agaagtggtt gtcaccattt cgcgccaggg gtatatcaag cgtttccctg tgagcgggta
     9061 tcgccgccag ggccgcggag gtcaaggcgt gtccggcggt cagaccaaag atgatgattt
     9121 tatcgagcat ctttttgtcg cttccaccca cgaatacatc atgttcttta ctgacaaggg
     9181 ccgttgttat tggctgaagg tttatgaagt gcctgaactt agtaaaacag cgcgcggtaa
     9241 acacatcagt aacctgatcg aacagcaatc ggatgagaaa atccgtgctg tgatcaatgt
     9301 caaagagttc agcgatcaat tgtccgtgtt gatggtaacc aaagaaggcg tcatcaaacg
     9361 taccaatctt tccgaataca gcaatccccg caaaggcggt atcatcgcta ttaccatcga
     9421 gaaaaacgat gatcttgtag acgctcagtt gacggatctt aagcaagatg tattgctcgg
     9481 aacgcatgac ggcatcgcta ttcgtttcca cggcgaagaa gttcgtgaaa tgggtcgctc
     9541 ggcgcgcggt gtcaccggta tcaatttgga aaaaggtgat tatgtggtcg gcatggtttc
     9601 actcaaacgt gaaaatggta cgatccttgt cgtttccgat atgggttatg gcaaacgcag
     9661 cgaagtcggc gattatcgta cgacacgccg cggcggaaaa ggtatcatct caatgaaagc
     9721 caccgacaaa accggggcca tgatcgctat ccgtgaagtg gtggacaatg aagatctgat
     9781 gatcatcaca caaaacggta tggtcattcg tctcaaaatg gcggacattc gtaccatggg
     9841 acgtaatacc caaggcgttc gtctggtcaa actgaaagaa gacgacacga tttcggatat
     9901 tacaagtatc aaagaagaag aatcggagga ggcgcaggac tgatcaggca ctgcgcaagc
     9961 tgtcagtagt tctgacggca catcataata aagggctccc gcgcgcggtg cgcgggtgaa
    10021 acggggtatg tttgaaacga tagttgacgg gatttatgaa caaatccgtc agaagatgca
    10081 tacggaccag aatcactggt cgtattttga actgaccgga aaaggttcgg atgaagtggt
    10141 tcggaaaatc tttaccggta agtacgaaca gatttttcga gcagaaaaag cccgcatcgt
    10201 ggcggcgcgc atcaatacag cccttcctta cggcaaagcg ccggtagata aggcgttgga
    10261 tatattggtg gatgaactga aacgcgaatg gctgataccg aaaacggaag tggaacaaac
    10321 acttcgcgag tcgttattgt tacgtttgca atacatcacg atgccgctgg caacggttga
    10381 atcgttgatg tttgccgata cgccggcgcg tacgatcgaa gaaatcgtca atcagtaccg
    10441 tgcatttgaa atctacaaat actatcccga tgcactggag cgttatgcta aagccaaagc
    10501 catcacgcat ctcaataaaa tccaacttcg tctgctgatc aacgaaatca atcaaagcct
    10561 ttttggtaaa gatgctctgg agaacgtgct caaactgtgc ggtcttgtga tgaaagaagt
    10621 gaatgaactg cgcggcgcgt cgacaaatac gattgaaatt ggtttgttga tgaaggcttt
    10681 tgctgatcgt tcgttgcgag atttcgaagt agcgctcaat atcgaaaagg aattgggcaa
    10741 tgagcagatc aatatttacg gcttacggca agttctcaat cgttttgtta tcctgaaaga
    10801 gaagaaagcc gtggcggccg aaaaaacgat cgtgaatcta gcgcctaaat cggagccctc
    10861 accggtcgaa tccagacttg cggttaagcc caaagatact acggatctcg tcatcgataa
    10921 agtgcgtaag gacgaagaaa gcggcgagat tatcgacctc aatgcgatat tgagccatca
    10981 accttcaaag gaaaaaaccg acgaactgcg ccccaaaccg aaagagccga ctgttgagac
    11041 gatagctttt cctgcagata tgaaggctaa aaccggttcg actaaaataa aatttgctcc
    11101 tgaaacagtc gaagaggtaa cgggtgaatt tatcaaagaa gagctggtgc aaggtttttc
    11161 ggacgatacg caaaccatcc gtcttaagga tatcaaagaa tcggataatt tggtgctttt
    11221 gcaatcggtg atcacgccga aagatgaaca gatttttatg aagtctattt ttggcggcga
    11281 tgaagcgtac taccgtgatt ttgtcagtca agtcaatcgc agtaaaacgt ggaaagaggc
    11341 gatcacggtg gtggatgata tgctgttcga aaagaaagtt gatccgtatt gtaaagaagc
    11401 gattcgattg agcgatgttg tatatatgcg ttactatccg cctgaaaaaa ataacggtta
    11461 aggaataatt tcgatggaga atttaggtta cttgtttgcc gcatttgccg cgatatgggt
    11521 attgctgttc tactatgtgc aacgtatgaa tagcaaacaa aaacagatga tgaaagactt
    11581 ggaagcgttg cggcagatcg tggaagaaaa aaaaggttag cggagttttt tacgcgtcaa
    11641 cctgatcgac aaatatgacg attgccgtca tattgtccat ggcgccgccg gcatacacga
    11701 tattctttat agtggcgcaa atttcttctc ctgaaaatcc gttgcccgcc atctcggcaa
    11761 tagccccgtg accgacatac tcggacacgc catccgagca taacaaataa aaatccccgt
    11821 tctgtatatc ggtgtcatac gtaaagacgt caatatccgg cttgtgcgcg atgcagctca
    11881 gcaaaacatt gcgcatcgga tgacgtttag cttgttcggt ggtaataatg cctttatcca
    11941 aaagctgctg taccatgctt tgatcatggc tgagttgttc ggttttaccg ttgcgaacac
    12001 gatatatctt gctgtcaccg acatgaccga tcagcgcatg attcctgaaa aaatataaga
    12061 tcgataaagt ggatcccata tttttaaatt cgggcttttg tgcttcctga tacacataag
    12121 cgttgacccg tttgaccgtg cgttcaatag cggcgagttc actgccagct tcattcgccg
    12181 attcccgtgt ggcatcgggg aaccattcgt tgaatttttc aatcgtcatt tgactggcca
    12241 cttcgccggc cagttccccg ccgagtccat cggcgactat ataaagctgg tttcctgtaa
    12301 aaaaactgtc ttcattgtgt gtgcgactgg gattgagccc ccgatcggtg atactggcaa
    12361 cacgtaactg catagatgat cgttagattc cgtttttagt gagagcaatc tgaaataata
    12421 gaaaaaagag aatggataat caagaattca ctttgaattc aataatttgt tttgactctt
    12481 tcgttaacgg aagttattat ttgacaatca aactaaggta aaagaggtga cttcatgaaa
    12541 gcgttgtatt tatttttgat gtcagtagtt ttgacgggta ttgtttccgc acaggataaa
    12601 tcctacaata cagatcaatt gaagtcggct aaacgcattt cggaagatca gtggaaaggt
    12661 atgttggatg cgtcatcgca gcggagcaat ggatttgtaa cggcggctaa aggtacgacg
    12721 gactatatgg caacgcaatt aggatacacg gttttaaaat acaatcaaac gattgaactt
    12781 aaagcagaag tgatcgctgt tttagaaggc gtttattctg ccggacagtc caatgccggt
    12841 acagccggta cacgtaatcc taagaccttt aagcttggcc cgggaaaata cgaagtcaaa
    12901 gaaggtatta tcaaaagagt tccgtagttt tgtaatgcga taagtttttg attcgtcttt
    12961 taatttcaac ttccacataa aaaaacctcc cgaaaaaatc gggaggtttt tcgtttatag
    13021 catccgatac tgattaagta gaattaataa tgaattttag aatattaaaa ttcttttacc
    13081 atcattagat agtcaattct aaaaggatat cttacaagtc agtttttact ttagaaatta
    13141 cattatatat gtaaccgacg gcgcgatgac tattctcttt gggcattccc gttataaata
    13201 ttactcgtac ttcggattcg gaaatccact cagcttgagc attagttgtg gtttcttcca
    13261 gtatgatatg acctgtaata gcttcgatta ataaaaaact cattacgtgg atttttggtg
    13321 ggtgctcagg cctggtttct ttgatacata aaacatgcgt gcgcgtacta ttgaatacgt
    13381 atttaatatc ctttgaaaat ttttcgatag caatcaattt atatttttcc gcatcttgct
    13441 gtgcctcaac ctgtgtacgc gatgcactgc atgccgcaat cagagcccaa agcaacaaaa
    13501 gcgtagattt tacgatcata aaatcctcat aataatttaa caaaaagatg cgaacgattt
    13561 cagccgcccc atatacggca tgtactttat aagaacttgt acttgaaagg taaaataatt
    13621 tatctaaaag gattataaag tcaagcggaa atggttaagt agctgttttg aatagttata
    13681 aagtatggta cttttttact tcgcttggga ttcattttta ccaaaaacaa atctgcgcgt
    13741 cttgcattta tgcggagtgc atattacatt acgcggcttt tcgattgggg aacttcaaac
    13801 gaattgattt atctatgagc ttgacgcatc gttcgcataa ggacaaaaac tttgatttat
    13861 tacgcctgat cggtgtgcgc ggtatggggc atgcccgatt gcgcaatctt attcgtcact
    13921 ttgggtcgcc cgcatccgtt ctcagcgctt cgcccaaagc attagctgcc gtgcaaggca
    13981 tggatgcgcc gaccgccgag cgcatcaagc aggaccatga agtaagtatt gcttttgctc
    14041 atgcgcagat gcgatccatg caggaaaacg cgacggaatt attgattttt tccgaatcag
    14101 gatttccgga attactcaca caaactgaaa ccgcaccctc gtttttgttt gtgcgcgggg
    14161 cgataatgcc ggaggatgcc catgcgatag cgattgtcgg aacacgtcag ccgacggcgt
    14221 acgggcgtac gatggcagaa aaacttgcgc aagagttagc ccaacgcggt attaccgtcg
    14281 tcagcgggtt
//
LOCUS       DAOQGF010000089        14171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_1979_length_14171_cov_6.658118, whole genome shotgun sequence.
ACCESSION   DAOQGF010000089 DAOQGF010000000
VERSION     DAOQGF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_14171_cov_6.658118"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1161)
                     /locus_tag="PK937_11680"
     CDS             complement(172..1161)
                     /locus_tag="PK937_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33275.1"
                     /translation="MKKIGILFGMENTFPQAFVDRVNSKKEKGITAELVSIDKVIQGE
                     PCGYDVIIDRISQDIPFYRGYLKNAALSGTAVINNPFWWSADEKFFNNALSIKVGVPV
                     PKTVLLPSNQHPPDTTALSMRNLAYPLDWEGIFKYVGFPAYFKPFAGGGWKNVYKLNN
                     PEEFFKAYNETGTLVMMLQEEIIFTEYFRCYCLGGKDVHVMQYDPRQPHHARYVKGPK
                     TDAVKKLEATVVDYVLRLNRALGYDFNTVELAVRDGIPYAIDFCNPAPDADLKSVGED
                     NFEWIVEHAAQMAIERAKNHKPGQNNLTWGTFVKSFAADKPLTPISQPKSKKK"
     gene            complement(1260..2444)
                     /locus_tag="PK937_11685"
     CDS             complement(1260..2444)
                     /locus_tag="PK937_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNH33276.1"
                     /translation="MNEHTQTHTYIAKTFFGLEPVLSDELKNIGATQIKMLNRAVSFS
                     ASEEIMYKANLWLRTALRIIKPIHEFKAINEEQLYRHTQKMDWSQFLEPKDTFVIDST
                     AHSKFLTHSHYAALKVKDAIVDQLRDQFGERPSIDAENPTLRINLHIAHDDVTIGLDA
                     SGNSLHKRGYRTGTNEAPLNEILAAGMILLSGWDRRAPLLDPMCGSGTIPIEAAMIAL
                     NVPPGHLRTSFGFFTWKDFDPALWKKVRTDAETQRVRNSDVRIFGSDIDRGILDKTRQ
                     NIVNAKMDAHISIAPKSFSDVTPPATPGMIITNPPYGERLKPADLQTLYKMIGDVLKK
                     NFTGWDAWVISGNKDALKQLGLHPSKKLTLYNGALECRFQKFAMYRGSIKQKHQIPND
                     EK"
     gene            complement(2458..2811)
                     /locus_tag="PK937_11690"
     CDS             complement(2458..2811)
                     /locus_tag="PK937_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HNH33277.1"
                     /translation="MQLDGTWQMVSAELAGGAFEDEDVKDMRLIIKGNAYESHVGKDC
                     DKGVLRIFHYDRPMGLDVIGTDGPNAGRTISAIFEVSKESLTICYQLEGNARPLHFET
                     KPGTMLFLVRYKRTA"
     gene            complement(2929..4185)
                     /locus_tag="PK937_11695"
     CDS             complement(2929..4185)
                     /locus_tag="PK937_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33278.1"
                     /translation="MYLHRIFFVAAFLLFAVFGCYRDPGDGSPQLPPSGPRWIEANLW
                     RMALQPDGRWGYDVTDDDHDGNSSGAEFPKNSGNYGLYAGGLMVGAYKDGAPVVLDMN
                     WTTEFTGGRITNTTPTSVDQLEYSSEDNARTYFIGSTSHHDSPDLQSWPSDYGGPWDS
                     LLSKPILISDLDTWASGHDLRADSLSEEAGQSSLGIEVRRMTYSKTGASWENATLIRF
                     QVVNKSDQNYANAYLSWWVDHNLGSTVTNDAVGTDTARNLVYTYNLSPDVTSTGKQYA
                     VGHTLLYFSSSGINSKWNSTYAYYNGNDPVGQTEKYRIQQGGINHKTGSSLDSTFAGS
                     VGGKYVYPGDPVTGTGTLCTQGRNGRLIGNVGPFTLESGKVYDIVVAVIGGEGTDRLN
                     AISDLRTKSDFIRNAFTTISPNMKLK"
     gene            complement(4276..5442)
                     /locus_tag="PK937_11700"
     CDS             complement(4276..5442)
                     /locus_tag="PK937_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33279.1"
                     /translation="MVPIKYIAGGFKRRALTTFITIAGIALVVFVYTAVLMMSYGVEK
                     TLASGGSERNAVIARKSSQGEISSILDGETINVIMTLPQIAKGADGKPLASAEPVVVI
                     NLDRVGGGISNITVRGVEPTAITLRPNIRITEGRMFNTGSREVIVGTSVTERFIGARM
                     GDKIKFANDYWTIVGVMDAKGSAFESEVWGDARQLQAAFNRGNSASTMNVRMADGVTI
                     EEFKQAFENDKRLSQFEPKTEVKFYKEQSEALATFIAVLGTAITIIFSFGAVIGAIIT
                     MYTAVANRITEIGTLRALGFRRRSVLGSFLLETIFISLSGGVIGVIIASFLQFFTIST
                     MNFNSFSELAFSFSLSPGIIINALIFSTIMGLIGGFFPSIRAARLKIVDALRNA"
     gene            complement(5480..6637)
                     /locus_tag="PK937_11705"
     CDS             complement(5480..6637)
                     /locus_tag="PK937_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33280.1"
                     /translation="MLFKMMLRNALRHKLRTILTILGIAIAVGAFGLLRTVITSWYAA
                     VEAAAVDRLITRQAVSFIFPLPYAYRDQILKVPGVEKVGYFNWFQGVYKDKSEFFPRM
                     ACDPETIFDVYPEFLVTPEEKAAFQKERNACVVGADIAKKHNLKIGDVMSIEGDIYPG
                     RWEFVVRAIYQPKTPAIDATQMFFHWTYLDERMKQDMPGREGNVGWYAVKIKDPSESA
                     AVSEAIDAIFKNSNAETKTETERAFNQGFIGAYGAIINGINIMAFMIIGIILLVLANT
                     MIMSARERNREYAVMKTLGFTGKHIFGVVTGESIIIGILGGIAGYAVTLFFVGAFSVA
                     VPKNFFPVFILADITVIQEFSFAVIVGILAGIIPVVQAIKTDIVDGLRYVG"
     gene            complement(6677..7348)
                     /locus_tag="PK937_11710"
     CDS             complement(6677..7348)
                     /locus_tag="PK937_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH33281.1"
                     /translation="MPGLVEIQNVSKAYQRGSMSIPVLQNINLSIADGDFMALMGPSG
                     SGKSTLLNLIAGIDKPTQGSIIVGGTDVASLSESALAKWRSYHVGFIFQFYNLMPVLT
                     AYENVELPLHLTNLSKKERDERIQKVLTIVGLGDRMKHYPNQLSGGQQQRVAIARAIV
                     TDPTLIVADEPTGDLDRTSAEEIMTLMTRLNQELGKTIVMVTHDPHAAEKAKTQRHLD
                     KGTLS"
     gene            complement(7383..8633)
                     /locus_tag="PK937_11715"
     CDS             complement(7383..8633)
                     /locus_tag="PK937_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH33282.1"
                     /translation="MASEAEKVDLSSLRIQREPMKFDPEDKRSSGNLVKWALMVIGVV
                     AIGFIGYMSRNMFKSTPVVQLTTVAFVAQGSGGGVLTASGYIVPQRKASVASKGTGRL
                     VFLGVEEGDRVQSGQVIARLENNDIQAGLEQARANLLVGQAQVEQAEADLERVKRNYE
                     RSETLLKSNVITDADFEIVRAEYRSAQARVTSAKASVAALQAGIHATEVELENTIIRA
                     PFDATVLTKNADVGEIVAPFAAGANSRGNVVTLADMSSLMLEADVSESNIERVKIKQP
                     CEITLDALPEKRYRGEVWKVVPTADRSKATILTKIRFIDIDNRVLPEMSAKVNFLTEA
                     ISDSAMQAKPKLMVNTSAVVERNGRKVALSVVSGKIRAIPVMTGERNGNKTEIVNGLT
                     AGDKVIDNPAADLNDGDAVTTEQK"
     gene            complement(8737..9471)
                     /locus_tag="PK937_11720"
     CDS             complement(8737..9471)
                     /locus_tag="PK937_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH33283.1"
                     /translation="MSTPRLRVIIVDDEALARQLIREYLASHTDISVIEECANGFEAV
                     KAITEINPDLVFLDIQMPKLSGFEVLELTQRKHGVVFTTAYDQYALKAFEVHALDYLL
                     KPFSRERFDAALAHALQHIRDAITWEAPSSLTSDKKTERILIREGVQVHVIPVTQIDY
                     IEAQDDYIAIYATGKCYLKTQTLSDIEKQLDSSRFVRIHRSYIVHVERIARIELYAKD
                     SRTAILRDGKQIPISRSGYDHIKQLL"
     gene            complement(9468..10529)
                     /locus_tag="PK937_11725"
     CDS             complement(9468..10529)
                     /locus_tag="PK937_11725"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH33284.1"
                     /translation="MHPVLGNIRSLAWYILVWLFAGALIAMLIYFTGRGDGTQAFAFA
                     IPLTLIYGFINLSAYYVSRSSPLVSDRRLRVVLTALFNAMLTAFFWLALAMGWNVILH
                     EWQTAIVMDTGTTMIMIVVGTVLYLLSIAIYYVMMMARQAEQAKQNELQSAVLSKDAE
                     LRMLRAQIDPHFLFNSLNSISALTHSDPDGARCMTVELAAFFRQTLALAQKPLITLRE
                     EWNLCEHFLSVEKVRFGNRLTVEMMLPEQLNDILLPPMLIQPLAENAVKHGIAQCVEG
                     GTIRIHALTDGPYAHIIIENPMEEGSSYSEGTHIGLGNIRKRIQNQYGRDARLTTEKQ
                     NRLFVAKLSIPVSPGEASA"
     assembly_gap    10670..10769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10770..11008)
                     /locus_tag="PK937_11730"
     CDS             complement(<10770..11008)
                     /locus_tag="PK937_11730"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNH33285.1"
                     /translation="MKTDSKIPGQVILGVIAIVLGVLFLLDNLRIIEDFRLSRLWPVI
                     FIIFGVLKIRQSRTVPSYFVGSVLMCVGTIMILHRL"
     gene            complement(10992..11375)
                     /locus_tag="PK937_11735"
     CDS             complement(10992..11375)
                     /locus_tag="PK937_11735"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNH33286.1"
                     /translation="MFTHHSHDIKRHLIWGVALIAIGFVVLLDRLHVYDIGPLWKFWP
                     VFLIVGGISDLLSYNKARRIADGVFWILTGGWLLAVQFYAWGLTYRNSWPFLLIAWGV
                     SKITRSLLNKYYFTPQTTTHYENGL"
     gene            complement(11581..12945)
                     /locus_tag="PK937_11740"
     CDS             complement(11581..12945)
                     /locus_tag="PK937_11740"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNH33287.1"
                     /translation="MSTQKPLSGTELMDKIVSLCKRRGFIFQSSEIYGGLNGCWDYGP
                     LGVELLKNYKDAWWKAMTFRDDIEGLDASILMHPRVWEASGHVENFTDPMVDCKECKA
                     RFREDQIEDSMCGNKAYQGRKAHKCMAEGKFTEARQFNLMFKTYIGPLEDSSAVVYLR
                     PETAQGIYVNFHNVKDSSRQKVPFGIAQIGKAFRNEINTKNFLFRTREFEQMEMQFFV
                     KPGTDMEWFNTWKEIRKQWYLSLGFTEDKLKFHAHEKLAHYAAAAFDIEFLFPFGWGE
                     IEGIHNRTDFDLKRHQEYCGKNLDYFDDTTKEKYLPYVIETSAGASRGVMASLVNAYA
                     EEEAPTGDGKTETRVVMKFHPTLAPVKAAILPLVKKDGMPEVSMTLYNEIKKHYKIFY
                     DESATVGKRYRRQDEIGTPYCFTIDSETLQNQTVTVRHRDSMRQERIAMDQVLRFLQN
                     NLNA"
     gene            13145..>14171
                     /locus_tag="PK937_11745"
     CDS             13145..>14171
                     /locus_tag="PK937_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33288.1"
                     /translation="MRMTFFENFRSAVSFTMHNVKARFFHTFLSVLGIVIGVASLVMV
                     LALIEGMEASVRKQILQTTSMNMVIVSSEPFKKSDGVRMRKTDLAILSPVVFDSLKSS
                     LRISHTAQLVSTYGAEVKVDSSKWVGAQINFSSAMVLRTDSLLAGRVLSDEDIHDKKK
                     FAVINRFLAFQSAKDSSLIPALIGKSIHIGAQTYTIMGITAARETTAEAVLPISFMDS
                     AKLTEYPPIGLFETPQIENVIPLQEHIQNYLHTHPVSTGDTLIVASNKSRVDQMAKGF
                     LVFRIVMGFIVGISVLVGGIGVMNVLLISVTQRTMEIGVRKAAGAKRRDILVQFLSES
                     VVVSFLGS"
BASE COUNT         3645 a         3227 c         3361 g         3838 t
ORIGIN      
        1 acgatgtcca gtatttaccg ctcaagccgg tcggatcacc cgccataaca ccaagaccaa
       61 atcttccaga ttgttgtgca gacagagtgg tcacgcaaaa aaagaagatg aagaataaaa
      121 tactgccgtt gcctttcatg gctgaacctc ctgttgatga tgaactaaag atcatttttt
      181 ctttgattta ggttgcgata tgggagtcaa tggtttatcg gccgcaaaag atttcacaaa
      241 agttccccag gtgagattgt tttgacccgg tttgtgattt tttgcccgct caatagccat
      301 ctgtgccgca tgttcgacaa tccattcgaa attatcttcg cctaccgatt tgagatcagc
      361 atccggcgcc ggattgcaaa aatcaatcgc ataggggatt ccgtcgcgca cagccaattc
      421 cacggtatta aaatcatatc ccaaagcccg attgagtcgc aatacataat ctacgacggt
      481 agcttcgagt tttttgacgg catcagtttt cgggcctttg acatatcgtg catgatgcgg
      541 ttgccgcgga tcgtattgca tgacatgcac atcctttccg cccaaacagt aacaacggaa
      601 atattccgta aaaataattt cctcctgaag catcatgaca agtgtaccgg tctcattgta
      661 agctttgaaa aattcctcag ggttattgag tttgtacaca tttttccagc cgccgccggc
      721 aaaaggctta aaataggccg ggaatccgac gtatttgaaa ataccttccc aatccagagg
      781 ataagccaaa ttgcgcatgg acagtgccgt cgtatcgggt ggatgctgat tggaaggtaa
      841 aagtacggtt ttagggaccg gtacgcctac tttgatcgaa agcgcattat tgaaaaactt
      901 ctcatctgcg ctccaccaaa aagggttatt gatcacggcc gtcccgctga gcgcggcgtt
      961 tttcaggtat ccgcgataaa aaggaatatc ttgtgaaata cgatctatga ttacgtcata
     1021 tccgcacggt tcgccctgaa tgactttatc aatcgaaaca agttcggccg tgatgccttt
     1081 ttcctttttg gaattaacgc gatccacgaa ggcttgtgga aacgtgtttt ccataccgaa
     1141 aagaatgccg atctttttca tgataaggct cccatgattg aaaaaatttt taataagttg
     1201 aaaagcttca tgatcgaata gtggggaaat ttatatcaaa cccggtgtgt taaacaaggt
     1261 tatttttcgt cgtttggtat ctgatgtttt tgtttaatgc tgccgcggta catagcaaat
     1321 ttctgaaagc ggcattctaa agcgccattg tataaggtaa gttttttgga aggatgcaat
     1381 ccgagttgtt taagcgcatc cttattgccg ctgatgaccc aggcatccca accggtaaaa
     1441 ttctttttca gtacgtcgcc gatcatttta taaagcgttt gcaaatcggc aggcttaagg
     1501 cgttccccgt aaggaggatt ggtgatgatc atacccggtg tggcgggcgg tgttacatcc
     1561 gaaaaggatt tgggcgctat ggaaatgtgc gcatccattt tcgcattgac gatattctgt
     1621 cttgttttat ccaaaatacc gcgatcaata tctgaaccga aaatacgtac atcggaattt
     1681 ctgactcgct gcgtttcggc atcggttcga acttttttcc ataatgccgg atcaaaatct
     1741 ttccatgtga aaaaaccaaa cgaagtacgc agatgtcccg gcggcacatt gagcgcaatc
     1801 atagcggctt cgattgggat cgtacctgat ccgcacatcg ggtcaagcaa tggcgctcgg
     1861 cgatcccatc cagataaaag tatcattcct gcggctagaa tttcattgag aggagcttca
     1921 ttagttccgg tgcggtaacc gcgtttgtga agcgaattgc ccgaagcatc taacccgata
     1981 gtaacatcat cgtgagcgat gtggagattg atgcgcagtg tggggttctc cgcgtcaata
     2041 gacgggcgtt caccgaattg atcgcgcaac tgatctacga tggcgtcttt gactttcaaa
     2101 gcggcataat gcgaatgcgt caaaaatttg gaatgggccg tactatcaat gacgaaggtg
     2161 tctttgggtt cgaggaactg cgaccaatcc attttttgcg tatggcggta aagctgttcc
     2221 tcgttgatcg ctttaaactc gtgaatcggt ttgatgatac gaagcgcggt gcgcagccat
     2281 agattggctt tgtacattat ttcttcgctg gcgctaaagg atacggcgcg gtttaacatt
     2341 ttgatttgcg tggcgccgat gttttttaat tcgtccgata aaacaggttc aaggccgaaa
     2401 aaagttttag cgatgtaggt gtgtgtctgg gtgtgttcgt tcattagggg acgcgtttca
     2461 ggcggttcgt ttgtagcgca ctaaaaataa catggtgccg ggtttggttt caaaatgcaa
     2521 agggcgcgcg ttgccctcaa gttggtaaca tatcgtgaga gattcttttg atacttcaaa
     2581 tatagcggaa atggtgcgac cggcattggg cccgtcggtg ccgatgacat ccagtcccat
     2641 cggtcggtca taatgaaaaa tacgcagcac gcctttatcg caatcttttc cgacatgcga
     2701 ttcataagca ttacctttga tgataagtcg catatccttg acatcttcgt cttcaaaggc
     2761 gccgccggct aattccgcag aaaccatctg ccatgtacca tccagttgca taaaaaatta
     2821 tccctgatta tcgctttgat ggaacaaaca tcactatttg ttcggcgata atcaaggata
     2881 ttagttgcta taaaggtttt tgacgaaaat tttaagacta aatcaacatc acttcagttt
     2941 catattggga gatatagtcg taaatgcatt tcggataaaa tcggatttgg ttcttaaatc
     3001 tgaaatggca ttgagacgat cggtaccttc gccgccgatt acggcaacta cgatatcata
     3061 aaccttgcct gactcaagtg taaaaggtcc gacgttacct ataaggcgtc cgttgcgccc
     3121 ttgtgtgcac aaagtgccgg tgcccgtaac aggatcgccg gggtagacat attttcctcc
     3181 gacacttccg gcaaaagtac tgtccaaaga agaaccggtt ttatggttga tcccgccttg
     3241 ttgtattcgg tatttttcgg tttgtccgac ggggtcattt ccgttatagt aggcgtatgt
     3301 ggaattccat tttgaattta ttccgctcga actaaagtac aaaagcgtgt gtccgacggc
     3361 atactgttta ccggtggaag tgacatcggg ggagagattg tatgtataaa ctaaattgcg
     3421 cgctgtatct gtaccgacgg catcattggt taccgttgaa cccagattat gatcaaccca
     3481 ccaggaaagg taagcattgg cgtaattttg atcggattta ttgactactt gaaaacgaat
     3541 cagtgttgca ttttcccatg aagcgcctgt tttggaataa gtcatgcgcc gcacttcaat
     3601 ccctaaggaa gattgtcctg cctcttcgct tagggagtcg gcgcgaagat catgtcccga
     3661 agcccacgtg tctaaatcag atattagaat cggttttgaa agtaaactgt cccatggtcc
     3721 gccataatct gaaggccaac tttgtaaatc tggagaatca tggtgcgagg tggaaccaat
     3781 gaaataagta cgagcattgt cttcggatga atattccagt tgatctaccg acgttggcgt
     3841 agtattggta attctgccac cggtaaattc ggttgtccaa ttcatatcca aaaccaccgg
     3901 tgctccgtct ttgtatgcgc cgaccatcaa tccgcccgca tagaggccgt agtttcctga
     3961 gttttttgga aattcggcgc cactactatt accgtcgtga tcatcatccg tgacatcata
     4021 accccaccga ccgtccggtt gaagcgccat gcgccaaaga ttagcttcga tccatcgcgg
     4081 ccccgatgga ggaagttgcg gtgacccgtc cccaggatca cgatagcagc caaaaactgc
     4141 aaaaagtaaa aaagccgcca caaaaaagat acgatgaaga tacataacaa actcctttct
     4201 agttgttagt atactgttgt tgtatcttca tagttcaaaa aacatggtaa ctcttacaaa
     4261 atattttcaa aaaaattatg cgttacgcaa tgcatccacg attttgaggc gtgccgcgcg
     4321 gatggacggg aagaaaccac cgatcaaacc catgatcgtc gaaaaaatca aggcattgat
     4381 gatgatgccc ggcgataacg aaaacgaaaa agctaactca gaaaatgaat taaaattcat
     4441 cgttgagatc gtgaaaaact gcaaaaaaga ggcgatgatc acaccgatca caccgccgga
     4501 caaagagata aaaatcgtct caagcaaaaa gcttccaaga acactgcgac ggcgaaatcc
     4561 gagcgcgcgt aatgtaccga tttctgtgat tcgattagcc acggccgtgt acatcgtaat
     4621 aatcgctccg attacggcgc cgaaactgaa aatgattgtg atggcagttc ctagcacggc
     4681 gataaatgtg gccaacgctt cggattgttc tttgtaaaat tttacttcgg tcttaggttc
     4741 aaactgcgat aggcgtttat cgttttcaaa agcctgttta aattcttcga ttgttactcc
     4801 gtctgccata cgaacattca tcgtagaagc cgaattaccg cgattgaatg cggcttgcaa
     4861 ttgtcgggca tcgccccaaa cctccgattc gaaggcgctg ccttttgcat ccattacacc
     4921 gacaatggtc cagtagtcat tggcaaactt gatcttatca cccatacgtg caccgataaa
     4981 acgctccgtt acggaggtac ccacgatcac ttcacgcgaa cctgtattaa acatacggcc
     5041 ttccgtgatg cggatattgg gtcgcaaagt tatggctgtc ggttcgactc cgcgaacggt
     5101 tatattgctg ataccgccgc caacgcgatc aagattgatc acgacgaccg gttcggccga
     5161 agccaacggt ttaccgtcgg cacctttggc gatttggggg agcgtcatga tgacattgat
     5221 cgtttcgcca tcgagaatac ttgagatttc gccttgcgag gatttgcgtg cgatgacggc
     5281 gtttcgttcg gaaccaccgg aagcgagtgt tttttccaca ccatacgaca tcataagaac
     5341 agccgtataa acaaatacga cgagtgcgat accggcaatc gtgatgaagg ttgtcaaagc
     5401 tctgcgttta aaaccgccgg cgatatattt gataggtacc atgatagttt cctttatatt
     5461 caatccacaa ttgctattgt tatccgacgt agcgtaaacc gtccacgata tccgttttaa
     5521 tcgcctgaac aacggggata atacccgcga ggatacctac gatcactgca aacgagaatt
     5581 cctgaatcac cgtaatatcc gctaaaataa aaacagggaa gaagtttttt ggaaccgcaa
     5641 cggaaaaggc gccgacaaag aacaacgtga ccgcataacc ggctataccg ccgagaatgc
     5701 cgataataat tgattcgccg gtcacgacgc cgaaaatgtg tttgcctgta aatccgagtg
     5761 ttttcattac agcatattcg cggttacgtt cgcgtgccga catgatcatc gtattggcta
     5821 acaccaaaag gatgatccct atgatcatga acgccataat attgatgccg ttgatgatgg
     5881 cgccgtacgc accgataaaa ccttgattga aagcacgttc ggtttctgtt ttggtttccg
     5941 cgttggaatt tttgaaaatc gcatcaatcg cttcggatac cgcggccgat tcactaggat
     6001 ctttgatttt gacggcgtac cacccgacgt tgccttcgcg cccaggcata tcttgcttca
     6061 tgcgttcgtc gagatatgtc caatggaaaa acatttgtgt ggcatctatc gcgggtgttt
     6121 taggttgata aatcgctcgt actacaaatt cccatcgccc cggataaata tcaccttcga
     6181 tactcatcac atcgccgatt ttcagattat gctttttggc aatgtcggcg ccgacgacgc
     6241 acgcgttacg ttctttttga aatgcggctt tttcttccgg tgtgacgagg aactcgggat
     6301 atacatcaaa tatcgtttcc ggatcacagg ccatgcgcgg gaagaattcc gatttatctt
     6361 tgtacacgcc ttggaaccaa ttgaaatatc cgactttttc cacgccgggt actttgagta
     6421 tctgatcacg atacgcgtaa ggcaaaggaa aaataaacga tacggcttgc cgtgtgataa
     6481 ggcgatccac ggcggcggct tcgacggcgg cataccatga ggtaatgacc gttcgcaaaa
     6541 ggccgaacgc gcctacggcg atggcgatac cgagtatcgt cagtatcgta cgcagcttgt
     6601 ggcgcaacgc atttcgtaac atcattttga agagcatata tttatgtccg ttttggatgt
     6661 cgaaggttta agtgatttaa ctcaacgtcc ctttatccag gtggcgctgg gttttggctt
     6721 tttcggcagc gtgcggatcg tgcgtcacca tgacgatggt tttacccagc tcttgattga
     6781 ggcgcgtcat gagcgtcatg atttcttcgg ccgacgtgcg atccagatcg ccggtcggtt
     6841 cgtccgctac gatgagcgta ggatcggtga cgatcgcgcg ggcaatagcc acacgctgtt
     6901 gctggccgcc ggaaagctga ttgggatagt gtttcatgcg atcgcctaaa ccgacgatgg
     6961 tgagcacttt ttgaatacgt tcatcccgtt cttttttaga aagattggtc agatgcaacg
     7021 gcagttccac attttcatag gccgtgagga caggcataag attgtaaaac tgaaaaatga
     7081 atcccacgtg ataggaacgc cacttagcta aagccgattc gctgagcgaa gcgacgtccg
     7141 taccgccgac gatgatagat ccttgcgtcg gtttatcaat accggcgatc agattgagca
     7201 gcgtggattt accggaaccg gaaggtccca tcagcgccat gaaatcgccg tcggcaatgg
     7261 aaagattaat gttttgcagc acgggaatac tcatgctgcc gcgttggtac gctttgctta
     7321 cgttttgaat ttctactaat ccgggcataa ttggttctcg ggtttagtga ataatgtatg
     7381 ttttattttt gttctgtcgt tacggcgtcg ccgtcattca gatcggcggc aggattatca
     7441 atgactttgt cgccggccgt gagaccgttt acgatttcgg ttttattccc gttacgctca
     7501 ccggtcatta cggggatcgc gcggattttt ccgctgacca cactcaatgc gactttacga
     7561 ccatttcgtt cgacgacggc ggaggtgttg accatcagtt taggtttggc ttgcatcgcg
     7621 ctgtcgctga tcgcttcggt gagaaaattg actttggcac tcatctcggg taaaacacga
     7681 ttgtcaatat caataaatcg tattttggtc aaaatcgtag ctttggaacg atcggcggtc
     7741 ggaacgactt tccaaacctc accgcggtaa cgcttttccg gaagcgcgtc cagcgtgatt
     7801 tcgcaaggct gtttgatttt tacgcgctcg atgttggatt cggaaacgtc ggcttcgagc
     7861 atcagcgaac tcatatcagc cagtgtcacg acattgccgc gggaattggc tccggccgca
     7921 aacggcgcta cgatttcacc gacgtcggca tttttggtaa gcaccgttgc atcaaacggg
     7981 gcacgaataa tcgtattttc caattccact tccgtcgcat ggatcccggc ttgaagtgcg
     8041 gctacgctgg ctttggctga agtcacgcgc gcctgtgcgg atcggtactc ggcgcgaacg
     8101 atttcaaaat cagcgtcggt gataacattg cttttgagta atgtttcact tcgctcgtag
     8161 ttgcgtttca cgcgttccag atcggcttcg gcttgttcga cctgcgcttg accgacgagc
     8221 aaattggcac gggcttgttc gagtccggct tggatatcat tgttttccaa acgtgcaatg
     8281 acctgacccg attggacgcg gtcgccttct tcgacgccaa gaaatactaa acgaccggtg
     8341 cccttggaag cgacggacgc tttgcgttgc ggcacgatat aaccgctggc cgtcagaacg
     8401 ccgccaccgc tgccctgagc tacaaaggcg acggtagtca actgcacgac cggtgttgat
     8461 ttgaacatat tgcgcgacat atagccgata aacccgatgg ctacgacgcc aatgaccatc
     8521 agcgcccatt tgactaaatt accggaggag cgtttgtctt ccggatcaaa tttcattggc
     8581 tcgcgttgaa tacggagcga agataaatct actttttctg cttcagatgc cataattatg
     8641 tttttgtata gacggtgatg ttttattcaa acgaaaccaa agtcagtaaa attacttaaa
     8701 attgcaataa ttcgattcaa atcatatcaa ggcaaatcat aatagttgct tgatatgatc
     8761 atatccggag cgactgatcg gtatttgttt gccatcgcgt aaaattgccg ttcggctgtc
     8821 tttggcgtag agttctatcc gcgcaatacg ttcgacatgg acgatgtacg aacggtgaat
     8881 gcgtacaaaa cgtgacgaat caagttgttt ctctatatcg gacaatgttt gtgttttaag
     8941 ataacacttg ccggtagcgt aaattgcaat gtagtcatcc tgcgcctcga tatagtctat
     9001 ttgtgttacg ggtatcacat gcacttgcac gccctcgcga atgagaatgc gctctgtttt
     9061 tttatccgac gtcaatgacg acggagcttc ccatgtgatc gcgtctcgga tatgttgcag
     9121 tgcatgcgca agcgcggcat caaagcgttc acggctaaac ggcttgagta aataatccaa
     9181 tgcatgcact tcaaaagcct tcaatgcata ttgatcatac gccgtcgtaa aaaccacacc
     9241 gtgctttcgt tgggtcaact ctaaaacttc aaatcccgaa agtttaggca tttggatgtc
     9301 gagaaaaacc aaatcgggat tgatctccgt gattgctttg acggcttcaa acccattggc
     9361 gcattcttcg atcacggaga tatctgtatg cgaagcgaga tattcgcgta tcaactgacg
     9421 ggccaatgct tcatcatcta cgatgataac gcgaagtcgt ggtgtgctca tgctgacgct
     9481 tctccgggcg aaacaggtat tgataattta gcgacaaata ggcggttttg tttttcggtg
     9541 gtgagccggg cgtcgcgacc atattggttt tgaatacgtt tacggatatt acccaaaccg
     9601 atatgtgttc cttcggaata ggatgatcct tcctccattg gattttcgat aatgatatga
     9661 gcatacggtc catcggttaa tgcatgaata cgaatcgtac cgccctcaac gcactgtgcg
     9721 atgccgtgtt tgacggcgtt ttccgctaaa ggttgtatca gcattggcgg taaaagtatg
     9781 tcgttcaatt gctcgggtaa catcatttct acagtcaaac gattgccgaa tcgaactttt
     9841 tcaacagaca aaaagtgttc acacaggttc cattcctcgc gcaaagtgat cagcggtttt
     9901 tgcgcaagcg ccagggtctg acgaaaaaag gcggccagtt ccaccgtcat gcatcgcgca
     9961 ccgtcaggat cgctatgcgt cagagcgctg atggagttga ggctgttgaa taaaaaatgc
    10021 ggatcaattt gcgcacgaag catccgcaac tcggcatcct ttgaaagtac agcgctttgc
    10081 aattcatttt gtttggcttg ttcggcctgc cgcgccatca tcattacata ataaatcgcg
    10141 atggagagta agtacaagac cgtgccgacg acgatcatga tcatagtagt gcctgtatcc
    10201 atgacgatgg ccgtctgcca ttcatgcagg ataacattcc atcccatagc cagcgctaac
    10261 caaaaaaaag cggtgagcat ggcgttgaat aaggccgtga gtaccacacg aagccggcga
    10321 tccgatacga gcggcgagga acggctgaca taatacgccg aaagattgat gaatccgtaa
    10381 atcaaagtga gcggtatcgc aaatgcaaaa gcttgcgtcc cgtctccgcg tccggtaaaa
    10441 tagatcagca tggcgatcaa cgcgcctgca aaaagccaca ccagtatgta ccatgcaagg
    10501 ctgcgaatgt tgccgagtac aggatgcatt agtttcgaac ctcaacgccg cccatgatgg
    10561 catatccttg gatgtgcaag cgtttggtct gatctgcggg atgcagtgtt ttgtcatcaa
    10621 agccgccgag aatcggtgtg ccgttgagaa ataccgccca gtccgtcggn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtctgtggag aatcatgatc gtaccgacgc
    10801 acatcaatac gctgccgaca aaataactcg gtacggtacg cgattggcga attttgagga
    10861 cgccgaaaat aataaagata accggccata aacgcgaaag cctgaagtct tctataattc
    10921 ggaggttgtc caggagaaat aaaacgccga gaacgatggc gataactccg aggataacct
    10981 gcccggggat tttagagtcc gttttcataa tgtgtcgtag tttgtggtgt aaaataatat
    11041 ttgtttaata aacttctggt gattttactg acgccccagg cgatcaataa aaacggccaa
    11101 ctgttgcgat aggtcaatcc ccatgcataa aactgtaccg cgagtaacca gccgccggtt
    11161 aatatccaaa acacgccatc ggcgatgcga cgcgctttgt tgtaactgag taaatcgctg
    11221 ataccgccta cgatcaaaaa tacaggccag aatttccaca acggtccgat gtcatacacg
    11281 tgcaatcggt ctaataacac gacgaaaccg attgcgatca gggcaacgcc ccagattagg
    11341 tgccttttta tgtcatgaga gtgatgcgtg aacatggctt tttccttgtt tttgtttacg
    11401 ccataaacat acataggaaa acaaattttg ttgcacgcat ttcggtgaat ggcggcgggg
    11461 agtcggtgaa cggcgacgcg atcaagtata tgttgcaaaa aagcgcatat aaatggatgt
    11521 ctttttttta gaaaggcaaa tatgcgccct catgcagcgg tattatttca cacaatgctt
    11581 ttaagcgttc aagttattct ggagaaaccg cagaacctga tccattgcaa tacgttcctg
    11641 cctcatgctg tcgcggtgac ggacggtgac ggtttgattt tgcagcgtct cgctatcgat
    11701 cgtaaagcaa tacggcgtac cgatttcatc ttgccggcgg tagcgttttc cgacggtggc
    11761 ggattcatcg taaaatatct tataatgctt cttgatttca ttatataaag tcatagaaac
    11821 ttccggcata ccgtcttttt tgaccaaagg caaaatagcg gctttgaccg gtgcgagcgt
    11881 ggggtgaaac ttcatcacga cgcgggtttc cgttttaccg tcgccggtag gtgcttcttc
    11941 ttcggcatac gcattgacca gcgaagccat gacaccgcgc gaagcgccag ccgatgtttc
    12001 gatcacatac ggcaggtatt tttctttggt cgtatcgtca aaataatcca gatttttacc
    12061 gcaatattcc tgatggcgct tgagatcaaa atccgtacgg ttgtgtatac cttcaatttc
    12121 gccccatccg aaggggaaaa gaaattcgat atcaaaagcc gctgccgcat aatgcgccag
    12181 tttttcatgc gcgtgaaatt ttagtttgtc ttccgtaaag ccgagtgaaa ggtaccattg
    12241 tttacggatt tctttccacg tattaaacca ttccatatcc gtaccgggct tcacaaaaaa
    12301 ctgcatttcc atttgttcga attctcgcgt acggaataaa aagtttttgg tgttgatctc
    12361 attgcggaag gctttgccga tctgtgcgat accaaacggt actttttggc gggaagaatc
    12421 tttgacattg tgaaaattga catagatgcc ttgcgccgtt tccgggcgaa ggtaaactac
    12481 cgcgctggag tcttcgagcg gtccgatgta ggtcttgaac atcagattaa attgtcgcgc
    12541 ttcggtaaac tttccttcgg ccatgcattt atgtgctttc cggccttgat aggctttatt
    12601 gccgcacatc gaatcctcga tctggtcttc acgaaaacgc gctttacatt ctttgcaatc
    12661 caccatcgga tcggtaaagt tttccacgtg accggaggct tcccatacgc gcgggtgcat
    12721 gagtattgag gcatccaaac cttcgatatc gtcccgaaag gtcatagcct tccaccaagc
    12781 atctttataa ttcttcagaa gttcgacgcc gagtgggccg taatcccagc atccgttgag
    12841 gccgccgtaa atttccgatg attgaaaaat aaacccacgc cgcttgcata gcgacacgat
    12901 tttgtccatg agttctgttc ccgaaagagg cttctgcgtg ctcatactgc tccgttgacc
    12961 aacgtgaatc cgcacatagg atgtgtgcgg acataattaa ttttaacgac ggattatatt
    13021 gacattcacg atgatttgca atttgaaact acaaccgatg aataggcaaa aaaatttgac
    13081 agggaacttt ttgaattgaa aacgtatacc atgcacataa aacagattta aaaaccgggt
    13141 tgttatgcga atgacatttt ttgaaaactt ccgtagcgcc gtttcgttta caatgcataa
    13201 tgtgaaggcg cgtttttttc atacttttct ttcagttttg ggaatcgtga tcggtgtggc
    13261 gtccttggtg atggtgctgg ccctcatcga aggtatggag gcatccgttc gtaaacagat
    13321 actgcaaact acgtccatga acatggtcat cgtgagcagc gagccgttta aaaaatcaga
    13381 cggtgttcgt atgcgtaaaa cggacttggc tatactgtca ccggtcgtat ttgattcgct
    13441 gaaaagttca ctgcgtattt cccatactgc gcaattagtt tccacgtatg gtgcagaggt
    13501 aaaagtagat tcatccaaat gggtaggcgc tcagatcaat tttagttcgg ccatggtttt
    13561 aaggacagat tctttgttgg ccggccgggt attgtcggac gaggatattc acgataaaaa
    13621 gaaatttgct gtcattaatc gctttcttgc ttttcaatct gcgaaagatt cgtctttgat
    13681 tcctgcgtta atcggcaaat cgatacatat cggcgcgcaa acttatacca taatgggtat
    13741 tacagccgca cgtgaaacta ccgccgaagc agtattgccg atttcgttta tggattcagc
    13801 gaagcttacc gagtacccgc cgattggttt gtttgaaaca ccgcagatag aaaacgtgat
    13861 accgctgcaa gagcatatac aaaattatct ccatacgcac cctgtcagta cgggagatac
    13921 attgatcgta gccagtaata aatcacgggt tgatcagatg gccaaagggt ttcttgtatt
    13981 tcggattgtg atggggttta tcgtcggcat ttcagtgctt gtcggcggta tcggtgtgat
    14041 gaatgtactg ttaatctcag tcacgcaacg tacgatggaa atcggtgttc gtaaagcggc
    14101 cggtgccaaa cgtcgtgata tattggttca gtttttatcg gaatcggtag tggtatcgtt
    14161 tttggggagc g
//
LOCUS       DAOQGF010000090        13965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2023_length_13965_cov_7.171675, whole genome shotgun sequence.
ACCESSION   DAOQGF010000090 DAOQGF010000000
VERSION     DAOQGF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2023_length_13965_cov_7.171675"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK937_11750"
     CDS             <1..504
                     /locus_tag="PK937_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH33289.1"
                     /translation="VFKMVSNPSDQQEICQEVFTKVYENLRTFQFNAKLSTWIARIAY
                     NTTLNHIAKKKTPLYEDYLTSPAEEENYASADQNLSESLWDEPDMPDAVTQNRETARV
                     LKEAIQSLAPQYRTIVTLFHMDDMSYAEIGEIMQMPEGTVKSYLFRARKMLKARLLEN
                     FSTEELI"
     gene            501..1001
                     /locus_tag="PK937_11755"
     CDS             501..1001
                     /locus_tag="PK937_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33290.1"
                     /translation="MNHLSDAMMQAYLDGTAGITKQNMETHLSVCEECRESMAIYRMM
                     HHTIRQEEFILPAHFADIVAEKVGLVSADESETSVLREWWWLLPVVAAGIGISAYYLP
                     STMTGAPVERVLQENIRQTQTYFTIAEQMGGWIGSNFQYLIFGLFLVLLFDMIERKFL
                     RLRVQH"
     gene            complement(998..1813)
                     /locus_tag="PK937_11760"
     CDS             complement(998..1813)
                     /locus_tag="PK937_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH33291.1"
                     /translation="MTKTNSAGKPRGILSSIPNPRIRYYRDKPTKTLQSTIEHFWMVR
                     WDFPKNRPYIAETLPHPSVHLVFEKDGSSIGGPVTGKFKRTLNGKGFVFGVKFLPGAF
                     SAFYKPSIVNLANHKVNIESVFGESILQLERSIMRMKRFDAMKKAAESFFTSIPIKHD
                     PRAILAQTIVATVMIERDIVHVRQLLARFHITARSLERLFENNIGVTPKWVIQRYRLH
                     EAVEMIKSNAEISLTKLALDLGYHDQAHFIRDFKSLIGMTPSRFLRNATGKTV"
     gene            1902..2342
                     /locus_tag="PK937_11765"
     CDS             1902..2342
                     /locus_tag="PK937_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH33292.1"
                     /translation="MSAKTITSEITIYAQAERVWAVLTEPSLTRQYMFDCEAQSDWKP
                     GSRLEWKSAKDGVVYVRGHIVRNEYPRILEYTVFDPFGGYADIPANYLTVSITLSEKE
                     SATDVSVVQGDFSTVANGEKRYQDSHGGWSYILEKIKTLAEAGT"
     gene            2416..3204
                     /locus_tag="PK937_11770"
     CDS             2416..3204
                     /locus_tag="PK937_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNH33293.1"
                     /translation="MSSEIRELLQAATHAAYEAGKLTLRYYQTASLDVIAKPDASPVT
                     IADRSAEEWLRAFISKEFPGHGILGEEFGETIGSDRYRWILDPIDGTKSFIHGVPFYG
                     TMVAIEDVQKKDAIVGVIHFPALGEILSAATNEGATINGRRVKARTTEYLAEATLLTT
                     DMKSSHQGDYAPILQTLIKETKIVRTWGDCYGHALVACGRADIMVDPKLNLWDIAALK
                     PIIEESGAVIFDRNGVTSMYIDNAIACGPALQKEIWTKLNISHV"
     gene            3335..4696
                     /locus_tag="PK937_11775"
     CDS             3335..4696
                     /locus_tag="PK937_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNH33294.1"
                     /translation="MSFEIQEILETIRMTEVEHFDIRTTTMGISLRDCGRGNASATIQ
                     AIYDKICRYAERHVATAREVEQKYGIAIANKRISITPASIACDGFTRSEFVQLAKKLD
                     EAAERVGVDFLAGFSALVQKGFTKGDRALIESIPEAIGSTKRVCSSINVGSTKAGINM
                     DAIIAVSKVIKDLAQVTASEGAIGCAKFVVFCNAVEDNPFVAGAFHGVTEAECTINVG
                     ISGPGVVLHAIKQAPDADLGELSEIIKRMTFKITRAGELIGREVARQQNIDFGIVDLS
                     LAPTPAPGDSIADILVAMGLEDVGACGTTAALAMLNDAVKKGGLMASSYVGGLSGAFI
                     PVSEDQGMIAAVEKGNLSLEKLEAMTCVCSVGLDMVAVPGDTTWETIAGLIADESAIG
                     MINSKTTAVRIIPVPGKKVGESVDYGGLLGKAPIMPIRNLSPKKFIGRGGRIPAPTVA
                     LSN"
     gene            4715..5368
                     /locus_tag="PK937_11780"
     CDS             4715..5368
                     /locus_tag="PK937_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH33295.1"
                     /translation="MELIAQFIDFFIHLDVHLGELIQNYGTWTYLILFLIIFAETGLV
                     VTPILPGDSLLFAAGAFAGLGYLDPWWLFILLTVAGIIGDSVNYAVGLRIGPKIFHKE
                     NVRFLNKEYLQQAHMFYEKHGGKTIIFARFIPIIRTFAPFVAGIGQMTYRHFIFYNII
                     GSIAWVGLFVSSGYFFGNIPTVRKNFTLVIVAIIIISVIPAVIEFIRARRKSGINEA"
     gene            5420..6565
                     /locus_tag="PK937_11785"
     CDS             5420..6565
                     /locus_tag="PK937_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNH33296.1"
                     /translation="MKSSRSKNSPVTLLIGTRKGAFTLKSDARRRTWKVSAPIFLGHI
                     VHHLMQDPRESKCWLMACKTGHLGPTVFHSDDRGKTWKESKQPPAFHKTDSAKRPRAV
                     DHVFWLTPGHASEPGVWYAGTSPQGLFRSEDHGVTWKNVDGFNENPMYEKWTGGDKDG
                     TPDGPKLHSIIVDPRNARHMYLGCSSGGVFETADQGKTWTPLNKGCDAEFLPQPDPEY
                     GHDPHCVQLHPLMPDRLYQQNHCGVYRMDRTEGRWYRIGKNLPKHIGDIGFSITLHPT
                     DPDVAWVFPMDGTSVWPRTSPGGKPAVYATYNAGKTWKRLDKGFPKKDAYWTVFRQAM
                     TCDQKSKVGLYLGTTSGEVWGSTDEGRSWSCLVRHLPEIFSLEVLTR"
     gene            6572..6829
                     /locus_tag="PK937_11790"
     CDS             6572..6829
                     /locus_tag="PK937_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNH33297.1"
                     /translation="MVIHIPNPLRSYTSDEKVQAEGHTIVEVLKNLDAQFPGIRFRVI
                     DEQDRIRRHIKIFLNEEMVDNLKEPVESKDTLHIICALSGG"
     gene            6887..9043
                     /locus_tag="PK937_11795"
     CDS             6887..9043
                     /locus_tag="PK937_11795"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNH33298.1"
                     /translation="MRQLFLWQCITLCAFVLHIACSDLKAQSYLPVIELTEQSTVMDV
                     TTKMEIFEDAGGQNQIEDVIRDNVVFKINDQAMPNFGFTKSAYWVRFVVRSSSEAHWF
                     LHLDFPLMDELVIYRLSNGLPEPIRITGRMREFSSRDIPHRTFAVHLPLHAGSTDTLY
                     ARFRTDDTMTLPLYLIRADIFRERDHTQQFGFGLYYGIMLVMALYNIFIFFKLRDRSY
                     LYYVLYIVSFGMLLMYQNGISYELFPWAQWRMDPFWSSMASLWGIQFSRHYLLSHRFT
                     PRLDRVLKILLMMSVVSVVISVVAPYRFSIWPGVVIGILIFPLMISTAIIAIRKNYTP
                     GKIFLTAWIVLLTGIVLNAFMSMGLLPANDFTLYGIQVGSALETVLLSLGLANRISVL
                     KHEKEEAEKKLVETELRMKAAVVQQLEEKVRERTALLEAQKQELAQTLEVLKNTEVQL
                     VQTAKMSALGQLIAGVAHEINNPITFIYSNLYPLEDYIEQFKQLAKAAIEHGLHTQLQ
                     DEGVNKKLNNLDELFDDSDRILKSFRLGTQRIRNIVQDLQKFEHADESEKIPVDMNDE
                     IRSVLKLITPQYRDQIEINTQWGPIPEFYCTPSHFHQIFMDIFINAAQAIIQRNKDDK
                     RPKGNLHVATYVDESQAWIGIDIRDDGTGIAQEHITKIFDPFFTTKPVGEGTGLGLAI
                     AYRLIEKNRGTIQVQSKVRDFTQFTIRLPINSLIKE"
     gene            9045..9959
                     /locus_tag="PK937_11800"
     CDS             9045..9959
                     /locus_tag="PK937_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33299.1"
                     /translation="MKRYRWLVITGSVILSLAACEVMVRYTFLPLSSLHNGAVNVPDA
                     RGYALRPGASVEFRGIHEPLPQPVQWIVNKQGLRADRTDSVPRISAKKRIAVFGDSEV
                     FGWSVNYENTFESVMMHEDPSLEVLNFGVPGYNAENIADHVERMAPGFSLDGMIYLIN
                     PNDLDPPLAYREATIDSELLRRIAFAYMSLTETDYVEHRKSDSVRIGFLKQVDRIVQY
                     TQTHHIPLMLVCMNEDELRLLSDKRPLTREAAVTAISVYDVFTQSPKWDVHLSASGHN
                     TLGQRMLSEWRNFASGLPMKDSKTLANN"
     gene            10080..11738
                     /locus_tag="PK937_11805"
     CDS             10080..11738
                     /locus_tag="PK937_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNH33300.1"
                     /translation="MRTHRFVLKTILMALCASSYVFAQSKVKLQYNDLQNILTELRSL
                     ERIETPEGVPDHSASSVAYSLERLSALRLRYSKIDTSGWPIEQRIDHALFGAEINGLD
                     FHLRVLRPWSRDPAYYALIWMEESDTPAHEGPLCHASIDVWRYTFPLSSTDEKKLTRQ
                     LQVIPPLLRQAQSNLTENARDLWIAGTAQVREQISFLDEIKSQSQTAGAELHTAISNA
                     TGAYRDFVSWLEKEAPSKNGPSGIGKENYTWYLRHVLMVPLSWEEEVTLLKRELDRAY
                     ASLALERQRNRDLPEIIPFADAASFQIGTDAAVKKLIRFLEEKKILHVKPYMEKELRK
                     HTGRFQPESQRHFFANIVHRAPNVLYSHLTHWFETAWLRDEPHASAFRKTALPYNIWV
                     SRSEGMATGMEELLMHAGLYDDEPRARELVWIMLAQRCARGLASLYAHANMIDYDAAR
                     QFQVTWTPQGWTGDPALVGFEQHLYLRMPGYGTSYVSGKYMLERLMMERSRQVGSSYS
                     VYQYFTELYSAGMIPVSLIRWQLTGLDDEVRGLMMISNEKNQGK"
     gene            complement(11748..13361)
                     /locus_tag="PK937_11810"
     CDS             complement(11748..13361)
                     /locus_tag="PK937_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33301.1"
                     /translation="MSEPAATISSGERILVVDDSKVQLFAMSTMLKKEGFDVESTHEP
                     LTAKNMIEQKGYDLLLCDLIMPDMDGIGLLRVSKSKRPTMPVVIMTAFGDRESAIQAL
                     KEGAEDYIFKASGDRERDEFIIRIRRALERAKLQKKIFDYQTHLEQMVDERTQELKAT
                     QDQLVQSERLRSLGVITSGVAHDFNNILAVILGRSQLLSQKIHDTDVSKDLDMITRSA
                     LKGSATIKRMQDYTRIRKDERFESIALNSMLEEVIEMTKTRWQDESNSLGVTINLQSQ
                     FGDVPLVSGDASELKDVFINIIFNSVDAMREGGTLSIRTYTEQIENTLWVVTELSDTG
                     CGIPENIQSKVFDPFFTTKNQHGSGLGMSTAYGIVQRHRGHLSFKSKPGEGTTFYVKL
                     MAALFVQQLAPDVARAKEVGPPKPLSVLVVDDDEAVRTTLVEMLEIQDHEVHQAGSGK
                     EALRMIEERSFDIVFTDLGMPGMSGWEVSQEIKKRAPQTNIVMITGWGSQLDKEKAEQ
                     TGIQRILPKPVSFDEIVRAVTDFGLPLKS"
     gene            13444..13830
                     /locus_tag="PK937_11815"
     CDS             13444..13830
                     /locus_tag="PK937_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNH33302.1"
                     /translation="MEKQRFSSGAPWEPIVGYSRSVRAGQFIYVSGTTATDENGKLVG
                     KNDAHAQTVQTIINIEKALHKAGANLTDIVRTRIYVVNIERDWEIIGRVHGEFFKNIR
                     PATAMVEVRKLIDPDMLVEIEAEAVV"
     gene            complement(13831..>13965)
                     /gene="fadA"
                     /locus_tag="PK937_11820"
     CDS             complement(13831..>13965)
                     /gene="fadA"
                     /locus_tag="PK937_11820"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017421693.1"
                     /note="FadA; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadB; similar to
                     FadI2J2 complex; functions in beta-oxidation of fatty
                     acids; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNH33303.1"
                     /translation="GCSGARIIGSLTNEMKRRQSRYGLATMCVGLGQGMATVWERITL
                     "
BASE COUNT         3696 a         2992 c         3467 g         3810 t
ORIGIN      
        1 gtatttaaaa tggtatccaa tccatccgat caacaggaga tttgtcagga agtttttacc
       61 aaagtgtatg aaaatctccg gacctttcag tttaatgcta aactttcgac atggattgcc
      121 cgcatcgctt ataatacgac gctaaatcac atcgcgaaga aaaaaacgcc gctgtatgaa
      181 gattatctaa cctcaccggc ggaggaagaa aattacgcat cggccgatca gaatctttcg
      241 gaaagcctgt gggacgaacc cgacatgccg gatgccgtaa cacaaaaccg ggaaacagcg
      301 agagttttaa aagaagcgat ccaatcatta gcgccgcagt atcggaccat cgtaacgctt
      361 tttcatatgg atgacatgtc gtatgcggaa atcggtgaaa tcatgcagat gccggagggt
      421 acggtaaaaa gttacctttt ccgggcgcgc aaaatgctca aagcgcggtt acttgaaaat
      481 tttagtacgg aggaattaat atgaatcatc tcagtgatgc gatgatgcag gcgtatctgg
      541 acgggaccgc cggtataacc aaacaaaaca tggaaacgca tctgtcggta tgcgaggaat
      601 gccgcgagtc tatggccata tatcggatga tgcaccacac catacgtcag gaagaattta
      661 tattaccggc acattttgcg gatatagtgg cggaaaaagt gggtttggtt tccgctgatg
      721 agtcggaaac atctgtttta cgtgagtggt ggtggctgtt gccggtcgtc gcagcgggca
      781 tcggcatttc cgcgtattat ttgccttcta cgatgaccgg agcaccggtt gagcgcgttt
      841 tacaggaaaa tattcggcag acgcaaacct attttacgat agctgaacaa atgggcggtt
      901 ggatcggttc caattttcag tatcttattt ttgggttatt tctcgtattg ctttttgata
      961 tgatcgagcg gaaatttttg agattgaggg tgcagcacta aacagttttg cccgtcgcat
     1021 ttcgtaaaaa acgtgacggt gtcatgccga ttagcgattt aaaatcacga atgaaatgtg
     1081 cctgatcatg atatcccaga tccaaggcca gtttggtcaa tgaaatttct gcgttagatt
     1141 ttatcatttc tacagcctcg tgtaagcggt aacgttgaat cacccacttt ggcgttacgc
     1201 cgatattatt ttcaaacaat ctttcaagag aacgggcggt aatatggaac cgcgctaaaa
     1261 gctggcgcac gtgaacgata tcacgttcaa tcatgaccgt tgctacgatg gtttgtgcaa
     1321 ggatagcacg cgggtcatgt ttgatcggta tggatgtaaa aaatgactca gcggcttttt
     1381 tcattgcatc aaatcgcttc atgcgcatta tggaacgctc taattgcaga atggattcac
     1441 cgaaaacgga ttcgatgttt actttgtgat tggccaggtt aactatagac ggtttataaa
     1501 atgccgaaaa agcgccggga agaaatttaa caccaaaaac aaaaccttta ccattcagag
     1561 ttcgtttaaa ttttccggta accggtccgc caatagagga gccgtctttc tcgaaaacca
     1621 aatgcacaga aggatgcggc agagtttcag ctatatacgg gcggtttttc ggaaaatccc
     1681 atcgtaccat ccaaaaatgt tcgatcgtag attggagcgt tttcgtaggt ttgtcgcggt
     1741 aataacgaat acggggattt ggaatagaac tcagaatgcc tcgcggttta ccggcgctat
     1801 tagtcttcgt catttgtcgt gtttttacaa ttatgatgag tttcttatga gtatattggc
     1861 aacgaacgaa aattgaaaca aagaaattga aggatcaatt tatgtcggct aagacaatta
     1921 cttcagaaat aacgatatat gcccaagccg agcgggtgtg ggcggtgttg actgaaccgt
     1981 cgttgacgcg gcagtacatg tttgactgtg aagcgcaaag cgattggaaa ccaggcagtc
     2041 gtttagaatg gaaaagcgca aaggacggcg tcgtatatgt gcgcggtcat atcgttcgta
     2101 atgaatatcc ccgtattttg gagtacacgg ttttcgaccc atttggagga tatgcggata
     2161 tccctgcaaa ttatctgacg gtgagcatca cacttagtga aaaggaatcc gcaaccgacg
     2221 tgtccgtcgt acaaggggat ttttcgacgg tcgctaacgg tgaaaagcgt tatcaggatt
     2281 cccacggggg ttggtcgtat attttagaaa aaataaaaac gctggccgaa gccggcacgt
     2341 gaatacgttt gattatcatt tcgtttttca gtaaaatcaa cgcgtaaaat cgttcatttt
     2401 tcagcgttgt tctccatgtc ttccgaaatc cgtgagttgc ttcaggcggc tacacatgcc
     2461 gcgtatgaag caggaaaact cacactgcgt tattatcaga cggcctcctt ggatgtgatc
     2521 gccaagccgg acgcctcgcc cgtcacgatc gccgaccgtt ctgcggagga atggttgcgc
     2581 gcattcatca gtaaagaatt tccgggtcat ggaattttgg gagaagaatt cggcgaaact
     2641 atcggtagcg accggtatcg ctggatattg gatcctattg acggcaccaa atcatttatc
     2701 cacggtgtac ctttttacgg gacgatggtt gctatcgaag atgtgcaaaa aaaagatgcc
     2761 atcgtcggtg tgatacattt tccggcactg ggcgaaatcc tttccgcggc tactaatgaa
     2821 ggtgcaacta tcaacggtcg tagagtcaaa gcgcgcacaa cggaatatct ggctgaagcg
     2881 acgcttctca ccacggatat gaaaagttct caccaaggag actacgcacc cattcttcaa
     2941 acactcatca aagaaactaa aatcgttcgc acgtggggcg attgttatgg gcatgctttg
     3001 gtggcgtgcg gtcgggccga tattatggtg gaccctaagc tcaatctttg ggatatcgcc
     3061 gcactcaagc cgatcatcga agaatccggc gctgtgattt ttgatcgaaa tggcgttacg
     3121 tctatgtata ttgacaacgc gattgcgtgc ggaccggcgc tccaaaagga aatatggacg
     3181 aaattgaata tatcccatgt gtaagctctt gaaccgcaaa gcatgagcga aaacgcctcc
     3241 gggtttgatc tcgtcatgac aattcgcatg aattctcagt tgatccgtgt aataaagaaa
     3301 aaatagtttt ctaatgttct tatattgagg tcatatgtca tttgaaattc aagaaatttt
     3361 agaaaccatc cgcatgacgg aggtagagca ttttgatatt cgcacgacga cgatgggtat
     3421 cagtttacga gattgcggac gcggtaatgc gtcggcgacg atccaggcga tctacgataa
     3481 aatctgtcgt tacgcggaac gtcatgtagc gacggcgcgt gaggtcgaac aaaaatacgg
     3541 gatagccatt gccaataaac gtatttccat cacaccggca tccattgctt gtgacggatt
     3601 tacgcgtagt gaatttgttc agttggcgaa aaaattagat gaagcggccg aacgggtcgg
     3661 tgtagatttt ttggccggat tttcagcgct cgtacaaaag ggttttacca aaggtgatcg
     3721 ggcattgatc gaatccattc ccgaagccat aggtagtacc aaacgcgtat gttcgtccat
     3781 caatgtcggt tctaccaaag ccggtatcaa tatggatgcc attattgcgg tatccaaagt
     3841 catcaaagat ttggcgcagg tcacggcgtc cgagggcgcg atcggttgtg ctaagtttgt
     3901 cgtgttttgt aatgcggtcg aagataatcc ttttgtagcc ggggcatttc acggagtgac
     3961 ggaagccgaa tgcacgatca atgtcggaat cagcggaccg ggtgtcgtgc tgcatgcgat
     4021 caaacaagcg ccggatgcgg atttaggcga actctcggaa attatcaaac gtatgacgtt
     4081 taaaattacc cgtgcgggcg aattgatcgg ccgtgaagtg gcgcgtcagc agaatattga
     4141 tttcggtatt gttgatttgt ctttggcacc tacaccggcg ccgggcgaca gtatcgcgga
     4201 tattcttgtt gcgatgggac ttgaagatgt cggcgcatgc ggtacgacag cggcacttgc
     4261 gatgctcaac gatgccgtta aaaaaggcgg tctcatggcg tcgtcgtatg tcggtggttt
     4321 gagcggcgct tttatcccgg tgagcgaaga tcaaggtatg atcgctgccg tagaaaaagg
     4381 aaatttgtcg ttggaaaaat tagaagcaat gacctgtgtg tgttcggtcg gtttggatat
     4441 ggttgccgtg cccggtgata caacgtggga aacaatcgcc ggtctgattg cggacgaatc
     4501 agctatcgga atgatcaaca gcaaaactac ggcggttcgc atcattcccg tacccggaaa
     4561 aaaagtcggc gaatccgttg attatggcgg actgctgggc aaagcgccga tcatgccgat
     4621 tcgtaattta tcacctaaaa aatttatcgg gcgcggaggc cgcattccag cacctacggt
     4681 agccctttca aactaacaaa cgaggatcga tacattggaa ttgattgcgc agtttataga
     4741 tttttttatt catctggatg tccacttagg cgaactcatt cagaattacg gtacatggac
     4801 atatctgatt ttatttctca tcatttttgc agagaccgga ttggtggtca caccgatttt
     4861 accgggtgat tcgcttcttt ttgcggcggg tgcgtttgcc ggcttaggtt atcttgatcc
     4921 gtggtggtta tttattttac tcacggtagc cggtattatc ggtgattcgg tcaattatgc
     4981 cgtgggttta cgcatcgggc cgaaaatttt tcataaagaa aatgtacgtt ttctcaataa
     5041 agaatattta caacaagcgc atatgtttta cgaaaaacac ggcggtaaaa cgattatttt
     5101 tgcgcgcttt attcccatca ttcgcacgtt tgctcctttt gttgccggta ttggtcaaat
     5161 gacgtaccgt cactttattt tctacaacat catcggttct atcgcgtggg tcggactgtt
     5221 cgtttctagc ggttattttt tcggtaatat cccgaccgtg cgaaaaaact ttaccctcgt
     5281 gattgtggcg atcatcatca tatcggtgat tcctgccgtg atcgaattca ttcgtgcccg
     5341 tcgcaagtcc ggtataaacg aagcatagtt ttagctatat attttagaga acgtaaagca
     5401 cgtcgtgcaa ggaggttgta tgaaatcttc acgttcaaaa aattcgcccg taacattgct
     5461 tatcggtacg cgtaagggtg cgtttacgct caaaagcgat gcgcggcgcc gcacgtggaa
     5521 agtatcggca ccgatttttt tggggcatat cgtacatcat ctgatgcagg atccgcgcga
     5581 atccaaatgt tggctgatgg cttgcaaaac aggtcatctc gggcctacgg tatttcactc
     5641 tgatgatcgc ggtaaaacct ggaaagaatc caaacaaccg ccggcgtttc ataaaacaga
     5701 ttcggccaaa cgtccacgtg cggtagacca tgtcttttgg ttgacgccag gtcatgccag
     5761 tgaacccgga gtgtggtatg ccggtacatc gcctcagggt ttgtttcgca gcgaagatca
     5821 tggtgtgacg tggaaaaatg tagacggctt caatgaaaat ccgatgtatg aaaaatggac
     5881 tggtggcgat aaagacggca ccccggacgg ccccaaactt cattcgatta ttgtggatcc
     5941 gcgtaatgca cgtcatatgt atcttggttg ctccagcggt ggtgtttttg aaaccgcaga
     6001 tcaaggcaaa acgtggacac ctttgaataa aggttgtgat gcggaatttt taccgcagcc
     6061 tgatccggag tacggtcacg atccgcactg cgtacaactt catccgctca tgccggaccg
     6121 cttgtatcag cagaatcatt gcggggtgta tcgtatggat cgcacggagg gtcgctggta
     6181 tcgaattggg aaaaacctgc ccaaacatat cggcgatatc ggtttttcga ttacgctgca
     6241 tcctacggat ccggacgtgg cctgggtttt tccgatggac gggacgtcgg tttggccgcg
     6301 tacttcaccc ggcggtaaac cggcagtcta tgcaacgtac aatgcgggaa aaacgtggaa
     6361 acgtttagat aaaggatttc cgaaaaaaga tgcttactgg acggtgtttc gtcaagcgat
     6421 gacatgcgac cagaaaagta aagtcggttt atatctcgga acgacctccg gagaggtgtg
     6481 gggcagtacc gatgaagggc gatcctggtc gtgtttagtt cggcacttgc cggagatttt
     6541 ttcacttgaa gttttaaccc ggtgatgatg tatggtaata cacattccca atcctttgcg
     6601 ttcatataca tcggacgaaa aagttcaggc tgaaggtcat acgatagtgg aagttctaaa
     6661 aaatttggat gcacaatttc cgggaatacg atttcgtgtg atagatgaac aagaccgaat
     6721 acgaaggcac atcaaaattt ttcttaatga agagatggtc gataacctta aagaaccggt
     6781 tgaatccaaa gatacattgc acatcatttg cgcgttaagc ggaggttgac agttctactg
     6841 ttagcccggg cattataaaa cgcatcattt aattttaatc ttactcatgc gacaattatt
     6901 tttatggcag tgcattacgc tatgtgcatt tgttttgcat atcgcatgtt ctgatctaaa
     6961 agcacaaagc tatctgccgg tcattgagtt gacagaacaa tccacggtaa tggatgtgac
     7021 cacaaaaatg gagatttttg aagatgcggg cggacaaaat caaattgagg atgttatccg
     7081 tgataatgtt gtgtttaaaa taaacgatca ggctatgcct aattttggat ttacaaaatc
     7141 cgcttattgg gttcgttttg tcgtgcggtc atcatcggag gcacattggt ttttgcatct
     7201 ggattttcct ctgatggatg aattggtgat atacagatta tcgaacgggt tgccggaacc
     7261 aatccgaata accggaagaa tgcgtgaatt ttcctcgcgc gatattccgc atcgtacgtt
     7321 cgcagttcat ttgcctttgc atgcgggttc gacggatact ttgtacgcac ggtttcgtac
     7381 ggatgatacg atgacgttgc ccttgtatct tattcgggcg gatatttttc gcgaaagaga
     7441 ccatacgcag cagtttggct tcggtttgta ctacggtatt atgctcgtga tggccttgta
     7501 taatattttt atctttttca aacttcgcga tcgatcctat ctctattatg ttttatatat
     7561 tgtcagtttt gggatgctcc tgatgtatca aaacggcatt agctatgaac tttttccgtg
     7621 ggctcaatgg cgaatggatc cgttttggag ttcgatggct agtttgtggg gcattcagtt
     7681 ttcgaggcat tacctgctta gtcatcgttt tacgccgcgc ctggatcgcg tgcttaagat
     7741 tttgttaatg atgtctgtcg tatccgttgt gatttctgtt gtcgcccctt atcggttttc
     7801 gatttggccc ggtgtagtga tcgggatact gattttccct ttaatgattt ctacggcaat
     7861 tatagcgatt cgtaaaaatt atacgcccgg aaagatattt ctcacggcat ggattgtttt
     7921 gcttacaggt attgttctta acgccttcat gagcatggga ttgcttccgg ccaacgattt
     7981 cactctttat ggcattcagg tgggttcagc gttagaaacg gttttacttt cactcggcct
     8041 tgccaaccgt atcagtgtac tcaaacatga aaaagaagaa gctgaaaaaa aattagtcga
     8101 aacagaactt cgtatgaaag ccgctgtcgt tcaacaactt gaagaaaaag tgcgtgaacg
     8161 aacggccctt ttggaagcgc aaaaacaaga actggcgcag acgctagaag ttctcaaaaa
     8221 cacagaagtc caattggtac aaaccgcaaa gatgtcggct ttgggtcagc ttattgccgg
     8281 agtggcacat gagatcaata atccgataac ttttatttac tccaatctgt accctctgga
     8341 agattatatc gaacagttca aacaacttgc caaagcggcg attgagcacg gactgcacac
     8401 gcaattgcaa gatgaaggtg taaacaaaaa actaaacaat ctggatgaat tgtttgatga
     8461 ttcggatcgc atactcaaaa gttttcgttt aggtacgcag cgcatacgaa acatcgtgca
     8521 ggatttacaa aaatttgaac atgcggatga gagtgaaaaa ataccggttg atatgaacga
     8581 tgagatccgc agcgttctga agctcataac accgcaatac cgcgatcaga tagaaatcaa
     8641 cacgcaatgg ggtcctattc ctgagttcta ttgcacgcct tcgcattttc atcagatctt
     8701 tatggatatt tttattaatg cggcccaggc cattatccag cggaataaag acgataaaag
     8761 acctaagggc aatcttcacg ttgctacgta tgtagatgaa agccaggcat ggatcggaat
     8821 agatatccgt gatgacggaa caggtatcgc gcaggagcat attacgaaaa ttttcgatcc
     8881 tttttttacg accaaaccgg tcggcgaagg tacagggttg ggtttggcca tagcgtaccg
     8941 attgatcgaa aaaaaccgcg gcacgattca agtgcaatcc aaagtaaggg actttactca
     9001 atttactatt cgattaccta tcaactcatt aataaaggag tgacgtgaaa cgctatcgtt
     9061 ggctggtgat aacagggtcg gttattttaa gtttggcggc gtgtgaagta atggtgcggt
     9121 acactttttt acctctttca agtctgcaca atggtgctgt caatgttccg gatgcaaggg
     9181 gttatgcgtt gcgtcccggg gcttccgtcg aatttcgcgg tatacacgaa ccgttgccac
     9241 aaccggtgca atggatcgtt aataaacaag gtttgcgggc tgatcgtacg gactcagtgc
     9301 cgcggatctc cgccaaaaaa cgtattgccg tatttggtga cagtgaagta ttcggttggt
     9361 cggtaaatta cgaaaataca tttgaatcgg taatgatgca tgaggatccg tcgctggagg
     9421 tgctcaattt cggtgtgccc ggatacaacg ctgaaaatat agcggatcat gtggagcgaa
     9481 tggcgccggg tttttcgtta gacggtatga tttatttgat caatcccaat gatcttgatc
     9541 cgccattggc ttaccgtgaa gcaacgattg attcggaatt attgcgtcgg attgcatttg
     9601 cctatatgag tctgacggaa acggactatg tagaacaccg aaaatccgac agcgttcgca
     9661 tcggatttct caaacaggta gatcgcattg tgcaatatac tcaaacgcat cacattcctt
     9721 tgatgttagt ttgtatgaat gaagatgagc ttcgtttatt atcggataaa cgacctttga
     9781 cccgggaagc cgcggtgacg gcgatttccg tgtacgatgt atttacccaa tcgccgaaat
     9841 gggatgttca tctttcagcg agcggtcata atacattggg tcaacgcatg cttagcgaat
     9901 ggcgaaattt tgcatccggc ttaccgatga aagattcaaa gacattagcg aacaactaaa
     9961 aacagaatcg aatggttttt agtgccatgt gtggaacatg ttttattatt tctttgttct
    10021 caagtatacg ataagttttt tgtatatata acatttcaac tatcagggat gtgaaattta
    10081 tgagaacaca tcggtttgtt ttaaagacta tactcatggc gttgtgcgcg tcatcctacg
    10141 tttttgccca atctaaagtc aagttacaat acaacgatct tcaaaatatt cttacggaac
    10201 ttcgatcgct ggagcgtatc gaaacaccgg agggtgtgcc ggaccattcc gcttcgtcgg
    10261 tagcgtattc tttggagcga ttgagtgcgt tgcgtctgcg gtattcgaaa atagatacgt
    10321 ccgggtggcc gatcgaacag cgtattgatc atgccttgtt cggtgccgaa atcaacggcc
    10381 tggattttca tctgcgggtt cttcgtccct ggtcacgcga cccggcgtat tatgcattga
    10441 tctggatgga agaaagtgat acaccggctc acgaaggccc gctgtgtcat gcgtcgattg
    10501 atgtgtggcg ttacactttt ccactgtcgt caacggatga aaaaaaactc acccgccaac
    10561 tgcaagtcat cccaccgctg ctacggcagg cccaatctaa cctgaccgaa aatgcgcgcg
    10621 atctctggat cgccggtacg gcacaagtac gcgaacaaat cagctttctg gatgaaatca
    10681 aatcgcaatc gcaaaccgcc ggtgcggaac tccataccgc catcagcaat gctaccggtg
    10741 catatcgtga ttttgtatca tggctcgaaa aagaagcacc ctctaaaaac ggaccatcgg
    10801 gcatcggtaa agaaaattat acgtggtatt tgcgccacgt tttgatggtg ccgctgtcgt
    10861 gggaggaaga ggtgacacta ctcaaacgtg aattggatcg ggcgtatgct tcgctggctt
    10921 tggaacggca gcgtaatcgc gatcttccgg agattattcc ttttgcggat gcggcgtcgt
    10981 ttcaaatagg taccgatgcg gccgtaaaaa aattaatacg ctttctggaa gagaagaaaa
    11041 tattgcatgt aaaaccgtac atggaaaaag aattgcgtaa acatacggga cgattccaac
    11101 ctgaatcgca acgtcatttt tttgcaaata tcgttcatcg tgcaccgaat gttttatatt
    11161 cgcatctcac ccattggttt gagacagcat ggcttcgtga tgagccgcat gccagcgcat
    11221 ttcgtaaaac ggcactgcct tacaatatct gggttagccg ttccgaaggc atggctacgg
    11281 gaatggaaga acttctgatg catgctggtt tgtacgacga tgagccgcgt gcccgtgaac
    11341 tggtgtggat catgctggcc caacgttgtg cgcggggctt ggcttcgctg tatgcccatg
    11401 ccaatatgat agactacgat gccgcacggc aatttcaggt aacgtggacg ccgcaaggct
    11461 ggacgggtga tcccgcattg gtaggatttg aacagcatct gtatctgcgc atgccgggtt
    11521 atggtacaag ttatgtaagc ggaaagtata tgttggaacg ccttatgatg gagcgcagtc
    11581 gtcaggtcgg ttcatcctat agtgtctatc agtattttac agaactgtac tccgccggta
    11641 tgatccccgt gtcattgata cgatggcaat tgacaggatt agatgatgaa gtgcgtggtc
    11701 tgatgatgat atcaaacgaa aaaaatcagg ggaaatgatt tttataatta gctttttaga
    11761 ggcaaaccga aatcggttac tgcgcgaacg atctcgtcaa atgaaacagg tttaggtagg
    11821 atacgctgaa tgcccgtttg ttctgctttt tctttatcga gttgagaacc ccagccggtg
    11881 atcataacga tattcgtttg tggtgcgcgt ttcttaattt cttgcgaaac ttcccagccg
    11941 gacatacccg gcatacccaa gtcagtaaaa acaatatcaa aactgcgttc ttcgatcata
    12001 cgaagggctt ctttaccgga accggcttgg tgtacttcgt ggtcttgtat ctcaagcatt
    12061 tcaaccaacg tagtgcgcac agcttcgtca tcatctacta caagtacaga tagaggtttt
    12121 ggcgggccga cttctttagc tcgcgcgaca tccggtgcaa gttgctgtac aaaaagagcc
    12181 gccatgagct tgacataaaa tgtcgtacct tcacccggtt tgcttttaaa cgaaagatgc
    12241 ccgcggtgcc gctgcacgat accgtaagcg gtgctcatac ccaagccgct gccatgctga
    12301 tttttggtcg taaagaaagg atcaaaaact ttggattgaa tattttcagg aatcccgcag
    12361 cccgtatcgc ttaattccgt cacgacccac aacgtgtttt cgatctgttc ggtgtaggta
    12421 cgaatcgaaa gcgtaccgcc ttcgcgcatt gcgtcaacgg aattgaaaat aatattaata
    12481 aaaacgtctt tgagttccga agcgtcgcct gaaacgagcg gtacatcgcc gaattgactt
    12541 tgcaaattga tcgtaacacc caaactgttg gattcatcct gccaacgtgt tttggtcatt
    12601 tcgatcactt cttccagcat gctattgagc gctatggatt cgaagcgttc atctttacgg
    12661 atgcgcgtgt aatcctgcat gcgtttgatc gtggccgatc ctttgagcgc cgatcgcgta
    12721 atcatatcca gatctttgga cacatccgta tcgtggattt tctgagaaag cagttgcgaa
    12781 cgtcccaata tgacggcgag gatattatta aaatcgtgag cgacaccact ggtgatgaca
    12841 cccaacgaac gcaaacgttc ggactgaacc aactggtcct gcgtcgcttt cagttcttgc
    12901 gtccgctcgt ccaccatttg ttcaaggtga gtctggtagt caaatatttt tttctgaagt
    12961 ttggcgcgtt caagagcacg tcggatacgg atgataaatt cgtcccgctc gcggtcaccg
    13021 ctggctttaa aaatataatc ttccgcgcct tctttgagcg cctggatggc tgattcacga
    13081 tcgccgaatg ccgtcataat cacgaccggc atggtggggc gtttggattt ggaaacgcgc
    13141 agtaatccga taccgtccat atcgggcatg ataagatcgc acagcaaaag gtcgtaacct
    13201 ttttgctcga tcatgttttt tgcggtaagc ggttcgtgcg tactctcgac atcaaagcct
    13261 tcttttttga gcattgtgct catcgcaaaa agctgaactt tgctgtcgtc gactaccaag
    13321 atacgctcgc cggatgagat agtggcggct ggttcactca tagcgcatgt aatgtataaa
    13381 aaacatactg gaatagaaaa taaaaaaatt gttctttgtc tcatacaaga tcaaggaaac
    13441 actatggaaa aacaacgctt cagttcaggt gcaccatggg aaccgattgt cggctattca
    13501 cgatccgttc gcgcaggtca attcatctat gtatcaggaa cgaccgcgac ggatgaaaac
    13561 ggtaagttgg tcggaaaaaa tgacgcgcac gctcaaactg tgcaaacgat cattaatatc
    13621 gaaaaagcat tgcacaaagc cggcgccaat cttaccgata tcgtgcgaac acgtatctat
    13681 gttgtcaata tcgagcgcga ttgggaaatc atcgggcggg tgcatggcga attttttaaa
    13741 aacattcgcc cggctacagc gatggttgaa gtgcggaaat tgatagatcc ggatatgttg
    13801 gtggaaatcg aagcggaagc ggtagtctga tcaaagcgta atgcgctccc aaaccgtagc
    13861 catgccttgc cccagaccga cgcacatcgt tgccaatccg taacgcgatt gacggcgttt
    13921 catctcgttg gtaagtgaac cgatgatgcg tgcgcccgaa cagcc
//
LOCUS       DAOQGF010000091        13920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2039_length_13920_cov_6.171006, whole genome shotgun sequence.
ACCESSION   DAOQGF010000091 DAOQGF010000000
VERSION     DAOQGF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13920
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_13920_cov_6.171006"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="folK"
                     /locus_tag="PK937_11825"
     CDS             complement(<1..531)
                     /gene="folK"
                     /locus_tag="PK937_11825"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNH33304.1"
                     /translation="MKNKVLLSLGSNIGDRMQHLREAVDLISRFAGIKKMASIYETEP
                     VSPIMQDNYYNTAMIIDTDLDAQSLMKKLLDVEKSLGRERTIPGNARTIDLDIILFDD
                     LIMNTPDTVIPHPRFAERNFVLVPSVEIAGNWLCPIRHLTIQEILNLSRDTHHVQYAV
                     SRDNPDVIFHLLPMGDA"
     gene            complement(528..902)
                     /gene="folB"
                     /locus_tag="PK937_11830"
     CDS             complement(528..902)
                     /gene="folB"
                     /locus_tag="PK937_11830"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNH33305.1"
                     /translation="MKNNDKIRLVNLIIYAHHGAHEEERRLGQRFEIDVELGLDFQPA
                     ALADDLHLSVDYGTVYQRIHTVVTEKKYYLIEAVAENIAQRLLNDFPIQVVTVCVRKP
                     SVPIHGSLDHVEVEITRERKTA"
     gene            complement(904..2070)
                     /locus_tag="PK937_11835"
     CDS             complement(904..2070)
                     /locus_tag="PK937_11835"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNH33306.1"
                     /translation="MTFRLADRFEELPVHAFAEVDRLKRESIAQGKDVIDLGVGDPDI
                     PTPQPIIDACTQALQKPANHRYPYQAGSMRFRESVAAFMKRRYDVTVDTQRNIVPLIG
                     SKEGIAHFPIAFVNPGEIVIAPDPGYPAYVTGTKLAGGVFHSIPLLAKNRFLMPLNEI
                     PADVVKRARLMFFNYPNNPTTGWADQCDFESIAQFCKTNDIIAAHDAAYQELFFEEAP
                     PSLMQTTCGLDGGIEFHSLSKTFNMTGWRVGFAVGEPSLIAGLSRLKANIDTGLFLAL
                     QEAAAFALDHCEEFTKPMIELYRRRKDTVVQGLNAAGFKSHHGKATIYVWTEVPGGSD
                     SMIFTKQLLDKTGVVVTPGSALGQSSGSYFRIALTQSEERLSEAVRRMAAFAKT"
     gene            complement(2207..4273)
                     /locus_tag="PK937_11840"
     CDS             complement(2207..4273)
                     /locus_tag="PK937_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNH33307.1"
                     /translation="MKLFTKTLTAVCLLMLSWTFPGMAQNEMIDEDGVVADVRAKYLA
                     DSKSVLIYNGNREIPEGRTIKSDILLLNGDLIIAGTVEGKIVVSNGNIIMRETGSVTR
                     DAVSINGRVETVNSDQIAGNILQVKSSQRSMNKQDIAAKQESNFTTDESTADETSSEV
                     TAYAPSVKSKSYQENDPEELTDDETSVTPPTVQRKDGARVYDYKNKRKNKSITPYIEV
                     PPVVEAPNADNMTEADWKRFNREMEKFNRKMEKFNRKMARMNETVEKDLPEYEYNYTP
                     QTDEDDNEDEDRSHSASFVRVDNVPRRNEDRRSPRYYSRNNYWDNHQEKLRNTSFVLF
                     DYNRVDGLYIGGKIDRDHKIYDNKPFQIYGEAGYAFGPKDFHYRLGLDKIWGSEYRFT
                     LGGEVHDLTTTNDNWIVGQMENAMNSMLLKIDNRDYFRTRGFSFQAQQNLNHQFKISA
                     AYKVDDYSSASNNVNWALFRSKKIDFRTNPMIDEGRMNSVVVRAELNTRGWVYNNRKY
                     SKRVGWDIYGEAERSRDEWNSDFDFSRYVLSIARYQPLSRYENLDMRLMLGSVTGTVP
                     AQKMFHLGGISTLRGHDYKAMSGNQMALANVEYRISSGALKNDRIFPIHPFSAILFAD
                     AGYAWNNTIYAIRELARGTNLRDIQTDLGVGIGDENDLFRFDIAKSVSEKGSPYKFAF
                     RVNYIF"
     gene            complement(4413..5018)
                     /locus_tag="PK937_11845"
     CDS             complement(4413..5018)
                     /locus_tag="PK937_11845"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNH33308.1"
                     /translation="MHDFTGIILAGGLSQRMGQDKFLLDFYGTPLLKNLADELAKTAR
                     EIHIVTKSSDSLAALNLHHSIITDVHPSRSAMAGIYTGLQSALYDHVFVLACDLPLFD
                     HRVTTLMQSRLPQFDTIAPKTPGGLETLCAFYHKKHLPIIDSMIRDENYRLTDFLNQT
                     KNLVLPASHFESAIYPDVFFNMNSPEDYSTALKLFKDRKSI"
     gene            complement(5021..5926)
                     /locus_tag="PK937_11850"
     CDS             complement(5021..5926)
                     /locus_tag="PK937_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33309.1"
                     /translation="MVNPTDRQWLDENEIIDKFVFGRLTDNESIRLNELRKNEDFNAM
                     FDRELMTIAALRAHGRIAMKQRIRELLHTSSPLHLSHTANPAPGKVMTMRFVWRAAAT
                     VAVIAGGLWIYKMNLQPAPEVAKKQNIQNEQAPIVNTDKQAPAQTSTEPGTPSNDVAA
                     KRTNKPNERKSMIAGQLPPSKTDRTRPAALAAVPHEVYVSISVLNATESIETRILFKN
                     PFDLVAADIRETQEEKDGQLNWFYVYYENRIVSAYLDNSKYVARFIGTHLKEEPGVLI
                     INTPQSKYRVDLTTKEKFKKAVRIP"
     gene            complement(5980..6564)
                     /locus_tag="PK937_11855"
     CDS             complement(5980..6564)
                     /locus_tag="PK937_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH33310.1"
                     /translation="MNSGSPVYENATIETDQILIERIRRGDEKALVTIYHNNIMMVRK
                     YVMDNSGHEDDAQDMLQDAIVVLWENVTKSQFDLTSKISTYLYSVVRNKWLREINKRK
                     IGKTSDIADYEMAEEQIDALRSVVQKEEFKTILRCIENMGATCRKLLMMFYLEEKGME
                     EIAHELKFSNTDVAKAKKWQCKKELEKMVEKAFQ"
     gene            complement(6630..7433)
                     /locus_tag="PK937_11860"
     CDS             complement(6630..7433)
                     /locus_tag="PK937_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33311.1"
                     /translation="MKKLTSWFAILLIATVIKSCGGCNERQLLEKAKPYYEKAVELEQ
                     NGQFSSAVAFYDTAIQIFPDYRDAYFQMGTVYERLVLPEKAAASYRAAIRIDADFAAA
                     HNNLGNCYGKLNKLDSALVHLKKAIQLDPQSPSAHYNLGHAALLKGDFSGAETAFTAA
                     LALRPSDPDYLQALAMLYVTQNKLAEALPLYERAALQRLAQPELRYRMALVYRDQKMY
                     PSALTELEAYLKTRADPQEQTMIRRLIGEIKVEQSREKLAQARKVYQKP"
     gene            complement(7525..9531)
                     /gene="uvrB"
                     /locus_tag="PK937_11865"
     CDS             complement(7525..9531)
                     /gene="uvrB"
                     /locus_tag="PK937_11865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNH33312.1"
                     /translation="MEFKLKSDYALQGDQKEAVAHLVRGIRDGQQHQTLLGVTGSGKT
                     FTMANVIAQIGKPTLVLSHNKTLAAQLYGELKQFFPDNAVEYFISYYDYYQPEAYVAS
                     TDTYIAKDMSINDEIEKLRLRATSSLLERQDVIIVASVSCIYGIGSPQDYKDMFVFTE
                     KGQTVDRNKMLARLVDMQYNRNDIAFERGTFRVRGDVIDIYPAYEDHYVRIELFGDEI
                     EQIAYLDPVTHKIITKPATAIIFPATHFVTSESKLHQAILRIQKELEERLTFFRSQNR
                     LVEAQRLEQRTKYDIEMMLELGYCSGIENYSRHIDGRGEGDRPACLLDYFPKDFLMFI
                     DESHVTVSQVRAMYNGDRARKTNLVEYGFRLPSALDNRPLKFEEFEKMLSQVVYVSAT
                     PADYELQKSGGEIVEQIIRPTGLLDPMIEVRPVKNQIDDLIHEIRKRTQVGERVLVTT
                     LTKRMAEDLTEYLANLKIRVRYMHSEIDSLERVDILRDLRLAGFDVLVGINLLREGLD
                     LPEVSLVAVLDADKEGFLRSERSLMQTAGRAARNANGTVIFYADKMTDSMKLVIDETG
                     RRRAMQMAHNEKHGITPTTVFKSKEQIIRSTSVADYFTYKEGQEEAQALAASEATIDY
                     QTMLSADDRKAMVSKLKTEMQEAVKKLEFERAASLRDQIIRLEK"
     gene            9746..10399
                     /gene="fsa"
                     /locus_tag="PK937_11870"
     CDS             9746..10399
                     /gene="fsa"
                     /locus_tag="PK937_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNH33313.1"
                     /translation="MKFFIDTANLDEIREAASLGILDGVTTNPSLVAREKNVNFHDLL
                     KEICKVVDGPISAEVVSTDLDGMLKEGYELAKIHSNITVKLPLIKSGLQACKRFTSDG
                     IKTNVTLCFSPNQALMAAKAGATFISPFVGRLDDISTNGMQLIEEIVNMYINYGYETQ
                     VLVASVRHPMHVVEAAQIGAHVCTMPYKVIDQLIAHPLTDLGLKKFLDDWAKVKAGK"
     gene            10491..11579
                     /locus_tag="PK937_11875"
     CDS             10491..11579
                     /locus_tag="PK937_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNH33314.1"
                     /translation="MKRWGLIFFFVCFSLVAQDDITSSRQNAITRSVKKALPAVVGIN
                     VVQIQEFTYGNPFFNDDFFSQFFPKQKYYDKVKGLGSGFIISSDGYIVTNHHVINGAV
                     EVVVTLTDGTKYPAKIVGGDKTTDIALLKIEPERKLKYLEFGDSEKLLIGEWVIAMGN
                     PFGLFDINSQPTVTVGVISATNRDFGLVDNDHVYQNMIQTDAAINGGNSGGPLLNSNG
                     EVIGMNTFIYSGSSTSKTSIGIGFAIPIHRIDKIISELKSNGKVDRYFTMGFRYQQVD
                     QMIAKYLGLKELRGIVVSEIIKNGPAENAGLLVGDLIVKINDKPINSEYDFRKITLGD
                     DLRAGDVVDVEVLREKKRLTFQLKLGKV"
     gene            11591..12481
                     /locus_tag="PK937_11880"
     CDS             11591..12481
                     /locus_tag="PK937_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNH33315.1"
                     /translation="MKIGFIVNPRAGTHHNAYEEVSALAGKLVALGHTITLHKTQGPG
                     DATVMARNLVQQGYDVIASAGGDGTANETAQGVMDSDSAFTIIPYGSGNGLARGLAIP
                     LKPSAAVQGIPNGKVRRIDVGQLIDGSQVRLFFGFAGIGYDAYIGRLFNQRKGRRGLL
                     PYVYLSLTAYNGYAPVALTLKADDVLYSGKPFILAIANTDEYGNGARIAPNAVPDDGV
                     FDISMIQSMSFVKGLLNGWRLFNGTIDKVKDVVSLRVQEAEVIPSGKIYYHMDGEARE
                     TNNTLHFKILPGRLKVLVPN"
     gene            12546..13250
                     /locus_tag="PK937_11885"
     CDS             12546..13250
                     /locus_tag="PK937_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH33316.1"
                     /translation="MNDNKSVTRKLETLKIVPIFSGLGIKDLEKIANVAFQRSYTKEQ
                     VVLFEDEGGQTLFIVMTGQVKISRISEDGREVILAVMAEGDFFGELSLLDGQSRSANV
                     VCTKDAEMLLINRDDFLNLLSEFPQIAIQLLRELASRMRKSDSQIKNLSLKNSTGKVA
                     GTIARLAEDIGIPNNGSTIEIPNLPTQQDLANMSGTSRETISRVLQRFINDGYVSKDG
                     NKLVIRDFEKFKKDLV"
     gene            13351..>13920
                     /locus_tag="PK937_11890"
     CDS             13351..>13920
                     /locus_tag="PK937_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33317.1"
                     /translation="MKKKATIVICDPHPDSASALTEILVSNDYALVHAHDGHDVLDKV
                     KSLDYDLALIATDTYAMDGFQVCRLLKESERTRHIPVMLASGMSDKVTRNRAIQAGAD
                     EFITKPYNRVELLSRVVNLLRIKHLHDRLENLISEKETEKRKLEERTRELQLLSDIAR
                     IVISLKEQKIVLAEIIDKISAAFGVEVALL"
BASE COUNT         3680 a         2994 c         3223 g         4023 t
ORIGIN      
        1 cgcgtctccc atcggtaata agtgaaagat gacgtcggga ttgtcccgcg aaacggcgta
       61 ttgtacgtga tgcgtatcac gggatagatt tagaatttct tgtatcgtga ggtgccgaat
      121 cgggcaaagc caatttccgg caatttcgac cgacggtacg agaacaaaat ttcgctctgc
      181 aaaacgtgga tgggggatga ccgtatccgg cgtattcata atgagatcat caaacaaaat
      241 aatatccaaa tcaatcgtgc gtgcgttccc gggaatcgtt ctttcacgac ccaatgattt
      301 ttctacgtct aatagttttt tcatcaggct ctgagcatct aagtccgtat caatgatcat
      361 agccgtattg taataattat cctgcatgat cgggctaacc ggctcggttt cataaatgga
      421 tgccatcttt ttaataccgg caaatcgact gattagatca acagcttcgc gaagatgctg
      481 catccgatcg cctatgttgg agcctaagct aagcaggact ttattcttca tgcagttttt
      541 ctttcacggg tgatttcaac ctccacatga tccaaggaac cgtgaatggg aacactcggt
      601 ttacggacac agaccgtgac gacctgtatt ggaaaatcat taagtagtcg ctgcgcgata
      661 ttttcagcga ccgcctcaat caggtaatat ttcttttccg tgacgacagt gtgaatgcgc
      721 tgatacacgg tgccgtagtc cacgctcaaa tgcagatcat cggcgagcgc cgccggttgg
      781 aaatcgagcc cgagttcaac atcgatttca aagcgctgac ctaagcgccg ctcttcttca
      841 tgtgcaccat gatgagcata aatgatcaga ttgacgagtc ggattttatc gttatttttc
      901 atgttatgtt ttagcaaaag ccgccatacg ccgcacggct tccgagaggc gctcttcgga
      961 ctgcgtcagc gcaatccgaa agtacgatcc gctggactga ccaagcgccg aaccgggtgt
     1021 cacaacgact cctgttttat ccaataactg tttagtaaag atcattgaat ccgaaccgcc
     1081 gggcacttct gtccatacgt aaatggttgc tttgccatga tgacttttga atccggccgc
     1141 attgagccct tgcactaccg tatctttgcg gcgtctatat aattcgatca taggtttcgt
     1201 aaattcttcg caatgatcca atgcaaacgc ggccgcctct tgtaacgcta agaataaacc
     1261 ggtatctatg ttggctttga gtcgggacag tccggcgatc aaggaaggtt cgccgacggc
     1321 aaatccgacg cgccatccgg tcatgttaaa agttttagac agcgaatgga attcaatacc
     1381 accgtcaagc ccgcatgttg tttgcatcag actgggtggc gcttcttcga aaaaaagttc
     1441 ctgatatgcc gcgtcatgcg cagcgatgat gtcgttagtt ttacagaact gagcaatgga
     1501 ttcgaaatca cattgatccg cccaaccggt agtcgggttg ttgggataat tgaaaaacat
     1561 caaccgtgca cgttttacga catcggctgg gatttcattg agcggcatca gaaaacgatt
     1621 ttttgctaat aacggtatcg aatggaacac gccgccggct aatttggttc ctgttacata
     1681 ggccggataa ccgggatccg gtgcaatgac gatttcacca gggtttacaa aagcgatcgg
     1741 aaaatgggca ataccttctt tggagccgat caaaggaacg atattgcgct gagtatctac
     1801 cgtgacatcg tatcgtcttt tcataaaagc cgcaacactt tctctaaaac gcatcgagcc
     1861 ggcttggtag gggtatcggt gattggccgg cttttgtagc gcctgagtgc aagcatctat
     1921 gataggttgt ggggttggaa tatcggggtc acctacaccc agatctataa cgtcttttcc
     1981 ttgggcaatg gattcacgtt tgaggcgatc cacctcggca aaagcatgta caggcaattc
     2041 ttcaaaacga tcagctaaac gaaatgtcat aaaaataata gtaagttcaa aaaaaatctg
     2101 aacaatatac cgtaaaggat attttcattc aagcctgata attcatgtaa aataaaaacc
     2161 ccgcatcgga tgacgaagcg gggttgtacg gtaagtttgt atcaaatcag aaaatgtaat
     2221 tcacgcggaa tgcaaatttg taaggactgc ctttttcgct taccgatttg gcgatatcaa
     2281 atcggaacag gtcattttca tcgccaatac ctacgccgag gtcggtttga atatcgcgca
     2341 gattggttcc acgagccaat tcgcgaatgg cataaatcgt attattccat gcataaccgg
     2401 cgtcggcaaa taaaatggca ctgaacggat gaatggggaa aatgcgatca tttttcagag
     2461 caccggaact gatgcggtat tcgacattag ccaatgccat ttgatttccc gacatcgctt
     2521 tataatcatg accgcgtaag gtggagatgc cgccgaggtg aaacattttc tgagcgggga
     2581 ctgtgccggt caccgatccc aacatcaggc gcatatccag attttcgtaa cggctcagcg
     2641 gttggtagcg tgcgatgctc agaacgtagc gggaaaaatc aaaatcggaa ttccactcat
     2701 cgcggctacg ctcggcttcg ccgtaaatat cccatccaac acgtttgctg tacttgcggt
     2761 tattatagac ccatccgcgc gtattgagtt cagcacgcac aacaacgctg ttcatacgac
     2821 cttcatcaat catagggttt gttcggaagt caattttttt actccgaaaa agcgcccaat
     2881 tcacattatt ggaagcggac gagtagtcat caactttgta ggctgcgctt attttgaatt
     2941 gatggttcag gttttgctga gcttggaaac taaaaccacg tgtacggaaa taatcccgat
     3001 tgtctatttt taacagcatg gaattcatag cattttccat ttgcccaaca atccaattat
     3061 cgttagtcgt ggtcaggtcg tgcacttcac cgcctaaagt aaatcggtat tccgaacccc
     3121 agattttatc cagaccaagg cggtaatgga aatctttagg tccgaaagca taaccggctt
     3181 caccatatat ttgaaacggt ttgttatcat atatcttatg gtcacgatct attttgccgc
     3241 cgatataaag accatctaca cgattgtagt caaaaagaac gaaggatgtg ttgcgaagtt
     3301 tttcctgatg attgtcccaa taattattgc gtgaataata gcgcggactt ctacgatctt
     3361 cattacggcg aggtacatta tccacccgta caaacgaagc gctgtgcgaa cgatcttcgt
     3421 cttcattatc gtcttcatcc gtctggggag tatagttata ttcgtattcc ggcagatctt
     3481 tttcgacggt ttcattcatc cgcgccattt tgcggttaaa tttttccatt ttacgattaa
     3541 atttttccat ctcgcgattg aaccgcttcc aatccgcttc ggtcatgttg tccgcgttag
     3601 gagcttctac cacaggaggc acttcgatgt acggggtaat acttttattc ttacgtttgt
     3661 ttttatagtc atacacccgc gcaccgtcct tacgttggac agtaggtggt gttacggagg
     3721 tttcatcatc ggttaattct tcgggatcat tctcttgata gcttttcgat ttgacggatg
     3781 gcgcataagc cgttacttca cttgatgttt catctgctgt agattcatct gttgtaaaat
     3841 tagattcttg ttttgcggcg atatcttgtt tattcattga gcgctgagag gactttacct
     3901 gcagaatgtt gcctgcaatc tgatcggaat taaccgtttc gacgcggccg ttgatactga
     3961 cggcatcacg agtgaccgaa ccggtttcgc gcatgatgat atttccgttg gaaacgacaa
     4021 ttttaccttc cacagtaccg gcaataataa ggtcgccgtt gagcagtaaa atatcgcttt
     4081 tgatcgtgcg cccttcggga atttcacggt taccgttata tatcaggacc gatttggaat
     4141 cggccaaata tttcgcacgc acatctgcaa caacaccgtc ttcgtcaatc atttcatttt
     4201 gtgccatacc tggaaatgtc cagctaagca tcaataaaca aactgctgtt aaagttttcg
     4261 tgaaaagttt catggtcgcc ccttatctct tatatggtta gctttgtttt ttacggtgcc
     4321 taatcccaaa aagttgcagg atttttatat tttctaaaaa agaccgttta accctaaaaa
     4381 agttgcaatt atatatgctt tatattccct ttttagatgc tttttcgatc tttaaatagt
     4441 tttaatgctg tcgaataatc ttccggacta ttcatgttaa aaaaaacgtc agggtaaatt
     4501 gcagattcaa aatgtgaggc cggcagaacg aggtttttgg tttgattcag gaaatccgtt
     4561 aagcggtaat tttcgtcgcg gatcatcgaa tcaattatag gaagatgttt tttgtggtaa
     4621 aatgcacata gcgtttcaag cccgccgggt gttttggggg cgatcgtatc aaactgaggt
     4681 aatctggatt gcatcagagt ggttacacga tgatcaaaaa ggggtaaatc acaagctaaa
     4741 acaaatacat ggtcatataa tgcggattga agtccggtat agattcctgc catcgctgat
     4801 cgcgacggat gcacatccgt gatgattgag tgatgtagat taagcgctgc taaggagtcg
     4861 gaggatttag ttacgatatg gatttcgcgt gcggttttag ccaattcgtc agctaaattc
     4921 ttaagaaggg gggtaccgta aaagtcgaga agaaacttat cctgacccat tcgttgggat
     4981 agaccgccgg ccagaataat gcccgtaaaa tcatgcatgg ttaaggaata cgtacggctt
     5041 ttttgaattt ttctttagtc gtaagatcaa cgcgatattt ggattgaggc gtatttatta
     5101 tcaagacccc gggttcttcc ttcagatgtg ttccgataaa tcttgctaca tatttagaat
     5161 tgtccaaata tgcgctaacg atacgatttt cgtaatacac ataaaaccag ttgagctgcc
     5221 cgtccttttc ttcttgtgtt tcgcgtatat ccgccgcaac caagtcgaac ggatttttaa
     5281 ataaaatacg ggtttctatg gattccgtcg cattcaaaac tgaaatcgac acgtacactt
     5341 catgcggaac agcagctaga gcggccggac gtgtacgatc cgttttactc ggaggtaact
     5401 gaccggcgat catgctcttg cgttcgttgg gtttattggt gcgttttgcg gctacatcat
     5461 tggatggagt acccggctcg gtagacgttt gagcaggagc ctgtttgtcc gtgtttacaa
     5521 tcggtgcttg ctcgttttga atattttgtt ttttagcaac ttccggtgcc ggttgaagat
     5581 tcattttata aatccagagt cctccggcga tcacagctac ggtcgcagca gcacgccaca
     5641 caaaacgcat cgtcattact tttccgggtg ccggatttgc ggtatgtgat aaatgtagcg
     5701 gagaagatgt atggagcaat tctctaatgc gttgtttcat ggctatgcga ccgtgtgcac
     5761 gcaaagcggc tatggtcatt aattcgcgat caaacatcgc gttaaaatct tcgtttttac
     5821 gtaactcatt cagacggata gattcgttat ccgtcagtct gccgaagaca aacttatcta
     5881 taatttcatt ttcatcaagc cattggcggt cggtcggatt taccatattc tatcgcatca
     5941 tttcgtgtta tttgttttta cttgtgctta gtttttgttt tattgaaatg ctttttcgac
     6001 cattttttcg agttcttttt tgcattgcca ttttttggcc ttggcgacat ctgtattgct
     6061 gaattttagc tcatgggcta tttcttccat gcctttttct tctagataaa acatcatcaa
     6121 cagtttacgg cacgtagcgc ccatgttttc gatacaacgc agaatggtct taaattcttc
     6181 cttttggacc acactgcgca gtgcatcaat ctgttcttcc gccatttcgt aatcggcaat
     6241 atcgcttgtt ttgccgattt tgcgtttatt gatttcccgc agccatttat ttcgtaccac
     6301 agaataaagg tacgtgctga tcttggaagt caagtcgaac tggctttttg ttacattttc
     6361 ccaaagaaca acgatggcat cctgcagcat atcctgcgcg tcgtcttcat ggccactatt
     6421 atccatcaca tatttgcgaa ccatcatgat attattatga tagatcgtga cgagcgcttt
     6481 ttcatcacca cggcgaatac gttcgatcag tatctgatcc gtttctatcg tagcgttttc
     6541 gtaaaccgga gaaccagaat tcatgggccg tttttcttat gttataaata caatttggta
     6601 aagaaggtaa acggaaaata atgaaatatt tatggttttt gatacacttt tcttgcctga
     6661 gccagttttt cgcgactttg ttcgactttt atttcgccga tcagacgtct tatcatcgtt
     6721 tgttcctggg ggtcggctcg cgttttcagg taggcttcaa gttcggttaa tgccgaaggg
     6781 tacattttct gatcgcgata taccaacgcc atacggtatc tcaattccgg ttgtgccaga
     6841 cgttgcaagg cagcgcgctc ataaagaggc agtgcttcag ccaatttgtt ttgtgtaaca
     6901 tacagcattg ctaatgcctg caaataatca gggtcggatg gacgtaatgc taaggctgca
     6961 gtaaaagctg tttctgctcc ggagaaatcg cctttgagca atgcggcgtg acctaaattg
     7021 taatgagccg aaggggattg aggatccaac tggatagctt ttttcaagtg tactaaagcg
     7081 ctgtccaatt tgttgagctt tccgtagcaa ttgccgaggt tgttatgtgc ggctgcaaag
     7141 tccgcgtcaa tgcggattgc ggcgcggtat gatgcggcgg ccttttccgg caatacgagg
     7201 cgttcataaa ctgtgcccat ctgaaaataa gcatcgcggt aatccggaaa aatctgaatc
     7261 gccgtatcgt aaaaggccac ggctgacgaa aactgtccgt tttgctcaag ttctacggct
     7321 ttttcataat aaggttttgc tttttctaga agctgtcgct cattacaacc gccgcaggac
     7381 ttgataacgg tcgcaattaa aagtattgca aaccatgagg tcaatttttt catggtggca
     7441 atataattga acgcggctaa aatgccaacg agaaacagta aaaacaatta tgcggaggat
     7501 ttataatata cggctgtttt ggttttattt ttcgaggcga atgatttgat cgcggaggct
     7561 ggcggcacgt tcaaattcca atttttttac ggcttcctgc atttccgttt tgagtttgct
     7621 taccatggct ttgcggtcat cggcactcaa catggtctga tagtctatcg tcgcttcgga
     7681 ggccgctaaa gcttgagctt cctcctggcc ttctttgtag gtaaaataat cggcaaccga
     7741 ggtggatcgg ataatttgct cctttgattt aaaaaccgtg gtcggggtta taccgtgttt
     7801 ttcgttatgt gccatttgca tggcacggcg acgaccggtt tcatcaatga ctaatttcat
     7861 tgagtcggtc attttatcgg cataaaaaat cacggttccg ttggcattac gcgcagcgcg
     7921 cccggctgtt tgcatcagcg agcgttcgct tctcaaaaaa ccttctttgt cggcatcaag
     7981 tacggcaacg agggaaactt ccggaagatc caagccttct cttagaagat tgatacctac
     8041 caatacgtca aaacccgcca accgcaaatc gcgcaggata tccactcgtt ccaaagaatc
     8101 aatttcggag tgcatatagc gaacccgtat tttgaggttg gctaaatatt ccgtgaggtc
     8161 ttccgccata cgtttcgtta aggtggtgac caacacgcgt tcaccgacct gagttcgttt
     8221 gcggatttcg tggatcagat catcaatctg attttttacg ggacgcactt cgatcatagg
     8281 gtcaagcaaa ccggtcggac gaataatttg ttcaacgatt tctccgccgg atttttgcaa
     8341 ttcatagtcg gcgggggtgg cgctgacata cacgacctga ctcagcattt tttcaaattc
     8401 ttcaaatttg agcgggcgat tatccaaagc ggaaggcaga cgaaatccat attcgactag
     8461 attcgttttt cgtgcgcgat cgccgttata catagcgcgc acctgcgata ccgttacgtg
     8521 ggattcgtct atgaacatca gaaaatcttt ggggaagtag tccagtaaac aggccggtct
     8581 gtcaccttca ccgcggccgt cgatgtggcg cgagtagttt tcgatacccg agcaatagcc
     8641 taactccagc atcatctcga tatcgtattt ggtgcgttgc tccagtcgtt gggcttcgac
     8701 caggcgattt tgactccgga aaaaagtcaa acgctcttcg agttcttttt gtatgcgcag
     8761 aatggcctga tgcaatttgc tttccgaggt gacaaagtgt gtcgccggaa aaataatggc
     8821 cgttgccggt tttgtgataa ttttatgtgt taccggatca agataagcga tttgttcgat
     8881 ctcatcgccg aataactcga tacggacata gtgatcttcg taggccggat agatatcaat
     8941 cacatcaccg cgcacacgaa atgtgccgcg ttcaaaagca atatcattgc ggttatattg
     9001 catatctacc aaacgggcga gcattttatt gcgatcaacg gtttgacctt tttctgtaaa
     9061 aacaaacata tctttgtaat cttgcggtga tccgataccg taaatgcaac ttaccgatgc
     9121 gacaattatg acatcctgac gttccaataa cgaactggta gcgcgaaggc gtaatttttc
     9181 gatttcgtca ttgatggaca tatctttagc aatgtaggtg tccgttgacg cgacgtaggc
     9241 ttcaggttgg taataatcgt aataactgat aaaatattcg acagcattgt ccggaaaaaa
     9301 ttgtttgagt tcaccgtaaa gctgcgccgc cagggtttta ttatgagaga gtacgagggt
     9361 aggtttgccg atctgagcaa tgacattagc catggtaaat gttttacctg aaccggtaac
     9421 acccaataga gtttggtgtt gctgtccgtc tcggatgccc cgcacaagat gggctacggc
     9481 ttctttttga tcgccttgta gggcgtaatc ggatttgagt ttaaattcca tgcatcaaat
     9541 atactcgatc gttgtgacaa ccttatgtca gtatataaaa aataacaatg aaaaaacaaa
     9601 tgtatcaata atgcgcttgt gctttgcata aattttgtat gcagaaatag ccttgattgg
     9661 ataaaatcaa agggctattt tgagaacgtt ctgctaaaat taaccgaaga gttaaggccg
     9721 tttattaacc gcaggaggtc gttgtatgaa gttttttatt gataccgcca atttagatga
     9781 aattcgcgaa gccgcatcgt tgggtatttt ggatggtgtg acaaccaatc catctctggt
     9841 agcgcgcgaa aaaaacgtca attttcatga tttgctgaaa gaaatttgta aagtcgttga
     9901 cggacctata tcggcggaag tggtcagtac tgatctggac ggcatgctca aagaaggtta
     9961 cgaattagcc aaaattcact ccaacatcac ggtcaaatta ccattgatca aatccggatt
    10021 acaggcttgc aaacgtttta cgtcggacgg tataaagacg aatgtgacgc tatgcttttc
    10081 gcccaatcag gctttgatgg ctgcgaaagc gggtgccaca tttatcagcc cttttgtcgg
    10141 tcgtctagac gatatcagca ccaacggcat gcaacttatc gaagaaatcg taaatatgta
    10201 tatcaactat ggttatgaga cgcaagtcct cgtggccagc gtacgtcatc cgatgcacgt
    10261 ggtcgaagcg gctcagatcg gtgcgcatgt ttgtacaatg ccttataaag tgattgatca
    10321 gttgattgcg catccgttga ccgatctcgg tttgaaaaag tttcttgacg attgggccaa
    10381 agtaaaagcc ggaaaataat gattgaatcg gtacgcgccg gaaccttttg gatttaccca
    10441 acgtaagcaa ggttgcatat aatgaagtac aacatttcag gaggttccgg atgaagcgct
    10501 ggggtttaat atttttcttc gtatgtttct ctcttgttgc gcaagatgac attacgtcgt
    10561 cgcgtcaaaa tgcgattacc cgatccgtca aaaaggcgtt acctgccgtc gtcggtatca
    10621 acgtcgtaca gatacaagaa tttacctacg ggaatccttt ttttaatgac gattttttca
    10681 gtcaattttt tccgaagcaa aaatattacg ataaagtcaa aggccttggg tcgggtttca
    10741 tcatctcatc ggatgggtat attgtcacca atcaccatgt gatcaatggt gccgtagagg
    10801 ttgtcgttac attaaccgac ggtacaaaat atcccgctaa aatagtcggc ggtgataaaa
    10861 ctacggacat cgcgttactc aagatcgaac cggagcgcaa actcaaatat ctcgagtttg
    10921 gcgactcgga aaaactgctt atcggcgaat gggttattgc catgggcaat ccgtttggtt
    10981 tgtttgacat caatagccaa cctacggtaa cggtcggggt gatcagcgcg accaatcgtg
    11041 atttcgggct cgtagataac gatcacgtct accaaaacat gatacaaacc gacgccgcga
    11101 ttaacggcgg taatagcggt ggacctttat taaacagcaa tggcgaagtg atcgggatga
    11161 atactttcat ttacagcgga agtagcacat caaaaacgag tatcggtatc gggtttgcaa
    11221 taccgattca tcgcattgat aaaatcatat ccgaactaaa atccaacggt aaagtggatc
    11281 gttattttac gatgggtttt cgctatcagc aggtggatca aatgatcgcc aaataccttg
    11341 gtctaaaaga attacggggt atcgtcgttt ccgaaattat caaaaatggc ccggctgaaa
    11401 atgccggatt gctggtcggc gatctaatcg taaaaatcaa tgataagccg atcaattcag
    11461 aatacgattt tcgaaaaatc acgttaggcg acgacttgcg tgccggcgat gtcgttgatg
    11521 tcgaggtgtt acgcgaaaag aagcgcctta cgtttcagtt aaaattggga aaggtgtaat
    11581 cgaataatag atgaaaatag gttttatcgt caatccccgc gcgggaacgc atcataatgc
    11641 atacgaagag gttagcgctt tggccggaaa actggttgct ctgggccata cgataacatt
    11701 acacaaaacc caaggcccgg gcgatgcgac ggtaatggcg cgaaatttag tgcaacaagg
    11761 atatgatgtt attgcatcgg caggcggaga cggtacggcc aatgaaacgg cgcagggcgt
    11821 tatggattcc gattcggctt ttacgattat cccttacggt tcaggcaacg gtttggcacg
    11881 cggattggcg ataccactta agccatccgc ggcagtacaa ggtattccga acggaaaagt
    11941 tcgccgcatt gatgtcgggc aactgattga tggctcacag gtgcgattat ttttcggatt
    12001 tgccggtatc ggttacgatg cctacatcgg gcggcttttt aatcaacgca aagggcgccg
    12061 cgggcttctg ccgtatgtat atttatcgct tacggcgtac aatggttatg cgccggtggc
    12121 gcttacattg aaagcggatg atgtgctgta ctccggcaaa ccctttatct tagcgatagc
    12181 taatacggat gaatacggta atggtgcgcg tatcgcacct aatgccgtac cggatgatgg
    12241 tgtctttgat atttccatga tacagtccat gtcatttgtt aaaggtcttt taaacggatg
    12301 gcgtttattc aacggtacga ttgataaagt caaagatgtg gtcagcttgc gtgtgcagga
    12361 agccgaggtg ataccgtccg gtaaaatata ttatcatatg gatggcgaag cgcgcgaaac
    12421 aaataacacc ttacatttta aaattctgcc cgggcgcctc aaagtgcttg tacctaatta
    12481 gttcttgatt gcagcatgcg aatatcgtac tttgtgccgt tccaaaatcc cttcaaatag
    12541 tacctatgaa cgacaacaaa tccgtaacgc ggaaacttga gacgctaaaa atagtaccta
    12601 ttttttccgg ccttggtatt aaagacctgg agaaaatagc gaacgtagct tttcaacgca
    12661 gttacacaaa agagcaggtt gtgcttttcg aagatgaggg cggccagacg ctttttattg
    12721 tgatgaccgg gcaagtcaaa atttcgcgca tcagcgaaga tggccgcgaa gtaatattgg
    12781 ctgttatggc ggagggggat ttttttggcg aattgtcgtt gctagatggc cagtcgcgtt
    12841 cggctaatgt ggtttgcact aaagatgcag agatgcttct tattaaccgg gatgattttc
    12901 tcaacttact tagtgaattt ccgcaaattg ctatccaact tctgcgtgaa ttggcaagtc
    12961 gtatgcggaa atccgattct cagattaaaa atctatcgct taaaaattcg accggcaaag
    13021 tagccggcac aattgcacgt ttagccgaag atattggtat ccccaacaac ggcagtacca
    13081 tcgaaattcc caatttaccg acgcagcagg acttggccaa tatgtcagga acaagtcgcg
    13141 agacgatctc ccgtgtacta cagcgtttta tcaacgatgg ttatgtatct aaagatggta
    13201 ataaacttgt gattagagat tttgaaaaat ttaaaaaaga tttggtttaa gaataaatat
    13261 ggaatgcagc ccaaagccag ccctacggtt ggcttttgtt tttttggctt ttgaaaaggc
    13321 gcttccgtat attgcccgcg cataaatcat atgaagaaaa aagccactat cgtaatttgt
    13381 gacccgcatc cggactccgc gtccgcattg acggaaattc tcgtctccaa cgattatgct
    13441 ttggttcatg cacatgatgg gcacgatgtg ctcgataagg taaaaagtct cgattatgat
    13501 ctggcgctga ttgctaccga tacgtatgcg atggacggtt ttcaggtgtg ccgtttgctt
    13561 aaagagagcg aacgaacgcg gcatattccc gtgatgctgg cttccggtat gtctgataaa
    13621 gttacgcgta atcgtgcgat acaagccggt gcggacgaat ttatcacgaa accgtacaat
    13681 cgtgtcgaac tgttgtcgcg tgtcgtcaat ctcttgcgta tcaaacatct gcacgatcgt
    13741 ttggaaaatt tgatatccga aaaagaaacc gaaaaacgta aattagaaga acgcacccgc
    13801 gagctgcaac ttctatcgga catagcgcgg attgttattt cgctcaaaga acagaaaatc
    13861 gtattagcgg aaattattga taaaatatcg gccgcgtttg gcgtcgaagt tgctctgctc
//
LOCUS       DAOQGF010000092        13910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2043_length_13910_cov_6.263732, whole genome shotgun sequence.
ACCESSION   DAOQGF010000092 DAOQGF010000000
VERSION     DAOQGF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2043_length_13910_cov_6.263732"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1327)
                     /locus_tag="PK937_11895"
     CDS             complement(155..1327)
                     /locus_tag="PK937_11895"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNH33318.1"
                     /translation="MINQTDVIIVGAGAAGLMCAMTAARRGRRVVVLEQNEQVGKKIL
                     ISGGGRCNFTNLLSGPDNFISENPHFFKSALSRYSPTDFVELVKKHNIAFHEKKNGQL
                     FCDHSAKDIVSMLLSECADGGVRMVMKNRVEEVRYDECYTLQTNTGIYKAPHVVIATG
                     GLSIPKIGATDFGLRLAEKFGHRIVPPEPALVPLQMDAGFIQRYGDLTGISIEVIISC
                     EKKYFHENMLFTHQGISGPAVLQISSYWKKGDILTIDLLPNKTIEQLLDQYMDSRSDL
                     VNVLSYHFPKRFIQSWCVEHFSNKPVYQLSSKELNNLDEKIHRWKLIPVATAGFEKAE
                     VTRGGVDTRDISSKTMESVKQNGLYFVGEVVDVTGHLGGHNFQWAWASGHAAGLFV"
     gene            complement(1487..1915)
                     /locus_tag="PK937_11900"
     CDS             complement(1487..1915)
                     /locus_tag="PK937_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33319.1"
                     /translation="MRQKWSLIVIVIVLLQCFLLKSCISNDQQDDLRVGLSVLTDKSL
                     YHNERIYIHISNKTESIFYLLLYNSAFREKIVKDQWSLVTSSLIDDPQSDTVIINMNE
                     ATVMDIFVVKPGIYRVGIPFLWNGDPGRKDTVYSNEFEVY"
     gene            complement(2020..3552)
                     /locus_tag="PK937_11905"
     CDS             complement(2020..3552)
                     /locus_tag="PK937_11905"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNH33320.1"
                     /translation="MKMFSNEPVKLKKADRPLILAIDIGTSSTRAVVYDAQGRSVKKL
                     VHQVGYMMQVTPDGGVFADPKFIVDATAQCIDAIMEAMGKDANDIKAVGFDTFWHNMM
                     GIDMRGKPTTPLINWADTRPRIVIDDLRKRIDPEEAHRLTGCVLHPSYLPAKLLWMHK
                     YNKEDFRRTTTWMSIGEYLYYVFFGKVICGISMASGTGLFNQNTCNWDEKILSVLPIK
                     PGQLGELGDLDTPLRGLKSEYARRWPALAQIPWLPAVGDGASSNVGTGAVKKDQLCIV
                     MGTSGALRVMWEAKDVTIPSELWVYRVDRKRFLMGGALSDGGNLRKWMLSRLAIEGGK
                     KSVDAALNSTKPDAHGLTMLPFWAGERSTGWHQNARGMINGFNLSTTREDILRVTLES
                     LCYRYAAIYEIFKKNQMPVKQILASGGGFVDSGALTQLAADVIGFPITQSGEAEGSAR
                     GAAILALESIGAIKNIDMAPVPLGKTYKPHPKDNAIYKNARQRQQRLYDLVAKGIWEE
                     NL"
     gene            complement(3681..4232)
                     /locus_tag="PK937_11910"
     CDS             complement(3681..4232)
                     /locus_tag="PK937_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33321.1"
                     /translation="MKTIRMISVFLLAGIGGCGDDTTALNDPWEAFAEGRYQDAHSLF
                     LDLIETKPSEAYAGLGWTTLKMNADSLNAANRYFSLSSNDSLVYAFAGWALASWASEN
                     YMDCIKYGDFVLRKNPEFVFDYLQTLTQKQIALAQAYSYFHLQNLSACISKIRIIEPS
                     YTPVNNLDRNEIQLKLDQLRQTQ"
     gene            complement(4256..9673)
                     /locus_tag="PK937_11915"
     CDS             complement(4256..9673)
                     /locus_tag="PK937_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33322.1"
                     /translation="MKQRITIIFGVLYFFHQISVSAQVGTWENVGGDISGPSANAILK
                     MGSDVYVGGSFWGAAGVSGADNIVRWDGLQWVALGEGLNGTVNDMVFVGNDLYVGGFF
                     EDAGGDPNADFLARWNGVSWSAVGTGVSGYVDALATDGTNLYVGGNFTNAGGIANTFC
                     IAKWNGSAWSALGSSGLNSTVRTIAMINSTTFYAGGVFSNIGGDPNKSRIVKWNGSDW
                     EAVGAGLNSDVTSIAISGADVYVGGLFTNVGDVVGANYIAHWNGSTWSALGSPSEAVS
                     SVAVVGTEIFVAGNSNISNDAANPSGVAKWNGSSWESFGNFNSTVSALLASGGDLYIG
                     SPLTGSSSPIVWNGSTFSFFGSGLAQSVNTIAVSGENIFIGGGFTNAFQNSNINYIAM
                     RNGAVWSSLGSGLNGSVKSIAVSGSDVYVGGQFTDAGGITTADHIARWNGSAWSAMGN
                     INPGVQILSMAITGSTVYAGGVFTDLSGVSGASGIAKWNGATWEALGDGLFGGEGEAY
                     ITSIVISGSDVFVGGYFQNAGGISDADNIARWDGSTWHAVGNGLNEGVSCMVMNDGLL
                     YVGGEFSDVVGGVSNNLNRLAVWNGSAWQPLGSGLGNEDYVYSLACIGSEVYAGGNFE
                     EIGSTAATNIAKWNGIAWESVASGLNNTVNQMAVSSNDLIVGGEFTKSGTEVANKLAV
                     YHAPTPANPLVVTNNSDDNVVGTLRYATNYANAHPGLDTIRFDASMIGQKILTEGITI
                     PDETLVNGDINGDRAPDIAIQLINTVSDYALQIGNQVTIHAINISGSKNGIRITGSQN
                     TISNSYFGTNLSGSDTTGGFANQFTAIYGESAASFITIEGNLLAGRKDINGVTGLLLN
                     GGRGHLIRGNVFGTDKNGNLDLGFKNSSVVIDAKAFSFGDGSFSGRNIVVNNGNGGTT
                     VYLGGDSVRVLGNFVGLWKDGVTAKSNGSGMSVVGNYAQIGDGSNSGRNVIGNCANAA
                     LVIQGNYGRVLGNYIGTDSTGIVARGNTQGVYVGSGSHSVYIGDGTADGRNIICSNNI
                     GIYSDPAGTDSVYVLGNWIGIGADGVTLLTNKLYGILFNAHSHHNMIGDGTIGGRNVI
                     SGNNDGSARAMLVSSRHNRILGNYIGVLPDGMTLAFNNDGIWLENDSNHIGDGTAGGR
                     NIISGNSNGIVITSKARNNKILGNYIGIASDGAASVPNVRGIFSLSSTHLQIGDGTSG
                     GRNIISGNTQVGIRLTSTDSALVFGNYIGVGSDALTPMANSIGLQLDNASFSNRIADN
                     IIANSSQYGIQTSDATTLKNTFFKNRIYKTTIQPISQVSGAQESVWPPAIFANGGNVY
                     TGLASKGALVQFFADSSDEGLMFLDTVFADPSTGKWSKTLIVPSGMNLTAMQDSAGNS
                     SVFSTAYEPIRNVAPSIPVLLTPQSNALTNNLRPTFTWHKSIDGDDDDVFYQILVSKN
                     SDLSDAQLTVSNITDTTFTSGTDLDSTFGYYWRIIAVDNKGGAAQSAVRFFKTDAKKP
                     KLTASLHVMKAIERYVQVYIASDETLVSRTVSFSFSGNTIQKTPDLIADGLYQVNYEI
                     ATSEGLLSVIIQGGDSAGNVQSSSLSYQVTSLNKKSGVSISHQMLTLDVPPNATSGDG
                     LLMVTSFKSDQNIHKVNSVDLVPVSDTYELISAVSIMRPWNLTIRFNELESSEWDERK
                     VSLYAQRDGQWVYVGGEGRHGSVTAKISGASRYRLFYNPSHVILPVKIQLDQNFPNPF
                     NPTTSIRFGLPQDGHIRVTVYNILGQFIAELLNENRTAGYHNVIWNGRNATNQQVSSG
                     IYLYRLETSQGVITRKMMLLK"
     gene            complement(9818..10459)
                     /locus_tag="PK937_11920"
     CDS             complement(9818..10459)
                     /locus_tag="PK937_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH33323.1"
                     /translation="MIKVAVVEDDKIIRQSLQIIIDGTDGFRCIGAYPNAESFIDDVQ
                     RLKPEVVLMDIHLKNSMSGIEATRILRKAYSRLVILIQTVYEDDNTIFDALCAGANGY
                     LLKKTSPARLIDSIREAVEGGAPMSPAIAKKVVAHFQQNNIVSQNSEDARLSDRELDV
                     LRLIADGRPYKQASELLSVSVPTIRFHMGNIYKKLHATSQSEAIAKAIRRGLI"
     gene            complement(10530..13421)
                     /locus_tag="PK937_11925"
     CDS             complement(10530..13421)
                     /locus_tag="PK937_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH33324.1"
                     /translation="MPSKYCAWSILLILCFIKLCPYPAAILLAQTHIHRNISTDHGLP
                     QSQVTALMRDTKGYLWIGMLGGLARWDGNEFYSIRLEDGSSPGGIRSLHEHVNGRIFA
                     ATNGQGLLEIIGQRAILHDKRSGLPDMSVRTIASDTDGALWVGTDKGLVRFTDAILDS
                     ASARIFFTGIVISDVKAYKNKVYAGCFDNGLYEFLNDTFEVLFPSLSSDIPKIRSFIY
                     VEAENEWYVAVDGRGTAKITNNSLTWLAPLSDYRVQTMRWIKDGGVFFTTIGNGVYNL
                     RNGKWLHIGEDQGLANNSVWAIETLENGWIALGTWGGLDFYDGGRIRSYRRENGLQDP
                     VVLAIEEDPRQAIYFGTATGGVSMLYKNKWQHWNKKNGFPDLTIWSLLSTSRENFFVG
                     TNNFGIIDITPSGSKKLYGKAEGLSENRVYVMRERSDGSVFIGHREGLDVLDKGKIYS
                     YPKGHRWNRAFITGIHAVSDDEIYLATRYGVMHVNKGIVDTINRKKGLSDDHVWNVYV
                     DSAQYIWASTNNGGITIFSNSTIDTINTRNGMSDNTVYGVIQYKENIYYAATHKGINR
                     IEIKNGHFEINVITKSEGLPSDELNQGAFRKNSDGTMWFGTTKGAVHYDPRMEVRQPT
                     PPNVHITRVRAFDNELLAKSASDVISLPYNENYIKFDFVGLNLHAPERVVYQYRLSSI
                     DREWVTSEHHFCQYPNLSDGTYTFEVRASNEYGLWSEICSYRIIIEPPYWKTWWFIML
                     VTLAGGGLISLIVYSRIQAMLAVERLRNKIAADFHDAIGAGLTQVHYLTQTALMHSEK
                     TEQVRHNLTIIDAISKSLYEEMRDIIWLVNPRRDSLYELILRLKESSEDMAEQSGVEF
                     ITENIDSLSGLRLAMERRSHIYLIFKEATANALKYSRCKTLTLTAHVKGDRLSIVLSD
                     DGVGFEDAHPRAGGNGMHNMKTRALEANGQIEIHSEPNKGTRVTFTGKI"
     gene            complement(13456..>13910)
                     /locus_tag="PK937_11930"
     CDS             complement(13456..>13910)
                     /locus_tag="PK937_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33325.1"
                     /translation="EDNLDIICDLFIKQYDFVTIEKKYTGVPAVYCNTAEINQALVNI
                     LTNAVQAIQDRGLKEGQIDLSTSTQTHEQNEYVVISIRDNGIGMSSSVMDKIYDPFFT
                     TRDVGSGRGLGLTEAYGIIQRHHGKIEVWSEVGKGSEFTVYLPVRKRP"
BASE COUNT         3998 a         3395 c         2656 g         3861 t
ORIGIN      
        1 acggcaatgg atatacctac ggcataggaa atatatttac gataaacgag ttttagttgg
       61 ctttgaatca tatattaata aaacgaattc tggcacgaaa tgttccctaa actaatatcc
      121 gtgttttttt ttattagcgc aagattgaaa aatattaaac aaaaaggcct gcggcatgcc
      181 ccgacgccca tgcccactga aaattatgcc ctccaaggtg acccgttaca tctacaacct
      241 caccgacgaa atacaaacca ttctgtttaa ccgattccat tgttttcgac gaaatatcgc
      301 gtgtatcaac acctccgcgt gtaacttcag ctttttcaaa tcctgctgtt gcgaccggta
      361 tcaatttcca ccgatgtatt ttctcgtcca aattatttag ttctttagaa gaaagttgat
      421 aaacaggttt attcgaaaaa tgctccacgc accatgattg tataaatcgc ttaggaaaat
      481 gataactcaa tacgttaacc agatctgaac ggctatccat atattgatct aacaattgtt
      541 ctatcgtttt attcggcaaa agatctatgg ttagaatatc tccttttttc cagtaagaag
      601 aaatctgcaa aaccgccggc ccactaatac cctggtgtgt gaaaagcata ttttcatgaa
      661 aatatttttt ttcgcatgaa ataataactt cgattgaaat acccgtcaaa tcaccgtacc
      721 gctgaataaa accggcatcc atctgaagag gaactaacgc cggttcaggc gggactatcc
      781 gatgaccaaa cttttcagcc agtcgcaatc caaaatccgt agcgcctatt tttggaattg
      841 aaagccctcc agtcgctatg acgacatggg gtgctttgta aataccggtg ttagtttgta
      901 acgtatagca ttcatcataa cgaacttctt caacacgatt tttcataacc atcctgactc
      961 cgccatcggc acattcgctc aacagcatgg atacaatgtc cttcgcggaa tgatcacaga
     1021 ataactgacc gttctttttt tcatgaaaag caatattgtg tttttttacc aattcaacaa
     1081 aatctgtggg actataccgc gataaagctg atttaaaaaa atgagggttt tctgaaataa
     1141 aattatcagg tcccgataaa agattggtga aattgcaacg cccaccgcct gaaatcagaa
     1201 ttttttttcc tacctgttca ttttgttcta aaactacgac gcggcgtcct cttcgagccg
     1261 ccgtcatagc gcacatcaga ccggccgcgc cagcgccgac gatgatgaca tccgtttgat
     1321 ttatcatagt gaattaacgt gcaattattt tactcttttg agatcgaatt ggtattatga
     1381 ctgatgagta aaatactgaa ataaacggat aatgatgaaa gaaaaaaatt aaatgcgttt
     1441 ataacaattt tcttaaaaaa agaattcaaa attcaataac tggacttcaa tatacttcaa
     1501 actcattaga atacacggta tccttacgtc caggatcacc attccataaa aaaggaatcc
     1561 cgacacgata aatacctggt tttacgacga aaatatccat taccgttgcc tcattcatat
     1621 taataataac tgtatcgctt tgaggatcat ctattagcga tgaagtaacc aaactccatt
     1681 gatctttaac aattttttca cgaaaggcgg aattatacag taacaaataa aaaattgatt
     1741 ccgttttatt ggaaatatgt atataaatac gctcattatg atatagtgat ttatcagtta
     1801 aaactgataa gccaactcta agatcatctt gttgatcatt tgaaatacat gactttagaa
     1861 gaaaacactg taatagaaca atcactatca caattaaaga ccatttttgc ctcataatcc
     1921 atcctccctc gataatttct attatcttag tactggtgag acacaaaaac ttacagatta
     1981 tttaaaaaat aaaaccctcg ttaccgaggg ttttaataat tacaaatttt cttcccaaat
     2041 acctttggca actaaatcat aaagtctttg ctgccgctgc ctagcatttt tatagatcgc
     2101 attatccttt ggatgcggct tatatgtttt tccaagaggt acgggagcca tatcaatatt
     2161 tttgatagcc cctattgatt ctaaagccaa aattgccgct ccacgcgcag agccttcggc
     2221 ctcaccggat tgggtgatcg gaaatccgat cacatccgct gccaattgtg tcaatgctcc
     2281 tgaatcaaca aaacctccgc ccgaagccaa tatttgcttc acgggcattt gattcttttt
     2341 aaagatttca taaatcgcgg cataacgata acataagctt tccaatgtca cgcgtaagat
     2401 atcttcacgc gttgtgctga gattaaagcc attaatcatg ccgcgcgcat tctgatgcca
     2461 accggtactg cgttcaccag cccagaaggg taacatcgta agtccgtgtg catcaggttt
     2521 agtggagttg agggctgcat ccacactttt ttttccgcct tcgattgcta aacggcttaa
     2581 catccatttg cgtaaattac cgccatcact caaagcacct cccataagaa atcttttgcg
     2641 atcaacccgg tacacccaca attcggatgg gatagttaca tccttcgcct cccacataac
     2701 ccgtagcgcg ccggaggtac ccattacaat acaaagttga tcttttttta cagcaccggt
     2761 ccctacgttg gagctggcgc catcaccaac agccggaagc catggaattt gagccaaagc
     2821 cggccaacga cgcgcatact ctgatttgag cccgcgcaaa ggcgtatcca aatcgccaag
     2881 ttcaccgagt tgacccggct tgatcggtag tacgctcaat attttctcgt cccaattgca
     2941 ggtgttttgg ttaaaaagcc ccgtacctga tgccatagaa atgccgcaaa tcactttccc
     3001 aaaaaaaacg tagtacagat attcgccgat ggacatccat gtggtcgttc ggcggaaatc
     3061 ctctttatta tatttatgca tccacaataa cttcgccggt aaataactgg ggtgaagcac
     3121 gcatccggta aggcgatgtg cttcttcagg atcaatgcgt ttacgaaggt cgtcaataac
     3181 tatgcgtggt ctggtatccg cccaattgat gagcggtgtc gtcggtttac cacgcatatc
     3241 aatacccatc atattatgcc aaaatgtatc aaaccctact gcttttatat cattggcatc
     3301 ttttcccatc gcctccataa tcgcatcaat acattgcgcc gtagcatcca cgataaactt
     3361 aggatcggca aaaacaccgc catctggcgt tacctgcatc atatacccta cttgatgaac
     3421 taattttttg actgaacgtc cttgtgcatc atacaccact gcacgcgttg aggaagtccc
     3481 gatatcaatc gctaaaatta acgggcgatc agcttttttg agtttaacgg gttcgttaga
     3541 aaacattttc atacgcgtct ccggtgatgg tacaattagg atacataagg caggcgcctc
     3601 attttagcca tttccatgaa tgccttcaag cagaaaaaaa tcccgcatcg ttagtacggg
     3661 atttttatgt cttaatttat ttattgagtt tgtcgcaact ggtcaagttt gagctgaatt
     3721 tcattgcgat ctagattatt gaccggcgtg tacgaaggtt ctatgatacg tatcttagaa
     3781 atgcacgccg ataaattttg aagatggaaa tagctatacg cttgcgccaa agcaatctgc
     3841 ttttgagtca gcgtttgtaa atagtcaaaa acaaattcag ggttttttcg caatacaaag
     3901 tccccatact taatgcagtc catatagttt tctgaagccc atgacgcgag ggcccaaccg
     3961 gcgaatgcat aaacaagtga atcatttgat gatagactga aatacctgtt tgccgcgttc
     4021 aatgaatcag cgttcatttt gagagttgtc caacccaatc cggcataggc ttccgacggt
     4081 ttcgtttcta ttaaatccaa gaaaagagaa tgcgcatcct gataacgtcc ttctgcaaaa
     4141 gcctcccacg gatcgttgag agccgtcgta tcgtccccgc acccacctat tcctgccaaa
     4201 agaaatactg aaatcattct tatagttttc atactatttt ccttcgttat tgatgttact
     4261 tcaaaagcat catttttctc gtaatcacgc cttgcgaagt ttctaagcga tataaatata
     4321 tcccgctaga aacttgctga ttggttgcat tacggccatt ccaaatgacg ttatgatagc
     4381 cagctgttcg attttcattg agcaactccg ctatgaattg gccgagtatg ttgtacaccg
     4441 tgacacgaat gtgcccatcc tgcggtaacc caaatcgaat agatgtcgtc ggattaaaag
     4501 gatttgggaa attctgatct aactgaattt ttacaggtaa gatcacatgt gaaggattat
     4561 aaaaaagccg atatcgggaa gcgccagaga tctttgctgt aaccgagcca tgacgtcctt
     4621 cacctccgac ataaacccac tgtccgtccc tctgggcgta gagagaaacc ttgcgttcat
     4681 cccattcgct actttccagt tcattaaaac gaatagtcaa attccatgga cgcattatag
     4741 atacagcgga gattaattcg tacgtatcac taaccggaac taagtccaca gaattgactt
     4801 tgtgaatatt ttgatcggat ttaaatgacg ttaccataag aagtccgtca cccgaggttg
     4861 cattgggtgg aacatccaga gtgagcattt gatgtgagat acttacgccg gattttttat
     4921 tcaatgatgt aacttgataa ctaagcgacg acgattgcac attaccagct gaatccccac
     4981 cttgtataat aacagataac aaaccttccg atgttgcaat ttcgtaattg acctgataca
     5041 atccgtcggc tatgaggtcc ggtgtttttt gtattgtatt tccactaaag gaaaaactta
     5101 ctgttcggga taccagcgtt tcatcactag caatataaac ttgcacatag cgttcgatcg
     5161 ctttcatgac gtgaagactg gccgttagtt tgggtttttt ggcatcggtt ttaaaaaaac
     5221 ggactgcact ttgcgcagcg cctcccttat tatcaaccgc tattatacgc caataataac
     5281 cgaatgtaga gtccagatcc gttcccgaag taaatgtcgt gtccgtgata tttgaaacag
     5341 ttaactgcgc atctgataaa tcggaattct ttgataccaa aatttgataa aacacatcgt
     5401 catcgtcccc atcaattgac ttatgccacg taaatgttgg acgaaggtta ttagtcaatg
     5461 cattcgattg cggtgttaat aacacaggaa tagagggcgc aacattgcga atcggttcgt
     5521 aggccgtcga aaagaccgaa ctattgccgg cgctgtcttg catggctgta agattcatac
     5581 cggatggaac gattagtgtc ttggaccact tacccgtgga aggatcagca aataccgtat
     5641 cgagaaacat cagcccctcg tcggatgaat cagcgaagaa ttgcacaagg gcgcccttag
     5701 atgccaatcc cgtataaaca ttacctccat tggcaaatat agccggaggc cacacgcttt
     5761 cttgagcgcc gctgacttgg cttatcggct gaattgttgt tttataaatt ctatttttaa
     5821 aaaacgtgtt ttttaatgtc gttgcatccg acgtttgaat accatactgc gagctatttg
     5881 caatgatatt atcggcgata cgatttgaaa atgaagcgtt gtcaagttgc aggccgatac
     5941 tgttagccat cggtgtgaga gcatctgagc cgacaccgat atagtttcca aatacaaggg
     6001 cgctatccgt cgaagtcaga cgaatgccga cttgcgtgtt gccggagatt atgttgcgcc
     6061 cgcccgatgt gccatcgccg atttgcagat gtgttgatga taaggaaaaa atgccccgca
     6121 cattgggcac cgatgccgcg ccatcagacg caataccgat ataatttcca aggattttat
     6181 tgtttcgggc tttacttgtg atcacgatgc cgtttgaatt accggaaatg atattgcgtc
     6241 ctcccgcagt accgtcaccg atatggttag aatcgttttc cagccaaatg ccgtcgttat
     6301 tgaaggcaag cgtcatccca tccggcaaaa caccgatata gtttcctaaa atccgattat
     6361 ggcgcgagct gaccaacatg gctcgtgcag acccatcatt attaccggag attacattgc
     6421 gtcctccgat cgtaccgtcg ccgatcatgt tatgatggga atgtgcattg aacaaaatgc
     6481 catatagttt attggtaagc aatgtcaccc catccgcacc gatacctatc caattgccca
     6541 gtacatatac cgaatctgtt ccggccggat cggaatagat tccgatatta ttggagcaaa
     6601 taatattacg tccgtccgca gtaccgtcac cgatgtatac cgagtggctt ccgctaccga
     6661 cataaacacc ttgcgtattc cctctggcga cgattccggt agaatcagtt cctatataat
     6721 ttccaagaac tcgtccgtaa tttccctgaa taactaacgc cgcattagca caattaccga
     6781 taacattacg tcccgaatta ctaccgtcgc caatctgagc ataattacct actactgaca
     6841 tccccgatcc attggattta gccgtaacgc cgtccttcca cagacctacg aaattaccca
     6901 atacacgaac cgaatcccct cctagatata ccgtagtgcc gccattacca ttgttgacta
     6961 caatatttcg accgctaaaa ctgccatcac caaacgaaaa tgccttagcg tctataacga
     7021 cagacgaatt tttaaaacct aaatctaggt taccattttt atcagttcca aaaacgtttc
     7081 ctctaatcaa atggccgcga ccaccattca gtaaaagccc cgttactcca ttgatatctt
     7141 ttcgtccagc caaaagattg ccttcaatcg taataaagga agccgcgctt tcaccgtaaa
     7201 tagcagtaaa ctgattggca aatccgcccg tcgtatcact accggacaaa ttggtcccaa
     7261 aataagaatt ggaaatcgta ttttgtgagc ctgtgatacg aattccattt ttgcttcccg
     7321 agatattaat tgcatgaatt gtaacctgat ttccgatttg cagagcatag tctgaaactg
     7381 tattgataag ttggattgcg atatcgggtg cacgatcgcc attgatatca ccgttaacca
     7441 atgtttcatc cggtattgtg atgccttcag tcaaaatttt ttgcccgatc atagatgcat
     7501 caaaacgaat ggtgtccaaa ccgggatggg cattggcata gttggtcgcg tagcgcaatg
     7561 taccaacaac attgtcatca ctgttattgg ttaccaccaa aggattggcc ggtgtaggcg
     7621 catgataaac agctaattta ttggccactt ccgtacccga cttcgtaaac tcgccgccta
     7681 cgatgagatc atttgatgat accgccatct gattaacagt gttgttcaat ccacttgcta
     7741 ccgattccca tgcaataccg ttccatttgg cgatattcgt tgcggccgtg ctacctattt
     7801 cttcaaagtt gccgccggcg taaacttcgc ttccaataca tgctaaggag taaacataat
     7861 cctcgtttcc aagcccggat ccaagtggtt gccatgcgct tccattccat acggctaagc
     7921 gattcagatt attcgaaacg ccaccaacca catcgctaaa ttctccgcca acgtacaata
     7981 aaccgtcatt cataaccata catgaaacac cctcattgag cccattgccc acagcatgcc
     8041 atgtactacc gtcccatcgt gcaatattgt ctgcgtcgga aataccaccg gcattctgaa
     8101 aatatccgcc aacgaaaaca tctgagcccg aaatcacgat gcttgtaatg taggcttctc
     8161 cttcgccgcc aaataaacca tcccccaacg cttcccaagt agcgccgttc catttagcaa
     8221 ttccgctagc accggacaca ccactcaaat ccgtaaagac gccgccggca taaacagtcg
     8281 aaccagtgat agccattgat aaaatttgta caccgggatt aatgttgccc atcgccgacc
     8341 acgctgaacc attccatcta gcgatatgat cagccgtagt tataccacca gcatcagtaa
     8401 attgtccacc gacataaaca tcgctgcccg ataccgcgat ggacttcacc gacccgttga
     8461 gaccacttcc caacgatgac cacacagccc catttctcat cgcgatatag tttatatttg
     8521 aattctgaaa agcattggtg aatcctccgc cgataaaaat attctctcct gacacagcga
     8581 tcgtattgac cgattgagca agtcctgatc caaaaaaact gaacgtgcta ccattccaca
     8641 cgatcgggga gcttgacccg gtgagtggcg atccgatgta gaggtcgccg ccggaggcta
     8701 agagtgctga taccgtgctg ttaaaattgc cgaaactttc ccagcttgat ccattccatt
     8761 ttgctacgcc tgatgggttt gctgcatcgt ttgaaatatt agaattgcct gctacaaaaa
     8821 tctccgtacc gactacggcc acactcgata ccgcttccga aggagaacct aaagcggacc
     8881 aagtagaacc attccagtgt gcaatatagt tagccccaac aacatcaccg acattcgtaa
     8941 ataaacctcc cacatataca tctgccccgg agattgcaat ggaggtgaca tccgaattta
     9001 atccggcgcc caccgcttcc caatccgatc cattccattt cactattcgt gacttattgg
     9061 gatcgccacc gatattgcta aacacgccgc ctgcataaaa agtagtactg ttgatcatgg
     9121 cgatagttct gactgtgctg ttgaggcctg acgaacccag cgccgaccac gcgctgccat
     9181 tccatttagc tatacaaaat gtatttgcaa tgccgccggc atttgtaaaa tttcctccga
     9241 cataaagatt agttccatcc gtagccaacg catctacata accgctaacg ccggttccga
     9301 ctgcagacca agacacgcca ttccatcgcg ctaaaaaatc cgcattggga tcacctccgg
     9361 cgtcttcaaa aaaaccgccg acatacaaat cgtttccaac aaaaaccatg tcgttgaccg
     9421 ttccgttcag accttcgcct aaggccaccc attgcaaacc gtcccatcgg acaatattgt
     9481 ctgcaccgct aacacccgcg gctccccaaa aacttccgcc aacatacaca tcggaaccca
     9541 ttttgagtat cgcattcgct gaagggcctg aaatatcgcc gccaacattt tcccacgtac
     9601 cgacctgtgc cgagacactg atctgatgaa agaaatatag aacgccgaat ataatcgtaa
     9661 tccgttgctt cataaacgat cctcctgatg ggtattaccg ttggttatga atcaaaataa
     9721 aacagactgt ttatacggaa tactgtcaga atggataaat agcgaagtat gaatgagaag
     9781 caacctatct tatctgatag gtcgtcaaaa ggtaggatca gatcaaaccg cggcgaatgg
     9841 ctttggcgat ggcttcactt tgcgaagtgg cgtggagttt tttataaata ttacccatat
     9901 gaaaacgaat cgtaggtacg ctgacggaaa gtaactcgga agcttgttta taaggtctgc
     9961 cgtcagcgat aaggcgaagc acatccagct cacgatcaga gaggcgagcg tcttcagaat
    10021 tttgcgatac aatattattt tgctgaaaat gagcaactac ttttttggca atcgccggac
    10081 tcatgggcgc cccgccttcg acggcttcgc gaatactatc aattaatcgt gccggagaag
    10141 tttttttcag cagataacca ttagctccgg cgcataacgc atcaaaaatc gtattgtcgt
    10201 cttcatacac ggtctgtata agaatcacca aacgactata tgctttacgt aagatacgcg
    10261 tcgcttcaat accactcata ctgtttttaa gatgaatgtc catcaacacc acctccggtt
    10321 ttaaccgttg cacatcatct atgaaagact cggcgttggg gtaagcccct atacaacgga
    10381 acccgtcggt gccatcaata attatttgaa ggctttggcg gataatttta tcgtcttcga
    10441 caacggccac tttgatcata catatctcat atgatttttt ttaacgtatg ttatattgga
    10501 ttcgattaca actggcacaa gtgatagttt tatattttgc ctgtaaacgt aacacgcgtt
    10561 cccttgtttg gctcagagtg aatctctatt tgcccgttcg cttctaaagc tcttgttttc
    10621 atgttatgca tcccattgcc gcctgcacgg ggatgggcgt cttcaaaacc aacgccgtca
    10681 tcgcttaata cgatggaaag ccgatcacct tttacgtgtg cggtaagagt caatgtctta
    10741 cagcgactgt attttaaggc attggcggtg gcctctttaa aaatcaaata aatatgacta
    10801 cgccgctcca tcgccaaacg taagccggat aacgaatcaa tgttttctgt aataaactcc
    10861 actccggact gctccgccat atcttccgag gattctttga gccgaagaat caattcgtat
    10921 aacgaatccc gacgcggatt gacaagccat atgatatcgc gcatttcttc atacagcgat
    10981 ttggatatag cgtcaattat agtaagatta tgccgcacct gctccgtctt ctccgaatgc
    11041 atcaacgctg tctgtgtcag ataatgcact tgggtcagac ccgctcctat cgcatcatga
    11101 aaatcggctg caattttatt tctcaaacgt tcaacagcga gcatagcctg aattcggcta
    11161 taaacgatca acgaaatcaa tccacctccc gctagcgtta caagcataat gaaccaccac
    11221 gttttccaat agggaggctc tataatgata cgatatgaac aaatttcgct ccaaaggccg
    11281 tattcattag atgcacgcac ctcaaatgta taagtaccat cgcttaaatt cggatattga
    11341 caaaaatgat gttcggatgt tacccattcg cgatcaatgg atgaaagccg atattgatac
    11401 acgacacgct ccggtgcgtg tagattgaga ccgacaaaat caaattttat ataattctca
    11461 ttatatggaa gggaaatgac gtcggaagcc gacttcgcaa gtaattcatt atcgaacgca
    11521 cgcacacgtg ttatatgtac attcgggggc gtcggctggc gcacctccat tcgtgggtcg
    11581 taatgcacgg cacctttggt cgtaccaaac cacatcgtac catcgctgtt cttccgaaag
    11641 gcaccctgat ttaactcatc actgggcaat ccttcggatt tggtgataac gttaatttca
    11701 aaatgcccat tttttatttc gattcggtta atgcccttgt gtgtagcggc ataatagata
    11761 ttctccttgt actgtataac gccgtaaact gtattatccg acatgccgtt gcgtgtatta
    11821 atcgtatcaa ttgttgagtt agaaaaaatg gtaataccac cgttgtttgt cgaagcccat
    11881 atatactgcg cggagtccac ataaacgttc catacatgat catcagaaag gccttttttc
    11941 cgatttatcg tatctacgat acctttattc acatgcatga caccatagcg cgtcgcaagg
    12001 tagatttcat catctgatac ggcatgaatg cccgtaataa aagcacggtt ccagcgatgt
    12061 cctttcggat aggaataaat ttttccttta tccaaaacat ccaatccttc gcgatggcct
    12121 ataaatacgc taccatccga acgctctcgc atcacatata cccgattttc agacagtcct
    12181 tctgcttttc catacaactt tttcgaaccg gagggtgtaa tatctatgat accgaaatta
    12241 ttagttccga caaaaaagtt ctctctcgat gttgaaagta agctccatat ggtgagatca
    12301 ggaaaaccat tttttttatt ccaatgttgc catttatttt tatacagcat ggatacgccg
    12361 cccgtggctg taccgaaata aatagcttga cgcggatctt cttcgatagc caaaactacg
    12421 ggatcctgaa gaccattttc acgtcggtac gatcggatac gtccaccgtc ataaaagtcc
    12481 aaaccgcccc aggtacccag tgcgatccat ccattttcta aagtttcgat agcccaaacc
    12541 gaattattag ccagtccttg atcttctccg atgtgcaacc atttaccatt acgcaaatta
    12601 taaacaccgt ttccgatagt agtaaaaaac acacccccgt cttttatcca gcgcattgtt
    12661 tgcaccctat aatcagaaag cggtgcaagc cacgtcaaac tgttatttgt tatttttgcc
    12721 gtgccgcggc catccacagc gacataccat tcattttcag cttcaacgta gataaatgat
    12781 cgaatcttag gtatgtccga ggacagcgaa gggaataaaa cttcaaacgt atcattaagg
    12841 aactcataca atccgttgtc aaaacatccg gcataaactt tattcttgta cgctttaacg
    12901 tccgaaatca caatccctgt aaaaaaaata cgcgctgaag ccgaatccag tatcgcatcg
    12961 gtaaaacgga ctaatccttt gtctgtgcct acccacaatg caccgtctgt atccgacgcg
    13021 atcgtgcgca ccgacatatc aggcaggccg cttcgtttat catgtaagat cgcccgctgt
    13081 cctatgatct caagcaaccc ttggccattc gtagctgcaa aaatacgacc attcacatgc
    13141 tcatgcaggg atcgaatccc acctggagat gaaccatctt caagtcgaat cgaataaaat
    13201 tcattaccat cccaacgcgc caatccgccc agcatcccaa tccataaata tcctttggta
    13261 tctcgcataa gcgctgtgac ttgagattga ggtaagccgt gatcggtaga aatatttctg
    13321 tgtatgtgcg tctgagccaa taagatcgca gcggggtacg gacatagttt tataaagcac
    13381 aatatcaata gtatgctcca cgcgcaatac tttgaaggca tcataaataa gacacgatat
    13441 ttgaaagttg tagaattatg gccgcttgcg aaccggtaag tacactgtga actccgaacc
    13501 ttttcctact tcactccaca cttcgatctt gccgtgatgc cgctgaataa tcccgtatgc
    13561 ttccgttaga ccaagcccac gtcccgaccc cacatcacgt gttgtaaaaa acggatcgta
    13621 aattttatcc ataacggacg aagacatgcc aatgccattg tcgcgaatcg aaatgacaac
    13681 atattcgttt tgttcatggg tctgtgtgga tgtggataaa tcaatttgac cctcttttaa
    13741 tcctctatcc tgtatagctt gcaccgcatt cgtgagaata ttgacaaggg cttgatttat
    13801 ttcggccgta ttacaatata cggcaggaac tccggtatat tttttttcaa tcgtaacaaa
    13861 atcatactgc ttgataaaaa gatcacaaat aatatcaaga ttgtcttcta
//
LOCUS       DAOQGF010000093        13813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2059_length_13813_cov_6.398823, whole genome shotgun sequence.
ACCESSION   DAOQGF010000093 DAOQGF010000000
VERSION     DAOQGF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2059_length_13813_cov_6.398823"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1458)
                     /locus_tag="PK937_11935"
     CDS             complement(82..1458)
                     /locus_tag="PK937_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNH33326.1"
                     /translation="MSLLLIGILLYVALQLLIGVFVSRKIHSESDYLLAGRSLGMGLA
                     TFSIFATWFGAETCIGTAGAAYESGWAGVKADPFGYGVCVLIVGIFFAIRLWKQKLTT
                     IGDFFRARYSRSVEKTAVILMIPTSIMWAAAQIRAFGLVLSASSELTITLSVTIAAVV
                     VVTYTAFGGLLADAMTDVVQGITLIIGLLVIMPFLISDTGGIEGFMGIILQERHNETN
                     VNFLEIAETWAIPICGSLVAQELLSRVIASRSPQIAQRSSLLAAILYVSVGIIPVLIG
                     LTGFVVLPGLSESESILPMMAQKYLTPMLYILFSGALVSAILSTVDSTLLAASSLMTH
                     NLIIASRRDLDEKLKLRISRWGVIFMGILSYILALYAEGIYDLVKDASALGSAGIFIV
                     FVFGLFTNWGGAYSALASLIVGTAVWIGAHYAWESSTGYLTALGAAFIVYVGIGFFEK
                     HTKPITPN"
     gene            complement(1461..2093)
                     /locus_tag="PK937_11940"
     CDS             complement(1461..2093)
                     /locus_tag="PK937_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33327.1"
                     /translation="MRFIKKNYWIWALALIAQVSSFVVAQSKPEPCMINESFSLAKHK
                     PEKIAQCIEEQYNKSLNSPGTVLYDSKDLNMFFVKGVRLPDVNKNLSSFGKRLYADCF
                     EYRGGPILAFSDDKPDSTDETVRRYVFGSAVVLTDKKGIFEIHCFDSKYEAAKGVKVG
                     MAITEVVAKFPGLKLTNEKEGDVLISNKGCLTVHYTDSIVRWMVVARGCY"
     gene            complement(2102..3007)
                     /locus_tag="PK937_11945"
     CDS             complement(2102..3007)
                     /locus_tag="PK937_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNH33328.1"
                     /translation="MKRMLLIATVILMACDGGKSDEALILDLTDFPLPLAASTAAIYN
                     GELYVFGGSDNYNGDTTYQSIFKFNGSSWETVDTMFNDATWDGKVLIKENFAYYVSGW
                     PDISGALKRYNFTTKTWTGLDTGGIGSNWGTTAQIVGNKIYHFDSYGEVEVYDMNTNQ
                     WLSRNPNGLKNGSERGLTSVVHDGNIFVTGYGDTVLYKYHVDADSFSVESVMPRSITS
                     CVMVELENKIYFIGGSENDEEAYVYSDSYIYDITEQEWTHSSIALKTPSIYTAYVLSG
                     STVYVIGGINASGDGITDAYKISLK"
     gene            complement(3127..5739)
                     /locus_tag="PK937_11950"
     CDS             complement(3127..5739)
                     /locus_tag="PK937_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNH33329.1"
                     /translation="MTMEEQRIIDARSHEKPWKKWGPYLAERQWGTVREDYSPYGTAW
                     EYLTHDQARSKTYRWGEDGIAGISDEKQILCFAIALWNKKDPILKERFFGVTGTEGNH
                     GEDVKEYYYYLDSTPTHSYMKMLYKYPLEAFPYGQLVEENRRRTKLEPEFELADTKIF
                     DNDNYMDVFVEYAKNTPEDILIRVTIHNRSGQKATVNLLPQIWFRNTWAWGYDSYKPR
                     LHAENHAIHVTHAELGAMTLFSKEKGELLFCENETNYSKLYNMPPGSGYYKDAFHEYL
                     IGNNRKAVNPDMEGTKAGINYDISVEAQNSVTVLLRLGPSRMNDPFSDFDTVFQNRIK
                     ECDEFYATLQKKLNNDDMCLIQRQALAGMLWSKQFYYFDVAQWLAGDPGQPSPPSERK
                     KGRNHAWTHLNNADILSMPDKWEYPWYAAWDLAFHCIPLAMVDIDFAKHQLTLLTHEW
                     YMHPNGQLPAYEWQFGDVNPPVHAWAAWRVYSVEKMKCGAGDLHFLKSIFHKLLLNFT
                     WWVNRKDSEGNNVFEGGFLGLDNIGVFDRSAELPTGGKLEQADGTSWMAMYAVNMMRI
                     ALELARFDKVYEDMATKFFEHFLLIAGAIADTGKGGGDLWDEEDQFFYDVLRAPGIGE
                     MRLKVRSMVGLTPIFAVEILDTQSLHEVPTFAKRLEWFLQHRPDLAKLVSRWQEPGIG
                     EKRLLSLLRGHRLKQLLKRMLDENEFLSDYGIRALSKFHQKNPYVFQSNGKSFTVNYQ
                     PGESESEVFGGNSNWRGPIWMPMNYMLIASLYKFHSYYGDDFLVEHPTGSGNYMTLTA
                     IADDLSKRLTRIFLRNDQGQRPIFGDIAKFQKDPFFKDYILFYEYFHGDNGCGVGASH
                     QTGWTGLIAKLLEA"
     gene            complement(5878..10131)
                     /gene="rpoC"
                     /locus_tag="PK937_11955"
     CDS             complement(5878..10131)
                     /gene="rpoC"
                     /locus_tag="PK937_11955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNH33330.1"
                     /translation="MSIYLSNNSLSKDFSTITIGISSPDAILSRSYGEVTKAETINYR
                     SFKPEKDGLFCEKIFGPVKDWECHCGKYKRIRYKGIICDRCGVEVTLKAVRRERMGHI
                     SLAVPVVHIWFFKSLPSKIGSLLGLSIKQLEKIIYYENYIVVQPGKSGLKYKDIIPEE
                     ELYRVKEELGLDNENLPNTDPNKFIALIGGEAVKELLKRVDVEQICIDLRKVLRDPST
                     SIQKRSDALRQLRVADAFRQSEGAIENRPEWMVLDVVPVIPPELRPLVPLEGGRFATS
                     DLNDLYRRVIIRNNRLRKLIEIRAPEVILRNEKRMLQESVDSLFDNSRKTAAVRSDSN
                     RPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKEMAVELFKP
                     FIIRKLVERGYVKTVKSAKRMVDKHGAEVWEILEKIVSDHPVMLNRAPTLHRLGIQAF
                     QPVLVEGRAIQVHPLVCAAFNADFDGDQMAVHIPLSYEAQIEARMLMLSSHNIIQPSS
                     GRPIAIPSQDIVLGNYYLTKAKSGARGEGMRFSSIAEVRQAFDNGVAELHAKIKLRYV
                     KGEGKTATVAYIDTTVGRSLFNEIIPKEMGFVNELMTKKKIEETVSRMHRAAGNLKTV
                     EFLDNLKMLGFMTATRAGVSIGIEDVLTPPNKEGVLKQASSQVEKVQKTYRMGLMTDG
                     ERYNKIIDIWTHASNDVAASMYESTQTDRGGFNPIFMMMDSGARGSRDQIKQLGGMRG
                     LMSKPQKSMTGQVGEIIENPITASFKEGLSVWEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVIITEDDCGTILGVKTMAIKEGEEIKESLGERCLGRVAADDVIDPSTG
                     KVAIEAGELIDEAKAEMLNNSAIEMVEIRSVLTCESKRGVCAKCYGRNLATGNVVNMG
                     EAVGVMAAQSIGEPGTQLTLRTFHIGGVAARVGELPQALSKSDGKVVFAPGMKMLERQ
                     TDGIVISLSRNGRIELLDEDRLVYAYKVPYGAELSVHDGQKVKKDDLIMTWDPYNTPI
                     ISEHDGIVHYEGLKEGVTCREEADEATGMKHLVVIESRDKKMTPSVVIKKGREVLETH
                     ILPTRAYLVIKDGEEVHAGEAVAKIPREMAKTKDITGGLPRVQELFEARRPKDSSAVA
                     EIDGTVKIGKISRGYREIMIEGKDGESKSYKIPFGKHLIVHEGDYVIAGEQMSDGSVA
                     PHDILRIKGPAEVQRYLVNEIQEVYRTQGVKINDKHIEVIVRQMLQRVRIQDPGDTYF
                     LQDDQVDRLRFNEENQGIRDKVVVSDKGDSNLKEGQLISRIEIEKICKKLEKKRPPQY
                     REAQPATAEPLLLGITQASLSTESFISAASFQETTKVLADAAINGKTDYLLGLKENVI
                     MGRLIPAGTGLKRYQDIAVTSKDVEEEAASGKKTKSAKK"
     gene            complement(10196..>13813)
                     /gene="rpoB"
                     /locus_tag="PK937_11960"
     CDS             complement(10196..>13813)
                     /gene="rpoB"
                     /locus_tag="PK937_11960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNH33331.1"
                     /translation="TGLQNVFLSNFPVDDAKELFKLEFLKYYVERPKYDVRECQERGV
                     SFSVPLKATLRLISKKSADDKHPEIIEQDVYLGNLPYITEKGTFVINGAERVIVSQLH
                     RSPGVFFDESLHPNGTKMFSARVIPFRGSWIEFTTDINDVMYVFIDRKKKFPVTTFLR
                     ALGYSSDRDMLKLFNIIDEVEISKIDGDKIMGRKLALDIVNEETGEIIAERETELTEE
                     TFAKLKRSKIKKIDLIKAEEVIPEVIANTIRKDVTKDEKEALETIYRQLRSGDAPDLE
                     TARGLLERQFFNSKRYDLGDVGRNRINKRLSLNIPESVTVLTKDDIIAIIKALIELRD
                     GIRQTDDIDHLGNRRVKTVGEQLAGQFSVALTRMTRTIKERMNLRDNENLTPQDLVNA
                     RTVSSVINTFFGTSQLSQFMDQTNPLSELTHKRRLSALGPGGLTRERAGFEVRDVHYT
                     HYGRLCPVETPEGPNIGLISSLCLFARVNRYGFIETPYRKVVKGRVQENIEFLIASDE
                     DGMTIAQANEPIDKKGSFDNEFVKARKEGDFPVVTPDEIQFMDISPAQIVSPAAALIP
                     FLEHDDANRALMGSNMQRQAVPLLVPEAPIVGTGLERTVAVDSRSVIISDIDGVIEDV
                     SATEITIRKKTMGGKKGKDSVSDTMTYRLLKFFRTNQDTCINQRPIVSVGDEVKAGQI
                     IADGCSTERGDLALGRNVLAAFMPWRGYNFEDAIILSERVIRDDVFTSIHIEEYEIQV
                     RDTKRGEEELTREIPNVSEEATKDLDENGVIRVGAEVLAGDILVGKVTPKGETDPTPE
                     EKLLKAIFGDKAGDVKDASLKAPPGMKGIVISTKLFSRKKKDTESKKEDKKKLEELEN
                     ELKKQVRRLKSNLRDELTKLLVGKVSAGVVYTDGSEFLKKGKKFTEDSFKNLDPAEIN
                     LQESRWTDDEETNEMANFLFDNFERNAEEVETEYNSEKFKVQVGDELPPGIVQLAKVQ
                     VAKKRKISVGDKMAGRHGNKGVVSKIVPTEDMPFLSDGTPVDVILNPLGVPSRMNLGQ
                     IFETSLGWAGKILGKHYETNVFDGAKISDVKNELRLAGLPDHGKVRLTDGRTGDRFDQ
                     ETMVGYIYMMKLNHLVDDKIHSRSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAY
                     GAAYILQEILTYKSDDVGGRAKVYEAIVKGDNLPEPGVPESFNVLVRELQGLGLEVKI
                     E"
BASE COUNT         3710 a         3387 c         2862 g         3854 t
ORIGIN      
        1 atgttgaatt cttgatgata atcgcactta tgtttgcacg agctactcag taaggtgtgt
       61 gaaaagagcg attttcgtta attaatttgg tgttatgggc ttagtgtgct tttcaaaaaa
      121 accaattccc acatacacta taaaagccgc accaagcgca gtaaggtagc ccgtcgaaga
      181 ttcccacgcg tagtgagcgc cgatccatac ggctgtacct actatcaaac tcgctaaagc
      241 gctatacgca ccaccccaat tcgtaaacaa cccaaaaaca aaaacgatga aaattcccgc
      301 actaccgagg gccgacgcat ctttgaccaa atcataaata ccttcggcat ataatgctaa
      361 gatataggat aaaataccca taaagataac gccccatcgg gatatacgaa gttttagctt
      421 ttcatctaaa tcgcgacgcg acgcaatgat caagttatgt gtcatcagtg atgaagctgc
      481 caaaagcgta ctgtccaccg tagataaaat cgcagacacg agcgctccgg aaaaaagaat
      541 atataacatc ggagtaaggt atttttgggc catcatcggc aaaatacttt cactttcgga
      601 tagtcccggc aaaacaacaa aaccggtcaa cccgatcaac acagggataa tacccacact
      661 gacatacagt atcgccgcaa ggagcgatga acgttgtgcg atttgaggag agcggctcgc
      721 aatgacgcgc gaaagcaatt cctgcgcaac gagcgaacca catatcggta tagcccatgt
      781 ctcagctatt tctaaaaaat tgacatttgt ttcattatgc cgttcctgca aaataatacc
      841 cataaaacct tctatcccac cggtgtcgga tatcagaaat ggcatgatca cgagtaatcc
      901 gataatgagc gtgataccct gcactacatc cgtcatagcg tcggctaaaa gacctccgaa
      961 tgccgtgtac gttacaacga ccacagcggc gattgtcaca gaaagtgtaa tggtaagttc
     1021 tgacgaagcg gataacacta agccaaacgc acgtatctgt gcagcagccc acatgatcga
     1081 tgtggggatc atcaggatga cagctgtttt ttcaacagaa cgtgagtatc tggctctaaa
     1141 gaaatcaccg attgtcgtta gtttttgttt ccacagacga attgcaaaaa aaataccgac
     1201 gatcaaaaca catacgccat aaccaaaagg atccgctttg acgccggccc aaccgctttc
     1261 ataggccgct ccggccgtgc cgatgcatgt ttcagcacca aaccaagtcg caaaaatcga
     1321 aaatgtagca agtcccatac ccaggctccg ccctgcaagg aggtagtcgg attccgaatg
     1381 gattttgcgt gaaacgaata cgccgatcaa aagttggagc gctacataca acaaaattcc
     1441 aataagcaac agtgacatga ctaataacaa cccctcgcta caaccatcca acgtactata
     1501 ctatcggtat aatgcacagt caggcaacct ttgttagaaa tcaacacatc accttctttt
     1561 tcattggtaa gtttcaaacc gggaaattta gcaacaacct cggtgatcgc cattccgact
     1621 ttcactcctt ttgcagcttc atacttacta tcaaaacagt gtatttcaaa tatacccttt
     1681 ttatctgtaa gtactacggc tgaaccgaat acataacggc gtaccgtttc gtccgtacta
     1741 tccggcttgt catcagaaaa cgccaaaata gggccaccac gatactcaaa acagtccgca
     1801 tataatctct taccaaaaga agatagattt ttatttacgt caggcaatct tacgcctttt
     1861 acaaaaaaca tgtttaaatc tttactatcg tagagtactg tgcccggact gttaagtgat
     1921 ttattatatt gttcctcgat acattgcgcg attttttcag gtttatgttt agccagacta
     1981 aacgattcat tgatcataca aggttcgggt tttgactgcg cgactacaaa tgatgacact
     2041 tgagcaatca aagctaaagc ccaaatccaa taattttttt taataaaccg cataggacct
     2101 cctattttag agatatttta tatgcatcag taataccatc accactggca ttgataccgc
     2161 caatcacata caccgtactc ccggataaca cataagcggt ataaatggaa ggagttttta
     2221 atgcaataga gctgtgagtc cattcctgtt ctgttatatc gtaaatatat gaatctgaat
     2281 acacataggc ttcttcatca ttttcgctac cgcctataaa atagattttg ttttctaact
     2341 caaccatcac gcaagatgta attgaacgcg gcattacgga ttctactgaa aacgaatcgg
     2401 catcaacatg atatttatac aacaccgtat ccccgtaacc tgttacaaag atatttccgt
     2461 catgtaccac cgaagtcaaa cctctctccg atccgttttt taagccgttc ggattacgtg
     2521 ataaccattg attggtattc atatcataaa cttcgacttc accgtaagaa tcaaagtgat
     2581 aaatcttatt gcccacgatc tgagctgttg ttccccaatt cgaaccaata ccgccggtgt
     2641 caaggccggt ccatgttttt gtcgtaaaat tataacgttt tagcgcgcct gaaatatccg
     2701 gccatcctga aacgtaataa gcaaaatttt cttttatcaa aacctttccg tcccaagtag
     2761 cgtcattaaa catcgtatct accgtttccc aactacttcc attaaattta aaaatggatt
     2821 gatacgtcgt atcgccattg tagttatcac tgcctccgaa tacgtacaat tcaccgttgt
     2881 atattgccgc tgtcgaagcc gctaaaggca gcggaaaatc ggtcaaatct aggatcaagg
     2941 cctcatctga ttttccacca tcgcaagcca taagaataac tgtggcaatc aatagcattc
     3001 gtttcataga tttctccggg ataagtatat aaaaatttat ttaagttaat gcatattcag
     3061 catacaggac ctgtcaaaaa cggatattaa gctatttcgt acattaccaa caaatacagc
     3121 ctaaaattaa gcctccaaaa gttttgcaat caaccctgtc catcctgttt ggtgggaggc
     3181 gccaacacca cacccattat cgccgtgaaa gtattcataa aataaaatat agtctttgaa
     3241 aaaaggatct ttctgaaatt tcgctatatc tccaaaaatg ggacgctggc cttgatcatt
     3301 tcttagaaaa atccgagtta accgttttga caaatcatcc gctatagccg tcagcgtcat
     3361 atagttacct gaacctgttg gatgttctac caagaaatca tcgccataat aactatgaaa
     3421 cttatataac gaagctataa gcatatagtt catcggcatc catatcgggc cgcgccaatt
     3481 actatttcca ccaaaaactt ccgattccga ctctccgggt tgataattaa ccgtaaacga
     3541 tttgccatta gattgaaata cataaggatt tttctgatga aatttagaca atgcacggat
     3601 cccatagtca gataaaaatt cgttttcatc taacatccgc ttaagaagtt gtttgaggcg
     3661 atgtcctcgc aaaagagata ataaacgttt ttctccaata cctggctctt gccaacgcga
     3721 aacaagtttg gccaaatcag gtctgtgctg gagaaaccat tccaatcgtt ttgcaaatgt
     3781 aggcacttcg tgtaaagatt gtgtatctag aatttcgact gcaaatatgg gtgtaagacc
     3841 caccatcgag cgcactttga ggcgcatttc accaataccc ggtgctctta gaacatcata
     3901 aaaaaattga tcttcttcat cccagaggtc accgccccct ttacctgtat ctgctatagc
     3961 gccggctata agcagaaaat gctcaaaaaa cttggttgcc atgtcttcgt aaactttatc
     4021 aaaacgcgcc aattccaaag ctatacgcat catattgacg gcatacatcg ccatccagct
     4081 cgtaccgtcc gcttgttcta gctttcctcc cgtaggtaat tcggcgctac ggtcaaaaac
     4141 accgatatta tccaaaccga ggaagccgcc ttcaaaaacg ttattgcctt cggaatcttt
     4201 gcgattgacc caccatgtga aattgagaag taatttgtga aaaatggatt ttaaaaaatg
     4261 aagatccccc gccccgcatt tcattttttc aacactatat acacgccagg cggcccaggc
     4321 atgtacaggg gggttgacgt caccaaattg ccattcgtac gccggcagtt gtccattggg
     4381 atgcatgtac cattcatgag taagcagcgt caattgatgc ttggcgaaat caatatccac
     4441 cattgctaac ggaatacagt gaaacgccaa atcccatgcc gcataccagg gatactccca
     4501 tttgtcgggc atagatagga tatccgcatt attcaggtgg gtccaagcat gattacggcc
     4561 tttttttctt tcgctaggcg gtgaaggttg tcccggatct ccggctaacc actgtgctac
     4621 gtcaaaataa taaaactgtt tactccacaa catgccggcc aaagcttgtc tctgaattaa
     4681 acacatgtca tcattattca attttttttg aagcgtagca tagaattcat cgcattcctt
     4741 tattcgattt tgaaaaacag tatcaaaatc agaaaacgga tcgttcattc gtgacgggcc
     4801 taaccgtaga agaacggtaa ccgaattttg ggcctccacc gaaatatcat aattgattcc
     4861 cgctttcgtt ccctccatat ccgggttgac ggcctttcgg ttattaccaa ttaagtattc
     4921 gtgaaatgcg tctttgtaat atcccgaacc cggcggcata ttatataact tagaataatt
     4981 ggtttcgttt tcacaaaaaa gaagctcccc cttttccttc gaaaataacg tcattgctcc
     5041 aagttcagcg tgggtcacat gtatggcatg attttcagca tgaagccgcg gcttataact
     5101 gtcgtacccc catgcccaag tattacgaaa ccaaatctgg ggtaataaat tgaccgttgc
     5161 tttttgcccg ctgcgattgt gaatggtgac acgaatgaga atatcttccg gtgtattttt
     5221 ggcgtattca acgaatacat ccatataatt atcgttatca aatattttag tatcggcaag
     5281 ttcaaattcc ggttcgagtt tagtgcgtct tcgattctct tccacaagtt ggccatacgg
     5341 gaaagcttca agtggatact tatacaacat tttcatatac gaatgtgtcg gcgtactgtc
     5401 gagataataa tagtactctt ttacatcttc gccgtggttt ccttcggtgc ccgttacgcc
     5461 aaaaaatcgt tctttaagta taggatcttt cttattccac agcgctatgg caaaacataa
     5521 aatttgtttc tcatccgaga tgccggcaat accgtcttct ccccaacgat atgttttaga
     5581 acgcgcctga tcatgagtga gatattccca tgccgtacca tagggactgt aatcttcgcg
     5641 aacggtaccc cattggcgtt ccgcgagata aggcccccat tttttccagg gtttttcatg
     5701 tgatcttgcg tcaatgatac gctgctcttc cattgtcata tgagggactc tttcgtacaa
     5761 agattggttc tatagacggc gtgttatgcc aatgtaatac atagtttccg acagaacaaa
     5821 aaaaaagtga tgctgtcatt taacagcatc actttatatg atcgtatttt tatacgacta
     5881 tttttttgcg gacttagttt tctttccgct tgccgcttct tcttcgacat cctttgaagt
     5941 aacagcaatg tcttggtaac gcttaagtcc ggttccggca gggattaatc ggcccatgat
     6001 cacgttttct ttaagtccga gcagataatc tgttttaccg ttaatcgctg catccgcaag
     6061 cactttggta gtttcctgga aggaagctgc cgaaataaag ctttcggtgc taagcgaagc
     6121 ttgggtgatt ccaagcaata acggttcggc cgtcgccggc tgcgcttcgc gatactgagg
     6181 cgggcgtttt ttctccagtt ttttgcagat cttttcgatc tcgattcggc tgataagttg
     6241 tccctctttg aggttggaat cacctttatc agaaaccacg actttatcgc ggatgccttg
     6301 attctcctca ttaaaacgaa gacggtcaac ctgatcatcc tggaggaaat acgtatcgcc
     6361 cggatcctga atacgaacgc gttgcaacat ctgacgtacg atcacctcga tgtgtttatc
     6421 gttgatcttc acaccttgag tacggtacac ttcctgaatt tcgttgacca agtagcgttg
     6481 tacttcggca gggcctttga tacgtaaaat atcatgcggt gcgacagacc catcggacat
     6541 ttgttcgccg gcgatcacgt aatcgccttc gtgtacgata aggtgcttac cgaaaggaat
     6601 tttgtaagac ttgctttcac cgtcttttcc ttcgatcatg atttcgcggt aaccgcggga
     6661 gattttacca attttgaccg taccgtcaat ttcagccacg gcagacgaat ctttgggacg
     6721 gcgtgcctca aagagttcct gtacgcgcgg gagaccgccg gtaatgtctt ttgttttcgc
     6781 catttcacgc gggattttgg ctaccgcttc accggcatgc acttcttccc catctttgat
     6841 aacgaggtaa gcgcgagtcg gtaaaatgtg ggtctccaga acttcgcgac cttttttgat
     6901 cacgacgctc ggggtcatct ttttatcgcg ggattcgata actacaaggt gcttcatacc
     6961 ggtagcttca tcggcttctt cacggcatgt cacgccttct ttcaatcctt cataatgaac
     7021 aataccatca tgttcggaaa taatcggcgt attgtacgga tcccatgtca tgataagatc
     7081 gtcttttttg actttttggc cgtcatgtac gctcaattcc gcaccgtagg gaactttgta
     7141 tgcgtatacc aaacgatctt catcaagcag ttcgatacgt ccgttgcggc ttaacgaaat
     7201 cacgatacca tcggtttgac gttcgagcat tttcatacct ggagcgaaaa caactttacc
     7261 atccgattta gaaagcgctt gcggtaattc accgacgcgt gcagccacac cgccgatgtg
     7321 gaatgtacgc agagtaagct gtgtacccgg ttcaccgatg gactgggcag ccataacacc
     7381 gacggcttca cccatattta caacgttacc ggttgcaagg ttacgtccgt aacacttagc
     7441 acatactccg cgtttggatt cgcaagtaag tacggaacga atttcaacca tttcgatggc
     7501 tgaattattc agcatctcag ctttggcttc atcaataagt tcaccggctt cgatagccac
     7561 tttaccggtc gaaggatcta tcacgtcatc cgcagcaaca cggccgaggc agcgttcacc
     7621 gagtgactct ttgatttctt caccttcttt gatagccatc gttttaacgc caaggatcgt
     7681 gccacaatcg tcttcagtta tgatcacgtc ttgagcaacg tccacaagac ggcgggtcaa
     7741 ataaccggcg tcggctgtct tcaaagccgt gtcggcaaga cctttacgcg caccgtgcgt
     7801 tgagataaag tactcccata ccgacaaacc ttctttgaag ctggccgtga tcgggttttc
     7861 gatgatttca ccgacctgac ccgtcatact cttttgcggt ttggacataa gtccgcgcat
     7921 accaccgagc tgcttgatct gatcacgcga accgcgagcg ccggaatcca tcatcatgaa
     7981 aataggatta aaaccgccgc gatcggtttg tgtactttca tacatcgaag ccgccacatc
     8041 attggatgcg tgggtccaga tgtcgataat tttattgtaa cgctcaccgt cggtcatgag
     8101 acccatacgg tatgtttttt gaactttttc gacttgcgaa gaggcttgtt tgagtacgcc
     8161 ttctttattc ggaggcgtta atacgtcctc aataccgata gacacaccgg cacgcgtcgc
     8221 cgtcataaaa ccaagcatct tgaggttatc aagaaattca acggtcttaa gatttcctgc
     8281 agcgcgatgc atacgggaaa cagtttcttc gattttcttt ttcgtcatca attcattgac
     8341 aaatcccatt tctttgggga tgatttcatt gaaaagagag cgacccaccg tcgtatcaat
     8401 atacgccacc gtagccgttt tgccttcacc tttgacataa cgcaatttga ttttggcatg
     8461 caattcggca acgccgttat caaatgcctg acgaacttcg gcaatagatg agaaacgcat
     8521 cccttcaccg cgtgcaccgg acttcgcttt agtcagataa tagttaccca aaacaatatc
     8581 ctgcgaagga attgctatcg ggcgaccgct ggaaggctga ataatattat ggctcgaaag
     8641 catcagcata cgcgcttcga tttgcgcttc gtaagacagc gggatgtgaa cggccatctg
     8701 atcgccgtca aagtctgcgt taaatgcggc gcaaaccagc gggtgcactt ggatcgcacg
     8761 gccttcgacg agcaccggtt gaaacgcttg gataccaagg cggtgcaacg taggagcgcg
     8821 gttaagcatt acagggtgat cggaaacgat cttttccaat atctcccata cttccgcacc
     8881 atgcttatcc accatgcgtt tagctgactt gacggttttt acataaccgc gttcgaccag
     8941 tttacgaata atgaacggct tgaataattc gacggccatc tctttgggca aaccacactc
     9001 atgaagtttg agttcaggtc cgacgacgat cacggaacga ccggaataat caacgcgttt
     9061 tccgagcaag ttttgacgga aacgaccttg ttttcctttg agcatgtccg acaaggattt
     9121 caacggacga ttgctgtcgc tgcgaaccgc tgctgtctta cggctgttat caaaaaggga
     9181 atcaaccgat tcctgaagca tgcgcttttc gttgcgcaaa atcacctcag gagcgcggat
     9241 ttcgatcaat ttacgtaatc ggttattacg tatgatcaca cgacgatata aatcattcag
     9301 atcgcttgtc gcaaaacgtc cgccttcaag cggtacgagc gggcgcaatt cgggaggaat
     9361 gaccggcact acatcgagca ccatccactc agggcggttt tcgatcgcgc cttcgctctg
     9421 gcgaaatgca tcggccacgc gaagctgacg caacgcatcg gaacgttttt gaatggatgt
     9481 cgaaggatcg cgcaacactt tacgtaagtc aatgcatatc tgctcgacat caacacgctt
     9541 gagcaattct ttgacggctt ccccgccgat caacgcaata aacttattgg gatcggtgtt
     9601 cggcagattt tcattatcca atccgagctc ttctttaaca cgatacagct cttcttcagg
     9661 gataatatct ttgtacttga gaccgctttt gccgggctga acgacaatat agttttcgta
     9721 ataaatgatt ttttcaagct gcttgatgct caggccaaga agcgaaccga tcttgctcgg
     9781 caaggatttg aaaaaccaaa tgtgtacgac gggtacggcc aacgaaatat gccccatgcg
     9841 ttcgcgacga acggctttga gcgtcacttc cacgccgcaa cggtcgcaga tgatgccttt
     9901 gtagcgaata cgcttgtatt taccacaatg gcattcccaa tcctttacgg gaccgaaaat
     9961 tttctcgcaa aacaaaccat ctttttccgg cttgaatgag cggtaattga tagtttccgc
    10021 tttggtgact tctccgtacg aacggctcaa aatcgcatcc ggcgatgaga taccgatcgt
    10081 gatcgttgaa aagtcttttg acaaactgtt attggacaaa tatatggaca cggttgatcc
    10141 tcctttatcg gagcgttatg agttaatcta aattacaaca ccacgtgcgt tgcaattact
    10201 caatttttac ttcaaggcca aggccctgca attcgcgtac aagcacgtta aatgattcgg
    10261 gcacacccgg ttcaggcaaa ttatcgcctt taacgatagc ttcgtacact ttcgcacgtc
    10321 cgccgacatc atcggatttg tacgtgagaa tttcttgcag aatgtaagcc gcgccgtagg
    10381 cttcgagcgc ccacacttcc atttcaccaa aacgctgacc gccaaactga gccttacctc
    10441 ccaagggctg ttgcgtgatg agtgagtacg gtccgatgga acggctatgg atcttatcat
    10501 cgacaaggtg gtttagtttc atcatgtaga tgtagccgac catggtttcc tgatcgaagc
    10561 gatcaccggt acgaccgtct gtcaagcgca ctttaccgtg atccggtaaa ccggccagac
    10621 gtaattcgtt tttcacatcg gatatcttcg ctccgtcaaa cacattggtt tcgtagtgtt
    10681 tgccgagaat cttaccagcc caaccaagcg aagtttcaaa tatctgtcca agattcatac
    10741 gcgaaggcac accgagcgga ttgagaatca cgtcaacagg cgtaccgtcg ctcagaaatg
    10801 gcatatcttc ggtgggaacg atttttgaca cgacgccttt gtttccgtga cgtccggcca
    10861 tcttatcgcc gaccgagatt ttgcgtttct tagcaacttg gactttagcc aactgtacaa
    10921 ttcccggagg caactcatcc ccaacctgga ctttgaattt ctcggaattg tattccgttt
    10981 cgacttcttc ggcattgcgt tcaaagttgt caaataagaa attggccatc tcgtttgttt
    11041 cttcatcatc cgtccaacgg ctttcctgca ggttgatttc cgcagggtcc aaatttttga
    11101 aagaatcttc ggtaaatttt ttaccttttt tcaaaaattc agaaccgtcc gtgtaaacaa
    11161 cgcctgccga tactttgccg acaagcagtt tggtcaactc atcgcgcaag ttggatttca
    11221 gacggcgaac ttgtttttta agttcgtttt caagttcttc gagcttcttc ttatcttctt
    11281 ttttggactc agtatctttt ttcttacggc tgaaaagttt ggtcgaaatg acaattccct
    11341 tcatgcccgg aggcgctttg agggatgcat ccttgacgtc accggcttta tctccgaaaa
    11401 tcgctttcag aagtttttct tccggcgtag gatccgtttc acctttaggt gttacttttc
    11461 cgacaagaat atcaccggcc aatacttctg caccgacacg gataacaccg ttttcatcaa
    11521 gatccttcgt cgcttcttcg ctgacgttgg gaatttcgcg cgtaagttct tcttcgccgc
    11581 gtttggtatc acgtacttgt atttcgtact cttcgatgtg tattgacgta aatacgtcat
    11641 cacggatcac gcgttcgctc agaatgatag catcttcgaa gttataaccg cgccacggca
    11701 tgaaggccgc caacacgtta cgaccgagag caagatcgcc gcgttcggtc gaacaaccgt
    11761 cagcaataat ttgtccggct ttcacttcat cacctacaga gacgatggga cgttgattga
    11821 tacacgtatc ttgattggta cggaaaaact tcagtaaccg atacgtcatc gtatccgaaa
    11881 cagagtcttt acctttcttt ccacccatgg ttttcttacg gatggtgatt tctgttgccg
    11941 aaacatcttc gatcacaccg tcaatatccg aaatgataac cgaacgtgaa tccacagcca
    12001 cggtgcgctc aagaccggta cctacgatcg gagcttccgg cacaagcaac ggtaccgctt
    12061 gacgttgcat gttagatccc atcagtgcgc ggttggcatc atcgtgctca aggaatggaa
    12121 tcaaagcggc cgccggcgat acaatctgcg caggcgaaat atccatgaac tggatttcat
    12181 ccggagttac caccggaaaa tcaccttctt tacgcgcttt tacgaattca ttgtcaaaag
    12241 aacctttttt gtcgatcggt tcgttcgctt gcgcgatcgt catgccgtct tcgtcgctgg
    12301 caatcagaaa ttcaatattt tcctgtacac gacctttgac gactttacga tagggcgttt
    12361 caataaaacc gtaccgattc actcgtgcaa aaaggcaaag cgaagaaata agaccgatat
    12421 tcggtccttc cggtgtttcg accgggcaaa gacgaccgta gtgcgtatag tgcacgtcac
    12481 gcacttcaaa tcctgcgcgt tcacgcgtaa gaccgcccgg tccgagagcc gagagacgac
    12541 gtttatgcgt caactcggat aacgggttcg tctgatccat aaactgtgaa agctggctgg
    12601 taccgaaaaa cgtattgatt acgctggata ccgtacgcgc attgacgaga tcttgtggtg
    12661 tcagattttc attatcgcgc aggttcatac gttctttgat cgtacgtgtc atacgcgtca
    12721 aagctacgct gaattgtccg gcgagctgct caccgacggt tttcacacga cgatttccaa
    12781 gatggtctat atcgtcggtt tggcgaatac cgtcgcgcaa ttctatcaat gctttgatga
    12841 tggcgataat atcatcttta gtaagaaccg taaccgattc cggtatattg aggctcagac
    12901 gtttattgat acgattacgt cctacatcac cgagatcata acgtttggaa ttaaaaaatt
    12961 gacgttccaa aagtccacgt gccgtttcca aatccggtgc atcgccggag cgaagttgac
    13021 ggtaaatcgt ttccaaagct tctttttcat ctttggtaac gtctttacga atcgtattgg
    13081 cgatcacttc cggaatgact tcttcggctt tgatcaaatc aatttttttg attttgctgc
    13141 gtttgagctt ggcaaaagtc tcttcggtca gttcggtttc gcgttcggca ataatttcgc
    13201 cggtttcctc attgacgata tctaacgcca acttacggcc catgatctta tcgccgtcaa
    13261 tcttggagat ttccacttcg tcaataatat taaaaagctt gagcatgtcg cggtccgacg
    13321 aatagcccaa agcacgtaaa aacgtcgtta cggggaattt ctttttacgg tcaataaaca
    13381 catacatgac atcgttgatg tccgtcgtga actctatcca cgaaccgcgg aaaggtatga
    13441 cgcgcgccga aaacatcttg gttccgttcg gatgcagcga ttcgtcaaaa aatacgcccg
    13501 gggaacggtg aagctggctt acgatgacgc gttctgcacc gttgataaca aaggtaccct
    13561 tctccgttat gtacggaagg ttaccgagat atacatcctg ttcgatgatc tcgggatgct
    13621 tgtcatccgc tgattttttg ctgatcagac gcagcgtcgc tttcaaagga acagaaaacg
    13681 aaacaccgcg ttcctggcat tcgcgtacgt catatttagg acgttcgacg taatacttca
    13741 aaaattcaag tttgaacaat tcttttgcat cgtctaccgg aaaattgctc aaaaatacat
    13801 tctgaagacc ggt
//
LOCUS       DAOQGF010000094        13549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2111_length_13549_cov_7.150511, whole genome shotgun sequence.
ACCESSION   DAOQGF010000094 DAOQGF010000000
VERSION     DAOQGF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2111_length_13549_cov_7.150511"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PK937_11965"
     CDS             <1..744
                     /locus_tag="PK937_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNH33332.1"
                     /translation="HPGLFWGNNPRAQGKNYKILGMPDQGTLAEYVTVPVENLAEAPG
                     HLSDEEAAALPLAGLTAYRALMKQGKADAGETVVITGIGGGVAALTLQMAMTVGAQVV
                     VTSGHDEKIARALSLGASAGYNYKATDMFKKMASDGIEPDLIVDGTGGASFGEWLNLM
                     KPGGRYVTYGATNGNPEHIDLRKIFWKQLTIQGSTMGTIDDFRAMIKIYDQYRLKPAV
                     DKVFSLAECRSAFHRMESSEQFGKIILKP"
     gene            753..938
                     /locus_tag="PK937_11970"
     CDS             753..938
                     /locus_tag="PK937_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33333.1"
                     /translation="MKKTLGQIAMHVTHLDVMEDFYKNTVGLTHLFTVPNRLSFFDLG
                     GVERPKNILFDFMCVIY"
     gene            1217..1510
                     /locus_tag="PK937_11975"
     CDS             1217..1510
                     /locus_tag="PK937_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33334.1"
                     /translation="MVEAVINLFSKKWNKKRKKNITKFRAGSLRIKSNIFFQLQCIQL
                     NKNTQCFVLKLEDFMEIKAEKLETKNPTEMSVGDWLILIGAGMMVAGGFMQQS"
     gene            1536..1658
                     /locus_tag="PK937_11980"
     CDS             1536..1658
                     /locus_tag="PK937_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33335.1"
                     /translation="MEIKAEKLETKNTTELSVADYLILGGGLMIAAGYVYNQVS"
     gene            1734..4748
                     /locus_tag="PK937_11985"
     CDS             1734..4748
                     /locus_tag="PK937_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH33336.1"
                     /translation="MKLNRLTILILFLPIFTIFSQFANSGSNAFQQSVRYEIDLNLNI
                     SENIIVAEEKLFYRNNSPDTLEYVWFHLYPNAYKNENSIFAREMAERGVNDFFYTSEK
                     DRGYIEIKKISVNNLALTTRFKENDETELKVNLDKSLSPGDSVIFEIQFKVKIPSFIS
                     RMGQKDLRYEITQWYPKIAVYDNNGWHNDGYHFLGEFYGEYGDYKVSFNVPEWMWVAA
                     TGENIDKDEIAKINDMVLFTSKIDSLPKKLKRLKLDSIVTVQKEIKKCLLAGKFKNKK
                     ITFVAQNVHDFAWACDYRYLIKESFYENTKILTYVTPKHYIGWEYSNDYASDALRYMS
                     NWYGDYIYPVLNVVESNGPYGGMEYPNLVLITSRGDGLLRTFEEVIFHEIAHQWFYGM
                     LGNNEINDAWLDEGLTMFSEMRYFREKYKGSFSNSKFLNLLGLDQITEHSELMKANLY
                     FISGFDYIPPASEPSPNYRSRLEFSNVYVRTAFTLDMLKYELGDSLFNTCMKEYYKRF
                     LLKHVSPASLINTFNDVTSSSWDWYFRQWIYTQEQLNLKAGKLESYSQSENQFETSFK
                     IIKESKLKTNCKIEFTTKQNERFVERIFIEQPETEIRVNSKDEIISARIDPHNELLEF
                     NRRDNSTNYSGFRLKSPISTPSFDKYTLMVSPLANYTDYSKWGLGGTIWYFDFPTNLS
                     SIKGSQIAISALYYREIKILDYSANIVTTTDIFKRYNKIWGTNYKLRGEKLTSVGFEY
                     RFGNSRFENPSYNLSVELKNSNLFEKRYYKSYQAIEGKDIKVVINNTFGLDYRLYSGL
                     FNFGFQKGMKISGYSNYKYTKSYLEFYQNVPITRLISVENSINSGFKTGSSPLQENLF
                     LSPNSNLISTDMGVAFGFKYLPNQNFNGKMLGYINQNLTGNEIFTSRSEVNFNLFKVG
                     HEPFFLKMFGEIGKFWNSENQHQTNKQNFIDSGFGLRFIGYSLMVVVWKNYNVRKVNG
                     EHFLRKNREAKFEGIFITLDLGYLFRVGIQYK"
     gene            4748..5878
                     /locus_tag="PK937_11990"
     CDS             4748..5878
                     /locus_tag="PK937_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33337.1"
                     /translation="MINLNINAETKIETLPLTFSNIDGSWVVGSFYKGTSYLKTSVMG
                     VEAIKLLNQNMNILNVERVLSNRNQDNIKIQQLVSALYDSNLIHKIDSKIISNPELMT
                     NGKFLSELAKHLFSNFALLVYVGLVVAGSISIVLNSDILLLPKQIQTFGFFISSLLIG
                     WLLTLKHEWFHYLAAVSVGVPAHIKFGTRFFFVVLETDVSSLYILETKKRYRVYLAGI
                     CGDLVVCSIFSLLKNFNLSELNVLLDFALISSFGGILFQFNFFLKTDLYFVIADGFHL
                     NNLYMNSDMLIKSWLGFQFKNVSFSKSVFLFSVLRIINYMILSLAVVLVIIGLFLHQV
                     ENPISVESNQFSYVAMVLYFIVFLIVKYGNRKKHKTQLLFES"
     gene            5885..7108
                     /locus_tag="PK937_11995"
     CDS             5885..7108
                     /locus_tag="PK937_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH33338.1"
                     /translation="MGNNSRFFDETDCFRLVPYHIRKDGDDFLVIKKGKFEIIKTSAV
                     GVEVLKLLGKGNRIIDVKQWISEKYKIEISLVKIDSFIELLWESKFIYALNGELSNEI
                     KANSLLNSIASLKASILTRVLYICYHKLSIKLSYRILKLLLLKKIPAPSNGSIKSAYE
                     ETNIRTFDAKAFCNKNRQIQRQILFDRILFFSLSYKNLDTWLNNYFKVENIDVINKAL
                     SFGKGAILCGFHSGSYNIIPFAMAKRGVKIHSLAIFEDVMYNQIIKRIEQVNREAFPL
                     NAVIHNRSGNDGLKLFRLLKNKATILLYCDTHVIIGDGGVLIDFLGKKLKVNKGIPFL
                     HKRTEAPIIPVMSYFENSCNKIVCLNPIMINDTSTLSDKQVLQNIFLDFEHFIRKKPE
                     QWSKWVNLNEMIVKQ"
     gene            7165..7539
                     /locus_tag="PK937_12000"
     CDS             7165..7539
                     /locus_tag="PK937_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH33339.1"
                     /translation="MKKTLGQIAMHVTHLDVMEDFYKNTVGLTHLFTVPNRLSFFDLG
                     GVRLMLGIAEPGEYALSRPILYYKTSHLDEEFARLKSVGVVVVHQPQLVAPMPDHDLW
                     MAFFKDPEDNTLGIMEERSKNK"
     gene            7617..8345
                     /locus_tag="PK937_12005"
     CDS             7617..8345
                     /locus_tag="PK937_12005"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNH33340.1"
                     /translation="METMTEPKPLGALDRIIGVITEPEKTFTDIAVRPSWGLPMMLAL
                     LAMIAVGILMKDVIQKESAEKTRISIMENKNIPDGDKQRIIDQSIEMMKKFWVVGFAV
                     GMVVIVIAYFISALILKLGGSAVLKSTATFTQVLTIFSWSGIVDLLGALIKLPIMYIN
                     QSMRVDTGLGILIGEELGHTTTYLIMSKFDIFTIWMLIVMSIGIAVVFKAPKSKSMIL
                     VFGLWLVWVLAQWGMALMGMRVGG"
     gene            8581..9900
                     /locus_tag="PK937_12010"
     CDS             8581..9900
                     /locus_tag="PK937_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH33341.1"
                     /translation="MKHLLTIVWIALAANLTAQQKQALTIEEALTIAMKNNVQVISAQ
                     NDYNSASANVLPKTVGKILPTVDASMRASRTMMNTLSGTSPLTNRDTTTGSASNSYSY
                     SLTANYVLFDGFRNWTEASQARLDESAARYNFERTRQSVTLDVYEAYINVLKNQQLLR
                     INEENLKRSEEQLKRLEERNKLGAQILSDVYKQRVQVGSDKLSVSKSKNNLKTSKASL
                     ASLIGIDVNSDLELKELIIASSLDVNQMNFDEAYGQALQNRKDYLASESRVQSARRTV
                     SIARSGFYPTVNAFASYQWGDKNLKFANYSNNDNTSIGLSVSIPIFSGFETNSRVIQQ
                     DQSLQTARSNAESTKRKVALDVKIALLNMQTAYDNMKLSEENVKAAKEDLRLATEKYN
                     LGAGTILDQITANASYAAAEANYTQSAYDFVYAKQQYLLAVGTLDVQ"
     gene            9936..11231
                     /locus_tag="PK937_12015"
     CDS             9936..11231
                     /locus_tag="PK937_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH33342.1"
                     /translation="MSKRNKILIGVGLTLVLTVVIVLNIRGKKEDAVEVQTEKVKRGD
                     VIKTVSASGKIQPVTDIKISSNVSAKILQLTVKEGDKVKRGQVLVSLDRTFYDAQLDQ
                     AKAELLRAKSQVATAQANYEKAQQDFSRVKDLTAKQLASASELEVAQTTLSTTRASVE
                     SAESQVQAAEASAQQAADNLSKTTIYAPIDGTISKLNKKVGEIALGSQFSQDVIMIVA
                     NLNEMEARVNVDENDVVAVEIGDTAVIEVDALTEQKLRGRVTEIANSATVKATGTSEE
                     KTEFEVKIALDDTPLMLRPGMSTTSDITTDVRGNTLKVPIQCVTVREPERLKRKEGVE
                     KEGAAMASDTNTQFVPNKDGVVEIVFVIENGMAVARPVKTGIQSESHIEILDGIKEND
                     EVVVGNFRAISKILENGSKVKVDNEKKPNKDDNKEARDN"
     gene            11262..11987
                     /locus_tag="PK937_12020"
     CDS             11262..11987
                     /locus_tag="PK937_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH33343.1"
                     /translation="MLIDIRNITKRYQIGTEEVHALRGVSLQIKKNDYIALMGPSGSG
                     KSTMMNVIGCLDTPTSGEYLFEGQDVAQMNDDQLAEIRNRKIGFVFQNFNLIPRSDVF
                     HNVELPMIYAGHAPAERKRLTIEAIEKVGLGARMKHKPNELSGGQRQRVAIARALVNN
                     PSIILADEPTGNLDSKTGEEIMQVFEQLHKAGNTIILVTHEEDIASHAHRVVRLKDGV
                     VETDEAITGRKVFKKVLVEAEAQ"
     gene            12003..13241
                     /locus_tag="PK937_12025"
     CDS             12003..13241
                     /locus_tag="PK937_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33344.1"
                     /translation="MKRFIYETLEAFRIAMTAIRANKARGFLTTLGIVIGITAVSTTM
                     TVMNGMKTSFQSQLSVLGNDVLYVTRQPWIQMDDWWKYRNRPQINLEEAERLEKSLGD
                     KVVAINPTVGTMRPVKYESKTLDDIFIKGTTDKELLTSSTTPEFGRYIGAIDVQNSKY
                     VCVIGAEVKKQLFENVDPIGRRIRVGAYNFRIVGVLEKQGKLLGNFGGPNFDNQVAIP
                     ITTFMKSFGRSRGLSLAVKVQSVSKLDETQDEIIGAMRKIRKLSPSRDDNFSVNRQDS
                     FLKIYDSIMGVVGLVGILITGISLFVGGIGVMNIMFVSVTERTKEIGIRKAIGAKRRT
                     ILIQFLFEAAMICLIGCAIALVLSYLLSVVIDTFFAASLSLGIVIVAIVMAIMVGIIS
                     GLLPALKASKLDPIDALRYE"
     gene            13274..>13549
                     /locus_tag="PK937_12030"
     CDS             13274..>13549
                     /locus_tag="PK937_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33345.1"
                     /translation="MKIDMRENLKMALNAVFANKLRSFLTLVGVVAGVASIIAVMTGI
                     AVIQGKMEADMSQLGSGTFQVQKWPAIGGPGQNWRKIMQRKPLTIEQA"
BASE COUNT         4239 a         2429 c         2946 g         3935 t
ORIGIN      
        1 catcccggtt tgttttgggg gaacaacccg agggcacagg gaaaaaatta caaaatcttg
       61 ggtatgcccg atcaaggtac gcttgcggag tatgtcacgg taccggtaga aaatttagcc
      121 gaagcgccgg ggcatctttc ggatgaagaa gcggcggcgt tgccgctggc tggtttgacg
      181 gcgtatcgtg cgcttatgaa acagggtaaa gccgatgcgg gcgaaacggt cgtgatcacg
      241 ggcatcggcg gcggcgtagc ggcattgact ctgcaaatgg caatgaccgt cggtgcgcag
      301 gtcgtcgtga cgtccggtca tgatgaaaaa atcgcacgcg cactttcgct cggtgcatcc
      361 gccggttata actataaagc aacagatatg ttcaaaaaga tggcttcgga cggtatcgaa
      421 cccgatctca tcgtggatgg caccggaggc gcatcatttg gcgaatggct caatctcatg
      481 aaaccgggcg gccgttatgt gacgtatggc gcaactaacg gtaatccgga acacatagat
      541 ttgcgaaaaa ttttttggaa acaactgact atacaaggct ccacgatggg aactatcgat
      601 gattttagag ctatgattaa gatctacgat caataccgac tgaagccggc tgtggataaa
      661 gtgttttctt tagccgaatg ccgttccgca tttcatcgta tggaatcaag tgaacagttt
      721 ggtaaaatta tccttaaacc gtaaagtttt gtatgaaaaa aacgctcggt caaatcgcaa
      781 tgcatgtgac ccatctggac gtaatggaag atttttacaa aaacacggtg ggcctgacgc
      841 atctgtttac tgttccgaac cggttatctt tttttgatct tggcggtgtt gagcgaccta
      901 agaatatatt gtttgatttt atgtgtgtaa tctattgaat aggataagta cgggggacta
      961 gtggcttgat tagaagattg tcgtgatata ttagaacgaa cacaaaatct tattgacatc
     1021 agggactggt gtgtctatat taaactgttc tataagggaa aatgggggtt gtgaatctta
     1081 gttagtagca atccaatcca atccaatcca atccaatcca atccaatcca atccaatcca
     1141 atccaatatc agtttggcgt tggaaggaat tcatgtttta tttatataaa ggaggtgagt
     1201 aaattcatcc tgaacaatgg ttgaagcagt aattaactta ttttcaaaaa aatggaacaa
     1261 aaaaaggaag aaaaatatta ccaaattcag agctgggagc ttaagaataa aaagtaatat
     1321 tttcttccaa ttgcaatgta ttcaattaaa taagaatacg caatgttttg ttcttaaact
     1381 ggaggatttt atggaaatca aagctgagaa attagaaaca aaaaatccca cggaaatgtc
     1441 tgttggtgat tggttgattt tgattggtgc cggcatgatg gttgccggtg gtttcatgca
     1501 acaaagttaa acttaaacta aaggaaattt gtatcatgga aattaaagct gaaaaactcg
     1561 aaacaaaaaa tacaactgaa ttatcagttg ccgattatct tattttaggt ggaggtttga
     1621 tgatagcggc tggctatgta tataaccaag taagttagtc acttctaaac gcaacggcag
     1681 gtgttgtttt aacaatacct gccttgcttt atttgaaaag caacgtaaat accatgaaat
     1741 taaataggtt gactatcctc atattgtttc ttccgatttt tacaatattt tcacaatttg
     1801 caaatagtgg aagtaacgct tttcaacaaa gtgtaagata tgaaatagat ttaaatttaa
     1861 atatttctga aaacatcatc gtagctgaag aaaagttatt ttatcgtaac aattcacccg
     1921 acacattaga gtacgtatgg tttcaccttt acccaaatgc atacaaaaac gagaattcta
     1981 tctttgcaag agaaatggca gagcggggag ttaacgattt tttttatact tcagaaaaag
     2041 atagaggata tattgaaata aaaaaaattt ccgtgaataa tctcgcttta acaacaagat
     2101 ttaaggagaa tgatgaaaca gagttaaaag taaatttaga taaatctcta tcccccggag
     2161 attcagttat ttttgaaata caattcaaag tcaaaatccc cagttttata tctcgtatgg
     2221 ggcaaaaaga tctgcgatat gagattactc agtggtaccc caaaattgca gtttatgata
     2281 ataatgggtg gcataacgat ggctatcatt ttctcggcga attttacggg gaatacggtg
     2341 attacaaagt ttcattcaat gtaccagaat ggatgtgggt tgccgctaca ggagaaaata
     2401 ttgacaaaga tgaaattgct aaaataaacg atatggtgtt gtttacctct aagatagatt
     2461 cacttcccaa aaaacttaaa cggttaaaac ttgattccat cgttacggta caaaaggaaa
     2521 taaaaaaatg tttgttagct ggtaaattta aaaacaagaa aataacattt gtagcgcaaa
     2581 atgtgcatga tttcgcttgg gcttgcgatt atcgttattt aattaaagaa tcgttctatg
     2641 aaaatacaaa aatattaacc tacgtaacgc ccaaacatta catcggttgg gaatattcaa
     2701 atgactatgc atctgacgcg ctccgataca tgtcaaactg gtatggtgat tatatttacc
     2761 ctgtattgaa tgttgtggaa agcaatggac cttatggtgg aatggagtat cctaacttag
     2821 ttttaatcac ttctcgtggt gatggcttat tgcgaacctt tgaggaagtg atatttcatg
     2881 aaattgcaca ccaatggttt tatggaatgt tgggtaacaa tgaaatcaat gacgcttggc
     2941 ttgatgaggg gttgaccatg ttttcagaaa tgcgttattt tagagaaaaa tataaaggtt
     3001 cattcagtaa ctcgaaattc ctgaatttat taggtcttga tcagatcaca gagcattctg
     3061 agctaatgaa agcgaattta tatttcatat ccggtttcga ctatattcct cctgcatctg
     3121 aaccttcacc taattacaga agcagattgg aatttagtaa tgtttatgtg cgtacggcat
     3181 ttactttaga tatgttaaag tatgaattag gcgacagcct ttttaatacg tgcatgaaag
     3241 aatattataa acgattcttg ctaaaacatg taagcccagc ctcgttaata aacacattta
     3301 atgatgtgac ttcttctagt tgggactggt attttaggca atggatttat acccaggagc
     3361 aacttaacct aaaagcgggg aaactagaat catactctca atcagaaaat caattcgaaa
     3421 cctcttttaa aattatcaaa gaaagtaaat tgaaaaccaa ttgtaaaata gagtttacaa
     3481 ctaaacaaaa tgaaagattt gttgaaagaa tatttattga gcaaccggaa actgaaattc
     3541 gtgtgaattc aaaagatgaa attattagcg caaggattga tcctcataat gaacttttag
     3601 aatttaatcg gagagataac tctactaatt attctggatt taggcttaag tctcctattt
     3661 ctacaccatc atttgataaa tatactctaa tggtttcgcc gcttgcaaat tatacggatt
     3721 atagtaaatg gggattaggt ggtacgattt ggtattttga ttttcccacc aacctgtcat
     3781 caatcaaagg aagtcagata gccatttcag ctttatatta tcgagaaata aaaattttgg
     3841 attattctgc taatattgta accacaacgg acatctttaa acgatataat aagatatggg
     3901 gtacgaatta taaattgcgt ggcgaaaaac tcacgagtgt tggttttgaa tatcgttttg
     3961 gaaattcccg atttgaaaat ccatcctaca acctatctgt tgagctaaaa aactctaatc
     4021 tttttgaaaa gagatattat aaatcgtatc aagctattga gggaaaagat attaaagtag
     4081 ttattaataa tactttcggt ttggattaca ggctttattc aggattgttc aactttggct
     4141 ttcaaaaagg aatgaaaatt tcaggatatt ctaattataa atatactaaa agttaccttg
     4201 agttttatca gaatgttcct ataaccagat tgataagtgt agagaactca ataaattccg
     4261 gctttaaaac aggatccagc ccacttcaag aaaacctgtt tctttcacca aattccaatt
     4321 tgataagcac ggatatgggc gttgcgttcg gttttaaata tttaccaaac caaaacttta
     4381 acggaaaaat gttgggatat ataaatcaaa atctgacagg taatgaaata tttacctcgc
     4441 gttcggaggt taattttaat ctatttaaag tgggacatga gccttttttc cttaaaatgt
     4501 ttggtgaaat aggcaagttt tggaattcag agaatcagca tcaaacaaac aaacaaaatt
     4561 tcatagattc aggttttggg ctccgtttta tcggttattc actaatggta gttgtatgga
     4621 aaaactataa tgttagaaaa gtaaatggag aacatttctt aagaaagaat cgcgaagcaa
     4681 aatttgaagg tatatttata acactcgatt taggctactt atttagagta gggattcaat
     4741 ataaataatg attaatttaa atataaatgc ggaaacaaaa atagaaactt tacctctaac
     4801 tttctcaaac attgacggtt cttgggttgt tggtagtttt tataaaggga catcatattt
     4861 gaaaacatca gtgatgggtg ttgaggctat caaactgcta aaccaaaata tgaatattct
     4921 aaatgtagag cgagttttat cgaatcgaaa tcaggataat attaaaatac agcaattggt
     4981 tagtgcatta tacgactcaa atctaattca taaaattgat agtaaaatta tatcaaaccc
     5041 tgaattaatg acaaacggga aattcctatc agaacttgcg aagcatttat tttcaaactt
     5101 tgcattgctt gtatatgtag ggttggtggt ggccggaagt atttcgatcg tacttaatag
     5161 tgacatattg ttgcttccaa aacaaattca aactttcggt ttttttattt catcgttatt
     5221 gattggctgg ttgttaacat tgaagcacga gtggtttcat tatcttgcag cggtgagtgt
     5281 tggtgttcct gcgcacataa agtttggcac taggtttttt tttgttgttc ttgaaacaga
     5341 tgtaagttcc ctttacatct tggaaacgaa aaaacgttac agagtttatt tagctggaat
     5401 ttgtggcgat ctggtcgtgt gttccatttt ttcgctgtta aaaaatttca acttatctga
     5461 attgaatgtc cttttggatt ttgcattgat ttcttcattt ggaggtattt tgtttcaatt
     5521 caattttttc ctcaaaacag atttatattt tgtaattgct gatggttttc accttaacaa
     5581 tctttacatg aattctgata tgttgattaa atcttggtta ggatttcaat tcaaaaatgt
     5641 ttctttttcc aagtcagttt ttcttttttc ggtattgcga ataataaatt acatgatact
     5701 ttctttggct gtcgtgctgg taatcattgg tttattttta catcaggtgg agaacccaat
     5761 ctctgttgaa tcaaatcaat tttcttatgt agcaatggta ctatatttta tagtgttttt
     5821 gatcgtaaaa tatggcaata gaaaaaaaca caaaacacaa ctactttttg aaagttagat
     5881 tagattgggc aataattcaa gattctttga tgaaacagat tgttttagat tagttcctta
     5941 tcacatcaga aaagatggtg atgattttct tgtcataaaa aaaggcaaat ttgaaatcat
     6001 caagacctct gctgtagggg ttgaagtgct gaagctactg ggcaagggaa atcggataat
     6061 tgatgttaaa cagtggattt ctgaaaagta taaaattgaa atatctttag ttaagattga
     6121 ttcttttatt gaacttctat gggagagtaa attcatttac gctttaaatg gagagctttc
     6181 gaatgaaatc aaagctaatt cgttgttgaa ttccattgct tcgcttaaag catcaatatt
     6241 gacgcgcgtt ttgtatatct gctatcacaa actttcaata aaattatcgt atagaatatt
     6301 aaaattacta ttactgaaaa agatacccgc gccgtcgaat ggtagtataa agtctgctta
     6361 tgaggaaaca aatatacgta cattcgacgc aaaagctttt tgtaacaaaa acagacaaat
     6421 acaaaggcaa attctttttg ataggatatt gtttttttca ctgagttata aaaatttaga
     6481 tacatggtta aacaattatt ttaaagtcga aaacattgat gtcatcaata aagcattatc
     6541 tttcgggaaa ggtgcgattt tatgtggatt tcattccggt tcatacaata taattccttt
     6601 tgcgatggcc aaaagaggcg ttaagattca ttcgttagct atatttgaag atgtgatgta
     6661 taatcaaatt attaagcgca ttgagcaagt caatcgtgaa gcctttccac taaacgcagt
     6721 tattcataat cgaagtggta atgacggctt aaaactattt aggctattga aaaataaagc
     6781 tacaatactt ttatactgcg acactcatgt aattattgga gacggtggtg ttttaattga
     6841 tttcttaggt aagaaattaa aagtgaacaa aggtattccc ttccttcaca aaagaacgga
     6901 agcacccatt attcctgtaa tgtcatattt tgagaattca tgcaataaaa tagtttgcct
     6961 aaacccaata atgattaatg atacaagcac gttgagtgat aaacaggtgc ttcaaaatat
     7021 ttttttagat ttcgagcact tcataaggaa aaaacctgag cagtggtcga agtgggtaaa
     7081 cttaaatgaa atgattgtaa agcaataatt tgtattcatc tatgaacagt ttggtaaaat
     7141 tatccttaaa ccgtaaagtt ttgtatgaaa aaaacgctcg gtcaaatcgc aatgcatgtg
     7201 acccatctgg acgtaatgga agatttttac aaaaacacgg tgggcctgac gcatctgttt
     7261 actgttccga accggttatc tttttttgat cttggcggtg ttcgcctgat gttgggaatt
     7321 gctgagcccg gtgaatacgc tctgtcccga cctattctat attataaaac atctcacctt
     7381 gatgaggaat ttgcacgtct aaagtccgtg ggcgtggttg ttgttcatca gcctcaattg
     7441 gtagcgccga tgccggatca tgatctttgg atggcttttt tcaaggatcc ggaagacaat
     7501 accttaggga ttatggaaga aaggtcaaaa aataaatgaa acctgtaaga aaaagtaatt
     7561 tgcggcaacg tatccgatag taaataaaaa ccatcccttt gttatcagga gatactatgg
     7621 agacaatgac agaacctaaa ccgttaggtg ctttggaccg gataatcggc gtgatcaccg
     7681 agccggaaaa gacatttacc gacatagctg tacggccctc atggggtttg cccatgatgc
     7741 ttgctttgct ggccatgatc gcagttggta tattgatgaa ggatgtgatc caaaaagaaa
     7801 gcgccgaaaa aacgcgcatt tccattatgg aaaataaaaa tatacctgac ggcgacaaac
     7861 aacgcattat tgatcagtct atcgagatga tgaaaaaatt ttgggtcgta ggtttcgctg
     7921 tgggtatggt cgttatcgtt attgcatatt ttatctcggc gttgatactc aagttgggcg
     7981 ggagcgctgt gcttaagtca acggctactt ttacgcaagt gctgactatt ttctcctggt
     8041 cggggatcgt ggatcttttg ggcgctctga tcaagctgcc gatcatgtat atcaatcaaa
     8101 gcatgcgcgt ggataccgga ctaggcatcc tcatcggaga agaactcggt catacgacga
     8161 cctacttgat catgagtaaa tttgatatct ttaccatatg gatgttgatc gtgatgtcta
     8221 taggtatagc tgtagtgttc aaagcgccta aatcaaaatc gatgattttg gtttttggtt
     8281 tgtggttggt ctgggtattg gcacagtggg gtatggcgct gatgggtatg cgtgtcggag
     8341 gttaattttt tataaattct atgatctaat gtgtatagcc gttttcttat ggtaaagaag
     8401 ccataataaa acggcttttg tatgtgcaac attttttgtc tgaaacagtc taatcggtat
     8461 aagaaatacc gttaaacgaa aaaaaggata atttttcggg aaaaagtgaa acattttgat
     8521 aaattcgccg aatactcgat tgtttgtatg aattaccgaa ttttgaggag aataggagac
     8581 atgaaacatt tattgaccat tgtttggatc gccttagccg ccaatcttac ggcacagcaa
     8641 aaacaagcgc tcacgattga agaagcgttg accatcgcga tgaaaaacaa cgtacaggtc
     8701 atttccgcac aaaacgacta caattccgca tccgctaacg tattgcctaa aactgtaggg
     8761 aaaattttac cgacggttga tgcgtcaatg cgcgcgagtc gcacaatgat gaatacactt
     8821 tcaggtacaa gccccctgac gaatcgtgat accacgaccg gcagtgcttc taatagctat
     8881 agttactcgt taactgccaa ttacgttttg tttgatggtt ttcgtaattg gacagaagca
     8941 tcacaagcac gtctggacga atcggcggca cgatacaatt ttgaacgtac gcgtcaaagc
     9001 gtgacgttgg atgtgtatga agcgtatatc aatgtcctta aaaaccagca attgcttcgc
     9061 atcaatgaag aaaatctcaa gcgtagcgaa gaacaactca agcgtcttga agaacgcaat
     9121 aaattaggcg cgcagatttt gagcgatgtg tacaaacaac gtgtgcaggt cggctcggat
     9181 aagctatctg tcagcaaatc caaaaataac ctcaagactt ccaaagcttc attggctagt
     9241 ctgatcggta ttgatgtcaa ttccgatctc gagttaaaag agctaattat tgcctcaagt
     9301 cttgacgtga atcagatgaa tttcgatgaa gcgtatggac aagcgcttca aaatcgtaag
     9361 gattatttag cctccgaaag tcgtgtccaa agtgcgcgtc gcaccgtaag tattgcacga
     9421 agcggttttt atcctacggt caatgctttt gcgtcgtatc aatggggtga taagaattta
     9481 aaatttgcaa attacagtaa taacgataac acctcaatag gtttaagtgt ttccattccc
     9541 atttttagtg gatttgaaac caatagccgc gtgattcaac aagatcaatc gttgcagaca
     9601 gcgcgcagta atgccgagag caccaaacgt aaagttgcct tggacgtgaa aatcgctctg
     9661 ctcaatatgc agaccgccta cgataacatg aagttatccg aggaaaacgt caaagcggcc
     9721 aaagaagatc ttcgtttggc tacagaaaaa tacaatctcg gggcgggcac gattttggat
     9781 cagattaccg ccaacgctag ttatgcagcc gccgaagcca actataccca gtctgcttat
     9841 gattttgtgt atgccaaaca acagtacttg ctggccgttg gcacactgga cgttcaataa
     9901 gtaattttga gaaacccttt ttaggagata gagctatgtc taaacgtaac aaaattttga
     9961 tcggcgtcgg tttaacgctg gtgcttacgg tagtgatcgt tctcaacatt cgcggtaaaa
    10021 aagaagatgc ggtcgaagtg cagactgaaa aagtcaaacg cggcgatgtg atcaaaaccg
    10081 tcagtgcctc cggtaaaatc cagccggtca cggatatcaa gatcagctcc aatgttagtg
    10141 ctaaaatatt acaacttacc gtaaaagaag gcgataaagt gaaacgcggc caagtgctcg
    10201 tgtcattaga tcggaccttt tacgatgcgc aattagatca ggccaaagcc gagcttttga
    10261 gggccaagtc gcaagtggca acagcccaag ccaattatga aaaagcgcag caggattttt
    10321 cacgcgtcaa agatctaacc gccaaacaat tagcgtcggc ttcagaattg gaagtggcac
    10381 aaacaacact ttcgactacc cgcgcttcgg tcgaatcggc cgagagccag gtacaggcag
    10441 ccgaagccag cgcgcaacaa gcagcggata atctcagcaa aacgacgatt tatgcaccca
    10501 tcgatggtac gatcagcaaa ctcaataaaa aagtcggtga aattgcactc ggctcgcaat
    10561 tttcgcaaga tgtgatcatg atcgttgcca atctcaatga aatggaagca cgcgtcaatg
    10621 tagatgaaaa tgatgttgtg gccgtcgaaa tcggcgatac ggccgtgatc gaagtggatg
    10681 ccttgaccga acaaaaactg cgcggacgtg tgacggaaat cgcgaactcc gcaaccgtaa
    10741 aagcgaccgg tacatcggaa gaaaaaaccg aatttgaagt gaagatcgcg ttggacgata
    10801 cgcctctgat gctgcgcccc ggtatgtcta cgacatcgga tattacgact gatgtacgcg
    10861 gtaatacgtt gaaagtaccg atccaatgcg tcacggtacg cgaacccgag cgcctcaaac
    10921 gtaaagaagg cgtcgaaaaa gaaggtgcgg ctatggccag cgataccaat acgcagttcg
    10981 tgcccaataa agacggtgtg gtagaaatcg tatttgtcat cgaaaacggt atggccgtag
    11041 cgcgtccggt taagacgggc attcagagcg aatcgcatat cgaaattctt gacggtatca
    11101 aagaaaacga tgaagtcgtc gtcggaaact tccgcgcgat cagtaagatt ttggagaacg
    11161 gttccaaagt caaagtggat aacgaaaaga agccgaataa agacgataac aaagaagcac
    11221 gcgataatta atatacatcg gacgcttttg aggaaaacaa tatgttgatt gatatacgta
    11281 atattaccaa gcgttaccaa atcggaaccg aagaagtgca tgcactgcgt ggtgtgtcgc
    11341 tgcaaatcaa aaaaaacgat tatatcgcgc tcatgggacc ttcgggttcc ggtaaatcca
    11401 ccatgatgaa tgttatcggt tgtttggata cgccgaccag cggtgagtat cttttcgaag
    11461 ggcaagatgt cgcgcagatg aatgatgatc agctggcgga gattcgtaat cgcaaaatcg
    11521 gatttgtatt ccaaaatttt aatctcatac cgcgttcgga tgtgtttcac aatgtagaat
    11581 tgccgatgat ctatgcgggc catgcgccgg cggaacgcaa gcgtttgacg atcgaagcca
    11641 tcgaaaaagt agggctcggt gcgcgtatga aacataaacc caacgaactt tccggtggtc
    11701 aacgccaacg cgttgctatc gcacgtgcat tggtcaataa tccttcgatc atacttgcgg
    11761 acgaaccgac gggtaatttg gattccaaaa ccggcgaaga aatcatgcaa gtgtttgagc
    11821 agcttcacaa agccggcaat acgatcattc tggtcaccca cgaagaggat atcgcatccc
    11881 atgcgcatcg cgtcgtccgt cttaaagacg gtgttgtcga aaccgatgaa gcgatcacgg
    11941 gccgcaaggt tttcaaaaaa gtacttgtgg aagcggaagc ccaataacct tcgagaatta
    12001 ttatgaaacg atttatatac gaaacactcg aggctttccg tatcgccatg acggctatac
    12061 gtgccaataa agcccgcggc tttttgacca cgctcggcat agtgatcggt attacggccg
    12121 tttcgacgac gatgaccgtg atgaacggaa tgaaaacatc gtttcaatcg caattatccg
    12181 ttttaggaaa tgacgtgctg tatgttacgc gtcagccgtg gatacagatg gatgactggt
    12241 ggaaatatcg caatcgtccg caaatcaatc tcgaagaagc cgaacgcttg gaaaaatcac
    12301 ttggcgacaa agtcgtggcg attaatccga cggtgggtac aatgcgtccc gtcaaatatg
    12361 aatccaagac gctcgatgat atttttatca aggggacaac ggataaagaa ttacttactt
    12421 cgtccactac acctgaattc ggtcgttata tcggcgcgat tgatgtacaa aattccaagt
    12481 acgtatgcgt gatcggtgcc gaagtgaaaa agcaactctt tgagaatgta gatcccatcg
    12541 gacgtcgcat acgcgtcggt gcatacaatt tccgtattgt cggcgtattg gaaaaacaag
    12601 gaaaactcct gggtaatttt ggcggcccta attttgataa ccaagtggcc ataccgatta
    12661 cgacgtttat gaaatcgttc ggtcgctcgc gtggtttgag tctcgctgtc aaagtgcagt
    12721 ccgtcagtaa attagatgaa acgcaggatg aaatcatcgg tgcgatgcgc aaaattcgta
    12781 agctatcacc gtcgcgcgat gataattttt ccgtcaatcg tcaggatagt tttttgaaaa
    12841 tctatgacag catcatgggc gttgtgggtt tggtcggtat tttgatcacg ggtatttcgc
    12901 tgtttgtcgg aggtatcggc gtgatgaata ttatgtttgt atcggtgacc gaacgaacca
    12961 aagaaatcgg cattcgcaaa gccattggtg ccaagcgacg tacgatattg atccagttct
    13021 tatttgaggc cgccatgatt tgtttgatcg gctgtgcgat tgctcttgta ttgtcgtacc
    13081 tgctcagtgt cgtgatcgat actttttttg ccgcgtcatt gtccctcggt atcgtgattg
    13141 tggctattgt gatggcgatc atggtaggta ttatctcagg acttctgccg gcgctcaaag
    13201 cttcgaaact ggatccgatt gatgcgttgc gttacgaata aaaaaggaat gaatttcagg
    13261 aacagaggtc gctatgaaaa ttgatatgcg tgaaaatctg aagatggcgc ttaatgcggt
    13321 atttgccaat aaacttcgtt cctttctgac gctcgtcggt gttgtagccg gcgtagcatc
    13381 tatcattgca gttatgacgg gcatcgctgt gatacaaggt aaaatggaag ccgatatgag
    13441 ccaattggga tcgggtacct ttcaggttca gaagtggcct gcgatcggtg gccccgggca
    13501 aaattggcgt aaaatcatgc aacgtaaacc gctcacaatc gaacaagcc
//
LOCUS       DAOQGF010000095        12856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2265_length_12856_cov_6.432310, whole genome shotgun sequence.
ACCESSION   DAOQGF010000095 DAOQGF010000000
VERSION     DAOQGF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_12856_cov_6.432310"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PK937_12035"
     CDS             <1..237
                     /locus_tag="PK937_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNH33346.1"
                     /translation="GTLLTKNISIIGSTLRNKSHEEKSDLTKSMRELIMPHFTSGLFR
                     PIIDSEYSWFDAEAAHMRMKSNLNIGKIILRIGV"
     gene            349..2625
                     /locus_tag="PK937_12040"
     CDS             349..2625
                     /locus_tag="PK937_12040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH33347.1"
                     /translation="MDPKNKLFRVALIFIPILFVIGVILNSFMFYAKIRFSAGDWGSE
                     LLSIAIIGLVLYVAFNYPRFESISITQKLRLSLFFVVTIYGVGLLMTLVTQPVFFTPR
                     YSGSYVLRAYTWSGVIVAHVIGFVAVLSLGSCLRILQTLIFYRRKKNTATFFHLFLLF
                     GALTALSTAITGRPLKYDPSSNHIITFNLLVITTIFLLINAGRVTWVTVLNRRQKFLT
                     FIGGLVFTLISAGLLGAEIGGGATYADMVYSYSRTAGSFLFLMTVFVFVYSISTTINT
                     LLHLPTAAIYDKKVREINSIYSLSRTINSLFDVEKIVISVTELVCDATGANACWLELA
                     NTKNPAFRTHFDFAAVRQREPFQVHFLEVSFRKFLSKHENARLIPSNDQHHHYYLTEP
                     TDWIINNKQPILINQVKRDKRTKDLKRTPIESLVGVPLITSDGMLGIIFAVKSTSFGF
                     DQDDIAIISALANQTTVAIENTRLVKESLEKERLAEELRIAHDVQMKLIPQEIPDIRH
                     DVSKMTLDIGATTVPANEVGGDYYDFVRLADWRCGIAVADVSGKGTSAAFYMAEIKGI
                     IQALAGIYSAPKDLLIAVNDVLFGTIDRKSFITLLYADVDISKSELRFARAGHCPLLH
                     MRDGVKTYVQPNGIGLGLDKGNLFRQAIEEKTIRIDDRDVFVMYSDGLVEARNAKDEE
                     YGEERLSKCLDGVEHLPAGVIKDKIIADVKRFVGSAKTHDDLTCVVFRLADLQKQVVP
                     VAPVTEAETGEITAVRRV"
     gene            2715..3071
                     /locus_tag="PK937_12045"
     CDS             2715..3071
                     /locus_tag="PK937_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH33348.1"
                     /translation="MNGFEVTRKDEQDITVLYLKGYLDAHTYPHFEGELQKLMEEKRY
                     RIVVDFQELSYISSAGLGVFMGFIETVRENQGDIKLCTMSTKVYKVFDLLGFPTIYQI
                     LKDPSEARDHFVQANK"
     gene            3089..3514
                     /locus_tag="PK937_12050"
     CDS             3089..3514
                     /locus_tag="PK937_12050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33349.1"
                     /translation="MSSVTTNSFNMAIPSRTDNLGIIREFITSIARRFGFNDDAIGEI
                     ELAVDEACANVIKHAYKYDESKNIDITVETNGSKFTIIISDEGEGFDPDRLETPQQRL
                     QKHARGGLGIALIKRMMDEVVFDIHPGEKNRVKMIKYVS"
     gene            3533..5161
                     /locus_tag="PK937_12055"
     CDS             3533..5161
                     /locus_tag="PK937_12055"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNH33350.1"
                     /translation="MTFTSLTLESLQTTSSLILPFFLLYPEIHYRFRFFPFSRYFKKE
                     PEIIADIPHRIERGRRMPVFVLVKDAHLYPTSILSVRTIITKGANSDELIFQVQEEIR
                     QHWWWKKWDIAVNESWIGHTVQLRIVVEYRTAGVTHTVEADNIIGTTKAPFDVYVSGD
                     HWPQPQGWFNGDLHVHSDYTEDAIEYGAPLPMLRAASEALGLNFIAVTDHSYDLDNKK
                     EDYNQSDPTLAQWRTFIESVKQMNEESSSKVLLLAGEELSCMNIADENIHALILNSDH
                     FFYGSGDGGKNPLNTSSEHTVSGMARLLNKEALIMAAHPLEKPSPMERAFLQRGIWRL
                     EDFYNRSAEVLQILNGSNDTAFKQGLGLWVHLLLSGRRVYIVAGNDAHGNFNRNRQVR
                     IPFWTIEDISHHKQLGWARTVLYLGEKKPNTQNTIDAIREGCAIISTGPYVVFNVLNE
                     AGFSSRIGQNAKGRRFRVAWEASSTQEFGALETISVFQGVIGESHEKVSTMTVESWCD
                     QGEFHTTADAPSYIRLSVTTKSQNFCYTNPIWLE"
     gene            5231..6538
                     /gene="aroA"
                     /locus_tag="PK937_12060"
     CDS             5231..6538
                     /gene="aroA"
                     /locus_tag="PK937_12060"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNH33351.1"
                     /translation="MATALIKPATKIRIDCRIPGDKSITHRAVIFSAMSESIVSLFNI
                     ATGADVQSTIDCMRRLGVKVDQNGSELHITGCGMHGFVAPSAPLYCGNSGTTARLLAG
                     ILSGQSFKSELSGDASLSRRPMRRIIEPLTQMGAKIKTSAHQTLPIEIEGTELKAIDY
                     SLPIASAQVKSCILLAGHYAKGITTVRESVMTRDHTERLLPTIKKSVEDGRISLSVTG
                     GESFRFDHMTIPGDLSSAAFLIATGLLLPESEVTIRHVSLNPTRIAFLEILKKMGADI
                     TIHNITEAREPYGDIVTRHTRELKSVQLSGSVVAALIDEIPILAVLGTHLKDGLEVRD
                     ARELRNKECDRIEAIVKNMRSMGIRIDEFDDGFRIYPGSFRSSEIDSFDDHRIAMAFT
                     VAALIAEGGESIVHSAEAVRISFPEFYSTLNISLSSDRMAVHQ"
     gene            6560..7600
                     /locus_tag="PK937_12065"
     CDS             6560..7600
                     /locus_tag="PK937_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH33352.1"
                     /translation="MPKDKGKANTEKVKLGIVGAGAISQVIHLPILSKMNDVEIVAIA
                     DSDKTKVGALAEKYHIPNVFTDFEKLLAMDEIQGVHICTPNSMHAPMAKSALSAGKHV
                     LVEKPIALTYEESKTMVEESKKRKKILMVGMNHRFRPDAMVLKNFVKGKELGKIFYTK
                     SGWLRRRGSWNETDWAKKKNVSGGGVFMDLGIPMLDLSIWLMDNPKVHSVFSTMYSHF
                     QKDGVEDSAAVMIRFENDVVLSIEVSWTLLMENDFMYSNLFGTSGGALLNPLRIHKEM
                     HGNLVNVTPQKMEKPENNYKKSYEYELKHFVECIKNNRPSMSSGEEAMEIMRISEAIY
                     QSAKEKREIVLK"
     gene            7604..8512
                     /locus_tag="PK937_12070"
     CDS             7604..8512
                     /locus_tag="PK937_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNH33353.1"
                     /translation="MFGQRKIALALGGGGARGLAHIGVLKTLERHHIPMDIIVGSGIG
                     AVVGAMYAQTLHIAEVESRIKKFFLGDDFKKTGLHLFRRDMHPENFFGQIARAVGNEL
                     LIQLYADRMSLMKENRLGIVMDELLGDGLIENTRIQFAAVATNMITGKTEIFREGSIK
                     EAAKASSSIPGFLPPMQYEDKVLVDGAVAAQTPVDAAWEMGADYVIAVDTSRTLAGEA
                     PPVNMVDLLFRTHLITALQANALMCSRSTVHIRPEVGHHHWAAFEKMNELIAAGEKSV
                     DASINAILMALKKEKSRLSKHRLSKV"
     gene            complement(8524..9816)
                     /locus_tag="PK937_12075"
     CDS             complement(8524..9816)
                     /locus_tag="PK937_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNH33354.1"
                     /translation="MVIDILVILILVLLNGFFVAAEFAIVKVRSTQIDTIEQKNIRAR
                     IAKEVIMHLDAYLSATQLGITITSIALGWIGEPAISRMIEPIINYFGITNPQVIHALS
                     FGIGFSVITFLHITMGELGPKSIAIQYPKQTTLWIASPLHLFYVVFKPFIFILNKSAN
                     LMLRMVGIHPAGEHELSHSEEEIRMLIADGRKSGVIDATEYKLIENIFNFTETSVKEI
                     MIPRMDVFALDIEKPVVENLKSAIDSGYTRIPVYRESIDTLIGILYVKDLFKIDRDTI
                     TTASFETILRPAYFAPESISINRLMQDFLQQRFHMAIIIDEFGGTSGVVTLENIIEKI
                     VGQQIQDEYDDEKKEFEILSDGSYTVQAKMRIGDFNQQFQANIPEDDNYETLAGFLNN
                     VAGHIPNNGEEIRYQNLSFRITKKSPKLVQQVRFAKLT"
     gene            complement(9925..11433)
                     /locus_tag="PK937_12080"
     CDS             complement(9925..11433)
                     /locus_tag="PK937_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH33355.1"
                     /translation="MDKDLIAIQEARDIARAAKAAQKEFAKFTQQKVDTLVKAMADAG
                     LAAADRLARMAADETRMGRYEDKIVKNQFGTRDVYEYIKDMKTVGVLRYDDKLKFYEI
                     GTPMGVVCAIIPTTNPTSTVMYKILISLKAGNAIAIAPHPRAAKCTYEAMRILRDVAE
                     SMGAPKGLIGCIEHPTLEGTQALMKCPETSVILATGGPGIVREAYSSGKPALGVGSGN
                     VPAFIEKTANVKKAVADIVAGKCFDYGLLCSSENSIVADAALRDQVITELKANRAYVC
                     NNEEKIKLQQLMFPKGKLNTQIIGLPAPKIAQMAGVAAPSDATVLVVPCEKVGKEEPL
                     SAEKLSPVLSLFWVNGWEQGCDRALEILNFGGIGHTMSIHSQDNDVIMKFGLEKPAFR
                     ICVNTVSTLGAVGYTTGLAPSMTLGPGTLGGSITTDNIMPYHLMNVKRLAFEIRPFKS
                     SLVHGNTESQAKDTVKKTAEIPSAPVSSSSKNDSLSEKEIDKIVEEFIRSRK"
     gene            complement(11521..11679)
                     /locus_tag="PK937_12085"
     CDS             complement(11521..11679)
                     /locus_tag="PK937_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33356.1"
                     /translation="MLGDKLISKGVINQSQLDECLKEQQSSGKKLGEVIIAKGYATQA
                     QIDAALAG"
     gene            complement(11709..>12856)
                     /locus_tag="PK937_12090"
     CDS             complement(11709..>12856)
                     /locus_tag="PK937_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNH33357.1"
                     /translation="GIAYLFSNYDIEVRLKDINHQAITLGLDSAKKVYERALKRRKLT
                     KQTFSRKMSYITGALDYSGFRRCDLVVEAIVENMDVKKKVIGELSSHVSDSCIVASNT
                     SSLSITEMAKAYKHPDKFIGMHFFNPVHKMPLVEIIRGEKTSDATVAAIYALSKKLGK
                     QPIVVKDGPGFLVNRLLMPYLNEAGHLMEQGVTIELLDKTMLDFGMPMGPALLIDEVG
                     IDVAYKVGHIFYDAFGERVQPSRVVEKLHEAKRLGKKGKLGFYKHAGDKPQVDPEVYA
                     LIEVNQTSTIRSEEMVERMTYTMINEAAMCLAEGIVSQPSDVEIGMIFGTGFAPFRGG
                     LLRYADTEGLDKIVAQLEKYAHTYGVRFTPCDYLVQLQKSGKKFYNE"
BASE COUNT         3484 a         2677 c         3000 g         3695 t
ORIGIN      
        1 ggtacattat tgaccaaaaa catttccatt atcggttcca cgttgcgtaa caaatcgcat
       61 gaagaaaaat cagatctgac aaaaagtatg agagaactga tcatgccgca tttcacttcc
      121 ggtttatttc gtccgattat tgattcggaa tattcatggt ttgacgcaga ggccgctcat
      181 atgcgaatga agtctaatct taatatcgga aaaatcattc ttcgaatagg cgtgtgagtt
      241 tcgtattgaa atcgtaattg gcctatcgta agttcatatt attggaaacc tatgtgttat
      301 ttttgcgtat gccagcttag atagcatttt tgaaataaaa cgggttgcat ggatcctaag
      361 aacaaattgt ttcgtgtcgc attgattttt attccgattc tcttcgtgat cggggtgatt
      421 ctcaattcgt ttatgttcta tgcgaaaatt cgttttagtg cgggggactg gggcagtgaa
      481 cttctgagta ttgctattat tggtttggtt ttgtacgtag cgtttaatta ccctcggttt
      541 gaatcaatca gtatcacaca aaaattgcga ttatcgcttt ttttcgtcgt aacgatctat
      601 ggtgtgggcc tgttgatgac gctcgtcacc cagcccgtat ttttcacgcc gcgttactct
      661 ggctcctatg tactcagagc ttatacatgg tcaggtgtca tagtcgccca tgtgatcgga
      721 ttcgtggccg tattgtcgct gggttcatgc cttcggattt tgcagacatt gattttctat
      781 cgtcgaaaaa aaaatacggc tacttttttt catctgttct tactttttgg cgctttgacg
      841 gccttgtcaa ccgcgattac cggacgccca ctgaagtacg atccgtcctc taatcacatc
      901 atcacattta accttcttgt tattaccact atttttcttc tgatcaatgc gggacgtgtg
      961 acgtgggtaa ctgttctcaa ccgtcgtcag aagtttctta catttatagg agggttggta
     1021 tttaccctaa tttcggccgg acttttgggt gcggagattg gtggcggagc cacctatgct
     1081 gacatggtgt attcctatag ccgtacagcc gggagttttc tttttttaat gacggttttt
     1141 gtttttgtat atagtatcag tacgacgatc aatacactgc tgcacttgcc gacggccgct
     1201 atttatgata aaaaagttcg cgaaatcaat tcaatttatt cgctgagccg tacgatcaat
     1261 tcgctctttg atgtggaaaa aatcgtgatc tccgtgaccg aattggtgtg cgatgcgaca
     1321 ggcgccaatg cgtgctggct agaactggcc aacacaaaaa accctgcctt ccgtacccat
     1381 tttgattttg cggctgtacg tcaaagagaa ccgtttcagg ttcactttct cgaagttagt
     1441 tttcgtaaat tcctttctaa acatgaaaat gcaagattga taccatccaa cgatcagcat
     1501 caccattact atctgaccga accgacggat tggatcatca ataacaaaca gccgattctg
     1561 atcaatcaag tcaaacgcga taaacgaaca aaagatctga agcgcacacc catcgagtca
     1621 cttgtcggtg tgcctctgat cacgtcggac ggcatgctcg ggattatttt tgccgtcaaa
     1681 tcaacttcgt tcggatttga tcaggatgac attgcgatca tatcagcgtt agccaatcag
     1741 acgacggtag ctatcgaaaa cacgcgtttg gtcaaagagt cacttgaaaa agaacgtctt
     1801 gcggaagaat tgcgcatcgc ccatgatgtg cagatgaaac ttataccgca ggaaattccc
     1861 gatatacgcc atgacgtatc taaaatgacg cttgatatcg gcgcgacgac agtacctgcc
     1921 aatgaagtag ggggtgatta ttatgatttt gttcgtttgg ccgactggcg ttgcggaatt
     1981 gccgtagccg acgtatccgg aaaaggtacc tctgccgctt tttatatggc ggagatcaaa
     2041 ggcataattc aagcattagc cggtatttat tccgctccta aagacctttt gattgccgtc
     2101 aatgatgtgc ttttcggtac gattgatcgc aaatctttta ttacgttgct gtatgcggat
     2161 gtggatatat ctaagagcga actgcgtttt gcgcgggccg gccactgtcc tttactgcac
     2221 atgcgtgatg gtgtcaaaac ctatgttcag cccaatggca tcggcttagg attggataaa
     2281 ggtaatctct ttcgtcaggc tatcgaagaa aagacaatac gaattgacga tcgggatgtt
     2341 tttgtcatgt acagcgacgg attggtcgaa gcgcgtaatg cgaaggatga agagtacggt
     2401 gaagagcgcc tcagtaaatg tcttgacggt gtcgagcatc ttccggccgg ggttattaaa
     2461 gataaaatca tagcggatgt aaaacggttt gtcggctcgg cgaaaacaca cgatgatttg
     2521 acttgcgttg ttttccggtt ggccgattta cagaaacaag ttgttccggt ggcccctgtc
     2581 acagaagcgg aaaccgggga aattacggcc gtacgacgcg tttgatttgt gcttgaaata
     2641 gatgaaaaat tgaagtacaa tatttttcaa aaagcgacac ttcagttttc attttataac
     2701 gataggaata aaccatgaac gggttcgaag tcacgcgaaa agacgagcag gatatcactg
     2761 ttctgtactt gaagggatat cttgacgcgc acacgtatcc gcattttgaa ggcgagcttc
     2821 aaaaattgat ggaagaaaaa cgttaccgga tcgtcgtgga ttttcaggag ctgagttata
     2881 tttcaagcgc cgggttaggc gtttttatgg gctttattga aacggttcgc gaaaatcaag
     2941 gtgatatcaa actgtgtacg atgtccacca aagtatataa agtttttgat ttgctcggtt
     3001 ttccgacgat ttatcagata ctcaaagatc cttcggaagc gcgcgatcac tttgtacagg
     3061 ccaataaata ataatcgtgg aaacaacggt gtccagcgtt acaacaaatt catttaatat
     3121 ggccatcccg agccgtacgg acaatctagg gatcattcgt gagtttatca cgtcgatcgc
     3181 ccgacggttt ggatttaatg atgatgcgat cggcgaaatc gaactggccg ttgatgaagc
     3241 atgtgccaat gtgatcaagc acgcgtataa atatgacgaa tccaaaaata ttgatattac
     3301 ggtagaaacc aacggtagta aatttaccat cattatctcc gatgaaggcg aagggtttga
     3361 tccggatcgt ctggaaacgc ctcaacagcg tcttcagaaa catgcgcgcg gaggccttgg
     3421 gatagcgctg atcaaacgaa tgatggatga agttgttttc gatattcatc ccggtgaaaa
     3481 aaatcgtgtt aaaatgatta aatacgtttc gtgacggctt cgacactaat tcatgacgtt
     3541 cacttcttta actcttgaat ccctgcaaac cacgtcctct ctgattcttc cgtttttttt
     3601 attatatcca gaaattcatt accgcttccg attctttccg ttcagccgat atttcaaaaa
     3661 agaacctgaa atcattgcgg atattccaca tcgtatagag cgtggtcgaa ggatgccggt
     3721 ctttgtttta gtcaaagatg cgcatctcta tcccacgtcc attctctcag ttcgtaccat
     3781 cattacaaaa ggcgcaaata gtgatgaatt gattttccag gtacaagagg aaatacgcca
     3841 acattggtgg tggaaaaagt gggatatcgc cgtaaacgaa tcatggatag gccataccgt
     3901 tcaactgcgt attgttgtcg aatacagaac agccggggtc acgcataccg tggaagccga
     3961 caatataatt ggaacaacaa aggcgccgtt tgatgtctat gtgagcgggg atcattggcc
     4021 gcaaccccaa gggtggttta acggtgattt gcatgtacac agcgactata ccgaagatgc
     4081 tatagaatat ggagctccgc tgcccatgct cagagctgcg agcgaggcat tgggattaaa
     4141 ttttatcgcc gtgacagacc attcttatga cttggataat aaaaaagagg attataatca
     4201 atccgaccct acgcttgcgc aatggcgtac atttatcgaa tccgtgaaac agatgaatga
     4261 agaatcctct tcaaaagttt tattactggc cggtgaagag ctatcatgta tgaatattgc
     4321 cgatgaaaat atacatgcgt tgattcttaa ctcggatcat tttttttatg gttccggcga
     4381 cggaggtaaa aatccactaa acacatcctc cgaacatacg gtatcgggaa tggcccgttt
     4441 attgaataaa gaagctctta ttatggctgc gcatccttta gaaaaaccat ctccaatgga
     4501 gcgtgctttt ttacaacggg ggatatggcg tttagaagat ttttataatc gttcggcgga
     4561 ggtcttacaa attttaaatg gtagtaacga tacggcattc aagcaaggtt tgggcttgtg
     4621 ggtgcatctt ttgttgtcag gccgacgggt ttatattgtt gccggaaacg atgcgcatgg
     4681 caactttaat cgtaaccgtc aggtgcgtat tcctttttgg accatcgaag atatatcgca
     4741 ccacaaacaa ctgggttggg cgcgaaccgt attgtacctc ggtgaaaaaa aacctaatac
     4801 gcaaaatacg atcgatgcca ttcgtgaagg atgtgcgatt atttcgacgg gtccatacgt
     4861 tgtttttaat gttttaaacg aggcaggttt ttctagccgc atcggacaaa acgctaaagg
     4921 ccgacggttt cgcgttgcat gggaagcgtc gagcacacaa gagtttggtg cgctcgaaac
     4981 gattagcgtc tttcaaggag tcatcggcga atcgcacgaa aaggtctcta cgatgacggt
     5041 tgaatcatgg tgcgatcaag gagagtttca cacgacggca gacgcgccga gttatatacg
     5101 tttgagcgtt actacgaaaa gtcaaaattt ctgctacact aacccgattt ggcttgaata
     5161 aagccccttt accgttgctt tttcatcgtt gcatgtgtat ttttgttatc aattatttgc
     5221 atgaaatgtt atggcaacgg cgctcattaa acctgcgaca aagatcagaa ttgattgtag
     5281 aatacccggt gataagtcta tcacacaccg tgcagttatt ttttcagcca tgtctgaaag
     5341 catcgtttcc ttgtttaata tagcaaccgg cgctgatgtg caaagtacta tagattgtat
     5401 gcgccgattg ggcgtgaagg ttgatcaaaa cggttcagag ttgcacataa ccggatgcgg
     5461 tatgcatgga tttgtggcac catccgcgcc tttgtattgc gggaactcag gaacaacggc
     5521 aagactattg gcgggtattc tttccggtca aagttttaaa tctgaactta gtggtgacgc
     5581 gtctttatca cgacgcccga tgagacgcat tattgaaccg ctgactcaaa tgggtgccaa
     5641 aataaaaaca tcggcgcatc aaacattacc gatagaaatc gaaggtacag aattaaaagc
     5701 cattgattac agtttaccga ttgcaagcgc tcaagtcaaa agttgtattt tgctggccgg
     5761 tcattatgct aaaggaataa ctaccgttcg cgaatcggtc atgacacggg atcacacaga
     5821 gcgccttcta ccaacgataa aaaaatcggt tgaagatggc cggattagtt tgtcggttac
     5881 gggaggtgaa tcttttcgtt ttgatcatat gacgattcca ggcgatttat cttcggcagc
     5941 atttttaatt gcgacggggc tcttgctgcc tgaaagcgaa gtgactatac gccatgtttc
     6001 actaaaccct actcgcattg cctttttgga aattttgaaa aaaatgggcg cggatattac
     6061 tattcataat atcacagagg cgcgagaacc ttatggtgat atcgtcacgc gccacacccg
     6121 cgaattaaaa tcagtacaat taagcggctc ggttgtagct gctttgattg atgaaattcc
     6181 gatattggcc gtgctaggaa cccatttgaa agatggtttg gaagtccgtg atgcacgtga
     6241 gctacgcaat aaagaatgtg atcgtatcga agccattgta aaaaacatgc ggtctatggg
     6301 aatacgaatt gatgaatttg atgacggatt tcgtatttat cccggttcat ttcgatcgtc
     6361 agaaatagat tcatttgatg atcatcgcat tgcgatggca tttacagttg ccgctttgat
     6421 tgctgaaggc ggcgaatcca tcgtacattc tgcagaggct gtgcgtattt ctttccctga
     6481 attttatagt acattaaata tatccttatc ttcggaccgc atggcggttc atcaatagtt
     6541 ttattcttac ggaggtttta tgcccaagga caaaggcaaa gcgaatacgg aaaaagtgaa
     6601 attgggaatc gtcggtgccg gcgctatttc acaggtaatc catttaccta tactttcgaa
     6661 aatgaacgat gttgaaatcg tggctatcgc ggattcggat aaaaccaaag taggagcgct
     6721 ggcggagaag tatcatatcc ccaatgtttt tactgatttt gaaaaacttt tagcaatgga
     6781 tgaaattcag ggcgttcata tatgtacgcc taacagtatg catgcgccga tggctaaatc
     6841 agcattatcc gcagggaaac atgtactggt ggaaaagccc atcgcgctta cctatgagga
     6901 atccaagacc atggtcgaag aatccaaaaa acggaagaag attcttatgg tgggaatgaa
     6961 tcatcgtttc cgcccggatg cgatggtact taaaaatttc gttaaaggta aagaactcgg
     7021 taaaatattt tacaccaaat cgggttggct gcgtcgtcgc ggcagctgga atgaaaccga
     7081 ttgggccaaa aagaaaaatg tttccggcgg cggtgttttt atggatctgg gaattccgat
     7141 gcttgatctt tccatatggc tgatggacaa tcctaaagtt catagcgttt ttagcacgat
     7201 gtacagccat tttcagaaag acggcgtcga agattcggcg gcggtaatga ttcgttttga
     7261 aaacgatgtc gtgctttcca tcgaagtcag ctggacatta cttatggaaa atgattttat
     7321 gtattccaat ctttttggaa caagcggggg cgctttgctc aatccgctac gtattcacaa
     7381 agaaatgcac ggcaatcttg tcaatgtgac gcctcaaaaa atggaaaagc cggaaaataa
     7441 ttataaaaaa tcttatgaat acgaactgaa acactttgtg gaatgcatta aaaataaccg
     7501 gccttcgatg tccagcggcg aagaagccat ggagatcatg cgcatttcgg aagcgattta
     7561 tcaatcggcg aaagaaaaac gcgaaatcgt tttaaaataa cgaatgtttg gacaacggaa
     7621 aattgcatta gctttgggcg gcggcggcgc acgcggtttg gcccacatcg gtgtactcaa
     7681 aactttagaa cgccatcata ttccgatgga tatcatcgtc ggtagcggta taggcgctgt
     7741 tgtcggtgcg atgtatgctc agactttgca tattgctgag gtggagtctc ggataaaaaa
     7801 attctttctc ggcgatgatt ttaaaaaaac gggactgcat ctttttcgcc gcgatatgca
     7861 tcctgaaaat tttttcggac aaatagcccg ggcggtaggg aatgagttgc tgattcagct
     7921 atacgccgat cggatgtctt tgatgaaaga aaaccgactc ggcatcgtga tggatgaatt
     7981 gttgggagat ggtttaatcg aaaatacccg cattcaattt gccgctgttg caacaaacat
     8041 gatcaccgga aaaaccgaaa ttttcagaga aggttcgatc aaagaagccg cgaaggccag
     8101 ttcatctatc ccgggttttt taccgccgat gcaatacgaa gataaagtct tagtggatgg
     8161 cgctgtcgct gcgcagacgc cggtagatgc ggcatgggag atgggggctg actatgtgat
     8221 cgctgttgat acgtcgcgta ctctggccgg agaggcaccg ccggtgaata tggttgattt
     8281 attatttaga actcatctta tcacagcact gcaagccaac gctcttatgt gttccaggtc
     8341 aacggtacat attcgacctg aagtcgggca tcatcattgg gcggcatttg agaaaatgaa
     8401 cgaactcata gcggccggtg aaaagtctgt ggatgcctct ataaatgcga tattaatggc
     8461 actcaaaaaa gaaaaaagcc ggttatctaa acaccggctt tcgaaagttt gaagcatagc
     8521 cgattaagta agtttggcaa aacgcacttg ttgaacgagc ttcggtgatt tttttgtaat
     8581 cctgaaactt agattttgat accgaatttc ttctccgtta ttgggaatat ggcccgccac
     8641 attattcaga aaaccggcta acgtttcgta gttatcgtcc tcgggtatgt tggcctgaaa
     8701 ttgctgatta aaatcgccga ttcgcatctt ggcctgtacc gtataactgc cgtcagataa
     8761 aatttcaaat tccttttttt catcgtcgta ttcgtcctga atctgctgac cgactatttt
     8821 ttcgattatg ttttcaagcg ttacgacacc gctcgttcca ccgaattcat caatgataat
     8881 cgccatatga aatcgctgtt gaagaaagtc ctgcatcaga cgattgatac tgatgctttc
     8941 cggtgcaaaa taagcagggc gtagtatcgt ttcgaaggaa gcggtcgtta tcgtatcgcg
     9001 gtcaattttg aataagtctt tgacataaag aataccgatc aatgtatcaa ttgactcacg
     9061 gtacaccggt atgcgcgtat agccgctgtc tatcgccgat ttcagatttt caacaaccgg
     9121 tttctctata tccaaggcaa atacatccat gcggggaatc attatttctt ttaccgaagt
     9181 ttcggtaaaa ttgaaaatgt tttcgattaa cttgtactcc gtagcatcaa tcacaccgct
     9241 tttacgacca tcagcaatga gcatacgaat ttcttcttcg gagtgagata actcatgttc
     9301 accggctgga tgaatcccga ccatacgaag catgagatta gcgcttttat tgagtatgaa
     9361 tatgaatggt ttgaaaacga cataaaaaag atggagcggc gatgcaatcc aaagcgttgt
     9421 ttgtttagga tattggatag caatggattt ggggcccagt tcgcccatcg taatatgtaa
     9481 aaaagtaatc acggaaaaac cgataccaaa agacaaggca tgaatcactt gcggattggt
     9541 tattccaaaa taattgataa taggttcaat catgcgtgaa atagcgggtt cgccgatcca
     9601 gccgagcgca atacttgtaa tcgtaatacc aagttgggtt gcggacagat acgcatcaag
     9661 gtgcataata acttctttag cgatgcgtgc gcggatgttt ttttgttcga tggtgtcgat
     9721 ttgcgttgaa cgcactttga cgatagcaaa ctcggcggcg acaaaaaaac cgttgagtaa
     9781 aacgagtata aggattacta agatatcaat aaccacgagg tgtgctatct cctttaatga
     9841 aaaagcactt cacgttgtgc gaagtgctta aaagctggtg caatgtcacg taaaacacgt
     9901 taatttgcaa tagaatttta taggctattt tctgctgcga atgaattcct ctacgatttt
     9961 atcaatttct ttttccgaca aagaatcgtt cttagacgaa gaagaaacgg gggcagacgg
    10021 tatttcggcg gtttttttga ctgtatcttt tgcctgtgat tcggtattgc cgtgaactaa
    10081 agaagatttg aacgggcgta tttcaaaggc aaggcgtttc acgttcatca aatgatacgg
    10141 catgatatta tctgtcgtaa tggagcctcc gagtgtgccg ggtccgagcg tcatagacgg
    10201 cgcgagtccg gtcgtatatc cgacagcgcc taaggtggaa acggtattca cgcaaatacg
    10261 gaaggccggt ttttcgagac cgaacttcat gatcacatca ttatcctgag agtgaatgga
    10321 catcgtatgt cctatacctc cgaaattgag aatttccagt gcgcggtcac agccttgttc
    10381 ccacccgttt acccaaaaaa gtgataatac cggtgaaagt ttttcggccg acaggggttc
    10441 ttctttgccg actttttcgc aagggacgac cagtactgtc gcatccgatg gtgccgctac
    10501 accggccatt tgcgcaattt taggtgcagg aaggccgata atctgggtat tcaattttcc
    10561 tttaggaaac atcagttgct gaagcttaat tttttcttcg ttattgcaaa catatgcgcg
    10621 attggctttc aattcagtaa taacctgatc acgcagtgca gcgtcggcaa cgatggagtt
    10681 ttcagatgaa cataacaaac cgtaatcgaa acatttaccc gcgacgatat cggcgactgc
    10741 ttttttaaca ttagccgttt tttcgataaa agcgggaaca ttgccggagc ccacgcccag
    10801 agcaggttta cctgaactgt atgcttcgcg tacaataccg ggcccgccgg tagccaaaat
    10861 caccgaagtc tccgggcact tcatcaatgc ttgtgttcct tcaagtgtcg gatgttcgat
    10921 gcaaccgatg aggcctttgg gcgcacccat agattccgca acgtcgcgga ggatgcgcat
    10981 ggcttcgtag gtacacttgg ccgcacgcgg atgaggagcg atagcgatgg cattaccggc
    11041 tttaagcgat attaatattt tgtacatcac ggtggatgtg ggattggtcg tagggattat
    11101 agcgcataca acacccatcg gggtgccgat ctcataaaat ttgagtttgt catcgtagcg
    11161 caagacacca accgttttca tgtccttgat gtattcgtac acatcgcgtg tgccaaattg
    11221 attttttaca atcttatcct cataacgtcc catacgcgtt tcgtcggcgg ccatacgcgc
    11281 caaacgatct gcggcggcta gcccggcatc ggccatcgct ttgacaaggg tatctacttt
    11341 ttgttgcgta aattttgcaa actctttttg tgcagctttg gcggctcggg cgatgtcgcg
    11401 tgcttcctga atagcgatga ggtctttatc cataagggct gggcctgaat ataaaatgaa
    11461 atgttaaatg cattcgaata tacgcggctg tgcaaaagat ttcaagcgat aatccgaagt
    11521 ctatccggct aaagcggcat caatctgagc ttgcgtcgca tatcctttgg cgatgataac
    11581 ttcaccgagt tttttaccgc tgctttgttg ttctttgagg cattcgtcca attgggattg
    11641 attgataaca cctttgctga tgagtttatc gccaagcatg attcgatcct ttcgtttgtt
    11701 tgttttagtt attcgttata aaatttctta cctgattttt gtaactgtac caaataatcg
    11761 catggggtaa aacgtacgcc gtaagtgtgc gcatattttt caagctgtgc aactatttta
    11821 tcaaggcctt cagtgtcggc gtatcgcaaa aggccaccgc gaaatggggc aaaacctgtt
    11881 ccgaagatca ttccgatttc gacatcgctt ggttggctga caattccttc ggccaaacac
    11941 atggccgctt cgttgatcat cgtataagtc atgcgttcga ccatttcttc cgaacggata
    12001 gtcgaagttt ggtttacttc tataagcgca tagacttcag gatcaacctg aggtttgtcg
    12061 cctgcgtgtt tataaaatcc taattttcct ttttttccga ggcgtttggc ttcatgaagt
    12121 ttttcgacaa cgcgggaagg ttgtacgcgt tcgccgaatg catcataaaa aatatggccg
    12181 actttgtacg ctacgtcaat tccgacttca tcgataagta gcgccgggcc catcggcata
    12241 ccaaaatcga gcatggtttt atccaatagt tcgatggtaa cgccttgctc catcagatgc
    12301 ccggcttcat tgagataggg catcaagaga cgattgacga ggaatcccgg tccgtccttc
    12361 acgacgatag gttgtttgcc gagttttttt gacaacgcat agatcgcggc aacagttgca
    12421 tccgatgttt tttctccgcg aataatttca acgagcggca ttttatgtac cgggttaaaa
    12481 aaatgcattc caataaattt gtcgggatgt ttataagcct tcgccatttc ggttatggat
    12541 aaggaagacg tattggacgc tacgatacag ctatcgctga cgtgacttga aagttctccg
    12601 ataacttttt tctttacatc catattttcg acaatggctt cgacaaccag atcgcaacgg
    12661 cgaaaaccgg aataatccaa tgcgccggtg atataggaca ttttcctgct gaacgtttgt
    12721 ttggttagtt tacgccgttt caacgcccgc tcatatacct ttttagccga gtcgagaccc
    12781 aatgtgatcg cttgatggtt gatgtctttt agtcgcactt cgatatcata gttagaaaaa
    12841 agataggcga ttccgc
//
LOCUS       DAOQGF010000096        12675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2301_length_12675_cov_7.236846, whole genome shotgun sequence.
ACCESSION   DAOQGF010000096 DAOQGF010000000
VERSION     DAOQGF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_12675_cov_7.236846"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK937_12095"
     CDS             complement(<1..350)
                     /locus_tag="PK937_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33358.1"
                     /translation="MIRKLAVSLGIVMMISCDTSHHGRDRCEEGMLSASDAVWTHSEG
                     DLQNTKRATGMRIKGCQAGPITKPVVQWAFELGGAGTAAAPVIGDDGTIYINGEYPGE
                     PLGGGWRNAGLMAI"
     gene            complement(714..3530)
                     /locus_tag="PK937_12100"
     CDS             complement(714..3530)
                     /locus_tag="PK937_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH33359.1"
                     /translation="MQFTVYSQSAASLSGIVRDSDTREPMIGAYLKLAGTSLGAVSDR
                     EGRFRISGIASGSYTLKVSYLGYTTYEKKFTVAAGDHTKMEILLKAGSIELSGSEVYG
                     MREGQAKALNLQKNADNQQVVTSSEIIKLFPDPNISEALQRMPGVAVLRDQGEGRYVF
                     LRGLEPKYSSVKINGQPVPSPEGDIRQVSLDVISSSMVSRIEVSKTLTPDMDADAVAG
                     AINLVTKNALDFDQQMWQVSAGTGRNMLEGKPLANFDITYGNRFGEEKRLGVFVNAMG
                     YYTHRNSDDMERVWGERAFNGQNHYFMNEFELRDYNAIRKRLSFNAAADYRFSETSDI
                     HFGAVHNYFRDDEIRRRLRIKLSEGTAPVRARRFKFVSDSSVTMDSAVVERETKDWAL
                     TRSLTSFQMSGHAQWADMDWTYSAALSRATEAIPDRFDFTTRQTDVNLKYDMTNRQFP
                     KFAFLSGDPYDPALFKLKNFEVQEYDITEWDRIAELNIRKSLRILSNGDYVQSGAKVR
                     SKAKERNDEHNRATIANPNAPPFMNFFAESDEDINFLYSRYRFGTSADPSLIRRWADT
                     ANLINSANYFADESFITNYDADEMIIAVYGMTKLNVNRVDVVAGVRMEKTYIDYNGVK
                     VVQNGKAIRSTQLGYPDTLHFAGYPIDTVSLKGHYEYYQFFPSLNLKYNITDRFSVRA
                     AIGRAIFRADYFDLIPYEIFDYQNFTISRGNTYLRPTVSWNYDISADYYLKDVGIIAG
                     GFYYKVLNDFIYRRQYIDTTLNEGSPWGVNQWRVNQKQNGKVARLFGAEFNWQQTFTF
                     LPPNFDGLGIYLNYAYTHSQAYYPQRPGNKNTLPGQPNHVGNFALTYEKKGFAARVAW
                     NYSGPYVLTVSDSVDTDFDTYVDRHLQLDISASQKLTKHIRVYLELNNLTNEPYREYV
                     GSRKRLRQQEYYSWWGKLGAEYTF"
     gene            complement(3690..4817)
                     /locus_tag="PK937_12105"
     CDS             complement(3690..4817)
                     /locus_tag="PK937_12105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH33360.1"
                     /translation="MKIAYLSTFYPFRGGIAQFNASLYRTFEKQHTVRAFTFTRQYPD
                     LLFPGKSQYVTPDDHADPIPSRALLDTVNPLSYRRTALELAAFAPDMLVMKFWMPFFA
                     PSLGYVAGYLRRRGTKVITVLDNVIPHEKRPGDMALIRYFLRRNSGFVAMSKTVEQDL
                     LALMPDARYTLKAHPLYDHYGPAIGRDEACAKLGIPKNKKTILFFGFIRDYKGLDLLI
                     RSVARLSESHHLIIAGEVYGSFDKYQALINELGLQNRISLFTRYIDDAEVPLFFSAAQ
                     CCVLPYRSATQSGIVQIAYHYHLPVLVTDVGGLSEMVSGGQTGAIIPKAEPESIATAI
                     SDYFDKGYYEAMSQAIAGRRAEYSWHGFAAAIEKLYAQVSG"
     gene            complement(4814..5911)
                     /locus_tag="PK937_12110"
     CDS             complement(4814..5911)
                     /locus_tag="PK937_12110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNH33361.1"
                     /translation="MKYSEIKFDCRFFRGDIPCKPNKLHGKICNTCDQYQPISRRILI
                     IKLGAIGDVIRTTPLVVRYHKMFPNCHVSWITHTPEVVPSSIVDKVYKFDFKSVYALS
                     HQHFDIAINLDKDIEACALLNDVHAKEKFGFGLKDGHAAAENPLAEHKFITGAFDSIS
                     RANTKNYLEEIFEICGLTFEREPYILDVDNKYLSVADPVLRAAQGKKIIGLNTGCGDR
                     WLTRLWPEAYWVELIQILQMNGFFPLLLGGPAEDAMNKNYAKMTGAYYPGTFTLQEFI
                     ALSSRCDVVLTAVSMMMHIAIATRVPLVLFNNIFNPHEFELYDNGIMVQPATGCDCYF
                     GNTCSRTHHCMLDIPVDSVYQAIVRLKDRKA"
     gene            complement(5928..7547)
                     /gene="rfaE1"
                     /locus_tag="PK937_12115"
     CDS             complement(5928..7547)
                     /gene="rfaE1"
                     /locus_tag="PK937_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNH33362.1"
                     /translation="MNLATLRQKLLDSSRPKILVVGDIILDKFIWGSVDRISPEAPVQ
                     VVNMRRETLALGGAANVAHNLAAIACDVTMLGVIGQDAHGAALKEALGQAGIHSSHVV
                     TDTTRPTTTKTRVMAGSQHVVRIDNEVADAVSPDVENQIIASVKKIIGNIDAVIISDY
                     QKGVLTDKVLLNTIQLARQAGVPSIVDPKRRDFSAYSGATIIKPNLKEAEAATGKKLT
                     NADEVLKAGRSLVNQYQTDAVLITRGKDGMMLVEARTHTSIAATAREVFDVTGAGDTV
                     SAFLGMMLASKATLAEAAEVANLAAGVAVSRMGTVTVTREEVLHQMEQSASGSRKILT
                     AGEASVLIAGIRGKKKIVFTNGCFDILHVGHITLLNQARALGDALVVGLNSDASVRRL
                     KGEKRPIVSEQERAHILAALASVDYVVLFDEDTPLELIRQIQPDILVKGSDYTLDRVV
                     GRAEVESYGGRVALVDLVVGFSTTNIVESILQAYGVKQENKTTDDKNLAHAENTMQRI
                     TSLQDRYEEKIDRIENKNKSDALNALHPKKK"
     gene            complement(7551..8513)
                     /locus_tag="PK937_12120"
     CDS             complement(7551..8513)
                     /locus_tag="PK937_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HNH33363.1"
                     /translation="MIQIQDILNAYKTVQPIVHKTPLMQSHALDEKTGNAVFFKCENF
                     QKVGAFKIRGAYNKIASLSAEQKAKGVVAFSSGNHAQGVALASRLLGTTSKIVMPDNS
                     PKSKMEATRAYGAEVIVYDFLKEDREAIGKRISESEGRTLVPPFNDEYIMAGQGTVSV
                     ELVEQISDLDYAFIPCGGGGLLSGNAVALKHFYPHIKVVGVETDTANDAYQSFNKGEI
                     VKIAPPPTIADGMRTLALGDKTFPVIRRYVDAMLTVSDHAVIEAMYWIYSRLKIVVEP
                     TAAVPFAAVMQNTLQLRGKKIAVILSGGNIDLDDFFELLKRQAA"
     gene            8666..9910
                     /gene="hutI"
                     /locus_tag="PK937_12125"
     CDS             8666..9910
                     /gene="hutI"
                     /locus_tag="PK937_12125"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNH33364.1"
                     /translation="MQKNTPMTLFIQNIKEVVFFEKDLFRTANAVDIRIENGMIADIT
                     PTGTTSAPAHATILDGASYVALPGLIDSHTHTIFGGSREDEFEMKLRGATYQDIAAKG
                     GGIKSTMHKTRAASKEELLQLGIRRCQSALALGITTMEMKSGYGLSVEAEIKILETIR
                     DVRQHTPIEITSTFLGAHTIPPEYSQQREAYIRLVCETMIPMVTEQRLAEFCDAFCET
                     NVFTPDETRHIFETAKKYGMKLKLHADQLTNTGGAELCAEMGAISADHLETTSDKGLA
                     ALAGSNVVAGLLPGCSFYLRMQYPPARKMIDMGIPVALATDFNPGSCPTQNLQLIMSI
                     ACTQMRMTPDEAIKGVTLHAARALDKTHIGNIAVGMQADMVLFDVPGYRYIPYNFGQN
                     HVAHVIKKGRVVHSASSLGENR"
     gene            complement(9887..11617)
                     /locus_tag="PK937_12130"
     CDS             complement(9887..11617)
                     /locus_tag="PK937_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33365.1"
                     /translation="MLLIGGLGTYAIIRLSDESSLIIKDNYKSLGYAQNMIGALDDAY
                     ASNDAAVMMDTLALMKAYARFDSNFSHELNNITEPGERELVMQLYGSFNEFKSNPKNT
                     RERYNCYLRMRTSLRAVFDVNMNAILRKHDYAQASARTIMIAMAAVGIPCLILTLIFV
                     LRFPGYIAAPIRTLSEGIRSIAEKNYERRVNITTKDEFGEVAASFNRMASRLEEYDNS
                     NWARILFEKTRIETIIRNMHDPILVLDERGFVLFTNPAFSEVVAVPEKNLVGQYASTA
                     ADQNIHLHSILNPAMNDSTDDTIRLRSPNKDLIFRREQFSVIRTDDDTHTSQALGRVI
                     ILKNVTQFHELDMAKTNFMATISHELKTPLSSIDLSVKLLQDGRTGMLSDEQKKLVRA
                     IRQENSRLMKLVSELLDMTQLESGQLRLTIGRVSVMEIILLATDALEFQLHQKSIRIT
                     TDIPHDVPFVSADLDKTVWVMVNLLTNAVRYTAEGGTIRIAVRKNDQTVEFSVEDYGE
                     GIDPKHHARIFERYIRLENPSTGDGGGTGLGLSTARDFILAQRGTIQMESVPGKGSRF
                     YFTLPVFAEG"
     gene            complement(11741..>12675)
                     /locus_tag="PK937_12135"
     CDS             complement(11741..>12675)
                     /locus_tag="PK937_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNH33366.1"
                     /translation="PRLKIVHTSASVHGQIYMPAINWLLCAGCVALVLTFKSSGALAS
                     AYGITVTATMTITTMGFFLVTTRIRRWSIFWVAPLCAGLFAIDLSFFVSNAIKFLDGG
                     FVPVTLGFVICGVMLLWSWGKKKLSHVYAPDGMTVADLMALKDVEWKPVIPRRIHFFS
                     PSPVTSESSLVPLTLQIFVSRIHVLPRELSFVTVKIHNQPYVEGPRVEHYTLRNDVRA
                     VVIHYGYMEDPDIVTILERHEIKGNIVVGDHEIFSEHEHGLHYLQVRWFRLLLRLSLP
                     SYRYFGVQRMSRIMKEVIPVEIGKKGAVLLDVDY"
BASE COUNT         3453 a         3175 c         2754 g         3293 t
ORIGIN      
        1 ttgatggcca tcagtccggc attgcgccag ccgcccccca gcggctctcc ggggtattcc
       61 ccgttgatat agatcgtacc gtcatcgccg atcaccggcg cggcggccgt ccccgcgccg
      121 cccaattcaa acgcccactg tacaaccggc ttcgtgatcg ggcccgcctg gcatcctttg
      181 atgcgcatac ccgttgcacg tttcgtattc tgcaaatcgc cctccgaatg cgtccatacc
      241 gcatccgatg cggacaacat accctcttca caacgatcac gaccgtgatg cgatgtatca
      301 caggaaatca tcatcactat ccctagcgat acagccagct ttcgtatcat gatggtattc
      361 tttttatttg ttatgcgcat ggtgtgattc tttgggtttt gtcttcatct gtatatgata
      421 gtaccgtaat catcaaaatt atcacaattt tttttggggt tgctcagatt tatgcagata
      481 aagacaaaat cactaagtaa taacgaatcg ctacccgaaa gttttaaaaa acactttaac
      541 ccttgggacg acagataaga tcacctaaat gcaaaatatt aagcccctta gaggcagaat
      601 tgatgattct gtgcgtcaaa tgaacagcac tgtagaaaat tgggtttatc aaagcacacg
      661 ataaagtagc tcaacccaac gtcgtcaggc tgatctccgt ctagagatag aaattaaaat
      721 gtatattccg cacccaattt accccaccac gaataatatt cttgctgacg taaccgctta
      781 cgggaaccga cgtactcacg atacggttca ttggtcaggt tgttgagctc aaggtaaaca
      841 cgaatatgtt tggtcaattt ctgggaagcg gaaatatcca actgcaaatg ccgatccaca
      901 tacgtatcaa aatccgtatc taccgaatcg gaaaccgtta acacataagg cccgctataa
      961 ttccacgcta cgcgtgcggc aaatcctttt ttctcgtaag tcaaagcaaa attgcccaca
     1021 tgattaggct gacccggcaa cgtattttta ttacccggcc tttgcggata gtaggcttgc
     1081 gaatgcgtat aggcatagtt caaataaatg ccaagcccgt caaaattggg aggtaaaaaa
     1141 gtaaatgttt gctgccaatt aaattcggca ccaaaaagtc gggcgacttt tccattttgt
     1201 ttttgattaa ctcgccactg attgacaccc cacggtgacc cctcattaag ggtagtgtct
     1261 atatattgac gccgatagat aaaatcattt aatactttat agtaaaagcc gccggctatg
     1321 ataccaacat cttttagata ataatcggcg gatatatcgt aattccaaga caccgtagga
     1381 cgtaaataag tattcccccg cgaaatcgta aaattttggt aatcaaaaat ttcgtaaggt
     1441 atcaaatcaa agtaatccgc cctgaatatg gcacgaccga tcgccgctcg aactgaaaaa
     1501 cggtcggtta tattgtactt cagattaaga gaagggaaaa actgatagta ttcataatgt
     1561 ccttttaacg atacggtatc aataggatat ccggcgaagt gcaatgtatc cggatagcct
     1621 aattgcgtgc ttcgaatagc ttttccgttt tgcactactt tgactccgtt ataatcaata
     1681 tatgtttttt ccatacgcac accagccaca acatccactc gattaacatt caatttagtc
     1741 attccataaa cggctatgat catttcgtca gcatcataat tggtaataaa actctcatcc
     1801 gcaaaatagt ttgcgctgtt aatcaagttg gccgtatcag cccaacgccg aatgagcgaa
     1861 ggatccgctg acgtgccgaa tcggtatcgc gaataaagaa aatttatgtc ttcatccgat
     1921 tccgcaaaaa aattcatgaa tggcggagca ttgggattcg ctatcgttgc acgattgtgt
     1981 tcatcattgc gttcttttgc cttggacctc acttttgcac cggactggac ataatcgccg
     2041 ttcgataata ttctcaaaga cttgcgaata ttcagctcag caatacgatc ccattccgta
     2101 atgtcgtatt cttgcacttc aaagttctta agtttgaaaa gggccgggtc ataaggatcg
     2161 ccggaaagaa acgcgaactt tggaaattga cggttcgtca tgtcgtattt aagattgaca
     2221 tccgtctgac gggtggtgaa atcaaagcga tccggaatcg cttctgttgc gcgcgataaa
     2281 gcggcactgt atgtccagtc catatctgcc cactgtgcgt gaccactcat ctgaaaactc
     2341 gtaaggcttc gggtcaacgc ccagtcttta gtttctcgct ctactacggc cgaatccatg
     2401 gttaccgatg aatccgacac aaatttaaaa cgtcgcgcac gaacaggagc ggtaccttcg
     2461 ctcagtttaa ttcgcaaacg acgacgtatc tcgtcatctc tgaaataatt atgtacggcc
     2521 ccgaagtgta tgtcgctcgt ttctgaaaaa cgataatctg ccgcggcatt gaagcttaac
     2581 cttttccgta ttgcattata gtctctgagt tcaaattcat tcatgaagta gtgattctgg
     2641 ccattgaaag cgcgttcacc ccaaacgcgt tccatgtcat cggaattgcg atgcgtgtaa
     2701 taacccatcg cattgacaaa cacgcctaat cttttttctt ccccgaaacg gttgccgtag
     2761 gttatatcaa aattggccag aggtttgcct tcaagcatat tacgacctgt cccggcggac
     2821 acttgccaca tttgttgatc aaaatccaac gcattttttg taaccagatt gatcgcaccg
     2881 gcgacggcat cggcatccat gtccggcgtg agcgttttgg aaacttcaat acgggatacc
     2941 atgctcgaag aaattacatc caaactaacc tggcgaatat cgccctccgg cgaaggcacc
     3001 ggttgaccgt taattttgac agacgaatat ttaggttcta atccgcgtaa aaaaacataa
     3061 cgcccttcgc cttgatcgcg cagtacggct acgcccggca tgcgttgcaa agcctcggaa
     3121 atattggggt ccggaaaaag tttgataatc tccgacgaag taacaacttg ctgattgtct
     3181 gcattttttt gaagatttaa tgctttcgcc tgaccttcgc gcataccata cacttcactc
     3241 ccggacaatt cgatactgcc ggccttaagc aaaatttcca tcttagtatg atcaccggca
     3301 gctacggtaa attttttttc gtaagttgta tagccgagat atgaaacttt gagtgtgtat
     3361 gaccccgatg caataccgct gatacgaaaa cgtccttccc tatcggatac agcacccaaa
     3421 gaagtgcctg ccaatttcaa atacgcgccg atcatcggtt cgcgcgtatc gctatcccgc
     3481 acaataccac taagggaagc cgccgactgc gaataaaccg tgaactgcat cagaaatagg
     3541 aaaatccaaa gaaaagtgaa gttctttgaa aaagaataca tatagatcga accgattaga
     3601 ttaaggggta tattctaaat cttaatttca gaacggagtg aaaagtaacc tatataattt
     3661 gatttttcaa tttctatttt aagttcacgc tatccggaaa cctgcgcata tagcttttct
     3721 atcgcggctg caaatccgtg ccatgaatac tccgcacgcc gacccgctat cgcttgtgac
     3781 atagcttcat aatatccctt gtcaaaatag tctgaaattg ctgttgcgat ggattccggc
     3841 tccgcctttg gaatgatcgc acccgtctga ccgccggata ccatttccga taaaccaccg
     3901 acatcggtaa ctaatacggg caggtgataa tgatacgcga tttgcacaat gccgctctga
     3961 gttgcggaac ggtaaggcaa tacgcagcac tgtgccgcgg aaaaaaacaa cggcacttca
     4021 gcatcgtcaa tataacgagt gaatagtgaa atgcgatttt gtaaccctaa ttcattgatc
     4081 aaagcctgat atttatcgaa gcttccatac acttcgccgg ctatgatcag atgatgcgat
     4141 tcactcaatc gcgcaacgga acggatcaaa agatcaagtc ctttgtagtc gcggataaaa
     4201 ccaaaaaaaa gaatagtttt tttgtttttc ggaataccca gcttcgcaca cgcttcatca
     4261 cggccgatag ccggaccgta atgatcgtac agcgggtgcg ctttgagcgt gtatcgtgcg
     4321 tcaggcatca atgccaataa atcctgttcg accgttttgc tcatcgcgac gaagccgcta
     4381 ttgcgacgga gaaaataacg aatcaacgcc atgtcccccg gtcgcttttc gtgcgggata
     4441 acattatcaa gcacagtgat cactttggtg ccgcgtcggc gaagataacc cgccacataa
     4501 cccaacgatg gtgcaaaaaa tggcatccaa aacttcatca ccagcatatc cggagcaaac
     4561 gcggctaatt ctaatgccgt gcgacgatac gagagcggat tgacggtatc caatagcgca
     4621 cgggacggta tcggatcggc gtgatcatcg ggagttacgt attggctttt gccgggaaaa
     4681 agaagatcgg gatattgacg cgtaaaggta aaagcccgaa cagtgtgttg tttttcaaac
     4741 gtgcggtaca gtgatgcatt aaactgagca atcccgccgc ggaagggata aaacgtggac
     4801 agataagcaa ttttcatgct ttgcggtctt tcagtcgaac aatcgcctga tataccgaat
     4861 ctacaggaat atcaagcatg cagtgatgcg tgcgggaaca ggtattgccg aaataacaat
     4921 cgcacccggt agccggctga accatgatac cgttatcgta caattcgaat tcatgcggat
     4981 taaaaatatt attaaaaagt accagcggta cccgcgttgc gatcgcaatg tgcatcatca
     5041 tggaaaccgc tgtcagcact acatcacagc gagaagacaa cgcgataaat tcctgcaatg
     5101 taaatgttcc cggataatac gcaccggtca ttttagcata gtttttgttc atcgcatctt
     5161 ctgcaggccc gcccagaagc agaggaaaaa aaccattcat ctgaagaatc tggatcaatt
     5221 cgacccaata cgcctccggc cacaatcgcg tgagccatcg gtctccacaa ccggtattga
     5281 gtccgatgat ttttttgccc tgcgcggcac gtaaaaccgg atcggctaca gaaagatatt
     5341 tgttatccac atccaaaata tagggctcac gttcaaacgt aagcccgcat atttcaaaaa
     5401 tctcttcgag gtaattttta gtattggcgc gcgagatgct gtcaaaagcg cccgtaataa
     5461 acttatgttc tgccagcgga ttttctgctg ccgcatgccc gtctttcagg ccaaagccga
     5521 atttttcttt ggcatgcaca tcgttgagca aagcacaggc ttcgatatct ttatctaagt
     5581 tgattgcgat gtcaaagtgt tgatgactta acgcatacac ggatttgaaa tcaaatttgt
     5641 aaactttgtc cacgatcgaa gagggtacga cctcgggtgt gtgcgtgatc caagatacat
     5701 ggcaattggg gaacatttta tgataacgca ccacaagcgg cgtcgtacga atcacatcac
     5761 cgatagcgcc cagcttgatg ataagtatgc ggcgcgaaat cggttgatac tgatcgcagg
     5821 tattgcaaat tttgccgtgc aatttattag gtttacacgg aatatcaccg cggaaaaagc
     5881 ggcaatcaaa tttgatttcg gagtacttca tagattagaa gatctgatta tttctttttt
     5941 gggtgcaatg cgtttagcgc gtcggatttg tttttatttt caatgcgatc aattttttct
     6001 tcataccgat cctgcaacga tgtgatgcgc tgcatggtat tttcagcatg ggccaaattt
     6061 ttatcgtcgg ttgttttatt ttcttgtttt acgccgtaag cctgcaaaat agattcgaca
     6121 atattggtcg tactaaaccc gacgaccaga tcaacaagcg cgacccgtcc gccatacgac
     6181 tccacttccg cacgtccgac aacgcgatcc aaggtgtagt ccgaaccttt gactaaaata
     6241 tccggctgaa tctgtcgtat cagttccaac ggcgtatctt catcaaacaa cacgacgtaa
     6301 tctaccgatg ccagcgccgc aagaatatgg gcgcgttcct gctccgaaac gatcgggcgt
     6361 ttttcgccct taagtcgccg cacggaagca tcgctattaa gtcctacgac cagcgcatca
     6421 ccgagtgccc gtgcctgatt gagcaacgtg atatgaccga catggagaat atcaaaacaa
     6481 ccgttggtaa atacgatttt cttttttccg cgtataccgg cgatgagtac actcgcttca
     6541 ccggccgtca ggatttttcg tgaaccgctt gcggactgct ccatctgatg cagcacttct
     6601 tcccgcgtca ccgtcaccgt gcccatacga ctcaccgcca caccggcagc cagattggcc
     6661 acttcggccg cttccgcaag cgtagctttt gaggccaaca tcattcccaa aaaagcgctc
     6721 accgtatcac cggcacccgt aacatcaaaa acttcacgcg ccgtagccgc aatcgaggta
     6781 tgcgtgcgcg cttcgacgag catcatgccg tccttgccgc gtgtgatcag caccgcatcg
     6841 gtttgatatt gattgaccaa ggaccgccct gcttttaaaa cttcgtccgc attggtcaat
     6901 ttttttcccg tggccgcttc agcttctttg agattaggct tgatgatcgt agcgcccgaa
     6961 tacgccgaaa aatcacggcg tttaggatcc acgatactcg gaacaccggc ttgacgggcc
     7021 aattgtatcg tattgagtaa gactttatcg gtcaaaacgc ctttttgata atcagaaata
     7081 atcactgcat caatgttacc gataattttt tttacggaag cgatgatttg attttcaacg
     7141 tcgggcgaaa cggcatccgc gacttcgtta tcaatacgaa cgacgtgctg gcttccggcc
     7201 ataacgcggg ttttggtggt cgtagggcgc gtcgtatcgg taaccacgtg actggaatga
     7261 ataccggcct gcccaagcgc ttctttcagc gccgcgccgt gcgcatcctg tccgatcaca
     7321 cccaacatcg taacatcaca agcaatcgcc gcaagattgt gggcgacatt tgctgcgcca
     7381 cccagcgcca gagtctctct gcgcatattg acgacctgca ccggcgcttc gggcgaaatg
     7441 cgatcaacgc tgccccaaat aaatttgtct aaaataatat cgccgaccac cagtatctta
     7501 gggcgagagg aatcaagtaa tttttgccgg agcgttgcta agttcatgat ctaagcggcc
     7561 tgtcgtttga gaagttcaaa aaaatcatcc aagtctatat ttccgccgga aagtatcacg
     7621 gcgatttttt ttccgcgcaa ttgcaatgta ttttgcatga ccgcagcaaa aggtactgca
     7681 gcggtcggct cgacaacaat cttgagtcgt gaataaatcc aatacatcgc ttcaatcacg
     7741 gcgtgatccg acacagtgag catcgcatcc acataacgcc ggatcacagg aaaggttttg
     7801 tcgccgagag cgagggttcg catgccgtcc gcgatcgtcg gcggcggcgc tatttttaca
     7861 atttcgcctt tgttaaacga ttggtatgca tcattagcgg tgtctgtctc tacgccgacc
     7921 actttgatgt gcggataaaa atgtttgagg gcgacggcat tacccgataa aagcccaccg
     7981 ccaccgcaag gaataaaggc atagtccaaa tccgatattt gctccaccaa ttcaacgctc
     8041 accgtaccct gaccggccat gatgtattca tcgttaaacg gcggcaccaa cgtacgtccc
     8101 tctgattcgg aaatccgttt accgatcgct tcacgatctt ccttgagaaa atcgtaaaca
     8161 atcacctcag caccatacgc gcgggtcgct tccattttag atttcggcga attgtccggc
     8221 atgacaattt tggacgtcgt accaagcaag cgcgacgcca gggccacgcc ttgcgcatga
     8281 ttaccagagg aaaaagctac tacgcctttg gctttttgtt ccgctgaaag cgatgcgatt
     8341 ttattatacg ccccgcgtat tttgaaagcg cccacttttt gaaaattttc gcacttaaaa
     8401 aaaaccgcgt tccccgtttt ttcatccagc gcatgggact gcatcagcgg agttttatgt
     8461 acaatcggct gaaccgtttt ataggcattg agtatatctt ggatttgtat cataagccga
     8521 aaatgatatt gaaattgacg cttaaaatca accttgtttc atgctgtttc aaagccttag
     8581 acagcctacc gtcctctgtt tttttggtta tacatcactg tttcttttgc ggtgtgtttt
     8641 tattaccatt gcacgattat ttcacttgca aaaaaataca cccatgacgc tttttatcca
     8701 aaacatcaaa gaagtcgttt tttttgaaaa agatttgttc cgcacagcca acgcggtaga
     8761 catacgtatc gaaaacggaa tgatcgcaga cattactccc accgggacca cttcagcgcc
     8821 ggcccatgca acgattttag acggtgcatc gtatgtcgcg cttccgggat tgattgattc
     8881 ccacacccat acgattttcg gcggttcccg cgaagatgaa tttgaaatga aactgcgcgg
     8941 cgccacctat caagacatcg cggctaaagg cggcggcatc aaaagtacaa tgcacaaaac
     9001 ccgcgcggca tccaaagagg aattgctgca actcggtatc cgacgttgtc agtcggcatt
     9061 ggcactcggc atcaccacga tggaaatgaa aagcggttac ggtttgtcgg tcgaagcgga
     9121 aatcaaaata ctcgaaacca ttcgcgatgt gcggcagcat acaccaattg aaattacctc
     9181 cactttcctc ggcgcgcaca ccattcctcc ggagtacagc cagcaacgcg aagcatatat
     9241 tcgtttggtt tgcgagacca tgattcccat ggtaacggaa caacgactgg ctgaattttg
     9301 tgatgcgttt tgcgagacca acgtatttac accggatgaa acgcgtcaca tttttgaaac
     9361 ggccaaaaaa tacgggatga agttaaagtt gcatgcggat caattgacca ataccggagg
     9421 tgccgaatta tgcgcggaga tgggcgctat ttccgccgat catcttgaaa ccacttccga
     9481 taaagggctt gcggcactgg ccggatccaa tgttgtcgcc ggtttattgc ccggctgttc
     9541 attttacctg cgtatgcaat atccgccggc tcgtaaaatg atcgacatgg gcatcccggt
     9601 cgctttagct acggatttta atccgggttc ttgtccgacg caaaatctac agctcatcat
     9661 gtccatcgcc tgcacccaaa tgcgcatgac gccggacgaa gcgatcaaag gcgtgacgct
     9721 gcatgccgcg cgtgccctgg ataaaacaca tatcggaaat atcgccgtag gtatgcaagc
     9781 ggatatggtt ttgtttgatg taccgggata taggtacatc ccttataatt tcggacaaaa
     9841 tcatgtcgcg cacgtcatca aaaaaggccg tgtggtacac tccgcatcat ccctcggcga
     9901 aaacaggtaa tgtaaagtaa aaacgactgc ctttgccggg tacactttcc atttgaatcg
     9961 tgccgcgctg tgccaaaata aaatctctgg ccgtggataa acccaaaccc gttcctccgc
    10021 catcgccggt cgaaggattt tctaaacgta tgtaccgttc aaatatgcgg gcatgatgtt
    10081 tgggatcaat gccttcgcca taatcttcca cactaaattc aacggtctga tcattttttc
    10141 tgacagcgat acggattgtc ccgccctccg cggtgtatcg tacggcattg gtcagaagat
    10201 tgaccatgac ccatactgtt ttatccagat ccgcgcttac aaacggtacg tcatgaggaa
    10261 tatcggtcgt tatgcgaata gacttttgat gcaactgaaa ttctaaggcg tccgttgcca
    10321 ataatataat ttccatgacc gacacacgcc cgatagtcaa tcgaagttgc ccgctttcca
    10381 attgggtcat atccagcagc tcgctcacta atttcatcag gcgcgaattt tcctgacgaa
    10441 tcgcgcgaac gagtttcttt tgttcgtcac tcagcatgcc ggtccgccca tcctgtaata
    10501 atttgacgct cagatcaatg gaagacaaag gcgttttcaa ttcgtgcgaa atcgtcgcca
    10561 taaaattggt tttggccata tccagttcat gaaactgcgt gacatttttt aaaataataa
    10621 ctcgccccaa tgcctggctg gtgtgcgtgt cgtcatccgt ccgaatcaca gagaattgct
    10681 cacgccggaa aataagatct ttattcggac ttcgtagcct gatcgtgtca tcggtcgaat
    10741 cgttcatcgc cggattgagt attgaatgca aatgaatatt ttgatcggcg gccgtcgaag
    10801 catactggcc gaccagattt ttttccggca cggcgacaac ttctgaaaaa gccggattgg
    10861 tgaaaagtac aaatcctctc tcatccagca ccaatatcgg atcatgcata ttgcgaatga
    10921 tcgtttcgat gcgcgttttt tcaaaaagta tacgtgccca gttactgtta tcatactctt
    10981 ccaaacgtga cgccatacgg ttaaacgaag cggccacttc gccgaattca tccttggtcg
    11041 ttatgttaac tcggcgttca taattttttt cagcaatact gcgtatacct tcggacaacg
    11101 tacgtatcgg cgccgcgata taaccgggaa aacgcaaaac aaaaatcaac gtcagaatca
    11161 ggcaaggtat accaaccgcg gccatcgcta tcatgatcgt tcgcgccgac gcttgggcat
    11221 aatcatgctt cctgagtatg gcattcatat taacgtcaaa aacagcacgc agactagtcc
    11281 gcatacggag atagcaatta taacgttctc gggtattttt ggggttcgat ttaaattcgt
    11341 taaacgatcc gtataattgc atcaccaact cgcgttcacc gggctcggtg atattgttga
    11401 gttcgtgcga aaaattactg tcaaaccgcg catacgcttt catcagagcc agcgtatcca
    11461 tcataaccgc cgcatcgttc gacgcataag cgtcatccaa tgcacctatc atgttttgtg
    11521 cataacccag tgatttatag ttatctttta tgatcaacga agattcatcc gaaagcctga
    11581 taatcgcata cgtacccaat cctccgatga gtaacatcac gctccaaagc aaaagtaatc
    11641 cagcaaataa tttttgtttc aacgtcaaca atggacatcc actttcatag tatgttgtta
    11701 aaagtacgcc gccggacttt cgtaaaacag tgaaaatata tcaataatcc acatccagaa
    11761 gtacggcccc tttttttccg atttcgacgg gaatcacttc cttcatgatc cgtgacattc
    11821 tttgaacccc gaaataacgg taggagggta atgatagccg cagcagcaag cggaaccatc
    11881 gcacttgtaa ataatgcaat ccgtgttcat gttctgaaaa tatttcatga tcgccgacga
    11941 ctatattacc tttgatctca tgccgttcca ggatcgttac gatatccgga tcttccatat
    12001 aaccgtagtg tataaccacg gcacgcacgt cattccgcaa cgtatagtgt tcaacacgag
    12061 ggccctccac gtacggttga ttatgaattt ttacggtcac aaacgaaagt tcgcgcggta
    12121 atacgtggat gcgtgaaaca aaaatttgaa gcgtaagcgg gacaaggctg ctttccgatg
    12181 tgaccggtga gggcgagaaa aaatgaatcc ggcgcggaat gaccggtttc cattcgacat
    12241 ctttgagtgc catcagatcg gcgacggtca tgccatccgg cgcatacaca tggctgagtt
    12301 tcttcttacc ccacgaccag agcaacatta cgccacatat cacaaaacct aacgtaacgg
    12361 gaacaaatcc gccgtcgaga aatttgatcg cattggatac gaagaaactg aggtctatcg
    12421 caaataatcc cgcacacagc ggcgccaccc aaaatataga ccaccggcga atgcgcgtag
    12481 ttacgagaaa aaaacccata gtcgtgatcg tcatggtggc cgtcaccgtg ataccgtaag
    12541 ccgaagccaa tgcgccggag cttttgaatg tcaacaccag cgccacacat cctgcacaca
    12601 aaagccaatt gatcgccggc atatatatct gaccatgcac ggaagcgctg gtgtgaacta
    12661 tttttaaccg cggaa
//
LOCUS       DAOQGF010000097        12568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2330_length_12568_cov_6.475505, whole genome shotgun sequence.
ACCESSION   DAOQGF010000097 DAOQGF010000000
VERSION     DAOQGF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2330_length_12568_cov_6.475505"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PK937_12140"
     CDS             <1..231
                     /locus_tag="PK937_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33367.1"
                     /translation="REGHEQFPIAEGYFKKAVQLNNSPYAWLYLGKIYDYQKRRNEAI
                     EALRTVMKLLKPDDRYREEARKKLREYFPDVE"
     gene            221..1048
                     /gene="folP"
                     /locus_tag="PK937_12145"
     CDS             221..1048
                     /gene="folP"
                     /locus_tag="PK937_12145"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNH33368.1"
                     /translation="MSNNSEALYSALKTRVHVMGILNVTPDSFSDGGRFFSTEKAVVH
                     AEQLIADGADILDIGGESTRPGAKPLTLKDELDRVLPVLRAIRKKSDIPISIDTYKTD
                     VAKAALDEGADLINDISGMTMDEGIAGVAAQNDVPLVIMHIRGTPSQMQNNPSYDDVI
                     NEVKQFLETQCTKAQQMGACKLIIDPGFGFGKRYEDNFTLLKNLRTFTALGYPLMAGT
                     SRKSFLGKAFDAPPANRLEGTLISNIYAVQQGATLLRVHDVSSMKRALHIHQMIETA"
     gene            1050..1880
                     /gene="cdaA"
                     /locus_tag="PK937_12150"
     CDS             1050..1880
                     /gene="cdaA"
                     /locus_tag="PK937_12150"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNH33369.1"
                     /translation="MQDELFKIWTISVTAIDLLDIAAVTIIFYRAYLVIQGTTASRML
                     MGLIFILILSIAVQALNMSGMIWLVDQIRTVWVIAFVIIFQPELRRLLLFFGQSGFLR
                     NIIKLGIPEYIEEIIKACELLAKRRNGAIIAIERSTGLRNIVETGLHLEANISQQLLL
                     SIFNPRSPLHDGAVIIENDQIAAAKCLLPLSPNPYLETSVGTRHRAGVGLSEQSDAIV
                     IIISEETGKISVAFKGVLHRNLTPSELRGFIEEVITSGKTRGLWRRIRRQPRKTLVNN
                     "
     gene            1956..7115
                     /locus_tag="PK937_12155"
     CDS             1956..7115
                     /locus_tag="PK937_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNH33370.1"
                     /translation="MNKNFVLVISLLCSTNFLTAQTTDMVSPRGYATVIKQEPGELIY
                     EFSIPSPDIDSVIGDDKALHSVALKHAESYLQADGYAVPSLNYWIELPVQANPTVRAT
                     AMNKKTLHGIRLSSARDDWSADSQADPTSGLRSSRNSSTSALASIRYHGKMRDIPLYK
                     LNITPIQYNAQQHTLEITTRMQVVVRYNANTRTPVQEINNGDIDALLSDRLINAEPAR
                     RVRQLQTAASSTSIKLSKSSSSSSATAAITGGPYIRMTVDKEGIYKISVRELQDLSGV
                     NLQSVDPRTLRLIGQGLEERVYVRSKKTDRLAADDIIEFYGEPYRAKHNKYLRDIPSK
                     QGHYLDPWATDNVYFLIWGSSAGLRLIEEEGSLQERDPLSVIKPASYTTTKHFEEDNV
                     LYDVKDINLIRPVAEEDIWVYDNGISYSAAVGSSTTSREYEFELPGVPTGTIQQFSLR
                     INLQAISTQKHFVNVELNNNLITPNPITWTDATKLQQDIPFSSDLLSPTGTNTLRIST
                     TNSTERNSDIFAMNWFEITYKRNYSAADNILIFTPDVPISGTTLHEFTLSGFNDLNIS
                     IYKRSSSRIVNPVIRNTSIQGKTTYQALFQDDVPISGIEYIAVGETAKLTPKTVTAYN
                     PGNLQTGNHDARYLIITSSALRDSALRLENYRKGQGYSAETITIEDIYDEFSYGAPGP
                     YAIRDFLRYVYSAANWKGSQGNPLYVCLIGDASRQPKTSSNEIVPTQFIQTQKYGAAS
                     SDYWFCLADDQDIIPDFFIGRIPVINATQLNAYIDKIIAYEKGPSGNWKNKIVFIGGS
                     EEQRATLGNPEIPRDVFRYQSTLLINNRLAQRFTPERIYAYPGQEYGGGAGAVTDFFS
                     DGALIITYLGHGGGGVWGDRDIAGEPMMNHDRINLITPNGGRYPIVLSMTCFVGAFDS
                     DGQFALGETMMLSRDRGAAAVLAASGTGWIIGDYQMLDNMMNEFLAQNTTAGQAIAKG
                     KTNYLIFRGEQDFSAEGANRSSTTFQSLVPQSMVYQFNFLGDPALKIRAPRARTMALS
                     NYSPSRSASFNVSGATDFSSGSGTAEIFQTKPTTDSVSGGANKSSFANVVSIPFTITD
                     GQYDFSINLNSIPNSALRSGLTGIRLYGESSDGQKYFNSHTDFYIDGTFIADMKTIPA
                     APTSSDIIRFSATVSDPQGVARVIAHYTVTGPIASSGVDTLTLTSGNTYESQGIGPFA
                     ENDKITYVLTIYDTNGDSTQSPSPNFKILSGIDLNIPTPLTKSIYVGGSSEVRINAVV
                     QNLGFAKAENVNVRFYEGNPLSTGVLIGETFVQVEGSITNSGVISSDTASILSNLSNG
                     EHVIAVWVDPDSGINDVSRSNNLRFQTIRLGAFNVTPSLGTTFNGTKNDTVSVDSALR
                     ISIPPGVYTQNGTMSLNVTGEYTIVNQPDISPAIPYGASAAQVYEPGFSRLIADGQPI
                     HLIMRYDTLRYNATYTDSLSVYRWDSGNRKWTILSASQTKSRGQISAILTSNEDRAPW
                     MLMINRDRTAPVIETTIEGQLFTQGGIAPRQPRVSAIVYDRNGVSLDRNHYAITVDNA
                     PISASQIVLPDSVPNSNTVTFTLNLNQAFDDGQHEVLFTAKDVNGNTSKTDTLNFQVI
                     RKFDIESYGNYPNPFINTTTFAFRVEAPEPLEALDISIYTVSGRRIRKITDADAIASG
                     KNITAVGYHEVTWDALDEDGRPIANGVYFYRIRGKLNGKTIEKKGKLAYFR"
     gene            7125..8174
                     /locus_tag="PK937_12160"
     CDS             7125..8174
                     /locus_tag="PK937_12160"
                     /inference="COORDINATES: protein motif:HMM:NF015634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HNH33371.1"
                     /translation="MKKSVRTLILLFISAATVITAQNRRYANAFLEIPAGVRAASMGS
                     AYSAIANDATAFFWNPAGSVLVNRHEVTGSYSSQFGGLAQYHVLGYNHQINNRYSFAV
                     GWIRYSISNIPENADLSGSASEHAVPGFDFAALQHGHFSYADNAFFFTFARMNTLRLN
                     LGWAYSDFPIQIPVGASFKIIRGGTSGISGENGVVLNDVRKSGIGVDIGTMIMFGMND
                     LLEIPTLGDFVMSFNLQDATTTAVQYNKISSDVRAQDIVKANFKFGMSYIQGLDEWKS
                     NILLSYESNTRYANDRHYGLEWEYNKLAALRLGIDNGTLTYGAGFQYNHIHIDYALNN
                     HALGYTHRIGTSYKF"
     gene            8191..9084
                     /locus_tag="PK937_12165"
     CDS             8191..9084
                     /locus_tag="PK937_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33372.1"
                     /translation="MFRNSILSLTFIGLATMIFTGCPDEVNPPEPRPSKVRIKAHNGD
                     TLAVDRGTRPIEGALQLLRLEWSPVTDSDNLITEYIVYRSGGLDSAFAEIAVRTVNEA
                     ETDSFIEDAVIAEKTYYYYVVAKDSKGNTTDISDYLDEENLYTYVMPFRLAQKASPRR
                     PYNQDTVVTTKPQFSWCIFGDEPGYYVIKVATPSGQLVWLAYQQPGTFGENCGAQESM
                     TLNKQTYSYIDEFFDGIPGDSLYATDSPTRVVYANPNFFEGARLQKGVYQWRVDSYFG
                     KNNASRSPWVSFTVNRDYKNP"
     gene            9150..10127
                     /gene="pta"
                     /locus_tag="PK937_12170"
     CDS             9150..10127
                     /gene="pta"
                     /locus_tag="PK937_12170"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNH33373.1"
                     /translation="MSIIASIRERARHKKCRIVLPEGEEDRTLLAAKIIINEQIAKPI
                     LLGDADKIRNRARELQVDLPSSVSIIDPKRSEKTADYASELFNLRKAKGMTYEQAKQH
                     MHSVLYYGAMMVRRSECEGFVGGAVSTTADVLRAAIHCVGTAPDINTVSSIFLMVLPD
                     GRAITYGDCAVMPYPDAAQLADIAIASAESHKQLTGEEPMVAMLSFSTKGSAKHEAVD
                     KVVQAHSIVREKRPDIKIDGELQFDAAFIESIGQRKAPGSAVAGKANVFIFPNLDAGN
                     ICYKVTERIGKAQAIGPVIQGLAKPINDLSRGCTPEDIANVTAICALKA"
     gene            complement(10197..12182)
                     /gene="dxs"
                     /locus_tag="PK937_12175"
     CDS             complement(10197..12182)
                     /gene="dxs"
                     /locus_tag="PK937_12175"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNH33374.1"
                     /translation="MSKIFDRVNLPKDIKSLSSDELVQLAGELRAYVIDEISRIGGHL
                     APSLGVVEISVALHHVFDTPTDKVIWDVGHQAYVHKLLTGRKDQFHTIRQTDGISGFC
                     KIHESEYDAFGAGHASTSISAAVGMAIARDQLKKNNKVVAVIGDGSLTGGMAFEAMNN
                     AGSLKTDMIVVLNDNMMSISSNVGALSNYLTDLISAPLYNKVKTEVWNALGHLHEVGD
                     RVRHGLSKMDESIKSMLVPGHFFESLGFRYFGPIDGHDLPRLIKVLRDVKDLKGPILI
                     HTLTKKGKGMPLEEQDVEKYKVNANKFHAVSPPKKVNASETVAAPTYTEVFGKTVVKL
                     CKMHSHAVGITAAMADGTGLKFLAKEMPERFFDVGIAEQHAVTMAAAMSLNGVKPIVA
                     IYSTFLQRAYDQIVHDVAIQKIPVLFCLDRAGLVGADGPTHHGALDLSYLRCIQGMVI
                     MAPKDENELKDMVYTGLMYDKGPIAVRFPRGNGIGVTVTETFEKIPIGQSEFVQRGKD
                     IALLAVGNMVQHALSAAQELLRNGYSATVVNIRFVKPLDEAMLFEICRDFDDVITIED
                     NVTTGGFGSGVLETIYSSDFIERGQKQDKDFVQKMARLRLKRLGLPDAIVEHGDNDVL
                     YERVGLDPASIARDAKTMIEKRRGGIQVETIMAAAAR"
BASE COUNT         3388 a         3085 c         2787 g         3308 t
ORIGIN      
        1 cgcgaagggc atgaacaatt ccccattgcc gaagggtatt tcaaaaaagc cgtgcaactc
       61 aataattcgc cgtatgcttg gctgtatctc ggtaagatct atgattatca aaaacgccgc
      121 aacgaagcca tcgaagcttt acgaacggta atgaaattgc tcaaaccgga tgaccgttat
      181 cgcgaagaag cgcgtaaaaa actccgcgag tattttcccg atgtcgaata attcggaagc
      241 cctatacagc gctctaaaaa cgcgcgtcca tgtcatgggt attctcaatg tgactcccga
      301 ttctttttca gacggcggtc gttttttttc aacagaaaaa gccgtcgttc atgccgaaca
      361 acttatcgct gatggcgctg atatactgga tatcggcgga gagtccacgc gtcccggtgc
      421 gaaacctttg acactgaaag acgaacttga tcgcgttctt cccgtgcttc gcgcaattcg
      481 caaaaaatcc gacatcccta tttctattga cacctacaaa accgacgtgg cgaaggccgc
      541 attggacgaa ggcgcagacc tgatcaacga tatcagcggg atgacgatgg acgaaggtat
      601 cgccggggtg gccgctcaaa atgatgtccc tttggtcatt atgcacattc gcggcacacc
      661 gagtcagatg caaaataatc cgtcgtatga cgatgtaatc aacgaagtaa aacagtttct
      721 tgaaacccaa tgtaccaaag cccaacaaat gggcgcctgc aaattgatca ttgatccggg
      781 tttcggattt ggtaagcggt atgaagacaa ttttacatta ctcaaaaatt tacgtacttt
      841 tacggcgctg ggttatcctt tgatggcggg cacatcgcgc aaatcatttt taggaaaagc
      901 atttgatgcg ccgccggcga atcggttgga aggtacgctc atcagcaata tctatgctgt
      961 acagcaaggt gcaaccctat tgcgcgtaca tgatgtatcg tctatgaaac gtgcgctcca
     1021 tattcatcag atgattgaaa cggcctgaca tgcaagacga acttttcaaa atatggacga
     1081 tttccgtaac ggccatcgat ttgctggaca ttgcggccgt gacgattatt ttttatcgag
     1141 cctatttggt gattcaggga accacggcct cacgtatgct tatgggtttg atatttattt
     1201 tgattttatc cattgccgtg caagcgctca atatgagcgg aatgatctgg ctggtggatc
     1261 agattcgtac ggtttgggtt atcgcctttg tgattatttt ccaaccggag ctccggcgct
     1321 tacttctttt tttcggccaa agcggttttt tgcgaaatat catcaaactt ggaatccccg
     1381 aatacatcga agagatcatc aaagcttgcg aattgctcgc caaacggcgc aatggcgcca
     1441 tcattgccat tgagcgctcc acgggtttgc gcaatattgt agaaaccgga cttcatcttg
     1501 aggccaatat ctctcaacaa cttttgcttt cgatttttaa tccccgttca ccgctgcatg
     1561 acggcgccgt catcatcgaa aacgatcaga tcgccgcggc caaatgttta ctgccgctga
     1621 gccctaatcc ttaccttgaa acttcggtcg gtacacgtca ccgtgcggga gtcgggctgt
     1681 cggaacaatc ggacgcgatc gtgattatta tttcggaaga aaccgggaaa atatccgtcg
     1741 cattcaaagg cgtactccat cgcaatctga caccgtcgga actgcgcggt tttatcgaag
     1801 aagtgatcac ctccggaaaa acgcgcggcc tatggcgtcg tatccggcgt caaccccgaa
     1861 aaacgcttgt aaataattaa ccgaaatctc tgttttaccg tacaatatac atattaaccg
     1921 acactatgga ttttgttttt gcgaatacgc accgaatgaa taaaaatttt gttttagtaa
     1981 ttagtctgtt gtgcagcaca aattttttaa ccgcacaaac gacggacatg gtttcccctc
     2041 gcggttacgc caccgtcata aaacaagagc cgggcgaatt aatctatgaa ttctctattc
     2101 catcaccgga tattgactcg gttatcggcg atgataaagc cttgcactcg gtcgcactta
     2161 aacacgctga atcgtattta caagcggatg gctatgccgt accctcgttg aattattgga
     2221 ttgaactgcc tgtgcaagcc aatcccacgg ttcgcgctac agcaatgaac aaaaaaacac
     2281 tgcacggtat tcgcttgtct tctgcgcgcg atgattggtc ggccgattcg caagccgatc
     2341 ccacgtccgg actccggtcg tctcgcaatt cttctacttc agctttggca tccatccgtt
     2401 atcacggtaa aatgcgcgac attccgctgt ataaattgaa catcacgcca attcagtaca
     2461 atgcccaaca gcacactttg gaaataacga cccggatgca agtggtagtt cgttataacg
     2521 ccaacactcg tacaccggtt caggaaataa ataacgggga cattgacgct ttgctgagcg
     2581 accggctgat caatgccgaa ccggcacggc gcgtacgcca acttcagacg gccgcatctt
     2641 ccacgtcaat taaactttct aagtcttctt cttcttcctc cgcaacggca gcgattacag
     2701 gcggcccata catccgcatg accgtggaca aagaaggcat ttataaaata tccgtccgag
     2761 agcttcagga cttatccggc gttaacctcc aatccgtaga tccgcgcaca ttgcgtttga
     2821 tcggccaggg tttggaagaa cgcgtatacg ttcgttccaa gaaaacggat cgccttgcag
     2881 ccgatgatat tatcgaattt tacggtgaac cttaccgcgc aaaacacaat aagtatctgc
     2941 gtgatattcc gtccaaacaa ggccattatc ttgatccatg ggctaccgat aatgtttatt
     3001 ttttgatttg gggaagttct gccgggctgc gcctcatcga agaagaaggt tctcttcaag
     3061 agcgcgatcc attgtctgtg atcaaaccgg cttcttatac gacgacaaaa catttcgaag
     3121 aagataatgt actctacgat gtcaaagata ttaacctgat acgccccgta gcggaagaag
     3181 atatttgggt atatgacaac ggcatatcgt attctgcagc ggtaggttct tctacgacat
     3241 cgcgcgagta cgaatttgaa ttgcccggcg tccctacggg aacgattcag caatttagcc
     3301 tgcgcatcaa tctacaagcc atttctacac aaaaacattt tgtaaatgtc gaattgaata
     3361 ataatctgat cacacccaac cccatcacgt ggaccgacgc taccaaactt cagcaagata
     3421 ttcctttttc atcagaccta ttatcaccga caggtacaaa taccctccgc atctcaacga
     3481 cgaacagcac cgaaagaaat agcgatatct tcgctatgaa ctggtttgaa atcacatata
     3541 aaagaaacta ttccgcggct gacaatatac tgattttcac gccggatgta cccatttccg
     3601 gtacgacatt acacgaattc acactcagcg gttttaacga tctcaatatc agtatttaca
     3661 aacgcagttc aagccgcatc gttaaccctg ttattcgtaa tacgtccatt cagggtaaaa
     3721 ctacatatca ggcattattt caagatgatg taccgatatc cggtatcgaa tatatcgcgg
     3781 tcggtgaaac agccaaacta acgcctaaaa ccgtgacagc ctataatccc ggtaacttac
     3841 agaccggcaa tcatgatgcg cgatatctta tcattaccag tagtgcacta cgtgatagcg
     3901 cactgcgact ggagaactac cgcaagggcc aaggctattc agcggagaca attactatcg
     3961 aagatattta tgatgaattc agctatggcg cccccggacc gtatgccatt cgcgattttc
     4021 tgaggtatgt ctattccgct gcgaactgga aaggctcaca aggtaacccc ctatacgtat
     4081 gcttgatcgg cgatgcatcc cggcagccta aaacaagttc aaacgaaatc gttccgacgc
     4141 aatttataca aacacagaag tacggtgcgg catccagcga ttattggttt tgcctcgccg
     4201 atgatcagga tatcataccc gattttttta tcggacgcat tccggtgatc aatgcgacac
     4261 aacttaatgc ctacattgat aaaatcattg cgtacgaaaa aggcccctcg ggcaattgga
     4321 aaaataaaat tgtgtttatc ggtggcagtg aggagcaacg cgcgacgttg ggaaaccctg
     4381 aaattccgcg cgacgtattc agataccaat ccactttact tatcaataac cgattggctc
     4441 agcgttttac acccgaacgc atctatgctt accccggcca agaatacgga ggcggtgcgg
     4501 gcgctgtaac tgattttttt agcgatggtg cacttatcat aacgtacctc ggccatggcg
     4561 gcggcggggt gtggggtgac cgcgatattg ccggtgaacc gatgatgaat cacgaccgca
     4621 tcaatctgat cacgcctaac ggcgggcgtt atcctatcgt cctgagcatg acttgttttg
     4681 tcggcgcttt tgacagcgac ggtcagtttg ctttgggcga aacaatgatg ttatcccgtg
     4741 atcgcggtgc ggccgcggtt ttagccgcca gcggaacagg ctggatcatc ggcgattatc
     4801 agatgctcga taatatgatg aacgaatttc ttgcacaaaa tacaaccgcc ggacaagcta
     4861 tcgccaaagg aaaaaccaat tatttgatct ttcgcggcga gcaggatttt tcggctgagg
     4921 gcgccaatcg ttcttccacg acctttcaat cgctcgtacc tcagagtatg gtatatcaat
     4981 ttaacttcct cggcgatccg gctttaaaaa ttcgtgcacc gcgcgctcgt acgatggcat
     5041 tgagtaacta cagtccgtct agaagcgctt cttttaacgt aagcggcgct accgattttt
     5101 caagcggttc cggaacagcg gagatttttc aaaccaaacc gacgaccgat tctgtttccg
     5161 gcggcgccaa taaatccagt tttgccaatg tcgtttcgat accgtttacc atcaccgacg
     5221 ggcagtatga tttttcgatc aatttgaatt cgatccccaa ttctgcgctt cgctccggat
     5281 tgacgggaat acggctttac ggcgaatcat cggacggtca aaaatacttc aacagtcaca
     5341 ccgattttta tattgacggt acttttatcg cggatatgaa aacaatacct gccgcaccga
     5401 ccagttcgga tattattcgt ttttcagcca cggtcagcga tcctcaaggt gttgcacgtg
     5461 tcatagctca ttatacggta acaggtccga ttgcatcatc tggcgttgat acgctcacgc
     5521 tcacatccgg aaatacgtac gaaagccaag gcataggccc tttcgcagaa aacgacaaga
     5581 tcacatacgt tcttaccatt tatgatacca atggcgattc cacacaaagt ccgtcgccta
     5641 attttaaaat tctgtcgggc attgatttaa acattcccac gccgctcacc aagtccattt
     5701 atgtcggcgg ttcatcggaa gtgcgcatta atgccgtcgt acaaaatctg ggttttgcca
     5761 aagctgagaa tgtgaatgtg cgattttatg aaggcaaccc gttgagcacc ggtgttttga
     5821 tcggtgaaac attcgtacaa gtagaaggca gcattaccaa ttccggcgtg atctcatccg
     5881 atacggcctc tattctttcc aatctgtcca acggagaaca tgtgattgcc gtttgggtag
     5941 atcccgactc cggtatcaac gatgtcagcc gctccaataa cttgcgcttt caaactattc
     6001 ggttaggtgc atttaatgtt acaccgtctt taggaactac gttcaacgga accaaaaatg
     6061 ataccgtgtc cgttgattcg gcactgcgta taagtattcc tcccggagtc tatactcaaa
     6121 acggtacgat gagtctcaat gtcaccggcg aatatacgat cgtcaatcaa ccggatatta
     6181 gccctgcgat tccgtacggt gcatcggctg cgcaagttta cgaaccgggc ttttcgcggc
     6241 ttatcgccga tggccaaccg atacatttga tcatgcgtta tgatacatta cgctataatg
     6301 cgacttatac tgattcgctg tccgtttacc gttgggactc cggaaatcgt aagtggacta
     6361 tcctctctgc ttcacaaact aaatcacgcg gccaaatctc ggcgatactg acttcaaatg
     6421 aagaccgtgc accgtggatg ctcatgatca atcgagatcg cactgcaccg gtgatcgaaa
     6481 ctacgatcga agggcaactt tttacgcaag gtggtattgc accgcgccaa ccgcgcgtct
     6541 cggctatagt ctatgaccgc aatggcgtaa gtctggatcg caaccactat gcgataactg
     6601 ttgataacgc accgatcagc gcttcacaaa tcgtattacc ggattcggta cccaattcaa
     6661 ataccgtcac atttacatta aatcttaacc aggcttttga tgacggccaa cacgaagtgt
     6721 tgtttacggc gaaagatgtc aacgggaata caagtaagac cgatacgctc aattttcagg
     6781 tcattcgtaa atttgacatc gaatcgtatg gcaattaccc caatcctttt atcaacacaa
     6841 caacgtttgc attccgtgtc gaagctccgg aacctttgga ggcactggat ataagcatct
     6901 acacggtgtc gggtcgtcgc attcgcaaaa ttacagacgc cgacgcgatt gcgtccggaa
     6961 aaaatattac agctgttggt tatcatgaag tgacctggga tgcgttggac gaagacggac
     7021 gcccaattgc caatggtgtt tatttttatc gtatacgcgg caaactcaat ggaaaaacta
     7081 tcgagaagaa aggtaaactg gcgtattttc gctgataacc cgatatgaaa aaaagtgttc
     7141 gaaccttaat attacttttc attagcgcag ctacggtcat cacggcacaa aaccgacgtt
     7201 acgccaatgc ctttttggaa atcccggccg gtgtcagagc ggcatcaatg ggtagtgctt
     7261 attcggcgat cgccaatgac gcgacggcat ttttttggaa tccggcaggt tccgtattgg
     7321 ttaatcgcca tgaggtcacg ggaagttatt cgagccagtt tggcgggctc gcgcagtatc
     7381 atgttttagg atacaatcat caaattaaca accgttattc tttcgctgta ggctggattc
     7441 gctatagcat cagcaatatt ccggaaaatg cggatctatc cggctcggct tcagaacatg
     7501 ctgtacccgg atttgatttt gcggcgctgc aacacggaca cttttcgtat gccgataatg
     7561 ctttcttttt tacttttgcg cgtatgaata ctctgcgtct taatctcgga tgggcctata
     7621 gtgacttccc tatacaaatt cccgttggcg caagttttaa aattatccgc ggcggtacaa
     7681 gcggcatctc cggagaaaac ggcgtggttc tcaacgatgt acgtaagtcg ggcatcggcg
     7741 tggatatcgg aacgatgatc atgtttggca tgaatgatct tttggaaatt cccacgcttg
     7801 gtgattttgt aatgagtttc aatcttcagg atgccacgac gacagccgtg cagtataata
     7861 aaatttcatc cgacgtacgc gctcaggata tcgtcaaagc taatttcaaa tttggcatgt
     7921 cgtacatcca aggattggat gaatggaaga gcaacatact tttatcgtac gaaagtaata
     7981 cgcgctacgc caacgatcgt cactacggcc ttgagtggga atacaataaa ttagctgcgc
     8041 tgcgcctcgg tatagacaac ggtactttga cttacggtgc cggatttcag tacaatcaca
     8101 tccacattga ttacgcattg aataatcacg cgttgggata cacgcatcgt atcggtactt
     8161 cgtataagtt ttagttcata aaggatgatc atgtttcgca acagcatttt atcgcttaca
     8221 tttatcggct tggctacaat gatatttacc ggatgtccgg acgaagtcaa tccgccggag
     8281 ccgcgtcctt ccaaagtgcg tatcaaagca cataacggcg atacgctggc ggttgatcgc
     8341 ggtacacgtc cgatcgaagg cgctttacaa ttgcttcgtt tagaatggtc accggtcacc
     8401 gactctgata atcttatcac ggaatatatc gtgtaccgat ccggcggttt agacagcgcc
     8461 tttgcagaga tcgccgttcg tactgtaaat gaagccgaaa ccgattcgtt tatcgaagac
     8521 gccgtgatcg ccgaaaaaac gtattactac tatgtcgtcg caaaagattc taaaggtaat
     8581 accaccgata tatccgatta tctagatgaa gaaaatctgt acacatatgt catgcctttc
     8641 cgtcttgcac aaaaagcctc cccgagacgt ccgtataatc aggatacggt ggtgaccacc
     8701 aaaccgcaat tctcttggtg tatttttggc gatgaacccg ggtattatgt gatcaaagtc
     8761 gcgacgccct ccggtcaact cgtatggctc gcttatcagc agcccggaac gtttggtgaa
     8821 aattgcggcg cgcaagaatc catgacactc aacaaacaaa cctattctta tattgatgaa
     8881 ttttttgacg gtattccggg cgattcactc tacgctacgg attccccgac ccgcgtcgta
     8941 tatgccaatc caaatttctt tgagggcgct cggcttcaaa aaggggttta ccaatggcgc
     9001 gtggatagtt atttcggtaa aaacaatgct tcccgatcgc cgtgggtgtc ctttaccgta
     9061 aatcgcgatt ataaaaatcc ttaaaagcgc acgcgttgat tttaatcgca aaatccttta
     9121 gtttgtttta ctaactaaag gattttttta tgtccatcat tgcttcgata cgcgaacgtg
     9181 cccgtcacaa aaaatgccgt attgttcttc ccgaaggcga agaagatcgc acattgcttg
     9241 ccgccaaaat cataataaac gaacagattg ccaaaccgat tttactcggt gatgccgaca
     9301 agataagaaa tagagcacgg gaattgcagg tggacttgcc ctcatcggtt tcgatcattg
     9361 atcccaagcg ttctgaaaaa acggcggatt atgcttcgga attatttaat ctccgcaagg
     9421 ccaaaggcat gacttacgag caggccaaac aacacatgca ttctgtttta tattatggcg
     9481 cgatgatggt acggcgtagc gaatgtgaag gttttgtcgg aggtgccgta tccacaaccg
     9541 cggatgtgtt gcgtgcggcc atacattgcg tgggcacggc gcccgatatc aatacggttt
     9601 cgagtatttt tttgatggta cttccggatg gacgggccat tacgtacggc gactgcgccg
     9661 tcatgcctta tccggatgcc gctcaactgg cggatatcgc catcgcatcc gccgaaagtc
     9721 acaaacaact caccggtgaa gaaccgatgg tggcgatgct ttctttttca acaaaaggta
     9781 gcgccaaaca tgaagccgtg gataaagtag ttcaggcgca ttctatcgtc cgcgaaaaaa
     9841 gaccggatat aaaaattgac ggagaattgc agtttgatgc agcatttatc gaatccatcg
     9901 gccagcgcaa agctcccggt agcgccgttg ccggtaaagc gaacgtattc atttttccta
     9961 atcttgatgc aggtaatatt tgctataaag taaccgagcg catcggtaag gcacaagcca
    10021 tcggcccggt tattcaaggt ttagccaaac cgatcaatga tctttcccga ggttgtacgc
    10081 cggaagacat tgccaatgta acggccatct gcgcgctcaa agcgtaaaac gtatactaaa
    10141 acaaaaaaac cctgcagaaa aaacttcagg gttttctatt tgtatttttt ttaattctat
    10201 cgtgctgccg cggccataat agtttcgacc tgaattcccc cgcgacgttt ttcgatcata
    10261 gttttagcat cccgagcaat ggaagccgga tcaagtccta cgcgttcgta taatacatca
    10321 ttatcgccat gctccacgat cgcatccggt aagcccaggc gcttaaggcg caaacgggcc
    10381 atcttttgta caaaatcttt atcttgtttt tggccgcgct caataaaatc ggaactatat
    10441 atcgtttcca atacaccgct gccaaaacca ccggttgtaa cattatcttc gatcgtgatc
    10501 acatcatcaa aatcacggca tatttcaaaa agcatcgctt catccaatgg tttgacaaat
    10561 cgtatattga ccaccgtagc cgaatagccg ttgcgaagta actcctgcgc cgcactaaga
    10621 gcatgttgga ccatgtttcc tacggccaat aaagcgatat ccttgccgcg ttgtacaaat
    10681 tccgattgac cgatcggaat tttttcaaat gtttccgtaa ccgtcacacc gataccgttg
    10741 ccacgcggaa aacgcacagc aataggtcct ttatcgtaca tcagcccggt gtacaccata
    10801 tctttcaact cgttttcatc tttgggtgcc atgatgacca tgccctgaat gcaacgcaaa
    10861 tacgataaat ccaacgcgcc gtgatgtgtc ggcccatccg cacccactaa acctgcacga
    10921 tcaaggcaaa ataaaacagg aattttctga atggccacat cgtgtacgat ctgatcgtac
    10981 gcgcgctgca aaaatgtgga atagatcgca acaataggtt tgactccgtt gagcgacatc
    11041 gctgcggcca ttgtcactgc atgttgttca gcaatgccta cgtcaaaaaa tcgctccggc
    11101 atttctttag ctaaaaattt taatccggta ccgtcggcca tagcggccgt aataccgaca
    11161 gcatgcgaat gcatcttgca aagcttgact accgttttac cgaaaacttc ggtgtacgtc
    11221 ggcgctgcaa cggtttccga agcgtttact tttttcggcg gagagacggc gtgaaattta
    11281 ttggcgttga ctttatattt ttccacatcc tgctcttcca aaggcatacc ttttcctttt
    11341 ttggtcaggg tatgaatcag aatcggacct ttgagatctt tgacatcacg caaaactttg
    11401 ataagacgcg gaagatcatg gccgtcaatc ggaccgaaat aacggaatcc gaggctttca
    11461 aaaaaatgac ccggcacaag catggacttg atactttcgt ccattttgct gagaccgtgg
    11521 cgtacgcgat caccgacttc gtgcaaatga ccgagcgcat tccacacttc cgtttttact
    11581 ttattataca acggtgctga aataagatcc gtaagataat tggataaagc tccgacattg
    11641 gaagaaatgg acatcatgtt atcattgagt acgacgatca tgtcggtttt aagcgaaccc
    11701 gcattattca tggcttcaaa agccattccg ccggtgagcg aaccgtcacc gatcacagct
    11761 acgactttgt tgtttttttt caattgatcg cgcgcgatcg ccataccgac ggccgccgaa
    11821 atcgaagtac tggcatggcc tgcaccgaaa gcgtcgtatt cactttcatg aattttacaa
    11881 aatccgctga tgccgtccgt ttgtcgtatc gtgtgaaatt gatctttacg tcctgtgaga
    11941 agtttatgca cataggcctg atgccccaca tcccaaataa ctttatccgt cggcgtatca
    12001 aatacatgat gcagtgcgac cgatatttct acgacgccga gactcggggc gagatggccg
    12061 ccgatacgtg aaatttcatc aatcacatag gcgcgcagtt cgcctgcaag ttgcactaat
    12121 tcatcggacg acaacgattt aatatcttta ggaagattca ctcgatcaaa gatcttggac
    12181 atgccattcg ctccaaacag tggtaaacaa agccttagaa gcttttatct ctaaagctaa
    12241 cacataacgg gcgcaaatta cgtatagcgc tataggaatg caacgaaaaa ctaattacag
    12301 aagcagcggc ccattcagta ctcaaatcgc tttttctatg gaaatccagt gataatagct
    12361 gtgcgttagt atgacttaaa gaaagactgt ttttgtgatg gcgcacgctt agaaaaaata
    12421 tttggacttc gcccgcgact ttaatacatt taaaaactaa tctatctgtt ctcaacggaa
    12481 tacatctaac tactgaacgg gagcatggca tgacgccgat ttcatcatcg cttttccgca
    12541 agaccctcgc tattattttg gccggcgg
//
LOCUS       DAOQGF010000098        12560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2333_length_12560_cov_6.353059, whole genome shotgun sequence.
ACCESSION   DAOQGF010000098 DAOQGF010000000
VERSION     DAOQGF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_12560_cov_6.353059"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1290
                     /locus_tag="PK937_12180"
     CDS             148..1290
                     /locus_tag="PK937_12180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH33375.1"
                     /translation="MDFFDLFLWYSAGLYFFGLTVLAIGFCLYKPRRASSYNPLVSVV
                     IAARNEEVHIDRCLAALSEQTYPADRMEIIVVNDRSTDGTENVVQTWSKKIPHLKIVS
                     IREASKSMAPKKFAVDSGIRNSKGEIILCTDADCRPERAWVATMIEHFTPEVGMVLGY
                     SPLWPVHPNSLFENFVACESIGLGVVAASTTALHKPWTVTGRSWGYRRGVYDEVGGFT
                     KIALQVSGDDDLLLGLMKITKWKTAYAIGEQANVRTDPPKSINHFRNQKTRQLSKTRL
                     YSFKMQISVMWYYLFNVALIVSLFFAVLYSEQWRTVVWAWSLKMFSDVIFLSAGAYRF
                     RVLLFLKYYPFLVLIHPVYVLTFGLLGRFGRYTWKDTPVAQGEARS"
     gene            1287..2402
                     /locus_tag="PK937_12185"
     CDS             1287..2402
                     /locus_tag="PK937_12185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH33376.1"
                     /translation="MIEMILFIVMGVYFASYVFGVMWILMGLFFPHRVFSKKQPKVSV
                     IVAARNEADNIRYCAESLAAQDYPKDQYEIILVNDRSEDQTLAIMQAFVKKFPEQFRI
                     VSVQDKPLDASGKKYALKQGIEAAVGEILLFTDADCTVGPQWITTMASYFSDKTDFVI
                     GFSEVRAVTTFERWQRTDFLAMMIAAAGVSNLGMPWSASGQNLAYRRKAYDAVGGLDP
                     IMHRISGDDILMMNRMRQNGAGIVFAMHPGAYVQTEPVPDWSTFMRQRARWASNGPIM
                     LRYNLWFFCYLTIIYGVCLGLITSLAAGIFLKPWLWLGGFMLVVKAIVDSAAMMIGSR
                     QLQKKVSPTDFIYWFAVQPFYVVWSGMRGMLGIFTWK"
     gene            complement(2550..3431)
                     /locus_tag="PK937_12190"
     CDS             complement(2550..3431)
                     /locus_tag="PK937_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33377.1"
                     /translation="MRALLTTTMLTVSLCLTSCGNWNIGKQGKPYGFSGFATVRHDAK
                     ETVLLTVHDNKKSGEPRLGLVTMAEKKPVKYEGLVWPDSVNIPIDGEALCKYPIGENT
                     YVVMSSMGKCFLFTFDQASKQIAVTGRFDLPEAPKGSNFEGLDFRLLNDTAMAAIWGH
                     RGQDEDPAVIYSGVFNPRTMKFANILSLPFKTPFPKKKVRYISDMKMDEAGTLYVVSS
                     SDTGDNGPYESAVYIVGNIRFNGTQASITSNSQYVRLFTDTKHKIEAMELVPGEFGGI
                     IYGTDDENKGASIQFVK"
     gene            complement(3512..4534)
                     /locus_tag="PK937_12195"
     CDS             complement(3512..4534)
                     /locus_tag="PK937_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNH33378.1"
                     /translation="MPIPWFRQFEKADTPKSTLHGESQPFVSYYDDPETEYRRAREQA
                     ILIDTSFYGTLQLQGRHTLSLLHRLTTQDIQNMTSNTVQQTVLTSDRGRIVDLLRVFH
                     GDQSTTLRTSAERSATIAQWLDKYIIMDDVRINDISDSTSSVFLIGPRAAQVLIQIGI
                     TIPDTNVFLKEGDIIYTQCAAYPNNGFEFFGPTSVIEALCEKLLPLAQPCGRMTYETV
                     RIEDAIPAYHFELSENYNPLEAGLVGAISFTKGCYIGQEVIARLDSREKVQRQLITFK
                     TEKEVAYADKVFWGNKEIGLITGACYSYRHRSPIARAYVKTDSLTDDTAFVVMSGSEK
                     VTAARI"
     gene            4689..7208
                     /locus_tag="PK937_12200"
     CDS             4689..7208
                     /locus_tag="PK937_12200"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNH33379.1"
                     /translation="MAKLQSKERQKKSETSTATGLQIKPWQQTAISILILYALNVFLF
                     SDIVIKGKAFSSGADNSAATAISRGMGELVKQEGDLPLWNPFMFAGMPSFASGMYGNP
                     DATPLIKYQKYFNPSYYVSLVVNLFFGNRDNVWEVATFFLAGVFMFLLARSLGFGHLI
                     ALTAAVSYMFCNFFVASMAAGHGGKVRTIAYIPLVVWSVFNFFQNRSVWTWAIMGLVL
                     GLFFQEAAHTQIIYYAFLMLGIYCVVYLVDEFRNNLSGVIRNGLGFTAALITGLGFGA
                     LGQIALYVYSDVTMRSMGPAFAETTEMAGAGGMTFDYITMWSFHPLESVTFFIPGFFG
                     LESPYYWGWMTFTSSAFYIGLLPMVAAVFGILYRRNTITKTMVFTALLALLIAFGKFF
                     EPFFKLMLSILPFFEKFRVPSMILTLFIFAVTVLSCYGLDYIFNPTEEERKRRGGLEK
                     VLLYVMGGIAVLGVVSLLMPDFWSGMAGFLADGDAQRYNANQIAQLKKLRLDALNSGV
                     IRFAVLGVIIFGALYLYMKEKIGAGLALMIMLMVNITDTISLNMKTLRPVSRAATQQE
                     FQETETIRFLHSDTTRFRIFSLLEHAQSGSPVWNYFGIESLSGYSPAKMRIYQDVIEF
                     SMYKGPDPQFPINMNVVNMLNAKYVLANGRLPEGMGFLMRKIDEAGKTLIYENKNVLP
                     RAYFVSSVETIGERQQALLRLNSVDFDVHRSAFTDKAIKTPITPFDSAKAEITQWMTD
                     KISISAFVDKPGFMVLSETYYPHGWKAYVDGQETEIYKTNYILRGIQVPAGQHTIEFR
                     FEPTEYRAGIWASTISFFGVIGLLVVGGVMQYRRLQNEPKP"
     gene            7240..8283
                     /locus_tag="PK937_12205"
     CDS             7240..8283
                     /locus_tag="PK937_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33380.1"
                     /translation="MKMFWFFLFSVLTVSSSQAQEEYMTQTLNLKVYVKLINDVQPLL
                     DKTIEETGSRILSSTQGSLQYNTTANSLRMTIWSSAAGFVKLQQTIKSLGLVRHHEIT
                     TVNNADILASLEMELDHLNKKKQSYESEISRIDASSEKHGFLWDELRTIEDRIFETER
                     SILEKKQQTSNYVIELEVIEESYAPFEDAQDVQFVNMPGFEYAELRIENPLNTVSHKT
                     YRGGMVKYLFTKGKSYVRFGVLRSGQDGATPDEIEELFFYSFGQDFYPRYFGRGARSF
                     ANLYTGYSTGGVFATADDRRKNVFFLMPHIGLELYKTRFILWDTQAGYFIPFYQNRNF
                     RGIAGHMSFNFVF"
     gene            8291..10804
                     /locus_tag="PK937_12210"
     CDS             8291..10804
                     /locus_tag="PK937_12210"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNH33381.1"
                     /translation="MKIWVRRTLFGLTALLLVLTGLVLFGYFAARKSLPQTEGQLSLR
                     GLSAPVTVYRDEFAVPHIDASGETDLMRATGYVQAQDRLWQMDLLRRIAEGRLSERFG
                     TKTLSVDKALRTIGFMRMATMLKDSLPPDVMALLQAYADGVNAFIEENKGNYPPEFTL
                     IDFTPEAWKPEHSIALSRLLGWQLSMGWHVDVTYDRILDTVGFAKLQEILPRFPADAP
                     TIVPMVPPDYNAKSVLNGISENTYHEEHFRPAPKAAAAELRHFADALYEVKRIIGIEG
                     FSVGSNNWVVSGARSVSGKPLLANDPHLGHAMPPTWYEMHLRGGRLDVAGFTLPGVPL
                     IVLGNNNRIAWGFTNVMMDDADFYREQIRDSMYYFNGAWRKLGWAEEKIVIYDSTDVV
                     FRIPMTHRGPIVQGFYANVSGSEAISVRWSGYEATREVEAMLGMNIAQDWTGFRKAAS
                     EFKIPAQNIVYADVDGHIAYQCVGNIPLRRNGNGISILDGSVLQNDWAGYLPFESNPY
                     RLDPPEGFIASANNKTAGDWFPYFISNYWEHPSRIKRIHDFMNGQEKFSPADFQKFQN
                     DFYSYHALEVIPYILEACSKDSNFTKQDLEHVSNYRYNESYLFLKHWDGRMHADSRGA
                     AIFNVFFRRLTRNLYSDEMGDDLYRDFVKLTNIPTRVTTQLLQLPKSAWWDNVRTREI
                     EDRDAILRITMTETVDELTADLGSEPGGWAWGKIHHVEFEHFVGRQKPLNYLFNVGPF
                     AVGGNTTTINNTEFNYSDSTYKAVIGASMRRIVDLADIQHPKTVLTVGQSGQPFSLHY
                     DDQVRMWLDGQYKIINLDWGALRNNPSHHRLILMPPNAK"
     gene            10849..11322
                     /gene="ispF"
                     /locus_tag="PK937_12215"
     CDS             10849..11322
                     /gene="ispF"
                     /locus_tag="PK937_12215"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNH33382.1"
                     /translation="MRIGQGYDVHRLAEGRKMILGGVEISADKGPLGHSDADVLLHAI
                     CDALLGAIGLGDIGKHFPDTDQAYKNISSLILLEKVYALITERNYRVGNVDATLVLQK
                     PKIAPHVPAMKKNIAAVLHVEEERVSIKATTSEGLGFVGAGDGVVAYAVALVFKS"
     gene            complement(11323..12555)
                     /gene="glgC"
                     /locus_tag="PK937_12220"
     CDS             complement(11323..12555)
                     /gene="glgC"
                     /locus_tag="PK937_12220"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNH33383.1"
                     /translation="MSSSLFRKTLAIILAGGQGERLYPLTKDRSKPAVPFGGKYRIID
                     FALSNAINSGFRKIYLITQYKSQSLDTHIREGWSFLNGELGEYIYCIPPQFRTANHWY
                     QGTADAVFQNLHVLQDDKPEYVIILSGDHIYKMDYLKMLQYHIAKQADLTIAGIEFQR
                     DQAHQFGVMVVNEDNDIIEFQEKPKEPKPLPNKPDFCYVNMGIYIFSADAMAHVLVDD
                     AKLKDSAHDFGKNVIPSMLGKYKVTAYNFEDENKAGQPYWRDIGTLDSYFDTSMDLIS
                     VSPTFNLYDIDWPIRTYQGQYPPAKNVFSEPQEGGRMGVALDSIVSGGVIISGGRVTR
                     SLLSPNVRIHSYSSVDESILFNNVSVGRNSKIKRAIIDKNVVIPENSEIGYNLEEDKK
                     RFFVTPSGIVVISKNQRI"
BASE COUNT         3210 a         2562 c         3150 g         3638 t
ORIGIN      
        1 agggtgatca atgcgcaagg taaaatttct tttcctaaag gaagccggca ttacgagcgg
       61 caaaaaaaaa tgcttaaaac cgaaggtatc gagtttaatg gggaaaaaat aaacctcgat
      121 cgttttggtt gggatccgga gtagggaatg gatttttttg atttgtttct ttggtattcc
      181 gcagggttat atttttttgg attgacggtt ttagccattg gtttttgttt gtataaacca
      241 cgccgtgctt caagttataa tcctctcgtt tctgtcgtta ttgcggcacg taatgaagaa
      301 gtccatatag accgctgttt agcagcgctt tcggaacaga cgtatcctgc cgatcggatg
      361 gaaatcatcg tggttaatga ccgttcgacc gacggaacag aaaatgtcgt acaaacatgg
      421 tctaaaaaaa ttccccattt gaaaatcgtc tcgattcgtg aggcatccaa gtccatggcg
      481 ccaaaaaaat ttgctgtgga tagcggaata cggaactcta agggtgagat aattctgtgt
      541 acagatgccg attgccgacc ggagcgtgcg tgggttgcaa ccatgatcga acattttaca
      601 ccggaagtgg gtatggtgtt ggggtattca ccgttgtggc cggttcatcc gaattcgtta
      661 ttcgaaaatt ttgttgcttg cgaaagtata gggttaggtg ttgtagccgc ttcgactacg
      721 gcattacata aaccatggac cgtgacaggt cgtagctggg gttatcggcg tggagtgtat
      781 gatgaagttg gcggttttac aaaaattgca cttcaggtgt caggcgatga cgatttattg
      841 ctgggtttga tgaaaataac aaagtggaag accgcctatg cgatagggga acaagccaat
      901 gtccggactg acccgccgaa aagcataaac cactttcgta atcagaaaac acgccagtta
      961 tctaaaaccc gcctgtattc gtttaaaatg caaatcagtg tgatgtggta ttatttgttt
     1021 aatgtggcgc tgatcgtatc actttttttt gcggttttgt attctgagca atggcgtacc
     1081 gttgtttggg cttggtcact gaaaatgttt tcggacgtta tatttttgtc ggctggtgcg
     1141 tatcggtttc gagtcttatt gtttttaaaa tattatccgt ttcttgttct aattcatccg
     1201 gtatatgtat tgacgtttgg tctattagga cgatttggcc ggtatacctg gaaagatacg
     1261 cccgtggcac aaggagaagc gcggtcatga tcgaaatgat attatttatt gtaatgggtg
     1321 tttatttcgc gagctatgta tttggcgtga tgtggatact tatggggtta ttttttccgc
     1381 atcgtgtttt ttcaaaaaag caacctaaag tatccgtgat cgtagctgcg cgcaatgaag
     1441 ccgataatat tcggtactgc gcggaaagtc tagctgccca ggattatccc aaagatcaat
     1501 atgaaattat attggttaac gaccgatccg aagatcagac attggcgatc atgcaagcgt
     1561 ttgttaaaaa attcccagag caatttcgta ttgtatcggt tcaggataag cctctggatg
     1621 cgtccggaaa gaaatatgct ttgaaacaag gtattgaagc cgcggtcgga gagatacttt
     1681 tatttactga tgccgattgt accgtcggcc cgcagtggat aacgacgatg gcgtcttatt
     1741 tttcggataa aacggatttt gtcatagggt tttctgaagt acgtgcagta acgacatttg
     1801 aacgttggca acgcacagat tttttagcca tgatgatcgc ggctgcgggc gtgtccaacc
     1861 tgggaatgcc gtggtcagcc agcggacaaa atctggctta tcgtcgcaag gcctatgatg
     1921 cggtcggcgg tttggatccg atcatgcatc gcatttcggg ggatgatatt ttgatgatga
     1981 atcgtatgcg acaaaacggt gcgggtattg tctttgcaat gcatccgggc gcttatgttc
     2041 agacagagcc ggtaccggat tggtcaacct tcatgcgtca gcgcgctcgc tgggcatcca
     2101 acgggccgat catgctgcga tacaatcttt ggtttttttg ttatctcacg atcatatacg
     2161 gggtctgcct tggacttatc accagtttgg cggcgggtat ttttttgaaa ccgtggctat
     2221 ggctgggcgg atttatgcta gttgttaaag ctatagtgga tagcgcagcc atgatgatcg
     2281 gatcaaggca attacaaaag aaagtatcgc cgacggattt tatatattgg tttgccgtcc
     2341 aaccttttta tgtggtatgg tcaggcatgc gcggtatgtt ggggatattt acctggaaat
     2401 gaaaagaata tctcgatctg aagggttttt gtaccaacac gtacaagatt tataaacgtt
     2461 atacttttct catatcgtat ctaaataaac aaaagacaca ctagctcaat agatcgtacc
     2521 tgattacagt ctattaaaat aatttataat tatttcacaa actggatgga agcgcctttg
     2581 ttttcatcat ccgttccata gataatgccg ccaaattcgc ccggtaccaa ttccatggct
     2641 tcgattttat gtttggtatc cgtgaaaaga cgaacgtatt gcgaattgga agtgatagag
     2701 gcttgtgttc cgttaaagcg aatattgccg acgatataaa cggccgattc ataagggccg
     2761 ttatcacccg tatcggatga cgaaacaaca tacaaagttc cggcttcatc cattttcatg
     2821 tcggatatgt aacgcacttt tttcttgggg aacggtgttt taaaaggtag tgaaagaata
     2881 ttagcaaact tcatggtgcg tggattgaat acgccggaat aaataacagc cggatcttca
     2941 tcttgaccgc gatgccccca aatagccgcc attgctgtat cattgagcaa acggaaatct
     3001 aatccttcaa aattgctgcc tttgggcgct tcgggtaaat caaaacgacc ggtaacggct
     3061 atttgtttgg aagcctgatc aaaggtgaaa agaaaacatt ttcccatgct ggacataacg
     3121 acgtacgtgt tttcaccgat cggatattta cacagcgctt cgccgtcaat ggggatattt
     3181 acggaatccg gccaaaccaa accttcgtat ttcacaggtt ttttttcggc catcgtaacc
     3241 aatcccaaac gcggttcacc gcttttttta ttgtcatgca cggtcagcaa aacggtttct
     3301 ttagcatcat ggcgcactgt ggcgaaaccg ctgaatccgt agggttttcc ttgtttgccg
     3361 atattccagt taccgcaaga cgtcaggcag aggctgacgg tgagcatggt agtagtcaac
     3421 aacgcgcgca tgtgattcct cataagtgag atgaaaagaa tttgtcaatg tagcggaatc
     3481 cctgctgaaa aacaaaatat ttttttgaga actatatacg tgcggccgtt actttttcag
     3541 aacccgacat gacgacaaaa gcggtatcgt cggttagact gtcggttttg acatacgctc
     3601 gtgcgatggg tgagcggtga cggtaggagt aacaagcgcc cgtgattaaa ccgatttctt
     3661 tgttacccca aaaaactttg tcggcataag cgacttcttt ttctgtttta aacgtaatca
     3721 actgacgttg tactttttca cggctatcca gacgtgcaat cacttcttgc ccgatataac
     3781 accccttggt aaaactgatg gctcctacca atccggcttc caacggatta taattttccg
     3841 ataactcaaa atggtacgcc ggtatggcgt cttcgatgcg tacggtttcg taggtcatgc
     3901 gcccgcaggg ttgagccaaa ggaagtagtt tttcgcacaa cgcttcgata acagaggttg
     3961 ggccaaaaaa ttcaaatcca ttattgggat acgccgcgca ttgtgtatag atgatatcgc
     4021 cctccttcaa aaatacattc gtgtccggta tggttattcc tatctgaata agaacttgtg
     4081 cggctcgggg gccgatgaga aatacagagg aagtgctatc ggaaatatca tttatccgaa
     4141 catcgtccat aatgatgtat ttatcaagcc attgggcgat cgttgcggac cgctccgccg
     4201 atgtgcgaag tgtcgtactc tgatcgccgt ggaatacgcg caataaatcc acgatgcgac
     4261 cacgatcgga agtgagtacc gtttgctgta cggtatttga agtcatgttt tgaatatctt
     4321 gcgtggtcaa acgatgtagt aaactcagcg tgtgacggcc ttggagttgg agtgttccgt
     4381 aaaaagaagt gtctatcagg atcgcttgct cacgcgcccg tcggtattcg gtttccggat
     4441 catcataata cgatacgaat ggttgcgatt ctccgtggag cgtgcttttc ggagtgtcgg
     4501 ctttttcaaa ttgtctgaac catggtatag gcattatcgt cgctttctat tcatattttg
     4561 cgcggcaata tacttcatcc atacgaacag cgcaagcatg tccacggttt gaggtaaatt
     4621 ctcttttgat attccttcgt ttttcgtaaa ctttgccgca ttactatcaa taaccattga
     4681 atcggattat ggcaaaatta caatcaaaag aacggcaaaa aaaatcggaa acctccaccg
     4741 cgacgggact tcaaatcaaa ccctggcaac aaaccgcgat cagtattctt attctctatg
     4801 cactcaatgt gtttcttttt agtgatattg tgatcaaagg gaaagccttt tcgagcggtg
     4861 cggataattc agcggctaca gcgatctccc gcggtatggg cgaattggtc aagcaagaag
     4921 gtgatcttcc gctatggaac ccctttatgt ttgcaggcat gcccagcttt gccagcggta
     4981 tgtacggcaa ccctgacgct accccgctga tcaagtatca gaagtatttt aacccttcgt
     5041 attatgtaag cctggtcgtt aatttgtttt tcggtaaccg ggataatgtg tgggaagtgg
     5101 cgactttttt tcttgccggt gtatttatgt ttttactggc acgcagtctt ggatttggcc
     5161 atttgattgc cctaacggcg gccgttagtt atatgttttg caattttttt gtcgcctcga
     5221 tggcggccgg tcacggcggc aaagttcgta cgatcgctta cattccgctg gtcgtgtgga
     5281 gtgtttttaa ttttttccaa aaccgcagcg tatggacgtg ggcgatcatg ggacttgttc
     5341 tagggttgtt ttttcaggaa gcggcgcaca cgcaaatcat ttattatgct tttctgatgc
     5401 tgggtattta ttgcgtcgtt tatcttgttg acgaatttcg taacaatctg tcgggcgtga
     5461 ttcgaaacgg tttgggtttc accgccgcgc tgataaccgg gttgggattt ggcgcacttg
     5521 gccaaattgc gctgtatgtg tattccgatg ttacgatgcg aagtatgggg ccggcatttg
     5581 ccgaaacaac ggaaatggcc ggtgccggtg gtatgacatt tgactatatc acgatgtggt
     5641 catttcatcc gctggaatct gttacatttt ttataccggg cttctttgga cttgaaagcc
     5701 cgtattattg gggttggatg acgtttactt cgtcggcttt ttatattggt ttgctgccga
     5761 tggtggccgc agtttttgga attttatatc ggcgcaatac gatcactaaa acgatggtat
     5821 ttacggcgtt gctggcattg cttatcgcat tcgggaaatt ctttgagccg tttttcaaac
     5881 tcatgctttc gattttgccg tttttcgaaa agttccgtgt tccatccatg attctcacgc
     5941 tttttatttt tgccgtcact gtactgtcgt gctacggttt ggattatatt tttaatccga
     6001 cggaagagga acgcaaacgc agaggcggtt tagaaaaagt tttactctat gtcatgggcg
     6061 gcatagctgt actgggtgta gtttcattat tgatgcccga tttctggtcc ggtatggccg
     6121 gttttttggc cgacggcgat gcgcagcgat ataatgccaa tcagatcgca caattaaaaa
     6181 aattgcgttt ggatgcactc aatagcggtg taattcgatt tgccgtgctg ggtgtgatca
     6241 tattcggtgc gttgtatctt tatatgaagg aaaaaatcgg ggctggccta gcattgatga
     6301 taatgcttat ggtgaatatc accgatacga tcagtctcaa catgaaaacg ctccgtccgg
     6361 tttcgcgtgc agctacgcag caggagtttc aggagacgga aactattcgt tttcttcact
     6421 cggatacgac gcggtttcgt attttttctt tgcttgaaca cgcccaatcg gggtcgccgg
     6481 tgtggaatta ttttggcatt gagagtctga gcgggtatag ccctgctaaa atgcgtatct
     6541 atcaggacgt catcgaattt tcgatgtaca aaggtccgga cccgcaattt ccgattaata
     6601 tgaatgtagt caacatgctt aacgctaaat acgttttggc caacggacgt ttgcctgaag
     6661 gtatgggttt tctgatgcgt aaaatagatg aagcggggaa aacgctcatt tatgaaaata
     6721 aaaacgtttt accgcgggcc tattttgttt catccgtaga aacgatcggc gagcgccagc
     6781 aagccttact gcgccttaac agtgttgact ttgatgtgca tcgctcggca tttaccgaca
     6841 aagcgatcaa aacgccgata acgccgtttg attcggctaa agcggagata acacaatgga
     6901 tgacagacaa aatttcgatc tcagcgtttg tagataaacc gggatttatg gtgctgagcg
     6961 aaacatatta tccgcatggc tggaaagctt atgtggacgg acaggagacg gagatttata
     7021 aaacaaatta tattctgcgc ggcattcagg taccggcggg acaacatacg atcgaattta
     7081 gatttgaacc gactgaatat agggccggta tttgggccag cacgatttcg ttttttggtg
     7141 tgatcggatt attagtggtc ggcggagtta tgcaatatcg ccgactgcaa aacgagccga
     7201 aaccttgatc tgggatttat tttaaaaaga ggtgttatca tgaaaatgtt ttggtttttt
     7261 ttattttctg tgctgaccgt aagttcgtcg caagcacaag aggagtatat gacgcaaact
     7321 ttgaatctca aagtgtatgt caaactgatc aacgatgtcc agcccttatt agacaagacc
     7381 attgaggaaa cgggaagtcg tattttgtct tccacgcaag gcagcttaca atataataca
     7441 acagctaatt cgcttcgaat gaccatttgg tcttcggcgg cggggtttgt caaattacaa
     7501 caaacgatca aatcactggg tcttgttcgc catcacgaga ttaccacggt caataatgcg
     7561 gatattctgg catcgcttga aatggaattg gatcatctca ataaaaagaa gcaatcgtat
     7621 gagagtgaaa tcagccgcat agatgcgtcg agtgaaaagc acggtttttt atgggacgaa
     7681 ttgcgtacga tcgaagatcg gatatttgaa acagagcgat ctattttaga aaagaagcag
     7741 cagacatcca attatgtgat cgaactcgag gtcatcgaag aatcatatgc cccgtttgaa
     7801 gatgcgcaag atgttcagtt cgtcaatatg cccggttttg agtacgccga gctgcgtatc
     7861 gaaaatccgc taaatacggt ttcgcacaaa acgtaccggg gcggcatggt caaatatctc
     7921 tttacaaaag gtaagagcta cgttcgtttt ggtgttttac gcagtggtca ggatggtgca
     7981 acgccggacg aaatcgagga gcttttcttt tacagttttg gtcaagattt ttatccgcgc
     8041 tattttggcc ggggcgcacg ttcgtttgcc aatctatata cgggttatag cacgggcggt
     8101 gtttttgcga cagccgatga ccgtcgaaaa aatgtatttt tcctgatgcc gcatatcggc
     8161 ctggaactgt ataaaacacg ttttattttg tgggataccc aagccgggta ttttatcccg
     8221 ttttatcaaa accgcaattt tcgcggcata gcaggtcata tgtcgtttaa ctttgtattt
     8281 tgagatcact atgaaaattt gggttcgccg tacgctattt ggtttaaccg cattgttact
     8341 ggttctgaca gggcttgtac ttttcggtta ttttgctgcg cgcaaaagtt taccgcagac
     8401 cgaggggcag ctttcgctca gaggattgtc ggcaccggtg acagtttacc gtgatgaatt
     8461 tgccgtaccg catattgatg cgtctggaga aacagacctg atgcgggcga cgggttatgt
     8521 gcaagcgcag gatcgtttgt ggcaaatgga tttgctccgt cgtatcgccg aaggacgctt
     8581 gagcgaacgt tttggtacaa aaaccttaag cgtagataaa gcgctgcgta ctataggttt
     8641 tatgcgtatg gcgaccatgc tcaaagacag tttgccaccc gatgttatgg cgcttttaca
     8701 ggcttacgcg gatggcgtga atgcttttat agaagaaaat aaggggaact atccaccgga
     8761 atttacactg attgatttta ccccggaggc gtggaagcct gagcattcga tcgcgctgtc
     8821 acgactactt ggctggcaac tgagcatggg atggcatgtg gatgtcacat acgatcgtat
     8881 tttggatacg gttggttttg ctaaattgca ggagattctt ccgcgttttc cggcagatgc
     8941 accgactatc gtaccaatgg ttccacctga ttacaatgct aaatccgttt tgaatggcat
     9001 ttcagaaaac acatatcatg aagaacattt tcgccccgct ccaaaagccg cagcggcgga
     9061 actgcgtcat tttgccgatg ccttatatga agtgaaaagg attatcggca tcgaaggatt
     9121 tagtgtaggg agcaataatt gggttgtatc cggcgcacgg tcggtttccg gtaaaccgtt
     9181 actggccaat gatccgcacc tggggcatgc gatgccgccg acttggtatg agatgcatct
     9241 gcgcggagga agattggatg tagccggttt cacgctgccc ggtgtgccgc tcatcgtttt
     9301 ggggaataac aatcggatag cctggggttt taccaatgtg atgatggatg atgcagactt
     9361 ttaccgtgag cagatccgtg acagcatgta ttatttcaac ggcgcttggc gtaagctcgg
     9421 ctgggcggaa gaaaaaattg tgatttatga ttcgaccgat gttgtattcc gcattccgat
     9481 gacacaccgt gggccgatcg tacaagggtt ttatgccaat gtttcgggta gcgaagcgat
     9541 ttccgtgcga tggagcggat atgaagccac gcgcgaagta gaggcgatgc tgggaatgaa
     9601 tattgcgcag gattggaccg gttttcgcaa agccgcctcc gaatttaaaa taccggcgca
     9661 aaatatcgtg tatgcagatg tggacggaca tattgcgtat caatgtgtgg ggaacattcc
     9721 attgcggcgc aatggtaacg gcatttccat tttggacgga agcgtattac agaatgattg
     9781 ggccggttat ttaccttttg aatcgaatcc ataccgactt gacccgcccg aaggttttat
     9841 tgccagtgct aataacaaaa cggccggcga ttggttcccg tatttcattt ccaattattg
     9901 ggagcatccg tcgcgcatca aacgcattca tgattttatg aatgggcagg aaaaatttag
     9961 tcctgccgat ttccaaaaat ttcaaaatga cttttattcg tatcacgcat tagaagtgat
    10021 tccgtatatc cttgaagcgt gcagtaagga ttcgaatttt acaaaacaag atcttgagca
    10081 tgtatccaat tatcgctata acgaatcgta tttatttctg aagcattggg atggtcggat
    10141 gcatgcggac agccggggtg cggctatttt taatgttttt ttcaggcgat tgacgcgcaa
    10201 cctctattcg gatgaaatgg gggatgatct gtatcgcgat tttgtaaaat tgacgaatat
    10261 tccaacacgg gtgacaacgc aactcctgca acttccaaag tcagcatggt gggataatgt
    10321 acgaacccgc gaaatagaag accgcgatgc aatacttcgt attacgatga cggaaacggt
    10381 ggatgaatta acggccgatt tgggcagcga gcccggcgga tgggcgtggg gtaaaattca
    10441 tcatgttgaa tttgaacatt tcgtagggcg acagaagcca ttgaattatc tttttaatgt
    10501 cggtccgttt gcggtcggcg gtaatactac gactatcaat aataccgaat ttaattacag
    10561 tgactccacc tacaaagccg tgatcggtgc gagtatgcgc cgcatcgtag atcttgccga
    10621 tatacagcat ccgaaaacgg tcttgaccgt cggacaaagc ggccaaccgt ttagtctcca
    10681 ctacgatgat caggttcgta tgtggcttga cgggcagtat aagataatca atttggattg
    10741 gggcgctctg cgcaataacc cgtcgcatca ccggctgata ttgatgcctc cgaatgcaaa
    10801 ataaatttta taaaataatt tcactgtaaa cataaaggcg ttatggatat tcgtatagga
    10861 caaggttacg atgtgcatcg gcttgcggaa ggccgtaaga tgatattggg cggagtggaa
    10921 atttctgcgg acaaaggccc tttggggcac tcggatgcgg atgtactgct gcatgcgatc
    10981 tgcgatgcgc ttttgggggc tatcggcctc ggcgacatcg gtaaacattt tccggacacc
    11041 gatcaagcct acaaaaatat ttccagcctt attttacttg aaaaagttta cgcgttgatc
    11101 accgagcgaa attatcgtgt ggggaatgtg gatgcgacat tagttttaca gaaacctaaa
    11161 atcgcgccgc atgtgccggc tatgaaaaaa aacatagctg cggttttgca tgtcgaagag
    11221 gaacgggtgt ctatcaaagc cacgacctcg gaaggacttg ggtttgtcgg tgcaggtgac
    11281 ggagttgtag cgtatgccgt cgcgttggtt tttaaatcct gatcagattc gttgattttt
    11341 tgatataacg acgatgcctg aaggcgtcac aaaaaatcgt tttttatcct cttcaagatt
    11401 gtagccgatt tccgaattct ccgggatgac gacatttttg tctatgatcg cacgtttgat
    11461 tttcgaattg cgtccgacgc tgacattatt gaaaagaatg gattcgtcga cgctcgaata
    11521 actgtggata cggacattgg gtgaaagcaa ggatcgtgtt acacgaccgc cggaaataat
    11581 cacgccgccc gaaacgatcg aatcaagtgc cacgcccata cgtccgcctt cttgcggttc
    11641 ggaaaataca tttttagccg gcgggtattg tccctgatat gtgcgtatcg gccagtctat
    11701 atcgtacagg ttgaatgtcg gtgaaacgga aatcaagtcc atgcttgtat caaaataact
    11761 atccagtgta ccgatgtcgc gccaataggg ttgaccggcc ttattctcgt cttcaaaatt
    11821 ataggctgtc actttgtatt taccgagcat ggaagggatg acgtttttac cgaagtcgtg
    11881 ggccgaatct tttagtttgg catcatctac caaaacgtgc gccatcgcat cggcgctaaa
    11941 aatataaata cccatattga cgtaacagaa atcaggcttg ttcggaagcg gtttgggttc
    12001 tttgggtttt tcctggaatt cgatgatatc attgtcctca ttgaccacca tcacgccaaa
    12061 ctgatgggct tgatcgcgtt gaaattcgat cccggcaatg gtcaggtcgg cttgtttggc
    12121 aatgtggtac tgtaacattt tgagataatc catcttgtaa atgtgatcac cggagagaat
    12181 gatcacatat tccggtttat catcttgcag cacatgaagg ttttgaaata cggcatcggc
    12241 cgtgccctga taccagtgat tggccgtacg aaattgcggc ggaatgcagt aaatatattc
    12301 accgagttcc ccgttgagaa aactccagcc ttcgcggata tgcgtatcga gcgattgcga
    12361 tttgtattgg gtaatcagat atattttacg aaaaccggaa ttgatcgcgt tactgagggc
    12421 aaaatcaatg atgcggtatt tcccgccaaa cgggacagcg ggtttggagc gatctttggt
    12481 gagaggatat agacgttcgc cttgaccgcc ggccaaaata atagcgaggg tcttgcggaa
    12541 aagcgatgat gaaatcggcg
//
LOCUS       DAOQGF010000099        12508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2345_length_12508_cov_6.976391, whole genome shotgun sequence.
ACCESSION   DAOQGF010000099 DAOQGF010000000
VERSION     DAOQGF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2345_length_12508_cov_6.976391"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1063)
                     /locus_tag="PK937_12225"
     CDS             complement(83..1063)
                     /locus_tag="PK937_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNH33384.1"
                     /translation="MKHAPLKTDPIVKEIDKAIKVYLAKRNPTTLYDPARYLFQSGGK
                     RMRAVMTVLSCALFEKNYKKSFPAAAAVELLHNFSLVHDDIMDNDDLRRGRETIHKKW
                     NANVAILTGDLLHALAFKSLNGSPESVLPRVLDIFSDAYIGLVEGQALDKEFEKRSDI
                     KLPDYLKMIELKTAGLFAAAAQIGAVVGKADSKKEQALRDYGYYCGMAFQIQDDLLDV
                     IGDEATLGKDICSDLMEGKKSYIALHCVQDKYGKDFLSVFQNVTDPDILKRVIEDFRK
                     FIQKTGIQEKTEKAIAKYVQSAVQSLDRIKDSSAKAQLVAIAHRAGQRKN"
     assembly_gap    1131..1230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1275..1799
                     /locus_tag="PK937_12230"
     CDS             1275..1799
                     /locus_tag="PK937_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNH33385.1"
                     /translation="MPNHVFIAASLDGFIAKTNGSLDWLDRIENPDHDDFGYDEFLKK
                     IDAIVMGRSTYDFVKNITPWPYKKPVYVLSRSMSPQTLPNQITITDRSPLELQQELNR
                     MGYRNLYVDGGKTIQSFMRENLIDEWTITRIPIMLGAGIPLFDNTLEKETSLVHIATR
                     VFSNGLVQSTYKKQ"
     gene            1856..2344
                     /locus_tag="PK937_12235"
     CDS             1856..2344
                     /locus_tag="PK937_12235"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH33386.1"
                     /translation="MNPHLQGIVGWYGHESQSSVRMISAIPDNKLNQKIHDKFRTAGE
                     LAMHLGDTVRDLTATFKTGAVVFTPVITATPPKTADAVKHYNDAMSEFMEASKNFTDA
                     QLNKNYPFEMGGQVVWNPTGMELISGYICHEIHHRAQIGVVLRLVGAKVPGMYGPTAD
                     DM"
     gene            2361..3110
                     /locus_tag="PK937_12240"
     CDS             2361..3110
                     /locus_tag="PK937_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNH33387.1"
                     /translation="MIDVTQHDGIAILQMQHGKANALDIEFCHALAHAVDNLRHVEVH
                     GLILTGTGTMFSAGVDLPRLIKSGPEYIPEFLAALHHTFETLFTFPKPMIAAINGHAI
                     AGGCVLAACADYRIMAGGRIGVSELRVGVPFPVSALEIMRSVVPQKFLSEVLYDAQNY
                     TPDDAKTVGLIDAITGPDELMSEAVKKVKSWSAIPATAFAITKRQLQAPYLDRIHHNA
                     IDTYVVNLWKEPATLNAIGNFVASTIKKTQP"
     gene            complement(3236..4897)
                     /locus_tag="PK937_12245"
     CDS             complement(3236..4897)
                     /locus_tag="PK937_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33388.1"
                     /translation="MRTKLLLLASAFLFLSGCAQLGELAGNLMTQSTTDLQACGTTVY
                     YISNLYPAAVKSTETSYMGEAWKEGRSAVTVMNFKKHGIGMYKIDGIVAMDGTPMSHV
                     AFGAYTKLVDSGDTSPKKITIETKTGQKAEFTIKPTYPVRIKSINGKADGAEIDVTQD
                     LVLEMENPKGSERTMYKVGMLMDLMGTTSFVDVVVCRSADKIVIPHQAFVNAQVVASS
                     DQAIGTSIASFREGASWLRVERYEHDLSPNTGTGGYQGISASWDTKPVTVKGKSPSKS
                     NVQITGEEKGFSFTANKPNAYYGRALSMGKKFALASFSVRGTIYHRDVKSSTSTQTMG
                     NVEVTTTTTTITTLEFPQLPDEFWDKLLDNMYTDFVTALKNEHQIELIPVESVTKASG
                     YAAMEPIEDKNTQVKIVRSYKNTKNLLPTSLTSILKSVSSTFASDRPDARLMRELGVD
                     GLISATLDLQISTDENNHVVLNPVFTYQLLGPPNGYLVGTQYVTGTVLGKGAPFNAEE
                     VKSNPNSLNGVVRKEDMMAAFRKSIIELKKLEEAEGYTKIWNLQK"
     gene            5310..8189
                     /locus_tag="PK937_12250"
     CDS             5310..8189
                     /locus_tag="PK937_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33389.1"
                     /translation="MKSTIVFVLFLSSVLYAQRTGCVSGNCENGEGTYVWSSGTTYAG
                     EWKNGKQHGKGKTIWSDGEWYEGGYNMGERTGWGEFHWKDKSWYRGGYSNSRMHGFGE
                     YHYANGATYTGQMRLGKFSGVGTKLKNDGAREIGFWKNDTYMGSQRPVLVVSSQGPIR
                     SIQAAIDSATSGDEILIRRGTYNEAIVIDGKENITIRGEGEVWLKVPTGDIISIRNSS
                     GIALFNLKGIHDTRDEYFSCETSDVLDIVFSKNIIVAQCLLDGSGQIGLTGYHVEGIL
                     VSHTIVQNCIFGGITLENCTDVTLQYNLIIAQKAAVDKSMDCIGLSLVSCKNAVVNHN
                     TVAKLIDFNPVWIRDSESILFTNNIISHNTLNAENQSGLMLGENQTLDYHRNLLWDNK
                     TRDGKEINYDAYAKSFKPLDDINTDPLFLNPQSKQFGLKTSSGALNQATDGLDIGAVQ
                     TKHRGNLTPKITDQTPPLIVWSGQNFTDKQNIPLSDNMIKFSGRLTDNTELAGMVIGN
                     KTVSLKTDGAFNAEIVLEDDMGMIPVRAWDIFGNAAYLDIFYQKSSTPTASVQTGTVP
                     QTITANRPTTSSSAVKSSSATPNTSTVTVTSDCKTSDECIERSFKAQSIEEAILYAES
                     AVRLLKPTDEEVTQKMAYRIRAETYLYQFQKSNDAALVGKIEADFEKAIKLDPQDGVP
                     YIGLAKLYETQNQELMVEDQFARGLQANASPALLYYERSQYYIRQKKESAADEDLDKA
                     IEMLGGTSYKDGTYKITAGDPSVTDKGKSNMYYTRAERRMAREDYKSAYDDFTMALKY
                     NPQSSNAYWNRAKIIIHIDPYFKKPEPALADLNKVIELSPDFADAYYWKGQALLANSQ
                     TYTDHTATIETLVKAAELDATFSDGYAYASQLCLVNERHTEAERFAFLAVQKTDKKNA
                     LGLAHYVLGYTYSYKRDRENMLNHFRKSVEYGYEESRNVLKSMGY"
     gene            8210..11737
                     /locus_tag="PK937_12255"
     CDS             8210..11737
                     /locus_tag="PK937_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH33390.1"
                     /translation="MHVQRTILIVLGFLMIPFSLTAQTPQVELQLQPPDGVGQMVFSP
                     DGKWLAMGASDIRLLHIPSQKIINLGYHKNRVLCLAISPNGRYLASGDGKGDIYVWDM
                     LQSKMASVLHDTTGSQKILFSEDGANIIACQSFDVHIWNLAQRKKIKSYPFFDAQEKI
                     RLFPNSDCSVFDYHGISPDGKWAAIAGNTSCLVSRTTEGGYKLPGFDFNQFFQAINLE
                     TGEILEKIRIPKDMDLGAVALGRQKLALVVSTDKTTPATYKALIYNLETQSIYRQINA
                     DTNRIVHLALSPDESKLVGVSAFINALQWAQGSVLMWDIPTSRLIGTNKMAYHKTVFS
                     ADGYWLAGGFDTYLLAAPVKNFPRGVYTMGPEYSANVYDFVLDEDRGKILFSDREHVM
                     TWDIKRGVLSQKFVGSGDQTIRGVAFTAKGILTAGFRGSIVHWDSSGKNQIARMQHPV
                     LNLKNITRIDDSTYASYGTGIPSLNQDTLRFWNVYNGALTRIQTAHSDKINGMRRSPD
                     EKIWVTFGGEEKNSPSGSVCVWDATQWTKTGDYHISDQTTEVIQVLIYPDNKTALIAT
                     DHAERIVMNLHDGSIIRRMNDSGNYAVTMEFSPDGALYACGNGGPWLLGSSDYHVRIY
                     DAESHRLVKILKGHTNKIEKLQFTKDGRTLVTGSLDGTIKFWNLSNQEVTSLIGVSED
                     TYVLFTENGYYMTSKAGRDLVAFRSGMKLMTFDQFDLAYNRPDVILARLGHTPPEQLN
                     LYKQAYEKRRLRNEGVVAAALIRDDAPDIDVTTDTPPETNTSAMPLKVKIRDAKNNLK
                     TLKVMVNDVPVYGRAGLDLRGRNAQNYETDMAITLSEGKNRIQISAINTAGVESIPET
                     FETTFSGWPEPTDVICVSVGIANYQDPDYNLTYSRKDAEEMAAAFETMQKNQRGIFEK
                     IILTDTSAVKENILAIKKRLMQTRPNDRVILFFAGHGLLDEKLDWYFATYDTDFRNPS
                     KRGLGYSEIENLLDGIPARRRLVLMDACHSGEVDKETSAPVMTLVDGGPSSVSARGLK
                     QKIGIGENPVQNVNSRGFLAKKTGAVGLQNSFDLMRELFADLQNGIGATVISSASGTE
                     LALEGGEWKNGVFTYSVLQGLTNGKADLDHDGIIRCSELQTYVTQSVKQLTQGKQTPT
                     ARKEKMLDNFEVVKFTGPIDPSLWRKPGY"
     gene            11814..>12508
                     /locus_tag="PK937_12260"
     CDS             11814..>12508
                     /locus_tag="PK937_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH33391.1"
                     /translation="MSDNKIIFSMVGVGKVHKPNKQVLKDIYLSFFYGAKIGVLGLNG
                     AGKSTLLRIIAGTDKDYLGEITAQKGITFGYLPQEPELDPNKTVKEIVQEAVADTVAL
                     VREYEKIAEQMADPNADFDTLLEKQGKLQEEIEHKNAWDLDSRLEMAMDALRCPESDT
                     PVKVLSGGERRRVALCRLLLQEPDVLLLDEPTNHLDAESVYWLEQHLAQYKGTVIAVT
                     HDRYFLDNVAGWIL"
BASE COUNT         3532 a         2828 c         2805 g         3243 t
ORIGIN      
        1 tgcatggacg ccggctttat tgataacggt gatttccctt tcgatcatgg attcggcggt
       61 agccctcggt gtgaagcgtg tatcagtttt tacgttgtcc ggcgcgatgt gcgatagcta
      121 ccaattgggc tttggcgctg ctatctttga tgcggtccag actttgaacg gccgattgga
      181 catacttggc aatcgccttt tccgtttttt cctgaatacc cgttttttga atgaattttc
      241 taaaatcttc gataacacgt ttgagaatat cggggtcggt gacattctga aaaacggaaa
      301 gaaaatcttt gccgtattta tcctgtacac aatgaagcgc gatgtacgat ttttttccct
      361 ccataagatc gctgcatata tctttaccca gcgtcgcttc atccccgatc acatccagta
      421 aatcgtcctg aatctgaaac gccataccgc aataataacc gtaatcacgt aaagcttgct
      481 cttttttgga gtccgctttt ccgactacag caccgatctg ggcggcggcg gcaaataaac
      541 cggcggtttt cagttcgatc attttaagat aatcagggag ctttatatcc gaacgttttt
      601 caaattcttt atccaatgct tggccttcga ccaatccgat ataggcatcc gaaaaaatat
      661 ccaatacacg aggtagtacg gattcaggag aaccatttaa tgatttgaaa gccaaagcat
      721 gcaataagtc accggtgaga atagcgacgt tcgcattcca ttttttatgt atggtttccc
      781 gtccccgccg caagtcatca ttgtccataa tatcgtcatg gaccagtgaa aaattatgca
      841 aaagctccac agccgctgcg gcagggaatg attttttata atttttttca aacaatgcgc
      901 aggacaatac ggtcatcact gcacgcattc gcttgccgcc gctttgaaaa agataacgcg
      961 ccggatcgta aagagtagtc ggattacgtt tagctaaata aactttgatc gctttgtcaa
     1021 tttctttgac aatgggatct gtctttagag gtgcatgttt cacggttgga gtcctgaaga
     1081 cgtgaacaga ctttcagaaa atctaaatac taaattaaga gaaatcaagc nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttaattcct gactactttc caaattacgg
     1261 aatacgttta ttccatgcct aatcatgttt tcatcgcggc aagccttgac gggtttattg
     1321 ccaaaaccaa cgggtctcta gattggcttg atcgtatcga aaatccggat catgatgatt
     1381 tcggttacga tgaatttttg aaaaagatag atgcgatcgt gatggggcgg agcacctatg
     1441 actttgtaaa aaacattaca ccttggccct acaaaaaacc ggtatacgtt ctaagccgga
     1501 gtatgtctcc acaaacactt ccgaatcaaa ttacgatcac tgatcgctcg ccgctcgagt
     1561 tgcagcagga attaaaccgg atgggctatc gcaatctata tgtggacggc ggtaaaacga
     1621 ttcaaagttt tatgcgggaa aatctcattg acgaatggac tatcacgcga attcccatta
     1681 tgctcggcgc cggcataccg ctttttgaca acacacttga aaaagaaacc tctcttgttc
     1741 acattgcaac acgtgttttt tccaatggtc ttgtacaaag cacgtataaa aaacaataat
     1801 gatattagat gtacttatcc ctcatatttc ttaatttaaa aaaaaggagt cggttatgaa
     1861 tccgcatttg caaggtattg tcggttggta tggtcacgaa tctcaatctt ccgtaagaat
     1921 gatcagcgcg atacccgaca ataaacttaa tcagaaaatt cacgataagt ttcgcacggc
     1981 cggcgaactg gccatgcatc tcggcgacac ggtacgtgat cttaccgcta ccttcaaaac
     2041 cggcgctgtt gtgtttacac ccgtcattac cgcgacgcct cctaaaaccg ccgatgccgt
     2101 caaacactac aatgacgcga tgagcgaatt tatggaagcg agcaaaaact ttacggatgc
     2161 acaactgaat aaaaattacc cttttgaaat gggcggccaa gtggtttgga atcccacggg
     2221 tatggaattg atttccggtt atatttgtca cgaaatacat caccgtgcac agatcggcgt
     2281 cgtactgcgt ttagtcggcg ctaaagtgcc cggtatgtac ggaccgacag cggacgatat
     2341 gtaaaaaagg tgatacggat atgatcgatg ttacgcaaca cgacggaatc gccattctgc
     2401 aaatgcagca cggcaaagcc aatgcgctgg atatcgaatt ttgccatgcg cttgcacacg
     2461 cggtggataa cctgcggcat gtggaggtgc atggccttat cctcaccgga acaggcacga
     2521 tgttttctgc gggtgtcgat ttacctcgcc taatcaaatc cgggccggag tatatccccg
     2581 agtttcttgc ggcgcttcat cacaccttcg aaacactttt tacttttcca aaaccgatga
     2641 tcgccgctat caatggtcac gccattgccg gtggctgcgt attggccgcc tgtgcggatt
     2701 atcgcatcat ggccggaggt cgtatcggcg tttcggaatt gcgcgtcggg gttccatttc
     2761 cggtatcggc tctggagatc atgcgatccg ttgtacctca aaaatttttg agcgaagtat
     2821 tgtacgacgc ccaaaactat actccggatg acgcaaaaac cgtcggcttg atagatgcga
     2881 tcaccggtcc tgacgaactc atgtccgaag ccgtcaaaaa agtaaaatcc tggtcggcca
     2941 ttccggctac cgcttttgca attactaaac gacaactaca agccccgtac cttgatcgca
     3001 ttcaccacaa tgcgattgat acttatgtcg taaatctctg gaaagaaccg gccacgctta
     3061 acgcaatcgg taacttcgta gcaagcacca taaagaaaac ccaaccttaa aatatcttac
     3121 aaatagacct caaaacagtt ttctttttca gctgtgattt cattttatat cacgacttgt
     3181 tttaaagaaa aactattttg aggtctattt ttttatcggt taaaccgctg caaatttatt
     3241 tttgtaaatt ccaaattttg gtataaccct ccgcttcttc cagttttttt aattcgatga
     3301 tggatttgcg aaaagcggcc atcatatctt ctttgcgcac taccccattg agactattgg
     3361 gattggattt gacttcttcg gcattaaacg gggcaccttt gccgagcacc gtaccggtaa
     3421 catattgtgt gccgacgaga taaccattgg gcggtccgag caactgatac gtaaaaaccg
     3481 ggttaagtac gacgtgattg ttttcatctg tcgaaatctg caaatccaac gtcgcactga
     3541 tcaaaccatc cacccccaat tcgcgcatca gacgtgcatc cggccggtcg ctggcaaatg
     3601 tggatgaaac cgacttgagt atcgaagtta gcgacgtagg aagaaggttt ttggtatttt
     3661 tataactgcg aactattttg acctgcgtgt ttttatcttc gatcggctcc atcgctgcat
     3721 agccggaggc tttcgtaacg ctttcgacgg ggatcaactc gatctgatgt tcgtttttca
     3781 gcgccgtgac gaaatcagta tacatattat ccagcagttt atcccaaaat tcgtccggca
     3841 actgcggaaa ttccaaagtc gtaattgtcg ttgttgttgt cgtgacttcg acattaccca
     3901 tcgtttgcgt tgaagtagag gatttcacat cacgatgata aatggttccg cgtacagaaa
     3961 aggatgctaa ggcgaatttt ttgcccatgg ataaagcgcg cccgtaatag gcattgggtt
     4021 tattggcggt gaaagaaaag cccttctctt cgccggtaat ctgaacattg cttttgcttg
     4081 gcgatttgcc tttgaccgtt accggctttg tatcccagct cgcggatatc ccctgatatc
     4141 caccggtacc ggtattgggt gacaaatcat gttcgtaacg ttcgacacga agccaactgg
     4201 cgccttccct aaaactcgca atggatgtac cgatcgcttg atctgaactg gccacgactt
     4261 gtgcgttgac aaaagcctgg tgcggtatga cgattttgtc tgctgaacgg caaaccacca
     4321 catctacaaa cgacgtcgta cccatcaagt ccatcagcat gccgacttta tacatcgtac
     4381 gttcggaacc tttgggattt tccatttcca gaacaagatc ctgcgtaacg tctatttcag
     4441 cgccatcggc tttaccgtta atagatttga tgcgaacggg atatgtaggt tttatcgtaa
     4501 attcggcttt ttgacccgtt ttcgtttcga tcgttatttt tttgggtgag gtatcgccac
     4561 tatccacaag tttggtatat gcaccaaaag ccacgtgcga catcggtgtg ccatccatag
     4621 ccacgatacc gtctatttta tacatcccga taccatgttt tttaaaattc atcaccgtta
     4681 ctgccgagcg gccttctttc catgcttcgc ccatatagga agtttccgtg cttttgacgg
     4741 cagcaggata aagattgcta atataataca ccgtcgtacc acaggcttgc aaatccgtcg
     4801 tggactgcgt catcaggtta ccggcaagtt cgccgagttg agcacagccg ctcagaaaca
     4861 aaaatgctga agcgagaagt agaagttttg ttctcataga tgtaatcctg tggtttgatg
     4921 gttatatgtc gaattaagat taaacatgac ggtacagata actattagat agaagcaatt
     4981 agcataccat aatgaagcgc tttataactc attgatgttt ttacaaatga aaaaagaata
     5041 acttagttgg cgttacataa acgcgactac atagaaaaat agtctgtcgc ctcaagccga
     5101 cactcataaa aaatcccgat ttacataaaa actcgctctc caaaaagccg ttttgtatat
     5161 agaagtgaat ttgaaatttt aaaaatatgt ctttcaaaaa ggcgattcat ctattacttt
     5221 taaacacctc acgcactgat tatagttgac tcccgattct cttctttgca tttcctgata
     5281 cccaatatat cgtattgaaa ggacatcgta tgaaatccac aatcgtattt gtgctattct
     5341 tgtcttccgt tttgtatgct caacgtaccg gttgtgtgtc gggcaactgt gaaaacggcg
     5401 aaggcacata cgtatggagt tccggcacta cgtatgcagg tgaatggaaa aacggcaaac
     5461 aacacggaaa gggtaaaaca atctggagcg atggcgagtg gtacgaagga ggatacaata
     5521 tgggagaacg tacaggatgg ggagagtttc actggaaaga caagagttgg tatcgcggtg
     5581 gatattcaaa tagtcggatg catggcttcg gtgaatatca ttacgcaaac ggagccacct
     5641 atacggggca aatgcgttta ggaaaattta gcggcgttgg cacaaaattg aaaaacgatg
     5701 gcgcccgcga aatcggattt tggaagaacg atacttatat gggttcacaa cgtcccgttt
     5761 tggtagtatc atcacaaggc cccattagaa gcattcaagc cgcgattgac tccgcaacct
     5821 ccggggatga aatccttata cgacgcggca catataatga ggctatagta atcgacggaa
     5881 aagaaaacat cactattcgc ggtgaaggcg aagtatggtt gaaggttcca accggtgaca
     5941 ttatttctat tcgaaactcc tcaggcatcg cgttgttcaa cctcaaaggc attcacgata
     6001 cgcgcgatga atatttttcg tgcgaaactt cggatgtgtt ggatattgtt ttttccaaaa
     6061 acatcatcgt tgcgcaatgt ttattggacg gatccggaca gataggactc acgggatacc
     6121 atgttgaagg aatattggtg agtcatacga tcgtacaaaa ttgtattttc ggaggtataa
     6181 ccctcgaaaa ctgcaccgat gtaacactgc aatataatct gatcatcgca caaaaagccg
     6241 ccgttgataa gagcatggat tgtatcggtt tatcgcttgt ctcatgtaaa aacgccgtcg
     6301 tcaaccacaa tacggtcgcc aagcttattg attttaatcc ggtatggatt cgcgattcgg
     6361 aatctatact tttcaccaat aacatcataa gccacaatac acttaacgcc gaaaatcaga
     6421 gcggtttgat gctcggtgaa aatcaaactt tagattatca ccgaaatctc ctttgggata
     6481 ataaaacccg ggacggcaag gagattaatt atgatgcgta tgctaaatcg tttaaacccc
     6541 tagatgacat aaataccgac cccttatttt taaatcctca atccaaacaa tttggtctca
     6601 agacttcatc gggtgcgcta aatcaagcaa cggacggact ggatatcggc gctgtccaaa
     6661 ccaaacatcg cggcaatctg acgcctaaaa taaccgatca aaccccaccg ctaatagtat
     6721 ggtctgggca aaattttact gataaacaaa acatcccatt gtcagacaat atgataaaat
     6781 tctccggacg actgacggat aacacggaat tagcaggaat ggtcattggc aataaaactg
     6841 tatctttaaa aacagacggg gcttttaatg cagaaatcgt tttagaagat gacatgggca
     6901 tgatacccgt acgcgcatgg gatatcttcg gaaacgcggc ctaccttgat atattttatc
     6961 aaaaatcatc aacacctacg gcatccgtcc aaaccggaac cgtgcctcaa acgataaccg
     7021 ctaaccggcc tacaacttct tcatcggcgg taaaatcttc ttccgcgaca cccaatactt
     7081 cgaccgtaac tgtaacctcc gattgcaaaa cttcggacga atgcattgag cggtcgttca
     7141 aagcgcaatc catcgaagaa gcgattttat acgccgaaag cgccgtacgc cttttaaagc
     7201 cgaccgatga agaagtaact caaaaaatgg cctatcgtat tcgtgccgaa acatacttgt
     7261 atcaattcca aaaaagcaac gatgcggcgc ttgttggaaa aatcgaagcg gattttgaaa
     7321 aggccatcaa gttagaccct caggacggtg tgccgtatat aggactcgct aaactttatg
     7381 aaacacaaaa tcaagaattg atggttgaag atcaatttgc gcgcgggttg caagccaacg
     7441 ccagtcctgc gctattgtat tatgaacgtt cccaatatta tatacgacaa aaaaaggaat
     7501 cggcggctga tgaagactta gacaaagcca tcgaaatgtt gggcggaacc tcatataaag
     7561 acggtacata taagataacc gccggcgatc cttccgtcac cgacaaggga aaatctaaca
     7621 tgtattatac ccgcgccgaa cgacggatgg ctcgggagga ctacaaaagt gcgtatgatg
     7681 attttacgat ggcgctcaaa tataatccgc aaagcagtaa tgcttattgg aatcgcgcca
     7741 aaatcatcat tcacatagac ccctatttca aaaaaccgga acctgcactt gccgacctca
     7801 acaaggtgat cgagctctca ccggattttg ctgatgctta ttattggaaa ggccaggcgt
     7861 tgttagccaa ttcgcaaacc tatacggatc ataccgcgac catagaaacc cttgtcaaag
     7921 cggcggagct ggatgcgacg ttttctgacg gatacgccta tgcttcgcag ctctgtcttg
     7981 tcaatgagcg tcacaccgaa gccgagcggt ttgctttttt ggccgtacaa aagaccgata
     8041 aaaaaaatgc attgggtctt gcgcattacg ttttgggtta tacctattcg tataaacgtg
     8101 accgcgaaaa catgctcaac catttcagaa agtctgtcga atacggttat gaagaatcaa
     8161 ggaatgttct aaaaagtatg ggatattaaa ggcaaacggg agcaaagcca tgcacgtaca
     8221 gcgtacgatt ctaattgtgt tggggttttt gatgataccg ttttctttaa cggcccagac
     8281 gccccaggtg gagttacaat tacaaccacc ggatggcgta ggacaaatgg tcttcagtcc
     8341 tgacggtaag tggcttgcta tgggcgcttc ggatatacgt cttctgcata taccatctca
     8401 aaaaatcatc aatctcggtt atcataagaa ccgcgtattg tgccttgcga tcagccctaa
     8461 tggtcgctat ctggcgtccg gagacggcaa aggcgatatc tacgtatggg atatgctgca
     8521 atccaagatg gcatcggttc tccatgatac gacaggatca caaaagattc ttttttcaga
     8581 ggatggagcc aatattatcg catgccagag ttttgatgtg catatatgga atcttgccca
     8641 acgaaaaaaa ataaaatcct atcctttttt tgatgcgcag gaaaaaattc gtttgtttcc
     8701 aaacagcgat tgttcggtat ttgactatca cggcatcagc ccggatggca agtgggcggc
     8761 gatcgccggg aataccagtt gtctggtttc gcgcactaca gaaggcgggt ataaattacc
     8821 cggttttgat tttaatcaat ttttccaggc gatcaatctc gaaacaggcg aaatacttga
     8881 gaaaattcgt attccgaaag acatggatct gggggctgtg gcgctgggtc gtcaaaaatt
     8941 agcgctggtc gtatccaccg ataaaaccac acctgcgaca tataaggcgc tgatatacaa
     9001 tcttgaaacg cagagtattt accgtcaaat caacgccgat accaatcgta tcgtacacct
     9061 tgcactctct ccggacgaat ccaaactcgt cggagtttca gcctttatca atgcattgca
     9121 atgggcgcag ggttcggtac taatgtggga tattccgact tctcggctta tcggaacgaa
     9181 taaaatggca taccataaaa cggtgttctc cgcggatgga tattggttgg ccggtggttt
     9241 tgatacatac ctgctcgctg cgccggtcaa aaattttccg cgcggcgtat atacaatggg
     9301 acccgaatat tctgctaatg tgtacgattt tgtcctcgat gaagatcggg gcaagatact
     9361 cttttcggat cgcgaacacg tcatgacatg ggacatcaaa cggggtgtac tgagtcaaaa
     9421 atttgtaggg agcggtgatc agacgatacg cggcgtagcg tttacagcca aaggtattct
     9481 tacggccggg tttcgcggtt cgatcgtgca ttgggattcc tccggcaaaa accagatcgc
     9541 acgtatgcag catccggtac tgaatttgaa aaacattacg cgtattgatg actcgaccta
     9601 cgcatcatat ggaaccggga taccgagtct taatcaggat accttgcgtt tttggaatgt
     9661 gtataacggc gcgttaacac gcatccaaac agcgcattcc gataaaatca acggtatgcg
     9721 caggtcaccg gatgagaaga tatgggtaac tttcggcggg gaggaaaaaa actctccgag
     9781 cggctccgtc tgcgtgtggg acgcaaccca atggaccaaa accggcgatt atcatatcag
     9841 cgatcaaacg accgaagtaa ttcaggtgtt gatctatccg gacaataaaa cggcattgat
     9901 cgctacggat catgccgaac gtatagtgat gaacctccat gacggctcca taattcggcg
     9961 tatgaatgat agcggaaact atgctgttac tatggaattt tctccggacg gcgccttgta
    10021 tgcctgtggt aacgggggac catggcttct gggttcgtcg gattatcacg tacgcatcta
    10081 tgatgcagaa tcccatcggt tggtgaaaat attaaaaggt cataccaaca aaattgaaaa
    10141 acttcaattc acgaaagacg ggcgcactct tgtaaccggg agtttggacg gtaccatcaa
    10201 attctggaat ttgtccaatc aggaagtgac ctcgctcatc ggcgtatcag aagacacgta
    10261 tgtcctcttt actgagaacg gatactatat gacatccaaa gccggacgcg atttagtggc
    10321 gtttcgcagc gggatgaagc tgatgacctt tgatcaattt gatctggcgt ataaccgtcc
    10381 cgatgtgatt ttagcacgat tgggtcacac tccgcccgaa cagttgaact tatacaagca
    10441 agcctacgaa aagcgacgct tacgaaatga aggcgtcgta gcggcagcgc tgatacgcga
    10501 cgacgctccg gacatagatg tgacgacaga tacgccgcct gaaaccaata cctccgcaat
    10561 gccgttgaaa gtaaaaatcc gtgacgctaa aaataatttg aaaacactaa aagtcatggt
    10621 caatgatgtg cccgtatatg ggcgcgcagg tctggactta cgcggtcgaa atgcacaaaa
    10681 ttatgaaacc gacatggcga tcacgctcag tgaaggaaaa aacagaattc agatttccgc
    10741 tatcaatacc gccggcgtcg aatccattcc cgaaactttt gaaaccactt tttccggatg
    10801 gccggaaccc accgatgtga tatgcgtatc ggtcggaatt gccaactatc aggacccgga
    10861 ctacaatctt acatactcac ggaaagatgc ggaagaaatg gcggcggctt ttgaaacgat
    10921 gcaaaaaaat cagcgcggca tttttgaaaa aatcatactt acagatacct ccgccgtcaa
    10981 agaaaacatt ctcgccataa aaaagcgtct gatgcaaacc cgtcccaacg atcgtgtgat
    11041 tttgtttttc gcaggtcacg gattgctgga tgaaaaactc gattggtatt ttgcaactta
    11101 cgataccgat ttcagaaatc catctaaacg tggtttagga tattcggaaa ttgaaaattt
    11161 gctcgatggt attcccgcac ggcgacgatt ggttttgatg gatgcatgcc attccggtga
    11221 agtggataaa gaaacatcgg cgccggttat gacactagta gacggcggac catcttccgt
    11281 ctcagcgcgc ggtcttaaac aaaaaatcgg catcggtgaa aacccggtgc aaaacgtaaa
    11341 ttccagaggt tttttggcca aaaaaaccgg ggcggtggga ttgcaaaact cgtttgatct
    11401 gatgcgtgaa ctttttgccg acctgcaaaa cggcattggc gctaccgtga tcagctccgc
    11461 ttcaggaacc gaattggctc ttgaaggcgg cgaatggaaa aacggcgttt ttacatacag
    11521 cgttctgcaa gggcttacca acggtaaagc tgatctggat cacgacggca tcatacggtg
    11581 ttcggaactt caaacctacg taacccagtc cgtgaaacaa ctgactcaag gaaaacaaac
    11641 gcccaccgcc cgtaaagaaa aaatgttgga taattttgaa gtggtaaaat ttaccggacc
    11701 gatagatcca tcgctatggc gaaagcccgg gtattgattt tctgcgttct aagtgtaact
    11761 taggggcgat ttaaaaaaca cggtatttat tttatcaatt ttaacgagcg gatatgagcg
    11821 acaataagat tattttttcg atggtcggcg tgggcaaagt gcacaagccc aacaaacaag
    11881 tgctcaaaga tatttatctg tccttttttt acggcgccaa aatcggcgta ctcggtctca
    11941 acggcgcagg taaatctacg ctgcttcgta tcatcgcagg aaccgacaag gattacctcg
    12001 gtgaaatcac agcgcagaaa ggcatcacct tcggttatct gccacaggaa ccggaactcg
    12061 atccgaataa aacagtcaaa gagattgttc aggaagcggt tgccgacacg gtagctttgg
    12121 tacgtgaata cgaaaaaata gccgaacaaa tggccgatcc taatgcggat tttgatacgc
    12181 ttttagaaaa acaaggcaaa ctgcaggaag agatcgaaca taaaaacgca tgggatcttg
    12241 acagccgtct tgaaatggct atggatgcac tgcgttgccc ggaaagcgac acacccgtca
    12301 aagtactttc cggcggtgaa cgccgtcgcg tcgctttgtg ccgtctgctt ttgcaggaac
    12361 ctgacgtttt attattggat gaaccgacca accatcttga cgcggagtcc gtgtattggc
    12421 tggaacaaca tttggctcaa tacaaaggca ccgtcatcgc cgtcacccac gatcgttatt
    12481 tcctcgataa tgtcgccggt tggatact
//
LOCUS       DAOQGF010000100        12337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2385_length_12337_cov_7.583700, whole genome shotgun sequence.
ACCESSION   DAOQGF010000100 DAOQGF010000000
VERSION     DAOQGF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_12337_cov_7.583700"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2594
                     /locus_tag="PK937_12265"
     CDS             <1..2594
                     /locus_tag="PK937_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH33392.1"
                     /translation="NASDYSDEVSSSPYDQTAPAVPQDLAATAGDGQVTLNWMANGES
                     DLARYRIFINDVQSDSTSDTSYTATALTNYTLYSFKIAAVDTSGNFSGFVSSTSIPYD
                     QTAPSEVTGLSVTRGDGALTLNWTANGESDFKAYRLFMGTSPNPTTQVDSVTTIATTS
                     KEFGGLVNGTTYYYRMMAVDTSGNASDYSDEVSSSPYDQTAPSVPQNLTATAGDGQVT
                     LNWTANAENDLARYRVYMNGTQIDSTTNNSYTKNDLTNYTSYDFSISAVDTGKNVSAQ
                     TTTENATPYDQTAPAVPQDLAATAGDGQVTLNWTANGEGDFAHYRVYMDGTQIDSTTN
                     NSYTKTGLSNYTTYNFKLSAVDTSRNASALTSQVSSMPYDQTAPAVPQDLAATAGDGQ
                     VTLNWTANSESDLARYRVYMDGTQIDSTTNNSYTKNGLTNDVTYNFKIAAVDTGLNAS
                     AFSTQVSGTPTDMTAPSKPNVLAAVAGNTSIILTWRKNIESDVIRYRIYRGTTAAPTT
                     QVDSTTSASDTTKTYSGLTNGTTYFFRVTAVDGHGNFSVYSDDISAKPLDAPGTLTVS
                     LFQNKAAKKYAQITVISSKALSAAPTVTAKLGVNNTPVTMTAIASSNQQFRGGFVINS
                     AGSYTLQTSAQTLNGRDTTVQRTFNAVIAKSGEAATLASTDGKASLKLNRATLAEEAV
                     LLSEQSDDGVYTFSADAEANGSMVLEIQFDAAQYADPSKLFIYRMEKETWSKQKTQVF
                     TKTNTLKAQVESFGTYKIAYDGSFDGSNIVPTEFALLQNYPNPFNPSTTIRYDLREDE
                     TVSLKIYNMLGQEVRTLRKGFQMAGEYRVQWDGRNNAGQVVSSGVYIYRLETKSYRAT
                     KKMMFLK"
     gene            2613..3233
                     /locus_tag="PK937_12270"
     CDS             2613..3233
                     /locus_tag="PK937_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33393.1"
                     /translation="MKRFILKNLTLVTVIASLTACGGGGKGKSAYTKLVDQGESQFAT
                     GSFADARTSFNAAKLKNPNSATAYIGAAWCSMSLNDIAAALADFQSGLSKTNINADLY
                     AGYAFALNANGDFALSNQRIDEALNADTDWELSYDGSLNVNDLRLAKAENHFLLGQYT
                     ESLAQVKLLNTSFNADVNTVSGRAALSVEIERLRNALGKNIARIPA"
     gene            3350..4771
                     /locus_tag="PK937_12275"
     CDS             3350..4771
                     /locus_tag="PK937_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH33394.1"
                     /translation="MKKNQPHILLINPYIYDFAAYDLWSKPLGLLYIAAVLEENGFRV
                     SLIDALDRHDTEMLAWQGRASAKSRMYGDGYFYREIVEKPTVFSHIPRHYARYGFTPE
                     LMRKKISDLNLEQSVDSVLVTSGMTYWYKGVHEIIAMCKELVPQAEVILGGIYATLFT
                     NYAMEFSGADRVFTGESEKTVLAYLCSQSGLEVKKNYKGYDDYPYPAYHLYSKLEYVA
                     MMTSRGCPYSCTFCATHAFTEKFTPRSPEGALHEIINYANQDIQNITFYDDALFVNSD
                     KHIKRILRGVIENGKNVNFHTPNGLFARLIDAELAKLLVQSGFKTIRLSYETKNPERQ
                     KVLRKVSDKDLESALEYLEQAGFLRHDIVVYLIMGLPDQSPDEVQAGIDYIHSLGAKV
                     SLSSFSPIPGTADWEMARQKYNFPVNEPLLTNKSVYPLRHPGFTSEDFDGLKNYAVEK
                     NRSYPKDNPTLDENHVSDYNVYA"
     gene            4764..5999
                     /locus_tag="PK937_12280"
     CDS             4764..5999
                     /locus_tag="PK937_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNH33395.1"
                     /translation="MLNRVALFLFFSLISLQAQNLQKQIDKSRNNMKKIKDEIAQYEN
                     RLTSQAQKEKTEIENLNDINEKIGLLHQLLGELEQGRRLTEERIAELRSILSQTRTEL
                     QNLKGLTSQRLLQTYKHRDEDPLAYILTSDSWTQAFARMKYLKLIAEQDQRDIQSLRT
                     KKEKIETQKKLIEVELLNQQFLISEKKREKANLDQQLARRKNTLSKIRKDKRLLASLI
                     EQRKDDMANLQTIIAALEKKKAEEEALAKKRAEEAAAAAKSGKTKTAGSVKYKEPVYT
                     EKSNFAQYKGRLPYPVPGKIVVSFGNQYNAALGTTTRNTGVDIQSREGAEVHSVAKGK
                     VSLISWLRRLGNTVIIDHGEGFYTVYAHLSEIFVSVDQHVAAGQTIANLDESDEGKAV
                     LHFEIYKDREAVDPAGWLR"
     gene            6012..6962
                     /locus_tag="PK937_12285"
     CDS             6012..6962
                     /locus_tag="PK937_12285"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNH33396.1"
                     /translation="MLSKKLSFYIKIIFTIAFVGYLIFKVDPAMIRADFSSIAWDYFW
                     IGAALTLPNLLIQWLKWQYLVRMIDPDVSRRSVWRSLFAGFSIGLITPGRIGEMGKGV
                     FIDSPQRKLIIGFGVIDKILSQIALAVFGAVSLLYLTEFVFDFNRPTQLIILVFVFWV
                     VVALAVMIFRPSSIRWLIEKLKSWVRFLPWNQHITDVLSAADEFKRHHFWPSLHYALL
                     FQWIVFIQIYCFANAFHSISFWEALSAAAAAMFVKALLPITLMDIGVREGALIFFLGR
                     FGVDAASAFNTSVMIFASNVLLPGLIGVFFVYKFRWSREK"
     gene            6952..7584
                     /gene="thiE"
                     /locus_tag="PK937_12290"
     CDS             6952..7584
                     /gene="thiE"
                     /locus_tag="PK937_12290"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNH33397.1"
                     /translation="MKNNIPFRFYGITDRKRMIESLFLSHLDRAVKAGLRALQIREKD
                     LSPRALYELTDKIRTISATRSVRIFINDRVDIAMALDLDGVHLSTQSMPTYNVRKLVG
                     PDKLIGVSTHDLHEAIHAENEGADFIVIGPIGETRSKPKGHAVLSEKEFEIIREEVKI
                     PIFVLGGVQTNNISHWLQKGAHGAAGISLLMDDENLEERFSNLKSTLGSL"
     gene            7590..8195
                     /locus_tag="PK937_12295"
     CDS             7590..8195
                     /locus_tag="PK937_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNH33398.1"
                     /translation="MKKTEAIIFAAGQASRYGDNKLLLPIGDVPAMIYAIRTLCAAGV
                     DRIAVITGAYHDTIKQALIREPVRIFLNPRHSEGMSATICFSVQQIDQSSESILLLPG
                     DVPLFSRQTIKTMMQYGLTHRIVIPRYQGKKGHPVLVDRKIVEECLETGLEMPLYEVI
                     QNHQNEVFYLDSDDQGVVLDIDTREDYDAILSYFKTTYGSY"
     gene            complement(8170..8793)
                     /locus_tag="PK937_12300"
     CDS             complement(8170..8793)
                     /locus_tag="PK937_12300"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNH33399.1"
                     /translation="MLKKGYMRIILGFFCFVLSLQVSLHSQNIDARLFKAINKHHSPG
                     WDRFYEFQSRSVKPVYIATPLSFVATGLLRKDTYMRDAGILMTTSAVANLAITYSMKA
                     AINRERPYKALNNVHTVGSTERSASFPSGHTSSAFNMATSLSLAYPKWYVIVPSYAWA
                     GVVGYSRIKMGMHYPGDVLAGALIGAGTSYLTFKLQKPILNTIHKLF"
     gene            complement(8772..9170)
                     /locus_tag="PK937_12305"
     CDS             complement(8772..9170)
                     /locus_tag="PK937_12305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNH33400.1"
                     /translation="MKITRTTVRVRYAETDSMRVAYHGSYLPWMEVGRTELIRELGYS
                     YKRLESEGIFMPVIRIEVDYLRSVHYDDTVHIDAWIASQEGIRTTIAYICYRDSEPVA
                     RGFTTHAFTDRQNKPVRPPKEFIACLKKGI"
     gene            complement(9186..10148)
                     /locus_tag="PK937_12310"
     CDS             complement(9186..10148)
                     /locus_tag="PK937_12310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNH33401.1"
                     /translation="MNVPVILDFEKPIFDLEAQIADMKSAHANVDITPEIEALQNKLE
                     AMRKEIYANLTPWQKVQLARHPERPRSLDYIERISDGFMELHGDRLFGDDHAIVSGIG
                     RIDGKEVMIIGQQKGKDTKSHVYRNFGMPNPEGYRKAMRLMHMAAKFKRPILTLIDTP
                     GAYPGIEAEERGQAEAIARNLLEMSRLPVPIICVVIGEGASGGALGIGVGDKILMMEN
                     TWYSVIAPESCSSILWRTWEYKERAASELKLTADDLIRFDVVDDKIQEPVGGANRNYD
                     LAAQYLKEAVMHNFAELEKLSVEQLLDQRMEKYSKMGRWGEMSA"
     gene            complement(10207..10938)
                     /locus_tag="PK937_12315"
     CDS             complement(10207..10938)
                     /locus_tag="PK937_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNH33402.1"
                     /translation="MTRALIVVPTYNEAENIPVLIQRIFALDFSEYQVAPEILVVDDS
                     SPDGTADRVQTIQKNDTRVHLLSRPGKQGLGKAYVAGFSWALERGYEYILEMDADLSH
                     DPSYLKDMFRLIRDNDLVIGSRYINGVNVINWPLKRLLISYFGNMYARMVTGMPIMDA
                     TAGYKCFRRSVLSSMDLTKVSASGYSFQIEMNYRTWLKKFRIIETPIIFYDRTVGYSK
                     MSTNIIREGLLLVWKLRIKHFFGKI"
     gene            complement(10949..11416)
                     /locus_tag="PK937_12320"
     CDS             complement(10949..11416)
                     /locus_tag="PK937_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HNH33403.1"
                     /translation="MNLTEIAIIIHSTQESEYIKGCKETLDRFGLHCEVKVFTPLLDP
                     PELQEFMQKAPERGIKIFIATSSLQSKLAAMIAAYTHLPVIAIPIGKEGFTDRDATIA
                     SVEMPEGAPVAVVSSYPTGGKNAAILAAQILAFSDNHLMDRLKLFKQNGCRFV"
     gene            11660..>12337
                     /locus_tag="PK937_12325"
     CDS             11660..>12337
                     /locus_tag="PK937_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33404.1"
                     /translation="MKRNLLMLAFVVATASLSAGEPNVNPLMAGFNQNIEFNVLTPEH
                     IKTSTDIIIEKSKTALSGIFAIPKEKRSFDNTMLAIDNMYNDYGKVSSNIYLMANAHP
                     DKATREQALASLSVLDKFDNEIALDENLYRAVKEYSESPEAKKLQGFKKKYLEEILRD
                     FERNGFALPKEKRDQLKTMQDRLSDLGITFGRNIAEAADTLFVTEEEMKGLPDDYKSA
                     RKMSDGRY"
BASE COUNT         3570 a         2623 c         2790 g         3354 t
ORIGIN      
        1 gcaatgcatc ggattattcg gatgaagtat cttcatcgcc ctatgatcag acggcccctg
       61 cagttcctca ggacttggct gcaacggcgg gcgatggtca ggttacattg aactggatgg
      121 caaacgggga aagtgatctc gctcgctatc gtatttttat caatgacgta caaagcgatt
      181 cgacgagcga tacgtcttat acagcaactg ctttaactaa ttatacattg tattctttca
      241 aaatagcagc cgtagatacg tcaggtaatt tttcgggttt tgtgtcttcg acgtcaatac
      301 cttacgatca gacagcgcct tcagaagtaa ccggtcttag tgtaacccgt ggagacggtg
      361 cattgacact aaactggaca gcaaatggag aatccgattt taaagcctat cgtttattca
      421 tgggaacaag ccccaatcct acgacgcaag tggattcggt gacaacgatc gcaacgacga
      481 gtaaagaatt cggcggattg gtcaatggta cgacgtatta ttaccgtatg atggccgtag
      541 atacatccgg caatgcatcg gattattcgg atgaagtatc ttcatcaccc tatgaccaga
      601 cggcaccttc ggtaccacag aaccttaccg caacagccgg agatggtcaa gttacgttga
      661 actggacggc aaacgctgaa aacgacttag cacgttatcg tgtttatatg aatggtacac
      721 agattgattc gactacgaat aatagctata caaaaaacga tttaacgaac tacacaagtt
      781 atgatttttc aattagtgct gttgatacgg gtaaaaatgt aagtgcgcaa accacaacgg
      841 aaaacgcaac accttacgat cagacagcgc ctgcggttcc tcaggacttg gctgcaacgg
      901 ctggagatgg tcaggttaca ttgaactgga cagcaaacgg tgaaggtgat tttgcacatt
      961 atcgtgttta tatggatggt acacagattg attcgacgac gaacaatagt tatacaaaga
     1021 ccggtttatc gaactacacg acatataact tcaagttatc cgccgttgat accagccgca
     1081 atgcaagcgc attgacttca caagtatcat ctatgcccta tgatcagacg gcccctgcag
     1141 ttcctcagga cttggctgcg acggctggtg atggtcaggt tacattgaac tggacggcaa
     1201 acagtgaaag cgacttagca cgctatcgtg tttatatgga tggtacacag attgattcga
     1261 cgacgaacaa tagctataca aaaaacggtt taactaatga tgtaacttat aatttcaaaa
     1321 ttgctgccgt agataccggg cttaacgcta gtgccttctc cacgcaggta tcgggtacac
     1381 cgaccgatat gacagctcct tcaaaaccga atgtattagc agccgtagcc ggtaatacta
     1441 gtattatttt gacatggcgg aaaaacatcg aatcggatgt aattcgttat cgtatatacc
     1501 gtggtacgac ggccgcaccg acaacacagg tagattccac gacctctgct tcggatacga
     1561 caaagaccta cagcggtttg accaacggga cgacgtactt tttccgggtc actgctgttg
     1621 acggacatgg taatttcagt gtgtattccg atgatatttc agctaaacct ctagatgcgc
     1681 cgggtacact cacggtaagt ttattccaaa acaaagcagc taaaaaatat gctcaaatca
     1741 cggtaatatc atccaaagct ttatcagcgg ctcctaccgt gacggccaaa cttggagtaa
     1801 ataatacacc cgtcacgatg acggctatcg cgtcaagcaa ccaacagttc cgcggtggct
     1861 ttgtgataaa tagtgcaggt agctatacgt tacaaacgtc cgcacagaca ttgaatggtc
     1921 gcgatacgac cgtgcagcgc acgtttaatg cggtgattgc caaatccggt gaagcggcta
     1981 cccttgctag cacggatggc aaagcttccc tcaaacttaa tcgcgcgacg cttgctgaag
     2041 aagctgtact tctgtccgaa cagtccgacg acggtgttta tacattctca gcggatgcgg
     2101 aagccaacgg cagcatggta cttgaaatac agtttgatgc ggctcagtac gccgatccgt
     2161 cgaagttgtt catttatcga atggaaaaag aaacctggag taaacaaaaa acacaggtat
     2221 ttaccaaaac caatacgctc aaagcgcagg tagaaagttt tggaacatac aagatcgcat
     2281 atgacggttc gtttgacgga tcgaatattg tgccgacaga gtttgcgttg cttcaaaact
     2341 atccgaatcc gtttaacccc tccactacga tccgatacga tctacgtgaa gatgaaacgg
     2401 tatctctgaa aatatacaac atgctcggcc aggaagttcg tacgctgcgc aaaggattcc
     2461 aaatggccgg agagtatcgt gtacaatggg atggtcgcaa taatgccgga caagtcgtat
     2521 ccagcggcgt atatatctat cgcttggaga cgaagtccta tcgcgcaacg aaaaaaatga
     2581 tgtttttgaa ataaccgacg aggaaacaaa aaatgaaacg ctttatcctc aaaaatttga
     2641 cgttagttac cgttattgca tctctgactg catgcggtgg cggaggtaag gggaaatcgg
     2701 cttatactaa actagtcgat cagggtgaat cgcaatttgc aaccggcagt tttgccgacg
     2761 cacggacttc atttaatgcg gccaaattga aaaaccccaa ttcagcgaca gcctatatcg
     2821 gtgccgcatg gtgttcgatg tcgctcaatg atatcgccgc agcattggcc gattttcaat
     2881 ccggattatc caaaaccaat attaatgcag atctttatgc gggttatgca tttgctctca
     2941 acgcaaacgg cgattttgct ttgtccaatc aacgtattga tgaagcattg aatgccgata
     3001 ccgactggga gttgtcctat gatggcagtt tgaacgtaaa tgatttgcgt ttggcaaaag
     3061 cagaaaatca ttttctttta ggccaataca ccgaatcgct tgcacaggtc aaacttctta
     3121 acacgtcatt caacgcggat gtaaacacgg tttctggccg cgcggcgttg tctgtggaaa
     3181 tcgaacgctt gcgtaatgcc ttaggaaaaa acatcgcacg catacccgct taaaaaaatt
     3241 aaaccggatt tttataaagc cgtttcaggt ttacttgaaa cggctttttt attgtcatcg
     3301 gcaaatgcct ttgataatct taaggtgaat ggctacattg gcaatcgtaa tgaaaaaaaa
     3361 tcaacctcat attctactga tcaatccgta catttacgac tttgcggcgt atgatctttg
     3421 gtccaagccg ctggggttgc tctatatcgc cgctgttttg gaagaaaatg gttttagagt
     3481 ttctttgatt gatgcgttgg atcggcatga taccgaaatg ctggcatggc agggtcgcgc
     3541 gagtgccaaa tcccgtatgt acggcgatgg ttatttttat agagaaattg tcgaaaagcc
     3601 cacggtgttt tctcatattc cgaggcatta tgcgcgttac ggttttacac cggaactgat
     3661 gcgaaaaaaa atatccgacc taaatttaga acaatcggtc gacagtgtct tagtcacatc
     3721 cgggatgaca tattggtaca agggtgtaca tgagatcata gcaatgtgca aagaattagt
     3781 acctcaggca gaagttattt tggggggcat ttatgcaaca ttgtttacca actatgctat
     3841 ggaattttcc ggcgccgatc gtgtatttac gggagaaagt gaaaaaaccg ttttagcata
     3901 tctctgctcg cagtcgggat tggaagttaa gaaaaactat aagggttatg atgattaccc
     3961 ctatccggcg tatcatctgt actctaaact tgagtatgtt gcgatgatga catcgcgtgg
     4021 ttgtccatac agttgtacat tttgcgctac gcatgcgttt actgaaaagt ttacaccgcg
     4081 ttctccggaa ggggcgctgc atgaaatcat aaattacgcg aatcaagata tacaaaatat
     4141 tacgttttat gatgatgcgc tgtttgtaaa tagcgataaa catattaaac ggattttacg
     4201 cggtgtcata gaaaacggaa aaaatgtaaa ctttcacaca cccaatggac tttttgcccg
     4261 actgatcgac gcagagttag ctaagttgtt ggttcaaagt ggatttaaaa ccattcgcct
     4321 cagttatgaa accaaaaacc cggaaaggca aaaagtttta cgtaaagtca gcgacaaaga
     4381 tttagaatcg gctttagaat atttggaaca agccgggttt cttcgtcacg atattgtcgt
     4441 ttatttgatc atggggttac cggatcaatc tccggatgaa gtgcaagcgg gaatagacta
     4501 tatacacagt ttaggtgcta aggtaagtct ttcatcattt tcaccgattc cggggacggc
     4561 ggattgggaa atggcgcgcc aaaagtataa ctttccggtc aatgagccgc tattaaccaa
     4621 taaatcggtc taccctctgc gccaccctgg ttttacgtcc gaggattttg acggattaaa
     4681 aaactatgcc gttgaaaaaa atcgatcata cccaaaggat aatcccacgc tcgatgaaaa
     4741 ccacgtttcg gattataatg tctatgctta atcgcgtcgc actatttttg tttttttcat
     4801 tgatttctct gcaggctcaa aatcttcaga agcagattga taaaagccgc aacaacatga
     4861 aaaaaataaa agatgagatt gctcaatatg aaaaccgatt gacgtctcag gcacaaaaag
     4921 aaaaaacaga aatagaaaac ctgaatgata tcaatgaaaa aatagggttg ctgcatcagt
     4981 tattgggcga attggaacaa ggacgtcgat tgacggagga gcgtattgcc gaattgcgct
     5041 caatactttc gcaaacccgc acggaactgc aaaatctcaa aggcctcacg tcgcaacggc
     5101 ttttacaaac gtataaacat cgcgatgaag atcccttggc ttacattctt acatccgatt
     5161 cgtggacgca agcttttgca cggatgaaat atctcaaact tattgccgaa caagatcaac
     5221 gggacatcca aagtcttcgc accaaaaaag aaaaaatcga aacacaaaag aagttgatcg
     5281 aagtagagtt gcttaatcag caatttctta tttcagaaaa aaaacgcgaa aaggctaatc
     5341 tggaccagca gttggctcgc cgaaaaaaca cactgagcaa gattcgaaaa gataaacgtc
     5401 ttttggcctc gctcatcgaa caacgcaaag acgacatggc caatttgcaa acgattatcg
     5461 ctgcattgga gaagaaaaaa gccgaagaag aagctttagc aaaaaaacga gccgaagagg
     5521 cggccgctgc ggctaaatcc ggtaaaacca aaacggcggg cagcgttaaa tacaaagaac
     5581 cggtttatac cgaaaaatca aattttgctc aatataaggg acgattgccc tatcctgttc
     5641 ccggtaaaat cgtggtaagt tttggaaatc aatataatgc ggcattggga accactacgc
     5701 gtaataccgg tgttgacatt cagtcccgcg aaggagcgga agtgcatagc gtggctaaag
     5761 gaaaagtgtc gttgatttca tggttacgcc gcctcgggaa taccgttatt atagatcatg
     5821 gcgaaggatt ttatacggtt tacgcgcatc tttcggagat ttttgtgtcg gtagatcagc
     5881 atgtggccgc cgggcaaacc atagcgaatc tggacgaatc cgacgagggg aaagccgttc
     5941 ttcactttga aatctataaa gatcgggaag cggttgatcc ggcaggatgg ttgcgataga
     6001 aacgaaatat tatgttgagt aaaaaattat cattctatat caaaataatc tttacgatag
     6061 cgttcgtcgg ataccttatt ttcaaagttg atccggcgat gattcgcgca gatttttctt
     6121 cgatcgcatg ggattatttt tggattggcg ctgcattgac tctgcccaat ttgcttattc
     6181 agtggttaaa atggcaatac cttgtgcgta tgatagatcc ggatgtttct cggcgtagtg
     6241 tttggcgctc gttgtttgca ggattttcga tcggtctgat aacgcccgga cgtatcggag
     6301 aaatgggtaa aggtgttttt attgattcgc cgcaaagaaa actcattatt ggttttggtg
     6361 ttattgataa aatattatct caaatcgcgc ttgccgtttt cggagccgta tcgctcctgt
     6421 atcttacaga atttgttttt gattttaatc gtccgacgca gttgatcatt ttagtatttg
     6481 ttttttgggt tgtcgtcgca ttggctgtta tgatttttcg gccatcaagc attcgatggt
     6541 tgatcgaaaa actaaaatca tgggtacgtt ttctcccgtg gaatcaacat atcacagatg
     6601 tgctatcagc ggcggacgaa tttaaacgcc atcatttttg gccgagttta cactatgcct
     6661 tattatttca atggattgtt tttatacaaa tctactgttt tgccaatgca tttcattcga
     6721 tttccttttg ggaagcgtta tcagcggcgg ccgcggcgat gttcgtaaaa gcattattgc
     6781 ccatcactct tatggatatc ggggttcggg aaggtgcttt aatatttttt ctcggcagat
     6841 tcggagtcga tgccgcttcg gcattcaata cgtccgtaat gatttttgca agcaacgtgc
     6901 ttttaccggg gctgataggt gttttttttg tttacaaatt tcggtggtct cgtgaaaaat
     6961 aatattccat ttcgatttta cggtattacc gatcgtaaac gtatgatcga aagtcttttt
     7021 ctttcgcatc tggatcgtgc cgtcaaagca gggttgaggg cgcttcagat tcgtgaaaaa
     7081 gacttatcgc ctcgcgcatt gtacgaactt acggacaaga tacgaacaat atccgcgacg
     7141 cgcagtgttc gtatctttat caatgacaga gtagatatcg ctatggcgct tgatttggac
     7201 ggcgttcatc tttcgactca aagcatgcct acatataatg ttagaaaact ggtcggcccg
     7261 gataaactga tcggtgtatc tacacacgat ctgcacgaag ccattcacgc ggaaaatgaa
     7321 ggcgccgatt ttatcgtgat cggccccatc ggtgaaacgc gatccaagcc gaaaggccat
     7381 gcggtgctaa gcgaaaaaga atttgaaata attcgcgaag aggtcaaaat tcccatattc
     7441 gttctcggcg gcgtacaaac aaataatatc tcacattggc tacaaaaagg cgctcatgga
     7501 gcagcgggaa tttcactttt gatggatgat gaaaatttgg aagaacgttt ttcaaatttg
     7561 aaaagcacgc ttggcagttt ataaatcaaa tgaaaaaaac cgaagcgatt atttttgcag
     7621 ccggtcaggc atcacggtat ggtgataata aattactatt acccatcggc gatgtaccgg
     7681 caatgatata tgctatccgt acgttgtgtg ccgccggtgt tgatcgtatt gctgttatta
     7741 cgggggctta tcacgatacg ataaagcaag ccttaatcag agagcctgtc cggatttttt
     7801 taaaccctcg acacagcgaa gggatgagtg caacgatttg tttttcagtt caacaaatag
     7861 atcaaagttc ggaatccatt ttgctccttc ccggcgacgt acctttgttt tcacgacaaa
     7921 cgatcaaaac catgatgcaa tatggtttaa cgcatcgcat tgtaataccc cggtatcagg
     7981 gaaaaaaagg gcatcctgtt ttagtggatc gtaaaattgt ggaggaatgc ttggaaaccg
     8041 gtttagaaat gccgctttac gaagtgattc aaaatcatca aaacgaggtt ttttatttag
     8101 acagtgatga tcaaggggtt gtgttggaca ttgatacgcg ggaagactac gatgccatct
     8161 tgagttattt caaaacaact tatggatcgt attgagaatt ggcttttgta atttgaatgt
     8221 gagatacgaa gtgcctgcgc cgataagagc gccggctaat acatcacccg gataatgcat
     8281 tcccatcttg atgcggctgt agccgacgac ccccgcccac gcatacgaag gaacaatcac
     8341 gtaccacttg ggataagcta aagaaagaga cgttgccata ttgaacgccg aactggtgtg
     8401 ccctgatgga aaagaagccg aacgttcggt gcttccgacc gtatgcacat tgttaagcgc
     8461 tttgtatgga cgttcacggt ttatcgccgc tttcatgctg taggttatag ccagattagc
     8521 cacggcggat gtcgtcatca gaatacccgc atcacgcatg tacgtgtctt ttcgcaaaag
     8581 cccggttgca acaaaactga gcggagtggc tatataaacg ggtttgacgg atcggctttg
     8641 aaattcataa aaacgatccc agccggggga atgatgttta ttgatggcct tgaataatcg
     8701 tgcgtcaata ttttgtgaat gcagcgaaac ctgtagcgat aaaacaaaac aaaaaaagcc
     8761 gagaatgatt ctcatatacc cttttttaag catgcgataa actcctttgg cggacgtaca
     8821 ggtttgtttt gacgatctgt aaatgcgtgg gtggtaaaac cgcgggcaac aggttcgctg
     8881 tcacgataac agatgtaagc tatggttgtg cgaatacctt cttgcgacgc aatccaggca
     8941 tcaatatgta ccgtatcgtc ataatgtacc gaacgcaaat aatccacttc gatacgaatc
     9001 acgggcataa aaataccttc ggattctaat cgtttgtaag agtagccaag ttcacggatg
     9061 agttcagtac gcccgacttc catccatggc aaataactgc catgataagc gacgcgcatg
     9121 ctatcggttt ccgcgtagcg aacgcggaca gtagttcgtg tgattttcaa atgatcaaca
     9181 taattttagg cactcatctc gccccagcga cccattttgg aatatttttc catacgctga
     9241 tcaagtaatt gctcaacaga aagtttttcc aattcggcaa aattatgcat tacagcttct
     9301 ttaagatatt gtgccgcaag atcatagttt cgattagcgc cgccaaccgg ttcttgtatt
     9361 ttatcgtcaa cgacatcaaa tcggatgaga tcatccgctg taagtttgag ttcggatgcg
     9421 gctctttctt tatattccca tgtgcgccat aaaatgctgg aacaggattc cggagcgatg
     9481 acagagtacc atgtgttttc catcatgaga attttgtcgc cgacaccgat acccaatgca
     9541 cctccggaag cgccttcacc gataactaca caaatgatcg gtacagggag gcgtgacatt
     9601 tccaaaagat tgcgtgcgat ggcttcagcc tgtccgcgtt cctcggcttc gatgccggga
     9661 tacgcaccgg gcgtatcgat cagggtaaga atcggtcgtt taaatttggc ggccatgtgc
     9721 atcaggcgca tggctttacg ataaccttcg ggattgggca taccaaaatt gcgataaaca
     9781 tggcttttgg tgtcttttcc tttttgctgt ccgatgatca taacttcttt tccgtcaatg
     9841 cgtcctattc cgctgacgat cgcatggtca tctccgaaaa ggcggtcccc gtgcaattcc
     9901 ataaacccgt cggatatacg ttcgatatag tcgagggatc gcgggcgctc gggatgacgc
     9961 gccagttgca ctttctgcca aggcgtgagg ttggcgtaaa tctctttacg cattgcttca
    10021 agtttatttt gcagcgcttc gatttccggt gttatgtcca cattggcatg ggccgacttc
    10081 atgtcggcga tctgcgcttc gagatcaaag atgggttttt caaaatccaa tattacaggc
    10141 acgttgattc tcctcctgat tcacaaaaaa aatttgctta tgactatacg caaaccgtca
    10201 aatgggttaa atcttaccaa aaaaatgttt tattcgcaat ttccaaacga gcaataaacc
    10261 ttcgcgaata atgttggtgg acatcttgga atatccgacg gtgcggtcgt aaaaaatgat
    10321 gggtgtttct atgattctaa atttttttaa ccatgttcga taattcattt cgatttgaaa
    10381 cgaataaccg ctggcactga ctttggtcaa atccatcgaa gatagcaccg aacgacggaa
    10441 gcatttataa cctgcagtag catccatgat cggcataccg gtaaccatac gcgcatacat
    10501 attaccaaaa tagctgatga gcaagcgctt gagcggccag ttgattacgt ttacgccgtt
    10561 tatatagcgt gaaccgatca ccaggtcatt atcgcgaata agacggaaca tatctttcag
    10621 ataacttggg tcatgcgaaa gatcagcatc catttcgagt atgtactcat acccgcgttc
    10681 cagagcccaa gagaatcctg ccacgtaagc tttaccgaga ccttgtttac ccggacgaga
    10741 taaaagatgt acccgtgtat cgtttttttg tatggtttga acgcgatctg ccgtgccatc
    10801 gggcgaacta tcgtccacga ctaatatctc cggtgcgacc tgatactctg aaaaatccaa
    10861 tgcaaaaatg cgttggatta aaacaggaat gttttctgct tcgttgtatg tgggaacaac
    10921 gatcagcgcg cgcgtcatag attgttcctt atacaaagcg gcacccgttt tgcttaaata
    10981 gtttaagacg atccatcagg tgattgtccg agaatgctaa aatctgtgcg gccaaaatag
    11041 cggcattttt gccgccggta ggatacgatg aaacgactgc aaccggcgct ccttccggca
    11101 tttcgacaga cgcgatggta gcatcccgat cggtaaaacc ttctttgccg atgggaatgg
    11161 caatcacagg aagatgtgtg taagccgcga tcatggccgc cagtttagat tgaaggctac
    11221 tggttgcaat aaaaattttt atgcctcgct ccggagcttt ctgcataaac tcctgcagct
    11281 ctggcggatc cagtaatggc gtgaaaactt tgacttcaca gtgcaatccg aaacgatcca
    11341 aagtttcttt acagcctttg atgtattccg attcctgtgt cgaatgaata ataatagcta
    11401 tttcggtcag attcataata tgacttttga ataaacttag ggcaaaaatt aacataccct
    11461 acgtaaacta caatacaaaa gttttaaggc atctttatac atgcttttgt ttgagacata
    11521 gatgagtttg taattttgtt aaaacaaatt tttctaaaat aaccggaact ttatgaatat
    11581 cttttcgtat gatcggtacc cgatatcatc ggaatctatt cttattttaa atatacctaa
    11641 atataccaaa ggagtgatca tgaagcgaaa tttgttaatg ttggcgtttg tcgtcgcgac
    11701 ggcatcgctt agtgcgggtg aacccaatgt caatccgctg atggccgggt ttaatcaaaa
    11761 catagaattc aatgttttga caccggagca catcaaaacc tcaaccgata tcatcatcga
    11821 aaaatccaaa acggcgttat cgggtatttt tgccattccc aaagaaaaac gctcgtttga
    11881 caacaccatg ctcgcgattg ataatatgta caacgactac ggcaaggttt catctaatat
    11941 ctatcttatg gccaatgccc acccggataa agcaacgcgc gaacaagcat tagccagcct
    12001 tagcgtattg gataaatttg ataatgaaat agcgctggat gaaaatttat accgcgcggt
    12061 gaaagaatat tccgaaagtc ctgaagctaa aaaactccaa ggattcaaaa agaaatatct
    12121 tgaagaaata cttcgcgatt tcgaacgaaa cggttttgct ttacccaaag aaaaaagaga
    12181 ccagttgaaa accatgcagg atcgcctttc cgatttgggt atcactttcg gtcgtaatat
    12241 cgcggaagcc gcggatacac tttttgtaac cgaagaagaa atgaaaggct taccggatga
    12301 ttataagagc gcacgtaaaa tgtccgacgg acgctat
//
LOCUS       DAOQGF010000101        12330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2386_length_12330_cov_6.618086, whole genome shotgun sequence.
ACCESSION   DAOQGF010000101 DAOQGF010000000
VERSION     DAOQGF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2386_length_12330_cov_6.618086"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7074)
                     /locus_tag="PK937_12330"
     CDS             complement(<1..7074)
                     /locus_tag="PK937_12330"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33405.1"
                     /translation="MKSILILTVLVILRGATLFSDVVAQEESSSAPKISTFATGKDIL
                     GVASNSVNLFSGDVHLPLALLSVPGRNGLDVGVNISYNSNIQNQNSTWNLEAPTGIMG
                     LGWSFGYEKIIVNHKSTGSIRDDDYYHISGSAGNQLIRTGTAPDGRLIYELKNYQFWK
                     IRRDTTYDWWEITKEDGTVFRFGGGVYSYNNRKYSEGNSVQWSVKWGNWVGSGTQDSL
                     QRQFATAWNLSKIKNISGDSVRFEYLNVDTTVKAGKPHTEASYLRRIIDVYGRRVEFI
                     LSDKLYNPSGQKEYQDPTTRFAEPDPYQDYYERKFLDSIEVFNDLNEKLMCIDLDYVI
                     LGSDSLAKRYLTSIIQRNAVYQSLPGIKFEYHTTGSLSGALKRITTPLGAKVEYTYTT
                     VNIKNSQRDLTVVRPQNCHDARVWTGDDYILVTWINNSDDSIKVAAYVWEGRWVYSGI
                     LATIGNVDYSLSQGYDFEAVIEKDFFGILVNYNRSSSTKYLHLFHKKPNAPDKWDYYT
                     QSLNVVNIQDNDVTFFSGERYVVVFGEESGKLFRFTWNGASWSSIEGPQLDSGSEDDF
                     FGTGAPDYFIFHDQNEGALDSIKFYYLDELGNWNIKLLNPTYSFNTDGGGDNPTYWYT
                     GPNFCVAMIKGQDDRIYWWSEEFDITQPHDTIVGLDYDSKVYINDAQISIHNMTSDHK
                     DKVRLYRFDGQKWVDKSIDNTTTHLYSATSSFGPDLAIRGSIQSEPAKIAEYNPSSGQ
                     WEERGLPNGGPRVGTRYYLFPNHDSIFYKMPNGQWRFAGKVSGDYTAGPRHGFNFTTC
                     THDSFPKKTGRAYILKNGQLKTTYISPYHTYNQYTSGILQNSDMTGAFACVFSTSSFD
                     TLVLHRVVGDVMTGRQQDFPVSSVTVYSGVDTTTVTYTYNDCTATYDPSGNFAEYHEV
                     KSTPSGGISGYTTTYFINSLPDTVAGFNVSYLDQEMKDNIGYLKGMQYYEQIFNASGV
                     ATGNTSQKYAITLKTLGAKAAGYYVRPKESISSRYGVKASTTYSYNVFNGLVAQTTTS
                     NRDASGNTDNIVKTFKYFYEAYDTTRTLNLLTPVIQTKDWVNNSVVATAATTWKDWGG
                     LRWAPYKSFVYQGTGSTDFNFSSYSGSSDPPVTEWIKINEIVSRIGGGPVKQSKNADG
                     IVSSFVYDYPYLNPIAAFVNARLSDTTLGSESSFLNFESNNETSGIKENDYWSFNASN
                     DFSTDAHSGVYSRKILPGSNVKGPSREFMPPDIKGQNRKYVFSCWVKTQSGFGSNNGR
                     LVIHSKRNADTAKTVYPNVPGAIATIYISDTQGQWKYIEAMIDLGKVRTDGGIGSGEL
                     IRLRDTISNNDATKYFLVDDIRFSPYDSPFSGIAYEKTYGLPVTSVGSNGEFSKTMYD
                     RFQRNIGTIVNDQFVPGTQMMYYSRKGNADTLNNNDLNAAVSVTASGAGKMDDFADGT
                     ADGWSLGTGIWTVTSGELTCTGSGANDYATLTGTGSAGVRAKVRSVNGGENYGIQIGN
                     VLFTRYSGAWKITIGSYSQTTSAMPSGTDWLAIVRDSVSLLYVDGKQIFNVRKWNTGG
                     NVSFYVGSFNTVAGFDDFLMFRDPGVALSYLDGSGKVVQSQFLDSVGTVVSATLYDPL
                     NRDAIVTKSAKYDSMFAFRKSFVSGFNWSSGIMTGYITTTGNAGSYPYSRLKFEDSPL
                     SGVIESSAPGDTFRIGRKTVKSAYGSNVTGDGFRDDLPASQYYMETSVDADGDSSWNL
                     TDQSGKVISRRSGSVFGRALLDSTAEVEFPEEIDTEPSLKQFTATVNQTCSYTCYLSG
                     GVGGVPSKSRTAYFKIGTTFGGSDIVSHSKTGNPGQNFSGTFSAQAGVTYYIEVSGYV
                     DVSIASGAVMYKSFGEGMYYPQTSYEYDDAGNLTRVYTPNYFTPPSGTALTDTNFIIR
                     FQYDYTGKLLSRKSPDGGTTRYMYDRLGRLRFSVNANGDAASPDIISYIKYDTYGRKT
                     EEGYYAYNWSGLTQTLADSASWPAPPTWRKKYSFDGDTSKPYMRGRLQKLQTNNDGDS
                     TLEVEESYTYDIFGRMTSKSLNVADFDAALYTSSYAYDFAGNITQMTYPSTGTQIRYS
                     YNASGALVAIGTPSDSDYFAGYRYYPDGALKEERLNNNTIIRSFTYNSAGWLTKIDDN
                     YFRENIDYIANGYGGAGYFDGNIAKTFYKYKWLPDSMTYQYKYDKFGRLLVADNSVSS
                     NWDMGVGQETVFDANGNIVKMKYGALAKTYAYYTGTNKIKNTDGSGNDYVYDNIGNVT
                     KSTPKTIDSLFYDPFINRTKQISKSTSKMTFQYDGTERRVYKKDSTSAISREFLYMYD
                     GLNSIAVKRKGGGTSEYVYGPTGLIATNINGTWYFVLKDHLGSVRTIVDQSG"
     gene            complement(7178..7435)
                     /locus_tag="PK937_12335"
     CDS             complement(7178..7435)
                     /locus_tag="PK937_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNH33406.1"
                     /translation="MKFSVYILYSKSCRRYYVGQTQDLERRLEEHNHGLSPSTRNGAP
                     WVIVYQFACDTRSDAMRIEKQIKKHSRIFEHGYPPMFKCTK"
     assembly_gap    7623..7722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7828..7902)
                     /locus_tag="PK937_12340"
     tRNA            complement(7828..7902)
                     /locus_tag="PK937_12340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7865..7867),aa:Pro,seq:tgg)
     gene            complement(7975..9327)
                     /gene="lat"
                     /locus_tag="PK937_12345"
     CDS             complement(7975..9327)
                     /gene="lat"
                     /locus_tag="PK937_12345"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HNH33407.1"
                     /translation="MEKIQIKPQEVHETLKKHILADGYDFVLDTKKSHGSWVVDSKTG
                     KEYLDFYSFFGSSPIGMNHPAMMTSEFREGLIDAATNNPANADVYTVAMAEFVATFEK
                     TGIPEYLPHMFMISGGALAIENALKTAFDWKVRKQHPNGFSSIEQEEDYVNSMKVIYF
                     KEAFHGRSGYTMTMTNTHYRYKVQYFPKFNWIKAPNPKMVFPLNDTNLADIKKREEES
                     LTFIREQVKRYKNQIAALILEPIQAEGGDNHFRVEFHKELRKICDENEIIMIYDEVQT
                     GVGLTGSFWAHEKIGVKPDILAFGKKMQICGILAGKRIDEVKDNVFQKSGRINSTWGG
                     NLADMYRATWYLRIIESEKLVENADKVGAHLLGKLQDLTKDFTQVTNVRGAGLMIAFD
                     LPTEQLRDAVHKKTPEFGLLVLKCGEKTIRLRPALNLTMAEADMGVERIAKALKEILK
                     "
     gene            complement(9505..10992)
                     /locus_tag="PK937_12350"
     CDS             complement(9505..10992)
                     /locus_tag="PK937_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNH33408.1"
                     /translation="MAQKFQNYINGKWQDAKSGATFENRNPASWDEVIGLFPKSGKED
                     VQEAVEAAAAAWDKWRLMPAPKRGDILRKVGDILVARKDEFAREMTREMGKVVAETKG
                     DVQEGIDTAYYAATEGRRLFGHTVPSELANKFAMSVRMPIGVAGLICPFNFPLAIPTW
                     KMFPALLCGNTVVFKPAEDTPKTGSMLLEVLIEAGIPAGVVNLVHGFGEDVGATIVSH
                     PKIDLISFTGSSQTGSMIASQCGKDLKHVSLEMGGKNAQVVMDDANLELALDGVLWGA
                     FGTTGQRCTATSRLILHEAIHDKFVDMLIKAAQKLVIGDGLKPGNQVGPLIHEESLKK
                     VQRYVEIGKSEGAKLALGGERHTDGDCAKGWFFKPTIFTDVKAGMRIEQEEIFGPVLS
                     VIKVKNIDEAITVLNGTKYGLSSSVYTKDVNNSFKAMRDIKTGITYINAPTIGAEAHL
                     PFGGVKATGNGHREGGWPVFDFYSETKTVYVDYSDKLQRAQIDNY"
     gene            complement(11017..11220)
                     /locus_tag="PK937_12355"
     CDS             complement(11017..11220)
                     /locus_tag="PK937_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33409.1"
                     /translation="MKMLWLVKLYWIWIVLGLIILIPAVIPPGLDMFNAFFGFAVVVV
                     GLVFRRAMQSDTLRQRIDRNLNS"
     gene            complement(11230..11904)
                     /locus_tag="PK937_12360"
     CDS             complement(11230..11904)
                     /locus_tag="PK937_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33410.1"
                     /translation="MQKRWFNIFSIISLVLFGAGDFILKASDKGEGQELHANRFLADA
                     LEDARHNYAGRTIPASAVVKFEVYRGFLADINKDGESEYIVAGTITGLDINYVMVYLW
                     KNEKWLCTVLNSGLGGGIADLQLLDVNQDGTEEIYSVIKDEKQKQHCRIDGFCKDLSS
                     ELSPMFCWNAEDGMSISQNILLIEKSGMRCVRVDEIRYPESEDGDIKQQTYYYVYENG
                     KFVRGQ"
     gene            complement(11914..>12330)
                     /locus_tag="PK937_12365"
     CDS             complement(11914..>12330)
                     /locus_tag="PK937_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNH33411.1"
                     /translation="YIVPIGKGDIKREGKDMTVVAYSSAVHWALEAAEIIEKEGVSVE
                     VLDLRSILPYDKELILQSVRKTNRVIVAHEATLTGGVGGDIAAFITEHAFESLDAPVK
                     RLAAIDTPVPYSPPLEAYFLPNKDKMLAMMRELAAY"
BASE COUNT         3195 a         2992 c         2534 g         3509 t
ORIGIN      
        1 gcccgattga tccacgatcg tacgcaccga acccaaatga tctttgagta caaaatacca
       61 cgtcccgttg atattcgtcg caatcagtcc cgtcggacca tagacatatt cgctcgtacc
      121 cccgccctta cgctttacgg cgatgctatt aagtccgtca tacatataca aaaactcacg
      181 gctgatcgcc gaggtactgt cttttttata tacccggcgc tccgtaccat cgtactggaa
      241 tgtcatctta ctcgtgctct tggatatctg tttggttcga ttgatgaagg gatcgtagaa
      301 aaggctgtcg atcgtttttg gggtagattt cgtcacattc ccgatattat catacacgta
      361 gtcattccct gaaccgtcgg tattcttgat tttattggtt ccggtgtagt atgcgtaggt
      421 cttagccaaa gccccatact tcatcttcac gatgttacca ttggcatcaa agactgtttc
      481 ctgaccaaca cccatatccc aattggaact gactgaatta tcggctacaa gtaatcttcc
      541 aaatttatcg tatttgtact gatacgtcat gctatccgga agccacttat atttgtaaaa
      601 cgtcttcgca atgttaccgt caaaatatcc cgcaccaccg tacccattgg caatatagtc
      661 gatattttcc ctgaagtagt tatcatcgat cttcgtcagc cagccggccg agttatatgt
      721 aaagctgcgg atgatcgtat tgttattgag tcgttcctct ttcaatgcgc catcgggata
      781 ataacggtaa cccgcaaaat aatccgaatc cgaaggtgtg ccgatggcta ccaaagcgcc
      841 ggaagcgtta taggaatagc gtatctgcgt ccctgtggaa ggatacgtca tctgagttat
      901 atttccggca aaatcatagg cgtatgaaga agtatagagt gcggcatcaa agtcggctac
      961 gttgagggac ttggaagtca ttcgaccgaa tatatcatag gtataagatt cctccacctc
     1021 cagcgtactg tccccatcgt tattagtttg aagtttctgt agtcgtcctc tcatatacgg
     1081 tttggacgta tcgccgtcaa aggaatattt ttttcgccac gtcggcggag caggccatga
     1141 cgcgctgtcg gccagagttt gcgtcagacc ggaccagtta taagcatagt acccctcttc
     1201 tgttttgcgc ccataggtat cgtatttgat gtatgatatg atatccggcg atgccgcatc
     1261 gccattggca ttcactgaaa aacgcaatcg ccctaagcgg tcgtacatat aacgcgtcgt
     1321 acctccatcg ggtgatttgc gggacagcag cttacccgta tagtcgtatt ggaaccgtat
     1381 gatgaaatta gtatccgtca aagcggtacc cgacggcgga gtaaaataat taggcgtata
     1441 gacgcgcgtc aagtttcccg cgtcatcata ctcatacgat gtctgaggat aatacatacc
     1501 ctcgccgaag gatttgtaca taaccgctcc cgacgcgatg gaaacatcta cgtatccgga
     1561 cacttcgata taataggtaa cgcccgcctg cgccgaaaat gtccctgaaa aattctgacc
     1621 cggattaccc gtcttgctgt gcgacacaat atcggagcct ccgaatgtag taccgatctt
     1681 gaagtaagcc gttcggcttt tcgatggaac ccctccgacc ccgcctgaca gatagcatgt
     1741 gtatgagcat gtttgattga ctgtagcagt aaactgtttt aaagatggtt ctgtgtcaat
     1801 ttcttcagga aactcaactt ccgctgtgga atccagtaat gctctgccga atacagaacc
     1861 cgatcttctg gaaatcacct tgcctgattg atcggtaagg ttccagcttg agtcaccgtc
     1921 cgcatcgacg gatgtctcca tataatattg actggcagga aggtcatcac gaaatccgtc
     1981 gccggttaca ttagagccat atgccgattt tacagtttta cgtccgatcc tgaacgtatc
     2041 tccgggggcc gaagattcga taactcccga cagcggtgag tcttcaaact ttagtcggct
     2101 gtaagggtaa ctgcccgcat tgcctgtagt cgttatatat cccgtcataa tgccgctcga
     2161 ccaattaaat ccggacacaa atgatttacg aaatgcaaac atggagtcgt acttagccga
     2221 tttggtgacg atggcatcac gattgagtgg atcatataaa gtggccgaaa cgacagtacc
     2281 tacgctgtct aaaaactgag attggacaac cttgccgcta ccatctaggt aactcaaggc
     2341 gacaccggga tctctgaaca ttaaaaaatc atcaaaccct gcgacggtat taaaagagcc
     2401 aacataaaaa ctgacattac ctccggtatt ccacttccgg acattaaaaa tctgtttacc
     2461 gtccacgtat aacagcgaca cggaatctct cacgatggca agccagtctg tcccggaagg
     2521 catcgccgat gtcgtctggg aataactccc gatagtgatt ttccatgcgc cggaataccg
     2581 tgtaaaaagt acattaccga tttgaatgcc ataattttca ccgccattga cagatcgcac
     2641 ttttgcgcgt acaccggcgc tacccgtacc cgtgagtgtc gcgtagtcat ttgcaccaga
     2701 accggtacaa gtgagttccc cgctggtcac cgtccatata cccgttccca gagaccaccc
     2761 gtccgccgta ccgtcagcga agtcatccat ctttcctgcc ccgctcgccg tcacggatac
     2821 ggcggcattg aggtcattgt tattgagcgt atcggcgttc ccttttcggg agtaatacat
     2881 catctgagta cccggcacaa actggtcatt gactatcgta ccgatattcc tttgaaaccg
     2941 atcatacatc gttttcgaga attcgccatt ggatccgaca gacgtcacag gaagtccgta
     3001 ggttttttcg taagcaatgc ctgaaaacgg agagtcataa ggagaaaaac ggatgtcatc
     3061 caccagaaaa tatttagtag catcgttatt agaaatcgta tcccgtaacc tgatcaattc
     3121 gcccgatccg ataccgccgt ccgtacgcac ttttccgaga tcgatcatgg cctcgatgta
     3181 tttccattgg ccttgggtat cgctgatata tatcgtggct atcgctccgg gcacgttggg
     3241 ataaacggtt ttagccgtgt cggcattacg tttggagtga atgactaatc ttccgttatt
     3301 tgatccgaat ccggactgtg tctttaccca acatgaaaaa acatatttgc gattttggcc
     3361 tttgatatcc ggcggcataa attcgcgcga cggtcctttg acattactac ccggtaaaat
     3421 cttacggcta tacacgccgg aatgcgcgtc ggtcgagaag tcattagacg cattgaatga
     3481 ccagtagtca ttttctttga taccggaagt ttcgttattg ctttcaaaat tcagaaaact
     3541 cgactcgctt ccaagcgtcg tatcggataa tctcgcattg acgaacgccg caatgggatt
     3601 cagatacgga taatcataca cgaaagatga aacgatacca tccgcatttt tgctttgttt
     3661 gaccgggccg ccaccaatgc gtgatacgat ttcgttgatt ttaatccatt ccgtgacggg
     3721 agggtctgat gatcccgagt aggaagaaaa attgaaatcg gtcgaaccgg ttccttgata
     3781 tacaaaactt ttatacggcg cccaccgaag tcctccccaa tctttccaag tcgtggctgc
     3841 cgtcgcgact accgaattat taacccaatc tttggtttgg ataaccggcg tcaaaagatt
     3901 aagtgtacgg gttgtatcgt aagcttcata aaaatattta aacgttttga cgatgttatc
     3961 ggtattgccg gatgcatcgc ggttacttgt cgttgtttga gccacaagcc cgttaaaaac
     4021 attataactg tatgtcgtgc tggccttcac gccatagcgg gagcttatgg attcttttgg
     4081 gcgaacataa tacccagccg ccttagcgcc caaggttttt aaagtaatcg catatttttg
     4141 tgatgtattc ccggtcgcta cgcccgatgc attaaaaatt tgctcgtaat attgcatgcc
     4201 tttcagataa ccgatattgt ctttcatctc ttgatctaaa taggatacat taaaaccggc
     4261 tactgtatcc ggaaggctat tgatgaaata cgtcgtcgtg tatccggaga taccgccgga
     4321 aggtgtggat ttgacttcat gatactcggc aaagttacct gaaggatcat acgtcgccgt
     4381 acaatcattg taagtataag tcaccgtagt cgtatccaca ccgctataga ccgttactga
     4441 tgacacagga aaatcttgct gacgccctgt cataacatca cccaccacgc gatgcaatac
     4501 caatgtatcg aaagacgacg tgctaaagac gcacgcaaaa gctccggtca tgtcactgtt
     4561 ttgcaggatt ccgcttgtat attgattata ggtatggtaa ggcgatatgt aagtggtttt
     4621 taattgaccg tttttaagga tatacgctcg gccagttttt tttggaaaag agtcgtgtgt
     4681 gcacgttgta aaattaaagc catgcctcgg tcccgctgta taatcacccg acaccttacc
     4741 tgcaaaacgc cattgcccat taggcatttt gtaaaaaata ctatcatgat tgggaaataa
     4801 ataatatcgt gtacccactc gaggtcctcc attgggtaac cctcgctcct cccattggcc
     4861 cgaggacgga ttgtattccg caatttttgc cggttctgat tgtatggagc cgcgtatggc
     4921 aagatccggt ccaaatgaag atgtcgcgct atagagatgg gtggtcgtat tgtctattga
     4981 tttatccacc catttttgac cgtcaaaccg ataaagtctt actttatctt tatgatcgga
     5041 cgtcatattg tgaatgctta tttgcgcatc attgatatac actttagaat catagtcaag
     5101 acccactatg gtatcatgag gttgagtgat atcaaactct tccgaccacc agtagattct
     5161 gtcatcttga cctttgatca ttgctacgca aaaattgggt ccggtatacc aatacgttgg
     5221 attatcccct ccgccatccg tattgaaact ataggttggg tttagtagtt taatgttcca
     5281 gtttcccaac tcatccaggt agtagaactt gatgctatcc agtgcgcctt cattctgatc
     5341 atgaaaaatg aagtaatccg gcgctcctgt tccaaaaaaa tcgtcctcgc taccggaatc
     5401 caattgcggg ccttcgatgg aagaccatga agcgccattc caagtaaatc tgaacaattt
     5461 gcctgattct tctccgaaaa caaccacata ccgctctccg gaaaagaagg taacatcatt
     5521 atcctgaata tttactacgt tgagcgattg ggtataataa tcccatttgt ccggcgcatt
     5581 tggtttcttg tgaaataaat ggagatattt tgtagaactt gagcgattgt agttaaccaa
     5641 aataccaaaa aaatcctttt caatcaccgc ttcaaaatca tatccttggg ataaactata
     5701 atcaacgttg ccgatcgttg ccaaaattcc gctataaacc caccgccctt cccacacata
     5761 ggccgcaacc tttatcgaat catcgctatt attgatccat gttacgagta tgtagtcatc
     5821 tccggtccat accctcgcat catggcagtt ttgaggacgt actaccgtca ggtccctttg
     5881 cgagtttttt atattaacgg tagtataggt gtactcaacc ttagctccta acggcgtggt
     5941 tattcgtttt aatgcgccgg ataaactacc ggtggtatga tactcaaatt taattcccgg
     6001 taatgattga taaactgcat ttctctgaat tatagaagtc agataccgtt tggcaagact
     6061 gtcgcttccc agaataacat aatccaaatc aatacacatc agtttttcgt ttaggtcatt
     6121 aaatacctct atggagtcta agaattttct ttcataataa tcctgataag gatcgggttc
     6181 ggcaaaacgg gttgtcggat cttgatactc cttctgaccg gatgggttgt ataatttgtc
     6241 agaaagaata aattcaacac gacgtccgta gacatcaatg atgcgtctta gataagatgc
     6301 ttctgtatgc ggcttacccg cttttacggt tgtgtctaca ttaagatatt cgaatcgaac
     6361 cgaatctccg gagatgtttt taattttaga tagattccat gccgtggcaa attgtcgttg
     6421 cagggagtcc tgagtacctg atccgaccca attaccccat ttgacactcc actgaacaga
     6481 attgccttca gaatatttac gattattata agaatatacg ccccctccga atctgaaaac
     6541 ggttccgtct tcctttgtta tctcccacca gtcataggtc gtatctcgac gaatcttcca
     6601 aaattgataa tttttcaatt catatataag ccgcccgtct ggggccgtac ccgttcttat
     6661 gagttgattg cctgctgaac cactgatatg gtagtaatca tcgtcgcgga tggagccggt
     6721 cgatttatga tttactatta ttttttcgta tccgaaactc cagccaaggc ccataatgcc
     6781 ggttggcgct tccaaattcc atgtggaatt ttgattttgg atgttactgt tgtacgaaat
     6841 attgacaccg acatccaagc catttctgcc tggaaccgat aaaagtgcaa gaggtaaatg
     6901 cacatccccg gaaaacagat tgacgctatt tgaagctacc ccaagaatat cctttccggt
     6961 agcaaacgta gaaatttttg gtgcagacga gctttcctct tgagcgacga catcagaaaa
     7021 taacgtagca cctctcaaaa tcactaatac tgttaggatt aaaatggatt tcatactccc
     7081 cctatttttt tatcgtagtt atgacgcgtc gagatatatg gtaaggctac cgtactacat
     7141 aagaaaaact aaaataaacc ccatgtcaag ctttttttta cttcgtacat ttgaacatcg
     7201 gcgggtaacc gtgttcgaaa atacggctat gttttttaat ctgtttctcg attcgcatcg
     7261 catctgatct tgtatcgcat gcaaactgat aaacgattac ccacggcgcg ccatttcttg
     7321 tggaaggaga taatccatga ttatgctcct caagcctacg ctctaaatct tgagtttgcc
     7381 caacataata tcgccggcaa cttttactgt acaatatata tactgaaaat ttcattctaa
     7441 cgaaaacagc cacgttttaa cgtggctgtt actcatttct ctctgtcggg atgaggggat
     7501 tcgaactcct gatcacgcct ttggcgtgac tcccaaagct tgccgttcaa tatatcgcgc
     7561 tgcgccttgt tttttaatct gtttctcgat tcgcatcgca tctgatcttg tatcgcatgc
     7621 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaacataa tatcgccggc
     7741 aacttttact gtacaatata tatactgaaa atttcatcct aacgacaaca gccacgtttt
     7801 aacgtggctg ttactcattt ctctctgtcg gggtgagggg attcgaactc ctgacatcct
     7861 gctcccaaag caggcgcgct aaccgggctg cgctacaccc cgaacctatc tatcaaacaa
     7921 cccgtgttta agatcacttt tagcacgggc gtctgatata cggtaaatta tttattattt
     7981 caagatttct tttaatgctt tcgcaattcg ctcaacgccc atgtcggctt ccgccattgt
     8041 aaggtttagt gccggtcgaa ggcggattgt tttttcaccg catttcaaaa ccaacaaacc
     8101 gaattccggc gtttttttat ggacggcatc acggagttgt tcggtcggta aatcaaatgc
     8161 gatcatcaga cccgcgccgc gtacattggt aacttgtgta aaatctttgg tcagatcttg
     8221 cagtttgcca agcaaatgag ctccgacttt atccgcattt tctaccaatt tttctgattc
     8281 tataatacga agataccacg tcgcacggta catatccgcc aaatttccgc cccaggtcga
     8341 attgattcgt cccgattttt gaaatacgtt atcctttacc tcatcaatac gtttgccggc
     8401 aagaataccg catatctgca tttttttacc aaacgcaaga atatcgggtt taacgccgat
     8461 tttctcatgc gcccaaaagc taccggttag accgacgccg gtctgcactt catcgtagat
     8521 catgatgatt tcgttttcat cacagatttt tcgcagctct ttgtgaaatt caacacgaaa
     8581 atgattatcc ccgccttcgg cctgaatcgg ttctaaaatc aacgcagcga tttgattttt
     8641 ataacgcttc acttgctcgc gaataaatgt tagcgattct tcttcccgtt ttttgatgtc
     8701 ggcaagattg gtatcattta aaggaaatac catcttgggg ttcggcgctt tgatccagtt
     8761 gaatttcggg aaatactgca ctttataccg ataatgcgta ttggtcatcg tcatcgtata
     8821 accactacga ccgtgaaatg cttccttgaa ataaatcact ttcatggaat tgacgtagtc
     8881 ttcttcttgc tcgatcgaac tgaacccgtt gggatgttgc ttacggactt tccagtcaaa
     8941 cgccgtttta agcgcatttt cgatggccag agcgccgccg gaaatcataa acatgtgagg
     9001 aagatattcg ggaataccgg ttttttcgaa tgtcgcaacg aattcagcca tcgctaccgt
     9061 atatacatcg gcattcgcag gattattagt cgcggcatca atgagacctt cacgaaattc
     9121 agatgtcatc atggccgggt gattcatacc gatcggtgac gatccaaaaa acgaataaaa
     9181 atccaaatat tctttaccgg ttttagaatc tactacccat gaaccgtggc tcttttttgt
     9241 atcgagtaca aaatcgtaac catcggcgag aatgtgtttt ttgagtgttt cgtgaacttc
     9301 ttgcggtttg atttgaattt tttccatgtc ggtatccttt tttaataatt aaaaattatc
     9361 tacaaaaaag aaccgatctc acacgcgcca ttccggtttg agcaagcaat gacgcacttc
     9421 gccgacagct tcttgttgca gaagccatcc ccgacgttgc gctgtgccgt tggatgtatt
     9481 ttggaaaaat tcactcatgg ttttttagta attatcaatc tgtgcacgtt gcagtttatc
     9541 gctatagtcc acgtatactg ttttggtttc actgtaaaaa tcaaataccg gccaaccgcc
     9601 ttcacggtgt ccgttacccg tagctttgac accgccaaaa ggcaaatgtg cttcggcacc
     9661 gatcgtcggt gcattgatat acgtgatacc ggttttgatg tcgcgcatgg ctttgaagct
     9721 gttattgaca tctttggtat agaccgatga agataagccg tatttggtac cgttaagcac
     9781 cgtgatcgct tcatcaatat tttttacttt gatcactgag agcacgggac cgaaaatttc
     9841 ttcttgttcg atacgcatgc ctgctttgac gtcggtaaaa atcgtgggtt taaagaacca
     9901 tcctttggca caatcgccgt cggtatgacg ttcaccacca agcgccagtt ttgcaccctc
     9961 gctcttaccg atctcgacat aacgctgaac ttttttgagt gactcttcgt gaatcaacgg
    10021 accaacttga tttccgggtt taagaccgtc accgatgaca agtttctgag ccgccttgat
    10081 cagcatatct acaaatttat catgaatcgc ttcatgcaaa atcaatcggc tcgttgcggt
    10141 acagcgctgt cctgtcgtac cgaatgcgcc ccataaaacg ccgtcaagag ccaattcgag
    10201 attggcatcg tccataacga cttgtgcatt ttttccgccc atctcaaggg aaacatgctt
    10261 aaggtcttta ccgcattgtg atgcgatcat actgccggtt tgggacgaac cggtaaagct
    10321 gatcagatca attttcggat gacttacgat cgttgcaccg acatcttcac cgaaaccgtg
    10381 cacaaggtta actacgcctg ccggaattcc cgcttcgatc aaaacttcga gcaacatact
    10441 tccggttttc ggcgtgtctt cagccggttt gaaaacgaca gtattcccgc aaagcagcgc
    10501 cgggaacatc ttccacgtcg gtatggctaa cggaaaatta aacggacaaa tcaaccccgc
    10561 aacaccgatc ggcatacgaa ccgacatggc aaatttattg gccaattccg aaggaacggt
    10621 atgaccgaaa aggcggcgcc cttcagtcgc tgcgtaatag gctgtatcaa taccttcttg
    10681 tacatcgcct ttcgtttcgg ctacgacttt gcccatttcc cgtgtcattt cacgggcaaa
    10741 ctcatcttta cgcgcaacaa gaatatcgcc gactttgcgc aatatgtcgc cgcgtttggg
    10801 agccggcatc aaacgccatt tatcccatgc ggccgcggcc gcttccacgg cttcctgcac
    10861 atcttctttt cctgatttgg gaaataaacc gatcacttca tcccaactag cgggattacg
    10921 attttcaaag gttgcaccgg acttggcatc ttgccatttt ccgttgatat aattctgaaa
    10981 tttctgagcc atgaataaat tcctataaat aaaatgttac gaattcaaat tgcgatcaat
    11041 gcgctggcga agcgtatcgc tttgcatcgc acgtcgaaaa accaatccga caactactac
    11101 cgcaaaacca aagaatgcat tgaacatgtc cagccccgga ggaataacag ccggaattaa
    11161 aataatcagg ccgagaacta tccatatcca atataacttt acgagccaca acattttcat
    11221 attacaccac tattgtcccc gtacaaattt tccgttttca tatacataat aatacgtttg
    11281 ttgcttgata tctccgtctt ccgattccgg ataccggatt tcgtccaccc gcacacagcg
    11341 catcccgctt ttttcgatga gcagaatatt ttgtgaaatg ctcattccat cttctgcatt
    11401 ccaacaaaac atcggtgaca attcgctgct caggtcttta caaaatccgt cgatacggca
    11461 gtgctgtttt tgtttttcat ctttgatgac cgagtatatt tcctctgtgc cgtcctgatt
    11521 cacatccaac aattgtaagt cggcaatgcc tccaccgaga ccgctattga gaactgtaca
    11581 gagccatttt tcgttcttcc acagatacac catgacgtaa ttgatatcca ggcccgtaat
    11641 cgtgccggcc acgatatatt cggattcgcc gtctttattg atatccgcaa gaaaaccgcg
    11701 atacacttca aacttcacga cagccgatgc gggaatagtg cgacctgcat aattatgacg
    11761 ggcgtcttcc aaagcatccg caagaaaacg gtttgcatga agttcctgtc cctcgccttt
    11821 atccgaggcc ttcaaaataa aatcaccggc accaaagagt accagtgaaa ttattgaaaa
    11881 tatattaaac catcgtttct gcatgatgtt cgatcagtaa gccgcgagct cgcgcatcat
    11941 cgccagcatt ttgtctttat tgggaaggaa atacgcttcg agcggcgggc tgtacggcac
    12001 gggtgtatca atcgccgcaa gtcgtttcac cggcgcatcg agcgattcga aagcgtgttc
    12061 ggtgatgaac gctgcaatat cgccaccgac cccgccggtt agcgtagctt cgtgagcgac
    12121 gatgactcga ttggttttgc gaaccgattg gagaatcagt tccttatcgt acggtaaaat
    12181 gctgcgcaga tcgagcactt cgacactgac gccttctttt tcgatgatct ctgccgcttc
    12241 aagcgcccaa tgtacggccg aactgtacgc cacgaccgtc atgtctttac cttcgcgctt
    12301 gatgtcccct ttgccgatcg gcacgatgta
//
LOCUS       DAOQGF010000102        11349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2640_length_11349_cov_6.228706, whole genome shotgun sequence.
ACCESSION   DAOQGF010000102 DAOQGF010000000
VERSION     DAOQGF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_11349_cov_6.228706"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PK937_12370"
     CDS             complement(<1..678)
                     /locus_tag="PK937_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33412.1"
                     /translation="MQNQLPIPHPDPLGVPGDIGLILFLLYLTFFLHVILMNALLGGA
                     FMAVISEWISRKTNNQQTKDHHIHLAEKLGHILPFSVSLTVSMGIAPLLFVQVIYGQF
                     FYTSSVLMAWIWLSVVFLIVIGYYSLYAYTRGFERWANRRYIFMIISCVMFFLIAFIY
                     VNNLTLMQTPQKWLDIYTQTNGTGYHWNMDEATLMPRYLHFVFSAIAVAGMLVLLLGM
                     REKKDEAL"
     gene            complement(695..2071)
                     /locus_tag="PK937_12375"
     CDS             complement(695..2071)
                     /locus_tag="PK937_12375"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNH33413.1"
                     /translation="MPEYPVWNVGGVGHGWIIGLIAMTHVYISHFAIGGGLYMAVTEA
                     LAVKRNDQAMIGYLRSHSNFLLLLTTVLGAMGGIGIWFAVGLVNPASIQALIHNFMFF
                     WAIEWVVFVVEIAIAFFYYYSWDKVSPQFHVKLAWLYFIISAFSLLVINGILTFMLTP
                     GKWLETGNVWDGFFNPSYFPSVIVRFLIMCTIAGVFSIFTSSRVKDENLRTRLLHYSA
                     KWLMPSFFLLPFFLYWYFTSLPADVAKLLETGIANIGAGNLSVLTRVLLLTMLLSVTI
                     YVIALVGPFFNPKIFTFGFSVAILILGLIVTGATESIREMIRKPYTVYGYLYSNGTLK
                     KASADSSNYGILKHYPWIAEKEITPANMKTVGQKIFLAQCQSCHTPTGYRSMKDLAKG
                     WDREFIFRRLTTLPATGIMPPFMGDEQERRALADYIGDIVGAGPWPPPDSTPATTPAP
                     APTASIKN"
     gene            complement(2101..3207)
                     /locus_tag="PK937_12380"
     CDS             complement(2101..3207)
                     /locus_tag="PK937_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNH33414.1"
                     /translation="MSNEMANKNHDDPNIAFLPKFIRERYDVGWIVAFFKHKEVPVHK
                     HSLWYYMGGISLFLFLVQIVTGVLLMVHYNAGEAHASVQAIMSQVDFGWLIRSTHSWS
                     ANLMVFFVFLHMFSVFFMKSYKYPREMTWMTGMILLAMCLGFGFSGYLLPWDQLAYFA
                     TQIGVKTTEAVPVMGGFIADMLRGGKELGTQTIERFYALHVWVLPALFMMTLGLHLLF
                     VQLQGIHAPEFFENQPADKKKYMKFFPDFAIKDVYVWLIVMNFLGLLAVMFPWELGAE
                     ADPLAPAPAGIKPEWYFLAMFQLLKLFPPEIGPIEGELFGIMLFSLVGVLWAAIPFID
                     SRTAPAWRSRATFYFGIAFLTGFIIFTMWGHYSK"
     gene            complement(3232..3699)
                     /locus_tag="PK937_12385"
     CDS             complement(3232..3699)
                     /locus_tag="PK937_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNH33415.1"
                     /translation="MKDTKDPSEHIHMPDKRDFMRTVFTGGFMLWIGAFLYPVYRYLK
                     PRVEEDTANAVQSVVAGKVADFDKVRAKLIKFGSKPVLLLKNDAGELVALGATCKHLG
                     CTVQYASDKNHIFCGCHGGIYDLTGKNIAGPPPAPLDKFKVVTNGDEIVIHRV"
     gene            3960..5048
                     /locus_tag="PK937_12390"
     CDS             3960..5048
                     /locus_tag="PK937_12390"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNH33416.1"
                     /translation="MDRKISFAPVQGAFSQVLKERVQQYFAEKGISMRGGLHMAIKSG
                     FYLSLAVMTYILIISGILSVWGLLLAAMLMGFLVACIGFNVGHDAIHGAMSHRKWVNT
                     LMGLTFEIFGASVYLWRMYHNVIHHTYTNIPGADRDIDGRPLIKLAPSQPTYKIHRFQ
                     YIYAFAVYTLTSLNWVLRKDYQRMFMKHNGVYTTKMPSVPLIASMFMFKLVHFTLFLI
                     LPIIVLPVPFWMVIIGFFVMHMTTGITLALVFQMAHVVEGMEFPEPHEDGMLENEFFV
                     HQLKTTANFSRKSKIATWICGGLNLQVEHHLFPHICHTHYPAISDIVKRTAQEFGIPY
                     HENTSFWSALASHVRHLRKVGYIPVQLG"
     gene            5209..6054
                     /locus_tag="PK937_12395"
     CDS             5209..6054
                     /locus_tag="PK937_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH33417.1"
                     /translation="MNTYLKNIVEYYHATEKAYHLHWDVKNSLSVHYGYWDDKVSSFS
                     ESLLRMNEVMAEAVGITSSDRVLDAGCGVGGSSIYLAKTRGCRAVGITLSDRQVAQAE
                     TFAKDRGVSEQTEFKIMDYCHTDFPDASFDVVWGCESICYAESKEQFIREAYRLLKPG
                     GRLVIADGMTAKFENNDHPINRRWLEGWAANFIETPERMQGYMQHMGFTDIRFRDITP
                     HVMHSSRRMYMLYFPAKVYHAVVRMLGRPSPLLFRKNVDACRFQYIAFKRKLWMYGLL
                     CGVKK"
     gene            6067..6915
                     /locus_tag="PK937_12400"
     CDS             6067..6915
                     /locus_tag="PK937_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH33418.1"
                     /translation="MSNYHDQIVDYYRETENHYRVYWALDKTLAIHYGYWDEKVKSFP
                     QSLLRMNEVLAETAKIQKTDRVLDAGCGVGGSSIFLAQHIGCHATGITLSAKQVARAF
                     ENAKNKNVSDRTDFKVMNYCKTDFPDASFDVVWGCESICYADSKEQFVHEAFRLLKPG
                     GRLILADFMVPAFENNQDPSVRRWLDGWVVNFLETPDNFKKYMLEAGFEDVRFRDIST
                     HTLHSTKRLGRLYYWGKVYLAYCKIIGKTMDPIRLRNIKSAYDQYQSHRRALWIYGMF
                     CTTKPS"
     gene            6981..7826
                     /locus_tag="PK937_12405"
     CDS             6981..7826
                     /locus_tag="PK937_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH33419.1"
                     /translation="MSAYHQNIIAFYDVLENPLRMIWDLDKSYAIHYGYWDEKVKSFP
                     ESLLRMNEVMAETAQIKSHERVLDAGCGVGGSSIFLARTIGCKTVGITLSANQVQRAK
                     KNAQNSGTAHLNEFKVMDYSHTDFEDASFDVVWGCESICYADNKEEFVKEVYRILKPG
                     GRFIMADGMVQKFEDNDNPIVAKGRDGWAANYVETPERFKQFLENTGFTNITYRDITD
                     HIMHSAKRLYWVSFPAKLNQWIRPLFGKTVAEHNKGNVNACKYQYLAFKKRLLVYGIL
                     CGVKR"
     gene            7860..8708
                     /locus_tag="PK937_12410"
     CDS             7860..8708
                     /locus_tag="PK937_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH33420.1"
                     /translation="MNSYQQKIVQYYHATEKSYKDGWGLKKNLMIHYGYWDDKVSSFS
                     ESLLRMNEVMAEAVGITSSDRVLDAGCGVGGSSIYLAKTRGCRAVGITLSDRQVAQAE
                     TFAKDRGVSEQTEFKIMDYCHTDFPDASFDVVWGCESICYAESKEQFIREAYRLLKPG
                     GRLVIADGMTAKFENNDHPINRRWLEGWAANFIETPERMQGYMQHMGFTDIRFRDITP
                     HVMHSSRRMLYFYFPTRCYHLYQYLIRKSVPEVNRKNIDALLYQYIAFKKKLWLYGML
                     SGVKHQ"
     gene            complement(8787..9422)
                     /locus_tag="PK937_12415"
     CDS             complement(8787..9422)
                     /locus_tag="PK937_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33421.1"
                     /translation="MNRRGFFNNIFKSDEPSGPIHFYGIQIVINTGINDETRAKLHAA
                     INVPVKEGETPEEKKAFFKNVAALLLENEPFFEYGYWDYLTDAAEAQAEFDSWVNEIE
                     SSMATESGEVGETMDEQYRMSAEKQYVVISLAFLIENVPTQESFQGMIDIPEESYFLR
                     ETWPKLIEAVPYLDFNYVMADAVFIMPGNEKDGFSFEDLHSEGWEYLKPIS"
     gene            complement(9440..10972)
                     /locus_tag="PK937_12420"
     CDS             complement(9440..10972)
                     /locus_tag="PK937_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33422.1"
                     /translation="MAKRKTKRNQKAQTPAWWNAMISRREAGQRLATLATGAAMLPFA
                     SILTGCGEEVELDALELQQKQGWQVGADGENLDFKDEQSRDSLGQAWENYADPAALMV
                     AYEPKNYRWKPFKVTTLVQSLSQETLRKQVKPILSDAMKTAYARGLGMREIIQKMDNP
                     SSTMIIADLHGEESVAFGAALADVANVVIGFDNWPHPQGVVPAHRTLGAMMYYAREVS
                     VKTEEKLSWEAPAVILLDNHRLGEYTDEWDVFDNRYVAKVPSAPSLASYKVNRVLYVI
                     PDRDTKNELDDLNDDFVYYKEKNISVDLLPMSDFKPAVASAMATSASAKTSADSMIAA
                     FTGSDSAIAKAMTAPAQKDTVVATPASTRTVYHYGGGPSYWPIFFGNYNTPSTISRPG
                     GYRAPSQGPSKPEYKPERRPTLFSSRTVGSQTSGVGKQKPTGFGRVSANVNSDGNVTR
                     VRQSRNYSSTGTSRASRSTSTSSSSRSSRSYRSSSSKSSSSRSSSGRSGSFGRSRSSS
                     SS"
     gene            complement(10983..>11349)
                     /locus_tag="PK937_12425"
     CDS             complement(10983..>11349)
                     /locus_tag="PK937_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33423.1"
                     /translation="ARNTIRTWIPETRRLITKESKKLNKNEWVLKSDYGCEGDEVIIG
                     NDVSETLWKESLSKAIPKHWILQRYFSSIRIGRENLIPNFGLYLVGGRAAGIYTRLSN
                     TATDGTALSAATLIDQHSH"
BASE COUNT         3052 a         2512 c         2810 g         2975 t
ORIGIN      
        1 aagcgcttca tcttttttct cccgcatacc cagtaacaaa actaacatac ctgctacggc
       61 gattgccgaa aatacaaagt gcagataacg cggcatcagg gtggcttcat ccatattcca
      121 gtgatacccc gtgccgttgg tttgcgtata aatgtctaac catttttggg gcgtctgcat
      181 gagcgtgaga ttattgacat agataaaagc gatcagaaaa aacatcacgc aacttatgat
      241 cataaagata taacgccgat tggcccagcg ttcaaatccg cgcgtgtagg catacaaact
      301 gtagtaaccg atgacgatca gaaacacgac ggacagccat atccacgcca tcagtacgga
      361 cgaggtataa aaaaattgcc cgtatatcac ttgcacgaaa agcaaggggg cgatgcccat
      421 cgataccgtg agtgaaacgc tgaaaggaag tatatggcct aatttttctg cgagatggat
      481 atggtgatct ttggtttgct gattatttgt ttttcgggaa atccattcgg atatcaccgc
      541 catgaatgcg ccaccaagga gcgcattcat caggatgacg tgtaaaaaaa acgtgagata
      601 cagtaaaaac agaatcaatc cgatgtcgcc gggtacgcca agcggatcgg gatgaggtat
      661 ggggagttgg ttctgcatac gcatgattcc tgggttagtt tttgatgctg gcggtcggtg
      721 caggcgccgg cgttgttgcg ggtgtgctat ccggtggagg ccatggaccc gcgcccacaa
      781 tatcgccgat atagtccgcc aatgcccggc gttcctgttc atcacccata aaaggcggca
      841 tgatgcccgt cgccggcagc gtcgttagac ggcgaaagat aaattcacga tcccagcctt
      901 tggccaaatc tttcatcgaa cgataaccgg tcggtgtatg acagctctgg cactgcgcga
      961 gaaatatttt ttgcccgacg gttttcatat tggccggcgt gatttctttt tcggcgatcc
     1021 atggataatg tttgagaata ccgtaattgg aagaatccgc cgaagctttt ttcaaggtgc
     1081 cgttggaata caaataaccg tacacggtgt acggcttacg aatcatttca cgaatactct
     1141 ccgtggcgcc ggtgacgatc aaacccagaa tcagtatcgc cacgctaaac ccgaaagtga
     1201 agattttcgg attgaaaaac gggccgacca atgcgatcac gtagatcgtc acggataaaa
     1261 gcatggtcag caaaagtacg cgggtcaaca cggaaagatt gcctgcgcca atattggcga
     1321 tgcccgtttc caaaagtttg gccacatcgg ccgggaggct tgtgaagtac cagtataaaa
     1381 agaacggcag caggaaaaac gaaggcatga gccacttggc cgaataatgt aataagcggg
     1441 tgcgtaaatt ttcgtctttt acgcgcgaag atgtaaaaat cgaaaacaca ccggcaatcg
     1501 tacacatgat caaaaaccga acgatgacgg acgggaaata tgaaggatta aaaaatccgt
     1561 cccacacatt accggtctca agccatttac cgggcgtcaa catgaacgtc agaataccat
     1621 taatcaccaa gagggaaaac gcggagatga taaaataaag ccaggctaac ttcacgtgaa
     1681 attgcggcga gactttgtcc caagagtagt aataaaaaaa cgcgatggcg atttcgacta
     1741 cgaagactac ccactcgatc gcccaaaaga acataaaatt atgaatcaaa gcctggatcg
     1801 aagcgggatt gaccaatccg acggcaaacc agataccgat gccgcccata gcgccaagta
     1861 cggtcgtgag caaaagcaaa aaattagaat ggcttcgaag ataaccgatc atagcttgat
     1921 cattgcgctt gaccgctaac gcttccgtga cggccatata caatccgcca ccgatagcaa
     1981 agtgggaaat atagacgtgc gtcatggcga tcaatccgat gatccatccg tgtccgacgc
     2041 cgcccacatt ccatacggga tattcaggca tatacgagcc ctttcattga aataaaattt
     2101 tcatttcgaa taatggcccc acatcgtgaa gatgataaag ccggtcaaaa atgcgatacc
     2161 aaaatagaat gtcgcccgtg aacgccatgc cggcgccgta cgcgaatcaa taaagggtat
     2221 cgcggcccaa agcacgccta ccaaactgaa gagcatgatt ccgaaaagtt cgccttcgat
     2281 cggtccgatt tccggcggga atagtttgag tagctggaac atggcgagga aataccattc
     2341 cggtttgata cctgccggag ccggcgctag aggatcggct tccgcgccga gttcccaggg
     2401 aaacatcacg gccagtaagc cgagaaaatt catcacgatc agccacacat atacgtcttt
     2461 gatggcaaaa tcgggaaaaa acttcatgta cttctttttg tcggcgggtt gattttcaaa
     2521 gaactccggt gcgtgaatgc cctgaagttg aacgaaaagc aaatgcaaac ccagtgtcat
     2581 cataaaaagc gccggcagca cccacacgtg cagcgcgtaa aagcgttcaa tggtttgcgt
     2641 gccgagttcc ttaccaccgc gcagcatgtc cgcgatgaac ccgcccatta ccgggacggc
     2701 ttctgtggtc ttcacgccga tctgtgtggc aaaataagcc aactgatccc acggcagaag
     2761 atacccgcta aatccgaagc cgaggcacat ggcgagcaaa atcatgcccg tcatccacgt
     2821 catttcccgc gggtatttgt aggatttcat aaaaaacaca ctgaacatat gcagaaatac
     2881 aaaaaagacc atgaggttgg ccgaccaact gtgtgtggag cgaatcaacc agccgaagtc
     2941 tacttgcgac atgatggctt gtacgctggc gtgggcttca ccggcattgt agtgaaccat
     3001 cagcagtacg ccggtcacga tctgaacgag aaataaaaat aatgagatgc cgcccatgta
     3061 ataccacaaa ctgtgcttgt gtacgggcac ttctttatgt ttgaaaaaag cgactatcca
     3121 gcctacgtcg tagcgttccc gtatgaattt gggaagaaac gcaatattcg gatcgtcatg
     3181 gtttttattg gccatttcgt tggacatagt ttttttgcgt ttagtgatgg attaaacccg
     3241 atgaatcacg atctcgtcgc cgttggtgac gactttgaac ttatctaaag gagccggcgg
     3301 cggcccggca atgttttttc cggtaagatc atagatgccg ccatggcatc cgcaaaaaat
     3361 atggtttttg tccgaagcgt attgcaccgt acacccgaga tgcttgcagg ttgcgccaag
     3421 cgccaccaat tcacccgcat cgtttttcag caagagtacg ggtttgcttc caaatttgat
     3481 caacttggcc cggactttgt caaagtcggc cactttaccg gcgacgaccg attgtacggc
     3541 attggccgta tcttcttcga cccgcggttt cagataacga tacaccgggt acaaaaatgc
     3601 gccgatccag agcataaagc cgcccgtaaa gaccgtacgc ataaaatccc gtttgtcggg
     3661 catatgaata tgctccgaag gatccttggt gtctttcata gacaggttct cgcgttaagt
     3721 tgtgaaacaa ttatgtagaa agattggact gattcagatc aaaagaagcg cgtcaatata
     3781 gataggcgca gcggatgacg caataatttt ttaggtgcat ggggcggtta aattaatttt
     3841 atttttctac ttgagatttt aaatataatt tatgactatt acgcgtccta caagcgacgc
     3901 gatgacactg cttgatataa tcggtttcac taaccaatca taaatagaaa gtttttccaa
     3961 tggatcgaaa gatttccttt gcaccggtac aaggcgcttt tagccaggta ctcaaggaac
     4021 gcgttcagca atactttgct gaaaaaggca tttccatgcg cggcggtttg catatggcta
     4081 tcaaaagcgg tttttatttg agtttggctg tgatgactta catccttata atttccggta
     4141 ttttatcggt atggggactt ttgcttgcag ccatgcttat gggcttttta gtagcctgta
     4201 tcggcttcaa tgtcgggcat gacgccattc atggcgcgat gtcgcatcgt aaatgggtca
     4261 acacgctgat ggggcttaca tttgaaattt tcggcgccag tgtgtatttg tggcgtatgt
     4321 atcacaatgt gattcaccac acctatacca atataccggg tgcggatcgt gatattgatg
     4381 gccgtccctt gataaaactc gcacccagcc agccgacgta taagattcac cgttttcaat
     4441 atatctacgc ttttgcagta tatacgctca catcgcttaa ctgggttctg cgtaaagact
     4501 atcagcgtat gtttatgaag cataacggtg tgtacacgac gaaaatgccg tcggtgccgc
     4561 tcatcgcaag catgttcatg ttcaagctcg tgcattttac gttatttctt attttaccca
     4621 ttattgtgtt gccggttccg ttttggatgg tgatcatagg atttttcgtg atgcacatga
     4681 cgacgggtat tacactggca ttagttttcc agatggcgca tgttgtagaa ggaatggagt
     4741 ttcccgaacc gcacgaagac ggtatgctcg aaaacgaatt ttttgtacat caattgaaga
     4801 ctacggctaa tttttcacgt aaaagtaaaa ttgctacatg gatttgcggc ggactcaatc
     4861 tgcaggtaga gcatcacttg ttcccgcata tttgtcatac gcattacccg gcgatttcgg
     4921 atatcgtaaa acgtaccgcg caagagtttg gaatcccgta ccacgaaaat acatcgttct
     4981 ggtcggcgct ggcatcgcat gttcgccatc tgcgtaaagt aggatacatc cctgtgcaat
     5041 tagggtaaaa tttggagata ggacttaaaa aaagtcatta cttttaaaat atttttctta
     5101 tcattcgccg caccaacatg cggcgaattt tttgcgtgtt ggctcagacg gatcgcataa
     5161 catcaaaaga ttattattat agttatagga gacaattacc gtaacactat gaatacgtat
     5221 ctcaaaaata tagtcgagta ttatcacgcg acggaaaaag cttaccatct gcactgggat
     5281 gtgaaaaaca gtttatccgt gcactacggt tattgggacg ataaagtctc ttcattttcc
     5341 gagtcgctgc ttcggatgaa cgaagtgatg gccgaagcgg tgggaattac atcatcggat
     5401 agggtacttg acgccggttg cggtgtaggc gggagcagta tttatcttgc caaaacccgc
     5461 ggctgccgtg cggtaggaat tacacttagt gataggcaag ttgcccaagc ggagactttt
     5521 gccaaagacc gtggtgtatc cgaacagacg gaatttaaaa tcatggatta ttgccatacg
     5581 gattttccgg atgcatcgtt tgacgtggta tggggatgtg aaagtatttg ttatgcggaa
     5641 tccaaagaac aatttatccg ggaggcatat cgtttgctta aaccgggagg gcgactggtg
     5701 attgcggatg gtatgacggc taagtttgag aacaatgatc atccgatcaa tcggcgctgg
     5761 ctagagggct gggcggctaa ctttatcgaa acaccggagc gcatgcaggg gtatatgcaa
     5821 cacatggggt ttacggatat tcgttttcgg gatatcacac cgcatgtcat gcattcctcg
     5881 cggagaatgt atatgctgta ctttccggcc aaggtctatc atgccgtagt acgaatgctc
     5941 gggcgcccgt cgccgttatt attcagaaaa aatgtggatg cctgccgttt tcaatacatt
     6001 gcattcaaac gaaaattgtg gatgtatggg cttctgtgcg gcgttaaaaa atagctgagg
     6061 agtatcatga gtaattacca cgatcagata gttgactatt atcgtgagac agaaaatcac
     6121 taccgtgtgt attgggcgct ggataaaaca ctggcgattc attacggata ttgggacgaa
     6181 aaagtaaaat catttccgca gtcccttctg cgcatgaatg aagtgctcgc cgaaaccgcg
     6241 aagatacaga aaacggatcg tgtactggat gccggctgtg gcgtcggcgg cagcagtatt
     6301 tttttagcgc agcatatcgg atgccatgcg acgggtatca cgttaagcgc taaacaggtt
     6361 gcacgggcgt ttgaaaatgc caaaaataaa aacgtcagcg atcgcacgga ttttaaggtg
     6421 atgaactatt gcaaaactga tttccccgat gcatcgtttg atgtagtctg gggatgtgaa
     6481 agcatttgtt atgcggatag taaagaacag tttgttcacg aggcgtttcg tttgctcaaa
     6541 ccgggcgggc gattgatatt ggcggacttc atggttccag cttttgaaaa taatcaggat
     6601 ccgagcgtac gccgctggct cgacggttgg gtcgtcaatt ttctcgaaac gccggataat
     6661 tttaaaaaat atatgctcga agccgggttt gaagatgtcc ggtttcgcga tatttccaca
     6721 cacacgcttc actccactaa acgcctcggg cgattgtatt attggggtaa ggtctatctg
     6781 gcgtattgca aaatcatcgg caaaacgatg gacccgattc gtttgaggaa tattaaatcg
     6841 gcttacgatc aataccaaag ccatcgtcgt gcattatgga tctacggtat gttttgtacg
     6901 acaaaaccgt cctgattgtg cttaactcgt ttatggcagg cgtattgatt aatcaaaaaa
     6961 ctagtttata ggtttaatcc atgagtgctt atcatcagaa tatcatcgca ttttatgacg
     7021 tacttgaaaa cccacttcgt atgatatggg atttagacaa gagttacgcc attcactacg
     7081 gctattggga cgaaaaagta aagtctttcc cggagtctct tctgcgcatg aatgaagtta
     7141 tggcggagac ggcgcaaatc aaatcccacg aacgcgtgtt ggatgccggc tgcggtgtcg
     7201 gtgggagcag catttttctt gcccgtacga tcggttgcaa aacagtgggt attacgttga
     7261 gtgccaatca agtacaacgc gctaagaaaa atgcgcaaaa ctccggcaca gcgcatttga
     7321 acgaattcaa agtgatggat tattcgcata cggactttga agatgcgagt tttgatgttg
     7381 tatggggatg tgaaagtatc tgttatgcag ataacaagga agagtttgtc aaagaggtct
     7441 atcggatttt aaagccgggc gggcgcttta tcatggcgga cggtatggta caaaaatttg
     7501 aagataatga taatcccatc gtggctaagg gacgcgatgg ttgggcggcc aactacgtag
     7561 agacaccgga acgcttcaaa caatttttag aaaacacggg ttttacaaat atcacctacc
     7621 gcgatattac ggatcatatc atgcattcgg ccaaacgcct ttattgggtt tcgttcccgg
     7681 cgaaattgaa tcagtggatt cgcccgttgt tcggaaaaac ggtggcggaa cataataaag
     7741 gaaatgtaaa tgcgtgcaag tatcaatatt tggcgttcaa aaaaaggctt ttagtgtatg
     7801 gaattttgtg tggcgtaaaa cgataagctg ttttttgtaa gctttcaaaa tgaaaattta
     7861 tgaattcgta tcaacaaaaa atcgttcaat attatcacgc aacggaaaaa tcatacaagg
     7921 atggatgggg attaaaaaaa aatctcatga tccattacgg ctattgggac gataaagtct
     7981 cttcattttc cgagtcgctg cttcggatga acgaagtgat ggccgaagcg gtgggaatta
     8041 catcatcgga tagggtactt gacgccggtt gcggtgtagg cgggagcagt atttatcttg
     8101 ccaaaacccg cggctgccgt gcggtaggaa ttacacttag tgataggcaa gttgcccaag
     8161 cggagacttt tgccaaagac cgtggtgtat ccgaacagac ggaatttaaa atcatggatt
     8221 attgccatac ggattttccg gatgcatcgt ttgacgtggt atggggatgt gaaagtattt
     8281 gttatgcgga atccaaagaa caatttatcc gggaggcata tcgtttgctt aaaccgggag
     8341 ggcgactggt gattgcggat ggtatgacgg ctaagtttga gaacaatgat catccgatca
     8401 atcggcgctg gctagagggc tgggcggcta actttatcga aacaccggag cgcatgcagg
     8461 ggtatatgca acacatgggg tttacggata ttcgttttcg ggatatcaca ccgcatgtca
     8521 tgcattcctc gcggcgtatg ttatattttt attttccgac acgctgttat catctgtatc
     8581 agtacctcat aagaaaaagt gtgccggaag tgaatcgcaa aaacattgat gcgctgttgt
     8641 atcagtatat cgccttcaaa aagaaattat ggctctacgg tatgttgagc ggagtgaagc
     8701 atcagtaaat attgtagtcc atacaaaaga ataatcggat gattgtatag atgaaaaaaa
     8761 tgaatgatcc gtaacagaat atttttttat gatatcggtt ttagatattc ccatccttcc
     8821 gagtgcaaat cttcaaatga aaatccgtct ttttcgttgc ccggcataat aaatacggca
     8881 tcggccatca cgtagttaaa atccagatac ggaacggctt cgatcaattt aggccaggtt
     8941 tcgcggagaa aatacgattc ttccggtata tctatcatac cttgaaagga ttcttgtgtc
     9001 ggtacatttt cgattaagaa ggcgagcgaa ataacgacgt attgtttttc tgcgctcatg
     9061 cgatactgtt cgtccatcgt ttcaccgact tcgccggact ccgttgccat actgctttcg
     9121 atttcgttga cccacgaatc aaattcggcc tgtgcttcgg ccgcatccgt cagataatcc
     9181 cagtacccat attcaaaaaa cggttcgttc tcaagtaaga gggctgcgac atttttaaaa
     9241 aatgcttttt tttcctctgg tgtttcacct tctttaacgg gcacgttgat cgccgcatgc
     9301 agcttggcac gggtttcatc attgataccg gtattgatga cgatctgaat gccgtaaaaa
     9361 tgaatcggac ccgagggttc atccgactta aaaatattat taaaaaaacc gcgacgattc
     9421 atgctttgta atccataaat tatgatgaag aactgctacg actgcggcca aacgatccgg
     9481 agcgacctga cgaagagcgg gacgatgaac ttttggacga cgagctccgg taactccgcg
     9541 aggaacgcga cgaagatgac gttgaggttg agcgcgacgc acgggatgtg ccggttgaac
     9601 tgtaattgcg tgactggcga acgcgggtga cattgccgtc ggaattaaca ttggcggata
     9661 cacggccaaa tccggtcggt ttttgtttgc ccacgccgct ggtttgactt ccgacggtac
     9721 gtgaagaaaa caatgtcgga cgacgttccg gtttgtattc cggtttcgac ggcccttgcg
     9781 aaggcgcacg ataaccgccc gggcgactga tcgtcgaagg cgtattatag ttaccaaaaa
     9841 atatcggcca ataggatgga ccgccgccgt aatgatatac ggttcgcgtg gatgccggtg
     9901 ttgcgacgac agtatctttt tgtgccggtg cggtcatggc tttggctatc gcggagtcgc
     9961 tccctgtgaa tgccgcgatc atcgaatcgg cggatgtttt agccgaggcc gatgtagcca
    10021 tggccgaagc gacggctggt ttgaaatccg acatgggcaa cagatccacg ctgatatttt
    10081 tttctttgta gtaaacaaag tcgtcattga gatcatccag ttcgtttttg gtgtcacgat
    10141 ccggtatgac atataaaacc cgattgactt tatacgacgc aagcgatggc gcggaaggca
    10201 ctttggccac gtaacgatta tcaaatacat cccattcatc ggtatattcg cccaagcgat
    10261 gattgtccaa taaaatcacg gccggcgctt cccacgaaag tttttcttcc gtttttacgc
    10321 ttacttcgcg tgcatagtac atcatcgcac cgagcgtgcg atgggccggt acgacgcctt
    10381 gcgggtgcgg ccagttatca aatccgatga ccacattggc gacgtcggct aaggccgcgc
    10441 cgaacgcaac ggattcttcc ccgtgcaaat cggcgatgat catcgtggat gaggggttgt
    10501 ccattttttg aatgatctcg cgcatgccga gtccgcgcgc ataggcggtt ttcatcgcgt
    10561 cggataatat cggcttcact tgtttacgta acgtttcctg acttagcgat tggactaacg
    10621 ttgtaacctt aaaaggtttc cagcgataat ttttgggttc ataggccacc atcaatgccg
    10681 ccggatcggc ataattttcc cacgcctgac cgaggctgtc acggctttgt tcgtctttga
    10741 agtcaagatt ttcgccgtcc gcgccgacct gccagccttg tttttgctgc aactcaagag
    10801 catcgagttc gacctcttca ccacagcccg taagaatgga tgcgaagggc agcattgcag
    10861 cgccggttgc gagggttgcc aatcgttggc cggcttcacg gcgtgaaatc atggcattcc
    10921 accatgcggg tgtttgggct ttttgattac gtttggtttt tctttttgcc atagtttatg
    10981 ttctagtgag aatgttggtc aatcaatgtc gctgcgctga gcgccgtacc gtccgtggcc
    11041 gtattggata aacgtgtata aataccggcc gcacgccctc cgacgagata cagtccgaaa
    11101 tttggaatta gattttcgcg gccgatgcgg attgacgaaa aataacgctg aaggatccag
    11161 tgtttgggaa tagctttgga cagcgattct ttccacagtg tttcactgac atcgttaccg
    11221 atgatcactt catcgccttc gcatccgtaa tcggatttga gtacccattc atttttattc
    11281 agttttttgc tttccttcgt tatcaggcgc cgcgtttccg ggatccacgt gcgtatcgta
    11341 ttccgcgcc
//
LOCUS       DAOQGF010000103        11271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2662_length_11271_cov_7.921541, whole genome shotgun sequence.
ACCESSION   DAOQGF010000103 DAOQGF010000000
VERSION     DAOQGF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_11271_cov_7.921541"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1369)
                     /locus_tag="PK937_12430"
     CDS             complement(230..1369)
                     /locus_tag="PK937_12430"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HNH33424.1"
                     /translation="MNRFFGIVYMVTLFGLSETVFSQTPANELMLRVDPRVELLCIAF
                     RLAEYPEVIDTLNSAYTQRIDAHFQKHKNHPLIEDIRLLLDSVKKHDIEFGYWDIPAI
                     AIHIRAFADWTSVAKADPPVDDIWDNRTWLTPRRMELIRAFYYDSDARRFFDDERDYY
                     ARAEASVNHRTMHLQKTWLQKYFGMAESEAYFAILGLQLRQGAYLRVNTPHEMRQTFT
                     LFPCTAFDHWGLPVSLDSLQIIRSILHEYIHAFVNQAVDAYESQWQKPAELILADPHV
                     WSLMKDAFYNNPRYLVYESLVRATSIIYLTRHHPELISLEKEITTQEKWGFYWMGEMV
                     RLLEDYDKERNRYSDFYSIMPEVCRLLESVSGRMRDGKYVFPNHQ"
     gene            complement(1407..4028)
                     /locus_tag="PK937_12435"
     CDS             complement(1407..4028)
                     /locus_tag="PK937_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH33425.1"
                     /translation="MIRSLIKKLWIGYVCLMWPATMTPLHTQDSATIITPERWARIHK
                     IDVQHIDLDLRLDWKTKSVEGYATIILQTRTVADRIELDGAFLTIHRIETGDGTPLRY
                     TYDGSDRDGALQIQLDRTLGTGEILTLTVFYKTGWVNYSDPNNLWGSNGKGIRFFEPT
                     FSEPRRRKQAWSISEYTSNRYWFPGHDTPSDLRTTTFSMRVDTPFTVIANGALLRTED
                     HPDGTRTFHWRMDTPYANHRTSFVAGRYSIFRQMTDGVVINNYSYPDEYDATVATVER
                     LPDMMRFYSEFTGQSYPFSQYAQVFVQDLPWGMENTTASTLTENMVDDHPTHTEFLFL
                     WDMLEGEALAMQWFGSYVPAASWEDIWLNRAFARYLSCMYDEYKNGRDEFLLWQLAFQ
                     HNTYLSDWASGIRLPVVARRTADEASLVTGNHTYLHGASVLHMLRKHVGDTAWKKILQ
                     HYVLTYGGRPVQTEDLRRSVEAVTGEPMDWFFDQWLYRTGHPVFTVTKEYHPGEKALT
                     LTVRQIQSPDTIASVFSQNRWFQGKMEIAVDDRIETIWIKPQSENVFRLACERAPRRV
                     IFDYEGTWIKEATFEKTFSEWIDQFTGDGDILARWDAMTRLSAMASDSGTSLSDKQNI
                     YAAFRTVIGGTAYWRLRYMATLQLQSLIAPPSNPKLVLDNETRRLLLTVIRRDSMWCK
                     TAAISLLGSTRDPSNASLFIDLLRDPKDRVVNAAAIALGKTKHSKAFDALSKLTRRPS
                     WKNQSLISALNGLRELGDARGADIAVAALSDRYAPRWTLVTPVWDFRIAAAQTLVALN
                     QSAKAYPIIVDRFQKAMEENDINDILNNALLIATLADPRGKEVFDALKAKFKNDANTM
                     TAVTQYENQFLEALKKK"
     gene            complement(4042..4629)
                     /locus_tag="PK937_12440"
     CDS             complement(4042..4629)
                     /locus_tag="PK937_12440"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNH33426.1"
                     /translation="MKKNVGILIFDDAEVLDFAGPFEVFAIASEVHDFSLYDVFTVAR
                     HMKPVRAVNGLLVTPNYDFSNHPPIDILIVSGGQGTRALLRDQEHHDWLQRVHRTTEY
                     TLSICSAARIFGALGLLDRKPYCTHHQVYDHLKEIVPTGQPQNDRRFVQTDERLYTSG
                     GISAGIDLSFHILEQRHGKEVAVRTAEYMEYRYTP"
     gene            4904..5827
                     /locus_tag="PK937_12445"
     CDS             4904..5827
                     /locus_tag="PK937_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNH33427.1"
                     /translation="MASQKIVFLILPEVHLLDLGGPEQVFLEAIGYGAPFEIEYCTYT
                     KELRSSGGLTFGHIKHFSEVAIKPEDYIFIPGPNVRSIHDYKFTSQTELLDWLRRCYR
                     ENITLCSVCSGAFALAYAGLLDYKKCTTHFKRTAELKRYFPKLDVQENVLFVEDGNII
                     TSAGIASGIDMALYILERIQGSHFAHKVAREMVIYTRRTGSQPQKSSYLDYRNHIHSG
                     IHKVQDYLSENIHKKSSVGHLAEIACMSERNFTRIFKKEAGITVNEYTTLVRKEKIKL
                     LLKNPDLSRVQIARAVGLNSDRQLSRIMHSI"
     gene            5927..6451
                     /locus_tag="PK937_12450"
     CDS             5927..6451
                     /locus_tag="PK937_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33428.1"
                     /translation="MDISVRRATIADATALALLARVTFEDAFGYVWTDKPVLKEYLDT
                     TFSVPKITSSLSKTNNSFWIALADGLPVGYAKMKKVSPYKLLEDNKPAQLQKIYMLRN
                     FIGQRIGERLQSALFDEVRMLGIRTLWLAVWDGNDKAIRFYERHGFQKTTRYAYDFHS
                     MHFEYEVMTKRFDV"
     gene            6448..6897
                     /locus_tag="PK937_12455"
     CDS             6448..6897
                     /locus_tag="PK937_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH33429.1"
                     /translation="MKWWAASVPFGLAFVAAIGNALVTYAQKKATPFDHPFYFGAFSL
                     LLASLGLFGIATFFSSGKIIPYAVENFVWFAVAAAGLILLNIFLYVLYRHYGAAYYTL
                     YAILAMVTTSIGLAVLVFKESMNVYFWLSLLFAALTVVCFIKGKSGG"
     gene            6971..7720
                     /locus_tag="PK937_12460"
     CDS             6971..7720
                     /locus_tag="PK937_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33430.1"
                     /translation="MFSNTIRGLLLWFIVLNWNAAVNAGDDSCRTKGSLRLRLHSSGF
                     FPYSGRLINRHPSGDIVGTYEKPFISAYVFQSLDLQTRNSDINYFQAGLYRWFHLSDT
                     WSVTPYAIIEIPQNGKWYNSPYAIGGASVLFSPHTSWQIENTTLWADGFKKADRADIV
                     HRLRLTYAPSHRWEWTGFVWANAATFDHQSFYSAAVMGSVSLFSEKHNPDIRLSVMLF
                     QMLRRDHGVGGKNRGIQCSLSIPWSYGSSAR"
     gene            8004..8474
                     /gene="tnpA"
                     /locus_tag="PK937_12465"
     CDS             8004..8474
                     /gene="tnpA"
                     /locus_tag="PK937_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNH33431.1"
                     /translation="MANTYTQLYIHIVSVVRGRMSLIPDNHKIELYKYMTGIIQNKGH
                     KLLAINGMPDHVHFLIGYDPHEALSALVKEVKRCSTNFINEKGWIKGRFSWQEGYGAF
                     SYAKSQLNTVCRYIENQEQHHKKQTFREEYVALLKRYEINYDERYVFSNVMEEY"
     gene            complement(8619..10256)
                     /locus_tag="PK937_12470"
     CDS             complement(8619..10256)
                     /locus_tag="PK937_12470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH33432.1"
                     /translation="MIKRIFKYFGIFFLLIIVAIGLIVGYVYVRHGGFFKYPSGGVLS
                     THQARYDVKSYDITLGVDPEEKFIRGTTTIKIESRADGLDTIQLNLIGLYDVLHVTGP
                     SQPRFTQQEDTLWVIMNQKLDSGSLADIGISYQGTPPTALYPPWFGGFNWSKDESGHH
                     WIGLSCQGEGGKIWFPCKDHPSDEPDSVALHITVPQPYVVAANGILKNITTTSHGMHT
                     YHWVTRYPINNYDINLSIGRYDIVERSYAAASGQTMPVQFYVLPQDRAKADTLIDMAV
                     DMLSTYRLYFGEYPFLSEKFGLVQTDYLGMEHQTINAYGNNFEYREFAGHRWDELMLH
                     EMGHEWWGNKVTVNDWADFWIHEGICSYGEALYQLSQTGEAGYHNHMSRLRKRIRNKK
                     PLVVHPNATTSEAYNDDIYSKGAYLMHSLRFMLGDSVFFKKLYRFANDSAYTYQNRVN
                     TNDFVRLWADVEDGNTTLFIREFIYTTNLPEITIDSTAAETYVIRSRNPKYAMPLEIQ
                     TSRGLERITVHTEGTRIQSGTTPVVDPRRWYLLKSEK"
     gene            complement(10377..10898)
                     /locus_tag="PK937_12475"
     CDS             complement(10377..10898)
                     /locus_tag="PK937_12475"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNH33433.1"
                     /translation="MLNAKLKPYAPYIGILLVLGILLMYSQMRQRKYEAKIEKVFDFA
                     ATEVTEFTVSKDDVKVTIAKGDSSWFFAAPDTGTADDYRVKQFIREVLGATREDAVSE
                     DTIKYNEYGVTESSAVKLELRANGKQLSKVYLGQSKKDIGQEYVRYAGDDRVYPLRGR
                     VISNLSAAASWWR"
     gene            complement(10891..>11271)
                     /locus_tag="PK937_12480"
     CDS             complement(10891..>11271)
                     /locus_tag="PK937_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33434.1"
                     /translation="VEGTFTSFFKDSARYASRAGFIPANNNGQIIIVTDNQFFNDRRA
                     GGVQENTDFILNAVDYLSGDKELIAIRSRDVSMRPLAEMTDSERRSWKWINILAPGAL
                     VILFGLYMWRRNRQQRKMLEDRYA"
BASE COUNT         2950 a         2534 c         2677 g         3110 t
ORIGIN      
        1 gcccagataa acttgagtac accctccacc gccaatccgg caattcgaaa cggcagcaat
       61 atcaaccata caaatgggta catgatcaag gcgaccaggg caagcggcca acacaagatc
      121 aacaaaatac accagataaa aaatgtagcc atagattttt tatctagtgt atacgtgaaa
      181 cctcatgccg ggtttcaatg gagaaacccg tcgtacatcc ggtgacagtt tattgatgat
      241 tagggaagac gtattttccg tctcgcatgc gaccggagac cgactccaat aaacgacaca
      301 cctccggcat gatcgaataa aaatctgaat aacgatttcg ttctttatcg taatcctcca
      361 gaagacggac catttccccc atccaataaa atccccattt ttcctgcgtt gtgatttcct
      421 tttcaagaga aatgagttcg ggatggtgcc gggtcagata gatgatcgat gtggcgcgaa
      481 ccaatgattc gtacacgaga taccgcgggt tattataaaa agcatctttc atcaatgacc
      541 acacatgcgg atcggctaaa atcagctccg ccggtttttg ccattggctt tcgtacgcgt
      601 ccacagcttg atttacaaac gcgtggatgt attcgtgtaa aatggagcgt ataatctgga
      661 gcgaatccag agacactggc agaccccaat gatcgaatgc cgtgcaggga aatagcgtaa
      721 aggtttgccg catttcatga ggtgtattta cacgcaaata ggcgccctga cgcaattgca
      781 aaccaagaat tgcgaaatac gcctccgatt cggccatgcc gaaatatttc tgtaaccacg
      841 ttttttgtaa atgcattgtg cgatggttca cgctcgcttc ggcacgtgcg taataatccc
      901 gttcatcgtc aaaaaatcgg cgtgcatcgc tgtcataata aaacgctctg atcagttcca
      961 ttcgccgggg tgtgagccag gtgcgattgt cccaaatatc gtcaacgggc ggatccgctt
     1021 tcgccacgga cgtccagtcc gcgaatgcac gtatatgaat cgcaatggcg gggatatccc
     1081 aataaccaaa ttcgatgtcg tgttttttaa cgctatccag gagaagccgg atatcttcga
     1141 tcaaaggatg gttcttgtgc ttttgaaaat gggcatctat ccgctgcgtg taggccgagt
     1201 ttaacgtgtc tattacttcc ggatattccg ccaagcgaaa agcaatacaa agtaattcaa
     1261 ctcgcggatc tacgcgcaac atcaattcat tggcgggtgt ttgagaaaac acggtttcac
     1321 tcaatccgaa taaggttacc atataaacaa ttccaaaaaa tcgattcatc accatctcct
     1381 gcatgtgtga ggatttatct caataatcat tttttcttaa gcgcttccaa aaattgattt
     1441 tcatattgcg taacggccgt catggtgttg gcatcatttt taaattttgc tttcaatgca
     1501 tcaaaaactt ctttgcctct gggatccgcc agtgttgcga tgagtagagc gttattaaga
     1561 atatcgttga tgtcgttttc ctccatcgct ttttggaaac gatctacaat aatcgggtaa
     1621 gccttagccg attggttcag tgcaaccaat gtttgtgcgg ccgcgatacg aaagtcccat
     1681 accggagtga ccagcgtcca acggggggca taccgatcac tcagtgctgc gaccgcgata
     1741 tcggctccgc gcgcatcccc caattctctc aaaccattga gcgcactgat caggctttgg
     1801 tttttccatg aagggcgacg ggtcaattta ctcaaagcgt caaacgcttt agagtgtttg
     1861 gttttgccca aggcgatggc ggctgcgttt acgacacgat ctttaggatc gcggagcaaa
     1921 tcaataaaga gcgaagcatt cgatggatcc cgggtagagc ctaataagct tatggctgcg
     1981 gttttgcacc acatgctgtc cctgcgaatg acggtaagaa gtaagcggcg cgtttcatta
     2041 tcaagtacca gtttaggatt cgaaggcggc gctatcagtg attgaagttg aagcgtagcc
     2101 atgtaacgga gtcgccaata tgccgtacct ccgatcacag tgcgaaaagc agcataaatg
     2161 ttttgtttat cgctaagcga tgtgccggag tctgaggcca tcgcggaaag tcgggtcatc
     2221 gcatcccatc gggctaaaat atcaccgtcg ccggtaaact gatcaatcca ctctgaaaat
     2281 gttttttcaa atgttgcctc tttgatccac gtcccttcgt aatcaaaaat aacacggcgc
     2341 ggcgcgcgtt cgcatgccaa acgaaataca ttttccgatt gcggtttgat ccatattgtt
     2401 tcgatgcggt catctacggc tatttccatc tttccctgaa accagcggtt ctgtgaaaac
     2461 accgacgcga ttgtatccgg tgattggatt tgacggactg taagcgtcag ggccttttcc
     2521 ccgggatgat attctttggt tacagtaaac accgggtgac cggttcgata cagccattga
     2581 tcaaaaaacc aatccatcgg ttcaccggtg acggcttcga cagatcggcg caaatcctcc
     2641 gtttgaacgg gacggccgcc gtaagtcagt acatagtgtt gcaggatttt tttccacgcg
     2701 gtgtcgccga catgcttgcg aagcatatgc aaaacactgg cgccgtgaag ataagtgtga
     2761 ttaccggtca ccagagaagc ttcatctgcg gtgcgtcggg caactacagg aagccgtata
     2821 ccggatgccc aatcggagag gtacgtgtta tgttgaaatg ccaattgcca caggagaaat
     2881 tcatcgcgac catttttata ttcgtcgtac atacaagaca aataccgcgc aaaggcgcga
     2941 ttaagccaaa tatcttccca tgatgcggca ggaacgtaac ttccaaacca ttgcatcgcc
     3001 agcgcttcac cttccagcat gtcccataaa aaaagaaact ccgtatgcgt cggatgatca
     3061 tccaccatgt tttcggttag tgtggaagcc gttgtatttt ccatacccca aggcaaatcc
     3121 tgaacaaaaa cctgagcgta ttgtgaaaag gggtacgatt gcccggtaaa ttcggaataa
     3181 aaacgcatca tatcgggtaa acgttcgacc gtggctacgg tagcgtcgta ctcgtcggga
     3241 taactatagt tgttgatcac gacaccgtcg gtcatctggc gaaatatcga ataacggccg
     3301 gcgacaaagg atgtacgatg attggcatac ggcgtatcca ttcgccagtg aaatgttctg
     3361 gtgccgtcgg gatgatcttc cgtgcgcagc agagcgccgt tagcgatgac cgtaaacggt
     3421 gtgtccacac gcatcgaaaa tgtggtggta cgtaaatccg aaggcgtatc atggccggga
     3481 aaccaataac ggttagaagt gtattcggaa atactccagg cttgtttgcg gcggcggggt
     3541 tctgaaaatg tgggttcaaa aaaacgaatg cctttgccgt tgcttcccca gaggttattc
     3601 ggatcactat agttgaccca tccggttttg taaaaaacag tcaaggtgag gatttcacct
     3661 gtgccaagag tccgatccag ttgtatttgc agagcgccat cgcgatcact gccgtcgtac
     3721 gtataacgca aaggcgtccc atcgccggtt tcgatacgat gtatcgtcag aaaagcgccg
     3781 tcgagttcga tacgatccgc gacggtacgc gtttgcaaaa tgatagtcgc atatccttcg
     3841 acggattttg ttttccagtc cagacgcaga tccaaatcga tatgctgcac atctattttg
     3901 tgaatgcgcg cccaacgttc cggcgtgata atcgttgcac tatcctgcgt gtgcagtggt
     3961 gtcatcgtcg caggccacat caggcataca tatccaatcc ataatttttt aattaatgac
     4021 ctgatcatac caaactccgg tttatggtgt ataacggtat tccatatatt cagctgtccg
     4081 aacggcgact tctttgccat gacgctgctc aagaatgtga aatgaaagat ctatgcctgc
     4141 ggaaatgccg cctgacgtgt aaagccgttc atcggtttgt acaaagcgcc gatcgttttg
     4201 cggttgtccg gtgggtacga tttcttttaa gtgatcgtac acctgatggt gcgtgcaata
     4261 gggtttacga tcgagcaaac cgagtgcgcc gaagattcgt gcggcggaac aaatgctcag
     4321 agtgtattcg gttgtacgat ggacgcgttg taaccaatcg tggtgctctt gatcgcggag
     4381 taacgctctc gtgccttgcc cgccggagac gatcagaata tcaatcggag gatgatttga
     4441 aaaatcataa ttgggtgtga cgagtaaacc gttgacggcg cgtacaggct tcatatgccg
     4501 ggcgaccgta aatacgtcat acaaggaaaa atcatgcact tcggatgcaa tagcaaaaac
     4561 ttcaaacggc ccggcgaagt ccaatacttc cgcatcatca aaaatcaaaa tacctacgtt
     4621 ttttttcata atacctcgtg cgaaaatcgg tgacgtgtat acttttttac aaaatacggc
     4681 tcatgaaaaa atttgcatcg ccaataaaaa gacatttctg gccaataatt ttttgaaata
     4741 aattaagctg gcttgtgttt ttatttttta cagttatcta atttgatttt attgggttta
     4801 tattgctggg taggttaata ctgattggcc gaaaatgtct gattaatggc gcacgggtta
     4861 gcataaccgg tgttatcttt aaaccaagat caaaggacgt gttatggcat cacaaaaaat
     4921 tgtttttcta atattgccgg aagttcattt gttggatttg ggcgggcctg agcaggtgtt
     4981 tttggaagct atcggttacg gtgcgccgtt tgaaatcgaa tactgtacat ataccaaaga
     5041 actgcgaagt tcgggcgggc tcacgtttgg tcatatcaag cattttagcg aggtggcgat
     5101 caaaccggag gattatattt ttatacccgg tccgaatgtc cgatccattc acgattacaa
     5161 gtttacttca caaaccgagt tattggattg gttgcggcgt tgctatcgtg aaaatatcac
     5221 gctgtgtagc gtgtgctccg gcgcatttgc gctggcttat gcaggtttat tggattacaa
     5281 aaaatgtacg acgcatttca agcggacggc tgaattgaaa cgttattttc caaaactgga
     5341 tgtacaggaa aatgtgcttt ttgtagaaga cggtaatatc ataaccagtg cggggattgc
     5401 ctccggtatt gatatggcgt tatatatttt agaacgcata cagggatcgc attttgcgca
     5461 caaagtcgcc cgtgaaatgg tgatctatac acggcgcacg ggttctcagc cccaaaaaag
     5521 ttcgtacctt gattaccgaa atcacattca ttccggtatc cataaagtgc aggattatct
     5581 ttcggaaaat attcataaaa aatcttccgt cggtcatttg gccgaaatcg cgtgcatgag
     5641 tgaacgtaat tttacacgca tttttaaaaa agaagccggg atcaccgtca atgagtacac
     5701 gacactcgtg cgtaaagaaa aaataaaatt gttacttaaa aacccggatt tgtcgcgggt
     5761 tcagattgcc cgtgcggtgg gattgaatag cgatcgtcag ttgagccgaa tcatgcattc
     5821 gatctaatcg gataagtatg aatgaaaaat gtttgatata aaaaaactgc gtcgagtgaa
     5881 aataaacaat gaaatcacga cccatatttg aactgaggaa atgagcatgg atatatcggt
     5941 tcgtagagcc acgatcgccg atgcgacagc gttggcactt cttgcgcggg ttacatttga
     6001 ggatgctttc ggttacgtgt ggaccgataa accggtactg aaagaatatc tcgacacaac
     6061 tttttccgtt cccaaaatca catcgagtct cagcaaaaca aacaatagct tttggatcgc
     6121 tctggccgat ggtttgccgg taggttacgc caaaatgaaa aaagtgtcgc cgtacaaatt
     6181 actggaagat aacaaacccg ctcaactgca aaaaatatat atgctgcgca attttattgg
     6241 ccagcgtatc ggagagcgac tacagagtgc tttatttgac gaggtgcgca tgttgggtat
     6301 tcgtacgctt tggctcgccg tctgggatgg taatgacaag gccattcggt tttacgaacg
     6361 tcatggtttc caaaaaacga cacgctatgc gtatgatttt cattcaatgc attttgagta
     6421 tgaggttatg accaagcggt ttgacgtatg aagtggtggg cggcttccgt gccgtttgga
     6481 ttggcgttcg tcgcggcgat aggcaatgcc ttggtcacct atgctcaaaa gaaagcgaca
     6541 ccatttgatc acccgtttta ttttggtgct ttttcattgc ttctcgcatc gctcggattg
     6601 tttggcatcg caacattttt ttcttccggt aaaataatac cctacgccgt ggaaaatttt
     6661 gtctggtttg ccgttgccgc agcaggtctg attttactca atattttttt gtacgtgctg
     6721 taccgtcatt acggagccgc ctattatacg ctgtacgcta tactagcgat ggtcactaca
     6781 tccatcggcc ttgcggtgct ggttttcaaa gagtctatga atgtgtactt ctggttatcg
     6841 ttgttatttg ccgcgttgac cgtggtgtgt tttatcaaag gaaaatcggg gggctaattt
     6901 gcgcaagaat cggattggaa aacgcaacgg ccggtcataa atttttcaaa aaaacaaagg
     6961 agacataaac gtgttttcaa atacgatccg tggtttgttg ttatggttta ttgttttgaa
     7021 ttggaatgcc gcggtaaatg cgggagatga ttcatgccgc accaaaggtt cgcttcgttt
     7081 gcggttacat tcatcggggt tttttccgta ttccgggcga ttaatcaatc gtcatccgtc
     7141 gggtgatatc gtcggcacgt atgaaaagcc gtttatttcc gcgtatgttt ttcagtcgtt
     7201 agacctccaa acgagaaatt cggatatcaa ctattttcaa gccggtctgt atcgctggtt
     7261 tcatcttagc gatacatggt ccgtgactcc gtacgcgatc atcgaaattc cgcaaaatgg
     7321 gaagtggtat aattcgcctt acgccatcgg cggtgcgtcg gttttatttt cgccgcacac
     7381 ttcgtggcag atagaaaata cgacgctgtg ggccgatgga tttaaaaaag cggatcgcgc
     7441 ggatattgtt catcgtctgc gcctcacgta tgcaccgtcg caccggtggg aatggaccgg
     7501 atttgtgtgg gctaatgcgg cgactttcga tcaccaaagt ttttattcgg ccgcagtgat
     7561 gggcagtgtt tcactttttt ccgaaaagca taatccggat atacgtttgt cggttatgtt
     7621 atttcagatg cttcggcgtg atcacggtgt cggcggaaaa aatcggggca tccaatgttc
     7681 gctgagtatt ccgtggtcgt acggttcctc ggctcgttaa aatgaaacat cgtatgccta
     7741 cgggtgtttt tagttattca caggttttgt tgttttcgct attcagtaaa tattgctaac
     7801 gcttgaatgc aaattttccc tgatctatat ttttcttaaa tcgttactta ttttttctat
     7861 tagtcgcttt gtaagttctg gcaacgctct ctatgtctca ggagccattg atcagattgt
     7921 attcccgtta gggaataaaa atggttaact atgaactgat agaaagcgtg ccattcctgt
     7981 agggaatgaa ttagaggaaa actatggcta acacctacac acaactttac atccacatcg
     8041 tttcggtcgt cagaggtcga atgagtttga taccggataa tcataagatt gagctgtata
     8101 aatacatgac cggcattatt caaaacaaag gccataaact ccttgcgatc aatggcatgc
     8161 cggatcatgt tcattttctg ataggatacg atccccacga agctttatcg gctctagtca
     8221 aagaggtcaa acgatgttcg accaatttca taaacgaaaa aggttggatc aaaggccgat
     8281 tttcttggca ggaagggtat ggtgcgtttt cctatgccaa gtcgcaatta aataccgtct
     8341 gtcgttatat tgagaatcaa gaacagcatc acaaaaaaca aacattccgc gaagaatacg
     8401 tcgcgttgct taagcggtat gagattaact acgatgagcg ttatgtcttt tctaatgtta
     8461 tggaagagta ttgaaaccct acgggtttca tctttccttt gttgaggcat taccaaattt
     8521 gaaagcctaa cggctttctg agcgtcatct tttgaactaa agcaattcaa gaaacgacaa
     8581 attctttgtt cgtccttctt agatctataa tccgatgatc acttttcact tttaagtaaa
     8641 taccaccgtc tcggatccac taccggtgta gtgccgcttt gaattctagt cccctcggtg
     8701 tgaactgtga tacgctccaa tccacggctt gtttgtattt cgagaggcat cgcatacttc
     8761 gggtttcggc ttcgtatgac atacgtctcc gcagctgtcg aatctatcgt aatttccgga
     8821 agattggtag tgtagataaa ctcgcgaatg aataatgttg tgttgccatc ttctacgtct
     8881 gcccaaagcc gaacaaagtc gttagtattg acacggtttt gataggtgta agcgctatcg
     8941 ttggcaaagc ggtatagttt tttaaaaaac accgaatccc cgagcataaa acgcagcgaa
     9001 tgcataagat aggcgccttt ggagtagatg tcgtcgttat atgcttcgga cgtggtcgca
     9061 ttgggatgaa cgacgagcgg ctttttattg cgaatccgtt tacgcaaccg cgacatgtga
     9121 ttgtgataac ctgcttctcc ggtttgggac agttgataca aggcttcacc gtaggaacaa
     9181 atcccctcgt gaatccaaaa gtcagcccaa tcgttgactg ttactttatt gccccaccat
     9241 tcatgcccca tttcgtgcaa catgagttca tcccatcggt gtccggcaaa ttcccggtat
     9301 tcgaaattat tgccgtatgc attgatcgtc tgatgttcca tcccgaggta atcggtttgt
     9361 actaaaccaa atttttccga cagaaacgga tattcgccga aatacaaccg gtaagtgctc
     9421 agcatatcaa cagccatgtc aatcaaagta tccgctttcg cacgatcctg aggcaacaca
     9481 taaaactgga cgggcatcgt ctgtccggac gccgctgcat acgaacgttc tacgatgtcg
     9541 tatcgtccga tgctcagatt gatgtcgtaa ttattgatgg ggtagcgcgt cacccaatga
     9601 tacgtatgca ttccgtgcga tgtggtagtg atgtttttca aaatgccgtt ggcggcgacg
     9661 acataaggtt ggggaacggt gatatgtagc gctacgctgt cgggttcgtc cgaaggatga
     9721 tctttgcacg gaaaccatat tttaccgcct tcgccttgac atgaaagacc gatccaatgg
     9781 tgaccggatt catcttttga ccaattaaaa ccaccaaacc acggtggata caatgcggtg
     9841 ggtggtgtac cttgatatga aatgccgata tcggctaagg agcccgaatc aagtttttgg
     9901 ttcatgatga cccagagcgt gtcttcttgt tgtgtgaagc gaggttggct cggtccggtg
     9961 acatgaagca cgtcgtataa cccgatcaga ttcagttgga tggtatccaa tccatcggcg
    10021 cgggattcga tttttatcgt cgtagttccg cgaataaatt tctcctcagg atcaacacct
    10081 agtgtaatat cgtacgattt gacatcgtac cgtgcctgat gagtcgacag gacaccgccg
    10141 gacgggtatt tgaaaaaacc accgtgccgg acatagacat acccgacgat caatccgatc
    10201 gcgacgatga ttaagagaaa aaaaataccg aaatatttga atatccgttt gatcatggga
    10261 aaaactccga tggagtccgc tccgtgtaac ttacgaagta tttccattct gtaaaaataa
    10321 aaaaagcgga gaagttggtt tctccgcttt gagatatacg aaaatctgat ttcctcttac
    10381 cgccaccatg aagccgcagc gctgaggttg ctgatcacgc gtccgcgcag cggatacacc
    10441 cgatcatcgc cggcatagcg cacgtattcc tgaccgatat cttttttgga ttggccgaga
    10501 tatactttgg aaagttgttt tccgttggca cgcagttcga gtttgacggc ggaactttcg
    10561 gtgacaccgt attcattata cttgatcgta tcctcgctca cggcatcttc gcgcgtggca
    10621 ccgagcactt cgcggataaa ttgtttgacc cggtaatcat ccgccgtacc cgtatccggc
    10681 gcggcaaaaa accatgacga atcgccttta gcgatggtga ctttgacgtc atctttgctg
    10741 acggtaaatt ctgtcacttc cgtggcggca aaatcgaata ccttttcgat tttggcttca
    10801 tacttacgct ggcgcatctg cgaatacatc agtagtatgc ccagcaccag cagaatgccg
    10861 atgtacggcg cgtacggttt gagtttggca ttaagcataa cgatcctcca gcattttgcg
    10921 ttgttggcga ttgcggcgcc acatatacaa accaaaaagg ataaccagcg ctccgggcgc
    10981 gaggatattg atccatttcc aggagcggcg ttccgaatcg gtcatctccg caagcgggcg
    11041 catcgagacg tcgcgtgaac ggatggcgat caattctttg tcgccgctca gataatccac
    11101 cgcattgagt ataaaatccg tgttttcctg cacaccgccg gcgcggcgat cgttgaaaaa
    11161 ctgattgtcg gtgacaatga tgatttggcc gttattattg gcggggataa atcccgcgcg
    11221 gcttgcatac cgtgcactgt ctttgaaaaa gctggtaaac gttccttcaa c
//
LOCUS       DAOQGF010000104        11198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2692_length_11198_cov_5.411828, whole genome shotgun sequence.
ACCESSION   DAOQGF010000104 DAOQGF010000000
VERSION     DAOQGF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2692_length_11198_cov_5.411828"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK937_12485"
     CDS             <1..220
                     /locus_tag="PK937_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNH33435.1"
                     /translation="PAEMVSAVSEYVNIPVIVGGGIRTPEDALQKVEAGASFVVTGNV
                     LEHSGSLSLIREFANAIHWKTPAAQKHA"
     gene            226..942
                     /locus_tag="PK937_12490"
     CDS             226..942
                     /locus_tag="PK937_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNH33436.1"
                     /translation="MGIRRRALLVFAKLPEPGKVKTRLIPALGKDGACRLYQAFLKDS
                     IIQYTRLALEVGYTPYLFVTPSEAVSFFENSCDTLTSEIGTNNAWQVRLQSQGDLGAR
                     MKDAFAGLFAQNHDACWIIGTDHPTLPDAYVVDAFRYLDQYDAVIGPADDGGYYGLGL
                     QRRMDFLFEDIPWSTEQVLSTTKLRMQNNRVNYYPIKPWYDVDEPADMYRLKRDLDKA
                     GMRCNYTKQWFEQHGMTYGR"
     gene            932..1405
                     /locus_tag="PK937_12495"
     CDS             932..1405
                     /locus_tag="PK937_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH33437.1"
                     /translation="MADKQKNLKFLHFRSGNSDYAVELEYVKEIIRSVAVSPMNELPP
                     FAPGVINLRGEIIPIIDFLLRSGAGATTLRLKSRIIITRIHDITAGLLVDDVLETLEV
                     EPSDITRNLHADVLIDVKYIQGTFLSGGTTIICVDLNKLMTDKEYAVLRHEVKHA"
     gene            1398..3101
                     /locus_tag="PK937_12500"
     CDS             1398..3101
                     /locus_tag="PK937_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNH33438.1"
                     /translation="MLNYHEVHPMGTGHGVIAKEADFTDQDFIRFRNLIRARSGIYFN
                     DRKRADLKSGVLKAFHYSGVSGLDKYFDLLASDPTQSAQFKTLVSFLTVGETYFFRHF
                     DVIEKVVLPSLIKAHHHDKTIRIWSAGCSTGEEPYTVAMVLHNLLPDIRDWKISIVGT
                     DINVNSVEYAKEGVYRPWSLRSITDFYKNFYFDKKDGLYYIKDFIRNMVRFDYLNLVD
                     DGYPRDENGTKDLDLIFCRNVTIYFEAETTIKVVNRFYDCLKEKSFLAVGHAEPSSLV
                     YDKYISEIYPDAVIYRKDTALKKEQQYKTGIRTRRDIFKEFKDQTSVVKNQGIINNLD
                     TLQQRITKLDITDTVKKENPVLKSEPKPREPLFKADNLTITTMRAPLKPQPGAKPNDR
                     DAVIEEKRRAELNEAELFTMALEEFYKKDFREAEKHFTALIEKNPANGRALYMIAHIN
                     ANLDNVSKAKDFCHMAIAEDSLMLEAYYLLGLIYKEENLFDESVRMLKKTIYIDSDFA
                     IGYYELAVNYFKLGDGVQARKYLKQTERILKNKSDDERVGILDDLKVKELRMMVNMWD
                     N"
     gene            3124..3714
                     /locus_tag="PK937_12505"
     CDS             3124..3714
                     /locus_tag="PK937_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNH33439.1"
                     /translation="MKQQDVFDEVHKLMAELLSSRLTPEAEAKILAGRAEKLKRAHTN
                     EETGEQISVLIFQLSSEFFALETQYVSEVRVLSEVTPVPCTPDFVLGITNIRGAVFSV
                     IDIRGSFNLEDRPVTDRSMFLMIQWKGIELCLLADAVIEKINLPRSEVKTMTGGNREH
                     MSSFVSGFFIRNDNKITLISWDNYVSHSNIIVNEEV"
     gene            3733..4959
                     /locus_tag="PK937_12510"
     CDS             3733..4959
                     /locus_tag="PK937_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH33440.1"
                     /translation="MIFLFFLLMAIPAAAIGTFVVYMVYGRKKFILSVLIRTVPWLVL
                     IVCAAFFIGIYSMHWWGWVIGLGAAVIFGVIYYLVLFRSTFTVTMDIVDVVTRIADKD
                     YSKRLTFKADGELNTMRESLNSVIDNLENTEKTLGDAVSRLSSVVSELNSTMDQQVVG
                     MNEQSTSLNQTTTTTQELAATSHQTTEKAQFVVESTERSMDITSKGKDAVDNTVDEMN
                     EIKKRVERIAEQILDLSEKTQQIGVITTTVNDIAEQTNMLALNAAIEASKAGEFGKGF
                     GVVAFEVRKLAEKSKEASLRIRDLITQIQNATNSTVMATEEGSKRVDIGVEKIREAGK
                     YMDESIQSLEESVGYAQQILVGSKQQTIGIEQITLAMANINEVVKQVAKGTAQTQGAV
                     DSVLGLTKEMDSLVKK"
     gene            4999..7455
                     /locus_tag="PK937_12515"
     CDS             4999..7455
                     /locus_tag="PK937_12515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNH33441.1"
                     /translation="MDLSKDELKEMMSIFKVESEEHLKNLNKGLLKLEETPNSRELID
                     ELFRTAHSIKGSARMMGFQKIEGVSHKIEDIFGLVRRAEIKVSPENFDVIYEGVDIIS
                     QIIELISNEGSDDSIDISVIVKKLEAIYNPQEAPASVAESKEESKPDVEPVLDVVSKA
                     EPVPVTEAKTPEPEETEFKPTTDLSKSEVSVDKTDAAKGNVSEDAFIRVPTRRLDDLM
                     NQVSELVTTRIKSQQRLADIRKMLDFTEEWAQQTERIRLLLDRTLSRIVNEDVERPSD
                     VKSELITSYDLRTVVTSYLSSIEKIGSIAESISILYDKQSEENLRMSVITGDIQDNLR
                     IIRLLPISTIFDLYPRMIRDIAKLQSKKVKFEVFGGDTRVDKKVLEELKDPLIHLLRN
                     SIDHGIEAPADREATGKAQEGTIQVRAGYVGNMVQIEVQDDGRGIDTDRILQIAIKKG
                     YIKEHQASEVSKNDLVGLIFHSGFSTAKIITDLSGRGVGLDIVKSNIEKIKGTIETVS
                     EKGKGTRFTIKIPLTLATTHVLIVDVGGDHYSLPIDFIDRTVRLTEEELWTGGQNPFV
                     LVDNTPVPLYKMEEVLGNAKQRLKNKGLLKSSAAPKIGQHLTKIETNKFPAVIFVSGG
                     RRVAFLVDKLLDEQEVVVKSLGHQLKRVKNVAGSTVLGDGRISIILDPNDLIKSVQGS
                     GTKFAFRDRRQRDLIKKRVLVVDDSITTRTLEKNILESAGYHVTIATNGLEGYQKLHE
                     IGNFDIVVSDVEMPFMTGFEFAHKVRTESKFPDIPFVLCTSLESDKDKRRGIEVGANA
                     YIVKGGFDQTNLLETIEKLL"
     gene            7462..8634
                     /gene="cheB"
                     /locus_tag="PK937_12520"
     CDS             7462..8634
                     /gene="cheB"
                     /locus_tag="PK937_12520"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNH33442.1"
                     /translation="MPKILIAEDSPTVTMILQKIFATDDDCQVIGVAKNGREAVEKVQ
                     ILRPDIVTMDIRMPVMDGFEATKQIMQTSPTPILVISASVGKDDLNIAFNAIRAGALD
                     IVEKPKGNLAMDYEYIGRDLIKKVKILSGVKVFHHIGGAKNKSSDAKSNDKTDTLSVR
                     QPRVPAPAAEVPSVKETLWREPLTPKSVYPKLLSPVKTPEVIAIASSTGGPSALLKLL
                     KTLPAGFPVPVVIVQHICEGFGQGFVEWLNKECGMNVKTADRGEELQPGTIYVAPDGY
                     HLFIDSGKIVRLSKSMPVNGLRPNATLMMESVAKVFGAASIGIVLTGMGRDGADGAKA
                     IKEAGGFTMAQNAESCVVFGMPKETIELGVVDKILPPEKMTAELMAFFKLYQTANA"
     gene            8644..10332
                     /locus_tag="PK937_12525"
     CDS             8644..10332
                     /locus_tag="PK937_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33443.1"
                     /translation="MAKEKILVVDDSATQLIMYKMALSKAGYLVISAKNGVEGMHMVE
                     TENPDLIVSDIIMPELNGYQFCRLVKDDPSRAHIPIILLTSLGQQQDRFWGIEAGANA
                     FVTKATDTTALLQAVEELIKTIAKPSTIDYADKEDEKYSIQSPENPEESIKSKLNRIL
                     EKLLYETTVSNRMRDLSRYAYNRQEMITRCFDLFRQIVEYHASALTLFEKDTLHLTID
                     IQKDIADNFLDKAIASVLQQDFNPNEVRMGLIKNTQKDIFGETHLKPEGAQDVQSVLV
                     VPLRDETEVIGSLAVFTSDTFAYNKDIYRLIQLIARELTLTIKYIWKLEELENIKRNF
                     TSTIVNDLRSPIIANQSFAEALFNEYVDPITEDQREILSNVIANNKKLLTFINDIIDI
                     SKIESGKLEIFPEHNNVHTIIEDAIKNMSVLASQKEILLINEFENTDYKAYFDGEKIL
                     QVFNNLISNAIKFTSTGGAVMIGAQPVPDGVQFMVKDTGIGIPEEDLPHIFEKYKRTG
                     GVFSSDDHAGGGGIGLGLAICKSIIEAHKGRIWAESAVGEGTTFYFTVLTPEQS"
     gene            10336..11094
                     /locus_tag="PK937_12530"
     CDS             10336..11094
                     /locus_tag="PK937_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33444.1"
                     /translation="MLDDIFTPSDEQDITLTNDERTRIEGILSKLVTESAQQGVSIVA
                     LKNHKISKPILFKLAKDGNSNASYVLSELNLRGILEEAKNPDTREEVATEMLARLVVC
                     PVPSIRRRAIHLLLKINPEKFSEVINERNLSPNLKNEIFSDVIADEEAGLRIMLRMLK
                     NPGTPQDIKDKVIAKIGRIRKMIEENSAQIIDWNHRIEDLEKRKRIKFHMVEKYYAEV
                     QQSLDEFKNLPNFVMDLKFDVDLAFENLKHTIKE"
BASE COUNT         3269 a         2305 c         2703 g         2921 t
ORIGIN      
        1 gccggcagaa atggtttctg cggtcagtga gtatgtgaat ataccggtga tcgttggcgg
       61 gggtattcgt acacccgaag acgcccttca aaaagtggag gccggcgctt cgtttgttgt
      121 gaccggtaat gtgctggaac actccggaag cctgtcattg attcgtgaat ttgcgaatgc
      181 aatacactgg aaaactccgg cggctcaaaa acacgcgtga gtaatgtggg catacgacgg
      241 cgtgcattgc tggtgtttgc caaattaccc gagccgggca aagtaaaaac tcgattgata
      301 ccggcccttg gaaaagacgg ggcctgccgt ttatatcagg cttttttgaa agacagcatt
      361 attcaatata ccagacttgc tttggaagta ggttatacac cgtatttatt tgttacgccg
      421 tcggaagcgg tttcgttttt tgaaaattct tgtgatacgc taacttcgga aattgggacg
      481 aacaacgcat ggcaagtgcg attgcaatca caaggggatt tgggcgcccg tatgaaagac
      541 gcgttcgccg gtctttttgc tcaaaaccat gatgcatgct ggattatcgg gaccgatcac
      601 ccgacgttgc cggatgctta tgtggtagat gcttttcgtt acttggatca atatgatgct
      661 gtcatcggcc ctgccgacga tggcggttac tatggtttgg gattgcagcg aagaatggat
      721 tttttatttg aagatatacc ttggagcacg gaacaagttt tgtccacaac taagctacgg
      781 atgcagaaca atagagtgaa ttattacccg atcaagccgt ggtatgatgt ggatgagccg
      841 gcggatatgt atcgcctcaa acgtgattta gacaaggccg gtatgcgatg taattatacg
      901 aagcaatggt ttgaacaaca tgggatgaca tatggccgat aaacaaaaaa atctgaaatt
      961 ccttcacttt cgatccggca acagcgacta tgccgttgaa ctggaatacg tgaaagaaat
     1021 tatacgcagc gttgccgttt ctccgatgaa cgagctgccc ccgtttgcgc cgggcgtcat
     1081 caatctccgg ggagaaatca taccgattat tgatttcctg ttgcgttcgg gcgccggtgc
     1141 gacgacgttg cgtctgaaat cgcgcatcat cattacgcgg attcatgata ttacggccgg
     1201 tctgcttgtt gatgatgttt tggaaaccct tgaagttgaa ccatcggata ttacacgcaa
     1261 tttgcacgcc gatgtcctca ttgacgtcaa atatattcag ggcacatttt tgtccggcgg
     1321 tacgacgatc atttgtgtag atttgaataa attgatgacg gacaaggaat acgctgtttt
     1381 acgtcacgaa gtcaaacatg cttaattacc acgaagtaca tccgatgggc accgggcatg
     1441 gcgttatcgc caaagaagcc gattttacgg atcaggattt tatccggttt cgtaatctga
     1501 ttcgcgcccg cagcggtatt tatttcaacg accgcaaacg tgccgatctc aaaagcggag
     1561 tattgaaagc gtttcactat tccggcgttt ccggtctgga taagtatttt gacttactgg
     1621 cgtcggatcc gacacagtcc gcgcagttta agacgcttgt ttcgttcctt accgtcggag
     1681 aaacgtattt tttcagacat tttgatgtga tcgaaaaagt cgttttaccg agccttatca
     1741 aagcacatca tcacgataaa accattcgca tatggagcgc cggttgttct accggggaag
     1801 agccgtatac cgttgcgatg gtattgcata atctgttacc ggatatccgg gattggaaga
     1861 tttctattgt tggtacggac atcaatgtga attcggtgga gtatgccaaa gagggagttt
     1921 acaggccgtg gtcgctgcgt tcgatcaccg atttttataa aaatttttat ttcgataaaa
     1981 aagacggatt gtactatatc aaagatttta tccgaaacat ggttcgcttc gactatctca
     2041 atctggtgga cgacggttat ccgcgggatg aaaacggcac caaagatttg gatctgattt
     2101 tttgtcgtaa tgtgaccatt tattttgaag ccgagaccac catcaaagtg gttaaccgtt
     2161 tttatgattg tttgaaggag aaatcgtttt tggccgtcgg tcatgcggag cccagttcgt
     2221 tagtctatga taagtatatt tccgaaatat atcccgatgc agtgatttac agaaaagaca
     2281 cggctttaaa aaaggagcag caatacaaaa caggtattcg tacgcgtcgc gatattttta
     2341 aggaattcaa agatcaaacc tcggtcgtaa aaaatcaggg tatcataaat aatctagata
     2401 cactgcaaca gagaattacc aaactcgata ttaccgatac tgttaaaaag gaaaaccctg
     2461 ttctcaaatc agagcccaaa ccaagagaac cactcttcaa agcggataat ctcacaatta
     2521 cgaccatgcg tgcaccgctg aaaccgcaac ccggcgcgaa accgaatgat cgtgatgcgg
     2581 tgatcgaaga aaaacggcgt gccgagctca acgaagcgga actgtttaca atggcactgg
     2641 aagaatttta taaaaaagat ttccgtgaag ccgaaaagca ctttaccgca ctgatcgaaa
     2701 aaaaccccgc gaacggcaga gcgctgtata tgatcgcgca catcaatgcc aatctcgata
     2761 atgtcagtaa agccaaagat ttttgccaca tggcgatcgc cgaagattcc ctgatgctgg
     2821 aagcgtatta cctgttggga ttgatttaca aagaagaaaa tctttttgac gaatcggtgc
     2881 gcatgcttaa gaagacgatc tatattgatt ctgattttgc gatcgggtat tacgagttgg
     2941 cggtcaatta ttttaaactc ggtgacggcg tgcaagcgcg aaaatattta aaacaaaccg
     3001 agcgtatttt aaaaaataaa tcggacgacg agcgcgtagg gattttggat gacttgaaag
     3061 tcaaagaact gcgcatgatg gttaatatgt gggataatta gtgataaacg tatggcggaa
     3121 ccaatgaagc aacaagatgt atttgacgaa gtccacaagc tgatggcaga acttttgtcg
     3181 tcacgcttga cgccggaggc tgaagctaaa attttggccg gccgtgccga aaaactcaaa
     3241 cgtgcgcata ccaacgaaga aacgggcgaa caaattagtg tgcttatttt ccagttatcg
     3301 tctgaatttt ttgctttaga aacgcagtat gtgtctgaag taagggtact cagcgaagtg
     3361 acgccggttc cctgtacgcc ggattttgtg ctcggaataa ctaatattcg cggtgccgta
     3421 ttttcggtga ttgatattcg cggttcattc aatttggaag accggcctgt cacggatcga
     3481 tctatgtttt tgatgattca gtggaaagga attgagctgt gcctattagc cgacgccgtg
     3541 attgaaaaaa tcaatttacc ccgttcggaa gtcaagacga tgaccggcgg caatagagaa
     3601 cacatgtcgt catttgtgag cggttttttt attcgcaatg ataataaaat tacgttgatc
     3661 agttgggata actatgtttc acattcgaat ataattgtca acgaagaagt ctaaactccg
     3721 gagaatccat atatgatttt tttgtttttt ttactcatgg cgatacctgc tgcagccata
     3781 ggcactttcg ttgtttatat ggtttacgga agaaaaaaat ttatccttag cgttttgata
     3841 cgcaccgtac cctggttggt actcatcgta tgtgcggcct ttttcatcgg tatatattcg
     3901 atgcactggt ggggctgggt tattggcctg ggcgcggctg taatttttgg cgttatttat
     3961 tatctggtgc tattccgcag tacttttaca gttacaatgg atatcgtgga tgtcgtaacg
     4021 cgtatcgcgg ataaagatta ttccaaacgt ttgacattca aggccgatgg ggaattgaat
     4081 accatgcgtg aaagtctcaa ctcggtcatc gataatttgg agaatacgga aaaaactttg
     4141 ggcgatgccg tatcgcgttt gtcatcggtt gtcagtgagc ttaactcgac gatggatcaa
     4201 caggttgtgg ggatgaatga gcaatctaca tcgctcaatc agacgacaac cacgacacaa
     4261 gaattggccg ccacttcgca tcagacaacg gaaaaagctc agtttgtcgt agagagtacc
     4321 gagcgcagta tggatattac gtccaaaggc aaagatgcgg tagacaatac cgtggatgaa
     4381 atgaatgaaa tcaaaaagcg tgttgaacgt atcgcggagc aaatacttga tctcagcgaa
     4441 aaaacgcaac agatcggcgt tatcaccacc accgtcaatg atattgccga acaaaccaat
     4501 atgcttgcgc tgaatgcggc tatcgaagcg agtaaggccg gcgaattcgg caaaggattc
     4561 ggtgtggtgg cttttgaagt ccggaagcta gctgaaaaaa gcaaagaggc ttctctgcgc
     4621 atacgtgatc tgatcaccca gatacaaaat gcaacgaatt cgacagttat ggctacggaa
     4681 gaaggctcaa agcgcgttga tatcggtgtt gaaaaaatac gagaagccgg aaaatatatg
     4741 gatgaatcca tccaaagttt ggaagaaagc gtaggttatg cccaacagat tttggttggc
     4801 agcaaacaac agacgatcgg tatcgaacag atcacgctag cgatggctaa tatcaacgaa
     4861 gtcgtgaagc aagtcgccaa aggaaccgcg caaacacaag gtgccgtgga ttccgtgtta
     4921 ggattgacca aagaaatgga tagtttagtc aaaaaataat cgttcatcat ccgggccaca
     4981 tattttacgg atttgcctat ggatttgtct aaagacgaac tcaaagaaat gatgtccatc
     5041 ttcaaggtgg agagcgaaga gcatctgaaa aaccttaata aaggtttgct caaacttgaa
     5101 gaaacgccca actcccgaga actgattgat gagctttttc gtacggcgca cagcatcaag
     5161 ggatcggcgc gtatgatggg ttttcagaag atcgaagggg tatcccacaa aatcgaagat
     5221 attttcggtt tagtacgccg cgcggaaatc aaagtatcac ccgaaaattt tgatgtcatt
     5281 tatgaagggg ttgatattat ttctcaaatt atcgaattga tatccaacga aggatcggac
     5341 gattcaatcg atatttcagt tatcgtaaaa aaattagaag ctatttataa cccgcaagaa
     5401 gcaccggctt ccgtagccga gtcgaaggaa gaatctaaac ccgatgtgga accggtgttg
     5461 gacgttgtgt ctaaagccga accggtaccc gtgacggaag cgaaaactcc tgagccggag
     5521 gagaccgaat ttaaaccgac gaccgatcta tccaagtcgg aggtgagtgt tgataaaacc
     5581 gacgcggcca aaggcaatgt ttccgaagat gcgtttattc gtgtgccgac gcggcgtttg
     5641 gacgatctga tgaatcaggt gagtgaactt gtcactacgc gcatcaaatc ccagcaacga
     5701 ttggcggaca ttcgcaaaat gctggatttt accgaggagt gggctcaaca aacggaacgc
     5761 attcgattat tgttagatcg tactttatca cgtatcgtca acgaagacgt cgagagacca
     5821 agtgacgtaa aaagcgaatt gatcacatcc tacgatttgc gaacggtagt tacttcgtat
     5881 ctctcatcca ttgagaaaat aggttcgatt gccgaatcca ttagcatttt gtatgacaag
     5941 cagagtgaag aaaatctgcg catgtccgtc atcacagggg atattcaaga taatttgcgc
     6001 attattcgcc ttttgccgat ttcgactatt ttcgatttgt atccgcgcat gattcgcgat
     6061 attgccaaat tacaaagcaa aaaagtgaaa tttgaagtct tcggtgggga tacgcgcgtt
     6121 gataaaaaag ttttagagga gttgaaagat cctctgattc acttattgcg taattctatt
     6181 gatcatggta tagaagcgcc ggccgatcgc gaagccactg ggaaagcaca agaaggcacg
     6241 atacaagttc gtgccggata cgtcggcaat atggtgcaga tcgaagtcca ggatgatggc
     6301 cgtggaattg ataccgacag gattttgcag atcgcgatca aaaaaggtta tatcaaggaa
     6361 catcaagcct ccgaagtcag taaaaatgat ttggtcgggc ttattttcca ttccggattt
     6421 tctacggcaa aaattatcac cgatctttcc gggcgcggtg tggggcttga catcgtcaaa
     6481 tccaatatcg aaaaaatcaa aggcacgatc gaaacggttt ctgaaaaagg aaaaggtaca
     6541 cgctttacga tcaaaattcc gctcacgctc gcgactacgc acgtgcttat cgtggatgtc
     6601 ggcggcgatc attattcgct tccgattgat tttatagatc gtacggtgcg attaacggaa
     6661 gaagaattgt ggacgggcgg gcaaaatcct tttgtcctgg tggataatac gccggtgcca
     6721 ttgtataaaa tggaagaggt gttgggcaat gccaaacaac gcctcaaaaa caaaggcttg
     6781 ctcaaatcgt ccgctgcgcc caaaatcggg cagcatctta caaaaataga gacgaataaa
     6841 tttcctgcgg ttatttttgt ttccggaggc cgccgggtag cgtttttagt ggataaactt
     6901 ttagatgaac aggaagtcgt tgttaaaagc ctcggtcatc aactcaaacg cgtaaaaaat
     6961 gttgcgggtt caaccgtgct tggtgacgga cggatttcga ttattttaga tcccaatgac
     7021 ttgatcaaat ccgttcaagg ttccggtacg aaattcgcct tccgggatcg ccgtcagcgc
     7081 gatttgatca aaaagcgcgt attggtagtg gacgattcga tcacgacacg tacattagaa
     7141 aaaaatattc tcgagtccgc cggttatcac gtgactatcg cgactaacgg tttggaaggt
     7201 tatcagaaac tgcatgaaat cggtaatttt gatattgtcg tgagcgatgt ggaaatgccg
     7261 tttatgacgg gatttgaatt tgcacacaaa gtgcgaaccg agtccaaatt tccggatatt
     7321 ccgtttgtat tgtgtacgtc actggagtcg gataaggata aacgccgcgg gatcgaggtc
     7381 ggcgctaatg cctatatcgt caaaggtgga tttgatcaaa ctaatctgct tgaaaccatt
     7441 gaaaagttgt tataggaatc aatgcccaag atactcatcg ctgaagattc accgaccgtc
     7501 acgatgatcc ttcagaaaat ttttgcgacg gatgatgatt gccaagtgat cggcgtggct
     7561 aaaaacggca gggaggcggt agaaaaagtt cagattctcc ggccggacat agtcacgatg
     7621 gatattcgta tgccggttat ggacggattt gaagcgacaa aacagatcat gcaaacatca
     7681 ccaacgccga ttttagtgat cagtgcgtct gtgggtaaag atgacctcaa tattgcgttt
     7741 aatgccattc gtgccggagc gttggatatt gtcgaaaagc ccaaaggcaa tctggcgatg
     7801 gattacgaat atattggtcg cgatttgatc aaaaaagtta aaatactttc cggtgtcaaa
     7861 gtttttcatc atatcggcgg cgctaaaaat aaatcgtcgg acgcgaaatc taatgataaa
     7921 accgacacat tatccgttcg tcagccgcgc gtgcctgccc cggctgccga agtgccgtca
     7981 gtcaaagaaa cactctggcg cgaaccttta acgcccaaga gcgtatatcc gaaactattg
     8041 agtcccgtaa aaacacccga ggtgatagct atcgcttcat cgacgggcgg accatcggcg
     8101 ttactcaaat tgctcaaaac cctcccggca ggatttccgg ttcctgtagt tattgtccag
     8161 catatttgtg aaggattcgg gcaaggattt gttgaatggc tcaataaaga gtgcggtatg
     8221 aatgtaaaaa cagcggatcg cggtgaagaa ttacaaccgg gaacgattta tgttgctccg
     8281 gacggctatc atctttttat tgattcaggc aaaatcgttc gcctcagcaa atccatgccg
     8341 gtgaatggtc ttcgccccaa tgcgacgctg atgatggaat cggttgctaa agtttttggt
     8401 gcggcttcga tcggtatagt tctgactggc atgggacgtg atggcgctga tggagccaaa
     8461 gccatcaaag aagccggagg ttttacaatg gcacaaaatg ccgaatcttg tgttgtattt
     8521 ggtatgccca aagaaacgat tgagttgggt gttgtagata aaattttacc ccctgaaaaa
     8581 atgacagccg agctgatggc ttttttcaaa ttatatcaaa cggctaacgc gtaagaggta
     8641 gttgtggcta aagaaaaaat attggttgta gatgacagcg cgacgcagct catcatgtat
     8701 aaaatggcgt tgagcaaagc gggatatctc gtcatctccg ccaaaaacgg cgtcgaaggc
     8761 atgcacatgg tggagacgga aaatcccgat ctgatcgtga gcgacatcat catgccggaa
     8821 ctcaacggat atcaattctg ccgattggtc aaagacgatc cttcccgtgc gcacattcct
     8881 attattttgc ttacaagcct cggtcagcag caagatcgtt tttggggcat tgaagccggt
     8941 gccaatgcgt ttgttaccaa ggcaactgat acgactgcgc tgctacaggc ggtagaagaa
     9001 ttgatcaaaa ctatcgcgaa gccttcaacc atagattatg ccgacaaaga agatgaaaaa
     9061 tattccattc agtcgccgga gaatccggaa gaaagtatca aatccaaact gaatcgcatc
     9121 ttagagaaac ttctttatga aacgacggta tctaaccgaa tgcgcgactt gtcgcggtat
     9181 gcttacaatc gtcaggaaat gattacgcgc tgttttgatc tctttcgaca gatcgtcgaa
     9241 tatcatgcat ctgcactcac gctttttgag aaagacacgc tgcatttgac gattgatatt
     9301 caaaaagaca tcgccgataa ttttttggat aaagccatcg catcggtatt gcagcaagat
     9361 tttaatccca acgaagtgcg tatgggactg atcaaaaata cccagaaaga tatttttggt
     9421 gaaacccacc ttaagccgga aggcgcacag gacgtccaat ccgtgctggt tgtgcctttg
     9481 cgggatgaaa cggaagtaat cggttccttg gctgttttta catcggatac atttgcgtac
     9541 aacaaagata tttaccggct catccagctg atcgcgcgtg aactgacatt gacgatcaaa
     9601 tatatttgga aattggaaga actcgaaaat atcaaacgta attttacatc gacgatcgtc
     9661 aatgatctgc gaagcccgat catcgccaat cagagctttg ccgaagcgct gtttaacgaa
     9721 tacgtcgatc cgatcaccga agatcagaga gaaatcctgt ccaacgtcat tgcgaataat
     9781 aaaaaattac tgacgttcat caacgacatc attgacattt cgaaaattga atccggtaag
     9841 ctggaaattt tcccggaaca caataatgtg catacgatca tcgaagatgc cataaaaaac
     9901 atgtccgtct tggcttccca aaaagaaatt ttacttatca atgaatttga aaatacggat
     9961 tacaaggcgt attttgacgg cgaaaaaata ttacaggtat tcaataatct gatttccaat
    10021 gctatcaaat ttacctccac cggcggagca gtaatgatcg gagcacaacc ggtgcctgat
    10081 ggcgtgcagt ttatggttaa agatacagga atcggcattc cggaagaaga cttgccgcat
    10141 atctttgaaa aatacaaacg aaccggtggt gtgttttcca gcgatgatca tgccggcggc
    10201 ggcggcatcg ggttgggact tgccatttgt aaatccatca tcgaagcgca taaaggccgc
    10261 atttgggctg aaagtgcggt cggtgaaggt accacatttt atttcaccgt attaacaccg
    10321 gaacaatcat aacgtatgct agacgatatt tttacaccgt ccgacgaaca agatatcacg
    10381 ttgaccaatg acgagcgtac ccgtattgag gggatattat ccaaactggt gacggaaagc
    10441 gcgcagcaag gcgtgagtat cgtagcattg aaaaatcaca aaatatctaa acctattttg
    10501 tttaaactgg ctaaagacgg taacagcaat gccagttatg ttttatcgga actcaatctg
    10561 cgcggcattc tggaggaagc caaaaatccc gatacgcgcg aagaagtggc cacggaaatg
    10621 ctggcgcgcc ttgtcgtttg tccggtaccg tccattcgcc ggcgggccat tcatctttta
    10681 ttgaaaatca atcctgaaaa attttctgaa gtcatcaatg aacgcaattt atcgccgaat
    10741 ctgaaaaatg aaattttttc ggatgtgatt gcggatgaag aagccggatt gcgcatcatg
    10801 cttcgtatgc tcaaaaatcc cggaacacct caggacatca aagataaagt gattgctaaa
    10861 atcggtcgta taagaaaaat gatcgaagaa aacagcgcgc agatcattga ctggaatcat
    10921 cgtatcgaag atcttgaaaa aagaaaacgt atcaaatttc acatggtcga aaaatattac
    10981 gccgaagttc agcaatctct ggacgaattc aaaaatcttc ccaattttgt gatggacctc
    11041 aagtttgatg tggatttggc ttttgaaaat cttaaacaca cgatcaagga atgaaatcgg
    11101 tcgtatcatc cgaggggcaa ccgtcactgt cttttgtcga agagtcatcc gctttttctg
    11161 tgtggccgca tgtgacatac cttgtgattt attttatg
//
LOCUS       DAOQGF010000105        10921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2802_length_10921_cov_6.902632, whole genome shotgun sequence.
ACCESSION   DAOQGF010000105 DAOQGF010000000
VERSION     DAOQGF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2802_length_10921_cov_6.902632"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /locus_tag="PK937_12535"
     CDS             complement(<1..2051)
                     /locus_tag="PK937_12535"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNH33445.1"
                     /translation="MISKEEVLEFHAQGRKGKIEVVPTKPCSTQRDLSIAYSPGVAVP
                     CLEIEKNPEDAYNYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKRFADI
                     DVFDIELDTKKVDEIIRVCQILEPTFGGINLEDIKAPECFEIEETLKKTMNIPVFHDD
                     QHGTAIISGAALLNATELVGKRMDEVRVVVNGAGASGIACANFYISLGVKPENLILCD
                     TKGVVYKGRKEGMNKYKEVLANDTPLRTLADALKGADVFAGLSVANVLTQDMVKTMAR
                     DPIIFAMANPDPEITYDDAKAARPDAIVATGRSDYPNQVNNVLGFPFIFRGALDVRAT
                     TINEEMKRAAAMALAKLAKEDVPDSVSKAYGDERLRFGREYVIPKPFDYRVLIWEAAA
                     VAQAAMESGVARIKVNIEEYKELLERRLGKSREVMRTMIHTARKHPKRVVFPEGAHEK
                     ILRAAHIILEEGIAKPILLGRRELIEKTAAELGVNISGAEIIHPSTSDNYLSYVEKYY
                     AMRQRKGATLTEARNAIRNYTTYGSMMVRLRAADALIAGVSQHYPDTIRPALQIVEME
                     EGISSVSGLYMLIIKNQIYFIADATVNIDPSVEELAEIAIVSARVARRFNTEPRVAMI
                     SFSNFGSARHPHAEKVRKAVEIVRQREPKLMVDGEMQADTAVDATILEKDYPFSTLKG
                     GA"
     gene            complement(2076..2606)
                     /locus_tag="PK937_12540"
     CDS             complement(2076..2606)
                     /locus_tag="PK937_12540"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH33446.1"
                     /translation="MRIVLLLAALNLSASLYAQDKKTPSSGFRAEFLMHLDQMEKKYT
                     DMANTIPADKYGYRPAEGVRSIGEVLMHCAGGNYFLLGFIGVKSDTPMNENFEKNADP
                     KKATETIKASFALVRNTVLNMNDADMDKTVKFFGGKDATIRNVLFTTMNHQHEHLGQL
                     IAYARANGITPPWSAN"
     gene            complement(2831..3349)
                     /locus_tag="PK937_12545"
     CDS             complement(2831..3349)
                     /locus_tag="PK937_12545"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HNH33447.1"
                     /translation="MMRHVVLVSSYLINFIIFISLTHAQEIQVPLHVQSQFYKKIFAY
                     SLTIPNSSNPTIAIIAPSHDAGENVKAAFAKSGMTEVNVLTSQEFLASSKRYDVAYLC
                     AGVRTSEVSAFCQGKKILTVTGSVEVYDAGKISIGLKLREGKCQILLNMDEVKKEGHR
                     FSAAFLNLTKSK"
     gene            complement(3445..4359)
                     /locus_tag="PK937_12550"
     CDS             complement(3445..4359)
                     /locus_tag="PK937_12550"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNH33448.1"
                     /translation="MIEYKRENLNAFPDEIFSLRDLKILHLHKSHLTELSDRFEFCPQ
                     LAEVSFYQNDLAKIPDSIFKLKNLQRLNFGDNRISVLSYMIGELSCLEMLDLGHNELN
                     EIPDEIGDLSTLQFLYLTDNNLTDFPQTLKRLISLRYLNISENSFSGLPTSIGELTSL
                     IELRASDNCIENMDIRWSGLTALRELHLQNNQLTKLPDGLGNLSLLRKLFLQNNELKK
                     LPSALAKLKNLAELNLRNNSLYELPKMIGDLVRLTDLDLRQNHLKDLPESLTRLPHLY
                     KLDLRWNEIKENNSIVEALREKGCVVYT"
     gene            complement(4363..4779)
                     /locus_tag="PK937_12555"
     CDS             complement(4363..4779)
                     /locus_tag="PK937_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33449.1"
                     /translation="MLKNSVYSVVIVWFSVFTCLAQEKDPLNLTALMASPNTVYEVRY
                     KDGDGIRKTLCYLRASFKPDHILLYIFDTASDSYKNLEVPKSAIYSVKASNESIGRHA
                     EEEKKQSPFTPKKLIIGGAIVCALIGGIVAVLLLSK"
     gene            4980..5588
                     /locus_tag="PK937_12560"
     CDS             4980..5588
                     /locus_tag="PK937_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNH33450.1"
                     /translation="MDYTLFSIFFVTTFLMALSPGPAVFLIMSQGMKFGVRGSMLGAL
                     GIEVMNALYFFISALGLSAALLAFPVIFQVLKWSGIGYLIFMGIKILITAKPRPMQTV
                     QVEADKKLFSQGIITQAANPKAVIYFGSMLPQFIAPDKSIALQFTVLAVVGCVMEFII
                     LSAYGWIAHKGAQFLPGPQAVLWQDRIAGICLLTVALVLITK"
     gene            5640..>6357
                     /locus_tag="PK937_12565"
     CDS             5640..>6357
                     /locus_tag="PK937_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH33451.1"
                     /translation="MAFAAIYFIWGSTYLGIKYAIETIPPFLMAGIRNFSPGLFLFIV
                     LKLRGEPSPERSHWRDASIIGALLLLGGNGLVTWAESYVPSGLAALLVATVPLWMSVI
                     NGFYGDKLMPTRREIVSILLGLCGLAVLIGPEEIIGGGRIHTLGAFALIAATLLWSFG
                     SLFSRRAHLPSSPMMTVSIEMMVGGGLLLILAVFTSDWHSVQWNAVSQKSILAVIYLC
                     VFGSLLGFTAYIWLLKHVAPN"
     assembly_gap    6358..6457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6458..6682
                     /locus_tag="PK937_12570"
     CDS             <6458..6682
                     /locus_tag="PK937_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH33452.1"
                     /translation="SLLGFTAYIWLLKHVAPNKVATYAYVNPIVAVLLGALMGGEELT
                     VRTLVSAGIIIFAVVLMTIKRTTTAQEKKS"
     gene            complement(6745..8115)
                     /locus_tag="PK937_12575"
     CDS             complement(6745..8115)
                     /locus_tag="PK937_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNH33453.1"
                     /translation="MLPFISINPSNGKKIQSYTETDKDMLNRVIRQSHERFILWSRTS
                     FTERANLLIKISDGLLRERENLAQLMALEMGKPITQGLAEADKCAWVCRYYAENAEAF
                     LKQSDIPTEAKKSYVCFSPLGVVLGIMPWNFPLWQVLRFAAPTWMAGNTIILKHAPNV
                     SGCAEAIARLVSEAGAPQGLYSVIHVQPDLIEDVISNPYIAAVTLTGSTAAGKSVAGL
                     AGRYLKKCVLELGGSDPYIILEDANLETAVDACVTSRLINSGQSCIAAKRFIVTAPVH
                     DDFVEQFVQTMQRKTYGDPQEGIYDIGPIARNDLREKLHSQVLTGKKEGAHVILGGEV
                     PERAGFFYPCTVITGVKKGMVLYHEETFGPVATIIRAENETEAIAMANDTMYGLGAAV
                     FTSDVERGEKIAKDHLLAGSCFVNAFVRSDPRLPFGGIKASGLGRELGLYGIREFVNV
                     KTVYVG"
     gene            8239..9876
                     /locus_tag="PK937_12580"
     CDS             8239..9876
                     /locus_tag="PK937_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNH33454.1"
                     /translation="MPTKKKTVKKVVRKTKPKAKKATKKTLAKTSARPAKKAKSVKPK
                     PKPAKKVTARKKVKSVSRKVVKKKSAGKKSSVASLKPKSPVFVNPNIFREYDIRGIVA
                     DDLQIEVVELLGKAYGTYMVDKGAKVVTVGRDVRTTSLALKNALVKGMMSTGLEVIDV
                     GEVPTPVLYYSIVFYKTDGGIMVTGSHNPREYNGFKMCLGLAPIFGTEIQRLRHIIDG
                     KAFATGNGTYHEKTIISQYIQMITEKIQLDKKFKIVIDAGNGTAGTIAPKIFRKLGCE
                     VIELYCEPDGTFPNHLPDPTKLEYIKDLQDLVVEHQADLGLGFDGDTDRVGAIDELGR
                     VVWADKLIALMAREINQKNPGQKILFDVKCSQGLIEDIQTHGGVPVMWKTGHSLLKAK
                     MKELHNPLAGEMSGHIFFADDFYGYDDAIYVAARLVQMISRAPHTLSELSDSFPKFYS
                     TPEIRVDTTEADKFRIVDEVRDYFRKKYEIIDVDGVRILYGDGWGLVRASNTQPVLVV
                     RFEAKTETRVHAIMNEVITKLREYPTVQINEADLIIA"
     gene            9905..10219
                     /locus_tag="PK937_12585"
     CDS             9905..10219
                     /locus_tag="PK937_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33455.1"
                     /translation="MKTVQGVYRNGKVEALEELKADNNTKLLIIVTDEKISDGVAPEL
                     QGVSVLEKIRRRQAADVSTEQQVSSREKLKQVSAKIDGKLPYENIEAASQSLRRYADD
                     HD"
     gene            10206..10685
                     /locus_tag="PK937_12590"
     CDS             10206..10685
                     /locus_tag="PK937_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33456.1"
                     /translation="MIMIDSDIFIMDNFYTNDPRYETNSLFLHRIQVQKNYTTIFNLL
                     EVCSLIAPHCTEKELLGFYENFDRTYNLEIIHPSTYDLSTEYFLDHFFGEIMYMTQNT
                     GNILDAMMFTLVRQRTIRLFVSWNAPRYKKMYAGRFGKLEFLQPDEYMKFYKVTGNR"
     gene            10689..>10921
                     /locus_tag="PK937_12595"
     CDS             10689..>10921
                     /locus_tag="PK937_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNH33457.1"
                     /translation="MSTKPIVECVPNFSEGRDTKVIQTITDAIEKTKGIRLLNVEPDK
                     DYNRVVVTFAGDPQPCVDAALAGMAAACETIDMR"
BASE COUNT         2906 a         2520 c         2354 g         3041 t
ORIGIN      
        1 gcgccgcctt tgagggtact gaacggatag tctttttcaa gtatggtcgc atccaccgcc
       61 gtgtccgcct gcatttcgcc gtccaccatg agtttgggtt cacgttggcg aacaatttca
      121 acggccttgc gtactttttc cgcatgcgga tgacgtgcac tgccaaaatt actaaaactg
      181 atcatggcga cacgcggctc ggtattaaac cgacgcgcca cgcgagcgct gacgatggcg
      241 atctcggcca gttcttccac actcggatca atattgaccg tcgcatcggc gataaaataa
      301 atctgatttt ttatgatcag catatacaaa ccgctgacgc ttgatatacc ttcttccatt
      361 tcgacgatct ggagcgcagg gcgaatcgta tccggataat gctgcgacac gcccgcaatg
      421 agcgcatcgg cggcacgcaa gcgcaccatc atcgaaccgt acgtcgtata gtttcgtatc
      481 gcgttgcgcg cttctgtgag ggttgcacct tttcgctgac gcatcgcgta atatttctct
      541 acatacgaca ggtaattatc cgacgtcgag ggatgtatga tctccgcacc gctgatattg
      601 acgccgagtt cagcagccgt tttttcgatc agctcgcggc gccctaaaag tatgggtttg
      661 gcgatacctt cttctaaaat aatatgcgcc gcccgtaaga ttttttcatg cgcaccttcc
      721 gggaatacca cgcgtttagg atgtttgcgt gccgtatgga tcatggtacg catcacttca
      781 cgggatttgc caagacgccg ctctaaaagt tccttatact cttcgatatt cactttgata
      841 cgcgccacac ccgactccat cgccgcttgt gcgacggccg ctgcttccca aatcagcaca
      901 cggtaatcaa acggtttggg aatcacatac tcccgaccaa atcgaagccg ctcatcgccg
      961 taggctttag atacggaatc aggaacatct tctttggcta acttagccag cgccatcgct
     1021 gccgcgcgct tcatttcttc attgatcgtg gttgcgcgta cgtccaatgc gccgcgaaaa
     1081 atgaacggga aacctaaaac gttattcact tgattaggat aatcggaacg accggttgcg
     1141 acgatcgcat caggccttgc ggccttagcg tcatcatagg tgatctccgg atccgggttg
     1201 gccattgcaa aaatgatggg atcacgggcc atcgttttca ccatgtcttg ggtcaataca
     1261 ttcgccacgg acaatccggc aaatacatcg gcgcctttga gtgcgtccgc taatgtgcga
     1321 agcggcgtat cgttggccaa aacttctttg tatttattca taccctcttt gcgtccttta
     1381 tacacgacgc ctttggtatc gcaaagaatc agattttccg gtttcacgcc caagctaata
     1441 taaaaattgg cgcacgcgat tcctgaagct cccgcaccgt tgacgacgac gcgcacttca
     1501 tccatacgtt tgcctacaag ttccgtcgca ttgagcaatg ccgctccgga gatgatcgct
     1561 gtcccgtgtt gatcgtcatg aaacacgggg atgttcatcg tttttttgag tgtttcttcg
     1621 atttcaaaac attccggcgc tttgatatct tcaagattga taccgccaaa tgtcggctcg
     1681 agaatctgac agacacggat gatttcgtcc acttttttag tatctaattc gatatcgaaa
     1741 acgtcaatat cggcaaaccg cttgaatagc acacctttgc cttccatcac cggtttaccg
     1801 gctaaagcgc cgatatcacc gagccccaac accgctgttc cattggaaat gacggcgact
     1861 aaatttcctt tagccgtgta gttataagcg tcttcagggt ttttttcgat ctctaaacat
     1921 ggcacagcca cgccgggact gtatgcgatg gacagatcac gctgggtcga gcagggtttg
     1981 gtcgggacaa cttcgatttt ccctttgcgc ccctgggcat gaaattcgag aacttcttct
     2041 ttggaaatca tggtactcac tttggtttac acgaatcagt tggcgctcca cggcggcgta
     2101 ataccattcg cacgcgcgta cgctataagc tggccaaggt gttcatgttg atgattcatt
     2161 gtcgtaaaaa gtacattgcg aatggtggcg tctttgcctc cgaaaaattt taccgtctta
     2221 tccatgtcgg catcgttcat gtttaacacc gtgttgcgca ccagtgcaaa cgaagcttta
     2281 atcgtttccg tggctttttt ggggtcggca tttttttcaa aattctcgtt catcggtgta
     2341 tcggatttga cgccgataaa tccgagtaaa aaataatttc ccccggcaca atgcatcaat
     2401 acttcaccga tggatcgtac gccttcagcg ggacggtacc cgtatttatc cgcaggaatg
     2461 gtgttcgcca tatccgtgta tttcttttcc atttgatcca aatgcatgag aaattcggct
     2521 cgaaaaccgg aggagggcgt ttttttatcc tgagcgtaaa gcgaagcgga taaattcaat
     2581 gcagctaata atagtacgat acgcatggtc agactccttt tgttaatagt aagaccgcgt
     2641 aaagtacaag gatttagaaa taaaacaaac atttcgaaaa gattaaatta cacttttctg
     2701 aatgagccaa tatcccctac aatcacagat gaatgaatga caaaaatcat gttctgtgga
     2761 attgattcta tgactatgtt agagaatgta gaatgtggtt ctggctcaga ataacgtatg
     2821 tggatgactt ctatttggat ttagtcaaat taagaaacgc agcggaaaaa cgatggcctt
     2881 cttttttaac ttcatccata ttgagcagta tctggcactt gccctcacgg agtttcaaac
     2941 caatactgat tttaccggcg tcatatacct ccaccgatcc ggtcaccgtc aaaatctttt
     3001 taccctgaca aaaagccgat acctcgctcg tacgcactcc ggcacataga tacgcgacgt
     3061 catatctttt tgaagaggct aaaaattctt gcgacgtcaa aacattaacc tccgtcatcc
     3121 ccgatttggc aaatgctgct tttacgtttt cgcccgcatc atgtgatggc gcgatgatcg
     3181 ctatcgtagg gttactacta ttaggtatcg tcagagagta tgcgaatatt tttttataaa
     3241 attgggattg aacatgcaac ggcacttgaa tctcctgcgc atgggtcaat gaaataaaaa
     3301 taatgaaatt gataagataa gaggaaacta agacgacatg ccgcatcata ataagcgcct
     3361 ttcctctgga tagatgaatt cgaagaagtg atgccgtaaa tatacggcat tggaatggct
     3421 ttgtaaatac gtaaactcat gtattcaggt ataaaccaca caaccttttt cgcgtaacgc
     3481 ttcgactata gagttgtttt ctttgatttc attccagcgc aaatccagtt tatagagatg
     3541 cggtagtcgg gttaaagact ccggtaagtc ttttaaatgg ttttgtcgaa ggtcaagatc
     3601 tgtcaaacga actaagtcgc caatcatttt cggcaattcg tatagactgt tattccgcaa
     3661 attaagttct gccaaattct tcagcttcgc cagcgccgac ggtaattttt ttaattcatt
     3721 attttgtaaa aaaagttttc tcaataacga taagttgccc aaaccatccg gtaatttggt
     3781 caattgattg ttttgcaaat gcagttcgcg aagcgccgtc aatccgctcc accgaatatc
     3841 catgttctca atacaattat cgctcgctcg caattcgatt agcgaagtaa gttccccaat
     3901 tgaagtcggt aaaccactaa atgaattctc tgatatattc agataccgga ggctaattag
     3961 tctttttaat gtttgcggga aatcggtcaa attgttatct gtgagataga gaaattgcaa
     4021 agtgctcaaa tcaccgattt catcgggtat ttcattcagt tcattgtgtc cgagatcaag
     4081 catttcaagg caggaaagtt caccgatcat ataggagaga acgctaatac gattatcacc
     4141 aaaattgagt ctttgtaagt ttttaagctt aaaaatactg tcgggaattt tagctaaatc
     4201 gttttgataa aagctcacct cggcgagctg agggcagaat tcaaaacgat cagatagctc
     4261 agtaagatgg cttttatgaa gatgtaaaat tttgagatcg cggagtgaaa aaatttcatc
     4321 cggaaacgcg ttcaggtttt cgcgcttata ttcgatcatt gtttatttgg acaaaagtaa
     4381 tacagcgacg atccccccga tcaaggcaca aacgatcgca ccgccgatga tcagtttttt
     4441 gggtgtaaat ggtgattgct ttttttcttc ttcagcgtga cgtcctatac tttcattaga
     4501 tgctttgacg ctgtagatgg cagatttagg aacttctaga tttttataac tgtccgaagc
     4561 cgtatcgaag atgtatagta aaatatgatc cggcttgaat gatgctcgca aataacatag
     4621 agttttgcgt ataccatcgc cgtctttata tcgtacttca tatactgtat tgggtgatgc
     4681 catcaacgcc gtcagattta ggggatcttt ttcctgcgcc aaacaggtaa ataccgaaaa
     4741 ccacacaatc accacgctgt aaacagaatt cttcagcata actcgctcca atataagtga
     4801 tacaatgaat gtgtggattc gttaagacaa tagcaagttt cgggttggta taaccgatga
     4861 aatgcactat gatgaatcca gtgatcaatg gactatgatc ttttgacgta gaatggtact
     4921 tgtgccggtt attcattcgg cttatctttg attttaaaaa aaaatttaaa aagccctgca
     4981 tggattacac tctattttcg attttttttg tcactacttt tttgatggcg ttatcgccgg
     5041 gtccggcggt ttttctcatt atgagtcagg gcatgaagtt tggcgtgcgc ggaagcatgc
     5101 tcggtgcgct gggtattgag gtaatgaatg cgttgtattt ttttatttcg gcacttggtc
     5161 taagcgccgc tttgcttgct tttccagtta tatttcaggt tctaaaatgg agcggcatcg
     5221 gatatctcat atttatgggc atcaaaatat tgatcacggc caaacctcgc cctatgcaaa
     5281 cggtccaagt tgaagctgat aaaaaattat tcagccaagg catcatcaca caagcggcca
     5341 atcccaaggc ggtgatttat tttggatcca tgttaccgca atttatcgct ccggacaaat
     5401 ccatcgcttt acaatttacc gttttagccg ttgtaggttg tgtcatggag tttatcatcc
     5461 tgtcggcgta tggatggatt gctcataaag gtgcacaatt tttacccgga ccccaagccg
     5521 tactctggca agatcgcatt gcgggaattt gtttactgac cgtagcgttg gttcttatta
     5581 ccaaataaac cttatcggag atcatcatcg gaaattcatc acatcgtctg aaaacgcttt
     5641 tggcctttgc ggctatttat ttcatttggg gttcgacgta tctcggcatc aagtatgcga
     5701 tcgaaactat cccgccattt ttgatggccg gtattcgaaa tttttcaccg ggtttatttt
     5761 tgttcatcgt attaaaactt cgcggtgaac cctcaccgga acgttcccac tggcgtgacg
     5821 cctcgatcat cggcgctttg cttctgcttg gcggaaatgg tcttgtaacc tgggccgaat
     5881 cgtatgtacc gtccggctta gccgctttgc ttgtagcaac cgtgccacta tggatgtccg
     5941 tgatcaatgg attttacggt gataagctaa tgcctacgcg ccgtgagatc gtaagtattc
     6001 ttcttggttt atgcggttta gctgtgttga tcggacctga ggaaattatt ggcggcggta
     6061 gaatccacac cttaggcgca ttcgctttga ttgcagccac gctcctttgg tcatttggtt
     6121 cgcttttttc acgacgtgct catttgccaa gctcaccgat gatgactgtg tctatagaga
     6181 tgatggtcgg cggcgggtta ttgctcattc ttgccgtatt taccagcgac tggcattccg
     6241 tacaatggaa cgccgtatcg caaaagtcca ttcttgccgt tatttatttg tgtgtttttg
     6301 gatcgctgct tggatttacg gcgtatattt ggcttctcaa acacgttgct cccaatannn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg ctgcttggat ttacggcgta
     6481 tatttggctt ctcaaacacg ttgctcccaa taaagtagca acgtatgcgt atgtcaaccc
     6541 catcgtagcc gttttactgg gtgccttgat gggcggcgag gaattgaccg tgcgaactct
     6601 agtatcggcg ggcattatta tttttgccgt agttttgatg acgatcaaac gaacaactac
     6661 agcacaagaa aaaaagagtt agttttacca aaaaaacatt tctttccaag cgcgacagta
     6721 tacggatttt gttcaacaac atattcaccc tacatatacc gttttaacgt ttacaaattc
     6781 gcgtatgcca tacaaaccca attctcgtcc caatccggat gctttgatgc cgccaaatgg
     6841 taatctcgga tcagagcgca caaacgcatt aacgaaacac gaaccggcta aaagatgatc
     6901 cttggctatt ttttcaccgc gctcgacgtc ggaagtaaat acggctgcgc caagcccgta
     6961 catcgtatca ttggccattg cgatagcttc tgtttcattc tccgcgcgga ttatagtagc
     7021 cacaggtcca aaagtctctt catgatataa aaccattcct ttttttacgc cggtgattac
     7081 cgtacaggga taaaagaagc ccgctctttc cggtacctca ccgcccagta ttacatgggc
     7141 accttctttt tttcctgtta gaacttgcga atgaagtttt tcacgtaaat cgtttcgcgc
     7201 gatcggcccg atatcgtaaa taccctcctg cggatcgcca tacgtttttc tttgcatcgt
     7261 ctgaacaaat tgctccacaa aatcgtcatg gacaggcgct gtgacgataa atcgtttggc
     7321 cgcgatgcaa ctttgaccgc tattgatgag gcgtgaagtg acacaagcgt ccacggccgt
     7381 ttccaaattc gcatcttcta aaatgatata cggatcgctt cctccaagct caagtacgca
     7441 tttttttaaa tatcgcccgg ccaaacccgc gaccgacttt cccgcggctg tactgccggt
     7501 aagtgtcacc gctgcgatat aaggattaga aataacatct tcgatcagat caggctgaac
     7561 atgtatcacg gagtacaatc cttgcggtgc gccggcttcg gataccagtc gggcaatggc
     7621 ctccgcacat cccgaaacat tcggggcatg tttgagtatg atcgtgttac ccgccatcca
     7681 tgtcggcgct gcaaatcgca acacttgcca caacggaaaa ttccacggca taatgcccag
     7741 taccacgcct aatggagaaa aacaaacgta actttttttc gcctccgtgg gaatatcgct
     7801 ttgtttgaga aaagcttctg cgttttcggc ataatacctg caaacccatg cacatttgtc
     7861 ggcttcggct aacccctgcg ttatcggctt acccatttcc aaggccatca gctgtgccaa
     7921 attttccctc tcacgaagca gcccgtctga aattttaatt aacagatttg cacgttcggt
     7981 aaaacttgtt cgggaccaca aaataaatcg ctcatgagac tggcggatca ccctgttgag
     8041 catgtcctta tctgtttctg tataactttg tattttttta ccgttggatg gattgatact
     8101 gataaatggt aacatgtttt ttgtgtgttt tagttcttga taaagccgtc attcagcttt
     8161 aaattttaag gcttttttct cactaaacaa acaatttact gccgttataa atactagcct
     8221 acgcaccgga gatccatcat gccgaccaaa aagaaaaccg taaaaaaagt agttcgcaag
     8281 accaaaccca aagccaaaaa agcgaccaaa aaaactttag ctaaaacatc tgctcgtcct
     8341 gcaaaaaaag cgaaatccgt aaaaccaaag cctaaaccgg ccaaaaaagt tacggcgcgt
     8401 aagaaggtta aatcggtttc ccgcaaagtt gtaaaaaaga agagcgccgg aaaaaaatca
     8461 tccgtcgctt cgcttaaacc caaatcacct gtttttgtaa atcctaatat attccgtgaa
     8521 tacgacatac gcggcattgt cgccgatgac ttacagatcg aagtcgtcga attactcggt
     8581 aaagcatacg gcacgtatat ggtggataaa ggcgccaaag ttgtaaccgt cggacgggat
     8641 gtccgaacta cgtcactcgc gctcaaaaat gcattggtca aaggtatgat gtccaccgga
     8701 cttgaagtga ttgatgtcgg cgaagtgccc acacccgtac tttattactc tattgttttt
     8761 tataaaaccg acggcggcat catggtcacc ggcagccaca atccgcgtga atacaacggg
     8821 ttcaaaatgt gcctcggctt ggctcctatt tttggcacag agattcagcg cctccgccac
     8881 atcattgacg gcaaagcttt tgctaccggc aacggcacct atcatgaaaa aacgatcatt
     8941 tcgcaataca ttcagatgat taccgaaaaa attcaactgg ataagaaatt taaaatcgtg
     9001 attgatgccg gcaatggtac ggctggcacg atcgcaccca aaattttccg caaactgggt
     9061 tgtgaagtga tcgaattgta ttgcgaaccg gacggaacat tccccaacca ccttcctgat
     9121 ccgaccaaac tggagtatat caaagatctt caggatctcg tggtcgaaca tcaagccgat
     9181 cttggccttg gatttgacgg cgatacggac cgcgtaggcg ctattgacga actcggccgc
     9241 gtcgtttggg cggacaagct gatcgctttg atggcccgcg aaatcaatca gaaaaacccc
     9301 ggtcagaaaa ttctttttga tgtcaaatgc tcgcaaggtc tgatcgaaga tattcaaacc
     9361 catggcggtg ttccggtaat gtggaaaacc ggccattcgc tgcttaaagc taagatgaaa
     9421 gaactccata atccgctcgc cggtgaaatg agcgggcata ttttctttgc tgatgacttt
     9481 tatgggtatg atgacgcgat ttatgtggcc gcacgcctgg ttcagatgat aagccgtgca
     9541 ccgcataccc tatctgagct ttcagatagt tttcctaaat tttattcaac gcctgaaatt
     9601 cgcgtggaca ccaccgaggc ggataaattt cgcattgtgg atgaagtccg agattatttc
     9661 cgtaagaaat atgaaatcat tgacgtagat ggtgtacgaa ttttatatgg cgatggttgg
     9721 ggtcttgtgc gtgcatccaa tacacaaccg gttcttgtgg tacgattcga agccaaaacc
     9781 gaaacccgcg ttcatgccat tatgaatgag gtgataacca aattacgcga atatccgacg
     9841 gtacaaatca atgaagccga tctgatcatc gcatgatacg acgtatgtaa ccggagtaca
     9901 ccgagtgaaa accgtacaag gcgtttatag aaacggaaaa gtggaggctt tggaggaact
     9961 caaagccgac aacaatacaa aactgctcat catcgtaacg gatgaaaaaa tctccgatgg
    10021 cgtcgcaccg gaattgcaag gtgttagcgt attggaaaaa ataagacgcc gtcaagcagc
    10081 ggatgtctct accgaacaac aagttagttc acgtgagaaa ctaaaacagg tctcggccaa
    10141 aatagacgga aaactgccct acgaaaatat cgaagcggca tcccaaagcc tgaggagata
    10201 cgccgatgat catgattgac agcgatatct ttatcatgga taatttttat accaatgatc
    10261 cacgatacga gaccaatagt ttatttctcc accgcattca agtccaaaaa aactatacta
    10321 cgattttcaa tctgctcgaa gtctgcagct tgatcgcacc gcactgtacc gaaaaagaac
    10381 ttttaggttt ttatgaaaat tttgatcgga cgtataactt ggaaattatt cacccaagca
    10441 cttacgatct ttcaaccgaa tattttttag accatttttt cggtgaaatc atgtacatga
    10501 cccaaaatac gggaaacata ttggatgcca tgatgtttac attggtgcgt cagcgtacga
    10561 tacgtctttt tgtttcatgg aacgccccgc gttataaaaa aatgtatgcc ggtcgtttcg
    10621 gaaaattgga gtttttgcaa ccggacgaat atatgaagtt ctacaaagtc acaggaaacc
    10681 gatagcttat gtcaaccaaa ccgatagtcg aatgcgtgcc taatttcagc gaaggccgcg
    10741 ataccaaagt aatacaaacc attacggacg ccatcgagaa aacaaaaggc atccgcttgc
    10801 ttaatgtcga acctgataaa gattacaatc gcgtggtcgt aacctttgca ggtgacccgc
    10861 aaccctgtgt ggatgcggct ctagccggta tggccgccgc ttgtgaaacg atcgacatgc
    10921 g
//
LOCUS       DAOQGF010000106        10770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2848_length_10770_cov_6.254223, whole genome shotgun sequence.
ACCESSION   DAOQGF010000106 DAOQGF010000000
VERSION     DAOQGF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_10770_cov_6.254223"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="PK937_12600"
     CDS             <1..1563
                     /locus_tag="PK937_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33458.1"
                     /translation="RKVLSDQNKKNKSRIDSIFHNTDTSVTSYADAYQAYFESNPNGY
                     FSGDFKRNMTAGRFSVGPQSFQFGVSFVKTKDQVSSLLMNPITAVGYNGLTPQENLVA
                     GADFRASMFNKRLNFDASVASAISNTDITSGSISHADLDSADIDLTKSQYDLVKKFIT
                     LNANLDPLITEFDKNLLAYTVGSSISAYNNNLSVRYRFNGAFFQSYGASVARDQESFE
                     IVDRMRFWQNRIFLTINYATMKNNLAKTVANTPKTNTLGFNASFFLPNKLPSVTLGYQ
                     TMNRDNGFEYTGSATSANSKYKDQIRANARPEDNTTNTLSFNTTYGFTFSELRNSASF
                     SLSNSTRKDNSKDIGTAFLFETEASVIAEDTIVDYYKAAAGSKSNTIGLSLSTDWKFP
                     LRTSIGFSNTSGETKEIKKSDDNKRDSLTITKKTGAFSLNANGDYLVFQNDDVRINTT
                     AGYNFASVTIPNVPDVTLQTISLGSRINFFEKNNVTLSYSITSGLKIPTASGGSKSVT
                     NSLFLARYEYIF"
     gene            1647..4043
                     /locus_tag="PK937_12605"
     CDS             1647..4043
                     /locus_tag="PK937_12605"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNH33459.1"
                     /translation="MAKGDFFKNLRANTGALICLIISLLVFIPGLVLKVLPDLSDNLI
                     AFYLLNIGLTFASLLASVIGIYLAGFQNKTLSGIFITLLAVWLGSFLVNVKAIESVEL
                     KTLDQRFSFRYQYIDQEKRLTPEESPIIIVDVSDQAGEEVRDRYPWPRTYYAHFIRNM
                     KRAGAKVVGIDIVFDAPDYNGPEKDDDLAAAIKECGNVVLAGKVIKGDVASGGGSIGT
                     TAKPLPKFLDEGIGKWGFVGVTNDIDGFWRQYLVEEKTGDDEHEQVFHSFGVEVLRKY
                     YDIPDSSKFINNEQFFQFGDKVIRKYKPYSFLINFAGPKATFPYKSFELTVDDAAFDL
                     KEGRDLDSFDDEGDPDLGIPPGLLKSGFFKDKIVLLGATMEELHDVFSVPISEVRTSD
                     GKKLETLMPGVEIHANAIYTIIKDNYVTIQADWIRVLIVTLSAFVVFLLVSRLNKPLL
                     ALPILLLIIASVIALAFYLFAKHNIHMQLVTPVLAIMFTYVGNVIFQYLGERKEKAQI
                     RNAFGRYLPEKVVAQLVENPGLLKLGGEERVLSILFSDVAGFTTISEKLTPVELVHLL
                     NEYLTAMTNIISKYDGIIDKYEGDAIMAEFGAPLQDDEHAAKACYAALEMQEKLVEMR
                     VKWKREGRPELKARAGINSGNVVLGNMGSESVFDYTVMGDNVNLASRLEGANKEYGTY
                     IMISEMTLQFVKEQVITRELDILRVKGKAKGVRVFEVIARTSTGITANMKKTIENYNK
                     GLELYRQQRWDEAMVLFKTALTTNPDDGPSQTYIERCEEFKLNPPPENWDGIFEMKTK
                     "
     gene            4142..4963
                     /locus_tag="PK937_12610"
     CDS             4142..4963
                     /locus_tag="PK937_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33460.1"
                     /translation="MIERSNFSKDKSIDLDQPESIIDWFWVFWGCVIGIVIIGAFTFI
                     TSTKYNPDVVKAVPMLIGGLAFAVTGMILGHYSPGHTVKEAAVAGLVIPIIGFVLSGM
                     GYGPTFIAELSIFEKIGVALAGMLICQLGGWVGEELEGYDHPTKWLQWHWIIVAVVIG
                     FMLNSFVIFFVGLISLTPVPVFMALSVFAAGIVAGYKSPGHTEKECSIAGALTILLDY
                     LFITFALGIEAKDFGIESGTMAIIVLVALAVAGGLGLLGGWIGERMQIQEKELNN"
     gene            complement(5046..5798)
                     /gene="rlmB"
                     /locus_tag="PK937_12615"
     CDS             complement(5046..5798)
                     /gene="rlmB"
                     /locus_tag="PK937_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNH33461.1"
                     /translation="MKTNIIFGRQPVFEALKSGIPVKEVILQIGAHGDAIDEILNVCK
                     RKQIRVRTMDARYFSDMIGGKNNHQGAMVLLDQKEFQYVEMDVIFERAAVAGEKPLVA
                     ILDEITDPHNLGAIIRSAECAGFHGVVIPKHRSAEVNATVVKTSAGATAYIPVVQVTN
                     LTQALEELQERGVWIYGTSLDGDRDYDAIDVRDAVGIVIGSEGKGMRKMVAEHCDFLI
                     RIPMSGQIQSLNASVAAGIVFFDVMRQRRKGR"
     gene            complement(5788..7410)
                     /locus_tag="PK937_12620"
     CDS             complement(5788..7410)
                     /locus_tag="PK937_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNH33462.1"
                     /translation="MFIVSRYVIREHIGPFFFALALITSMLLLNFVLQAMRYIIGKGI
                     SMTVILEFIAYNLAGIIVLVVPMSILVATIMAFGRLASDNEVTALKAGGINFFKLIFP
                     VILVACIVTYGVFWFNDEVLPLANHNARVLKKNIQAKRPTLSIEPGVFLEGIENFSMI
                     VENKDELGSTIYGVTIFDKSDRNAQRTITAKRGSIAIEESNDNMILELEEGEIHEYHQ
                     GKNDQYQRIIFQKYKVIVAVENLSLKTSDESFRNDREKNIDQLMEEVARHRKERERQM
                     GLVLKNIEKKPESVTQLDPRARKLVEKYCVEFSWLDAINDYFMDWNLRLALDTAYVPY
                     TDYVLSDSLKQTIQTYLKDTLKHTVPVAESQPSALRSLDPSLGRRPNMSKSAIPVDSV
                     EMPLTAISNQVSGNISTVSSYQRLMDQSMVEVNKKYSIPMACLIFVLLGAPIGVKAKR
                     GNLGLAAGISLFFFIAYYFCLILGEDLADRGLMNPFFAMWFMNFFLGMLGCVLIYQTV
                     TEKTMGWLTIRSFFHSLKLTMKSSFKRKINEN"
     gene            complement(7428..8012)
                     /locus_tag="PK937_12625"
     CDS             complement(7428..8012)
                     /locus_tag="PK937_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNH33463.1"
                     /translation="MELQVEIKWIIEDEQSNGYILQLVETGPEKGRMLPVVIGAPEAH
                     SIALELQGIKPFRPFTHDLAVQILDALDAEVDKVVITELNKNTFFASIHIRSNSVGEQ
                     VIDSRPSDAIAIAIRVGSPIFVEEKVMEAAGLDVDVQSGAYVKQTKKVPASEASELSM
                     TPLEKLEKDLQKAVESENYELAAKLRDQINLLKS"
     gene            complement(8064..8909)
                     /locus_tag="PK937_12630"
     CDS             complement(8064..8909)
                     /locus_tag="PK937_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNH33464.1"
                     /translation="MQKIKTCAVEYLNTRPIVYGLQQAAAARFELQYAIPSVCADKLK
                     NKEVDLALIPSIEYSRIRGHRNLRIVPDIGVISHQFVKSVELFFNRNLENINTVAVDI
                     SSRTSVGLLDIILREKFDIHPEFIPMPPQVEVMLSKADAALIIGDNALELYDTWDNKI
                     DLGEEWGDMTDGLPFVYSFWAAAEDALNTDDILTLTASRDAGLDHITEISRVYSQERA
                     RGFEPDFYANYLTENIQFKLGKAELEGLKEYYSYCFYYGIIDEIPDLLFVGQENESDF
                     TVKNN"
     gene            complement(8915..9217)
                     /locus_tag="PK937_12635"
     CDS             complement(8915..9217)
                     /locus_tag="PK937_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33465.1"
                     /translation="MSELIEGWAVVKTVSYEHQAELISDQLQSAGIENTVFSQKDSVN
                     TVWVGDLSEIKILVPHDKLQDALDVCKDIELPDMELSEDADVGESEEDEDRDENEE"
     gene            complement(9231..10085)
                     /locus_tag="PK937_12640"
     CDS             complement(9231..10085)
                     /locus_tag="PK937_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33466.1"
                     /translation="MTKSAPFLFYIILLFSTCPIAFGQEQDDNDDDYTVQLENWKQKR
                     KETDSLNQSRRVAREQARGYYKEGNRLSKAKRWNEAISIYHKAITADSSYQKAYVNLG
                     LAYRRTGEISRAIAYYDLAIHLPNANTNITAKAVQYKVWLLSDVKNYSLAIETCNDFL
                     KNNAVHPEISYIKAKLLKDQLNRTDEAVAILEQIVSHHPVHAASLYELSNCYNEAKEP
                     ERALRLINQAGNIEEEKWQYGIFFERAEALRLLNRIEEALADYRKAANDKRWKESAEY
                     WIGVLQPK"
     gene            complement(10119..10200)
                     /locus_tag="PK937_12645"
     tRNA            complement(10119..10200)
                     /locus_tag="PK937_12645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10164..10166),aa:Leu,seq:gag)
     gene            complement(10292..10603)
                     /gene="secG"
                     /locus_tag="PK937_12650"
     CDS             complement(10292..10603)
                     /gene="secG"
                     /locus_tag="PK937_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNH33467.1"
                     /translation="MFAFLVVLHIIVSILLIGAILMQSSKGGGLAGLGGGASTILSGR
                     QAADTLVKATVVLAILFAVLSVSLNIKGLRDGGAEQGIIEKELEQGGGQSTPALPTPQ
                     N"
     gene            complement(10640..>10770)
                     /locus_tag="PK937_12655"
     CDS             complement(10640..>10770)
                     /locus_tag="PK937_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNH33468.1"
                     /translation="GSVKPDNAAALLGCVDIDGALVGGASLEAESFAAIIHAAEKI"
BASE COUNT         3015 a         2427 c         2203 g         3125 t
ORIGIN      
        1 cgtaaagtat tgagtgatca aaataaaaag aacaaaagca gaatagattc tattttccac
       61 aatacggaca cttccgttac ctcttacgcc gatgcttatc aggcttattt tgaaagcaac
      121 cccaatggtt atttttcagg cgatttcaaa cgtaatatga ccgccggacg cttctctgta
      181 ggcccccagt cattccaatt cggagtaagt tttgtaaaaa ccaaagatca agttagctca
      241 ctcctcatga atcctataac ggctgtgggc tataacggct tgaccccgca agaaaatttg
      301 gttgccggtg ctgattttcg agcaagcatg ttcaataagc gtttgaactt tgatgcatcg
      361 gtcgccagcg ctatcagtaa caccgatatt acatccggtt caatttctca tgccgatcta
      421 gactcagcag atattgacct gactaaaagt caatacgatt tagtaaagaa gtttattacg
      481 ctaaacgcta atctcgatcc actaataact gaattcgata aaaacttact cgcatatact
      541 gttggtagtt caatttctgc atataataac aatctgagtg tgcgttatcg ctttaacggt
      601 gcgtttttcc aatcatacgg ggcatcagta gctagagatc aagagtcgtt tgaaatcgtc
      661 gaccgcatgc ggttttggca aaaccgcata ttccttacaa tcaactatgc gacgatgaag
      721 aataaccttg ccaaaaccgt cgccaataca ccaaaaacta acacgctcgg atttaatgcg
      781 tctttcttct tgcccaataa attaccatcg gtgacattgg gttatcaaac catgaaccgt
      841 gataacggct tcgagtatac cggttctgct acttcggcta actccaaata taaggaccaa
      901 atccgtgcaa atgcgcgccc ggaagacaat actactaata cactcagttt caacactacc
      961 tatggtttta cgttcagcga actgcgtaat tcggcgtcgt ttagcttatc caacagcaca
     1021 cgtaaagaca acagcaaaga cataggtacg gcatttctct ttgagaccga agcttcagtc
     1081 atcgctgaag atacgattgt ggattattac aaagcggctg ccggatcaaa gagcaatacc
     1141 atcggcttga gtctttcgac ggactggaag ttcccgttac gtacttcgat cggatttagc
     1201 aatacgagcg gtgaaactaa agaaattaaa aaatcagacg ataataaacg ggattccttg
     1261 acgattacaa aaaaaaccgg cgcattcagt cttaatgcca atggcgacta tctcgtattt
     1321 caaaacgatg atgtgcgtat taatacgaca gccggataca attttgcatc ggtaacgata
     1381 cccaatgttc ccgatgtaac tctacaaacg atttctttgg gttcacgaat caatttcttt
     1441 gaaaaaaata atgtgacgct gagttacagc attacctccg gactcaaaat tcctacggct
     1501 tccggtggtt ccaaatcggt aaccaacagt ctattcctgg cacgatacga atacattttc
     1561 taattcgatc ttatccgcga gagcctgatt tgattacaaa tcaggctctc tttttatcca
     1621 ctctactatc tttatgacga ggcattatgg caaaaggtga ttttttcaaa aacctccgag
     1681 ccaataccgg tgcattgatt tgtttgatca tcagtttatt ggtatttatt ccgggcttgg
     1741 ttttaaaagt actccccgac ttgagcgata atcttatcgc attttatctg ttaaatatcg
     1801 ggctcacttt tgcctcattg cttgcttccg ttatcggtat atatcttgcc ggttttcaga
     1861 ataaaacatt atctggtatt tttataactc tgctggccgt ttggctcgga agttttctcg
     1921 tcaacgtaaa ggccatcgaa agcgtcgaac tgaaaacatt ggatcagagg tttagttttc
     1981 gatatcagta catagatcag gaaaaacgat taactcctga agaatcaccc atcatcatcg
     2041 ttgacgtgag cgatcaagcc ggtgaagaag ttcgtgatcg ctatccttgg ccacgcacgt
     2101 attacgccca ttttatccgc aatatgaaac gcgccggcgc taaagtggtc ggaatagata
     2161 ttgtgtttga tgccccggat tataatggac ccgaaaaaga tgatgatctt gctgcggcca
     2221 tcaaagaatg tggtaatgtc gtgttggccg gcaaagtcat taaaggtgat gtagcttccg
     2281 gcggtggaag catcggcaca acagccaaac cgcttcctaa atttctggat gaagggatcg
     2341 gaaaatgggg ttttgtcggt gtaaccaatg atattgacgg tttttggagg caatatctgg
     2401 tcgaagaaaa aacgggcgat gatgaacacg agcaggtttt tcactcattt ggcgtcgaag
     2461 ttttacgcaa atattatgat atccctgatt cttcaaaatt catcaacaat gaacaatttt
     2521 ttcagttcgg cgacaaagtt atacgtaagt ataaacccta ctcttttttg attaacttcg
     2581 caggccctaa agctacattc ccctataaat cgttcgaact tacggtggac gatgcagcat
     2641 ttgatctcaa agaagggcgc gatctggatt cgtttgatga tgaaggcgat cccgatctag
     2701 ggatcccccc cggactgctt aaatccggtt tctttaagga caaaatcgtt cttctcggtg
     2761 cgacgatgga agagttgcac gacgtgtttt ccgtaccgat cagtgaagtg cgcacttccg
     2821 atggaaaaaa actagaaacc ctgatgcccg gagttgaaat ccacgccaac gcaatttata
     2881 ccattatcaa agataactat gttaccatac aggcggattg gattcgtgtt ttaattgtaa
     2941 cgttgagcgc atttgttgtg tttctcttgg tcagtcgcct taataaaccg ttactggcat
     3001 tgccgatttt attactgatc attgcatcgg ttatcgcctt agcattttat ttatttgcaa
     3061 aacataacat tcacatgcaa ctcgtgacgc cggtattggc gatcatgttt acctatgtcg
     3121 gtaatgtgat ctttcaatat ctcggcgagc gtaaagaaaa agcacagatt cgtaatgcat
     3181 tcggtcgtta cttgcctgaa aaagtagttg cgcagcttgt agagaatccg ggcctgctca
     3241 agctcggcgg tgaagaacgt gttctttcca tccttttctc cgacgtggcg ggttttacga
     3301 cgatttctga aaaattgact ccggttgagt tggtacactt gcttaatgaa tacctgactg
     3361 caatgaccaa tattatttcc aaatacgacg gcatcattga taaatatgaa ggcgatgcga
     3421 tcatggcgga gtttggcgca cctttgcaag acgatgaaca cgccgccaaa gcatgttatg
     3481 cggcgttaga aatgcaggag aaactcgtcg aaatgcgcgt caaatggaaa cgtgaaggcc
     3541 gccctgaact taaagcgcgt gccggcatca attccgggaa cgtcgttctc ggtaatatgg
     3601 gttccgaatc cgtttttgat tatacggtta tgggtgataa tgtgaacctt gcctctcgtc
     3661 tcgaaggcgc aaataaagaa tatggtacgt acatcatgat cagcgagatg acacttcagt
     3721 tcgtcaaaga acaggttatt acccgcgaac ttgatatcct gcgcgtaaaa ggtaaagcca
     3781 aaggcgtcag agtatttgaa gttatcgccc gcacatcaac gggtatcacg gcaaatatga
     3841 aaaaaactat cgaaaactat aataaaggcc tcgaactgta tcgtcaacag cgttgggatg
     3901 aagctatggt gcttttcaaa acggcactca ctactaatcc ggacgacggt ccttcgcaga
     3961 cctatatcga acgatgtgaa gagttcaaac tcaatccgcc tccggaaaat tgggacggta
     4021 tttttgaaat gaagacgaaa taattgacaa tagtttttag attgattatt atggggcgtc
     4081 caaaagccca atctgaaatt tctatttcta aattcatata tcgcactatg aggagttatc
     4141 catgatcgaa cggagcaatt tttcaaagga caagtctatt gatcttgatc aacccgaaag
     4201 tatcattgat tggttttggg ttttctgggg ttgcgtaata ggtattgtta tcatcggcgc
     4261 tttcacgttt atcacatcta ctaaatacaa tccggacgtt gtaaaagccg taccgatgtt
     4321 gatcggcgga cttgctttcg ccgttaccgg tatgatttta ggtcattact ctcccggtca
     4381 tacagtcaaa gaagctgctg tagccggatt agttattccc atcatcggct ttgtgctctc
     4441 aggtatggga tacggcccca catttattgc cgaactcagc atatttgaaa aaatcggtgt
     4501 cgcactggcc ggtatgctga tttgtcaact cggcggttgg gtcggtgaag aattggaagg
     4561 ttatgaccat cccacgaaat ggctacaatg gcattggatt atcgttgccg ttgtgatcgg
     4621 ctttatgctc aatagttttg tgattttctt cgtcggtttg atttccctta cgccggtacc
     4681 tgtttttatg gcgttgagcg tatttgcagc cggaattgta gcaggttata agtcaccagg
     4741 acatacggaa aaggaatgca gtattgcggg tgctttaacg attctattgg attacttgtt
     4801 tattactttt gcattgggca tcgaagctaa agattttggt atcgaatccg gcacgatggc
     4861 catcatcgta ttagttgctc tcgctgttgc cggcgggtta ggccttttgg gcggatggat
     4921 cggcgaacgt atgcagattc aagaaaaaga attaaacaac tgatacctaa aattatagtt
     4981 ccaaatgaaa aaaccgaatg acagcattcg gttttttcat tttatatgca tgctttagtt
     5041 ctacattatc gtccttttcg tctttgccgc atgacatcaa aaaagacaat gcccgcagcc
     5101 accgaggcat tgagcgattg gatttggccg ctcatcggga tgcgaatcaa aaaatcacag
     5161 tgttcggcta ccattttacg catcccttta ccttcactac ctataacaat accgacagca
     5221 tcacgtacgt caatcgcatc atagtcgcga tctccgtcaa gacttgtccc gtaaatccaa
     5281 accccgcgtt cctgtaattc ttcaagcgct tgtgtaagat tagttacttg tacgacggga
     5341 atatacgcgg ttgcgcccgc agatgttttt acaactgttg cgttaacttc tgccgaacga
     5401 tgtttaggaa tgactacacc gtgaaagccg gcgcattcgg cgctgcgaat aatcgctccg
     5461 agattatgcg ggtcggttat ttcatccaaa atagcaacga gcggtttctc tccggcgacg
     5521 gcggcacgtt caaatataac gtccatttcc acatattgga attctttttg atcaagcaat
     5581 accatggcgc cttggtgatt attttttccg ccgatcatat ctgaaaaata ccgcgcatcc
     5641 atggttcgaa cacgaatttg ttttcttttg cagacgttaa gaatttcatc aatggcgtcg
     5701 ccatgagcgc cgatttgcag tattacttct tttacaggaa ttccagattt aagcgcttcg
     5761 aaaacaggtt gtcgcccgaa aataatatta gttttcatta attttccgtt taaaactact
     5821 cttcattgtt aacttcaacg aatgaaaaaa agatcggatg gtcaaccaac ccatcgtttt
     5881 ttcagttacg gtttgatata tcaaaacaca cccaagcatg cctaaaaaga aattcataaa
     5941 ccacattgca aaaaaaggat tcattagtcc gcgatccgcc agatcctctc cgagtattaa
     6001 acagaaataa tatgcaataa agaaaaacaa actaatccct gcggcgagac ccaaattacc
     6061 acgcttggct ttcacaccga taggcgcacc caataacaca aaaatcaaac atgccatcgg
     6121 tatcgaatat tttttattta cctcaaccat actctgatcc atcagtcgct gatagctcga
     6181 aacggtactg atgtttcccg aaacctgatt gctgatcgcg gttaacggca tttctacaga
     6241 atctaccggt attgcagact tgctcatatt gggtcgtcgc cccaaactcg gatctaaact
     6301 gcgtaaagcc gatggttgtg attcagccac cggtaccgta tgtttcagag tgtctttcaa
     6361 ataagtttgt atagtttgtt tcagagaatc tgataaaaca taatccgtat atgggacata
     6421 agccgtatcc aacgccaaac gtagattcca atccatgaaa taatcattga tcgcatcaag
     6481 ccaactgaat tccacacaat atttttcgac taatttgcgt gcgcggggat ccaattgcgt
     6541 aacggattcc ggtttttttt caatattttt taaaaccaac cccatctgcc tttcacgttc
     6601 tttgcgatgc cgtgctactt cttccatgag ctgatcaata tttttttcgc gatcgtttct
     6661 aaaggactca tcacttgttt ttagcgatag gttttcaacc gcaacgataa ctttgtattt
     6721 ttgaaaaatt atgcgttgat attgatcatt tttgccctga tggtattcgt gtatttcacc
     6781 ttcttccaac tccaatatca tattatcgtt ggattcttcg atcgctatag atccgcgctt
     6841 ggccgttatg gttcgttgtg cattacggtc ggatttatca aatatcgtca cgccataaat
     6901 tgtcgatccg agttcatctt tattttcaac gatcatgcta aaattttcaa tgccctccaa
     6961 aaacacaccc ggttcaatag aaagcgtcgg tctcttagcc tggatatttt tttttaaaac
     7021 gcgtgcattg tgattagcca acggcagcac ttcatcatta aaccaaaaaa caccgtaggt
     7081 aacaatacac gcaacgagaa tgacaggaaa tatcaattta aagaaattaa tacccccggc
     7141 cttaagggcc gtcacttcat tgtcggaggc gaggcgaccg aacgccatga tcgttgcgac
     7201 gagaatagac atcgggacga ccaatacgat gatacccgca agattgtatg caatgaactc
     7261 taaaatcacc gtcatgctga tgcctttgcc aataatatag cgcatcgctt gcagtacaaa
     7321 attgagtaaa agcatggatg tgataagcgc aagcgcaaag aaaaacggtc cgatgtgttc
     7381 gcggatcaca tagcgggaaa cgataaacat gcggtgacgt ttcggtatta agatttgagg
     7441 agatttatct gatcgcggag tttggcggcc aactcatagt tttcagactc aacggccttt
     7501 tgaaggtctt tttccagttt ttcaagcggt gtcatggaaa gttcggatgc ttccgaagcc
     7561 ggaacttttt ttgtttgttt gacatacgcg ccggattgaa catccacatc caatcccgca
     7621 gcttccatca ctttttcttc cacaaaaatc ggagagccga cgcgtatcgc tatcgcgatc
     7681 gcgtccgacg ggcgcgaatc aatcacttgt tccccgacag aattactgcg gatatgaatc
     7741 gaagcaaaaa aagtattttt atttaattcg gtaatgacaa ctttgtcaac ttcagcgtcc
     7801 aaggcgtcta aaatctggac ggctaaatca tgagtaaacg ggcgaaaagg tttgataccc
     7861 tgaagttcaa gtgcgatcga atgcgcttcc ggagcgccga taacaaccgg taacatgcga
     7921 cctttttcag ggccggtttc taccaattgc aggatatagc cattactctg ctcatcctca
     7981 ataatccatt ttatttcgac ctgcaattcc attacaattt ccgtttctgt tctagaataa
     8041 gttaatatac gacttaagga aaatcaatta tttttaacgg tgaaatcgga ttcgttttcc
     8101 tgcccgacaa acagcagatc gggtatttca tcgatgatac cgtaataaaa gcaataagaa
     8161 taatattcct tcagcccttc aagttccgct ttcccaagtt tgaattgtat attctccgtg
     8221 agataattgg cataaaaatc aggttcaaaa cctcgcgcgc gttcctgact gtaaactcgt
     8281 gaaatctccg taatgtgatc caggcccgca tcgcgggaag ctgtcagtgt aagaatatca
     8341 tccgtattca atgcgtcttc agcggcggcc caaaacgaat acacaaatgg taatccgtcc
     8401 gtcatatcac cccactcttc tcccagatca attttgttat cccatgtatc gtataactca
     8461 agcgcattat caccgatgat caaggccgca tcggcctttg acagcataac ttcgacttgc
     8521 ggcggcatag gaataaattc agggtgaata tcaaattttt cacgcagaat gatatccagc
     8581 aaccccactg atgtacggct gctaatatcg acggcaacgg tattaatatt ttcaagattt
     8641 cgattaaaaa aaagctccac acttttcaca aattgatgag aaatcacacc gatatcgggc
     8701 acgattcgaa ggtttcgatg gccgcgaata cgcgaatatt caattgacgg tatcaatgcg
     8761 agatcgactt ctttgttttt aagtttatcc gcacagaccg aaggaatagc gtattgaagt
     8821 tcaaaccgcg ctgccgcggc ttgctgcaaa ccataaacga taggtctggt atttagatac
     8881 tctacagcac aggttttaat tttttgcaaa cgtcttattc ctcattttca tcacgatctt
     8941 catcttcttc tgactcaccg acatcggcgt cctcagacag ctccatatca ggtaattcga
     9001 tgtctttaca aacatccaaa gcgtcctgca atttatcatg aggaacgagg attttgatct
     9061 ccgataagtc tcctacccac accgtattga cactgtcttt ttgactgaac acggtatttt
     9121 ctataccggc cgactgtagc tggtcactga tcagctccgc ttgatgctca taggaaaccg
     9181 ttttaacaac ggcccaacct tcgattaatt cactcataac aacctccgtt ttatttgggt
     9241 tgcagtactc cgatccagta ttccgcgctt tctttccatc gtttatcgtt ggccgctttc
     9301 cgataatctg ctaatgcttc ctcgattcta ttcaaaagtc gtaaagcttc agctcgttca
     9361 aaaaaaattc cgtactgcca tttttcttct tctatatttc cggcttggtt tatcaatcgt
     9421 aaagctcgct ccggttcttt ggcttcattg taacaattag atagttcata caaagaagcg
     9481 gcatgcactg gatgatgact aactatctgt tctaaaattg ccacggcttc atccgttcga
     9541 ttcagttgat cctttagaag cttcgcttta atgtaagaaa tttcaggatg aaccgcatta
     9601 tttttcaaaa aatcattaca agtttcaata gctagcgaat aatttttaac atccgagaga
     9661 agccatactt tgtattgaac agcctttgcg gttatatttg tatttgcatt cggaagatga
     9721 atggctaaat cataatacgc tatagcgcgg ctgatttcac cggtacgccg gtaagccaag
     9781 ccgagattta cgtaagcttt ttggtaagac gagtcggcgg taatcgcttt atgataaatg
     9841 gatatcgctt cgttccagcg tttagcttta cttaatctat tcccctcttt gtaataaccc
     9901 cgcgcctgtt ctcgcgctac gcgacgggat tgattgagac tatcggtttc tttcctcttt
     9961 tgtttccaat tttcaagttg cactgtataa tcatcgtcat tgtcatcctg ttcttgccca
    10021 aaagcgatgg gacacgtact aaataacaaa atgatataaa acaaaaaagg cgccgacttt
    10081 gtcatcgaat cgcctttaaa taaatttgga ttcataagtg cggaaggcgg gatttgaacc
    10141 cgcacgccta ccggcgccac cccctcaaga tggtgcgtct gccaatttcg ccacttccgc
    10201 ataaattagc gcacgaaata tacgctaatt ttattttttt gcaataaaaa aagctgcaaa
    10261 acccgtaaat cttacgaatg ttttgcggtt cttagttttg cggtgtagga agcgcaggcg
    10321 tagactgtcc gccaccttgt tccagttctt tttctataat accctgctcg gcgccaccat
    10381 cgcgcaatcc tttgatatta aggctcacac tgagtacggc aaacaaaatg gcaagtacaa
    10441 ccgtagcttt gacaagggtg tctgcagctt gacgtccgct aagaatagtt gaagcaccac
    10501 cgccgagacc tgcaaggccg ccgcctttgc tggattgcat aagaatcgca ccgatcagca
    10561 aaatgctgac aatgatgtgt aaaacaacaa gaaacgcaaa catggattta ctcctgagta
    10621 atgatgaatt ataattgaat tatatttttt cggctgcatg tatgatcgcg gcaaacgatt
    10681 cggcttcgag gctggcgccg cctacgagag cgccgtcaat atccacacaa cctaataatg
    10741 cggcagcatt atcgggcttc acgctcccgc
//
LOCUS       DAOQGF010000107        10490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2957_length_10490_cov_6.144514, whole genome shotgun sequence.
ACCESSION   DAOQGF010000107 DAOQGF010000000
VERSION     DAOQGF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10490
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_10490_cov_6.144514"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..887)
                     /locus_tag="PK937_12660"
     CDS             complement(21..887)
                     /locus_tag="PK937_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH33469.1"
                     /translation="MKYLILFLLPAFLCAQNNRIRLESLPLDDGLSYQSVYALYQDSR
                     GYLWIGTISGLIRYDGRNNTVFRHEPENPHSLSHDDVVAIYEDEFKKIWIGTYGGGLN
                     RYNPETGVFTRYLNDSTDTKSISDNVILSITGDKNGRLWIGTRTGGINVLNITLPDSG
                     FTRFTRDPKKPRRTAPQAPQKLYTSSDGRIWIGAFGRGLVTYDQSENGFRYFPLTRNS
                     GDTVRFYNAYDIVEINNLLWISTDVGLKTFDLKVDQWIESTFPTKATCDSLSAFRLAV
                     DRINNVLWVLNP"
     gene            complement(924..1811)
                     /locus_tag="PK937_12665"
     CDS             complement(924..1811)
                     /locus_tag="PK937_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33470.1"
                     /translation="MRFFILILMIPIVNFAVSQTQVDIPEKAEAAYKASDWATSVKLY
                     LQLCKSQPGESKFWNRLATSYYQLGAYKNASETYRQYLSLTQDPVGMYNAACSFALSG
                     EKDTAWYWLEKATQSGWFTSLQVRHDNDLKSLHSRKFDDLVISLDRAARPCEYDSLFR
                     QFDFWIGEWDVFNLAGQKAGTNTIQKLTDGCLLFENWTGSLGGSGKSINFYDKQKKLW
                     RQIWIASNGNVSDFYGQYSEGVMDFYGESVSANGSIGKNRLRFFKIDSDTVRQFAERS
                     DDNGKSWQVTYDFRYVRKK"
     gene            complement(1897..2424)
                     /locus_tag="PK937_12670"
     CDS             complement(1897..2424)
                     /locus_tag="PK937_12670"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNH33471.1"
                     /translation="MREIRWIAMVSLFFLTGSVVWGQTENSEPPDDAALKHAVENKIQ
                     GIVWYGARDYVNVEVSEGTVILNGWVSGYWKTDKIQKAVEQVPGIKEITNEITVSNGS
                     DELAGRALSAIYRDAMFRKYALAADLPVHVIVQNNSVILAGKVMVESERRRAEFLVDA
                     HTAAAAIDNRLVIID"
     gene            complement(2481..3200)
                     /locus_tag="PK937_12675"
     CDS             complement(2481..3200)
                     /locus_tag="PK937_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNH33472.1"
                     /translation="MKRFHCIKRVVVFSFIMGLLGLLSCDDDSAKDTDDGTANNEPGL
                     VAHYPLDGNADDVGNSHSDGVVSGAVSANDRHDQVGKAMYFNGNSDFISVNDTSNFQF
                     DDSLTVTAWVRTDSLKSQIVVRKGGGLAVPVFELYLAGNGDRGFRIGTNFTYKSGGYP
                     LQTWMHLAGTYDGESMVFYINGSPVDTLQKSNALTSDKNLLLIGTRTGLNADTFAGRL
                     DDIRIYSRSLSLSEIAAMAAE"
     gene            complement(3347..3700)
                     /gene="erpA"
                     /locus_tag="PK937_12680"
     CDS             complement(3347..3700)
                     /gene="erpA"
                     /locus_tag="PK937_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNH33473.1"
                     /translation="MTETMEAVNDVVITPRAIEEIGSIMREQSIPENYALRMGVQGGG
                     CSGFQYSLAFDETISENDTILEFGAVKVAVDYKSMLYLKGVTLDFQDGLNGRGFVFNN
                     PNAKKTCGCGSSFSA"
     gene            3913..4158
                     /locus_tag="PK937_12685"
     CDS             3913..4158
                     /locus_tag="PK937_12685"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNH33474.1"
                     /translation="MTSNKLKTVAQLGCGECAVICKISGEHLENDSTCIRLMEMGFTP
                     GQEVKVIAKSPFRCPMAVSVRGTVIALRKNEAECIHI"
     gene            4155..6203
                     /gene="feoB"
                     /locus_tag="PK937_12690"
     CDS             4155..6203
                     /gene="feoB"
                     /locus_tag="PK937_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNH33475.1"
                     /translation="MTDSVLPSDCHTPSSNSVSGTSSNASFRITLIGQPNSGKTTLFN
                     ILTGSHFKTSNYPGTTVEFLVGRLHERFKCDAQVIDSPGINSLTPQSLDERVSVDSLF
                     LDPNYRHPDLVLVTADATQLSRQLYLVKQLIDAGFPVIVALTMNDLLQKRGFSIDTTL
                     LSERLGTPVIKTYGHTENGVAELAEALRGFFAVPQKYILPPIPKLQSESTVMELYRIT
                     ESIEKEVIRPFSVKPDIHAINHAVFNTRTIEPDETSLKLDRIFLHPIWGMFFFVFFMT
                     LIFTSVFWLAQPMMDVISEGFDVLANLSKSWLPSSHWLGNLIADGLIAGMGTVMTFVP
                     QIVILFLLMGMLEDSGYLSRGAMLIDKPLSKIGLNGRSFVPMLSGFACAIPATMAART
                     IPNQRERLLTIFIIPLMTCSARLPVYALLLGFLMPSDRPWLSGLILSGIYFVSLINGA
                     LVAGIVSRFIKEKTPSSFMLELPAYRKPIIRFILRNTFQRSVSYIQNAGTTIILCSII
                     IWGLTYFPNANPEITDEAKASGNVEQWIAGERTSSSYAAMVGRWIEPALMPLGWDWRV
                     GVGMISAFAAREVFVSATALTFHIAEDDEDNMQQSLINSMRTARHEQTGKPVFTVSSV
                     VAIVIFFMFALQCTSTLAVVRKETGSWKMPLIQQVAFTTIGYGLGWLAVVLLNAVGIA
                     "
     gene            complement(6210..6398)
                     /locus_tag="PK937_12695"
     CDS             complement(6210..6398)
                     /locus_tag="PK937_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNH33476.1"
                     /translation="MITRCICFDKEFEELKKVAAAYRCKTLEELQERVLVGVRCKLCH
                     PYIRRMLATGQTRFEVNE"
     gene            complement(6398..6949)
                     /locus_tag="PK937_12700"
     CDS             complement(6398..6949)
                     /locus_tag="PK937_12700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNH33477.1"
                     /translation="MKIYTKTGDDGTTGLFGAGRVQKDATRIESYGTVDELNSVMGLV
                     QCHSEDDELDMVYAGIQNELFILGADLATPMDVKSEYIIRIKAENTLQIEKRIDSFDV
                     RLPELTNFILPGGTQASSYLHLARNVCRRAERLTVKLSKEEVINEHALTYLNRLSDLL
                     FVLARWENAKAGIADTMWKKPVD"
     gene            complement(6955..7914)
                     /locus_tag="PK937_12705"
     CDS             complement(6955..7914)
                     /locus_tag="PK937_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33478.1"
                     /translation="MGCNESDDSFSTIPSGLSSVWIINEGNYGTNNASITLLDTLFED
                     TTFSNATGSYLNGSAVHSAVFTDSTTWLMMTGSDKISILHRRTLKAMRTITGIKSPRN
                     GVLYQNNVYVTSYFDSLVIAYNAADYTETNRYKLSHRPDEITALNGLLIVSNSRSPDG
                     VLSKDSVISLIDITMSDVNAYVIGSPVVSVCANNPAGKVYIAYTGKSSSAGKIVEWDP
                     LTKVITSEVVSGINPTFVRMHDSVLVYNTRSNSAIIRYDMKTDIKITLAGSYFVGAPL
                     GEDILAADAQDFLRNGYLYWIRKDGTIRKRYPVGITPSSILQP"
     gene            complement(7994..8551)
                     /locus_tag="PK937_12710"
     CDS             complement(7994..8551)
                     /locus_tag="PK937_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33479.1"
                     /translation="MTMRTSNMLRFVPTLVTVLLIVGYRLMPHPWNFVPVTAMAIFGG
                     IYLNRSLAYGLPLASMIVADLFLGMDWTSPVVYASILVSSWIGTRVRNVYENKKQFAV
                     AVGGGTLASSVLFYLTTNFAAWLVIPEYTKDISGLIQSYIMAIPFFKNALAGDMFFIV
                     VFVGVYEAIHFIVTKLSRQSAPIHG"
     gene            complement(8649..9689)
                     /locus_tag="PK937_12715"
     CDS             complement(8649..9689)
                     /locus_tag="PK937_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33480.1"
                     /translation="MRYIGIVLSFTMLLSCEERISDPKSGTITEGIFILNEGSFGAGN
                     GDVSLYRPDSADVINNLYLSANGKSAGDIVQDMLILDTMAVISINNSNKICFVRLKDF
                     TLLSTIGTLQPRMFVKGDDDKVYLTAWDGKLKTLDLAQKRVTDSIAIGTAPESLFLDG
                     NDLYIANYGYGNDNTVKIYNTVSKSITKTIRVGYGPRAFSKSSGDNKLFIACEGNQYA
                     EIPVPGGIYVVDLNTETMVDSLVSLPSSTGAKSFYPGRIAVNENMGYFISGFYGDIQK
                     FNISNWTIIDSIKGSFYNVVLNPDVDSDVYATDASLVPGKFEIFDQDGQVKQSFTVGD
                     FPSGMVFYNKNK"
     gene            complement(9709..>10490)
                     /locus_tag="PK937_12720"
     CDS             complement(9709..>10490)
                     /locus_tag="PK937_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH33481.1"
                     /translation="SLRLSPAIRWDKFSDFGQVWSPKAGMNIAYEKSGYYTSLRASWS
                     RNFRAPTLNERYWKDTDASGNPDIKPERSTTWDLGGRFGGSQAKHSFEIEYTWFVMRA
                     HNQIAWNLAGIIHPENIRKSSSRGWEIAMKYEKEQMWETKLELSRAKAVDTSDPDNVR
                     RIPYNPLYTTSLSNYAMFKDFTIGHTTHYQSQRYTDAVNNSVIPSYWLTDAIVSWHKQ
                     IISYDVQLSVIVKNIFDKAYESVAQYPGLAREYVVGTKITF"
BASE COUNT         2973 a         2413 c         2007 g         3097 t
ORIGIN      
        1 ccggccggtc ggtcggttgg ttaaggattt aaaacccaca acacattatt tattctatcg
       61 acagctaatc taaaggccga taacgaatcg catgtcgctt ttgttggaaa agttgattcg
      121 atccattgat caacttttag atcaaatgtt ttcaacccta catccgttga aatccacagt
      181 aaattattaa tttctacaat atcgtacgca ttataaaacc tcactgtatc gccggaattc
      241 cgagttaatg gaaaatatct aaaaccattt tccgattgat catatgtcac caatcctcgc
      301 ccaaatgcgc caatccaaat acgaccgtcg gatgaggtat ataacttttg gggtgcttgt
      361 ggcgctgttc ggcgcggctt tttcggatcg cgcgtaaatc gtgtaaaacc cgaatcgggt
      421 aaagttatgt tcaatacatt gattccgccg gtgcgagttc ctatccatag ccttccattt
      481 ttatcaccgg ttattgataa aatgacatta tcgctgatgg attttgtatc cgtcgaatcg
      541 ttgaggtatc gtgtaaatac accggtctca ggattgtatc ggtttagccc gccgccgtaa
      601 gtaccaatcc atattttttt gaattcatct tcgtaaatag ctactacgtc gtcatgagac
      661 aaagagtgtg gattttccgg ctcatgacga aaaacggtat tattacgccc gtcataacga
      721 atgagaccgc tgatcgttcc gatccatagg tagcctcgag agtcctgata aagtgcatat
      781 acggactgat acgataaccc atcgtctaac ggaaggcttt ccaaacgaat acgattgttc
      841 tgcgcacaaa gaaaagccgg taaaagaaat aagattaaat atttcattag acatattcga
      901 tttgtgatcg gcgggactca acgtcatttc ttacgaacat aacgaaaatc ataggttacc
      961 tgccagcttt ttccgttatc atcggaacgt tccgcaaatt ggcgtactgt atcggaatca
     1021 atcttaaaaa aacgtaatct attttttcct atactcccat tcgccgaaac ggattccccg
     1081 taaaaatcca ttacaccttc ggaatactgc ccataaaaat cagaaacatt gccattgctt
     1141 gcgatccata tctgtcgcca tagttttttc tgtttatcat aaaaattaat actttttcct
     1201 gaaccaccca gtgaaccggt ccagttttcg aacaataaac aaccatcggt taatttttga
     1261 atcgtattcg tacctgcttt ttgtccggcg agattaaaca catcccattc accgatccaa
     1321 aaatcaaatt gacgaaacaa tgaatcatat tcgcaaggtc ttgcagctcg atctaatgat
     1381 atgactaagt catcgaattt cctggaatgg agtgatttca aatcgttatc atggcgtacc
     1441 tgcaatgacg tgaaccatcc gctttgggtc gctttttcaa gccaatacca cgctgtatcc
     1501 ttttcgccgg ataaagcaaa tgaacaagcc gcattataca tgccgacagg gtcttgagtc
     1561 aaagataagt attgacggta tgtctcagac gcgtttttat atgcccctag ttgataatac
     1621 gacgtcgcga gacgattcca aaattttgat tcacccggtt gactcttgca caactgcaaa
     1681 tataatttta cggaagttgc ccagtctgaa gccttataag ccgcttccgc tttttcagga
     1741 atatccactt gtgtttgaga aactgcaaaa ttgacgatcg gaatcatcaa aattaaaata
     1801 aaaaaacgca taaacgcctc ctgaggtata acttcaaaat acgtttatgc gtttttagat
     1861 caactcacca atagtggtga ttttagcaaa ttaaaactag tctatgataa ctaaccggtt
     1921 atcaattgcg gcggcggcag tatgagcgtc cactaaaaac tctgcacgtc ggcgttcgga
     1981 ctcgaccatc actttgccgg ctaaaataac ggaattattt tgcacgatca catgtaccgg
     2041 aaggtcggca gccaatgcat attttctgaa catggcgtca cgataaatcg cacttaacgc
     2101 gcgcccggcc aattcatctg atccattgga aacggtgatt tcattcgtaa tttcctttat
     2161 accgggtacc tgctctaccg ctttttggat tttatcagtt ttccaatacc cactaaccca
     2221 accgtttaat attactgttc cctccgatac ttcgacgttt acatagtcac gtgctccata
     2281 ccatacgata ccttgaattt tattttctac tgcatgcttc aaagcggcat catccggagg
     2341 ttcagaattt tcagtttgac cccacacgac acttccggtt aaaaaaaaca gagacaccat
     2401 tgcgatccag cgaatttctc tcataacgcg gctcctttcg tcattttatt gttagacaga
     2461 gtaagcgtta agaggtttaa tcattccgcg gccatcgctg caatttcgct caaagatagg
     2521 gagcggctat aaattctgat atcatccaga cgccctgcga atgtatctgc gttaagacct
     2581 gtacgcgttc caatcaatag cagattttta tcgctggtca atgcattact tttttgtaac
     2641 gtatcaaccg gtgaaccatt aatataaaaa accatagatt cgccatcata agtaccggcc
     2701 aaatgcatcc aggtttgtaa aggataccct ccgcttttgt atgtaaaatt tgttccaatg
     2761 cgaaatccgc gatctccatt accggcaagg tataattcaa acacaggaac tgctaaaccg
     2821 cctcccttac gaacgacaat ttggcttttc agtgaatctg ttctcaccca tgccgttacg
     2881 gtcagcgaat cgtcaaattg aaaattagaa gtatcattga cactgataaa atcagagttc
     2941 ccattaaaat acattgcttt tccaacttgg tcgtgacgat catttgccga aactgcccct
     3001 gaaacaacgc catcgctatg ggaattgcca acgtcatctg catttccatc taatgggtag
     3061 tgcgccacaa gccccggttc gttatttgca gtaccatcat ctgtgtcttt agcgctatcg
     3121 tcatcacacg ataacaaccc taataaaccc ataataaaag aaaaaactac aaccctctta
     3181 atacaatgaa atcttttcat ttttccctca tattgttttg tttgctgatt caaacaatat
     3241 ggatggttat cgcgatcaga gcacctgcat tatgatgtag tacgcatcaa aaaacaaaaa
     3301 acccgataca ctgaagtatc gggttttata taaaactact ggaaatctat gcagaaaatg
     3361 aactaccgca gccgcacgtt tttttggcat tagggttatt gaaaacaaaa ccgcgtccgt
     3421 ttaacccatc ttgaaagtca agagtgacgc ctttgagata taacatactc ttgtaatcaa
     3481 cggcaacttt gactgcgccg aattccaata tcgtatcgtt ttcagaaatg gtttcatcaa
     3541 aagctaacga atattgaaaa ccagagcaac cgccaccttg aacacccata cgcaaagcat
     3601 aattttcagg aatactttgt tcgcgcatga tcgaaccgat ttcttcgata gcacgcggtg
     3661 taatcaccac gtcgttaacg gcttccatag tttctgtcat gatattatct ccttaaacca
     3721 ctgtttgatc tataattagg tgttttcaat aaaactaatg tagaataaaa aaattccctt
     3781 gtcaagcatt ttcctgacgt atatcattgt ttctcttgac tggttgactg tattattcta
     3841 tattcttttc attaagattg agacgcagtc tcaatcttaa tgaaattatt aaacattaaa
     3901 gaaactttag ctatgacgtc gaacaaattg aaaactgtag cacaactcgg atgcggcgaa
     3961 tgtgctgtta tttgtaaaat cagcggcgaa catcttgaaa acgatagcac atgtatacgt
     4021 ttgatggaaa tgggatttac gcctggacaa gaagtgaaag taatcgccaa gtcccctttt
     4081 cgatgcccta tggcggtttc tgttcggggt acagtaatag cgcttcgtaa aaatgaagcg
     4141 gaatgcattc atatatgacg gattcggttt tgccatcgga ttgtcatacg ccatcttcga
     4201 actctgtttc gggcaccagc agcaacgcta gctttcgcat tacacttatc ggtcaaccta
     4261 actccggtaa aaccacttta tttaatatcc ttaccggctc acattttaaa acttctaatt
     4321 atccgggcac gacggtcgaa tttcttgttg gtcgtttgca cgagcgtttt aaatgtgacg
     4381 cccaagttat tgattcgccc ggtatcaaca gcttaactcc gcagtcgctg gacgaaagag
     4441 tttctgtcga ctcgttattt ttagatccca attaccgaca tcctgacctt gtcctcgtta
     4501 cagcggacgc cactcagctc tcccgccaac tttatctggt taagcaactt atagatgcag
     4561 gctttccggt catcgtcgct cttacgatga atgatttact ccaaaaacgc ggtttttcga
     4621 tagatacaac tttgctatcg gaacgacttg gaactcctgt tataaaaacg tacgggcaca
     4681 ctgaaaacgg cgtggctgaa ttagctgaag ccctacgtgg tttttttgca gtcccgcaaa
     4741 aatacatatt acctcctatc cccaagctcc aatcagaatc aacggtaatg gaattgtacc
     4801 gaataactga atccatagag aaagaagtga tacgaccttt ttctgtcaaa cccgacatcc
     4861 acgctatcaa tcacgctgta tttaatacgc gtaccatcga accggatgaa acatctctaa
     4921 aattggatag aatttttcta cacccgattt ggggtatgtt cttttttgtt ttttttatga
     4981 cgcttatttt tacgtctgtt ttttggttag cccagccgat gatggatgta atttcagagg
     5041 ggtttgatgt actagccaat ctctcaaaat cttggctccc atcatctcat tggcttggta
     5101 acctcattgc tgacggatta atcgccggta tgggtaccgt gatgaccttt gttcctcaaa
     5161 ttgtaattct ttttttactc atggggatgc ttgaagattc cggttactta tctcgcggcg
     5221 ccatgctcat tgacaaaccg ctttccaaga tcggactaaa cggtcgttcg tttgtaccca
     5281 tgctttcagg ctttgcttgc gcgataccgg cgactatggc cgctcgtact atccccaatc
     5341 agcgggaaag actgcttacg atttttataa taccgttgat gacatgcagc gcgcgattac
     5401 cggtgtacgc attgctttta ggtttcctga tgccctcgga tcgtccttgg ttgagcggat
     5461 tgatcctatc cggtatttat tttgtgagcc ttatcaatgg tgcactcgta gcgggtattg
     5521 taagtcgttt tattaaggaa aaaacaccgt ccagttttat gcttgaattg ccggcttacc
     5581 gcaaacccat tatacgattt atactgcgta atacctttca gcgttcagtt tcatacatcc
     5641 aaaacgccgg tactacgatc atattatgct cgatcatcat ctgggggctt acctattttc
     5701 ctaatgcaaa ccctgaaatc accgacgaag ccaaagcttc cggaaatgtc gagcagtgga
     5761 ttgccggaga acgcaccagc tcttcatatg ccgcgatggt tggtcgatgg atcgaacccg
     5821 cattaatgcc gcttggctgg gattggcgtg tcggcgtggg catgatttcc gcttttgctg
     5881 cacgtgaagt tttcgtcagt gcgacggccc tgacatttca cattgcggaa gatgatgaag
     5941 ataatatgca gcagtcattg attaattcta tgagaaccgc ccggcacgag cagacgggta
     6001 aacccgtatt tacggtttct tcggtcgtag ccattgtaat attttttatg tttgcattac
     6061 aatgtacatc tacgctcgcc gttgtgcgca aagaaacagg ctcgtggaaa atgcctttga
     6121 tccaacaagt agcttttaca actatcggtt atggattagg atggttggca gttgtattgc
     6181 ttaacgccgt gggaatcgct taaaaacgat tactcattga cttcaaatcg tgtctgaccg
     6241 gtagccagca ttcgtcgaat atagggatga cataatttac aacgtacacc gactaaaacc
     6301 cgctcttgta attcttccaa tgtcttacag cggtatgctg cagctacttt tttgagctct
     6361 tcaaattctt tatcaaaaca aatgcaacgt gtgatcatta gtcaaccggc tttttccaca
     6421 tggtatccgc aatgcctgcc ttcgcatttt cccagcgtgc caaaacaaat aaaaggtcgg
     6481 aaagccgatt aaggtacgtc aatgcatgtt cattgatcac ctcctcctta gacaatttta
     6541 ctgtcaacct ttcggccctg cggcaaacat ttcgcgccaa atggaggtat gacgaagcct
     6601 gtgtgcctcc gggtagaata aaattcgtca gctccggtaa ccgtacgtca aaactatcaa
     6661 tacgtttttc gatttgcagg gtattttcgg cttttatcct aataatatat tcacttttaa
     6721 cgtccatcgg ggttgccaaa tctgctccca gaataaacaa ttcgttttgt atccccgcat
     6781 ataccatatc caattcgtca tcctcgctat ggcattgtac taaacccatg acgctgttca
     6841 gttcgtcaac ggtaccatac gattcgattc gagtagcatc tttttgaacg cgtccggcac
     6901 caaataaacc ggtcgtgccg tcgtcccccg ttttcgtgta gattttcata gattttatgg
     6961 ttgtaaaatt gaacttggtg ttatacctac cggatatcgt ttgcgaatag ttccgtcttt
     7021 acgaatccaa tataggtaac cattccgcaa aaaatcttgc gcatctgctg ctaatatgtc
     7081 ttcacctaat ggcgctccaa cgaaatagct acccgctaaa gtaattttga tatcggtttt
     7141 catatcgtat cgtataatgg cactattgct ccgcgtgtta taaaccaaaa cagaatcatg
     7201 catacgaaca aaagtagggt tgataccgga gactacttca gaggtaatta ctttagtcaa
     7261 ggggtcccat tctactattt tgccggcact agaactttta ccggtataag cgatataaac
     7321 cttaccggca ggattattag cacatacgga aacgaccggt gaaccgataa catacgcatt
     7381 gacgtccgac atcgttatgt caatcaaact gatcacacta tctttggata atactccatc
     7441 aggcgagcgg ctattggata cgatcaacaa tccgttgagt gctgttattt catccggtct
     7501 atggctgagt ttataacgat tcgtttccgt ataatcagcg gcattatacg ctataaccaa
     7561 actgtcaaaa tacgaggtca catacacatt attctggtac agaacgccat tacgcgggct
     7621 tttgatgcca gtaatcgtgc gcatggcttt aagtgttctc cggtgcagga tactgatttt
     7681 atcggagccc gtcatcatca gccaagttgt tgaatcagta aaaaccgcgc tgtgaacggc
     7741 gcttccgttc agataagaac ctgtggcgtt tgaaaacgtt gtatcttcaa acagcgtatc
     7801 aagcaatgtg atggaagcat tgttagtccc ataattacct tcattgataa tccatacact
     7861 cgataaaccg ctcggtatgg tcgaaaagga atcatcgctc tcattacaac ccatagaaaa
     7921 aaaaatcccc acggcgaaaa tgtataacat caatttcaac cgtgaggtgt aaaaaaataa
     7981 agcgctcaaa ttattatccg tgaataggcg ctgattggcg actgagcttt gtaacgataa
     8041 aatgaattgc ttcatatact ccgacaaaca ccacgataaa aaacatatcc ccggccaatg
     8101 catttttgaa aaaaggaatc gccataatat agctttgtat caagccggat atatctttgg
     8161 tgtattccgg aattacaagc cacgcggcaa aattggttgt cagataaaaa agtaccgacg
     8221 aggctaaggt acctccacct acggccacgg caaactgttt cttgttttca tagacattgc
     8281 gaactcgggt tccaatccat gagctgacta atatcgacgc ataaacaacc ggtgaagtcc
     8341 aatccattcc gagaaaaaga tcggctacga tcattgacgc taacggtaag ccgtacgcca
     8401 acgagcgatt caaatagatc ccaccaaaaa tcgccatcgc agttaccgga acaaagttcc
     8461 aaggatgcgg cataaggcga taccctacaa ttaataaaac cgtcaccagt gtaggaacaa
     8521 acctcagcat gtttgacgtg cgcattgtca taatgattac cttttgatta tacgttatat
     8581 atgattgaat gataaatttc tattcactgc agacgcgtca atatagtttg ccttggataa
     8641 ttttgcaatc acttattctt gttgtaaaat accatcccgg acggaaaatc accaacggta
     8701 aagctttgct tcacttgacc gtcttgatca aatatttcaa attttcccgg tactaacgac
     8761 gcgtcggttg catatacgtc cgaatccacg tcgggattta aaacaacgtt gtaaaaggaa
     8821 cctttgatgc tatctataat ggtccaattg ctgatattaa atttttgtat gtcgccatag
     8881 aacccactta taaagtaacc catgttctca tttacagcga tacgccccgg ataaaaagat
     8941 ttcgcacctg tagagctagg caaactcacc aacgaatcca ccatcgtttc ggtatttaaa
     9001 tcaacaacgt aaattccgcc aggcaccggt atttctgcat attgatttcc ttcgcatgcg
     9061 ataaaaagtt tattatcccc ggatgacttg ctaaatgctc gcggaccata accgacacga
     9121 atggttttgg taatcgattt tgacactgta ttgtagattt tcaccgtatt atcattgccg
     9181 taaccgtaat tagcgatata aagatcatta ccgtcgagaa acaaagattc cggcgctgtg
     9241 cctatcgcaa tcgaatccgt cactcttttt tgtgccaagt ccagggtttt gagttttccg
     9301 tcccatgccg ttagataaac tttgtcatcg tcacctttga caaacatacg cggctgtagc
     9361 gtacctatag ttgataaaag cgtgaaatct ttcaaccgca cgaaacatat tttattcgaa
     9421 ttattaatag aaataacagc catcgtgtca agaatcagca tatcttgtac aatatcgccg
     9481 gcagatttgc cattggcact caagtaaaga ttattgatca catccgcact atcagggcga
     9541 taaagagaaa cgtcaccatt tccagcgcca aatgagccct cgtttaaaat aaatataccc
     9601 tctgtaatgg ttccggactt agggtcagaa attctttctt cgcacgataa tagcatcgtg
     9661 aaacttaaaa caatccctat gtagcgcatg ctttttttct ccaatgtgtt agaacgttat
     9721 tttagttccc acgacatact cgcgcgcaag cccgggatat tgcgcaacgg attcgtaagc
     9781 tttatcaaaa atatttttta cgattacgct aagttgtaca tcataactaa ttatttgttt
     9841 gtgccaagac actatcgcat ccgtaagcca ataactcggt attacactgt tattgacagc
     9901 atccgtataa cgttgactct gataatgcgt tgtatgtcct attgtaaaat ccttgaacat
     9961 agcgtaattt gataaggaag ttgtgtataa aggattgtac ggaatccgtc gtacattatc
    10021 gggatcgctg gtatcaacgg ccttcgcacg tgaaagttca agtttggttt cccacatttg
    10081 ttctttttcg tacttcatcg ctatttccca accccgtgat gaacttttgc gaatattttc
    10141 cggatgaatt attccggcca aattccacgc gatctgattg tgcgctctca ttacaaacca
    10201 agtatattcg atttcaaagc tatgctttgc ttgagaacca ccgaagcgcc cgcccaaatc
    10261 ccatgttgtt gatcgttcag gtttgatatc cggattgcct gatgcgtccg tatctttcca
    10321 atatcgttca ttgagcgtag gcgctctaaa attacgagac catgatgcgc gtaaggacgt
    10381 gtaatagcct gatttttcgt aggcaatatt cattccggcc ttgggactcc atacctgccc
    10441 aaaatctgaa aatttatccc atcgtatagc cggtgataat cgtaaagacc
//
LOCUS       DAOQGF010000108        10479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_2963_length_10479_cov_6.360994, whole genome shotgun sequence.
ACCESSION   DAOQGF010000108 DAOQGF010000000
VERSION     DAOQGF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_10479_cov_6.360994"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..3108
                     /locus_tag="PK937_12725"
     CDS             4..3108
                     /locus_tag="PK937_12725"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNH33482.1"
                     /translation="MLNGVNYEGAKGLRFNRFFTKENIHPFDEIVWEKRSSVITEPDG
                     RVVFEMRDLEIPREWSQLATDIAASKYFRKAGVPGTGREISVKQLVHRVAHTIRIEGE
                     AMGYFDSSADAEVFELELTHLLVNQKGAFNSPVWFNCGLYHEYQIKGSGGNFYWNAQS
                     GKIEMTEDSYSKPQCSACFIQSVSDDLMGIFELAKNEAKLFKYGSGTGSNFSSLRGKQ
                     EKLSGGGTSSGLMSFLTVLDRGAGATKSGGTTRRAAKMVCLDMDHPEIVDFINWKVKE
                     EKKVKALIDAGYESDFNGEAYQTVSGQNSNNSVRISDKFMNAVLENGKWDTIARTTGK
                     TVETHDARNLYEQIAYAAWACADPGVQFDDTINDWHTCPNTDKIHASNPCSEYMFLDD
                     SACNLASINLMKFADESGKFDIEGFRQSCRIFIIAQEILVDMSSYPTQQIAQNSHDYR
                     PLGLGYANLGTLLMVNGLAYDSEEGRAMAGAITAIMHAWGFRGSAEVASIKGPFAGYK
                     KNEKPMLRVMNKHRDAAYKINPEFSHDEALLKAAQEDWDTCVKMGEKYGYRNAQATVL
                     APTGTIGLLMDCDTTGVEPEFSLVKWKKLAGGGYFKIVNNSIPQALRNLGYSADDIDG
                     IIKYILGHGTFEGAPYVNPEQLKQMGFADAEIAEAEAYVQKNKSLDEWTPHINIKSLK
                     TKGLSGEQISRAVIYIGGAQTVEGTHLLKEEHLAVFDCANKCGIGKRFIDPMGHIKMM
                     SAAQPFLSGAISKTVNIPNEASVKDIEKIYFEGWKLGLKAVALYRDGSKFSQPLSSKG
                     ISTESEAGQSAEEVKAQIDAAVKAAIEHAAKELRRGQKKPMPLKRNGFTQEVELGGHK
                     IYFRTGEYEDGQIGEVFIDMFKEGAAYRSLLNCFAVAVSMGLQYGVPLEKFVEKFTFT
                     RFEPSGFTNHPNIKNATSIIDFIFRVLAMEYLGRMDFVHVPPQIDTATAPVAQSGTNE
                     TPTASHSHTHDTHAHDASANPMEKQLSEMMGDAPACDVCGHMTVRNGACYKCLNCGNS
                     MGCS"
     gene            3428..4345
                     /locus_tag="PK937_12730"
     CDS             3428..4345
                     /locus_tag="PK937_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNH33483.1"
                     /translation="MDSTSVNAWNSHASAIPTLYALLVVRRSGLVFEAYYQSTDSTTF
                     FAQRSVTKSIIGALTGVALYDHTIHNVSDKLSRYFPKYYEDISDNRARQITLKHLLTM
                     SAGYAWNEVADRLYGNYVSFAVHLPMDTVPGRVFNYSSAVPHIISGILSDRTDNKLIS
                     WSRNKFFSKLGIDSMHWQADPQGIPLGGTGLYLRARDMAKIGMTYLDGGCYDGQRVFS
                     DLWPDISFTDALDDNHAYGHLWWLDEAMDYPAAYAFGYGGQLIYVVPALDIVVIATAD
                     PNVNYTVSDQVLTSVRQIIHNDILPSVIP"
     gene            4358..5701
                     /locus_tag="PK937_12735"
     CDS             4358..5701
                     /locus_tag="PK937_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNH33484.1"
                     /translation="MPNSKTKPETKLFILDTNVILYDYTCIYNFKEHDVVIPIVVLEE
                     IDQFKKGSDLINFQAREFIREMDKLSKAQLFNKGLQLKKGHGRLFVEVGEAHSELVYN
                     AFTTNKPDHRILALADQLRHKRTDREVIIISKDINLRMKAKSLGIPAEDYETGKIRNV
                     DELYTGIQTHENFNTEVIRRFYEQTEAVPADDLKLPRKPVANEYYILKNHSSSVLTHY
                     NPSAQGLERIEKKRAYGIEPRNAEQVFALDALMRPEVAVVTLTGKAGTGKTLLALAAA
                     LQQRQLYSQIYLARPVVPLSNRDIGYLPGDVHEKLDPYMQPLWDNLAVIKNKFSQEST
                     EVTRIAEMLEREKLVIAPLAYIRGRSLDKIFFIVDEAQNLTPHEIKTIITRAGEGAKM
                     VFVGDIYQIDSPYLDTQSNGLAYLVDRMKGQDLFAHVNLLKGERSALAELASNLL"
     gene            5795..6763
                     /locus_tag="PK937_12740"
     CDS             5795..6763
                     /locus_tag="PK937_12740"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNH33485.1"
                     /translation="MLTKLARTIKDYNDSGFSSTINNQGKRLMNADGRFNVIKRGASF
                     ADRFSYLNALLTMSWGRFFAVIFGIYTAINLVFASVYFVIGREHFSGLLSSDTASEYI
                     EDFFFSAQTLTTVGYGRVNPTGVAANIVASAEALIGLLCFALATGLLYARFSRPDAKI
                     VWSDKVLIAPYRDGQAVMMRLANSKDTQLMDVEVNMMFSMVLDESGTPTRKFFNLNLE
                     RSTVNFLPLNWTLVHPLNTESPLYGLTPADLMQGEAELIVSLKGTEETSMQIASARRS
                     YLHADFEWNARFLLMFAPSPDGTATVLHLDKVGIFERLHNVKETAA"
     gene            complement(6765..7043)
                     /locus_tag="PK937_12745"
     CDS             complement(6765..7043)
                     /locus_tag="PK937_12745"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNH33486.1"
                     /translation="MKWLGWLHKPKSAISVEIFSKSDCHLCDEMSDAFHGLQNEFPMT
                     IKYTDITSDEALFEKYRYDIPVVFVNGRKAFKHRATTDAIRKRLRREI"
     gene            complement(7040..9022)
                     /locus_tag="PK937_12750"
     CDS             complement(7040..9022)
                     /locus_tag="PK937_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNH33487.1"
                     /translation="MAPLRRMILTLFYPLAFILASNNGGNPLVDSLSSISPESPYYST
                     AMVRLSEILMTENTVLSRRAAEEAMRNAIRTSPDDPWHYVRLSKIQFERSFYRSAFAA
                     CDDAIRINQSKPVPYHDVFSEAYYYKGLIKERQALKYKDMISPVNEDFSISLSNFGYE
                     DMLESARYYEKALEYNGTHRDALFHLGLLYYEIRAFDKMSALFEKIIQYKPEDKDALM
                     FLGLAYYHRGDFGQSHTYYQRAIHAMSPEEASRVNHIGHIVPRDDKENYLSRAGSPTQ
                     SSAYAAQFWRKRDPLLSSDYNERMLEHYNRMAYVNLRFGIPRQNIMGWETERGMIYIR
                     YGKPSQWVRTQPDEKMLGGTEIWYYPRFSFRFEDHYSSGKFLLDNPSLLASESAYNEH
                     SDDYVFPAERLFPVSARVYQFKSQSGLNKIRVYYSALIKSMDPDYDTYGPTGKTQIGF
                     FITDSERNILSKTMDTSLVEPKETRDAYVYKAATMETFIQSKEPVKWSLELKSLGEFR
                     YAMAGDSLTLRSFSSRTLDMSDVVLADYVDENKPYAVFANTQGVLQADKPLYFFAEVY
                     NLTLEKDQSRFVVEAGLAPHATGGIRTFLQKIFGNTDALVYSTFEQTGTSRDDTVTFA
                     LNVSEVPPGNYDAVVRIRDLISGHTTEQKTSIRIVR"
     gene            complement(9123..9602)
                     /locus_tag="PK937_12755"
     CDS             complement(9123..9602)
                     /locus_tag="PK937_12755"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNH33488.1"
                     /translation="MITDFFRRRLIQPLMDLLRQGLSAEKLALSVSLGFVLGIFPMIG
                     ATTILCLVIGALFRVNQLAIQTVNYFLFPVQIAFYIPFFQMGAWLFGYDHFPFTFEEI
                     ATMLSRNPWQAIVSLWWANLRAITAWLLTAPLLGGILYLILVPVFKPITARFFPDKA"
     gene            complement(9599..>10479)
                     /locus_tag="PK937_12760"
     CDS             complement(9599..>10479)
                     /locus_tag="PK937_12760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNH33489.1"
                     /translation="LYHRLRENTVRLARMNIQAHYDLSNEFFKTFLDSSMTYSSAYFR
                     TPDMSLEAAQTEKYDRLCRQLKLKPTDHVLEIGSGWGGFALHAVKHYGCKITTITISE
                     EQYHYARARFAAEGFADRISILFTDYRNVSGQFDKIVSIEMLEAVGHRYFKTYFAKLH
                     EVLKPNGLVGLQVIICPDSRYAALRKGVDWIQKHIFPGSLLPSVGVLHEAAHRTGDLS
                     LVDLKHLGRHYARTLATWRDRFNQNNELVQALGFDESFRRKWNYYLSYCEAAFESRNI
                     GVVQMIWSRPNNMLVS"
BASE COUNT         2843 a         2362 c         2580 g         2694 t
ORIGIN      
        1 gcgatgctca atggcgttaa ttatgaaggc gcgaagggtc ttcgatttaa tcgctttttt
       61 accaaagaaa acatacatcc atttgatgag atcgtgtggg aaaagcgttc cagcgtgatc
      121 accgaacccg atggtcgcgt cgtatttgaa atgcgtgacc tggagatacc ccgcgagtgg
      181 tctcagttgg ctacggatat cgcggcttcc aaatatttcc gtaaagccgg tgtgccggga
      241 acgggacgcg aaatcagcgt caaacaactt gttcatcgtg tggcccatac gattcgcatc
      301 gaaggcgaag cgatggggta ttttgattcg tcggccgatg cggaagtttt tgaattggaa
      361 cttacgcacc tgcttgtgaa tcagaaaggc gccttcaatt caccggtatg gtttaactgc
      421 ggtctttatc atgaatatca gattaagggc agcggtggta atttttactg gaatgcgcaa
      481 tccggcaaaa tcgaaatgac ggaagattcg tattccaaac cccaatgctc ggcgtgtttc
      541 attcaatccg tgtcggacga tctgatgggt atttttgaat tagccaaaaa cgaggctaaa
      601 cttttcaaat acggttccgg tacgggttct aatttttctt cgttgcgcgg taagcaggaa
      661 aaactttccg gcggcggtac atcgtccggt ttgatgtcct ttctgactgt tctggatcgc
      721 ggtgccggtg ctaccaagtc cggcggcacg acgcgccgtg cggcaaagat ggtctgtctt
      781 gatatggatc atcctgaaat cgtagatttt atcaactgga aagtgaaaga agagaaaaaa
      841 gtcaaagcgt taattgacgc cggatacgaa tccgatttca acggcgaagc gtatcagacg
      901 gtgtccggac agaactccaa taattccgta cgtatcagcg ataaatttat gaatgccgtt
      961 ttggaaaacg gcaaatggga tacgatcgca cgcaccacgg gtaaaacggt ggaaacccac
     1021 gacgcgcgca acttgtatga gcaaatcgct tatgcggcct gggcatgcgc cgacccgggc
     1081 gtacagtttg atgatacgat caatgattgg catacgtgtc ccaatacgga taagatccat
     1141 gcatccaatc cctgctcgga atacatgttc ctcgatgact ccgcctgcaa cctcgcttcc
     1201 atcaacctga tgaagtttgc cgatgaatcg ggtaagtttg acatcgaagg ttttcgtcaa
     1261 tcgtgccgca tttttatcat tgcgcaggaa atccttgtgg atatgtccag ttatccgacg
     1321 cagcagatcg cgcaaaacag tcatgactac cgcccgctcg gtttgggtta tgccaacctt
     1381 ggtacgctcc tgatggtcaa cgggcttgca tacgactccg aagaaggccg cgcgatggcc
     1441 ggtgcgatca cggcgatcat gcacgcatgg ggattccgcg gttctgcgga agtggcttcc
     1501 atcaaaggac cttttgcggg ttataagaaa aatgaaaaac ccatgttgcg tgtaatgaat
     1561 aagcaccgtg acgccgcata taaaatcaac ccggaattca gtcacgacga agccttgctg
     1621 aaggcggccc aagaagattg ggatacctgc gtcaagatgg gcgaaaaata cggctaccgt
     1681 aacgcccaag ctaccgtgtt ggctccgacc ggtacgatcg gattgctgat ggattgcgat
     1741 acgacaggtg tggaacccga attttcgctc gtcaaatgga aaaaattggc cggcggcggt
     1801 tatttcaaaa tcgtcaacaa ttcgatcccc caagcattgc gcaatctcgg ttactccgcc
     1861 gatgatattg acggtatcat caaatatatt cttggtcacg gtacgttcga aggtgcgccg
     1921 tacgtcaatc ccgaacaact gaaacaaatg ggttttgccg atgcggagat cgccgaagcc
     1981 gaagcttacg tacagaaaaa caaatcgctt gatgagtgga cgccgcatat caatatcaaa
     2041 agcctcaaaa ccaaaggcct cagcggcgaa cagatctccc gtgcggtgat ctatatcggc
     2101 ggtgcgcaaa cggtcgaagg cacgcattta ctcaaagagg aacatttggc cgtatttgat
     2161 tgtgccaata aatgcggtat cggtaaacgg tttattgacc cgatgggaca tatcaagatg
     2221 atgtccgccg cgcaaccttt cctgtccgga gcaatttcca agacggtgaa tattcctaac
     2281 gaagccagcg tcaaagatat cgagaaaatc tacttcgaag gctggaagct cgggctgaaa
     2341 gccgtagcgc tgtatcgcga cggatccaag ttttcccaac cgctgagcag caaaggcatt
     2401 tcgacggagt ctgaggccgg acaaagtgcg gaagaagtga aagcccaaat tgatgctgcg
     2461 gtcaaagctg cgatcgaaca tgcggccaaa gaattgcgcc ggggccagaa aaaaccgatg
     2521 ccgctcaaac gtaacgggtt tacccaagag gttgagcttg gcggtcacaa gatttatttc
     2581 cgtacgggtg aatacgaaga cggtcagatc ggtgaagtat ttatcgatat gtttaaagaa
     2641 ggagcggctt atcgcagttt gttaaactgt tttgccgtgg ccgtctccat gggtttgcag
     2701 tacggtgtgc cgcttgaaaa atttgtagag aagtttacgt tcacacggtt tgaaccgagc
     2761 ggttttacaa accatcccaa tattaaaaat gcgacatcca tcatagactt cattttccgt
     2821 gtattggcga tggagtacct cggacgtatg gacttcgtac atgtgccgcc gcagattgat
     2881 acggctacgg caccggttgc ccaatcgggg acaaacgaaa cgccgacggc ttcacattcc
     2941 catacgcatg atacccatgc gcatgatgcc tcagccaatc cgatggaaaa acaactgagc
     3001 gaaatgatgg gtgatgcgcc ggcttgcgat gtatgcgggc atatgacggt tcgtaacgga
     3061 gcttgctaca agtgtctgaa ctgcggtaac agtatgggct gctcgtaatc cgggcgtatg
     3121 gttttgaaaa aaattgcagc gttttttggg aagtgaccga ctataaagaa aaggtcgaat
     3181 ctactgaatg ctgactgtcc ttgagttatg aatagagagc acgatgatga tccggtaacg
     3241 gatgtattgt cgtgcttttt ttataatagg attattcagt gatacggatg tttctataca
     3301 aataatgatc caaaaaaaaa tcattctaac ggtattactt tttacctcct gtcgtagcaa
     3361 tgacctgcag gaaccgccgt gcgatgaaac ggccgctttg cgccgtatat cttttgcgga
     3421 agcgagaatg gattctacaa gcgtcaatgc gtggaacagt catgcatccg cgatcccaac
     3481 actatacgcc ttattagttg tgcggagaag cggtttggtt tttgaagcct actatcagtc
     3541 caccgattca acaacgtttt ttgcacaacg ttcggttacc aaaagcatca tcggcgcttt
     3601 gaccggagtt gcgctgtatg atcataccat ccacaatgtt tccgataaac tcagccggta
     3661 tttcccaaaa tattatgaag atatcagtga taaccgtgcg cggcaaatca cattgaagca
     3721 cctcctgacg atgtctgcgg gatatgcgtg gaatgaagtc gccgatcgtt tgtatgggaa
     3781 ttatgtttca ttcgcagtac acctcccgat ggataccgtg ccggggcgtg tttttaacta
     3841 cagttcggcc gtgccgcata ttatatcggg tatattgtca gatcgcaccg ataataaatt
     3901 aatatcatgg tctaggaata aatttttttc gaagcttgga atagattcga tgcattggca
     3961 ggccgatccg caggggattc ctttgggcgg tacgggttta tatctgcggg cacgggatat
     4021 ggccaagatc ggtatgacgt atctggatgg tggttgttac gacgggcagc gtgttttttc
     4081 agatttatgg ccggatattt cttttaccga tgctttggat gataaccatg cgtatggcca
     4141 tctgtggtgg ttggatgagg ctatggatta tccggcggct tatgccttcg gttatggcgg
     4201 acagctgatc tacgtcgtac cggcgctgga tatcgtggtg atcgcaaccg ccgaccctaa
     4261 tgtcaactat accgtatccg atcaagtcct gacatccgtt cgccaaataa ttcacaacga
     4321 tattcttcct tccgtgatcc cttaaccaag gaagcctatg ccgaattcta aaaccaagcc
     4381 cgaaacgaaa ctttttatcc tcgacaccaa tgtgatcctg tatgactaca cgtgcattta
     4441 taattttaaa gagcatgatg tcgtgatacc catcgtcgta ctggaagaaa ttgatcaatt
     4501 caaaaaaggc agtgatttga tcaattttca ggcgcgcgaa tttatccgcg agatggataa
     4561 gttatccaag gcacaattat tcaataaagg ccttcaatta aaaaaaggcc acgggcgttt
     4621 atttgtcgaa gttggtgagg cgcattcgga gctcgtgtat aatgccttta cgaccaataa
     4681 acccgatcac cgaattttgg cgctggcgga tcagctaagg cataagcgta cggatcgcga
     4741 agtcatcatc atcagcaaag acatcaatct ccggatgaaa gccaaatcgc tcggcattcc
     4801 ggcggaggac tatgagaccg ggaaaattcg caacgtggac gaactgtaca cgggcattca
     4861 gacgcatgaa aattttaata ccgaagtgat ccgcagattt tatgagcaga ccgaggccgt
     4921 cccggccgat gatctcaaac tgccgcgtaa accggtagct aatgaatact atattctcaa
     4981 aaatcacagc agcagcgtac tcacacacta taatccatcg gcgcagggtt tggaacgtat
     5041 cgagaaaaaa cgagcgtatg gcatcgaacc ccgcaatgcg gagcaggtgt ttgcattgga
     5101 tgcgctcatg cgcccggagg ttgcggtggt tacgctgacg ggtaaagccg gtacgggcaa
     5161 aacgctgctg gcactggccg ccgcactgca gcaaagacag ctttacagtc aaatatactt
     5221 agcacgaccg gtcgtaccgc tcagtaatcg ggatatcgga tacctgccgg gcgatgtcca
     5281 tgaaaaactt gatccgtata tgcaaccgct gtgggataat ctggccgtga taaaaaataa
     5341 attttcacag gaaagcaccg aagtgacacg cattgctgaa atgcttgagc gcgaaaaatt
     5401 ggtcattgcg ccgctggctt atatccgcgg gcggagcctg gataaaatat tttttatcgt
     5461 agatgaagcg caaaacctga cgccgcatga aataaaaacc attatcaccc gtgcgggcga
     5521 aggtgctaaa atggtatttg tcggcgatat ttatcaaatt gattcaccgt atcttgatac
     5581 acagtccaat ggtttggcgt atctggtaga tcgcatgaaa ggtcaggatc tgtttgcgca
     5641 tgtcaatttg ctcaaagggg agcgcagcgc cttggcagaa ttggccagta atctgttata
     5701 atggttcgtc ggtaaccgat tagcgaccgt cattgcagtc aaaaatttat aaaaaaatac
     5761 tttttaattc acctacgaat tggcagaaaa acctatgctc acgaaacttg cgcgcacgat
     5821 caaagattac aacgattccg gttttagcag cacgatcaat aatcagggca aacgtctgat
     5881 gaatgccgac gggcgattta atgtgatcaa acgtggcgca tcctttgcgg atcgttttag
     5941 ttatctcaat gcgttactaa ctatgagttg ggggcgattt tttgccgtta tctttggtat
     6001 ctatactgcg attaaccttg tgtttgcatc ggtatatttt gtgatcggcc gggaacattt
     6061 ttcaggctta ttgtcctctg atacagccag tgaatatatc gaagattttt tctttagcgc
     6121 acagactctt acgacagtgg gttacgggcg cgtcaatccc acaggcgttg cggccaatat
     6181 tgtcgcttcg gcggaggcat tgatcgggct actctgtttt gctcttgcga ccggcttact
     6241 ttatgctcgt ttttccagac cggatgccaa aatcgtttgg agcgataaag ttttgatcgc
     6301 tccgtatcgc gacggacagg cggtcatgat gcggctggcc aattccaaag acacacagct
     6361 gatggatgtc gaagtcaata tgatgttttc aatggtattg gatgaatcgg gaacgccgac
     6421 gcgtaaattt tttaatctga atttggagcg ttccacggtc aactttcttc cgctcaactg
     6481 gactttagta catccgttga acactgaaag tcctttgtat ggtttgacgc ctgccgattt
     6541 gatgcaaggt gaggccgaac tcatcgtatc gctcaaaggc acggaagaaa cttccatgca
     6601 aattgcgagt gcacggcgtt cttatttgca tgccgacttc gaatggaatg cgcgtttttt
     6661 gctgatgttt gcgccatcac cggatggaac ggcaacggtg ctccatttgg ataaagtggg
     6721 gatttttgaa cgcttgcata acgtaaaaga gaccgcggct tagtttatat ctctctgcgc
     6781 aaacgttttc gtatcgcatc ggttgttgca cgatgcttga atgctttgcg accattgaca
     6841 aatactacgg gaatatcgta gcggtatttt tcaaacaagg cttcatcgga agtaatatcg
     6901 gtgtatttta tggtcatggg gaattcattt tgcagaccat gaaatgcatc ggacatctca
     6961 tcgcataaat gacaatccga tttgctaaaa atttcaacgg aaatagctga tttgggttta
     7021 tgaagccatc ctaaccattt catcgtacga tcctgatgga tgttttctgt tcggtggtat
     7081 gtccggaaat caaatccctt atacgcacta cggcatcata gtttcccggc ggtacctcac
     7141 tgacattgag cgcaaaggta acggtatcat cacgggacgt gccggtttgt tcaaacgtcg
     7201 aatacaccaa ggcatcggtg tttccgaaaa ttttttgcaa aaaggttcgt atgccacccg
     7261 tcgcatgcgg cgccaatccg gcttccacga caaaacggct ttgatctttt tctaaagtaa
     7321 ggttgtagac ttcggcaaaa aagtataggg gtttgtctgc ctgcaaaacg ccttgggtat
     7381 tggcaaaaac ggcataaggt ttattttcgt caacataatc ggccaaaaca acatcgctca
     7441 tatccaaagt gcgtgacgaa aaacttcgga gggtcaacga atcgccggcc atcgcataac
     7501 gaaattcacc caaagatttt aattcgagag accacttcac gggttcttta ctttgtatga
     7561 aggtctccat cgtggccgct ttgtatacat acgcatcccg ggtttctttt ggctcgacga
     7621 gcgaagtatc catggttttt gataaaatgt ttcgctcgga atctgtgata aaaaaaccga
     7681 tttgtgtttt gccggtcgga ccatacgtgt cgtaatcggg atccatggac ttgatcaggg
     7741 cagaataata cacgcgtatt ttgttaagcc cggattggga tttgaattgg tacactctgg
     7801 ctgacacagg gaataaacgt tcggccggaa atacgtaatc gtccgaatgc tcgttatagg
     7861 ccgattccga tgcgagcagt gagggattat ccagcaaaaa ttttccgctg ctgtaatgat
     7921 cctcaaaacg aaatgagaac ctcggataat accatatttc ggtaccgccc agcatttttt
     7981 cgtccggttg cgtgcgcacc cattgcgagg gtttgccgta ccgaatgtag atcatacccc
     8041 gttctgtttc ccaacccatg atattctgtc gcggtattcc aaaacgaaga ttgacgtacg
     8101 ccatacggtt ataatgctcc aacatacgct cattgtagtc cgacgacaac aggggatcgc
     8161 gcttccgcca aaactgtgcg gcataagctg atgattgcgt ggggcttccg gcgcgggaca
     8221 aatagttttc tttatcgtct cggggtacga tatgtccgat atgattaaca cgcgaagctt
     8281 cttccggcga catcgcatgg atggcgcgtt ggtagtaggt gtggctttgt ccaaaatcgc
     8341 cgcggtgata ataagccagc cccaaaaaca tcaaggcatc tttatcctcc ggcttatatt
     8401 gaataatttt ttcaaacaaa gccgacattt tatcaaacgc acggatttca taatataaca
     8461 accccagatg aaaaagggca tcccgatgtg tgccattata ttccagcgct ttttcgtagt
     8521 atcgcgctga ttccaacata tcttcgtaac cgaaattgga taaagaaatg ctgaaatctt
     8581 cgttaacggg gctgatcata tctttgtatt tgagcgcttg ccgttctttg atcaggcctt
     8641 tgtagtaata ggcttcggaa aaaacatcat gataaggcac tggtttagat tgattgatcc
     8701 ggatcgcatc atcacaggcg gcaaacgcag aacggtaaaa actgcgttca aactgtattt
     8761 tgcttaaacg tacatagtgc cacggatcgt cgggtgatgt gcgtatagca ttgcgcatag
     8821 cctcttccgc ggcacgtcgg gatagcacag tgttttccgt catgagaatt tcagacaggc
     8881 gtaccatagc cgtggaatag tatggtgatt cgggcgaaat gctcgaaaga gaatctacaa
     8941 gtggattccc gccgttatta ctcgccagta taaaagcaag cggatagaac aacgtaagga
     9001 tcatcctacg cagcggtgcc atgcgtctat agctttcaaa aaaaggatta tgatactaca
     9061 acgaaaaacg taatcaagtt attccggcta tccgatgaag atacattgct gagttttgga
     9121 attcaagcct tgtccggaaa gaaacgcgct gtgataggtt tgaaaacggg aactaaaatc
     9181 agatagagta ttccgcctaa aagcggcgcc gtaagaagcc atgccgttat agcacgcaga
     9241 ttggcccacc atagggatac gatggcttgc catggattac ggctgagcat ggttgcgatc
     9301 tcttcaaacg tgaaaggaaa atgatcgtat ccgaaaagcc aagcacccat ctgaaaaaaa
     9361 ggaatataaa aagcgatttg tacaggaaag agaaaataat taaccgtttg aatggccagt
     9421 tggttaacac gaaaaagcgc tccgatcacc aaacataaaa tggtcgtggc gccgatcata
     9481 gggaatatac ccaacacaaa accgaggctg acactcaacg caagtttttc ggcggaaagg
     9541 ccttggcgta acaaatccat cagcggctga ataaggcgtc gtcgaaagaa atcggtgatc
     9601 atgaaaccaa catattattg gggcgtgacc atatcatttg tacaacacct atattacgcg
     9661 attcgaaagc cgcttcgcaa tagctaagat aataattcca tttccgtcgg aaactttcat
     9721 caaaacccag cgcctgtacc agttcgttat tctgattaaa gcgatcacgc caagtggcca
     9781 atgtgcgggc ataatgacga ccaagatgtt tgagatcaac cagtgaaaga tcgccggtgc
     9841 gatgagcggc ctcatggagc acacctacgg atggcaacaa ggatccggga aaaatatgtt
     9901 tttgaatcca atccacacct ttgcgcaacg cggcgtagcg cgaatcgggg catataatga
     9961 cttgcaagcc gacaaggcca ttgggtttta gcacttcatg aagttttgca aagtaagttt
    10021 tgaaataacg atgccctacg gcctccagca tttcgatgga tacgattttg tcaaattggc
    10081 cgctgacatt gcgatagtcc gtaaacaata ttgaaatccg atccgcaaat ccctcggccg
    10141 caaaacgtgc gcgggcataa tgatactgct cttctgaaat cgtaatcgtc gtgattttac
    10201 aaccgtaatg tttgaccgca tgcaaggcaa acccgcccca gccgctaccg atttcgagga
    10261 cgtgatccgt cggcttgagt ttcagttgac ggcaaaggcg gtcgtatttt tccgtttgcg
    10321 cggcttccag cgacatatcg ggcgtacgga aatacgcgct tgaataagtc atggaagaat
    10381 ccagaaatgt tttaaaaaat tcattgctca gatcgtaatg agcctgaata ttcatacgcg
    10441 caagcctgac ggtattttct cgtaaccgat gatataaac
//
LOCUS       DAOQGF010000109        10323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3019_length_10323_cov_6.971075, whole genome shotgun sequence.
ACCESSION   DAOQGF010000109 DAOQGF010000000
VERSION     DAOQGF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3019_length_10323_cov_6.971075"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PK937_12765"
     CDS             <1..1486
                     /locus_tag="PK937_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNH33490.1"
                     /translation="DYRPDIYGKIGNSGVSICTLDDMKKLYAGFDLCDPNTSVSMTIN
                     GPAPMILAMFFNTAIDQQIEKHLRQNGKTDKLNKTKFDHSHPHYEKAIQYDGVLFGYG
                     TLGTTGDRLVDRETYEKIKADTLKVVRGTVQADILKEDQAQNTCIFSTEFALKMMGDM
                     QDYYVRKQIRNHYSVSISGYHIAEAGANPITQAAFTLSNGFTFVEYYLSRGMHVDDFA
                     PNLSFFFSNGLDPEYSVIGRCARRIWSIAMKYKYKGNDRSQKLKYHIQTSGRSLHAME
                     IDFNDIRTTLQALMAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAK
                     NENSLQGSFIIEELTDLVEEAILGEFDRISERGGVLGAMETMYQRNKIQEESLLYESL
                     KHDGKLPIIGVNTFLNPKGVSEAQPTELIRSTKEEKEQQIVNVHNFWKTNDGTAQGAL
                     DMLSQVALQNGNIFEQLMETAKSCSLGQISSTLYKVGGQYRRNM"
     gene            1506..2660
                     /locus_tag="PK937_12770"
     CDS             1506..2660
                     /locus_tag="PK937_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH33491.1"
                     /translation="MLISKIILKNWRNFKKVEVDLHDRSFIVGPNASGKSNLLDAIRF
                     LRDIAKSGGGLQEAIRTRGGVSKLRCLAARSQAEIEVEAHIAENGIVLWKYAIGFSQT
                     PGGISEPKAKLKYEKVWNHNDDLIINRPDSEDEKDERLLEYTLLEQPTGNRKFRDVGD
                     FFSSIQYLHIVPQLLKDPKSYIHSTQEDYFGRDFMDRINKTNKNTRVSFLNKIEKALQ
                     IALPQFNKLELIKDHEGIPHLQARYKHWRARDAKQWEDQFSDGTLRLIGFLWSLLEGT
                     KPILLEEPELSLHSGIVSKLAEVIHKLQQKKKGKRQVILTTHSYDLLSNPGISAEETI
                     LLEPSDEGTSAKNAASNVEIKTLLTEGLSIAEIVIPKTSPKNINQIVLEF"
     gene            2663..3277
                     /locus_tag="PK937_12775"
     CDS             2663..3277
                     /locus_tag="PK937_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33492.1"
                     /translation="MNKPVIRLVVEDELHQNVLIRILKHFDDKFEIGDVYGFRGNSYI
                     KKKLHAFNEASQLSPYLVLTDLDNYACPPTLIGEWVNFTISHNMIFRIAVKEAESWLL
                     SDRKNFASYFRISAATIDLNIEAIDNPKEYLVNLARKSKNRKIREDFVPNGTALVGPN
                     YNARLADFIHNHWDYNNARRNNRSLHKLMNRLDNFVFKPAHKLK"
     gene            3281..3610
                     /locus_tag="PK937_12780"
     CDS             3281..3610
                     /locus_tag="PK937_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNH33493.1"
                     /translation="MDTIILLTISNIFMTFAWYGHLKYKDSPLWIAILISWGIAFVEY
                     CFQVPANRWGHGRFNAAELKTIQEAITLTVFCVFSVLYLKEELKWNYLVGFALMIAAV
                     FFIFKKW"
     gene            3661..5103
                     /gene="xdhA"
                     /locus_tag="PK937_12785"
     CDS             3661..5103
                     /gene="xdhA"
                     /locus_tag="PK937_12785"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HNH33494.1"
                     /translation="MKNTISFLLDGRHESITFGPDCPYTPITTVLQYLRSLPDRKGTK
                     EGCAEGDCGACTVVIGETAQAGTVRYTAVDSCLIFLPMLHGKQLLTVESLTRDEKHLH
                     PVQHALVEKHGTQCGFCTPGFVMSMFALYKNHPNPTRETINDALTGNLCRCTGYRPIV
                     EAAATVCVNERHDALSENESRHYDQLSTIIHGTIAIQTAHHRYVKPATLSEALLYLAE
                     HPEALIISGATDIALRVTKKHESLTSILDLSDVPELKDVSESGDKLILGAGLSLNQVR
                     AIVKTSHPALYDILQVFGSLQIRHLATLGGNLGSASPIGDTLPVLLAYQAKILLESVH
                     GQRRVDMDAFITGYRTTVRKADEIITAVKLPKIQPGTIVRSYKVSKRKDLDISTVSAA
                     FRLERSNDGAIRKIIMAYGGMAEKTKRAERTEAFLTGKPWTRATIESAMPLIDLEFRP
                     ISDARSGAEFRRTVARNLLLKFWSETNLMR"
     gene            5390..5719
                     /locus_tag="PK937_12790"
     CDS             5390..5719
                     /locus_tag="PK937_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HNH33495.1"
                     /translation="MRIENAPFQIFDLFQTSPVAHQGETGQALWQTVSNGNVRMRIVT
                     YSPGYLADHWCEKGHAVFVLEGAFVSELQDGRRFELTQGMSYIVADHAEPHRSYSASG
                     VKLLIVD"
     gene            5756..8971
                     /locus_tag="PK937_12795"
     CDS             5756..8971
                     /locus_tag="PK937_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNH33496.1"
                     /translation="MKHSFPSVLFFLGLFIAYVPCTHAQNETFEMVIQSSHTGEVASV
                     DFAPDGKTFITASHDRTVRLWSRDGRLMRIFTAEEPLWSARFSPDGKHIIAAGSGNLI
                     HVWNVLGKLENIFETDQGPITDIQFAPDGQRFAFGNHDDVVLGNLDGTFEKALEGHRG
                     LVLGVAFSPDGLLIASCSEDKTVRIWNCFGDSVGIIRNFSNKVSHVIFSPDGTSIFTS
                     GFDPVIREWSLEGKLLRAFYGHRDNVYAVTISPDSRFVISGGYDRALRLWSRDGKLLR
                     TFETMHREAISDVRFSPDGKDIISASFDKSAIIWKIDGTWVRTLGNTYKPGEGGHWVQ
                     SAVFTPDGQHMIAAHWDERIVVWNTIGGIRCRVRTPSKIISSLDVAPLGNFFAVGTGD
                     GYVHIYNLDGFLQMTFNAQSPVDCIAFTSDGERLLSGGFDQRISVWNLKTKMRERTFG
                     PTKDRVNSIAVSPDGKSVVTGSNFGEVKLWDMDGTARKTFSGHGDYGMIVSTRYASNG
                     RYFASLSVNDQRSLIVRDLSGATVLDIPTAHNKNFGAVAFSPDGRYIACTVDDKVQLF
                     RFDGTLSKTFAGHTGYVNTLTFSSDSKHLLSGGDDGSIRLWHVGSLESAVYVSDDEEW
                     VMFTPEGFFDASLGGGKFTAMVQGMNAFSMDQFASVNNRPDLILERLDIGDETLRRHY
                     EAQFLKRVRMAQEPPAFTAAPSATIDSCRIEEDKAILTTTLVSSSAALRSYQIYVNNV
                     PLFDAPGGQLSGYHTTTCDTIHLTPGLNKIEISCVDAMNVESLRAWQQVSCTKPSASE
                     LYFLGFGVSHYRDTMLSLQYADKDVKDLAGTFEKMNAGPFARVHTRLFIDDSASTASL
                     SLATNFLNKAKENDVVILFIAGHGLHDESPDATYYFLPYEADRDALTTTAIPFDAFEQ
                     LLHRTKARRKLFFMDTCESGEADEGRGAISSLNTTKTIRSRAARGFKSSSGQRSYLFQ
                     KDRYIYNNLLRRSGTIVFSSSLGGEYSYERDDFQNGLFTEALIAALGGAADANYDAKV
                     DTYELRRYLRKTVSSWSNGMQNPTVDRDNISQLISLPVLRR"
     gene            9016..>10323
                     /locus_tag="PK937_12800"
     CDS             9016..>10323
                     /locus_tag="PK937_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH33497.1"
                     /translation="MHSSIPHDSAAKHVSGRSVYIDDIPEPPQMLHGHVVYSAHAHAR
                     ITHIELEDARASAGIHAVLTWRDIPGHNQMGPVVHDEPCLAEHETTCIGQAIVLIAGD
                     SPEACFQAAKKIKIEYEPLEVITDLPTAIQKNALLGPARKIRRGKPDEYLQNATHRLN
                     GSLFTGAQEHWYLETQTCLAVPGEGDEINLYSSTQHPSETQAIVSEVLGIAKHDVVVE
                     VRRMGGGFGGKETQGNHTAAWAALLARSTGRSVKIRLFRDDDQIMTGKRHPFLSHYEV
                     GFDDHGKITALKIELNADAGAATDLSFAILERAMLHSDNAYFIPHIEIIGNAWKTNLP
                     SNTAFRGFGGPQGMAVIENIVDQIARSLQKDAAEVRQINFYGTNENQTTPYGQVIEQN
                     RLPVIFEQLMNTSDYWERRRAIHLFNAENEFQKKGMALTPVKFG"
BASE COUNT         2881 a         2449 c         2343 g         2650 t
ORIGIN      
        1 cgattaccgc cccgacattt acggcaaaat cggaaattcc ggcgtttcga tctgtacgct
       61 cgacgatatg aaaaaactgt atgccggatt tgatctctgc gatcccaata cgtccgtatc
      121 catgacgatc aacggccccg cgccgatgat tctcgccatg ttctttaata ccgccattga
      181 tcaacagatc gaaaaacatc tacggcaaaa cggtaaaacg gataaactta acaagaccaa
      241 atttgatcac agccatccgc attatgaaaa agccatccaa tacgacggtg tgcttttcgg
      301 atacggtacg ctcggcacga cgggagaccg cttggtagat cgagaaacct atgaaaaaat
      361 caaagccgat acgctcaagg tcgtgcgcgg tacggtgcag gcggatattc tcaaagaaga
      421 tcaggcacag aacacatgta tcttttcgac ggagtttgcc ctcaaaatga tgggtgatat
      481 gcaggattat tacgtacgta aacaaattcg caatcactac tccgtcagca tttccggata
      541 ccacatcgcc gaagccggcg ctaacccgat cacgcaagcc gcttttacat tgtccaacgg
      601 attcactttt gtggaatact atctctcgcg cggaatgcat gtggacgact ttgcgccgaa
      661 tctttcgttc tttttcagca acgggcttga tccggaatat tccgttatcg gtcgttgtgc
      721 acggcgtatc tggtctatcg ccatgaagta caaatacaaa ggcaatgacc gttcgcaaaa
      781 actcaaatac cacatccaga ccagcggtcg cagtttgcat gcgatggaga ttgatttcaa
      841 cgacattcgc accacgttgc aagcactgat ggcgatctac gataactgca attcgctcca
      901 caccaatgcc tacgacgaag cgatcacgac gccgacggaa gaatccgtac gacgtgccat
      961 ggcgattcag ctcatcatca ataaagaact cggtttagcg aagaatgaaa attcactcca
     1021 gggctctttc atcatcgaag agttgaccga tctcgtggaa gaggcgattt tgggcgaatt
     1081 tgaccgcatc tccgaacgcg gcggtgtatt gggcgcgatg gaaacgatgt atcagcgcaa
     1141 taaaatacag gaagaatctt tgctgtacga aagcctcaaa cacgacggca aacttccgat
     1201 catcggcgtc aatacgtttc tcaatcccaa aggcgtaagc gaagcgcaac cgacggaact
     1261 catccgctcg accaaagaag aaaaagaaca gcaaatcgtc aacgtgcata atttttggaa
     1321 aaccaacgat ggcacggctc aaggcgcctt ggacatgctg tcccaagtgg ctctgcaaaa
     1381 cggcaatatt ttcgaacaac tgatggaaac cgccaaatcc tgctccttgg gacaaatcag
     1441 ttccacgctg tataaggtcg gcggtcaata ccgcaggaat atgtaaaaca aacaacaaga
     1501 aaaacatgct gatttccaaa attattttaa aaaactggcg caactttaag aaagttgaag
     1561 tcgatctaca tgatagatcg tttattgtag gacccaatgc atccggaaaa tcgaatttat
     1621 tggatgcgat ccgattttta cgggacatcg ctaaaagcgg aggagggctt caagaagcaa
     1681 tacgaaccag aggcggcgtt tcaaaattac gatgccttgc tgctagatct caagctgaaa
     1741 ttgaagttga agcccacatt gcagaaaatg gaatagtttt atggaaatat gcgattggtt
     1801 ttagtcaaac tccgggcggc atatcagaac caaaagctaa attaaaatat gaaaaagttt
     1861 ggaatcacaa tgatgatctg ataataaaca gacccgatag cgaagatgaa aaagatgaaa
     1921 gacttttaga atatacgctt ttggaacaac caacaggcaa tcgtaaattt agagatgtcg
     1981 gagatttttt ttcttctatt caatacttac atattgtgcc tcaactacta aaagatccaa
     2041 aatcttacat acactcaacg caagaggatt acttcggtcg tgattttatg gatagaataa
     2101 ataaaacgaa taagaataca cgtgtatcat ttctaaataa aattgaaaag gctcttcaaa
     2161 ttgcattgcc tcaatttaac aaattagaat taataaagga tcacgaaggt atccctcatc
     2221 ttcaagcgag atacaaacat tggcgagccc gagatgcaaa gcaatgggaa gatcaatttt
     2281 cagacggcac attacgttta attggttttt tatggtcatt gttagaaggt acaaagccaa
     2341 tactacttga agaacccgaa ctgtcgttac attcgggtat agtttctaag ttagcagaag
     2401 ttattcataa gcttcaacag aagaaaaaag ggaaaaggca ggtaatttta acgactcaca
     2461 gttatgactt gttatccaat cccggaatta gcgctgaaga aaccatttta ctagaaccgt
     2521 ctgatgaagg tacttccgcc aaaaacgcag ccagcaatgt tgaaatcaaa actctattaa
     2581 cagaagggtt gagcattgcg gaaattgtaa ttcctaaaac ttcaccgaaa aatattaatc
     2641 agattgtatt agagttttga cgatgaacaa accagttatt cgtttggttg ttgaggatga
     2701 attacatcaa aatgttttaa ttagaatctt aaaacatttt gatgacaaat ttgaaattgg
     2761 cgatgtttat ggcttcagag ggaacagtta tattaaaaag aaattacatg ctttcaatga
     2821 agcatcacaa ttaagtcctt atttagtcct gactgatcta gacaactatg cgtgcccacc
     2881 gaccctaata ggtgaatggg ttaattttac aatatcgcac aacatgattt ttagaatcgc
     2941 agtaaaagaa gccgaaagtt ggttattaag cgatcggaaa aattttgcaa gctattttag
     3001 aatatctgcc gctacgattg atttgaatat agaagccata gacaatccca aggagtattt
     3061 ggttaactta gctcgaaaat caaaaaacag aaaaataaga gaggattttg ttccgaacgg
     3121 aaccgcacta gtcggaccta attacaatgc gcgacttgct gacttcatcc ataatcattg
     3181 ggattacaat aacgctagaa gaaacaaccg gagtcttcat aaactaatga accgcttaga
     3241 taactttgtt tttaagccgg ctcacaaatt aaaataatgc atggacacaa tcatactcct
     3301 gacgatttcc aatatcttta tgacttttgc atggtacggt catctgaagt ataaagattc
     3361 gccgctgtgg attgccattc tcataagttg gggtatcgcg tttgtggaat attgttttca
     3421 agtgccggcc aatcgctggg ggcacggacg atttaatgcc gcagaactca aaaccattca
     3481 ggaagccatc acgctcaccg tattttgtgt gttttccgta ttgtacctca aagaagaatt
     3541 aaaatggaat tacctcgtgg gtttcgctct gatgatcgcc gctgtttttt tcatattcaa
     3601 aaagtggtag tcccgtcacg cgttatcccc tatcacagct gatatcctga aagttggctc
     3661 atgaaaaaca cgatttcctt tttactcgac ggtcggcacg aatctattac gttcggaccg
     3721 gactgtcctt atacaccgat cacgacggta ctgcagtacc tgcgcagttt gccggatcgc
     3781 aaaggcacca aagaaggttg cgcggaaggc gactgcggcg cgtgtacggt ggtcatcggc
     3841 gaaaccgcac aagccggcac ggtacgctac accgccgtgg acagttgttt gatattttta
     3901 ccgatgctcc atggtaaaca gcttctgacc gtagaaagcc tcacacgcga tgaaaaacat
     3961 cttcaccccg tgcaacatgc cttggtcgaa aaacacggaa cgcaatgcgg tttttgtacg
     4021 ccgggtttcg tcatgtccat gttcgcactg tataaaaatc atcccaaccc cacgcgcgaa
     4081 accatcaacg atgcgctgac cggtaatctc tgccgctgca cgggttaccg tccgatcgtc
     4141 gaagcggcgg ccacagtctg cgtgaatgaa aggcacgacg cgctgagcga aaacgaatcg
     4201 cgccactacg atcagctttc gacgatcata cacgggacga ttgcgattca gacagcgcac
     4261 catcgctatg tcaaacccgc cacactcagt gaagccttac tttaccttgc tgaacacccg
     4321 gaagccttga tcatcagcgg cgccaccgac atcgcactgc gtgtgaccaa aaagcacgaa
     4381 tcgcttactt ctattctcga cctctccgat gtaccggaac tcaaagatgt atcggaatcg
     4441 ggcgacaaac tgatcctcgg tgccggactt tcgctcaacc aagtgcgcgc aatcgtaaaa
     4501 acttcacacc ccgcgctgta tgacatactc caagttttcg gttcgctgca aatacgtcat
     4561 ctcgccacgc tcggcggaaa tctcggaagc gcttcgccca tcggcgatac gttgcccgta
     4621 ttattggcgt atcaagccaa aattttatta gaaagcgtac acggtcaacg ccgcgtggat
     4681 atggatgcat ttatcaccgg ttaccgtacg accgttcgta aagccgatga aatcatcacg
     4741 gctgtaaaac ttcccaaaat ccaacccggt acgatcgtac gttcatacaa agtatcgaaa
     4801 cgcaaagacc ttgacatatc caccgtcagc gcggcatttc ggcttgagcg aagcaacgac
     4861 ggcgccatcc gaaaaatcat tatggcttat ggcggcatgg ctgaaaaaac caaacgtgcc
     4921 gaacgcaccg aagctttcct caccggcaaa ccctggacac gcgctaccat cgaatccgcg
     4981 atgccgctca ttgatttgga atttcggccc atttccgatg cacgttccgg cgctgaattt
     5041 agacgcacgg tggcgcgcaa tctccttttg aaattttgga gtgagacgaa tttaatgcgt
     5101 taacagcctc gtaattgtgt acttgcgacg tacggttgat tcggcgacta tctttgagag
     5161 gtatgtgcac ttactatatc aaaaaatttc tttccatatc tctttgcggc tttgcgaggg
     5221 taaattcatt ctcgccaaga cgcgaagact caaaaattat cagaaccttc ataattgaat
     5281 ttaaatgcgc taaaacgacg agcgcttgat ttactgctta tattttgaca atcatgaggc
     5341 cgctattaat tcgttttttc actactctat cggttaagga gttttttcta tgcgtattga
     5401 aaacgcacca tttcagattt ttgatttatt ccaaacatcg ccggtggcgc atcaaggaga
     5461 aaccggtcaa gcgctgtggc aaactgtttc gaacggtaat gtacgcatgc gtatcgtcac
     5521 gtattcaccc ggttatctcg ccgatcattg gtgtgaaaaa ggccatgccg tatttgtgtt
     5581 ggaaggcgcc tttgtatccg aactgcaaga cgggcgtcga tttgaactca cgcaaggcat
     5641 gagttatatc gtggccgatc atgccgaacc gcatcgctcg tattccgcat cgggcgtcaa
     5701 acttctcatt gttgattaaa ttatactcta ctaataatta aaatttggag atcatttgaa
     5761 gcacagcttt ccgagtgtct tatttttttt gggattattc atcgcgtacg ttccctgtac
     5821 gcatgctcag aatgaaactt ttgaaatggt tatacaaagt tcgcataccg gtgaagtcgc
     5881 gtccgtggat tttgctccgg acggcaaaac cttcatcacc gcctcgcatg atcgaacggt
     5941 acgcctatgg agtcgcgacg ggcggctgat gcgtatcttt acggctgaag aaccgttgtg
     6001 gtcagcgcgc ttttcgcccg acggaaaaca tatcatcgcg gcaggtagcg gtaacctgat
     6061 ccatgtgtgg aatgtactcg gaaaattaga aaatatattc gaaacggatc aaggacctat
     6121 cacggatatt caatttgccc ccgacggtca acgattcgcc tttggaaatc atgacgacgt
     6181 tgttttggga aatcttgacg gcacgtttga aaaagctttg gaaggccatc gcgggttggt
     6241 acttggtgtt gccttttcac ccgacgggct tttgatcgcc agttgcagtg aggataaaac
     6301 cgttcgtata tggaattgtt ttggcgacag cgtcggtatt attcgtaatt tttcaaacaa
     6361 agtctctcat gtgatatttt ctccggacgg cacatctatt tttacctctg gtttcgatcc
     6421 ggtaattcgc gaatggtcat tggaaggcaa gctcctccgg gcattttacg ggcatcgcga
     6481 taatgtgtat gccgtaacca tatcaccgga cagtcgtttt gtgatttccg gcggttatga
     6541 tcgtgcactc cgattatgga gtcgtgacgg aaaacttctt cgtacgtttg aaacaatgca
     6601 tcgggaagct atttccgatg ttcggttttc acccgacggc aaagacatca tcagcgccag
     6661 ttttgataaa tcagccatta tctggaaaat agacggaaca tgggttcgta ctttaggaaa
     6721 tacctataaa cccggcgaag gcggccattg ggttcaatcg gcggttttca cgccggacgg
     6781 acaacacatg attgctgcac attgggatga acggatcgtc gtgtggaaca ccatcggcgg
     6841 gattcgctgc cgtgtccgca ctccgtcaaa aataatttcc tctctcgatg tagcgcccct
     6901 cggcaatttt ttcgccgtgg gtacgggcga tgggtatgta cacatatata atttggacgg
     6961 gtttttgcaa atgaccttta atgcgcaatc tcccgttgat tgtatcgcgt ttacctcgga
     7021 cggcgaacgc cttttatcgg gcggttttga tcaacgcata tccgtgtgga atcttaagac
     7081 aaaaatgcgc gagcgtacat tcgggcccac caaagatcgt gtcaattcga tcgctgtatc
     7141 gccggacgga aaatccgttg tgacgggcag taattttgga gaagtaaagc tttgggacat
     7201 ggacggtaca gcacgtaaaa cattcagcgg ccatggcgat tacggcatga tcgtaagtac
     7261 gcggtacgcc tccaatggcc gttactttgc ttcgctcagt gttaacgatc aaagaagtct
     7321 tatcgtacgc gatctaagcg gcgctacagt acttgacatt cctacagcac ataataaaaa
     7381 tttcggcgcc gtggcatttt ctcccgacgg acgttacata gcctgtactg tagatgataa
     7441 agtacaactt tttcggtttg acggtacact atccaaaaca ttcgcagggc atacgggtta
     7501 cgtcaatact cttacgtttt catccgacag caaacacttg cttagcggcg gtgatgatgg
     7561 cagcatacgg ctttggcatg taggcagcct tgaatcggca gtctatgtat ccgatgatga
     7621 agaatgggtt atgtttacgc ccgagggttt ttttgatgca tcgctcggcg ggggtaaatt
     7681 taccgctatg gtgcaaggaa tgaacgcctt tagcatggat cagtttgctt ccgtcaataa
     7741 ccggcccgat cttattctgg aacgattgga tatcggtgac gaaacgttac gccgccatta
     7801 tgaggctcag tttttgaaac gtgtgcggat ggctcaagaa cctcctgcct ttacagctgc
     7861 acctagcgct acaatcgaca gttgccgaat cgaggaggac aaggctattc tgaccactac
     7921 ccttgtatca tcctctgccg cattgcgttc gtatcaaatt tatgtaaata atgtcccttt
     7981 gtttgacgca ccaggtggtc agttatcagg ctatcacacg acgacctgtg acactattca
     8041 tttgacgccg ggattgaata aaatcgaaat atcctgcgta gatgcgatga atgttgaatc
     8101 cctgcgcgcc tggcagcaag tcagttgcac caaaccgtcg gcatccgaac tctattttct
     8161 tggctttggc gtttcacatt accgcgatac tatgctttca ctgcaatacg ccgataaaga
     8221 tgtcaaggac cttgctggta catttgaaaa aatgaatgca gggccttttg cacgtgtaca
     8281 tacacgactt tttatagacg acagtgcaag cacggcatcg ctttctctcg cgactaattt
     8341 tctgaataaa gccaaagaaa atgatgttgt gatccttttt attgcaggtc acggtctcca
     8401 tgatgaaagt cccgatgcca cctattattt tcttccgtat gaagccgacc gcgatgcgct
     8461 cactacgacc gctattcctt ttgatgcttt cgaacaattg ttgcatcgca ccaaagcccg
     8521 ccgcaaactg ttttttatgg acacctgcga atccggagaa gccgacgaag gccgtggcgc
     8581 tatatcttca ttaaatacta caaagaccat tcgatcacga gctgctcgcg ggtttaaatc
     8641 ttcttcgggc caacgctctt acctctttca aaaagaccgt tacatctata ataatctttt
     8701 acgtcgttct ggcacaattg tattttcctc atcactcggc ggggaatatt cgtatgaacg
     8761 cgatgatttt caaaacgggc tttttacaga ggctttaatc gccgcgcttg gcggagccgc
     8821 ggatgctaat tacgatgcga aggttgatac gtatgaactg cgccgttacc tgcggaaaac
     8881 ggtttcatcc tggtccaacg gcatgcagaa ccctaccgta gatcgggata atattagtca
     8941 actcatctcc cttccggttc ttcgccggta gtgcaacgcg aattgatttt accgaaaata
     9001 ttgatagatt taattatgca ctcctccatc cctcatgaca gcgcggccaa acatgtgtcc
     9061 ggccgttcgg tttatattga cgacatcccg gagccgccgc aaatgctgca cggtcacgtg
     9121 gtgtatagtg ctcatgccca cgcacgcatc acgcatatcg aacttgaaga tgcacgcgct
     9181 tccgcgggaa tccatgccgt gctgacctgg cgcgatatac ccggccacaa ccaaatgggg
     9241 cccgttgtac atgatgaacc gtgcctcgca gaacacgaaa cgacctgtat cggccaggct
     9301 atcgtactga tcgcgggtga ctcacccgaa gcatgctttc aggcggcgaa aaaaataaaa
     9361 atcgaatatg agcctctgga agtgattacc gatttgccga ccgcgattca aaaaaatgca
     9421 ctgctcggac cggcgcgaaa aatccgccgc ggcaaacccg atgagtactt gcaaaacgca
     9481 acgcatcggc ttaacgggtc gctgtttacc ggagcgcagg aacattggta tttagaaaca
     9541 cagacctgtc ttgcggtacc gggcgaaggc gatgaaatca atctctacag ttccacacaa
     9601 catccttccg aaacgcaagc gattgtctcc gaagttttag gcattgccaa acatgacgtc
     9661 gtcgtcgaag tgcggcgtat gggcggcggt ttcggcggta aagaaacgca aggcaaccac
     9721 acggccgcgt gggcggcatt gctggcccga tccaccggac gctctgtaaa aattcgacta
     9781 tttcgtgatg acgatcagat catgacggga aaacgtcacc cgtttttatc gcattatgaa
     9841 gtcggatttg atgaccacgg aaaaatcacg gctctaaaga tcgaactgaa tgccgacgcc
     9901 ggcgctgcta cggatttatc ttttgcgatt ttagaacgtg cgatgttgca tagtgacaat
     9961 gcttatttca tcccccatat cgagatcatc ggcaacgcat ggaaaaccaa tctgccatcc
    10021 aacacagcat ttcgcggatt cggcggccca caaggtatgg ccgttatcga aaacatcgtt
    10081 gaccagatag cgcgaagcct ccaaaaagat gccgcagaag tccgtcaaat taatttttac
    10141 ggaacgaacg aaaatcagac gacgccttac gggcaagtga tcgaacaaaa tcgtttgccg
    10201 gtcatctttg aacaacttat gaatacttcc gattattggg agcgccgccg cgctattcac
    10261 ctgtttaacg ccgagaatga atttcaaaaa aaagggatgg ccctgacgcc ggtaaaattc
    10321 ggt
//
LOCUS       DAOQGF010000110        10316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3022_length_10316_cov_5.760745, whole genome shotgun sequence.
ACCESSION   DAOQGF010000110 DAOQGF010000000
VERSION     DAOQGF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_10316_cov_5.760745"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            532..1620
                     /gene="had"
                     /locus_tag="PK937_12805"
     CDS             532..1620
                     /gene="had"
                     /locus_tag="PK937_12805"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HNH33498.1"
                     /translation="MSTQIDAWQMTTSGQPMNRVASAIPSLGPDEALIRIAACGVCHT
                     DLGFYYDGVRTVSPLPLTLGHEISGQVVDTGLSATAWKNKFVLIPAVLPCGTCEMCQA
                     GQPRICRHQKMPGNHIHGGFASHIVVPAHFLCPVEVGGADELFGKSGVTLRELCAIAD
                     AITTPFQSIKQTGLIKDDVAIFVGAGGVGGFGIQIAQSIGAHVVVIDVDDKKLEIYKN
                     FGAELTFNPKTVEPKEMRKSIQAWVQQNKYRSYGWKIYETSGTAKGQELAFGLLTFGS
                     VMGVIGFTLDAVQLRLSNLMAFDAKAIGNWGCEPGLYPEVLQWVKEGRIQIAPFVQKF
                     PMSEINSVFDRLHHRQIDRRPILIPDFS"
     gene            1633..2721
                     /gene="oah"
                     /locus_tag="PK937_12810"
     CDS             1633..2721
                     /gene="oah"
                     /locus_tag="PK937_12810"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HNH33499.1"
                     /translation="MFKNHDLVPDYEFKEIRYELKPCKDPSGHVLPDLFSAWITLNNP
                     SQLNSYTTTMVKEVILAFRKASNSRNVIAAVFTGEGDRAFCTGGNTKEYAEYYSGQPE
                     EYKQYMRLFNDMVTSILHCDKPVICRVNGMRVAGGQEIGMACDFSIASDLAVFGQAGP
                     KHGSAPDGGSTDFLPLFVGIENAMISGTLCQLWSAHKALRLGILTDIVPVLKKDGQFI
                     PNPLVITDRYTDNMGRIVHGEFHTGAEKDKAKEIMKSCVTDFSKLDATVDQLITSLLM
                     MFPGCVSKTIESLRKHKLAHWDKNRETNRSWLGLNMMTEAKAGFRAFNEGTKESREVD
                     FIKMRQLLAAGEKWSDELIEKIHPKNKK"
     gene            2740..3501
                     /locus_tag="PK937_12815"
     CDS             2740..3501
                     /locus_tag="PK937_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH33500.1"
                     /translation="MPNKIKEQITDGILHLTLCNTRGNILDGEMMGEITSAIEGATRD
                     PHIKAITFRGEGDHFSFGASVPEHQKDRVAEMLASFHKLFRTLISFSRPTIAIVRGQC
                     LGGGMELAAFCHWIFAHEKAQFGQPEIQLAVFPPVASLALPLRIGQPMSDDLNLTGRS
                     IGAEEAYRIGLVQHISPDPDTTASEFIRQHIIPKSASSLRIATTASRRIYHEQFLKHI
                     DALEAMYLQELMGTHDANEGINAFMQKRKPEWQNR"
     gene            3486..4208
                     /locus_tag="PK937_12820"
     CDS             3486..4208
                     /locus_tag="PK937_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH33501.1"
                     /translation="MAKPMIGIDLSNKVAVLTGAAGGIGSVIAQKLEKAGAKVYAVDM
                     IQPPHGLWKKTDVGSFDETGRCVEEILKEASRIDILINNAGITRDAVIWKMDESDWDD
                     VMRINLKGAFNLIHHISPAMRYQNAGRIINITSINGMRGKFGQSNYAASKAGLIALTK
                     SVARELARHQVTANAIAPGLIGTPMVEKMPPESLQQSVQEICLGHPGKPEDIAYAVLF
                     LSSEWARHITGETLRVDGGQYM"
     gene            4222..5379
                     /locus_tag="PK937_12825"
     CDS             4222..5379
                     /locus_tag="PK937_12825"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNH33502.1"
                     /translation="MIHVPLEGKTLEETLYNCRELAEDPSFPTVKAWRENGGKVLGHF
                     QVYFPEELAYAAGMLPFKVCGAQVEGMEAESHFGSYLCSVIKTSLELALTKQITLDMF
                     VTHPICDAARNLAAIWGRNFSYPCQILYLPQNPNSRHSAEYLRGEYHRLLTDIETIVG
                     HKVRDEDIANAIGVYNESRRLMRELYDIKRETPWLIAAEDAYALIAIAGCLPRDAYNA
                     LMQRVLPQIRERRLQPKDKMRIVFEGGFCEQPPLDLIQVIGQSCYIVDDDLLIGLRYI
                     LEDVPSTGDPLANLAESYIERSSYSPVQHDLRKPKEEMLLERIRHSRAEAAIITAAKM
                     CEPGLEEQVAYTKALDQAGVPYFVSEFEENQTTFDHIGIQLETFVENLIFQ"
     gene            5399..6679
                     /locus_tag="PK937_12830"
     CDS             5399..6679
                     /locus_tag="PK937_12830"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNH33503.1"
                     /translation="MDTLVGRGTQRGAALFREWFDELGHAAETGKPSAYVFVMGSLNE
                     ILKSFDMPVVFPEINSLQTAVRRVAPEFLAEAEDYGYSPDICGYVKADVTVQLRKGQH
                     PMGRIPQPSVAVLTNACNTYIKWAEIWERMYKIPVVTIDVPGTRGRREDPFAKGSHFE
                     NDRKYIAAQLKELITTCEQITGKKFDIDKLRETMQYANDMSVSWKNILDCNKSKPSVL
                     NALTDGTIYLGVANGFRGSKEGSVYFKELEEEMKYKTANGIGTVTDEKFRLAFVGVPC
                     YPIFRRFNDFFSDWGGTFVQSTYLYFAAGGANLGFQYDLQNPIDSLAEGVLFSVRDAM
                     DSMFHQDQVLLSMIDDYAIEGVVYHPIKSCRTVSTGLADGRKHLMAQSDVATLFLESD
                     MMDKRVVSEAQMKNRVDAFFEGLISRRQIAAAAV"
     gene            6703..7515
                     /locus_tag="PK937_12835"
     CDS             6703..7515
                     /locus_tag="PK937_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNH33504.1"
                     /translation="MAYSAGVDVGSTQTKAVIINEQRQIVQRALIDTGANVTLAAQKA
                     FRQAAEAAQVSEEEIEYVIGTGYGRYKVTFGNKQITEISCHGRGAVHMFPKTRTVLDM
                     GGQDSKAIRVSDKGEIIDFCMNDKCAAGTGRFLGAAAMTLHIPLDNLGQTALEAMKAV
                     RITTTCTVFAESEILSWLGKGKKVEDILWGVHQSIAARSIGLLRRVGIEDEITFTGGV
                     ARNVGMIKALEDSLQRKINVGADSHFMGALGAALFAMDHIVAARQPQKEAVA"
     gene            7512..8330
                     /locus_tag="PK937_12840"
     CDS             7512..8330
                     /locus_tag="PK937_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNH33505.1"
                     /translation="MNCTLGLDVGSTYTKAIILDEDKKIRGKYMAATGSRLNEVAEEV
                     ITQACKDGGILRENIRYTMTTGYGRHLVDTRDMQVTDLTAAARGAQFLYPKTRTILDI
                     GGQTMKASRVDDRSKVLTFRLNDKCAAGTGAFIEKTARYMGYSTTDIGSLLNLSRSEV
                     GISGVCAVFAESEVISHLSEGVPPEDIMFGAILSLTDRSVQLLKRIKAESEFTLIGGI
                     LRWEKMVNSIREKLTGTVNVPEGDMPQFTAALGCALLARIRQSQTRERSASVTA"
     gene            8327..8929
                     /locus_tag="PK937_12845"
     CDS             8327..8929
                     /locus_tag="PK937_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNH33506.1"
                     /translation="MSTRNIAILTEEHRIIEYVLNALERMANKTATEHTVDVAIGRKV
                     ITFLREFADQCHHGKEEAILFKWLNARHIGMDAVAMLEDEHVAGRDLVVRMQRALDEF
                     EKNKEIGSFADDANTFIAMLRDHIHKEDHGVFAMADGHIEAADDVRLIGDYLITERDG
                     RRHQKALALAQDIGHLMNMPGFDISIIPELSKTFINSNHA"
     gene            8922..9191
                     /locus_tag="PK937_12850"
     CDS             8922..9191
                     /locus_tag="PK937_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33507.1"
                     /translation="MPDFKPLHVIQTSSDGGCINADETRLIGQGWQRRYVADPRAAQE
                     AKLNYEEMGFEVQLLPIDVQSVSAECNGCRAAIEKYQIIYTRKSS"
     gene            9188..>10316
                     /locus_tag="PK937_12855"
     CDS             9188..>10316
                     /locus_tag="PK937_12855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNH33508.1"
                     /translation="MNEPQAVLIDAIRTPIGKIGGTLSSVRPDDMMGNLLNAMSVRHP
                     WLTHQCDDVIVGCANQAGEDGRNIARMAILSSNFPFEIPGQTINRLCSSSMQAVASAA
                     MTIQSGFARTLLAGGVESMSRAPYAVAKAEKAFPNGPMTMYDTALGWRFINPRITKRV
                     RPLSMGETAEEIFKQFAITRETQDAFAVASHRKAIAARNKGSWKDEIIALRVRQGKNE
                     VLIDTDECPREDASIETLSKLKPAFRDDGTVTAGNSCPMNDGASLLLLVSPDVAKESG
                     HREGFVLRGYAVTGIHPDIMGLGPVEATRRALQYAHVSLNDIDLIEINEAFAIQVLAC
                     MQALELDDTRVNVNGGAIALGHPLGCSGARIIGSLTNEMKRR"
BASE COUNT         2734 a         2549 c         2532 g         2501 t
ORIGIN      
        1 acatcggttg tctttccggt gcgtgcccgc tgcaatccag cttgtgggtc aatctattac
       61 ttggcgcttt gatcggttat acgatcgccg acaccctacg tacactgccc gaaaaatcct
      121 agtttgaccc atccgttata tttaaaatat attttctttt ttaaagagca gttcaaaaaa
      181 actgttcttt tttttggtta atccgaaaat aaataacaga cattaacatg accttactca
      241 tgttttagat aaaacttgag gtttgttatc tatttacatt tatgagattt gaatgctttt
      301 ttgaaacatg aaagtcaccg tatgcatagc aacctcctgt atggcataac accaatctat
      361 tgactacagt cattttgaga taaccatacc tcccctatct ttccagccgc tacgtttatc
      421 ttaacatttg ttctgttttc tttttcattt ctgaacagcc gattcggctt atttttttac
      481 accggtattc gaacgaacgt tcgaaaaatc tcttacacga ggcattggat tatgtcaaca
      541 caaatagacg catggcaaat gacgacttcg ggacaaccga tgaaccgcgt cgcatcggcc
      601 ataccttctt taggtccgga cgaagcactg attcggattg ccgcatgcgg cgtatgtcat
      661 acggacttgg gcttttatta cgacggcgtc cgcacagtca gtcccttgcc tttgacgctc
      721 ggccatgaaa tcagcgggca ggtcgtggat accggtttat ccgcgacagc atggaaaaac
      781 aaattcgttc ttattccggc cgtacttccg tgcggaactt gcgaaatgtg ccaagccggt
      841 caaccacgca tctgccgtca tcaaaaaatg ccgggtaacc acatccacgg cggttttgct
      901 tcgcacatcg tcgtaccggc gcacttttta tgtccggtcg aagtcggcgg agcggatgaa
      961 ctctttggga aatccggagt caccttgcgt gaattgtgcg ccatcgcaga tgcgatcacg
     1021 acgccgtttc aatccatcaa acaaacgggt ttgatcaaag atgatgtggc tatttttgtg
     1081 ggtgcgggcg gcgtgggtgg atttggtatt cagatcgccc aatctatagg tgcacatgtt
     1141 gtcgtcattg acgtggacga caaaaaatta gaaatctata aaaatttcgg cgcggaactg
     1201 acatttaatc caaaaaccgt cgaacctaaa gaaatgcgca aaagtattca ggcgtgggtt
     1261 caacaaaaca aataccgctc ctatggatgg aaaatctacg aaacgtcagg tacagccaaa
     1321 ggccaggaac ttgcgttcgg cctactcacc ttcggctccg tgatgggggt aatcggattt
     1381 acgctggatg cggtccaact ccgactcagc aatctgatgg cgtttgatgc caaagctatc
     1441 ggtaactggg gttgcgaacc gggtctgtat cccgaagtac tgcaatgggt caaagaggga
     1501 cgcattcaga tcgcaccctt cgtgcaaaaa tttccaatga gtgaaatcaa ttctgtattt
     1561 gatcgcttgc atcatcgtca gattgacagg cggccgatac ttatacccga ctttagttaa
     1621 tcaggagatt ccatgttcaa aaatcatgac cttgtcccgg attatgagtt caaagaaatc
     1681 cgatatgaat tgaaaccttg caaagatcct tcggggcatg ttttgccgga tcttttcagt
     1741 gcatggatta cactcaataa tccgtcacag cttaattctt acacgaccac gatggtcaaa
     1801 gaagtgatcc tggcctttcg caaagcttcc aattcccgca atgtcatcgc tgccgtattt
     1861 acgggtgaag gcgatcgcgc attttgtacg ggcggcaata ccaaagaata cgccgagtat
     1921 tattccggtc aacccgaaga gtacaaacaa tatatgcgtt tgtttaatga catggtgaca
     1981 tccatccttc actgtgacaa accggtgatc tgccgtgtta acggtatgcg agttgccgga
     2041 ggtcaggaaa tcggcatggc ctgtgatttt tccatcgcca gcgatcttgc cgtattcggt
     2101 caggcgggtc ccaaacacgg ctccgcaccg gacggcggtt ccaccgattt tcttccgctt
     2161 ttcgtcggca tagaaaacgc catgatctcc ggaaccttgt gtcaactatg gtctgctcat
     2221 aaagccctgc gccttggcat tctaaccgac atcgttcctg ttttgaaaaa ggatggacaa
     2281 tttataccca atcctcttgt gatcacagat cgttacactg acaacatggg acgtatcgtc
     2341 catggtgaat ttcacaccgg cgcagaaaaa gataaagcca aagagatcat gaaatcctgc
     2401 gtaaccgatt tttcaaaact ggatgcgacg gttgaccaac tgatcacgtc actgctcatg
     2461 atgttcccgg gttgcgtaag taagacgatc gagtcgttgc gtaaacacaa actggcgcat
     2521 tgggataaaa accgtgagac caaccgttca tggctgggcc tcaatatgat gaccgaagcc
     2581 aaagcgggtt tccgcgcatt caacgaaggt accaaagaaa gccgcgaggt ggacttcatc
     2641 aaaatgcgtc agcttttggc cgccggagaa aaatggtccg acgagttgat cgaaaaaatt
     2701 catcccaaaa acaaaaaata aactatacaa cgacaatcca tgcctaataa aataaaagaa
     2761 caaatcactg acggcattct ccatctgacg ctttgcaata cgcgcggaaa cattttggac
     2821 ggggaaatga tgggtgaaat cacctcggcg atcgagggag caacccgcga tccacacatc
     2881 aaagccatca catttcgcgg ggaaggcgat catttttcat ttggcgccag tgtaccagag
     2941 catcagaaag atcgcgtcgc ggagatgctt gcttcgtttc ataaactttt ccgtaccttg
     3001 atttcttttt cacgcccaac gattgcgatc gtgcgcggcc aatgtctcgg cgggggaatg
     3061 gagttagccg cgttttgtca ttggatattt gctcatgaaa aagcgcaatt cggtcaaccg
     3121 gaaattcagt tagccgtatt tcctccggtc gcttcattgg ctttaccgct gcggatcggt
     3181 caaccgatgt ccgacgatct caatcttacc ggaagaagca tcggcgcaga agaagcgtat
     3241 cgcatcggac tagtacaaca tatttccccc gaccccgaca cgaccgcatc ggaatttatt
     3301 cgccagcaca ttatccccaa aagcgcttct tctttacgta tcgctacgac cgccagtcga
     3361 cgcatctatc acgaacaatt tttgaaacac attgatgctt tggaagcgat gtatttgcag
     3421 gaactgatgg gcacgcatga cgccaatgag ggaatcaacg cgttcatgca aaaacgaaaa
     3481 ccggaatggc aaaaccgatg atcggaattg atctatcaaa taaagtcgcc gtactcaccg
     3541 gagcggcggg gggtatcggt tcggtcattg cgcaaaagct tgagaaggcc ggcgcaaaag
     3601 tttatgccgt agatatgata cagcctccgc atggtctttg gaaaaaaacc gatgtcggaa
     3661 gttttgatga aaccggccga tgcgtcgaag agattctgaa agaggcaagt cgcattgaca
     3721 ttttgatcaa taacgccggc attacaaggg acgcggttat ctggaaaatg gacgaatcgg
     3781 attgggatga cgttatgcgc atcaacctca aaggggcgtt taacttgatt catcacatat
     3841 ctcctgcgat gcgttatcaa aacgcgggac gcattatcaa tatcacgtcc atcaacggca
     3901 tgcgcggaaa atttggccaa tctaattatg ccgcaagtaa agccggactg attgcgctta
     3961 ccaaaagcgt cgcgcgtgaa ttggcgcgac accaagtgac agccaacgcc atagcacccg
     4021 ggctcattgg cacaccgatg gtcgaaaaaa tgccgcctga atcgctccag caatccgtac
     4081 aggaaatttg cttaggtcat ccggggaaac ctgaagacat cgcctacgcc gtactttttt
     4141 tatcatccga atgggcacgt cacatcacgg gcgaaacgct gcgtgtggac ggaggccaat
     4201 acatgtaatc cggaggaaat tatgatccac gtcccccttg aaggaaaaac gctcgaagaa
     4261 acgctctata actgccgcga acttgcggaa gatccgtcat tcccgacggt caaagcatgg
     4321 cgggaaaacg gcggaaaagt actcggtcat tttcaggtat attttccgga agaacttgcg
     4381 tatgccgcag ggatgttgcc ttttaaagtt tgtggtgcgc aagtcgaagg catggaagcc
     4441 gaatcgcatt tcggttcgta cctttgctcc gttatcaaaa cttcgctcga actggcgctc
     4501 accaaacaga tcacgctgga catgtttgta acgcatccga tctgcgatgc cgcacgtaat
     4561 cttgcggcta tatgggggcg taatttttca tacccttgcc agattttata tctgccgcaa
     4621 aatcccaatt cacgccattc ggcggaatat ctgcgcggtg aatatcatcg cttactcacg
     4681 gatatcgaaa ctattgtcgg gcataaagtc cgtgatgaag atatcgccaa tgcgatcggg
     4741 gtttacaatg aaagccgtcg tctgatgcgc gaactgtacg acatcaaacg cgaaacgcct
     4801 tggcttatcg ccgcagaaga tgcgtacgcg ctgatcgcaa ttgccggttg cctccctcgc
     4861 gacgcttaca acgccttaat gcaacgcgtt ttaccgcaga ttcgcgaacg ccgtttacaa
     4921 cccaaagata aaatgcgcat cgtatttgaa ggcggatttt gcgaacagcc accgctggat
     4981 ctcattcagg tgatcggtca gtcttgttac atcgtggacg acgatctttt gatcggactt
     5041 cgttacatcc tggaagacgt cccctcgacg ggcgatcctt tggccaatct cgcggaatcg
     5101 tatatcgaac gatcatccta ctctccggta caacacgatc ttcgtaaacc caaagaagaa
     5161 atgctcttgg aacgcattcg ccactcccgc gcggaagcgg ccatcatcac ggcggctaaa
     5221 atgtgcgaac cgggtttaga agaacaagtg gcttatacca aagcactgga tcaggccggc
     5281 gtaccgtatt tcgtcagtga atttgaagag aatcaaacga cgtttgatca catcggcata
     5341 caactcgaga cctttgtaga aaatcttatt ttccagtaac ccatcggaag gaatggctat
     5401 ggatacatta gtcggtagag gcacgcaacg gggagccgct ctctttcgcg agtggtttga
     5461 cgaactcgga cacgcagccg agacgggaaa accttcggcc tatgttttcg tcatgggcag
     5521 cctcaatgaa attttaaaat cctttgatat gccggtggtt tttccggaaa tcaattcact
     5581 acaaaccgcc gttcgccgcg tcgcaccgga gtttttggcc gaagccgaag actacggtta
     5641 ttcacccgat atctgcggtt acgtcaaagc ggatgtcacc gtccaactgc gcaaaggcca
     5701 gcacccgatg gggcgaattc cccagccttc ggtagccgtt cttaccaacg cctgtaatac
     5761 ctatatcaaa tgggctgaaa tatgggaacg catgtacaaa ataccggtcg tcacgattga
     5821 tgtaccggga acgcgcggtc gtcgcgaaga ccctttcgct aaaggcagcc actttgaaaa
     5881 tgatcgcaaa tatatcgcgg cgcaactcaa agaattgatc acgacatgcg aacagatcac
     5941 cggaaaaaaa tttgacatcg ataagctgcg cgaaacgatg cagtatgcca atgacatgtc
     6001 ggtgtcatgg aaaaatattc tcgattgcaa taaatccaaa ccctcggtgc tcaacgcgct
     6061 cactgacgga acaatctatc tcggcgtagc caatgggttc cgcggcagca aagaaggcag
     6121 cgtgtatttc aaagaactcg aagaagaaat gaaatacaaa accgccaacg gcatcggcac
     6181 cgtaacggat gaaaaatttc gcttagcttt tgtcggtgtg ccgtgttatc cgattttccg
     6241 tcgttttaac gatttctttt ctgattgggg cggtacgttt gttcagtcaa cttatctgta
     6301 ttttgccgcc ggcggcgcca atctcggctt ccaatatgat ctgcaaaatc cgattgatag
     6361 tttggcggaa ggcgtgcttt tcagcgtacg cgatgcgatg gacagcatgt ttcatcagga
     6421 tcaggtgctg ctttcgatga ttgatgatta tgcgatcgag ggcgtggtgt accatccgat
     6481 caaaagctgc cgtaccgtgt ccaccggatt ggccgatggg cgcaaacatc tgatggctca
     6541 gtccgacgtg gccactttat ttttagaatc agatatgatg gacaaacgcg tcgtgtccga
     6601 agcccaaatg aaaaatcgtg tagatgcttt ttttgaagga ctcatttcac ggcgccagat
     6661 agcggcggcc gcggtatgac aaaataacca aggagaatcc ttatggctta ttctgcaggc
     6721 gtggacgtag gttccaccca aaccaaagcc gtgatcatca acgaacaacg ccagatcgtg
     6781 caacgcgcac tgatcgatac cggcgccaat gtaactttag cggcgcaaaa agcatttcga
     6841 caagcagccg aagccgctca ggtttccgaa gaagaaatcg aatacgtcat cggcaccgga
     6901 tacggtcgtt ataaagtgac attcggcaat aaacaaatca ccgaaatcag ttgtcatggg
     6961 cgcggtgcag tacacatgtt tcccaaaaca cgcacggtgc ttgacatggg cggccaagat
     7021 agcaaagcaa ttcgtgtgag cgataaaggc gagatcattg atttttgtat gaatgacaaa
     7081 tgcgccgccg gtacgggacg ttttctcggc gcggcagcaa tgacattgca cattccgctg
     7141 gacaacctcg gacaaacggc gctcgaagcg atgaaggccg ttcgtatcac aacgacgtgc
     7201 accgtttttg cggaatcgga aattttatct tggttgggta aaggcaaaaa agtcgaagat
     7261 atactctggg gtgtacatca atctatcgcg gcgcgatcca tcggcctttt gcgccgtgtc
     7321 ggtatcgaag atgaaatcac ttttaccggc ggtgtcgccc gcaatgtcgg tatgatcaaa
     7381 gcgcttgaag attcgctgca acgcaaaatc aatgtcggcg ccgattccca cttcatgggt
     7441 gcgctcggtg cggcactttt tgcgatggat cacatcgtcg ccgcccgtca accgcaaaag
     7501 gaggctgtcg catgaactgc acactcggac tcgacgtagg ctctacctac accaaagcta
     7561 tcatcctcga cgaggataaa aaaattcgcg gtaaatatat ggcggcaaca ggctcccggc
     7621 tcaatgaagt ggccgaggaa gtcatcactc aagcctgtaa ggatggcggt attctgaggg
     7681 aaaatattcg ctacacaatg actacgggat atggtcgtca tctcgtagat acgcgggata
     7741 tgcaagtcac cgatcttacg gccgctgcac gcggagcgca attcttatat ccgaaaacac
     7801 gtaccatact tgatatcggc gggcaaacga tgaaagccag tcgtgtggat gatcgctcca
     7861 aagtgctcac atttcgcctc aacgataaat gcgcggcagg tacgggcgca ttcatcgaaa
     7921 aaactgcgcg ctatatgggt tatagcacca cagatattgg atcattgctc aatttgtctc
     7981 gttccgaagt cggcatctcc ggtgtgtgcg ccgtttttgc tgaatcggaa gtgatcagcc
     8041 acttgtcgga aggcgttccc ccggaagaca ttatgttcgg cgccatcctt tcacttaccg
     8101 accgttccgt acaacttttg aagcgcatca aagccgaaag cgaatttacg ctcatcggcg
     8161 gcattttacg ttgggaaaaa atggtgaaca gtatccgcga aaagcttacc ggcacagtca
     8221 atgttccgga aggcgatatg ccgcagttta cggcggcact cggatgcgct ttgctagctc
     8281 gtattcgcca gagtcagaca cgggaacgtt cggcttcggt gacggcatga gtacacgcaa
     8341 tattgccatc ttaaccgaag agcaccgcat catcgaatac gtcttgaacg ccttagaacg
     8401 tatggccaat aaaactgcaa cagaacacac cgtagatgta gctatcggtc gcaaagtgat
     8461 cacgttttta cgcgagtttg cagatcaatg ccaccacggc aaagaggaag ctattttatt
     8521 caaatggcta aacgctcgcc atatcggcat ggatgctgtc gcaatgctcg aagatgaaca
     8581 cgtcgccggg cgtgatctcg tcgtacgcat gcaacgcgct ttggatgaat ttgagaaaaa
     8641 taaagagatt ggttccttcg ctgatgatgc taataccttc atcgcgatgt tgcgcgatca
     8701 tattcacaaa gaagatcacg gagtttttgc tatggcggat ggacacatcg aagcggcgga
     8761 tgatgtgcga ttgattgggg attatttgat cacggagcgc gacgggcgac gtcatcaaaa
     8821 agcgttagct ctggcgcagg atatcggcca tctgatgaat atgccgggtt ttgatatttc
     8881 cattattccc gaattatcca agacttttat taatagcaac catgcctgat ttcaaaccat
     8941 tgcacgtgat tcagaccagt tccgacggcg ggtgtatcaa cgcggatgaa acccgtctga
     9001 tcggccaagg ttggcaacgc cgctatgtcg ctgatccgcg tgcggctcag gaagctaaac
     9061 tcaattatga agaaatgggt tttgaagtgc agttattgcc gatcgatgta caatccgtaa
     9121 gcgccgaatg caacggttgc cgtgcggcca tcgaaaaata ccaaatcatt tatacccgca
     9181 aatcgtcatg aatgaacccc aagctgtact tattgatgcg atccgcacac ccatcggaaa
     9241 aatcggagga acattatcct ctgttcgtcc cgacgacatg atgggaaatt tgctcaatgc
     9301 tatgtccgtt cggcatccgt ggctgaccca ccaatgcgat gacgtgatcg tcggctgcgc
     9361 caatcaggct ggcgaagacg gacgcaatat cgcacgtatg gctattttaa gttcgaattt
     9421 cccttttgaa atacccgggc aaacgattaa ccgtttatgc agttcatcca tgcaggccgt
     9481 tgcttcggcc gcgatgacga tccaaagcgg atttgcccgc acgctactgg ccggcggcgt
     9541 tgagagcatg tcccgagcgc cctacgccgt cgccaaagcc gaaaaggctt tccccaacgg
     9601 gccgatgacc atgtacgaca ctgcgctggg ttggcgattt atcaatccgc gtattaccaa
     9661 acgtgtgcgc cctctgtcta tgggcgaaac ggccgaagaa attttcaaac aatttgccat
     9721 cacccgtgaa acgcaagatg cttttgccgt agccagtcat cgcaaagcta tcgctgcgcg
     9781 gaataaggga tcgtggaaag atgagatcat tgcgctacgt gtgcgccaag gaaaaaacga
     9841 agtcctcata gatacggatg aatgtccgcg cgaagacgct tctatagaaa cattatccaa
     9901 acttaaaccg gcatttcgtg atgacggcac tgtcacggca ggaaacagtt gtccgatgaa
     9961 cgacggcgcg tcactgcttc tgcttgtttc gcctgatgta gcaaaggaaa gcgggcatcg
    10021 tgaaggattt gtactgcgcg gatatgcggt cacgggaatt catcccgaca ttatgggatt
    10081 aggaccggtg gaagcaacac gtcgcgcttt gcaatatgct catgtatctc tcaacgatat
    10141 agatttgatc gaaatcaatg aagcctttgc gatccaagtt ttggcttgta tgcaggcttt
    10201 ggaattggat gatacccgcg tcaatgtcaa tggcggcgcg atcgctttgg ggcacccgtt
    10261 gggctgttcg ggcgcacgca tcatcggttc acttaccaac gagatgaaac gccgtc
//
LOCUS       DAOQGF010000111        10152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3080_length_10152_cov_7.189957, whole genome shotgun sequence.
ACCESSION   DAOQGF010000111 DAOQGF010000000
VERSION     DAOQGF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3080_length_10152_cov_7.189957"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..749)
                     /locus_tag="PK937_12860"
     CDS             complement(45..749)
                     /locus_tag="PK937_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNH33509.1"
                     /translation="MYNKGYLAAGIGVFFLYFALSPVHAQTPTKPSMWYLTDLEKEVV
                     RELNRVRRDPQGYVTHLKALKKLYDGNMVYYPGEATLMTNEGVDAVDECIRFLENAQP
                     IDTLGPSEGMSKAARDHMKEQSRSGKTGHTGDDGSSPFDRIERYGQWERTAGENIDYG
                     SNDARRIVISLLVDDGVPSRGHRENIFNPEFRKVGVACGTHKQYRYMCVMTLAGGFSA
                     KKNAANTRAENPADDK"
     gene            880..3129
                     /gene="pbpC"
                     /locus_tag="PK937_12865"
     CDS             880..3129
                     /gene="pbpC"
                     /locus_tag="PK937_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNH33510.1"
                     /translation="MKKNPDLIFIVLAVIGTFFYPISGDDLHFRDEASLRIYDRNGLL
                     LREVLSREGGRGQWVSPDQIDTRIIKAAIAAEDKRFFSHRGMDVLALGRAIGQNIMAM
                     SVVSGGSTISQQVIRNIYHFPRNPFYKLVELWYAIRLEYTLSKNEILAQYLNRIPFGN
                     QAFGVEAASRLYLGKSSQTVTWSEAAFLMALPKSPTRYNPYRNDTAAKNRRIAVLQRL
                     LVMQAIPNDDYERAVSEPILLHTQPHNFFAPHFTDYLLQHHAHLSTAWHTTLDLPLQQ
                     SAERILREHITYLQPENVTNAAAIILDNRTGALRVMAGSYDYFDAQHDGQFNAALARR
                     QPGSALKPFTYAIAFERNYTAASILADVQASFSTANGTFTPMNYDRRYHGPVRARIAL
                     ACSYNIPAVRVLQDVGVPVLLSKLHECGIQSLDQSPAYYGHGLTLGNGEVTLLELTRA
                     FSVLANQGALIQTQVFSDSVSRVERERIFTPESIFLVSDILSDPIARIPAFGYDSPLS
                     FPYPVAAKTGTSSDFRDNWTIGYTRDYTVGVWVGNFDNTPMDRISGITGAGKIFHALM
                     DALYPSTAPSGFVAPHTLHKIPICALSGQRAHPGCGAVIDEYFMKISSVQTTCTWHDQ
                     RGIIRYETIPQEYRAWFSSAGNHASVHASAPDNISNTVQIIHPSNNAVYKIDPNLRLS
                     HQSITFTVTASNQTKRIRWYINGTLYRETHDAFELVWPIKPGKYTLRVQAEQAIQTSE
                     STVHFEVLP"
     gene            3197..3931
                     /locus_tag="PK937_12870"
     CDS             3197..3931
                     /locus_tag="PK937_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33511.1"
                     /translation="MPKLLFIVFTFLMIPASAVEEADRAFYAMDYPLAIRMYETHLNQ
                     KNDSEAYWKLARAYLCSGDISAGVEREVFYRKALTAVESSIQLDRNNSQAYAWFAACL
                     GSVVIFEGSESKIKACYRIKAALDTALMLDPTNDVAWSVLGSYYKALGGINWFERQLA
                     NLFLGGLPEGGYEDAEKALLTAVSLAPDVLRHRFELGVLYYDWDKKDKTQECFEYAMT
                     LKPAMASDNRRISKMKEFMEKHNLPR"
     gene            complement(3947..4408)
                     /locus_tag="PK937_12875"
     CDS             complement(3947..4408)
                     /locus_tag="PK937_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH33512.1"
                     /translation="MRVKLYLLTFYFLISCSAPTSEKTDSEAVKKAIQSANREYMQAY
                     EKSEATRIAALFAEEGVVLQANIEPVRGKSLIERQFVNLFDQSRFTDVRFKTTSIEVG
                     QDMASEYGTYSFSVQPLGLTPATVSGSYLVVWEKQNDGSWKILAEMRQPNI"
     gene            complement(4421..5038)
                     /locus_tag="PK937_12880"
     CDS             complement(4421..5038)
                     /locus_tag="PK937_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33513.1"
                     /translation="MESNSLDFMRAIGAASAVFVPLIVFYLQKRDKLQKTKYFIDLVK
                     SQDELIKIRDQHASQNGNDIVMMKINSLLSEIEDEINHTVDRERIVSFLLFVFLEVIV
                     LSALIIQWSDYLNKIISGASYESGLFFLEGIFQTTTARLILASMIVSLSVFLTIKSVK
                     KWVDRINRPKLRTTMLVLGFNGVLIVVAIVTSLILAILDPIIPLW"
     gene            complement(5062..6453)
                     /locus_tag="PK937_12885"
     CDS             complement(5062..6453)
                     /locus_tag="PK937_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33514.1"
                     /translation="MKLVYLMAVGIYASFITNQSVYADVTPMQTAVGFNLSIPGKSPL
                     MPNRSMAKKETPTKSVGIAMARESVSIVLYPTMMAVHADFEMENLKSDDISMTVGFPL
                     QAALMSGFPMVDFRVLINGVPVQARYEMLTQDIEPGHTVRETWWSFEVTFSKKQKTSI
                     TVEYRQPFIGRSQQRLLHQGPSADSSYVIQAGYVFRTGALWDGLIGEAELNVRLVGLE
                     KNEMLVFFDGVNQWPEDGRWIKKNWEPASDWFVEIAVHPRRISAMRESASYDVTGLSS
                     IAKMEDRLIRFSRMISEAGDSEKVDTQLPSLDAWKDIVEVIKSAAMQNSDTTLKSWAN
                     RCLAIWRQHLLRSMSLSRDATQLAYALEWLNPDQTPSEEMNEFNRKIKTKPEPGYALQ
                     HVPADKHIMFYLTYPDYSNTVFTDSELFLSVVHEKIRGERFLFFGSIFGGMCVIFVAL
                     IVRTIKRRKKTTF"
     gene            6819..7226
                     /locus_tag="PK937_12890"
     CDS             6819..7226
                     /locus_tag="PK937_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33515.1"
                     /translation="MFHEIKNVSQSRGEPFRRWFEDNYFDLVVWYNKNGSFYGFQLSY
                     DRYRHEHAITWFSERGYFHDKVQNTDGSVVTMTPVLVANGPFDGQQIAERFRQHSAGL
                     EIEIAEFIYDKVTHYEQLKPSDTKNKKAGRTPL"
     gene            complement(7254..8825)
                     /locus_tag="PK937_12895"
     CDS             complement(7254..8825)
                     /locus_tag="PK937_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNH33516.1"
                     /translation="MKKKIILPVLLVLVIIVAVTGFTSTGDVYKNLVKLKTIMDLVEQ
                     TYVEDVNGDKMVDDAIAGALKGLDPHTVYIPPKEMKTVKEEFQGNFEGIGISFEIVDG
                     NLTVVTPIPGTPSEKAGLLAGDRIVRIDGDLTKGITNDGVFSKLRGPKGSKVHITIER
                     PGVSEQIEFDIIRDKIPLYSVDAKFMLDDKTGFIHFSKFSETTEEEIEQALRELEAKG
                     MQQLILDLRNNGGGYLEQAQMVVDKFIPGGKKIVYTKGRIPNSTKDYVSSSKSDYRKM
                     PMIILINRGSASASEIVSGALQDLDRALIVGERSFGKGLVQSQYELGDGSALRVTTAR
                     YYTPSGRLIQRPYDGKSIDDYYEEAHEVESVKSDSSHVYFTEMGRKVFGGGGIVPDFH
                     VDYDTNSVYYVKLWSKNIMREFVNQYLEKNGVRLRDTYKSNQNDFIRNFNVPENEIAN
                     LATYGASKGVPVDEKALQLDKQDMLNAVKAEVARYIWGNYEAAQVRLMSDKIVKESMK
                     FFPEARKFLENKKKK"
     gene            complement(8845..>10152)
                     /locus_tag="PK937_12900"
     CDS             complement(8845..>10152)
                     /locus_tag="PK937_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNH33517.1"
                     /translation="NFDGIGSQLDVINGIPTIVTPIPGSPSEKVGLLAGDKILKINDE
                     PTKGFTIDQAVSRLRGPKGTKVRIAVQRTGVNEMLDFEIVRDKIPVYSVDAKFMIDEK
                     TGYIRFVRFAETTEEEIEQALKELEAQGMQQLVLDVRNNGGGYLEEAQRIVDKFIPGG
                     KKIVYTKGRIANASRDYFSSDKTTYRKYPIIILVNRGSASASEIVAGAIQDLDRGIVV
                     GERTFGKGLVQNQFDLNDGSSVRVTTARYYTPSGRLIQRPYDGKSLDQYYGEVRRDTV
                     HSDTTHIYYTASGRKVFGGGGIVPDYHIDNDTVSAYYVKLWGRGIFRDFTNDYLEKNG
                     PKLRDQYKDQFKKFTAGFQISDEDFDALIKMGESRGIAKEADAIVRDKEDMSNFVKAE
                     VARFIWGSYAAAQVRSMTDSAILKTAKYFGEAKKFADQNAKLK"
BASE COUNT         2685 a         2434 c         2184 g         2849 t
ORIGIN      
        1 ttgatttatc gcaaaatgtg aaagataatt cattcattaa cgctttattt atcgtccgcc
       61 ggattttcag cgcgggtatt ggccgcattt tttttagcac taaatcctcc ggctaaggtc
      121 atgacgcaca tataacgata ttgtttatgc gtaccacagg ctacgccgac tttacgaaat
      181 tcaggattaa aaatattttc tctgtggccg cgactcggta cgccgtcatc tacgagcagc
      241 gatatgacaa tacgccgggc gtcattggaa ccatagtcaa tattttcacc ggccgtacgt
      301 tcccattgtc cgtagcgttc gatgcgatca aagggcgaac tcccgtcatc gccggtatgc
      361 ccggttttac cggaacggct ttgttctttc atatgatcgc gagcagcttt ggacataccc
      421 tccgacggtc ccaacgtatc aatgggctgc gcgttttcaa gaaatcggat gcattcatct
      481 acggcgtcaa cgccttcatt ggtcatgagc gtcgcttcgc cgggataata caccatattc
      541 ccgtcatata atttcttgag cgctttcaga tgagtgacat atccttgggg atcacgacga
      601 acacggttaa gctcgcgcac cacttccttt tcgagatccg tgagatacca catcgaaggt
      661 ttagtcggtg tttgtgcatg cacgggagat aatgcgaaat aaagaaaaaa aactcctatc
      721 cctgcagcga gataaccttt gttatacatg tcggaagctc cttttatttt tttgcaatat
      781 cgcaagcccc tcggtgaata gcaaacgtta cctctatatg tttacatttg tttttgaaat
      841 taaatattaa actctctaca ctctgacatg ctcaaaactt tgaaaaaaaa tccggatctg
      901 atttttatcg tattggctgt tatcggaaca tttttttatc cgatttccgg tgatgattta
      961 cattttaggg atgaagcctc cttgcgtatc tatgatcgca acggactttt acttcgtgaa
     1021 gtattatccc gtgaaggggg acgcggtcaa tgggtttcac cggatcagat agatacgcgg
     1081 ataatcaaag cggctatcgc ggcggaagat aaacgttttt tttcgcatcg cggcatggat
     1141 gtactggccc tgggccgtgc tatcggacaa aatatcatgg cgatgtcggt cgtcagcggc
     1201 gggtcaacca tttcccagca agtcatccga aatatctatc attttccgcg caacccgttt
     1261 tacaaactgg tcgaattgtg gtatgcgatt cgcctggaat acacattatc aaaaaatgaa
     1321 attctcgcgc aatatctcaa tcgtatcccg ttcggtaatc aagcttttgg tgtggaagcc
     1381 gcatcccggc tctatctcgg caaatcgtca caaacggtaa catggtcaga ggcggccttt
     1441 ctcatggctt tgcccaaatc gcccacgcgc tataatccct accgcaatga tacggccgct
     1501 aaaaacaggc gcatagccgt tttacagcgc cttcttgtta tgcaggccat tcccaacgat
     1561 gactacgaac gggccgtaag cgaaccaata ctacttcata cgcaacctca taattttttt
     1621 gcaccgcact ttacggatta tctgttacag caccatgccc atcttagcac cgcgtggcac
     1681 accacgctgg atttaccgtt gcaacaatcc gctgaacgca tacttcgcga acatatcacc
     1741 tatctgcaac cggaaaatgt caccaatgcg gcggccatca ttttagataa tcgtacgggc
     1801 gctctacgcg tcatggccgg ttcgtatgat tattttgatg cgcaacacga cggtcaattt
     1861 aatgcggcgc tcgcacggcg gcaacccggc tcggctttga agccttttac atacgcaata
     1921 gcttttgagc gcaactacac cgcggccagc attttagccg acgtacaagc atcgttttca
     1981 acggcgaacg gcacatttac acccatgaac tacgaccgac gttaccatgg gccggtgcgg
     2041 gcgcgtattg ctttggcttg ctcatacaat attcctgccg tacgtgtttt gcaggatgtg
     2101 ggagtacccg ttttattatc aaagctgcat gaatgcggaa tacaaagcct cgatcaatcg
     2161 cctgcatatt acggtcacgg attgacattg ggtaatggtg aagtcacgtt attggaatta
     2221 acacgcgcat tttctgtttt ggccaatcaa ggcgcgctta tccaaacaca agttttttct
     2281 gactcggtgt cccgcgtcga acgcgagcgt atttttacgc cggaaagcat ttttctcgta
     2341 tcggatattt tatccgatcc gatcgcccgc attccggcct tcggttatga ttcgccgctt
     2401 tcattcccat atccggtggc cgccaaaaca ggtacatcca gcgatttcag agataattgg
     2461 acaattgggt atactcgtga ttataccgtc ggtgtgtggg ttggtaattt tgacaataca
     2521 ccgatggacc gcatttccgg tattaccggt gcgggaaaaa tttttcatgc tctcatggat
     2581 gctttatatc cctcgacagc gccgtccggt tttgttgcac cgcatacact ccataaaatc
     2641 ccgatctgtg ccctatccgg acaacgtgcg catcccggct gcggcgcggt gattgatgaa
     2701 tattttatga aaatttcatc ggtgcagacc acctgcacat ggcacgatca acgcggcata
     2761 atccgttatg aaacgatacc gcaagaatac cgcgcatggt ttagcagcgc cggaaatcac
     2821 gcctcggtgc acgcatcggc acccgacaat atttcgaata ccgtacaaat catacatcca
     2881 tcgaataacg ccgtttacaa aatagatcct aatttgcgtt tgtcgcacca aagtataact
     2941 ttcacagtca ccgcgtccaa tcaaacaaag cgcattcgct ggtatatcaa cgggactttg
     3001 tatcgtgaaa cgcatgatgc atttgaatta gtatggccta ttaaaccggg gaaatataca
     3061 ttgagagtac aagccgaaca ggccattcaa accagcgaat ctaccgtaca ctttgaagtg
     3121 ctcccgtaat acaagtattt acgattcgat aacattggcc ttataaattc aaaataactt
     3181 cgtagtaaac tacgctatgc caaaattact ttttatcgtg ttcacattct taatgatacc
     3241 ggcttctgct gtagaggaag cggatcgggc attttatgcg atggattacc cgctggccat
     3301 ccgtatgtat gaaacgcatc tgaatcaaaa aaatgacagc gaagcgtatt ggaagttggc
     3361 ccgcgcgtat ctgtgttccg gggatatcag tgccggagtg gaacgtgagg tgttttatcg
     3421 taaagcgctg accgctgtgg aatcctctat acaacttgat cgcaataatt cgcaagcgta
     3481 tgcgtggttt gccgcatgcc tcggtagtgt ggtaattttt gaaggcagtg aatctaaaat
     3541 caaagcttgc taccggatca aagcggcttt ggatacggcg ctcatgcttg atccgaccaa
     3601 tgacgtcgcg tggtctgttt taggttcgta ttataaagcg ttaggcggaa tcaattggtt
     3661 tgaacgtcaa ttggctaatt tgtttctcgg cggattaccg gaaggcggat acgaagacgc
     3721 ggaaaaagcg ttactcacgg ccgtttcctt agcgccggat gtactgcgcc accgatttga
     3781 actcggtgtt ctttattacg attgggataa aaaagataaa acgcaggaat gctttgaata
     3841 cgccatgacg ctcaaaccgg ccatggccag cgataatcgc cgcatttcca aaatgaaaga
     3901 atttatggaa aagcacaacc tgccgcgttg atcggaatac gccaattcaa atattcggtt
     3961 gacgcatttc cgctaaaatt ttccacgagc cgtcgttctg tttttcccaa accacaagat
     4021 aagagcccga gactgtagcc ggagttagcc ccaaaggctg cacggaaaaa ctgtacgtac
     4081 cgtattctga agccatatcc tgtcctactt cgatggacgt agttttaaat cgtacatccg
     4141 taaaacgtga ttggtcaaat aaattgacaa actgccgctc gatcagtgat ttaccgcgca
     4201 ccggttcgat attcgcctgc aatacgacac cttcttcggc aaatagagca gctatgcggg
     4261 ttgcttcgct tttttcgtag gcctgcatgt attcgcgatt ggcggactga atagcttttt
     4321 taaccgcttc ggaatccgtc ttttcagaag tcggtgcgct gcatgaaatc aagaaataaa
     4381 acgttaacag gtacaactta actcgcatgg tttgctcctt ttaccacaat ggaatgatcg
     4441 gatcaagtat agccaggatc agacttgtaa cgatggccac gacaatgagt acgccattaa
     4501 agccgagcac gagcatcgtc gttctgagtt tggggcgatt gatacggtca acccattttt
     4561 ttacactttt gatcgtgaga aaaacggaaa gactgacaat catcgaagcc aaaatcaaac
     4621 gggccgttgt cgtctgaaat atcccttcaa ggaaaaacag gccgctttca tacgaagcgc
     4681 cggaaatgat tttgttcaga taatcgctcc attggatgat caatgcgcta agcacaatca
     4741 cctccaaaaa aacgaaaagg agaaagctta cgatacgctc tcggtccacg gtatgattga
     4801 tttcatcttc gatttccgat aggagcgaat tgatcttcat catcacgatg tcattgccgt
     4861 tttgggacgc gtgttggtct ctaattttga tgagttcgtc ctgcgatttg accaaatcaa
     4921 taaaatactt cgtcttttgg agtttatccc tcttttgaag gtagaaaaca atcagcggaa
     4981 caaacaccgc cgaagccgca ccgatcgcgc gcatgaagtc taatgaatta ctttccatac
     5041 atttttccgt ttaagtaaaa actagaacgt agtttttttc ctgcgcttta tggttcgcac
     5101 aataagggcg acaaaaataa cacacatccc accgaaaata gatccgaaaa acaaaaaacg
     5161 ttcccctctt attttttcat gcacgacact taaaaacaat tccgaatcgg taaaaaccgt
     5221 attggaataa tccggatatg tcagatagaa catgatatgt ttatccgcag gaacgtgttg
     5281 caatgcatat ccgggctcgg gtttggtttt tattttccga ttgaattcat tcatttcttc
     5341 cgacggcgtt tgatccggat tgagccattc caatgcgtag gccagttgag tcgcatcgcg
     5401 acttagactc atactacgca gcaaatgctg acgccaaatg gctaaacagc gattcgccca
     5461 cgattttaat gtggtatccg aattttgcat tgccgctgat ttgataacct cgacgatatc
     5521 tttccatgca tccaatgaag gaagctgagt atctaccttt tcggaatcac cggcctctga
     5581 tatcatgcgt gaaaatcgta ttaaacgatc ttccattttg gctatggacg acaacccggt
     5641 cacatcatac gaagcggatt ctcgcatggc tgaaatacga cgcggatgaa cagcgatttc
     5701 tacaaaccaa tccgatgccg gttcccagtt ttttttgatc cagcgtccgt cttcaggcca
     5761 ttggtttacg ccgtcaaaaa aaacaagcat ctcatttttt tccaaaccga ccaaacgtac
     5821 attgagttcg gcttcgccga tgagtccatc ccacagagcg ccggtacgaa aaacataacc
     5881 ggcttgtata acatacgacg aatcggccga aggaccttga tgtaataaac gttgttggct
     5941 acgcccgata aacggttgcc ggtattcgac agttatggaa gtcttttgtt ttttggaaaa
     6001 cgtgacttca aatgaccacc atgtttcgcg aacggtgtga ccgggttcga tgtcttgagt
     6061 tagcatttca taacgtgctt ggacaggtac gccattaatt agcacgcgaa aatcaaccat
     6121 cggaaaaccg gacattaaag ccgcctgaag cggaaatccc acggtcatgg atatatcgtc
     6181 ggattttaga ttttccattt caaaatcggc atgcactgcc atcatcgtag gatataatac
     6241 gatcgaaacc gattcacgag ccatagcgat gcccacagac tttgtcggcg tttctttttt
     6301 cgccatactc cggttcggca tcaaaggcga tttgcccgga atggataaat taaaaccgac
     6361 cgcggtttgc atcggtgtta catccgcata tacggactgg ttcgtaataa acgacgcata
     6421 aattccgaca gccatcaagt aaacgagctt cataaatctt tcgagcaaaa aatttttgtg
     6481 aacggctcca atgtagtgga atccaaagta taacgcaata cgtcgaatgt ttcttattct
     6541 tgatattgaa agtgtgtttt caagtccggc aaacggtttt gttttaagtt atcttgagga
     6601 agtcagatac actgaacttt tatacacttt gaataattgc cgttagcgat tgataaaaag
     6661 cccttcattt cactgcattt gtcaataagg tataattata tgtaacattt tgcataaatt
     6721 tcccgcactg taaaaaatta tttgtcactg tgcgaatcat cacagcatcc atccaacaat
     6781 ctgattacta ttttagtaga aaaaacggga ctatcggcat gtttcacgaa ataaagaacg
     6841 tatcccaaag ccgtggagag ccttttcgaa gatggtttga ggataattat tttgatcttg
     6901 tggtatggta taacaagaat ggaagttttt atggctttca attgtcctat gatcgatacc
     6961 gtcacgaaca cgccataaca tggttttccg aaaggggtta ttttcacgat aaggtgcaaa
     7021 acaccgacgg ttcggtggtc actatgacgc ccgtacttgt agccaacggc cctttcgatg
     7081 ggcaacaaat cgccgaacgc tttcgtcagc acagtgcagg attagaaatt gaaattgctg
     7141 aatttattta cgataaagta acacactatg agcaactaaa accatccgat acaaaaaata
     7201 aaaaagcggg gcgaaccccg ctttgataaa cgatcgtacc ggcgatttaa tttttatttc
     7261 tttttcttgt tctccaaaaa tttccgtgct tccgggaaaa acttcatgga ttccttcaca
     7321 atcttatccg acataaggcg aacctgcgcg gcttcgtaat taccccaaat ataacgggct
     7381 acttccgcct taaccgcgtt cagcatgtct tgtttatcca actgaagggc tttttcgtcc
     7441 acgggtacac ccttggaagc gccataggtc gccagattag ctatttcatt ttcaggtaca
     7501 ttaaagttac gaataaaatc gttttgattg gatttatagg tatcgcgtaa acgtacgcca
     7561 tttttttcaa gatattggtt tacaaattcg cgcatgatat ttttgctcca aagtttcacg
     7621 tagtacacgc tattggtatc gtaatccaca tgaaaatccg gaacgatgcc gccaccgccg
     7681 aacactttac gtcccatctc ggtaaaataa acgtgactag agtcagattt gacggattct
     7741 acttcatgcg cttcttcgta ataatcgtcg atcgatttac cgtcataagg gcgctggatc
     7801 agacgaccgc tcggcgtata ataacgcgct gtcgtaacac gcaaagctga accgtctccg
     7861 agttcgtact gcgactgcac taaccctttt ccgaaacttc tttcaccgac gatcagcgcc
     7921 cgatccaaat cctgaagcgc gccggagaca atttcggaag ccgaagccga accacgattg
     7981 atcagaatga tcatcggcat tttgcgatag tccgattttg aactggacac ataatctttg
     8041 gtcgaattgg gaatacgtcc tttggtgtaa acgatttttt taccgccggg gataaattta
     8101 tccacgacca tctgagcttg ttcgagatag ccgccgccgt tattccgcaa atccaagatc
     8161 aactgctgca ttcctttggc ttcgagttcg cgcaaggctt gttcgatctc ttcttcggtt
     8221 gtttcactga atttactgaa gtgaataaaa ccggttttat catccagcat gaatttcgca
     8281 tccacgctat acagcggtat tttatcgcga atgatatcaa attcgatttg ttcagacaca
     8341 ccgggacgtt cgatcgtgat gtgcactttg gaacctttgg gtccacgtaa tttcgaaaaa
     8401 acgccatcgt tcgtaatacc tttggtaagg tcgccgtcta ttctgacgat acgatcgcct
     8461 gccagaagac ctgctttttc cgacggtgta ccgggtatcg gcgtaaccac tgtcagattg
     8521 ccgtccacga tttcaaacga aatacctata ccttcaaaat taccttgaaa ttcttcctta
     8581 acggttttca tctctttggg cggaatgtac accgtgtgcg gatccaagcc tttgagcgct
     8641 ccggcaatcg catcgtccac cattttatcg ccattgacgt cttcgacata cgtctgttcc
     8701 acaagatcca tgatggtctt gagtttgacg aggtttttat acacatcgcc ggtggatgta
     8761 aaacccgtta ccgctacaat gatcaccagc accaaaagca ccggcaaaat tattttcttc
     8821 ttcatatatg ctccattacg accgttattt caatttagcg ttttgatcgg caaatttttt
     8881 ggcttcaccg aaatattttg ccgttttgag aatagcgctg tccgtcatcg aacgcacctg
     8941 ggccgccgca taactgcccc atataaatcg cgctacttct gctttaacaa aattggacat
     9001 atcctctttg tctcgcacga tggcatcggc ttctttggca atccctcttg attcgcccat
     9061 cttaatcagc gcatcaaaat cttcgtcgct gatctggaat cccgcggtaa attttttgaa
     9121 ttgatctttg tactgatcgc gcagtttggg tccgtttttc tcaagatagt cgttagtaaa
     9181 atctctaaaa ataccgcgcc cccagagttt gacgtagtag gccgaaacgg tatcgttatc
     9241 aatgtgataa tccggcacaa taccgccgcc gccgaatact ttgcgaccgg atgccgtgta
     9301 ataaatatgg gtcgtatcgg agtgtaccgt atcacgacgc acttcgccgt aatactgatc
     9361 taatgatttg ccgtcgtaag gacgctgaat aagacgaccg ctcggcgtat agtaacgggc
     9421 cgtcgtgacg cgcaccgatg agccgtcatt taaatcaaat tgattttgta ccaatccttt
     9481 gccgaaggtg cgttcaccga caacgatacc gcgatccaaa tcctggatcg ctccggcgac
     9541 gatctccgaa gccgaggccg agccgcgatt gacaagaatg atgatcggat atttacgata
     9601 ggtcgtttta tcgctggaaa aataatcgcg tgatgcatta gcaatacgac ctttggtata
     9661 tactattttt ttaccgccgg ggataaattt atctacgatg cgttgcgctt cttcgagata
     9721 gccgccgccg ttattgcgta catccaaaac caactgttgc atgccttgcg cttcgagttc
     9781 tttaagcgct tgctcgattt cttcttccgt cgtctcagcg aaacgtacaa aacgtatata
     9841 tccggttttt tcgtcaatca tgaactttgc atccacgctg taaacgggta ttttgtcacg
     9901 cacgatttca aaatcaagca tttcgttgac cccggtgcgt tgtacggcga tacgcacttt
     9961 ggttcctttg ggaccgcgca gtcttgatac ggcctggtct atggtaaacc ctttggtggg
    10021 ttcatcattg atctttaaaa ttttatcgcc ggccaataat cctacttttt cagaagggct
    10081 gccgggtatc ggcgtaacga tcgtcggaat gccattgatc acatcaagct gtgagccgat
    10141 gccgtcaaaa tt
//
LOCUS       DAOQGF010000112        10142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3084_length_10142_cov_7.175573, whole genome shotgun sequence.
ACCESSION   DAOQGF010000112 DAOQGF010000000
VERSION     DAOQGF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_10142_cov_7.175573"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PK937_12905"
     CDS             complement(<1..1259)
                     /locus_tag="PK937_12905"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HNH33518.1"
                     /translation="MNEIAKTVSDPVENDSTGIFNPKSFDKSEHFVNRELSWLEFNRR
                     VLEEAQDPAVPLLERLKFLSIFSTNLDEFFMVRVAGVSRQIDANVLHKGADGLLPRDV
                     MDAMSRRIHEIAIIQHDYFFDVLIPQLQKENIAILKPGELSTEQNNFVRDYFQNTVVS
                     ILTPLAIDPSHPFPHLANRALCLVSEVEPKIESKLPKTNLVVVPISLNVIPRFVRLPS
                     AEGTYQFILLEDLIRTCINDLFIGYEVKNCFTIRVTRDSDLTYDEDPSEDLIKTISES
                     LRNRRKGAAVRLQYERGLPDSLLKLLLRELDLQYEDLYPSRGFICLSDLMNLYQQLDI
                     AALKDPLFPPHHIPELETGADMFSIIRSGDILTHHPYQSFEYVTRFVREAAEDPSVVA
                     IKMTLYRIAANSPIAQALQFAAERGKEV"
     gene            1426..2028
                     /locus_tag="PK937_12910"
     CDS             1426..2028
                     /locus_tag="PK937_12910"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH33519.1"
                     /translation="MIRLFFRLIYFSLAMLGVVVIAAFFLPKHYTVETTIMIKAAPPI
                     VYERAMDLPAWHALAQAAQLPLEKIDGDQLMQLLLQLPSGNGVVGAGVRLDTVRQYIA
                     QGGSALVDKGVNQLKMVCRITETNPPHAFSLRVEGGPFHGLTQSVTLFPTGEGMTTLT
                     MTEQRTVEGFWGGVKAWLSKNGAYQINDRNLSELKRLCEK"
     gene            2077..2367
                     /locus_tag="PK937_12915"
     CDS             2077..2367
                     /locus_tag="PK937_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNH33520.1"
                     /translation="MAEEKKMQEIQIHFPPEKQSGTYANNMVVAHSKEEFILDFMLVT
                     PPTGVVNARIVMSPGHVKRVIAALAQNMRHYEEQFGKIEEASEPKNPFGFQA"
     gene            2370..3227
                     /locus_tag="PK937_12920"
     CDS             2370..3227
                     /locus_tag="PK937_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH33521.1"
                     /translation="MSRIKIIDIHIHIQPWWQLKPEIRTRMAHGKENYDQLLDIMKNP
                     AKLIALMDKADIEKVGLINYVSPDLMGFDDSSNDFSSAYQKYAPDRFIAYGSVHPRFT
                     KDAEADIHRLIHDLGIRAIKVHPPHQLYYPNDYLNGMKNLEIIYRRCEEWGIPVMIHT
                     GTSFFAGARIKYGDPIHVDDVAVDFPNLKIIMAHGGRPLWMETAFYLVRRHKNVSIDI
                     SSIPPKKILTDYFPRLEEIADKVYFGTDWPGPQVDDMRKNADEFLSLPLDETAKLKIV
                     RENALKLFG"
     gene            3287..4147
                     /locus_tag="PK937_12925"
     CDS             3287..4147
                     /locus_tag="PK937_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH33522.1"
                     /translation="MIFIRCIFSSLWLLFVAVVPIFSQEKPVAAILSFHNTSEKFYLD
                     EVAKSFPTLLKTELAQTRSVVIVEREKLDAVLKEQDFVLSDLSEDKDKQAKVGNLIGA
                     DYVITGEVSEAEGRLRIDVAVTRISSGQVIAEKVTGPSRKYVNTMARLLANNIAYELS
                     GEGKKIPSMKLGGAPVTEFAIATLVLGGGAYLAAQKTSDLRAAYRGTTDLAKMNARYK
                     KADRWNKVTISLQVGTAATAIGLVYAFIKNASSDREVLADMDLAEKSNFAVAPYIDSK
                     TSGLNLSFSF"
     gene            4153..4944
                     /locus_tag="PK937_12930"
     CDS             4153..4944
                     /locus_tag="PK937_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33523.1"
                     /translation="MKFIQTYFFILVIIAGNAGCDLKHDNTLDPKNPNAAADQIAVVE
                     NFVIINTNVNVTAPEFNTYSLNALYELEERYDNNLIALEYHMTPNSGGFEDSLAISDN
                     ELRYANEYKGSASRGFPHTFFNGKSVAVQGASSLSVAKQRYQTVLDSVVSNKEKLYVT
                     ATKKIDASQLTLEIQIARYGDEDLTNAKIEYIVYEDKGMPSGHYTVREVRPSETLPLI
                     PAKTIYEFTTDPINIKSSYVQSQIGVVVIIKSALNGRILQAARAF"
     gene            4953..5636
                     /locus_tag="PK937_12935"
     CDS             4953..5636
                     /locus_tag="PK937_12935"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNH33524.1"
                     /translation="MRFNLILLSTITVFCFAACQPIGDSVSADAPVIDGIMAARNTIL
                     VNDTTNITVSARDLNDEQLDYVWTISTNNGGSFEGAINGSTVTYRAPFLPSSYTIKCK
                     VVNESNKSASKDIKITVTNIGTPIVEITQPTDAAFISASAGIYSIKANVKNADASSVD
                     SMVCYIDNKRLSRKTLTNNTTFDWDVTALNGSKRITVQAWARISGPATTDSSVVNVSI
                     EGTISKPGR"
     gene            complement(5666..6739)
                     /locus_tag="PK937_12940"
     CDS             complement(5666..6739)
                     /locus_tag="PK937_12940"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNH33525.1"
                     /translation="MYQSIIRPILFRFDPEVVHETISRGLFASEPLLRHINFLFCNNS
                     SVLSQTLHDINFTNPIGLAAGFDKYGMGVPVWPHLGFGFAEIGTITGQAQDGNPRPRL
                     FRLPENKALINRFGFNNHGADQTAQTLAQWKRENTLHRIPLGINIGKTKIVSLDHAVE
                     DYAYTFSRLFDYGDYFVVNVSSPNTPNLRKLQDKAFLDAIFSSLMKINREQSGSKLLK
                     PLFVKIAPDLTLSQIDDVLSSIENHKLHGIIATNTTITRENINSRYAGETGGLSGFPL
                     RGMSTEIIRYIHKVTSGKLTIIGAGGVFTAEDAYEKIKAGASLIQIYTGFIYEGPRFC
                     YNLNAGLLELMKRDRIKSIRDLR"
     gene            6879..>10142
                     /locus_tag="PK937_12945"
     CDS             6879..>10142
                     /locus_tag="PK937_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNH33526.1"
                     /translation="MTLKLALRNTLKIIIGTVVSVVLLIGLVLAAIQLPWVKNILRGE
                     IERIANKSLYAKLTIGKIEGNFLTHISVFDGRIAFGDTSVLAFESVDVALSPLAIFNK
                     QIQVDHIRIRKPFIHAIGDTNGVINLARLAKPIEPEPVDSAAEPLAGFTINLDEFSIE
                     EGHVKVQLPAFDGALQNLNVRMNVKASPNKQNIKLDQLAFLLTKNKLNKAKETVKTDS
                     LDIKNIGLDVSAHLLARSLYYQINHPEKRDSLWLDINEFRFVTDQSDVYVKGRFLLPD
                     SARGIALHYTADIKTQPLHLSDIRRFAPVQMRNIDKIEFEALATGNDRAATISDLRIH
                     TPAGTLRGFADVHFGADEPLRYVTELEFLGINAAAFADNPDLFTNINGHIRAEGAGIE
                     PKTLTSKLELQLVKSKAYGIGIDRFTINADVQNGKAQLTKFEGITTAGNFNCDGYYNL
                     IDETYHLQTRLRSINIADVVGDTSLQSSINLSLAYDGKGLNPKTAKGQVRVVSDSSKI
                     LGRELQNLTFDASQQNGRLTLNEVKLRTPLANVTASGSVGLDSSVNLKYTMETVDFTL
                     LKKYLGNDSLLKDSLNLKLKYNGSLRGTPNKMTTAGFLTLTNFIFSGITIDSMQFSYF
                     FDNLSPNQLARALDFRREDTTIIGDVFLYTHMVNMEGMVFRDFTTSITKEKGRTLFEV
                     SGSQDTADAFASVRGSLVLENEKRGELFFDELFLKINGKSMRTREVSQKLGEDATIDT
                     TFERWTESWKNSQPVDIFFDIEKNQYEFRNFNLDIGRGSVSLAGKADIAGDQNLDIKL
                     KDLDLSRANALIGSKQSVVEGILNMTASLKGTFEKPVILADWSITKGKASEFTYENFL
                     GNMQYLNRKIQVNMTLNQNSEKSLTMGGYVPIDLSFKDVKERFTNRPMNFKMHSEGID
                     LRFLQSFFGRSLRLNRGDLKVDMTISGNKEKPKLEGEVKIEGGQITFPRSTLGQTFRN
                     ISMYVKLTPERIFLDTLFVQSGKDSRSRLFAQGSVDLGDVLKDFKFENVDKISYLINA
                     HFTDFVPINTKSETSYLHTASISGKMTVKAPTLYYTQISGDLVINNSEIWVVDPRKAK
                     "
BASE COUNT         2950 a         2074 c         2270 g         2848 t
ORIGIN      
        1 acttctttac cgcgctcggc ggcaaactgc aatgcttgcg cgatagggga attagccgca
       61 atacgataaa gcgtcatttt aatcgccact acactcgggt cttctgccgc ttcccgcaca
      121 aaccgtgtga catattcaaa actctgatac ggatgatgag tgagtatatc accgcttcgg
      181 ataatactaa acatatccgc accggtttca agctccggaa tatgatgggg aggaaaaagc
      241 gggtctttga gtgccgcaat gtctagctgt tgatacaaat tcatcaaatc ggaaagacaa
      301 ataaatcctc tggagggata caagtcctcg tattgaagat ccagttcacg gagaagcaat
      361 tttagtaaac tatccggtaa gccgcgttca tattgcaagc gaacagccgc tccctttctg
      421 cggttacgca aagattcgga aatagttttg ataagatctt ccgaaggatc ttcgtcgtaa
      481 gtcagatcgg agtcccgtgt cacccgaatc gtaaaacaat tctttacttc ataaccgatg
      541 aatagatcat tgatacacgt gcgaatcaag tcctccaaca gaataaattg ataagttcct
      601 tccgccgacg gcaatcgcac aaaacgagga atgacattca gggaaatggg aacgacgacg
      661 aggttggtct tcggtaattt actctcaatt ttgggttcga cttcagatac taaacacaat
      721 gcgcgattgg ccaaatgggg aaacgggtgg ctcggatcaa tcgccagcgg cgtaaggatg
      781 gaaaccacag tattttggaa gtaatcgcgt acaaaattat tttgttcggt cgataattcg
      841 ccgggtttaa gtatggcgat gttctccttc tgaagttgag gaatcaacac atcgaaaaaa
      901 taatcatgtt ggatgatagc aatttcatga attcgccgcg acatcgcgtc catcacatcg
      961 cgaggcaaaa gtccgtcggc tcctttatga agcacattcg catcgatctg ccgcgataca
     1021 ccggccacgc gaaccataaa gaactcgtct aaatttgtac taaatataga gaggaacttg
     1081 aggcgttcca gcagaggcac cgccgggtct tgcgcttcct cgagtacgcg ccgattaaat
     1141 tccagccaac tcagttcgcg gtttacaaaa tgttcagatt tatcaaatga tttggggtta
     1201 aagattcccg tagaatcatt ttcgacagga tcggaaaccg ttttggctat ttcgttcata
     1261 acgatacgtg ttttttagca gtgtattaat aagttacgta atcctcgcat gctctgcaag
     1321 gaaagacatc gtttaacata atcataataa ataataagac atgtgttttt gtgattcctt
     1381 agaatgcgct tgcctttagc ggtatggaat tctaacttct cagctatgat acgattattt
     1441 tttcgtctga tatatttttc tttagcaatg ttaggcgtgg tggtcatagc cgcttttttt
     1501 cttccgaagc attataccgt tgaaacaaca attatgatca aggcggctcc gccgatcgta
     1561 tatgaaagag cgatggattt gccggcttgg catgctctgg cgcaagcggc tcaattgccg
     1621 ctggaaaaaa tagacgggga tcaattgatg caattgttat tgcaattgcc atccggaaac
     1681 ggcgttgtgg gtgcgggtgt gcggcttgat acagtgcggc aatatattgc acaaggcggt
     1741 tctgcgctcg ttgacaaagg ggtcaatcaa cttaaaatgg tgtgtaggat taccgaaact
     1801 aatccaccgc atgcattttc actacgtgtt gaaggcggtc cttttcatgg tttgacccaa
     1861 tccgttacgt tattcccaac cggcgaaggt atgacaacgc tgactatgac ggagcagcga
     1921 acggtcgaag gattttgggg cggcgtcaaa gcgtggttat caaaaaatgg agcttatcaa
     1981 atcaatgatc gtaatttgtc agaactaaag cgattgtgtg aaaagtaaat ttttattatt
     2041 taactgatag cgttaagaat aacctctgag gagtttatgg ctgaagaaaa gaaaatgcag
     2101 gaaatccaaa ttcactttcc tcctgaaaaa caatccggaa cgtatgccaa taatatggtc
     2161 gtagcgcact ccaaagaaga atttatactc gattttatgc tggtaacgcc tccgacaggt
     2221 gtcgttaatg ctcgaatcgt tatgagcccc ggtcatgtca aacgtgtcat cgcggccctt
     2281 gcacaaaata tgcgccacta cgaagagcaa ttcggtaaaa tcgaagaagc cagcgaacct
     2341 aaaaatccat ttggttttca ggcgtaatca tgtctcgtat caagattata gatattcaca
     2401 ttcatatcca gccgtggtgg caattgaagc cggagatacg cacgcgtatg gcccacggga
     2461 aagagaacta cgatcaactt ctggatatta tgaaaaatcc ggccaagttg atcgcgttga
     2521 tggataaggc cgatatagaa aaagtaggtt tgatcaacta cgtaagccct gatctgatgg
     2581 gttttgacga ttcatccaat gatttttcgt cggcttatca aaaatatgct ccggatcggt
     2641 ttatcgccta tggatctgta catcctcgat tcaccaaaga tgccgaagcg gatatacacc
     2701 gattgattca cgatctcggc attcgcgcca tcaaggtgca tccgccgcat cagctctatt
     2761 atccaaatga ttatctgaac ggcatgaaaa acttggaaat tatttaccgt cgctgtgaag
     2821 agtggggaat accggtgatg atacataccg gtacgtcttt ttttgcggga gcgcgtatca
     2881 agtacggtga tccgattcat gtggatgatg tagcggtaga ttttcccaat ctcaaaatca
     2941 tcatggcgca cggcggaagg ccgctatgga tggagacggc tttttatctt gtgcgccgtc
     3001 ataaaaatgt gtcgattgat atttcctcca ttcctcccaa aaaaatactg acggattatt
     3061 ttccccgtct tgaggaaata gcggataagg tatattttgg tacggattgg ccgggcccgc
     3121 aggtagatga tatgcgtaaa aacgccgatg aatttctttc tttaccgctc gatgaaaccg
     3181 ccaaactaaa aatagttcgt gaaaatgctt tgaaactttt tggttgaaat tcgtttacct
     3241 atacgtcgtt tcttgaatga acgatattca gaatataggg gtactcatga tctttatacg
     3301 ttgtattttt agtagtttat ggttgctgtt tgttgccgtg gttccgattt tttcacagga
     3361 aaaaccggtg gctgctatcc taagcttcca caacacttca gagaaatttt atttagacga
     3421 agtagcaaaa tcctttccca ccttactcaa aacggaactg gcacaaacgc gctccgttgt
     3481 catcgtcgaa cgtgaaaagc tggatgcggt attgaaagaa caggattttg ttttaagtga
     3541 tctgtccgaa gataaggata aacaagcgaa ggtcgggaat ttgatcggag cggactatgt
     3601 gatcaccggt gaagtttctg aggcggaagg tcgcttacgt attgacgtag ccgttacgcg
     3661 tatttccagc ggtcaggtta tcgcagagaa ggtcacagga ccctcgcgta agtatgtgaa
     3721 tacgatggcg cggttattag ctaataatat agcttacgaa ttatctggcg aaggcaaaaa
     3781 aataccgtct atgaagttgg gtggagctcc ggtcacagaa ttcgccattg ccacgttagt
     3841 ccttggcggt ggagcctatt tagcagcgca aaaaacttct gatttgcgtg cggcgtatcg
     3901 cggtactacc gatcttgcaa aaatgaatgc acgctataaa aaagcagatc gctggaataa
     3961 agtgacgatt tcgcttcagg tcggcactgc agccactgcg atcggtttag tttatgcatt
     4021 tataaaaaac gcttcttccg atcgtgaagt tttggcggat atggatttag ctgaaaaaag
     4081 taattttgct gtcgcaccgt atattgattc taaaacgtca gggctaaacc tttcattctc
     4141 attttgaaaa ctatgaaatt cattcaaaca tattttttta ttttagtgat cattgccggc
     4201 aacgccggtt gtgatttaaa acatgacaac acgctagacc ctaaaaatcc caatgcggcc
     4261 gcggatcaaa tagccgtagt cgaaaatttt gtcattatca ataccaatgt gaatgtcacg
     4321 gctcctgaat tcaatacgta ttctctaaat gcattatacg aattggaaga acgctacgat
     4381 aataatttga ttgcactgga gtatcacatg actcccaatt caggtggatt tgaggattcg
     4441 ctagccatat cggataatga attgcgatac gccaatgaat ataaagggtc ggcttcgcgc
     4501 ggattcccac atacattttt taatggaaaa tctgttgctg tgcagggcgc ttcgtcatta
     4561 agtgttgcca aacaacggta ccaaacggtt ttagattctg tagtttccaa taaagaaaag
     4621 ctgtatgtca cggcgacaaa aaaaatagac gcatcccaat tgacgttaga aatacagatc
     4681 gcacgctacg gcgatgaaga tcttaccaat gccaaaatcg agtatatcgt ctatgaggac
     4741 aaaggcatgc catctgggca ttataccgtt cgcgaagtgc ggccatcgga gacgctgcca
     4801 ctcataccgg ctaagacaat ttatgaattt acgactgatc cgatcaatat caagtccagc
     4861 tatgtgcaat cgcaaatcgg tgtggttgtt attattaagt ctgcgttaaa cggccgtatt
     4921 cttcaagccg cgcgggcgtt ttaggaggaa aaatgcgctt taatttaata cttttatcaa
     4981 caatcactgt tttttgtttt gctgcatgcc aacccatagg ggacagcgtt agtgccgatg
     5041 ctccggttat tgacggtatt atggcagcaa gaaataccat acttgtaaat gatacgacaa
     5101 acataaccgt ttcagcacgc gatttgaacg atgaacagtt ggactatgtt tggaccatta
     5161 gtaccaacaa tgggggatca tttgagggcg ctattaatgg atcaacagta acttaccgag
     5221 cgcctttttt acccagcagt tacacaatca agtgtaaagt cgttaatgag tctaataaaa
     5281 gtgccagtaa agatatcaag attacagtca ctaatatcgg tacacccata gtagaaatca
     5341 cgcaacctac ggatgctgca tttatctctg ctagcgcagg tatttattca ataaaagcca
     5401 atgttaaaaa tgcagatgca tcctcagtgg acagcatggt ttgttatatt gacaacaaac
     5461 gactttcccg caagacgttg actaacaata ccacatttga ttgggatgtt acagctttga
     5521 acggatctaa aaggattacc gtgcaagcat gggcgcgtat tagtggccct gcaactacgg
     5581 attcgtctgt agtgaacgta tctattgaag gaacgatatc taagccgggg cgatgagcat
     5641 tagggcatta tataaaaaaa gttttttaac gtaaatctcg tattgatttg attctatcac
     5701 gtttcataag ttctaataaa ccagcattta gattataaca aaaacgtggc ccctcataga
     5761 taaatccggt gtatatttgt attaacgaag ctccggcttt tattttttca tacgcatctt
     5821 cagctgtaaa aacacctccg gcaccgataa tcgtcagctt tcccgatgta actttgtgaa
     5881 tgtaacgtat aatctccgtt gacataccac ggagtggaaa tccgctcaat cctccggttt
     5941 cacctgcata tctcgaattt atattttcac gcgttatcgt cgtattggtt gcaatgatac
     6001 catgtaattt gtggttttca attgaactta acacatcgtc aatctgtgaa agtgtaaggt
     6061 ccggcgctat tttgacaaac agcggtttaa gcagtttgct accgctttgc tcacggttta
     6121 tcttcatgag ggacgaaaat atcgcatcga gaaaagcttt gtcctgaagt ttgcgcagat
     6181 tgggcgtatt gggtgagctc acattgacga caaaataatc gccgtaatca aacaaacggg
     6241 aaaacgtata agcgtaatct tcgacagcgt gatcaagcga aacaattttt gttttaccga
     6301 tattgatgcc gagaggtatt cggtgcaacg tattttcacg tttccactgc gcaagagtct
     6361 gtgcggtctg atcagcgcca tgattattaa agccaaagcg attgattagt gccttgtttt
     6421 ccggtaacct gaataaacgt ggccgcgggt tgccatcttg tgcttgcccg gttatcgtac
     6481 cgatctctgc aaaaccgaaa cccaaatgcg gccaaaccgg aacgcccatg ccatacttat
     6541 caaaaccggc cgccagacct atagggtttg taaaattaat atcatgtagg gtctgcgaaa
     6601 ggacagagga attattacag aataagaaat ttatatgtct aagtaacggc tcgcttgcaa
     6661 atagcccgcg cgatatggtt tcgtgcacaa cttcagggtc aaatcgaaaa agaataggcc
     6721 tgataatgga ttgatacatg cgttctccga atttacatca tggtgttaaa aaatatacat
     6781 acacggataa aagacaaaac aattttgtag gtaaatcgtt tgaaagtgag aagtattttt
     6841 tgtagtttcc gccacggttt tcattaataa tttttgatat gacattaaaa ctggctcttc
     6901 gcaacactct caaaattata atcggtacgg ttgtatctgt cgtgcttctg atcgggcttg
     6961 ttcttgcggc cattcagttg ccatgggtca agaatatttt gcgcggggaa atcgaacgca
     7021 ttgctaataa atcactctac gcgaaactaa ctatcggcaa aatcgaaggt aattttctca
     7081 cgcacataag cgtatttgac gggcggatcg ctttcggtga tacctccgta ttggcgtttg
     7141 aatctgtaga tgtggccctt tctcctttgg cgattttcaa taaacagatt caagttgacc
     7201 atattcgtat ccgtaaacct tttattcacg ctatcggcga taccaacggg gtaatcaatc
     7261 tggcacgttt agccaaaccc atagaaccgg agccggtaga tagcgcggcg gaacctctgg
     7321 ccggttttac cattaatttg gatgagtttt cgatagagga aggtcacgta aaagttcaac
     7381 tacctgcttt tgacggagca cttcaaaatc ttaatgtacg tatgaacgtc aaagcgtcac
     7441 ccaataaaca aaatattaaa ctggatcagt tagcgtttct tttgactaaa aacaaattga
     7501 acaaagctaa agaaacagtc aaaacggatt cgcttgatat caaaaatatc ggattggacg
     7561 tttcagcgca cttattagcg cgttcattgt attatcagat caatcatccg gaaaaacggg
     7621 attcgttatg gttggatatc aatgaatttc gatttgttac tgaccagtcg gatgtgtatg
     7681 tcaaaggacg attcctgtta ccggacagtg cgcgcggtat tgctttacat tatacagccg
     7741 acattaaaac acagcctttg catctttcgg atattcgcag gtttgcacct gttcaaatgc
     7801 ggaacattga taagatcgaa tttgaagcgc tcgccacagg caatgataga gctgcaacca
     7861 tcagcgatct gcgcattcat acaccggccg ggacattacg tggatttgca gacgtacatt
     7921 ttggcgccga cgaaccattg cgttatgtaa ccgagcttga gtttttgggt atcaatgcgg
     7981 ccgcttttgc ggataatcct gatcttttta ccaatatcaa tggacatatt cgtgctgagg
     8041 gtgcgggtat agagcctaaa actctcacga gtaaactcga actccaatta gtcaaatcca
     8101 aagcttacgg gattggcatt gatcgcttta cgatcaacgc ggacgtgcaa aatgggaaag
     8161 ctcaattgac taaatttgag ggaataacaa cagccgggaa ttttaattgc gatggatatt
     8221 ataatctaat tgacgaaacg taccaccttc aaacacggtt acgttcgatc aatatcgcgg
     8281 atgtcgtcgg tgacacatcg ctgcagagct caatcaatct ttcgctagcc tatgacggaa
     8341 aaggcctcaa ccctaaaaca gccaaaggcc aagtgcgcgt ggtttcggat tcatctaaaa
     8401 ttttaggccg cgaacttcaa aatcttacat ttgatgccag tcagcagaac ggtcgcttga
     8461 cactcaacga agtaaaacta agaacaccgc tggccaatgt tacggcttcg ggttcggtgg
     8521 ggttagattc gtcagtcaac ctcaaataca cgatggagac tgttgatttc acgcttctaa
     8581 aaaaatattt gggtaatgat agtttactca aagattcact gaatttgaaa ttaaagtata
     8641 acgggagcct tcgcggaacg cccaataaaa tgacgacagc gggtttcttg actctgacca
     8701 attttatttt ctccggtatt acaattgatt ccatgcagtt tagctatttt tttgataatc
     8761 tttcgcccaa ccaattagca cgcgcattgg attttcgccg cgaagatacc accattatcg
     8821 gcgatgtttt tttgtatacc cacatggtca atatggaagg tatggtattt agagacttta
     8881 ccacatctat aactaaagaa aaaggccgta cattatttga agtctccgga tcgcaggata
     8941 cagccgatgc ttttgcgagt gtacgcggaa gtttagtttt ggaaaatgaa aaacgaggtg
     9001 agttgttttt tgatgaactc ttcctcaaga tcaacggtaa gtccatgcgg acacgggaag
     9061 tgtcacaaaa actcggcgaa gatgcgacga ttgatacgac atttgagcgg tggacggagt
     9121 cgtggaagaa cagtcagccc gttgacatat tttttgacat cgaaaaaaat cagtatgaat
     9181 ttagaaattt taatttagac attggccgcg gatcagtgag tcttgcgggt aaagcggata
     9241 ttgccggaga tcaaaaccta gatattaagc tcaaggattt ggatttgtcg cgtgccaatg
     9301 ctttgatagg ttccaaacaa tcggttgtcg aaggtattct caatatgacg gcgagcctca
     9361 aaggtacatt cgagaagccg gtgattttgg ccgactggag tatcactaaa ggtaaagcct
     9421 ccgaatttac ttatgaaaac tttctcggaa acatgcaata tctgaatcgc aaaattcagg
     9481 tcaatatgac gctcaatcaa aattcagaaa aatcgcttac gatgggcgga tatgtaccga
     9541 tagatctttc gtttaaagat gtcaaagaac gttttaccaa tcgcccgatg aattttaaga
     9601 tgcacagcga aggtattgat ttgcggtttt tgcagtcttt ttttgggcgg tcgcttcgcc
     9661 tcaatcgtgg tgatcttaag gtggatatga cgatcagcgg caataaagag aaaccgaaac
     9721 tggaaggtga agtcaaaata gaaggagggc aaattacttt tccgcgatca actttggggc
     9781 agacttttag aaatatcagt atgtatgtca aattgacgcc ggaacgtatt tttttggata
     9841 cgttatttgt ccaatctggg aaagattcgc gttcgaggct ttttgcgcaa ggttcggtgg
     9901 atctcggcga tgttctcaag gatttcaaat ttgaaaacgt ggataaaatc agctacctca
     9961 ttaacgctca ttttacggat tttgtaccta tcaatacgaa atcagaaacc tcgtatcttc
    10021 atacggcttc tatatcaggt aaaatgacag ttaaagcgcc cacgctatat tatacgcaga
    10081 tttcgggcga tctggtgatt aataattctg aaatttgggt cgtggaccct cgtaaagcca
    10141 ag
//
LOCUS       DAOQGF010000113        10112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3096_length_10112_cov_7.198270, whole genome shotgun sequence.
ACCESSION   DAOQGF010000113 DAOQGF010000000
VERSION     DAOQGF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_10112_cov_7.198270"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK937_12950"
     CDS             <1..282
                     /locus_tag="PK937_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNH33527.1"
                     /translation="VDGEMQADTAEDATILEKDYPFSTLKGGANVLVFPELQSANAAY
                     KLLQKLGGAETIGPILMGLRYPVHVLQRGCEVNDIVNMTAIAVVEAQEK"
     gene            295..699
                     /locus_tag="PK937_12955"
     CDS             295..699
                     /locus_tag="PK937_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33528.1"
                     /translation="MRLFLNGIFVFILLNLVACTATQQTSYKRKEITMDLLRERFLDK
                     KFAVRMQFYISREELIEETGYLKSAESDKAVLFEYYDARDKQYRNLEVPIQNINTIEL
                     ASKGSDRFSKTQKVAMGSAIVMALLLVFAISI"
     gene            807..2198
                     /locus_tag="PK937_12960"
     CDS             807..2198
                     /locus_tag="PK937_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH33529.1"
                     /translation="MILIDGARYDVFERLLAEGRLPAIQEHIVARGAYRRMTTVFTST
                     TGPAYVPFLTGCFPGKANLPGIRWFDKTAFAEKPFWNPHRFRSYCGWEGFFMNRDINP
                     NIKTIFDLTENPINIFGPITRGILKNNNRGPLKKAWMIARAHSNGVYEPVDDAAFEVC
                     MKTLDEPSAFRFLALPGVDGISHNTFPHHERVLAAYQRADRGIAAIVKKLKSVEQYDQ
                     TVIAICSDHGLSQTHTHFDVPVYMETSLGFKTLFYTNIFRWNPDASAQVSGNGMVHIY
                     FKNGDWRKSCFYEDIIRFPINPVDDFLKHEAVDLVITRMQNGEARVDSRRGTGFIKEE
                     NERILYRVEGKDPFGFEVLPPSMSRNEALARTFETNYPDALVQIVQNFHSPRCGDMML
                     SATIGYDLRFRWENPEHKSSHGSLHREHMMTPFCWSVPITTDHVRTVDVFPSMLKILG
                     KDIPEGVDGVSFV"
     gene            complement(2195..2872)
                     /locus_tag="PK937_12965"
     CDS             complement(2195..2872)
                     /locus_tag="PK937_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33530.1"
                     /translation="MFRIVMTLKKEIHRKFFHFSAVIIPIGYYLTDYATALWCIGALL
                     GIAIVTEYLRLYIPSFSALFNRFFGSMLRDGETDHYSGATFLLIASFLVIFAFHKDVA
                     VLCLLFLIFGDGMAALIGKTIGRTRLLGLDKTLEGALACFAVCLGISFFFGHLPLSVR
                     IAGAFAATVAEIMPMRTSDNLRIPIISGSIMQLMVIQIQQKQPIYLEEKEFVLSFINY
                     FNLFGNV"
     gene            3277..4371
                     /locus_tag="PK937_12970"
     CDS             3277..4371
                     /locus_tag="PK937_12970"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNH33531.1"
                     /translation="MKNSNIVVAVFLMFVCSFETQAQTSEAGVLFLLVPPGARHNGMG
                     QTGVSTANDANAIYYNPGALGFIATQETPRDVQFMHVNWLPQFNLSDLFYDYGSIVMF
                     LPDIGVVGYNFTYFNIGEQQRTNAAGDDLGTIHSYELSTGVTYATKMTEDWGIGGNFK
                     FIYSSLDNKKGTDNNTAEVQNGVGSSVAIDLGVMKKNFFVEDLTFGASLVNVGPSIAY
                     ADEDQADPLPTNLRLGVSYPAYKSEFNNVLVAYEINRQIVRGRSTGSDPLISGNSKFF
                     TRSAIFTTWADNGWHRLGHNIGAEYTYSDFVAMRTGTSLDFAGKLYDLTFGVGIRYDV
                     FKIDFAYSTGLTERFNARDGSQFYSIGVRF"
     gene            4457..7567
                     /locus_tag="PK937_12975"
     CDS             4457..7567
                     /locus_tag="PK937_12975"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33532.1"
                     /translation="MKKVFLFCVLCVVYGVHGQSNLQRAFTSKQEFASKNKTSGTLRV
                     LALRVQFKADNSGQTTGNGRFDLRDLRDSLRIDPAPHDKRYFEDHLLSMRNYFYDVSD
                     GALDITYTVKPNGDTAAYHLSRTMEYYGRIVSRDERDELLAELLHDAITTADTTDGID
                     FSQYDHVIVFHAGVGQDFSLEDATPRDIPSRFVTPSVVDQKYSGGISVNNGAHRVQSG
                     IIVPETESQFFTDAVLGVEVFREIGLNGILIANFGSALGMPDMFNTDQGTPGIGVFGL
                     EDQGAVNGDGMIPAEPDPWTKIYMGWAQPIVVTDTIHAQLQSRKTAGHSVILKVPVSA
                     SEYYLIENRQRDVISNTLSPETLIRIDTIYNVDNTIDEIDTAYMTGVERSPVSGVITK
                     VDEYDSALPGSGLLIWHVDENVIAANLSDNAINNNIERRGIRVVEGSGSQDIGRNFQS
                     FFGTTVGAGDKYDFFFHGNEGFGFYNKKTDSVFFDSKSVPAALTYDRASTGVRITGIS
                     VRQPVMAFDLQSSLQQNGFPQYTGVTIPQAGLTWGNIQGDAKKEIIAVSEKGLVFAWQ
                     ADGSEVIDHDQTITDHRLGQDSVQFAYAWISDFHDSVTGGPAVSDLDQDGYDEIIIAT
                     KNGKIFALRGADGNNDKIADTLFVYLTGQTITTSPMVLPDRRIVVGTSTGDIIILHPN
                     GVFDATLALSSAIRAIVRLENDYLVVATGQHIAKVNLNNMTFTSDAYVPASGSVWLVA
                     GDMDQNGVNSIIAANGKNIHPFYSEPAFTYELPSVISAPPVIGDIDGDEYPDIVFAVE
                     NFVYALNHNGTLVNNFPVRLSDAYRVDQIISSPLLADIDNDGQCDILIAGSDGLIYAV
                     NGRGEKIGGFPLTIGDQAIASPMITDMDGDFDLEMVSVSAEGYVYNFDLPATLTLQTL
                     KWPKYGRDLSLSHLSNESNTPDSVSTGGLMPARSVYNYPNPTRGERTVIRYFLSESAK
                     VTIKIFDLAGDLVTTLHGPGLAQTDNEVVWNLDRVQSGVYFAKVQAKTSSGKKEIRTV
                     KIAVTK"
     gene            7653..8654
                     /gene="purM"
                     /locus_tag="PK937_12980"
     CDS             7653..8654
                     /gene="purM"
                     /locus_tag="PK937_12980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNH33533.1"
                     /translation="MSKVITYKDAGVDIAAGNSTKDQIKKMVRKTYNANVLSEIGLFG
                     GFYRFPKSKYKDPVLVSSADGVGTKLKIAVMTGQHDTVGEDLVNHCVNDILACGAKPL
                     FFLDYLGLGKVHPDVIRNIIIGLTRGCKNNGCALIGGETAQMPGFYADDEYDMAGTII
                     GAVERKNMINGRTIRKGDIMIGLYSSGLHTNGYSLARKVLLEKYKLDQYVEELGCTVA
                     EELLKTHKSYLKPVSAVMDKIQIKGISHITGGGIVENTERIIPKKLKMHMDWSAWQEL
                     PVFGLIRRLGNVPHDDMVRTFNLGVGMILIVSPKDVTKTMQILKSKKEKPFVMGEIG"
     gene            complement(8689..9693)
                     /locus_tag="PK937_12985"
     CDS             complement(8689..9693)
                     /locus_tag="PK937_12985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNH33534.1"
                     /translation="MKVFDNMTQDPIFAESSNENNDNVLPKSRILIVEDDTAVRNMVT
                     EFLSKRSFEIREATNGVEGLRIAMTEKPDAILVDIIMPEMDGLEMIRQLRQHPGTMLI
                     PVIVMTAVSNLETKINVFRAGGDGLLQKPFDLQELEVRIDRAIQISNNFMKLTYVDAL
                     TGVYNRRLFDDRLPVELNRSKRYNQPLALVMVDIDHFKKFNDTYGHRAGDFVLNAVGQ
                     TIKKSLRTQDIVCRYGGEEFCIIMPMTLSKDAAMVMSRIRSDFQDRYFHSPYDKTDFN
                     VNISIGVAQYPNDSSEGDNLVRCADEALYEAKETGRNRVVIYNPASTVFKNRVSKLQF
                     "
     gene            complement(9677..>10112)
                     /locus_tag="PK937_12990"
     CDS             complement(9677..>10112)
                     /locus_tag="PK937_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33535.1"
                     /translation="GGGGPEAVECALYEANQLDWSPGNKGKAIILIGDKPPHGVMDGF
                     EECTSNRDYRIETNRLIEKHVKVYTVLCNNVAETRANFQWLADKTGGKFVTLEAIHDL
                     VNLIVAVSIKESNPLLLQAYTEELRQKGILTDSKRKLLESIR"
BASE COUNT         2759 a         2158 c         2386 g         2809 t
ORIGIN      
        1 gtggacggcg aaatgcaggc ggacacggcg gaggatgcga ccatacttga aaaagactat
       61 ccgttcagta ccctcaaagg cggcgccaac gtgcttgtat ttccggagtt gcaatcagcc
      121 aacgcagcat ataaattact ccaaaagctg ggcggcgcag aaaccatcgg cccgatcctt
      181 atgggcctcc gttatcccgt gcacgtcttg cagcgcggtt gcgaagtcaa cgatatcgtc
      241 aatatgacgg ctattgccgt ggtggaagcg caggaaaaat aaaacaggag acgcatgcgc
      301 ctttttttaa acggtatatt tgtattcata ctattaaatc ttgtcgcatg cactgctacc
      361 caacaaacgt cttataaacg taaagaaatt acgatggact tgttgaggga acgcttttta
      421 gataagaaat ttgccgttcg tatgcagttt tatataagcc gtgaagaatt gattgaggag
      481 acgggttatc tgaaatccgc cgagtccgat aaggccgtgc tgttcgaata ttatgatgcg
      541 cgagacaaac agtaccgcaa tctcgaagtg ccgatacaaa atatcaatac gattgagctt
      601 gccagtaaag gttcggatcg ttttagtaaa actcaaaaag tcgctatggg atcagcgatt
      661 gtgatggcct tattgcttgt gtttgccatt tcgatttagt taaaagaatg tttaagctgc
      721 ccaaaatccg tctgtaattc gtcctacaga tcggatttgg cgcagctttt attttatagg
      781 agatcaaata tattcgttcg attattttga ttttgataga cggcgcacga tacgatgtct
      841 ttgaacgcct tcttgccgag ggccgtctgc cagcgattca ggagcacatt gtagcccgcg
      901 gcgcttaccg tcgcatgacc acagttttca cgtccaccac cggccctgct tacgtcccat
      961 ttctgacagg ttgttttccc ggcaaagcca atttaccggg tattcgctgg tttgataaaa
     1021 cggcctttgc cgagaaacct ttttggaacc cacatcgctt ccgtagttat tgcggatggg
     1081 aggggttttt tatgaaccgg gatattaacc ccaatattaa aacaatattc gatctcaccg
     1141 agaatccgat taacatattc ggcccgatca cacgtggaat tctaaaaaac aacaatcgcg
     1201 gtccgctgaa aaaagcatgg atgatcgcac gggcacacag taatggggtt tacgaaccgg
     1261 tagatgatgc ggcgtttgag gtgtgcatga agacgctgga cgaaccttcg gcgtttcggt
     1321 ttctggcatt acccggtgtt gatggtattt cgcataatac gtttcctcat cacgaacgcg
     1381 tcctagcggc atatcaacga gcggatcgcg gcattgcggc catagtcaaa aaactaaagt
     1441 ccgtcgaaca atacgatcaa accgtcatcg ccatatgcag tgaccacggc ctttcgcaaa
     1501 cacatacgca ttttgatgta ccggtgtata tggaaacatc gcttggtttt aaaacgttgt
     1561 tttatactaa tatttttcgg tggaatcccg atgcgtcggc acaagtttcc ggtaatggca
     1621 tggtgcacat ttattttaaa aacggagatt ggcgaaaatc gtgtttttat gaagacataa
     1681 tacgttttcc cataaatccg gtggatgatt tcttaaaaca tgaagcggtt gatctagtta
     1741 taacgcgcat gcaaaacgga gaagcacgcg ttgacagccg gcggggtacg ggttttataa
     1801 aagaagaaaa tgaacgtatc ttgtatcgtg tcgaaggtaa agatccgttt ggttttgaag
     1861 ttttgccgcc gtctatgtca cgaaatgaag cacttgcaag aacatttgag acgaactacc
     1921 cggatgcgct agtgcagatc gtacaaaatt ttcattcacc ccgatgcggg gatatgatgc
     1981 tttcggcgac gatcgggtat gatctgcgct ttcggtggga aaatccggaa cataaatcgt
     2041 cgcacggttc actgcatcgc gaacacatga tgacgccctt ttgctggagc gttccgataa
     2101 cgaccgatca tgtgcgtacg gtcgatgtct tcccgtctat gttaaaaatt ttaggaaagg
     2161 atattcctga aggtgttgac ggcgtgtcgt ttgtttagac gttgccgaac aaattgaaat
     2221 agtttataaa agacaataca aactccttct cttccaaata tatcggttgt ttttgctgga
     2281 tctgaatcac catcagttgc atgatactgc cgctgatgat cggaatgcgc aggttatcgc
     2341 ttgtgcgcat gggcatgatt tcggctacgg tagctgcaaa tgccccggcg atacgaaccg
     2401 aaagcggcag atgcccgaag aaaaagctta tccccaaaca aacagcaaaa caagccaaag
     2461 cgccttcaag cgttttatct aaacctaaaa ggcgtgtgcg gccgatagtt ttaccgatca
     2521 gcgcagccat accgtctcca aatatcaaaa acaacaaaca caagaccgcg acatctttgt
     2581 gaaatgcaaa gattaccaaa aatgaagcga tgagtaaaaa ggtagccccg ctgtagtgat
     2641 ccgtttctcc gtcgcgcagc atagaaccga aaaaacgatt aaaaagcgca ctaaaactcg
     2701 gaatatataa tcgcaaatat tcggtaacga tcgcgatgcc aagcagcgcg ccgatgcacc
     2761 acaaggccgt agcgtaatcg gtcagataat aaccgatggg tattatcacc gccgaaaaat
     2821 gaaaaaattt gcgatggatc tcttttttta aagtcatgac gatgcgaaac ataagttgtt
     2881 ttttttaata tacgctttag aaccatactt acaatggtgt ttcacaagat tgtgcggcag
     2941 attaataacc cgtaatgaac aaaaaatcgt ttgacagcat catgcgtgtt tcctatattc
     3001 ttcatgtgtt tgattcccta accccctcta tacgaaagga gattttgatc agacaattgc
     3061 tacggtttcg atgacgcgaa agcgatctga ttaaaactca ctcagcctta tttccggttg
     3121 ttgttatacg tcaaagaaac gaaaatttct ttgtattggt ttttttaaat ttattatctt
     3181 acaggaggtt tatcgtagta cactttgcta aagggaaaat caacactaac ttctaagaat
     3241 cttattggag aaaagaaata tatgcaggta tcacgtgtga aaaatagcaa cattgttgta
     3301 gcggtttttt tgatgttcgt ttgttctttt gaaacacaag cccaaacgag tgaagcggga
     3361 gttctctttt tgcttgtacc tccgggcgcg cgtcacaacg gtatgggcca aaccggcgta
     3421 tcaacggcta acgatgccaa cgccatttac tacaatcccg gtgcactcgg atttattgca
     3481 acgcaagaaa cgccacgcga tgtccagttc atgcacgtca attggttgcc gcagttcaac
     3541 ctcagtgatc tattttatga ctacggttct atcgttatgt tcttgccgga tatcggtgtt
     3601 gtcggataca acttcaccta ttttaatatc ggtgaacaac agcgtaccaa tgccgccggt
     3661 gatgatcttg gaacgattca tagctacgaa ttatccaccg gagtgaccta cgctacaaaa
     3721 atgaccgaag attggggtat tggcggcaat ttcaaattta tctatagcag ccttgataat
     3781 aaaaaaggta cagataataa tacggctgaa gttcaaaacg gcgtcggtag ttccgttgcg
     3841 atagatcttg gtgtgatgaa aaaaaacttt ttcgttgaag accttacctt cggggcgagc
     3901 cttgtcaatg tcggcccgag catcgcttat gcggatgaag atcaagcaga tcccttaccg
     3961 actaatttgc gtttaggtgt gtcgtatccg gcctacaaat ccgaatttaa taatgtcctt
     4021 gtcgcctacg aaatcaatcg ccaaattgta cgcggacgca gcaccggttc agatcctttg
     4081 atcagcggca attctaagtt ttttacacgc tcggcgatct ttacaacatg ggctgacaat
     4141 ggatggcatc gtttaggtca taacatcgga gctgaataca cctattcgga tttcgtagct
     4201 atgcgaacgg gtacatcact tgattttgcc ggtaaacttt atgatctgac gtttggtgtc
     4261 ggtattcgtt atgatgtatt caaaattgat tttgcatact ctacgggttt gacggaacgc
     4321 ttcaatgcga gggacggatc gcagttttat agcatcggcg ttcgtttctg atatcaattt
     4381 ttcaatgtat gttcagagcc gaattctccg cgcgtgaatt cggctttttt atatctatca
     4441 atatttctga aaaatcatga aaaaagtttt tttgttttgc gttctgtgtg ttgtttacgg
     4501 agttcatggg cagtccaacc tgcaacgcgc cttcacatcc aagcaagagt tcgcttccaa
     4561 aaacaagacg agcggtacgc tccgtgtttt agcgctaagg gttcaattta aagcggataa
     4621 cagcggacaa accacgggga atggtcgttt tgatttgcga gacctgcgtg attcgctacg
     4681 cattgatccg gcgccccacg ataagcgtta tttcgaagat catttgctga gtatgcgcaa
     4741 ttatttttat gatgtatccg acggcgcgtt ggatattact tataccgtaa agccgaatgg
     4801 ggacacagcc gcatatcatc tttcacggac gatggagtat tatggccgta tcgtttcccg
     4861 cgacgaacgc gacgaacttt tggctgaact tttgcatgat gcgataacaa cggccgatac
     4921 cacggatggc attgattttt cccaatacga tcacgttatt gtttttcatg ccggagtcgg
     4981 acaagatttt agcttggaag atgcgacacc tcgtgatata ccctcacgtt ttgtgacacc
     5041 gtctgtcgtt gatcaaaaat acagcggagg aatttcggtc aataacggtg cgcatcgcgt
     5101 ccaatccggt atcatcgttc cggaaacgga aagtcaattt ttcaccgatg ctgttttagg
     5161 tgttgaagtt tttcgtgaaa tcgggcttaa cggtattttg attgccaatt ttgggagtgc
     5221 gctcggcatg ccggatatgt ttaataccga tcagggaacg cccggcattg gcgtttttgg
     5281 ccttgaagat caaggtgcag tcaacggtga cggtatgatc cctgctgaac ctgatccatg
     5341 gactaaaata tatatgggtt gggcgcaacc catcgttgtt accgatacta tccatgcgca
     5401 actgcaatct cgtaaaacgg cagggcattc ggtaatatta aaagttcctg tttcggcatc
     5461 cgaatactat ctcatagaaa acaggcaacg tgacgttatt tccaatacgc tgtcaccgga
     5521 aacattaatt cgaattgaca cgatatataa tgtggacaat acgattgatg agatagacac
     5581 ggcttacatg acgggagtcg agcgctcacc cgtcagcggc gtaataacca aagtggatga
     5641 atacgacagc gctttgccgg gaagtggttt gctgatatgg catgtggatg aaaatgtcat
     5701 cgcggctaat ctcagtgata acgcgatcaa taataatatc gaacgtcgcg gtattcgcgt
     5761 agtggaagga tccggttcgc aggatatcgg gcgcaatttt cagagttttt ttggaacgac
     5821 cgtcggcgcc ggagataagt atgatttttt ctttcacggc aatgaagggt ttggtttcta
     5881 taataaaaaa acagattcgg ttttttttga ttctaaatct gtaccggccg cactcactta
     5941 cgatcgcgca tcgaccggcg tacgtatcac cggtatcagt gtgcgccaac cggtcatggc
     6001 atttgatctg caatcatcgc ttcaacaaaa tgggtttcct caatatacgg gcgttaccat
     6061 acctcaagcc gggttgacgt ggggtaatat tcaaggcgac gccaaaaaag aaatcatagc
     6121 ggttagtgaa aagggtttgg tctttgcttg gcaagcagat ggatctgaag tgatagatca
     6181 cgatcagacg ataacggatc atcgtttggg tcaagattca gttcagtttg cgtacgcgtg
     6241 gatttcggat tttcatgatt ctgtaaccgg aggcccggcg gtatctgatc tggatcagga
     6301 tggttatgat gaaatcatca ttgcgactaa aaacggtaaa atttttgccc tgcgtggcgc
     6361 tgatggtaat aacgataaaa tagccgatac tttatttgtg tatcttaccg gtcagacgat
     6421 cacaacttcc cctatggtat tacccgaccg gcgaatagtc gtcggaactt cgacggggga
     6481 tatcattatc ttacatccta atggagtttt tgatgctaca ctggcgttga gttcggcgat
     6541 aagagcaatc gtgcgattgg aaaacgatta tttggttgta gcgacaggac aacatattgc
     6601 gaaagtcaat ttgaacaata tgacattcac gtcggacgca tatgtccctg catcaggttc
     6661 tgtttggctg gtagcaggcg atatggatca aaatggtgtc aattccatta tcgcagcaaa
     6721 cggaaaaaac atacatccgt tttattcgga accagcgttt acgtatgaat taccttccgt
     6781 tatttcggca ccgcctgtga tcggtgacat agacggagat gaatatccgg atattgtttt
     6841 tgctgtagaa aactttgtat atgcactcaa ccataatggc acgttggtaa ataattttcc
     6901 cgtaaggctc agtgatgcat accgggtgga tcagatcatt tcctcaccgc ttttagcgga
     6961 tattgataat gacgggcaat gcgatatttt gattgcgggc tcggatgggc tcatctatgc
     7021 cgtgaatggt cgtggtgaga agatcggcgg ttttccgctc acgatcggtg atcaagcaat
     7081 cgcgtcgcct atgataacgg atatggacgg agattttgat ttggaaatgg tttccgtttc
     7141 cgctgaaggg tatgtgtata attttgattt accggcgacg ttgactttac aaacactaaa
     7201 atggccaaag tacggacgcg atctttcgct tagtcacttg agtaatgaaa gcaatacgcc
     7261 cgattcggtc tcgacgggag ggcttatgcc ggcccgatcg gtttataatt atcccaatcc
     7321 gacgcgcggt gaacgaacgg taatacgtta cttcctttcg gaatcggcaa aagtaacaat
     7381 caaaattttt gatttggccg gtgatttagt cacgacactt cacggtcccg gtttggctca
     7441 gacggataat gaagtagttt ggaatctgga tcgcgttcaa agcggcgtat attttgcaaa
     7501 agtacaggct aaaacgtctt ccgggaaaaa ggaaatacgt acggtaaaaa tcgccgtgac
     7561 caaataagag tttttttgat cgttgttttt ggtattggct tttttgtata atcccgcaac
     7621 gagagttttt ttacacagat ggagataatt ttttgtccaa agtaattaca tacaaagatg
     7681 ccggtgtaga tatagccgcc ggtaattcga ccaaagatca aatcaaaaaa atggttcgaa
     7741 aaacgtacaa cgccaatgta ctttcggaga ttggcctatt cggagggttt tatcgttttc
     7801 ccaagagtaa atataaagac ccagtattag tttcgagcgc ggatggcgtg ggtaccaaat
     7861 taaaaattgc cgtgatgacc ggccagcacg ataccgtcgg ggaagatttg gttaatcatt
     7921 gtgtgaacga cattttagct tgcggggcca aaccgctatt ttttctggat tatctcgggc
     7981 ttggaaaagt acatccggat gtgatccgca atatcatcat cggtctgacg cgcggctgca
     8041 aaaataacgg ctgtgcactt atcggcggcg aaacggcgca aatgcccggg ttttatgcgg
     8101 atgacgaata cgatatggcg ggtacgatca tcggtgccgt cgaacgaaaa aacatgatca
     8161 atggccgcac gatacgcaaa ggcgatatca tgatcggact ttattccagt ggtttgcata
     8221 ctaacggcta ttcgctggcc cgcaaggttt tactggaaaa gtataaactg gatcaatatg
     8281 tggaagaatt gggttgtacg gtagctgagg aattattgaa aacgcacaaa agttatttga
     8341 aacccgtttc tgcagtgatg gataaaattc aaatcaaagg tatttctcat attacgggcg
     8401 gcggtattgt agagaatacg gaacgcatca ttccgaaaaa actgaaaatg catatggatt
     8461 ggtcggcctg gcaggagtta cctgttttcg gattgattcg ccgcttaggc aatgtaccgc
     8521 acgacgatat ggtgcgtaca tttaatctcg gcgtcggcat gatactgatc gtgagcccta
     8581 aagacgtaac aaagacgatg cagatattga aatcaaaaaa agaaaaaccg tttgtgatgg
     8641 gggaaatagg ttgatcgaaa aatcaacccc ggtgaaggta tcgaaaaatt aaaactgtag
     8701 tttacttaca cgattcttga aaaccgtcga agccggatta taaatcacca cgcgattgcg
     8761 accggtttcc ttggcttcgt acaaagcttc gtcggcacat cgtaccaagt tatcgccctc
     8821 gctggaatca ttgggatact gggctacgcc gatgctgata ttgacgttga aatccgtttt
     8881 atcgtacggc gagtgaaaat aacgatcctg aaaatcggat cgtatgcggc tcatgaccat
     8941 cgccgcatct ttactcaacg tcataggcat tattatacaa aactcttctc cgccataccg
     9001 gcacacgata tcctgtgttc gcaatgattt ttttatggtt tgtccgacgg cattgagcac
     9061 aaaatcgccg gcacgatgtc cgtacgtatc gttgaatttt ttgaagtggt caatatccac
     9121 catcaccagc gccagaggct gattataacg tttggagcgg ttcagttcga ccggcaaacg
     9181 atcgtcaaac aagcgccgat tgtacacacc cgtcaaagca tccacgtatg ttagtttcat
     9241 gaaattattg gaaatctgaa tggcgcgatc aatacgcact tcgagttcct gtaaatcaaa
     9301 cggtttttgc agaaggccgt cgccgccggc tctgaataca tttattttgg tttcgagatt
     9361 actgacggcc gtcatcacga taacggggat aagcatagta ccggggtgct gacgcaactg
     9421 gcgtatcatt tcaagtccgt ccatttccgg cataatgata tcaacgagta tcgcatcggg
     9481 tttttccgtc atggcaatac ggagaccttc gacaccattg gtagcttctc gaatttcaaa
     9541 cgaacgttta ctcaaaaact ccgtaaccat attgcgcaca gccgtatcgt cttcgacgat
     9601 taggatgcgg cttttgggaa gcacgttatc attgttttcg ttggaggatt cggcaaagat
     9661 cggatcttgt gtcatattat cgaatacttt caagtaattt tcttttcgag tccgtgagaa
     9721 tacctttttg ccgcagttct tcggtataag cctgaagcag aagcggattg gattctttga
     9781 tgctcacggc cactatcaga ttgaccaagt cgtgaatggc ttccaatgtc acaaacttac
     9841 cgccggtttt gtcggcaagc cattgaaaat tggcacgcgt ttccgcgaca ttattacaca
     9901 gcacggtgta cactttaaca tgcttttcga taaggcggtt ggtttcaatt cggtaatccc
     9961 gattgctcgt acattcttca aaaccatcca tcacaccatg aggcggttta tcgccgatca
    10021 aaatgatagc ttttccttta tttccgggtg accaatcaag ttgattggct tcatatagtg
    10081 cgcattcgac cgcttccgga ccgccaccac cg
//
LOCUS       DAOQGF010000114        10108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3101_length_10108_cov_5.374117, whole genome shotgun sequence.
ACCESSION   DAOQGF010000114 DAOQGF010000000
VERSION     DAOQGF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_10108_cov_5.374117"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PK937_12995"
     CDS             complement(<1..674)
                     /locus_tag="PK937_12995"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNH33536.1"
                     /translation="MSKKVFQLAKELLVSHQEIVTFLQKSGFKEVKNHMSGLDEEMIE
                     KVMAKFSKEKKHADTYQRIKDKKEKKDEKQTSIFELSSEPKPEPELEPESVKDEKTVK
                     EVSEPATVHQTEPVPAEPHAVPADETISVEAKNTNADTHPSEKKKVDLLENLKSSQKS
                     IEEFKLAFEKARQQLEKQAMKDETDPRRKKKKGGKEEGEAEPEVLVVEEKGGKNNKKK
                     KKKKNK"
     gene            complement(704..2086)
                     /gene="nusA"
                     /locus_tag="PK937_13000"
     CDS             complement(704..2086)
                     /gene="nusA"
                     /locus_tag="PK937_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNH33537.1"
                     /translation="MEHGGMIVDAFTQIAREKNIEKDELNLVIEEIFKMMIKKKYGST
                     DNFDVIVNLDKPAIEIYQNVVVADPVTNPVAEISIEDAQKMDPDMKVGDDYVHIIDPA
                     SFGRRLIISARQTLNQKIRDIERKLIMDEFKNRIGEIVVGEIRQINKNEIYISFERTE
                     LIMPKMEQISNERYKRSDTVRAVIKDVVDDPRGPRIVVSRAQPEFLQRLFENEVPEIY
                     DGIIEIKAIARDPGERTKIAVFSNDKRIDAVGACVGMKGVRIQAIVKELNNEKIDIVS
                     WSPDAEIFISRALSPAKPLRVEVDTTDKKALAVINDDEMSIAVGRFGQNINLAARLTG
                     FQIETIKYSELSGEAHVEETEDEIVDENETPEVETIRETTVKPTVRNEAKPEAPPEVY
                     TEASLIVEVRGISKAVAEKLLVAGFVKLGDIASIEEIKNVEGVPERAISRLAAVIDNL
                     QPATETSSNE"
     gene            complement(2151..2618)
                     /gene="rimP"
                     /locus_tag="PK937_13005"
     CDS             complement(2151..2618)
                     /gene="rimP"
                     /locus_tag="PK937_13005"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNH33538.1"
                     /translation="MNNLQTRIRNIVSKIIENTEFKIVDIDLKSTSGRYMLRVFLDSE
                     KGIIIDRCREFSREIETVLDAENFLGENYLLEVSSPGLERPISQDWQFRKNMGRTIIM
                     TYLSDQGDKTLEGTVVGVGDNNVTLRVQLAKKQTSDITIELSQIKQAKIKLKW"
     gene            2923..4182
                     /locus_tag="PK937_13010"
     CDS             2923..4182
                     /locus_tag="PK937_13010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH33539.1"
                     /translation="MDLLHKLKQVSEEAERLLAIVEIGKLLNSTIHLDQLLEIILDTA
                     LQNLQAERGTLYLIDLEKKELWSKVLKGEETIRLPIGKGIAGTVAATGETIFLTDAYD
                     DPRFNPEFDKKSGFRTSSMLCTPMKNREGRMIGVFQILNKREGEFEKSDIEFLDTLSL
                     DACIAIENARLHEADLVKQRILKELEVAATIQRMILPKEITSIPGLDLAGLNVPSRQV
                     GGDYYDAVPLPDGRVALVMADVSGKSVPGALLVSTLQASLRAYLESPFELAPLVTKLN
                     RVILKNSTDDKFITFFIGIVDTQKNTLEYLNAGHNPPLLMTKGQLIKLTKGGIPLGMI
                     GFDDYAAQTLNLHSGDTLVLFTDGITEAVSPNDDMYDDARLENLVQNVAHSSAEDLKK
                     IIYNDVMAFVNGAEQADDITLLIAKYQ"
     gene            complement(4196..5488)
                     /gene="hflX"
                     /locus_tag="PK937_13015"
     CDS             complement(4196..5488)
                     /gene="hflX"
                     /locus_tag="PK937_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNH33540.1"
                     /translation="MNKIHDTTRKKERALLVGVAHDRQENRLVDDYLEELALLADTDG
                     VEVVASVKQQLRQIDPSTYIGKGKVHEIVLKVQAENIQVVIFDDDLSPGQTKNIEREA
                     KCKIIDRSALILDIFAKRARTRESKTQVELAQLEYLLPRLSKMWTHLERQGGGGVFTK
                     GPGETQLETDRRLVGQRIAVLKEELKKIERQRETQRAGRGEVFRVALVGYTNVGKSTL
                     LNALSQADVYVEDRLFATLDATTRKVFLDNDHSCLITDTVGFIRKLPHHLVASFRSTL
                     EEVREADILLHVVDLSSPIFMEQMEAVEKVLRELNALKDARIVVFNKVDRVSDEALIT
                     SMRHQFPNSVFISAGRRIGLDRLKQAIKDEIDHAFTLDKVSFDSGDGKFYATVHHLAE
                     VLEETYTDSKTTLHFRALKVNAERIKKLQSERADWDKV"
     gene            complement(5503..6639)
                     /locus_tag="PK937_13020"
     CDS             complement(5503..6639)
                     /locus_tag="PK937_13020"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNH33541.1"
                     /translation="MSEQIICPKCKHQFELSDAFQSRFEAQAEQKIRDELNQKFKSEI
                     AKREEKIRRDATEQWTLQLADLQQQLAIKDKNMQDARTRELQLMKQQREIEEREQNLK
                     LETERLIMEERNKIRIEVEAKAIEAFRLKDAEKDKRIADMMRQVDELKRKAEQGSQQS
                     QGEVMELALENLLREKFPLDVISEVPKGIRGADVLQRVYNRSGLESGTIIWESKRTKE
                     WSPSWIQKLKDDMRSAGANVAVLVTQALPKEIKTMGNLDGVWITGFGTAVELAEVLRV
                     GMTELAQTRIAAVGKNEKMEMLYNYLTGNEFRQKMEAVLEAFVQMRGDLDQERRSMEK
                     NWQRREKQIERMMVSMSRMNGDLEGIMGTALPVIPALELPGDKS"
     gene            6804..8762
                     /locus_tag="PK937_13025"
     CDS             6804..8762
                     /locus_tag="PK937_13025"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNH33542.1"
                     /translation="MSDYFRRIVLCLGVSLIGVLPLRAQGTLYRGTQSLFMETGVGAR
                     AQALGRAFTGLANDGSAVFWNPAGLDFLQQPNFVAYHSTLVEGTFFNFAAVTYPFLEF
                     GSVGVGMARIGVNGIPLNNEWTNLAQGEYSEQEYYIAYGKRLPFWNLSLGTTFKMVNV
                     NASELNANDAPVSKSGFSSGLDIALMWKPDFENAIVRNMSFGFNFQNVVAPSVKLSTF
                     SYNDPRNLRFGIAKEIYFGGDQLKRLTILADVSKGSRSGIEYMAGMEYSFNRFLIARG
                     GFDAGRMTVGMGTEFTQFQRFQVDYSVNLGSQYGAALHRLSLTMNFGKTIEERQNIAR
                     ALRADQDQKMIAQNQEAVKIRAIKEHSTLGAEYLKDGKLIPALVELEQVVSLDPNNAN
                     AKSLLDTVRFRMDKQLADRLADTATSVREMTVTQQQNKFVEDHYRKGYQLVQKGDYIA
                     ALSEFQTALERSPNNPDIQRSLNETRGLLDKRIASIIAKARSNAAGNNFSEALKLLSE
                     ARSLDPTNQQIQREIDTELKRISNRLSFLESTRSGLDAYQKGDYQAAMEAFEAALLVD
                     PQNPTVKEYHKKSIVRAFATFKNLEGDVEKTYLQGVDLYVEGKYEQAIAIWQRILEKD
                     PLNKRVLKAIDKAEDQIRQRDEAKGRKK"
     gene            8769..>10108
                     /locus_tag="PK937_13030"
     CDS             8769..>10108
                     /locus_tag="PK937_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33543.1"
                     /translation="MLFKASLKEELKRPADMKYLVELRDFVVGIGRKYGFDEKVIGTL
                     RLATDEAVTNIIRHAYRDTPGRGVVTLRAIVKTSSLTIIIIDQGKTFDPRNAKAPDMA
                     EYIKIGKKGGLGIFMLKKMMDEIDYQVTSEGNELRLVKYRTQGKRGSRAKWLRDVNLR
                     NRYAIISSAALTLLVLIGYVLYEKIQTKNMENEILKRITAISATMASTSADLMASYDD
                     LSMFQIAQRLKKDNEQMIVDIFVVDRNNYIFAASNRNSLGKYPMPENYETLSFDGSDS
                     PQLPDSVAQQFENPELLTLVKTYKYTQEDLPTFDIATPVYRSNIISQDNLLGYAHVLV
                     REQTILNEMTNLRILGIVLALLVLLVGYTGIAILLARIISPFQKLSDWIRSAGHESVS
                     DDVDIDTTNEVGEIAQAFSDMASKFRKAQIGLVQQKQLQKEIQVAQEIQHMLLPV"
BASE COUNT         2647 a         2384 c         2043 g         3034 t
ORIGIN      
        1 atcttatttt tcttcttctt tttcttttta ttattcttac cgcctttttc ctcgaccact
       61 aaaacttcag gttccgcttc gccctcttct ttgccaccct tcttcttttt acgacgcgga
      121 tcggtttcgt ctttcatcgc ttgtttttca agctgttggc gcgctttttc aaacgcgagc
      181 ttgaattcct cgatggattt ttgcgatgat ttcaggtttt ctaacagatc aacttttttc
      241 ttttctgaag gatgcgtatc cgcgtttgta ttttttgctt caaccgagat cgtttcatcg
      301 gcaggcacag catgaggctc agccggcacc ggttcagtct gatgcactgt tgcgggttct
      361 gaaacctcct taaccgtctt ttcatctttt acggattccg gttctaattc cggttccggt
      421 ttaggctcgg atgacagctc aaaaatagac gtttgttttt cgtctttttt ctcttttttg
      481 tcttttattc tctgataggt atcggcgtgt tttttctctt tggaaaactt agccattacc
      541 ttctcgatca tttcctcatc gagcccgctc atgtgatttt ttacttcttt gaagccgctc
      601 ttttgaagaa atgttacgat ttcctgatgg cttaccaaaa gctctttggc aagctgaaat
      661 acttttttgg acataaatcc gcttttatgt agttaagaaa tacttattca ttggaagacg
      721 tttccgtcgc cggttgcagg ttatcaataa cagcggccaa ccgactaatg gcacgttcgg
      781 gaactccttc gacgtttttg atctcttcga tggaagcaat atcaccaagt tttacgaaac
      841 ctgctaccag taatttttcg gctactgctt tggatatgcc tcggacttcg acgatcaacg
      901 aagcttctgt ataaacttca ggcggcgcct cgggtttagc ctcattgcga accgtcggct
      961 taaccgtagt ttcacgaatt gtttcgactt ctggggtttc gttttcgtca acgatttcgt
     1021 cctctgtttc ttcgacgtgc gcctcgccgc tcaattcgct gtatttaatt gtttcgattt
     1081 gaaaaccggt cagacgtgcg gctaagttta tattttgacc aaagcggccg accgcgatgg
     1141 acatctcatc gtcattgata acagccaagg cttttttatc ggtggtatcc acttcgacac
     1201 gcaagggttt ggccggactc aacgcgcgtg aaataaaaat ttccgcgtca ggactccaac
     1261 tgacaatatc aattttttcg ttattaagtt ctttgacaat cgcctgaata cgtacgcctt
     1321 tcataccgac acacgcgccc accgcgtcaa tacgcttatc gtttgaaaat acggcgattt
     1381 tcgtacgctc acccggatca cgggcaatag ctttgatttc gataatacca tcatatattt
     1441 caggtacttc gttttcaaac aaacgttgca gaaattcggg ttgcgcacga gataccacga
     1501 tacgcggccc acgcggatca tccacgacat ctttgataac ggcacgcacc gtatcactgc
     1561 gtttgtaacg ttcattggaa atttgttcca ttttgggcat gataagttcg gtgcgctcaa
     1621 aactgatata aatttcattt ttgttgatct ggcggatttc accgacaacg atttcgccga
     1681 tacggttttt aaattcgtcc atgatcagtt tgcgctcgat gtctcgtatt ttctgattga
     1741 gtgtctgacg tgcactgata ataagacgac gaccgaaact tgccggatca atgatatgca
     1801 cataatcgtc gccgactttc atatccggat ccattttttg agcatcttca atggaaatct
     1861 cggccacagg atttgttacc ggatcggcaa cgacaacatt ttggtaaatt tcaattgccg
     1921 gtttgtctaa gttaacgatc acatcaaagt tgtccgttga gccgtacttt tttttgatca
     1981 tcattttaaa aatttcctcg atgaccaaat tgagttcatc cttctcaatg tttttttcac
     2041 gggcaatctg cgtaaacgca tcgactatca tgccaccgtg ttccatgctg cctccgataa
     2101 atggttcact atattaaatt aatttgtttt ttaatttatt ctttttacaa ctaccacttc
     2161 agtttgatct ttgcctgttt gatttggcta agttcaatag tgatatctga agtttgtttt
     2221 tttgctaact ggacacgaag cgtaacatta ttatctccca cgccaaccac tgttccttcc
     2281 aatgttttat cgccttggtc cgataaatat gtcattatta ttgttctgcc catgtttttg
     2341 cgaaattgcc aatcttgtga gatcggacgc tccaaaccgg gagacgaaac ttcgagaaga
     2401 taattttcac ctaaaaaatt ttccgcatca agaactgtct cgatttcacg gctaaattca
     2461 cggcagcggt ctattattat ccctttttca ctatcgagaa atacgcgcaa catataacga
     2521 ccggatgtgg atttcaaatc aatatccaca attttaaatt cagtattttc aataatttta
     2581 ctgactatgt ttcgtatacg cgtttgtaaa ttattcactt ttataacttt atgaaaacaa
     2641 aaaagtgggt cttgcgaacc cactccgtaa taggtaaaga agctgtccgt aaatataaag
     2701 cttcttttta cgtaatgcaa gtattttttg attcaacttt gtgagactat tgatgttgtt
     2761 ttttaaggat ttgtaaattt aatctgactt gagttcatat gaaaaaaaat tcaaaacaac
     2821 tatctttcat actttcttgc gctctgtatc atactcgatg ttaattttgt tgaattaaac
     2881 taattattgc ttatttttgg aatacactta taggtttaca ttatggattt gcttcacaaa
     2941 ctcaaacaag tttcggaaga agccgagcgc ttactggcta tcgtcgaaat cggaaaatta
     3001 ctcaattcga caattcatct ggaccagttg cttgagatta tattggatac cgcattacaa
     3061 aatcttcagg cggaacgcgg aacattgtat ctgattgatc ttgaaaaaaa ggaattgtgg
     3121 tccaaagtac tcaaaggcga agagaccata cggcttccta tcggcaaagg tattgcggga
     3181 accgttgcag ctacaggaga aacgatattc ctgaccgatg cctatgacga tccccgattt
     3241 aaccctgaat ttgataaaaa atcaggattt cgcacgagca gtatgttatg cacaccgatg
     3301 aaaaacagag aaggccgaat gatcggtgtt ttccaaattc tcaataaacg tgaaggtgaa
     3361 tttgaaaaat cggatatcga atttttggac acgttatcac tcgacgcatg tatcgcgatc
     3421 gaaaatgcgc ggttgcatga agccgatctc gtaaagcagc gcattctaaa ggagcttgaa
     3481 gttgcagcca ctattcagag aatgatatta ccaaaagaaa tcacctccat tcccggtctt
     3541 gatctggccg gattgaatgt ccccagtcga caggtcggag gagattacta tgatgccgtt
     3601 cccctccccg acggacgggt ggctttggtc atggccgatg tatccggtaa aagcgtaccc
     3661 ggtgcattgc ttgtatccac tctacaagct tcgttacgcg cctatttgga aagccctttt
     3721 gaacttgcac cgctcgtaac caaactcaat cgtgtcatac tcaaaaattc gaccgatgac
     3781 aaatttataa cttttttcat cggcatagta gatacacaaa aaaacacgct cgaatacctc
     3841 aacgcaggac acaaccctcc gcttttgatg accaaaggtc aattgatcaa gttgactaaa
     3901 ggaggtatcc cgttaggtat gatcggcttt gatgactatg ccgcgcaaac gttaaatctt
     3961 cattcaggtg atacgttggt cttattcacc gacgggatta ctgaagctgt ttcccctaat
     4021 gacgatatgt atgacgatgc acggttagaa aatttagtac aaaatgtcgc gcattcttcc
     4081 gcggaagatc ttaaaaaaat catctataac gacgtaatgg cttttgtgaa cggtgccgaa
     4141 caagccgatg acatcacatt actaatagca aaataccaat aaactcagct cgttctcaca
     4201 ctttatccca atctgctctt tcggattgta gtttttttat acgttcggca ttgaccttta
     4261 atgcacggaa atgaagtgtc gttttagaat ccgtgtacgt ctcttccaaa acttccgcta
     4321 gatggtgtac ggtcgcataa aacttaccat caccgctatc aaacgaaact ttatccaacg
     4381 tgaacgcatg atctatttcg tccttgatag cttgtttcag tctatccaac ccgatacggc
     4441 gaccggccga tatgaacacg ctattgggaa actgatgacg catactcgtg atcagcgctt
     4501 catcgctcac ccgatccact ttattaaata cgacgatgcg cgcatctttg agcgcattta
     4561 attcacgcaa tactttttcg accgcctcca tttgttccat aaaaatagga ctgctcaaat
     4621 ccacgacatg caataaaata tctgcttcgc gtacttcttc gagcgtggag cgaaatgaag
     4681 cgaccaagtg atgcggcagc tttctgataa aaccgaccgt atctgttatc aaacaggaat
     4741 gatcgttatc taaaaacact ttgcgcgtcg tcgcatcaag cgtagcaaaa agcctgtcct
     4801 ccacgtacac atcggcctgt gaaagtgcgt tgagtaatgt agatttgccg acattggtat
     4861 atccgaccaa agctacacga aaaacctcac cgcgtcccgc ccgttgggtt tcccgctgtc
     4921 gttcgatttt tttaagttct tcctttaaaa cggcaatacg ttgtccgacc aaacgccgat
     4981 ccgtctcgag ctgcgtttca ccggggcctt tggtaaaaac cccgccgccg ccttgccgtt
     5041 ccaagtgagt ccacatttta gaaagtcttg gaagcagata ttcgagttgt gccaattcaa
     5101 cttgggtttt cgattcgcgc gtgcgtgcac gcttagcaaa aatatcaaga atcaacgcgc
     5161 tccgatctat gattttacat ttcgcttcac gctcgatatt ttttgtttgg cccggagaaa
     5221 gatcatcatc aaatataact acctgaatat tctcggcctg cactttgagc acgatctcgt
     5281 gcaccttccc tttaccgata tatgtggatg gatcaatttg ccgaagctgc tgtttcacgg
     5341 atgccaccac ttccacaccg tcggtatccg ctaacaaagc aagttcttca agatagtcat
     5401 ccactaacct gttttcctgg cgatcatgtg caacgcctac aagtaatgcg cgttcttttt
     5461 ttcgtgtcgt atcgtgtatt ttattcatat tctcctaaaa ttttatgatt tatccccagg
     5521 taattccaac gccggaataa ccggtaaagc cgtgcccatg atgccttcta aatcgccatt
     5581 cattcggctc atgctcacca tcatgcgttc aatttgtttt tctcgacgtt gccagttctt
     5641 ttccatgctg cggcgctcct gatctaaatc gccgcgcatt tgcacaaaag cttcaagtac
     5701 agcctccatc ttttggcgga actcattgcc cgtaagatag ttgtataaca tttccatttt
     5761 ctcattttta ccaaccgccg ctatacgtgt ttgcgcaagc tccgtcattc ccacacgcaa
     5821 aacttcagcc agttcgaccg ctgttccaaa tcctgtaatc cagacgccgt ccagattgcc
     5881 catcgtttta atttctttcg gtaaggcttg ggtaaccaaa acggctacat tggctcctgc
     5941 cgaacgcata tcatctttga gcttttgaat ccacgatggg ctccattctt tggtacgttt
     6001 cgattcccat attatggttc cggattccaa gcccgatctg ttgtaaaccc gttgcaaaac
     6061 atcggcgccc cgaattcctt tgggaacttc gctgataaca tcaagcggaa atttttcacg
     6121 tagtaaattc tccaaagcca gttccatgac ctccccttgg gattgctgtg aaccttgctc
     6181 cgctttgcgt ttgagttcgt ccacttgtcg catcatatcg gcaatacgtt tatctttctc
     6241 agcatctttg agtcgaaatg cttcgatcgc cttcgcttcg acttcgatac ggattttatt
     6301 acgctcttcc ataatcagac gttcggtttc caacttcaaa ttttgttcgc gttcttcgat
     6361 ctcgcgttgc tgtttcatca attgcaactc gcgggtacgg gcatcctgca tgttcttatc
     6421 tttgatagcc agttgttgct gcagatcggc caactgcaat gtccattgct ccgtcgcatc
     6481 acgacgaatc ttctcttcac gtttggctat ttcggactta aatttctgat tcaattcgtc
     6541 ccttattttt tgctcggcct gcgcttcaaa gcgactttga aaggcatctg aaagttcaaa
     6601 ctgatgcttg catttgggac atataatctg ctcactcatg ataactcctt acataaacaa
     6661 cgccgccatg atacgatgat ttgcagtgat tttctatcat attttttcac aattttttct
     6721 attgcgtctt cgagcgtata actgtatttt tattgaaaat agcacacttc ggttttttca
     6781 cgtttcaagt aaagcgcttg cccatgtcgg attatttccg ccgtatagtt ttgtgtctgg
     6841 gtgtatcttt gataggtgta ttgccgcttc gcgcacaagg cacgctctat cgtggcacgc
     6901 agagtctttt tatggaaacc ggtgtcggcg cacgcgcaca ggctttgggt cgtgcattta
     6961 cggggctcgc caatgacggt tcagccgttt tttggaatcc agccgggtta gattttttgc
     7021 aacagcctaa ttttgtagcc tatcattcca cccttgtcga gggtactttt ttcaattttg
     7081 ccgccgtaac atatcctttt ttagaatttg gctccgtcgg agtcggtatg gcgcgtatcg
     7141 gggtcaatgg catccctctc aataacgagt ggactaatct ggctcaaggc gagtactcag
     7201 aacaagaata ctatatcgca tatggcaaac gcctaccatt ttggaattta tcactaggta
     7261 caacttttaa aatggtcaat gtcaatgcca gcgaactgaa tgccaatgat gcgccggttt
     7321 cgaaatccgg attttcaagc ggtctggata tcgcactgat gtggaaacct gattttgaaa
     7381 atgcaatagt acgcaatatg tcttttggtt ttaattttca aaatgttgtc gctccctctg
     7441 tcaaactttc cacattttcg tacaacgatc cccgcaatct gcgttttggt atagccaaag
     7501 aaatttattt tggcggtgac caactcaaac gactaactat ccttgcggat gtgagcaaag
     7561 gttcacgttc cggaatagaa tatatggcgg ggatggaata ttcgtttaat cgttttctga
     7621 tagctcgcgg cggttttgac gccggacgta tgaccgtggg tatgggcaca gaatttacgc
     7681 agtttcaacg ctttcaagtg gattattctg tcaatctcgg gagtcaatac ggggcagcgc
     7741 tgcatcgcct ctcgcttacg atgaattttg gtaaaaccat cgaagagcgc caaaatattg
     7801 cccgcgcgct tcgcgccgat caggatcaaa aaatgatcgc acaaaatcag gaggctgtca
     7861 aaatccgtgc gatcaaagaa cattcgacgc tcggcgctga atatctgaaa gatggcaaac
     7921 tcatacccgc gcttgttgag ctggaacaag tggtctcctt ggatcctaat aatgcgaacg
     7981 caaaatcttt attagatacg gtgcgtttca ggatggataa acaattggcg gatcgcttag
     8041 ccgatacggc gaccagtgtc cgcgaaatga ctgttaccca acaacaaaat aaatttgtcg
     8101 aagatcacta tcgcaaaggg tatcaattag tacaaaaggg agattacata gccgccctga
     8161 gcgaatttca aacagcgctt gagcggtctc ccaataatcc cgatattcag cgttcactca
     8221 acgaaacacg tggtcttctt gataaacgaa ttgccagtat tatcgctaaa gcccgatcca
     8281 atgcggccgg taataatttc tccgaagcgc taaaattact gagtgaagcg cgcagccttg
     8341 acccgaccaa tcagcaaata cagcgtgaaa ttgacacaga actcaaacga ataagcaatc
     8401 gccttagttt cttggaaagc acccgtagtg gtcttgatgc gtatcaaaaa ggtgattatc
     8461 aagcggctat ggaagctttt gaagctgcgc tgctcgttga cccacagaat ccgacggtca
     8521 aggaatacca caaaaaatcc attgtcaggg ctttcgctac attcaaaaat cttgaaggcg
     8581 atgtagaaaa aacatacctg cagggtgtgg atttgtatgt cgaaggaaaa tacgagcagg
     8641 ccatagcgat ttggcagcgc attttagaaa aagacccgct caataaacgc gtattaaaag
     8701 ccatcgataa agccgaagat caaattcgtc aacgcgatga agccaaagga cggaaaaaat
     8761 aaattcctat gctttttaaa gcttcactaa aagaagaact caaacgcccg gctgatatga
     8821 aatatctggt cgaactgcgc gacttcgtcg tcggcatcgg gcggaagtac ggctttgatg
     8881 aaaaagtcat aggcacattg cgcctcgcaa cggatgaagc cgttacgaat atcattcgac
     8941 atgcgtatcg tgataccccg ggccgcggcg tagtcactct tcgggcgatt gtaaaaacat
     9001 ccagccttac cattatcatt atcgatcagg gcaaaacatt cgaccctcga aacgccaaag
     9061 cgcctgatat ggctgagtat atcaaaatcg gcaaaaaggg cggtcttggt attttcatgc
     9121 tcaaaaagat gatggatgag attgattacc aggtcacttc cgaaggtaac gaacttcgcc
     9181 ttgtcaaata tcgtacgcaa ggtaaacgcg gcagccgcgc caagtggctc cgtgatgtga
     9241 atttacgtaa ccgctatgct attatatcat ccgccgctct cacgcttctc gtattgatcg
     9301 gatatgtgtt atacgaaaaa atacagacga agaacatgga gaacgaaatc ctcaaacgta
     9361 tcacggcgat ttcagcaact atggcttcaa caagcgcgga tctcatggcc agttacgacg
     9421 acctttccat gtttcagatt gcgcagcgtt tgaaaaaaga caacgaacag atgatcgtgg
     9481 acatttttgt agttgaccgc aacaactata tttttgctgc ttccaatcgc aatagcctcg
     9541 gcaaatatcc aatgcctgaa aattatgaaa cactatcttt cgacggttcg gattctcctc
     9601 aacttcccga tagtgttgcg caacaatttg aaaaccctga gctacttaca ttagtaaaaa
     9661 cctataaata tactcaggaa gacttgccca cgttcgacat tgcaacaccg gtttaccgtt
     9721 ccaatatcat ttcgcaagac aatttgctcg gctatgccca cgtattggtg cgcgaacaaa
     9781 ctattctcaa tgaaatgacc aatttgcgca ttttagggat tgtcctggca ttacttgtac
     9841 tccttgtcgg ctataccggt atcgctattc ttctcgctcg cattatcagt cccttccaaa
     9901 aactaagtga ctggattcgc agcgccggcc atgaatccgt ttcggatgac gttgacattg
     9961 atacgaccaa tgaagtcggt gaaatcgcgc aggctttttc cgacatggca tccaaatttc
    10021 gtaaagctca gataggtctc gtacagcaaa agcagttgca aaaagaaata caggtcgctc
    10081 aagaaatcca gcacatgttg ttaccggt
//
LOCUS       DAOQGF010000115        10039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3131_length_10039_cov_5.756611, whole genome shotgun sequence.
ACCESSION   DAOQGF010000115 DAOQGF010000000
VERSION     DAOQGF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_10039_cov_5.756611"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..339
                     /locus_tag="PK937_13035"
     CDS             100..339
                     /locus_tag="PK937_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33544.1"
                     /translation="MRNGFLFPIIKFTSTPTLKRGTSKNTLRVIAKGSKFDLYFNDQF
                     LAAFEHEAIEKGGFGIWSGQGSHISVDNVKIWNIK"
     gene            complement(360..1535)
                     /locus_tag="PK937_13040"
     CDS             complement(360..1535)
                     /locus_tag="PK937_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH33545.1"
                     /translation="MSANDEFALNVEKAVTERYTEGAQTTVAALCCPVSYDPKYLQIL
                     PQEILDRDYGCGDPSRYVRPGETVLDLGSGGGKICYIAAQIVGSEGRVLGVDMNSEML
                     ALARKYKDPIGQKLGYTNVTFHRGKIQDLKLDWDVLDAYVRENPIQHGDDLQRLELFT
                     QELRNQRPMISDNSVDVVLSNCVLNLVREEDRQNLIAEIYRVLKPGGRFAISDIVSDE
                     DIPLQQKNNPELWSGCISGAFREDEFLKVFEKAGFYGISLDKWETTPWQVVEGIEYRS
                     VTVIGYKGKEGPCLERQQAVIYKGPWKAVIDDDGHTLFRGERMAVCDKTFGIYTREKG
                     PYANDIFAVNPKEIISLESAKLFNCNKNARRHARETKGMDYNETILSEACCGPDGCC"
     gene            complement(1553..2668)
                     /locus_tag="PK937_13045"
     CDS             complement(1553..2668)
                     /locus_tag="PK937_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNH33546.1"
                     /translation="MAMLILLFAGAFFVAYTNGANDNFKGVATLYGSKTLGYKASIGF
                     ATVATFAGSVCSIFIAQSLVSNFSGKGLVPDTLTVAPEFLTAVALGAGLTVLIATFFG
                     FPISTTHGLTGALTGAGFLAVGPQLNTEKLGSAFFIPLLVSPLVSVVVAVIVYVVFRQ
                     IRIWAGIREEHCICVGTCQTVIPTPRPAHALSFQSVTLPEITFGDEASCQRRYGGAVM
                     GISAQRMVDALHYISAAIVSFARGLNDTPKIVGLVFAAQIIRIEWSMIAIAIAMAIGG
                     LLNARKVARTMSENITSMNHGQGLTANLVTGFLVIFASRWGLPVSTTHVSVGAISGIG
                     IVTRQADFKVIRQVVLSWALTLPVAAVIAASIYYLIQ"
     gene            complement(2707..3684)
                     /gene="arsS"
                     /locus_tag="PK937_13050"
     CDS             complement(2707..3684)
                     /gene="arsS"
                     /locus_tag="PK937_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560827.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNH33547.1"
                     /translation="MKYSFHEKLREHELTLPPLGIHTLQVNITKLCNQACRHCHVDAS
                     PKRTEQMDRLTVEHCLAILAAHDEIKNLDITGGAPELNPHFEFFVTEARRLGKHVMVR
                     HNLTVSLDPHPLTKQPMTHLPQFFADHGIEVISSLPFYEEYFTDKQRGRGVFNKSIAA
                     LKNLNALGYGKDDSGLVLNLVYNPVGTYLPAAQGALEADYKRSLHDKFGIHFNNLFTI
                     TNMPIHRFREDLERTGKYDEYMMKLINAFNPGAAQGVMCRTLISVSYDGKIYDCDFNQ
                     MLGMQSHDHEPLSVFNFDATRIRNRAILFASHCFGCTAGSGSSCGGTTA"
     gene            complement(3729..4061)
                     /locus_tag="PK937_13055"
     CDS             complement(3729..4061)
                     /locus_tag="PK937_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNH33548.1"
                     /translation="MNYYESEDLKKFGDVGRFRKELMDKFFAYYNEVTGKDGALTKRE
                     KALIALAVAHAKNCPYCIDAYTTQSLECGASPDQMTEAVHVASAMMAGITLVHGVQML
                     NNLDNLNV"
     gene            complement(4160..5332)
                     /locus_tag="PK937_13060"
     CDS             complement(4160..5332)
                     /locus_tag="PK937_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNH33549.1"
                     /translation="MKSFQILLPVVLIAAFLAGSGCADLIEPLDSIDGNDTPETAILF
                     PRLPQVQQSTVSVSDDIDYFRFVLSSPRHVAVTLAVPDDKDYNLYLMDSRDSVVGLSR
                     GANGITESIDIPHLNAGTYFIKILSENGAYSETQLYRLSIADADTGQTPNLHLSLGNP
                     SQSETDITSPRNYLIARSQYAVSYDNIRRIPNWCAWHLSKNWLGTIARQDNFKTDVLP
                     SGWYAVKDGDYTGSGYDRGHMCPSGDRNAVPSDNVSTFYMTNILPQTSDNNQGPWVEL
                     ESYCRDLAGEDYELFIMSGHYDSLKTIASGKVTVPVRVWKIIVVLPHEETTPDQVTTL
                     TRVIAVDMPNTDGIRSTSWKSYRTSVDQIETSTGYDFLNRVPAEIQSVIESQVDNK"
     gene            5495..6139
                     /locus_tag="PK937_13065"
     CDS             5495..6139
                     /locus_tag="PK937_13065"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNH33550.1"
                     /translation="MRVDRIIEAFLPREDKFFKLFEETAQHLITASDLMVKLMNCPAE
                     ERLAVSQEIHKLEHLCDDTAHRLFSELNATFVTPFDREDIMMLASALDDVMDFLDESA
                     RRIILYKITSIPANMKQLADVLKESITETHHGVSHIRNIHKTEGIRKTLERIHACENK
                     ADMVFEMGIADLFDNEKDPINLVKLKDIYTSLERSTDKCEDVANVLEAILIKHA"
     gene            6153..7148
                     /locus_tag="PK937_13070"
     CDS             6153..7148
                     /locus_tag="PK937_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNH33551.1"
                     /translation="MTELLFITIALALIFDFINGFHDAANSIATVVSTRVLSPFVAVL
                     WAAAFNFLAYWLFELHVADTVAKTVKPEAITLVVVASGLVGAIIWNLLTWWLGIPSSS
                     SHTLMGGFAGAAIAYSHGDFSVVRMEKISPVLYFIFLAPVIGMIVSYIISVITIHICK
                     RQSPHKVDKIFRRLQLLSSAAFSLGHGGNDAQKVMGIIAAAMLSQGIIADMHHIPEWV
                     PLACYGSIALGTMMGGWRIVKTMGQKVTKLSPFEGFAAETAGAVTLFGTGYTGIPVST
                     THTITGSIIGVGVIKRVSAVRWGVTRNLLWAWVLTIPISGLFGAICYWLASFFVS"
     gene            7298..9001
                     /locus_tag="PK937_13075"
     CDS             7298..9001
                     /locus_tag="PK937_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH33552.1"
                     /translation="MKNRRNISYLALIGITIFLYGCSSKSQRTTPPKPPRLIVFIALD
                     QFRYDYLTRFSTHFGANGFNYLLNGGASFANCLYEQATTHTSVGHATMMSGTLPYRHG
                     IIANYWWSRELHRYIYAPEDTLAPILGIEKQKATDGRSPRNFSGINFADALREKTNGR
                     AKVFSVSNKDRSAVLMGGKNPNGAYWMVKNRGGFYTSAYYMNDYPVWVKEYNAQKPAD
                     KYFGMSWEMLLDKEKYPTMKPEILRLLDLPSSGFGHSFPHPLKGLTSDRPDTMYYKEL
                     IHTPFASEMVLDFAKAIVQNEALGSDEITDILCISLSANDEIGHSFGPSSPEVMDITV
                     QTDRLLARFFKYINTAVKEEDVLYVLTSDHGVPFFPEEMADRGVTAARVHPRKLAAVA
                     DSIMALKYGKLPLGKQYVDRIAYPDLYFDGRVLAEKKIDKEEASDYISAVFEQSLPII
                     YRAYSGTRLKRGLVPSDPISEAVRKSFHSRLSGDVVIVLKPFHVWDSNGLGTEHSLPY
                     AYDAQVPLIFKGKRWIKSGLYKDEVAPTDIVPVLSVITGCEDPGGRDGQVPDQLLKFS
                     K"
     gene            9031..9396
                     /locus_tag="PK937_13080"
     CDS             9031..9396
                     /locus_tag="PK937_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH33553.1"
                     /translation="MPGFLTTIKYVCIYASDFDRSLAFYRDGLGLQVTYKEDGFAQFN
                     TEGTILTLEKDGFRSDEPKDYRKVGFLIQFEVEDIEKAVTALKAKNVKFTQTITDKEF
                     GRTAIIVDPDGNQIMLLEQ"
     gene            9455..>10039
                     /locus_tag="PK937_13085"
     CDS             9455..>10039
                     /locus_tag="PK937_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNH33554.1"
                     /translation="MANITYLEAISMGLREEMQRDGNVFLIGEDIGVYGGAFKVTKGF
                     IDEFGAERVMDTPISEAGIIGVCVGSALMGMRPVAEMQFADFISCGFNQLVNQAAKIH
                     YRWGEAVPMVVRCPSGGGVHGGPFHSQNPEAWFFHVPGLKVVSPSTPYDAKGLIKAAI
                     RDNNPVLYFEHKFLYRRIKGEVPEDDYIVPIGKGD"
BASE COUNT         2689 a         2188 c         2406 g         2756 t
ORIGIN      
        1 gccaaattca tgccaacttc ttttattaaa tcataattaa ttcacacaac gggttattcg
       61 ttatacttta ccaatgatgg ttcctaccaa atggattata tgcgtaacgg gtttttgttc
      121 ccgattatca aatttacttc aaccccaaca ttgaaacgcg gcacgtcaaa aaatacatta
      181 cgcgtcatag ccaaaggttc gaaatttgat ctatatttta atgatcagtt tttagcagca
      241 tttgagcatg aggccatcga aaaaggcgga ttcggaattt ggtccggaca gggtagccat
      301 atatcggtgg acaatgtcaa aatctggaat attaaataaa ttttttattt caagtcctat
      361 caacagcagc catccggccc gcaacaggct tcgctcagaa tagtttcatt gtaatccata
      421 cctttagttt cgcgtgcgtg acggcgagca ttcttattgc aattaaagag ttttgccgat
      481 tctaaagaaa taatctcttt agggtttacc gcaaaaatat cattggcgta cggccctttt
      541 tcccgtgtat aaatgccgaa agttttatca cacacggcca tacgctcccc gcgaaaaagt
      601 gtgtgtccgt catcatctat aacggctttc cagggacctt tgtaaatcac ggcttgttgg
      661 cgttcaaggc aaggaccttc ttttcctttg taaccgatca cggtcactga acggtattcg
      721 atgccttcca ctacttgcca tggcgtggtt tcccatttgt ctaaactaat accgtagaac
      781 cccgcttttt caaagacttt caaaaattca tcttcgcgaa atgcaccgga tatacatccg
      841 ctccaaagtt caggattatt tttttgttga agcggtatgt cctcatcaga cacaatatcc
      901 gatatggcaa aacggccgcc cggttttaaa acgcgatata tttcggcgat gagattttga
      961 cggtcttctt cccgaacaag attgagcaca caattggaca aaacgacgtc aacggaatta
     1021 tcagaaatca tcggtcgttg gttgcgaagt tcttgagtaa acaactccaa acgttgcaaa
     1081 tcatcgccgt gttggatagg attttcacgc acataagcgt cgagcacgtc ccaatccaat
     1141 ttcaaatcct gtatttttcc gcgatgaaat gtaacattgg tataacccag tttttgtccg
     1201 ataggatctt tgtacttgcg ggctaaggct aacatttcgg aattcatatc cacgccgagc
     1261 acacgccctt cggaacctac gatttgcgcg gcgatataac atatttttcc gccgccgctt
     1321 cccaagtcca ataccgtttc tccggggcgc acataacgcg aggggtcgcc gcagccatag
     1381 tcacgatcaa ggatttcctg tggtaaaatt tgaagatatt ttggatcgta ggaaacagga
     1441 caacataaag cggcaacggt tgtttgcgca ccttcggtat atcgttcggt gacggccttt
     1501 tctacattga gtgcaaattc gtcgttagca gacatgctta actctccaaa agttattgaa
     1561 tcaaataata aatgcttgcc gcgatgacag cggcaacggg taatgtcagc gcccaggaaa
     1621 gaacgacttg tcgtatcact ttgaaatcgg cctgacgggt cacaatgcct attccggaaa
     1681 tagcgcctac ggatacatgg gtggtggata caggtaaacc ccaccgactt gcaaaaataa
     1741 caagaaatcc cgtaacaaga ttggctgtca gtccctgacc gtggttcatg gatgtgatgt
     1801 tttcgctcat ggtacgcgct actttgcggg cattgagtaa accgccgatg gccatagcta
     1861 ttgcgatggc tatcatcgac cactcgatac gtataatctg cgcggcaaat acgagcccaa
     1921 cgattttagg cgtatcgttg agtccgcgtg caaaactgac aatagccgcg ctgatataat
     1981 gtagcgcgtc caccatacgt tgtgccgaaa tgcccattac tgcgccaccg tatcgccttt
     2041 gacaggaagc ttcatcgccg aaagtaattt caggcagagt cactgactga aacgaaagag
     2101 cgtgcgctgg tcgaggggta ggtatgacgg tttggcatgt tccaacgcaa atgcaatgtt
     2161 cttctcgaat accagcccaa atccgaattt ggcgaaatac aacataaacg atcacggcaa
     2221 caaccacgga aaccaaaggg ctgactaata gaggtataaa aaaggctgag cctaattttt
     2281 cggtattcaa ttggggacct acagcaagga aaccagcgcc ggtaagcgca ccggtgagac
     2341 cgtgtgtcgt cgaaatggga aatccaaaaa atgtcgcgat gagaaccgtt aaaccggcgc
     2401 ccagggctac tgcagttaaa aattcggggg caaccgtcaa tgtatccggc acaagccctt
     2461 tgcccgaaaa attactcacg agtgattgag caataaaaat cgaacatacc gaacccgcaa
     2521 aggtggcgac ggtcgcgaac ccgatcgagg ctttatatcc taaggtttta ctgccgtaga
     2581 gcgttgcgac acctttaaaa ttatcatttg caccgttggt gtatgcgaca aaaaacgctc
     2641 cggcaaaaag cagtatgagc atcgccattt ttatcttcta attaatttca ggttatttat
     2701 tttgaattat gccgttgtcc caccgcaact gctgcccgac cccgccgtac acccgaaaca
     2761 gtgcgatgca aaaaggatag cgcgatttcg tattctggtc gcatcaaaat tgaaaacgct
     2821 aagcggttcg tgatcatgag attgcatgcc gagcatttgg ttgaaatcgc aatcgtagat
     2881 ttttccgtca taactcacgc tgatcagggt tcggcacatg acaccttgcg ccgcgccggg
     2941 attgaaagca tttattagct tcatcatata ttcgtcatat ttacccgtac gctcaagatc
     3001 ttcccgaaag cgatggatcg gcatattggt gatcgtaaat agattattga agtgaattcc
     3061 gaatttgtca tgcaaactgc gtttatagtc cgcctccaac gctccttgag cagcgggcag
     3121 gtatgtaccg acgggattgt acactaagtt gagtacaagg cctgaatcgt cttttccata
     3181 gcccagcgca ttgagatttt ttaacgccgc gatgctttta ttgaaaacgc cccgtccgcg
     3241 ttgcttatcc gtaaaatatt cctcataaaa aggtagcgat gaaatcactt caatcccgtg
     3301 atcggcgaaa aactgcggca ggtgcgtcat cggttgtttg gtcagaggat gagggtccag
     3361 cgatacggtg aggttatgcc gaaccatgac gtgttttccc aatcgtcgtg cctcggtaac
     3421 aaaaaactca aaatgcggat taagttccgg ggcgccgccg gtaatatcga ggttttttat
     3481 ttcgtcatgc gccgcaagta tcgctaaaca gtgttctacg gtaaggcgat ccatttgctc
     3541 ggtgcgcttg ggtgaagcgt ccacgtggca atgacggcag gcctgattgc acagtttggt
     3601 tatattgact tgcaacgtat gaatgccgag cggcggtaac gtcaactcat gctcacgcag
     3661 tttttcatga aaagaatatt tcatatcggc cggagatatc ggtaatattt gaggcagttg
     3721 cataatttct atacgttgag gttatctaaa ttattcagca tttgtacacc gtgtacgagc
     3781 gtgatgccgg ccatcatggc ggaggcgacg tgtaccgctt cggtcatttg gtcggggctt
     3841 gcgccgcatt cgaggctctg cgtcgtatag gcgtcaatac aataaggaca gtttttagca
     3901 tgggctacag ccaaagcgat cagggctttt tcccgtttgg ttagtgcgcc gtctttgccg
     3961 gtaacctcat tgtaatatgc aaaaaattta tccatcagtt ctttgcgaaa tctccctacg
     4021 tcgccaaatt ttttaaggtc ttcactttcg taataattca tcttgaggct cccatgttgt
     4081 aagaatatgg atgaagaaaa ggattaattt tatgaagact gaatgatgta actcggaggc
     4141 aaaacaaata ttcccatgtt tatttgttat ccacctgcga ttcgatgacg gattggattt
     4201 ctgcaggtac gcgattgagg aaatcatatc cggtggaggt ttcgatttga tccacggatg
     4261 ttcggtacga tttccatgac gtgctgcgaa tgccatcggt attgggcata tctacagcga
     4321 tcacgcgtgt cagcgtagtg acttgatcgg gtgtggtttc ctcgtgagga agtacaacaa
     4381 tgattttcca tacgcgtacg gggacggtga ctttgccgga agctatggtt ttgagagaat
     4441 cataatgccc tgacatgata aaaagttcgt aatcttcacc ggccaaatca cggcaataac
     4501 tttcgagttc gacccaaggc ccttggttat tatccgaagt ctgaggaagt atattggtca
     4561 tgtaaaatgt agatacatta tcggagggaa ctgcattgcg atcgccggag gggcacatgt
     4621 gtcctcgatc ataaccgctg ccggtgtagt cgccgtcttt cactgcatac caacccgacg
     4681 gcaaaacatc cgttttaaag ttatcttgac gtgctatcgt accgagccaa tttttactca
     4741 agtgccaagc gcaccagttg gggatgcggc gaatattgtc ataagacacc gcgtattgag
     4801 agcgagcgat cagatagtta cgcggtgaag taatatcagt ttcgctttga ctcggattgc
     4861 caagggaaag atgcaaattc ggcgtttggc cggtatcggc gtcagctatg gatagtcggt
     4921 atagttgtgt ctcgctgtag gcgccgtttt cactcaggat ttttatgaag tacgtgcctg
     4981 cgtttaagtg gggaatatca atggattctg tgatgccatt ggcgccacgg gataaaccta
     5041 caacggaatc gcggctatcc atgagataga gattataatc tttatcatcc ggaacggcca
     5101 aagtaaccgc gacatggcgc ggcgaactta atacaaaacg aaaataatct atatcgtcgc
     5161 tgacagaaac ggtgctttgc tgaacttggg gaagtctcgg aaataaaatc gccgtttcag
     5221 gcgtatcatt gccatcaatg gaatctaacg gctcgattaa atccgcacac cccgacccgg
     5281 ctaaaaatgc agcgatcaat acaaccggta acaagatttg gaaggatttc atataaaaag
     5341 ataaaaaaac ttatgattcg tccaaagaat ttttcaaagc ttttcttgtt tcttagtttc
     5401 aaaattatta tgatagcccg ccttaataat ccgctaatat tcacataaca aaatttacac
     5461 ttcgataatt caaatttaat aacgaggaaa cactatgagg gtagatcgta tcatcgaggc
     5521 gtttttacca cgtgaagaca aattttttaa actttttgaa gaaacagccc agcacctgat
     5581 cacggcatcg gatctgatgg tcaaactgat gaattgtccg gcagaggagc gtttagccgt
     5641 aagtcaggaa attcacaaat tagaacactt atgcgacgat acggcgcatc ggcttttttc
     5701 cgaactgaac gccacatttg taacgccgtt tgatcgcgaa gatattatga tgctggcttc
     5761 ggcgctggac gacgtcatgg attttttgga tgaaagcgca cgtcgcatca tattgtataa
     5821 aatcacttcc attccggcca atatgaaaca attggccgat gtgctcaaag aatctatcac
     5881 cgagacacat cacggcgtga gccatattcg gaatatccat aagacggagg gtattcgtaa
     5941 aactctggaa cgtatccacg cctgtgaaaa caaagcggat atggtttttg aaatgggaat
     6001 agcggacctt tttgataatg aaaaagaccc gatcaatttg gtcaaactga aagatattta
     6061 tacaagcctc gaacgctcca cggacaaatg cgaagatgtc gccaatgttt tggaagctat
     6121 cctcatcaag cacgcttaac cggagaattg cgatgacgga actgcttttc atcactatag
     6181 cgttagctct catttttgat tttatcaacg ggtttcatga tgcggcgaat tcgatagcca
     6241 ccgtagtttc gacgcgtgtg ctttcgccgt ttgtagccgt gctatgggct gcagctttta
     6301 attttctggc ttattggctt tttgaattgc atgtcgccga taccgtagct aagacggtaa
     6361 aacctgaagc tattacattg gttgtggttg catcaggact ggtcggtgcc atcatatgga
     6421 atttattaac ctggtggcta ggtattccat ccagttcatc gcatacgctg atgggcggtt
     6481 ttgcgggtgc ggcgatagcc tatagccatg gtgatttttc ggtcgtgcgt atggaaaaaa
     6541 tttcaccggt tttgtatttc atttttttag ctccggtcat agggatgata gtgtcttaca
     6601 ttatttcagt cattactatt catatctgca aacgtcagag tccgcataaa gtagataaaa
     6661 ttttccgccg cttacaatta ttgtcctctg cggctttcag cttaggtcac ggtggaaatg
     6721 atgcgcagaa agtgatgggt attattgcgg cagcgatgtt atctcagggt ataattgccg
     6781 acatgcatca tattccggaa tgggtacccc tggcatgtta tgggtccata gcgttgggaa
     6841 cgatgatggg gggatggcgt attgtaaaaa cgatgggaca aaaagtaact aaactttcgc
     6901 catttgaagg ttttgctgcg gaaacggccg gtgctgtgac attatttgga acaggatata
     6961 ccggtattcc cgtaagtacg acgcacacga ttaccggctc gatcatcggc gtcggcgtga
     7021 tcaaacgcgt atccgctgtt cgctggggcg taactcgcaa tctgctttgg gcatgggtat
     7081 taaccattcc gatttcaggg ttgtttggcg cgatttgtta ttggctcgct tcgttttttg
     7141 tttcataaaa cgtcggatta caattttttt attcgataaa aggttgctca taaatgagca
     7201 gccttttttt ttggatttct ttgtgctttt cttttttaat cgtgatatgt atcatgggtt
     7261 atgaatgaat cggggactac atgaatttga ggtatatatg aaaaacagaa gaaatatttc
     7321 atatttggct ttgataggta ttaccatttt cctttacggt tgttcatcca aatctcaacg
     7381 aacgaccccg cccaagccgc cgcgtcttat tgtttttatc gcattggatc agtttcgcta
     7441 tgactatctg acgcggttca gtacgcattt tggggcgaac ggatttaatt acctcctcaa
     7501 tggcggagca agttttgcca actgcttgta tgaacaagcc acgacgcaca catcggtggg
     7561 gcacgccacg atgatgagcg gtacgttgcc gtatcggcac ggtatcattg ccaactactg
     7621 gtggagtcgc gaactgcatc gctatatata tgcaccggaa gatacgctgg cgccgatact
     7681 gggaattgaa aaacaaaaag caacagatgg gcgttcgccg cgcaatttta gcggaatcaa
     7741 ttttgccgat gccttacgtg aaaaaacgaa cggccgggct aaagtgtttt ccgtgtccaa
     7801 taaggatcgc tccgccgtat tgatgggtgg gaaaaatccc aatggggcgt attggatggt
     7861 aaaaaaccgc ggagggtttt ataccagcgc gtattatatg aatgactacc cggtgtgggt
     7921 aaaggaatac aatgctcaaa aaccggcgga caaatatttt ggcatgtcgt gggaaatgct
     7981 gctggataaa gaaaaatatc caaccatgaa accggagata cttcgtttac tggatttgcc
     8041 gagttcaggt tttgggcata gtttcccgca tccgctcaaa ggattgacgt cggataggcc
     8101 tgatacgatg tattacaaag aactgatcca tacgccgttt gccagtgaaa tggttttgga
     8161 ttttgcaaag gcgattgttc aaaatgaagc tttaggttcg gacgagatta cagatatcct
     8221 ttgtatcagt ttatcggcca acgacgaaat agggcacagc ttcgggccat ccagtccgga
     8281 ggtgatggat attacggtac agaccgatcg cttgctggca cgttttttta agtatatcaa
     8341 tacggccgtc aaagaagaag acgttctgta tgtgttgaca tccgatcatg gggttccttt
     8401 tttcccggaa gaaatggccg accgaggtgt gacagccgca cgggttcatc cccgaaaatt
     8461 ggccgctgtg gccgattcga tcatggcttt gaagtatgga aaattgcctc tgggaaaaca
     8521 atacgttgat cggatagcgt acccggattt gtattttgac ggccgcgttt tggcggaaaa
     8581 aaagattgat aaagaagaag cgtccgatta tattagcgcg gtttttgaac aatcgctgcc
     8641 gatcatctat agagcgtatt cgggcacgcg gttaaagcgc ggtctagtac cgtccgatcc
     8701 tatatccgaa gctgtacgta agagcttcca ttctcgcctg agcggggatg tcgtgatagt
     8761 gctaaaacct tttcacgtat gggattccaa cggcctagga accgaacata gtcttccgta
     8821 tgcgtatgac gcgcaggtgc ctttgatatt caaaggaaaa cgctggatca aaagtggatt
     8881 gtacaaagac gaagtcgcac cgaccgatat agttcccgtt ttgtcggtga tcaccggttg
     8941 cgaagatccg ggcggtagag acggacaagt gcccgaccag ttattgaaat tttcaaaata
     9001 gttcatacat ctaaacagga gttcagttca atgccgggtt ttctcacaac aatcaaatat
     9061 gtgtgcatat acgcatcgga ttttgaccgt tcattggcat tttatcgtga cggcctcggc
     9121 ctccaggtaa catacaaaga agacggtttt gcacagttta ataccgaagg cacgatactc
     9181 acgctcgaaa aagacgggtt tcgttccgat gagcccaaag actaccgcaa agtgggtttc
     9241 ttaattcaat ttgaagtgga agatatcgaa aaagcggtta ctgcgcttaa agccaaaaat
     9301 gtgaaattca cccaaacgat tacggataaa gaattcggtc gtacggcgat catcgttgat
     9361 ccggacggca accagatcat gctgctggaa cagtaaacgg gcagatcgga ttcatttttt
     9421 ttgggtgcga atgtaaaatt tcacagaggt tctcatggct aatattacat atcttgaagc
     9481 catttcgatg ggtttgcgcg aagagatgca gcgcgacggt aatgtatttt tgatcggtga
     9541 agacatcggt gtctatggcg gcgcgttcaa agtgactaaa ggatttatcg acgaatttgg
     9601 cgccgagcgt gttatggata cgccgatttc cgaagccggt attatcggcg tgtgtgtagg
     9661 ctccgcactt atggggatgc gtcccgtggc ggaaatgcag tttgccgatt ttatttcctg
     9721 cggattcaat cagcttgtca atcaggccgc taaaattcac taccgatggg gcgaggctgt
     9781 accgatggtc gttcgttgtc cctcgggcgg cggcgtgcac ggcggccctt ttcactcaca
     9841 aaatcccgaa gcatggtttt ttcacgtacc gggattaaaa gtggtttccc cgtcaacgcc
     9901 ctacgatgcc aaagggctga tcaaagcagc gattcgtgat aataatcccg tattatattt
     9961 tgagcacaag tttttgtatc gccgcatcaa aggtgaagtg cctgaagacg attatatcgt
    10021 tccgatcgga aaaggggac
//
LOCUS       DAOQGF010000116         9781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3248_length_9781_cov_6.717150, whole genome shotgun sequence.
ACCESSION   DAOQGF010000116 DAOQGF010000000
VERSION     DAOQGF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_9781_cov_6.717150"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /gene="pruA"
                     /locus_tag="PK937_13090"
     CDS             complement(<1..1499)
                     /gene="pruA"
                     /locus_tag="PK937_13090"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNH33555.1"
                     /translation="MATARKKKTFKYSVFKNEPFTDFSKPANRKSFEKALEKVSKELG
                     KEYHIIIGGDRIRTNHKMESRNPSNREQLIGIFYDGNEELANKAVETAAEAFKKWSRV
                     APEKRASYLFKAASIMRKRKHEFSAMMVYEVGKSWAEADGDTAEAIDFMEFYGREMLR
                     YASEQPLTKIAGEDNQLMYIPLGVGAVIPPWNFPLAILVGMTTAAIVSGNTVVLKPSS
                     DSPTIAAKFVALMEEVGVPKGVINFLPGPGSGMGDAITKHALTRFIAFTGSKTVGLHI
                     NKVAAETQPGQLWIKRVIAEMGGKDSIIVDNEADLDKAVDGVLYSAFGFSGQKCSACS
                     RAIVVESVHKKFTEKLIAKAKTLTVGNPADPKNYMGPVINDKALGKMMWYIDKAKQEG
                     AELLLGGVKDDMQGSFLMPTIFDNVKPKDTISQEEIFGPVLAIIKAKNFDEALEIANN
                     TEYGLTGAIFTKSRKKLERARNEFHAGNLYLNRKCTGALVGVHPFGGFN"
     gene            1714..2577
                     /locus_tag="PK937_13095"
     CDS             1714..2577
                     /locus_tag="PK937_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33556.1"
                     /translation="MKIISESWFKRHRWTLLVFLFLVFRPHITAAIRNHVVIPFKKNI
                     LHDHRTMPSKKLFKPKAELRVHYAGIVLLNDTGDTEDDGCSDEDGDACSTESEYYETE
                     DDTEDDGCSDEEDDSCESSDEESDDSDYSDDDGCSDDEGCESDDDTYSSDDSDYYEDD
                     GCSCQGETVVYSDDTPENYYNSTLRVHFSVYTGGADWTIRIEDNYGKTLYTMMRVDNV
                     ATGNYSYNWSGDYYDDAIGQSRFILPGSYYVVLQRRDGGIYQSKKSFTYNSKNGFQVD
                     GLNINEINFMY"
     gene            complement(2567..3244)
                     /locus_tag="PK937_13100"
     CDS             complement(2567..3244)
                     /locus_tag="PK937_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNH33557.1"
                     /translation="MPSDKSGSVETIITDVEIIYIFDTLCGWCYGFGPVVQRLRENYS
                     REIPFTIMSGGLAVGDRVKPIGYMSHYILDVMPRLETMTGVTFGKPYRDLVEQGQFVL
                     DSLPPAVALAVCKKTKPEMAFDFASEVQSLHFMSGADTNQPDFYANVAKQFGLDGNKF
                     ILAINDPVWRETAQREFRDVHYLGIQGYPALVFRAGDQAYLIANGYTDFDALDLRLKS
                     ILRTVNT"
     gene            complement(3368..4579)
                     /locus_tag="PK937_13105"
     CDS             complement(3368..4579)
                     /locus_tag="PK937_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33558.1"
                     /translation="MGFSNIFKIAFNALMRNKMRSALTMLGIVIGVGSIIAMTGVGAG
                     AQKEIDNQTAAFGTNMITIRSGGMTAGGIRIRAMKELQPSDGDAIVRGSELVTAQTPV
                     IRLVTNVVSSERNWASQVYGVNTSFTTIRNWGMMHGAMFTDQDYRSGNKVCVLGSTVA
                     VNLFGQSDPTGEIVRIQNIPFRVAGVLESKGQLASGQDQDDMIIIPYTTYDKRLTGDK
                     NIDYIYVSVRTSQDMTTAMEEIRQILRQEHRIPAYGDDDFTIRTQEQVNEIAKSISKT
                     VSLLMIIVASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGATEKHILLQFLVEAVV
                     LSLTGGVIGMILGVVIGYLVETFTSWAVSISMVSVVVSMSFAAGVGIFFGFYPARKAA
                     SLNPIEALRFE"
     gene            complement(4591..5391)
                     /locus_tag="PK937_13110"
     CDS             complement(4591..5391)
                     /locus_tag="PK937_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH33559.1"
                     /translation="MSQPTQQIFHPLMERAGSKPLLSVRNIKKTYRMGDIEVMALRGV
                     SLDIYEGEFVAIMGSSGSGKSTFMNTLGCLDQPTSGEYLLDGVSTGHMTKTDLARIRS
                     FKIGFVFQGFNLLSRTSAIENVELPMLYQGVAPKERHDRAMEALKAVGLEKRYDHKPN
                     ELSGGQQQRVAIARALAAHPSMILADEPTGNLDSKTSIEIMQIFMRLNDQGITIVLVT
                     HENDIAQYCKRNVVFRDGRIIKDFMVERRLEPEEEMRKVLATSIVDNA"
     gene            complement(5405..6562)
                     /locus_tag="PK937_13115"
     CDS             complement(5405..6562)
                     /locus_tag="PK937_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH33560.1"
                     /translation="MNKKIWIIGGIAVLVAVILGWIVWGGSTAAGGYKTEAVTRGDIS
                     VVITATGKVNPVTRVQVGTQITGTISRIFADFNQKVRKGQEIAEIDPTFLKAQLLEAE
                     ANAEKAKAQVEQSKKTLDRATELFDRKLISQSEKDEALTNYNLAGAQYKQAMAAYNRA
                     EVSLRYTSIVSPIDGVVISRNVDVGQTVAASLQAPILFVIANDLSKIQVEATIDEVDI
                     GKVKVNQEVSFYVDAYPDEKFKGRVAQVRLQPITTQNLVSYEVIIEVENRDNKLLPGM
                     TANLSIIVDTKKDVIKVPNMALRFQPALTQEQFKKYSAMYGDKFDLKNSAMVWTLQSG
                     ELAPIIVDPGLTDGQYTEIRSDQLTIEQILVTGISTVAAPGSARDRFAPKK"
     gene            complement(6582..7928)
                     /locus_tag="PK937_13120"
     CDS             complement(6582..7928)
                     /locus_tag="PK937_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH33561.1"
                     /translation="MKFIFVWVILAARSGWSQEVITISTEDTLTLDRCIEIALKNQPA
                     LGAAQGAIITAESDYTQVRSLRFPQIQMEAGAYVTNTPLGSSNAIAQAPVIEPKNNTD
                     FIPTIRLTVKQPIYDFGRTDKSLESKSKLIRAAEMSLQATEDDVILNVHTSYYNYVLA
                     QQILKINEERVTQSRKHLERARGFFAVGKIPESEVYKAELEVSNAELELTNARGKLQL
                     AKVSLNSAMGVADWTDDVTSYKVASHENYVAFNANLRESINSAFTGRREVAASEMRVQ
                     AWRSALSAAKSQYYPIITASGGFGPYIVRNTLTTGETKDKFKIGYNIGVNFEFPIFQG
                     LNVRADISEAQGGIRIASSQYNTTKQRIAQEVQERYYSVKFAEERYKSTERIVTQTER
                     NLNLAEGRFDTGVGSAIEVTDANFSVANARIERTTALYNYKIELARFQRTIGTLRK"
     gene            complement(8085..8864)
                     /locus_tag="PK937_13125"
     CDS             complement(8085..8864)
                     /locus_tag="PK937_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNH33562.1"
                     /translation="MLFDTHAHLDYDLFDDDREAMIARAREAGVDTIVTIGTNLQSAE
                     RAKTIAEKYDFVFFAVAHHPADAHTYQSSMMPHYEKLIQHPKCVAVGETGLDYYWKEA
                     APEQQLPLFRSFIRMACVYDKPIIIHNREANHDIISVLQEEKQNGKLSNLRGIMHCFS
                     GDAEAVKQALDLNFYISFSGNITYKKSHLPSIIPMIPHDRILIETDAPFLTPVPHRGK
                     RNESANLRFTAEKLSEINKWTLGETESITSANARRIFGMTH"
     gene            complement(8869..>9781)
                     /locus_tag="PK937_13130"
     CDS             complement(8869..>9781)
                     /locus_tag="PK937_13130"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNH33563.1"
                     /translation="DPRKAKLATASAPAKKGEKHSIDFYKNLDLDINIDLPENADNAI
                     RSAEMLLTLYGNIGVTKPPESEEFFLSGDVKTRPGGKYAYLSIAFNIEKGDIVFTGEP
                     GVNPNLDIVAKKRFEYKDSEGQSIPAEARIQVTGKLLKPEVAISAVERGTEDSPLPDL
                     TEPADILSYLVLGVKTKDLAKVDASQAKDIVQQTAINQVLNIVANKAGLSKLEYIPST
                     SDASKATIEVGKRISETVSVNFSGGTDPTVGRSITLEVVADSVWGVRKFLKSWKKTLE
                     FEYKQPPQEKTDQPDIFNLLLYFRKEY"
BASE COUNT         2601 a         2342 c         2029 g         2809 t
ORIGIN      
        1 atgttgaacc cgccgaacgg atgcacgccg accaatgctc cggtacattt acgattcaag
       61 tacagatttc ctgcatgaaa ttcattgcgt gcacgttcca attttttacg acttttcgta
      121 aaaatagcgc cggtcaaccc atattccgta ttgttcgcaa tttctaacgc ttcatcaaaa
      181 tttttggctt taatgatggc cagcaccggg ccaaaaattt cttcctgcga aatcgtatct
      241 ttgggtttca cattatcaaa aattgtcggc atcaaaaagc ttccctgcat gtcatctttc
      301 acgccgccca gcagaagttc agcgccttct tgtttggctt tatcaatata ccacatcatt
      361 tttccgagcg ctttatcatt gatcaccggg cccatatagt ttttaggatc agccggattg
      421 ccgacggtca atgttttagc tttggcgatc aatttttcag tgaatttttt atgaacgctc
      481 tcgacgacaa tcgcccggga acatgcggag cacttttgac cggagaatcc aaacgccgaa
      541 tacaacacac cgtccacggc tttatccaaa tccgcctcat tgtcaacgat gatcgaatct
      601 ttgcccccca tctccgcgat cacgcgcttg atccagagtt gaccgggttg tgtttctgcg
      661 gcgactttat tgatatgcaa acccacggtc tttgagccgg tgaatgcgat aaaacgtgtc
      721 aatgcatgtt tagtgatggc atcacccata ccggaaccgg gtcccggaag aaaattgata
      781 acccctttcg gtactccgac ttcttccatc aacgcaacaa attttgctgc aatcgtcggt
      841 gaatcactcg acggcttgag tacaactgta ttaccggaaa caatggcggc cgttgtcata
      901 ccgacaagaa tcgctagcgg aaaattccac ggcggtatca cggcgccgac acccaaagga
      961 atatacatca gttggttatc ttcgccggct attttcgtca atggttgctc cgacgcataa
     1021 cgcagcattt cgcgcccgta aaattccata aagtcaatag cttcggccgt atcgccatcg
     1081 gcttcggccc aactcttccc gacttcataa accatcattg ccgaaaattc gtgtttacgc
     1141 ttacgcataa ttgatgcggc cttgaacaaa taagatgccc gtttttcggg agcgacgcgg
     1201 ctccattttt taaaagcttc agccgccgtt tcaacggctt tattagctaa ttcttcattg
     1261 ccgtcataaa aaataccaat gagctgctca cgattggacg gattacgcga ctccattttg
     1321 tgattggtac gaatacggtc accaccgata atgatatggt attctttgcc taattcttta
     1381 gaaacctttt ccaaggcttt ttcaaatgat ttacggttcg caggtttcga aaaatccgta
     1441 aacggttcat ttttaaatac gctgtatttg aaggttttct ttttacgtgc cgtagccata
     1501 cgtttgctcc tgcattcgat ctaagtgatt tttttgaatc aaaattactt ttcgaatata
     1561 gcgaatatga taaaaatcta caagaaaata gcgtatgcga taagatacac attcactttt
     1621 ttcacactca ctaaagctcg actgcattgt aaatacaagc tttaagaatg gcacaaggtt
     1681 tgaatgttat cggttatcaa accggagttg tgtatgaaaa tcatatctga atcatggttc
     1741 aaacggcatc gctggacttt attggttttt ctatttttag tttttcgacc gcacattaca
     1801 gccgctattc gcaatcatgt agttattcca ttcaaaaaaa atattctgca cgatcaccgc
     1861 accatgcctt ctaaaaaatt attcaaaccc aaagccgaat taagggtaca ttatgccggg
     1921 atagtgttgc tgaatgatac gggcgacacg gaagatgacg gatgttccga tgaagatgga
     1981 gatgcctgtt cgactgagtc cgaatactac gagaccgagg acgatacaga ggatgatggc
     2041 tgcagtgatg aagaagacga tagctgcgaa tcttctgatg aggaatccga tgacagcgat
     2101 tattcggatg atgacggatg ttccgatgac gaagggtgcg aatccgacga cgatacatac
     2161 tcttcggatg attccgatta ttatgaagac gatggatgct cctgccaagg tgaaactgtg
     2221 gtatactccg atgatacgcc tgagaattat tataatagca ctctgcgcgt acatttctca
     2281 gtctatacag ggggcgcgga ttggacgatt cgcatcgaag ataactacgg gaaaacgttg
     2341 tatacgatga tgcgagtaga taatgtggcg accggaaatt attcgtacaa ctggagcggt
     2401 gattattacg atgacgccat cggacaaagt cgttttatcc tgccgggaag ttattacgtc
     2461 gtactccagc gaagggacgg cggtatctac caaagtaaaa aatcattcac ctataattca
     2521 aaaaacggat ttcaggtgga cggcctgaat atcaatgaaa tcaactttat gtattaactg
     2581 ttcgcagtat gcttttaagc cgaagatcaa gcgcatcaaa atcggtatat ccattggcta
     2641 tcagataagc ttgatctccg gcacgaaata ccagtgccgg atacccttga atacctaaat
     2701 aatgtacgtc cctaaattct cgttgcgcgg tctcacgcca caccggatca ttaatcgcaa
     2761 gaataaattt atttccgtct aatccgaatt gttttgcaac gtttgcataa aaatccggtt
     2821 gattggtatc ggctccgctc ataaaatgaa gtgattgaac ctcggacgca aaatcaaatg
     2881 ccatttcagg ctttgtcttt ttacatacgg ccagcgctac tgcaggaggc aacgaatcca
     2941 atacaaactg tccttgttca accaaatcac gatacggttt cccgaatgta acacccgtca
     3001 tagtctctaa cctcggcatc acgtctaaaa tataatgcga catgtagccg ataggcttca
     3061 ctctatcgcc cacagccaat cctccgctca tgatcgtgaa cggtatttcc ctggaatagt
     3121 tttcccttag tcgttgaacg accgggccga aaccgtagca ccaaccacac agcgtatcaa
     3181 aaatataaat gatttccaca tctgtgatga tcgtttctac tgaaccggat ttgtctgaag
     3241 gcatagaatt gcaccaatgg tattgttata aaaaaaacca acgctttcgc gatggtttgt
     3301 tatttcaaaa taaattggag atcgaacact atttacatat atgccgcagt tttgagaaaa
     3361 ttaatactta ctcgaaacgc aatgcctcta tcgggtttaa cgacgcggct tttcgggcag
     3421 gataaaaacc aaagaaaatg ccgacaccgg cggcgaaaga cattgaaaca actacggaaa
     3481 ccatcgaaat actgacggcc caactcgtaa atgtctcaac caaataaccg atcacgacac
     3541 ccaatatcat gccgatcacg cctccggtaa gactcagtac tacggcttcg acaagaaact
     3601 gtaacaaaat gtgtttttcc gttgcaccga tcgccatacg aatgccgatt tcacgtgttc
     3661 gttccgttac cgaaaccaac atgatattca taatgcctat accgcctacc aatagtgaaa
     3721 tcgacgctac gataatcatg agtagcgata cggttttgga aatagacttg gcaatttcat
     3781 tgacttgttc ctgtgtgcgt atcgtaaaat catcatcgcc gtatgcagga atacgatgct
     3841 cctgacgcaa aatttgacga atttcctcca tcgctgtagt catgtcctga cttgttcgca
     3901 cggaaacata gatataatca atatttttgt cgcccgtcag tcgtttatca taagtcgtat
     3961 atggaatgat gatcatatcg tcctgatctt ggccggacgc caattgacct ttcgattcga
     4021 gtacaccggc aacacgaaac gggatattct ggatacgaac gatttcccct gtcggatcgc
     4081 tttgcccgaa aagattgact gccacggttg agccaagtac acagactttg tttccggaac
     4141 gataatcctg atccgtaaac atcgcgccat gcatcatacc ccaattgcgt atggtcgtaa
     4201 aacttgtatt gacgccatat acttgagatg cccagttacg ttccgatgaa acgacattag
     4261 ttacaagacg aatcaccggc gtttgtgccg tgactaattc cgacccgcga acaatagcgt
     4321 cgccatcact gggttgtaac tccttcatcg cacgtattcg gataccgccc gctgtcatac
     4381 cgccggaacg aatcgtaatc atattggtac caaaggcggc cgtctgatta tcaatttctt
     4441 tttgcgcacc ggcacccaca ccggtcatgg caataataga accgacgccg atcacaatcc
     4501 ccagcattgt aagcgccgaa cgcattttat tacgcataag cgcattaaat gcaattttga
     4561 atatatttga aaagcccata tcaattcctg ttaagcatta tcaacgatgg aagttgcaag
     4621 taccttacgc atttcctctt cgggttcgag acgtcgttcg accataaaat ccttaataat
     4681 acggccgtca cgaaaaacaa cgttgcgttt acaatattgt gcaatatcgt tttcatgtgt
     4741 cacgagtacg atcgtaatac cttgatcgtt gaggcgcata aaaatctgca taatttcgat
     4801 actggtttta ctgtccaggt ttccggttgg ttcgtcggca agaatcatag atggatgtgc
     4861 cgccagagcg cgcgcaatcg caacgcgttg ttgttgaccg ccggaaagtt cattgggttt
     4921 gtgatcataa cgtttttcca agcctaccgc tttaagcgct tccatggcgc gatcgtgccg
     4981 ttctttggga gctacgcctt gatagagcat cggcaattcc acgttttcga tagcactcgt
     5041 acgcgacaat agattgaatc cctgaaaaac aaatccgatt ttaaaactac gtatgcgcgc
     5101 cagatcggtt ttggtcatat gacctgtgga tacgccgtct agaaggtatt ccccggatgt
     5161 cggttggtca agacatccaa gcgtattcat aaacgttgat ttgcccgatc cggaagatcc
     5221 catgatcgca acaaattcac cttcgtaaat atccagcgat accccgcgta aggccatcac
     5281 ctcgatgtca cccatccggt atgttttttt gatgttgcga acggataaca atggtttgga
     5341 tccggcacgc tccatcaaag gatgaaatat ttgttgtgta ggttgactca tatttaatga
     5401 tgatttattt ctttggtgca aagcgatcgc gagcactacc tggcgccgca acagtgctga
     5461 tgccggtcac caatatctgt tctatcgtaa gttgatcgct acgaatttcg gtatactggc
     5521 cgtccgttaa ccccggatcg acgatgatcg gcgccagttc gccggattgt aatgtccaaa
     5581 ccatcgcgct gttttttaga tcaaatttat caccgtacat tgccgaatat ttcttgaatt
     5641 gctcctgcgt cagcgccggc tgaaatcgca atgccatatt tggaaccttt atcacatctt
     5701 ttttggtatc aacgatgata gacaaattgg ccgtcatgcc cggcaacaat ttattatcac
     5761 gattctctac ttcgatgatt acttcgtagc taaccaagtt ctgtgtagta atcggctgca
     5821 aacgaacctg agccacacga cccttaaatt tttcatccgg atacgcatct acataaaaag
     5881 acacttcttg attcactttt actttgccga tatccacttc atcgatcgtt gcttcgacct
     5941 gaattttgct gagatcgttg gcaatgacaa aaagaatcgg cgcctgaagg cttgcggcga
     6001 cggtttgtcc gacatccaca ttgcgcgata tgaccacacc gtctatcgga gagacgatgg
     6061 atgtataccg gagcgatacc tccgcacgat tataggcggc catagcttgt ttgtattgcg
     6121 ctccagccaa attgtaatta gtcagcgcct catctttttc cgattgggaa atcaattttc
     6181 gatcaaaaag ttcggtagct cgatcgagcg tttttttcga ttgttctact tgcgctttgg
     6241 ccttttccgc attggcttcg gcttctaaaa gttgtgcttt taaaaatgta ggatcaattt
     6301 cggcaatctc ctgccctttg cgcaccttct gattaaaatc ggcaaagatg cgtgaaatcg
     6361 tgcccgtgat ctgagtaccg acttgcacac gcgtgaccgg attgactttg ccggtcgccg
     6421 tgatgaccac ggaaatatcg ccgcgcgtaa cagcttccgt tttataaccc ccggcagcgg
     6481 ttgatccgcc ccacacaatc catccgagga ttacggccac aagcacggca atacctccga
     6541 tgatccatat tttcttattc atgtatagct cctaaaaaca attattttcg caaagttccg
     6601 attgtgcgct gaaaacgcgc cagttcgatt ttgtaattat ataatgccgt tgtacgttct
     6661 atgcgcgcat tggctactga aaaattggca tccgtcactt cgatggctga accgacaccg
     6721 gtgtcaaatc gcccctccgc cagattgaga ttacgctccg tttgagtaac tatacgctcg
     6781 gttgatttgt atcgttcttc cgcaaacttc acactgtaat atctttcctg cacttcctga
     6841 gcaatacgtt gtttcgtagt attgtactgc gaacttgcaa tacgaatccc gccttgcgct
     6901 tcggatatat cggcacgtac attgaggcct tgaaaaatcg gaaactcaaa attcacgccg
     6961 atgttataac cgatcttgaa cttgtctttt gtttccccgg tagtcaatgt gttacgcacg
     7021 atatatgggc caaaaccacc cgaagctgtg ataatgggat aatattggct tttggctgca
     7081 ctcaatgcag aacgccatgc ttgaactcgc atttccgaag ccgctacctc acgacgcccg
     7141 gtaaacgcag aattgataga ttctctgaga ttcgcgttaa aagctacata attttcatga
     7201 gacgccactt tataacttgt tacgtcgtcc gtccaatcgg ccacgcccat agcgctattg
     7261 aggcttactt ttgctaattg taattttcct cgcgcattcg tcaattcaag ttcagcgtta
     7321 gaaacttcaa gctccgcttt ataaacttcc gactctggaa tttttcctac ggcaaaaaaa
     7381 ccacgcgcac gctctaagtg ttttcgcgat tgcgttactc gctcctcatt gatttttaaa
     7441 atttgttgcg caagcacgta gttgtaataa ctcgtatgaa cattgagtat cacatcatct
     7501 tcggtagctt gcagagacat ctcagcagcg cgaattaatt tgcttttgga ttcgagcgat
     7561 ttatccgtac gaccaaaatc ataaatcggt tgtttgacgg taagtcgtat ggtgggaata
     7621 aaatccgtgt tgtttttagg ttcgatcacc ggtgcctgag ctattgcatt ggaagaccca
     7681 agaggtgtat tggtcacata agcccctgct tccatttgaa tctgcggaaa acgaagtgag
     7741 cgcacctgcg tataatcgga ttcggccgtg ataatagcac cttgcgctgc accgagagcc
     7801 ggttgatttt tgagagcaat ctcaatacag cgatcaagcg taagcgtgtc ctctgtcgaa
     7861 atggtaataa cttcttgcga ccatccgctc cgcgcggcca atattaccca cacaaaaata
     7921 aatttcatac aacgcatgtt ttttcctttg agtaacgatt cagtctttga tctacaaacc
     7981 ccatgccaaa cgttattttg ataaacccat attttacacc gtgaaaatac taatattaat
     8041 ttctattgca atacttcaca tgcttaattt tcacagtgtg aaattcagtg ggtcattccg
     8101 aaaatgcgtc gggcgttagc tgaagtgatc gactcggttt cacccaatgt ccatttgttt
     8161 atttcactta acttttcggc cgtaaaacgt aaatttgccg actcattacg ttttccgcgg
     8221 tgcggaaccg gtgtcaaaaa tggcgcatcc gtttcgatca aaatacgatc gtgcggtatc
     8281 ataggaatga tggaaggcag atggcttttt ttataggtaa tgttccctga aaaagagata
     8341 taaaaattca aatccagcgc ttgttttaca gcctctgcat caccggaaaa gcaatgcatt
     8401 attcctctta aattactcaa tttcccgttt tgcttctcct cctgcagtac gctgatgata
     8461 tcgtgattgg cttctctgtt atgaataatg atcggcttgt cataaacaca cgccatcctg
     8521 ataaaagaac ggaatagggg gagttgttgt tccggtgccg cttccttcca ataatagtca
     8581 agccccgttt cgccgacagc cacacacttg gggtgttgta tcagcttttc atagtgcggc
     8641 atcatagatg attggtatgt atgagcatcg gcaggatgat gcgctacggc aaaaaaaacg
     8701 aagtcatact tttcagcaat cgttttagct cgttcagccg attgtaaatt ggtaccgata
     8761 gtcacgatcg tatctactcc cgcttcgcgc gcacgtgcga tcatcgcttc gcggtcatcg
     8821 tcaaacaaat cgtaatccag atgcgcatgc gtatcaaaca acatagaatc agtattcttt
     8881 ccggaagtag agcagaaggt taaaaatgtc gggttgatcc gttttttcct gcggtggttg
     8941 cttatattca aattcgagtg tttttttcca ggattttaaa aatttgcgaa caccccacac
     9001 gctatcggcc acaacttcca atgttatgga tcgtccgaca gtagggtccg taccgcctga
     9061 aaaatttacc gaaactgttt cactgatgcg tttaccgacc tcgatcgttg ccttgctcgc
     9121 atccgacgtg ctgggtatgt attccaattt tgataatccg gccttgttgg ctacaatatt
     9181 caacacttga ttgatcgctg tttgctgcac aatatctttt gcttgcgacg catccacctt
     9241 ggccaaatct ttagtcttta caccgagtac caggtaagac aaaatatccg caggctctgt
     9301 caaatccggc aatggtgaat cttccgttcc gcgttcgacg gccgaaatgg ccacttccgg
     9361 tttgagtaat ttaccggtta cctgaatgcg tgcttcggca ggaatacttt gaccttccga
     9421 atctttgtat tcaaatcgct tcttagcgac gatatccaaa tttggattga ctccgggttc
     9481 acccgtaaat acgatgtcgc ctttttcgat attaaacgca atactcagat aagcatattt
     9541 cccacccgga cgcgttttca cgtcaccgct taggaaaaat tcttcggatt ccggtggttt
     9601 ggttacaccg atattcccat agagcgtaag taacatttcc gcgcttcgaa tcgcattatc
     9661 cgcattttcc ggtaaatcaa tattgatatc taaatctaga tttttgtaaa aatcaatgga
     9721 gtgcttctcg ccttttttgg ctggtgctga cgccgtggct aacttggctt tacgagggtc
     9781 c
//
LOCUS       DAOQGF010000117         9726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3270_length_9726_cov_7.037949, whole genome shotgun sequence.
ACCESSION   DAOQGF010000117 DAOQGF010000000
VERSION     DAOQGF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_9726_cov_7.037949"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2481)
                     /locus_tag="PK937_13135"
     CDS             complement(<1..2481)
                     /locus_tag="PK937_13135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNH33564.1"
                     /translation="MNTRFDRSTFIQKFVLETRENIRRLNNALLRIEKNPGDTDAPDE
                     ILRTLHTIKGSARMLNLRAISELMHAFEEAFGNAHKQKYMHERAVMDTFLRTLDKTDT
                     HLDDIVRDGHSEADLSSWLHRITLIAERKINELQKEASADTSDKKITETTEPVAEPVT
                     PKKSSKTVTLDRGLFIKRFLTEAEEHVRNLKQHTAAWQKQHDAASLEIAIRAAHTLKG
                     SARMMKFENVGNISQRAEFFFRGLKEKKLTWSAGADQVLVMAYQSLDDLIEKIRITEK
                     DEFQNAALMDMLDEINAGAVPTEEAVRDLIRKPAHPVGETRPVREVKSGASGPDRLGE
                     RLIEAGLITREELMHANQTTDTRMPLGERLVALGYINRDQLNQLLMEQKSSRELLGQT
                     QINAGRITQKEEVLNSDQFVHVRIDKLDELIKSVGDISTKQIKTEEHIAVLRKIYLAL
                     KYWNSELRDYENAAQAGNVIDNPHLSQRLSETADELADVIKIAREEAANFDLSLNAIQ
                     TGIMGMRMVPIRSIFDGFPRAVRDLAKNLGKDVKLVVEGAETELDRKIVESLNEPLIH
                     LIRNAIDHGLEPSPDRIAAGKTAEGLLRIAAYNEGNTILIEVEDDGRGIDLERIKAKA
                     IEKKIVTDEQDISRFSENDLINLIFLPAFSTSELITDISGRGYGMDVVKRAIESIKGY
                     IEVVTAPNVGTRFSIHLPLTLTSLRALFVKSAAYKFAVPMQAVAETLKIKHDDIIEVV
                     EKKAIRLRNQLIPIVRLNEVVGYAASDEERDERFLIIIHANGEKAGFVVDDIIDEREI
                     LVKSLPKQFAKVKHISSATVASNNEIIMV"
     gene            complement(2478..3941)
                     /locus_tag="PK937_13140"
     CDS             complement(2478..3941)
                     /locus_tag="PK937_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNH33565.1"
                     /translation="MQQEFTRALFETFRKTILDFCGIAIPDDSRSELERKIRERMTQT
                     GFSDFYSYHVHIAGREAGEDELRKFIESITNNETYFFREPAHFRVLKSHIVPELLRKK
                     KERRIRVWSAGCSTGEEAYTIAISLFETRMLLGDFEIEVLATDIDRNALTIAQLGNFG
                     RNSFRAIDPSYLNTYFRIPDQRLMDYRIIDERIRAITRFEYLNLFDDHYPDYLHDIDV
                     IFFRNVSIYFPKDKIQSVNRRLSEVLNESGYLFLGSSETLHHNFGHLDLVDYDGVFLY
                     QKKTGTEEKSKKTIAPSLHESKRHHRPVSPKESAKPQTPRSASSRTKPEAPPSLDQVF
                     AMYKDGAYAQILALWDMHADGLEPKIPFLILKAFIYIGQEDFDLARKTCDAMMALDAL
                     NAEVYFLSGLVDYHAGQFAAATEKLKKALFLKKDLAMAHYYLASVYRQSGKTDDARRE
                     FRNTIKILEHKQDTSVSFLAQGYSSDYLINACKQYLK"
     gene            complement(3951..4979)
                     /gene="cheB"
                     /locus_tag="PK937_13145"
     CDS             complement(3951..4979)
                     /gene="cheB"
                     /locus_tag="PK937_13145"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNH33566.1"
                     /translation="MSQIRVLIADDSPTAVDIIATILETDDDIRIVGKAVNGREAVAM
                     AETLRPDVITVDIQMPIMNGLDAIKQIMAHYPTPILVITSSTDASIAFQALSFGALEV
                     VEKPSLELDFHARKYEEFIRKVKLIARVKVITHISGKMVDVKTVSDTSDFLLVIGIVA
                     STGGPRALASILGSLPKNLSAPVLVVQHIADGFTDGLIQWLADVSPLRVKSAEQAEAL
                     ARGTVYIAPNGKHLEIGAQNKIVLSEKEDVSGHKPSGSVLLRSLATHAKRHAVGVLLT
                     GMGRDGADGMLAIKQNGGHTIVQDEATSLIFGMPKAAIDIGAAEIVLPAERIAEQITR
                     WTGIKTDA"
     gene            5151..6236
                     /locus_tag="PK937_13150"
     CDS             5151..6236
                     /locus_tag="PK937_13150"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNH33567.1"
                     /translation="MLRIPIVRYYSLHLHTHAPHDTVPYPDLYASFAQTAYSQMILSD
                     VAFDLKTMQKQTRNTALDEFQVIENYTAKLDDHSTINITIYEAYDLPKDLLIADLSEE
                     LLETLEIIYLFYKGAIFPGIHKLEMLLDMGVIAMLPFREILSRVLPDMLKINILAEYQ
                     LMNFDKHDDDFIDFNLIWFLGKLNLQTVDKFLSDNDWHHDTPDFNNRDEESPPFGDAY
                     DPAESESNDADASAGAFDLNEEDRLLKTELENELKHFYQDQSHFYTRVVGLRHTEFDI
                     STLSEGARLTLVPEPENTHDPMAVSVHSARGEKIGYLKKELAALLFSELRQGVVFHAT
                     VVKVLPEAEEANLRLHILVRKWTHHFN"
     gene            6265..7182
                     /gene="glsA"
                     /locus_tag="PK937_13155"
     CDS             6265..7182
                     /gene="glsA"
                     /locus_tag="PK937_13155"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HNH33568.1"
                     /translation="MDISTLNHAVLAAKPHLQNGRVADYIPALREADAQHLGVAVADT
                     GGAVIGAGDCQVSFSIQSVSKIFSLACVLRQRGLDAVLQHVGMEPSGNPFYSLVQLEY
                     ESGKPRNPFINAGAIAVTSLLPGQTAGEKSEFLCRFLSEISGTPIAVNEKVYFSEYET
                     SHRNRAVAHFMKHFGNLEGNVEEAVNAYFQQCSITMDAVTLARLGLFLAHHGTDPVSG
                     KTIVDAKSQRFLLAMMTMCGLYDASGEFAVRVGLPAKSGVGGGIFTVVPGRYTIVTFG
                     PALDDKGNSIGGIQILEHLSSACQLSSWV"
     gene            7733..8479
                     /locus_tag="PK937_13160"
     CDS             7733..8479
                     /locus_tag="PK937_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNH33569.1"
                     /translation="MNIIVCMKQVPDSETRVKIGADGKSVDLSAANFVVNPYDEFAIE
                     EGIRLKEKFGGEVTILTLGTDRAENDIRKGLAMGADKAILLKADAFDGDVAHALAEEI
                     KAGAYDLVLFGKQAIDEDSSVMPQMIAEKLNLPCVTVVTKLDVQAETKAITCEREIEG
                     GKEIVELSYPAVIGTQKGINEPRLPNLKGIMAAKKKTIDKKDPAKAPAMTEIVAIELP
                     PARPAGKIVGKGADAVPELLRLLHEEAKVI"
     gene            8524..9492
                     /locus_tag="PK937_13165"
     CDS             8524..9492
                     /locus_tag="PK937_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNH33570.1"
                     /translation="MSVIAFAEQRGGAFKKSAFEAVAEAKRLADKLGTQAVAVVIGNN
                     VSGIAAELGKYGASKVYVAENAALENYSTEGYTQALNEAVKKENAAVVLLGATAMGKD
                     LAPRTAARLGAGIASDVTGLDVEGGKITITRPIMSGKAFQKLRITTATQVITIRPNVF
                     KATESGATAAVENLSMAIAPIRATVKNTVVESGKKVELTEADVVVSGGRGLKAPENWN
                     LVVELAEAFGGAHGASRAVVDAGWRSHEEQVGQTGKTVSPQLYIACGISGAIQHLAGM
                     SSSKYIVAINSDAEAPIFKIADYGIVGDAMEILPAMTAHVRKIKGK"
     gene            9644..9721
                     /locus_tag="PK937_13170"
     tRNA            9644..9721
                     /locus_tag="PK937_13170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9679..9681,aa:Val,seq:cac)
BASE COUNT         2387 a         2482 c         2384 g         2473 t
ORIGIN      
        1 gaccatgatg atttcgttat tggaagcaac ggtcgccgac gatatatgtt tgaccttggc
       61 aaattgtttc ggcaatgact ttacgagaat ctcccgttca tcaatgatat catccaccac
      121 aaatccggct ttttcgccat tggcatgaat aataatcaga aaacgttcgt cgcgctcttc
      181 atcggaagcg gcataaccga cgacttcatt caaacgcacg atgggaatga gctgattgcg
      241 gaggcgaatc gcttttttct ccacgacttc gatgatatcg tcgtgtttga ttttgagtgt
      301 ctcggccacc gcctgcatcg gcacggcaaa tttataagcc gccgatttta caaacaacgc
      361 tctcagggag gtcagcgtca acggcagatg gatcgaaaat cgcgtgccta cattgggcgc
      421 cgtgaccact tcgatataac ctttgatgga ttcgatcgcg cgtttgacga catccattcc
      481 ataaccgcgc ccggatatgt cggtgatcag ttccgatgta ctgaacgccg gaagaaaaat
      541 cagattgatc agatcgtttt ccgaaaaacg cgagatatcc tgttcgtcgg ttacgatttt
      601 cttttcgatg gctttggctt tgatgcgttc gagatcaatg ccccgcccgt catcttcgac
      661 ttcgatcaga atggtattac cttcattgta tgcggcgata cgcagaagac cttcggccgt
      721 tttgcctgcg gcgatgcgat ccggcgacgg ctctagtccg tgatcgatcg cgttgcgtat
      781 caggtgtatc aaaggctcgt tgaggctttc tacgatttta cgatcaagtt cggtttccgc
      841 accttccacg acaagcttca catctttgcc taagtttttg gcaagatcgc gtacggcacg
      901 cggaaatccg tcgaatatgc tgcggatcgg caccatgcgc attcccatga taccggtttg
      961 tatggcattc aaagacaaat caaaattggc cgcttcttcg cgtgcgattt tgatcacatc
     1021 ggccaactca tccgccgtct ccgataaacg ttgggacagg tgcggattat caatgacatt
     1081 gccggcttgg gcagcgtttt cgtaatcgcg gagttctgaa ttccaatatt tgagtgccag
     1141 ataaattttg cgcagtacgg caatatgctc ttcggttttg atctgtttgg tcgaaatatc
     1201 accgacggac ttgatcaatt cgtcgagctt atcaatacgc acgtgaacaa actgatctga
     1261 attgagcact tcttcttttt gcgtaatacg gccggcgttg atttgcgttt gacccaacag
     1321 ttcacgcgaa gatttttgtt ccatcaaaag ctgattgagc tgatcgcgat tgatataacc
     1381 caaagcgacc aatctttcgc ccagaggcat gcgggtatcg gtggtctgat tggcgtgcat
     1441 cagttcttcg cgcgtaatca atcccgcttc gatcaggcgt tcacccaaac gatccggtcc
     1501 cgacgccccg cttttgactt cgcgtacggg ccgcgtttca cccacgggat gcgccggttt
     1561 gcgaatcaaa tcccggacag cttcttcggt aggcacggca cccgcattga tttcatccag
     1621 catgtccatc aaggcggcat tttgaaattc atccttttcg gtaatgcgga ttttttcgat
     1681 cagatcatct aaactttgat acgccattac caacacctgg tcggcgccag cactccatgt
     1741 caattttttt tctttcaatc cgcgaaagaa gaattcggca cgctgactga tattgcccac
     1801 gttttcaaac ttcatcatgc gggcggaacc tttgagcgtg tgagcggcgc ggatagctat
     1861 ctccagcgac gcggcatcgt gctgtttttg ccaggcggcg gtatgttgtt tcaaattacg
     1921 aacatgttct tcggcttcgg tgagaaaccg tttgataaaa agcccgcgat ccagcgttac
     1981 cgttttgctc gattttttgg gcgtgaccgg ctcggcgacc ggttccgtcg tctcggtgat
     2041 ttttttatcc gatgtgtcgg cgcttgcttc tttttgcagt tcgttgattt ttcgttcggc
     2101 gatgagcgtg atgcgatgaa gccacgacga aagatcggct tcggaatgtc cgtcgcgaac
     2161 gatatcgtcc agatgcgtat ccgtcttatc aagcgtgcgc aaaaacgtat ccatcacggc
     2221 acgttcatgc atgtactttt gtttgtgcgc attaccgaac gcctcctcaa acgcatgcat
     2281 cagctcgctg atcgcgcgca gattgagcat acgcgccgaa cctttgatgg tatggagcgt
     2341 gcgcaggatt tcatccggtg catcggtatc gcccggattt ttttcgatac ggagcaacgc
     2401 attgttcaaa cgccggatat tttcccgtgt ttcgagtaca aacttttgaa taaatgtgct
     2461 gcgatcgaag cgcgtgttca tttcaaatat tgtttacaag cattgatcag ataatcggac
     2521 gaatacccct gcgccaaaaa agacaccgaa gtatcctgtt tatgttctaa aattttgatg
     2581 gtgttgcgaa attcacgccg cgcatcgtcc gttttcccgc tttggcgata taccgaagcc
     2641 aaataataat gcgccatcgc gaggtctttt ttcaaaaaca gcgctttttt caatttttcc
     2701 gttgcggcgg caaattgtcc ggcatggtaa tctaccagcc cgcttaaaaa ataaacctcc
     2761 gcattgagcg catcaagcgc catcatcgca tcgcaggttt tgcgcgccaa atcgaaatct
     2821 tcctgcccaa tatagatgaa agcttttaaa atcaaaaaag gaattttagg ttctagcccg
     2881 tcagcgtgca tgtcccataa tgccagaatc tgtgcatacg cgccgtcctt gtacattgca
     2941 aagacctgat ccaaactggg cggcgcttcc ggtttggtgc gtgacgacgc ggatcggggc
     3001 gtttgcggtt tggcgctttc tttcggagaa accgggcgat ggtgacgttt cgattcgtga
     3061 agcgaaggcg ctatcgtttt tttggatttt tcttcggttc ccgttttttt ctgatacaaa
     3121 aagacaccgt cgtaatccac cagatccaaa tggccgaaat tatgatgcaa cgtctcggat
     3181 gaacccaaaa acagatatcc cgattcattg agcacttcgc tcaagcggcg attcaccgat
     3241 tggattttat ctttgggaaa ataaatggat acgttgcgga aaaagatcac atcaatgtcg
     3301 tgcagatagt ccggataatg atcgtcaaac aaattgagat attcaaaacg cgttatggcg
     3361 cgaatgcgct cgtctatgat ccggtaatcc ataagccgtt gatcagggat gcgaaaatag
     3421 gtgttaagat acgaagggtc tatcgcacgg aaagaattgc ggccaaaatt accgagctgc
     3481 gctatcgtca acgcgttgcg atcaatatcg gttgccagca cttcgatctc aaaatcaccg
     3541 agcaacatgc gcgtttcgaa aaggctgatg gcgatggtat aagcttcttc gccggtcgag
     3601 cagcccgcgc tccacacgcg aatccggcgc tctttctttt tacgaagcaa ctccggcacg
     3661 atgtgagact tgagtacgcg aaaatgggcc ggctcgcgaa aaaaatacgt ttcattattg
     3721 gtgatggatt caatgaattt acgcaattca tcttcgccgg cttcccggcc tgcgatgtgt
     3781 acgtggtagg aataaaaatc actaaatccg gtctgcgtca tgcgctcacg gattttacgt
     3841 tccaattcgc tgcggctgtc atcgggtatg gcgattccgc agaaatcgag gatcgttttc
     3901 ctaaacgttt caaataatgc gcgggtaaat tcctgttgca acggttatcc tcaggcgtcg
     3961 gttttgatac ccgtccaacg ggttatttgt tcggcgatac gctccgccgg taaaacgatt
     4021 tccgcggcgc ctatgtctat cgcggctttg ggcataccga aaatcagcga agtcgcttcg
     4081 tcttgtacga tcgtatgtcc gccgttttgt tttatagcca gcataccgtc ggcgccgtcc
     4141 cggcccatac cggtcaaaag tacgcccacc gcatgccgct tggcatgggt cgccagcgaa
     4201 cgcaaaagaa ccgaacccga cggtttgtgc ccggatacgt cttctttttc actcagaacg
     4261 attttgtttt gcgctccgat ttccaggtgt ttaccgttgg gagcgatata taccgtaccg
     4321 cgtgccaacg cctcagcctg ttcggcggac ttgacccgaa gcggcgacac gtcggcaagc
     4381 cactggatca gaccgtcggt aaacccgtcg gctatatgct gcacgaccaa taccggcgcg
     4441 gaaaggtttt tgggcaaaga acccagtatc gaagccagcg cacgcgggcc gccggtggac
     4501 gcaacgatgc cgatgacaag caaaaaatca ctcgtatcgc tgacggtttt gacatccacc
     4561 atcttgcctg agatatgcgt aatcactttg acccgtgcga tcaattttac tttgcgaata
     4621 aattcttcgt atttacgcgc atgaaaatcc aattcgaggg agggcttctc gaccacttcc
     4681 agcgcgccaa acgataacgc ctgaaacgca atgctcgcat ccgtactgga ggtgatcacc
     4741 aaaatcggcg tcggatagtg ggccatgatt tgtttgatcg catcaaggcc gttcatgatc
     4801 ggcatctgga tatccaccgt gatcacatcc gggcgcagcg tttcggccat agccacggct
     4861 tcgcggccat tgaccgcttt accgacaata cggatatcgt catcggtttc caaaatcgtg
     4921 gcgataatgt ccacggcggt cggcgaatca tcggcaatca gaacacgaat ctgactcata
     4981 cgaataaaaa aggtaggtga gaacgcgttt tgaatatggt gaaaatatat atcagacggc
     5041 ctgcatatac aaggtttagt ctacaaatat cgcgctgttt tccgcatccg gttaaaattc
     5101 ttgcattacg ccggtatcgt gcctattttg ggcgcatgaa atctttgccc atgttacgca
     5161 ttcccatcgt tcgctactat agtctgcact tgcatacgca tgcaccgcac gatacggtac
     5221 cataccccga cttgtacgcg tcatttgcgc agaccgcgta ttctcaaatg atcctcagcg
     5281 atgtcgcttt tgatctaaaa acgatgcaaa agcaaacgcg caatacggcg ttggacgaat
     5341 ttcaggtgat cgaaaattat acggccaaat tagatgacca ttcgacgatc aatatcacca
     5401 tctacgaagc ctacgatctg cccaaagatc ttttgatcgc cgatttatcc gaagagcttt
     5461 tggaaacact ggaaatcatt tatctttttt ataaaggcgc tattttcccg ggcattcaca
     5521 aattagaaat gttgcttgat atgggcgtca tcgccatgtt gccgtttcgt gaaattttgt
     5581 cgcgcgtgct cccggatatg ctcaaaatca atatactcgc cgaataccag ctgatgaatt
     5641 ttgacaaaca cgacgacgat tttattgatt tcaatctgat ttggtttctc ggaaaactca
     5701 atctgcagac cgtggacaag tttctcagcg ataacgattg gcatcacgac acgcctgatt
     5761 ttaataaccg cgacgaagaa tccccgccgt tcggcgatgc gtacgatccc gccgagtcgg
     5821 aatccaatga cgccgacgcg tcggccggtg catttgatct caatgaagaa gatcgtttgc
     5881 taaaaaccga attggaaaac gaactcaaac atttttatca ggaccaatcg catttttata
     5941 cccgcgtcgt cggactgcgc catacggagt ttgacatcag cacgctgagc gaaggtgcgc
     6001 ggctgacgct cgtgccggaa cccgaaaata cgcacgaccc tatggccgtg agcgtgcaca
     6061 gcgcccgcgg tgaaaaaata ggttacctca aaaaagaact ggcggcctta cttttttcgg
     6121 aattgcgcca aggcgtagtg tttcacgcca ccgtcgtcaa agttttgccc gaagccgaag
     6181 aagccaatct ccgcttgcat atactggtcc ggaagtggac gcatcatttc aactaaccgg
     6241 actacgctct cccgcgcaca caccatggac atttcaacgc tcaatcacgc tgtactcgcg
     6301 gccaaaccgc atctccaaaa cggccgtgtc gccgactata tcccggcttt acgcgaagcg
     6361 gatgcgcaac atttgggcgt agccgtagcg gacaccggcg gtgcagtgat cggtgccggc
     6421 gactgccagg tttccttttc gatacaaagt gtttccaaaa ttttttcatt agcctgtgtg
     6481 ctccggcagc gcggtttgga tgctgtactg cagcatgtgg gtatggaacc cagcggcaat
     6541 ccgttttatt cgctggtgca gcttgagtac gaatccggca aaccgcgtaa tccgtttatc
     6601 aatgccggcg ccatcgccgt gaccagttta ttacccggtc agacggccgg cgaaaaatcc
     6661 gaatttttat gccgcttcct gtccgagatt tccgggactc cgatcgcggt caacgaaaaa
     6721 gtttatttct ccgaatacga aacaagccat cgtaaccgcg ccgtggcgca ttttatgaaa
     6781 cattttggca atcttgaggg caatgtcgaa gaggctgtta acgcttattt tcagcagtgc
     6841 tcgatcacga tggatgcggt tacactggcg cgcctcgggc tatttttggc gcatcacggc
     6901 acagatccgg tttccggcaa gaccatcgtg gatgccaaaa gccagcgttt cttattggcc
     6961 atgatgacca tgtgcggctt gtatgacgcc tcgggagagt ttgccgtacg tgtgggttta
     7021 ccggccaaaa gcggcgtggg cgggggtatt tttaccgtcg tgcccggccg ttatacgatc
     7081 gtcacattcg ggccggcttt agatgataaa ggcaacagca tcggcggcat tcaaatatta
     7141 gaacacctct cttcggcctg ccaactttcc tcctgggtat aagcatttcc cttgttatta
     7201 acgacttcat acttgaattt taattggctt ttagcaaaaa gcgtttggtt tgcagactat
     7261 gttgtgaccg ttatgtgacg cataacataa tatcgaaata tcccttacca tttctctttg
     7321 cgacttggcg aaaaaaaata ttctcgctaa gacgccaacc cctaccctgc cgttcccgcg
     7381 aaaggggaat gaccgttgtg tatgagcacc cgcgtgcgcg ggtgagacaa tgtaaaatca
     7441 cacctgtttc tattttctct ttgcggcttt gcgagaaatt atacaagctc gccaaggcgc
     7501 taaggcgcga aaaaaacatc aagaccatat aattgagttt catgcgtttt aacgatgcgc
     7561 tttggggtcg ctggttacgt tttgaccgtt atgcgatcat taataattta cgatatatcg
     7621 ccaaaaactt accaaaatca tttgaccgcg atgggtattt tgcctatctt cggcgcgcgt
     7681 acagcctatg tgattttgca tattttaaca tattatttcg gaggattttt taatgaatat
     7741 tatcgtctgc atgaaacaag ttccggacag cgaaacgcgc gtaaaaatcg gcgccgacgg
     7801 caagtctgtg gatctttcgg cggccaattt tgtggtcaac ccctatgacg aatttgccat
     7861 cgaagaaggc attcgtctca aagaaaaatt tggcggtgaa gtgacgatcc tgacgctcgg
     7921 tacggatcgc gcggaaaatg acattcgtaa aggtttggct atgggcgcgg acaaagcgat
     7981 cctgctcaaa gccgatgcgt ttgacggcga tgtagcccat gcactggcgg aagaaatcaa
     8041 agccggcgcg tacgacttgg tacttttcgg caaacaagcc attgacgaag acagctccgt
     8101 catgccgcaa atgatcgctg aaaaactgaa tctcccctgc gtcacggtgg taaccaaatt
     8161 agatgtccag gccgagacca aagcgatcac ctgcgaacgt gaaatcgaag gcggcaaaga
     8221 gatcgtcgaa ctttcgtatc cggctgtgat cggcacccaa aaaggaatca atgaaccgcg
     8281 cttgcctaac ctcaaaggca tcatggcggc caagaaaaaa accatagata aaaaagatcc
     8341 ggctaaagct ccggcgatga cggaaatcgt agcgatcgaa ttgccgccgg cacgtcctgc
     8401 gggcaaaatc gtcggcaaag gcgccgatgc ggttcccgag ttgcttcgct tactgcacga
     8461 agaggccaaa gtcatctaag cggttcacga tccgatcaac aaaaaaaata atatggagta
     8521 tatatgtccg ttatagcatt tgcagaacag cgcggcggcg cttttaaaaa atcggctttc
     8581 gaagccgtgg ccgaggccaa acgtctggcc gataaactcg gtacgcaggc cgtcgccgta
     8641 gtcatcggaa ataacgtctc cggcatagcc gccgaactcg gcaaatacgg cgcttccaaa
     8701 gtctatgtcg cagaaaacgc ggcattggaa aactattcga ccgaaggtta cacgcaagct
     8761 ctgaacgaag ccgtcaaaaa agaaaacgct gcggtggtac ttttgggtgc gacggcgatg
     8821 ggcaaagacc ttgcgccgcg tacggctgca cgtctgggcg ccggtatcgc cagcgatgtc
     8881 accggtttgg acgtcgaagg cggtaaaatc accattaccc gcccgatcat gtccggcaaa
     8941 gccttccaaa aactgcgcat tacgacagcc acccaagtga tcacgattcg ccccaatgta
     9001 tttaaagcga ccgagtccgg cgcgaccgcg gcagtagaaa atctgtcgat ggcgatagct
     9061 cccatccgtg ctacggtaaa aaacacggtc gtcgaatccg gtaaaaaagt cgaactcact
     9121 gaagccgatg tcgtcgtttc cggcggacgc ggcctcaaag cgcctgaaaa ctggaactta
     9181 gtcgtagaat tggccgaagc tttcggcggc gcccacggcg catcacgtgc ggtcgtagat
     9241 gccggatggc gttcgcacga agagcaggtc ggtcagaccg gtaaaacggt cagcccgcaa
     9301 ctctatatcg cgtgcggcat ttccggtgcg atccagcatt tggccggtat gtcgagttcc
     9361 aaatacatcg tggcgatcaa tagcgatgcg gaagcgccga tattcaaaat cgccgactac
     9421 ggtatcgtag gcgatgcgat ggagatattg cctgccatga ccgcccacgt gcgtaagatc
     9481 aaaggcaaat agtccgattt atttttagca tgccagggct gacgcataag cgatcagccc
     9541 ttttttgttt acgaagtata cctgccggcg gaaaaaatca ttgcaaaata agccttagcc
     9601 gcctatcttt gaccggcttt tattttatct tcgtacgaca ttcgggcaat tagctcagtt
     9661 ggttcagagc gttcgcttca cacgcgaaag gtcactggtt cgaatccagt attgcccacc
     9721 aattct
//
LOCUS       DAOQGF010000118         9449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3413_length_9449_cov_6.682563, whole genome shotgun sequence.
ACCESSION   DAOQGF010000118 DAOQGF010000000
VERSION     DAOQGF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3413_length_9449_cov_6.682563"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK937_13175"
     CDS             complement(<1..217)
                     /locus_tag="PK937_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNH33571.1"
                     /translation="MNFESIVESVSRSVQLNREEIEYFTSLLECKVVKKKNFLLMTGD
                     ICLYDYFVVSGCFKVCYSDDTGTEFIVK"
     gene            complement(274..1017)
                     /locus_tag="PK937_13180"
     CDS             complement(274..1017)
                     /locus_tag="PK937_13180"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNH33572.1"
                     /translation="MNYRVILVSVMTLIMTSVFILSVKPEAKAVEVSAESILIENFQT
                     STTGQFPKGWGWLDGTSVKKINEAKPGEVPYVVMEENGNKFLRADDKGQAITIVSDKK
                     WNIKKYQCIQWRWRVNQFPTGANEYAKGKTDNAASLYISYYVSFIGIPRSIKYIWSNT
                     LPECETFRKDGTGKATNVVVESGTSKTGQWITETINIYEQYKRVFGEYPPDEVAGIAI
                     RTDADGTNSRAIADYDDIIAIPYCDGPCK"
     gene            complement(1037..1681)
                     /locus_tag="PK937_13185"
     CDS             complement(1037..1681)
                     /locus_tag="PK937_13185"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNH33573.1"
                     /translation="MEDAVVKPYYVVDVKRKSTTFEVAAMAKIRNQFCERYPALAERF
                     LAVEMIHKDEIKPNSEVTEYFQLLASKTRIGSQYDWLARFAHQFQIDALELCIEKFDS
                     DGSRIFGDYIVPLLKGVGHECRVEGPFTEPGMRLFTCFRFPIIHIEKNEMRRIAEKQG
                     FVDLMRLIWFCHHPAKDGKPCGKCRPCQLANGSGMTYEFSKVSLLEKTINFFKS"
     gene            complement(1765..3117)
                     /locus_tag="PK937_13190"
     CDS             complement(1765..3117)
                     /locus_tag="PK937_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH33574.1"
                     /translation="MIGRTLVAMSKSGMKYVTIICRMSDRTLIERCVKEVDAERISVS
                     PTIYSMDDDVNLSKAVNEITKSSESTWMLMTYNKIIHPTFILPARECVQTPALITYNG
                     VMRQGDKVIFGEEMAPKFQTQFQDVSIFEMVSVQDNKVKTWNTADAPHFSAGILVVRN
                     TDIVLAETRQSISELIKFFIKKELLKTVSVSNGWCMPVNKSIASSVIKNVFWKMAFKE
                     ISGEFSKAVNSKLSKPLTFWFSAHQYKPDTISNMSLVLFIVASGFLLIPEYWAMVVFA
                     VVWQFSAGVLDRCDGEVARMRNHESEAGGKFDMIVDDLRFALPLMALGAACFLELPDV
                     RYVIVAAITLAGSFAITFFEQATMRRFGYVSRQKLNVDYLKAKGDTSRFSATMKKLQP
                     VFKGDIRTFYVFLLTFLGEKTIIFWVLVFYHVFVSAVALFGALTFPQLIKKIQSETKR
                     "
     gene            complement(3261..4271)
                     /locus_tag="PK937_13195"
     CDS             complement(3261..4271)
                     /locus_tag="PK937_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH33575.1"
                     /translation="MSQLRVREEERIIIIDSNIIADDRLIKYLGNHTDSLAITNQYHI
                     VGVNTSRLVKNNIDVFSFETAKKYVANFDPKVIEPNEMNKYVHELRSQLAPYFFVCTH
                     AMDIRQMQNIMYEANFKGTLDFIATYVYKYPVREITKLLSRHPWVTPDFITVLSIASS
                     FAVPLLLAFGEMGWAVLVGWLMFIFDSVDGKLARLTVRLNPTMGVIEHATSAPAIFLW
                     FAGLGWYFSNGQLLEFTHPASATAWSLMALYWLDKGVNGIFKPRFGIDLYNYAPIDRF
                     FHLFACRRAIIHLIITLGWATGYRDEAFYFLGIWMVISFMFHLFRCGWILATDEPQTV
                     AE"
     gene            complement(4493..5683)
                     /locus_tag="PK937_13200"
     CDS             complement(4493..5683)
                     /locus_tag="PK937_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH33576.1"
                     /translation="MKALIDARNAAGMLKLFDITLVERLLRQVWVRGIREIIILGSIN
                     PSVFRTEFSKRFPMTVTIIEQYEEWLSKTGDLVILQSDVLYDDRILDVLTQTQDDRLI
                     GDFNNPSCPYGAHLSGINITVEQVLLENNVRRLFSNENIQDIHDMDVYIKFLRKSYVP
                     TLKRIHVGDDLTDIENDLFEKTFKSGLEWIAIYGYKTPVRELTRLFSKTNITPNHVTA
                     VAVFCRWVSIPFLFYGWIGLGLGLIVLFVLLDSLDGKLARMTFRFSDQADWIDHGSVL
                     PTRLGWYAGLGWHYSSGVWSSQAGMLTIITLVCIVLDDLNWAVAKRIFKRTLFDITDF
                     DAKAHLFTHRRNDMFLMLSGYLIGLGLYSFYLICGWVVVTWIWHCTRIVYLSNDSSLS
                     KERN"
     gene            complement(5667..7109)
                     /locus_tag="PK937_13205"
     CDS             complement(5667..7109)
                     /locus_tag="PK937_13205"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNH33577.1"
                     /translation="MSKSFFLKQAAWFKDSIREFSVPDEFEVDIINPNDAPPIDNKAI
                     AYAIDHPFGASLNERLVGKCRVVFVVDDLGRPTPAHDIIPVIIPYLTKVGIKDKDISF
                     VIATGSHRQLSIDEMKLKLGSTVAEKYSVHCHDAFNSKLVHLGTLENGFPCIANKIVA
                     EADIIIGIGCVIPHACHGFGGGAKLFCPGIAGMESIVTMHGFTPKRGRVVMDIPEGEW
                     DMRSASEAFAEKLPPIFLINVVMNSQREMCGIFAGNINDTFRAARTMAAQVYRTEIDP
                     KKAQQYDVVFVNMYPLDSDPVQSGKSPWIKKFFKNAVAIQINSSADGIDYHGWKLLRR
                     SRITTLIILLIGEGLSLKFMPSIFRKPASWSVLKKLRSYCVRKMLDNPGVDYASFIEK
                     WSSKSKIENKDFVEKKELIPSLSSSPWIFSENYPPLQFQKKYPSGTLLQSWDRVMDVI
                     RHYYPKAKIAVVTCAPVQIPVLKGNDESVD"
     gene            complement(7111..7758)
                     /locus_tag="PK937_13210"
     CDS             complement(7111..7758)
                     /locus_tag="PK937_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNH33578.1"
                     /translation="MSRRIAFFDIDGTITRHSSERLFIRYLLQKKIISYSDLVLATLR
                     YSCKHPTYLKNGFKQNKMYLCGLDAEMIRQHASQCFDEYIRPSIKPIMTEALVMCRKD
                     GYTIVLLSGSLRCLAEPMQRFTQAEDLICSETEIINNRYTGEMSSLHPYGENKKTLAI
                     RYCTDHQIAVRNCRAYANEWADRFLMEAVGEAVAVDSDEKLKRLAIEKKWSVLKT"
     gene            complement(7755..8405)
                     /locus_tag="PK937_13215"
     CDS             complement(7755..8405)
                     /locus_tag="PK937_13215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH33579.1"
                     /translation="MIRLLNLANSITIARIGVLYITVVLIYTQRPMWLIIAVLLAILI
                     IVMDALDGYVARKRNEVTQFGGVLDITGDRIVENVFWIVFADLDIIPMWIPIIVMSRG
                     FMTDAIRSQALADGKTAFGENTMMQSEIGKFLVSGRFMRAFYGVIKAITFPYLILVLL
                     AKEQHLRDANLQDYLWLSSLTYNGGLVLAIITTAVSLLRGIPVLLEGKQFFVKDKA"
     gene            complement(8402..>9449)
                     /locus_tag="PK937_13220"
     CDS             complement(8402..>9449)
                     /locus_tag="PK937_13220"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNH33580.1"
                     /translation="IMPATVTRAMFGFFLSLLYGMTAWIFEAELPAELRALNMAWVIV
                     FACIGIFFEGLGTITEAIFAVKQRFGMNSFLGSAQTLVEQIFALLFFVQFGLTYYFVG
                     VSIAQAVMFLLRIWYIRDVVFQFRPNFYRWNSAKKMLKEYFPFYVRRFFRLGFLQGEQ
                     IAVAFLLPLEQLANFNLAKRTAKFMKPYIEAFLNPLIIKLSKARDITLMKEYRKTFLW
                     FTMPVPVILALASPWIIQIYGEKYAASWPIMAIFSLSWIFYALSTLQLGVVGIFGEPK
                     EALMADAFAGIFGLSTTILLILLFKEYGLAWGQVISFATLYISGLYIARKYYRNPQLI
                     KPVTVQPITQENPS"
BASE COUNT         2899 a         2176 c         1722 g         2652 t
ORIGIN      
        1 actttacgat aaattcagtt cctgtatcgt cactataaca taccttaaaa caaccactga
       61 caacaaaata atcatacaga caaatatcac cggtcatgag cagaaaattc ttttttttta
      121 caactttgca ttcaagcaac gaagtaaaat actcaatttc ttcccgattt aactgtacgg
      181 atcgcgatac actttcgact atagactcaa agttcatgag aaaatgctgt ttttttataa
      241 aaactgatgc gagtaataaa tcatgttata aatttattta caaggtccgt cgcaatacgg
      301 aatggcaata atatcatcgt aatcggctat cgcacgcgaa ttggtaccat cggcatccgt
      361 acgtatcgct ataccggcga cttcatccgg cggatattca ccgaatacgc gtttatattg
      421 ttcgtagata ttgattgttt ctgtaatcca ttgaccggtt ttggatgtgc cgctttccac
      481 gactacattt gtcgcctttc ctgtaccatc ttttcggaaa gtttcgcatt ccggaagtgt
      541 attactccaa atgtacttga tactgcgcgg tatgccgata aaactcacat aataactgat
      601 atacagactg gccgcgttat cggttttacc tttggcatat tcattggcgc cggtaggaaa
      661 ttgattgacg cgccagcgcc attggatgca ttgatatttt ttgatattcc attttttatc
      721 ggacacgatc gtgatggctt gtcctttatc atcggcacgc aaaaatttat taccgttttc
      781 ttccatcacc acgtacggca cttcaccggg tttagcttca tttatctttt taaccgacgt
      841 tccgtccaac catccccaac ctttcggaaa ttgaccggta gtagaagttt gaaagttctc
      901 aatcagaata gattcggcag aaacttctac ggctttggct tccggtttga cgctcaatat
      961 aaaaaccgat gtcattatca aagtcataac agaaactaat ataacacgat agttcataac
     1021 atccctcttt cgtatattac gacttaaaaa aattaatcgt tttttccaat aaagatacct
     1081 tcgaaaactc gtacgtcata ccggaaccgt tggcaagctg gcaagggcgg cacttcccgc
     1141 acggtttacc gtcttttgct gggtgatggc aaaaccaaat cagccgcatt aagtcaacaa
     1201 atccctgttt ttcggctatt cgccgcattt catttttctc tatgtgaata atgggaaacc
     1261 taaaacaagt aaacagcctc ataccgggct ccgtaaacgg tccttcaact cggcattcat
     1321 ggccaacgcc tttcagcagt ggaacgatat agtcgccaaa tattcgtgat ccatcagaat
     1381 cgaatttttc aatacaaagc tcaagtgcgt caatctgaaa ttgatgcgca aaacgagcga
     1441 gccaatcata ctgagaaccg attctcgttt tggatgcgag aagttggaag tattcggtca
     1501 cttcgctatt aggtttgatc tcgtctttat gaatcatttc tacagcaaga aatctttcgg
     1561 ccaatgctgg atagcgttca caaaattgat ttcgaatttt cgccatcgcc gccacttcaa
     1621 aggtggtgga tttccgttta acatcaacta cataataggg tttcacaacc gcatcttcca
     1681 tcagtagcgc ttccaaaaca cgaaaactgg aatcccatcc gccggtccaa agcacatgta
     1741 tagttttcat attttccatc cagtttagcg tttggtctct gattggattt ttttaatcaa
     1801 ctgcggaaac gtcaacgcac cgaataaagc aactgcgctt acaaatacat gatagaacac
     1861 taatacccaa aaaataatcg ttttctcgcc gagaaaagtc aacaaaaaca cataaaaagt
     1921 acgaatatcg cctttgaata cgggttggag ttttttcatt gtggcgctga agcgactggt
     1981 atcgcctttg gctttcagat aatctacgtt cagtttttgg cgactcacat aaccaaaacg
     2041 gcgcattgta gcttgttcaa agaatgtaat agcaaaactg cccgcgagcg tgatcgcggc
     2101 tactatgacg tatcgcacat ccggcaattc caagaaacac gccgcaccta acgccatcaa
     2161 aggtagagca aatcgtaagt catccacgat catatcaaat tttccgccgg cctcggattc
     2221 atgattacgc atacgtgcta cttcgccatc gcaacgatcc agcacgccgg ccgaaaactg
     2281 ccaaactacg gcaaaaacga ccatagccca gtattccggt attaataaaa acccggacgc
     2341 tacaataaaa agcacgagcg acatattact gatcgtatcc ggtttgtact ggtgtgcgct
     2401 aaaccaaaat gtaagcggtt tggatagttt actattcaca gctttagaaa attctccgct
     2461 gatttcttta aaagccattt tccaaaaaac gttttttatt accgacgacg caatagattt
     2521 attgaccggc atacaccaac cgttgctgac agaaaccgtt ttcaacaatt ctttctttat
     2581 aaaaaacttt atgagttcag atatactttg ccgagtttct gctaaaacaa tatccgtgtt
     2641 acgaacgact aaaattccgg ctgagaagtg cggcgcgtcg gccgtattcc atgttttcac
     2701 tttattatct tgtactgata ccatttcaaa aatcgaaaca tcctgaaatt gggtttgaaa
     2761 cttgggcgcc atctcttcgc caaaaataac cttatcccct tgacgcatga cgccattata
     2821 cgtgatcaat gccggcgttt gtacgcattc gcgtgccggt aaaataaaag tcggatggat
     2881 gattttatta tatgtcatca gcatccatgt gctttctgat gattttgtaa tctcattaac
     2941 ggctttactt aaatttacgt catcatccat actatagatc gtaggagata cactgatacg
     3001 ttcggcatcc acttctttaa cacaacgttc aatgagtgta cgatccgaca ttcggcatat
     3061 tatcgttaca tatttcatgc cggatttact catcgccacc agagttcgtc cgatcaacgt
     3121 ctctccaccg tacaacgtat caaaatccgg cgcgtcgttt gttggtttta ttgcaattat
     3181 tatcgcgttt tgaatcatca ctcaacaggt ttaaactatc ttattaattt cttttttgta
     3241 gcaataatca cacagtgtat ttattcggcc acagtttgag gttcgtccgt agctaaaatc
     3301 catccgcaac ggaacaaatg aaacataaaa ctgatgacca tccaaatacc taaaaaataa
     3361 aacgcttcat cgcgatagcc ggtcgcccac cctagggtaa tgataagatg tataatcgcg
     3421 cgacgacagg cgaaaaggtg aaagaaacga tctatcggag catagttata caaatcaatt
     3481 ccgaagcgtg gtttgaatat tccattgacc cctttatcta accaatacag cgccatcagc
     3541 gaccacgccg tagccgaggc tggatgggta aactctaaaa gttgcccatt tgaaaaatac
     3601 cagcccagac cggcaaacca caaaaaaata gccggggcac tcgtagcgtg ttcgatcacg
     3661 cccatcgtcg ggttaagccg aacggtcagt cgcgccaatt ttccgtccac ggaatcaaaa
     3721 atgaacataa gccaacccac caaaacggcc catcccattt ctccaaaggc taatagtaac
     3781 gggacagcaa acgaactagc gatactcagc acggtgataa aatccggagt cacccaggga
     3841 tgacggctta acaacttagt gatttcgcgg accggatatt tatacacata ggtggcgata
     3901 aaatcgagtg tgcccttaaa gttggcttca tacatgatgt tttgcatttg acgaatatcc
     3961 atagcatgtg tacaaacgaa aaagtaaggc gctaattgcg aacgtaactc atgaacgtac
     4021 ttattcattt cattcggctc tatcactttt ggatcaaaat tagccacata cttcttggcg
     4081 gtttcaaagg aaaaaacatc gatgttattt ttaaccaatc tggaagtatt tactccaact
     4141 atgtggtact gatttgtaat tgccaacgaa tccgtgtgat tgcccagata tttgatcaaa
     4201 cgatcatccg cgatgatatt agaatcaata ataataattc gctcttcttc acgaactcgc
     4261 agttgagaca tatattgatt ccattcattg aacgggtgac agtatacatc cgagtgaaca
     4321 aacataattt tttcactcca tacattaatg gtatgcgctt ttaattcttt acactgccga
     4381 ataagccttt caacgaatgt tactccataa atctctcggt gagcatattg ctcaaaatca
     4441 ttcgtaagta atatatgggc tgttaatttc aacaatttta aatctcgatt tattaatttc
     4501 tttcttttga caagctacta tcgttcgata aatagacaat tcgcgtgcaa tgccagatcc
     4561 acgtgaccac gacccacccg caaattaaat aaaacgaata cagccctaac cctataagat
     4621 aaccgctaag catcaaaaac atatcattgc ggcgatgagt aaacaaatgc gcttttgcat
     4681 caaaatcagt aatatcaaaa agagtacgtt taaaaattcg tttggcaaca gcccaattga
     4741 ggtcatccaa aactatacaa actaatgtta tgatagttag catacccgct tgtgacgacc
     4801 atacaccaga actgtagtgc catcccaacc ccgcatacca gccaagacgt gtcggtaaaa
     4861 cggaaccatg atcaatccaa tcggcttgat cgctaaatct aaacgtcata cgtgccagtt
     4921 tgccatctaa agaatccaat aggacaaaca acactatcag acccagtcct aacccaatcc
     4981 aaccataaaa taaaaaagga attgataccc atcggcagaa aaccgcaaca gccgttacat
     5041 gatttggcgt aatgtttgtc ttggaaaaaa ggcgtgtgag ttcgcgtacc ggcgttttgt
     5101 aaccgtatat agcgatccat tctaaacccg atttaaatgt cttctcaaac aggtcgtttt
     5161 caatatctgt cagatcatca ccgacatgaa tacgttttag cgtcggcaca taggattttc
     5221 ttaagaattt gatatagaca tccatgtcgt gaatatcttg gatgttttca ttagaaaata
     5281 accgacgaac gttattttct aataatactt gttcaacagt gatatttatt cctgaaagat
     5341 gagcaccata agggcaagac ggattattaa aatcacctat caatctatcg tcttgtgttt
     5401 gagttaacac atcaagtatt cggtcatcgt acagcacatc gctttgcagt attacgaggt
     5461 cccctgtttt cgataaccat tcttcatatt gttcgataat ggtcaccgtc atcggaaatc
     5521 gtttagaaaa ttccgttcta aaaacggaag gattgattga gcctaaaata atgatctcgc
     5581 gtattccacg tacccacact tgacgaagca atcgttcgac aagagtaata tcaaatagtt
     5641 taagcatgcc ggctgcgttt cgggcatcaa tcaacgcttt catcgtttcc tttcaatacg
     5701 ggtatttgaa ctggagcaca agtcactacg gctatcttcg ctttaggata atagtgtcgg
     5761 attacatcca tgactctatc ccatgattgt aaaagtgttc cgctcggata ttttttttgg
     5821 aattgtagcg gcgggtagtt ttctgaaaat atccacggac tactactgag cgaaggaatt
     5881 agctcttttt tttccacaaa atccttgttt tctatttttg atttggaaga ccatttttcg
     5941 atgaacgaag cataatctac gccgggatta tcaagcattt ttctaacgca atacgatcgc
     6001 agttttttta atacactcca agatgccggc ttacgaaaaa tggatggcat aaattttaaa
     6061 ctgagtcctt caccgatcaa taatatgatt aaggtggtaa tccgactacg gcgaagcaat
     6121 ttccaaccat gataatcaat tccgtctgct gaactgttta tttgaatagc cacagcattt
     6181 ttaaaaaact tttttatcca tgggcttttt ccggattgta ccggatctga atcgagcgga
     6241 tacatattta caaatacaac atcatattgt tgagcttttt tcggatctat ttcagttcga
     6301 tatacctgtg ccgccatggt tctggccgcg cgaaaagtat catttatatt tccagcaaaa
     6361 ataccacaca tttccctttg agaattcatg acgacattga tcaaaaaaat gggaggtagt
     6421 ttttcagcaa acgcttcgga agctgaacgc atatcccatt caccctccgg tatatccatc
     6481 actactctgc cgcgtttggg ggtaaacccg tgcatggtca ctatactttc catacctgca
     6541 atacccgggc aaaagagttt agctccaccc ccaaaaccgt gacaggcatg cggtatcacg
     6601 cacccaatcc cgataataat atctgcttcg gctactattt tattagcgat acaaggaaag
     6661 ccgttctcca atgtccctag gtgaaccagt ttagaattga aagcatcatg acaatgaact
     6721 gaatattttt cggcaaccgt actccccaat tttaatttca tttcatcaat tgatagttgc
     6781 cgatgggagc ccgttgcgat aacaaaacta atatctttat cctttatgcc gacttttgta
     6841 agatatggga ttatcacagg aatgatgtca tgcgccgggg taggccttcc caaatcatct
     6901 actacaaaaa ctacccgaca ctttcccaca agccgctcat tcagtgacgc accgaaagga
     6961 tggtcaatgg catacgcaat ggccttgtta tcaataggtg gcgcgtcatt cggattgata
     7021 atatcaacct caaattcatc aggtacagaa aattctctga tagaatcttt gaaccaagcc
     7081 gcctgtttca aaaaaaagga tttgctcata ttacgttttt aatacactcc attttttttc
     7141 aatagctagt cgtttcagtt tttcatccga atccacggcc actgcttctc cgacagcctc
     7201 cataagaaaa cgatccgccc actcattggc ataggcccgg cagttcctca cagcgatttg
     7261 atgatctgta caataacgta tcgctaatgt ctttttgttt tcgccgtacg gatgcagtga
     7321 actcatttcg ccggtatatc ggttgtttat gatttctgtt tccgaacaaa tcaaatcttc
     7381 ggcttgcgta aaacgttgca tcggctcggc taaacaccgc aatgagccac tgagcaaaac
     7441 aatcgtatac ccatccttgc ggcacattac aagcgcctcc gtcattattg gcttgattga
     7501 cggacggatg tattcatcaa aacattgtga agcatgctgc cgaatcattt cagcatctaa
     7561 tccgcataaa tacattttat tttgtttaaa tccatttttt agatacgtcg gatgtttgca
     7621 cgaataccgc agcgtagcca ataccagatc ggaatacgat ataatttttt tttgaagcaa
     7681 atagcgaatg aaaagccgct cggatgagtg acgcgtgatc gtaccgtcaa tatcaaaaaa
     7741 agctatacga cggctcacgc tttatccttt acaaaaaact gcttcccctc aagtaaaact
     7801 gggatacccc gcagaagact cacggccgta gtgataatag ccaacaccaa cccaccattg
     7861 tacgtcaacg atgataacca caaataatcc tgtagattag catcgcgcaa atgctgttcc
     7921 ttggctaata aaacaagaat cagatatggg aaagtgatcg ctttaattac tccgtaaaaa
     7981 gcacgcatga agcgcccgga tactaaaaac tttcctatct cagattgcat catggtattc
     8041 tcgccaaatg ccgttttccc atcggctagt gcttggctgc gaatcgcatc cgtcataaaa
     8101 ccgcgcgaca tcacaatgat aggtatccac attggtatga tgtccagatc ggcgaaaaca
     8161 atccaaaaaa cattttcgac aatacggtca ccggtaatat caagaactcc gccaaactga
     8221 gtcacttcgt tgcgcttacg cgcaacataa ccgtctagtg cgtccataac aatgatcagg
     8281 atagctaaca ggacagcaat gataagccac atcgggcgct gcgtatagat caatacaacc
     8341 gttatgtata aaacgccgat gcgcgcaatg gtaatggaat tagcgaggtt caacaatcgt
     8401 atcatgatgg gttttcctgc gtgatgggtt gcacggttac aggcttgatg agttgcgggt
     8461 tgcgataata cttccgagcg atatacaatc cggaaatata caatgtggca aatgaaatca
     8521 cttgccccca cgcaagtcca tattctttaa acagcaaaat caaaagaata gtagtgctca
     8581 agccgaagat gcccgcgaaa gcatccgcca tcaacgcctc tttcggctct ccaaaaattc
     8641 caacaactcc aagttgcaag gtcgaaagtg cataaaaaat ccacgataaa ctaaaaattg
     8701 ccattattgg ccaactggcg gcatattttt caccgtatat ctgtataatc cacgggctcg
     8761 ctaaggctag gataacaggt accggcattg taaaccacaa aaaagtcttt cggtattctt
     8821 tcattaatgt aatatcgcgc gctttggaca atttgatgat taaaggattt aaaaaagctt
     8881 caatatacgg tttcataaat ttagccgtgc gtttggccag attaaaatta gctaattgtt
     8941 ctagcggcaa aagaaacgcc acagcgatct gttcgccctg taaaaaacct aacctgaaaa
     9001 accgacgcac ataaaatgga aagtattctt taagcatttt tttagcagaa ttccaacgat
     9061 agaaatttgg tcgaaattga aatacaacat ccctgatata ccaaattcgc aataaaaaca
     9121 tcactgcttg cgcaatactt acacctacaa aataatacgt caaaccaaat tgtacaaaaa
     9181 acaaaagtgc aaatatttgt tcaaccaaag tctgtgcgct acccaaaaag gaattcattc
     9241 cgaaacgctg tttgaccgcg aaaatagctt ccgttatagt tcccaatcct tcaaaaaaaa
     9301 tacctatgca agcaaatacg atgacccaag ccatgttgag cgcacgaagt tcagcaggaa
     9361 gttcagcttc aaatatccac gccgtcatac catacaacag tgaaagaaaa aaaccaaaca
     9421 tcgcccgggt tactgttgca ggcataata
//
LOCUS       DAOQGF010000119         9383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3446_length_9383_cov_6.574292, whole genome shotgun sequence.
ACCESSION   DAOQGF010000119 DAOQGF010000000
VERSION     DAOQGF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_9383_cov_6.574292"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PK937_13225"
     CDS             <1..1347
                     /locus_tag="PK937_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33581.1"
                     /translation="RVQDVAPIEYLNVRDASTLDAIGFGAGSVWLRSEDRYWSSSLSY
                     PSFSRSELPKTKTIEWNSIPTSSEIASRSIFVSNESGYIFIPHSNLFQDRELRQFPIT
                     GYLQDVNYNTWVTTAGAGAWFSNNGSSILSPLNYGLNASSVKAMAFDGADMWIGATAN
                     TLPGQYPSELSGLTQWNQKNDHFTYYFSSLHDNLALNEITDIEADENYIWVGTTSGLN
                     YLNKKSNQWTIAKTYSGLKDDHIIEIVPFQKGIIVATENGLHRSILTKKGIQFQPLEI
                     PDLWNRHVLKILPSGNSIWLGTDNGIYRLDSSFTIQNHYTALGYDIPLNSLVNQVVPA
                     ITEMANNLFFISTHSVLRFDKHKKEWESMPISGSYLQSGVYDAIADEDNLWIGLADGA
                     LQYDFELQRWAYYNKKDGIGGYSVYKILPDGDYVWFATENGLTQFYWNAEHLRSQK"
     gene            complement(1348..2523)
                     /locus_tag="PK937_13230"
     CDS             complement(1348..2523)
                     /locus_tag="PK937_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH33582.1"
                     /translation="MTPYIFIALSIWSLTISGCTSKENNKSHSDHADLYYCPMHKEVT
                     SDKPGVCPICQMDLVKKSRVESAADTMHHGLFLNPDQMRSAQVTTMKVRTSKLNKTIT
                     GFTTLEFAEDLRKTIAARFSGRIEKLYVAKTGDRIEAGQALFEIYSPELVQAQNDFVV
                     LLNNKEGQSYTNQMLSAAETKLRLLGVTSDQLETLRRTREIKQNFIYHSPYSGTVIEK
                     KITEGAYVNEGTILYDMADLSLIWGVSDIFESEARFLKLHSTVTIRLDTYPDEKFTGT
                     ITLIYPVIEPVSRTIKVRIEINNKDYRLRPKMYGQMIFDSPLPELPVIPETAVLLTGK
                     RALTWVMTSPHHFEQREIMLGEKVGDQYTVLSGITAGEVIVKTGGYLIDSESQLTGK"
     gene            complement(2540..5851)
                     /locus_tag="PK937_13235"
     CDS             complement(2540..5851)
                     /locus_tag="PK937_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNH33583.1"
                     /translation="MIENIIDWSARHRWMVLTAYVAISLYGIYSFREMSVDAIPDLSE
                     NQVIVYTEWMGRSPQVVEEQLTFPLVTALQGLAHVKAVRASSMFGMSFVFVIFDDNTD
                     IYFARSRVQERLSTVRGSLPKDAIPTLGPDGTGIGHIYWYTVEGGEQDLGTLRSIQDW
                     YIRYQLSSVEGVAEVASIGGHVKQYQIDLNPVRMRAFGIDIMDVTQALQRNNNEVGGK
                     IIEWSSAEYFVRGQGYIRTESDIRNLVLRNNGSGIPVLLGQIADVQIGGDIRRGSLEK
                     DGEGEAVGGIVVMRSGENAKAVIDRIKKKINEIAPGLPHGVVIKPSYDRSVLIEQSIE
                     TLNRALFEAALTVSLMVAVFLLHARSILRIIIEIPVSILLSFILMRIFDIHANIMSLG
                     GIILAIGVIVDASIVMVENAYRNIAQALETKGKLLTEDYQAIALQSAKQVGRAIFFSE
                     LIILVSFLPVFMLTGQEGKLFAPLAFTKTFVMLASASVVLTLIPVLMTFLMRGKFRPE
                     KENPVTHFFIKLYEPVMHWVLRYRKITLSLNVIALIITIPMIVNMGSEFMPPLDEGSI
                     LYMPVTLPNASLTEVNRILTIQDRLIKAVPEVAHVLGKAGRAETATDNAPISMIETII
                     LLKDKKDWRPGITKKDIIQELDSKLQIPGVRNGWTQPIVNRINMLATGVRTDVGFKIL
                     GNNADTLEYYAIEAEKILKNVHGASDVVAERGQNGYYLDITIKPEAAQRIGFTNGDLQ
                     EVIEMAIGGQNIGTVLDGRMRFPIRARFGREWRNDTEALKQLWIPIRPRNTMASATPI
                     ENLSYESTKTSMPASMTQITGVVSEPIRQSLLTDVNEGVIFLPLGQLAEVKVNQGPTM
                     VNSENGMLRSVVYMNTAGRDLDHVVEDAKVLIQSELKLPEGYTYQWSGQYEHKLRAQR
                     TLMIIIPIVFILIFLLLYFTVRDPIEALVVMLSVPFALIGGVYMNFILGYNFSVAVWV
                     GFIALYGIATETGVIMVVYLHEALDRRLQSKNGVITQKDIYEATIEGSVLRLRPKLMT
                     VGTALIGLVPIMWSTGTGADVMKPLTAPMIGGLITSAIHVLIVTPILFAIMKERALSK
                     GTLKKSKMAHWIKES"
     gene            complement(5844..7118)
                     /locus_tag="PK937_13240"
     CDS             complement(5844..7118)
                     /locus_tag="PK937_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH33584.1"
                     /translation="MSGSLKKIALIIGLASLEIPQVHTQVLIDSLIREAIQHHPKIHA
                     IQHNYEAYRFQAKQKKSWPTPDVGFELFQMPASNFPIPVKGNMETDYFIQQMIPWPGK
                     LEAMSEAAHYTAEIQKTMIASAQLNIERDARVAFFELCMINERRRNNANAQRTLEQLL
                     QLSLKRYEVGQITQADILQIENERLALENKAFELEKDRRVTESMLNGVLGNNEIRWPD
                     SIPVDWTNNFSVVLDTLIQNTLPKKPEIQGMNHEIAMFTAEKKAAHREWFPDIMVRGM
                     YKDMSMGKNYWSLMLSANIPFAPWSRGSLDARIKELDMHIKHGAMEIENMKIMITSEA
                     RSAIERIKSSRRQAEQIEQRILPHYEAAYQSMMISFQTGKADTAMLLSMLRMIYMTKD
                     DLVMALAGSKMAEADFYKAIGTSNMKSEAHHD"
     gene            complement(7191..7610)
                     /locus_tag="PK937_13245"
     CDS             complement(7191..7610)
                     /locus_tag="PK937_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33585.1"
                     /translation="MNNQSLQKVLSLLLALVWVTGCLANVRITGTCYSTKTNSMSCCQ
                     SSTTMAGTSNSLQIAAYKSCHCPGMMSVLPERGVSLPIETLKTNSVQQTHALLNFVFQ
                     TAFDQAYNKNSFFDFSHQHPPANTASETNALLSTFRI"
     gene            complement(7614..8762)
                     /locus_tag="PK937_13250"
     CDS             complement(7614..8762)
                     /locus_tag="PK937_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33586.1"
                     /translation="MTEKKRILVVDDEESIRLICEMALQQNGFKTFSASDGSEALKVV
                     ENQNVDLILSDIFMPMMNGFQLLQSLKKDPYYNQIPVIFLTAATDNDHIIEGLQLGAE
                     DYISKPIRIKEVIARVQMVLSKIEARHQTKPQPQQGHAVKADLDTRRENKPVGKLEEK
                     PLIDVIAFCEANSLTGELIVTHENRRGKLMYRKGELLEVSLDDKKDDAAIDELMSWQN
                     GTFEINQQLLTLDALTKSLRSDAKSTMSSKEGGMAPAPLGSKAVSIPADAVIAALNAA
                     TMHVGQSMGEAKVGNYFRRAQLAHVNKWNTLGYFVVDQRGKTTALKPIQLNGDDIVGV
                     SEWIKSFTRLCAEKDPQWSDMNVVQIVKNNIHDSHWKLLDSAGFPGYF"
     gene            complement(8759..>9383)
                     /locus_tag="PK937_13255"
     CDS             complement(8759..>9383)
                     /locus_tag="PK937_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33587.1"
                     /translation="SWFFKAQSHYSKQIYRSDAEVSLSAITPRNTRLIDIPAQLIWRH
                     IPDKITGASEFVVHLRSYENDYSFEETVLGNSFTFRNEMNMERGIKYFWFTEAVNENI
                     NEATESVWFYVMNKEERTEFENELRESNDAIELTEDTVLKKLLTATIYMRYQLYKDAL
                     EITASVLKEQPHHIMALKIQARIYEIIEQPVETKRLLEITQRYMENQ"
BASE COUNT         2583 a         2145 c         1871 g         2784 t
ORIGIN      
        1 cgtgtacaag atgtagcacc catcgaatat ttgaatgttc gcgatgcgtc aactcttgat
       61 gcgataggat tcggtgcggg ttccgtttgg ctgcgttcgg aagacagata ttggagttct
      121 tctctttcgt atccgtcatt ttcgcggtca gaacttccaa aaacaaaaac tatagaatgg
      181 aatagcatcc cgacttcatc ggaaatcgct tcacgctcca tttttgtcag taatgaatcc
      241 ggatatatat tcatacccca tagcaacctt tttcaagatc gagagctgcg ccagtttccc
      301 atcaccggtt atctgcaaga tgtgaattat aatacttggg tcacgacagc gggagccgga
      361 gcttggtttt caaataatgg ttcatccatc ttatcgccac tcaattatgg actcaatgct
      421 tcgtcggtca aagcgatggc ctttgatggt gctgatatgt ggatcggtgc gacggccaat
      481 actctaccgg gacaatatcc gagcgaacta tccggactta cccaatggaa tcagaaaaac
      541 gatcacttca cgtattattt cagcagctta catgataacc ttgctctgaa tgaaatcacg
      601 gatattgaag ccgatgaaaa ttatatttgg gttggtacga caagtggttt gaattatttg
      661 aataaaaaaa gcaatcaatg gactatcgct aagacataca gcggtcttaa ggacgatcat
      721 atcatagaaa ttgttccgtt tcaaaaaggt attatagtag cgactgaaaa cgggctgcat
      781 cgcagcatac tcaccaaaaa aggaattcaa ttccagccgc tcgaaattcc tgacttatgg
      841 aaccggcatg ttttaaaaat attgccctcc ggaaacagca tctggcttgg cacggataac
      901 ggtatttatc gtttagattc cagttttact attcaaaacc actatacggc ccttggttat
      961 gacataccgc tcaattcatt ggtcaaccaa gtcgtaccgg ctataaccga aatggcgaac
     1021 aacttatttt tcatatcaac ccatagtgta ttacggtttg acaaacataa gaaggaatgg
     1081 gaatccatgc cgatcagcgg ttcgtatctt caatccggcg tatatgacgc tatcgccgat
     1141 gaagataatc tttggatcgg tttagcggat ggcgctctgc aatatgattt tgaacttcag
     1201 cgctgggctt attacaataa aaaagacggc atcggcggct attcggtgta taaaatttta
     1261 cccgacggtg actatgtttg gtttgccacc gaaaacggat tgacacagtt ttactggaat
     1321 gccgagcatt tacgaagtca aaagtagtta tttgcccgta agttgacttt ccgaatctat
     1381 caaatatcct cccgttttta cgatcacttc tcctgccgta atgcctgaaa gtacagtata
     1441 ttgatcgcca actttttcac caagcattat ttcgcgctgc tcaaagtgat ggggcgatgt
     1501 catcacccat gtcaaagcac gtttgccggt aagaagcact gccgtttcag gtataaccgg
     1561 taattccggt aagggggaat caaatatcat ttgtccgtac atttttggcc gcaaacgata
     1621 gtctttatta ttgatttcga tacgtacttt gattgtgcgg gatacgggct ctatcacggg
     1681 gtaaataagt gtgatggtac cggtaaattt ttcatcggga tatgtatcca accgaattgt
     1741 tacagtgcta tgcaatttta aaaaacgggc ttcggattca aatatatccg aaactcccca
     1801 aatcaaactc aggtcggcca tatcatacaa aatcgttcct tcattgacat aggcgccttc
     1861 ggtgattttt ttttcgatca ctgtaccact ataaggggaa tgataaataa agttttgttt
     1921 aatttcgcgc gtgcgccgaa gcgtttccaa ctgatccgaa gtaactccca ataatcgaag
     1981 ctttgtctca gccgctgaaa gcatctgatt tgtataggat tgtccttctt tattatttaa
     2041 cagaacaaca aaatcatttt gcgcctgaac gagctccggg ctatatattt caaacagcgc
     2101 ctgaccggct tcgatacgat cccctgtttt ggctacgtat aacttttcaa tgcggccgct
     2161 aaagcgtgct gcaatggttt tacgtagatc ttcagcaaat tctaacgtcg taaaacctgt
     2221 aatcgtttta tttagtttgg acgtacggac tttcatcgtt gtaacttggg ccgaacgcat
     2281 ttgatcagga ttaagaaata aaccatggtg cattgtatcg gcagcggatt ccacacgcga
     2341 ttttttcact aaatccattt gacaaatagg acaaactccc ggcttgtcgg atgtcacttc
     2401 tttgtgcata ggacaataat acaaatcggc atgatcggaa tgagacttgt tattctcttt
     2461 cgatgtgcat ccgctgatcg tgagcgacca tatgctgagt gcaatgaata tgtagggcgt
     2521 catataattt tctttcgttt taactttctt tgatccaatg agccattttg gactttttta
     2581 gtgttccttt gcttaaagct ctttctttca taatagcgaa taaaatcggt gttacgatca
     2641 aaacgtgtat cgccgatgtg atcaaccctc caatcattgg cgctgtcagc ggcttcatca
     2701 catccgcacc ggttcccgtt gaccacatta tcggcaccaa cccaatcaaa gccgtaccga
     2761 ccgtcatcaa tttaggtcgc aatcgcaata cactgccttc aatcgttgct tcataaatat
     2821 ccttttgagt tataactcca tttttggatt gtaaacgacg atccaaagcc tcatggagat
     2881 aaactaccat gataacaccc gtttctgtag caataccata taacgctatg aaaccaaccc
     2941 atacggctac tgaaaaatta taaccgagta taaaattcat atatacaccg ccgatcaaag
     3001 caaacgggac cgaaagcatg acaacaagag cttctatggg atctcgcacc gtaaaataga
     3061 gtaataaaaa aatcaaaata aaaacgatcg ggataataat catcaatgta cgctgtgcgc
     3121 gaagcttatg ttcgtactgc ccgctccact gatacgtata accttcaggt agctttagtt
     3181 cggattgtat caaaactttt gcatcttcta ctacatgatc caaatcgcgg cccgctgtat
     3241 tcatatagac tacggaacga agcataccgt tttcactgtt aaccattgta ggtccctgat
     3301 tgactttgac ttcggctaac tgacccaacg gtaaaaaaat aacgccttca ttaacatctg
     3361 tgagtagtga ttgacgtatc ggttctgata ctactccggt aatttgcgtc atcgaagccg
     3421 gcattgatgt ttttgtgctt tcatacgaga gattttcaat tggtgtggct gaagccatcg
     3481 tattgcgtgg ccttatggga atccacaatt gttttaatgc ctcggtatca ttgcgccact
     3541 cgcgtccaaa tcgagcgcgt atgggaaaac gcattcgacc atccaatact gtgccgatat
     3601 tttgtccgcc gatcgccatt tctataacct cttgcaaatc tccgtttgta aatccaatac
     3661 gttgtgcggc ttcaggtttt atggtaatat ccaggtaata tccattctgt cctcgttcgg
     3721 ccactacatc actcgcgcca tggacatttt taagaatttt ttcagcttcg atggcataat
     3781 actccagcgt atcggcatta ttacccaata ttttaaaacc cacgtcggta cgtacacccg
     3841 tagcgagcat gttgatacga ttaacaatag gttgtgtcca tccgttgcgg acgcccggta
     3901 tctgtagttt agaatctaat tcctgaatga tatctttttt tgtaataccg ggacgccaat
     3961 cttttttgtc ttttaacaaa atgatcgttt cgatcatact aatcggcgca ttatcggtgg
     4021 ccgtttcagc acgaccggcc ttacctaaga catgtgccac ttcaggtacg gctttgatca
     4081 atcgatcctg tatcgtcaga attcgattaa cttccgtaag cgaggcattg ggcagtgtga
     4141 ccggcatgta caagatcgag ccttcatcca aaggcggcat aaattccgat cccatattta
     4201 cgatcatcgg tatcgtaata ataagcgcga tgacgttaag tgataaagta attttacgat
     4261 accgtaaaac ccagtgcatg actggctcgt acagtttgat aaaaaaatgc gttacaggat
     4321 tttccttttc gggtcggaat tttccgcgca tcaaaaatgt cataagtacc ggtatcagtg
     4381 tcaaaacaac agaggccgac gccagcataa caaaggtttt agtaaaagcg agcggagcaa
     4441 aaagtttacc ctcttgaccg gtaagcataa acaccggcaa aaaagaaaca aggatgatca
     4501 gttcggaaaa aaatatggcg cgcccgactt gtttagccga ctgtagagca atggcctgat
     4561 aatcctcggt tagcagcttt cctttagttt ccagtgcctg ggcgatgtta cgataggcat
     4621 tttccaccat cacgatggat gcatccacta tcacgccgat ggcgagtatg atgcccccca
     4681 gactcataat attggcatgt atatcaaaaa tgcgcatcaa aataaatgac agcagaatgg
     4741 atacagggat ttcgatgata atacgaagta tgcttcgtgc atgaagcaaa aacacggcta
     4801 ccataagcga gaccgtcaag gcggcttcga ataaggcccg attaagcgtt tcgattgatt
     4861 gctcgataag cacactgcga tcatacgatg gtttgatgac aacgccatgc ggcaatcccg
     4921 gtgcgatttc atttattttt ttctttatac gatcaatcac ggctttcgcg ttttcgccgc
     4981 tgcgcatgac tacgatcccg ccgacggctt cgccttcacc gtccttttcc aatgagcctc
     5041 ttcgaatatc accaccgatc tgcacatcag ctatttgccc aagcaaaaca ggtatcccgc
     5101 tgccattatt tcgtaatacg agattacgaa tatcgctctc cgttcgaata tacccttgtc
     5161 cgcgtacaaa atattctgcg ctgctccatt cgataatctt gccgcctact tcattattat
     5221 tacgttgaag cgcttgggta acatccatta tatctattcc aaacgcccgc attcgtaccg
     5281 gattcaagtc tatctgatat tgtttgacat gcccccctat acttgccact tcggcgaccc
     5341 cttcaacgga ggaaagttga tacctgatat accaatcttg tatggatcgc aacgttccta
     5401 aatcttgttc gccgccctcg accgtatacc agtaaatatg accgataccg gtgccgtccg
     5461 gccccaatgt aggtatcgca tctttaggca acgatccgcg aaccgtgctc aaacgctcct
     5521 ggacacgcga acgtgcaaaa tagatatccg tattatcatc aaaaatcaca aacacaaacg
     5581 acataccgaa catggaactg gcgcgtacag ccttgacgtg agcaagccct tggagtgctg
     5641 taaccagagg aaatgtcaat tgctcttcga caacttgcgg cgagcgaccc atccattccg
     5701 tatagacgat aacttgattt tcagataaat ccggaattgc gtcaacgctc atttccctaa
     5761 atgaataaat gccgtataac gaaatggcta cataagctgt aagtaccatc caacgatggc
     5821 gggcgctcca atcaatgata ttttcaatca tgatgcgcct cacttttcat gttagacgta
     5881 cctatggctt tataaaaatc agcttcggcc attttactac cggccaatgc catgaccaaa
     5941 tcatctttcg tcatataaat catccgcaac atagacaaca acattgcggt atcagctttg
     6001 cctgtctgaa aagaaatcat catagattgg taagccgctt cataatgcgg gagaatccgt
     6061 tgttcgattt gttcggcctg acggcgcgat gattttatcc tttcgatggc cgatcgcgct
     6121 tcagaagtaa tcatgatctt catgttttct atttccatgg cgccatgttt tatatgcatg
     6181 tccaattcct tgatccgtgc atccagtgac cctcttgacc aaggtgcaaa gggaatatta
     6241 gcgctgagca tcagagacca ataatttttt cccatgctca tatctttata catgcctctt
     6301 accatgatat ccggaaacca ttcgcgatgt gccgcttttt tttcagcagt aaacatcgcg
     6361 atttcatgat tcatcccctg aatttcaggt tttttaggca gtgtattttg tatcaatgta
     6421 tccagtacta cggaaaaatt attcgtccag tccacaggta ttgaatccgg ccatcgaatt
     6481 tcattatttc ccaagactcc atttaacatg gattccgtaa cccgacgatc tttctccaac
     6541 tcgaaagcct tattctctaa agctaatcgc tcattttcga tttgtaatat atcggcttgt
     6601 gttatttgcc cgacttcata gcgtttcaat gataactgta ataattgctc caatgtacgt
     6661 tgcgcattag cgttattacg tctacgttcg ttaatcatac ataactcaaa aaaagccaca
     6721 cgtgcatcac gctctatgtt taattgggcg cttgcaatca ttgtcttttg aatttctgct
     6781 gtataatgtg cggcctccga catggcctcg agtttaccgg gccaaggtat catttgttgt
     6841 ataaaataat ctgtttccat attccctttg accggtatag gaaaattgct cgccggcatt
     6901 tgaaataatt cgaatcccac atccggcgtc ggccatgatt ttttttgttt tgcttgaaaa
     6961 cgatacgctt cataattgtg ctgtatcgcg tgaattttgg gatgatgttg tatggcctcg
     7021 cgaataagac tgtctatcaa tacctgcgtg tgaacctgcg gtatctccaa acttgcgaga
     7081 ccgatgatta gcgctatttt ttttaacgat ccggacataa cttttcctca ataaattgaa
     7141 taccggctaa atactatgat ttagcataga ttcagtttaa tcggaaaatt ctaaatgcgg
     7201 aatgtactaa ggagagcgtt ggtttctgag gccgtattgg caggtggatg ctgatgggaa
     7261 aaatcgaaaa aagaattctt attgtatgct tgatcaaagg cggtttgaaa tacaaaattc
     7321 agtaatgcat gcgtttgctg caccgagttg gttttgagtg tttctatagg taacgaaaca
     7381 ccacgctccg gcaaaacgct catcattccc ggacaatggc atgatttata ggcagcgatt
     7441 tgaagcgaat tgcttgtacc ggccatagtc gtagaagact ggcaacaaga catcgaatta
     7501 gtttttgttg aatagcatgt cccggtaatg cggacattcg ctaaacaacc ggtcacccaa
     7561 accagcgcca gaagaagaga taaaaccttt tgtaggcttt gattattcat atgttaaaaa
     7621 taccccggaa aacctgcgga atcaagcaat ttccaatggg aatcatgaat attgtttttt
     7681 acgatttgta ccacattcat atcggaccac tgaggatctt tttcagcaca taaacgcgta
     7741 aatgatttga tccattcgga aacgccgaca atatcatctc cgttcagctg aatcggtttt
     7801 aatgccgttg ttttacctcg ctgatctacc acaaaatatc cgagagtatt ccatttattc
     7861 acatgtgcga gttgcgcacg acgaaaataa tttcctactt tcgcttcgcc catagattgc
     7921 cctacgtgca tggtagccgc atttaatgcc gcaattactg catcggccgg tatggatacc
     7981 gctttactcc ctaaaggagc aggcgccatg cctccttctt tggatgacat ggttgactta
     8041 gcatccgaac gcaacgattt ggtcagcgca tctaaggtta acaattgttg attgatttca
     8101 aatgtaccgt tttgccatga catgagttcg tcaatggcgg catcatcttt tttatcgtca
     8161 agcgatactt ccagcagctc gccttttctg tacatcaatt tcccgcgacg gttttcgtgc
     8221 gttacgatca attcacccgt cagcgaattg gcttcacaaa aagcaatcac atcaatcaaa
     8281 ggtttttctt ctaattttcc aacgggctta ttttcacgac gcgtatccag atctgctttc
     8341 accgcatggc cttgctgagg ttgaggtttg gtctggtgac gtgcttcgat tttggagaga
     8401 accatttgta cgcgcgcaat gacctctttg attcgaatcg gcttggaaat atagtcttca
     8461 gcgcccaatt gaaggccttc gataatatga tcgttatccg tagcggccgt caaaaaaata
     8521 accggtatct gattataata cgggtctttc tttaaacttt gcaaaagctg aaatccgttc
     8581 atcatcggca taaaaatatc gctgagtatc aaatctacat tctgattctc tacaacctta
     8641 agcgcttcag aaccgtcgga agcggaaaag gttttgaacc cgttttgttg aagggccatt
     8701 tcgcaaataa gacggatgct ctcttcgtcg tcaaccacaa ggatgcgttt cttttcagtc
     8761 attggttttc catatatcgt tgggtgattt ccaataatct tttggtttct acgggttgct
     8821 caatgatttc ataaatacgc gcctggattt tgagcgccat gatatgatgc ggctgttctt
     8881 ttaataccga tgcggtgatt tccaacgcat ctttgtataa ctggtaccgc atatagatgg
     8941 tcgcggtcaa cagctttttt aatacagtat cttccgttaa ctcaatagca tcgttacttt
     9001 cccgtaattc attctcaaat tcagtgcgtt cttctttatt catcacataa aaccaaacac
     9061 tctctgttgc ctcattgata ttctcattca ccgcttccgt aaaccaaaaa tatttaatgc
     9121 cgcgctccat attcatttcg ttacgaaacg tgaatgaatt accgagcacc gtctcttcaa
     9181 aagagtaatc attttcatac gatcgtaaat ggacaacaaa ttcagacgca ccggttattt
     9241 tatcagggat atgacgccat atcaattgcg ctggaatatc tataagccgt gtattacgtg
     9301 gagttattgc agataaagaa acctctgcat cacttcgata aatttgtttg gaataatgag
     9361 actgagcttt aaaaaaccac gat
//
LOCUS       DAOQGF010000120         9204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3537_length_9204_cov_6.057055, whole genome shotgun sequence.
ACCESSION   DAOQGF010000120 DAOQGF010000000
VERSION     DAOQGF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_9204_cov_6.057055"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK937_13260"
     CDS             <1..354
                     /locus_tag="PK937_13260"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH33588.1"
                     /translation="KIENPCTPEMIARARHLIRQIVVDDGILKYITEVVRKTRETSGI
                     MVGASPRATINLLLAAKTQAALANRTFVIPDDVKDLAPAVLRHRLVLQPETEMEGLRP
                     DDMIRDVLNTVPVPR"
     gene            375..1538
                     /gene="nhaA"
                     /locus_tag="PK937_13265"
     CDS             375..1538
                     /gene="nhaA"
                     /locus_tag="PK937_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNH33589.1"
                     /translation="MKKILNPIQEFIHYEQSSGIILIFCTIVSLALANTPLNEPIYQF
                     WHTRIGFATENIDLHKTILHWVNDGLMAVFFLLVGLEIKREIVSGELSSLRRASLPLF
                     GAVGGMLVPALIYVSFNQGTIYENGWGVPMATDIAFALAILGLLGSRIPVSIKVFLTA
                     LAIIDDLGAIIVIALFYSSTLFLNYKAAALVTVLVLILINRLGIRSLAIYLGLGILLW
                     YFVLKSGIHATIAGVILAMTIPYSKDETSSPLLALEHALHKPVQFFIMPIFALANTLI
                     VMDASVWVALFSTLSIGIFTGLFFGKVCGILIFCWIGTKLNLAALPDGVTWHHLIGTG
                     FLAGIGFTMSIFIAVLAFENQEGIQTTAKAAILAASIVSGLTGFMILRRTIKT"
     gene            complement(1535..2245)
                     /locus_tag="PK937_13270"
     CDS             complement(1535..2245)
                     /locus_tag="PK937_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNH33590.1"
                     /translation="MPTVFHKAESRGFADHGWLRSYHTFSFAGYHDPKRMNFGLLRVL
                     NDDTVRPGSGFGEHPHDNMEIISIPLKGTLAHADSTGSEKAIRTGDVQIMSAGSGLTH
                     SEYNHSASAPVNFLQLWIYPKRRNIEPRYDQKTFDLASRKNQWVTVVAPDENAESLWI
                     NQDAVLSLAVVERGEILYTPRFKENGIYIFMIDGNAQYESQLLGRRDAIGIHPAGDCN
                     LIIRQPSHILLIEVPMNF"
     gene            complement(2259..2726)
                     /locus_tag="PK937_13275"
     CDS             complement(2259..2726)
                     /locus_tag="PK937_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNH33591.1"
                     /translation="MKFSTQEEYGLRCLLQIARSTNKNGMTIPQISQAEGLSEANVAK
                     MLRILRMGGIIESARGHEGGYRLARTADQIRIGDVLTVLGGRLFDHEFCEKHAGEENI
                     CSHGTDCTLRHVWQTVQTAVDDVLMRLTLQDLVHPAQKPITEIKIPSTLVSTT"
     gene            complement(2742..3071)
                     /locus_tag="PK937_13280"
     CDS             complement(2742..3071)
                     /locus_tag="PK937_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNH33592.1"
                     /translation="MIEETKLGPTGMEIQVIEAIRTCYDPEIPVNIYDLGLVYEVKVD
                     AAGVASILMTLTSPSCPAAESLPPEVESKARAVPGITDVKLELTWEPPWDPTRMTEAA
                     RLELGMM"
     gene            complement(3075..3530)
                     /locus_tag="PK937_13285"
     CDS             complement(3075..3530)
                     /locus_tag="PK937_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014148483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNH33593.1"
                     /translation="MMSDLRELYQEVILDHNKNPRNFRVMENADIVKEGFNPLCGDHL
                     HLYLKLDGDVIQDVSFEGNGCAISKSSASLMTTVLKGKTKSDAEILFKRFHDLVTADM
                     STTVDEESLGKLAVFAGVREFPARVKCASLAWHTMHAAIANDTTIVSTE"
     gene            complement(3527..4777)
                     /locus_tag="PK937_13290"
     CDS             complement(3527..4777)
                     /locus_tag="PK937_13290"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNH33594.1"
                     /translation="MNSTEQLKPFSDAEVAAMRRDFPILSEHVHGKPLVYLDNGATGQ
                     KPKQVIDAIARYYSRENSNIHRGVHYLSAQATIAYENARKKIQKLIHAPSDNEIIFLR
                     GTTEAINLVAQSYGRTYIQRDDEVLISAMEHHANIVPWQMLCEQTGAKLRVIPMNERG
                     ELRMDAYQTMLTEKTKIVAIVHVSNSLGTINPVKEMIALAHAKNIPVIVDGAQAVQHM
                     PVDVKDLDCDFYAFSGHKMFGPTGVGILYGKKKWLDAMPPYQGGGDMIRSVTFEKTTY
                     NDVPHKFEAGTPHIAGGIALGDTVDYLQGIGLERIAAYEKVLLDYATEKLSAVSGLHI
                     IGTAHNKAGVISFILDGVHPHDVGTMLDQEGIAIRTGHHCTQPVMQFFKVPATSRVSL
                     AFYNTHDEIDTLVKAIHKTMEMFR"
     gene            complement(4778..6100)
                     /gene="sufD"
                     /locus_tag="PK937_13295"
     CDS             complement(4778..6100)
                     /gene="sufD"
                     /locus_tag="PK937_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNH33595.1"
                     /translation="MINQTEAKNWFQNQFARFESALNGQKSGVTHALRRDAMARFQAS
                     EFPTMRHEEWKYTNLESLFTHGLHVASPTDSASHALDIRNDLTLDTEAYTVVFVNGYY
                     DAKRSTINAPEKGLQIRLLSQCSDEEKAGILRALAAETDESVFNTMHMALVSDGVVID
                     VAGGAVIGKPVHIQYFTVAEESAPMTQPLTLIRVEKNAQCTVIEDYKTVGENPFLTNA
                     RTEIIVAENAVVDHARIQNESVKGYHISLTRLRQARSSNFMTTHITFGAALSRNLTIA
                     HLDGEGIESTLNGLYMPKGRQHTDNRTIIHHAKPHCNSHELYKGIMDDHATAVFNGKI
                     FVHPDAQKTDAKQSNNNLLLSKDATINTKPQLEIFADDVKCTHGATIGRLEEKGMFYL
                     RSRGIGYDEARSIMTLAFAEDIVSRIRHTALRKRLDTMLHERLNMKFE"
     gene            complement(6100..6858)
                     /gene="sufC"
                     /locus_tag="PK937_13300"
     CDS             complement(6100..6858)
                     /gene="sufC"
                     /locus_tag="PK937_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNH33596.1"
                     /translation="MLEIKNLHATIDGNPILKGINLSVKAGEVHAIMGPNGSGKSTLA
                     HILAGKEDYEITQGEIIFNGKNLTELDPEDRAAEGVFLAFQYPVEIPGVNNTYFLKAA
                     LNAIRKYRGEEPLDAMDFLSLVKQKMKLVEMDEDLLKRPVNEGFSGGEKKRNEIFQLA
                     VLNPKLAILDETDSGLDIDALKIVSNGVNKFRSGDNATIVVTHYQRLLNYIVPDFVHV
                     LMDGRIVKSGGKELALTLEDRGYDWIKEHDGVVA"
     gene            complement(6898..7935)
                     /locus_tag="PK937_13305"
     CDS             complement(6898..7935)
                     /locus_tag="PK937_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33597.1"
                     /translation="MSDFFNIYFDWFLGYLFLVLPGIVVFTLMIVKLFKIVTKRNRWI
                     TFFALALLIHTIFFVYYDHQLSRMGEVTRIISADDVLMVEGVLTNTGYRSSRGSASGF
                     REDPATTTQAQKIFVFDKKSGNILQRIEDYQVAHYAERRAFLVPTIATKSYAIFDTRV
                     SQFDRFFISSDLRSDMPELANRIDKITYDRSWMLKPDMSGSGWDEAVPYFELTLSDGS
                     GLYYDVLTGETHPRDKKQTSWTGEKRPALFTEYADFTIDSSSTGVVRALKKRASENGF
                     VLFAVRADGQKKWTVTAEQIHPEIKDDRFFFSENPGSYLVEDGKLYFFTYRFAVALDT
                     ENGHIIWQQSI"
     gene            complement(7938..>9204)
                     /gene="sufB"
                     /locus_tag="PK937_13310"
     CDS             complement(7938..>9204)
                     /gene="sufB"
                     /locus_tag="PK937_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNH33598.1"
                     /translation="WLKMCEEGEPTWQNVHVAKVDYQDIIYYSAPKQKKKSADEVDPE
                     LLKTYEKLGIPLNERLALAGVAVDAVFDSVSVATTFKTKLKELGIIFCSFSEAVQEHP
                     ELIKQYIGSVVPPTDNFYSALNSAVFTDGSFCYIPKGVRCPMELSTYFRINAASTGQF
                     ERTLIVADEGSYVSYLEGCTAPMRDENQLHAAVVEIYAHTNAQVKYSTIQNWYPGDKD
                     GKGGIYNFVTKRGLCAGDNSKISWTQVETGSAITWKYPSCILKGDNSVGEFYSVALTA
                     KRQQADTGTKMIHLGKNTRSTIVSKGISAGHGQNTYRGLVRVGKNAENARNFSQCDSM
                     LLGDQCGAHTFPYIEVKNNTAQVEHEASTSKIGEDQIFYCNQRGISTEDAVSMIVNGF
                     CKEVFKELPMEFAVEAQKLLGVSLEGSVG"
BASE COUNT         2251 a         2313 c         2110 g         2530 t
ORIGIN      
        1 aaaatagaaa acccgtgtac accggaaatg atcgcgcggg cacgtcattt gatacgacag
       61 atcgtggtgg atgacggaat actaaaatac atcacagagg ttgttcgcaa aacgcgcgaa
      121 acttccggca tcatggtcgg cgcaagccca cgcgctacga tcaacttgct tttggccgcg
      181 aaaacgcagg ccgcgttggc taatcgcacc ttcgtcatac cggatgacgt caaagatctg
      241 gcccctgccg tactgcgtca tcgccttgtg ctacaaccgg aaaccgaaat ggaaggtctg
      301 cgccccgatg acatgattcg ggacgtactc aatactgttc ctgtacctcg ctaatgaaag
      361 taaaatctga caacatgaaa aaaatattaa atcccattca ggaatttatt cactacgaac
      421 agtcgtcggg aataatcctt attttttgta cgattgtgtc tttggcttta gccaatacgc
      481 ctctcaacga acccatctat caattctggc acacacgtat tggatttgct acagaaaata
      541 ttgatctcca taaaaccatt ctgcactggg tcaatgacgg attgatggcg gtgttttttc
      601 tgctggtagg tcttgaaatc aaacgcgaaa tcgtgagcgg cgagctttcc tctctgcgtc
      661 gtgccagtct gccgctcttt ggcgctgtcg gtggaatgct tgtaccggca ttaatttatg
      721 taagcttcaa tcagggtacg atatatgaaa acggttgggg tgttccgatg gccactgata
      781 tcgccttcgc gttagccata ctcggattat tgggttctcg catacctgtg tcgataaaag
      841 ttttcctcac ggcgcttgcg ataatcgatg accttggcgc gatcatagtg atcgccctgt
      901 tttactcatc tacattattt ttaaactata aagcggcggc tttagtgact gtacttgttt
      961 tgattttgat aaatcgatta ggcatacgct ctcttgcaat ctatcttgga ttgggaattc
     1021 tgctttggta cttcgtactc aagtcaggta ttcatgcaac catcgccgga gtcattttag
     1081 ccatgaccat accgtattcc aaagatgaga catcttcccc gttacttgcg ctggagcacg
     1141 cactgcacaa accggttcag ttttttatca tgcctatttt tgcactggct aacaccttaa
     1201 ttgtcatgga tgcatcggta tgggtcgcac ttttttcaac gctaagcatc ggtattttta
     1261 caggtttgtt ctttggcaaa gtttgcggca ttctgatatt ttgctggatc ggcacaaaac
     1321 ttaatctggc tgcattgccc gacggtgtca cgtggcatca cttgataggt acgggttttc
     1381 tggcgggtat cggttttaca atgtctatat ttatcgcagt attagctttc gagaatcagg
     1441 aaggcataca aactacagcg aaagcggcta ttttggcggc atctatcgtt tcagggctga
     1501 ccggttttat gatactgcgc cgaacaataa aaacctaaaa attcatcggc acttcgataa
     1561 gcaatatatg cgacggttgc cgtattatta aattacaatc gccggccggg tgtatcccga
     1621 tcgcatcacg acgaccaaga agttgactct catattgcgc attgccgtca atcataaaaa
     1681 tataaatacc gttttctttg aaacgcggcg tgtataaaat ttcgccgcgt tcaaccacgg
     1741 ctaacgataa tacagcatcc tgattgatcc agagcgattc cgcattctcg tccggcgcca
     1801 cgacagtaac ccattgattt tttcgtgaag caaggtcaaa cgttttttga tcatagcgcg
     1861 gctcgatatt tctgcgtttg gggtaaatcc acaattgaag gaaattaaca ggcgcactgg
     1921 cgctgtgatt gtattccgaa tgcgtcaaac ctgaaccggc ggacatgatt tgtacatcgc
     1981 ccgtacgaat agctttttcg ctgccggtac tgtcggcatg cgcgagcgta cccttcagcg
     2041 ggatcgaaat aatttccata ttatcgtgcg ggtgctcacc gaatcccgat cccggacgca
     2101 ccgtgtcatc attgagtaca cgcagcaggc caaaattcat ccgcttcggg tcatgataac
     2161 ccgcaaaact aaatgtatga tacgaacgga gccagccgtg atcggcaaac ccgcgcgatt
     2221 ctgctttgtg aaacaccgtc ggcataagga atacttattt aagtggtaga tacgagcgtg
     2281 gaaggtattt taatttccgt aatcggtttt tgtgcaggat gcacaagatc ttgaagggtc
     2341 agtcgcatca acacatcgtc caccgccgtt tgtacggttt gccaaacatg gcggagcgtg
     2401 cagtccgtac cgtgcgaaca aatattttct tcccctgcat gtttttcaca aaactcatga
     2461 tcaaacaaac gcccacccaa taccgtaagc acgtcaccga tacgaatctg atcggccgtt
     2521 cgcgctaaac gatagccgcc ttcatgaccc cgcgcacttt cgataatacc gcccatgcgc
     2581 aggatacgta acattttggc aacgttggct tccgaaagac cttccgcctg actgatctgc
     2641 ggaatcgtca taccgttttt attcgtactg cgggcgatct gcaaaaggca tcgcaatccg
     2701 tattcttctt gtgtactgaa cttcatattt tttccgtatc gttacatcat acccagttcg
     2761 aggcgtgctg cttcggtcat gcgggtcggg tcccagggcg gttcccacgt caattccaat
     2821 tttacatccg tgataccggg cacggcgcgt gctttggatt cgacctccgg aggcaaactt
     2881 tctgccgcag ggcaactcgg cgaagtcagc gtcatcagaa tagacgccac tccggccgca
     2941 tccactttca cttcgtacac taaaccgaga tcataaatat tcaccggtat ctcgggatca
     3001 taacacgtac gaatcgcttc gatcacttgt atttccatgc cggtcgggcc gagtttggtt
     3061 tcttcgatca tggttcactc cgtagaaacg atggtcgtat cattggcaat cgcagcatgc
     3121 atcgtgtgcc aggccaaact cgcacacttc acgcgggcgg gaaattcgcg tacgccggca
     3181 aatacggcta acttacccaa cgattcttca tccacggtcg tactcatatc ggctgttacc
     3241 aaatcatgaa accgtttaaa taaaatttcg gcatccgact tggttttgcc tttgagcaca
     3301 gtggtcatca gggatgcgga tgatttggaa atagcacagc catttccctc gaagcttacg
     3361 tcttggatca catcaccatc cagcttgaga taaagatgca aatggtcacc acacaacgga
     3421 ttaaaacctt ctttgacaat atccgcattt tccataacgc gaaaatttcg ggggttctta
     3481 ttatgatcaa gaatcacttc ctgatacaat tcacgcaaat ccgacatcag cgaaacatct
     3541 ccattgtttt gtgaatggct ttcaccagcg tatctatttc atcgtgcgta ttataaaatg
     3601 ccagcgaaac gcgcgacgtc gcaggtactt taaaaaactg cattaccggc tgcgtacaat
     3661 gatgtccggt gcgtatggct attccttcct gatccagcat cgtacctacg tcgtgcggat
     3721 gtacgccgtc caatataaac gaaatcacgc cggctttatt gtgtgccgta ccgataatat
     3781 gcaaacccga aacggcgctc agtttttccg ttgcatagtc cagcaatact ttttcatacg
     3841 ctgcgatacg ctccaaaccg ataccttgca aataatcaac ggtatcacct aaagcaatac
     3901 cacctgcgat atgcggcgtg cctgcttcaa atttgtgcgg cacgtcgttg tacgttgttt
     3961 tttcaaacgt caccgaacga atcatatccc ctccgccttg atacggcggc atggcgtcca
     4021 gccacttctt ttttccgtag agaataccca cgccggtcgg accgaacatt ttatgacctg
     4081 aaaacgcata aaaatcacaa tccaaatctt tcacatccac gggcatgtgt tgcaccgctt
     4141 gggcgccatc tacgatcacg ggaatatttt tggcatgtgc aagtgctatc atttctttga
     4201 caggattgat tgtaccgagc gaattggaca catgcacgat cgcaacgatt ttcgtttttt
     4261 cggtgagcat cgtttgatac gcgtccatgc gcagttcgcc acgttcattc atcggaatga
     4321 cacgcagttt ggcaccggtc tgctcgcaga gcatctgcca gggtacgata ttggcatgat
     4381 gttccatcgc cgaaatgagc acttcatcat cacgttggat atacgttcgc ccgtaggatt
     4441 gcgctaccag attgatggct tcggtcgtgc cccgtaaaaa tataatctca ttgtcggagg
     4501 gcgcatggat taatttttga attttcttcc gcgcgttttc gtatgcgatc gttgcctgcg
     4561 cactcaaata atgcacgccg cgatgaatat tggaattttc acggctataa taacgcgcaa
     4621 tggcatcaat cacttgcttc ggtttttgtc cggttgcacc gttatctaaa taaaccagcg
     4681 gctttccgtg aacgtgttcg ctcaatatgg gaaaatcccg tcgcatagcg gccacctccg
     4741 catcggagaa tggttttaat tgttcggtac tgttcatcta ttcaaacttc atgttaagcc
     4801 gttcgtgcaa catcgtatcc aggcgtttac gcagtgccgt atgacgaatg cgcgacacga
     4861 tatcttcagc aaacgccaaa gtcatgatgc tgcgcgcttc gtcatagccg ataccgcgcg
     4921 agcgaagata aaacatccct ttttcttcca gtctcccgat cgtcgcgccg tgcgtacact
     4981 tcacatcgtc ggcaaatatc tcaagctgcg gtttggtatt gatcgtcgcg tctttggata
     5041 agagcaaatt attattcgat tgtttagcat ccgttttttg cgcatcggga tgaacaaata
     5101 ttttgccgtt gaaaaccgcc gtcgcatggt catccataat gcctttgtac aattcgtggc
     5161 tgttacagtg cggcttggca tgatgaatga tggtgcggtt atcggtatgc tgacgacctt
     5221 tgggcatgta taatccgtta agcgtagatt cgatgccttc gccatccaaa tgcgcgatcg
     5281 tcagattgcg ggacaacgcc gcgccgaacg taatatgggt cgtcataaaa ttactgctgc
     5341 gcgcttgccg caggcgtgtc aggctgatat ggtatccttt gacggattca ttttgaatgc
     5401 gcgcatgatc cactacggca ttttcggcta cgatgatttc agtccgcgca ttggtgagaa
     5461 acgggttctc cccgacggtt ttgtaatctt cgatcaccgt acattgggcg tttttttcca
     5521 cacgtatgag cgtcaatggc tgcgtcatcg gagccgattc ttcggcaacg gtgaagtatt
     5581 ggatatgaac cggtttaccg atcactgcac caccggccac atctatcacc acaccatcgg
     5641 ataccagcgc catgtgcatg gtattaaaca ctgattcatc cgtttccgcc gccagcgcac
     5701 gcaaaatgcc ggccttttct tcatcgctgc attgcgacag caatcgaatt tgtaaccctt
     5761 tttccggtgc gtttatcgtc gagcgtttgg catcgtaata tccattcaca aaaacaaccg
     5821 tgtacgcctc tgtatccagt gtcaagtcat tccgtatgtc aagcgcatgc gacgcgctat
     5881 ccgtcggtga agcgacgtgc agtccgtggg taaagaggga ttccagattc gtatatttcc
     5941 attcttcatg acgcatcgtg ggaaattcag acgcctgaaa gcgtgccatc gcatcacgcc
     6001 gcagcgcatg ggtaacgcct gatttttggc cgtttaatgc ggattcaaaa cgtgcaaact
     6061 gattttgaaa ccagttctta gcttcggttt ggttgatcat caggctacca ctccgtcatg
     6121 ttctttgatc cagtcataac cgcgatcttc gagggtgagc gccagctctt tgccaccgga
     6181 cttgacgata cgtccatcca tcagcacgtg cacaaaatcc ggcacgatgt agttcagtaa
     6241 acgctgatag tgcgtcacga cgatggtcgc attatcaccg gagcgaaatt tattcacgcc
     6301 gtttgaaacg attttgagtg cgtcaatgtc gagacctgaa tcggtttcat ccagaatcgc
     6361 taatttcgga ttgagtacgg cgagttgaaa tatctcatta cgttttttct caccgccgga
     6421 aaaaccttca ttcacggggc gcttgagcag gtcttcatcc atttcgacga gcttcatttt
     6481 ctgttttaca aggctcaaaa aatccatcgc gtccaaaggc tcttcgccgc gatatttgcg
     6541 aatggcatta agcgccgctt tgaggaaata cgtattgttg acgccgggaa tttcaaccgg
     6601 atactgaaac gcgagaaata caccttctgc tgcgcgatct tcgggatcaa gttccgtgag
     6661 gttttttccg ttaaaaatga tctcgccttg cgtgatttcg taatcctctt taccggctaa
     6721 aatgtgagcc aaagtgcttt tgccggaacc gttgggtccc atgatcgcat gcacttcgcc
     6781 cgctttgacg gaaagattga ttcctttgag aatcgggtta ccgtcaatgg ttgcgtgtag
     6841 atttttgatt tcgagcatgt cttcttttct ctgctataaa tgatgatatg aataaatcta
     6901 aatactctgc tgccaaatga tgtgcccatt ctccgtatcc agcgcaacgg caaagcggta
     6961 tgtaaaaaaa tacagtttac cgtcttcaac cagatacgaa cccggatttt cgctgaaaaa
     7021 aaaccgatcg tctttgatct caggatggat ctgttcggct gttacggtcc attttttttg
     7081 tccgtccgca cgcacagcaa ataatacaaa gccgttttcg gaagcgcgtt ttttgagcgc
     7141 acgtacgact ccggttgagg acgaatcaat cgtaaaatcg gcatactccg taaacagagc
     7201 cggtcgtttt tcgccggtcc acgaggtttg ttttttatcg cggggatgcg tttcgcccgt
     7261 gagcacatca taataaagcc cggacccatc ggacagggtg agttcaaaat aaggtacggc
     7321 ttcgtcccac ccggagcctg acatatcggg cttgagcatc catgaacggt cgtatgtgat
     7381 cttatctatg cggtttgcca actcgggcat atcggaacgc agatcactcg atataaaaaa
     7441 acgatcaaat tgggatacac gggtatcaaa aattgcgtac gatttagtgg cgatggtcgg
     7501 tactaaaaat gcgcgccgtt cggcataatg cgccacctga taatcctcaa tgcgctgcag
     7561 aatgtttccg ctttttttat caaacacgaa gattttctgc gcctgtgtgg tcgtcgcggg
     7621 gtcttcgcga aatccggacg cgctgccgcg gctgctccgg tatccggtat tggtaagtac
     7681 gccttccacc atcaacacat catccgccga tatgatgcgc gtcacttcac ccatacgcga
     7741 gagctgatgg tcataataca caaaaaaaat cgtatgtatc agtaatgcca acgcaaaaaa
     7801 agtaatccag cggttgcgtt tcgtcacgat cttaaaaagc ttgacgatca taagtgtaaa
     7861 cacgacgatg ccgggtaata ccaaaaagag ataacccaga aaccagtcaa agtatatgtt
     7921 gaaaaaatcg ctcatcctca acctacgctg ccttcgaggc ttacgccgag cagtttctgc
     7981 gcttcgacgg caaactccat cggcagttct ttgaaaactt ctttacaaaa tccgttgacg
     8041 atcatagaca cggcgtcttc ggtagaaata ccgcgctgat tgcagtaaaa aatctggtct
     8101 tcgccgatct tcgacgtgga tgcttcatgt tctacctgag cggtattgtt tttcacttcg
     8161 atatacggga aagtatgcgc gccgcactga tcaccgagca acatcgaatc gcattgggaa
     8221 aaattacgcg cattctctgc gtttttgccc acgcgcacca agccgcgata ggtattttgg
     8281 ccatggcctg cggagatacc tttggaaacg atcgtgctgc gcgtattttt gccgaggtga
     8341 atcatcttcg taccggtatc ggcttgctga cgtttggccg tgagcgcgac ggaataaaat
     8401 tctccgaccg aattatcacc tttcaaaatg caactcggat atttccatgt gatggcggag
     8461 ccggtttcta cctgcgtcca ggagatttta gaattgtcac ccgcgcacaa accgcgtttc
     8521 gtcacaaaat tataaatccc gcctttaccg tccttgtcgc ccgggtacca attttgtatc
     8581 gttgaatatt tgacctgtgc attggtatgc gcatagattt cgacgacggc agcatgcaat
     8641 tggttttcat cgcgcatcgg tgcggtacat ccttcgagat aactgacgta gctgccctcg
     8701 tccgccacga tgagcgtgcg ttcaaactgg cccgtactgg cggcgttgat gcggaaatac
     8761 gtcgaaagtt ccatcgggca ccgtacgcct ttggggatat aacaaaacga accgtcggta
     8821 aataccgcgg aattgagcgc ggagtaaaaa ttatccgtcg gcggtacgac cgaaccgatg
     8881 tattgcttga tcaattcggg atgttcttgc acagcttcag agaatgaaca aaaaatgata
     8941 ccgagttctt tgagtttggt tttgaatgtc gtcgcgacgg atacgctgtc aaatacggca
     9001 tccacagcta ctcccgcgag ggcaaggcgt tcattgagcg gaataccgag cttctcgtac
     9061 gttttcagca attccggatc cacttcgtca gcactttttt tcttttgttt cggcgcgcta
     9121 tagtagatga tatcctgata gtccactttg gcgacatgca cattttgcca tgtaggctcg
     9181 ccttcttcgc acattttgag ccat
//
LOCUS       DAOQGF010000121         9189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3546_length_9189_cov_6.219510, whole genome shotgun sequence.
ACCESSION   DAOQGF010000121 DAOQGF010000000
VERSION     DAOQGF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_9189_cov_6.219510"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PK937_13315"
     CDS             <1..948
                     /locus_tag="PK937_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33599.1"
                     /translation="HEVAWEVELMFFMREKIRNTTHTVSNSKIGLLFLRKGFLDEAIS
                     EFQHTIEHDPDLVEVYNHLGLAYLHRGDFSLAIESIQKGIELRPQFPDLRNNLGFAYG
                     KAKKYSEAITEYDKALAINPHYVVVHLNKAITFLESILNNGDTGSLPTVQDRILTVSS
                     IFKTTLMTNPDFANYRFYTFRFEKILKHLTASAYVQAIDIILEVKESIQKPSLDETIH
                     GFYLKFLFGGAGKDEKVINDYRAQLEQHINENPTYADLHNSLGLVYLIQCRNLFLKAM
                     SQFKKAYEINPSYKTAYKNFRLVQNDGREFLNLLRAILK"
     gene            1308..1679
                     /locus_tag="PK937_13320"
     CDS             1308..1679
                     /locus_tag="PK937_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33600.1"
                     /translation="MKLCNRKTVLVVEDEPGIRFVTSEFLKMSNFQVVTAENGQEAMK
                     IIVDNKIDVVVTDIMMPKVNGLELLKFVRRLKGESFPVVVVSGSDLESLHVIESEHTR
                     TLRKPYGLDRLAKEILSISQN"
     gene            complement(1685..2233)
                     /gene="pyrR"
                     /locus_tag="PK937_13325"
     CDS             complement(1685..2233)
                     /gene="pyrR"
                     /locus_tag="PK937_13325"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNH33601.1"
                     /translation="MNEAEKSIIIARAQEINTMLEDMTRSLISSNPSLESLALIGMRT
                     RGEFIARRLQILVKKFSDIELPMGVLDVSFYRDDTRAKLKQPLVQSTEIRFNINERPL
                     VLVDDVLFTGRSVRAAMDELMDFGRPSRIQLAVLIDRGCRELPIQSDIYGKKITSEIC
                     DKVKVCLEEYDKIDEITLIKGS"
     gene            complement(2245..4356)
                     /locus_tag="PK937_13330"
     CDS             complement(2245..4356)
                     /locus_tag="PK937_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNH33602.1"
                     /translation="MFSFLNAFLLYGLVLASIPILIHLLNRKKARVVPFSSNRFLKAL
                     QKKQIKKLRWQQLLLLIIRAMIVACLVIAFGRPVWKWTATNGNINAHAKTSVVLILDN
                     SLSASSVNQRGQVLERIKNKAMEVISHLQEGDEFVIIDASGLKKASNLNWSSNFSEAR
                     ARLKNIVIENYSFAIDKALHESVSLLAQAKNPNREIYIISDLQKSDRFNTQIPDYGHI
                     KPHLVVIAIDGKPESDLAILKAETENRFFQKSHPIEMVCTIRNSLSTQSGERILHVYA
                     NGKRVGQSVLQVDAKTDTDVRISFPASESGFVSGYAELDDDGFLANNRYYFHYYVPSD
                     IKVLVAGELADKEFIELALKAHDQQAGNIITKSVLFDKLATVDLKKFDVLILVNPGRW
                     DESQTNRVVEFLKEGGGILMIPGTKTDITQFNNQIADRLDVSRLIQMVSLNSENHSFI
                     ELQFDAQPHPLIADIFTDRTSLKNIESPKFYRYFNTDSRHGQNILTFGKSGNFLTEYA
                     IHNGYFMLMTSMPVMTWNDLPIRAFFVPLMHRCLYALYGKENDKNQFVVGDAVRVRIQ
                     RDANDMVRMRNLTGEQSDYIPSLVVQNNIKIAQIDKLDMAGNYSLVDKNNSRVLTLVS
                     ANIPSQENEFAIWESDSLKIFSGTMNVTFLTENESIENYILQSRFGIESWKWFIAVAI
                     ILMFFELALGQMHRLQRNTNK"
     gene            complement(4358..5074)
                     /locus_tag="PK937_13335"
     CDS             complement(4358..5074)
                     /locus_tag="PK937_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH33603.1"
                     /translation="MIASAESKKILVRAQAISKNYTMAKGELTVLKNISIDIHEGEFI
                     VILGPSGAGKSTLLHILGILDKPSSGDVFFENTQVSQLSDVAMARLRNARVGFVFQFH
                     YLLPEFTALENVIMPALIAGKPSNDPSSYERGKNLLKNVGLAERYYHRPSELSGGEQQ
                     RVAFARALMNDPGLILADEPSGNLDKRASEHLHQLMFDLCREFNKTFVVVTHNEALAE
                     RAQRVIRIEDGGIDSDIRKA"
     gene            complement(5071..5907)
                     /locus_tag="PK937_13340"
     CDS             complement(5071..5907)
                     /locus_tag="PK937_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33604.1"
                     /translation="MRFLKFYLILFLTVASAAFSQKDRLVVLTYKFNEGDKYRVAVST
                     YKNFSTISSAYNNSVYGETAYDIHQNILHVDGEGSAQIANDIELTRYTINGQNLTYKM
                     AKLFANDHVEIGVDRFGTLLEKSLTLKNGVLAESGIENIWRYMFIPFPEKTLKIGETW
                     TLNDAAGDSAIILPFKKLLKITDPTLTVRYKLEEADKSTAKIKLSLEVNGEGNIPDHK
                     EQTKINFLIILEGTMVYSFSEGRILNGSLVTQLAGTGTVGSKAVEYSGSLNTTFTIEE
                     AQ"
     gene            complement(5915..7144)
                     /locus_tag="PK937_13345"
     CDS             complement(5915..7144)
                     /locus_tag="PK937_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNH33605.1"
                     /translation="MKKMPSEWFIALRYLKPRKDNFFVTLIGGISVLGVMVGVMSIIL
                     VLTVLNGFETEIKNRFIGFDAHGKIKMQNEIPMTSPDSVENILRKQYPINGAAPYILE
                     KAMITSRQSSHIVFVKGTDEKRLTQVSDLSKNMAAGKIDFEVQSEGLSGILLGLSVAI
                     NLDVSTGDTVTVISPSGVTGPFSAPPMRRYVVTGLFKTDMYEYDNAYVFISIPEAQKL
                     FEKDEAITGLDIRFENIAQASEYENAIRTEIGESFVYETWYDMHRDLYAAMKLEKWGS
                     LLLLSMIIMVAGFNIVSTLIMVVMQKTQEIGILKSIGANQKFISSIFVRQGLIVGSIG
                     IVAGCIVGYSICFIQLQWGIFKLPGDIFFLDAIPMELQWGDFFAVIVISVLLCLFATY
                     YPARKAAKLLPLEALKS"
     gene            complement(7141..8412)
                     /locus_tag="PK937_13350"
     CDS             complement(7141..8412)
                     /locus_tag="PK937_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNH33606.1"
                     /translation="MNLFSYPSFIARRYLWAKRKNGFISIISIISMAGVALGTAALII
                     TLCIISGFENQLITKFINFDAHIRLKTFDGTEPIGDTNMIEERLQGYKSIIGFSPYIE
                     KEAMIRSSQITDGVIVKGINEQRFHSVLDLKKDILKTHVSVSTPLGLRDYISNDSTSK
                     KLPGIVIGKKLADKLAVGIGDKLTLFSLQNSPSFLQQPVVRQYRVTAIYRSGLAEYDN
                     VYVYVDLTEASKLFDYGASVSGYEIKLNDPESAKPTAEALNGELGFPYYAKTWYDNHR
                     NLFGWLETNNFIMVIIFSLIIFVAAFNIVGTLFMIVIEKTKEIGVLRSMGATRSEIRR
                     IFLAEGLIIGFIGAVVGSILALALMLSQIYFKWLSLDSDVYFMDSVPIEIRGEYFFVI
                     ASFSVLLCLLATLYPSRKAAFLDPVEAVRYE"
     gene            complement(8409..9146)
                     /locus_tag="PK937_13355"
     CDS             complement(8409..9146)
                     /locus_tag="PK937_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33607.1"
                     /translation="MEKRLPEVKENDINELIRLLKEEEEWYKKKLKYDDINEKIFSVS
                     ETVKRIIDNTETECKLIEVRLEDARKLTAYLPDLEREHETLTREFHRLTKIREKQDQI
                     TKQGLKDRIELELLQQQIDQLIVTKSDWLIRLDQTQKELTRLREEYLLIENDLKLFPD
                     VEKRLIANEFFIEQAHKAEKDVRILSDEMQKLKHKLTYKTMIRRESGLLDALKTELQS
                     IQYDPQKHFECREALFHLTDRIAQGES"
BASE COUNT         2825 a         1887 c         1649 g         2828 t
ORIGIN      
        1 catgaagtag catgggaagt tgagctcatg ttttttatgc gtgaaaaaat cagaaacacc
       61 acgcatacgg tatccaatag caaaattggc cttttattct tgagaaaagg ctttctcgat
      121 gaagccattt ccgaatttca acatacgatc gagcatgatc cggatcttgt ggaggtttac
      181 aatcatttag gactcgccta tctccatcgc ggcgattttt cgttagctat cgaatctatt
      241 caaaaaggta tcgaactgag acctcaattt cctgatctgc gcaataacct cgggtttgca
      301 tatggtaaag cgaaaaagta tagtgaagcg ataacggaat atgataaagc attagcgatt
      361 aacccccact atgtagtcgt gcatctaaat aaagcgataa ctttcctcga aagtattctc
      421 aacaatggcg atacgggaag tcttccgacc gttcaagatc gaatactcac cgtaagctct
      481 atttttaaaa ccacattgat gacaaatcct gattttgcaa attatcgttt ttatacattt
      541 agatttgaaa agattttaaa acacttgacc gcatcagctt atgtccaagc cattgacatt
      601 attttggaag tcaaagaatc tattcaaaaa ccaagccttg atgagactat tcacggattt
      661 tatttaaagt tccttttcgg aggagccggt aaggatgaaa aagttattaa cgactaccgc
      721 gcacaattag aacaacatat aaacgaaaat cctacgtacg ccgatttgca taattcactt
      781 ggtttagtat atctgattca atgcagaaat ttattcctta aggccatgtc gcaattcaaa
      841 aaagcttacg aaataaaccc atcttataaa accgcatata aaaacttccg cttagtgcag
      901 aatgacgggc gcgagttcct caatctactt cgtgctatct taaagtaatt ccaaccttta
      961 tcatcttttt ctcaatttct tttatttctc attccatgac tgttttatca tttcacagta
     1021 taatctaata atggtagtct cttcctcaaa aaacatcaat atttgtattt ttttgagaaa
     1081 atgaattgcc atcgaacaag tataataatt ttgagtgtta agcccatcac agaaacactg
     1141 taaactgcat cacagaaaac aatttaaact ctataaaaac atacaattga atttttaatg
     1201 ataacattgg tacgtttttt gatctaattg attatcagtt ctaaataatg aaagaaggtt
     1261 atctgatgat ccccagtgtc aagctaaata gagaagtttt cgataacatg aaactttgta
     1321 accgaaaaac ggtgcttgtg gtagaagatg aacccggaat tcgttttgta acgtcagaat
     1381 tcctaaagat gagtaatttt caggtcgtaa ctgcggaaaa cggtcaagag gctatgaaaa
     1441 ttatagtgga taataaaata gatgtcgtgg taacggatat catgatgcct aaagtaaacg
     1501 ggttggaact tttgaaattt gttcgccgac tcaaaggtga gtcattccct gtcgttgttg
     1561 tatccggttc ggatttagaa agtttgcacg tcattgaaag tgaacataca cgcacgctga
     1621 gaaagcctta cggattagat cgtttggcaa aagaaatact aagtatatct caaaactaaa
     1681 ctcattaact acctttaatc aaagttattt catcaatttt atcgtattct tccaaacaaa
     1741 cttttacttt atcacatatt tctgaagtta ttttttttcc ataaatatcc gattggatcg
     1801 gtaactcgcg acatccccta tctataagta cggcgagctg aattcgtgat ggtcgcccga
     1861 aatccataag ttcatccatt gccgcacgca ccgatcgacc tgtaaaaagc acatcatcga
     1921 ctagcaccag cgggcgctcg ttgatgttaa accgtatctc ggtactctgt actaatggtt
     1981 gttttagctt cgctcttgta tcatcacgat aaaagctcac atccaatact cccatgggca
     2041 gttcaatatc ggaaaatttt ttaaccaaaa tttgtaaacg acgcgcaatg aattctccgc
     2101 gggtcctcat ccctattaaa gctaatgact caagacttgg attggaagaa atgagcgatc
     2161 gcgtcatatc ttcaagcatg gtattaattt cctgcgccct tgctatgatt atactttttt
     2221 ctgcttcgtt catttctgtt tccattattt attggtatta cgttgcaaac gatgcatttg
     2281 accaagtgct aattcgaaaa acataagaat tatcgcgacg gcaataaacc atttccaact
     2341 ttcgatacca aatcgagatt gtaaaatata gttttcaata gattcatttt cagttaaaaa
     2401 agtaacattc attgtgccgc taaatatttt aagagagtcc gattcccata tagcaaattc
     2461 attttcttgg cttggaatat tggcgcttac caacgtaagc actcgggaat tgtttttatc
     2521 cacaagagaa taattacccg ccatatcaag tttatcaatt tgagctattt taatgttatt
     2581 ttggactact aaagacggga tgtagtcgct ctgttcgcca gtaagattgc gcatacgaac
     2641 catatcattg gcgtcccttt gaatacgaac tctgacagcg tcaccgacaa cgaattgatt
     2701 tttatcattt tctttaccat acagagcata taaacaacga tgcataagcg gtacgaaaaa
     2761 cgcacgaatt ggaagatcat tccaagtcat aacgggcatt gatgtcatga gcataaaata
     2821 accattgtga atggcatatt cggttaaaaa attaccgctt tttccaaacg ttaggatgtt
     2881 ttgaccatgg cgtgagtctg tattaaagta acgataaaat tttggggact caatattctt
     2941 caaagaagtg cggtccgtaa aaatatctgc aatcaaagga tgcggctgag catcaaattg
     3001 aagttcaata aacgaatggt tttcgctatt gagcgaaacc atctgaatca aacgcgatac
     3061 atctagccta tccgcgattt gattattaaa ttgggtgata tccgttttag tgcccggaat
     3121 catgagaatt ccaccaccct ctttcaagaa ttctacgact cggttagtct gagattcgtc
     3181 ccaacggcca gggtttacaa gaatcagaac atcaaatttt ttcaggtcaa cggttgcaag
     3241 tttgtcaaac aaaactgatt tggtaatgat gttacctgct tgttggtcat gcgctttaag
     3301 cgcaagctct ataaattctt tgtctgcgag ttcaccggcc acaagaactt tgatatctga
     3361 tggaacataa taatgaaaat aatatctatt atttgctaaa aaaccatcat catctaactc
     3421 cgcgtaaccg gaaacaaagc ctgattcgga tgctggaaat gaaatgcgca catcggtatc
     3481 agttttagca tcaacctgta aaacggactg gcctactcgt ttcccattag catagacgtg
     3541 caaaatcctt tcaccggact gggttgacaa tgaatttcgt atcgtacaca ccatttcgat
     3601 cgggtgactt ttttgaaaaa atcgattttc cgtctcagct tttaatatgg ctaggtccga
     3661 ttccggtttt ccatcaatag cgatgacaac taaatgtggc ttgatatggc cgtaatctgg
     3721 aatttgagta ttaaatcggt cggatttttg caaatcagag atgatataaa tttcacggtt
     3781 ggggttttta gcctgtgcta aaaggctcac ggattcgtgc agagctttat ctatagcaaa
     3841 tgaataattt tcgataacaa tatttttaag tcgagcccgt gcttcagaaa aattagaact
     3901 ccaatttaaa ttagaagctt tttttaatcc tgacgcatct ataataacaa attcatcgcc
     3961 ttcttgaaga tgcgaaatga cttccattgc cttatttttt atgcgttcca gcacttgtcc
     4021 gcgttgattt acggacgaag cgctgagtga attatcaagt ataagtacaa cagaagtttt
     4081 agcatgggcg tttatgtttc cgtttgttgc ggtccacttc cacaccggtc gcccaaatgc
     4141 tataacgagg cacgctacga tcatcgctct gataattaac aataataact gttgccaacg
     4201 taacttttta atttgttttt tttggagtgc ttttaaaaaa cgattggagc taaaaggaac
     4261 aacacgtgct ttttttcgat tgagcagatg aatcaatatt ggaatagatg ccaataccag
     4321 accatacaat aaaaaagcat ttaagaaact aaacatatca tgctttccgt atatcggaat
     4381 ctattccgcc gtcttcgata cgaatcactc gttgtgcacg ttcagccaaa gcttcgttat
     4441 gtgttaccac gacgaatgtt ttattaaatt cgcggcaaag atcaaacatg agttgatgca
     4501 ggtgctcgct agcgcgctta tctaaatttc cggaaggttc atcggccaga ataaggcccg
     4561 gatcattcat caaagcgcgt gcaaacgcaa cgcgttgctg ttctccgccg gatagctcag
     4621 agggtctatg ataataacgc tcggcaagac ccacattttt caaaagattt ttgccccttt
     4681 cataacttga cggatcgttc gaaggttttc cggcaatcaa tgcgggcata attacgtttt
     4741 ccagcgccgt aaactccggc agcaaataat ggaactgaaa aacaaaacca acacgcgcat
     4801 tacgcaaacg tgccatagca acgtctgata actgactcac ctgtgtgttt tcaaagaaaa
     4861 catcaccgga agaaggctta tctaatatac ctaaaatgtg caatagtgta ctttttcctg
     4921 cgccggatgg tcctaaaatt acaataaatt cgccctcatg tatatctata ctaatgtttt
     4981 ttaaaacagt tagctcacct ttggccatgg tataattttt actaatggct tgtgcccgaa
     5041 cgagaatttt tttggattcg gccgaagcta tcattgcgcc tcttcgatag taaaagttgt
     5101 attaagcgat cccgaatatt cgaccgcctt cgaaccgacg gtacctgttc cggcaagctg
     5161 agtaaccaaa gacccgttaa gaattcgccc ctccgaaaaa ctgtacacca tcgtgccttc
     5221 gagaataatt aaaaaattaa tttttgtctg ttctttatga tcaggaatat ttccctctcc
     5281 attaacttcc aatgatagtt tgatcttagc cgttgactta tctgcttctt ccagcttata
     5341 ccttacagta agcgtcgggt cagttatttt gagtaatttt ttaaacggta aaatgatcgc
     5401 tgaatcacct gctgcgtcgt ttaatgtcca tgtctctccg attttgagag ttttttcagg
     5461 aaatggtata aacatataac gccaaatgtt ttcaataccg ctttcagcta aaactccgtt
     5521 ttttaaggtc aacgattttt ctaacaatgt cccaaaacga tcaacaccga tttcaacatg
     5581 atcattggca aaaagtttag ccattttata tgtaagattt tgtccattga tcgtgtatcg
     5641 tgttaattcg atgtcattag ctatctgcgc actgccttca ccgtcaacgt gcaagatatt
     5701 ttgatgaata tcataggcgg tttcaccata tactgaatta ttgtatgcgg acgaaatcgt
     5761 tgaaaaattt ttatacgtac tgacagcaac gcggtattta tcgccttcat taaatttgta
     5821 agttaataca acaagcctgt ctttttgtga aaaagctgca ctcgcaacgg ttaaaaacaa
     5881 aattaaatag aattttaaaa atcgcataaa atccttatga ttttaaagct tcgagtggta
     5941 aaagttttgc ggcttttctg gcggggtagt acgtagcgaa aaggcacaat aaaacggata
     6001 tgacgatgac cgcaaaaaaa tcaccccact ggagttccat aggtatggca tccaaaaaga
     6061 aaatatctcc cggcagctta aaaatacccc attgtagttg aataaaacaa atcgaataac
     6121 ctacaataca tccggcgaca attcctatgc tcccgacgat cagaccttgt ctgacaaaga
     6181 tggacgaaat aaatttttga ttagcgccta ttgatttgag aattccaatt tcttgtgttt
     6241 tctgcataac gaccatgatc aatgtgctaa cgatattaaa tccggctacc ataataatca
     6301 tactcagtag taaaagcgac ccccattttt ccaatttcat cgcggcatac aagtcgcggt
     6361 gcatatcata ccacgtttca tatacaaaac tctcacctat ctcggtgcgt atcgcatttt
     6421 cgtattcgga cgcttgtgca atattctcaa aacgaatatc caagccggta atcgcctcgt
     6481 ccttttcaaa tagtttctgg gcttccggta tggatataaa tacgtaagca ttatcatact
     6541 cgtacatatc ggttttaaac aaacccgtta ccacataacg acgcatgggt ggagcgctaa
     6601 aaggccctgt aaccccggaa ggactaataa ctgtcaccgt atcgcctgta ctcacatcaa
     6661 ggttaattgc aacactcaaa ccaagaagta ttcctgataa tccttcggat tgcacttcaa
     6721 aatcaatttt accggcagcc atgtttttag aaagatcaga aacttgagtt aaacgctttt
     6781 cgtcggtacc ctttacaaat acaatgtggc tggattgacg gctggttatc attgcttttt
     6841 caagtatgta aggcgccgct ccgttgattg ggtactgttt tcgcaaaatg ttttctacgg
     6901 aatccggtga tgtcatagga atttcatttt gcatttttat tttaccgtgt gcatcaaatc
     6961 ctataaaacg gtttttaatt tccgtttcaa acccattgag caccgtaagt acaaggatta
     7021 ttgacattac cccaaccatt acccctaata ctgaaatacc gccgataagt gttacaaaaa
     7081 agttatcttt gcggggtttc aggtaacgta aagctataaa ccattccgat ggcatttttt
     7141 tcattcgtat cgcacggcct ccacaggatc taaaaaagcg gccttacgac tcggatacaa
     7201 cgtcgccaac aggcataaca atacggaaaa tgaagcaatc acaaaaaaat attcgcctct
     7261 tatttctatt ggaacagaat ccataaaata gacatcggaa tccaaactta gccatttgaa
     7321 atatatctgt gacagcatca atgcgagagc caatatcgaa ccgacaaccg cgccgataaa
     7381 accaataatg agcccttcgg caagaaaaat gcgacgtatt tcagaacgcg tagcacccat
     7441 agatcgtaaa acgccaatct ccttagtctt ctctatgaca atcatgaata aggttccaac
     7501 aatattaaac gccgcaacaa aaatgataag actaaaaatt atcaccatta tgaaattgtt
     7561 agtttctaac caaccaaata agttacggtg attatcatac caggttttcg cgtaatacgg
     7621 gaagcctaat tcaccattaa gagcttcggc cgtgggtttc gccgattccg gatcatttag
     7681 ttttatttca taacccgaca cggacgcacc gtaatcaaac aatttagatg cttccgtaag
     7741 atccacgtaa acatagacat tatcatattc cgctaacccg cttcggtaaa tggccgttac
     7801 acgatactga cggactactg gttgctgcaa aaacgacggt gaattttgga ggctaaacaa
     7861 agtaagttta tcgcctattc cgactgcaag tttatcggct aatttttttc caataacgat
     7921 acccggtaat ttcttggagg tactgtcgtt tgaaatataa tcacgcaagc ccagtggagt
     7981 tgatacggat acatgtgttt tgagaatatc tttttttaga tccaagaccg aatggaaacg
     8041 ttgttcgtta attcccttga caataacacc gtctgtaatt tgagacgaac gaatcatggc
     8101 ctctttttca atataagggg aaaatccgat gatggattta tatccctgaa gtcgttcctc
     8161 tatcatattc gtatcaccta tcggttctgt gccatcaaat gtcttcaaac gaatatgtgc
     8221 gtcaaaatta ataaatttag taatcaattg attttcaaaa ccggatatga tgcacagggt
     8281 tatgataagc gcggctgtgc ctaaggccac gccggccatg gaaataatgg aaataatgga
     8341 aatgaaacca tttttacgtt tggcccacaa ataacggcga gctataaaag aaggatacga
     8401 aaacaaattc atgattcccc ttgagcaata cgatcggtga gatgaaacaa tgcctcacga
     8461 cactcaaagt gcttttgcgg gtcatattgt atgctttgaa gttctgtttt gagcgcatcc
     8521 agtagtccgg attcacgtcg aatcattgtt ttataagtca atttatgttt tagtttttgc
     8581 atttcgtctg acaaaatcct cacgtctttt tcggctttat gtgcctgttc tataaaaaat
     8641 tcattcgcaa ttaaacgttt ttctacatcg ggaaataatt ttaaatcatt ttctatcaaa
     8701 agatattctt cccttagacg cgttaattcc ttttgggttt gatccaatcg tataagccaa
     8761 tcagacttcg taacgatcaa ttggtctatt tgttgttgta acaattcgag ttcgatgcga
     8821 tccttaagac cttgtttggt aatctgatcc tgcttttcac gaattttagt caaccgatgg
     8881 aattcacgcg tcagagtttc atgctctcgc tccagatccg gaagataagc ggttaacttg
     8941 cgcgcgtctt ccaaacgaac ttcaattaat ttacattccg tctccgtatt atcaatgatg
     9001 cgtttaacgg tttcagaaac agaaaagatt ttttcattaa tatcatcgta tttcagtttc
     9061 tttttatacc actcttcttc ttcttttagc aaacgaatta attcgttaat atcgttttct
     9121 tttacttcgg gtaacctttt ttccataata tagtttacca attttacaga ttaaaacaaa
     9181 gtttttgat
//
LOCUS       DAOQGF010000122         9159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3561_length_9159_cov_6.903339, whole genome shotgun sequence.
ACCESSION   DAOQGF010000122 DAOQGF010000000
VERSION     DAOQGF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3561_length_9159_cov_6.903339"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>9159
                     /locus_tag="PK937_13360"
     CDS             <1..>9159
                     /locus_tag="PK937_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH33608.1"
                     /translation="DGDMDIAAANSGSNSVSIRLNDGAGNFSGSTTVYVGSGPSSVYA
                     ADFDGDGDMDIATADLSDYVSIRLNDGAGNFSGTTDVAVGWSPLSVYAADFDGDGDMD
                     LATANSVSNDVSILINAPGYFNVTSVTPLQNQGNVGISSNIFVNFPQDINVATLNNAT
                     IKIRGSHSGLHTANYFYEASLSIDPDTDFKFGEVVTVTLTDGIIATNTDTLNPYSWQF
                     TVGAFPATGTFTTLPGATVGSTPFGVSLADLDGDGDLDMAAANDVDGTVSIRFNDGTG
                     NFSGSTDLSSGTNSRAVTAADIDNDGDLDLLIASSGFVASYINNGLGSFSGPNNFAIG
                     GNGQSVFAADFDGDGDVDFVTANSGSNNVSIRLNDGAGNFSGTTDVAVGNLAVGVTGG
                     DVDSDGDIDLLASNYNDNTVSILLNDGAAGFSSGGTSSVSFGPVMVRLADLDADTDLD
                     LVTANEGGSDISVRLNDGTGVFSGGSDISGLVSPRSVFLGDLDGDGDVDIAAGNTGGA
                     TTVSIRINDGSANFSGSYNAFVGPGPAWSIDGGDVDNDGDIDIIRVDGSVSGNTTVLK
                     NNNYIVPTSVIPIPNTVGINPTANIVVEYPEDMSSFDAKVFGSLSGYMNTSLFYDNVL
                     YTLTIDPTANFKPGEVVTVIMSGASNVAVSGNILRNIQFQFTAAPSFAAASYSLPASN
                     LAAGTNPGGVATADFNLDGTIDIVATNVSPSEVTIILNDGIGGILSSYTVPVGTNPYG
                     PHVADLDNDGDMDIVTADAGGNTVTTLLNDGAGNFSGPSVAVGSGPYAVQSADIDGDG
                     DLDLVVANANSNNVSVLINDGAANFTGGATVSVQTTPYDIVAGDFDSDGDMDFATANV
                     GANSISVRLNDGAGNFPIASDPAVGTNPQGIQAADLDGDGDIDILTANVGSNDASVML
                     NDGSGNFFGGQTPTVGAFPYSIIASDLDGDGDVDFATADWNANTVSVRYNDGAANFSG
                     STAISVGSQPYDIAAADLDGDGSIDLITPNAGSNDVSIAFNDAILPSPSGLYAVAQNG
                     QVELNWSAAPGATLYRIYHDVNPNPMVQTDSSVGTNKTIAGLVNGTLYFFRIKAINAT
                     DESGYSNEDAAVPVAGAGQALSLEGIDDEAAIFTSYQNLGVDPSSVTISAWVKPNGTS
                     PSISTIYEGQGIILDAGGYLGLTRGDYTGQDRIWAYLWDGSQIFVGIPYTNGVWTHVA
                     VVLRNGQLYAYKNGVVVDSAVSGNVQNITSGSIGVGVNPYNSLHFEGQIDEMRIWNRG
                     LMDAEMASVQASPSRGDEAGLLANWHFDEGSGSTTFDASPNNRNLTLFNGPTYVVSGA
                     MAPPLSIVSTTPSANGLSIPANSNIIVNFTSDILTDSLNSRNILIRGAQSGDHTFTYG
                     YGAQTANIDPNTDFIPGELVTVTLTRGILAISSDTLKPYSWQFYVQTNLATGFFSAGD
                     TVVVGTFPKAIAAADFNNDGSLDMAVVNQSTNDVSIAINDGSGGFTVLSNVAVGSLPY
                     EIASADFDGDGDLDIAVSNNSSSDISIRSNDGSGNFGVLANISTGVAQLTVAAGDFDG
                     DGDVDLISSESGTQLRVRLNDGTGSAFSPGNVINVQNNPYFITVADFDNDGDLDLTAS
                     NYSSGTFSVRLNDGTGLFGAGGDFTTNGIQPRAIRAADFNGDGLMDIAFCNENSGNVG
                     IRLNDGVGGFSGNEIGVGSLPQSITLGDFEGDGDMDVAAVNYGSTNVQTIVNDGAGNF
                     VAGSSYGTGNGGVILSSGNFSGTATMEIAAADNTANVIYVLKNAVILPTTTLVTSKGD
                     GGSGSLRDAIDYANSHAGPDTIIFNVSVDSVLLASQLPDITDDSLFINGDYNTDNIPD
                     VVIDGINNGINEAFVRVAANDITIKGLTIIRFVQGSEAALTISNAARRAKILYNYIGT
                     DGTALYSNVNNIDILAPAGGGQHEIRGNVISARGYGIFFYGSSNDLDSTKIVDNKIGT
                     NAAGSAPLTYAPSHLSGIYIQNGRGLKIHSNIISGWSGAGALAINFGYTGFSYYAKEV
                     EIFDNKIGTDFSGTAAIPNERGIYFDAGSNANFIGKPGLGNIISGNTGNGIEIWSDSN
                     VIEDNFIGVDISGNAPLANVGMGIQLQNASVNTIGNGNTISGNANHGIFIFSNGGLAD
                     ENTIINNNIGVGADGVTPVGNNQMGVRIYSLLGARRNLLLDNAIAFNNFHGIEMDGSA
                     SADSMIAHGNRIYSNNLGGMNYVAGSNGSITPPMISFMSMDSVVHGTSDPNAFVQIYV
                     DSTNQGRLRIGTGVADGAGNFAVPVTLLGGEAPFLTALQSSTGRTSGFSVPISLTLSD
                     LSVVNSLADDGSPGTLRAAIDYANSHVGPDTIRFDISLLGQTINMNSNLPDITDDYMV
                     INGDVDGDNIGDVQLFGLDPTADKGLNILSSYNEIRNLRITDFYNNSSGIDIDGGNYN
                     IITGCYVDDNEVGIHIKNGGRGNRIGDGTIQGRNYIFNNTYNGSPGIIIEHVGSDSNI
                     VAGNFIGTEDGVTPSGNGTDGVIVRLGAKYNRIGDANAQIYNVISANTNHGILIESDS
                     NMVVRNLIGTDSTGTVAMGNQGYGIHILNAVGNMIGDGTLAGRNIISNNSSNVLNGAG
                     IMVENSSGTSILGNFVGTDITGLAAMPNWSGGGAGSGVTLDNSSYNVVGDGTDAGRNV
                     FGNNGSGADFPVQIYIQNNSNYNLVAGNYVGTDSTGTSSLGTGNGIGVYGSVNNQIGL
                     NAGAAANVIAGNGGDGIILNNADSTKVFQNYIGVDRTGATALPNNNGIVLENGTQNAL
                     IGNNTGSGGNIVSGNSNQGIRIQDVGTDSNLIIGNLIGTDLSGSTAIPNGNDGIQIDG
                     GSANQVGEIGSGNVISGNSGNGIAIIGNTGISAYNVVLHNLIGVAIDSVTALGNAGNG
                     VFIAGISTFAHNNEITRNRIAYNTNFGITADQTGFQVDSDHFFANEIYSNGGGGISLV
                     AGAHQNVWAPVITSVDFDSTVSGYSGKNALIQIYADSADQGRIYLDSVYADASGNWSI
                     KVPPIPGMYYTAIQDSFFHSSAFSAPVLAIAVDPLLVINTNDAGAGSLRD"
BASE COUNT         2251 a         2131 c         2377 g         2400 t
ORIGIN      
        1 gcgacgggga catggacatc gctgcggcca atagtggttc aaatagtgtt tcgatacgcc
       61 tgaatgacgg cgccggtaat ttttccggaa gtaccactgt atatgttggg agtggtccat
      121 cttcagtcta tgcggcggat tttgacgggg atggggatat ggacatcgcg acggcagatt
      181 tgagtgatta tgtttcgata cgcctcaatg acggcgcagg caatttctca ggaactaccg
      241 atgtcgcggt ggggtggagt cccctttccg tctatgcggc ggactttgac ggggatgggg
      301 atatggatct tgcaaccgcg aacagcgttt caaatgatgt ttcgatccta ataaatgctc
      361 ccggttattt caatgttaca tccgtaactc cgctgcaaaa tcagggtaat gtaggcattt
      421 catcgaatat cttcgtcaat tttcctcaag atatcaatgt ggccacttta aataatgcca
      481 cgatcaagat ccgcggttcc cacagcgggt tacatacggc taattacttt tacgaagcca
      541 gcttatccat cgatcccgat accgatttca aatttggtga agtagtaacg gtcacactca
      601 ccgacggtat catcgctacc aacacggata cgctcaaccc ctattcctgg caatttaccg
      661 ttggtgcttt ccctgcgacg ggaactttca ccacgctgcc cggagcaacg gtcggctcca
      721 ctccgttcgg agtcagtctg gccgatctgg acggggacgg tgatcttgat atggctgcgg
      781 ccaatgatgt cgatggaact gtctcgattc ggttcaatga tggaacaggc aatttttccg
      841 gcagtactga tctttcatcc ggaacaaatt caagagccgt gacagccgcc gatattgaca
      901 atgacggtga tttggaccta ttaatagctt cctccggttt tgtagcgtcc tatatcaata
      961 atggtttagg tagtttttca ggcccaaata attttgcgat cggaggaaac ggccaatctg
     1021 tttttgcagc ggactttgac ggcgacggcg atgttgattt tgtcacggcc aacagcggat
     1081 cgaataacgt gtcgatccgt ctcaacgacg gcgcaggtaa tttttccgga actaccgatg
     1141 tggcggtggg aaatcttgcg gtcggcgtaa cgggaggaga tgttgacagc gatggggaca
     1201 ttgacctgct tgcgtcgaat tataacgaca acacagtatc cattttgctc aacgacggcg
     1261 cggcgggttt ttcttccgga gggacttctt ctgtaagttt tggtccggtc atggtcagac
     1321 tggctgatct ggacgccgat accgatctag atctggtcac ggccaacgag ggcggaagcg
     1381 atatatccgt ccgcctcaac gacggcacag gtgttttttc cggtggttcc gacattagcg
     1441 gtctggtttc tccacgctca gtatttctcg gtgatcttga cggcgacggc gatgtggaca
     1501 tcgcggccgg aaatacaggc ggggcaacta cagtttcgat tcgcattaat gacgggtccg
     1561 ccaacttttc gggttcttat aacgcgttcg tcgggccagg gcctgcatgg tcaatcgatg
     1621 gaggagacgt agacaatgac ggcgacatcg atataatccg cgtcgacggt tcggtcagcg
     1681 gaaatacgac agtcttaaaa aataacaatt atatcgttcc tacttctgtc attccgatcc
     1741 caaacaccgt tggaatcaat cctacggcca acattgtggt ggaatatcct gaagatatga
     1801 gttcattcga cgccaaagtt tttggttcgc tgagcggcta tatgaataca tcgctgtttt
     1861 atgataatgt cttgtatact ttgacgattg atccgacggc caattttaaa ccgggtgaag
     1921 tggttactgt gatcatgtct ggcgcttcaa atgtcgccgt cagcggaaat attttgagga
     1981 atattcaatt tcaatttacg gccgcgccgt cctttgccgc ggcgtcctat tctttaccgg
     2041 catcaaatct tgccgccggg acaaaccctg gcggcgttgc gacggcagat tttaacctgg
     2101 atggtacgat cgatatcgtg gccactaatg tttcaccgag tgaagtcacg atcattctta
     2161 acgacggcat cggcggaatt ttatcaagtt atacggttcc ggttggaacc aatccgtacg
     2221 gtcctcatgt ggccgatctc gacaatgacg gcgatatgga tattgttacc gccgacgccg
     2281 gcggtaatac tgttacgaca ctgcttaacg acggagccgg taatttttca ggaccaagtg
     2341 ttgcagtagg ctccggtccg tatgctgttc aatccgctga tattgacggc gacggggacc
     2401 ttgatctggt tgtggcgaac gcgaattcaa ataatgtgag cgttttgatc aacgacggcg
     2461 cggcaaattt taccggcggc gctacggttt ctgtccaaac aacgccgtat gatattgtcg
     2521 ccggagattt tgacagcgac ggcgatatgg attttgcgac ggccaatgtg ggggcaaaca
     2581 gcatatcggt tcgtctaaat gacggcgcgg gtaatttccc gattgcatcc gatccggctg
     2641 ttggaaccaa tccgcagggc attcaggcgg cagatttgga tggcgatgga gatatcgata
     2701 ttctgacggc aaacgtagga tccaatgatg catcggtaat gttaaatgac gggtcaggaa
     2761 actttttcgg tggtcaaacc ccaacggtcg gtgcttttcc ttactccata attgccagtg
     2821 atcttgacgg cgatggcgat gtggattttg ctacggcaga ttggaatgcc aatacggttt
     2881 ctgtacgtta taacgacggc gcggccaatt tttcaggaag cacagcgatc tccgtcggat
     2941 cacagcctta tgatattgcc gcagcggatc ttgacggaga cggttctatc gatctgatta
     3001 cgcccaatgc gggctccaat gatgtctcga ttgcattcaa tgatgcgatt ctaccgtcac
     3061 caagcggttt atatgcggta gctcaaaatg ggcaggttga attgaactgg agcgctgcac
     3121 ctggcgcaac attataccgc atttatcatg acgtcaatcc caatcctatg gtacagacgg
     3181 attcttcagt tggaacaaac aagacgatcg caggacttgt taacgggact ttatattttt
     3241 tccgtatcaa agcaatcaat gcgaccgatg aaagcggcta ctctaatgaa gacgcggcgg
     3301 ttccggtagc aggtgccggt caggcgctga gtcttgaggg tattgatgat gaggcggcga
     3361 tctttacgtc ataccagaac ttgggtgttg acccgtcatc ggtcacgatc agcgcctggg
     3421 ttaagccaaa cggtacgtct ccgtcgattt ccacgatcta tgaaggccaa gggattatcc
     3481 ttgatgccgg aggttatctc ggattaacgc gcggcgatta taccggtcag gaccgtattt
     3541 gggcctatct atgggacggg agccaaatat ttgtaggaat tccatatacg aacggcgtct
     3601 ggactcatgt ggcggtcgtt cttcgcaatg gtcagcttta cgcctataag aatggtgttg
     3661 ttgtggattc tgcagtcagt ggaaatgtcc agaatattac atccggctcc atcggtgtcg
     3721 gtgtgaatcc atacaatagc ctgcattttg aaggtcagat cgatgaaatg cggatctgga
     3781 accgcgggtt gatggatgcg gaaatggcat cggttcaggc tagcccatca cgtggggacg
     3841 aagcaggtct tttggcaaac tggcattttg atgaaggctc aggttcaacc acatttgacg
     3901 cttcgccaaa taacagaaat cttacgctgt tcaacggccc tacatacgtt gtatctggcg
     3961 cgatggcgcc tccgctgtca atcgtctcga ccacaccatc ggccaatggg ctttctattc
     4021 cagcaaacag caatattatt gtaaatttca catcggatat tctaacggat agtctgaatt
     4081 cgagaaatat tctcattcgt ggtgcgcaaa gcggtgatca tacttttaca tacggatacg
     4141 gtgcgcaaac ggcaaatatt gaccccaata cggattttat tcccggtgaa cttgttaccg
     4201 tcactttgac ccggggtata ttagccataa gcagcgatac gcttaaacca tattcatggc
     4261 agttctatgt tcagacgaat cttgcgacgg gtttcttttc cgcgggcgat accgttgtcg
     4321 tgggaacttt tccaaaagcc attgcggctg ccgacttcaa taacgacgga agtctggata
     4381 tggcggttgt caatcaatcg acaaacgatg tttccattgc catcaatgac ggctcgggcg
     4441 gcttcaccgt tttgtcaaac gtagcggtgg gcagtcttcc ttatgagatc gcgtctgctg
     4501 attttgacgg cgatggcgat ctcgatattg cggtttctaa taattccagc tcggatatat
     4561 ctattcgttc aaacgatgga agcggcaatt ttggtgtgct tgccaatatc agcacgggtg
     4621 tagctcagtt gaccgttgcg gccggtgact tcgatggcga cggggatgtc gatctgattt
     4681 cttcggaaag cggaacccag ttgcgcgttc gcttgaacga cggtacagga tcggccttct
     4741 cgcccggcaa tgtgatcaat gttcagaata atccttactt tattacagtc gccgatttcg
     4801 acaatgacgg cgatctcgat ctgacggcat cgaactacag cagcggtaca ttctccgtac
     4861 gcttgaacga tggaacgggt ctttttggcg ccggcggaga ttttacgacc aacgggatcc
     4921 agccgagagc catccgtgcg gccgatttca acggcgacgg gcttatggat attgcttttt
     4981 gtaatgagaa cagcggtaac gtagggatca gattgaatga cggcgtcggt ggattctccg
     5041 gcaacgaaat tggcgtcggt tcattaccgc aatccataac tttgggtgat tttgaaggag
     5101 atggcgacat ggacgtggct gccgtgaact acggatccac gaatgtccag acgatcgtca
     5161 atgatggcgc cggtaacttc gtagcaggtt cttcctacgg cacgggcaac ggcggtgtta
     5221 tcctttcatc aggaaatttt tcaggcacag cgaccatgga aattgccgcc gccgacaata
     5281 ctgcgaatgt aatctatgtt ctcaaaaacg cggtcatttt gccgacaaca actttggtta
     5341 catcaaaagg ggatggcggt agcggatcac tgcgcgacgc aatcgactat gccaactctc
     5401 acgccggtcc tgatacgatc attttcaatg tatcagtcga ttccgtctta ctcgcctctc
     5461 agttacccga cattaccgat gattctctat ttatcaatgg agactataat accgacaaca
     5521 ttcccgacgt tgtaatcgac gggatcaaca acggtatcaa cgaagcattc gtgcgcgttg
     5581 ccgcaaacga tattaccatc aagggtttga cgatcattcg gtttgtgcag ggttccgaag
     5641 cggcactgac gatttcgaat gcggcacgcc gggcgaaaat actctataat tatatcggta
     5701 ccgacggaac ggcactctac agcaacgtga acaacatcga catcctggcg ccagctggcg
     5761 gcgggcaaca cgaaatacga ggcaacgtca tttcagcgcg gggttatgga atattttttt
     5821 acggcagttc caatgatctt gattcgacca agattgtcga caacaagatc ggaacgaatg
     5881 cggctggatc ggcgccccta acctatgccc caagtcatct ttcgggtatc tacattcaaa
     5941 acggacgtgg tctgaagatt catagtaata ttatttccgg atggagcggc gcgggcgccc
     6001 tggccatcaa ttttggctac accggttttt cctattatgc aaaagaagta gaaattttcg
     6061 ataataagat cggtaccgat ttcagcggca cagcggcgat tcccaacgaa cgcggaattt
     6121 actttgatgc gggttctaac gcgaatttta tcggcaagcc cggtctcgga aacattatct
     6181 ccggcaatac aggcaacggc atcgaaatat ggtcagattc caacgtgatt gaagacaact
     6241 tcattggcgt ggacatctca ggtaacgcgc ctctcgcaaa cgttgggatg ggtatccaat
     6301 tgcagaatgc cagcgttaat acgatcggta acggcaacac gatttccggg aacgcaaatc
     6361 atggtatctt catattctcg aatggcggcc ttgcggacga aaacacgatc attaataata
     6421 atatcggcgt gggtgctgat ggcgttaccc ccgttggcaa taaccagatg ggcgttcgga
     6481 tctattcgct gctcggcgcc aggcggaatc tgttgttgga caatgccatt gccttcaata
     6541 atttccacgg aatcgaaatg gacggcagtg cgagcgcgga cagtatgatt gcccacggaa
     6601 accgtatcta cagcaacaac ctcggcggca tgaattacgt tgccggctcc aatggcagta
     6661 taacccctcc gatgatttct ttcatgagta tggattccgt ggttcacgga acgtcggacc
     6721 ccaacgcgtt cgttcaaatc tacgtggatt ccacgaatca ggggcggctt cgcatcggaa
     6781 ccggcgttgc tgatggcgcg ggcaattttg ccgtacctgt gaccctgctt ggcggtgagg
     6841 cgccatttct gacagcattg cagagcagta ccggtcggac ttccgggttt tctgtgccga
     6901 taagtctgac actttctgat ctgagcgttg ttaattcatt agcagacgat ggatcgccag
     6961 gaacacttcg tgcagcaatt gattatgcca acagccacgt cggtcctgac acaattcggt
     7021 ttgacatttc gcttctcggt cagacgatca acatgaacag caatctcccg gatatcaccg
     7081 atgattacat ggtcatcaat ggagatgtgg atggcgataa tattggggat gttcagttgt
     7141 ttggtcttga cccgactgca gataaaggtt tgaatatttt atcatcctac aatgagatcc
     7201 gaaacttacg aataacagat ttctacaata attccagcgg cattgacatt gacggcggta
     7261 attacaatat cattacagga tgttacgttg atgataatga agtgggtatt catatcaaga
     7321 atggcggccg cggcaatcga attggcgacg gtacaatcca gggcagaaat tacattttca
     7381 ataatactta taatggatct ccaggcatca ttatcgaaca tgttggttcg gattccaata
     7441 tcgttgccgg taatttcatc ggaacagagg acggtgttac accaagtgga aatgggaccg
     7501 acggcgttat tgtccgtctt ggcgccaaat ataaccgcat cggtgatgcg aacgcgcaaa
     7561 tttataacgt gatttccgct aataccaacc acggcatcct gatcgaatcc gacagcaata
     7621 tggtggtaag aaatctgatc ggaaccgatt ctaccggcac cgtggccatg ggcaatcagg
     7681 gttatggtat ccatatcctc aacgccgttg gaaatatgat aggtgacgga actctggccg
     7741 gtagaaatat tatttcaaat aattcatcta acgtacttaa cggcgctggc atcatggttg
     7801 aaaattcttc cggtacctcg attttgggaa attttgtggg aacagatatt accggtttag
     7861 ccgccatgcc caattggtca ggaggaggag caggttctgg tgttacactg gataacagct
     7921 cctataacgt cgtaggagac ggaacggacg cggggcgtaa tgtttttggg aataacggtt
     7981 cgggtgcaga cttccctgtg caaatctata ttcagaataa ttcaaattac aatcttgtag
     8041 cgggtaatta tgtaggcaca gattcaaccg gaacgagctc acttggaacc ggtaatggta
     8101 tcggtgtcta cggcagtgtg aacaaccaga tcggtcttaa tgccggcgcg gcggccaacg
     8161 tgattgcagg caacggcggt gatggtatta tacttaacaa tgcagattca acaaaagtat
     8221 ttcagaatta tatcggcgtt gaccgtaccg gcgctacggc gcttccaaat aataacggta
     8281 tcgtgcttga aaacggaacg caaaacgccc tcatcgggaa caacaccggg tctggcggga
     8341 atatcgtgtc tggaaattct aatcagggaa ttcgtattca ggatgtcgga accgacagca
     8401 atttgattat tggaaatttg atcggcacgg atctttccgg tagcacggct atacccaacg
     8461 gcaacgacgg gattcaaatt gacggcggtt cagcgaatca agttggagaa attggttccg
     8521 gaaatgtaat ttccggaaat agcggtaacg gtattgcgat tattggcaat accggcatat
     8581 ctgcgtacaa tgtcgtcttg cataacctga ttggcgttgc cattgacagc gtcactgcgc
     8641 tgggtaatgc gggcaacgga gtctttattg caggcatttc gacctttgcg cacaacaacg
     8701 aaataacccg aaatcggata gcctataata cgaattttgg aattaccgcc gatcaaaccg
     8761 gatttcaggt ggatagcgat cacttcttcg ccaacgagat ttattccaat ggcggcggtg
     8821 gtatttcact cgttgcgggt gcacatcaga atgtatgggc gcctgtcatc acgtccgttg
     8881 attttgattc tacagtcagc ggttattcag gtaaaaacgc tcttattcaa atttatgctg
     8941 attcggcaga tcaaggccgt atttatctcg actcagttta tgccgatgcg tcgggtaatt
     9001 ggtctataaa agtaccgccc ataccgggta tgtattatac cgccatacag gacagtttct
     9061 ttcattcatc ggctttttcg gcgcccgtgt tagccattgc ggttgatccg ctattagtca
     9121 tcaataccaa cgatgccggt gccggttcac tgcgtgatg
//
LOCUS       DAOQGF010000123         9098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3590_length_9098_cov_6.936194, whole genome shotgun sequence.
ACCESSION   DAOQGF010000123 DAOQGF010000000
VERSION     DAOQGF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_9098_cov_6.936194"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1381)
                     /locus_tag="PK937_13365"
     CDS             complement(224..1381)
                     /locus_tag="PK937_13365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH33609.1"
                     /translation="MNNPPRIAVFKTKFLPYSETFIHDELHAHQRYKPFVLAKKHLNA
                     AQFTDHEVINFLPSDNLLNRIEHYLFNRWRWSWTFKQVLIRNRVCLIHAHFAYSGIFM
                     VPLAKKLNIPLIVTLHGNDVAILLGDQLHQKKWALYKKKYKELLHQASLFLADSQELK
                     DRIVEIGCPAEKVIVHTLGIDLKKFPYQAQKPSLPPRILMVGRFVEKKGFIYGIRAFA
                     AARKDIKNAELHIIGDGELRTEYEAEARSLGVASTVFFHGVRPHEEIVQHMHQAALFL
                     APSVIAANKDRDSGLIVAKEAAACGLPVIGSIHGGIPDIIADGETGFLIPERDVIKMS
                     QCIVEILNNPELWSRMSIASRKKIEEEYNIEKCNERSSSIYDELLKSMQAM"
     gene            complement(1689..2876)
                     /locus_tag="PK937_13370"
     CDS             complement(1689..2876)
                     /locus_tag="PK937_13370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH33610.1"
                     /translation="MSFQNDDHTLFSIAFIAGVPIPSNDVVSQQFINNVAAIMSVNPS
                     WRVKVFAEKPTVGFENVNVQEHYGIAQDIPIEYITVPTLPKKWRKHYSACKAILAAGR
                     EKFDFYFTRHPETLLWSLLAGHKTFFDTYRINLNQRAKYLLWRKYCYTRPHFCGVIAH
                     SKLCAESFLNVCLASSKITIAYNGSHPGRQMDGNETMVLRRQLNIDDGKLVICYAGRI
                     SDSSKGSGMLLDLAQQLPNIIFLFVGGGKKERDESDFDNKIRTLRLSNVRRTGWVEPS
                     AVSRYLQLADILLIPPTAAGLEKKGNTVLPIKTFQYLQAGRAIVAPDLPDCREILQNN
                     QNAILVRPDDLDAARKAVQVLSENSDLRISLSRNAYRDGLKYTWTERAKFISAFIRTR
                     SAS"
     gene            complement(3001..3831)
                     /locus_tag="PK937_13375"
     CDS             complement(3001..3831)
                     /locus_tag="PK937_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNH33611.1"
                     /translation="MKAFILAAGQGKRLLPLTAETPKTLVDVKGTTILEYQLVTLASC
                     GVKDVVIIGGFEIEKLKNYAERYIAIHGLDIRLKMVNNKDYATTNNLFSLWLAKDEMT
                     TEFVVINGDNVFERKALAKVIAQKDMNVSIAIHKNPAYDNEDMKVRINGDRVIEISKE
                     IDNYAANGESIGLRAFRNSGIEAFKQALDSAMIKDVTRNAFFVRAIQHMIDMGENVGW
                     VDVTDFKYGELDFFEDLHHLEIEMSGLMKQTIMLHTNPAGLYARTNGLYQPLMMKNVG
                     "
     gene            complement(4447..5670)
                     /locus_tag="PK937_13380"
     CDS             complement(4447..5670)
                     /locus_tag="PK937_13380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH33612.1"
                     /translation="MRILIVAMKFFPASGGSATYAHHLAQGLHHQGHQVRLLAPKYKR
                     KNISESSDYEIVRMPLTNEVWYGIRIIFATIAIITHYLKFRPDVIWSSTFAGCRSTAV
                     LAFLPGRWIGTIHGGGILRSDEPRNFFVKLSSWLGGLFIRRADAIVTISNEAEKLIRN
                     SVSDKILQSKLKVIYNGIEFNPAVVKTKEDALTFCPQWRNRKIVLTVGRLVEAKGHDL
                     VIQSIHRLRSEIPEILYIIVGEGNTRKNLEMLVKKLGLENYVFFAGYVESSLLENYYA
                     VADIFTMPGRVTREFVEGFGLVFVEAGMRGKAVIGTRVGGITEAIRDNYSGLLIAPDD
                     VENLSEAMKKLLICPATAAVLGRQGREWTINNFSLDVMARHNDRLLLEIYWRKKGRNS
                     LPTNLISTNAMEFSK"
     gene            complement(5921..7849)
                     /locus_tag="PK937_13385"
     CDS             complement(5921..7849)
                     /locus_tag="PK937_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNH33613.1"
                     /translation="MNERDVVATLRFLSFFWLVFLCFFTLARAAFIYEFDEGQMNSYA
                     DIGQLFWIGGKNDIINIAYLTGIPTLLLLVRMFWGVSLTRTERFFRICVYVTVCYISA
                     VVIADWGYYGYFGSRLNASLFNLMSSSDVVFSMLYSQYALLVVIWILLSYLFVRLMQF
                     GFNRYSVVIASKPRLNRASILIALMIVGLWFQASRGFAWHRKFREGSATAFFSNNVFL
                     NTAAINPVNGFTKDAWRMAFRGSKKINWMSEQEAFTVLHRYYPESGSSSTRLLAHEIR
                     GIKPASDQNIVIVLMESMASKFVGALGNKENLTPHLDSIAAQGLLFNNFYSSGIHTYN
                     GVHSTLFGLPAIFKQHPMNTTQALQEHAGLSHMLKERGYRTVFFSPHQATFDNIGGFL
                     GMNAFDRVYDPSDYRDAETANLWGVDDRSLFQSAGKVLDTLADHSPFFSVILTVSNHG
                     PYAIPDDFSPYPGEKDRSHAVRFADQSIGSFMASVKKSKWFDRTIFVFLGDHGARGVN
                     TYDMDLEYLHIPFIIWSKGLPPSIISDLGGQIDIPATIMGLIGGDYTNNTFGIDLLRE
                     RREFLPINSDTKVGCLSKSFLLSLTPKNKPVVSRLSMDIAVPDSQAVVARMSEYLKSI
                     MQSAQWLIDHRQLSLSKK"
     gene            8197..>9098
                     /locus_tag="PK937_13390"
     CDS             8197..>9098
                     /locus_tag="PK937_13390"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HNH33614.1"
                     /translation="MINFFRSIFSSKPKPPVAAIKPHHVTHIGKSLTDDYYWLRDKEN
                     PEVIAYLRAENTYTDALMKPTEALQDTLYREMLSRIREDDRSVPEQIGEFLYYYRTEK
                     DKQYKIYCRKKNGEENELVLLDLNTLARHTPYLALGAYRVSPDHHWLAFTIDHDGSEQ
                     FTLYIKNLETGELSPEHIPNTYYGLEWANNSATIFYTTLDPSLRPHRVYRHSIGAELT
                     SDQLVYEESDERYYLDLTKSRSGRFLLITLASKTTTEVHFLDADHPDKPFQVFQERVQ
                     GHEYNVEHWEDRFFITTNDQAVNFR"
BASE COUNT         2800 a         1997 c         1631 g         2670 t
ORIGIN      
        1 ccgaaagaat tttttctttg gttgtaatgg attcttttat gcgcgacgat atatcttgtc
       61 tcatagtgta gtctcgattc cgtataagca atatggcaac tatttaacat tgtatcaaga
      121 atagtcgttt gatcagtttt cttatcctat acgatttgga ttttttttct ttaatttcaa
      181 ttaccaattt caattgactg atgttgtata agcgttgcaa taattacatg gcctgcatag
      241 attttaataa ttcatcataa atagatgatg accgctcatt gcatttctca atattatatt
      301 cttcttcaat ttttttccta gacgctatac tcatccgact ccataattca gggttattca
      361 aaatttctac tatacattgg ctcattttta ttacatcacg ttcaggaatt agaaaacctg
      421 tttcaccgtc tgcaataata tctggtatcc caccatggat agagcctata accggtaacc
      481 cacaagctgc tgcttctttt gctacaatca atccagaatc cctatctttg tttgccgcaa
      541 tgacgctagg agctaaaaac aatgcagcct gatgcatatg ctgcactatt tcttcgtgcg
      601 gcctgacacc atgaaaaaaa acagtcgatg caactcccaa agaccgtgct tccgcttcat
      661 attctgtgcg aagctcgcca tccccgataa tatgcaactc cgcattcttt atatcctttc
      721 gtgctgctgc aaaagctcga ataccgtata taaatccctt tttttccacg aaacgtccaa
      781 ccatcagtat ccttggcggc aaagatggtt tttgtgcttg gtatggaaat ttttttaaat
      841 ctatacctaa tgtatgaaca attacttttt cagccgggca tccgatttca acaatacgat
      901 ctttaagttc ttgagaatct gccaaaaaca aacttgcttg atgaagtaat tctttatatt
      961 tttttttata taaagcccat tttttttgat gtagttgatc acccagcagt attgcgacat
     1021 catttccatg caaagtaacg atcaaaggta tattcaattt ctttgccaaa ggcaccataa
     1081 aaataccgct gtacgcaaaa tgcgcgtgga tcaggcaaac acggttcctt ataagaactt
     1141 gtttgaatgt ccaactccat cgccaccgat taaacaggta atgttcaata cgatttaaga
     1201 gattatctga aggcaaaaaa ttgataactt catgatcagt aaattgagca gcgtttaagt
     1261 gtttcttagc caaaacaaac ggtttgtaac gttgatgagc atgtaactca tcgtgaatga
     1321 aggtttcaga ataaggtaga aacttggttt taaaaactgc tatccgcggt ggattattca
     1381 tggaatgcgt accagtgtta attagttaat gaccacttaa actaagtgct tattgatgac
     1441 tatgattata ttatcttttt gttttcatac gcttttcttt tgtacacaag agaccgctaa
     1501 atctgagcca tctgcaaatc aatatacgta atgtttataa aacagcttca ttacaataaa
     1561 atctatcgta ttaatattag tagtaaagcg gtcatttaac atatacaaaa catgtgatac
     1621 cttaaagcaa aaaacaatcc gaaacatcta aatattacta ctgtaatcac actcttcaag
     1681 cacgttcgtt atgacgccga gcgagtacga ataaaagcgc tgataaattt tgcacgttca
     1741 gtccacgtgt atttgagacc atccctatat gcatttctgc ttaacgatat gcgcaaatcg
     1801 gaattctctg acaatacttg aaccgctttt ctagctgcgt caaggtcatc gggtcgcacc
     1861 aatatagcgt tttggttatt ctgtaagatc tcgcggcaat ccggcaagtc tggagctacg
     1921 atggcacgcc cggcttgaag atactgaaag gttttaattg gcaaaaccgt gttgcctttt
     1981 ttttcgagac ccgctgctgt agggggaata agcaggatat ctgcaagttg taaataccga
     2041 ctcaccgccg aaggttccac ccaaccggtt cgccgcacgt tggataatcg aagcgtacgt
     2101 attttgttat caaaatctga ttcatctctt tctttttttc cgccacctac aaaaagaaat
     2161 atgatattgg gtagctgttg tgctaaatcc aaaagcatac cacttccttt ggaactgtca
     2221 ctaatacgtc cggcgtaaca gatcaccagt ttcccatcat caatattcaa ttgccttcga
     2281 agtaccattg tttcatttcc atccatctgc cgacccggat gactaccatt ataggcaatt
     2341 gtaattttgg atgatgccag acaaacattt agaaacgatt cggcacacaa cttggagtgg
     2401 gctatcacgc cacaaaaatg gggcctcgta taacagtatt ttctccacaa caggtacttc
     2461 gcacgttggt tgaggttaat ccggtatgtg tcaaagaagg ttttgtgacc agctagtaaa
     2521 ctccataaca gggtttccgg gtgacgggta aaatagaagt caaatttttc tcggcccgcg
     2581 gccaaaattg ccttacaggc gctgtagtgt tttcgccatt tcttcggcag cgtgggcaca
     2641 gttatatatt cgataggaat gtcctgcgca ataccgtaat gttcttgtac attcacattt
     2701 tcgaacccaa cggtcggttt ttctgcaaat actttaacac gccacgacgg attgacgctc
     2761 attattgccg ccacattgtt aataaattgt tgtgagacca catcgttgct gggaataggc
     2821 acccccgcaa taaaagctat actaaataat gtgtgatcgt cgttctgaaa agacatagag
     2881 cgtattgtta ttacgtatcc atataatgga taatattccc tgaatacctt ttgcaaaaaa
     2941 aaatcttggc aggatttaca ctctgccaag attttgattc atttatgata caactaaata
     3001 ttagccgacg tttttcatca tcaacggttg atacaaacca ttggtacgcg catacaatcc
     3061 cgcaggattt gtgtgtaaca taatcgtttg cttcatcagg ccggacattt cgatttccaa
     3121 gtgatgcaga tcttcaaaga aatccagttc accgtatttg aaatctgtta catccaccca
     3181 acctacattt tcacccatat caatcatatg ctgtatagcg cgcacaaaaa atgcattacg
     3241 ggtaacatct ttgatcatgg ccgaatccag tgcctgcttg aacgcctcta taccggaatt
     3301 gcgaaatgcg cgaagcccga tagattcacc gttagcagcg tagttatcaa tctctttact
     3361 gatctcaata acacgatcac cattgatacg cactttcata tcttcattgt cgtaagccgg
     3421 attcttgtga atggcgatac tgacattcat atctttttgc gcaatcactt ttgctaatgc
     3481 cttacgctca aacacattgt caccattgat cacgacaaat tcagttgtca tttcatcttt
     3541 agctaaccaa agtgaaaaaa gattattggt agtagcataa tctttgttat taaccatttt
     3601 gaggcggata tccaaaccat gaatcgcaat ataacgctct gcatagtttt tcaatttttc
     3661 gatctcaaac ccgccgatga tcacaacatc tttgacaccg cagctggcca acgtaaccaa
     3721 ctggtattcc agtatcgttg tgcctttgac atctaccaac gttttaggtg tctcagccgt
     3781 aagaggaagc aaccgcttgc cttgacctgc cgctaagatg aatgctttca tttgatatct
     3841 ccagttctct tattttctac tttttaaacg ccacttcatt cacagtgaca ttcatttatg
     3901 tagcaatctg cgtgccgaaa tattaaaaaa aagaaagtta ctatgacgtg ttgtttttta
     3961 ttaatttact ttaatattgt taattagttt ttaaaaatta ttatcgaatt gctgcatgta
     4021 cgagaaataa ggaacttgca ggtgcaataa aaaacttgca tttcgaagtt caaatagatg
     4081 tatttgtgtg atttaagaca cacttatcgg gttattaaaa agaaacagac ggtattgggg
     4141 ctgcgaagaa gaagaatggg aagtatttga aaagagagaa cagaactcaa catgctgcaa
     4201 agttatataa ccttattata aaaaaatatt tcgcgattaa cgatagccat tgatgtaatt
     4261 ctagtaatga agaataatcc ggttttatga gagaccgcat ttaatgcaat accctatcag
     4321 cgtaattgtg gataacgtac taaatttaac agccgatgat attgagttgg ggtgctctcg
     4381 gttacccaaa acaacaacaa attaagatta aatcatagag attgatcagg cacaataaaa
     4441 aaacagctat ttagaaaatt ccatcgcgtt ggtagaaatc agattggtcg gtagactatt
     4501 tcttcccttt ttgcgccaat aaatttcaag taacaaccta tcattgtgac gcgccatcac
     4561 atccaaggag aaattattta tcgtccattc acgcccttgt ctccccaaaa cggcggctgt
     4621 tgcaggacag atcagaagtt ttttcatggc ctcgctcaga ttctccacat cgtctggcgc
     4681 gataagtaaa ccactgtaat tgtctcgaat ggcctcagta attcccccga cccttgtacc
     4741 gataaccgct ttaccgcgca taccggcttc gacaaaaact aatccgaagc cttcaacaaa
     4801 ttcacgtgtc actcgacccg gcatagtaaa aatatctgcc actgcataat aattttctaa
     4861 aagtgaggac tcaacataac ccgcaaaaaa cacataattt tccaaaccta attttttgac
     4921 cagcatttca agatttttgc gagtatttcc ttcaccgact atgatataaa gaatctcggg
     4981 gatttcgctt cgcaaacgat gaatagattg aattaccaaa tcatgtcctt tggcctccac
     5041 aagacggcca accgtcaata ctattttacg attgcgccat tgcgggcaaa acgttagggc
     5101 gtcctctttt gttttcacaa ccgccggatt aaattctatt ccgttgtata tgactttcag
     5161 tttggattgc aatattttat cggacacaga attacgtatc aatttttcgg cttcgttact
     5221 aatcgtaaca atagcatcag cgcgtcgaat aaataagccc ccgagccacg acgatagttt
     5281 tacaaaaaaa tttcgtggct catctgagcg caatataccg cccccatgga ttgtgccaat
     5341 ccagcgacca ggcaaaaaag ccaacacggc tgtggagcga caaccggcaa acgtcgagct
     5401 ccagatcaca tccgggcgaa attttaaata atgtgttata atagctatcg tcgcaaaaat
     5461 aattcgtatt ccgtaccaca cttcattggt taacggcata cgcacgattt cataatcaga
     5521 actttcagat atgttttttc gtttatattt cggagccaac aaacgaactt gatgaccttg
     5581 atgatgaagc ccttgtgcaa gatgatgcgc ataggtagca ctgcccccgc tggcaggaaa
     5641 aaatttcatc gctacgatga gaattcgcat acgactccaa ttaggttata attagtagtt
     5701 attaaaaata caagcttgat agagagagaa taaaacctaa aaattaggca tccattaaat
     5761 taaattttca tgagcaacgt taaaccatta acctattccg acagttataa aacccctgca
     5821 aagcaaacaa cacaaatatc tagtgggttc ttaaaatttt ttacacttat tagcaactca
     5881 gccgggtttt agtcacgcca atggcgaagc actatcctaa ttatttttta cttagagaca
     5941 attggcggtg atcaataagc cactgcgctg attgcataat tgatttcaaa tattcggaca
     6001 tccttgcaac tacggcttgc gaatctggga ctgctatatc cattgacaat cgtgatacga
     6061 caggtttatt ttttggagta agcgacaata aaaaggattt ggacaaacac cctactttgg
     6121 tgtctgaatt aataggcaaa aactccctcc gttcacgcag taagtcaatt ccaaatgtgt
     6181 tatttgtata atctccgccg attagcccca tgatcgttgc cggtatatct atttgtccgc
     6241 caagatcact tataatacta ggtggaaggc ccttactcca aataataaat ggaatgtgaa
     6301 ggtattcgag atccatatca tacgtgttta cgccgcgcgc cccgtgatcc cctaaaaaaa
     6361 caaagatcgt acgatcaaac cacttggatt tttttactga cgccataaat gaaccgatac
     6421 tttgatcggc aaaacgtacc gcatgcgatc tatccttttc gccgggataa ggtgaaaaat
     6481 catccggtat tgcatagggt ccatgattgc tcaccgttag tatgacagag aagaaaggcg
     6541 aatgatcagc caatgtatcc aatactttgc cggcagactg aaaaagacta cgatcatcca
     6601 caccccacag gttagccgtc tccgcatcac gataatcgga cggatcataa acacgatcga
     6661 acgcattcat ccccaaaaaa ccaccgatat tatcaaacgt agcttgatgc ggtgagaaaa
     6721 agaccgtacg ataacctcgc tcttttagca tgtgactaag tcctgcatgt tcttgtaagg
     6781 cttgagtcgt attcatcgga tgctgtttga atatggccgg caacccaaaa agtgtagaat
     6841 gcacaccatt ataagtatgt attccagaag agtaaaaatt attaaacaac aacccctgcg
     6901 cagcgattga atccaaatgc ggggtcaaat tctctttatt ccccaacgca cctacaaatt
     6961 ttgacgccat tgattccatt aggacaatta cgatgttttg atctgatgcc ggcttgatac
     7021 cgcgtatttc gtgcgcaagc aaacgggtcg aactgcttcc ggattccgga taataccgat
     7081 gtagcacggt aaaagcctct tgttcagaca tccaattaat ttttttggat ccacgaaatg
     7141 ccatccgcca agcatccttg gtaaaaccgt taaccgggtt gatcgccgca gtattaagaa
     7201 aaacattgtt tgaaaaaaaa gcggtggcgg acccttctct aaactttcga tgccatgcaa
     7261 aaccacgtga cgcctgaaac cacaaaccaa cgatcatcag cgcaatcaaa atcgaagctc
     7321 gatttaatct cggtttagag gctattacga ctgagtatct attaaacccg aactgcatta
     7381 agcgcacgaa aagataactg agtaaaatcc atatgacgac aagcaatgcg tactgtgaat
     7441 acaacatcga aaaaacaaca tccgaagacg acattaggtt gaataatgat gcatttaggc
     7501 gtgaaccaaa ataaccatag tacccccaat cggcaatgac gactgcactg atatagcata
     7561 ctgtcacata tacacatatc ctgaaaaatc tttccgtacg agttaacgat acgccccaaa
     7621 acatgcgaac caataataga agcgttggaa tccctgtcag ataagctata ttgattatgt
     7681 cgttttttcc gccaatccaa aataattgcc caatatctgc gtagctattc atttggcctt
     7741 catcaaattc atatataaat gcagcgcgag caagcgtaaa aaaacataaa aaaaccagcc
     7801 aaaaaaaact taaaaaccga agcgttgcta ccacatccct ctcattcata gagctttctt
     7861 cccttattta aaaacctata ttatatgatt tactacgaat gtatattatt tattattagt
     7921 attgttcaga cattgtgagt ataatttttt taattaaaaa gaaaaacaag ttttcaaagg
     7981 caaatattat accaacaagc aaaactatgt tttttaaatt cagcgctttt cattttaatt
     8041 caaacttatg atctcatttt tttatgctta tgattatact gattttcgct ccttgatttt
     8101 tacttcatct gaaaaaccgt cactcagaat tcttcgagtc cgtctcgtta cgtttattaa
     8161 aaattttcac actgccatat tttaattgca cacaccatga ttaatttttt tcgttctatc
     8221 ttctcctcaa aacccaagcc tccggtagca gccataaagc cgcatcatgt aacgcatata
     8281 ggtaaatcac ttacagacga ttattattgg ttgcgcgaca aagaaaatcc ggaagttatt
     8341 gcctatctga gagctgaaaa tacatataca gacgccttga tgaaacctac tgaggctttg
     8401 caagatacgc tgtatcgcga aatgctcagc cgcattcggg aggacgaccg atccgtccct
     8461 gaacaaatcg gtgagtttct atattattac cggacggaga aggacaagca gtataaaatc
     8521 tattgccgca aaaaaaacgg cgaggaaaac gaactcgtac tactcgacct caacactctg
     8581 gcccggcata caccctatct tgcactcggc gcctaccgtg tcagtccgga tcaccattgg
     8641 ctcgcattta cgatagatca tgacggatca gaacaattca cactttacat caaaaatcta
     8701 gaaaccggag aattatcccc cgaacacatt ccgaatacgt attacggttt ggagtgggcc
     8761 aacaacagtg caacgatttt ttatacgacg cttgatccaa gccttcgccc gcatcgggtg
     8821 tatcggcaca gtatcggcgc agaattaaca tccgatcaac ttgtgtacga ggaatccgat
     8881 gaacggtatt atcttgacct taccaaatcg cgcagcgggc gttttctttt gataacattg
     8941 gccagtaaaa caacaaccga agtgcatttt ttggatgcgg atcatcccga caaaccgttt
     9001 caagtatttc aggaacgtgt gcaaggtcat gagtataacg tcgaacattg ggaggatcgc
     9061 ttttttatca cgaccaatga tcaggctgtt aattttcg
//
LOCUS       DAOQGF010000124         9033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3634_length_9033_cov_6.720316, whole genome shotgun sequence.
ACCESSION   DAOQGF010000124 DAOQGF010000000
VERSION     DAOQGF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3634_length_9033_cov_6.720316"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK937_13395"
     CDS             <1..617
                     /locus_tag="PK937_13395"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH33615.1"
                     /translation="TGNGQAAIRLAESFDTVTATDASAQQIRQAQPHPKVHYYVEPAE
                     QSHLETGSVDIITAAASIHWFDMHAFYGEVKRVARPDALLAVWAYDRVKISNEIDILV
                     DRLYYDIAGPFWPKERRWVEEQYKTIPFPFEEISAPSFAINHTLTVDDLIGYLRTWSS
                     VQGYLKVHGKDPVDWITGELHNLWGSEKRPVEWPMFMRIGRIHK"
     gene            637..1440
                     /locus_tag="PK937_13400"
     CDS             637..1440
                     /locus_tag="PK937_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HNH33616.1"
                     /translation="MVQISDLRIYPIKSCKGFSVPSVTLDAYGFTDDRRFMIVDTSGR
                     FVTAREVNPLLWIEPSIESGILYMRAPGMELFATPIHLMPQSIVRVTVWEDTCEAWDC
                     GGAPAEWLSQFLGARYRLVRMQPQFLRPVDRKYAVADDHVSFADAYPALVISEASLEL
                     LNSKLASPVTMTRFRPNVVVSGCAAHEEDTWQIMRTGAVEFRGVKRCSRCVLTTVDPE
                     TTESGAEPLKTLATYRRSAEGKIMFGMNLIPVQKNGKLRIGDTITYTGS"
     gene            1443..2222
                     /locus_tag="PK937_13405"
     CDS             1443..2222
                     /locus_tag="PK937_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNH33617.1"
                     /translation="MKITMYQVDAFTRKVFSGNPAAVCPMQVWPEDEIMRGIAAENNL
                     SETAFFIPQGDDFALRWFTPEVEIDLCGHATLATAHVLFEHLHFERSEIHFHTQSGKL
                     TIRRLPDGKLLMDFPARKPLPCTVPRVLVEGLGANPDVILKARDYFCVFDSEATVRAL
                     SPDFTMLKSLGEKVIVTARGDTTDFVSRFFAPSVGINEDPVTGSAHCSLIPYWAERLG
                     KDELYAHQVSKRGGEIFCKLRGDRVDIGGYAADYLQGTIQW"
     gene            2228..2653
                     /locus_tag="PK937_13410"
     CDS             2228..2653
                     /locus_tag="PK937_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH33618.1"
                     /translation="MRIHFITLGVSDMQRAANFYSDVLGFKILKKTPVITFFDNGGMV
                     LGVYPIQQLNALTGASFETVSPSSTGILLSHNVQNENDVDAWVNKLKNEKVKILKEAQ
                     PAVWGGRHAFFSDPDGYSWEICWNPKLIPDEFEHLPLVN"
     gene            2716..3057
                     /locus_tag="PK937_13415"
     CDS             2716..3057
                     /locus_tag="PK937_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33619.1"
                     /translation="MLPLEGLIAWAVFIGMMVIAFKLAILVMIFVVMQSRQNQQRWRE
                     HRSFGYLYSCLVCFLIGSVGTMYMFTVSDKAELTFLDFYVSPIIFSVCCVLYFWIGYV
                     WKKKIQQPKAP"
     gene            3067..4602
                     /locus_tag="PK937_13420"
     CDS             3067..4602
                     /locus_tag="PK937_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNH33620.1"
                     /translation="MVFELNKESDVPLYTQIERRMTELIRVGILKADEKLPATRDLAL
                     QLHVHRNTVVQAYQELEVKGFVYSEVGRGTFVSKHAHVAAKGTVAAQNKSGNFSFDGL
                     YAEHWINPEDPSMQAIDTIVRTCSKPQDMIAFSSVVPDKHFFPLVDFQNCSYRAIQKY
                     GVDLLEMGDTQGFRPFVEYLPKFLVRRGLTVQSSDITMVSGIQQGIDLIARILINPGD
                     TVITEEVTYHGARRIFKSLGANIIGIPMDDQGMRMDILETILARQKVKMIYTIPTFQN
                     PTGRTMPLDRRKHLLELAYHYEIPIVEDQYANELRMEGEEIVPIAALDDEGIVIALGS
                     FSKILFHGIRLGWVITSNRAFQNKLNYAKRLTDWQNNYLIQGAILEFCEAGHFDRYLK
                     RKMKVVKERRDAMITASRQYFPEEVRFHVPAGGLFEWADLPDGMDEYDVLMEARQRRV
                     LFTPKRFFSVRPDAGGGMRLGFVSQTTEHIHEGIAILGDIIKKKIGSAPWVESDSALA
                     SVM"
     gene            4610..5071
                     /locus_tag="PK937_13425"
     CDS             4610..5071
                     /locus_tag="PK937_13425"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH33621.1"
                     /translation="MMNTKDFIKRWQYEIWADRLVSDSIMALPAGRGRASQLFSHLIE
                     SKVLWYQRIHGEHKHITTVWSDLPITRCLDVLSEINSNWDKFVHELSEEVLQRTITYK
                     TMNGQPYQSTVHDILTHILNHSTYHRGQIAMEVRKAGGTPAQTDYITFCRE"
     gene            5077..5568
                     /locus_tag="PK937_13430"
     CDS             5077..5568
                     /locus_tag="PK937_13430"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNH33622.1"
                     /translation="MQGIIKQINISSGGIPKKPTASAYVSSERVQGDDWNNKNVHGGP
                     RQVVLMIANETYQSLVQLGYPVFPGALGENFTTEGLAYAPIRFGDTFLVGGKLILRIT
                     KLRVPCNTIKVYGADIGTHLYDALARDGNPESPHWGTGGFYAEVLQEGTVQAGDVIRI
                     KKD"
     gene            5605..6525
                     /locus_tag="PK937_13435"
     CDS             5605..6525
                     /locus_tag="PK937_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH33623.1"
                     /translation="MSSHKHYWPHIAIAICTLIWGSTWLAIKIGLEDLPPFLFAGIRF
                     VIASVLLLALMFFQRIRFPRDRHSWHVMLFLGFFQMLDYAFVFWGEQYIDSGIGAIIF
                     ATMPFFVIILGYLWLAEHKITGVKIFGTVVSFAGVTVVFLRDIHHIGTSWMGDVSIIL
                     AALCGAVISVYAKKHADGIHPVVNVTVQMVICAVCLLAAGLLTEDTSRLHWTPQSVAS
                     IFYLGVFGSAVAFVVYMWVIKKVSVVEAAIIPVATPLVAVILGWLVRGEALTVRAMIG
                     CVLILIGVYFVNVYRSRARTAVQTIVEDHN"
     gene            6611..7492
                     /gene="pdxS"
                     /locus_tag="PK937_13440"
     CDS             6611..7492
                     /gene="pdxS"
                     /locus_tag="PK937_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNH33624.1"
                     /translation="MLKGTFKVKVGLAEMMKGGVIMDVVNPEQAKIAEDAGACAVMAL
                     ERVPADIRRDGGVARMSDPEMILGIMDAVSIPVMAKARIGHFVEAQILESLGVDFIDE
                     SEVLTPADEANHIYKHDYKVPFVCGATNLGEALRRIGEGAAMIRTKGEAGTGNVVEAV
                     RHIRTIIGHIKKLTVMGTDELMSEAKTMGAPFELLQWVAKNGKLPVPNFSAGGIATPA
                     DAALVMQLGAEAVFVGSGIFKSGNPVKRAEAIVKAATHFKDPAILAEVSKSLGAAMVG
                     INVDNLAASELLANRGW"
     gene            7512..8027
                     /locus_tag="PK937_13445"
     CDS             7512..8027
                     /locus_tag="PK937_13445"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH33625.1"
                     /translation="MKDKIEKLRRVHRVARAGLIKTLDGITEEQLYWQPAAESRSIGD
                     MMRHLIRVDMWFYKRHDIAPDVADIKRPTVTQLQTMLQQALGQYEAILDGLESDALLD
                     RRTTATDKVPYKSLGAVMAHIPQHYLYHMSQMIYLRRAQDRTWESPLPEWETATDVIA
                     QYMVGMAGEHE"
     gene            8044..8625
                     /gene="pdxT"
                     /locus_tag="PK937_13450"
     CDS             8044..8625
                     /gene="pdxT"
                     /locus_tag="PK937_13450"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNH33626.1"
                     /translation="MQKKIGVLALQGDFEAHQKMTQSVGADAVQVRKPSELEGLHGLI
                     IPGGESTTLIKLMRAFAMDDAIKAFYDKGGAIFGTCAGSILVAKTIVNSPQFRFGLID
                     ITVERNGYGRQVDSFEKDITIPVLGEKSVHAVFIRAPRIVAAGPATEILSSVGENILA
                     ARSGRVLVCTFHPEITDDARMHRYFVEKVVGTK"
     gene            8667..>9033
                     /locus_tag="PK937_13455"
     CDS             8667..>9033
                     /locus_tag="PK937_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33627.1"
                     /translation="MRSLFKKIINHYRVPDDAYETFRHDFKYGISKGLVLAGSAGVAG
                     VILKKSFSASDFQVALLYSATNLGLLFSIFTAVHAERANKMNYAYVPDAIARTLFIFT
                     AVATALIPNAWLFTAILCAA"
BASE COUNT         2269 a         1997 c         2392 g         2375 t
ORIGIN      
        1 gcaccggtaa tgggcaggcg gcaatcaggt tagcagaatc ttttgatact gttacggcga
       61 ccgatgccag cgcacagcag attcgccaag cgcaaccgca tccgaaagtt cattattatg
      121 tagaacccgc agaacaaagt catttggaaa caggtagtgt ggatatcatc acagcggccg
      181 catccattca ttggtttgac atgcatgcgt tttacggcga agtcaaacgt gtagctcggc
      241 cggacgctct tctcgcagta tgggcgtatg atcgtgtaaa gatttctaac gaaatagata
      301 tcctcgtaga tcgtttgtat tacgatatag ccgggccatt ttggcccaaa gaacgacgct
      361 gggttgagga gcaatataaa acgataccgt ttccatttga ggaaatttca gcgccttcct
      421 ttgctatcaa ccatactctg actgtagatg atttgatcgg ctacttgcgt acatggtcca
      481 gcgtgcaagg ttatttaaaa gtacacggca aagatccggt tgattggata accggtgaac
      541 tgcacaatct ttggggttcg gagaaacgcc cggtcgaatg gccgatgttt atgcgcatcg
      601 ggcgtattca caaataacat taactttaaa aaaaaaatgg tacaaatcag cgatcttcgc
      661 atttatccga tcaaatcctg caaagggttt tccgttccgt cagttacgtt ggatgcgtac
      721 ggatttaccg atgatcggcg ttttatgatc gtggatacat ccggccgttt tgtgacggcg
      781 cgtgaggtga acccattgtt atggatcgaa ccctcgatcg aaagcggtat tttgtatatg
      841 cgggcgccgg ggatggagct ttttgcaacg ccgatccatc ttatgccgca aagcatcgtc
      901 cgcgtcacgg tatgggaaga tacgtgcgaa gcatgggatt gcggcggcgc accggccgaa
      961 tggctgagcc agtttctcgg tgcgcgctac cggttagttc gtatgcaacc acagttttta
     1021 cgaccggtgg atcgcaagta tgccgttgcc gatgatcacg tgagttttgc cgatgcgtat
     1081 cctgcgctgg tgatttcgga agcctcgtta gaattactca attccaaact ggcttcgccg
     1141 gtgacgatga cacgatttcg cccaaatgtc gtcgtaagcg gttgcgcggc gcatgaagaa
     1201 gatacatggc aaatcatgcg taccggtgca gtagagtttc gcggcgtcaa acggtgttcc
     1261 cgttgtgtac tgacgacggt ggatccggag acaaccgaaa gcggtgcgga acctttgaaa
     1321 acactggcga catatcgccg gagcgcggag ggtaaaatta tgttcgggat gaatcttatt
     1381 ccggtgcaaa aaaacggcaa actgcgtatc ggcgatacga tcacttatac gggatcataa
     1441 aaatgaaaat cacaatgtat caggtggatg cgtttacgcg taaagttttt tccggcaatc
     1501 cggcagcggt ttgcccgatg caagtctggc cggaagatga aataatgagg ggtattgccg
     1561 ctgaaaacaa tctttcggaa acggcgtttt ttattccgca aggtgacgat tttgcattgc
     1621 ggtggtttac cccggaagtg gaaattgatc tttgcggtca tgccacactg gcgacggccc
     1681 atgtgctttt tgaacacctg cactttgaac gatcggaaat acattttcac acgcaaagcg
     1741 gaaaattgac catacgtcgt ttacctgacg ggaagttgct tatggatttt cccgcgcgca
     1801 aacccttacc ctgcacggtg ccgcgggtat tagttgaagg tcttggcgcc aatccggacg
     1861 taattcttaa agcacgcgat tacttttgcg tatttgactc cgaagcgacg gtgcgtgcat
     1921 tgtcgccgga tttcacaatg ctaaaatcat taggcgaaaa agttatcgtc acggcgcggg
     1981 gcgatacgac ggattttgtg tctcgatttt ttgcaccgag cgtcggtatc aatgaagacc
     2041 ctgtcaccgg ttcggcgcat tgttcgctga tcccgtactg ggcggagcgg ctgggaaaag
     2101 atgaattgta tgcacatcag gtttcaaaac gcggcggtga aattttttgc aaattgcgcg
     2161 gtgatcgggt tgatatcggt ggttatgcgg ccgattatct gcaaggtacg atccaatggt
     2221 gaaaccgatg cgaatccatt ttataacact cggtgtgtcg gatatgcaac gtgcggcgaa
     2281 tttttattcg gatgtattgg gattcaaaat attgaaaaaa acaccggtga ttacattttt
     2341 tgacaatggc ggtatggtac tgggggtata tcccattcaa caactgaacg ccttaaccgg
     2401 cgcgtcgttt gaaacggtat cgccttcgtc aacgggaatc cttttatcgc ataatgttca
     2461 aaatgaaaac gatgttgatg cgtgggtcaa taaactaaaa aacgaaaagg taaaaatact
     2521 caaagaagca caacctgccg tatggggcgg gcgccatgct tttttttccg atccggatgg
     2581 gtatagctgg gaaatttgtt ggaatccgaa attaattccc gatgaattcg aacatcttcc
     2641 gctggtaaat tgaacgcatg cgatgagatt tagttatgtt aatacagcca atattcacta
     2701 aaaatttata attctatgtt accactcgaa ggcctcattg cgtgggccgt ttttatcggt
     2761 atgatggtca tcgcttttaa gttggcgatc cttgtgatga tttttgtcgt tatgcaaagc
     2821 cggcagaatc agcagcgatg gcgcgaacat cgttcgttcg gctacctcta cagttgttta
     2881 gtatgttttt tgatcggttc ggtcggtacg atgtacatgt tcaccgtaag cgataaggcg
     2941 gaattgacct ttcttgattt ttatgtcagc ccgatcattt tttccgtgtg ttgcgtactc
     3001 tatttctgga tcggttatgt ttggaagaaa aaaattcaac aacccaaagc tccataaagg
     3061 tgatgtatgg tgtttgaact caacaaagaa agcgatgttc ctctttatac gcagattgaa
     3121 cggcgtatga cggaattgat ccgcgtcggg attctcaagg ccgacgaaaa attacccgct
     3181 acgcgtgatc tggctttaca gttgcatgtg caccgtaata cggtcgttca ggcgtatcag
     3241 gagcttgaag ttaaaggttt tgtgtattcc gaagtaggtc gcggtacgtt tgtaagtaaa
     3301 catgcacacg ttgcagccaa agggacggtc gctgcccaaa acaaatccgg taatttttca
     3361 tttgacggtt tgtatgcgga acactggatc aatcccgaag atccttcgat gcaggcgatt
     3421 gatacgatcg tgcgtacatg cagcaaaccg caggacatga ttgcgttttc ttcggttgtg
     3481 ccggataaac atttttttcc gctcgttgat tttcaaaact gttcctatcg ggcgattcaa
     3541 aaatacggag tggatctgct ggagatgggt gatacgcagg ggtttcgacc gttcgtcgaa
     3601 tatttgccga agtttcttgt gcggcgcggg cttaccgtgc agagctcgga catcacgatg
     3661 gtcagcggca ttcagcaagg catagatctg atcgcacgga tcctgatcaa tccgggcgat
     3721 acggtgatca cggaagaagt aacctatcac ggtgcgcggc gcatattcaa atcgctcggc
     3781 gccaatatca tcggcattcc catggacgat cagggtatgc gcatggatat tcttgaaacg
     3841 atactcgcac ggcaaaaagt aaaaatgatt tatacgatac cgacattcca aaatcctacg
     3901 ggtcggacga tgcctctgga tcggcgtaaa catcttttgg aactggcgta tcattacgaa
     3961 ataccgattg tcgaagatca atacgccaac gaactgcgca tggagggcga ggagatcgtg
     4021 ccgattgcag cactagacga cgaaggtatt gtcatcgcgc ttggcagttt ttcaaaaata
     4081 ttatttcacg gcattcgtct gggttgggtg attacttcca atcgcgcgtt tcaaaataaa
     4141 ctgaattacg ccaaacgtct cacggactgg caaaacaatt atttgattca gggcgcaata
     4201 ttggagtttt gtgaagccgg gcatttcgat cgctatctga aaagaaaaat gaaagttgtc
     4261 aaagagcggc gcgatgcgat gatcaccgcg tcccgtcagt actttccgga ggaagtgcgt
     4321 ttccatgttc ctgccggagg tctttttgag tgggcggatt taccggacgg tatggatgag
     4381 tacgatgtac tgatggaagc ccgtcagcgg cgtgtgcttt ttacgcccaa gcggtttttt
     4441 tctgtgcgcc cggatgccgg tggcggtatg cgtttgggtt ttgtttcgca aacgacggaa
     4501 catattcatg aaggtatcgc gatactcgga gatattatta agaaaaaaat aggcagtgcg
     4561 ccgtgggtcg aatcggactc cgcactggcg tcggtgatgt aggataatta tgatgaacac
     4621 aaaagacttt attaaacgct ggcagtacga aatctgggcg gatcgtttag tatccgattc
     4681 catcatggct ttaccggcag gtcgaggccg tgcgtcacaa cttttttcac atctgatcga
     4741 atctaaagtt ctatggtatc agcgaattca tggcgagcat aaacatatta ccacggtatg
     4801 gagtgattta ccgatcacac ggtgtttaga tgttctttca gaaataaatt caaattggga
     4861 caagtttgtt catgaattga gtgaggaagt tttgcaacgt acgataacgt ataaaacaat
     4921 gaatggtcag ccctatcaaa gcaccgtgca tgatatttta acgcatatac tcaatcactc
     4981 gacctatcat cgcgggcaga tcgcgatgga agtgcgtaaa gccggaggaa cgccggcaca
     5041 aaccgattac atcacatttt gcagagaata atccgtatgc aaggtatcat caaacagatc
     5101 aatatttcgt cgggcggcat ccctaaaaaa ccgaccgcat cggcgtatgt cagtagcgaa
     5161 cgcgtacaag gtgatgactg gaataacaaa aatgtgcacg gcggaccgcg ccaagtcgtc
     5221 ctgatgattg cgaacgaaac gtatcaatcg ttggtccaac tcggttatcc tgttttcccc
     5281 ggcgctttag gagaaaattt tacgacggag ggattagcgt acgcgccgat tcgttttggc
     5341 gatacgtttt tagtcggagg aaaacttata ctacgcatca ccaagctgcg ggtgccgtgc
     5401 aatacgatca aagtatacgg agcagatatc gggacgcatc tgtacgacgc tttggcccgg
     5461 gacggtaatc cggagtcgcc gcattgggga acgggcggat tttatgccga ggttttacag
     5521 gaaggtacgg tacaagccgg cgatgtgatc cggataaaaa aagattaaat agaaaaaaat
     5581 acatagaaag tatacgaggt tcttttgtcg tcacacaaac attactggcc gcacatcgca
     5641 attgcgattt gtacattgat ctggggttcg acctggctcg ccatcaaaat cggtcttgaa
     5701 gatttaccgc cgtttttatt tgccggaatt cgttttgtta tcgcatcggt tttgttactt
     5761 gcgctcatgt tctttcaacg tattcggttt ccccgcgacc ggcattcgtg gcatgtcatg
     5821 ttgtttcttg gtttttttca aatgctggat tatgcctttg tattctgggg tgaacagtat
     5881 attgattcgg gtatcggtgc gattattttt gcaaccatgc cgttttttgt catcatactc
     5941 ggatacctgt ggctggcgga acataaaatc acgggagtca aaattttcgg taccgtggtt
     6001 agttttgccg gagtcacggt tgtttttctg cgtgatattc accatatcgg tacgtcatgg
     6061 atgggcgatg tttccatcat tttagcggcc ttgtgtggcg ccgttatcag cgtgtatgcc
     6121 aaaaaacatg cggatggcat tcatcccgtc gtcaacgtaa cggtacaaat ggtcatctgc
     6181 gccgtttgcc ttttagctgc agggttactt accgaagata cgtcgcgtct gcactggaca
     6241 ccgcaatccg tcgcgagcat tttctacctc ggcgtattcg gctcggcggt cgcctttgtc
     6301 gtgtatatgt gggtgatcaa aaaagtttcc gtcgtggaag cggcgatcat acctgttgct
     6361 acgcccttag tcgccgtgat actcggctgg cttgtccgcg gagaagcgtt gactgtacgt
     6421 gcgatgatcg gctgcgttct gatactcatc ggtgtgtatt ttgttaatgt gtatcgttcg
     6481 cgcgcacgaa ccgctgttca aacgatagtt gaggatcata attgaccctg aattaaaaaa
     6541 aattatattt gcgcacttaa aaaatcgaaa ggaatatatc atgacagcaa gtacacaagg
     6601 agaagacaat attctcaaag ggacattcaa agtcaaagtc ggtttggccg agatgatgaa
     6661 gggcggcgtc attatggacg tggtcaatcc cgagcaggct aaaatcgccg aagatgcggg
     6721 cgcatgcgcc gtgatggcgt tggaacgcgt tccggcagac attcgtcgtg atggcggcgt
     6781 ggcgcgtatg agcgacccgg aaatgatact cggtattatg gatgcggtca gtattcccgt
     6841 catggccaaa gcacgcatcg gccactttgt cgaagcgcaa atcctcgaat cgctgggtgt
     6901 ggattttatt gacgaaagtg aagtacttac gccggcggat gaagctaacc acatatacaa
     6961 acatgattac aaagtacctt tcgtttgtgg tgcgaccaat ctcggcgaag cgctgcgtcg
     7021 tatcggcgaa ggggcggcga tgattcgtac caaaggcgaa gcgggtaccg gaaacgtggt
     7081 cgaagctgtg cgccatatcc gcacgatcat cgggcatatc aaaaaactga ccgtgatggg
     7141 tacggacgaa ctgatgagcg aagcaaaaac gatgggtgca ccgtttgaac tgctccaatg
     7201 ggttgcaaaa aacggcaaac tgcctgtacc caatttttca gcgggcggta tcgctacgcc
     7261 tgccgatgcc gcactcgtga tgcaactcgg cgcggaagcg gtatttgtcg gatcgggcat
     7321 tttcaaatcc ggaaacccgg tcaaacgtgc ggaagcgatc gtcaaagcgg cgacgcattt
     7381 caaagatcct gcgatattgg ccgaagtgtc gaagtcgctc ggcgcagcga tggtcggcat
     7441 caatgtggac aatcttgccg ccagtgaatt gttagccaat cggggatggt aaccgatcac
     7501 cggatataag tatgaaagat aaaatagaaa aacttcgccg ggtgcaccgc gtcgcacgcg
     7561 caggcctgat caaaacctta gacggtatca cggaagaaca gttgtactgg caaccggcgg
     7621 ccgaatcgcg ttcgatcgga gacatgatgc gtcacctcat tcgcgttgac atgtggtttt
     7681 acaaacgcca tgatatagcg ccggatgtag ccgacataaa acgcccgaca gtcacgcaac
     7741 tccaaactat gttgcaacag gcgttggggc aatatgaagc cattctcgac ggattggagt
     7801 ctgatgcgtt attggatcga cgaaccacgg cgacggacaa agtgccgtac aaatcgttgg
     7861 gcgctgtgat ggcgcatatc ccgcagcatt atctttatca tatgagccaa atgatatatc
     7921 tccggcgcgc acaggatcgc acctgggagt cgcctctgcc ggagtgggaa acggcgaccg
     7981 atgtgatcgc gcagtatatg gtcggcatgg ccggtgaaca cgaataatta agagatcaag
     8041 tttatgcaaa aaaaaattgg tgtgctggcg ttgcaaggtg attttgaggc acatcagaag
     8101 atgacacagt ctgtcggcgc cgatgccgtg caggtacgca aaccgtccga actggagggc
     8161 ttgcacgggt tgatcattcc cggcggtgaa agtaccacac tgatcaagct gatgcgcgca
     8221 tttgcgatgg atgatgcgat aaaagcattt tacgacaaag gcggcgcaat tttcgggacc
     8281 tgcgcgggtt cgatacttgt tgccaaaacc atcgtcaatt cgccgcagtt ccgcttcggg
     8341 ttaattgata ttaccgtcga gcgtaacgga tacggacggc aagtggatag ttttgaaaaa
     8401 gacatcacga ttcctgtttt gggtgaaaaa agcgtgcatg ccgtgtttat tcgtgcaccg
     8461 cgcatcgtcg cggcaggccc cgcgacggag atcctgtcgt ccgtcgggga aaacatattg
     8521 gccgcccgtt ccgggcgtgt actggtttgt acatttcatc ccgagatcac cgatgatgcg
     8581 cgtatgcatc gttatttcgt ggaaaaagtc gtcggtacaa agtgaatttc ttttgttaaa
     8641 gtttgtattc ggatagatgg tatcgtatgc gttcattgtt caaaaaaatc atcaatcatt
     8701 atcgtgttcc cgatgatgcc tacgaaacgt tccgtcatga ttttaagtac ggtatttcca
     8761 aagggctggt gctggccggt tccgccggcg tggccggtgt tattctcaaa aaatcatttt
     8821 ctgcttccga ttttcaagtc gctttgctct atagtgcgac caatctagga ttgttgtttt
     8881 ccatctttac ggccgtccat gccgaacgtg ccaataaaat gaattatgcg tacgtgccgg
     8941 atgccattgc gcgtaccttg tttattttta cggccgtggc tacggcgctg atccccaatg
     9001 catggctttt tacggccatt ctctgcgcgg cgt
//
LOCUS       DAOQGF010000125         8959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3670_length_8959_cov_5.678571, whole genome shotgun sequence.
ACCESSION   DAOQGF010000125 DAOQGF010000000
VERSION     DAOQGF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3670_length_8959_cov_5.678571"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..639)
                     /locus_tag="PK937_13460"
     CDS             complement(343..639)
                     /locus_tag="PK937_13460"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNH33628.1"
                     /translation="MATNTTKADIIHRIAEATGITRFDTDAVINAFIAVIHEELQQGR
                     EIEIRGFGSFRIARRPARTARNPKTGAAVDLPERRVPQFRPSKELKKSVNEIRS"
     gene            complement(644..1531)
                     /gene="sppA"
                     /locus_tag="PK937_13465"
     CDS             complement(644..1531)
                     /gene="sppA"
                     /locus_tag="PK937_13465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNH33629.1"
                     /translation="MKNRSTWILLLGFLSCFVGLIIYVVYRVAEPDVNFGRRIALIEL
                     EGTIVDSRDIVRQFKKYRDDSSVEAIVFRIESPGGGITPSQEIFDIVKKTKAAGKKII
                     VSMGSVAASGGYYVACPADTIVANPGTITGSIGVIIQFPNVDKLLDKIGVDFTTIKSG
                     AFKDILSPYRPMNPSESEWMQSFVMNAYGQFVRAVADERGLDTAYVQQYADGRVYTGE
                     QALQIKLIDVIGSYDDAIRLAADMAGIEGTPNLVKEKKHKPSFFDLLFEEDAESKIES
                     ALSIPGFKSYPVVSYKYGI"
     gene            1857..2777
                     /locus_tag="PK937_13470"
     CDS             1857..2777
                     /locus_tag="PK937_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33630.1"
                     /translation="MKQEDYSTTNLNLRSEILQISGLSVLILLLLLFLGGCSISGDDF
                     TDTVQDEIKQIIKEDEQLAYGVEVVNDSSEITAPQKSAASESALVELLPRFGRKIESI
                     ERTFQFFPEDFDTTVAVAVTHTLKGYLNIRDSNEVQVSKPLEHSLNRVITFAKRDNKW
                     VHTSISGAFGYSENSKLEMDSLIIRTANDTIVIGNATDFIFYTRRPFSVKRGDSISVT
                     VRAFNKSNLLDRPMGSVLNGQNKTRERTRFNMNAFLGGRYERTITLKPTHKVGFNQLV
                     VDFMTPSSLSININKPYDSFMILIPYFVNE"
     gene            3004..4212
                     /locus_tag="PK937_13475"
     CDS             3004..4212
                     /locus_tag="PK937_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNH33631.1"
                     /translation="MIKKFGRAIQWISWGLLTFSFCQCGNSDETTLSKSYPAITAKFG
                     QQIDVKNLANYEDQAIPTYITKDNTGLNTITNEKATVGRVLFYDKNLSFDNSISCAGC
                     HHQSLAFGDTALASRGVQGGLTGRHSMRLVNSRFASETRFFWDERAISLEDQTTRPIK
                     DHAEMGFSGESGRPGFDSLLIKLSAIDYYRELFDLAYGDETITEARLQECMGQFIRSI
                     QSFDTRYDAGRAQVTSDLDNFPNFTTQENQGKTLFITPPVFNASGERTSGGLGCAGCH
                     RAPEFDIDPNSGNNAIIGKLNADGFDVTNTRAPSLRDMTGPDGEPHTPMMHTGVITTL
                     QAAIGHYGTITIGANNTLLDPRLKPNGFGQKLNLTSAEVNAVIAFLKTLSGTQVYTDA
                     RWSDPFGTQP"
     gene            complement(4245..5969)
                     /locus_tag="PK937_13480"
     CDS             complement(4245..5969)
                     /locus_tag="PK937_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH33632.1"
                     /translation="MNPTNWPVWAQDMALKIQSKTTSQFILYNNVRDLMALENPKFPG
                     PMDYVAVGDFLAGSVFKERNTVLFYDISKGVYFLDSAMQAEFFKTMQITQQDLMKLPS
                     YALKAIEIYIRLSVEKKRSVAVIIDHAETVIPATLTSAMSLEDRKCLVMLRKWANDPS
                     YIKNNISIVLITDTLNDLNNRLVRNTSTDKVEVAYPDESERIHYIRYFIDEEIREKAP
                     RLFADYASVTPEQIAHTMAGLNRRQLKILLAYARENNKRIDFAYLVAMKKEIIEEECF
                     GMLEIIEPRHDLNAVVGYDAIKSKLRNLAATIKMGKFDAVPMGYLVSGPVGTGKTFIV
                     LSFVGEIGIPCVKFLNFREQWQGVTEANLEKILNLLRAMWPIGVIIDEADAFLGDRDQ
                     QGDSGTSNRIFAQLASFMGDTSYRGKVIWFLITCRPDLLPIDMKRQGRAEEHISVFYP
                     ETSEEKELLFRSMTRRLNAKIDESIHLKDVFDIHQTVSGADMEALVTRAKLQQAIVGA
                     DTVTVEMLRSIFQDFIPTNDPESVELQNLAAVIECTSKSLLPQKYATMDRSEIRRKFD
                     ELLKRYNQ"
     gene            complement(5983..>8959)
                     /locus_tag="PK937_13485"
     CDS             complement(5983..>8959)
                     /locus_tag="PK937_13485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNH33633.1"
                     /translation="EREVSRWNYAEALRQYENVLPLIVDAEKQTEISFAMELLADHLS
                     DRKLQNALLLHIEKQIHSPHPRFVSFIIRRALYLERTGQPDEGVLFCQKHLESDVIKA
                     KQDRARIRRQIGKYKYQKTLFTEARSDFEEAAHMAGETDDITLQMECQNSLGTVYGSL
                     GEYALADKAFREVLRLARKTEAMASEVSALFNLGQIAFRCGALLQALTWYDEAANLLS
                     LHPNNSQLLRLYLYRASARIRLRQFEMAFEELQSATAKAMTLNDRTALQRILQSKGVI
                     YQNVGLYELAQSELLQAIEVGQNTNPLNTHIVKLEYSHVLFELGDRVEAHRYCKEAIL
                     FFEEKKEDVWLCYAQSVNQILHEYDYSGDETWLQLDALSSKIQDYSVRLFAQQVIAQS
                     LVKKGNYDEALIRLQKVVAEIENQHQYDYSESPIHWLHYRILRTLHKPRYELAKAMDN
                     AYRTMQADLDKLHNPAWRTAYENISLHSTIRAAYEDFFKEERETDIRSFQQLYEITRD
                     INSILDPEKLFEKIMDGALFNARADRGLILLRNEETGGFDVRVARNVDSDSMADLTTI
                     SQSIVDEVYASGASMVTSDANSDMRFKERKSIVAYQIRSILCVPLKLRDHIIGAVYVD
                     KRFDTHYFNSADLKFMEAFAHLAAIAISNAQDYEKVQSENDSLIHENLDLRKAVEERF
                     TRFNIIGKSKAVDQIYRMIEHAAKTDAPVLITGESGTGKELVARAVHHQSLRRRNKFV
                     AVDCGAVTESLLESELFGYKKGAFTGAFTDKKGLFEEADGGTIFLDEITNTTLSFQSR
                     LLRVLQEQEIRRVGDTIPRKIDVRVVAATNKDVRQLITDGQFREDLFFRISVIPVFLA
                     PLRERREDIPLLIKYFVEKYTQPGRMMEGLFGDALQILTDHDWPGNIRELENTVQRLM
                     IFSGKSKLGPSDVRKAMQPSAAPLKSLEDFEKNLFESERKYFERVLDHVGGNKSKAAE
                     WLGIKRTTLNDRLKKLGIS"
BASE COUNT         2384 a         2151 c         1913 g         2511 t
ORIGIN      
        1 atgcggttat cggtattgcc gactccttcc gaacaagggc gaccgctcac ggctaaaaaa
       61 aagacggcca aataaagtgc cgtgatttcc ttcatgattc ggtgcacttg tttttgtcga
      121 gatataaaaa aattgttcat cgtagtaaca aaaaaagtcc cgttagtgca acgggactta
      181 tagtacaaac gatcagctaa aaaatcaaaa aactagcggc cttttttctt atggcggtcg
      241 cgacgaagac gttttttacg tttgtgagta gccatttttc ttcttttgcg tttttttccg
      301 cagggcatgg ttagttcctt tctttttttt gtgtgttata tgttatgatc ggatttcgtt
      361 gaccgatttt ttcaattctt tcgatggcct aaattgcggt acacggcgct ccggtaaatc
      421 taccgccgca ccggttttag ggttgcgtgc cgtacgcgcc gggcgtcgag caatacgaaa
      481 gctgccaaac ccgcggattt cgatttcacg gccttgctgc agttcttcat ggatcacggc
      541 gataaacgca ttgataaccg catcggtatc aaatcgcgta atgcccgtgg cctccgcgat
      601 gcgatggatg atatccgctt tcgtcgtatt ggttgccata tactcagatg ccgtatttat
      661 aactcaccac cgggtaactt ttgaaacccg gaatactcag cgccgattcg attttactct
      721 ctgcatcttc ttcaaacaac agatcaaaaa acgaaggctt gtgttttttc tctttgacga
      781 ggttcggcgt accttcgatg cctgccatgt ccgccgccag acggatcgca tcgtcgtacg
      841 aaccgatcac atcaatcagc ttgatttgca acgcctgctc acccgtatac acacgtccgt
      901 ccgcatattg ttgtacatac gccgtatcca agccgcgttc atccgcgacg gcacgtacaa
      961 attgtccgta cgcattcatc acaaacgatt gcatccactc ggattccgac ggattcatcg
     1021 ggcgatacgg gctcaaaata tctttgaacg caccgctttt gatcgtcgta aaatccacac
     1081 cgattttatc caaaagttta tccacattgg ggaactgaat gatcacgccg atactgcccg
     1141 tgatcgtgcc gggattagcg acgatcgtat ccgccggaca cgcgacataa tatccgccgg
     1201 acgctgctac gctacccatc gaaacgatga ttttttttcc ggccgctttt gtttttttga
     1261 cgatatcaaa aatttcctgc gaaggcgtga taccgccgcc ggggctctca atgcgaaata
     1321 caatcgcttc gacggagctg tcatcacggt attttttgaa ctggcgcact atatcgcgcg
     1381 aatcaacgat cgtaccttcg agttcgatca atgcgatgcg ccgaccgaaa tttacatccg
     1441 gctccgcaac gcgatatacg acgtaaatga tcagcccgac aaaacacgac aaaaaaccca
     1501 aaagcaaaat ccatgtcgaa cgatttttca tacttctatt tttgtatact taatttttta
     1561 attcaatgtt acttaaccga actataccca ttacagcgtg taaatatatc attttcaagg
     1621 ctactgtcaa ttgatattgt aaatactttc aaagacatgc ttttcatgaa aaatttgaaa
     1681 aaaacatata atcaatctgc ttatatatgt agcaattgca tatgcttgcg ttttaagtta
     1741 aactacatag acggttttcc cgtcaaaaat ccgttgattc gataattaaa gtaatgtaaa
     1801 acaaataatt aaatgcggca tgtattttga tatatattcg ggccggaggt gttcttatga
     1861 aacaagaaga ttattctact accaatttaa atttacgcag tgagattctg caaatctcgg
     1921 gattatccgt actgatactg cttttgctgt tgtttttagg cggatgcagc attagtggag
     1981 acgattttac agataccgtc caggatgaaa tcaaacaaat catcaaagag gatgaacaat
     2041 tggcttacgg cgtagaggtg gtcaacgatt cctcggaaat caccgcgcca caaaagagcg
     2101 ccgcttcgga gagcgctttg gtggaattgc ttccgcgttt cggacgtaag atcgaaagta
     2161 tcgaacgtac attccaattt ttccctgaag attttgacac caccgtcgca gtagcggtta
     2221 cccatacgtt gaaaggttat ctgaatatcc gggattccaa tgaggtacag gtcagtaagc
     2281 ctctcgaaca ttctctgaac cgtgttatta cttttgccaa acgcgataac aaatgggtgc
     2341 acacgagcat ttccggcgcc ttcggatatt cagagaattc aaaattagaa atggattcgc
     2401 ttattattag aacggctaat gacaccatcg tgatcggcaa tgccaccgat tttatatttt
     2461 atacacgtcg tccgtttagc gtcaaacgcg gcgactcgat cagcgttacc gtgcgcgcat
     2521 tcaataaatc taatttactg gatcgtccga tgggttcggt gcttaacggg caaaataaaa
     2581 cccgtgaacg tacacgtttt aatatgaatg cgttcttagg aggtcgttac gagcgaacca
     2641 taactctcaa accaacacat aaagtcggtt ttaaccagct tgtagttgac tttatgacac
     2701 cgtcttcgct cagtatcaac atcaataagc catacgattc gtttatgatc ttgatcccat
     2761 attttgttaa tgaataaaat cgcaaacgaa catataagcg cctcatattt gtatagacat
     2821 ccgcttgcgc gggtgaggca gttctgtcgt tcccgcgaaa gcgggaaccc agtgagaaac
     2881 gagaggttgt gttcgccatt atgaagttga ttgaatgaca atgttgttac aattatgaga
     2941 ccgttaatat ctttgattca cagtgcactt ttaaaaaacg cagaaagctg caacaaaatt
     3001 attatgatta aaaaatttgg tcgcgccatt caatggatca gttggggact tttgacattt
     3061 agtttttgcc aatgcggtaa ttctgatgag acgacattat ctaaatctta cccggccatt
     3121 acggcgaaat tcggtcagca aattgatgta aagaatctgg ccaattatga agaccaagcg
     3181 attcctacct atattaccaa agacaacacc ggccttaata caattaccaa cgaaaaggca
     3241 acggtgggaa gggtactttt ttacgataaa aatctcagtt ttgataattc gatttcctgc
     3301 gccggctgcc atcatcagtc gctcgctttc ggtgatacgg cgctggccag tcgcggtgtt
     3361 cagggcggat tgacaggccg gcattccatg cgtttggtca attcacgttt cgcatccgaa
     3421 acccgttttt tttgggatga acgtgcgatc agccttgaag atcaaacaac acgaccgatc
     3481 aaagatcatg cagagatggg cttcagcggt gaaagcggtc gaccaggctt tgattcgtta
     3541 ttaattaaat tatcggcgat agattattat cgtgaattgt ttgatttggc ctatggtgac
     3601 gaaactataa cggaagcacg tttgcaggaa tgtatggggc aatttattcg cagcatccaa
     3661 tcttttgata cgcgttacga cgcagggcgc gcacaggtga cttccgatct ggataatttt
     3721 cctaatttta caacgcagga aaaccagggc aaaacgcttt ttatcacacc acctgttttt
     3781 aatgcctccg gggagcgcac aagcggcggg ctcggctgtg cggggtgtca cagagcgccg
     3841 gaatttgata ttgatcccaa tagcggaaac aatgccatta tcggaaagct caatgccgac
     3901 ggttttgatg tgaccaatac acgcgcaccc tcgctgcgtg atatgaccgg gccggacggc
     3961 gaaccccata caccgatgat gcacaccggt gtgatcacga cactacaagc ggctatcggg
     4021 cattatggaa cgatcaccat tggcgctaat aatacactcc ttgatcctcg attaaaaccc
     4081 aacggatttg gccagaaact caacctgacc tccgctgaag ttaacgccgt catcgctttt
     4141 ctgaaaacgt tatccggtac acaggtatat accgatgccc gttggagcga tcctttcgga
     4201 acgcagccgt aatgtttttt agataagatc ggaaaaatga aaatttattg attgtaccgt
     4261 ttgagcagtt cgtcaaattt tctgcggatt tcgctgcgat ccatcgttgc gtatttctgc
     4321 ggcagcaggc ttttgctggt acactcaatc acggcggcta agttttgcaa ttcaacactt
     4381 tccggatcgt tcgtgggaat aaaatcctga aaaatcgaac gcagcatttc taccgtaacc
     4441 gtgtcggcac cgactatggc ttgctgaagt ttggcgcgtg tcaccagcgc ttccatatcg
     4501 gcaccggata ccgtctgatg gatatcaaac acatctttca gatgtatgct ttcatcgatt
     4561 tttgcattca gccgccgggt catcgaacgg aacaataatt ctttttcctc cgatgtttcc
     4621 ggataaaaca cgctgatatg ttcttccgca cgaccttgcc gcttcatatc gatcggcaaa
     4681 agatcagggc ggcatgtgat caaaaaccag atgactttgc cgcggtacga cgtatcaccc
     4741 ataaagctgg ctaattgcgc aaaaatccga ttgctcgtac cgctatcgcc ctgctggtcc
     4801 cgatctccga gaaacgcatc cgcttcatca atgatcacac cgatcggcca catagcccga
     4861 agcagattga gtattttttc taagttcgct tccgtcaccc cctgccactg ctctcgaaaa
     4921 ttgagaaatt tgacacaagg aatgccaatc tcaccgacaa aggataacac gataaatgtt
     4981 tttcccgtac ccaccggacc acttaccaga taacccatcg gtacggcatc aaatttaccc
     5041 attttgatag tggccgccag attacgaagt ttacttttga tcgcgtcata acctacgacc
     5101 gcgttcagat catggcgcgg ctcgatgatc tcgagcatgc cgaaacattc ttcttcgatg
     5161 atttcctttt tcatcgccac gagataggcg aaatcaatac gtttgttatt ttcccgagcg
     5221 taagccaata aaattttgag ttgccgacgg ttaagaccgg ccatcgtatg cgcgatttgc
     5281 tccggtgtaa cggacgcata gtcggcaaaa agcctcggcg ctttctcgcg tatctcttca
     5341 tcaataaaat aacggatata atgaatacgc tcggactcat cggggtatgc cacttctact
     5401 ttatccgtgg acgtattgcg tacaaggcga ttattgagat cgttgagcgt atcggtaatt
     5461 aatacgatcg aaatattatt tttgatgtaa ctgggatcgt tagcccattt gcgtagcatc
     5521 actaaacatt ttcgatcttc gagtgacatg gccgatgtca gcgtcgctgg gatcaccgtt
     5581 tccgcgtgat caatgatcac ggctaccgaa cgtttttttt ccacactgag acggatatat
     5641 atttcgatgg ccttgagtgc ataactcggc agtttcatca gatcctgttg cgtgatctgc
     5701 atggttttaa aaaattcggc ctgcatcgca ctgtcgagaa agtacacccc ttttgaaata
     5761 tcgtaaaaca aaaccgtgtt gcgttcttta aacaccgacc cggctaaaaa atcaccgaca
     5821 gcgacgtaat ccattgggcc cggaaatttt ggattttcta aggccatcag atcccgcaca
     5881 ttattgtaca aaataaattg cgatgtcgtt ttcgattgaa tttttagggc catatcttgc
     5941 gcccataccg gccagttggt tggattcatg tatcctcttg ttttatgaaa tgcctaattt
     6001 tttcaaccga tcattgagtg tcgttcgttt tataccaagc cattcggcgg ctttggattt
     6061 gttgccgccg acatgatcca aaacgcgttc gaaatatttt cgttcggatt cgaataagtt
     6121 tttttcaaaa tcttccaatg atttcaacgg cgcggctgaa ggttgcattg cttttcgaac
     6181 atccgaaggg ccgagtttgg atttacccga aaaaatcatc aaccgctgta cggtattttc
     6241 caattcgcga atattgcccg gccaatcatg atccgtcaaa atctgaagtg catcaccgaa
     6301 aagaccttcc atcatacgcc ccggctgcgt gtatttctcg acaaaatact taatcagaag
     6361 cggaatgtct tcgcgacgct cacgcagggg cgccaaaaat accggaatga cagaaattcg
     6421 gaaaaacaaa tcctcccgaa attgaccgtc cgtgatcagt tgacgtacat ctttgtttgt
     6481 agcagcaacg acgcgtacgt ctattttacg agggatcgta tcgcccacgc ggcgaatttc
     6541 ctgctcctgt aaaacgcgca gtaaccggct ttgaaacgac aaggttgtat tagtaatttc
     6601 atcgagaaaa atagtcccgc cgtcggcttc ttcaaacaat ccttttttgt cggtaaacgc
     6661 gccggtaaac gcgccttttt tataaccaaa aagctcgctt tccaaaagcg attccgtcac
     6721 agcaccgcaa tctacggcca caaacttatt acgacgacgc agactttgat gatgaacggc
     6781 ccgggcaact aattctttgc ccgtaccgct ttcgcccgtg atcagtaccg gtgcatccgt
     6841 tttagctgca tgctcgatca tacggtatat ctgatccaca gctttgcttt ttccgataat
     6901 attaaaccgc gtaaatcgtt cttcgaccgc tttgcgtaaa tccagatttt cgtgtatcag
     6961 cgagtcgttt tcggactgca ctttttcata atcttgtgcg ttggagattg cgatcgccgc
     7021 taaatgggca aacgcttcca taaacttgag gtcggcggaa ttgaagtaat gcgtgtcaaa
     7081 acgtttatcc acatacaccg caccgataat atggtcgcgc aacttgagcg gtacgcataa
     7141 gatggagcga atctgatagg ctacaatact ttttcgttct ttgaaccgca tatcggaatt
     7201 cgcatccgac gtgaccatgg atgcgccgga ggcatatact tcgtccacga tactttgact
     7261 gatcgtcgtc agatcggcca tcgaatccga atccacattg cgggcgacac gcacgtcaaa
     7321 cccgccggtt tcttcgttac gcaataaaat caatccgcga tccgcgcggg cgttgaacaa
     7381 tgcgccgtcc atgatttttt caaagagttt ctccggatcc aagatgctgt tgatatcgcg
     7441 cgtgatttca taaagctgtt gaaacgaccg aatatccgtc tcgcgttctt ctttaaaaaa
     7501 atcttcgtac gcggctcgga tggtcgaatg cagcgatata ttctcatacg ccgtgcgcca
     7561 tgcgggatta tgcagcttgt ccaagtccgc ctgcatcgtc cgataagcat tgtccatggc
     7621 cttagccaat tcataccggg gtttgtgcaa cgttctgaga attcgataat gcagccaatg
     7681 aattggcgat tcggaatagt cgtattgatg ttgattctca atctctgcga cgactttttg
     7741 aagacgaatg agcgcctcgt cgtaattgcc tttcttaacc aaactttgtg caataacttg
     7801 ctgggcaaat aatcgaacgg aataatcctg aattttggaa gacaatgcat ccagttgcaa
     7861 ccatgtttca tcaccggaat aatcgtattc atggagaatc tggtttacac tttgcgcata
     7921 acataaccac acgtcttcct tcttttcttc aaaaaacaat atcgcctctt tgcaataacg
     7981 atgcgcttcc acgcgatctc caagctcaaa cagtacatga ctgtattcaa gtttgacaat
     8041 atgcgtgtta agcgggttcg tattttggcc tacctcaatc gcctgaagta attcgctttg
     8101 agcgagttcg tacaaaccta cattctgata aatgacacct ttagactgca ggatacgttg
     8161 caaagcggtg cgatcattta aagtcatcgc ttttgccgta gcgctttgta attcttcaaa
     8221 agccatttca aattgccgca aacgtattcg ggcagaagcg cgataaagat atagacggag
     8281 cagctgactg ttattcggat ggagcgataa aaggttagcc gcttcatcgt accatgtcaa
     8341 agcttgcaac agcgcaccgc accgaaacgc tatttgaccc agattaaata gggccgatac
     8401 ttcggaagcc atcgcttccg ttttacgcgc cagacgcaat acttcacgaa acgctttatc
     8461 tgcaagcgca tattcgccga gcgaaccata taccgtacca aggctgttct gacattccat
     8521 ctgcagtgtt atatcatctg tttcaccggc catgtgtgcc gcttcttcaa aatccgaacg
     8581 cgcctccgta aataatgttt tctgatactt gtatttaccg atttgccgac ggattctcgc
     8641 tctgtcctgt ttggccttga taacgtcgga ttccaaatgt ttttgacaaa acaacacgcc
     8701 ttcgtccggt tgacctgttc gctccaaata caacgcacga cgtatgataa atgacacaaa
     8761 acgcggatga ggtgagtgaa tttgtttttc tatgtgtaac aataatgcgt tttgtaattt
     8821 acggtcagac aaatgatccg ccagtaactc catcgcaaac gatatttcag tttgtttttc
     8881 cgcatcgacg atcaatggca aaacattttc gtattgacgc aaagcttccg cataattcca
     8941 acgcgacact tcacgttcg
//
LOCUS       DAOQGF010000126         8612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3888_length_8612_cov_6.931401, whole genome shotgun sequence.
ACCESSION   DAOQGF010000126 DAOQGF010000000
VERSION     DAOQGF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_8612_cov_6.931401"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK937_13490"
     CDS             complement(<1..454)
                     /locus_tag="PK937_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH33634.1"
                     /translation="MAVPLLDLKAQYRAHKSEIDEAMLRVAESQHFIMGPEVEKLEKS
                     LCTYLECKHAIAVSSGTDALLVALMALDIQPGDEVIVPTYSFFATAGVVARLHAKPVF
                     VDSDPLTFNIRASDIEKKITAKTRVIMPVHLFGQSSDMGAIMAELCPKR"
     gene            complement(454..1041)
                     /locus_tag="PK937_13495"
     CDS             complement(454..1041)
                     /locus_tag="PK937_13495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNH33635.1"
                     /translation="MSEKKYFVHPLAVVDDNVEIGEGTKVWHFSHIQTGARIGKKCVF
                     GQNVNVANHVKVGNFCKVQNNVSIYEGVELEDYVFCGPSMVFTNILDPRCKYPQVGAE
                     FYIKTLVKEGASLGANSTIVCGHTVGRSALVGAGAVVTKDVPDFALVVGNPARIVGWV
                     SEAGKKLNFDAQGFAYCEKSKKKYKFENGLVKEIE"
     gene            complement(1034..2017)
                     /locus_tag="PK937_13500"
     CDS             complement(1034..2017)
                     /locus_tag="PK937_13500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH33636.1"
                     /translation="MDYTKVKLGIIGCGNWGLNHVRTAHGLLGSNLLLTADAKPDAIQ
                     RVHTVSPKIPFTSDIHSLIAHSNINAVVIATPAETHFDLAKRCIENGKHVLIEKPITL
                     ISEHTVELLSLAQKYDVKIMVGHVLLYHPAVLKLKQMMDEGYIGKLQYIYSNRLNLGA
                     IRSEENSLWSFAPHDISIVQYLIGKNPETIEAKGAAFVQKNVEDSTITYLTYPGNIHV
                     HVFVSWLHPFKEQRLVVIGDKGMIVFEDSLKTDKLKFYKKGFQQSNGKIEKFEVDFEV
                     IPFDAAQPLSEEHRHFYDCLLHGKTPRTDGEHALEVLRILEKAQERITQHV"
     gene            complement(2021..3661)
                     /locus_tag="PK937_13505"
     CDS             complement(2021..3661)
                     /locus_tag="PK937_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNH33637.1"
                     /translation="MKKHLFLTMISALILSAMTDTTVLAQQDRSKIKEELEKDNSSGD
                     PDDVLFPEKSNNILETVVDPKQYYVGPGDQFLVSIWGQVNQNFRTVVSPEGFLIVPTV
                     KAIDVKNLNLADVQERVGKSIRTVYTNAEVTINLVSLRSFRVPVGGMCNNPSSVVVSG
                     TERVSTAIAKAGGLNGKASQRNIILYRKDGTSQSVDFIKRGRNLDLDSDPLLNDGDRI
                     FVPAQYASYQILGAVASHGTMEYVANENIIDAIRLAGGLVQGIDSSIFIMYRMAKDTG
                     REVIRITRDLREAYQNPEDTTINLRLKPDDRIYFRKHAQYHDRADVAIQGEVMYPGSY
                     AIIEDKTTVSDVIKRAGGFTPYVSLDRISIYRSPAKEDIDQEYERLKLTPVSEMNDIE
                     KSYFKTKTRQQKPVVQTDFKKLFADGKVNSEYDVVLKRGDYIDVSSIRRTVTVVGGAL
                     LSGIVDFDHGKDYRYYIDKCGGFTDRAKKGDIKVIKTASQKWLDADDDVLIEDGDVIF
                     VPEKEPVDGWQIFKDALTITGQLAAITSTIILIYFTVK"
     gene            complement(3658..4749)
                     /gene="wecB"
                     /locus_tag="PK937_13510"
     CDS             complement(3658..4749)
                     /gene="wecB"
                     /locus_tag="PK937_13510"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNH33638.1"
                     /translation="MKLLCIAGARPNFMKIAPILRAIKNYPQITSKLIHTGQHYDAVM
                     SDIFFKELQMPDPDIHMGVGGGSHAQQTADLMLKFDSILVNEKPDYVLVVGDVNSTMA
                     CAVTASKLHVPVIHVEAGLRSFDRTMPEEINRLVTDAISDYLFTTESSANQNLKNEGK
                     QENQIFFAGNVMIDSLTFALPKIEASDILQRMNLKEKEFVLVTLHRPANVDSEENLRS
                     MLGLLEFIAGYMPVVFPVHPRTKTKLTQYGMDHKVKLNERIRLIEPLGYFDFIALMRS
                     SVAAVTDSGGVQEETTFLGVPCLTMRANTERPVTLDVGTNTLVGQDRDALKSCMEAVL
                     NRSYKKGNIPALWDGMAAERIAEKLKDLV"
     gene            complement(4757..6079)
                     /locus_tag="PK937_13515"
     CDS             complement(4757..6079)
                     /locus_tag="PK937_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNH33639.1"
                     /translation="MNLESKIKNKSAKVGIIGLGYVGLPLAVEFAASGYDTLGIDLDS
                     KKVKSINDGHNYIEDIPNDRFQKCYQSGKLKAETTYQRIGEMDIIYICVPTPFTDNKE
                     PDVSYIVSASEGIASGLRKEQLVILKSTTFPETTEKVTMPILDKKGLKVGADYYLAFS
                     PERIDPGNKKYTTANTPVVVGGVTSKCTELACLINAQIIEKVVPVSNPRVAEMCKLLE
                     NIFRSVNIALVNELAQLCDRMGGVDMWEVINAAGTKPFGYMPFYPGPGIGGHCILIDP
                     YYLSWKARGYDFHTNFIELAAEVNENMPYYAYSMIVEALANRGVALKKAKVLVLGAAF
                     KKDVDDTRHSPSLKVMEIMIQKGFSDVMYNDPYVPEVELLGKVYKSQAINTELLKSVD
                     VVFVGTDHSRYDYNEIVSHAASVVDSRNATKSVTHGRDKIAVLGSGKV"
     gene            complement(6112..7425)
                     /locus_tag="PK937_13520"
     CDS             complement(6112..7425)
                     /locus_tag="PK937_13520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNH33640.1"
                     /translation="MKKICVVGTGYVGLVSGACLADFGNNVICADIDKEKIDKLNQGV
                     IPIYEPGLKEVVDRNVEKKRLFFSNDVAQSIKESEVIFIAVGTPPGPKGDADLSIVFK
                     VAETIAKNLNGYKVVVTKSTVPAGTGEKVRKILSEHAPKGAEFDVVSNPEFLREGSAV
                     SDFLRPDRVVIGSNSDRATEVMKTVYRALYINETPMVVTNVESSEMIKYASNAFLAVK
                     ISFINEIANICEKIGADVKVVAKAMGLDGRISPKFLHAGGGFGGSCFPKDTMALAKTA
                     EEVGVQNLVVNAAIQANDYQKMLMVEKIRKMVGGNFKGKTIAVLGIAFKPNTDDIREA
                     TSLVIMEEITKEGGVVKAYDPVAMEQAQKFFPTMHYAKDAFEAVQGADCMVIVTEWNE
                     FRELDLVKAKTLLKTAKIVDCRNIFDPKEIKALGFEYDAVGRITK"
     gene            complement(7441..8259)
                     /gene="rnc"
                     /locus_tag="PK937_13525"
     CDS             complement(7441..8259)
                     /gene="rnc"
                     /locus_tag="PK937_13525"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNH33641.1"
                     /translation="MSPGTSQEHAVHGGWFNGIIRFFRRAWGPEQADGFLPDNGLRLQ
                     DLSRVLGVRIENPKLYIQALKHRSFLNVSNEKHFSSYERMEFLGDAILSTITAEFLFQ
                     KYPESDEGFLTKGKSHLVNKKTLAHKAEALGLGQFIVMSDGEERSGGRSRSSILSDVF
                     EALIAAVYLDHGYHTARKFVLDTLLCDTERILQSEMNSNFKGDLLEYTQGKDLGIPQY
                     IVVSETGPEHQKEFTIQVAVKDTILGCGRGLSKKEAEQQAAKEALQRIKNNGIQ"
     gene            complement(8265..>8612)
                     /locus_tag="PK937_13530"
     CDS             complement(8265..>8612)
                     /locus_tag="PK937_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HNH33642.1"
                     /translation="YINAHGTSTEYNDKNESAAIKTVFGDHAYKLLVSSTKSMTGHLL
                     GAAGGIEFIATTLAVKNGWIPPTINYEVSDPACDLNYVPNKAIQRNVDVALSNTFGFG
                     GHNTCIAVTKYKP"
BASE COUNT         2354 a         2111 c         1671 g         2476 t
ORIGIN      
        1 accttttcgg acaaagctcc gccatgatcg ctcccatgtc ggagctttgt ccgaaaaggt
       61 gcaccggcat gataacgcgt gttttggcag tgatcttttt ttcaatgtca gatgcacgga
      121 tattaaaagt cagcggatcc gaatcaacaa aaaccggttt tgcatgcagt cgggcaacca
      181 cgccggcagt ggcaaaaaaa gaatacgtcg gcacgattac ttcatcgccg ggctgaatat
      241 cgagcgccat cagcgctacc aataacgcat ccgtcccgga agaaactgca atcgcatgtt
      301 tgcattccag ataagtacac aaagatttct ctaatttttc aacttccggg cccataataa
      361 aatgctgcga ttcggcaacg cgcaacatgg cttcatcaat ctccgatttg tgagcacgat
      421 attgtgcttt gaggtctaaa agaggtacag ccattattct atttccttta ccaaaccgtt
      481 ttcaaattta tattttttct tggatttttc gcagtaagca aaaccctgtg catcaaaatt
      541 taatttctta ccggcttcgc tgacccaacc gacgatgcgt gccggatttc cgacgaccaa
      601 tgcaaaatcg ggaacgtctt tagtaaccac cgcaccggct ccgacaagag cgctgcgtcc
      661 gacggtatga ccacatacga tggttgagtt tgcgcctaac gaagcacctt cttttactaa
      721 ggttttgatg taaaactctg caccgacttg cggatacttg cagcgcggat caagaatatt
      781 tgtaaaaacc atcgaaggtc cgcagaacac ataatcttca agctccaccc cttcgtagat
      841 ggacacatta ttttgtactt tgcaaaaatt accgactttt acatgattgg ccacatttac
      901 attttgacca aaaacacatt tttttccgat acgagcgccg gtttggatat gcgaaaaatg
      961 ccaaacttta gtaccttcgc cgatttcgac gttgtcatcc acgacagcca agggatgaac
     1021 aaaatatttt ttttcagaca tgttgcgtga tcctttcttg tgctttttcc aatatccgaa
     1081 gcacttccag cgcatgttcg ccgtccgtac gcggtgtttt gccgtgcagg aggcaatcat
     1141 aaaaatgacg atgttcttca cttaatggtt gtgcggcatc aaacggaatc acctcaaagt
     1201 ctacctcaaa tttctcgatt tttccgttac tttgctgaaa gcctttttta taaaatttta
     1261 acttatctgt tttgagcgaa tcttcaaaca cgatcattcc cttatcaccg atgacaacca
     1321 gtctttgttc tttaaaaggg tgaagccaac tcacaaaaac atgcacatga atgttaccgg
     1381 gataggtgag gtacgtgatg gtcgaatctt cgacgttttt ttgtacgaac gccgcacctt
     1441 tagcttcgat cgtttccgga ttttttccta tcaaatattg aactatcgaa atatcatgcg
     1501 gtgcaaaact ccaaagacta ttttcttcgc tgcgaatagc gccgagattg agccgattac
     1561 tgtaaatata ctgaagtttg ccgatatacc cttcatccat catctgtttc aacttcaaaa
     1621 ccgccggatg atacaaaagc acatgcccta ccatgatttt tacatcatat ttttgagcta
     1681 aactcaaaag ttcaacagta tgttcagaaa ttagcgtgat tggtttttcg atcagtacat
     1741 gtttgccgtt ttcgatacat cgtttagcaa gatcaaaatg ggtttcggcc ggcgttgcaa
     1801 tcactacggc attgatattc gaatgcgcga tgagcgaatg aatatctgat gtaaatggaa
     1861 ttttcggtga aactgtatgc actctttgga tggcgtcggg tttggcatcc gccgttaata
     1921 aaagattact tcctaataac ccatgggcgg tacgcacatg attgagtccc caatttccac
     1981 aaccgataat acctaatttt acttttgtat aatccataat ttattttaca gtaaaatata
     2041 taagtataat ggttgatgtg atggccgcca gttgacctgt tatagtcagc gcatctttaa
     2101 atatctgcca accatctacc ggttcttttt ccggaacaaa aatcacatcg ccgtcttcga
     2161 tcagtacatc atcatcggca tccagccatt tctgcgaagc cgttttgatg actttaatat
     2221 cacctttttt agctctatcc gtaaaaccac cgcacttgtc aatataatat cggtaatctt
     2281 tgccgtgatc aaaatctact atacctgaca ataacgcgcc gccaacaacg gtcacggttc
     2341 gccggataga tgacacatca atataatcac cgcgctttaa aacaacatca tactccgaat
     2401 tcactttccc gtcagcaaat agttttttaa agtcagtttg tacgacgggt ttttgttgac
     2461 gtgttttggt tttgaagtaa gacttttcaa tgtcgttcat ctcggacacc ggtgtcaatt
     2521 tcaggcgctc atattcttga tcgatatcct cttttgcggg actgcgataa atggaaatgc
     2581 gatccaatga tacatacggc gtaaaaccac cggccctttt aatcacatcc gaaacggtcg
     2641 ttttatcttc gataatggca taggatccag gatacatcac ttcgccttgg atagctacat
     2701 cagcccgatc atgatactgc gcgtgttttc ggaaataaat acgatcatcc ggtttgagac
     2761 gcagattgat cgtggtatcc tccggatttt gatacgcttc acgcaaatcg cgcgtaatac
     2821 gtatcacctc tcggcctgta tccttggcca tacggtacat gatgaaaatg gatgagtcaa
     2881 tcccctgcac taaaccaccg gccaatcgaa tagcatcgat gatattctca ttcgccacgt
     2941 actccatagt accatgactt gctacagcac ccaaaatctg ataacttgca tattgagcag
     3001 gcacaaatat acggtcaccg tcgttaagta atggatccga atccaaatct aaatttcggc
     3061 cgcgttttat aaaatcaaca ctctgcgatg taccgtcctt acgatacaga ataatatttc
     3121 tttgcgatgc ctttccgttt aaaccgcccg ctttcgcaat ggctgtcgat acccgttctg
     3181 ttcctgagac cactaccgaa ctcggattat tacacatacc ccctacgggc acgcgaaaag
     3241 agcgaagtga aaccaggttg atcgtcacct cggcattggt atacacagtt ctgatacttt
     3301 ttcctacacg ctcctgcaca tcggctagat tgagattttt aacatctatg gcttttactg
     3361 taggaacaat caaaaaacct tcgggcgata caacggttct gaagttttga ttgacctgcc
     3421 cccaaatcga aactaaaaac tgatccccgg gaccaacata gtattgtttt ggatctacaa
     3481 ccgtctcaag aatattattg gatttttcag ggaataaaac atcgtccggg tcaccggaag
     3541 agttatcctt ttctaattcc tctttgattt tagatcgatc ctgttgcgcc aatactgtgg
     3601 tatccgtcat cgccgaaaga atcagagctg aaatcattgt taagaataaa tgctttttca
     3661 tacaagatcc ttcagttttt cggcaatacg ctccgccgcc ataccatccc atagagccgg
     3721 aatatttcct tttttgtagc tacggttcaa tacggcttcc atacacgatt tcaacgcatc
     3781 ccgatcttgt cctaccaacg tattagtacc gacatccaaa gtaaccggcc tttcggtatt
     3841 ggcgcgcatg gtaagacatg gtacacccaa aaacgttgtt tcttcttgca caccgccgga
     3901 atcggtaacg gcggcaacag aggaacgcat caatgcaata aaatcaaaat acccgagcgg
     3961 ctcaatcaat cgaattcgtt cgttcaactt gactttatga tccatcccat attgcgtaag
     4021 tttggttttg gtccgtggat gaacggggaa aacaaccggc atataacccg ctataaactc
     4081 caatagacca agcatagacc gtaaattttc ctcggaatcc acgttcgccg gacgatgcag
     4141 tgtgacaagg acaaattctt tttcctttag attcatgcgc tggagaatat ccgacgcctc
     4201 aatcttgggt aatgcaaatg taagcgaatc aatcatcaca ttgccggcga agaaaatctg
     4261 attttcttgt ttaccctcat tcttgagatt ttgattagcc gatgactcgg tagtgaacag
     4321 ataatccgaa atggcatcgg ttaccaaacg attaatttcc tccggcatcg tacgatcaaa
     4381 acttcgtaat ccggcttcaa catgaataac aggcacatga agtttggaag ctgttacagc
     4441 acacgccatc gtcgaattca catctcccac caccaacaca taatcgggtt tttcgtttac
     4501 caaaatggaa tcaaacttca gcatcagatc cgcagtctgt tgcgcatgcg atccaccacc
     4561 aactcccata tgaatatctg gatcgggcat ttgcaattct ttaaaaaaaa tatccgacat
     4621 gaccgcatca taatgctgac ccgtatgaat aagctttgat gtgatctgcg gataattctt
     4681 aatcgctctt agaatgggag caattttcat aaaattagga cgcgcaccgg cgatacagag
     4741 cagtttcatc gttgatttat accttaccgg agccgagtac agctatttta tcgcgcccat
     4801 gcgtcacgga tttggttgca ttacgggaat ccacaacact ggcggcgtgg gagactattt
     4861 cattataatc atagcgcgag tgatccgttc cgacaaacac aacatcaacg gacttcaaaa
     4921 gttccgtgtt gatagcttgt gatttataaa ctttacccaa aagttcaact tcaggaacat
     4981 acggatcatt gtacatcaca tcgctaaacc ctttctgaat catgatttcc atcactttca
     5041 gcgatggtga atgccgtgta tcatccacat cctttttgaa ggccgcaccg agaaccaata
     5101 ctttagcttt tttaagcgca acaccgcgat tggccaaagc ttctacgatc atcgaatagg
     5161 cataataggg catattctca ttgacctccg cagccaattc gataaagttg gtatgaaaat
     5221 cgtagccgcg tgctttccat gacaaataat agggatcaat aagaatgcaa tgtcctccga
     5281 taccagggcc cgggtaaaac ggcatataac caaaaggttt agtaccggcc gcatttatta
     5341 cttcccacat gtccacaccg cccatgcgat cgcagagttg tgccaattca ttgacaaggg
     5401 caatatttac actgcgaaaa atattttcga gcagcttgca catttcggca acccgaggat
     5461 tggatacggg tactactttt tctatgatct gagcattgat gagacaagcc agttcggtac
     5521 acttcgaagt cacacccccg accacgaccg gggtattggc tgttgtatat tttttattgc
     5581 cgggatcaat gcgttccggc gaaaatgcga gataataatc ggcaccgact ttcagacctt
     5641 ttttatcaag aatcggcatc gttacttttt ccgtggtctc cggaaacgta gtgcttttaa
     5701 gaataacaag ctgctcttta cgaagcccgc ttgcaatacc ttcgctggca ctgacgatgt
     5761 acgaaacatc aggttcctta ttatccgtaa agggcgtcgg tacgcatata tagatgatgt
     5821 ccatttcgcc aatacgctga taagtggtct cggctttcaa cttacccgat tgatagcatt
     5881 tttggaaacg atcattgggt atatcttcga tatagttatg gccatcattg atacttttta
     5941 cttttttaga atcaagatca atgcccaggg tgtcgtaacc ggaggcagcg aattcaacag
     6001 ccaaaggcaa ccccacataa cctaatccga tgatacccac tttggccgat ttatttttta
     6061 ttttactctc tagattcacg tggacactcc aacaaatgtt gttttaataa cttattttgt
     6121 gatacgcccc accgcatcgt actcaaatcc gagcgctttt atttctttag gatcgaatat
     6181 atttctgcaa tcaacaatct tagccgtttt tagcagtgtt ttcgccttga ccaaatccaa
     6241 ctcgcgaaac tcattccact ccgtcacgat caccatacag tcagcacctt gcacggcttc
     6301 aaaagcatct ttagcataat gcatcgtcgg gaaaaatttc tgcgcttgtt ccatagctac
     6361 gggatcataa gctttcacaa cgccgccttc tttggtgatt tcttccatga tcacaagcga
     6421 tgtagcttca cggatgtcgt cggtattagg tttgaacgca atacccagca ccgcgatggt
     6481 tttaccttta aaattaccgc cgaccatttt tctgattttt tcaaccatga gcattttttg
     6541 gtaatcattc gcctgaatgg cggcgttgac aacaaggttt tgtacaccaa cttcttccgc
     6601 tgttttggct aacgccatgg tatctttagg aaaacaagaa ccgccgaaac cgccaccggc
     6661 atggagaaat ttcggactga tacggccatc caatcccatc gctttggcaa caacctttac
     6721 atcagcaccg atcttttcgc aaatattggc gatttcatta ataaacgaaa tttttacggc
     6781 cagaaatgca ttcgatgcat atttgatcat ttcggatgat tcgacattgg tcacgaccat
     6841 cggcgtctca ttgatataaa gcgcccgata cactgttttc atcacctctg tcgcacgatc
     6901 gctgttgctc cctatgacca cacgatccgg tcttaagaaa tcactgaccg cagaaccttc
     6961 gcgtaaaaat tcgggattgg ataccacgtc aaattcggca cctttggggg catgctcgga
     7021 aagaatcttc ctgacttttt cacccgtacc cgcaggaacc gtactcttgg tgactacaac
     7081 tttatagccg tttaagtttt ttgcaatggt ttcggcgact ttgaaaacaa tactcaaatc
     7141 ggcgtccccc ttaggtccgg gcggcgtacc taccgcgata aaaatgactt cggactcttt
     7201 gatagactgc gccacgtcat tggaaaaaaa caaacgtttt ttctcaacat tgcgatccac
     7261 cacttcctta aggcccggct cataaatcgg tataacacct tgattgagtt tatcaatttt
     7321 ttctttatca atatcggcgc atatgacgtt gttgccaaaa tcggctaaac acgcaccgga
     7381 taccaggcca acataaccgg ttcctaccac gcatatcttt ttcacagtaa tacctctaga
     7441 ttattggatt ccgttatttt ttattctttg caaagcctct ttagcggctt gctgttctgc
     7501 ttcttttttg gataaccctc tgccgcatcc aagtatggta tctttaacgg ctacttgaat
     7561 ggtaaactct ttttgatgtt cgggccctgt ttcgctgacg acgatatact gcggaatacc
     7621 gagatctttg ccttgggtat attccagtaa atcgccctta aaattggaat tcatttcgct
     7681 ctgcaaaata cgctcggtat cgcaaagcag cgtatccaaa acaaatttac gcgccgtatg
     7741 atacccgtgg tccagataaa ccgctgcgat gagcgcttca aaaacatccg ataatataga
     7801 tgaacgcgaa cgaccacccg agcgctcttc accatcgctc atcactataa actgacccag
     7861 tcccaaggct tcggctttat gcgctaacgt ctttttattg actaagtgcg atttcccttt
     7921 cgtcaaaaag ccttcatcgg attcggggta tttctgaaaa agaaattccg ccgtgatcgt
     7981 actgagtatc gcatcaccaa gaaactccat gcgttcatac gacgaaaagt gtttctcatt
     8041 actcacattt aagaaagaac gatgtttgag agcttgtatg tataacttcg gattctcaat
     8101 ccgaacacct aaaacacgcg ataaatcctg aagccgtaat ccgttatcag gtaaaaaacc
     8161 atcggcttgt tcgggacccc aagcgcgccg gaaaaaacgg attatcccat taaaccagcc
     8221 gccatgtact gcatgctctt gtgaagtccc cggtgacata cagattacgg tttatacttc
     8281 gttacggcaa tacacgtatt gtgtccgcca aacccaaagg tattactcag cgctacgtcc
     8341 acgttacgtt gaatggcttt gttaggcacg taattcaggt cacacgccgg atccgatact
     8401 tcataattga tcgtcggcgg aatccacccg tttttcacgg ccaatgtggt cgcgataaat
     8461 tcgatgccgc cggccgcacc aagcaagtga cccgtcatgg atttggtcga actgaccagt
     8521 aacttatacg catgatcgcc gaaaacggtc ttgatcgctg cactttcgtt tttatcatta
     8581 tattcggtgg acgttccgtg ggcattgatg ta
//
LOCUS       DAOQGF010000127         8590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_3901_length_8590_cov_7.290334, whole genome shotgun sequence.
ACCESSION   DAOQGF010000127 DAOQGF010000000
VERSION     DAOQGF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_8590_cov_7.290334"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK937_13535"
     CDS             <1..332
                     /locus_tag="PK937_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNH33643.1"
                     /translation="MNFKVGGRRFYAMVSPEGQENWHIQDYTSISPKTNFKFFSVFAD
                     KDENPFLPGSNWNLNFNENKETTKVSITIQNDSLERMEQMIEMGFKEGFTMTLNELSN
                     LLSNLKK"
     gene            338..952
                     /locus_tag="PK937_13540"
     CDS             338..952
                     /locus_tag="PK937_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH33644.1"
                     /translation="MKKHGLKIGIALLIVNTLGCGNSQPNSNPISKIDSADNKILTSE
                     HSMKTQKITPCLWVDKDARAVVDYYLSIFKDGKMKDYRQYKNPPQENVPGGQDGFETA
                     IMEIGDIEFSILAAGPYFKFNESVSFVINCKDQAEVDYYWNALTTNGGEEGSCGWCKD
                     KYGLSWQVVPVEYFDLINSEDSKVREKAMRNTFKQKKLILSELK"
     gene            complement(1062..1292)
                     /locus_tag="PK937_13545"
     CDS             complement(1062..1292)
                     /locus_tag="PK937_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNH33645.1"
                     /translation="MKAYVLIKIKSGEVKDVVRQLRRIEGVMEAHMTFGPYDAVAAVE
                     SQDIARLGAITATAIQPIPGVEQTLTCIAVDV"
     gene            complement(1362..2249)
                     /locus_tag="PK937_13550"
     CDS             complement(1362..2249)
                     /locus_tag="PK937_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite family transporter"
                     /protein_id="HNH33646.1"
                     /translation="MSEIHIDALLPAEMATRAEYLGVRKAEMPALKMFTLAIMAGAFI
                     SLGAIFATTVSAGGVAITAPDGTTSLSTGLPYGVTRLLAGFVFCLGLILVVVGGAELF
                     TGNNLIVMAWASRKVTSYALLRNWVIVYAGNLIGALGTAVLMLWSRQYTFGSNSIGLS
                     AFKIAAGKCSLEFWQAMVLGILCNALVCLAVWLTYSARSTMDKIAAIIFPVTAFVAAG
                     FEHSIANMYFVPYVLLIQTFDPSYVASISDKMPGMYNLNWPNFITNNLIPVTIGNVIG
                     GAVLVAAVYWSVFLRNKDK"
     gene            complement(2522..4183)
                     /locus_tag="PK937_13555"
     CDS             complement(2522..4183)
                     /locus_tag="PK937_13555"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNH33647.1"
                     /translation="MISNHHSIARWVMEARSVLASARIMEIFCQQKNLLQIGLVTPSR
                     QSLVLEIYCDRQPYIVWRSSYSRKNKNSMDLFPMMIGLHIRDIVMDNTDRRVVLSFEN
                     GCSLVAQFYGSANVWATDASGVITGSFKNERAVHESSMKIESGGADMRRYTQDAVDVF
                     ESVALFREALSGGFGHLNKILTLEFLHAVSLSDITTAREAWRNFINAIYDGAPRIYTD
                     HGDLKYFSLLPLTHHLGRNPHWKEELFTSVNDAFLAYVQLRFFAERLQKKRDDLLRAS
                     RQRIKKNEHLIMALQEDAEKAATFAAHEQKAHLLNIHLRDVHRGMDKIVVTDVYDEAQ
                     NEIEIKLDPILSPQANVEKYFAQSKKLKQSVVKIAERILTIGKENDTLRQIEAQFADA
                     HAIERKALEKKYDEFVRNGWIKKTESSKPTRAADQEMPVFREFRVHGNWRVFVGQNDI
                     KNDALTFRFAKKDDLWFHARGVAGSHVVLKRDGRSDNPGQRAIEAAAQIAAFFSKAKT
                     SSLVPVAYTERKYLRKPKGAKPGMVVLEREEVLMVPPVEPREMAV"
     gene            4268..5680
                     /locus_tag="PK937_13560"
     CDS             4268..5680
                     /locus_tag="PK937_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33648.1"
                     /translation="MLSSFLSRFTFPLLFIFAAAIEFCMVRISGITSLRNDFESLYWH
                     FGIAFGIYLLTVLYVRFYWGKTFFSPEWIIGTAIVFRLTLLFSDPVLSDDIYRYIWDG
                     RVSNHAVNPYAYAPESGNLYGIRDSVIYPQINHKELQTIYPPAAQVLFRITSWISESV
                     LAMKLMVLLFDIGIIFLIMALLRHFELPLGWIAVYAWNPLAIIEFSGNGHMDAIGIFF
                     MLLAFWFSLREKTILSALPLALSFCTKYLTLPLLPFMEDIRSRWKNVLAITAIFAGTV
                     LLLYTGYSDVDAIKFGSLKIFSETWEFNASLYTVVENFFKTRLDSSLTTVWGISTDNP
                     ARLAARLTIGISLTLFIMILYGINFTKSYAVQTKRRIYYAFLIIAAILIVSPTVHPWY
                     VIWIIPFLCFYPNPAWVLFTGLVFMSYDTLVGSLFGAHWYERPEIKLWQYIPFYAVMI
                     IHFTWYLIRKFQSHPGEPVA"
     gene            5677..8133
                     /locus_tag="PK937_13565"
     CDS             5677..8133
                     /locus_tag="PK937_13565"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH33649.1"
                     /translation="MRTLSVKDWIIIIAGVIGLVVFILLYPKVYPESAMRGMISEESA
                     IAKSQQWMSSGWAKAPRSMDSLTLRASIRVNQKQLEYLQEKFGLEQANKHLRNKSLVY
                     TRQLEWLRIDEVNIQFGGRTQGNSSAKTERVVIELDRLGNIVGYRYTKAKQDTSPVTD
                     LETTHPSLAATEGFDLDSARRMAREFLRQWAGRSPDDFRESDVQGSITGKKPVMEFVF
                     TGIAPTDEVYERVKLRIENYRIANFEREWLYPAGFIDPENRWERETTNTLGMIFFILF
                     SIMVVVLFFRQFKRGGIDFKVGSFFGILSAVSFALMIGVQLLASGFWTLSFVVLIAGG
                     WYFIMTTGIIAVTASEVYRFWPESYRTFEAIRRGKIINRRWGRSLALGMAWTGITIGA
                     ISLVLYIVPGTSFLLNSKSTDKYDGYGALFLIGASIWTVLIHHHLFYLLPLSALRKRW
                     KNPAAVIGIAALVGILQPYIFDTVSPWEPRLLVGLLIGIIYGTILLNYDFLTLSVSAS
                     LAYLISESGFLYYTSDIPGMVIVGVYLFSFLTLALIALWSRDSGDDTLDYVPEYVKEM
                     ESKQRMNREFEIARTIQTKLLARTVPVNDRCDIACLCEPAHEVGGDYYDFVAFDDKNK
                     LGVVIGDVAGKGVSAAFYMTLVKGILQTQAPITSHSARETLERVNHIFCTQVERGRFI
                     SMVYGIFDFSKNTVTIARAGHNPVYRLRSGASQMDSLVPDGMAIGLTQNDTFAEKLKE
                     TTIAFSSGDVFVFFTDGFSEAMNKHGEEFGEARLQQTLLNVSGQNSSAMIDALQKEIR
                     SFVGQHPQHDDMTMIVIRIV"
     gene            8133..>8590
                     /locus_tag="PK937_13570"
     CDS             8133..>8590
                     /locus_tag="PK937_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33650.1"
                     /translation="MMYRFFISPYLSSYLAFVFWATVYALSSVVSVAIVMPFVNLVFS
                     PHTPLTPLISFPVMDRTTALWILCGTTFAVFLIKNFAYIAFQSIGARLQQKLITALRE
                     FRIHELLRMSYGAFLAENHGAMHHRLITTSETTVRAWMQWIFSLCQHFPLL"
BASE COUNT         2260 a         2031 c         1880 g         2419 t
ORIGIN      
        1 atatgaactt caaagtagga ggacgccgat tttatgctat ggtaagcccg gaaggacaag
       61 aaaactggca tattcaggat tatacttcca ttagccccaa aaccaatttc aagtttttca
      121 gtgtttttgc agataaagat gaaaaccctt ttttgccggg ctcaaattgg aatttgaatt
      181 tcaacgaaaa taaagaaaca accaaagtca gcattacaat tcaaaacgat tcgctcgaac
      241 gcatggaaca aatgatagag atgggcttca aagaaggttt cactatgaca ttaaacgaat
      301 tatcaaactt attatcaaat ttaaaaaaat aacgtatatg aaaaagcatg gacttaaaat
      361 tgggattgct ttattaattg ttaataccct gggttgtgga aattcgcaac cgaattcaaa
      421 ccctataagt aaaattgatt ccgctgataa caaaatttta acaagcgaac actctatgaa
      481 aacacaaaaa ataacacctt gcctttgggt tgataaagac gcaagagccg ttgtcgatta
      541 ttacctttcc attttcaaag acggcaaaat gaaagactat cggcaatata agaatccgcc
      601 gcaagaaaat gtgccaggcg ggcaagacgg tttcgaaact gcgattatgg aaattggtga
      661 tatagaattt agtattttag ctgcaggtcc atactttaag tttaacgaat cggtttcatt
      721 cgttatcaat tgcaaagacc aagccgaagt agattattat tggaatgcgt tgactaccaa
      781 cggcggagaa gaaggatctt gcggctggtg caaagacaaa tacggtttat cgtggcaggt
      841 agtacccgtg gaatattttg atcttattaa cagcgaagat tctaaggtaa gagaaaaggc
      901 aatgagaaat acatttaaac agaaaaaact tattttgtca gaacttaaat aataataaat
      961 cagatcatgc acacgaaaaa actgaatatt ttatcaattt cgtttttgta agcaaatccg
     1021 gcatctatgc ccacgctaaa ggcttagctg ataagcctca tttacacatc cacggcgata
     1081 cacgttaacg tttgctcgac acccggtatc ggttgtatgg ccgtcgcggt aatagcgcca
     1141 agccgtgcga tatcctgcga ttccacggcg gccaccgcgt catacggacc aaaggtcata
     1201 tgcgcttcca tcacgccttc gattcggcga agctgacgta ctacatcttt gacttcgccg
     1261 gattttattt tgatgagaac atacgctttc atacatactc cttatgattt gaatggacaa
     1321 catcacggta agcgcaatgg ttccctctaa gccaacccca ttcacttatc tttattacgt
     1381 aaaaaaaccg accaatacac cgccgcaact aaaacagctc ccccgatcac attgccgatc
     1441 gtcaccggta tgaggttatt cgtaataaaa ttcggccaat tgagattgta catacccggc
     1501 attttatccg aaatgcttgc gacataggac ggatcgaatg tttgaatcaa aagcacatag
     1561 ggaacaaaat acatattagc gatgctgtgt tcaaaacccg ctgcaacaaa ggccgtaacc
     1621 ggaaaaataa tggccgcgat tttatccatc gtactgcgcg cgctataagt gagccacacc
     1681 gcaaggcaga cgagcgcgtt gcacagaatg cccaatacca tcgcttgcca aaattccaaa
     1741 ctgcatttcc cggcggcgat tttaaaagcc gatagaccga tgctgttgga accaaaggta
     1801 tattggcggc tccacaacat gagaacggcc gtacccaatg cgccgataag gtttcccgca
     1861 tatacgatca cccaattgcg caacagtgcg tacgacgtca ctttgcggct ggcccacgcc
     1921 atgacgatga gattgtttcc ggtaaaaagt tcagccccgc caacgacaac cagaatcagg
     1981 ccgagacaaa aaacaaaccc ggccaataat cgtgtaacgc cgtaaggcag tccggtacta
     2041 agcgatgtcg ttccgtccgg cgccgtgatc gccacaccac cggcagagac ggtcgttgca
     2101 aagatcgccc ccaacgaaat aaacgccccg gccatgatgg ccagagtaaa cattttcaaa
     2161 gccggcattt cagcttttcg tacaccgaga tattcggcgc gtgtagccat ctccgccggc
     2221 aacaaagcat caatgtgaat ttcactcata gatatttctc cactgtaaaa tgtaataaac
     2281 cccgtcggta tgcgaacaaa cataccgcaa cttaccgcga ctaaaaaacg cgacggtgca
     2341 gcaacgcacg tatttttcaa tagcgtcgct ggataggggt gacggctgat cgggaaatat
     2401 ttgaataacg cgctgcatag caggcttgtg gtcttttaaa gaagcacttc aatttaatac
     2461 aatcggaggg ataaaactaa cagagatttt acaaaaaaat attcgcttag gccgccataa
     2521 atcagacggc catttcgcgg ggctccaccg gcggcaccat taaaacttct tcccgttcaa
     2581 gcacaaccat accgggtttg gcacctttgg gtttgcgaag atattttcgt tccgtgtacg
     2641 cgaccggcac cagcgatgac gttttggctt tgctgaaaaa cgcagcaatc tgagcagcgg
     2701 cttcgattgc gcgttggccc ggattatccg agcgtccgtc gcgtttgagt acgacatgcg
     2761 aacccgccac gccccgcgcg tgaaaccata agtcatcttt ttttgcaaaa cgaaacgtca
     2821 gcgcgtcgtt tttaatatca ttctgtccga caaaaacccg ccagttgcca tgcacccgaa
     2881 attcgcgaaa aacgggcatt tcctgatccg cggcgcgcgt cggtttggag gattctgttt
     2941 ttttgatcca accgttacgt acaaattcat catatttttt ttctaacgct tttcgttcta
     3001 tcgcatgcgc atcggcaaat tgcgcttcga tttgccgtaa ggtatcattt tctttcccga
     3061 tcgtgaggat acgttcggcg attttcacca cagactgttt gagttttttg gattgcgcaa
     3121 agtatttttc aacattggct tgcggcgata aaatcggatc gagctttatt tcgatttcat
     3181 tttgcgcctc atcatacaca tcggtaacga ctattttatc cattccccga tgcacgtcac
     3241 gcaaatgaat attcaaaaga tgcgcttttt gttcgtgcgc cgcaaacgtc gcagcctttt
     3301 cggcatcttc ttgcagcgcc atgatcaaat gttcgttttt cttaatccgc tggcggctcg
     3361 cgcgaagaag atcatctcgt tttttttgaa gacgttcagc aaagaaacgc aactggacat
     3421 aggctaagaa cgcatcatta actgacgtaa ataattcttc tttccaatgc gggtttcgcc
     3481 ccaggtgatg cgtcagcggc aacaatgaaa aatacttcaa atcgccatgg tcggtataaa
     3541 tacgcggtgc accgtcatag atcgcattga taaagtttcg ccatgcttcg cgtgccgttg
     3601 ttatatcgct taatgatacg gcgtgcaaaa actcaagcgt cagaatttta ttcaaatgcc
     3661 caaatccgcc ggataacgct tcacggaaaa gcgccacact ttcaaacaca tccacggcat
     3721 cctgcgtgta ccgccgcata tccgccccac ccgactctat tttcatggac gactcgtgca
     3781 ctgcgcgctc atttttaaac gatccggtta ttacacccga tgcatccgtc gcccacacat
     3841 tcgccgatcc atagaattgc gctaccaaac tacaaccgtt ttcaaaagat agaaccaccc
     3901 gccggtcggt attgtccatg acgatgtcgc ggatgtgaag accgatcatc attggaaaaa
     3961 gatccatcga atttttgttt ttgcgcgaat aactagaacg ccaaacgata tacggttgac
     4021 ggtcgcagta tatttccaaa accaatgact gacgggaggg tgtaaccaat ccgatctgta
     4081 gaagattttt ttgctgacaa aaaatctcca taatacgcgc ggatgccaat acggagcgtg
     4141 cttccatgac ccaacgtgcg atgctgtggt gattgctgat catgggaatt tactatttgc
     4201 ctttcggagt ctgtttctta aattgcccga caaattacaa cctcttgcct tagaagacaa
     4261 cgcctttatg ttatcctcgt ttttatcccg ttttactttt ccgttacttt ttatttttgc
     4321 cgccgcgatc gaattttgca tggttcgcat ttcgggcatc acttcattac gcaatgactt
     4381 cgagtcgctt tattggcatt tcggcatcgc attcggaatt tacctgctga cggtgttgta
     4441 tgtgcgcttt tattggggta aaactttttt ttcaccggag tggattattg gaacggcgat
     4501 cgtgtttcga ttgacattgc tattttccga tccggttttg tcggacgata tataccgcta
     4561 tatttgggac ggccgtgtat ctaatcacgc cgttaaccct tacgcttatg cgccggagtc
     4621 gggaaatctg tatggcattc gcgatagtgt gatttacccc caaatcaatc ataaagaatt
     4681 acaaaccatt tatcctccgg cggcacaggt tttatttcgc attacatcct ggatatccga
     4741 gtccgttctt gcaatgaagc tgatggttct gttgtttgat atcggcatta tttttctcat
     4801 catggcgttg ctccgtcact tcgagttacc gttgggttgg atcgcggtat atgcatggaa
     4861 tcctctggcg atcattgaat tttcaggcaa cgggcacatg gacgccatcg gtatattttt
     4921 tatgctcctt gcgttttggt tttccctgcg tgaaaaaacg attttatccg ctctgccgtt
     4981 agctttgtcc ttctgcacca aatatctcac actgcccttg ctcccattta tggaagatat
     5041 acgttcgcgg tggaaaaatg tattagccat aacggcgatc tttgcaggta cggtattatt
     5101 attatatacc ggttattcag atgtggatgc gattaagttt ggttcgctta agattttttc
     5161 tgaaacatgg gaattcaatg catcgttata caccgtcgtg gaaaattttt ttaaaacacg
     5221 cttggattca tccctcacga cagtctgggg tatttctacg gacaaccccg cacggcttgc
     5281 ggcgcgccta acgataggaa taagcctcac tttatttatt atgatattgt acgggatcaa
     5341 ttttaccaaa tcgtatgcgg tacagacaaa acgccgtata tattatgcgt ttttgatcat
     5401 cgctgccatc ctcatagtga gtccgaccgt acatccatgg tacgtgattt ggattattcc
     5461 ttttttatgt ttttatccca atccggcctg ggtgcttttt accggtttgg tatttatgtc
     5521 gtacgataca ttggtcggct cgttatttgg cgcacattgg tatgaacgac ctgaaatcaa
     5581 actctggcaa tacattccgt tttacgccgt catgatcatc catttcacat ggtatctgat
     5641 tcgtaaattt caatcgcatc ccggggagcc tgtggcatga gaacgttatc cgttaaagat
     5701 tggattatca tcatagccgg tgttatcggc ttagttgtct ttatactact ttaccccaaa
     5761 gtctatccgg aatcggccat gcgcggcatg atcagtgaag aatccgccat agccaagtca
     5821 cagcagtgga tgagcagcgg atgggccaaa gcaccgcgct ctatggattc gctcactttg
     5881 cgtgccagta tccgagttaa ccaaaaacaa ctcgaatacc ttcaggaaaa attcggtctg
     5941 gaacaagcca acaaacacct gcgcaacaaa tcattggtgt atacccgaca gttggaatgg
     6001 ttacgtattg acgaagttaa tattcaattt ggaggacgta cacaaggcaa ctcttctgcc
     6061 aaaacggaac gcgtcgtgat cgaactcgat cgcctaggga atatcgtcgg ctatcgttat
     6121 accaaagcaa aacaggatac ttcacccgta acggatttgg aaaccacaca tccatcctta
     6181 gccgcaaccg aaggatttga cctggacagt gcacggcgca tggcacgcga atttttgcgt
     6241 caatgggcgg ggcgctcacc ggatgatttc agagaatccg atgtgcaagg ttcgatcacc
     6301 gggaaaaaac cggtgatgga gtttgttttt accggcattg caccgacgga tgaagtatac
     6361 gaacgcgtga agcttcgcat tgaaaattac cgtattgcta attttgaacg cgaatggctt
     6421 tatccagccg ggtttattga tccggaaaat cgatgggaac gtgaaacgac caacacgctg
     6481 ggcatgatct tttttatttt gttttccata atggtcgttg tgttattttt tcgccagttc
     6541 aaacgtggtg gcattgattt taaagtcgga agtttttttg gtattctcag tgctgtcagt
     6601 tttgcattga tgatcggcgt acagctacta gcctccggtt tttggacact ttcctttgtc
     6661 gtgttaattg ccggcggttg gtactttatc atgacgacag gtattattgc cgtgaccgct
     6721 tcggaagtat atcgcttttg gcctgaaagt tatcgtactt tcgaagctat tcgtcgcggt
     6781 aaaataatca atcgtcgctg gggccgcagt ttagcactcg gtatggcatg gacaggcatc
     6841 acgatcggcg ccatttcact tgtattatac atcgtacccg gcacttcgtt tttactaaac
     6901 tccaaaagta cggacaaata cgacggatac ggggcattat ttttaatcgg cgcttcgata
     6961 tggaccgttc tcatacacca tcatttgttt tacttgcttc cgctgtccgc tcttcgcaag
     7021 cgctggaaaa acccggccgc cgtcatcggc atagctgcat tggtcggcat tttgcagcca
     7081 tacatttttg ataccgtttc accgtgggaa ccgcgtcttc ttgtcggttt attgatagga
     7141 atcatttacg gtacgatttt attgaattac gattttctga cgctgtccgt atcagcaagc
     7201 ctggcctatc ttatttccga atccggtttt ttgtattaca catcggacat tccgggcatg
     7261 gtgatcgtcg gtgtctattt gttttcgttt ctgacactcg cgctcatcgc gctgtggagc
     7321 cgtgacagcg gcgacgatac gctggactat gtcccggaat acgtcaaaga aatggaatcc
     7381 aagcagcgca tgaatcgcga atttgaaatc gcacgaacga ttcagaccaa actgctcgcg
     7441 cgcaccgtac cggtcaacga tcgctgcgat atagcttgtc tatgcgaacc cgcccatgaa
     7501 gtcggcggag actattatga ttttgtagcg tttgacgata aaaataaact tggcgtggtc
     7561 atcggggatg tcgccggcaa aggcgtttcg gccgcttttt atatgacatt ggtcaaaggc
     7621 attttgcaaa cacaagcgcc gatcaccagc cattcggcgc gtgaaacttt agaacgggtc
     7681 aatcatattt tttgcacgca ggtagagcgc ggccgtttta tcagtatggt gtatggtatt
     7741 tttgatttct ccaaaaacac cgtcacgatc gcacgcgccg gtcataaccc cgtgtatcgt
     7801 ttacgcagcg gtgcttcaca gatggattcg ctcgtaccgg acggcatggc gatcggcttg
     7861 acgcaaaacg atacatttgc agaaaagctc aaagaaacca cgatagcttt ttcctccggc
     7921 gatgtttttg tgttctttac ggatggtttc tccgaagcca tgaacaaaca tggtgaagaa
     7981 ttcggcgagg cacgacttca acaaacgtta ctcaacgtat caggccaaaa ttcgtctgcc
     8041 atgattgatg cattgcaaaa ggagattcgt agttttgtgg gacagcatcc gcagcatgac
     8101 gatatgacca tgatcgtgat tcgtatcgtg tgatgatgta tcgttttttt attagccctt
     8161 acctgtcatc gtatttagcc tttgtatttt gggctaccgt atatgcgttg agctcggttg
     8221 tcagtgtggc tatcgttatg ccgttcgtca atctcgtttt cagtccgcat actcccctta
     8281 caccgcttat ttcttttccc gtcatggatc gcaccaccgc tttatggata ttatgcggga
     8341 cgacttttgc tgttttttta ataaaaaact tcgcctacat cgcattccaa tccatcggcg
     8401 cacggttaca acaaaaactt atcacggcgc ttcgcgaatt tcgaatacat gaactgctgc
     8461 gcatgtctta cggcgcattt ctcgcagaaa accacggcgc gatgcatcac cgcctgatca
     8521 ccacgtccga aaccacggta cgcgcatgga tgcaatggat tttttctctt tgtcagcatt
     8581 ttcctttact
//
LOCUS       DAOQGF010000128         8252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4115_length_8252_cov_6.457850, whole genome shotgun sequence.
ACCESSION   DAOQGF010000128 DAOQGF010000000
VERSION     DAOQGF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4115_length_8252_cov_6.457850"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK937_13575"
     CDS             <1..973
                     /locus_tag="PK937_13575"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNH33651.1"
                     /translation="EGLRAIIDQSRKQGLTYITDTDARPRGTAHPTAHLWDNGTNAAD
                     ELLRIMKIRAKALDNFSDRVIRVGEPNSTIEEVLVPIYLLHRYQTDAAAKIIGGLHYE
                     YTLRGDGSVPAYPATDQEQERALSAVLSTIETSALTLPEHIIKIIPPRADGYDMHREL
                     FPRRTGVMLDPLTIAETAAEHTISVLLHPQRMNRLLEQKRREVSKIDAAAVLDSLINR
                     TFKKQVANAAEAEVQRTINYVALYQLFYLANSGEAQPQIQAITWSKINALGQWVANQK
                     TTGAWKAHYEGIARLIREFNDEPKSFVLPYKPKPAPPGAPIGSCDFE"
     gene            complement(1026..1442)
                     /locus_tag="PK937_13580"
     CDS             complement(1026..1442)
                     /locus_tag="PK937_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNH33652.1"
                     /translation="MDMASAFNSLNWLAIIVASISAFVLGGIWYSPLMFGNAWMKVAG
                     MTEESVKQGNPGKIYGGAFLLTLIAAINLGMFLGAQSDMAFGIAAGAATGIGWVAPAF
                     GVVYLFEQRPTLQWFINGAYWVVTFIVMGAIIGAWH"
     gene            1636..2706
                     /locus_tag="PK937_13585"
     CDS             1636..2706
                     /locus_tag="PK937_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH33653.1"
                     /translation="MEPLNEENIDQELNDRTIRVIMIPFFGIVIPNITGMIDNSAYTV
                     NQLVLHYLYFMFIAFAIWHGNRYLLFRLRIRFSWLSQPMYKVLALMVSNMFYTVPIAV
                     GLMTAWYVFAQHRSADWDVIFKATALCVVCVVFITHTYETVFLIKSWETDKTKSEALE
                     KAKAQAELEALKNQIDPHFMFNSLNTLSHLIEQDPPKARMFNDNLAEVFRYILYNRNR
                     NLVLLSEELSFAEKYFSLLKIRFGESIGLHIAEDNVSPDNVLIPPISLQILLENAIKH
                     NTFSDQQPMAITIRVSDQAVVVKNPIRKKVQVKNSSRTGLENLNERYKLIVGQTIHIE
                     ENVENFTVHLPVLMTGAHAVPY"
     gene            2721..3473
                     /locus_tag="PK937_13590"
     CDS             2721..3473
                     /locus_tag="PK937_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH33654.1"
                     /translation="MNVLIIEDEKPAGERLVAMVHDYDPAIRIVATLHSVKEARGWFA
                     KHNAPDVILADIQLNDGLSLEIFKDTAIHAPIIFTTAFDEYLIKAFEFNSIDYLLKPI
                     QKPKLFHALDKYVRLRQHFTGSLSDLLAQMSRSSEPSLQRIVVQKGTDFIAVPINDVA
                     YFFTEHKMVFLVDARGKKFIVDKPLADLEAALDKQTFFRLNRKYIARISAIVRFKPYE
                     KGKIKVELQPGTSEDIIVSQENAADFKNWMGK"
     gene            complement(3521..3940)
                     /locus_tag="PK937_13595"
     CDS             complement(3521..3940)
                     /locus_tag="PK937_13595"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNH33655.1"
                     /translation="MPLIKRSAQAHWAGTGKEGKGDLSTQSTVLNKTQYSFGTRFENG
                     VGTNPEELIGAAHAGCFTMKLSFVLNAMGLTAEALDTKATVSFEDGVVTQVHLELTGK
                     VPGCTAEKFQEAANDAKANCPISKLLNTTITLDAKLV"
     gene            complement(4010..4609)
                     /locus_tag="PK937_13600"
     CDS             complement(4010..4609)
                     /locus_tag="PK937_13600"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNH33656.1"
                     /translation="MLFSKTRTEALSDGVFAIVITLLVLEIKVPDMHASGQSLTHALV
                     GLLPKIISWVISFAVVMIFWINHHRLFHSLTSVDARCMGLNGIFLMGLSFIPFPTALM
                     GEYIHEALAVSLFGWIMALTAGVLPILRSYASKAGLLDPMHDKQTIKTYNRRSYILGS
                     GAYAVAALLAWIEPIIAIVLYAVIAMYFIRPKHRHLKHV"
     gene            complement(4626..5414)
                     /locus_tag="PK937_13605"
     CDS             complement(4626..5414)
                     /locus_tag="PK937_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNH33657.1"
                     /translation="MKVFWIAVLINTVCVFGQHDPNTSSPKKLILMVAANSSVSAQTG
                     WPIGVWAAEITHPYLAFIEAGYEVEIVSPNGGKIEFDNYSDPRDPSGYSAHDMISMGF
                     IHTPSHMALLEKTRRLDEINEKNYDAIFVCGGQSPMYTFIDNTKLHTLFAKFYESGKP
                     SAAICHGTCILLKAKLSNGQYLIEGKTWTGFANSEEDFADKAVGKKIQPFRIESEAGK
                     MNNTNFITHNAFRPFAIRDGHLITGQQQNSGTEAAKLVIEMLGY"
     gene            5570..5911
                     /locus_tag="PK937_13610"
     CDS             5570..5911
                     /locus_tag="PK937_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH33658.1"
                     /translation="MPEFTANGKRYNNPVEFALGKIGGKWKIPILWRLKDRIWRYGEL
                     KRDLKKITHKMLSEQLDEMEADGLICREVFPEVPPRVEYSLTNKGRTVVPIIEALREL
                     GLAMMKSENIE"
     gene            5968..6882
                     /locus_tag="PK937_13615"
     CDS             5968..6882
                     /locus_tag="PK937_13615"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNH33659.1"
                     /translation="MNKVYTNAESIDFRLADIPERPEPEKLLMCSPDHFNVTEAINNF
                     MTDTAGQMNQVDRPLAFQQWTNIRFLYEALGYRVHTIRGEVGLEDMVFSANQSFPFWD
                     RHQEKPAVIMSNMRKDKRKGEVPYFQSWYRKQGYMIHELKGGCFESNGDALWFPEKHL
                     IISGFGSTTHHRTDLLALEQVARITDGPVVGIELKHPDFYHLNTTLAIIDKDVCLVYP
                     PGVGDHGMAILRLFFKTIIEVSKEEAYAPNFACNAFSPDGEIVFIQGTCVETIHALEK
                     LGKRVIGVDTSEFIKSGGSVFCMKIKVY"
     gene            7033..7935
                     /locus_tag="PK937_13620"
     CDS             7033..7935
                     /locus_tag="PK937_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33660.1"
                     /translation="MESFWPETPLLNAIPAQTITLSDLPVRSVISPDGDYVIVYDDST
                     LVVYNTISWRIETKMNMDSGLRRVCYGADGHHIALETSAGREIWDTKTWEVLYKEENA
                     DSLSIVLPSSQGFAKAKTQVEGDDWALIVQSWPYRQQFAKLNAPEERPLWTMDITPHL
                     IAMYHHDQKKITITEIKTGRIVDTLLAKRPDKIKLSPNGRYLAQIYNGAVRVYDRWSN
                     EEKYYGQRIIFGEPAGIFSADSNYVLIAGNGNVKMIELATGEVRLDLATRFHMVRSLE
                     LSKDNKLLTITDADERSVTVYRIG"
BASE COUNT         2334 a         1746 c         1892 g         2280 t
ORIGIN      
        1 agaaggatta cgtgcgatca tagatcaaag ccgcaagcaa ggtctcacgt acatcacgga
       61 tacggatgca cggccgcgcg gcacagcgca tcccacggcg catctttggg ataatggtac
      121 caatgccgcg gacgaactcc tacgtattat gaagattcgt gcgaaagcac ttgataattt
      181 ttcagatcgc gtaattcgcg ttggcgaacc caacagtacg atcgaagaag tattggtacc
      241 gatctattta ttgcatcgtt atcaaaccga tgcggctgct aaaatcatag gtggcttgca
      301 ttatgaatat acgctgcgtg gtgacggaag tgtgcccgcg tatccggcaa cggatcaaga
      361 gcaagaacgc gcattatcgg cagttctcag taccatcgaa acttcggctt taactttacc
      421 cgaacatatc attaagatta tccctccccg tgcggatgga tacgatatgc atcgcgaatt
      481 attcccgcgc agaaccggtg ttatgcttga tcccttgacg atcgcagaga cggccgccga
      541 acatacgatc agcgtacttt tgcatccgca acgtatgaac cgtttactgg aacaaaaacg
      601 ccgggaagtt tctaaaatag atgctgcggc cgtgttggat agccttatca accgcacctt
      661 caaaaagcaa gttgctaatg ccgctgaagc agaggtacag cgcacaatca attatgtagc
      721 tttgtatcag ttattttatt tggctaattc cggcgaagcc cagccgcaaa tccaggcgat
      781 aacatggagt aagatcaatg ctctggggca atgggttgcc aaccaaaaaa ctaccggtgc
      841 atggaaagcc cactatgaag gaatagctcg attgattcga gaatttaatg atgagccgaa
      901 aagttttgta ctgccttaca agcctaaacc agcaccgccg ggcgcaccga ttggttcgtg
      961 tgattttgaa tagaataaaa aaaccacggt tagttgccgt ggttttttta tatctataaa
     1021 ctttgttagt gccatgcacc gatgatcgca cccatcacga taaatgtgac aacccaatag
     1081 gcaccattga taaaccactg cagtgtcggg cgctgctcaa aaagataaac cacaccaaac
     1141 gcgggagcaa cccagccgat acccgtcgct gcaccggccg cgataccaaa cgccatatcg
     1201 ctttgggcgc cgaggaacat tcctaaattg atcgccgcga tcaatgtcag taaaaaagca
     1261 ccgccgtaga ttttaccggg atttccttgt ttgacggatt cttcggtcat accggccact
     1321 ttcatccagg cattgccgaa catcaatggc gaataccaaa taccgccgag cacaaaggcc
     1381 gaaatactgg ctacgataat ggccagccaa ttcagtgaat taaatgcact tgccatgtcc
     1441 atacatcatt cctccgtttg aataaaaaaa tgagggtatt tttttaaata cgatcgcaag
     1501 ttaaacgcaa tatggaggca ctgcaaaggt tattctactc aaaccgaaaa aaggcgaact
     1561 gaaaccggaa aatacgaagc tgaatcggag tgaaattttt gcacaaagca tgaatagtat
     1621 gtagattggc aaatcatgga gccgctaaac gaggaaaata ttgatcagga attgaatgat
     1681 cgcacaatac gtgtgattat gattcctttt ttcggcatag tgattcccaa tatcacaggc
     1741 atgattgata atagcgccta taccgttaat caactcgttt tgcactatct ctattttatg
     1801 tttatcgcgt ttgcgatttg gcatggtaat cggtatttgt tatttcgttt acgcattcgg
     1861 ttttcatggc tcagtcagcc gatgtataaa gtgctcgcgt tgatggtttc caatatgttt
     1921 tataccgtac cgattgcggt cggactgatg acggcttggt atgtttttgc gcagcaccgc
     1981 agtgcggatt gggacgtgat tttcaaagcg acagcgctat gcgtggtgtg tgtggtattt
     2041 ataacgcata catatgaaac cgtctttctt atcaaatcat gggaaactga taaaaccaaa
     2101 agtgaagcgc ttgaaaaggc caaagcacag gcggaattag aagcgttgaa aaatcagatt
     2161 gatccgcatt tcatgttcaa ttcgcttaat acgctttcgc acctgatcga acaggatccg
     2221 cccaaagcgc gtatgtttaa tgacaatctt gcggaggttt ttcgttatat tttatataac
     2281 cgcaatcgta atttggtttt actcagcgag gaactctctt ttgcagaaaa atatttttcg
     2341 ctgctgaaga ttcgttttgg tgaaagtatc ggtttgcata ttgcggagga taatgtttct
     2401 ccggataatg tgctgattcc gccgatatca cttcaaattc tattggagaa tgcgatcaaa
     2461 cataatacgt tttccgatca gcagccgatg gctatcacga ttcgggtatc cgaccaagcg
     2521 gtcgtggtga agaatccgat aagaaaaaaa gttcaggtca aaaattcgtc gcgcaccggg
     2581 cttgaaaacc ttaatgaacg ttacaagttg atcgtcggac aaacgatcca tattgaagaa
     2641 aacgtcgaaa attttacggt tcacctgcct gtactgatga ccggtgcaca tgcagtgccg
     2701 tactaaacga tccatgttat atgaatgtac tgattattga agacgaaaaa cctgcgggtg
     2761 aacggttggt tgcgatggtg catgactacg atcctgcgat tcgtatcgta gcgacgctcc
     2821 acagtgtaaa agaagcacgg ggttggtttg caaaacataa tgcacccgac gtgatattgg
     2881 cggatattca gctcaatgac ggattatcgc tggaaatttt caaggatacg gcgatccatg
     2941 cgccgatcat ttttacaacg gcgtttgatg aatatttgat caaagcattc gaatttaact
     3001 ccatagatta tcttctcaaa ccgatacaaa aaccgaaact gtttcacgcg cttgataaat
     3061 acgtgcggct tcggcaacat ttcaccggca gtctctccga tctactggcg cagatgagtc
     3121 gttcttccga gccttcgttg caacgcatcg tcgttcaaaa aggaacggat tttatcgcgg
     3181 tacctataaa tgatgtagct tattttttta cagaacacaa aatggttttt ttggttgacg
     3241 ctcgtggtaa aaaatttatc gttgataaac cgttggcaga cttggaagcg gctttggata
     3301 aacaaacctt tttcaggctc aatcgaaagt atattgcacg tatttcggcg atagttcgat
     3361 ttaagccgta cgaaaaagga aaaatcaaag ttgaattgca gcctggtaca tcggaggata
     3421 ttatcgtgag ccaggaaaac gcagccgatt ttaaaaactg gatgggcaaa taaaaaaacc
     3481 acgccgaagc gtggttttat acattcatat tgttttggag ttaaaccaat tttgcatcca
     3541 aagtgattgt ggtattcagc aatttggata tcgggcagtt ggcttttgca tcattggcgg
     3601 cttcttgaaa cttttccgct gtgcagccgg gcactttacc ggtaagttca agatgtacct
     3661 gcgttaccac accgtcctca aaagaaacgg tagctttagt gtcgagggct tcggcagtta
     3721 atcccatagc gtttaataca aaacttagct tcatcgtaaa gcaacccgca tgcgccgcgc
     3781 cgatcaattc ttccggattg gtaccgacgc cgttttcaaa gcgcgtccca aacgaatatt
     3841 gtgttttatt cagtacggtg ctttgcgtgg acagatcgcc tttaccttct ttgcctgtgc
     3901 ctgcccagtg ggcttgtgct gaacgtttaa tcaacggcat aactagttct ccttgtttga
     3961 cctaagttgg catagtatac ggatcgtcgc ctattacgtg caatgcaaat catacgtgtt
     4021 ttaaatggcg atgtttgggg cgaataaaat acatcgcaat cacggcgtat aaaacaatcg
     4081 cgataatagg ctctatccac gcaagcaagg ccgcgacggc ataagctccg ctgccaagta
     4141 tatatgaacg ccggttatat gtcttaatgg tttgtttatc gtgcattgga tccaacaggc
     4201 ctgctttgga tgcgtaactt cgaagaatag gcaatactcc ggctgtaagc gccatgatcc
     4261 atccaaaaag actaacggct aatgcctcat gaatgtattc gcccatcagc gcggtcggaa
     4321 atggaataaa agataatccc ataagaaaaa tcccgttaag acccatgcaa cgcgcatcga
     4381 ccgaagtaag gctgtgaaat aaccggtgat gattgatcca gaaaatcatc accacggcaa
     4441 aactaataac ccaactgata attttgggta gcaggccgac taacgcatgg gttaacgatt
     4501 gaccggatgc atgcatatcc ggcactttga tttcaagtac gagcaatgta atgacgatag
     4561 caaaaacgcc gtcactgagg gcttcggttc gtgttttgga aaataacata atgtactcaa
     4621 aggtattagt agccaagcat ttcgatgact aattttgcag cttccgtgcc gctgttttgt
     4681 tgctggccgg tgataagatg cccgtcgcga atggcaaaag ggcggaacgc attgtgagtg
     4741 ataaaattgg tgttattcat ttttccggcc tcgctttcaa tacgaaatgg ttgaattttt
     4801 ttacccaccg ctttatcggc aaaatcttct tcgctgtttg caaacccggt ccatgttttt
     4861 ccttcgatca gatattgacc gttgcttaat ttagccttaa gtaagatgca cgtaccgtgg
     4921 catatcgccg ccgacggttt accggattcg taaaatttgg caaacagagt atgcagtttt
     4981 gtgttgtcta taaatgtgta catgggtgat tgaccgccgc atacgaagat cgcatcgtag
     5041 tttttttcat ttatttcatc caaacgacgg gttttttcta acagggccat gtgcgatggc
     5101 gtatgaataa aacccatgct gatcatatcg tgcgccgaat agccgctcgg atcgcgcgga
     5161 tcactgtaat tgtcaaattc aattttaccg ccgttagggc tgacgatttc aacttcgtaa
     5221 ccggcttcga tgaaagctaa gtacggatgc gtgatctcgg ccgcccacac gccgatcggc
     5281 catccggttt gtgccgatac cgaagaattt gctgcgacca ttagtatgag ttttttaggt
     5341 gacgatgtgt tgggatcatg ctgaccgaaa acacaaacgg tattgatcaa tacggcgatc
     5401 caaaatactt tcattataga ctcctcgtgc gttttgttga gcataaaaat gttttgtact
     5461 cgataaatat aatagctaac tttcgttcgt caagaacgca cctttgggtg tgatacgcac
     5521 ttttgagtga gtattggtgt tattgtttta cttacgtatc aaaaaaaaca tgcccgaatt
     5581 tacagccaac ggtaaacggt ataataatcc cgtagaattt gcgcttggga aaatcggagg
     5641 aaaatggaag atcccgattc tttggcgtct caaagatcgc atatggcgtt acggcgagtt
     5701 aaaacgtgat ttaaaaaaaa taacccacaa aatgctgagt gaacaactgg atgaaatgga
     5761 agccgatggt ttaatttgtc gcgaagtgtt tcccgaggtg ccgccacgcg tcgaatacag
     5821 ccttacgaat aaaggccgca cggttgttcc aatcatcgaa gcactacgtg agctcgggct
     5881 ggcaatgatg aaaagtgaaa atatcgaata acgtgggact tacggatgat ctgtgatctt
     5941 atctacaact aaactagttt tgatgatatg aacaaagtat ataccaacgc ggagtctatt
     6001 gatttccgtc tcgccgatat tccggaacgt ccggaacctg aaaaactttt gatgtgctcc
     6061 cctgaccatt ttaatgtgac cgaagcgatt aataatttta tgaccgatac cgccggtcaa
     6121 atgaatcagg tggatcgccc gctcgcgttt cagcagtgga ccaatattcg ttttttatac
     6181 gaagctttag ggtatcgtgt acatacaata cgcggtgaag tagggcttga ggatatggtt
     6241 ttttcggcca atcaaagttt tcctttttgg gatcgccatc aggaaaaacc cgcggtgatc
     6301 atgagtaata tgcgtaaaga taagcgcaaa ggggaagtgc catacttcca atcatggtat
     6361 cgcaaacaag gctacatgat tcacgaatta aaaggcggct gtttcgagtc caacggtgac
     6421 gcgttatggt ttcccgaaaa acatctgatc atttcaggtt tcggaagtac gacgcatcac
     6481 cgcaccgatt tgctggcgtt ggagcaagtg gcgcgtatta ccgatggacc ggtggttggg
     6541 atcgagttga aacatccgga tttttatcat ctcaatacta cattggcgat catagataaa
     6601 gatgtatgcc tcgtttatcc gcccggtgtc ggtgatcatg gcatggccat cctgcggcta
     6661 ttctttaaaa ctattatcga agtcagtaaa gaagaagcgt atgcgcctaa ttttgcatgc
     6721 aatgcatttt caccggacgg agaaattgta tttattcagg gcacgtgtgt ggaaacgata
     6781 catgcattgg aaaagctggg caaaagagtt atcggtgtag atacttcgga atttatcaaa
     6841 tccggcggtt cggtattttg tatgaaaata aaagtgtatt aaaaacgcaa cgtccgcatc
     6901 tgcggcacgc cgtacaaaaa aacttcatat aatgttattc ttatgactca ttcgatgcgc
     6961 agaatgcttg ggttcacggt aggtggtgtt tttttatttt tattttttct tgccccgcta
     7021 tacggatatt tgatggaatc gttttggccg gagactccgt tgttaaatgc tatacccgcg
     7081 cagacgataa cattatccga tctgccggta cgctcggtga tcagtccgga cggagactat
     7141 gtcattgtgt atgacgattc gacgctggtg gtgtacaata cgatttcgtg gcgcatcgaa
     7201 accaaaatga atatggatag cggattgcgt cgtgtgtgtt atggtgccga tggtcatcat
     7261 atcgcattgg aaacatcggc ggggcgtgaa atctgggata ctaagacctg ggaagtcttg
     7321 tacaaagaag aaaatgcgga ttcgctttct atagtacttc cttcatcgca aggttttgca
     7381 aaggcaaaaa cgcaagtgga aggtgacgat tgggcgctga ttgtccaatc ctggccgtac
     7441 agacaacagt ttgcaaaact caatgcgccg gaagaacgtc ctttgtggac tatggatatt
     7501 acgccgcatc tcatcgcgat gtatcatcat gatcaaaaga agatcaccat aacggaaatt
     7561 aaaaccggtc ggatcgtaga tacgttgctt gcaaagcgtc ctgataaaat aaaactcagc
     7621 cccaatggac gttatctagc gcaaatttat aacggagcgg ttcgcgttta cgatcgctgg
     7681 agcaatgaag aaaaatatta tgggcaaaga attatattcg gagagcccgc ggggatattc
     7741 agcgcagatt ctaattatgt gcttatagca ggaaatggta acgttaaaat gatcgagctt
     7801 gcaacaggcg aagtccgact tgatttggca acacgctttc atatggtacg gtcgcttgaa
     7861 ctctccaaag ataataaact cctgaccatt actgacgccg acgaaagaag tgttactgtt
     7921 tatcgtatcg gctaacgtgc catcttttta ttgcgtctgt gaatactaga tacagtttta
     7981 ctcgatcgta aaattatttt ccatctccct ccttgtttta tttaatcaat ttttcggcat
     8041 agggtttgtt tcacacaaat cagacgtatt taaactcttt atcggagaat tattaaaatg
     8101 aaaactatcg tttttatcag ctgttttttt ggtttaaatc tttgggctat gccagcagat
     8161 tcatcacgta gtgtgattat taaactcgga ttgaatgctt cgtatccaca aatgaatgga
     8221 aaaacatcgc ttcattccaa aaaaaatgct ac
//
LOCUS       DAOQGF010000129         8081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4239_length_8081_cov_5.022801, whole genome shotgun sequence.
ACCESSION   DAOQGF010000129 DAOQGF010000000
VERSION     DAOQGF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_8081_cov_5.022801"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PK937_13625"
     CDS             complement(<1..1545)
                     /locus_tag="PK937_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH33661.1"
                     /translation="MKNLIYYLVTAIALTWGNTSYGQGISSDANTLGLWRLDEASGTT
                     ITDASGRGHHGTTGGNPTVVSGRYNNARSFITDSIVVPDHADWSFGSNPFTIECWVNF
                     SSNASHGIIVGQIGSGSDYWRLHYEISANKLEFVYPGSTTQYVWTPVTDQWYHLAVTK
                     SDSVKIYINGIKVASGSIGSPGTVPNLSRPLIFGWGESNYYFKGSIDEIRISNIARSP
                     AEFNLAPAAPANMTATAVSTSQINLSWTASTGGPTKYRIYRSDGIDNNYVQRDSISSP
                     SANSYSATGLGTATTYYFKVAAVNEFGETRSAAAFDTTESAGISFTESSVGSTLFSLG
                     SPLDLGDYDNDGDLDILVAGNNGSTIATKLYSNQNGSFTDELSSIDNLYAGGVHFGDY
                     NNDGFLDFAIMGSTSNYDPPTSGGDPRLKIYKGNGTSAFTVDTSLSPVAMGNIVMPIR
                     WGDYDFDGKQDLLAAGLLYRNEGGTGRTFSRVGFLPDSNRESSVAWCDYDRDGDQDFV
                     YIAGVYV"
     gene            complement(1652..2278)
                     /locus_tag="PK937_13630"
     CDS             complement(1652..2278)
                     /locus_tag="PK937_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNH33662.1"
                     /translation="MRQIGWSVVLMLIVFGIGIYGQKQEKERKPTAAVLDFKIISGLT
                     PQEAAALTSKFRNSFSNTGKYTVLERGEMDKILKEQDFKMSDVCNSSECAVEIGQLLA
                     AEKMVTGDIGKIGETYSVTIKLLDVTTGKVDVTINEEYRGKPDGLLTVFDVMAQKITG
                     TYRVSKTKWYILGGVVVAGGAAAAVLLSGGKSGTTASGFPNPPSPPKQ"
     gene            complement(2516..2821)
                     /locus_tag="PK937_13635"
     CDS             complement(2516..2821)
                     /locus_tag="PK937_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33663.1"
                     /translation="MSKNDKYDATREFIDVLTEIIAEPHQHVLDKVILICTRLRGSYV
                     PSEKYVLRQKAVRDVIEMHHRGMPVRLIIEKTGLKKSTVYKIIKDPNGGISEKNNTK"
     gene            3051..3701
                     /locus_tag="PK937_13640"
     CDS             3051..3701
                     /locus_tag="PK937_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH33664.1"
                     /translation="MDSDQLSGTLLKAIDFQLDNADAVNDRFVQWRLIGTANDGEWVQ
                     TWVYCWTRRYFFRKFLCGQIHSAGDTEKLIEEVYFLFIQKHAHVHTPSHFAAWLSVIC
                     KYRFLNYIRSCHPEHWNEALTEVVGNEADKSIVHDLDVKLFFDRIISGEMRQLPDYVQ
                     TVIHKKIWEDKEYHVIAQEMGYSVETIRAYFARGIKALRNSHTIREAYAESYEPNP"
     gene            3726..4352
                     /locus_tag="PK937_13645"
     CDS             3726..4352
                     /locus_tag="PK937_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33665.1"
                     /translation="MFDSNEKLSRQEIADYAMRGHFPLTPEKEESIRRRIDMDPDAQT
                     ELQVMLERKAGLDRLTPSENHFARLQKKTTPSIKSHFSKTLWLRAAAIFLIIVGSYAG
                     LVTYVNYRFDHGPQGLAQFQKDYEFAIAPAATRGSNEKEPEDIRLFKEGVRLLFESKE
                     TTFGLSPRYDEESISHVIEIFEKVEQITENTDLRNRASLIVEKCKSFN"
     gene            complement(4363..4983)
                     /locus_tag="PK937_13650"
     CDS             complement(4363..4983)
                     /locus_tag="PK937_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33666.1"
                     /translation="MKKIGTLTIIVLMAAWLIYLNWPKGTVQILGVDNSGVGLPPPPC
                     PNCLTVESGARPAIGTEKIIIAIPQNSLFEMSLACDALLLDKPGSNKLLFLRFKQGNK
                     PTLEFRNTTAWPGNHFQGLIDNINSTSSKRDYIVLIQSDHIMRITKGNMVMTDFNTIA
                     KELNMRDSSNYGFYREKHVNNLNSGISDKIEIEIEGSGFVKKMDLL"
     gene            5006..7504
                     /locus_tag="PK937_13655"
     CDS             5006..7504
                     /locus_tag="PK937_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH33667.1"
                     /translation="MKVLEHNNYIFFLVGFHFIVNSLGFSQSATLSEINKMIDGGDLS
                     HASILLDSLLHQSITKKDSLGIGYGYLRLGLCYQNLNDFEKAIELNSHAIPIFLGLKD
                     TLYYAWSLVTEAHYSLIANRFHFVLRNLENVLNLCNATPKLKSIYYDALFRTGAFYLK
                     IGNFDQSIKTLKKAENYFESTYDRFTLSAVLFNMGAAYKETGDYKLSETCLRRVLTLA
                     AGIKQPNDRAKAAYSMLSDLYLSIGKLDQAIAMEYLAIKELKTEKAEADILFCDLGNL
                     FLRLKDFQKGEDAFLKGLSYLATNWNLHSGLAYIAEIRGNIAQADSFHSRAITLANQH
                     IKYTQGTATSAILKKSIPTFNQAARFYWRIGKPEKAFELLETGRARYLSQLLNLANVK
                     SDKSAFTDSIRNIEKQIGELYRKPESGLSTHQLQIFALEAAREQLLDSIKKYSPQFYA
                     FKKTKIPTLKELQTSLGSEFQLIEYAVMDDSIFALTVNSEKVSGHVMQIRRDSLERMI
                     DRVITAKQSSLHDLQQLYWLLITPMEKNLQTGKPLIIIPDDALFRLPFEMLVKNHDGN
                     YRSAKYLLHDFPVSYAVSASVWLDRLGRHSRAPKNYAGFAAAVFDTLSALPFVKNQVD
                     DAADRFSNSTSWTDAADLRRLFVNRAGKYKIIDMATHAFVSDIEPMRSKIVFNEHGGE
                     MNAFEIYELDLDADLAVLSACGTGLGKLSAGEGFMGFNQAFSYAGVYSLIMSAWDIDD
                     ESTAMIMKSFYEGIASGLTRAEALRRAKLKYINEAYELKQSPYYWAGLMLWGNPEAIP
                     LHRVPYGFIAFLFIPFAIIIVSPFVGRLRKKKKH"
     gene            complement(7843..>8081)
                     /locus_tag="PK937_13660"
     CDS             complement(7843..>8081)
                     /locus_tag="PK937_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH33668.1"
                     /translation="KEEALTAITKSPAEILGINDRMGTIEKGKDATLIVVSGDIMDMR
                     SSIVEQAFIQGRGIDLGNKQTDLYQRFKKKYDQN"
BASE COUNT         2259 a         1830 c         1640 g         2352 t
ORIGIN      
        1 gacatagact ccggcaatat aaacaaaatc ctgatctccg tcccggtcat aatcacacca
       61 agcgacactt gattccctgt tcgaatccgg aaggaatccg actctggaaa aagtgcgccc
      121 cgtacctcct tcatttctgt aaagaagccc cgcagctaat aaatcctgtt tgccgtcgaa
      181 atcataatct ccccatcgga ttggcataac aatgttcccc atggcaaccg gagaaagaga
      241 tgtgtcaaca gtaaatgcac tggtgccatt gcctttgtat atctttagcc tcggatcgcc
      301 tcctgatgtg ggcggatcat agttagaagt agatcccatt atggcaaaat ccaaaaaacc
      361 atcgttatta taatccccaa aatggacgcc gcccgcataa agattgtcga ttgagctcaa
      421 ttcatcagtg aatgaaccgt tttgattact gtaaagtttt gttgcaattg tagagccatt
      481 attgccggca acaagaatgt ccagatcgcc gtcattatcg taatcaccca aatcaagtgg
      541 acttcctaag ctaaacaatg ttgatcctac agagctttca gtaaatgaaa tacctgcgct
      601 ttcggtcgta tcgaatgccg cggcgcttcg cgtttcaccg aattcattaa ccgcggccac
      661 tttgaaataa tacgtagtcg ctgttccaag tcccgtcgcg ctataggaat ttgcggacgg
      721 actgctgatg gagtcccgtt gtacatagtt gttatcaatg ccatccgacc gatagatgcg
      781 gtacttcgtc gggccacctg tactggccgt ccatgagaga ttaatctgcg acgtagaaac
      841 ggccgtggcc gtcatatttg caggggccgc cggcgcgaga ttaaattcgg caggcgaacg
      901 tgcgatattg gaaatacgaa tttcgtctat ggagcctttg aaataataat ttgattcccc
      961 ccaaccaaaa atgagcggtc tggataagtt gggtacagta cccggagagc caatggatcc
     1021 ggacgcaacc ttgattccgt tgatatatat ttttacagag tcgcttttgg taacagccaa
     1081 atggtaccat tgatctgtaa cgggagtcca tacatactgc gtcgtagatc cgggatagac
     1141 aaattccagt ttattcgcac tgatctcata atgcaatctc cagtaatctg atccggaacc
     1201 aatttgcccg acaataattc catgagacgc attggaggaa aaatttaccc agcattcgat
     1261 ggtgaaggga tttgagccga agctccaatc ggcatgatcg ggtactacta tagaatcagt
     1321 aataaaactt cgtgcattat tgtatcgtcc tgatacgaca gtggggttcc cgccggtcgt
     1381 accgtggtgc cctctgccgc tcgcgtcggt gatcgttgta cccgatgcct catctaaccg
     1441 ccaaagcccc agcgtattcg catcagaact aattccctgg ccataagacg tattacccca
     1501 cgtcaaagca atggcggtta ccaagtagta aatcaagttt ttcataggct ctcgtttgat
     1561 tttgtttttc tattgcacga ccgcttttca cagataagtt taagagctaa tttttaagaa
     1621 atattttaac ctgttcttaa tcacaaatgc gttattgttt gggaggactt ggcggatttg
     1681 ggaatccact tgctgtggta ccgctcttgc caccgcttag taaaacggcc gccgctgcac
     1741 cgccggctac taccactccg ccaaggatat accatttggt tttagaaact ctatacgtac
     1801 cggtgatctt ctgcgccata acatcaaata ccgtaagcaa tccgtccggt ttaccgcggt
     1861 attcctcatt gatcgtcaca tccacttttc ctgttgtcac atctaaaagt ttgatcgtga
     1921 ccgaataagt ctctcctatt ttgccgatgt ctccagttac catcttctct gcggccaata
     1981 actggccgat ctccaccgcg cactcgctgg agttacacac atcggacatc ttaaaatcct
     2041 gctctttgag aatcttgtcc atctcaccgc gctcaagcac cgtgtatttg ccggtattgg
     2101 aaaacgagtt acgaaattta ctcgtcagcg cagcggcttc ttgcggtgtc aatcccgata
     2161 tgatcttaaa gtccaatact gcggccgtag gctttctttc tttctcttgt ttttgaccgt
     2221 atatacctat gccaaacact atcagcatga gtactactga ccaccctatt tgtctcatgt
     2281 cttctctccg tgtttttttt ctgaaatcgt tggtatgttt tttatatttg tcttgtcaca
     2341 atcacgcgat gaaataaagc ttccgattct gagggttaac ggcatgcatt cgcatcacag
     2401 ccaccatacg atgcacagca acaacaatcc ggaaatcgcg gcgagcataa tcataagacc
     2461 gaaatctctt aagaaattat ctttcacagc tactatctcc acttcgatga ttttattatt
     2521 tcgtattatt cttttcagaa atgccgccgt ttgggtcttt gattattttg tacacggttg
     2581 attttttcaa gcctgttttt tcaataatga ggcgtaccgg cataccgcga tgatgcattt
     2641 cgatcacatc acgcaccgct ttctgacgaa gtacatattt ttcagatggt acgtaagaac
     2701 ctctgaggcg cgtacatatt aggataacct tgtccaatac atgctggtgc ggttcggcga
     2761 taatttctgt aagtacgtct atgaattcac gtgtcgcatc gtatttgtcg ttttttgaca
     2821 tagccgtaaa gcccccttaa aaaacgaata tgagaaccaa aaataaggaa ttagacccga
     2881 aaggcgtgtg ctttaaattt agacgacaga atttccatct cccatagcac gcgtggaaat
     2941 tctgaataca cgcttagcaa aaaaattcgc ttgtacatcg catttcctat gtttaacttt
     3001 gtatactcat atagacaccg ttcttggtta tttcataact acattccctc atggattcag
     3061 accagctgtc gggcacattg ctcaaagcca ttgattttca attagataac gctgatgcgg
     3121 tgaacgatcg ttttgtgcaa tggcgtctga taggtacggc aaatgacggc gaatgggtgc
     3181 agacttgggt ttattgctgg acgagaagat atttttttcg caagtttctt tgcgggcaaa
     3241 ttcactctgc cggtgatacc gaaaaactga tcgaagaagt gtatttttta ttcattcaaa
     3301 aacacgctca tgtgcatact ccgtcgcatt ttgcggcatg gctttcggtc atctgcaagt
     3361 atcgctttct caactatatt cgttcttgcc atccggaaca ctggaacgaa gcgctgacag
     3421 aagtggtcgg caacgaagcc gataagtcta tagtacatga tttggacgtt aaactgtttt
     3481 ttgacaggat catctccggc gaaatgcgcc aacttccgga ctatgtacag acggttattc
     3541 acaaaaaaat atgggaagat aaagaatatc atgtgattgc tcaggagatg ggatacagtg
     3601 tggaaaccat acgcgcgtat tttgcgcgcg gcatcaaagc gcttcgcaat tcccatacaa
     3661 tccgcgaagc ctatgcagag agttatgaac cgaaccccta aattgtctat acagtaagag
     3721 attgtatgtt tgactctaac gaaaaactca gccgccagga aattgccgac tatgcgatgc
     3781 gcggtcattt tcctttgaca ccggaaaaag aagaatctat tcgccgacgc atagatatgg
     3841 atccggacgc tcagactgaa ctacaggtca tgttggagcg taaagcggga ttagatcgcc
     3901 taaccccctc ggaaaatcat tttgcacggc tccaaaagaa aaccacaccg tccataaaat
     3961 cacatttctc gaaaactcta tggttacgcg ctgcggccat ttttttgatc atagtcggga
     4021 gttatgccgg cttagtgact tatgtaaatt atcgtttcga tcacgggccg caaggcttgg
     4081 ctcagtttca aaaggattat gagtttgcca tagcgcccgc cgcgacccgc ggttccaatg
     4141 aaaaagagcc ggaggatatc cggctcttta aagaaggtgt gcgattgtta tttgaatcca
     4201 aagaaacgac cttcggtttg tctccgaggt atgatgaaga atcaatttct catgtcatcg
     4261 agattttcga aaaggtcgaa caaataaccg aaaacacaga cttgcgtaat agagcgtcat
     4321 taattgttga gaaatgtaag agcttcaatt agatcatttt ttttaaagaa gatccatttt
     4381 cttaacaaaa ccggaacctt caatttcaat ttcaatttta tcgctaatac cactatttaa
     4441 attattaaca tgtttttccc gataaaatcc gtaattagat gagtccctca tatttaactc
     4501 ttttgcaatc gtattgaagt cggtcatgac catatttcct tttgtgattc tcataatatg
     4561 atcggattgt attaaaacta tgtagtcacg tttggaactt gttgaattaa tattatcaat
     4621 caaaccttga aaatgattgc caggccatgc cgtcgtattc cgaaattcaa gagttggttt
     4681 gttaccttgt ttaaatcgca aaaacaacag cttgttgctt cctggtttat ctaaaagtaa
     4741 agcatcgcag gctaacgaca tttcaaaaag actgttttgt ggaatagcaa taattatttt
     4801 ttcagtccca atcgcgggtc tggcgcccga ttctacagtt agacagtttg gacaaggagg
     4861 agggggtaat ccaaccccgc tattgtcaac gcccaaaatt tgaactgtcc ccttcggcca
     4921 attgagataa attagccatg cggccataag aacgataatt gttaatgttc cgattttttt
     4981 cataaatctc tccgaagtta gtttaatgaa agtattagaa cacaataact atatattttt
     5041 tcttgtcggt tttcacttta ttgtaaatag tcttggtttt tctcaatcgg ctaccctatc
     5101 ggaaatcaat aaaatgattg acgggggcga cctttcacac gcatcaatat tattagacag
     5161 tctgctgcac caatccataa ctaaaaaaga ttccttaggg ataggttatg gatatctacg
     5221 tcttgggtta tgttatcaaa acctaaatga ctttgaaaaa gcaattgaat taaactcaca
     5281 tgccataccc attttcctgg gccttaagga tacgctgtac tatgcttggt cgcttgttac
     5341 agaagcgcat tatagcttga ttgcaaatcg atttcacttc gttttaagaa atctagaaaa
     5401 tgttttgaat ctgtgcaacg ctacacccaa actgaaatca atttattacg atgcgttgtt
     5461 tagaaccggt gcgttttatt tgaaaatcgg caattttgac caaagcataa aaactttgaa
     5521 aaaagctgaa aattattttg aatctaccta tgaccgtttt actctctcgg cggttctctt
     5581 taatatggga gcggcttaca aagaaacagg tgattataaa ctttctgaaa cctgtttaag
     5641 aagagtttta acccttgctg ccggcataaa gcaacccaat gatcgagcaa aggccgctta
     5701 ttcaatgcta tcagatcttt atcttagtat aggcaaatta gaccaggcaa tcgctatgga
     5761 gtatcttgct attaaggaac tcaaaacaga aaaagctgaa gccgatatac tcttttgcga
     5821 tcttggaaat ctttttctac gattgaagga tttccaaaag ggagaggatg cttttctaaa
     5881 aggcttgagt tatctcgcaa ccaattggaa tctacatagt gggcttgctt acattgccga
     5941 aattcgtggt aatattgccc aagctgattc ttttcatagc agggcaatta cattggcaaa
     6001 ccaacatata aaatatacac aaggcacggc aacaagcgca atacttaaaa aaagtattcc
     6061 aacctttaat caagccgccc gcttctattg gcgtatcgga aagccggaga aggccttcga
     6121 gttactcgaa accggccgcg ctcggtactt atcccaactt ttgaatttag ctaatgtcaa
     6181 atccgataaa tctgctttta ccgacagtat aagaaatatc gaaaagcaaa tcggggaact
     6241 ctaccggaaa ccggaatcgg gtttgagcac acatcaattg caaatcttcg ccctcgaggc
     6301 cgcgcgggag cagcttttgg attctataaa aaaatactct ccacagttct atgcctttaa
     6361 gaaaacaaaa attcccacac taaaggagtt gcagacttcg ctgggctccg aattccaact
     6421 catcgaatac gccgtaatgg acgattcgat ttttgctttg accgttaatt ctgaaaaagt
     6481 ctccggtcat gtgatgcaga tcagaagaga ttccctggag cgcatgatcg accgcgttat
     6541 cacggccaaa cagtcttcat tgcatgattt gcaacaattg tattggctac tcattacgcc
     6601 tatggaaaaa aatttgcaga ctggaaaacc gcttatcatc atacccgacg atgcgctctt
     6661 ccgcctgccg tttgaaatgc tcgtgaaaaa ccacgacggt aattaccgca gcgcaaaata
     6721 ccttttacat gactttccgg tgtcgtatgc agtttccgct tccgtatggt tggacaggct
     6781 tggacgtcat tcccgcgcgc caaaaaacta tgccgggttc gcggctgctg tatttgacac
     6841 gttatcagcc ctgccgtttg ttaaaaatca ggtcgacgat gcggcggaca ggttttctaa
     6901 ttccacttca tggactgacg ctgccgattt gcggcgactt tttgtgaatc gcgcaggaaa
     6961 atataagatc attgatatgg caacgcacgc tttcgtttcc gatatcgaac cgatgcgctc
     7021 caaaatcgtg ttcaacgaac acggcgggga gatgaatgcc ttcgagatat acgaactcga
     7081 tctcgacgcc gacctggccg tactcagcgc ttgcgggacg gggcttggta aactctctgc
     7141 cggcgaagga tttatgggct tcaatcaggc gttttcctat gccggcgtct acagtctgat
     7201 catgtccgcc tgggatattg atgacgaatc aacagccatg attatgaaat cattttacga
     7261 aggcattgca tccgggctta cccgcgcgga agcgctacgg cgagcgaaac taaaatatat
     7321 caacgaggcg tatgaactga aacaatcgcc ttattactgg gcggggctca tgctttgggg
     7381 caaccctgaa gcaattccct tgcaccgtgt gccgtatgga tttatcgctt ttttgttcat
     7441 tcctttcgcg attattatcg tatccccttt cgtcggaaga ttacgaaaaa agaaaaaaca
     7501 ctgacaaaca atttcgaaat catctgatag ctcaatgttt aataatttcg cttgtttgta
     7561 tctgtatcct aaagctcatg gccatattca acaattcaaa tcctattttc atgtgaatga
     7621 catccctggt atttgccccc tgcttcgctc tgtgaagcaa tatgaattgc cttatgaaaa
     7681 agccatcccc gtcaatggat aaaattaatt atgacattat caacttgata acgtggcttt
     7741 taaaaccaat ttgaataaaa tattacaagc cgagtttttt ataaaaaaag cgggctttgg
     7801 cccgctttcg atgattaaaa tgatcacttg ttatcaagct acttagtttt gatcatactt
     7861 tttcttaaac cgttgataca gatcggtttg tttattacca agatcaatac ctcggccttg
     7921 tataaatgcc tgctctacta tagatgaacg catgtccata atatcaccgc ttaccacgat
     7981 caaggtcgca tctttacctt tttctatagt acccatacga tcatttatac ctaaaatttc
     8041 agccggtgat ttagtaatag ccgtcaacgc ttcttcttta g
//
LOCUS       DAOQGF010000130         7990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4304_length_7990_cov_5.864650, whole genome shotgun sequence.
ACCESSION   DAOQGF010000130 DAOQGF010000000
VERSION     DAOQGF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_7990_cov_5.864650"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2661)
                     /locus_tag="PK937_13665"
     CDS             complement(<1..2661)
                     /locus_tag="PK937_13665"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNH33669.1"
                     /translation="MKRWLLLWVSTVFVSVTAQQFTKITTGPAVSDGGASRSVNWFDY
                     NNDGYLDLFVSNSSGAKGTSDFLYRNLGVSGNYGFSKIIRGPVVTDFGQSDGASFGDY
                     DNDGDEDVFVVNWYNENNMFYENGGAPYFRYSAVTTGAPVTDGGLSETCVWGDYNNDG
                     YLDLYVTNSGNAGLAQANFLYKNNGNKTFTKITTGSPVTDVAFTRGANWIDYDRDGDQ
                     DLFVVNESGQDNALYKNTLIENGTEGFLKVTGVSIASDGGSSMSASWGDLNNDGNLDV
                     LVVNGIGEHEFLYLQNNDHTFTKVTTGDLVNSGGSSMGSALGDFDNDGDLDVYITNSY
                     AGTPSVDFLFKNMFIENGSLSFQKITTGSIVTDQGDGYGCAWGDYDRDGDLDLFVAKT
                     AGEDQNNALYRNDNTTGYHSVQFRCVGTMSNRSAIGTIIRVKAVIGGQGRWQTRVVEG
                     QSGYCGQSQIAHFGLHNATTIDSVEITWPRGDVDIYTGLSSDSIYVLTENTGYGTLGF
                     SYNTEPIQSQTLRDTTVLEDFGRISFISLTDYFYDPDYTDLKFSVSAITSGITVSLTG
                     DSLICNSVKDFFGSVKIAVRATDDVVTLYDTLTITIEDVAEPILRPAVTVSVFQNPVL
                     SSYADIIVVSTLDLDTAPSLQIQIPGGSGDVSLSLQAISESARIFKTSYKLQTNGTYT
                     LSAGIRTYQLFDTTISRSFTASLAKVGQTQIAFSPDERSHLTVPAGQSYGLDWILATI
                     EETADEIVYNYQAKSLPPYTRFEYVADMSQMPAGMRPVISRWDGVQWVVRPSIYNERT
                     HIVSTEISEAGQFKILFVNDDTPSAIIPEDLQLEQNYPNPFNPSTHIRFAVPQASSVR
                     LTVFNLLGQKVRTLIEDYRSSGSYGVVWDGR"
     gene            complement(2806..3540)
                     /locus_tag="PK937_13670"
     CDS             complement(2806..3540)
                     /locus_tag="PK937_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNH33670.1"
                     /translation="MRVLIVDDEMPGRQAIKDLLRNESGMHIIGEAGDGHEAVRQITE
                     QKPDLVFLDIQMPGLDGFGVVRQIGIERMPMTVFVTAYSMHALNAFDVHAMDYVLKPI
                     HPERFAACLKRIRQLKTPNVPAMVGAMTMVQQKYPSQILIRSTGKMDVIKTDDIRWVE
                     AQGDYVQIYTRSQKQLMRSTMSGFETLLDPSVFQRIHRSVIVRVSLIKELQPMFNGDY
                     KIKLDDGTSLSLSRTYHEKVLHCLNG"
     gene            complement(3546..4634)
                     /locus_tag="PK937_13675"
     CDS             complement(3546..4634)
                     /locus_tag="PK937_13675"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNH33671.1"
                     /translation="MNKPDRKFWIIVAAAWLLLGLLYSIQSYHYRVTIGRPGDLLPLL
                     INDIGFFFLWALFSVPVIRWILHWPILRSNWQRRLPVFLMAGLVIAFSQKGLYDAFLL
                     VAYGETTQGFPWERWYRSVIGSFELGFFIYVSMIFVVHTWIYHVNLQREQLDAAALRE
                     RLSQIRLQVLQSQLQPHFLFNTLNTISGLIDVRPADAKLTLGRLGELLRMSLESDQLT
                     EVTLQRELDFSQVYMAIQTTRFGDRLTYKCTVDSAMYGVLVPTMMLQPLLENAVTHGV
                     SKVPGVHMIELKSWRDGGMLCLDINNSFEAIAKPEQAPGLGIGLTNIRERLRQLYGEM
                     AKCELAVHAHGAYVTIRMPWRTSDGNKG"
     gene            complement(4854..6725)
                     /gene="thiC"
                     /locus_tag="PK937_13680"
     CDS             complement(4854..6725)
                     /gene="thiC"
                     /locus_tag="PK937_13680"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNH33672.1"
                     /translation="MSNNNVKIPNAQSKITRDPFPASKKIYVEGSLPGVYVPMREIQI
                     NSRGAHGVKSNGSSTEQVTVYDTSGPYTDPDAQVDIYKGLAGVRSPWIQTREVQALEG
                     ISSQYGRQRENDPSLDAIRFKRTRKVLKAKPGSNVSQMHYAKKGIITPEMEYIAIREN
                     QKRESAIAEYAKQHPGEHFGASIPPVITPEFVRSEVARGRAIIPANINHPEIEPMIIG
                     RNFLVKINANIGNSAVASSIEEEVEKMIWAIRWGADTVMDLSTGKNIHETREWILRNA
                     PVPIGTVPIYQALEKVGGKAEELTWEIYRDTLIEQAEQGVDYFTIHAGVLLRYVPLTA
                     KRVTGIVSRGGSILAKWCLAHHKENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSIAD
                     ANDDAQFGELETLGELTKIAWKHDVQTMIEGPGHIPMHMVQVNMTKQLEVCGEAPFYT
                     LGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPNRDDVKDGVITYKI
                     AAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPDTAREFHDETLPAEGAKV
                     AHFCSMCGPHFCSMKITQDVRDYAESIKTDETKALEVGMREKAEEFKKTGSKIYVEV"
     gene            6854..7528
                     /locus_tag="PK937_13685"
     CDS             6854..7528
                     /locus_tag="PK937_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNH33673.1"
                     /translation="MAHVTMHTSGEKIPVSKILCVGQNYREHIIELTPEGKEPDFPTE
                     PVIFMKPPSALIYDGGTILRPSVSNDMHHEVELIIVIGKTGKQISPADAMHHVLGYAV
                     GLDMTLRDLQSEAKKKGRPWTIAKGFDTSACISQVMPKEQTGNIMQQDIKLLVNGTIR
                     QNGNTSDMIFDIATIVSYVSQFFTLERGDLIYTGTPKGVGPVVTGDQLHAILGNDLLK
                     VNAKVG"
     gene            complement(7683..>7990)
                     /locus_tag="PK937_13690"
     CDS             complement(7683..>7990)
                     /locus_tag="PK937_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNH33674.1"
                     /translation="AEHSTAILVSAKNQMDLAMNIAIGSSIQIALFVAPVLVFGSYFF
                     GQPMDLIFTTFEVLAIALSVAVLALVTMDGESNWMEGVQLLAVYVILGIAFYYLPAH"
BASE COUNT         2156 a         2034 c         1743 g         2057 t
ORIGIN      
        1 acgcccgtcc cagacaaccc cgtacgaacc ggatgaacgg tagtcttcga tgagtgttct
       61 cactttttgg cccagcaaat taaatacggt aaggcgtacg ctcgacgcct gcggaacagc
      121 aaatcggata tgcgtagaag ggttaaatgg attcggataa ttttgttcaa gctgcaaatc
      181 ttccggtatg atagccgatg gggtatcgtc gtttacaaat aaaattttga attgacctgc
      241 ctcggaaatt tctgttgaaa cgatatgtgt gcgttcatta tagatcgaag gccgaactac
      301 ccattgaaca ccgtcccaac gtgaaatcac cggacgcatt cccgccggca tttggctcat
      361 atcagcaaca tattcaaatc gggtatacgg cggcaatgat tttgcctgat agttatatac
      421 tatttcgtcg gccgtttctt cgatcgtagc caaaatccaa tccaaaccat aggattgccc
      481 cgcgggtaca gtcagatgtg aacgttcgtc cggtgaaaat gcaatctgag tttggccgac
      541 ttttgcaagc gatgctgtaa agcttcggct aatcgtagta tcaaacaact gataggtacg
      601 aatacccgcc gataacgtgt acgtaccgtt ggtctgcagt ttatacgatg ttttgaaaat
      661 acgcgccgac tccgaaatag cctgcaatga aagcgatacg tcgccggatc cgccgggtat
      721 ctgtatttga agcgatggtg cggtatccag atctagtgtg ctcaccacga taatatccgc
      781 atacgatgac agtaccggat tctgaaatac ggacactgtc acagccggtc ggagtatggg
      841 ttcggcgaca tcctcgattg ttatggtcag cgtgtcatag agagttacga catcgtcggt
      901 agcacgtacg gcgattttga ccgaaccgaa aaaatctttt acgctattac atatcaggct
      961 gtcgccggtt aggctcaccg taatccccga tgtaatcgcg gataccgaaa acttgagatc
     1021 ggtataatcg ggatcgtaaa aataatccgt gagtgaaata aaagatatac gcccaaagtc
     1081 ttctaacacc gtagtatcac gaagcgtttg cgattgaatc ggttccgtat tataagaaaa
     1141 gcctaacgtc ccataaccgg tattttccgt caacacgtaa atactatccg aagacaagcc
     1201 ggtataaata tccacatccc cgcgcggcca ggtaatttct accgaatcaa ttgtcgtcgc
     1261 attatgcaat ccaaaatggg ctatctgtga ctgcccgcaa taaccgctct gaccttcgac
     1321 gacgcgtgtt tgccaccggc cttgaccacc gataaccgct tttacgcgga tgatagttcc
     1381 tattgcactg cgattggaca tcgtgccaac acatcgaaat tgtacgctgt gataaccggt
     1441 cgtattatca ttgcggtaca acgcgttatt ctgatcttcg cccgctgttt tggcaacaaa
     1501 caaatccaaa tcaccgtctc tatcataatc gccccatgcg caaccgtagc cgtcaccctg
     1561 atccgttacg atactgcctg tcgtaatttt ttgaaaagaa agggaaccgt tttcgataaa
     1621 catgtttttg aataaaaaat ctacggatgg cgttccggcg taggaattcg taatatacac
     1681 atcaagatcg ccgtcattgt caaagtcgcc aagcgcactg cccatagatg aaccaccact
     1741 gtttacgaga tcaccggttg tgactttggt gaacgtatga tcgttattct gaagatacaa
     1801 aaactcatgt tctccgatgc cgttgacaac taacacgtca aggtttccat cattgtttaa
     1861 atctccccag ctggcgctca tggaacttcc cccatctgaa gcaatagaca ctcctgtcac
     1921 tttcaaaaaa ccttccgtac cattttcaat caacgtattt ttatacagcg cgttatcctg
     1981 cccgctctca ttaactacaa ataaatcctg atctccgtcc cggtcataat caatccagtt
     2041 agcgccccga gtaaatgcga catctgtaac cggtgaacct gtcgtgattt ttgtaaatgt
     2101 tttattaccg ttattcttgt acagaaaatt agcctgcgcg agaccggcat tgccgctgtt
     2161 ggttacgtac aaatccagat acccgtcatt attatagtcg ccccatacac atgtctccga
     2221 taagccgcca tcggtcaccg gagcgccggt cgtaacagct gaatagcgga aatacggagc
     2281 gcccccgttt tcataaaaca tattattttc attgtaccag ttgaccacaa aaacatcttc
     2341 atcaccgtcg ttgtcatagt cgccgaaact cgccccgtcg gattgcccga agtctgtaac
     2401 caccggacca cggataattt tactgaagcc gtaattgcct gaaacaccga gattgcgata
     2461 caaaaaatcc gatgttccct tagcgccgct gctgttactg acaaacaagt ccaggtaccc
     2521 gtcattgtta taatcaaacc agtttacact gcgcgaagcg ccgccgtcgc taacggccgg
     2581 cccggtagta attttggtaa attgctgagc cgtgaccgat acaaatactg ttgaaaccca
     2641 tagtaacaac catcgtttca tacgcgtctc ctattatatc tattcatggc caacatacgc
     2701 gatgaaacgg cgccgtccta tccaaacgcg ataatacgtt agaaaaacag gttgaaccgt
     2761 gaggattatg cgatgagaaa ctgaaaggtc agattggttc ggtgatcaac cgttgaggca
     2821 gtgtaatact ttttcgtgat aagtacggct tagagaaagt gacgtaccgt cgtctaattt
     2881 gattttataa tcaccgttaa acatcggttg gagctctttg atcagactga cccgaacaat
     2941 gacggatcgg tggatacgtt gaaaaacgga agggtcgaga agcgtttcaa atccactcat
     3001 cgtgcttcgc atcagttgtt tttgcgatcg ggtatagatt tgcacatagt caccctgcgc
     3061 ttccacccag cgaatatcgt cagtttttat cacatccatt tttccggtgg agcgaatgag
     3121 tatttgtgaa ggatactttt gttgaaccat cgtcatcgca ccgaccatgg ccggtacatt
     3181 cggcgttttc agctggcgaa tgcgtttcaa acacgccgca aaccgttccg gatggatagg
     3241 tttgagcacg tagtccatag catgcacatc aaaggcatta agtgcatgca tgctgtaagc
     3301 cgtcacaaat accgtcatcg gcatgcgttc gataccgatc tggcgtacaa caccaaatcc
     3361 gtccaaaccg ggcatttgaa tgtccaaaaa aacaagatcc ggtttttgct cggttatctg
     3421 acgcaccgct tcatggccgt cgcccgcttc tccgataatg tgcatgcccg attcgttacg
     3481 caggagatct ttgatggctt gacgcccggg catctcgtca tctacgatga gtacgcgcat
     3541 agatgttatc ctttgttccc atcggaggtg cgccacggca tcctgatcgt aacgtaggca
     3601 ccatgtgcat gtaccgccag ttcacactta gccatctcac cgtacaattg ccggagacgt
     3661 tcacggatat tggtaagacc gatgcccagt cccggtgctt gttccggttt ggcaatagcc
     3721 tcaaagctat tatttatatc caaacacaac atgcctccat cacgccatga tttcaactca
     3781 atcatatgga ctccgggaac ttttgaaaca ccgtgcgtca cggcattttc cagcaaaggt
     3841 tgtaacatca tcgtcggcac caacacccca tacatcgcgg aatcgactgt acatttatat
     3901 gtcagacgat cgccgaaacg ggttgtttga atagccatat acacttgcga aaaatctaat
     3961 tcacgttgaa gtgttacttc ggttaactga tccgattcca gcgacatacg aagcaattcc
     4021 cccaatcggc caagcgtcag ttttgcatcc gcaggacgaa cgtcaataag tccggaaatc
     4081 gtatttagcg tattaaaaag aaaatgcggc tgcaattgac tttgtaaaac ctgcaaacgg
     4141 atctgtgata atcgttcgcg caaagcggcg gcatccagtt gctcacgctg taggttgaca
     4201 tgataaatcc acgtatgcac aacaaagatc atggatacgt aaatgaaaaa accgagttca
     4261 aaagaaccga tcaccgagcg gtaccaacgt tcccaaggaa aaccctgtgt cgtttcgccg
     4321 taagcaacca gcaaaaaagc atcatacaat cccttttgag aaaaggcgat gactaaaccg
     4381 gccattaaaa aaacgggtag ccgacgctgc cagttgctcc gaagaatcgg ccagtgcaaa
     4441 atccatcgaa tgacgggaac gctaaacaac gcccatagaa aaaaaaagcc tatatcgttg
     4501 ataagtaacg gtagcaaatc accggggcga ccgatggtga cgcgataatg gtaggattgt
     4561 atgctgtaga gcaaacccaa aagcaaccaa gccgcggcta ctatgatcca gaatttgcga
     4621 tcgggtttat tcatgggtat taaaatgatc gtgacgactt ttggaataat gccggtataa
     4681 attaacgacc gttagagatt ttgcaacacg tgttgttata ctcttaaatc tcaacagcat
     4741 gcctaggaac tcggtttaaa ctacaaaatt tataccgtga ttacttcacg cccaacgtat
     4801 cctttcatac gcattttaga agccgccgtt ttttattcag gccgctagcc gtgttatact
     4861 tcaacgtata ttttggatcc ggtttttttg aattcttcag ctttttcacg cataccgact
     4921 tctagcgctt tggtttcatc cgtcttaatg gattcagcat aatcgcgtac gtcctgagtg
     4981 attttcatgc tgcaaaaatg cgggccgcac atactgcaaa aatgcgccac tttggcacct
     5041 tccgccggca acgtctcatc atgaaattcc cgcgcggtat ccggatccag cgacagatta
     5101 aactgatctt cccaacgaaa ttcaaaccgc gctttcgaca gcgcgttatc gcgaatttgc
     5161 gctccgggat ggcctttggc caaatcggcg gcatgcgcag cgattttata agtgatcacg
     5221 ccgtctttga cgtcatcgcg gttgggcaat cccaaatgtt ccttgggtgt gacgtaacac
     5281 agcatggcgc aaccgtacca tccgatcatc gctgcaccga tacccgacgt gatatgatcg
     5341 taacccggcg cgatgtccgt cgtcagcggc cccagcgtgt aaaacggcgc ttcaccgcac
     5401 acttcaagtt gtttggtcat gttgacctgc accatgtgca tcgggatatg gcccggccct
     5461 tcgatcattg tctgcacatc atgtttccat gcgattttcg taagctcgcc aagcgtctcc
     5521 aattccccga actgcgcatc gtcattggca tcggcgatgg aacccggacg caaaccgtca
     5581 cccaaactaa aactcacgtc ataggccttc atgatctcac agatttcttc gaaatgcgtg
     5641 tacaaaaaat tttctttatg atgcgcgaga caccacttag ccaatatcga accgccgcgt
     5701 gacacgatac ccgtcacacg tttggctgtg aggggtacat accgcaataa aacaccggca
     5761 tgaatcgtaa aataatccac gccttgttcg gcttgttcga tcagcgtgtc gcggtaaatc
     5821 tcccaggtaa gttcctccgc tttaccgccg actttttcga gcgcctgata gatcggcacg
     5881 gtaccgatag gtacgggtgc attccggagt atccattcgc gcgtttcatg tatatttttt
     5941 ccggtggaaa gatccatcac cgtatccgca ccccaacgga tcgcccaaat cattttttcc
     6001 acttcttctt caatgctcga ggccacagcc gaattaccga tattggcatt gatcttgacc
     6061 aaaaaattac gacctatgat cataggttcg atctcggggt gattgatatt ggcaggaata
     6121 atcgcccgtc cgcgcgctac ttcgctgcgc acaaactccg gagtgatcac cggcgggatc
     6181 gaagccccaa aatgttcgcc gggatgttgt ttcgcatatt ccgctatcgc cgactcccgt
     6241 ttttgatttt cacgaatagc gatgtattcc atttccggag tgatgatgcc ttttttcgcg
     6301 taatgcattt gcgatacatt gcttccgggt ttggctttga gcactttgcg tgtacgtttg
     6361 aaacgaatgg catccagcga tggatcgttt tcacgttgac gcccgtactg tgaggaaatc
     6421 ccctccaatg cctgcacttc gcgcgtctgt atccacgggg aacgcacgcc ggccaaacct
     6481 ttatagatat ccacctgtgc atccggatcg gtgtacggtc cgctggtatc atatacggtc
     6541 acctgttcgg ttgaactccc gttactcttc acaccatgcg cgccgcggct attgatctgt
     6601 atttcgcgca tcggcacgta cacacccggt aaacttcctt cgacatagat ttttttggaa
     6661 gccggaaatg gatcgcgtgt tattttgctc tgggcgttgg ggattttgac attgttattg
     6721 gacataagtt ctcctgtatg tacggaaaat tttttcaata tttagacaga cggtgcgatt
     6781 agaaattatc tcttcctgat cgcgcaaaaa cttactaagt tattgcacac aattcaagcc
     6841 caaggataat atcatggcac atgtcactat gcacacatcg ggagaaaaaa tacccgtttc
     6901 aaaaatttta tgcgtcggcc aaaactaccg cgaacacatc atcgaattga cgccggaggg
     6961 caaagagccc gattttccta ccgaaccggt gatttttatg aaaccgcctt ccgcactgat
     7021 atacgacggc ggtacgatcc tacgtccgtc cgtttccaac gatatgcacc atgaggtcga
     7081 actcatcatc gtgatcggaa aaaccggaaa acaaatatct ccggccgatg caatgcatca
     7141 cgtactcggt tacgctgtcg gactagacat gacattgcgc gatctgcaaa gcgaagccaa
     7201 gaaaaaagga cggccctgga cgatcgctaa aggctttgac acctcggcgt gcatttctca
     7261 ggttatgcct aaagaacaga ccggaaatat catgcagcaa gatatcaaac ttcttgtcaa
     7321 cggtacgatc cgtcaaaacg gcaatacatc ggatatgata tttgatatcg cgactatcgt
     7381 aagttacgtt tcacaatttt ttacactgga gcgcggcgat ctgatctata cgggaacacc
     7441 taaaggcgtc ggcccggtcg taacaggtga tcaacttcac gccatactgg gaaatgattt
     7501 gttgaaggta aatgcaaagg tgggatagtc tatcgcagaa taatttttca gaaacgaacg
     7561 ttacgtattc ccgtcaaaat gactgacaac tgttttaccg gcgatgcgta aaaaaataag
     7621 gacgcctcgg catgttgtgt taaacacaac cctcgacgtc ctgcaaaact attctaagct
     7681 ttttaatgcg cgggcaaata ataaaatgcg atacctaata tcacgtacac cgccaaaagc
     7741 tgaacgcctt ccatccagtt ggactcaccg tccatggtca ctaaagccaa cacagctacg
     7801 gacagcgcga tagcaagaac ttcgaatgtc gtaaaaatca aatccatcgg ttgaccgaaa
     7861 aaataactcc caaacaccag taccggcgca acgaataagg caatctggat gcttgacccg
     7921 atagcaatat tcattgcaag atccatttga tttttagccg acactaaaat agccgtactg
     7981 tgttctgccg
//
LOCUS       DAOQGF010000131         7940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4344_length_7940_cov_8.377933, whole genome shotgun sequence.
ACCESSION   DAOQGF010000131 DAOQGF010000000
VERSION     DAOQGF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_7940_cov_8.377933"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK937_13695"
     CDS             complement(<1..402)
                     /locus_tag="PK937_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH33675.1"
                     /translation="MKRVTGLGGVFFRCEDPGSTMAWYQKHLGVQPDGYGGSFFEWLE
                     KDNTEEIGITAFSTMSKKTKYFHPSRAQFMINFRVKNLKKLMEVLRAEGVQQIGKIET
                     FEYGSFGWILDPNGVKIELWEPVKGYRFKRKR"
     gene            685..1332
                     /locus_tag="PK937_13700"
     CDS             685..1332
                     /locus_tag="PK937_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH33676.1"
                     /translation="MPEDFEIRTRIVKAALELFVKFGYSKVTMDEIAENLGMSKKTLY
                     QYFPSKQELIQGIIQSAMHEYDTFCDRIIGDQTMRFEDKLRQMLTYFLRVNGNLSREL
                     VADLRKHLPDMWQQIHEWRHTRIAAQFGLVLREGISKGSIRNDIDEKLIIMMYLGIIE
                     NMMNPQVIMQLSQSAPQIFESIWKVFFEGILTADARKTFSVSATVALPTITPQGV"
     gene            1336..2697
                     /locus_tag="PK937_13705"
     CDS             1336..2697
                     /locus_tag="PK937_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH33677.1"
                     /translation="MKSNRIFKIVLILSFLFSLKIIFAQSQRALTEEEAVKMAVETSK
                     QLRIVEMKAQGASARAKEMRTYRLPSAKLTAGYTRLSEVPDGEFSIPANAFGPGIPSR
                     DIVSKVAPAILNSYLLKGTIQYNVFTGMKLESNEKAARLSEEAAVLDFSKEKKDIVFN
                     TRTAYWNLFKAIEYNKVVDENLEQVKAHLKDAQNLYAQGMITNNDVLKVQVQLSNVQL
                     LQIDAKNAVKLAMVHLNNLIGQSLDTEIQIVSKIQNTDRTFSNYDSLLYKGWTNREDV
                     KAMELRIQAGEASVRAAKGSYMPQVSLQGNVYYNRPNQRIFPSEDAFKYTWDVGLNAT
                     FDLWNWNATAHQVQQAKAVVEQTKDALGLIKDGISLEVTQSYLAMQQAKEKISVTRQT
                     VEQSEESYRITSEKFKKGVALTTDLLDAEVSVLQAKTAYTNAIVDFELAQARLQKAIG
                     QNE"
     gene            2735..3787
                     /locus_tag="PK937_13710"
     CDS             2735..3787
                     /locus_tag="PK937_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH33678.1"
                     /translation="MRKLKITLGIFAVIAFIALGLWNNKVRSASKMNASIAITSVSVS
                     VAPAVMEKIDQAISVVGTITANNDVAIVSETQGRVVSVNAKVGDQLGAGSVIAQVDDE
                     LKKANYLLAEANYQKTKKDLERNEELFKNNAISTSQIESSRLAFQSAEAQYIVARRQY
                     NDTRITTPIGGIVSSRSVDFGTMVVPGTVIANVVDVSKLKVKIQLAEHDVFKIKNGDK
                     AVVSTDVYPGMEFEARVENISAKADEAHTYPVELSLNNNSKNPLKAGMFARVDFKTFV
                     ERTSVVIAREALVGSLKKPQVFVVEGDKAVLRNIVVGVTSSHKIEVLSGLKEGENVVI
                     KGQNNLQDQSTIRVAQ"
     gene            3844..7029
                     /locus_tag="PK937_13715"
     CDS             3844..7029
                     /locus_tag="PK937_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH33679.1"
                     /translation="MSITELAIKRPSLIVVIFGALTVLGIFGYNQLSYELLPKFAPPV
                     VTISTIYPGASPSEVENSVSKPIEEAVSGLDNVSRINSTSQEGFSLVFVRLTQSANID
                     LALQEAQRKVNGILATMPRDVKTPVITKFALDEFPVLRMGATSSMGPREFYQFLKDRV
                     VPRIAKQSGVAQVTLVGGNEREIKINLDAQKIKSYGLSLARVAQTIKASNLDFPTGNI
                     KQEGTQFVVRVAGKFESVEALSLLVVGRSKQGGDIKLSDVAEVHDGQKDMSTLSRING
                     NESIGILVQKQSDANAVRVSELVRKELASMEKDYENVKLKITIAQDGSQFTVDAANAV
                     KFDLMLAIVLVALTMFLFLHSIRNSIIVMIAIPASLVSTFFAMYLLGFTLNLMTLLAL
                     SLVVGILVDDSIVVLENIYHYLEKGLKPRDAAITGRNEIGFAALSITLVDVVVFVPLA
                     MTTGLIGNIMRQFAIVVTVSTMMSLFVSFTVTPLLASRFSKLERMTNGTFLGRFALWF
                     EALYEAFKNHYLRLLRWALSHRKTVVTATVLLFFSSCTLVPFGFIGNEFVTQGDRGEF
                     AVFLELPSGATIESTNHATQQAEKIITAMPEVSKVFTNVGASSDGFLTMSSNNISELN
                     VTLVDKSERTKTTDEVGALIKARIAEIPGVKVRVNPIGIFGTANQTPIQIVVSATNEA
                     DMKHAADRAMQILAAVPGATDVKLSAEDGKPETRIEVDRQKMAAFGLTIADIGQTLRI
                     ALTGDEDSKFRDGANEYTIRIRLDEFDRSNLNDVRHLSFINSRNEQIELQQFASIYQT
                     AGPSKLQRQDRFGSITVMGQVLGRPSGNVVQDFQKAIALEKDKLGKDVTFTYLGDEEN
                     RSEGFGSMGLAFLAGVLFVYLIMVALYDSYLYPFVVLFSIPVAGVGALLALALAIKSL
                     SIFSLLGMIMMIGLVAKNAILLVDRTNDMKAQGMQTYDALMEAGSARLRPILMTTMAM
                     VIGMLPIALASGAGAEWKTGLAWALIGGLTSSMFLTLIFVPIVYLKFDDWKNRVPQFF
                     KRLTGKKNEPVILATGSNGHYGQAETVKSEA"
     gene            7376..7588
                     /locus_tag="PK937_13720"
     CDS             7376..7588
                     /locus_tag="PK937_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33680.1"
                     /translation="MDKFIRRLIPQAVGITCMVIGWWISIVNVGLDKFVSSTIVNKWT
                     MIGLLLIIIGAYIPQIWIFILNKLNK"
     gene            7732..>7940
                     /locus_tag="PK937_13725"
     CDS             7732..>7940
                     /locus_tag="PK937_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33681.1"
                     /translation="MCCISISAPFLNAQENDSLQTRIKKNFLVIPALFYTPETKLGYG
                     AIAAIYFRKPFPENAKPTTLVPSVI"
BASE COUNT         2210 a         1840 c         1786 g         2104 t
ORIGIN      
        1 tcgtttgcgt ttaaagcgat accctttgac cggttcccac aattcgattt ttacgccatt
       61 ggggtcgagt atccagccaa aagaaccgta ttcaaaagtt tcgatcttac cgatttgctg
      121 aactccttcc gcccgtaata cttccatcag tttcttgaga ttcttcacgc gaaaattgat
      181 cataaactga gcgcggctcg gatgaaaata ttttgttttt ttactcatcg tgctaaacgc
      241 cgtgatgccg atctcctccg tattatcttt ttccagccac tcaaaaaaac tcccgccgta
      301 tccgtcgggt tgaaccccga gatgcttttg ataccaagcc atggtcgaac ccggatcttc
      361 gcaacgaaaa aaaactccgc ctaaaccggt aacgcgtttc ataaaaacct ccttgtatat
      421 gggagtgggc tataatggta cttcgaaagt atcacgtacg atacccaagt tcaacaaaaa
      481 tgaaagaatt acttgacaaa agtcactttt tttgtatatt ggaaaccaag aaaacaaata
      541 tagttttctc gaaaaatgat taagtaattg atttagctag attaaacatc ggctaatttt
      601 tttgaaaaaa tggaaactaa tgaaactttt ttagtttcca cacgtttgaa tgggtcatta
      661 aaatttattt taaggagtcc aaaagtgccc gaagattttg aaatcagaac ccgcatcgta
      721 aaagccgctt tggagctttt tgtcaaattt ggttacagca aagtcactat ggacgaaatc
      781 gctgaaaacc tcggcatgag caaaaaaacc ttgtaccagt atttcccaag taaacaagag
      841 ttgatacagg gcattataca gtcggccatg cacgaatacg atacgttttg tgatcgtatc
      901 atcggcgatc aaacaatgcg ttttgaagat aagttgagac aaatgctgac gtattttttg
      961 cgtgtcaacg gtaaccttag ccgtgaactc gtggccgatc tgcgcaaaca cttgcccgac
     1021 atgtggcaac agatacacga atggcgccac acgcggatcg cggcacagtt tggtcttgtt
     1081 ttgcgtgaag gcatcagtaa aggttccata cgcaacgata tcgatgaaaa actgatcatc
     1141 atgatgtacc tcggcattat cgaaaacatg atgaatccgc aagttatcat gcagttgtct
     1201 cagtcggcgc cgcagatttt tgaaagtata tggaaagtat ttttcgaagg catccttacc
     1261 gccgacgccc gtaaaacatt ttccgtcagc gccaccgtcg ctttacccac gattacgcca
     1321 caaggagttt gatatatgaa aagcaaccgg atattcaaga tcgtgctcat tttgagtttt
     1381 ctttttagcc taaaaattat ttttgcccaa tcgcaacgcg ccctgaccga agaagaggcc
     1441 gtaaaaatgg cggttgaaac cagcaaacaa ctgcgcatcg tcgaaatgaa agcgcaaggc
     1501 gcttcggccc gcgcgaaaga aatgcgtacc taccgcttac cgtctgccaa actgacggcc
     1561 ggatatacgc gtttgagtga agtaccggac ggcgaatttt caattccggc taatgctttt
     1621 ggccccggta taccgtccag agatatcgtt tccaaagtag cgccggctat tcttaacagc
     1681 tacctcctca aaggtacaat acaatacaat gtattcacgg gcatgaaact cgaatccaat
     1741 gaaaaggctg cacgcctctc cgaagaagcg gcggttttgg attttagcaa agagaaaaaa
     1801 gatatcgtat ttaatacgcg cacggcgtat tggaatctgt ttaaagcgat cgaatacaac
     1861 aaagtcgtgg atgaaaacct cgaacaggtc aaagcccatc ttaaagatgc ccaaaatctt
     1921 tatgcgcaag gtatgatcac caataacgat gtgctcaaag tgcaggtaca gctttccaat
     1981 gttcagcttt tacagattga tgccaaaaat gcggtcaagc tggcgatggt tcatctcaat
     2041 aatctgatcg gccaatcgct cgacacagag atccaaatcg tttccaaaat acaaaacacg
     2101 gatcgtacct tttcaaacta tgattccctg ctctataaag gttggaccaa ccgtgaagat
     2161 gtaaaagcta tggaattgcg tattcaagcc ggtgaagcgt ccgtccgtgc agccaaaggt
     2221 agctacatgc cgcaggtatc gctgcaaggc aacgtgtatt ataaccgacc caatcaacgt
     2281 atttttccct ctgaagatgc ttttaagtac acctgggatg tgggtctgaa tgccactttt
     2341 gatttgtgga actggaatgc gacggcacat caggtgcagc aagccaaagc ggtcgttgaa
     2401 caaaccaaag atgcgctcgg ccttatcaaa gacggtatca gtctggaagt gacccaaagc
     2461 tatctggcga tgcaacaagc taaagaaaaa atttccgtta cccgtcagac ggtagagcag
     2521 tcggaagaaa gctatcgtat tacttccgaa aaatttaaaa aaggcgtcgc tctgaccacc
     2581 gatttattag atgccgaagt ttccgtgctt caagccaaaa cggcttatac caatgcgatc
     2641 gtggattttg aattggctca agcgcgtctg caaaaagcca tcggacaaaa cgaataattt
     2701 ttaaataaaa aactataaaa tggagtttta tatcatgcgt aaactaaaaa taacactcgg
     2761 gatttttgcg gtcatagctt ttattgcgct cggtttgtgg aataacaaag tgcgcagcgc
     2821 ttccaaaatg aatgcttcga tcgcgatcac atcggtttcc gtatccgtcg caccggcggt
     2881 catggaaaaa atcgatcaag cgatatccgt cgtcggtacg atcacggcca ataatgacgt
     2941 ggccatcgta tcggaaacgc aaggtcgtgt ggtgagcgtc aatgccaaag tcggcgatca
     3001 attaggcgcc ggatcggtca tcgcacaggt ggatgacgaa ctgaaaaaag ccaattatct
     3061 gcttgccgaa gcaaactatc aaaaaacaaa aaaagacctc gagcgcaacg aagaactttt
     3121 taaaaacaac gcgatctcga ccagccagat cgaatcttcg cgcctggctt tccaatcggc
     3181 cgaagcgcaa tacatcgtgg cacgccgtca gtacaatgat acgcgtatca cgactccgat
     3241 cggcggcatc gtttcgtccc gttcggttga tttcggaacg atggtcgttc ccggcaccgt
     3301 gattgccaat gtcgtggatg tttcgaaact gaaagtaaaa atccaattgg ccgagcacga
     3361 cgtatttaaa atcaaaaacg gcgataaagc tgtggtctcc accgatgttt atcccgggat
     3421 ggaatttgaa gcccgtgtag aaaacatcag cgccaaagcg gatgaagcgc acacctatcc
     3481 ggtcgaactg agcctcaata ataattccaa aaatccgctc aaagccggaa tgtttgcccg
     3541 tgttgacttt aaaacatttg tagaacgtac atccgtagtc atcgcacgcg aagccttagt
     3601 cggcagcttg aaaaaaccgc aagtattcgt agtcgaaggg gataaagccg tgctgcgcaa
     3661 tattgtggta ggcgttacat ccagtcacaa aatcgaagta ttgagcggtc tcaaagaagg
     3721 cgaaaacgtg gtgatcaaag gacaaaacaa tctccaggat caatccacca tccgtgtcgc
     3781 ccagtagtat tatggtattg ttttaatatg taacgctatc caacgaagca ggaaaaataa
     3841 aaaatgagta taacagaatt agccataaag cgtccgtccc tgatcgtcgt gatcttcggt
     3901 gcgcttacgg tgcttggtat tttcggatac aatcagctca gttatgaact gttgccaaaa
     3961 tttgcaccac cggtagtcac catcagtacg atctaccccg gcgcttctcc atccgaagtg
     4021 gaaaactccg tcagtaaacc gatcgaagaa gccgtctccg gtctggataa tgtctcccgt
     4081 atcaattcga cttcccagga aggtttttcg ctggtatttg ttcgtttgac ccagtctgcc
     4141 aatattgatc ttgcattgca ggaagcgcag cgcaaagtca acggtatcct tgcgaccatg
     4201 ccccgcgacg tcaaaacacc ggtcattacc aaatttgcat tggacgaatt cccggtattg
     4261 cgtatgggtg cgaccagttc gatgggtccg cgtgaatttt atcaatttct caaagaccgc
     4321 gtcgtaccgc gcatcgccaa gcagtccggt gtcgcacagg tcactttggt cggcggtaac
     4381 gagcgcgaaa tcaaaatcaa tctggatgcg caaaaaatca aatcttacgg cctatcactg
     4441 gcacgcgtag cccaaacgat caaagcctct aaccttgatt ttccgaccgg caatatcaaa
     4501 caagaaggca cccagtttgt cgttcgcgtt gcgggaaagt ttgaatccgt cgaagcgtta
     4561 agtttgcttg tcgtcggacg ctccaaacaa ggcggagata tcaagttatc cgatgtcgct
     4621 gaagtgcatg atggtcaaaa agacatgtca accttgagcc gaattaacgg aaacgaatcc
     4681 atcggtattt tggttcaaaa acaatccgat gccaatgccg tgcgcgtaag tgaacttgtt
     4741 cgtaaagagc tcgcgagcat ggaaaaggat tatgaaaacg taaaattaaa aattacgatc
     4801 gctcaagacg gttcccaatt taccgtggat gcggcaaatg cggtcaaatt tgatttgatg
     4861 ctggccatcg tactggttgc gttgaccatg tttttgtttt tgcacagcat tcgaaattca
     4921 atcatcgtga tgatcgcgat cccggcgtcg cttgtttcta ctttttttgc catgtacctg
     4981 ctgggattta ctctcaacct gatgaccttg ctcgcgttgt cgttagtcgt aggtattctc
     5041 gtggacgact cgatcgtcgt gcttgaaaat atttatcatt atttggaaaa aggcttgaaa
     5101 ccgcgcgatg cagcgatcac cggacgtaac gaaatcggtt tcgcggcgtt atccatcacg
     5161 ctcgtggatg tggtcgtatt tgtgccgctg gccatgacca caggccttat cggaaacatc
     5221 atgcgccagt tcgccatcgt agttacggtt tccacgatga tgagtttgtt tgtttcattt
     5281 acagtaactc cgcttctcgc ttcgcgtttt tccaaactgg aaagaatgac gaacggcact
     5341 tttttaggtc gatttgcatt atggtttgaa gccctctatg aagcatttaa aaatcattat
     5401 ttgcgtttgt tgcgttgggc tttatcccat cgcaaaaccg tagtcaccgc aacggtacta
     5461 ttgtttttct cgagctgtac gctggttccc tttggattta tcggtaatga attcgtcacc
     5521 cagggcgatc gcggtgaatt cgccgttttc ctcgaacttc cttcgggtgc gaccatcgag
     5581 tcaaccaatc atgcgacaca acaggccgaa aagatcatta ccgcgatgcc tgaagtgagt
     5641 aaagtattta ccaatgtcgg cgcatccagc gacgggtttc ttacgatgtc gtcgaataat
     5701 atttccgaat tgaatgttac gctggtggat aaatccgagc gcaccaaaac gacggatgaa
     5761 gtcggagcgt tgatcaaagc acgcatcgcc gaaattccgg gagttaaagt gcgcgtaaat
     5821 ccgatcggta tttttggtac ggccaatcaa accccgatac aaatcgtggt cagcgcaacc
     5881 aatgaagccg acatgaaaca cgccgcggac cgcgcgatgc aaattttggc cgcggtaccc
     5941 ggcgcaaccg acgtcaaact ttcagccgaa gacggcaaac ccgaaacgcg tatcgaagta
     6001 gatcgtcaaa aaatggcagc tttcggtctg accatcgccg atatcggaca aacgctgcgc
     6061 attgctttga cgggcgatga agattccaaa tttcgcgacg gcgccaacga atatacgata
     6121 cgcattcgcc tcgacgaatt tgatcgttct aatctcaacg acgtgcgcca tctgagtttt
     6181 atcaatagcc gtaacgaaca aatcgaactc caacaatttg catcgattta tcaaacggcc
     6241 ggcccttcca aattgcaacg tcaggatcgt ttcggttcta taaccgtgat gggacaggta
     6301 ctcggacgcc cgagcggtaa tgtggtgcag gatttccaaa aagccatcgc tttggaaaaa
     6361 gataaactcg gtaaggatgt tacattcact tatctcggcg atgaagaaaa ccgatcggaa
     6421 ggtttcggca gtatgggatt ggctttcctt gccggcgtac ttttcgttta tcttatcatg
     6481 gtggcgttgt acgattcata cctgtatccg tttgtggttt tattttcaat ccccgtggcc
     6541 ggcgtgggcg cgttgcttgc gcttgcctta gccatcaagt cgctctcgat cttttcatta
     6601 ctcggtatga tcatgatgat cggtctggtt gcgaaaaacg ccatcttgct tgttgatcgt
     6661 accaatgaca tgaaagcgca aggtatgcaa acctatgatg ctttgatgga agccggcagc
     6721 gcacgactcc gtccgattct gatgacgacg atggcgatgg tgatcggtat gttacctatt
     6781 gccttagcca gcggcgccgg tgcggaatgg aaaacaggtc tcgcctgggc tttgatcggc
     6841 ggtttgacga gttcgatgtt cctcacgttg atttttgtac cgatcgtgta tctgaagttt
     6901 gatgattgga aaaacagagt tcctcagttt ttcaaacgcc ttaccggtaa aaaaaatgaa
     6961 cccgtcatac tggctaccgg ctccaacggt cattatggac aagccgaaac ggtaaaatca
     7021 gaggcgtagg atcatgcggt agttttttga attacaaatt catatcgttt acaaacggtt
     7081 acacattcaa tcggtgtaac cgtttttatt ttactttttt atccttgcaa tatatccatc
     7141 gcccgtttaa tttacttcat acggtactcg ccatttcatt ttactatatc tatggtgtct
     7201 tcatcacagt gttttttctg cggcagtgcg gaaggttttg tgtttgtgca tggacattac
     7261 caatgccggc gctgtcacaa tgttcaaatc cgctgctgtg aaggcgagtc ctgtcagaca
     7321 acaaacaacg acacgaagga taacatcgtt ttttctaatt taaagcaggc cgtacatgga
     7381 taaatttatt cgccgtctca tcccgcaagc ggttgggata acatgcatgg tcatcggctg
     7441 gtggatcagt atcgttaatg tcggattgga taaatttgtt tccagtacga tcgtcaataa
     7501 atggacgatg atcggcttac tgctgatcat catcggcgcg tatatcccgc aaatctggat
     7561 ttttattttg aataaattaa ataaataacc gtctatgtgg aggtgtcgga ggaaacttcg
     7621 agcgttcaca gcaaaaaggg gccgtttcgc aaggaactac ccctttttcg tttttaaaac
     7681 attacaatta tgatcttcac aaatttcacg cgctcttgta ttctgcggct cgtgtgctgt
     7741 atatctatat cggctccgtt cttaaacgca caagaaaatg attcgttaca aacacgtatc
     7801 aaaaaaaatt ttctcgtcat ccccgctctg ttttacacac ctgaaacaaa gttaggctat
     7861 ggcgccattg cagctattta ttttcgtaaa ccgtttcctg aaaatgccaa acctacaacg
     7921 ctagtaccgt cggttatata
//
LOCUS       DAOQGF010000132         7921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4363_length_7921_cov_5.413171, whole genome shotgun sequence.
ACCESSION   DAOQGF010000132 DAOQGF010000000
VERSION     DAOQGF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_7921_cov_5.413171"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /gene="pnp"
                     /locus_tag="PK937_13730"
     CDS             <1..1147
                     /gene="pnp"
                     /locus_tag="PK937_13730"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNH33682.1"
                     /translation="ALVATTLGTKLDEQIIDGVDSVETYKRFMLHYNFPPFSVGEARG
                     SRGPGRREIGHGNLAERALKNMAPSDAEFPYTIRIVSDILESNGSSSMASVCGGSLSL
                     MDAGVPMKSPVAGIAMGLIKESEKYAILSDILGDEDHLGDMDFKVAGTTNGITACQMD
                     IKIRGISTEIMREALAQAKDGRMHILGIMNTALAQPRPDISRYAPKITTIKIKQDLIG
                     LVIGPGGKMIRHIIEASGATIDIEDDGTVKIGAVNSENAEIAINMIKALTEEPEIGKK
                     YSGPIKKITEFGAFVEILPGKEGLLHISEIDYKRVAKVEDYFKLGDVVEVLLQDMTQR
                     DGKTKYELSRKALMPKPEGHVEPEKDANRGQGHEHRRSSHHHKKDRH"
     gene            1183..2448
                     /locus_tag="PK937_13735"
     CDS             1183..2448
                     /locus_tag="PK937_13735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNH33683.1"
                     /translation="MKTLYDENNYTKVILDNGLTLVSQHMPHLRSVSIGVWITAGSRF
                     ESADTMGIFHFIEHSVFKGTKHRSALQIAKSLENVGGSINAFTSKEHTCFYANILSED
                     LTLAVDVLADLVIHARFDAKEINREKQVILEEIRDTEDTPEEFVQDYFYGQVYRNHPM
                     GYNILGTPQSVKVLDRKRIVDMYRKYYVPSNMVVSVAGNFDETTLQSVIAKKFVFPKN
                     LRNTSYWPAGFEKYTIRNTPSKTEVVEKKINQAHVCMGVTIHESYTDDHRFEYLVLNT
                     VLGGGMSSRLFQKIREKMGLAYSIYSFVDFLNDGGIFGVYWATDKNQAQKSVDTVKTE
                     LRKLADKPITVGELRMAKAQIKANLLFGLESTSTRMIRLAKNEIYLGKKINIGEITKL
                     IDRLTIRDIQKTGNTLAKLVDKMQISIVQ"
     gene            2477..3592
                     /gene="ald"
                     /locus_tag="PK937_13740"
     CDS             2477..3592
                     /gene="ald"
                     /locus_tag="PK937_13740"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNH33684.1"
                     /translation="MIIGVPKEIKTKENRVGLLPVGVAELKKHGHTVIVENNAGVGSG
                     FDNDAYVKAGAEIVPTPKDVYGRADMIVKVKEPIKPEYELIRENQVVFTYFHFAADRA
                     LTEAMIKSKCIAIAYETVQRSDRKLPLLDPMSEVAGRMAVHAGAKYLEKTFGGRGLLL
                     SGVPGTEPATVVVIGGGVVGTNAAKIAAGFGARVIMMDIDLYRLRYLDDIMPKNVHTM
                     MSNSYNIAEVIKHADLVIGAVLIPGAKAPHLITRDMLKTMKPGAVIVDVAVDQGGCVE
                     TTKPTTHEEPIYNIDGIVHYCVANMPGAVPFTSTVALTNATLPYAVQLANKGWEKAVK
                     ENHELLLGCNILNGKVVYKGVADAFDLPYVPVEQVVK"
     gene            3664..4014
                     /locus_tag="PK937_13745"
     CDS             3664..4014
                     /locus_tag="PK937_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNH33685.1"
                     /translation="MTEPIKKIYYTISEVCEKTDLKQYVLRYWETEFDALKPLKNRSG
                     NRVYTEDDIDNILKIKKLLYEDKFTIEGAKKRMLGDTDQLSVAQVPQNNNREKAHHLI
                     EDIRLDIETLLKQL"
     gene            4063..4794
                     /locus_tag="PK937_13750"
     CDS             4063..4794
                     /locus_tag="PK937_13750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH33686.1"
                     /translation="MSEIVKRHYDQLLADVYTWLYGGFDVQYTENLKFFKEQGIIPSG
                     KSIALDLGAGSGFQTLALNDLGFDVSSVDISDKLCSELGARAKGRKIEIYNSDILSFL
                     EVTTKEFEVIVCMGDTITHFDSKEDINKSIRLCSEKTVPGGSFILSFRDYSVELSGVS
                     RFIPVRSDAEKIFTCFVEYAPEKVYVHDILNTRISDKWEHKISVYPKLRLSVEWIKGV
                     LESNGLRITWSETSKGFVRLIARKG"
     gene            4889..7486
                     /gene="alaS"
                     /locus_tag="PK937_13755"
     CDS             4889..7486
                     /gene="alaS"
                     /locus_tag="PK937_13755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNH33687.1"
                     /translation="MAHVLTAKEIRSQFIRFFEERGHRFVPSSPVVPNDDPTLLFTNA
                     GMNQFKPIFLGTEKRDYTRAANSQKCIRVSGKHNDLEEVGHDTYHHTFFEMLGNWSFG
                     DYYKAEAIQWAWELFTKQWGLPKDRLYATVYKTDDEAVETWKKVTDINPDHILRFGEK
                     DNFWEMGDTGPCGPCSEIHIDLTPDGNGGKLVNAGSPEVIELWNLVFIQFNRNADRSL
                     TELPAKHVDTGAGFERVARVLQGKTSNYDIDLFTDIISGIEKVAGKNYAESAIQAPFR
                     VIADHIRMLTFSITDGAIPGNDGRGYVLRRILRRAALYGRKLELHEPFIYKLVTNVVG
                     AMGDAFPEIRARQEFVERVIKTEEENFNKTLDRGIEVFEDMACKLESKGIKQIPGADV
                     FKLYDTFGFPADMTRVMAAERGLSVDEDKFTEEMKAQKQRSREEGKKIFINKDIKWET
                     LGFEVKTKFIGYDVLESQAKPLKYYRDGKLIQMVFDQTPFYAESGGQVGDKGTVSIGN
                     HVFEIWDTQRVGDDVVHFADDFENFSLSSSASAQLSVSKDHREPTIKNHSATHLLHSA
                     LRSVLGEHVHQSGSVVEPYRLRFDFSHFEKITDDQLKKIETMVQERVFENIALQHHRN
                     IPIADAKKMGALSFFGDKYGEFVNVVQFGEYSKEFCGGTHVPSTGKIGFFKIVSESSI
                     ASGVRRIEAITGSVAEGLFRNQYALQESLRHTLNCKPEEISERIESLINDRKLLEKEL
                     SDLRLQSVTNQLDGLMASAVMVNGFKVITRKITLPSGTELRAVGDQLREKLASGVGVL
                     ATQDTILCVVSDDLIRDKKLHAGNLVKAIAQIAGGSGGGRPHSATAGIKDISKLDAAL
                     AKVSELVSM"
     gene            7505..>7921
                     /locus_tag="PK937_13760"
     CDS             7505..>7921
                     /locus_tag="PK937_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33688.1"
                     /translation="MKKNKTPKKTKPRPTKDLPPKKKHTGVPGGDGKTVQIQSKGRLT
                     KILSEKYRNGKKSLPIKPPSRTAPKETGTLMVSVSGIRGIIGESLTPEVITKYAAAFG
                     TWCNGGTVVVGRDSRVSGEMVKTLVTGTLLAAGCKIV"
BASE COUNT         2375 a         1571 c         1918 g         2057 t
ORIGIN      
        1 agcgcttgta gccaccacac ttggtaccaa attagatgaa caaattattg acggtgtgga
       61 ttcggtagaa acgtacaaac gattcatgct gcattacaat tttccgccgt tttcagtcgg
      121 cgaagcgcgc ggttcgcgcg gtcccggtcg tcgtgaaata ggacacggta acttagccga
      181 acgcgcactc aaaaatatgg cgccgtccga tgccgagttc ccctacacga ttcgtatcgt
      241 gagcgatatt ttagagtcca atggctcaag ctctatggct tcggtctgcg gcggttcgct
      301 ttcacttatg gatgcaggcg ttccgatgaa atcgcccgta gccggtatcg ctatgggact
      361 tatcaaagaa tcggaaaaat atgcgattct cagcgatatc cttggtgacg aagatcattt
      421 gggagacatg gattttaaag tagccggtac gaccaatggg atcacggcat gtcagatgga
      481 tatcaaaata cgtggtatct caacggaaat catgcgtgaa gctttggcac aggctaaaga
      541 tggacgtatg cacattctgg gtatcatgaa taccgcgctg gctcaacctc gcccggatat
      601 ttcacggtat gcgccgaaga tcacaaccat taagatcaaa caagatctta tcggtttggt
      661 gatcggaccc ggcggcaaaa tgatacgcca cattatagag gcatccggtg caacgataga
      721 tatcgaagat gacggtaccg tcaaaatcgg cgcagtcaat tccgaaaatg ctgaaattgc
      781 aattaacatg atcaaagcgc ttaccgaaga accggaaatc ggaaaaaaat atagcggccc
      841 gatcaagaag attacagaat tcggcgcgtt tgtcgaaatc ctgcccggaa aagaagggct
      901 tctgcatata tcggagattg attacaagcg cgttgctaaa gtagaagatt acttcaaact
      961 tggtgatgtg gtcgaggttc ttttgcaaga tatgacgcag cgggatggta aaaccaaata
     1021 tgaattgagc cgtaaagcgc ttatgcccaa acccgaaggt catgtggaac ctgaaaaaga
     1081 tgccaatcgc ggacaaggtc acgaacatcg ccgcagtagt catcatcaca aaaaggacag
     1141 acactaagcg tgccacgtaa tttcatagtc ccggaaacgg gaatgaaaac tttatacgac
     1201 gagaataatt ataccaaggt cattctggat aacggactga ccttggtttc tcagcatatg
     1261 ccgcatctgc gttcggtatc tatcggagtg tggattacag ccggaagccg gtttgaatca
     1321 gccgatacga tggggatatt tcactttatc gaacatagcg tattcaaagg cacgaaacac
     1381 cgtagcgcgt tgcaaatcgc caaaagttta gaaaatgtcg gcggtagcat caatgctttt
     1441 accagcaaag agcatacgtg tttttatgcg aatattctta gcgaagattt gaccttagcc
     1501 gtggatgtgc ttgccgactt agtgattcat gcacgatttg atgccaagga aataaatcgc
     1561 gaaaaacagg tgattctaga ggaaattcgt gatacggaag atacgcccga agagtttgta
     1621 caggactatt tttatggcca ggtgtatcga aatcatccga tgggttataa tatccttgga
     1681 acaccgcaaa gcgtaaaagt tttggatcgc aaacgcatag tggatatgta tcgtaaatat
     1741 tatgtaccat ccaatatggt ggtgtctgtt gcaggtaatt ttgatgagac cacgctgcaa
     1801 tcggtaattg ccaaaaaatt tgtatttcca aaaaatttgc gtaatacttc ctactggccg
     1861 gcgggtttcg aaaaatatac catacgaaat actccgtcaa aaactgaagt agtagaaaag
     1921 aagatcaatc aagcacatgt gtgtatggga gtgacgattc acgagtccta taccgatgac
     1981 catcgttttg aatatctagt tctcaatacc gtgcttggcg gcgggatgag ttcgcgttta
     2041 tttcaaaaaa ttcgtgaaaa gatgggtttg gcgtattcca tatacagttt cgttgatttt
     2101 ttaaatgacg ggggcatttt tggagtgtat tgggccaccg ataaaaatca ggcccaaaaa
     2161 agtgtggata cggtaaaaac cgaattacgt aagcttgcag ataaaccgat tacagtcgga
     2221 gaattgcgta tggccaaagc gcaaatcaaa gccaatcttc ttttcggtct tgaaagtacc
     2281 agtacgcgca tgatacgttt ggcgaaaaat gaaatttatc ttggcaagaa aataaatatc
     2341 ggtgaaatca ctaaactgat tgatcgactt acaatccgag acatccaaaa aacagggaat
     2401 acgcttgcaa aacttgtaga taaaatgcaa atttcaattg ttcaataatc tggaccaaat
     2461 tctaggagac gaagtcatga ttataggtgt accgaaagaa atcaaaacca aagaaaaccg
     2521 tgtcggtctt ttgccggtag gcgtagccga gctcaaaaaa catggtcata ctgtgatagt
     2581 cgaaaataat gccggtgtag gcagcggttt cgacaatgat gcatatgtca aagccggggc
     2641 tgaaatcgta ccgaccccta aagatgttta cggtcgtgct gatatgatcg tcaaggtcaa
     2701 agaaccgatc aaaccggaat acgaattgat tcgcgagaat caggttgtat ttacgtattt
     2761 tcactttgcg gcagatcgcg ctttgaccga agcgatgatc aaatccaagt gtattgcgat
     2821 cgcttatgaa acggttcaac gttcggatcg taagttgcct ttgcttgatc cgatgtcaga
     2881 agtagcagga cgtatggcgg ttcatgccgg cgctaagtat cttgaaaaaa cgtttggagg
     2941 ccgcggatta cttctgagcg gggttccggg cactgaaccc gcaaccgtcg ttgtgatcgg
     3001 tgggggtgtt gtaggtacca atgctgcaaa aatagccgcc ggtttcggtg cgcgtgtcat
     3061 tatgatggat attgatttgt atcgtttgcg ctatttggat gatatcatgc ccaaaaatgt
     3121 acataccatg atgtcgaata gttataatat cgccgaagtg atcaaacatg ccgatctcgt
     3181 gatcggagct gttttgatac ccggagccaa agccccgcac cttatcacac gcgatatgct
     3241 aaaaaccatg aaacccggtg ccgtgatcgt ggatgtggct gtggatcagg gcggttgcgt
     3301 tgaaacaact aaacctacga cacacgaaga accgatctac aacatagacg gtatcgtaca
     3361 ctattgtgtt gccaatatgc ccggtgcggt tccgtttacg tcgacggtcg ctttgaccaa
     3421 cgcaacactg ccgtatgccg tacaattggc caataaaggc tgggagaaag ccgtcaaaga
     3481 aaatcacgaa cttttactcg gatgcaatat cctcaatgga aaagtcgttt acaaaggtgt
     3541 agctgatgct tttgaccttc cttatgttcc ggtagaacaa gtcgtcaaat aatcgtttat
     3601 acaaagagcg gtaatctgaa aaggttaccg ctcttgtttt taagcctagt ccggagtgag
     3661 tctatgaccg aaccgatcaa aaaaatatac tatacgatca gtgaagtttg tgaaaaaacc
     3721 gatctgaagc agtatgtact tcggtattgg gaaacggaat ttgatgcgct gaagccgctt
     3781 aaaaaccgtt ccggtaacag agtttatacg gaagacgata tagataatat cctcaaaata
     3841 aagaaactac tgtatgagga taagtttacg atcgaaggcg ccaaaaaaag aatgttgggg
     3901 gatacagacc aactctcggt tgcacaggtt cctcaaaaca ataataggga aaaagcgcat
     3961 catctgatcg aagatatccg actggatata gagacgctct taaagcaatt gtaatccgta
     4021 aaacactcct taagccttac ttcgcacagt tttaaaaaag atatgtcaga aattgtcaaa
     4081 agacattacg accagctatt ggcagatgtc tatacttggt tgtatggcgg gtttgatgtc
     4141 caatacactg aaaatcttaa attttttaag gaacaaggta ttatcccatc cggcaaaagt
     4201 atagctttgg acttgggagc aggtagcggg tttcaaacat tagctctaaa tgatttaggt
     4261 tttgatgttt cgagtgttga tataagtgac aaactttgct ccgaattggg agcacgtgca
     4321 aaaggccgaa aaattgaaat ttataattcg gatattctaa gctttttgga agtcacaacg
     4381 aaagaatttg aagtaattgt ttgtatggga gatacgatta ctcactttga ttcaaaagaa
     4441 gatattaata aatctatcag actatgctcc gaaaaaacag ttcccggagg ttcctttatt
     4501 ttatcttttc gcgattacag cgtggaactt tcaggtgttt cacggtttat tccggttcga
     4561 tcagatgcag aaaagatatt tacgtgtttt gttgagtatg ctcccgaaaa agtttatgtg
     4621 cacgatattt taaataccag gattagcgat aaatgggagc ataaaatcag tgtttatcct
     4681 aaattgaggc tctcggtgga atggataaaa ggggttttgg agtccaacgg tctgcgaata
     4741 acatggtccg aaacgtctaa aggttttgtg cgactaatcg cacgcaaggg ctaaattgcc
     4801 gattttattt gaaattgttt tgataatccg cacagcgttt atatatttat atctgtaaaa
     4861 tcaattattt ggagttataa tagatttcat ggcacatgtt cttacagcga aagaaattcg
     4921 tagtcaattt atccgttttt ttgaagagcg cgggcatcgt tttgtaccat ccagtccggt
     4981 tgttcctaat gacgatccga cgttactttt tactaatgca gggatgaacc agtttaagcc
     5041 catttttttg ggtaccgaaa aacgagacta tacacgagcg gccaattcgc aaaaatgtat
     5101 ccgcgttagc ggcaaacaca acgatctcga agaagtaggg cacgatacat accaccatac
     5161 attttttgaa atgttgggta actggtcatt tggggattat tataaagccg aagcgataca
     5221 gtgggcatgg gaacttttta cgaagcagtg gggattgcct aaagatcgcc tttatgcaac
     5281 cgtgtacaag acggacgatg aagcggtcga aacctggaaa aaagttacgg atatcaatcc
     5341 ggatcatatt ttgcgatttg gagaaaaaga taatttttgg gaaatgggtg atacggggcc
     5401 gtgcggaccg tgttcagaaa tccatattga tcttacaccg gatgggaatg gcggcaaatt
     5461 ggtcaacgcc gggtcacccg aagtgatcga actttggaat ctggtgttca tacagtttaa
     5521 tcgaaatgcc gatcgcagcc tgactgaact gccggctaaa cacgttgata ccggtgccgg
     5581 ttttgaacgc gttgcacgag ttttacaggg caaaacttcg aactacgata ttgatttgtt
     5641 tactgatatt atttcaggaa ttgaaaaagt agcgggaaaa aattacgccg aatccgctat
     5701 tcaggcgccg ttccgggtca ttgccgacca catccggatg ttgacatttt ctataacgga
     5761 cggcgctata ccgggcaacg atggccgagg ttatgttttg cgacgcattc tgcggagggc
     5821 tgcactatat ggtcgtaaac tcgaattgca tgaaccgttt atttacaagc ttgttaccaa
     5881 tgtcgtcggc gcgatgggtg atgcttttcc tgaaatccgt gcacggcagg agtttgtcga
     5941 gcgcgtaatc aaaaccgaag aagaaaattt taataaaaca cttgatcgcg gtatagaagt
     6001 tttcgaagac atggcgtgca aattggaatc taaaggcata aaacaaatac cgggtgcgga
     6061 tgtattcaaa ctttatgata cgttcggatt tccggcggat atgacgcgtg tgatggcggc
     6121 cgagcgcggt ctttctgtag atgaagataa gtttacggag gaaatgaagg cgcaaaaaca
     6181 acgttcgcgt gaagaaggaa aaaaaatatt tatcaacaaa gacataaaat gggaaacgct
     6241 cggattcgag gttaaaacca aattcatcgg gtatgatgtt ttggaaagtc aggccaaacc
     6301 gttgaagtat taccgtgatg gtaaattgat acaaatggta tttgatcaaa cgcctttcta
     6361 cgcggagtcc ggtggtcaag tcggcgacaa aggcacggtg tcgatcggaa accatgtatt
     6421 cgagatttgg gatacgcaac gtgtgggtga tgatgtcgta cattttgcgg atgattttga
     6481 gaatttttca ttatcgtcat ccgcatccgc acagctttcc gtttccaaag atcatcgcga
     6541 gccgaccatc aaaaatcatt cggccacgca tttattgcat tcggcgctgc gttcagttct
     6601 cggtgaacac gttcatcagt ccggttccgt agttgagcct tatcgtttac gatttgattt
     6661 ttcgcatttt gaaaaaatca cggatgacca gttgaagaaa atcgaaacca tggtgcagga
     6721 aagagtattt gaaaacattg ctttgcagca ccatcgcaat ataccgatcg ccgatgcgaa
     6781 aaaaatgggt gcgttatcgt ttttcggaga taagtacggt gaatttgtca acgtggtgca
     6841 gtttggcgaa tacagcaaag aattttgcgg cggcacacac gtgccttcga cggggaaaat
     6901 cggttttttc aaaatcgtgt ccgaatccag tatcgcctcc ggtgtgcgcc ggattgaagc
     6961 cattaccgga tccgtagcgg aggggttatt ccgaaatcaa tatgcattgc aagaatcatt
     7021 acggcacacg ctcaattgta aaccggaaga aatctcggaa cgcatcgagt ctttgatcaa
     7081 tgatcgtaaa cttcttgaaa aagaactgtc ggatcttcgt ctgcaaagtg tgacaaacca
     7141 attagacggt ttgatggcgt ccgccgttat ggttaacgga tttaaagtaa ttactcgtaa
     7201 aataacattg ccttcaggaa cggaattgcg cgcggtgggt gatcagctgc gtgagaaact
     7261 ggcttccgga gtaggcgtcc tcgccaccca agatacgatc ctttgcgtgg tcagtgatga
     7321 tctgatccga gataaaaaac tgcacgccgg aaatttagtt aaagctattg cacaaatagc
     7381 cggtggaagc ggcgggggga gaccgcattc ggcaacggcc ggcattaagg atatttccaa
     7441 gctggatgcg gctttagcaa aagtaagcga actcgtgtcg atgtaatttt ttagaaattc
     7501 aacgatgaaa aaaaataaaa cacccaaaaa aacaaaacca agaccgacca aagaccttcc
     7561 tccaaagaag aaacataccg gtgtccccgg tggagatgga aaaactgttc aaattcaatc
     7621 taaaggccga ctaacaaaga ttctgtccga aaaatacaga aacggaaaaa agtcactacc
     7681 gatcaaaccg ccttcacgca ccgcaccaaa agaaaccgga acgttgatgg ttagtgtttc
     7741 cggcatacgt ggtatcatcg gtgaaagtct tacgccggaa gtgatcacaa aatacgcggc
     7801 cgcttttggc acgtggtgta atggcggtac ggttgttgtc ggtcgggatt cgcgggtgtc
     7861 cggcgagatg gtcaaaacac tcgtgaccgg tacattgctt gccgcgggat gtaaaatcgt
     7921 a
//
LOCUS       DAOQGF010000133         7917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4368_length_7917_cov_6.926736, whole genome shotgun sequence.
ACCESSION   DAOQGF010000133 DAOQGF010000000
VERSION     DAOQGF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4368_length_7917_cov_6.926736"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PK937_13765"
     CDS             <1..404
                     /locus_tag="PK937_13765"
                     /inference="COORDINATES: protein motif:HMM:NF026866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 23 domain-containing
                     protein"
                     /protein_id="HNH33689.1"
                     /translation="NWSNDLPFSDKGRTASAEVGFGQNLIGFNVFTSEHGDVDKAFES
                     AQNGSAYVSEVHGVNEAGSYNDRGTPYFSAFYFARRSGRNVFRTGVNAPWVQNVFQNG
                     AHQLIGVPYFIPGNYPARPYGYFGRYNPFIY"
     gene            453..1043
                     /locus_tag="PK937_13770"
     CDS             453..1043
                     /locus_tag="PK937_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33690.1"
                     /translation="MKFYIIILSLFFASCVMYSVHDELWSEKLNTTKSKIIHSKTNGY
                     YKLSNTSQKDIRMPSDFILYQKGYFAGHGYYWAKRKSPDKFLGKRYGNWGKYNISQSD
                     SIFIQYYTVVDALGSLIGALFHLSVSVATGTVKNDTTILIHSLTYHDIREKNDLQMFK
                     VENYSPPLQFSFVPLDSVPSADGNWLEEWLKKKKEY"
     gene            1284..2417
                     /locus_tag="PK937_13775"
     CDS             1284..2417
                     /locus_tag="PK937_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH33691.1"
                     /translation="MYDGLNSIAVKRKDGGTSEYVYGPTGLIAANINGTWYFVLKDHL
                     GSARTIVDQSGSSKAAIDYAPYGAIIRTSGSPNIAYQFTGQEYDSTSGLWNYKARMYD
                     AELGVFYAVDPAGQFGSAYIYNNDEYAQNNVTSQKDSVEINEMASPFLYTGNNPVRYV
                     DRNGELFIIDDYAIGFVKGLAKGKNPFKTGWKHAQNSALIWGGLFQGSPTQILSRFTW
                     ELPQTVVGFTGAHGTNMFGNVKSVKYYDGATVIRTGSKGYWGITLGSYIIGSREMKVG
                     IGTSLGHQLFMHEYGHYLQSQRNGPLYLTKYGIPSLANAAFGSNHNKYWTETDANRRA
                     ERYFRVQHGFTQWNDSYYPRASQLSNGSWWEYPLWPISFFWNF"
     gene            2433..2843
                     /locus_tag="PK937_13780"
     CDS             2433..2843
                     /locus_tag="PK937_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33692.1"
                     /translation="MEIKTNFFIVILLSYNSLSCDGCSDALGFDSRLEVINNSTKKIH
                     FDGGFTYPDTSIGEILSGEDIKPNSQRVFGIQGRWEERFEDLPAETLMIFIFDADTLD
                     QVPWEKVRSEYKILRRYDLSLQDLKDRDWKVVYP"
     gene            2890..3468
                     /locus_tag="PK937_13785"
     CDS             2890..3468
                     /locus_tag="PK937_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33693.1"
                     /translation="MKFITWLFLTISGSIAWAGTLDTISIGFSIGRNSYKKDQKEFFI
                     KTEIRLLNHSFFSKLGVQSRQYDLSFDGVRNLKAKSLGLFAEYIRYLWDDNLFAGMRW
                     ELINFNRLTTASRDRVKREHSYDMTSFYTGTCIFLQIGFDLYVTKNLAITIYPQYGFQ
                     QFMISNAESKPVKEDHYKAIYDVNIGIVWGSK"
     gene            complement(3591..4091)
                     /locus_tag="PK937_13790"
     CDS             complement(3591..4091)
                     /locus_tag="PK937_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNH33694.1"
                     /translation="MKYLPRRKFLSISAAGIPLFLTSKTALLSQTKKEKGPALDPALV
                     KDFVAIAHKDTAKTIAMLDTNPDLLNAVHNLGGWDWEDALGAAGHVGNPELATALIDR
                     GARMTICVAAMLGKIQIVRPMIEAFPAMKKAVGPHNISIFRHAKAGGDNAREVLDYLV
                     EIGVKE"
     gene            4304..6700
                     /locus_tag="PK937_13795"
     CDS             4304..6700
                     /locus_tag="PK937_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33695.1"
                     /translation="MFRNFYVIALRNLMKNKLFSFINVFGFSLSLSVCLLIISITIDQ
                     YSYDQFHPLGEDTFRINTIAQRKNGDHESYASSPAPLASLIRSENPFVRHTVSLNRSL
                     NGSMTSDDIKLMINGYFTESSFFEVFGFTLLKGNADALNDPRSLMISEETGQRFFGTE
                     NPIGKTVTIGSYGEFVVRGVIKKMDNKSHLTFDALGSLEFLKTWNGGSILAHEEWKNY
                     YSTYTYVVVTPGTDAAMLQAGLDRIPEKVYRGITLETRDAGYAFKAQSLQAITPGPML
                     SNSTSGGMPQAVLIFLMVLAGIGMFAALFNYANLTLARSLTRAREVGIRKVSGATRAQ
                     LIFQFMGESVIITLVSFLVAETLLRVFLVPAFQSLSFTSELDIRFDPSAGTYLLFAGF
                     ALCIGLLAGIIPAMVLSSFNPAVVIKDISKIRMFSGLTLRKTILISEFVVTIILLIVL
                     TTVYRQSEFASKMEYYGFDWRNTINVQLSGSQAPVIADEMARFPGVVSYSFASHAMGT
                     WADHTVDVQTDPTKEASVVRDYSVDEHFIETMKITLLTGENLHRSESTGDKVLVNEQF
                     VKSLQLSNNSDAVGKVIYLDKSRPVTIAGVIKDFVFKPVTYAMEPVILTYEPAQWSLV
                     HFKITNENYKTVISFFKSVWKKFNPSIEFPYQVYSDELISVYSIFTDLGRIIGLLSLL
                     ILTIAMLGLLGVASYSVETRTREIGIRKVLGASAMQLINLLSKQYLKLIGIAMLIGLP
                     VSLFLSNMILESFAYRTEFGIGIILPAVILLGFLVGLTILSQAWRGAHHNPVTALRQE
                     "
     gene            6981..>7917
                     /locus_tag="PK937_13800"
     CDS             6981..>7917
                     /locus_tag="PK937_13800"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNH33696.1"
                     /translation="MHRIVTPAEMQTIDRETIERHSIPGRELMARAGEVIFLTLTKRI
                     PDLASKRVLILCGKGNNGGDGYVLARCMISGGMTPKLIIAAEKKHIGGDALWHLEKLE
                     RTGVVVDASDAFEAPTEEYDVIVDALLGTGGRGHLSEPIKSWIEWINKQRFQKNAFIV
                     SVDIPSGLDGETGLFSDTAVTAHLTVTIGLPKRGLLFNDGKKYTGHLVIKDIGFPEVL
                     LSGGLWQFINQSDVAFHLPIRRHNSNKYDFGKLLIIAGSRGMSGAALMTARAAMRSGA
                     GVVRVAIPKSIAHVIEQGLPEAMTVHLPETEDGVIS"
BASE COUNT         2313 a         1449 c         1772 g         2383 t
ORIGIN      
        1 tcaactggtc gaacgatctg cctttttccg ataaaggaag aactgcatca gcagaagttg
       61 gatttggaca aaacttaata ggatttaatg tgtttacctc ggagcatggt gatgtagaca
      121 aagcttttga aagcgcccaa aatgggtctg cttacgtttc tgaagtccac ggagttaatg
      181 aagctgggtc ttataatgac agaggcactc cttattttag tgcattttat tttgcaagga
      241 gatcaggtag aaatgttttc aggacaggag taaacgcacc atgggttcaa aatgtatttc
      301 agaatggcgc tcatcagcta ataggtgttc catattttat tccaggtaac tatccagcta
      361 gaccttatgg ttattttggg agatacaatc catttattta ttgaataatc aaatattttc
      421 tacgttgact ctataaataa aaaaggcgcg ttatgaagtt ctatataata atcttaagcc
      481 tgtttttcgc ttcatgtgta atgtattctg ttcacgatga attatggtcg gaaaaattga
      541 atacaacgaa aagtaaaatc attcattcaa aaacgaatgg atactataag ttatcaaaca
      601 cttctcaaaa agatattcgt atgccttctg attttattct gtatcaaaaa ggttattttg
      661 caggacatgg ttactattgg gcaaaacgta aatcgcccga taagtttctt ggtaaacggt
      721 atggaaattg gggtaagtat aatataagtc aaagtgatag tatctttatt caatactata
      781 ctgttgttga tgctttgggt agtctaatag gcgctttgtt tcatttgtca gtaagtgttg
      841 ctactggcac ggtaaaaaat gacacgacaa ttttaattca tagtctgact tatcatgata
      901 ttcgtgagaa aaatgattta cagatgttta aagttgaaaa ttattctcct ccactgcaat
      961 ttagttttgt tcctcttgat tcagttccat cagcggatgg aaattggctt gaggaatggt
     1021 taaaaaagaa aaaggaatat tgatagaata atggtttgtt gttatttaca attgccgatg
     1081 aaatcataac tcgatatttc tttacatcaa ttgatacgcc aattttttat atttcattgt
     1141 acatattctg gagcaagttc cgtggaagag agttcgttct gaatacaaaa cacttaagcg
     1201 ttatgaccta agtttgcagg atttgaaaga ccgagattgg aaagttgttt atccttaaga
     1261 tttgaagtcg ggtcttcata tatttgtacg atggccttaa tagcatcgcc gtaaaacgca
     1321 aagacggcgg tacgagcgaa tatgtctatg gtccaacggg actgatcgcg gcgaatatca
     1381 atgggacgtg gtattttgta ctcaaagatc atttgggttc ggcgcgtacg atcgtggatc
     1441 agtcaggaag tagtaaagcc gcgatagact atgcgcctta tggagcgatc attcgcacat
     1501 cgggctcgcc caatatcgcg tatcagttta ccggacaaga atatgacagc acgtccgggt
     1561 tgtggaatta taaggcacgg atgtatgacg cggagctggg tgtgttttat gccgtggatc
     1621 cggcggggca gtttggtagc gcttacattt ataacaatga tgaatacgct caaaacaatg
     1681 taacgtcaca aaaggattct gtagaaatca atgaaatggc atcgccgttc ctttataccg
     1741 ggaataatcc agtccggtat gtagatcgta atggcgagtt attcatcatt gatgattatg
     1801 ccattggttt tgtgaaagga ttagctaaag gaaagaaccc ttttaaaact ggatggaaac
     1861 atgcgcaaaa ttcggcattg atatggggcg gtttatttca aggtagccct acgcaaatat
     1921 taagccgatt tacttgggaa ctgccgcaga cggttgttgg ctttaccggt gcacacggaa
     1981 caaacatgtt tgggaatgtg aaaagtgtaa aatactatga tggagctact gtaatacgga
     2041 caggtagcaa aggctattgg ggaattacgc ttgggagcta tattattgga agtagagaaa
     2101 tgaaagttgg tataggaact tcacttggac atcagttatt catgcatgaa tacggccatt
     2161 acttacaaag ccagagaaac ggtccattgt atctgacaaa atatggtatt ccaagtttgg
     2221 ccaacgctgc atttggtagt aaccacaaca agtattggac cgaaaccgat gccaatagaa
     2281 gagctgaaag atatttcaga gtgcagcacg ggttcaccca gtggaatgat agctattacc
     2341 ctcgcgcatc acaattgagt aatggatcat ggtgggaata tccactgtgg cctatatcat
     2401 ttttttggaa tttttaaaaa aggagaactt gtatggaaat taaaacgaat ttttttattg
     2461 taatactttt aagttacaac tcacttagct gcgatgggtg tagtgacgct ttaggatttg
     2521 attcacgctt agaagtaata aataattcta caaaaaagat acacttcgat gggggattta
     2581 catatcctga tacttccatt ggagaaattc taagcggcga ggatatcaaa ccaaattctc
     2641 aaagggtatt tggtatccaa ggaagatggg aggaacgttt tgaagactta ccagcagaaa
     2701 cgctcatgat tttcatattt gacgcagata cattagatca agtaccgtgg gaaaaagttc
     2761 gttcagaata caagatactg aggcgttatg acctgagctt gcaagattta aaagatcggg
     2821 attggaaggt cgtttaccct taaattcaag gggcttattg agttaataat caaaagggaa
     2881 aaagaacaca tgaaatttat cacatggctg tttctcacta tatcgggaag tattgcatgg
     2941 gccggaactt tggatacgat ttctatagga ttttcaatcg gtcgtaacag ttataaaaag
     3001 gatcaaaagg agtttttcat aaaaacggaa attcggcttt tgaatcactc ttttttcagc
     3061 aaattgggag tccaaagccg gcaatatgat ttatcatttg atggagttcg gaatctcaaa
     3121 gccaaatcgc tcggtttatt tgcggaatat attcgttatc tttgggatga taatttattt
     3181 gccggaatgc gttgggagtt aatcaatttt aaccgactta ctacggcctc acgcgatcgt
     3241 gtgaaaaggg aacactccta cgatatgaca tcattttaca ccggaacgtg tatattttta
     3301 cagattggtt tcgatctgta tgtgactaaa aatctggcga tcacgattta cccgcagtac
     3361 gggtttcagc agtttatgat atctaatgcg gaatccaaac cggtcaaaga agatcattat
     3421 aaagcaatct acgatgttaa tataggcata gtatggggca gcaaataaca taaatcttct
     3481 ctgaatagac gtactcgcta ttttgatgcg aatcaaaaaa aagatataaa agtgtgctgc
     3541 gtgaaataac gaaaggaaaa ttctatctat aaaattacca tagttggtct ttactctttt
     3601 actccgattt caaccaagta gtctagaact tcccgtgcat tgtcaccgcc ggcttttgca
     3661 tgcctgaaaa tggatatgtt atgcggccct acggcttttt tcatcgcggg aaaagcttcg
     3721 atcatagggc gtacgatttg aattttcccc aacatcgctg caacacatat agtcatcctc
     3781 gcgccccgat ctatcaatgc cgtagccaat tccggattac cgacatgtcc cgcagcgccc
     3841 agtgcatctt cccaatccca tccgccgaga ttatgtacgg cattcaaaag atcaggatta
     3901 gtgtccagca tagctatggt tttggctgta tctttgtggg ctatagctac aaaatccttt
     3961 accagcgccg ggtccaaggc aggccctttt tcctttttag tctgtgacaa aagagcggtc
     4021 ttgctcgtca aaaacaaggg tattcccgct gctgaaatgg acagaaattt tctgcgaggt
     4081 aagtatttca taattatcct ttcgagaata tatgaagaca atactgttcg gagaggtaaa
     4141 ataaaaacga agacaggtaa taactctgaa cgtaaaagac tttaaattgt ttcacaaatt
     4201 atatggtgat gttcaataca tttgaattat tgcaactttt tggctttaaa cccgtcatat
     4261 ttatattacc ggtagtattt ccaaatttcg tgaaagggta gatatgttcc gaaattttta
     4321 tgtaattgct ttgcgcaatc tgatgaaaaa caaattgttt tcgtttataa atgtttttgg
     4381 tttttcattg agcctgtcgg tgtgtttact gattatttct attactatag atcagtattc
     4441 atatgatcaa tttcatcctt taggtgaaga tacgtttcgc attaatacaa ttgcccaacg
     4501 taagaatggg gatcacgaat cgtatgcttc atccccggcg ccgcttgctt ctctgattcg
     4561 ttctgaaaat ccgtttgtca ggcataccgt aagcctcaat cgcagcctaa acggttctat
     4621 gacgagcgat gatatcaaac ttatgattaa cgggtatttt acggaatctt cattttttga
     4681 agttttcgga tttacacttt tgaaaggaaa tgccgatgca ttaaacgatc cccgaagttt
     4741 gatgatttca gaagaaacag ggcagcgctt ttttggaaca gaaaacccga ttgggaaaac
     4801 tgttacgata gggtcgtatg gtgagtttgt agtcaggggt gttataaaaa aaatggacaa
     4861 taaatcccat cttacgttcg acgcattggg gtctttggaa tttctgaaaa catggaacgg
     4921 cggcagtatc cttgcgcacg aagaatggaa aaattactac agtacttata catacgtggt
     4981 ggtaacaccg ggaacggatg cggcaatgct tcaggcggga cttgatagga ttcccgaaaa
     5041 agtgtatcgc ggaataacat tggaaacccg ggatgcgggt tatgcgttta aagcccaatc
     5101 gcttcaagcg atcacgcccg gtccgatgtt atccaatagt acttcaggcg gaatgccgca
     5161 agcggttctt atttttttga tggttttggc cggaatagga atgtttgccg cgttattcaa
     5221 ttatgccaat cttacactgg cgcgctcatt gacgcgtgcg cgggaagtcg gtatccgcaa
     5281 agtttcaggg gcaacgcgtg cgcagttgat tttccagttc atgggcgaat cggttatcat
     5341 tacattagtc tcgtttttgg tagctgaaac actgctgcgg gtatttcttg tgccggcttt
     5401 ccaaagttta agttttactt cggaactgga tattcgattt gatccctcgg caggaaccta
     5461 cttattgttc gcgggatttg cgttgtgcat cggattactg gcaggaatta ttccggccat
     5521 ggttttatcc tcattcaatc cggcggtagt cataaaagat atttcaaaaa ttcgaatgtt
     5581 ttcaggtttg acgcttcgta aaacaattct tatttcagaa tttgtcgtaa ccattattct
     5641 tcttatcgtc ctcactacag tatatcgtca gtcggagttt gcttcaaaaa tggagtatta
     5701 cggattcgat tggcgcaata cgatcaacgt gcaactttcg ggttcccagg cacccgtgat
     5761 tgccgatgaa atggcgcgtt ttccaggcgt agtttcttat agttttgcgt cccatgcgat
     5821 gggtacatgg gccgatcaca cggtagacgt tcagactgat ccaaccaaag aggcttctgt
     5881 cgttcgagat tatagcgtcg atgaacattt cattgaaacc atgaaaatta ctttattaac
     5941 aggcgagaat ctgcatcgga gtgaaagcac aggggataaa gttctcgtca atgaacaatt
     6001 tgtcaaatca ctgcagcttt ctaataatag cgatgccgtc ggaaaggtta tttatctgga
     6061 caaatcaaga cctgtaacta ttgcaggtgt gatcaaagat tttgttttca aaccggtaac
     6121 gtacgcaatg gagcccgtga tactaacgta tgaacccgcg caatggtcat tggttcattt
     6181 taaaataacg aacgaaaatt acaaaaccgt gatttctttt tttaagtcag tatggaaaaa
     6241 atttaacccg tccatcgaat ttccatatca agtttattcg gatgaactga taagcgttta
     6301 tagcatcttt acagatttgg ggcgtatcat cggtttatta tctttgctga tattgactat
     6361 cgcgatgctg ggattactcg gagtggcttc ctacagtgtc gagacacgca cccgtgaaat
     6421 cggtattcgc aaagtactcg gcgcttcggc tatgcagcta atcaatcttt tatctaagca
     6481 gtatcttaaa ctgatcggaa tagccatgct gatcggattg cctgtgagtt tatttttatc
     6541 gaacatgata ctggagtctt ttgcgtatcg tactgaattt gggataggta ttatattacc
     6601 ggctgtgata ttattaggat ttttagtcgg cttgacgata ctgtcgcaag catggcgagg
     6661 tgcacatcat aatcccgtga cagcgctacg tcaggagtaa atcgagaaac tattctgttt
     6721 aatttaaaag ataataaaat aggcgaagtg tattttgtgc taatgtgctt ttcatggttg
     6781 acaaaaaaaa gaaattaaaa tcagaacggt atttgatcat attcaaatta tttatacgaa
     6841 actaagtaac gcgttaaggg acattctgtt ttggaatgat tttaaaatac aaaacccgca
     6901 tttaagtcta tcaatctggc attttgttct agcgaatcgt attttccgtt aatacttaat
     6961 tccagcgtaa tttctgatat atgcatcgta tcgtcacgcc cgcagaaatg cagaccatag
     7021 atcgcgaaac tatcgaacgt catagtatcc cggggcgcga actgatggcg cgtgcagggg
     7081 aagttatttt tttgacattg acgaaacgta tacccgattt agcatcgaaa cgagttctca
     7141 ttctttgcgg aaaaggcaat aatggcggag acggttatgt gttagcccgg tgcatgattt
     7201 ccggcggtat gacgccaaaa cttatcatcg ctgcggagaa aaaacatatc ggtggtgatg
     7261 cactgtggca tctcgaaaaa ttggaacgaa caggcgtcgt agtcgatgct tccgatgcgt
     7321 ttgaagcacc aacggaggaa tatgacgtca tcgtagatgc attacttggc accggtggcc
     7381 gcgggcattt gagcgaaccc ataaagtctt ggatcgaatg gatcaataaa caacgttttc
     7441 aaaaaaacgc atttattgtg tccgtagata taccttcggg acttgatggt gaaaccggat
     7501 tgttttcaga tacggcggtt accgctcatc tcaccgtgac gattggatta cctaagcgag
     7561 ggttgctttt taatgacgga aaaaaataca ccgggcatct tgtaataaaa gatattggtt
     7621 tcccggaggt attgctctcg ggcggtttat ggcaatttat taatcaatcg gatgtggcgt
     7681 tccatttacc tatacgcaga cataattcaa ataagtacga tttcggcaaa ctactcatta
     7741 tcgccggttc acggggcatg tcgggagctg ctttgatgac agcgcgcgcc gcaatgcgat
     7801 caggtgccgg tgttgtgcgg gttgcgatac ccaaatcaat agcgcacgtg atcgaacaag
     7861 gtttgcctga agccatgacc gtacacttac cggagacgga ggatggcgta ataagcc
//
LOCUS       DAOQGF010000134         7834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4435_length_7834_cov_6.779792, whole genome shotgun sequence.
ACCESSION   DAOQGF010000134 DAOQGF010000000
VERSION     DAOQGF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4435_length_7834_cov_6.779792"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1536
                     /locus_tag="PK937_13805"
     CDS             256..1536
                     /locus_tag="PK937_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNH33697.1"
                     /translation="MKNILIPTAIITTILLLLSNSSLHGQTVSSAAMSKKHVLGKWVQ
                     LPDTLTFCGEVIPLNDPDVRERAEDIFYTRLGDDDRFFLLLRRTSRYFPFYEKIFREM
                     ELPSDLKYLSVAESSLRIDAYSSADATGLWQFIAGTAKIWGLRVDKYVDERFHVEKST
                     RAACALLKNLRMTFGSWSLAAAAYNTGEANIEKAVDGQHDDNYYNLFLNKETRQYVIH
                     IAVLKEILEHPEKYGYAIPDSTYFKPFDDRTETITLKGPVKDLGLWAKSIGTNYKTIK
                     MLNFWIMRYDLPEGIWELRVPEGLSRDSSLMQFWQSKNDSIIYGENNRVTIFHTVQPG
                     ESQIRIAAMYSVSIQELKDWNGIKNDAVVVNQKLKLKIPAHTKIQYPVRSGDNLLKIA
                     EQFKVKAERIKEWNKRELNGLQAGEVLTIYIGKN"
     gene            complement(1537..2421)
                     /locus_tag="PK937_13810"
     CDS             complement(1537..2421)
                     /locus_tag="PK937_13810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNH33698.1"
                     /translation="MRELMVSRNRLTLFLAALVSFLFISMSILGFLFSNYSGDERLAE
                     LNAENRILKDQLSDIGGRMKVIDEQLKLIRTKDSELRVIADLPDINMLLKDAGIGGSD
                     LEIDYNPDLLSDDADALVRKNLENLEKLESNIKLELQSYAELHENISANAAKLVYLPS
                     INPIKRGRITDRFGVRKSFRSFIPHTGLDIAARWGTPVYVTADGVVEGVTWRGGYGKS
                     IIVDHGNGIKTLYGHLSSYKVKNGERVKRNQKIAEVGSTGLSTGPHLHYEVRINNVPQ
                     NPELFIFFDMVDYLEMQW"
     gene            2676..4595
                     /gene="speA"
                     /locus_tag="PK937_13815"
     CDS             2676..4595
                     /gene="speA"
                     /locus_tag="PK937_13815"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNH33699.1"
                     /translation="MTWTTDDANKIYNVEEWGAGYFHINAKGHISVTPTGDLGKSIDL
                     KTVVDDIKARGIALPVLVRFQDILRSRVRELNEHFRRAISEYNYQGKYHGVFPIKVNQ
                     LREAVEEILDAGIPYGYGIECGSKPELYVALALVEDKETLIIANGYKDAQFIRMALLG
                     KQLRKNVIIVVEKLSELRQILQISKEIGVEPAIGIRAKLSTKGTGKWEASSGDQSKFG
                     LSMPEIVEAIEMLKENNLTSAFKLLHFHMGSQIPDIRTIKAAAKEASRIYASAYKMGM
                     PIQFIDVGGGLGVDYDGSRTTFRSSMNYSLQEYANDIVYTILEVCNSEEIPHPNIVSE
                     SGRAIVAHHSVLVLEIFGNIEKKPSAFKMDFSPEDPDIIQEMLDLRHQLHKKNTNYLE
                     VYHDAAQRREEAHSLFSLGYLTLIEKAKIENLYWEIINIIREYSRSLEVIPEELAELE
                     PKLADQYLCNFSIFQSLLDHWAIDHLFPIVPIHRLNEEPTKRAILVDITCDSDGKICK
                     FIDMKDVKDKLELHPLNDDPYYIGIFLLGAYQDIMGDMHNLFGRVNEVHVFMDDEEPD
                     NYYVEEIIPGSNVGQVLSLVQYNENEIIKLIKKTVDQRIKEGQLKPSEGVKLLDTYEA
                     GLKDYTYLQLNGRSR"
     gene            4616..6163
                     /gene="lnt"
                     /locus_tag="PK937_13820"
     CDS             4616..6163
                     /gene="lnt"
                     /locus_tag="PK937_13820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNH33700.1"
                     /translation="MITRTRILYLTGPLLFLLSFPPLPTGFLAYIALLPFFELLRQNQ
                     YRSGFRTGYGLGLLTMGVFIHWLNANTGATLPQATGMYFGTIMYLALYWGCFGAIQGF
                     IQRKLGERGLWLAPFLWASLDYVQSLGELGFTWHNLATTQSYYLPLIQFIDITGIYGL
                     SFWIALVNVIIFKLINAYREQQKNQAVSYAVGLGMIFLLPLVYGFLSSWRETSEKKLR
                     VAIIQPNIEPNRKWLEHDFAYEELLRLTQSIPEGKTDLIIWPETAIPVRLRQDRTKLA
                     AIREILKEKKASLLTGIPDRKMTESEDGRRSAEYYNAALVIRPDNTQLGNYEKVHLVP
                     FGEFVPSFLKFLDAIAMEVGASNYTPGDSVHAFTIPLYSDSIPTDSACITAVICLESI
                     FPYLVREGLERDAQALVIVTNDSWYDGTMAPEQHARIAVLRAIEFRTAVARCANSGVS
                     NAIDPYGRITAKLDNGLQGVLYATIPLQTSTSIYMRWGDWFAHSLWMIITLSLCFLFF
                     YKPSKRD"
     gene            6166..6903
                     /locus_tag="PK937_13825"
     CDS             6166..6903
                     /locus_tag="PK937_13825"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNH33701.1"
                     /translation="MIQKPSFFISDIHFMGGRSAEELHKYRMVKSFFEHVAEYGQELF
                     ILGDYFDFGIDYRHSLPSQNVRGLALLVLLADAGVQIHYLVGNHDFWLGDFLQKELDI
                     KLYYEPFSISRNGKNIYLIHGDGLSDLDKSYRAMRRILRARFNQFLFRWLHPDLGIAL
                     ANRMSHASKNQDVLYTKYNQDESYFKPLSNIFDQGYDMILMGHHHQPMMRVFNNKIYY
                     NLGDWIQHGSYLESDAQGFSQKTWKLT"
     gene            6900..>7834
                     /locus_tag="PK937_13830"
     CDS             6900..>7834
                     /locus_tag="PK937_13830"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNH33702.1"
                     /translation="MSNIRRVLFILLCFQAPLFAQAQVSTDALKSQLTSLRELLRDTA
                     RFRSVHAPVLMDQLMDWNEITRRKFEANWVNLTKSWQLRFERAIRQRLSEVFTQYLIH
                     NAATLRGAESRWFDEEIGITRAKASLEIVQNQEIVRIALRLSFSENRWRIYDIRTPEF
                     RLLRDLLPDCDEKISDGYSNEYVEALITNAEHFVIDDFNAVESGVFPRNWGWRKRDDD
                     LMRSSQRVYSVVKDSQDSYLSAIARGTSVALVRPYSYDLREYPVLTWKWRVNTMPSAS
                     SQNDAIENAASVTVIFYQNWLGVPITITYAWAYEGG"
BASE COUNT         2171 a         1772 c         1715 g         2176 t
ORIGIN      
        1 aaaccatgcc tactaaaaaa gctggtgcga agaaacccgc tgctaaaaaa gccgcgaaga
       61 agaccgctaa gaaaacgacc aaaaaagctg ctaagaagaa gtaatctctt ctaatcgctt
      121 acttaccggg ttccgactgg attacacccc gtaagttgtc gtttgcggag ggttacacga
      181 cctccacagt ctttgaaaaa gcaggtatcc ttgatgcctg cttttttctt ttcttttttt
      241 taattatgat ttgctatgaa aaatatttta atacctaccg ctatcataac tacaatccta
      301 ttgctgttaa gcaactcatc gctgcacggt caaaccgtga gctccgctgc aatgagtaaa
      361 aaacatgttt tgggtaaatg ggtacaactt cccgatacgc tgactttttg cggagaggtc
      421 atccctctca atgatcccga cgtacgcgaa cgtgctgaag atatttttta tacacggctg
      481 ggtgacgatg atcgcttctt tcttttatta cgacgcacct ctcgttattt cccgttttat
      541 gaaaaaattt ttcgtgaaat ggaattacca tccgacctta aatacctatc tgtcgccgaa
      601 agctcgttgc gcattgatgc atactcttca gccgatgcca cagggttatg gcaatttatt
      661 gcaggaacgg ccaaaatttg gggattgcgc gtagataaat atgtcgacga acgatttcat
      721 gtcgaaaaat caactcgtgc tgcatgcgct cttttgaaaa atctgcgcat gactttcggc
      781 agttggagtt tggccgccgc agcatacaat acaggtgaag caaatatcga aaaagccgtc
      841 gatggccagc atgatgataa ctattacaat ctttttttaa acaaggaaac acggcagtac
      901 gtgattcaca tcgccgtact caaggaaatt ttagaacatc ctgagaaata tggttacgct
      961 attccggact ccacctactt caaaccattc gatgatcgta cggaaacaat aacactaaaa
     1021 ggtccagtca aagatttggg tttgtgggca aaatcaatcg gtaccaatta caaaacgatc
     1081 aaaatgctta acttctggat tatgcgctat gatttgcctg aaggcatttg ggagctacgc
     1141 gttcctgaag gcctctcacg tgattcttcg ctcatgcagt tttggcaatc aaaaaatgat
     1201 tcgatcattt acggcgaaaa caatcgggtg acgatttttc acacagttca accgggtgaa
     1261 tcgcagatac gtattgccgc aatgtatagc gtaagtattc aggaactgaa agattggaac
     1321 gggattaaga atgatgctgt tgtggtgaat caaaaattga aattgaaaat ccctgctcac
     1381 actaagatac aatacccggt tcgcagcggt gataatcttt tgaaaatcgc cgaacaattt
     1441 aaagtcaaag cggaacgcat caaagaatgg aataaacgag aattgaatgg tttgcaggca
     1501 ggcgaagtac ttacgatcta tatcggaaag aattaattac cattgcattt ccaaataatc
     1561 caccatatca aagaaaataa aaagctctgg gttttgaggt acattattga tgcgcacttc
     1621 gtagtgcaaa tgcggcccgg tggataaacc agtgctcccc acttcagcaa ttttttgatt
     1681 acgctttacc cgttccccgt tttttacttt atacgatgac aaatggccgt atagtgtttt
     1741 tattccgtta ccgtgatcta cgatgataga tttaccgtaa ccaccgcgcc aagtcacccc
     1801 ttcgacaacg ccatcggccg taacataaac cggcgttccc caacgtgcgg caatatccaa
     1861 accggtgtgc ggtataaacg aacgaaatga tttacgaacg ccgaaacgat ctgtgatgcg
     1921 cccgcgtttg atcggattga ttgacggaag ataaaccaac tttgctgcat tggcgctgat
     1981 gttttcatgc agttccgcat aactctgcaa ttcgagttta atattacttt ccagcttctc
     2041 taagttctca agatttttac gcaccaacgc atccgcatcg tccgataaca aatccgggtt
     2101 gtaatcaatt tccaaatcac tgccaccgat gccggcatct ttaagcagca tattgatatc
     2161 cggtagatcg gcgatgactc gcagttcact atccttagta cgaatcagct taagctgctc
     2221 gtcaatcact ttcatacggc cgccgatatc ggaaagttga tctttgagaa ttcgattttc
     2281 tgcgttgagt tcggccaggc gttcatcacc ggaataattg gaaaacaaaa aacctagaat
     2341 actcattgaa ataaataaaa aactaaccaa agccgccaaa aacaaagtaa ggcgattccg
     2401 ggaaaccata agttcgcgca tacccgattg attctcgggg acgtataaca agcggaaccg
     2461 tttgtcagta aaccatttca tgggcataaa agtacataag catgctctta aaggcaagat
     2521 gttttgagga taccgcctgc actttttcca aaaaaagctg cggtgcaaag acatttacta
     2581 ttgcatatct atgcggttcg gtataatatt gcgcaccatt aattttaact ttacaacgca
     2641 gactgaaata cctgcatttt gaggagacgc cgaagttgac ctggacaacc gatgatgcca
     2701 ataaaatcta caatgtcgaa gaatggggtg cgggttattt tcacatcaac gccaaaggcc
     2761 atattagcgt aacgcccacc ggcgatcttg gcaaatccat tgaccttaaa acggttgtag
     2821 acgatatcaa agcacgcggc atagccctgc ccgttcttgt tcggtttcag gatattcttc
     2881 gaagccgcgt tcgtgagctc aatgaacatt ttcgccgtgc catcagtgaa tacaattatc
     2941 aaggcaaata ccacggcgtt ttcccgatca aagtcaatca actgcgtgag gccgtcgaag
     3001 aaatactgga tgccggtatt ccctacggtt acggtattga gtgtggcagc aaaccggaac
     3061 tgtatgtcgc gctagctctt gtcgaagata aagaaacgct catcatagcc aacggctata
     3121 aagatgcgca attcattcgt atggcgctcc tgggtaaaca actccgaaag aacgtcatca
     3181 tcgttgtcga aaagttatcc gaactgcgac agattttgca aatatccaaa gaaatcggtg
     3241 tcgaaccggc aatcggtatt cgcgctaaac tcagtaccaa aggcaccggc aaatgggaag
     3301 cctccagcgg tgatcagtct aagtttggcc tttctatgcc ggagatcgtc gaagcgatcg
     3361 aaatgctcaa agaaaataac ctgacgagcg cttttaagtt actgcacttt cacatgggtt
     3421 cccaaatacc cgatatccgc acgatcaaag cagcggctaa ggaagcttcg cgcatctatg
     3481 cgagcgctta taaaatgggc atgcctattc aatttattga tgtcggtggc ggtttgggcg
     3541 ttgactatga cggatctcgg acaacatttc gcagcagtat gaattatagt ttgcaggaat
     3601 atgccaacga tatcgtttat actattttgg aagtatgtaa ttcggaagaa atcccccatc
     3661 ccaatattgt cagcgaaagt gggcgcgcca tcgtagccca tcattccgtt ttggttttgg
     3721 aaatttttgg taatattgag aaaaaaccgt ccgcatttaa aatggatttt tctcccgaag
     3781 atccggacat tatacaagaa atgctggatc tgcgccatca actgcacaaa aaaaacacca
     3841 actatctcga agtctatcac gatgccgcac agcgtcgcga agaagcgcac tcgttattct
     3901 cactcggtta tctgacgctt atcgaaaaag ctaaaatcga gaacctgtat tgggaaatca
     3961 tcaatatcat ccgtgaatat tcccgcagtc tggaagtgat acccgaagaa ttggccgaac
     4021 ttgagcccaa attggccgat cagtatctct gcaatttttc gattttccaa tcgttgctgg
     4081 accattgggc gattgaccat ttattcccca ttgtaccgat acaccgactc aacgaagagc
     4141 cgaccaaacg cgctattcta gtggacatca cctgtgactc tgacggaaaa atctgtaaat
     4201 tcattgacat gaaagatgtc aaagataaac tggaactcca tccgctgaat gatgatccct
     4261 attatatcgg tattttctta ttgggcgcct atcaggatat catgggtgat atgcacaatc
     4321 ttttcggccg cgtcaatgaa gtacacgttt ttatggatga cgaagaaccg gataactact
     4381 acgtcgaaga aatcattccc ggttccaacg ttgggcaagt attatcgctg gtgcagtaca
     4441 atgaaaatga gatcatcaaa ctcatcaaaa aaaccgtgga tcagcgcatc aaagaagggc
     4501 aactcaaacc cagcgaaggt gttaaattat tagacaccta cgaagccgga cttaaagatt
     4561 atacctatct tcagctcaac ggccgttcac gttagactgt ggcggatata ggactatgat
     4621 tacacgcacc cgcattttgt atcttacggg gccgttgctt tttttattaa gttttccccc
     4681 gcttcctacc ggatttttag cctatatcgc tctccttcct ttttttgaat tgctgcgcca
     4741 aaaccaatac cggtccggtt ttagaaccgg ttacggcttg ggtttgctta ccatgggtgt
     4801 atttattcat tggctcaatg ccaataccgg agcaacacta ccgcaggcca ccggcatgta
     4861 tttcgggacg atcatgtatc tggctttgta ctggggatgt ttcggcgcca tacaaggttt
     4921 tatccaacgc aaacttggtg aacgtggttt atggttagct ccctttttgt gggcatcgct
     4981 ggactatgtc cagtcgctcg gcgaacttgg ttttacttgg cataatctgg cgacaaccca
     5041 gtcttattat cttcctttga ttcagtttat agatattacc ggcatttacg ggctttcgtt
     5101 ttggatcgct ttggttaatg tcatcatttt caaactaatc aatgcctatc gtgaacaaca
     5161 aaaaaatcaa gcggtgtcct atgctgtggg tctgggaatg atttttcttt tgccgctagt
     5221 ctatggcttt ttgtcgtcgt ggcgggaaac ctcggaaaaa aagttacgtg tagcgattat
     5281 acaacccaat attgaaccga atcgcaaatg gcttgagcat gattttgctt acgaagaatt
     5341 gttgcgttta acgcaatcta ttcctgaagg caaaaccgat ctgatcattt ggccggaaac
     5401 ggccattcct gttcgattga gacaagatcg aactaaactc gcagcgatac gtgaaatctt
     5461 aaaagaaaaa aaagcctctc tgctcaccgg aatacccgat cggaaaatga cagaatcgga
     5521 agacggacga cggtcggctg aatattataa cgccgcactt gttattcgcc cggataatac
     5581 gcagcttgga aattatgaaa aagttcatct tgttcctttc ggcgaattcg ttccgagttt
     5641 tttgaagttt ttggacgcca tcgccatgga agtcggtgcc tctaactata cgcccggcga
     5701 ttcagttcat gcttttacca ttcctctcta ttccgatagc atcccgaccg actccgcgtg
     5761 tattaccgct gtcatttgcc ttgagtccat attcccttat ctagttcggg aaggtttgga
     5821 gcgggatgcg caagctttgg ttattgtaac caatgattca tggtatgatg gtacgatggc
     5881 gcctgaacaa cacgcacgca tcgccgttct gagggcgatt gagtttcgca ctgcggtagc
     5941 ccggtgcgcc aactccggcg tctccaatgc cattgatccc tacggacgca ttacggctaa
     6001 gttggataat ggcttgcagg gagtgctgta tgcgacgata ccgttgcaaa ccagtaccag
     6061 catttatatg cgatggggcg attggttcgc tcacagcctt tggatgatca tcactttatc
     6121 gctgtgcttt ttgttttttt ataaaccgtc gaagcgcgat tgaccatgat tcaaaagcct
     6181 tcatttttca tttccgatat tcattttatg ggcgggcgct cagcagagga acttcataaa
     6241 tacagaatgg tcaaatcttt ttttgagcac gtcgctgaat atggacaaga gctttttata
     6301 ctcggggatt attttgattt tgggattgat taccgccaca gccttccttc gcaaaacgtt
     6361 cgcggccttg cgttgctggt attattggca gacgcaggag tacaaattca ttatcttgtc
     6421 ggcaatcatg atttttggct cggtgatttt ttgcaaaagg aattggatat taaactttat
     6481 tatgagccat tttcaatttc acgtaacggg aaaaatattt atcttataca cggtgacgga
     6541 ttatccgatc ttgataaatc ctatcgtgcg atgcgccgca ttttgcgcgc tcgtttcaat
     6601 cagtttttat ttcgatggtt acatcccgat ctcggtatcg ccttagccaa tcgtatgtcg
     6661 cacgcaagca aaaaccagga tgttttatat accaaataca atcaggatga aagctatttt
     6721 aagccgttga gtaacatttt tgatcagggc tatgatatga tattgatggg acatcatcat
     6781 cagcctatga tgcgtgtttt caacaataaa atatattata atttgggaga ctggattcag
     6841 cacggcagtt accttgaatc cgacgcgcag gggttttcgc aaaaaacttg gaaacttaca
     6901 tgagtaatat tcgacgagta ttgtttatcc tgttatgctt tcaagcgccg ctctttgcac
     6961 aagcacaggt ttcaacggac gcattgaagt cgcagttaac ttccctacgc gagctattaa
     7021 gagatacggc gcgcttccgt tcggtgcacg ctccggtttt gatggaccag ttgatggatt
     7081 ggaatgaaat tacacgtcga aaatttgaag ccaattgggt gaaccttacc aaaagctggc
     7141 agttacgttt cgaaagagcg atacgacagc gtcttagcga ggtttttacg cagtacctga
     7201 tacacaacgc agcaacgctg cgcggtgccg agtcccgttg gtttgacgaa gagatcggta
     7261 taacgcgtgc caaagcgtct ctggaaatcg tacaaaatca agagatcgta cgcattgcat
     7321 tgcggctttc tttttccgag aaccgatggc gaatatacga tatacgcacg cccgaatttc
     7381 gtttgctgcg tgacctttta ccggattgcg atgaaaagat cagtgacgga tatagcaatg
     7441 aatacgtaga agcgctgatc accaacgccg aacattttgt cattgatgat tttaacgccg
     7501 ttgagtctgg cgtttttccg cgcaattggg gttggcgcaa acgggacgat gatctgatgc
     7561 gctcttcaca gcgcgtctat agtgtcgtca aagacagtca ggacagctat ttatccgcca
     7621 tagcccgcgg cacatccgtt gcgctagttc ggccttacag ttatgatctc cgtgaatatc
     7681 ctgtactcac atggaaatgg cgcgtcaata ccatgccatc ggcttcatct caaaacgatg
     7741 cgatcgaaaa cgccgcctcg gtaaccgtca ttttttatca aaattggctc ggggtaccga
     7801 tcaccataac ctatgcctgg gcctatgaag gcgg
//
LOCUS       DAOQGF010000135         7776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4498_length_7776_cov_7.617666, whole genome shotgun sequence.
ACCESSION   DAOQGF010000135 DAOQGF010000000
VERSION     DAOQGF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4498_length_7776_cov_7.617666"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2991
                     /locus_tag="PK937_13835"
     CDS             <1..2991
                     /locus_tag="PK937_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNH33703.1"
                     /translation="AQLAQNWSLAFSSASHAIGVNPLNRSIVYIYNTSDSKFYVSYDY
                     GLSWIPRGILNAGGDIRNITVNPLDTNVVIAVGFGIFKSNDGGKTWREALANVTIDGE
                     SIDYNYLHPDTMYFVDFSMRKFYLSADTGSSWVERSTVGFGPICTVVSHPQNPNIIII
                     GSGDTRIERSTDMGLTWQLVKQGNPYFSEIPKMAWDKSDPNFIYGSTYVDNQYSFFRS
                     TNGGLTWEDAGIYGMYTWGLDYGIDRGDVFIGTFGYDGADRGIYRSVDRGQSFMKVGT
                     ANLGDTFLNTWMIKTAADGNVFFVNENKVYRTQFGVEGKVTGVVRTQNNTPLRFASIF
                     VDETGDSLFIGNINGNYTLALPPGQYTLRARVAGVESTPVQVTITADNTITQDFQVPI
                     SETFHTVSGTVTAIDGEPINVNIQLHGRYGNGSRFTKETSTTSAFSFTNLSSLSLYDS
                     IVVIPQELPYITSIIKPLTLDASVPVSIDIADVLIVSETYVPSGAYLEYYTNRLNEAG
                     YKATGLEGYSLSSIPEHALLKTKYKTALWLTRRIEMDMDPLWHDTLVSFIQKGGHVMI
                     AGQDILEKNSSLPVFSEYLKLGFGGNYTTDPTIQGIDGNLIGNGLNFSISQSTLPSRD
                     RISFSNANASVAFRYGTLPEDADKYAGVYISNTGFGGKAVVLGFDPYRVGDLALRTIF
                     DRTLKYFNGEEGAFDLTVSVFQNSLIRNRGSIVIAATHILQIPPIVKIAKGTDTTQVT
                     VTALSGAKVYSGAFNLSEGSYKLITRGVSHKGPDSTKLRSFNVSLLKPQTEALITVPD
                     ASATLAIEKQNFTGYVMATTEDKGAEKIFHFGTPDMTALARIAIKVPGISVETAEKTL
                     IYRQEGNEWKWVPTVFVPGQDVLEASVERFGTYKTVYDPQTSASTEILRSFELKQNYP
                     NPFNPTTTITYDIPSTMKVSLVVYNILGQKIKTLWDGVRTAGRYTQIWNGRNEKGQLV
                     TSGVYLYQIKAGNKTSVKRMLLIK"
     gene            complement(3082..3567)
                     /locus_tag="PK937_13840"
     CDS             complement(3082..3567)
                     /locus_tag="PK937_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH33704.1"
                     /translation="MKEFLEDFEETVKKSVKRLSKLSDQESGIAKTPDKWSAKQIIGH
                     LIDSAANNHQRFVRAQFGDDLVFPGYDQEKWVKVQRYDLSDWNALINLWNSYNFHIIH
                     VVKQIPESDLFKSRSKHNLDQIAWKTVPVSESVTLDYFIRDYVGHLKNHLDQIWSQYK
                     V"
     gene            complement(3734..6154)
                     /locus_tag="PK937_13845"
     CDS             complement(3734..6154)
                     /locus_tag="PK937_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNH33705.1"
                     /translation="MRYLFVFFTFLLSMSAAYGQYTEYPTARYYHEKEITPREHTINI
                     ERMKLDVQFDPPKGLVKGKVTHVFKPLRASLDQLYFDGVNLRVTDVTINGKTAQFVNS
                     GQGITVFAKPALTWNDIRDSITFTYEANPRKGIYFVGWNDSTRRMRRQIWTQGEERDH
                     RYWIPMYDDPNDKVIHETSITFDKTYRVIANGKLQKEKDNKDGTKSWTYAMSQPHAPY
                     LLMLAIGQYEVKNLKSKSGVPIQLWYYPDQADRIEPTYRHTVTAMDFLEKEIGIPYPW
                     GVFANIPVSDFVTGAMENTTAVLFGDFWYVDRRAFNDDNYVDTDFHEQAHQWFGDMIT
                     MYDWKHLWLQESFATYYGKLGMYHVYGEDMYQWKRREEQNQAINASENNRLPITHTQA
                     GTARFYPKGSAVIDMMNHVWGRDAVRRAVYHFLSHNRFKNVETYGLMRSFMDTLGVAP
                     QWFFDQWIYRGGEPHYKISYLDLRRTSGRQTEITVQQTHKTDELTGLFKMPIDFEVHY
                     ADGTKDSVREWIENTVTTVVVPNTHDKEIAFVLFDPGSWVTKSYEFEKHFDELAAQAL
                     QAPNMIDRYDALVMLRAYDLPSKRELLIRVYDKETFHALKAEVAEQLVNDEDEAGRAV
                     IRRAITDAWPEVRKAVINNVGRIPQTMLSDFEKLLSDSSYNVVRVALARLINQYPVHA
                     DRYLDMTKDQYGLSNEIKMDWLEQKVNRGDKDALMQIVEYSSNAYEFGTRRNAMSALK
                     RLNYLDDKVIGNLLDAALNANESLSNRAKNILDYFTDQSPYRRRIQSYYRSHTWEDWQ
                     KEILQALL"
     gene            complement(6324..7598)
                     /locus_tag="PK937_13850"
     CDS             complement(6324..7598)
                     /locus_tag="PK937_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNH33706.1"
                     /translation="MHRHESIFALIDCNNFYVSCERAFQPKLEGKPVVVLSNNDGCIV
                     SRSNEAKALGVPMAAPYFEVKGLLDKHHAHVFSSNYVLYADMSHRVMQVLQRFTPDVE
                     VYSIDEAFIDLSGFTEDLTTYGIKIKETISRWTGIPVSVGIARTKTLAKIAAEKAKKS
                     KKLKGAFSLVNSPYLEKTLELTEVGDVWGLGRSSTAHLQSHGIYNALQFSKANPAWIK
                     KTMSIVGLRIAQELREVSCLPLAQVRERKKGITTSRSFSRAVTSLEEMTEAMATYVDR
                     CAKKLREEGAATKTLTVYLMTNRFTDDHYYYHKVVTLPVPSDDTGELIAYATSALRSI
                     FKEGLRYKKCGVMFTELIPKETVQTDMFDRISRERSSRRMEVLDAINARMGNGTLRYA
                     SEGWKKDWHMKCENRTPEYTTQWDQLLTVSAE"
BASE COUNT         2053 a         1801 c         1729 g         2193 t
ORIGIN      
        1 gctcagcttg cacaaaactg gtctttggcg ttttcctctg caagccatgc catcggcgtc
       61 aatccgctga atcgcagtat agtgtatata tacaatacct ctgacagcaa attttatgta
      121 tcgtacgact atggcttgtc gtggattcct cgcggcatct taaacgcggg tggcgacata
      181 cgcaatatca cggtcaatcc gctggacacc aatgtcgtca tagccgtcgg atttggtatt
      241 tttaaaagta atgacggggg taagacatgg cgtgaagcct tagccaatgt aactattgac
      301 ggagaatcca ttgattataa ttatctccat cccgatacga tgtattttgt agatttcagt
      361 atgcggaaat tctacctcag tgccgatacc ggttcgtcgt gggttgaacg ttctactgtc
      421 ggattcgggc cgatctgtac cgtcgtatcg catccacaga atcccaatat tatcattatc
      481 ggttccggcg atacacgcat cgaacgctcc accgatatgg ggctcacctg gcaactcgta
      541 aaacaaggca atccgtactt ttcggaaatc cccaaaatgg catgggataa aagcgatcct
      601 aattttatct acggaagtac atatgtggac aatcagtatt ctttttttcg aagtaccaac
      661 ggcggattga cttgggaaga tgccggcatt tacggaatgt acacctgggg actcgactac
      721 ggtattgatc gcggtgacgt ttttatcggc acattcggtt atgatggtgc ggatcgcggc
      781 atctatcgca gtgtagatcg cggtcaaagt tttatgaaag tgggaacggc taatctcggt
      841 gatacttttt taaatacatg gatgatcaaa acggccgcgg acggtaatgt tttttttgtc
      901 aatgagaata aagtatatcg aacccaattt ggtgtagaag gaaaagtcac cggagtcgtg
      961 cgcacacaaa ataatacacc gcttcgtttt gcttccattt ttgtggatga aaccggagat
     1021 tcgctgttta tcggaaatat aaacggtaat tacacattag cgctgcctcc gggccaatac
     1081 acactgcgag cgcgtgtcgc aggtgtagag agtacgccgg tacaagtaac gatcacagcc
     1141 gataatacga tcactcaaga ctttcaagta ccgatttctg aaacatttca taccgttagt
     1201 ggcacagtaa cagctattga cggtgaaccc atcaacgtca atatccaact tcatgggcgt
     1261 tatggtaatg ggtctcgttt taccaaagaa acttcaacca cttcggcttt ttcgtttaca
     1321 aatctgtcat cattatcact atatgattcg attgttgtaa taccgcaaga actgccgtat
     1381 atcacctcaa tcatcaaacc gttaactttg gatgcatccg ttccggttag catagatatt
     1441 gcagatgtcc tgatcgttag tgagacgtat gtcccaagtg gtgcgtacct cgaatattat
     1501 accaaccggt taaacgaagc gggttataaa gcgacgggat tggaaggata ttcgttgagt
     1561 tctatccccg aacacgccct tttgaaaaca aaatataaaa cggcgttatg gctgacacgg
     1621 cgcatagaaa tggatatgga tccgctatgg cacgatacgc tggtatcatt tatccaaaaa
     1681 ggcggtcatg tgatgatcgc tggtcaggat attttggaga aaaatagttc gttacctgta
     1741 ttttccgaat atctgaaatt aggttttgga ggaaactaca cgaccgatcc tactattcag
     1801 ggcattgatg gtaaccttat aggcaatggt ttgaattttt ccatttctca atccacgttg
     1861 ccttcacgcg accgaatcag tttttccaat gccaatgcaa gcgtcgcttt ccgctacggt
     1921 accttgcccg aagatgccga caaatatgcg ggcgtttata tttctaatac gggttttggg
     1981 ggaaaagccg tcgtactggg gttcgatcca taccgtgtcg gcgacttggc attgcgaacg
     2041 atatttgatc gtacgctcaa atattttaat ggtgaagagg gggcgtttga tttgacggtt
     2101 tcagtattcc aaaattcgct gatccgtaat cgcggcagta tcgtcatagc agccacacat
     2161 attttacaaa taccaccgat cgtcaaaatc gccaaaggta cggatacgac acaagtcacg
     2221 gtgaccgctt tgagcggtgc taaagtatat agcggcgctt ttaatctatc cgaaggtagt
     2281 tataaactca ttacacgtgg tgtgtctcat aaaggacccg attctacaaa actgcgatcc
     2341 tttaatgtct ctttactcaa accgcaaaca gaagctctga ttacagttcc tgatgcttcc
     2401 gcaacattgg ctatcgaaaa acaaaacttt accggttacg tcatggcgac aacagaagat
     2461 aagggtgcgg aaaaaatatt tcattttggt acacccgaca tgaccgcact ggcgcgaatt
     2521 gccattaaag ttcccggcat ctccgttgaa accgctgaaa aaacgttgat ctatcgtcag
     2581 gagggaaacg aatggaaatg ggttcctacg gtatttgtac cgggccaaga tgttttggaa
     2641 gcgtccgtcg aacgattcgg tacatacaag accgtatacg atccgcagac gtcggcatca
     2701 acggaaattc ttcgttcatt tgaacttaag caaaattatc ccaatccgtt taacccgacg
     2761 actacgatca cgtacgacat cccatccacg atgaaagttt cattggtggt ttataatatc
     2821 ctcggtcaaa aaataaaaac gttatgggat ggtgtacgta cggccggacg ttatacacaa
     2881 atatggaacg gtcgtaatga aaagggtcag cttgtaacaa gcggagtgta tttgtatcaa
     2941 atcaaggccg gaaataaaac cagcgtcaaa cgtatgcttt tgatcaaata attcatgcga
     3001 gagatccgta tttctgtccg tcaaatgtca aatcaactat caggttttca tacagagaat
     3061 actcgccggc tgatctgatg tttacacctt gtattgcgac caaatctgat caagatgatt
     3121 tttcaaatgc cctacataat ctctaataaa ataatcgagg gtgacggatt cacttaccgg
     3181 cactgttttc caagctattt gatcaaggtt atgttttgac cggctcttga aaaggtcaga
     3241 ttccggtatc tgtttcacca catgtatgat atgaaagttg taagaattcc aaagattaat
     3301 caacgcattc caatccgaca gatcataccg ctgaactttc acccattttt cctgatcgta
     3361 acccggaaat actaagtcat caccgaattg ggctcgtaca aaacgttgat gattattagc
     3421 agcagaatca atcaggtgtc caataatctg tttggccgac catttatcgg gcgttttggc
     3481 aataccgctt tcttgatcgc tcagttttga aagacgtttg accgattttt tcacggtctc
     3541 ttcaaaatct tcaagaaact ccttcatttt ggatattcta ctccgatgat ttgatgcgca
     3601 tttacgcgtg cagttcccga tcaaaaaata aattcgaaac ctgacacgtt atgatcgtac
     3661 tattcatgag ttcgatttat gaacttttct tcacactcat tgcccatcaa agcgaccgcg
     3721 ccaatgatca catttacaac aacgcttgta aaatctcctt ttgccagtct tcccatgtat
     3781 gtgaacggta gtaggattgg atgcggcgac gatacgggct ctgatccgta aaataatcca
     3841 aaatattttt cgcacggtta ctcagcgatt cgttggcatt gagcgcagca tccaaaagat
     3901 ttccgatcac tttatcgtct aaataattga gccgcttgag cgcactcatc gcattgcgcc
     3961 gcgtaccaaa ttcgtatgca ttgctgctgt attccacgat ctgcatcaac gcatctttat
     4021 cgccgcgatt gactttttgc tccagccaat ccattttgat ttcgttggat aacccgtatt
     4081 gatctttggt catatccaga taacgatcgg catggaccgg atattgattg atcaggcgtg
     4141 ccagcgcgac acgtacgacg ttgtacgaac tgtccgacaa tagtttttca aaatcggaaa
     4201 gcatggtttg cggaatgcga ccgacattat tgatcaccgc cttacgcact tccggccaag
     4261 catcggtgat cgcgcgacga ataaccgctc tgccggcttc atcttcatca ttgactaact
     4321 gttcggcgac ttcggctttg agtgcgtgaa atgtttcttt atcgtaaacg cgtatcagga
     4381 gttcgcgttt ggatggcaga tcgtaggcac gtagcatgac gagcgcatcg taacgatcga
     4441 tcatattagg cgcctgtagt gcctgcgccg ccagttcatc aaaatgtttt tcaaattcat
     4501 aggacttggt aacccaactg cccggatcaa aaagcacaaa ggcgatctct ttatcatggg
     4561 tattgggtac tacgacggtg gtcaccgtgt tctcaatcca ttcacgcacg ctatctttgg
     4621 taccgtcagc atagtgtact tcaaaatcaa tcggcatttt aaaaagcccc gtcagctcat
     4681 cggtcttatg ggtttgttgc acggtgattt ccgtttgacg gcccgaagta cggcgcagat
     4741 caagataact gattttgtaa tgcggctcac cgccgcgata aatccattga tcaaaaaacc
     4801 actgcggcgc cacgcccagc gtatccataa aggaacgcat caggccgtag gtttccacat
     4861 ttttaaaacg gttgtggctt aaaaaatgat atacggcgcg gcgtacggca tcacgacccc
     4921 agacatgatt catcatatct atgacggccg accctttggg ataaaagcgt gccgtgccgg
     4981 cttgcgtatg cgtgatcgga agacgattgt tttccgatgc gttgatcgcc tgattttgtt
     5041 cttcacggcg tttccactga tacatgtctt cgccgtacac gtgatacata ccgagtttgc
     5101 cgtagtacgt cgcaaaactt tcctgaagcc aaagatgttt ccagtcatac atcgtgatca
     5161 tatcgccaaa ccactgatgc gcctgttcat gaaaatcggt atctacgtag ttatcatcat
     5221 taaaggcacg acggtccaca taccaaaaat caccgaataa aacagccgtt gtattttcca
     5281 tcgcacccgt cacgaagtcg gaaacgggta tattggcaaa aacaccccag ggataaggta
     5341 tcccgatttc tttttccaaa aaatccatcg ccgtaacggt atggcggtac gtcggctcga
     5401 tacgatcggc ctgatcggga taataccaca actgaatcgg aacgccgctt ttggatttga
     5461 ggtttttgac ttcatattgt ccgatcgcca gcatcagcaa atacggcgca tgcggttggc
     5521 tcatggcata cgtccaactc ttggtaccgt ctttgttgtc tttttctttt tgcagtttac
     5581 cgttagcgat gacgcggtag gttttatcaa acgtgatcga agtctcgtga atcactttat
     5641 cgttggggtc gtcatacatc ggtatccagt agcgatgatc gcgttcctca ccttgcgtcc
     5701 agatctgacg ccgcatacgt cgggtactgt cattccatcc gacgaaataa atgcctttac
     5761 gcggattggc ttcgtaagta aaagtgatac tgtcccggat atcgttccat gtcaaggccg
     5821 gtttggcaaa aacggtaatg ccttgtccgg aattgacaaa ttgggctgtt ttgccgttga
     5881 tcgtcacgtc ggttacacga agattgaccc cgtcaaaata tagctgatcc aaggatgcac
     5941 gcagcggttt gaaaacgtgc gtgactttgc ctttgaccag acctttgggc ggatcaaact
     6001 gcacgtccag tttcatacgt tcgatattga tcgtatgctc gcggggcgtg atttcttttt
     6061 cgtgataata acgtgccgtg ggatattcgg tatattgtcc atacgccgcg gacatgctta
     6121 ataaaaacgt gaagaataca aatagataac gcatcagttt gacctttgtt acttggcttt
     6181 aggtatttga gttttatttt atttgaaaat aaagatatga tcttctcgta cgaccgtaaa
     6241 aactaacgcc aacggatgtg atatgcaaat gctattcgcg gcatttttgc gttatggata
     6301 tgaacgccgc ttcagatacg aatttattcc gcgctgaccg tcaatagttg atcccactgc
     6361 gttgtatatt ccggcgtacg gttttcgcat ttcatgtgcc aatctttttt ccatccttcg
     6421 ctcgcatagc gcagtgtacc gttgcccata cgcgcattga tggcatctaa tacttccata
     6481 cgtcgggagg agcgttcacg cgatatgcgg tcaaacatgt ccgtttgtac ggtttctttg
     6541 ggaataagtt ccgtaaacat cacgccgcat tttttatacc gaagaccttc tttgaaaatg
     6601 ctgcgcaatg cggatgtcgc atacgcgatg agttcgccgg tgtcatccga cggtacaggc
     6661 aacgtcacga ctttgtgata ataataatga tcgtccgtaa aacgattggt catcagatac
     6721 acagtcagcg ttttcgtagc ggcgccctct tcacgcagtt tcttcgcgca gcgatccacg
     6781 taggtcgcca tggcttcagt catctcttcc agcgatgtga ccgcccggct aaacgaacgc
     6841 gatgtggtaa tgcctttttt gcgctcacgt acctgcgcca aaggcaaaca cgatacctca
     6901 cgcagttcct gcgcgatgcg cagaccgacg atagacattg tttttttgat ccatgcggga
     6961 ttggctttcg aaaattgcaa cgcgttgtaa atgccgtgcg actgcagatg cgcggtcgag
     7021 ctgcggccga ggccccatac atcaccgact tcggtcaatt caagcgtttt ttccagatac
     7081 ggggaattca ccaagctgaa cgcccctttg agtttttttg attttttggc tttttccgct
     7141 gcgattttgg ctaatgtttt ggtacgggct ataccgacgg atacgggaat acccgtccaa
     7201 cgcgagatgg tttccttgat cttgatgccg tacgtcgtaa gatcttccgt aaaaccgctg
     7261 agatctatga acgcttcgtc tatggaatac acttccacat cgggcgtgaa tcgttgaagc
     7321 acttgcatga cacgatgcga catgtcggca tataggacgt agttggacga aaaaacatgc
     7381 gcatgatgtt tatctaaaag ccctttgact tcaaaatacg gtgcggccat cggcacaccg
     7441 agcgctttgg cttcattgga ccgcgacacg atacacccgt cgttattgga aagcaccacg
     7501 accggtttac cttccaattt gggctggaaa gcacgttcgc acgaaacgta aaaattgtta
     7561 caatcaatta aggcaaatat tgattcgtgg cgatgcatga tacttatacg accttgatga
     7621 gttcacacaa aaagctcttg gtgtttaata ataactcgaa ttttgacttg acacaaaaat
     7681 tattttctgt atattcgatg tagaggggga agggaattct ctaataattc caattccaat
     7741 tccaattcca attccaattc caattccaat tccaat
//
LOCUS       DAOQGF010000136         7551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4688_length_7551_cov_7.690635, whole genome shotgun sequence.
ACCESSION   DAOQGF010000136 DAOQGF010000000
VERSION     DAOQGF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_7551_cov_7.690635"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="PK937_13855"
     CDS             complement(<1..1886)
                     /locus_tag="PK937_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33707.1"
                     /translation="MKSRKKTSLKKRSSQKTVASRTAKPVQSKSKKKSPKAIKKSKPR
                     SSSSVILSAQKTSASVITPMIRALLDSAASGILVIDHQRHIVAVNAKFCDLFNADEPT
                     LTLWKESDLVMRFTSLVRESERFTKERDLIDNENYIVSDEIEFTHMSGRFFLRQILPI
                     PDERGKIVGRLWKFRDVTYERHIAVVYRDIEKKKFEFEEKNQELENAYRRLEEMRRDV
                     VEANQLKSQFLSNMSHELRTPLNSIMALSSILLARMDGELTEEQEKQIRIIEKSGKNL
                     LALINDILDLSKIESGKMDVFFAEYAVREFADSIRMTVLPMVRESNLEMEVDVDETLD
                     IHSTDENKLKQILLNLLSNAIKFTPHGKVRLQVRPTKFADVLEFSVIDTGIGIDRENY
                     EKIFDPFRQLDGSATRKYGGTGLGLAVTKRMVELLGGKISVESEIGKGSTFKFILPAK
                     KRGDSSKPFSAQEIEAMFTRKQDEADQLITDSAINPAKKTVLLVDDDKETLYAFRKYF
                     EEEHYNILFAQDGETALAKSAQYRPDVITLDIMMPRKDGWEVLQELKKNPDTKNIPVA
                     IVSMIDNKKLGYSLGTSDYLVKPVQRDAFMKRIQKLTEMKGLRKILIVDDDLSQAELI
                     EEIL"
     gene            complement(1911..2291)
                     /gene="gcvH"
                     /locus_tag="PK937_13860"
     CDS             complement(1911..2291)
                     /gene="gcvH"
                     /locus_tag="PK937_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNH33708.1"
                     /translation="MNFPEELLYAASHEWVRLEGDIATVGVSDYAQSELGDIIFLELP
                     EIGKKVTAGQPFGSVEAVKTVSDLYAPFSGEVIEVNGVLADKAETINSDPYGNGWIVK
                     IKVSDMSAKSSLLSANKYRESTAH"
     gene            complement(2353..4764)
                     /locus_tag="PK937_13865"
     CDS             complement(2353..4764)
                     /locus_tag="PK937_13865"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33709.1"
                     /translation="MAKAEKQAVPAVGNKAILAEVTLIDAIKQIESKLAYQPESVRFA
                     QLADAYLDMGQYDQAITILTDGLQKFPYYDTARLILAKAYYRRGEKKQAQEYIREFLQ
                     SHPAHVAAHKLLGDLALEEDDVVSAVKKYRVALRFDPINKVLIQSLVDLKDQYQKLKA
                     SRSEEEDEDGVRPVVRKPVSSAERPAPTAETPVAKAQPSFEPPAEKEELKMPTEMVED
                     LIVKEMTKKKAVPVAEVVKPEPLAEPVLEEKRDELISDSASEVPTTVKQTAAETSDVD
                     EALKASAAWIDSEDVLYFYDDEEVSFAEFKQRQELKKAGRAAIMDRAALDKKIAAAGG
                     TVKKQTVPESPKEKTEEIRAKIETPDVKEVPTAPSVKKEIPNPEEKPASQPKVVKRPP
                     ERKADDYKHEVHKDDTIHRHVDSAFGSGEDTGVFITPEQETAFFEKASAEDNEVVPYS
                     EVADQDILTAEEQEAELDELEMSYKDYLDVLTDEADLEEALFKDDEEESVVADDETDA
                     ITERLAKVQAAPDDDFIDEEDAPMSYSDYLECVESDGERDEALLDDDNEIMRFAAFAE
                     WMDASDDTIDYATYQMMEGLEEQTPKNVASTESVITEEEPAISYAAYVAGLSDEADRA
                     EARLDAVTEDVPKAAQTAAIIPEKAEEKPVPEIKAVEEPVKQPEMPQTEAFVTELKQI
                     DTPKAEAKSLVAEVPPIMTVVQETPKPVVEAVKEETAKPAAVATTEEEAEVVTEEVEI
                     TEEDINPQDATMEMVEKFAQAGQFGMSYKVCKMLKMKNPTDAKVDRKVLELKRLYLWS
                     SQLVG"
     gene            complement(4785..6278)
                     /locus_tag="PK937_13870"
     CDS             complement(4785..6278)
                     /locus_tag="PK937_13870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNH33710.1"
                     /translation="MDAGSFLFYAYLACMTVLFVFSMHNFIMIYYFLKHRDDHPQPSE
                     DWVHLPEEKLPVITIQLPIFNESTVAERLILATSQLDWPKNKLEIQVLDDSTDDTVQL
                     TRACVERLAAQGYMIRLFHRTDRKGYKAGALREALEHAHGEFVAIFDADFIPEKDFLR
                     KAMPHFSQSRIGMVQSRWGYLNEDFSMLTKTQAIGLNGHFVIQQLARNRAGFFINFNG
                     TGGIWRKSCITDAGNWHDDTLTEDLDLSYRAQLKGWKFVYLNDIVVPGELPIGIHALK
                     TQQFRWTKGSVETAKKLVPKILKAELPMWTKIQSLFHLTANFSYIFVVISCLLNSSIL
                     FLHNENGRFNVILDIMAVFVLSMLGLFIFFFYSEKMINPNWKKKMIILPVLMMGSIGL
                     SISNAKAVIEGFFDIKSSFVRTPKWGTMKRKEKTRFHGVKLDALFLIELLFLAFILVS
                     LVVGIRNYYTEKANFIGMIFFNVWILVGFSMVVFLSLKHFYDNRASA"
     gene            complement(6361..6888)
                     /locus_tag="PK937_13875"
     CDS             complement(6361..6888)
                     /locus_tag="PK937_13875"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNH33711.1"
                     /translation="MNVEPVHVTMKSYFKYLFFGILLIGLIGCVYSFTGANLGELKTV
                     AVPMFDNLTAEPGIREKVTTGLTQGILDDNNLRIADFKSADAVLSGKITQVDDIVFAF
                     EGSGNQFTTSDYKITITAIIKFENRREKKTMWEDTFSGWGRYALTGTRKRSDGMDDAI
                     KMLTQNVINKVTSDW"
     gene            complement(6885..>7551)
                     /locus_tag="PK937_13880"
     CDS             complement(6885..>7551)
                     /locus_tag="PK937_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH33712.1"
                     /translation="RRADIPVLIKRFTDEYSAQTYAKFRGFTAEAMDMMVNYRWPGNV
                     RELKNFVESLITIKRGETVTAEDITKQLENFKGIDAFENDADDTMLPIPTHMTPEQAE
                     RELLYRTLLSLKRDVDDIKHFLVAKFGNVPRPLGGSDFPHAGYAPHFDKETRDGKIVE
                     TEVYNVDEGQERDSLSLSDLERDMIIKALKKFDGKRNAAARSLGLSERTLYRKIKEYN
                     LDL"
BASE COUNT         1820 a         1899 c         1640 g         2192 t
ORIGIN      
        1 tccaaaattt cttcgatcag ttcggcctgt gacaagtcgt catccacgat caaaatttta
       61 cgcaatccct tcatctccgt cagcttttga atgcgtttca taaacgcatc acgctgtacg
      121 ggttttacca aataatccga cgtaccgagg ctgtatccga gtttcttatt gtcaatcatc
      181 gaaacgatgg ccaccggaat atttttagta tcgggatttt tcttcagttc ctgcagcact
      241 tcccaaccgt ctttgcgcgg catcatgata tccagcgtga taacgtccgg acgatattgc
      301 gccgatttgg ccaacgccgt ttcaccgtct tgcgcgaaaa gaatattgta atgttcttct
      361 tcaaaatatt tccgaaacgc atacagcgtt tctttatcat catccaccaa aagcacagtt
      421 tttttcgccg gatttatcgc actgtcagtt atgagttggt ccgcttcatc ttgcttgcgg
      481 gtaaacatgg cctcaatttc ttgcgcacta aaaggtttgc tcgagtcacc gcgttttttg
      541 gccggaagaa taaatttgaa cgttgaacct tttccgattt cgctttcgac ggaaatttta
      601 ccgccgagca gttccaccat acgtttggtt acggcaagtc ccaacccggt gccgccgtat
      661 tttcgggtgg ccgatccgtc aagttgtcga aagggatcga aaattttttc gtagttttcg
      721 cgatcaatgc cgataccggt atcaattacc gagaattcga gaacatcggc aaatttggta
      781 gggcgaacct gcaatcgcac tttgccgtgc ggagtgaact tgatcgcatt ggataaaaga
      841 ttgagcagta tttgtttgag tttattttca tcggtgctgt gaatgtccag tgtctcatca
      901 acatccactt ccatttccag gttggattca cgcaccatcg gcagaacggt catgcgaata
      961 ctgtcggcaa attcacgaac ggcgtattct gcaaaaaata catccatttt gccggactcg
     1021 attttggaaa gatccaagat atcgttgatc aaggccagca aatttttacc gcttttttcg
     1081 ataatgcgaa tttgtttttc ctgttcctcg gtcaattcac cgtccatacg cgccagcaga
     1141 atgcttgata atgccatgat ggaattaagc ggcgtgcgca attcgtggct catattggaa
     1201 agaaactggg acttgagttg gttcgcttcg acgacatcgc gtcgcatttc ctcaaggcgg
     1261 cggtatgcgt tttcgagttc ctgatttttt tcttcaaact cgaatttctt cttttcaata
     1321 tcacggtaca cgaccgcaat atgccgttca tacgtgacat cacgaaactt ccaaaggcgg
     1381 ccgacgattt ttccccgttc atcgggtatc ggtaaaatct gccggagaaa aaaacgaccc
     1441 gacatgtgtg tgaattcgat ttcatccgat acgatgtaat tttcgttgtc aatcagatca
     1501 cgttctttgg taaatcgttc cgactcgcga accagcgatg taaagcgcat cactaaatcc
     1561 gattctttcc ataacgtcaa cgtcggttcg tccgcattga ataaatcgca aaacttggca
     1621 ttcacggcta cgatatggcg ttgatgatca atcaccaata taccggatgc cgccgaatcc
     1681 aataatgcgc gaatcatcgg tgtaattacc gaagcagacg tcttttgtgc tgaaagaatt
     1741 acggaggaag aagagcgggg tttgcttttt ttaatcgctt taggtgattt ctttttggat
     1801 tttgattgca cgggctttgc cgtacgcgag gccacggtct tttgggaaga tcgttttttg
     1861 agcgacgttt tttttctaga tttcatagtg caaagccgtg tgaagtccga ttaatgcgct
     1921 gttgattcgc ggtatttatt tgccgataaa agacttgatt tagcgctcat atcggaaact
     1981 ttgattttta caatccaacc gttaccatac ggatcgctgt ttatcgtttc agctttgtcg
     2041 gccagtacgc cgtttacttc gatcacttca cccgagaatg gcgcatacag atcgcttaca
     2101 gtttttaccg cttcgacgga accaaagggc tgacctgcgg taactttttt gccaatttca
     2161 gggagctcaa gaaaaataat atcacctaat tcagattgcg cataatcaga cacaccgacg
     2221 gtagcaatat cgccttcaag gcgtacccat tcgtggcttg ctgcgtaaag tagttcttcg
     2281 ggaaaattca tgacaagact cctatataaa cattgattca gggggcaacg gaaattacca
     2341 atccaccata agctaaccga ccaactggct gctccaaagg tacaggcgct tgagttccag
     2401 cactttgcga tccaccttgg catcggtcgg gtttttcatt ttcagcattt tgcaaacttt
     2461 ataggacata ccaaactggc ccgcttgagc aaatttttca accatctcca tcgtcgcatc
     2521 ttgcgggtta atgtcttctt cggtgatttc tacttcttca gtgacaacct ccgcctcttc
     2581 ttccgttgtc gccacggcgg ccggttttgc agtttcttct tttacagctt cgactaccgg
     2641 tttaggcgtt tcctgcacga cggtcattat cggaggcact tcagcaacaa gtgatttagc
     2701 ctcggccttc ggcgtatcta tttgcttcag ttctgttaca aatgcttccg tctgaggcat
     2761 ctccggttgt ttgaccggct cttccacggc tttaatctca ggaaccggct tctcttccgc
     2821 tttttccgga atgattgcag ccgtttgtgc agctttaggt acatcctccg taaccgcatc
     2881 caaccgtgct tccgcgcgat ccgcttcatc acttaggcct gcaacgtatg cggcatagga
     2941 tatcgcgggt tcttcctcgg taataacact ttcggttgag gcaacatttt tcggcgtttg
     3001 ctcttccaaa ccttccatca tctgatacgt cgcgtaatca atcgtatcat cgctggcatc
     3061 catccattcg gcaaaggcgg caaaacgcat tatttcatta tcatcatcca gcaaggcttc
     3121 gtcgcgctct ccatcgcttt cgacgcattc gaggtaatcg ctatagctca tcggcgcgtc
     3181 ttcttcgtca ataaaatcat catccggtgc ggcctggact ttagccagac gttccgtgat
     3241 cgcgtcggtt tcatcgtcgg ctacgacgga ttcctcttca tcgtctttga aaagcgcttc
     3301 ttcaagatcg gcttcatctg tcagtacgtc cagatagtct ttgtaagaca tttccagttc
     3361 atcgagctcc gcttcttgtt cttcggctgt caatatatct tgatcggcta cttcggaata
     3421 gggtacgact tcattatctt cagccgatgc tttttcaaaa aaggccgtct cctgttccgg
     3481 cgtaataaac acacccgtat cttcaccgct gccaaatgcc gagtccacat gacgatgaat
     3541 ggtatcatcc ttatgtactt catgtttgta gtcgtccgct ttacgctcag gaggccgttt
     3601 gacgactttg ggctgcgatg cgggtttttc ttcgggattg ggaatttctt ttttaacaga
     3661 tggcgccgtc ggcacttctt tgacgtcagg tgtttcgatt ttggcgcgaa tctcttccgt
     3721 tttctctttt ggactttccg gaacggtctg ctttttgaca gtacctccgg cggcggctat
     3781 ttttttatcc agcgccgcac gatccatgat cgccgcacga ccggcctttt tcagttcctg
     3841 acgctgcttg aattcagcaa acgacacttc ctcatcatca taaaaataca aaacgtcttc
     3901 gctatcgatc cacgctgccg acgcttttaa tgcttcatcc acatcggacg tttcggccgc
     3961 ggtttgtttg actgtcgttg gtacttcgct cgccgaatcc gaaatcaatt catcacgttt
     4021 ttcctcaagc accggttctg ccaagggttc aggctttact acctccgcga ccggaaccgc
     4081 ttttttcttc gtcatttctt tgacgataag atcttcgacc atttcggtcg gcatcttcag
     4141 ttcttctttt tcagccggag gttcaaagga aggttgcgct tttgctacag gtgtttccgc
     4201 tgtcggtgca gggcgttccg cggatgaaac aggtttccga accacgggac gcacgccgtc
     4261 ctcgtcttct tcttcagagc gcgaagcctt cagtttttga tattgatctt taaggtcaac
     4321 aagcgattgg atcagtactt tattaatcgg atcgaagcgc aacgccacgc ggtatttttt
     4381 taccgcagaa acgacatcat cttcttcaag tgccaaatca ccgagtaatt tatgcgccgc
     4441 tacgtgtgcc ggatgcgatt ggagaaattc acgaatatac tcctgcgcct gctttttttc
     4501 accgcgccgg tagtacgcct tggccaaaat caggcgggcc gtatcatagt atggaaattt
     4561 ttgtagtccg tcggtaagaa tagttatcgc ctgatcgtac tgccccatgt caaggtacgc
     4621 atccgcaagt tgtgcgaagc gcacactctc cggttgatac gccagtttgc tctcaatctg
     4681 cttgatcgca tcaatgagcg tcacttcagc gagaatcgct ttattgccga cggcggggac
     4741 cgcctgtttt tctgctttgg ccataggaaa aattgatatt cgcgttatgc cgaggctcgg
     4801 ttgtcgtaaa aatgcttcaa ggacaggaat accaccatcg aaaacccgac aagaatccaa
     4861 acgttaaaaa agatcatgcc gatgaagttg gctttttcgg tatagtagtt gcgaataccg
     4921 actactaaag ataccaaaat aaaagcaaga aacaacaatt ctatcaaaaa aagtgcatcc
     4981 aatttcacgc catgaaaacg tgttttttct ttgcgcttca tcgtacccca tttgggcgta
     5041 cgtacgaagg acgacttgat atcaaaaaaa ccttcgatta cggcttttgc attactgatc
     5101 gaaagcccga tacttcccat cataagcacc ggcaaaataa tcatcttttt cttccagttg
     5161 gggttgatca ttttttccga gtagaagaaa aatataaaca gacccaacat actgagtacg
     5221 aataccgcca taatgtcgag aataacatta aagcgtccgt tttcgttgtg taaaaacaaa
     5281 atggaagaat tgagcaaaca cgaaatcacg acaaaaatgt acgaaaaatt ggccgtgagg
     5341 tgaaaaagcg attggatttt ggtccacatc ggcagttcgg ctttcagaat tttcggtact
     5401 aattttttcg ccgtttccac cgaacctttg gtccagcgga attgttgcgt tttcaatgca
     5461 tggataccga tcggcaattc gccgggcacc acaatatcat tcaaataaac gaacttccat
     5521 cctttgagct gcgctctgta actcaaatcc aaatcttcgg tcagtgtatc gtcgtgccaa
     5581 ttaccggcat cggtaataca ggatttgcgc cagataccgc cggtaccatt gaaattaata
     5641 aaaaagcctg cacggttacg cgccaactgt tgaatcacaa aatgtccgtt gagcccgatc
     5701 gcctgtgttt tagtgagcat cgaaaaatct tcgtttaaat agccccagcg actttgcacc
     5761 ataccgatcc gggattggga aaaatgcggc atcgctttac gcaggaaatc tttttccgga
     5821 ataaaatccg catcaaagat cgcgacaaat tcgccgtgcg catgctccag cgcttcgcgc
     5881 aaagcgccgg ctttgtaacc ttttcgatcc gtccgatgaa acagacgaat catgtaacct
     5941 tgtgcggcca agcgttcgac gcatgcccgc gtcaattgga cagtatcatc cgtcgaatca
     6001 tccagcacct gaatttccag tttgtttttc ggccaatcca actggctggt cgccaaaatc
     6061 agccgttcgg ctaccgtact ttcattgaaa atcggtaact gaatcgtgat caccggaagt
     6121 ttttcttccg gtaagtgtac ccagtcttct gacggttgcg gatgatcatc acgatgcttc
     6181 aaaaaataat agatcatgat gaaattgtgc atgctgaaaa caaacagcac cgtcatacac
     6241 gcgaggtacg cgtagaataa gaaagaacct gcatccatgg taaagaacat ctcgagttta
     6301 gggtttaggg tattagggtt ttttaaaggg taaaaaaatt agggttttta ttactcatag
     6361 ttaccaatcc gacgtgacct tattgatcac gttctgcgtg agcatcttga ttgcatcatc
     6421 cattccgtca ctgcgtttgc gggtgccggt gagtgcgtaa cgcccccatc cggaaaatgt
     6481 gtcttcccac attgtttttt tctcgcggcg attttcaaat ttaatgatcg ccgtaatagt
     6541 gatcttgtaa tccgatgtgg taaactgatt gccgctgcct tcgaaggcaa acacgatatc
     6601 atcaacttgg gtgatctttc cgctcagcac ggcatcggca gatttgaaat cggctatgcg
     6661 caggttattg tcgtccaaga taccctgcgt cagaccggtc gtgacttttt cacgaatacc
     6721 gggttcggcc gtcaaattat cgaacatcgg aacggcgacg gttttcagtt caccgaggtt
     6781 ggccccggta aacgaataga cgcatccgat aagaccgatc agcagtatgc caaagaacaa
     6841 atatttgaaa taacttttca tcgttacatg taccggctcg acgttcacag atcgagattg
     6901 tactccttga tcttgcgata caacgtgcgc tcagacagtc ccaaggaacg cgcggcggca
     6961 tttcgtttgc cgtcaaattt tttgagcgcc ttgatgatca tatcccgttc cagatcgctc
     7021 agcgaaagac tgtcgcgctc ttgcccttcg tccacgttat acacttccgt ttcaacgatc
     7081 ttaccgtcgc gggtttcttt gtcaaagtgc ggtgcatatc cggcgtgcgg aaaatcgctg
     7141 ccgcccaaag gccgcggtac gttgccaaac ttagcaacca aaaaatgttt gatatcatcc
     7201 acatcccgtt tcagcgacag aagcgttctg tacaacagtt cccgctccgc ttgctccggt
     7261 gtcatatgtg tgggaatcgg caacatggta tcgtcggcgt cattttcaaa cgcatcaata
     7321 cctttaaagt tttccaactg cttggtaata tcttccgccg ttaccgtttc accgcgcttg
     7381 atcgtaatca ggctttcaac aaaattcttc aattcgcgta cgttgccggg ccagcggtag
     7441 ttgaccatca tatccatcgc ttccgcggta aaaccgcgaa acttggcata cgtctgcgcg
     7501 ctatactcat cggtaaaccg cttgatcaat accggtatat ccgcccgccg c
//
LOCUS       DAOQGF010000137         7483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4770_length_7483_cov_6.775714, whole genome shotgun sequence.
ACCESSION   DAOQGF010000137 DAOQGF010000000
VERSION     DAOQGF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4770_length_7483_cov_6.775714"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PK937_13885"
     CDS             <1..736
                     /locus_tag="PK937_13885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNH33713.1"
                     /translation="SDFVCELDNTIEVTFSISGNVRPLPENVAEEKCGPADQRLNGFQ
                     IINSPIFRKYFSDELLSEQENLSVRHVAIMFTDLKGSTQMYQQLGDARAFKIVRQHFD
                     VLEQAIHAHGGVIVKTIGDAVMASFAKTSEAVGAAIDAQKQFRTFNESMQLQDGVTIR
                     IGIHVGNSLMVTLNNRLDYFGTMVNIAARVEALGDGNEILITRNVFDDLGVKNELLSR
                     AQSIQPFTARLKGIRDEQKVMKVVYN"
     gene            868..2043
                     /locus_tag="PK937_13890"
     CDS             868..2043
                     /locus_tag="PK937_13890"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNH33714.1"
                     /translation="MSKPSVKPQNTFFSSGPCAKRPGWSADQLPSRTVGRSHRAKVSK
                     TVIEEVIAKSKQILGVPDDYRLGIVPGSDTGAIEMALWNFFGHPSIESRGIDMLAWEN
                     FGYEWVYDVEKQLKIANVRPMVVPYGQIPDLTQVDFHHDVVFTWNGTTSGVCVPDATW
                     IPDDREGLTICDATSAVFAMPIDWKKIDVLTYSWQKSMGGEGAHGVLILSPRAIQRLE
                     SFPPIIAMPKVFTILKKGKLNEEIFTGATINTPSMLCIEDALDSLNWMIREGGLTAMI
                     ARSQQSLSEIKTWIASSPRYAFLAENPKTISNTSVCLKLKSDMYLSLSPDAQVKFAKD
                     VAALLEKEGAAYDIGSYRDAPPGLRIWCGTTINPADVKSLLPWLDWAHDEIARTLKF"
     gene            2057..3646
                     /gene="serA"
                     /locus_tag="PK937_13895"
     CDS             2057..3646
                     /gene="serA"
                     /locus_tag="PK937_13895"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNH33715.1"
                     /translation="MLKILISDKLSPAATEIFQRHGITADVRHGLTKEQLIEIIADYD
                     GLAVRSETKVTRDVIERATRLKVIGRAGIGVDNVDVPAATARGIVVMNTPFGNSVTTA
                     EHAVAMMMALARRIPQASTSTHAGKWEKSKFMGVELTGKTLAVIGCGNIGSIVADRAR
                     GLRMRVLAYDPFLTRERAESLGIEKTELDDAIAQADFITLHTPLTDATRHLFDAARLA
                     RCKKGARIINCARGGLIHEPALKSAIESGHIAGAALDVFEVEPAKENILFGMEHVVLT
                     PHLGASTNEAQENVALQIAEQMSDFLNTGAITNAVNIPSISAAEATALKPYFKLARQI
                     GTLAGQLQQSPIQSIAFEYEGHVAALNTKPLSLLLAQGVMAVFDQNVNMVNALYIAKE
                     RGMTVSETKIESEGNYKTLIRMRIVTEKESFSCSGTLFDGDKPRIVEVNGITLEAVLS
                     ASMLFSTNDDKPGFIGNLGKILGDADINIAHFHLGRNQGKTEAMALVTVDSPVHSDVI
                     STIKKLPSVRRVVALDFSGVV"
     gene            3834..5399
                     /locus_tag="PK937_13900"
     CDS             3834..5399
                     /locus_tag="PK937_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33716.1"
                     /translation="MMPAPTGTPLRLSQLDSLIVTSDTMPFLDKQKLCAILGHFPADA
                     WHQKPITLQMYPDFESKWSATKNTIEYSYDRSTRTIHLVDMKLARLRLNEIIAAYIAE
                     LCYGEKYPRLTEGLAMLYCGTYYGMPLPWWDKKSALLGLTNKERLITTPENFENPFLY
                     TILAVQFWQKHHDQVHLVFANPDSFIAKFEPALQVAPPAHVFPPDSIPYFKGVCFAHT
                     NGVGSGYMSKQSRTSMRRLKDIGVDAISLTPFGYAKSTTEPRVHFVLDRHWDETLGGL
                     FKAADDAHDLGIHVMMKPHIWVRGAWCGEIDIRDSTDRIAWENSYAQYATYQGLIAEL
                     AGMESMCIGLELPRMTRDTAMWKRIIHHVRIAYSGIITYGGNWFDEYEHIGFWDDLDF
                     ISIHAYFPLAHTVATPPDSSILMARQVAERLAVLSSQWKKPVAFTEVGFPSGTRGLIS
                     PHEEFDDAPADFDKQAFAYRTILETYQNKPWFKGYFWWKWESANSELKRFDKMLQFRG
                     KPAERVLQEFYSR"
     assembly_gap    5474..5573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5574..5909)
                     /locus_tag="PK937_13905"
     CDS             complement(<5574..5909)
                     /locus_tag="PK937_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH33717.1"
                     /translation="MGNTIRLHRVLKATPEKIYRAFTDADAMARWLPPHGFTCKVHHM
                     DAKIGGTFRMSFTNFTTMKSHAFGGEFLELTPYERIRYTDKFDDPNLPGEMMVTVVLK
                     KVLCGTELNI"
     gene            complement(6085..6795)
                     /locus_tag="PK937_13910"
     CDS             complement(6085..6795)
                     /locus_tag="PK937_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNH33718.1"
                     /translation="MRILVFGANGATGKLVVSQLVNKNISVRIVIRPTALIPSEISEN
                     KNVEIVRGNIHEFSMDQVSALIDDCDAVVSCLGHNLTFKGIFGKPHQLVFSIVKKITD
                     VWSASQNKKKFVLMSTTGYTNKAHGEKETFGENVVFSLLKLLLPPHTDNVNAGDYLFG
                     RVKDSKQLEWVAVRPDSLIDEMRVSEYEIVDTRKRSPIFDAGKTSRVNVAHFMVEPLT
                     DDALWEAWKYKAPVLYNK"
     gene            complement(6998..>7483)
                     /locus_tag="PK937_13915"
     CDS             complement(6998..>7483)
                     /locus_tag="PK937_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343352.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNH33719.1"
                     /translation="AASFAVLEPVADGFRNYLKADYDVPAEELLIDKAQLLTLTAPEM
                     TALIGGMRVLNVNHGQTKHGVFTHRPETLTNDFFVNLLDMRTAWVASPGAKGVFEGRD
                     RTTGELRWTGTRVDLVFGSNSQLRALAEVYAQSDAQKKFVQDFVAAWTKVMNADRFDL
                     A"
BASE COUNT         1981 a         1805 c         1628 g         1969 t
ORIGIN      
        1 aagcgatttt gtatgtgagc ttgataacac catcgaagtg acatttagca tcagtggtaa
       61 cgtacgtccg ctgcctgaaa atgtggctga agaaaaatgc ggacccgctg atcaacggtt
      121 gaacggattc cagatcatca attcaccgat ttttcgtaag tacttttccg atgaacttct
      181 ttccgagcag gaaaatttgt ccgttcgcca tgttgcaatc atgtttaccg atctcaaagg
      241 ttcgacgcaa atgtatcagc aactcggcga tgcgcgggct tttaaaatcg ttcgacaaca
      301 ttttgacgta ctcgaacaag ccatccatgc tcacggcggt gtgatcgtca aaactatcgg
      361 cgacgccgtc atggccagtt ttgctaaaac atccgaggcc gtcggcgccg cgatcgatgc
      421 ccaaaaacaa ttccgcacgt ttaacgaaag catgcagtta caagacggcg tgacgatacg
      481 catcggtatt cacgtgggta attctttgat ggtgacgctc aataaccggc ttgattattt
      541 cggtacgatg gtcaatatcg ctgcccgtgt cgaagcgctt ggggatggca atgaaattct
      601 gatcacgcgc aatgtatttg atgatctcgg cgtaaaaaac gaattattgt cacgcgcaca
      661 gagtattcag ccgtttactg cgcggctcaa aggaattcgc gacgaacaaa aggtcatgaa
      721 agtagtgtat aactaatttt taccgttcgt tatgcgctca taataatttg catattttta
      781 ttgagagaat gttatattaa agcgtgatgc atgcgatcac gctttttgtg ttctctatat
      841 acccattaca ataaaagtac atcctttatg agcaaaccat ccgtcaaacc tcaaaacaca
      901 tttttttcat ccggtccttg cgccaaacgt ccgggttgga gcgccgatca actcccgtca
      961 cgtaccgtag gacgttctca tcgcgctaaa gtttcgaaga ccgtaatcga agaagtcatt
     1021 gcaaaatcca aacaaattct cggtgttccc gatgattacc ggctcgggat cgtacccggc
     1081 tcggataccg gtgccatcga aatggcgtta tggaattttt tcggccaccc ttccatagaa
     1141 tcgcggggca ttgatatgct ggcttgggaa aattttgggt atgaatgggt ttatgatgtc
     1201 gaaaaacaat tgaaaatagc caacgtacgc ccgatggtag ttccatacgg ccaaatcccg
     1261 gatctcacgc aggttgactt tcaccacgat gtggttttta cgtggaacgg cacaacctcc
     1321 ggtgtttgtg ttcctgatgc aacgtggata cctgacgatc gtgagggtct gacgatatgc
     1381 gatgcgactt cggccgtatt tgccatgcct atagactgga aaaaaataga tgtgctcacg
     1441 tattcgtggc aaaaatccat gggcggtgaa ggtgcccatg gtgttttgat cctatcgccg
     1501 agggccattc agcggctgga gtcttttcct ccgatcatcg ccatgcccaa agtttttacg
     1561 attttaaaaa aaggaaaact caacgaagag atcttcaccg gtgccacgat caatacccca
     1621 tccatgttgt gcatcgaaga tgccttggac tcattaaatt ggatgatccg tgaaggcgga
     1681 ttaacagcta tgatcgcgcg ttcgcagcaa agcttatcgg aaataaaaac atggattgcc
     1741 tcatcgccgc ggtatgcttt tctcgcagaa aatccgaaaa cgatttccaa tacatccgtc
     1801 tgtctaaaat tgaagtccga tatgtatctg tcgctgtctc cggatgcaca agtcaaattt
     1861 gccaaagatg ttgccgcatt gctcgaaaaa gaaggtgcgg cttacgacat cggttcctat
     1921 cgtgatgcac ctcccggtct gcgcatctgg tgcggtacga cgatcaaccc ggcggatgta
     1981 aaatcattac ttccttggct tgattgggcg catgacgaaa ttgcacgtac tttgaaattc
     2041 taaaccgaaa aatattatgc ttaaaattct tatctccgac aaactcagcc ctgccgctac
     2101 ggaaattttc caacggcacg gcattacggc ggatgttcgc cacggattga ccaaagagca
     2161 actgatcgaa ataatcgcgg attacgacgg acttgccgtg cgctctgaaa ccaaagtcac
     2221 acgcgacgtg atcgaacgcg ccacacggct caaagtcatc ggacgggccg gtatcggcgt
     2281 ggacaatgtg gatgtgccgg cggccacggc acgcggtatc gtcgtgatga atacgccgtt
     2341 tggcaattct gtcactacgg ccgaacacgc cgtggctatg atgatggcgc ttgcccggcg
     2401 cattccgcaa gccagcactt ccacccatgc cggcaagtgg gaaaaaagta aatttatggg
     2461 tgtggaattg accggaaaaa cacttgccgt aatcggttgc ggtaatatcg gcagcattgt
     2521 tgctgaccgc gcacgcggat tgcgaatgcg tgttttagcg tatgatccgt ttttaacacg
     2581 ggaacgtgcg gagtcgctcg gcattgaaaa aaccgaattg gacgacgcga tagcgcaagc
     2641 tgattttatc acactgcata caccgctcac cgacgcgaca cgccatcttt tcgatgcggc
     2701 acgattagca cgttgcaaaa aaggcgcacg aattatcaac tgcgcacgcg gcggacttat
     2761 tcacgaaccg gcattgaaat cggccatcga gtcgggacat atcgccggag ccgcactgga
     2821 cgtgtttgaa gtagagcccg ccaaagaaaa tattttattc ggcatggagc acgtcgtgct
     2881 gacgccccat ttgggcgctt caaccaacga ggctcaggaa aacgtcgcat tgcaaatcgc
     2941 ggaacaaatg tccgattttc tcaacacggg tgcgatcacc aatgcggtga atattccctc
     3001 catatccgcc gccgaagcca cggcgctcaa accctatttc aaattagcgc gccagatcgg
     3061 aacattagca ggccaactcc agcaatcgcc tattcaatca atcgcctttg aatatgaagg
     3121 acacgttgcg gcgctcaata ctaaaccgct cagtttactt ttagcgcaag gtgtcatggc
     3181 cgtatttgat caaaacgtca acatggtcaa cgcgttatat attgctaaag aacgcggcat
     3241 gaccgtaagt gaaactaaaa tcgaatccga aggaaattac aaaactctaa ttcgcatgcg
     3301 gatcgttacc gaaaaagaat cattcagttg ctcaggtaca ctctttgacg gcgacaaacc
     3361 gcgcatcgta gaggtcaacg gcatcacact ggaagccgtt ctaagcgcat ctatgctttt
     3421 cagtaccaat gatgacaaac ccggttttat cggtaatctt ggaaaaattt taggcgacgc
     3481 ggacatcaat atagcccact ttcatctcgg tcgtaatcaa ggcaagaccg aagcgatggc
     3541 actcgtaacc gtcgattcgc ccgttcattc tgatgtcatc agtacgataa aaaaattgcc
     3601 cagtgtacga cgtgtcgtgg cattagattt ttcgggcgtc gtttaaattt taatagaata
     3661 cggcttacat cgtttatcaa acccctcgcg gtttgcttcg cttgttatca attcaacgaa
     3721 tttttacaga aagttctttc atgggttttt tgttgtcccc aaagcggatt aatccattac
     3781 acaccgtaat cgctttgata ttcgggttgc tccatactca ctgcggttct tatatgatgc
     3841 ctgcaccaac tggaactccg cttcgtttgt cgcagctgga cagccttatc gtgacatccg
     3901 atacgatgcc ttttttagac aaacaaaaac tctgtgcgat attaggccat tttcctgcag
     3961 atgcatggca tcaaaaaccg atcacgcttc aaatgtatcc cgattttgaa tccaaatgga
     4021 gcgccactaa aaacacgatt gaatattcat acgatcgcag tacacgtact atccaccttg
     4081 tggatatgaa actggcccgc ttgcgattga atgaaattat tgcggcgtat atcgccgaat
     4141 tgtgttatgg ggaaaaatat ccgcgcctca ccgaggggct cgctatgctg tattgcggga
     4201 catattacgg aatgccttta ccttggtggg ataaaaaatc agcgttgctc ggcctgacaa
     4261 acaaagagcg attaataact acacctgaaa attttgaaaa tccttttttg tacacgatct
     4321 tagctgtgca gttttggcaa aaacaccacg atcaagttca tttggtcttt gcaaaccccg
     4381 actcctttat tgcaaaattt gaaccggctt tgcaagtggc gccaccggca cacgtgtttc
     4441 cgcccgacag cattccgtat ttcaaaggtg tttgtttcgc acatactaac ggtgtcggca
     4501 gcggctatat gtccaaacaa tcccggactt cgatgcggcg actgaaggat ataggtgttg
     4561 acgccatttc actcacacca tttggttatg cgaaaagcac gaccgaacct cgcgtacatt
     4621 ttgttttaga tcgtcattgg gatgagacgc ttggaggttt attcaaagcc gccgatgatg
     4681 cgcatgacct tggcatccat gtcatgatga aacctcatat ctgggtacgc ggcgcctggt
     4741 gcggcgaaat tgatattcgt gactcaaccg accggatcgc atgggaaaat tcctatgctc
     4801 aatatgcgac gtatcaaggc ctcatcgcgg agcttgccgg tatggagtcc atgtgtatag
     4861 gtcttgagct gccccggatg acacgcgata ccgccatgtg gaaacgtatc atccatcacg
     4921 tacgcattgc atacagcggt atcatcacgt acggcgggaa ttggtttgat gaatacgaac
     4981 acatcggctt ttgggatgat ctcgatttta taagcattca cgcctacttc ccgctcgcac
     5041 acaccgtcgc tacaccgccg gattcgtcca tcctgatggc acgtcaggtc gcggaacgcc
     5101 tcgccgtttt gtcatcgcag tggaaaaaac ctgtcgcgtt tacggaagtt ggtttcccga
     5161 gtggcacccg tggacttatc agtccgcacg aagaatttga tgatgcccct gcggattttg
     5221 acaagcaggc tttcgcttac cgtacgattt tagaaacata tcagaataaa ccctggttca
     5281 aaggttactt ttggtggaaa tgggaaagcg ccaactctga attaaaaaga tttgataaaa
     5341 tgctgcaatt ccgaggaaaa cctgccgaac gcgtgttaca ggaattttat tcgcgttaat
     5401 ttaacataag cgtgaaccta agtgaaacaa cggactgtcc gtattagaat tcatctctca
     5461 ccaagacgct aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatgttc
     5581 aattccgtac cgcacaaaac tttttttaaa accaccgtaa ccatcatttc gccgggaaga
     5641 ttgggatcgt caaatttatc cgtgtagcga atacgttcat acggtgtaag ctccaaaaat
     5701 tcaccgccaa acgcatgact tttcatcgtt gtgaaattag taaacgacat tctaaacgta
     5761 ccgcctattt ttgcatccat gtgatgtact ttgcacgtga atccgtgcgg cggcagccag
     5821 cgcgccatcg catcggcatc tgtaaatgcg cggtagattt tttccggagt agccttcaaa
     5881 acgcggtgta gacgaatcgt gtttcccata tcaactcctt ttgttataca agattcaaat
     5941 taaacctgta ctctttatat cactgtcatc cataaatctg atactttatc accgagcgaa
     6001 ataacatccg ccgagggtta tagcgtttga cgtacagtct ctttttttat cctaaaaatc
     6061 aaaacttcgc cgaacttttt acatttattt gttatacaat accggtgctt tgtatttcca
     6121 tgcttcccat aatgcatcgt cggtcagggg ctcgaccata aaatgcgcca cattgactcg
     6181 actcgtttta ccggcatcaa atatcggact acgttttctg gtatcaacga tctcatattc
     6241 gctgactcgc atttcatcaa tcagtgaatc cggtcgaacc gcaacccatt ctaattgttt
     6301 tgaatctttt acacgaccaa acaaataatc ccccgcattc acattatcgg tatgcggcgg
     6361 caaaagtaat tttaacaacg aaaacactac gttttctcca aacgtttctt tttcaccgtg
     6421 tgctttattt gtatatcctg tagtgctcat cagcacgaat ttctttttgt tttgcgaagc
     6481 gctccacacg tcggtgatct tttttacgat actaaaaaca agttgatgcg gtttaccaaa
     6541 tatccctttg aatgtcaaat tatgaccgag acaagacacc accgcatcac aatcgtctat
     6601 gagcgcactg acctgatcca tagaaaactc atgaatattc cctcgtacga tttctacgtt
     6661 tttattctca gaaatctcac tcggtattag cgccgtcggt cgtataacaa ttctcacgga
     6721 tatattcttg ttaaccaatt gtgatactac caatttccct gtagccccgt tggctccgaa
     6781 tactaaaatt ctcataaaac cacctctctg ctatttataa atccgagggc aatataagaa
     6841 atagttttat tgataggtta acttctttgt gcgatgattt tcgcagatac ccaagatgtt
     6901 actgattagc tgcatggcat ttttactgaa taaaaaaacc ggcaagctac atataacact
     6961 tgccggttat ctcaattaga ttatcgtctg gccaatatca tgcaagatca aagcgatcgg
     7021 cgttcatcac tttagtccaa gcggccacaa aatcctgtac gaattttttc tgcgcatcac
     7081 tttgtgcata tacttccgcc aatgcgcgta attgggaatt ggaaccaaaa accaaatcta
     7141 cgcgagtacc ggtccaacgc aattcgccgg tcgtacgatc gcgtccttca aatacgcctt
     7201 tagcgcccgg agaggccacc cacgccgtac gcatatctag cagattaaca aaaaaatcat
     7261 tggtcaatgt ttccggacga tgcgtaaaca cgccgtgttt ggtttgacca tgattgacat
     7321 tcaatacacg cataccgccg atgagcgccg tcatttccgg tgcagtgagt gtcagcaatt
     7381 gcgctttatc tatcagaagt tcctcggccg gcacatcgta atcggcctta aggtaattcc
     7441 ggaatccgtc ggcaaccggt tcgaggacgg caaaagaagc ggc
//
LOCUS       DAOQGF010000138         7431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4836_length_7431_cov_7.021285, whole genome shotgun sequence.
ACCESSION   DAOQGF010000138 DAOQGF010000000
VERSION     DAOQGF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4836_length_7431_cov_7.021285"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK937_13920"
     CDS             complement(<1..747)
                     /locus_tag="PK937_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNH33720.1"
                     /translation="MKAILAEREKNTSRLVISEVDEPMVHPNDVLIAIKATALNRADL
                     LQRRGLYPPPAGVPDILGLECAGLILSVGSNVTQWSPGQRVCALLPGCGYAEKAVVHE
                     NMIIPIPEKLSFAQAAAIPEAWFTAYLNLVTIAQLKAGEAILIHSGASGVGTAAIQLA
                     KNLNAKVIASVGNSEKMRFCYELGADFVFDYHAKDFWNLIKTRCGGVNVVLDTVGGEN
                     MSHHVEVANNKARIVVIGLLGGTSSNLPTGT"
     gene            937..1698
                     /locus_tag="PK937_13925"
     CDS             937..1698
                     /locus_tag="PK937_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH33721.1"
                     /translation="MNVGTQHHVSDKKLSASSKSGVVWPALTEREIIRIAVEDTTRYE
                     PAMSEIWERYKKRLYLYIKYKLNINKGFETDKERYIIEDILQNTFCDVLENLNEYNPQ
                     YEVSTWIYNICNKHIVRYIREVQKYQGRTIAIEDESVEVIRLSSDSVKPDQEFEVKEF
                     ERIITFFVKSLQHKADQEVFLLYLQNLHNHNISDLLGKTQDAVRARLNRVIKRFKKFM
                     TKQYPEYLNSTMLSQIKNLSFGSANTENDFSPERG"
     gene            1713..2786
                     /locus_tag="PK937_13930"
     CDS             1713..2786
                     /locus_tag="PK937_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33722.1"
                     /translation="MKQLQNKIEVTADELFEQFVRELENYSTNGVVRSFETDRFWPLA
                     QHYIAQCAPMQERMDLEERIRLLLASKYQYTLQESRLKLSENLEQSIKEKIRALVRSN
                     IENAGKFAALAFNWTVIKPLQFAKDILQPVATNAMLTYAVAIQPSFTPAMDAIQLESK
                     KQISHIEVADEIKTDFLRVYKLPSVKTYSNEDLNAMNAGANTLADEKAPIIQKNVNKT
                     YHITPVQSSIKETSKLTIDVERARLIPESIPSVASSPVSYDRTRRELRSIGEVQQTIG
                     LHENKIFSCYQSYRKTSNRKNGRVAVKFLVSSKGVIKDVQVTANSFNLQMADRIVVQL
                     KAIKFSEVESRLGDQTIYHTFYF"
     gene            2839..3117
                     /locus_tag="PK937_13935"
     CDS             2839..3117
                     /locus_tag="PK937_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33723.1"
                     /translation="MLLKYGLIYMPASINIIAKRIGISETDRNLEVLKKELDKLVSKK
                     KAYRSGNRYAIDAEGARAISEYQDPKKLQEKPNPENFKKIFKQYSVAR"
     gene            complement(3157..3519)
                     /locus_tag="PK937_13940"
     CDS             complement(3157..3519)
                     /locus_tag="PK937_13940"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNH33724.1"
                     /translation="MVIVDSKTGKTTFSVHLSEAESVNLLGNFNGWNEDKHPLKKDKS
                     GNWSITLKLTTGEYEFLYRVNKKAWMVDDKAPRKTNPFGTENCLVSVVIDMAKRANTE
                     KKSAVRTNKAKSSKTKKA"
     gene            3742..4308
                     /gene="efp"
                     /locus_tag="PK937_13945"
     CDS             3742..4308
                     /gene="efp"
                     /locus_tag="PK937_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNH33725.1"
                     /translation="MAGVADFRTGMTILMDGNLFVVTEFQHRNPGNWRAFVVAKLKNV
                     RTGRVIEKTFRSHDKLEEVRAESKNMAYLYSDGDMMYFMDNESFEQVGIPRESVGEAA
                     NYLKEGIAPAILFYEGNAVSIEMPITVELKITSTVPGVKGDTVTGGTKPATLESGAVV
                     NVPLFINEGDTVKVDTRSGDYLERVNTK"
     gene            4338..4865
                     /gene="accB"
                     /locus_tag="PK937_13950"
     CDS             4338..4865
                     /gene="accB"
                     /locus_tag="PK937_13950"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNH33726.1"
                     /translation="MDLKDLKNLIKMVDESNVNEVEIEQDNAKTKGRLKIRISKSGTT
                     HAMAVVQHSAPMAMPHHVPTQHLPPPAVQFESQETIEKSKSERPAGAVEQGNFIEVKS
                     PIVGTFYRSPAPDADVYVQVGSSVKPGQTLCIIEAMKIMNEIESESSGKIAKILVENG
                     QPVEYNQTLFLIEKA"
     gene            4887..6395
                     /locus_tag="PK937_13955"
     CDS             4887..6395
                     /locus_tag="PK937_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH33727.1"
                     /translation="MSFRKKIYITGFLLITTWLFAGFYLLMHYQSTQELTDTLIPYEI
                     VKLKSIENLRRHADSFERFWAPTEETPIHPIVLNQELIQFDEEILSLIKLHWESKALW
                     EAHGDHVGSSSLVMYVTGLIWYDTLSVDSERILLSHLKELAAQLRNSAPTRAGTTIAL
                     TEKKILGTIHEIQVSIHALDETANKILEQKISHISHRGLFAKSIIFTVFTLAIILITF
                     LIIDIHRILQSIGGIKTITSAFAEGKLEERLPVEGFEEIRTIAISFNAMGERMQELDK
                     LKSDFFNKLVHDFKSPLDNIKQSADVLISGITGETSDGKQKKFLEIIKKSSIQLRTMI
                     QEQLDESKLIAGQSSLVYEQTDLRQLIRERMELQRPTATARQLNFAVKFTDATYVIPC
                     DRAKLSRVMDNLLSNAIKYAFPETTISVEIEDRAHEIELRVRDHGPGIPATMLTQIFQ
                     KYVRIEHNRSTSGTGLGLYTAKYIVELHKGRIWASNYNDGAVLHVLLPKSPG"
     gene            6478..6551
                     /locus_tag="PK937_13960"
     tRNA            6478..6551
                     /locus_tag="PK937_13960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6512..6514,aa:Arg,seq:tct)
     gene            complement(6719..>7431)
                     /locus_tag="PK937_13965"
     CDS             complement(6719..>7431)
                     /locus_tag="PK937_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNH33728.1"
                     /translation="KLVYRKYISYAVGISIAVHLMAVGLYMAITLWDWSDGKQEAPMV
                     FKLENLDFLLNDSKPKSSGSRASMRAKVSSDKDLKKMANVSEIVKDEKEADKKITDSM
                     VVDTTAQFFAENGTGNGLGRGIGMGVGDEGGDGASSGKEKPVVHTNPRVRVLVQPNVE
                     KVKNKINGSVRLRLHIDNQGNVVEAVVVNNTTKDKELQEIARKTANRMKFYPATNDGV
                     PAEDWFDHEIVFNFDGKK"
BASE COUNT         2297 a         1640 c         1472 g         2022 t
ORIGIN      
        1 tgtacccgtc ggtaaattgg aagacgtacc gcctaatagc ccgattacga cgatgcgcgc
       61 cttgttgttg gcgacctcta cgtgatgtga catattttcc ccgccaacgg tatccaatac
      121 gacattcacg ccgccacaac gcgtctttat aagattccaa aaatctttgg cgtgataatc
      181 aaatacaaaa tcggcgccta attcataaca aaagcgcatt ttctcggagt tgccgacaga
      241 agctataacc tttgcattta aatttttagc taattgtata gcagcagttc ctacaccgct
      301 tgctccggaa tgaatgagga tggcttctcc ggctttaagt tgggcgatcg tgacaagatt
      361 gagatacgcg gtaaaccagg cttccggtat cgctgcagcc tgagcaaaac tcaacttttc
      421 cggtatcggt atgatcatgt tttcatgaac aactgctttt tctgcatatc cgcagcccgg
      481 caaaagagca cacacgcgtt gccccgggct ccactgcgtc acatttgagc ccactgaaag
      541 aataaggcca gcacattcca atcccaaaat atcgggaaca cctgccggcg gcggataaag
      601 cccacgcctt tgaagtagat cagcacgatt gagagctgtt gctttgattg cgatcaacac
      661 atcatttgga tgaaccatgg gttcatcaac ttcactgata accaatcgtg atgtgttttt
      721 ttcacgttct gcaagtatgg ctttcataaa caattaacaa cagaaaagta aagatatttt
      781 ccaaatcaaa aaactaataa atatccaatg aaattttgca tgttgtgata tacgtcacag
      841 cacgagcgaa gtatttataa tagcttgaaa accgaagtgt cactatacca aatctaagac
      901 atttttaatt aatttcaact aaaacaaaaa cttaaaatga acgtcggcac gcaacatcat
      961 gtctccgata agaagctaag tgcttcaagt aagagcggtg tggtgtggcc tgcactgact
     1021 gaacgtgaaa ttatacgtat tgctgttgaa gatacaaccc gttacgaacc cgccatgtca
     1081 gaaatatggg aacgctacaa aaaacgtttg tatctttata taaaatataa actcaatatt
     1141 aataaaggtt ttgaaactga taaagaacgt tacattatcg aagatattct tcagaatacc
     1201 ttttgtgatg ttctagaaaa ccttaatgaa tataatcctc aatatgaagt ttcaacatgg
     1261 atttataata tttgtaacaa gcatattgta cgttatatac gcgaagttca gaaatatcaa
     1321 ggacgtacta tagctatcga agacgaatct gtcgaggtta tccgattatc ctccgactct
     1381 gtgaagcctg accaagaatt cgaagtaaaa gaatttgaac gaatcataac cttttttgta
     1441 aaatcattac agcacaaagc ggatcaggaa gtgttccttt tgtatttaca gaatttacat
     1501 aatcataaca tttccgatct tttgggtaaa acgcaagacg cggtacgagc ccggctcaat
     1561 cgcgtgatca aacggttcaa aaaatttatg actaaacagt atcctgaata tctaaattca
     1621 acgatgctgt cgcaaatcaa aaacctttcg tttggttcgg ccaatactga aaatgatttt
     1681 tcacctgaaa gaggataggt ggtaagcgga ttatgaagca acttcaaaac aaaatcgaag
     1741 ttacggctga cgaactgttt gaacagtttg ttcgcgaact ggaaaattac agcaccaatg
     1801 gcgttgttcg ttcttttgaa acggaccgtt tttggccgct tgctcagcac tacattgcgc
     1861 aatgcgcacc catgcaagag cgtatggatt tagaggaacg aattcgcctt ctcttagcat
     1921 cgaaatatca gtacactttg caggaatccc gattaaaact cagtgaaaat ttagagcaaa
     1981 gtatcaaaga aaaaatcagg gccttagtca gatctaacat agaaaatgcc ggtaaatttg
     2041 ctgcgttagc tttcaattgg acagtcataa agcctttgca gtttgcaaag gatattttac
     2101 agccggtcgc aaccaatgct atgcttacct atgctgtagc gattcaacca tcattcacac
     2161 cggctatgga tgcgatacaa ctagagtcta aaaagcaaat ctcgcatata gaagtcgctg
     2221 acgaaattaa gactgatttt ttacgagttt ataaactacc aagcgtaaaa acatactcta
     2281 atgaagatct gaatgccatg aacgccggag ccaatacact ggcagacgaa aaagctccga
     2341 taattcagaa aaatgtaaat aagacgtatc acataacacc tgtacaatcg tctattaaag
     2401 agacttccaa gttgaccatt gatgtggagc gtgctcggtt gattccggaa agcatacctt
     2461 cggtggcttc ttcccccgtt tcttacgacc gtacacgacg tgaattgcga tctatcggtg
     2521 aagttcaaca aactatcggc ctgcatgaaa ataaaatttt tagttgttat cagtcgtatc
     2581 gcaaaacttc aaatagaaaa aatggacgtg tcgccgttaa gtttttagtt tcgagtaaag
     2641 gggttatcaa ggatgtacag gtaactgcca atagctttaa cctgcaaatg gcggaccgta
     2701 ttgtggtaca actcaaagct atcaagttta gcgaagtgga atcacgcctt ggcgaccaga
     2761 ctatatacca tacgttctat ttctgatcga attatttgag aaaataagta gagaggggtt
     2821 acttttatgc tggagtacat gctactcaag tacgggctta tttatatgcc ggcttcgatc
     2881 aatattatcg caaagcgtat cgggatatcc gaaaccgatc gcaatctcga agttctcaaa
     2941 aaagaattgg ataaactggt ttcaaagaaa aaggcttatc gctccggcaa ccgctatgcg
     3001 atagatgccg aaggtgcacg cgccatttca gagtaccagg atccaaaaaa actgcaggaa
     3061 aaacctaatc cggaaaactt taaaaaaatt tttaagcagt attctgtcgc acgctaggaa
     3121 aaacgtgcga attttaagaa agcctgacga taaccgttag gcttttttcg ttttagagga
     3181 ttttgctttg tttgtacgca ccgcagattt cttttcggta tttgctcgtt ttgccatatc
     3241 aattactaca gaaaccaagc agttttccgt accaaaagga ttggttttac gcggcgcttt
     3301 atcatctacc atccaggctt ttttattaac acgataaaga aattcgtact cgcctgtcgt
     3361 caattttaat gttatagacc agttacccga tttatctttt ttgagaggat gtttatcttc
     3421 attccaaccg ttaaaatttc ctaaaagatt gaccgattcc gcctcgctta aatgaacaga
     3481 aaaagtcgtc ttccccgttt tcgagtctac tattaccata agttgcttcc tctcatttta
     3541 gataaataac ttaatgaata tacctacaaa tcataaaaac acaaggaaaa gcagaaaaaa
     3601 ttacaaaatt aatgcaattt cgcagcattt acccggcaaa aatatacgtt tttataaaat
     3661 tcttgacttt ggatatgcca ttctctactt tgagcggtct aaaaaataca atcgtttact
     3721 ttctttagga ttcagattta catggcaggt gtcgccgatt ttcgaacagg tatgaccatt
     3781 ttgatggatg gtaatctttt tgttgttact gagtttcaac atcgcaatcc gggcaattgg
     3841 cgcgcttttg tcgtcgctaa attaaaaaat gttcgcaccg ggcgagtgat cgaaaagaca
     3901 tttcgttcgc acgacaaatt agaagaagta cgcgccgaaa gcaaaaacat ggcctacctc
     3961 tattccgatg gcgatatgat gtactttatg gataacgaat catttgaaca agtgggtata
     4021 ccccgtgaat ccgtcggtga ggcggccaac tacctgaaag aaggtattgc accggctatc
     4081 cttttttatg aaggcaatgc cgttagtatc gaaatgccta tcaccgttga attaaaaatc
     4141 acaagtacgg ttcccggagt taagggcgat accgtgaccg gaggcaccaa accggcaacc
     4201 ttggagtccg gtgctgtagt caatgtacct ctttttatca acgaaggtga tacggtgaaa
     4261 gttgacaccc gctccggtga ttacctcgaa cgcgtcaata cgaaataaat cttctgcaca
     4321 aacggagaga gaaacgcatg gatctcaaag acttgaaaaa cctcatcaaa atggtggatg
     4381 agagtaatgt caatgaagtc gaaatagaac aagacaacgc caaaaccaaa ggacgcctga
     4441 agatacggat aagcaaatcc ggtacgacac atgccatggc cgtcgttcaa cattcggctc
     4501 cgatggctat gccacaccat gtaccgacac aacatcttcc cccgccggct gtccaatttg
     4561 aaagccaaga gacgatagaa aaatctaaat ccgaaagacc ggccggagcc gtagaacaag
     4621 gtaattttat agaggtcaag tcgcctattg tcggtacttt ttaccgttcg cctgcacccg
     4681 acgccgatgt ttatgtgcaa gtgggctctt cggtcaaacc cggacagacg ctttgtatta
     4741 tcgaagcgat gaaaatcatg aacgaaatcg aatccgaatc atccggtaaa atcgccaaga
     4801 tcctcgttga aaacggtcaa ccggtggagt acaatcagac gcttttcttg atcgaaaaag
     4861 cttaactccc ggcgagaata aacgccgtgt catttcgcaa aaaaatatac ataaccggtt
     4921 ttttactgat aacaacctgg ctttttgccg ggttttatct tttaatgcac taccagagta
     4981 cacaggaact taccgacacg ctgatcccct atgaaattgt caaacttaaa tccatcgaaa
     5041 atcttcgccg acacgctgat tcattcgaac gattttgggc ccctaccgaa gaaacgccga
     5101 tccatccgat cgtccttaat caggaattaa tccaatttga tgaagagata ctgtcgttaa
     5161 taaagctaca ttgggaaagc aaggcgcttt gggaggcgca tggggatcac gtcggctcat
     5221 catctcttgt catgtacgtt acgggcctta tttggtatga cacgctttcc gtggactcgg
     5281 aacggatatt gctgagccat ctcaaagaac tcgcagctca gcttcgtaac tccgcgccaa
     5341 cccgtgcagg cactacgatt gctttgacag aaaagaaaat attaggcact atacatgaaa
     5401 tccaagttag catccatgcg ctggatgaaa ctgccaataa aattctggaa cagaaaatct
     5461 cacatatctc gcatcgcggt ctttttgcca aatctatcat atttacagtt tttacacttg
     5521 ctatcatttt gattacattt ctcatcatcg acatacatcg tattttacaa agtataggcg
     5581 gtataaaaac aatcacatcc gcatttgccg aaggcaaact cgaagaacgc ctgcccgtag
     5641 aaggttttga ggaaatacga accatcgcca tatcatttaa cgccatgggc gaacgcatgc
     5701 aggaattgga caagctcaaa tccgattttt ttaacaaact cgtacatgat ttcaaaagcc
     5761 cgctggataa tatcaaacaa tcggccgatg tgctgatcag tggaataacc ggcgaaactt
     5821 cggacggtaa acaaaagaaa ttcttagaaa ttattaaaaa aagttccatt caactccgta
     5881 cgatgataca agaacagttg gatgaatcca aacttatcgc ggggcaaagc tcgcttgtgt
     5941 atgaacaaac cgaccttcgt cagttgatcc gcgagaggat ggaattgcaa cgcccgaccg
     6001 ccacagcgcg ccaactgaat tttgccgtaa aattcaccga tgccacctac gtcatccctt
     6061 gtgaccgtgc aaagctatca cgtgtaatgg acaatctgct cagcaatgcg atcaaatacg
     6121 cgttccccga gacgaccata tcagtagaaa tagaagatcg tgcacatgaa attgagcttc
     6181 gagtccggga tcacggtccg ggcattccag caacgatgct aactcaaatt ttccagaaat
     6241 acgtacgtat agagcataat cgaagtacat ccggcacagg cctcggttta tacaccgcta
     6301 aatatattgt agaattacac aaggggcgca tctgggcatc gaattacaac gacggtgctg
     6361 tattacacgt tttactacca aaatcaccgg ggtaaacgcg gctcaaccat taaaaacaaa
     6421 atctgcttga aacaggaatc ctgacactct atattgcgcg cgtcaaacat atatgatggg
     6481 cccgtagctc agctggatag agcatctgcc ttctaagcag agggtcacag gttcgagtcc
     6541 tgtcgggcct actaataaac aaaggtttgc taaatctata gcaaaccttt cttatttaac
     6601 atcaagatat cttttgtaag ataacccatc aattcatccc gtaaatgcgc ttgccgtagg
     6661 ttactgattg aaaattttat attaaaaagt gaatatgtga ttcaagcggc cctgtttatt
     6721 attttttccc gtcgaaatta aaaacgattt cgtgatcaaa ccaatcttcg gccggaacac
     6781 cgtcattggt ggcaggataa aacttcattc gattagccgt tttgcgggca atttcctgaa
     6841 gttccttatc tttggttgta ttatttacaa cgacggcttc aaccacatta ccttgattat
     6901 ctatgtgtaa gcgcaatcgt acagagccgt taattttatt ttttaccttt tcaacattgg
     6961 gttgcaccaa aacccgaact cttggattgg tatgaacaac gggtttctct ttaccgctgg
     7021 aagcgccgtc gccaccttca tcccccacac ccatgccgat tccccgtcct agaccgttac
     7081 cggttccatt ttcagcaaaa aattgtgcgg tcgtatccac aaccatgcta tccgttattt
     7141 ttttatcagc ctctttttcg tctttaacga tttctgatac attggccatc ttcttaagat
     7201 ctttatccga acttactttt gcacgcatcg aagcgcgtga tccagatgac tttggcttac
     7261 tatcattgag aagaaaatcc aaattttcga gtttgaaaac catcggcgct tcctgtttac
     7321 catccgacca atcccaaagt gtgatggcca tgtataatcc gacagccatg agatgtacgg
     7381 caatggatat acctacggca taggaaatat atttacgata aacgagtttt a
//
LOCUS       DAOQGF010000139         7416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4849_length_7416_cov_6.458905, whole genome shotgun sequence.
ACCESSION   DAOQGF010000139 DAOQGF010000000
VERSION     DAOQGF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_7416_cov_6.458905"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK937_13970"
     CDS             <1..419
                     /locus_tag="PK937_13970"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNH33729.1"
                     /translation="DTPPVILKNLHRILKVRAYGYWGEHVWWNVQTGRPYSDLIGKQR
                     AYEIIINAIVPYVTLYAQRLGDKKLYETVMTCMKTWVAHEHNHIIGWMEKRLPGMPDR
                     INAEYAQGLIELHKKCRVRNCSDCRIFQRMVYSDFI"
     gene            complement(412..2652)
                     /locus_tag="PK937_13975"
     CDS             complement(412..2652)
                     /locus_tag="PK937_13975"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNH33730.1"
                     /translation="MSIQYTLQPMRWFIGISLLAMVLPAMAQHGHSLKISETDDLSAA
                     DASFYLQRASELLEQREYASALRHFVFAGEIEPSLYRQFVFQYKLAKAYRGLRQMEKA
                     IQYFSHTAADSAFGDFALVSLGELYLDVDSTNQARSVARRLLRRFPKSALIPHGYYLL
                     LKIADIQKDDAVWKATMTDLRRILSDYPKLQMQWEPDLLQREARFFIRNRENEQAQTI
                     LRRLQNDYPYSQEAYEAWPQLFSFYPSKNNPPPVDDLINALQVRMMQGHHTEALNMIA
                     ALRTWYIDDFAHKEIDFMTARIYFAQGRYDLAIPRFANLWSDHASAEALFQMGRAARY
                     ADEIERSIHAYGQYLQHGKLNDAWSSYIRFEMANNRAVRFDSAAIGEVNDGYRSVYQN
                     ASQRSHYAVASRFREGLNWYIAGTYDSAIVYWEKYMRSTPSQKTRAQYWIARAYAKKN
                     DIQKAKALYESITHTKGDEYYTTLAHMRLTSHDLRAFYFTDTTDDGNKTKKIFNPVST
                     PDIGNDPRLDRVAMAIRIGEVAWGEQEAEPLVNDYITRRDRGEKFRDYLESLHAYDLA
                     VDVNVALKKKYRSYYKIYNDDVRMFYPLYYRPLVKHHATAYRLDEALIFALIKSESAF
                     RTYALSPARAIGLMQIMPFTGKALAAETGMPDFQPSSLHEPDVSIHLGSHYLRQLADM
                     YKNYIPAVLGAYNAGPHRATFWMKAFNDEEPDIMPEVVEIFETETYIKRILVDRYKYD
                     LKLHAK"
     gene            2793..5438
                     /locus_tag="PK937_13980"
     CDS             2793..5438
                     /locus_tag="PK937_13980"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNH33731.1"
                     /translation="MKRILSAIAGFCYRHSTSVLIAVGLITVVSTYYMMQIKISTNNF
                     DLLPQNSKLIKEFWEVNEDFGAQETHILLIETNDSLPAEPEIIRDYAERLDAAFMASG
                     LVKQVNYRITDDQKSFVEDFFIRNALLYLSPQDLDSVVRRLDDANIEKAILNAKNILN
                     APIPPDPLLKRVLREDPLLLSELFVPYIEKMLGPQNASLIREKESYYMSKDKRTLLMF
                     VKPDGVANDTKFCETIVGRSHEITDSLIVAMGDNGKKLTVTLGGNYVSSLANANAVKQ
                     GLISSSFIVILLILVLFFFFFGNFRSLAFIMIPILAGVTWVFCFADLMFEKVNIITAA
                     AGAMLLGLGVDYAIHIYNRFVEQETHERHNTVEKNLDITFRETGTSVFYGAVSTAFVF
                     IVLMVTEFRGLYELGFLGGVGILILFVAVVIVMPAEIKLRKRKNPRVNYLQGAFSKLL
                     GLNSRFVLKHPKYIATGALIITIFMIGVLTGAVPSKDEGLGVTFDDNIENIRSKKDVD
                     LQMIRRLEQKFGSHFKPISVVVSATSDDALIARLHKLNAKLDELTAKGMIKEYNSMLR
                     YIPSIEQQKHNLETIRNLDVESILFKIRLEMNKNGLRMNYFRLDRLRDMLQAREPITI
                     RNFEGEGFNEIMKHFYVERNGVKKVVTQVEFAGQSYEIDIVNQFVDEFNRDEQIRNEN
                     VIITGIRVVTAEFLTLVKKDFQVAVIASLSAILLLVIVHYRKFRAVLVCMVPLTFALL
                     TIMGVMRLMGIKINFVNMITLPLLIGSGVDYGIYIISRYLEDQSHDVYAAIHETGQSL
                     FLSAATTVIGFGSLIFVDNRGLASLGYMCSFGIIICSVSSVIILPALLRLWGKKIWKE
                     AREEESPNKDTMIKKVTAAHTPEKK"
     gene            5435..5989
                     /locus_tag="PK937_13985"
     CDS             5435..5989
                     /locus_tag="PK937_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNH33732.1"
                     /translation="MTRILIVMLAFVGCTPQPIYRAVKADGHGPSVKATETHKPTASV
                     SDSSATGAKKEPANTEEAMMAEIKTWIGTPYRFGMEKKSEGTDCSGFVGAVFRKVRQI
                     ELPRQAADMYAKGTPITRSELRFGDLVFFQNTYKGAVGASHVGIYVGNNQIAHAGTTV
                     GVTISSLEESYYEKHYLGCRRISQ"
     gene            complement(6075..>7416)
                     /locus_tag="PK937_13990"
     CDS             complement(6075..>7416)
                     /locus_tag="PK937_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33733.1"
                     /translation="NKTTHPVDVVLNEQQAFVSSEFTYKVAGDQSPRSILAGASPIRD
                     MSGAMQGVVMVFSDITDRIKMEQELLKSRKIESIGVLAGGIAHDFNNILSAIMGNISL
                     AKVKIAAAQKDKAQELLSRAERASERAKELTQQLLTFSKGGAPVKKTANIADLLRESA
                     QFSSAGSNVRCEFFIQDNLYPSDVDAGQISQVLNNLIINARQAMPNGGIIRILAQNHA
                     IQSHNEGMPLKPGSYLKIAIEDNGCGISEEHLQRIFDPYFTTKQTGNGLGLATSYSII
                     KNHDGAITVDSKINVGTTFTLYLPASKFAVQQPRPQEPQALLSNGRILVMDDEESIRE
                     MASNMLNHLGFDAVSAKSGEEAVAMYEDARARNKAFDAIIMDLTIPGGLGGKDAMARI
                     LSIDPAVCGIVSSGYSNDPVMADHTRYGFQGILPKPYFINDLSRVLNQLLKPKT"
BASE COUNT         1922 a         1639 c         1834 g         2021 t
ORIGIN      
        1 gcgacacgcc gccggtaatt cttaaaaact tacatcgtat tctaaaagtc cgtgcttacg
       61 gttattgggg ggaacacgtt tggtggaatg tccaaacagg ccgtccgtat tccgatctga
      121 tcggaaagca acgcgcatac gagatcatca tcaatgcgat cgttccgtat gtcacgctgt
      181 acgcgcagcg cctaggggat aaaaaactgt atgaaaccgt catgacatgt atgaaaacgt
      241 gggtggcgca cgaacataat catatcatcg gctggatgga gaaacgttta ccgggcatgc
      301 cggatcggat caatgccgaa tacgcgcaag ggctgatcga actgcataaa aaatgccgtg
      361 tgcgcaattg cagtgattgc cgcatattcc aacgcatggt gtattcggat tttatttagc
      421 gtgcagtttc agatcgtatt tatagcgatc cacgagaatg cgtttgatat acgtttccgt
      481 ttcaaaaatt tccaccacct ccggcatgat gtccggttct tcgtcgttaa acgctttcat
      541 ccaaaacgtg gcgcgatgtg ggccagcatt gtatgcgccc aatacagccg gtatataatt
      601 tttatacata tcagcaagtt gccgaagata gtgggatccg aggtgaatgc tgacgtccgg
      661 ttcatgcaaa gacgaaggtt gaaagtcggg cataccggtt tctgccgcca gcgctttgcc
      721 cgtaaacggc atgatctgca tcaaaccgat cgcgcgagcg ggggacaatg cgtacgtgcg
      781 aaaagcgctt tccgatttga tcaatgcaaa gatcagcgct tcgtcaaggc gatatgctgt
      841 cgcatgatgc ttaaccaacg gacgatagta cagcggataa aacatgcgta cgtcatcatt
      901 gtatatttta taatagctac ggtatttctt tttcagagcg acattgacat ccacggccag
      961 atcgtacgca tgcaggcttt ccaaataatc acggaatttt tcgccccgat cgcggcgggt
     1021 gatgtaatca tttaccaatg gttcggcttc ctgttcaccc cacgccactt caccgattct
     1081 aatagccatc gctacgcggt ccaatctcgg atcattaccg atatcggggg tacttacggg
     1141 gttgaatatt tttttggttt tgttaccatc atcggtcgta tcggtaaaat aaaaagcgcg
     1201 caggtcatgc gaagtcaaac gcatgtgtgc taatgtggta tagtattcat cccctttggt
     1261 gtgcgtaata ctttcgtaca acgctttcgc tttttgtatg tcattttttt tggcgtacgc
     1321 gcgggcgatc cagtattgtg cgcgtgtttt ttgagacggt gtactgcgca tatatttttc
     1381 ccaataaacg atcgcgctgt catacgtccc ggcgatatac caattgagcc cttcgcgaaa
     1441 acgcgatgcg acggcatagt gcgaacgttg ggaggcgttt tgatatacgg aacggtatcc
     1501 gtcgttgact tcaccaatcg cggcggaatc aaagcgtacc gcacggttat tggccatttc
     1561 aaaacgaata tatgacgacc atgcatcgtt gagttttccg tgctgcaggt attgaccgta
     1621 cgcgtgaatg gatcgttcga tttcatccgc ataacgtgct gcgcgaccca tctgaaaaag
     1681 cgcttccgca gacgcatgat ccgaccatag attggcaaaa cgcggtatcg ccagatcgta
     1741 ccgtccttga gcgaaataaa tgcgcgccgt cataaaatct atttctttat gtgcgaaatc
     1801 atctatatac catgtccgta aagcggcgat catattgagc gcttcggtat gatgaccttg
     1861 catcatgcgc acctgcagtg cattgatcaa gtcatctaca ggcggaggat tgtttttgga
     1921 cggataaaaa ctaaataatt gcggccaagc ctcgtaggcc tcttgcgagt aaggatagtc
     1981 gttttgtaaa cgtcgcaaaa tcgtctgcgc ctgttcgttt tcacgattgc gtataaaaaa
     2041 acgcgcttct ctttgcagga gatcgggttc ccactgcatt tgtaatttcg gataatccga
     2101 cagaatgcgc cgtagatctg tcatcgttgc tttccatacc gcgtcatctt tttgaatatc
     2161 agcaatcttc agcaggagat aatacccatg cggaataagt gcggatttgg ggaaacggcg
     2221 caacagtcgg cgtgcaaccg accgggcttg attcgtacta tccacatcca aatataactc
     2281 gccgagagat acgagcgcaa agtcgccaaa cgccgagtcg gccgcggtat gtgagaaata
     2341 ttgaatggct ttttccatct ggcgaagacc gcgatacgct ttggccaatt tgtattgaaa
     2401 aacaaattgc cggtataatg aaggctcgat ttcgccggca aaaacaaaat ggcgtaatgc
     2461 cgacgcgtat tcgcgctgct ctaaaagttc ggaggctctt tgtaggtaaa aagacgcatc
     2521 ggccgcggag agatcgtccg tctcggaaat ttttaaggaa tggccatgtt gcgccatagc
     2581 gggtaatacc atggccaaaa ggctgattcc tataaaccat cgcatgggct gtaatgtata
     2641 ttgtatgctc atataaaaaa gtagatgttc gcgagcttta tttcaatgtg aaagcggtaa
     2701 acagtccatg ttttggtgat caataaagag gtgaattaaa ttgcacaaat aacctaaata
     2761 ttctattttg cgccactatt taggagtatc gtatgaagcg tattttgtcg gccattgcag
     2821 gattttgtta tcgtcactct acgtccgttc ttatagccgt cgggcttata accgttgtct
     2881 ctacatatta catgatgcag atcaaaataa gcactaataa ttttgatctg ttgccgcaaa
     2941 attccaaact catcaaagaa ttttgggaag ttaacgaaga ttttggcgcg caggaaacac
     3001 atatcctgct tatcgaaacc aacgattctt tgcctgccga gccggaaatc atcagagatt
     3061 acgccgagcg attggatgcg gcctttatgg cttcgggatt ggtcaaacaa gtcaattacc
     3121 ggatcacgga cgatcaaaaa tcgtttgtcg aagatttttt tattcgcaat gcgctgcttt
     3181 atctttcgcc gcaggatttg gattcggtcg ttcgacgctt agatgatgcc aatatcgaaa
     3241 aagccattct taatgctaaa aatatactca acgcgccgat tccgccggat cctctgctaa
     3301 agcgcgtttt acgcgaagat ccgctgcttt tgtccgaact gttcgtgccg tatatcgaaa
     3361 aaatgttggg ccctcaaaat gcctcattga ttcgggaaaa agagagctac tacatgtcca
     3421 aggacaaacg gacgctgctc atgtttgtca aaccggacgg cgttgcgaat gacacgaagt
     3481 tttgtgaaac gatcgtagga cgcagtcacg agattaccga ttcgcttatc gtagcgatgg
     3541 gtgataacgg taaaaaactc accgtgactt tgggcggtaa ctatgtttcg tcccttgcca
     3601 acgccaatgc cgtcaaacaa ggtctgatca gcagttcgtt tattgtgata ttattaattc
     3661 ttgtgctctt cttttttttc tttggtaatt tccgttcgct ggcgtttatc atgataccga
     3721 tacttgccgg tgtgacgtgg gtattttgtt ttgccgatct gatgttcgaa aaagtcaata
     3781 tcatcacggc ggcagccggt gcgatgttat tggggcttgg ggtagattat gcgatacata
     3841 tctataatcg ctttgtcgaa caggaaacgc atgagcggca caatacggtt gaaaaaaatc
     3901 tggatatcac gtttcgcgaa accggcacca gtgtttttta cggcgctgtc agtacggcat
     3961 ttgtttttat cgtacttatg gttacggagt tccgcggatt gtatgaactg gggtttctcg
     4021 gcggtgtagg tattctgata ctttttgtcg cggttgtgat tgtcatgccg gcagagatca
     4081 agcttcgtaa acgtaaaaac ccgcgtgtga attaccttca gggcgcattc agtaaattgc
     4141 ttggattaaa ttcaaggttt gttctcaaac atccgaaata tatcgctacg ggcgcattga
     4201 tcataaccat atttatgatc ggcgttttga caggggccgt acccagcaaa gatgaaggcc
     4261 ttggcgtcac atttgacgac aacatcgaaa atatccgcag taaaaaagat gttgatcttc
     4321 agatgatccg gcgcttggaa caaaaattcg gctcgcactt taagccgatc agcgttgtcg
     4381 tttcggcgac ttcggatgat gctctgatag cccgcttgca taaactcaac gccaaattgg
     4441 acgaattgac ggccaaaggc atgatcaaag aatataattc catgctgcgt tatattcctt
     4501 ccatcgagca gcagaaacac aaccttgaga ccattcgtaa tctggacgtc gagagcattc
     4561 tctttaaaat ccggttggag atgaataaaa acgggcttcg gatgaattat tttcgtcttg
     4621 accgtttgcg cgacatgttg caggcgcgtg aacccatcac gatacgcaac ttcgaaggcg
     4681 aaggctttaa tgaaatcatg aaacattttt acgtggaacg taacggcgta aaaaaagtgg
     4741 tcacccaagt cgaatttgca ggtcagtcgt atgaaatcga tatcgtcaat caatttgtgg
     4801 atgagtttaa tcgcgatgaa cagattcgta atgaaaacgt tatcatcacg ggtatccgcg
     4861 tcgtgacggc tgaatttctc accctcgtga aaaaggattt tcaagttgcc gtgatagctt
     4921 cattgtcggc gatattgcta ttagtgattg tgcattatcg caaatttcgc gcggtactcg
     4981 tatgcatggt gccccttacg tttgcattgc tcacgatcat gggcgtgatg cggctcatgg
     5041 gcatcaagat caattttgtc aatatgatca cgctcccgct tttgatcggg agcggcgtgg
     5101 actatggtat ttatattatt tcgcggtatc ttgaagatca aagccacgat gtttatgcgg
     5161 ccattcacga aaccggccag tcactttttc tgagtgcggc aactacggtg atcggttttg
     5221 gttcattgat tttcgtggac aatcgtgggt tggccagctt gggttatatg tgctcgtttg
     5281 gtattattat ctgttccgta tcgtccgtga ttatcctacc ggcgttattg cgtctgtggg
     5341 gtaaaaaaat atggaaggaa gcacgtgagg aagaatcgcc gaataaggac acgatgatca
     5401 aaaaagtaac agcggcgcat acgccggaaa aaaaatgaca cgcattttga ttgttatgct
     5461 ggcgtttgtc ggttgtacgc ctcaaccgat ctatcgtgcc gtgaaagccg atggacatgg
     5521 gccgtcggtg aaagcgactg aaacgcataa gccgactgca tcggtaagtg actcatccgc
     5581 gaccggtgca aagaaggaac cggcaaacac cgaagaagcg atgatggcgg agatcaaaac
     5641 ttggataggt acgccttacc gttttggtat ggaaaaaaaa tcagagggta cagattgctc
     5701 aggatttgtc ggtgcggttt tcagaaaagt tcgtcagatc gaattgccgc ggcaagctgc
     5761 ggacatgtat gccaaaggaa caccaatcac acgcagtgaa ttacgatttg gcgacttggt
     5821 ttttttccaa aatacctata agggcgccgt cggcgcttcg catgtaggta tctatgtcgg
     5881 aaataaccaa atcgctcatg ccggtacgac ggtcggcgta acgatatcat cactggagga
     5941 gtcgtactat gagaagcatt atttgggatg ccgcagaatt tcgcaatgat ttcgaaattg
     6001 atttgaggta actacgctga tgccgtttac aaaatattca cggcatcata aactctcttt
     6061 agtagtcttt aatttcaggt tttcggtttg agaagttgat tgagcacgcg ggatagatcg
     6121 ttgatgaaat agggtttggg caggatgcct tggaacccgt agcgtgtatg atcagccatg
     6181 acagggtcat tggaatagcc gctggaaaca atgccgcaga cagccggatc aatggacaaa
     6241 atccgcgcca tcgcatcttt accgcctaac cctcccggaa tcgtgagatc cataataatc
     6301 gcgtcaaaag ccttattacg tgcgcgtgca tcttcataca tcgcgacggc ttcttcgccc
     6361 gatttggctg agacggcatc aaatcccaag tggtttagca tattggaggc catttcacga
     6421 atagactctt cgtcgtccat caccaaaatc cggccattgg ataaaagcgc ttgcggctcc
     6481 tgcgggcgcg gctgctgaac ggcaaacttg gatgccggaa gatacagcgt gaacgtcgta
     6541 cccacattga ttttggagtc aaccgtgatc gcaccgtcgt gatttttgat gatcgagtac
     6601 gacgtcgcaa gacccaagcc gttgcctgtt tgtttagtag taaaataagg atcaaaaata
     6661 cgctgcagat gctcttcgga aattccgcaa ccgttgtctt caatagctat tttgaggtaa
     6721 gagccgggtt tgagtggcat gccttcattg tgggattgga tggcatggtt ttgtgcaaga
     6781 atacggataa tgccgccatt gggcatggct tgacgcgcat tgatgatcaa gttattgagc
     6841 acctggctga tctgaccggc atccacatcg gaaggataca gattatcttg aataaaaaat
     6901 tcgcatcgaa cattggaacc ggctgacgaa aattgtgcgg attcccgtaa cagatcggcg
     6961 atattggcgg tttttttgac cggtgcgccg cctttggaaa aagtgagcaa ttgttgtgta
     7021 agttctttgg cacgttcgga tgcgcgttcg gcgcggctca gaagttcctg agccttatct
     7081 ttttgtgcgg ccgcgatttt gactttcgcc aaagaaatat tacccataat ggccgaaaga
     7141 atattattga agtcgtgcgc aataccgccg gccagtacgc cgatggattc gattttgcgc
     7201 gatttcagca gttcctgttc cattttgatg cggtcggtaa tatcggaaaa aaccatcacg
     7261 acgccctgca tcgcaccgct catatcgcga atcggtgaag cgccggctaa aatagaacgc
     7321 ggcgactgat cgccggcaac tttataggta aattcggacg agacgaaggc ttgctgctcg
     7381 ttcaaaacga catccaccgg gtgtgtcgtt ttgttt
//
LOCUS       DAOQGF010000140         7413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4853_length_7413_cov_6.230090, whole genome shotgun sequence.
ACCESSION   DAOQGF010000140 DAOQGF010000000
VERSION     DAOQGF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_7413_cov_6.230090"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..442
                     /locus_tag="PK937_13995"
     CDS             89..442
                     /locus_tag="PK937_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33734.1"
                     /translation="MILLFIEDILFLSKIKTQARALGIQTVVADKANENVRELFLAQS
                     YRKIFVDLNIQKRDPFALIRLLKADSVAMSIPIIAFFSHVQTELAHKASEAGCDTVMP
                     RSQFSARLNDLLTGV"
     gene            439..1125
                     /locus_tag="PK937_14000"
     CDS             439..1125
                     /locus_tag="PK937_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNH33735.1"
                     /translation="MMLASLRLGLYWLADFLYPSTCLACRVRLDPADKHICKSCWNAL
                     KTVGESDYEGIIRCDAIDVIHAGYYYDEVLQKSIHALKYDRALSLIPKFADRLAQIVH
                     TNPMLKTATRITAIPLNPIRKRERGYNQAELLARAVGQLAGLSYVETLQRVRHTESQT
                     KMNTAEDRIKNVDGVFIAMADASVQDQSVILVDDIITTGSTANACAAALKKAGASKVF
                     VLTVGRPTLG"
     gene            complement(1287..3215)
                     /locus_tag="PK937_14005"
     CDS             complement(1287..3215)
                     /locus_tag="PK937_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNH33736.1"
                     /translation="MLQLVNLTVEFGGRTLFSNLTWKINDRDRVALIGPNGAGKSTLL
                     KIIARQQSATSGTIAQPKHLRVGYLPQDGLHAHGKPLLEEVVSAFDEIVRIKQRLDEL
                     EHALHTLDHEAPAYARALDEYGHLHQRLDDMGAASAESKAAKILNGLGFKESEFDKLC
                     ELFSGGWQMRIALAKLLLQNPDVLLLDEPTNHLDIDSIEWLEEYIKNYEGAIFMVSHD
                     RYFIDNVCNRIVELERQTLTDYVGSYDDYEEQKALAEEQLLAAYERQQDEIKRVQLFI
                     DRFRYKATKARQAQSRVKMLERMDRIQLPEEEKRAIQFRFPQPIKSGRVVLEIAGIQK
                     SYDGRPILKGIEFAIERGEKVALVGPNGAGKSTLLRIIAGDETPDTGTIKHGYNVTME
                     YFAQQQSDKLDMTRSVYEEIASAATNQPPLLLRTILGAFLFSGDDINKKVKVLSGGER
                     SRLAFAKMLLNPANFIILDEPTNHIDAQSKEILQGALTDYEGTLLIVSHDRYFLESLV
                     TKVIEVREGHAKIYPGTFAEYHERKIKEHAEAAARESIAEVKVKPIASKPEREVKKEP
                     VKADQQAKRKREKQLSQIESDIQKAETQKIEIEALLTDPLLYKNPDRQKTVQSEYNAL
                     HARITALYEEWNALAAEL"
     gene            complement(3221..4330)
                     /locus_tag="PK937_14010"
     CDS             complement(3221..4330)
                     /locus_tag="PK937_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNH33737.1"
                     /translation="MHATTDDRKSYNRQKLWLSLLSGVLSFCVLIALFFSGLSDTLAR
                     WVMSQTERPYLQFLLYLFALGLLQSIVTFPLSFYNGYILEHRYRLSNQSFKQWCFENV
                     KGLALGIVIGVPLLLIFFYTLRNFGFWWWLPLAGILFLFSVLLARIAPTLIFPLFYTF
                     KPVEKTALVERITRLCLKAGLQVQGVFEFNLSKTTKKANAAFAGIGKSKRILLGDTLL
                     QQFSDDEIEVVFAHEAGHYAHRHITKGMIWGIVSTFGGLCLTAFIYDNLFPAFEYTDR
                     AELGALPLIAILLSLYSVITTPAGHILSRRHEREADAYALRETKNRAAFISAMQRLSD
                     TNLADENPHPLVEWYFYSHPAIGKRIAAAETMILE"
     gene            4504..5586
                     /locus_tag="PK937_14015"
     CDS             4504..5586
                     /locus_tag="PK937_14015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH33738.1"
                     /translation="MRFVFPGLPVKNNADLMTQLNRGAERVAERLKTTDWSAVPVSDY
                     NKRYLKKYTAKLTPTFQKYVYILALALEKQNDLARCTLVDFGGGCGLLSCIAKAAGVG
                     RVVYNDIFETSVQDVRKLSEIWRIPINDFVCGDLDTVHQFLEREKINCDAFVSCDVLE
                     HIYRHELFFKQAAELPSKAMSLVMSTHANPLNPAIRRSLQASQRRVELEDRKAEWGHK
                     DRDDLRSYRRIRAEMISAENADLEKEMIYELAERTRGMKAEDIRATVKIFKTEGRMPP
                     KPDHPTNTCDPGNGNWADRLLEPQVYAQALKEAGFQTTVYSGFYGHPHPTGRRAIARL
                     LDAGIRWLGHHQGVRLASYFIVKGVK"
     gene            5713..6498
                     /locus_tag="PK937_14020"
     CDS             5713..6498
                     /locus_tag="PK937_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33739.1"
                     /translation="MATEPTFDLYLFSTHEETVKSALQAGVKGIGINWEHIGKSSTDG
                     TVTHDSIDDLTKIRAITSAPILCIINRFSPGTEREVEEAIKGGATEILLPMVTESSQI
                     EKTLQWANGRVGVGIVIETQAALRSARQLARYPLSRVFIGLKDLAEENRRTGKNTHIY
                     EPIVDGTIERVRSGFKMPVGFGELTLPECGTPIPSRLLMSEMARLQCSYGVLKQAFLQ
                     DIKDKSMADEVPRIIRAIQDTFAYSQTVFGLNRRALTGMLSQK"
     gene            6507..6878
                     /locus_tag="PK937_14025"
     CDS             6507..6878
                     /locus_tag="PK937_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011705627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH33740.1"
                     /translation="MNHSPGFLKVVNDSKTRVREISVQQCLDDIQKNPAIALIDVREE
                     SEYAAGHVSGAEHIGRGIIERDIETKHPDHDQVLYLYCGGGFRSALAADNLQKMGYTR
                     VYSVAGGWRAFLDIQAPVEKP"
     gene            6959..7393
                     /locus_tag="PK937_14030"
     CDS             6959..7393
                     /locus_tag="PK937_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HNH33741.1"
                     /translation="MAEQNYANHKQYRPLYHGFTFALILVIGALSIHKFVSAYRENSD
                     WTPSLLFVLIWIVLVFIFFYARIFALRAQDRAIRAEENFRHFILTGKPLDRRLVIAQI
                     IALRFSSDSEFPELARKAADENTDPDTIKKSIQNWRADHLRA"
BASE COUNT         1977 a         1645 c         1786 g         2005 t
ORIGIN      
        1 aagcgcacgg cggtaaaata tgggccgaaa gcgaattagg taaaggcagc actttcaagt
       61 ttttcattcc ttcatccgcg ccatccgcat gattttgctt ttcatcgaag acatcctttt
      121 tttgtccaaa ataaaaacgc aagcccgtgc gctaggtatt caaacggtcg ttgcggataa
      181 ggcgaatgaa aatgtccgag agttattttt ggcgcagagc tatcgtaaaa tttttgtaga
      241 tctcaatatc caaaaaagag acccgttcgc cctgatccgt ttactcaagg cggattcggt
      301 ggctatgtct attcctatca ttgctttttt ttcgcatgtg caaactgaat tggcacataa
      361 ggcgtccgaa gccggttgcg atacggtaat gccgcgctcc caattttctg cacgtcttaa
      421 cgacttactt acaggtgtat gatgttagcc tcgttacgtc ttggcttata ttggttggcg
      481 gattttcttt atccgtcaac ttgcttggct tgccgagtac gtttggatcc tgccgacaag
      541 catatttgta agtcatgctg gaacgctctc aaaacggtcg gcgaaagcga ttacgaagga
      601 ataatccgat gcgacgcgat cgatgtgatc catgcgggat attactacga tgaggtattg
      661 cagaaatcta tccatgcttt gaaatatgat cgtgcgttat cgctgattcc aaaatttgct
      721 gaccgattgg cgcagatagt gcatactaac ccgatgttaa aaacagccac acggattacg
      781 gccatacctt tgaaccccat ccgtaaacgt gaacgcggat acaatcaggc cgaattacta
      841 gcccgtgccg tagggcaatt ggccggttta tcctatgtag aaactttgca gcgcgttcgg
      901 cacaccgagt cacaaacgaa aatgaatacg gcggaagatc gtatcaaaaa tgtagacggg
      961 gtttttatag cgatggccga tgcgagcgta caagatcaat ccgtgatcct cgtggatgat
     1021 attattacga caggatccac ggcaaatgct tgcgctgcgg ctttaaaaaa agccggtgct
     1081 tcaaaagtat ttgttcttac cgtaggtcgt cccaccttgg gttaatgccc gttttatcct
     1141 gatcgcaact ttttttttga gattacgtat tgggagagta ctgtacggtc aaacgtatgg
     1201 ttttcctctc ctcctcatag taaagccccg ataataccat gtattacggg gctttgactt
     1261 ttaaaaagtt ttcagaccga aaacaatcac agctcggcag cgagggcatt ccactcttca
     1321 tacaacgccg tgatacgtgc atgcaaagca ttgtattcgg attggaccgt tttttgacga
     1381 tccgggtttt tatatagcag gggatcggtc aacagagctt cgatttctat tttttgggtt
     1441 tcggcttttt gaatatcgct ttcgatttgg ctcagttgtt tttcgcgttt acgcttggct
     1501 tgttggtcag ctttgaccgg ttctttttta acctcacgtt cgggctttga ggcaatcggt
     1561 tttactttta cttcagcgat agattcgcgt gcagcggctt cggcgtgctc tttgattttg
     1621 cgctcatgat attcagcaaa tgtaccgggg taaattttag catgcccttc gcgcacctct
     1681 atgacctttg tgacaaggct ttctaaaaaa tagcgatcgt gggagacgat gagcaatgtg
     1741 ccttcataat cagtgagcgc accctgtaga atttctttgg attgtgcgtc aatgtgattg
     1801 gtcggttcat ccaaaatgat aaaatttgcc ggattgagta gcattttggc aaaggcgaga
     1861 cgcgagcgtt ccccaccgct gagtactttg acttttttat tgatatcatc gccggaaaaa
     1921 aggaaggcac ctaagatggt acgcagtaat agcggcggct gattcgttgc cgctgaagcg
     1981 atttcttcat acacgctgcg ggtcatatcc agtttatcgg actgctgctg tgcaaaatat
     2041 tccatcgtaa cattgtaacc gtgcttgatc gtaccggtat ccggcgtttc atcgcctgcg
     2101 atgatacgca acagcgttga tttaccggcg ccgttaggac cgacaagggc tactttttcg
     2161 ccgcgctcaa tagcaaattc gatacctttg agaatcggtc gtccgtcgta cgatttttga
     2221 attccagcga tctcaagcac gacccgtccg cttttgatcg gttgaggaaa ccgaaactgg
     2281 atggcccgtt tttcttcctc aggcaattga atgcggtcca tgcgttccag catcttgacg
     2341 cgactctgcg cctggcgggc tttggtcgct ttataacgga agcgatcaat aaaaagctga
     2401 acacgtttta tttcatcctg ctggcgttcg tacgccgcga gaagttgttc ttcagccaat
     2461 gctttctgtt cttcatagtc gtcgtaagaa cctacataat cggtcaacgt ttgacgttcg
     2521 agttcgacga tacggttgca aacattgtct ataaaataac gatcatgcga caccataaaa
     2581 atggcgcctt cataattttt gatgtattcc tccagccact cgatggagtc aatgtccaaa
     2641 tggtttgtcg gttcgtcaag caataataca tcgggatttt gcaaaagaag cttagctaag
     2701 gcgatacgca tttgccagcc gccgctaaaa agttcgcaca gtttatcaaa ttcgctttct
     2761 ttaaaaccga gaccgttcag aattttggcg gctttagatt cggcggaggc cgcgcccata
     2821 tcatctaagc gctgatgcag atgcccgtat tcgtccaatg ctctggcata ggccggtgcc
     2881 tcgtgatcca gagtgtgcaa tgcatgttcc aattcgtcga gcctttgttt gatccgaacg
     2941 atttcatcaa atgccgatac gacttcttcg agaagcggct taccgtgggc atgcaatccg
     3001 tcctggggca aatatccgac gcgcaaatgt ttgggttggg cgatggtgcc gcttgttgcc
     3061 gattgctgac gcgcgatgat tttgagaagt gtggattttc cggccccatt gggaccgatc
     3121 aaagccacgc ggtcgcgatc attgattttc catgtgaggt tagaaaataa cgtgcgtccg
     3181 ccaaattcga ccgtaagatt gacaagctgt aacatagatg ctattccaat atcatcgttt
     3241 ccgcggcggc aatgcgtttg ccgattgcgg gatgtgaata aaaataccat tccaccagcg
     3301 gatgcggatt ctcatccgca agattggtat ccgacaaacg ctgcatggct gaaataaaag
     3361 ccgcacggtt tttggtttca cgcagcgcat acgcgtccgc ttcgcgttca tgccgacgac
     3421 tgagaatatg tccggccggt gtagtgataa cactgtataa actcagcaga atggcaatga
     3481 gaggcaatgc gcctaattcc gcacgatccg tatattcaaa agcaggaaat aaattatcat
     3541 atatgaaagc tgtcaaacag agtccgccga atgtagaaac gatgccccag atcatgcctt
     3601 tggtaatgtg gcggtgcgca tagtgaccgg cttcgtgcgc gaaaacaact tcgatttcat
     3661 cgtcagaaaa ttgttgtaac agcgtatcgc ccagcaaaat acgtttggat ttaccgatac
     3721 cggcaaacgc cgcgttggct tttttggtag ttttactaag attaaattcg aataccccct
     3781 ggacttgcaa accggctttt aagcacaagc gggtgatgcg ctcgacgagc gctgtttttt
     3841 cgaccggttt aaacgtatag aacaagggaa aaatcaacgt cggggcgatc cgtgctaaca
     3901 aaacggagaa taaaaacaat atgccggcca aaggcagcca ccaccaaaat ccaaagttgc
     3961 ggagcgtgta aaaaaaaatt aaaagtaaag ggacgccgat cacgatgccc agcgccaagc
     4021 cttttacatt ttcaaaacac cattgtttga atgactgatt ggataaacgg tatcggtgct
     4081 ctaaaatata tccgttataa aaactcaaag gaaaagtgac gatgctttgc aataaaccca
     4141 aagcaaatag atacagtaga aattgtaaat aaggtcgttc cgtttgcgac ataacccatc
     4201 gggccaacgt atctgacaaa ccggaaaaaa aaagcgcgat taatacacaa aaactcaata
     4261 caccggatag taggctgagc cacagttttt ggcggttgta ggattttcga tcgtccgtcg
     4321 tcgcatgcat atagttaatt gctccgttta tgcgcgcgca atttatcaaa aacagccata
     4381 cttacaaata taatttgcat ccatcattca taacgacgta gtgaaatacc ttcttgcgaa
     4441 tcacaccatt ttctgcgaca ttaattacca ttgacgattt ttgaaaaaag gaatatctga
     4501 aatatgcgat ttgtttttcc gggattgccg gttaagaata acgccgacct aatgacgcaa
     4561 ctcaaccgcg gcgcggaaag ggtagccgag cgactgaaaa cgacggattg gtcggccgtg
     4621 cccgtgtcgg actacaataa aaggtatcta aaaaaatata ctgcaaaatt gacgccgacg
     4681 tttcagaaat atgtgtatat acttgcactc gcattggaga aacagaacga tctcgcacgt
     4741 tgtacattgg ttgatttcgg cgggggatgc ggtttgcttt cgtgcatagc caaagcagcc
     4801 ggcgtggggc gcgtcgtcta taatgatatt tttgaaacat ccgtacaaga cgtacgaaaa
     4861 ctttcggaga tttggcgaat cccgatcaat gattttgtgt gcggcgatct ggatacggtt
     4921 catcagtttc ttgagcgcga aaaaataaat tgcgatgcat ttgtatcatg cgatgtgttg
     4981 gaacacattt atcggcatga actatttttc aaacaagccg cggagttgcc ttccaaagcg
     5041 atgtcattgg tcatgtcaac gcatgccaat cccctcaatc cggctattcg ccgttcactg
     5101 caagccagtc agcgtcgtgt cgaactcgaa gatcgcaaag ccgagtgggg ccacaaagat
     5161 cgggatgatt tacgttccta tcgtcgcatc agagccgaaa tgatcagcgc tgaaaatgcg
     5221 gatttggaga aagaaatgat atatgaatta gctgagcgta cacgcggtat gaaagcagag
     5281 gacatccgcg caacggtgaa aatttttaaa acagaaggcc gtatgccgcc taaaccggac
     5341 catcctacca atacgtgcga tccgggcaat ggaaactggg cagaccgact tttggaaccg
     5401 caggtatatg ctcaagcctt gaaagaagcg ggatttcaaa cgacggttta ctccggtttt
     5461 tatggtcatc cgcatccgac aggacgtcgc gcgatagcgc gattattaga cgccggtatt
     5521 cgttggctgg ggcatcatca gggcgtgcga ctcgcatcct attttatcgt taaaggcgtt
     5581 aaataaacga cgcttgcggt ttttttatat ttatacgata ttcgctttac ttatctgtaa
     5641 tattcttatt actatcataa acataaagct tatcaaaact attcgaaata agttcgcgta
     5701 acgggagtgt gcatggcgac ggaaccgacg tttgatttgt atcttttttc tacgcatgaa
     5761 gaaactgtaa aatcagcgct gcaagccggc gtgaaaggta tcggtatcaa ttgggagcat
     5821 atcggtaaaa gctctacgga tggtactgtc acgcatgata gcattgacga tttaactaaa
     5881 ataagagcta tcacctcggc gccgatcctc tgcatcatca accgttttag ccccgggacg
     5941 gaacgcgagg tcgaggaggc gatcaaaggc ggcgcgacgg agattttact acccatggta
     6001 accgaaagca gtcaaatcga aaaaacactg cagtgggcta acgggcgcgt gggtgtgggt
     6061 atcgttatcg aaacgcaagc cgctttgcgt agtgcacggc agttggcgcg ttatccgctg
     6121 agccgcgtat ttattggtct taaagatttg gcggaagaga accgtcgtac cggaaaaaat
     6181 acgcatatct acgaaccgat cgtggacggc acgatcgaac gggtacgtag cggtttcaaa
     6241 atgccggttg gtttcggtga acttacatta cccgaatgcg gcacaccgat tcccagtcgt
     6301 cttttgatga gcgagatggc gcgtttgcaa tgttcgtacg gcgttctcaa acaggctttt
     6361 ctacaggata ttaaagataa atctatggcc gacgaagtgc cgcgcattat tcgcgcgata
     6421 caggatacct ttgcgtattc acaaaccgtc tttgggctca atcgtcgcgc attgaccggt
     6481 atgttatctc aaaaataaga ggtcgtatga accacagtcc gggttttcta aaagttgtaa
     6541 atgattccaa aacacgagtg cgcgaaattt cggtacagca gtgcctggac gatattcaaa
     6601 aaaatcccgc gatagcattg atcgatgtgc gtgaagaaag cgaatatgcg gcgggtcatg
     6661 tttccggtgc ggaacatatc gggcgcggta ttatcgaaag agatatcgaa accaaacacc
     6721 cggatcacga tcaagtattg tatctctatt gcggcggtgg ttttcgttct gcgctggcag
     6781 cggataatct gcaaaaaatg ggttatacgc gcgtgtattc cgtagccggc ggatggcgtg
     6841 catttttaga tatccaggca ccggtcgaaa aaccataaat taagttgcag ttgcattttt
     6901 gatcggacgt tgcgtattat caaataaggt tctcctaaga cataagaaat cggaaaatat
     6961 ggctgaacag aattacgcca accacaagca gtaccgtcct ttgtatcacg ggtttacgtt
     7021 tgccttgata ctggtgatcg gtgcgctttc aattcacaaa ttcgtatccg cgtatcgaga
     7081 aaactcagat tggacgccgt cattgctttt tgttttgata tggatcgtgt tggtctttat
     7141 ttttttctat gcgcgtattt ttgcacttcg ggcgcaggat cgtgcgatac gtgccgagga
     7201 aaattttcgt cattttattt tgaccggaaa accattggat cggagattag ttatcgctca
     7261 gattattgcg ctacggttta gctcggatag tgaatttccg gaacttgcgc gtaaagcggc
     7321 ggatgaaaat acggacccgg acacaataaa gaaatccata caaaactggc gcgcagatca
     7381 tttgcgagct tgaggaatat attatgaaaa ata
//
LOCUS       DAOQGF010000141         7361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4916_length_7361_cov_6.128251, whole genome shotgun sequence.
ACCESSION   DAOQGF010000141 DAOQGF010000000
VERSION     DAOQGF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_7361_cov_6.128251"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..5795
                     /locus_tag="PK937_14035"
     CDS             72..5795
                     /locus_tag="PK937_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH33742.1"
                     /translation="MSSLTLQVHTTPYYWGYADDLSSAGVTVVDSQWYHLVFTYEGGV
                     GKFYINGQLNSSGALSPNTPDTLLWIGNFQTQEEFGNFKGKLDDILIYNRALLPNEID
                     SLYHDGIPTITSFTPTSGPIGTSVTITGTNFNTTAANNVVYFGGVKASVTNATSASLT
                     VTVPTGATYAPITVTDTTTRLSVASKLPFIVTFSGIRGVTSGSFASKVDFVTGTNPNP
                     LSIGDMDGDSKLDIVAINGGENSVSVFRNTSTTGTISTGSLASRVNFNTGNTPFEVAV
                     GDLDGDGLFDMAIVNPNSGTISLLRNTSTTGSITSGSFAAKVDLMAGNFPNGVAIGDI
                     DGDGKADLAVTNQNSNTVSIFRNISVKGSLSTSSFQTKLDFVTGSTPYGIAIYDLDMD
                     GKRDILFANSGDNSISICRNISTIGNITTGSFLSPINFATGANPQQISVSDIDGDEKP
                     EIIVSHFGGSTISLFQNTSSIGSITSESFAPKIEFTTGTGPYEISIADIDGDSKPDIA
                     VTNFNSNTVSILRNTSSVGSITASTLASKIDFSTGNSPNGVILGDIDGDGRVDVAVSN
                     YGASTISLLKNTTNDLVAYYPFTGGSTADSSGNGNNGTNNGATPTTDRFGNANGAYSF
                     NGTSNYVSTNLDVQPSAIPITSWSAWIYPTRLNYASNQAILSSDDGLADRSLTIRQTT
                     DSIAVYVGGGVEWRPFKLNANQWQHLVVTYKSDNIYVYLNGVKYTYGSAGNILTTVNK
                     LTVGKNPGLSNYFQGNIDDIVIFNRQLSDQEVDSLYHVGNWNSPPVVTSALRDTSLNE
                     DFGKVFIRRLNTVFTDVETSSLSDTAYVLSGSVTALIPSGTDSLYLQSVANYSGTASI
                     RIKATDGSNASVSDTFTVTINAVNDAPIRTAMIPDTTLSEDFMKTFRRKLTAHFTDVD
                     NGTLTYTTTVLSGSVTTSISNDSLFVNSVADYNGSATIKITATDAASASVTDTFVVMI
                     QPVNDPVQKLSALRDTTFIEDTPRHFAIRLSEHFNDMDSPVLTYQAIALDSGIRVFAQ
                     HDSLFVEGVPDSNGTKRISVSAGDGETIAKDTVNVSLTPQEDAPRLKLALTDYQMSED
                     SLSSSKRIAIELKSHFYDPDGDALTFSRNIADGRGGIQTALSNQTDTLFIWNVADSNG
                     TFKIAISADDDKGGQKARDTIILSVSPVNDAPLTSQPVADVFVNENFSKTAIAYLRST
                     FRDPDGDVLGYGSFTAGGYTDKVTLVTSGDTLYAISKPDSFGVYVGYAKAQDAVTSVA
                     DTFRITIKGRPRVTGIQSVSIDTTSFVVNASINPNGTISSLFVRYGRSVEAMNSVSDT
                     SSHSVVETAVHTLHNLIPDSTYFYRVISFSEAGFDSNSIPLSTRTLQVPSEFDQVTVS
                     DPNPSPGTTITITAKVLGSRADSVVLIYGLSKDTIGKRLRMSWNGGLLYSGTIPSASV
                     TINGLWYRVIAYNQRGPKSFPSQGRRQNISIQVSVAAISEIKMVSQFPAGLPENDWSI
                     VSLPVNGSLDLEKYFGKQDMKNGKPTNWSAGTWNTDSNKFISAKILEGGKGYILFHRS
                     KSKNFPFDSIANARTNAPDYFDSIVVHKGWNIIAWPYTYVDTISILAAGFVHNVWTIR
                     DSAGSKVEWTNNSFVMRPYSGFMIKLQNSYPQNTAYLGDLIRRNHKTTVVLSHPNWSI
                     QLAAENDHYRDSKNRIGISESASNGDDGMDGSKPPQFDDRVRLTVRRQEKSGLFENLS
                     SDIRTLTDSGHVWDFEIANAGTGSRFYWISTGIPDNLDAVIVDLTANKAYDIRGNNTV
                     PLIHAEHQFKLIAGNSSFVDQKMKALMETMPTTFSLKQNYPNPFNPTTRIDFDIAHTT
                     HVRIRIYNLLGQEVRTLQNSIMETGRHFVIWDGKDAHGRAVASGVYLYRLEAGAVTKV
                     KKMLLLK"
     gene            6157..6726
                     /gene="gmk"
                     /locus_tag="PK937_14040"
     CDS             6157..6726
                     /gene="gmk"
                     /locus_tag="PK937_14040"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNH33743.1"
                     /translation="MANLSSSKGKLIVFSAPSGTGKTAIKNTLLKMDPTLVFSISATT
                     RAKREGENDGKDYYFISEEEFKDHIQNDDFVEYDHHFGNYYGTLRMAVEPDLRMGCNV
                     IMDLDVNGALNVKKSYGGTSVLIFIKPPSMDELRKRLMSRGTETEETLKIRLARVQEE
                     MDRAHLFDHVVVNDTIERAANEILDIIRR"
     gene            6728..>7361
                     /locus_tag="PK937_14045"
     CDS             6728..>7361
                     /locus_tag="PK937_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HNH33744.1"
                     /translation="MPADTAHNDQLRSFVLNSNETEIEKNLRSIGESIGLYGESETIR
                     TIIRDVCRVAPSDVAVLVIGESGTGKDVVAKAIHQLSARRNKPLVIVNSGAIAEGILE
                     SELFGHERGAFTGAVSERKGYFETANGGTIFLDEIGDMPLQTQVKLLRVLETGEFLKV
                     GGSVVRRCDVRVIGATNRNLEQMVRRGEFRKDLYYRLKAITITMPPLRQRR"
BASE COUNT         2105 a         1535 c         1726 g         1995 t
ORIGIN      
        1 gggtcaataa atctgggaat accgctaaac ctccgatgct cgtgggatgg gggtatcgtg
       61 gaagcaatct gatgagttca ttaacgctgc aagtgcatac aaccccatat tattggggat
      121 atgccgatga tctttcatct gctggcgtaa cggtcgttga ttcacagtgg taccatcttg
      181 tttttactta tgaaggcgga gtaggaaaat tttatattaa cggtcagttg aattcgagcg
      241 gagcattaag cccgaatact cctgatactt tgctttggat aggtaatttt caaactcagg
      301 aggaatttgg caatttcaag ggaaaacttg acgatatttt gatttacaat cgcgccctat
      361 taccgaatga aattgattca ctttatcacg atggtattcc cacgatcacc tccttcacgc
      421 caacctccgg ccctatcggc acaagcgtca cgatcacagg gacgaatttc aatacgaccg
      481 cggctaacaa tgtcgtttac ttcggcggag taaaagcgag tgtgactaat gcgacttccg
      541 cgagcctaac ggtgaccgtt cccaccggcg cgacatatgc gcctatcacg gtgacggata
      601 cgacgacgag attgagcgtg gcatccaagt taccttttat tgtaacattt tctggaattc
      661 gaggcgtaac atcgggttca tttgcatcaa aagtagattt tgtaactgga acaaacccaa
      721 atccattgtc tatcggtgat atggacggcg atagcaaact tgatattgta gctataaatg
      781 gaggtgaaaa tagtgtttca gtatttcgaa atacaagtac tacgggtact atttcaaccg
      841 gatcattagc ttcgagagta aatttcaata ctggaaatac tccatttgag gtggctgttg
      901 gagatttaga cggagatggc ttattcgata tggcaattgt aaacccaaac agtggaacga
      961 tttcgttgct acgaaataca agtacaactg gtagtattac atcgggttct tttgcagcta
     1021 aagtagattt gatggctggg aattttccga atggagttgc cataggagat atagatggag
     1081 atgggaaggc agatttggcc gtcacaaatc aaaacagcaa tacagtttca atttttagaa
     1141 atataagcgt caaaggaagc ctttcaacat cttcatttca aacaaaactg gattttgtca
     1201 ccggcagtac tccctatggc attgcaatat atgatctgga tatggacgga aaacgcgaca
     1261 tactatttgc aaattctggg gataattcga tttctatttg tcgaaacatt agtacgatag
     1321 gaaatatcac tacaggttcg tttttatctc caataaactt cgctacaggt gcaaatccgc
     1381 aacaaatttc cgtaagcgat attgacggcg atgagaagcc ggaaataatt gtctctcatt
     1441 ttggaggtag tacgatatct ctttttcaaa acacaagttc tatcggaagt ataacttcag
     1501 agtcttttgc gcctaagatt gaattcacta ctggtacggg cccatacgaa atttcaattg
     1561 ctgatattga tggtgactca aagcctgaca tagccgttac gaattttaat agcaacactg
     1621 tttcgatttt acggaataca agttcggttg gaagcattac tgcgagcact cttgcatcaa
     1681 agattgattt ttcaactgga aacagcccta atggagttat tcttggtgat atagatggcg
     1741 atggtagagt agatgttgca gtgtcaaatt atggggcaag tacaatttct ctattgaaaa
     1801 acactacaaa cgacctcgtt gcttactacc ccttcaccgg cggcagcacc gctgactcca
     1861 gcggaaatgg aaataatggt accaataacg gtgcgacgcc gacgacggat agattcggga
     1921 atgcaaatgg ggcgtatagt tttaatggaa caagtaatta tgtttcgacc aatttagatg
     1981 ttcaaccgtc cgcgattccg attacatctt ggtcggcatg gatttaccct acaagactga
     2041 actatgcaag taatcaggca atcctgagca gtgatgacgg attagcggat cgatcgttaa
     2101 cgatcagaca aacaacggat tccatagcag tttatgtggg tggtggtgtt gagtggcgac
     2161 cattcaaact gaatgcaaat caatggcaac atttggtcgt cacctataaa tcagacaata
     2221 tttatgttta tctcaatggt gtgaagtaca cttatggttc tgccggaaat attcttacta
     2281 ccgttaacaa gttaaccgtt ggaaaaaatc cgggtttatc caattatttt cagggtaata
     2341 ttgatgacat tgtgatcttc aatcgtcaac tttccgacca agaagttgat tcgctttatc
     2401 atgttggaaa ttggaatagt cctccggtag ttacatctgc attgcgagac acttcgctta
     2461 atgaagactt tgggaaagta ttcatacgca gactcaatac ggtgtttaca gatgtagaaa
     2521 cttccagtct gagtgacaca gcgtatgtgt tatccggttc ggtgacggcg ctgattcctt
     2581 cgggtacgga tagtttatat ttgcagagcg ttgcgaacta tagcggcaca gccagcatac
     2641 gaatcaaggc gacggatgga tcgaatgcat cggtatcgga taccttcacg gttacgatca
     2701 atgccgtgaa tgacgcgccc atacgcacag cgatgatacc ggatacgaca ctgagtgaag
     2761 actttatgaa aacatttcgt cgtaaactga cggcgcactt cacagacgtg gacaacggaa
     2821 ctttgacgta tacaaccacg gtattgtccg gtagtgttac aacatcaata tccaatgata
     2881 gtttgtttgt gaatagtgtg gccgactaca acggctcagc caccatcaag attacagcaa
     2941 cggatgcggc gagtgcatca gttacagata cctttgtggt catgatacag ccggtcaatg
     3001 atccggtaca aaaattgtct gcattacggg ataccacctt tatcgaggat acgccacgtc
     3061 attttgctat ccgtttgtcg gagcatttca atgacatgga cagcccggtg ctaacctatc
     3121 aagctattgc tttggattca ggaatccggg ttttcgctca acacgattct ttgtttgtgg
     3181 aaggtgtacc ggactccaat ggaacgaaac gcattagcgt ctcggctgga gatggtgaga
     3241 cgatagcaaa agatacggta aatgtttcac ttacgccgca agaggatgca ccacgattga
     3301 aactggcgct aacggattat caaatgagtg aggatagtct tagctcaagc aaaagaatcg
     3361 ccatagaact taaatcacat ttctacgatc cggatggcga cgcattgact tttagtcgaa
     3421 atatcgcaga tgggcgagga ggtattcaaa cggcgctttc taatcaaacg gatacgctgt
     3481 tcatatggaa tgttgccgat tcaaatggta cgttcaaaat tgcaatatca gccgatgatg
     3541 acaagggggg gcaaaaagca agagacacca ttattctttc ggtatctccg gtcaatgatg
     3601 cgcctttaac ttcacaaccg gtggccgatg tttttgtaaa cgaaaacttc tcaaaaaccg
     3661 caattgccta tctgcgcagt acattcagag atcccgatgg cgacgtgctc ggttatggca
     3721 gttttaccgc tggcggatat acagacaaag tcacgctcgt gacttccggc gatacgcttt
     3781 atgcgatcag caaacctgat tcattcgggg tgtatgtggg ttatgccaaa gctcaggatg
     3841 ccgttacttc ggttgcagat actttccgta taacgatcaa aggccgtccg cgcgtgacgg
     3901 gtattcaatc cgttagtatc gacacgactt catttgtagt gaatgcaagt atcaacccca
     3961 atggtacgat atcatcgcta tttgtgcgtt acgggcgatc tgtggaagcg atgaatagtg
     4021 tcagtgatac gtcttcacat agtgtcgttg agacggcagt gcatacactg cacaacctga
     4081 taccggattc gacatacttc tatcgcgtca tttcattcag tgaagctggt tttgattcga
     4141 actcgatacc actcagcacg cgaacgcttc aggttccgtc agaatttgat caagtgacgg
     4201 tttccgatcc caatccttcg cccggtacga cgatcacaat aacagctaaa gtattgggta
     4261 gtcgcgctga ctctgttgtt ttgatttacg ggcttagcaa agatacaatc ggtaaacgcc
     4321 tgagaatgtc atggaatggt ggtcttttgt attccggcac aattccctcg gcaagtgtta
     4381 cgatcaatgg tctttggtat cgcgtgatcg cctataatca gcgcggaccc aaaagttttc
     4441 catcgcaagg ccgacgtcaa aatatttcaa tacaagtttc tgtagcggcg atatcagaaa
     4501 tcaaaatggt tagtcagttt ccagcggggc tgccggaaaa cgactggagt atcgtttcgc
     4561 ttccggtaaa cggatctctg gatttggaga agtacttcgg aaaacaagat atgaaaaatg
     4621 gcaaaccgac caactggagt gccggcacgt ggaataccga tagcaacaaa tttatttctg
     4681 caaaaattct tgaaggcggc aaaggttata ttttatttca tcgttcaaaa tcgaagaatt
     4741 tcccatttga ttctattgcc aacgcaagaa ctaatgcgcc tgactatttc gacagcattg
     4801 ttgttcataa aggttggaat atcattgctt ggccttatac gtatgttgat accatatcca
     4861 tcttagccgc cgggtttgtt cacaatgtat ggacgatccg cgattccgca ggatcaaaag
     4921 tcgaatggac caataactcc ttcgttatgc gtccgtatag tggcttcatg atcaagttgc
     4981 aaaacagtta tccgcaaaat acagcgtatc taggtgacct gattcggaga aaccataaaa
     5041 ccaccgtcgt attgtcacat cccaattggt ctatccagct agctgccgaa aatgatcatt
     5101 atcgcgacag caagaaccgt attggtataa gcgaaagcgc atctaacggt gacgatggca
     5161 tggatggcag taagccaccg caattcgatg atcgagtccg actgactgtt cggcgtcaag
     5221 aaaagtcggg attgtttgaa aacctatcca gtgatattcg gacgttgacc gattcaggtc
     5281 atgtgtggga ctttgagatt gcaaatgcgg gtacaggttc cagattctac tggatcagta
     5341 cgggaattcc tgacaatctt gatgctgtga ttgttgattt gactgcaaat aaggcctatg
     5401 acatccgagg caataacacc gttcctctta tccatgctga acatcaattc aaattaattg
     5461 caggaaatag cagttttgtt gatcaaaaaa tgaaagcgtt gatggaaaca atgccgacga
     5521 cattcagcct aaaacaaaac tatcccaatc cgtttaatcc gacgacgcga attgattttg
     5581 atattgcgca tacgactcat gtccgaattc gcatatataa tctgttaggg caagaagttc
     5641 gaacactaca gaatagtatt atggaaaccg gccgtcactt tgtcatctgg gatgggaaag
     5701 atgcgcatgg ccgagcggta gccagtggcg tttatctata tcgcctcgaa gcgggtgctg
     5761 tcacgaaagt aaaaaagatg ttattgctaa aataaaaacg aataaaagac aaatagagat
     5821 catttcgaca agtgaagtgg gggagaactg ctcgctcatt cgcaaagcag ataaacggat
     5881 agatttttcc gcgtctttgt gagaatgaat cctgttacta tttcgaccaa cggatcgtgt
     5941 ggaaaacttc gaacgggttt gctatctgta ttcctaattg aattgaccct ttctaattat
     6001 cctataaaaa gtaaactgcc cgaagggaaa atattatttc cctgtcttga caatcgattc
     6061 acattttacc aatttatttc ttgccttaaa cttaagtaaa atctatattt ccgcttattg
     6121 taaagtaata aactgtaaaa atgtcagtac ttaaaaatgg ctaatctttc atcatccaaa
     6181 ggcaagctga tcgtctttag tgctccatcg ggtacaggaa agaccgccat taagaatacc
     6241 ttgctcaaga tggacccgac gttggtattt tccatttcgg cgacgacacg tgccaaacgt
     6301 gaaggtgaga atgacggcaa agattattac tttatttcag aagaggaatt taaagatcac
     6361 atacagaatg atgattttgt ggaatacgac caccatttcg gaaattatta cgggacattg
     6421 cgtatggccg tcgaaccgga tctccgaatg ggttgtaacg tgatcatgga tctggatgtc
     6481 aatggcgcgc tcaacgtaaa aaaatcatac ggcgggacct cggtacttat atttattaaa
     6541 ccgccttcta tggatgagct gcgtaagcgt ctcatgtccc gtggtacaga aacagaagaa
     6601 acgttgaaaa tacgcttggc gcgcgtgcaa gaggaaatgg atcgcgcgca tttatttgat
     6661 cacgttgtgg tcaatgatac catcgaacgg gctgccaacg aaattttgga tatcatccgt
     6721 cgctgatatg ccagctgata ccgcacataa cgatcaactc cgcagttttg ttctgaacag
     6781 taacgaaacg gagatcgaaa agaaccttcg ttcgatcggt gaaagcatag ggctttacgg
     6841 cgaatcagaa accatccgaa ccatcattcg ggatgtatgc cgggtcgctc cgtcggatgt
     6901 ggccgtgctg gtgatcggtg aaagcggcac aggtaaagac gtggtggcca aagccataca
     6961 tcagttgagt gcgcgtcgca ataaaccgct ggtgatcgtc aatagcggtg ccatcgcgga
     7021 gggtattttg gaaagcgaac tgtttggcca tgaacgcgga gcgtttacgg gtgcagtttc
     7081 cgagcgtaaa gggtattttg aaaccgccaa cggcggcaca atatttttgg atgaaatcgg
     7141 cgacatgccg cttcaaacgc aagtgaaact cttgcgcgtt cttgaaaccg gtgaattttt
     7201 aaaagtcggc ggttcggtcg ttcggcggtg cgatgtgcgt gtgatcggcg cgaccaatcg
     7261 caatttggaa caaatggtgc gccgtggtga atttcgcaaa gacttgtatt atcgtctcaa
     7321 agcgatcacc attacgatgc cgccgctacg tcagcggcgg g
//
LOCUS       DAOQGF010000142         7342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4932_length_7342_cov_7.638260, whole genome shotgun sequence.
ACCESSION   DAOQGF010000142 DAOQGF010000000
VERSION     DAOQGF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_7342_cov_7.638260"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1366
                     /locus_tag="PK937_14050"
     CDS             302..1366
                     /locus_tag="PK937_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33745.1"
                     /translation="MKLAKPLLALVYVLTVAVFIACSENNESNKHSNKFHKSQFLEKG
                     NLWKESEFESLLRQNPSKSEALNAVGRSLQVPGVGLVFDEEGYLYTPTQFNQLVEEIT
                     TSTGDDTGAVIDQLMDVYYTDLNTYADVTSDPNAVVENTIGIAPLDSSDEFTSPYYVL
                     DMYQEAYLPKESKLNKLKAEASLFMPKGIEKLSGTPNPPPGIVFPADVVIADRKGSAF
                     GHAGVVIIPGFGNSLQGAMMMEAVGNRKHKADEVVSRPSDKYFFRSDLTYMKICYREN
                     ITIQQRSDITKYVVAQDPDPYSIWTSKDTGGKWYCSKLPWRAYKLKVGCNIDEDGGFW
                     VTPFDIAGDASLKGSYYWHN"
     gene            1438..2508
                     /locus_tag="PK937_14055"
     CDS             1438..2508
                     /locus_tag="PK937_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33746.1"
                     /translation="MRIIWLLFSLCYYGCSTDQKDLYLHTGDKLDSEQFYTGNLTSQE
                     YSECATMIIPGGFAIIKPDTIPTLIKTIQVPPIIYSAQMDQNGLVWVAPPYNQEDDNS
                     ERIYIIDPVTERVVKSITLPQELRTPSCLKISGNKVYFRGDRNGFSVGIGAIDRLTYN
                     NIEAIAKFDTTGFASSPGLEIINNNILAFCETNGRQSSKIVLFNPVTKTIITKVIGNY
                     YRYTYDLNYIYLTVQTAENQIILQKIDPSNLNLLTEVLLGGDEIYITSNQNNICVGSF
                     ANKYIKIVEKSNLTILKTIDFSKQPGIYFNRSFGFITQRMLMLNDNSFYDMQNDIIHS
                     KLFPINYPQTMNLTLAEGQTLK"
     gene            2549..3007
                     /locus_tag="PK937_14060"
     CDS             2549..3007
                     /locus_tag="PK937_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33747.1"
                     /translation="MIKIVLVFICFFCSTATISAQVKTPNAVYVEVIGNGGFYSVNYE
                     RLFCNYFGFRVGASYLYLGDVGQYASFPILANFFMGGKHHKFELGVGCTVMNDAPVGE
                     FSNWDTKYFRTITLGYRYQSKENFFRIGFTPLFTGHEIIPLGGISFGRAF"
     gene            3285..3833
                     /locus_tag="PK937_14065"
     CDS             3285..3833
                     /locus_tag="PK937_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33748.1"
                     /translation="MPSQWFVGLDIGGGGVYWNLDDPSEPGSFNGAGGIFNIRTSINY
                     KMNRFSIGLGGKYDKIYVGELHNTINHDIVKKFDRGYIAQTKFFFIVEYDLFKRKNWS
                     IGSAISAGLAHTKAGLDGSLSKNNPYFGISTYLKWNDHHKLTYSIRPYAEIQSIKNHT
                     TSLQQHTVVDFGCAIGLQFVVF"
     gene            3864..4457
                     /locus_tag="PK937_14070"
     CDS             3864..4457
                     /locus_tag="PK937_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33749.1"
                     /translation="MPSLKLQSLSILFLLISCEAKKDKQNDYDIVNGKVIITASMQNY
                     SFGVCAIMSGSIENELSAYNSSDTVKVFYSDIILPPQYEPYILIQDYPPYAGFEILDD
                     STVVRKFSLKIPKSNGPFTNSNRICVTAWCDTSSDARFDWPFEVGRFPYKHFKNGDSV
                     LVIEFIMDENSEYYTLKNDAPVFLKEKNKYDNFIFRF"
     gene            4494..4913
                     /locus_tag="PK937_14075"
     CDS             4494..4913
                     /locus_tag="PK937_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33750.1"
                     /translation="MRIFLITLILLTTGCSTVMRTAYDSSSAQSMSDEDIQSAISTGK
                     ILNVTYVRDSQKSVTLAGRLEKIETGYRFTPRRKGALGAYKRSMDLTRAEIRKIEVIE
                     KEGLCASEAGTFFLIVGLVLIVAASTVVVASPVGPIF"
     gene            complement(5234..5680)
                     /gene="umuD"
                     /locus_tag="PK937_14080"
     CDS             complement(5234..5680)
                     /gene="umuD"
                     /locus_tag="PK937_14080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNH33751.1"
                     /translation="MKKEKSSSAFEIFPADITTARHTPLANDTVSAGFPSPADDFVDK
                     MLDLNEHLIKNKAATFLVRVRGTSMINAGIHDGDILIVDRSLEASNGKIVIGVVNGEF
                     TVKRIQKRGMDLYLMPENDQFQPIRINEDMDFKIWGVVTYIIHKSP"
     gene            complement(5677..6945)
                     /locus_tag="PK937_14085"
     CDS             complement(5677..6945)
                     /locus_tag="PK937_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNH33752.1"
                     /translation="MRNETYTQLMAIQHPWRGIHLLPKPIWVLFWTTLVNRSGMMALP
                     YLVLYLTKELHYTAEDAAAMIVVYGIGAFLTGPVAGKISDRIGAHRIMILSLVATGLV
                     LVTIPLAHEFWAVACLTVLWAVVSEAFRPASLAVVTNLVSVDQRKLAFAVNRLAINIG
                     MSIGPAVGGFIALHSYPMLFYVNGIFSVLASLVMMFSRWPEAHSAHAAAALHREAESE
                     MHPWQNLSFMYFLIPLLLIMLIFFQIDATVALYLVRDMAYPESHFGLLFSVNTVLIIF
                     FEVWINIRMSHWKHRHALSLGALLIAIGFGLMAFSGHIGYIAFTVAVWTFGEMIIFPG
                     SAAYVSDIAPASRRGEYMGYYQMTFSLGLIIGPWLGVLLYEATDAPTLWLTTLAVGIV
                     AAGMLRLVKEPPAVTMKVLSEAKPGGTDNP"
     gene            complement(7001..>7342)
                     /locus_tag="PK937_14090"
     CDS             complement(7001..>7342)
                     /locus_tag="PK937_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33753.1"
                     /translation="IATRRYEPLKHPPLFTLGSIDGLAFYKQSLIAHQSGVVGRIKRI
                     YLDNDLRTATHAAVLEAHNPHFDQPTTGEIGGSFYYYIANAQLRSGFRNKKIKPYHEL
                     RDVVILKIPLE"
BASE COUNT         2257 a         1532 c         1516 g         2037 t
ORIGIN      
        1 ccaattccaa ttccaattcc aattccaatt ccaattccaa ttccaattcc aatggaagcg
       61 gaatagacaa ctaatttata tttgtatatg aaggaggtga gtccgttctt caaattataa
      121 tgttttaagc aatcgtatgt gaagtcaatt aattttaacc ggaacaaaaa aggaagaaca
      181 atattaccga attcagcgct gggtgcattg aatttttaaa gtaataaggt cttcctgttg
      241 tcaacctacg aaatgcaacg tagattttca acttttgttt ccaaaatgag gaattaaaaa
      301 aatgaaacta gcgaagcctc ttttggcatt ggtttatgtc ttgactgtag ctgttttcat
      361 cgcatgttct gaaaacaatg agtcaaacaa gcattcaaac aaatttcaca aaagtcaatt
      421 tctcgaaaaa ggaaatttat ggaaagaatc agagtttgaa tcattactaa gacaaaatcc
      481 tagtaaatct gaagcactaa acgcagttgg tcgttcattg caggtacccg gtgtagggct
      541 tgtttttgat gaagagggtt atctttacac ccctactcaa tttaatcaac tagttgaaga
      601 aattactact tctacaggtg acgatactgg tgccgttata gatcagttga tggatgttta
      661 ttatactgac cttaatacct atgccgatgt tacatctgat ccgaatgccg tagttgaaaa
      721 cactattgga atcgctcctt tggattcaag tgatgagttt acatctccat attatgtttt
      781 ggatatgtat caagaagcat atctacccaa ggaatctaaa ctgaataaac tgaaagcgga
      841 agcatcgtta ttcatgccaa aaggaattga gaaactgagt ggaaccccca atcctcctcc
      901 agggatagtt tttcctgcgg atgttgttat tgctgacaga aaaggctcag catttggcca
      961 tgccggtgtc gtcataattc ccggattcgg taacagcttg caaggcgcga tgatgatgga
     1021 agctgtgggt aatagaaaac ataaagcaga tgaagtagta tcaaggccat cagacaaata
     1081 tttttttcga agcgatttga cctatatgaa aatctgttac cgagaaaata ttaccattca
     1141 acagcgctcg gatattacaa agtatgttgt ggcacaagat cccgatccat attctatatg
     1201 gacttcaaaa gatactggcg gcaaatggta ttgttcaaaa ctgccttgga gagcttataa
     1261 gttaaaagtt ggatgcaata ttgatgagga cggtggattt tgggtcactc catttgatat
     1321 tgcaggagac gcatcgctaa aaggttctta ttattggcat aattaaagta aattacatgg
     1381 tggaccaagg gataaaacta tcccttggtt tcatctttaa ggagtgaaag aatatctatg
     1441 agaataattt ggctgttatt cagtttgtgt tactacggat gcagtactga tcagaaagat
     1501 ttatacttgc atacgggtga caagcttgat tcagagcagt tttataccgg gaacctgact
     1561 tctcaggaat actcagagtg tgctaccatg atcattccgg gaggatttgc aattattaaa
     1621 ccggacacaa ttccaacctt aataaaaaca atccaagtcc cccctatcat ttacagcgct
     1681 caaatggatc aaaacggact tgtttgggta gctcctccct acaatcaaga agacgacaac
     1741 agcgagcgca tatatattat tgacccggtt accgagagag tggttaaatc aatcacgttg
     1801 ccccaggagc tgcgaacgcc tagctgcctt aaaatttctg gaaataaagt atattttcga
     1861 ggcgatcgca atggctttag cgtaggtata ggagctattg atagattgac atataataat
     1921 attgaggcaa ttgccaaatt tgatacaacg ggatttgcaa gtagccctgg tttggaaata
     1981 ataaataaca acatacttgc attttgtgaa accaatggcc gtcagtctag taaaattgtt
     2041 ttattcaatc ccgtaactaa aacaataatt acaaaagtga ttggaaacta ttatcgttat
     2101 acatacgact taaattatat ttatttgact gttcagaccg ctgaaaatca aataatacta
     2161 cagaaaatag acccctctaa cttaaactta ttgactgaag tgttattggg cggtgatgag
     2221 atttatatta cttcaaatca aaataatatt tgtgtcggtt catttgcaaa caaatatatt
     2281 aaaattgtgg agaagtccaa tctaacgatt ttaaaaacaa tagatttttc aaagcaaccc
     2341 ggcatctatt ttaatagaag ctttggtttt ataacgcaac gtatgcttat gctcaatgat
     2401 aattcattct atgatatgca aaacgatata atacatagta aactgtttcc tatcaactat
     2461 cctcagacta tgaatttaac cttagccgag ggtcaaactt taaaataaaa gatcattcaa
     2521 agctgagatc aaaattcgga cttaatctat gatcaaaatc gttctggttt tcatttgttt
     2581 tttctgttct actgcgacaa tatcagccca ggtcaaaacg ccaaacgccg tctatgtaga
     2641 agttataggc aatggcggat tttattcggt taattacgag cggttatttt gtaattattt
     2701 tggattcagg gttggcgctt catatttata cttaggtgat gtcggccaat acgcatcatt
     2761 tcctattttg gcaaattttt ttatgggtgg caaacatcac aaatttgaat tgggggttgg
     2821 atgtaccgtg atgaacgacg cgccggtggg agaattttcc aattgggata cgaaatattt
     2881 cagaacaatc actttaggtt atcgttacca gtcgaaagaa aatttttttc ggatcggatt
     2941 tacgccgcta tttaccggtc atgagatcat acctttgggc ggcatcagtt tcggtcgcgc
     3001 tttttgattt atttttttct gattaatgat agttctcgtg cacgtgaatt cagcgataga
     3061 agatagtaga gcaagttttg actatgcgat aacaaggaaa aactatgtag ccatcaaccg
     3121 cttattagtt tggccattcc attaacaaat taaccttaat caaggcgatc aaaatattgg
     3181 tcgccttgtt aatataaatc aaagctttca aaatgaacat catattcaaa actgtattta
     3241 taatcctaat cgcttcaatc cacgtttccg ctcaagatca ttgtttgcca tcacaatggt
     3301 tcgtaggcct tgacataggc ggcggcggcg tttattggaa cttggatgat ccatccgaac
     3361 cgggaagttt taacggagcg ggcggcatat tcaacattcg tacttcgatt aattacaaaa
     3421 tgaatcgttt cagcataggg cttggcggaa agtatgataa aatctatgtt ggcgaacttc
     3481 acaacacgat taatcacgat atagtcaaga aatttgacag aggttacatt gcgcaaacca
     3541 agtttttttt catcgttgag tatgatttat ttaaaagaaa gaattggagc ataggatcgg
     3601 ctatctcagc cggtcttgcg cacactaaag ccggtctcga tggatcgtta agcaaaaaca
     3661 acccttattt cggtatcagc acatatctga aatggaacga tcaccataaa ttaacttata
     3721 gcattcgccc atacgcggaa attcaatcta taaaaaatca taccacttca ttgcaacaac
     3781 atacggtcgt agatttcgga tgcgcgatag gcttgcagtt tgtagttttt taaagggata
     3841 gaaaaaaact ttttatgcga ctcatgcctt cattaaaact tcaatcattg tctatcttat
     3901 tccttctgat ttcctgtgaa gccaaaaagg ataaacaaaa cgattacgat attgttaatg
     3961 gaaaagtaat cataactgca tccatgcaga actactcttt tggggtttgt gctattatgt
     4021 ccggcagtat tgaaaatgaa ctttcagcat ataattcatc cgataccgtt aaagtatttt
     4081 attccgacat tatactgccg ccacagtacg agccatacat tcttatccaa gattatccgc
     4141 cttatgcagg ttttgaaatt ctggatgata gtacagtagt acgaaaattc agtttgaaga
     4201 tacccaagag taatggccca tttacgaata gcaaccgaat atgcgttacg gcgtggtgcg
     4261 acacgtcgag tgacgcgcgc ttcgattggc cgtttgaagt tggacgattt ccatataagc
     4321 atttcaaaaa cggtgatagt gttttggtta tagaatttat catggatgaa aactcagagt
     4381 actatacatt gaaaaacgac gcgccggttt ttttaaaaga aaagaataag tacgataatt
     4441 ttatttttag attttagaaa ttaaagaact atttcatcgc aaggaaaccg tcaatgagaa
     4501 ttttccttat aacattaatt cttctcacca ccggatgcag tacagtgatg cgcacggcgt
     4561 atgattcttc atcggcgcag agtatgagcg atgaagatat tcaatccgcc atatcaacag
     4621 gaaaaatact gaacgtcacg tatgtacggg attcacaaaa aagtgtaact ctggccggaa
     4681 gattggaaaa aattgaaacg ggttaccgct ttacgccccg ccgcaaagga gcgctgggcg
     4741 cctacaagcg gagtatggac ctgacccgcg ccgagatacg taaaatcgaa gtgatcgaaa
     4801 aggaaggcct gtgcgcatca gaagccggta cgttttttct aatcgtagga ctcgtattga
     4861 ttgtagcagc ttccactgtc gtcgtagcat ctcccgtagg ccccattttt taaatcacaa
     4921 gtaagatacc taagggatac tataccttcc gcctacgacg aaattgctcg actaaacatc
     4981 acaaataaaa attatttaga tagaactttg cgcacttagc gtcttcgtgc gtacgcattc
     5041 cctctcgcaa agccgcaatg aaaaaccaag tagatgcgtt gattttacat tgcctcaccc
     5101 gcgcaagcgg gtgtctataa aattatgaga ttctttataa gactcacgaa taaaaattat
     5161 ttagatagaa ctttgcgcac ttagcgtctt cgtgcgtacg cattccctct tgcaaagccg
     5221 caaaaaaacc cagtcaaggc gacttgtgga tgatatacgt tacgacaccc caaattttga
     5281 aatccatatc ttcgttaatg cggatgggtt gaaattgatc gttctccggc atgagatata
     5341 aatccatgcc gcgtttctga atacgcttta cggtgaattc cccgttgact acgccgataa
     5401 cgatttttcc gttggacgct tccagcgaac ggtccacgat cagaatgtcg ccgtcatgaa
     5461 taccggcatt gatcatcgac gtgccgcgca cgcgcacgag aaacgtagcc gctttgtttt
     5521 tgatcaaatg ctcattgaga tccagcatct tatctacgaa gtcatccgcc ggcgaaggaa
     5581 accccgccga aaccgtatcg ttggctagcg gcgtatgacg cgcggtcgtg atgtccgcag
     5641 gaaagatttc aaacgccgaa gatgattttt cttttttcat gggttatccg taccgcccgg
     5701 ttttgcctcc gataagactt tcatggttac cgccggcggc tctttgacca aacgcagcat
     5761 accggccgct acgatgccga cagccaatgt cgtcaaccat aatgtcggcg cgtcggtcgc
     5821 ttcatacaaa agcacaccca accacggccc gatgatcaat ccgagactaa acgtcatttg
     5881 atagtagccc atatattctc cgcgccgcga tgccggtgca atatccgata catacgccgc
     5941 cgagccggga aaaataatca tttccccgaa tgtccacacc gccacggtaa aagcaatgta
     6001 gccgatatgc ccgctgaaag ccatcaatcc gaaacctatc gcaatcagaa gcgctccgag
     6061 actgagcgca tgccggtgtt tccagtgact catacgtata ttgatccaga cttcgaaaaa
     6121 aatgatgagt accgtgttca ccgaaaataa taacccgaaa tgggattccg gatacgccat
     6181 atcgcgaacg agatacagcg ccaccgtcgc atcaatctga aaaaagatca gcatgatcaa
     6241 taatagcgga atcaaaaaat acatgaatga aagattctgc cacgggtgca tttccgattc
     6301 ggcttcacga tgaagcgcgg ccgcggcgtg cgccgaatgc gcttccggcc aacgggaaaa
     6361 catcatcacc agactggcta acaccgaaaa tataccgttc acataaaaca acatcggata
     6421 cgaatggagt gcaataaatc cgccgacggc ggggccgata ctcatgccga tattgatcgc
     6481 aaggcgattg acggcaaaag ctagtttgcg ttggtcaacg gaaaccaaat tggtcacgac
     6541 cgcaaggctc gccggtctga aagcttcact tacgacggcc catagtacgg tgagacaagc
     6601 cacagcccaa aattcatgcg ccaacggaat agtcaccaaa accaaacccg tcgcaaccaa
     6661 agataaaatc atgatgcgat gtgcaccgat gcgatcggaa attttaccgg ctaccggccc
     6721 ggttaaaaac gctccgatac catacacgac gatcatcgcc gcggcgtcct cagcggtata
     6781 gtgcaattct ttggtaagat aaagaacaag gtacggcaat gccatcatgc cgctgcgatt
     6841 gactaaggtc gtccaaaaaa gtacccaaat cggtttcggc agaagatgta tcccgcgcca
     6901 gggatgctga atagccataa gttgtgtata tgtttcgttt cgcaaacata cacagcgtag
     6961 catgaatttc aaaggacgat ttttacgaca aacgcgtgat ttactccaaa gggattttca
     7021 gaattacaac gtctcggagt tcgtgataag gtttgatttt tttattccta aacccgctcc
     7081 gtaattgcgc attggcgatg taataataaa atgaaccgcc aatctcgccc gtcgtcggtt
     7141 gatcaaaatg cggattgtgc gcttccaaaa ccgctgcgtg tgtggccgtg cgcagatcgt
     7201 tatccagata aattcgtttg atccgtccaa cgacacccga ctgatgcgcg atgagtgatt
     7261 gcttgtaaaa agccagcccg tcaatgcttc cgagcgtaaa taacggcgga tgcttcaaag
     7321 gttcataccg gcgtgtagca at
//
LOCUS       DAOQGF010000143         7339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4937_length_7339_cov_5.567271, whole genome shotgun sequence.
ACCESSION   DAOQGF010000143 DAOQGF010000000
VERSION     DAOQGF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_7339_cov_5.567271"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PK937_14095"
     CDS             <1..745
                     /locus_tag="PK937_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH33754.1"
                     /translation="GTRTFAENKGDHYVINGSKIFITSAGYADTFVITAVTDKTKGKS
                     GISSFIVEKTFPGFIIGKKEDKMGWRASDTRMLHFENMIVPKENLLGKPGEGFKGFLK
                     VLDGGRVGVAALSLGIAIGAYEEALQYSKTRMQFERPISDNQGIQFMLADMALGIQCS
                     WHLMLHAARLKDAGQSFTKEAAMSKLHATELAMQNCIKAIQILGAVGCTDRYNSERFF
                     RDAKIGEIGEGTSEIQRLIIAREILKTIA"
     gene            751..3159
                     /locus_tag="PK937_14100"
     CDS             751..3159
                     /locus_tag="PK937_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNH33755.1"
                     /translation="MPDLLNRNRIARAILIKSPALFMRVVFGKKHLIAKIGIILALII
                     GPVLMFPDSDFTRYSEYKIGSISPDEVRAPFTFPVYKTPDQLTTERRDAQSLVAPVFD
                     RNSALAKSQLANLDEFINYLDLLRNAPKPFEYKDNDGQTQNYIPPAFDSVKSAIIRKF
                     GFSVMEERWAFLVNKENATIDNNYGQMRSDTLRLLKRATIGRSLTRSQFLELSRDWMR
                     ILTDLFALGIIDEDKNNFKSPISPINVREGLKESTEFIKNVNDVDEARYHTQDLLKTY
                     YSDTKLINLSYEVLSRFIIPNILANRDETEARKREASANVPQTYGFVLSGDIIVNKNE
                     TITSKTHQQLQSLEATWAQRREAEGGVKWLLPFIGRSFLVFSLMFFLLGYIYIYRPDI
                     FSSIRKLSLLVSLILLEITFYYVFIHVLKFPAFVIPIVFSSVLLTTLFDVRIGLIGTI
                     SISFLIGAMQGNEYTTTIVLLFVGSVACITVVNFSRRSHIFSSVLWVSGAYIFIIATT
                     SFVKYTEFAEIFLHQLPYAIVSGMSSMLVAFGCLVLFEQVFDVSTTFTLLELSDSNHP
                     LQQQLALKAPGTYHHSVIVANLSHAGAEATGANSLLARVGALYHDIGKIEMPEYFIEN
                     QIGGVNKHDRLDPHMSAKILLSHIHSGLELAEKYRLPKIIRSYIPEHHGNLLMTFFYH
                     RAMENKKPDAVISEASFRYPGPRPKTKESGIIMLADGVEAATHAIKEPNAENIRNVVN
                     EIIERRLKSGELDECELTIGDLKRITEAFIPIILGIYHVRVEYPKADPLPIDGKNVTV
                     GAMS"
     gene            3156..3581
                     /gene="ybeY"
                     /locus_tag="PK937_14105"
     CDS             3156..3581
                     /gene="ybeY"
                     /locus_tag="PK937_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNH33756.1"
                     /translation="MITITNTHKHRRVPKQRIIALTKMILKKESPRNLEQFHFVFLDN
                     VTMRRFNKKFLNHDYPTDTITFDLSANRRASLEGECYISLDQAVMQAKEYGVTYTNEV
                     LRLCAHCVLHLLGYHDDTDQQRSRMIRRGDHYLTLLERQ"
     gene            3578..4432
                     /gene="nadC"
                     /locus_tag="PK937_14110"
     CDS             3578..4432
                     /gene="nadC"
                     /locus_tag="PK937_14110"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNH33757.1"
                     /translation="MNQHLTIHPVLRTQIKHAIEEDLGFGDVTTLSTVPAEQIGRGYV
                     RAKSEGVLAGQLVFNEVFRQINPQIKLEWSAKDGDTLVKGQICLHFAGSMRSILIAER
                     TALNFIQRMSGIATLTRHFMERIKHTSAQILDTRKTTPLWRELEKYAVRCGGGSNHRI
                     GLYDMFLIKDNHITAAGGIGAAIEKAIEYNRQNNLHLSIEVETKNLDEVREALRYPVQ
                     RIMLDNMKIETMREAVAIINKRCETEASGNVSLDTVKDIAETGINFISIGALTHSAPA
                     LDLSLLVE"
     gene            4624..5088
                     /gene="rpiB"
                     /locus_tag="PK937_14115"
     CDS             4624..5088
                     /gene="rpiB"
                     /locus_tag="PK937_14115"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNH33758.1"
                     /translation="MLKQGAERKVALGADHGGFPLKEFLKKVLIEMQFTIVDVGTHST
                     ESVDYPDFAEAVAKQVATGECFRGIMIDGAGIGSCMVANKVSGVRAAMCYDITTAVNS
                     REHNNANVLTLGSKMIGETVAEHIVKVFLKTDFAGGRHQGRIDKMMAVEKKR"
     gene            complement(5384..6508)
                     /gene="ribB"
                     /locus_tag="PK937_14120"
     CDS             complement(5384..6508)
                     /gene="ribB"
                     /locus_tag="PK937_14120"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNH33759.1"
                     /translation="MHFNTIEEAVEDYKKGKIVIVVDDEDRENEGDFIMAAEKVTPDA
                     VNFMAKHGRGLICLSITRQRASELELNIMVDRNTSLHTTPFTVSIDAKEGTTTGISAA
                     DRAKTISVVIDPVTRPEDLARPGHVFPLVARDGGVLERSGHTEATVDLARLAGLYPAG
                     ILCEIMDDDGSMARVPRLMEMAKTFDLKIITIKDLIEYRRRMERFIRPVVKDVELPSR
                     YGNFSVTVYENILNGDQHIAITKGLIKGEDAILVRVQVENTMGDVFGSLTGNNSSHVS
                     DSLRMIESAGRGVVLYLRENDQRSLGLFPSEEVSSLDSHHSRDTDKDWRTLGVGSQIL
                     VDLGVRRLKLLTNNRKKYVGLEGYGLEIVEQIPIVSQAKV"
     gene            complement(6511..7149)
                     /locus_tag="PK937_14125"
     CDS             complement(6511..7149)
                     /locus_tag="PK937_14125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNH33760.1"
                     /translation="MFTGLIEETGKVASVIPVGQGRRFTIRASKVMDGLSIDDSVAVN
                     GCCLTVIARTSDSFDVEAVEQTLSKTTLGEFEIGTVVNLERAMQLNDRLGGHLVLGHV
                     DGVGSIVTIEERSLSHWVTIAIPAELEKYVIAVGSITLDGISLTVANLKENCISVAII
                     PHTWKVTNFAKRSPGDRINVEVDMIGKYVEKLMTRKPEEQKITAEWLLDQGK"
     gene            complement(7153..>7339)
                     /locus_tag="PK937_14130"
     CDS             complement(7153..>7339)
                     /locus_tag="PK937_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNH33761.1"
                     /translation="GGRSASYEEELRKAKGIAIQEMEAQAAALGANAVIGVDLDYETV
                     GQSMLMVTAAGTAVYIE"
BASE COUNT         1976 a         1801 c         1600 g         1962 t
ORIGIN      
        1 cggtacacgc acgtttgccg aaaataaagg cgatcattat gtgatcaacg gctctaaaat
       61 ttttatcact tcggcgggtt acgcggacac ttttgttatc accgccgtta cggataaaac
      121 caaaggtaaa agcgggatca gttcgtttat tgttgaaaaa acgtttcccg gttttatcat
      181 cgggaaaaaa gaagacaaaa tgggttggcg cgcatcggac acgcgtatgt tgcactttga
      241 aaatatgatc gtgcccaaag aaaatcttct gggcaaaccc ggcgaaggat tcaaaggttt
      301 tctcaaagtt cttgacggcg gtcgcgtcgg cgtcgccgcg ctctcactgg gtatcgcgat
      361 cggtgcgtat gaagaagcgt tgcaatattc taaaacacgt atgcagtttg aacgtccgat
      421 ttcggataat cagggcatac aatttatgct ggcagacatg gcgctgggta tccagtgcag
      481 ctggcatttg atgcttcatg ccgctcgcct caaagatgcc ggccagtctt ttaccaaaga
      541 agcggctatg tccaaactgc atgcgacgga attggccatg caaaattgca tcaaagccat
      601 tcagattctc ggcgccgtcg gatgcaccga ccgctataat tcggaacgtt ttttccgtga
      661 tgctaaaatc ggcgaaatcg gcgaaggtac ttctgaaata caacgcctca tcattgcgcg
      721 tgaaatatta aaaacgatag cttaaatctc atgcccgatt tactcaaccg aaatcgcatt
      781 gcgcgcgcta ttctgatcaa atcccccgct ctctttatgc gcgtggtttt cggtaaaaaa
      841 catcttatag ctaaaatcgg tattatcctt gctctgatca tcggccctgt gttgatgttt
      901 ccggacagtg attttacccg ttactccgaa tacaaaatcg gctcgatttc acccgatgaa
      961 gttcgcgcac catttacgtt tcccgtatac aaaacgccgg atcaactgac gacggaacgc
     1021 cgtgatgctc aaagtttagt cgcaccggta tttgatcgca attcggcact cgctaaatcc
     1081 caattagcca accttgatga attcataaac tacttggacc ttttgcgtaa tgcgccgaaa
     1141 ccttttgaat acaaagataa cgacggccag acacaaaact atatccctcc cgcatttgat
     1201 tccgtcaaaa gcgcgattat ccgaaaattc ggctttagtg ttatggagga acgttgggct
     1261 tttttggtca acaaagaaaa tgcaacgatt gataataatt acgggcaaat gcgttccgac
     1321 acccttcgtt tgctcaaacg tgctaccata ggccgctcgc taacccggtc gcaatttctg
     1381 gaactgtctc gcgactggat gcgcatactc accgatcttt ttgcgctggg cattattgac
     1441 gaagataaaa ataatttcaa aagcccgatc tccccgatca acgtacgcga aggtcttaag
     1501 gaaagcacgg aatttatcaa aaacgtcaac gatgtggatg aagcacgcta ccacacgcaa
     1561 gacttgctga aaacctatta ttcggatacc aagctgatca atctgagtta tgaagtgctc
     1621 tcccgtttca tcattcccaa tattttggca aatcgcgacg agacggaagc gcgtaaacgt
     1681 gaagcttcgg ctaatgtccc acaaacctat ggcttcgttt taagcggcga catcatcgtt
     1741 aataaaaacg aaaccatcac ctccaaaacg caccagcagt tgcaatcact ggaagccacg
     1801 tgggcgcagc gccgcgaggc ggaaggaggc gttaaatggc tattgccgtt tatcggacga
     1861 tcgtttttag ttttttcact gatgtttttt ttactgggtt atatttacat atacaggccc
     1921 gatatttttt cgagtattcg aaaattatca ctgctcgtgt ccttgatttt gctggagatc
     1981 actttctatt acgtgttcat tcacgtgctc aaatttccgg cctttgtgat tcccatcgta
     2041 tttagttccg tcctgctgac aacacttttt gacgtacgta tcgggctgat cgggacgatc
     2101 tcgatcagtt ttttgatcgg agcgatgcaa ggcaatgaat acacgacgac cattgtattg
     2161 ttatttgtcg gttcggttgc gtgcattacc gtggttaatt tcagtcggcg cagtcacatt
     2221 ttcagttccg ttctctgggt gtcgggcgcg tacattttta tcattgcgac gaccagcttt
     2281 gtgaagtata ccgaatttgc tgaaattttt ttgcatcagt taccttacgc catcgtcagc
     2341 ggcatgtcgt ccatgttggt cgcattcgga tgccttgtac tttttgaaca ggtttttgat
     2401 gtcagtacga catttacttt actcgaactt tctgattcca atcacccttt gcaacaacaa
     2461 ctggcgctga aagcgccggg cacttatcat cacagtgtaa tcgtcgcaaa tctgtcccat
     2521 gccggcgcgg aagcaaccgg cgcgaattcg ttgctggcac gcgtcggcgc cttgtatcat
     2581 gacatcggta aaatcgagat gcccgaatat ttcatagaaa atcagatcgg cggtgtgaat
     2641 aaacacgatc ggctggatcc gcacatgagc gcaaaaatac tgctctcgca tattcattcc
     2701 ggcctggagt tagccgaaaa ataccgtttg ccgaaaatca tccgaagtta tatcccggaa
     2761 catcacggta atctgctcat gacctttttt tatcatcgcg cgatggaaaa caaaaaaccg
     2821 gatgccgtga tatccgaagc ttcatttcgt tatccgggcc cgcgccccaa aaccaaagaa
     2881 tccggcatta tcatgttagc cgacggcgtc gaagccgcaa cccatgcaat caaagaaccc
     2941 aatgctgaaa atattcgcaa tgtggtcaat gaaatcatcg aacgccgtct caagtccggt
     3001 gaacttgacg aatgcgaact gaccatcggt gacctcaaac gcattaccga agcttttatc
     3061 ccgatcatat tgggcatcta ccacgtgcgt gtcgagtatc ctaaagccga tccgttgccg
     3121 attgacggga aaaatgttac cgtaggcgcg atgtcatgat cacaataacc aatacccaca
     3181 aacatcggcg cgtaccgaaa caacgtatta tcgctttgac gaaaatgatc ctgaaaaagg
     3241 aatcgccaag gaatttggaa caatttcatt tcgttttttt agataatgtt acaatgcgac
     3301 gtttcaataa aaaatttttg aatcacgatt accccaccga tacgattact tttgatctca
     3361 gtgcaaaccg tcgcgcctcg ctggaaggcg aatgctatat cagtttagac caggccgtga
     3421 tgcaagctaa agagtacggc gtcacttata ccaatgaagt tttacggctt tgcgcgcact
     3481 gtgtcctgca cctcttgggc tatcatgacg ataccgacca acagcgttcc cgtatgatcc
     3541 ggcgcggcga ccactatctt actttactgg aaagacaatg aatcaacatc ttacgataca
     3601 ccctgtttta cgtacgcaaa tcaaacacgc tatcgaagaa gatctcggct ttggcgatgt
     3661 aacgacactc tcaaccgtgc cggccgaaca aatcggacgc ggttatgtac gtgctaagtc
     3721 ggagggcgta ttagccgggc aacttgtttt caatgaagtc tttcgccaaa tcaatcctca
     3781 aatcaaattg gaatggtcgg ccaaagacgg tgatacgctt gtaaaaggtc aaatctgcct
     3841 gcattttgcc ggcagcatgc ggtctatcct cattgcggaa cgtacggcgc ttaattttat
     3901 ccagcgaatg agcggtatcg ccacattgac acggcatttt atggagagaa tcaaacacac
     3961 atcggcgcaa attttagata cgcgtaaaac tacaccgctt tggcgggaac tcgaaaaata
     4021 cgccgtacga tgcggcggag gttccaatca tcgcatcgga ttgtacgata tgtttttgat
     4081 caaagataat catatcacgg cggccggagg tatcggcgca gccatagaaa aagccattga
     4141 atacaaccgc caaaataact tacatttgtc catcgaagtt gaaaccaaaa acttagatga
     4201 agtgcgcgaa gcattgcgtt acccggtcca gcgcatcatg cttgacaata tgaagatcga
     4261 aaccatgcgt gaagctgttg caataatcaa taaaagatgt gaaaccgaag catccggtaa
     4321 tgtttccctt gatacggtaa aagatatcgc cgaaaccggc attaatttta tttccatagg
     4381 cgcgctgaca cactcggcgc cggcgcttga tctttcactt ttagtagaat aatcattacg
     4441 atgaaacgaa aattaataac tgaacgtaca gtcatcgaaa ccgtcaaaag cggacagaaa
     4501 acgatcatga tctcctcgga cgctttgatc acggcattag cacgtgataa agccaaacag
     4561 ttaggtgcga catttcaaat cgttaagccg caaaccgttg ccccatctac agtttccaat
     4621 ctactgctca aacaaggcgc agagcgtaaa gtagccttgg gcgcggatca cggtggattt
     4681 ccgttaaaag aattccttaa aaaagtactt attgaaatgc agttcaccat cgttgatgtt
     4741 ggaacccatt cgacggaatc ggtagattat ccggattttg cagaagccgt ggcaaaacaa
     4801 gtcgcaaccg gtgagtgttt tcgcggtatt atgattgacg gtgcaggtat cggttcatgt
     4861 atggtagcca ataaagtatc cggcgtacgg gcagcgatgt gttacgatat cacgacagcc
     4921 gtcaacagcc gtgaacataa taacgccaat gtcttaacgc tcggctctaa aatgatcggc
     4981 gaaaccgtcg ctgaacatat tgttaaagtt tttctcaaaa cggattttgc cgggggaagg
     5041 caccagggac gtattgataa aatgatggcc gttgaaaaga agcggtaagc tgctgtagat
     5101 ataaaaacca aatcgtttta tttttccctt ccgatagaat ttattaaatc cttttgaaat
     5161 gcattacttc atctcgccct gccctactgt gctattgacc gtcgaatacg tgacgctgtt
     5221 ttcactacac tctttcttac aagaacttct cagaaaatac gtaaattata tcaagatttt
     5281 catttggtaa gacatgtaga agattagcta agctgtatcc tatcgggagt tacgggaaat
     5341 ctgagttact accgcgagag aaacatattc aaaaaagatt ggtttaaact ttggcttgcg
     5401 atacgatcgg aatctgctcc acgatttcca acccataacc ttcaagtccg acgtattttt
     5461 ttcggttgtt ggttaaaagt ttaaggcggc gcacccccag atcaaccaat atttgagatc
     5521 cgacacccaa ggtacgccaa tctttatccg tatcacgcga atgatgactg tctaaagatg
     5581 atacctcttc ggatggaaaa agtccgagcg agcgctgatc attctcacgc agatacagca
     5641 cgacgccccg tccggctgat tcgatcatgc gtaagctgtc ggatacatgc gatgaattat
     5701 tcccggtcaa tgaaccaaac acgtcaccca tcgtattttc aacctgcaca cgaactagaa
     5761 tcgcatcctc gcctttgatc aaacctttgg ttattgcaat gtgctgatcg ccgttgagta
     5821 tgttttcata caccgtaacg gagaaattcc cgtaacggct cggtaattcg acatctttga
     5881 ctacggggcg aatgaagcgt tccatccggc gacggtattc gatcaggtct ttaatggtaa
     5941 ttattttgag atcaaaagtt ttagccattt ccatcagcct cggtacacgc gccatcgaac
     6001 cgtcatcatc catgatttcg catagtattc ctgccggata aagtcccgca aggcgtgcaa
     6061 gatctacggt cgcttcggta tgaccggagc gttccaatac gccgccgtca cgtgccacca
     6121 aaggaaaaac atggccggga cgtgcaaggt cttccggtcg cgtcaccgga tctatcacta
     6181 cggaaatagt ttttgcacga tctgcagccg atatcccggt tgtcgtgcct tcttttgcat
     6241 caatgctcac ggtaaacgga gtcgtgtgca atgaggtgtt gcgatccacc atgatattga
     6301 gctcaagttc tgaggcgcgt tgacgcgtta tggacaaaca tatcaaacca cggccgtgtt
     6361 tcgccataaa attaaccgca tccggagtga ccttttcggc ggccatgata aaatcgcctt
     6421 cattttcgcg gtcttcatca tctactacga tgacgatttt accttttttg taatcttcta
     6481 cggcttcttc gatggtgtta aaatgcatat ctattttccc tgatccagga gccattcggc
     6541 tgtgattttc tgttcttccg gttttcgtgt cattaatttc tccacgtatt ttccgatcat
     6601 atccacttct acattgattc ggtcgccggg agatcgtttg gcgaaattgg ttactttcca
     6661 agtatgcggt atgatcgcca cagatatgca attctccttc aaattcgcca ccgtaaggct
     6721 gatgccatcc agcgtgatcg aacctaccgc aataacatac ttttccaatt cggccggtat
     6781 ggctatcgtt acccagtgac tcaaagaacg ctcttcgatg gtcacgatag aacctacacc
     6841 gtccacgtgt ccaagtacca aatggccgcc caaacgatcg ttgagttgca tcgcacgttc
     6901 cagattaact accgtaccga tttcaaattc gccaagtgtg gttttggata acgtctgttc
     6961 taccgcctcc acatcaaacg aatcgctcgt tcgcgcgatc accgtgaggc aacatccatt
     7021 gacagcgaca ctgtcatcta tggataatcc atccatgact ttagatgcgc ggatcgtaaa
     7081 acgacgccct tgccccaccg gaatgacgga ggctacttta cccgtttctt caataagtcc
     7141 tgtgaacata aattattcga tgtaaacagc cgtgcccgcc gcagtgacca tcagcatact
     7201 ttgaccgacg gtttcataat ccagatctac tccgatcacg gcattcgcac ccaacgccgc
     7261 ggcttgcgct tccatttctt gtattgcaat acctttggct ttgcgcaatt cttcttcata
     7321 cgatgccgaa cgtccgccg
//
LOCUS       DAOQGF010000144         7325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_4949_length_7325_cov_6.459422, whole genome shotgun sequence.
ACCESSION   DAOQGF010000144 DAOQGF010000000
VERSION     DAOQGF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_7325_cov_6.459422"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..189)
                     /locus_tag="PK937_14135"
                     /pseudo
     CDS             complement(1..189)
                     /locus_tag="PK937_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
     gene            192..518
                     /locus_tag="PK937_14140"
     CDS             192..518
                     /locus_tag="PK937_14140"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNH33762.1"
                     /translation="MIYFYRVKNRKVDKIQAQRKRIDRQLVNDPFLTPEQKSILRSRF
                     SNLTSDLDGALTDRNLYGWLFAISHLMGMIDAYVDAHLFNFDEKMELAYGTDGRYFNV
                     AMRITF"
     gene            552..1460
                     /locus_tag="PK937_14145"
     CDS             552..1460
                     /locus_tag="PK937_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNH33763.1"
                     /translation="MPAASDAKKRREPKNAERIQKILMNSDKMASLGQLIAGVAHELN
                     NPISFIESNVIFLEKYCNDLRTVITMLESSQTMDADVRAKVEAYKKEMEWDFAMGDME
                     NILKSFNEGATRIRSILASLSAFSRGNRIAREEMNIHEAIDNTINLLRYKSRHVVEFE
                     TKYKANPYIIANRGEINQVLMNLIINAIHAVQHKFENGGKGAIYIHTHSGENFVYIDI
                     KDNGEGIPDIYKNEIFKAFFTTKKEGEGTGLGLPISKEIVEKHGGAIEFESRAGIGTC
                     FTVKLPFGTDEETDVPKDTHNQGKKS"
     gene            1457..2677
                     /locus_tag="PK937_14150"
     CDS             1457..2677
                     /locus_tag="PK937_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNH33764.1"
                     /translation="MSVATVASPSMTEPNKIMHDILVVDDEVDNLDLLKRTFRREYNV
                     YSANSAAEALKLLDEREFAVIVSDQRMPEMTGVEMFQKAREKYPQTIRILLTGYTDIN
                     ALVDAINMGHVYRYVTKPWSREEIVMTVKRAVEHYETTKQNARLLDELKIKNEELDRN
                     NKQLKRLDELKTRFMVISSHELRTPASIISGNLELLMSGAMGEVPPEHQEIVTNAYKG
                     TTRLIDLIEDVLSVMRSDSHNLKLNLSAYSPREVLEEVISSYRAFLHERRQTVQNELP
                     ELTIEGDRDRMFHVFANALSNAMKYSRDGETIGVSGKLNGDHMRITFRDAGIGIPPEE
                     LERIFEKFYQLGDADQHRTSKHKFMGGGSGLGLTIVRGIVEEHHGRVWAESEGKDKGA
                     ALQIELPLQQPVAG"
     gene            2693..3667
                     /locus_tag="PK937_14155"
     CDS             2693..3667
                     /locus_tag="PK937_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH33765.1"
                     /translation="MPQSLSYVHSVHLALTRRCGAQCNYCTFKQSDSPLMTFDEIEQV
                     IRKQKPTGISTVVLGAGQSLERLANLPALWNDQGYASFIHYVQDVCRIVLEHQLLPVV
                     DIGPLTYAQLEMLRPFVPTIHLALENINTDFRATVQQNKSADDIMETFSDAGILGIPI
                     TTGLLFGAGESIDDGLATLNALDEIQKRYHNLQSVTLQYVYDTKRGNATRTDIEYLDM
                     LVKYSRKVMPDVAVIVPIHAQLHWLDEGLLPDDIGFVFEGFDGINYDAPFPKLTELER
                     QFAKNHITLVPRLPVFPDFIDRIDASESLRSVLADWQSKRMYVTYAKA"
     gene            3674..4309
                     /gene="upp"
                     /locus_tag="PK937_14160"
     CDS             3674..4309
                     /gene="upp"
                     /locus_tag="PK937_14160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNH33766.1"
                     /translation="MTSFANLHVIRHPLVQQKLTYLRDKNTSHREFRKLLSEITAFMI
                     FEITQDLKTKDIQVETPLETTTAQVLADHVVLVPVLRAGLGMLDGALDLIPNAKVGHI
                     GLYRNEQTLEPVEYYAKFPPTLPEATAIMLDPMLATGGSSSAAVALLKRKKARHIKCL
                     SLVAAPEGVERMLKDHPDVMIYTAGLDRQLNEHGYIVPGLGDAGDRLYGTK"
     assembly_gap    4372..4471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4606..5238
                     /locus_tag="PK937_14165"
     CDS             4606..5238
                     /locus_tag="PK937_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33767.1"
                     /translation="MKHIFFGLCFFALNLSLVAQEEFDETAFESGDAKLGNGWGMNVQ
                     VSTVGFVIGGVYNYKVAQHTWLTSSLDLFWVNGENEQQVYDYNTGQTISINSETILML
                     PLVFQMKRRLFPETMSSNLRPFVVGGGGVVYGWFIDSDVSKSRLPKPQENTQYTYTLI
                     GGIGTDIGKPGSTGYGLDVKYQWLRYSNYLGARKKFDNIQVGFHVNFRSN"
     gene            5244..6386
                     /gene="mnmA"
                     /locus_tag="PK937_14170"
     CDS             5244..6386
                     /gene="mnmA"
                     /locus_tag="PK937_14170"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNH33768.1"
                     /translation="MEIQIKQKKRVVVAMSGGVDSTASAALLKEQGYEVIGVTMKLWN
                     YDEVGGGEINRESGCCSIDTMHDARVVCEQLGVPHYVWDLSDEFGRAVIDNFVNEYLE
                     GRTPNPCVMCNKHIKWGTFLTKALHLGADFVATGHYARSVYDPVSGKYTLRKSYNRKK
                     DQSYALWGIRQKALAMTLFPVGEMSDKEEVREVASRLHLRTAKKKESQEICFITDNNY
                     NRFLKEKISGLSEKLAHGMIKTHDGQVVGEHDGYAFYTIGQRRGVGVAMNEPVYVTEI
                     SAKDNTIYVGRKDDLLATGLTATQVNLCRHEAITEAMPITAKIRYNDVGHEGVLTLNE
                     IGETQVIFNQPQRAITPGQSVVFYDGDEVIGGGIIDRVLKETLQPV"
     gene            6393..7211
                     /gene="panB"
                     /locus_tag="PK937_14175"
     CDS             6393..7211
                     /gene="panB"
                     /locus_tag="PK937_14175"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNH33769.1"
                     /translation="MSTHHTITKVTTEQLRRMKQSGEKISALTAYDAIMASLLDESGM
                     DMILVGDSASTVFAGNDTTLPMTMEQMLYHVRVVTKSVKRALVVADMPFMSYHVSVEE
                     AIRNAGRFMQEGHAEAIKLEGGIEFLPTIQRLVEIGIPVMGHLGLTPQSIHKFGTYRT
                     RGKQPDEAKKIISDAKALEKAGVFALVLEKVPVTLAKKISASITIPTIGIGAGPHCDG
                     QILVTHDMLGLYEKFHPRFVRRYAELGKAMRKAFASYHQDVKSNRFPTDKESYS"
BASE COUNT         1998 a         1585 c         1808 g         1834 t
ORIGIN      
        1 ctcgccgtcc tggctgcgct tgtcctccag gtactcgcgc agcatcttga ccgatccgcg
       61 cgtcagctcc tggctgtcgt cctccagcca tacgcgcact tcctcggggt tcttcttgta
      121 cgccgtggca agctcgttga taacggtcac gtccttgctg cgaccagcgt tgaacgcctt
      181 ggcaatcggc gatgatctat ttttaccgtg ttaaaaaccg gaaagtagat aagatacaag
      241 cccaacgaaa acggatagac cgtcaattgg ttaacgatcc ttttctcacg ccggaacaga
      301 agtccatttt gcgatcaaga tttagcaatc tgacaagcga tttggatggt gcattgacgg
      361 atcgcaattt atacggatgg ttatttgcca tttcgcattt gatgggaatg attgatgcgt
      421 atgtggatgc gcacttgttt aattttgatg aaaaaatgga acttgcttac gggacggatg
      481 gccggtattt taacgtggct atgcgtataa cattttaacg ggacattgtg aaggcccaaa
      541 agaaaaaaat aatgcctgcc gcttccgacg ccaaaaaacg ccgggaaccc aagaatgccg
      601 aacgtattca gaagattttg atgaattcgg ataaaatggc atcactgggt caactgatag
      661 cgggcgtagc gcacgaactc aacaatccga tcagctttat tgagagcaat gttatttttt
      721 tagaaaaata ctgcaacgac cttcgcacgg taatcactat gttagaatcg tcgcaaacga
      781 tggatgctga tgtgcgggct aaagtcgaag catacaaaaa agaaatggaa tgggattttg
      841 ccatgggcga catggaaaat atcctcaaaa gtttcaatga aggcgctacc cgcattcgat
      901 ccattcttgc cagcttaagc gcattctccc ggggcaatcg cattgcacgg gaagaaatga
      961 atatacacga agcgatcgat aacacgatca atcttttgcg atacaaatcc cgtcatgtcg
     1021 tagaatttga aaccaaatat aaagctaatc cttacattat cgccaatcgc ggtgagatca
     1081 atcaagtgct gatgaatctc atcattaatg cgatacacgc cgtgcaacat aaattcgaaa
     1141 acggcggtaa aggtgcgatt tacattcata cccattcggg tgaaaacttt gtttacatag
     1201 acattaaaga taacggtgaa ggcatcccgg atatttataa aaacgaaatt tttaaagcat
     1261 tctttacaac gaaaaaagaa ggcgaaggaa ccggtttggg tttgccgatt tccaaagaga
     1321 tcgtcgaaaa acacggtggt gctatcgaat ttgaaagccg tgccggtatc ggcacttgtt
     1381 ttacagtaaa acttcctttc ggtacggacg aagaaaccga cgtgcctaaa gatacgcata
     1441 accaagggaa aaaatcgtga gtgtagcgac ggttgcatct ccgtctatga cggaacccaa
     1501 caagatcatg catgatattt tggtggtaga tgatgaagtg gataatcttg atctgctgaa
     1561 acgtacgttt cgccgggagt acaatgtgta ctcggccaac agcgctgcgg aggcgttgaa
     1621 gcttttggat gaacgcgaat ttgccgtgat cgtcagcgat cagcgtatgc cggaaatgac
     1681 gggcgttgaa atgtttcaga aagcgcgcga aaaatatccg cagaccattc ggattttgct
     1741 taccggttat accgacatca atgcgctggt agatgcgatc aacatgggtc acgtgtatcg
     1801 ctatgtgacc aaaccgtgga gtcgtgaaga aatcgtcatg accgtcaagc gcgcggtcga
     1861 gcattacgaa accaccaaac aaaatgcgcg gctcttggat gaattgaaaa tcaaaaacga
     1921 agaactggat cgtaataaca aacaactgaa acgcctggat gaacttaaaa cacggttcat
     1981 ggttatttca tcgcacgaat tgcgcacccc cgcctccatc atttccggaa atctggagtt
     2041 gcttatgtcc ggtgcaatgg gtgaagtgcc cccggaacat caggagatcg ttaccaatgc
     2101 atataaaggc acgacgcgtc tgattgattt gatcgaagat gttttgtccg ttatgcgttc
     2161 ggattcgcat aatctcaaac tcaatctttc ggcgtattca ccgcgtgagg tgctggaaga
     2221 agttatttcc agttacaggg cttttttgca tgaacgccgg caaacggtac aaaacgagtt
     2281 accggaattg accattgagg gtgatcgtga caggatgttt catgtatttg ccaacgcgct
     2341 cagcaacgcg atgaagtatt cccgcgacgg tgaaaccatc ggggtatcgg ggaaactcaa
     2401 cggcgatcat atgcgcatta catttcgcga cgcgggtatc ggtattcctc cggaagaact
     2461 ggagcgtatt tttgaaaagt tttaccaact cggtgatgcg gatcagcatc ggacgagtaa
     2521 acataaattt atgggcggcg gcagcggtct ggggcttact atcgtgcgcg gtatagtgga
     2581 agaacaccac ggtcgtgtat gggcggaaag cgaaggcaaa gataaaggcg ctgcacttca
     2641 aatcgaatta cctttgcaac aaccggtagc cggataaacg aaagagctca acgtgccgca
     2701 aagtttgtct tacgttcata gtgttcatct tgcactcacg cgccgatgcg gggcccaatg
     2761 caattattgt acgttcaaac aatcggattc gccgttgatg acgtttgacg aaatcgaaca
     2821 ggtgattcgc aaacaaaaac caaccggcat ttcaacggtg gtgttgggcg ctggtcaatc
     2881 gctagaacgc ttggcgaact taccggcgtt gtggaatgat caggggtacg catcgtttat
     2941 acactacgtg caggatgtgt gccgcatcgt actggaacat caacttttgc ctgtggtgga
     3001 tattggtccg cttacatatg cccaactgga gatgttgcgt ccgtttgttc ctacgattca
     3061 tttagctttg gagaatatca atacggactt tcgcgcgacg gtacaacaaa ataaaagcgc
     3121 cgatgacatt atggaaactt tttccgatgc gggcattctc ggcataccga ttacgaccgg
     3181 tttacttttc ggcgcgggcg agagtattga tgacgggttg gcgacgctca atgcgttgga
     3241 cgaaattcag aaacgttatc acaacctgca gtccgttacc ctgcaatatg tctatgatac
     3301 gaaacgcggg aatgcgacac gaaccgacat agaatatctg gatatgttgg taaaatacag
     3361 ccgaaaggtc atgccggatg ttgccgtgat cgtgcctata catgctcaat tacattggct
     3421 tgatgaaggc cttttgccgg atgatatcgg atttgtgttt gaaggttttg acggaatcaa
     3481 ttatgatgcg ccatttccta aattaacgga actggaacgg cagtttgcca aaaatcatat
     3541 tacgcttgtt ccccgtttgc cggtgtttcc ggattttatt gatcgcattg atgcatcgga
     3601 aagtttacgc agcgtcttag ccgactggca atccaaacgt atgtatgtaa cctatgcaaa
     3661 ggcttgatct catatgacat catttgccaa tcttcatgtc atacgccatc cgctggttca
     3721 gcaaaaactg acttacctgc gagataaaaa tacatcccat cgtgaatttc gcaaattgct
     3781 cagtgaaatc acggcattta tgatttttga aatcacacaa gatctgaaaa ccaaagatat
     3841 ccaggtcgaa acgccgcttg aaactacgac ggcgcaggta ttggccgatc acgtcgtgct
     3901 tgtgccggta ttgcgtgcag gtttgggaat gctggatggc gcgcttgatc ttattccgaa
     3961 tgctaaagtc ggtcacatcg gcctttatcg caatgaacaa acgctggagc ctgtggaata
     4021 ctatgccaag tttccgccga ctctgccgga agctacggcg atcatgctcg atccgatgct
     4081 ggccaccgga gggagctctt cggcggcggt ggctttgctc aaacgtaaaa aagcacgaca
     4141 tatcaaatgt ttgtctcttg tcgcagcacc ggaaggtgta gagcgcatgc tcaaagatca
     4201 tcccgacgtg atgatttata cagccgggct cgatcgtcaa ctcaatgaac acggctatat
     4261 cgtacccggt ttgggcgatg ccggtgatcg tctttacgga actaaatagg tttaacgacg
     4321 tatcttggat ttatcaggaa catacatacc accggatcag cagcgatcac cnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaagaaagc ggaagaggaa tctttatttt
     4501 gcgttctatt atggacgacg tacagtttat gtttgccggc gatggtacag tggtcaaaat
     4561 cgtaaaacgc tttctaccga aataatgaaa acgaaagggg ttcgtatgaa acatatattt
     4621 ttcgggttgt gcttttttgc actgaacctt tctcttgtcg cgcaggagga atttgatgaa
     4681 actgcgtttg aatccggtga tgctaaactc gggaacggct ggggtatgaa tgtccaggta
     4741 tccacggtcg gctttgttat cggcggtgtg tataactata aagtcgcaca acatacatgg
     4801 ttgacatctt cactggatct tttttgggtc aatggtgaaa acgagcagca ggtttatgat
     4861 tataataccg ggcaaaccat cagtattaat agtgaaacta ttctgatgtt gccgcttgtt
     4921 tttcagatga agcgcagact tttcccggag acaatgtcca gcaatctaag gccgtttgtt
     4981 gttggcggcg gcggtgtggt gtatggttgg tttattgaca gtgatgtcag taaaagccga
     5041 ttgcccaaac cgcaagaaaa cacgcaatac acctatacct tgatcggagg tattggcaca
     5101 gacatcggca aaccgggttc gacaggatac gggcttgatg tcaaatacca atggctgcgg
     5161 tactcgaatt acctcggcgc ccgcaaaaaa tttgataata tccaggtcgg gtttcacgtt
     5221 aactttcgtt ctaactgaac ggtatggaaa tacagatcaa acaaaaaaaa cgcgttgtcg
     5281 tcgcgatgag cggcggtgtg gacagtacgg cttctgccgc cttgctcaag gagcagggat
     5341 acgaagtcat cggcgtaacg atgaagttgt ggaattacga tgaggtaggc ggcggcgaga
     5401 tcaatcggga aagcgggtgt tgctccattg acaccatgca cgatgcgcgt gtggtatgcg
     5461 aacagttagg cgtaccgcat tatgtgtggg atttgagcga cgaatttggt cgcgccgtga
     5521 ttgataactt tgtcaatgaa taccttgaag gtcgtacgcc caatccctgt gtgatgtgca
     5581 ataaacatat caaatgggga acgtttctca ccaaagctct acacctcggc gccgattttg
     5641 tagcaaccgg gcattacgcg cgatcagttt acgatcctgt cagcggcaaa tatactttgc
     5701 gcaaaagtta caaccgcaaa aaagatcaat cctatgcttt atggggtatt cgtcaaaaag
     5761 cgctggcgat gacacttttc cccgtcggtg aaatgtcgga caaagaagag gttcgcgaag
     5821 tcgcatcacg cctgcatttg cgaacggcga agaaaaaaga aagtcaggaa atttgtttta
     5881 tcacggataa taattacaac cgttttctca aagagaaaat aagcggcctt tccgaaaagc
     5941 ttgcgcacgg aatgatcaaa acccatgacg ggcaagtcgt cggtgaacac gacggatatg
     6001 ctttttatac gatcggtcag cgtcgtggtg taggtgtcgc gatgaatgaa cctgtatatg
     6061 tcacggagat cagcgccaaa gataacacca tttacgttgg tcgtaaggat gatcttttgg
     6121 caacggggct tactgcgaca caggtcaatc tttgccggca tgaagcgatc acggaagcca
     6181 tgcctattac ggccaaaatc cgatataacg acgtcggcca cgaaggcgtt ctcacactca
     6241 acgaaatcgg tgaaacccag gtaattttta atcaacccca acgcgccatt acgccgggcc
     6301 aatcggtcgt attttatgac ggtgatgaag tgatcggcgg cgggatcata gatcgtgtac
     6361 tcaaagaaac gctgcaaccg gtttaagata gcatgtccac tcatcatacc ataaccaaag
     6421 tcacgacaga acagttgcgt cgcatgaaac aaagcggtga aaagatcagc gcattgacgg
     6481 cttacgacgc gatcatggct tctcttttgg atgaatcggg aatggatatg atcttagtcg
     6541 gcgactcggc cagtacggta tttgccggca atgatacgac attgcccatg acgatggagc
     6601 agatgctgta tcatgtgcgt gtcgttacca agtcggtcaa acgcgcattg gtcgtagcgg
     6661 atatgccgtt tatgagttac catgtgagcg tcgaagaagc tattcgtaat gcgggccgtt
     6721 ttatgcagga aggtcacgcg gaggccatca aattggaagg cgggatcgag tttttaccaa
     6781 cgatacaacg attggtcgaa atcggcatac ccgtgatggg tcacctcggg cttacgccgc
     6841 agtcaattca taaatttggc acgtaccgta cacgcggcaa acaaccggat gaagctaaaa
     6901 aaatcatcag tgacgccaaa gctctggaaa aagccggcgt gtttgcgctt gttctcgaaa
     6961 aagttccggt aactttagcc aaaaaaatat cggcatctat tactataccg accataggta
     7021 tcggcgccgg gccgcattgt gacggccaga tattggtcac gcatgatatg cttgggttat
     7081 atgaaaaatt tcacccgcgg tttgtgcggc gctatgccga gcttggtaaa gctatgcgca
     7141 aagcctttgc ttcctatcat caggatgtaa agagcaaccg atttcccacc gacaaagaaa
     7201 gctattcgta gtagtttttt taaatccgtt aattttttca aaaaaaagag tgaaaacggt
     7261 taattttcac tctgttaatt tttgttatat gttgtttgtt taaaacaagc gcgccatgct
     7321 catcc
//
LOCUS       DAOQGF010000145         7248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5018_length_7248_cov_5.416933, whole genome shotgun sequence.
ACCESSION   DAOQGF010000145 DAOQGF010000000
VERSION     DAOQGF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5018_length_7248_cov_5.416933"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(608..3214)
                     /locus_tag="PK937_14180"
     CDS             complement(608..3214)
                     /locus_tag="PK937_14180"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33770.1"
                     /translation="MKISVICLLFLTCASKIGFTQEYMKLEPLFKLKDGWEINGYSPD
                     GKYVVVNKSNDYKLININNGEKIVDLSWKYVFSADSRFIAHGTEKINIFDITNSAEYQ
                     SPPVENSHQENIAIDPDRKFLAYHVVTNDYEDFRIGIWDFVNGKMKKEINLKATKLDG
                     KVLIYTRSYFFFTPDGRYLFAFLKRTDYGGGVDRGWSLVVFSTDNWKTYATLDWGNVI
                     EDNPKPIFFSEDGSIMVFSTQLSIHFYSFAENKFFNSIRVANFKETSGKYFRSNNYYA
                     ISITSKALAVLNPSNPSNVDVHALLSGSKLYTILCNFDTSGGGANYFINPVFSFSFSR
                     DGNLLAIQGWKFNSDSTKALNDRNRYAYMNSPYRGNYEYKDGRYYNRLQSIYKVADGK
                     LITHIRSTEIDDESIGSQPEWFDDSAILNGQYICNKGELYSIKNGKNLQQVDWRWYTF
                     PNDRVAVESNYKHNGHYLQLYRASYSDHHELSLMLDKPNFQQSFSMMSGAEVKEKELK
                     KALKNPEVTDLDLHGQNISKIGKEIGNLTNLRHLYLNLNRLKRLPDEVGNLRELQYLN
                     LSYNSLSELPASIGNLHKLRVLYLSGNPISNSELLKLKGVLPNTDVVYIPVDYFSNAI
                     PSPLTDADEEAISALKAKPAQLFKEDEYGLSSYSEIGRIFEEYGDFERAFRAYYMGMS
                     MGSGGDAYALGNLIELHSNLKEHKDKVSAALPYFNKACSLGFEDGCTAAKNSREHLQS
                     VTKHEERMRQMWKDFSLEATSTLSQIAVTVVTKGTNQQTINQSAASAGNEGSTDCSTC
                     DNDPTYLRLKKEAESCLSCSYCAAVRLLQCYIEKGCYKEAGANTSVTLQELKRRIEEN
                     RNNAKKMGNICN"
     gene            complement(3367..3762)
                     /locus_tag="PK937_14185"
     CDS             complement(3367..3762)
                     /locus_tag="PK937_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33771.1"
                     /translation="MKSLMRSLNYAVIGLAAISAFANDVFSQASYDYVSEYSGVEFYS
                     RWKKDPYTNDSHLNVKVINTSQETKRISFAIQWLDREGRFIEKSTNTGFTIRPGQTKA
                     GDLDGLYWYVPKGYSSHNVEAKLVDIEIR"
     gene            complement(3977..5143)
                     /locus_tag="PK937_14190"
     CDS             complement(3977..5143)
                     /locus_tag="PK937_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33772.1"
                     /translation="MRRSQKQFYFIALLTLYGYQVPLLSQVAILGYTTKFNFDGGFEY
                     NTWSPHKTNELKYDIEGFRIGYVRGELVHDIPFIPDMKFNWETNFGAKHQEEILKAHN
                     SMSKLEQGYEKMNAILSFFPESKIKKEGDEKVEMKNRDGSVRKYHNFEISYTKETFYI
                     GVTPATEGLNYAAYASGKTYGFPYGTSISQFTKFEEISATFYTDGKLYIFWIINAFFS
                     AAADRESAFSDESRIFQFPEADSRIGVFYSTFQKPYSVNQVLTQGEVINSPNTIYNAR
                     FQAAGIIDEISSPAQSSLIARWTNRFGYSWINLRKKEDLEDTNSPGFFYFGTEAELGF
                     RIHFNKFSLNLSASGNFGFMQGLNVDTEAEQFYATSFINNDLLLKYKGSLQYIF"
     gene            complement(5765..>7248)
                     /locus_tag="PK937_14195"
     CDS             complement(5765..>7248)
                     /locus_tag="PK937_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH33773.1"
                     /translation="IIPNTVIGSRGTSYYLRLSDGVNVVFSPAGAQSQANVHNIEVTL
                     PSAQADGFQPTGKTAKYYRLISIPFELKDNNPISLFANFGSFDSLSGGWSLFGWNGTD
                     YVRADLNGFPSIKPGRGYMFITSMAEKIRTDSVKTTPVNSSFPITVTSERWTCIGNPY
                     DFNVAWDSVGDRDHVNTPIWYDGTENDWKVANVLEPWKGYFVFTTERTRDIKIPAKES
                     GLAKRIEQNTFKKYQSGEWQIQIKTCGEGLNDQYNFVGMFSRASDHYDLEDRVESPRH
                     PGNEFIKLYFDHSDWPEHAFRYGSDFRPIRSDGDVWDFDVNALTSAEKIPLNFEQLEN
                     VPPDFEIILLDKDLKITRDLRKEPGYVFPAQRNVQFTRHFRIVIGKKDFVSRNLNGVS
                     VTPEKFDLSQNYPNPFNPTTGIKFSLASYSRVRLTIYNMLGQEVKTLVNENLQAGYQT
                     VLWDGRNAAGQAVSSGVYLYRLQAEALDTKQRYTKTRKMLLVK"
BASE COUNT         2024 a         1580 c         1378 g         2266 t
ORIGIN      
        1 gatttatacc ccggccttgt ataaatgcct gctctactat agatgaacgc atatccatac
       61 tgtgtttttt ttctagcctt gctacgcgtg caggaaagtt gcggagagaa ctgccgcggt
      121 aaaaattgtg aaaagtaatt tcatagattc tcctctattt atttacatct ttcaagctct
      181 ggccttggct ttgttttttt tgattccttt catcattatc taaggcaaaa ccatacaacg
      241 cgcttgtaga gaccattgat ccgtcttcgc acttacaaaa gccgtattga tttcgaagag
      301 aatcttcccc cgtcacgttg gtgcctccca taaaattaca taccaccgcg ccagaattgc
      361 caatccatcg tgtggaatcg acgccctggc atgttgctct gaggagaaga cgataagcat
      421 cgcaatccaa cgtctcattc ttttcacaat ttaccgaaat gagaatccta ttcttcggat
      481 aatatgtcat ggtctcgtaa tgaaatccct tctcttattt ccaaacctaa aagtttgata
      541 ccgggcggaa tctggttgag ccgcaagttc ttggctaaag aaacataatt caaaaagaac
      601 agccacacta attacagatg ttacccattt tttttgcatt gtttcgattt tcctcgattc
      661 ggcgtttaag ttcctgtaga gttactgaag tattggcacc tgcttccttg tagcatcctt
      721 tctcgatata gcattgaagc aatctgacgg cggcgcaata ggaacacgac aagcaggact
      781 ccgcttcctt tttcagccta agataggtcg gatcattgtc gcaggtcgaa cagtcggtcg
      841 atccttcatt tccagcagat gcggctgatt ggttaatcgt ttgctgatta gttccttttg
      901 tgactactgt tactgcaatt tgggagagcg tggaggtagc ttcaagactg aagtctttcc
      961 acatctgcct cattcgctct tcgtgttttg tcacggattg gagatgctct ctgctgtttt
     1021 tggccgcagt gcatccatct tcaaagccga gagaacaagc cttgttgaag taaggaaggg
     1081 cggcggagac cttatcttta tgttccttca gatttgaatg caactcaatt aagttaccca
     1141 aggcgtaagc atcacctccg ctacccatac tcattcccat gtagtatgcc cgaaatgctc
     1201 tttcaaagtc gccgtattct tcaaatatcc tacctatttc cgaataagat gatagaccat
     1261 actcatcttc cttaaaaagc tgtgcgggct ttgctttgag cgcgcttata gcctcctcat
     1321 ccgcatcggt tagcggagaa ggaattgcgt tgctgaaata gtcaacagga atgtaaacga
     1381 catcagtatt tggaagcacc cccttcaact tgagcaattc actgttagag atggggttgc
     1441 cactgagata cagtactcgc aacttatgta gattaccgat tgaggcaggc aattccgaca
     1501 aagagttata ggatagattc aaatattgca gttccctgag gttccctact tcatctggaa
     1561 gccgctttag tctgttgaga ttcaaataga gatggcgcaa atttgttaag tttccgattt
     1621 cctttccaat cttactgata ttttgaccat gtaggtcaag atcagtgacc tcgggatttt
     1681 ttagtgcttt tttcaattcc ttctctttga cttccgcgcc cgacatcatc gaaaatgact
     1741 gctggaagtt aggcttatca agcatcaacg acaattcgtg gtgatcggaa taagaagccc
     1801 gatacaactg taagtagtgg ccgttatgtt tatagttact ttcaacagca acgcgatcgt
     1861 tggggaatgt ataccatcgc caatcaacct gttgtaaatt ctttccattc ttaatggagt
     1921 ataattctcc cttgttgcaa atatattgac cattcaaaat agctgaatca tcaaaccatt
     1981 ctggttgaga gccaatcgat tcgtcatcga tctcagttga tcttatgtgc gtgatcagtt
     2041 tgccatcggc aaccttatat attgattgca gtcggttata ataccgtcca tctttatatt
     2101 cataattccc acgataagga gaattcatgt atgcatatct attcctatca tttaacgctt
     2161 tcgttgagtc ggagttaaat ttccagcctt gtattgccaa aagattcccg tctcgagaaa
     2221 aactaaaact gaataccgga ttgatgaagt aattagcgcc tcctccactt gtgtcaaaat
     2281 tgcacagaat agtgtataat tttgagccag acagtaaagc gtggacatct acattggacg
     2341 gattgcttgg atttagaacg gccaatgctt ttgacgttat agatatggca taataattat
     2401 tcgatcgaaa atatttgcct gatgtttcct taaaattagc gactctgata ctgttaaaaa
     2461 acttattttc tgcaaacgag taaaaatgta tgcttaattg ggttgagaac accattatgc
     2521 taccatcttc agagaaaaaa ataggtttcg gattatcctc aattacattc ccccagtcga
     2581 gagtagcata tgttttccaa ttgtcggtcg aaaagacaac caatgaccat cctctgtcaa
     2641 caccaccgcc atagtccgtt cttttcaaga acgcaaatag gtatcttccg tccggcgtaa
     2701 agaaaaaata cgaacgagtg tagataagca ctttaccatc caatttggtc gctttaagat
     2761 taatttcctt tttcatttta ccgttcacaa aatcccagat tccaatacga aagtcctcat
     2821 aatcattggt tactacgtgg taagctaaaa atttgcgatc cggatcgatt gctatatttt
     2881 cctggtgaga attctcgacg ggcgggcttt gatattctgc actatttgtt atgtcaaaaa
     2941 tattaatttt ctccgttcca tgagcaatga atcgggaatc agcgctaaaa acatacttcc
     3001 aagacaaatc aacgatcttt tctccattgt taatatttat cagtttgtaa tcattggatt
     3061 tgttaactac tacatactta ccatctggag aatacccatt gatctcccat ccgtctttca
     3121 gtttgaaaag cggttcaagt ttcatgtatt cctgcgtaaa gccaattttg gaagcgcaag
     3181 ttaaaaaaag aaggcaaatt acactgattt tcatattgta ctccgagtta aattgatcgt
     3241 gtgaatatca attatgtata gcgagtttcc attctctttc atgtttccgc ttcgcaaatt
     3301 tctgatagaa tgaagttcaa ggcgtaagcc cgttactgta ggagaagcgg acatacaact
     3361 gacttctcac ctgatttcaa tatccaccaa tttggcctcc acgttatggg aagagtaccc
     3421 tttgggaacg taccaataca gcccatccaa atcaccagcc tttgtctgtc ctggtcgtat
     3481 agtgaaaccg gtattagttg atttttctat aaaccgtccc tcccggtcaa gccactgtat
     3541 agcaaaagaa atgcgtttgg tttcctgaga tgtattgatg acttttacgt tgagatgcga
     3601 atcattagtg tacggatcct tcttccatcg cgaatagaat tcaactccgc tgtattcaga
     3661 aacatagtca tagctggcct gtgaaaaaac atcgttggca aatgcggaaa tagccgccaa
     3721 cccgatgaca gcataattta agctacgcat aaggcttttc atgattcgta cctttcatgt
     3781 gtgttaatag tggatgttcg cgcacgtgat taccattgat ttaaaggatt cttattgaac
     3841 cgttatggaa cctccagatt atatcgaata agcttcgagg catcttctaa taacccgcac
     3901 tgccgcattc taagcaacgg gttaattggg taccattaca tgatgtacca acgccgacta
     3961 ctcaatatga cctcttctag aaaatatatt gtagcgatcc tttgtatttc aggagtagat
     4021 cgttgttgat gaaacttgtt gcgtaaaatt gttccgcctc ggtatctacg ttgagacctt
     4081 gcataaaccc aaaattgccg ctggcagaga gatttaagct aaacttgttg aaatgtatcc
     4141 tgaatcccaa ttcagcttcg gtaccaaaat aaaaaaaacc aggactgttg gtgtcttcga
     4201 gatcctcttt ttttctgagg ttgatccagc tgtatccaaa acgattggtc catcgcgcaa
     4261 ttaacgatga ctgcgcaggg gatgatatct catcgataat accggcggct tgaaatcgtg
     4321 cattatagat ggtattagga gaattgatca cctcgccctg cgtcaacact tgatttacgg
     4381 agtagggttt ttgaaacgtt gaataaaata caccgatccg actgtcagct tcggggaact
     4441 gaaatattct cgattcatca ctaaatgcgg attcacggtc tgccgccgcg gaaaaaaaag
     4501 cattgattat ccagaaaata tacagtttac cgtctgtata aaaagtggcg ctgatttctt
     4561 caaatttagt aaattgtgaa atacttgttc cgtatgggaa accgtacgtt ttgcctgaag
     4621 cgtatgccgc ataattaagc ccttcggtag cgggggtaac tccgatgtag aacgtttctt
     4681 ttgtataaga tatctcaaaa ttgtgatact ttctaacgct tccatcacga tttttcattt
     4741 caactttttc atccccttct ttttttattt ttgattccgg aaaaaaagag agaatagcat
     4801 tcatcttttc gtagccttgc tctagcttac tcatagagtt gtgagctttt aatatttctt
     4861 cctgatgttt tgctccaaaa ttagtttccc aattaaattt catatctgga atgaaaggaa
     4921 tatcgtgtac caactcgcct cgaacatagc caatccgaaa gccttctatg tcgtacttca
     4981 actcatttgt tttgtgcggg ctccatgtgt tgtattcaaa tccgccatca aagttaaatt
     5041 tagttgtata gccaagtata gcgacttgac ttaatagcgg aacttgatat ccatatagag
     5101 tcagcaacgc gatgaagtaa aattgtttct gtgatctacg cataagcttt cctccttcta
     5161 tttttcattc taaacacttg gaattacttt gaatatctgc agatagtttt ttttaaaaca
     5221 gtctatccat aagaaacata tagcatctga acataacttt acgcaaaact gattggattt
     5281 aatgtgtcaa tctcattcct tgtacattca tcctatgaag ttaccgctca cagcaaaacg
     5341 aggaagaagt atgcaaaaaa ataaccctcc ccgaacctta ctggtttaag ttcaattttt
     5401 tggtttctga ttattcacac ggcaaacccg ctcgtgtttt tcgatatact tttagtaaca
     5461 tgattttgtc ctcctaaaac atttatcagt tatccagtta tcagtcttca gtgatttatg
     5521 attaagtgga atcaatgtga taaaaatatg tttttgcctg tttctctttt agacgctttt
     5581 ggaattaaaa tcataaccaa acattaaaca actacacgcc ttctctccgt gtttttttct
     5641 gaaatcgtta gtattaaacc gatatatacg agtcatctat tatcggcatg ccgtacggaa
     5701 ttattgtgtt tgccataacc tgtggatcaa catgatcgca cgacgtaaac cggtaatagt
     5761 catcttactt caccaatagc atcttgcggg ttttggtata tctctgtttg gtatccagcg
     5821 cctccgcctg caggcgatac aaatacacgc cgctcgatac agcctgcccc gcagcattac
     5881 ggccatccca caacacggtt tggtatcccg cctgtaaatt ttcgtttacc agcgttttca
     5941 cttcctgacc caacatgttg tatatcgtca atcttactct ggaatacgac gccaatgaaa
     6001 acttgatccc ggtagtcgga ttaaagggat tgggatagtt ttgacttaga tcaaattttt
     6061 cgggagtgac cgatacgcca ttcagattgc gcgacacaaa atcttttttt ccaattacaa
     6121 ttctgaaatg cctagtaaat tgaacattcc tctgtgcagg gaaaacgtag cccggttcct
     6181 tcctaaggtc gcgcgtgatt ttgaggtctt tgtcgagtaa aatgatttca aaatctggcg
     6241 gcacattttc taattgttcg aaattgagcg gaatcttctc agccgaggtt aaagcattga
     6301 catcaaaatc ccacacatcg ccgtcggagc gaattgggcg aaaatcagaa ccataacgaa
     6361 atgcatgctc cggccaatcc gaatggtcaa aatagagttt gatgaactca tttcccggat
     6421 gacgcgggga ctcgacccga tcttctaaat catagtgatc ggatgccctt gaaaacattc
     6481 cgacaaagtt gtattggtca ttaagccctt cgccacaagt cttaatttga atctgccatt
     6541 cgccggattg atattttttg aaagtatttt gttcaattct ttttgctaac ccgctctctt
     6601 ttgccggaat cttgatatca cgtgttcttt cagtagtgaa aacaaaataa cctttccaag
     6661 gttcaagaac atttgcaacc ttccagtcat tttctgttcc atcataccaa attggcgtat
     6721 taacatggtc tctatctccg acactgtccc atgccacatt gaaatcataa ggatttccaa
     6781 tgcacgtcca tctctcacta gtcacagtaa ttggaaaact tgaattaact ggcgtcgttt
     6841 ttactgaatc agttctgatt ttttctgcca tagacgtgat aaacatatag ccccgccccg
     6901 gtttgataga aggaaaacca ttaagatcgg ctcgtacgta gtcagttcca ttccaaccaa
     6961 aaagactcca tccgcccgaa aggctgtcaa atgagccaaa attagcaaac agcgaaatcg
     7021 gattattgtc ctttaattcg aagggtattg atatgagacg atagtatttg gcagttttcc
     7081 cggttggttg aaaaccatct gcttgcgccg aaggaagagt gacctcaata ttgtgaacat
     7141 tagcttggga ctgcgctcct gccggactaa agacgacgtt cacgccgtct gaaagccgca
     7201 gatagtaact tgttcctcgc gatccgatca ccgtatttgg aataatac
//
LOCUS       DAOQGF010000146         7176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5094_length_7176_cov_5.564949, whole genome shotgun sequence.
ACCESSION   DAOQGF010000146 DAOQGF010000000
VERSION     DAOQGF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5094_length_7176_cov_5.564949"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /gene="aroE"
                     /locus_tag="PK937_14200"
     CDS             complement(<1..810)
                     /gene="aroE"
                     /locus_tag="PK937_14200"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNH33774.1"
                     /translation="MINDWITGTTTLYGIMGYPVSHSLSPIIHNTAFHYLKIASCYVP
                     WNVFPEKFETALRGAQALGIKGLNITIPHKETALYLCDMVSDEARAIGAVNTIVFTEN
                     LIRGYNTDAYGFFRALQTCNIDQKNKNILILGAGGGARAVAYALGNFFPESTIFISNR
                     TTEKANKLVKHFNNMFEKNDLFRSVTASDMKTIQFSLVVNTTPPQSEHFDEQLFKRYT
                     GTAIDIAYSINDTPFLSMAKKHQWIISDGLEMLLFQAAQSFNLWTNKLMPID"
     gene            complement(803..1531)
                     /locus_tag="PK937_14205"
     CDS             complement(803..1531)
                     /locus_tag="PK937_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33775.1"
                     /translation="MQGEADSASALWTKKVWDALGGKQRFDEVRYISFDFSVISDGKE
                     VAKHRHVWDKHSNRYRVEGKNRAGEVYSILFSDLNEKKGTAWVNGQIQSGDALKKQLD
                     YGYGRYINDTYWLIMPWKWRDHGVRLRYDGTQKDSITGKALQTLHLSFAQVGLTPGDQ
                     YWVMIDPTTSRMESWKFLLQGLGAEKGHYLWQDWISVNGISFSTTKISDDEKRKILTN
                     NIRTYEVLSDSVFLFSDYTISARD"
     gene            complement(1636..2373)
                     /locus_tag="PK937_14210"
     CDS             complement(1636..2373)
                     /locus_tag="PK937_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33776.1"
                     /translation="MLRGLITCLTALSLSFVVSCSSGSKMYQHSDLQNKQSGLSGRSA
                     TVHIAGIDFDCKIVGLNKDVLIVQQSTLRSEIPLDAISKLEVRGSMSHKTKMLIGGGA
                     GLLVGTGAGLIVGPKVSETKGAKSLAGPLPMLMIIGGTVGGGFLGSSLAKNDEYVFNA
                     EIKPYSIHTEIGTEATPAELVAYGIFDDLSTGADEKILQVQVFQLKDNRYFIMYDESY
                     LGAYRVGGKIVNEEYIKKQAEKVSKKQ"
     gene            complement(2403..3341)
                     /gene="trxB"
                     /locus_tag="PK937_14215"
     CDS             complement(2403..3341)
                     /gene="trxB"
                     /locus_tag="PK937_14215"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNH33777.1"
                     /translation="MTLSAEVKNVIIIGSGPAGFTAAIYASRANLNPVIFEGHQPGGQ
                     LTITTEVENYPGFPKGVMGPEMMEMLREQAKRFGTECVFRSIDKVDFSQRPFKLTDDS
                     GEVHLAKAVIIATGASAKLLGFPKEKIMMGHGVSACATCDGFFFKGKHIHVVGGGDTA
                     MEEAGYLTKFAASVTIIHRRNTLRASKIMQERVQKNEKVKFIWDSEVVDILGDENTQI
                     QGLKLRNTVNGALSEVPSQGLFLAIGHQPNTHLFNGILDMDESGYLITQGKSTKTNIE
                     GVFACGDVQDQTYRQAITAAGSGCMAAIDAERFLGH"
     gene            complement(3351..3827)
                     /locus_tag="PK937_14220"
     CDS             complement(3351..3827)
                     /locus_tag="PK937_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33778.1"
                     /translation="MQLRDQDRLKDIYLNTDFDSFRNGFMRNFTYSNVSALIIWLYDY
                     VVHPNNDFFYWFRLFGVSTSIYFSAWFVEMLWWKFKGANHETPFMVEAYTERIVLSSI
                     AGGIFFPLAMLLCRLTEPELIWPWLPLVGFSVALFVQIFIQWSQLQKLRKLSLTNL"
     gene            complement(4044..6191)
                     /locus_tag="PK937_14225"
     CDS             complement(4044..6191)
                     /locus_tag="PK937_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH33779.1"
                     /translation="MKNSLLCAVMIAHFFPFLLIGQNKDSTRIYNLEGTTITSTRVPE
                     SIIEVPLAVSVIDRKEFDTKRGFGMNDAMIHIPGVLAQSRSGGTDLRISIRGFGTRGA
                     GDRSNAGTSRGIRVLLNGLPLTEPDGRTALDLIDMNMVDRMEVVRSNASSVWGNAGGG
                     IVDISTLPSNNGRFAVMQAKTGSFGLREWHARTGTTIGSTQFYAGYTQNSYDGWRANS
                     QAERRILTMGLQSSVTEQTTLTALLVGGHNQFSIPGPLTKQQYDSLPTQANPAYKTQR
                     ERRNNKILYMGLGLQHNIDDENQISGMAFVNPKVLHRSERNTYRDFTRYHIGGNTMYR
                     HNHEWGALKTITLVGGDLAYQDGAILFYPLGADGNRVYANPVQDKKEGANNFGLFIQN
                     EFKFSERWTLLTGLRYDDVTYHSQNFLKTKPGLKSKSFKHWTPKAGITYLVTPTHSLY
                     ANMGGGVEVPAGNETDPSNTAGQYSDTTYAFNPLLKPITSVTGEIGTKNVMVTDGFLK
                     GITYDAALFWIQVTNDVIPYRSGRFYMTAGKTQRIGLEISEKTDFAKGFSLKTSATMS
                     KNTYEKYRIDSSLINTGSAGKFSDYSDNKQAGVPDFYYSVSGRWAPDFVKWIFAELTI
                     QGIGSYYTDDANTIKVPAYTVLNASIGIGEPIRLSEHYTIRGFISVNNLMDKKYAGST
                     FINPDLNSVDKKPMYLEPGLPRNFAASISLTWE"
     gene            complement(6288..6695)
                     /locus_tag="PK937_14230"
     CDS             complement(6288..6695)
                     /locus_tag="PK937_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33780.1"
                     /translation="MRRAFLIKHTLVLPVLITTVFAVSQPYALLGLLVSPLQVVLYWD
                     APDNDEDTFITKSHPDSPHSVFIKEESKRDADQRFKLKLKPIHFTVVSPFIGSYADEA
                     FTLIWSLLPITPYPEIPAFTFHPPNFSTLHRVQ"
     gene            6804..6874
                     /locus_tag="PK937_14235"
     tRNA            6804..6874
                     /locus_tag="PK937_14235"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6836..6838,aa:Gln,seq:ctg)
     gene            7011..>7176
                     /locus_tag="PK937_14240"
     CDS             7011..>7176
                     /locus_tag="PK937_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNH33781.1"
                     /translation="MNLGMPEIIVIAVAVLILFGAKKIPEFAQGLGKGIREFKRAMNG
                     AEDEIKKGLSD"
BASE COUNT         2096 a         1573 c         1402 g         2105 t
ORIGIN      
        1 atctatgggc attaatttat tggtccatag attaaatgat tgagcggcct gaaacaaaag
       61 catttccaaa ccatcgctta taatccattg gtgttttttg gccatagata aaaatggcgt
      121 atcattgata gaatatgcga tatcaatagc tgtacccgta tatcttttaa ataattgctc
      181 atcaaaatgc tcagactgcg gcggcgtggt attaacgact aacgaaaact gaattgtttt
      241 catatctgaa gccgtcaccg atctaaacaa atcatttttt tcaaacatgt tattaaaatg
      301 tttgacaagt ttgttggctt tttctgtagt acgattagat ataaatatcg ttgattccgg
      361 aaaaaaatta cccaacgcat aggccaccgc gcgcgctccg ccgccagcac ctaaaatgag
      421 aatgttctta tttttctggt ctatattgca tgtttgtaat gctcggaaaa atccataggc
      481 atccgtgtta tagcctctga tcaagttctc tgtaaaaaca atcgtattga ctgctccgat
      541 ggctcgggct tcgtccgaca ccatgtcgca tagatataaa gctgtttctt tatgagggat
      601 ggtaatatta aggcctttaa tgcccaacgc ttgagcgcca cggagagctg tttcaaattt
      661 ttcaggaaaa acgttccatg gaacgtaaca ggatgcgatt tttaaataat gaaaagctgt
      721 attgtgaata atcggcgata aagaatgact gaccggatac cccataatgc cataaagggt
      781 cgttgtacct gtaatccaat cattaatcac gggcagaaat cgtataatct gagaacaaaa
      841 agacactatc ggacaaaact tcgtatgtac gaatattgtt ggtaagaatt tttctttttt
      901 cgtcgtcgct tattttggta gtagaaaaag aaattccgtt tacggaaatc caatcctgcc
      961 ataggtagtg ccctttttct gcacccagac cctggagtaa aaacttccag ctttccattc
     1021 ggcttgtagt gggatcaatc ataacccaat actgatcgcc gggcgttaaa ccaacttgag
     1081 caaaagacaa atgcagcgtt tgtaatgctt tgccggttat tgaatccttt tgagtgccgt
     1141 cgtaacgtaa acggacacca tgatcgcgcc acttccaggg catgatcagc caatatgtgt
     1201 cattgatata acgtccatat ccgtaatcca gttgcttctt caaagcatca ccgctttgga
     1261 tttgaccgtt tacccatgcc gtcccttttt tttcatttaa atctgaaaat agaattgaat
     1321 aaacttcacc tgctcggttt ttaccttcga cacgataacg gttactgtgt ttatcccata
     1381 catgacggtg ttttgccact tccttaccgt ctgaaataac cgaaaaatca aatgaaatat
     1441 atcgcacctc atcgaaccgc tgtttgccgc ctagcgcatc ccatactttc ttagtccaca
     1501 gcgcgctggc agaatctgct tctccttgca tatcagattc actcatcaca gcgttagaaa
     1561 tggtttccag aggagatggt gttttaggtt tacaaccaat aagaaaacct aaaattaaaa
     1621 taagacgata tatcattatt gcttcttact gactttttca gcttgttttt taatgtattc
     1681 ttcattgacg attttaccgc ctacgcggta cgcgccgagg taactttcat cgtacatgat
     1741 aaaatatcgg ttatctttca attgaaacac ctgaacttga agtatttttt catcagcgcc
     1801 cgtagatagg tcgtcaaaaa tgccatacgc aacgagttca gcaggtgttg cttctgtacc
     1861 tatttctgtg tgaatagaat acggtttgat ttcggcatta aatacatatt catcattttt
     1921 tgccagtgaa gaaccgagga aaccaccgcc tacggttcct cctataatca taagcatagg
     1981 caaaggtccg gctaacgatt tagctccttt agtttcagaa actttcgggc ctacaatcag
     2041 gcctgcgcct gtaccgacta aaagaccggc gccgccgccg atgagcattt tagtcttgtg
     2101 tgacatggaa cctcgaacct ccaacttaga tatcgcatca agcggaattt cgcttcgaag
     2161 tgttgactgt tgtacaatca aaacgtcttt atttaagccg acaattttgc agtcaaaatc
     2221 tataccggca atatgtactg tagcggaacg accggaaaga ccggattgtt tattttgcag
     2281 atcgctgtgc tgatacatct tggagcccga actgcagctg acaacaaacg aaagcgatag
     2341 agccgttaaa caagtaataa gacctctcaa cattttgatt ctccagatat gtgtttttat
     2401 tattaatgac ctaaaaatct ttcagcatca atggcggcca tacatccgga accggcagct
     2461 gtgattgctt ggcgataggt ttgatcctga acatcgccac acgcaaaaac accttcaata
     2521 tttgtttttg ttgatttacc ttgcgtgatc agatagccgc tttcatccat atcaagaatg
     2581 ccattgaata aatgcgtatt gggttgatga cctatcgcta aaaacaaacc ttgtgaaggt
     2641 acctcggaaa gcgctccgtt gaccgtgttc cgaagtttaa ggccttgaat ctgagtattt
     2701 tcatcgccta aaatatccac aacttcgcta tcccagataa attttacttt ttcatttttt
     2761 tgaacgcgct cctgcatgat tttggatgct ctgagcgtat ttctgcgatg aatgatcgtt
     2821 accgaagctg caaatttggt caaatatcct gcttcctcca tagctgtatc gccgccaccg
     2881 acgacatgga tgtgtttgcc tttgaaaaag aatccgtcac atgtcgcaca agccgacaca
     2941 ccatgcccca tcattatttt ttccttggga aatcccagca atttggcgga tgctcccgta
     3001 gcaatgatca cggcttttgc taaatgaacc tcaccgctat cgtcagtaag cttaaaaggc
     3061 ctttgagaaa aatcaacttt atctatgctc cgaaaaacac actccgtacc aaaacgcttg
     3121 gcctgttcac gcagcatctc catcatttca ggtcccataa caccttttgg gaaaccggga
     3181 taattttcaa cttcagttgt gatggtcaat tgcccgccgg gttgatgccc ttcaaaaatt
     3241 accggattta ggttggctcg tgacgcataa atagccgccg taaaacctgc cggaccggag
     3301 cctatgataa tgacgttttt aacttccgca ctcaaggtta tactccatat ttataaattg
     3361 gtcaatgaaa gttttctaag tttttgcaat tggctccact gtatgaatat ttgaacaaat
     3421 aacgcgacac taaatccgac taacggtaac catggccata tcaattccgg ttcagtcagc
     3481 ctgcataaca acatagctag aggaaagaaa attcctccgg ctatgctact taagactatt
     3541 ctttctgtat aagcctcaac catgaaaggc gtttcgtgat ttgcgccttt gaatttccac
     3601 cataacattt cgacaaacca agcgctaaaa tagatcgaag tcgatactcc gaataggcga
     3661 aaccaataga agaaatcatt attgggatgt actacgtaat cataaagcca aataatcaag
     3721 gcgcttacat tggaatatgt aaagtttcgc atgaagccat tacggaacga atcgaaatcc
     3781 gtgttcagat aaatatcttt gaggcgatct tgatcacgta gctgcatgga ggtaatagta
     3841 tgaatttttt ttggtaggcg caatgttcaa aaaggactac aaataaataa taaaataaac
     3901 tgccccaata atttcgacca aactgtactt tctcatttta tatcaatagc aatagacgaa
     3961 atctcatcat tgcttgcaat ccttgagaca gtagctatgg gcgaagaacc attggggcag
     4021 cattgaaaac tcagtaaatc aaattattcc catgtcagcg aaatggatgc tgcgaagtta
     4081 cgcggcagac ccggttctaa atacataggc tttttatcaa cgctattcaa atcgggattg
     4141 ataaacgtcg aacctgcgta tttcttatcc ataaggttat tgacggaaat gaagcctcgg
     4201 attgtgtaat gctcgctcaa tcgtatcggt tcacctatac ctatggaagc attaagaacc
     4261 gtataagcag gaactttgat tgtgttggca tcatccgtat agtacgatcc gataccctga
     4321 atcgtcaatt cggcaaaaat ccattttacg aaatccggag cccatctgcc cgatactgaa
     4381 taataaaaat ccgggactcc ggcctgctta ttatcgctgt aatcgctgaa tttccctgca
     4441 ctgccggtat ttatgagcga cgaatcaata cgatattttt catacgtgtt tttagacatc
     4501 gtagcgctcg tttttagcga aaaacctttt gcgaaatctg ttttttcgct tatttctaag
     4561 ccaatacgtt gtgttttccc cgcggtcata taaaaacgcc cacttcgata cggaatcaca
     4621 tcgttagtaa cctgtatcca aaataaagcc gcatcataag ttataccttt gagaaatcca
     4681 tccgttacca ttacattttt agtgcctatt tccccagtaa cggaggtaat cggctttagt
     4741 aatggattga aagcgtaggt ggtatccgaa tattggccgg ccgtattaga cggatccgtt
     4801 tcattacctg ccggaacctc aaccccgccc cccatattgg cataaaggct atgtgtcgga
     4861 gtaaccaaat aagtaattcc cgcttttggg gtccaatgct tgaacgattt cgatttaagt
     4921 ccaggtttgg tttttaaaaa attttggcta tggtacgtca catcatcata acgaagccct
     4981 gtaagcaatg tccaccgctc gctgaatttg aattcatttt gaataaataa accaaaatta
     5041 ttagcgccct cttttttatc ctgaaccgga ttagcataca cccgattgcc atcagctccc
     5101 aaaggataaa ataaaatagc cccatcttga taagccagat ctcctccgac aagtgttatg
     5161 gttttcagag cgccccattc atgattatgc cgatacatag tattgccacc aatatggtaa
     5221 cgcgtgaaat cgcgataggt attacgttca gaacggtgta gtactttggg atttacaaat
     5281 gccatcccgg atatttggtt ctcatcatcg atgttatgct gcaagccgag acccatgtat
     5341 aatattttat tatttcgtcg ctcacgctgt gttttataag cgggattggc ttgtgtaggc
     5401 aaagaatcat actgctgttt cgtcaatggc cctgggatac taaattgatt atgccctcct
     5461 acgagtagcg ctgtaagtgt agtttgttca gtgactgaag attgtaagcc cattgtgaga
     5521 atgcgtcgct cggcttgcga attggcacgc caaccgtcat aagaattttg agtataacct
     5581 gcataaaact gagtactgcc tatcgtagta ccggttcgtg catgccactc tctgaggcca
     5641 aaacttcccg ttttggcctg cataacggcg aaacgaccat tgtttgaagg caaagtagaa
     5701 atatccacaa tacctcctcc ggcattaccc catactgaag acgcattgga acgaacaact
     5761 tccatacggt ctaccatatt catatcaatc agatccaacg ctgttcgtcc atccggttcg
     5821 gttaagggca atccattaag taaaacacgt atgccacgcg aagtgcccgc attggagcga
     5881 tcccctgcac cacgtgtacc aaaaccgcga atcgaaatgc gtaaatcggt tccacccgaa
     5941 cgggattgcg ccaaaacacc ggggatatga atcatggcat cattcatacc aaatccacgt
     6001 ttggtatcaa actctttacg gtcaataacg ctgacagcca gcggcacttc aatgattgat
     6061 tcgggaacac gcgtggaagt gatcgtcgta ccttccagat tataaattcg cgtactgtcc
     6121 ttattttgcc caatgagcaa aaatggaaag aaatgcgcta tcataacggc gcataataaa
     6181 ctatttttca tagtactcct acctgagtaa ataaatgaat gaaaccgtac agcaaaccaa
     6241 atatagatca tttgaattat atttgatttc accgcacgga aaatggatta ctgaacccga
     6301 tgtagagtac taaaatttgg cggatggaat gtaaaggccg gtatttccgg atacggagtt
     6361 ataggaagca gagaccaaat tagtgtaaac gcttcatcgg cataactgcc gataaatggc
     6421 gatacgactg taaaatggat aggtttgagc ttaagtttaa atcgttgatc agcatcgcgt
     6481 ttggattctt ccttaataaa aacactatgg ggcgaatcag gatgactttt cgttatgaat
     6541 gtatcttcat cgttatccgg tgcatcccaa tacaacacga cttgcaaagg ggaaaccaat
     6601 aatccaagca acgcgtacgg ctgacttacc gcaaaaacgg ttgttatcaa taccggtaaa
     6661 acgagtgtgt gtttaataag aaaagccctt cgcatgaacg aaatataaag ttatatttat
     6721 ttcgtgcaaa tatgatttta tgtttttgaa acttcaaaat tccgttgatt tttttgatta
     6781 agccagtata ttccactgtt ctttggaatg tcgtccaacg gtaggacagt ggactctgga
     6841 tccattaatc ggggttcgaa tccctgcatt ccagcacgta aatgcccgat tttgatcggg
     6901 catttacttt tattacctac aataaattgc ttgcaatttt cgacctcgtt ttctatcata
     6961 aactattgag tcaaatatgt tcaatttatc ccttttggga ggtgttttag atgaatttag
     7021 gaatgcccga aataatcgta atagctgtcg ccgttcttat tctcttcggc gcaaaaaaaa
     7081 tacccgaatt tgcgcaaggt cttggcaaag gtattcgcga atttaagcgt gcaatgaatg
     7141 gtgctgaaga tgaaatcaaa aaaggtttat ccgata
//
LOCUS       DAOQGF010000147         7121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5136_length_7121_cov_6.030710, whole genome shotgun sequence.
ACCESSION   DAOQGF010000147 DAOQGF010000000
VERSION     DAOQGF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5136_length_7121_cov_6.030710"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /locus_tag="PK937_14245"
     CDS             complement(<1..2090)
                     /locus_tag="PK937_14245"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNH33782.1"
                     /translation="MIADFHSSLQELRTAIRSSSAPSAWIKKQIVDIKLSVVSQAASP
                     ESGFNASQALIELYDSIITEVFARHRLLESDAVCIFALGGYGRREMNLFSDIDINLVY
                     GTTAVPDAIRASVQPFIAQLWDMGLELGHSVRSIEDCVSLALQDAEIRTSLLDAHYIA
                     GDTVTADKFRQAVQNRIHTHDPQTYIQSRIQHMRARHRHHGNSERVLEPDIKEGKGAL
                     RDLHTLYWVIQTAFRISREIETSTSHTIVALRTLGDQHILSSDEIDRTEKAADFLMAV
                     RNALHMFAKRRNDRLSYSVQLQLATYFGYQDTPEAKGVEVFMRRYYSEARHISHTCIT
                     AIHKLHVKFARPAFDDARLPLENGFYLWQHDGDRTLEFNGDFGEHIRTEPRWMIRIFT
                     LAQKYNATLSDTLQATLREHHYMLDEAQITHREIIRDFLEIWRCEGQIASTLRTMHEL
                     TLLEKMVPEFGYIVAHYNYNIYHKYTTDEHLIVAVEALERLFFDEVTRDIDIKHLRQI
                     YEELTLDERYQLYWAVFLHDIGKSRGGDHSEIGVTLAGDIFRRLHYTDGVETIQFLIL
                     NHLHMEQTAFRRNLKDSETIQNFATLIGDRRRLRMLYLLTYADMSAANKNVWTEWKSL
                     LLQELFFRTDDALKTHAGESSEFSTWEELDYAGIVYDGKLSVSFTDREKYTEVIAVTT
                     DAPYRLATLCGALSV"
     gene            complement(2080..2697)
                     /locus_tag="PK937_14250"
     CDS             complement(2080..2697)
                     /locus_tag="PK937_14250"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNH33783.1"
                     /translation="MELSQFFEWIQSLDTGLIYLVIFLSGFLENCIPPIPGDMVTVLA
                     ASMVGVGRLSYFPTFLTATAGNLIGFMTMYQIGRFFGKDFFLSRNFKYFPATAFEKAE
                     AWFTRHGYRIILFNRFLSGLRSVISLFAGMTHLQKRKIIPLAFLSACMWDGVLIYAGY
                     VLGDNWKAFENLMAQYNRVVFSILGLIALILLIRWFIKRNSDHDR"
     gene            complement(2703..3584)
                     /locus_tag="PK937_14255"
     CDS             complement(2703..3584)
                     /locus_tag="PK937_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNH33784.1"
                     /translation="MIYSMTGFGKSERIFGSTSISVEMRSVNNRFLDLSWKSPRSFVL
                     KENEVREFLKRKLDRGKITVSLTIQSPDVNLASFKIDHSVFQQYASALREAAEKAGIA
                     QDVRLEHLLQYNDIFVSNNDAEAQEALWKNILTCIDEALDEMNRMRRSEGTELAKDFI
                     HRLETIETRLDKITELSKTAVTEELEKLRERVKLLLERTDFDPQRLDMELAILADKVD
                     ITEELVRFRSHNKMFRSLVEGKETQVGRKLNFIVQEMGRETNTIGSKSSQSEVIHTVV
                     SIKEELEKIREQIQNVE"
     gene            complement(3581..4570)
                     /gene="galE"
                     /locus_tag="PK937_14260"
     CDS             complement(3581..4570)
                     /gene="galE"
                     /locus_tag="PK937_14260"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNH33785.1"
                     /translation="MKILVTGGAGYIGSHAVRLLLRAGHDVVIFDNLIFGHADFAQGC
                     PLIQADLSDMAMLEKTFEQHKPQAVLHFAAYAYVGESVENPGKYYQNNVGSTLHLLEA
                     MVRHHVKKLVFSSTCATYGIPQKTPLTEDHPQAPINPYGQTKKMVEQILTDFDRAHGL
                     KSVCLRYFNASGADPDGGIGEDHNPETHLIPLVLDAAMGRRSQITVYGTDYETPDGTC
                     IRDYIHVTDLADAHIRALDFLTRENCSDFFNLGNGKGFSVKEIIAASEKVTGKNIPVH
                     YGERRPGDPAVLVGSAEKAKAILGWQPQYNDIHTIIQTAWNWHQSRFGSKRLV"
     gene            complement(4580..5062)
                     /locus_tag="PK937_14265"
     CDS             complement(4580..5062)
                     /locus_tag="PK937_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNH33786.1"
                     /translation="MHSFDIVSKINMQELTNAVNQAVKEIQTRYDFKDSKSTIELGKD
                     EIVIHSDDDFKLKAVIDILQNKMIKRAVPIKALKYGKVEEASGSTVRQHIKLQNGIEQ
                     DQAKKIVAMIKDLKMKVQASIQGDQVRVQAKDIDDLQAIIKTLKEKNFDFAMQFENYR
                     "
     gene            complement(5137..5445)
                     /locus_tag="PK937_14270"
     CDS             complement(5137..5445)
                     /locus_tag="PK937_14270"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNH33787.1"
                     /translation="MRKFKVCKESEIAEGQGRSIKVLAKEIMIYRIDGQLRAYDGLCK
                     HMRASLVSQSELKEYTLTCKWHGWQYDIRNGACLGKPDVYLTAHTIEITNGDIYVCVA
                     "
     gene            complement(5473..6642)
                     /locus_tag="PK937_14275"
     CDS             complement(5473..6642)
                     /locus_tag="PK937_14275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNH33788.1"
                     /translation="MKTIQLKPKHDHRIRNGHRWIFSNEIHDSLKSYEPGESVKITDA
                     NGYPVGVGYINPYSLIAVRILSFKPVEINRPFFLERIRNAYERRQVFYADQSCYRVVF
                     GESDDLPGLTIDRYGDYWVMQILTAGMDIHQDMIVDIIRSEFSPKAIILRNDSAVRKL
                     EHLPEEVRVAYGDCPSMVEVTIQNLLFRLDLLHGQKTGFFLDQKENHAYVRRFTSGKR
                     VLDCFTHLGGWAVHAALGGATEVIGLDISEAAIAQCRTHAEINQTTQCQFKCVDVFDE
                     LKAINDRRESFDVIILDPPAFAKSKSQVKEAIKGYREINRRAAKAIPSGGYLFTCSCS
                     QAIDPDTFRNTVFQGITAAGRQALLLEERTQPKDHPILMSMRETQYLKCLVIQII"
BASE COUNT         1868 a         1645 c         1668 g         1940 t
ORIGIN      
        1 acgctcagag cgccgcatag tgtcgctagg cggtagggcg cgtcagtggt gacggcgatg
       61 acttcagtat atttttcgcg atccgtaaac gaaaccgaaa gtttgccgtc gtacacaatg
      121 ccggcgtagt ccagttcctc ccaagtcgaa aactccgaac tttcacccgc atgggttttg
      181 agcgcatcat ccgtgcggaa aaaaagttct tgtagcaaaa gacttttcca ttcggtccat
      241 acatttttat tggccgcgga catgtcggcg tacgtcagca gatagagcat gcgcaggcga
      301 cgccggtcgc cgatgagtgt tgcaaaattt tgaatggttt ccgaatcttt gaggttgcgg
      361 cgaaatgccg tttgctccat atgcaggtga tttaaaatca gaaactgtat ggtttcgaca
      421 ccgtcggtat agtgcaatcg tcgaaaaata tcaccggcca gtgtcacgcc gatttcgcta
      481 tgatcaccgc cgcgggattt gccgatatca tgcaaaaaca ccgcccagta taactggtat
      541 cgctcatcca acgtgagctc ttcataaatc tggcgcagat gttttatatc aatgtcccgg
      601 gttacctcgt caaaaaacaa acgttccaaa gcttccacgg ccacgatcag atgttcgtcc
      661 gtggtgtatt tatgatagat gttataattg taatgcgcga cgatatagcc gaattccggt
      721 accatttttt ctaaaagcgt caattcatgc atggtgcgca aggtggatgc gatttgtcct
      781 tcgcagcgcc agatttccaa aaaatcgcgg atgatttcgc gatgcgtgat ttgtgcttca
      841 tccagcatgt aatgatgctc acgtaaggtc gcttgcagtg tatcggagag cgtggcgttg
      901 tacttttgtg caagggtaaa tatccggatc atccagcgcg gctccgtacg aatatgttca
      961 ccgaaatccc cgttaaattc taacgtacgg tcaccgtcgt gctgccagag ataaaaaccg
     1021 ttttccaacg gaagtcgtgc atcgtcgaaa gcgggtcggg caaacttcac atgcagcttg
     1081 tgaatggccg taatgcaagt atgactgata tggcgcgcct cgctgtaata ccggcgcata
     1141 aaaacttcaa cacctttggc ctcaggggta tcttgatacc caaaatatgt cgccaattgc
     1201 aattgtacac tgtaggatag gcgatcgttt cttcgtttgg cgaacatatg caatgcattg
     1261 cgtacagcca tcaaaaaatc ggcggctttt tccgtccgat caatttcatc cgaagataaa
     1321 atatgttgat cgcccaaagt acgcaaagcc acgatggtgt gcgatgtgct cgtttcgatc
     1381 tctcgtgaaa ttcggaaagc cgtctggatt acccaataca atgtatgcag atcgcgcagg
     1441 gcgccttttc cttctttgat atccggttcc aatacacgct ctgaattgcc atgatgcctg
     1501 tgacgcgccc gcatgtgttg aatgcgggac tgaatgtacg tctgcggatc atgagtgtga
     1561 atacggtttt gtacggcctg tcgaaactta tcggccgtga cagtatcgcc cgcaatatag
     1621 tgcgcatcga gcagactggt gcgaatctcc gcatcttgca aggccagcga tacacaatct
     1681 tcgatcgaac gtacactgtg ccctaactcc aatcccatat cccataactg cgcgatgaac
     1741 ggttgcacgg atgcccgaat cgcatccgga acagcggtcg taccgtacac cagattaata
     1801 tcaatgtctg aaaaaagatt catttcccgt cggccgtatc cgcccagagc aaaaatgcat
     1861 acggcatcgc tttccagaag acgatgccga gcgaatactt ctgtaataat gctatcgtag
     1921 agttcgatga gagcttgcga cgcattaaaa cccgattccg gagaagcggc ttgcgatacg
     1981 accgatagtt tgatatcaac gatttgtttt tttatccacg cggaaggagc agaagacgaa
     2041 cgaattgctg tgcgaagttc ttgcagcgat gaatgaaaat cagcgatcat gatcggaatt
     2101 acgtttgatg aaccaccgga tcaagagaat aagtgcgatg agacctaata tactaaaaac
     2161 cacccgattg tattgtgcca tcagattttc aaacgctttc cagttgtcac cgagtacata
     2221 tcctgcgtaa atcaaaacac catcccacat gcacgcgctc aggaatgcga gcggaatgat
     2281 cttacgtttc tgaagatgtg tcatacccgc aaaaagtgaa atgacagagc gtaaaccgct
     2341 caaaaaacga ttaaaaagaa ttatacggta tccgtggcgg gtaaaccatg cctcggcttt
     2401 ttcaaacgcc gtcgcaggaa aatacttaaa attacgagac aggaaaaaat ccttaccgaa
     2461 aaagcgaccg atctgataca tcgtcataaa accgattaaa ttaccggctg ttgccgtcag
     2521 aaaagtagga aagtaagaca ggcgtcctac gccgaccatg ctggccgcta acaccgtcac
     2581 catgtcgccc ggaatcggag gaatacagtt ttccaaaaag ccgctcagaa aaataaccaa
     2641 gtaaatcaaa ccggtatcga gcgactgtat ccattcaaaa aactgactga gttccatata
     2701 cgttattcga cgttctgtat ttgttcgcgg attttttcaa gctcttcttt gatggatacg
     2761 acggtgtgga tcacctcgga ctgggatgat ttggaaccga tggtgttggt ttcgcgtccc
     2821 atctcttgta cgataaaatt gagtttgcgg ccgacctgtg tttctttgcc ctcgacaaga
     2881 gaacgaaaca tcttattatg gctgcgaaat cgaaccaatt cttccgtgat atccacttta
     2941 tcggcgagta tggccagttc catgtcaagt ctctgaggat caaaatcggt gcgttctaat
     3001 agaagtttta cgcgctcacg tagcttttca agttcttcag tgacggctgt tttggataat
     3061 tcggtgattt tgtccaaacg ggtttcgatc gtttcgagcc gatggatgaa atccttcgcc
     3121 agctctgttc cttcactgcg acgcatgcgg ttcatttcat ccagcgcttc gtcaatacaa
     3181 gttaaaatat ttttccaaag cgcttcttgg gcctcggcat cgttattgga aacaaaaata
     3241 tcattgtact gcagaagatg ctctaaacgt acatcctgtg ctataccggc cttttccgcc
     3301 gcttcgcgta aggcggaagc gtattgctga aacacgctgt gatcaatttt aaacgaagcc
     3361 agattgacat ccggagactg aatggtcaat gataccgtga ttttaccgcg atcaagtttg
     3421 cgtttcagaa attcacgcac ctcgttttct ttaagaacaa aactgcgcgg tgatttccac
     3481 gaaagatcta aaaaacgatt gttgacggaa cgcatttcga cggaaatgct ggttgaaccg
     3541 aagatgcgct ccgatttgcc gaagccggtc atgctgtaaa tcatacgagg cgtttgctcc
     3601 caaagcggga ctgatgccag ttccacgctg tttgtattat agtatgtata tcgttgtatt
     3661 gcggttgcca tccgagtatg gcttttgctt tttcagcgct tccgacaagc acggccggat
     3721 ctcctggcct gcgttcgccg tagtgaacgg gtatattttt gcctgttact ttttcacttg
     3781 cggcaataat ctctttgact gaaaaacctt taccgtttcc gaggttaaaa aaatcactac
     3841 agttctcacg cgtcaaaaag tctaacgctc taatatgggc gtcggcaaga tcggtaacgt
     3901 gaatataatc gcgaatgcat gtgccgtcgg gtgtttcata atcggtaccg taaacagtga
     3961 tctgcgatcg gcgacccatc gcagcatcca atacgagcgg aatgaggtgc gtttcgggat
     4021 tgtgatcctc accgataccg ccatccggat cggcgccgga ggcattaaaa taccgaaggc
     4081 acaccgactt caaaccatgc gcccgatcaa agtccgtcaa aatttgctct accatttttt
     4141 tggtttgccc gtagggattg atcggcgcct gagggtgatc ttccgtcaat ggcgtttttt
     4201 gcggtatacc gtacgtcgca caggtggatg aaaaaactaa ttttttaaca tgatgccgca
     4261 ccatcgcttc taaaagatgg agcgttgacc cgacattgtt ttgataatat ttcccgggat
     4321 tttcgaccga ttcgccgacg taagcatacg ctgcaaaatg aagcacggcc tgcggtttgt
     4381 gttgttcaaa agtcttttcg agcatcgcca tatctgatag atcggcctga atcaacggac
     4441 agccctgtgc aaaatccgca tgcccgaaaa tcaggttatc aaaaattacg acatcatgtc
     4501 cggcgcggag taacaggcga acggcatgcg aaccgatgta tccggcgccg ccggtgacga
     4561 ggattttcat gatgtacgct tatctgtagt tttcaaattg catggcaaaa tcaaaattct
     4621 tttctttgag cgttttgatg atagcctgca gatcatcaat gtctttggcc tgtacgcgca
     4681 cctgatcacc ctgaattgag gcttgcactt tcatcttgag atctttgatc attgcgacga
     4741 tttttttggc ctgatcctgt tcgatcccgt tttgcaattt gatatgctga cgtaccgtgc
     4801 tgccggaagc ttcttcgact ttcccatatt tcaaggcttt gatcggcacg gcgcgtttga
     4861 tcattttgtt ttgcaaaata tcaatgaccg ctttgagttt aaaatcatca tcggaatgga
     4921 taacgatttc gtcttttccg agttcgatcg tacttttgct gtctttaaaa tcatagcgcg
     4981 tctgaatttc tttaacggcc tgattgacag cattagtaag ctcttgcata ttgattttgg
     5041 aaacgatatc aaacgaatgc atgtccgcca taaaatctct ccttttattc gcggggcaat
     5101 ttaacataat acggcataaa aacaatatcc aagcggttat gccacgcaca catagatgtc
     5161 gccgtttgtt atttcgatgg tgtgcgcagt gaggtacaca tccggtttac cgaggcacgc
     5221 accgttgcgg atgtcgtatt gccagccgtg ccacttgcag gtcagcgtgt actcttttag
     5281 ctcgctctgc gaaaccaacg atgcgcgcat gtgtttgcat aaaccatcgt aggcgcgcag
     5341 ttgaccgtca atacgataga tcatgatttc tttggctaat accttgatgg aacggccctg
     5401 accttcagcg atttcggatt ctttgcagac tttaaatttt cgcataaatg tatgataagg
     5461 cgagggtatc ggttaaataa tttgaatgac taaacatttg agatattgcg tttcgcgcat
     5521 actcataagt atcggatggt ctttgggctg tgtgcgttct tccaagagca acgcctgtcg
     5581 accggctgca gtgattcctt gaaataccgt attccgaaac gtatcgggat caatcgcttg
     5641 cgaacaactg caagtaaata aatagccgcc ggagggaatc gccttggccg cacgacgatt
     5701 gatttcacgg tatcctttga tggcttcttt gacttgcgat ttagatttgg cgaatgcggg
     5761 cgggtcaagg atgatgacat caaacgattc cctgcgatcg ttgattgctt tgagttcatc
     5821 aaaaacgtca acgcatttaa attggcattg tgtagtttga ttgatttccg catgggtacg
     5881 gcattgggcg atggccgctt cggaaatatc cagtccgata acttccgtcg caccgccgag
     5941 cgcggcatgc acggcccatc cgccaagatg cgtaaagcag tcgagtactc tttttccgga
     6001 tgtaaaacgc cttacataag catgattttc tttttgatcc aaaaaaaatc cggtcttttg
     6061 gccatgcagc agatcgaggc gaaacagcag gttttgaatc gtcacttcga ccatcgaagg
     6121 acaatcacca tacgctacac gcacttcttc aggcaagtgt tcaagttttc gcacagcgga
     6181 gtcattgcgc agaataatcg ctttgggcga aaattcactg cgaatgatat ctacgatcat
     6241 gtcttgatgg atatccatgc ctgcggtgag aatttgcata acccaataat cgccgtaccg
     6301 gtctatagtt aggcccggca gatcatcgga ttcgccgaaa acgacccggt aacaggattg
     6361 gtccgcgtaa aaaacctgtc gccgctcgta agcgttccgg atgcgttcga gaaaaaatgg
     6421 acgattgatt tcaaccggtt taaagctcag gatgcgcacg gcaataagcg aatagggatt
     6481 aatatatccc acaccgacag gataaccgtt ggcatccgtt attttgacgg actcgccggg
     6541 ctcatacgat ttgagcgagt catggatttc gttgctgaat atccaccgat gaccgttgcg
     6601 aatgcggtga tcgtgtttgg gcttgagttg tatagttttc atggcggacg aaaatatatg
     6661 atttgcgtgt gataagcaaa cgtaagaagg tttgccgtga tttaagatcg tatgggtaaa
     6721 tgagatacac ccgttcattc atatgctaag agcggttttt acggcgtact aaaaatacat
     6781 gcgaattatt ttcatgctca agcgctgtat tttgtgatcc gacgaaagat ttaacaatgc
     6841 gattgtggag gtgttaatta cggtaaaact aacgttgaac cgtaacggtt tttttaaaga
     6901 taaaccgagg ttaatttttt gaattttctg attgacaatc cttattgagt tgtatatctt
     6961 cgcccagttt tagaataata ttacacctaa cgttatagga gttttgcaat gcccaatcat
     7021 aagtccgcgg tcgagcgtgt tcgccgaaac aaaaagcagc aggacaaaaa caaaaatcac
     7081 aaacgccaga tgaaagaggc ggtcaaagcg gtcaccacgg c
//
LOCUS       DAOQGF010000148         7029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5219_length_7029_cov_6.823344, whole genome shotgun sequence.
ACCESSION   DAOQGF010000148 DAOQGF010000000
VERSION     DAOQGF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5219_length_7029_cov_6.823344"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1968)
                     /locus_tag="PK937_14280"
     CDS             complement(127..1968)
                     /locus_tag="PK937_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNH33789.1"
                     /translation="MRRLILVLIFICTGIMLLSWLSHSTTTTYGYVVRPTNRQPIANA
                     RVREIGSSDFVLTNASGLFALTTNTSPGDFLNVSAGKEGWYNVRKIMTVGITDTIFLD
                     PLTDEDNSEYTFQDPNFCDHCHTMLHDQWRTSKHARAATNPMFKQMYTGKDALGNANV
                     YPGFKMDFPSQGGDCGDCHAPSAALQSPGNTFMEDVWAQGGVDTNGVHCDFCHKVQSV
                     EVNYKTGVNGSLFVLRPQAHARDINFGPYDDVTTVWMGGTYNPVYEQSAYCSGCHQYA
                     NKFDVIVDDTYDSWAASTYADQGIQCQHCHMRPNSDSTFVNGISSVDAVVRDTNRVFS
                     QFFQGTTEAFLKSAAKLDVDFTLSANRVTLSSRVTNRAAGHKLPTGVSFRNMLLVVEV
                     KDGFENLEFLTGDTLPTYAGIGSSANGNFSGLPGKGYAMLLRDGSGQIPAPNWSATQI
                     IEDTRLPAKTTDTVRSMFRIGRRTSAQVHVRLLYRAVFKAWAEAKSWDMREFLMTDTT
                     FTIDFDKPYQEPERVVLFPNAPNPFNGSTRITYDVPEDNTPVSVTVYNLLGQSIATLY
                     RGKPNAGRHQTHWDGRNSRGVSVASGVYLCRLEAGNTVKTQKILYIK"
     gene            2340..3758
                     /gene="radA"
                     /locus_tag="PK937_14285"
     CDS             2340..3758
                     /gene="radA"
                     /locus_tag="PK937_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNH33790.1"
                     /translation="MAKTKSYFVCQSCGYKSSKSLGRCPECMTWNAMVEEQSATGSSS
                     ARTPDNILKQFGAVDASTYADFSETPQTLDRIEVRPDHRLVSGIAELDRVLGGGIVKG
                     SLVLVGGEPGIGKSTLLLQMAAYQRAKTLYVSGEESVQQIKSRAERIQAPHGGMLVVA
                     ETNLHKILQHILQTKPQVVVIDSIQTLYRDEMESAPGSVGQVRECTAILMHLAKTHGI
                     SIFLVGHVTKDGMIAGPKVLEHIVDTVLQFEGDTNHLFRILRAAKNRFGSTNEIGIFE
                     MRQEGLAEVANPSELFLSERYLHHSGSVVTPVVEGSRVLLVEVQALASVTNYGMPQRT
                     TSGFDLRRLQLLIAVLEKRARIPLGQYDVFLNIAGGLKTDEPAIDLAVALAVASSHRD
                     FVVDPYTAVIGEIGLGGEVRAVSFAERRVREALKLGFETVILPKSNMKDVSFVSGVNL
                     IGIRRIEEVLDAVTAHTGRTES"
     gene            3760..4107
                     /locus_tag="PK937_14290"
     CDS             3760..4107
                     /locus_tag="PK937_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33791.1"
                     /translation="MYLRDWIIALHEKKRFPAHLCLDTGCTLVIEKIKPLVIVLNALV
                     DNLRSVSTQEIHIDLDAHQKKDQLVLIVHTDRETKPIVKTTITDAIHEIGAEFYEEFM
                     PKQYLKYQLTFSR"
     gene            4124..4831
                     /locus_tag="PK937_14295"
     CDS             4124..4831
                     /locus_tag="PK937_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNH33792.1"
                     /translation="MGRIFEKRKYKMFARFDRMAKTFTRIGKEVAIAVKQGGPDPNTN
                     PRLRAAIANAKAANMPKDRVDAAIKRASSKDEKELQEIIYEGYGPHGIGMIVECATDN
                     PTRTVANIRMYFTKHGGTLGSSGSVAFLFERKGVFKIGKEGVDSDELELEMIDYGAED
                     FAVDDEDIYIYTSFGDFVKMQKALEEKKINVKSAELQYIPSTQTDVTEKQEEDVIKLV
                     EAIEEDDDVQNVFHAMK"
     gene            4857..5402
                     /locus_tag="PK937_14300"
     CDS             4857..5402
                     /locus_tag="PK937_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNH33793.1"
                     /translation="MGAILKQEPVKLICGLFWNPEVNPETIYRVIEAEWGPMDARSPV
                     FDFDFTAYYHGEMGAVIQKQYVAFEKLVDIEAIADYKIRSNALEDQHFTRDGKRAANV
                     DPGYIADAKLVMATTKNLPHRVYIGKNIYSDLQMMYKKPTFFPIPWTFADVKQPALIE
                     FFNHVRETYIRQIKTLRQNEV"
     gene            5399..6292
                     /locus_tag="PK937_14305"
     CDS             5399..6292
                     /locus_tag="PK937_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNH33794.1"
                     /translation="MSLALHHVVKSYADTTAVKGLSFEVERGEIFGLIGPNGAGKTTT
                     MRMIIGMIKPDEGWITWLGEPLQASFNDRIGYMPEERGLYRRSRVLDTIVFFAKLKGL
                     SKKTALAEAHRWLEKTELSAYATHRLDALSKGMQQKIQFICTVLHKPELVILDEPFSG
                     LDPVNQNLMRAWIRELADAGMTVIFSAHQMTHVEKLCRRICMMHHGVPVLHDTIAAIR
                     RRHDDGRIRLTGSAEALREVQNTMAWSSLHISDQVLDGIPPAPMSFNQILQRIATLDG
                     IEGIERVHPTLEDIFLKVTKD"
     gene            complement(6540..6950)
                     /locus_tag="PK937_14310"
     CDS             complement(6540..6950)
                     /locus_tag="PK937_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33795.1"
                     /translation="MLLRSMKITIIVALGLGMLKSCSDDSSYESIAGEWEGTVFTGEG
                     DTYIEMKLEQDAENVSGYFRAIDQFSDGGGDSGTIEGTMDGDQFTGHAHGSDCSPDID
                     MTLTHPDTLAGHVHMGACDDGATHALTVYRKPTL"
BASE COUNT         1861 a         1722 c         1759 g         1687 t
ORIGIN      
        1 cgatggcatg gcttgcagag gaaaacgcca aagaccagtt ttgtgcaagc tgagcacata
       61 ccggcgaagc gataacaaaa aataaaacga tgtggcgtaa gcggtacgtc atacggaatc
      121 cttcatttat tttatgtaca aaattttttg tgttttgacg gtatttccgg cttcaagtcg
      181 gcaaagatac acgccggaag caaccgaaac gccgcggcta ttgcgtccat cccaatgtgt
      241 ctgatgacgt cctgcgtttg gttttccccg gtaaagggtg gcgatggatt gtcctaaaag
      301 attatagacc gtcaccgaca caggcgtatt atcttcgggt acatcgtacg tgatgcgtgt
      361 acttccgttg aaaggattag gcgcattggg aaataacact acgcgttccg gttcttgata
      421 tggtttatca aaatcaatcg taaacgtcgt atcggtcatc aaaaattcgc gcatgtccca
      481 tgatttggcc tcagcccatg ctttgaatac agcacgatac aaaaggcgaa catgcacttg
      541 agcggaggtt cttcgaccga tacggaacat cgatcgtaca gtatccgttg tcttggccgg
      601 taaacgtgta tcttcgatga tttgggttgc tgaccaatta ggcgccggta tctgaccgct
      661 accgtcacgt aaaagcatag catatccttt gcccggcaaa cctgagaagt ttccgttggc
      721 cgacgatcct ataccggcat atgtcggcag ggtatcaccg gtgaggaact ccaggttttc
      781 gaagccgtct ttaacttcga cgaccagcaa catatttcga aaactcacac ccgtcggcaa
      841 tttatgaccg gcggcgcgat tggttacgcg tgaggataat gtcacgcgat tggcactgag
      901 tgtaaaatcc acatcaagtt ttgccgcgga ctttagaaaa gcctccgtcg taccctgaaa
      961 aaattggcta aacacccgat tggtatcgcg tactacggca tctacgctgg atatgccgtt
     1021 gacaaacgtc gaatcgctat tggggcgcat gtgacaatgt tggcattgga tgccctgatc
     1081 ggcgtatgta ctggccgccc acgaatcata cgtgtcatct acgatgacat caaatttatt
     1141 ggcgtactga tggcaaccgg aacagtaagc actttgttca tataccggat tgtacgtgcc
     1201 gcccatccat acggtagtca catcatcgta gggaccgaaa ttgatatcgc gcgcgtgtgc
     1261 ttgaggacgc aaaacaaata acgaaccgtt cacaccggtt ttgtaattca cttctacgct
     1321 ttggactttg tgacaaaaat cgcaatggac gccattggta tccacaccgc cttgcgccca
     1381 aacatcttcc ataaatgtat tgcccggcga ctgtaacgcg gccgatggag cgtggcaatc
     1441 gccacagtct ccaccttgcg atggaaaatc cattttaaaa cccggataca cgttggcatt
     1501 gcccagcgcg tctttgccgg tgtacatttg tttgaacatc ggattggtgg cggcgcgggc
     1561 gtgtttggac gtgcgccact gatcgtgcaa catcgtatgg caatgatcac agaaattagg
     1621 atcctgaaac gtgtattccg aattatcttc atcggtcagg ggatcaagga atatcgtatc
     1681 cgttataccg acggtcatga tttttcgaac attgtaccaa ccctctttac ctgcggacac
     1741 attgagaaaa tcgcccggac tggtattggt tgttaaggcg aaaagtccgg atgcattggt
     1801 cagaacaaag tccgatgatc cgatctcacg tacgcgggca ttggcaatag gctgacgatt
     1861 ggtaggtcgt acgacataac cgtaagtcgt agtggtgcta tgagataacc aagacagcag
     1921 catgatgcct gtacaaataa aaattaaaac gagaatcaat cttcgcatac gatttagaat
     1981 ccctaagata gaactggtac taggagccat taaaggtaca tgtctgtcat ctttagcgca
     2041 atattatttt gaatgggttt tttagtttag agtttataga ctaaaaagta gcaggattaa
     2101 tataaaaaag cggtgtctcg cgtgagggat gcgcagggta cgcggcgccg ccggcgcgcg
     2161 tagcccacgc aaagcgccaa atgcgctgcg gaggccgaga gcccgaagcc agcccgacct
     2221 gacacgccac aggcgtgaag gggcacgccc aagtgatcgg ccgtcgtatc ggtgtgtttt
     2281 tagtagaaat tctcgtacta tcgtattaga ttgcgccgca tttgtaaaac agcggtgtca
     2341 tggctaaaac caaatcttat tttgtctgcc aatcgtgcgg ctacaagtcc agcaaatcgc
     2401 tcggacgttg cccggagtgt atgacgtgga atgcgatggt cgaagaacaa tcggccacag
     2461 gatcatcctc ggcacgcacg ccggataata tactcaagca gtttggtgcg gtggacgctt
     2521 cgacgtatgc ggatttttcg gaaacgccgc aaacgctgga tcgcatcgaa gtgcgaccgg
     2581 atcaccgctt agtctccggt atcgccgagc tggatcgtgt gctgggtggc ggcatcgtca
     2641 aagggtcgct tgttttggtc ggcggcgaac cgggtatcgg caagtccacg ttgcttttgc
     2701 agatggcggc ctatcaacgc gccaaaacac tgtacgtctc cggcgaagaa tccgtgcagc
     2761 aaatcaagtc gcgtgccgaa cgcattcaag ctccgcacgg cggtatgctg gtcgtcgcgg
     2821 aaaccaatct ccataaaatc ctgcagcata ttttacaaac caaaccgcag gtcgtcgtga
     2881 ttgactccat ccagacgctg taccgcgacg aaatggaaag cgcgccgggc agtgtcgggc
     2941 aagtgcgcga atgtacggcc atcctgatgc atctggccaa aacgcacggc atctcgatat
     3001 ttctcgtcgg ccacgtgacc aaagacggca tgatcgccgg tcctaaagtt ttggaacata
     3061 tcgtagatac cgtactgcag tttgaaggcg ataccaatca tctttttcgc attttgcgcg
     3121 ccgcaaaaaa tcgttttggt tcaaccaacg aaatcggaat ttttgaaatg cggcaggaag
     3181 gtttggccga agtagccaac ccatcggaat tatttttatc ggaacgctac ctccatcatt
     3241 ccggttccgt cgtaacgccg gtcgtcgaag gttcgcgcgt gcttttggtc gaagtgcagg
     3301 cgctggccag cgtaaccaac tatggcatgc cgcagcgcac gacgtcgggc tttgatctac
     3361 gccgcctgca actgctgatt gccgttctcg aaaagcgcgc acgtataccg ctcgggcaat
     3421 acgatgtttt tttgaatatc gcgggcggat taaaaacgga tgaaccggca attgatcttg
     3481 ctgtggccct ggccgtcgca tcaagccacc gcgacttcgt cgtggatccg tatacggccg
     3541 tcatcggtga aatcgggctt ggcggcgaag tacgcgccgt cagttttgcc gaacgacggg
     3601 tgcgggaagc gctcaaactg gggtttgaaa ccgtgatcct gccgaaaagt aatatgaaag
     3661 acgtgagttt tgtaagcggt gtcaacctga tcggcattcg ccgcatcgaa gaagttttag
     3721 acgccgtcac cgcacatacc ggacgcacgg agtcttgaga tgtatctgcg ggattggatc
     3781 atcgctttgc atgaaaaaaa acgatttccg gcgcatcttt gtttggatac aggatgcacg
     3841 ctggtcatcg aaaaaatcaa accgctggtg atcgtgctga atgcgctggt ggataatctg
     3901 cgttcggtga gcacacagga aatacacatt gatctggatg cgcatcaaaa gaaggatcaa
     3961 ctcgtattga tcgtacatac cgatcgggaa accaaaccga tcgtgaagac aacgatcacc
     4021 gatgccatcc atgaaatcgg cgccgagttt tatgaagaat tcatgccgaa gcaatatttg
     4081 aagtatcaat tgacattttc acgataaata aaggacgaag accatgggtc gtatttttga
     4141 aaaacgtaaa tacaaaatgt ttgcccgttt tgaccggatg gcaaaaacgt ttacccgtat
     4201 cggtaaagaa gtggccattg ccgtcaaaca aggtggcccc gatcccaata ccaatccccg
     4261 tttacgtgcg gctatcgcca atgccaaagc cgccaatatg cccaaagacc gcgtggatgc
     4321 cgcgatcaaa cgcgcttcat ccaaagatga aaaagaattg caggaaatca tctatgaagg
     4381 atacggcccg cacggtatcg gcatgatcgt cgaatgcgcc acggataatc ctacgcgtac
     4441 ggtggccaat attcgcatgt actttaccaa acacggcggc acgctcggtt cttccggttc
     4501 ggttgcgttt cttttcgagc gtaaaggcgt ttttaaaatc gggaaagaag gcgtggattc
     4561 cgacgaactg gagctggaaa tgatagacta cggcgcggaa gactttgccg tggatgatga
     4621 agatatctat atctatacgt ccttcggcga cttcgtcaaa atgcaaaaag cgctcgaaga
     4681 gaaaaaaatc aacgtcaaat cggcggaatt gcagtatatt ccatccacgc aaacggatgt
     4741 caccgaaaaa caagaagaag acgtcatcaa attagtcgaa gccatcgaag aagatgacga
     4801 tgtacaaaat gtttttcacg ccatgaaata accgcacgga acgggtttta aaaaaaatgg
     4861 gtgcgatcct caaacaagaa ccggtcaaac tgatctgcgg attattttgg aatccggagg
     4921 tcaacccgga aacgatctac cgcgtgatcg aagcggagtg gggtcctatg gatgcgcgtt
     4981 cgccggtttt tgattttgat tttacggcgt actatcatgg cgagatgggc gcggtgatcc
     5041 aaaagcagta cgtagcattt gaaaaactcg tggatatcga agctatagcc gattataaaa
     5101 ttcgctccaa tgcgcttgaa gatcagcact ttacccggga cggaaaacgt gcggccaatg
     5161 tagatcccgg ttatatcgcc gatgccaaac tcgtcatggc gacaactaaa aatctgccgc
     5221 accgcgtgta tatcggaaaa aatatttatt ccgacctgca gatgatgtat aaaaaaccga
     5281 ctttttttcc tatcccttgg acttttgccg acgtcaaaca accggcgttg atcgaatttt
     5341 ttaatcacgt gcgcgaaacg tatatccggc aaatcaaaac cctacgccaa aacgaagtat
     5401 gagcctcgcc ctgcatcatg tcgtcaaatc ctacgcggat acaacggcgg tcaaaggatt
     5461 gtctttcgaa gtggagcgcg gtgagatatt tggcctgatc gggcccaatg gcgcgggtaa
     5521 aaccacgacc atgcgtatga tcatcggcat gatcaaaccc gacgaaggct ggatcacatg
     5581 gctcggcgaa cctttgcagg cgtcgtttaa tgatcgcatc ggatatatgc cggaagagcg
     5641 cgggctctat cgccgcagcc gcgtgctgga tacgatcgtt ttttttgcca aactcaaagg
     5701 actgtctaaa aaaacagcgt tggccgaagc gcatcgctgg cttgaaaaaa cagaactctc
     5761 cgcgtacgcc acacatcgtc tcgatgcact ctccaaaggc atgcaacaaa aaatacagtt
     5821 tatctgtacc gtgcttcaca aaccggaact tgtgatttta gatgaaccgt tttccggact
     5881 cgatcccgtc aatcaaaatc tcatgcgcgc atggatacgc gaactggcgg acgccggtat
     5941 gaccgtgatt ttttcagcgc atcagatgac acatgtcgaa aagctctgtc gtcgtatctg
     6001 tatgatgcat cacggcgtac ctgtacttca cgacacgatc gcagcgatac gacgtcggca
     6061 cgatgacgga cgcatacgcc tgaccggttc cgcagaagcc ctgcgcgaag tgcagaacac
     6121 gatggcatgg tcatctctgc acatttcaga tcaggtatta gacggcattc cgccggcccc
     6181 catgtcgttt aatcagatat tacaacgtat tgcaacactc gacggcatcg aaggtatcga
     6241 acgcgttcat ccaacgttgg aagatatttt tttgaaagtg acgaaggact agagcgaagg
     6301 aagggtacat cggaaggcat ccgctctgga ttcacagagt ttcattgttt gaatgaaaaa
     6361 atcactacct cgtccgatta ataataacgc ggactggctg attgataact acgaaaaaaa
     6421 ataaactttc tcctgcgtac agcatcctct taacggctag attcctctcg ttatgaaagg
     6481 ccgtaagcgc ggtgatcacg ttctcaactt atttgatttc tcccaaaccc tttactgcgt
     6541 tataaagtcg gctttctgta aaccgtgagc gcgtgagtcg cgccgtcgtc gcatgcaccc
     6601 atgtggacat gaccggctaa cgtatcggga tgggtgagcg tcatatcaat atccggtgaa
     6661 caatcggaac catgtgcatg cccggtaaac tgatcaccat ccatcgtacc ttcgatcgta
     6721 ccgctgtccc cgccgccgtc actgaactga tcaatcgcac gaaaatagcc cgatacgttt
     6781 tcagcatctt gttcgagctt catctcgatg tatgtatcgc cttcgccggt aaatacggtt
     6841 ccctcccatt cgccggctat actttcgtat gaactgtcgt ccgagcagga tttgagcatt
     6901 cctaatccca gagctacgat gatggttatc ttcatggatc tgagtagcat aaaaaacctc
     6961 tcaacgtgat ttgagccgta gcatgattgc taatgctctc atgatcaatg tacgaaatag
     7021 gcgtagctt
//
LOCUS       DAOQGF010000149         7022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5229_length_7022_cov_6.585331, whole genome shotgun sequence.
ACCESSION   DAOQGF010000149 DAOQGF010000000
VERSION     DAOQGF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5229_length_7022_cov_6.585331"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PK937_14315"
     CDS             complement(<1..1029)
                     /locus_tag="PK937_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNH33796.1"
                     /translation="MVRIGLFCFLFHSAVAQNDIMLLYRDAMALMVKKNFEHAEQKLR
                     ILDSLMPDDTEIYFLRAQVLAQMGDHTKAMGLLKASLIMGSDMLFAIGKDSAFVGLWA
                     DSEFQNLLKEMEQLKRPVHHSRDAYLLTERDLIPEGTAYDGHTGQLFISSTYKGKIKR
                     IYPDGRSDDFTKEGQDGLLGVVGMEVDSVRRILWVNSCNNPNMPIRDSHADARVPARV
                     HKFDLTTGRLLQRYDAPAGSHFFNDLTVTAKGDVFITDTESGEIWQIAAERHTLEIFL
                     KLQNVLFLNGITKSPDDQWLYVAHVAGILVIDIATRRYEPLKHPPLFPLGSIDGLAFY
                     KQSLIAHQS"
     gene            complement(1040..1504)
                     /locus_tag="PK937_14320"
     CDS             complement(1040..1504)
                     /locus_tag="PK937_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33797.1"
                     /translation="MKTLLMIAFLVPCIGCRVNPDLEAEKTELLRLHEAQRRAHFDRN
                     VDPMTEGHVPQRIQISGGELSYTTPEQTRARFERYFGRVEFLRWDDTIEPIIVVSDDA
                     TLANTFVQKLVVVSYQDERGAKQLDTTRFAWTTTYRKINDQWQMIAVTSTRK"
     gene            complement(1557..2489)
                     /locus_tag="PK937_14325"
     CDS             complement(1557..2489)
                     /locus_tag="PK937_14325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNH33798.1"
                     /translation="MNYFDIIYTEVLTYLGFFVVNPIACAYVAMLQTALLMSAGLIAL
                     GIYKQNRTIKMWKKNSIQPDKRWMATVEKINRRIGLSSLPGLRFTHQALPICTVGFFK
                     PVIYIPEPMVRNLTDSDLAMIAKHELWHIKKRHNLKKLIMRSLGLVGSLWSGFATGLA
                     LTYRVLDQRYEIIGLVLSFILGLILMRTFRRYVIPYFDLWCEYECDDALQMCRNQRQT
                     MAAMLVRMGKMNIISDPMRDMIRCAAVSHLEKRVRRLISEPASNAFTPYIRGVLYFVA
                     IVLIVNVINIHRAHNFQWVSGHGKRMHLCAGQCK"
     gene            complement(2486..2905)
                     /locus_tag="PK937_14330"
     CDS             complement(2486..2905)
                     /locus_tag="PK937_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNH33799.1"
                     /translation="MSKHTFTFDPRGKGLRKVLGDLESDIMEVVWEYESVTVRDVCTH
                     LNKKRPLAYTTVMTVMTRLAEKGVLRKIKSGAAFVYQPARSRESFTLSASVQIFKSLF
                     DDFSKPVMSELIDTIHRENPERLDELAALIEKKKKKK"
     gene            complement(2947..3537)
                     /locus_tag="PK937_14335"
     CDS             complement(2947..3537)
                     /locus_tag="PK937_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH33800.1"
                     /translation="MKIPKLGSDIPTFGNALSRKIGYMILRSMRWRIEGEIPNLKKMV
                     IIGAPHTTNWDFVIGMSALMALGVRVHWIGKDTIFKWPVKYVWHWLGGEPVDRFHAHG
                     VVEQIVQKFSAHDQFILGLSPEGTRRDIPKWRSGFYHVSVGAGVPILLVAFDFPSKTL
                     RIGELFYPTSNPDSDMLQLTGYFKNFRGKFVKTWQK"
     gene            complement(3687..4415)
                     /gene="truA"
                     /locus_tag="PK937_14340"
     CDS             complement(3687..4415)
                     /gene="truA"
                     /locus_tag="PK937_14340"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNH33801.1"
                     /translation="MRNIRIVVEYDGTDFHGWQYQSHCRSVQGTLQEAVHRITSENIV
                     VEGAGRTDTGVHARGQVANFKINHAIAEGRIALALNAVLPTDVRVISAQEAPDTFHAR
                     FSARERRYRYRITPIPAALQRHYCWFYPHAMNWEAMQVACGELIGEKNFKSFCLSETV
                     LPHYQCDVKSAVWLMENDVHVFEIRANRFLHNMVRGIVGTMIMIGRGRLMPGDIATIL
                     AKAHRRHAGFNAPPHGLCLEEVIY"
     gene            complement(4580..6760)
                     /locus_tag="PK937_14345"
     CDS             complement(4580..6760)
                     /locus_tag="PK937_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH33802.1"
                     /translation="MKKWILLCMTGIGLSVPQLLNAQHALTVQVTDSESGDAVPGAQV
                     HIMGTTQGGVSNAEGIVRLENIAEGIYRLRITSAGYEDVIMEIQLPRTDANPIPIVLE
                     HASHEGEEITVTSTRSSRTIENTPTRVEAIAAEELDEKANMRVSDIRMQLNESTGIQV
                     QQTSVTSGNAVFRIQGLDGRYTQLLKDGFPLYAGFSGGLSITQIPPLDLQQIEIIKGS
                     ASTLFGGGAIAGLINLVSKTPQEKPETLLMVNGTTAKGFDGNIYHARRNEKTGYSVFS
                     SAHTQSPYDADEDGFSDIPKIHRFALNPRVYYYPGERSTLMLGFDGVLERRNGGDMQR
                     LENGKDSMHTYIEKNRSRRLSTQMMWQQPLFGRATMTFKNSFSFFDRAIVLQDYRFTG
                     YQQSSFSEWTLGYTHKNNSWIMGINLWTDRFDEQKSSLKRDFHSLTAGVFVQHQAEWS
                     EDWSSEAGLRYDRDKDYGSFVLPRLSVMRRWNASWTSRLGGGLGYKSPTIFNETSETQ
                     SYRNIRGIQKNQLKAETSLGGNFDINYQNVWAKRFPVALNTMVYYTRLRHALTYDKDS
                     LASGVYDLINSDGHIDTRGTEINGKISYEAWKLFVGYTWVDIKRHENGGTSHIPLSAK
                     HRLGLVLVYEEEARWRAGIESYYTGHQYRSDGRSTNDFWIFGFMIQRTMGPHAVFVNF
                     ENFTDTRQSRFEALYTGTRTNPDFKELYAPTEGFVANAGLKIRW"
BASE COUNT         1884 a         1758 c         1509 g         1871 t
ORIGIN      
        1 cgactgatgc gcgatgagtg attgcttgta aaaagccagc ccgtcaatgc ttccaagcgg
       61 aaatagcggc ggatgtttca aaggctcata ccggcgtgta gcaatatcta taactaaaat
      121 tcccgccaca tgggcaacat aaagccattg atcgtccggc gacttggtaa tgccattgag
      181 aaaaagcacg ttctgcagct tcaaaaatat ttctagcgta tggcgttcag cggcgatctg
      241 ccagatttct cccgattcgg tatcggtgat aaaaacatcc cctttcgccg tgacggtaag
      301 atcgttaaaa aaatgtgaac ccgccggagc atcataccgc tgcagcagac gtcctgtcgt
      361 aaggtcaaat ttatgcaccc gcgcaggaac ccgtgcatcg gcatggctat cccgtatcgg
      421 catattcgga ttattacagc tattaaccca taaaattcgc cgcacggaat ccacctccat
      481 cccgactacg ccgagcaagc cgtcttggcc ttccttagta aaatcatcgc tgcgcccgtc
      541 cggataaatg cgtttgattt ttcctttata ggtgctgctg ataaaaagtt gccccgtatg
      601 tccatcgtag gctgtgccct cgggtatcaa atcccgctct gtaagaagat aagcatcacg
      661 gctgtgatgg acgggccgtt taagctgttc catttctttg agtaaatttt gaaactcgga
      721 gtcggcccac aaacccacaa atgcgctgtc ttttccgatt gcgaaaagca tatcgctgcc
      781 catgattaga ctcgccttca ataggcccat tgctttggta tgatcaccca tctgtgctaa
      841 aacctgcgct cggagaaaat aaatttccgt gtcatccggc ataagactgt ccagtattcg
      901 gagtttctgt tccgcatgtt caaaattttt tttcaccatc aatgccatcg catcacgata
      961 caaaagcata atatcatttt gagcaaccgc ggaatgaaac aaaaaacaaa acaatccgat
     1021 tcgtaccata ttcatcccct tattttcggg tggaagtcac agcgatcatc tgccattgat
     1081 cgtttatttt tcggtaagtc gttgtccagg caaaccgggt tgtatccaat tgtttggcgc
     1141 cgcgctcatc ctgataagat accacaacca gtttctgaac aaatgtattg gccaacgtag
     1201 catcatcaga aaccacaatg atcggttcga tcgtatcatc ccaacgaaga aattcgacac
     1261 ggccaaaata acgctcaaac cgggcacgcg tttgctccgg cgtggtgtag gacaattctc
     1321 ctccgctgat ttgaatacgt tgcggtacat gcccttctgt catcggatct acgttgcgat
     1381 caaaatgggc acgccgttgt gcttcgtgta agcgtaacag ctccgttttt tccgcttcca
     1441 aatcgggatt gacccgacac ccgatgcatg gcacgagaaa cgcgatcatg agtagtgttt
     1501 tcatgaatct cctttttatt ttctactaca tagtgtagta ttttatggta aaaaaattat
     1561 ttacattgcc ccgcgcacaa atgcattcgt ttgccgtggc cggaaaccca ttggaaatta
     1621 tgtgcgcggt ggatgttgat cacgttgacg atcaatacta tcgctacgaa gtatagaaca
     1681 ccgcgaatat aaggcgtaaa cgcgtttgat gcgggttcac tgattaaccg tcgaacgcgc
     1741 ttctccaaat gcgaaaccgc tgcacatcgg atcatgtccc gcatcggatc ggaaataata
     1801 ttcatttttc ccattcgaac cagcattgcc gccatagttt gtcgctgatt acgacacatc
     1861 tgcaaagcat cgtcgcattc atattcacac cacaaatcga aatacggtat aacataacga
     1921 cggaatgtgc gcatcaaaat tagacctaat ataaacgaaa gcactaatcc gatgatttca
     1981 taccgttgat ccaaaacacg ataggtcagt gcgagtccgg ttgcaaaacc cgaccaaagc
     2041 gaacccacga gccccaatga acgcatgatt aattttttca gattgtggcg tttttttata
     2101 tgccacagtt catgttttgc aatcatcgcg aggtcggaat ctgttaaatt tcgcaccatc
     2161 ggctccggta tataaataac cggtttaaaa aaacccaccg tacaaatagg aagcgcctga
     2221 tgggtaaaac gtaatcccgg tagcgaagag agcccgatac ggcgattgat tttttcaaca
     2281 gtggccatcc atcgtttatc gggttgtatt gaattttttt tccacatttt tatggtgcgg
     2341 ttttgcttat aaatacccag tgcaataaga cccgcgctca tcaagagtgc cgtttgcaac
     2401 attgcgacgt acgcgcaagc aatcggattc acaacaaaaa acccgagata agtcaatacc
     2461 tccgtataaa taatatcgaa gtaattcatt tctttttttt ctttttctct atcaatgcgg
     2521 ccaactcgtc caatcgttcg gggttttccc gatgaatcgt atcaatcagt tcgctcatta
     2581 ccggtttaga aaaatcatca aaaaggcttt taaaaatttg aaccgatgcg cttaacgtga
     2641 acgactcccg tgatcgtgcc ggttggtaaa caaacgcggc gccggatttg atttttcgca
     2701 atacgccttt ttctgccaac cgcgtcataa ccgtcatcac ggtagtataa gccaaaggtc
     2761 gctttttgtt gaggtgtgtg catacatcac gcacggtgac cgattcatat tcccatacca
     2821 cctccatgat atcggactcc agatcgccaa gcactttgcg taaaccttta ccgcgcggat
     2881 caaatgtaaa tgtatgttta gacataatcc tactacgtta tgtagttttt aaagtttcaa
     2941 aaaaaattac ttctgccacg ttttaacaaa ttttccgcgg aagttcttaa agtatccggt
     3001 taactgcagc atatccgaat caggatttga cgtggggtaa aacaattcgc cgatacgcag
     3061 tgttttcgac ggaaaatcaa atgcgactaa taatatgggt acaccggcac cgacggacac
     3121 atgataaaaa ccgctgcgcc attttggaat atctcttcgc gtaccttcgg gcgacagccc
     3181 gagaataaac tgatcgtgcg cgctaaactt ttgaacgatt tgttctacca cgccgtgtgc
     3241 gtgaaatcgg tccaccggtt ccccgccaag ccagtgccag acatatttta caggccactt
     3301 gaaaatcgta tcttttccga tccagtgcac gcgaacaccg agcgccatta aagccgacat
     3361 cccgattaca aaatcccaat tcgtcgtatg cggcgcaccg ataattacca tctttttcag
     3421 gttaggtatt tcgccctcaa tacgccaacg catggatcgt aaaatcatat aaccgatttt
     3481 acgggataga gcgttaccaa atgtaggtat gtcgctgccg agtttaggaa ttttcatagc
     3541 acaacaaagt atgttcgccc tcaatgtacg cgcataaatt acttagctgc aaaggaaatt
     3601 attatgaagt ttacctacga ataggacgcg ctgtcatata gaaatgaagt agttttgtgt
     3661 ggcggcaatg atatgatttt tccgcgtcag taaatcactt cctctaagca caaaccgtgc
     3721 ggcggcgcat taaatcccgc gtggcggcga tgtgctttag ctaagatcgt tgcgatatca
     3781 cccggcataa gacggccacg accgatcatg atcatggttc cgacgatacc ccgtaccata
     3841 ttatgcaaaa aacgattcgc ccgtatttca aacacgtgga catcattttc catcagccat
     3901 accgccgact tgacatcgca ttgataatgc ggcagcacgg tttctgacaa acaaaacgat
     3961 ttgaaatttt tttcaccgat caattcccca cacgcaactt gcatagcctc ccaattcatt
     4021 gcatggggat aaaaccaaca ataatgacgt tgtaatgcgg caggtatcgg tgtgatacga
     4081 taacgataac gacgttcccg cgccgaaaaa cgcgcatgaa atgtatccgg cgcttcctgt
     4141 gccgaaatga cgcgcacatc cgtcggcagg acggcgttta gtgccaacgc gattcttcct
     4201 tcagcgatag cgtgatttat tttaaaattg gccacctgtc cgcgtgcatg cacacccgta
     4261 tcggtacgcc ccgcaccttc gacgacgatg ttttcggatg tgatgcgatg tacggcttcc
     4321 tgcagcgtac cttgtacgga gcgacagtgc gattgatatt gccatccgtg aaaatccgtg
     4381 ccgtcgtatt ctacgactat acgtatgttg cgcataccgt gattgtatta ctgattaaaa
     4441 ataaacgacg tatactatga aatatactcg ttgacgtttt atgaagtatt taaaaaaaat
     4501 atcccgccgt tttattcggc gggattatag tacaaacgaa caactacaaa atcaaaaacc
     4561 cgtattcggt acgtgatgct taccagcgta tttttaaacc tgcattcgcc acaaaaccct
     4621 ccgtcggcgc atagagttcc ttaaaatccg gattggttcg cgtaccggtg tacagcgctt
     4681 caaagcggga ttgacgcgta tccgtgaagt tttcaaaatt gacaaatacg gcatgcgggc
     4741 ccatggtgcg ctgaatcata aaaccaaaaa tccaaaaatc atttgtagaa cgaccatcac
     4801 tgcggtactg atgaccggta taatacgatt cgatacccgc acgccaacgt gcctcttctt
     4861 catacacgag tacaagccca agccggtgtt tagccgatag cggtatatgc gatgtaccgc
     4921 cgttttcatg acgctttata tccacccatg tataaccgac aaataatttc caggcttcgt
     4981 aactgatttt tccgttgatc tccgtaccgc gtgtatcaat atgcccgtcg gaattgatca
     5041 aatcgtacac gcccgacgcc agcgaatctt tgtcatacgt cagcgcgtgc cgcaaccgcg
     5101 tataatacac catggtattg agcgccacag gaaatcgttt tgcccaaacg ttttgataat
     5161 tgatatcaaa atttccgcca agcgatgtct cggctttaag ttgatttttt tgaataccgc
     5221 gaatgttgcg ataggactgc gtttccgacg tttcattgaa tatcgtcggc gatttataac
     5281 caagtcctcc gccgagccgc gatgtccacg aagcattcca gcggcgcatt acggacaaac
     5341 gaggtagtac aaacgaaccg tagtctttat cgcgatcata tcgcagaccg gcttcggatg
     5401 accaatcttc actccactcc gcctgatgtt gaacaaatac ccccgctgtc aggctatgaa
     5461 aatcacgttt tagggatgac ttctgttcat caaaacgatc cgtccacagg ttgatgccca
     5521 tgatccagga gttattttta tgcgtatatc ccaacgtcca ttcggaaaaa gaagattgtt
     5581 ggtatccggt aaaacgataa tcctgcagca caatcgcacg atcaaaaaaa ctgaagctgt
     5641 tcttaaatgt catcgtggct cgcccgaaaa gaggttgttg ccacatcatc tgtgtggaca
     5701 ggcgccgtga acggtttttt tcgatatacg tgtgcatcga atcttttccg ttctcgaggc
     5761 gctgcatgtc gccgccgttc cttctttcca aaacgccgtc aaatcccagc atcagtgtgc
     5821 ttcgttcacc cggataataa taaacccggg gattcaacgc gaagcgatga attttaggaa
     5881 tatctgaaaa cccatcctca tcggcatcgt acgggctttg ggtatgcgct gatgaaaaca
     5941 cactataccc tgttttttca tttcgccgcg cgtgatagat attgccgtca aaacccttag
     6001 ccgtcgtacc gttgaccatc agtaaagtct ccggtttttc ctgcggcgtt ttgcttacca
     6061 agttgatcag ccctgcaata gccccaccgc cgaatagcgt agatgcactg cctttgatga
     6121 tctcgatttg ctgaagatca agcggcggta tttgcgtgat gctgagcccg ccggaaaaac
     6181 cggcgtacag cgggaaaccg tctttgagca attgtgtgta acggccatca agaccctgga
     6241 tacgaaacac cgcattaccg gaggtaactg acgtttgctg aacctgaata cctgtactct
     6301 cattgagctg catgcgaata tcactcacgc gcatattggc cttttcgtcc aattcctccg
     6361 cagcgatggc ttcgacgcgc gtcggtgtat tttcgatcgt tcgactcgaa cgcgtggacg
     6421 taacggtgat ttcttcgcct tcatgcgagg catgttccag aacaataggg atcgggttcg
     6481 cgtccgtgcg cggtaactga atctccataa tcacgtcctc ataacctgcg gaagtgatgc
     6541 gcagccggta aatgccctcg gcgatatttt caagtcgtac aatgccttcc gcattcgaaa
     6601 cgccgccttg ggttgtaccc ataatatgca cttgtgcacc gggtactgcg tcgcccgatt
     6661 cggaatcggt tacttgcact gtgagtgcat gttgtgcatt taacagttgc ggtacggaca
     6721 gaccgatccc tgtcatacat aataaaatcc attttttcat atcatctccg ttggtgtaaa
     6781 taaacgttaa aaacgtcatc cacgcggcgg ttgaaaaatt ctgctgttat atagggaaat
     6841 gggtacgtcc gtagaagccg gccggtatgt cgtaaaataa aaatgaagcg gcattttttt
     6901 gatataaaaa caaatacaat aatgcgaaca gcacgcacaa ccgtccatcg gcgaacacct
     6961 atcggcgcag gattcttcgt ggttttcatc atgcacccgg atcgtcatgc ggttatcggt
     7021 at
//
LOCUS       DAOQGF010000150         7017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5234_length_7017_cov_4.485349, whole genome shotgun sequence.
ACCESSION   DAOQGF010000150 DAOQGF010000000
VERSION     DAOQGF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_7017_cov_4.485349"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..498
                     /locus_tag="PK937_14350"
     CDS             172..498
                     /locus_tag="PK937_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33803.1"
                     /translation="MLAAALLSQIKIKMRSYKNELEAIANDLLTQNAEAKGNENKPNY
                     SNRDFMNATIIFQTALMDKMYDNQDYDGMSVDDRMKMAESCGLALRKLIHTYTGLDTH
                     KLEEFL"
     gene            611..1486
                     /locus_tag="PK937_14355"
     CDS             611..1486
                     /locus_tag="PK937_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33804.1"
                     /translation="MPLQMMRRLDPNFEFEITEKFSDGDLTRFDALLLFRPSYPVEMS
                     AIATAKKMGLKVIVDYDDDFQNVPEFSPVFGKIMEPERQNVVRAVAATADMVWTTTEA
                     ISTAMQRPDAHVIPNAVPEIWIPKKPAKNTKSASWRGREMQYMDVVFQGIVSGWYNQI
                     ATLPEVWTWMGWMPFPRAEGPKHVFREYGNITQYMDYVRNAGINVLWKPLVECQFNDA
                     KSNISWIEATIGGGVCVTNYAGKKWWEHALPDFTFDDKVLRETWEVSAQSVYERHNLK
                     ATARQRLTLLKQLMK"
     gene            1790..3166
                     /locus_tag="PK937_14360"
     CDS             1790..3166
                     /locus_tag="PK937_14360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNH33805.1"
                     /translation="MRIFGYEITFSRKEKRTSDFTGGNYLISNASFSAPYGTKATDQT
                     ILGLSPVWAAIRYISEGVAMLPFDVYRSTPDGNERNPNHPLQYLISERPHPHYSRIDF
                     LSALVANACLGDGFARIHFDPNQRPYALELLPRAIVSMRYGSQGQLLYDIMGSPSPSD
                     VYSPPVIVATLQDYEIIHIKGVTLSGVKGERLTLTHKDGFGAALSAQKYVGNFFDNGA
                     APSGALVYPSLLTKEQIDKAEKKVQNKYSGADNAGRVMILDGGMKFERFSMGPQEAAL
                     VDFRNLSVEDCSRIFKVPLHMLSSLDRSTFSNIEQQEGDYYTHCLPVWSQKIEQEFNY
                     KLFTALERSKRRAFVAFDYTFARMGDSDSTAKMIAATVQNGVMTANEWRKRLNLQTIP
                     DGNRTYIQQNMMPTDMVDEVLENKYEKDKGEAAQDQAPDVEETDTPDAGTDNQNITNG
                     NDTEQPND"
     gene            3135..3737
                     /locus_tag="PK937_14365"
     CDS             3135..3737
                     /locus_tag="PK937_14365"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HNH33806.1"
                     /translation="MATILSNPTIERRYFDSNFEVRKMDDGKHRVCGYALRFGVSYDM
                     GWFTEEISRTALDNADMTDVRILFNHDPNQILGRTSAGTATVRVDDNGLWYEAELPNS
                     PNGENVRVALERGDVTQSSWGFMLRYDDNTQGDLWQRKNGKDHRTITDVSAVFDASPV
                     TFPANPETSAAKRSYDQVLKHEQKLKSKTAEIDCILALHQ"
     gene            3756..5090
                     /locus_tag="PK937_14370"
     CDS             3756..5090
                     /locus_tag="PK937_14370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNH33807.1"
                     /translation="MKNLLEAKQARTDATQRITELRAKATAGTWGEAEQSALDAAKRD
                     LDAADAAVKRFEDFEALETRAIAWTPTATASNPVTVNVISSENRGDKPENVAKQFRLL
                     DAVKESAYGRGLTGVNAEIDQEGKKEARAAGIDKFGTGSITLPSFMVHGEKRDMLAGT
                     TTAGGYTVQTEVGQLIDFLYPRLTVKEMGATYLTGLTGNIDFPRNDAAAAVARTTEGA
                     TSTETSPTFDRVQLTPVRYAAYTDVSKQVAIQSTIGMENFVRNALNSALFRALETEIF
                     TNSGSNGLFDISGTNDITLGTNGGDLTWADVVKFETEVSADNADMGRMGYLFTPQVAG
                     KLKAAKRDVAGNGFIWEGPNMEAQVNGYRAKASNLLPKTLSKGGFTSVLHGGLFGNWE
                     ELIIGQWGGVDLLINPYTKGKEALIEVILNAWFGFAVRHAASFTKCDELYPS"
     gene            5090..5302
                     /locus_tag="PK937_14375"
     CDS             5090..5302
                     /locus_tag="PK937_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33808.1"
                     /translation="MVPVLWHSSGVRFGYAYNFGETGTVKESDLPRLLAAKAVAVIEQ
                     AARVPDKYQAPESAHHQARKYERRKK"
     gene            5299..5868
                     /locus_tag="PK937_14380"
     CDS             5299..5868
                     /locus_tag="PK937_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HNH33809.1"
                     /translation="MTYTVTSGPSSEPLTTSEVKTWLRVTSSTDDTLIESLIKAARMY
                     AENFTGRALFTQTIKEYFDGWPYCGCVELGFGPVQSITSVKYYDTDGTLQTVSSSNYI
                     TDLISLPGRIVNHPDYAWPELQTRTNPVEIIYVAGYTATASIPDGIKAAMNLLIGFWY
                     ENREDIPVNNHNPSVRSADILLHQYKIHL"
     gene            5920..6225
                     /locus_tag="PK937_14385"
     CDS             5920..6225
                     /locus_tag="PK937_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HNH33810.1"
                     /translation="MDDEIVIQRATLAANDYGDPVPTWSTLVSTWAKVEWLENSSDEV
                     YRAGIENEYNRVDFTIRYNSDVRSKDRISFGGNYFDIQTVHYIGRQQFTKLQTVLRQ"
     gene            6222..6665
                     /locus_tag="PK937_14390"
     CDS             6222..6665
                     /locus_tag="PK937_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HNH33811.1"
                     /translation="MKGNGLILTQFALDADLLVKKLERISADFRKDSREIMSKASEPL
                     IVDIKRRAPVKTGRLKIAIKDLKLRRKKNGIVVGVKLTKGMDKNDPRNPWYASWVEFG
                     TKKMPPHPFVRPAVQATGFQVLRIAESLIRMRIKMYDRGIKPSGI"
BASE COUNT         2026 a         1750 c         1745 g         1496 t
ORIGIN      
        1 atagtcccac atatagcata caacctattt tccatgatta ataattttgg ctaacggttc
       61 aggtattagc gatgtggcgg tttttcagca ccacagccga tacgaagaac cacagttgat
      121 ttaagaacaa atgtttaacc gaagcacgta agccgccata ttgctaatac cttgttagcg
      181 gctgcccttc tttcacaaat caaaataaaa atgagaagtt acaaaaacga attagaagca
      241 atagcaaacg atttgctaac acaaaacgca gaagcaaaag gtaacgagaa taagcctaat
      301 tactctaacc gtgattttat gaatgctaca ataatttttc agaccgcatt gatggacaaa
      361 atgtatgaca atcaagatta cgatggtatg tccgttgatg atagaatgaa gatggctgaa
      421 agttgtggtc ttgctttaag aaaattgata cacacctata ctggattaga tactcataag
      481 ttagaggagt ttctgtaagg ttgccgctaa ctattatata cacgccatat aattgtacaa
      541 acaaatacat atgcgcatcc ttataatcga aaagcccgaa atgacgcctg tatcctggtg
      601 gcgttgtttc atgccgttgc aaatgatgcg acggctcgat ccaaatttcg agtttgaaat
      661 aacagagaaa ttcagcgacg gcgacctaac ccgcttcgat gcgctactcc tgtttcgccc
      721 atcttatcca gtggaaatgt cggccattgc cacggcaaag aagatgggcc ttaaagtgat
      781 tgtcgattac gacgacgact ttcaaaacgt gccagaattt tcgccggtgt tcggtaaaat
      841 aatggagccg gagcgccaaa acgtagtacg ggcggttgcc gccacggcgg atatggtttg
      901 gacaactact gaggctattt caacggctat gcagcgtcct gatgcgcacg ttattccgaa
      961 cgccgtgcct gaaatatgga taccgaagaa acccgccaaa aacaccaaat cggcatcatg
     1021 gcgcgggcgc gaaatgcaat atatggatgt ggtatttcag ggcattgttt cgggatggta
     1081 caaccaaatc gcaactttgc cggaggtgtg gacctggatg ggatggatgc ctttcccgcg
     1141 tgcagaaggg ccaaagcacg ttttcaggga atacggaaac ataacgcagt acatggatta
     1201 cgtcagaaat gccggtatca atgtgttatg gaagccgctg gtagagtgcc aattcaacga
     1261 tgcaaaatcc aatataagtt ggatcgaagc gaccatagga ggcggcgtgt gcgtaacgaa
     1321 ttacgccggg aaaaaatggt gggagcacgc gctaccggat ttcacgttcg atgacaaggt
     1381 actgcgtgaa acctgggaag tcagcgcgca gtcagtatat gagcgccaca acctgaaagc
     1441 aaccgcacgc caaagattaa ccctgctaaa gcaattaatg aaatgaacct actccacaac
     1501 aaagaatatt tcaagcgcta tggcgaacac ctggagcgca ccggcagtcc gaaaaaggca
     1561 tgggcggcta cggaactcga attggagaat gaaaccggcg gattccggcg cttcatttcg
     1621 tataagtcct tcacagttaa attgtgcgca tacaggcatg gagagattaa caaaaacata
     1681 tcgcttaggt acgtcggaga agttgcaaca aaataaaccc aaccgcaact aatagaaacc
     1741 gaaaaaacgg cctgtttttg gcgcaatttt gcacaaaaca aggccataag tgcggatatt
     1801 cggctatgaa ataacatttt cgcgcaaaga aaagcgtaca tcagacttta ccggaggtaa
     1861 ctatctgatt tcaaacgctt cattttcagc gccatacggc acgaaggcca cagatcaaac
     1921 catccttggt ttgtcacctg tttgggccgc tatccggtat atttcagaag gtgttgctat
     1981 gcttcctttc gacgtgtacc gatctacgcc ggacggaaac gaacgtaacc caaaccaccc
     2041 gctccaatac ctgattagtg agcgtcctca cccgcactat tcaaggatag atttcctttc
     2101 cgcacttgtc gccaacgctt gtttgggcga cgggttcgcc cgtattcatt tcgacccgaa
     2161 ccaacggcct tatgccctgg aattattgcc gcgtgcaatc gtttcgatgc gttacggatc
     2221 tcagggacaa ctactgtacg acattatggg tagcccttcg ccgtccgatg tgtacagccc
     2281 gccggttatt gtggccacgc tgcaagatta cgaaattata catattaagg gcgtgacgct
     2341 atccggggtt aaaggtgaac gccttacgct cacccacaaa gacggtttcg gcgcggcgct
     2401 cagcgcacaa aagtacgtcg gcaatttctt cgacaatggc gcggcacctt ccggcgcact
     2461 cgtgtatcct tcgctactta cgaaagagca gattgacaag gccgaaaaga aagtccaaaa
     2521 caaatattca ggagccgaca atgccgggcg tgtaatgatc ctggacggag gcatgaagtt
     2581 cgaacggttt agcatggggc cgcaagaggc ggcgctcgtt gatttccgaa atttgtcggt
     2641 ggaagattgc agccggattt tcaaagtccc gcttcatatg ctttcgagcc ttgaccgttc
     2701 cacattctcc aatatcgagc agcaggaggg cgactattac acgcactgcc ttccggtgtg
     2761 gtcgcaaaag attgagcagg aattcaacta caaacttttc accgctttgg agcgctccaa
     2821 acgccgggct ttcgtcgctt tcgattacac cttcgcccgc atgggcgaca gtgatagcac
     2881 ggctaaaatg attgccgcca cggtgcaaaa cggcgtaatg acagccaacg aatggcgcaa
     2941 gcggttaaac cttcaaacca taccagacgg gaaccggacg tacatccagc aaaacatgat
     3001 gcctacagac atggtagacg aagttttgga aaataaatat gaaaaagata aaggcgaagc
     3061 ggcgcaagac caagcgccgg atgtcgaaga aaccgacaca ccagacgccg gaaccgataa
     3121 ccagaatatc accaatggca acgatactga gcaacccaac gattgaaagg cgctacttcg
     3181 attccaattt tgaagttcgc aaaatggatg acggcaagca tcgcgtatgc gggtatgccc
     3241 ttcgctttgg cgtttcctac gacatgggat ggttcactga ggaaatcagc cgcacggcgc
     3301 tggacaatgc cgatatgaca gatgtccgca tcctattcaa ccacgaccca aaccaaatcc
     3361 ttggccgcac gtcggcagga acggccacgg tgcgcgttga tgataacgga ttgtggtacg
     3421 aggctgagtt accgaatagc ccaaatggcg aaaatgtgcg ggtggccctg gagcgcggcg
     3481 atgtaactca aagttcctgg ggattcatgc tccggtacga tgacaataca cagggcgatt
     3541 tgtggcagcg caaaaacgga aaggatcacc gcaccattac cgacgtgtcg gcagtgtttg
     3601 acgcttcgcc ggtgacattc ccggcaaatc cggaaacatc ggcggcaaaa cgctcatatg
     3661 accaggtatt gaaacacgaa caaaagttaa aatcaaaaac ggctgagatc gactgcattt
     3721 tggccttgca ccaataacac aacaaaaatt tcgctatgaa aaatctgctt gaagcaaaac
     3781 aagcgcgaac ggatgccacg caacgcatta ccgaacttcg cgccaaagca acggcgggca
     3841 cctggggcga ggccgaacag tccgccttag acgccgccaa acgcgatttg gacgccgcag
     3901 acgctgccgt aaaacgtttt gaggatttcg aagccctgga aacccgcgct atcgcctgga
     3961 cacccaccgc aaccgcatcg aacccggtaa ccgtaaacgt gatttcttcg gaaaatcgcg
     4021 gcgacaaacc cgaaaacgtt gccaaacaat tccgccttct ggacgctgtc aaagaatcgg
     4081 cctacgggcg cggccttacc ggcgtaaatg cagagatcga ccaggagggc aaaaaagaag
     4141 cacgcgccgc aggtatcgac aagttcggaa cgggcagtat tacgcttccg tcgtttatgg
     4201 tacacggtga gaaacgcgat atgctcgcag gtacgaccac ggcgggcgga tacactgtcc
     4261 aaactgaagt tggtcaactg atagatttcc tctatccacg ccttacggtg aaggagatgg
     4321 gcgcaaccta cctcaccggc ctaaccggaa acatcgactt cccgcgcaac gatgccgccg
     4381 ccgcagtagc gcgcaccaca gagggagcga cctccaccga aacaagcccg acctttgacc
     4441 gcgtacaact tacgccggta cgctacgcag cctataccga cgtatccaag caagtagcga
     4501 tacaaagcac catcggaatg gaaaactttg tgcggaacgc gctcaacagc gcccttttcc
     4561 gagcgcttga aaccgaaata ttcaccaact ccggcagcaa cggccttttt gatatttctg
     4621 gcaccaatga tatcacgctt ggaaccaacg gcggcgatct cacatgggcc gacgtggtga
     4681 aattcgaaac ggaagtatcc gccgacaatg ccgatatggg ccgcatggga tacttgttta
     4741 ctccgcaggt ggccgggaaa ctgaaagcag ccaaacgcga tgtggccggt aacgggttca
     4801 tttgggaagg cccgaatatg gaagcccagg tgaacggtta tcgcgcaaaa gcatccaacc
     4861 tcctgccgaa aacgctttcg aaaggcggct ttacgtcggt tctacacggt ggccttttcg
     4921 ggaattggga ggaacttata atcggtcagt ggggcggcgt tgacctcctt atcaacccgt
     4981 acactaaagg caaagaagcc ttgattgaag tgatccttaa cgcatggttc ggctttgccg
     5041 ttcgccatgc tgcatccttc accaagtgcg acgaactcta tccgagctaa tggtacctgt
     5101 tttgtggcat agttccggtg tgcgcttcgg gtatgcgtac aacttcggag aaacgggcac
     5161 ggtgaaagaa tccgatctcc cgcgattgct ggcagcaaaa gcggtagcgg taattgagca
     5221 agcggcccgc gtacctgata agtaccaagc gcctgaaagc gcacaccacc aagccagaaa
     5281 atacgaacgc cgaaagaaat gacatataca gtaacgtccg gcccatcgag cgaaccgcta
     5341 acgacatccg aagtcaaaac atggctacgg gtgacaagta gcaccgacga caccttaatc
     5401 gaatcgttga ttaaggcggc acgcatgtac gcggaaaact tcacgggccg ggcgttattt
     5461 acgcagacca ttaaggaata cttcgacggg tggccgtatt gcggctgtgt tgaattagga
     5521 tttgggccgg ttcaatccat taccagcgta aaatactacg atactgacgg cacactccag
     5581 acggtttcat cttcgaatta cattacagac cttatttcac tgcctgggcg catagtaaat
     5641 cacccggact acgcatggcc ggaacttcaa acgcgcacta atcccgtaga aataatttat
     5701 gtggccggat acacggctac ggcctctatt cctgatggga ttaaagcggc catgaacttg
     5761 ctgatcggat tttggtacga aaaccgcgag gacatcccgg taaacaatca caatccatca
     5821 gttcggagcg ctgacatttt gttgcaccaa tacaagattc acctataaca tataatgcca
     5881 acaagcggaa acatatcggc gcgggtgcgc atcggcgaaa tggatgacga aatagttatc
     5941 caacgcgcta cgcttgcagc caacgattac ggagatccgg tacctacctg gtcgacgctc
     6001 gtttccacat gggcgaaggt ggaatggttg gaaaatagta gcgatgaggt ataccgggca
     6061 ggaattgaaa acgagtataa ccgcgtagac tttacgatcc ggtataactc cgatgttcgg
     6121 agcaaagacc ggatttcgtt cggcggaaat tacttcgaca tccaaaccgt acactacatc
     6181 gggcggcaac aattcaccaa gttacaaacg gtattgcggc aatgaaaggg aacggactaa
     6241 tactaacgca gtttgcgctc gatgccgatt tgctggttaa gaagttggag cgtatcagcg
     6301 ccgattttcg gaaggattcg cgtgaaataa tgtctaaggc gtctgagccg ctaatcgtgg
     6361 atattaagcg ccgggcaccc gtgaaaacag gacggcttaa aattgcaatc aaagacctga
     6421 aattaaggcg caagaaaaac gggattgttg tcggcgttaa acttacgaag ggtatggata
     6481 agaacgatcc tcgaaatccg tggtatgctt catgggttga gttcgggaca aaaaaaatgc
     6541 ctccgcatcc tttcgtccgc cctgccgtac aagcaaccgg atttcaggtg ttgcgcatcg
     6601 ctgaatcgct tatcagaatg agaatcaaaa tgtacgatag aggtattaaa ccatccggga
     6661 tatgagaata aaattcacaa cgacacacca caacggcgct acgctattca aaccaggcgc
     6721 ggttgtagag gtcgcaccgt caagcggcaa agccttaatc gaacaaggca aagccattga
     6781 ggtagacccg aatagtatcc cggtgtacga tggtaatttc cttacaaacg gatgcgcaga
     6841 ttattccgcg ctaaaattga acacagaaac aaaaactaaa aaataaacgc tatggcaacg
     6901 actggcagtg tagtttccta catcgtaaaa atattcaccg gctcaagccc aggcacagct
     6961 atctcttgcc tggtagatgc aaccctttca atgaccgcag aaacccgcga cgtttcg
//
LOCUS       DAOQGF010000151         6895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5358_length_6895_cov_6.568860, whole genome shotgun sequence.
ACCESSION   DAOQGF010000151 DAOQGF010000000
VERSION     DAOQGF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5358_length_6895_cov_6.568860"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PK937_14395"
     CDS             <1..1569
                     /locus_tag="PK937_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNH33812.1"
                     /translation="TCNDKSKQLSSGGPITKDNTSVEYYNTIFSLAESYKEKGVLWAG
                     SDDGLIHISRDHGATWNNVTPKDFPEGLISIIEPSYMDGGVCYVAATRYKFDDLQPYL
                     FKTTDYGKTWKRITQGIPNGAFTRVIRADPFRPGLLYAGTETGMFVSFNDGESWQSLQ
                     LNLPVVPIHDIAVQTREKDLVVATHGRSFWILDDLTPLHQIADAWAKQAGLLYKPRDA
                     YRMDGFQYSEPGVPVGTNPPNGVLINYYLKEPPKEEVRLEFLNAEGNLITAYSSKKDK
                     KGKTIEDSKEFYEKKKEKRMDVVPADSGMNRFVWDMRYPDAAEVAGAIMWGGNIRGPK
                     VVPGTYQVRMHIGSQSWTEKFEILKDPRVDTSPEDLKKQFELFSGIHKKLNEVHEAVN
                     RIRDVQKQITDYLNRIKDTTSATAMKKSAKPIQDSLSAIENELIQTKIKSSQDVLNYP
                     MRLNNKLAALAADVASADVRPTDAQYAVYEDLRAKADIRLAQLKPILENEIPRFNSEA
                     SLQTLPAINIDAKK"
     gene            1845..4745
                     /locus_tag="PK937_14400"
     CDS             1845..4745
                     /locus_tag="PK937_14400"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNH33813.1"
                     /translation="MAFIFAITLLGISSYAQSRFGLPEFVYYPPKAYEGFQQVWRAVQ
                     GHDGVMLFANGGPGYVLQFDGQRWRKAQLDIPGGAFSLDVNEAGTIWVGGHSDWGFLK
                     VDTSGMYRFYSLSSGLRDSIAGLTQIWNTITLDDEVYFGSFAGVIRYHNGESKLMKRK
                     NGFRLIFKVGNTIWAPIPGEGIYVLDRQSFEFKILKNSESLGERRIASIVPWPNGQYL
                     LLASRSEPLVYDPAAETLHTLKSSFDIRTFSPSSCIRLKDGNYLLTSSSHGIALMDSS
                     GSLLVQASMTDGLPTNNIKSAYQAKDGSIWLCTENGIISFDWHNPIRTRRDGQGFQGS
                     VTAMMRYNGRMMMGTTQGLYYEKTDSLSPLLSDWIRIPNIDFRITMLDTAWGYLAVQG
                     GEFVLLDTNFKTVFNNDFITQTVQRVPTHPDWLLSSSRNQLIRWNKKTKAREKLYDFH
                     DEIISLVLSHEKQDDTLRYWGGFLGKGAVEIMISRDGRYLYHRYIDMPYKGYTFVNYS
                     LGHLAANTMRGVYRFEEKNGSINVLEDNTLGPMINESKRNTFFIVADSTGYWVKAGAL
                     YHIKQINEQLWSYDSTIYLNTEVDPTGMLVDGRYLWVYGTDGVSRYDRSIGYDFNQSF
                     DSRIRTIKTRGDSVLYAGLPVSHRDDQNLKIDFRYNNLTFEFAASYFLDTKHLQFSWK
                     LQNFDADWTAWSSENRAIYTNLNEGEYTFRVKAMNVYGVIGREANYTFRILPPWYRTW
                     WFRVILLGLIGVILWRIYEYRVNRLLAVERLRIKIASDLHDDIGANLSKIAMYTDLAK
                     QSKHVVESIPILDKVGDLSRDVISSMSDIVWSIDARHDRIQDLGVRMQNFANELLGRK
                     GVVVRCEVIGADNSTTLPLQVKQNVYLIFKEAVNNIYKYAGATEVHIRLELHKNLITL
                     EVRDNGRGFEDGITMGGNGMKNMKMRSEVLGGRCTIESSHGVIVKIQIPVRY"
     gene            4761..>6895
                     /locus_tag="PK937_14405"
     CDS             4761..>6895
                     /locus_tag="PK937_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33814.1"
                     /translation="MRLRLFIVMTFFLLARLFGQDRIGVPKFHFYSPKEYNGGTQIWS
                     ITQDRDGIMYFASGGHNGGILQFDGQTWRKITLKDQRNVYSLAVDDTGAIWVGSFGDW
                     GFLKADTSGLMQYHSLAEAFGDSSNKIRQVWRTAVYNGEVFFGSLEGVIRFAKGKLQW
                     IRPTQSFHYMLPTARALWIKDKGVGLMRWDTESQAFVNAHGGDFFKSGIVVAMVSFEP
                     HEYWVVTADDGIYTYNDSTGVVDKLKTSIPLERLKLANAVRMKNEDIFVTSVLQGIFV
                     ITRDGRIRMHVDGDDGRGLSDTKFAFESRDGSLWFGHNNGLSVSDFYNPITYIRNEKG
                     INGAVTGVMSIGNKIFAGTTQGVWESEQDGKTPNILKFHKIKGTDFPVYGIDTMYTRI
                     VIYGGDFAILDANGKKLFHTWEFGSRTVVKLPLSENKILAAHRHQLYLIDVKTFSIKQ
                     IHRFDDEVVAILLDSVAGNDAVNYWFSYRNRSVRYIRIGRDGAVSVVAAKNIGGLNNY
                     SNLMNLGGRIVLNNDEGLYRLSFDQNSKTINTEWDSVINRCRGVGDINHIIFRNNEYW
                     ISKGFRFYKLIKPAQESWRLDSTVVVPGDPIYIDAQDSLSWFGGTEAITRWDHRVGRN
                     YKQVFPTLLRRIILNDDSLFWNGSRADQSNTQNETIIPYAFNSLMLYFSASHYHDISA
                     LRFSWKMEGFTDEWTDWSEESRAIYTNLN"
BASE COUNT         1962 a         1325 c         1694 g         1914 t
ORIGIN      
        1 acgtgcaacg ataaatcaaa acagttatct tccggcggac cgatcaccaa agacaacacc
       61 agtgtcgaat attacaatac gattttttcg ttggcggaat cgtacaaaga aaaaggagtt
      121 ctttgggccg ggagtgatga cggtttgata catatcagtc gtgatcatgg tgcgacatgg
      181 aacaatgtga cacccaaaga ttttcccgaa ggccttatca gcataatcga accgtcgtat
      241 atggatggcg gcgtctgtta tgtggcggcg acgcggtaca agtttgacga tctgcaacca
      301 tatcttttta aaactacgga ttacggcaaa acgtggaaac gtattacgca gggaattccg
      361 aacggcgcat ttacgcgtgt gattcgcgcc gatccgttcc gtccgggttt attgtatgcc
      421 ggcacggaaa cgggcatgtt tgtatcattc aacgacggtg aatcctggca atcgttgcaa
      481 ctcaatcttc ccgtggttcc gattcacgat attgccgtac aaacgcggga aaaagatctt
      541 gtcgtggcga cacacgggcg ttcattttgg atcttagacg atctgacccc gctccatcag
      601 atcgcggacg cgtgggccaa acaagcggga ttgctttaca aaccgcgaga cgcgtaccgc
      661 atggacggat ttcaatacag cgaacccggc gttcctgtag ggactaatcc tcccaacgga
      721 gtattgatta attattattt aaaagaaccg ccgaaagaag aagtaagact cgagtttttg
      781 aatgccgagg gtaatcttat aaccgcttac tccagcaaaa aagataaaaa aggcaaaacg
      841 atcgaggatt ctaaagaatt ttacgagaag aaaaaagaaa agcgaatgga tgtagttcct
      901 gccgattcag ggatgaaccg ctttgtgtgg gatatgcgtt atccggatgc cgcggaagtg
      961 gccggagcga tcatgtgggg cggtaatatc agaggtccca aagtcgtacc cggaacatat
     1021 caagtgcgca tgcatatcgg ttcgcaatca tggacggaga aatttgaaat tctcaaagat
     1081 cctcgtgtgg acacatcgcc ggaagattta aaaaaacaat ttgaactttt cagtggaatt
     1141 cataaaaaac tcaatgaggt tcatgaagcc gttaatcgga ttcgagatgt acaaaaacaa
     1201 ataacggatt atctgaaccg tatcaaagat acgacgagtg cgacggctat gaaaaaatcg
     1261 gccaagccga ttcaggattc gctttcagcg atcgaaaacg aattgataca aaccaaaatc
     1321 aaaagtagcc aggatgtact taattatccg atgcgactta ataacaaact ggcggccttg
     1381 gcggcggatg tcgcatcggc ggatgtgcgt cctaccgatg cgcaatatgc ggtttacgaa
     1441 gaccttcgag ctaaagcgga tataaggctt gctcaattga agccgatttt agaaaatgaa
     1501 attccacgat ttaactcgga agcatcgttg caaacgcttc cggcgattaa tattgatgca
     1561 aagaaataaa tttaacctat gaatttggtt tgactgtata ccccgcattt tttgcggggt
     1621 atttttttgt gcgattttgt aaacacattc caagattacg tgttcaagaa gcgcatacgc
     1681 gaattgaggt gcgaaccttt gccggcaaat aaactgcatg ggtttgtttt tttcaaatca
     1741 cggtatatga cctgtactgc atgaatatgt aggggtaaaa aagagtgtgg ttatctattg
     1801 tgcaataacc tacattttta tgcatgaaaa caatcctttc taaaatggct tttatatttg
     1861 ccatcacgtt gttgggtata tctagttacg cgcaatcgcg ttttggtttg cctgagtttg
     1921 tatactatcc cccaaaggcg tatgaaggtt ttcaacaagt ttggcgtgcc gtacaagggc
     1981 atgacggcgt catgctgttt gccaacggcg gaccgggtta tgtgttgcag tttgacgggc
     2041 aacgatggcg caaagcgcag cttgatattc cgggcggcgc gttttcactt gatgttaatg
     2101 aagccggtac gatttgggtt ggcggccatt ccgattgggg gtttttgaaa gtggatacgt
     2161 ccggtatgta tcgattctac tcgctttcat ccggtttgcg ggattcaatc gctggtctta
     2221 ctcagatttg gaatacgatc acattggatg atgaagttta ttttggttcg tttgccggag
     2281 tgatacgata tcataacggt gagtcgaagt taatgaaaag aaaaaatgga ttccgtctga
     2341 tattcaaagt tggtaatacg atttgggcgc cgattcccgg agaaggtatt tacgtgctcg
     2401 atcggcaaag ctttgaattt aaaattttaa aaaattctga atcgctcggt gaaagacgca
     2461 ttgcgagtat cgtaccatgg ccaaacggcc agtacctttt actcgcatca aggtcagaac
     2521 ccttggtata tgatccggcg gcagagacct tgcatacttt aaaatcttct tttgatatac
     2581 gcacatttag tccttcctca tgtattcgtc tgaaagatgg taattattta ttgaccagtt
     2641 catcccatgg aattgcgctg atggattcat ccggttcgct actagtccaa gcaagcatga
     2701 cagacggatt accgaccaat aatatcaaat cggcgtatca ggccaaagac gggagcatct
     2761 ggctgtgtac agaaaacggc ataatctctt ttgattggca taatccgata cgaacacgtc
     2821 gagacgggca agggtttcaa ggatccgtga ctgcgatgat gcgttataat gggcggatga
     2881 tgatgggtac gacccaagga ttgtattacg aaaaaacgga ttcactttcg ccgttgttat
     2941 ccgattggat acgcatacca aatatagatt ttcgtataac gatgcttgac acagcgtggg
     3001 gatatctggc agtacaggga ggcgagtttg ttttactcga taccaatttt aaaacggtat
     3061 tcaataatga tttcataact caaaccgtcc aacgcgtgcc tacccatccg gactggttac
     3121 tttcaagctc tcgcaatcag ttaatccggt ggaacaaaaa aacgaaagcg cgtgaaaagc
     3181 tgtatgattt ccatgatgaa ataatttctt tggttttatc tcacgaaaaa caagatgaca
     3241 cattgcggta ctggggcgga tttctcggca aaggcgccgt agagatcatg atctcacgcg
     3301 atgggcgtta tctctatcat cggtatattg atatgcctta caagggttac acttttgtta
     3361 attatagtct tggacatttg gcggctaata ccatgcgtgg ggtataccgg tttgaagaaa
     3421 aaaacggttc aatcaacgta ttggaagata acacgctggg tccgatgatc aatgaaagta
     3481 aacgaaatac tttttttatt gtcgccgaca gcaccggata ttgggtcaaa gccggagcct
     3541 tgtatcatat caagcagata aatgaacaat tatggtcata tgattcgaca atatatctca
     3601 ataccgaagt tgatccaacg gggatgttgg ttgacgggcg gtatttgtgg gtttacggaa
     3661 cagacggtgt gtcgcgttat gatcggagca tcgggtatga ttttaatcaa agttttgact
     3721 caaggatacg cacgataaaa acacgcggtg attcagtatt gtatgccgga ttgcctgtgt
     3781 cacatcgcga tgatcaaaat ttgaaaattg atttccgtta taacaatctg acgttcgagt
     3841 tcgcagcttc gtattttctc gacactaaac atctgcagtt ttcgtggaaa cttcagaatt
     3901 ttgatgccga ttggacagca tggagcagtg agaaccgcgc gatatatacg aacttgaacg
     3961 aaggggagta tacatttaga gtaaaagcga tgaatgtata tggggtcatc ggaagggaag
     4021 caaattatac atttcgcatt ttaccgccgt ggtatcggac atggtggttt cgtgtcatac
     4081 tgctgggttt gatcggtgtg attttatggc gaatctatga atatcgcgtt aaccgtctac
     4141 tggcggtaga aaggttgcga ataaagattg ccagtgattt acacgatgat attggggcca
     4201 atctttcgaa gattgccatg tacaccgatc ttgccaaaca aagtaaacat gtcgtggagt
     4261 ctattccgat actggataag gtcggcgacc tcagtcgtga cgtgatatcg agtatgagtg
     4321 atatcgtctg gtctatcgat gcgcgccatg accggattca ggacttaggt gtacgtatgc
     4381 aaaattttgc caacgaatta cttggacgta aaggggtagt ggttcgttgt gaagttatag
     4441 gggctgataa tagtaccaca ctgcctttgc aggtaaaaca aaatgtgtat ctgatattca
     4501 aagaagctgt aaataatatt tacaaatatg cgggcgctac tgaagttcat attcgtctgg
     4561 agttgcacaa aaaccttata actttggaag tgcgggataa tggccgcggt tttgaggacg
     4621 gcattacgat gggtggaaac gggatgaaga atatgaaaat gcgtagtgaa gttttgggcg
     4681 gacgatgtac tatcgaatcg tctcacggcg tgatagtaaa aatacaaata cccgtcagat
     4741 actgacaacg gaattgaatc atgagattac gactgtttat agtaatgaca tttttcttgt
     4801 tggcacgtct cttcggacaa gatcgtatcg gcgtacccaa atttcatttt tattcgccga
     4861 aagaatataa tggcggtacg cagatatggt ctatcactca agatcgcgat ggtatcatgt
     4921 attttgccag tggcggtcat aacggaggca ttttacaatt tgacggtcag acgtggcgaa
     4981 aaatcacact caaagatcaa agaaatgttt attcgcttgc tgtcgatgat acaggtgcta
     5041 tatgggtagg tagttttggg gattggggtt ttttgaaagc ggacacttcg ggtttgatgc
     5101 aatatcactc attggcggaa gcctttggtg attcgtctaa caagattcgc caagtctggc
     5161 gaacggcagt atataacggt gaagtttttt ttggttctct cgaaggtgta atacgatttg
     5221 ctaaaggcaa acttcaatgg atacgcccga cccagtcgtt tcattatatg cttcccacgg
     5281 cacgtgcatt atggatcaag gacaaaggtg tcggcctgat gcgttgggat acggaaagtc
     5341 aggcttttgt caacgcgcac ggcggcgatt tttttaaaag cgggatcgtg gtggctatgg
     5401 tgagttttga gccgcatgag tattgggtgg ttacggcgga tgacggtata tacacataca
     5461 acgattcgac aggagtcgta gataagctaa aaacatcaat cccgcttgag cgactaaaat
     5521 tagctaatgc cgtgcggatg aaaaacgagg acatctttgt taccagtgtt cttcaaggta
     5581 tttttgtcat aactcgcgat ggccgcatcc ggatgcacgt ggacggtgat gatggtcgtg
     5641 gtctttcaga taccaagttc gctttcgaat cgagagatgg aagcctgtgg tttggtcaca
     5701 ataatggttt gtctgtatcc gatttttata accctataac gtacatacgc aatgaaaagg
     5761 gaatcaacgg agctgttacc ggcgttatga gcataggtaa taaaattttt gcaggtacta
     5821 cacaaggcgt gtgggaaagc gaacaagatg gaaaaacgcc gaatattctc aagtttcata
     5881 aaatcaaagg aaccgatttt ccggtttacg gcattgatac catgtacaca cggatcgtga
     5941 tttacggcgg tgactttgcg atactcgatg ccaacggaaa aaaacttttt catacttggg
     6001 aattcggttc gcgtactgtg gtaaaattac cgctatccga gaataaaatc cttgcggcac
     6061 accgtcatca gttgtatttg atcgatgtaa aaactttttc aataaaacag atacatcgct
     6121 ttgatgatga agtggtggca atattattgg acagcgttgc cggcaatgac gccgttaatt
     6181 attggttcag ctatcgtaat cgatccgtgc gctacattcg catcgggcgt gatggtgcgg
     6241 tatccgttgt tgctgcaaaa aatataggcg ggttaaataa ttattctaac ctgatgaatc
     6301 tgggcgggcg aatagttttg aataacgatg aagggttata tcgcttatca tttgatcaga
     6361 actcaaaaac aatcaatacg gaatgggact ccgttattaa cagatgccgt ggcgttggag
     6421 atatcaatca tattatcttt aggaacaacg agtattggat ctccaaagga ttccgttttt
     6481 ataaactaat taagcccgca caagaatcat ggcgattgga ttccactgtt gtcgtaccgg
     6541 gtgatcccat ttacattgat gcacaggatt cgctcagttg gtttggaggt actgaggcta
     6601 ttacacgctg ggatcacaga gttggtagaa attataaaca agtttttccc acactattgc
     6661 ggcgtatcat actcaatgat gattcattgt tctggaatgg ttcgcgtgcc gatcagagca
     6721 atacacaaaa cgaaacgatc atcccgtatg cgttcaatag tctcatgtta tacttttcgg
     6781 catcacatta tcatgatatt tcagctctgc gcttttcatg gaaaatggaa ggatttaccg
     6841 acgaatggac ggactggagt gaagaaagcc gtgcgatcta caccaacctg aatga
//
LOCUS       DAOQGF010000152         6726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5561_length_6726_cov_6.868086, whole genome shotgun sequence.
ACCESSION   DAOQGF010000152 DAOQGF010000000
VERSION     DAOQGF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_6726_cov_6.868086"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PK937_14410"
     CDS             <1..1092
                     /locus_tag="PK937_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH33815.1"
                     /translation="DMSQLGSGTFQVQKWPAIGGPGQNWRKIRQRKPLTIEQANAIRE
                     RIRDAELVGAELWEQGSVLQHEEEKTNPNVSLAGGTPEFQINNNQLVGVGRFISEEDV
                     KVGRNVIVIGTTIAKKLFPFKDPIDQIVKINGHKYTVIGIYEEMGASASGSGDNSACI
                     PISAFVDQFGLKDNQGMPRSVNITVKARAGANVENVMEEVRGLLRQVRGVKPLDEDDF
                     DMYSNDTMIRAFGEMTVIIKLVAIVMGGIALIVAGIGIMNIMLVSVTERTKEIGIRKA
                     IGAKRKDIMQQFLIEAIVLCEIGGAIGIMVGMAIGNIVSILMSVPVKLPLDWAIIGLV
                     FCSGIGIAFGIWPASKASKLDPIESLRFE"
     gene            1213..1995
                     /locus_tag="PK937_14415"
     CDS             1213..1995
                     /locus_tag="PK937_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33816.1"
                     /translation="MKLIKFFLLMLSVWILIPGCASRVTVQSQRVTQNILINGHDDDW
                     EGQMQIIEKTGVAIGIRNDDDYLYLALSPLNRGMAMQWMTAGLTVWLDSTGGKNKRFG
                     IHFPIGLSDLGIPPMIMMGMPGERDTKRDNPLEKMSDRLLEEVEIIGPGKYDKQRIGT
                     KESTTLQVRLTHNPNGMFYELRIPLQNDPRNVCAINTRPGKNLGIGLETGKMERRRGM
                     PPMGEGGMGGPRGGGRPGGGRPPEMERAEPLSVWISTTLKGF"
     gene            2086..2601
                     /locus_tag="PK937_14420"
     CDS             2086..2601
                     /locus_tag="PK937_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33817.1"
                     /translation="MFDETFQKGENTVLREAGVHFRKAYELQMAGRLQDAIDEYQCSL
                     ALHPTAEALTFMGWAYSHLGDYAVAISKCEAAITVDPDFGNPYNDIGAYLISLQRLEE
                     AIPWLEKAKHARRYEARHYPFFNLGRIFEMQGRWFEAIAEYRGALHLYPNYQSAKDAV
                     TRLQILIDRRN"
     gene            2623..4425
                     /locus_tag="PK937_14425"
     CDS             2623..4425
                     /locus_tag="PK937_14425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNH33818.1"
                     /translation="MTRFVILLSLWVNGYAYAQTKALDVVYPREGVLIGSNDSMFVFG
                     CVKVAHQSLRINNMSTPTHEDGAFIQFLPITNVQKDSVFQMRFEIKTKDCTIVRYHRV
                     RIPLLPRPMDSDIPQIDSLSLKPSRVYRLQSGDDIPIACRATPHASVEFTIIDTTGKI
                     IDGPYPMAEYETDRMDDFPNAVYGEDRDTRPEPISGMYSATYRLCEGISYQRSRVAFR
                     VEKKKKSVTSVARGFIDLWPKHERLVAELNGPYHQPRTMPGQSFYYFLQGGLRILIDG
                     SIGSWRRLRFAENHSAWVSEDELTYAAPGTPVPASTIPVIRVRRDERTSVIKLTMSEK
                     LPYRVEQISPVMFKLFVYGGKADTDWIRFETEDPDIAQIHWSQPERDVFCVTVSLHSP
                     LSWGYEVRYESSNLVWRIYHKPKQTSLKGLRICIDPGHSKDPGSVGPRRTTEKQANVE
                     IAQWLKELLIADGAEVLMTHEDTTKGLTLYERVQFADANNCDLFVSIHHNALPDGVNP
                     LRSNFGHSVIYYHPHSRLLGEKILESLVNKVKLPNYGVFQGNIAVVRNSRMPAVLVEN
                     AFIMLPDQEKKIRDPKFQQTCAEAIRDGLRQFMK"
     gene            4440..5531
                     /locus_tag="PK937_14430"
     CDS             4440..5531
                     /locus_tag="PK937_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33819.1"
                     /translation="MPEQTGKTIIAVDDSELNLMMMQSFLEPMGHNVIVATNGKDALE
                     LIRTENPDLILLDVMMPEMDGFEVCRRIKSNDRTRHIPVIMITALDKVEDNVKGIEAG
                     AEDFLTKPFDAVILAARMKALLQSKALFDEIAKLERLKEDMTSMIVHDLRTPMSSIKM
                     SLEFLRDNIREEKLLDFISIASADIEDSLLLINNLLDIGKLEANKMEIRKEDTSLLDM
                     LRDIAGRAKPLLMRGGLQLSVQSDRDEIFCEVDKILVGRVVTNLLANAIKFAESKSMI
                     LLDATQLDNGDVVFGVVNQGTNIPESYKKTIFEKFGQAKDQHNKAGTGLGLTFCKHVA
                     EAHRGKIWVESPPKNFPSGAAFYVQLPLR"
     gene            5568..5978
                     /gene="rnpA"
                     /locus_tag="PK937_14435"
     CDS             5568..5978
                     /gene="rnpA"
                     /locus_tag="PK937_14435"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNH33820.1"
                     /translation="MISSTSFPPVTVMNDPIDEAFVNRRLPRRMIIRHKDAFERMFQN
                     GRRRNGRFVQLIIVPQDSAKASDLMVAFVCGKRIGHAVQRNLYKRRLREIFRKNKFHF
                     RGWHILLIAFPAIRHAKFEELEADVLRAAEGFRL"
     gene            5975..6691
                     /gene="rsmI"
                     /locus_tag="PK937_14440"
     CDS             5975..6691
                     /gene="rsmI"
                     /locus_tag="PK937_14440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNH33821.1"
                     /translation="MSGILYIVATPIGNLEDITLRAIRVLKEADTIACEDTRQSKILF
                     DHYGITARMVSLFEHNELRRIPEILQLLMSGKSVAVVSDAGTPTISDPAFKLVRAVRE
                     AGLPVSAVPGPSAALAAISVSGLPTDRFVFEGFLPHKKGRQTRWKTFAEEDRTIVLYE
                     SPFRVMKTLSEAREYLGERPMVVAREITKKFEETVSGTPSELIAHFEKHAPRGEFVIL
                     IAGKKYAEKALMSEEENEEN"
BASE COUNT         1812 a         1465 c         1738 g         1711 t
ORIGIN      
        1 gatatgagcc aattgggctc gggcactttt caggtccaga aatggccggc gatcggcgga
       61 cccgggcaaa actggcgaaa aatcaggcaa cgcaagccgc ttaccatcga acaggccaat
      121 gctatacgcg agcgtattcg tgatgcagag ctcgtcgggg ctgagctttg ggaacaaggt
      181 tccgtgttgc agcacgaaga ggaaaaaact aatcctaatg tatctttagc cggtggtaca
      241 ccggaatttc agatcaataa taaccagttg gtgggtgtcg gtcgttttat ttccgaagaa
      301 gatgtcaaag tcggtcgtaa cgtgattgtg atcggtacga cgatcgcaaa aaaacttttt
      361 cctttcaaag atccaataga tcagattgtc aaaatcaacg gtcataagta tacggtgatc
      421 ggtatatatg aagaaatggg cgcttcggct tccgggtccg gcgataacag cgcatgtata
      481 cctatttcag cttttgtgga tcagtttggt ctgaaagaca atcaaggtat gccgcgttcg
      541 gtgaatatta ccgtcaaagc gcgtgccggt gcgaatgtcg aaaacgtgat ggaagaagta
      601 cgtggattgc ttcggcaagt acgcggcgta aaacccctcg acgaagatga ttttgatatg
      661 tattccaatg atacgatgat ccgcgcattc ggcgaaatga cggtgatcat caaactggta
      721 gccatcgtaa tgggcggtat tgctctgatc gttgcgggta tcggtattat gaatatcatg
      781 ctcgtttcgg tgacggagcg taccaaagaa attggtattc gtaaagctat cggcgccaag
      841 cgtaaagaca tcatgcagca gtttttgatc gaagcgatcg tactgtgcga aatcggcgga
      901 gcgataggta tcatggtcgg tatggctatc ggaaatatcg taagcatttt gatgagcgta
      961 ccggtaaaac tgccgcttga ttgggccatt ataggactcg tattctgtag cggaatcggt
     1021 atcgcgttcg gcatttggcc ggcctccaaa gcatccaaac tggatccgat cgaatcgttg
     1081 cggtttgaat aattgaattt ttgaaaataa tcagagaaaa aggctgtgtt gatcacagcc
     1141 tttttttgtt ttaacttttt tggagctatc gcgtctaaaa agcaaaatta tcttataaaa
     1201 tagattgaga ccatgaaatt gatcaaattt tttcttttga tgttgtcggt atggattctc
     1261 atacccggct gcgcctcgcg cgttactgtt caaagtcaac gcgtaactca gaatatatta
     1321 atcaatggtc atgacgatga ttgggaaggt cagatgcaga tcatcgaaaa aaccggtgtt
     1381 gcgataggta ttcgcaacga cgatgactat ctttatttgg cattgtcacc gcttaaccgc
     1441 ggtatggcca tgcaatggat gaccgcagga cttacggtat ggttggattc gacgggagga
     1501 aaaaataagc gctttggaat tcattttccg ataggcttgt ccgatcttgg tattcctccg
     1561 atgatcatga tgggcatgcc cggtgagcgc gatacaaaga gggataatcc tttggaaaag
     1621 atgtccgata gattgctcga agaggtggag atcatcggtc ccggcaaata tgataaacaa
     1681 cgcatcggca ccaaagaatc aactacactg caagtgcgcc tgacgcacaa tccgaatggc
     1741 atgttttacg aattgcgaat accgttgcaa aatgatccgc gcaatgtctg tgcgattaat
     1801 acaaggccgg gaaagaacct cggcatcgga ttagaaacag gcaagatgga acgtaggcgc
     1861 ggtatgccgc cgatgggaga aggtgggatg ggcgggccgc gtggtggtgg ccgaccggga
     1921 ggcggtcgac ctccggaaat ggaacgagca gaaccgttgt ccgtgtggat ttcaacgact
     1981 ctaaaaggat tctaaataat ttaatatctt catttctatc cttgaatgtc gcaccgcgcc
     2041 gtggtagatt aaccggcaaa tcaaaaggtt caatttttaa acggcgtgtt cgacgaaaca
     2101 tttcaaaaag gcgaaaatac ggttctacgc gaagccggcg ttcattttcg caaggcatac
     2161 gaattgcaaa tggccgggcg cttacaggac gcgatagatg aatatcagtg ttcgcttgcg
     2221 ttgcatccga cggccgaagc gttgacattt atgggctggg cgtatagtca cctcggtgat
     2281 tatgcggtag cgatcagtaa atgcgaagcc gccatcactg tagatcccga ctttggtaat
     2341 ccttataacg atatcggcgc ctacctgatt tcgctccaac gattggaaga ggccatcccc
     2401 tggcttgaaa aagccaaaca tgcgcgtcgt tacgaagccc gacattatcc tttttttaac
     2461 ctcgggcgga tttttgaaat gcagggacgc tggtttgaag cgatcgccga ataccgtggc
     2521 gcgttgcatc tctatcccaa ttatcaatct gccaaagacg ccgtgacgcg cctgcaaatt
     2581 ctcattgacc gaagaaatta atccaaaaca acggatattc acgtgacacg atttgtgata
     2641 ctgctatctc tatgggtaaa cgggtacgcg tatgcgcaga ccaaagcgct tgacgtggtg
     2701 tatccccgcg aaggcgtact gatcggatca aacgattcca tgtttgtttt tgggtgcgtc
     2761 aaagtcgcac atcagtcgct tcggattaat aacatgtcaa cgccgacaca tgaagacggt
     2821 gcgtttattc aatttttgcc gataaccaat gtccaaaagg actcggtttt tcaaatgcgt
     2881 ttcgaaatca aaacaaaaga ttgtacgatc gtacgctatc atcgtgtgcg tattcctctt
     2941 ttaccacgac ccatggattc tgacattcca caaattgatt cgttatcact taagccgtcg
     3001 cgtgtgtacc gtcttcagtc gggtgatgat attccgatcg cctgccgtgc caccccgcat
     3061 gcgtcggtgg aatttactat tatagatacg accggaaaaa taatagacgg cccttatccg
     3121 atggctgaat acgaaacgga taggatggat gattttccca atgcggtata cggtgaagat
     3181 cgtgatacac gcccggaacc gatatcggga atgtattccg cgacgtaccg tctttgtgag
     3241 ggcatatcgt atcagcgttc acgtgttgcg tttcgtgtag agaaaaaaaa gaaatcggtc
     3301 acatccgtcg cgcggggttt cattgatctt tggcccaaac atgagcgcct tgttgctgaa
     3361 ctaaatgggc cttaccatca accacgtacc atgcccgggc agtcgtttta ttattttttg
     3421 caaggtggtt tgcgcatttt gatcgacggg agtatcgggt catggcggcg tctgcgtttt
     3481 gccgaaaatc actccgcttg ggtttcggaa gatgaattga cctacgcggc acccgggaca
     3541 ccggtgcccg ctagcaccat acccgtaatt cgtgtgcgtc gcgatgaacg tacatccgtg
     3601 atcaaactta cgatgtcgga aaaacttcca tatcgcgttg aacaaatttc gccggtaatg
     3661 ttcaaactgt tcgtgtacgg cggcaaagcg gataccgact ggatacgatt tgaaacggag
     3721 gatccggata tcgcgcagat tcattggtcg cagccggaac gcgacgtgtt ttgtgtgacg
     3781 gtcagcttac attctccttt gagctggggg tacgaagtgc gttacgaaag cagcaatttg
     3841 gtatggcgta tttaccacaa acctaaacaa acatcgctca aaggtttgcg catatgtatt
     3901 gatcccgggc atagcaaaga tccgggttct gtcggaccgc gccgtacgac ggaaaaacag
     3961 gctaatgtcg aaattgcgca atggttgaaa gaactgctca tcgccgacgg agcggaagta
     4021 ctgatgacgc acgaagatac gacaaagggt ttgacattgt acgagcgcgt gcagtttgcc
     4081 gatgcgaata attgcgattt attcgtcagc attcatcaca acgctttgcc ggacggcgtt
     4141 aatccgctcc gttcaaactt tggtcacagc gtgatttatt atcatcccca cagccggctc
     4201 ttgggagaaa aaatactcga atctctcgta aataaagtta aattaccaaa ctatggagtc
     4261 tttcagggca atatcgccgt agtgcgcaac agccgcatgc cggcggtgct cgtggaaaac
     4321 gcatttatca tgttaccgga tcaggagaaa aaaatccgcg atcccaagtt tcagcaaacg
     4381 tgcgcggaag ccattcgcga cggtttgcgg cagtttatga aataaacctt tggtgcttta
     4441 tgcctgaaca gacggggaaa actattatcg cggtggatga cagcgaactc aatctcatga
     4501 tgatgcagag ttttctggag ccgatgggac acaatgtcat cgtcgccacc aacggtaaag
     4561 atgcattgga attgattcgc actgaaaacc cggacctgat tttgttggat gtgatgatgc
     4621 cggaaatgga cggcttcgaa gtttgccgtc gtatcaaaag taacgacagg acgcgccaca
     4681 ttcccgtaat catgattacg gctttggata aagtcgaaga taacgtcaaa ggtatcgaag
     4741 ccggcgcgga agattttttg accaaaccgt ttgatgcggt gattctcgcc gcgcgtatga
     4801 aagcattatt gcaatcgaaa gcgttatttg acgaaatcgc caaattggaa cggctcaaag
     4861 aagacatgac aagcatgatc gtgcatgatc tgcgtacacc gatgagttcc atcaaaatgt
     4921 cacttgagtt tctgcgcgat aatattcgcg aagagaaatt actcgatttt atcagtatcg
     4981 cgtccgccga tatcgaagat tcgctgctgc tgatcaataa tttgctggat atcggcaaat
     5041 tggaagccaa caagatggag attcgcaaag aagacacttc tcttttagat atgctgcgtg
     5101 acatcgccgg acgggccaag ccgcttttaa tgcgcggagg attgcaactg agtgtacaat
     5161 ccgatcggga tgagatattt tgtgaagtgg acaaaatatt agtcggtagg gtggttacca
     5221 atttattggc taatgcgatc aaatttgccg aatccaagag tatgattttg ctggacgcga
     5281 cacagcttga caacggtgac gttgtttttg gcgtggtcaa tcaaggcacg aatattccgg
     5341 agtcgtacaa aaaaaccatt ttcgaaaaat ttggtcaggc taaagatcaa cataacaaag
     5401 ccggtaccgg cttaggtctg acattttgta aacatgttgc cgaagcgcat cgcggaaaaa
     5461 tatgggttga aagtccgccg aagaattttc cgagcggcgc cgcgttttat gtccagttgc
     5521 cattacgatg aatagattcc ttgtgacgaa aaaacaaggt ttgcgttgtg atttcctcta
     5581 catcatttcc gcctgtaacc gttatgaatg atccgataga cgaagctttt gttaaccgcc
     5641 gattgccgcg gcgtatgatc attcgccata aggatgcgtt tgagcgtatg tttcagaacg
     5701 gccgaaggag aaacggacga tttgtgcaac tgatcatcgt accgcaagac agcgcgaagg
     5761 catccgactt gatggtcgct tttgtgtgcg gcaaacgtat cggccatgcc gtgcaacgca
     5821 atctgtataa acgccgtttg cgtgaaattt tcaggaaaaa caaatttcat tttcgcggat
     5881 ggcatatttt attaattgct tttccggcga ttcgccatgc gaagtttgaa gagttggagg
     5941 cggatgtttt gcgtgccgcg gaaggattta gattatgagc ggaatattat acatcgtcgc
     6001 tacaccgatc ggcaatctgg aagatatcac gctgcgagcg atccgtgtgc tcaaagaagc
     6061 cgatacgatc gcctgcgaag atacgcgtca atcaaaaatt ttattcgatc attacggcat
     6121 cacggcacga atggtcagtc tttttgagca taatgagctc cggcgaatac cggagatatt
     6181 acaactgctg atgtccggta aatccgtggc cgtggtttcc gatgcgggta caccgacgat
     6241 ttcagatccg gcattcaagt tggtacgtgc cgtgcgggaa gcgggtttac cggtttccgc
     6301 cgtgcccgga cccagcgccg ctttggcggc tatatcggtg agcggtttac ccacggaccg
     6361 atttgtattc gaaggttttt taccgcataa aaaaggccgc caaacacgat ggaaaacgtt
     6421 cgcggaagaa gaccgcacga tcgttttgta cgaatcgcca tttcgtgtga tgaaaacact
     6481 gtcggaggcg cgcgaatatc tcggcgaacg ccctatggtc gtagcgcgcg agataaccaa
     6541 aaaattcgaa gaaacggttt caggtacgcc gtcggaattg atcgctcatt ttgaaaagca
     6601 tgcaccacgc ggcgaatttg ttatcctgat tgccgggaag aaatacgccg aaaaagcgtt
     6661 gatgtcggaa gaagaaaacg aagaaaatta agtttgtaaa taaatacaga aaggttacgt
     6721 cattgg
//
LOCUS       DAOQGF010000153         6721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5572_length_6721_cov_4.988149, whole genome shotgun sequence.
ACCESSION   DAOQGF010000153 DAOQGF010000000
VERSION     DAOQGF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5572_length_6721_cov_4.988149"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PK937_14445"
     CDS             complement(<1..1631)
                     /locus_tag="PK937_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33822.1"
                     /translation="MAVAAQGFHQTHCFTGSWDVHSGMKTTKLSGPVIADNYDQGIRL
                     AMRLIEARENLADGSGMDITDWAQSVFGTERLVRLIETEPFSEKIYAPVIREEIERLA
                     KTWYPDVGKRHIVSINKLEDEFDVHVADDEAPSSPLATVSADQVLDFLSSRLTSAQLE
                     YLRRILKNSFGEDRDFSWNERIFRLLAESESYLQSNIDRMMAMYPDGPPLQENFNTGQ
                     TFSDEQIITCYKNVFLGINARFPIHFLATDGNRRAAILTQFAVEHILKTRPLDALQEY
                     TARDFSAIGLRNVARFFNYSLNRIIRNAYPDLVMPWEMAHVEDGFWTEEKNRTMAIRW
                     LIEEKLGIAKTDIPKALRDDRVTKSSFTQNGLSYMFVQYYKSVSRCIGAAYPEFMPWE
                     LGSVPNAFWQGEEGRQNVVKAIHWLMRRMQIIPAEIPERIKDKTINRELFSRFGLATV
                     FERIFKKNMFQVFETAYPGRFEIWEIGKVATDYWEDLLHAYRASLWIAKKEGIPENGI
                     HAALRSGMLSRESFAKYGLGSMLKRTFDNNLRKAY"
     gene            2018..2581
                     /locus_tag="PK937_14450"
     CDS             2018..2581
                     /locus_tag="PK937_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33823.1"
                     /translation="MLKRVVLSVATLTLAAFVLIGCGGPPKELIEKATVAMKKATEAK
                     ADVYAKEAFDAANAALNKGAEAVKKSDWDGAKKAYTEAAAQFEAAAKAAPEAMKAKME
                     EAKAGIAAAKKAWEDMGKDKMLMAATKLMKKDDKAKYDAMGKEVEGAVKGAEGMMETD
                     PMGALSSLAMATEKMDAMKKMAMPAKK"
     gene            2673..3230
                     /locus_tag="PK937_14455"
     CDS             2673..3230
                     /locus_tag="PK937_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33824.1"
                     /translation="MLKRMIVSALTVAMTAFVLIGCGGPPEQEIAKAKAAAKKASEAK
                     AADYASESFAKAEAKMKEADGLVAKKEWDKSKAAYVEAEKLYNEAATAAPAGMEQVKT
                     STDDFIKNCETTWTKNEKAMMAAAGKLKAADKKEVETTYAGCKAKLEEAKKQFAAGDF
                     KASRATADEAAMMYGKINEKLTAKK"
     gene            complement(3294..4067)
                     /locus_tag="PK937_14460"
     CDS             complement(3294..4067)
                     /locus_tag="PK937_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNH33825.1"
                     /translation="MDYLLIAIAAFTASALTFFSGFGLGTILMPVFALFFPVHTAVAL
                     TAVVHFLNNIFKLGLTGFFADKAVILRFGIPAIVAAFAGAELLTWMSASEPVASYYLG
                     HTIFYIRWEKIMMAIVMIIFALLEIIPTWSQIHFDPKYLLYGGILSGFFGGLSGHQGA
                     LRSAFLLRLGLSKESFIATGVVIACMIDITRLGVYGLTVFNENIVAHWPPLLTAISCA
                     FGGAFLGNRFLKKMTIALLQKSVALFLIALACALATGII"
     gene            4204..4971
                     /locus_tag="PK937_14465"
     CDS             4204..4971
                     /locus_tag="PK937_14465"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNH33826.1"
                     /translation="MKIRIFCVYIALSLSCVVLAQVNTENLRKYNADRGFHHTVGFSL
                     GLQEGNSESLSMKSNYRLDWLTSAYYSFFAANYERAESRGKLYSHKGFMHLRVTRNLS
                     DYVIAEGFTQKEFNAFIDLKDRQLIGSGLRYEIMRTDSGRKSPILVWAVGAGLMWERE
                     MYEHNEPTARKLRSTNYLSIRWNPDDRLAFVATTYYQADTRAFSDYRFLHNTSMSIAL
                     WKKLSFVTAFNVRYDHRPAPGIRTYDVELSNGVNIVF"
     gene            4975..>6721
                     /locus_tag="PK937_14470"
     CDS             4975..>6721
                     /locus_tag="PK937_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH33827.1"
                     /translation="MASKTPKRVSSKKKSPQKTLRKSHSTRQRDALLHLYRLRKGFDA
                     LVRAAVRISADLQSIDRVSVWLFTPERSAIQCVALYSREKKKYFQGDVLLASKYKPYF
                     KALNSGRSLLANNAQKDPRTTAFTKDYLLPNGIGAMMDVPIWQDGRVIGVLCHEHVGG
                     SRRWTRREEEFANAVADCITGSKHAEERRQAEIRYHALLEASDDMVFIEDYYRNVLYI
                     NPAVEKHTGYTAADYDFNVHQHPLIYPEDLPNVNRFIADFIQSEAMFSKTLENRFVDR
                     WGRLRWHSSVLSKIEYLQKPAIQFVVSDITGQKFALDALHRSEERFTMLVRQLRDLIT
                     IVSADGTIRYESPSIFPMLGYTQDELLGRNAFDQIHPEDLEVLQKTFESLLRAPGESA
                     TAMYRFLHKNGTWRHCESVGVNLLLDASVEGILITTRDVTERVEAEQLLKEREERYRK
                     LVESFHDILFITDFKRNMLYINPAMERLTGYTLNDFRFPPDDNPFVHPEDFKRVNAFI
                     DEFVQSAKTHSSIIENRFFDRWGTMHWYSSVISKIEYDDQPCLQFICRDISDVRRSEE
                     ALTQSDQKYRAFIEQS"
BASE COUNT         1949 a         1466 c         1566 g         1740 t
ORIGIN      
        1 aagtaagcct tgcgcaggtt attatcaaaa gtacgcttga gcatgctgcc gagtccgtat
       61 ttggcaaagg attcgcgtga aagcatgccg ctgcgcaaag ccgcgtgaat accgttttcg
      121 gggatgccct cttttttagc aatccacagc gatgcgcgat aggcatgcag cagatcttcc
      181 caatagtccg tcgccacttt gccgatttcc catatttcaa aacgaccggg atacgcggtt
      241 tcaaaaacct gaaacatgtt ctttttgaaa atgcgttcga aaaccgtggc gaggccaaac
      301 cgactgaaga gttcacgatt aatggttttg tctttgatac gttcgggtat ttcggccggt
      361 atgatttgca tgcgccgcat gagccagtgt atcgctttca cgacattctg ccgtccttct
      421 tcaccttgcc agaaagcgtt cggtacgctg cctaattccc acggcataaa ctcagggtat
      481 gccgcaccga tacaacgtga aaccgatttg taatactgca caaacatgta cgacaatccg
      541 ttttgtgtaa aggatgattt ggttacgcga tcatcccgca gagctttagg aatatccgtt
      601 ttggcaatgc cgagtttttc ttcgatcagc caccgaatgg ccatcgttct gtttttttct
      661 tccgtccaaa atccgtcttc gacatgcgcc atttcccaag gcatgacgag atcaggatag
      721 gcattgcgaa taattcgatt cagcgaataa ttgaaaaatc gagccacatt gcgcaaaccg
      781 atcgcactaa aatcgcgcgc cgtatattct tgcagtgcgt ccagcggtcg ggttttcaga
      841 atatgttcga cggcaaattg tgtcaaaata gcggctcttc ggttaccgtc cgtagctaaa
      901 aaatgaatcg ggaaacgtgc gttgattccc aaaaaaacat ttttatagca agtgatgatt
      961 tgttcgtctg aaaaagtttg cccggtgtta aaattttcct gcaatggcgg tccgtcggga
     1021 tacatcgcca tcatacgatc tatgttagac tggagatagg attccgattc ggccaaaagc
     1081 cggaaaatac gttcattcca cgaaaaatcg cggtcttcgc cgaaagaatt tttaagaatg
     1141 cggcgcaggt attccagttg agccgaggtc aaacgcgagc tgagaaaatc cagtacctga
     1201 tccgccgata ccgtcgcaag cggtgatgaa ggcgcttcat catcggcgac atgtacgtca
     1261 aactcatctt cgagtttgtt gatagaaacg atatggcgtt tgcctacatc aggataccac
     1321 gtcttggcta atcgttcgat ttcttcgcga ataacagggg cgtatatttt ttccgaaaaa
     1381 ggttctgttt cgatcagtcg cacaaggcgc tctgttccaa aaacagactg cgcccaatcg
     1441 gtaatatcca tgcccgaacc atcggctaga ttttcacgtg cttcgatcag acgcatggcg
     1501 agccggatac cttgatcata gttgtcggcg atcacagggc cggaaagttt ggttgttttc
     1561 atgcctgaat gtacatccca cgaaccggta aaacaatgtg tttgatgaaa cccctgcgca
     1621 gctacggcca tagtctatct ccgattattt tttaaactcc cggttcgaaa aacgatgccg
     1681 ggagtttttt taaccaacag aactcaaact cttagataaa gcaggcttat atatgattag
     1741 gcatggagat atacgatttt cgctgtatcc tacataacta atatcgcagt aagcatgtaa
     1801 gaatgcgtaa aatggtaagt gattatcgga aatgatgctg aaaataaaga agtttagatc
     1861 aaaaaacagc ttgcttttgc cggcatgaga tcatatattt gcgcagattt tgtaaataag
     1921 tggttttttt aaccctaaac accataaaaa taaaaactta gcacttaggt agattgggtg
     1981 ttggtagcct taagtgcaag cattaaagga gaaacccatg ctgaagcgtg ttgtattgtc
     2041 cgtagcgacg ctgaccctcg ctgcattcgt tctgattggt tgcggcggtc ccccgaaaga
     2101 attgatcgaa aaagccacgg ttgctatgaa aaaagctacc gaagctaaag ctgatgtgta
     2161 tgctaaagaa gcgtttgatg cagcgaatgc cgctttgaac aaaggtgctg aagctgtgaa
     2221 gaaaagcgat tgggacggtg cgaagaaagc ttatacagaa gctgccgctc agtttgaagc
     2281 tgctgctaaa gccgctcctg aagcaatgaa agctaaaatg gaagaagcca aagccggtat
     2341 tgcggccgct aaaaaagctt gggaagacat gggcaaagac aaaatgctga tggctgccac
     2401 caaactgatg aaaaaagacg acaaagccaa atatgatgct atgggcaaag aagtcgaagg
     2461 tgctgtaaaa ggcgctgaag gcatgatgga aaccgacccg atgggcgctc tttccagcct
     2521 cgctatggct actgaaaaaa tggatgccat gaagaaaatg gcaatgcctg ctaagaagta
     2581 attcaaattt aattttgaat aaaccccgga tactcgtatt cggggttttt ttgtaaatat
     2641 tcccataacc cttaaattaa acggagaatg ctatgttaaa acgtatgatc gtatccgcgt
     2701 tgactgtcgc tatgacagca tttgtactga tcggttgcgg cggtcccccg gagcaggaaa
     2761 ttgctaaagc taaagctgcc gcgaaaaaag cttctgaagc taaagctgct gattatgcca
     2821 gcgaatcgtt tgcaaaagcc gaagctaaaa tgaaagaagc cgatggtctt gtggctaaga
     2881 aagaatggga taagtccaaa gccgcttacg tcgaagccga aaaactctat aatgaagccg
     2941 ctaccgcagc tccggccggt atggaacaag tgaaaacatc cacggatgat ttcatcaaaa
     3001 actgcgaaac gacctggacg aaaaacgaaa aagccatgat ggcggctgcc ggcaaactca
     3061 aagcggcgga caaaaaagaa gtggaaacaa cctatgccgg ttgtaaagct aaattagaag
     3121 aagccaaaaa acaatttgcc gccggtgatt tcaaagcttc acgcgctacg gccgatgaag
     3181 ccgctatgat gtacggcaaa atcaatgaaa aattgacagc taagaagtaa taactgaaaa
     3241 gctgaatttt tttaaacccc gggatttttt agttccgggg ttttttattg tgatcagatg
     3301 atacccgtgg ccagagcgca ggcaagggcg ataagaaata aagccacgct cttttgtagt
     3361 aaagcaatgg tcattttttt caaaaaccga tttcccaaaa acgcgccgcc aaatgcgcag
     3421 gagattgccg tgagtagtgg cggccagtgt gctacgatat tttcattaaa aacggtaagt
     3481 ccgtaaacgc ctaatcgcgt tatgtcaatc atacatgcga tgacgacacc tgtggcaata
     3541 aaactttctt tactcaggcc taaacgaaga agaaacgcgc tacgcagggc gccttgatgt
     3601 cctgataacc cgccaaaaaa accgctgaga attccgccgt atagtaaata tttaggatca
     3661 aaatgtatct gtgaccaagt cggtatgatc tccagcaatg caaaaatgat catgacaata
     3721 gccatcatta ttttttccca tcgtatatag aagatcgtat ggcctaagta ataactcgca
     3781 acaggctcag atgcggacat ccatgtaagc agttctgctc cggcgaaagc agctacgatg
     3841 gccgggatgc caaaccgcag gatcacggct ttatcggcaa agaatccggt aagtcccagt
     3901 ttaaaaatat tatttaaaaa atgaacaaca gcggtcaatg cgacagctgt gtgaaccgga
     3961 aaaaacagag caaagaccgg catcaaaatt gttcccagcc cgaaacctga gaaaaaagta
     4021 agcgccgatg ccgtaaaggc cgcaatagca atgagtaaat aatccatcat aatattatac
     4081 cttgagaata ataaagaggt tattatcctt cttcaacaga tttcctatga aaaatatttt
     4141 acataacacc gattttatta tatattgtcc ggcttaccct ccttgtctaa aggttctaca
     4201 tatatgaaaa tcaggatttt ttgcgtttat atcgcattga gcttgtcgtg tgtcgtattg
     4261 gcgcaggtaa atactgaaaa tttacgcaag tataatgcgg atcgaggatt ccatcatacc
     4321 gtaggtttca gcctcggcct ccaagaaggt aattccgaat cactcagcat gaaaagtaat
     4381 taccgcctgg actggcttac ctcggcatat tattcttttt ttgccgcgaa ctatgagcgt
     4441 gccgaaagcc gtggaaaact gtattcgcat aaagggttta tgcatttgcg tgttacccga
     4501 aatctgagcg attacgtgat tgccgaaggt tttacgcaga aagaatttaa tgcgtttatt
     4561 gaccttaaag atcgtcaatt gatcggcagc ggtctacgct atgaaattat gcgaacggat
     4621 tcggggcgca agtctcctat attggtatgg gctgtcggcg cagggttgat gtgggaacgt
     4681 gaaatgtatg agcataatga accgaccgcc cgaaaactgc gctccactaa ttatttatcc
     4741 atacgatgga atccggatga tcggttagca tttgttgcga cgacctatta tcaagcggat
     4801 acgcgcgcgt tttccgacta ccgctttttg cataatacca gtatgagcat cgccctatgg
     4861 aaaaaattga gttttgtgac ggccttcaat gtgcggtatg atcatcgccc ggcgcccggg
     4921 attcgtacgt acgatgttga attaagcaat ggtgttaaca tagttttttg agttatggcc
     4981 agtaaaaccc caaagcgagt ttcatctaaa aagaagagtc cccaaaagac tcttcgaaaa
     5041 tcacatagca cgcgacagcg cgatgctctt ttgcatctgt atcgtctgcg taaaggtttt
     5101 gacgctttgg tgcgcgcggc ggtacgtata tccgccgatt tgcaatctat tgatcgcgtc
     5161 agtgtttggt tattcacacc ggaacgatcg gctatccagt gtgtcgcact ttacagccgg
     5221 gagaaaaaaa agtatttcca aggggacgta cttttagcgt ctaaatataa accttacttt
     5281 aaagcattga attccggccg ttccctcctg gcaaacaatg ctcaaaaaga tccccgcact
     5341 actgctttta cgaaggatta tcttttgccc aatgggatcg gtgcgatgat ggatgtgccg
     5401 atctggcagg acggacgtgt tatcggtgtt ttgtgccatg agcatgtcgg aggatcacgc
     5461 cgatggacac gacgcgaaga agagtttgcc aacgctgtag ccgattgtat caccggctca
     5521 aaacatgcgg aagaaaggcg ccaagccgaa atacgatatc acgctctact cgaagcgtcg
     5581 gatgacatgg tatttatcga ggactactat cgcaatgtat tgtacatcaa ccccgctgtc
     5641 gaaaaacaca cgggatacac ggccgcagat tatgatttta atgtacatca acatccgttg
     5701 atttatccgg aggatttacc caacgtcaat cgtttcatag cggattttat acaaagcgaa
     5761 gccatgtttt ccaaaacttt ggaaaaccgt tttgttgatc gttgggggag attacgctgg
     5821 cattcgtccg tacttagtaa aatcgaatac cttcaaaaac cggccattca gtttgttgtc
     5881 agcgacatca ccgggcaaaa atttgcctta gacgcattgc atcgcagtga agaacgattt
     5941 acgatgctgg tgcgccaact gcgcgatctg atcacgatag tttcagcgga cggtacgatc
     6001 cggtacgaaa gcccttccat atttccgatg ctcggttata ctcaggacga gttgcttggg
     6061 cgcaatgcat tcgatcagat tcatccggaa gaccttgaag tattgcaaaa aacttttgaa
     6121 tccttgcttc gtgcgccggg cgaatcggct acggctatgt accgtttttt gcacaaaaac
     6181 ggaacgtggc gtcactgtga aagcgtcggt gtcaatctgc ttttggacgc atccgttgag
     6241 ggaattctca ttacgacacg cgatgttacc gaacgtgtgg aagctgaaca gttgctcaaa
     6301 gaacgggaag agcgttacag aaaactagtg gagtcgttcc atgatattct tttcatcacg
     6361 gatttcaaac gcaatatgct gtatatcaat ccggcgatgg aacgtcttac cggttataca
     6421 ctcaacgact ttcgttttcc gccagacgat aatccctttg ttcacccgga agattttaaa
     6481 agagttaatg cgtttattga cgagtttgtg caaagcgcga aaactcattc aagcattata
     6541 gagaatcgtt tttttgatcg ttggggtacg atgcattggt attcatcggt gatttccaaa
     6601 atagaatacg acgatcaacc gtgtctgcag tttatttgcc gtgatatttc cgatgtacgt
     6661 cgttctgaag aagcgttgac ccaaagtgat caaaaatacc gcgctttcat cgagcaaagt
     6721 a
//
LOCUS       DAOQGF010000154         6613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5714_length_6613_cov_6.536749, whole genome shotgun sequence.
ACCESSION   DAOQGF010000154 DAOQGF010000000
VERSION     DAOQGF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5714_length_6613_cov_6.536749"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PK937_14475"
     CDS             <1..410
                     /locus_tag="PK937_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33828.1"
                     /translation="KLVFLYISTLGTYTIYWFYKHWKLIEPRMEEKIMPFWRAVFFIF
                     FAHSLFGRVETEARKRSLALSWSAGSLAGIFVILTVAQRIVDRISMRSQTVGYVEALS
                     IGLMLSCIWPIIVVQKTANQAILRICWLLLCLF"
     gene            complement(340..1314)
                     /locus_tag="PK937_14480"
     CDS             complement(340..1314)
                     /locus_tag="PK937_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNH33829.1"
                     /translation="MDSLFSLKLKIEGGLAWLTFDYPGEKFNKLSTPVMQALNQALDY
                     LASQAPSIQGCIIHSAKDGIFIVGADIDEIKNITDPIIAEAAAAQGQSIFTKIEKLPF
                     PTVCLIHGACMGGGTELALACTYRIATDSPKTKIGLPEVMIGIVPGFGGTQRLPRLIG
                     LQRALPMILSGKAYDGKNALRNGVVDKVIPSEFAERYAAAFVQEIISGKTTGYVKRRK
                     PSGLINLLLESVGKPLIYSQARKSVLKETKGQYPAPLKALDAVREGYSQSLENGLKIE
                     ARYLGEMVASPISKNLIRIFRLTEQIKKDTGVTNKSVKSPDWRFSARL"
     gene            complement(1390..2388)
                     /locus_tag="PK937_14485"
     CDS             complement(1390..2388)
                     /locus_tag="PK937_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNH33830.1"
                     /translation="MMASVDIKALNERIYIESGFIDNILKEVGLVIVGQKYMIERLLV
                     ALLSNGHVLLEGVPGLAKTLAIKTLSETIHTKFQRIQFTPDLLPADLLGTLIYNQKEG
                     NFYTRKGPVFSNLILADEINRSPAKVQSALLEAMQERQVTISDTTYPLPEPFLVLATQ
                     NPVEQEGTYPLPEAQVDRFMLKLKVGYPTRDEELEIIRRNATTAPQLKVKPVATPEEI
                     LRARKTVNEIYMDEKIENYIVDLVFATRQPEKYGLPELKPLIHFGASPRASISLTLAS
                     KAYAFLKRRGYVIPEDVRAVAMDVLRHRVILTYEAEAEELTTEEIITQVLNKVEVP"
     gene            complement(2413..4383)
                     /locus_tag="PK937_14490"
     CDS             complement(2413..4383)
                     /locus_tag="PK937_14490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH33831.1"
                     /translation="MFNFSRTLLRDVILYAVLVAIFIAIFLIGEYISDSYFPRTSDYV
                     IFFFKALVFILIYEPIRKLTELYIRKIVFSYYYRRQEDLRDLDARLVSTMGYHEMADI
                     ITERISGLLNVKYAAIYLRIPEFFSLVSSYGRSDLITKRIKADMAVYDALLSGKRVFT
                     INELFVMHQTVLTDDALKVFQHEGCKYIIPMLKKDGISALLVLGEETQIRYDLSDEDK
                     KILWQSMQRVGNTLESTRLYSQLQKSLVEKEIVLEIAKKFNGEIHTEKLLDNILDAVK
                     SIVPYDAAGIFLVNEVTQEIESAVMRGYDPEVMEQIRMKVGTGLIGHVAIIGKPVIVK
                     DVNFDEHYVSVRDNTRSEITIPIKDSDRTIGVLNLESDMLGAYHEGFLDILNAIAGEA
                     GIAIQNAQLNEQAIRNEELQKELKIAGKIQQAILPQQLPRIPNLQITAKSLPCYAVGG
                     DFYDVLKLNDHQVTVSIGDVSGKGVPGAIMMSVLYTSYRGMVHEYKTSWEVVNSLNNL
                     LCTHTAEGRYATFFHCIIDFERLIMYYTNAGHNPPMIRKESGEWIKLREGGIVLGFIP
                     DQDYRQMTQPLDPGDIIVLYTDGITEVFNEAEELFGEERLKEVIENNRETSAKNIQDE
                     IIAAVSTFAPDQEMQQDDITLVIIKIEKSTLV"
     gene            4574..6457
                     /locus_tag="PK937_14495"
     CDS             4574..6457
                     /locus_tag="PK937_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH33832.1"
                     /translation="MSAHDAQSGKRHVTEEEAREVAESARESKWENPSFLREIFLGNL
                     RLDLVHPYPEPKGINRPEFQQFYTEFKKFIEEEVDSDAIDREGKIPMAVRDRLKAMGA
                     FGMKIKKEYGGLEFSQAEYNKIMELICTKDGNVIALLSAHQSIGVPQPLMLFGTDEQK
                     QKYLPRLAKGEISAFALTEPEAGSDPAKLHTTAVESEDGQSYILNGEKLWCTNGTIAD
                     IIVVMARHPEDGAITAFIVETKWPGVKVDHRCYFMGLKALENAVLSFKDVRVPKENII
                     WGRGKGLKLALITLNTGRLTLPAGAAGTAKACLHVAREWASKRVQWGQPIGKHEAIAQ
                     KIADMASMTFAMESVASLSTAMADKKLDIRLEAAVGKMYNTEAGWKIVDDTLQIRGGR
                     GYETADSLRARGEDPVAVERMMRDFRINLIFEGSSEIMRLFIAREAVDKHLEVAWKMI
                     DPKSSIGEKLGALPKIALFYGIWYPTRWLGWGWWPRFSEFGPLATHMRFVERKTRKLS
                     REIFHGMMRHQAKLAYKQAFLFRAVDIGAELFAMSATMSRAVKMAKEGNSDAMKLADL
                     FCRNARRRVEVSFKAMWNNDDNAKYNMAKDILNNKYGWMEAGIAESKESKTKSQSAPT
                     VAA"
BASE COUNT         1735 a         1461 c         1624 g         1793 t
ORIGIN      
        1 agaagctggt gtttctctac atcagcaccc tgggcaccta tacaatctac tggttctaca
       61 agcactggaa gctcatcgaa ccgcgcatgg aagagaagat catgccgttc tggcgcgcag
      121 ttttctttat tttctttgcg cactctctgt ttggccgcgt ggaaacggag gcaaggaagc
      181 gcagccttgc cctgtcctgg agcgcgggaa gtctggccgg aatctttgtg attctcaccg
      241 ttgcgcagcg aatcgtagac cggatctcca tgcgcagcca aacggtgggc tatgtggagg
      301 ctttgagcat cggcctgatg ttgtcctgca tctggccgat catagtcgtg cagaaaaccg
      361 ccaatcaggc gattttacgg atttgttggt tactcctgtg tcttttttga tttgctcagt
      421 gagcctaaaa atacgtatca gatttttaga aatcggagaa gcaaccattt cgcctaaata
      481 acgcgcctca attttcaatc cgttttcaag cgattgggaa tacccttcgc gtacagcgtc
      541 taatgctttt agcggagcgg ggtattggcc tttggtttct ttgagtacac ttttacgagc
      601 ctgactgtag atcagcggtt tgcctacgga ttcgagtaaa agattaatga gccccgatgg
      661 tttacgtcgt ttgacatacc cggttgtttt tccggaaata atttcttgta caaaagcggc
      721 ggcatagcgt tcggcaaatt cagacggtat aaccttgtct accacaccgt tcctcaatgc
      781 atttttcccg tcgtaagctt tacctgacaa aatcatgggg agggcacgct ggaggcctat
      841 aagacgaggc aggcgctggg taccgccaaa acccggcact atgccgatca tgacttccgg
      901 aaggccgatt tttgttttgg gtgaatcggt tgcaatacgg tacgtgcagg ccagcgctaa
      961 ctccgtacca cctcccatac acgcgccatg aataaggcaa acggtcggga agggaagctt
     1021 ttctatcttc gtgaaaatgg attgtccctg agcggcggca gcttcagcga ttatggggtc
     1081 ggtgatattt ttgatttcat caatatccgc accgacgata aaaataccgt ctttggcgct
     1141 gtgaatgata catccctgta tagatggcgc ctggcttgcc aaataatcca aagcctggtt
     1201 gagcgcttgc atgacggggg tggaaagttt attaaatttt tcaccgggat agtcaaaggt
     1261 cagccatgcc agaccgcctt cgatcttcag cttaagacta aataaggaat ccatattata
     1321 tatactcggg tgagcgttga aagttagaaa gccgatctgc gtatgaccgc aaatgcggct
     1381 gaaccgatgt tacggtactt cgactttatt caaaacttgc gtaatgattt cttcggtagt
     1441 cagttcctcg gcttcggcct cgtaagtgag tataacgcga tgacgcaata catccatagc
     1501 cacggcgcgt acgtcttccg ggatcacata accccgacgc ttaagaaatg catacgcttt
     1561 agatgcaagg gtcaaactga tggatgcgcg cggtgaagcg ccgaaatgaa tcagcggttt
     1621 taactccggc aatccatatt tttccggttg gcgtgtcgca aaaacgagat cgacaatata
     1681 gttttcgatt ttttcgtcca tgtatatttc gtttaccgtt ttgcgtgcgc gtaaaatttc
     1741 ttccggggtc gccaccggct taactttcag ttgcggtgcc gtcgttgcat tgcggcgtat
     1801 aatctccaat tcctcatcgc gcgtaggata acccaccttg agtttgagca taaagcgatc
     1861 cacctgcgct tcgggcagag gatatgttcc ttcttgctcg acgggatttt gcgtcgccag
     1921 tacgagaaaa ggttccggca gaggatacgt cgtatcgctg atggtaacct gacgctcttg
     1981 catagcttca agcaatgcgc tttgcacttt ggccggtgaa cggttgattt catccgctaa
     2041 aataagattg gaaaaaaccg gtcctttgcg ggtataaaaa tttccttctt tctgattgta
     2101 aatcagcgtt ccgagcaaat ccgcgggcaa aaggtcgggt gtgaactgaa tacgttgaaa
     2161 cttggtgtga atcgtttccg acaatgtttt gatcgctaag gttttggcaa gacccggtac
     2221 gccttcgagc agaacgtggc cgttggacaa aagcgctact aataaacgtt cgatcatata
     2281 tttttgaccg acgataacga ggccgacttc tttgagaatg ttgtcaatga atccgctttc
     2341 gatataaatg cgctcgttca gtgctttgat gtcaaccgat gccatgatgt ctcttgctcc
     2401 aaaactgtat ctttatacta atgtactttt ttcgatttta ataatcacga gtgtaatatc
     2461 gtcctgttgc atctcctgat ccggggcaaa tgtgctaacc gcggcgatga tttcgtcctg
     2521 aatattttta gcagaagttt cacggttatt ttctataacc tctttgagac gctcttcgcc
     2581 gaacaattct tcggcttcgt tgaatacttc cgtaatcccg tcagtgtaca atacgatgat
     2641 atcgcccgga tcaagcggct gggtcatctg acgataatcc tgatcgggga tgaagcccaa
     2701 aacaatgccg ccctcccgaa gtttgatcca ttcaccgctt tctttacgga tcatcggcgg
     2761 attatgtccc gcattcgtat aatacatgat gaggcgttca aaatcaatta tgcagtgaaa
     2821 gaacgttgca taccggcctt cggccgtatg tgtgcaaagg aggttgttca aactattaac
     2881 gacttcccag ctcgttttgt attcgtgcac catgccacga tagctggtgt acagtacgga
     2941 catcatgatg gcgcccggta cgccttttcc ggatacatcg ccgatggaaa ccgtgacctg
     3001 gtgatcatta agctttagca catcatagaa gtcgcctccg acagcataac agggaaggct
     3061 tttggccgtt atttgcagat tgggaatacg cggtaactgc tgcgggagga tagcctgctg
     3121 tatcttacct gcaattttaa gttctttttg caattcttca ttgcgaatgg cttgctcgtt
     3181 gagctgtgca ttttgtatgg cgattccggc ttcgccggcg atggcattga ggatatcgag
     3241 aaatccctca tggtaggcgc caagcatgtc actttcgaga ttgagcacac cgatggtgcg
     3301 gtcgctgtct ttgataggaa tagttatttc ggagcgagta ttgtcacgaa cactgacgta
     3361 gtgttcatca aaattgacgt cttttacgat gacgggtttg ccaatgatcg caacgtgacc
     3421 gatcagaccg gttccgactt tcatgcgaat ttgttccatc acttcaggat cgtaaccgcg
     3481 catgaccgcg ctttcgattt cttgcgtcac ttcattcacc aggaaaatac cggcggcatc
     3541 gtagggcacg atggatttga cggcatcgag aatattatcg agcaattttt cggtatgaat
     3601 ttcaccgtta aattttttgg cgatctctag aacgatttct ttttctacga gcgatttttg
     3661 aagttggctg tagaggcgcg tactttccag tgtattgccg acgcgttgca tggattgcca
     3721 gaggattttt ttatcttcgt cgctcagatc gtagcgaatc tgcgtctctt cacccaaaac
     3781 aagcaaggcg ctaatgccgt ctttttttaa catcggtatg atgtatttgc agccttcgtg
     3841 ttggaaaact tttaacgcat catcggttaa aacggtctga tgcataacaa acagttcatt
     3901 tattgtgaat acgcgtttgc cggataacaa ggcatcataa acggccatgt cggctttaat
     3961 acgtttggta atgagatcac tgcggccata cgaactcacc aatgaaaaaa attcagggat
     4021 gcgaaggtaa atcgcggcat atttgacatt cagcaatccg ctgatccgtt cggtgataat
     4081 atcggccatt tcatgataac ccatcgtgga aaccaaccgg gcatccaagt cacgaagatc
     4141 ttcctggcga cggtagtaat agctaaaaac gattttccgg atatagagtt ccgtgagttt
     4201 acggataggt tcataaatga ggatgaaaac cagagctttg aagaaaaaaa tcacatagtc
     4261 gctggtgcgc ggaaagtaac tgtcggagat atattctccg atgagaaaaa tggcgataaa
     4321 aatagcaacg aggacggcat ataagatgac atcacgaagc agcgtacgag aaaaattgaa
     4381 catgaaatcg taagatttga acgaaaatta aaaagcgggc taatatagcc gaccacgcgt
     4441 aaaaatacaa gcgtaatatc cgtctggcgg cttattttta atttgaattt agtgcaatga
     4501 atttggatat ttgatttata caaacaagct ttttatttat cacatatgtt attacaggaa
     4561 aggtaatccg ataatgtcag ctcatgatgc acaaagcggc aaacgccatg ttaccgagga
     4621 agaggctcgg gaagtagcag aatccgcccg cgagagcaaa tgggaaaacc ccagtttttt
     4681 gcgtgaaatt tttttaggta accttcgttt ggatttagtt catccgtatc cggaacccaa
     4741 aggaattaac cgtcccgagt ttcagcagtt ttataccgag ttcaagaagt ttatcgaaga
     4801 ggaagtggat tccgatgcga tcgatcgtga aggaaaaata cccatggccg tgcgcgaccg
     4861 gctcaaggcg atgggtgcgt tcggtatgaa gatcaaaaaa gaatacggtg gtttggaatt
     4921 ttcgcaagcc gagtataata aaataatgga attgatctgt acaaaagatg gcaatgtgat
     4981 tgccttatta tctgcacatc aatccatcgg cgtacctcaa ccgctgatgc tatttggtac
     5041 cgacgaacaa aaacaaaagt atctgcctcg attagccaaa ggcgaaattt cagcatttgc
     5101 gttgacggaa cctgaagccg gttcggaccc tgccaagttg cacaccaccg ctgttgaaag
     5161 cgaagacggt cagtcgtaca tactcaatgg tgaaaaacta tggtgtacca acggtacaat
     5221 cgctgatatc atcgttgtga tggcgcgcca tcctgaagac ggagcgatca ccgcgtttat
     5281 cgttgagacc aaatggcccg gtgtaaaagt agatcaccgc tgttacttca tgggtctcaa
     5341 agcattggaa aatgcggttc tgtcattcaa agacgtgcgc gtgcccaaag aaaacatcat
     5401 ttggggtaga ggtaaaggtc tcaagttggc gttaatcacg ctcaataccg ggcgattgac
     5461 gctaccggcg ggagcggcgg gaacggcgaa agcatgcctc catgtagcgc gggaatgggc
     5521 ttccaagcgt gtacagtggg gacaacccat cggtaaacat gaagccatcg cacaaaaaat
     5581 tgccgatatg gcatccatga cgtttgccat ggaatcggtt gcttcgttat ctacagcgat
     5641 ggcggataag aagctggata ttcgccttga ggccgctgtc ggtaaaatgt ataataccga
     5701 agccggttgg aaaattgtag atgatacgct gcagatacgc ggaggccgcg gatatgaaac
     5761 ggccgattct ttacgcgcac gcggtgaaga tccggtcgcc gttgaacgta tgatgcgtga
     5821 ttttcgaatt aatctgattt ttgaaggatc gagtgaaatc atgcgcttgt ttattgcgcg
     5881 tgaagccgta gataaacatt tggaagttgc ttggaaaatg attgatccca aatcctctat
     5941 cggtgaaaaa ctgggagcgc tgcctaagat tgcgctgttt tatggcattt ggtatccgac
     6001 acgttggtta ggttggggat ggtggccacg ttttagtgaa tttggtccgc tcgcgacgca
     6061 catgcgtttt gtcgaacgca aaacacgcaa attatcccgg gagattttcc acggtatgat
     6121 gcgccatcaa gctaaattgg catacaaaca agcattcttg tttcgcgccg ttgatatcgg
     6181 cgccgaactt tttgctatgt cggctacgat gtcgcgtgcc gtcaagatgg ccaaagaagg
     6241 taattccgat gcgatgaaac ttgcggatct tttttgtcgt aacgcacgac gtcgcgtcga
     6301 agtgtcattc aaagccatgt ggaataatga tgacaacgcc aaatacaaca tggccaaaga
     6361 tattttgaat aataaatacg gttggatgga agcgggtatc gcggaatcca aagaatcaaa
     6421 aactaaatca caatccgcac cgaccgtagc cgcttgaggc aatttataaa aatggcaata
     6481 aaaaaccctg agctcaactc agggttttat tttttcagta gaggaaaact atttattgac
     6541 gcgtaccgta ataccccagc ggtccggtaa attcggcgga tcatcgttcc aatcttcgta
     6601 ggaatgcgaa tca
//
LOCUS       DAOQGF010000155         6558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5768_length_6558_cov_6.254959, whole genome shotgun sequence.
ACCESSION   DAOQGF010000155 DAOQGF010000000
VERSION     DAOQGF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5768_length_6558_cov_6.254959"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..494
                     /locus_tag="PK937_14500"
     CDS             3..494
                     /locus_tag="PK937_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNH33833.1"
                     /translation="MDCVVACKTENQVPEGYNRDWITTEAKGKFPDVRLEIRSERCNH
                     CSNPPCVTCCPTGASHVHDYGGVVLVDHDVCIGCKACVASCPYDARFVHPEGYVDKCT
                     FCIHRVEKGLEPACVSVCPTHCMYFGDLDDPNSKVSQLLQKRAYHTNLPEAGTQPNIF
                     YLT"
     gene            502..1443
                     /gene="nrfD"
                     /locus_tag="PK937_14505"
     CDS             502..1443
                     /gene="nrfD"
                     /locus_tag="PK937_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNH33834.1"
                     /translation="MNELTHTRMNPLIDPALHIWGWEIPVYLFLGGLVAGFMILSGYF
                     TLKGHHKHDHFSSFYLPHVSLALLSLGMFSLFLDLEHKLYVWRLYTTFEITSPMSWGS
                     WILLLVYPSLLLNTLVRIPEAFSNRIHLFDKFSERIHAHPYLIKNIGVLNMIVGAILG
                     IYTGVLLSGLAARPLWNSSMLWILFLTSGISAAAAFVHLVTTDSVEREISAKADNAFL
                     IFELFVITLFIVGMLSSAQAQQTAARLILNGPYASVFWVFVVGCGIIIPLIIQLLAVN
                     HKIQHTPIAPIMVMLGGLILRFVIVYAGQASHWTIVH"
     gene            1461..2018
                     /locus_tag="PK937_14510"
     CDS             1461..2018
                     /locus_tag="PK937_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH33835.1"
                     /translation="MASSEEHFNEHRAVKPPQPYWNPYLSGIGLGLVLLASYLIMGRG
                     LGASGAFTTTTAVAVNAVAPEYAHQNTFYSEYLGDGTRSPLKDWLVFEVLGVFVGGLV
                     SGLLAGRIKKSVEKGPRISVRSRLLFAFAGGMLMGIGAKLARGCTSGQALTGGAVLNL
                     GSWAFMLMVFAGAYGLAYFLRRQWT"
     gene            2015..2614
                     /locus_tag="PK937_14515"
     CDS             2015..2614
                     /locus_tag="PK937_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH33836.1"
                     /translation="MNAPFFKYGLFGDEASLIVAFIIGIGFGFFLERAGFGSGVKLAA
                     QFYFYDMSVLKVMFSAIVTAMLGVYWFSWFGLLDLDLIYINPTFLLPQIVGGLLLGFG
                     FVIGGYCPGTSVVACSTGRVDAMVYVAGIFGGIFTFGFIFPYLESFYTATPMGAITLP
                     QWLNLPYGTVVFLVVVMAIGAFAAAEWGERKMAPRGGAS"
     gene            2611..3264
                     /locus_tag="PK937_14520"
     CDS             2611..3264
                     /locus_tag="PK937_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH33837.1"
                     /translation="MKNLWRDLTLTQKFGVVALMLGLLALIGGTPQRGSFYTIDSKAM
                     LMDVAKETDHIAVETLADWIIQGRSDYRLLDIRDAKAFETYHIPSAESVPLAELHQAE
                     LRRNEKIIFYSDGGIHASQAWFVLKARGYNGVYMLRGGLEEWQDRILYPHKPSTTDTE
                     SLRQFEKSAAVAKFFGGETQGEQSTAAVKSATTPLPKLPAGAKAAAPKTGKKKKEGC"
     gene            3531..5558
                     /locus_tag="PK937_14525"
     CDS             3531..5558
                     /locus_tag="PK937_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNH33838.1"
                     /translation="MRRLSNFILIMVLSLPLYSQDTLTLKNISYQPGQKLFVGLENED
                     LGAPLRVQLLNADGKPVSNRIVLFEIIERPSKSKGEKLGQAEAITNAEGMAETSFRLG
                     DHAGDYIIIARTTGMTGDTPHIIVRAQRTMWWMFLIFGLIGGLGIFLYGMNLGSGGLQ
                     KVAGDRLRTILGALTSNRFLGLLVGIAVTAIVQSSSATSVMVVGLVSSALMTLQQAIG
                     VLMGAHIGTTITVQLIAFNVSDYSLLLVGAGFLMTIATKRKFYIYMGEIILGFGFIFF
                     GMAVMSQAINPLKSMPAFIAWLIEFGNSPILGILASTVFTAIIQSSGATIGLCVVMAG
                     EGLLSLQSAMPLVFGANIGTCATALLSTIGATTDGKRTALAHTAFSVIGVFLFTPFLT
                     PFESLIQSITSWMGTEAVARQIANGHMIFNIMTAMILIGFVPLMTQLITKIIPEKAEG
                     DAFKPKYLNNAYLETPDIALQNAQREEVRLVTIVRAMYDNTMNLFSSENEEKVAKVRE
                     QLKQVNFLEGEIRRYLTRLSQKSISLTQSRRLMRLLLTIDDLRHMAERLGDSIANLAE
                     RYADNHVAFSPEGLEELKRFYGMIHERLDAVLTALEKEDAVMAEHIIKNKHMVSEEEV
                     RLRQLHMTRLQGGNEQLFVSSQYHFDLLTALQRVHIFCVKMAHEIVEQHNL"
     gene            complement(5770..>6558)
                     /locus_tag="PK937_14530"
     CDS             complement(5770..>6558)
                     /locus_tag="PK937_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33839.1"
                     /translation="TWRLVSSFGINKERLILTDKLTNWQTNLPTGHTLQFIPTPYCHF
                     RGAVMVYDIESRILFSGDLFGGLTGTDNYGLWADESHWDGMRAFHELYMPANKAIQFA
                     IDQIRKLDPAPVMIAPQHGSLIRGELIREFLDRMYSLKVGVDLLGRTEDDQLLGQYLE
                     VAKIVYKKAQAMVSNPDRILANFAGNAEIQELMTINDKDVVKIKKNASRALELFLYAL
                     SDHQSSRVSNQIKALMLQETMIRQLPPPTFAIEEAMDLGMESFT"
BASE COUNT         1591 a         1560 c         1601 g         1806 t
ORIGIN      
        1 gcatggattg tgtcgtggcc tgcaaaactg aaaatcaggt tcccgaagga tacaatcgcg
       61 actggatcac caccgaagcc aaaggaaaat ttcccgacgt gcgtctcgaa attcgatccg
      121 aacgttgtaa tcactgttcc aacccgccat gtgtgacctg ctgcccgacc ggcgccagtc
      181 atgtgcatga ctatggcggc gtcgtgctgg tcgatcatga tgtatgcatc ggctgcaagg
      241 catgcgtcgc atcatgtcct tacgatgcgc gttttgttca tcccgaaggt tatgtggata
      301 aatgtacatt ctgcattcat cgtgtggaaa agggattaga accggcttgc gtatctgttt
      361 gcccgacgca ttgtatgtac tttggcgatc tggatgaccc taatagtaaa gtatctcagc
      421 ttctgcagaa acgtgcttac catacgaatt taccggaagc cggtacgcaa ccgaatattt
      481 tttatctcac gtgaggatgc tatgaacgaa ttgacacata cgcgcatgaa tccgctgatt
      541 gatcccgcac tgcacatttg gggttgggaa atccccgtat atctttttct tggcggattg
      601 gtagcagggt ttatgatttt gtccggttat tttacgctca aaggccatca taaacacgat
      661 cacttttctt ctttttattt accgcatgtg agtttggctt tactgagcct cggtatgttt
      721 tccttatttc tagaccttga gcataagctt tatgtatggc gtctttacac gacatttgaa
      781 atcacttcgc cgatgtcctg gggatcttgg attttactgt tagtctatcc ttcgttactt
      841 ctcaatacgt tggtgcgcat tccggaggct tttagtaacc gcatacatct gtttgataaa
      901 ttttcggaac gcatccatgc gcatccttat ctcatcaaaa atatcggcgt gctcaatatg
      961 atcgtgggtg cgatattggg aatatacacg ggtgttctct tgagcggact ggccgcacga
     1021 ccgttgtgga atagctctat gctatggata ctttttctca cgtccggaat atccgccgcg
     1081 gccgccttcg ttcaccttgt cacgacagac tctgtcgagc gagaaatttc agcgaaagcg
     1141 gataatgcat ttttgatttt tgaattgttt gtaatcacgc tgttcatcgt aggtatgttg
     1201 agttccgcac aagcgcaaca aaccgcagca agactgatat taaacggtcc ttacgcttcg
     1261 gtattctggg tatttgtggt cggttgcggc atcatcattc ctttgatcat ccaactgctg
     1321 gcggtcaatc ataaaataca acatacgccg atagcgccga tcatggtgat gctgggcggg
     1381 ctcattttac gatttgttat cgtatatgcc gggcaggctt cacactggac tattgtacac
     1441 taatattcgg agagtaatct atggcatctt cagaagaaca ttttaatgaa catcgcgccg
     1501 tgaagccacc gcagccgtac tggaatccat atctgtccgg catcggcctc gggttggttt
     1561 tgctggcgtc gtatctgatc atggggcgtg gacttggcgc atcgggtgcg tttactacga
     1621 cgacggccgt tgctgtgaat gccgtagcgc cggagtatgc acaccaaaat acgttttaca
     1681 gcgaatacct cggtgacggc acacgcagcc cgctcaaaga ttggctggtt tttgaagtgc
     1741 tcggcgtttt tgtcggcgga ttggtttccg ggcttctagc cgggcgcatc aaaaaatccg
     1801 tcgaaaaagg cccgcgcatt tccgtccgat cacgcctctt atttgctttt gcaggcggca
     1861 tgctgatggg catcggtgca aaacttgccc ggggttgcac gagcggacaa gccttgaccg
     1921 gcggcgccgt acttaatctc ggaagctggg catttatgct gatggtattc gccggcgctt
     1981 acggactggc ttattttttg aggaggcaat ggacatgaac gcaccttttt tcaaatacgg
     2041 attattcggt gacgaagcga gcctgatcgt cgcttttatc atcggtatcg ggtttggttt
     2101 ttttcttgaa cgcgccggat ttggcagcgg tgtaaaactc gccgcacagt tttattttta
     2161 tgatatgagc gtactcaaag tcatgtttag tgcaatcgta acggccatgc tgggcgtata
     2221 ttggttctca tggtttggct tgcttgatct cgatctgatt tatatcaatc ccacatttct
     2281 tctcccgcaa atcgtcggag gtcttctgct tgggttcgga tttgtgatcg gcggatattg
     2341 tccgggcact tcggtcgtgg cgtgttcgac aggacgcgtg gacgcgatgg tttatgtggc
     2401 cggtattttc ggaggaattt ttacgttcgg atttattttt ccttacctcg aatcgtttta
     2461 tacagcgacg ccgatgggcg cgatcactct gccgcaatgg ctcaatctgc cgtacggcac
     2521 cgtcgtattt cttgtcgttg tcatggcgat cggcgcattt gccgccgccg agtggggcga
     2581 gagaaaaatg gctccccgag gaggtgcatc gtgaaaaacc tatggcgtga tctcacgtta
     2641 acgcagaagt tcggcgtcgt cgcgctgatg ctcggcttat tggcactgat cggcggtaca
     2701 ccgcaacgcg gttcttttta tacgatagac agcaaagcca tgctgatgga cgtcgccaaa
     2761 gagaccgatc acatcgccgt ggaaacattg gccgactgga tcatccaagg ccgttcggat
     2821 tacagactgc tggatatccg cgacgccaaa gcgttcgaga cgtaccacat tccttcagcc
     2881 gaatccgtcc cgctcgccga actccatcag gccgaattgc gccgtaatga aaaaattatt
     2941 ttttactccg atggcggtat tcatgcttcg caggcgtggt ttgtactcaa agcccgcggc
     3001 tacaacggag tatatatgct gcggggcgga ctggaagaat ggcaggatcg tattttgtat
     3061 ccgcacaaac cgtcaacaac cgatacggaa tccctgaggc agtttgaaaa gtccgcggcg
     3121 gttgccaaat tttttggcgg cgaaacacaa ggcgaacaat ctacagcggc cgtcaaatca
     3181 gcgacgacac cgctgcccaa actccctgcg ggcgctaaag ccgccgcacc taaaaccgga
     3241 aaaaagaaaa aagaagggtg ctgatcaaat ttatatgact tttcgcatgg ctaaatcccc
     3301 tacgggatag gatatttaca aaaacgtgcg ggctgacgat atgcccatag attgatacat
     3361 atgattttgt aatacaaaga cgttcctcaa agaacgtctt ttcttttgga tacggtgaaa
     3421 accgtttgca acttctgttt ttgtttgtga aatttaacac gcatgtatct tcatattttt
     3481 tacctacgct gaacggccgc caggccgtct tcaggtttcg gagaatcatc atgagacgcc
     3541 tttcgaactt tattttgatt atggtcttga gcctaccgct atattctcaa gacacgctga
     3601 cattaaaaaa tatttcttac caacccggtc agaaattatt tgtcggactt gaaaacgaag
     3661 accttggagc gccgttgcgt gtccaattgc tcaacgccga cgggaaaccc gtaagtaacc
     3721 ggatcgtcct atttgagatc atcgaacggc cttcaaaatc taaaggcgaa aaactcgggc
     3781 aagctgaagc gataaccaat gccgaaggta tggccgaaac aagttttcgt ctcggtgatc
     3841 atgcgggtga ttatatcatc attgcccgca cgaccggcat gaccggtgat acaccgcata
     3901 tcatagttcg cgcccaacgc acgatgtggt ggatgttttt gattttcgga ttgatcggcg
     3961 gcttgggcat atttttgtac gggatgaatt tgggttccgg cggtttacaa aaagtagccg
     4021 gcgatcgtct acgtactatt ctcggcgcat tgaccagtaa ccgttttttg ggattgcttg
     4081 tcggtattgc cgtgaccgcc atagtgcaaa gcagttcggc cacatcggtg atggttgtcg
     4141 gattggtcag ttccgctttg atgacattac aacaagccat cggcgtactc atgggcgccc
     4201 atatcggcac tacgatcacc gtacaattga tagccttcaa cgtttccgat tattctttgt
     4261 tgctggttgg cgccggattt cttatgacga ttgcaacgaa acgaaaattt tacatctata
     4321 tgggtgaaat catcctcgga ttcggtttta tatttttcgg aatggccgtg atgtcgcagg
     4381 ccattaatcc gttaaaatcc atgccggctt tcatcgcgtg gctgatcgaa tttggaaatt
     4441 ctccgattct cggtattctc gcctccaccg tgtttacggc cattattcaa tccagcggcg
     4501 cgacgatcgg tttgtgtgtc gtcatggccg gagaagggct gctttcttta caatccgcca
     4561 tgccgcttgt atttggcgcc aatatcggta catgcgcaac ggctttgctt tctaccatcg
     4621 gtgcgacgac cgacggcaaa cgtacggcct tggcccatac ggcattctcc gtgatcggcg
     4681 tatttttatt cacgccattc ctcaccccgt tcgaatcact catccagagt attacctcct
     4741 ggatgggtac cgaagccgtg gcgcgtcaga ttgccaacgg gcacatgata ttcaatatca
     4801 tgacggcgat gatcctgatc ggatttgtac cactcatgac tcaattgatc accaaaatta
     4861 ttcccgaaaa agccgaaggg gatgcgttca aaccgaaata tcttaacaac gcctatctgg
     4921 aaactccgga cattgctttg cagaatgctc agcgtgaaga agtgcggttg gtcactatcg
     4981 tacgtgccat gtatgacaac accatgaatc ttttttcctc agaaaacgag gaaaaagtgg
     5041 ccaaagtgcg cgaacagttg aaacaagtga atttcctcga gggtgaaatc cggcgttatc
     5101 tgacgcgttt atcgcaaaag agtatcagtt tgacgcagag ccggcgactt atgcggcttc
     5161 tgctcaccat tgatgatttg cggcatatgg ccgaacgcct tggtgacagc atcgccaatc
     5221 tggccgaacg ttatgcggat aatcacgtgg cgttttctcc ggaagggctg gaagagctaa
     5281 aacgtttcta cggtatgata catgaacggc ttgacgcggt actcacagca cttgaaaaag
     5341 aagacgccgt catggccgaa catatcatca aaaacaaaca tatggtttcc gaagaggaag
     5401 tgcggttgcg tcagttgcac atgacacgcc ttcaaggcgg taacgaacaa ctatttgtca
     5461 gcagtcaata tcactttgat ttgcttacgg cattacaacg agttcatatt ttctgcgtca
     5521 agatggcgca tgaaatagta gaacagcata atttataacg gatattgttt agatataaaa
     5581 aacctccgat tgctcggagg ttttttatat taaggatcgc ataatcatca aaacaccgcc
     5641 ggcgaatcag aactttgtca ctacacgaat gaaaattcaa ttacaagacc attgagaatt
     5701 cgttacgcca ttgcgttata gcgagaacgc atccttctcg caagccgcaa agaaaaatgg
     5761 aaaaatattt tacgtaaaac tttccattcc aagatccatg gcttcttcga tcgcaaaagt
     5821 cggcggcgga agttggcgta tcatggtttc ttgtagcatc agcgctttga tttgattgga
     5881 aacgcgcgac gactgatgat cactgagcgc gtacagaaaa agttctaacg cacggcttgc
     5941 attctttttg atcttcacta cgtctttatc gttgatcgtc atgagttcct ggatttcggc
     6001 attgcccgca aaattggcta atattcgatc cgggttgctg accatagctt gggccttttt
     6061 atatacgatc ttggctactt cgaggtattg ccccaataat tgatcatctt ccgtacgccc
     6121 caaaagatca acaccgactt tgagtgaata catacgatct agaaattcgc gtatcaattc
     6181 gccgcgaatc aacgacccgt gttgcggagc gatcatcacc ggcgccggat caagcttgcg
     6241 tatctgatca atggcgaatt gaatcgcttt attggccggc atgtagagtt cgtgaaatgc
     6301 gcgcattccg tcccaatggc tttcatcggc ccacaatccg taattgtccg tgccggtaag
     6361 gcctccgaat aaatcaccgg aaaaaagaat gcgggattcg atatcataca ccatcacggc
     6421 gccgcgaaaa tgacaatacg gcgtcggaat aaattgtaac gtatgcccgg tcggaagatt
     6481 ggtctgccag ttggtcaatt tatcggtaag aatcaaacgt tctttattaa taccaaagct
     6541 ggaaacgagc cgccatgt
//
LOCUS       DAOQGF010000156         6498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5851_length_6498_cov_5.983393, whole genome shotgun sequence.
ACCESSION   DAOQGF010000156 DAOQGF010000000
VERSION     DAOQGF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5851_length_6498_cov_5.983393"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PK937_14535"
     CDS             <1..1583
                     /locus_tag="PK937_14535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33840.1"
                     /translation="ILDVTAPTISNVTLPASVASGASLSVTATVTDNGSLSGVKLFFQ
                     KGTSAYDSLTMTAGTNNTFTATVHDSNITTEGIALYIRARDSRVNTAISDTSSVPVTF
                     TTVASSIAGSKYPTGIPSNRWRLVSVPADLDSKNLSSILSGVYSGDYIAYNAQPAEIT
                     ALANGAGMWFYHKSPVDMPINTAAGKTNLTTGFDMSLAPGWNLIGNPYPFTINFAALE
                     DTAFYGPIEYGVGATEAWSTEVTTLQPFGGYAIYVKGNAARTIRVTPDNFVWMPKSSN
                     QLEGEKFRIMLSAEGLKKQIRYGDLYTTLAVLDRDDAEAFAQPEPKNPGQTIGVYFPS
                     GDHGLNRKTIRFDKQGITSDFVLESGLESGELTMQIVNNEPGWSVRLFNVSTNSFIDH
                     ETAMSLPPSGTKWLMRILAGTPEYLAGKQTELEQLPKAFELLQNYPNPFNPGTTIRYA
                     LASQAKVTLTVYNMLGQKVRTLVNGDRQETGLRTAVWDGRDERGALVSSGVYLYRVEL
                     ETTTGSRKSFTRKMLLVK"
     gene            1599..2834
                     /locus_tag="PK937_14540"
     CDS             1599..2834
                     /locus_tag="PK937_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33841.1"
                     /translation="MMRKNFYTMLMATLTTMSGLISCGTTRNSMLQDNPELIIALGGS
                     SYTQLSDADKYDAAEYLVRKGQYENARVIFEDIITHNPGVVAVKYKLAQLYLKLDSVT
                     FAEKTPKGDTYYFTKNGAELGEAVLREIYEKDPWFLPAYSELMMLEAKRQNYDGVKAI
                     YEKAITKHKDFSTSDYRVGFLTMGNPDLADRFSEGMRMMEKGKKTYTDLYESYKSLGN
                     IFNVQHKDTVAYNQLIRAISYRTEAIDIFSPYYHLADVCETLYQVKKEEHYKTMALQY
                     ACASLHYFPGYEPSVNVIRRLTGVAKDTSADSSKTYRAAAEAFCAGSTLPADDSVTIA
                     SGYTTVIPHSYLTEEMEAKQADIKAREIKRSRRPLLIAGGIAGGALAVLLLLSLVGGG
                     GGSSSGPLGGGPPDFPTPQ"
     gene            3159..3965
                     /locus_tag="PK937_14545"
     CDS             3159..3965
                     /locus_tag="PK937_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33842.1"
                     /translation="MISYLRSIGLSNPSQNAFLFDNQLPYRQSPAKVFCKIGRVVVAM
                     LMIGSNTIFAQLINMSGVVSSATDDAAIYTVSKGRFTNRISIGENCNANIVCNDERSL
                     PEHICARRSDGSGSVLVMSNRDAITSVAGQQTFPAMGEEIFGGVIIAWTDEWHDTFDQ
                     VIYTQCIEASCRFGISTVPASSASFEVITKIQEPLHWNEVSMACQYPVPDDMCCSQAF
                     TFHSSHFLEPLFYHATGTGICRSAPHQRSTYFLAFFHGRNVDIDFDIIKY"
     gene            4089..>6498
                     /locus_tag="PK937_14550"
     CDS             4089..>6498
                     /locus_tag="PK937_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNH33843.1"
                     /translation="MKLARVLVKITMAVAVMGLTGIGSVSAQWSTNPTVSNPVSTTFG
                     DQRYPQAIPDGSGGVFIVWEDYRGGANPDIYAQRLNATGAPLWTVDGVVISNATSGQS
                     SPKIVTDNNGGAIITWTDNRNGGANYDIYAQRINSSGTVLWTANGVQITGASGNQTDA
                     RIAADGSGGAVIAWNDNRGGATDIYVQKVNATGVPQWTVDGVSICVATDNQTIGGITD
                     DGSGGAIITWHDFRNGTDNRIYAQRINNVGVTQWTVDGEAISTFASYQDYPSIVADGS
                     GGAFIAWRDTRNGDFDLFAQRVSSAGALQWGVNGVAVAVADGFQQIPSLVADGSGGVF
                     IGWYDTRSAGAGIYAQRLNSSGVALWPANGVAASTNGSPDEAVITSDGAGGIFVSWYA
                     TPAIDADIFAQRIDGSGNILWSPNGNVVCSANGYQFWHTMVHDGSGNAIVIWSDQRSG
                     NYDVYAQLMTSQGDLGVNNPYFTVASTVPGQNARNISSNSGITVTFSQVVNVGTLTAS
                     NMKVYGSQSGLHSASYTPSGLTVTINPDVDFKPGEAVSVILTSGIRATDTDTLKTYVT
                     QFTVASDIAPATFPQVASNFGVGTNPRSVYAADFDGDGDMDIATANGGSSNVSIRLND
                     GAGNFSGSTDVAVASGAFSVYAADFDGDGDVDVATANPSSNSVSIRLNDGAGNFSGST
                     NVGVGTGPQSVYVADFDGDGDMDIAVANTGSSTVSIRMNDGAGNFSGTTDVPSGSVPY
                     SVYAADLDGDGDIDIATANFSSNDVSIRLNDGSGNFSGTTDIVVETNPQSVYAADFDG
                     DGDVD"
BASE COUNT         1671 a         1473 c         1710 g         1644 t
ORIGIN      
        1 atattttgga tgtgaccgca ccgacgatta gcaatgttac ccttccggca tccgttgcat
       61 cgggtgcatc gctatcggtg acagcgacgg ttacggataa tggcagtcta tcgggggtaa
      121 aactattttt ccaaaaaggc acatcggctt atgattcact gaccatgaca gccggtacga
      181 ataatacatt taccgctacc gtgcatgatt cgaatatcac gacggaaggt attgctttgt
      241 atattcgtgc gcgggatagc agagttaata cagccatcag tgatacaagt agcgtaccgg
      301 taacatttac gaccgtggcc tcatccatcg caggatctaa atatcctacg ggcattccat
      361 ccaatcgttg gcgtttggtg tctgtaccgg cagatctgga tagcaaaaat ttgagttcga
      421 ttttaagcgg tgtgtattcg ggtgactata tcgcgtacaa cgcgcaacct gcagaaatca
      481 cagccctggc caatggtgca gggatgtggt tttatcacaa gtcgccggta gatatgccga
      541 ttaatacggc agcgggtaaa accaatctga cgacgggatt tgatatgtca ctggccccgg
      601 gatggaatct gatcggaaac ccatacccgt tcacgattaa ttttgccgca cttgaagata
      661 cggcgtttta cgggccgatc gaatatggcg tcggcgcgac cgaagcatgg agcactgaag
      721 tcaccacgct gcagcctttt ggcgggtatg cgatttatgt aaaaggtaat gcggcgcgta
      781 cgattcgtgt aacaccggat aattttgttt ggatgcctaa atcatcgaac caattagaag
      841 gtgaaaaatt tcgtatcatg ctcagcgccg aaggtttgaa aaaacaaatt cgctacggtg
      901 atctttatac tacgctggct gtattagatc gcgatgatgc ggaagcgttt gcacagccgg
      961 agcctaagaa cccgggacaa acaatcggcg tgtatttccc gtcgggcgat catggtctca
     1021 atcgcaaaac gatacgtttt gataagcagg gtattacctc ggactttgta ttggaatccg
     1081 gtttggagtc gggtgaacta acgatgcaga ttgtcaataa tgaaccgggt tggtccgtgc
     1141 gtttattcaa tgtcagcacg aattcattta ttgatcatga aacggcaatg tccttaccgc
     1201 cgtcgggcac aaaatggctg atgcgtattt tggcgggaac tccggagtat cttgcaggta
     1261 aacaaacgga attggaacaa cttcctaaag cgtttgaatt gctgcaaaac taccccaacc
     1321 ctttcaatcc agggacgacg atacgttatg cactggcttc gcaggctaaa gtgacgctga
     1381 cggtttataa tatgcttgga caaaaagtgc gaacattggt caacggcgac cgccaagaga
     1441 ccggtttgcg tacggcggta tgggacgggc gtgacgaacg tggcgcttta gtttcatccg
     1501 gtgtttatct atatcgcgtg gaattggaaa caacaaccgg ttcgcgtaaa tcattcaccc
     1561 gaaaaatgct tttggttaaa taaggatata gcaatcgtat gatgcgtaaa aatttttaca
     1621 cgatgttgat ggcgactctg acgacgatgt cagggttgat ttcctgcggt acgacgcgca
     1681 attccatgct tcaggataat ccggaactga tcattgcgct cggcggatcc agttacacgc
     1741 aactttccga tgccgataaa tatgatgccg cggagtatct ggtgcgcaaa gggcaatacg
     1801 aaaacgcacg ggttattttt gaagatatca ttacgcataa tcccggtgtc gtggccgtaa
     1861 aatataaatt ggcgcagttg tatctcaaat tggacagcgt gacgtttgcg gaaaaaacgc
     1921 ccaaaggcga tacgtattat tttaccaaaa acggcgcgga actcggcgaa gccgtactcc
     1981 gtgaaatcta tgaaaaagat ccgtggtttt tgcctgcgta ttccgagctg atgatgcttg
     2041 aagccaagcg tcaaaattat gatggggtca aagcgattta tgaaaaagcc ataacgaaac
     2101 ataaagattt ttctacgtcc gattatcgcg tcggatttct gacgatgggg aatccggatt
     2161 tggctgatcg tttcagcgaa ggtatgcgta tgatggaaaa aggaaaaaag acgtacaccg
     2221 atttgtatga atcctacaaa agccttggga atatctttaa tgtacaacac aaagataccg
     2281 tagcttacaa tcaactgata cgtgcgatca gctaccgtac ggaagcgata gacattttta
     2341 gcccgtatta ccatttggcc gatgtctgcg aaacgctgta tcaagtaaaa aaagaagagc
     2401 attataaaac gatggcgctt caatacgctt gtgccagtct tcactatttc cccggatatg
     2461 aaccgtcggt caacgtaatt cgacgcttga caggtgtggc taaagatact tccgccgatt
     2521 ccagtaaaac ataccgcgcc gctgcggagg cattctgtgc cggcagtaca ttgcctgcgg
     2581 atgacagtgt gactatcgcg tcgggataca cgacggtgat cccgcacagt tatctgacgg
     2641 aagaaatgga agccaaacaa gctgatatca aagcccgcga aatcaagcgt tcccgtcgtc
     2701 cgctattaat agccggcgga atcgcaggcg gtgcattggc cgtgttactg ctgctgagtt
     2761 tggttggcgg tggcggcggt agttcgtccg gtccgctcgg aggtggtccc ccggacttcc
     2821 caacgccgca gtaaaaaaga agttttgcga tcccatattc atggggtcgg caaagggctt
     2881 gacactgcgc agcctttgcg gtaatttggt tcaaccgttt gattatcttg tggtaaaggt
     2941 cacgactgat aagatagagt ttcggtattc tcattcggac gtattttgag gaaaacacgt
     3001 cctttacaat cccatgagcg ggcgctgttc tcagcctgca aaaacgaaat ttgacgtttc
     3061 tttcgatata cacgcttcgt gcgttttgta tattgcgttt ttatagtctg cgcgattaga
     3121 gaaacggtta tataatatcc acatctatgg agatatccgt gatctcgtat ttacgctcga
     3181 tcggtctttc caacccatcg caaaacgcat ttctttttga caaccaatta ccttatcggc
     3241 aatcacccgc aaaggtcttt tgtaagatag gccgtgttgt agttgcgatg ctcatgatcg
     3301 gaagcaatac gatttttgca caactgataa acatgtcagg tgttgtatca agtgcgaccg
     3361 atgacgcggc tatctatacg gtatcgaaag gccgatttac caatagaatt agcatcggcg
     3421 aaaattgcaa cgctaatatt gtctgcaatg acgagagatc attgccggag cacatatgtg
     3481 cccgacggag tgacggctcg ggatcagttc tggtgatgag caatcgtgat gcaatcactt
     3541 cggttgcggg ccagcaaacc ttcccggcca tgggcgagga aatttttggc ggtgtgatta
     3601 tcgcatggac ggatgaatgg cacgatacat ttgatcaggt tatttatacg cagtgtatcg
     3661 aagcatcatg tcgttttggc atatccacgg ttccagcttc gtccgcttca ttcgaggtga
     3721 ttacgaaaat tcaagagcca ttgcactgga atgaagtttc catggcctgc caatatcccg
     3781 tccctgatga tatgtgttgt tcccaagctt ttacatttca ttcttctcat tttttggagc
     3841 ctctgtttta ccacgcgacc ggaactggga tttgccgcag tgcgccgcat caaaggagta
     3901 catatttttt agcgtttttt cacggtagaa atgttgacat tgattttgac ataattaagt
     3961 attagctcgc atattccatt ctttcattat atcggagttc atccatgaac ggattgccac
     4021 gactttatga attggtttta tcaattcgcc atgatattcc aaatctattc cgtttccacg
     4081 tttcgaggat gaaacttgca cgcgtgttgg tcaaaataac catggccgta gcggtcatgg
     4141 gtcttaccgg catcggaagt gtgtcggccc aatggtcaac gaatccaaca gttagtaatc
     4201 ccgtatctac gacatttggg gatcaaagat accctcaggc tattccagac ggcagcggcg
     4261 gggttttcat tgtatgggaa gattaccgcg gcggtgccaa tcccgatatc tacgcccaac
     4321 gattgaacgc aaccggcgcg cctctctgga cagttgacgg cgtggtcatc agtaatgcga
     4381 ccagcgggca gtcaagtccg aagatcgtaa ccgataacaa cggcggtgcc attataacgt
     4441 ggacggataa ccggaacggc ggtgctaatt acgacatata tgcgcaacgg atcaactcaa
     4501 gcggcactgt actttggacg gcgaacggag tgcagataac cggcgcgagc ggcaatcaaa
     4561 cggatgcacg aattgccgcc gacggctcgg gcggagccgt aattgcatgg aacgacaata
     4621 ggggcggtgc aacagatata tatgtacaaa aggtcaacgc gactggggtt cctcaatgga
     4681 cggtcgatgg cgtttccatt tgcgtcgcaa ctgacaacca aaccattggc gggataaccg
     4741 acgacggctc gggcggcgcg atcatcacat ggcatgattt tcgaaacggc acggataatc
     4801 ggatctatgc acaaaggatc aacaatgtcg gtgtcacgca atggacggtt gacggtgaag
     4861 ctatcagtac ctttgcaagt tatcaagact acccttcaat tgtcgccgac ggatcaggtg
     4921 gagcctttat cgcatggcgg gatacgcgta atggagattt cgatctcttt gcccagcgtg
     4981 taagcagcgc aggcgctctg cagtggggcg taaacggcgt tgccgttgct gttgcggacg
     5041 gatttcaaca gatcccaagt cttgtagcgg acggatcagg cggagtattt ataggatggt
     5101 atgacacccg aagcgccggc gcggggatat atgcgcaaag acttaactct tccggcgtcg
     5161 ctctgtggcc ggccaatggt gtggccgcca gcacgaacgg ttctcccgat gaagccgtta
     5221 tcacatccga cggcgcaggc gggatatttg tttcatggta tgcgaccccc gccatcgacg
     5281 cagacatttt tgcccagcgt atagacggat ccggaaatat tctttggtcg ccaaacggca
     5341 atgttgtttg ttctgcaaac ggttatcaat tctggcatac gatggtacac gacggctccg
     5401 ggaatgcgat cgttatttgg tcggatcaga ggagcggaaa ttatgacgtt tatgcccaat
     5461 tgatgacttc gcagggcgat cttggcgtca acaatccgta tttcacggtg gcctccacgg
     5521 tgccgggaca gaacgcacga aacatcagtt caaacagcgg catcacagtg actttctcac
     5581 aagtcgtcaa tgttggtaca ttgacagctt cgaatatgaa agtatacggt tcacagagcg
     5641 gacttcattc tgcttcatac acacccagcg gcttaacggt gacgatcaat ccggatgttg
     5701 atttcaaacc cggtgaagcc gtttcggtta ttctgacaag cggtatcagg gccacggata
     5761 cggatactct gaaaacgtac gtcacgcaat tcacagttgc atctgatatt gcgccggcaa
     5821 cattcccgca ggtagctagc aacttcgggg tagggaccaa tccacgatcc gtctatgcgg
     5881 cggactttga cggggatggg gatatggata tcgcgacggc aaacgggggt tcaagtaatg
     5941 tttcgatacg cctgaatgac ggagcaggta atttttcggg aagcaccgat gtcgcggttg
     6001 cgtcaggcgc attttcagtg tatgccgcag attttgacgg cgatggggac gtggacgtcg
     6061 cgacagcaaa tccaagttca aatagtgttt cgatacgcct gaatgacggc gctggaaatt
     6121 tttccggaag cacgaatgtc ggggttggca caggaccaca atccgtctat gtggcggatt
     6181 ttgatggcga cggcgacatg gacatagcgg tggcaaacac aggttcgagt acggtatcca
     6241 ttcgaatgaa tgatggtgcg ggtaattttt cggggaccac tgatgttccg tctgggtcgg
     6301 taccgtattc agtttatgcg gcagatcttg acggcgacgg agacatagat atcgcgacgg
     6361 ctaacttcag ctcaaatgat gtttcgatac gcctaaatga cgggtcaggg aacttttcag
     6421 ggacgaccga tatcgtggtg gaaacaaatc cacaatcagt gtatgccgca gattttgacg
     6481 gcgatgggga cgtggaca
//
LOCUS       DAOQGF010000157         6484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5871_length_6484_cov_6.758438, whole genome shotgun sequence.
ACCESSION   DAOQGF010000157 DAOQGF010000000
VERSION     DAOQGF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_6484_cov_6.758438"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PK937_14555"
     CDS             <1..1412
                     /locus_tag="PK937_14555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH33844.1"
                     /translation="SHIRILVVDDEKAARYGMLKALKPEGYKLSEADDGLSALNLLAN
                     NPFDIVITDVSMAGMNGMDLLKKIKERTPHCFVIVITAHGSERLAVEAIKSGAFNYLA
                     KPYDIEELRALVSHAAQQIAVVHENERLRAELKEAKGFGQFVGSSKPMRQVFDLLERV
                     SRNNITVLIRGESGTGKELVARTIHEKSNRRSGPFISINCAALPKELIESELFGYEKG
                     AFTGAISSKQGRFELADGGTLFLDEIGDMSLETQAKVLRVIQERKFERVGGTESISVD
                     VRILSATHRDLARFIRDGQFREDLYYRINVVEIELPPLRSRSEDIHLLVNHYLVMFAQ
                     KHKAQVTRFSQDALKAMLEYDWPGNVRELINVVERCVVLSTEEEIRRDILPSEIIGGG
                     RPTTLGVRDMLEKGDINFQDAKHRVVQTFEREFFMEAVRMNNWNISQTANKLGMKRQY
                     LQQKLKELELNIQEAKENI"
     gene            1409..2293
                     /gene="murI"
                     /locus_tag="PK937_14560"
     CDS             1409..2293
                     /gene="murI"
                     /locus_tag="PK937_14560"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNH33845.1"
                     /translation="MMRSQTPEPSVLPAIQSAPPASEQPIGIFDSGIGGLTVVKEVMN
                     QLPYERIIYLGDTARVPYGTKSDRTIKSFTLQSCLFLLEHNVKMIIIACNTASAVALD
                     FLSNMFKIPVIGVIVPGSEAAVRKSLRKKIGVIGTHTTVNSQAYKKTIHALDAQCEVF
                     SQPCSLFVPLAEEGWLRHDVTQRIAAEYLNVFSDKDIDTLVMGCTHYPILSGAIQEAM
                     NTIMRRPVTLIDSGVETAKRTRQILDEKKLLNTSGQKPYHRFYVTDLPKNFMRVGEMF
                     LGSKIDSVETVNVEVMGF"
     gene            complement(2365..2766)
                     /locus_tag="PK937_14565"
     CDS             complement(2365..2766)
                     /locus_tag="PK937_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33846.1"
                     /translation="MKTNFLTSLHPKILYAGLVLSSLIGYLEWGSGHYTFLVQAEFEI
                     IRKFFTHTDAITHPMIALPLLGQALLLVTLFQNKPRPVIVLTGLLGLGVLLWFIFLIG
                     LISLNLKITFAALPFLIVSIIVLYSMRKGGK"
     gene            complement(2732..>3156)
                     /locus_tag="PK937_14570"
     CDS             complement(2732..>3156)
                     /locus_tag="PK937_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33847.1"
                     /translation="LVIHTSPENIWRVWMAVDEWPLWTDTILSIKKLSPGSLQTGSRV
                     LIEQPKLAIAVWKITEFEPVKQFTWSKTNLLLRIDAIHALELTSDGCIVTLVIKFSGL
                     FANLINKKTCALVDSYLVRESQNLKQFCENKFSNVAAS"
     assembly_gap    3157..3256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3329..4729)
                     /locus_tag="PK937_14575"
     CDS             complement(3329..4729)
                     /locus_tag="PK937_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNH33848.1"
                     /translation="MLFILGVLAVMGTNDFDAYFTGQTMRFDYYHSGTAKEEHISPDE
                     YRIEGVWPGSKTVLLDETGLGKYLFEVSDVASGKLLYTRGFASIYGEWETTGEAAKGV
                     WRSFHESQRFPEPKNAVKLTLKKRGADNRFASFYETTIDPKSRFVNRAPAVKNGEVWS
                     VFENGDPSQKVDLLILSDGYTAGEKNKFHADVERLVKVMFETEPFKSRKKDFNVRAMD
                     VASAQSGISNPRKDIWRKSALGLSFNSFDSDRYVLTYENKSLRETAANAPYDALILLA
                     NDRKYGGGGIFNLWATVTADAEPASYVFVHEFGHSFAGLGDEYYTSPVSYENFTAPST
                     EPSEPNITALLDPKNLKWKHLVSKDTPLPTPWNKTAYDTYEIEMQKKRAKLIEEKASE
                     ETMEALFREVKTKTTPMLNTDKYFGKVGAFEGAGYEAKGLYRPELDCIMFTRNNVPFC
                     KVCHEAIERIIDLYAK"
     gene            complement(4821..>6484)
                     /locus_tag="PK937_14580"
     CDS             complement(4821..>6484)
                     /locus_tag="PK937_14580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNH33849.1"
                     /translation="LDEIDPITDKTALVKLLGRRLRADADWLTYRSPGLLGLSVEVGY
                     FGEKTYVPFLMQGGLGLEERSYYLSEDAANKKIRATYQNYISQQFKQAGFSHPERRAQ
                     SVLGIEILLAKTHASDEFSNNDRNGSHRWSRNDFERRAPGMDWSVFFEEAGLKHQESF
                     VVWQPDAITGLASLIAAQPVEVWKDYLSFQAIHAYADVLPSAFAEAALTLRSSIQDAP
                     SPARSQRALDATQKFLGEAVGWLYVERYFPASQKSRVQAIATNVIASLRERVKTVSWM
                     SDESKTQAMVKLQTLYFGLGYPNVWQDYSDLNIDPANPVKNLQNIADRNYRRALARLG
                     KPPDTTEWATTPHTAGALLLFQYNACNFAAALLQAPKFDSTLSDAANYGAIGAIVGHE
                     SSHFIDPLGAEWNAERELKHWWTPNDMKKFQSAAKPLVEQFSGYRPFDDLAVNGELTR
                     SENIADLAGLAAAFDAYRKTLGDKIQNQDYVRQQDREFFIGFAQSWRSKTTEKGLRVQ
                     IATDHHAPEQYRIATVRNLDAWYDAFDVKPGDKLYLTPESRVRIW"
BASE COUNT         1677 a         1498 c         1552 g         1657 t
ORIGIN      
        1 catcgcatat ccgcattctc gtcgtggatg atgaaaaagc ggcgcgttat ggtatgctca
       61 aagcgctcaa acccgaaggt tacaaactat cggaagccga cgacggatta tcggcgctca
      121 atcttttggc caataatccc tttgatatcg tcattacgga tgtatccatg gccggcatga
      181 acggtatgga tttgctgaaa aaaataaaag aacgcacgcc gcattgtttt gttatcgtga
      241 tcacggcgca cgggtcggaa cgtcttgccg tcgaggctat caaatccggc gcgtttaatt
      301 atttggccaa accctacgat atcgaagaac tgcgggcact cgtgtcgcat gcggcacaac
      361 aaatcgccgt cgtgcatgaa aacgaacgat tacgcgccga gctcaaagaa gccaaaggtt
      421 tcgggcaatt tgtcggcagc agcaaaccca tgcgtcaggt gtttgatttg ttggaacggg
      481 taagccgaaa taacattacg gtactgatac gcggcgaaag cggcaccgga aaagagttag
      541 tcgcccgcac gatacatgaa aaaagcaatc gccgtagcgg cccgtttatt tcgatcaatt
      601 gcgccgcact gcctaaagaa cttattgaat cggagctttt cggttacgaa aaaggtgcat
      661 ttaccggagc catatcttcc aaacaagggc gctttgaatt ggccgatggc ggcacattat
      721 ttttggatga aatcggcgat atgtcgcttg aaacgcaggc caaagttttg cgtgtcatcc
      781 aggagcgtaa gtttgaacgc gtcggcggta ccgaatccat cagcgtggat gtacgcattc
      841 taagcgcaac gcaccgtgac ctggcgcgat ttatccgcga cggtcagttt cgcgaagatt
      901 tgtattatcg tatcaatgtt gttgagatcg aattgccccc gctgcggtcc cgctcggagg
      961 atattcatct tttggtgaat cactaccttg tgatgtttgc gcaaaaacat aaagcgcagg
     1021 tgacgcgttt ttcgcaagac gcgctcaaag cgatgctcga atacgattgg cccggcaatg
     1081 tacgcgaatt gatcaacgtg gtagagcgtt gtgtcgtgct cagtaccgaa gaagaaatcc
     1141 ggcgcgatat tttaccttcg gagattatcg gtggaggacg accgacgaca cttggcgtac
     1201 gcgatatgct ggaaaaaggc gatatcaatt ttcaggatgc caagcatcgc gtcgtgcaga
     1261 cgtttgagcg cgaatttttt atggaagcgg ttcgcatgaa taactggaac atttcgcaaa
     1321 cggcgaacaa actcggcatg aaacggcaat atcttcagca aaagctcaaa gaactggaac
     1381 tgaacatcca ggaagcgaaa gaaaatatat gatgcgttca caaacaccgg aaccttcggt
     1441 tttaccggcc atccaatcgg cgccaccggc gtcggaacaa ccgattggaa ttttcgattc
     1501 gggcatcggc ggacttactg tcgtcaaaga ggtaatgaac caactgccgt atgaacgcat
     1561 tatttatctc ggtgatacgg cgcgcgtacc ctacggaacc aaatccgatc gtacgatcaa
     1621 atcgtttaca ttgcaaagct gccttttttt gcttgagcat aatgtcaaaa tgatcatcat
     1681 cgcatgcaat acggccagcg ccgtggcgct cgacttttta tccaatatgt tcaaaatacc
     1741 cgtcatcggc gtgatcgttc cggggtcgga agcggccgtg cgaaaaagtc tgcggaaaaa
     1801 aatcggtgtt attggaacgc atactacggt caattcgcaa gcctataaaa aaaccattca
     1861 cgcgttggat gcccaatgtg aggtgttttc tcagccgtgt tcgttatttg taccgctggc
     1921 cgaagaaggt tggcttcgtc atgatgtgac acaacgcatc gccgcagagt atttgaatgt
     1981 tttttccgat aaagatattg atacgctggt catgggttgt acgcattacc cgatattgag
     2041 cggtgcgata caagaggcga tgaatacgat catgcgccgt ccggtcactt tgattgacag
     2101 cggtgtcgaa acagccaaaa gaacacgaca gatattagat gaaaaaaaac tgcttaacac
     2161 atccgggcaa aagccctacc accgctttta tgtgacggat cttcctaaga actttatgcg
     2221 tgtgggcgaa atgtttctag gatcgaaaat agacagcgtc gaaaccgtca atgttgaagt
     2281 gatgggtttt tgatatagcg ccgccgtttt ccaacgcatc gtaaaatttc acgtagcgaa
     2341 gttttttact acgatttctc aacgtcattt cccgccttta cgcatcgaat acaaaacaat
     2401 gatactgacg atcagaaaag gaagtgcagc gaaggttatt tttagattga gagaaatcaa
     2461 accaatcaaa aatataaacc acagcaaaac gcctaacccc agtaaaccgg tcaacactat
     2521 gacaggacgt ggtttattct gaaacagggt aacaagcaac aatgcttgcc ctaataatgg
     2581 aagcgcaatc atcggatgag tgatcgcgtc ggtatgcgtg aaaaacttac ggatgatttc
     2641 aaactctgct tgcacgagga aagtgtagtg accgctaccc cattcgagat aaccgatcag
     2701 tgaagagagt accagccccg cgtacagtat tttaggatgc agcgacgtta gaaaatttgt
     2761 tttcacagaa ttgtttcaaa ttttgtgatt cccttaccag atacgaatca accaaagcgc
     2821 aggttttttt gttaatcaga ttagcaaaaa gaccggagaa tttgataacc aatgtgacaa
     2881 tacatccgtc ggatgttaac tctaaagcgt gaatcgcgtc gatacgcaga agcaaatttg
     2941 ttttgctcca cgtaaattgt ttcacgggtt caaattcggt aatcttccaa accgctatgg
     3001 caagtttagg ttgctcgatg aggacccggc tacctgtttg taaagaaccg ggcgatagtt
     3061 tttttatgga taggatcgtg tcggtccaga ggggccactc gtctacagcc atccatacgc
     3121 gccagatatt ttccggcgac gtatgaatga ctaaggnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnntcta cagccatcca tacgcgccag atattttccg gcgacgtatg
     3301 aatgactaag gtttgtataa actctttctt atttggcata caggtctata attcgctcga
     3361 tcgcttcgtg gcatacttta caaaagggga cgttattgcg tgtgaacatg atgcaatcta
     3421 attccgggcg atataaacct ttggcttcgt aacctgcgcc ttcaaaagcg cccacttttc
     3481 cgaaatattt gtccgtatta agcatcggcg tcgttttggt ttttacttct ctaaagagcg
     3541 cttccatcgt ttcttcggac gctttttcct cgattagttt agcgcgtttt ttttgcatct
     3601 cgatttcata cgtatcgtac gcggttttat tccacggtgt cggcaaaggg gtgtctttcg
     3661 atacaagatg tttccactta agatttttcg gatcaagcaa agcggtaata ttcggctcgg
     3721 aaggttcggt actcggcgcg gtaaagtttt catacgaaac gggtgaggta taatattcat
     3781 cgccgagacc cgcaaaagaa tgaccgaatt cgtgtacaaa aacatacgaa gccggttccg
     3841 catcggccgt gaccgtcgcc cacagattaa aaataccgcc gccgccgtat ttgcgatcat
     3901 tggccaaaag gatcaatgca tcgtagggcg cattggcggc cgtttcacga agacttttat
     3961 tttcatacgt cagtacataa cgatcggagt caaacgaatt gaagctcaaa ccgagcgccg
     4021 atttgcgcca aatatctttg cgcgggttac tgataccgga ttgggcggaa gctacatcca
     4081 tcgcacgaac attaaaatct tttttgcggg atttgaaggg ttcggtttca aacatcactt
     4141 tgactaaacg ttcgacgtcg gcgtgaaatt tattcttttc gccggccgtg taaccgtcgc
     4201 tgagaatcag cagatcgacc ttttgcgaag ggtcgccgtt ttcaaaaacg ctccacactt
     4261 cgccgttctt aacggcaggc gcacgattga caaagcggct tttgggatcg atggttgttt
     4321 cgtaaaacga cgcaaaacga ttatcagcgc cacgtttttt gagtgttaat ttgaccgcat
     4381 ttttgggttc gggaaaacgc tgcgattcgt gaaatgagcg ccacacgcct ttggccgctt
     4441 cgcccgttgt ttcccattcg ccgtagatgc tcgcaaatcc gcgcgtataa agcaattttc
     4501 cggatgcaac atcactcact tcaaacaaat atttgccgag accggtttca tccaataaca
     4561 ccgttttact gcccggccac acgccttcga tgcggtattc gtcgggactg atatgttctt
     4621 ctttggccgt accgctgtga taatagtcaa accgcatcgt ctggccggta aaataagcat
     4681 caaaatcatt agtgcccatg acggcaagta ctcccaatat aaatagcatg atattctcct
     4741 gagataatta gcgtgataaa cgcgatgaat aatgcacacc aatatatcaa aaacatgccg
     4801 tcatattcta taattttcca ttaccagatg cgtacgcgcg attccggtgt taggtacagt
     4861 ttatcaccgg gtttgacatc aaatgcgtca taccacgcgt cgagattgcg tacggtagcg
     4921 atgcggtact gttccggtgc gtgatgatcc gtcgcgattt gtacgcgcag gcctttttcg
     4981 gtggttttgc tgcgccagct ttgtgcaaaa ccgataaaaa actcacgatc ctgttggcgt
     5041 acgtagtcct gattttgaat tttatcccct aaggtttttc gataggcatc aaaagcggcg
     5101 gcaagtcccg caaggtcggc gatattttcc gatcgggtaa gctcaccgtt aacggcgagg
     5161 tcgtcaaacg ggcgataacc ggaaaactgc tctaccagcg gtttggcggc cgattgaaac
     5221 tttttcatat cgttgggtgt ccaccaatgc tttagctctc tttcagcatt ccattcggct
     5281 cccaaaggat caataaagtg gcttgattcg tgaccgacaa tagcgccgat agcgccatag
     5341 ttggccgcat ccgataatgt ggaatcaaac ttgggtgctt gtagtaaggc ggcggcaaaa
     5401 ttgcaggcgt tgtattggaa taaaagcaga gcgcccgcgg tatggggtgt cgtcgcccat
     5461 tcggtggtat ccggcggttt tccaagccgt gccaaagcgc gacgatagtt gcgatcggcg
     5521 atgttttgta aattttttac gggattggca gggtctatat tcagatccga ataatcttgc
     5581 catacattgg gataaccgag accaaagtac aatgtctgaa gttttaccat cgcttgtgtt
     5641 ttgctctcgt ccgacatcca cgacacggtt ttgacccgtt cgcggagcga agcgatgacg
     5701 ttcgtcgcga tcgcttgtac gcgggatttt tgggaagcgg gaaaataacg ttctacatat
     5761 aaccagccga cggcttcacc caaaaatttc tgtgtcgcat cgagcgcacg ttgtgaacgg
     5821 gcaggggaag gtgcgtcttg tatcgacgag cgcaaagtca gcgccgcttc cgcgaaagcc
     5881 gacggtaata catcggcgta cgcatggatc gcttgaaaac tcagataatc cttccacact
     5941 tccacgggct gtgcggcgat aagcgaagcc aatccggtga tcgcgtccgg ctgccaaact
     6001 acgaacgatt cctgatgctt cagaccggcc tcttcaaaaa aaacggacca atccataccc
     6061 ggagcccgtc gttcaaagtc gttgcgtgac cagcgatgcg aaccattgcg gtcattgttg
     6121 gaaaattcat cgctcgcatg ggtttttgct aataagattt cgatgccaag taccgactgt
     6181 gcacgccgtt cgggatggct aaatccggct tgtttgaatt gctgagatat atagttctga
     6241 taggttgctc tgattttttt attggccgcg tcctcgctga ggtaatatga acgttcttcc
     6301 agcccaaggc cgccctgcat taaaaaaggc acgtaggttt tttcgccgaa atatcctact
     6361 tccacagaca aacccaataa tcccggggaa cgatacgtaa gccaatcggc atcggcacgg
     6421 agtctgcgac cgagtaattt gaccaacgct gttttatcgg ttatgggatc aatttcatcc
     6481 agta
//
LOCUS       DAOQGF010000158         6400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_5988_length_6400_cov_6.795429, whole genome shotgun sequence.
ACCESSION   DAOQGF010000158 DAOQGF010000000
VERSION     DAOQGF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5988_length_6400_cov_6.795429"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PK937_14585"
     CDS             <1..690
                     /locus_tag="PK937_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33850.1"
                     /translation="RYYYWSLVNNNTLYKSYSAEKLERKVTNREGFLDDKRTGFDPAH
                     PIFGILVEDLASQNKALSQFLKAVKDVKLQLFILANDSISSVSSLKPLITKNPNHTFF
                     TMQDPEEQFRHNFFAVADMLYIPPSDDFAEIYFMNGIRYGGIPVIREECIISDRFTPT
                     KEKSTSGNAIIYSDEKDMVKKIAAAVSYYETDKNGWDHLVRHAMKSDANWALSASSMI
                     KLYEKALAKLK"
     gene            741..2462
                     /locus_tag="PK937_14590"
     CDS             741..2462
                     /locus_tag="PK937_14590"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33851.1"
                     /translation="MTAQTPRFQYRLDIPRPSSDQGYVTLTLTGIKLKSATFCMPAWS
                     PGNYRIVDYGRWVDSVQAWDIKGKEISVSRLNNNEWFVKNARQLARITYRIHDIPEDS
                     VPVLPTTLNSMDRSTFFYNGPSVFGYIKGYQTEACQVEFQLPCGWRVWTPLSRISDFR
                     YAARSYDELVDAPALLGTWPIEAFRFTLDSTDYHFVINSETDIKTDTLLNLAKTIIRY
                     QNRLFDDRPSEAYYFLYNFFAAGSRYGALESTRASSYYLPPFASQRNLRSSYLIRVLA
                     HEFFHRWNPERFHPKELHIINYQAPLRIQNLWFIEGVTEYYAQLTLVRTGLLSKKVFY
                     NTFRDIAAENPSANLEALSRNASEIGVAPAMYTQGSLTAWLMDIEMRDRTDNAVSMDS
                     LMVRMHRAFGKKDKPYDDKTLITTIKQFTGVDFTSFYSHYIAGGKPLPVREILEKAGL
                     AYDIIYTPAHGWFWDVSAGGKLVVQSVSENSTAFSLGMQSGDIVTSIQEQTLPTTMDG
                     IRTRIEALESLSVGESVRLMVQRNGKPIALKGTIVASQKPTVSIKEIAAPTEKQLRIR
                     QSILSSP"
     gene            2484..2729
                     /gene="purS"
                     /locus_tag="PK937_14595"
     CDS             2484..2729
                     /gene="purS"
                     /locus_tag="PK937_14595"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNH33852.1"
                     /translation="MLCTIKIKVTPKAGVLDPQGKVVMSSLETLGFNGIDDVRLGRYI
                     EIKMEASSKEEAARQADTMCQKLLANLVIEQYQVEVS"
     gene            2810..2884
                     /locus_tag="PK937_14600"
     tRNA            2810..2884
                     /locus_tag="PK937_14600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2845..2847,aa:Pro,seq:cgg)
     gene            2992..3672
                     /locus_tag="PK937_14605"
     CDS             2992..3672
                     /locus_tag="PK937_14605"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNH33853.1"
                     /translation="MNTFLRIVWTIFEKDFLTEKRTKESFSAMLVFGILVLVVFNFTV
                     SPGSQEIQEIGAGMLWVAFTFAGTLGLNRAFASENDNGNMQALIMVPVDKGAIYLGKL
                     LSGAMFMFTVELFIIPVFVLFFNVNIWEQLPGLLLISILGTLGFIGTGTVFSAVALNT
                     RMREVLLPVLLFPVTIPMIIAAVEATAVLLRGEPLSGAFDWIKILLGFDVTIIVTSFL
                     TFEYILEE"
     gene            3916..5874
                     /locus_tag="PK937_14610"
     CDS             3916..5874
                     /locus_tag="PK937_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014243884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH33854.1"
                     /translation="MSRLIDNQASIFIRQSKGWSYLAMCGGHEGIQLALGLSFRANKD
                     FLFPYYRDQLTCLAAGISPYEIMLNGLSRKDDVASGGRHMSNHFAKPEIQIENVSSCV
                     SNHTLHAVGVARAIKYYKSDAIAFASFGDSSSSEGYVYEAMNGASREQLPVIFIIQNN
                     HYGISVPTEEQTANYVVSENFRGLKNLKIFNCDGKDFFDSRRAMDEAIAYVRSGEGPA
                     IVHADCVRMNSHSNSDRHELYRSPEELADMQKLDPLHKFRSFLVEERIFSEDEITAIE
                     NDNKNIVDEAAAKAEAAPTPDPATAMDFVTPEPIQVEETPTPDGEPTEKILQAMNRVF
                     HEEFRHNPDTFLWGQDVASKDKGGVFNATKGMQQEFGRGRVFNAPIAEDFIVGTANGF
                     SRWHDNVRVVIEAAQFADYVWPAMEQIVETSHEYYRSNGQNCPNIVIRLASGGYIGGG
                     LYHSQTVEGIMANLPGVRVVVPSFADDAAGLLRTAIRSRGITFFLEPKFLYNQIFAQS
                     PKTDEHHFVPFGKARVRREGQDVTIVTYGTPVHWSLRAAGKMADKGIHAEVIDIRSIV
                     PLDMETIKRSVQKTNRVLVVTENQATGGFSGEIAARITQECFEYLDAPVTRVCSADCP
                     IPFSRILEAAVLVDEAKIAHAIENIMAY"
     gene            complement(5947..>6400)
                     /locus_tag="PK937_14615"
     CDS             complement(5947..>6400)
                     /locus_tag="PK937_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33855.1"
                     /translation="IGAYEKLTAEGYKCRVVSLPCWSLFERQGKAYWEKVLPSDVRCR
                     VAVEAAASFGWRRYTGIEADGGIIAMRSFGESAPIKALLEEFGFTTEAVVKLAKQKLK
                     QFGKAAPKKTVSAKNKPAKKAKSKPKSKSTNKKTVSKKKQTSGKKKRK"
BASE COUNT         1628 a         1575 c         1464 g         1733 t
ORIGIN      
        1 aggtattatt attggagtct ggtcaataat aataccttat acaaatccta ttcagcggaa
       61 aaacttgagc gaaaagtcac caaccgcgag ggttttttag atgataaacg tacaggattt
      121 gatccggcac acccgatttt tggtattctg gtagaggatt tagcttcgca gaataaagca
      181 ctgtctcaat ttctcaaagc tgtcaaagat gtgaagttgc aactgttcat tttggccaac
      241 gattcgattt cgtccgtctc atctttaaaa ccgttgatta ccaaaaatcc aaaccacact
      301 ttttttacga tgcaggatcc cgaagagcag ttccggcaca atttttttgc cgtagccgat
      361 atgttgtata tacctccttc ggatgacttc gccgaaatct attttatgaa cggcattcgt
      421 tacggcggca tacccgtaat tcgcgaagaa tgtatcatca gtgatcgttt taccccgacc
      481 aaagaaaaaa gcacatccgg taacgcgatt atttactccg acgaaaagga tatggtcaaa
      541 aaaatagccg ccgccgtttc ctattacgaa acggacaaaa acggctggga tcatcttgtt
      601 cgtcatgcga tgaaatccga tgcgaactgg gcactcagcg cgagttctat gatcaagctg
      661 tacgaaaaag cgctggctaa attgaagtaa ggggcggcgt tctgatacga attattttta
      721 tattcattag cgccggacta ttgacggctc aaacgccccg ctttcagtat cggcttgata
      781 ttccccgtcc gtcctccgat caaggttacg tcacgctcac gctcaccgga atcaaactca
      841 aatcggcaac gttttgtatg ccggcatggt cacccggaaa ctatcgtatc gtggactacg
      901 ggcgttgggt tgacagtgtg caagcttggg acataaaagg aaaagaaata tctgtttcac
      961 gcctcaataa taacgaatgg tttgtcaaaa acgcacgcca actcgcacgc atcacctacc
     1021 gcattcatga catccctgaa gacagtgtgc ctgtattacc gaccacattg aattctatgg
     1081 atcgttcgac gtttttttat aatggtccga gcgtattcgg ctacatcaag ggttatcaaa
     1141 cggaagcttg tcaagtcgag tttcagcttc cttgcggttg gcgcgtatgg acgccactgt
     1201 cacgcatttc cgacttccgt tatgcggcgc gttcttacga tgaactggtg gatgcgccgg
     1261 cgcttttggg tacatggcct atcgaagctt tccgttttac actggatagc acggattatc
     1321 attttgtcat caatagcgaa acggacataa aaaccgacac gcttttgaat cttgccaaaa
     1381 ccattatacg ttaccaaaat cgcttatttg acgatcgtcc gagtgaagcg tattattttt
     1441 tatataattt ttttgcagcg ggcagccggt acggtgcttt ggaaagcacg cgtgcgtcat
     1501 cgtattactt accgcctttc gcgagtcagc gcaatttgcg ttcatcgtac ctgattcgcg
     1561 tgctcgctca tgaatttttc catcgttgga atccggaacg ttttcatccc aaagaactac
     1621 atatcatcaa ctatcaagca ccgctacgaa tacagaacct ttggtttatc gaaggcgtta
     1681 ctgaatatta tgcacagctt acgctggtgc gtacagggct tttatcgaaa aaagtttttt
     1741 ataatacgtt tcgcgatatt gccgctgaaa atccttcggc caatctagag gcgctcagcc
     1801 ggaacgcatc ggagatcggc gttgccccgg ctatgtacac acagggttcg ctgacggcat
     1861 ggcttatgga tattgagatg cgcgatcgaa cggataacgc ggtttcaatg gatagtctga
     1921 tggtgcgcat gcatcgtgcc ttcggaaaaa aagacaaacc ctatgatgac aaaacgctca
     1981 tcacgaccat caaacagttc accggtgttg attttacttc gttttattcg cactacattg
     2041 ccggcggaaa acctttgccg gtgcgggaga ttttggaaaa agcggggttg gcctatgata
     2101 tcatctatac accggcccac ggttggtttt gggatgtaag tgccggtgga aaattagttg
     2161 tccaatcggt gtctgaaaat tcaaccgctt tttcactagg catgcaatcc ggagatattg
     2221 tcacgtctat tcaggaacaa acgttaccta cgacgatgga cggtattcgg actcgcatcg
     2281 aggcgctcga atcgctcagc gtgggcgaat cggtacgcct catggttcaa cgcaacggaa
     2341 aacctatagc actcaaagga accatcgtcg cttcccaaaa accgacggtg agcattaaag
     2401 aaatcgccgc tccgacggaa aaacaattac gcatccgcca aagcatttta tcatcaccat
     2461 aaaaaataaa caggaaatcc aacatgctgt gtacgatcaa aataaaagtt acgcctaaag
     2521 ccggagtcct ggatcctcag ggcaaagttg tcatgtcttc gcttgaaact ctcggtttta
     2581 acggaataga tgacgttcgg ctcggccggt atatcgaaat aaaaatggaa gcctcttcaa
     2641 aagaagaagc ggcacgtcag gccgatacga tgtgtcagaa acttttggcc aatcttgtca
     2701 tcgaacagta tcaggtcgaa gtgtcatagc agctaaaaac ttgttgcatt taagtttttt
     2761 ttttagcatg attgccccgc cttgttgtat cagggcattg ttttatgttc ggggtgtagc
     2821 gcagcccggt tagcgtgctt ggttcgggac caagaggtcg ggagttcgaa tctccccacc
     2881 ccgacgatac atatcgcaac acatggattt tatgtcgaaa taaaacaaat tttttaacac
     2941 cagcgcaagc tggtgttgtt gttttaggct acgatactaa cttaagaaca catgaacacc
     3001 ttcctccgca tcgtttggac catcttcgag aaggacttcc ttaccgaaaa gcgcactaaa
     3061 gaatcattca gtgccatgct ggtgtttggt attctcgtgt tggtggtatt caattttact
     3121 gtcagcccgg gttctcagga aatacaggag attggcgccg gcatgctgtg ggtcgcattt
     3181 acatttgccg gcactttggg acttaaccgc gctttcgcgt cggaaaacga taacggcaat
     3241 atgcaggcac ttatcatggt gcccgttgat aaaggcgcca tttatctggg taaactgctc
     3301 tccggcgcga tgtttatgtt taccgttgaa ctgtttatca taccggtgtt tgtactcttc
     3361 tttaatgtca atatatggga acaactcccg ggattactcc tgatcagcat actcggcacg
     3421 cttggcttta tcggtacggg tacggttttt tccgccgtag cactgaatac ccgcatgcgt
     3481 gaggtgctcc tgccggtgct tttgttcccg gttaccatac ccatgattat tgcagccgtc
     3541 gaagccacag ccgttttact ccgcggggaa cctttgtccg gagccttcga ttggattaag
     3601 attctcctcg gttttgatgt caccattatc gtcacttcgt ttctcacctt cgaatacatt
     3661 cttgaagaat agttcacgct gacttttgtt gcctcaccta accattttgg ttaaatcgtt
     3721 gacccaacaa tttgcttgaa tcgctttgat tttttcacta tagtgccgcg tttattgaaa
     3781 aaaacagagt tgttaaaaca taggtttcac caaaaacgga gagacatttt ggaagctgtt
     3841 gcatcatcca aatcgggagc caaacgtccc gaaatcaaac gcgacactct taaacaatgg
     3901 tacactctgg cgcatatgag tcgcctgatc gataatcagg cttccatatt tatccgccaa
     3961 tccaaaggtt ggtcttacct cgcgatgtgc ggcggacacg aaggcattca gctcgccctc
     4021 ggcctttcct ttcgcgccaa caaagatttt ctttttccct actaccgcga tcaattgaca
     4081 tgccttgccg ccggcatttc cccttatgaa atcatgctca acggtctcag ccgcaaagat
     4141 gatgtggcca gcggcggacg ccatatgtcc aaccactttg cgaaaccgga aattcagatc
     4201 gaaaacgttt catcctgcgt ttccaatcac acgctgcatg cggtcggtgt cgcacgcgcg
     4261 atcaagtatt ataaatccga cgcgatcgct tttgccagct tcggcgactc ttcgtcctcg
     4321 gaaggctacg tgtatgaagc catgaacggc gcttcgcgtg aacagttacc ggtgatattt
     4381 attattcaaa acaatcacta cggcatttcg gttcccaccg aagaacaaac agcaaactac
     4441 gtcgtatcgg aaaatttcag aggcctcaaa aacttgaaaa ttttcaattg cgacggcaaa
     4501 gatttttttg acagccgtcg cgccatggac gaagccatcg catacgtgcg ttccggtgaa
     4561 ggtccggcca tcgtgcatgc cgattgtgtt cgtatgaatt cgcactccaa tagcgatcgt
     4621 catgaattat accgttcgcc ggaagaatta gccgacatgc aaaagttaga tcctttgcat
     4681 aaattccgtt cctttttagt cgaagagcgc atttttagcg aagacgaaat tacggcgatc
     4741 gaaaatgata ataaaaacat cgtcgatgaa gcggccgcca aagcggaagc cgcgcctact
     4801 cccgatcccg cgaccgccat ggattttgtg acacccgaac cgattcaggt cgaagaaacg
     4861 cctacaccgg acggcgaacc taccgaaaaa atactacagg cgatgaaccg cgtatttcac
     4921 gaagaatttc gacataatcc cgatacgttt ttgtggggac aagatgttgc ttccaaagat
     4981 aaaggcggtg tatttaatgc gaccaaaggc atgcaacagg aattcggacg cgggcgcgta
     5041 tttaatgcgc cgatcgccga agattttatc gtcggcacgg ccaacggatt ttcgcgttgg
     5101 catgataatg tgcgcgtcgt gatcgaagcc gctcagtttg ccgactatgt gtggcctgcg
     5161 atggagcaaa tcgtcgaaac gagccatgaa tactatcgca gcaatggtca aaactgcccc
     5221 aatatcgtaa ttcgcctcgc cagcggcgga tatatcggcg gtggcctcta ccactcacaa
     5281 actgtggaag gcataatggc taatcttccg ggcgtgcgtg tcgtcgtacc gtcttttgcc
     5341 gatgatgcgg cagggctttt gcgtaccgcc attcgctccc gcggtattac ttttttcctc
     5401 gagcctaaat ttttgtacaa tcagattttt gcgcaaagcc ccaaaacgga tgaacatcat
     5461 tttgtaccgt ttggcaaagc ccgcgtgcgt cgtgaaggcc aggatgtaac catcgttacc
     5521 tacggaacac ccgtacattg gtctttgcgt gctgccggaa aaatggccga taaaggtatc
     5581 catgccgaag tgatcgacat ccgctccatc gtaccgctgg atatggaaac gatcaaacgc
     5641 tcggttcaaa agaccaatcg cgttttagtc gtaaccgaaa atcaggctac cggcggattt
     5701 agcggtgaaa tcgcagcgcg cataactcag gaatgttttg aatatcttga cgctccggtg
     5761 acgcgcgtat gcagtgcgga ttgcccgatc cccttctcac gtattctgga agccgcggtg
     5821 ctggtggatg aagccaaaat cgcccatgct atcgaaaaca tcatggccta ttgagaattt
     5881 ttcgcagaca taaaaaaagg cttcggttaa acaccgaagc cttttttgtt atacgaatgt
     5941 aatcgtttat ttgcgtttct tttttccgct tgtttgcttc ttctttgata ccgttttttt
     6001 gttcgtgctt ttagacttcg gcttgctctt ggctttttta gcaggtttgt ttttagcgct
     6061 tactgttttt ttaggcgcgg ctttcccaaa ttgtttcagt ttctgtttgg ctagtttcac
     6121 caccgcttcc gtagtaaaac cgaactcttc taacaacgct ttgatcggag cggattcgcc
     6181 aaaactgcgc atggcgatga taccgccatc ggcctcgata cccgtataac gccgccatcc
     6241 gaagctggct gcggcctcaa cggctacacg gcaacgcaca tcgctcggca gtactttttc
     6301 ccagtacgcc ttgccttgac gttcaaataa actccagcac ggcaggctca ccacgcggca
     6361 tttataaccc tccgccgtca gcttttcata ggccccgata
//
LOCUS       DAOQGF010000159         6376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6026_length_6376_cov_7.163740, whole genome shotgun sequence.
ACCESSION   DAOQGF010000159 DAOQGF010000000
VERSION     DAOQGF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6026_length_6376_cov_7.163740"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2593
                     /locus_tag="PK937_14620"
     CDS             <1..2593
                     /locus_tag="PK937_14620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33856.1"
                     /translation="AWMTRSGWLLYPTGKGLAAVNPRLLSRVSPSPLVVIERMLAEQK
                     LIGLGEKIELTPGHRRIEFTFTGLSFTAPENNTFMYRLIGFDKEWIQAEKTRHAVYTN
                     VPPGAYRFEAKASNSDGIWSSQPATVLFSIDPYFFETVWFYSLSGVFLLYLGFTYYRS
                     RVRELHERQEELTRLVQERTRNLREEKEKTEHALRESEEAKREIEKSQQVIQSQSQKL
                     IELDKVKTNFFTNISHEFRTPLTLIIGPLEDVLRSATLSADQRVSFELMLRQAKQLLG
                     LINQLLEAARLESNRVQFHPSLQPLVPFVRELTSMFESAAQRKGIHLSLETYDEKIQL
                     YYDLHAMERVLTNLISNAIKFTEQGFIKITIKHSEHTHRPSVVIFVEDSGRGIPQEHL
                     DHIFQRFYQADGVEADQLGSGIGLSLVHDYVEMHRGLITVESTVGKGTRFMITLFTGE
                     EHLSGTITPHAEIKTETEFIYARSIAALSSEHNIAPEISAPDPMERQTADKPKVLIAD
                     DNADIRLYLYNLLRDEYRMIMAHDGAAALDLLQREFPDMVLSDVMMPNMDGMELCRRI
                     KSDERTRHIPVILLTARADESSKVESLNLGADDFVAKPFSANELKARMRNLLGIHAMK
                     LQLIEQEKMASLGHLARGVAHEINNPLSFVTGNLSVLESRLAKLHPDENIKPIVTDIM
                     SGTKRIAAIVQHLREFTRLDEAERKYVDLSDMVDNILTLLAGEMQSDIRIEKNIAQVP
                     MIDCFPRDLNQAIAQILMNATYYVRERNRIENRSLGHITIHLNKADEKYVELIIENDG
                     FPIAPEIREHIFEPFFTTKPIGKGTGLGLSAVYGIIRRHHGQIRCVSEPFQPVRFIIM
                     LPFAIPS"
     gene            2791..4059
                     /locus_tag="PK937_14625"
     CDS             2791..4059
                     /locus_tag="PK937_14625"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNH33857.1"
                     /translation="MKSILHSITRILTLFYILAFINQAWATDGYFSHGYGTKQKSMGG
                     VSTALTFNAFSAITNPANAVYIQNNWGLGIAFFNPNRQYAVSGSPSGFPNTFPLTPGT
                     VKSKSTGFFIPSLSYNRLINEKSAFNFAIFGNGGMNTDYKTNTFNTTKPTGVNLSQLF
                     VNTSYAYKVTDNHAFGVTAIFGYQWFEAKGLTAFSGFSRYNTKLTDNKSDKAYGYGAR
                     LGYSGQITPQLRIGASYQTRIWMTEFNDYKGLFAEKGDFDVPATWNAGLAWDINSAWT
                     LAADLTQIYYSSVKSVGNPMKPADFQNDILLGDKKGAGFGWNDMTVYKIGCEYRHGND
                     WSARVGYNYGKQPIPTSEVMFNILAPGVIEHHLTFGASKKIGSKYEFSLAVMRGFSNE
                     VKGANPMEAPGQQAIALKMNQWEFEIGLGF"
     gene            4081..6273
                     /locus_tag="PK937_14630"
     CDS             4081..6273
                     /locus_tag="PK937_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNH33858.1"
                     /translation="MLSRRRFLKIGAGFTAGATIAAGAAHTLADRLAALEKPSGQVEV
                     IPTYCDICFWKCGVLAHVKDGQLWKLEGNPEDPLSRGRLCPRGTGGVGAHYDPDRLRT
                     PLIRRGEKGAEEWTSVTWDEALDFIAEKMKKIKAEYGPESVALFSHGIGGNFFKHTIK
                     AYGSPNIAAPSFAQCRGPRDVGFRLTFGEDVSSPERTDIRNAKCLVLIGSHLGENMHN
                     TQVQEFAEAVDHHATIIVVDPRYSVAASKAQHYLPIKPGTDLALILAWMHVIVKENLY
                     DKAYVEQYGFGFDAFVAALETYTPEWAYPETGIDPETIRTTARVMAQHKPATLVHPGR
                     HVTWYGNDAQRSRGIALLNALLGSWGSKGGFYTPVSMDVPAFPYPSYPKSDKGKVDNP
                     GHKYPFAHETITTGIREATLTGKPYPIKGWFVYATNLLHALPNEEETIKAIQNLDLMV
                     VIDVIPSEIAGWADVILPESVYLERHDDLNVEWFRDPFVALRQPVVSAPHDQKPNWWM
                     AKRLAEKLGLGHYYPWTHIESYFETRLRQAGLSYDTLKKNGILLGEKKPVYFDEGVPA
                     EFPTPSGKIEFYSSQLAEAGFEPVPKYVAPPQPPPGSFRLLFGRAPVHSFSRTHTNPV
                     LMDMMNENELWVNADMALRYGLKSGDYVRLKNQDGVVSTKIKIKATERIRTDCVYMVH
                     GFGHNAKALKRAFGRGASDAQLITRYDTDPLMGGTGMNNNFVTLEMEA"
     gene            6276..>6376
                     /locus_tag="PK937_14635"
     CDS             6276..>6376
                     /locus_tag="PK937_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNH33859.1"
                     /translation="MARLAMVIDTRKCVGCMDCVVACKTENQVPEGY"
BASE COUNT         1683 a         1604 c         1497 g         1592 t
ORIGIN      
        1 cgcatggatg acccgcagcg gatggcttct gtatccgacc ggtaaaggtc ttgctgccgt
       61 gaatccgcgt ttgttgtcgc gcgtttcccc gtcaccgctg gtcgtcattg agcgcatgct
      121 ggctgaacaa aaactcatcg gcttaggtga aaaaatcgaa ttgacgccgg gacaccgccg
      181 tattgaattt acatttaccg gtttaagttt tactgctccg gaaaacaaca catttatgta
      241 ccggcttatc ggttttgata aagaatggat ccaagccgaa aaaactcgtc atgccgtcta
      301 taccaatgta ccgcccggtg cataccgctt tgaggcgaag gcttccaata gcgacggcat
      361 ttggtcctcg caaccggcga cggtattatt ttcaattgat ccgtattttt ttgagaccgt
      421 atggttttac agtttatccg gtgtattcct tttgtatctc ggttttacgt attaccgttc
      481 gcgtgtgcgc gaactgcacg aacgtcagga agaactgacg cggcttgtac aggaacgcac
      541 acgtaatctg cgcgaggaaa aagaaaaaac cgaacacgcg ttgcgggaat ccgaggaagc
      601 taaacgcgag atcgaaaaat ctcagcaggt cattcagtcg caatcacaaa aattgatcga
      661 attggataaa gttaaaacca atttctttac caatatttct cacgaatttc gtaccccgct
      721 cacattgatc atcggacctt tggaagatgt attgcggtcc gccacgcttt cggccgatca
      781 acgcgtttca tttgaactca tgttacgtca agccaagcaa ctattgggtt tgatcaatca
      841 acttctggag gcagcccggc ttgaatccaa tcgggttcag tttcatcctt cactacagcc
      901 gctggttcct tttgttcgcg aactgacttc tatgtttgaa tcggcggctc agcgtaaagg
      961 cattcatctt tcgctggaga cctacgacga gaaaatacaa ttgtactatg accttcatgc
     1021 gatggaacgc gttttgacaa atcttatttc taatgcgatc aagtttaccg agcaagggtt
     1081 tataaaaatt acgatcaaac atagtgaaca tacgcatcgc ccgtcggttg tcatttttgt
     1141 cgaagacagc ggacgcggca ttccgcagga acatctcgat catatctttc agcgttttta
     1201 tcaagcggat ggtgtcgaag ccgatcaact ggggagcgga ataggtcttt cgctggtcca
     1261 cgattatgtg gagatgcacc gcggcttgat cactgtcgaa agcacggtcg gcaaaggcac
     1321 acgttttatg ataacgcttt ttaccggcga agaacattta tccggtacga tcacacctca
     1381 tgctgaaata aaaacggaaa ccgaatttat ttacgcccgc tccatcgctg cactttcatc
     1441 cgaacataac attgcaccgg aaatatcagc gccggatccg atggaacgtc aaaccgccga
     1501 taagcctaag gtattgatcg ccgatgataa cgccgatatt cgtttgtact tatataatct
     1561 tttgcgggac gaataccgca tgatcatggc gcacgacggc gccgcggcgt tggatttgct
     1621 tcaacgggaa tttccggata tggtgctcag cgatgtgatg atgcccaata tggacggaat
     1681 ggaattgtgc cgtcgtatca aatccgatga acgcacccgc cacattccgg tcatactact
     1741 caccgcacgg gccgacgaat ccagcaaagt ggaaagcctc aatctgggtg cggacgattt
     1801 tgtcgccaaa ccttttagcg ctaatgaatt gaaagcacgc atgcgcaatt tactcggcat
     1861 ccatgccatg aaactgcagt tgatcgaaca agaaaaaatg gcttctttag gacatcttgc
     1921 ccgcggtgtc gcacatgaaa tcaataatcc gctctccttt gtaacaggta atctttccgt
     1981 tttggaatca cggctagcca aactacatcc ggatgagaat attaaaccca ttgtcaccga
     2041 tatcatgagc ggaaccaaac gcattgccgc gatcgtgcaa cacttgcgtg aattcacacg
     2101 tctggatgaa gccgaacgta aatacgttga tctgtccgat atggtggata acattctcac
     2161 gctcttagcc ggcgaaatgc aatccgatat tcgcatcgaa aaaaatatcg cacaggtgcc
     2221 catgattgat tgttttccgc gcgatctcaa tcaagcgata gcacaaatcc ttatgaacgc
     2281 gacatattat gtacgtgaac gcaatcgcat tgaaaaccga tctcttggtc atattaccat
     2341 tcatctgaac aaagccgatg agaaatatgt cgaacttatc atagaaaacg acggcttccc
     2401 gattgcaccc gaaattcgcg aacatatatt tgaacccttc tttacgacca aacccatcgg
     2461 caaaggtacc ggactggggt taagcgctgt ttacggcatc attcgccgtc accacggaca
     2521 gatacgctgc gtgtcggaac cgttccagcc ggtgcgcttc attataatgt taccgtttgc
     2581 aataccgtca taataccggt ttttgatata tgtaattttc gcaaaattga tttttatcat
     2641 cccgactcct catcccgatc atagacaaat ctcccataac atgatagttt aaggcagagc
     2701 tgcaaaacag ctctcctttt agtttaaata aataactaaa tggttatgta actaattagt
     2761 ttttatgtac ttccactttg gagagatcgt atgaaatcta ttttacactc catcacccgt
     2821 atcctgacac tcttttatat actggctttt ataaatcagg cttgggctac cgacggatat
     2881 ttttcacatg gctatggtac caaacaaaaa agtatgggcg gtgtatccac cgcgttgacc
     2941 tttaatgcgt tttccgccat cacgaacccg gcgaacgccg tttatattca aaacaactgg
     3001 ggactgggta ttgctttctt taatcccaac cggcaatacg ccgtaagcgg ttcaccttcc
     3061 ggttttccca atacgtttcc gctcacaccg ggaacggtca aaagtaaaag tacgggattt
     3121 tttattccat ccctgagtta caatcgcctg atcaatgaaa aatccgcttt caattttgct
     3181 atttttggca atggcggaat gaataccgat tataaaacca atacgtttaa tacgaccaaa
     3241 cccacgggtg ttaatctatc acaacttttt gtcaatacga gttatgcata taaagtgacc
     3301 gacaaccatg cttttggcgt taccgcgatt ttcggatacc aatggttcga agccaaaggg
     3361 ctcacagcct tcagcggatt ttcaagatac aacaccaaac tcacggacaa taaatcggat
     3421 aaagcctacg gatacggcgc tcgtctgggt tatagcggac agatcacgcc gcagttgcgc
     3481 atcggcgcat cgtaccaaac gcgtatatgg atgaccgaat tcaacgacta caaaggcctc
     3541 tttgcggaaa agggtgattt tgatgtgccg gccacatgga atgccggatt agcatgggat
     3601 attaattccg catggacgct cgcggcagac cttactcaaa tctattatag ctccgtcaaa
     3661 tccgtcggca atccgatgaa accggctgat tttcaaaacg acattttact cggcgacaaa
     3721 aaaggcgccg gatttggctg gaacgatatg acggtttaca aaatcggttg cgaatatcgt
     3781 cacggcaacg attggtcggc ccgcgtgggt tacaactacg gcaaacaacc tatccccaca
     3841 agcgaagtga tgtttaacat cctcgcaccc ggcgtgatcg aacatcatct tacgtttggc
     3901 gccagcaaaa agatcggatc gaaatacgaa ttcagtttgg ctgtcatgcg cggtttttcc
     3961 aatgaagtca aaggcgccaa tccgatggaa gctccgggac aacaagcgat tgcactcaag
     4021 atgaatcaat gggaatttga aatcggttta ggtttctaat atttaacagg cgattacatc
     4081 atgttatcac gcagacgttt tttaaaaatc ggagccggat ttacagccgg tgcaaccatc
     4141 gcggccggcg ccgctcatac gttggctgat cgtttagccg cgttggaaaa accctccgga
     4201 caggtagaag tgataccgac gtattgtgat atttgttttt ggaaatgcgg tgttttggcg
     4261 catgtcaaag acggtcagct ttggaagttg gaaggtaatc cggaagatcc gcttagccgc
     4321 gggcgtctgt gcccgcgcgg cacaggcggg gtcggcgcac actacgaccc ggatcggctg
     4381 cgtacgcccc tgatccgccg cggtgaaaaa ggcgccgaag aatggacctc cgtcacatgg
     4441 gatgaagcgc tggacttcat cgccgaaaaa atgaaaaaaa ttaaagccga atacggcccg
     4501 gaatccgtgg cgctttttag ccatggtatc ggcggtaatt tttttaagca tacgatcaaa
     4561 gcctacggct cacccaatat cgcggcgcct tcgtttgccc aatgccgcgg tccgcgggat
     4621 gtcggatttc gcctcacctt cggcgaagat gtgagttcac ctgaacgcac tgacatccgc
     4681 aacgccaagt gcctcgtttt gatcggttcg catctcggcg aaaatatgca caacacgcag
     4741 gttcaggaat ttgccgaagc cgtagaccat cacgcgacga tcatcgtcgt ggatccgcgc
     4801 tattccgtcg ccgccagcaa agcgcagcat tatctcccga tcaaaccggg taccgatctc
     4861 gcactgatct tggcgtggat gcatgtcatc gtcaaagaaa atctttatga taaagcgtac
     4921 gtcgaacagt atggttttgg tttcgacgca tttgttgcgg cgttggaaac ttacacgccg
     4981 gagtgggcgt atcctgaaac cggcattgat ccggaaacga ttcgcaccac cgcccgcgta
     5041 atggcacaac acaaacccgc gactctcgtg catccgggac gtcacgtcac atggtatggg
     5101 aatgatgccc aacgcagccg cggcattgct ctgcttaatg cgctgctcgg cagctgggga
     5161 agcaaaggcg gtttttatac gccggtcagt atggatgtgc cggctttccc ttacccctct
     5221 tatcccaaat cggataaagg taaagtggat aaccccggtc ataaatatcc ttttgcgcac
     5281 gaaacgatca ccaccggcat acgcgaagcg acactcaccg gtaagcctta tccgatcaaa
     5341 ggttggtttg tgtacgcaac caatctgctg catgctttgc ctaacgagga agaaacaatc
     5401 aaggccattc agaatcttga tctgatggtc gtgattgatg ttattcccag cgaaatcgcc
     5461 ggctgggccg atgtgatatt accggagtcg gtgtatctcg agcggcacga cgatcttaat
     5521 gtcgaatggt tccgcgaccc gtttgtcgcc ctgcgccaac ccgtcgtgtc tgccccgcat
     5581 gaccaaaagc ccaactggtg gatggccaaa cgccttgccg aaaaactcgg gctcggtcat
     5641 tattatccgt ggacccatat cgaatcgtat tttgaaacgc ggctccgtca ggcaggattg
     5701 agttatgata cgctgaaaaa aaacggaatc ctcctcggcg aaaaaaaacc ggtttatttc
     5761 gacgaaggcg taccggccga gtttcccacg ccatccggaa aaatagaatt ttattcatcc
     5821 caactcgctg aagccggttt tgaaccggtg cccaaatatg tagccccgcc tcagccgcca
     5881 ccgggttcat ttcgactttt gtttggtcgt gcgccggtgc actcgtttag ccgcacgcat
     5941 accaaccccg tactgatgga tatgatgaat gaaaacgaac tttgggtcaa tgcggacatg
     6001 gcgttacgct acggtttaaa atccggcgat tatgttcgcc tcaaaaatca ggacggcgtg
     6061 gtcagcacta aaatcaaaat caaagcgacg gaacgcattc gcaccgactg tgtgtatatg
     6121 gtgcacggat ttgggcataa cgccaaagcg ctcaaacgcg ctttcggacg cggcgccagc
     6181 gatgcccagc ttatcacacg ctacgatacc gatcccttga tgggcggcac cggtatgaat
     6241 aataattttg tcactctgga aatggaggct taaccatggc gcgtctcgca atggtcatag
     6301 atacccgaaa atgcgtcgga tgcatggatt gtgtcgtggc ctgcaaaact gaaaatcagg
     6361 ttcccgaagg atacaa
//
LOCUS       DAOQGF010000160         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6062_length_6355_cov_5.127460, whole genome shotgun sequence.
ACCESSION   DAOQGF010000160 DAOQGF010000000
VERSION     DAOQGF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_6355_cov_5.127460"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK937_14640"
     CDS             complement(<1..523)
                     /locus_tag="PK937_14640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH33860.1"
                     /translation="MQPFLEKKNVPPYSILADFYDGMMEHVDYRTWSSFIHQIFERYG
                     SGISTVCEFACGTGNFAAAMAAFPYTMICSDRSETMIMTARKKPVAQSGKILFFTGDM
                     LSFKPSVECEAALCLYDSVNYLMKAEDILNFFNHVSECTTGRGIFVFDVCTEKNSLDH
                     FDQRYELDRTHHYK"
     gene            complement(507..1169)
                     /locus_tag="PK937_14645"
     CDS             complement(507..1169)
                     /locus_tag="PK937_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNH33861.1"
                     /translation="MPNLRLQEFSAVLFDFDGVLMDSIGDHHRSWNSVFSEYGVSISW
                     DEFCLLEGQSLFVIAEQLCMNHGVDIKHGQEIGKKKNNLYLSTAKPKLYDGAEDLIEW
                     FRKQGLKTGLVTGAHRDRFYRTVVPDFTKRFEVIITADDVMKTKPDPEPYTTAATRLS
                     VSPERCIVIENAPLGVTAAKKAKMVCIALTTTLDQKYLNEADIVIPDLFSLKAMAHAA
                     FS"
     gene            complement(1170..1877)
                     /locus_tag="PK937_14650"
     CDS             complement(1170..1877)
                     /locus_tag="PK937_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNH33862.1"
                     /translation="MISVIIPVLNEGQWITYCIEQLKHQNGAFEVIVSDGGSTDGTLE
                     RVSELKNLSFPCTVVSGDKGRANQLNRGAQAAKGEVLLFLHVDCELPPNAFKSVEQFL
                     SDPQCIGGGFQKKYNPENILMRLYRIMINTIRTLWLRNLVGTNAIFVRSRVFHQLGGF
                     KQVSLLEDVIFCDAMKKAGRMVIIKDPVIVSSRRYFSSGIVKRIGIAAEVIIRFRVLG
                     ASPEELEMRYHRRMKKR"
     gene            complement(1874..2998)
                     /locus_tag="PK937_14655"
     CDS             complement(1874..2998)
                     /locus_tag="PK937_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HNH33863.1"
                     /translation="MIDPKKKAPLYVPPVKNIFSPQEILYLSDMRLSDLEAIRLVLNG
                     DSVIDWFRLDFHTPDQVQLFLQVNGYDLTNVQDRKRIEKIHREAVEYLDSKYPHRIPK
                     EIRPESATIEQLLYLASQSARKNKHQFYACMVLKIMHTINHLDARELFHRCQMRASDL
                     YFAVERKVEHAVTTMKNNEFHIVDFYGSHKDKMSMVTKLMAKKENHAAAIYDRIRFRI
                     ITQTLEDILPVLYFLGRTICPFNYVIPGSSHNNLIAFAELVEMFPNLRSYAKFLRRKD
                     THGFDPDEVSKENKFSSTSYRSINIVMDIPIRVDDFLYTPDFSEFGRIIFVPVEIQIM
                     DQMTYQNNEIGEGSHSKYKQRQMDAVNQRLGLQQNGNSPG"
     gene            complement(2995..3846)
                     /locus_tag="PK937_14660"
     CDS             complement(2995..3846)
                     /locus_tag="PK937_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNH33864.1"
                     /translation="MIEKTSELWADLHMHSYYSDGILSPEAIVKYAAKIGLSCIAITD
                     HDTVKAVDEATEAGNKHNVEVLTGIELSTTWDGRDIHVLGYCFDHTDAALDVQLQLYR
                     DERRHRAIRIIDRLQKQGMEITIEEVEKKAGRGVIGRPHIADVMLSKKMAYNINDVFA
                     RFLGPDGTAYEDKYKISPRFAVELIEKAGGVAVLAHPTLNMKETHVHDMIKAGIHGIE
                     CIHPKHTADSTAYYRRLTVQYGLEESGGSDCHGRGQVPLIGQYRVPYNVVQRLKDRAQ
                     ALRKVNA"
     gene            complement(3853..4944)
                     /locus_tag="PK937_14665"
     CDS             complement(3853..4944)
                     /locus_tag="PK937_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH33865.1"
                     /translation="MQKKVLIVDDEEDLTWSISKNLSKDKDSFDLICVNNAQEALNVL
                     SQLPVDLVVSDIKMPGMSGLELLVKIKETYSTTKVIIMTAFGSAEVQREATDRGSLYY
                     IEKPFEIEELRTLIHRALTEKKGFDGKVSDFQLSDLIQMNILGRMKAALVVNKDEEKG
                     TLYFSDGNIVHAECGSIIGEEAFYKILAWENGRFEFRKGEDPEKETIDKGWQSLLLEG
                     MRRKDEYSPETKTQIKEESRQEMLEKIKTSIAEFIKIKGVDLVAVIDDAGFSRASLFN
                     EKKEERMDITLLSTIIPPGLEYLLKVDTELKGEGLRFITLDFNKKTIFMAPVPGRKEW
                     LFVLCEPEVNLGGLRMAFKKQAPLLSELL"
     gene            complement(4958..>6355)
                     /locus_tag="PK937_14670"
     CDS             complement(4958..>6355)
                     /locus_tag="PK937_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNH33866.1"
                     /translation="RDERIPAAIYALTPNPLRAAICTPIYIRGQLMGVLMIINKKTQM
                     GFEHGDLSLLMTVSGQIALAMENMQLFNKLSDFNRNLQDQIRQATHALVDLKNFNESI
                     LHNVSAGIITADFSGRILFVNRAAKSILGFSDNELLDHKMETIFGREEASRMLHPTVE
                     NESAPGNAEVQVRQKDGTRLFLGFTTTMRYDVDHNMVGYIISFKDISLVKSMHETIMR
                     MDRLAASGALTSAIAHEIRNPLAGIKTIAQALQKELPPDDPRHEYVYRIIKQINRLND
                     LLKAFFTYAKPVRPEKKACALPELIQEIKALLKARAETDHIEIHEAYDEKLAPIEVDE
                     NQFSQVMLNLMMNAMDAIGQGGRIAIRAQSVQRTPLNNYDLPITMAEIRVSDTGKGIP
                     QEQVRSIFDPFFTTKPGGIGLGLSIVYRIVQEHGGEIKVESAPGKGTTFIMHIPCAEK
                     SKSILENRHTLTTTI"
BASE COUNT         1655 a         1500 c         1363 g         1837 t
ORIGIN      
        1 gtttgtaatg atgtgttcga tccagttcat accgctgatc gaagtgatcc agtgaatttt
       61 tttcagtaca gacatcaaaa acaaaaatac cacggccggt tgtacattcg gaaacatgat
      121 taaaaaaatt cagaatatct tccgctttca tcagataatt gacactatca tataggcaaa
      181 gcgctgcttc gcattcgaca gaaggtttga aagaaagcat atcgcccgta aaaaaaagta
      241 tcttacctga ttgagcaacg ggttttttac gtgccgtcat gatcatggtt tcagagcgat
      301 cgctgcatat catcgtataa ggaaacgcgg ccatcgctgc ggcaaaattt ccggttccac
      361 aggcaaattc acaaacggtt gatataccgg acccgtagcg ttcgaagatc tgatgaatga
      421 atgacgacca cgtgcggtaa tccacatgct ccatcatacc gtcataaaaa tctgcaagta
      481 tagaatacgg aggtacattc tttttttcaa gaaaaggctg catgcgccat cgctttcaga
      541 gaaaataaat ccgggattac aatatccgct tcattcaaat atttttgatc tagggtcgtc
      601 gtcagtgcga tacacaccat cttcgctttt ttggctgctg ttacgcccaa aggtgcgttt
      661 tctatcacaa tgcaccgttc cggtgatacc gaaagtctcg tcgccgctgt cgtgtagggc
      721 tccggatcag gtttggtttt cataacgtca tcagccgtta tgatgacttc aaatcgtttt
      781 gtaaaatccg gcaccactgt tctataaaaa cggtcccgat gcgctccggt aaccaatccg
      841 gttttcagac cttgtttgcg aaaccattcg atcaaatcct cagccccatc gtaaagctta
      901 ggttttgccg tactcaggta taaattattc ttcttttttc caatctcctg accgtgttta
      961 atatccacgc cgtggttcat acataattgt tcggctatta caaaaaggct ttggccttcg
     1021 agtaaacaaa attcgtccca gcttatggaa acgccgtatt cagaaaaaac actgttccaa
     1081 gagcgatggt gatcgccgat gctatccata agcactccgt caaaatcaaa gagtacggct
     1141 gaaaattcct gtaaacgtaa gtttggcatc tatcgttttt tcattctgcg atgataacgc
     1201 atttctaatt cttccggtga agcgcccagc acacgaaagc gaatgatcac ttccgcagcg
     1261 ataccaatgc gtttgacgat gccggatgaa aaatatcggc gcgacgaaac gataaccgga
     1321 tcttttatga tgaccattcg cccggctttt ttcatcgcat cacaaaatat cacatcttcc
     1381 aataaggaca cttgtttaaa tccgccaagc tgatgaaata cgcgtgatcg gacaaagatc
     1441 gcattggtgc caacgagatt acgcaaccaa agtgtacgga tagtattgat catgatacgg
     1501 tacaaccgca tgagtatatt ttcgggatta tatttttttt gaaatccgcc gccgatgcat
     1561 tggggatcgc tcagaaattg ttcaacagat ttaaacgcat tcggaggaag ttcgcaatct
     1621 acatgcaaaa agagcagcac ctcaccttta gcggcttggg ctccccgatt aagttggttc
     1681 gctcgccctt tatcacccga tacaacggtg cacggaaaag ataaattttt caattcacta
     1741 acacgttcga gcgttccatc tgtactgcca ccgtcggaaa cgataacttc gaatgcgccg
     1801 ttctgatgct tcagttgttc aatacagtag gttatccact ggccttcatt gaggacaggt
     1861 atgatgacag agatcatccc ggagagtttc cgttttgttg gagaccaagg cgttgattga
     1921 cagcatccat ttgtcgctgt ttgtatttac tatggctgcc ctcaccgatc tcattattct
     1981 gatacgtcat ctggtccatg atctgtattt cgaccggcac aaaaataata cggccaaatt
     2041 cggaaaaatc cggtgtgtat aaaaaatcat ccacacgtat cggtatatcc ataacgatat
     2101 tgatggaacg atagcttgtg cttgaaaatt tattttcttt ggatacttca tccggatcga
     2161 aaccatgcgt atcttttcgt ctgaggaatt ttgcgtacga tcgcagattg ggaaacatct
     2221 caacgagctc cgcaaaggca atcagattat tatgcgaact tcccggaata acataattaa
     2281 acggacaaat cgtgcgacct aaaaaataca ataccggcag tatgtcttcg agcgtctgag
     2341 taatgatacg aaagcggata cgatcataga tagccgccgc atgattttct ttcttggcca
     2401 tcagtttggt gaccatgctc atcttgtctt tatggcttcc gtaaaaatca acgatatgaa
     2461 attcgttatt tttcatcgtc gttacggcat gttccacttt tcgttcgacc gcaaaataaa
     2521 gatccgaagc ccgcatctgg caccggtgaa aaagttcgcg cgcatccaga tggttgatcg
     2581 tatgcatgat cttgaggacc atacacgcat aaaattgatg tttatttttt cgtgcggact
     2641 gtgaagcgag atacaaaagt tgttcgatgg tggccgattc cggtcgaatc tctttgggta
     2701 tgcgatgcgg gtatttactg tcgagatatt cgaccgcttc gcgatgaatt ttttcgatac
     2761 gtttgcggtc ttgcacattg gtaaggtcgt acccgttgac ctgtaaaaaa agctgaacct
     2821 gatccggtgt atgaaaatcc aggcgaaacc aatcaatcac ggaatctccg ttgagcacca
     2881 accgaatggc ttcgagatcg ctcaagcgca tatcgctgag atagagtatc tcctgcggag
     2941 agaaaatgtt tttcaccggc ggaacgtaca atggcgcttt tttcttggga tcaatcatgc
     3001 gtttactttg cgcaaagctt gtgcgcgatc cttgagtcgc tgaacgacgt tatacgggac
     3061 acggtactga cctatgagcg gcacttgccc tcgcccgtgg caatcggaac cgccgctttc
     3121 ttcgagcccg tattgtacgg taaggcgccg gtaatatgcg gtcgaatcgg ccgtatgttt
     3181 gggatgaatg cactcgattc cgtggatacc ggccttgatc atatcatgga catgcgtttc
     3241 tttcatatta agcgtaggat gcgccaaaac ggctacaccc ccggcctttt cgatgagctc
     3301 gacggcaaaa cggggtgaaa ttttatattt atcctcatag gccgtaccgt ccgggccgag
     3361 aaaccgcgca aaaacatcgt tgatattgta cgccattttt ttggatagca tcacatcggc
     3421 aatatgcggt ctgccgatca ctccgcgacc ggctttcttt tcgacctctt cgatcgtaat
     3481 ctccatgcct tgtttttgaa gacggtcaat aatacgtatg gcccggtggc gccgctcatc
     3541 acggtaaagc tgcagttgta catccaatgc cgcatcggtg tgatcaaaac aatatcccaa
     3601 aacgtgaata tcgcgtccat cccaagtcgt actgagttct atgccggtca ggacttccac
     3661 attatgttta ttccccgctt ctgtcgcttc atccacggcc tttaccgtgt catgatcggt
     3721 gatcgctata cagctcaggc cgattttagc ggcgtacttt acgatggctt ccggcgataa
     3781 tatgccatcc gaataatagg aatgcatatg cagatcggcc caaagttcgg acgttttttc
     3841 gatcataaat ccttagagta attccgataa tagcggcgct tgttttttaa aagccatccg
     3901 aagtccgccg agattgactt ccggttcgca taagacaaat aaccattctt tgcgaccggg
     3961 cacgggcgcc atgaaaatgg tttttttatt gaaatcaaga gtgataaaac gaagtccttc
     4021 gcctttcagt tccgtatcca ctttcagaag atattccaag ccgggcggta tgatcgtact
     4081 cagaagggtg atatccatac gctcttcttt tttttcatta aaaagcgatg ctctcgaaaa
     4141 tccggcatca tcaatcactg ctacgagatc cacgcctttg attttgataa actccgctat
     4201 agatgtcttt attttttcga gcatctcctg acgcgattct tctttgatct gcgttttcgt
     4261 ctcgggcgaa tattcgtctt tacggcgcat accttcaagt aaaagacttt gccacccttt
     4321 atctatagtc tctttttccg gatcttcacc tttacgaaat tcaaaacgcc cattctccca
     4381 tgccaatatt ttataaaacg cttcttctcc gataatcgaa ccgcattcgg catggacgat
     4441 atttccgtcg ctaaaatata atgttccctt ctcttcatct ttgtttacca ctaaggccgc
     4501 tttcatacgc ccaagtatat tcatttggat aagatcggac aactgaaagt cggaaacttt
     4561 accgtcaaat ccttttttct ccgtcagtgc acggtgtatg agcgtacgca gttcttcgat
     4621 ttcaaacggt ttttcgatgt aatacaacga accgcgatcc gtagcttcac gttgtacttc
     4681 ggccgaacca aaggctgtca taatgataac cttggttgtc gaatacgttt ctttgatttt
     4741 tacaagcagt tccaacccgg acatacccgg cattttgatg tcgcttacta ccagatccac
     4801 aggtaattgc gaaagaacat taagcgcctc ttgagcgtta ttgacacaga tcaggtcaaa
     4861 actatccttg tctttagaca gatttttgga aatgctccac gtaaggtctt cttcgtcgtc
     4921 aactatgagt actttttttt gcatatagat ttaacggtta gatggttgtt gtcaatgtat
     4981 gccggttttc caatatggat tttgattttt ctgcacaagg aatgtgcatg atgaatgttg
     5041 tgcctttacc cggtgcactt tccactttga tttcacctcc gtgctcctgc acgatacgat
     5101 acacgatcga aagacctaac ccgataccac cgggtttagt cgtaaaaaaa ggatcaaaaa
     5161 tggaacgaac ttgctcttga ggaatacctt tccccgtatc gctgacgcgt atttccgcca
     5221 tggttatcgg taaatcatag ttattcagcg gtgtacgctg aacggattga gcacggatcg
     5281 cgatgcgccc tccttgaccg atcgcatcca tagcgttcat cataagatta agcatgactt
     5341 gcgaaaattg attttcatcc acctcgatgg gcgcgagttt ttcgtcatac gcttcgtgta
     5401 tttcgatgtg atccgtctcg gcacgcgctt tcaaaagggc cttgatttct tgtatcagtt
     5461 ccggcagcgc acaggctttt ttctccgggc gcacgggttt ggcataggtg aaaaaagctt
     5521 taagcaaatc gttgagccgg ttgatttgtt tgataatgcg ataaacatat tcatggcgcg
     5581 gatcatcggg cggcaattct ttttgaaggg cctgtgcgat ggttttaatg cccgccaacg
     5641 ggttgcgaat ttcatgtgca atagcgctgg tcaatgcgcc cgatgcggcg agacgatcca
     5701 ttcgcatgat cgtctcatgc atggatttga ccaaagaaat atctttgaaa ctgataatat
     5761 aacccaccat gttgtggtca acgtcgtaac gcatagtggt cgtaaaacct aaaaaaagcc
     5821 gtgtgccgtc tttttgtcga acctgcacct ccgcattgcc cggcgcggat tcgttttcta
     5881 ccgtcggatg aagcatgcgt gaggcctctt cgcgaccaaa aattgtttcc attttgtgat
     5941 caagcaattc gttgtcggaa aatcctaaaa tggatttggc ggcacgattg acaaataaaa
     6001 tgcgcccgga aaaatcagcc gtgatgatac cggcgctgac attgtgcaaa atactctcat
     6061 taaaattttt taaatccacc aacgcatgcg tcgcctgtcg aatttgatcc tgcaagttac
     6121 gattgaaatc cgacaactta ttaaaaagct gcatattctc catcgctaaa gcgatttgac
     6181 ccgataccgt catgagcaat gagagatcgc cgtgttcaaa tcccatctgt gtttttttat
     6241 tgatgatcat cagtaccccc atgagctgac cgcgtatgta tatgggtgta catatcgctg
     6301 cacgcaacgg attgggcgtc agcgcataaa tcgccgcggg tattctttca tcgcg
//
LOCUS       DAOQGF010000161         6195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6299_length_6195_cov_4.448860, whole genome shotgun sequence.
ACCESSION   DAOQGF010000161 DAOQGF010000000
VERSION     DAOQGF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_6195_cov_4.448860"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..725)
                     /gene="plsY"
                     /locus_tag="PK937_14675"
     CDS             complement(63..725)
                     /gene="plsY"
                     /locus_tag="PK937_14675"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNH33867.1"
                     /translation="MISFLAILIIAYLLGSISPSILISRWIKGVDIRTYGSGNAGMTN
                     AIRLLGGRWGAVVAIIDILKGLISSLWLTDALYSAFDATPLEITLVRILAAIAAVAGH
                     IWTIFFGFKGGKGVLTMAGAVLGVAPLPVGICFATFILIFALTRYVSLGSIIGSWVFP
                     IVVFIQRFVLQQSVSDYLLGFSIFVMVLITFTHRENIRRLMRGEEKKFGAAKSATPNQ
                     AV"
     gene            complement(890..1309)
                     /locus_tag="PK937_14680"
     CDS             complement(890..1309)
                     /locus_tag="PK937_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH33868.1"
                     /translation="MRILIIDDSELIRERLKTLITEECRDVFIETEARAAQALESVST
                     IRPDVVILDIKMRDGSGVGVLTQIKNLSSPPIVIMFTQHPYPQFKSRCIFYGADYFLD
                     KSTDMDQIPAIVVNLGKTQRLNGRISDSPGGFRHTQI"
     gene            complement(1618..1983)
                     /locus_tag="PK937_14685"
     CDS             complement(1618..1983)
                     /locus_tag="PK937_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH33869.1"
                     /translation="MRILIADDSALVYERLSSLIHELHKNLELVGHAETAPDTIRQAI
                     MQKPDVVILDIKMPGGNGIEVLKQLKSEPKPPVIIMFTQYPYPQFRDRCLQYGADYFF
                     DKTSEFDRIGDVLEKIAMN"
     gene            complement(2349..2993)
                     /locus_tag="PK937_14690"
     CDS             complement(2349..2993)
                     /locus_tag="PK937_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH33870.1"
                     /translation="MDHSASIERILIADDHAIVRKGLAETVLERYPRCHITHASNADE
                     LLGCIQAEIFDVALMDISMPGVTGLEALKQVRMIRPQLPVLILTAYPESQYAERAIRL
                     GAMGYINKSVASDELVEAIRTVKAGKKFITKAVAMILAEKDTGADEPHTNLSDREYEV
                     MRLIAQGLSPKEIGEALSISPKTVNSMRDRILEKMNLTSNAEIIRYAIQKKLVE"
     gene            complement(3002..>6195)
                     /locus_tag="PK937_14695"
     CDS             complement(3002..>6195)
                     /locus_tag="PK937_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH33871.1"
                     /translation="YTLKDGLSQNLVTCALQTRDGFMWFGTREGLNRFDGYEFLIFQH
                     EETDSFSISHNYVTALLEDYDGRIWVGTEEGLNRFDPITGRFEKFFYDPMQPSSISNN
                     NINVLHQDSFGTLWIGTHLGLNKLRQTNPLGFEQFLHDPNDKKSLPSNLIRAICQVHN
                     ADGTPEKKLWIGTTGGGLSLMDTDFNTFSSPYEDLFVPRKFNFSTVWDVYEDPVDKGN
                     TLWVCSPQGLGKINRTTKQWQYFTFENKNPNSISHNRVRRILRDSYGRLWVGTTEGGI
                     NLMTDDTFLNFRNTPNQPSLFTDEYIYDIYQSSDGIVWICTYSNGVTQITGMEPRFVH
                     YKKNPFDPTSLIHNVVRSFTESRDSSIWISTDDGISRWDRDADRFEHLRYIPDNPNAL
                     NDRQIHRVYEDRSGKIWIGTHQNGLQCYDPERKTMKSFRRSTTQGQFTATRVYDILED
                     ENGFIWIATYRGGLIRYDPQINQFKTYGIDKTGDQNIREEMLTVLHRDNSGKLWIGTQ
                     QRGLMCFDRQSEQFTTFSKNTANTQTISDNHVYSIYQDRLDYLWIGTANGLNRFDPRT
                     HKFQRITRKDGLPNNVIYGILGDENDNLWISSNRGIIRMQTKSDTSRSFVSYTAEDGL
                     QANQFEPNACLRTRAGEFLFGGVNGFNIFHPRHITPTGQAPRLVLTDFRLFDKPTVLD
                     TSIPYKHDLHLDYDQNFFSFSFSALNFFVPEKNQYAYKLDGIDENWNFVTGKNRIVKY
                     TDIDPGNYTLLVRASNHDQIWNNDGLRFPIVIRPPYWETWWFRTILFAALIGVLIFAY
                     SARVRNIEAMNRRLEGIVIERTAALNQKKDELQHAHDELEKRVEERTKQLRDLSDHLV
                     RVREEERTRISREVHDELGQQLTALKMDLSWIQKRLPEDSSLKEKSNTMSSLIDATVK
                     SVQRIASELRPGLLDDLGVSAAIEWQSSEFEKRTGIQCRCYLEQGDTAVTNEQGTALF
                     RIFQETLTNVMRHAEATEVVIEFFAEHEHLHLIIRDNGKGITKVQLNSPHSLGLMGMR
                     ERIQHLQGEITISGEQGKGTVVHVSLPIINPIS"
BASE COUNT         1776 a         1298 c         1347 g         1774 t
ORIGIN      
        1 taagggctag cacggcatac caaaaaaacc gataacggag aactgtgtaa atcgcctgca
       61 cgtcagacgg cctgattagg tgtggcggat ttagccgcgc caaatttttt ttcttcaccc
      121 cgcatcaaac gccgaatgtt ttcgcggtga gtaaatgtga tgagtaccat cacaaagatg
      181 ctgaatccca aaagataatc ggaaacagat tgttgtaata caaaacgctg gataaaaaca
      241 acgattggaa aaacccatga accgatgatt gagcccaagg aaacatagcg tgtgagcgca
      301 aaaatcagaa taaatgttgc gaaacaaata ccgacaggta acggtgccac acccaatact
      361 gcgccggcca tggttaaaac gccttttccg cctttgaatc cgaaaaatat ggtccatata
      421 tgcccggcaa cagcggcgat agccgctaag attcgtacca gagtgatctc taacggtgtc
      481 gcatcaaatg cagagtataa ggcatcagta agccataacg atgaaatcag acctttgaga
      541 atatcaataa tggctacaac agctccccag cgtcccccga gtaatcggat cgcattggtc
      601 atacctgcat tgccgctacc gtaggtgcga atatccacac cttttatcca gcgggatatt
      661 agaatactcg gactgatcga gcctaggaga taagctatta taagtattgc taagaatgaa
      721 atcatgatgt tctctatgtt actgaacggc gcatgatacg tattaatgta aaaaaattca
      781 atgttatatt tgaaatgtgt gagatactct aaatacatca aaccctcatg ctgaccaaac
      841 atgagggttt gttattaaga aaaggaggag gattcaaaac gggtacagtc taaatctgtg
      901 tatgccgaaa gccgccgggc gaatcactga tacgaccatt caagcgttga gtttttccca
      961 ggttcacaac gattgcaggg atttgatcca tgtccgtaga tttatcaaga aaataatcgg
     1021 caccataaaa tatgcatcgg cttttaaatt gaggatacgg atgttgcgta aacatgataa
     1081 caatcggggg tgaagacagg tttttgatct gagtcaaaac accgacgccg ctgccatccc
     1141 gcattttgat gtccagtatg acaacgtcag gacgtattgt gcttacggat tctaacgctt
     1201 gtgcggcacg ggcttcagtt tctataaaaa catcccggca ttcctccgta atcaacgttt
     1261 ttaaacgctc gcgtataagc tccgaatcat ctatgatcag tatgcgcata tacttattcc
     1321 gtcaaaaata aaaatttagt gctcgaacga tcgtgcaaga aaatatataa aaccgagcag
     1381 aatgaatata atacatcacg tgatcatact agaacacaat gtctgattag atattgattc
     1441 ctacataaga taatcaaaat taagttattt gtctgtaggt aaatggctga aaatcgtgta
     1501 ggtaatttac ctacaaatct tgatgaggca ggattaagtt taaattaatc aaagcaaggt
     1561 aaaacaaaac aacccgcgat gagttcggag gttgtttatg ttaaaatcaa aatgggatca
     1621 attcatcgcg attttttcga ggacatcacc gatacgatca aattcgctgg ttttgtcaaa
     1681 aaaatagtcg gctccatatt ggaggcaacg atccctaaac tgtggataag ggtactgtgt
     1741 aaacatgata ataaccggcg gttttggctc ggattttagt tgtttaagga cttcgatgcc
     1801 gttccctcca ggcatcttaa tgtccaaaat tactacatcc ggtttttgca tgatagcttg
     1861 tcgaattgta tcaggcgccg tctccgcatg accgactaat tccaaatttt tatgcaactc
     1921 atgtattaac gaagaaagtc gttcatacac cagcgctgaa tcatctgcaa tcagtattcg
     1981 cacaatgccc cctttctctt tttgtccccc aaaaagagac ttttagtttt tacgataata
     2041 gacccaagta acgcttgaaa tataacgttc gaaatcctga aagtatgtag gtatttttct
     2101 gaaaatcttg taggtaaatt acctacaaga tttatttaaa ctaaacattt acaatataaa
     2161 catataactg tttaaattta ttttaagggt tatataaaac tgctttgatc caatccactc
     2221 aggtgttcga tcattaggaa atgaaatgtg aatcaaggag aatgaaaaaa attagttggt
     2281 attcagagga tcagcggagg caaacaggta ttcaatacat acaaaaaata aaagttataa
     2341 aatagaactt actcgactaa ttttttttga atggcgtagc ggataatttc ggcattagat
     2401 gttagattca ttttttcaag aatacggtct cgcatcgaat taaccgtttt ggggctgatg
     2461 ctgagtgctt caccgatctc tttgggtgac aatccttgtg cgatcaagcg cataacctcg
     2521 tattcacgat ccgatagatt ggtatggggt tcatccgctc cggtatcttt ttcggctaag
     2581 atcatggcta cggctttggt aataaatttt tttccggcct ttaccgtacg aatcgcttcg
     2641 accagctcgt cggatgccac cgatttattg atatagccca tagcgcctag gcgaatcgca
     2701 cgttcggcgt attggctttc cggataagcc gtcagtatca gaaccggtaa ttgaggtctt
     2761 atcatacgca cctgtttgag tgcttctaat cccgtgacac ccggcatcga aatgtccatc
     2821 aacgcgacat caaatatttc cgcctgaatg cagccaagta attcgtcggc attggacgcg
     2881 tgtgtgatgt ggcaacgcgg ataacgttct agtaccgttt cggcaaggcc tttgcgaaca
     2941 atagcgtgat catcagctat aagtatgcgt tcgatggaag cagagtgatc cataaataat
     3001 tttaagaaat gggattgata attggcagcg atacatgcac tacggtaccc ttgccttgtt
     3061 cgccggagat cgtgatttcg ccttgtaaat gttggatacg ttcacgcatg cccatgaggc
     3121 ccagcgaatg ggggctattg agttgtactt tggtaatacc ttttccgttg tctcgaatga
     3181 tgagatgaag atgttcatgt tcggcgaaaa actcaataac gacttccgta gcttcggcat
     3241 ggcgcatcac gttcgtcagt gtttcctgaa aaatacgaaa cagtgccgtg ccctgttcgt
     3301 tggtaactgc tgtgtcgcct tgttccagat agcaacgaca ttggatgccc gtacgttttt
     3361 caaattcgga cgattgccat tcgatagcag ccgaaacgcc aagatcgtcc agcaatccag
     3421 gacgtaattc gctggcaatt cgctggacgc ttttgacggt ggcatcgatg agtgaactca
     3481 tagtgttaga tttttctttg agggatgagt cttctgggag gcgcttttgt atccaagaaa
     3541 gatccatttt cagagcggtc agttgttgac ccagttcatc atgcacttca cgcgaaatcc
     3601 gcgtacgttc ttcttcacgg actcgaacca agtgatcgga gaggtcgcgc agctgtttag
     3661 tgcgttcttc gaccctcttt tccaattcgt catgcgcatg ctgcaattca tctttcttct
     3721 gatttaaagc tgccgtgcgt tcgattacga taccttcaag tctgcggttc attgcttcaa
     3781 tattgcgtac acgagcggaa taagcgaata ttaaaactcc aatcagcgcg gcaaataaga
     3841 tcgttcggaa ccaccaagtt tcccaatacg gcggacggat tacaatcgga aaacgaaggc
     3901 cgtcgttatt ccaaatctga tcgtgattgg aagcgcgtac caggagcgtg taatttcccg
     3961 gatcaatgtc tgtatatttt acgatacgat tttttcctgt tacaaaattc caattttcat
     4021 caataccatc cagtttgtag gcgtattgat ttttttcggg gacaaaaaaa ttgagtgcgg
     4081 aaaaggaaaa actaaaaaaa ttctgatcat aatctaaatg aagatcatgc ttgtaaggga
     4141 tactcgtgtc caatacagtt ggtttgtcga aaagcctgaa atctgtcagt acaagacgcg
     4201 gcgcttgtcc ggttggtgtg atatggcgcg ggtgaaagat attaaagcca ttgacgccgc
     4261 cgaaaagaaa ctcaccggca cgtgtacgca ggcaagcatt gggttcaaat tgattggcct
     4321 gtagcccgtc ctctgctgta tagctgacga aactacgaga cgtatcggat tttgtttgca
     4381 ttcgaataat accgcggttg ctgcttatcc ataaattgtc attttcatcg cctagaatac
     4441 cgtaaataac gttgttaggt agtccgtctt tacgcgtgat gcgctgaaac ttatgagtac
     4501 ggggatcaaa acgatttaac ccattagccg taccgatcca taagtaatct aatctatcct
     4561 gataaatgct atacacatga ttatccgaaa tggtttgcgt gtttgccgtg tttttggaga
     4621 atgtcgtaaa ctgttcggat tgccgatcaa aacacattag tcctctttgt tgtgtaccga
     4681 tccataattt cccggagtta tctctatgga gcacggtcaa catttcttca cgaatatttt
     4741 ggtctcctgt tttgtcaatg ccataggtct taaactgatt gatttgcgga tcatatcgaa
     4801 taagtccgcc gcgataggtt gcaatccaaa tgaatccatt ttcgtcttcg agaatgtcgt
     4861 atacgcgggt cgctgtaaat tgaccttgtg tcgtcgaacg ccgaaaggac ttcatggttt
     4921 tacgttccgg atcataacat tgcaggccgt tctgatgcgt accaatccat attttgccgg
     4981 aacgatcttc gtaaacgcga tgaatctgtc ggtcatttaa tgcgtttggg ttatccggta
     5041 tatagcgcag gtgttcaaaa cgatctgcat ctctgtccca tcgggagatg ccatcatcgg
     5101 tgctgatcca gatcgaactg tcacggcttt cagtgaaact gcgtaccaca ttatgaatta
     5161 agctggttgg gtcgaacgga ttttttttgt agtgaacaaa acgcggttcc ataccggtga
     5221 tttgagttac cccgttggag tacgtgcaaa tccagacgat accgtcggaa gattgatata
     5281 tgtcgtatat atactcatcg gtaaaaagcg atggttgatt tggcgtgttt ctgaagttga
     5341 ggaatgtatc atcggtcatc aaattgatac caccttcagt cgttccgacc cacaaccgtc
     5401 catacgaatc gcgcaaaatc cgacgtactc gattgtggct tatgctgttc ggatttttgt
     5461 tttcaaatgt aaaatactgc cattgttttg tagtacggtt aattttgcct aatccttgtg
     5521 gagaacaaac ccataaagtg ttgcctttat ccaccggatc ttcatacaca tcccaaaccg
     5581 tactgaaatt aaatttacgg ggtacgaaca gatcttcata aggacttgaa aatgtattga
     5641 aatccgtatc cattagactg agaccaccac cggtagtgcc gatccaaagt tttttttcag
     5701 gtgttccatc agcattatga acttggcaaa tcgcacgaat taaattggaa ggcaaacttt
     5761 ttttgtcgtt gggatcgtgt agaaactgct caaaacccaa tgggttggtt tggcgtaatt
     5821 tattgagtcc taggtgcgta ccaatccata atgtgccgaa actatcttga tgcaatacat
     5881 taatattatt attgctgatg gatgagggtt gcatcggatc ataaaaaaac ttttcaaaac
     5941 gacccgtgat gggatcaaaa cggttgagcc cttcttccgt accgacccaa atacgtccgt
     6001 cataatcttc aagcaatgcc gtgacataat tgtgggaaat ggaaaaactg tcggtttctt
     6061 cgtgctgaaa gataaggaat tcatacccgt caaagcggtt gagaccttcg cgagtaccaa
     6121 accacataaa tccgtccctg gtctgaagcg cgcaggttac cagattttga gataatccat
     6181 ctttgagcgt atagg
//
LOCUS       DAOQGF010000162         6003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6591_length_6003_cov_6.276059, whole genome shotgun sequence.
ACCESSION   DAOQGF010000162 DAOQGF010000000
VERSION     DAOQGF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6591_length_6003_cov_6.276059"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK937_14700"
     CDS             complement(<1..319)
                     /locus_tag="PK937_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33872.1"
                     /translation="MRKKWLIAVGVGIAAIGILGFTGPGQNAYKSIQKLQYVLSIVTQ
                     TYVEDVNSDKLVEDAIVGALKGLDPHSIYIPPKEMKTVKEEFQGNFDGIGSQLDVING
                     IPTI"
     gene            complement(333..2522)
                     /locus_tag="PK937_14705"
     CDS             complement(333..2522)
                     /locus_tag="PK937_14705"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNH33873.1"
                     /translation="MKKIRLILGIHNHQPVGNFDFVLDKAYQNAYRPFLDMLRDFPGV
                     RVGMHTSGILYEWLDKQYPEYSIQLRTLTDRGQIELIGGGFYEPILPIIPHRDQVGQI
                     QRLQKYLLQRYEQAAQGMWLAERVWEPHLPSVLSQVGVRYVILDDTHFLHAGLSVDDL
                     SGYYITEDQGATLALFPISQQLRYLIPFEAPEKTIDYLRDLAEKNENAVAVFADDGEK
                     FGIWPDTYQHVYENGWLQRFFEVLEANRDWIEVCHFSDVLRSVPPKGKIYLPTASYSE
                     MLHWALPLSGYRNYELFQSKLKEEKIAESFNAFVRGGFWRNFFSKYAESNRMHKKMLR
                     VSARLAKVAEAIPKDDKVKQSLLESAQNHLWASQCNCPYWHGVFGGIYLNNIRFAVYR
                     HMLNAEVLTDQLDAYTQGSAYRLEETDFDTNGKNEILFESPKLNAYLDPHHGATLFEI
                     DYKEIPMHVTDILTRREEGYHHQLAEASITEPSKKNTESDDSIASIHDRVLSKEPDLH
                     RHLIYDQYERRSFRDHVLTDNCDLEAWQNQTYSVYADLADASYDVERKEYERYVEITC
                     LRETELEMAPMRIEKKYKIFSDDPRIEVHYRWHNRGKTEVKAKFGTEINLTLLAGQAD
                     DRFYAVGGRKWPDGAMQSSGTFAGIRSLNMIDGWQNLNIALAWSETADVWRYPIETVS
                     LSEEGFERVYQGSCVFVHWPMWVKPNDEWNVSIVLHFSRDTRKFFQS"
     gene            complement(2565..3488)
                     /gene="ftsY"
                     /locus_tag="PK937_14710"
     CDS             complement(2565..3488)
                     /gene="ftsY"
                     /locus_tag="PK937_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNH33874.1"
                     /translation="MLSLLGKLKEKLTKTRQNLFGRISAVLQGKTILDEALLSEIEEI
                     LITADIGVQTAHKILERTRQRVKKEKISEAGQIYRLLEEEIENLIIPVAGKPLEEKMS
                     DKKPFVLLIVGVNGTGKTTTIGKLANRFAQAGKKVLMVAGDTFRAAASNQLEIWARRS
                     DVDIIRQNDGADPSSVIFDAMQSSATGKYDVVLIDTAGRLHTKINLMEELKKMRRVIE
                     KHIPGAPHETILVLDGTTGQNAIQQARQFSQAVQVSGLILTKLDGTAKGGVVLAIQQE
                     LKIPVLYIGVGEGIDDLEAFDPHAFIQAIVE"
     gene            complement(3490..3879)
                     /locus_tag="PK937_14715"
     CDS             complement(3490..3879)
                     /locus_tag="PK937_14715"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNH33875.1"
                     /translation="MDTKNPFDRKKFLPLIFWMAVIFILSSVPGKEYPSYDIPHLDKV
                     VHFGLYGVLGYAAWFAFAAYGQTKGKSASWMAGVAASVALIYAVSDELHQLYVPFRSC
                     DAADLIADAMGIGAVVGWKYFTFKDAR"
     gene            complement(3879..>6003)
                     /locus_tag="PK937_14720"
     CDS             complement(3879..>6003)
                     /locus_tag="PK937_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNH33876.1"
                     /translation="TVGEEEERQLGWFTRAVSWTGNNLIRILSLTFYDPEWGANRYFF
                     NAETFANDLKVINQLYNDKGYFDATIVKYTAKFDKHRESIEITIYIYEGEPTTLAEDP
                     DIHIQSPIPVADPGSKFQRKKILPLLTTRKGDPLDRINIESSKAAIQRLFSQNGYFAA
                     EVTELIDTASTEKRKARLEYTVTPGRYAVFGKTKMQGNYYKDYLPKGTTPDTTDKIVE
                     DYVPLRKVRYREGRPFNPDRLNLSMGQINGLGVFRSAKPIMNKVRGSLDSSTMVPSEK
                     LDSILHKSSNRITQKGAFLPDYGIPVDTLHITIAVAERKERSIKPGVGFTTDFSDLPE
                     GEAHWGNLPFLALQLSWQSRNFYGGARKMQVSAQVSKGFEQDKFFANFMETKISFRQP
                     TFKIPMWDDADNDLLTTLSAQRNNTVSYDAVTVEVTPTIIRQITAALNVNFTPFAFKA
                     VDTRRAATTNDINEAFTTNTRAGLTYNTANDFFYPTEGVSIFLTSDFAGFVLPSDLKF
                     IKINLDNRKYFGLTDRLSLAVRARGGSAIPYEGSKGSEIPVTEKFYGGGPNSVRSWGI
                     RELGIVAQNGGTLTYGGGNSIMEAGVEFRYLLYKARDPNEAILGMDLAAFADYGKVWE
                     RYDFKNNSYNGKRLPAMPVAAVGLGTRIRTLIGPLRVDVGYKLQDVSKLKVLNASGTA
                     VETITRANAKKISPIALQVTLGQAF"
BASE COUNT         1520 a         1527 c         1330 g         1626 t
ORIGIN      
        1 cgatcgtcgg aatgccattg atcacatcaa gctgtgagcc gatgccgtca aaatttccct
       61 gaaattcttc cttaacggtt ttcatttctt taggcgggat atagatcgaa tgcggatcaa
      121 gtcctttgag ggcaccgaca atagcatctt cgaccagttt atccgaattg acatcttcga
      181 cgtacgtctg tgtaacgatc gagagtacat attgtaattt ctgaatgctt ttgtaggcgt
      241 tttgtccggg cccggtaaac ccgaggatac ctatcgcggc gatacctacg ccgacggcaa
      301 tcaaccattt tttgcgcatg aaaaactccc gattaacttt gaaagaattt ccgcgtgtcg
      361 cgggaaaagt gcaatactat acttacgttc cattcatcat taggtttcac ccacatcggc
      421 caatgtacaa aaacacacga cccctgataa acccgttcaa atccctcttc ggatagcgaa
      481 accgtttcga tgggatatcg ccaaacatcg gccgtttcag accaagcgag tgcgatattc
      541 agattttgcc aaccgtctat catatttaaa ctccgaatac cggcaaatgt tcccgacgac
      601 tgcatcgcac cgtccggcca ttttctgccg ccgaccgcgt aaaaacgatc atccgcttgc
      661 ccggccaaaa gtgtcagatt gatctccgtt ccaaatttgg ctttaacctc ggttttgcct
      721 ctattgtgcc agcggtaatg tacttcaatg cgcggatcat cgctgaatat tttatatttt
      781 ttttcgattc gcatcggcgc catctctagt tccgtttcgc gcaggcatgt gatttccaca
      841 taacgttcgt attcttttcg ttccacatca tacgatgcat cggccagatc ggcatatacg
      901 ctgtaggtct gattttgcca tgcctccaaa tcacaattgt ccgtcaacac atgatctcta
      961 aacgaacggc gctcgtattg atcatagatc agatgcctat gtaaatccgg ttctttggat
     1021 aatacacgat catggatgct ggcaatgctg tcgtccgatt cggtattttt tttagatggc
     1081 tcggtgatgg aagcctccgc caactgatga tgatatcctt cttcccgccg ggtcaaaatg
     1141 tccgtcacgt gcatgggaat ctctttataa tcaatctcaa aaagcgtcgc accatgatgc
     1201 ggatcaaggt aggcatttag cttcggcgat tcaaaaagaa tttcattttt cccgttcgta
     1261 tcaaaatccg tttcttctaa acgataagcg gatccttggg tatatgcgtc cagctgatcg
     1321 gttagtactt cggcgttcag catatgccgg tacaccgcaa aacgaatatt attgaggtat
     1381 atccctccga atacaccgtg ccagtaaggg caattgcatt gcgaagccca caggtggttt
     1441 tgggcggatt ccagcagcga ttgttttacc ttatcgtctt ttggaatagc ttcagcaact
     1501 ttggccaacc gcgcactgac acgtaacatt tttttatgca tacggttgct ctcggcatac
     1561 ttactaaaaa aattacgcca aaaaccgcca cgcacaaacg cgttgaaaga ctcagcgatc
     1621 ttctcttctt tcagtttcga ctgaaacaat tcatagttac gataaccgct taacggcaat
     1681 gcccagtgca acatttcact atacgaagcc gtcggaaggt aaatttttcc tttgggcggg
     1741 accgatcgca ggacatcgga aaaatggcat acttcgatcc aatcccggtt cgcctcaagt
     1801 acttcaaaaa aacgttgaag ccacccattt tcatacacat gctgatatgt gtccggccat
     1861 atgccaaact tttcgccatc gtcggcaaat acggctacgg cgttctcatt tttttccgca
     1921 agatcacgca ggtaatctat cgttttttcc ggcgcttcga acgggatgag ataccgcaac
     1981 tgttgactga tcggaaacag cgccagcgtt gcgccctggt cttccgtgat gtagtatccg
     2041 ctcaaatcat cgaccgacaa gccggcgtga aggaaatgcg tatcatccag tatcacatac
     2101 cgcactccga cttgactgag caccgacggt aaatgcggtt cccacacacg ttcggccagc
     2161 cacatacctt gcgcggcttg ttcgtaccgt tgcaaaagat atttttgtaa gcgttgaatt
     2221 tgcccgacct gatcacgatg cgggataatc ggaagtatgg gctcataaaa tccgccgccg
     2281 ataagttcga tctgtccgcg atccgtcaaa gtgcgcagct gtatgctgta ttcgggatac
     2341 tgtttatcaa gccattcgta caaaataccc gacgtatgca tgcctacgcg aacaccggga
     2401 aaatcccgca gcatatcaag aaaaggccga taggcatttt gataagcctt atccaataca
     2461 aaatcaaaat tgccaacagg ctgatgattg tgaatgccca gtatgaggcg tatttttttc
     2521 attcgacgac agtttgacac acgtataaat gaacgctcaa caaattattc cacgatggct
     2581 tgaatgaatg catgcgggtc aaacgcttca agatcatcaa tgccttcgcc cacaccgata
     2641 tacagtacag ggattttcaa ttcctgctga atcgccagta ccacgccgcc tttggccgta
     2701 ccatccagtt tggtcagaat caatccggag acctgcacgg cttgcgaaaa ctgtcgcgcc
     2761 tgttgaatag cattttggcc cgttgtgccg tcaagcacca aaatcgtctc atgcggcgct
     2821 cccggaatat gcttttcgat cacgcgacgc atttttttaa gttcttccat cagattaatt
     2881 ttagtatgca gacgcccggc cgtgtcaatc agtacgacat cgtacttgcc ggtcgccgac
     2941 gattgcatgg cgtcaaaaat aaccgacgag gggtctgcgc cgtcgttttg gcgaatgata
     3001 tccacgtcgg agcggcgcgc ccagatttca agttgattac tcgctgccgc acgaaacgta
     3061 tcgccggcca ccatgagcac ttttttcccg gcctgtgcaa accgattggc caatttaccg
     3121 atggtcgtcg tcttgcctgt accgttgaca ccgacgatca aaagaacaaa cggttttttg
     3181 tcggacattt tttcttcgag cggtttaccg gctacgggga taatcaaatt ttcgatttct
     3241 tcttccaata atctgtaaat ttgaccggct tcgctgattt tttctttttt aacgcgttga
     3301 cgcgtacgtt ctaaaatttt atgtgccgtc tgaactccga tatcagccgt tatgagaatt
     3361 tcttcgattt ctgacagcaa ggcttcatct aaaatcgttt ttccctggag tacggcgctg
     3421 atgcggccaa aaagattttg gcgcgttttg gttaattttt ctttgagttt accgagtagt
     3481 gaaagcatgc taccttgcat ctttaaatgt aaaatatttc catccgacta ccgcaccgat
     3541 acccatagca tcggcgataa gatcggctgc atcgcatgat ctgaaaggaa cgtacagctg
     3601 atgcaattcg tccgaaaccg catagatcaa cgccactgac gcggcaaccc cggccatcca
     3661 tgaagccgat ttgcctttag tttgtccata cgccgcaaac gcaaaccacg cggcgtatcc
     3721 taaaacaccg tacaaaccaa agtgtacgac tttatccaag tgcggtatat catacgacgg
     3781 atattctttt ccgggaacag acgacaaaat aaaaatcacg gccatccaaa aaatcagcgg
     3841 caaaaatttt ttacgatcga aaggattttt cgtatccatc aaaacgcctg ccctaacgtc
     3901 acctgcaaag cgatgggaga gatttttttg gcattggcac gggttatagt ttccacggcg
     3961 gttcccgacg cattgaggac tttgagtttg ctgacgtcct gaagtttgta gcctacatcc
     4021 actcgcaacg gcccgatgag cgtgcgaatg cgcgtcccga ggccaacggc cgctacaggc
     4081 atagccggca atcgttttcc gttgtaggaa ttatttttga agtcgtagcg ctcccatact
     4141 ttaccgtagt cggcaaacgc ggccaggtcc attccgagaa tggcttcatt gggatcgcgg
     4201 gctttataga gtaagtagcg aaattctact cccgcctcca taatgctatt accaccgccg
     4261 tacgttagcg taccgccgtt ttgggcgacg atgcccagct cacggatacc ccagcttcgc
     4321 acactattgg gaccaccgcc gtagaatttt tccgttaccg gaatttcaga acctttactt
     4381 ccttcatacg ggatcgccga accgccgcgc gcgcgaaccg ctaacgaaag acggtccgtc
     4441 agcccgaaat acttacggtt gtcaagattg attttgatga acttaagatc ggatggtaaa
     4501 acaaatcccg caaaatcgct cgtcaaaaaa atcgaaacgc cttccgtcgg gtaaaagaaa
     4561 tcattagccg tattatatgt caaaccggcg cgggtattgg tggtaaaagc ctcattgata
     4621 tcattggtcg tagcggcacg ccgagtatcc actgctttaa acgcgaacgg cgtaaaattg
     4681 acattcagtg cggcagtaat ttgacgaata atcgtcggtg tgacttcgac ggtaaccgca
     4741 tcgtatgaaa cggtattatt acgttgcgcc gataacgtcg tcaacaaatc attatccgca
     4801 tcatcccaca tcggtatttt gaatgtcggc tggcgaaatg aaattttggt ttccataaaa
     4861 ttggcaaaga atttatcctg ttcaaaccct ttgctgacct gcgcggaaac ctgcatttta
     4921 cgagcgccgc cgtaaaaatt acgactctgc cacgaaagtt gtaatgcgag aaaaggcaga
     4981 ttgccccagt gcgcttcccc ttccggcaaa tcgctgaagt cggtcgtaaa acccacaccc
     5041 ggtttgatgc tgcgttcttt gcgttcggcc acggcgatgg taatgtggag cgtatcaacc
     5101 ggaatgccat agtcgggaag aaaagcgcct ttttgtgtga tacgattgga cgatttgtgc
     5161 aaaatcgagt cgagtttttc actgggcacc atcgttgagg aatcgagtga accgcgtact
     5221 ttattcataa tcggcttcgc cgatcgaaaa acgcccaaac cgttgatctg gcccatactc
     5281 agattgaggc gatccggatt gaacgggcgt ccttcacggt agcgcacttt acgcaatggt
     5341 acataatctt cgacgatttt gtcggtcgta tccggcgtcg tgcccttggg caggtaatct
     5401 ttgtaataat taccctgcat ctttgttttg ccgaacaccg cataacgtcc cggtgtcacc
     5461 gtgtattcca atcgtgcttt acgtttttcg gttgaagccg tgtcaattag ctccgttact
     5521 tcagcagcaa aatatccgtt ttgactaaag agacgctgaa tggccgcctt ggaagattcg
     5581 atattgatgc gatccagcgg atcaccttta cgggttgtga gcagcggtaa tattttttta
     5641 cgctgaaatt tggaacccgg atcggcgacg ggaatcgggc tttgaatatg aatatcagga
     5701 tcctcggcca acgttgtcgg ttcgccttcg taaatataaa tcgttatctc aatgctttcg
     5761 cgatgcttgt caaatttggc tgtatatttt acgatcgtcg catcaaaata acctttgtcg
     5821 ttgtacagtt gattgatgac ttttaagtca ttggcaaacg tctcggcatt aaaaaaatac
     5881 cgattggcac cccactccgg gtcataaaat gtcagcgaca aaatacgaat gaggttattt
     5941 cccgtccagc tcaccgcgcg cgtaaaccat ccgagctgac gttcttcctc ttcgccgacc
     6001 gta
//
LOCUS       DAOQGF010000163         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6608_length_5992_cov_6.492842, whole genome shotgun sequence.
ACCESSION   DAOQGF010000163 DAOQGF010000000
VERSION     DAOQGF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6608_length_5992_cov_6.492842"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="PK937_14725"
     CDS             complement(<1..1747)
                     /locus_tag="PK937_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNH33877.1"
                     /translation="MKNEKLTRKEIIDNRLKQAGWNVTDRTQVVEEFDIIVDKNLVQE
                     ASTPYAGHQYSDYVLLGKDGKPLAVVEAKKTSVDAAIGREQAKQYCYNIKETQGGELP
                     FCFYTNGHDIFFWDLDNYPPKKVHGFPTRDDLERYLYIRKARKSLAGELINTKIAGRD
                     YQIRSIRAVMEAVEKRKTKFLLVMATGTGKTRTCIALVDALMRAGWAERILFLVDRVA
                     LQNQTLDAFKEFLPNEPRYPKPYEKEIATDRRIYVSTYPSMLNIIRDEKNPLSPHFFD
                     LIVVDESHRSIYNTYQEILDYFNTITLGLTATPTDVIDHNTFQLFECEDGVPTFAYSY
                     EEAVNHIPPFLSNFQVMKIKTKFQNEGISKRTISLEDQQKLIVEGKEIEEINYEGTEI
                     EKKVINRGTNALIVREFMEESIKDTNGVLPGKTIFFCISKAHARRIEEIFDSLYPEYK
                     GELAKVIVSEDPRVHGKGGLLDQFTNNDMPRVGISVDMLDTGIDIREVTNLVFAKPVY
                     SYTKFWQMIGRGTRLLEQEKIKSWCTQKDNFLILDCWDNFEYFKLNPRGKELKGQIPL
                     PVRLFGVRLEKIEEAQ"
     gene            complement(2301..3203)
                     /locus_tag="PK937_14730"
     CDS             complement(2301..3203)
                     /locus_tag="PK937_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33878.1"
                     /translation="MKNYFKTILLFLSFVLTYALQIKAQEPDSASPLKPLVEAERNFA
                     KMAAAEGVRNAFVHNLADKALMFRNGPINGKELWEKRKPAPFLLKWEPEFADIAASGD
                     FGFTTGPWEIQEYRPLTESGGYGYFTSVWKKQTDGTWKVVLDIGTSNPKPAAYTYALQ
                     YPPGADKVRSFTPVAKLDAVRDELIQRDAALGRSTAASFSAPSFFEVLSSDARLQRNE
                     YLPTTHRDTIAARLRGITAWESQPIGGDVAASGDLGYTYGTYAIRRGETSEKGYYVRF
                     WKRNEKNQWKITLDLTDPSPEVKK"
     gene            complement(3223..3417)
                     /locus_tag="PK937_14735"
     CDS             complement(3223..3417)
                     /locus_tag="PK937_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33879.1"
                     /translation="MKWLTQSQKLYVLIFKISLFFVMPLLLLTVLPMIGVAWLSEIAL
                     AALVAGMILTITKLRERIFL"
     gene            complement(3624..4793)
                     /locus_tag="PK937_14740"
     CDS             complement(3624..4793)
                     /locus_tag="PK937_14740"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNH33880.1"
                     /translation="MKNAMYGLLWSWILVSVVHGQTIDKYKLDVLLKKAEQTHSEAVI
                     VYKDNQLVTEKYFGIGQADTKIESMSCTKSIVGLAVACMLSDQTLASLDEPVSRYYPE
                     WRQGQKQAITVRHLVNMTSGLQNHPNASVEIYPSPDFIQLALAAELTSKPGEVWSYNN
                     KSLNLMAGVIKKITGQRMDQYIGERLFKPLGITDFSWSLDSAGNPHVMSGCQIKPGDF
                     AKIGLLLLNNGKYNNQTVIAEKYIQEVITPCKPYPGYGILWWIDYEKTTSIVDDEIVR
                     ELQKAGVAEDFIQKAKAMQGTYANNDAYYAKLLSVFGANPWEYINTTLGANLRLRKKE
                     FSGHVTYRADGYLGNYIIVDFKTKIVAVRMISHQSFQDETDNFNDFAKMVLKLSE"
     gene            complement(4955..5413)
                     /locus_tag="PK937_14745"
     CDS             complement(4955..5413)
                     /locus_tag="PK937_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNH33881.1"
                     /translation="MRHNIKILIASVLLLACNSPEPKISVQSENEKIVRQYYEYFNNH
                     AWEKMAGMYTEVSEFKDPSLGPGIVKQTRQQIIQKYTELSTMFPDVKDEIIQTYPSGD
                     KHIIVEFVSTGTAPDNTKLQLPICTVFTIENGLISKDFTYYDNFETKTTN"
     gene            complement(5473..>5992)
                     /locus_tag="PK937_14750"
     CDS             complement(5473..>5992)
                     /locus_tag="PK937_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33882.1"
                     /translation="LHAARTDARTDATGHILLLAEQERRLWNQTMILKGLTHLHASAR
                     GMTITRYHLEAGIAGCHATAQSFAATNWDRIVQLYDLLLQTQDHPVIRLQRGIALCQS
                     QSPQTALAWLQQQGIDEALSDYPYLHATYAELYKALDDKPSARKALTRAIQCPISEAE
                     KHLLNDRLEKLA"
BASE COUNT         1503 a         1280 c         1401 g         1808 t
ORIGIN      
        1 tttgggcttc ttcaatcttt tccaatcgca caccaaacaa gcgaacaggc aaagggattt
       61 gacctttcag ttctttacct ctcggattga gtttgaaata ttcaaagttg tcccaacaat
      121 caagaatcag aaaattatct ttttgtgtgc accaagattt tattttttct tgttccaata
      181 atcgtgtgcc acgtccaatc atttgccaaa acttggtgta actgtaaact ggttttgcaa
      241 aaacaagatt ggttacttca cggatgtcaa tgcctgtgtc aagcatgtcg acgcttatac
      301 ctacacgtgg catgtcatta tttgtgaact ggtcaagtaa tccgcctttg ccgtgaactc
      361 ttgggtcttc acttacaata actttcgcta attcgccttt gtattcgggg taaagtgaat
      421 caaagatttc ctctattcgt cttgcatgtg ctttgctgat acaaaagaag attgtctttc
      481 cgggtaatac cccgtttgta tctttgattg attcctccat gaactcccga acaatcaaag
      541 cgtttgtgcc tcgattgata actttctttt caatctctgt tccctcgtag tttatttctt
      601 caatttcttt tccttcaaca attagtttct gttggtcttc caaagaaatt gttcgcttgc
      661 ttattccttc attctgaaac ttcgttttaa ttttcattac ctgaaaatta ctcaggaatg
      721 gcggaatgtg atttaccgct tcttcgtagg aatatgcaaa agttggaacg ccatcttcgc
      781 actcaaatag ctgaaaagtg ttgtggtcaa ttacatcagt tggtgttgct gtcaaaccca
      841 atgtaattgt gttgaaatag tctagaattt cttgataagt attgtaaata ctgcggtggc
      901 tttcatccac aacaatcaaa tcaaagaagt gaggacttaa aggatttttt tcatcacgaa
      961 taatatttaa catacttggg tatgttgaaa cataaattcg tctgtcggtt gcaatttctt
     1021 tctcgtatgg tttagggtat cgtggttcgt tgggtaggaa ttctttgaat gcatcaagtg
     1081 tttgattttg caaagcaact ctgtcaacta aaaataaaat acgttcagcc cagcctgctc
     1141 tcattagtgc atccaccaag gcaatacagg ttcgtgtttt acccgtgcca gttgccatta
     1201 ccagaaggaa ttttgttttc cgtttctcaa cggcttccat cacggcacga atggaacgga
     1261 tttgataatc cctccctgca atttttgtat tgatgagttc accagctaaa ctctttcgtg
     1321 ccttgcgtat gtataggtag cgttcaaggt cgtctcttgt cgggaagcca tgaactttct
     1381 ttggcggata gttatctaaa tcccaaaaga aaatatcgtg cccgttggtg taaaaacaga
     1441 agggaagttc tccaccctgt gtttccttaa tattgtaaca atattgtttc gcttgttctc
     1501 ttccaattgc agcgtctacc gaagtctttt tggcttctac aactgcaagc ggttttccgt
     1561 ctttgcctaa taatacatag tcgctatatt ggtgccctgc atatggtgtc gaggcctctt
     1621 gaacaaggtt cttgtcaaca atgatgtcaa actcctcaac tacctgagtt cggtcagtca
     1681 cattccatcc cgcttgtttc aaacggttgt caatgatttc tttccttgtc agtttttcgt
     1741 ttttcactta aaaatgaaaa ttaataggtt taattctaac gcagtgattt atattcgttg
     1801 tacattgcca tgcgaaaaac attaaatctt ttttggagtt ttagagatta atataaagat
     1861 gcgcctatca caggttttat gcaagagtta atacgtacaa ggctgtgcgg gaatatcgca
     1921 ttcacggcat acgatggatg tgttgaaaaa aaatgattct cgccgagacg ttactaatcg
     1981 atggatacga gcaggcgtta cacacggaag tgcatgatga ttttaactat tatacagaat
     2041 caattctttc ggatgtgact gagcgaggat tgggtttcct ggtcatttcg ataaggcaag
     2101 tctacacagt aaccgggata gaatacgttc tcgcagagac gccaaggtac aagaggttta
     2161 ttaagaatat ttttcaggtt ctcctagcga ctttgcgagg gattggtttc ctggtcattt
     2221 cggtacagca aacctacgca atgaccggga tgtgggatac gttctcgcga agacgccaag
     2281 ataaaaaatc agcgacagtt ttattttttg acttccggtg aaggatcggt gagatccagt
     2341 gtgattttcc attggttttt ttcgttacgt ttccagaagc gcacgtagta gcccttttcg
     2401 gaagtctcac cgcgccggat ggcgtacgtc ccgtacgtgt agccgaggtc gccggaagcg
     2461 gctacatcgc cgccgatggg ttgggattcc caagcggtga tcccgcgcaa acgtgctgcg
     2521 atcgtatcgc gatgggtcgt gggcaggtat tcattgcgct gcaaacgtgc atcgcttgat
     2581 agcacttcga aaaacgacgg ggccgagaat gacgctgccg tcgagcggcc cagcgcggcg
     2641 tcgcgctgga tcagttcgtc ccgaacggca tcaagtttag cgacgggggt aaacgatcgg
     2701 actttatcgg cgcccggcgg gtactgcaaa gcgtaggtgt atgctgccgg tttcggattg
     2761 gatgtgccga tgtcgagtac gactttccat gtgccgtcgg tttgtttttt ccataccgat
     2821 gtgaagtaac cgtagccgcc ggactccgtg agcgggcgat attcctgtat ctcccaagga
     2881 cctgtcgtaa aaccaaaatc accggaggcg gcgatgtcgg caaactccgg ttcccatttg
     2941 agtaaaaacg gcgcgggttt gcgcttttcc cataactctt tgccgttgat agggccgttg
     3001 cgaaacatca atgctttatc ggcaagattg tgaacgaagg cattgcgcac gccttcggcg
     3061 gcggccattt tggcaaaatt gcgctcggct tcgacaaggg gtttgagcgg cgaggctgag
     3121 tcgggttcct gcgctttgat ttgtaacgca taggtcaaaa caaaactcaa aaaaagaagg
     3181 atggttttga aataattttt catgggacat acggggttag atttataaaa aaatacgttc
     3241 gcgcagtttg gtgatcgtca ggatcatacc ggcgaccaaa gcggctaagg ctatttcgga
     3301 taaccaggca acaccgatca tcggcagtac cgttagtaaa agcaaaggca tgacgaaaaa
     3361 taaactaatt ttgaaaatga gtacatacaa tttttgagat tgtgttaacc acttcacgat
     3421 agagcctttc ctgaaacttt gtagaacaga ctacggatgc gatcgtaata gagatatacg
     3481 catacagagg aaaaagtttc agaaaaaccg ggtttagagg cccttctgtc tgagcgaagt
     3541 cgaagacgga ttttaccaaa cttggtcatg gttcgactcc gcttaccatg acgtaaatga
     3601 gtcattccga taagcgcatt attttattcg gatagtttta ataccatttt tgcaaaatca
     3661 ttgaagttat cggtttcgtc ctgaaaactt tggtggctga tcatacgcac ggcgacgatt
     3721 ttggttttaa aatccacgat gatatagttt cctaaatacc cgtccgcccg ataggtcaca
     3781 tggccgctaa attctttttt gcgcagtcga agattggcgc cgagcgtcgt attgatgtat
     3841 tcccatggat tagctccgaa aacgctgagt agtttggcat agtaggcatc gttatttgcg
     3901 tacgtgcctt gcatggcctt ggctttttgg ataaagtctt ctgcaacgcc tgctttctga
     3961 agttcacgca cgatctcatc atccacgatg gaggtcgttt tttcgtagtc aatccaccac
     4021 agtatcccgt aacccggata cggtttgcac ggtgtgatca cttcttgtat gtatttttca
     4081 gcgatgacgg tttggttatt gtattttccg ttattgagca agagaagtcc gattttagcg
     4141 aagtcgccgg gtttgatttg gcacccggac atgacgtgcg ggttaccggc actgtccagc
     4201 gaccaactga aatccgtgat acccaagggt ttgaaaaggc gttcgccaat atactgatcc
     4261 atgcgttggc cggtgatttt tttgatcaca ccggccataa gatttagtga tttgttatta
     4321 taggaccaga cttcgccggg tttggatgtt aattccgcgg ccagcgcaag ctgtatgaag
     4381 tcggggctcg gatagatttc aacggaagca ttgggatgat tttgcaaacc ggatgtcata
     4441 ttgactaaat gccgcactgt gatcgcttgt ttttggcctt gccgccattc cggataatat
     4501 cgggacacgg gttcatccag actagccagc gtttgatcgg agagcataca cgccacggcc
     4561 aaaccgacaa tacttttggt gcaggacatg ctttcgattt tggtatcggc ctgtccgatt
     4621 ccgaaatatt tttcggtaac gagttggttg tccttataga cgatcacggc ttccgaatgc
     4681 gtctgttcgg ctttttttaa aagcacgtcc agtttgtatt tatcaatagt ttgcccgtgc
     4741 acaacggaaa ccagaatcca actccataac agcccgtaca tcgcattttt cataattcat
     4801 ttcgttttag aatgccatca acacgtcgga attacgtgtt acaaagtgtt accacctcaa
     4861 acgtaaatcc gtaaacaaaa gtttcatcgg catgcatgat gcgtgaagcg cagggctgcg
     4921 tcgtatgccg gattagtact aagaaccaga aaaatcagtt cgtcgttttt gtttcaaaat
     4981 tatcgtaata cgtgaagtct ttgctgatca agccattctc aatcgtaaaa acggtacaaa
     5041 tcggtaattg aagcttggtg ttatcgggcg ccgtcccggt agacacaaac tcaacgatga
     5101 tatgtttatc accggaaggg taggtctgaa tgatttcgtc tttgacgtcg gggaacatcg
     5161 tactcaattc ggtgtatttt tgaatgattt gttgccttgt ttgttttacg ataccgggtc
     5221 ccagcgaagg atctttaaat tccgatacct ccgtgtacat gcctgccatt ttttcccatg
     5281 cgtgattatt aaagtattca taatactgcc ggacgatttt ttcattctcc gattgtaccg
     5341 aaattttcgg ctcggggctg ttgcatgcta aaagcaaaac tgatgcgatt aaaattttga
     5401 tgttatgtcg catagatatt tttctttcga aatgatatgg tggattgatc gagagcgctt
     5461 actaaaatta aactaagcta atttttctaa acgatcgttt agcaaatgtt tttccgcttc
     5521 gctgatcggg cattggatag cgcgggttaa ggctttgcgg gcggagggtt tgtcatcaag
     5581 ggctttgtat aactcggcgt acgtggcatg caaatacgga taatcgctca gtgcctcgtc
     5641 aatgccttgc tgctgaagcc aggcgagcgc cgtttggggc gattgggatt ggcaaagtgc
     5701 gatgcctcgc tggagtcgga tcacgggatg atcctgcgtt tgcaaaagca gatcatacaa
     5761 ctgcacgata cgatcccaat tggtcgcggc aaaactttgt gccgtggcat ggcaaccggc
     5821 gataccggct tccagatgat accgtgtgat cgtcatgccg cgtgccgagg cgtgcagatg
     5881 cgtaaggcct ttgagaatca ttgtttgatt ccacaaacgg cgttcttgtt cggccaacaa
     5941 aagaatatgg ccggtggcgt cggtacgcgc atcggtacgt gcggcgtgca gg
//
LOCUS       DAOQGF010000164         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6646_length_5974_cov_6.438925, whole genome shotgun sequence.
ACCESSION   DAOQGF010000164 DAOQGF010000000
VERSION     DAOQGF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_5974_cov_6.438925"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="PK937_14755"
     CDS             complement(<1..1774)
                     /locus_tag="PK937_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33883.1"
                     /translation="MTSPDPMFAHLDHVLRNTSTHGTQDHTRAEQKSEKAAKPFIPES
                     KAADLSQTSLFWKNLRAFYRGDASGAIAKNRLPALLLPFRDTDRIRFDYPVWIDVSSD
                     DEKPVRTLNQLLEDAIVSFAPGADDAKILKDNRLRLEQMMRQYLSENGGSAACASIWE
                     TAVTRLTEQLHLKGETEKSFLSDTKRLKDHLPKDGHLYDFSKAMALTLLSSALRHRVA
                     DARTRLLKDAAVLRQRLQEMILIEDHRNPEAKNSSAAPGNHGYGSAFVDFTAVSRVLP
                     ASPTLPMAPARRKRITEALNILSQPDNIFKESALLIVTPDIEARYGMAWTDTMTSARI
                     SKAPSAEALRLAEAQFDAYMQTITSWVRAKRIAQLESQDRYEADVHDVYFEKFDWRAL
                     SDEEVALCAPVVVLASDEEILRDHLHDFSTALASRRPIKFFVMQSRYHSDQAAPTGVI
                     SFAHDLAAMAVAHRQAYVYQAAMVHPEQVFHGIRTGLSQSAPSLFYMLAPVSEHALLA
                     SGSALESRAFPEYSYDPQRGVQWGSRFSVEANPQADSDWPVYELTATQYDKTETLLRL
                     PFTFADHLVFDDTMTHHFMDVPPSH"
     gene            complement(1802..2755)
                     /locus_tag="PK937_14760"
     CDS             complement(1802..2755)
                     /locus_tag="PK937_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNH33884.1"
                     /translation="MFGLKSDCVLDIPEDYYTLADYESATARWCPGCGDHGILTAVQR
                     LCSEKQLPPEKTVFVSGIGCSSRLPHYMKTYGFHGLHGRALPVANGIKFRRPDLHVFV
                     ATGDGDCCSIGTAHWIHAIRYNMNITVLLLDNEIYGLTKGQASPTSNKGTVSNTSPRG
                     SQLPPINPIQTTLGVSNVSFVAQTVDWNPAHLYATIEAGYNHPGLSFIRVLQRCPVFQ
                     KDLFQTTMSSPDAITLLTHKNGIQVDPAITKIYKNVVEHDPSDINQARTLAGNHDREV
                     IGLLYQNKNAERYDDYGAHNLGISPEDKMRAIHKELDKFAV"
     gene            complement(2799..4547)
                     /locus_tag="PK937_14765"
     CDS             complement(2799..4547)
                     /locus_tag="PK937_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNH33885.1"
                     /translation="MDRRDHSCRNPPARSKAGASGIRIRIGSQKITNMGDEADLVVAL
                     NEQVLYGRIDQKAFRPGTKILLDNKWAKHDVKKIQDEYTEGLNDLKSRGYDVIEIPIE
                     QESMKHIADPRKGKNMWVFGMLCCIYQRDTAKAENQIRHTFRKKKQDVVDVNLKLFRA
                     GYAWAEQNLKFQYDIPAMATDEQFVVMNGNEAISLGTLAAGIELCSMYPITPATSASH
                     HLAECFEAAGGLIHQAEDEIAAIGFAIGASYAGKTAITITSGPGMALKTEFIGLAVMA
                     EVPLVIVNVQRGGPSTGLPTKVEQGDLLYALYGQPGDAPKIVLAAATIEECFHFVILA
                     RRLAEAFRMPVILLSDANLATGVQPYPRPQINQEWFAPPIDQSPWREGVPAFDWDEET
                     GLSRRPIPGQKNGMYTLTGLAHDQRSKVAYEAATNQVGCDMRSRKLATFLQSLKPPVV
                     NGDPEGDLLIVGWGSTLGAIEEAVNHARELGGRVSSLHLRFLSPIEPGLKDIFKRFKK
                     VMTVEINYSDNLNDPLITPENRRMSQLAWYLRAHTLTDVDCFSNVHGQPIRPGMILDM
                     IKKELSLVENDEPVMA"
     gene            complement(4454..4702)
                     /locus_tag="PK937_14770"
     CDS             complement(4454..4702)
                     /locus_tag="PK937_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33886.1"
                     /translation="MKESTSSGGNHASVQKTLVHEHIVEIVSDSGEGAQKAGQSLGML
                     SAKMGNGVWTVEIIPAEIRRHVPKPVRRVFVSALAPKK"
     gene            5142..>5974
                     /gene="cax"
                     /locus_tag="PK937_14775"
     CDS             5142..>5974
                     /gene="cax"
                     /locus_tag="PK937_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HNH33887.1"
                     /translation="MNVGKFKIEPLMLLLVFVPISIVLEVMHADPVWIFVSACGAIIP
                     LAGLMGKATEHLSHNLGAGLGGLLNATFGNAAELIIALFALKAGLHDVVKASLTGSII
                     GNILLVLGLSITVGGLKFDKQTFNRTASSNGATLLALSAIALVVPAIFHMLVKGHPDA
                     HEQELSLEIAIVLFVIYILSLIFSLRTHKHLYAGDSAEVPHEHDGPIWSKNKAIGVLL
                     AATAFVALMSEFLVGAVEHTAKTFGMTDVFVGVILVAIIGNAAEHSTAILVSAKNQMD
                     LA"
BASE COUNT         1422 a         1412 c         1560 g         1580 t
ORIGIN      
        1 agtgcgaagg cggcacatcc atgaagtgat gcgtcatcgt gtcatcgaac acaagatgat
       61 cggcaaacgt gaaaggcaat cgcagcaagg tttcggtttt atcatattgc gtggcggtca
      121 attcatacac cggccagtcg ctgtcagctt ggggattggc ttccacacta aaccggctgc
      181 cccattgaac gccgcgttgc ggatcgtagg aatattcggg gaaagcgcgg ctttctaatg
      241 cggaacccga tgccagcagt gcgtgttccg ataccggtgc caacatataa aacaacgatg
      301 gtgccgattg agacaatccg gtgcggatac cgtgaaacac ttgctcggga tgaaccatcg
      361 cggcctgata cacatacgcc tggcgatgtg cgaccgccat cgcggccaga tcatgagcaa
      421 atgagataac acctgtcggc gccgcttggt cactgtggta tcgtgattgc atgacaaaaa
      481 atttgatcgg acgccgcgaa gctaaggctg tcgaaaaatc atgaagatgg tcacgcagga
      541 tttcttcatc cgaagctaaa acgacgaccg gcgcacacaa agccacctcc tcatcgctca
      601 atgcacgcca gtcaaatttt tcaaaataca catcgtgtac gtcggcctca taacgatctt
      661 gcgattcaag ttgcgcgatg cgtttggcac gaacccacga ggttatcgtt tgcatgtacg
      721 catcaaactg cgcttcggcc aatcgcagcg cctcagcact gggcgctttg gatatccgtg
      781 cggaggtcat agtgtcggtc catgccatac cgtaacgcgc ttcgatgtcc ggcgtgacga
      841 taagtaatgc cgattctttg aaaatattat cgggctggct gaggatattc aatgcttcgg
      901 ttatccgttt gcgtcgtgcc ggcgccatcg gcaaggtcgg cgatgccggc aaaacgcgtg
      961 ataccgcggt aaaatcaaca aacgcactgc cgtaaccatg attgccgggt gcggctgatg
     1021 aattttttgc ttcaggattt ctgtggtctt cgatgagaat catttcttgc aaccgctgac
     1081 gcaaaacagc ggcgtctttg agaagccggg tacgtgcatc cgcgacacga tgacggagtg
     1141 ccgatgaaag caatgtaagc gccatggctt tcgaaaaatc ataaaggtgc ccgtccttgg
     1201 gcaaatgatc tttcagacgc ttcgtgtccg ataaaaaaga tttttctgtt tcgcctttga
     1261 gatgcaattg ttcggtaagc cgcgtgactg ctgtttccca gatcgaagca catgcggcgg
     1321 agccgccgtt ttcactcaga tattggcgca tcatttgttc taaccgcagc cggttgtctt
     1381 tgagtatttt ggcatcgtcc gcgcccggtg caaaagatac tatcgcgtct tccaaaagtt
     1441 gattgagcgt gcgaacgggt ttctcgtcat ccgatgaaac gtcaatccaa acgggataat
     1501 caaaccgaat gcgatcggta tcgcggaatg gtaagagcaa tgccggcaac cggtttttgg
     1561 cgatcgcgcc ggaggcgtca ccgcgataaa acgcacggag atttttccag aacagtgacg
     1621 tctgcgaaag gtctgccgct ttggattccg ggataaatgg tttcgccgct ttttcggatt
     1681 tttgttccgc acgggtatgg tcttgcgtac cgtgcgtcga cgtgtttcgc aacacgtgat
     1741 cgagatgggc aaacatagga tcagggctcg tcatgggagc ttcctcattt ttcataaaat
     1801 ttcaaaccgc aaatttgtct aattccttgt ggatcgcgcg cattttgtcc tccggtgaaa
     1861 tgccgagatt gtgcgcaccg taatcatcat accgttcggc gttcttattc tgatacaaaa
     1921 ggccgatcac ttcgcggtca tgattgcccg ccaaagttct ggcctggttg atatccgacg
     1981 gatcatgctc gaccacgttt ttatatattt ttgtgatcgc ggggtcaact tgaattccgt
     2041 ttttatgcgt caaaagtgtg atcgcatcgg ggctgctcat ggttgtttga aaaagatctt
     2101 tttggaaaac cgggcaacgt tgtaaaacgc ggataaacga caaaccggga tgattgtaac
     2161 cggcttcgat cgtagcgtac agatgcgccg gattccaatc taccgtctgt gcgacgaagg
     2221 aaacattgga gacgccgagc gtggtttgta tcggattgat cggaggtaat tgcgatccgc
     2281 gcggtgaggt attggaaact gtacctttat tggaagtcgg tgaagcttgt cctttggtca
     2341 atccataaat ttcgttgtcc agaagcagta cggtgatatt catattatag cgaatggcgt
     2401 ggatccaatg cgccgtacca atagagcaac aatcgccgtc gccggtggcg acaaacacat
     2461 gcagatccgg acggcgaaat ttgataccat tggctacggg aagtgcacgg ccgtgcaaac
     2521 cgtgaaatcc gtaggttttc atataatgcg gcagacggct cgaacagccg atgccggaca
     2581 caaatactgt tttttcgggc ggtaattgtt tttcgctgca cagtctttgt acagcggtca
     2641 aaatgccgtg atcgccgcat cccggacacc agcgtgccgt tgcgctttca tagtcggcta
     2701 acgtgtaata atcctcaggt atatcgagga cgcaatcgga ttttaatcca aacacgggag
     2761 tgctccttta tatgatttta ttattatagt tgacatggtc aggccataac cggttcgtcg
     2821 ttttcgacca gggataattc ttttttgatc atatccaaga tcatgcccgg gcgtatcggt
     2881 tggccgtgca cattggagaa acaatccaca tcggtcaatg tgtgagcgcg caagtaccag
     2941 gccagttgcg acatccggcg gttttccggc gtgatcagcg gatcattcag attatccgaa
     3001 tagttgatct ccaccgtcat gacttttttg aatcgtttga aaatatcttt gagacccggt
     3061 tctatcggcg agaggaaacg cagatgcaga gacgatacgc gaccgcccaa ttcacgggca
     3121 tgattgacgg cttcttcgat cgcaccgagg gtcgaacccc agccgacgat caggagatca
     3181 ccttcgggat cgccgttgac gaccggcggt ttgagtgatt ggagaaatgt ggccagctta
     3241 cggctgcgca tatcgcaacc gacctgattg gttgccgctt cgtacgcgac tttgctgcgc
     3301 tggtcatgag ccaatccggt gagagtgtac atcccgtttt tctggccggg tatcgggcgg
     3361 cggctgaggc cggtttcttc atcccaatca aacgccggta cgccttcgcg ccacgggctt
     3421 tgatcaatag gcggtgcaaa ccactcctga ttgatttgcg gacgcgggta tggctgtacg
     3481 ccggttgcga gattggcatc gctgagcaaa atgaccggca tgcggaaagc ctccgcaaga
     3541 cgccgggcga gaatgacgaa gtgaaaacat tcttcgatcg tcgcggcggc taaaactatt
     3601 tttggggcat cgccgggttg gccgtatagc gcatagagta aatcaccttg ctcgactttg
     3661 gtcggtaaac cggtcgaagg tccgccgcgc tggacattga cgatcacaag cggaacttcc
     3721 gccatgaccg caaggccgat gaattcggtt ttgagcgcca tgccgggtcc ggaggtgatc
     3781 gtgatggccg tttttccggc atacgatgcg ccgatggcaa aaccgattgc ggcgatttca
     3841 tcttccgctt gatggatcag cccgcccgcc gcttcaaaac attcagccaa atgatgcgat
     3901 gccgatgtcg ccggcgtgat gggatacatg gaacacaatt cgatgcctgc ggccaatgtg
     3961 ccgagactga tcgcttcatt accattcatc acgacaaatt gttcatcggt cgccatcgcc
     4021 ggtatgtcat attgaaattt gagattttgt tcggcccagg cgtatcccgc acggaacagt
     4081 ttgaggttga catccacgac gtcttgcttt tttttgcgga aagtatgacg aatctgattt
     4141 tcggctttgg ccgtatcgcg ctgatagatg cagcacaaca tgccgaaaac ccacatgttt
     4201 ttgcccttgc gcggatcggc gatatgcttc atggattctt gttcgatcgg tatctcgatc
     4261 acgtcatatc cgcgcgattt cagatcgttg agaccttcgg tatattcgtc ctgtattttt
     4321 ttgacatcat gtttggccca cttgttatcc agaaggattt ttgtacccgg gcgaaacgct
     4381 ttttgatcaa tgcgcccata aagcacctgt tcattcaatg caacgaccag gtcggcttcg
     4441 tcgcccatat tggttatttt ttgggagcca atgcggatac gaatacccga cgcaccggct
     4501 ttggaacgtg ccggcggatt tcggcaggaa tgatctcgac ggtccaaact ccattgccca
     4561 ttttggcgga caacataccg agcgattgac cggccttttg tgcaccttca ccagaatcgc
     4621 tgacaatttc tacaatatgt tcgtgaacta aggttttttg gactgaggca tgatttcccc
     4681 cggaagaagt ggattctttc atgatttcct cgttgatatc ggttaataaa ggacttaaaa
     4741 agactatatc attacttttg tcaggataaa tgtcgtttcg gaattctcat gaaacaatga
     4801 cattagtctt gaatcggtat gacctttata aggcggcgta cagtcggtag ggagaaaaat
     4861 cacgataaaa caaaaaatta gattgtgagg aaaccgtcta taagaataag taaagtacag
     4921 aaatatttat gatgtcgagg ccgcaaaatg aactcggata ttatattaac gtagccattt
     4981 cttagaaatc gatggaagta atgtatatcc aaaaatcatg tgttctttgg cgggtcttgc
     5041 ttttgtctcg tttatttgat taaataaacg cacctgcctg tgcgattact ttatatagtt
     5101 gaaaaaatac gtccgtaaaa taataaaagg agccccatcc aatgaacgtc ggaaaattta
     5161 aaatcgaacc gttgatgcta ttgttggttt ttgtgccgat cagtattgta ctagaagtga
     5221 tgcatgccga tccggtttgg atttttgtgt cggcgtgcgg agcgattatt cctttggcgg
     5281 gccttatggg caaagccacg gagcatcttt cgcataatct cggcgccggg ttgggtggct
     5341 tgctcaacgc gacgtttggc aatgcggcgg aattgatcat cgcgcttttt gcgctcaaag
     5401 ccggtttgca cgatgtagtc aaagcgtcgc tgacgggatc cattattggg aatattttgc
     5461 ttgtgctcgg tctgagtatt actgtgggcg ggcttaaatt tgataaacaa acgtttaatc
     5521 gtaccgcttc cagtaacggc gctacgcttt tggccctgag tgcaattgcg ctggtagtac
     5581 cggccatttt tcatatgctc gtcaaaggtc atcccgatgc gcatgaacag gaactgagtc
     5641 tggaaatcgc cattgtactt tttgtgattt atattttgag tttgattttt tcactgcgca
     5701 cgcacaaaca tttgtacgcc ggtgacagcg ctgaggtgcc gcatgaacat gacggtccta
     5761 tttggtcaaa aaacaaagcg atcggcgttc tcctggccgc aacggcattc gtagccctga
     5821 tgagtgaatt ccttgtgggc gccgtcgaac atacggctaa aaccttcggc atgacggacg
     5881 tttttgtcgg tgtgatttta gtggcgatta tcggtaatgc ggcagaacac agtacggcta
     5941 ttttagtgtc ggctaaaaat caaatggatc ttgc
//
LOCUS       DAOQGF010000165         5962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6670_length_5962_cov_7.208566, whole genome shotgun sequence.
ACCESSION   DAOQGF010000165 DAOQGF010000000
VERSION     DAOQGF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6670_length_5962_cov_7.208566"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5962)
                     /locus_tag="PK937_14780"
     CDS             complement(<1..>5962)
                     /locus_tag="PK937_14780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNH33888.1"
                     /translation="AFSNEDAVVPVIEAGNALVLDGVDDYILADSVDLVSTDFTIEFW
                     VKRNAVGNLIISQGALPASTNNFLDIGFEVTSNIYMEFWNNRIQSSYTFDNNWHHIAF
                     TANRSTGERVLYIDGKYETSNFSGIYTGQGKLYIGAESMTLGGPFNGSLDELRIWNHV
                     RSQSQISDQMTKSLRGDEVGLMNLWHFDESDPGTIVYEGSTSANDGVLNNGQGNNFTP
                     SGAMAPVAPAGVYAIAHNGQVELKWNKNTDADFFRYHVYSGITANPTTDIGTTTDGDV
                     NDTTFIATGLVNGQMYYFRVAGEDSAGQIGNYSTQDAAVSVVESGNALALEAGNSEYV
                     ALPSTFENITMTISAWIKPSSAQQHAIWSNRGSSGTNEDGFALGLLPDGTLYYEYGFP
                     SGAVSTSSGQIPINSWTHVALTLDGSIARLYVNGVEQANGAYSRTTSGVNAWIGGYNN
                     GWGTFDGQIDELSIWNYALAPAQITSGLAKPYRGDEVGLVALYHLDEPSASTTALDAS
                     PNAYNGNLVNGADFVTSTAMAPTAVAGISATTFFPDSVRITWNHNSETDLKKYIIYRS
                     TTPGFIPSLTDSIDQTAVGDTAFVDVNAVVGNIYYYRVSAVDSADQAGVYSSDVSADF
                     DPLLVKNTADAGEGSLRAAIAAAASLPGADSIRFAISNATIFIDSDLDINDASGPVVI
                     DGETNNNTVQVSVSFPFGDNTIFSVQSPNVTIRNMTLDAQNGSAQARFGIFFDDAGLA
                     GDNSKAENLTVRNAQRYGIIGQTSNDSVINSTIYNNAWGGVGSTGANWVIRNNRIGTD
                     ATGTIGLGNQDLGVYIFAMATVDSNIIAGNDSTGIYAAGPLNTIRGNRIGIGSDNSTI
                     PNNYHGIALNAGGGSQIIGNVISNHPQSGILIAGSSYNKVQDNLIGTDASGIIGYPNL
                     IGISLIGGSKYNEIGDTLGVNQNVISGNTNTGILITDAGTDSNYVWGNLIGTDINGTS
                     DLGNGSWGIALDNNSMHTAIGGMTSNHRNVISGNGNSGILMQGFSAHNVVQGNYIGTD
                     INGNVDLGNTQSGVFILNQAHDNIVGGTSPGAGNVISGNNNSGITIGNIGTRGQIIQG
                     NLIGTNATGNAAISNGSGVVLNSGHANLVGGTVAGARNVISGNTNSGIEITNVGSDTN
                     LVQGNFIGTDVNGTVPLGNGIGVTISEGGSGSPRLNLIGGATAAERNIISASTNYGVW
                     FINSSENKVQGNYIGTDVTGTQNLGNSSDAINLSNGASRDSIMGNTIAFSNTNAIAML
                     NSGTDSIIVHDNVIFGNGNGFYLQAGTQSDITPPQIISVGADSTVYGIASPNAFIQVY
                     ADSINQGRFKLGTTTANASGDWSLKVNIPAGTAITAMQDSAQGTSAFSMPFAPQPEIV
                     VYTLTGTYLDYGGIFSGNGQIDTVFVKNIGAGALDITSLSIENGSSQFTIVDTLPNNL
                     TLTPGDSVKLVVRFLPDGFSSYADSVTIISNDADESITHVAVYGTGVSGILGSFVVNP
                     DFGNVPVGDSSSVTEVKLFAVSGNVLISGMPLMLAGGSFDIDSVSGPAFLGLTDTMRV
                     FVKYHANVFGIERDTISVNHNGQNSSPLMVALLGTGISGSLAALPSNYDFGNVAVGDS
                     VDTVIKLASNGGAVAVSGSALNGGGYSIVSSIGPSTLYSGDTLVYTIRYKPTTFTTTL
                     DTLKITNNSVTSTYTVALSGTGVSGTLVALPSSYDFGNVAVGDSMDAVIKLASNGGAV
                     AVSGSALNGGGYTIVSESGPNVLFSGDTLVYTIRYKPTTFTTTLDTLKLTNNSSASTY
                     TVAFSGTGASGTLLASSVALNFGNVQVGQSAQQTIKFYSQGGAVTVSSTLAGPAFQVV
                     SATGPSTLYAGDTLSVTLQYNAGVFGIETDTLRVSTNATNGGMNIALDGIGVPGTLVA
                     LPSSYDFGNVAVGDSAQANIKLYATGGAVIVHGVTNMSAYFEIGSMNLPDTLYAIAGD
                     TLTIPVKYLADAFGTRADTLAVNSNSGLL"
BASE COUNT         1644 a         1549 c         1256 g         1513 t
ORIGIN      
        1 tcaaaagacc gctgttgctg ttcacagcca gtgtgtcggc acgtgtgcca aacgcatccg
       61 ctaaatactt caccggtatc gtgagcgtat ctccggctat cgcatagagc gtatccggca
      121 gattcatact accgatttca aaatacgcac tcatattcgt cacgccatgc acgatcaccg
      181 caccgcctgt agcataaagc ttgatgttcg cttgcgcgct atcacctaca gcaacattac
      241 caaaatcata acttgacggt aacgccacga gtgtacccgg cacaccaata ccatccagtg
      301 ctatattcat accgccgtta gtcgcattgg tgcttacacg cagcgtatcc gtttcgatgc
      361 cgaagacacc tgcattatac tgtagcgtta cactcagtgt atcgcccgca tataaggtcg
      421 aaggccctgt cgccgatact acctggaatg ccgggccggc cagcgtactg cttaccgtta
      481 cggcgccacc ctgactgtaa aacttaatcg tctgctgtgc actctgacca acctgcacat
      541 taccaaaatt cagcgctacg ctacttgcca gcagcgtgcc tgatgcacct gtacctgaaa
      601 atgctactgt atatgttgat gcggacgagt tattcgtaag cttcagtgtg tccagagttg
      661 tcgtaaacgt ggtcggttta taacgtatcg tatacaccaa cgtatcaccg ctgaaaagaa
      721 cattcggacc actctccgaa acaatcgtat aaccgccgcc attgagtgcg ctgccgctta
      781 ctgcgaccgc accaccattg ctggccagtt tgatcaccgc atccatacta tcacctacag
      841 ccacattgcc aaaatcataa cttgaaggca atgctaccag tgttcccgaa acgcctgtac
      901 ctgagagcgc gaccgtatat gtcgaagtta ccgagttatt tgtaatcttc agcgtatcaa
      961 gagtcgtcgt aaacgtcgtc ggtttatagc gtatcgtata taccagcgta tcacctgaat
     1021 acaatgttga tggaccgatc gaagatacga tcgaataacc gccgccattg agtgcacttc
     1081 cactcactgc aactgcacca ccattgctgg ccagcttgat caccgtatcc acgctatcgc
     1141 ctacggctac attgccaaaa tcataattgg acggcaaagc agctaaagat ccagagatac
     1201 ctgtgcctag aagcgctacc atcaacggag atgaattctg gccattgtga ttgaccgata
     1261 tcgtatcgcg ttcaattcca aacacattag catgatattt tacaaacaca cgcatagtgt
     1321 cagtcaaacc gaggaacgca ggaccgctaa cggagtcaat atcaaacgaa ccaccggcca
     1381 acatcaatgg cataccgctg atcagcacgt taccgctcac ggcaaacaat ttaacttccg
     1441 ttacggatga agagtcaccg acaggaacat taccaaagtc gggattgacc acaaatgaac
     1501 ccaatatacc ggaaacaccc gtcccataaa ctgcaacatg tgttattgat tcgtcggcgt
     1561 cattgcttat gatggtgacc gaatccgcat aactgctaaa tccatccggc aagaaacgaa
     1621 caaccagttt gacagaatca cccggagtta aagtgagatt attgggcaga gtatccacaa
     1681 tcgtaaattg ggagctcccg ttctcaattg aaagcgaagt aatatccaga gcgccggcgc
     1741 caatattttt tacaaatact gtatctatct gaccgttacc actaaatatt ccaccgtaat
     1801 ctaagtacgt gccggtcaaa gtatatacga cgatctccgg ctgtggtgca aatggcatcg
     1861 aaaatgccga ggtaccctgc gcactatcct gcatggccgt aatcgcagta cccgcaggaa
     1921 tatttacttt caatgaccag tcaccggatg cattggccgt cgtagtaccc agcttgaacc
     1981 ggccctgatt gatcgaatcg gcatacactt gaataaaggc attcggtgaa gcaataccat
     2041 acacggtcga gtcagcgcct acggatataa tctgtggcgg agtaatatcg ctttgagtac
     2101 cagcctgcaa ataaaaaccg tttccgtttc cgaaaatcac gttatcatga acgatgatac
     2161 tgtccgttcc tgaattaagc atcgcaattg cattggtatt gctaaaagca atcgtgtttc
     2221 ccatgatcga atcgcgcgaa gcaccgttgc tcagattgat tgcatcgctg ctattaccca
     2281 agttttgagt gcccgtgaca tccgtaccga tatagtttcc ttgaacttta ttttcagaac
     2341 tattgataaa ccaaacaccg tagttggtac tggctgaaat tatatttcgt tcagctgcgg
     2401 tagcgccacc gatcaaatta agtcgcggag aaccggaacc accttcagaa atcgtcacac
     2461 cgataccgtt gccgagtggc accgttccgt tgacatcagt cccgataaag ttaccctgta
     2521 ccaaattggt atctgagcct acgttcgtaa tttcgatacc gctgtttgtg ttaccggaaa
     2581 tgacatttcg tgctccggcc accgtaccgc cgaccagatt ggcatggccg ctattgagta
     2641 ctacgcccga tccgttggat atagctgcat tacccgtcgc attggttcca atcagattgc
     2701 cttgtatgat ttgacctctt gtgccaatat tgccgatggt aatacctgaa ttattgttac
     2761 cagatattac gttaccggct cccggcgatg tgccgccgac gatattatca tgcgcttgat
     2821 tgagaataaa cacgccactc tgcgtattgc caagatcaac attcccgttt atatcggtac
     2881 cgatatagtt accttgtacc acattatgcg cagaaaatcc ctgcatcaaa ataccgctgt
     2941 taccgttgcc ggaaataacg ttgcgatgat tggacgtcat accaccgatc gcggtatgca
     3001 tggaattatt atcaagtgca ataccccatg aaccgttgcc gagatcagac gtaccgttaa
     3061 tgtccgttcc gatgagattt ccccaaacgt aattggaatc tgtgcccgca tccgtaatga
     3121 gaataccggt attagtatta ccggagataa cgttttgatt tacgccaagg gtatcaccga
     3181 tctcgttgta cttcgaaccg ccgatcaggg aaatgccgat gaggttagga taaccgatga
     3241 taccggacgc atctgttccg atcaaattat cctgtacttt attataagaa ctaccggcaa
     3301 tcaatatacc cgactgcgga tgattggaaa tcacattacc gatgatctga cttccgccgc
     3361 cggcattcaa agcgataccg tgatagttat tggggatcgt agaattatcc gaaccgatac
     3421 cgatgcgatt accgcgaatc gtattaaggg gacccgccgc ataaataccg gtactgtcat
     3481 tgccggcgat aatattacta tcgacggtcg ccattgcaaa aatatacaca cccagatctt
     3541 gattgcccaa tccgatggtt ccggtagcat ccgtaccgat acggttatta cgtatcaccc
     3601 agtttgcacc ggtcgaacca acaccgcccc aagcattgtt atagatcgta ctgttgatca
     3661 cggagtcatt actcgtttga ccgatgatac cataacgctg agcattacga actgtgagat
     3721 tttctgcttt actgttatca cctgccagac ctgcatcatc aaagaaaatt ccaaaacgcg
     3781 cctgtgccga accgttttgt gcatccagcg tcatatttcg aatcgttacg ttcggtgatt
     3841 gaacactaaa tattgtgtta tcgccaaatg ggaacgacac gctcacctgt accgtgttgt
     3901 tatttgtttc accgtcaata acaaccggcc ccgacgcatc atttatatca agatccgaat
     3961 caatgaatat tgtagcattg gaaattgcaa aacgaatact atcagcgccg ggtaatgatg
     4021 cggcggcagc aatcgctgcg cgcaatgatc cttcaccggc atcagccgtg tttttaacaa
     4081 gtaagggatc aaaatcagcg gaaacatcag aagaataaac gcctgcctga tcggcactgt
     4141 ccacagcgct tacgcgataa taataaatat tcccgaccac tgcattcaca tccacaaacg
     4201 ctgtatctcc gacggctgtc tgatcaatcg aatccgtaag ggacggaata aatccgggcg
     4261 tggtgctccg ataaataatg tatttcttca aatccgtctc agaattatga ttccacgtta
     4321 ttcgcaccga atccgggaaa aacgtcgtag ccgaaattcc agccacggcc gtcggcgcca
     4381 tcgctgtaga agttacaaaa tcagcgccat taactaggtt gccgttgtat gcattgggtg
     4441 aggcatcaag agctgtagtc gaagcagacg gttcatccaa atgataaagc gctacaagcc
     4501 ctacttcatc gccgcgatac ggtttggcca aaccggaagt gatttgtgcc ggtgccagcg
     4561 cataattcca aattgacaat tcatcaatct gaccatcaaa ggtaccccac ccgttattgt
     4621 aaccgccaat ccatgcatta actcctgaag tggttcttga ataggcaccg ttggcctgtt
     4681 caacaccatt cacgtagagt cgggctatgc ttccatcgag cgttaaagcc acatgggtcc
     4741 acgaattgat cggaatctgc cccgatgatg tggatacagc acctgatgga aagccgtatt
     4801 catagtataa tgtaccatcc ggcaaaagcc ccaatgcaaa gccgtcttca ttggtaccgg
     4861 aacttccacg attagaccaa attgcgtgtt gttgagccga agagggtttt atccaagcgg
     4921 atatcgtcat agtaatattt tcgaatgtac tgggtagcgc aacatactca gaattaccag
     4981 cctcaagagc caacgcattg ccggattcaa caaccgaaac ggcggcatct tgagtcgaat
     5041 aatttccaat ttgtcccgca ctgtcctcac ccgcaacacg gaagtagtac atctgtccgt
     5101 ttactaaacc cgttgcaata aaggtagtgt cgttgacatc accatctgtc gttgtaccga
     5161 tatccgttgt tgggttagcc gtgatgccgc tataaacatg gtaacggaag aaatcggcat
     5221 ctgtattttt gttccacttg agttcaactt gaccattatg cgcaatcgcg taaaccccgg
     5281 caggcgctac aggagccatg gcgccggacg gcgtgaaatt attaccttga ccattgttaa
     5341 gtacgccatc attcgcagaa gttgaacctt catacacaat tgttcccgga tcactttcat
     5401 caaaatgcca caggttcatt aacccgacct catctccacg aagcgacttt gtcatttgat
     5461 cactgatttg cgattgcgaa cgaacatgat tccaaatacg caattcgtct aagcttccgt
     5521 taaaaggtcc acccagcgtc attgactcgg caccgatgta cagtttaccc tggcccgtat
     5581 atataccgga gaagtttgat gtttcgtatt taccgtcaat atacaaaaca cgttcgccgg
     5641 tacttcgatt ggctgtaaat gcgatatggt gccaattatt atcgaatgtg tatgaactct
     5701 gaatacgatt gttccagaat tccatataaa tattactagt aacttcaaaa ccgatatcga
     5761 ggaaattatt cgtactagcc ggcaaagcgc cttgagaaat gatcaggttt ccgacggcat
     5821 tacgtttgac ccaaaattct atagtgaagt cggttgaaac caaatctacc gagtcagcca
     5881 agatataatc gtcaacgcca tccaatacca acgcatttcc ggcttctata accggcacga
     5941 ccgcatcttc attggagaat gc
//
LOCUS       DAOQGF010000166         5927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6743_length_5927_cov_7.141860, whole genome shotgun sequence.
ACCESSION   DAOQGF010000166 DAOQGF010000000
VERSION     DAOQGF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6743_length_5927_cov_7.141860"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..606
                     /locus_tag="PK937_14785"
     CDS             82..606
                     /locus_tag="PK937_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33889.1"
                     /translation="MKTKDVSVVTTYLAMHQKPSIETLAMPPDCVILRAENPTVKFYR
                     FLYDAVGADWNWIDRKKMADEVLAEETQDALVEIYVLYDRGVPAGYAEIDRRIAGEIE
                     LKYFGIIPEFISKRYGRFLLTWIIEKAWQYKPQRFWVHTCSLDHPRALPLYQNLGFEI
                     YKTETHTVTIPEVL"
     gene            603..1001
                     /locus_tag="PK937_14790"
     CDS             603..1001
                     /locus_tag="PK937_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNH33890.1"
                     /translation="MMRYVLLWVFMASVLCAQDEKKPAEKNTGKKQFVYVLKLRPDLL
                     KEENWKEADNAAVGRHFNYLKNLLAEGTLIFAGRTDVMNENTFGIAVFWATDESEAQK
                     IMAGDAAVQAGVMTAQLLPFKVALMQNNNK"
     gene            1031..1585
                     /locus_tag="PK937_14795"
     CDS             1031..1585
                     /locus_tag="PK937_14795"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNH33891.1"
                     /translation="MTHDERGILIKKIKDLPKNLEKAIAGLNKKQMDTPYREGGWTPR
                     QVVHHIADSHMNAYIRAKLAVSEDHPTIKPYDQDAWAEMEDGKSLPVDYSMTIIKALH
                     ARWGQFWDRVQESDWSRTVMHPANGVMSLERILLTYANHGEKHCEHINGLRVRMGWTG
                     KNKPTKKAKAKQVKKTSKNKRKRG"
     gene            1582..2040
                     /locus_tag="PK937_14800"
     CDS             1582..2040
                     /locus_tag="PK937_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017033399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH33892.1"
                     /translation="MNIIRCEERHLDKLVPLFDAYRMFYHQSSDESAAKIFLSARIRN
                     GESVVFIALDTNENAVGFTQLYPLFSSVSMQPVWLLNDLYVSQSARRSGVGEALMEKA
                     RRFAVETGAKGIELETGVENVQAQALYEKLGYQRNTDTYRYFLPVTAALK"
     gene            2149..2718
                     /locus_tag="PK937_14805"
     CDS             2149..2718
                     /locus_tag="PK937_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33893.1"
                     /translation="MLRMTLTVSCVLILIGCGEDKTSSEKEIFHRSLNDRAVKYLSPQ
                     TVDLDSNGAEDYSFTTSLMQEDGKTVLQFVINGTTSNAVLYNTSSAEIPALAQNTLIS
                     SSPASPKQWDAFENDLCSILYTSATDSVWRGNWSKANHQFVGVRFTVSDQHHYGWISM
                     SADTTNGQLILHACAFNTTAGGSIKAGEK"
     gene            2753..3229
                     /locus_tag="PK937_14810"
     CDS             2753..3229
                     /locus_tag="PK937_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNH33894.1"
                     /translation="MMESKHWPEVAAIYQAGIETGNATLETSVPSWEAWDKAHIPQCR
                     FVVIDNNIIAGWAALTPVSGRCVYAGVAEVSVYIHPAYKGKGIGKKLLDQLALSSEAQ
                     GLWTLQAGILRENTASLKLHAACGFREIGYREKLGQYQGVWRDVVLMERRSKNVGQ"
     gene            3279..3791
                     /locus_tag="PK937_14815"
     CDS             3279..3791
                     /locus_tag="PK937_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNH33895.1"
                     /translation="MRPTKDEYPSFFETYVSKVPDGDIVQQMRQGVENTVKLISPLSD
                     DLLNYRYAEGKWSIKEVLMHLSDAERIFSYRALRFARKDKTDLPGFDENIYVPASKAT
                     QRTAESILNEYQTVRAASITLFESLDDEALQERGTANGRTTSVRAMAYILVGHELHHV
                     GIIKERYLKK"
     gene            3815..4246
                     /locus_tag="PK937_14820"
     CDS             3815..4246
                     /locus_tag="PK937_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNH33896.1"
                     /translation="MKSFYFPMMLTVASNVIYHIAQKSIPAHLNPIIPTIIAYAVALT
                     TSLCLYPFFSDTHLTQAGWRDVNWATLAVGMAIVGIEIGFLLAYRAGWNISLGAVTSN
                     VTVTLLLLPIGILFFKEHLSWEKITGIVLCMGGLWLIFKPS"
     gene            4262..5407
                     /locus_tag="PK937_14825"
     CDS             4262..5407
                     /locus_tag="PK937_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNH33897.1"
                     /translation="MQKKLSDDSLCIHAGTKRSGEFPGVNTPIYTSTAFEYKTAGPMV
                     YPRYYNTPNQQAVIEKIAALEKAEAGVLFSSGMAAISTTMLTLARKGDHILLQYDIYG
                     GTYHFAATQLEPLGIEYSFVRSLDPAEYEKAIRPNTKLIYIETPNNPLLKIIDIEAIA
                     AIGKKHGVTTMIDNTFASSVNQNPIALGIDIVMHSGTKYLGGHSDICCGVVVTREALA
                     DAIRKKANMFGGSLNAETCYLLERSIKTLSVRVDRHNHNAMTLAEALQNEKRIKRVYY
                     PGLASHDGHAVAKRQMRGFGGMLSFDIDTAVISPDQFLEQLRLIKPALSLGGVESTLC
                     MPSKTSHLRLSAEEKKQAGITDALMRLSVGIENATDILDDIREALRS"
     gene            5404..5787
                     /locus_tag="PK937_14830"
     CDS             5404..5787
                     /locus_tag="PK937_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNH33898.1"
                     /translation="MKTKLRIQNGPAALSVVRLASDAPWPLWLQSKDGFFSVTRTDEE
                     LSIVCKQILVPPDARAEHGWRAFKIVGPIPFEMTGVLASVIAPLGEAGISIFAVSTFD
                     TDYILVKEENWSPARAVLDDVVEWI"
BASE COUNT         1681 a         1249 c         1503 g         1494 t
ORIGIN      
        1 taatgtaacc gcgaaacccg aacggttgat ggaaatcgta gatatggata tctattcaaa
       61 aatcgctaag ggaaatcgtc tatgaaaacg aaagacgtaa gcgtcgttac gacgtacctc
      121 gcgatgcatc aaaaaccgtc catcgaaact ctagcgatgc cgcccgattg tgtgatcctt
      181 cgcgctgaaa atccgacggt aaagttttat cggtttttgt acgatgcggt gggtgcggat
      241 tggaattgga tagatcgaaa aaaaatggcg gatgaggtat tggccgaaga gacgcaggat
      301 gcactggtgg aaatttatgt tctgtatgat cgcggtgtgc cggcaggtta tgcggagata
      361 gatcgtcgca ttgccgggga gatcgaattg aaatattttg gcatcatacc ggaatttatc
      421 agtaaacgtt acggccgctt tttgctgacc tggattatcg aaaaggcgtg gcaatataag
      481 ccgcagcgtt tttgggtgca tacgtgttcg cttgatcacc caagagcttt accgctgtat
      541 caaaaccttg gatttgaaat atacaaaact gaaacacaca ccgttaccat cccggaggtt
      601 ctatgatgcg ttatgtgttg ctatgggttt ttatggcgag cgtactttgt gcgcaggatg
      661 aaaaaaagcc ggccgaaaaa aataccggta aaaaacaatt tgtgtatgtc ctcaaacttc
      721 gaccggattt actcaaagaa gaaaactgga aagaagcgga taacgccgcc gtcggacgtc
      781 atttcaatta cctcaaaaac ttgttggctg aaggtacttt gatttttgca gggcgaacgg
      841 atgtgatgaa tgaaaatacg tttggtatcg cggtgttttg ggcgacggat gaaagtgagg
      901 cgcaaaaaat catggccggt gacgccgctg tgcaagcggg cgtaatgaca gcccaactac
      961 tgccattcaa agtggcgttg atgcaaaaca ataacaaata atgtcggctg taaaaataaa
     1021 aggaaccgat atgacacacg atgaacgtgg gatactgata aaaaagatca aagacttacc
     1081 caaaaactta gaaaaagcca tagccggctt gaacaagaaa caaatggata cgccgtatcg
     1141 tgaaggcgga tggacgccgc gtcaggttgt gcatcatatc gccgattccc atatgaatgc
     1201 ctatatccgg gcaaaactgg ctgtgtcgga agatcatccg acgatcaaac cgtacgatca
     1261 ggatgcctgg gcggaaatgg aagacggcaa gagtttaccg gttgattatt cgatgacgat
     1321 catcaaagcg ttgcacgcac gatggggaca gttttgggat cgcgtgcaag aatcagattg
     1381 gtcgcggaca gtcatgcatc ccgcaaacgg tgtaatgtca ctggagcgta ttcttttgac
     1441 gtatgccaat cacggtgaaa aacactgcga gcacatcaat ggtttgcgtg tgcgtatggg
     1501 ctggacagga aaaaacaaac caaccaaaaa agcaaaagca aaacaggtaa aaaaaacatc
     1561 gaagaataaa aggaaacgcg gatgaatatc atacgctgcg aagaacggca tttagacaaa
     1621 ttagtaccac tttttgatgc gtatcggatg ttttatcatc aatcgtccga tgaatcagcg
     1681 gccaaaattt ttttgtcggc ccggattcgt aacggggagt ccgttgtttt tatagccctg
     1741 gatacgaacg aaaacgccgt tggttttacg cagttgtatc ctcttttttc atcggtgtcc
     1801 atgcaaccgg tttggctgct taatgattta tacgtgagcc aatcggcgcg ccgatcgggt
     1861 gtcggtgagg ctttgatgga aaaagcacgt cgctttgccg tcgaaacagg cgctaaaggc
     1921 attgaactgg aaacaggtgt tgaaaatgtt caagcgcagg cgttgtatga aaaattaggt
     1981 tatcagcgca acacggatac gtatcgctat tttttacccg taacagcagc gttgaaataa
     2041 agttcaaaaa acgcgtacta agaaaatatg cagatatgat ataagtacag gttaaaaaag
     2101 ttatgtttta caatttacta tttctaagta actaaatcgg ggaaacgtat gttacgaatg
     2161 actctaaccg tatcgtgtgt tttgatattg atcggatgcg gcgaagataa aacttcgtca
     2221 gagaaagaaa tttttcatcg ttcgctcaat gatcgtgccg tcaaatatct gtcaccgcag
     2281 acggtggatc tggatagcaa cggtgcggag gattattcct tcaccacatc gcttatgcag
     2341 gaagacggaa aaaccgtgtt gcagtttgta atcaacggta ccacttccaa tgcggtgttg
     2401 tacaatactt catcagcgga gatacccgct cttgctcaga acacgcttat ttcatcgtcg
     2461 cccgcaagcc ctaaacaatg ggatgcgttc gaaaatgatt tgtgttcgat cctttataca
     2521 tcagctaccg attccgtctg gcggggcaat tggtctaaag cgaatcatca atttgttggc
     2581 gtacgtttta ccgtcagcga tcaacaccat tacgggtgga tcagcatgag cgccgataca
     2641 actaatgggc aattgatttt gcatgcatgc gcttttaata cgacggccgg tggatccatc
     2701 aaagccggag aaaaataaag tgaacgcgac aacaaataca tacgcattgc aaatgatgga
     2761 gtctaagcat tggccggaag tggccgccat atatcaggcc ggcattgaga cgggaaatgc
     2821 gacgcttgaa acatccgtac cgagttggga ggcatgggat aaagcacata taccgcaatg
     2881 ccgttttgtg gtgatagata ataatattat cgccggatgg gctgcattga cgccggtatc
     2941 cggccgttgt gtatatgccg gcgtggccga ggtgagtgta tatattcatc cggcttataa
     3001 gggaaaaggc attggaaaaa aactacttga tcaattggcg ctttcatcgg aagcgcaagg
     3061 attgtggact ttacaggcgg gaattttacg cgagaataca gccagtttga aattacacgc
     3121 cgcctgcgga tttcgtgaaa taggctatcg agagaaatta ggtcagtatc aaggcgtttg
     3181 gcgcgacgtg gtattaatgg aacgccgcag taaaaacgtc ggccagtaag ttattgaaag
     3241 acgtcatcaa taataagcca taacaaacag gagcgcatat gcgtccaacg aaagatgaat
     3301 acccctcgtt ttttgaaacg tacgtaagca aagtaccgga cggcgatatt gtacaacaaa
     3361 tgcggcaagg tgtagagaat accgtaaaac tcattagccc attgagcgat gatctgttga
     3421 actatcgtta tgccgaagga aaatggagta tcaaagaagt tttgatgcac ttatccgatg
     3481 ccgaacggat tttttcgtat cgtgcgcttc gttttgctcg taaagacaaa accgatttgc
     3541 cggggtttga tgaaaacata tacgtacccg cgtccaaagc aacgcagcgt accgcagaga
     3601 gtattttgaa cgaataccaa accgtgcggg ccgcttcgat cacgctattt gaaagtttag
     3661 acgacgaagc attgcaggag cgcggcacgg ccaacgggcg cacaaccagc gtacgtgcga
     3721 tggcttatat tttagtcgga catgaattgc accatgtggg catcatcaaa gagcgctatc
     3781 taaaaaaata aatcggctat taaccgagag atctatgaaa tctttttatt ttccgatgat
     3841 gttgacggtg gcatccaacg tgatctacca catcgcgcaa aaatccatac cggcgcatct
     3901 caatccgatc attccgacga tcatcgctta tgcggtagcg ttgacgactt cgttatgttt
     3961 gtatccgttt tttagcgata cccatctaac gcaagcgggt tggcgggatg tcaactgggc
     4021 cacgttggct gtcggtatgg cgatcgtcgg catagaaatt ggttttttac tggcttaccg
     4081 tgccgggtgg aatatcagtc ttggcgccgt gacatccaat gtgactgtga cacttttact
     4141 tttgcccata ggcattctgt ttttcaaaga acatttgtca tgggaaaaaa taaccggtat
     4201 tgttttatgt atgggcggct tgtggttgat tttcaaacct tcttaaaaaa aggaaattgt
     4261 gatgcagaaa aaattatcgg atgactcctt gtgtatccat gccggtacca aacgcagcgg
     4321 tgaatttccc ggtgtcaata caccgattta tacgtctaca gcatttgaat acaaaaccgc
     4381 cgggccgatg gtgtatccgc gatattacaa tacaccgaac caacaggcgg tcatcgaaaa
     4441 aattgccgca ttggaaaaag ccgaagccgg tgttttgttt agttccggta tggcggcgat
     4501 cagcacgacg atgctcacat tagcccgcaa aggcgatcat attttactgc aatacgatat
     4561 ttacggtggt acgtaccatt tcgcagcgac acaactggaa ccgctcggta ttgaatattc
     4621 gtttgtccga tcgctggatc ctgcagagta tgaaaaggca attcggccca acaccaaatt
     4681 gatctacatc gaaacgccca acaatccgct gctcaaaatc atagatatag aggcgatcgc
     4741 ggccatcggt aaaaagcacg gcgtcacgac gatgattgac aatacgtttg cgtcgtccgt
     4801 caatcagaat cctatcgcac tcggaataga catcgtaatg cacagcggta ccaagtatct
     4861 cggcgggcat agcgatattt gttgcggtgt ggtcgttacc cgcgaagcgt tagcggatgc
     4921 aattcgaaaa aaagccaaca tgttcggcgg tagcctcaat gccgaaacgt gttatctgct
     4981 ggagcgcagc atcaaaacgc tttcggttcg ggttgaccgt cataatcata atgcgatgac
     5041 gctggctgaa gcattgcaaa atgagaaacg tatcaaacgc gtttattatc ccgggctggc
     5101 atcgcacgac ggtcatgccg ttgcgaagcg tcagatgcga ggttttggcg ggatgttatc
     5161 ctttgatatt gataccgcgg ttatttcacc ggatcaattt ttagaacaac tgagattgat
     5221 caagccggcg ctgagtcttg gcggtgtaga atccacactg tgtatgcctt ctaaaacttc
     5281 acatctgcgc ctttcggccg aggaaaaaaa acaagcgggt attacggatg cgctgatgcg
     5341 attgtccgtc ggtatcgaaa atgccacgga tatacttgat gatattcgag aggcgttgcg
     5401 ttcatgaaaa ccaaactccg aatacaaaac gggccggccg ctttatcggt tgttcgttta
     5461 gcatcggatg cgccgtggcc tctctggctc caatcgaaag atggtttttt ttcggttaca
     5521 cgaacagatg aggaattgag tatcgtgtgt aaacaaatcc ttgtcccgcc ggatgcccga
     5581 gctgaacacg gttggcgtgc ttttaaaatc gtgggaccga taccttttga aatgacgggc
     5641 gtattggctt cggtgatcgc accgcttggt gaagccggga tcagtatttt tgccgtctcg
     5701 acgttcgata cggattatat tttagtcaaa gaagaaaatt ggagccctgc acgtgcagtg
     5761 ctcgacgacg tggtagaatg gatataatat ttaaagatca tttttctggt catgcggagg
     5821 cctatgcgac gtttcgcccg cattatccgg aaccctttta tgcgtggctg ataagtctta
     5881 cggcgcggca agaaaacgcg tgggattgcg gcaccggtaa tgggcag
//
LOCUS       DAOQGF010000167         5826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_6905_length_5826_cov_5.130133, whole genome shotgun sequence.
ACCESSION   DAOQGF010000167 DAOQGF010000000
VERSION     DAOQGF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6905_length_5826_cov_5.130133"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..743
                     /locus_tag="PK937_14835"
     CDS             84..743
                     /locus_tag="PK937_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNH33899.1"
                     /translation="MKKIVIFGAGDIARLAHYYFKTDSNYEVVAFTVDREYVKSDNFL
                     DLPLVVFDDIEKKYPPSEFGMFVALSYAKLNKARAEKYVETKKRGYTLVNYISTRCSN
                     LTQFPIGDNCFILEDNTIQPYVRIGNNVTLWSGNHIGHDSVIEDHNFISSHVVISGHC
                     TIKSYCFLGVNATLRNGITIAEENIIGAGAIIMKNTKHKDVYLPAKSVLFDKQSDAVD
                     L"
     gene            806..1672
                     /locus_tag="PK937_14840"
     CDS             806..1672
                     /locus_tag="PK937_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013922628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33900.1"
                     /translation="MKTHAAVPFLNSIDANGVARIYFTSRDEQDRSSIGWFRINLNNP
                     RTILNISQEPVLRFSSQGYFDEDGVMGCQIIEWENAHLLYYIGWNKAVTVPFRNAVGV
                     AISKDNGLTFDRLYKGPILDRSIHDPCFVASLCVLNTGMKYQMWYLSCIDWLKKNDLL
                     LHKYHIKYAESHNGIDWIRNGAIAIDFASDSEYAISVPRVIVEEKKYKMWYSFRATQA
                     NKNYQLGYAESDDGIRWKRIDNVNFIGLHEEWDSEMQCYPFVFKYNQRTYMLYNGNAY
                     GKTGIGLALLDQ"
     gene            1681..2598
                     /locus_tag="PK937_14845"
     CDS             1681..2598
                     /locus_tag="PK937_14845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH33901.1"
                     /translation="MMISIVSPVYNAASIIPKLIEALTHELEQITKEYEIILVDDRSP
                     DTSWQVMYNAAQENKNVKCLRLSRNFGQHYAVTAGLSAASGDVIILMDCDLQDDPIYI
                     KVLFNEFQNGSEIVYSQRIERKHKLFKRIGAFTFNILFRIFSDKQYDIQFGSMTLITR
                     KVQKAFLEIRDQDRLYIQILKWLGFKSSYVAIEHKQRFAGKSSYSISKLISMAFQGWT
                     SHSTRLLKLSIYSGIIFSSFAFIGIFLALYQYFLYNIQPGWTSLIILILLSTGMIQLS
                     IGIVGLYIGKVFNQSKNRPLYIIDESINF"
     gene            2612..3706
                     /locus_tag="PK937_14850"
     CDS             2612..3706
                     /locus_tag="PK937_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNH33902.1"
                     /translation="MLRLGIVGGNRGFLIHQLCNHIKDRIKITAVADVAEDALSQWKH
                     LDETIQRYNSLEQMLTHAHINAVYICTPAPDHANQAVKALISGLHVLSAVPACMTIDE
                     CFSLIKTARKSDKMYMLAENYCYIPENKWINEQCHQGNIGEITYIRGAYIHDCKSLMF
                     SGKDLTWRGELSRTLVGNVYPTHTIGPIAQWLDINKHDGDRFKSIRSFTTAQKSLAAY
                     AASKFGNGHPYASNDFFCRGDMSFSLIETEKGRIIELIYDTHSNRPASKSEYYIQGTK
                     ACYISGRYTGESAIFWSAASEDAVFQNVNLPSDTYDPDLNLLRDFINAIETETPSPIG
                     VLDAVTWSSIIPLSEKSINNNNEAISFPEF"
     gene            3717..4844
                     /gene="rffA"
                     /locus_tag="PK937_14855"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             3717..4844
                     /gene="rffA"
                     /locus_tag="PK937_14855"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNH33903.1"
                     /translation="MRHIGFNKPYLTGKELEYIRQSVESGKISGDGVFTKKCHAFFEQ
                     RYGFKKCLLTTSCTDALEMAAILIEAGPGDEIILPSFTFVSTANAFALRGAKLIFADS
                     EASTPNIDATKIEPLITSRTKAIVVVHYAGVACDMNTIMETAKKHNIFVIEDAAQAID
                     SYYEGCPLGSIGHLATFSFHETKNVIAGEGGMLVINDERFIKRAEIIREKGTNRSAFF
                     RGEIDKYGWVDIGSSFLPSDIIAAFLYAQLEHIDEIQKKRKGLWNHYYTLLTFLEDKH
                     RVKLPFIPQYATNNAHMFYLVFSDTATRTTVIDLLKQNNIHAVFHYQSLHVSAFYKHN
                     NDGRDLPLSDRYTNTLLRLPMYYDLEREDVELIAKIIKSIA"
     gene            4892..>5826
                     /locus_tag="PK937_14860"
     CDS             4892..>5826
                     /locus_tag="PK937_14860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH33904.1"
                     /translation="MPTFFSERVGGVEIQTYLLATHLSKLEWKVDFVSETYDVRKIGR
                     SEIFDGVTIHWVSKKNHFSFWRRDIQKLVLLIKPDYIYQRGRSRFTSSPIAINVAKKL
                     KAKLIYHCAEDNDFNRSFNFEQVHQSTKSFFKKIILGFEALLSDYYFYQTIKKSHLII
                     AQTESQKLQFLRFFNKDATVIRSAHELPNEVIQKSEPPMIFWIANTGRRKQPEKFIEL
                     AKSLLDTPYRFVMAGPIPDRGYAEELLNQIRSTPNLEYAGPLSWEESNNFFAKATFFV
                     NTTLLGREGFPNTYIQAWMRGTPVLTLHCDPDGLI"
BASE COUNT         1841 a         1124 c         1113 g         1748 t
ORIGIN      
        1 aaacaaaatt tgcaaaaaat attgctctgt tacatgatta tgatctgtac gaatttacca
       61 ttcttgttag aaagtaaaat tatatgaaaa aaatagttat atttggcgcc ggcgatattg
      121 cacggctcgc tcattattat ttcaaaacag acagcaacta cgaagttgtg gcatttacag
      181 tagatcgtga atatgtaaag agtgataact ttcttgattt gcctttagta gtcttcgatg
      241 atattgaaaa aaaatacccg ccttcggaat tcggaatgtt tgttgcttta agttatgcaa
      301 aacttaacaa agcacgcgct gaaaaatatg tggagacaaa aaaacgaggt tatacattag
      361 ttaattatat tagcacccgc tgctcaaatc tcactcaatt tccaattggc gataattgtt
      421 tcattttgga agacaatact attcaaccgt atgtccgcat aggaaacaac gttacattgt
      481 ggagcggtaa tcatatcggg catgactcgg tgattgaaga tcataacttc atcagttcac
      541 acgtcgtcat ttcagggcac tgcactatca agtcgtattg ctttttgggt gtcaatgcga
      601 ctctccgcaa cggcattacg atagccgaag agaatatcat cggtgctgga gccatcatca
      661 tgaaaaacac aaaacacaaa gatgtatatt taccggcaaa atccgtttta tttgacaaac
      721 aaagcgatgc agtagatctg tgagcactca acaaaagtgg attaaactag gcagaatatt
      781 ttgcccgttc agctcatacg aatggatgaa gacccacgct gccgtgcctt tccttaattc
      841 tatagacgcc aatggcgttg cgcgtattta ttttacaagc agggatgaac aagatcgatc
      901 ttcaatagga tggtttcgaa tcaatttaaa caaccctcgg acaattctga atatttcaca
      961 ggaacctgta ctgcgatttt cctcgcaggg ttattttgat gaagatggtg taatgggttg
     1021 tcagattatc gaatgggaaa atgcacatct cctgtactac attggctgga ataaagccgt
     1081 tacagtaccg tttaggaatg cagtcggcgt agcaatcagc aaagacaacg gtttaacatt
     1141 tgaccggttg tacaaaggac ctattttaga tagaagcatt catgatcctt gttttgtagc
     1201 cagtttatgt gttttaaata ccggaatgaa ataccaaatg tggtacttat catgcattga
     1261 ctggttaaag aaaaatgatc ttttacttca caaataccat attaagtatg ctgaatccca
     1321 caatggaatt gattggatac gtaatggggc aattgcaatt gattttgcat ctgacagtga
     1381 atacgctatc tctgttccga gagtgatcgt agaagaaaaa aaatataaaa tgtggtattc
     1441 atttcgcgct acacaagcca ataaaaacta ccaattgggt tatgccgaat ccgacgatgg
     1501 gattcggtgg aaaagaattg acaatgttaa ttttatcgga ttgcatgaag agtgggattc
     1561 tgaaatgcaa tgttatcctt tcgttttcaa atacaatcaa cgaacatata tgctttataa
     1621 cggcaatgcc tacggaaaaa ccggtatcgg gcttgcatta cttgaccaat agtttatcac
     1681 atgatgatat ccattgttag tcccgtctat aatgcggcct ctatcattcc aaaattaata
     1741 gaggccctca cgcacgaact cgaacagatt acaaaagaat atgaaataat actcgttgat
     1801 gatagaagcc ctgacacgtc ttggcaagtc atgtataatg ctgcacaaga aaataaaaat
     1861 gtgaaatgct tacgcctatc ccgtaatttt gggcaacatt atgcggtaac tgccggtcta
     1921 tcagctgcaa gcggcgatgt gattattcta atggattgcg atttgcagga tgacccaata
     1981 tatatcaaag ttctatttaa tgagtttcag aatggctcgg aaatagtata ttcacaaaga
     2041 atagaacgaa agcacaagct atttaaaaga atcggggctt ttacattcaa tatcctattt
     2101 cgaatatttt cagataagca atacgatatc caatttggaa gcatgacgct aataacgcga
     2161 aaagttcaaa aggcttttct agagataaga gatcaggata gattatatat tcaaatttta
     2221 aaatggcttg gttttaagtc atcatacgtg gcaattgagc ataagcagcg ctttgcagga
     2281 aaatccagtt attctatatc aaaactaatc tcaatggcct ttcaaggctg gacatctcat
     2341 tcaactcgcc tactcaagtt atctatttat tccggaatca ttttttcaag ttttgcattt
     2401 ataggcattt ttcttgcgtt atatcagtat tttttgtaca acattcaacc agggtggaca
     2461 agtctaatta ttttaatact tttatcaaca ggaatgattc agctaagtat aggtattgta
     2521 gggctataca ttggaaaagt atttaatcaa agtaaaaaca ggccattgta cattattgat
     2581 gaatccataa atttttaata gatattaatg tatgctcaga cttggaattg ttggtggcaa
     2641 taggggcttt ttgatccatc agttgtgtaa ccatattaaa gatcgaatca agattacggc
     2701 cgtagcagac gttgctgaag atgctttatc acaatggaag caccttgatg aaactataca
     2761 aaggtacaat tccttagaac aaatgttaac acatgctcat attaatgcgg tttatatatg
     2821 tacacccgcg ccggatcatg caaaccaagc cgtaaaggcc ttgatttccg gtttgcatgt
     2881 tctatctgca gtacccgcat gtatgaccat agatgaatgt ttttcactaa taaagaccgc
     2941 tcgaaaatct gataaaatgt atatgcttgc agagaattat tgttatatac ccgaaaacaa
     3001 atggatcaat gaacaatgcc atcaaggcaa tattggtgaa atcacataca ttcgcggagc
     3061 atatattcat gattgcaaat cattaatgtt ttcaggaaaa gatctgacat ggcgcggtga
     3121 actctctcgt acgcttgtcg gaaatgtata tccaacacat accataggtc caatcgcaca
     3181 atggcttgat ataaataaac atgacggaga tagatttaag tcaatccgtt cgttcactac
     3241 ggcacaaaaa agcttagctg cttatgcagc ttccaaattt ggaaacggtc acccttacgc
     3301 ttcaaatgat tttttttgcc gcggtgatat gagtttttcg ctcatagaaa ctgaaaaggg
     3361 cagaataatt gaattgatat acgacaccca ttcaaaccgc cctgcttcaa agtctgaata
     3421 ttatattcaa ggtacaaagg catgttatat ttccggacga tatacaggcg aaagcgccat
     3481 tttttggtct gcagcatctg aagatgctgt atttcagaat gtcaacctcc catcagatac
     3541 ctatgatcct gatctgaatt tgttgagaga ttttatcaat gcaatagaga ctgagacgcc
     3601 ttcacccatt ggggttttgg atgctgttac atggagcagt attataccgc tttctgaaaa
     3661 atcgattaat aacaataacg aagcaatatc atttcctgag ttttgatata acttatatga
     3721 gacacatcgg attcaacaaa ccatacctca ccggcaaaga actagaatat atccgccaat
     3781 ccgtagaatc cggaaaaatt tccggagacg gtgttttcac caagaaatgc catgcttttt
     3841 ttgaacaacg gtacgggttc aaaaaatgtc tgctcaccac atcttgtact gatgcacttg
     3901 agatggcggc tattcttatc gaggccggac cgggtgatga gattattttg ccttcgttta
     3961 cttttgtatc taccgccaat gccttcgcat tacgtggcgc caaattgatt tttgccgata
     4021 gcgaagcttc tactcctaat atcgatgcga caaaaatcga accgctaatc acctcgcgca
     4081 caaaagccat cgttgtcgtt cactatgcag gtgtagcctg cgatatgaat actattatgg
     4141 agacagcaaa aaaacacaat attttcgtca tcgaagacgc cgcccaagcg attgattctt
     4201 attacgaagg gtgcccgtta ggttccatcg gacatcttgc gactttttca tttcatgaaa
     4261 ctaaaaatgt tattgccggc gaaggtggta tgctggttat caatgatgaa cgttttataa
     4321 agcgcgctga aatcattcgt gaaaaaggta caaaccgctc agcttttttt cgtggtgaaa
     4381 ttgataaata cggatgggtt gatattggtt cttcatttct cccttcggac atcattgccg
     4441 catttttata tgctcaactc gagcacatag atgaaatcca aaaaaagaga aaaggtttat
     4501 ggaatcatta ttacacacta ctaacgtttt tggaagacaa gcatcgcgtt aaattaccat
     4561 ttatccccca atatgccaca aataatgcgc atatgtttta tttggttttt tctgatacgg
     4621 cgacgcgtac gaccgtgatc gatttactaa agcaaaacaa tatccatgcg gtatttcatt
     4681 accaaagcct gcacgtttct gcattctata aacacaacaa cgatgggcgt gacctccccc
     4741 ttagcgaccg ttacacgaat acgctgcttc gtttacccat gtattatgat ctggagcgtg
     4801 aggatgtcga gctaattgcg aaaattatca aatctattgc ctaataacat tcacaacctc
     4861 aatgaataaa gatcagaaaa taacttttct catgcctact tttttttctg agcgcgtggg
     4921 tggggttgaa atacaaactt atttattggc gactcacctt tccaagttgg aatggaaagt
     4981 agactttgtt agtgaaacat acgatgtacg taagattgga agatcggaaa tatttgatgg
     5041 agtgacaata cattgggtta gtaaaaaaaa tcatttttcc ttttggcgcc gcgatattca
     5101 aaaactcgta cttttaataa aaccagatta tatataccaa cgtggccgct ctcgcttcac
     5161 ctcctcgcct attgccatta atgttgcaaa aaagttgaaa gcaaaactta tttaccattg
     5221 cgctgaagac aatgacttta atagaagctt taattttgaa caagtgcatc agagtacaaa
     5281 atcatttttc aaaaaaatta ttctcggttt tgaagcctta ctttcagatt attactttta
     5341 tcaaaccatt aagaaatctc atttaattat agcccaaaca gaatcacaga aacttcaatt
     5401 tttacgtttc tttaataaag acgccaccgt tatccgttcc gctcatgagt tacctaatga
     5461 ggtgatacaa aaatcagaac cacccatgat tttttggata gccaatactg gccgtcgcaa
     5521 acaaccggaa aaattcattg agcttgccaa gtcgctctta gatacaccgt atcgttttgt
     5581 aatggccggt ccgattcctg atcgaggata tgccgaagaa ttattaaatc aaattcgatc
     5641 tacacccaac ttggaatatg cgggaccgct ttcgtgggaa gaaagtaata atttttttgc
     5701 gaaggctact ttttttgtca acactacttt gctcggccgc gagggttttc ccaatactta
     5761 tattcaggct tggatgcgtg gaacaccggt tttgaccctc cattgtgatc cggacggact
     5821 gattga
//
LOCUS       DAOQGF010000168         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_7198_length_5651_cov_4.390457, whole genome shotgun sequence.
ACCESSION   DAOQGF010000168 DAOQGF010000000
VERSION     DAOQGF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7198_length_5651_cov_4.390457"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PK937_14865"
     CDS             complement(<1..691)
                     /locus_tag="PK937_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNH33905.1"
                     /translation="MNPKSQRLELTWIGKDDQPKLEPRILIEDPSKSFGDPKSENMLI
                     HGDNLLALKALEQDFASKIKCIYIDPPYNTGARIDADGQDVGYDDGIEHSEWLNMMKP
                     RLEILHKLLHKEGTLAVQIDDNEFARLYLLLSDIFGEKNLKVICVKMAEPTGVKMTHV
                     IRSGRIPKLKEYIIICRKGGIRNLLLEKIPKQKWDDEYKSVVIGISRKEIEELKEIIE
                     SESFTELDIQRG"
     gene            complement(797..1912)
                     /locus_tag="PK937_14870"
     CDS             complement(797..1912)
                     /locus_tag="PK937_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNH33906.1"
                     /translation="MSKPNEYFPQSVTHPGADLAEKLEEMDMGVKEFAIRTDKPEKTI
                     IAVIKGDSAITPDMAVQFEQVLKIPAHFWLNRQRQYDESMARTKQQEMIRQAEAWARS
                     FPLKEMLRLKWIRAYTNYGEAVTALFEFFGISNPSAWESLYLRSELKVQFRLSLAHTK
                     EHHAISAWLRRGEVQADTLDAPSYSEKAFKQELPEIKKIMAKHPKDFFVKLQTVCNEV
                     GVKVVHTPCLPKAPISGATRWLNDTPLIQLSGRYKRNDGFWFTFFHEAGHILLHGKKD
                     IFLEDVKYTDRDDEKEKEANLFAQKWVLNDAEKEEILHYARGVGKITHHDIVTFADKF
                     KTHPAMIIGQLQHDKLIPYHLGREFIEPVELDENGNC"
     gene            complement(1924..2256)
                     /locus_tag="PK937_14875"
     CDS             complement(1924..2256)
                     /locus_tag="PK937_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNH33907.1"
                     /translation="MNITFKSAKFAAIVNNDRKLLKELGKLRADKVRLRLNQLDAADT
                     LEVLRHTAGHYHDLTGDRKGQWSCDLDQPYRLIFEPRENPIPTNDAGQYIWSEIRGVH
                     IIEIIDYH"
     gene            complement(2457..5336)
                     /locus_tag="PK937_14880"
     CDS             complement(2457..5336)
                     /locus_tag="PK937_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNH33908.1"
                     /translation="MTPYHAKYFAYELTRRSASDSIQKLATALVDAQVDLNPHQVEAA
                     LFAFRSPLSKGAILADEVGLGKTIEAGLVISQKWAERHRKILIIVPANLRKQWSQELL
                     DKFFLPTVIIENASFNNAIRNGNLNPFDQKDIVICSYQFARKQEPYIKTIPWDLVVID
                     EAHRLRNVYKPTNKIANAIKNAVADTRKILLTATPLQNSILELYGLVSVIDDLHFGDL
                     KSFKMQYSRLGSEESDELYLELKQRLRPICHRTLRRQVLEYIKFTNRKAIVEEFYPSA
                     EEQKLYELVSEYLQRADLYALPKSQRSLMTLILRRLLASSTFAISGTLEALASKLDQA
                     TKQNGLPVPIGEQEFTDFEAIKEFEDEWQSDEEEESVAAPPKFTPEEIAGMKREMATL
                     QEFHTLAKSIKINTKGDKLITALKRGFEEAVGLGAQRKAIVFTESIRTQNYLKQILEQ
                     NGYAGQIVLFNGSNTDPESRDIYRQWIERNKNSDRITGSETADKRAAIVEYFRDTATI
                     MIATEAAAEGINLQFCSLVVNYDLPWNPQRIEQRIGRCHRYGQKYDVVVVNFLNKANA
                     ADQRVYDLLDQKFKLFSGVFGASDEVLGSIESGVDFEKRIAKIYQDCRTPDEIQLSFD
                     FLQKELEEQISGEMSKTKQKLLENFDEEVHEKLRINLEESSEYLSKYEQWLWDITSYF
                     LRDHANFSTTDHSFTLHTNPFPNEVIHNGPYRIGKNIDDANVYRIHHPLAQRVIDQCK
                     SAVLPLTELCFHYTGSEKKISILESLIGQSGWLRAFHISIESFEVEDRVFLVAYTDKG
                     ELLDIERCRRLFSLNADETKPMDSITSALSDEFEKAIYRLQTDFVDEVMRRNAGFLDA
                     EMEKLDQWAEDIKRSLEIELRNLDVEIKIVKAEARKVLKLEEKVALQRDIKEKEKKRN
                     TLRLSLYQQQDEVDKRKEQLIEDIEKRLHKGVERNELFTIRWSLK"
     gene            complement(5333..>5651)
                     /locus_tag="PK937_14885"
     CDS             complement(5333..>5651)
                     /locus_tag="PK937_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33909.1"
                     /translation="EQMNIFTRHHDLLKTLENDSDTFKIGARLLGRHMNSIMNSSVPL
                     ATRVTGILEKAIQDQQASKLATLMIRDMISSLEEAQTILQELLAGINDAPIEREDDKG
                     EGS"
BASE COUNT         1372 a         1314 c         1249 g         1716 t
ORIGIN      
        1 caccgcgttg aatatccaat tctgtaaagc tctctgattc aatgatctct tttaactctt
       61 caatctcttt tcttgaaatt cctattacga cacttttgta ttcatcatcc catttttgtt
      121 ttggaatttt ttcaagaagc aagttcctaa ttccaccctt acggcaaatt attatatatt
      181 cttttagttt tgggatacgg cctgatctaa tcacgtgcgt cattttaacg cctgttggtt
      241 ctgccatttt tacacaaatg acctttaggt ttttctcacc gaaaatatca gaaagtaaga
      301 gatacaacct cgcaaattca ttatcgtcta tctgaacagc cagagttcct tccttgtgta
      361 acaatttgtg taagatttct aatctcggct tcatcatatt gagccattca ctatgttcta
      421 ttccatcatc ataccctaca tcttgaccat ctgcatctat tctagcgcct gtattatatg
      481 gtggatcaat ataaatacat ttgattttgc tcgcgaagtc ttgttccaag gctttgagtg
      541 cgagcaggtt atcgccgtga atcagcatat tttcagattt gggatcaccg aagcttttgg
      601 atgggtcctc gatcaggatg cgcggctcca gcttgggctg atcgtccttg ccgatccagg
      661 tgagttcgag gcgttgggat ttagggttca tattggaatt tacttatagt atttgtagta
      721 tgacaaaaat gagccccggt ccacccgatg acgttgcgct tgttttttgc tccgaattca
      781 taactacgct ccaaaactag cagttgccgt tttcatctaa ttcaaccggc tcgataaatt
      841 ccctaccaag atgataagga attaatttgt catgttgcaa ctggccaata atcatagccg
      901 gatgggtttt aaacttgtcg gcgaaggtaa ctatgtcgtg atgagtaatt ttgccgactc
      961 cacgagcata atgaagaatc tcttctttct ccgcatcatt taaaacccat ttttgagcaa
     1021 agaggttggc ttctttttct ttttcgtcgt cacgatcggt atacttcacg tcttcaagga
     1081 aaatgtcttt cttgccatga agtaaaatgt gtcccgcttc gtgaaagaac gtaaaccaaa
     1141 agccatcgtt gcgcttgtag cggcctgata attgaattaa tggcgtatca ttgagccatc
     1201 gtgtggcgcc actgataggc gctttgggaa ggcacggcgt gtgtactacc ttgacgccga
     1261 cttcgttgca cactgtctga agtttgacaa aaaaatcttt agggtgtttg gccatgatct
     1321 ttttgatctc aggcaattcc tgcttaaaag ccttttctga ataagacggg gcatccagcg
     1381 tatcggcttg gacttcccct cgtcttaacc acgccgaaat ggcatgatgt tccttggtat
     1441 gtgcaagaga gaggcggaat tgaactttca attccgatcg aagatacagg ctctcccaag
     1501 ctgacgggtt ggaaatccca aagaattcaa acaatgcggt gacggcttct ccataatttg
     1561 tgtatgcccg aatccacttc agacgaagca tttcttttaa aggaaaggaa cgtgcccaag
     1621 cctccgcctg gcgtatcatt tcctgctgtt ttgtgcgagc catggattca tcgtattgcc
     1681 gttggcgatt gagccaaaag tgtgcgggga tcttcaaaac ctgttcaaac tggacggcca
     1741 tgtcgggcgt aattgcgctg tcccctttta tgacggcaat gatcgtcttt tcgggtttat
     1801 cggtacggat cgcaaattcc tttacaccca tgtccatctc ttcgagtttc tcagcaagat
     1861 cagcgcccgg atgggttacc gactgtggaa agtactcatt aggcttactc ataactcctc
     1921 ttttcaatga taatcaatta tctcaataat gtgcaccccg cggatttccg accaaatata
     1981 ttgcccggca tcattggtgg gaatggggtt ttcgcgtggt tcaaaaataa gcctgtacgg
     2041 ttgatccaag tcacaagacc attgcccttt acgatctccg gtgaggtcgt gataatgacc
     2101 ggctgtgtgg cgtaaaactt caagagtatc cgcggcatca agctggttaa gtcttaagcg
     2161 taccttatca gcccgaagtt taccaagttc tttcagaagt ttgcggtcat tattgacgat
     2221 tgcggcgaac tttgcacttt tgaatgtaat attcatgcgt taaaaagtca attataattt
     2281 acagatagtg taaatcaaat atatttaacc tacaaggata aaaaggctgt ggttcggcta
     2341 cgctcaccat gaaaccccac gcgcaggcat aagcctgcgg ctacaaataa actcactcct
     2401 caactactgc tttcgcgcct ctacacgctc gggacaggca cccgcggcta ccaaaattat
     2461 ttcaggctcc aacggattgt gaaaagttcg ttgcgttcta cgcctttgtg aaggcgtttt
     2521 tcaatatctt cgatcaattg ctccttgcgc ttgtccacct cgtcctgctg ttggtatagc
     2581 gatagacgca aagtattacg ttttttctcc ttttccttga tgtcacgttg aagagcaacc
     2641 ttttcttcga gtttcagcac tttacgggct tcggccttga cgatcttaat ttcgacgtcc
     2701 agattgcgca gctcgatctc aaggcttcgc ttgatgtcct ccgcccattg atccagtttt
     2761 tccatttccg catcaagaaa gccggcgttg cggcgcataa cttcgtccac aaaatcagtt
     2821 tgaagccggt agatggcttt ttcgaattcg tccgacaacg cggatgtgat agaatccata
     2881 ggttttgttt catcggcatt gagagaaaac aggcgccggc accgctctat gtcgagtaat
     2941 tctcccttat ccgtataggc cacgagaaaa acccggtctt cgacctcgaa agattcgata
     3001 gaaatatgaa atgccctgag ccacccggac tgaccgatta aactctcaag aatcgatatc
     3061 ttcttttccg atcctgtata atgaaaacag agctccgtta acggcaatac ggccgattta
     3121 cattgatcaa taaccctttg agccagagga tgatggatac gatatacgtt tgcatcgtct
     3181 atatttttgc caatacgata aggcccattg tgaataactt cattcggaaa cggattcgta
     3241 tgcaatgtga acgagtgatc ggttgtagaa aaattcgcat ggtcacgtag gaaatacgat
     3301 gtaatatccc atagccactg ttcatatttc gagagatatt cggagctttc ttcaagattg
     3361 atgcggagtt tttcgtgaac ttcctcatcg aagttttcca gcagtttctg ttttgtcttt
     3421 gacatttctc cggaaatctg ttcttccagt tccttttgaa gaaagtcaaa cgagagttgg
     3481 atttcgtccg gcgtgcggca gtcctgataa atttttgcaa tacgcttttc gaaatccaca
     3541 ccactttcaa tactacccag cacttcgtcg cttgcgccga acacgccact aaagagcttg
     3601 aacttttgat ccaagagatc atagacgcgc tgatcggcag cattggcttt atttagaaag
     3661 ttcacgacaa ccacatcgta cttctgacca taacggtgac atcggccgat gcgttgctcg
     3721 atgcgctgag ggttccaggg cagatcataa ttgacaacca acgagcagaa ctgcaggttg
     3781 atgccttccg cggctgcttc cgtagcaatc ataatggttg cggtatcacg gaagtattct
     3841 acgatcgcgg ctcttttatc cgccgtctct gagccggtaa tacgatccga attcttattt
     3901 cgctcaatcc actgacgata aatatcgcgt gactccgggt ctgtatttga accattgaag
     3961 agcacgatct ggccggcata gccattttgc tcgagaatct gcttgaggta gttctgagta
     4021 cggatgcttt ctgtaaagac tatggccttt ctttgagcac ctagtcctac agcctcttca
     4081 aagcctcgct tcagcgccgt gatcagctta tcgccctttg tatttatctt gatagattta
     4141 gcgagtgtgt gaaattcctg aagcgtggcc atttctcttt tcatgcctgc gatctcttcc
     4201 ggcgtaaact tgggtggcgc ggcaacggac tcctcttctt catcgctctg ccattcgtct
     4261 tcaaattctt tgatggcttc gaaatctgta aattcctgtt ccccgatagg cacgggcagt
     4321 ccattctgct tcgtggcttg atcaagcttt gatgccaggg cttcaagtgt acccgaaata
     4381 gcaaacgtgg aggacgccaa caatcgccgg agaatcagtg tcatcaatga tcgctggctt
     4441 tttggcaaag catataaatc tgcgcgttgg aggtactccg acacgagctc gtataatttc
     4501 tgctcttcgg cggacggata gaactcctcc acgatagctt ttcgatttgt gaatttgata
     4561 tattcgagaa cctgcctccg cagtgtccgg tggcaaatgg gacggaggcg ttgtttcaat
     4621 tcaagataca gttcgtcgct ttcttcactt cctaatcgac tgtattgcat tttgaagctt
     4681 ttcagatctc caaaatggag atcgtctatc acgctgacca agccgtataa ctccaaaatt
     4741 gaattctgaa gtggtgtggc tgttaacaaa atctttcgcg tgtcggcaac ggcgttcttg
     4801 attgcgttgg caattttgtt agtaggtttg tacacattgc ggaggcggtg cgcttcgtca
     4861 atgacgacta aatcccaagg tatcgttttg atgtaaggtt cttgtttgcg ggcaaattga
     4921 tatgagcaaa ttacaatgtc cttttgatcg aatggattca agtttccatt tcgaattgca
     4981 ttgttgaagc ttgcattctc gataataact gttggaagaa aaaacttatc gagaagttct
     5041 tggctccatt gcttgcgaag gtttgccgga actatgatca ggattttacg atgtcgttcg
     5101 gcccatttct gcgatatcac taggcccgcc tcgatggtct tgcctagacc aacttcatcg
     5161 gcaagaatag cacctttaga cagtggggat cgaaaagcaa acagagccgc ttcgacctga
     5221 tgggggttta ggtcgacctg agcatccacc aacgcggttg ccagcttttg aatgctgtct
     5281 gaggcacttc gtcgggttag ttcatacgcg aagtatttgg catgatatgg tgtcaagaac
     5341 cttccccttt gtcatcttcc cgttcaatcg gtgcatcgtt gatgcctgcc agtagttctt
     5401 gtaatatcgt ttgagcttcc tcgagagagc tgatcatatc ccggatcatg agtgttgcca
     5461 gcttactggc ttgctggtct tgaatggctt tttccaagat acccgtgact cgcgtggcca
     5521 gaggaacaga tgaattcata atcgaattca tgtgacgacc gagcaggcgt gcgccgatct
     5581 tgaatgtgtc ggaatcgttt tccagcgtct tcaataagtc atgatggcgc gtgaagatgt
     5641 tcatttgctc a
//
LOCUS       DAOQGF010000169         5591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_7323_length_5591_cov_5.310874, whole genome shotgun sequence.
ACCESSION   DAOQGF010000169 DAOQGF010000000
VERSION     DAOQGF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7323_length_5591_cov_5.310874"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK937_14890"
     CDS             <1..279
                     /locus_tag="PK937_14890"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNH33910.1"
                     /translation="PLDVMKLCAAAIGLTHTVVTRGNTNALSDGGVGALMLQAACRGA
                     YYNVMINLGGLKDAAFVSAIQSEAQITMKTVDDAALAISQHVGNQFGV"
     gene            276..425
                     /locus_tag="PK937_14895"
     CDS             276..425
                     /locus_tag="PK937_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HNH33911.1"
                     /translation="MNLDTILFFIVVVGLLSFTFLIYIGRKRKKSIGERKERIKNQWR
                     NRLEE"
     gene            428..2188
                     /locus_tag="PK937_14900"
     CDS             428..2188
                     /locus_tag="PK937_14900"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNH33912.1"
                     /translation="MEPMDKPLDKQSLIKGEVQDDVDRKLLELSALFEISQLLNSSLN
                     LTSIVDNILLSPMGRMMINKGLFLLHRGDHQYEIFTLKGLSRDLIGKKISITEIPQKS
                     FLLDEVSDAPYPWLSFFTQYDMKLIVPIVLRNDLLGLVAFGKKLIGGPYIESEIEFLS
                     SIANIAATSIQNGIVFEELRSVNARLDKKIQELHTLFDIGKELNATLDAQKVVKLLSY
                     GLMGEMMVNRFAILVRRHDGVEYAEKRGLARLQLDDTQTSLVFNYSQPAVVTDDEHSE
                     LQHALQNQDIIAIVPMRSQDRTKGVLLLGPKISGKGFTKEELDFVFTLGNQAMISLEN
                     AWLFEDSLERKRLEEELELARKIQMGLLPKDFPKLNNFEVYGLNISSKQVGGDYFDVL
                     KINETQYIIAIADVSGKGVPASLLMSNVQASLLALSTEVSDFSKLVYKVNNIIYANTD
                     SEKFITFFGGVLDIEKRTFTYVNAGHNPPYWRKTDGTLHTLDKGGLLLGMLPDMPYEQ
                     ETITLSPGEGIVMFTDGVTEAMNEKLDQYDESRLEALIKTHPKITAKECTEKIAAEVK
                     TFAGAAPQSDDITVVILHAI"
     gene            2259..4604
                     /locus_tag="PK937_14905"
     CDS             2259..4604
                     /locus_tag="PK937_14905"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH33913.1"
                     /translation="MNKMLVKNLFFLALFVTVAVVNSQETGVITGVVIDAKTKEYLAG
                     VNVQILGTTRGTVADINGQYRLDKLADGTYRLQFSLIGYAPLIKTDVIVTGNKPFRLN
                     VAMEAESIEGDEVIVTAGYFEEQNQTKTSTANLSREEIRRFPGGFEDVVQTVATLPGV
                     AVNGGGGRNDLLVRGGGPSENLYIVNGLEIPNINHFGTQGNNGGSLSFINLDFVDNVD
                     FSTGGFSARYGDKMSSVTQLTLRSGRDDRIGGKGLVSATQFGVNTEGPLGKRGNFIMS
                     ARRSYLDFIFKAAGFAFVPRYTDFNMIGQYEPSPRDKFTFLALTALDDVSKNNEKRKD
                     RIFNSGLLDNEQFIQVVGAGYQHNLEKGFWQVTANVNFSGYRFSQKDTTLREYYNSRA
                     NEVETNASVQRYFVINKSMGILTGLSMKFVNNRNETVFGDTIVDRNGNRIPADSLGLA
                     GLDETRASAYKYGGFTEMDWVVSPRLNANAGLRVDRYSALEKKTYVAPRLAFKYKISD
                     KSNLKLSGGTYYQSPSYVWLVNPANKKLKALRNDMVVAGADRLLRPDVKLSIETYYKK
                     YSHLPTGTQKGINDYIVITSTGTGFGGREDNFQSFGYATLVSRATGKAFGVEMLLQKK
                     FSDIPCYGQVSITVGKSTVVAGNGKTYPNPFDQRFILNISGGYKLNTKWEFSGKFRYF
                     TGAPYTPVYRPQDNPVQPGVVQNLPDEYLSKRLGASHHLDLRADRYFNYEHWRLIIFM
                     DVQNVYNYKVKTPPSYDFAEDKVTRGDRIGILPTIGISAEF"
     gene            4621..>5591
                     /locus_tag="PK937_14910"
     CDS             4621..>5591
                     /locus_tag="PK937_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33914.1"
                     /translation="MPKILICVIALNLTGCGFFITPRSSDQVSIREENLVAHLTYLAS
                     DELEGREAGTRGEKLAAGYIASQLRQNGVKPFALARTADGRDSLSYFQEFSSTKIAIG
                     KHSSVIFYDSASGNSFTMPYAETFSNFHEYVDNCNVSAPLVFAGFGITAPEYGYDDYA
                     GLDVRGKVVLVLDGEPTSEDDQFFDGKIVSSYSSALFYKRHRAKELGAAALFVLSYQA
                     LLEKWDEYVDFFGSSRMIFDPSLLPKSEGERVPFYYCHTVFAKRALASAETGFEQIQS
                     KAQSGAMVPRFTVKNIRCDVRIERTVTQVKAMNIFHGKTRHHRTGLCL"
BASE COUNT         1514 a         1161 c         1416 g         1500 t
ORIGIN      
        1 cctctggatg ttatgaagtt gtgcgcagca gcaatcgggt tgacccatac ggtcgttacg
       61 cgcggcaata ctaacgccct cagcgacggc ggcgtcggtg cattgatgct gcaggccgct
      121 tgccgtggag cttattataa tgtgatgatc aatttgggcg ggctcaagga cgcggcattt
      181 gtatcggcga tccaatcgga agcgcaaatc accatgaaaa ccgtggatga tgcggcgctt
      241 gcgatcagtc aacacgtagg caatcagttc ggcgtatgaa tttagatacg atactttttt
      301 ttattgtcgt tgtcggattg ttgtcgttta cgtttttgat ttatatcggt cgtaaaagaa
      361 aaaaaagtat aggcgagcgt aaagaacgca tcaaaaacca atggcgtaac cgccttgagg
      421 aatgagtatg gaaccgatgg ataagccgct ggataaacaa tccctcatca aaggcgaggt
      481 gcaggatgac gtggatcgca aactgctgga actgtctgcg ctttttgaaa taagtcagtt
      541 gctcaattct tccctcaatc tgacttcgat cgtagataat atcttgctct caccgatggg
      601 ccgcatgatg atcaataaag gtcttttttt gctgcatcgc ggtgatcatc agtatgaaat
      661 atttaccctc aaaggtttat cccgcgatct gatcggaaaa aaaataagca tcaccgagat
      721 accgcaaaaa tcctttctcc tcgatgaggt ttccgacgcg ccgtatccgt ggctttcgtt
      781 ttttacgcag tacgacatga agctgatcgt gccgatcgtt ttgcgcaacg atctgctcgg
      841 gttggtcgct ttcggaaaaa aattgatcgg cggtccgtac atcgaaagcg aaatcgaatt
      901 tttatcgtcc atcgctaata ttgccgccac cagcattcaa aacggtattg tgttcgaaga
      961 gttacgctcg gtcaatgcgc gcctggataa aaaaatacaa gaactgcata ctctttttga
     1021 tatcggcaag gaactcaacg ccacgttaga tgcgcagaag gtcgtcaaat tgctttccta
     1081 cgggcttatg ggtgaaatga tggttaatcg tttcgcgatt ttggtgcgtc gccatgacgg
     1141 tgtcgaatat gccgaaaaac ggggccttgc ccgattgcag ctcgatgaca cccagacgtc
     1201 gctggtattc aattattcgc aaccggcggt ggtgacggat gacgagcaca gcgaactgca
     1261 gcacgctctt caaaatcagg atattatcgc aattgttccg atgcgaagcc aggatcgcac
     1321 aaaaggtgtt ctgcttctcg gtcctaaaat ttccggaaaa ggatttacta aagaagaatt
     1381 agattttgtt tttacgctgg gtaatcaggc gatgatttcg cttgaaaacg catggctttt
     1441 cgaagattcg cttgagcgta aacgattgga agaagaactt gagcttgcac gcaaaattca
     1501 gatgggtttg ttacctaaag attttccaaa actgaacaat tttgaagtgt acggtttgaa
     1561 tatttcatcc aaacaagtcg gcggcgatta ctttgatgtg ctcaaaatca atgaaacgca
     1621 atatatcata gcgatcgcgg atgtgagcgg taaaggcgta ccggcttcgc ttttgatgag
     1681 caatgttcaa gccagtttgc tggcgctcag taccgaggtc agtgatttta gcaagcttgt
     1741 ctataaggta aataatatca tttacgcgaa taccgattct gaaaaattta tcacgttttt
     1801 tggcggtgta ctggatatcg aaaaacgtac gtttacatat gtcaatgcag ggcacaatcc
     1861 gccgtattgg cgaaaaacgg atgggacgtt acatacactg gataaaggcg gtttactttt
     1921 gggtatgttg cctgacatgc cgtatgaaca agaaacgatc acgctttcac ccggtgaagg
     1981 catcgtcatg tttaccgacg gcgtgacgga ggcgatgaac gaaaagctgg atcaatatga
     2041 cgaatcgcgc cttgaagcat tgatcaaaac gcatccgaag ataacagcca aagaatgtac
     2101 ggaaaaaatt gccgctgaag tcaaaacctt tgccggtgcg gcgccgcagt cggacgatat
     2161 caccgttgtt atcctccatg ctatttaaga gcctctcttt gttttggaaa catagcggcg
     2221 tgatgtaaaa catccgccgt ttttgatatg aaagtgatat gaataaaatg ttagtaaaga
     2281 atttattttt tttagcgttg tttgttaccg tcgctgtagt caatagccaa gagaccggtg
     2341 tgatcacggg cgtggtcatt gacgcgaaaa ccaaagagta tcttgccgga gtgaatgtgc
     2401 agatactggg cacaacgcgc ggaacggtag ccgatatcaa cgggcagtac cgattggata
     2461 aattggccga cgggacatac cgcctgcaat tttcactgat cgggtatgcg ccgctcatca
     2521 agaccgatgt cattgtcacg gggaacaagc catttcggct caatgttgca atggaagcgg
     2581 aaagtatcga aggcgacgaa gtgatcgtaa cggcgggata ttttgaagag caaaatcaaa
     2641 ctaagacgag cacggctaat ctatcccgtg aagaaatacg gcgatttccc ggcggttttg
     2701 aagacgtcgt gcaaaccgtg gctacactgc ccggtgtggc ggtcaatggc ggcggtggac
     2761 gcaatgattt actggtgcgc ggcggcggac cgtcagaaaa tttgtatatc gtcaacggat
     2821 tggaaattcc caatatcaat cactttggca cgcagggaaa caatggcggt tcgctgagct
     2881 tcattaatct ggatttcgtg gataatgttg atttttcgac aggtggtttt tcggcgcggt
     2941 acggtgacaa gatgtcatcc gtcacacaac ttacattgcg aagcggacgc gatgatcgta
     3001 tcggaggtaa agggctggtt tcggcgacgc aatttggcgt caacaccgaa ggaccattgg
     3061 gaaaaagagg aaactttatc atgtccgccc gacggagtta tttggatttt attttcaaag
     3121 ccgccggttt tgcttttgtg ccgaggtaca cggattttaa tatgatcgga caatacgaac
     3181 catcgccgcg tgataaattc acttttctcg cattgacggc gcttgatgat gtgagtaaaa
     3241 ataatgaaaa acggaaagat cgtattttta attccgggct tcttgataat gaacaattca
     3301 tacaagttgt tggtgccggt taccagcaca accttgaaaa aggattttgg caggttacgg
     3361 ccaatgtcaa tttttcagga tatcgttttt cacaaaaaga tacgacgcta cgcgaatatt
     3421 ataacagccg tgccaatgaa gtggaaacga atgcttccgt tcagcggtat tttgtgatta
     3481 ataaatccat gggtattctg accggattga gtatgaaatt cgtcaataat cgcaatgaaa
     3541 ccgtatttgg tgatacgatc gtggatcgca atggtaatcg tattcccgcg gattcgctgg
     3601 gcctggcggg attggatgaa acacgtgcgt cagcgtataa atacggcgga tttacggaaa
     3661 tggattgggt cgtgtcgccg cgcctcaatg ccaatgcagg tttacgcgta gatcggtact
     3721 cggctttgga aaaaaaaacg tatgttgctc cgcgtttggc attcaaatat aaaatatcgg
     3781 acaaatccaa tcttaagttg agcggcggaa cctattatca atcgccatcg tacgtatggt
     3841 tagtcaatcc ggctaataaa aaactcaaag cgttgcgcaa cgacatggtc gtagcgggtg
     3901 cggaccgttt actgcgtccg gatgtgaaac tctcaataga aacgtattac aaaaagtaca
     3961 gtcatctgcc gaccggaacg caaaaaggta tcaatgatta tatcgtcatt accagcaccg
     4021 gcaccgggtt tggaggacgt gaagataatt ttcaatcctt cggatatgcg acgttggttt
     4081 cgcgtgctac cggaaaagcg tttggcgtcg aaatgttact ccaaaagaaa ttttctgaca
     4141 taccatgtta cggtcaggtc agtattacgg tgggtaaaag tacagtggta gcgggcaacg
     4201 gtaaaaccta tcccaatccg ttcgatcagc gctttatttt aaatatttcc ggcggttata
     4261 aactgaatac caaatgggaa ttcagcggga agtttcgcta ttttaccggc gctccgtata
     4321 caccggttta tcgtcctcag gataatcccg tgcagccggg tgtcgttcaa aatcttccgg
     4381 atgagtacct ttccaaacgc ctgggagcga gccatcatct ggacttacgc gcggatcggt
     4441 attttaatta tgaacattgg cggcttatta tttttatgga tgtgcaaaat gtatataatt
     4501 acaaagtcaa aacgccgccg tcatatgatt ttgcagagga taaagtgacc cgtggtgatc
     4561 gcataggaat actgcccacg atcggtatca gtgctgaatt ttaatctaac ggagaaggat
     4621 atgccgaaaa tattgatttg cgtaatcgcc ctgaacctaa ccggttgcgg tttttttatc
     4681 acgccccgct cgtcagatca ggtatccatt cgcgaggaaa atttagtggc tcatttgacg
     4741 tatctcgcat cggacgaatt agaaggacgg gaggcgggta cgcgcggaga aaaattagct
     4801 gccggttata tcgcttcgca gttaaggcaa aacggtgtta aaccttttgc gctagcccga
     4861 acggcagacg gccgagacag cctgtcgtat tttcaggaat tttcttcgac taaaattgcg
     4921 atcggcaaac attcatccgt gattttttat gacagcgcaa gcgggaattc gttcaccatg
     4981 ccttatgctg aaacatttag taattttcat gaatatgtag ataattgtaa cgtttctgcg
     5041 ccgctcgttt ttgccgggtt tggtatcacg gcgccggaat acggatacga tgattatgca
     5101 ggactggatg tacgcggtaa agtcgtatta gtgcttgatg gcgaaccgac atcggaggat
     5161 gatcagtttt tcgacgggaa aatagtaagt tcgtatagca gcgctttatt ttataaaagg
     5221 catcgggcca aggaattggg tgcagcggcg ttgtttgtat tatcatatca agcgcttttg
     5281 gaaaaatggg atgagtatgt ggatttcttc ggatccagcc gtatgatttt tgatccatcg
     5341 cttttaccga aatcggaggg cgaacgggtt cctttttatt actgtcatac ggtatttgcc
     5401 aaacgtgcat tggcatccgc cgagacgggt tttgagcaaa ttcaatctaa ggcacagtcc
     5461 ggtgcgatgg tgccgcgttt taccgtgaaa aatatacgtt gcgatgtgcg tatcgaacgc
     5521 acggttacac aagtgaaagc gatgaatatt tttcacggta aaacgcggca ccatcgcacc
     5581 ggactgtgcc t
//
LOCUS       DAOQGF010000170         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_7494_length_5501_cov_7.587771, whole genome shotgun sequence.
ACCESSION   DAOQGF010000170 DAOQGF010000000
VERSION     DAOQGF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_5501_cov_7.587771"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5501
                     /locus_tag="PK937_14915"
     CDS             <1..>5501
                     /locus_tag="PK937_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNH33915.1"
                     /translation="SLRGDESGLAGLWHFDESSGTTTFDATAGGNNGTLINGPTFTAS
                     GAMPLVVTSILDNGAGTLRSAINFANANPGADTIRFATNIDGATISLASALPMLTDDS
                     TFINADINNDSLPNIFLDGQGTTPNGFNVASTAIRATIQGFHIDGFTSNGITSDAPFT
                     AIQYNHFGLISANAQGITLTAEDNKVFRNVIAGGGTGIYGGSDATVDTTMILDNLIGT
                     NASGTVALRPGNGIYVTYRITQKGWTIKNNVINGNTNAIQLNSAFSFPLNGFTIIGNK
                     IGTDKDGVNALGGNTGILIGQNSRLHHIGDGTVPGGNLISGNSSFGIQISNTDSIWIL
                     GNYIGTDVTGNASLGNNAGVSVSTSNNIWIGDGTIGGRNVISDNTTFGVGVSGGDSVR
                     ITGNYIGLGVNGNTALGNQSYGVSLANDEYTIVRDNVVASNNSEGMYITGGMYRIVDN
                     NIIGLNAAGNLVRPNSAGIFMSSSKYNKIGESLGNTISGNTNDGLIFSSADSNEIYKN
                     VIGLGTAGVATFANGGTGIILQQSSANYIGNGAASGRNIISSNAGHGIRIIATAAKAE
                     NNLIRNNYIGVGADGSLIRGNGQNGISLYSIPSFNTTGNYFNANVIAHNGFYGVEADG
                     ASDADSNQLDQNILFANTLGGVNYVSGSQGGVASPRIEAMFDDSTVAIQAAPGAYIQV
                     FADAGSQGEIPLITGFADASGGYLGDVRLALRNYFGYNITATQDSAGNTSGFAVNTWP
                     FGLTDPTLVANNNNSGTGSLRFAINYANSHAGPDTIRFDVPMVGQTIFPATMLPDITD
                     DSTVIDGDIDMNGLPDITLNGAPFNVPIGLTINSNYNEIRNLNITGFGSMASGIDING
                     GAYNLIVGCRIYNNNDGVNVFNGAHHNRIGDGTIAGRNYIYNNTDAFTGFGILIEGFG
                     TDSNIVYGNFIGTADGLTESGNSSDGVMVRNQARYNRIGGVGGSYNVISGNGRNGIRI
                     ESDSNTVTGNFIGLDSIGTGALGNQGYGIHLLRAHANTIGGTAVGSGNVIVDNSQANL
                     NGAGVYLDSATYNEILGNYLGVDVYGNSSIANQSGGGGGSSITLDNQSNHNIIGDGTA
                     GGRNVMGAGGSGADMASHVLITNNSNFNLIAGNYIGVGSDGATALGIGSGVYISGSIK
                     NQIGLPAPAEANIISGNPDGGIVLSDADSTLVLQNYIGVNASGNAAVGNSFGVRLNNG
                     TTHANIGNATVSGRNIISGNSNFGISIEDVGTDSNLVRSNLIGLDLAGTTAVPNSNAG
                     ISIQDGVDNEIGDGSVGGRNVISGNSGHGIYNFSRTRIAGNFVGLDISGNDAIGNTGY
                     GIYTVSDSTQISGNVISGNSFTGIQVVYGRKAQIQGNAIGLDATGTIQRANGTAGVVL
                     FNHSDALVESNTITGNTGDGIRIEELSFGNTIFSNNIGMKDGPIGNSQNGIVLIDNGS
                     SYPSNNIIDSNYIAYNGNFGITIQGTQTDSNAAARNYIYKNIGGSIDISGGAQQGINP
                     PTITLGTYGGTVAGTANSNALVYVYADSADQGEYFLDTVRADGSGNWTVSVTPVSGTR
                     YTAIQEYNAQTSEFSAPFIDPLLVINTNDAGAGSLRDAVTYAESHSGADTIRFAVALD
                     GQSITLLNPIYFNGDSTTVTGDIDSDFIPDITLVRGASTNWGFAVNGNHNVISHLNFQ
                     QFNTAGILINGHHNHIRGNTIGTNLDGSDNTGTGNTWGIQLVNSAKYNTIGDTVNAGR
                     NIIAGNTGYGIVFQNNSDSNAVIGNYIGLNAAGDNAFGNGTAGIVIDAGPKNNTIGNG
                     LANGRNIISGNNYAVFITGAGANYNRIIGNFIGTDPLGTTAIPNPSGGI"
BASE COUNT         1470 a         1138 c         1375 g         1518 t
ORIGIN      
        1 atcgctccga ggtgatgagt caggtcttgc cgggctttgg cactttgatg aatctagtgg
       61 tacgacgaca ttcgatgcta cggctggggg taacaacggt acgctgatca atggccctac
      121 atttaccgcg tcgggcgcta tgcctttggt agttacttcg attcttgaca atggtgcggg
      181 tacattacga tcggctatta attttgccaa tgctaatccc ggcgctgata cgattcgttt
      241 tgcaactaat attgatggag ctacgatctc acttgcatcg gcacttccaa tgttgacaga
      301 tgactctacg tttatcaatg cggacatcaa taacgacagt ttacctaata tatttttgga
      361 cggacaaggt actacgccta acggttttaa cgtagcctcg acggcgattc gtgccacgat
      421 acaagggttt catattgacg gatttacatc caatggcatt acgagcgacg cgccgtttac
      481 ggcgattcag tataaccatt tcgggcttat cagcgctaat gcgcaaggga tcacgttgac
      541 ggcggaagat aacaaagtgt tccgtaatgt tatcgcgggc ggtggaacag gtatttacgg
      601 tggttccgat gctaccgtcg atactacgat gattttagat aatctgatcg gtacgaatgc
      661 atcgggtact gttgcattgc gtccaggcaa cggtatttat gtcacttatc gtattactca
      721 aaaaggctgg acgatcaaaa acaatgtcat caatggtaat acgaatgcga tacaacttaa
      781 tagcgcattt tctttcccgc taaatggttt tacgatcatt gggaataaaa tcggtaccga
      841 caaagacgga gttaacgcac tgggcggaaa tacgggtatc cttatcggtc aaaattcaag
      901 actacatcat atcggcgatg gtacagtgcc cggtggaaat ctgatttccg gtaattcgag
      961 ttttggtatt cagataagca atacggattc aatatggatt ttaggtaatt atataggaac
     1021 tgatgtgacc ggtaatgcgt cgttgggaaa caacgcgggc gtatctgtgt ccacatccaa
     1081 taatatatgg ataggcgatg gcacgattgg cgggcgtaat gtgatttccg ataatactac
     1141 gttcggtgtt ggagttagcg gcggcgacag tgttcgtatt accggaaact atatcggcct
     1201 cggcgtcaat gggaacacgg cgcttgggaa tcaaagctac ggtgtatcgt tggcgaacga
     1261 cgaatatacc atcgtacgag acaacgtggt ggcttcaaac aacagcgagg gcatgtatat
     1321 taccggtggt atgtaccgta ttgtggacaa caatatcatc ggtttgaatg cggctggaaa
     1381 tcttgtgcgt cccaacagcg ccggcatttt tatgtcttct tcgaagtata ataaaatagg
     1441 cgagtcgctg ggtaatacca tttcgggaaa taccaacgac ggcttgattt tttcatctgc
     1501 ggattccaat gaaatatata agaatgtcat tggtctcgga acggcaggag tggctacgtt
     1561 tgccaatgga ggtaccggga taatactcca gcagtccagc gctaattaca taggaaacgg
     1621 agcggcctct ggtcgtaata ttatttcttc gaacgcgggc catggtatac gtatcattgc
     1681 tacggccgcc aaggcagaaa ataatctgat tcgcaataat tacatcggag tcggagctga
     1741 cggatccctg atccgaggta acggacaaaa tggtatcagt ctttattcga ttccttcttt
     1801 taacaccacc ggtaattatt ttaacgcgaa tgtcatcgcg cacaacggtt tttatggcgt
     1861 cgaagctgac ggagccagtg atgcggatag taatcaattg gatcagaata ttctatttgc
     1921 caatactttg ggtggtgtta attacgtctc tggatcgcag ggtggcgttg catcgccgcg
     1981 tattgaagca atgtttgacg attcgacagt tgcaatacaa gcggccccgg gtgcatacat
     2041 tcaagttttt gccgatgccg gatctcaagg tgaaatccct ttgattacag ggtttgccga
     2101 tgcatcgggc ggttatcttg gtgatgttcg cctggcctta cgtaattatt ttggctacaa
     2161 cataacggct acacaagata gcgccggtaa tacgtccgga tttgccgtta atacatggcc
     2221 gtttggctta acggatccga cacttgttgc caataacaat aattccggta cgggaagttt
     2281 gcgttttgca atcaactatg ccaattcgca tgccggacct gataccattc gttttgatgt
     2341 accgatggtg ggacaaacga tttttcctgc cacaatgctg cccgatatca cagatgattc
     2401 gacagtgatt gacggtgata ttgatatgaa tggtttaccg gatatcacat taaacggggc
     2461 cccgtttaat gtacctatcg gtttgacgat taattcgaat tacaacgaaa ttcgtaattt
     2521 gaatattacc ggttttggtt cgatggcgag cggtattgat attaacggag gcgcatacaa
     2581 tctcatcgtc ggttgccgaa tatataataa taatgatggt gtcaatgttt tcaatggtgc
     2641 gcaccataac cgtatcggtg acggtaccat tgcaggtcga aattatattt ataacaatac
     2701 ggatgctttt acgggattcg gtattctgat cgaagggttt ggtacggatt cgaatatcgt
     2761 ctatggtaat tttatcggta cggccgacgg tcttacagaa tcaggcaaca gttcggacgg
     2821 tgtgatggta cgcaatcaag cacgatacaa tcgtattggc ggtgtcgggg gatcatataa
     2881 cgttatttca ggaaacggac gcaatggtat tcgtatagaa tccgacagca ataccgtgac
     2941 cggtaacttt atcggtttgg attctatcgg aaccggggcg ctcggcaatc aaggatacgg
     3001 aattcactta ttacgtgctc acgccaacac gatcggcggt acagcggttg gctccggaaa
     3061 tgtgattgtc gataattcgc aagctaatct taatggcgca ggcgtttatc tcgatagtgc
     3121 aacttataat gaaatattgg gcaattatct cggcgtggat gtgtatggca attcatccat
     3181 agccaaccaa tccggcggcg gtggcggttc aagtattacg ctggataatc aatccaatca
     3241 taatatcatc ggagacggta cagcaggcgg acgcaatgtg atgggtgccg gtggtagcgg
     3301 cgctgatatg gcttcgcatg tacttattac aaacaactcc aattttaatc tcatcgccgg
     3361 taactatatc ggtgtcggtt cagacggcgc cacggcatta ggtattggaa gcggtgttta
     3421 cataagcgga agtataaaaa atcaaattgg tttgccggct ccggctgagg ccaatatcat
     3481 atcgggtaac cctgacggtg gtatcgtgtt gagcgacgcc gattctacac tagtattgca
     3541 aaactatatt ggtgtcaacg cgtctggaaa cgcagccgta ggcaacagct ttggtgtccg
     3601 gttaaacaac ggaacaacgc acgctaatat cgggaacgca acggtctcag gtagaaacat
     3661 tatttccggc aatagcaatt tcggtatatc catagaagat gtcggcacgg atagtaactt
     3721 agtacgcagc aatttgatcg ggcttgatct cgcaggtacg acagccgttc cgaattctaa
     3781 cgccggtatt tccattcagg atggagtgga taatgaaata ggtgacggtt ctgtcggagg
     3841 gcgcaatgtt atttccggta attcaggcca tggtatttat aattttagtc gaacacgtat
     3901 cgcaggcaat ttcgttggtc ttgacatcag tggcaatgat gctatcggaa atacaggata
     3961 tggtatttat actgtatcgg acagtacgca aatatccggt aatgttattt caggaaattc
     4021 atttaccgga attcaggtgg tgtatggtcg taaagcacaa attcaaggga atgcaatcgg
     4081 tcttgacgcg acaggcacga ttcaacgtgc caatggtaca gccggtgttg tactttttaa
     4141 tcattccgat gctttagtcg aaagtaatac aataaccgga aataccggtg atggcattcg
     4201 tatagaagag ttgtcttttg gtaatactat ctttagtaat aatataggta tgaaagatgg
     4261 tcccataggc aattcacaaa acggtatcgt gcttattgat aatgggtctt cttatccgtc
     4321 taataatatt attgattcta attatatcgc atacaacgga aacttcggaa ttactattca
     4381 aggtacacaa accgacagca atgcagcggc acgcaattac atctataaaa acatcggcgg
     4441 cagtattgac atcagcggtg gtgcgcagca gggtataaat cctcctacga ttacattggg
     4501 gacgtatggc ggaacggttg ccggtacggc aaacagtaat gcgctagtgt acgtttacgc
     4561 cgattctgcc gatcaaggtg aatatttctt agatacagtt cgtgcggatg gctccggtaa
     4621 ctggaccgtc agcgtaacac ctgtcagtgg cacacgctat accgccattc aggaatataa
     4681 tgcgcagacg tcggaatttt ccgcgccgtt tatcgatccg ctattagtca tcaataccaa
     4741 cgatgccggt gccggttcac tgcgtgatgc ggtgacgtat gcggagtccc atagcggagc
     4801 ggatacgata cgatttgcag ttgcgcttga tggtcagtct atcacgttgt taaatccgat
     4861 atattttaac ggtgattcaa ccacggtcac cggtgatata gattctgatt ttataccgga
     4921 tatcaccttg gtgcgcggcg cttcaacgaa ctggggcttt gccgtcaatg gtaatcataa
     4981 tgtgatcagc catcttaatt tccagcagtt taacacagcc ggtatcctta ttaacggcca
     5041 ccataatcat attcgcggta atacgatcgg tacgaatctg gatggctcgg ataataccgg
     5101 tacgggaaac acctggggta tccagcttgt aaattcagcg aagtataata ccatcggtga
     5161 tacggttaat gcaggccgta atattattgc cggtaatacc ggatatggta tcgtattcca
     5221 gaataattcg gacagtaacg cggttatcgg aaactatatc ggtctcaatg cggcaggcga
     5281 taatgccttt ggtaatggaa cagcgggtat cgtgatagat gcaggaccga agaataacac
     5341 gatcggtaat ggtttagcta atggccgtaa cattatttca ggtaataatt acgcggtatt
     5401 catcacaggt gccggcgcta actacaatcg cattatcggt aattttatcg gaaccgatcc
     5461 gcttggaact acggctattc ccaatccttc cggtggtatc a
//
LOCUS       DAOQGF010000171         5428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_7664_length_5428_cov_6.294435, whole genome shotgun sequence.
ACCESSION   DAOQGF010000171 DAOQGF010000000
VERSION     DAOQGF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_5428_cov_6.294435"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK937_14920"
     CDS             <1..268
                     /locus_tag="PK937_14920"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNH33916.1"
                     /translation="VLVRVTVKGIKGGNPKEIQYQIVDYGDASNNITAMMRLTSYPIS
                     VIAQMMTEGKITEKGAVPQELAVPTRSFIEALRARNIDIQITES"
     gene            633..977
                     /locus_tag="PK937_14925"
     CDS             633..977
                     /locus_tag="PK937_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33917.1"
                     /translation="MRRTTSAARKTKQNPVLMDLENYILKLGLNSEPTSANVVAKRLE
                     LKESPKNIQMIKQALEKMVREKKVYRCGSRYAIDYQGGFNLMKTFCIKVAEENYKINN
                     FVFEGVAYARGA"
     gene            1111..2088
                     /locus_tag="PK937_14930"
     CDS             1111..2088
                     /locus_tag="PK937_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH33918.1"
                     /translation="MQELIRVESLCKSYPVGSTLWGRATWSQVVEDVSFIVRTGETLG
                     VVGESGSGKSTIGRCLLKLTDPTSGRIFFDGEEITALDNKAMRPFRRRMQIVFQDPFS
                     SLNPRQTVDAMFREIFTVHATASGTEATDKIEELLKTVGLDPSQRFCFPHEFSGGQRQ
                     RIGIARALSVRPQFIVCDEPVSALDVSVQAQIIELLMTLQETRNLSYLFIGHDLAVVR
                     HIADRIAVLYRGQMMEMGPSDVLFDQPLHPYTQALIASVPEPDPSKPVKPVAVKGDIA
                     DSSRPSKGCLFQARCPWVEAVCRDETPRWRSCGQERFVRCHKVTGDNPH"
     gene            2160..2510
                     /locus_tag="PK937_14935"
     CDS             2160..2510
                     /locus_tag="PK937_14935"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNH33919.1"
                     /translation="MEDILKRLRNDTPGVSAVALVSSDGLVISSMLPDTIEAERVAAI
                     SAALLNQGESSSEDAMIGEMAQVTIRAKEGFIIITRAAKETLLTVFTTTQAKLGLVLF
                     DISVAIKELQPHIH"
     gene            2517..2924
                     /locus_tag="PK937_14940"
     CDS             2517..2924
                     /locus_tag="PK937_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33920.1"
                     /translation="MIASHDQYFGEIIRTVEAIAPQTLVAVISWEGLVLRSNVSDEHV
                     EGQFGALASVFLDQGKRMFTIPFDGKKPQPDTVQTMVTVGTKHVIIMTQLLPGILLAV
                     SSGDKSKINTFLTCSLEEVDKLHAMIAKKEIIF"
     gene            2939..4294
                     /locus_tag="PK937_14945"
     CDS             2939..4294
                     /locus_tag="PK937_14945"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HNH33921.1"
                     /translation="MKRIYNNILEVIGQTPVVKLNRIGAQLQCNLYAKCEFMNPGGSI
                     KDRIGRAMVEAAEREGRIKPGDTLIEPTSGNTGIGIALAGAIKGYRVIITMPEKMSRE
                     KQVILEALGAEIIRTPTEAASEDPESHISVAQRLQRELPNAHILDQYSNPNNPKIHYD
                     TTGQEILDALDGKVDMVVVAAGTGGSITGIAKRMKDANPQCIVVGADPVGSILGGGTH
                     VGTYKVEGIGYDFIPKVLDRDCVDEWIKTEDQSSFLLARRLIHEEGLLVGGSSGSSLF
                     AALQAAQRLKPGQNCVVVLSDGVRNYMTKFVDDKWMRDNGFFENKSIQGTVKDILAKN
                     PARPVLICADDMESVRQTISKMREKGVSQLPVTSGGALVGIVSESDIMDYLASGAGTQ
                     EAKVSKCMTRQVRMVGAQTPISTVQENLRQSNAVVVVDDSKRPISIMTKIDLLDYLTT
                     K"
     gene            4320..>5428
                     /locus_tag="PK937_14950"
     CDS             4320..>5428
                     /locus_tag="PK937_14950"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNH33922.1"
                     /translation="MGFSTDAIHAGQIPDPTTGAVITPIYQTSTYAQEELGVSKGFDY
                     GRTINPTRVALEANIAALEKGKYGFAFGSGMAAITNIAALLNGGDHVIVSNNVYGGTF
                     RFFDKIMKRFGLEFSWVDTSDEKNIRAAIKPNTKMLYVETPTNPMLTITDLQVVAALA
                     KEHKLISVVDNTFMSPYFQTPLEFGVDIVLHSTTKYINGHSDVIGGVVVTSSEELKEK
                     LKFLQNSIGAVPSPFDCWLVLRSTKTLALRMRQHNENAIAMAEFLAKHPKVKKIYYPG
                     LASHPQHALAKKQMRGFGGMISIELGSMDAGKNMLRNVKIFALAESLGGVESLICHPA
                     SMTHGAIPLEDRLKLGLTDGLVRLSVGCEDKEDLIA"
BASE COUNT         1421 a         1277 c         1359 g         1371 t
ORIGIN      
        1 cgtattggtg cgcgttaccg tcaaaggaat aaaaggcgga aatcccaaag aaatccaata
       61 ccaaatcgtg gactatggcg atgcgtccaa taatatcacc gccatgatgc gtctgacgtc
      121 ctatccgatt tctgtgatcg cacaaatgat gaccgaaggc aaaatcacgg aaaaaggcgc
      181 cgttccgcag gaattagctg ttccaacacg gtcttttatc gaagcgctca gagcgcgaaa
      241 tattgacatc cagattacag aatcttaagc catcttaatt tgatggttat agcttttgta
      301 gtgaaataaa aaaatattcc agataagtag ccgttatatt tttttttgct taagggatgc
      361 agtgcgccca atctgcgcat gatcgctgtc gtattcttgt tttataccca ccgaatcggc
      421 cctgtttcaa actgatgcag gtagcctcat gaagaaacat tcctaactat cgaatttatt
      481 tgaacttgtg acctgcatca catcgttttg aagcgatgct ttgattttat tgggtttaat
      541 tatccagtca tattttggca aggtatttgt cttatataaa aacaattaac gcgatgataa
      601 cctttcattc actaaaatga ggtaaatcgg atatgagacg caccacttca gcagcacgca
      661 aaacaaagca gaatcccgtg ttgatggatc tggagaatta tattttgaaa ttgggtttga
      721 attccgaacc cacctcggcc aatgtcgttg ccaagcggct tgaactcaaa gagagcccca
      781 aaaatattca gatgatcaag caggctttgg aaaaaatggt gcgtgaaaaa aaggtgtacc
      841 gctgcggaag ccgctatgcg attgattatc agggcggatt caatcttatg aaaacatttt
      901 gcatcaaagt cgcagaagag aattataaaa taaacaattt tgtatttgag ggtgttgctt
      961 acgcccgcgg ggcataattt ttccgattcc tatcggttac cactagggag cggcgcatga
     1021 cttcggttag gcgccgtttc tttttttaag gtagcatgtt tttatttccg gccttatctt
     1081 ttcaaaaaaa ccacggaaga agacgcattc atgcaagagc ttatccgcgt cgaatccctc
     1141 tgtaagtcat acccggtagg gagcacctta tgggggcgtg cgacctggtc gcaagtcgtc
     1201 gaagatgttt cttttatcgt ccgaaccgga gaaacccttg gtgtggtcgg cgaatcgggc
     1261 tccggaaaaa gtacgatcgg gcgatgcctt ctcaaactga ccgatccgac gtcgggacgt
     1321 attttttttg acggcgaaga aattaccgca ttggataata aagcgatgcg tccttttcgc
     1381 cgccgcatgc aaatcgtatt tcaggatccg tttagttcac tcaatccccg ccaaacggtg
     1441 gatgccatgt ttcgtgagat attcaccgtg cacgcgacag cttccggcac ggaggcgaca
     1501 gataaaatcg aagaattgct taaaaccgtc ggtcttgatc cctcacagcg gttttgtttt
     1561 ccgcacgaat tttcaggcgg acagcgccag cgtatcggta tagcacgggc cttgtcggtc
     1621 cggccgcaat tcatcgtttg cgatgaaccc gtttcggctc tagatgtgtc ggtacaggcg
     1681 cagatcatcg aacttttaat gacgcttcag gaaacaagaa atctttctta tctgtttatc
     1741 ggccatgatc tggctgtggt gcgtcatatc gcggatcgga tagccgtctt atatcgcgga
     1801 caaatgatgg aaatgggacc gtcggatgtt ctttttgatc aaccgctgca tccgtacacg
     1861 caggctttga ttgcgtcggt gcccgaaccc gatccgtcca agccggtaaa acccgtcgca
     1921 gtaaaaggtg atatcgcgga ttcgtcgcgc ccttccaagg gttgcctttt tcaggcgcgt
     1981 tgtccgtggg tcgaggccgt ttgccgtgat gaaacgccgc gctggcgttc ttgcgggcag
     2041 gagcgttttg tacgctgcca taaagttaca ggggacaatc cgcattgact ttggcctaac
     2101 tatttatgat atttgcggcg aataatttct cactttttcc aacgattttt aggtgcatca
     2161 tggaggatat tctcaaacgc ctgcgcaatg atacgccggg tgtaagcgcg gttgcgctcg
     2221 tcagtagcga cgggctcgtc atctcgtcca tgctcccgga caccatcgaa gccgaacgcg
     2281 tggccgcgat ttcggccgcg ttgctgaatc agggcgaaag cagttccgaa gacgcgatga
     2341 tcggtgaaat ggcgcaagtc acgatacgcg ccaaagaagg atttatcatc atcacgcggg
     2401 cagccaaaga aacgctgctg accgtcttta ccacaaccca agccaaactc gggcttgtct
     2461 tgtttgacat cagcgtcgcc atcaaagaac ttcaacccca cattcactga ggcatcatga
     2521 tagcatcgca cgatcagtat tttggggaaa tcattcgtac cgttgaggcg attgccccgc
     2581 aaaccttggt ggccgtgatt tcctgggaag gattagtttt gcggtccaat gtatccgatg
     2641 aacacgtcga aggccagttt ggcgctttag cctccgtttt tttagatcag ggcaaacgta
     2701 tgttcactat tccttttgac ggaaaaaaac cgcagccgga caccgtgcag acgatggtca
     2761 cggtcggtac gaaacacgtc atcattatga cccaactttt accggggatt ctattggctg
     2821 tttcctccgg tgataaatcc aaaatcaata cgttccttac ctgttcgctc gaagaagtgg
     2881 acaaactgca cgcgatgatc gcaaaaaaag aaatcatttt ttaatatcta caggaattat
     2941 gaaacggata tacaacaaca ttctcgaagt catcggtcaa acaccggtcg tcaaactcaa
     3001 tcgaatcgga gcacagcttc aatgtaatct gtacgccaaa tgtgaattta tgaatcccgg
     3061 cggttccatc aaagaccgta tcggccgcgc aatggtcgaa gcggcggaac gcgaaggtcg
     3121 tatcaagccg ggagatacgt tgatcgaacc gacaagcggt aataccggta tcggtattgc
     3181 attggccggc gcgatcaaag ggtatcgtgt gatcatcacg atgcccgaaa aaatgagccg
     3241 cgaaaaacaa gtaatactcg aagcgctggg cgccgaaatt attcgcacac cgaccgaagc
     3301 ggcctccgaa gatccggaaa gtcatatcag tgtcgcgcaa cgcctgcaac gcgagttacc
     3361 gaatgcacat attttagatc aatactccaa ccccaataat cccaaaattc actacgatac
     3421 gaccggtcag gaaatcctcg acgcactgga tggcaaagta gatatggtcg tcgttgccgc
     3481 cggtacgggc ggctccatca cgggcatagc caaacgtatg aaagatgcca atccgcagtg
     3541 tattgtcgtc ggcgccgatc cggtaggttc gatcctcggc ggcggtacgc acgtcggtac
     3601 atacaaggtc gaaggtatcg gctatgattt tattcccaaa gtactcgatc gggattgcgt
     3661 ggatgaatgg atcaaaacgg aagatcagtc ctcattttta ttggcgcgcc gcctgattca
     3721 cgaagaaggt ttgctcgtcg gcggttcgtc cggttcatca ctttttgccg cactgcaggc
     3781 cgcacaacgg ttaaagcccg gacaaaattg cgtggtcgtt ttatcggacg gcgtacgtaa
     3841 ctatatgacc aaatttgtgg acgacaagtg gatgcgcgat aacggatttt ttgaaaacaa
     3901 atccattcag ggtacggtca aagatattct agctaaaaat ccggcacgcc ccgtgctgat
     3961 ctgtgccgat gatatggaga gcgtgcgtca gacgatttcc aagatgcgcg aaaaaggtgt
     4021 ttcgcaattg ccggttactt ccggcggcgc attggtcggg atcgtatcgg aaagcgatat
     4081 catggattat ttggccagcg gtgccggtac ccaagaagcc aaagtttcca aatgcatgac
     4141 gcgtcaggtg cgtatggtcg gtgcgcaaac gccgatatct acagtgcagg aaaatctgcg
     4201 ccaatccaac gccgtggtgg tcgtggatga tagcaaacgc ccgatcagca ttatgacaaa
     4261 aattgatttg ctggattatc tgacaaccaa ataataacgc atagtaggaa agaataaata
     4321 tgggtttttc aacggatgcc attcacgccg gccagatccc ggatccgacg accggtgcgg
     4381 tgatcacgcc gatttatcaa acttcgacgt acgctcagga agagcttggc gtcagtaaag
     4441 gttttgatta cgggcgcacg atcaatccca cccgcgtcgc gttggaagcg aatatcgcgg
     4501 cgcttgaaaa aggcaaatac ggtttcgctt ttggcagcgg tatggccgct attaccaata
     4561 tcgccgcact gctcaacggc ggtgatcatg tcatcgtttc caataatgtg tacggcggta
     4621 catttcgttt ttttgacaaa atcatgaagc gttttggtct tgaattttcc tgggtggata
     4681 cgtcggacga aaaaaatatc cgtgcggcta tcaagcctaa caccaaaatg ttgtatgtcg
     4741 agacgccgac caatccgatg ctgacgataa cggatttgca ggtcgttgcg gcactcgcca
     4801 aagaacacaa actgatttcc gtcgtagata atacatttat gagtccgtat ttccagactc
     4861 cgcttgagtt tggtgtggac atcgttttgc acagtacgac caaatatatc aacggccaca
     4921 gcgatgtgat cggcggtgtg gtggtcactt ccagcgaaga gttaaaagaa aaactcaaat
     4981 ttttacaaaa ctctatcggc gccgtaccgt cgccgtttga ttgttggctt gttctgcgtt
     5041 cgacgaaaac tctggcgttg cgtatgcgac agcacaatga aaatgcaata gctatggcgg
     5101 agtttttggc taagcatcct aaagtaaaaa aaatctatta ccccggtttg gcatcgcatc
     5161 cgcagcatgc gctggccaaa aaacagatgc gtggttttgg cgggatgatt tcgatcgaac
     5221 tgggttcgat ggatgccggc aaaaatatgt tgcgtaacgt aaaaatattt gccctcgccg
     5281 aaagcctcgg cggtgtggaa agcctgatat gccatccggc ttcgatgaca catggagcga
     5341 ttccgttgga agaccgtttg aaactcggtt tgaccgacgg tttggtccgt ttgtccgtgg
     5401 gttgcgaaga caaagaagat ttgatcgc
//
LOCUS       DAOQGF010000172         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_7905_length_5322_cov_6.048605, whole genome shotgun sequence.
ACCESSION   DAOQGF010000172 DAOQGF010000000
VERSION     DAOQGF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_5322_cov_6.048605"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PK937_14955"
     CDS             complement(<1..518)
                     /locus_tag="PK937_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNH33923.1"
                     /translation="MSFFPFEFSPEEIRLRDEVRAFTKQYIEPIAKEYDEKSIFPADN
                     VRKMGEHGWMGIPFPKEYGGLGMSTVGYCLAVIELSKVCASHGITLGAHTGIGCGPIW
                     LMGTNEQKLKYLAPLARGEKLGSFGLTEPGAGSDAGGTRTFAENKGDHYVINGSKIFI
                     TSAGYADTFVITA"
     gene            complement(719..1048)
                     /gene="trxA"
                     /locus_tag="PK937_14960"
     CDS             complement(719..1048)
                     /gene="trxA"
                     /locus_tag="PK937_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNH33924.1"
                     /translation="MSHPVVLSDASFENEVIKSDKPVLVDFWATWCGPCRMIAPMVEE
                     LAKEYDGKAKICKIDVDANPAVASKFGIMSIPTLLFFKNGQLADKVVGAVPKAKLVEK
                     LNGVMAK"
     gene            complement(1109..1645)
                     /gene="lspA"
                     /locus_tag="PK937_14965"
     CDS             complement(1109..1645)
                     /gene="lspA"
                     /locus_tag="PK937_14965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNH33925.1"
                     /translation="MKNYWVAIIIVILDQITKYIVKETMILHDSIPLWDTTIQLTYLE
                     NPGMAFGIRFFEDHPFWGRWFFSIVSIVASGALIAYIHSMRHEKWPYRFSLSLILGGA
                     VGNLIDRVMFGRVVDFMDVDIPDMLGMQRWPVFNVADSAVVLGMIIMTIFVLTHKPAS
                     NTVNETSGTDTPATGGIN"
     gene            complement(1660..2058)
                     /locus_tag="PK937_14970"
     CDS             complement(1660..2058)
                     /locus_tag="PK937_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNH33926.1"
                     /translation="MKKKDLEYFKKLLQEEINKVLKDMGYIKETLLEETSREAAGDHS
                     GYSFHMADQGSDTHDREKQFMLAARENKYLSDLADAMERVKAGTYGVCMICNKDIEKE
                     RLEMVLVAKYHVACKKKLEEVKQKEHQTES"
     gene            complement(2099..5239)
                     /gene="ileS"
                     /locus_tag="PK937_14975"
     CDS             complement(2099..5239)
                     /gene="ileS"
                     /locus_tag="PK937_14975"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNH33927.1"
                     /translation="MFKEIESQDELAKLSAEVLDFWKKNRIFERSVEERDPKKPFVFF
                     EGPPTANGRPGIHHVLARSMKDFVCRYKTMQGYRVERKGGWDTHGLPVEIEVEKSLKL
                     SGKKDIEAFGMEAFNKKCFESVWTYKELWDQLTDRMGYWVDLKDPYITCDNNYVESVW
                     WILKQYFEKGLVYKGFKVVPYCPKCGTPLSSHEVAQGYQDVSDPSVYVKMKVHGHENT
                     YFLVWTTTPWTLISNVALAVGKDIDYVKAKYKDQFLILAEARVKDVLKEDYEIVERFK
                     GAHILGWEYERLFDDIPVDKKAFFVIPGDFVTTDDGSGIVHTAPAFGEDDYQVCKANG
                     LPFLRPVDAAGKFEETVKEFKGMFVKDADPEIITNLKRRGMLFRSEKFKHSYPHCWRH
                     RTPLIYYARDAWYIRTTTYKDNMVANNETVQWFPPEIKEGRFGNWLRNNIDWSLSRDR
                     YWGTPLPIWVHEESGEMICVGSREELQKGRMKDGSPVPADLDLHKPYVDHVVIEKDGK
                     TFTRTPEVIDVWFDSGAMPYAQWHYPFENKEIFERQYPADFICEGVDQTRGWFYSLMS
                     ISTFLFGKAPYKNVVVNELVLDGQGRKMSKSLGNVVNPFEMLDQYGADILRWYLLVNT
                     PIWLPKRFDLEGLTEMKKKFFRPLLETYKFFTLYANIDGFDPKAASIALSDRTELDRW
                     ILSRLHSTVREVTEHLNQFDTCKAGKLIQEFVDQDLSNWYIRLSRRRFWKGEKGSDKT
                     AAYQTLFEILETLSRLIAPFCPFLAEKMYRHLMQNEDDKTSVHLAYFPEAEKAKMDLA
                     LETKMSSPRRVVQLGRALRELKELKVRQPLAKIIVVTEKESVRGHINDFSDIIKNELN
                     VKAIEFVTDTGSLVNRKAKPNFKALGPKFGKNVNMAAQAIKDFSEKEISLLIEKDHYD
                     LNVAGHEVAITKADVEILHEEKEGLAVQSDADLTVALDTHLTAELVDEGIAREFINRV
                     QNLRKDADFEVTDRIIITLQAEPTILKAIQNQKNYIMNETLATEISVQSPEGEIVRGA
                     ELHDTTLEIGLRRVRG"
BASE COUNT         1328 a         1278 c         1210 g         1506 t
ORIGIN      
        1 gcggtgataa caaaagtgtc tgcgtaaccc gccgaagtga taaaaatttt agaaccgttg
       61 atcacataat gatcgccttt attttcggca aacgtgcgtg taccgcccgc atcgctgccc
      121 gcgccgggtt ccgttaatcc gaaagaaccg agcttttcac cgcgtgccag cggcgcaaga
      181 tatttcagct tttgttcgtt cgtacccata agccagatcg ggccgcaacc gatgccggtg
      241 tgcgcgccga gcgtgatacc gtgcgaagcg catacttttg aaagttcgat cacagccagg
      301 cagtagccga ccgtggacat gcccagtccg ccgtattctt taggaaatgg aatgcccatc
      361 catccgtgtt cgcccatttt gcgaacattg tcggcgggaa agatggattt ttcatcatat
      421 tctttggcta tcggttcgat atattgtttg gtaaacgcac gcacttcatc gcgaagacgg
      481 atttcttccg gcgaaaattc aaacgggaaa aacgacatat tccgaagatc ctttcagaga
      541 gaaaatttga cgccgtgaat atagatacgg tggtcggtat aatcaagaag ttagcgggtt
      601 agtgttcatc taaaaacaaa agccgttcca tgcgttgaac ggcttttgta gtttatgaat
      661 gtttcgttac ttccgcatgc gcggtgtagc ggtatgcccc gtaggggcga aagttttttt
      721 atttggccat tacaccgttg agtttttcaa cgagtttggc tttggggacg gccccgacta
      781 ctttatccgc aagttgacca tttttgaaaa acagcaacgt cggaatgctc atgatgccaa
      841 acttggaagc cacggcggga ttggcatcca cgtcaatttt acagatttta gctttgccgt
      901 cgtattcttt ggccaattct tcaaccatag gcgcgatcat acggcacggg ccgcaccacg
      961 ttgcccaaaa atctaccaat acaggtttat cggatttgat aacttcgttt tcaaagctcg
     1021 catcgctcaa tactacagga tgactcataa tttaactcct catttcgcta tattttcagt
     1081 tcgtgcaaat cgtgtaacgt agtaagtttc agtttattcc acccgtagcg ggtgtatcgg
     1141 ttccgcttgt ttcattaacc gtgttcgacg ccggcttatg cgtaagcaca aaaatcgtca
     1201 taatgatcat accgagaacg acggcggaat cggcaacatt gaataccggc cagcgttgca
     1261 tacccagcat gtccggaata tccacatcca tgaagtccac tacgcgtcca aacatcacac
     1321 ggtcaatcag attgcctacc gcaccgccaa gaatcagcga gagggaaaag cggtaaggcc
     1381 atttttcgtg acgcatagaa tggatgtatg cgatcagcgc gccggatgcg acgatcgaaa
     1441 cgatcgaaaa aaaccaacgg ccccaaaacg gatgatcttc aaaaaaacga ataccgaaag
     1501 ccataccggg attttcaaga tacgtcagtt gtatcgtcgt atcccagaga ggaatactgt
     1561 cgtgcagtat catcgtttct ttcacgatat acttggtgat ctgatccagt atgacgatga
     1621 tgatcgctac ccaatagttt ttcaaaatcg ttatctttct taggattcgg tctgatgttc
     1681 tttttgtttt acttcttcca atttcttctt gcacgcgacg tgatacttgg cgacaagtac
     1741 catttcgagg cgctcctttt cgatatcttt attgcatatc atacatacgc cgtacgtgcc
     1801 ggctttgacg cgctccatcg catcggctaa gtcggacaaa tatttatttt cacgtgccgc
     1861 aagcatgaat tgtttttcgc gatcatgcgt atcgctgcct tgatcggcca tgtggaacga
     1921 ataacccgag tgatcgccgg ccgcttcgcg cgacgtttct tcgagcaatg tctccttgat
     1981 ataacccata tccttcagga ctttattgat ctcttcctga agcagctttt tgaagtattc
     2041 aaggtccttc ttcttcatga taacccctct cgtttatgtg aactatcttt ttttcaattt
     2101 atccgcgtac acgacgcaag ccgatctcaa gcgtcgtatc atgaagctcc gcaccccgca
     2161 cgatttcacc ctccggtgat tgaacggaaa tttcggtagc caatgtttca ttcataatat
     2221 aatttttttg attctgaatt gctttcaaaa tcgtcggttc agcctgtagc gtgatgataa
     2281 tgcggtcggt tacttcaaaa tccgcgtctt tgcgcaggtt ttgtacgcga ttgataaatt
     2341 cacgcgcaat gccttcatcc acgagttccg ccgttagatg cgtatccagg gctaccgtca
     2401 gatcggcatc cgattggacg gccaaacctt ctttttcttc atgcaggatt tcgacatcgg
     2461 ctttggtgat cgccacttca tgaccggcaa cattcagatc ataatgatct ttttcgatca
     2521 ggagtgaaat ttctttttcc gaaaaatctt tgatcgcttg tgccgccata ttgacattct
     2581 taccgaattt cgggccaagt gctttaaaat tgggcttagc tttacgattg accaaactgc
     2641 cggtatcggt tacaaattct atagccttga cgttgagttc atttttgatg atgtcactga
     2701 aatcattgat atggccgcgc accgattctt tttcagtgac cacgatgatc ttggccaaag
     2761 gctgacgcac tttcagttct ttgagttcac gcaaggcgcg tccgagttga acgacgcgac
     2821 gcggagaact catcttggtt tctaaagcca aatccatttt ggccttttcc gcttcgggaa
     2881 aataggccaa atgaacgctg gttttgtcat cttcgttttg catcaggtgg cgatacatct
     2941 tttctgcaag gaaaggacaa aagggtgcga taaggcggct caaggtttca agtatttcaa
     3001 aaagagtctg gtatgccgcc gttttatcgc tgcctttttc gcctttccag aagcggcggc
     3061 gcgacagacg gatgtaccaa ttggataaat cctgatcaac aaattcctga atcaatttac
     3121 cggctttgca ggtgtcaaat tgattcaaat gttccgtcac ttcgcggaca gtgctgtgca
     3181 ggcgcgataa aatccaacga tcaagttccg tacgatcgct caacgcgatg gatgcggctt
     3241 tgggatcgaa gccgtcaata ttggcataga gcgtgaaaaa tttgtacgtc tcaagcagcg
     3301 gacggaaaaa tttctttttc atttccgtca agccttcgag gtcaaatcgt ttggggagcc
     3361 agatcggcgt attgaccaaa agataccagc gtaaaatatc ggcgccgtac tgatccagca
     3421 tttcaaaagg attgacgaca ttgccgagcg acttactcat tttgcgccct tggccgtcga
     3481 gcacgagttc gttgactacg acatttttat acggcgcttt gccgaataaa aacgtagaaa
     3541 tggacatcag cgaataaaac caaccacggg tctgatccac gccttcgcag ataaaatcgg
     3601 cgggatattg gcgttcaaaa atttctttat tctcaaacgg ataatgccac tgtgcatagg
     3661 gcattgcacc cgagtcaaac catacgtcaa tgacttcggg ggtgcgggta aatgttttcc
     3721 cgtctttctc gatcacgacg tgatctacat aaggcttatg caaatcaaga tcggccggta
     3781 cgggcgaacc gtctttcatt cggccttttt gcagttcttc tcgactgccg acgcaaatca
     3841 tttcgccgga ctcttcgtgt acccatatcg gaagcggggt tccccaataa cgatcgcggg
     3901 ataacgacca atcaatatta ttgcgtaacc aattgccgaa gcggccttcc ttgatttccg
     3961 ggggaaacca ctgcaccgtt tcgttattgg ccaccatgtt gtctttatag gtcgtcgtgc
     4021 ggatatacca cgcatcgcgg gcgtagtaga tcaacggtgt acgatgacgc cagcagtgcg
     4081 gatagctgtg tttgaatttt tcagagcgga aaagcatgcc ccggcgtttg agattagtga
     4141 tgatttccgg gtcggcatct ttgacaaaca tgcctttgaa ttctttgacg gtttcttcga
     4201 attttcctgc ggcatccacc ggacgcagaa aaggcaatcc gttggcttta catacttggt
     4261 aatcgtcttc gccgaaagcc ggcgccgtat gcacaatgcc ggaaccgtca tcggtcgtaa
     4321 caaaatcgcc agggatcaca aaaaacgctt ttttgtcaac cgggatatca tcaaatagac
     4381 gttcgtattc ccagccaaga atgtgtgcgc ctttgaaacg ttcgacaatt tcataatctt
     4441 cttttagtac atctttgacg cgggcttcgg cgagaatgag aaactgatct ttatatttgg
     4501 ctttgacgta atcaatatct ttgccgacgg cgagcgctac attggaaata agcgtccatg
     4561 gcgtcgtagt ccacaccagg aaataggtgt tttcatggcc gtgcactttc atttttacat
     4621 agacggaggg atcgcttaca tcctgataac cctgggccac ttcgtgggac gaaagcggtg
     4681 taccgcattt ggggcaatag ggaacaactt tgaagccttt gtacactaaa cctttttcaa
     4741 aatactgctt gagaatccac cacacggatt cgacgtaatt attatcacag gtgatatacg
     4801 gatctttgag atccacccaa tagcccatgc ggtcggtcag ttggtcccac aattccttat
     4861 acgtccagac gctttcaaaa cattttttat tgaaggcctc catgccgaac gcttcgatat
     4921 cttttttgcc ggataatttg agcgattttt caacttcgat ttcgacggga agtccgtgcg
     4981 tatcccagcc gcctttgcgc tcgacgcgat aaccttgcat cgtcttgtag cggcaaacaa
     5041 aatctttcat tgaacgtgcc agtacgtgat gaatgccggg gcggccattg gccgtcggag
     5101 gtccttcgaa aaacacaaat ggttttttcg gatcacgttc ttcgacactg cgctcaaaaa
     5161 tgcggttctt cttccagaag tccaacactt cggcggataa tttggctaat tcgtcttgcg
     5221 attcgatttc tttgaacata ctgagtaccg tatttgctcg agaagcgtaa gttgttgtca
     5281 attttttaaa taaattgagg ggcatatata taaaaagaag gg
//
LOCUS       DAOQGF010000173         5218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_8170_length_5218_cov_6.903738, whole genome shotgun sequence.
ACCESSION   DAOQGF010000173 DAOQGF010000000
VERSION     DAOQGF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8170_length_5218_cov_6.903738"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PK937_14980"
     CDS             complement(<1..105)
                     /locus_tag="PK937_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNH33928.1"
                     /translation="MCSLTNRIAVVTGSSRGIGKAIAVEFLKHGASVVI"
     gene            complement(142..1080)
                     /gene="fabD"
                     /locus_tag="PK937_14985"
     CDS             complement(142..1080)
                     /gene="fabD"
                     /locus_tag="PK937_14985"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNH33929.1"
                     /translation="MSKIAFVFPGQGSQYVGMGKDLYDLFPTVKAMYETANRIIGPDF
                     SKICFEGPEDALKQTRITQPAIFVHSVALLEVLREKFGTDKVTFHMTAGHSLGEYSAL
                     VAAGVLTFEDALAVVKVRAEAMQRAGEINKGTMAAVIGMEPEPLVSICKTASSEAGVV
                     QCANFNSPGQIVISGSVAGVQKAMEMAKAQGVRMVKELVVSGAFHSPLMEPARAEVQN
                     KLKTITLSDARLPVYANVSAEPTTDAQRVQSLLVEQITGAVRWQESVTRMIADGATKF
                     FEIGPGNVLQGLIKRIDKSVSCECIGKADELAKFVI"
     gene            complement(1153..2157)
                     /locus_tag="PK937_14990"
     CDS             complement(1153..2157)
                     /locus_tag="PK937_14990"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNH33930.1"
                     /translation="MALGNKYRAKISGVAHWVPEQILSNKELEKMVDTTDEWITTRTG
                     IKERHILEKGKATSDLAYEACKRLLEQTNTDPAEIDLIIVGTVTPDMFFPATACLLQA
                     RLGASKAWGYDVSAACCGFAYSLVSGTQFIESGTAKKVIVVGADKMSSIIDYTDRNTC
                     ILFGDGAGAVLLERSEEDTIGILDYYNTVDGNGAQYLNMPGGGSLNPPSHETVDKKMH
                     YVFQDGKNVYQYAVKGMSEASALIVERNGYTGKDVALFVPHQANKRIIDSAANRMGLD
                     ESKVVINIDRFGNTTAGTIPIALSESVSNGRLKKNDLVVIAAFGGGFTCSSILLRWAY
                     "
     gene            complement(2203..3219)
                     /gene="plsX"
                     /locus_tag="PK937_14995"
     CDS             complement(2203..3219)
                     /gene="plsX"
                     /locus_tag="PK937_14995"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNH33931.1"
                     /translation="MKIVVDAMGGDEAPKHIIDGVILAARAWVSTGVNHEIILTGDKD
                     VVMKEIERCGGRDLCGNILRLEHTACVIDMHESLADAWRNKSGSSIHRGAELVKSGQA
                     DAFIGMGNTGAMMTAGLLGVGRIEGVQRPTIGAFFPTARGRSLVLDVGAVTDCKPQHL
                     LQFGLMGSIYMEAMWGVKNPTVGLLSIGEEDSKGNEVTKETNALFREKKLFNFAGNVE
                     GRDILKGTTDVIVCDGFVGNIVLKFGESVPGFLKARFKQAAESSFMTKLQLLLAKTGI
                     KGVFKDMNYEEYGGVPLLGVNGVVIIGHGSSSPKAMYNAIMVAQKMVDKKINEVIKER
                     IRLS"
     gene            complement(3379..3561)
                     /gene="rpmF"
                     /locus_tag="PK937_15000"
     CDS             complement(3379..3561)
                     /gene="rpmF"
                     /locus_tag="PK937_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNH33932.1"
                     /translation="MPNPKRRHSKQRRDKRRTHWKLDNAAVGKCANCGQPKMHHRVCP
                     NCGHYDGRQIVIAREA"
     gene            complement(3590..4087)
                     /locus_tag="PK937_15005"
     CDS             complement(3590..4087)
                     /locus_tag="PK937_15005"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNH33933.1"
                     /translation="MVAKIDKILSLGDGEHRFHFDVKAEDIGLNSIDFEGRNHFDKPI
                     SADAVLTKTNHVYYIKMKVVGEARLVCDRCLEDVSVDVADDFQVIYTDHRSERYAQSG
                     ETEIRLLDVRKENQIVLDKDIVDTIELAIPHKVLCDEDCKGLCVQCGANLNERACEHA
                     QTVNE"
     gene            4534..>5218
                     /locus_tag="PK937_15010"
     CDS             4534..>5218
                     /locus_tag="PK937_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33934.1"
                     /translation="MHTFIQKFVWLPHDPGLPPLEFWRKRITIAVVAANCIMGTAALI
                     PTIPFFMQSETPWIGLIFVVMMIGMYFAWFSQKPSQLKKSILVTVSFLIAGTLCFKPF
                     GAAGAICTLFVAHILSSLLFRPRFFLISLFYILIVFIAAGYYMEAGNVIGEDRFESRH
                     TWNTWFIVIANVTAAFLTTGWAIHVVINSLEHSLIMENTSRIALQEEINQHNRTEFER
                     KKLEEEVFHM"
BASE COUNT         1393 a         1348 c         1016 g         1461 t
ORIGIN      
        1 gatcacaacc gatgcgccgt gttttaaaaa ttctacagca atggccttgc cgataccacg
       61 ggaggagccg gtcactacgg caattcgatt cgtcaaagaa cacatggcgt tcgcctttgc
      121 ttatgcacaa taaaggcaac gttatattac aaatttagct aattcatcgg ctttaccgat
      181 gcattcgcag gatacacttt tatcaatgcg cttgatcaat ccctgcaaaa cattaccggg
      241 accgatttca aaaaacttag ttgcgccgtc ggcgatcata cgcgttacgg attcttgcca
      301 gcgcacagca cccgtaattt gttctaccag caaagactgt acacgctgag catccgtcgt
      361 gggttccgcc gatacattgg catacaccgg taaacgcgca tcgctcagtg ttatggtttt
      421 aagtttgttc tgcacttcag cacgcgccgg ttccatcaaa ggtgaatgga aagcgccgct
      481 cacgactagc tcttttacca tacgaacgcc ttgggccttg gccatttcca tagctttctg
      541 cactccggct acggaaccgg aaatcacaat ctgtccgggg ctgttaaaat tggcacactg
      601 cacgacgcct gcttcgctgc tggcggtttt gcaaatagac accagcggtt caggttccat
      661 accgataacg gccgccatcg tgcctttatt gatttcaccc gcacgttgca tcgcttcggc
      721 tcgtactttg acaacggcta aagcgtcttc aaaggttaac acaccggcgg caaccaaagc
      781 ggaatattcg cccaaactgt gccccgccgt catatgaaat gtcactttat ccgttccaaa
      841 cttctcacgc agaacttcca gtaatgccac gctatgcaca aaaatcgccg gttgtgtaat
      901 gcgcgtttgt ttcagcgcat cttccggccc ttcaaaacaa attttcgaaa aatcaggacc
      961 gataatccgg ttggccgttt catacatagc cttgacggtc ggaaacagat cgtacaaatc
     1021 tttacccata ccgacatact gtgaaccctg cccgggaaat acaaaagcga ttttactcat
     1081 acgattttta tttatttatt atttttatcc atgacagtac aagccaacgg attcgtcaca
     1141 ttccgctaat tattaatacg cccagcgcaa caaaatactg ctgcatgtaa atccgccgcc
     1201 aaatgcagcg atcacgacca agtcattttt cttaaggcgc ccattgctga ccgactccga
     1261 taaggcaatc gggatggtgc cggccgtggt attaccgaaa cgatcaatat taatcacaac
     1321 tttactctca tccaatccca tacgattggc cgcactatct ataatacgct tattggcttg
     1381 atggggcaca aaaagcgcta cgtctttacc ggtataaccg tttcgttcga cgatcaacgc
     1441 agacgcttcg ctcatacctt tgacggcata ttgatataca tttttcccgt cttgaaaaac
     1501 ataatgcatc tttttgtcaa ccgtttcatg cgaaggcgga ttaaggctgc cgccgccggg
     1561 catattgagg tactgcgccc cgttaccatc tacggtgtta taataatcta aaataccgat
     1621 cgtatcttct tcagaacgct caagtaaaac ggcgccggcc ccgtcgccga acaatataca
     1681 ggtattgcga tcggtataat caatgatcga actcatttta tcggcgccca cgacgattac
     1741 ctttttggcc gtaccggatt cgataaattg agtgcccgat accaatgagt aagcaaagcc
     1801 gcagcatgcc gcggatacat catagcccca ggccttggaa gcaccaagtc gtgcctgtaa
     1861 aagacatgcg gtcgccggaa aaaacatatc cggcgtaacg gtccccacaa tgatcaaatc
     1921 aatttcggca ggatctgtat tcgtttgttc caaaaggcgt ttacaagctt cataggcaag
     1981 atcggatgtg gctttacctt tttctaaaat atgtcgttct ttgataccgg tccgcgtggt
     2041 tatccattca tcggtggtat ctaccatttt ctcaagctct ttattgctga gaatttgctc
     2101 gggcacccag tgggcaacac ccgagatttt tgcacgatac ttattgccta acgccataat
     2161 tcttttctac tgcggcatgc agcacagcgc gcagtgtgtt agttatgata atcgaatacg
     2221 ttctttaata acttcgttta tttttttatc caccattttt tgcgcaacca tgatcgcgtt
     2281 gtacatggct ttgggcgaag aactgccgtg accgatgatc accacgccgt ttacacccaa
     2341 caaaggaacg ccgccatatt cttcataatt catatctttg aaaacaccct tgatgccggt
     2401 ctttgccaac agcaattgga gtttcgtcat aaaactcgac tcagcggcct gtttgaaacg
     2461 ggctttgaga aaaccgggaa cgctttctcc aaattttaat acgatatttc ctacaaaacc
     2521 gtcgcacacg atgacatccg tcgttccctt gagaatgtcc cgaccttcca cattacccgc
     2581 aaaattgaat aatttttttt cacgaaacaa agcattggtt tctttagtta cttcatttcc
     2641 tttggagtct tcttcaccga tactcaaaag ccctaccgtg ggatttttta caccccacat
     2701 cgcctccata taaatactac ccatgaggcc aaactgcaaa agatgttgcg gtttacaatc
     2761 cgtgacggcg cccacatcga gcaccagaga gcgcccgcgg gctgtcggga aaaaggcacc
     2821 tattgtagga cgttgaacac cttcgatccg tccaacgccg agtaatccgg ccgtcatcat
     2881 tgccccggta ttgcccatcc cgataaacgc atccgcttgg ccggatttga ctaattccgc
     2941 accgcgatga atcgaggagc cggacttatt tcgccaagcg tctgccagtg actcatgcat
     3001 atcgatgaca catgccgtat gttctaagcg taaaatattg ccgcataaat cacgacctcc
     3061 gcagcgctcg atctctttca ttactacatc tttatcgccg gtcagaatga tttcgtgatt
     3121 tacgccggta gatacccatg cccgagccgc caaaatgacg ccgtcaatta tatgtttggg
     3181 cgcttcatca ccgcccatcg catccacaac gatcttcatt accttctccg acttatatgc
     3241 ctgttcgttt cctattacga aaagaccgcg ccaatatacc gttatcaacc cacattatca
     3301 aaccgcaatt aaaaaaaaac ctgtcgttgc cgtgggaaca aggacaggtt tacttagacg
     3361 atttgagcgc actccaaatc aggcttcgcg cgcgatcacg atctgacggc catcgtagtg
     3421 tccgcagttg gggcacacac ggtgatgcat tttgggttga ccacagttgg cacatttgcc
     3481 gacggcggca ttatccagtt tccagtgcgt acgacgttta tcacggcgct gctttgaatg
     3541 tctgcgtttg ggattaggca tgatattctc ctaaaataat gtaaaatcgt tattcgttca
     3601 cggtctgcgc atgctcacat gcacgttcat tgagattcgc tccgcactgt acacacaaac
     3661 ctttacaatc ttcatcacaa agaactttat gcggtatcgc aagctcaatc gtatctacaa
     3721 tatctttatc tagaacgatt tggttttcct tgcggacatc caataaccgt atttccgttt
     3781 caccgctctg cgcgtaccgc tcgctccgat gatcggtgta gatgacctga aaatcatcgg
     3841 ccacatctac acttacatcc tccaaacaac ggtcgcatac taatctcgcc tcgccaacca
     3901 ctttcatctt tatataatag acgtgattgg ttttggtcaa caccgcgtcc gccgaaatcg
     3961 gtttatcgaa gtgattccgg ccttcaaaat caatactgtt caagccgata tcttcggcct
     4021 taacatcaaa atggaaacgg tgttcaccgt ctcctaagct taaaattttg tcaattttcg
     4081 caaccaagcg aacacctaac ttcaatttga taaaacactt atcaaatttt ggggcacgaa
     4141 gatagttttt tttgtttgga ttgtcaaggg aaaagaggaa ctttcaagct ctgtatatat
     4201 agctgcccca ttaacgtcta tatcgcatcc tacagcaaat caatttattc agccaacttc
     4261 aatatttggt ctctcatcgg ctgaaaaaaa atttgttttt ggcgcaaaat aagatgttca
     4321 acctttttaa tttcagacgg ttaattaata tgttcaggca ctcaatcatg cacaaacata
     4381 aaattctaaa acaaatccac aaaacataca ttgatatttt tcacacaaac taacccacgc
     4441 aaaaacagct acatcttgat gtaggttgat gttcttacct gaaaatcgga ttttcattac
     4501 ccaatttaca cctccgcaac atcattaaat tcaatgcaca cattcatcca aaaattcgtt
     4561 tggttacctc acgatcccgg cctccctccg cttgaattct ggagaaaacg catcactatc
     4621 gccgtagtag ctgccaactg cattatggga accgccgcat tgatccctac aattccattt
     4681 ttcatgcaaa gcgaaacgcc atggataggc cttattttcg ttgtgatgat gatcggcatg
     4741 tatttcgcgt ggttttctca aaaaccatca caattaaaaa agtctatttt agttacagtc
     4801 agctttttga ttgccgggac attgtgtttt aaaccctttg gtgccgcggg agcaatatgc
     4861 actctattcg tcgcacatat cttatcgtca ttactttttc gtccgcgctt ttttcttata
     4921 agcctttttt acattcttat cgtctttatt gcggccggtt attacatgga agcaggaaat
     4981 gttatcggtg aagatcggtt tgaatcgcgc catacatgga atacctggtt tatagtgata
     5041 gccaatgtta ctgcagcttt cttgacgacc ggttgggcca ttcacgttgt gatcaatagt
     5101 ttagaacatt ccttgatcat ggaaaataca tcccgtatag cgcttcaaga ggaaatcaat
     5161 cagcacaatc gaacggaatt cgaacgtaag aaattagaag aagaagtgtt ccatatgc
//
LOCUS       DAOQGF010000174         5173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_8297_length_5173_cov_6.563697, whole genome shotgun sequence.
ACCESSION   DAOQGF010000174 DAOQGF010000000
VERSION     DAOQGF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8297_length_5173_cov_6.563697"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK937_15015"
     CDS             <1..200
                     /locus_tag="PK937_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33935.1"
                     /translation="SYPQSGSVSFTIKSNFEGDEIEGDADSLNYYIEAKITFDGDNTA
                     LLEFGGYSFTLNLDSFTITPI"
     gene            331..1551
                     /locus_tag="PK937_15020"
     CDS             331..1551
                     /locus_tag="PK937_15020"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH33936.1"
                     /translation="MRLFLSDWVLPVVSPPIRYGGVVTDQGKIIAVGEASTLQDKFPS
                     IVVERMTNTVIMPGLVNAHTHIELSMMKGAIPDGLPFVEWVYRAITGRFTYTTEAIRG
                     AASLAVQSMIQNGTVAVGDIANNSDVSQPLLENSNMWAVIFHEATGFQSALALEKFNE
                     FSRKAWQPGTERVRHALAPHAPYSVSRALFSEIRAFNRSGRLTTMHLAESHDEVVFIR
                     TGEGPMREMIEKLGRWDDTWEAPKTTPVKYAHALGMLMPGMLAVHVVHADDEDIQLMK
                     QFHISVCSCPRSNVKINVGGTAPIRKYLDAGINVCIGTDSLASNDDLNVWNEMHALQK
                     QHASVKPEEIVRIATFNGAKALSLDNVIGTIETGKADALIAVTSRESVSEPFGFLTGG
                     KSTFDSVRPLFAHS"
     gene            1603..2622
                     /gene="aroF"
                     /locus_tag="PK937_15025"
     CDS             1603..2622
                     /gene="aroF"
                     /locus_tag="PK937_15025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNH33937.1"
                     /translation="MMIVVMHRDASVSDISTVLHIIEESDCTAHVSKAEEKTVVGVVG
                     DDRGIVSEKIQTLQGVEKIFATSKPYKLASREWKKENTLVRVGQVTFGGSDVVLIAGP
                     CGVESEQQILEIAQAVKEAGAHILRGGAFKPRTSPYSFQGLGERGLELLALAREKTGM
                     PIVTEVVTPNQVELVAKYADMLQIGARNSLNYSLLQEVGKTNKPVLLKRGMMSHLEEY
                     MMSAEYILANGNYNVVLCERGIRTFETSTRNTMDIAAIPVLKSWTHLPVIADPSHATG
                     KRSLVQPVAKAAVAAGADGLMIEVHHHPEHALSDGPQAVLPDEYAALVRQLRQISSVL
                     GRSIA"
     gene            2651..3232
                     /locus_tag="PK937_15030"
     CDS             2651..3232
                     /locus_tag="PK937_15030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNH33938.1"
                     /translation="MKVSTVTTRYSYLEESSLSSEKLVDGHLLKAWRDVVQLPDGKTG
                     IREYIRHPGAVIMLPLFENGDTMLIRQFRFPPKKIFWELPAGKLDGPEDPLVAAQREL
                     IEETGWSASHWTRVGSFYPCIGYSDERMILYIAEDLTPAESAHDDDEFIERFRMPRGD
                     ALQKVWSGEIDDMKTIAALLLAEHTLRNNSKTR"
     gene            3236..>3933
                     /locus_tag="PK937_15035"
     CDS             3236..>3933
                     /locus_tag="PK937_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33939.1"
                     /translation="MGTFDRDDKDGWREREGDEEEPWKKAHENWKNDSRLSDDVPATR
                     ETSPFWTEERWEHFIQETDKDGLQVIKYYERYWNHQDRDVMVENAMALHYVREAFKYI
                     HPENHKSFYVHYFLEPECALMKLPVQSSGHFLEAKLRTVSDIQAYRLSRDFARFAYRW
                     LKDLPDELRMEKVSESFTSNILNVCTKLSGGHVMGYQPWTMGGQIVLTKRALRSINRC
                     MEILFTVKDQWALD"
     assembly_gap    3934..4033
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4034..>5173)
                     /locus_tag="PK937_15040"
     CDS             complement(<4034..>5173)
                     /locus_tag="PK937_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH33940.1"
                     /translation="ETMVRAWMQWIFSLCQHFPLLVLYSAVLFILSWKITLLSLVLIP
                     GITLLQHRLQKYTARWALFLHERESEIQSDVWHKLQAFKLIRLFHSAGYEAKRFSSLE
                     SRRSDAATRRDRLQAIVLATVEMAGVSVGVMLLLTIGSWTMEGEFMYGPGGFVLFIAA
                     AFSIIDPARHIARGRMFLSEARTLWDEYKNKPMASSEETSPTKDIASFTNALCLEETC
                     FAHRTTEILNKVNITIRRGECVIITGKTGAGKSTILDLIIGLYAPQRGCITLDGIPVT
                     QISHATRAKLFGLMTQEPLIMHATVRDNVVYDQIHVSDEHVIEALTHVQLDTWIKTLP
                     DGLDTIIGEKGWTMSGGERQRLALARIVLRKPEILLLDEATSALDE"
BASE COUNT         1354 a         1167 c         1258 g         1294 t
ORIGIN      
        1 attcgtatcc gcaaagcggt tcggttagtt ttacgatcaa atcgaatttt gaaggagatg
       61 agattgaagg tgacgccgat tcacttaatt attacattga agctaagatc acttttgatg
      121 gtgataacac ggctttatta gaatttggcg gatacagctt tacgctcaat ttggatagct
      181 ttacgattac accgatctga tttggatttc atattttttc gaataaccgc ttcggttcgc
      241 acagagccgg gcggtttttt attttgtatc aaagaatcat gagatgtagt aaattctaca
      301 ttttgagaga aaaaaggaga tcggcgtttt atgcgtttat ttttgtctga ctgggtatta
      361 cccgtggtgt ctccaccgat tcgctatggc ggcgttgtta cagatcaagg aaaaataatc
      421 gccgtaggtg aagcttctac acttcaagat aaatttccat ccatcgtcgt ggagcggatg
      481 acaaatacgg taatcatgcc gggtttggtc aatgcacaca cccacatcga actgtcaatg
      541 atgaaaggtg ctattcccga tgggctccca tttgtagaat gggtgtatcg tgctatcacg
      601 ggacgtttta cttatacaac tgaagctatt cggggcgcag catcactagc tgtacaaagt
      661 atgatccaaa acggtactgt ggctgtgggc gatattgcca ataattccga tgtatcgcag
      721 cctcttttgg aaaattccaa tatgtgggct gttatctttc acgaagcaac cggatttcaa
      781 tcagccttgg cgcttgaaaa atttaacgaa ttttcccgta aagcatggca gcccggcacc
      841 gaacgcgtgc gtcatgcgtt ggccccgcat gcgccatact cagtatcacg cgcactattt
      901 tccgaaattc gcgcattcaa tcgtagtggc cgtctgacaa cgatgcattt ggcagaatcg
      961 cacgatgagg tggtctttat acgaaccggc gagggtccga tgcgggaaat gatcgaaaaa
     1021 ctcgggcggt gggatgatac gtgggaagcg cccaaaacca cacccgtaaa atacgcccac
     1081 gctttgggta tgctgatgcc gggtatgctg gccgtgcatg tagtgcacgc ggatgatgaa
     1141 gatattcaac tgatgaaaca gtttcacatc tcggtatgct catgcccgcg aagcaatgta
     1201 aaaatcaatg tcggcggaac ggcaccgatc agaaaatatc tcgatgcggg tatcaatgta
     1261 tgcatcggca cggacagttt ggcaagtaat gatgatctga atgtgtggaa cgaaatgcat
     1321 gctttgcaaa aacagcatgc ctctgtgaaa ccagaagaaa tagttcgtat cgctacgttc
     1381 aacggcgcca aagccctaag tcttgataat gtgatcggca cgatcgaaac cgggaaagcc
     1441 gacgccctca ttgcggtgac ttcacgcgaa tccgtgtcgg aaccgttcgg tttcctgacc
     1501 ggcggaaaat cgacgtttga ttcagtacgg ccgttgttcg cccattcttg atcggtgtat
     1561 tttttttgcg tagattgtga caatcgaaag gtgattttat ttatgatgat tgtagtgatg
     1621 catcgcgatg catcggtttc ggatatttcg accgtccttc atattatcga agagagcgat
     1681 tgcacggcgc atgtcagcaa agcggaagaa aaaaccgtcg tcggtgtcgt tggcgatgat
     1741 cgcggtatcg tgagtgaaaa aattcagacc ttacaaggtg ttgaaaaaat atttgcgaca
     1801 tctaaaccgt acaaattggc cagccgtgag tggaaaaaag aaaatacact ggtacgtgta
     1861 ggacaggtta cgttcggcgg ttcggacgtc gtacttatcg ccggtccctg tggcgtcgaa
     1921 agcgagcagc aaattttaga aatagcgcag gccgtcaaag aagccggggc gcacattttg
     1981 cgcggaggtg cgtttaagcc ccgcacttcg ccttatagct tccaaggatt aggtgaacgc
     2041 ggtctcgaat tgcttgcttt ggcacgcgaa aaaaccggta tgccgatcgt gactgaagtg
     2101 gtgacgccca atcaggtcga actcgtcgct aaatatgccg atatgctaca gatcggcgca
     2161 cgcaattcgc ttaattattc acttctgcaa gaagtcggca aaactaataa gcctgtattg
     2221 ctcaaacgcg gcatgatgtc gcatctggaa gaatatatga tgtcggccga atacatttta
     2281 gccaacggta attataacgt agtattgtgt gaacgcggaa ttcgtacgtt tgaaacgtcc
     2341 acacgcaata cgatggatat tgctgcaatt cccgtactta agtcatggac gcatcttccg
     2401 gtgatagccg atccgagtca tgccaccggc aaacgttcgc tggtacaacc ggtagccaaa
     2461 gcggccgtcg cggccggagc ggacggcctt atgatcgagg tgcaccatca tcccgaacat
     2521 gccttatcgg acggtccgca agccgtactg cctgatgaat acgccgcatt ggtccgccaa
     2581 ttgcgacaaa tcagctccgt actcggacgc tcgatcgcct aaacgaaatt tatttcataa
     2641 ccgtttgttc atgaaagtat ccaccgtgac gacgcgttat tcctatcttg aagaatcttc
     2701 attgtcgagt gaaaaactcg tggacggcca tctgctcaag gcctggcgcg atgtcgtaca
     2761 gttgcccgac ggtaaaacag gcattcgcga atatattcgc caccccgggg cggtgatcat
     2821 gttgccgttg tttgaaaatg gtgataccat gcttattcgt caatttcgat ttcctccaaa
     2881 aaaaatattt tgggagttgc cggccggtaa attagacgga ccggaagatc cgctggtggc
     2941 agcgcagcgc gaactgatcg aagaaacagg atggtcggct tcgcactgga cgcgcgtcgg
     3001 ttcgttttat ccctgcatcg gatacagcga tgaacgaatg atcttgtaca tcgccgaaga
     3061 cctcacaccg gcggagtccg cgcacgatga cgacgaattt atcgaacgat ttcgtatgcc
     3121 gcgcggcgat gcattgcaaa aagtatggtc cggggaaata gatgatatga aaaccatagc
     3181 cgcgcttttg ctggccgaac atacgctacg caacaactct aaaacgaggt gatgtatggg
     3241 tacatttgac cgcgatgaca aagacggttg gcgcgaacgg gaaggtgatg aagaagaacc
     3301 ttggaaaaaa gcacacgaaa actggaaaaa cgattcccgc ttatccgatg atgttccggc
     3361 tacccgcgaa acatctccgt tttggacaga agaacgctgg gaacatttta ttcaggaaac
     3421 ggacaaagac gggcttcagg ttatcaagta ctacgaacgg tactggaatc atcaggatcg
     3481 tgatgtcatg gtcgaaaacg ccatggcgct tcattacgta cgggaagcgt tcaaatatat
     3541 tcatccggaa aatcataaat ctttttacgt gcattatttt ttagaaccgg aatgtgcttt
     3601 gatgaaactg ccggtgcaaa gcagcggcca ttttcttgaa gcgaagttac gaacggtgtc
     3661 tgatattcag gcgtaccgat tatcgcgtga tttcgcgcgg tttgcatacc gttggctaaa
     3721 agatctgccg gatgaattgc gaatggaaaa agtctcggaa agttttactt ccaatatact
     3781 caatgtttgc accaaacttt ccggcggcca tgtgatggga taccagccgt ggacgatggg
     3841 cggtcaaatc gtattgacca aacgcgcatt acgttcgatc aatcgctgta tggaaattct
     3901 ttttaccgtc aaggatcagt gggcgctgga ttannnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnctcatcc aaagccgatg tggcttcatc caaaagtaaa atttccggtt
     4081 tacgcaaaac aatacgtgcc aaagctagcc gctgacgctc accgccggac atggtccatc
     4141 ctttttctcc gatgatggta tccaagccat caggtaatgt tttaatccat gtgtccagtt
     4201 gaacatgcgt gagcgcttcg atcacatgtt catcgctgac atgaatttga tcgtacacga
     4261 cattgtctct taccgtcgca tgcatgatca gcggttcctg tgtcatgagt ccgaaaagtt
     4321 ttgcacgtgt agcgtgcgaa atttgtgtta ctggaatgcc gtcaagcgtg atacagccgc
     4381 gttggggtgc atacaacccg atgatcaaat ccaaaatagt gcttttaccg gcgccggttt
     4441 tgcctgtgat aataacgcac tcgcccctac gaatagttat atttactttg tttaaaattt
     4501 cggtggtgcg atgtgcaaaa caagtttctt ccaaacaaag tgcgttcgtg aatgaagcga
     4561 tatctttcgt cggtgatgtt tcttcggacg acgccatcgg tttgtttttg tattcgtccc
     4621 acaacgtacg ggcttcggat aaaaacatac gtccgcgtgc gatatgccgc gccggatcaa
     4681 tgatcgaaaa cgcggcggcg ataaataata caaaaccgcc gggcccatac ataaattcgc
     4741 cttccatcgt ccatgagcca atcgtcaata ggagcattac acccacgctc acacccgcca
     4801 tttctaccgt ggccagtacg atcgcttgca accggtcacg acgggttgcc gcatcgctcc
     4861 gacggctttc cagtgatgaa aaacgcttcg cttcgtaccc ggcgctgtga aatagtcgaa
     4921 tcaatttgaa agcttgcagc ttatgccaga catccgactg gatttcggat tcgcgctcgt
     4981 gtaaaaaaag cgcccatcgc gctgtgtatt tttgcaaacg atgttgcaat aacgtaatgc
     5041 ccggaatcaa tacaagactt aataacgtga tcttccagct gagtataaaa agcaccgccg
     5101 aatagagtac aagtaaagga aaatgctgac aaagagaaaa aatccattgc atccatgcgc
     5161 gtaccatagt ttc
//
LOCUS       DAOQGF010000175         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_8328_length_5160_cov_7.242311, whole genome shotgun sequence.
ACCESSION   DAOQGF010000175 DAOQGF010000000
VERSION     DAOQGF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8328_length_5160_cov_7.242311"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..868
                     /locus_tag="PK937_15045"
     CDS             107..868
                     /locus_tag="PK937_15045"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNH33941.1"
                     /translation="MNLKQIAVQREIVYRSNARIVFGIMRRFFSLQFDDLISRLRSQQ
                     RAPLTVNIDDLFNDWNKWDAMLSGRVSPQVIQSVRAGFEQMKASIKISGTFNPNDVYI
                     QNAIFESMKRVTSINENTRNRVTAVITEYIQTGKGIDELVIKLQSEVLPLYQKSRART
                     IATTTTTTSMNAGSHAMMQRFKDDIDYQQWFSQKDDKVRESFNYDHVAADGQRVQIGQ
                     PFIVSGEALRYPGDPSGSLGNIINCRCFAIPIKKK"
     gene            1175..2206
                     /locus_tag="PK937_15050"
     CDS             1175..2206
                     /locus_tag="PK937_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33942.1"
                     /translation="MIDEIYNKQEQYFREHLNNEDRRGARSFGFSRMADDKASDGEPV
                     FIVNTPSVDGHRSVISPRGLDFSNKYYKNNPIWVFDHDNRLVVGQADGSGWIKYSKAD
                     KSEIKSKIRYASTDFPQEVRTLVVENILRMASIWFDPIVQFFDQAARSEYEKDYADLG
                     LKAPDYMNWYIKESGLLHNSIVAIGSNLDAYAQARGSYSPEMQRAIEFAIMQEVIPSL
                     RADIAALRAELVTLRDNGAKNLTESIKEKEQAIADKVADMETERKAGDGESPLCQSDK
                     AVSIPDPQKVIEATIARDVNDARKLSVDENTMKEFNAWKRKRAELDSLRTSIDKTFKT
                     IKYYQGQVE"
     gene            2206..3492
                     /locus_tag="PK937_15055"
     CDS             2206..3492
                     /locus_tag="PK937_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNH33943.1"
                     /translation="MEDKKVEVPEADLDAFRKFQAEQAEAARKEKEKNELSDIQVLRD
                     KQDAMQMMLEKALKYNELVASNRSISKLAIDNVKDEKETRAKKWDVLMRAFLAGDLPK
                     YNEVAKELSSVTRGFVTTNSTAQYNVPSDVRTDLLYTTETFGILGRDTMNYTTQSNSV
                     QLPFLNTSLSVGFVAQAAAPISQSGTATQVTLSIAAMKAILGTFSEETLDDAPSLYNS
                     WVQILGKGIMQKRDDTLFNGDGTASYGLFRGIFQEVAATGGITEKSIAGGALSTITID
                     DLRGIIAAVPVSARAGAKFYFHRTFEPFLYGMKDLNNRPIFIEGNTANPSTLLGYPVE
                     LSEVCPDVSGVVGLGTKFLAFGNLKNSCHFVDRQQMTMKIGYEGTVDGEAMFAEGKSA
                     LRVMERWALKVYTMPQPFLGAASNVRRLGLVTLATA"
     gene            3686..4156
                     /locus_tag="PK937_15060"
     CDS             3686..4156
                     /locus_tag="PK937_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33944.1"
                     /translation="MKKILILMFVLCADLFAQANTIPIVWARFSAPNTQITTISGTDS
                     LFSNSFGGQIDSLHLVCGLPANDSLRVKITLQSGINNVWENVAVDFDSVVSVGTQQLK
                     RVDLKFRAGNLTNASMRLKIRAYNTAAVTTANGVSTGAPDLSSVYWKVYTRLRP"
     gene            4240..4896
                     /locus_tag="PK937_15065"
     CDS             4240..4896
                     /locus_tag="PK937_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33945.1"
                     /translation="MALSSYAIFDDVSDAYGVLGISGDAPESQKSFIEYCINAATEDL
                     EQLLNRNLISRAYTDESHTIESELQYDREGREYNIAPTILYLRHYPITAITSIKFDNV
                     ALTDITEGETTGIWWSASDLANSGRLVYESGWPCGERRIKINYTAGYTVSNLPYIYRR
                     VGNELIQWHYSKSGYGDKSLQMAQIQGTISPGTGNFRTHDEMMTYFGKQLTPYRSING
                     "
     gene            4889..>5160
                     /locus_tag="PK937_15070"
     CDS             4889..>5160
                     /locus_tag="PK937_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33946.1"
                     /translation="MAKIVTNSDKLSRDFERVKAETVKMYAALAQNVFDQMAIDSTGN
                     MRILGGRSTGRRLLTKIGNTKPLGAVLTSRSGRIVASLNNKVFRNK"
BASE COUNT         1553 a          998 c         1288 g         1321 t
ORIGIN      
        1 atccgaacga cgaatccgac ccgatgaata aatattactt aacggctggt cttacgccga
       61 tagatgaggt gtcgttattc ggcgggcaag acaatagtac tgggctatga atttaaaaca
      121 aatagcggtt cagcgcgaaa tagtttatcg gtcaaacgcc cgtattgttt tcgggattat
      181 gaggcggttt ttttctttac agttcgatga tttaatatcg cgcttgcgct cccaacaacg
      241 cgccccgcta acggtgaata tagatgactt attcaacgac tggaacaaat gggatgctat
      301 gctatcgggg cgtgtatcgc cgcaagtcat acaaagcgtt agagctgggt tcgagcaaat
      361 gaaagcgtca attaaaataa gtggcacgtt taatccgaat gacgtatata ttcaaaacgc
      421 catatttgaa agcatgaagc gcgtcacgtc aataaacgaa aacacgcgaa accgtgtaac
      481 ggcggtaata acggaataca tacaaacagg caaggggata gatgagcttg tgattaaatt
      541 acaaagcgag gtcttgccgt tatatcaaaa gtcacgcgcc cgcacgatag ccaccaccac
      601 aacaacaaca tccatgaacg cgggaagcca tgcgatgatg caacgcttca aggatgatat
      661 agattatcag caatggtttt cgcagaaaga tgacaaggtc agggagagct ttaactatga
      721 ccatgtagcg gcggacggtc agcgcgtgca gatagggcaa ccgttcatcg tgagcggcga
      781 agctttgcgc tatcccggcg acccgtctgg cagcttaggt aacataataa actgtaggtg
      841 ctttgctata ccaataaaga aaaagtaaaa aacacttgac atttaaaatt taattttgta
      901 taatggccaa cgtgaggttg tatgatttat tccctgagct tgagaacgac attgtcaagc
      961 taattttaga cggtaagtac gcggaattta aaataaagtt tgaagcgggt aagcccgtaa
     1021 taagtgaaac gacggagaaa aaaaggtaca cggaaaagaa gcagtaatta aatagtttaa
     1081 gcttatctaa acaatagagg gctttttgtc ttagttgcgc cgcacagggg tggcgtgatt
     1141 agacgaaaag ccctttttta tttggtggaa taagatgatt gacgagattt acaataaaca
     1201 agagcagtat tttagagaac acttgaacaa tgaggaccgc cgcggggcgc gttctttcgg
     1261 gttttcgcgt atggcagatg ataaagccag tgacggggag cctgtgttta ttgtaaacac
     1321 gccgtctgtt gacgggcata gatcggtgat ttctccgcgc ggcttagact tttctaacaa
     1381 atattacaaa aataatccga tatgggtgtt cgatcatgac aatcgcttgg ttgtagggca
     1441 agctgacggc agtggatgga taaaatattc taaagccgat aagtctgaaa ttaaatccaa
     1501 gatacgttat gcgtcaaccg attttcctca agaggtgcgc acgcttgttg ttgagaatat
     1561 tttgcgtatg gcttcaatat ggtttgatcc aatagttcag ttttttgatc aagccgctcg
     1621 ttctgaatat gaaaaagatt atgccgactt aggattaaaa gcacctgatt acatgaactg
     1681 gtacataaag gaatcgggtc tattgcataa tagtattgta gcgataggtt caaatttaga
     1741 tgcatacgcg caggcgcggg gaagttattc gcctgaaatg caacgggcta tagagttcgc
     1801 aattatgcaa gaggtcatcc catcattgcg agccgatata gctgcattgc gtgccgaact
     1861 tgtcacattg cgtgacaatg gggctaagaa tttaacggaa tcaataaaag aaaaagagca
     1921 agcgatagcg gataaggtgg cggatatgga gacagaacgc aaggcgggag acggtgaaag
     1981 cccgttatgc caaagcgata aagctgttag tattcctgac cctcaaaagg ttatcgaagc
     2041 aacaatagcg cgtgacgtaa atgatgcgcg taaattatcg gtagatgaga acacgatgaa
     2101 agagtttaat gcgtggaaac gaaaacgcgc ggagcttgat tcgttgagaa cgtctattga
     2161 taaaacattc aaaacaatta agtattatca aggacaggtg gaataatgga agacaaaaaa
     2221 gtagaagtgc cggaagccga tttagatgcg ttccgtaaat ttcaggcaga acaggcggaa
     2281 gcggcgcgta aagaaaaaga aaaaaacgag ttatccgata ttcaggtatt gcgcgataag
     2341 caagacgcga tgcagatgat gttggaaaaa gctttgaaat acaatgaact tgtggcgagc
     2401 aaccgaagca taagcaagct tgcgattgac aatgtaaaag atgaaaaaga gacccgcgct
     2461 aaaaaatggg atgtacttat gcgggcattt ttggctggcg atttgccgaa atacaacgag
     2521 gtagcaaagg aattgtcatc cgtaacgcgc ggatttgtta caaccaatag caccgcgcaa
     2581 tataatgtgc cgtctgacgt gcgcaccgat ttgttataca cgacggaaac tttcggtatt
     2641 cttggccgag acacgatgaa ttatacaacg caaagcaatt cggttcagtt gccttttttg
     2701 aatacttcgc tatcggtcgg gttcgtcgcc caagccgccg cgcctatatc tcaaagcggt
     2761 acggctactc aggttacgtt aagcattgcc gcaatgaaag caattcttgg tacatttagc
     2821 gaagaaacgc ttgacgacgc accgtcttta tacaatagct gggtgcaaat tctcggtaaa
     2881 ggtatcatgc aaaaacgcga tgataccttg tttaatggcg atggaacggc gtcttatggt
     2941 ttgttcaggg gtattttcca agaggttgcc gctaccggcg gtatcaccga aaaatccatc
     3001 gccggcggtg cgctttcgac aattacgatt gacgacttgc gcggaataat cgccgccgtt
     3061 cctgtttcgg cacgcgctgg ggctaaattc tattttcacc gcacgttcga gccgttcttg
     3121 tacgggatga aagatttgaa taatcgcccg atatttatag agggcaatac cgcgaatccc
     3181 tctacgttat tgggttaccc agtcgaactt agtgaagtat gtcccgatgt ttcgggtgtc
     3241 gtgggtctcg gaactaaatt ccttgctttc ggtaatctta aaaattcttg ccatttcgtt
     3301 gatcgccaac agatgaccat gaagatcgga tatgaaggca cggtggacgg cgaggctatg
     3361 tttgccgaag gtaaatcggc gttgcgtgtg atggaacgct gggctttaaa ggtctataca
     3421 atgccccaac cgttcttggg cgcggcaagt aatgtgcgcc gcttgggtct tgtaactttg
     3481 gctacagcgt aatgttgtgc atagttaaat accctcgctt ggactacaaa ggcaaaagct
     3541 ataatcaagg cgaaaaggtg gacattgaag aaattcatgt ccaccatgcc ttgaaaaacg
     3601 agcttgtaga gaagtatgag ccgagcgata caacaagaat gaatgtaaca gaaaatacaa
     3661 cgagaccaaa aaaaggaaaa atgaaatgaa aaaaatattg attttaatgt ttgtactttg
     3721 cgctgattta tttgcgcagg cgaatacgat acctattgta tgggcgcggt tttctgctcc
     3781 aaacacgcaa attactacta tcagcggcac ggattctttg ttctcaaatt cgtttggcgg
     3841 gcaaattgat tctttgcatt tagtatgcgg attgccggca aatgattctt tgcgtgttaa
     3901 aattaccttg cagtccggaa taaataatgt ttgggaaaat gttgccgttg actttgattc
     3961 cgtggtatcg gtagggacgc aacagcttaa gcgcgttgat ttaaagtttc gcgctggtaa
     4021 tctaacgaat gcttcgatgc gtctaaaaat tcgcgcatat aatactgcgg cggtaactac
     4081 ggcaaacggc gtttccaccg gtgcgcctga tttgtcaagc gtttattgga aagtctatac
     4141 acgtcttcgc ccttaatttt ctacgcggtt atttaacgct gtagaacatg gttaaagggg
     4201 cttgaaaaat agcccctttt ataaagtggg attaaaaatt tggcactttc aagctatgca
     4261 atatttgatg atgtaagcga tgcctatggc gttttgggca tatcgggcga cgcgccggaa
     4321 tcccaaaaat cgtttatcga atattgcata aacgcggcta cagaggattt agagcagctt
     4381 ttaaaccgca atcttatttc acgcgcatac accgacgaat cgcataccat agaatcggaa
     4441 ttgcaatatg atagggaagg gcgggagtat aatatcgcgc ctaccatttt atatttgcgt
     4501 cattatccga taaccgcaat cacatctatc aagtttgaca acgtagcact taccgatatt
     4561 acggagggag aaacaactgg gatttggtgg agcgcatcgg atttggctaa tagcggtcgt
     4621 ttggtttatg aaagcggttg gccgtgcggt gaacgtagga taaaaataaa ttacacggcg
     4681 ggttataccg tatcgaatct gccttatatt tatcgccgcg tagggaatga attgattcaa
     4741 tggcattatt ccaaatcggg ctatggtgat aagtcgctac aaatggcgca aatacaaggc
     4801 actatttcgc ccggtacggg gaatttcaga acacacgatg agatgatgac gtacttcggc
     4861 aaacaactta cgccctatag gtctatcaat ggctaagata gtaacaaatt cggataagct
     4921 atcgcgtgat tttgagcgcg taaaagcgga gacggtgaag atgtacgccg cgcttgcgca
     4981 aaacgtgttc gatcaaatgg ctattgatag cacgggtaac atgcggatat taggcggacg
     5041 ctcaacggga aggcgattgt tgacaaaaat aggcaacact aagccgctgg gcgcggtgct
     5101 aacatcgcgt agtggccgca tagtcgcatc gttaaataat aaagttttta gaaacaaaaa
//
LOCUS       DAOQGF010000176         5146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_8365_length_5146_cov_5.614025, whole genome shotgun sequence.
ACCESSION   DAOQGF010000176 DAOQGF010000000
VERSION     DAOQGF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8365_length_5146_cov_5.614025"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2293)
                     /locus_tag="PK937_15075"
     CDS             complement(<1..2293)
                     /locus_tag="PK937_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH33947.1"
                     /translation="MSRRVFSVIGFLFCVAILPVYGQIKSVAVMRFENRSSFPENEWI
                     GVGFSETIATKLSLVETITMVERVKLNEVLAANKKTDLLSDSQKVEAEEARTIIGVEY
                     LLIGSVQTLGSYKDLNSKIKISARIISTKSAAILGDAAFTLDGTVKDLFDLQSKIAAK
                     FCAAIGVSATASAYNFEDAMNLTAQQSFGEGMNAFYKHDYKKAIDFFRQALNSNEGSF
                     YASAHTMEGTARQKQIESAAAGDQDKLKKEYLEQFRTDASQAAPAFYDLGTAYKATEN
                     WSEAIKAYSEFLKYAGTNAKVVLWKKVIKGGCFTLGDNKVYAGTDGPPAMTTYDLESG
                     NLLSELKLKGEFVCRDLMYANGHVIWDGWSRIMSFDPTKPDPLWTYKENFDERSPLLI
                     GNGVLAWLSEKTIYGVDLQTGKKLWSTSSETKKPSGILLNQKTAYVNNDGNFERDAYV
                     SKYDFTTKKASRAGTEKVYAIGPQYSLYLSSNYVKGIENETGNQSWSVWDTDISPKKQ
                     QPHFQMAVADSQRFYLVSRDADITSQYSESGLSKKFIVGLDAATGNALWYHAVDGFLS
                     SQMVSSHGLLFVATRYGNAEKYQDKKRINIYALDAVTGEKVWNHEVPEQVTKLEVKDD
                     YLLAVTERNSDTDKKYVYTFRITNMKKPLLLYSDLDARFQHAYCLLKSGDLAAAEMEA
                     RELLELKNDYLPAYYLMSLVHRVRGNAAEELAALHEYYLRGNFEVVKERVYELSGIKQ
                     IYRFPKHEKAYLAKKGSSIFLKII"
     gene            complement(2340..5135)
                     /locus_tag="PK937_15080"
     CDS             complement(2340..5135)
                     /locus_tag="PK937_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNH33948.1"
                     /translation="MMNQQWRRGFFLLLIPLLSAFSQNQAVLRSVAVLRFELRSASTE
                     NDWLGFGFAETLTSKLGMVDGLMVMERTKLHELLNIAGKSEPLDETQVSAGEEYARII
                     GAEYLLIGSVQVVGSYKNTSTAIKVNARIIKTSSAEITGNMAFSVSGTVQEIFELQTQ
                     LASRFCEVIGAPAGLAELRYEDAQSIDAQQWYGRGLQAFYESNYAQAITCFRRAINQN
                     EGVFYAAAHTMEGMARQKQIDRANAADKQTYKQQYLDQFRKDAGEASPAFYDLGIAHQ
                     AAGNYTESIKNFRTFLAYLDRTRQLVVQNPFTGEETSAPEESYPYVFETANGLRVSTE
                     EGKEILYYPDPEINSDAKILIHQDTLFVMANSIFAFALKPGRFLWKYPQGIRFDYTMV
                     AESGRLYIVGFDQIQIFVGGSGERMAYWRMPALTDLNYIRIARGIFWSGYDSIAYNLN
                     ALLSNRPSHSEIDAWFQIGRSYEDAGQTDSALCYYDDVLKARGEYIEALDHKSSILNQ
                     TGKTSEAVKVALDAVRYGVPCRSRDLLYACTGLSGYTSVPGWMSHVETADTMAYIGVG
                     TPGFVGKAFSKIYGIHLRRMQSDWAFDFQCGSYMPSFTMNKRTLFAYKPVENKYALIA
                     IARLDGQLLWQNDLRASYIPSEYYDEFIRPAASDNVVVSVPGSVSGWNASDGKRLWQI
                     TQSAETFDENPDGWVLPAEMNEGDFNMSYLGGTPLAINHRFYVSSPKTLWCISEEGKK
                     LWQTSTAHHKLSLRYENSMISVQDFIASDTAKRFYDPMYGRRLASAPDREFHRVHKDE
                     THSAFDGKHVYTLEEYANGITVKKQGSGVPAHSEVFQLTNYSTDMGKWITLADDEIWV
                     LLGYLNQDGTDYGGVVVLDAPTLRCKWKLDIGKNVVHMFATKDAYFIFCSRGDIIRMS
                     KTKMNELLARGQLWWKK"
BASE COUNT         1381 a         1292 c         1013 g         1460 t
ORIGIN      
        1 agatgatttt aagaaaaatg gacgaacctt ttttagccag atatgctttt tcatgtttgg
       61 ggaagcgata gatctgtttg atgccggaca attcatacac ccgctccttt acaacttcaa
      121 aattccctcg taaataatac tcatgcagtg cggctaattc ttccgccgca ttaccgcgca
      181 cccgatgtac caatgacatc aggtaatacg cagggagata atcgtttttc agttcaagta
      241 actcacgcgc ttccatttct gccgccgcca agtcgccgct cttcaggaga caataggcat
      301 gttgaaaccg tgcatccaga tcgctataca ataaaagcgg tttcttcata ttggttatgc
      361 gaaacgtata aacatacttt ttatccgtgt ctgaatttct ttcggtaacc gccagcaaat
      421 aatcgtcttt gacctccagt tttgtaacct gttctggcac ttcatgattc cacacttttt
      481 ctccagtcac ggcatcgagc gcgtagatgt ttattcgctt cttatcctga tatttctccg
      541 cattaccgta tcgcgtggcc acaaaaagca acccgtgcga actaaccatc tgcgaggata
      601 aaaatccgtc aacggcatgg taccataacg cattgcccgt tgcggcatct aggcctacga
      661 taaacttttt actcaatccc gattcgctgt actgagatgt tatgtcggca tcccggctaa
      721 caagataaaa acgttggctg tcggccaccg ccatttggaa atgcggttgt tgctttttgg
      781 gagaaatgtc ggtgtcccac acactccatg attggttacc ggtttcgttt tcaatccctt
      841 tgacatagtt actggacaga tacaggctat attgcggtcc tatcgcataa actttttcgg
      901 taccggcgcg cgatgctttt ttggtcgtaa aatcatattt acttacatag gcatcacgct
      961 cgaaattacc atcattattg acatacgcgg ttttttgatt gagcaaaatg ccgcttggct
     1021 ttttagtctc cgagctcgtg gaccataatt ttttaccggt ttgtaaatca acgccataaa
     1081 ttgtcttttc agaaagccat gctaaaacac catttccgat caataaaggc gagcgttcat
     1141 caaaattttc tttgtaggtc cacaacggat ccggtttagt cggatcaaaa ctcataatac
     1201 ggctccaccc gtcccagatg acatggccgt tagcatacat caaatctctg caaacaaatt
     1261 cacccttcag ttttaactcc gataaaagat tacccgactc taaatcatag gtggtcatag
     1321 ccggagggcc atccgtgccg gcgtacactt tattgtcacc caaagtaaaa catccgcctt
     1381 ttataacttt tttccacaac acgactttgg cgttagtgcc ggcatatttt aagaattcag
     1441 aatatgcttt gatggcttcg ctccagtttt ccgtcgcctt ataagccgtt ccaaggtcgt
     1501 aaaatgccgg tgccgcttgc gatgcatcgg tgcgaaattg ttccaaatat tcttttttca
     1561 gtttgtcttg atcacccgca gcagcggatt cgatctgctt ttgtcgtgcc gtaccttcca
     1621 tggtatgcgc tgaggcataa aaacttcctt cgttagaatt caacgcctgg cgaaaaaagt
     1681 cgatcgcttt tttataatcg tgcttataaa acgcattcat accttctccg aacgactgtt
     1741 gagccgtgag attcatagca tcttcgaagt tgtacgcaga ggccgtagcg ctgacaccta
     1801 tagcagcaca gaattttgca gcgattttag attgtaaatc aaataaatcc tttaccgtgc
     1861 cgtccaatgt aaaagctgca tcgcccaaga tcgccgccga cttcgtgcta atgatacgcg
     1921 cactgatctt aatctttgaa ttcagatctt tatatgaacc cagcgtttgc actgaaccga
     1981 taagcagata ttcaacgccg ataatagtac gcgcttcttc cgcttccact ttttgtgaat
     2041 cggacaacaa atctgttttt ttattcgccg caagcacttc attcaatttc acacgctcaa
     2101 ccatcgtgat ggtttcgacc aacgacaact tggttgcaat ggtttctgaa aacccaacgc
     2161 cgatccactc gttctcagga aacgatgacc ggttttcaaa acgcatgacc gctaccgatt
     2221 tgatttgtcc ataaaccggc aggatggcca cacaaaacaa gaaccctata acagaaaaaa
     2281 ctcgcctgct cataatattc tcgcctttca ttttagtgtg aatatttaaa aattgaacct
     2341 tattttttcc accacaattg accacgcgct aaaagttcgt tcattttcgt tttagacatg
     2401 cgaataatat cgccgcgcga acaaaaaata aaatacgcat cctttgttgc aaacatgtgt
     2461 acaacatttt tcccgatatc caatttccat ttacatcgca acgtaggagc atctaaaacc
     2521 accacaccgc cgtaatctgt accatcctga ttgagataac cgagtagcac ccaaatttcg
     2581 tcgtctgcta atgtaatcca ttttcccatg tccgtgctat aatttgtcaa ctgaaaaact
     2641 tctgagtgag ccggcacacc cgatccttgt tttttaacag ttattccatt ggcatattct
     2701 tcaagggtgt aaacatgctt tccgtcaaaa gcggaatgtg tttcatcttt atgcacccga
     2761 tgaaattcac gatccggcgc agatgcaaga cgacgcccat acatgggatc ataaaacctt
     2821 ttggccgtat ctgaagcgat aaaatcttgt acgctaatca tgctattttc ataacgaagc
     2881 gatagtttgt gatgcgctgt cgaagtttgc catagttttt ttccttcttc cgaaatgcac
     2941 catagcgttt tgggtgatga cacataaaag cggtgattga tcgcaagcgg cgttccaccc
     3001 agatacgaca tattaaaatc tccctcattc atctccgccg gcaaaaccca accgtccggg
     3061 ttctcatcga atgtttccgc ggactgtgtt atttgccata gcctttttcc gtccgatgca
     3121 ttccaacccg aaacggaacc cggcacagac acgaccacgt tatcacttgc cgccggacga
     3181 ataaattcat cataatattc cgacggaata tatgacgccc ggagatcgtt ttgccataac
     3241 agttgcccgt ctaaacgagc gatcgcgatt agtgcgtact tattctctac gggtttgtac
     3301 gcaaaaagtg tgcgcttatt catagtgaat gaaggcatat aactgccgca ttggaaatca
     3361 aatgcccaat ccgattgcat tcggcgcagg tggataccgt atatttttga aaaagccttt
     3421 ccgacaaatc ccggcgtacc gacaccgata tacgccatag tgtccgccgt ttccacatga
     3481 ctcatccatc ccggtacaga tgtatatccg gaaaggccgg tacatgcata taataaatca
     3541 cggcttcggc atggcacacc gtaacgaaca gcatccaaag ctactttcac cgcttcggat
     3601 gttttgccgg tttggttgag tatagaggat ttatgatcaa gcgcctcgat gtattcccca
     3661 cgtgctttta aaacatcgtc ataatagcac aaagccgaat ccgtctgacc cgcgtcttca
     3721 taacttcggc ctatctgaaa ccatgcatct atttcggaat gcgatggacg gttggacaat
     3781 aaggcattta gattgtacgc aatgctgtca tatccggacc aaaaaatccc tcgtgcgatg
     3841 cgtatataat ttaagtccgt caacgccggc atacgccaat acgccatgcg ctcacccgaa
     3901 ccaccaacaa aaatctgtat ctgatcgaaa ccaacgatat acaacctccc gctttccgcg
     3961 accatcgtgt aatcaaaacg tatgccttgc ggatatttcc ataaaaaacg accaggcttg
     4021 agtgcaaaag caaaaatgga attggccatg acaaataatg tatcctgatg aatcagaatc
     4081 ttggcgtcgc tgtttatttc tggatcagga taatatagaa tttctttccc ttcttcggtg
     4141 ctcacccgta gtccgttagc cgtttcaaaa acgtagggat acgattcctc aggagcggaa
     4201 gtttcttcac ccgtaaaagg gttttggaca acaagttgac gagtgcgatc aagataggcg
     4261 agaaatgttc ggaaattttt tatggactcc gtgtagttac ccgccgcctg atgagcgatg
     4321 cccagatcat aaaaagcagg cgacgcttca ccggcatctt ttcgaaactg atccagatac
     4381 tgttgtttgt aggtttgttt atctgcagca ttcgcgcgat ctatttgttt ctgtcgtgcc
     4441 attccttcca tcgtatgtgc ggcggcataa aatacacctt cgttttgatt aatcgcacgg
     4501 cgaaagcatg ttatcgcctg ggcataattc gattcgtaaa aggcttggag cccgcgccca
     4561 taccattgtt gtgcatcaat gctttgcgcg tcttcatatc gtaactcggc taatccggcc
     4621 ggcgctccga taacttcaca aaatcgagaa gccagttggg tttgcaattc aaaaatttcc
     4681 tgtaccgttc ctgaaactga aaatgccata tttccggtga tttctgccga acttgttttg
     4741 ataatacgcg cattgacttt gatcgctgtt gacgtgtttt tataactgcc gaccacttgc
     4801 acggatccga tcagcaaata ttctgcgccg atgatccgcg catattcctc acctgcactc
     4861 acttgcgttt cgtccaaggg ctcagattta ccggcgatgt tgagtaattc atgcaacttt
     4921 gtccgctcca tcaccatcag accatccacc atacccaatt tggacgtcag agtttccgca
     4981 aaaccaaatc ctaaccaatc attctccgtt gaagccgagc gaagttcaaa acgaagtaca
     5041 gctaccgacc tgagcacggc ttgattttgt gaaaaagccg aaaggagagg gataagtaaa
     5101 agaaaaaaac ctctgcgcca ttgttgattc atcatcaaga cctatc
//
LOCUS       DAOQGF010000177         5130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_8409_length_5130_cov_7.059507, whole genome shotgun sequence.
ACCESSION   DAOQGF010000177 DAOQGF010000000
VERSION     DAOQGF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8409_length_5130_cov_7.059507"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK937_15085"
     CDS             complement(<1..695)
                     /locus_tag="PK937_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNH33949.1"
                     /translation="MKPTRRIVLFIFAITAVLLLGMVLQEKGKITLTKDMIAQAEKLF
                     GLQFSEAKRDSMLESLQEQLGNYEAMRKIKLDNSVAPALQFNPIPVGFKIDQDKKSFK
                     PARLDNVIPPPALEDLAYYSVSELAWLIKNKKVTSELLTKMFLDRLRRYGPKLENVIT
                     ITEGLAIKQARKADAEIAQGKYRGPLHGIPYGVKDLLATVGYKTTWGSVPYKDQLIDE
                     DATVVRRLEEAGAV"
     gene            complement(1245..1979)
                     /locus_tag="PK937_15090"
     CDS             complement(1245..1979)
                     /locus_tag="PK937_15090"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNH33950.1"
                     /translation="MNIGKKIGICAHSYEGGALCFLSTCKEGAQILGAHYHPTIILSA
                     VPMGLSMPAYETNNHDEVGKYLREGVDRVAKAGADFFICPDNTAHIILEKIISTLPIP
                     GLHIADVVCKEIIDNGWKRVGLLGTNWTMTGPVYSQALKEKNLIKQIPDDSTREEIHK
                     AIFEELCQGIFRKETIAMFQNAIENLKNKGAECVILGCTEIPLIITPENSALPIVDST
                     RLLAKYAVRHALSENNSGDVGWIRII"
     gene            complement(2278..3855)
                     /locus_tag="PK937_15095"
     CDS             complement(2278..3855)
                     /locus_tag="PK937_15095"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH33951.1"
                     /translation="MTYTRVIIFVFLQIISALAWTSVAQTKNMSADTTRLKHHVMTMS
                     HDSMRGRRSGYEGEQKAASYIENQFQNIGLSPFIGTSYLQPFLLPRGRINAASYVIIN
                     EDTLRYLTDFLTYEVPDKRTDIESDMVFLGYGIDGPGKSYDAYGGNDIHGKIVVALQP
                     SLIDRARLGEPLNYDHMQLASWARTYGARGLILLENNPGSNKKRFNSASYSEVYRPFS
                     DNFFWISIQNGYAFIQRYFNITQERIEPVMRLTAPVRINTRIDGYWLKSANVIGKLEG
                     TDLANEYIVVTAHYDHIGIGKKINGDSICNGMLDNASGVATMLEAARMLYSSQTKPRR
                     SVLFVATGAEEMGFIGSQYFADHLPIPQSQIVANINLDITGYYYKSEEPYVTLLGAEY
                     NDMGDFIEDAADESGVRVGSNTIPSFFYRSDAVVFARAGIPSIHIACGLPISEMKSGM
                     EYFMNYYHQTTDELYKAPVSFKIMAGQTETVARSIIKIADHDKRPIMTSELDSEKWMG
                     WREAWGGFRMWVGGFFE"
     gene            complement(3930..4535)
                     /locus_tag="PK937_15100"
     CDS             complement(3930..4535)
                     /locus_tag="PK937_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNH33952.1"
                     /translation="MKRFFWLSLIVMVFRISLFAQGYNVGDKAIDFRLKNIDGKTVSL
                     ADYKDAKGFIVTFTCNHCPYAKAYEERIMALDKKYRPLGYPVLAINPNDPVGYPEDSY
                     ENMIARAKEKGYTFPYLVDETQNIARAYGATRTPHMYVLQKTSSGLEVAYIGAIDENT
                     ENASAAKNKYVEWAVDALLKGQKPSTTFTKAIGCTIKWKKS"
     gene            complement(4539..5036)
                     /locus_tag="PK937_15105"
     CDS             complement(4539..5036)
                     /locus_tag="PK937_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNH33953.1"
                     /translation="MHRNFLLLWLITWSVMGQVEKVSLPELERRLMLNKDSVLVVNFW
                     ATWCSPCIEELPLFDSLYQNAANYKSEKKSVTVMLVSLDFASQLESKVKPFVIKQRLK
                     APVILLDESDANVYINRMHHDWSGAIPATLLIRPSTGERKLIERKFESYDDLKQSIFH
                     FIERK"
BASE COUNT         1483 a         1142 c          991 g         1514 t
ORIGIN      
        1 accgcacccg cctcttctaa tctacgaaca accgtcgcat cttcgtcaat gagttgatct
       61 ttatatggta cagagcccca cgtcgtctta taacctaccg tcgccaggag atctttgacg
      121 ccgtaaggaa tgccgtgcaa tgggccgcga tatttgcctt gagcaatttc ggcgtcggct
      181 ttgcgtgctt gtttgatagc taatccttcg gtgatcgtaa tcacattttc aagtttgggc
      241 ccgtaacggc gcaaacgatc taaaaacatt ttggtcaata attcagacgt aacctttttg
      301 tttttgatta accaagccaa ttcactgacc gaataatacg cgagatcctc caatgccggc
      361 ggcggtatca cattgtccag gcgcgccggt ttgaatgatt ttttatcctg atctatttta
      421 aatccgacgg ggataggatt gaactgcaac gccggcgcaa cggaattatc cagtttgatt
      481 tttcgcatcg cttcgtagtt acccagttgt tcctgcagag actccagcat cgaatcacgc
      541 ttggcctcgc tgaactgcaa tccgaaaagt ttctcggctt gagcaatcat atctttggtt
      601 agggttattt tacccttttc ctgcaatacc ataccaagca aaagcacagc ggttattgcg
      661 aaaataaata aaacgatacg gcgggtcggt ttcatactaa atcctcatta gatttggata
      721 gaaaaatata cgaataccgt agataaaaca aagcctctta ttgtaaacga ataatttatt
      781 ggcaataatg attgttttta caaagccggt aagctttttt acactttata cgccgcgcta
      841 aaactaacag aaatataact atcaaattcc ttccgtggtg ctcagttccc cgatttgtca
      901 tcatatttaa aattaattat actatacatc aatatggcag ggttaattcg gcaaaacata
      961 tggaagtaaa tcagagcata gcataataat atgtggctaa aaaaaagcag gcctcaagtc
     1021 aaacgcaact cggtcaatta ttcaaataaa ctggagattc tatttaatac actctatcat
     1081 aggtacattt cctatcggtt atcgctattc tcaaagaatt gtgaccgaaa caatatcctt
     1141 ggcatttcta aagattacgg cctactattg acgatgatcg gcgtgatgat aacgtgaacc
     1201 ggagttacgg aactacttca gataaaacga catttaaata tttctcaaat aattctgatc
     1261 catccgacat ctccggagtt gttttcggat aatgcatgcc ttacagcata tttagcaagt
     1321 agtcgagttg aatcaacgat aggaagagcc gagttttccg gggtaatgat taatggaatt
     1381 tccgtgcagc ctaatattac gcattcggcg cctttgtttt ttaagttctc aatggcattc
     1441 tgaaacattg caatggtttc ttttctgaag attccctgac acaactcttc aaatatagct
     1501 ttatgaattt cttctcttgt agaatcatcg ggaatctgtt tgattaaatt tttttcttta
     1561 agggcttgtg aataaacagg accggtcata gtccaattcg tacctaaaag tccgactctt
     1621 ttccaaccat tatcaataat ttctttacag acaacatcgg caatatgtaa tcccggaata
     1681 ggaagcgtcg aaataatctt ttctaggata atgtgcgcgg tgttatctgg acaaatgaaa
     1741 aaatctgcac ccgctttagc aacacgatca accccttctc ttaaatattt gcccacttca
     1801 tcatgattgt tggtctcata ggctggcata ctcaaaccca ttggaacggc gctaagtatg
     1861 atcgtaggat gataatgagc acccagtatc tgcgctcctt ctttacaggt agaaagaaag
     1921 cataaagctc cgccttcata gctatgagcg caaatcccga ttttttttcc aatattcatt
     1981 actgttcaat cctttttaaa aaaattaata aattacaaac ttgagcaaaa tcatatcgct
     2041 tagattgtta cgatcaatca tgatacatga gcaacaacaa tccaaaacca taaaaccatg
     2101 gtctgatgca ttgagttcaa ggtttatctc ttcagttaaa attaatagca ctattaacta
     2161 aaaggcggaa ttgattgcca atcaaacgaa tgtaaattaa catattaaat taaaatgtat
     2221 aatataataa tataccttga aaaaaagaaa aatatcgggc caactgcgat ctgttaatta
     2281 ttcgaaaaac cctccaaccc acatgcggaa tccgccccac gcctcgcgcc agcccatcca
     2341 tttttccgaa tccaattccg aagtcataat aggccgttta tcatgatcgg ctattttgat
     2401 gattgagcga gccacggttt ctgtttgacc ggccatgatt ttaaaactca cgggcgcttt
     2461 gtataattca tccgttgttt gatgatagta attcataaag tattccatac cgcttttcat
     2521 ttcgctgatc ggaagaccgc acgcaatgtg gatggacgga atacctgctc tagcgaacac
     2581 aaccgcatcg ctgcgataaa aaaacgaggg gattgtattg gaccccacac gcacaccgga
     2641 ctcatcagcc gcatcttcga tgaaatcgcc catatcatta tattccgcac cgagtaaagt
     2701 gacataaggt tcttcggatt tataataata ccccgtgatg tccaaattga tattggcaac
     2761 aatttgggat tggggtatcg gcagatgatc ggcaaaatac tgcgatccga taaatcccat
     2821 ttcctccgcg ccggttgcaa caaaaagcac actacgccgg ggttttgttt gcgatgaata
     2881 caacattcgc gccgcttcca acatagtcgc cactccggac gcattgtcta acataccgtt
     2941 acagatggag tctccgttta tttttttacc aatgccgata tggtcatagt gcgctgttac
     3001 gacgatatat tcattggcca gatccgtacc ttctaatttg ccgatcacat tggcgctttt
     3061 aagccaatat ccgtctattc gcgtattaat tctgaccggt gcggtaagcc tcatcacagg
     3121 ctcaatacgt tcttgtgtta tgttgaagta acgttgaatg aaggcgtatc cgttttgtat
     3181 agatatccaa aaaaaattat cagaaaaagg gcgatacacc tccgaatacg atgcagaatt
     3241 gaatcgtttt ttatttgacc ccgggttgtt ttccagcaaa ataagtcccc gtgcaccata
     3301 ggttcttgcc caagatgcca actgcatgtg atcataattt aaaggttcac caagacgtgc
     3361 acgatcaatc agcgacggct ggagcgcaac gacgatttta ccgtgaatat catttccacc
     3421 gtatgcatcg tacgattttc ccggcccatc aatgccatat cccagaaaaa ccatgtcact
     3481 ctcaatgtct gttcttttgt ccggcacttc ataggtcaaa aaatcggtaa gataacgcag
     3541 cgtatcctca ttgataataa catacgatgc ggcgttgata cgcccgcgcg gcagaagaaa
     3601 cggttgtaag tagctcgtac cgataaacgg acttaaaccg atgttttgga attgattttc
     3661 aatataggat gctgcttttt gttcgccctc ataaccggat ctgcgtccac gcatgctgtc
     3721 gtgcgacatg gtcattacgt gatgtttcag tcgtgtcgta tcggctgaca tatttttggt
     3781 ttgagctacg gatgtccaag ctaatgctga gataatttgt aaaaatacaa aaatgatgac
     3841 cctcgtatac gtcatgtatt ctccgttttt aaaaaatcaa cccgcttgtt tttgaagcgg
     3901 gttgtgtaaa aacattttat tgagcaagat tacgatttct tccacttgat cgtacagccg
     3961 atagctttgg tgaatgtcgt actcggcttt tggcccttga gtagagcatc cacggcccac
     4021 tcgacgtatt tattttttgc cgccgatgcg ttttcagtgt tttcatcaat agcgccgata
     4081 taagccactt ctaatccgga ggatgttttt tggagaacat acatgtgcgg agtacgcgtt
     4141 gcgccatacg cacgagctat gttttgcgtt tcatctacca gatatggaaa cgtataacct
     4201 ttttctttgg cacgggcgat catattttca tacgaatctt caggataccc taccggatca
     4261 ttaggattga tcgcaagcac cggataacca agcggtctgt attttttatc cagcgccata
     4321 atgcgttctt cataagcttt agcgtatggg caatgattgc acgtaaaagt aacgataaaa
     4381 cctttagcgt ctttataatc ggcaagcgag actgttttcc cgtctatatt ctttaggcgg
     4441 aaatctatcg ctttatcgcc cacgttataa ccctgagcga acagggaaat ccgaaaaacc
     4501 atcacgatga gggataacca aaaaaaacgt ttcatggttc atttcctttc aataaaatga
     4561 aatatgcttt gttttagatc gtcataggat tcaaattttc gctcgatgag tttacgttca
     4621 ccggtggaag ggcgaatcaa cagcgtagcc ggtatcgcac cgctccagtc gtggtgcatc
     4681 cgattgatat acacattggc atccgattcg tccagaagaa tcaccggcgc tttcaatcgc
     4741 tgtttgataa caaatggctt taccttggac tccagttgtg aagcaaaatc caagctgact
     4801 aacataaccg ttacgctttt cttctcagat ttataattcg cagcgttttg gtatagcgaa
     4861 tcaaacagcg gcaattcctc aatacaagga ctgcaccacg tagcccaaaa attgaccaca
     4921 agaacactgt ctttgttaag cattaatcgt cgctcaagtt caggcagtga cactttttcc
     4981 acttgcccca ttacagacca agttatcaac cacaataaaa gaaaattccg atgcattttt
     5041 tatcagggat ttaataaaac caggcaagac acaggatgtg ccttgcctga caatttatta
     5101 tacaggagct ggtgttttta cgttagccgt
//
LOCUS       DAOQGF010000178         5064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_8563_length_5064_cov_5.710321, whole genome shotgun sequence.
ACCESSION   DAOQGF010000178 DAOQGF010000000
VERSION     DAOQGF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_5064_cov_5.710321"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK937_15110"
     CDS             complement(<1..438)
                     /locus_tag="PK937_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33954.1"
                     /translation="MTNIEVIEGYGRENYGSFVLKKVYQKNVSTGLIIGITLHIAGIM
                     TYYISTVVFKEEQIRMVRITDINELQSAPPLQEQTAAPKVKVEVQEVIKPVAGIPIVV
                     PDAEAKPDMTIQTQEEIKTDIAAPTSFGSDGGEVKVDISGTIGD"
     gene            complement(435..1013)
                     /locus_tag="PK937_15115"
     CDS             complement(435..1013)
                     /locus_tag="PK937_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH33955.1"
                     /translation="MAEVGGAQPRSHGKGKKKKKARTGVRLDMTPMVDVAFLLLTFFM
                     LTTAFRLPQTMEISIPEDDKPENNVKIAESNIMQIRIDLDNNIFWNQGNEKPQKATMK
                     ELREIIIERSEKNIANGKGDTIKDKFKLIVLIKIDKRAKYEHMVNIVDELNLAMFDLN
                     QKNNLTEANRLSPRFAFAPFSEQDQKEVAKAQ"
     gene            complement(1032..1559)
                     /locus_tag="PK937_15120"
     CDS             complement(1032..1559)
                     /locus_tag="PK937_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNH33956.1"
                     /translation="MAKHKKHRVGVKLDMTPMVDVAFLLLTFFMLTTTFRPPEEISVT
                     VPDSHSQLKLPSANVITLSVAKDPIILKKGTQNEENTGTRIWMDVDAQVVRGKIFGAD
                     YAQSAGKAVKLDELQNLITKARLENLAIRGASGAMRVVLKADKDADYGVVSEIIDILQ
                     KSKITTVNFITNLEK"
     gene            complement(1582..2337)
                     /locus_tag="PK937_15125"
     CDS             complement(1582..2337)
                     /locus_tag="PK937_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNH33957.1"
                     /translation="MKQSVFMSILITTAFIVSYLVFEFIFGAEGDKYGLHYIYAGGPL
                     VVILMVLSIMVVTFIFERIFSLRKANGKDSLPFFLKKIQKSLMDGNVETSIEVCDHQK
                     GSCANIIKMGLEAFQRGSKMGMNMEKRVTETKRAIEEATSLEVPLLERNLIALSTIAS
                     ISTMVGLLGTTLGMIRAFKALAQSGAPDAVSLSLGISEALINTAGGLGAAIVAIVAYN
                     YFVNKVDTYTYLIDEASYNVVEILTTTHGDDKN"
     gene            complement(2449..2784)
                     /locus_tag="PK937_15130"
     CDS             complement(2449..2784)
                     /locus_tag="PK937_15130"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNH33958.1"
                     /translation="MKVKTDPSGVVVIAPKGRLTGGSETDELKQEIERVVAEGITKCV
                     IELSETIYLNSTALGLLVSAHKKFIDRGGKLKLSGVNKNIDNILVITKLSLVFEVFDT
                     EAEALASFK"
     gene            complement(2854..4485)
                     /locus_tag="PK937_15135"
     CDS             complement(2854..4485)
                     /locus_tag="PK937_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33959.1"
                     /translation="MRSFLKKTLVITLMLISANQVVVAQGPQIKQALLLINKGQNKEA
                     VEYLLQIVNEYPKTAELRYLLAMALDKNGQTDMAIEEAKSVVTMKPKYKDNRRLLMDL
                     YIKKENFKNAMEECQFLLKESKKDIGLKLNLCEIYLGLENYKDASTELAKLEVEKPND
                     NLVLTLLGDAYAKQRVNSLAIQYYNKALGVNPNAVNTKLKLAKLYFRETQYNEALKEY
                     LDVVRMDTTNAEALLRIGIIYNAAGKSDVNQYGRAIGFLQRYVGIAPNDYAGYFYLGK
                     SWHALRSWQNAVTNFESAIKLDDSARVKEESTALLSESYRGLGVNYLKDKNYDAMTPA
                     IKKSIELNPASDSIAFEIGYSFYGEKNYATAASWFDISAQSKTASEPKIANSLFLSGV
                     SRYNAATTKADSMAALAPIEKSLALRPTLNNYLNFAQYNESVDQPAKARAAYEKAVEM
                     DSNSVTAQLKLGILLFNKFKNDTEPAIKAFKKVGQLDEKGSYKMSHYFLGLCYLNAKK
                     KPDAREAFKKYLEVDPTGNFSKQAKTYVTQLAGAR"
     gene            complement(4625..>5064)
                     /locus_tag="PK937_15140"
     CDS             complement(4625..>5064)
                     /locus_tag="PK937_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNH33960.1"
                     /translation="GNYRVLHEMVLDKNPLSPNAVVLSGDSANSVVPKILDFVASTPG
                     AIGYVSTAWVGKTSADWESRSGKLKVIRVSGEDFHKAVEPIQGYVYRGDYPLRRVLYI
                     IHRQPYIGLAAGFTAFLTGNDGQKICLANNLVPGMNPIRLKHE"
BASE COUNT         1328 a         1147 c         1049 g         1540 t
ORIGIN      
        1 atcgccgatc gttccggaaa tatccacctt cacttcacca ccgtcgcttc cgaaactggt
       61 cggtgcagcg atatctgttt tgatttcttc ttgtgtctgt atggtcatat ccggcttggc
      121 ttcggcatcg ggcaccacga tgggtatacc ggccaccggt ttgatcactt cctgtacttc
      181 gacttttact ttcggcgcgg ccgtttgttc ttgcagcggc ggagccgatt gtaattcgtt
      241 gatatccgta atacggacca tacggatttg ttcttcttta aagactaccg tactgatgta
      301 gtacgtcata ataccggcta tatgcaatgt aataccgata atcaaacctg tgctgacatt
      361 tttctgatat acctttttga gaacaaatga accgtagttc tcccggccgt agccttcgat
      421 gacttcgatg ttggtcattg ggctttcgca acctccttct ggtcttgctc gctaaaggga
      481 gcaaacgcaa atcgcggtga gagacgattt gcttcggtta ggttattctt ttgattcagg
      541 tcaaacatgg caagattgag ttcgtccacg atattgacca tgtgttcgta ttttgcccgt
      601 ttgtcaattt tgatgagaac gatcaattta aatttatctt tgatcgtatc ccctttgccg
      661 ttggctatat ttttttcact gcgttcgatg atgatttcac gcaattcctt catcgtagcc
      721 ttttgcggct tttcgttacc ctgattccaa aaaatattat tatccaaatc tatacggatt
      781 tgcataatat tggactcggc tattttgacg ttattttcag gcttatcatc ttcagggatg
      841 ctgatttcca tagtctgcgg cagacggaat gctgtcgtaa gcatgaaaaa tgtcagcaga
      901 aggaacgcca catccaccat cggcgtcata tccaaacgca caccggtgcg ggctttcttt
      961 ttcttcttgc ccttgccgtg ggatcggggt tgtgcaccac ctacttcagc catgttagta
     1021 ctccgctaaa attatttctc aagattggtg ataaagttca ccgtcgtgat cttggatttt
     1081 tgtaaaatgt caatgatttc gcttacgaca ccgtaatcgg catctttgtc cgctttgagc
     1141 accacacgca tagcaccgga agcgccacgg atagccagat tttctaaacg cgctttggta
     1201 ataaggttct ggagttcgtc cagtttaacc gctttacctg cactttgtgc ataatctgca
     1261 ccgaaaatct ttccgcgtac gacctgcgca tccacatcca tccaaatgcg tgtgccggta
     1321 ttttcttcgt tttgtgtgcc cttttttaga ataatcggat ctttggcgac cgataaggta
     1381 atcacattag ccgaaggcag cttcagctgt gaatgtgaat ccggtacggt aaccgaaatt
     1441 tcctccggtg gacgaaaggt cgtcgtaagc atgaagaacg tcagcagaag aaacgcgacg
     1501 tccaccatgg gcgtcatgtc aagctttacg ccgacacgat gttttttatg ttttgccata
     1561 gtatgttacc acgccttcaa attagttctt atcgtcaccg tgagttgtgg tcagaatttc
     1621 aactacattg tagctggctt cgtcaatcag gtaagtatag gtgtccactt tattgacgaa
     1681 gtagttatat gctacaatag ctacgatcgc agcaccgaga ccaccggccg tattgataag
     1741 tgcttcggag ataccgagcg agagggatac ggcatccgga gcaccggatt gagcaagcgc
     1801 tttgaatgcg cggatcatac cgagggtcgt accaagcaat cccaccatgg tggaaatcga
     1861 agcgatcgtt gaaagcgcga tcaggttacg ttcgagcaac ggtacttcta aagacgtggc
     1921 ttcttcgata gcacgttttg tttcggtcac gcgtttttcc atgttcatac ccatttttga
     1981 accgcgttga aacgcttcca aacccatttt aataatgttt gcgcaggaac ctttttgatg
     2041 atcgcaaacc tcaatcgaag tttccacgtt gccatccatc aggcttttct gaattttctt
     2101 gaggaaaaaa ggaaggctat ctttgccgtt agctttacgc aacgaaaaaa tacgttcaaa
     2161 aataaatgtc acaaccatga tggacagaac catcaaaata actacgaggg gaccgcccgc
     2221 gtaaatatag tgaaggccat atttgtcacc ttcggcacca aaaataaact cgaacaccag
     2281 ataggacacg ataaatgccg tggtgatcaa aatagacatg aatactgact gcttcataag
     2341 gaaggtcctc ctgaaaaata ggatttattt aatgtatgac taagatttgc cttaaacagg
     2401 ttcgggaaag atacgagtct atcccgaagc atacaagtta tttttatgtt acttaaaact
     2461 cgcaagcgct tcggcttcgg tatcaaacac ttcaaacacc agtgatagtt tggtgatgac
     2521 gagtatatta tcaatatttt tattaacgcc ggataatttc aattttccgc cgcggtcaat
     2581 aaatttttta tgagcggaca ccaaaagccc taaagccgta ctattcagat aaatcgtttc
     2641 ggataattcg attacgcact tggtaatccc ttccgctaca acgcgttcga tttcctgttt
     2701 tagttcgtcc gtctcggaac ctccggtcag acggcctttg ggggcgatca ccaccacgcc
     2761 ggacggatca gttttgactt tcatcgcaat ctccttttat aatatttcaa cttttcaata
     2821 gtaccaatca acacaatgct tgggcgttaa aaactagcgt gcgccggcaa gctgtgtaac
     2881 atatgtttta gcttgtttag aaaaattacc cgtcggatct acttctaaat attttttgaa
     2941 tgcttcacgt gcatccggtt ttttcttcgc attgagataa cacaaaccta agaaataatg
     3001 cgacatttta taggaacctt tttcatcaag ctggccgacc tttttaaatg ccttgatcgc
     3061 aggttccgta tcgtttttaa atttattaaa caacaaaatt ccaagcttga gttgtgccgt
     3121 tacactgttg ctgtccattt cgacggcttt ttcgtaagct gcacgcgctt tagcgggctg
     3181 atctaccgat tcgttgtact gtgcaaagtt aagataatta ttcaatgtgg gacggagcgc
     3241 taatgatttc tcaatcggcg ccaatgcagc catgctgtcg gctttggtcg ttgccgcatt
     3301 ataacgcgat acgcctgata agaataggct gttggcaatt ttgggctcgc tcgcagtttt
     3361 gctttgcgca gaaatatcaa accatgatgc ggccgtcgca taattttttt cgccgtaaaa
     3421 actatatccg atttcaaacg cgatcgaatc actggccgga ttaagttcga ttgacttttt
     3481 gatcgccggg gtcattgcat catagttttt gtctttgaga taattcacac cgagaccgcg
     3541 gtaggattcc gataaaagag cagtcgattc ttccttaact cgagcggaat cgtccaattt
     3601 gatcgctgat tcgaaattgg taacagcatt ttgccagcta cgaagggcgt gccagctttt
     3661 accgagataa aagtatccag cataatcatt gggcgctata ccgacatatc gttggagaaa
     3721 accgatagcc cggccatatt ggttgacatc ggattttccg gcggcgttat atataatacc
     3781 gatacgcaaa agtgcttcgg cattggtcgt gtccatacgc acaacatcga gatattcttt
     3841 gagcgcttca ttgtactgcg tttcacgaaa atacaactta gccagtttga gtttcgtgtt
     3901 tactgcatta ggattaacgc ctagggcttt attataatat tgtatagcca gtgaattgac
     3961 acgttgctta gcatacgcat cacctaataa cgtcagtacc aaattatcat tgggtttttc
     4021 tacttcgagc ttagccaact ccgtgctggc atctttataa ttttcaagac cgaggtatat
     4081 ttcgcacaga ttaagcttga ggccgatatc ttttttggat tccttaagaa ggaactggca
     4141 ctcctccatc gcatttttga agttttcttt tttaatataa agatccatca aaagccgacg
     4201 gttatcttta tatttaggct tcatcgtcac gacgcttttg gcttcttcga tagccatatc
     4261 cgtttgaccg tttttatcta atgccatggc caatagatag cgcaattctg cggttttagg
     4321 atattcgttt actatctgaa gcagatattc cacagcttct ttattttgcc ctttattgat
     4381 cagtaataat gcttgtttga tttgtggacc ttgtgcaaca acaacttggt tagctgaaat
     4441 taacatcaaa gttataacta acgttttctt caagaaacta cgcatactaa ggtacttcct
     4501 ctttgttaaa ataagatcgg atctaacaat aaattataga caagatatag attcgtcaaa
     4561 aaccggattg ggaccaaaaa tgacggaaaa atctatgcga ttttagaaaa aaaacaagct
     4621 ttttttactc atgcttaagc cggatgggat tcatccccgg aacaaggtta ttggccaaac
     4681 atattttttg tccgtcattg cctgtcaaaa atgccgtaaa accggccgcc aacccgatat
     4741 acggctgacg gtgaataata tataataccc gtcgcaacgg ataatcgccg cgataaacat
     4801 acccttgtat gggctctact gctttgtgaa agtcttcacc cgacacacga ataactttta
     4861 atttgccgga acgcgattcc caatccgcgc tggttttacc cacccaagcc gtgctcacat
     4921 atcctatggc gcccggcgtt gatgctacaa aatccaaaat tttgggtaca acactattgg
     4981 ccgagtcgcc ggacaaaacg acagcattgg gtgacaacgg atttttgtcc agtaccattt
     5041 cgtgcaaaac tctataatta ccgg
//
LOCUS       DAOQGF010000179         4892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_9034_length_4892_cov_5.486872, whole genome shotgun sequence.
ACCESSION   DAOQGF010000179 DAOQGF010000000
VERSION     DAOQGF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9034_length_4892_cov_5.486872"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..102
                     /locus_tag="PK937_15145"
     tRNA            31..102
                     /locus_tag="PK937_15145"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Gly,seq:ccc)
     gene            235..1215
                     /locus_tag="PK937_15150"
     CDS             235..1215
                     /locus_tag="PK937_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH33961.1"
                     /translation="MSSISPEERYDRAEQYVAKARLFYLQGDMEEALESYSQALQYDS
                     SHTEALLGSAEIWTQQKRYNDILIALRNLPSKNPRTAYLRGQAYFATSRYNEALVDLL
                     SVLKHPAYGLLALRMTISSYARNGAYAQAVDLLDKELPSHDPATVRDLILERERLHDA
                     LLMAEPMRGFEEKKLAVLEKQSTITRSDLAFLAVTLLSLKKDSAGLAFRDVPAQDTMA
                     LYYRTAIRCGFLEILPDGKFYPDHIIRRRNLAHYMYRLLSAPDVKLTASPWSDIDPRD
                     PIIAAALWAHQNKVLTPQTGTQFGVDKPVTGLEVKKALERLALLRVEFFK"
     gene            1323..2033
                     /locus_tag="PK937_15155"
                     /pseudo
     CDS             1323..2033
                     /locus_tag="PK937_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
     gene            2022..3410
                     /locus_tag="PK937_15160"
     CDS             2022..3410
                     /locus_tag="PK937_15160"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNH33962.1"
                     /translation="MERIASGEPLRAETQADVLTQPATTVAETIKRIRIVRHQWNKAL
                     DYITDLDFVVQNTFGRWSVRDVIAHFIGWERILIERLGHVKNNNAEGVAVFTDEGVDE
                     VNTQWVTRFKESDRSEVMDEWNAAQSDLQHIIQSLTDSMLASQARGRTVSEIIAGSSF
                     AHEYEHLQKVQSWLQTFQTASTSTVGVPRVRSDVKPSSSIPNPPFWGDRVVEDISLTD
                     IFPFINEVALFRGQWQFTRGKMSGSEYQSFVDAKVRPVFDAWKKRALEEKLLIPKVVY
                     GYFPCQSDWNDLVIYENDHTTEKLRFTFPRQSDNHHYCISDFFAPKESGKMDVIGCML
                     VTVGQQASDFSAQLFESDNYADYLYFHGLSVETAEALAEYWHRQMRLELGIVSGDHSD
                     TKKLFQQSYQGSRYSFGYPACPHLEDQSKLFTLLDPKRIGITLTDEYHLVPEQSTSAI
                     IVHHPEAKYFNI"
     gene            3423..4727
                     /locus_tag="PK937_15165"
     CDS             3423..4727
                     /locus_tag="PK937_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013620573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33963.1"
                     /translation="MKRNLMLGITAALFIASCTKTELPTMNTIAEHYVRLILKIGLHD
                     GDYVDAYYGPDSLKPKSDTALLKTEYALTALLTECDQLLAQLAQIKPDPKQRMDVLRH
                     TYLVKQLTAARTKIEMLNGKKYSFDEESRLLYDAVAPTYDSIHFRAILTELEQKMPGH
                     GNLTDRLEEFRKAFIIPKDKLDAVFQAAIKEGRRRTQQYIPLPEKENFTVEYVNNKPW
                     SGYNWYKGGAYSLIQVNTDLPIFIDRAVDLACHEGYPGHHVYNALLEKTLVRDRGWIE
                     FTVYPLFSPQSLIAEGSANYGIEMALPGAERMKFEKEVLFPLAGIDAKRVDEYYAVQA
                     LVGKISYAGNEAARGFINGTMTREQAIDWMATYGMYARERAEQRLRFIERYRSYVINY
                     NYGLDLVRGYIEKNGGTSDKPETRWKLFEKLLSEPTMPSALQ"
     gene            4724..>4892
                     /locus_tag="PK937_15170"
     CDS             4724..>4892
                     /locus_tag="PK937_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNH33964.1"
                     /translation="MKKDSSYPLIWATVMKIPKGKVAAYGQIAYIAGLGRQPRLVGYA
                     LHNIPEGMDIPW"
BASE COUNT         1341 a         1051 c         1222 g         1278 t
ORIGIN      
        1 gtctgagaag taataagaca gtatttaaat gcgagagtaa ttcagtggta gaatgccagc
       61 ttcccaagct ggacgtcgcg ggttcgaccc ccgtctctcg ctcagaagat cattaggaaa
      121 cgtcattcgg acggagttcg gaatggcgtt ttttgttata accagagacc tagagtgaga
      181 cgcgaactgt tttttagttt agtatttttg cttagttgta ccggttgcac atcggtgtca
      241 tctatatctc ctgaagagcg gtatgatcga gccgaacaat atgtagcgaa ggcgcgttta
      301 ttttatttac aaggcgatat ggaagaagca ttggaatcat actcacaagc tttgcagtac
      361 gattcatcac ataccgaagc actgttgggt tcagccgaaa tatggacgca gcaaaagcgt
      421 tacaacgaca ttctaatcgc tcttcgaaat ttaccgtcta aaaacccgcg cacggcgtat
      481 ttacgcgggc aagcctactt cgcaacatcc agatataatg aagcgctggt tgatctttta
      541 tcggttttaa aacatccggc ttacgggtta ttggctttac gcatgacgat cagttcgtat
      601 gcccgcaatg gtgcttatgc gcaggctgtt gatttactcg acaaagaatt gccttcgcat
      661 gatccggcga ccgttcgcga tttgatcttg gaacgggaac gtttgcatga tgcgcttttg
      721 atggccgaac cgatgcgggg gtttgaggaa aaaaaattag ccgttctgga aaaacaatcg
      781 acgattacac gaagtgattt ggcgtttcta gcggtaacgt tattgagctt gaaaaaagat
      841 agcgccggtt tggcatttcg tgacgtaccg gctcaggaca cgatggcgtt gtattaccga
      901 accgcgatac ggtgcggctt tttagaaata ttaccggacg gaaaatttta tccggaccac
      961 atcatacgac gccgtaacct tgcgcattat atgtatcgtt tactaagtgc gccggacgtc
     1021 aagcttaccg cgtccccatg gtccgatatt gatccgcgtg atccgattat tgcagcagct
     1081 ctgtgggctc atcaaaataa agtgcttaca ccacaaactg gaacccaatt tggagtggat
     1141 aagcccgtaa ccggattgga agttaaaaaa gcgttagagc gtctggcgct tttgcgggtc
     1201 gaatttttta agtagaattg ataacagaaa cttattgttc cgcgtagttt gtgcttatat
     1261 attgtttggt gcatttaaaa taaccgaggg ttcctgtgtc tgattcgtca caaaaagtcg
     1321 tgtatgatcc gttgattgaa atcatcaatc actttgcttc taaaaaaaat gaagtcagcg
     1381 tgtctaaatc aacaaccgcc gccacggtcg aagaacggtt gaaaaaccgg attatcgaag
     1441 gtgaaaaaaa aggtttggat gatgatctca aattggccat gcagaattat aaaccgcttg
     1501 aaattatcaa taccattctc ctcgaaggaa tgaaagtcgt aggcgatttg ttcggcaaag
     1561 gcgagatgca gctgcctttt gttttgcaat cggcggaagt gatgaaaacc gccgtggctt
     1621 acctggaacc atttatggaa aaatcatccg gttctcaaaa aggtacgatg gtgcttgcta
     1681 cggtcaaagg cgatgtgcac gatatcggaa aaaatctcgt agatatcatt ctgaccaata
     1741 acggttataa agtcatcaat ctcggtatta aagtaccggt agaaactatg ctgcaatcgc
     1801 tgcatgagca caaagccgac gcgatcggga tgagtgggct tttggtaaag tcaactctga
     1861 tcatgaaaga aaatcttgaa gtgatggccg aacgcggcat acaggttccc gttttgctgg
     1921 gcggtgcagc gttgactcgt cgttacgtag aggaagattt acgttccgtg tatgaggcgc
     1981 cgttgtttta ttgcgaagat gcattttcag gattacgcgt tatggagcgc attgccagcg
     2041 gtgaaccgct gcgggcggaa acgcaggcgg acgtgctcac gcaacccgca actacagtag
     2101 ctgaaacgat caaacgtatt cgtatcgtac gtcatcagtg gaataaggcc ttagactata
     2161 tcaccgattt ggatttcgtc gtacaaaaca catttggtcg ttggtccgtg cgtgatgtga
     2221 tcgctcactt tatcgggtgg gaaagaatac tgatcgaacg attgggtcac gtaaaaaaca
     2281 acaatgcgga gggcgtggct gtatttacgg atgaaggcgt ggacgaagtc aatacgcagt
     2341 gggtgacccg tttcaaagaa agcgaccgat ccgaagtgat ggacgaatgg aatgcggcac
     2401 aatccgattt acaacacatc atccaatcgc tgacggattc gatgttagcc tcacaagcgc
     2461 gggggcgaac cgtatctgaa atcatcgcgg gctcttcatt tgcacatgaa tacgagcatt
     2521 tgcagaaagt gcaatcctgg cttcaaactt tccaaactgc gtcgacgagt accgtcggtg
     2581 taccgcgtgt tcgttccgat gtgaagccct catcgtctat tccgaatccg cctttttggg
     2641 gggatcgcgt agtcgaagat atttcgttaa cggacatttt tccgtttatc aacgaagtcg
     2701 ctttatttcg cggtcagtgg cagtttacca gaggaaaaat gagcggcagt gaatatcaga
     2761 gtttcgtaga tgcgaaagta cgccccgtat ttgatgcgtg gaaaaaacgc gctttggaag
     2821 aaaaacttct tattcccaaa gtcgtttacg ggtattttcc ttgccaatcg gattggaatg
     2881 atctggttat ttatgaaaac gaccatacta ccgaaaaatt acgttttaca tttccgcgtc
     2941 agtcggataa tcatcactac tgtatttctg atttttttgc gcctaaagaa tccggcaaaa
     3001 tggacgtgat cggatgtatg ctggtcacgg tcggacaaca agcgtcagat ttttcagcgc
     3061 aacttttcga atcggataat tacgccgatt atctttattt tcacggcctc agcgtggaaa
     3121 cggccgaagc cttggctgaa tattggcatc gccaaatgcg actggagtta ggcattgtga
     3181 gcggggatca tagcgacacc aaaaaactct ttcagcaatc gtatcagggt tcacgttata
     3241 gttttgggta tccggcatgc cctcatctgg aagatcaaag taaattgttt acactgctgg
     3301 accctaaacg cataggcatc acgctgaccg atgagtatca tcttgtaccc gaacaatcta
     3361 ccagtgcgat catcgttcat catccagaag ccaaatattt taatatataa cgaggaatac
     3421 atttgaaacg caatttaatg cttggcatca ccgcggcttt gtttattgcc agctgtacta
     3481 aaacagagtt accaactatg aatacgatag cagaacatta tgtgcgtctg atccttaaaa
     3541 taggactgca cgatggcgat tatgtggatg cgtattacgg gccggacagc ctcaaaccga
     3601 aatcggatac ggctttactt aaaaccgaat atgcgttaac cgctctgctc acggagtgtg
     3661 atcaacttct tgcgcaactg gctcaaataa aacccgatcc gaagcaacgc atggacgtat
     3721 tacgtcatac gtatttggtt aaacaactga cggcggcgcg tacgaaaatc gaaatgctca
     3781 atggaaagaa atattcgttt gatgaagaat cgcgtttgct ttatgatgcc gtcgcaccga
     3841 cgtacgattc gatccatttc cgagcgatcc tcacggagct ggagcaaaaa atgccgggcc
     3901 atggcaatct cacggatcgt ctcgaagaat ttcgcaaggc ttttattatt cccaaagata
     3961 aactggatgc ggtcttccag gcggctatca aagaaggacg ccgccgcact cagcagtata
     4021 tcccgctgcc ggaaaaagaa aattttacgg tcgagtatgt aaacaataaa ccctggagcg
     4081 gatacaattg gtataaaggc ggggcttaca gtttgattca ggtgaatacc gacttgccga
     4141 tttttattga tcgtgccgta gatctggcct gtcacgaagg gtatcctggt catcatgttt
     4201 acaatgcgtt acttgaaaag acactcgtac gggatcgggg ttggatcgaa tttacggtct
     4261 atcctttgtt cagtccgcag tcgctgattg ccgaaggcag cgccaactat ggtattgaga
     4321 tggccttgcc cggtgcggag cgcatgaaat ttgaaaaaga agtattattt ccgctcgccg
     4381 ggatagatgc caagcgcgtt gacgaatact atgccgtgca ggcgcttgtt ggaaaaataa
     4441 gttatgcggg caatgaagcg gcacgcggtt ttatcaacgg tacaatgaca cgcgagcaag
     4501 ccattgactg gatggccacg tacggtatgt acgcacggga gcgcgcagag caacgtttgc
     4561 gttttatcga acgctaccgc agttatgtga ttaattataa ttacggcctt gatttggttc
     4621 gcggctatat tgaaaagaat ggcggcacct cggacaaacc ggaaacgcgg tggaaattgt
     4681 ttgagaagct actgtcagaa cccaccatgc cgtcggcgct ccaatgaaaa aagacagttc
     4741 atacccgctt atttgggcaa cggtgatgaa aattcctaaa ggcaaagtcg cagcttacgg
     4801 tcaaattgct tatatcgcgg gactgggccg acagccaaga ttggtggggt atgcgctgca
     4861 taatattccc gaaggcatgg atataccgtg gc
//
LOCUS       DAOQGF010000180         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_9229_length_4826_cov_4.986376, whole genome shotgun sequence.
ACCESSION   DAOQGF010000180 DAOQGF010000000
VERSION     DAOQGF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9229_length_4826_cov_4.986376"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK937_15175"
     CDS             complement(<1..235)
                     /locus_tag="PK937_15175"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNH33965.1"
                     /translation="MLQSIFISIADTLVDASFYLLIGFILAGILRGLFTVEMLGKHLG
                     DSSWRSVWKAALIGVPLPLCSCSVVPVGISLKKN"
     gene            383..456
                     /locus_tag="PK937_15180"
     tRNA            383..456
                     /locus_tag="PK937_15180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:417..419,aa:Arg,seq:acg)
     gene            506..1192
                     /locus_tag="PK937_15185"
     CDS             506..1192
                     /locus_tag="PK937_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH33966.1"
                     /translation="MHRILVIIPAFNAHSTINGILSVLNQFKDRSRPLDIDLDTLVVD
                     DGSDPRLETHLNSGYGHIITHTLNKGKGAALKSGFKFASDEKYDYVVTMDADGQHDPN
                     RLLEMVSFGLRGDYDLVIGERNISLKSMSLGRFLSNKITTKIIRRMSGLDIKDSQCGF
                     RFIKIDLLEKFELHTDNFDTESEFLLRASKYKIQYANFPIPTINNLSHSHINHLRDTV
                     RFIRLLLKSI"
     gene            complement(1261..>4826)
                     /locus_tag="PK937_15190"
     CDS             complement(1261..>4826)
                     /locus_tag="PK937_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH33967.1"
                     /translation="LSSTQWGIGNKLYIKSGTFTIGTNNIEIGGSLRNFGTWTTPANT
                     ITFTGTTATDTIGGSNAIAFRNLTLTKAASQNLRLETPVTVKNSLTMSNDGNIILTGS
                     NALTIDTSATVSAGSGSFSASRCIIQTGALGGPQIIKNLAAAPVVASFTFPIGYGSYY
                     LPAVMTFGTRTGTGNIAVRAVSGANPNIIVPSKALDQYWTVDVTSFTNVKLLQSFYYN
                     NAEAQGTETNYVPRYWAGSSPWLTPSNGFESSAGDVFGHQDSMTFTPGTYEWVPGEAS
                     AFNPPLYSRTAAAANSDTASWTNPNTWTANSDGSGAAAGTVPDNTVPVTILSGHGVKT
                     VTNSDASTSLTLNGRLTIDSTTGNNFGEIDGAGTLILKRGSFPTYTVLGSDFFTVGTG
                     GTVIYAGVNASYTLPASPTDYNNLVIDSGGTKTLAGYTTVNSLTVGAASTLGQATTLA
                     TNNNTIEIKDDYELNGSFTHGTGTVYFNGSTGSQSLGANSVAATLNFHHLIFDNNTAK
                     TLGKPITTTGNLTITSSASIITQSSNDITVAGNWLNNGLSTVLSTTTGDVIFNSTTAA
                     QSLTGSTNFADLTISKTGQVITLNDSIRVTGDLSITGGTLALANESMSINGNISNAGS
                     ITHSSGTVHFTGNSTQTLNGAGSASLNNVTVNGSSNTVAANANYDVTGNLRVVAGTFA
                     PSATDAYYNVDSLVVHSGGTFDGTNLTATWLSGSFRNHGTLTALADTTVFKATAAGLT
                     VTGGPTFENLRIIDSGRVTLSSNTSVSDELQLVGGFIVTGSNSLILTKTATDPVTGGD
                     SLSFVDGNVVITWPASQTYVARTFPTGNALYRFFRPIEIAASTDATPPTVRASLTRSP
                     ANAVTLNGGLVKVSDIRYHTVTRTAGNDFTGSDLTARLVYARRVLSDYVTAEANLRVA
                     RSTDFSTWDNIGGSGVDTLESATTDGDTAGYILSNATSLAGSNSPTYFTLGTTTFDNS
                     LPVELAYFRAMPTDGQIRVEWRTESETENAYWLVQRREVNDEEAEHYRSTGKLSNTNA
                     QYQTVSSIEGQGTKASATDYNYLDKSAETGKMYAYRISDVGYDGTVTAHNVVVVSMDM
                     PRTFALKSNYPNPFNPTTTIRYELPVSAKVTIKVYNILGQEVTTLVDADQTSGFQKVV
                     WNGLNKYNQQVASGVYIYRMIAVSKDGKQSFTQTKKMMMLK"
BASE COUNT         1379 a         1055 c          980 g         1412 t
ORIGIN      
        1 cgtttttttt tagcgatatt ccgaccggaa ccacactgca tgaacataac ggcaatggta
       61 ctcctatgag cgccgctttc cataccgatc tccaggaaga atccccaaga tgcttaccga
      121 gcatttcaac tgtaaagaga cctcttaata taccggctag gataaaacca ataagtaaat
      181 aaaatgacgc atcaacaagt gtatcggcta ttgatataaa aatagattgt aacatatgtg
      241 tgtaatatat gctctatctg acgttaaacc aagtccatca ttgatatttc actgtatcaa
      301 ttacatcata aactgtactg ttattactta aagaattttc ttgcatttcc atgtctcgcc
      361 gtatatattt gcgacgtcat tggcacctgt agctcaattg gatagagcat ctgactacgg
      421 atcagaaggt ttggggttcg actccctacg ggtgcacaac aaaggcttta gttttctaaa
      481 gcctttttta ctttacggca cttcgatgca ccgcattctc gttatcatac ctgcttttaa
      541 tgcccattca acaatcaatg gcattttatc tgttttaaat caatttaaag atcgttcccg
      601 accgcttgat attgacctag acacgcttgt tgtagacgac ggttcagatc ccaggctaga
      661 aactcacctt aattcaggct acggacatat tattacacac acactcaata aaggcaaagg
      721 cgctgcttta aaatcaggtt ttaagtttgc ttccgatgaa aagtatgact atgtcgtaac
      781 gatggatgcc gacggacagc acgatcccaa tcgactgctt gagatggttt cgtttgggtt
      841 gcgcggcgac tacgatttgg taattggcga gagaaatata agcttaaaat caatgagttt
      901 ggggcgtttt ctctctaaca aaattaccac aaaaattatt cgtagaatga gcggtttaga
      961 tattaaagat agtcaatgcg gtttcagatt tatcaaaatt gacctgctgg aaaagtttga
     1021 acttcatacg gataattttg ataccgaatc tgaattttta ctacgcgctt caaaatataa
     1081 gatccaatat gctaattttc cgattccaac aatcaacaat ttatcccaca gtcatatcaa
     1141 tcacttgcgt gataccgtcc gctttatacg gcttttactg aaatctattt agttttcagt
     1201 atattaaaac aaaaaagccc atcaattgat gggctttttt tattacttct taaaagatgc
     1261 ttatttcaac atcatcatct tcttggtctg cgtgaaagat tgtttaccat ctttcgatac
     1321 ggctatcatt cggtaaatat atacaccgct tgctacttgt tggttgtatt tattcaaacc
     1381 attccaaaca actttttgga atcctgatgt ttggtcagca tcaacaagtg ttgtaacttc
     1441 ttgtccaagg atgttataca ccttaatggt aactttagca gatacaggca attcgtaacg
     1501 tattgttgtc gtcggattaa acggattcgg ataattagac ttcaaagcaa acgtacgcgg
     1561 catatccatg gatacgacaa ctacgttgtg cgctgtgaca gtaccatcat aaccgacgtc
     1621 ggaaatacga taagcataca ttttgcctgt ttcggcagac ttgtcaagat aattgtagtc
     1681 tgtcgcagag gctttggtac cttgaccctc gatacttgat acagtttgat actgtgcatt
     1741 ggtattcgaa agtttaccgg tcgaacgata atgttcggct tcctcatcat ttacttcgcg
     1801 acgctgaaca agccagtatg cgttttccgt ttcactttca gttctccatt cgacacgaat
     1861 ttgtccgtct gtcggcattg ctcggaaata ggccaactca actggcaatg aattgtcaaa
     1921 agtcgtcgtg cctaaggtaa aatacgtcgg actattcgac cctgcaagcg aagtagcgtt
     1981 ggataatata taacccgctg tatcgccatc ggttgttgcc gattctaagg tgtccacacc
     2041 ggagccaccg atattatccc acgtactaaa gtcagtagaa cgtgctactc gcagatttgc
     2101 ttcagccgtc acataatcac ttaaaacacg tcgtgcatag actaaccgcg cagtcaagtc
     2161 actgccggta aaatcattcc cagctgtacg tgtaacggta tggtatctaa tatcagaaac
     2221 tttaactaac ccaccattta aagttacagc attagccggt gagcgtgtta atgaagctcg
     2281 cactgtagga ggtgttgcgt cagtacttgc tgcaatttct atgggtcgga agaaccggta
     2341 taacgcatta cccgtaggga atgtgcgagc aacataagtt tgggaggcgg gccatgtaat
     2401 caccacatta ccgtcaacga aggacaatga atctccgcct gtcaccgggt cagttgcagt
     2461 ttttgttagt atcaatgaat tcgatcccgt tacaataaat ccaccaacta attgcagctc
     2521 atcagaaacc gatgtattcg atgacaatgt cactctaccg ctatcaatga tacgaaggtt
     2581 ttcaaacgtg ggaccgccgg taaccgtcaa acccgcagct gttgctttaa ataccgtagt
     2641 atcagccaaa gccgtcaagg taccgtgatt acggaagctt ccgctcaacc atgtagccgt
     2701 tagattggtt ccgtcaaagg taccaccact gtgtacaact aagctgtcca cgttgtagta
     2761 cgcatccgta gcggacggtg caaacgttcc ggctacaaca cgtaagttac ctgttacatc
     2821 gtagtttgcg ttcgctgcaa cagtatttga acttccatta accgtcacat tattcaatga
     2881 agcgctacct gcaccgttca gtgtctgtgt ggaattaccc gtaaaatgaa ccgttcctga
     2941 cgaatgcgtg atggatcctg cgtttgaaat attaccgttg atggacattg attcgtttgc
     3001 aagagctagc gtaccgccgg taatacttaa atcgccagtt acacgtattg agtcattgag
     3061 cgttatcact tgacctgttt tggaaattgt caaatcagca aaattggtcg atcctgttaa
     3121 actctgtgcg gcagtggtag agttaaatat tacatcacca gttgttgtac tcaaaacagt
     3181 ggataggcca ttatttaacc agtttccggc cactgtaatg tcattgcttg attgtgtaat
     3241 aattgaagct gagctggtaa ttgtcaggtt acctgtcgta gtgatcggct tacctaacgt
     3301 tttagccgta ttattatcaa atatcaagtg gtggaaattc agagtagctg caacgctatt
     3361 ggcgccaagg ctttgcgaac ctgtagaacc attaaaataa accgtaccgg tgccgtgtgt
     3421 aaaagaacca ttaagttcat aatcgtcttt tatttcaata gtattattat tcgttgcaag
     3481 ggtcgtcgct tgtcccaatg tacttgccgc accaacggtt aaggaattaa cagtagtata
     3541 acctgccaga gtttttgttc caccactatc aataactaga ttattatagt cagttggaga
     3601 agccggtaaa gtataacttg cattaacccc ggcataaata acagtaccac cggtccctac
     3661 agtaaagaaa tcagaaccta gaaccgtata agtagggaat gaaccacgtt ttaatatcaa
     3721 agtccctgct ccgtctattt caccaaaatt atttccagtc gtactatcaa tcgtcaaacg
     3781 accatttaac gttaatgagg tagacgcatc actgtttgtc acagttttta caccatggcc
     3841 tgaaagaatt gtaactggta ctgtattatc aggcacagta cctgctgctg ctccagaacc
     3901 gtcagaatta gccgtccatg tattgggatt tgtccagcta gccgtatccg aatttgcggc
     3961 agctgccgta cgagagtaca agggcggatt gaatgcggat gcctcgccag gtacccattc
     4021 ataagtgcca ggtgtgaatg tcatgctatc ttggtgacca aacacatcgc ctgctgagct
     4081 ctcaaagcca ttggaaggag ttaaccatgg gcttgatcct gcccaatagc gcggaacata
     4141 gttggtttca gtaccttgag cttctgcatt gttatagtag aacgactgta ggagtttgac
     4201 gtttgtaaat gaggttacat ctactgtcca atattgatca agtgctttag aaggcacaat
     4261 aatattcgga ttagcaccag atacagcgcg aacagcgata ttacccgtac cagtacgagt
     4321 tccaaaagtc atcaccgcag gaagataata cgaaccgtaa ccgatcggga aagtaaacga
     4381 tgctacaaca ggtgctgctg caagattttt aataatctgc ggaccgccta atgctcctgt
     4441 ttgaataata caacgggaag cactaaatga gcccgaacct gcgctgaccg tagcagaagt
     4501 atcaatagtc agtgcattac taccggttaa aataatgttc ccatcatttg acattgtcag
     4561 tgaatttttt acagttaccg gtgtttctaa tctcagattt tgagatgctg ccttagtaag
     4621 agtcaggttc cggaaagcaa tggcatttga accacctatc gtatctgtag cagttgtccc
     4681 agtaaaagta attgtattcg ccggcgtagt ccatgtacca aaatttcgca acgatccacc
     4741 aatttcaata ttattcgtac ctatagtaaa tgttccgctc ttgatgtaga gcttgttacc
     4801 tatgccccat tgtgttgaag ataaga
//
LOCUS       DAOQGF010000181         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_9310_length_4798_cov_4.955092, whole genome shotgun sequence.
ACCESSION   DAOQGF010000181 DAOQGF010000000
VERSION     DAOQGF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9310_length_4798_cov_4.955092"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2616
                     /locus_tag="PK937_15195"
     CDS             <1..2616
                     /locus_tag="PK937_15195"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNH33968.1"
                     /translation="KTAIPSLFYYNAFLIASDGLEAKSGTVSSALSRFMAWKTADGKK
                     EASHLVSQMITLIDGMLNKETLLDLIRYFTVFEKSKHEDLHTKITTIESVKKIAAYHQ
                     YYAVQKAVASTIGASSDKGTRKGGVVWHTQGSGKSLSMVFYTSKMVLALDNPTIIIIT
                     DRNDLDDQLFDTFAASTQLLRQVPVQADSRENLKSLLKVASGGIVFTTVQKFFPQDDE
                     LKFPLLSERKNIVVIADEAHRTQYGFEAKTKERKDKEGNTLGTEITYGFAKHMRDALP
                     NATFIGFTGTPVELTDRNTKTIFGDYIDVYDISQAVLDGATVRIYYESRLVKVHLEEG
                     EKEKLDDRLLMVAEGAPEYLVDNAKSKWTRIEAIVGQTDRMKEVAKDIVKHFEDRQQV
                     FIGKAMIVAMSRRIAVLLYDQIIKLRPDWHHQDDDKGAIKVIMTGSSSDPASYQPHVR
                     DKDRRKAIGERLKDDKDPLKMVIVRDMWLTGFDAPCLHTLYVDKPMRGHSLMQAIARV
                     NRVFNEKPGGLIVDYIGIASDLKKALAVYTESGGKGEPALDIDMAVRAMNEKLEVVRQ
                     MMHGYDYKTFYTAPLAEKLAIILTAEEHILSLESGKERFIREVTLLSKAYALCKSTAE
                     ADTVSAEVSFFQAVKARLAKFDTYGGGEGNEAIETVIRQLVDKAVAADGVIDIFDAAG
                     IKKPDISILSDEFMKEIQGMKFKNLAIELLRKILSDEIRTRSKYNIIQSKALSEMLEN
                     TIRKYQNNLLTAAEIISELIDLAKNVKDADKRGEKLNLSVEELAFYDALEVNDSAVQV
                     LGDEILRQIARILVERVRANTSIDWQIKENVRAKLRVIVRRVLREYGYPPDKQEKAVD
                     TVLAQAETLAEIWAA"
     gene            3148..3831
                     /locus_tag="PK937_15200"
     CDS             3148..3831
                     /locus_tag="PK937_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNH33969.1"
                     /translation="MKFQDFERIMTTARLGRYQAACGGNSKRAMTLYRKNLQLSQELF
                     TVISCFEIALRNAIDKQLLQTRGNDWLRNGATAGGIFDNPNCRLTKENIDDAMHKLNH
                     FYTHNKLVAELGFGFWRYMFAPNQFAATGKTLLQIFPAKPTSTAAIQYNHTFVFNQLA
                     QINNMRNRIAHHEPICFVPGQSVKGTTYARQHYGLILQLFQWMSIDEGKLLYGIDHIN
                     TVCNQIDAL"
     gene            4136..>4798
                     /locus_tag="PK937_15205"
     CDS             4136..>4798
                     /locus_tag="PK937_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNH33970.1"
                     /translation="MKITDYILFTINRLPRGYLFTYADFKKEVGQKEAIIKALNRMAA
                     SGKIAKLSKGKYYKPESTPFGNLQPNQAQVVKDLLEENGKVTGYLTGYSIYNRLGLTT
                     QVSNVIQIGKNQVRPRFKRELYTIAFIKQKNTITKENVPLLQLLDAIRYIKKIPDANI
                     EASCKRFLAIIKNFTDKEINTLVRLALKYPPATRALLGALLEQLRKGNTAESLFNSLN
                     PIT"
BASE COUNT         1469 a         1047 c         1126 g         1156 t
ORIGIN      
        1 aagacggcga ttccatcgct cttttattac aatgcgttcc tgatcgcgtc cgacggactg
       61 gaagcgaaat cagggacggt ctcttccgca ctgtcacgct tcatggcctg gaaaacggcc
      121 gacggcaaga aggaagcctc gcatcttgtc agccagatga tcacgctcat tgacggcatg
      181 ctgaataaag agactttgct ggatctcatc cgctatttca ccgtttttga aaagtcaaaa
      241 cacgaagacc ttcataccaa gattacgacg atagagtcgg tcaaaaagat cgccgcgtat
      301 catcagtatt acgccgttca gaaagccgtt gcgtccacca tcggcgcttc ctctgataaa
      361 ggcacgcgga aaggcggcgt cgtatggcac acacagggtt ccgggaaatc gctgtcgatg
      421 gtgttttaca cgagcaaaat ggttctcgcg ctggataatc ccacgatcat tattatcacc
      481 gaccgaaacg atctcgacga tcagcttttt gacaccttcg cggcatcgac tcagcttctg
      541 aggcaggttc cggtgcaggc cgatagccgg gagaacctaa aatcactgtt gaaagtcgcg
      601 tcgggaggca ttgtatttac caccgtgcag aagtttttcc cgcaggacga cgaattgaaa
      661 tttccgttat tatccgagag aaaaaacatc gttgtcatag ccgacgaagc gcaccgcact
      721 cagtacgggt ttgaagccaa gaccaaagaa agaaaagaca aagaaggtaa tacgctcgga
      781 acggagatca cgtacggttt tgccaaacac atgcgcgacg ctttgcccaa tgcgacgttt
      841 atcggattta ccggtacgcc ggtggaactc accgaccgga atacgaaaac cattttcggt
      901 gattatatcg acgtgtacga tatttcccaa gccgttctcg acggcgcgac ggttcgcata
      961 tactatgaaa gccggttggt aaaagttcat ctcgaagaag gcgagaaaga aaaactcgac
     1021 gaccgacttt tgatggtggc cgaaggcgcg ccggaatatc tcgttgacaa cgcaaaatcc
     1081 aaatggacgc gtatcgaagc tattgttggc cagaccgacc gtatgaaaga agtcgccaaa
     1141 gatattgtaa aacattttga agatcgtcag caggtattta tcggcaaggc aatgatcgtg
     1201 gcgatgagcc gccgtattgc ggtgcttctg tatgaccaaa ttatcaaatt gcgccccgac
     1261 tggcatcatc aggacgacga caaaggcgcg atcaaagtga tcatgaccgg ttcctcatcc
     1321 gatccggcga gttaccagcc gcatgttcgc gataaggata gacgcaaagc catcggcgag
     1381 cgattgaaag atgataagga cccgttgaag atggtgatcg ttcgcgatat gtggctcaca
     1441 ggatttgatg cgccgtgcct gcacacgctg tatgtggaca aacccatgcg cgggcacagt
     1501 ctcatgcagg cgattgctcg ggtgaaccgc gtattcaatg aaaaacccgg cggattgatc
     1561 gtggactata tcggcattgc ttcggatttg aaaaaagcgc tagcagtcta taccgaaagc
     1621 ggcggcaagg gcgaaccggc gctggatatt gacatggccg ttcgtgcgat gaacgaaaaa
     1681 ttagaagttg tccggcagat gatgcacgga tatgattata aaacgtttta taccgcgccc
     1741 ttggctgaaa aattggcgat cattctgacc gctgaagagc atatactatc tctcgagagc
     1801 ggtaaggagc gttttattcg tgaagtaacg ctgctgtcca aagcgtatgc gctatgcaaa
     1861 tctacggcgg aagccgatac ggtgtcggcg gaagtttcat tcttccaggc ggtgaaagcg
     1921 cggctggcga agtttgacac ctacggcggc ggagagggca atgaagctat tgagaccgtg
     1981 attcgccaat tagttgataa ggcggtcgca gcagacggag taatcgacat atttgatgcg
     2041 gccggaatca agaagccgga tatttcgatt ctgtccgatg aattcatgaa agaaattcag
     2101 ggtatgaaat ttaagaacct tgcgatcgaa ttgctccgca agattctgag cgatgaaatt
     2161 cgtacacgat cgaagtacaa cattatccag agcaaggcgt tatcggagat gctagaaaat
     2221 acgattcgca aatatcagaa taatctgctc accgccgctg aaattatcag cgaattaatt
     2281 gatttagcga agaatgtcaa agatgcagac aagcgaggcg agaaactcaa tttgtccgtt
     2341 gaagaactgg cgttttacga tgccctggaa gttaacgaca gcgcggtgca ggttctaggt
     2401 gacgagatct tgcgccagat tgcgcgcata cttgttgaac gagttcgcgc caatacgtcg
     2461 atagactggc agatcaaaga aaatgttcgc gcaaaattgc gcgtgatagt tcgccgcgtc
     2521 ttacgcgaat acggttatcc gccggacaaa caggaaaagg ccgttgatac ggttcttgcg
     2581 caggccgaga ctttggcaga gatatgggcg gcgtgaatgt tgggatcata aggaatatca
     2641 ataactacgt tcccttctcc ccttacaaca aaaatttgcc gcgctggttg agaaagttga
     2701 acccatgcac gccaagcagc gcgcgtcgga gcaggaattg gatttgcttt ttcaagcatt
     2761 aatgcaaaga gcatttaaag gagaattggt aagttgactt gccctgagcg atgcgccttc
     2821 ggcgcagagt aaagaggcat agattgttta aggaagacaa gcacagcgtg caagattggg
     2881 gctttgtgat tcacgttcgg agattcagca cttattgatt atgtcagttt acaggatgct
     2941 ctgtaatcct tgagaacaca aacccacaaa ccattggtgg caatatcgga gaccactatg
     3001 aattttgagc agattactga aaattataga cttagaaacc ctcataaaaa acgaaacccg
     3061 atagtttgaa ttgcttcagg gactaccggg aataagacat cattaacata catacatttg
     3121 aacgataaaa gcaaataaaa gtttacaatg aaatttcagg actttgaaag aataatgaca
     3181 accgccagat tgggtcgcta tcaagcggct tgcggtggta atagcaaaag agccatgacg
     3241 ctttaccgaa agaacctgca actttcacag gagcttttta ctgtcatcag ttgctttgag
     3301 attgctttgc gaaacgccat agacaagcag cttcttcaaa cacgcggaaa tgattggttg
     3361 cgaaacggcg caaccgcagg cggaattttt gacaatccca attgccgatt aacaaaagaa
     3421 aacattgacg atgcaatgca taagttgaat catttttaca cacacaacaa acttgtggct
     3481 gaattgggtt ttggattttg gcgttacatg tttgctccaa accaatttgc tgcgaccggc
     3541 aaaacgcttc ttcaaatatt cccggctaag ccaacaagca cagcagctat tcagtataat
     3601 catacatttg tatttaatca gttagctcaa ataaacaata tgcgaaaccg catcgctcat
     3661 cacgaaccta tttgttttgt tccaggacaa tctgtaaaag gaacaactta tgcacggcaa
     3721 cactacggac tgattttgca attatttcaa tggatgagta tcgacgaggg aaaattactt
     3781 tacggaattg accacattaa cacagtttgt aatcaaatag atgccttata aaacaagatg
     3841 caacttgtag agaaagaaac cctgccacca aaacgggttt ggggcgaaat gctaaaatca
     3901 agtgcagttt ttcaatttaa atttagcgct tattgatccc gcaagtttgt gggatgcgcc
     3961 gaaatctccc actgcgccaa gccgtgtccg ttaaccaagg tgttaaagaa aaatagagta
     4021 tggtaacttc acaagaccgt tgcgaggtta caatcatttg gtttaatctt aaaatgtcca
     4081 gtttttttaa taataaactg gacaaagcaa ttgcttttta ttataatcat acataatgaa
     4141 aataactgat tatatattat ttacaattaa tagactccca agaggatacc tattcaccta
     4201 tgccgatttt aaaaaagagg tgggtcaaaa agaagcaatc ataaaagccc tgaaccgtat
     4261 ggcggcttcg ggcaaaattg ccaaactttc caaaggaaaa tattacaagc ccgaaagcac
     4321 cccatttggc aatcttcagc ccaatcaggc acaagtagta aaagatttgt tggaagaaaa
     4381 tggaaaagta accgggtatc tcaccggcta tagcatttat aaccgattgg gcctaaccac
     4441 acaggtaagc aacgtcatac agataggtaa aaatcaagtt cgcccccgtt ttaaaagaga
     4501 actctatact attgctttta taaagcaaaa aaacaccatc accaaagaaa atgtcccatt
     4561 gttgcagtta ctggatgcta tccggtacat caaaaaaata ccggatgcca atatagaagc
     4621 atcctgtaaa cgatttttgg caatcatcaa aaactttacg gacaaagaaa tcaataccct
     4681 ggttcggtta gcattaaaat atccacccgc tacaagggct ctattaggtg cattattgga
     4741 gcaattgcga aaaggcaaca cggctgaatc acttttcaat tcattgaacc cgattact
//
LOCUS       DAOQGF010000182         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_9701_length_4665_cov_7.873319, whole genome shotgun sequence.
ACCESSION   DAOQGF010000182 DAOQGF010000000
VERSION     DAOQGF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9701_length_4665_cov_7.873319"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>4665
                     /locus_tag="PK937_15210"
     CDS             42..>4665
                     /locus_tag="PK937_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNH33971.1"
                     /translation="MAVQTGDPYWEAQGGTIPPGLSGTVNAIAVTGSDVYVGGSFSSM
                     NGNTTAAYIAKWNGTSWEALGTGVNNTVNAITISGSDVYVGGSFTTAGGVSTPGIARW
                     DGSAWNSVGGGITSSNVRAIAVSGSNIYAGGAFTDANGNTSADNIALWDGASWNALGT
                     GLSATVNTIVIGNTKVYVGGNFQNAGGDANADRIAYWDLDSVKWKSMSTGSASQVFSL
                     AFQSDTSLYVGIANAPGIRKWNGTVFSTIGSTSLSSAVRTIVLNGSEIIVGGDFTLAS
                     PRLVRIAKWTGSAWQSVDSLSNTVNAIASSSDGKLYIGGAFANEHGMSNANSIVQWNG
                     TRLIPIGTGLANTVRAIVASGTTVSVGGDFTDADTLTKADYAARWNGFTWSAMQSGFK
                     GSVYSMSQSGTTIFAGGSFTQIGTGTKVNNVARWTGSTWGTMNVSGGTANTVGTNGSV
                     NAVAAVTTTRAYVGGSFTKLGDSTVVNGIARWTNGVGWEALGSGVSGGNVHALALSGT
                     KLYVGGDFTSASGVANTSHIAVWDTATGLWSALGSGISNNTVYSIVVNGSDVYVGGNF
                     TDAGGVAAADYIAKWDGVSWSSVGTGTEINNTIRTMAFLGTDLYVGGDFTDAAGYSGG
                     DYIAKWSGSAWSALGDGANNSIYAIIAAGSDLYAGGNFTSIDGSSASRFARYRVPDVT
                     APSTPTNLTASTGPNQLVLKWKKNSDVDFSKYRIYFGSTSNPTVQTDSSLSISDTSRT
                     MTGLTNGTTYYFRVSAVDIWGNQSSLSTQASGIPNDTVPPSTPSLISATGGNLAVNLT
                     WSKNNDSDFKRYRIYRGTSPGATTQVDSVSIVGDTTKSISAPIPGVTFYFRVRTLDSA
                     NNLSTYSTELSASSLDQTPPSTITGLSTTASDTTVILRWRQSTATDFSKYYIYMDTLA
                     LPVTVVDSALTKADTARTFTHLTNGKTYYFRVTAADTFANVSAFSSVASATPFDQTPP
                     SAPQNLAAVASDASVVLHWDANTENDLLRYRVFVGTSSGNEVQTDSTVYDSIVVNGLN
                     NDTEYFFRIAAIDTGKNQSNYSNEVSATPFAANAAPTNPVLLAATAQNQSVVLTWQKN
                     PDTDILKYYVVAGLSSNPTALVDSTNGDTTVTLSGLTNEQAYYFRIIAVDSALQVSGY
                     SNELSQSPYDNVAPATPLNFIASAGDQSAQLTWNNDNTEPIKIYRVFINGTQTDSLTD
                     TTYTVTGLTNYTSYQVSVAAVDSNDNVSSNTSDIGVTPIDLTAPSIPNNLTAEAGDAS
                     VHLVWSVNTESDLARYRIFINDVQSDSTSDTSYTATALTNYTLYSFKIAAVDTSGNFS
                     GFVSSTSMPYDQTAPAVPQDLAATAGDGQVTLNWTANGEGDFAHYRVYMDGTQIDSTT
                     NNSYTKTGLSNYTTYNFKLSAVDTSRNTSALTSQVSVMPYDQTAPAVPQDLAATAGDG
                     QVTLNWTANGEGDFAHYRVYMDGTQIDSTTNNSYTKTGLSNYTTYNFKLSAVDTSRNA
                     SALTSQVSSMPYDQTAPSGVTGLSVSRGDGALTLNWTANGESDFKAY"
BASE COUNT         1243 a          980 c         1154 g         1288 t
ORIGIN      
        1 tagcctcttg gtcgaagtca aatctcaatt cgatcaaacc catggctgtc caaacgggag
       61 atccctattg ggaagcgcaa ggcggaacaa taccaccggg gttatccggt accgtaaacg
      121 ctatcgctgt taccggtagt gatgtgtatg tcggtggatc ttttagctca atgaacggta
      181 ataccacggc tgcttatata gccaagtgga atggcacttc gtgggaagcc ttaggcaccg
      241 gtgtgaataa tacggtaaac gccattacca tatcgggttc tgatgtgtac gtcggaggtt
      301 cgtttacaac ggcgggaggt gtatccacac ccggtattgc acgttgggat ggaagtgcat
      361 ggaactctgt cggcggaggt attacctcct caaatgttcg agctatcgcc gtatccggat
      421 ctaatattta tgccggcggt gcatttacag atgcaaatgg gaataccagc gctgacaata
      481 ttgctttgtg ggatggagct tcatggaatg cattaggaac tggtcttagt gcaacagtaa
      541 ataccatagt tatcggaaat acaaaagttt atgtaggagg aaactttcaa aatgctggtg
      601 gtgatgctaa cgcagatcga atagcatact gggatttgga cagtgttaaa tggaaatcta
      661 tgtcaaccgg atcagccagt caggtgtttt ctttagcgtt tcaatcggat acaagtttat
      721 atgttggtat agctaacgct cctggtataa gaaaatggaa tggaacagtt tttagcacga
      781 tcggctcaac aagcttgagt agtgcggttc gaactatagt gcttaacgga tcagaaataa
      841 tcgtcggagg ggattttact cttgcttcac ctcgtttggt aagaatagcg aaatggacag
      901 gaagcgcatg gcaatccgta gatagtttgt ctaataccgt taatgctatc gcttcttcat
      961 cggatggtaa gttatacatc ggcggtgctt ttgcaaatga acacgggatg tcaaatgcta
     1021 attccattgt tcaatggaat ggaacacggt taatacccat aggaactgga ttagctaata
     1081 cggttcgcgc tattgtagca tcgggtacga cggtgtctgt gggtggtgat tttacagatg
     1141 cagacacatt gaccaaagca gactatgctg cgcgttggaa tgggtttacc tggtcggcca
     1201 tgcaaagtgg tttcaaaggc tctgtatact cgatgtcgca atcgggaact acgatatttg
     1261 cagggggttc tttcacgcag ataggtaccg gaacaaaagt aaataacgtg gcacgatgga
     1321 ccgggagtac gtggggtacg atgaatgttt caggtggtac tgctaataca gttggaacga
     1381 atggctcggt caatgccgtc gcggctgtaa caacgacgcg tgcgtatgtg ggaggtagtt
     1441 ttacgaaact gggggattct acggttgtta atggtattgc gcgttggacc aatggcgtag
     1501 gttgggaagc tttgggatcg ggcgtttccg gtggcaatgt tcatgctttg gctctttcag
     1561 gtacaaagct ttatgtcggc ggtgatttta catcggccag cggtgttgcc aatacatctc
     1621 atatagctgt ttgggataca gcgaccggtt tatggtcagc tttaggttca ggcatttcta
     1681 ataacacagt gtattctatt gttgtcaacg gttcagatgt gtatgtcggc ggtaatttta
     1741 cagatgccgg cggcgttgcg gcagcggact atattgctaa atgggatggc gtttcatggt
     1801 ccagtgtcgg tacaggtaca gaaataaata ataccattcg cacgatggct tttttgggaa
     1861 cagatctgta tgtcggcggt gattttacgg atgcggccgg atattcgggt ggtgattata
     1921 ttgcgaagtg gagcggatca gcctggtctg cgcttggaga tggtgccaat aattcgattt
     1981 acgcgattat tgctgccggt tcggatttat atgccggagg aaactttaca tccattgacg
     2041 ggtcttcagc atcgcggttt gcacgatacc gcgtgccgga tgtgacagcg ccaagcacgc
     2101 cgacaaacct gactgcatct acaggtccga accaacttgt tttaaagtgg aaaaaaaatt
     2161 cggatgtaga tttctctaaa tacagaatct attttggaag cacctcgaac ccaactgtac
     2221 aaacggattc ttcgctcagt atatccgata cctcaagaac tatgaccggt ttgacaaacg
     2281 gtacgacgta ctatttcagg gttagtgctg ttgacatttg gggtaatcaa agtagtttgt
     2341 caacacaagc gagcggaata ccgaacgata cagtgccgcc atctacgcct tcgcttattt
     2401 cagcaaccgg cggtaatttg gcagttaacc ttacctggtc aaaaaataac gattcagatt
     2461 tcaaacgtta ccgcatttac cggggtactt cgcccggagc cacgacccag gtggattctg
     2521 tatccatcgt cggtgatact acgaaaagca tttcagcgcc gatacccgga gttacatttt
     2581 acttcagagt gcgtacttta gattcagcaa ataacctgag cacatattcc accgaattat
     2641 cagcatcttc cttagatcaa acgccaccat ccacgattac ggggctatct acgactgcga
     2701 gtgatacgac ggttattctg cgctggcgtc aaagtacagc gacggatttt agtaaatact
     2761 atatctatat ggatacgctg gcgctgcctg taacggtggt ggattcggcg ctgacaaaag
     2821 cagataccgc acgaacgttt acgcatttga ccaatggtaa aacgtattat tttcgcgtga
     2881 cggcggccga tacatttgct aacgtatcgg cattttcatc ggttgcgtca gccactcctt
     2941 ttgatcagac accgccttcc gcgccgcaaa atcttgccgc ggtggcaagt gatgcttcag
     3001 ttgtattgca ttgggatgcc aatacggaaa acgatctttt gaggtatcgt gtatttgtgg
     3061 gcacttcctc tggtaatgaa gtacaaaccg attctacagt gtatgatagt attgtagtga
     3121 atggcctgaa taacgatacg gaatacttct tccggatcgc cgcgatagat acaggcaaaa
     3181 atcaaagcaa ttattccaat gaagtgtcag ctacgccatt cgcagccaat gccgcaccga
     3241 cgaatccggt tttgttggca gctacagctc aaaatcaatc ggtagtattg acgtggcaaa
     3301 aaaacccgga taccgacata ttgaaatatt acgttgttgc aggattgtct tctaatccga
     3361 cggcattggt ggactctacc aatggtgata cgacagtgac attgtccgga ttgaccaatg
     3421 aacaagcgta ttatttcaga atcatagctg ttgattcagc tttgcaggtc agcggatatt
     3481 ccaatgaatt atcccaatct ccttatgata atgttgctcc tgctacaccg ttaaatttta
     3541 tcgcatcggc aggtgatcaa tcggcgcaac tcacatggaa taatgataac acggaaccta
     3601 ttaaaattta tcgtgttttt attaatggta ctcaaactga ttcacttacg gacaccacgt
     3661 atacagtgac gggtttgaca aattatacgt cgtatcaagt tagtgttgcc gctgtagata
     3721 gtaatgataa tgtttcatct aatacttcag acatcggcgt aaccccgatt gatttgactg
     3781 cgccttccat tcccaacaat cttacggctg aggccggcga tgcctcggtg catctagtat
     3841 ggtctgttaa tacggaaagt gatctcgctc gctatcgtat ttttatcaat gacgtacaaa
     3901 gcgattcgac gagcgatacg tcttatacag caactgcttt aactaattat acattgtatt
     3961 ctttcaaaat agcagccgta gatacgtcag gtaatttttc gggttttgtg tcttcgacgt
     4021 caatgcctta cgatcagaca gcgcctgcag ttcctcagga cttggctgca acggcgggcg
     4081 atggtcaggt tacattgaac tggacagcaa acggtgaagg tgattttgca cattatcgtg
     4141 tttatatgga tggtacacag attgattcga cgacgaacaa tagctataca aagaccggct
     4201 tatcgaatta cacgacatat aacttcaagt tatctgccgt tgataccagc cgcaatacaa
     4261 gcgcattgac ttcacaagta tcggttatgc cttacgatca gacagcgcct gcagttcctc
     4321 aggacttggc tgcaacggcg ggcgatggtc aggttacatt gaactggaca gcaaacggtg
     4381 aaggtgattt tgcacattat cgtgtttata tggatggtac acagattgat tcgacgacga
     4441 acaatagtta tacaaagacc ggtttatcga actacacgac atataacttc aagttatccg
     4501 ccgttgatac cagccgcaat gcaagcgcat tgacttcaca agtatcatct atgccttacg
     4561 atcagacagc tccttcagga gtaaccggtc ttagtgtaag tcgtggagat ggtgcattga
     4621 cactaaactg gacagcaaat ggagaatccg attttaaagc ctatc
//
LOCUS       DAOQGF010000183         4631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_9801_length_4631_cov_4.739510, whole genome shotgun sequence.
ACCESSION   DAOQGF010000183 DAOQGF010000000
VERSION     DAOQGF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9801_length_4631_cov_4.739510"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PK937_15215"
     CDS             complement(<1..1229)
                     /locus_tag="PK937_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNH33972.1"
                     /translation="MILRNILSTILLIIIFSLAGCQANHTTAQSDAGKSSVILISLDG
                     FRYDYLEKYTTPNLTALAKRGVRSEALISVFPSKTFANHYSIITGLYPAHHGIISNQF
                     WDPEYQKAFSYKDSNANKDSHWWGGEPLWNTVEKQNIKAASFFWPGSEALIQGHRASL
                     WKRYNGAVRNYDRIDTVLSWLTLPQNLKPRFIATYFSITDEVGHEFGPESQEMRRAVM
                     HVDSVIGSLVEGIDRMGLSEQVNIILVSDHGMATVLPDSLIFLDDMIDVNSVRILNET
                     PFVGIWTKSGNVDEVYKTLVNRHPRMQVYTRENVPARFKFKGNPRIPEIICLTEPPWS
                     ITTRDYARRYKDRWLYGSHGFDPEIKEMGGLFIASGPAFEKGKMIPSFENIHIYTLVC
                     HILGISPSANDGDIKVF"
     gene            1356..2963
                     /locus_tag="PK937_15220"
     CDS             1356..2963
                     /locus_tag="PK937_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNH33973.1"
                     /translation="MSIINEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAVQLAGSVT
                     ARKNQRTSRSDWMELEKQRGISISSTVLQFEYTGFKINLLDTPGHQDFSEDTYRVLTA
                     VDAVVMVIDAAKGIEAQTRKLFEVCRRRRVPIFTFINKLDRPTLEPLALIDEIESILQ
                     IHVYPMNWPLGTGVDFRGVLDRQAKEVHLFERTTGGAYRAPVSVFDVSDPLVKNMMSP
                     NTYATVVEELEMLDAAGASFDVKAVHRGELTPVFFGSAMNNFGVQLLLDGFLRYSIEP
                     APRIVDERIIEPQMEAFSAFIFKIQANMDPKHRDRIAFMRVCSGKFSRDMMVTHTRTG
                     KKVRLSNSHKLFGQQRETVDDAYAGDIVGLVGHSEFGIGDTLTEDQTIVYKEIPRFPP
                     ECFSFLHNPNPSKFKRFREGLEQLLQEGVVQAFYLKNSYEKVPILAAVGPLQFEVVQF
                     RLESEYGAECRLESTNWKVVRWVSPEADWEKISKIYLPTGAAYAEDMAHETVILFPDE
                     WTCNYFIEKNPEVQLSSFSYKDATVLD"
     gene            complement(2960..3607)
                     /locus_tag="PK937_15225"
     CDS             complement(2960..3607)
                     /locus_tag="PK937_15225"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HNH33974.1"
                     /translation="MIHRDYIMRLVDLCAKALARVMNMRQPQEQQQALEDIRSTLAQA
                     VSVSPDLLDSMTEMDLITLLRKSEWTAELFDSVGELLLQQGRILKSENKEMEAARVFR
                     KAWLCFYEASLNVGDFQSRSEKMDFCITHDILYAGNPGYYQTVLEWLTRAGRYEKGED
                     WLFDMAEAGIAADSDAEKFFGWLKLMPDDVLKKSGMPRDEVISSQETYDRKFKRV"
     gene            complement(3675..4325)
                     /locus_tag="PK937_15230"
     CDS             complement(3675..4325)
                     /locus_tag="PK937_15230"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNH33975.1"
                     /translation="MRLLLLGAPGVGKGTQSKLLVEEFKIPQISTGDMLREAIRNNTP
                     LGLKAKSYMDQGQLVPDEIIIGLVEERLRLDDAHYGFILDGYPRTIPQAEALDALLSR
                     MSIQIDAVIDINVDSEELVNRLSHRIICRSCGAVYNKVTNPPKVEGVCDKDGGEIYQR
                     SDDKKEAIENRLKVYEKETAPLREYYRKRGKLHAIDGQLPMGVVYKKILAILEVEK"
     gene            complement(4340..>4631)
                     /locus_tag="PK937_15235"
     CDS             complement(4340..>4631)
                     /locus_tag="PK937_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33976.1"
                     /translation="TIATDDADYRDWVFEAIRDSGCWESLIPEGFSSQLEGYIQTKYE
                     RKWKAMGKPCYYMRYRKTTNPVFSTDHYITEQNLQFPLRKYAEELQSDIVEL"
BASE COUNT         1300 a         1068 c          964 g         1299 t
ORIGIN      
        1 ttaaatactt tgatatcgcc gtcattggcc gaaggcgata ttccaagtat atgacacacc
       61 aatgtataga tatgaatgtt ttcaaacgat gggatcatct ttcctttttc aaacgctggg
      121 cctgacgcga taaataagcc gcccatctct ttgatttccg gatcaaaacc atgtgaacca
      181 taaagccatc ggtctttata ccgtcttgca taatcacgcg ttgtgataga ccacggcggc
      241 tccgtcagac atatgatttc aggaattctc ggatttcctt taaatttaaa acgcgccgga
      301 acgttttccc gtgtataaac ctgcatccgc gggtgtctat tgaccaaggt tttataaacc
      361 tcatccacat ttccactttt tgtccatatg cccacaaacg gagtttcatt taaaatgcgt
      421 acggagttaa catctatcat atcatcgaga aatataagac tatccggcaa aacagtagcc
      481 ataccatgat cggacaccaa aattatattt acctgttcac tcaatcccat tcgatcaatg
      541 ccttcgacca acgaaccgat gacggaatca acatgcatta cggctcggcg catctcttga
      601 gattccggac caaattcatg tccgacttca tcggtaatac taaaataggt agcaataaat
      661 cgaggtttca agttttgagg caacgtaagc caggaaagca cagtatcaat tcggtcatag
      721 tttcgcacag caccgttata tcgcttccac aaactggcac gatgaccttg aattagcgct
      781 tcggatcccg gccaaaaaaa agatgcggct ttgatgtttt gtttttctac cgtattccac
      841 aaaggttctc caccccacca atgcgaatct ttattggcgt tggaatcttt ataggaaaag
      901 gccttttgat attccggatc ccagaattga ttcgaaatta tcccatgatg cgccggatat
      961 aaacccgtaa ttatcgaata atgattagcg aaagttttag acggaaaaac ggatattaac
     1021 gcctcggaac ggacgccgcg tttggccaaa gccgtcaaat ttggagtcgt atatttttcc
     1081 aaataatcat aacgaaatcc atccaatgaa atcaaaataa cggatgattt gcctgcgtcg
     1141 gattgcgcgg tagtatgatt agcttggcat cccgccaagc taaagatgat gattaaaaga
     1201 atagttgata aaatgttgcg tagtatcaca aaagcactcc caataaggat tttacagaga
     1261 tgcgactaac atactcaatg cacaaataaa agaaaatatt taattgcagg ttgttgcttt
     1321 tttgtctaat atgcgcgccg aattgaagga aaaccatgag cataatcaac gaaataaaaa
     1381 aaagacgcac gttcgcaatt atttctcacc cggacgccgg taagacaacc ctgacggaga
     1441 aatttttgtt atatggcggc gctgtacaac tggccggatc ggttacggcg cgtaaaaatc
     1501 aacgcacgtc tcgttctgat tggatggaac tcgaaaagca acgcggtatt tcgatatcct
     1561 ctactgtttt acaatttgaa tatacaggtt tcaagatcaa cttacttgat acgcccggcc
     1621 atcaagattt ttccgaggat acttatcgtg tattaacagc cgtggatgcc gtggttatgg
     1681 tgattgacgc agccaaaggt attgaagcac aaacgcgtaa actttttgaa gtgtgccggc
     1741 ggcggcgcgt accgattttc acgtttatca ataaattgga tcgccctaca cttgagcctt
     1801 tagcactgat cgatgaaatc gaaagtattt tgcaaatcca cgtgtatccg atgaattggc
     1861 cattgggaac cggcgtggat tttcgcggcg ttttagatcg tcaagccaaa gaagtacatc
     1921 tttttgaacg cacaaccggc ggcgcgtatc gtgcaccggt ttccgtattt gatgtcagcg
     1981 acccgcttgt caaaaacatg atgagtccca atacttacgc gactgtagtc gaagagttgg
     2041 agatgctgga tgcggccggt gcttcctttg atgtaaaagc ggttcatcga ggtgaactca
     2101 cccccgtttt ttttggcagt gcaatgaata attttggtgt gcaactgcta ttggacggat
     2161 ttcttcgcta ctccatcgaa cccgcaccgc gcatcgtgga cgaacgtatc atcgaaccac
     2221 aaatggaagc cttttccgct tttattttta aaatacaagc caatatggat cctaaacatc
     2281 gcgatcgtat tgcctttatg cgcgtttgtt cgggtaagtt ttctcgcgat atgatggtta
     2341 cgcatacacg aaccggaaaa aaagtacgcc tgtccaattc gcataaatta tttggtcaac
     2401 agcgggaaac cgtagatgac gcgtatgccg gtgatattgt agggttggtc ggtcactccg
     2461 aatttggcat cggagatacg ctaacagaag atcaaacgat cgtgtacaag gaaatacccc
     2521 gatttccccc ggaatgcttt tcgttcttac acaatcccaa cccatcaaaa tttaaacgtt
     2581 ttcgagaagg gctggaacag ctattacagg aaggcgtggt acaagctttt tatttaaaaa
     2641 attcttacga aaaagtaccg attttggccg ccgtcggacc tttacaattt gaagtcgtac
     2701 aatttcgcct ggaaagtgaa tacggagcag aatgtcgact ggaaagtaca aattggaaag
     2761 tggtaagatg ggtttctccc gaagccgatt gggaaaaaat ttcaaaaatc taccttccga
     2821 ccggtgcagc atacgccgaa gatatggccc acgaaacggt tattctcttt ccggatgagt
     2881 ggacgtgtaa ttattttatc gaaaaaaatc cggaagtgca gttatcatcc ttttcttata
     2941 aagatgccac cgtactggat taaacgcgtt taaattttcg gtcatacgtt tcttgcgatg
     3001 aaatcacctc atcgcgcggc atacccgatt ttttgagtac gtcatccggc ataagtttca
     3061 gccacccaaa aaatttttcg gcatccgaat ctgcggcgat gccggcttcc gccatatcaa
     3121 ataaccaatc ctcgcctttt tcataacgtc cggctcgagt taaccattcc aatacagtct
     3181 gataataccc cggatttcct gcgtacagta tatcatgggt tatacaaaaa tccatttttt
     3241 cagatcgtga ttggaaatca cctacgttga gactggcctc ataaaaacac agccaagcct
     3301 tacgaaaaac acgcgccgct tccatttctt tgttttcact tttcagaatg cgcccttgtt
     3361 gtaataacag ttcaccgacg ctatcaaaaa gttcggctgt ccactctgat ttcctcagca
     3421 aagtgatcaa atccatttcc gtcatactat ccagaagatc cggtgaaacg gaaacagcct
     3481 gtgctaaagt agaacgaatg tcttccagcg cttgttgttg ttcctgcggt tgccgcatat
     3541 tcataacgcg tgccaaagct ttggcgcaga gatcaactaa gcgcatgata taatcgcgat
     3601 gtatcatagt ttgtctaaca ctttaaacat aaaaaaagcc gcgtgatgcg gctttattac
     3661 tttgggatca aatttcattt ttcaacttct aaaatggcga gtattttttt atacacgaca
     3721 cccatcggta attgtccgtc tatagcatgg agtttgccgc gtttacggta atattcacga
     3781 agcggagccg tctctttttc ataaaccttg agccggttct ctatggcttc ctttttatca
     3841 tcggatcgct gatatatctc tccaccgtct ttatcgcata caccttcgac tttgggcgga
     3901 ttggtcactt tattgtaaac ggctccgcag ctacggcata taatacggtg cgaaaggcgg
     3961 ttcacgagtt cttcggaatc cacattgatg tcaatcaccg cgtcaatctg gatgctcatg
     4021 cgcgataaca aggcgtctaa cgcctctgcc tgcgggatag tccttggata tccgtcaaga
     4081 atgaaaccgt aatgcgcatc atcaaggcgc aagcgttcct cgacaaggcc gatgatgatt
     4141 tcatcaggta ctaactggcc ttgatccatg taggatttgg ctttaagacc gagcggcgta
     4201 ttattccgaa tggcttcgcg caacatatca ccggtggaaa tttgcggaat tttaaattcc
     4261 tccaccaata atttggattg cgtgccttta ccgacaccgg gggcgcccag caacaataaa
     4321 cgcatgcgaa tgattcctcc tataattcaa caatatcaga ttgtagttct tcggcatatt
     4381 tccgcagtgg aaactgcaaa ttctgttcgg taatataatg atccgtcgaa aaaaccgggt
     4441 tggttgtttt ccggtaacgc atataataac agggtttacc catcgccttc catttgcgtt
     4501 cgtattttgt ttgaatgtag ccttctaact gtgacgaaaa accttcgggt atcagagatt
     4561 cccaacaacc ggaatcgcga atcgcttcaa acacccaatc tctatagtct gcatcatccg
     4621 tcgctatcgt c
//
LOCUS       DAOQGF010000184         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_10065_length_4560_cov_6.069034, whole genome shotgun sequence.
ACCESSION   DAOQGF010000184 DAOQGF010000000
VERSION     DAOQGF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10065_length_4560_cov_6.069034"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK937_15240"
     CDS             <1..792
                     /locus_tag="PK937_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNH33977.1"
                     /translation="ENYLIPFVQKKPSSHVVRYKTIRTTGIPESNLFETLRSQVQSYR
                     ERLDIAFLPKLTVGVDLRFTVQNMTAEKADSLLNEAEQAFRQAIESTYPHGVYGEGTI
                     SLEDVVADLLFRHNLTIATAESCTGGLIAHRLTNVPGSSRYFMQGITTYSNAAKVQHV
                     GVTQTLLDEHGAVSEPVVRAMAEGMRRVAQTDIAIATTGIAGPTGATEAKPLGLAYVA
                     LATASGTHVFKHSPLPFSIERLAFKERLSQFALDRLRHYLIGLSE"
     gene            789..1058
                     /locus_tag="PK937_15245"
     CDS             789..1058
                     /locus_tag="PK937_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNH33978.1"
                     /translation="MIICKVTGSVVSTHKNEYLHGHKIMVVQPVDLEKNHIGSDMLAL
                     DEVSAGVGDLVLVMREGGSARIVFGQKKLPVQAVIVGVIDDYDYR"
     gene            1159..2448
                     /gene="murA"
                     /locus_tag="PK937_15250"
     CDS             1159..2448
                     /gene="murA"
                     /locus_tag="PK937_15250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNH33979.1"
                     /translation="MEKFIVEGGHRLSGTFQPAGNKNEALPVIAACMLTDEPIILKNM
                     PDVSDARHMLDIAANIGAEVTRLGPSEWKVQAKSIRTHEPDYALASEIRGSFLFAGSL
                     LGRHGRVSLPSPGGDKIGRRRLDTHILALQKLGAHMDLGRKFYTMESRGRLIGEDIFL
                     DEASVMATENAVLAAVLAKGTTILNNAAGEPHVQGLCRMLNAMGAKISGIGSNILTIE
                     GVDKLHGCTHTITNDHIEIGSIIGLAAATKSEILIKNTTPRNLRMILLVFERLGVRVE
                     IRGEDLFIPGGQEPEIMSDMDGAIPKIDDAPWPGFPADLTSIMVVTATQCKGMVLIFE
                     KMFESRLFFTDKLISMGARIVLCDPHRAVINGPTPLHGELITSPDIRAGMSLLIAALC
                     AEGTTTIQNIRQLDRGYERIDERLNALGARIERRKDA"
     gene            2458..3294
                     /locus_tag="PK937_15255"
     CDS             2458..3294
                     /locus_tag="PK937_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33980.1"
                     /translation="MDPSARLIHTLIRQRSVVDAVDAGLALCRNRYYDLCVTTLIVFV
                     PLYSLKYVLFVLDLIPFERVFNSFGFFYVQSYTPANIMVWLLDSAANTLCGAAICRYV
                     TMMAESHTLPIRHTKLLPWGELWPLLFNTFIAELIIIIGFGLCILPGIWTGVMFMFVT
                     PLITYEKLTSFSDIWTRSKYLSQNDWWRMLGFLLLTFLLLAILGLSLTYLFAAVYQLV
                     LGQLAWLSGQTADENLLTMIGGAVVSVLLLPMQMVFTVVLYFDIRARREGLDIERMVQ
                     KL"
     gene            3291..4394
                     /locus_tag="PK937_15260"
     CDS             3291..4394
                     /locus_tag="PK937_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33981.1"
                     /translation="MKHIFILWILAGTMAAHAGAMQSDTIQHIGAEAYQLKMQSIYQA
                     ADSLYRITGSDTENPAIQIMRQAEDELPVHYAVVYSYRMRMGGDSLYTGIMRVDNRYL
                     KRLMQQSIKPDGTSYRDALRTELLDRLYALDELAENGIILDSTDYRVVALRIAADKDF
                     QPEQKTNVFSYFIDKFFEWLRRLFPGNPDTPETKEQTNNADYGWLATILLYSIYAIGL
                     ALLVYIIYWAYKQYRRNTTADEMLPATATHSIIEPGESTDYEVHASLATQYAVSGDYR
                     KAIRHLYIALILELDARLILRFDKAYTINEYLTFAKNQSVAPELLRLLIQSSAQFEYF
                     WYGHQTTDRADFERLLLWYNEARNLYNTASHGK"
BASE COUNT         1158 a         1117 c         1038 g         1247 t
ORIGIN      
        1 gaaaactatc ttattccatt tgtacaaaaa aaaccgtcat cgcatgtggt tcgctacaaa
       61 accattcgca cgaccggtat tcccgaatcg aatttgtttg aaaccctgcg ttcgcaggtg
      121 caatcttatc gtgaacgttt ggatatcgct tttctcccca aactcaccgt gggtgtagat
      181 ttacgattta cagttcagaa tatgacggct gaaaaggccg attcgctctt gaacgaggcg
      241 gaacaagctt ttcgacaagc gatcgagtca acctatccgc atggcgtcta tggtgaagga
      301 acgatttccc ttgaagacgt tgtcgccgat ttactttttc gtcacaatct gaccatcgcc
      361 actgccgaat cctgcaccgg cggcttgatc gctcaccgct tgaccaatgt accgggcagt
      421 tcccgatatt ttatgcaagg tatcacgacc tatagcaatg ccgcaaaagt gcagcatgta
      481 ggcgttaccc agacgctgct ggatgaacat ggcgccgtaa gtgaacccgt tgtccgtgcc
      541 atggccgaag gtatgcgccg cgttgcacaa accgatatcg cgatcgctac aacaggaatt
      601 gccggcccca caggtgcgac cgaagccaaa ccgctcgggc tggcgtacgt cgcgctggcg
      661 accgcctcgg gcacgcatgt tttcaaacat agcccgcttc ccttttccat cgaacgactt
      721 gcttttaaag aacgattatc ccaatttgcg ttagatcgtt tacgtcacta cttgatcggt
      781 ttatcagaat gatcatttgc aaagtcacag gctctgttgt ttcgacacat aaaaacgagt
      841 atttgcacgg tcataaaatc atggtcgtac agcctgtgga tctcgaaaaa aatcatatcg
      901 gcagtgatat gcttgctctg gacgaagtca gtgccggtgt cggtgacctt gttctcgtta
      961 tgcgtgaagg cggttctgcg cgtattgttt tcggtcaaaa aaaactgccc gtacaagctg
     1021 ttatcgtcgg cgtgattgac gattacgact atcgctaagc atacctcctt gtcccctatt
     1081 tcgttgaagt tagctctgaa atttcctatc cttgtcctaa cactttactt tattcaattt
     1141 taactcacct ctatagttat ggaaaaattt atagtcgaag gtggtcatcg tcttagcggc
     1201 acctttcaac ctgcaggaaa taaaaacgaa gcgctgccgg ttattgccgc gtgtatgctg
     1261 acggacgaac cgatcatttt aaaaaatatg ccggatgtca gcgatgcgcg ccacatgctg
     1321 gatattgcag ccaatatcgg cgctgaagta acccggcttg gcccctcgga atggaaagta
     1381 caagctaaaa gcattcgtac gcacgaaccc gattatgcac tggcgtcgga aatccgaggt
     1441 tcatttttat tcgccggatc gctactcggt cgtcatggcc gcgtgagttt accttcaccc
     1501 ggcggcgaca aaataggacg acgacgcctt gacacgcata tcctggcttt gcaaaaactc
     1561 ggcgcacaca tggatttggg aagaaagttt tatacgatgg aatcccgcgg ccgactaatc
     1621 ggcgaggata ttttcctcga tgaggccagc gtgatggcta cggaaaatgc cgtactggcc
     1681 gcggtattag ccaaaggcac cacgatactc aataatgccg ccggtgaacc gcatgtgcag
     1741 ggcctttgcc gaatgctcaa tgcgatgggc gcaaaaataa gcggtatcgg aagcaatata
     1801 ctcaccatcg aaggggtaga taagctgcac ggttgcaccc atacgatcac caacgatcac
     1861 attgaaatcg gcagtatcat cggtttagcc gcggcgacca aaagtgaaat tcttatcaaa
     1921 aatacgacac cccgcaatct gcgcatgata cttttagtat ttgaacgcct cggcgtgcgt
     1981 gtcgaaattc gcggcgaaga tttatttatc cccggcggac aagagcctga aatcatgtcc
     2041 gacatggatg gcgccatccc caaaattgat gatgctccct ggccgggatt tcctgccgat
     2101 cttacgagta tcatggttgt tacggccact cagtgcaaag gtatggtact gatatttgaa
     2161 aaaatgtttg aaagccggtt attttttaca gataaactta tctcaatggg cgctcgtatc
     2221 gtcctatgcg acccacaccg tgccgttatc aatggcccta caccgttgca tggcgagctg
     2281 ataaccagtc cggatattcg tgccggtatg tcacttctga tcgccgcgct ttgtgccgaa
     2341 ggcactacca cgatccaaaa tattcgccaa ctggatcgcg ggtatgaacg gattgatgag
     2401 cgactcaatg cactgggagc acgtatcgaa cgacgcaaag atgcttaacc tcgaactatg
     2461 gatccatctg cgcgacttat acatacatta atacgccagc gctccgtggt ggacgccgtg
     2521 gatgccggtt tggctctgtg ccgaaatcgt tattacgatt tatgtgtcac cacgttgatc
     2581 gtgttcgtac cgctttattc cctgaaatac gttcttttcg tgttggattt aattcctttt
     2641 gaacgcgtct tcaattcgtt cggctttttc tatgttcaat cgtacacacc ggccaatatc
     2701 atggtatggc ttttggacag cgctgccaat acgctatgcg gtgctgcaat ctgccgttat
     2761 gtgacgatga tggccgagtc gcatacttta ccgatccgac ataccaaact tttgccgtgg
     2821 ggtgaattat ggccgctcct gtttaatacg tttatagcgg aactaatcat tatcatcgga
     2881 tttggcttat gtattttacc cggcatttgg accggggtga tgtttatgtt tgtgacgcct
     2941 ctgatcacat atgaaaaact aacttctttt tcagatattt ggacgcgttc aaaatatttg
     3001 tcgcagaacg actggtggcg aatgcttgga tttttattgc tcacgtttct tttacttgcg
     3061 atactcggtt tatctttgac ttaccttttt gctgccgtgt atcagttggt tctcggtcag
     3121 ttagcctggc ttagcggtca aacagccgac gaaaatctgc tgactatgat cggcggcgcc
     3181 gtcgtatccg tactgctgtt accgatgcaa atggtattta ccgttgtttt gtattttgac
     3241 atccgtgcac gccgtgaagg tttagacatc gaacgaatgg ttcaaaaact atgaaacata
     3301 tttttattct ttggatatta gccggtacga tggccgcaca tgccggcgca atgcaatccg
     3361 atacgataca gcacatcgga gcggaagcct atcaattgaa aatgcagtcc atttatcaag
     3421 cggctgattc gttgtatcgc ataaccggct cagatacaga aaaccctgcc atacaaatca
     3481 tgcgccaagc cgaagacgaa ttaccagttc actatgccgt cgtatatagc taccgtatgc
     3541 gtatgggcgg cgattcactt tataccggca tcatgcgcgt tgacaaccgt tatttgaagc
     3601 gtttgatgca acaaagtatc aaacccgacg gaaccagtta tcgcgatgca ttgcgaacag
     3661 agttgctcga ccgcctgtat gctttggacg aactggctga aaacggaatt attttggata
     3721 gcaccgatta ccgtgtagtc gctttgcgaa tcgccgcgga caaagatttc caacctgaac
     3781 aaaaaaccaa tgtcttttca tatttcattg ataaattttt tgaatggttg cgtcgcctct
     3841 ttcctggcaa tcccgataca ccggaaacaa aagaacaaac caacaacgcc gactacggat
     3901 ggctggctac gattttgctg tacagcatct atgcgatcgg tttagctttg ctagtctata
     3961 ttatttattg ggcgtataaa caataccgcc gtaatacaac ggccgatgaa atgttaccgg
     4021 ccacggcgac acatagcatc atcgaacccg gcgaatctac ggattacgag gtacatgctt
     4081 cattggcaac acaatatgcg gtttccggag attaccgaaa agcaatccgc cacttatata
     4141 ttgctttgat tttggaactt gacgcccgat tgattttacg gtttgataaa gcctacacga
     4201 tcaacgaata tcttacgttt gccaaaaacc aatccgttgc accggagctt ttgcgattgt
     4261 tgatacaatc cagcgcacaa tttgaatact tttggtacgg ccatcaaaca acggatcgcg
     4321 ctgattttga gcgcttgtta ctatggtata acgaagcaag gaatttatat aatacggcaa
     4381 gccatggcaa ataatcgttt attcttgagc ctcagcattt tttgcggcct agctattttg
     4441 cttcttcttt ttttttccga cccgggcgcc actatacata ccgattcccc tacaagttat
     4501 tctgttcaac cgggcggcac aaaaatattt tacaagcttc ttaccgacaa cggctacaac
//
LOCUS       DAOQGF010000185         4518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_10220_length_4518_cov_5.389872, whole genome shotgun sequence.
ACCESSION   DAOQGF010000185 DAOQGF010000000
VERSION     DAOQGF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_4518_cov_5.389872"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..809
                     /locus_tag="PK937_15265"
     CDS             126..809
                     /locus_tag="PK937_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33982.1"
                     /translation="MLKNLILSAFVLSLISCGKPSNIEEFQRLVQKVSKKNEEIGSIN
                     MEIAEAVRKYNESRKADEQPIVLPDSMMGLNKEQLKLIQDMISKEQDISTKGMLTQII
                     DKNKTIEKLNADLEDMKAKLPRPVVVKAGDTHHKIGYDFLTKEKGVDPKRANELLEQS
                     FLADDLLPGFNVWVYYNDDVFGTFVNQGNVRISPNQFSRIIRKKQMDDAREAGRQDAT
                     EKPAEPAAK"
     gene            complement(901..2406)
                     /gene="accC"
                     /locus_tag="PK937_15270"
     CDS             complement(901..2406)
                     /gene="accC"
                     /locus_tag="PK937_15270"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNH33983.1"
                     /translation="MSITHRTIKKILIANRGEIAIRVIRACREMNIKTVAVFSEADRT
                     AAHVRLADEAYCIGEPPSNKSYLRMDKIIEVAKKSGADAIHPGYGFLSENEDFADLVI
                     QNGIIFIGPSSEAMAQMGSKTAARTLAKKMNVPTVPGTESGIKDKNEAITIAEKIGFP
                     VLIKAAAGGGGKGMRVVQKIEELSEAIDRARGEALNAFGDDTVFIEKYVTKPRHIEIQ
                     IIGDQHGNMVYLGERECSIQRRHQKVIEEAPSAIVTPEMRKRMGESAVRLAQAVNYYN
                     AGTMEFLVDADMNFYFLEMNTRLQVEHPVTEMVTGIDIVKEQIRIASGEPLSFRQEDI
                     RIHGHAIESRIYAEDCENNFAPSIGRIEHLEPSYGPGIREDSGVFEGDTIQIYYDPMI
                     SKLAAWGPTREEAINRMRRSLREYAIVGVETTIPFCLFVMENEKFRSGDFDTSFVGKE
                     FTPEKLQHKEEKMAAIAAAVHDFAKKRDMKISTDALTASSPKSSRWKRNRN"
     gene            2524..3285
                     /locus_tag="PK937_15275"
     CDS             2524..3285
                     /locus_tag="PK937_15275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH33984.1"
                     /translation="MSASTAYPEFVARFYDTVYAKIRTSIDHDFYVEETTRAQGSVLE
                     VGVGTGRLFMEALRAGADIYGLDASATMIRTLQAKLDAKEKQRVSVADVRDFTMDRRF
                     ALIVAPFRVFTHLRTVDEQLAALACIKDHLEPGGTLILDVFNPDPVLCSQGRESTLDF
                     DGEWAPGQRLKRFTTVVPDFMHQINHVTMRYVWDENGTTHDESWSFRVRYYFRFELEH
                     LIYRSGLALETMYGGFDRTAFDENSKELIAVCKSN"
     gene            complement(3292..4176)
                     /locus_tag="PK937_15280"
     CDS             complement(3292..4176)
                     /locus_tag="PK937_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNH33985.1"
                     /translation="MTWKTVLLYFFIVLFSAAIGGGIVGYYAYEKYYRPMDLDLWKTR
                     NALAESHAQLQAIYPEYEHAKQFADSLQTFIPLQSGLNYPMESERQYYYHDKMFNIIY
                     DTKRYLEQKRRKQESHFLELVDHVMPHEKHLRMILDSIGVKTNDTLHLEKNAQAIMDF
                     VQCIPYQSENRAYTKMPLETLYEGGGDCEDLAVLAYSLLTTAGIEAVLVVPTRTIASI
                     DHATVAIHGNFTGQYIEHNQKRFFIAESAGTTDARKPKRFKIGRAEKYNISLVLGDSL
                     EVAKHEVDWIIKEKLRTR"
     gene            complement(4178..>4518)
                     /locus_tag="PK937_15285"
     CDS             complement(4178..>4518)
                     /locus_tag="PK937_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNH33986.1"
                     /translation="PLTITEYEEWGNPNDPAYFDYMMSYAPYENVKRQAYPHILVTAG
                     LNDPRVSYWEPAKWVAKLRRMKTNRNDLLMRTHMDFGHSGASGRYDYLLEIAFDYAFI
                     IEKTKPSGRS"
BASE COUNT         1143 a         1020 c         1109 g         1246 t
ORIGIN      
        1 ttactaacgt acagtcaggt gatttattcc gtgattaaat ttccttgact ttggcggggc
       61 taaaacctac aatcgcgcgt tagtgaaccc taaacccaaa tatcatgtat cgaggagaaa
      121 aaaccatgtt aaagaatctc atcctgagcg cgtttgtgct tagcctgatc agttgcggca
      181 aacccagcaa catcgaagaa tttcagcgct tagtgcaaaa agtcagcaag aaaaacgaag
      241 agatcggttc catcaatatg gaaattgcgg aagccgttcg taaatacaac gagtcccgca
      301 aagccgacga acagcctatc gttttgccgg attctatgat gggattgaat aaagagcagc
      361 tcaagctgat tcaggacatg atttccaaag agcaggatat ttcgaccaaa ggcatgctca
      421 cccagattat tgataaaaat aaaaccatcg aaaaactcaa cgcggatttg gaagacatga
      481 aagccaaact gccgcgtccc gtcgtcgtca aagccggtga tacgcatcac aaaatcgggt
      541 atgatttttt gacgaaagaa aaaggtgtag atcctaagcg tgcgaatgaa ttgctggaac
      601 aatccttttt agctgatgat cttttgccgg gctttaatgt atgggtgtat tataatgatg
      661 atgtgttcgg tacgtttgtc aatcaaggca acgtgcgtat ttcgcccaat cagttcagcc
      721 gcattatccg taaaaaacaa atggatgatg cccgtgaagc cggtcgtcag gacgctaccg
      781 aaaaacccgc agagcctgca gccaagtaat atttgaaaat gaagtaaaaa aaggcggccg
      841 aagagccgcc ttttttgtta tgctaaatac attctgttat gcataccgca aaaacccatg
      901 ttagttgcgg tttcgcttcc atcggctgga tttgggagag gaagccgtca atgcgtccgt
      961 gctgattttc atatcacgtt tttttgcaaa gtcatgaaca gcagcggcaa tggcggccat
     1021 tttttcttct ttatgctgaa gcttttccgg agtgaattcc tttcccacga aactggtatc
     1081 aaaatcgccg gagcgaaatt tttcgttttc cattacaaaa agacaaaacg gaatcgtggt
     1141 ttcgacgccg acaatcgcat attcacgcaa ggaacgtcgc atgcggttaa tagcttcttc
     1201 acgtgtgggt ccccaggccg ctaatttgga tatcatcgga tcataataga tctggatggt
     1261 atcgccttcg aaaacgccgc tgtcttcgcg aatgcccggg ccgtacgacg gttccaaatg
     1321 ttcgatgcgg ccgatggacg gtgcaaaatt attttcgcaa tcttcggcgt aaatgcggct
     1381 ttcgatagca tggccgtgta tgcggatgtc ctcctgccgg aaggaaagcg gttcgccgga
     1441 tgcaatgcgt atctgctctt tgacgatatc aatgccggtg accatttccg tgacgggatg
     1501 ctcgacttgc aaacgtgtat tcatttcgag aaaataaaaa ttcatatccg catccacgag
     1561 aaactccatc gtaccggcat tgtagtaatt gacggcttgc gccaaacgaa cggcggattc
     1621 gcccatacgt ttacgcattt ccggtgttac gatggccgag ggcgcttctt cgatcacttt
     1681 ctggtgacgg cgctgtatgg agcattcgcg ttcgccgaga tacaccatgt tgccgtgttg
     1741 atcgccgatg atttggattt cgatgtgccg cggtttagtg acatattttt cgatgaaaac
     1801 ggtatcgtca ccgaaagcat tgagcgcttc gccgcgtgcg cgatcgatcg cttcgctgag
     1861 ttcttcgatt ttttgaacca cgcgcatgcc tttaccgccg ccgccggccg ccgccttgat
     1921 caaaacagga aagccgattt tttcggcgat cgtgatggct tcgtttttat ctttgatacc
     1981 ggattcggta ccgggcacgg tcggaacatt catttttttg gccagcgttc tggccgcggt
     2041 tttactgccc atctgcgcca tcgcttccga cgacgggccg ataaaaataa taccgttttg
     2101 aatgaccaaa tcggcaaaat cttcattttc actgaggaaa ccgtatccgg gatgaatcgc
     2161 atccgcgccg gattttttgg cgacttcgat gattttgtcc atgcgcagat agcttttatt
     2221 ggacggcggt tcgccgatgc aataggcttc gtccgctaaa cgaacgtgcg ccgccgtacg
     2281 atccgcttcg gaaaaaacgg cgacagtttt tatattcatt tcgcggcatg cccggatgac
     2341 gcgtatggct atctcaccgc gattggcgat gagaattttt ttgatggtgc gatgggttat
     2401 gctcataagt attatagttc tttcagaaaa gcatttttga taaattgata aaattatcct
     2461 tgcacccgta aatttaaccc aatagttttc attattcaat agccaactcc aaaggagata
     2521 cctatgtcgg cttctacggc ttatcctgaa tttgtagcac gattttatga tacggtgtat
     2581 gccaaaatac gtacatccat agatcatgat ttttacgtcg aagaaacaac ccgcgcgcaa
     2641 ggttctgttt tggaagtcgg tgtgggaacg ggacgacttt ttatggaagc gttgcgtgcc
     2701 ggtgcggaca tctacggact ggatgccagc gcgacgatga ttcgcacctt acaagccaaa
     2761 cttgatgcca aagaaaagca acgcgtgagt gtcgcggatg tgcgcgattt tacgatggat
     2821 cgccgttttg cgttgattgt cgcgccgttt cgcgttttca cgcatcttcg tacggtggat
     2881 gagcaacttg cggcgttggc ctgcatcaaa gatcatttgg aacccggcgg tacattgatt
     2941 ttggatgttt ttaatccgga tccggtattg tgctcacaag gacgtgaatc cacgttggat
     3001 tttgacgggg aatgggcgcc cggccaacgt ctcaaacgat ttaccaccgt cgtcccggat
     3061 ttcatgcacc aaatcaatca tgtgacgatg cgttatgtct gggatgaaaa cggtacgaca
     3121 cacgatgaat cgtggtcatt ccgggtacgt tattatttcc gatttgaact ggagcatttg
     3181 atttaccgga gcggattggc gctggagaca atgtacggcg gatttgatcg tacggccttc
     3241 gatgaaaact caaaagaact gattgccgtt tgtaagagca attgaggttg tttaccgggt
     3301 tcgcagtttt tctttgatga tccagtccac ttcgtgtttg gctacttcga ggctgtcacc
     3361 gagtacgagc gaaatgttgt atttttccgc gcgaccgatt ttaaaacgtt tgggtttgcg
     3421 tgcgtcggtc gtgccggcgg attcggcaat aaaaaaacgc ttctgattat gttcaatata
     3481 ttggcctgta aaatttccat gaatagcgac cgttgcatga tctatggaag cgatcgtacg
     3541 tgtgggtacg accagaaccg cttcaatgcc ggccgtcgta agcagcgaat acgctagaac
     3601 agctaaatct tcacaatccc ctccgccttc gtacaacgtc tccagcggca ttttggtgta
     3661 agcacggttt tcggattgat acggtataca ctgaacgaaa tccatgatgg cctgtgcatt
     3721 tttttccaga tgaagcgtat cgtttgtttt tacgccgata ctgtcgagga tcatgcgcag
     3781 atgtttttca tgcggcatca catgatctac taattccaga aaatgcgatt cctgtttgcg
     3841 gcgtttctgc tccagataac gtttggtatc gtagatgata ttaaacattt tgtcgtggta
     3901 ataatactga cgttcgcttt ccatcggata attaagtccg ctctgcagtg gaatgaatgt
     3961 ttgcagcgag tcggcaaatt gtttcgcgtg ttcgtattcg ggataaatag cctggagttg
     4021 cgcatgggat tcggccaatg cgttgcgtgt tttccacaaa tcaagatcca tagggcggta
     4081 atatttttca tacgcataat aaccgacgat cccgccgccg atggctgccg aaaacagtac
     4141 gataaaaaaa taaagtaaaa ctgttttcca cgtcatatca agaacgtccg gacggtttgg
     4201 ttttttcaat aataaaagca taatcgaaag cgatctctaa caaataatca taacgacccg
     4261 aagcgccgct gtggccaaaa tccatgtggg tgcgcattag aaggtcgttg cgattggttt
     4321 tcatacgccg cagtttggca acccacttgg ccggctccca ataacttacg cgcggatcat
     4381 tgagcccggc cgttacgagg atatgagggt acgcttgacg tttgacattt tcgtaaggcg
     4441 cgtaagacat catatagtca aaataagcgg gatcattggg gttgccccat tcttcgtatt
     4501 cggtgatggt cagaggca
//
LOCUS       DAOQGF010000186         4515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_10233_length_4515_cov_5.377354, whole genome shotgun sequence.
ACCESSION   DAOQGF010000186 DAOQGF010000000
VERSION     DAOQGF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10233_length_4515_cov_5.377354"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1052)
                     /locus_tag="PK937_15290"
     CDS             complement(375..1052)
                     /locus_tag="PK937_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33987.1"
                     /translation="MKKIAWITANIGGIDTPRDEAPQLVDGYEVKRIYVTEYDAAATG
                     YNAPNPRMAAKWYKCHGWKLPEAKDCEIICWTDANIELKPGALQFWVNRLNTGNTEIL
                     VQEHHHRKTMREEAVFIYAQRETDRYLAARYAGYDFEPEVECWHEDNLHNSTLLCSNI
                     FSYKNAHQAQRVMNHWWECILQYGHYDQFSLSALVFLYLYRQNLYDGHEYYNNPYFTH
                     HNHRVIM"
     gene            complement(1049..1318)
                     /locus_tag="PK937_15295"
     CDS             complement(1049..1318)
                     /locus_tag="PK937_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33988.1"
                     /translation="MKLIFCEAPNNNISLLIYKCAENDGISGTNTYKIYNGEKKMCYI
                     MDINGYGEKDIRRAWDAIKNDLSYDDLNHGTNIKIKFMLAIAKEL"
     gene            complement(1315..1887)
                     /locus_tag="PK937_15300"
     CDS             complement(1315..1887)
                     /locus_tag="PK937_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNH33989.1"
                     /translation="MTFPESKLAHKYLDGLRGIEIGASAHNPFGLNTLNVDYRKHGPG
                     DMWYDEQMKQCGQCASVDVVADARYLPFSDGEFDFVLSCHSLEHIFDPISALCEWKRV
                     ATKYIFIIIPHRDAAPDDAGRPVTTLSELQDRFNAITPYDYTDTHHSVWDSVAFLELL
                     KYLNFEIVEWQAVDDKVGNGHAYVIKLSKI"
     gene            complement(1884..2240)
                     /locus_tag="PK937_15305"
     CDS             complement(1884..2240)
                     /locus_tag="PK937_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33990.1"
                     /translation="MESAKNDVRCKILVDDGVIVLATNNGVRIPKQLDISISQYLKKS
                     PIVVVSVLVDSEHIIHSEPKCTYNKDTKEILFNGEKIPGSMVVSFTEANETICAMVTF
                     KLIADAVDTKINSIKL"
     gene            complement(2250..3947)
                     /locus_tag="PK937_15310"
     CDS             complement(2250..3947)
                     /locus_tag="PK937_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNH33991.1"
                     /translation="MLEKYHQYIEDVLSGKELVCRLTRLAVERQVKDLERQEQDDYPY
                     YFDENAAATVIRFVKSLRHVAGKWAGQLFDPEPDQVFKYAVIFGWKNKETKKRRFRRV
                     YMEVARKNGKSFDASVIELVGLLLDGEARAEIYSAATTRDQAGIVFEVSKDILRRLIK
                     DSPAIEKRIKVYAHSIVNVSTGGRIGKVSSDAGKLDGLSPSMWIVDEYHEHPTDKVLK
                     VGETGMGAREQPLSVVITTAGFNITGPCYDLRKTAVQILEGLKTDDAFFSVIYTIDED
                     DDWNDEAVWKKSNPHIGISPSWEFMRAEYTRAKNEGSSAEVQFLTKNLNVWTSSKATW
                     VQDDKWMKCATPVDLEALDGARCWAGLDLGEVFDFTAFCLVFGPETENDTYKMAWWFW
                     IPEEAAALFESRGHGAYRQWADDGHITITPGNVVDLDYVISDIADIGKRYKIETVGID
                     PWGSKTNVLKLQEIGMSITEVRQGYRTLSPPMKDFERLIMTQQIAHGGNPVMRWMMSN
                     VNAVRDPAGNIKPDRSSKENKIDGVVAAIMALGLAIDGAKPAQLSYLFDDETDLITI"
     gene            complement(3947..4462)
                     /locus_tag="PK937_15315"
     CDS             complement(3947..4462)
                     /locus_tag="PK937_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HNH33992.1"
                     /translation="MAKGRPPKSDEELKRNGSYNTTHHKDRVKLKELDGVPPSPFEDE
                     YRTSRWVHYCELMQETHHLTQRHLDGIELMCNLDSDLIEVSENIKRTGITFTTETGQI
                     KANPAVGIKLNIQSQMMRMYEQFGMTPRSSMNMKVPPDSGVKDTDPFEEVLAMMARNE
                     TQKPAVKRAKA"
BASE COUNT         1079 a         1168 c          936 g         1332 t
ORIGIN      
        1 attgaaatac attctcccat ccggcccact ttgccgcaag gtcaaagcct ccaattccag
       61 aaaaaagtga tccgtgtttc attttgttga ttttaatcgg ctaaccccgc tttcccgtca
      121 ataatcccta aacggtcaaa agcgggaaag cagcagttat tcgcgctcag aaattatagc
      181 accattctcc ctgtcaaaga ttagggcgta caccgaccgc taaacgcggc gcggcgcaca
      241 gccgaagtta gacacaatct attatatcca tttcggaggc tcctcatctc gattatccat
      301 atcgccacca tggaaaaacc atgcaacggc gaatatgaaa acaccggcaa ttaatacgaa
      361 tacgattttc atggctacat aattactcgg tgattgtgat gggtgaaata cggattgttg
      421 tagtattcgt ggccgtcgta gaggttttgg cggtataaat aaagaaacac cagcgccgac
      481 aaagaaaatt gatcgtagtg gccatactgc aaaatacatt cccaccaatg attcattaca
      541 cgctgagcct gatgtgcatt tttgtaagaa aaaatgtttg agcaaagtag cgtcgaattg
      601 tgtaaattat cctcatgcca acactcaacc tctggctcaa aatcataccc ggcataccgc
      661 gccgccaaat accgatctgt ttcccgttgc gcgtagatga aaacggcctc ctctcgcatg
      721 gttttgcgat ggtggtgttc ctgtaccaat atttcggtat tgccggtgtt tagccgattc
      781 acccaaaatt ggagcgcacc gggttttagt tctatattcg catccgtcca acaaataatc
      841 tcgcaatcct ttgcctccgg taatttccat ccatggcact tgtaccactt tgccgccatg
      901 cgcggatttg gtgcgttgta tccggtcgcc gccgcgtcat attcggtaac gtagatacgt
      961 ttaacttcgt agccgtctac cagttgcggc gcttcatccc gtggcgtgtc gatgccgcct
     1021 atgtttgcgg taatccaggc tatttttttc atagttcctt cgcaattgca agcatgaact
     1081 ttatttttat attagtgcca tgattaagat catcgtaaga taaatcgttt tttattgcat
     1141 cccatgctct tcttatatct ttttcaccat atccgtttat atccataata tagcacattt
     1201 ttttttctcc attataaatt ttatatgtat ttgtaccgct tattccatca ttttccgcgc
     1261 acttatatat aagcagggaa atattattgt ttggcgcttc gcaaaaaata agtttcatat
     1321 tttggatagt ttgataacgt atgcgtgtcc gttccctacc ttatcatcaa ccgcctgcca
     1381 ttccactatc tcaaagttta ggtatttcaa caattcaaga aacgccactg aatcccaaac
     1441 agaatgatgt gtatccgtgt aatcgtatgg tgttatagcg ttgaacctgt cttgcaattc
     1501 agacaaagtt gttaccggcc gtccggcgtc atcgggtgcc gcgtcgcggt gcgggataat
     1561 tatgaaaatg tattttgtag ccactcgctt ccactcacac agtgcggata tagggtcgaa
     1621 aatatgctcc aggctgtgac acgaaaggac aaaatcaaat tctccatcag aaaacggcaa
     1681 ataccgagca tcggccacca cgtcaacgct tgcgcactgc ccgcactgtt tcatttgctc
     1741 atcgtaccac atatcgcccg gcccgtgctt ccggtaatcg acattcagcg tattaaggcc
     1801 gaacgggttg tgtgcgctgg ctccgatttc gatgccgcga aggccgtcaa ggtatttatg
     1861 tgcaagtttt gattctggaa aggtcataat ttaatcgaat tgattttagt atctacggca
     1921 tctgcgatta atttgaatgt taccatagca caaatcgttt cattggcttc cgtaaaagat
     1981 acaaccatag acccaggtat tttttctcca ttaaataata tttctttagt gtctttattg
     2041 tacgtgcatt ttggctctga atgtataatg tgttcagaat ctactaacac ggaaacaact
     2101 acaataggac tctttttaag atattgagat attgaaatat ccaactgttt cggtatccgt
     2161 actccattat tagtagcaag cacaataaca ccatcatcaa ccaatatctt acacctcacg
     2221 tcattttttg cagattccat tttttgattt taaatcgtta tcaaatctgt ttcgtcatca
     2281 aaaaggtatg aaagttgcgc cggtttcgcg ccgtcgatcg caaggcccag ggccattatc
     2341 gcggccacca cgccgtctat cttgttttct ttgctactcc gatctggctt aatgtttccg
     2401 gcaggatcac gaaccgcgtt tacattcgac atcatccacc gcattaccgg atttccgcca
     2461 tgtgcaattt gctgcgtcat tatcaggcgc tcgaaatctt tcatcggcgg gcttaatgtc
     2521 ctgtaacctt gccgcacttc ggtaatgctc attccaattt cctgcaattt cagcacattc
     2581 gtttttgatc cccacgggtc aatacctacc gtttcaattt tgtatcgctt gccgatgtcg
     2641 gcaatgtcgg agatcacata atcaaggtct accacgtttc ccggcgtaat cgttatgtgc
     2701 ccatcgtccg cccattgccg gtatgctcca tgcccgcggc tttcgaataa ggcggcggcc
     2761 tcctcaggga tccaaaacca ccatgccatt ttgtaggtgt cgttttctgt ttcaggcccg
     2821 aacaccaggc agaacgccgt aaagtcgaac acctcgccaa ggtcaaggcc cgcccaacac
     2881 cgcgcaccat caagcgcctc caagtccacc ggcgtggcgc atttcatcca cttgtcatcc
     2941 tggacccatg tcgccttact gcttgtccaa acgttgaggt tctttgtcaa gaattgcacc
     3001 tccgcgctgc tgccttcgtt cttcgcccga gtatactcag cccgcataaa ttcccaagat
     3061 ggagaaatac cgatgtgcgg gtttgatttc ttccaaaccg cttcatcgtt ccaatcgtca
     3121 tcttcgtcga tggtgtatat caccgaaaag aaagcgtcat cggttttcaa gccctccaaa
     3181 atttgcaccg ccgttttccg aagatcgtag cacggaccgg taatattaaa tcccgcggtg
     3241 gtgattacca ccgacaaggg ttgctccctg gcacccatgc cagtttcgcc tactttcaga
     3301 accttatcag tcgggtgttc gtggtattcg tctacgatcc acatggacgg agaaaggccg
     3361 tctaatttcc cggcatcgct cgatactttc ccgatacggc cacccgtgga aacgtttaca
     3421 atggagtgcg cgtacacttt tatcctcttt tcgattgcag ggctgtcttt tataagccgc
     3481 cgcaaaatgt cctttgaaac ttcgaacacg ataccggcct gatccctggt cgttgcagcg
     3541 ctgtaaattt cagcccgcgc ttcgccgtcc aataacaaac ctaccaattc aattacggac
     3601 gcgtcgaatg atttgccgtt tttgcgggca acctccatat aaacccggcg aaaccgtcgc
     3661 tttttagttt ccttgttttt ccatccgaat attaccgcat acttaaacac ctgatccggc
     3721 tcagggtcga ataactgccc ggcccatttg ccggcaacgt ggcgaagcga tttcacaaaa
     3781 cggattaccg tggccgccgc attttcgtcg aagtagtacg gataatcatc ctgctcctgc
     3841 cgctccaagt ctttcacctg gcgctccacc gcaaggcgcg taaggcggca aacaagttct
     3901 ttgccggata acacatcttc gatatattgg tggtactttt cgagcatcag gcttttgcgc
     3961 gttttaccgc tggtttttgt gtttcgttgc gggccatcat tgccaaaacc tcctcgaaag
     4021 ggtcggtatc ttttacgccc gaatcaggcg gaactttcat gttcatactg ctgcgcggtg
     4081 tcattccaaa ctgctcgtac atccgcatca tttgcgattg aatgttcaac ttgataccga
     4141 ccgccggatt tgctttgatc tgccctgttt cggtggtgaa cgttatccca gtgcgtttta
     4201 tattttccga cacctctatc aaatcggaat ccaggttaca cataagttcg atcccgtcga
     4261 ggtgccgctg cgtaagatgg tgcgtttcct gcatgagttc gcagtagtgg acccaccttg
     4321 aggtccggta ttcatcttcg aaaggagaag gaggaacacc gtctaactct ttgagcttta
     4381 cacggtcttt atggtgcgtc gtgttgtacg atccgttccg ttttaactcc tcgtctgatt
     4441 ttggtggtct accttttgcc atttttagac ttgcctaaat ttaaagggct attttttaaa
     4501 ttgtatgcgt ggaag
//
LOCUS       DAOQGF010000187         4488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_10308_length_4488_cov_3.827882, whole genome shotgun sequence.
ACCESSION   DAOQGF010000187 DAOQGF010000000
VERSION     DAOQGF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10308_length_4488_cov_3.827882"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PK937_15320"
     CDS             <1..355
                     /locus_tag="PK937_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNH33993.1"
                     /translation="ILFIDARNHGFLVNRRTRELSEDDVKLIAGTYHHWRNSDKKYKD
                     VQGFCKSSTIEEVKALNYVVTPGRYIGLPDDEDDFNFAERFTSLKKELEKQMAEEADL
                     NEKIRVNLKKIKVSP"
     gene            403..1911
                     /locus_tag="PK937_15325"
     CDS             403..1911
                     /locus_tag="PK937_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNH33994.1"
                     /translation="MTKLRVFISSVQSEFTEERQMLYDYLTADALLGRFFEPFIFEKL
                     PASDIHPTAAYLEEVKNSDIYLGILGKLYGFENKDGVSPTELEFNLASAEHKTRLIFI
                     RHVSDDDRHPKEKQFIQKAQDVVVRNKFTSASELKTAVYASLVKLLEDKELIRSGPFD
                     ATICKGATLDDIEPERIEWFVQTAQSKRGFPLSANVPSDEILTHLNLLRNGGLSNAAV
                     LLFGKQPQRFFITSEIRCAHFHGNEVAKPIPAYQVYKGDVFQLVDQAVDFVLSRIDVS
                     VGDRSASVDVPVKYEIPRSAVTEAIVNAIAHRDYTSNGSVQVMLFRNRLEVWNPGHLP
                     YHLSVSKLKQTHGSYPSNPLLADPMFLAGYIERIGTGIPDMIKTCIEAGLKEPSLIQE
                     ESFKTILWRKEEAAEQVTDQPTDQVTDQPTDQVKRLISITLEPPSSVRELMYLLDLKH
                     RPSFMQNYLNPALDSGLMEMTLPDTPNSPNQKYRLTAKGKSLKIKLKQAKKR"
     gene            2055..3155
                     /locus_tag="PK937_15330"
     CDS             2055..3155
                     /locus_tag="PK937_15330"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNH33995.1"
                     /translation="MSEWKEHRLGDVIMFQRGHDLPRTSMKDGKYPVAGSNGIIGYHS
                     EYTTKGPGVTIGRSGSIGAPNYYESDYWAHNTVLYVKDFKDSDPKFIYYFLSTFDFMQ
                     FNAGSAVPTLNRNHIHELNVLIPGTNEQRAIASILSSLDDKIDLLHRQNQTLEALAET
                     LFRQWFVEEADEGWEMGRLADFGPIVCGKTPSKKIDSYFGGNIPFIKIPDMHGKTFVF
                     DTADSLSQEGANSQPAKLIPPKSVCVSCIATVGLVSMNAFPSQTNQQINTIVPKKEEH
                     RYFLYFSLKTMKDDLLARASGGTATDNLNTGDFSNIEMPKPSDNLLKDFHKEVSSLFD
                     KIYSNQTQILKLTQLRDTLLPKLMSGEVRVRS"
     gene            3325..3804
                     /locus_tag="PK937_15335"
     CDS             3325..3804
                     /locus_tag="PK937_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33996.1"
                     /translation="MKLNEFGKIVSGEWERTTQMRPNIRTADFVVMPNHIHGILVIAD
                     DDIRRGTLQKTDVDGGGVMGRGVLQYAPTENTNPDDMVNAPFKSPSKTIGAIIRGFKS
                     ATTKRINQLRNTPRLPVWQRNYYEHIIRNDASYHQITEYIRLNPARWEEDRLFNQLK"
     gene            3931..>4488
                     /locus_tag="PK937_15340"
     CDS             3931..>4488
                     /locus_tag="PK937_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNH33997.1"
                     /translation="MKITESDIELLAIGHLEKLGYHYLYGPHIASDGESPERENYAQV
                     VLTGRLQAAIARINKNLPAPVQEQALKEVLRVATPDLLQTNENFHRFLTEGITLDVRH
                     EDGERGKTVWLVDFENPSNNEFVVCNQFTVIENNQNKRPDIVLFVNGLPLVVFELKNP
                     TDEKATVQKAFQQLQNYKTAIPSLFY"
BASE COUNT         1368 a          990 c         1017 g         1113 t
ORIGIN      
        1 gatcctattc atcgacgcgc gcaatcacgg ctttctggtc aatcggcgca cgcgggaact
       61 ctccgaagac gacgtcaagc ttatcgccgg aacgtaccat cactggcgca attccgataa
      121 gaaatacaaa gacgtgcagg gattctgtaa atcttcgacg atcgaggaag tcaaagctct
      181 gaactacgtc gtcacacccg gccgctatat cggcctgccg gatgatgaag atgatttcaa
      241 ttttgcagaa cggtttacgt cgctgaaaaa agaactggaa aaacaaatgg cggaagaagc
      301 ggatttgaat gagaaaatac gtgtgaatct aaaaaaaata aaggtaagcc cataaaaaac
      361 gaaacccgat agtttgaatt ttctaatatg ccagtgtaga gtatgacaaa actgagagta
      421 tttataagca gtgttcagag cgaatttacc gaggaaaggc aaatgctgta tgattattta
      481 acagccgatg cgcttttagg tcgtttcttt gaaccgttta tttttgaaaa attgcctgcc
      541 tctgatattc accctactgc cgcttatttg gaagaggtaa aaaattccga tatatacctc
      601 ggtattttag ggaaattata tggatttgaa aataaagacg gtgtttctcc cacagaattg
      661 gaatttaatc tggcttctgc cgaacataaa acgcggttga tttttatccg ccatgtgtcg
      721 gatgatgacc ggcatccgaa ggaaaagcaa ttcatccaaa aagctcagga cgttgttgtc
      781 agaaataagt ttacttccgc gtccgaactg aaaactgccg tctacgcatc gctggtcaaa
      841 ctcctcgaag acaaagaact tatccgatcg ggtccgtttg acgctacgat ctgcaagggc
      901 gcaactctgg atgatattga accggaaaga atagaatggt ttgtacaaac ggcgcaaagc
      961 aagcggggtt tccctctttc ggcaaatgtg ccttccgatg aaattttaac tcatctcaat
     1021 cttttaagga acggagggct gtccaatgcc gccgttcttt tgttcggcaa acagccgcag
     1081 cggttcttca ttacttcgga aatacgctgc gcgcattttc atggcaacga ggtcgccaaa
     1141 cccattcccg cgtatcaggt atacaaaggc gacgtattcc aattggtaga tcaggctgtg
     1201 gattttgtgc ttagccgtat cgatgtatcg gtaggagacc gaagcgcctc tgtggatgtt
     1261 ccggtgaagt atgaaattcc ccgttctgct gtaaccgaag ctattgtaaa tgccatcgct
     1321 caccgggatt acaccagcaa cggctccgtg caggtcatgc tcttcagaaa tcgtcttgag
     1381 gtatggaacc cgggacattt gccttatcac ctgagcgttt caaaactcaa acaaacgcat
     1441 ggttcctatc catcaaaccc tctgctggcg gatcccatgt tcctggccgg ttatattgaa
     1501 cggattggca ccggcatacc tgatatgata aaaacttgca tcgaggccgg tcttaaggaa
     1561 ccttcattga tacaggaaga atcttttaaa actattctgt ggagaaaaga agaggccgcc
     1621 gaacaagtta ccgatcaacc taccgaccaa gttaccgatc aacctaccga ccaagttaaa
     1681 cgattgattt caataacttt agagcctccg agttcggtca gagaattgat gtacctactt
     1741 gacctcaaac ataggccatc ttttatgcaa aattatctga atcctgcttt ggatagcggc
     1801 ctgatggaaa tgactttgcc ggatacacca aacagtccca accagaagta ccgtctcacg
     1861 gcaaaaggaa agagcttaaa aataaaatta aagcaagcga aaaaaagata ggtaacgcca
     1921 tgcacaaacg cagtgcatac gacagctaca atgcacaaaa aacgatcttt tctgtgcaat
     1981 caatcgtgca gcaccctgct gcacaaatgt aaacaaaagt gcaagtccgg cttgcacaaa
     2041 atccaggtga aataatgagt gagtggaagg aacatagact tggtgatgta ataatgtttc
     2101 agcgtggaca tgatctgcca agaacgtcaa tgaaggatgg caaatatccg gtggcaggtt
     2161 caaatggaat aatagggtac catagtgagt acacaacaaa ggggccgggt gttaccattg
     2221 gtagaagtgg aagtattgga gctccaaatt actatgaatc tgactattgg gctcacaata
     2281 cagttttgta tgtcaaagat tttaaagatt cagacccgaa atttatttat tattttcttt
     2341 caacttttga cttcatgcaa ttcaatgcgg gcagtgcagt tccaacttta aatagaaatc
     2401 atattcatga actaaatgta cttattcctg gtacaaacga acaacgcgcc atcgcctcga
     2461 ttctcagcag tcttgacgac aagatcgacc tgctccaccg ccagaatcaa accctcgaag
     2521 ccctagcgga aacgctcttc cggcagtggt ttgtggaaga agcggatgag gggtgggaga
     2581 tgggaaggct agctgatttt ggtccgatcg tttgcggaaa aacaccatca aaaaagatcg
     2641 attcatattt tggaggtaat attccattta ttaaaattcc cgacatgcat ggtaagacat
     2701 ttgtctttga tacggctgat tcacttagtc aggaaggtgc aaattctcaa ccagccaagt
     2761 taatacctcc aaaatccgta tgtgtttctt gtattgccac ggttgggctt gtttccatga
     2821 atgcttttcc aagtcaaacg aatcaacaga taaacacgat agtgcctaag aaagaagaac
     2881 acaggtattt tctttacttc tcgttaaaaa caatgaaaga tgatttgcta gcaagagcga
     2941 gtgggggcac tgcaactgac aatctgaata ctggtgattt ttctaatatt gagatgccaa
     3001 aaccaagcga taacttacta aaggattttc ataaggaagt ttcttcatta tttgacaaaa
     3061 tatattctaa tcaaactcaa attttaaagc tgacacaatt acgcgacacg ctattgccga
     3121 agctgatgag cggggaagtg agggtgagat catgaatgaa attcatatac atataaataa
     3181 aaacctgtga tggcatccgt caaaattcat aaccgccgat ccatacgatt gaaaggatac
     3241 gattacacac agccgggcgc gtattttatt accctttgcg cgcaggacag gatccattta
     3301 ttcggccaga tccgtcacgg cgaaatgaaa ttgaatgaat tcggcaaaat cgtttccggc
     3361 gaatgggaac gcaccacgca aatgcgaccc aatatccgca cggcggattt tgtcgtcatg
     3421 cccaatcata ttcacgggat attggtgatc gcggatgatg atattcgtag gggcacgttg
     3481 caaaaaacgg acgttgatgg tggtggtgtt atgggtaggg gcgtattgca atacgcccct
     3541 acggaaaaca ccaaccccga tgatatggtg aatgcaccat tcaaatcgcc atcaaaaacg
     3601 attggggcga tcatccgcgg attcaaatcc gcaacgacca aacggatcaa tcaattacga
     3661 aatacaccgc gtttaccggt atggcaacga aattattatg aacatattat tcgtaatgac
     3721 gcatcgtatc atcaaatcac cgaatacatt cgtttaaacc ccgcgcgttg ggaagaggat
     3781 agattattca accaattaaa ataaagattc atgggcgcat ggctatgcgc cccaacgaat
     3841 gcgccccaac gggaaacgag cccaacgggg aataaaaccg attggcgtac gggggtatcg
     3901 catccgccct aacggataaa caggaatccg atgaaaatca ccgaatccga catagaatta
     3961 ttggccatcg ggcatttgga aaaattggga taccattatc tctacggccc gcatatcgcc
     4021 agcgacgggg aatcgccgga aagggagaat tacgcgcagg tcgttctgac cggacgattg
     4081 caggccgcca tcgcgcgaat taataagaat ttgcccgcgc ccgtgcagga acaggcgctc
     4141 aaggaagttc tgcgcgtggc tacgcccgat ctcttgcaga ccaatgaaaa ttttcaccgt
     4201 tttctcaccg aaggcatcac gctcgacgtg cgacacgagg acggagaacg cggcaaaacc
     4261 gtctggctcg tggattttga aaacccgtcg aataatgaat ttgtggtctg caatcaattt
     4321 accgtcatcg aaaacaatca gaataaacgc ccggacattg tgctgtttgt aaacggtctt
     4381 ccgctggttg tttttgaatt aaaaaatccc acggatgaga aggccacggt tcagaaagcc
     4441 tttcagcaat tgcagaatta caagacggcg attccatcgc ttttttat
//
LOCUS       DAOQGF010000188         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_10722_length_4377_cov_5.958584, whole genome shotgun sequence.
ACCESSION   DAOQGF010000188 DAOQGF010000000
VERSION     DAOQGF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10722_length_4377_cov_5.958584"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1666
                     /locus_tag="PK937_15345"
     CDS             305..1666
                     /locus_tag="PK937_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33998.1"
                     /translation="MSSRLLGLVAVMCFVFLVGCEQNNKIQKKPGVDNLTREEAREYI
                     KELARQLAEGNSASDGLEKTSMKASLMKASTLQKIMTEEKIALTELSPDIPEEIMVEG
                     KPKTLVLEIFNEETLKASMTSSGTLQKKAQLPGYGVIEDDEVFWDSNLPTVTVPAYLS
                     YDENGTTSVVEEQWILPYASDIDSIDDVFNMAIDEKMSSLEYPMFVVTIETPPALQQD
                     PPVEQIQLSKMSAVQSFLVLKGIRLRVERDGWTHEEVELWYGKEGTRTYKYNNSSYTV
                     TTTIVRYGWNMNGHTRKDASGVSRYFSDVNKKHKWYADQGWDVRLMALDNIYPRTLVA
                     WEDDDESGRICRYYEPWTPNVALDFDPVDSTRVVGQQRSWEVWDLSDNSIKPHTPITT
                     HIGGPGHSYIDQDDRFGESGVIRITDANVQHLSPSGVTFHSSNASPSGLGDIYYRFAW
                     SSN"
     gene            1858..2814
                     /locus_tag="PK937_15350"
     CDS             1858..2814
                     /locus_tag="PK937_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH33999.1"
                     /translation="MKFLIINLLIGISCSSQSCFKEYVETDSLISTDYDFIAGILAPD
                     SVKNFKRSYKTEVFIGKMFPADRNSQYVIHGVNDPVLYQMLYNTRQHYRVYYVPDSNA
                     NVLLISHQDSVKLTYTGFGIYRDLNSEVKINSGTTYSISVYKGNNGSYTATTAVPHNI
                     EITEPSSDILWSKPELSGSTWLSNISIKTSYLVRPTYFVLYSKLGNFIPWNFYSTNGN
                     DLTQNIIFQATAYDTTKVDSVNCYLEMRGMDNNFALFNQPYNFSATDDFWKDWYLKFE
                     ETRITNRSSVNGPNSVVGFLGSYNAASKQFIAKALWDSVLNK"
     gene            3107..3544
                     /locus_tag="PK937_15355"
     CDS             3107..3544
                     /locus_tag="PK937_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34000.1"
                     /translation="MLSIKTGINFAFANSADDQYQLSIRIPLFSRYHITLGGGYRDVQ
                     PKTAGIIATLETDLYRTEKHRIKFDLRHSYVYRKGHRLFDDRFHKTSIGFGAESIVWA
                     RFGLGVECAPIGIYYGTRHDNPEKGTPSAWGAREVRTNVFIVL"
     gene            3605..4123
                     /locus_tag="PK937_15360"
     CDS             3605..4123
                     /locus_tag="PK937_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34001.1"
                     /translation="MFKLSFVFLLLSYSAFAQSSFSKNTIYSEIAGPGVIYSFNYERS
                     FNDQISIRAGSSFLWQEQTGDLYDGKVIRLWTFPLTVQRLFRYKSHALEIGIGVSIMY
                     IYIPNSDKVRVSSDAFPLPSHEGLNTRGSGVLGYRFQRPEGGMVFRLGVYPIFTRKNL
                     TYWPGLGLGYAF"
BASE COUNT         1393 a          765 c          854 g         1365 t
ORIGIN      
        1 ccaattccaa ttccaattcc aattccaatt ccaattccaa ttccaattcc aatgggattt
       61 gaataaaggt acaacatatt tagagttata tgtgaaaaag gaggtgagtc cattcgtcat
      121 ctatcttgta ttttcgaaat taacattttc aaaatcggaa caaaaaaata ggaagaacaa
      181 tattacctga ttcagggctg ggagcattga atttttaagt aatactgtct tcctatgcaa
      241 tagtatctct aatataaggt acatttgcaa ctttttgttc ctcaaacact gaggagtgga
      301 taaaatgagc agtcgtttgc tcggcttagt agccgtaatg tgttttgttt ttttggttgg
      361 ttgcgaacaa aataacaaaa ttcagaaaaa acccggagtg gataacctga cacgtgaaga
      421 agctcgagag tatatcaaag aattggctcg acaacttgct gagggaaact cggcatcgga
      481 tggtcttgag aaaacaagta tgaaagcttc gcttatgaaa gcaagtacac tacagaaaat
      541 catgactgaa gagaaaattg cactaacgga gctttcaccg gatataccgg aggaaattat
      601 ggtggaaggt aaacctaaga cactggtgtt ggaaattttt aatgaggaga cattaaaagc
      661 tagcatgaca tcgtccggta cattgcaaaa aaaagcccaa ctgcctggtt atggcgtcat
      721 agaggatgat gaggtttttt gggatagtaa cttgcccacc gtaaccgtac cagcttacct
      781 ttcctatgat gaaaatggca caacctccgt tgtcgaggaa caatggattc taccctatgc
      841 aagtgatatt gacagcattg atgatgtctt taatatggct atagacgaaa aaatgtcttc
      901 gcttgaatac cctatgtttg tcgtaaccat agaaactcct cccgcactac aacaagatcc
      961 cccagttgaa cagattcagc taagtaaaat gagcgctgtc caaagtttct tagttcttaa
     1021 aggaataagg ctacgtgtag aacgtgacgg atggactcat gaagaagttg aactctggta
     1081 tgggaaagaa ggtaccagaa cgtacaaata taataattct tcatatacag taactactac
     1141 aatagtacgt tatggctgga acatgaatgg acatacaaga aaagatgcaa gtggagtttc
     1201 tcgttatttt tcagatgtaa acaaaaaaca caagtggtat gcggaccaag gttgggacgt
     1261 tcgattgatg gcacttgaca atatctatcc tagaacttta gtagcatggg aagatgatga
     1321 tgagtcaggg agaatatgca gatactacga accttggact ccaaatgttg cactagattt
     1381 cgatcctgta gacagtacca gagtagttgg ccaacaaaga agttgggaag tttgggattt
     1441 aagcgataat agcattaaac ctcatacacc gataacaacc catattggag ggcctggaca
     1501 ctcttatatt gatcaggacg acaggtttgg agaatcaggg gtaatcagga ttacagatgc
     1561 aaatgttcag cacttatcac caagtggtgt tacttttcac tcaagcaatg cctcaccaag
     1621 tggcttgggg gatatatatt atagatttgc atggtcatca aattaataaa ttttctcata
     1681 tattcacgta tttgccgtgt acatggcaaa tacgtgaatt ctaataagaa taaaatcata
     1741 agttgtaaag ttgaatcaaa cacctaacga aaatatatta atttctcatc ttacttaatt
     1801 taataaagct ttttaagtat tattgttcat aacaacacaa aattcaatga agaaaatatg
     1861 aaatttttga tcatcaattt actcatcgga atctcatgct ccagtcaatc ctgcttcaaa
     1921 gaatatgttg aaacagactc gctaatttct actgattatg attttatagc tggtatactt
     1981 gcaccagata gcgtaaaaaa ttttaagcgt tcatacaaaa cagaagtatt tatagggaaa
     2041 atgtttccgg ccgatcgcaa tagccaatat gttatccatg gcgtaaatga tcctgtttta
     2101 taccaaatgt tgtataacac tagacaacat tatcgggtat actatgtacc tgattccaat
     2161 gcaaatgttc tattaatttc tcatcaagac agtgttaagt tgacatatac tggatttggg
     2221 atatacagag acttaaattc agaagttaaa atcaattctg gcactactta ctctatatcg
     2281 gtgtacaaag gaaacaatgg ttcatataca gctactactg cggttcctca taatattgaa
     2341 attaccgagc catcaagtga tatactatgg tctaaaccag agttaagcgg tagcacatgg
     2401 ttatcaaaca ttagtattaa aacatcatat ttggttagac caacttactt tgtactatat
     2461 agtaagctgg ggaatttcat accttggaat ttttattcta caaatggcaa tgacctaact
     2521 cagaatataa tatttcaagc gaccgcatat gatactacaa aagttgattc agtaaattgt
     2581 tatttagaaa tgagaggcat ggataacaat ttcgcactat ttaaccaacc gtataatttt
     2641 tcagcgactg atgatttttg gaaggattgg tatttaaaat ttgaagaaac acgcattact
     2701 aacagatcaa gtgtaaatgg ccctaatagc gttgtaggtt ttttggggtc ttacaatgca
     2761 gcatccaaac aattcattgc caaggcatta tgggatagtg tcttgaacaa atgataagaa
     2821 gtttgaaact taaataaaca aattggacat tattgatact agtcctttga agagatcaat
     2881 cttatttcta aagggtatat tttttattgt agggatttac tgataaatat aagtattgaa
     2941 tttgcagcat ttgagaataa gccaacattc aggatcaata tataaggtct atatggaaag
     3001 agttaataaa attgtatttt atttttcttt actgattctt caaattcctt tctttgcttt
     3061 ttctcagttt catttttttg aagttaaacc gagtaaatat tatgaaatgt taagcataaa
     3121 aaccgggatc aactttgctt ttgccaattc tgccgatgat caatatcaac ttagtattag
     3181 gattccctta ttttcacgat atcacataac gctgggcggc ggttatcgtg atgtgcagcc
     3241 taaaaccgca ggtattattg ctactcttga aacggacttg taccgtacag aaaaacaccg
     3301 tataaaattt gacctgcgcc acagttatgt ctatcgcaaa gggcaccgac tctttgatga
     3361 tcgttttcac aaaacgtcca ttggtttcgg cgcagaatcc atcgtttggg cgcgttttgg
     3421 attgggtgta gaatgtgcgc ctatcggaat ttattatggt acgcggcatg ataatcctga
     3481 aaaaggcacg ccttccgcgt ggggcgctcg cgaagtaaga accaatgttt ttatcgtttt
     3541 ataaatcgag ctttatttta ttcaaggtgt tagtagaata actagctcaa gaagctttat
     3601 acatatgttt aaactatctt tcgtttttct gcttttgtct tatagtgcat tcgctcaatc
     3661 atcgttttca aagaatacca tttacagcga aattgctggg ccaggcgtta tttactcctt
     3721 caattatgaa cgatcgttta atgaccagat cagcattcgt gccggttcct cgttcttgtg
     3781 gcaagaacaa acgggtgatc tttatgatgg taaggttatt agattgtgga catttccatt
     3841 gactgtacaa cgtctattta gatataagag ccatgcgtta gaaataggga tcggcgtttc
     3901 gatcatgtac atttatatcc caaattcgga taaagtgcgt gtatcgtccg acgcgtttcc
     3961 actgccgtcg cacgaagggc tgaatacgcg cggttcaggc gttttaggat atcgttttca
     4021 gcgaccggaa ggcggcatgg tctttcggtt gggtgtttac ccgatattta cacgtaaaaa
     4081 tttaacatac tggcctggcc ttggtcttgg ttatgcattc tgaatttgac aatattgaga
     4141 ggatacgcta gataacttat cgttcgcata atcaggtgat ttccatctaa aattgtagct
     4201 tcgtttcagt ttgtatgcat tcaattttat aagaacattt taacatctaa gtatcagagc
     4261 ttttaagttt taaaaaagca atttgtccgg tcgaaattct gcattcactc tctttgcatc
     4321 gcgatattcc cttgcgtaaa ctttctaagt tatgtatttt gcgctcgcat gaataac
//
LOCUS       DAOQGF010000189         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_11617_length_4158_cov_5.104801, whole genome shotgun sequence.
ACCESSION   DAOQGF010000189 DAOQGF010000000
VERSION     DAOQGF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11617_length_4158_cov_5.104801"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PK937_15365"
     CDS             <1..1134
                     /locus_tag="PK937_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34002.1"
                     /translation="ILPEMSTLVTSTADLTKPEVKEAAIQKIADFISAEPQMGMFASE
                     VVKEAKAIYDVTVKDYQTQIIEIPRITIQPKGEVKSGFKDFDLDVKNLNLRPSDQKIL
                     IHTLSNGNIEIIDADGVKVQYDSVDNILVNEVINFPEVDYDSSSALLFKLTGQAITKF
                     KSYLDEEDVINVIQNNKREIARFIYVQMMECFFYQAPDFEEPIITVKSFTRIEPHNYS
                     KMSGDKVYDLRDTIEPTHLIPSKIFTGFKKACHKEYKFDTKTEKDFAIMLESGGNNEV
                     VKWMRPAPNQFRIYYRHNSKQYRPDFVVELKDCIYLVETKKQQDVDDTDVQDKARAAL
                     EYCKYATEFTKKHGGKPWRYTLIPHTEVKFNATPATLMQRFLM"
     gene            1180..1689
                     /locus_tag="PK937_15370"
     CDS             1180..1689
                     /locus_tag="PK937_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HNH34003.1"
                     /translation="MLSVAEASNWEGVFFAQNLSINSKLLYYITVSPSKSVLKTCLKK
                     PTSIRQMVKTEIDKVDDTLKAWFKDKDFLIKPIDAKVQQCLKDIYAKDTRHLRLVDSS
                     KQRSLADPWVIAHAMAENAIVVTKENKITDSNSDKIKIPNVCESMGIAWMDDFQFVRT
                     MQISFDCKR"
     gene            complement(1697..1870)
                     /locus_tag="PK937_15375"
     CDS             complement(1697..1870)
                     /locus_tag="PK937_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34004.1"
                     /translation="MATNPPKGDHQRKGAVRDRSQTHNPKTDSWVKRDTKTGQFIDQK
                     ADDKPFKGVRKEK"
     gene            complement(1875..2222)
                     /locus_tag="PK937_15380"
     CDS             complement(1875..2222)
                     /locus_tag="PK937_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34005.1"
                     /translation="MMNYSAIPEFDRELKKLTKKYKSLPSDLELFKKVLAVAPEGNER
                     HFACLTKTDAFVVIKARLFSQYLKGSSLRIIYAYWKTNDCVEFIELYFKGDKETEDTA
                     RIKAYLKSKIQER"
     gene            complement(2209..2994)
                     /locus_tag="PK937_15385"
     CDS             complement(2209..2994)
                     /locus_tag="PK937_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HNH34006.1"
                     /translation="MNSLTKHIRYLRDSKSFSQEYVADIIGVSRPTYVQIEKGERELT
                     VSEVKKLAALYEMDISDLLMQETPTRKKITLDSKQPTKKAKEPSLRISVPQKNIEKFK
                     EVLLYILGKVGAKPNIGETAIYKLLYFIDFDYYEKYEEQLIGATYIHNHFGPTPVEFQ
                     AITDKMKVDNELTVVTNKYFQYDQKKYLPLRPAKLVVLNAQEKALIDDVLARLSDKSA
                     TELSEYSHGDIPWKIHAAGEVIEYDSVFYRDREYTVRNLDDEL"
     gene            3296..3859
                     /locus_tag="PK937_15390"
     CDS             3296..3859
                     /locus_tag="PK937_15390"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNH34007.1"
                     /translation="MNEGEDLSDIKVFRLLSQQSLSTAEAKLAADILYKEFIPKIIGV
                     LVKSGAERILAEDMAKTAVYKAIVCIKTYNPTKAKLITWVTKIAINLFFDKCRAGRNI
                     DTYSLNNSTIADEPVPDDEKGEPKQMEKLKDCVQKLSKDDQLLIQYELYRTKNEFLEE
                     FHITDDTYRTRKSRALQQLRLLVKDKG"
     gene            3911..>4158
                     /locus_tag="PK937_15395"
     CDS             3911..>4158
                     /locus_tag="PK937_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34008.1"
                     /translation="MNNKGDLRDIETLLDQMRKQQGVIPLTDDVVEFLLSQSEPIQPS
                     QKLLPELEVIISKAINERETSEQAQRQQASTFGEYIKA"
BASE COUNT         1290 a          874 c          838 g         1156 t
ORIGIN      
        1 atccttcctg agatgagtac ccttgtgacc agtacagccg atcttacaaa accggaagta
       61 aaagaggccg caattcaaaa gattgctgat ttcatatcgg ccgagcctca gatgggtatg
      121 tttgcgagcg aagtagtcaa agaagccaaa gcaatctatg atgtgacggt caaagactat
      181 caaacgcaga tcattgagat tccgcgtatt acgatacaac ccaaaggcga agtcaagtct
      241 ggcttcaaag acttcgatct cgatgtaaag aatctaaacc tcaggccatc cgatcagaaa
      301 atattgatac ataccctcag taatgggaac attgaaatca tagatgcgga cggcgtcaaa
      361 gtacaatacg attccgtgga taatatcctt gttaacgaag tcatcaattt tccagaggtg
      421 gattacgata gttcctccgc gttgctattc aagctgaccg gacaggcaat tactaaattc
      481 aaatcgtatc tggacgaaga agacgtgatc aacgtgatcc aaaataacaa acgagaaata
      541 gctcgtttta tctatgtaca gatgatggag tgcttcttct accaggctcc cgattttgaa
      601 gagccgatta tcacggtaaa atccttcacc cggatcgagc ctcataatta cagcaagatg
      661 tccggcgaca aagtctacga tttacgtgat acgatcgagc cgactcattt gatcccatct
      721 aagattttca caggcttcaa aaaagcctgc cacaaggaat ataaatttga cacgaagacg
      781 gaaaaagact ttgcaatcat gttagaaagc ggaggaaata atgaagtcgt gaaatggatg
      841 cggccggccc ccaatcaatt caggatttac tataggcaca attcaaaaca ataccgtccg
      901 gattttgtgg ttgaattgaa agattgtatc tacctggttg aaacaaagaa acagcaagat
      961 gtagacgata ccgacgttca ggataaagct cgcgccgcgt tagaatactg caaatacgcg
     1021 acggaattca ccaaaaaaca cggcggaaag ccctggcgtt atacattgat tccacataca
     1081 gaagttaaat ttaacgcgac gcccgccaca ctcatgcaac gattcttaat gtaaattagc
     1141 ttagaaaatg ccttcctctg ccacggaagg acaggggctg tgctgagcgt agccgaagca
     1201 tcgaactggg aaggtgtctt tttcgctcaa aatttgagca taaattcgaa gcttttgtat
     1261 tacattaccg tatcaccaag taagtcggtt ttaaaaacat gtctgaaaaa gcctacatca
     1321 atccggcaaa tggtcaaaac tgaaattgac aaggttgacg acacgcttaa ggcttggttc
     1381 aaagacaaag acttcttgat caaacctatc gatgcaaaag tccaacaatg cctaaaggac
     1441 atctacgcca aagatactag acatctgcgc ttagtagata gctccaaaca gcgttccctc
     1501 gccgatcctt gggttatcgc tcacgccatg gccgaaaacg ccatcgtagt gaccaaagag
     1561 aacaagatca ccgactccaa ttcagacaag atcaagatcc ccaacgtctg cgaaagtatg
     1621 ggtatcgcat ggatggatga cttccaattc gtacgcacaa tgcagatcag tttcgattgt
     1681 aagagataaa cttccgctat ttttcttttc tcacaccttt gaatggcttg tcgtcggcct
     1741 tttgatcaat gaattgacct gttttcgtgt ctcgttttac ccatgaatca gtttttgggt
     1801 tgtgtgtttg agagcgatca cgaacagcgc ctttgcgttg gtgatcacct ttagggggat
     1861 tggtcgccat gatcttacct ttcttgaatt ttggatttca aatatgcttt tatacgagct
     1921 gtatcttcgg tctctttgtc acctttgaaa tagagctcaa taaactcaac acagtcattg
     1981 gtcttccagt acgcgtagat aatgcgtaga ctgcttccct ttagatattg agaaaacagc
     2041 ctggctttga tcacaacaaa cgcatcggtc tttgtaagac atgcgaaatg acgctcattt
     2101 ccttctgggg caaccgccag aactttcttg aagagttcca aatcgctggg tagcgattta
     2161 tacttttttg ttagtttctt gagttctcga tcaaactcag gaattgcgct ataattcatc
     2221 atccagattt ctcacggtat attcccggtc acgatagaaa acactgtcat attcgatgac
     2281 ttcacccgca gcatggattt tccacggaat atcgccatga gaatattcgc tcagttctgt
     2341 agcgctctta tctgacagcc tcgccaatac atcatcgatt aatgcctttt cttgagcatt
     2401 taaaaccacc aattttgctg gccgaagcgg taaatatttt ttctgatcat attgaaagta
     2461 tttattcgtt accacggtaa gctcgttgtc aaccttcatc ttatctgtaa tagcttgaaa
     2521 ttccaccggg gttggaccaa aatggttgtg aatataagtc gctccgatca attgctcttc
     2581 atatttttca tagtaatcaa agtcaataaa atagagtagc ttatagatag cggtttcacc
     2641 gatattgggt ttggcgccga cctttcccaa gatataaagt aaaacttcct taaatttctc
     2701 aatgttcttc tgaggtacac ttatacgaag tgatggctct ttcgccttct tggtaggttg
     2761 tttgctatcg agggtaattt tttttctcgt gggtgtctcc tgcatgagga gatctgaaat
     2821 atccatttca tagagtgccg ccagtttttt tacttccgaa acagtcaatt ccctctcacc
     2881 tttttcaatc tgaacatagg taggccggga caccccaata atatccgcta catattcctg
     2941 cgagaaactt tttgagtctc gcaagtatct aatgtgtttt gtcaatgaat tcataggtac
     3001 tccggttttt ttctttgctt taaccattca atctgacgga cggcttcacc cacgatgact
     3061 taacgcagtg tacagggtat tatttcagca ttccagattg gtttagaaat ataggaataa
     3121 accaggtcga tgtcaagtta tataacatta ggaattgtaa agaaatgaaa ctgtgacaac
     3181 tcacttttcg taacatatta aaaatcaatc agttacaagt tgttattgtt ctctgtcaca
     3241 ggcacacgta taaataggta taaagtaatt aatcaaagta tggagaattc cgtgaatgaa
     3301 tgaaggcgag gatttgtctg atataaaagt ctttaggcta ctgtctcagc aatcgctatc
     3361 aactgctgaa gcaaaacttg ctgccgacat tttatataaa gaattcatac caaaaatcat
     3421 cggcgtactc gtaaagtccg gtgccgaacg tattcttgct gaagatatgg caaaaacagc
     3481 cgtttataaa gccatcgtgt gcataaagac atataacccc acaaaggcca aacttatcac
     3541 ttgggttaca aagattgcca tcaacttatt ctttgataaa tgtagagctg gacgaaacat
     3601 tgatacgtat tccctaaaca atagtactat tgccgatgag cccgtcccag atgatgaaaa
     3661 gggtgaaccc aagcaaatgg aaaaactaaa ggactgcgtt caaaagttat ccaaagacga
     3721 ccagttgtta attcagtatg aactgtatcg cacaaaaaat gaatttctcg aagaatttca
     3781 tatcaccgac gatacgtaca gaacaagaaa atctagggcc ttgcagcagt tacgtctctt
     3841 agttaaagat aaaggatagg gggttgacaa aacaattaag aataccaaac acgttatatg
     3901 gaggaacgtt atgaataaca aaggtgatct aagagatata gaaaccttgt tagatcagat
     3961 gagaaagcaa caaggagtga tcccccttac tgatgatgta gttgagtttt tgttgtctca
     4021 atctgaaccg attcaaccct ctcaaaaatt actgccggag cttgaagtca taatatcgaa
     4081 agccattaat gagcgggaaa cctcagaaca agctcaaaga cagcaggcgt cgacgtttgg
     4141 agagtatata aaggcatt
//
LOCUS       DAOQGF010000190         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_11675_length_4145_cov_9.154279, whole genome shotgun sequence.
ACCESSION   DAOQGF010000190 DAOQGF010000000
VERSION     DAOQGF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11675_length_4145_cov_9.154279"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1065
                     /locus_tag="PK937_15400"
     CDS             178..1065
                     /locus_tag="PK937_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HNH34009.1"
                     /translation="MSNPLAEVFGFPTNNKSAEAKRYRRLRLCPYNNKVPSCTKDKAQ
                     NPLGVCTIHDGNELAITCPIRFREDWIIAENASEFFFGKKVNWTSLTEIRLNDKYGKS
                     AGNIDLVLVSYDVSGKITDFGSLEIQGVYISGNVRRPFDKFMKNSENASDFDWSDEPN
                     YPHADYLSSSRKRLVPQLLYKGGILNSWKKKMAVALHEGFFKTLPELPTVPKEKADVA
                     WFIYSMRYDQKQKRYRLVLKDTVYTKFQDALNKISVSEPGDMSEFIELLQSKLDEKLE
                     NTIPPDAPILTDIITPEND"
     gene            1067..1954
                     /locus_tag="PK937_15405"
     CDS             1067..1954
                     /locus_tag="PK937_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNH34010.1"
                     /translation="MSQLDLYRETEHSTEQVVNVASVRQLSPFRYPGGKTWLVPQAMR
                     WVKKRRNGNCKVFVEPFVGGGIISLTIAAHGLADKCILVERDDEIAAVWKTLIYGDAE
                     WLARRIRNFELSADTANEVLSSEPKSTEEKAFQTILKNRTYHGGILAPGSGFLKNGEN
                     GKGIHSRWYPETLSNRIQAILQFKHRLEFIEGDAFEIIDQYIDDSSCTFFIDPPYTAS
                     KKKAGSRLYRFHEVDHEKIFEKMNQTAAAFMLTYDDAPEIRDFCDKYSLDAVRVPMKG
                     THHIKYYELLICDNVTWIQ"
     gene            2197..2808
                     /locus_tag="PK937_15410"
     CDS             2197..2808
                     /locus_tag="PK937_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH34011.1"
                     /translation="MMFRQKCIPVLAVLVCSCAGTNPNPGERTADVLWSKYRHEESLK
                     ISRPAAERGEPWAQLRLGIAYELGLGVPKDIPKALYWYKLTSVHKGSDKWSEGLLIGA
                     IGQAGYFNQNSDAMVAEYQIANIYLNGDGVEKDLVAAFLWIKYLEKESAGKSVFICCE
                     FEGGRWIPQKEIQRTIDDIEKEIDPEQRKKAEQLLVTWKPSLE"
     gene            3074..3487
                     /locus_tag="PK937_15415"
     CDS             3074..3487
                     /locus_tag="PK937_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34012.1"
                     /translation="MKPILFVIMSLGIINCSEKKESYPECMEHSWKITLIGKDTIVGT
                     MELYNYSNGFPFAGESMDATWSVLNKYTYSHIGTGYINNWTALFYINDEDNVYNYQFS
                     FSRFDCDDFFGTCLLIRNIDSVIDSSDYNFKAKRM"
BASE COUNT         1280 a          681 c          934 g         1250 t
ORIGIN      
        1 gttactcgca gcatgccgat actgtggcgc agggaactaa tagcaaaagt tttcagatac
       61 cctgcgccac agcaccgcag atagtcgcga accgttatgc gagatcccag cggctgtttt
      121 tgtttgatta accggtttct ttttgtaatt tggatcaaat aaacatagaa agttcagatg
      181 tctaatccat tagcagaagt ttttggtttt ccaacaaata ataagtcagc tgaagcaaag
      241 agatataggc ggctgcggct atgtccttat aataacaaag ttccttcttg cacaaaagat
      301 aaagctcaaa atcctcttgg ggtttgtacg attcatgatg gaaatgagtt agctattact
      361 tgccccataa gatttcgaga ggattggatt atagctgaaa atgcctccga attctttttt
      421 ggaaagaaag tgaactggac ttctttgacg gaaattcgtc taaacgataa gtatgggaag
      481 tcggctggaa atattgattt ggtacttgta tcgtacgatg tgagtggaaa aataactgat
      541 tttggctcgc tcgaaatcca aggtgtttac atttcaggta atgtacgtag accatttgat
      601 aagtttatga agaattctga aaacgcatca gatttcgatt ggtcagatga accaaattat
      661 ccgcatgcag attacctctc atcatcgcga aaacgacttg tgccgcaatt attatataaa
      721 ggcggtattc taaatagttg gaagaagaaa atggctgttg ctttgcatga agggtttttt
      781 aaaacacttc cagaattgcc aacagttcct aaagaaaaag ctgatgttgc ttggtttatt
      841 tattcaatga gatacgatca aaagcaaaaa cgatatagac tagtacttaa ggatactgtt
      901 tataccaagt ttcaagatgc gttaaataag atttcagtat ccgaaccggg tgatatgtca
      961 gaattcatag aactattaca aagcaagctt gatgagaaac tagaaaatac tataccacca
     1021 gatgctccaa tattgactga tataattact ccagaaaacg attgatatgt cccaattaga
     1081 tttatataga gaaacagaac attcaactga acaggttgta aatgttgcct ctgtaagaca
     1141 gttgagccct tttcgttatc cgggaggaaa aacttggcta gttcctcaag ctatgcgttg
     1201 ggtaaaaaag cggcgaaatg gtaattgcaa agtatttgta gaaccttttg ttggaggcgg
     1261 aataattagt ttaacaattg cggcacacgg gttggcggat aaatgtatcc ttgttgagcg
     1321 cgatgatgaa attgcagcag tttggaaaac gttgatctat ggtgatgcgg aatggttggc
     1381 tcgtaggatt agaaattttg aattaagtgc ggatactgca aatgaagttt tatcgagcga
     1441 gccaaaatct actgaagaga aagctttcca aacaatattg aagaatcgaa catatcatgg
     1501 aggtatactg gctccaggtt ctggtttttt gaaaaatggt gaaaatggta agggaataca
     1561 ttctagatgg tatccagaaa ccctctccaa tcgtattcaa gcaatattac agttcaaaca
     1621 taggcttgaa tttatcgaag gcgacgcttt tgagataatt gatcaatata ttgatgatag
     1681 ttcttgtaca ttttttattg atcctccata tacagcatca aaaaagaaag cgggttctag
     1741 gttgtataga tttcatgagg ttgatcatga aaaaattttt gaaaaaatga accaaaccgc
     1801 agccgctttt atgcttactt atgacgatgc tccggaaatc agagattttt gcgataaata
     1861 ttcattggat gctgtcaggg ttcctatgaa gggcactcat cacatcaaat attatgaact
     1921 attgatttgt gataatgtta catggataca atagccgctg ggacatctgc ataaccatcc
     1981 ggtaatccgt tcgcacgatg ctcgtaaaga gttgaattta gcttttctat aagtaattaa
     2041 taaataccta acaatagtaa tttaaactcg catcgtgccg atactgtggc gcgaggaacg
     2101 agtagattta agttttcaga tacctcgcgc cacagcaccg cggataccgg agaccgttat
     2161 gagatatgcc gctctaccac ttgttaacaa ggagtgatga tgtttcgaca aaaatgtatc
     2221 ccggtcctcg ccgttttagt gtgtagctgt gccgggacga atccaaatcc cggcgagagg
     2281 actgctgacg ttctttggtc aaagtatagg cacgaggagt cgctaaaaat tagcagacca
     2341 gctgcagaaa gaggcgaacc ttgggctcag ttgcgtttag gcatcgcgta tgagcttggt
     2401 ctcggcgtac caaaagacat tccaaaagcg ttgtattggt acaaactcac ttccgttcat
     2461 aaagggagtg ataagtggtc ggaaggactg ttgattggag caatcgggca ggccggatat
     2521 tttaatcaaa acagcgacgc aatggttgct gagtaccaaa tagcgaatat ttatttgaat
     2581 ggggatgggg tagagaaaga tttggttgca gcattcttgt ggataaaata tttggaaaaa
     2641 gagagtgcgg gaaagagtgt gttcatttgt tgtgaatttg aaggtggccg atggattccc
     2701 cagaaggaga tccaaaggac tattgatgat atagaaaagg aaatcgatcc ggaacaaaga
     2761 aagaaggccg aacaattgct agtaacgtgg aaaccatctc tggaatgagt gccattttac
     2821 ccaaatcaat taagctgtga gcggcacatc tcataacatc gccacaggcg ttcgctcact
     2881 gctcgcagag tattgaaaat agggtttttg tagatgaatg tatcattttt aaataataaa
     2941 ttaggctcgc agttcgcata tatcgtggcg cgaggaacga gtagatttaa gttttcagat
     3001 acctcgcgcc acgacacatc gcctatggcg tgccgttagg cgacatccgt tatggacttt
     3061 cggagtagta tttgtgaaac caattttatt tgtaataatg agtttaggga taattaattg
     3121 ttctgaaaaa aaggaaagtt atcccgaatg catggaacat tcgtggaaaa taactttaat
     3181 tggtaaagat actattgttg gaactatgga gctgtataat tatagcaatg gatttccgtt
     3241 tgctggagaa agtatggatg caacatggag tgttttaaat aagtatacat attctcatat
     3301 tggtacaggt tatatcaata actggaccgc cttgttttat ataaacgatg aagataatgt
     3361 atataattat cagttttcct tttctaggtt tgattgtgat gatttttttg gaacttgttt
     3421 gttaattcgt aatatagaca gtgttattga cagttcggat tataatttta aggctaaaag
     3481 aatgtaaaat aacggacgtc gcctaaccat ccggtgatcc gttcgcacga tgctcgtaga
     3541 gtattggaag tagggttttt ataagtaatg aatagattta taacaaataa ttgtaactcg
     3601 catcgtgccg atactgtggc gcgaggcacg agagatatga agttttcaga tacctcgcgc
     3661 cacagcaccg cggataccgg agaccgttat gcgctatgca ctttgatcgt tttgtgatgg
     3721 atttaaaatc ataacgaatt gttctttgaa aagtaacggc ggcgtatgaa aatcattttg
     3781 tagtttcttt atttgaagtt cattttgttc gtgtttccac tttttttttg aatctcaatt
     3841 gatagtagtt ttttttgaaa gttgattgat ctgaatttca agtgacacga acaaaatcta
     3901 aaggttcatt gaaaagttgg tgatttagag ttttaaggtg ttagattggg ataatctcaa
     3961 acaatacgcc gccttgaact tatgggaatg ttattcgttc tgattttgat ttgatcatag
     4021 aggtgttctt ttcagaaaag tatagtaatt cgtgcacagc gcataacagc agcacagacg
     4081 ttcgttcagc agccttgaag ttcattgaat agttggtagt attttgctcg tcatccccgc
     4141 ttgag
//
LOCUS       DAOQGF010000191         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_11905_length_4096_cov_6.858698, whole genome shotgun sequence.
ACCESSION   DAOQGF010000191 DAOQGF010000000
VERSION     DAOQGF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11905_length_4096_cov_6.858698"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..516)
                     /locus_tag="PK937_15420"
     CDS             complement(133..516)
                     /locus_tag="PK937_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH34013.1"
                     /translation="MPTWMLLLVTGVILWSLYKKKLGKNTMFQNLNNDAFSEKMKNAD
                     HVVLDVRTDAEYASGHLPKAKLINVMDASFSSKVDALDKSKTYLVYCRSGSRSRGACE
                     IMASRGFQVFNLDNGIMGWNGPVER"
     gene            complement(528..1931)
                     /locus_tag="PK937_15425"
     CDS             complement(528..1931)
                     /locus_tag="PK937_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNH34014.1"
                     /translation="MYFQNIYEKGLAQASYLIGCQATGTAIVIDPRRDIHVYLEAAAR
                     EKFTITHVTETHIHADFLSGARELAAATGAELLLSDEGGADWQYAFPHTGLRDGSVFK
                     VGNLKFEVMHTPGHTPEHIAFVLTDVPAGDYPIMFFSGDFVFVGDIGRPDLLEKAAGV
                     TGTMERGAQQMFHSLKKFKSLPDHVQVWPGHGAGSACGKALGTVPGTTVGYEKLVNWA
                     LRYTDENEFVKELLSGQPEPPYYFAMMKKLNKEGPPILGTSSGLKRLTTDDATQAIRN
                     GQTLIDTRPKTAFAEKHIPGAINIQNNNALSTWAGWILSYDKPFLILVSENDAQDVYH
                     KLIRIGLDHCTGYITENRFWEESGGTLHSLRQIFATDLSAKLSDPNVAVIDVRGVSEY
                     ETGHIAGAVHIHGGHLSRRLADLPKNKTLIIQCQGGDRSSTATSLLLKNGFSNIYNLI
                     GGIAAWQKAGLPVVKEN"
     gene            2073..2414
                     /locus_tag="PK937_15430"
     CDS             2073..2414
                     /locus_tag="PK937_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNH34015.1"
                     /translation="MAKKLFDKEKMSEESLALIAKRFKALSEPMRLKIIQTIWHSEMS
                     VQDIVAKVQTSQVNVSKHLAVLHECGFLKRRKEGLFTFYSVADTSVFTLCDMMCSVVK
                     DKLNDQIRSFS"
     gene            2414..2794
                     /locus_tag="PK937_15435"
     CDS             2414..2794
                     /locus_tag="PK937_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH34016.1"
                     /translation="MMYRVGIIIFMLATACGAQDSFRSVNSDTLNQLLKDKPGVLLDV
                     RTVGEFSTGHLKDAVLLDFMKPEFESELEKLDKSATYYVYCRSGNRSAKAASLMNQKG
                     FQSVYNVADGMIGIVKTMPDQVVR"
     gene            2941..3381
                     /locus_tag="PK937_15440"
     CDS             2941..3381
                     /locus_tag="PK937_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNH34017.1"
                     /translation="MAGYSGTPLVKKIGIKEGYSVAWVDAPATLKALIEPLPEKARLT
                     RKVVADHDCVHWFTKSADSLEIELPKLKKKIKDSGMIWVSWPKKSSGVRTDITEDMIR
                     ECARRNGLVDVKVCAVDDVWSGLKLVIPVKDRTSLTKKNKSPKL"
     gene            3402..>4096
                     /locus_tag="PK937_15445"
     CDS             3402..>4096
                     /locus_tag="PK937_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNH34018.1"
                     /translation="MKLVLRIVAGLFGLFILAIIGGYVYIKTALPNAGPVPQMVIASS
                     SEQIERGKYLANHVAVCTDCHSKRDYSFFAGPLKPETFGAGGEEFPESMGFPGTLYAR
                     NITPAGLKDWTDGEIFHALTTGIRKNREPIFPLMPWHRYATSDKEDILSIIAYIKTLK
                     PIENQVPETRLNFPLNLIVRTMPKAPAFVTRPDTTDEVAYGKYLANMAACADCHTMTK
                     KGEPLPGMDFAGG"
BASE COUNT         1118 a          909 c          953 g         1116 t
ORIGIN      
        1 agattgaccc acaagctgga ttgcagcggg cacgcaccgg aaagacaacc gatgtatttc
       61 caataaaaga atcccgcgat aattcctgcc aacggcccga tccaaaaacg atgttttttt
      121 atatattcca tattaacgtt cgaccggccc gttccaaccc atgatgccat tgtctaaatt
      181 gaaaacctga aacccgcggc tggccatgat ttcacaagcg ccgcgtgaac gactgccact
      241 acggcaatat acgaggtatg ttttagattt atccaaagca tccactttgc tgctgaaact
      301 tgcatccatt acgttgataa gtttggcttt gggcaagtgg cctgaggcgt attcggcatc
      361 ggtgcgtaca tccaagacca catgatcagc atttttcatt ttttcactaa atgcgtcatt
      421 attcagattt tgaaacatag tattttttcc taattttttt ttatataaag accataaaat
      481 tacgcctgta actaacaaaa gcatccacgt cggcattttc tgctccttta attttctttg
      541 acaaccggca aaccggcttt ttgccatgcg gctattccgc cgatcaggtt ataaatatta
      601 ctaaatccat ttttgagcaa aaggctggta gcggtactgg agcggtcccc gccctggcat
      661 tgaataatca gcgttttgtt ttttggaaga tcggcaagcc gtcgcgacaa atgaccgcca
      721 tgaatatgta cggcccctgc gatatgaccg gtctcgtatt ccgatacgcc acgaacatca
      781 atgacggcaa cattcgggtc cgataatttc gcagataaat ccgtagcaaa aatttgccgg
      841 agactgtgga gcgtgccgcc gctttcttcc cagaaacgat tttcggtgat atagccggtg
      901 cagtgatcaa gaccaatgcg aattaattta tgatatacat cctgcgcatc attttccgac
      961 acaagaatca gaaacggttt atcgtaagac aatatccatc cggcccatgt tgacaaggca
     1021 ttgttgtttt gaatgttgat agcgccgggt atatgttttt cagcaaatgc ggttttgggg
     1081 cgcgtgtcga tcagagtttg accgttgcgt atagcctgcg ttgcgtcgtc agttgtcagg
     1141 cgttttaacc cggacgaagt gcctaaaatc ggagggcctt ccttattcag ttttttcatc
     1201 atcgcaaaat aatatggcgg ttccggttgt ccgctaagga gttctttgac aaattcattt
     1261 tcgtcggtat aacgcaacgc ccaattgacc agtttttcgt aaccgaccgt agtacccgga
     1321 actgtgccca gtgctttacc acaggccgaa ccggcgccgt gaccgggcca cacctgaacg
     1381 tggtcgggaa gggatttgaa tttttttaac gaatgaaaca tttgttgcgc accgcgttcc
     1441 atcgtacccg taacacccgc ggctttttct agtaagtccg gacgaccgat gtcgcctaca
     1501 aaaacaaaat cgcctgaaaa aaacataatc gggtagtcgc cggccggcac gtccgtgagt
     1561 acgaaggcga tatgctccgg cgtatgtccc ggcgtatgca tgacttcaaa tttcagattg
     1621 cctactttaa aaaccgaacc gtcgcgaaga ccggtatgcg gaaaagcata ttgccaatct
     1681 gcaccgcctt cgtccgacaa taacaactca gcgcctgtcg cagccgccaa ttcgcgcgcg
     1741 ccactcagaa aatcagcatg gatgtgtgtt tcggtgacgt gcgtgattgt aaatttttca
     1801 cgcgcagcag cttccagata aacatggata tcccggcgcg gatcaatgac gatcgctgtc
     1861 cctgttgcct gacagccgat gagataactg gcctgtgcga gccctttttc ataaatattc
     1921 tggaagtaca tgcttcactc caaaccgatt gacttttagt tggaatatcg ttatataact
     1981 acatagtagt atagagatat tttaatagga agtcaagtat tttggcattt tgactttatt
     2041 ttgagtaacc attttacgaa gttaaccaac ctatggcaaa aaaattattt gataaagaaa
     2101 aaatgagcga agaatcgctg gctttgatag ccaagcgttt caaagcgcta tcagagccca
     2161 tgcggcttaa aatcatacaa acgatttggc acagcgaaat gagcgttcag gatattgtgg
     2221 caaaagtgca gaccagccaa gtcaatgttt ccaaacatct cgccgttttg catgaatgcg
     2281 gctttctcaa acgcaggaaa gaaggattgt tcacatttta cagcgtggcc gatacctccg
     2341 tatttacgtt gtgtgatatg atgtgctctg tcgtcaaaga caaactcaac gatcaaatca
     2401 ggagtttttc ataatgatgt atcgcgtcgg tattatcatt ttcatgctag caacagcgtg
     2461 cggagcgcag gatagttttc gttctgtcaa ttcggatacg ctcaaccaat tgctaaaaga
     2521 caaaccgggc gttttgctgg atgtgcgcac ggtcggtgaa ttttcaaccg gtcacttgaa
     2581 ggatgccgtg ttattggatt ttatgaagcc ggaatttgaa tccgaattgg aaaaattgga
     2641 taaatctgcg acgtattatg tgtattgccg gagcggaaac cgcagcgcta aagcggcttc
     2701 gcttatgaat caaaaaggat ttcaatcggt atacaatgtc gccgacggaa tgatcggtat
     2761 tgtgaaaacg atgccggatc aggttgttcg ttgaatccgg gtataaaaat ttaggctgca
     2821 cagttctcaa aaaagataat tttggtgtat ttttttcgaa ttaactttgg cttcgtgtaa
     2881 cacgtcagac aagaccgttt tttattttaa aaattatctc ttaacacgga gaaattttct
     2941 ttggccggat attcaggtac gcctttagta aaaaaaatag gtatcaaaga aggctattca
     3001 gttgcctggg ttgatgctcc tgcgacattg aaggcgttaa tcgaaccgct tccggaaaaa
     3061 gctcgcctga cacgtaaggt cgtagcggat catgattgcg tccattggtt taccaagtcg
     3121 gccgatagtt tggagattga acttccaaag cttaagaaaa agatcaaaga ttcggggatg
     3181 atatgggttt cttggcctaa aaaatcatct ggtgttcgta ccgatattac ggaggatatg
     3241 attcgtgaat gtgcacgccg caacggattg gtcgatgtca aagtgtgcgc ggtggacgat
     3301 gtgtggtccg gtctgaaatt agtcattccg gtgaaagaca gaacatcgct tacgaaaaaa
     3361 aacaagtcac ctaaattgta aacccatttt cggagaaagc catgaaactt gtattgcgta
     3421 ttgtcgccgg tttgtttggg ctatttattt tggcaatcat tggcggatat gtatatatca
     3481 aaactgcgct gccgaatgca ggtcccgtgc cgcagatggt gatcgcatcg tcatcggagc
     3541 aaattgaacg cggaaaatat ttagccaatc acgtagcggt atgtacggat tgtcattcga
     3601 agcgtgatta ttcatttttc gcaggcccgc tcaaaccgga aacatttggc gccggcggcg
     3661 aagaatttcc cgaatctatg ggttttcccg gtacattgta cgcacgtaat attacgccgg
     3721 ccggtttaaa agattggacg gacggagaaa tctttcatgc actcacgaca ggtattcgga
     3781 aaaacagaga accgattttt ccgttaatgc cgtggcatcg ttatgcgacg agcgataaag
     3841 aagatattct gtcgatcatt gcgtacatca aaacattgaa gccgattgaa aatcaagtgc
     3901 cggaaacgcg gctcaacttc cctctcaacc tgatcgttcg caccatgccc aaagcacctg
     3961 cattcgtgac gcgtccggac acgacagatg aagtcgcata cggtaagtat ttggccaata
     4021 tggcagcctg cgcggattgt catacgatga ccaaaaaagg tgaaccgttg cccggaatgg
     4081 atttcgcggg cggatt
//
LOCUS       DAOQGF010000192         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_11918_length_4093_cov_5.482912, whole genome shotgun sequence.
ACCESSION   DAOQGF010000192 DAOQGF010000000
VERSION     DAOQGF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11918_length_4093_cov_5.482912"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="PK937_15450"
     CDS             complement(<1..1629)
                     /locus_tag="PK937_15450"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNH34019.1"
                     /translation="MRKQLLTLLFFLVSGVELVVAQERIFEKPLSPRIANYDLRAELK
                     PELKTVEASWNLHWWNASRDTVKELQFHLYLNAFRNERSTFIRESGGRHRSGEINLDS
                     ANWGYILIKNLAMAGGENLTTRIEYIAPDDGNKDDRTVIRVPLSRPIPPGGDIRLTCD
                     FTSKLPRVYARTGFTEDDFYLIGQWFPKIGVWENGAWNCHQFHSNSEFYADFGVYTME
                     LTVPKKYVVGANAVLMEKKALNDSTNLFRYRQEDVIDAVWTAYPHFKELQQTVTIKET
                     GRQVVVTYLVPPERLWVMPKYYATSEAMFNYYQEWYGAYPYPNLTIVDMPVGEEMTAG
                     GMEYPTLVTTGSYFGWPFINKYIVPNHWLETVTFHEFGHNYFQSLVATNEFEHPWMDE
                     GFTQYTEARASVRFAKENNIAYEMADIFGIPFNVLAYNRSSYTSRPKDGVIEAFSWNI
                     PESFYQPAAYAKPALVLETLQRHLGDAMWNKVMRTYFERYSFKHPKPRDFYAVVNEVT
                     GKDFMWYFDQFVNKPVTVDYQIAGIFNEEIHVSRG"
     gene            1795..1877
                     /locus_tag="PK937_15455"
     tRNA            1795..1877
                     /locus_tag="PK937_15455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1828..1830,aa:Leu,seq:cag)
     gene            complement(1912..>2748)
                     /locus_tag="PK937_15460"
     CDS             complement(1912..>2748)
                     /locus_tag="PK937_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34020.1"
                     /translation="KDRNIPRRFDHPVWFMTMRKIGFQAENKTKKNDALYKDYSINEL
                     QGEEKVAVSSTLDMKLTRWTRDLIIENYIKSEYGRIKLGNDPYRESDDNWSVESLVAL
                     PLIEQPIVSSVAVYPYAASKFQSEFTADQGPRKKDLYIHTGAAFSDFWNFSENRVGLV
                     HLTRIEGNERRIGNFGVGVHSLFSNTRGNYGNFSEFEFIYFFTKAKKKKSVAEADSAS
                     SNTRSLRFNWSSLIAIERFKNFYFNPGVNFFAYKGYGRDKWAYSVALQFGLVFTAGWK
                     FW"
     assembly_gap    2749..2848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2849..>4093)
                     /locus_tag="PK937_15465"
     CDS             complement(<2849..>4093)
                     /locus_tag="PK937_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34021.1"
                     /translation="FFCICLLLNVAHHPAVTQSLHIVATGDTKGYLFEENVEFGKSGG
                     ILERQAAITQLQSAYGRQNMLLFDTGDALGYYYLTTASHGRIVLDKMRDIGYDAMVVG
                     NHEFDFGKDVLKAYVDDPSYPSILGANVLGADSLPYLQPYKIVEKAGLRVGIIGVTDP
                     AITQSVLEEKMAGIIIADPSAAIRAYIDEVREQSDAIVLLTHLSEEENNDFAREFPEI
                     NVIIGKSSYIAPDRFSSVRVSDRFETFVFSVDKFGRNIPHITMDVQDRKIHDVQSVEV
                     IELASETIYVDPAVQAAFEEEVELAYAEHCETAYGLGPSSSILKKNWMGNKDLRDYMM
                     AMMLFKANAEIAVINDRFFDFDNYIANAVQTDTLTVRDFERIFWIENDIVTLRIKGED
                     ILKIIKRSDESRTKYNDRFVHL"
BASE COUNT         1092 a          999 c          835 g         1067 t
ORIGIN      
        1 gccccgggac acatgtattt cttcattaaa tatccctgct atctgataat ctacggttac
       61 cggtttattg acaaactgat caaaatacca cataaaatcc ttcccggtca cttcattgac
      121 caccgcataa aaatcgcgcg gtttgggatg cttgaaactg taacgctcaa aatacgtgcg
      181 catgacttta ttccacatcg catcaccgag gtggcgttgc agtgtttcca aaacaagcgc
      241 cggtttggca taggccgcgg gctgataaaa agattccgga atattccacg aaaaagcttc
      301 gatcacaccg tctttgggtc ggctcgtata gctgctccga ttataggcca atacgttgaa
      361 aggaataccg aaaatatccg ccatttcgta agcaatatta ttctcctttg caaaacgaac
      421 ggatgcacgc gcttcggtat attgcgtaaa tccttcatcc atccacggat gttcaaattc
      481 attggtcgcc acgagacttt gaaaataatt atgcccgaat tcatgaaacg tcacggtctc
      541 cagccaatga ttgggcacta tatatttatt gataaaaggc catccgaaat aagaacccgt
      601 cgtgacaagt gtcggatact ccataccgcc ggcggtcatt tcttcaccta caggcatatc
      661 tacgatcgtc agattgggat agggatacgc gccgtaccac tcctgataat aattgaacat
      721 cgcttcggaa gtcgcataat atttgggcat tacccaaaga cgctccggag gaactaaata
      781 cgtcacgacg acctgccgcc ctgtttcttt gatcgtcaca gtttgctgaa gttccttaaa
      841 gtgaggatac gctgtccaga cggcatcaat tacatcttcc tgacgatagc ggaatagatt
      901 ggtactgtcg ttgagcgctt tcttttccat aagcacggca ttggcgccga cgacgtattt
      961 tttaggaacg gttagctcca tcgtataaac gccgaagtcc gcgtagaatt cagaattgga
     1021 atgaaactga tggcaattcc acgctccgtt ttcccatact ccgatttttg gaaaccattg
     1081 accgatgaga taaaaatcat cttccgtaaa acccgtacgt gcatacacgc gcggcagttt
     1141 ggatgtgaaa tcgcaggtaa gccgaatatc tccgcccgga ggtatcggac gacttagcgg
     1201 aacacgtatt accgtacgat cgtccttatt tccgtcatcc ggtgcgatgt attcgatacg
     1261 cgtcgtgagg ttttctccgc cggccatcgc caagtttttt atgaggatat aaccccaatt
     1321 ggccgaatcc agattaatct caccggaacg atgccgtcct ccgctttcac gaataaaagt
     1381 agatctttca ttgcgaaatg cattgaggta aaggtgaaat tgcaattcct ttaccgtatc
     1441 acgactggca ttccaccaat gcagattcca cgatgcctcg acggttttca gttccggctt
     1501 caattccgcc cgtaaatcat agttggcaat acgcgggctc aaaggttttt caaaaatccg
     1561 ttcctgggcc acgaccaatt cgactcctga caccagaaag aaaagtaaag ttagtaactg
     1621 ttttctcatc tcccaattcc tcgatggatt taaaaagtac attagtaaac aaatttagaa
     1681 ctagaaacat cactagtccg ttagaccgct atacggtcaa aatgttgcgt tgttcaagct
     1741 ctatctaaaa tttatttgac gtttcaaata aattatctta ttattgcccg ccttgccgag
     1801 gtggtgaaat ggcagacacg ctagtttcag gtactagtgc ccgcaagggc gtgggggttc
     1861 aactcccccc ttcggcaccc aatcccgcaa acccattgcg ggattttttt attaccaaaa
     1921 tttccaacct gccgtaaata cgagaccaaa ttgcaacgcc accgaatacg cccatttgtc
     1981 tcgcccgtat cctttataag caaaaaaatt cacgccggga ttaaaataaa aattcttaaa
     2041 ccgctctatg gcgataagag atgaccagtt gaaccgcaaa gaacgcgtat tggatgacgc
     2101 gctgtcggct tcggccacac tttttttctt tttggctttg gtaaaaaaat agatgaattc
     2161 aaattcagaa aaattaccat agttcccgcg tgtattggaa aatagcgaat gcacaccgac
     2221 accaaaattg ccgattcgtc gttcattacc ctcgatccgg gttagatgga cgagcccgac
     2281 gcggttttcg gaaaaattcc aaaaatccga aaacgccgcc ccggtatgaa tgtataaatc
     2341 tttttttcgc gggccctgat cggccgtaaa ttcggattga aatttagacg ccgcataagg
     2401 atatacggct accgaactta cgatgggctg ttcgatgagc ggcaaagcca cgagactttc
     2461 gacggaccag ttatcatccg attctcggta aggatcatta cccaacttga tgcggccgta
     2521 ctccgattta atatagtttt ctataatgag gtcgcgcgtc cagcgggtca gtttcatatc
     2581 aagcgtggag gaaaccgcta ctttttcttc gccttgcaat tcgttgatgg agtagtcttt
     2641 atacagagcg tcattctttt ttgttttatt ctccgcttga aaaccgatct tgcgcatcgt
     2701 cataaaccac accggatgat caaaccttcg tggtatattc cggtctttnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gagatgaaca aaacgatcgt tgtatttggt
     2881 gcggctctca tcactccgtt tgatgatttt gagaatatcc tcgcctttga tgcggagtgt
     2941 gacaatatcg ttttcgatcc aaaatatacg ctcaaaatcc cgtaccgtca gggtgtccgt
     3001 ttgtacggca ttggcgatat aattatcaaa atcaaaaaag cggtcattga tcacggcgat
     3061 ctccgcattc gctttaaaaa gcatcatcgc catcatataa tcgcgcagat ctttattgcc
     3121 catccaattc ttttttaaaa tagaagacga cgggcccaat ccatacgcgg tttcacagtg
     3181 ttcggcatag gccagttcca cttcttcttc aaacgccgcc tgcaccgccg gatccacgta
     3241 aatcgtttcg gaggccagtt cgatcacttc gacgctctgc acatcatgta ttttgcgatc
     3301 ttgcacatcc atcgtgatat gcggtatgtt gcgaccaaat ttatccaccg aaaatacaaa
     3361 cgtctcaaaa cgatcactga cgcgtaccga actaaaacga tccggtgcga tatagctgga
     3421 cttaccgata atcacgttga tttctggaaa ttcgcgagca aaatcattgt tctcctcttc
     3481 cgaaaggtgc gtcaaaagaa caatggcatc cgattgttcg cgcacttcgt ctatatacgc
     3541 acggatcgcc gcactgggat cggcaatgat aatgccggcc attttttcct caagtacact
     3601 ttgtgttatt gccggatccg tcacgccgat aataccaacg cgcagcccgg ctttttcgac
     3661 gatcttatac ggctgcaaat acggcagtga gtccgcgcct aaaacattgg cgccgagaat
     3721 ggacggatac gaagggtcat ccacatacgc tttgagcaca tcttttccaa aatcaaattc
     3781 gtgattaccc accaccatcg cgtcatagcc gatatcccgc attttatcca aaacaatgcg
     3841 accgtgcgaa gccgtcgtca aataataata ccccagcgca tcgccggtat cgaaaaggag
     3901 catattctga cgaccataag ccgattggag ctgggttatc gcagcctgac gttccaatat
     3961 cccgcccgat ttgccaaatt cgacattctc ttcaaaaaga taacctttgg tatcgccggt
     4021 agcgacaatg tgcagactct gcgtgacggc gggatgatgc gcaacattga gtaaaaggca
     4081 aatacaaaaa aat
//
LOCUS       DAOQGF010000193         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_11994_length_4078_cov_7.291822, whole genome shotgun sequence.
ACCESSION   DAOQGF010000193 DAOQGF010000000
VERSION     DAOQGF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11994_length_4078_cov_7.291822"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2288
                     /locus_tag="PK937_15470"
     CDS             <1..2288
                     /locus_tag="PK937_15470"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH34022.1"
                     /translation="VGSSGKGLFKFDFNSNTISQNKNHWSYAPYALKTDMVTALLVDQ
                     SSMLWVATANGALQKNAPDFYKFDRLTDLFDHISGEMYVNAILPDSSSVWIGHHGGLV
                     RINKKSIETWEDIFPASHQSIRSVTALWKDSDGALWVGTTTAGLIRIKNRIKTTFLIS
                     PKENTSYSFPISSIIRDKKGQLWVGTIDGGLNLFDEIDKSFIRYDPANSIMKSASVSC
                     LYESSDSTMWVGTYGGLLQISQEGPWKYFTHDLKNPMSISHNYVYSVFCDQNGRTWVG
                     TNNGLCYKEKNSDDFKVLNQKNGLPSDVIVGILGSSGGKIWVSTHNGISSFDPNQPTS
                     FRNYDKYDGLPGNMFNIGAAIQSRDGTLWFGSIQGAVCFKSDQLRDNLFEPQVQIVQA
                     KKFNQIISIDSDLIRLNYDENYLSISFSAMDFFAPNKNQYQYRLEPLEKAWHYAGNKG
                     EAQYTDLAPGSYTLTVRATNRDGIWSSQIGAVQVIIRPPFWKTRSFLFLVAMTAFLLI
                     WFVHRSMTKRAVDKSLEMERIRLTERERMREQISKDFHDELGHKLTKITLFSELSQRA
                     LHDNKTDSEKFLRKVADTSTSLSVTTRDFIWALDPGKDSLYDVIVNLRDFGFDLVQDT
                     EIVFEVGGLTEQLDRIRLTMEWRRHLTLIFKEAMNNALKHAKAKHIRLRVIIEGIHFA
                     IHLTDDGTGFSQESTRSTRNGLKNMQERAQKLYGLLSIESSESAGTHISFTGLLPTID
                     AEARNSQEKRDWITTIKGWFV"
     gene            2285..2902
                     /locus_tag="PK937_15475"
     CDS             2285..2902
                     /locus_tag="PK937_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH34023.1"
                     /translation="MIRLMIVEDDPDIREGLELIAIQTRGVKLVGAYNTAETAIEATE
                     KDKPDVLLLDIELPGINGIEAIPLFKKRNENTDILILTVHQDDQTVFDALCAGASGYL
                     IKTAHPNKIMDAVKEINSGGVPMSASIARLVIQSFQKKQNPVLTTRETDVLRFLCKGH
                     SYKMIADSMHVSWETVHSHIKSIYRKLNVNSKSEAVITALKDRII"
     gene            3043..>4078
                     /locus_tag="PK937_15480"
     CDS             3043..>4078
                     /locus_tag="PK937_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNH34024.1"
                     /translation="MRKILPLFWIILVMVWPDRSLLGQMRFVPITDTSNPINTDPLGP
                     AVNNYNGSAWIDFDGDGDLDLYAHIGRLYRNDGGNFVAVTNSPGSGLSNAAVGKGISF
                     GDVDNDGDLDAFVTSTRGSGLYLNNGGTYTRIATGSIGQANAGWAGALGDYDNDGLLD
                     IIIAAPLGFEGISSSNLLFHNDGNAQFTQIDTSVITLQTGPFTVPSWYDYDMDGDIDL
                     FIGSGPATGNAGPDFYYKNMLNETGTAYFRRMTDNFASELRDGQNVNWFDFDNDGDFD
                     FFVTNYGFGNNSIQPNHFYRNDAGTFTKISTGAISSDADVSLGNLWEDFDNDGDLDCF
                     ISNENGDSSRY"
BASE COUNT         1205 a          752 c          911 g         1210 t
ORIGIN      
        1 gggtcgggtc gagcggaaag ggtttgttca aatttgattt taattctaac accatatcac
       61 aaaataaaaa tcattggtct tatgcgccgt atgctctaaa aactgatatg gtgacggcct
      121 tgcttgtgga tcaatcctcc atgttgtggg ttgcaacggc caatggggca ttacaaaaaa
      181 atgcaccgga tttttataag tttgaccgac ttacagacct gtttgatcac atatctggtg
      241 aaatgtacgt caatgccatt ttgcctgatt cgtcgagcgt ttggataggg catcatggag
      301 gattggttcg aataaacaaa aaaagtattg aaacatggga agatattttt ccggcatctc
      361 atcaaagcat tcgttcggta actgcacttt ggaaagatag tgatggggcg ctatgggtag
      421 gcactacaac agccgggttg attcgtataa aaaacagaat taaaacaacg tttttaatat
      481 ctcctaaaga aaatactagt tattcttttc cgatatcttc catcattaga gataaaaagg
      541 gacaactttg ggttggtacg attgacggcg ggctgaacct tttcgatgaa atcgataaat
      601 catttattcg ttatgatcct gccaattcga tcatgaaatc agcctctgta agttgtttgt
      661 atgaatcgag cgattcgact atgtgggttg gaacctatgg cgggttattg caaatttcac
      721 aagaagggcc gtggaaatat tttacacatg atttaaagaa tccaatgtcc atttctcata
      781 attacgttta ttcggttttt tgcgatcaaa atggaagaac ctgggtcggt acaaacaatg
      841 ggctttgtta taaagaaaag aactctgacg atttcaaagt tcttaaccaa aagaacggat
      901 taccgagcga tgtaatcgtc ggtattttag gaagctctgg tggaaaaatt tgggtcagta
      961 cacataacgg catcagtagt tttgatccca atcaaccgac ttctttcaga aattacgata
     1021 aatatgacgg tttgccggga aatatgttta atatcggagc ggctatacaa agccgcgatg
     1081 gcacgttatg gtttggtagc atccaaggtg cagtttgttt taaatcagat caacttaggg
     1141 ataatttatt tgaaccacag gtgcagattg ttcaagccaa aaaattcaat caaataattt
     1201 cgattgattc ggatttgatt agactcaact acgatgaaaa ttatctttcg atttcctttt
     1261 ctgctatgga ttttttcgct ccgaacaaaa atcaatatca ataccgtctt gagcctttgg
     1321 aaaaggcttg gcattatgcg ggaaacaagg gtgaagcaca gtatacggac ctcgctccgg
     1381 gatcatacac acttaccgta cgcgctacga atcgagatgg gatttggagt tcgcagatcg
     1441 gtgcagtaca agttattatt cgaccgccat tttggaaaac aagaagcttt ttgttccttg
     1501 ttgcaatgac agctttcctg ttaatttggt ttgtgcaccg tagtatgacg aaacgcgctg
     1561 ttgacaaatc cctcgaaatg gagcgtatcc gtttaaccga acgagagcgc atgcgagaac
     1621 aaatatccaa agattttcat gatgaattgg ggcataagct tacaaaaata acgcttttta
     1681 gtgagttatc tcaacgtgct cttcatgata ataaaaccga ttcggaaaaa tttttacgaa
     1741 aagtggctga tacgtccact tctttatctg ttacaacccg agatttcatt tgggcattag
     1801 atccgggaaa agactcgttg tatgatgtga tagttaatct tcgtgatttt ggattcgatc
     1861 tcgtacaaga tactgagata gtttttgaag tcggcgggct tactgagcaa cttgatcgaa
     1921 tccgtttaac gatggaatgg cggcgtcatt tgaccttaat cttcaaagag gctatgaaca
     1981 atgccttaaa acatgcaaaa gcaaaacaca ttcgtcttcg tgtaattatc gaagggattc
     2041 actttgcgat tcatttaacc gatgatggta ccggcttttc acaggagagt actcgttcaa
     2101 cgcgcaatgg cttaaaaaat atgcaagaac gcgcccaaaa actatatggt ttactgagta
     2161 tagaatcaag cgaatcagcg ggtacgcaca tatcctttac cggtttatta ccaacaattg
     2221 atgccgaagc acgcaattcc caggaaaaac gtgattggat aactacaatc aagggatggt
     2281 ttgtatgata cgactcatga tcgttgaaga cgatcccgat atacgtgaag gattggagct
     2341 tatcgccatt cagacgcggg gtgtaaaact cgtcggagct tataacactg cagaaaccgc
     2401 tatagaagca actgaaaagg acaaaccaga tgtactttta cttgatattg aattgccggg
     2461 aataaacgga atagaggcta ttccgctttt taagaaacgt aacgaaaata ctgatatact
     2521 tattttaacc gttcatcagg acgatcaaac ggtatttgat gctttgtgtg ccggggcatc
     2581 aggttatctg atcaaaacag cccatcctaa taaaatcatg gatgcggtga aagaaataaa
     2641 ctccggtggc gtaccgatga gtgcgtctat cgcacggctc gtaattcaat cttttcaaaa
     2701 aaaacaaaat ccggttttaa caacacggga gacagatgta ttgcggtttc tttgtaaagg
     2761 ccacagttat aaaatgattg ctgatagtat gcacgtcagc tgggaaacgg tgcattcaca
     2821 tatcaaaagc atctatcgta aacttaatgt taactccaaa tccgaagctg tcatcacggc
     2881 gttaaaagat cgtataattt gagttctcgt cgataggttg ccgttcttta tttagtgtaa
     2941 atacttccac acattaattc accaatattg gttacatctt tacgcttgga acttccttag
     3001 gtttgtaaaa ggaaaattaa ttacaaacgt aggtgaggtg ttatgcgtaa aattttaccg
     3061 ttattctgga tcatattagt gatggtttgg ccggatcgtt ctttgttggg tcaaatgcgc
     3121 ttcgtaccca ttaccgatac atctaatccc atcaatacag atccgctagg acctgcagtg
     3181 aataactaca atggctccgc atggattgat tttgacggtg atggtgattt ggatttgtac
     3241 gcccacattg ggcggcttta tcgtaatgat ggaggaaatt tcgttgccgt gacgaatagc
     3301 cccggtagcg gtttatccaa tgcggcagtt ggtaagggga tttcttttgg cgacgtggat
     3361 aatgacggtg atcttgatgc tttcgtgact tccacgcgcg gttcagggtt atatttgaat
     3421 aacggcggca cttatacacg tattgcgaca ggatcgatag gtcaagctaa tgccggttgg
     3481 gccggcgctt tgggcgatta tgataatgac ggcttattgg atattatcat tgctgcgccg
     3541 cttggttttg aaggtatttc cagtagcaat cttctttttc ataatgacgg aaacgctcaa
     3601 ttcacgcaaa ttgacacatc cgtgattacg ttacaaacgg gtccgtttac cgttcccagt
     3661 tggtatgatt atgatatgga cggagatatt gatttgttta ttggaagcgg tccggcaacc
     3721 ggaaacgccg gtcccgactt ttattataaa aacatgctta atgaaacagg aacggcatat
     3781 ttcagaagaa tgaccgataa ttttgctagc gaacttcgag acggtcaaaa tgtaaactgg
     3841 tttgattttg acaatgacgg tgattttgat ttttttgtaa caaattatgg gttcggaaat
     3901 aattcaattc aaccgaatca tttttataga aatgatgcgg gtacgttcac aaaaatatct
     3961 accggcgcta tttcatccga tgcggacgta tccttgggca atctttggga agactttgat
     4021 aatgacgggg acttggactg ctttatttca aatgaaaacg gagatagcag ccgatatt
//
LOCUS       DAOQGF010000194         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_11998_length_4076_cov_6.351156, whole genome shotgun sequence.
ACCESSION   DAOQGF010000194 DAOQGF010000000
VERSION     DAOQGF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11998_length_4076_cov_6.351156"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK937_15485"
     CDS             complement(<1..206)
                     /locus_tag="PK937_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34025.1"
                     /translation="MAFLYDILLQEFGKRAIAQVAVPNHITDNLKPGFGQRPYQIEAF
                     QRFILCHSEDFEGKPKRPFHLLYN"
     gene            complement(211..2232)
                     /locus_tag="PK937_15490"
     CDS             complement(211..2232)
                     /locus_tag="PK937_15490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH34026.1"
                     /translation="MKLYEALERQLKKEPNFVTDNGELKKWVVLNKAQNFDEELITLL
                     LKDETLKQKFFRTFSLSKGEVVTVFNQNLFAQFLEQKNYLNDSYTQYKNKVGLTIDGK
                     YLKQRNEVALVWPFKDCILEGGQSREEDKREEIFFNEILAQDEITQLLEPKVLTNAKR
                     IDKDGEKPLDQFNRNENHTITDNLIIKGNNLLALHTLKEEFAGKVKLIYIDPPYNTGK
                     DSFNYNDNFKHSSWLTFMKNRLEIAKQLLDDKGVLIVQSDDKEQAYLKVLLDEVMRPE
                     QFETSFYVQVRYGNKTLSEDNDFQKVMEIAHVYSKNTIKFKPNKIKEDYGLDKFKYEI
                     KELESGEKINIGGKEVEIFKEHQYEIKEVAPHINGLKETWATGSLIRQGGTAAEFLSK
                     YLIERKQTDGLKVLYKVYGMGEDGLGYRYISGPKKDEAFRGKFYSGVPASIRTGVEKG
                     EYQKEKPIPNFLYNFLQYESDFGNCRHEGGVDIEGGKKPEMLLSFFIEYFTKPNEIVL
                     DYHLGSGTTCAAAHKMNRQYIGVEQLDFGQNDSVVRLKNVINSDSSGISTEVNWKGGG
                     SFVYLELKKYNQTFIEQIEAAKDGKALLKVWEQMKTKSFLNYNVDIKKQDEHLEEFKA
                     LSLAEQKQHLCELLDKNQLYVNLSSLNDADFACTEEEKKITKDFYQIKK"
     gene            complement(2275..3789)
                     /gene="hutH"
                     /locus_tag="PK937_15495"
     CDS             complement(2275..3789)
                     /gene="hutH"
                     /locus_tag="PK937_15495"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNH34027.1"
                     /translation="MTEFAIHGDNLSIAVISEILKNKPRVVLTESAKQTINRGRRTIE
                     DIVSSGRVVYGVNTGFGKFSDVRISDDQLEILQENLVRSHACGVGEPFPADIAKVMLL
                     LKANGLAKGYSGVRLEIVETLLAMFNADVLPVIPQQGSVGSSGDLAPLAHLTLVLMGE
                     GEAWFQGQRLPGGEAMKRAGIKPVRLRSKEGLAVLNGTQTMTATAVVNLIRAKNCMEA
                     AEICGAMSMEVLLCTPTAFDPRIQDVRNQTGQIESARRLRDLVAGSPLIASHADCKKV
                     QDAYSVRCMPQVHGATRDAIAYVQQIVEREINAATDNPLIFSEDGDVLSGGNFHGQPV
                     ALVMDFLAIAVSEIGNISERRTAWMMDGHLSDGLPPFLAKDGGLNSGYMIAQYAAAAL
                     VSENKSLSHPASVDSVPTSANKEDHVSMGTIGARKAYRIIENVENILAIEWLCAAQAG
                     DFRKPIAFAGKTQKAYDLLRKYVPTLDKDRNTSVDIQTANAQLQSGAMVNAVWG"
     gene            complement(3798..>4076)
                     /locus_tag="PK937_15500"
     CDS             complement(3798..>4076)
                     /locus_tag="PK937_15500"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNH34028.1"
                     /translation="IPGFGGVLYAILMFGYVSFATAFSFLDYVLENRRFGFREKLRFV
                     MTNRAFCFGFGGVSALLLMIPFINVLFIPVLAVSATLMYIREPMQQKR"
BASE COUNT         1041 a          991 c          763 g         1281 t
ORIGIN      
        1 atattgtaaa gcaagtgaaa cggtcttttg ggtttgcctt caaaatcttc tgaatggcaa
       61 agaatgaaac gctgaaatgc ttcaatctga taaggtcgct gaccaaatcc gggttttaaa
      121 ttgtcggtta tgtgattagg aacggcaact tgtgctatgg ctcttttacc aaattcctgc
      181 aacaatatgt catataaaaa cgccatttgc ttacttctta atttggtaaa aatctttggt
      241 aattttcttt tcttcttcgg tgcaggcaaa gtcggcatca ttgagtgaag aaagattgac
      301 atacaattga ttcttgtcta atagttcaca caggtgctgt ttctgctcgg caaggctcaa
      361 ggctttaaat tcttccaaat gctcatcctg cttttttata tccacattgt aattcaaaaa
      421 acttttggtt ttcatctgct cccaaacttt caaaagtgct ttgccgtctt ttgctgcttc
      481 aatctgttca ataaaagttt gattgtattt tttgagttcg agataaacaa atgagccccc
      541 gcctttccaa ttcacttctg tactaatacc acttgaatca gaatttataa cattttttag
      601 tcttacaaca ctatcgtttt gtccaaaatc cagttgctct acgcctatat attgtctatt
      661 catcttatgt gccgcagcac aagtagttcc acttcccaag tggtaatcca atactatctc
      721 attaggtttc gtaaaatatt ctataaaaaa ggacaatagc atttcaggtt ttttacctcc
      781 ttcaatatct acacctcctt cgtgtctaca attaccaaag tcactttcgt actgtaaaaa
      841 attataaaga aaattaggta tgggcttctc tttttgatat tcaccttttt cgacaccagt
      901 tcttatactt gcaggcacac cagaataaaa cttacccctg aacgcctcat ctttttttgg
      961 tcccgatata tatctatatc ccaaaccatc ttcacccata ccgtatactt tatatagcac
     1021 cttcagccca tcggtttgtt tccgctctat tagatatttg cttaaaaatt cagctgcggt
     1081 tccaccttgc cttattaaac tacctgtagc ccatgtttct ttaagcccat taatatgtgg
     1141 ggcaacttct tttatttcat attgatgctc cttgaaaatt tctacttcct tacctccaat
     1201 attgattttt tcgccactct ctagttcttt tatttcatac ttgaacttat ctaatccata
     1261 atcttcttta attttattgg gtttgaattt tatggtgttt ttagaataaa catgagcaat
     1321 ttccattact ttctgaaaat cattatcctc agaaagcgtt ttgttaccat accttacttg
     1381 aacataaaac gaagtttcaa attgttctgg tctcattact tcatccaaca agacttttag
     1441 atatgcttgt tccttatcat cagactgaac aattaaaaca cctttgtcat ccagtaattg
     1501 ttttgctatt tccaatctat tcttcataaa agttagccaa gaagaatgct taaaattatc
     1561 attataatta aatgagtcct tccccgtatt atatggaggg tctatgtata tcagctttac
     1621 ttttcctgca aattcttctt tcagtgtatg caaggctaat aggttgttgc ccttaataat
     1681 caggttgtca gttatcgtgt ggttttcatt tcggttaaat tggtcaagcg gcttttctcc
     1741 gtctttgtca atgcgtttag cattggtcaa tacttttggt tctaagagtt gggtaatttc
     1801 gtcttgtgct aaaatctcgt tgaagaaaat ttcttctctt ttatcttctt cacggctttg
     1861 tccgccttcc aaaatgcagt ctttgaatgg ccaaaccaaa gccacttcgt tgcgttgttt
     1921 caggtatttg ccgtcaatgg tcagtccgac cttgtttttg tattgggtgt agctgtcgtt
     1981 caggtagttc ttctgctcca aaaactgcgc aaacaagttt tggttgaaaa ctgtaacgac
     2041 ctcacctttc gacaagctaa aagttcggaa aaatttttgt ttcaacgttt catcttttaa
     2101 taaaagcgtt ataagttctt catcaaagtt ttgagcctta ttcagcacca cccatttttt
     2161 cagctctccg ttgtccgtca cgaagttggg ctcttttttc agttgacgtt ctaatgcttc
     2221 atagagtttc atacagctat cctatgttaa acaatcttta tcgtctgata acccctaccc
     2281 ccacaccgca tttaccatag cacccgactg taactgcgca ttggccgttt gaatatccac
     2341 actggtattg cggtctttat ccaaagtcgg cacatatttc cggagtaagt cgtatgcttt
     2401 ttgcgttttg ccggcaaatg cgatcggctt gcgaaaatcg cccgcctggg ccgcgcacag
     2461 ccattcgatg gctaaaatgt tttcgacgtt ttcgataatc cggtaggctt tgcgtgcgcc
     2521 gatagtaccc atgctgacgt gatcttcttt attggccgaa gtcggtacgg agtctacact
     2581 ggccggatgg ctcaacgatt tattctccga tacgagcgct gcggccgcgt attgtgcgat
     2641 catatagccg gagttgagcc cgccatcttt tgccaaaaac ggcggcaaac cgtcgctcaa
     2701 atgcccgtcc atcatccatg ccgtgcgacg ttcggaaata ttaccgattt cactcacggc
     2761 tatcgccaga aaatccatta cgagcgcgac cggctggcca tgaaaattac cacccgataa
     2821 cacatcgccg tcttccgaaa aaatcagcgg attatctgtc gccgcattga tctcccgttc
     2881 gacgatctgt tgtacatacg cgatcgcatc ccgcgtcgcg ccatgaacct gcggcatgca
     2941 ccgcacgctg tacgcatctt gcactttttt acaatcggca tgggacgcga tcagaggact
     3001 gcccgcgacc agatctcgca gacgacgcgc ggactcgatc tgaccggttt gatttcgcac
     3061 atcctggata cgcggatcaa acgccgtcgg cgtacacagc aaaacttcca tggacatggc
     3121 gccgcagatt tcagccgctt ccatacaatt ttttgcacgt atgagattga cgaccgccgt
     3181 agccgtcatc gtttgcgtac cattgagcac agccaggcct tctttggagc gaagccgtac
     3241 cggctttatt cctgcgcgct tcatagcttc gccgccgggc agacgttgcc cctgaaacca
     3301 agcttcgcct tcgcccatca acaccaaagt caaatgcgcc agcggcgcca gatctccgct
     3361 cgaaccgacg gaaccttgtt gtggtatcac cggtaggacg tccgcattaa acatggctaa
     3421 taatgtttcg acgatttcca atcgcacgcc ggaataccct ttggctaatc cgttggcttt
     3481 gagaagtaac atcacttttg cgatgtctgc cggaaaaggt tcaccgacgc cgcaggcatg
     3541 gctgcgcacc aggttttcct gtaaaatttc aagctgatcg tcggaaatgc gtacgtcgct
     3601 gaattttccg aatcccgtat tcacaccgta caccacacgc cccgagctga caatgtcttc
     3661 gatcgtccgg cgtccccggt taatagtttg tttagcggat tcggtcaata ccacgcgggg
     3721 tttgtttttc agaatttctg aaatcaccgc aatactcaga ttgtctccat ggatggcaaa
     3781 ttctgtcata gctccattca ccgtttttgt tgcataggtt cacgtatata catgagcgtg
     3841 gcgctaacgg ccaacacagg tataaaaagt acattgataa aaggaatcat caaaagcaga
     3901 gcggaaacac cgccaaaacc aaaacagaag gcgcgattgg tcattacaaa acgcagtttt
     3961 tcgcgaaaac caaatcggcg gttttccaat acataatcca aaaaactgaa tgctgtcgcg
     4021 aaactgacat acccgaacat cagtatcgca tagagcacgc ctccgaagcc gggtat
//
LOCUS       DAOQGF010000195         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_12164_length_4044_cov_4.783404, whole genome shotgun sequence.
ACCESSION   DAOQGF010000195 DAOQGF010000000
VERSION     DAOQGF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12164_length_4044_cov_4.783404"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PK937_15505"
     CDS             complement(<1..342)
                     /locus_tag="PK937_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNH34029.1"
                     /translation="MAKGKVIGIDLGTTNSCVSIMEGGQPVVIANSEGGRTTPSVVGF
                     TKNGERLIGNPAKRQSITNPKNTIFSIKRFMGRRYDEVTSERGRVPYEVIAGDNNTAR
                     VKVADNMYSPPE"
     gene            complement(641..1141)
                     /locus_tag="PK937_15510"
     CDS             complement(641..1141)
                     /locus_tag="PK937_15510"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH34030.1"
                     /translation="MNLWIRMLRVFWKSFFCKKKISLFDESRLSFRVWPHDCDINMHL
                     NNGRYLTLMDLGRTYLMAEMQMLWKLPKRKWFPVLGGVEIQFLKSIDPFQKFDIVTHV
                     LTWDAKWIYVEQRFERNGVVLAKAHLRALFLGPKGRVATDAIIALAELGSVEAPLHPV
                     LQKWKD"
     gene            complement(1220..1807)
                     /locus_tag="PK937_15515"
     CDS             complement(1220..1807)
                     /locus_tag="PK937_15515"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNH34031.1"
                     /translation="MAKKKSAPKKAAKKVVKKAVKKVAKAAPKKSTKKVAKKAAGVKK
                     QKLTPDQLHAKVLGKVETYIKRIYGKSYEKHDDGRFLVFEGSTVVQTVLRPWHAGDIV
                     VESFAYVVQDARLTDELMKFLLRENATLHFGSFGLSFDGTIIFGHSLAGANLDENEFS
                     ASIKSVARIADHYDNKIVEIAGGMTAREAMKKGIM"
     gene            complement(1905..2846)
                     /gene="queG"
                     /locus_tag="PK937_15520"
     CDS             complement(1905..2846)
                     /gene="queG"
                     /locus_tag="PK937_15520"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNH34032.1"
                     /translation="MADKKLKEFICEEARNLGFDKVGFATAAHVTQAEYLKRWLNQGY
                     HASMQWMNNHLEKRIDPRMLFPGARSVIVVALNYHQPQARTVIENSGKISQYAWGEDY
                     HEVLKDKLYMLLNKLCLHDTQIEGKVCVDTAPMMDKYWAVQAGIGWQGKHSNIISREL
                     GSWFFIGSLIVNVDIEPDMPIADFCGSCTRCIDACPTQAIIHPYVVDSGRCISYYTIE
                     HRREHLPESLVAHLQNWIYGCDVCQDVCPWNIKFEKPTREARFTPRMENINVSLQTWQ
                     NLTEHDFRLKFRKSAVKRTKFAGLLRNIYAALEPKAY"
     gene            complement(2944..3846)
                     /locus_tag="PK937_15525"
     CDS             complement(2944..3846)
                     /locus_tag="PK937_15525"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNH34033.1"
                     /translation="MNRSILFCLAMYIVACGEKVNFADKSEPHSQAGAAMRNIQAPIN
                     EQSADVSPIADRHERSSTASLYAAEKKQDRIIIKNGSLQIQVQNYDETVSKLRTMVTA
                     WGGFVAHSSTSVPYADVKEGNWTIRIPSEHFDTAMTGVKTLGQKTERESVEGQDITEE
                     FYDLEARLRNKRLEEEQVQAILRRAGTISDVLEVQRELNRIREEIERAEGRKQFLSDR
                     VKYSTINIQMHEAYPVTVSASGGFWATIAEGFADGFKGFANVLSGTITVLIAGTPVFI
                     VFSLVIRFIFLRLRRQRENPKISG"
BASE COUNT         1116 a          917 c          766 g         1245 t
ORIGIN      
        1 ttccggcggt gaatacatat tatccgctac cttgacgcgt gccgtattat tatcgccggc
       61 tatcacttcg taaggtacac gtccacgctc actggttact tcgtcataac ggcgacccat
      121 aaaacgtttg attgaaaaaa tcgtattttt gggattggtt atggattgac gtttggccgg
      181 attaccgatc aaacgctcac cgtttttggt aaaacccaca accgacggtg ttgtacgacc
      241 accttcagaa tttgcaatga caaccggttg accgccttcc ataatcgaaa cacatgaatt
      301 cgttgttccg agatcgattc cgattacttt accctttgcc ataccattac tccttttatt
      361 ttcaaatgtt tattttaagt aaactttttt tcctgacatt aattcagcaa agcttgtgcc
      421 gctaattttg atactgattt taaataactt acgattaaat ataatgaaaa attgtcatga
      481 aaatttcatt caatgacaaa acggcatcgt gtcgcttgct ttattgacaa gacgtcatgc
      541 cgcttaattt gaagcattta gattggaatg aggaagcttt agtacgacga tagtaatgga
      601 tacattatat tacaattaca atttcgataa atttagttat ttaatctttc catttttgta
      661 aaactggatg caaaggcgct tcgacagaac ctaattcggc taaagcaatt atggcatccg
      721 tagcaacacg gcctttcgga cctagaaata atgcacgcag atgggctttg gccaaaacta
      781 cgccgtttct ttcgaaacgc tgttccacat aaatccattt ggcatcccaa gtaagaacgt
      841 gtgtcacaat atcaaatttt tgaaaaggat ctattgattt cagaaactgg atttccactc
      901 cgcctaatac gggaaaccat tttcttttgg gtaatttcca tagcatttgc atctccgcca
      961 tcagatatgt acgcccaagg tccatgagtg taaggtagcg cccgttattg aggtgcatat
     1021 taatgtcgca gtcatgtggc cacactctaa aactcaaccg actttcatcg aaaaggctta
     1081 ttttcttttt gcaaaaaaaa gatttccaaa acacacgcaa catacgaatc cacaaattca
     1141 tgagctatcc cctgatatat aaaaatcaaa accagcgccg gagacgctgg tttttttcaa
     1201 cgtaatattt tatttcagat cacatgatgc cttttttcat tgcttcacgt gcagtcatac
     1261 cgccggcaat ttcgacgatt ttattatcat agtgatccgc gatacgtgct acggatttga
     1321 tcgaagcgga aaattcgttt tcatccagat tggcaccggc cagcgaatga ccgaagataa
     1381 tcgttccgtc aaatgacaaa ccgaatgaac cgaagtgtaa tgtcgcattt tcacgcaaca
     1441 aaaacttcat cagttcgtcc gttaagcgcg catcctgaac cacataagca aacgattcga
     1501 cgacaatatc accggcgtgc cacgggcgca acactgtttg aacaaccgta gagccctcaa
     1561 atacgaggaa tcggccgtca tcgtgttttt catagctctt accatagata cgtttgatat
     1621 atgtctctac tttacccaat actttggcgt gtaattgatc cggagttaac ttttgctttt
     1681 tgactccggc tgctttttta gcaactttct tcgttgattt ttttggtgca gccttagcta
     1741 cttttttgac tgcttttttt acgacttttt tagccgcttt ttttggcgct gattttttct
     1801 ttgccatgag atcgtgcctc cgccgtattg aagtaattgt attctaaaaa ttattgacct
     1861 gaaaacaatg attggatcaa aaatcgaaaa aaaaacaaac aaagtcaata cgctttcggt
     1921 tccagagccg cataaatatt tcttagtaac cctgcaaatt tagtgcgctt gaccgcgctt
     1981 ttgcggaatt ttaagcgaaa atcatgttca gtcaagtttt gccaagtctg taacgaaaca
     2041 ttgatgtttt ccatacgtgg agtaaaacgg gcttcccgtg taggtttttc aaatttgata
     2101 ttccacgggc aaacatcctg acacacgtcg cagccatata tccagttttg caaatgtgca
     2161 acaaggctct cgggcaagtg ctctcttcgg tgttcgatcg tatagtatga aatgcatcgc
     2221 ccggaatcta caacatacgg atggataatc gcttgcgtgg gacatgcatc aatgcatcgc
     2281 gtacatgatc cgcaaaaatc cgcgatcggc atatctggct ctatatcaac attgacgatt
     2341 agtgatccga taaaaaacca agaacccaat tcacgcgaaa taatattgga atgtttgcct
     2401 tgccatccga tacctgcctg tacggcccaa tatttatcca tcatcggcgc cgtatcaacg
     2461 cataccttgc cttcgatctg cgtatcgtgc aaacaaagtt tatttagtag catatacaac
     2521 ttatctttta acacctcatg ataatcttcg ccccatgcgt actgggaaat ttttccggaa
     2581 ttttctatca cggtacgtgc ttgaggctgg tggtagttaa gtgccacgac gattacggaa
     2641 cgggcacccg gaaacaacat gcgcggatca atgcgttttt ctaagtggtt attcatccac
     2701 tgcatggacg catggtaacc ttgattcaac caacgcttta gatattccgc ctgtgtaacg
     2761 tgagccgctg tagcgaatcc gactttatca aaccccaaat tccgggcttc ttcacatata
     2821 aactctttta attttttatc ggccataagt tttatagcta ttgttcatct aatatacacc
     2881 gatactaaaa aaaacgcata tgaatttcat atgcgataaa tatgctaata tatgaatcgg
     2941 tgctcatccg gatatttttg gattttccct ttgccggcgc aaacgcaaaa aaataaacct
     3001 gattactaga ctgaaaacaa taaaaacagg tgtaccggca atcaagacgg tgatcgtacc
     3061 gctgaggaca ttggcaaagc ctttaaaacc gtccgcaaaa ccttcagcga tggttgccca
     3121 aaatcctccg ctcgcgctga ccgtgacggg ataagcttcg tgcatttgta tattgatcgt
     3181 agaatatttg acccggtccg agagaaattg tttgcggcct tccgctcgct caatttcttc
     3241 acgaattcgg tttaattcac gttgcacttc taatacatcc gaaatcgtac ccgcacgccg
     3301 aagaattgct tgtacttgct cttcttcaag gcgtttattg cgaagccgcg cttccagatc
     3361 gtaaaattcc tctgttatat cttgtccttc aacgctttct ctttctgttt tttgacctaa
     3421 agttttaacc ccggtcatgg ccgtatcaaa gtgttcactc ggaatacgta tggtccagtt
     3481 tccctctttg acatcggcgt aaggtacaga cgtagatgaa tgtgcaacaa atcctcccca
     3541 cgccgtaacc atggtcctaa gcttggaaac tgtttcgtca tagttttgta cttgtatctg
     3601 aagtgaaccg ttctttatga taatacgatc ctgctttttt tccgcagcgt acaacgatgc
     3661 ggttgagcta cgttcatggc gatcagctat cggtgacaca tccgcacttt gttcatttat
     3721 tggtgcctga atatttcgca tcgctgcacc ggcttgcgag tgcggttcac tcttatcggc
     3781 aaaattcact ttttcgccgc atgcgactat atacatagcc aaacaaaaga gaatgctgcg
     3841 attcatgcta tctccacggt tttatgaata accgatttta tctatttaac gaatatgttg
     3901 aattcttgat gataatcgca cttatgtttg cattttgtat tcgaaacgag ctacgcagta
     3961 aggtgtctga aaagagcgat tttcgttaat taaattggtg ttatgggctt agtgtgcttt
     4021 tcaaaaaaac caattcccac atac
//
LOCUS       DAOQGF010000196         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_12336_length_4009_cov_5.425645, whole genome shotgun sequence.
ACCESSION   DAOQGF010000196 DAOQGF010000000
VERSION     DAOQGF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12336_length_4009_cov_5.425645"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="PK937_15530"
     CDS             complement(<1..1639)
                     /locus_tag="PK937_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34034.1"
                     /translation="MAGVYKFSDMRSPLLFVSLLFSALPAFAQTSVAELKNFEMDKIY
                     VKGFSVSKNSSVHIKGIGVMSDHEKWYRWNPMIAYGWIINADTRELVWTMDPSNVTSN
                     WNSRNIVYEGNLTLAPGNYEAYYTTTGQKVIRISRSQSYFNQFFKNLANVFVDDADLY
                     ENADEWLLQITSDERPSNFGSYDGALPKTVFLNLCGARDSDYREKGFTLERDIKVKVY
                     CNGEGQDRRMYDYGWIAEDKSSKTVWEMRFDNTEHGGGAEKNRLYRGIITLPAGNYVA
                     TFLTDDSHSFMDWNMQPPYDPSSWGMMLSVVDERDLDFVREYKKAKKQEILSITGVGD
                     EQFRSEGLSLSRKTDLHIFALGEGRENRMYDYGWIVNARDGNTIWEMRFRDTKFAGGA
                     DKNRLFDGVVSLPAGEYMVYYRTDGSHSAEDWNDDPPYNQQKWGITLSLVNNSDASSI
                     SKLNELPEDNNILAQIVRVGDDEYETRDFSLNRATEVRIQCIGEGRSGRMYDYGWIKN
                     AETGQTVWKMLYEDTEPAGGARKNRSFNGVVLLAPGNY"
     gene            complement(1795..2550)
                     /locus_tag="PK937_15535"
     CDS             complement(1795..2550)
                     /locus_tag="PK937_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNH34035.1"
                     /translation="MEQKKMTLHLKIWRQKNMQSAGKMVDYTLDNVSTDMSFLEMLDV
                     LNERIIKNGEDPIEYDNDCREGICGMCSMVINGVAHGPEKGTATCQLHMRKFKDGDHI
                     TVEPWRASPFPVVKDLATDRSAFDAIIAAGGYTSCRVGSAAEANALLVPKDMADKAMD
                     AAACIGCGACVAACPNGSASLFVSAKIAQFTYLPQGHAERKQRVLNMVNKMDELGFGD
                     CSNHGECEAVCPKEISITHIAIMRKEYLKAALS"
     gene            complement(2599..>4009)
                     /gene="sdhA"
                     /locus_tag="PK937_15540"
     CDS             complement(2599..>4009)
                     /gene="sdhA"
                     /locus_tag="PK937_15540"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HNH34036.1"
                     /translation="GAYGSLMRQVNEGAVKMFPRREMLDLVVIDGQARGIVVRNLVTG
                     AIESYAGDAVLLCTGGYGNVFYLSTNAKNSNVTASWRCYKKGAFFGNPCYTQIHPTCI
                     PQSGDYQAKLTLMSESLRNDGRVWVPKTKGDKRPPEQIPENERDYYLERKYPSFGNLV
                     PRDVASRNAKSVCDEGRGVGETGLAVYLDFSDAIKRLGEDDIREKYGNLFDMYERITG
                     DNPYKTPMRIYPAVHYTMGGLWVDYNLMSTVPGLFVLGEANFSDHGANRLGASALMQG
                     LADGYFVIPYTLANYLASSSFNKITTDHQAFKTAATAVTEQTKKLLAIKGKKTVLEFH
                     RELGKIMWDEVGMARNKAGLERALSEIPKLREEFWKNVNVPGEGELLNKNLEFAGRVA
                     DFLELGELMAMDALQREESCGGHFREEHQTPDGEAKRNDEQFAYVAAWEYAGADKAPI
                     LNKEELTFENVHLAQRSYK"
BASE COUNT          994 a          979 c          938 g         1098 t
ORIGIN      
        1 tataatttcc cggagccaat aagaccacac cattaaatga tctgtttttt cgtgcgccac
       61 cggccggctc ggtatcttca tacagcattt tccaaacagt ctgaccggtt tcagcgtttt
      121 ttatccatcc ataatcgtac atccttccgg aacgtccttc accgatgcat tgaatgcgca
      181 cttcggtagc gcgattcagc gaaaagtcgc gtgtttcgta ttcgtcatct ccgacgcgaa
      241 cgatctgtgc gaggatatta ttatcttccg gcaattcatt aagtttactg atggacgatg
      301 catcgctgtt attaaccagg cttaacgtaa tgccccattt ttgttgattg tatggcggat
      361 catcgttcca atcttcagcc gaatgcgaac cgtcggtgcg gtaatacacc atatattcac
      421 cggcaggtaa cgatacgact ccatcaaata aacggttttt gtcggcgcca ccggcgaact
      481 tggtatcgcg gaatcgcatt tcccatattg tgttgccgtc gcgcgcattg acgatccatc
      541 cgtagtcgta catccggttt tcacggcctt ctcccagggc aaaaatatgt agatccgttt
      601 tacgactgag cgataagcct tcagaacgga actgttcatc gccgacaccg gtaatagaaa
      661 gaatttcctg tttcttggct tttttgtatt cgcgtacaaa atccaaatcg cgctcgtcaa
      721 cgacactcag catcataccc catgaagagg gatcgtacgg cggttgcata ttccaatcca
      781 taaacgaatg cgaatcatcc gttaggaatg ttgcaacata gttgccggcg ggcaaggtga
      841 tgatgccgcg atacaaacga tttttttcag cgccaccgcc gtgttcggta ttatcaaaac
      901 gcatttccca tacggtcttt gaagatttat cctcagcgat ccagccataa tcatacatgc
      961 gccggtcctg gccttcaccg ttgcaataca ctttgacctt tatgtcgcgc tccagcgtaa
     1021 aacctttttc ccggtaatcg ctgtcgcggg cgccgcaaag gtttaggaaa acggttttgg
     1081 gtaaagcgcc gtcgtatgaa ccaaaattgg atggcctttc atccgatgtg atttgtaata
     1141 accactcatc cgcgttttcg tacaaatcgg catcgtcaac aaaaacattg gccagatttt
     1201 tgaagaattg attgaaataa ctttgtgagc gggagatacg aataaccttt tggcccgtcg
     1261 tggtgtaata ggcttcataa ttacccggcg ctaacgtcag attgccctcg taaactatgt
     1321 tgcgcgagtt ccaattgctg gtgacgttgg aaggatccat ggtccaaacc aattcacggg
     1381 tatccgcgtt gatgatccat ccgtaagcga tcatgggatt ccagcgatac catttttcgt
     1441 gatccgacat cacaccgata cctttgatat gcacggatga gtttttggaa acgctgaatc
     1501 ctttgacata gattttatcc atctcaaagt ttttcagttc cgctacggat gtttgagcaa
     1561 acgcgggtaa tgcggaaaaa agcagggaaa caaaaagcag gggactgcgc atgtctgaga
     1621 atttataaac acctgccata gtacaaacca cctttcatta agggtgtaac caataatgaa
     1681 tacgatgctt atggtttaag gtttcacaaa aaaattaaaa aattataaaa atagtattac
     1741 aaaaaaggcg agcatgcatc gctcgccttt tcaaaggggc aagattatta gcatttagct
     1801 cagtgcggct ttaagatatt ctttgcgcat gatagctata tgagtgatgg atatttcttt
     1861 cgggcaaaca gcttcgcatt caccgtgatt ggagcagtcg ccaaaaccga gttcatccat
     1921 tttattaacc atattgagta cgcgttgctt ccgttcggcg tgaccttggg gcagataagt
     1981 aaactgcgcg attttagcgg atacaaaaag ggaagcggag ccgttggggc aggccgctac
     2041 gcaagcgccg caaccgatac atgcggccgc atccattgct ttatccgcca tatctttggg
     2101 aaccagtaac gcattggctt cggccgcgga gccaacacgg cacgaggtat aaccaccggc
     2161 ggcaataatc gcatcaaaag ccgaacgatc cgtggctaaa tctttaacaa cgggaaaggg
     2221 tgatgcgcgc caaggttcta ccgtgatgtg gtcgccgtcc ttaaatttgc gcatgtggag
     2281 ctggcaggtg gccgtgcctt tttcaggacc gtgggcaaca ccgttgatca ccatgctgca
     2341 cataccgcaa ataccttcac ggcaatcatt atcgtattcg atggggtcct caccgttttt
     2401 gatgatgcgt tcattgagaa cgtccagcat ttcgagaaac gacatatcgg ttgatacatt
     2461 gtccaacgta taatccacca ttttaccggc gctttgcatg tttttctggc gccagatctt
     2521 caggtgaagg gtcattttct tttgttccat aatttcctct tatctattaa gtgccgcgtt
     2581 gcggcgcttc ggttccgatt atttatagct ccgttgggca agatgaacgt tttcaaacgt
     2641 cagttcttct ttgttgagta tcggcgcttt gtcggcaccg gcatattccc atgccgcgac
     2701 gtaagcaaac tgttcgtcat tacgtttggc ctcaccatcc ggtgtctgat gttcttcacg
     2761 gaagtggcct ccgcatgatt cctcgcgttg cagtgcgtcc attgccatga gctcgccaag
     2821 ttccaagaaa tcagcaacac gtcccgcaaa ttcgaggttt ttgttgagta attcaccttc
     2881 gccgggaacg ttgacatttt tccaaaattc ttcacggagt tttgggattt cacttaaagc
     2941 gcgttccaat cctgctttat tgcgagccat accgacttcg tcccacatga ttttgccgag
     3001 ttcgcgatga aattccaaaa cggttttttt gcctttgatg gccaatagtt ttttcgtttg
     3061 ttcggttact gcggttgcgg ctgttttgaa cgcctggtga tccgtcgtga ttttattaaa
     3121 acttgacgag gcgagatagt tagccaaggt ataaggaatc acgaaatagc cgtcggcgag
     3181 tccttgcatc aaggcgctgg cgccaaggcg attggctccg tgatccgaga aattggcctc
     3241 acccaataca aacaagcccg gaacggtgga catcagatta taatccaccc aaaggccgcc
     3301 catcgtatag tgaacagcag gatatatacg catcggtgtc ttgtaaggat tgtcaccggt
     3361 gatacgttcg tacatatcaa aaaggttgcc gtatttttca cgaatatcgt cttcaccgag
     3421 gcgtttgatg gcatctgaaa aatcaagata tacggcgaga cctgtttcgc cgacaccgcg
     3481 tccttcatcg catacggatt tggcattacg ggatgccaca tcacgcggta caagatttcc
     3541 gaaactcggg tacttacgtt ccaaataata gtcgcgttca ttttcaggaa tttgttccgg
     3601 tggacgttta tcacctttgg ttttaggaac ccatacgcga ccgtcattac ggaggctttc
     3661 gctcatgagc gtcagtttgg cctgataatc gccactttgg gggatacagg tcgggtgaat
     3721 ctgtgtgtag cagggattgc cgaaaaacgc acctttttta tagcatcgcc acgacgcggt
     3781 gacgttggaa tttttagcat tggtcgaaag ataaaaaacg tttccgtagc cgccggtaca
     3841 caacagtacg gcgtcaccgg cataggattc gatcgcaccg gtcacgagat tgcggacgac
     3901 gatgccgcgg gcctgaccgt caatcaccac cagatccagc atttctcgac gggggaacat
     3961 tttgacggcg ccttcattga cctgacgcat cagcgaaccg taggcgcca
//
LOCUS       DAOQGF010000197         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_12683_length_3945_cov_7.340360, whole genome shotgun sequence.
ACCESSION   DAOQGF010000197 DAOQGF010000000
VERSION     DAOQGF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12683_length_3945_cov_7.340360"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..810
                     /locus_tag="PK937_15545"
     CDS             136..810
                     /locus_tag="PK937_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34037.1"
                     /translation="MNYLSHFAINQKKQVSPEFHLGAALPDLMSAINRTLRFQENAVR
                     AYIPHAHCPDVWHGVLNHFEADAAFHGSSFFVSCYETIRELLKENIAPQKNIRPFFLA
                     HIVVEILLDHVLSSNDTSLASRYYRNLERIVVPTIQTDVDRYFGNSTSEFTEIFDRFM
                     RFKFLYEYDQIKKMHEPINRMLRRTRQDGFAEHEIADLIAILETCKHIIVKALPEFEA
                     QFMPQD"
     gene            747..1730
                     /locus_tag="PK937_15550"
     CDS             747..1730
                     /locus_tag="PK937_15550"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH34038.1"
                     /translation="MQTYHSKSVAGIRGAIYAAGLICFLSAVISCDNSDTDHRIWYVA
                     DKRIYVYAIGESRDYYWVSAQEKILRISKNDGDTLSITYGTQGFPANAVGCFFPADSG
                     RIWMGTGGGGLLLVDTIVVRRYTTSNSQIPSNGVQAIAQSSDGVFWLATNGGLVKFDN
                     HNFTTFNTSNSGLPANALTSVGVGDDKSVWAGTWGFNLARFKENQWTIFTKNNSSLQT
                     AYIFTLLHTGKTLWVGEGGGINRYTASGWKNYNSKDTDLPQNDVTTIVVDKYGLWVGT
                     YAGGVGFLKSETWTVYNTTNSELPDDQVTAMFADRRGRLWVGFYRGLAVFQ"
     gene            1868..2935
                     /gene="rsgA"
                     /locus_tag="PK937_15555"
     CDS             1868..2935
                     /gene="rsgA"
                     /locus_tag="PK937_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNH34039.1"
                     /translation="MTLDHLGWQPFFDTAFKPYAGEFQVGRIAVQQTRVYELWTEAGV
                     ISAEMSGNLRKSAEIDSTLRPVVGDWVVIQLRLAERFAVIHHVLPRRTKFSRKVTEEV
                     TREQVIAANIDIAFLVSGLDQDFNLRRLERYLLTAQHSGAEPVIVLNKVDLCAESDAR
                     LSEVRAIAPSVPIITMSAMHKQGIELMNFYLGQGKTGCLLGSSGVGKSTIINALLDDD
                     VQSTRTTRKDMDRGRHTTTHRELFVLPGGGLLMDTPGMRELQLWTDESSLSGTFEDIE
                     TLAAACRFRDCRHESEPDCAVQAALADGSLDAARYKSYLKLRREIRYLELRQDNSAAR
                     LEKMRWKKIHAQHKKNNRSKN"
     gene            3053..3562
                     /locus_tag="PK937_15560"
     CDS             3053..3562
                     /locus_tag="PK937_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNH34040.1"
                     /translation="MKQKLESAFESIIFASRWIQAPLYAGLIVGGILYAYKFLNELIH
                     LCLTINEISESVLMLGVLTLVDITMVANLLVMVIIGGYSTFVSRMNIDEHEDKPEWLQ
                     KVDAGTLKVKLAGSLVGVSGIHLLQVFINIKNHNSEQVMWQTIIHVVFLFSAIMLAYS
                     EKLLHEKHG"
     gene            complement(3577..>3945)
                     /locus_tag="PK937_15565"
     CDS             complement(3577..>3945)
                     /locus_tag="PK937_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34041.1"
                     /translation="NTYRITGGPNTSINLDMDIVANYHMLFMKLKPMEVGEADSLTSK
                     KIELNAEEWGKRCITGDTWYKIKRRPDNAFGERNYEIIQPGFNGSSEFSFQSIITLGN
                     DGIIKDIRFNNHVTAKRIVP"
BASE COUNT         1060 a          791 c          959 g         1135 t
ORIGIN      
        1 tacgcataaa caatatcgtt atatgtgcgt catgacctta gccggaggat ttagcgctaa
       61 aaaaaataca atccataccc gcggcgaaaa tccggcggac ggcgatgcga cgggcgataa
      121 ataaagcgtt gatgaatgaa ttatctttcg cattttgcga taaatcaaaa aaaacaagta
      181 tcccctgaat ttcaccttgg cgcggcactg ccggacctga tgagtgcgat caaccgtact
      241 ttgcgttttc aggaaaacgc ggttcgtgct tatattcctc atgcgcattg cccggacgtt
      301 tggcatggcg tgctcaatca ctttgaggcc gatgcggctt ttcacggttc tagttttttt
      361 gtttcgtgtt acgaaaccat ccgcgagttg ctcaaagaga atattgcacc tcaaaaaaac
      421 atcagaccgt tttttctggc gcatattgtc gtggaaatat tactggatca tgttttgtcg
      481 tcaaacgata cctcgcttgc atcgcgctat tatcggaatc tggaacgtat cgttgtaccg
      541 acaatacaaa cggatgtgga tcgttatttt ggtaattcga catctgaatt tacagaaatt
      601 tttgatcggt ttatgcgttt taagttctta tacgaatacg atcaaatcaa aaaaatgcac
      661 gagccgatca accgcatgct tcggcgcaca cgacaagacg gttttgcaga acatgaaatt
      721 gcggatttga tagcgatctt agaaacatgc aaacatatca tagtaaaagc gttgccggaa
      781 ttcgaggcgc aatttatgcc gcaggattga tttgtttttt atcagctgtc ataagttgtg
      841 ataattcgga taccgatcac cgtatctggt acgtggcgga taagcggata tatgtgtatg
      901 cgatcggaga atcccgtgat tattattggg tatctgcgca ggaaaaaata ctccgcatta
      961 gtaaaaacga tggtgacacc ttgtctataa cgtatggtac acagggtttc ccggctaacg
     1021 cggtcggatg ttttttcccg gcggatagcg gtcgtatttg gatgggaaca ggcggcggcg
     1081 gtttgctatt ggtcgatacg atcgttgtgc gccgttatac gacatccaat tcgcaaattc
     1141 catcgaatgg cgttcaggct atcgctcaat catcggatgg tgttttttgg ctggctacca
     1201 atggcgggtt ggtaaaattt gataaccata attttacaac gttcaataca agtaactccg
     1261 gattacctgc aaatgctctg acatccgtag gtgtgggcga tgataagagc gtttgggccg
     1321 gtacatgggg tttcaatctt gcgcgtttta aagaaaatca atggacgata tttacgaaaa
     1381 ataattcgtc gcttcagacg gcttatattt ttacactgct tcataccggt aaaacgttat
     1441 gggtcggtga aggcggaggc atcaatcgtt atacagcctc gggatggaaa aattataaca
     1501 gcaaagatac tgatcttccg caaaatgatg taacgaccat tgttgtggat aagtatggtt
     1561 tgtgggtagg tacgtatgcc ggtggcgttg gttttttaaa aagtgaaacg tggacggtat
     1621 ataatactac gaatagcgaa cttcctgatg atcaagtaac ggctatgttt gccgatcgtc
     1681 gaggccgttt gtgggttggt ttttatcgcg ggttggctgt ttttcaataa aattgttaat
     1741 tatttatatc actttttaca caaaggagag tctatgattg ccgtcattgt tttcaagttt
     1801 catgaaaact tattaactac gtaacggagc tttgtactcc gttgctaaaa caatggagca
     1861 aaatcaaatg acgttagatc atttagggtg gcaacccttt tttgatacag cattcaaacc
     1921 ttacgccggt gaatttcagg tagggcgtat cgccgttcag cagacgcggg tttacgaatt
     1981 gtggacggaa gccggtgtta tttcggcaga aatgtcgggc aatcttcgca aatcggcgga
     2041 gattgattcc acattgcgac ctgtcgtcgg tgattgggtc gtgatccagt tacgtttggc
     2101 cgaacgtttt gccgtgatac atcatgttct cccgcgccga accaagtttt cgcgtaaagt
     2161 caccgaagag gtcacacgcg aacaggtgat cgcggcgaat attgacatag cttttttggt
     2221 aagcggtctg gatcaggatt ttaatctgcg ccgacttgag cggtatttgc ttactgcgca
     2281 gcatagcggt gcggaaccgg tgatcgtact caacaaagtc gatctttgtg cggaatcgga
     2341 tgctcggtta tccgaagtgc gggctattgc accgtccgtg cccatcataa cgatgagcgc
     2401 gatgcacaaa caaggcatcg agttgatgaa tttttacctt ggacaaggta aaaccggttg
     2461 cctactcgga tcctccggcg tcggtaaatc aacgatcatc aatgcgttgc ttgatgatga
     2521 tgtccaatca acacgcacta cacgcaaaga tatggatcgc ggaagacaca cgacgacgca
     2581 tcgtgaatta tttgttttac cgggcggcgg tttgctgatg gatacgccgg gtatgcgcga
     2641 attgcaatta tggacggatg aaagcagtct ttcaggaacg ttcgaagata tcgaaacgct
     2701 tgcggcggca tgccgctttc gggattgccg acacgagtca gaaccggatt gtgccgtaca
     2761 agcggcatta gccgatggat cgctggatgc ggcgcgatac aaaagctatc tgaaactgcg
     2821 aagagagatt cggtacttgg aattgcggca agataactcg gccgcacgat tggaaaaaat
     2881 gcggtggaaa aaaattcacg cccagcataa aaaaaacaat cgctctaaaa actaaacaaa
     2941 aaagcgatag cttgtcacag cccggttctg tagtaataca acccgggctt gttttttaat
     3001 cgaatgtcgt taaattgacc acgcaaaata attacaggag aagttttttg ctatgaaaca
     3061 aaaattggaa tccgcttttg aaagtatcat ttttgccagc cgatggatac aagcgccgct
     3121 ttatgccggg ttaattgtcg gcggaatttt atatgcatat aaatttctga atgagttgat
     3181 ccacctgtgc cttacaataa atgaaatcag cgaatccgta cttatgctcg gggttcttac
     3241 gttggtggat attacgatgg ttgcaaacct gcttgtgatg gtgattatcg gcggatattc
     3301 gacgtttgtc agccgaatga atattgatga acatgaagat aaaccggaat ggctgcaaaa
     3361 agtagatgcc ggtacgctca aggtgaagct ggcgggatcg ctggttggtg tttcgggtat
     3421 tcaccttctt caggttttta ttaatataaa aaaccacaat tccgaacaag ttatgtggca
     3481 gacgattatt cacgtggttt tcctattttc ggcaatcatg ttggcgtact cagaaaaact
     3541 gttacacgag aagcacgggt agtcgatatt tttactttac ggcacaatgc gtttggccgt
     3601 aacatgatta ttaaatcgga tgtctttgat tattccgtca ttacccagtg tgataataga
     3661 ttggaatgaa aattccgaag aaccgttaaa tccgggttgt attatttcat aatttctttc
     3721 accgaatgcg ttatcgggtc tacgttttat tttgtaccac gtatccccgg tgatgcatct
     3781 ttttccccat tcttccgcat ttagttcgat ttttttagac gtcagtgaat ccgcttctcc
     3841 aacctccatg ggttttaact tcataaacaa catgtggtag ttagcaacaa tatccatatc
     3901 cagattgatg gaggtattgg ggccaccggt aatgcggtac gtgtt
//
LOCUS       DAOQGF010000198         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13001_length_3885_cov_5.713838, whole genome shotgun sequence.
ACCESSION   DAOQGF010000198 DAOQGF010000000
VERSION     DAOQGF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13001_length_3885_cov_5.713838"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PK937_15570"
     CDS             <1..385
                     /locus_tag="PK937_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34042.1"
                     /translation="SRGWFGDGNNLSFTGSDASDSLKQYRSGFSVRNMGDGYFPTKIL
                     VCFADGDSIVETWDGQYAPQEFVYHGKAKIKSVHIDPDRVNLLDINWTNNSRVLERDE
                     VGIQRYITRFLFWLQNIGQVLAVLV"
     gene            398..1246
                     /locus_tag="PK937_15575"
     CDS             398..1246
                     /locus_tag="PK937_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34043.1"
                     /translation="MKLTHIYFSAWHATWNNRRMAIMLWFTTLITAIPGYVMAYRTMG
                     QYFGRTEVPSLWKNGFELNYLFDMMIDQPSAGTIISSVFGGAAIVSLILMIFLTGGIV
                     AALIHPNEQNHFWHNCGKYFGRFFRLFLWNAVFVMIGIALIPTYEYGWIITVILSGTA
                     IMAGDIAKIRIVYNAHYSVTRMYFQSWRWVWQHVHHISELYIANVIAFVAVFALYKYL
                     DNGFVANNGLKLLMMIVLHQLFVYFRAMMRIQFLAAPVALWKNLHYTVVPPRVVSSQE
                     NILQTA"
     gene            1171..1644
                     /locus_tag="PK937_15580"
     CDS             1171..1644
                     /locus_tag="PK937_15580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNH34044.1"
                     /translation="MEKFALYRRAAAGRFVAGKYITNGVIGYRVKIKKSPIHGRGIFA
                     DEKIPARRKIGEMTGAIITVREARRRAKQRGAIALVEFSDGWAVDGFDHGNAFARINH
                     SCTPNAWMRVYGHHVEFYARRVIRVGEEITCDYVESHHDGKLRCRCGAKRCRGSI"
     gene            complement(1657..1977)
                     /locus_tag="PK937_15585"
     CDS             complement(1657..1977)
                     /locus_tag="PK937_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNH34045.1"
                     /translation="MKLGIIIETKEPEKAWNAFRFAVTSRKTGHEVKVFLMGEAVECE
                     GLTHEKYNVDEQLKKFIGEGGTILACGTCLKSRQLDGTESCPISTMTDCVQMVEWADK
                     VVTF"
     gene            complement(2169..2612)
                     /locus_tag="PK937_15590"
     CDS             complement(2169..2612)
                     /locus_tag="PK937_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34046.1"
                     /translation="MSDPIEQGLGPSFKLTGFVIDSFSLENEARQVENVDADMLLYHH
                     QGNAEIIPPKKLIRIKLSVTAEYEGKTLGKIVTQTEFEIINFEIFINNEILNIPGELM
                     SMLVGLAYSTTRGALIAKSEGTILAKFPMPVVSPSRLIGIAPMPS"
     gene            complement(2615..3040)
                     /locus_tag="PK937_15595"
     CDS             complement(2615..3040)
                     /locus_tag="PK937_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNH34047.1"
                     /translation="MSSVKNKFQELLKQVPDEVKLYVQMQGDIAIRVGEIIKEKQWTQ
                     KSLAEKMGWNESQVTRLLAGHENLTLKTIAKLSVVLEENLIQTQPRVVYIPLFPLDTP
                     ANAALNLGALRAASSVGNFFPLSTNKVTSFIKTEAKAVA"
     gene            complement(3043..3510)
                     /locus_tag="PK937_15600"
     CDS             complement(3043..3510)
                     /locus_tag="PK937_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34048.1"
                     /translation="MNPKYKIELLAEGPKAQIYTIRNLDSEISEFERFADEEDVRYDP
                     GFQEILARIDDMANRLGCIDSFFRNESARDNAICALFAPDGKLRLYCIKMRFTLLIIG
                     GGGIKNTRTYQEDPHLDSCVTLLEDFWKRFALYEDNGEIEISSDGKITGKDKI"
BASE COUNT          998 a          818 c          883 g         1186 t
ORIGIN      
        1 gtcccggggc tggtttggcg atggtaacaa tctgagtttt accggcagcg atgcatcgga
       61 ttcactaaaa caataccgaa gcggattttc cgtacgcaat atgggcgacg gttattttcc
      121 gacgaaaatt ttggtttgtt ttgccgacgg cgattctatt gtcgagacat gggacggtca
      181 atacgcgccc caggaatttg tttaccatgg taaggcgaaa atcaaatcgg tgcatattga
      241 tccggatcgc gtcaatttat tggatatcaa ctggaccaat aatagccgcg tactcgagcg
      301 ggacgaagtc ggaattcagc ggtatattac gcgttttctt ttttggttac aaaatatcgg
      361 ccaggtgctg gccgtattgg tttaaggaga atacacaatg aaacttacac atatctattt
      421 cagcgcttgg catgcaactt ggaacaaccg ccgtatggcg atcatgctgt ggtttactac
      481 tttgattacg gcgatacccg gatacgttat ggcgtaccgc acgatggggc agtacttcgg
      541 acgcacagag gtaccctcgc tgtggaaaaa tggttttgaa cttaactact tatttgacat
      601 gatgattgat cagccttcgg cgggtacgat tatttcgtcg gtctttggcg gagcggcgat
      661 cgtctcactt atactcatga tttttttgac aggcggcatt gttgcggcgc ttatccatcc
      721 caacgaacaa aatcattttt ggcataattg cggtaaatac ttcggtcgtt ttttccggtt
      781 attcttgtgg aatgcggttt ttgttatgat cggtatagcg ctcattccca cgtatgaata
      841 cggctggatt attacggtga tactctccgg tacggcgatc atggcgggag atattgctaa
      901 aattcgtatc gtctataatg cacactattc cgtaacgcgg atgtattttc agtcatggag
      961 atgggtttgg cagcatgtgc atcacatcag cgaactgtat atcgccaatg tgatcgcgtt
     1021 tgtggccgtt ttcgcactgt ataaatattt ggacaacgga ttcgtggcga ataacggact
     1081 aaaacttttg atgatgatcg tcttgcatca gctatttgta tatttccgcg caatgatgcg
     1141 gattcagttt ttggcggcac cggtagcgct gtggaaaaat ttgcattata ccgtcgtgcc
     1201 gccgcgggtc gtttcgtcgc aggaaaatat attacaaacg gcgtgatcgg ataccgagtc
     1261 aaaataaaaa aaagcccgat tcacggccgg ggtatttttg ccgatgaaaa aataccggca
     1321 cgtcgtaaga tcggtgaaat gaccggcgcg atcattacgg tacgtgaagc ccgtcggcgt
     1381 gctaaacaac gcggagctat tgctttggtc gaattttcgg acgggtgggc cgtagacggt
     1441 tttgatcacg gcaatgcctt tgcgcggatc aaccacagct gtacgcctaa tgcatggatg
     1501 cgtgtctacg gtcatcacgt cgagttttat gcgcgacgtg tgattcgcgt gggagaggag
     1561 atcacctgcg attacgtcga gtcgcatcac gacggcaaac ttcgctgccg atgcggtgca
     1621 aaacgatgcc gaggtagtat ttgaggattt ctatttttaa aacgtaacga ctttatccgc
     1681 ccactcgacc atctgaacac aatcggtcat cgtagaaatc ggacaacttt cagtaccatc
     1741 caattgccgt gatttgaggc atgtaccgca ggccaagatc gtaccgccct caccgataaa
     1801 ttttttcaat tgttcatcca cgttgtattt ttcatgcgtg agtccttcgc attcgacggc
     1861 ttcacccatc agaaatactt ttacctcgtg gccggttttg cgggatgtga cggcaaaacg
     1921 aaatgcattc cacgcttttt ccggttcttt ggtttctatg ataatgccga gtttcatgag
     1981 aattccttat ctgaagtggt gcggttaatc gtgttaggtt gtcttaccgc cgcttgattt
     2041 taagagtgga aaggtggagt taccggaata gtttgaattt gctccctcat ccgttctaat
     2101 ccgcgcaggg tacagtagaa caaagtgaac tgaagaaacc gtttttttga cagtacgctc
     2161 cggcggggtc atgacggcat cggtgcaatg ccgatgagac gagagggtga aacaaccggc
     2221 atgggaaatt ttgctaaaat cgtaccttcg ctttttgcga taagtgcgcc acgcgtagta
     2281 gagtatgcca acccgacgag catagacatg agctctcctg ggatatttaa aatttcgttg
     2341 ttgatgaaaa tttcaaaatt gatgatttca aattctgttt gcgtcacgat ttttcctaat
     2401 gttttccctt catactccgc cgttaccgaa agtttaattc ggataagctt tttgggcggt
     2461 atgatttctg catttccttg gtgatggtaa agcaacatgt cggcgtctac attttctact
     2521 tggcgcgctt cgttttccaa agaaaaagaa tctataacaa aaccggtcag tttgaaactt
     2581 ggtcccaaac cttgttcgat ggggtcagac ataattaggc taccgctttg gcttctgttt
     2641 tgataaaact ggttacttta ttggttgaaa gaggaaaaaa attgcctacg gatgatgcgg
     2701 cgcgaagggc gccaagattt agtgccgcat tggccggtgt atccaacggg aaaaggggaa
     2761 tgtacacgac ccttggttgc gtttgtatca aattctcttc caaaacaacc gatagtttgg
     2821 caatcgtctt tagcgtcaaa ttttcatgtc cggccaaaag gcgcgttact tgggactcgt
     2881 tccatcccat tttttcggcc agactctttt gagtccattg tttttctttg attatttcgc
     2941 ctacgcggat tgctatatct ccctgcattt gcacatagag cttcacttca tcgggaacct
     3001 gtttcaaaag ctcctgaaat ttatttttta cagaactcat atctatatct tatcctttcc
     3061 tgtgattttg ccgtcggagc taatttctat ttctccgttg tcctcgtata gtgcaaaacg
     3121 cttccaaaag tcttcaagta atgtaacgca agaatccaaa tgaggatcct cttggtaggt
     3181 tcgcgtattt tttatgccac cgcctcctat gatcagaaga gtaaaacgca ttttgataca
     3241 atataatcgt aattttccgt cgggtgcaaa aagtgcacaa atcgcattgt cacgtgcgct
     3301 ttcattacga aaaaaagagt caatgcaccc aagacgattg gccatatcat caatgcgtgc
     3361 taatatttcc tgaaaaccgg gatcataacg tacatcttct tcatcagcaa atctttcaaa
     3421 ctctgatatt tcagagtcca agttgcgaat cgtgtagatc tgtgcttttg ggccttcggc
     3481 taataattct attttatact ttggattcac ttataagtaa agttttttgc agatataatc
     3541 aaggcttttc agttacgaat caaacatttt attttcaatt gtttaactga ggtattcgta
     3601 gataccgcat acgtatattt ccaaagtctc ttctttgggt taccttatta atttaaaatc
     3661 agccgattca atcatatgat taaagtaata ggtatttttg aatgattttc attcaaattt
     3721 tgaccgaatt tttatcagaa ctgctttggt tgtatgtaag gcttacgtat atagataggc
     3781 gttatccgat atgaggactc cgcaaagaac gaacacagga aaactataac caatatttcc
     3841 taaaccatga tcgccggatt catccaaaaa caaaaaatat ttgat
//
LOCUS       DAOQGF010000199         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13060_length_3875_cov_7.823560, whole genome shotgun sequence.
ACCESSION   DAOQGF010000199 DAOQGF010000000
VERSION     DAOQGF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13060_length_3875_cov_7.823560"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2277
                     /locus_tag="PK937_15605"
     CDS             <1..2277
                     /locus_tag="PK937_15605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNH34049.1"
                     /translation="PKAKTNVKYEFLDIPEVRKSFIQFEHDHRVRVTLFLPQIHCSAC
                     IWLLEHLHKMESGIQSVRVDFLRKEAYILFDDRLTSLRQIAERLASIGYEPDFSRHDK
                     SGATAPAVERHLYIKLGVAGFCFGNIMLLALPDYLAGGQIEAPLRDYFRYLSIVVALP
                     LLYAVSGFFSSTFNALKEKTITMDVPISIGITTLIVRSLWDITHGFGSGYLDSLAGLT
                     FFLLIGKIFQKKTFYTLSFERNLKSYFPLAVQKKTDDSAVVIPIEQVQVGDLLIVRHQ
                     ELIVADSVLINGIATIDYSFVTGESIPVSVMQGDKIFAGGRQTGSAIEVEVVKEVSQS
                     YLQQLWNQEAFTGSSESRIQNISNTVAKYFMVAVLTIAVITGLYWYVTDPAMVIDTVT
                     SVLIVSCGCGLPLSIPFTFGTALRVLSEQQFYIKNYHVVENLAVINTIVFDKTGTLTK
                     SQGSSVSFEGSSLTKEQQAIIAAVVRNSTHPLSRAVYDYLDHVETYVVDRYEEISGKG
                     ITATVHGHHVKIGSLAWLEGLTLAENSPFYSDAQRSQMHIVIDGSLLGCFTILSEFRE
                     GTEYLSKVIPKDYQLFVLSGDSDREKNRLEQLFTNRAVMLFRQLPIDKLQFIRQQQVS
                     GRRVLMIGDGLNDAGALKQSDVGVAIAENTAAFTPSSDAIMDVKALPQLARFLIFSKQ
                     TKKTVYAAFGLTLLYNLVAVTLAVQGLLAPVAAAVIMPISAISVVAFTVIAVRWIARK
                     NQLLKTVSDVPIPSLSNA"
     gene            2274..2456
                     /gene="ccoS"
                     /locus_tag="PK937_15610"
     CDS             2274..2456
                     /gene="ccoS"
                     /locus_tag="PK937_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNH34050.1"
                     /translation="MNILVVLIAVSLMLAFGFLAAFLWAMNRGQYEDTHTPAMRMLLD
                     ENQNHITSNPSSSTEN"
     gene            2462..>3875
                     /gene="ccoN"
                     /locus_tag="PK937_15615"
     CDS             2462..>3875
                     /gene="ccoN"
                     /locus_tag="PK937_15615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNH34051.1"
                     /translation="MEKTSVAHEPQVTGRLEQFEYDNDIVRKFAIASVVFGIVGMLVG
                     VIIAIQIYMPALNLDIPWLTFGRIRPLHTNAVIFAFVGNGIFMGLYYSLQRLCKARLF
                     SDTLSNIHFWGWQLIIVSAAITLPLGITSGKEYAELEWPIDIAIALVWIVFGINMFGT
                     IIKRRERHMYVAIWFYIATWVTVTMLHVVNSLAIPAGWFKSYSVFAGVQDALVQWWYG
                     HNAVAFFLTTPYLGLMYYFMPKAVNRPVYSYRLSIIHFWALIFLYIWAGPHHLLYSAL
                     PDWAQSLGSVFSIMLIAPSWGGMINGLLTLRGVWDKVREEPVIKFMVVAVTAYGMATF
                     EGPMLSIKNVNALSHFTDWTIAHVHIGALGWNGFLTFGILYWLVPKMWNTTLYSKKLA
                     NAHFWLGFLGIVFYAVPMYWAGITQGLMWKQFTPDGTLQYFNFLETVTQIIPMYVIRS
                     IGGTMFFASMFIMAYNLIKTA"
BASE COUNT          999 a          783 c          934 g         1159 t
ORIGIN      
        1 cctaaagcca aaaccaacgt taaatatgaa tttttagata tacctgaggt acggaaatca
       61 tttatacagt ttgaacatga tcaccgcgtc cgcgtaacat tatttttacc gcaaattcat
      121 tgcagtgcat gcatttggct cttggaacat ttgcataaaa tggaatccgg tatacaatcg
      181 gtacgtgtag atttcctgcg taaggaagca tatatcctat ttgatgatcg gttaacctca
      241 ttgcggcaga tagcggaaag gcttgcttcg ataggttatg aacccgattt ttcaaggcac
      301 gataaatctg gggctacagc accggccgtc gagcgccatc tatatatcaa gttaggtgta
      361 gccggttttt gttttggtaa cattatgttg ctggcattac ccgactacct tgccggcggg
      421 cagatcgaag cgccactgag ggattatttt agatatttga gcatagtggt agcgctgccg
      481 ttactatatg cagtgagcgg ttttttttcg tcaacattta atgcgctaaa agaaaaaacg
      541 atcacgatgg atgtaccgat ttcgatcgga ataacgacgt tgatcgttcg cagtctttgg
      601 gatatcaccc acggattcgg ctccggttat ttggattcgc ttgcagggct tacttttttt
      661 cttttgatcg gaaaaatatt tcagaaaaaa acattttaca cgttgtcgtt tgaacgcaat
      721 ctgaaatcct attttccgct tgcggtacag aaaaaaacag acgattcagc cgtagtgata
      781 ccgatcgaac aagtgcaggt aggggattta ttgatagttc gtcatcagga acttatagtt
      841 gcggatagtg tattgatcaa tgggattgcg acgatagatt atagttttgt taccggggaa
      901 agtatacctg tatcggtgat gcagggtgat aaaatttttg ccggagggcg tcaaaccggc
      961 tccgctatcg aagtggaagt ggtcaaagaa gtatcacaaa gttatttgca acagttgtgg
     1021 aatcaagaag ccttcacggg ttcatcggaa agtcgaattc agaatatttc caatacagtg
     1081 gccaagtatt ttatggttgc ggtattaaca atcgctgtta ttacaggact gtactggtac
     1141 gtgaccgatc cggcaatggt gattgacaca gtgacttcgg tattgattgt ttcctgcggg
     1201 tgcggtttac ctctctccat tccctttaca tttggcaccg cattgcgcgt actgtcggag
     1261 cagcagtttt atattaaaaa ctatcatgtc gtagaaaacc tggcagtgat caatacgatc
     1321 gtttttgata aaacgggtac actgacgaag tcacaaggtt catcggtttc gtttgaggga
     1381 agttctttga caaaagagca gcaagctatc attgccgctg tagttcgtaa ctctacacac
     1441 ccgcttagcc gtgcggtata tgactatctg gatcacgttg aaacgtatgt cgttgatcgg
     1501 tatgaagaaa tatcaggtaa aggaattaca gcaaccgtac atggtcatca tgtgaaaatc
     1561 ggttctttgg catggcttga aggattaaca ttggcagaaa actctccatt ttacagtgat
     1621 gcacaacgta gccagatgca tatcgtgata gacggatcac ttttagggtg ttttacaatt
     1681 ttatcagaat ttcgtgaagg cacagaatac ctgagcaaag tcataccgaa ggactatcaa
     1741 cttttcgtac tctccggaga tagtgaccgt gaaaaaaatc ggcttgagca attgtttact
     1801 aaccgtgcgg taatgctttt taggcaatta ccgatagata aactacaatt tattcggcaa
     1861 caacaggtat ccgggcgtcg ggtattgatg ataggcgacg gactcaatga tgctggggcg
     1921 ctcaaacaga gtgatgtagg cgtagctatt gcagaaaaca cggccgcttt tacgccgagt
     1981 tcggatgcga tcatggatgt taaagcgttg cctcagttgg cgcgtttttt aattttttct
     2041 aagcaaacta aaaaaacagt gtacgcagcg ttcggactca ccctgttata taacttggtg
     2101 gctgttactt tggcggtgca aggtcttttg gcgccggtag ctgcagcggt gatcatgccg
     2161 atttcagcaa tctccgttgt agccttcacg gttatagccg ttcgatggat agcaagaaaa
     2221 aatcaacttt taaaaacggt atcggacgta ccgatacctt cattgagcaa cgcatgaata
     2281 tattagttgt acttatcgcc gtcagtctga tgctggcgtt tggtttttta gcagcatttt
     2341 tatgggccat gaaccgaggg cagtatgaag atacacacac acccgcgatg cgtatgctat
     2401 tagatgaaaa tcaaaatcac ataacctcaa acccttcatc atccacggag aattaaacgt
     2461 catggaaaag acatccgtag cgcatgaacc gcaggtgaca ggtcggctcg aacaatttga
     2521 gtacgacaat gacatcgtgc ggaagtttgc tattgcatcc gttgttttcg gtattgtcgg
     2581 tatgctggtc ggcgtgatca tcgccatcca aatctatatg ccggcgctca acctagatat
     2641 cccttggcta actttcgggc gcatccgacc gctacacacc aatgcggtta tatttgcgtt
     2701 tgtcggcaac gggattttta tgggtctcta ctattcgcta cagaggcttt gtaaagcacg
     2761 gcttttcagc gacacgttga gtaatattca tttttggggt tggcaattga tcattgtttc
     2821 ggcagcgatc accttaccgc tcggtattac cagcggaaaa gaatatgctg aattagaatg
     2881 gccgatagat attgccattg cgctcgtgtg gatcgtattt ggtatcaata tgttcggtac
     2941 gatcatcaaa cgccgtgaac gacacatgta cgtcgcgatt tggttttata tcgccacatg
     3001 ggtaacagtc actatgttgc acgtggtcaa ttcgttggcc attcctgcag gatggtttaa
     3061 aagttattcg gtgtttgccg gtgtgcaaga tgcgctggta cagtggtggt atggtcataa
     3121 tgccgtagcg tttttcctta cgacgccgta tctcggattg atgtattatt ttatgcccaa
     3181 ggcggttaat cgtcctgtgt attcttatcg tctttcgatc attcactttt gggcattgat
     3241 ctttttatac atctgggcag ggccgcacca tttgctttat agtgcgcttc cggattgggc
     3301 tcaatcgttg ggttctgttt tttcgatcat gctcattgca ccgagttggg gcggtatgat
     3361 caatggtctt cttacgttgc ggggcgtttg ggataaagtg cgtgaagaac cggtgatcaa
     3421 atttatggtg gttgccgtga ccgcgtatgg tatggcgact tttgaaggtc cgatgctttc
     3481 aataaaaaat gtcaatgcgc ttagccactt tacggattgg acgatcgcac acgtacatat
     3541 cggcgcactc ggatggaacg ggttcctgac tttcggtatt ttatactggc ttgtacctaa
     3601 gatgtggaat accacattat attctaaaaa attagccaac gcgcatttct ggctgggatt
     3661 tctcggtata gtgttttacg cggtgccaat gtattgggcc ggtatcacac aaggtctgat
     3721 gtggaaacaa ttcacaccgg acggcacctt acaatatttt aattttcttg aaacggtaac
     3781 tcagatcatc ccgatgtatg tgattcgttc catcggcggt acgatgtttt ttgcatccat
     3841 gtttatcatg gcttataacc tgatcaaaac ggcga
//
LOCUS       DAOQGF010000200         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13325_length_3826_cov_5.710952, whole genome shotgun sequence.
ACCESSION   DAOQGF010000200 DAOQGF010000000
VERSION     DAOQGF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13325_length_3826_cov_5.710952"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..506)
                     /locus_tag="PK937_15620"
     CDS             complement(261..506)
                     /locus_tag="PK937_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34052.1"
                     /translation="MIIFQFSDISTTGKIPENKDNLSDKDFKLLCCAHALSDWACIYA
                     GSHPPENFCNGMAAVGNFLGILATPDLLMKYADKNLT"
     gene            complement(506..1894)
                     /locus_tag="PK937_15625"
     CDS             complement(506..1894)
                     /locus_tag="PK937_15625"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNH34053.1"
                     /translation="MKPKNEQATGILIDAAIGTDIEHQYQSAMRTLLCACLRNPHQFH
                     QYAGRFSPVWWEETPYANLALAMFEQFNNGHRFSAASVLMGSGKEHNTDVLRRAMDSE
                     SDISLDFAFEHVEPTYQRWAEYRSAKAAIAAAADGAAADEIREAQDIRRRELDAFGHD
                     QSGEEVSQFDAWVKNKLDGIQEVFACRPPVLVLEDSGLLRGFAPGDLIVVAGRPSMGK
                     THFALNCANKFASDNLRGLFFSVEMTTDKLMRRLVGIRTGINPQSEWAILPDDKKREL
                     FEHAGQIKNSPCKIISGVTHINHLVSTAMAEHYLEPIRYILVDYLQLLQRTQTKNGNR
                     EQEISEMTYNIKQVGMSLKVPVILLAQLSRAVEQRADKRPQLSDLRESGAIEQTADIV
                     IFPHRPEYYGMEADEYGSLEGVAELIIRKNRNGKTGTVRCRFDGVTGFTDETPNPMPK
                     SSQFTRTENVPF"
     gene            complement(1898..2083)
                     /locus_tag="PK937_15630"
     CDS             complement(1898..2083)
                     /locus_tag="PK937_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34054.1"
                     /translation="MKNKQDYIMVLSPFAGIGSELYQAVKMNRRALGFELKKSYFDKA
                     CENLKSVQEQKQQLQMF"
     gene            complement(2080..2526)
                     /locus_tag="PK937_15635"
     CDS             complement(2080..2526)
                     /locus_tag="PK937_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34055.1"
                     /translation="MTPITNIEAVTDLLRRVEQYKPWSVSAMLDMPADARQGLAFTDN
                     DVRIVERCADLRIGPEWLVKYAHRVCAWMQNAVQVHQAEIQSSVSYTDAATIEVARMV
                     CSHLIGKHGEKEAMRRIIQEFQPDVEEGRLWVTAFRMAKKETNTTI"
     gene            complement(2599..3105)
                     /locus_tag="PK937_15640"
     CDS             complement(2599..3105)
                     /locus_tag="PK937_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34056.1"
                     /translation="MKFYFEGSFNPNPREEGKLAKVSIFPSTNSRACREIFVLVAKEI
                     PEVISGENYNLGLVGEKKTPMIFPPSNSKEDNRILVMGDIPQPKHRSDGYLDREKSTG
                     QLIDESMGGGAWGAGSCFLAILEDGQRMVSNRLIVWENKGGEMFKTQFNSLSEYELAY
                     ESPKVDFI"
     gene            complement(3266..>3826)
                     /locus_tag="PK937_15645"
     CDS             complement(3266..>3826)
                     /locus_tag="PK937_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34057.1"
                     /translation="ITRAGLFKMITRMEGKNLIEVDTQTRAIRVTSAFVDAENKVQPR
                     KQSLHGRVNKVDMARKQSLHGRVNKVDITNKVYKGDIKGEQGEIVSAAETSLQRLDRW
                     ISEISTGDEKRILLEAFQMVYKLPAARFDECLAAFRAKASTTAEKYLRRADVVEHFLN
                     FSNSHCKKNPARAASAKYFSAENRAY"
BASE COUNT          910 a          983 c          830 g         1103 t
ORIGIN      
        1 ctgtcgccca cctaccaccg gcgctatgcg cgccgatgcg gccggctttg agttacggta
       61 aagttgccct ttcgactcag cccacttaaa aggcgggaac catctaatcg ggcaacctat
      121 cccataacaa ggtataaaca aaatcggctg caacatctga acgagactaa ccgcactatt
      181 taaggtgatt tcaacctttc gggacattgc accgtttacc atgccgactt cgtttatacc
      241 cataccgtta aaaaataaaa tcaagtcaag tttttatcgg catatttcat caataaatcg
      301 ggagtagcca aaatgcctaa aaaattaccg acagccgcca taccattgca aaaattttct
      361 ggcgggtggc tcccggcata gatacatgcc caatcactca aagcgtgggc gcaacaaagc
      421 agtttgaagt ccttatcgga taggttatcc ttgttttcag gaattttacc tgttgtcgaa
      481 atatctgaaa attgaaaaat aatcattaaa aaggtacgtt ttcggttctc gtaaattgtg
      541 aggatttagg catcgggttc ggagtttcgt cggtgaatcc ggtaacgccg tcaaatcggc
      601 agcgtaccgt tccggtttta ccgttgcggt tttttctgat aatcaattca gcaacaccct
      661 ccaggctacc gtactcgtcg gcttccattc cgtaatattc tggccggtgt ggaaatatta
      721 ctatgtccgc tgtttgctcg atggctccac tctccctcaa atctgaaagt tgagggcgct
      781 tgtcggcgcg ctgctctaca gcccggctta gttgagcaag caggataacc ggcactttca
      841 gcgacattcc gacctgtttg atattgtatg tcatttctga aatttcctgc tcccggtttc
      901 cgtttttcgt ttgcgttctt tgtagcaatt gcaggtaatc taccaggata tatcggatag
      961 gctccaggta atgctcagcc atggcagtac tcaccaggtg gttaatatgt gtaacgcccg
     1021 aaattatttt gcatgggcta tttttgattt gcccggcgtg ttcgaacaat tctctttttt
     1081 tgtcgtccgg caatatcgcc cactcagact gaggattgat tcctgtgcgt atgccgacca
     1141 agcgccgcat caacttatcg gtggtcattt ccacgctgaa aaataggccg cgcagattgt
     1201 ccgatgcgaa tttatttgcg cagttgaggg caaagtgcgt tttacccata gaaggacgcc
     1261 cggcaaccac aataagatcg ccgggagcaa acccacgcag taaaccgcta tcctccagca
     1321 ccagaaccgg cggacggcac gcaaatacct cttgtatgcc gtctaatttg tttttcaccc
     1381 atgcgtcaaa ttgcgaaact tcttcgccgg attggtcatg gccgaaagcg tctaattccc
     1441 ggcgtcggat atcctgggct tctctgatct catcagcggc ggctccatcg gcagcggcgg
     1501 caatggcggc ttttgcggat cggtattctg cccatcgctg gtaggtaggt tcgacgtgtt
     1561 cgaaagcaaa gtcgagcgaa atgtcagatt ccgaatccat tgcccggcga agtacatcgg
     1621 tattatgttc ctttccggat cccatcaata ccgatgccgc actaaatcgg tggccattat
     1681 taaactgctc gaacatggcg agggccaaat tcgcataagg cgtttcttcc caccaaacgg
     1741 gagaaaacct cccggcatat tggtggaatt ggtgcgggtt gcgaaggcac gcgcatagca
     1801 gcgtgcgcat ggcgctttgg tattgatgct cgatgtctgt gcctatggcg gcgtcgatca
     1861 ggatgccggt tgcttgttcg tttttcggtt tcatggctta gaacatttgg agttgctgtt
     1921 tctgttcctg caccgattta aggttttcgc aagccttatc aaagtaggat tttttcaact
     1981 caaaacccaa agcgcggcgg ttcatcttta cggcctgata caactcagaa ccgatacctg
     2041 caaatgggct cagcaccatg atgtaatcct gtttgttttt catatcgttg tgtttgtttc
     2101 tttttttgcc atccgaaacg ccgtaaccca caatctccct tcttctacgt ccggctgaaa
     2161 ttcttgaatt attcggcgca tggcctcttt ttcgccgtgc tttccgatca aatgactgca
     2221 caccattcgc gctacttcaa tcgttgctgc atctgtatag gataccgagc tttgaatttc
     2281 tgcctggtgg acttgtacgg cgttttgcat ccacgcgcaa acccggtgcg cgtactttac
     2341 caaccattcc ggcccgattc gcaaatctgc gcagcgctca acgatcctaa cgtcgttatc
     2401 ggtgaacgcc aaaccttgcc ttgcgtctgc cggcatatca agcattgcag acacgctcca
     2461 cggcttgtat tgctccacgc ggcgcagcaa atcggtaact gcttcgatgt tagtgattgg
     2521 tgtcattttt ttataactgt cgcccaccta ccaccggcgc tatgcgcgcc gatgcggcgg
     2581 gctttgagtt acaggcaatc aaataaaatc aactttcgga gattcgtagg cgagttcata
     2641 ttcagaaagt gagttaaact gcgtcttaaa catctctcct cctttgttct cccaaactat
     2701 caatctattt gacaccatcc gttgcccatc ttccaatatt gcaagaaaac aagaacctgc
     2761 accccatgcg ccgccgccca tgctttcgtc aattaattgt cctgtgcttt tctccctgtc
     2821 caaataccca tctgaacggt gcttgggctg aggaatgtca cccatgacca aaatccgatt
     2881 atcctccttt gaatttgaag gggggaaaat cataggcgtt ttcttttccc cgactaatcc
     2941 cagattgtag ttttctcccg aaataacttc aggaatctct tttgctacaa gtacgaaaat
     3001 ctcccgacac gccctcgaat tggttgaagg gaaaatagac actttagcaa gtttgccttc
     3061 ttcacgagga tttgggttga atgacccttc aaaataaaat ttcatctttt aaattgttgt
     3121 gcgtttcagt ttcttaatca aaacgcatat tgaaatacct ctttgaaact gataaatgaa
     3181 aaagagatta aataaaaact gcctgtaaca tcgcatacaa cggtcatgcc cagcaaaagc
     3241 tggtcacgcc gtgtatgcgt gaccgttaat atgccctatt ttcagcactg aaatattttg
     3301 cgctcgcagc ccgcgcagga ttttttttgc aatggctgtt gctgaaattc agaaaatgct
     3361 ctaccacgtc tgcgcgcctt aaatactttt ccgccgtggt gctggctttt gcgcgaaacg
     3421 cggccaaaca ttcgtcaaac cgcgccgcgg gaagcttata caccatttgg aacgcctcta
     3481 acaaaatcct tttttcatcg ccggtactaa tttcggagat ccatctgtca aggcgttgga
     3541 gcgatgtttc agcggcggat actatctctc cctgttctcc cttaatatct cccttatata
     3601 ccttattagt aatgtcaact ttgtttacgc gcccatgtaa actttgttta cgcgccatgt
     3661 caactttgtt tacgcgccca tgtaaacttt gtttacgcgg ttgcacctta ttttcggcat
     3721 cgacaaaggc cgatgtaacc cttattgcgc gtgtttgagt gtcaacctca ataaggtttt
     3781 taccttccat ccttgttatc attttgaaca atccggcacg cgtaat
//
LOCUS       DAOQGF010000201         3791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13525_length_3791_cov_5.082173, whole genome shotgun sequence.
ACCESSION   DAOQGF010000201 DAOQGF010000000
VERSION     DAOQGF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13525_length_3791_cov_5.082173"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..164
                     /locus_tag="PK937_15650"
     tRNA            92..164
                     /locus_tag="PK937_15650"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Trp,seq:cca)
     gene            194..376
                     /gene="secE"
                     /locus_tag="PK937_15655"
     CDS             194..376
                     /gene="secE"
                     /locus_tag="PK937_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNH34058.1"
                     /translation="MNRIKEFFMSVQTEMKKVSWPTREELMGSTGVVMVLWLILSLYI
                     FTSDNVLQAIVKKLLL"
     gene            401..1000
                     /gene="nusG"
                     /locus_tag="PK937_15660"
     CDS             401..1000
                     /gene="nusG"
                     /locus_tag="PK937_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNH34059.1"
                     /translation="MENTEKTDSPEIVTKDAPRPENTRWYTLRVYSGQEAKVRDHIKS
                     ELEIAGLKDLVFRIMIPEETIVEMKNGKKKEKTRSFLPGYMIVEMVDDKRVQHVITNT
                     PGVVSFVGPKNMPQPLRPDEVDRMLGRMHEKQTTETIEIPYKVGDAVKIIDGPFADFN
                     GFIDEINYEKKKLKVMVSIFGRSTPVELDFLQVKTVTNS"
     gene            1026..1448
                     /gene="rplK"
                     /locus_tag="PK937_15665"
     CDS             1026..1448
                     /gene="rplK"
                     /locus_tag="PK937_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNH34060.1"
                     /translation="MKKVVAQIKLQISAGAANPQPPIGPALGQHGVNIMEFCKAFNAK
                     TQDQKGMIIPVIITVYSDRSFTFILKTPPAAILIKKAINLEKGSAQPNRNKVGKISRA
                     KLMEIAKIKLPDLNTKDIKSATSMMEGTAKSLGLDIID"
     gene            1540..2244
                     /gene="rplA"
                     /locus_tag="PK937_15670"
     CDS             1540..2244
                     /gene="rplA"
                     /locus_tag="PK937_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNH34061.1"
                     /translation="MKKHSKRFKAAVAKIEKGKEYSISDTFKLIKETATTKFTETVDV
                     AVNLGVDPKHADQALRGTVMLPHGNGRTVRVLVITKTKEKEAKEAGADLVGFDEYLQK
                     IKEGWTDVDVIVATPEAMIELGKLGKVLGPKGLMPNPKSGTVTPDVTKAVKEVKAGRV
                     EFRVDKQGNVHVGIGKSSFDAKQLEENFKSFMTTIIRMKPATAKGTYLKSVHVSTSMG
                     PSVKVSNTEVGGLTIH"
     gene            2279..2806
                     /gene="rplJ"
                     /locus_tag="PK937_15675"
     CDS             2279..2806
                     /gene="rplJ"
                     /locus_tag="PK937_15675"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNH34062.1"
                     /translation="MKKENKEQIIADLADKFQKASSLYLTDFTGINVIKMEQLRNKFR
                     ETNSEFKVVKNTLAKQALNKAGYTVDLSKYLQGPTGIAFGYDDAVAPAKVISEFLKEK
                     ENDNLKVKICIIEKEMYAASKIGDIAKMPNKKDMLAQLVGLFNSPMQNVVMVLNAPMQ
                     NLVGVLESLKNQKAA"
     gene            2900..3289
                     /gene="rplL"
                     /locus_tag="PK937_15680"
     CDS             2900..3289
                     /gene="rplL"
                     /locus_tag="PK937_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNH34063.1"
                     /translation="MAISRADVLEYLASANMMEIGELVKEIKSKFGVEIAAPVAVAAA
                     PGGAPGAGAAAAAEEKTEFTVKLTNAGDKKIQVIKVLREITNLGLKEAKDAVDATPAV
                     VKEGVSKDEAEKIKAKFAEVGATVEVA"
     gene            3534..>3791
                     /locus_tag="PK937_15685"
     CDS             3534..>3791
                     /locus_tag="PK937_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34064.1"
                     /translation="MSTKKVIERVNFAKNPSVVPMPDLLEIQIDSYKEFLQEDVPAEK
                     RTNTGLQNVFLSNFPVDDAKELFKLEFLKYYVERPKYDVREC"
BASE COUNT         1195 a          762 c          891 g          943 t
ORIGIN      
        1 gtggtcgtac cgtaggcgcc ggtgtagtaa ccaagatcct caagtaagaa gaaaaaatat
       61 ccctccggcg aagagtcgga ggggcttgtt taggggagtg gctcaattgg cagagcggcg
      121 gtctccaaaa ccgtaggttg caggttcaag tcctgtctcc cctgcgaaaa aatattcagg
      181 agttaaggaa caaatgaaca gaataaaaga attttttatg agcgtgcaga ccgaaatgaa
      241 aaaagtgtct tggcctacac gtgaagagct gatgggttct acgggagttg ttatggtcct
      301 ttggctcata ttgtcgttgt atatttttac atcggacaat gtgttgcaag cgatcgtaaa
      361 gaaactcctt ctgtaatcgg atttattaaa ggaatcgcaa gtggaaaaca cggaaaaaac
      421 ggattctcct gaaattgtga cgaaagatgc accgcgtcct gaaaatacac gttggtatac
      481 gttacgtgtg tactcaggtc aggaggccaa agttcgcgat catatcaaaa gtgaactcga
      541 gatagccggt cttaaagatc tcgtttttcg cattatgatt cccgaagaaa ccatcgtcga
      601 aatgaagaac ggtaaaaaga aagaaaaaac acgttccttt ttaccgggat atatgattgt
      661 tgaaatggtg gacgataaac gtgtgcaaca tgtaatcacc aacacacccg gcgttgtgag
      721 ttttgtagga cctaagaata tgccgcaacc gcttcgtccg gatgaagttg atcgtatgct
      781 cggtcgcatg catgaaaaac aaaccaccga aacgatcgaa ataccttata aagtgggtga
      841 tgcggtcaaa atcattgatg gcccttttgc ggatttcaac ggttttattg atgaaatcaa
      901 ctacgaaaag aaaaaactca aagtcatggt cagtattttt ggccgttcga caccggtgga
      961 actggatttt ctccaggtaa aaaccgtaac gaattcataa ttaaacacac aacggagaat
     1021 caactgtgaa aaaagttgtc gctcaaatta agttgcagat ttctgccggt gcagccaatc
     1081 cgcaaccgcc gatcggtccg gctctcggtc agcacggcgt gaacatcatg gaattttgta
     1141 aggcgtttaa cgccaagacc caggatcaga agggtatgat cattccggtg atcataacgg
     1201 tctattcgga tcgttcgttt acatttatcc tcaaaacacc tccggcagcc attttgatca
     1261 aaaaagcgat caatttggaa aaaggttcag ctcaaccgaa tcgtaacaaa gtaggcaaaa
     1321 tttctcgcgc taagctgatg gagatagcta aaatcaaatt gcccgacctc aatactaaag
     1381 atatcaagtc ggctacgagc atgatggaag gtaccgcaaa gagcttgggt ctggatatta
     1441 ttgattaatt tctaatttac tcataacaca ccacgtccgt gaggcatgtg ggagaaccag
     1501 tatgtgccgt atgcacagaa aattcgcaaa aggagaacta tgaaaaaaca cagcaaacgc
     1561 tttaaagcgg ctgtcgcaaa gatcgagaaa ggtaaggaat attctatttc cgatactttc
     1621 aagctgatca aagaaaccgc cacaactaaa tttacagaaa cggttgatgt ggctgtcaat
     1681 ctcggtgttg atccgaaaca cgccgatcaa gcgcttcgtg gaacggttat gttaccgcat
     1741 ggtaatggcc gtacggtgcg tgtccttgtg atcaccaaaa ccaaagaaaa agaagccaaa
     1801 gaagccggcg ccgatttggt tggttttgat gaatatctgc aaaaaatcaa agagggttgg
     1861 acggatgtag atgtgatcgt agctacaccc gaagctatga ttgagttggg taaattaggt
     1921 aaagtccttg gtcctaaggg cctcatgcct aatccgaaaa gcggaactgt cacaccggat
     1981 gttaccaagg cggtaaaaga agtgaaagcc ggtcgtgtgg aatttcgcgt tgataagcaa
     2041 ggtaatgtac acgttggtat cggcaaatcg tcgtttgatg ccaaacagct tgaagaaaat
     2101 ttcaagagct ttatgactac gattattcgt atgaagccgg ctacggcgaa aggtacatac
     2161 ctgaaaagtg tgcatgtttc cacgagtatg ggaccttcgg tcaaagtcag caacacggaa
     2221 gtgggcgggc tgacgataca ctaattaacg cgttgtcaaa cactaaacat ctacggccat
     2281 gaaaaaagaa aataaagaac agatcatagc agacctcgct gataaattcc agaaggcctc
     2341 cagcctctat ctgacggatt ttacgggtat caatgtgatc aaaatggaac agctccgtaa
     2401 taaatttcgt gaaaccaatt cggaatttaa agttgtcaaa aacacattag ccaaacaagc
     2461 gctcaataaa gccggctata cggttgatct gagcaagtat ttgcaaggac cgacgggtat
     2521 tgcatttggt tacgatgatg cggttgctcc ggcgaaagtt atatcggagt ttctcaaaga
     2581 aaaagaaaac gataatctca aagttaaaat ctgtattatc gaaaaagaaa tgtatgccgc
     2641 atcgaagatc ggtgacattg cgaaaatgcc taataagaaa gacatgctgg ctcagcttgt
     2701 cggccttttc aattcgccga tgcagaatgt ggtaatggta ctcaatgccc cgatgcaaaa
     2761 tctcgtgggt gtgctggagt ccttgaaaaa ccaaaaagca gcttaatttt tcattcattc
     2821 attaacgttt gcggccttaa tatgttgtcc gtgtaagtgt tgcggataac cggttgcaac
     2881 aaaagtcagg agtcctaaaa tggctatctc tcgcgcagat gtactggaat atcttgccag
     2941 tgcaaacatg atggaaatcg gcgaactggt caaagaaatc aagtccaagt tcggtgtcga
     3001 aattgcagcc cctgttgcag tagcagcagc tccgggcggc gctccgggtg ccggtgcagc
     3061 cgctgctgct gaagaaaaga ccgaatttac agtgaaactc accaacgccg gtgacaaaaa
     3121 aatccaggtg atcaaagtcc tccgcgaaat caccaatctc ggcctcaaag aagctaaaga
     3181 cgctgttgat gcaacgcccg ccgttgtcaa agaaggcgta tcgaaagatg aagccgaaaa
     3241 aatcaaagcg aaatttgctg aagtcggcgc taccgtcgaa gttgcttaat cgtttttgga
     3301 ttgataagga aggcagttgg aatccaactg ccttccaatg ttttttgaat ttttgaaaaa
     3361 tacgcggaga acgttaaaaa cgattttgcg ctctcggcgt gttttttagt tttgatccga
     3421 taaagaaatc caggcgataa tatgggtttc tcccgcaagc gaacatgcct ttcgcttttt
     3481 ctacaaaaaa attaagagtt ttctgacaac cttaattaaa aggcaggatt ccattgagta
     3541 caaaaaaagt tattgagcgt gtgaacttcg ccaaaaatcc gagcgtagtt cccatgccgg
     3601 atcttctgga aatccagatt gattcctata aagaattttt gcaggaagat gttcctgcag
     3661 aaaaacggac caataccggt cttcagaatg tatttttgag taattttccg gtagacgatg
     3721 caaaagaatt gttcaaactt gaatttctga agtattacgt cgaacgtcct aaatatgacg
     3781 tacgcgaatg c
//
LOCUS       DAOQGF010000202         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13654_length_3764_cov_6.379617, whole genome shotgun sequence.
ACCESSION   DAOQGF010000202 DAOQGF010000000
VERSION     DAOQGF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13654_length_3764_cov_6.379617"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..750)
                     /locus_tag="PK937_15690"
     CDS             complement(277..750)
                     /locus_tag="PK937_15690"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNH34065.1"
                     /translation="MQTEGRNAIHRQWLFLNKLPLGSWIFSYFVNRYIPYTGSISARV
                     LELKPGYARVLLRDRRKVRNHLRSIHAIALANLAEYTGNLALVCGLPEDARFIVKNIA
                     VDYQKKARGTLVGECQTEIVATAETRDISVRVDIRDQDGDTVATGLLLTRIGPKK"
     gene            890..2479
                     /locus_tag="PK937_15695"
     CDS             890..2479
                     /locus_tag="PK937_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNH34066.1"
                     /translation="MKKIDFLDWSLDAWFLALCLMLCLTCMAFSCSLNAQEKDTVILR
                     DSAVTEGDSVDTFNATVQNVVNETRRIVKRRAQAASRFLNERYIIVIDPGHGGTKYID
                     KGNSAKLDGIEYHEKDMMFEYAQLVEKKLAAAGYEGLRKTKEAADDSSLSIYTRAGRA
                     AQFGKNAGKKVLFVSLHWNNFEDPSVGGTEIYIKESQNYKSRKLAEFLRGSMSHILQM
                     HGAGQNITGIVERDYRVLREMDTGVLIEIGYASNPGDLRKMLSQKDEIATAIVNGIDA
                     FAEYMQEQEAQKQMPNDDPKKAAEDAKLAEIPSIPFGWSWRKKDISADVDHLMKRYDG
                     MYEKVTTEKEIYLTFDCGWENGYTPKILDVLKANEVKAAFFITGGYLKSAEDVVARMV
                     QEGHIVGNHSVNHLSMPQLKVREMKREILGLEQDFYDLFEMKMNYFRPPSGEWSRRSL
                     AVTQALGYKTVFWSFAYDDWDVQAQHGRDYAYKKITQNVRPGVIILLHAVSKDNFEAL
                     DTVIKDLRKDGYVFKSLNEIK"
     gene            2496..3236
                     /gene="ubiE"
                     /locus_tag="PK937_15700"
     CDS             2496..3236
                     /gene="ubiE"
                     /locus_tag="PK937_15700"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNH34067.1"
                     /translation="MGTHDPAADAVLPKPEEKKQYVRAMFDGVASKYDFLNHLLSLGI
                     DIYWRRRALKLINFSMNPKLLDLATGTGDVAIMAVRMGAQHVYGVDLSEGMLAYGRKK
                     IEKEKMTDAIDMICGEGERIPMEDACVDASTIAFGIRNVVDIPQTLREMARVTKTSGM
                     IVVLEFSQPQWPVMKQLYQFYFTQVLPRIGSLFSKDKKAYHYLPASVMRFPAREEFAH
                     MMEQAGYTNVRHFDMTLGIVTVYSGIKL"
     gene            3257..>3764
                     /locus_tag="PK937_15705"
     CDS             3257..>3764
                     /locus_tag="PK937_15705"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNH34068.1"
                     /translation="MLIEKRVTEFLDELASSVPAPGGGSVAALAAALGAALTAMVAQL
                     TIGKKKYADVEPIMQEISRKAETLRRDTTALIDKDTIAFNAVMTAFAMPKETDEQKQA
                     RASAIESATQNATRIPLDVMKLCASAIGLTHTVVTRGNTNALSDGGVGALMLQAACRG
                     AYYNVMINL"
BASE COUNT         1059 a          800 c          971 g          934 t
ORIGIN      
        1 gtctgaagcg cgcaggttac cagattttga gataatccat ctttgagcgt ataggatcgc
       61 acctggaaat ggcgttgtgg tttagcgtct ccgccttgga cggcgtaacc acaaaccatg
      121 atccatatgc acaataccgt tgcaaaacga cgccgtgggg acatttgaaa aaagattaat
      181 gaattgagat cggtgtagtg aaaactaatc gaaaagagtc gaaaaaaaaa gtgaaaaaat
      241 attatttaat ccgtgcctga tttgtaacgt caaaatctat tttttaggac cgatgcgcgt
      301 aagtaataaa ccggtggcta cggtgtctcc gtcttggtca cggatatcaa cccgtacgga
      361 gatgtcgcgc gtttcggctg tcgccacgat ctcggtttgg cattcgccaa caagtgtgcc
      421 gcgcgctttt ttttgataat ccaccgcgat attttttacg ataaaacgcg catcctccgg
      481 taatccgcat accaatgcaa ggtttcccgt atattcagcg aggttggcaa gagcgatggc
      541 gtgtatcgaa cgcagatggt tgcgcacttt gcgccgatcc cgtaataaaa cacgcgcata
      601 accgggtttg agttccaaaa cgcgggctga tatgctcccg gtgtaaggta tgtagcgatt
      661 gacaaaataa ctgaatatcc aggaacccaa tggaagttta tttaaaaata accactggcg
      721 gtgtatagcg ttgcgtcctt cagtttgcat aaaacattat cagggttttt atgaaaaata
      781 caatttcatt ccgttcatac gcttattgta aaggaaatcc gactaaattc caattgaatt
      841 gtgtctgtga aaagactact ttacgcgcga atttaaagaa aatgtttata tgaagaaaat
      901 tgatttttta gactggtcac ttgacgcttg gtttttagcg ttatgtttga tgttgtgttt
      961 aacatgtatg gccttcagtt gttcattgaa cgcacaggaa aaagacacgg ttattttgcg
     1021 cgactccgct gtaaccgagg gcgacagcgt ggacacgttt aatgccacag tacagaatgt
     1081 ggtgaatgaa actcgacgta tcgtcaaacg acgcgcccaa gccgcatcgc gttttctgaa
     1141 cgaacgctac atcatcgtca ttgacccagg ccatggcggc acaaagtaca tagacaaagg
     1201 gaattcggcc aaattggatg gcatcgaata ccacgaaaaa gacatgatgt ttgaatatgc
     1261 acagctcgtg gagaaaaaat tagctgcggc aggatatgaa ggactgcgaa aaacgaaaga
     1321 agcggcggat gattcgtcgc tatccattta cacgcgtgcc ggtcgtgcgg cccaattcgg
     1381 aaaaaatgcg ggcaaaaaag ttctttttgt cagcctgcac tggaataatt ttgaagatcc
     1441 ttcggtcggc ggtacggaga tatatatcaa agaaagtcaa aattacaaaa gccgtaaatt
     1501 ggcagaattt ctgcgcggtt cgatgagtca tattttacaa atgcatggcg ccggtcaaaa
     1561 tattacgggt atcgtcgaac gcgattatcg cgtgctgcgc gaaatggata ccggcgtttt
     1621 gatcgaaatc gggtacgcaa gcaaccccgg cgacctccga aagatgctgt cacaaaaaga
     1681 cgagattgcc accgctattg tcaatggcat tgatgctttt gcggaatata tgcaggaaca
     1741 agaggcgcaa aaacaaatgc ccaatgatga tcccaaaaaa gcggctgagg atgccaagtt
     1801 agccgaaatt cccagtattc cgtttggctg gtcctggcgc aaaaaagata ttagcgccga
     1861 tgtggatcat ctgatgaaac gctacgatgg gatgtacgaa aaagtcacta ctgaaaaaga
     1921 aatttatctg acgtttgact gcggttggga aaacggatat acgcccaaaa tattagacgt
     1981 gctgaaagcc aacgaagtca aggccgcgtt tttcattacc ggcggatacc tgaagtcggc
     2041 ggaggatgtc gtggcacgta tggttcagga aggtcatatc gtcggtaatc attccgttaa
     2101 ccacctcagc atgccacagc tcaaagtgcg cgagatgaaa cgcgagattc ttggtttaga
     2161 acaggatttt tatgatcttt ttgaaatgaa aatgaattat ttccgtccgc ccagcggcga
     2221 atggagtcgt cgttcgctgg cggtgaccca ggcgctggga tataagaccg tattttggtc
     2281 gtttgcgtac gatgattggg atgtacaggc acaacacggg cgggattatg cgtacaaaaa
     2341 aatcacccaa aatgttcgcc ccggcgtcat tatcctgttg catgcggtat caaaggataa
     2401 ttttgaagca ttagacactg tgatcaaaga tctgcgcaaa gacggctatg tatttaaaag
     2461 tttgaatgaa attaaataaa gaacgataag agcgaatggg tacacatgat ccagcggcgg
     2521 atgccgtact tcccaaaccg gaagaaaaaa aacaatacgt gcgcgccatg tttgacggcg
     2581 tggcgtcgaa gtatgacttt ctcaaccatt tattaagcct tggtattgat atttattggc
     2641 gccgtcgtgc cctgaaactg atcaattttt cgatgaatcc gaaattgctg gatttggcca
     2701 cgggcacggg cgatgtggcg atcatggctg ttcgtatggg tgcgcaacac gtgtacggtg
     2761 tggatttatc ggaagggatg ttggcctacg ggcgaaaaaa aatcgaaaaa gaaaaaatga
     2821 cggatgcgat cgatatgatc tgtggtgagg gcgaacgcat accgatggaa gatgcgtgtg
     2881 tggacgcttc gacgatcgct ttcggcattc gtaatgtcgt ggatatcccg caaacgctgc
     2941 gcgaaatggc gcgtgtgacc aaaacatcgg gcatgatcgt ggtgctcgag ttttcccaac
     3001 cgcaatggcc ggtgatgaag caattgtacc aattctattt tacgcaagtt cttccgcgca
     3061 tcggttctct cttttccaaa gataaaaagg cgtatcatta tttgccggcg tcggtgatgc
     3121 gtttcccggc acgtgaagag tttgctcaca tgatggaaca agccggctat accaacgttc
     3181 gtcattttga tatgacgctg ggtatcgtga cagtttattc cggcatcaaa ttgtaaacgt
     3241 tttttcggag aacactatgc tgatcgaaaa aagagtaaca gaatttttag atgaattggc
     3301 ctcgtcggtg cctgcgcccg gcggaggcag tgtagccgcg ttggccgcag ctttgggagc
     3361 ggcgctcacg gcgatggtag cgcagcttac gatcggaaaa aagaaatatg ccgatgtcga
     3421 acctatcatg caggagatca gccgtaaagc ggaaaccctg cgccgtgata cgacagccct
     3481 gattgataaa gacacgattg cattcaatgc cgtgatgacg gcatttgcca tgcccaaaga
     3541 aaccgacgaa caaaaacagg cgcgcgcgtc ggccatcgaa agtgcgacgc aaaacgcaac
     3601 gcgtattcct ctggatgtta tgaaattgtg cgcgtcggca atcgggttga cccatacggt
     3661 cgttacgcgc ggcaatacca acgctctcag cgacggcggt gtcggtgcac tgatgctgca
     3721 ggccgcttgc cgtggagctt attataatgt gatgatcaat ttgg
//
LOCUS       DAOQGF010000203         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_13893_length_3724_cov_5.900245, whole genome shotgun sequence.
ACCESSION   DAOQGF010000203 DAOQGF010000000
VERSION     DAOQGF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13893_length_3724_cov_5.900245"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2034
                     /gene="infB"
                     /locus_tag="PK937_15710"
                     /pseudo
     CDS             118..2034
                     /gene="infB"
                     /locus_tag="PK937_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
     gene            2048..2329
                     /locus_tag="PK937_15715"
     CDS             2048..2329
                     /locus_tag="PK937_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNH34069.1"
                     /translation="MIVGVFKLDLFIHASASLKEKRFVVKSIKDGVRNKFNVSIAETG
                     HLDKWQRAELGVSMVANEEHLIRNTMDAIIHFIEAKGQCEIVNRELTMY"
     gene            2343..2684
                     /gene="rbfA"
                     /locus_tag="PK937_15720"
     CDS             2343..2684
                     /gene="rbfA"
                     /locus_tag="PK937_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNH34070.1"
                     /translation="MNQRMHRVQELILREIGVIIQTEMYDPRAKLVTVTAVRVSSDMS
                     FAKVYFTVMAPPDEIKAISKALDHAKGFLRSKIAERIEIKHIPELKFYYDDTLANADR
                     LEQIFKQIKGE"
     gene            2691..3446
                     /gene="truB"
                     /locus_tag="PK937_15725"
     CDS             2691..3446
                     /gene="truB"
                     /locus_tag="PK937_15725"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNH34071.1"
                     /translation="MFPVIGPHDLKAGKFFNTDELRLFFDEGKVLLIDKPAGWTSFDV
                     VNKIRFACKAKRVGHAGTLDPFATGLLILCTGKATKTIDTFSDLDKVYEAEFVLGKTT
                     DTLDIEGRITSEAPVPDLNQSVLQEICNRSIGEIEQIPPQFAAIKINGVPAYKMARKG
                     KTVDIKPRRITIHAYHVLSYKENILRVMVQCSKGTYIRALARDLGNELGCGAFVKSLR
                     RLSIGAYFVENSSDVLNMAERIKQALATSTDKS"
     gene            3465..>3724
                     /locus_tag="PK937_15730"
     CDS             3465..>3724
                     /locus_tag="PK937_15730"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34072.1"
                     /translation="MQILQGFDAINPNRPSVFTMGTFDGVHRGHQAVIKKLVEDAQAL
                     KARSVILTFDPHPQAVVNPEKAKSIHILTTIHEKQDIFAKLGV"
BASE COUNT         1134 a          779 c          887 g          924 t
ORIGIN      
        1 tatatattgc aaaatagatt gttttgtgat aagctattac tattattaaa agattccatg
       61 aaaaaatata tatattaata ttaactatta tgttaatgat ccatatataa atagaagaag
      121 aaaaataaga tagacgaaaa agacgtcgaa gcggctatcc gtgaaacgat ggctaagatg
      181 gatgattcgc gcacggccgg cgaagaacgt caaaaacggc gtaagcaaaa acgcgaagaa
      241 agagaagcag aagcggcaga gtccgaagaa gaacgtaatg tgattcgtgt gcatgaatat
      301 ctttcggcgc acgaattagc cgatatgatg gatgttgccg taggcgaaat tattaaaacc
      361 tgtctccagc ttggattgat ggtttctatc aatcaacgcc ttgatatgga taccattacg
      421 ctcgtagcgg gtgaattcgg atacgatgtc gagcaagtca aagagttcga ggaaaatgaa
      481 attgaagagt tggaagagca ggaagatgta ccggaatcta cccgtgcgcc gatcgtaacg
      541 atcatgggtc acgttgatca cggtaaaaca tcgcttcttg actatatacg taaggcgaac
      601 gtcgtagctg gcgaagcagg cgggatcacc caacatatcg gtgcttacga agttacgctc
      661 ccttcaggcc gaaagatcac atttttggat acaccgggcc acgaagcctt taccgctatg
      721 cgtgcgcgtg gtgcacaggt tacggatatc gtcgtgatcg tcgtcgcggc tgatgataat
      781 gtcatgcccc aaacggtgga agcccttaat cacgcgcaag ccgcgggcgt accgatcatt
      841 atcgcaatta acaagattga taagcccgaa gccaactcta tgcgtattaa aacgcaactt
      901 tctgaaaaaa atattcttgt cgaagattgg ggaggtaaat accaatgtgt ggaaatctcc
      961 gctaaaaaag gtttacacgt agatacatta ttggagaaga ttctcctcga agcggatgtt
     1021 ttggaactca aagcgaaagt agaatgccgc gcgaaaggaa ccattgtgga atcacaattg
     1081 gacaaaggac gcggagcgac ggccacagcg ttggttgagt caggtactct acgcatcggc
     1141 gatccgttta tttgcggtat ttattacggt cgtgtgaaag ctatgtttga tgaacgcggt
     1201 aattcggtaa aagccgccgg tccttcaaca ccggtgcagc ttatcggctt tgacggttta
     1261 ccccaagccg gggataaatt ggttgttacc tccaacgaac gtattgcccg taacattgcc
     1321 acaaaacgtc atcagctcaa gcgcgagcag gaccatcgcc gcgttaaatt ccttaccctg
     1381 gatgaaatct ctaaacaagt gcgcgacggc aaagtatcgg atttggcgat cgtactcaaa
     1441 ggcgatgtgg atggttccgt cgaagcactc agtgattcga ttcaacgcct cagtacaccc
     1501 gaagtgaagg tcaatatcat tcaccgcggc gtcggcgtaa taaccgaatc ggatgtgttg
     1561 ctcgcatcgg cctcgggagc gatcatcatc ggtttccacg ttcgtcccaa tctcaatgcc
     1621 cgtaaactgg ccgagcgtga aaacatagat atacgctatt acaacattat ttacgactgt
     1681 atcgaagata tccgcaaagc gctcgaaggt atgcttgaac cggattatga aattaaaatc
     1741 acaggtatgg tcgaaatacg tgaaattttc aaaatatcca aggtgggtac ggttgccggc
     1801 tgttatgtgg tggacggaaa agtcaatcgt acacagccca ttcgtcttct gcgcaacggc
     1861 gaagttgtac acgagggcaa actttcatca ctcaagcgtt tcaaagacga tgcccgcgaa
     1921 gttgcgcagg gatttgagtg cggtcttacc attgacggat acaatgatat caaatctggt
     1981 gatgtcgtcg aatgctacga acgcgtagaa accaaacgaa cattatcggt atgagcaccc
     2041 cacgatcatg atcgtcggag tttttaaatt agatttattt atacacgcaa gcgcttcact
     2101 caaagagaag cgctttgtcg taaaaagcat aaaagacggc gttcgtaata aatttaacgt
     2161 cagtatagcg gagaccggtc acttagataa atggcaacgc gccgaattgg gtgtcagtat
     2221 ggtcgccaat gaagaacatt tgattcgtaa tacgatggat gcgatcatac attttatcga
     2281 ggccaaaggc caatgcgaaa tcgtaaaccg cgaactgacc atgtactaac aatgagatta
     2341 atatgaatca acgtatgcac cgcgttcagg aactgatatt gcgggaaata ggagttatca
     2401 tacaaacgga aatgtacgat ccacgagcca aattggtcac cgtgacggcc gtccgcgtat
     2461 ccagcgacat gagttttgct aaagtatatt tcacggttat ggcgccgcct gatgaaatca
     2521 aagccatcag taaagcactg gaccatgcca aaggtttttt gcgcagcaaa atcgcagaac
     2581 gtatcgaaat caaacacatc ccggaattaa aattttatta tgacgacacg ttagccaatg
     2641 ccgatcgctt agaacaaata tttaagcaga tcaaagggga ataaacgcgc gtgtttcctg
     2701 taatcggacc gcacgatctg aaggcgggaa aatttttcaa caccgacgaa cttcgtcttt
     2761 tttttgatga aggaaaagtc ctacttattg acaaaccggc cggttggaca tcctttgatg
     2821 ttgtcaacaa aattcgtttt gcatgcaaag ccaaacgggt ggggcatgca gggacattgg
     2881 atccttttgc taccggctta ctcatcttgt gcaccggcaa agcgacgaaa acaatagata
     2941 cattttccga tttggataaa gtgtacgaag ccgagtttgt tttgggaaaa actaccgata
     3001 cattggatat tgaaggacgc atcacgagcg aagcgccggt tccggacttg aatcaatcgg
     3061 tattgcagga aatttgcaat agaagtatcg gcgagataga acaaattcct ccacagtttg
     3121 cagccatcaa gatcaatggt gtgcctgcat acaaaatggc tcgtaaagga aaaactgtag
     3181 atatcaaacc gcgccggata actatacatg cataccacgt tttatcttac aaagaaaaca
     3241 tattgcgggt gatggtacag tgttccaaag ggacttatat ccgtgcattg gcacgcgact
     3301 tgggaaacga attagggtgc ggagcctttg tcaaatctct gcgtcgctta tccattgggg
     3361 catatttcgt agaaaatagt tcggatgtgc tgaatatggc tgagcgtata aaacaagcat
     3421 tagcgacatc caccgataaa tcataatttt ttattctaaa ttttatgcaa attttacaag
     3481 gttttgacgc gataaatccc aatagaccat cggtatttac tatgggcaca tttgacggtg
     3541 tgcatcgcgg tcatcaggca gttataaaaa aattagttga agatgcgcag gcgttgaagg
     3601 cgcgttcggt tattcttacg tttgatcctc atccccaagc cgtagttaat cctgaaaaag
     3661 cgaaatccat acatattctt actacgatac acgaaaagca agatatcttc gccaagctgg
     3721 gggt
//
LOCUS       DAOQGF010000204         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14216_length_3671_cov_6.166482, whole genome shotgun sequence.
ACCESSION   DAOQGF010000204 DAOQGF010000000
VERSION     DAOQGF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14216_length_3671_cov_6.166482"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK937_15735"
     CDS             complement(<1..525)
                     /locus_tag="PK937_15735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNH34073.1"
                     /translation="MSSISEQKHQRVVELENELRSENFKRPQYRYGMDLVKATGLTKG
                     RIIEFGGGRGEFSRLLKDHGFEVVFTDINQANVEFAKSIGFEGYVIDANSGMADQKAD
                     SYDGAVMLEVIEHVTQAELLLKETHRILKPGGFLVLSTPNPYFLWRRLSVLFGKPIDG
                     EGYHWRYFTVYSLES"
     gene            complement(540..1598)
                     /locus_tag="PK937_15740"
     CDS             complement(540..1598)
                     /locus_tag="PK937_15740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH34074.1"
                     /translation="MVQTYIIGKELSNRNWEVHYVAYNKSGMRGYMRHEGLHVHWIKA
                     RRYLPILSLPSVWMALIRINPDVLYQRGRDILTGLMALYGLMYGKRTLWASAGETGVE
                     RKKYASQKNRQKGNFIRRWVLQIEALINDTICNFGMHRTDQMIVQTAYQQQRLLVTYA
                     RSSTIIKSGHPLPPSVQRALPIKVLWISSIKPVKHVDLFLDLADMCKNEPIEFWIAGQ
                     IVHEETGHKMQERIAKMNNIRYLGAVPFEQSSALIASSHILVNTTEVGYEGLPNAFVQ
                     AWLAGTVTLSLHTDPDAVIMKNDLGYVNPELVQLKEKMLRFIIEHAVWLRQSNACRQY
                     AVENYSVVSIVNQIEKTL"
     gene            complement(1678..2406)
                     /locus_tag="PK937_15745"
     CDS             complement(1678..2406)
                     /locus_tag="PK937_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNH34075.1"
                     /translation="MRRLFYSAFYFCVIVLFFVLLLDKWLMPMYVDLGKDIAVPAVTG
                     LSSEMAQKKLSELGFSVVLKRRYDISVAPKTVIQQSPEPQALVKAGRQIHLVIADDNQ
                     TVIMPSLTLSTYRDAVFNLQSIGLAIGVLDSASSNEFPQGVILKQSIEPNTVVRLGQS
                     VDLTISLGNNITESKVPFLINLSLKDAKARISDSGLRLGKIERKFLPDLIPDTVVGQS
                     LDSSLVVTPLTIIDLTVSSTDSEQ"
     gene            2560..>3671
                     /locus_tag="PK937_15750"
     CDS             2560..>3671
                     /locus_tag="PK937_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH34076.1"
                     /translation="MKKQHHQILVVDDEELNVRFLSQFLIRKGFAAITAHSGEEALAL
                     VASESPDLVLLDAMMPQMDGFEVCRRLRANPETALLPVVMVTALQRPEDEAHALDAGA
                     DDFLPKPINYTELMSRIRSLMRIKDLHDELRTNQDALEEKHDQLLQLQHLRESLTQML
                     VHDLKNPLTGIMGCTELLTMLSENMTEKQISILKKLEDNTGTLLKMVTELLDVSRMEE
                     NKLVIRPEAVSVKEVFMHNIGELEYMIEKYQLVMDMHLSDDNAMVWADRDLLMRIIAN
                     LLFNAIKHSQKGGKLTLTCEWQAAEHRMCFAVTDTGEGIPVEYLEKIFEKFSQVDLKQ
                     RGLKSDRGLGLTFCKLAVEAHGGKIWAESELGKGST"
BASE COUNT         1023 a          809 c          857 g          982 t
ORIGIN      
        1 ggattccagt gaatacactg taaaatagcg ccagtggtag ccttcgccgt ctataggctt
       61 accgaaaagc acagaaagac gacgccatag gaagtatgga ttgggcgtgc ttaagacgag
      121 aaatccgccg ggtttcaaaa tgcgatgtgt ttctttgagt aacaattcgg cttgggtaac
      181 gtgttcgatg acttccaaca tgacggctcc gtcgtacgaa tcggcttttt gatccgccat
      241 gccgctgttg gcgtcaatga catatccttc aaatcctatg gattttgcaa attcgacatt
      301 tgcctgattg atatcggtaa aaacaacctc aaaaccgtga tctttaagta gtcggctgaa
      361 ttcaccacga ccgccgccaa actcaatgat acgaccttta gtcagtcccg tggctttcac
      421 cagatccata ccatatcgat actgtggtcg tttgaaattt tcagagcgca attcattttc
      481 taattcgaca acccgttgat gtttttgttc gctaatacta gacataaatt tttctttaat
      541 tataaagttt tctcaatttg attgacgatg gaaaccactg aatagttttc tacggcgtat
      601 tggcggcatg cattactttg gcggagccac accgcatgtt caataatgaa ccggagcatc
      661 ttttctttta attgtaccaa ttccggattg acgtagccta aatcattttt catgatcacg
      721 gcatccggat cggtatgcag gctcaatgtt acagttccgg caagccaagc ttgcacaaaa
      781 gcgttcggta atccttcata accgacctca gtcgtattga ccagtatatg cgatgacgcg
      841 ataagtgcgc tgctttgttc aaacggaaca gcgccaagat agcgaatgtt gttcatcttt
      901 gcaatgcgct cttgcatttt atgacctgtt tcttcatgca caatctgccc ggcaatccaa
      961 aattcgatgg gctcattttt acacatatca gccagatcta aaaacaaatc aacgtgtttg
     1021 accggtttga tgctgctaat ccacagtact tttataggca aggcacgttg aacagatgga
     1081 ggcaagggat ggccggattt gatgatcgtc gaggaacggg cataagtaac aagcagacgt
     1141 tgttgttgat aggctgtttg tacgatcatc tggtcagtgc ggtgcatgcc gaagttacag
     1201 atcgtatcgt tgatcagcgc ttcgatttgc aatacccaac gacgaataaa atttcccttt
     1261 tgacgatttt tttgtgacgc atatttttta cgctcaacac ccgtttcgcc ggcgcttgcc
     1321 cagagtgtgc gtttaccgta catcaggccg tacaaagcca taaggccggt caatatgtcg
     1381 cgccctcgtt gatacagtac gtccggattg atgcgaatta atgccatcca tacggacggt
     1441 aaactgagta tgggtaaata gcggcgtgct tttatccaat ggacatgtaa tccttcatgc
     1501 ctcatgtagc cacgcatgcc ggacttatta taggctacgt agtgtacttc ccaatttcga
     1561 tttgaaagct ccttgcctat gatataagtt tgaaccatgg gtccggtaat agttgccgcc
     1621 ggatcggcaa taaatccatc agttacgaaa caaaattttc ggagattttt tgggaattta
     1681 ttgttcacta tcggttgaac tcacggtaag atcaatgatt gtcagcggag tcacgacgag
     1741 cgacgaatcc agcgattgac cgacgaccgt atcaggtatc aaatcgggca aaaatttacg
     1801 ttcgattttt ccgagtcgca aaccggaatc ggatatccga gctttggcat ctttgagact
     1861 gaggttgatc aaaaaaggta ccttggattc ggttatatta tttccgaggc ttattgtaag
     1921 gtcgacagat tgcccgaggc gaacgacggt attgggttca atcgattgtt tgagtataac
     1981 tccctgagga aattcattgg acgaagccga atcgagtacg ccaatggcaa gaccgataga
     2041 ttgtaaatta aaaacagcat cacggtatgt ggataaagtc agcgaaggca tgatgaccgt
     2101 ttgattatca tctgcgataa ccaaatgaat ctgacgacct gcttttacca acgcttgcgg
     2161 ttccgggctt tgttgtatca ccgtcttagg ggctaccgag atatcataac ggcgctttag
     2221 taccaccgaa aaccctaatt cactaagttt tttttgtgcc atttccgaag atagccctgt
     2281 gacggccggt acggcaatat ctttgcccaa atccacgtac atgggcatca accatttatc
     2341 cagtaaaaga acaaaaaaaa gaacgatgac acaaaaataa aatgctgaat aaaataatcg
     2401 gcgcataggg tggtgtatca aacaaaaaaa ctggtgtatg aagaaatcaa atacgtgcgc
     2461 gcaatataaa cggaatacga cataggcgca agacgatttg ggcttacgta aatttatttg
     2521 attatcgtgt acgaacttag tacacttgtg tccggtctca tgaaaaaaca acatcaccaa
     2581 atattagtcg ttgacgatga ggaattaaac gttcggttcc taagtcaatt tctcattcgt
     2641 aaaggctttg cagcgattac agcgcactcc ggcgaagagg cgttggctct tgtcgcgtca
     2701 gaatctccgg atcttgtttt gcttgatgcc atgatgccgc aaatggacgg atttgaagtc
     2761 tgtcgccgct tgcgggccaa tccggaaacg gcattactgc cggtggtcat ggttacggcc
     2821 ttgcagcgcc cggaggatga agcgcacgca ctggacgccg gcgccgatga ttttttgccc
     2881 aaaccgatca actacaccga gctcatgtcg cgcatccgtt ccctgatgcg tatcaaagac
     2941 ttgcatgacg aactgcgtac caatcaagat gcacttgaag aaaaacacga ccagcttctg
     3001 caactgcagc acttgcgcga atcgctcacg caaatgctcg ttcatgatct caaaaacccg
     3061 ctaaccggga ttatgggatg taccgaattg ctcacgatgc tgagcgagaa catgaccgaa
     3121 aagcaaattt ctattctcaa aaaactggaa gataataccg gtacgttgct caaaatggtg
     3181 acggaacttt tggatgtgag ccggatggaa gagaataaac tggtgattcg tccggaagcc
     3241 gtgtccgtca aagaagtgtt tatgcacaat atcggcgaac tggaatatat gatcgaaaaa
     3301 taccagttgg ttatggatat gcacttgagt gacgataacg cgatggtgtg ggcggatcgt
     3361 gatttactga tgcgcatcat cgctaacctc ctttttaatg ccatcaaaca cagtcagaaa
     3421 ggcgggaaac tgacgctgac gtgcgaatgg caagccgcag agcatcgcat gtgttttgcg
     3481 gtgacggata ccggcgaagg gattcctgtc gaatatcttg agaagatttt tgaaaaattt
     3541 tcgcaagttg atctcaaaca acgcggactc aaatcggatc gcggcttagg attgacattt
     3601 tgtaaactcg ccgttgaagc gcacggcggt aaaatatggg ccgaaagcga attaggtaaa
     3661 ggcagcactt t
//
LOCUS       DAOQGF010000205         3639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14424_length_3639_cov_5.822824, whole genome shotgun sequence.
ACCESSION   DAOQGF010000205 DAOQGF010000000
VERSION     DAOQGF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14424_length_3639_cov_5.822824"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1403)
                     /locus_tag="PK937_15755"
     CDS             complement(111..1403)
                     /locus_tag="PK937_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNH34077.1"
                     /translation="MTKSAYTGLLILILFVMAVSWDGRREKMAPLSKSAAISIKRVST
                     GLSTVGADSANIGAWVEHRLEGSTNANALYQGFIAFHFDEMDTMLTVHSPAGRTNAGI
                     FSGDKPTFSKTGYTASLQTDLSEEHYTVSTQPIANFSKIKLTQILTAPKNNERIVYLD
                     FIFEFAPKDSLTDLRQYSNFRFLMGYDGDIGTTTGGMNNDSTGYYGGNQTGVAYIYDA
                     ALSLYSGVALHGKNVSDSAGNFGLWHQTVNTNEFSAAKLDTLMNEMMIHPSWSSTVQN
                     TDAMVYWTLNRGTIVPDDFVPYTDTIRFTLVNGLSKTTVIEASKGIHVPDVLPPDEPS
                     SPLPTTFVLFNNSPNPFNPSTTIRFYIPEPMFVRLTVYNLLGQKVRTLIARAVEPGIS
                     QVVWDARNDVGQTVAAGVYLYRMETPQGIITRRMLLLK"
     gene            complement(1473..1883)
                     /locus_tag="PK937_15760"
     CDS             complement(1473..1883)
                     /locus_tag="PK937_15760"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNH34078.1"
                     /translation="MNKNIKIIAGVLLVGGTLVFLAISGFDEGKSYYKFVDEVQAMGE
                     DAYTKKLKVHGNVVDGSIRKNGSQLEFVITRNGATMPVKYIGKDPVPDTFKDGCEAVI
                     DGRYKKDGTFEGTQIQAKCASKYETDYNDLKKGS"
     gene            complement(1952..3238)
                     /locus_tag="PK937_15765"
     CDS             complement(1952..3238)
                     /locus_tag="PK937_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNH34079.1"
                     /translation="MKLHTKIMTGLGIGLLAGIITNQIIGPEAANHPDLQWFITNITD
                     PVGQIFLRLLFMVVVPLVFASLALGVANLGNLETLGRIGVRTILYFVLLTAAAAVLGL
                     LLVQIFHPGKSFDPEMQKTLMAQYGSSTSEVIGKSKDITFSVMTFVDMLLPRNMMQAV
                     VTMQMLPIIVLALLFGVMMTRMKNNHMDSVKNLLEAVNEFAVGVVDLAMRFAPMAVPC
                     LVFSVSARFGVSVLKPLGFYVILVFAGYILHLFGTYSLVLRLMAGINPLRFFQAVKPV
                     MVTAFSTSSSNATLPTTIRVTEEELKISPNICGFVLPLGATTNMNGTALYEGLTVLFL
                     AEIFGVDLTLGQQAFVVVMTVLTAIGTAGIPGGSLPMIVLVLQSVNVPAEGIGIILGV
                     DRILDMGRTVLNVTGDITAAAYIAKTQGEPAQFNEA"
     gene            complement(3254..>3639)
                     /locus_tag="PK937_15770"
     CDS             complement(3254..>3639)
                     /locus_tag="PK937_15770"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNH34080.1"
                     /translation="TYLVIYFMVAFLGIALEPSLEGVGTHRQLGLPPCGFLTITGYPC
                     PSCGLTTSVSLGMHGHWLDALRVQPFGVYLFLLMSVIAVLSLLGIWKKIPFSKYIQSH
                     TAEKLQLITAFVFLISWIYKILIMN"
BASE COUNT         1093 a          866 c          746 g          934 t
ORIGIN      
        1 cgatgcgttg accatccacg tactgaaaaa cggccactgc actgactaaa aaaatcaata
       61 cggcggccag caaaaccgat aatttggttt gaattttcat gctaggaaat ttatttcagc
      121 agcagcatgc gacgggtaat aattccttgc ggcgtttcca ttctatacag atatacaccg
      181 gcggcgacgg tttgcccaac atcattacgg gcgtcccata cgacttggct tataccgggt
      241 tcgactgcac gggctatgag cgtgcgcact ttttgaccta ataaattata aaccgtgagg
      301 cgtacaaaca tcggttcagg aatataaaat cgtatcgttg tcgagggatt aaagggattc
      361 ggcgaattat tgaacaatac aaaagtagtc ggcaagggtg atgacggttc gtccggaggc
      421 aacacatccg gcacatgaat ccctttggag gcttcaatga cagtagtctt agataacccg
      481 ttgaccaacg taaaacgtat cgtatctgta tatggcacga agtcatccgg cacgatcgtt
      541 cctcggttca atgtccagta caccatcgca tcggtatttt gcacagtcga actccacgac
      601 ggatgtatca tcatttcatt catcaaagta tcgagcttgg ctgcggaaaa ttcattggta
      661 ttaaccgtct ggtgccataa accaaaattg cccgcgctat cggatacatt ttttccgtgc
      721 aatgcaaccc cgctatacaa agaaagcgcg gcatcgtaga tataagccac accggtttga
      781 ttaccgccat aataacccgt gctatcatta ttcataccgc cggtggtcgt accgatatcg
      841 ccatcatatc ccattagaaa acgaaaatta gaatattgac gcagatcggt tagtgaatct
      901 ttgggagcaa attcaaaaat aaaatcgaga taaacgatgc gttcgttatt tttgggcgcg
      961 gtgaggattt gagtaagttt tattttagaa aaattggcaa tgggctgtgt tgacaccgta
     1021 taatgttctt ccgaaaggtc cgtctgtaga gatgccgtat aacccgtctt gctaaaggta
     1081 ggtttatctc ccgaaaatat accggcattg gtccggcctg caggactgtg gacggtcagc
     1141 atcgtgtcca tttcgtcaaa gtgaaacgcg ataaacccct gatacaatgc attcgcatta
     1201 gtactacctt ccaaacggtg ttcaacccat gcgccgatat tggctgaatc cgcaccaacc
     1261 gtggacaaac ctgtacttac gcgtttaata ctgatcgccg ccgatttgga caacggcgcc
     1321 atcttttcgc gacgcccgtc ccaagacaca gccatcacaa acagaatcaa aatcaataat
     1381 cccgtgtacg ctgatttggt catcccgtat ccttgaaata aaaaaagatc attcgcgatg
     1441 aatgatcttt tgttgaaaaa aaattttacg aattaacttc cctttttgag atcgttgtaa
     1501 tccgtttcgt attttgatgc gcacttagct tgtatctgtg ttccttcaaa cgtgccgtct
     1561 ttcttgtagc gtccgtcaat cacggcttcg caaccgtcct tgaaagtatc cggaacagga
     1621 tcttttccga tatatttgac cggcatcgtt gcaccgttgc gagtaatgac gaattcgagt
     1681 tgtgaaccgt ttttgcgaat actgccatcc acgacattac cgtgaacttt cagttttttg
     1741 gtataggcat cttcgcccat tgcttggact tcgtccacaa atttataata cgattttcct
     1801 tcatcaaagc ccgagatcgc caaaaaaacc aacgtcccac cgactaataa aacaccggcg
     1861 atgattttga tatttttatt cataaaaaaa acctttctgt gtacgtattc gttacatcag
     1921 tataatactt catcttactc aaaaaagcaa cttaagcttc attaaattgc gccggttcgc
     1981 cttgtgtttt ggcaatatac gctgccgccg taatgtcgcc ggttacatta agcaccgtcc
     2041 gtcccatatc caatatacga tcaactccga gaattatacc gattccttcg gccgggacat
     2101 tgaccgattg taaaactaaa acaatcatcg gcaaagaacc accgggaatg cctgcggtgc
     2161 ctatcgcggt aagaaccgtc atcacaacaa cgaaggcttg ttgtccgagc gtcagatcca
     2221 cgccaaaaat ttcagctaaa aacaaaaccg tcaacccttc atacaacgcg gtgccgttca
     2281 tattagtggt tgcacccaga ggtaatacaa atccacaaat attgggtgaa attttaagtt
     2341 cttcttccgt tacgcgaatg gtggtaggta atgtcgcatt gcttgaactg gtcgaaaatg
     2401 ccgtgaccat cacaggctta accgcttgaa aaaaccgcaa aggattgatc cctgccatca
     2461 aacgaagcac taacgaataa gtgccaaata aatgtaggat gtatccggca aatacgagta
     2521 taacataaaa acccagcggt ttgaggacac tcacaccgaa tcgtgctgaa acgctaaaca
     2581 caaggcatgg cacggccatc ggagcaaatc gcatggctaa gtcaaccaca ccgacggcaa
     2641 attcgttgac agcctcgaga aggtttttaa cggaatccat gtgattgttt ttcattcgcg
     2701 tcatcattac gccaaaaagc aatgccaaaa cgataatcgg cagcatttgc attgtcacca
     2761 cggcttgcat catattacgc ggcaatagca tatccacaaa cgtcatcacc gaaaaggtaa
     2821 tgtctttgct tttgccgata acttcactgg tggatgagcc gtattgcgcc atcaatgttt
     2881 tttgcatctc tggatcaaaa cttttacccg ggtgaaaaat ctgaaccaga agtaaaccca
     2941 gcaccgccgc ggcggctgtc aataaaacaa aatacaaaat ggttcgtacg ccgattcgcc
     3001 ccaatgtttc caaattaccg agattggcta ctcccaaagc cagtgatgca aataccaacg
     3061 gaaccaccac cataaaaaga agacgtaaaa atatctgacc gaccggatcg gtgatattgg
     3121 tgataaacca ttgcaagtcg ggatgattgg cggcttcagg tccgatgatc tgattggtaa
     3181 taattcctgc aagtaacccg ataccaagtc cggtcatgat tttggtgtga agcttcatct
     3241 tcaagtcttt ctattagttc ataatcaaaa ttttataaat ccagcttatc aaaaaaacaa
     3301 aagctgtgat taattgcagc ttttccgcgg tatgagattg aatatatttt gaaaaaggaa
     3361 tttttttcca aatacctaga agagaaagaa cagcgatcac ggacatcaaa aggaataaat
     3421 aaacgccaaa tggttgtaca cgaagtgcat ccaaccaatg cccgtgcatg cccaaactca
     3481 cggacgtagt caacccgcac gaagggcaag gataaccggt gatagtcaga aatccacatg
     3541 gcggcaaccc caattggcga tgcgtgccga caccctcaag actgggctcc aaagcaatac
     3601 ctaaaaaagc caccataaaa taaatcacaa ggtatgtca
//
LOCUS       DAOQGF010000206         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14443_length_3637_cov_7.556672, whole genome shotgun sequence.
ACCESSION   DAOQGF010000206 DAOQGF010000000
VERSION     DAOQGF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14443_length_3637_cov_7.556672"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PK937_15775"
     CDS             <1..1606
                     /locus_tag="PK937_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNH34081.1"
                     /translation="IPLAAIGGVFALYLRGMPFSISAGIGFIALFGVAVLNGIVLISY
                     FNQLEKEGEADIITRVLKGTTSRLRPVIMTAAVASFGFLPMAISSSSGAEVQKPLATV
                     VIGGLLSSTLLTLIVLPVLYVLFARRKKVNLSPVMAAGMGFLALSFASPGFAQEDVRR
                     ITANEAVSIALESSPKVRKAILQADRQSVLTKSAFDFGKTEVFYESEETKSGVKGNSR
                     VGVRQEINFPTVYFQQYRLQNQNRALSELELKLVKAEVTRDVRIAYDEWVFAQEQLRL
                     VLWQDSLYGHFVKATEIRLNTGETSSIEAMAARAQYQEIKVLRVSLEASLLNAETALR
                     NTLGVSYHILPSETTLRRVPWTAGDSTASSGLYEAIFNQSIAVTQSQAGLEKQALLPD
                     ISLGYFRQRVAGMSGFHGVEAGLRIPLWFLPNAGRIQASNRQVGIAEQERNAAIRQRS
                     AEYESRLRNLKAVGGFLDYYENEGLSQSDALIQVADKNYKSGDIGYLEYLQLLQKAIA
                     IRAHYLEYLHQFNETVITLNYMTEGK"
     gene            1609..2820
                     /locus_tag="PK937_15780"
     CDS             1609..2820
                     /locus_tag="PK937_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNH34082.1"
                     /translation="MKSIFKTILPFTVSLVMIACSSKPEAVKEKEAAHEEHSNIVEVT
                     KAQYDGIGIVVQPLSKKNLKNVVKASGFLKVPPQNQAHVASFISGMVKTIHVKAGDYV
                     RQGQILASLEHPDVVKLQEDYVTSQSRLMFLEKEYERQKTLNQENAGIVKIFQQTESE
                     YLGVKARSASLKSQITMLSLNLEDISAGKFSSAFPLRSPIDGYIAHIDASIGMTAEPS
                     KSLFEIIDNSKIHVDLLVYEKDLFKVKIGQKVDFVLTNQDNRLITGVVFSMSKAFENE
                     TKSVAVHAEIKNDPTIDLIPGMFVTGFITVGDGGVEAVPEEAIVKSEGKEYIFVEKTE
                     SKGKSEKAEETKEEGHYHFTLLPVKTGTADLGYVQITPLTEIAKDSKIVAKGAFFLLS
                     KMKEGEGGDEH"
     gene            2955..>3637
                     /locus_tag="PK937_15785"
     CDS             2955..>3637
                     /locus_tag="PK937_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNH34083.1"
                     /translation="MKLPIRDRKFLVLLVAVLVVITLEVFSLAGIHVPMPYASFMFLT
                     FILAIGYKVLWNGLKALVKLKFSSINLLMSIAVVAAFYLGEYPEAAVVIVLYTLSERL
                     EEIGIKTSKSALDELVSKSPKTATLKDGNVDVPIDTIEIGSVIQIKPGDSIPLDGKIV
                     FGNTTVDEATITGEPIPKDKREGDIVFAGTTNKNGFIEVETTKVSTDTTFSKIIRLTF
                     EAQANKSDT"
BASE COUNT         1057 a          754 c          897 g          929 t
ORIGIN      
        1 tatcccgctt gcagctatcg gcggtgtgtt tgcactttat ctgcgtggta tgccctttag
       61 catttcggcg ggcatcggtt ttattgccct ttttggtgtt gccgttctta acggcatcgt
      121 tttaatcagt tattttaatc aactggaaaa ggaaggtgag gcggatatca tcacgcgtgt
      181 tctcaaagga acaacatcac ggctaaggcc cgtgatcatg acggcggccg tcgcctcatt
      241 tggctttctg ccgatggcga tttcatcttc gtccggagca gaggtccaaa aaccccttgc
      301 gacagtggtg atcggcggac tgttgtcatc gactttactc acgctgattg tactaccggt
      361 tctttacgtg ttatttgcac ggcgaaaaaa ggttaatctc agcccggtta tggcagcagg
      421 gatgggcttt ctggcgctat cgtttgcatc gccgggattt gcccaagagg atgtacgacg
      481 catcacagcg aatgaagccg taagcatagc cttagagagc agccccaaag tacgcaaggc
      541 gattttgcaa gcggatagac agagcgtatt gacgaaatct gcttttgatt tcggaaaaac
      601 agaagtcttt tacgaatctg aggaaaccaa atctggcgtc aaaggaaatt cgcgagttgg
      661 tgtgcgacag gaaattaatt ttccaacagt ttactttcag caatatcgct tacaaaacca
      721 aaaccgtgca ctgagcgaat tggagctcaa actcgtcaaa gcagaagtaa cccgggacgt
      781 acgaattgcc tatgacgaat gggtattcgc acaggagcaa ttgcgattgg tcttgtggca
      841 ggatagtctg tatgggcact ttgtgaaagc gactgaaatc cggctcaata ccggtgaaac
      901 atcctctatc gaggcaatgg cggcccgcgc acaataccag gaaatcaagg tgctgagggt
      961 ttcattggaa gcatcactcc tcaatgctga aacggcattg cgaaatacgc ttggcgtttc
     1021 ttatcatatc ttaccctcag aaaccacatt acggcgagta ccatggacgg caggtgacag
     1081 tactgcgtct agtggacttt acgaggccat attcaatcag tctatagctg tgacacaatc
     1141 tcaagccgga ttggaaaaac aagctttatt gccggatata agccttggct attttcggca
     1201 aagagtagca ggtatgtcgg gttttcacgg ggtggaagct ggtttgcgga ttccactatg
     1261 gtttctaccg aatgcaggac gtattcaggc gagcaatcgt caggtcggca ttgccgaaca
     1321 ggaacggaat gcagctatac gacaacgatc agccgaatat gagtcgcggc ttcgaaacct
     1381 caaggccgtc ggagggtttt tggactacta tgaaaacgag ggtctatcac aaagtgatgc
     1441 tttgattcaa gtcgcggata aaaattataa gtccggcgat ataggatacc ttgaatatct
     1501 ccaattgcta cagaaggcga ttgcaatccg cgcccattat ctcgagtatc tgcaccagtt
     1561 taatgaaacc gtcatcacgc tcaattatat gaccgaagga aaatagaaat gaaatccata
     1621 ttcaaaacaa ttcttccgtt tactgtatcc ctagtaatga tagcatgcag ttcaaagcca
     1681 gaagccgtta aagaaaaaga ggcggctcac gaggaacata gtaatatcgt cgaagtgaca
     1741 aaggcacaat atgatggaat tgggatagtg gtacagccgc tttccaaaaa gaatcttaag
     1801 aacgttgtca aggccagcgg gtttctcaag gtaccgcctc aaaaccaggc gcatgtcgct
     1861 tcattcatca gtggtatggt aaaaacgatt catgttaagg ccggcgacta tgtacgacaa
     1921 ggacagattc tggcaagtct ggaacatccg gacgttgtga aactacagga agactatgtc
     1981 acatcgcaaa gtcgcctgat gtttttggaa aaagaatacg agagacaaaa gacactcaac
     2041 caggagaacg cgggtatagt caaaatcttt caacaaactg aatcggaata tctcggcgtc
     2101 aaggcgcgaa gcgcatcgct taaaagtcag attacgatgc tctcgctcaa tttagaagat
     2161 atatcggccg ggaaattctc ctctgctttt cccttgcggt cgccgataga cggttatatt
     2221 gcacatatcg atgcttctat aggtatgacg gcagagcctt ccaaatcgct atttgagatt
     2281 atcgacaatt ctaaaattca tgtcgatttg cttgtatacg aaaaggatct attcaaagtg
     2341 aaaatcgggc aaaaggttga ttttgtactt accaatcaag ataaccgttt gatcacgggc
     2401 gtagttttct cgatgagcaa agcttttgag aatgaaacca aatctgtggc ggttcacgcg
     2461 gaaatcaaaa acgatccgac gattgattta ataccgggga tgtttgtaac agggttcatt
     2521 acagtgggcg acgggggagt tgaagctgtg cctgaggaag cgatcgtaaa gtcagaaggg
     2581 aaagaataca tctttgttga aaagacagag tctaaaggta aatctgagaa ggccgaagag
     2641 actaaggaag aaggacacta tcattttacg ctcctgcctg taaagactgg aacagctgac
     2701 ctgggttacg tacaaataac accgttgact gaaattgcga aggattctaa aatcgtggcc
     2761 aaaggtgcct ttttcctgtt atcgaaaatg aaagagggtg aaggcggaga tgagcattga
     2821 gttggcaaac attattctca tcaaggggcc gggctattcc gaacgtaatc gcgccaatga
     2881 tgtgacaaca gtaccttgta tgtaatggtg atgctcacca atgcgagtgg agaataaacc
     2941 ataaaggcaa taaaatgaaa ttaccaattc gcgataggaa gtttctcgtc cttctggtag
     3001 ctgtgttggt ggttataact ttggaggtat tctcccttgc gggcatacat gttccaatgc
     3061 cttatgcatc gtttatgttt ttaacattca ttctcgcgat tggttacaaa gtcctctgga
     3121 atggattaaa agcactggtc aaactaaaat tcagcagcat taatctactg atgtccattg
     3181 ctgtggttgc ggcgttttac cttggcgagt accctgaagc ggctgtggtc attgttctgt
     3241 acacgttaag cgaacgtttg gaagaaatag ggattaagac cagtaagtca gctttggatg
     3301 aattagtttc caaatcgcca aaaacagcta cgctgaaaga tgggaatgtg gatgtgccga
     3361 tcgataccat tgaaatcggc tctgtaattc agataaaacc cggcgattct atcccgctcg
     3421 atggaaaaat cgtattcgga aacacgactg tcgatgaagc caccattacg ggcgagccta
     3481 ttcctaaaga caagcgggaa ggcgacatag tgtttgcggg aaccacgaat aaaaatggtt
     3541 ttattgaggt tgaaactaca aaagtttcaa cagatacgac tttttcaaaa attatccggc
     3601 tgacgtttga agcgcaggcc aataaatcgg atacgca
//
LOCUS       DAOQGF010000207         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14590_length_3615_cov_8.110955, whole genome shotgun sequence.
ACCESSION   DAOQGF010000207 DAOQGF010000000
VERSION     DAOQGF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14590_length_3615_cov_8.110955"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1777)
                     /locus_tag="PK937_15790"
     CDS             complement(<1..1777)
                     /locus_tag="PK937_15790"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNH34084.1"
                     /translation="MASKISTIRVGRILAVEREEYFKTGILFAYLFFMTAATIVGRTA
                     ADTLFLSRFDATKLSYMYLAISIALSVCGVAFHAIVDRFRRDRLIYAVTGGMILAVGV
                     TRIFVDSGWSLMYPMIYVGFEVFNFLLILQFWTFTNDVLDTRQAKRLIGIILSAGILG
                     GMISGFSIKALVHAIGTSNLITMYGGLMGLCVILVRLLARREFTDTVRETHSVTDKKA
                     HKKAEEKQKLGSIYHLKLIAIIVSIITMVLMFIDYQFKMVLKEYYRNDELAAVLGQFY
                     GYTGIVALVFQFFLSSRILSKYGILVSLLILPIFLLISSLSIWFAPAIPGTVMIGSLA
                     LYLMPILNAVVFAKASDKIFSDTIYSSASQLMYVPLHANVRGRAKVFVDGIIKPLSKS
                     GSAILLILSAHFFPVAVNDISLAVVGLLIICIIAIFLVKREYVKSLFSTLKANQIDFK
                     DINFNVSDSSAIEILVQGLESQDDRRVLYCLDALKNIEGFYLIPHVLTLMHHKSPRVR
                     TEALRVIEKITPRQATGEVIKRLEDDHTEVKVQAILTLSAYADDNHIKRILSFLIDED
                     SEIKGAAISGLMQYYGIDGIFYSVDI"
     gene            1957..3438
                     /gene="guaB"
                     /locus_tag="PK937_15795"
     CDS             1957..3438
                     /gene="guaB"
                     /locus_tag="PK937_15795"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNH34085.1"
                     /translation="MSLNFENKLVGEGLTFDDVLIVPMYSETLPNLVKVNTRLTKTIT
                     LNIPLISAAMDTVTESELAIAMAREGGIGIVHKNCSIEYQAAEVDRVKRSESGMIMKP
                     ITLTPEHKLSDALDLMKRYKISGIPITYADGKLAGILTNRDLRFATNYQNPIDTVMTK
                     NNLVTAKVGTTLDEAELILHKHRIEKLLVVDDSYILQGLITVKDIQKKKMYPHACKDK
                     HGRLRVGGALGVTKDTVERAQALLDAGVDVLGVDTAHGHSRGVLEMVKTLKQKFPQIE
                     IIAGNVATAEATEALIKAGADCVKVGIGPGSICTTRVVAGVGVPQITAILECARVAAK
                     AGIPIIADGGIKQTGDIAKAIVAGADTVMIGSLFAGTEESPGESVLYEGRQYKVYRGM
                     GSLSAMKQGSKDRYFQDDEMDTNKLVPEGIEGRVPYKGRLSDVVYQMVGGLRAAMGYC
                     GAESIETMKSKTRLMKMTGAGLKESHPHDIFVTKESPNYRMNS"
BASE COUNT         1047 a          877 c          863 g          828 t
ORIGIN      
        1 agatatccac ggaataaaat ataccgtcaa taccgtaata ctgcattaac cctgaaatgg
       61 ccgccccttt gatctccgaa tcttcgtcaa tcaaaaacga taatatgcgt ttgatgtggt
      121 tgtcatccgc atacgccgaa agcgtgagga tagcctgcac tttcacttcg gtgtgatcat
      181 cttcaagacg tttgatgacc tcgcccgttg cctgccgcgg tgtgattttt tcgatgacgc
      241 gcagagcttc ggtgcgcacg cgcggtgact tgtgatgcat caatgtcaac acatgcggga
      301 tgagataaaa tccttcgata ttttttagcg cgtctaaaca atacaatacg cgacggtcgt
      361 cttgactttc caggccctgc acaagtatct cgatggccga actgtccgac acgttaaaat
      421 tgatatcttt gaaatcaatc tggttggctt tgagcgtaga gaaaagcgat ttgacgtatt
      481 cacgcttaac gaggaaaata gcgatgatac agatgatcag taacccgact acggccaggc
      541 tgatatcgtt tacggcgacc ggaaaaaaat gggccgacaa aatcagcagt atcgcgcttc
      601 cgctcttgga taatggtttg atgatcccgt caacaaaaac tttcgcacgc ccgcgcacgt
      661 tggcatgcag cggtacatac atcagttggc tggccgacga atagatcgta tcgctgaaaa
      721 ttttatcgct cgctttcgca aaaacgaccg cattgagtat cggcatgaga tacagcgcca
      781 atgaaccgat catgaccgtg ccgggaatcg ccggcgcaaa ccaaatactg agcgaactga
      841 tcagcaaaaa aatcggcaga atcaaaagcg aaacaagaat accgtatttt gataaaatgc
      901 gcgaactcag aaaaaactga aataccagag cgacgatacc cgtgtagccg taaaactgac
      961 ctaacacagc cgccaattca tcattacgat agtactcttt gagcaccatt ttaaactggt
     1021 aatctataaa cataagcacc atcgtgatga tacttacgat gatggcgatc agtttgaggt
     1081 ggtagatact gccgagtttc tgcttttctt cggctttttt atgcgctttt ttatcggtca
     1141 cactgtgggt ttcgcgtacc gtatcggtaa attcgcggcg cgcaagcagg cgaaccaaaa
     1201 taacgcacaa ccccatcaag ccgccgtaca tcgtgatgag attggacgta ccgatggcat
     1261 gcaccaaagc tttgatcgaa aaaccgctga tcataccgcc caaaataccg gcgctcagaa
     1321 taatgccgat cagtcgtttg gcctgccgcg tatccaaaac gtcattggta aatgtccaaa
     1381 actggagtat gaggaggaag ttaaatactt caaatcccac atagatcatg ggatacatca
     1441 gcgaccaacc ggaatccaca aaaatacgtg taaccccgac ggccaatatc ataccgccgg
     1501 taacggcata gatcagccgg tcgcgtcgga agcgatccac tatggcgtga aacgccacgc
     1561 cgcatacgct gagcgcaatg gagatggcaa gatacatgta cgacagcttg gtcgcatcaa
     1621 aacggctaag aaaaagcgtg tccgcagccg ttcgtccgac gatggtagcc gccgtcataa
     1681 aaaaaagata tgcgaagaga atccccgtct taaagtattc ctcacgttct acggcaagaa
     1741 tacgtccaac gcgaatcgtc gagattttag aagccataca ccgtgtttgg caagacctga
     1801 ttccagtaaa taactagcgg acacaacagt atgtaatgta cataactttt aacgatagat
     1861 gcaagctgcg tacatgacac aaaaaagccc tctctgactt gcgtttgtag gtttagccga
     1921 ctatattgcg caactttttt tagcaaggag cttgttatgt cgctcaattt tgaaaataag
     1981 cttgtcggcg aaggcctcac gtttgatgat gtcctgatcg tgccgatgta ttccgaaaca
     2041 ttgcccaatt tggtcaaagt gaatacccgc ctaaccaaaa ccattacttt aaatattccc
     2101 ctgatcagcg ccgcgatgga tacggtcacg gaatcggaat tagccatagc tatggcccgc
     2161 gaaggcggca taggcatcgt acacaaaaac tgctccatcg aatatcaggc agccgaagtc
     2221 gatcgcgtca aacgttccga aagcggaatg atcatgaaac ccatcacgct gacaccggaa
     2281 cacaaacttt cggatgcgtt ggatttgatg aagcgttata aaatcagcgg tattcccatc
     2341 acgtacgccg acggcaaatt agccggtatt ctgaccaatc gcgatctgcg tttcgccacc
     2401 aactaccaga acccgattga caccgtgatg accaaaaata atctggtcac cgccaaagtc
     2461 ggcacgacac tggatgaagc ggagttgata ctgcacaaac accgcatcga aaaacttctt
     2521 gtcgtggatg atagttatat tttgcagggg ctcatcaccg tcaaagatat tcaaaaaaag
     2581 aaaatgtatc cccacgcatg caaagataaa cacggccgtc tgcgggtagg cggcgccttg
     2641 ggcgttacca aagatacggt ggaacgcgca caggctctat tggatgccgg cgtggatgta
     2701 ttgggtgtag atacggcgca cgggcactcg cgcggcgtgc tggaaatggt caaaacatta
     2761 aaacaaaaat ttccgcagat cgaaattatc gcaggcaatg tcgcaacggc tgaagcaacc
     2821 gaagctttga tcaaagccgg cgcggattgc gtcaaagtcg gcatcggtcc cggttccatc
     2881 tgtaccacgc gcgtcgtcgc cggtgtcggc gtaccgcaaa tcacggctat cctcgaatgt
     2941 gcacgcgtcg ccgccaaagc gggaatcccc atcatcgccg atggcggcat caaacaaacc
     3001 ggcgatatcg ctaaagcgat cgtggccggc gccgatacgg taatgatcgg aagtcttttc
     3061 gcaggcacgg aagaaagccc gggtgaatcc gttttgtatg aaggccgtca atataaggtt
     3121 tatcgcggta tgggttcgct ttcggcgatg aagcaaggca gcaaagatcg ttattttcag
     3181 gatgacgaaa tggataccaa taaactcgta ccggaaggta tcgaagggcg cgtgccgtat
     3241 aaaggccgct tgagcgatgt ggtgtaccaa atggtcggcg gcttaagagc ggctatggga
     3301 tactgcggcg cggaatccat cgaaacgatg aaaagcaaaa cccggttgat gaaaatgacc
     3361 ggtgccggac tcaaagaaag ccatccgcac gatatttttg tgacgaaaga atcaccgaat
     3421 tatcggatga atagttaaaa taccatcaca cccatcggtg tgggggcaat catcgtttat
     3481 catatctacg aataatcaga atttctcttc cggcgtttca ttttgatgaa gcgccgtata
     3541 taaaatatca tcctgcctta aattagataa aaaccgtata gaagctgtta tgtttttctt
     3601 tcgtgatgaa agccg
//
LOCUS       DAOQGF010000208         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14707_length_3598_cov_7.157211, whole genome shotgun sequence.
ACCESSION   DAOQGF010000208 DAOQGF010000000
VERSION     DAOQGF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14707_length_3598_cov_7.157211"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="PK937_15800"
     CDS             complement(<1..1678)
                     /locus_tag="PK937_15800"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH34086.1"
                     /translation="MKLLLLIGIGLNLSSTFSIVALSQTHAFTNVHVIPMDSFRILHH
                     QTVIITDGKIAAIGNTREVSIPKEASIIDGKGQYLIPGLIDCYAHIHEKNLMLFLANG
                     ITTVRNVPGAAFQHAIKKLSDTGKLQGPRIYSTGPAVTGSVVAYHTQGMLLNADEARF
                     AVRDVKRMGYQSVFSYVTITPDIYSAVLDEARQQGLPVQGHVPYMIPFEEYAEGSQLS
                     FDNLVGLLNPRSGDTYPKEQLIQFAEAFRKNGKYVIPTLTIHKARSLAHKSDSLKLRA
                     EMDYVPPRQRVYWHLNSLNYRYSGASEVVKVFYENGVKLLIGSDAGFHFAIHGFSYLE
                     EVQNFAELGIPHYDILKSGTSSAAEFLGWDDKIGTVSIGKEADLLLVPENPLEKITTL
                     AHHNGVMSRGVWYSRSKLNESLRSLKNELRDMPGSDRFSEFSAPDKNYHLLAHYAISY
                     KDIRCGDEKIFSSINSKNQMRLLSQNSIDPPCQRNTTTTWLIDNENILFTDVHRTSIE
                     GSTQVTMTHQFPSSTRVHGNHPVYGEFDYVDSLNTYRITGGPNTSINLDMD"
     gene            complement(1710..2336)
                     /locus_tag="PK937_15805"
     CDS             complement(1710..2336)
                     /locus_tag="PK937_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNH34087.1"
                     /translation="MHVLIMITCISLGSFSFGSCQDGEATDSTVVVADTSDFVLPFPV
                     GKRYKVSQSNNTIWSHSPNNFNGVFRYAYDFDMPVGTVLTASREGEVVFISDNFEDSD
                     HTLGHENVLVIRHRDATYSRYIHIRKHGALVKTGATVAKGDTVALSGNSGYTLGIPHL
                     HFDVVTAPTGLPDINAQSIRTLFKNSTAYPNALQQNERYRALPYTFSK"
     gene            complement(2348..2632)
                     /locus_tag="PK937_15810"
     CDS             complement(2348..2632)
                     /locus_tag="PK937_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34088.1"
                     /translation="MKKVSTILTRHPQGKQGVRIAKSKYDVIRGFIVLTLKERGPMPF
                     EELTDHAVRTLSSDFEGSVVWYIVTVKLDLEARKLIIRLPRKSPQWISLS"
     gene            3001..>3598
                     /locus_tag="PK937_15815"
     CDS             3001..>3598
                     /locus_tag="PK937_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNH34089.1"
                     /translation="MIKFTINGRTHEVSEGTRILEAASSVGIQIPTLCHDPRLNPSGV
                     CRLCLVEIEGSSKPVPACRTEITSDMHIETHSAHIEKYRTDLLKMLHARYPQEVVEAF
                     PEKELHRLFQHYVPRFEEPVTVKSSWKDTTHPYIRIDMSLCVDCHRCERICSEVQGQF
                     VWNPIQRGFATTMTPEGRPTLLESGCVSCGACADTCPSG"
BASE COUNT         1011 a          749 c          840 g          998 t
ORIGIN      
        1 tatccatatc cagattgatg gaggtattgg ggccaccggt aatgcggtac gtgttaagcg
       61 agtccacata atcgaattcc ccgtaaacag ggtgattgcc atgcacacgg gtagaggagg
      121 ggaattggtg cgtcatggtt acttgggttg atccttcgat ggacgtacga tgtacgtcgg
      181 taaataagat attttcattg tcaatcagcc atgttgtggt cgtgttacgt tggcaagggg
      241 gatctatgct gttttgactg agaagcctca tttggttttt gctgtttatg gagctgaata
      301 ttttttcgtc cccgcatcgt atgtctttgt aagaaatagc ataatgagca agaaggtgat
      361 agtttttatc cggagcagaa aattcgctaa accgatctga accgggcata tctcgcagtt
      421 cgttctttaa cgatcgtaag gattcattaa gttttgaacg cgaataccag acaccgcgtg
      481 acatgacacc gttgtgatgg gccagggttg tgattttttc aagtgggttt tcgggtacca
      541 gcaggagatc ggcttcttta cctatggaaa cggttccgat tttatcatcc catcccaaaa
      601 actccgctgc cgatgaagtc ccggatttaa gtatgtcgta atggggaatg cccagctcgg
      661 caaaattttg aacctcttct aaatatgaaa aaccatgtat ggcaaaatga aatccggcat
      721 cggatccgat caaaagcttt acgccgtttt cataaaatac tttaaccact tcggatgcac
      781 cggagtagcg gtaattgagt gaattcagat gccaatatac tctctgccgc ggcggtacat
      841 aatccatttc cgcgcgtaat tttaaactat cgctcttatg cgccaaagaa cgtgctttgt
      901 gaattgtcag cgtcgggata acatattttc cgtttttacg aaatgcttcg gcaaattgaa
      961 taagttgttc tttaggatag gtatcaccgc ttcgcggatt gagtaaacct accaggttgt
     1021 cgaacgataa ttgagaacct tcagcatact cttcgaatgg gatcatatag ggaacgtgac
     1081 cttgcacggg aagaccttgt tggcgtgctt cgtccaaaac ggccgaataa atatcgggcg
     1141 taatcgtgac atacgaaaat acagattgat aacccatgcg tttcacgtca cgaacggcga
     1201 atcgcgcttc atcggcattg aggagcatgc cttgagtatg gtaggctaca acggatcctg
     1261 taacggccgg gcccgtgctg taaatacgcg ggccttgtag tttaccggta tcagagagtt
     1321 ttttgatcgc atgctgaaat gctgcgcccg gcacattacg gaccgttgta attccgtttg
     1381 caagaaaaag catcaagttt ttttcatgaa tatgcgcata gcagtcaatc aaccccggta
     1441 tgagatattg ccctttgccg tcgatgatcg aagcttcttt gggtatggat acttcacgcg
     1501 tattgccgat agctgcgatt ttaccgtcgg tgataatgac agtttgatga tggagtattc
     1561 gaaagctatc catcggtatg acatggacgt ttgtgaacgc gtgtgtttga gatagagcta
     1621 caatcgagaa tgtagaagac aaatttaaac ctataccgat taaaagaagt agtttcatac
     1681 gaacctatta aaataaaatt gaaaaaaaat tatttagaaa acgtatacgg caaagctctg
     1741 tatcgttcgt tctgctggag agcattcgga taggcggttg aatttttaaa aagagtgcgt
     1801 atggattgtg cattgatatc gggaagcccg gttggcgcgg taactacatc aaaatggaga
     1861 tgaggaatac ctagcgtata accgctatta ccgctcaaag ccacggtgtc acctttggca
     1921 actgtagcgc ctgttttgac caatgcgcca tgcttgcgga tatggatata acgggaatac
     1981 gtcgcatcgc gatgacgtat gacaagtaca ttttcgtgac ccaacgtgtg atcgctatct
     2041 tcaaaattat cgcttataaa tactacttcg ccttctcgtg acgctgtcaa tacagttccg
     2101 accggcatat caaaatcata ggcatatcgg aaaactccgt taaaattatt cggtgaatgg
     2161 ctccagatcg tgttgttact ttgagaaacc ttgtatcgtt tacctacagg aaatggcagc
     2221 acgaaatccg atgtatcggc aacaaccact gtcgaatcgg ttgcctcgcc atcttgacaa
     2281 ctgccaaaac taaagcttcc caacgaaata caagtgatca taatcaaaac atgcataaca
     2341 actccggtta cgaaagactt atccattgcg gtgatttccg tggtaaacga atgattaatt
     2401 tgcgtgcttc aagatcaagt ttgacagtga cgatatacca cactacgcta ccctcaaaat
     2461 ccgacgatag tgtccttaca gcatggtcag tcaattcttc aaaaggcata ggtcctcttt
     2521 cctttaatgt taaaacaata aaaccgcgta tcacatcata tttggatttg gctattcgta
     2581 cgccttgttt gccttgcgga tgccgtgtta atatggtgga tacttttttc atgtatgatc
     2641 tccgcgtttt taggtgtctc tagaggggca aatgataacg aataaaaacc catatccgtc
     2701 tgattgtcgt ttgtaaggac acaagaaatt catgtaacca aagaatagat cttgattgaa
     2761 aaatacgacg atacggcagc aagcaaaagc aaatggcgtc agaatgcatc agatataggg
     2821 tagagtgcat gaaatggcct ttgagccgcc aaggttactc aagcaaaaat taatatagag
     2881 ctatattgtt cattggttat cgtatgataa aaatcatcat gtttttatga aaaaaagttg
     2941 catattcccc tagttcaagc acatcaagaa cataacttcg ttaccaaaag ggtgaagatt
     3001 atgatcaaat ttaccatcaa tggccgtacg catgaggttt cagaaggaac ccgtatttta
     3061 gaggccgcgt cctctgttgg aattcagata ccaacactat gtcatgatcc gcggctcaac
     3121 ccgagcggcg tttgccgttt atgccttgta gaaattgaag gatcttccaa accggtaccg
     3181 gcatgccgta cggagatcac gtccgatatg catatcgaaa cgcatagcgc tcatatcgaa
     3241 aaatacagaa cggatctatt gaaaatgctt catgcgcgtt acccgcaaga agtggtggag
     3301 gctttccctg agaaggaatt gcatcgtttg tttcaacact acgtgccgcg ttttgaagaa
     3361 ccggttacgg tcaaatcgtc atggaaagat accacacatc cttatatccg cattgatatg
     3421 agtctgtgcg tagattgcca tcgctgtgag cgtatttgca gcgaagtgca gggacaattc
     3481 gtatggaatc ccatccagcg tggttttgcg accacgatga cgccggaagg tcgtccgacg
     3541 ttattggaaa gcggttgcgt aagctgtggc gcgtgtgcgg atacatgccc gagcggtg
//
LOCUS       DAOQGF010000209         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14932_length_3562_cov_7.140861, whole genome shotgun sequence.
ACCESSION   DAOQGF010000209 DAOQGF010000000
VERSION     DAOQGF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14932_length_3562_cov_7.140861"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PK937_15820"
     CDS             <1..723
                     /locus_tag="PK937_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNH34090.1"
                     /translation="RCDVRIERTVTQVKAMNIIGKIEGHDPVLKNEYVAIGAHFDHLG
                     VNASGAIFNGADDDGSGTAAVLETVRSVFASQQNKRSVLVVFHGAEEKGLIGSKYLTD
                     PATVNAWTWKDVTAQINMDMVGRESADSIYVIGSGRLSSQLQSTNETINRGTGYFYFD
                     YTFDRDDDPNQYYYRSDHYNYAKLGIPIVFFFDGMTSDYHKPSDDVAKINFKKLYKIT
                     DHTRRLLMNLSNRPERLVVDKH"
     gene            727..1320
                     /locus_tag="PK937_15825"
     CDS             727..1320
                     /locus_tag="PK937_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34091.1"
                     /translation="MFLRRKEINIYEFLRPGSRGHGYVVNFHRKYFDVFRGTDLENQD
                     AFINEVFIGLSSIREESIREKADHYLMKAVYFRCWRLFEDNKAYKQSEVPASRAQRDD
                     EDDDAVTRAPSTTPLPDSPLLAQDLLEHIHRFRMTLNPREIVVLNGLIDEVSIAEIAR
                     KAGVTANYAGVLIHRVRESLHVYLEDIGYFKKNKKKL"
     gene            1500..2435
                     /locus_tag="PK937_15830"
     CDS             1500..2435
                     /locus_tag="PK937_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34092.1"
                     /translation="MSKHERDWFDTQIREEDSARNLFEQVFDDDILLDPSYRTESPLI
                     QKMSKGIQIRCATGLTLQIDQSMALISEAGPAWIGQSALIKSVGWSLRILEVQTGLRL
                     PCALLSEQFSSELKVCSVPLADKEDSVHTDRPVWYIWSAAATIVVMIMAGMWWLQKPT
                     EVLTTDTKLDISKDTLVVPQRNFTEQPQVELPLRQVPKPNKEKLLAAHFEPNAVLENF
                     IGRTYRAEVRIEIIQPSSGDTLRGPLVFTWKKSDPNVNVTVNILNNRNASIWEANTKE
                     DQLSYNGVLAPGLYYWIIKSGDVRLDVRKFYIPIQ"
     gene            complement(2429..>3562)
                     /locus_tag="PK937_15835"
     CDS             complement(2429..>3562)
                     /locus_tag="PK937_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNH34093.1"
                     /translation="IAEIQHTLKKDAAVLIDYFTGDSITTVITVTATRISMQVLHNDT
                     TLESSVRIIRKHVNNLASDELSQSSYSFYKRWVQPSLQSVKLGATLVIIPDGSLSYLP
                     FETLVTSSVSSNLTSKVRYLCQDFPIQYVFSADRIITTSDKTPTNAFFGFAPVFSDTT
                     ISGYIKEYQFDDSTMSSHRGFFTRGTHFAQLPHSQTEVTNIQELFSNRKFNSKIVTHQ
                     KAVKSVLTSDSLTNYGFIHLASHGFINEANPKLSGIALYSDDSVDVKEDVLYAGEIYN
                     LRLNAHLVTLSACKTALGKIEKGEGVIGLTRGFIYAGARHVLVSLWPVDDRLTAEFMT
                     EFYRNVFTEKNLSLALQKTQKRWIAQQHTSYPGDWGAFVLIGY"
BASE COUNT         1034 a          687 c          821 g         1020 t
ORIGIN      
        1 cgttgcgatg tgcgtatcga acgcacggtt acacaagtga aagcgatgaa tattatcggt
       61 aaaatcgaag gtcatgatcc ggtattgaaa aatgaatatg tggctatcgg agcgcatttt
      121 gatcatttgg gagtcaatgc gtcgggcgcc atatttaacg gagcggatga tgacggttcc
      181 ggtacagccg ctgttttgga aacggttcgt tcggtatttg cttcacaaca aaataaacgt
      241 tcggtattgg ttgtatttca cggggcggaa gaaaaagggc tgattggttc aaagtatctg
      301 accgatccgg ccaccgtcaa cgcgtggaca tggaaagacg tcacggcgca aattaatatg
      361 gatatggtag gaagggaaag cgccgatagt atttatgtga tcgggtcggg ccgccttagt
      421 tcacaactcc aatcaaccaa tgaaactatt aatcgaggaa cgggctactt ttattttgat
      481 tacacattcg atcgtgacga tgatcccaat caatattatt accgtagcga tcattataat
      541 tatgccaaat taggtattcc cattgttttt ttctttgacg gcatgacgtc cgattatcac
      601 aaacccagcg atgatgttgc taagatcaat ttcaaaaaat tatacaaaat caccgatcat
      661 acgcgccggc tattgatgaa tctgtccaat agaccggagc ggcttgtcgt ggataaacac
      721 taagcgatgt ttctccgtcg taaggaaata aatatatacg aatttctgcg ccccggatcg
      781 cgcgggcacg gatatgttgt taattttcat cgtaaatact tcgatgtatt tcgcggtaca
      841 gacttggaaa atcaggatgc attcatcaat gaggtattta ttggtctgtc ttctattcgc
      901 gaagagagca ttcgcgaaaa agccgatcat tatctgatga aagcggttta ttttcgttgc
      961 tggcgtttgt tcgaagacaa caaagcatac aaacagtccg aagtaccggc gagccgtgca
     1021 caacgcgatg atgaagatga tgatgccgta acgcgtgcac cctctacgac cccactacct
     1081 gattcacctc ttttagctca agatttgctt gaacatatcc atcgttttcg tatgacacta
     1141 aatccgcgag agatcgtggt tctcaatgga ttgatcgacg aggtttccat cgctgaaatc
     1201 gctcgaaaag cgggggtcac tgcaaactat gccggcgtac tgatacacag ggtacgtgag
     1261 tcgcttcatg tgtatttaga agacatcggg tatttcaaaa aaaataaaaa aaagttgtaa
     1321 gtgttttgtt ggtattcgta cttactgttt gtgaggagtt agcttagtgc aaaaaaggcc
     1381 gtattatgac agatgatgaa aaaaaaataa atctagattt tttggacccg ttacgtcgga
     1441 gtccgtccgt gatatggaat gccgagatga tgagtcgttg tattcacggg tactccgtta
     1501 tgagtaaaca tgaacgggat tggtttgata cgcagattcg tgaagaagat tccgcccgta
     1561 atctttttga acaagtattt gatgatgata ttcttttgga tccctcctat cgcacagaat
     1621 ctccgctcat acaaaaaatg agcaaaggaa tccaaatacg ctgtgcgacg ggactcacat
     1681 tgcaaataga tcaatccatg gcgttgattt ctgaagcggg tccggcgtgg atcggtcaaa
     1741 gtgcattaat taagtcggta gggtggtcct tacgaattct tgaagtacaa acagggttac
     1801 ggttgccatg cgcattgcta tctgaacaat tttctagtga acttaaagtt tgtagtgttc
     1861 cgcttgccga taaagaagat tcagttcaca cagataggcc tgtatggtat atttggtcag
     1921 ccgctgcaac gattgtcgtg atgatcatgg ccggtatgtg gtggctgcag aagccgactg
     1981 aagttttaac gacggatacg aagcttgata tttccaaaga tacactcgtg gtgcctcaac
     2041 gtaatttcac ggaacaaccc caagtcgagt tacctctgcg tcaagtacca aagccgaaca
     2101 aagaaaaatt attggctgca catttcgaac cgaacgccgt tcttgaaaat tttatcgggc
     2161 gtacataccg cgcggaggta cgtatagaaa taatccagcc ctcgtctggg gatacgcttc
     2221 gcgggccttt ggtttttact tggaaaaaat cggatcctaa tgtaaatgtt acggtaaata
     2281 ttttaaacaa ccgtaacgca tcaatatggg aagctaatac gaaagaagat caattatcat
     2341 ataacggtgt attggcaccg ggattgtatt attggattat caagtccggt gatgtgcgtc
     2401 ttgacgtgcg aaaattttat atccctattc agtaaccgat caaaacaaat gcaccccaat
     2461 ctccggggta ggatgtgtgt tgttgtgcta tccatcgttt ttgtgttttt tgaagcgcca
     2521 gactcaggtt tttttcagta aacacattac gataaaactc tgtcataaat tccgccgtta
     2581 agcggtcatc taccggccag agtgaaacaa gcacgtgtct tgctccggcg taaataaatc
     2641 cccgcgttaa tccgatcacc ccttcgccct tctcgatttt tcccagcgcc gttttacagg
     2701 cactgagtgt taccaagtgt gcattgaggc gaaggttgta gatttctcct gcgtataaaa
     2761 catcttcttt cacgtcaacc gagtcatcgg agtaaagagc tatgcctgat aatttcggat
     2821 ttgcttcatt tataaaaccg tgactggcta agtgtataaa cccataattt gtaagactgt
     2881 ctgatgtaag aacggattta accgcttttt gatgagttac gattttggag ttgaatttcc
     2941 gattcgaaaa taattcttga atattagtaa cttcagtttg actatgagga agttgagcga
     3001 agtgagtgcc tctggtaaaa aaaccgcgat gtgatgacat cgttgaatca tcaaattgat
     3061 attccttaat gtaaccgctt attgtcgtat cggaaaaaac aggtgcgaat ccaaaaaaag
     3121 cattggttgg tgttttgtcg gaagttgtaa tgatacgatc cgcagaaaaa acatattgaa
     3181 ttgggaaatc ttggcatagg tatcgcactt tcgaagttaa attcgaagaa acagaagaag
     3241 ttactaaagt ttcaaatggc aggtaagata aactaccatc cggaatgata actaaagttg
     3301 cccctagttt aacggattga agtgaaggtt gcacccatcg tttgtaaaaa gaatatgaag
     3361 actgcgaaag ttcatctgac gcaagattat ttacatgttt acgaataata cgaacggatg
     3421 attctaacgt tgtatcatta tggagaactt gcatagagat acgcgtggct gtaaccgtga
     3481 tcactgttgt tatagaatcg ccggtaaaat aatcaattaa tacggcggca tctttcttga
     3541 gggtatgttg gatttcggct at
//
LOCUS       DAOQGF010000210         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_14985_length_3554_cov_6.656473, whole genome shotgun sequence.
ACCESSION   DAOQGF010000210 DAOQGF010000000
VERSION     DAOQGF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14985_length_3554_cov_6.656473"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..2557)
                     /locus_tag="PK937_15840"
     CDS             complement(182..2557)
                     /locus_tag="PK937_15840"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNH34094.1"
                     /translation="MYRLIFLLLLSSLSYAQKNITGAITDKSNGEPLPSANIQIEGTL
                     RGVVASVEGRYALRIESLPATIVVRYIGYESQKLVITENSPSEQNISLTPSSVQMEEM
                     IVIGEDPGIRIMRNVIAKKKRWHKLINNFKADGYNRMVMENDSGIVMISESLADTYWD
                     RDKGLKVVIKSKRMTKNVRPQDNIFVGEEGMVNFYDDDIEIQGSRFVGPTHPDALDYY
                     DFKITGRRYLDDYLVYDIAVRSKTKLQPTFIGSISVIEEDSAMVAVDLKPTEHVIFPM
                     PIQQWNVNYKQQFSNFGQAYWLPVDIRMHGNIKVAMPGLEFPKIIYSSLMGVNNYQIN
                     IELPDSLYKSRKSFFVDSVALAKPNQFDSSAIIIPRTEAEDSAFTNIKRGDSFTKAFK
                     PKGALARYVKMEDDYNDSVDTEKKKKTAFQKFLSDLSPVVGFDRVEGGRLGVKYEHEF
                     SKHWSAYIKGGYTTTLEKAFYGAGGEHRFGYRRRGHLEAEYYNGVDMRQNSENYNLLM
                     TSGQPLFGYRDYFDYYKSHRFKVTGGYNFAFQDLDFSAGFHQDIQKSVSKTTDWDIIG
                     RKYDQRKNPAIDKGTLRSFIFTGTWGEKYVPMGVVGNNGLTVSIEHSDKKILSSDFGF
                     TTYKVSGNVRIKTFLRRRFIPNTLDVYATAGTYTGKLPVQRFGVIDGSLGYFTPFGTF
                     KTLRTRGYEGEKYAAVFAEHNFRSLPFELIGLDWFARKNIGIAVFGSAGRTWIDKNRL
                     AGLGYAPVYTTRWHQEVGASINGLFGLFRIDAAQRLDKKNFYLGMSMARLF"
     gene            complement(2696..>3554)
                     /locus_tag="PK937_15845"
     CDS             complement(2696..>3554)
                     /locus_tag="PK937_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34095.1"
                     /translation="GLGAAGRVQRLIAQRGVDAAINEIEFQENNYARQIFCESIMAHE
                     NLTSAQLQRTVIVTAKEISSSSRLGDLLTDATTKFPQDSTFTLTLMRAVREISSSSRQ
                     SETAITIARRRGLDRESAIEMARTIEDVSSSSAQKNALETLAELAPPHEEAFLAYLHA
                     VRTVSSSSAQSEALLALTQRTLTRDAWIQLFKVTESVSSSSAQARVLSRSAEVCPNDD
                     MIWFTFLETVGTVSSSSAQETALSAMLRRQGLSKSVLKRTLQSVENSVSSSSVQRYIE
                     GLVHDRLME"
BASE COUNT          941 a          796 c          816 g         1001 t
ORIGIN      
        1 gcttggtaaa gctatgcgca aagcctttgc ttcctatcat caggatgtaa agagtaaccg
       61 atttcccacc gacaaagaaa gttattcgta gtagtttttt taaacccgtt attttttttc
      121 aaaaaaaaag agtgaaaacg attaattttc actctgctaa tttttgttat gtattgtttg
      181 tttaaaacaa gcgcgccatg ctcattccaa gataaaaatt ctttttatcc aaacgttgtg
      241 cggcatctat gcggaataaa ccgaataagc cgttgattga agcgccgact tcctgatgcc
      301 aacgcgtcgt ataaaccggt gcatagccca atcctgctaa acgattttta tcaatccatg
      361 tgcgcccggc tgatccgaaa acggcgatcc ctatattttt acgagcaaac caatccaatc
      421 ctatcaattc aaacggtagc gagcgaaaat tatgttccgc aaaaacagca gcgtattttt
      481 caccttcata tccgcgtgta cgtaatgttt tgaacgtacc aaatggggta aaatatccga
      541 gcgacccgtc aatcacacca aaacgttgaa cgggaagttt gccggtgtag gtacctgcgg
      601 tagcatatac atccagcgta ttggggatga aacggcgacg gagaaatgtt ttaatgcgca
      661 cgtttccgga aactttatat gtagtaaaac cgaaatcgct ggagagtatt tttttatccg
      721 aatgttcgat ggagaccgta agcccgttgt tcccgactac gcccatgggc acatattttt
      781 ctccccaggt acctgtaaaa ataaaactgc gcagtgtacc tttatcaatg gcagggtttt
      841 tccgttgatc gtatttccgt cctatgatgt cccagtccgt ggttttggaa acggattttt
      901 ggatatcctg atgaaatcct gcagaaaaat ccaaatcctg aaaagcaaaa ttgtaaccac
      961 cggtgacttt gaagcggtgt gacttgtagt aatcaaaata atcgcgatat ccgaagagcg
     1021 gttgcccgct ggtcatcagg agattatagt tttccgagtt ttggcgcata tctacaccgt
     1081 tataatattc ggcttcgagg tgaccgcggc ggcgataacc aaagcggtgt tcaccaccgg
     1141 cgccatagaa cgctttttcc aatgttgtcg tatagccgcc tttgatgtat gccgaccaat
     1201 gtttggaaaa ttcatgttcg tatttcacgc caagccgtcc gccttcgaca cgatcaaaac
     1261 cgacaacggg tgaaagatcg ctaaggaatt tttggaacgc tgtttttttc tttttctccg
     1321 tatccacgct gtcattgtaa tcatcttcca ttttgacgta tcgcgccaac gcccctttgg
     1381 gtttgaatgc tttggtaaag gagtccccgc gtttgatatt ggtgaatgct gaatcttccg
     1441 cttcggtacg gggaataata atagccgagg aatcaaattg attaggtttt gccaacgcga
     1501 cggaatctac aaaaaaagat ttacgcgatt tgtagagtga atccggtaat tcgatattga
     1561 tctgataatt attaacgccc atcagactgc tgtatatgat ttttggaaat tccaaaccgg
     1621 gcattgcgac tttaatattg ccgtgcatac gaatatccac cggtaaccag taggcttgac
     1681 cgaaattgga gaactgctgt ttatagttca cattccattg ctgaatgggc atcggaaaga
     1741 tcacatgttc cgtcggtttg agatctacgg ctaccatcgc agagtcctct tcgataacgg
     1801 aaatactgcc gatgaaggta ggttgtaatt tagttttgga acgtacggca atatcgtaaa
     1861 caagataatc gtccagatag cgacgtccgg tgattttgaa atcatagtag tccaatgcat
     1921 cggggtgcgt aggtccgaca aaacgcgatc cctggatttc gatatcgtcg tcataaaaat
     1981 tgaccatgcc ttcttcgccc acgaaaatat tatcctgcgg tcggacgttt ttggtcatac
     2041 gtttggattt gataacaact ttgagtcctt tgtctctgtc ccagtaggta tcggctaaag
     2101 attcggaaat catcacgata cctgaatcgt tttccatgac catcctatta tagccgtcgg
     2161 ctttaaaatt attgatcagt ttatgccagc gttttttctt ggcgatgaca ttgcgcatga
     2221 tacgaatgcc gggatcttcc ccgatgacga tcatttcctc catctgaacc gaggagggtg
     2281 tcaggctgat gttttgttcg gaagggctgt tttccgtaat gactaatttt tgagactcat
     2341 aaccgatata acgtacgacg atcgtagcgg gtagggattc gatccgcaat gcgtatcgtc
     2401 cttcgacact ggcgacaacc ccgcgcagcg tgccttcgat ttgtatattg gccgatggta
     2461 aaggttcacc gttacttttg tcagtgatcg ccccggtgat gtttttttga gcgtaggaaa
     2521 gtgaactcag cagcagaaga aaaatcagtc tatacatgtg ataaacccct cataatatgt
     2581 aatacaaacg ccgaccccat gtacgtgtaa gtcggcgtga agttccaata caaagtttta
     2641 taaattaaaa gaactcatag atagattctt ctcttatact ttgaggaatc gacgcttatt
     2701 ccattaagcg atcgtgtacc aaaccttcga tataacgttg tacggaagaa ctcgacaccg
     2761 aattttcaac cgattgtaaa gtgcgtttga gaacggattt gctcaagccc tgacgacgca
     2821 gcatggcgga aagagcggtt tcctgcgcgg aacttgagct tacggttccg acggtttcaa
     2881 gaaaagtaaa ccaaatcata tcatcattgg ggcagacttc tgcgctacgg cttagcacac
     2941 gcgcttgtgc gctggacgaa ctgaccgatt cggttacttt aaaaagctga atccacgcgt
     3001 cgcgagtaag tgtgcgttgt gtaagcgcga gtaacgcttc ggattgtgcg ctggatgaac
     3061 ttaccgtgcg tacggcatgc aggtatgcca aaaacgcttc ttcatgcgga ggcgctaatt
     3121 cggcgagcgt ttccaatgcg tttttttgcg ccgaactgga gctgacatct tcgatcgtgc
     3181 gggccatctc gatagccgat tcacgatcta agccgcgacg gcgcgctatg gtgatagccg
     3241 tttcactttg acgagaactg gaggatattt cgcgtacggc acgcatcagc gttaacgtaa
     3301 aggttgaatc ttgcggaaat ttagtcgtgg cgtccgttaa taaatcaccg aggcgcgaac
     3361 tgcttgagat ttctttggcc gtcacaataa cggtgcgctg aagctgagca gaggtcaaat
     3421 tttcatgtgc cataatggac tcacaaaaaa tctgccgcgc gtaattattt tcctgaaatt
     3481 cgatttcatt gattgccgca tcaacgccgc gctgagcgat aagccgctgg acccgacccg
     3541 cagcgccgag tcct
//
LOCUS       DAOQGF010000211         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_15218_length_3518_cov_6.231014, whole genome shotgun sequence.
ACCESSION   DAOQGF010000211 DAOQGF010000000
VERSION     DAOQGF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15218_length_3518_cov_6.231014"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1623
                     /locus_tag="PK937_15850"
     CDS             274..1623
                     /locus_tag="PK937_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34096.1"
                     /translation="MRSNLMRFAGIVALPLVFLAFLFINSCEGPRGPAGADGKDGTNG
                     TNGTDANETCKTCHNPNDVDRIKIEFQFSKHEYGEAAFEEAGNTSCAVCHTQQGFLYV
                     VQNNVPSTFTLNAGTGKYSNNYATISSQALGEIGCGTCHASTHTTYGAADTVLTTNAA
                     VAMTMWKGNKTINLAQDNGRSNLCIKCHQPRPMTKSTTLSNGDIVNYDSLALDPNRMF
                     YDSTTGASNTHTYPSYRMHVHYGVVGAVFAGMGGVEFTGSKTVQSSTHTSVASCQDCH
                     MATVSGRGGGHTFMVQGNFNGCNTSGCHTGVSSSNSTLWTNPRNTIKGLLNTLATKIN
                     AAGGGTPILHADSDTETNLWAGLTTGNWDGYLNIYDPSSNPGGAWRNPNPSGSWTQAQ
                     KDANLALPKFPSLKYVQVGAMINFQFALREASLGIHNYKYSEALLTNTIEKMTDAGF"
     gene            1967..3340
                     /locus_tag="PK937_15855"
     CDS             1967..3340
                     /locus_tag="PK937_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34097.1"
                     /translation="MNIKKRLVGYLIVLCGAVVLFDASAQTLSTRLSTSVYSWENQYA
                     DSTTQKSFRGYQTAIFHANGIWKSNISLHGYVTGSYDLAGARQDNPDYRVYNLYAQWK
                     SDPTKNHRWEARVGRQQVYAGVRAPSIDGVRYDFYNADKYGFMAYYGALPPADGTVKM
                     GRPADRSAFGAKVMTSKLAQTHMALTYVERRSIGQYYYTINGRNADTLVYSPDLDERL
                     IAFDANRNFFGKINWFYNVTYDALASNVRKISTDVQFQPLHTLWFAVQYAYRRPDLYY
                     NTIFAKFDDIEANQELWVRGTYQFNTDWSFNGEYANVFYKMKDAWRYGAGLTYQRTNV
                     SYQWRLGYGGTIKQISLSSYYPVSRSMYLYGGLTYATYDMKDDITSSDFLSSSTQDIY
                     RQVYQYTNSSMTAVLRCNYNLMQAMNVDVEGQYLSQNIKTSPVFAGSKYDLRLFVRAN
                     YWFFNKI"
BASE COUNT          986 a          747 c          800 g          985 t
ORIGIN      
        1 agaaaaagta tgatcaaaac taagtagctt gataacaagt gatcatttta atcattgaaa
       61 gcgggccaaa gcccgctttt tttataatta ttttttatga tttgtaataa tttagtcaaa
      121 aaatgagttt taaaatattg ttaaaacttt gtttatgagc gaaaattttt tatgacaaat
      181 ttatgacaat tatcataggg atggtatgga cgtgtgttta ttttatatgc gagattcaac
      241 tccaaaccaa tcctaagtag gaggtaaaaa gcgatgagaa gtaacttgat gagatttgca
      301 ggcatcgtcg cactaccatt agtattttta gcgtttttgt ttatcaatag ctgcgaaggc
      361 ccgagaggcc ccgccggagc ggatggcaaa gatggtacaa atggcacaaa tggtactgat
      421 gcaaacgaaa catgcaaaac atgccacaat cccaatgatg tggatcgtat caaaattgaa
      481 ttccagtttt ccaaacatga atacggcgaa gccgcttttg aagaagccgg caatacatct
      541 tgcgccgtat gccacacgca acaaggattc ctgtatgttg ttcagaacaa tgttccgtca
      601 acatttacgt tgaatgcagg taccggcaag tattccaata actatgctac catttcttcc
      661 caggctttgg gtgaaatcgg ttgcggtaca tgccatgcca gcactcatac gacgtatggt
      721 gctgcggata ccgtattaac gacaaatgcg gccgtcgcga tgacgatgtg gaaaggcaat
      781 aagacgatca atttggctca agacaatggt cgttctaatc tttgcatcaa gtgccatcaa
      841 ccgcgtccta tgacaaagag cacgacgctc agtaatggcg atatcgtcaa ttatgattct
      901 ttagcattag atccgaatcg gatgttctat gattcaacga cgggtgcttc caatacgcat
      961 acctatccca gctatcgcat gcatgtgcac tacggtgtag taggtgctgt atttgcaggt
     1021 atgggtggtg tagaattcac aggatctaaa acagttcaaa gttcaacgca cacttccgtt
     1081 gcgtcctgcc aagattgtca catggcgacg gtcagtggtc gtggtggcgg acacacattt
     1141 atggtgcagg gtaactttaa tggttgcaat acctccggtt gccataccgg cgtaagttcc
     1201 agcaactcta ccttgtggac aaatcctcgg aacacgatca aaggtttgct caatacattg
     1261 gctacgaaga tcaatgcggc cggcggcgga acaccgatcc tccacgctga cagtgatact
     1321 gaaaccaatt tgtgggcagg tttgacgaca ggcaattggg atggatatct gaatatatat
     1381 gatccttcat ccaatcccgg cggggcatgg agaaatccca atccaagcgg ctcatggaca
     1441 caagctcaaa aagatgcaaa cttagcatta ccgaagttcc catcgctgaa gtatgtacaa
     1501 gtcggtgcga tgattaactt ccagtttgca ctgagagaag ccagcctcgg tattcacaac
     1561 tacaaatatt cggaagctct gctgacgaat acgattgaaa aaatgacgga tgcaggcttt
     1621 taattagtac gatcgttacg attcttcaaa ggtcgctcgc taaaacgagc gaccttattt
     1681 tttatctatt ttccgctttg ttccacaaat tttaagcaaa aaccttctga tgcttaaaat
     1741 caaacgaccc ataagttgtt aaaacgctgg ttacatcagt ttgttgactg atgaaattaa
     1801 tgcaaagtaa tgctgtaatt ttctttacaa ttttatgaca attgtcatag ggtgtattgc
     1861 gacttgggca tatcttttga tgaaataagt gcctttctgt atttttatta gctttatttt
     1921 gacatataat ttacgacgat tcatggactt gaagcctgag gttgctatga atatcaaaaa
     1981 aagattagtt ggctacttga tagtgttgtg cggcgcagtc gtactttttg acgcatcagc
     2041 ccaaacgctt tcaacgcggc tttcgacatc ggtgtattcc tgggaaaatc agtatgcgga
     2101 ttcaaccaca caaaaaagtt ttcgtggtta tcaaacggcg atttttcatg ccaacgggat
     2161 ttggaaatcc aatatttcgt tgcacggata tgtaaccgga agttatgatc ttgccggtgc
     2221 gcggcaggac aatccggatt atcgcgtgta caatctttat gcgcagtgga aatccgatcc
     2281 gacaaaaaat catcgttggg aagcccgcgt aggacgccag caggtttatg ccggtgtacg
     2341 tgcgccttcg atagatggtg tccgttacga cttttataac gccgacaaat acggatttat
     2401 ggcttactac ggtgcactgc ctccggccga tggaactgta aaaatggggc gcccggcgga
     2461 tcgttccgcg tttggtgcca aggtgatgac ttccaagttg gcgcaaactc atatggccct
     2521 aacgtatgtt gaacgacgca gcattggtca atattactat acgatcaatg ggcgcaacgc
     2581 ggataccctc gtttacagtc cggatttgga tgagcggctc atcgcttttg atgccaatcg
     2641 caattttttc ggaaagatca attggtttta taatgtgacg tacgatgcgt tggcctctaa
     2701 cgtgcgtaag atatctacgg acgtgcaatt tcaaccgttg catacgctgt ggtttgccgt
     2761 acagtatgct taccgccgac cggatcttta ttacaatacc atttttgcga agtttgatga
     2821 cattgaggcc aatcaggaac tgtgggtgcg cggaacgtat caattcaata ccgattggtc
     2881 gtttaacggc gaatacgcca acgtatttta caaaatgaaa gatgcttggc gttacggcgc
     2941 gggcctcacg taccaacgca caaacgtatc ttatcaatgg cgtttggggt atggcggcac
     3001 gatcaaacaa atttcgttaa gctcgtatta tcctgtcagc cggagcatgt atttgtacgg
     3061 cggcctcact tatgcaacat atgacatgaa agatgacatc acatcgagcg attttttgtc
     3121 aagcagcaca caggatatct accgtcaggt atatcaatat accaatagtt ccatgacggc
     3181 cgttctccgt tgcaattata atctgatgca ggcgatgaac gtggatgtcg aaggacaata
     3241 cctttcgcaa aacatcaaaa catcgccggt ttttgccgga tcgaaatatg acctgcgcct
     3301 ttttgtgcgc gccaattatt ggttcttcaa taaaatataa cttataacgg acactggtat
     3361 gaagaaacta ctcggaataa tactcattac ggcggtcgga ctcatggccg ctgccttggt
     3421 tttctttcct tcttcggagc cgctcaaagc ttcgtcgctg gtgaaaactt ccgctcagat
     3481 gcaagataag atcggcagcc gttatgcgat tatataca
//
LOCUS       DAOQGF010000212         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_16029_length_3410_cov_7.608942, whole genome shotgun sequence.
ACCESSION   DAOQGF010000212 DAOQGF010000000
VERSION     DAOQGF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16029_length_3410_cov_7.608942"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PK937_15860"
     CDS             <1..176
                     /locus_tag="PK937_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNH34098.1"
                     /translation="GQYSWWDQQSRARERNVGWRIDYFFVSDNLLPALKNAWIMPDVM
                     GSDHCPVGIELKF"
     gene            192..1064
                     /locus_tag="PK937_15865"
     CDS             192..1064
                     /locus_tag="PK937_15865"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNH34099.1"
                     /translation="MKNRFSIKDLEQFSGIKAHTLRIWEKRYDMLNPNRTKTNIRWYS
                     SDDLKRLLNIALLYHHDHKISKIAALTDKELADKVAEIAQNTSDYSVFVDGLVMAMIE
                     MDEQRFEKIFSSGLIKLGFEDTIAKVVYPFLIKIGFMWQMGSSNPAQEHFVSNLIRQK
                     IIAAIDGQVVPMDRSPKKAILFLPDGEMHELGLLVLNYMMRARGLNTYYLGQSVPMED
                     LRRACEIKKPDYLVSVFTSPMPKLKEFVRLLSQHFPHTKIILSGKQILSANLKLPSQF
                     YLLPSFDQLNKHLS"
     gene            1215..1949
                     /gene="folE"
                     /locus_tag="PK937_15870"
     CDS             1215..1949
                     /gene="folE"
                     /locus_tag="PK937_15870"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNH34100.1"
                     /translation="MRTQPDFRIPLQVLNNTTAGAREENGHIDLIEDDFGDDHISSAV
                     ETPMRDDAFALNDEAKIELIEYHFKKIMHVLGLDLTDDSLKGTPRRVAKMYVQEVFQG
                     LNPKNKPSVTLFKNQYHYNHMLVEKNITLFTYCEHHFVPIFGKVHVGYIPASQVIGLS
                     KINRIVQYFAKRPQVQERLTVQIAEELRRILNTEDVAVVIDAAHLCVSSRGVQDTEST
                     TTTSSFLGRFHDEGVRNEFLCYIELK"
     gene            1976..2548
                     /locus_tag="PK937_15875"
     CDS             1976..2548
                     /locus_tag="PK937_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNH34101.1"
                     /translation="MKQIIFLIVSINVLLFGIACRDVRIKPEQPNMKSTQSVYNIAVK
                     TIDGAETNLATYRGKKILLVNVASECGYTPQYKDLQKLQEQFGSKVVVLGFPSNDFGG
                     QEPGSNAEIKNFCERNYKITFPMFDKIAVVGEGQHPLYQWLSQKNLNGWNEKAPSWNF
                     CKYLIDENGELIGYYNSSVNPLSDEIINKL"
     gene            2561..3022
                     /locus_tag="PK937_15880"
     CDS             2561..3022
                     /locus_tag="PK937_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNH34102.1"
                     /translation="MHVLEREQTLSCTLLDAWQFLSSPQNLKLITPPYMGFEIRSDQN
                     GKPMHAGMIITYRVSPLFNIPMTWVTEITHVREPYYFVDEQRIGPYTLWHHQHFLEAV
                     EGGVRMRDCVHYKLPMGIIGNQIEKWIVRRRLESIFDFRWRKLNEIFNAPS"
BASE COUNT         1016 a          676 c          715 g         1003 t
ORIGIN      
        1 ccggtcaata ttcgtggtgg gatcagcaga gtcgggcacg cgagcgaaac gtggggtggc
       61 gtattgatta tttttttgtc agtgataatc tgcttcccgc actgaaaaac gcatggatca
      121 tgcccgatgt gatgggttcg gatcattgtc ctgtaggcat tgagttaaag ttttaatttg
      181 atcgggcacg tatgaaaaat cgcttttcaa tcaaagatct tgagcagttc agcggaatca
      241 aagcacacac tttgcgcatt tgggaaaaac gctacgatat gcttaatccc aaccgcacaa
      301 aaaccaacat ccggtggtac tcttcggacg atctcaaacg actgctaaat atcgctctgt
      361 tgtatcacca tgatcataag atttccaaaa tcgctgcgct gaccgataag gagctagcgg
      421 ataaagtcgc agaaatagcg caaaacacct cggattattc ggtttttgtg gatggtcttg
      481 tgatggctat gatcgaaatg gatgaacagc gttttgagaa aattttttca agcggactga
      541 tcaagcttgg ttttgaggat accatcgcta aagttgttta tccattcctc atcaagatcg
      601 gcttcatgtg gcaaatgggt tcgtccaatc ccgcacagga acacttcgtg tccaatttga
      661 ttcgtcaaaa aattatcgcc gcgattgacg ggcaagtcgt cccgatggac cgatctccca
      721 aaaaagcgat tctttttttg ccggatggcg aaatgcatga attgggtctt ttggtgctca
      781 attatatgat gcgtgctcga ggtttaaata cgtattacct tggccaatcg gtacccatgg
      841 aagatttacg acgagcttgc gaaataaaga aaccggacta cctggtatcg gttttcacca
      901 gtcctatgcc taagcttaaa gagtttgttc gcctattatc acaacacttt cctcatacca
      961 aaatcatact gagcggtaaa caaatattat ccgccaatct aaaattaccg tctcaatttt
     1021 atctgctgcc ttcgtttgat caattaaaca aacatctgtc ataagtattt ttttaatgag
     1081 ggcttgttta tttgttgaat ttttgtttaa tgtttaattt caaattttaa acaaaattat
     1141 tgacattatc cgaaccatat gatattttga cttagctttt tttaaagcta aacatcgaat
     1201 atcaaggaat taatatgcga acacaacccg atttccggat tcctttacag gttttaaaca
     1261 atacgacggc tggggcacgg gaagaaaatg gtcatataga ccttatcgaa gatgatttcg
     1321 gtgacgatca catcagttcg gccgttgaaa caccgatgcg tgatgatgcc tttgcgttga
     1381 atgatgaggc caaaatagaa ttgatcgaat accatttcaa aaaaatcatg catgttttag
     1441 gacttgatct tacggatgat agtttaaagg ggacaccgcg ccgggttgcc aaaatgtatg
     1501 tgcaggaggt ttttcagggt ctcaacccaa aaaacaaacc gtcggtaacg ctttttaaaa
     1561 accagtatca ttataatcac atgctggtag aaaaaaacat aactttattt acgtactgcg
     1621 aacatcattt tgttccgatt ttcggaaagg tccatgtggg ttatattccg gcgagccaag
     1681 tgatcggttt atccaaaata aatcgtattg tgcaatactt tgccaagcga cctcaagtgc
     1741 aagaacgttt gaccgtacag attgctgagg agttgcggcg cattctaaat acggaagacg
     1801 tggccgtagt tattgacgcg gcacatttat gtgtgtcatc gcggggtgta caagataccg
     1861 aaagtacgac gactacatcc agttttttag gccgtttcca tgatgaaggc gtgcgaaatg
     1921 aatttttatg ttatatcgaa cttaaataaa aaacgtttaa cacatctaga ctaatatgaa
     1981 acaaataatt tttttaattg tatccattaa cgtgctgttg tttggcatag catgccgtga
     2041 tgtacgtata aaaccggagc aaccaaatat gaaatctacg cagtccgtgt acaacatcgc
     2101 tgtcaaaaca attgacggcg ccgaaacaaa tttggctacg tatcgcggaa aaaaaatact
     2161 cctcgttaat gtggcatccg agtgtggtta tacgccgcaa tataaagatt tacaaaaatt
     2221 acaggaacag tttggatcaa aggtcgtcgt tcttggtttt ccgtccaatg attttggcgg
     2281 acaagaaccc ggctccaatg ctgaaataaa gaatttttgc gagcgcaatt ataaaatcac
     2341 atttcctatg tttgataaaa tagccgtggt cggggaagga caacatccgc tctaccaatg
     2401 gctcagtcaa aaaaacctca atgggtggaa tgaaaaggcg ccgagctgga atttttgtaa
     2461 atatttaatc gacgaaaacg gggaattgat cggatattat aattcttccg ttaatccgct
     2521 gagtgatgaa atcattaaca aattataaca aaatcacaca atgcacgttt tagagcgcga
     2581 acaaactttg tcgtgtacgt tgttggatgc ttggcaattt ttgtcttcac cacagaatct
     2641 caaattgatt acaccgccgt atatgggatt tgaaatccgc tcggatcaaa acggcaaacc
     2701 catgcacgcc ggaatgatta ttacctatcg ggtaagccct ttatttaaca tacccatgac
     2761 ctgggtaact gaaataaccc atgtgcgtga accttattat tttgtggacg aacagcgcat
     2821 aggaccttat acgctgtggc atcatcagca ttttcttgaa gcggtggagg gtggggttcg
     2881 tatgcgggat tgtgtacatt acaagttgcc aatgggaatt atagggaatc agatagaaaa
     2941 atggattgta cgccgtcgcc ttgaatccat atttgatttt cggtggcgta aattaaatga
     3001 aattttcaac gctccatcct aagccgtatc actgatttta tcgcactttt acttacgctt
     3061 atactcgcct cataattttt accagtcctt tatttgaatt cagatcgtac agatcgttaa
     3121 acgagcctga agtcacaacg tccaatcaat gaaaaggcat tttgccttgc ttgtttcctc
     3181 acagtctatt atattttaaa tcgttataaa ataatctttt attgatctac ggagcgtttc
     3241 aaggcgtggc cttattgaag tcatcttcag cgaattccga atttgatata ctcaaaatcg
     3301 tcgcaaagaa aaaactctca gaatcagatc atgcattggt acgtgctgaa ttgcaaaaac
     3361 ggtctatgcc tcgccatata gccattatta tggatggtaa cggccgctgg
//
LOCUS       DAOQGF010000213         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_16046_length_3408_cov_7.303310, whole genome shotgun sequence.
ACCESSION   DAOQGF010000213 DAOQGF010000000
VERSION     DAOQGF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16046_length_3408_cov_7.303310"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK937_15885"
     CDS             <1..190
                     /locus_tag="PK937_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34103.1"
                     /translation="SALTAEQIKRQYNGGYGNPPLDVPAYLIHEYDGTGGTSTSETDQ
                     SGSGLTGTLNNGVTRTTW"
     gene            184..390
                     /locus_tag="PK937_15890"
     CDS             184..390
                     /locus_tag="PK937_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34104.1"
                     /translation="MVKTIIGFFIGVLLTLGVQNYLTKGDEKNEITIDEKLIDSLDND
                     TKRDFLEERARSRKERKKDMGKND"
     gene            594..953
                     /locus_tag="PK937_15895"
     CDS             594..953
                     /locus_tag="PK937_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34105.1"
                     /translation="MQFLIAGLFGALLYIIFSALKAHPKDSLDAFIGYFKKHPLAIGA
                     SYLTYALTMFAWYANGLDSAVSWVAGVFALSYDGQFFPQGKFNFLAIGIGFASHVIAN
                     MLYMKLTNYHSKQAEAQ"
     gene            950..1201
                     /locus_tag="PK937_15900"
     CDS             950..1201
                     /locus_tag="PK937_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34106.1"
                     /translation="MTAPKFDNKISWGNIIYLVGLIFAVAVFYAKTTANDKTLEKITP
                     EVENLKTETEVLKTELKSINKKLDEQNDLLRELVFGKNR"
     gene            1536..2105
                     /locus_tag="PK937_15905"
     CDS             1536..2105
                     /locus_tag="PK937_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34107.1"
                     /translation="MTEQIRNINNTRLLSVDESIRDQVMMCLDRMHELEQPAVIHRDV
                     WRSPALQELKFRQGVSKLKWGYHCATRNGKPASLACDIVHIDHAWFSKFDATYKADEY
                     FWLCLGRSAKEAGLDWGGEWMWTWPSKEATEKVRKLNERIDTLDATGLETARDFGWDV
                     AHIEVRGVSAEEAAQGWRISNGVKFKIVP"
     gene            complement(2690..>3408)
                     /locus_tag="PK937_15910"
     CDS             complement(2690..>3408)
                     /locus_tag="PK937_15910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNH34108.1"
                     /translation="KSQPNESVNMIYCDILYGTGRNFGDYQDLKPIRSEIESHYLPRL
                     IEMKRVLKQNGVLVFQLDWRINHWMRILIDDVFGYENFRNEIIWKYRLGLKAKSKKFI
                     QNHDVILVYAKSSNHTFNEQKEKIKPRKKWFQKFENGKAVHQKDIPPILVDEQLIGSV
                     WTDIKMRGSKLLYPTEKPKELVQRIILAFTNEGDIVADYYLGSGTTAVVCKELNRNFI
                     GCDINPKAIEITKARLNGGQ"
BASE COUNT         1011 a          644 c          712 g         1041 t
ORIGIN      
        1 atcagcatta acggcggagc aaataaagcg gcaatataac ggcggatatg gtaatccgcc
       61 gttagatgtg ccagcttatt taatacatga gtacgatggc acgggcggta cgtcaacatc
      121 tgaaacagat caaagcggca gtggtttaac tggaacttta aacaatgggg ttacgagaac
      181 aacatggtaa aaacaataat tggatttttt atcggcgttt tattaacttt aggggtacaa
      241 aattatttaa ctaagggaga cgaaaaaaat gaaatcacta ttgatgaaaa gcttattgat
      301 tctcttgaca atgatactaa gcgcgacttt ctcgaagaac gtgcaaggtc aagaaaagaa
      361 agaaaaaaag atatgggtaa aaacgattga cagcgtctta gtcagcgaag acgttttaaa
      421 ccaagccgtg aaagatgcta tcgttgcgga taaatgcggc aaggaaatcg aaaggcaaaa
      481 aaaacaagct cgcactaacc gaatacgcgg ttggatagag ggttcggcgg tgtttctaac
      541 ttacatgatg tttaaatatt ggaaaaaatg aggtgcaaca gatgagcgca aacatgcaat
      601 ttttaatcgc ggggcttttc ggggcattgc tttacatcat attttcagca ttaaaggcgc
      661 acccgaaaga ttcgcttgat gcgtttatcg gctatttcaa aaaacatcca cttgccatag
      721 gcgcgtcgta tttgacgtat gcgctgacaa tgttcgcatg gtacgctaac ggcttagatt
      781 cagccgtgtc atgggtggcc ggagtgtttg cgcttagtta tgatggacaa ttttttccgc
      841 aaggtaaatt taatttttta gctatcggta tcggctttgc ctcgcacgta atcgcaaaca
      901 tgctatacat gaaattgact aattatcatt ctaaacaagc agaggcgcaa tgaccgcacc
      961 gaagttcgat aacaagatta gctggggaaa tattatttat cttgtaggtc ttatctttgc
     1021 cgtcgccgtg ttctacgcga aaacaacggc taacgataag acgcttgaaa aaattacgcc
     1081 ggaggttgag aatttaaaaa cagaaacgga agtattaaaa acggaactta aaagtataaa
     1141 caaaaaactt gacgagcaaa acgacttatt gcgcgagctt gtatttggaa agaacaggta
     1201 acaatgataa actttattat taaatttcaa aacgctattg gtcatattat cggcatcgtt
     1261 gcaacggctt tgataatcat gcaggtcccg gtgtatatgc cagcgtattt atatttttcg
     1321 cttgtagctt tcgttgtatg gattatgttt gaacttgtgc aagcggaggc ggcaatacga
     1381 agtgattatt ttaagaacag aaagaccgcg tcgttgttta cgtgtatatt gcgctatcaa
     1441 tggaaagaaa gtttattcaa ggatgtatta ggcaagaatt tatacgggct tggcttgtct
     1501 ataatcgttc ttggatttat tacgggctgg aaataatgac cgaacaaatt cgcaacataa
     1561 acaatacgcg cctattaagc gttgacgaaa gcatacgcga tcaagtgatg atgtgccttg
     1621 atagaatgca cgaacttgaa cagcccgccg tcattcatcg tgatgtatgg cggtctcctg
     1681 cattgcaaga gcttaagttc cgtcaaggcg tttctaagct aaaatggggc tatcattgcg
     1741 ccacacgtaa cggaaaaccc gcttccttag cttgcgatat tgtacacata gatcacgctt
     1801 ggttttctaa atttgacgct acatacaaag ccgatgagta cttttggctg tgcttaggtc
     1861 gttccgctaa agaggctgga cttgactggg gcggcgaatg gatgtggacc tggccatcaa
     1921 aagaggcgac ggaaaaggta cgtaaactaa acgaacgcat tgatacgctt gacgctacgg
     1981 ggcttgaaac agcgcgtgac ttcggctggg acgtagcgca tatcgaagtg cgcggtgtaa
     2041 gcgcagagga ggcggcgcaa ggttggcgca taagcaatgg tgttaagttt aagatagtac
     2101 cgtgattatg atatagtagt tatgaccaat gctgggcgat caatagcctt gtttgtttgt
     2161 ctgaaagaaa aggcttttta ttatcatggt aatcaggctt aaatgcttcg gcaagccatt
     2221 tagatttaac aaataaaatc acctgcgcat ataacactcg cacatgccgt gttgattatc
     2281 aaagtaaatt ccgctcttac cttcagttat ttcagggttc aaacacaccc atccacaact
     2341 attcattata ctacctttac caaatccatt attcatagga tgtctgcgta attctatttg
     2401 atgtttacaa ttacaacaac atgaaccatc atcccatcca taagaacatt ttgtcgcgcc
     2461 taacgtagta tttatggcat cggagggggg tgctttgtag cattggtcat ccggttgtgt
     2521 cgtttctact ccgcaatatt tacaagtgtg gagtaaacta tcaaataatg cgccattttc
     2581 gattcccatt tttctttcat ccttccaaaa tatatgaggt gtgccaatct gcaagcgttt
     2641 gtttggtatg tttactttta tgatacatat acgatagtta tgggcaatgc tactgaccgc
     2701 cattcaaacg agcttttgtt atttcaatag cctttggatt aatgtcgcaa cctataaaat
     2761 ttcggtttag ttctttgcaa actactgcgg ttgtgccact acctaaataa taatcagcaa
     2821 cgatatcgcc ttcatttgta aaagctaaaa taatcctttg tactaattct tttggttttt
     2881 cagttggata aagcagttta ctacctctca tttttatatc agtccaaacg cttcctatga
     2941 gctgttcatc tactaatatt ggtggtatgt ctttttggtg aacagcctta ccgttttcaa
     3001 atttttgaaa ccactttttc ctgggtttta ttttttcttt ctgctcgtta aaagtgtggt
     3061 tacttgattt cgcataaacc aatatcacat cgtggttttg tataaacttt ttgctttttg
     3121 cttttaaacc aagtctatat ttccaaataa tctcgtttct aaaattttca taaccaaaaa
     3181 cgtcgtcaat taaaattctc atccaatggt ttattctcca atccaactga aaaaccaaaa
     3241 ctccgttttg ttttagcacc cgtttcattt caataagtct tggtaggtag tggctttcaa
     3301 tttcgcttcg tataggtttt aagtcctgat aatcgccaaa gttcctacct gtgccataaa
     3361 gtatatcgca ataaatcata tttacgcttt cattcggctg tgatttta
//
LOCUS       DAOQGF010000214         3378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_16268_length_3378_cov_6.906109, whole genome shotgun sequence.
ACCESSION   DAOQGF010000214 DAOQGF010000000
VERSION     DAOQGF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16268_length_3378_cov_6.906109"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="xth"
                     /locus_tag="PK937_15915"
     CDS             complement(<1..609)
                     /gene="xth"
                     /locus_tag="PK937_15915"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNH34109.1"
                     /translation="MRLISWNVNGIRAVSKKGFLEWLDKESPDVMCLQETKAMPEQLD
                     ASLLNPMGYTGYFNSAQRKGYSGVATFCLKKPTSVQNGIGVERFDAEGRVLMTDHGDF
                     ILFNIYFPNGKMNAERLQYKMDFYDTVLKYFDALKDQGKKLVICGDYNTAHKPIDLAR
                     PRENEKISGFLPSERAWMDELVSHGYVDTLRVFNQEPGQYSWW"
     gene            878..1903
                     /locus_tag="PK937_15920"
     CDS             878..1903
                     /locus_tag="PK937_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH34110.1"
                     /translation="MIKFANPEYLYALAIIPLLALLLWDWFRTQRLRMLRVGDTRLVD
                     HLSQRIDPKRRLARWTMWTAGMIFGIFAMAGLSVGSRYEKVKITGVDMVIALDISASM
                     GAEDIKPNRLEKAKHEIGTLLSRLGGDRVALVIFAGQAFIQCPMTSDYSAVRMFLDAI
                     DVQSTTAGGTNFAEMLEIARSAFPKPQDVQGPATGKAIVIFSDGEDHSDEATEKAKAL
                     LGENIRIFAVGVATENAVPIPVYDDRTGQTDFKKFNGSVVTTKREDAMLTAITDMSGG
                     AYYQATINETEIQKIYDAISQLQKGESAEYQFTEYENQFQWFVGLSLIAVVFYIGIGN
                     RKTESRA"
     gene            1900..2886
                     /locus_tag="PK937_15925"
     CDS             1900..2886
                     /locus_tag="PK937_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNH34111.1"
                     /translation="MIEFRDPYFLLLLLLIPILIFWNWKRRRRSALRYATVALIRDVP
                     RSWRLRIKPLHYILQYAALGLLVITLARPRLQDTQSVITAEGIDIMMAMDISRSMLIE
                     DLGKVNRLEAAKSVAEKFVSGRQSDRIGLVLFAGKSFTQCPLTVDYTMLTQLIKQVQI
                     GVVEDGTAIGMGLVNSINRLRESKAKSKVIILLTDGDNNRGEIDPLTAAQIAQAMNIR
                     VYTIGAGKDGIARIPVDDPFFGRQYLTAEVKINETPLREIADMTGGKFFRATNQKALE
                     EIYDQIGQLEKTKVDVKTYQRYRELYLWFLIPALVCMLTVVVLSRTVFFSLP"
     gene            2883..>3378
                     /gene="tilS"
                     /locus_tag="PK937_15930"
     CDS             2883..>3378
                     /gene="tilS"
                     /locus_tag="PK937_15930"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNH34112.1"
                     /translation="MKTIEFTAWFEQRFFQNIHTKKLISKNHHILLAVSGGVDSMVML
                     RLFRRFAPELKLQLAIAHVNHGLRGKSAQDDAAFVKAIARLYGMPFYETRVHAKQHAR
                     RNKLSVEEACRVLRYDFLENTASTYGFDHVATAHHADDQAETVLMRIIKGTGIQGLSG
                     IPEVR"
BASE COUNT          899 a          830 c          766 g          883 t
ORIGIN      
        1 ccaccacgaa tattgaccgg gctcttgatt gaaaacacgc aaggtatcta cataaccgtg
       61 gctaaccaat tcatccatcc aggctcgttc agacggtaaa aaaccggaaa ttttttcgtt
      121 ctcacgcggg cgggccagat caatcggttt atgggccgta ttataatccc cgcaaatgac
      181 taattttttt ccctgatctt tcaacgcatc aaaatacttt aacacggtgt cataaaaatc
      241 cattttgtat tgcaaacgct ccgcattcat tttcccgttg gggaaataaa tgttaaacaa
      301 aataaaatcg ccgtgatccg tcatcagtac acgaccttcg gcatcaaatc gttctacgcc
      361 gataccattt tgaacagacg tcggtttttt taaacaaaat gtagccaccc cgctataccc
      421 tttgcgttgt gcggaattaa aatatccggt gtatcccatc ggattaagta gagatgcatc
      481 cagttgctcc ggcattgctt tggtttcctg taaacacatc acatccgggc tttctttatc
      541 cagccactca agaaaacctt ttttagatac ggcacgaatg ccattgacat tccaactgat
      601 aaggcgcata cgattcctct attaaaattg tatctctgtg ctttttattt atagattttt
      661 gtcaacatgc taagcgaccg attctagcat ctcttgcggc atcgctccac acatgggatg
      721 ggtacataaa acgcattgct gcggcgcgaa gaagataaaa attgtgttta taatttcaaa
      781 taaaaaatta aacacgcttt gattttgcaa aattgacttc atccgtatat attgaaaccg
      841 cttttaattt ttagtttaat tttatccgac ggacactgtg atcaaatttg ccaatccgga
      901 atatctttat gcacttgcga tcataccgct cctagccttg ctgctttggg attggtttcg
      961 cacacagcgc ctccgcatgc tgcgtgttgg cgatacccgc ttggtagatc acttgtctca
     1021 gcgaattgat ccaaaacgac gtttagcgcg atggacgatg tggacggcgg gaatgatttt
     1081 cggaattttc gctatggccg gtttatcggt tggcagtcgc tatgagaaag ttaaaataac
     1141 cggtgtggat atggttatcg cactggatat ctccgcgtcc atgggagctg aagacatcaa
     1201 acccaatcgt ttggaaaaag ccaagcatga aatcggcacc ctgctctcgc ggctcggcgg
     1261 agatcgtgtg gctttagtga tatttgcagg acaggctttt attcagtgcc cgatgaccag
     1321 cgactacagc gcggtgcgca tgtttctgga cgcaattgac gtgcaatcca cgacggccgg
     1381 tggtacaaat ttcgccgaaa tgctcgaaat tgcgcgatcc gcttttccga aaccgcagga
     1441 cgtgcaagga ccggctacgg gaaaagccat cgttattttt tcagacggtg aagatcacag
     1501 cgacgaggcc actgaaaaag ccaaagccct gctcggcgaa aacattcgca tatttgccgt
     1561 cggtgtagcc actgaaaatg cagttcccat ccctgtgtac gatgaccgca ccggccaaac
     1621 ggattttaaa aaattcaacg gatccgtcgt tacgaccaaa cgtgaagatg cgatgctcac
     1681 ggctatcaca gatatgagcg gcggcgcgta ttatcaagcc actatcaacg aaacagaaat
     1741 ccagaaaata tacgacgcca tctcccagct tcaaaaaggc gaaagtgccg aatatcaatt
     1801 cacagagtat gagaaccaat ttcagtggtt tgtcggtttg agtctgatcg ccgttgtatt
     1861 ttatatcggc atcggtaatc gcaaaacaga aagccgcgca tgatcgaatt tcgcgacccc
     1921 tattttttac tgctgttgct tttgattccg attctgattt tttggaactg gaagcgacgt
     1981 cgccgttcgg cgctgcgtta tgcgacggtc gcgcttatcc gtgacgtccc ccgctcctgg
     2041 cggctacgca tcaagccgct gcactacatt ctccaatatg cggcgctcgg tttactggtt
     2101 atcactttgg cgcgaccacg tttgcaggat acgcaatcgg tcatcacggc ggaaggcatt
     2161 gacatcatga tggccatgga catctctcgc agcatgctga tcgaagattt gggcaaggtg
     2221 aaccgcttgg aagcggccaa atcggtagcg gaaaaatttg tctccggacg tcaatccgat
     2281 cgcatcgggc tggtactttt tgcgggaaaa agttttaccc aatgccctct gacggtagac
     2341 tataccatgc tcacccagct tatcaaacaa gttcagatcg gcgtggtcga agacggcacg
     2401 gccatcggca tggggctggt taattccatt aaccgattaa gagaaagtaa agccaaaagt
     2461 aaagtgatca tattgctgac ggacggcgat aataaccgcg gtgaaattga tcctttgacc
     2521 gccgcacaaa ttgcccaagc catgaatatt cgtgtctata cgatcggcgc cggaaaagac
     2581 ggcattgccc gcatccctgt ggacgatcct ttttttggtc gtcaatacct gaccgccgaa
     2641 gtcaaaatca atgaaacccc gctgcgcgaa atcgccgata tgaccggagg aaaatttttt
     2701 cgtgctacca atcaaaaagc gcttgaggag atttacgatc agatcggcca attagaaaaa
     2761 accaaagtgg atgtcaaaac gtaccagcgt taccgtgagc tctacctttg gtttctgata
     2821 cctgcgctcg tatgcatgtt aaccgtagtc gtgctgtcac gtacggtttt ctttagcctg
     2881 ccatgaaaac catcgaattt accgcatggt ttgaacagcg tttttttcaa aacattcaca
     2941 ccaaaaaatt aatttccaaa aaccatcata ttttattggc tgtttccggc ggcgtagatt
     3001 cgatggttat gctgcgctta tttcgccgtt ttgcaccgga actaaaactg caacttgcca
     3061 tcgcgcatgt aaaccacggt cttcgcggaa aatctgcaca agacgatgcg gcctttgtca
     3121 aagcaatagc ccggctgtat ggtatgcctt tttatgaaac ccgcgtccat gcaaagcaac
     3181 acgcacgccg caataaactg tccgtcgagg aagcctgccg ggtgcttcga tatgattttt
     3241 tagaaaacac cgcgagcaca tacggttttg atcacgtcgc gacggcccac catgccgatg
     3301 atcaggccga aaccgtattg atgcgtatta tcaaaggaac aggcatccaa ggcttatccg
     3361 gaataccgga agtgcgcg
//
LOCUS       DAOQGF010000215         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_16504_length_3347_cov_6.287971, whole genome shotgun sequence.
ACCESSION   DAOQGF010000215 DAOQGF010000000
VERSION     DAOQGF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16504_length_3347_cov_6.287971"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>3347
                     /locus_tag="PK937_15935"
     CDS             151..>3347
                     /locus_tag="PK937_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34113.1"
                     /translation="MTENVTINVGNGSGPDSSAGIIVAPNLAAELPTPTTLNLFDLDG
                     TGNKMYVYSDGHVQVGGGNKSNMRLNGNANGLAGGPKLHMFGGKINVTGSLSLTTGDS
                     VYVQGGTLNIGLVASSGTNTITFAANDPGDTTAFIVDGGSVYVGDGNSAITMQTDNDN
                     IPSFDTGAVDAYNKLEIKNGIFYLNGRFQMADATARFDMSGGNFYVNPTGSQDLTGTN
                     SILLFQRGVVNFTGGTITLVNPNAYSGTGVALQVNGQATVGGTTVAANQLSGLPTALN
                     EVTDVVFQGTTSFGDGSASEDGSGDGFDITLADFGSSNPYGKFLVNSPSGSNRHVSLT
                     LQNSGGSHDSVLTVNDSLVIRAGEFRQNRNRIDYDGSGVAGMEIGYAGILRISSVQNF
                     DYFPQGFNSYVLDTVGSTVIYDGASSGTTVVDAGGSATFGNLTIAGNGTKQMNSSQRV
                     RGTLLLTSATLAAGSNLTMASGSTIKRDAGAMTASNNLGGNDYTIEYIGTNKNTAAGE
                     WSGTGKKHLKVNMTSGYTLTMHANLPTTGRIIDSLVIESGSTLADGGFTANVSGNLRV
                     NGTHSGTGFIRLDSATTHTITGNGSGTINRLSIFGVSGTDVTVTAASDFIINDSLLFE
                     ASAATDDRLLHIGANTLTMGSKMTPFFTNVSASSPARYITTNGSTSDDGIVKPYGSTG
                     SFTWPFGVSGKYTPATIDLTTAAVLGNVTLKPINSAHPVTTDPTNLELAYYWIASTTI
                     ASPVVNSKFAYVDADQSGRGTESAYIPAKYAPTTWTRINDVTAVSDGNATDTIYFNGV
                     NYITGDYTAGEPADFGSVLTYYSRQTGSWTDPNTWSTTGVGGSAAGTTPGSSRPVVIG
                     SGHIVYVNNNNLTSASMEIQSGAQLDINPGITGSNFGVSSGTGTLILRSSTSTPPTFP
                     SGDWSSFLGSSGGTVAYSGVGSYTLPSSPTTYNNLTITNSGAGNRTMPNTTLSISGNM
                     TLNAAQTVYFDSSASGNVTIGGDLRVSHASAILQLRGSLNARTISVSGDVENSGTISI
                     GSSTGSLTHALSIGDSLINNGTFNMVNGTNVCNVT"
BASE COUNT          865 a          692 c          824 g          966 t
ORIGIN      
        1 tttgggcgaa atgtaaccac tgttaacgca accggcggta atgtaacgtt ttacatgcga
       61 ggtaattctc tcttagatat tcaagaaggc tatttggtat tgtgcggcaa tccagcgctg
      121 acatctataa cggcaggcaa cttgtttaat atgacggaaa atgtaactat taatgtcggt
      181 aatggttctg gtcccgatag ttctgcgggt attattgtag cgcctaatct ggcggctgag
      241 ttaccaacgc ctactacatt gaatttattt gatctggatg gtacgggaaa taagatgtac
      301 gtgtattctg acggacatgt gcaggttggg ggtggtaata agagtaatat gcgcctcaat
      361 ggcaatgcca atggtcttgc cgggggaccc aaactccaca tgtttggcgg taaaataaat
      421 gtcaccggct ctttatcgct tacgactgga gattcagtgt atgttcaagg tggcacactg
      481 aatatcgggc ttgttgctag tagcggtaca aatacgatca cgtttgcggc aaatgatcct
      541 ggcgatacga cggcatttat tgtggatggc ggttcagtct atgtcggtga tggtaattca
      601 gctattacta tgcagacaga taatgataat attccgagct ttgatacagg ggcagttgat
      661 gcatataaca agcttgaaat caaaaacgga atcttttatc tcaatggtcg tttccaaatg
      721 gcggacgcta ccgcgcgttt cgatatgagt ggcggtaatt tttatgtaaa tccaaccggt
      781 tcgcaggatt tgaccggtac gaattcaatt cttttattcc aacgtggcgt ggttaatttt
      841 acgggcggta caatcacgct tgtaaatcca aatgcttatt cgggtaccgg cgtggcgttg
      901 caggttaatg gacaagccac ggtcggaggg acaacggttg ctgcgaatca gctttccggt
      961 ttacccacag cgcttaatga agtgactgat gtagtgtttc aaggtacgac atcattcggt
     1021 gatggtagcg catccgaaga cggttccggc gatggttttg atattaccct tgctgatttt
     1081 ggaagctcca atccttatgg aaaattttta gtaaatagtc ctagtggcag taatcgccac
     1141 gtgtcattga cgcttcagaa ttccggcggt tcgcatgact cggtactcac agttaacgat
     1201 agcttggtta tacgtgccgg tgaatttcgt cagaatagaa atcgtattga ctatgacgga
     1261 tcaggtgtag ccggtatgga aattggttac gccggtatat tgcggattag tagtgtccaa
     1321 aattttgatt attttcctca aggtttcaac tcttatgtgc ttgatacggt aggtagtacg
     1381 gtgatatatg acggcgcaag ctccggtacg accgttgttg atgccggtgg gtccgcaacg
     1441 tttgggaatc ttacaattgc aggtaatgga accaaacaaa tgaattcgtc tcaaagggtc
     1501 cgcgggacac ttcttttgac ttcggcgacc ttggctgctg gttccaatct gactatggcc
     1561 agtggttcaa cgattaagcg tgatgcgggt gctatgaccg cttcaaataa cctcggcgga
     1621 aatgattata ctatcgaata tataggtaca aacaaaaaca cggctgcggg tgaatggtcc
     1681 ggtacaggta aaaaacatct taaagtcaat atgacaagtg gatatacgct gaccatgcat
     1741 gccaatttac ctacgacagg tcgtattata gatagtttgg ttattgagtc gggatctacg
     1801 ttggcagacg gtggttttac tgctaatgtt tccggtaact tacgagtaaa cggtacacat
     1861 agcggtacgg gctttattcg cttggatagt gccacgacac acacgattac cggcaatggt
     1921 tcgggaacaa tcaaccgtct ttcgatattt ggcgtttcag gaacggatgt tactgtgact
     1981 gccgcgtccg attttataat caatgattca ttactttttg aagcttctgc tgctacggat
     2041 gatcgtcttt tgcacattgg cgccaatacc cttacgatgg gcagcaaaat gacgccgttc
     2101 tttaccaatg tcagtgcatc ctcacctgcg cgctatatta ctaccaatgg tagcacgtcg
     2161 gatgatggaa tagttaaacc gtacggtagt acaggttcat ttacatggcc ttttggtgta
     2221 tccggtaaat atacgccggc aaccattgat ttgacgacag cggccgtact tggtaacgtg
     2281 actttaaagc cgatcaatag tgcgcatccc gtaacgacag accctacaaa cttagaattg
     2341 gcttattact ggattgcatc tacgaccatt gcttccccgg ttgtgaactc caagttcgct
     2401 tacgtggatg ccgatcagtc cggccgtgga acggaaagtg catatattcc agctaagtac
     2461 gcaccaacaa cgtggacgcg tatcaatgac gtaactgccg tatctgatgg taatgctaca
     2521 gacaccatat attttaatgg cgttaattac attacaggtg actacacagc gggtgaaccg
     2581 gctgatttcg gatcggtttt gacttattat tcgcgtcaga caggtagctg gacggatcct
     2641 aatacatggt cgacaacagg tgtaggtggc tctgctgcag gaactactcc aggctcaagc
     2701 cgacctgttg tgattggttc tggtcatatc gtctatgtaa ataataataa ccttacttct
     2761 gcaagtatgg aaatacagtc tggagcacag ttggatatca accctggaat tactggatct
     2821 aattttggtg tttcatcagg tacgggtacg ctgatattgc gttcgagtac atcgacgcca
     2881 cctacattcc cgtctggtga ttggagttca tttttgggtt cttcaggtgg aacggttgct
     2941 tattcaggtg ttggatcgta tacgttacca tcaagcccaa caacttacaa taatcttaca
     3001 ataacaaatt cgggggccgg taatcgtacg atgccgaata cgacattatc aatttcagga
     3061 aatatgacgc ttaatgcagc tcagacggta tactttgata gtagcgcatc gggtaatgtt
     3121 acgatcggcg gtgacctacg cgtgtcccat gcatcggcca ttctccagct ccgaggttca
     3181 ttaaacgcca gaacgatatc tgtatccggc gatgtcgaaa attcaggtac gattagtatc
     3241 ggttcatcta ccggaagcct cacgcatgct ttatccattg gagatagttt gatcaataat
     3301 ggtacattta acatggtcaa tggtaccaat gtttgcaatg ttacttt
//
LOCUS       DAOQGF010000216         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_17099_length_3271_cov_5.985075, whole genome shotgun sequence.
ACCESSION   DAOQGF010000216 DAOQGF010000000
VERSION     DAOQGF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17099_length_3271_cov_5.985075"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK937_15940"
     CDS             <1..433
                     /locus_tag="PK937_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNH34114.1"
                     /translation="SFVDPERIGIWGRSGGGTFTLLAMTRTSLFKAGVAVAPLTSWHY
                     YDTKYGEATMKTPQENPDGYLKTDLNRYAKNLSGRLLLVHGTYDDNVHPQNTWHFVDE
                     LIRADKRFDMMMYPMRKHTFTDTAAKLHLCKTMIEFWKRNL"
     gene            441..1409
                     /locus_tag="PK937_15945"
     CDS             441..1409
                     /locus_tag="PK937_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH34115.1"
                     /translation="MPLIETSTYRAPLLLFNGHVQTIFPTLFRKVHDVSFTRERITTP
                     DDDFLDLDWLATHTSSKLVILSHGLEGNTSRAYVRGMAKRFHEEGWNILAWNYRGCSG
                     ESNRLLRSYHSGATEDLEVVIHHGLSRGYTEIVLIGFSLGGNMTLKYLGEKGNQVHPA
                     IRKAVTFSVPVDLKSSALTMESPACRIYMRRFLRMLHEKIKLKSTMFPGQISDTGFSQ
                     IKTFRQFDDRYTAPLHGFRDAEDYWSRCSAVRFLDNIAIPTLLVNAQNDPFLGPSCFP
                     ITQVQHHTHITLEMPRTGGHVGFPASSKKGYYWSEERALLFTNEGV"
     gene            1406..>3271
                     /locus_tag="PK937_15950"
     CDS             1406..>3271
                     /locus_tag="PK937_15950"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH34116.1"
                     /translation="MSKLSFQIFIFGFIITFTLQSQTENDSFKAAQSHATAQTYLKGL
                     KYDSAIVFLKDALKLYIKTENWKKAGTVYDEITYGQRISGFFMDATATATEGLALVKS
                     KLGDSNIVYGELLNTYATVLWSRGLYDSSLNINRRNLKLRTALLGPDHQDVGAVHTNI
                     GLAYKDKGYYEEALSHYEKGLKIRIKALGENHSSIASSYINMGVIYRIRGDYKTAEEL
                     YLKALNIRIKTIGEKHPRTAIIYFNIGICYDAMGDYERAMMFYRKAIDVALSTLGPEH
                     PDVSDMYIGIAICYYNQGMFHESLEYEKKALVIRLKRHDPSSVILAINYNNIGNVFRE
                     LKQIDSAEYYYQLAFTINKREYGSHSEVAKGYVYFGDIAERREHWREADSLFTLGLNM
                     AKDVLGFKNPLVAEILSRHASLAKKTNDITAAIRLSQAALNASLIGFNDSNILSNPTL
                     KDATLESVSIEILAQKARLWHQLFLTKHDVRHMEESFKLFELVSGWIDYLRQGYRLEG
                     SKIFLGTKAKRIYEEGIRACVTAYRATQAPLWIERAFTLSQRSKSGALREALHDLQIR
                     NFTQMPDSVSNLERDIKINLASYETQLQNQLQLHDSSKHSEIQRLERILFYQKTQY"
BASE COUNT         1008 a          676 c          683 g          904 t
ORIGIN      
        1 atcatttgtt gaccccgaac gtattggcat atggggtcgc agtggcggcg gaacctttac
       61 tctactggcc atgacacgaa cgtcgctttt taaggccggt gtcgccgtcg ctccgctcac
      121 tagttggcat tattatgata ccaaatatgg tgaagctaca atgaagaccc ctcaggaaaa
      181 tccggatggt tatcttaaaa cagatctcaa ccggtatgcg aaaaacctga gcggaagatt
      241 actcctagtg catggcacgt atgacgataa tgtccatccg caaaatacat ggcactttgt
      301 tgacgagctg attcgcgcgg ataaacgttt tgacatgatg atgtacccca tgcgcaaaca
      361 tacgtttacg gatacagcgg ccaaactgca tctatgcaaa accatgattg aattttggaa
      421 acgtaattta tagaaggact atgcctttaa tcgaaacatc aacataccgg gcgccattac
      481 ttctttttaa cggccatgtg caaacgattt ttccgacgtt atttcggaaa gtacatgacg
      541 tttcatttac gcgtgaacgc ataacgacac cggatgatga ttttctggat ttggattggc
      601 tagctacgca cacatcatcc aaactagtaa tcctatcgca tggtcttgag ggaaatacct
      661 cgcgcgctta cgtgcgcggc atggccaaac gatttcatga agaaggttgg aatattctcg
      721 cctggaatta tcgcggatgc agcggtgaat cgaatcgttt gctgcgttca taccacagcg
      781 gagccaccga agatttagaa gtcgtcatac atcatggact cagccgagga tatacggaaa
      841 ttgtattgat cggatttagt ttgggtggaa acatgaccct caaatatctc ggtgaaaagg
      901 gaaatcaagt ccatccggct atccgtaaag cggtcacatt ttcagtccct gtggacctca
      961 agtcatcggc attaacaatg gagtctcctg cctgccgcat ctacatgcgt cgttttttgc
     1021 gcatgcttca tgaaaaaata aaattgaaat caacgatgtt tcccggacaa atcagtgaca
     1081 caggattttc acaaattaaa acatttcgcc aatttgatga ccgctatacg gcaccgttac
     1141 atggttttcg tgacgctgaa gattattggt cccggtgcag tgccgtgagg tttttggata
     1201 acattgcgat accgacatta ctggtcaatg cacaaaacga tccgttttta ggcccgtcgt
     1261 gctttcctat cacacaggtg cagcatcaca ctcacattac cttagaaatg cctcggacgg
     1321 gaggacatgt cggttttcct gcatcttcta aaaaaggtta ttattggtcg gaagaaagag
     1381 cgctactatt tacaaatgag ggggtatgag taaattaagt tttcaaatat ttatttttgg
     1441 attcattata acttttacgt tacagtcgca aacggaaaat gattcattta aggcagccca
     1501 gagtcacgcc acagcacaaa cctaccttaa agggcttaaa tatgatagcg ctatcgtttt
     1561 cctcaaagac gctttaaaac tctatataaa aacagaaaat tggaaaaaag ctggaactgt
     1621 atatgatgag atcacttatg ggcaacgtat aagcggattc ttcatggacg ctacagctac
     1681 agctacagaa ggtcttgcac tcgttaaaag taaattaggc gattcaaata ttgtttatgg
     1741 agagctactc aatacatatg ccactgtttt gtggagccga ggcctttatg attcatcatt
     1801 aaatatcaat cgtcgaaact tgaaacttcg gacagcatta ttgggacctg atcatcagga
     1861 tgttggggcg gttcatacta atatagggct tgcttacaaa gataaaggat attatgaaga
     1921 agcgctctct cattatgaaa aaggcctcaa aattcgaata aaagcgctgg gcgaaaatca
     1981 ttcttcgata gcgtcttcgt acattaacat gggggttatt tatcgcatac gcggagatta
     2041 taaaactgcg gaagagttat atttaaaggc tttaaatata cgcattaaaa caatcggtga
     2101 aaaacatccg cgaacggcta ttatatattt taatattgga atttgttatg acgctatggg
     2161 cgattacgag cgtgcaatga tgttttatcg taaagccatt gatgtcgcac tcagcacgct
     2221 aggaccagaa cacccagatg tatcggacat gtatattggc attgcaattt gctattataa
     2281 tcaaggtatg ttccatgaat ctctcgaata tgaaaaaaag gctcttgtaa tacgcctaaa
     2341 acgtcatgat ccatccagtg ttatccttgc aataaattat aacaacattg gaaatgtttt
     2401 cagagaatta aaacaaattg attcagccga atattactat caactagctt ttacgattaa
     2461 caagcgtgaa tacggatcgc actctgaggt cgcaaaagga tatgtttatt tcggagatat
     2521 tgccgaaagg agagaacatt ggagagaagc tgattcttta tttactctgg gattgaatat
     2581 ggccaaggat gtacttggct ttaaaaatcc attggtggca gaaattttat ctcgccacgc
     2641 atccttagca aaaaaaacta acgacatcac agcggctatt cgtttaagcc aagcagcgtt
     2701 aaatgcctct cttattggat ttaacgatag taatattcta tccaatccta cgttaaaaga
     2761 tgcaacacta gaaagtgtaa gcattgaaat tttagcacaa aaagctcggc tatggcatca
     2821 actttttcta acaaagcacg atgttcgaca tatggaagag tcgttcaaac tattcgagtt
     2881 ggtatcgggg tggattgact atctccgtca aggataccgc ctcgaagggt caaaaatttt
     2941 tctaggcacc aaggctaaac gaatttatga agaaggtatc cgggcttgcg ttacagccta
     3001 ccgtgcaacg caagcaccgt tgtggattga acgcgccttt acactgtccc aacgcagcaa
     3061 atcaggtgct ttgcgcgaag ctttgcatga tcttcaaatt cgtaatttca cacaaatgcc
     3121 cgattccgta agtaatctag aacgcgatat aaaaatcaac ttagcttcgt acgaaactca
     3181 actccaaaat caacttcaac ttcacgacag ttccaagcat agcgaaatac aacgccttga
     3241 gagaatttta ttctatcaaa aaactcaata c
//
LOCUS       DAOQGF010000217         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_17981_length_3168_cov_6.469965, whole genome shotgun sequence.
ACCESSION   DAOQGF010000217 DAOQGF010000000
VERSION     DAOQGF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17981_length_3168_cov_6.469965"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK937_15955"
     CDS             complement(<1..229)
                     /locus_tag="PK937_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34117.1"
                     /translation="MASLLVQSGRILLGRASISVISIFFTIYYARTLSRTQFAFIVLY
                     ETAAALFMSLTELGMYSKVVREAPPLLRDHKE"
     gene            complement(229..1389)
                     /locus_tag="PK937_15960"
     CDS             complement(229..1389)
                     /locus_tag="PK937_15960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNH34118.1"
                     /translation="MRVFILAMKYLPASGGTATYAHNLAQGLARMGHDVLLVSPDYAR
                     PQSDDGLPYRVIRLSQARLGLGQFRIFLTAYHTKKILNNFNPDVIWASTYAGCRVLGL
                     LRLRIPTVGTIHGGGIQRAFSSLNPIKRCTDHAGWRFMRRASAMITVSEEAKKLVCSA
                     IQDTLIQSKFRVIYNGIDFDPSSVVSKKEALSRWPQFEGRIILLTVGRLVKAKGHDVV
                     IRALPEIQKRVPNILYVIAGEGVEKEQLINLVESLSLKNSVHFTGYITPTGLEELYAI
                     SDLFVLAGRWTPTFVEGFGLVLVEAAIRGKPVIGARIGGIPEAIEDGVSGIVIEPEDP
                     SQVAKAVMDILSDQNLYDRMAKAGPVWVNLHFTQAIMARNIDQLLCYITGKS"
     gene            complement(1386..2588)
                     /locus_tag="PK937_15965"
     CDS             complement(1386..2588)
                     /locus_tag="PK937_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH34119.1"
                     /translation="MLERLLRQLHENNVTEARILISEDQSHHLSFRREFSSRYVMHLT
                     YIPTRETITQYLSKAKISSDVLLLNAHVLHDLRIIKKLVDSTNSLIIVDENHSGPCAA
                     KFIHDISIVYDNSGQDIELRKTLLILAQQKSIEVLRVSEMQSYVRFLRRHATPLFQSI
                     TPSTDMNALEHIMYEDTFKGGMEWIAVYGYKIPVRELTKWCARTSWITPNVITALATI
                     GKLVAIPFFFIGWIGWALLSFWIFTIGDSLDGKLARMTVRFSKTADRVDHLTSAPARM
                     LWHFGLTWYMCGGNLTSPIGMAGIGFSLLPQIDKFGLMYFNWKFGRSPIDFTSFDARV
                     HLYTARRNDMVLLTIGYLAGFLLPAFYGMMAWMYLTWLWHVVRISWFSFINPVTRGSV
                     FKKHTTHL"
     gene            complement(2645..>3168)
                     /locus_tag="PK937_15970"
     CDS             complement(2645..>3168)
                     /locus_tag="PK937_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNH34120.1"
                     /translation="DEVLIANIEKVTDIIISAFRSGHRLYLCGNGGSAADAQHIAAEL
                     SGRFYYDRKPLYAEALHVNSSYVTAVANDYGYDVVYERMVDAAGQAGDVLIGISTSGN
                     SPNVVKALQKAKVLNMHTVGMTGANGGVMKGICDILINIPSKDTPRIQECHILVGHIL
                     CELIEQTLFPKPK"
BASE COUNT          965 a          729 c          628 g          846 t
ORIGIN      
        1 actctttgtg gtctcgcaac agcgggggcg cttctcgaac tactttcgaa tacatgccta
       61 attcagtaag agacataaac aatgcagcgg cggtttcata aagaacaata aaagcaaact
      121 gagtacgtga cagtgtccta gcgtagtaga ttgtaaaaaa aattgagata accgaaatgc
      181 tggcacgacc aagtaatatg cgacctgatt gaactaataa actggccatc aggatttacc
      241 ggttatgtaa caaagcagtt gatcgatatt tcgtgccatg atagcttgtg taaaatgcag
      301 gttaacccac acagggcctg ctttcgccat tcgatcatac aaattttggt cactcagaat
      361 atccatcacc gcttttgcaa cttgagaagg atcttcgggt tctatcacta tcccggaaac
      421 gccgtcttca atggcttcgg gtattccgcc tatacgtgca ccgatgaccg gtttcccacg
      481 aatagccgct tcaaccagca caagcccgaa tccttcgaca aaagtcggag tccagcgacc
      541 agccaacaca aacaagtcac tgattgcata aagctcttcg agaccagtag gtgtaatata
      601 tcccgtaaaa tgaacactat ttttcaaact taacgattcg actaaattaa tgagttgttc
      661 tttttcgaca ccttcaccgg caatgacata taaaatatta ggaactcttt tttgaatttc
      721 tggaagtgca cggattacca catcgtgccc tttggcttta actaatcgcc cgacggtaag
      781 caaaataatg cgcccttcaa attgtggcca gcgtgagagc gcttcttttt tggataccac
      841 cgaagaagga tcaaaatcta taccattata aataacccga aatttacttt gtatcagtgt
      901 atcctggatg gccgaacaaa ctaatttctt tgcttcttca ctgacagtga tcatcgcaga
      961 cgcacgtcgc ataaaacgcc atcctgcatg atctgtacaa cgtttgatcg gattaagtga
     1021 tgaaaaagcc cgttgaatgc ctccgccatg aatagtccct acggtcggta ttcgcaatct
     1081 aagtaaccca agcacacgac aaccggcata tgtagatgcc caaatcacat ccggattaaa
     1141 attatttaga atcttctttg tatgataagc cgttagaaaa attcggaatt gacctagccc
     1201 aagtcttgct tgggataaac gtattactcg atacggtaat ccgtcatccg actggggtcg
     1261 cgcatagtca ggagatacca ataatacatc atgtcccata cgggcaaggc cttgtgcaag
     1321 attatgtgca taggttgcag tacctccgct cgctggaaga tacttcattg ccagaatgaa
     1381 aactctcata aatgggtggt atgcttttta aaaacagatc cgcgcgtgac gggattgata
     1441 aatgaaaacc atgaaatacg cacgacatgc cataaccatg tcagatacat ccaagccatc
     1501 attccgtaaa aagcagggag taaaaatcct gctaagtatc cgatcgtaag caacaccatg
     1561 tcatttcgtc gagctgtata aaggtgaacc ctagcatcaa acgatgtaaa atcaatcggt
     1621 gaacgtccga atttccaatt gaaatacatc aatccaaact tgtcaatctg tggcaagaga
     1681 ctaaatccaa taccggccat acctatagga gaggtgagat taccaccgca catataccac
     1741 gtcaaaccaa aatgccaaag catgcgggcc ggtgcactag tcagatggtc cactcggtcg
     1801 gctgttttac taaaacgaac ggtcatacgt gccagttttc cgtcgagcga atctcctatg
     1861 gtaaagatcc aaaaacttaa caaagcccaa ccgatccaac ctataaaaaa gaaaggaatc
     1921 gccaccaatt ttcctattgt agctaaagct gtaatgacat tgggtgttat ccatgaagtt
     1981 cgggcgcacc attttgtaag ttcccgcacc ggtatcttat atccatatac agcaatccac
     2041 tccatacctc ctttgaaggt gtcttcatac attatatgct ccagtgcatt catatcggtg
     2101 gatggcgtaa tgctttgaaa taacggcgta gcatgacgac gtaaaaaccg aacataagat
     2161 tgcatttcag aaactcgtag cacttctatg cttttctgct gagctagtat aagaagtgtt
     2221 tttcgcagtt caatgtcttg ccctgaatta tcgtacacaa tcgaaatatc atgaataaat
     2281 ttagcagcac atgggcccga atgattctca tcaacaatta tcaacgaatt ggtcgaatct
     2341 actagttttt ttatgattcg cagatcgtgc aatacgtgag cattcagtag aagtacatcc
     2401 gacgaaatct tggccttcga taaatactgt gtaattgttt cacgcgtagg aatataagtc
     2461 agatgcataa cataacgaga cgaaaactca cgccgaaacg aaaggtgatg agactgatct
     2521 tcagaaatca gaatccgtgc ttcagtaacg ttgttttcat ggagttgacg caataagcgt
     2581 tctagcatgg atatactata caaacagcgt ctggacgccg tatttcgcgc atctactata
     2641 gctatcattt gggttttgga aaaagtgttt gttcgatcaa ttcacacaga atatggccga
     2701 ctaatatatg acactcttga attcggggtg tatctttgga aggaatattg atcaaaatat
     2761 cacaaatacc tttcatgaca ccgccgtttg ctcctgtcat tccaacggta tgcatattta
     2821 gcacttttgc cttttgtaat gccttaacca cattaggaga attcccagaa gtggagattc
     2881 ctatcaaaac atcaccagcc tgccctgcgg catccaccat acgttcatac actacatcgt
     2941 atccgtagtc attggcaacc gctgtcacat aagacgaatt gacatggagt gcttccgcat
     3001 acaatggttt gcggtcataa taaaagcgtc cggataattc ggctgcaata tgttgtgcgt
     3061 ctgccgcgct gccgccgttt ccacaaagat acaaccgatg acctgagcga aaagccgata
     3121 tgataatatc ggttactttt tcgatgtttg cgatcaatac ctcatccg
//
LOCUS       DAOQGF010000218         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_18748_length_3087_cov_7.111148, whole genome shotgun sequence.
ACCESSION   DAOQGF010000218 DAOQGF010000000
VERSION     DAOQGF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18748_length_3087_cov_7.111148"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..1262
                     /locus_tag="PK937_15975"
     CDS             384..1262
                     /locus_tag="PK937_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH34121.1"
                     /translation="MKKLLSCLILFYLSVPSAEGSERTLPYFVSTQWLADHINDSDLV
                     ILQTAFTRKEYAVGHIPGSRFLWFNWLAMDTPDLSTEMPDSVTAKNVLENLGISQKSK
                     IVVVFTRGSVTTTTRMLVALSYFGFDNQTAVLDGGLDVWKAEGRPITKDIPTVQKTAL
                     ALKLHPEIITNAEWVKSRLSSEKVKIIDARSKNFYAGNGGGILRTGHIEGARNIPFST
                     LTDSTNKILPVASLQKIIDDAGIQKGDTVVTYCHVGQQATLVYTVARMLGYEVLLYDG
                     CFEDWNVRDESYPVKK"
     gene            1266..1778
                     /locus_tag="PK937_15980"
     CDS             1266..1778
                     /locus_tag="PK937_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH34122.1"
                     /translation="MKPMKPYMNPYVAGVGLGLVLLLAFFIVGRGLGASGALSSVLTS
                     TVNTVAPSHIQSNAFYSEYYNNGNPLKDWLVFEMAGVLIGGLLSGWFSGRLKFKVEKS
                     ESVSTCTRFIFAFAGGILMGLGSKLARGCTSGQALTGGALMSVGSWIFIASVFITAYL
                     VAKFFRRQWK"
     gene            1775..2362
                     /locus_tag="PK937_15985"
     CDS             1775..2362
                     /locus_tag="PK937_15985"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNH34123.1"
                     /translation="MNAPFLKFDMISFEQSLIIAVFIGIGFGFFLEQAGFGSAVKLAQ
                     QFYLNDLSVFKVMFSALVTAMLGLFWLNKLDLLDLSAIRVLPTYIYPQLAGGVVFGVG
                     FIMGGYCPGTCCVASVTGRMDGWIHFIGMMTGIFIFAEVYPFIESWYFSTSLGDITLF
                     QFFQIPYGTAVFIVVMMALLGFVAAERIEKLHAKD"
     gene            2367..2987
                     /locus_tag="PK937_15990"
     CDS             2367..2987
                     /locus_tag="PK937_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNH34124.1"
                     /translation="MTVNRFLFAICFVFGLLALLSGNSDPYHDKINEIADLVETENDH
                     VLPDSLAVWLMNEKPMRIIHLGDSSSYSRYHIPGAELKSLPDLLHSTLKKNELIILYS
                     EGGIHAGQAWVLLKAKNFKNVYTMHGGLSAWRSEILFPRLSPDAKDAEKKRFEERSKI
                     SLYFGGQPTVILEPKPEVTMKKQEPPRKKIDTPRFEKEEEKIRDGC"
BASE COUNT          830 a          599 c          720 g          938 t
ORIGIN      
        1 ggcgtgttcg cgaaaaattt ccacatctgc tttttgggta tatgaataaa ccggcggagg
       61 taattagaga cgctgtagat cgctcgactt aagcaggatg cacgaagata caagtttagt
      121 tcacaccttc atgatcgcgg acaaccgtct gccgaacgtt tttacgatat ataagtaata
      181 gcttagttgt aagcgtagca aatttcagtt tgcaaattta aatgcgcgat ggtttggtat
      241 tgttcttagc aatgaacgca tagatttgtc gggagtcacg cattataaaa aacatgaaac
      301 ataaaccaat taaaatcgta aagacttacg caagtctata ccgttcatct ccctattaaa
      361 tttataagag gtaatttagt tttatgaaaa agcttctttc ctgtttgatt cttttttatc
      421 tgtcggtccc gtccgcggaa ggttcagagc gtacattgcc ttattttgtt tcaacccagt
      481 ggttggccga tcatatcaat gattctgatc tagtgatact tcaaaccgct tttacacgaa
      541 aagagtacgc tgtagggcat attccggggt caagattttt atggtttaac tggctcgcta
      601 tggatacgcc ggatttatcc acggaaatgc cggatagtgt aacggctaaa aatgttttgg
      661 aaaatcttgg tatctctcaa aaatcaaaaa ttgtcgtcgt gtttaccaga ggtagtgtta
      721 ccaccacgac gcggatgctc gtcgcgcttt cttattttgg atttgataat cagacagcag
      781 ttctggatgg tggtcttgat gtatggaagg cggaagggcg tccgattacc aaggatatac
      841 cgaccgtaca aaaaacagct ctggcgctta aactccatcc ggaaatcatc accaatgcag
      901 aatgggtcaa gtcgcgtttg tcatccgaaa aagttaaaat catagatgcg cgttctaaaa
      961 atttttacgc cggcaatggc ggcgggatat tgcgaaccgg tcacattgaa ggcgccagaa
     1021 atattccctt ttccacattg acggatagta ccaataaaat tttacccgtc gccagtttgc
     1081 aaaaaatcat tgatgatgca ggcatccaaa aaggcgacac ggttgtcacc tactgtcatg
     1141 tcgggcaaca agccactttg gtatatacag ttgcacgtat gttagggtat gaagttttat
     1201 tatacgacgg atgttttgaa gactggaatg tacgtgatga atcgtatccg gtaaaaaaat
     1261 agaccatgaa accgatgaaa ccttatatga atccctatgt ggcgggtgtc ggacttgggt
     1321 tggttttact cttggccttt ttcattgtgg gacgaggtct gggtgcctcc ggtgcattga
     1381 gttctgtttt gacttcgacc gtaaatacgg tcgcaccatc gcatatccag tcgaacgctt
     1441 tttatagtga atattacaat aacggaaacc ctctcaaaga ctggttagta tttgagatgg
     1501 ccggcgttct gatcggcggc ttattatccg gttggttttc gggaagatta aaatttaagg
     1561 tcgaaaaatc agaaagcgta tccacctgca cgcgttttat ttttgctttt gccggcggaa
     1621 ttctgatggg tttgggttcc aagcttgctc gcggatgcac gagcggtcag gctttgacgg
     1681 gaggtgcatt gatgagtgta ggcagttgga tatttatcgc ttccgtgttt ataacggcgt
     1741 atctggtcgc taaatttttt agaaggcaat ggaaatgaac gccccttttt tgaaatttga
     1801 catgatcagt tttgaacaat cgctgataat agcggttttc atcggtattg gattcggttt
     1861 ttttctcgag caagccggtt ttgggagcgc cgtcaaacta gcgcagcaat tttatctaaa
     1921 tgatctctct gtattcaaag ttatgttttc tgcgcttgta acagctatgt tgggtttgtt
     1981 ttggttgaat aaactggatt tgctggatct ttcggctatt cgtgtgcttc ccacgtatat
     2041 atatccgcag ctcgccggag gcgtcgtttt tggtgtcgga tttattatgg gcggatattg
     2101 tccgggtacg tgttgtgtgg cttctgttac cggtcgaatg gatggatgga ttcattttat
     2161 cggaatgatg acaggaattt ttatttttgc ggaggtctat ccctttatcg agagctggta
     2221 tttttcaaca tcgctgggag atatcacctt gtttcagttt tttcaaatcc cgtacggcac
     2281 ggctgttttt atcgtagtta tgatggcgtt attaggcttt gtagccgctg aacgtattga
     2341 aaaattacat gcaaaggatt gaacgcgtga cggttaatcg ttttttattt gcaatctgtt
     2401 ttgtgttcgg tcttttagcg ctgctttcag gcaattcaga tccttatcat gacaagatca
     2461 atgaaatagc ggatcttgtc gaaacagaaa acgatcatgt tttgcccgat tcattagctg
     2521 tttggcttat gaatgaaaaa cccatgcgta tcattcatct tggtgattcg tcatcgtact
     2581 cccgatacca cattcccggt gctgagttaa agtcgcttcc tgatctgttg cattccacct
     2641 tgaaaaaaaa tgaattgata attctatatt ccgaaggcgg cattcatgcc gggcaagcat
     2701 gggtgctact caaagctaaa aattttaaaa atgtgtatac gatgcacgga ggattatcag
     2761 cctggcgttc ggaaatattg tttccaaggt tatcgcctga tgcaaaagat gcagagaaaa
     2821 aacgtttcga agaaagaagt aagataagtc tctactttgg cggccaacct acggtgatcc
     2881 tggaacctaa gcccgaagtg acgatgaaga agcaagaacc gccccgaaaa aaaatagata
     2941 ctccgcgatt tgaaaaagaa gaagaaaaaa tcagagacgg ttgttaaaac tattttccgc
     3001 tgctgagtac ccgaaacggc agcctaaata atgcccagat aaacttgagt acaccctcca
     3061 ccgccaatcc ggcaattcga aacggca
//
LOCUS       DAOQGF010000219         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_18905_length_3072_cov_3.487570, whole genome shotgun sequence.
ACCESSION   DAOQGF010000219 DAOQGF010000000
VERSION     DAOQGF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18905_length_3072_cov_3.487570"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..751)
                     /locus_tag="PK937_15995"
     CDS             complement(29..751)
                     /locus_tag="PK937_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH34125.1"
                     /translation="MKKIDTVSVIIPVFNEVNSIRSIIEAVRNAPLTINAQTLIKETI
                     IVDDCSTDGTRDLLENELQSLVTKTIYHTTNTGKGAALRTGFQHASGDIIIIQDADLE
                     YDPNEYIKLITPIIDNKADVVYGSRFAGSDAHRVLYYWHYLGNKFLTTLSNMFSNLNL
                     TDMETCYKVFRSEIIRNIRLEQNRFGFEPEVTAKIAKIKDIRIYEVGISYHGRTYAEG
                     KKINWKDGFSAIWCIIKYNLFK"
     gene            complement(738..1895)
                     /locus_tag="PK937_16000"
     CDS             complement(738..1895)
                     /locus_tag="PK937_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNH34126.1"
                     /translation="MRIGICALGAVSQDTGGQTYLKNFAKTLGEIKTNNEFVWFFSKG
                     ESKVIEFPDNTSTNVVVELPLSARTVQRLVCEHILLPLFSDHHRVDVMFFPNNYASIF
                     YRKPFVLAIRSMLIYNTKSHGVNLIRSFYRSIIAPLSAKKAAIIITPSYHTKLEIIKH
                     LNISDNNIEVISHGIDIQLFGSEKNSDAADTLWKKWNIKPPFILYVSALWSYKNHDKL
                     ILAFDKYRKSKKTDFQLVLIGKGASSFESYGKYLKDLITDLKLSEVVRFIDFLPHQDL
                     KHFYQWASVYAFPSQTESFGNSIYEAMAAGAPIICSNTHGYETKVKEAVLSVNPHDIN
                     CFADAINAVINNKNLRANLIKNGKIQANNMSWDNCIRNTVLIIEKANYEKN"
     gene            complement(1897..>3072)
                     /locus_tag="PK937_16005"
     CDS             complement(1897..>3072)
                     /locus_tag="PK937_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNH34127.1"
                     /translation="VTFGIVLSVVTLELLVRLAGAKPQTYLRKFSRYHTILGWEKTPL
                     TEGKFRKGDVIIHEKINSRGLRDSEYTYEKPSNTFRILVLGDSFTEGYDVEINDLFTE
                     ILESKLNTSSFDSSSIRYEVINAGTGGYSTDQEFLYFEIEGIKYKPDMVLLMVYPAND
                     IFYNHKSKYGNYNKPFFQIRNDSLILTNTPLQEPYRQESIKNLFREMALYPIVTQIIL
                     TKYPRISQTLSHFGFISESTLAATAANLNSISDSSASSFSVYKKYNNFFMNTAWQKTS
                     RIIYELNKKSKEIGTRLVMFYIPDRIEVYDSDWKKTKEKFSLEDHEWSPDVPSTMLKK
                     ISDTSNIPLLDFRQYISKKELGDSVLYNGIHWNVSGNRFAAEFIYEALFHEKATVKQ"
BASE COUNT          922 a          534 c          555 g         1061 t
ORIGIN      
        1 acccatgttt tcatccttat aaataagctt atttgaatag attatatttt attatgcacc
       61 aaatggctga aaaaccgtct ttccagttga ttttttttcc ttctgcatag gtgcgaccgt
      121 gatatgaaat gcccacttca taaatgcgaa tgtctttgat ttttgcgatt ttggcagtga
      181 cttccggttc aaagccaaat cgattttgtt ctaggcggat attcctaatg atttcgctgc
      241 gaaatacctt ataacatgtc tccatatcag tcaggttcag attagagaac atattggata
      301 atgtagttag aaatttatta cctaagtaat gccaataata caaaactcta tgtgcatctg
      361 atccggcaaa acgcgatcca taaacaacgt cagctttatt atcaattatc ggtgttatca
      421 gctttatata ttcgttagga tcatattcta aatcagcatc ttgtatgatt atgatatcac
      481 cacttgcgtg ttggaatccg gttcttaagg cggccccttt tcccgtgtta gtagtatggt
      541 agattgtttt agttacgagc gattgaagct cattttctag cagatcacgt gtgccatccg
      601 tcgaacaatc atcgactata atagtttctt ttattaaagt ttgtgcattt atggtcaatg
      661 gtgcattgcg tacggcttca ataatagatc gaatactatt tacttcatta aatacaggaa
      721 tgataacgct tactgtatca atttttttca taatttgctt tctcaataat taatacagta
      781 tttctgatgc aattgtccca tgacatatta ttggcttgta ttttaccgtt ctttattaag
      841 ttagccctta gatttttatt gtttatcacc gcattaatag cgtctgcaaa acaatttata
      901 tcatgtgggt ttacggatag gacggcttct tttacttttg tttcatatcc atgcgtatta
      961 gaacatatga tcggcgctcc agcagccatt gcttcgtata tcgaatttcc aaaactttct
     1021 gtctgagaag ggaatgcgta aacactagcc cactgataaa aatgcttcaa atcctgatgc
     1081 ggtaaaaaat caatgaatcg tacaacttca gatagtttga ggtcagttat taaatctttg
     1141 agatattttc cgtagctttc aaaactactg gctcccttgc caattaaaac tagttggaaa
     1201 tcggttttct ttgattttcg gtatttatca aatgctaaga tcagtttgtc atggttctta
     1261 taactccata aagctgaaac atataggata aaaggaggtt ttatattcca ttttttccat
     1321 agcgtatctg cggcgtcaga gtttttttca ctgccaaaaa gttgaatgtc tatgccgtgt
     1381 gaaattacct ctatgttatt atctgatatg ttgaggtgtt taatgatttc aagtttagta
     1441 tgataagaag gggtaattat gattgcggcc ttcttagcac ttaaaggagc tattatagat
     1501 cggtaaaatg agcgtatgag atttacgccg tgtgattttg tgttgtatat caacatactt
     1561 cgtattgcca agacaaaagg ctttcggtag aaaatagatg cgtaattgtt aggaaaaaac
     1621 ataacatcta ctcgatgatg atcggaaaac aatggcaaca aaatgtgttc gcatactaac
     1681 cgttgaacag ttctcgcgga aagtggtaat tcaactacca cattagttga agtattgtct
     1741 ggaaattcaa taacttttga ttcgccttta gaaaaaaacc aaacgaactc attattagtt
     1801 ttgatttcgc cgagcgtttt tgcaaaattt tttaaatagg tctgtccgcc tgtgtcttgt
     1861 gaaacagcgc ctaaagcgca aatacctata cgcatattat tgttttacag tcgctttctc
     1921 atgaaataat gcctcgtata tgaattctgc cgcaaaacga ttgcccgata cattccaatg
     1981 tattccgtta tataaaacag aatcgcctaa ctcttttttt gagatatatt gccgaaaatc
     2041 aagtaaaggt atattggatg tgtctgaaat ttttttaagc attgtcgatg ggacatcggg
     2101 actccattca tgatcttcaa gggagaattt ttctttggtt tttttccaat cggagtcata
     2161 aacttcaata cgatccggaa tataaaacat aaccaatcgc gtgccgattt cttttgattt
     2221 cttatttaat tcataaataa tgcgcgaagt tttctgccaa gcggtattca taaaaaaatt
     2281 attatatttt ttatataccg aaaatgatga tgctgaagaa tctgaaatag agtttaaatt
     2341 agcggcggta gcagccagtg ttgattcaga aataaatcca aaatgcgaca atgtttgtga
     2401 aattcgaggg tattttgtca aaatgatttg agtaacgatc ggatacaggg ccatttctct
     2461 aaaaagattt ttaatggatt cttgacggta tggttcctgt aacggagtat ttgttaatat
     2521 gagtgaatca ttcctaattt gaaaaaacgg tttattatag ttcccgtact tacttttatg
     2581 attgtaaaaa atgtcgtttg ctggataaac catgagtaaa accatatccg gtttatactt
     2641 gataccttca atttcgaaat acaggaactc ctgatccgtt gaatatccgc cagttcctgc
     2701 attgattact tcatatctaa ttgaagatga atcaaaactt gaagtgttta gttttgattc
     2761 aagaatttca gtaaataggt cattgatttc aacatcataa ccttcggtga atgaatcacc
     2821 gagaacaaga attcgaaaag tgtttgaagg tttctcataa gtgtactctg aatcgcgtaa
     2881 ccctcggcta ttaatttttt catgaattat aacgtcacct ttgcgaaatt ttccctcggt
     2941 taacggtgtt ttttcccacc ccagtattgt atgataacgg gaaaatttac gaaggtaagt
     3001 ctgcggcttc gccccggcaa ggcgcactag caattcgaga gtgactaccg aaagtactat
     3061 tccaaaagta ac
//
LOCUS       DAOQGF010000220         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_18961_length_3067_cov_5.927955, whole genome shotgun sequence.
ACCESSION   DAOQGF010000220 DAOQGF010000000
VERSION     DAOQGF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18961_length_3067_cov_5.927955"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2308
                     /locus_tag="PK937_16010"
     CDS             <1..2308
                     /locus_tag="PK937_16010"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH34128.1"
                     /translation="LYRREITERSIVFDQIVSLNNKSLELFVSDYSNWDDMVTFIHKP
                     DSVWAESNIDAVLSKFNVQLFLLCDRSFNVVYTTHINYTENNELIRTVTRDFQKSANS
                     GLFKHFYVLIGDTILEIRTAPVQPSMDVHRTTEPAGYLFVGRVWDDRYVNELADATQS
                     DMYISTSEASLLNDRVLPRDTIVVTRTFKNLSEKTIGWVSSIQKAEEFTELDRQANLQ
                     FILLVVFSTVLFLPLSLLLTVWVGRPLKIISDGLTDENAEKLQDLKNESSEFGTITRL
                     ILRANEQKILLVKEVQTRTEAEQALRRSERDYRELFENAHDAILIIDPVQFDVLQANR
                     EAARLYGYAQDEFMSLPLIKLWPSCSPELVDDIMQGRFPSYYETKHLRRDQSELVVEI
                     NAGIAQYRNQKVVVAISRDVTVKKLFEEVSMSHQILNRIGNMIIVFNNKGECIYANAA
                     TKHVLEYEPVELLGDGWWQITRKDAEERTQAKALKARKASGDILVNETPYLETYYKKN
                     GSMVWIEWHDTWDPGQMFIRIAYDVTDRVRADHAQNQIEMQRVRMATVVETQEEERKR
                     ISRELHDSVGQQLSAIKRNIEIIKQRFTASTENALSCDHLLSMVDTSLREIKLISYNL
                     LPRVLDELGLISALDTLCRTQFGAQGIHVDFQTFDVDQRLPQDIELPIYRVVQEAFQN
                     ITKHAKATQVTLQLIGYDDHVVVTIEDNGIGFDLASINEIKKAPFGMGLPGMRERIEF
                     LSGKIDIDSSPGNGTSVVIEVPFKKNEV"
     gene            2318..2989
                     /locus_tag="PK937_16015"
     CDS             2318..2989
                     /locus_tag="PK937_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNH34129.1"
                     /translation="MEKTDVLIADDHPMVREGLRSLLETEKGFNVVAEAGDGEEALKY
                     MERHKPMMAILDISMPKLNGIDTARQILKRWPKTRVVMLTMHHDEAFLVKVMEIGVQG
                     YLLKDSAGDEILRALKRIAEGEKYFSQSAMEAIGRHISKKNDPNVLADKEAAERLTKR
                     ELQILSYIAEGMSTPEIAKKLYLSPRTVDTHRANIMHKLNIHQATGLVRFALENGLFA
                     RDKTK"
BASE COUNT          931 a          587 c          733 g          816 t
ORIGIN      
        1 actctaccgt cgtgaaataa cggagcgaag cattgttttt gatcaaatcg tttcgctcaa
       61 taataaatct ctggaacttt tcgtgtcgga ttattccaat tgggatgata tggtcacgtt
      121 tattcataaa ccggattccg tgtgggccga aagtaatatt gatgcggttt taagtaaatt
      181 taatgttcaa ctcttcttgt tgtgtgatcg ttcgtttaac gttgtttaca cgacacacat
      241 aaattatacc gaaaataatg agctgatacg tacggtgacc cgtgattttc aaaaatcagc
      301 caactccggt ttgttcaaac atttttatgt tttgataggt gatacgatat tggaaatccg
      361 cacagcgccg gtacaaccaa gcatggatgt acatcgcacc acagagcctg cggggtattt
      421 atttgtagga cgggtatggg atgatcgcta tgtgaatgaa ttggccgatg cgacacaaag
      481 cgatatgtat ataagcacat ccgaagcatc tcttttaaat gatcgcgtgc tcccgagaga
      541 tacgattgtc gtaacgcgca catttaagaa tttatcggaa aaaactatcg gatgggtgag
      601 ttccatacaa aaagcagagg aatttaccga gttggaccgc caggccaatt tgcagtttat
      661 actgcttgtc gtattcagta cagttttatt tctgccgctt tctttgttgt taaccgtatg
      721 ggtcggccgt cctttaaaaa taatttctga cggtctgacg gatgaaaatg cagaaaaact
      781 acaagatttg aaaaacgaat catcagagtt cggcacgatc acgcggctta ttttgcgggc
      841 taacgaacaa aaaatattgt tagtaaaaga agtgcagact cgtacggagg cggagcaagc
      901 gctgcgaaga tcggaacgtg attatcgtga gctctttgaa aatgcgcatg atgccattct
      961 catcattgat ccggttcagt ttgatgtatt acaggccaat cgtgaagcgg cgcggttata
     1021 tggttacgcg caagacgaat ttatgagttt gccattgata aaactatggc cgtcctgttc
     1081 tccggagctt gtggacgata tcatgcaagg gcggtttccg tcgtattatg agacaaaaca
     1141 tcttcgacgt gatcaatccg aactggttgt agagatcaat gcgggcattg ctcaataccg
     1201 taatcaaaaa gtggtcgtag cgatcagtcg cgacgtaacc gttaaaaaac tttttgaaga
     1261 agtgagcatg tcgcaccaga tcctcaaccg catcggtaat atgatcatcg tgttcaataa
     1321 taaaggggag tgtatttatg cgaatgcagc gaccaagcat gtattagaat atgaaccggt
     1381 agaattactt ggtgacggat ggtggcaaat aacccgaaaa gatgccgaag aaagaacaca
     1441 agctaaagcg ctcaaagcgc gcaaggcgag tggggatatt ctggtcaatg aaacgccgta
     1501 tttggaaacg tattacaaaa aaaacggctc aatggtgtgg atcgaatggc acgatacctg
     1561 ggatccgggt caaatgttta ttcgtattgc ctatgatgtt acagacaggg ttcgagctga
     1621 ccatgcacaa aatcagatcg aaatgcaacg ggtacgcatg gccaccgtgg tcgagacaca
     1681 ggaagaagaa cgcaaacgca tttcacgaga actgcatgac agcgtgggtc agcaactttc
     1741 agcgatcaaa cgtaatatag aaataattaa gcaacggttt acggcatcca cggagaatgc
     1801 cttgtcctgc gatcacttgc tgtcgatggt ggatacaagc ttacgggaaa tcaaactgat
     1861 ttcctataat ctgttaccgc gcgtactgga cgagttgggc ttgatttctg cattagatac
     1921 attgtgtcgt actcagtttg gcgcacaggg tattcatgtc gattttcaaa cattcgatgt
     1981 agatcagcgc ctgccgcagg acatagaact gccgatatac cgtgtcgtac aagaggcttt
     2041 tcaaaatatt accaaacatg ctaaagccac acaggtcacc ctccaattga tcggatacga
     2101 tgatcatgtc gttgttacga tcgaggataa cggaattggg ttcgaccttg cttcaattaa
     2161 tgaaataaaa aaagcgccgt ttggcatggg tttaccggga atgcgcgagc gtattgaatt
     2221 tttgagcgga aaaatagata ttgattcgtc accgggtaac ggcacatccg ttgttatcga
     2281 ggtgccattt aaaaaaaatg aagtgtagta gtgtcatatg gaaaaaacgg acgtattgat
     2341 agctgatgat catccgatgg tacgtgaagg gttgcgttca ttgcttgaaa ctgaaaaagg
     2401 ttttaatgtc gtggccgaag caggagacgg ggaagaggcg ctgaagtata tggaacgcca
     2461 taaaccgatg atggccattt tggatatttc aatgcccaaa ttaaatggta ttgatacagc
     2521 tcgtcagata ttaaaacgat ggccgaaaac ccgcgtagta atgctgacca tgcatcatga
     2581 cgaagcgttt cttgtaaagg tcatggaaat aggtgttcaa gggtatttgc tcaaagattc
     2641 tgccggcgat gaaatactgc gtgcactcaa gcggatcgcg gaaggagaaa agtattttag
     2701 tcaaagcgct atggaagcga ttggccgcca tatttccaaa aaaaatgacc ctaatgtttt
     2761 agctgataaa gaagccgcag agcgtttaac aaagcgtgag ttgcaaattt tgagttatat
     2821 tgcggagggt atgagtacgc cggagattgc taaaaaatta tacctcagcc cgcgaactgt
     2881 ggatacacat cgtgccaata taatgcataa attaaatatt caccaagcta caggattagt
     2941 tcggtttgca ttagaaaacg gactttttgc cagagataaa actaagtaaa aacacctaag
     3001 ttaaaactct acataaatta cttccctcta cgtattgtta aagccaccca ttctatagta
     3061 tatagcg
//
LOCUS       DAOQGF010000221         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_19610_length_3007_cov_7.851626, whole genome shotgun sequence.
ACCESSION   DAOQGF010000221 DAOQGF010000000
VERSION     DAOQGF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19610_length_3007_cov_7.851626"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PK937_16020"
     CDS             complement(<1..997)
                     /locus_tag="PK937_16020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HNH34130.1"
                     /translation="MNSSSPKSLQLQVLQPALAKSKTSESITHELSLSGMHSSHCAGI
                     IENELRKLNGVQKADVDFANRRLIIRFYNDIVSIHTIIRKITDLGYGVNYTKKTLGVQ
                     GMTCAACAASVESMLLSQEGIFNAQVNFAAKTVFVEYASDRINVAEMKKVLQQIGYDI
                     SGDMENDADQIEVAEKKRYNELRLRMLVAILLSLPVFIIGMFFHNTWTWGNLISLILS
                     APVLFWAGRDFYINAFKQARHGTANMDTLVALSTGVAFIFSLFNTLFPHFFHQQGLHA
                     DVYYEAAVVIIALILLGRWLEEGAKNKTNSAIKKLMGLQPKTVRVLRDGNTLEIPV"
     gene            complement(1153..1704)
                     /locus_tag="PK937_16025"
     CDS             complement(1153..1704)
                     /locus_tag="PK937_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNH34131.1"
                     /translation="MTPFLQPTLQNERIYARPLQYEDFDALYAVASDPFIWEQHPNKD
                     RYKRDVFENYFRGAMESGGAFLICDAATRQVIGSSRYCNWNAETRTIEIGYTFIARDH
                     WGTTYNRALKTLMLDHAFTFAEAIQFFIGAVNVRSQNAITKLGAQKIAERETAYYGES
                     KKLDFVYEITKSTWFAIRDSKQL"
     gene            complement(1727..2236)
                     /locus_tag="PK937_16030"
     CDS             complement(1727..2236)
                     /locus_tag="PK937_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HNH34132.1"
                     /translation="MIVRLAEGTIKTLYGAYREYLYYDGQKESIALVMGDVRGENILC
                     RVHSSCLYGHAFNSMECDCREQMQISQKLIEKAGKGIVIWLEQEGKGNGHYALMQSKK
                     YKQQGMPQGEAYEAAGFNRDARDFRCAAEIIRDLGPSSICLITDNADKVQTLTQQGII
                     VSSTHPVAF"
     gene            complement(2265..2666)
                     /locus_tag="PK937_16035"
     CDS             complement(2265..2666)
                     /locus_tag="PK937_16035"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNH34133.1"
                     /translation="MEAYIFQFASLTLLGIAYYLVRRKQNFIAECDQVEGTVVRFRES
                     RRPGKNTTYAPVVEYFAYGKSYQHESSLYRSSPGFEIHQTVSVLYKRDKPEEAIIDSF
                     LEKWFIPMILGIVGSVFLIIGAVFPLLEREI"
     gene            2767..>3007
                     /locus_tag="PK937_16040"
     CDS             2767..>3007
                     /locus_tag="PK937_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNH34134.1"
                     /translation="MSKIRVESFTISLDGFGAGIEQTMNNPMGVGGQNLHQWAIPTKT
                     FQKALFGSDNGTTGIDDKFATRGFDNIGAWILGRNM"
BASE COUNT          895 a          624 c          605 g          883 t
ORIGIN      
        1 gaacgggaat ttctaatgta tttccatccc gcagaacacg aactgttttg ggctgtaaac
       61 ccataagttt tttgatagcg ctattggttt tgtttttagc accttcttcg agccatcggc
      121 caagtaatat caaggcgatg atgacaacgg cggcttcata atatacatcg gcgtgcaaac
      181 cttgctgatg aaaaaaatga ggaaagaggg tattaaaaag agaaaaaata aaagctacgc
      241 cggtactgag agctaccaaa gtatccatat tggcggtacc atgacgcgct tgtttgaacg
      301 cattgatata aaaatcacga ccggcccaaa acaaaaccgg agctgataag atcaaactga
      361 tcaagtttcc ccaagtccag gtattgtgaa aaaacatccc tatgataaaa acaggtaatg
      421 acagcagaat ggctacaagc attcggagac gaagttcatt atagcgcttt ttttcagcta
      481 cttcgatttg atcggcatcg ttttccatat ccccgctgat atcgtatccg atctgttgca
      541 gcactttttt catttcagcg acatttatac gatcggaagc gtattcgaca aataccgttt
      601 tggcagcaaa atttacctgc gcattaaaga taccttcttg ggaaagcaac atagattcga
      661 cactagccgc acatgctgcg cacgtcatgc cctgcacgcc taaagttttt ttagtgtagt
      721 taacaccata tccgagatct gttattttcc ggataatcgt atggatcgaa acaatgtcgt
      781 tatagaaacg aattatgaga cggcgatttg caaaatcgac atccgctttc tgcacaccat
      841 tgagcttacg cagttcattt tcgataatac cggcacaatg cgagctatgc attcccgata
      901 acgataattc atgcgtgatg gattcagatg ttttggattt cgcaagtgca ggttgtaaaa
      961 cctgaagttg cagagatttt ggagaggagc tattcatttt taaatacagg ttgaataaaa
     1021 taatctacca taatatacgg cggtgataaa aaaaaccgtt atatctgaat ggaaatttgt
     1081 tatatctaat tggctacaaa aaacagataa aactcaaacg aggagaattc gaagagctac
     1141 tacaatatta aatcaaagtt gtttggagtc gcgtatggca aaccacgttg actttgttat
     1201 ttcgtatacg aagtccaatt ttttggattc gccataatac gctgtttccc gctctgcaat
     1261 tttttgtgct cctaatttag taattgcatt ttgcgatcgc acattgactg caccgataaa
     1321 aaactgaatc gcttcagcaa aagtaaatgc atgatccagc atcagtgttt tgagcgcacg
     1381 gttataggtg gtaccccaat gatcgcgtgc aataaacgta taaccgattt cgatggttcg
     1441 tgtttctgca ttccaattgc aatatcgtga actgccaata acttgacgtg ttgccgcgtc
     1501 acaaatcaga aatgcgcccc cggattccat agccccgcga aaataatttt caaaaacatc
     1561 tcttttatac cggtctttat tagggtgttg ttcccaaata aacggatcac tggcgacggc
     1621 ataaagcgca tcaaagtctt cgtactgcaa cggccgcgcg tagattcgtt cattttggag
     1681 cgtgggttgt aagaaaggcg tcataattac ttttattgat gatttattaa aaagcaactg
     1741 gatgagtgct gctgacgata atcccttgct gcgttaatgt ttggacttta tccgcattat
     1801 cggtaatcaa gcaaatcgaa gatggaccca gatcgcgtat tatttcagcc gcacaccgaa
     1861 agtctcgtgc atctctatta aatccggccg cttcgtaggc ctcgccttgc ggcatgcctt
     1921 gttgtttgta ttttttgctt tgcatcaaag cgtagtgccc gtttcctttt ccttcttgct
     1981 caagccagat cactatccct ttgccggctt tttcgatcag tttttgtgaa atttgcattt
     2041 gttcacggca atcacattcc atactgttga aagcatggcc atataaacaa gacgagtgta
     2101 cacgacacag aatattttct ccgcgcacat ctcccatgac caaagcaata ctttcttttt
     2161 gcccgtcata ataaagatat tcacggtatg caccgtacaa agttttaata gttccttctg
     2221 caagtcgaac aatcatggtt actctttcaa ttcattacta aaattcaaat ttcacgttcg
     2281 agcaatggga aaactgcacc gataatcaaa aaaacagaac cgacaatacc caatatcatc
     2341 ggtataaacc acttttccaa aaaactatca attatcgctt cttcgggttt atcacgtttg
     2401 tataacacag atactgtctg atgtatttca aaacccggac tggatcggta aagtgaactt
     2461 tcatgttgat aagatttgcc ataggcgaag tattcgacca ccggtgcata cgtggtgttt
     2521 ttgcccggtc tgcgtgattc acgaaatctt accactgttc cttcgacttg gtcgcattcg
     2581 gcgataaaat tttgttttcg gcgtactaga taatatgcga tgccaagtaa agtcaggctg
     2641 gcaaattgaa aaatgtatgc ttccatctgt tgatttggcc ttagcggttt tggattttgt
     2701 ttcaatcaag acttgacaat aaaaacaaat ttacctcaat taattaaaat tcaaaggaag
     2761 aaaaatatgt ctaaaatacg tgtagaaagt ttcactatat cattggacgg attcggtgcg
     2821 ggcatagagc agacgatgaa taatcctatg ggggtgggcg gacaaaatct gcatcaatgg
     2881 gcgataccga caaaaacatt ccagaaagca ctgtttggtt ccgataacgg caccactgga
     2941 attgatgaca aatttgcaac acgaggtttt gataatatcg gtgcgtggat actgggtcga
     3001 aatatgt
//
LOCUS       DAOQGF010000222         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_19904_length_2978_cov_5.604516, whole genome shotgun sequence.
ACCESSION   DAOQGF010000222 DAOQGF010000000
VERSION     DAOQGF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19904_length_2978_cov_5.604516"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK937_16045"
     CDS             <1..671
                     /locus_tag="PK937_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNH34135.1"
                     /translation="WETRLATDEKIRFIAIEGVLKPENTALARNLSQMANARPILEAE
                     FLSPLVNIHSTDPKDMAFKKHMLHLMDRYKMLKQIHQTEIFFEQVICDYLFYADRVYA
                     NLKLDNEDMHLYDTMMNVMERELPIPDLVIYLQSNIDTVMEKIHTRHALGNASKERQR
                     MDETYIAALNEEFNQFFLYYRWSPVLIVNANQLDPENTHHLEDLYQRLDTRLSGVVYY
                     NPPA"
     gene            691..1746
                     /gene="amrS"
                     /locus_tag="PK937_16050"
     CDS             691..1746
                     /gene="amrS"
                     /locus_tag="PK937_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNH34136.1"
                     /translation="MRVPSSLRDNLRSLVQEGSLYKQTDDGRVQCYACGHRCIIGEGR
                     DGICKVRFNRNNKLWVPHGYTAGIQLDPVEKKPFFHVFPGSTAMSFGMLGCDLHCAYC
                     QNWITSQALQDPYAGVPPRLITAAELAQHASDHGARLLTSTYNEPLITAEWAVRVFEE
                     GQRLGLYGAFVSNGNATPEVLTFLRPYVSMYKVDLKSFNDKNYRKLGTTLKNVTDSIE
                     KIHAMGFWSEIVTLIVPGFNDSDVELQAMASFIKNISCDIPWHVTAFHPDYKMTDPDE
                     TDADTLARAAHIGKNEGLHFVYTGNRPGELRDMENTYCPGCKALLVKRHGYRIQQNRI
                     IHGKCPDCDRKIPGYWF"
     gene            1994..>2978
                     /gene="mltG"
                     /locus_tag="PK937_16055"
     CDS             1994..>2978
                     /gene="mltG"
                     /locus_tag="PK937_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNH34137.1"
                     /translation="MVIDDPRSEARKRLLQYIFIGISILVVATLLVVVSFYKLVLYNA
                     PSINVEQLPLDKKLLITIPKGASLNGISNVLQESGVIENATLFRLAARYLGAERSLKA
                     GQYLLPAHGSNHQIIRILQNAVPQSVRVTIPEGRGLRSIIQLLSAKLPLDSAKFVNAV
                     NDSLLCRELGFPDKSLFGYLMPNTYFIDPGTSESDIVRLMIAEYTNFFGDDLKKRTAE
                     LQLTPRQIMTIASIIEGETADDDERYLVSSVYHNRVRRGMMLQADPTIQFIIKDGPRR
                     LYNKDLEIDSPYNTYKVPALPLGPIGNPGLESLSAASNPINTYYLFFVSRNN"
BASE COUNT          828 a          710 c          617 g          823 t
ORIGIN      
        1 gatgggagac gcggttggcc actgacgaaa aaatacgatt tatcgctata gaaggcgtcc
       61 ttaagccgga aaacaccgcg ctcgcacgca atctttccca gatggccaat gcccgaccca
      121 ttttggaagc cgaatttctt tctccgttgg ttaatattca cagcaccgat cccaaagata
      181 tggcattcaa aaaacatatg ctgcacctca tggatcggta taaaatgctc aaacagattc
      241 atcagactga aatttttttt gaacaggtca tttgcgatta tcttttttac gccgatcgcg
      301 tatatgcgaa cctgaaactt gataacgaag atatgcatct gtacgatacg atgatgaatg
      361 tgatggaacg cgaattaccc ataccggatc tggtcatcta tcttcagagt aatatcgaca
      421 ccgtgatgga aaaaattcac acccggcacg cgctcggtaa tgcaagcaaa gaacggcagc
      481 gcatggacga aacctacatc gcagcgctca acgaagagtt caatcaattt tttctttatt
      541 accgttggtc accggtgctt attgttaatg ccaatcaatt agacccggaa aacacccatc
      601 acctcgaaga tctttatcaa cgtttagata cgcgcttatc cggcgtagtt tactataatc
      661 cgccggcctg aatccccgaa aggacggatc atgcgtgtcc catcatcatt gcgcgataac
      721 ctccgatcac ttgtccaaga aggatcactg tataaacaaa ccgacgacgg tcgtgtgcaa
      781 tgttacgcgt gcggtcatcg ctgcatcatc ggcgaagggc gcgatggtat ttgcaaagtt
      841 cgcttcaacc gcaataataa attatgggtt ccccacggat atacggccgg tatacaactt
      901 gatcccgtcg aaaaaaaacc attctttcac gtgtttcccg gcagcacagc gatgagtttt
      961 ggaatgctag gatgcgatct gcactgcgcg tattgccaaa actggatcac atcccaagcc
     1021 ttgcaagatc cctacgccgg tgttccgccc cgtcttatca ccgccgccga attggctcaa
     1081 catgcatcgg atcatggcgc acgccttctc acaagtacct ataatgaacc gctcatcacc
     1141 gccgaatggg cggtgcgcgt ctttgaagaa ggacagcgtc tgggtttata cggcgccttt
     1201 gtatccaatg gcaatgcgac accggaagtt ttaacttttt tgcgcccgta tgtttctatg
     1261 tacaaagtcg atctcaaaag ttttaacgac aaaaattatc ggaagctcgg aacgacatta
     1321 aaaaatgtaa ccgatagcat agaaaaaatt catgccatgg gtttttggtc ggaaatcgta
     1381 accctgatcg ttccgggctt taatgattcg gatgtcgaac tacaggccat ggcatcattt
     1441 ataaaaaaca tttcatgcga tataccctgg catgtcacgg cgtttcatcc tgactacaag
     1501 atgaccgatc cggacgaaac cgatgccgat acattagcac gcgcagcaca tatcggtaaa
     1561 aacgaaggtt tgcattttgt gtataccgga aaccgtccgg gtgaactgcg tgatatggaa
     1621 aacacgtact gcccggggtg caaagcattg ttagtaaaac gccacggtta tcgcattcag
     1681 caaaatagga ttatccacgg aaaatgcccg gattgcgatc gcaaaattcc cggctattgg
     1741 ttctaaattg atcccttata gtaatcatta gttttcaatt gaaataaata acactggtat
     1801 aagtgtgtat gtatttaatc ttcaattgat tattttttaa catcttttct taataacgta
     1861 atggctaata cttcttattc aacacgctga tccaatctcc tacagaaatt tagtttaatc
     1921 caaaatattt gattccaagc tttctttttt atatacttcg cgcggtttcg gtttcactaa
     1981 gaaggaaaat tcattggtta ttgatgatcc gcgctccgaa gcacgtaagc gcttattgca
     2041 atacattttt atcggcatca gtattctcgt cgttgctaca ttgcttgtcg ttgttagttt
     2101 ttacaaactt gttttgtata acgcgccttc gatcaatgtc gaacaattac cgttagataa
     2161 aaagttactc attactattc ccaaaggcgc atcgttgaat ggcatttcga acgtcttaca
     2221 ggaaagcggt gttatcgaaa acgctacgct ctttcgtttg gccgctcgct atctcggcgc
     2281 agaacgaagt ctgaaagcag ggcaatatct tttgccggca cacggttcca atcatcagat
     2341 catccgcata ttacaaaatg ccgtacccca aagtgttcgt gtgacgattc cggaaggccg
     2401 cggcttacgc tccatcatac agcttttatc ggccaaactt ccgctcgact ctgccaaatt
     2461 tgtcaatgcc gtcaatgact ctttactctg tcgtgaatta ggattcccgg ataaatcgct
     2521 ctttggttat ctgatgccca acacttattt cattgatccc ggaaccagcg aaagtgacat
     2581 cgttcgattg atgatcgcgg agtatactaa ttttttcggc gatgacttaa aaaagcggac
     2641 cgctgaattg caattgactc cgcgtcagat catgaccatc gcctccatca ttgagggcga
     2701 aactgcggac gatgacgagc gatatttagt ttcatccgtt tatcataatc gtgtacgccg
     2761 tgggatgatg ctgcaagccg atccgaccat acagttcatc atcaaggatg gtccgcgccg
     2821 cctgtacaac aaagatttgg aaatagacag tccttacaac acatataaag ttcctgcatt
     2881 gccactaggt ccgattggga atcctggttt agaatctcta tccgcagcat ccaatccaat
     2941 aaatacttat tatttatttt ttgtcagccg aaataatg
//
LOCUS       DAOQGF010000223         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_20834_length_2902_cov_6.662803, whole genome shotgun sequence.
ACCESSION   DAOQGF010000223 DAOQGF010000000
VERSION     DAOQGF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20834_length_2902_cov_6.662803"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK937_16060"
     CDS             <1..728
                     /locus_tag="PK937_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNH34138.1"
                     /translation="SLYEPMALVVGGMICIAAANAGATSQDLKTGYIVGATPKYQQIA
                     LFVGAIASSAMIGWTVQILDTPTSEMAAQGIQHAIGTEKYPAPQGTLMATLIKGLLSF
                     NLDWQFVLVGAFVAVTMELCGVKSLSFAVGAYLPLSTTLPIFAGGAIKGIADWNRSRK
                     GESEEDAELGKGSLFATGLVAGGALFGVIVAIATVFAEDFMKSINMEHTFSGILGQAG
                     YEIFGVICFALMGITLYRVAMKK"
     gene            950..1699
                     /locus_tag="PK937_16065"
     CDS             950..1699
                     /locus_tag="PK937_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine-4-hydroxylase"
                     /protein_id="HNH34139.1"
                     /translation="MTLENENHTTEELEVWRLLFERQSSLLHNAACQTFLEGLTVLDF
                     QPDAIPHLKSINAKLRDMTGWSLTVVPGIVEDAIFFEMLSRKTFPVTGWLRSKKQLQY
                     IEEPDMFHDVFGHVPMLTDKQFCGFLEGLSRIALAYPNNTFIIERLVRMYWFTVEFGL
                     VRESDNVKICGAGILSSIGETLYCLSGKPPVVEFDLHTVFEMPFRKDRFQDRYVMISD
                     FEQLFRSVYQMEETLLRLQRQIQLQVLSWSC"
     gene            1771..>2902
                     /locus_tag="PK937_16070"
     CDS             1771..>2902
                     /locus_tag="PK937_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34140.1"
                     /translation="MNSANKSNEIERIADMLRLEMNNRPSEDFIKSFIRQIDSLAPNV
                     LRYFRSTLSQTLIERENQGKGHLKIEALLELLDLCDLQVWLTSAGLHEKAARNVVKGL
                     KNRSTEELNRIIHGKLYIEHDRTELLEFLVDAGVEGDDAEHFVKLVREMDVSVTEIID
                     RIFKLNFVAENAFRQESLRGMMSLKEIVTLYLYSVLQVTISDYVHDEETISEIAARLA
                     LCNNMRREFALVQQLERYFFSTTSVEDTQNKTRDFWIKLLNDYEVKLALEQYIGDDKL
                     KRSDMEIADLYVEAGHIFDDVSEIKDQTLAVISITYRLDNPEETGHTVEELLAIIDQI
                     KLLTTEMGPLSIMTRDIFSFFEILGESDHKNHFFHKTIISFRS"
BASE COUNT          796 a          630 c          652 g          824 t
ORIGIN      
        1 gcagcttata tgaaccgatg gcgcttgtcg tcggcggtat gatctgtatc gcagcggcta
       61 acgccggtgc gacatctcag gatcttaaaa ccggctacat cgttggcgcg acaccgaagt
      121 atcagcagat tgcgcttttt gtcggtgcga ttgccagctc cgcgatgatc ggatggacgg
      181 tgcaaatcct tgatacgccg acttctgaaa tggccgcaca aggtattcag catgcgatcg
      241 gcacggaaaa atacccggca ccccagggaa cattgatggc taccttgatc aagggtttgt
      301 tgtcttttaa ccttgattgg cagtttgttc ttgtcggcgc atttgtagct gtgacaatgg
      361 aactctgcgg cgtcaaatcg ttatcgtttg ctgtaggcgc atacttacct ctttcgacga
      421 cacttcctat ttttgccggc ggcgccatca aaggtattgc cgattggaat cgctcacgta
      481 agggtgaatc ggaagaagat gcggaacttg gcaaaggcag tctttttgcg acaggtcttg
      541 ttgccggcgg cgcgctcttt ggcgtcatcg ttgccatcgc aactgtgttt gccgaagact
      601 tcatgaaatc catcaatatg gaacatactt tctccggtat tttgggccag gccggttatg
      661 aaattttcgg tgttatctgt tttgctctca tgggcattac actgtaccgg gttgcaatga
      721 aaaagtaagt gccgtgaact gtttttgact ttagagagaa cattacttat tatttgatat
      781 atgagaacag cgcctataca tatagaaaca tcgttttatt attactttta tcgcaacgga
      841 tccccgcttt ggagatgcga ttaaggtagt ttttatttta aattcaccaa cccgtttccc
      901 caaaggaaac gggtttttta ttttcaccct ttaatgaaag gaaaacgcca tgactttgga
      961 aaatgaaaac cacacaacag aagaactcga agtttggaga cttttgttcg aacgccaatc
     1021 gtcactgctg cacaacgcag cgtgccaaac gtttttggaa ggactcaccg tattggattt
     1081 tcaaccggat gccattcctc atttgaaatc aatcaatgca aagcttcggg atatgaccgg
     1141 ttggtcgctc acggtagtac ccggcatcgt tgaggatgcg attttttttg aaatgctgtc
     1201 gcgtaaaaca tttcccgtta caggatggtt gcgatccaaa aaacaacttc agtatatcga
     1261 ggagccggat atgtttcatg atgtattcgg acacgtcccc atgttaaccg ataaacaatt
     1321 ttgcggtttc ttggaaggat tgagccgcat cgccttagcc tatcccaaca acacctttat
     1381 tattgaacgc ctcgtacgta tgtattggtt tacggtcgaa ttcggtttgg tacgtgaatc
     1441 cgataacgtc aagatttgcg gcgccggaat cctttcatcc atcggcgaaa cgctgtattg
     1501 cctcagcggc aaaccacccg ttgtggagtt cgatttacac accgtatttg aaatgccctt
     1561 ccgaaaggat cgttttcaag atcggtacgt gatgatcagt gattttgagc aattatttcg
     1621 atccgtgtat caaatggaag aaactttgtt acgtttgcaa agacaaattc aacttcaggt
     1681 tttatcatgg agctgttgag cgataaaact gaaaacaatt gaaaaacacg tttttatggc
     1741 taaatttagc acattcaaaa aaggttacgg atgaattcag ccaataaatc aaacgaaatc
     1801 gaacgcattg cggacatgct tcgactggag atgaacaatc gtcccagtga agattttatt
     1861 aaatcgttta ttcgccaaat agacagcctt gcgcccaatg tattacggta ttttcgctct
     1921 acgctcagtc agactttgat cgaacgcgaa aatcaaggca aaggacatct taaaatcgaa
     1981 gcgcttcttg agttattgga tttatgcgat ttacaggtgt ggcttacttc ggcaggactg
     2041 catgaaaaag ccgcccgtaa tgtcgtcaaa ggattaaaaa accgatccac tgaagaactc
     2101 aatagaatca ttcacggcaa gctctacatc gaacatgatc gcacggaatt acttgagttc
     2161 ttggttgatg ccggtgttga aggcgatgat gccgaacatt ttgtgaaact tgttcgtgag
     2221 atggatgtgt ccgtaacgga aattattgac cgcatcttca aacttaattt tgttgccgaa
     2281 aatgcgttcc gacaagaatc cctgcgtggt atgatgtcgc tgaaagaaat tgtgacgtta
     2341 tacttatact cggttttaca agttacgatc agtgactatg tgcatgacga agaaacaata
     2401 agcgaaatag ccgcgcgcct cgctttatgt aataatatgc gccgtgaatt tgccttggtt
     2461 cagcaattag aacgctactt tttcagcacc acatccgtag aagacaccca aaacaaaaca
     2521 cgggattttt ggatcaagct gcttaacgat tacgaagtga agctcgccct ggagcaatat
     2581 atcggtgatg ataaattgaa acgctccgat atggagatcg ccgatctcta tgttgaagcc
     2641 gggcacattt ttgacgatgt cagcgagatc aaagatcaga ccttagccgt catttcgatc
     2701 acctaccggc ttgataatcc cgaagaaaca ggacacaccg tcgaagaact tcttgccatc
     2761 atagatcaga tcaaattgct cacaaccgag atgggcccgc tgagcattat gacaagagat
     2821 attttttctt tttttgaaat cctcggtgaa tccgatcaca agaatcattt tttccacaaa
     2881 acaattattt cattccgttc gt
//
LOCUS       DAOQGF010000224         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_21219_length_2871_cov_6.765270, whole genome shotgun sequence.
ACCESSION   DAOQGF010000224 DAOQGF010000000
VERSION     DAOQGF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21219_length_2871_cov_6.765270"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK937_16075"
     CDS             <1..196
                     /locus_tag="PK937_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNH34141.1"
                     /translation="DRDGVGDEPYRPVSLFSVISEQHTEAMILMRSLTIVALDLVERI
                     IPSLTPAALLDDKPKMGRCL"
     gene            193..927
                     /locus_tag="PK937_16080"
     CDS             193..927
                     /locus_tag="PK937_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNH34142.1"
                     /translation="MITIQSLTKNFGEQRVLNRVNASFDTGKVTAIIGPNGSGKSTLI
                     KSILGLVTPDSGLITLDGKAIAPQDAEYRRRIGYMPQTARFPENMKVHELLSLMADLR
                     DMVTDRDEELMRSLQLEREISKALKHLSGGTKQKVNAYLAFLFRPDVLILDEPTAGLD
                     PVSSTLLKDKILKEKNNGRTVIITSHVMSELEEITDRVLFLNEGCVNFEGTIEDLRQK
                     TGEERLERSIAHLMKGLGLWTLSKKY"
     gene            903..1694
                     /locus_tag="PK937_16085"
     CDS             903..1694
                     /locus_tag="PK937_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNH34143.1"
                     /translation="MDALQKILKFEWYDQIRNKWIVLYTAFFFVLTFGLFQLQGDASR
                     VFLTMTQILLLIVPLVSMIFGAMHVYHSREFIEILLCQPIRRSKLFWGVYLGMALPLA
                     GGFVVGAGVPILMFGYNSIPDWRIPVMLLGTGLLLTMIFSALAFGIAMRTDDKLKGIG
                     FALGLWFALALLFDGMVLYIVYAFADYPLEKPLIGISMLNPINIARSMILMKMDVAAM
                     MGYTGAAFKKFFGGSLGVIVSLAALVGWAVIPLFFAKRHFLKKDF"
     gene            1698..2207
                     /locus_tag="PK937_16090"
     CDS             1698..2207
                     /locus_tag="PK937_16090"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNH34144.1"
                     /translation="MLTGYFISVFLHILCAMIWVGGLIFFVIVFVPLLRNPEYKNVAP
                     KIVLWAGERFRFWGWIVFGLLLTTGLYNVFARGYSWGDFFKSEFYDSHFGHTLGIKIT
                     LFMLLLVLGGLHDFWLGPKATRMWRDTPDHPSVPGLRKAASWIGRVNLLISLMIVFLA
                     IMIVRGNPW"
     gene            2209..2580
                     /locus_tag="PK937_16095"
     CDS             2209..2580
                     /locus_tag="PK937_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34145.1"
                     /translation="MRIFNIKIPALLLVVTLFSAGLPQAFRAISENNIKTCCCLSQGK
                     ACTCDHKNVTQADAGACSMRNEGCGTKGNGEMQRESLIAVHIQPAIVIAEFHYTTILS
                     SFTQPIFISKTPSDIFHPPRA"
BASE COUNT          691 a          633 c          706 g          841 t
ORIGIN      
        1 ggatcgcgac ggagtcggtg atgagccgta tcgtcccgta agtctttttt ccgtgatttc
       61 cgaacaacat acggaagcga tgatcctcat gcgcagcctc acgatcgtcg cgctcgatct
      121 ggtcgagcgc attatcccgt cgcttacacc ggcggcgctt ttggacgaca aacccaaaat
      181 gggacggtgc ttatgataac aatccaatct ctaaccaaaa attttggcga acagcgcgtg
      241 ctcaatcgcg tcaacgcttc gtttgatacc ggaaaagtca cggccatcat aggacctaac
      301 ggttccggca aatccacgct tatcaaatcc atactcggtt tggtaacacc cgacagcggt
      361 ttgatcacgc tggacggcaa agcgatagca ccacaggatg ccgaataccg tcgtcgtatc
      421 ggatatatgc cacagaccgc acgatttccc gaaaacatga aagtgcacga actattatcg
      481 cttatggccg atcttcgcga tatggttacg gatcgcgatg aagagttgat gcgttcgctg
      541 cagctcgagc gggaaatcag taaagcgctc aaacacctct ccggcggaac caaacaaaaa
      601 gtgaatgcgt acctcgcttt tctttttcgg ccggatgtac tgatattgga tgaacccacc
      661 gccgggcttg atcccgtatc gagtacgtta ctcaaggata aaattctcaa agaaaaaaat
      721 aacggccgca cggtgatcat tacgtcgcac gtgatgagtg aattggaaga aatcaccgat
      781 cgtgttttgt ttttaaatga agggtgcgtc aattttgaag gtacgatcga agatctgcgt
      841 caaaagaccg gcgaagaacg gctcgaacga tccatcgcac atttgatgaa aggacttgga
      901 ctgtggacgc tctccaaaaa atactaaaat tcgaatggta tgatcagatc cgtaataaat
      961 ggatcgtatt gtacacggct tttttctttg tgctgacctt cgggttattt caactgcaag
     1021 gggatgcaag tcgcgtattt ctgacgatga cgcagatttt gcttctcatc gtgccgctgg
     1081 taagtatgat attcggcgcc atgcatgtat atcactcgcg cgaatttatt gaaattcttt
     1141 tgtgccaacc gatacgccgc agcaaactat tttggggcgt gtatctcgga atggcgctgc
     1201 cgctggcagg agggtttgtc gtcggtgcag gtgttccgat actgatgttc ggttataact
     1261 cgataccgga ttggcgaatc cctgtgatgc tgctggggac aggtttattg cttacgatga
     1321 tttttagcgc gctggctttt ggtattgcga tgcgcacaga tgacaaactc aaaggcatcg
     1381 gttttgctct tggcctgtgg ttcgctctag cgttgctttt tgacggtatg gtgttataca
     1441 tcgtgtatgc cttcgccgat tatccgttgg aaaaaccgct gatcggcata agtatgctca
     1501 atcccatcaa tattgcgcgc tcgatgatac tgatgaaaat ggatgttgcc gcgatgatgg
     1561 gttataccgg ggctgcgttt aaaaaattct ttggcggttc tctcggtgtt attgtatctt
     1621 tggcggcctt ggtagggtgg gcagtgatac cgcttttttt tgccaaacgg catttcttaa
     1681 agaaggattt ctaaatcatg ttgaccggtt attttatatc cgtatttctt catattcttt
     1741 gtgccatgat ttgggtaggc ggtttgattt tttttgtgat cgtgttcgtt ccacttctcc
     1801 gcaatccgga gtataaaaat gtggcaccga aaatcgtatt atgggccgga gaacgttttc
     1861 gtttttgggg ttggattgta tttggtttgc tgctcaccac cggtttatac aatgtatttg
     1921 ctcgagggta ttcctggggt gattttttta aatccgaatt ttatgacagc catttcgggc
     1981 acacactggg aatcaaaatc acactgttta tgctgctatt agtacttggt ggtttgcatg
     2041 atttttggct tggacccaaa gccacccgta tgtggcgcga taccccggat catccgtcgg
     2101 tacccggact gcgcaaagcg gcgtcttgga tcggacgtgt gaatctttta attagtctga
     2161 tgattgtttt tttggccatc atgatagttc gtggaaaccc gtggtgatat gcgtattttt
     2221 aacatcaaaa taccggcgct tcttttagtg gtaacactat tttcagcggg attgccacag
     2281 gcttttcgag ccattagcga aaacaacatc aaaacatgtt gctgtttgtc gcaaggtaaa
     2341 gcatgtacct gcgaccataa aaatgtcaca caagcggatg cgggcgcctg ctctatgcgc
     2401 aatgaaggct gcggcaccaa aggtaacggt gaaatgcaac gcgaatcatt gattgcggtt
     2461 catatccaac cggccattgt tattgccgaa tttcattata cgacaatact ctcctctttc
     2521 actcaaccga ttttcatttc aaaaacgcct tctgatattt ttcacccgcc gcgcgcctaa
     2581 actcttttcc aaacaacgga tgctcggtgc atcgttcttt ccgtacgatg tcaggcttcc
     2641 ggttaataca agttgatcat cgtgttggta cgccgacata ctctgagtat gagcgttagc
     2701 gacgtaccgg agtgtagtgt atagtcacga tgggaggtca cgttttatgc gtgctgccgt
     2761 tgaacttcaa aaaatgaagc ttggagcgac gcgtgtgcta agtatgtaaa aaatttttgg
     2821 gagcgtttat catgttacgt tggttagcaa ttatcataat gactgctttt t
//
LOCUS       DAOQGF010000225         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_21490_length_2847_cov_5.151504, whole genome shotgun sequence.
ACCESSION   DAOQGF010000225 DAOQGF010000000
VERSION     DAOQGF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21490_length_2847_cov_5.151504"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="ftsH"
                     /locus_tag="PK937_16100"
     CDS             <1..1252
                     /gene="ftsH"
                     /locus_tag="PK937_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNH34146.1"
                     /translation="LARAVAGEAEVPFFSISGADFVEMFVGVGAARVRDLFEQAKRSH
                     PCIIFIDEIDAVGRSRGAGLGGGHDEREQTLNQLLVEMDGFEENSGIIILAATNRPDI
                     LDNALMRPGRFDRQVVVDYPDVKGREEILKIHCRKVPISDEVKIEKIAKGTPGLAGAD
                     LENLVNEAALLAARRNSDKVENIDFENAKDKVMMGVERKSMVISDKEKRSTAYHEAGH
                     ALVGRLLPESDAVHKVTIIPRGRALGITHYLPSDDRHSYSRTYIEARLAMMMGGRAAE
                     KIIYNHYTTGASNDIERATEMARRMICEWGMSDALGPVAFGKKDEEVFLGREIATSKN
                     YSENTAVQIDAEIKRIVSEAENKALSLIEQNIQTLHNIANALVERETIDSEELDMILK
                     GQQLPPLEILNNGQSAAKIEAPVA"
     gene            1344..>2847
                     /locus_tag="PK937_16105"
     CDS             1344..>2847
                     /locus_tag="PK937_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34147.1"
                     /translation="MDKILYWLSGGVPGGFGIWFAVVAALCFIWWFLHRYSPVVNHSG
                     LARKMMIVLGLIVLTYTTIRFARPPKPFNVYVGILPLENQGSNKSLSVAGTGWGLAEI
                     TTHHAALGSPEHLHFLRPEWLVESYFGDSTSAISPSNKQKLFRWARLIKLNYLVAGFY
                     NADANPVSMSYEVYDVLTGETMRSFTNTIPTGDEVPYGQAMRTAAEELMTYLYEVTDE
                     EFNSAADHTDLFQTKSLPLYFSARDLLAHEQWDSAFAVARMALQKDSASVLGWFAAGS
                     AYTEKMIREKDARAREMLQRRGEYHLKRGAQINDQYQPILMSLARNFSYTKPEPRYLD
                     AEFAMIASQAIYKRDYQLYYILSFMQRMRWETFEMKSREEILNRAIEINPASFDAYVA
                     LGQDFLERSRPHDHLSQYALNNFNVALQLKPNDFESIMGMVRACDYLAYFDKAVELLE
                     RAEKFYPDRADVYYTYGVIYYHKGEYIREKTKLREGHEQFPIAEGYFKKAV"
BASE COUNT          753 a          680 c          730 g          684 t
ORIGIN      
        1 attggcaaga gccgtcgccg gtgaagccga agttccgttt ttttccatca gcggtgcgga
       61 ctttgtggaa atgttcgtcg gggtcggcgc ggcacgtgtg cgcgaccttt ttgaacaggc
      121 caaacgcagt cacccgtgca ttatatttat tgacgaaatt gacgccgtag gccgttcgcg
      181 cggcgccggt ttgggcggag gtcacgacga acgcgaacaa acgctcaatc aattgctggt
      241 cgaaatggac ggctttgaag aaaacagcgg tatcatcata ctcgcggcaa ccaaccgccc
      301 cgacattttg gataatgcgt tgatgcgccc cggccgcttt gaccgtcaag tcgtcgtgga
      361 ttatccggat gtcaaaggcc gtgaggaaat tttaaagata cattgccgga aagtaccgat
      421 cagtgacgaa gtaaaaatcg aaaaaatagc caaaggaacg cccggtttgg ccggagcgga
      481 tttggaaaac ctcgtcaacg aagcggcttt gctcgcagcg cgtcgcaatt cggataaagt
      541 cgaaaacata gattttgaaa acgccaaaga taaagtcatg atgggcgttg aacgaaaaag
      601 tatggtcatc agcgacaaag agaaacgttc gacggcttat catgaagccg gtcatgctct
      661 ggtaggtcgt cttttgccgg aatccgatgc ggttcacaaa gtcacgatca ttccccgcgg
      721 tagagcgctc ggcatcaccc actatctccc ctcggatgat cggcattcgt attcgcggac
      781 gtatatcgaa gcgcgacttg cgatgatgat gggtggccgt gcggcagaaa aaatcattta
      841 caaccactat acgacgggcg ccagcaacga catcgaacgc gctacggaaa tggcgcgtcg
      901 catgatctgc gagtggggca tgagcgacgc attaggtccg gtggcctttg gaaaaaaaga
      961 cgaagaagtt tttctcggac gcgaaatcgc aacatccaaa aactatagcg aaaatactgc
     1021 ggtacagatc gatgcggaaa tcaaacgtat cgtctcagaa gccgagaaca aagcattgtc
     1081 ccttatcgaa caaaatattc agacactgca caatattgcc aatgcgcttg tggagcgcga
     1141 gaccattgat tcggaagaac tggatatgat actcaaaggg caacagctgc caccgctcga
     1201 aatcctaaat aatggacaga gtgcggctaa aattgaagcg cccgtggcat aatcgccatg
     1261 ttgttttgat tgcagtttgg cggtaataat cgccaagctt tgtatgcgct tagggttcta
     1321 atagcgaacg ggtaggaaaa aatatggata agatattgta ctggctctcg ggcggcgtgc
     1381 ccggcggctt tgggatttgg tttgcggtcg ttgcggcact ttgttttata tggtggtttt
     1441 tgcatcgtta ttctccggtc gtcaatcatt ccggattagc tcggaaaatg atgatcgtgc
     1501 tcggcctcat cgtcctcacg tacaccacga tacgtttcgc gcgcccgccg aaaccattta
     1561 acgtatatgt gggaattctt cctctggaaa accaaggctc caacaagagt ttgtccgtgg
     1621 ccgggacggg ttggggctta gccgaaatca cgacgcatca cgccgcactc ggatcacccg
     1681 aacatttaca tttccttcgt ccggaatggc tcgtcgaatc gtatttcggg gactcgacct
     1741 cggccatttc accatccaac aaacaaaaac ttttcagatg ggcgcgcctg atcaagctga
     1801 attacctcgt tgccggtttt tataatgccg acgccaatcc ggttagcatg agttatgagg
     1861 tttatgatgt gttgaccggc gaaacaatgc gttcttttac aaacaccatc cccaccggtg
     1921 acgaagtgcc ttacggacaa gcgatgcgga cggccgcaga agaattgatg acctatttat
     1981 acgaagttac ggatgaggag tttaactccg ccgccgatca caccgacttg tttcagacca
     2041 aatcgctacc tttatatttt tcggcacgcg accttttggc gcatgagcaa tgggattctg
     2101 cttttgcggt ggcccgcatg gcgttacaaa aagacagcgc ttccgtttta ggatggtttg
     2161 ccgcgggttc ggcttataca gaaaagatga ttcgtgaaaa agacgcgcgc gcgcgcgaaa
     2221 tgcttcaacg tcgcggagaa taccatctca aacgcggtgc gcaaatcaat gatcaatacc
     2281 aacccatttt gatgagcctg gcacgcaatt tcagttacac caaaccggaa ccgcgttatt
     2341 tggacgccga atttgcgatg atcgcaagcc aagccatata caaacgggat tatcagctgt
     2401 attacatctt gagttttatg caacgcatgc ggtgggaaac atttgaaatg aaatcccgcg
     2461 aggaaattct caatcgcgcc atagaaatca atccagcgtc ttttgatgca tacgttgcct
     2521 tgggccaaga ttttttggag cgcagccgac cccatgatca tctttcgcaa tatgcattga
     2581 ataactttaa cgtcgcatta cagctcaaac ctaatgattt cgagtctatt atgggtatgg
     2641 tcagagcgtg cgattatttg gcttacttcg acaaagcggt cgagctgttg gaacgtgctg
     2701 aaaagttcta tccggatcgt gccgatgttt attataccta cggtgtgatt tactatcaca
     2761 agggcgagta cattcgcgaa aaaacaaaac tgcgcgaagg gcatgaacaa ttccccattg
     2821 ccgaagggta tttcaaaaaa gccgtgc
//
LOCUS       DAOQGF010000226         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_22206_length_2792_cov_6.141396, whole genome shotgun sequence.
ACCESSION   DAOQGF010000226 DAOQGF010000000
VERSION     DAOQGF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22206_length_2792_cov_6.141396"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK937_16110"
     CDS             complement(<1..866)
                     /locus_tag="PK937_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNH34148.1"
                     /translation="MLSSSKILIIDDDEDVLLAARLFLKQHVETVRTEKNPERIPHML
                     KEEAWDVIFLDMNFTRDVSSGIEGFQWLERILQIKPDAVVIMITAYGDVDMAVRAIKA
                     GAMDFVLKPWQNEKLLATLSAAVRLRVSRREADQLRDREKQLVQAMESPMAEFIGQSE
                     AMKKVFATIEKVAATDANVLILGENGTGKELVARALYHRSHRKDKPFITVDLGAISET
                     LFESELFGHVKGAFTDAKEDRVGRFEVAGGGTLFLDEIGNLSLALQSKLLTAIQKNEI
                     TRLGSNKPIAVNA"
     assembly_gap    1173..1272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1702..2097)
                     /locus_tag="PK937_16115"
     CDS             complement(1702..2097)
                     /locus_tag="PK937_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNH34149.1"
                     /translation="MKDILDLLREPQVTIAVVGANNNPNKYGHTIYRDLRRKGYTIFP
                     VNPSATEVEGDKAYANLAALPVKPTIINFVVPPSVTLNILQECLELGHTNVWLQPGSE
                     NDEVLNFLETRRFNYLANACIMIESRVLH"
     gene            complement(2101..2724)
                     /locus_tag="PK937_16120"
     CDS             complement(2101..2724)
                     /locus_tag="PK937_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34150.1"
                     /translation="MSEEKKEHFVGRLIFELLIVFAGVYGAFVVSDFQDQQRAKRARI
                     AFLQTFREDLVRMDRQMVIPRMQVLDSLIVFYESAMAAGKKPELKFHPTIDFTSTMLV
                     VRAAFNSDYLENIEREHIVSISQGSNLITLIEKKSDVFQNECRRWFHGPSYTNDDFYD
                     RSGRLKPGMQWYLDDMKELRHLLQILDIAIMQGAVKSTDHLLKKEME"
BASE COUNT          709 a          655 c          587 g          741 t
ORIGIN      
        1 gcattgaccg cgatcggttt attcgagccg aggcgggtga tttcattttt ttgaatggcc
       61 gtcaacaatt tggattgcaa ggccaacgaa aggtttccaa tctcatccaa aaacaatgta
      121 ccgccgccgg ccacttcaaa acgacccacc cgatcttctt tggcatcggt aaaagcgcct
      181 ttcacatgac cgaacagttc gctttcaaaa agcgtttcgc tgatggcgcc gagatcgacg
      241 gtgataaaag gtttgtcctt acgatgggaa cgatgataca aggcgcgtgc gaccaattct
      301 ttacccgtcc cgttttcacc gaggatcagc acattggcat cggtcgcggc gactttttcg
      361 atcgtggcga aaactttttt catcgcttca ctttgaccga taaactccgc catcggcgac
      421 tccatcgcct gcaccaattg tttttcacga tcacgcaatt gatcggcctc acgccgcgaa
      481 acgcgcagcc gtaccgccgc ggataacgtc gctaagagtt tttcgttttg ccagggtttg
      541 agaacaaaat ccatcgcccc cgctttgatc gcccgcacgg ccatatcgac atccccgtag
      601 gcggtgatca tgatcacgac ggcatccggc ttgatttgca aaatacgctc aagccactgg
      661 aaaccttcga ttccgctgct cacatcgcgg gtaaaattca tatccaaaaa aatcacatcc
      721 cacgcttctt ctttgagcat gtgcgggatg cgctccggat ttttttccgt acgaacggtt
      781 tccacgtgtt gcttcagaaa aagacgcgcg gccaaaagca cgtcttcgtc gtcatctatg
      841 atcagaattt tggaggaaga aagcatagct gtaattatat tttcaggatg attatcgatg
      901 catttctaac gattggtaat gtgttggtct aaagaaccgt ggccgcattc acaaaatatt
      961 tattaccaaa aaaccgtttc gttcgttttt acagaaacac gtttacatac gcatggcgcg
     1021 ctgttttgtt tgtttttaat ttccgtgaaa tgtgcgaaaa ttaatagtta atacgaacaa
     1081 ctacattgga acaaccgttt taatccatca aatccatact taaagatgca acgtcctgtt
     1141 tttagtgggt tcccgctttc gcgggaacga cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnacttaaag atgcaacgtc ctgtttttag tgggttcccg ctttcgcggg
     1321 aacgacacgc tttcgcggga acgacatgct ttcacgggcg tggcaacgtg aagttattcg
     1381 tattgtctca cccgctttcg tgggtatcta tgtgaccaca ggtttagcat tgtggttaac
     1441 gaatttattt cttctccgtg ggttcccact ttcgcgggaa tgacatgctt gcgccggaac
     1501 ggtaaccttt taaggaaacg gcaagacaat gttttgtgtt ttgccttacc cgcactgtca
     1561 tttgcgcaga agtggaagaa gtatccataa aaaattttgt aagcccagtt tgatctacaa
     1621 atttcagtaa tcaggatcga accatatatc tacagatcaa acaaacgtaa aatcagagat
     1681 gcttaatata tgaaaaaccg atcaatgcag aacgcggctc tcgatcataa tacacgcatt
     1741 ggctaaataa ttgaatcgcc gggtttcaag aaagttcagc acttcgtcgt tttccgaacc
     1801 gggctgcagc catacattgg tatgtccgag ttccaggcac tcttgtagga tatttaatgt
     1861 taccgacgga gggacgacaa aattgatgat cgtcggtttt acgggtaatg ccgcaagatt
     1921 ggcgtaggct ttatccccct cgacttcggt ggctgaggga ttaacaggaa aaatcgtata
     1981 ccctttacgc cgaagatcac gataaatcgt atggccgtat ttattgggat tattattggc
     2041 ccccacgacg gcgatcgtca cctgcggttc acgcagcaga tcaaggatat ctttcatcgg
     2101 tcactccatt tcttttttca aaagatggtc cgtggatttt acggcacctt gcatgatggc
     2161 gatatccagt atctgcaata agtgtcgcaa ttctttcata tcatccaagt accactgcat
     2221 ccccggtttt aatcgtccgg agcgatcata aaaatcatca tttgtatacg acgggccgtg
     2281 aaaccaccgg cggcattcgt tttgaaaaac atccgatttt ttttcgatca acgtgatcag
     2341 attggaacct tgactgatac tcacgatatg ctcgcgttcg atattttcca aatagtccga
     2401 attgaacgcc gcacgcacta ccagcatcgt ggaagtaaaa tcaatcgtag ggtgaaattt
     2461 taattccggt ttttttccgg cggccatggc cgattcataa aacacaatca acgaatccaa
     2521 aacttgcatg cgcgggataa ccatttgccg atccatgcgc accagatctt cgcgaaacgt
     2581 ctgcagaaat gcgatccgtg cccgtttggc gcgttgctga tcctgaaaat ccgatacgac
     2641 aaaagcgccg tatactccgg caaatacgat gaggagttca aaaattaatc ggcctacgaa
     2701 atgttctttt ttttcttcgg acatgcacag ctcccgattt tgtcgtcaat ctaatggcct
     2761 acgtaacaaa ttacaaacac aaaacgggcg tc
//
LOCUS       DAOQGF010000227         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_23299_length_2715_cov_5.023684, whole genome shotgun sequence.
ACCESSION   DAOQGF010000227 DAOQGF010000000
VERSION     DAOQGF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23299_length_2715_cov_5.023684"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..711
                     /locus_tag="PK937_16125"
     CDS             214..711
                     /locus_tag="PK937_16125"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HNH34151.1"
                     /translation="MRKYTKVIALTLAVLMGSISVSSCYGKFALTRKLYTWNGSVTGN
                     KFVNNLVFWALLIIPVYSIAGFVDAILNTVEFWTGSNPMAMKEGEKEVKQFAHEGNTF
                     EVTATKNRLQIVQLAGEQAGASTVLVFRPESKSWYAETAEGAYKIASITDENNANFVN
                     PFAAK"
     gene            complement(961..1149)
                     /locus_tag="PK937_16130"
     CDS             complement(961..1149)
                     /locus_tag="PK937_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34152.1"
                     /translation="MKKNKKLNRRDTGHIQLSYYHVDCWEKRVDLQSMPINAAVGETM
                     REIPRKEKVKQTEKGVGL"
     gene            complement(1386..1859)
                     /locus_tag="PK937_16135"
     CDS             complement(1386..1859)
                     /locus_tag="PK937_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNH34153.1"
                     /translation="MHEKTTKITDFDELDRKILNLLMENAQMPYTEIAEKVHTSSGTV
                     HVRMRKLESAGVVTGSKLRVDPAMLGYDLCAFLGIFLEKGSLYKKVVKQLEAIPEITE
                     LHYTTGNYNILAKLYCRDTRHLRDVLNDKIQTIEGVERTETFISLEPSLERAIRI"
     gene            complement(2207..>2715)
                     /locus_tag="PK937_16140"
     CDS             complement(2207..>2715)
                     /locus_tag="PK937_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34154.1"
                     /translation="SYPQMNGKTSLHSKKNATEAFLWSAGHTVLPVVAGGYILNKLDT
                     YETAGSFAVGYGFLIGPSMGNFYAQDFGRGLLGVGIRTAGTALFYSGVADALQDDWFD
                     DEGNDSRGDGGAKITGGFIIYTIGALYSIFTLPQSVHDYNKKFELSASPSYDPKSQTL
                     SLHAQLVF"
BASE COUNT          814 a          563 c          521 g          817 t
ORIGIN      
        1 cgaatggatt gtcgaacatg cggcacagat ggcgattgag cgggcaaaaa atcacaaacc
       61 ggggcaaaac aatctcactt ggggtacttt tgtgaaatct tttgcagccg ataaacctct
      121 gggaaaaact gataccgtaa aaaagaagaa aaaataaatt ccgtttatct tatttaatta
      181 accgatttct aacctcacgc aggagtcact aacatgagaa agtacacgaa agtcatcgct
      241 ctcactttgg ctgtattgat gggttctatc agcgttagca gttgttatgg caaatttgca
      301 ttgacccgta aactctatac atggaacggt tccgttacgg gaaataaatt tgtaaacaac
      361 cttgtgtttt gggcgttact tattatccct gtatattcaa ttgccggatt tgttgatgcg
      421 atactcaaca cagtagaatt ctggaccgga agcaatccaa tggcaatgaa ggaaggcgaa
      481 aaggaggtca aacaatttgc acatgaaggc aatacgtttg aagtcacggc cacgaaaaat
      541 cgtcttcaaa tcgttcaatt ggcaggcgaa caagccggcg catccaccgt attagtattc
      601 cgccccgaaa gcaagagctg gtatgctgag accgctgaag gtgcatacaa aatcgccagc
      661 atcacggatg aaaataatgc gaattttgta aacccttttg cagcaaaata atttttaatt
      721 gttcttccgt taaaaaaaac ccgcttctaa aaagcgggtt ttttatttca aaaaaagaaa
      781 ccccgcacat cttggaatgc cggggtttat atctgaggcc ttggcaattt tgatggactg
      841 tcccatagat ccatctattg cctgtgggta atgaggagcg ttattgattt ggccgcaatg
      901 gcccttcatt ggagataggg cgtttctgcg gttttggcgt cagcttcgta ttcgtcggtt
      961 tcatagccct actcctttct ctgtttgttt cactttctct ttacgcggaa tctcgcgcat
     1021 tgtttcgccg acggcagcat tgatcggcat actttgcaag tcaacgcgct tctcccaaca
     1081 atctacatgg taatacgata attggatgtg tccggtatca cgtcggttta attttttatt
     1141 ctttttcata tccgtatcac ctttgctatt tgctatatat gtattagcat agagcgtgcc
     1201 aggtatatga aaatttataa gaatctgttt tttatgaaac gttatgaaat cctgccaaca
     1261 aaaaaatgca ataaatcgaa catttaatat tgcagtgtag ctttttcact acactacaca
     1321 atcagaaatc tgaaaactca atccgtattc cgatacttta tctgtgcgtt ccgtcacatt
     1381 ctatatcaaa tacggatagc tctttctaaa cttggctcta aagaaataaa ggtctcggta
     1441 cgttcgacgc cctcgatagt ttgaatttta tcgtttagta catctctgag atgacgcgtg
     1501 tcccggcaat agagtttggc taaaatatta taattacctg tcgtatagtg aagttcagtg
     1561 atttccggaa tagcctcaag ctgcttcacc acttttttat acaacgagcc tttttcaaga
     1621 aaaattccga gaaacgcgca cagatcataa cccaacatgg ccggatcaac gcgcagtttg
     1681 gatcctgtaa cgacaccggc cgattccaat tttcgcatgc gaacatgtac cgttcccgaa
     1741 gaagtgtgaa ctttttcggc aatttctgtg tagggcatct gcgcattttc catcaaaaga
     1801 tttaatatct ttcgatccag ttcatcaaaa tcggtaattt tagtggtctt ttcgtgcata
     1861 attttacaaa aaattaaaaa aataatatat tttatacaat atatgtaata atatttaaaa
     1921 taaaataaat tatttgtaat aatatacact ttatattaca ttatttacgt taataataga
     1981 ttatttctgt tctttttata attcaaatat tgtagggtga tgaaacaggc agccataccg
     2041 tccggtctcg acggtggaga atttgggata aattccgatt accgggacta actactccgt
     2101 ggaggttcga ctccttcccc tacagctcgt acaccaccat aataaaaaac tgttccgccg
     2161 gtcacggaac agtttttttg ttatcgactt ttggggttaa ttagaattaa aaaacaagtt
     2221 gtgcatgaag cgataaagtc tgggatttag gatcatacga gggactcgct gataattcaa
     2281 attttttgtt ataatcatgg acagattgtg gtaaagtaaa aatgctatac aaagctccga
     2341 tcgtataaat aatgaacccg cctgttattt ttgcgccgcc gtcacctcga ctgtcattac
     2401 cttcgtcatc aaaccaatca tcttgaagcg catccgcaac accactataa aacaaagcag
     2461 taccagcggt gcgaatacca acacctagaa gacctcgccc aaaatcttgc gcataaaaat
     2521 tacccatcga aggtccaatt aaaaaaccgt agcccactgc aaagcttccg gccgtctcat
     2581 acgtgtctaa tttgttaagt atgtatcctc cggcaacaac aggtaatacg gtatgtccgg
     2641 cgctccataa aaaagcttct gtagcatttt ttttggaatg aagcgatgtt tttccattca
     2701 tttgtggata cgaag
//
LOCUS       DAOQGF010000228         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_23556_length_2696_cov_4.964029, whole genome shotgun sequence.
ACCESSION   DAOQGF010000228 DAOQGF010000000
VERSION     DAOQGF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23556_length_2696_cov_4.964029"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1048
                     /locus_tag="PK937_16145"
     CDS             359..1048
                     /locus_tag="PK937_16145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNH34155.1"
                     /translation="MLPKAVADGFTRLLQPLIAFSSRTRINPNWFTTLGLLLNVVAAL
                     IFVAGSMYGTRSDLHYIGWGGFFVLMGGICDILDGKVARSTGLSSRFGALYDSVIDRY
                     SEVIMFLGMGYYLIGHNYFYESVFCFVALGGSTMVSYIRARSEGLQLECKVGLMQRPE
                     RVVWLGFGALFCGLSQYFVSPDFIFSIGSTHVFKPIYMFTGPIVIVAVLSNYTSVQRM
                     VHSYHQSKTSN"
     gene            1089..2405
                     /locus_tag="PK937_16150"
     CDS             1089..2405
                     /locus_tag="PK937_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNH34156.1"
                     /translation="MEKKSLDIKPASGKLGVLLPGMGAVATTMVAGVIAVRKGLAQPV
                     GSLTQLGTIRLGKRTEKRIPKINEFVPLSGLEQIAFGGWDIFEDNAYQAAIKAGVIEK
                     DLLNQIKPELEAVKPMKAVFDQRYVKRLNGTHVKQGANKMELADMLRKDIQDFRKTNG
                     CDRLVMVWCGSTEVFIKPTDVHQTIEAFEKGLRENDINIPSSMIYAYAAIMEGVPYAN
                     GAPNLSADVPALVDLAQKKNVPICGKDFKTGQTLMKTILAPGFKARLLGMNGWYSTNI
                     LGNRDGEVLDDPESFKTKEESKLSVLEHILQPEVYPDLYGKISHVVRINYYPPRGDNK
                     EGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPIVLDLALFLDLASRSGFKGIQEWLSF
                     YFKSPQHATAVYPEHDLFIQLMKLKNTLRWMRGEELITHLGLEYYD"
     gene            complement(2474..>2696)
                     /locus_tag="PK937_16155"
     CDS             complement(2474..>2696)
                     /locus_tag="PK937_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNH34157.1"
                     /translation="KRHKDKWLYGSHGFDPEIKEMGGLFIASGPAFEKGKIIPSFENI
                     HIYALVCHILGITPSANDGDINVFKSAIK"
BASE COUNT          741 a          565 c          593 g          797 t
ORIGIN      
        1 aacgtgatga aaaactttgg ttgcccgagg ggcatcatcg caatttatat gaatttgaat
       61 tatatcgcac atcggttacc aattttttga cccgatatgc aaaaaccaat ttatacacat
      121 taatggctgg agagaaaaca tatgcatctt tgccttaagc gctggttaaa acgctggttt
      181 ggcaatatct tcagttggtc tttttcgcag gatgatattg aagacggatt acctaatctg
      241 cattggcgta cgccgaaaag ctgagttttt agcttgattt tttaggcttc gatcaatact
      301 ttgggcgcgt tatttgaaca ttatccgcac ttcaaattct gaggtttttt tgagttttat
      361 gcttcccaaa gcggtggccg acggctttac acgccttttg cagccattga ttgcattttc
      421 aagccgcacc cgaattaatc ccaattggtt caccacatta ggacttttgc tcaatgtagt
      481 tgctgcactt atattcgtag ccggatcaat gtacggaaca cgaagtgatt tacattatat
      541 tgggtggggc ggatttttcg ttttgatggg cggtatttgc gatatcttgg atggcaaagt
      601 ggcccgctct acggggcttt catcgcgatt tggtgcattg tatgattcag tgattgatcg
      661 ctatagtgaa gtgatcatgt ttctcggtat gggatattat ctgatcggac acaattattt
      721 ttatgaatca gttttttgtt ttgtagcttt gggtggttcg acgatggtca gctacatccg
      781 tgcacgatcg gaaggtttgc aacttgaatg taaagtcgga cttatgcaac ggcccgaacg
      841 tgtcgtttgg ttaggttttg gcgctttatt ttgcggcctt tctcaatact ttgtttcacc
      901 ggattttatt ttttctattg ggagtacaca cgtttttaaa cccatttata tgttcaccgg
      961 ccctatcgtc atagtcgccg tgttgtccaa ttatacgtcc gtacaacgta tggttcacag
     1021 ttatcatcaa agcaaaacat caaactgaat ttgatatttg ttttagcata ataagaagga
     1081 gtcttgcatt ggaaaaaaag tctttggata tcaagcccgc atcgggaaaa ctgggcgtct
     1141 tattaccggg catgggcgct gtggccacga cgatggtcgc gggcgtcatt gccgttcgta
     1201 aaggtctcgc tcaaccagtc ggatcactga cccaacttgg aacgatacgc ctcggcaaac
     1261 gcaccgaaaa aagaatacct aaaatcaacg agtttgtacc gcttagcggt ttggaacaga
     1321 tcgctttcgg cggttgggat atttttgaag acaacgccta tcaagcagcg atcaaagcgg
     1381 gtgttatcga aaaagatttg ctcaatcaga tcaaaccgga attagaagcg gtaaaaccga
     1441 tgaaagccgt ttttgatcag cgttatgtca aacgtctcaa cggtacgcat gtcaaacaag
     1501 gcgccaataa aatggaatta gccgacatgc ttcgtaaaga tatccaagac tttagaaaaa
     1561 caaacgggtg tgatcgtctc gtaatggtat ggtgcggttc tacagaagtt tttatcaagc
     1621 ctaccgatgt acatcagacc atcgaagcgt ttgaaaaagg attacgagaa aacgatatca
     1681 atataccctc cagcatgata tacgcctacg cggcgatcat ggaaggcgtc ccttatgcca
     1741 atggcgcccc caatctatca gctgatgttc cggcattggt ggatcttgca cagaaaaaaa
     1801 atgttcccat ctgtggaaaa gatttcaaaa ccggtcaaac gctgatgaag accattcttg
     1861 caccaggatt caaagcccgt ctgcttggaa tgaacggctg gtattcaaca aatattctcg
     1921 gaaatcgcga cggcgaagta cttgacgatc cggaatcctt caaaactaaa gaagaaagta
     1981 aattatccgt acttgagcac attttgcaac cggaagtgta tccggatctt tacggaaaaa
     2041 tttcacacgt tgtacgaatc aactattatc ccccacgcgg cgataataaa gaaggatggg
     2101 acaacattga tatctttggc tggctcggat atccgatgca aatcaaagtg gactttctgt
     2161 gccgtgactc catattagca gcgcccatcg tcttagacct tgctttattc cttgatctgg
     2221 catctcgctc cggattcaaa ggtattcaag aatggctttc gttttatttt aaaagtcctc
     2281 aacacgccac ggcagtttat ccagaacatg atctctttat ccaactgatg aaactcaaaa
     2341 acacattgcg ttggatgcgc ggcgaagaac tcatcacgca tttgggattg gaatattacg
     2401 attaatcgaa gattgttaac atcaaaaaag ggagcgttta gcgctccctt ttttatttct
     2461 atcgtctgag atttcacttt atcgccgact taaatacgtt gatatcgccg tcattggccg
     2521 aaggcgttat cccgagtata tgacatacca atgcatagat atggatgttt tcaaatgatg
     2581 gtataatttt ccctttttca aatgcaggac cagacgcgat aaataagccg cccatctctt
     2641 tgatttcagg atcaaaacca tgagagccat aaagccactt gtctttatgc cgtttg
//
LOCUS       DAOQGF010000229         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_24198_length_2653_cov_4.144342, whole genome shotgun sequence.
ACCESSION   DAOQGF010000229 DAOQGF010000000
VERSION     DAOQGF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24198_length_2653_cov_4.144342"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PK937_16160"
     CDS             <1..237
                     /locus_tag="PK937_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34158.1"
                     /translation="ANGFQSLVSRSSGIRFLVDAHKPEFNRLRFFIIVIFWREKIDIA
                     NEVHLFCCLMRFKHLQALSIKRKIDLNFFGINVL"
     gene            324..2198
                     /locus_tag="PK937_16165"
     CDS             324..2198
                     /locus_tag="PK937_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNH34159.1"
                     /translation="MNDLLSTEVQTAESYIESASRYAQRTQDAPSAITIITQKDIQRY
                     GYQTLAQLLSAQVGFYITYDHNYSYIGIRGFSRHTDYNNRILLLIDGHRVNDYFYDQA
                     MLGPEFGFDLQGFERVEIIRGPGSALYGTSAMFAVINLITRNHFEKPSVYASYGSYGN
                     LQTAIHFSPLKTESTTWSVSANLLNSRGHSYRFDELALDSVTGGLMRNRDEEIAYGVL
                     SKFKYRQMTALVGAKSRLKEVPTASFGTIPSGKLETRDDQLFSDIRWSKALRYNLNVS
                     TRVYGDYYVFHGDYPYSDGLEKDRNINVSVGSEVQAVWDVRPNDRLISGIDFNRNFRS
                     DYRVTYGGEKTFDQNFPYTVVSIYLQNEWQIFSSLLLCAGLRRDEYVNRNHAYSPRGA
                     VVYTPVKTHTFKLLYGEAFRNPNNNEQHIDPVDAGEQEAKGLKPERLYSLEGIWNYQL
                     SSSLLSSISLYRFRIKGIIDQVEVMPDSSITTNTDAYKALGAEWELTGSWDNDNSGYL
                     RYSFQQARNENSNRRLTNSPLHLVKAGYSRAFMKFFNGTAEVQYESSRRTLSDARTKN
                     VILLHTGLDYKTGSHIRISLKVLNMLNRRYEHPAGAEHVQDIFLQSRRTFVLSAGYNF
                     "
     gene            2212..>2653
                     /locus_tag="PK937_16170"
     CDS             2212..>2653
                     /locus_tag="PK937_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34160.1"
                     /translation="MSNRNTKLWLGFLMLVIPALQAQVVKVPADIQMRLMVKILAYER
                     DHRFDSDNFTNVVVLYHSKVSGSVAAKDAVINAHTQSPLKVNRAIIRFIPIDISTVGD
                     IGEELHRQGARVVYLTPMRGVNIGAIINACRAQSILSVSGVYDFA"
BASE COUNT          723 a          509 c          619 g          802 t
ORIGIN      
        1 gcaaatggtt ttcaatcatt agtttcccgc tctagcggga ttcgattcct tgtggacgca
       61 cacaaacctg agttcaacag actcaggttt tttattatcg taatattttg gagagaaaaa
      121 atcgatattg ctaatgaagt tcatttattt tgttgcctga tgcggtttaa acacttgcaa
      181 gcgttatcaa ttaaacgtaa aatagatttg aatttttttg ggataaatgt tttatgaagt
      241 tgatggttta tatttgtgtc tgtgcgtttg ttatttggag ttcaatttat tcccaaacgc
      301 caattgacac ttcggctcaa ggcttgaatg atcttctttc aaccgaagtg caaaccgctg
      361 agagttacat agaatcagcg tcccgttatg cgcagcgtac gcaagatgcg ccttctgcta
      421 ttaccataat tacgcagaag gatattcaga ggtacggcta ccagacactc gcccaactgc
      481 tgagtgctca ggtcggtttt tatatcactt acgaccataa ctacagttat atcggtattc
      541 gcggtttcag ccggcacacg gattataata atcgaatttt gttgttaatt gatggtcata
      601 gggtcaatga ttatttctat gatcaggcga tgcttgggcc ggagttcgga tttgacttac
      661 aaggctttga acgtgtagaa attattcgcg gcccgggctc agcactttac ggaaccagcg
      721 ccatgtttgc tgttattaat ctgattacac gaaatcactt tgagaaacca tcggtttatg
      781 catcgtatgg ttcatacggg aacctccaaa cggcaattca cttttctcct ctaaagacgg
      841 aatcaacgac atggagtgta tcggcgaact tactgaatag cagaggccac agctaccgtt
      901 ttgatgaatt ggcgttagat tctgtgaccg gaggattgat gagaaatcga gatgaagaaa
      961 ttgcatacgg ggtactttcc aaattcaaat accggcaaat gacagcgctt gtcggagcta
     1021 aatctcgttt gaaagaagta cctaccgctt catttggcac gatcccatcc ggtaaacttg
     1081 aaacccgtga tgatcagctt ttttcagata tacgttggtc taaggcgttg cgctataatc
     1141 ttaatgttag cacacgtgtg tatggagatt attacgtttt tcacggagat tacccttaca
     1201 gtgacggttt ggaaaaggat cgtaacatca atgtctctgt cgggagtgaa gtacaagcag
     1261 tgtgggatgt acggcctaat gatcgcttaa tttcgggaat tgattttaac cgtaattttc
     1321 gctccgatta ccgagtgacg tacggcggtg aaaaaacgtt cgatcaaaat tttccatata
     1381 ccgtagtttc gatatatctg caaaatgagt ggcagatatt ttcaagcctt ttgttgtgtg
     1441 ccggattaag gcgcgatgag tacgtgaacc gcaatcatgc gtatagccct cgcggggcgg
     1501 tggtttatac gccggttaaa acgcatacat tcaaattact ctacggtgaa gcgtttcgaa
     1561 accccaataa taatgaacag catattgatc ctgttgatgc gggtgaacag gaggcgaaag
     1621 gattaaagcc tgaacgtttg tattctttag aaggcatctg gaattatcag ttatcttctt
     1681 cattattgag ttcgatttcg ttataccgtt ttcgcatcaa aggaattatt gatcaggttg
     1741 aagtaatgcc ggattcttcc attacgacca acacggatgc gtataaggcg ttaggtgcgg
     1801 agtgggaact aaccggcagt tgggataatg ataacagcgg ttatcttcgt tattcgtttc
     1861 aacaagcgcg taacgagaat agcaaccgac gattgacgaa ttctccgctg catcttgtaa
     1921 aagcggggta ttcgcgagca ttcatgaagt tttttaacgg tacagcggag gttcagtatg
     1981 aatcatcacg ccggacttta tcggatgcac gaacaaagaa tgttattctg ttacatactg
     2041 gtttggacta caaaaccgga tcgcatattc gtatatcttt gaaagtgtta aatatgttaa
     2101 accgtcggta tgaacatcct gccggagcag agcatgttca ggatattttt ctacaaagcc
     2161 gtcggacatt cgtattgtct gccggttata atttttaatc ggataaaata aatgagtaac
     2221 agaaatacca aattgtggtt ggggtttttg atgttagtta ttccggcact tcaagcgcag
     2281 gttgtcaaag taccagccga cattcagatg cgcctgatgg tcaaaatcct tgcctatgag
     2341 cgagatcacc ggtttgattc cgataatttt accaatgtcg ttgttcttta tcatagtaaa
     2401 gttagcggtt cggttgcggc gaaggatgcc gtgatcaatg ctcatacgca gtctccgcta
     2461 aaagtcaatc gggcgattat tcgttttatt ccgatagata taagtacggt gggtgatata
     2521 ggagaggaac ttcatcgcca aggtgcgcgt gttgtctatt tgacgccgat gcgcggagtt
     2581 aatataggag ctattataaa cgcatgccgc gctcaaagta tattatcagt cagcggggtc
     2641 tatgattttg cac
//
LOCUS       DAOQGF010000230         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_24753_length_2615_cov_3.969141, whole genome shotgun sequence.
ACCESSION   DAOQGF010000230 DAOQGF010000000
VERSION     DAOQGF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24753_length_2615_cov_3.969141"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2615)
                     /locus_tag="PK937_16175"
     CDS             complement(<1..>2615)
                     /locus_tag="PK937_16175"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNH34161.1"
                     /translation="YTLKDGLSQNLVTCALQTRDGFMWFGTREGLNRFDGYEFLVFQH
                     DESDSFSISHNYVTALLEDQDGRIWVGTEDGLNLFDPITGRFEKFFHDPMQPASISNN
                     NINVLFQDSFGTLWIGTHVGLNKLRQSDEPNFERVLTISENKTSLPSNLIRAIGQVHN
                     AGGSAEKKLWIGTTGGGLSVLDMETNTFSKPYDDLIVSKKFNMTTVWDVYEDPADRGN
                     TLWVGTPSGLGKIDRATRQLHFFSFDNKNPNGISHNRVRRILRDSYGRLWVGTTEGGI
                     NLMTGDTFLNFRNTPNQPSLFTDEYIYDIYQSSDGIVWACTYSNGVTQISGMEPRFVH
                     YKKNPLDSHSLIHNVVRSFTESGDGSIWISTDDGISRWDRNTDRFEHLRFIPDNPNAL
                     SDRQIHRVYEDRSGKIWIGTHQSGLQCYDPVRKTMKSFRRSTTQGQFAAIRVYDILED
                     ENGYIWLATYRSGLIRYDPQNDQFKTYGLKKAGGQNIRKEMLTVLHRDNAGKLWIGTQ
                     QRGLLCFDRQSEQFTTFSKNTAEAFTISDNHVYSIYQDKSGYLWIGTANGLNRFDPLT
                     QKFQRITRKDGLPNNVIYGIIGDEHDNLWLSSNRGIIRMQTKSDTSRVFTVYTSDDGL
                     QADQFEPNACLRTREGEFLFGGVNGFNIFHPRAIVPHGQAPRLALTDFRLFDKPTKLD
                     TSIPYKHDLHLNYDQNFFSFSFSALNFFVPEKNQYAYKLDGIDENWNFVTGKNRIVKY
                     TDIDPGNYTLLVRASNHDQIWNNDGLRFPVVIRPPYWETWWFRTILFATLVGILVFAY
                     TARVRNIEAMNRRLEGIVIERTAALNQKKDELQQAHDELEKRVEERTKQLRDLSDHLV
                     RVREEERTRISREVH"
BASE COUNT          706 a          589 c          573 g          747 t
ORIGIN      
        1 atgcacttcg cgcgaaatcc gcgtacgctc ttcttcacgg acccgaacca agtgatcgga
       61 gagatcgcgc agctgtttag tgcgctcttc gaccctcttt tccaattcgt catgcgcctg
      121 ctgcaattcg tctttcttct gatttaaagc tgccgttcgt tcaataacga taccttcaag
      181 tctgcggttc attgcttcaa tattgcgtac acgtgcagta taagcaaata ctaaaattcc
      241 gaccaacgta gcaaataata tcgttcggaa ccaccaagtt tcccaatatg gggggcggat
      301 cacaaccgga aaacgaagac cgtcgttatt ccaaatctga tcgtgattgg aagcgcggac
      361 cagaagcgtg taatttcccg gatcaatgtc cgtatatttt acgatgcgat tttttcctgt
      421 tacaaaattc caattttcat caataccatc cagtttgtag gcgtattgat ttttttcggg
      481 gacaaaaaaa ttgagtgcgg aaaatgaaaa actaaaaaaa ttctgatcat aatttaaatg
      541 aagatcgtgt ttgtaaggaa tacttgtatc caatttagtg ggtttgtcaa aaagcctgaa
      601 atccgtaagc gctagtcgcg gtgcttgtcc gtgaggtacg atagcgcgcg gatgaaagat
      661 attaaatcca ttaacaccgc caaaaagaaa ttcgccttca cgcgtgcgaa ggcaagcatt
      721 cggttcaaat tgatcagcct gtaaaccgtc atcggatgta taaacagtaa aaacgcggga
      781 agtatcggat tttgtttgca tccgaataat accgcgatta ctgctcagcc acagattgtc
      841 atgttcatcg ccgataatac cgtaaatgac attgttaggc aaaccgtctt tacgggtgat
      901 gcgttgaaat ttctgtgtaa gtggatcaaa gcgatttaac ccgttggccg tgccgatcca
      961 tagataacct gatttatcct gatagatgct atatacgtgg ttatctgaaa tcgtgaaggc
     1021 ttccgccgtg tttttggaga atgtcgtaaa ctgttcggat tgccgatcaa aacacagcag
     1081 tcctctttgt tgtgtaccga tccataattt cccggcgttg tctctatgga gtacagttaa
     1141 catttcttta cggatattct ggccgcctgc ttttttaagc ccataggtct taaattgatc
     1201 gttttgtgga tcatatcgaa taagtccgct gcgataggtt gcaagccaaa tatatccatt
     1261 ttcgtcttcg agaatgtcgt atacgcgaat ggcagcaaat tgaccttgtg tagtcgagcg
     1321 ccgaaaggac ttcattgttt tacgtaccgg atcataacat tgcaagccgc tctgatgcgt
     1381 accgatccat attttacctg aacgatcttc gtaaacgcgg tgaatctggc ggtcacttaa
     1441 agcgtttgga ttatccggta taaaacgcaa gtgttcaaag cgatctgtat ttctgtccca
     1501 tcgtgagatg ccgtcatcgg tgctgatcca aatcgaaccg tcaccactct ccgtgaaact
     1561 gcgtactaca ttatgtataa ggctgtgcga gtccaacggg ttttttttgt agtgaacaaa
     1621 acgcggttcc attccgctga tttgtgttac tccattggaa tacgtacaag cccatacaat
     1681 gccgtcggaa gattggtaaa tatcgtagat atactcatcg gtaaaaagag aaggctgatt
     1741 gggcgtattg cggaaattta gaaacgtgtc gccggtcatc agattgatgc cgccttcggt
     1801 cgtccctacc cacaagcgcc catatgagtc gcgcagaatg cggcgcacac gattatggct
     1861 tataccgttc gggtttttgt tatcaaaaga aaaaaaatgc aattgccttg tcgcccggtc
     1921 tatttttcct aaacctgacg gcgtacctac ccacaaggtg ttgccccggt ctgccggatc
     1981 ttcatacaca tcccaaactg tggtcatatt gaatttcttc gatacgatca gatcgtcata
     2041 aggttttgaa aatgtattgg tttccatatc caatacactg agaccgccgc cggtggtacc
     2101 gatccaaagt tttttttctg ccgagccacc tgcattatga acctgaccaa tcgcccgaat
     2161 taaattggac ggtaaacttg ttttgttctc ggaaatggtt aaaactcgtt caaaattcgg
     2221 ttcatccgac tggcgtaatt tattaagtcc gacgtgggta ccgatccaca atgtcccaaa
     2281 actatcctga aacaacacat tgatattatt attgctgatg gatgccggtt gcatcgggtc
     2341 atgaaaaaac ttttcgaagc ggcctgtgat ggggtcaaaa aggttgagtc cgtcttccgt
     2401 tccgacccaa atacgtccgt cttgatcttc gagcaaagcc gttacataat tgtgagaaat
     2461 ggaaaagctg tcactttcgt cgtgctgaaa aacaaggaac tcatacccgt caaagcgatt
     2521 gaggccttcg cgtgtaccaa accacataaa tccgtcacga gtctgaagcg cgcaggttac
     2581 cagattttga gataatccat ctttgagcgt atagg
//
LOCUS       DAOQGF010000231         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_25028_length_2599_cov_6.974450, whole genome shotgun sequence.
ACCESSION   DAOQGF010000231 DAOQGF010000000
VERSION     DAOQGF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25028_length_2599_cov_6.974450"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1612)
                     /locus_tag="PK937_16180"
     CDS             complement(110..1612)
                     /locus_tag="PK937_16180"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNH34162.1"
                     /translation="MDSIFWQVTLSDGIGYALAFLSLGVILATVELLRRRFFWPPEIT
                     RKITHIGVGILIFFAPSWFDSGAPAVYIGVFFVVFNMLAIRGHWLQGIHGTDRRSYGT
                     VYYPMAFTMLVLLCWETAPLVLMSAMLVLALGDASAGLVGQLWPSPRHYYLTSDRKSI
                     EGSITMFVVSTLVIAFSFLYYQNAHIAEFSKFTIICAALIGALLATIMEAISPAGSDN
                     LTIPLTVAWVVYTITHQTEEATVYLIYGVGLAACVGWASFRFRLLTADGAAGAFLLGS
                     VIFGIGGWMWALPILVFFISSSLLSRLGKRRKAQFDTIFEKGSTRDAGQVLANGGLAG
                     AVALAAQLYPGEVWYTVYLAVIAAATADTWATEIGTFFQATPRSVLHWRKVQPGTSGG
                     ISLPGTLGAFTGSLWIAMTGAYVMGENLNIQGLWFVTLAGFLGSIADSFLGATLQAQY
                     ACPTCGKPTEKKQHCDEPTRQVRGLVWMHNDAVNMIASGFGGILAYIMMT"
     gene            complement(1778..2467)
                     /locus_tag="PK937_16185"
     CDS             complement(1778..2467)
                     /locus_tag="PK937_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34163.1"
                     /translation="MKPIRSWIVLIFVLMSGWSLQGQNEEQTDETNSNRKKIGIGISF
                     GTNTHRFGDDDDYGYFKTGAIMGIYVPINFGDRWRIEPEVSYNNMSGKISRSDDDYYY
                     KSNSTVKISRYGVGILRRKKRDHTETYFGARLGKVFIEDKTRFTYSAPGYEDGDTDKR
                     HKSDLYFGPVAGGEYYFSPYFSFGGEAQLIYTKVGRMYGDEYEDDPDEDISYNVHAWD
                     TRAHLFVRWYF"
BASE COUNT          772 a          686 c          512 g          629 t
ORIGIN      
        1 caccatcaga aaacgtaacg actttacatt ttctactttt ttagtcgccc aaacattacc
       61 taccacgcgt ccgatgaaca tgcgtcaatc cgttgattaa tgaaaatttt taagtcatca
      121 ttatataagc caatattccg ccgaaaccgc ttgcgatcat attcacggca tcattgtgca
      181 tccataccaa accgcgcact tgacgtgtgg gttcatcaca atgttgtttt ttctctgtag
      241 gctttccgca cgttgggcaa gcgtactggg cctgcagcgt cgcacccaaa aaactgtcgg
      301 ctatacttcc caaaaaacct gccaacgtaa caaaccacaa accttgtata ttcaggtttt
      361 cacccattac gtatgcaccg gtcatcgcga tccacagcga accggtgaaa gcgcccagcg
      421 taccaggcaa tgaaattccg ccggaagttc cgggttgtac tttacgccaa tgtaaaacac
      481 tgcgcggggt tgcttgaaaa aaagtaccga tttcggtagc ccaggtatcc gccgtcgccg
      541 cagcaatcac ggccaaataa acggtatacc aaacttcacc gggatataac tgagcggcta
      601 atgcaacagc cccggccaag ccgccattgg ccagtacctg tccggcatca cgggtactgc
      661 ctttttcaaa gatcgtatca aactgcgctt tacggcgttt gcccaagcgc gacaataaac
      721 tggatgatat aaaaaacaca agaatcggta aagcccacat ccatccgccg attccaaaaa
      781 tcacactgcc caacagaaac gcacctgccg cgccgtcggc cgtcaataag cgaaagcgaa
      841 acgatgccca gcctacgcaa gctgccaaac ccacgccgta tatcaaatat acggttgctt
      901 cttccgtttg atgcgtgatc gtatatacga cccacgccac cgtcagcgga atagtcagat
      961 tatccgagcc ggcgggtgat atggcttcca tgatcgtcgc caaaagcgct ccgatcaatg
     1021 cggcacagat gatcgtaaat ttcgagaatt ccgcaatgtg agcattttga taatacaaaa
     1081 aagaaaaagc aatcacaagc gttgatacga caaacatagt gatcgagcct tcgatgcttt
     1141 ttcggtcgga ggtcagataa taatgacgcg gtgaaggcca taattgccct actaagccgg
     1201 cggatgcatc accgagcgct aaaaccaaca tggctgacat taaaactaac ggggccgtct
     1261 cccaacacag cagtaccaac atcgtgaaag ccatcggata atatacggtc ccatacgacc
     1321 ggcgatccgt tccgtgaatt ccctgcaacc aatgcccgcg aatcgcgagc atattaaaca
     1381 caacaaaaaa aacgccgata tacacggccg gcgctcccga atcgaaccag gaaggcgcaa
     1441 aaaaaatcag aatgccgacg ccaatatggg ttattttacg cgtgatttcc ggtggccaaa
     1501 aaaaccgacg acgtaataat tccaccgtcg ccaagatgac acctaacgac aaaaaggcca
     1561 gtgcatagcc gatcccatca ctcagagtca cttgccaaaa aattgaatcc ataatcttct
     1621 aaacagggag aggataaatg cgtttagtat caatacgcta agacgtgata ataaccgatt
     1681 gggtcttatt aaaaacttct aaccgaattc gttcgtacta ctaggaatgc aaacctatta
     1741 attgtatgcg cagtgaagac ggcatgttta ttgaaattta aaaataccag cgtacaaata
     1801 aatgcgcacg ggtatcccat gcatggacat tataactgat atcttcgtcc ggatcatctt
     1861 cgtattcatc accgtacata cgaccaactt tagtatagat caactgtgct tcgccgccaa
     1921 aactgaaata cggtgaaaaa taatactcac cgccggccac cggtccaaaa tacaaatctg
     1981 atttgtgtct tttatcggta tcaccgtctt catatccggg cgccgaatag gtgaatcgtg
     2041 ttttatcttc gatgaacact ttgcccaaac gtgcgccaaa ataagtttcc gtatgatcac
     2101 gtttttttcg acgtaaaatc ccaacgccat agcgggatat ttttaccgtt gagttagact
     2161 tgtaataata atcatcatcg cttctactga ttttaccgga catgttgtta taactcactt
     2221 ccggttcgat acgccatcgg tcaccaaaat tgatcggcac ataaatcccc ataatggcgc
     2281 cggttttgaa ataaccataa tcatcgtcat cgccaaaccg gtgtgtattc gtgccgaacg
     2341 aaattccgat cccaattttt tttcggttgg aattagtttc gtctgtttgc tcttcgtttt
     2401 gaccttggag cgaccatccg ctcatcaata caaaaatcaa aacgatccag ctgcgtatcg
     2461 gtttcattta ttctcccctt ttttaataca gaatagagcc cccgtaagaa aaactacacg
     2521 gccttcaacg cattttcgat atccgcgatc aaatcttctt tgtcttcgca acccacggac
     2581 aaacggacca aaccgtcgg
//
LOCUS       DAOQGF010000232         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_26047_length_2538_cov_5.968586, whole genome shotgun sequence.
ACCESSION   DAOQGF010000232 DAOQGF010000000
VERSION     DAOQGF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26047_length_2538_cov_5.968586"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK937_16190"
     CDS             <1..467
                     /locus_tag="PK937_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34164.1"
                     /translation="MYTQAQTISEGPIIFLDTVDGGKYKNRKLLDEELKSIGGKMDFL
                     QNHFRFFTEFNDTLFVIDKINHLRKSDHYWVLNRRDAHHKAWILGWMYLRNDTLGFMY
                     PTRADSLKITKHVSSNNIRIRNDGFIELHVSDSAFKQLLDLDWNNRRFYVRQ"
     gene            528..>1234
                     /locus_tag="PK937_16195"
     CDS             528..>1234
                     /locus_tag="PK937_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH34165.1"
                     /translation="MIKLFYNGKIYTGEKSQPFCEAIVVSGGTIVASGTNEEMRSAFP
                     FAQRISLDGKPVLPAFTDSHTHFLSHCLQQAQIDLSGLTSVGACLNVIGQKAATTPEG
                     TWIKGKGWNQNLWSPAHYPTKEQLDSVTRQHPIALDARDFHSLWVNSLALAQAGINEQ
                     TVFNGAGEICKNENGQLTGIIKEDARTLVWKIMPEDTAEQRAEVLRNLQTLAYKNGLA
                     GVHCMETMPDFEAYQSL"
     assembly_gap    1235..1334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1335..1948
                     /locus_tag="PK937_16200"
     CDS             <1335..1948
                     /locus_tag="PK937_16200"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNH34166.1"
                     /translation="EYDIACAIHAIGDRANRKVLNAFEKIREMFPDKKLRQRIEHAQI
                     IHPDDIQRFARSGTIASMQAIHIPEDIDAANRYWGARARWAYPFRSLLQSGATIALGS
                     DVPIETCNVFEGMTAALCRTRRTSDEVWYPEECMTREEIIYAYTVGAALASGEEHLKG
                     TLSSGKLADFMVLSQDIFSVQPEKIAGTKVQSMVIGGNVVYES"
     gene            1958..>2538
                     /gene="lpxA"
                     /locus_tag="PK937_16205"
     CDS             1958..>2538
                     /gene="lpxA"
                     /locus_tag="PK937_16205"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNH34167.1"
                     /translation="MIHPTALVSKSLDTSIQVGAYSIIDDGSQIGKHTIIHNHVRVTS
                     FTVIGQGVVIHQGAALGGDPQDLKFGGEYSTVEIGDETVIREYVTINRGTGTNGKTKI
                     GSHSLIMAYAHVAHDCTIGDHVIISNAVNMAGHVHIEDYAGIGGMVPIHQFCRIGRYA
                     FVGGGFRVNQDVPPYVLAAGEPLRFSGLNYVGLR"
BASE COUNT          694 a          500 c          582 g          662 t
ORIGIN      
        1 tcatgtacac gcaagcgcaa acaatttccg aaggtccgat tattttttta gataccgtag
       61 atggagggaa atataagaat agaaaattat tggatgagga attgaaaagc atcggcggga
      121 aaatggattt tttacaaaac cactttcgtt tttttacaga gtttaacgat acattatttg
      181 ttatagataa gatcaatcat ttgcgcaaat ccgatcatta ttgggtactc aatcgtaggg
      241 atgcccatca taaagcatgg atactgggtt ggatgtacct tcgcaatgat actttaggtt
      301 ttatgtatcc gacgcgtgcc gatagcttaa aaatcaccaa acatgtgagt tcaaacaata
      361 ttcgtattcg caatgacggg tttatagagc tacacgtatc cgatagcgca ttcaaacaac
      421 ttcttgattt ggattggaat aatcgaagat tctatgtacg gcagtaggat aaattccttc
      481 ttcataatcc cattgtagct taacgaatat ccgctatatt tcttcatatg ataaaattat
      541 tttataacgg aaaaatatac accggcgaaa agagtcaacc tttctgtgaa gccattgttg
      601 tcagcggcgg aacgatcgtc gcatccggta cgaacgaaga gatgcgttcg gcttttcctt
      661 ttgcacagcg catttcattg gacggaaagc cggttttacc tgcttttacc gatagccaca
      721 ctcatttttt gtcacactgc ttacaacaag cacaaattga tttgagtggt ttgacatcgg
      781 tcggagcctg cctgaatgtc atagggcaaa aagcagcgac cactcctgag ggaacttgga
      841 tcaagggcaa gggttggaat caaaatctat ggtcacctgc acactatccg acaaaagaac
      901 aacttgatag tgttacccgt cagcatccca tcgcactaga tgcccgcgac tttcattcgc
      961 tgtgggtcaa ttcgcttgca ctagcccaag ccggtatcaa cgaacagaca gtatttaatg
     1021 gtgccggaga aatatgtaaa aatgaaaatg ggcagcttac aggtattatc aaagaagatg
     1081 cgcgcacatt ggtatggaag atcatgccgg aagatacggc cgagcaacgt gcagaggtat
     1141 tacgcaattt gcagacattg gcatataaaa acggtttggc cggtgtgcat tgcatggaaa
     1201 ccatgcctga ttttgaagcc taccaatctt tgcannnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnctgaat atgacatagc atgcgcgatt catgccatcg gagacagagc
     1381 gaatcgcaag gtgttgaatg catttgaaaa aatacgcgag atgttcccgg ataaaaaatt
     1441 aaggcaacga atcgaacatg cacaaatcat tcatcctgat gatatacaac gtttcgcccg
     1501 gagtggaacc atagcttcga tgcaggctat acacatacct gaagatattg atgcggccaa
     1561 ccggtattgg ggcgcccgtg ctcgatgggc atatcctttt cgtagtttgc tccaaagcgg
     1621 cgcaacgatt gccttaggct ctgatgtacc gatcgaaacg tgcaatgtat tcgaaggtat
     1681 gacagccgct ttatgccgaa cccgtaggac ttccgatgag gtgtggtacc cggaagaatg
     1741 catgacgcgt gaagaaatta tttatgccta taccgtagga gccgcattgg ccagcgggga
     1801 agaacatctc aaaggtacgt tgtcgtccgg taaactggct gattttatgg tgctgtcgca
     1861 ggatattttt tccgtacagc ctgagaaaat tgccggcacc aaagtacaat ctatggtgat
     1921 cggtggtaac gtcgtatatg aaagttgatt tcttgatatg atacatccta cagctctggt
     1981 ttccaaatcg ttagatacat ccatacaagt cggcgcgtat tcgatcattg atgatggctc
     2041 acaaatcgga aagcatacca taattcataa tcatgtacgt gttacatcat ttacggtgat
     2101 tggtcagggc gtagtaattc atcagggtgc ggcacttggt ggtgatcccc aagaccttaa
     2161 attcggtggg gagtattcaa cggtagaaat cggtgatgaa accgtgattc gcgagtatgt
     2221 aacgattaat cgcggaaccg gcacaaatgg aaaaacaaaa atcggttcac attctttgat
     2281 tatggcgtat gcgcatgttg cgcatgattg cacgatcggc gatcatgtta ttatttccaa
     2341 tgccgtaaac atggcggggc acgtacatat cgaagattat gcgggtatcg gagggatggt
     2401 acccatacat caattttgtc gtatcggtcg ttatgcgttt gtcggcgggg gatttcgggt
     2461 caatcaggac gtcccgccct atgtgttggc tgccggggaa ccgttacgtt tttccggttt
     2521 aaactatgtc ggcttacg
//
LOCUS       DAOQGF010000233         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_26554_length_2507_cov_8.121941, whole genome shotgun sequence.
ACCESSION   DAOQGF010000233 DAOQGF010000000
VERSION     DAOQGF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26554_length_2507_cov_8.121941"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK937_16210"
     CDS             <1..561
                     /locus_tag="PK937_16210"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNH34168.1"
                     /translation="SGAFAAIEDAMSTIFGVRGRNYFVSRLVEMGMVVLIGCLFLLSS
                     LISGIIHWLQENHITFFQIDFSNLPYVWDIITNALPYVLIVLTFYVTYKILPKTEIYD
                     RAAMLGALVASVLLELTLYGFSYYVQNFARYNVFYGSVAGVVISVFTIYLASIILLVG
                     AEITEIANTRLENERELAQTIREFES"
     gene            558..>2507
                     /locus_tag="PK937_16215"
     CDS             558..>2507
                     /locus_tag="PK937_16215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNH34169.1"
                     /translation="MTSSSLPVLHPRYDLIEKIGEGGVGIVYRAWDKIDRSTVVIKQA
                     PASTINDTLRDAYWREFEWMFRAHHPGMMKALAWEKSEDGGFFFTMQLAAGIDLFQKI
                     KDRGFWTKKRFDSFIRQIVSALSYVHAHGMVHGDIKPENILIESAGDDCKVTLIDFGF
                     IHAWSNPPVSRDHQGTLAYTAPEILKKEPYDQRSDAYALGVVLYELATGINPFADENV
                     VNIVVHHLQKNITHVDSDFEWWSQDYEKIIVSLLSKDPRQRYPRLEDAFKKLTDQNAV
                     LPPLLCHRFAEPRSVWPDISAQLQTDHPTSVVLRAGEGLGATTMLRELYVRAIHSGYS
                     AQYIRLDQAASLWEVLSGFVPASVLETLSDTDRAIEEILKTIAPNEIYILDQVRLDVP
                     GVMDFLSRFTYTLSSNSGSVILSTTELQTEISFPAKTYVLKNLEPQDVKHFLSTWLFD
                     EDVDEKLVSRMTTWTNGHPGLLYEATRELMEKRQLVFEERSWRLTPTAEWKPSSDWSE
                     KWEAKIKALTDDEIRLIRWMAVADNEIPVLTLTALMENAGAEQNIPRLELFGWIRRKE
                     NAVKIVAPGLRAFLIQKETEAERRAMHDKLLSWYEKNAADAVPQLAYHAFEGNNQEKS
                     VPYKLQWAEREVSRWNYAEALRQYEN"
BASE COUNT          644 a          534 c          633 g          696 t
ORIGIN      
        1 agtggtgcgt ttgccgcgat cgaagatgca atgtctacga tattcggtgt acggggccga
       61 aactactttg tcagtcgttt agtcgaaatg ggtatggtcg ttctcatcgg ctgcctcttt
      121 ctgctttcga gcctgatcag cgggattatt cactggctac aggaaaatca tatcactttt
      181 tttcaaattg atttttcaaa cctgccgtac gtttgggata ttattacaaa cgccttgccg
      241 tatgtgctca tcgtactcac gttttatgtc acgtacaaaa ttttacccaa aacggaaatc
      301 tatgatcgtg cggcgatgct cggtgcgctg gtagcgagcg tgctgctgga attgacgctt
      361 tacggtttta gttattatgt gcaaaatttt gcaagatata atgtgtttta cggatccgtt
      421 gccggtgtgg tgatttctgt ttttacgatt tatttggctt ccattatttt acttgtcggt
      481 gcggaaataa ccgaaattgc caatacgcgt ttggagaacg agcgcgaatt ggcgcagacc
      541 ataagggagt ttgagtcatg acttcatcct ccctacccgt acttcatccg cgttacgatc
      601 tgattgaaaa aatcggtgaa ggcggggtgg gtatcgtcta tagagcgtgg gataaaatcg
      661 atcgctcgac ggtggttatc aaacaggcgc cggcgtcaac gattaatgat accttgcggg
      721 atgcgtattg gcgtgaattt gaatggatgt ttcgtgcaca tcatcccggc atgatgaagg
      781 cgctggcttg ggagaaatcc gaggacggag gtttcttctt tacgatgcaa ctggcagccg
      841 ggatcgatct gtttcaaaaa ataaaagatc gcggattttg gactaaaaaa agattcgata
      901 gttttatccg acaaattgtt tccgctttgt cctatgtgca tgcgcacggc atggtgcacg
      961 gtgacatcaa gccggagaac atcctgatcg aatcggccgg cgacgattgc aaggttacat
     1021 tgatagactt tggattcatt cacgcctggt ccaatcctcc tgtttcacgt gaccatcaag
     1081 gtacgctggc ttatacggcg ccggagatac tcaaaaagga gccgtacgat caacgcagcg
     1141 atgcgtatgc tctgggcgtg gtgttgtatg aactggccac aggcatcaac ccatttgcgg
     1201 atgaaaacgt tgttaatatc gtcgtgcatc atctgcaaaa aaacattacg cacgttgact
     1261 cggatttcga atggtggtcg caggattacg aaaaaatcat cgtttccctc ttgtctaaag
     1321 acccgcgcca acgttatcct cgtttggaag atgcatttaa gaaattgacg gatcaaaatg
     1381 cagtattgcc tccgttgttg tgccaccgtt ttgccgaacc gcggtcggta tggccggata
     1441 tttcagcgca gctccaaacg gatcatccta catccgttgt tcttcgtgcc ggtgagggac
     1501 tgggcgcgac cactatgctg cgcgaacttt atgtacgagc gatacattca ggatattctg
     1561 cacagtatat acgcctggat caagcagcgt cgctgtggga ggtactttcc ggttttgttc
     1621 ctgcatcggt tttggagacc ctgagcgaca cggatcgtgc aatcgaagaa attttgaaaa
     1681 ctatagcgcc taatgaaatt tatattttgg atcaagtgcg tttggatgta cccggtgtga
     1741 tggattttct ttcgcgattc acctacacgc tttcctcgaa ttcgggttct gtgattctat
     1801 caacgaccga acttcaaact gaaatttctt ttcctgcaaa aacatatgta ttgaaaaact
     1861 tagaacccca agatgtaaaa catttcttaa gcacgtggct ttttgacgaa gacgttgatg
     1921 aaaagcttgt ttcgcgcatg acgacgtgga caaacggaca tcccggctta ttatacgaag
     1981 ctacacgtga actgatggaa aaaagacaac tggtttttga agaacgtagc tggcggttga
     2041 cgcccacagc ggaatggaag ccatcttccg attggtcgga aaaatgggaa gccaaaatca
     2101 aagcgttaac tgacgacgaa attcgtctta tccgatggat ggctgtcgca gacaatgaaa
     2161 ttcctgtttt aactttgacc gcacttatgg aaaacgcagg tgccgagcaa aatataccgc
     2221 ggttggaatt attcggttgg attcgccgta aagagaatgc tgttaaaatt gttgctccgg
     2281 gccttcgtgc gtttctgatt caaaaagaaa ccgaagcgga acgccgcgct atgcatgaca
     2341 aacttttatc gtggtatgaa aaaaatgcgg cggatgcggt accgcagttg gcctaccacg
     2401 catttgaagg gaataatcag gagaagtcgg ttccgtataa actgcaatgg gccgaacgtg
     2461 aagtgtcgcg ttggaattat gcggaagctt tgcgtcaata cgaaaat
//
LOCUS       DAOQGF010000234         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_27800_length_2438_cov_7.436844, whole genome shotgun sequence.
ACCESSION   DAOQGF010000234 DAOQGF010000000
VERSION     DAOQGF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27800_length_2438_cov_7.436844"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..418)
                     /locus_tag="PK937_16220"
     CDS             complement(65..418)
                     /locus_tag="PK937_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34170.1"
                     /translation="MNEARKQRGPMISCPKCGHQIKVGRSKGQRGYYFEVIVKAYAEA
                     MGEEDLDLVNNWLKIKFNPKFDNVGGDEIKYGGSIEKEPVHRIEELYEQMRAFAMNFY
                     GVYIPEPNEVPLAEL"
     gene            complement(411..680)
                     /locus_tag="PK937_16225"
     CDS             complement(411..680)
                     /locus_tag="PK937_16225"
                     /inference="COORDINATES: protein motif:HMM:NF028094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNH34171.1"
                     /translation="MKRHTKIYLDFFGYNISSFIPCEVCGERANDIHHIEARGMGGKN
                     DAADVIENIIALCRKHHIELGDKKQFEDELTDIHFEFMRVNGRYE"
     gene            complement(999..1442)
                     /locus_tag="PK937_16230"
     CDS             complement(999..1442)
                     /locus_tag="PK937_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34172.1"
                     /translation="MSKKYYKKQANHESHIRKALAEYLHLQYPNTFWWFDLTGEKMPI
                     GQAKKLSKLKCRKGIPDFFMAEKTSKYNGLFIELKRDDRYVEKIIDDENFGSGKLKEH
                     IEEQRKMIEGLNDRGYYATFAVGFEDAKAVIDRYLSCVVVNETSR"
     gene            complement(1435..1638)
                     /locus_tag="PK937_16235"
     CDS             complement(1435..1638)
                     /locus_tag="PK937_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34173.1"
                     /translation="MPNEQKIEISQEFFKKIITEKLIDIDAELEQVEMDCIAQRDQIN
                     IDRLRVARAIMLDLIDAMWQKNV"
     gene            complement(1657..2043)
                     /locus_tag="PK937_16240"
     CDS             complement(1657..2043)
                     /locus_tag="PK937_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34174.1"
                     /translation="MNWLEMIIGILRDYEYLRILIEEKCKVAAKINKRYYISDFLGFS
                     FRLASFDSLSVSTVSDSFSFPLYWLTKSNEELAEIVAAEKKLKEEEYKKVADKFMESI
                     RLNAEHNERELYKKLKEKYEPKPINI"
BASE COUNT          604 a          553 c          436 g          845 t
ORIGIN      
        1 tcgagcattg cccttttttg tatgttgaat tttgtagcca ttatccaata tattttttga
       61 ttcttcacaa ttcagccagc ggcacttcat tcggctccgg tatataaacg ccataaaagt
      121 tcatcgcaaa cgcacgcatc tgttcataca actcctctat cctatgcacc ggttctttct
      181 ctattgaccc gccatacttt atttcatccc ctccgacgtt gtcaaatttt gggttgaact
      241 taatctttag ccagttgttt acaaggtcaa ggtcttcttc acccatagct tcagcataag
      301 cctttactat tacctcaaag tagtaaccgc gctggccttt agacctgccg acttttattt
      361 ggtgaccgca tttaggacag ctaatcatag gaccgcgctg ttttcttgct tcattcatac
      421 ctcccattta cacgcataaa ttcaaagtgt atatccgtta attcatcctc gaattgcttc
      481 ttatcgccaa gctctatatg atgtttgcga cacaacgcta taatattttc tatcacatct
      541 gccgcatcat tcttaccgcc cattccacgc gcctctatgt ggtggatgtc attagcccgc
      601 tctccgcata cctcgcacgg tataaagctg cttatattgt agccaaaaaa gtcaaggtat
      661 attttagtgt ggcgtttcat aaattacttt taagttaata aataacattg acattgtcaa
      721 gtgttttttt ccttgacaac gcttaaataa cttgacaaac cgcttttgat tcactacatt
      781 gttaaaaaat ataaggagag agaatgaacg ctaaacaagt cgcttacaaa acaatctata
      841 gcataggcaa gttgttattg attttaggat tcgccagctt gctctcaaat atgtttgttg
      901 ctttggcgtt gacgactata ggcgtgtatg atgcgccgta ttggttaaac gtgtgggtat
      961 ttattacgcc agtgtgtttg atatgcgggt ttttaatatc agcggcttgt ttcgtttacg
     1021 acgacgcagg ataaatatct atcaatcacc gcctttgcgt cttcaaatcc gacggcaaaa
     1081 gtggcgtaat agcccctatc atttagccct tcgatcatct tgcgctgctc ttcgatgtgt
     1141 tcctttagtt tgcctgaccc gaaattttca tcgtctataa tcttttcgac atacctatca
     1201 tcgcgcttaa gttcgatgaa taacccattg tatttagacg ttttttcggc catgaagaaa
     1261 tccgggattc ctttacggca cttaagcttt gatagtttct tagcttgtcc tatcggcatc
     1321 ttttcgccgg tcaaatcaaa ccaccaaaac gtgtttgggt attgcaagtg cagatactct
     1381 gctaaagcct tgcgaatgtg cgattcgtga ttggcttgtt ttttatagta ctttttagac
     1441 atttttttgc cacatagcat ctattaaatc aagcattata gcgcgggcta cgcgcaatcg
     1501 gtctatattt atttgatcgc gctgagcaat gcaatccatc tcaacctgct caagctcggc
     1561 gtcaatgtct ataagttttt cggtaataat tttcttgaaa aactcttgcg atatttctat
     1621 tttttgttcg ttcggcatga ctactccctt tattttttaa atattgatcg gcttaggctc
     1681 gtatttttcc tttagctttt tgtaaagctc acgctcatta tgttcggcgt ttagccttat
     1741 tgattccata aacttatctg ccactttctt atactcttct tctttcaact tcttttcagc
     1801 cgcgactatt tcagcaagct cttcgtttga ttttgtcagc caataaagcg ggaaagaaaa
     1861 actatccgat acggtgctaa cagaaagcga atcaaaagat gctaagcgga aagaaaaacc
     1921 taaaaaatca cttatataat atcttttatt tatttttgct gcgaccttgc atttctcttc
     1981 aattaaaatc cgcaaatatt cgtaatctct taaaatccct attatcatct ctaaccaatt
     2041 cataacaatc tcctcttatt attagttata cacttgtgtt aattaacttc tcggatggtg
     2101 gggtttcaga gcatagttcg ctccttaagt tttttacgct tttgaatagt tttgggaaaa
     2161 agaacttgca aagggttcgt tatgttttag gagcacttct cggcatggag ccggtcgtcg
     2221 tcgctttcgt gtggtaattg taggcggagg tctgttcccg ccgtagtctg atcgcgtgcg
     2281 atggactatc ccatgcccgc caatcaatgc caattacccg ctccgctaat tgcttagcca
     2341 aaccagtcgg gcgtttcagc cctcatttct tcctactttt taaccgccgg tgcgtacctg
     2401 tcatcaggga ttcacggggc ggcctttgca atatgtta
//
LOCUS       DAOQGF010000235         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_28491_length_2403_cov_6.086031, whole genome shotgun sequence.
ACCESSION   DAOQGF010000235 DAOQGF010000000
VERSION     DAOQGF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28491_length_2403_cov_6.086031"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PK937_16245"
     CDS             complement(<1..731)
                     /locus_tag="PK937_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34175.1"
                     /translation="MAMQDQQQQQIALNQLMELVQAPDFPGKLVPEHVTQFRSRLERF
                     SETEISAVTERIQEEIEKKRSSNKTLLVSKLEDVAALTKMELVLAEHKVPSHMINLLL
                     TLLVNKSSNFGTPIFDGKMEEFINDSIENAVLNIVLEKAASERTKGDKSGKGKELKEL
                     FEKKIRDTETLTHLIHIIDNVDLNNDATMKCVFVVNLVAEAMKREETMRKSESITMKD
                     MINNYLLSKLQMRLAEYNYPNDTLEG"
     gene            938..1534
                     /locus_tag="PK937_16250"
     CDS             938..1534
                     /locus_tag="PK937_16250"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNH34176.1"
                     /translation="MSVIFSEKELEKRKALIKVTYKLIAEHGFTNITLQDVADRADVS
                     KGIILYYFENKEALFEAVLEWLVKRIELHIRAEVSRAEDPISKIKAFNDATFLGIKEN
                     REFYLVYLDFLAQSSHTERLRNVNLAFYNQCSQVQSQILRDGIEKGIFREVNTSEYCI
                     ISRALIDGLCIRWLFDTPERFTFYKQTALQITLDYLKK"
     gene            1586..>2403
                     /locus_tag="PK937_16255"
     CDS             1586..>2403
                     /locus_tag="PK937_16255"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNH34177.1"
                     /translation="MTVIITTLGVIILIIIAMLLLTRYMAHKFLTVKLRPKTKTPADF
                     QLPYESVQFESEGRLLHGWLIRNNPQMPLVILVHGWESNAQGMLPHAQYLTPAGFNLF
                     LYDARGHGDSQPEDYMSLVRFTEDLENAYCFVRKKTEFNNHPVMLLGHSMGGATACLF
                     TARHPEIIAVVISSAFARFDLMIRDMFKTHTMLFKLLYPVLVSHWERMLKVKMENWHP
                     ADHLARVQCPVFVAFGGQDEMFTAKHFETFTGVATKERDEKLWLPEGHHRNLYE"
BASE COUNT          700 a          485 c          447 g          771 t
ORIGIN      
        1 ccttccagtg tatcgttagg gtaattgtat tccgctaaac gcatctgcaa tttggataaa
       61 agataattat tgatcatatc cttcatcgtt atggattcgc ttttgcgcat ggtttcttcg
      121 cgtttcatcg cttcagctac cagattgacc acaaaaacgc acttcatcgt tgcgtcgtta
      181 ttgaggtcaa cattatcaat tatatgaatc aaatgcgtta gcgtctctgt gtcacgaatt
      241 tttttctcaa aaagttcttt aagttctttt ccctttccac ttttatcgcc tttggttcgt
      301 tcggaagccg ctttttccaa tacgatattg agtacggcat tttcgatgga atcgttgata
      361 aattcctcca tcttaccgtc aaaaatcgga gtgccgaaat tagaagattt attgactaaa
      421 agtgtcaaca acaaattgat catatgtgaa ggcactttat gttcggcaag aaccagctcc
      481 atcttcgtta aagccgcaac atcttcgagc ttgctgacca ataaagtttt attagatgat
      541 cgcttttttt caatttcctc ttgtattcgc tcggtcaccg cactgatttc tgtttccgaa
      601 aagcgttcaa gacgcgaacg aaattgcgtc acatgttcag gaaccagttt gcccggaaaa
      661 tccggagctt gaacaagttc catcaactgg ttcagtgcaa tttgttgttg ttgctggtct
      721 tgcattgcca taggttattc catggtatcg gtgattttaa tcatttaaaa agttaacaca
      781 atcccgaata catagcaagc aaattagcta taagcctgac attacagacg atctctgcat
      841 cctatgaaca ggctcccaaa tttcaaatta attccttgtg tttaaattaa ttcatcatat
      901 atttgaccaa tcagtcaatt ttatagatat caacaatatg tccgtaatat tcagtgaaaa
      961 agaactcgaa aagcgtaagg ccctaattaa ggtaacgtat aagttgattg ctgaacacgg
     1021 ttttacaaat attacacttc aagatgtagc cgatcgtgct gatgtgagta aaggcataat
     1081 tttgtactat tttgaaaata aagaagctct atttgaagct gtacttgaat ggttggtcaa
     1141 aaggatcgaa ttgcatatac gtgccgaagt ttcacgtgcc gaagatccta tttctaaaat
     1201 taaagcattt aacgatgcta cttttttagg aattaaagaa aatcgagaat tttacttggt
     1261 gtatctggat tttctagcac aaagcagcca taccgagcgt cttcgtaatg tcaaccttgc
     1321 tttttataat caatgctcac aagtacaatc tcaaatttta cgcgatggta ttgaaaaagg
     1381 aatcttccgc gaagtaaata ccagcgaata ttgcatcata tcacgcgctt tgattgatgg
     1441 cctttgtatt cgatggttat ttgatactcc ggagcgattt actttttata aacaaactgc
     1501 acttcaaata acactcgatt atcttaagaa ataatttttt actaaaaaac tgaccgtcag
     1561 gtcaaatact aaaaacggag attgtatgac tgtcatcata acaactttag gagttattat
     1621 tttaattata attgctatgt tgcttctcac acgttacatg gctcacaaat tcctgacagt
     1681 caaactacgc ccaaaaacca aaacgccggc ggattttcaa ctgccgtatg aatccgtgca
     1741 atttgaaagc gaagggcgtt tactccacgg ttggttaatt cgtaacaatc ctcaaatgcc
     1801 attggttatt ttagttcatg gctgggaatc caatgctcag ggcatgcttc cccacgcaca
     1861 atatttaacg ccagccggtt ttaacttgtt tctatacgac gcacgcgggc atggtgattc
     1921 gcagcccgaa gattacatgt cgcttgttcg atttaccgaa gacctggaaa acgcctattg
     1981 ttttgttaga aaaaaaacag aatttaataa tcatccagtg atgctactcg gacattcgat
     2041 gggtggtgca acggcatgcc tgtttacagc gcgacatcct gaaataatcg ctgttgtcat
     2101 tagctcagca tttgcgcgtt ttgatctcat gattcgggat atgtttaaaa ctcataccat
     2161 gctttttaaa ttactttacc ctgttttggt ttctcattgg gaacgtatgc ttaaagttaa
     2221 aatggaaaat tggcaccctg ccgatcattt ggcgcgcgtc caatgccctg tatttgtagc
     2281 tttcggagga caagatgaaa tgtttaccgc taaacacttt gaaaccttta ccggcgtcgc
     2341 tactaaagaa cgtgatgaaa aactttggtt gcccgagggg catcatcgca atttatatga
     2401 att
//
LOCUS       DAOQGF010000236         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_28685_length_2393_cov_6.048332, whole genome shotgun sequence.
ACCESSION   DAOQGF010000236 DAOQGF010000000
VERSION     DAOQGF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28685_length_2393_cov_6.048332"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="ribF"
                     /locus_tag="PK937_16260"
     CDS             <1..688
                     /gene="ribF"
                     /locus_tag="PK937_16260"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNH34178.1"
                     /translation="AKLGVDALLIVKFDRSLMEKTGQEFVDQFLIQRAGMKKLLLGYD
                     HAFGKNRSGTFESLQPMAVEMNFIIEKLPAFDFDRIVVNSTMIRNMLKDGDVSTANDL
                     LGRRYIVEGTVQYGAGRGRTIGFPTANIQPTHMQKLIPMPGVYACRVNVDGSSFPAVT
                     NIGQRPTFDTSLQTVIEAHILDFDRDLYDRSVGLEFIYRIRDERKFSSREELIWQIRQ
                     DIRHAREQGF"
     gene            805..1074
                     /gene="rpsO"
                     /locus_tag="PK937_16265"
     CDS             805..1074
                     /gene="rpsO"
                     /locus_tag="PK937_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNH34179.1"
                     /translation="MAVTKEQKIEVIKKHGGTATNTGATEVQIALLTQHINELTKHLQ
                     EHKKDHSSRRSLLKLVGQRKRLLTYLTKKDIEKYRSLIAELGIRK"
     gene            1333..>2393
                     /locus_tag="PK937_16270"
     CDS             1333..>2393
                     /locus_tag="PK937_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNH34180.1"
                     /translation="MFTIKSRSMELGGRTLTIEHGRVARQADGAVWITYGDTTILATV
                     VCAKEMKEGIDFFPLMVDYRESSYSAGKIPGGFFRREGRPSEKEILSSRLIDRPLRPL
                     FPEGFFYDTIINVNVISSDQEGSADVIGGIGASAALMVSDVPFDNPIASVRVGRIDGQ
                     MIINPTFKQLETSDMDIVVSGTKDSIAMVEGESREISEEDMLNAILFAHDAIKQVIAL
                     QEELAKDIAPVKRTFDAATLPQEFINDITAIAKPVVYEADRIADKSLRNEKLKELKKS
                     LCEQFAEKYPEMDAAIKKMVDDLQYADMRSMILDEKRRLDGRTTCDIRQIVCQTSVLP
                     RVHGSALFTRGQTQALVATT"
BASE COUNT          660 a          526 c          568 g          639 t
ORIGIN      
        1 cgccaagctg ggggtggatg cgcttcttat tgtaaaattt gaccgttcct tgatggaaaa
       61 aacagggcaa gaatttgttg accaattttt gattcaacga gccggcatga aaaaattact
      121 gttaggatat gaccatgctt ttggaaaaaa tcgtagcggc acattcgagt cgttacagcc
      181 gatggctgtc gaaatgaatt ttattatcga aaaattgccg gcgtttgatt ttgatcgtat
      241 cgttgttaat agcacgatga ttcgcaatat gttgaaagac ggagatgtat ctacggccaa
      301 tgacctttta ggtcgtcgtt atatcgtcga aggcacggta cagtatggcg ccgggcgagg
      361 tcgcacgatc gggtttccta cggcaaacat ccaacccaca catatgcaga aactcatccc
      421 gatgccgggc gtttacgcat gccgtgtgaa tgtggacggt tcctcatttc ctgcggttac
      481 taatattggc cagagaccta cgttcgacac aagtttgcaa acagtgatcg aagcgcatat
      541 ccttgatttt gatcgtgatt tgtatgatcg atccgtcggc cttgagttta tttatcgtat
      601 tcgggacgaa cgtaaatttt catcccgcga agaattgatc tggcaaattc gtcaagatat
      661 tcgtcatgca cgcgagcaag gcttttaggt tttttttatt aaaaaaacaa ttgacttaca
      721 tatttgcatt atatacattc acgcgctttt gggttaaaag taagcccaat aatttcaaaa
      781 acataaagga gtaaagagaa tcacatggcc gttaccaaag aacaaaaaat cgaagtgatt
      841 aaaaagcacg gcggaactgc gacaaatacc ggggccacgg aagtccagat cgcgctgctc
      901 acgcaacaca tcaacgagct cacaaagcac ctgcaagagc acaaaaaaga tcacagctcg
      961 cgccgttcgt tgctcaaact cgttggtcag cgcaaacgtt tactcacgta tttgaccaag
     1021 aaagatattg agaaatatcg ttcattgatc gcagaactcg gtatccgtaa gtaatttgta
     1081 tcaggctgtc ccaaaagcta tccgttttgt tctctttttg ttaggttgaa cacggtgcgt
     1141 ttttgggaca gcctgttttt gtttatatgt tcattgaaat ggtgggattc gtctgttatc
     1201 tgcatcaacg tcattctaaa cgaaacctga cgcacagttt tcgcttaggc tgacgcgacg
     1261 tgcggattgg caaacaaaac gcatcacacc gtttcttttt atagtaatac ctcataatta
     1321 aggagaaatg gtatgtttac aatcaaatcc cgatccatgg aactgggcgg gcgtacgctc
     1381 acgatcgaac atggtcgtgt agcgcgtcaa gccgatggtg ccgtgtggat tacctatggt
     1441 gataccacga ttttggcaac cgtcgtgtgt gccaaagaaa tgaaagaagg cattgatttt
     1501 tttccgctga tggtggatta ccgcgagagt tcgtattctg ccggtaaaat acccggcggt
     1561 tttttccgtc gtgaaggccg tccctcggaa aaagaaatcc tctcaagtcg ccttattgat
     1621 cgtccgcttc gtccgttatt tcccgaagga tttttttacg atacgatcat caatgtcaat
     1681 gtgatttcgt cggatcagga aggcagcgca gatgtgatcg gcggtattgg cgcttcggcc
     1741 gcacttatgg tcagtgatgt tccgtttgat aatccgatag cctccgtgcg tgtagggcga
     1801 attgacggtc aaatgatcat caatcccaca ttcaaacaat tagaaaccag cgatatggat
     1861 atcgtggttt ccggcaccaa agactccatc gccatggtgg agggggagtc ccgcgagatt
     1921 tccgaagaag atatgcttaa tgcgattctt tttgcgcatg atgctatcaa acaagtcatt
     1981 gcgcttcagg aagaattagc caaagatatt gcgcccgtaa aaagaacatt cgacgccgca
     2041 acgttaccgc aggaatttat caacgacatt accgcgatcg ctaagccggt cgtgtatgaa
     2101 gccgatcgca tcgcagataa atcgctgcgc aatgagaaac taaaagaact gaagaagtcg
     2161 ctttgcgaac agtttgcaga aaaatatccg gagatggatg cggcaatcaa aaaaatggtt
     2221 gatgatcttc agtatgccga tatgcgttcg atgatcctgg atgaaaagcg gcgcttagac
     2281 ggtcgtacga cgtgcgatat tcgccagatc gtatgccaaa ccagcgtact tcctcgcgtt
     2341 cacggatcgg cactttttac ccgcggccaa acccaagcgc ttgtagccac cac
//
LOCUS       DAOQGF010000237         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_29590_length_2349_cov_5.304272, whole genome shotgun sequence.
ACCESSION   DAOQGF010000237 DAOQGF010000000
VERSION     DAOQGF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29590_length_2349_cov_5.304272"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PK937_16275"
     CDS             complement(<1..1530)
                     /locus_tag="PK937_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNH34181.1"
                     /translation="MIFSSFKKRLESLTVRYFADSYISTKIGYILVPIFAWAYAAMIP
                     FSERAISRETLIVALTISFLVWVMLLALIQRRLIRHARHAVHRFLGGQSVSFDDAQGA
                     VHELLRFPGRNTWTELVLFILVAIVPFTLIFPQSTAMLSGMGIGTMYAALFNYILTER
                     WAAALILFIREHRKNLQIADTASHRMSRLYVTVASVSVISVGISVWFIIDAYNEYLRS
                     FTIDHFPTTAAMHAFLSQQTVQLQHSLLIAVGMMTACTFGIVIILSVSLDGVLRDLSK
                     EIRKLEVGDFSGSKKYRTPHGMGFLLIQLDSAVHKIRDRINYLQRNGVDMYTTTNKIA
                     AISSDQNQSLVRQSSSLSETSSTLEEMVRSSNQIADHASAVVGQAEDTERSAKKGVLR
                     MEETVRMIKNVESKNQLSLTEVIQLSESIQEIEQILRFINSVADETNLIALNATIEAS
                     SAGEHGKRFKVVANEVRSLSDRVTSSVKNIQRMIDQVQEATTRLVTVAQETSEHTVSS
                     AD"
     gene            complement(1676..1978)
                     /locus_tag="PK937_16280"
     CDS             complement(1676..1978)
                     /locus_tag="PK937_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNH34182.1"
                     /translation="MNSNKLYVGNLSYRADDDGLTQLFSQAGSVTSAKIIVDKLSGKS
                     KGFGFVEMATAEEAEKAKEMFHNYSFMERPLIVNEAKPMEKKPFGGRDRGGFRKDQ"
BASE COUNT          684 a          497 c          569 g          599 t
ORIGIN      
        1 atcggcgctg ctgaccgtat gttcggaagt ttcttgcgcg acggtgacta agcgcgttgt
       61 ggcctcctgt acctgatcaa tcatacgttg aatattttta acggagcttg taacgcgatc
      121 cgaaagcgag cgtacttcat tggcaacgac tttgaatcgt ttgccgtgtt cgccggcgga
      181 actagcttcg atcgtggcgt taagtgcaat gagattggtc tcgtcggcta ccgagttaat
      241 gaaacgtaaa atttgctcta tttcctgaat actttcgctg agttggatga cttcagtgag
      301 gctgagttga tttttggatt ccacattttt gatcatgcgc acggtttctt ccatgcgaag
      361 aacgcctttt ttagccgatc gctcggtatc ttcggcctga ccgactacgg cgctcgcatg
      421 atcggcgatc tgattggatg agcgaaccat ttcttccaat gtcgaggagg tttctgacag
      481 tgaagaagat tggcgtacca acgattgatt ttgatcggaa gagatcgcgg ctattttatt
      541 ggtcgtcgta tacatatcca caccgttacg ttgcagataa ttaatacggt cccggatttt
      601 atgcacggcg gagtccaact ggatcagtag aaagcccata ccgtgcggtg tgcggtattt
      661 tttggagccg gaaaaatcgc caacctcaag tttgcgaatc tctttcgata agtcacggag
      721 tacaccgtcc aatgaaaccg ataaaatgat aacgatacca aaagtgcagg ccgtcatcat
      781 acccaccgcg atgagaagcg agtgctgaag ttgaacggtc tgttgtgaaa ggaaagcatg
      841 cattgccgcc gttgtcggga aatggtctat tgtaaatgaa cggaggtatt cattgtatgc
      901 gtcgattata aaccacacgc tgatgccaac gctgatgact gaaactgaag cgaccgtcac
      961 atataaccga ctcatacggt gcgacgccgt gtcggcaatc tgaagatttt tgcggtgttc
     1021 acggataaaa agaatcaagg ccgctgccca gcgttcggta aggatgtagt tgaatagcgc
     1081 cgcgtacatc gtaccgatac ccattccgga gagcatggct gttgactgag ggaaaattag
     1141 agtgaaggga acgatggcaa ctaaaatgaa caggactaat tcggtccatg tgttgcgacc
     1201 gggaaaacgt aaaagttcgt gtacggcacc ctgcgcgtcg tcgaaactca cactctgtcc
     1261 gcctaagaag cgatgcacgg cgtggcgggc gtgccgaatg agtcgtcgtt gtatgagcgc
     1321 cagcaacatg acccaaacaa gaaaagaaat agtaagagca acaatcaatg tttcccgcga
     1381 aatcgcgcgt tccgaaaaag gaatcattgc ggcgtaggcc cacgcaaaaa tcggtacgag
     1441 gatatagccg atctttgtgg atatgtagga atccgcaaaa taacggactg tcagcgattc
     1501 taaacgtttt ttaaaagagg agaaaatcat attgatgaat attgacccga ttatcgtacc
     1561 gattgtaata ctttttgatg tggctttcaa caagaaagga gaacggagga gtgtaaaaaa
     1621 aaaggctgtt ccaaagttgg aacagcctgt atctttaatc gagatatttg ctcaattatt
     1681 gatctttacg aaaaccgccg cgatcacggc cgccgaaggg ctttttttcc atcggtttgg
     1741 cttcgttgac gatcagagga cgttccataa aggaatagtt atggaacatt tctttggctt
     1801 tttcagcttc ttcagccgtt gccatttcga caaaaccaaa gcctttggac ttccccgaca
     1861 acttgtctac aatgattttg gccgaggtga ccgagccagc ttgcgaaaag agctgggtaa
     1921 gaccatcgtc atcagcgcgg taggacaggt tgcccacgta cagtttgttt gagttcatgc
     1981 gaacatctcc ttcagaaaaa aaaatcatgg gttaaataca aaaaaacctc aaagcgccca
     2041 ttcactttga ggttgtaaaa ctttatacac acgaaatggc tttaagacgg gaataagtca
     2101 caaagacaag accggaaaaa gaatacttgc tcaatcgagc atcgaaactt ttaaaacaat
     2161 taccaaataa caaaaagcat tcacactgct tacaattcaa atatgaccaa tgcataaaac
     2221 gaagtcaaga aaataattaa gtaatttttc aaacggcaca gaagggaaat cggagctagt
     2281 tttagaccgt ttgatgcggc atggagtaaa aacaaatttg acattcgaga gggaatcagc
     2341 tatattttt
//
LOCUS       DAOQGF010000238         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_29841_length_2336_cov_7.581324, whole genome shotgun sequence.
ACCESSION   DAOQGF010000238 DAOQGF010000000
VERSION     DAOQGF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29841_length_2336_cov_7.581324"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PK937_16285"
     CDS             complement(<1..1131)
                     /locus_tag="PK937_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34183.1"
                     /translation="MMHNKNNYTLYAGLTSTTTAAACFDIIASALTETASFHDESGQN
                     IFGRPLLHVSARNLGQAMGLASSGIRASATVTLQALLQDTEMMHACVRRHLPVVLVAF
                     EREQNENGEGSFCDTRARRMAGEAGWIQLIAANHQELADMIIAAHRIAELTMIPVMVL
                     ADMTRSSAGKVTFPNESALRTYLGDPDDMIETPTSGQKIIFGKIRRRIANWFHSDYPI
                     MLGADKSGLMHAFEAAAGGVYFSEAVAPIIDAAWDEFQTQFDRSYKSVETLGSEKAEQ
                     LILSGGAVIAALRSGAEQLKTAKIKAGVVNIRVWQPFPGAAVCEAISGKKNVAVLEPL
                     SGGFIQEGPLYRETISALQKAGENAGQKKSPPYPEYPSINERE"
     gene            complement(1128..2000)
                     /locus_tag="PK937_16290"
     CDS             complement(1128..2000)
                     /locus_tag="PK937_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNH34184.1"
                     /translation="MFSEIIIAVTIMVGLGLLFASVLAFAYKKLRVEEDPRIDQVESM
                     LPGSNCGACGRPGCRAFAESVVGGINKPGKCTVSSPSAVASIAEYLGVAAGFEEKRVA
                     RLLCAGGKQESHNQADYYGKRTCRSVAMVTGGTKDCVWGCLGLGDCESVCDFNAITMN
                     SDGLPVVDPVQCTACNDCVDVCPKKLFTIMPVSQKLIVQCRSLLEGDAALERCSVACN
                     ACTRCVADAAPGLIQMKNNLPVIDYSQNVLARPDATRRCPTRAIVWVEGRQFEKAPAT
                     PLPIGRVEVLPEEL"
     gene            complement(2030..>2336)
                     /locus_tag="PK937_16295"
     CDS             complement(2030..>2336)
                     /locus_tag="PK937_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNH34185.1"
                     /translation="ATVSDTPTVSAPVEEEPVISNEAWLETIRCTSCNECINLNPNIF
                     KYNSAKQAYIGDARAGSYLHIVQAAEKCPAKCIHPGLPLNPSEDGLDALIQRAAVFN"
BASE COUNT          548 a          629 c          565 g          594 t
ORIGIN      
        1 ttcacgttca ttgattgacg gatattccgg gtacggcgga gatttttttt gcccggcatt
       61 ttcgccggct ttttgtaatg ccgaaatcgt ttcgcgatat aacggtcctt cctgaataaa
      121 tcctccggac agaggttcaa gcacggccac atttttttta ccgctgatgg cttcgcaaac
      181 ggcggcaccg ggaaagggct gccacacgcg gatattcact acaccggctt ttattttggc
      241 ggttttgagt tgttctgcac cggaccgaag tgcggcaatg acagcgcctc cggagaggat
      301 gagctgttcg gctttttctg aacctaaagt ttcgacgctt ttatatgagc gatcaaactg
      361 tgtttgaaat tcgtcccacg cggcatcgat gatcggtgcg accgcttccg agaaatacac
      421 gccgccggcc gccgcttcaa acgcatgcat caggccggat ttatcggcgc ccaacataat
      481 cggatagtcg ctatgaaacc agttggcgat acgccgccgt atttttccga aaattatttt
      541 ttgtccggat gtcggcgttt cgatcatatc atcgggatcg ccaagataag tgcgtaatgc
      601 gctttcattt ggaaaagtaa ctttgcccgc cgatgatctt gtcatatcgg ctaaaaccat
      661 taccggaatc atcgtcagtt cggctatacg atgcgccgct atgatcatat cggccaattc
      721 ctgatggttt gccgctatca attgtatcca tccggcttca ccggccatac gccgtgcacg
      781 tgtatcgcaa aacgatcctt cgccgttttc attttgttca cgttcaaatg ccacgagtac
      841 tacgggcaaa tgacgacgaa cgcaggcgtg catcatttct gtatcttgaa ggagcgcctg
      901 aagtgtaact gttgcagagg cgcgaatacc cgaagaagcc aatcccatcg cctgcccaag
      961 attgcgggcc gatacgtgca gtagcggtct tccgaaaata ttttgcccgc tttcgtcgtg
     1021 aaaggaagcc gtttccgtca aagccgatgc gatgatatca aaacacgctg ccgccgtcgt
     1081 ggtgctggtc aaaccggcgt aaagagtata attgttctta ttgtgcatca tagttcctca
     1141 ggtaaaactt ccacacgccc tatcggtagc ggcgttgcag gcgctttttc aaattgacga
     1201 ccttccaccc acacaatggc tcgtgtcggg caacggcgcg tcgcgtcggg gcgcgctaat
     1261 acattttgac tataatcaat cacgggcaaa ttattcttca tctggatcaa acccggcgct
     1321 gcatcggcca cacaccgggt gcacgcattg caagctaccg aacaccgttc caatgcggca
     1381 tcgccctcaa gcagtgaacg gcactgtacg atcagtttct gactgacggg catgatggta
     1441 aaaagttttt tggggcatac atccacacaa tcgttgcacg ccgtgcactg gacgggatcg
     1501 acgaccggta gaccgtcgct attcatcgtg atcgcgttaa aatcgcagac cgattcgcaa
     1561 tctccgaggc ccaaacatcc ccatacacaa tctttcgttc cgccggtaac catcgctaca
     1621 ctgcggcagg tgcgttttcc ataatagtcg gcctggttgt ggctttcttg tttaccgccg
     1681 gcacataaaa gacgtgctac gcgtttttct tcaaagccgg cggccacacc gaggtattcc
     1741 gcgattgatg ctaccgccga aggtgaactg acggtacact tgcccggttt attgatgccg
     1801 ccgacgacac tttctgcaaa tgcacggcaa cccgggcgac cgcatgcgcc acagttggaa
     1861 cccggtaaca tagattcgac ctgatcaatg cgcggatctt cttcgacacg cagcttttta
     1921 tacgcgaacg ctagtaccga tgcgaaaagc agcccgagac cgaccatgat cgttacagct
     1981 atgatgattt cggaaaacac gataaattct tcttacgtaa caaaagtttt tagttaaaaa
     2041 cggcagcccg ctggatcaac gcatccaatc cgtcttccga aggattgagc ggtaatccgg
     2101 ggtgaataca tttagccgga catttctcgg ccgcttgcac gatatgcaaa taacttccgg
     2161 cacgggcgtc gccgatatac gcttgtttag cgctgttata cttaaaaata ttcggattca
     2221 ggttgatgca ttcattacaa cttgtacaac gtatcgtttc aagccaagct tcgttggata
     2281 tgaccggttc ttcctccacc ggtgcagata ccgtcggtgt atcggacacc gtagcc
//
LOCUS       DAOQGF010000239         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_30223_length_2317_cov_4.323165, whole genome shotgun sequence.
ACCESSION   DAOQGF010000239 DAOQGF010000000
VERSION     DAOQGF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30223_length_2317_cov_4.323165"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK937_16300"
     CDS             complement(<1..293)
                     /locus_tag="PK937_16300"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH34186.1"
                     /translation="MIPTLFEIPIFGGIPIHSFGLMMAAAFLTSSFVLRRLFKNAGYS
                     EELSEKIILVAAIAGLAGSKLYYLLFEAFERFKKYPMEMLFSGAGLTWYGGFF"
     gene            complement(354..833)
                     /locus_tag="PK937_16305"
     CDS             complement(354..833)
                     /locus_tag="PK937_16305"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNH34187.1"
                     /translation="MQAQRSLPLSSWHIVFPNDTNPHGTMFGGKVMAIMDIQAGIVAT
                     QYCHRMVVTASTEAVDFRNPVKVGDRLETIARVVYVGKTSMVVRIDAYAENPLSGKRK
                     HCTTAYFNMVALDEDGAPTTIPPLIVETDEEKRDFELAKHIKEAATERKKRFADTSL"
     gene            complement(848..>2317)
                     /locus_tag="PK937_16310"
     CDS             complement(848..>2317)
                     /locus_tag="PK937_16310"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNH34188.1"
                     /translation="IKNIPPELAVNIKIDRPEIYFGELMNESDYVIVKTSKEEFDYPL
                     GESNQHTMYKEESGVSIGSFARKMLFAIRFNKFNLLLNDYIQAQSKVLYYRNIRDRVE
                     KIAPYIRWDRDPYLVAENGRLYWMMDGYTITDQYPYSAMSEERVGSGAFARKRYFNYI
                     RNSVKVVIDAYNGKTDLYAFQPDSDPLIRVYGKMFKGVFKEYATMPDELKKHVRYPQD
                     LFDIQASHFASYHVSDANVFFNKEDVWNVAQEKYGDDVQTMESYYMIMRLPGETKEEF
                     ITLLPFTPNNKSNMIAWFCVRSDGDNYGKLLVYRFPKTELVYGPMQVESRIDQTPEIS
                     EKLTLWNQQGSRVTRGNMLVVPINNSLLYVEPLYLQSNQSRLPELKKVIVAYGNSIFM
                     EDNLEIGLEKIFGGNYGDFGTSSTVAPMSENKATTDNVSSGLSSDQSRALRELSRSAM
                     QNYNNAQDALKRGDWAKYGEFLERLKRDLDKLNEQSQKF"
BASE COUNT          624 a          569 c          484 g          640 t
ORIGIN      
        1 aaaaaaaaac cgccatacca ggtaagtccc gcgccgctaa acagcatctc catcggatat
       61 tttttaaaac gctcaaacgc ttcaaaaaga agataataca atttggaacc ggctaaaccg
      121 gcaatcgctg cgacaagaat gattttttcc gacaattctt cggaatatcc ggcatttttg
      181 aacaaccgcc gcaacacaaa actggaagta agaaaagccg ccgccatcat caacccaaaa
      241 ctgtgaatgg gaatgccgcc gaagataggt atttcaaata acgtaggaat catgggctat
      301 aactttttta tgtaaattat ttgttgatac taataaatac tatacgccac ccatcacaac
      361 gaggtatcag caaatcgttt cttacgttca gtcgccgcct ctttgatgtg tttggccagt
      421 tcaaaatcgc gtttttcctc atctgtttca acgatcaacg gcggtatcgt cgtcggcgca
      481 ccgtcttcat ccagcgcgac catattaaaa taggctgtcg tacaatgttt tcgttttccg
      541 gataacggat tttcggcata ggcgtcaata cgaacgacca tcgaagtttt accgacatat
      601 accacccgcg caattgtttc caaccggtct ccgactttga ccggattgcg aaaatccacg
      661 gcttcggtgg acgccgtcac caccatgcga tgacaatatt gtgtcgcaac aatgccggcc
      721 tgaatatcca tgatcgccat gaccttaccg ccaaacatcg ttccgtgcgg attggtatca
      781 ttgggaaata caatatgcca gctgctgagc ggcaatgaac gttgggcttg caaacggtta
      841 tccttcatta gaatttttga ctttgttcgt tgagtttatc caaatcacgc ttgagacgct
      901 caaggaattc gccatactta gcccaatcac cgcgtttgag cgcatcttgt gcgttattat
      961 aattttgcat cgccgagcgt gaaagttcac gcagagcccg cgattgatcc gacgacaagc
     1021 cgcttgatac gttatcggtc gttgctttat tctcagacat cggtgccacg gtactcgaag
     1081 ttccgaagtc gccgtaatta ccaccgaaaa ttttttccaa tccgatttcc agattgtctt
     1141 ccataaaaat actgttgccg taggccacga tgactttttt cagttccggc agacggcttt
     1201 gattggactg cagatacagc ggctcaacgt agagcagtga attattgatc ggaacgacga
     1261 gcatattgcc gcgcgtcacc cgtgaacctt gctgattcca cagcgtaagt ttttctgaaa
     1321 tctccggcgt ttgatctata cggctttcaa cctgcatagg accgtacacc agctccgttt
     1381 tgggaaaacg gtacaccaga agttttccgt aattgtcccc gtcactacgc acacaaaacc
     1441 aagcgatcat attggatttg ttattgggcg taaaaggcaa caacgtgata aactcttctt
     1501 tggtttcgcc cggaaggcgc atgatcatgt aataactttc catggtctgc acatcatcgc
     1561 cgtatttttc ctgagcgaca ttccacacat cttccttatt aaaaaacaca ttagcatccg
     1621 aaacgtgata cgatgcaaaa tgggaagcct gaatgtcaaa cagatcctgc ggataacgca
     1681 catgtttttt gagttcgtcc ggcatggtag cgtactcttt gaacacccct ttgaacattt
     1741 ttccgtaaac acggatcagc ggatcgctgt cgggttgaaa tgcatacaga tcggttttgc
     1801 cgttgtacgc atcaatcacg actttgacgg aattgcggat ataattgaaa tagcgtttgc
     1861 gtgcaaaagc cccggaaccc actctttctt ccgacatagc tgagtacggg tattgatccg
     1921 tgatcgtata tccatccatc atccagtaaa gacgtccgtt ctccgccacg aggtacggat
     1981 cgcgatccca ccggatatac ggcgcgattt tttcgacgcg atcgcggata ttacgatagt
     2041 ataatacttt actttgcgcc tgaatatagt cgttgagcag gagattaaat ttgttaaaac
     2101 gaatcgcaaa aagcatctta cgggcaaagc tgccgatact tacaccggat tcttccttgt
     2161 acatcgtatg ctgattggat tcgcccaaag gatagtcaaa ttcttctttc gaagttttga
     2221 cgatcacgta atccgattcg ttcatcagtt caccgaaata aatttccggg cgatctattt
     2281 tgatattcac cgctaattca ggcggaatat ttttgat
//
LOCUS       DAOQGF010000240         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_32812_length_2207_cov_6.026022, whole genome shotgun sequence.
ACCESSION   DAOQGF010000240 DAOQGF010000000
VERSION     DAOQGF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32812_length_2207_cov_6.026022"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="fabF"
                     /locus_tag="PK937_16315"
     CDS             complement(<1..952)
                     /gene="fabF"
                     /locus_tag="PK937_16315"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNH34189.1"
                     /translation="MSKRRVVITGMGAVTPIGNTVEEFWKGLLEGRNGVGPITLFDAK
                     DHVTKFAAEVKNFDPSLYTDKKEARRMDRFCQFAVAASKMAIEDAKLDVKNLDANRIG
                     CVIGSGIGGFQTFEEQFETYMAKGPGRVSPFFIPMMIIDIAPGQVAIQHGLKGPNYAT
                     VSACATGSHAIGDAFILIQRGDADAMVCGGAEAGICRMGVAGFNALKALSTRNDDPMH
                     ASRPFDKERDGFVIGEGAGIVVLEELEHALKRGAMIYGEMAGIGYTDDAYHITAPAEG
                     GEGAARAMAIACKDAGVKPEEVGYINAHGTSTEYNDKNESA"
     gene            complement(1132..1383)
                     /gene="acpP"
                     /locus_tag="PK937_16320"
     CDS             complement(1132..1383)
                     /gene="acpP"
                     /locus_tag="PK937_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNH34190.1"
                     /translation="MTREEITKKVTEIIVKKLGVTETEVKPEASYVEDLGADSLDQVE
                     LVMEFEDSFSLGDKIPEDEAAKLTTVGSTIDYLVNRLGK"
     gene            complement(1448..2200)
                     /locus_tag="PK937_16325"
     CDS             complement(1448..2200)
                     /locus_tag="PK937_16325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNH34191.1"
                     /translation="MCSLTNRIAVVTGSSRGIGKAIAVEFLKHGASVVINGKTSERVQ
                     QTTDELNALGFSGKFVSCVADVSVATEAEALIRKATEAFGRLDILVNNAGVFKNSLMV
                     RMDEQEWNWVIENNLKSAFFCSKYAAKIMMKQRYGRIIHMSSVVALGGNPFQANYVAS
                     KAGIDGLMMASAKELAPYGITVNSVAPGYIKTDMTKDFSESVVAEILKYIPLGRPGSA
                     EEVAHAVKFLASDEASYMTGQVIQVDGGRVIR"
BASE COUNT          537 a          619 c          453 g          598 t
ORIGIN      
        1 ctgcactttc gtttttatca ttatattcgg tggacgttcc gtgggcattg atgtatccga
       61 cttcttccgg cttcacaccc gcgtctttgc acgcaatcgc catagcacgt gcagcacctt
      121 cgccgccttc agccggtgcc gtgatatggt acgcatcgtc ggtatagcca ataccggcca
      181 tttctccgta gatcatagca ccgcgtttga gtgcgtgctc aagttcttcg agcaccacga
      241 tgccggcacc ttcaccgatc acaaatccgt cgcgttcttt gtcgaaagga cggctcgcgt
      301 gcatcggatc atcattgcgg gtggacagtg ctttgagggc attaaaccct gcaacaccca
      361 tacgacaaat acccgcctct gcaccaccgc acaccatcgc gtcggcatca ccgcgttgga
      421 tcaaaataaa tgcatcaccg atagcatgac tgcctgtagc acacgccgac accgtcgcgt
      481 aattcggccc cttgagaccg tgctgtatgg ctacctgacc cggcgcaata tctatgatca
      541 tcattggaat aaagaacggg ctgacacgtc cgggcccttt ggccatgtac gtttcaaact
      601 gttcctcaaa cgtctgaaat ccgccaatac cggaaccgat cacacaaccg atacgattgg
      661 catccaaatt ttttacatcc agcttggcat cttcaatcgc catttttgat gccgctacgg
      721 caaattggca aaagcgatcc atacggcgtg cttctttttt atccgtatag agcgacgggt
      781 caaaattctt tacttctgcc gcaaacttgg taacgtgatc tttcgcatca aatagtgtta
      841 tcggtccgac accgttgcgt ccttccagta atcctttcca gaattcttca accgtattgc
      901 cgatcggcgt cacagcgccc ataccggtga tcactactct gcgtttactc atagtcgctc
      961 cttacattgg cgatgaatgt taaaaaatta cctctcgtag cgtgataccg caaaaaaggc
     1021 caccgtacaa atcggcatga atcttaagcg gatacgatat aagtttgata aaaaaaggct
     1081 aatccgccga ataactgaat atccgacgca ttagcctatg ctataattcg cttatttacc
     1141 gagacgattt acaagataat caatcgtcga acccacggtc gtgagtttgg cggcttcatc
     1201 ttccgggatt ttgtcgccga gactgaaaga atcttcaaat tccatcacca gctctacttg
     1261 atccagcgag tccgcaccga gatcttctac atacgaagct tccggcttta cttcggtttc
     1321 cgtaacaccg agttttttaa cgataatctc ggttactttt ttcgtgattt cttcacgtgt
     1381 catggttttt ctcctttttt gaagagttgt tgtttaggta tatattctaa agttcactcc
     1441 aacatcatta ccggattaca cgcccgccgt ccacttggat gacctgaccg gtcatatagg
     1501 aagcttcatc cgacgctaaa aacttcactg catgggcgac ttcttctgcg ctgcccgggc
     1561 gaccaagcgg aatgtatttt aatatttccg cgacgaccga ttcggaaaaa tctttcgtca
     1621 tatcggtttt gatataaccc ggagcaacgg aattaaccgt tatgccgtac ggtgccaatt
     1681 cctttgcgct ggccatcatt aatccgtcaa tccccgcttt ggatgcaaca tagttggctt
     1741 gaaacggatt cccaccgagc gctaccaccg aagacatatg tatgatgcgc ccgtaccgct
     1801 gcttcatcat gatcttcgcc gcatatttgg aacagaaaaa agcgcttttc agattgtttt
     1861 cgatgaccca gttccattcc tgttcatcca tgcgcaccat caagctattc ttaaatactc
     1921 cggcattgtt aaccaaaata tccaaccgac caaatgcctc tgtcgccttg cgtatcagcg
     1981 cctccgcctc cgttgcaaca gatacatcgg ccacacagga tacaaacttt cccgaaaatc
     2041 ccaaggcatt caattcatcc gtcgtttgtt gaacacgctc ggaagttttt ccgttgatca
     2101 caaccgatgc gccgtgtttc aaaaactcta cggcgatcgc tttaccgatt ccgcgggagg
     2161 agccggtcac tacggcaatt cgattcgtca aagaacacat ggcgttc
//
LOCUS       DAOQGF010000241         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_32818_length_2207_cov_4.345260, whole genome shotgun sequence.
ACCESSION   DAOQGF010000241 DAOQGF010000000
VERSION     DAOQGF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32818_length_2207_cov_4.345260"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1277)
                     /locus_tag="PK937_16330"
     CDS             complement(150..1277)
                     /locus_tag="PK937_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNH34192.1"
                     /translation="MEDIKKIPFLTKELILSNKDSMVGSNYSKSKLLYYSTGGTTGIP
                     MSFFNEKGVSRSKEFAFMTSQWKRVGYQVNDRIVTLRGNVIPSAKNQVFWEYEPIKNR
                     LILSTYHMNENSLTTYIEKIRQFKPQFIHTYPSAVTVLAKFMVAHHIPPFPTLKAVLC
                     SSEAFYPGQRELLENAFQCRIFSWYGLGEMVALAGECEYSHKYHIFPEYGYVELIDAN
                     GKIIDSEGTLGEIVGTGFDRDIMPFIRYRTGDFAEYSDEKCSCGRNYRLLKRVEGRWL
                     QEQIFTKKKNLISVTALNMHTPVFENVAQYQFEQSEIGRLILKLVPKKTFQNSDIADI
                     KSTLTAKLDPSDIELEIKLVDTQQIESGRSGKFRFLIQNIK"
     gene            complement(1570..>2207)
                     /locus_tag="PK937_16335"
     CDS             complement(1570..>2207)
                     /locus_tag="PK937_16335"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNH34193.1"
                     /translation="HTMGTPDQGGDEFEKSSVGNSFQGWLVRIYKRALTFFGGTGGVG
                     AKLAIKLGYAIFGRTDNVIRSGNWYGNDTLWRMVLDLNKILFYYNASGKMLPSKRRYL
                     TVIDGIVAGENNGPSAPDRKECGLIIGGFNPVAADMVAARIAGFDFRRIPTLNKAFEI
                     QKLPLCQFKPSDITVTSNRDEFNDVIENLPTLPMYRLQPHFGWKGHIELNK"
BASE COUNT          646 a          431 c          414 g          716 t
ORIGIN      
        1 tagtacgttc cgtgccggcg ctgatcagag aatacatcgt gcgtacaaga atgccgccgg
       61 gttgtatggt cggtacggga acattatcaa ctttgagttc gccggtgtta taattttgaa
      121 taacctgaag caagagggag ttcctattat tatttaatat tttgaattaa aaatctgaat
      181 ttgccggagc gacccgattc tatttgttgc gtatctacca atttgatttc aagttcgata
      241 tcggatggat caagtttagc tgtgagtgta cttttaatgt cggcaatatc tgaattttgg
      301 aaggttttct tcggaactaa ttttaagatt agtctgccga tctcagattg ctcaaattga
      361 tattgggcga cattttcaaa tactggagta tgcatattta aggcggtaac cgagatcaaa
      421 ttttttttct ttgtgaagat ttgttcttgt aaccaacgtc cttcgactct ttttaataaa
      481 cgataattgc gtccacaaga gcatttttca tcagaatatt cggcaaagtc tccagttcga
      541 taacgaataa acggcatgat gtctcgatca aaccctgtac caactatttc gccaagggtg
      601 ccttcagaat caattatttt gccattggcg tcaataagtt caacataacc atattcagga
      661 aaaatatgat acttgtgaga atattcgcat tctccggcca atgcaaccat ttcgcccagt
      721 ccataccagc tgaatattct acactgaaac gcattttcca gtaactcgcg ttggcctgga
      781 taaaatgctt cagaactaca tagtacggcc ttaagcgtcg ggaacggggg aatatggtgg
      841 gctaccataa attttgcaag tacggtcacg gccgatggat atgtatgaat aaattgaggt
      901 ttaaattgcc gtattttttc aatataagtt gttaaactat tttcgttcat gtgataggta
      961 gacaaaatga ggcgattttt gattggctca tattcccaaa aaacttgatt tttcgctgaa
     1021 gggataacgt taccgcgtaa tgtgacaatg cgatcattga cttggtatcc aactcttttc
     1081 cattgtgatg tcatgaaggc aaactctttt gaacgtgaaa cgcctttttc attaaaaaag
     1141 ctcatgggaa tgccggttgt tccgcctgtg ctatagtata acaatttgga ttttgagtaa
     1201 tttgatccta ccattgaatc cttattggat aagatcaatt ctttcgttaa gaagggtatc
     1261 tttttgatat cttccaagga ttgaaaatca tttcttgtaa gattatgtag tctaaatagt
     1321 ttttcgtaat atggtacatt ctcaaaacag tgattgatga gcgtaagaaa ttttttgttt
     1381 tggtattcga cgatttgttc tttactccaa aactctgatt ggctcaaaag tgtcttgaag
     1441 tgtatatagg ttttcccata tctcaaacgt aaagggatat tcctatagac ccggcctaat
     1501 acgcttttaa cgaattgagg gctttttaag tataacccaa gaaatttgta aagtctgtcc
     1561 tcaatcatat tatttattca actcaatgtg ccccttccac ccaaagtgag gctgaagccg
     1621 atacataggc agagtaggta agttttcgat tacatcattg aattcatcgc gattagaagt
     1681 aactgttatg tcggatggtt taaattgaca cagaggtaat ttttgtattt caaatgcttt
     1741 atttaaagtg ggtattcttc taaaatcaaa tcctgcaatg cgtgcagcaa ccatatcagc
     1801 cgctactggg ttaaagcctc caatgattaa gccgcattct ttacgatctg gcgccgaagg
     1861 accattattt tcaccagcca caataccatc aattacagtc aaataccgtc gcttcgaagg
     1921 caacatcttt cctgacgcat tatagtaaaa taatatctta tttaaatcta aaaccattct
     1981 ccacaaggta tcattaccat accaatttcc gcttcgaatg acattatcgg tgcgaccgaa
     2041 aatggcgtaa cccaacttaa tagcaagttt cgcacctact cctcccgtac ctccaaaaaa
     2101 agttaaagct cgcttatata ttcttactaa ccatccttga aacgaatttc cgactgaaga
     2161 cttttcaaac tcatcaccgc cttgatcagg cgtgcccata gtatggt
//
LOCUS       DAOQGF010000242         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_32916_length_2203_cov_7.818901, whole genome shotgun sequence.
ACCESSION   DAOQGF010000242 DAOQGF010000000
VERSION     DAOQGF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32916_length_2203_cov_7.818901"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..820)
                     /locus_tag="PK937_16340"
     CDS             complement(176..820)
                     /locus_tag="PK937_16340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNH34194.1"
                     /translation="MTKLISALIFALLWIKPSLVCGQNNLELDSSISVLNLQKARWEY
                     DRTTLWRRLIPQITAGVSLSKSQYLLPAYDEYSNQPSLLGRGVYHITLSWNINDLIFA
                     DTRKRAYFALRNSEQLAHMIADRARRDEHSAQHKLLNQMRDIENQRTVISYVEQELIV
                     HRQLLELTQIKFNQAEKTAEDVFQRRLAVLNAERKLAEERAKLELLLNEGMPTE"
     gene            complement(817..1761)
                     /locus_tag="PK937_16345"
     CDS             complement(817..1761)
                     /locus_tag="PK937_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNH34195.1"
                     /translation="MGVEVFQMHSNDELTSQYLDYLKLEREASPCTLENYGYDIRHFV
                     NFVVPKNLLETTMSDVRRYVSFLKLTKGLANTSINRKLISLRSMFSFFVSIEVVSQNP
                     FVAFNYIGIRRKKPLSLKSNQLIHIMNHLDSECRIADESGDELKRFVAFRNRLMIKIM
                     ILSGFRRSEVITLKRSSLVKSDSSWAFSIMSRTPDNTRIVPVHSGIYSNLIEYLKMHR
                     GISDFLFVQSKTGRPLSVDTLYLIFKDIRAAIPGLSRIMPKSLRHTFASRLAANGESL
                     RTIQDLMGHKKIETTQIYIHVPDKTRAQAVNRLTLALQ"
     gene            complement(1811..>2203)
                     /locus_tag="PK937_16350"
     CDS             complement(1811..>2203)
                     /locus_tag="PK937_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34196.1"
                     /translation="TYVRTDKYVSYSDFLNNIPADTRETSVVGEADGTIRTVSIHNEQ
                     ETTYTFRSPIETVNGNKTTRRYGNDEGKVVTESRQNATLVSTRLIYGRSNGATVARTE
                     YPDNSWIQTENLGLADGTIRRTTSSGTN"
BASE COUNT          587 a          470 c          511 g          635 t
ORIGIN      
        1 aaaacacatc agtaaaagac gcgtacgcga cggaaacaat gcctgttaca ttcttatcct
       61 gactgcaagc aagatgcagg gggtccatga acgtcaaagg aatgtctttt atgatgtgat
      121 ttgcacctgt aacgttgagc atataacttc cctcttgata caccgccatt gacggctact
      181 cggtgggcat tccttcattt agtagtaatt ccagctttgc cctttcttcg gcaagtttgc
      241 gttcggcgtt tagaacagct aatcggcgtt ggaatacgtc ctccgccgtc ttttccgcct
      301 gattgaattt tatttgtgtc agctctaaaa gttgtctgtg tacaatcagc tcttgttcga
      361 cataactaat gactgttcgt tgattttcaa tatctcgcat ctgatttaat agtttgtgct
      421 gtgcgctatg ttcatcacga cgcgctcgat ctgcgatcat atgtgctaac tgttcagaat
      481 ttcgcaaagc gaaatatgct ctctttcggg tatctgcgaa aatgagatca ttaatgttcc
      541 aagacagcgt gatatgatag actccgcggc cgagcaaact tggctgattg ctatactcat
      601 cataggccgg caagaggtat tgagatttcg aaagcgatac gccggctgtt atctgaggta
      661 tcagacgacg ccacaatgtt gtgcgatcat attcccatcg agccttttgt agatttaata
      721 cgctgatgga agaatctaac tcaagattgt tctggccaca aaccagtgaa ggtttgatcc
      781 acaatagagc aaaaatcaaa gcacttatta attttgtcat tgtagagcca gtgttaatcg
      841 gtttaccgcc tgtgcccgcg tcttgtccgg aacgtgaata tagatttgag tcgtttctat
      901 tttcttgtga cccatcaggt cttgtattgt tctcaaactt tcgccgttgg cggcaagacg
      961 cgatgcaaat gtgtggcgga gactttttgg cataatacga gatagcccgg ggatggcggc
     1021 gcgaatgtcc ttaaaaataa ggtatagtgt atccaccgaa agtggccgtc ccgtcttaga
     1081 ttgaacgaaa aggaagtccg aaatcccacg gtgcattttc agatattcaa taagattaga
     1141 atagatgccg gagtggactg gaacaatccg cgtattgtcg ggtgttctac tcataatgga
     1201 aaaagcccaa gacgaatctg acttgacaag tgaagaacgc tttaaggtga tgacttctga
     1261 acgcctgaaa ccggatagga tcatgatctt aatcatcaaa cgatttctaa atgcgacaaa
     1321 tcgttttaat tcatcgcctg actcatcggc tatacggcac tcggaatcaa ggtggttcat
     1381 gatgtgaatg agctgattac ttttgaggct tagcggcttt ttccgacgaa tcccgatata
     1441 gttgaaagca acaaatgggt tttgagagac cacttcaata ctcacaaaaa aactgaacat
     1501 tgatctgagt gaaatgagtt tacgattaat ggaagtattg gctaatcctt tggtcaactt
     1561 caaaaaggac acataccggc gaacgtcact cattgttgtt tccagaagat tcttgggtac
     1621 aacaaaattc acaaaatgac gaatatcgta tccatagttc tcaagcgtac agggtgaggc
     1681 ttcgcgttcg agcttgagat agtcgagata ttggctggtt aattcgtcgt tggaatgcat
     1741 ttgaaacacc tccacaccca cccgtaccct cccttagcag ggcgggcaaa gacgagaatg
     1801 ctttggcaga ttagttagtg cctgaagacg ttgtacggcg aatcgttccg tcggcaaggc
     1861 cgagattttc agtttgaatc caagagttat cgggatattc ggtacgcgca accgtcgctc
     1921 catttgaacg gccgtagatc aaacgagtac ttacgagtgt tgcattttgg cgtgattcgg
     1981 taacaacctt accttcatca ttgccatagc gacgggtcgt tttgttgcca ttaactgttt
     2041 cgataggact acgaaacgtg taggtcgtct cttgttcatt gtgaattgag acggttcgaa
     2101 tcgtaccatc tgcttctccg actactgagg tctcccgggt atcggcagga atattattca
     2161 agaagtccga atagctcacg tatttgtccg tgcggacata tgt
//
LOCUS       DAOQGF010000243         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_33326_length_2187_cov_4.133208, whole genome shotgun sequence.
ACCESSION   DAOQGF010000243 DAOQGF010000000
VERSION     DAOQGF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33326_length_2187_cov_4.133208"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="PK937_16355"
     CDS             complement(<1..1747)
                     /locus_tag="PK937_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNH34197.1"
                     /translation="MAHKTTKRTLSKKKSKKTTLRKSDSTRQHDALLHLYRLRKGFDS
                     LVRAAVRISADLQSIDRVSVWLFTPDRSAIQCVALYSREKKKYFQGDVLLASKYKPYF
                     KALNSGRSLLAKNAHKDPRTSAFTEDYLLPNGIGAMMDVPIWQDGRVIGVLCHEHVGR
                     SRRWTQREEEFANAVADCITGSKHAEERRQAEVRYHALLEASDDMVFIEDYHRSVLYI
                     NPAVEKHTGYTAKDYDFNVHQHPLIHPEDLPNVNRFIADFIQSETMFSKTLENRFVDR
                     WGRLRWHSSVLSKIEYLQKPAIQFVVSDITGQKFAIDALHRSEERFTMLVRQLRDLIT
                     IVAADGTIRYESPSVFPMLGYAQDELLGRNAFDRIHPEDLDVLRKTFEKLLRAPGETA
                     TAMYRFLHKNGTWRHCESVGVNLLLDASVEGILITTRDVTERVEAEQLLKEREERYRK
                     LVESFHDILFITDFKRNMLYINPAMERLTGYTLNDFRFPPDDNPFVHPEDFKRVNAFI
                     DEFAQSAKTHSGIIENRFFDRWGKMHWYSSVISKIEYDDQPCLQFICRDISDVRRSEE
                     ALTQSDQKYRAFIEQS"
     gene            complement(1751..>2187)
                     /locus_tag="PK937_16360"
     CDS             complement(1751..>2187)
                     /locus_tag="PK937_16360"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNH34198.1"
                     /translation="QKEFNAFIDLKDRQLIGSGLRYEIMRTDSGRKSPVLVWAVGAGL
                     MWEREMYEHNEPTTRKLRSTNYLSIRWNPDDRLAFVATTYYQADTRKFSDYRLLHNTN
                     MSIALWKKLSFVAAFNARYDHRPAPGIRTYDMELSNGVNIVF"
BASE COUNT          607 a          535 c          454 g          591 t
ORIGIN      
        1 tactttgctc gatgaaagcg cggtattttt gatcactttg ggtcaacgct tcttccgaac
       61 gacgtacgtc ggaaatatca cggcaaataa actgcaggca tggttgatcg tcgtattcta
      121 ttttggagat caccgacgaa taccaatgca tcttgcccca acgatcaaaa aaacgatttt
      181 ctataatgcc tgaatgagtc tttgcacttt gcgcaaactc gtcgataaac gcattaactc
      241 ttttaaaatc ttccgggtga acaaagggat tatcgtcggg cggaaaacga aagtcattga
      301 gtgtataacc ggtaagacgt tccatcgccg gattgatata cagcatattg cgtttgaaat
      361 ccgtgataaa aagaatatca tgaaacgact cgactagttt tctgtaacgc tcttcccgtt
      421 ctttgagcaa ctgttcagct tccacgcgtt cggtgacatc gcgtgtcgta ataagaattc
      481 cctcaacgga tgcatccaaa agcaaattga caccgacgct ttcacagtga cgccacgttc
      541 cgtttttatg caaaaagcga tacatggccg ttgccgtttc gcccggtgca cgaagcaatt
      601 tttcaaatgt ttttcgcaat acatccaaat cttcaggatg aatccgatca aaagcatttc
      661 gcccaagcaa ctcgtcttgc gcataaccga gcatcggaaa tacggaaggg ctttcgtacc
      721 ggatcgtacc atccgcagca acgatggtta tcagatcgcg caattgacgc accaacatgg
      781 taaaccgctc ttcgctgcga tgcaatgcat ctatggcaaa tttctggccg gtaatgtcac
      841 tgaccacaaa ctgaatggcc ggtttttgga ggtattctat tttactgagt acagacgaat
      901 gccagcgcaa tctcccccag cgatcaacaa aacgattttc caaagttttt gaaaacatag
      961 tttcgctttg gataaaatcg gctataaaac gattgacgtt tggcaaatct tcaggatgta
     1021 tcaaaggatg ttgatgtaca ttaaaatcat aatctttggc cgtgtatccc gtatgctttt
     1081 caacagccgg attgatatac agtacgctgc gatgatagtc ttcgataaac accatatcat
     1141 ccgacgcttc gagcaaagcg tggtatcgca cttcggcttg acgcctttct tccgcatgtt
     1201 tagaaccggt gatacaatcc gccacggcgt tagcaaactc ttcctcgcgt tgagtccatc
     1261 ggcgcgacct accgacatgc tcatggcaca aaacaccgat cacacggccg tcttgccaga
     1321 tcggtacatc catcatcgcg ccgatcccat tgggcaaaag ataatcttcc gtaaaagcac
     1381 tggtgcgggg atctttgtga gcattcttcg ccagtaatga ccggccggaa ttcaatgcct
     1441 taaagtaagg tttatattta gaagctaaaa gtacgtcccc ttgaaaatat ttttttttct
     1501 cccgactgta aagtgcgaca cattggatag ccgatcgatc cggcgtgaat aaccaaacac
     1561 taacgcgatc aatagattgc agatcggcgg atatgcgtac ggcggcacgc accaacgagt
     1621 caaaaccttt ccgcagacga tacaaatgca gaagggcatc atgctgccgt gtgctatctg
     1681 attttcgaag agtcgttttt ttgctcttct ttttagataa tgttctcttt gtggttttat
     1741 gcgccataac tcaaaaaact atgtttacgc cattgctcaa ttccatatcg taagtacgaa
     1801 tcccgggcgc cggcctatga tcataccgcg cattaaaggc tgccacaaaa ctcaattttt
     1861 tccatagcgc aatactcata ttagtattat gcaaaagacg atagtcagaa aacttgcgcg
     1921 tatccgcttg ataataggtc gtagcaacaa aagccaaccg atcatccggg ttccatcgta
     1981 tggatagata gttagtcgaa cgcagttttc gggtggtcgg ttcattgtgt tcatacattt
     2041 cacgctccca catcaatcca gcaccgacag cccataccaa tactggagac ttgcgccccg
     2101 aatccgtccg cataatttca tagcgtagac cactgccgat caattgacga tctttaaggt
     2161 caataaacgc attaaattct ttctgcg
//
LOCUS       DAOQGF010000244         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_33452_length_2182_cov_5.214857, whole genome shotgun sequence.
ACCESSION   DAOQGF010000244 DAOQGF010000000
VERSION     DAOQGF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33452_length_2182_cov_5.214857"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PK937_16365"
     CDS             complement(<1..650)
                     /locus_tag="PK937_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34199.1"
                     /translation="MLSKFRTFAVFNIVLFTCFISAIGAQTALPKDKIFRQLYFQPGD
                     WVTIGGTRYATCVASTFNMAYIGTLGGIIRYDIIRRQFLYPLTISDGLPENEIFLIGA
                     NSQSQLVWIITRHFICTFNPNTSRVQDVTPIEYLNVRDASTLDAIGFGAGSVWLRSED
                     RYWNSSLSYQSFSPSELPKKETIEWNNIPASSEIASRSIFVSNESGYIFIPHSNLF"
     gene            complement(661..2085)
                     /gene="gatA"
                     /locus_tag="PK937_16370"
     CDS             complement(661..2085)
                     /gene="gatA"
                     /locus_tag="PK937_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNH34200.1"
                     /translation="MHKTFSQISENLKSGATNCETIVSQYLDSIKSQSALNAFIHVLP
                     YEKTIARAKHIDAKVKSGQYGRLAGMVVAVKDAIVVKDENATAGSQILKNYVSPFSAT
                     VIERLENEDAIVIGKANMDEFAMGSSNENSSFGPALNPLDTSRVPGGSSGGSAVAVAA
                     GLSTTSLGSETGGSVRLPASFCGVVGLKPTYGRISRYGLIAFASSFDQIGPMGLTVED
                     TARLTHTIAGIDHRDATSASSPVPNYTDELKKGVKGLRIGIPKEYFGQGLDPEIRAGI
                     EKTIEKLSKEGAVFSDITLPHTEYGIATYYILVTAEASSNLARYDGVRYGHRSTKSVT
                     MADMFIKSRSEAFGEEVKRRIMLGTYVLSAGYYDAYYKKAQKVRTLIKKDFTQVFSQG
                     IDCILAPTSPTVAFKFGEKSNDPLAMYLSDVYTVNINIAGNPAISVPCGKNSENMPYG
                     VQIIGKDFDESMCFRVGAAIESLR"
BASE COUNT          612 a          489 c          501 g          580 t
ORIGIN      
        1 tgaaaaaggt tgctatgggg tatgaatata tatcctgatt cattactgac aaaaatggag
       61 cgtgaagcga tttccgatga cgccgggatg ttattccatt ctatagtttc tttttttgga
      121 agttctgatg gtgaaaatga ttggtacgaa agagaggagt tccaataccg atcttccgaa
      181 cgcagccaaa cggaacctgc accgaatcct atcgcgtcaa gagttgacgc atcgcgaaca
      241 ttcagatatt cgatgggtgt tacatcttgt acgcgcgatg tattgggatt gaaagtgcaa
      301 atgaaatgac gcgttatgat ccagaccaat tgtgactgag aatttgcacc gatcaaaaag
      361 atttcatttt cgggtagccc atcactgatg gtcagcgggt agagaaactg acgtcggatg
      421 atgtcatatc ggattatacc acccagtgta ccgatgtaag ccatgttaaa agtggaagct
      481 acacaagtag catatcgcgt accgccgatg gttacccaat ctccgggctg aaaatataat
      541 tgcctgaaga ttttatcttt gggtagagca gtttgagcgc caatagcaga aataaaacaa
      601 gtaaacaaaa cgatattgaa cacagcaaat gtacgaaatt tcgacaacat cggcctccga
      661 ctagcgtaat gattcgatcg cagcacccac gcggaaacac atgctttcat caaagtcttt
      721 accgatgatc tgtacgccat aaggcatatt ttccgaattt ttgccgcacg gtacgctgat
      781 agcagggtta ccggcgatat tgatattaac ggtataaaca tccgataaat acatggccaa
      841 aggatcgttg gatttttcac cgaacttgaa cgctaccgtc ggtgacgtcg gcgctaagat
      901 gcaatctata ccttgcgaaa aaacttgagt aaaatccttt ttgattagcg tacggacttt
      961 ctgcgctttt ttgtaatacg cgtcataata acccgcactc aacacatagg taccaagcat
     1021 gatgcggcgc tttacctctt cgccgaaggc ttcactgcga cttttgataa acatgtccgc
     1081 catcgtaacg gactttgtac tgcggtgacc ataccgcacc ccgtcgtaac gtgcaagatt
     1141 tgatgaggcc tccgccgtaa caagaatata atatgttgca atgccgtatt ctgtatgcgg
     1201 cagtgttata tcactaaaca cggcgccttc tttgctcagt ttttcaatcg tcttttcgat
     1261 acctgcacga atttcaggat caagtccttg tccgaaatat tctttgggaa taccgatacg
     1321 aagtccttta acaccttttt ttaattcatc cgtgtaattg ggaacggggg aagaagcgct
     1381 ggtcgcgtcg cggtgatcaa taccggcgat agtgtgcgtg agccttgccg tatcttcgac
     1441 cgtaagcccc atcggcccga tctgatcaaa ggaagaggca aacgcaatca ggccataacg
     1501 cgaaatgcgc ccatacgtcg gtttgagccc gaccacacca caaaaactgg ccggtaaacg
     1561 aaccgatcca ccggtttcag atcctagcga tgtcgtgcta agtcctgcgg ctaccgcgac
     1621 ggcgctgcca ccggatgaac cgccgggaac ccgcgatgta tctaaaggat tgagtgcggg
     1681 tccaaatgaa gaattctcat tggaggatcc catcgcaaac tcatccatat tggctttacc
     1741 gatgacgatt gcatcttcat tttcaaggcg ctcaataacc gtcgcggaaa aaggggagac
     1801 gtagtttttt aaaatttgtg aaccggccgt tgcgttttca tctttgacga cgatcgcatc
     1861 ttttacggca acaaccatac cggctaaacg gccatactga cccgatttga ctttagcatc
     1921 aatatgtttg gctcgtgcta tggttttttc gtacggcaaa acgtggataa atgcgtttaa
     1981 agcggattga gatttgatcg aatcgaggta ttgagaaacg atcgtttcgc agttcgtcgc
     2041 gccggatttt aaattttcag agatctgaga aaaagtttta tgcattgtga attaaatatg
     2101 gttatacaaa aaaaatcccg atcaccatgc ggcctatcgg gatagtgaaa aaacatgtta
     2161 aatcagctta gggtttacgt tc
//
LOCUS       DAOQGF010000245         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_34632_length_2139_cov_4.249040, whole genome shotgun sequence.
ACCESSION   DAOQGF010000245 DAOQGF010000000
VERSION     DAOQGF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34632_length_2139_cov_4.249040"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK937_16375"
     CDS             <1..440
                     /locus_tag="PK937_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34201.1"
                     /translation="RFLLLVLLLNVVRYIAAFPLEMMLVFPGLFGAMESAPGVFNVSF
                     TAGDWITSYVYNFILWTVSVFIFHRMAPVVGGKWIIKSVKIFFLPWLLFVSVSAIYMN
                     HYAHTKMFYVYTMLDSVITFGIVAVANGLLYPRIMGKNSSVAS"
     gene            549..773
                     /locus_tag="PK937_16380"
     CDS             549..773
                     /locus_tag="PK937_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNH34202.1"
                     /translation="MQKTAVRKIGNSLGITLPKTVLENFHLSEGDELHLIETNEGIIL
                     SPFNPDFSEWATAYDQVNRKYRNTLHKFAK"
     gene            773..1168
                     /locus_tag="PK937_16385"
     CDS             773..1168
                     /locus_tag="PK937_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNH34203.1"
                     /translation="MAVKYPPIELVKSIHANQIRQYGGIFGLRDPGLLESALAQPRRS
                     VGGKLIHKTIFDKAAAYGFHLCKNHPFVDGNKRVAFIIMYIFMANNNWIINASEESAY
                     SMMMELAEGKLSKRRLSAWLQKNSHKSLK"
     gene            1489..>2139
                     /locus_tag="PK937_16390"
     CDS             1489..>2139
                     /locus_tag="PK937_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNH34204.1"
                     /translation="MIRLDAITRNRENHVLIRFGFDEELIALVKQLPDIRWDSADKAW
                     QTPNTKENVNRIFLLFKGKAWIDYSSLKSQKPEIKPARKNAHVKIILSKQLNEEVLGK
                     LEQFERWMQSKRYGENTIKTYTDALRTFLRFHHSVPLDKITNDHIIKFNVEYITKGHY
                     SAAFQNQVVNAVKLFFSKIENTVLDLKSVHRPRREKLLPNVLSKQEVKAILDAPKNI"
BASE COUNT          627 a          417 c          453 g          642 t
ORIGIN      
        1 tccgatttct tttacttgta ctgctgctca atgtcgtccg gtatatcgcc gcgttccctc
       61 tggagatgat gctcgttttc ccggggcttt ttggtgcgat ggagtccgcg ccgggtgtgt
      121 ttaatgtgag ttttactgcc ggagactgga tcacatcgta tgtgtataat tttatcctct
      181 ggacggtaag tgtttttatt tttcatcgca tggccccggt cgtcggcggc aaatggatca
      241 tcaaaagcgt taaaatattt tttctgccgt ggttgctatt tgtaagtgtc agtgcgatct
      301 atatgaatca ctacgcacac actaaaatgt tttatgtcta tactatgtta gacagtgtga
      361 ttacgtttgg tatcgtcgcc gtggccaatg ggttattata cccgcggatt atgggaaaaa
      421 attcgtcggt cgcttcgtaa actgccgttt cctgtttttt atgtaagatc atgccgccgg
      481 tatggatagc caaaacttga cattacgtaa tacacttgtt ataatataac aagtgaaagg
      541 atcaagtcat gcaaaaaacc gcagtacgca aaattggaaa tagtcttggt atcacactgc
      601 ctaaaactgt tctcgaaaat tttcatctct ccgaaggcga tgaacttcat ttgatcgaga
      661 caaatgaagg catcattttg tcgccattta atccggattt ttcggaatgg gccacggcgt
      721 atgatcaagt taaccgaaaa tacaggaata cgctgcataa gtttgccaaa taatggctgt
      781 aaaatatcct cccattgaac ttgtaaaatc aattcatgcc aatcagattc gacaatatgg
      841 cggaattttt gggcttcgtg atcccggact tttagagtcg gctctggcac aacctcgaag
      901 gagtgtgggt ggcaaactaa tccataaaac gatttttgat aaagccgccg cgtatggctt
      961 tcatctttgc aaaaatcatc ctttcgtgga tggaaacaaa cgcgtcgctt ttatcattat
     1021 gtatatattc atggccaaca ataattggat aatcaacgct tcggaagagt ctgcttacag
     1081 tatgatgatg gagcttgcgg aaggtaagct ctcaaaacgc aggctctcag cctggctcca
     1141 aaaaaactcc cacaaatcac tcaaataaca cggcggaata ccttatagtg ttatgcggct
     1201 tggcggattt ttaccgcaaa agtttaatag aattacggcc tttgaacctt gcacgaatat
     1261 tttattgaag cgcgctttca tttctctttt gaaaatcggt acggttcggc tgtatcaaaa
     1321 tgatgtttga aaaacttttt tctcgatcgc gtttctataa atagtcattg acaaatgaaa
     1381 cgcgtacatg tacatttaag catgtagcct gtctggtatg ccacgtctcg aagggttctt
     1441 tggttaactt atatttttga catgatgcat agccatttga aaaataaaat gattcgttta
     1501 gatgcgatta cccgcaacag ggaaaatcat gttttgatca gatttggttt tgacgaggaa
     1561 ctgatagctc ttgtaaaaca acttccggac atccgatggg actctgccga taaggcttgg
     1621 caaacgccga ataccaaaga aaatgtcaat cgcatttttt tactttttaa gggtaaagcg
     1681 tggattgatt acagttcatt gaaatcccaa aaaccggaaa taaaaccggc acgaaaaaat
     1741 gctcatgtta agattatttt atcgaaacag ttgaatgagg aggttctagg aaagcttgag
     1801 caatttgaga gatggatgca gtccaagcga tatggtgaaa acacgatcaa aacctacacc
     1861 gatgcgttac gaacattttt gcggtttcat catagcgtac ctcttgataa aatcaccaat
     1921 gatcacatta ttaagtttaa tgtcgaatat atcacgaagg ggcattattc ggcggccttt
     1981 caaaatcagg tggtcaacgc cgtaaaatta tttttcagca aaattgaaaa caccgtatta
     2041 gatttgaaaa gcgtccatag gccaaggcgt gaaaagttat tacccaatgt actcagtaag
     2101 caagaggtta aagccatttt agacgcgcct aaaaatatc
//
LOCUS       DAOQGF010000246         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_34856_length_2131_cov_8.148362, whole genome shotgun sequence.
ACCESSION   DAOQGF010000246 DAOQGF010000000
VERSION     DAOQGF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34856_length_2131_cov_8.148362"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK937_16395"
     CDS             <1..495
                     /locus_tag="PK937_16395"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNH34205.1"
                     /translation="NKIPVLRGVDLIAIPLPLGYGIGRIGCHLSGDGDYGLPWDGPWA
                     TNYSRGVVPPSQAFHGTEIARQYPNGIVPDNTLCHPTPLYETLISTGIFLILWHMSKR
                     KLPAGSIISLYLMLAGMERFFIEFMRLNPKVAFGLSGAQLISVMMITVGASSLAYLWK
                     RATA"
     gene            492..1172
                     /locus_tag="PK937_16400"
     CDS             492..1172
                     /locus_tag="PK937_16400"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNH34206.1"
                     /translation="MIRFVYVLFLILVLNDTVSAQANGKIASSRFTIARLKYGGGGDW
                     YGNKNALNHLLRYLSDNTNIAVEKTETYVDPSDIRLFDYTFLYMAGHGNVKFSDDDVR
                     QLRRFLTGGGFLWCDDDYGIDPYFRREMKRVFPELDFTLIPFSHPIYSIGSRFPNGMP
                     KIHEHDGGPPQGYGLFWKGRLICFYSKNTDISDGLEGPEVFPEDHPEKQAEALRFAAN
                     IVMYALSY"
     gene            1186..1881
                     /gene="ccsA"
                     /locus_tag="PK937_16405"
     CDS             1186..1881
                     /gene="ccsA"
                     /locus_tag="PK937_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNH34207.1"
                     /translation="MKNGVGEKILDAAMLVVMLAALYLTFIWVPNEKTMGIIQRVFYF
                     HVPAAWVSFLAFGFVFVASIMYLIKRDDKWDAMAVCSAEIGLLFCTIVLVTGPIWAKP
                     VWGIWWTWDARLTLTLVLWLIYLAYTMLRAYIDDPLKRGRLSAVVGIVGFVDVPLVYF
                     SIRWWRTQHPAPVMAGGEGSGLAPEMRMVLMFCLAAFTLLFVYLLNKRLKMEKTEREL
                     ELLYRTVQRDNRM"
     gene            1929..>2131
                     /locus_tag="PK937_16410"
     CDS             1929..>2131
                     /locus_tag="PK937_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34208.1"
                     /translation="MRKIILIVCLLLLRTTVFAGDGYQFLRVGVTARATGLGDVFVAQ
                     PGDATTFMYNPAGLATLQGRTFAA"
BASE COUNT          462 a          455 c          582 g          632 t
ORIGIN      
        1 aataaaatcc ccgtcttgcg cggcgtggat cttatagcga tcccgttacc gctcggttat
       61 ggtatcggcc gtatcggatg ccatctttcg ggcgacggcg attacggttt accgtgggat
      121 gggccttggg ctacaaatta ttcacgcggc gtcgttccgc cttcacaggc ctttcacggc
      181 acggagatcg ctcgccaata tccgaacggg atcgttccgg acaataccct ttgccacccg
      241 acgccgttat atgaaacgct gatttcaacg ggaatttttt tgatcctgtg gcatatgagt
      301 aaacgtaaat tgcctgcggg ttccatcatt tcgctctatc tcatgctcgc cggtatggag
      361 cgttttttta tcgaatttat gcgtctcaat cctaaagtgg cgtttggcct gagcggcgcg
      421 caattgatca gtgtgatgat gattacagtc ggtgcgagtt cgctggctta tttgtggaag
      481 cgtgcaacgg catgatacgg tttgtttatg ttttattttt gatcttagtg ttaaacgata
      541 ccgtatcggc acaggccaat ggaaaaatcg cgtcatctcg ttttacgatc gcgcgcctca
      601 aatacggcgg cggcggggat tggtatggca ataaaaacgc attgaatcac ctgctgcgtt
      661 atttgagtga taataccaat attgccgttg aaaaaacgga gacctacgtg gatccgtcgg
      721 atatccggct ttttgattat acctttttgt atatggccgg tcacggcaac gtcaaattca
      781 gcgatgatga tgttcgccaa ttgcgtcgtt ttctgaccgg cggcggtttt ctttggtgcg
      841 atgatgatta tggtattgat ccgtattttc ggcgtgagat gaaacgtgtg tttccggaac
      901 ttgattttac gctgattcct ttttcacatc cgatttattc gatcggatca cgcttcccca
      961 atggtatgcc gaaaatacac gaacacgatg gaggcccccc gcaaggttac ggattgtttt
     1021 ggaaagggcg attaatttgt ttttattcta aaaatacgga tatcagcgac ggcctcgaag
     1081 ggccggaagt atttcccgaa gaccatcctg aaaaacaagc ggaggcactt cggtttgcgg
     1141 cgaatattgt aatgtatgcc ttaagttact gagaaggtga agaatatgaa aaatggtgtc
     1201 ggtgaaaaaa ttcttgatgc ggctatgttg gtggttatgc tggcggcgct gtatctgacg
     1261 ttcatttggg ttcccaatga aaagaccatg ggtatcattc aacgcgtgtt ttattttcat
     1321 gtgcccgccg cgtgggtttc atttttagcg tttggttttg tttttgtcgc gagcatcatg
     1381 tatctgatca agcgggatga caaatgggac gccatggccg tatgttcggc ggaaatcggt
     1441 ttgctttttt gtacgatcgt gttggtgacc gggccgattt gggctaaacc ggtgtggggc
     1501 atttggtgga cgtgggatgc gcgcctgaca ttgacgttgg ttctgtggct tatatacctc
     1561 gcatacacga tgttacgggc atacattgac gatccgctca agcggggacg cctttcagcc
     1621 gtcgtcggca tcgtgggatt tgtagatgtt ccgctggttt atttttcgat tcgctggtgg
     1681 cgtacacagc atccggcacc cgtcatggcg ggcggcgaag gttcgggttt ggcaccggaa
     1741 atgcgtatgg tgctgatgtt ttgccttgcc gcatttactc tgctttttgt gtatcttttg
     1801 aataaacgcc tgaaaatgga gaaaaccgaa cgcgaacttg agttacttta tcgtacggtg
     1861 caacgggata accggatgta gttttttgcg atagcatagt tggcgccaag acgttatatt
     1921 aaaaaacgat gagaaaaata attctaatag tttgtttgtt gctgctccgg acgaccgtgt
     1981 ttgccgggga cgggtatcaa tttttgcgtg tcggcgtgac agcccgtgcg acgggactcg
     2041 gcgatgtgtt tgtcgctcaa cccggtgatg caacaacgtt tatgtacaat ccggcaggcc
     2101 ttgcaacact acaagggcgg acttttgcag c
//
LOCUS       DAOQGF010000247         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_36025_length_2090_cov_3.432432, whole genome shotgun sequence.
ACCESSION   DAOQGF010000247 DAOQGF010000000
VERSION     DAOQGF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36025_length_2090_cov_3.432432"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK937_16415"
     CDS             <1..581
                     /locus_tag="PK937_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34209.1"
                     /translation="TVSPRLFALLIKANSLGDTVWTKRYHSLTDPATGLIPNRDIIRA
                     LVFENSGHIVIAASSLLSFPHYGHTDFWFVRVDTDGNIKLQTKYREQFRDDVFPILLP
                     HALSRLGNGDYVISGRFYDRFVGPRYQMWVARFTSQGNMIWSKSFGDVLDDEGLGVEV
                     IGNSIYSTGYTMTDSLSTDIYLLRLDLNGEIR"
     gene            complement(897..1886)
                     /locus_tag="PK937_16420"
     CDS             complement(897..1886)
                     /locus_tag="PK937_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNH34210.1"
                     /translation="MPANLSTSYMGFTLKNPIVPSASPLSKHLDNIRKMEDAGAAAVV
                     LFSLFEEQIYHDAMELVQEASKISEDFRNSFDYLRELNQSHYNPDAYLEHIRKAKEIV
                     KIPIIASLNGSSDNGWLQYAKSIEAAGADGLELNLYHIAADPKKNSAQIEDQFIHVVR
                     SVKNLKIPVSVKLSPHITGLANFVSRLDETRVDGVVLFNRFYQPDFNLNSLKVVPNIN
                     LSDSTDLRLALRWTAILYGQVHAPIAATSGIHSHEDAIKMIMAGASVTMVCSALLKNG
                     ISHITELLRDIELWMDIHECQALSHIRGIMSQKSKDDPSAMERAQYIKALAQY"
BASE COUNT          582 a          401 c          491 g          616 t
ORIGIN      
        1 ataccgtatc acctagacta tttgcattat taattaaagc aaatagtcta ggtgatacgg
       61 tatggacaaa aagataccat agtttgaccg acccagctac gggcctaatc ccaaatcgtg
      121 acatcattcg tgcactggtt tttgaaaatt ccggtcacat tgtcatagca gccagttcat
      181 tactttcgtt ccctcactac ggccataccg atttttggtt tgtacgcgtt gatacggacg
      241 gtaatattaa gcttcaaacc aagtacagag agcagtttcg cgatgatgtt ttcccaattc
      301 ttcttcccca tgcgttgtcc cgcttgggga atggcgacta tgttataagt gggcggtttt
      361 atgataggtt cgtcggtccg agatatcaaa tgtgggtagc gcgttttaca tcgcaaggta
      421 acatgatctg gagcaagtcg ttcggtgatg ttttggatga cgaagggttg ggcgtagaag
      481 taataggaaa ttcaatttac agtaccggct atactatgac ggattctctt tctaccgaca
      541 tctacctatt aagactagat ttgaatggag aaatcaggta gctacacgca tattactgat
      601 atgggaaatt tatgttacgc ggtattatgt gctcaagtaa gtagtgttca ttcatcgaat
      661 ctgatcagag tgtgaatgtg aagaagttat aatgtttcga tgaatcttga ttttagaagc
      721 actgatattg tcaatgtata gtgtggaaaa tccacgagta gaatttatat cggatgtcca
      781 cgaattgata cctacgtaat tataatcttt taaattaaga attttatgaa acggtgttca
      841 actctgagtt catgacaccg actacgatcg gtaaagcaaa tcattgatat agatcgctaa
      901 tactgagcca acgctttgat gtattgtgcg cgttccatgg ccgatgggtc gtctttggat
      961 ttttgactca tgatgccgcg gatatggctc aatgcttggc actcgtgaat atccatccaa
     1021 agttctatat cgcgcagtaa ttctgtaatg tgagagatgc cgtttttgag taaggcggaa
     1081 cacaccatcg ttaccgaagc gccggccata atcattttga tcgcatcttc gtgggaatgt
     1141 atgccgcttg tcgctgcaat cggggcatgc acctgaccgt acaggatcgc tgtccagcgc
     1201 aaagcaagcc gtaaatcggt tgagtcacta agattgatat taggaacaac tttgagtgaa
     1261 ttaagattaa aatcgggttg ataaaagcga ttaaaaagaa cgacaccgtc cacgcgcgtt
     1321 tcgtccaacc gggaaacaaa gttagctaac cctgtgatat gagggctgag cttgacagaa
     1381 acagggatct taagattttt gacggaacgg acgacgtgta tgaattgatc ttctatttgc
     1441 gcagaattct ttttaggatc ggcggcgatg tgatacagat tgagttccag cccgtcagcg
     1501 cctgctgcct caatggattt ggcgtattgt aaccaaccgt tatccgaaga tccgttgagg
     1561 ctggcaatga taggaatttt aacaatttct ttggctttgc ggatatgctc aagataagca
     1621 tcaggattat aatgcgactg attgagttcg cgtaagtaat caaaggagtt tcggaagtcc
     1681 tcacttattt tggaggcttc ttgtactagc tccatcgcat cgtgataaat ctgctcttca
     1741 aacaacgaaa atagtacgac agccgccgcg cccgcatctt ccattttcct gatattgtct
     1801 aaatgtttag atagcggaga agctgaagga acaatcgggt tttttaacgt aaaacccatg
     1861 tacgaagtgg ataaattcgc cggcatatat gaaaacctcg aagatggttg gacgtggtaa
     1921 attaaaacat gcggcattta tatacaagga tgttccttgg tgttgacaat tacggaattt
     1981 tttacgacca tgcgacgttc taagaatgta tctcagagat accaatgttc ttttgcaata
     2041 tggggacgaa ctggattcga cgggtatagg gtgctttggg tcgcatgccg
//
LOCUS       DAOQGF010000248         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_37214_length_2050_cov_5.573935, whole genome shotgun sequence.
ACCESSION   DAOQGF010000248 DAOQGF010000000
VERSION     DAOQGF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37214_length_2050_cov_5.573935"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK937_16425"
     CDS             complement(<1..516)
                     /locus_tag="PK937_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNH34211.1"
                     /translation="MKLDSKIPSGPLADKWSQHKFNMKLVNPANRRKYTVIVVGTGLA
                     GASAAATLGELGYNVHSFCIQDSPRRAHSIAAQGGINAAKNYSNDGDSVHRLFYDTIK
                     GGDYRAREANVHRLAELSVNIIDQCVAQGVPFAREYGGQLDNRSFGGAQVSRTFYARG
                     QTGQQLLIGAYQ"
     gene            complement(575..1270)
                     /locus_tag="PK937_16430"
     CDS             complement(575..1270)
                     /locus_tag="PK937_16430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNH34212.1"
                     /translation="MQVPFSTLVTSSVGKKIMTGLTGLGLCLFILSHLLGNFNLFLGP
                     DAFNQYTKTLESFGKALYVIEALLVLAFVMHAVVGISVWQSKRRARPEAYHKTAYAGG
                     NSKKSFASLSMLYTGLIILLFLFIHIKSLKFGPNMAEGYVYTHTVTGEKMRDMYKLTV
                     EHFKNPFYAFGYALVMLLLGVHLRHGFWSAFQSLGLTHQRLKPLIFSVGILFASAMAL
                     GFFVLPLWIYFSR"
BASE COUNT          602 a          407 c          488 g          553 t
ORIGIN      
        1 ctggtaagcg ccaatcagga gttgttgccc ggtttggccg cgggcataaa atgtacgcga
       61 tacctgtgca ccgccaaagg agcggttatc tagttggccg ccgtattcac gtgcaaacgg
      121 gacgccttgt gcgacgcact ggtctataat attaacggaa agctccgcta aacgatggac
      181 attagcttcg cgtgcgcggt aatctccgcc tttgatcgtg tcataaaaca aacggtgcac
      241 gctgtcgccg tcgttggaat aattttttgc cgcattgatg ccgccctgcg ccgcgatcga
      301 gtgagctcgg cggggcgaat cctgaatgca aaaagaatgt acattgtagc cgagttcgcc
      361 gagggttgct gcagcggatg caccggcaag tcccgtaccg acaacaatga cagtatattt
      421 acggcgattc gcaggattga ccaacttcat attgaactta tgttggctcc atttatccgc
      481 gagcgggcct gagggaattt tggaatcaag tttcatgagt atcctcgtaa gcttatatag
      541 tcattaggat gcagatatta tcgtaaagca tgtcctaccg tgaaaaataa atccacagcg
      601 ggagcacaaa aaagcccaac gccatcgctg aggcaaaaag aattcccacc gaaaaaataa
      661 gcggcttcaa acgctgatgc gtgaggccca gcgattggaa ggcgctccaa aatccatgcc
      721 ggaggtggac gccaaggagc agcattacca acgcatatcc gaacgcataa aacggatttt
      781 taaagtgttc gaccgtcagt ttgtacatgt ctcgcatttt ttcgccagtg actgtgtggg
      841 tgtagacata cccttcggcc atattgggtc caaacttaag cgatttgatg tgaataaaaa
      901 gaaacagaag aataataaga ccggtataaa gcattgaaag cgaagcaaag gattttttgc
      961 tgttgcctcc ggcgtaagcc gtcttgtgat aggcttcggg acgcgcgcgc cgtttgctct
     1021 gccatacgga gatgcctacg accgcgtgca ttacaaaggc caaaacgagc aaggcttcga
     1081 tcacatacag cgctttgccg aaactttcca gggttttggt gtattgattg aaagcatccg
     1141 gaccgagaaa aagattaaaa ttaccgagga ggtgagaaag aataaaaaga cacaaaccca
     1201 accccgtcaa gccggtcatg atttttttgc cgacggagga ggttaccagc gtcgaaaagg
     1261 gaacctgcat atagtaaact ccggaaacgt ggtaaataat gatattatga agaaacgaat
     1321 tgctgtcgga atgaacaaag gcaaaatacg ggaatgaaaa aaaaaatgca aggggattgt
     1381 attgattaaa cttgcgctac gattattgct tcgctggata agatgctccg ataaaataat
     1441 gatcattggc ttttaaatga acgcatggct ttaatagttt acatgagtga tggtgaattt
     1501 tataataacg atttcttatg atttcttcag gtttttagct atttggtctt ttttgagcac
     1561 aaacagcacg taaaatctta ataaataatc attcaaaatt aacttcgttt tttgtttaga
     1621 taatgttggt aattttttat taacaaaatt gttggcaatt cgatatcgtt attatttata
     1681 gttaataaat ataaatattt atcatgttga taacttgtta ttatcttgaa atgcaaaaat
     1741 tcggcttgcg tttttttagg tgggacttta ttatgtgctt acccctgttt ttgagagcga
     1801 catggctttc ggttcgtcca caggagttga gaggccggtg gacatcggga aggttgtctg
     1861 aaaagacgat tcagacctac ggttcttttg tcccttctac agacggctcg taagacagga
     1921 aaaaacgttt ttaataagtt ttttataact gtttatcaaa ccttattcta aaggaatctt
     1981 ggagccatgg caattcaaga gcgtgatgtg aaaaaagcat ctcccaagaa ggcggatgcg
     2041 gcgatgctca
//
LOCUS       DAOQGF010000249         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_37658_length_2036_cov_6.701161, whole genome shotgun sequence.
ACCESSION   DAOQGF010000249 DAOQGF010000000
VERSION     DAOQGF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37658_length_2036_cov_6.701161"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..805)
                     /locus_tag="PK937_16435"
     CDS             complement(239..805)
                     /locus_tag="PK937_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNH34213.1"
                     /translation="MIAKLFRWFFRMKGWTITAPIAPNIKKCVIIGAPHTSNWDFVYG
                     IGALECFNMRVNFLAKKELFRFPLKGMFLRMGGIPVDRSGKNSMVDAMIDVFKAREDL
                     MIIIPAEGTRKRVEKWKSGFYHLALGANVPIVLGFLDYEKKLAGFGPALYMTGDEIGD
                     MAKIRDFYKDKTGKIPEFFNIDAVRLSR"
     gene            complement(859..1167)
                     /locus_tag="PK937_16440"
     CDS             complement(859..1167)
                     /locus_tag="PK937_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34214.1"
                     /translation="MPKKSPVQSKKRVRVKGLGWKRSVAIDSDKYKKVSKAILSVLTT
                     SPIRFTEIVRLVEKKIPDFEGSLSWYTISVARELEAQGKIIRHPKPVLYSKPRRTIQK
                     "
     gene            complement(1218..>2036)
                     /locus_tag="PK937_16445"
     CDS             complement(1218..>2036)
                     /locus_tag="PK937_16445"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNH34215.1"
                     /translation="ELQKFLTWSEGRSPLVSAHRGGPVSGFPENCLATFENTIKYTYA
                     LIECDVRISKDGNLVMMHDKTLDRTTTGKGPVSDMLFADLKQLRLKDNDGKITAYTIP
                     TLDEVLVWAKGKTILMLDIKEGVTQVMIMEALRRHEAFSYVVPITYNASEAASFIKLD
                     STLILSCSIEKEEDYHRLIAYGVKPRNIVAFCGISEPNKSLYQLLHAQNIFCQLGTMG
                     NLDRKAVARGDTVYYELLNNGADVLSTDRHVEAWSVIQHFLKDKKISDPYITFP"
BASE COUNT          599 a          467 c          387 g          583 t
ORIGIN      
        1 ggagattgca gccttagaaa aagagaaaaa cgaactggag aataaactca atagcggttc
       61 cgcggattac attgaacttc aaaaatggtc tgaacgttta tccgtcattt cgcaaacgtt
      121 ggatgaaaaa tcaatgcgtt ggttggaatt agccgaatac acatccgcca aataatcgca
      181 acatattctc gacgccggat gcagtgaaaa cgacgtattg aggtgcccgt cattacggct
      241 aacgtgataa acgtacggca tcaatattaa aaaattccgg aatttttccg gttttatctt
      301 tataaaaatc cctgatcttt gccatatccc ctatttcatc accggtcata tatagtgccg
      361 gtccaaagcc tgcgagcttc ttttcataat ccaaaaaacc caatacgatc ggtacattgg
      421 caccgagcgc cagatgataa aacccggact tccatttttc aacgcgctta cgggtacctt
      481 ccgcaggtat gatgatcatc aaatcttcac gcgccttgaa aacatcaatc atcgcatcca
      541 ccatactgtt ttttcccgag cgatccacag gaatgccgcc catacgcaaa aacatgcctt
      601 tcaacggaaa tcgaaagagt tcttttttcg caagaaaatt aacacgcata ttaaaacatt
      661 cgagcgctcc tataccatac acaaagtccc aattactggt atgcggcgca ccgatgatca
      721 cgcatttttt tatattcgga gcgataggtg ctgtgatcgt ccatcccttc attcggaaaa
      781 accagcggaa aagcttagcg atcatattgt ttattacatc aattagtatg aaaaattagg
      841 ttcggaattt aacgcgtatt atttttgaat agtgcgccga ggttttgaat acagcacagg
      901 cttcggatgt cggataattt taccttgcgc ttcgagttca cgggcaacag aaatcgtata
      961 ccacgataac gagccctcaa aatcgggaat cttcttctct acaagccgta ctatttccgt
     1021 aaatcgtata ggactagtgg tcaaaacgga taatatggct ttcgatactt ttttgtattt
     1081 gtccgaatca atggccacgg atcttttcca accgaggcct tttacacgca ctcgtttttt
     1141 ggactgtact gggcttttct ttggcataaa tgacattttc tctctgaata cgacatcaac
     1201 gtaagaaata ttaaaaatca tggaaatgtg atatacggat cggaaatttt tttatctttg
     1261 agaaaatgct gaatcactga ccacgcctca acatgacggt ccgtagataa cacatcggca
     1321 ccgttattta ataattcgta atataccgta tcaccgcggg ccacggcttt tctatcaagg
     1381 ttacccatag ttccaagttg gcaaaaaata ttttgcgcat gtaaaagctg atacaatgat
     1441 ttattcggtt ctgaaattcc acaaaacgct actatattcc ttggtttcac accatatgcg
     1501 atcaagcggt gataatcttc ttctttctca atactgcatg ataaaatcaa cgtagaatcc
     1561 agtttgataa acgacgccgc ttcggaagcg ttataagtta tgggaacgac atagctgaat
     1621 gcctcatgac gtcgaagcgc ttccatgatc attacctgcg tgacaccttc ttttatgtca
     1681 agcatcaata tagtttttcc cttagcccaa actaacacct cgtcaagcgt aggaatagta
     1741 tatgccgtga ttttaccgtc gttgtctttt aatcgcaatt gcttcagatc tgcaaaaagc
     1801 atatccgata cagggccttt gccggtagtc gtgcgatcta gtgttttgtc gtgcatcatg
     1861 accagattac catctttact tatacgcaca tcacactcaa tcaacgcata agtatacttg
     1921 atggtatttt caaatgttgc caaacagttc tcaggaaaac cggataccgg gccgccgcga
     1981 tgagcgctca cgagcggaga acggccttcc gaccacgtaa ggaatttttg taattc
//
LOCUS       DAOQGF010000250         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_37942_length_2027_cov_4.931034, whole genome shotgun sequence.
ACCESSION   DAOQGF010000250 DAOQGF010000000
VERSION     DAOQGF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37942_length_2027_cov_4.931034"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2027
                     /locus_tag="PK937_16450"
     CDS             <1..>2027
                     /locus_tag="PK937_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNH34216.1"
                     /translation="GILVRTMYSLISAGTERTTVETAQKSLVGKAKSRPDLVKKVLQV
                     AKRDGVLATLQLVKNKLDTPVPLGYSSAGYVVAVGEGVTEFAVGDRVACAGQGYASHS
                     EVVYVPINLAVKVPEQVPLDEAAYATLGAIALQGIRQSEVTVGDNVAVIGLGLLGLLT
                     VQMLKSAGCRVVGFDINPNACARAKSMGADYALSPADDMDAMLQKLTRGYGVDSAIIT
                     AATSGNEPIILAGDICRNKGNMVIVGAVPANVPRSPFYEKEIDVRFSRSYGPGRYDVK
                     YEEGGIDYPYGYVRWTENRNMQAFLDLIDAKKIDLQSVTTHRFKIEDAIQAYDLITGK
                     SKTKEPHIGLLLTYDHRTPDSSDTAFQIRESSEFPSLEGRVRIGFIGAGSFAQNYLLP
                     HLKKNKRVQLIAVATSKGVTSSGVAKKFKFREAASDDRSIATHTDIDTVFIATRHHQH
                     ADQVNQCLRKGKSVFVEKPLALNMEQLQSVIDTHNQTGGRVMVGFNRRFSPTIQEVRK
                     FFDKKVQPAMIHYRINAGFVPKEHWTQDAAQGGGRIIGEVCHFVDLIMYLTGSLPIKV
                     YADTISSNLDSLIAHDNASITFKFRDGSIGTIEYVAVGDKSFPKERIEMYCENSVAVI
                     DDFKKCTLTRNGKTKTFGGSKQEKGYKQCVESFIGSIQSGQDAPIPLRESV"
BASE COUNT          541 a          477 c          508 g          501 t
ORIGIN      
        1 ggcattcttg tacgcacgat gtattctctg atcagcgccg gcacggaacg tactaccgtc
       61 gaaacggctc aaaaaagttt agtaggtaaa gccaaaagtc ggccggatct tgtaaaaaaa
      121 gttcttcaag ttgcaaaacg ggacggcgtc ctggctacat tgcaattggt caaaaataaa
      181 ctggatacgc cggtgccgct cggttatagc tctgccggtt atgttgttgc tgtcggagag
      241 ggcgttaccg agtttgccgt cggtgatcgc gtggcatgcg caggacaagg ttatgcatcg
      301 cattcggaag tcgtatatgt accgatcaat ctggccgtaa aagtaccgga gcaggttccg
      361 ctggacgaag ccgcttacgc cacgctgggc gcgattgctc tgcagggtat tcgccaatcg
      421 gaagtcactg tcggtgacaa tgtcgcagtg attgggcttg gccttctggg acttctcacc
      481 gtgcagatgt taaaatccgc cggatgccgc gttgtcggtt ttgatatcaa ccccaatgcg
      541 tgcgcacggg ccaaatcaat gggcgccgat tatgctctgt caccagccga cgatatggat
      601 gctatgttgc aaaagcttac gcgcgggtat ggtgtggata gcgcgattat tacagccgcc
      661 acgtccggca atgaaccgat cattcttgcc ggagatattt gtcgtaataa agggaatatg
      721 gttatcgtcg gcgccgtacc ggctaatgta ccccgcagcc ccttttatga aaaagaaatt
      781 gatgtgcgat tttcgcggag ctacggcccc ggacgctatg atgtgaagta cgaagaaggc
      841 ggaatagatt atccgtatgg gtatgttcgc tggacagaaa accgaaacat gcaggctttt
      901 ttggatctga tcgatgccaa aaaaattgac ctccagtcgg ttactaccca tcgttttaaa
      961 atcgaagacg cgatacaggc ttacgacctt attaccggta aatccaagac caaagagccg
     1021 catatcggat tacttttgac gtatgatcat cgcacgccgg atagcagcga tacggcattt
     1081 caaatccggg aatcgagcga atttccatct cttgaaggac gcgttcgcat cggttttatc
     1141 ggtgcaggaa gttttgcgca aaattattta ttgccccatt tgaaaaaaaa caaacgcgtt
     1201 caacttatcg cagttgctac atccaaaggg gtcacctcgt cgggcgttgc taaaaaattc
     1261 aaattccgcg aagcagcctc cgatgatcgt tcgatagcaa cgcatacgga tattgatacc
     1321 gtttttatag ctacgcgcca tcaccaacat gcggatcaag tgaatcaatg tttacgtaaa
     1381 ggtaaatccg tttttgtcga gaaacctctg gcgctcaata tggaacagct tcagtccgtc
     1441 attgacacgc acaatcaaac cggcggacgc gtgatggtcg gatttaatcg tcgtttttct
     1501 ccgacgattc aagaagtaag aaaatttttt gataaaaaag tacaaccggc aatgatacat
     1561 tatcgcatca atgccggatt cgtacccaaa gaacactgga ctcaggatgc ggcgcaaggc
     1621 ggaggtcgca tcatcggcga agtctgccac tttgttgacc tgatcatgta tcttacgggt
     1681 tctctgccga tcaaagtgta tgcggacacg atctcaagca atctggattc gctgatcgct
     1741 catgataacg cttctatcac atttaagttt cgcgatggct cgatcggaac gatcgaatac
     1801 gtagccgtgg gcgacaaatc gttccccaaa gaacgtatcg aaatgtattg cgaaaactca
     1861 gtcgcggtga ttgatgattt caaaaagtgc actctgacgc gcaacggaaa aacgaaaact
     1921 tttggcggct ccaagcagga gaaaggttac aaacagtgtg tagaatcttt tatcggatca
     1981 attcagtccg gtcaagacgc tccgatcccc ttacgcgaat ccgtcaa
//
LOCUS       DAOQGF010000251         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_38153_length_2020_cov_6.106870, whole genome shotgun sequence.
ACCESSION   DAOQGF010000251 DAOQGF010000000
VERSION     DAOQGF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38153_length_2020_cov_6.106870"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PK937_16455"
     CDS             complement(<1..1824)
                     /locus_tag="PK937_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNH34217.1"
                     /translation="MKKRLLWIIAVMITVPVYAQTNVTLTPEYLYNGEIKQRTEAPPY
                     FWTSQNTIILYDTRVAWPDRSYQMLDPQSGERFKLFDMRTAIAGLQKHISSVPAALPW
                     PEGLDASGSLALFSFEGDVFIFHFKTSQWTRVTATSEEEMAPRLAPDGKKVVYVRHND
                     LFSYDLVSQKETQITSDGSSTLLNGRLSWVYWEEIYDREELGSFWSDDGSLLAFYKFD
                     ESQVDEVVFQDYKPAVPALIRQRYPKTGSVNPIATLHCTNMNTGKTVAVDMTGLTYEY
                     IVRVKWLPDNKRLAMQTMNRSQTELNLYFVDAASGRPTRILTEINSGFVREHNDLYFL
                     KGGSEFIWSSQRSGYTHLYHYQSNGRLIRPVTSGTWSVQMTTLYSKGGVVGIDEKTQT
                     LYYQSLEKSSIERHIYSIRFDGKNKKRLSIKDGTYDATLSPDGRFLMYHHSSANTPPS
                     LTLHKPDGSIKQVIAAADTTYARTLGFVPYRFFTIATRDSFPMPAMMLRPADFDSTKK
                     YPVILHVYGGPSAPQVNHRWPTETIMPHQLWVRDGYIIITVDNRSATGISKILENTVV
                     RMQASDGELRDLEDAVAWIKTRSFVDPERIGIWGRSGGGTFT"
BASE COUNT          504 a          467 c          496 g          553 t
ORIGIN      
        1 agtaaaggtt ccgccaccac tgcgacccca tatgccaata cgttcggggt caacaaatga
       61 tcgcgtttta atccaagcca cggcatcctc tagatcgcgt aattcaccgt cgctggcttg
      121 catgcggaca acggtatttt ccagtatttt gctgattccg gtagcgcttc gattatccac
      181 ggtgatgatg atatatccgt cgcgcaccca taactgatgc ggcatgatgg tctctgtcgg
      241 ccagcggtga ttgacttgcg gtgcgctcgg gccgccgtac acatggagaa tgacgggata
      301 ttttttggtc gaatcaaaat cggcaggacg cagcatcatt gcgggcatcg gaaaactgtc
      361 acgcgtggcg atcgtaaaaa aacggtatgg tacgaatccc aaagtacgcg catacgtcgt
      421 atccgccgcg gcgatcactt gtttgatgga gccgtccggt ttgtgcaatg taagggaagg
      481 cggtgtattg gcggatgaat gatggtacat tagaaaccgt ccgtccggac ttagtgtcgc
      541 atcatacgta ccatctttga tcgaaagtcg ttttttattt tttccgtcaa aacgaatgct
      601 gtaaatgtgg cgttcaatcg aagatttttc taatgactga taatataagg tctgcgtttt
      661 ttcatctata ccgaccacac cgcctttgct gtacagcgtt gtcatttgta cagaccatgt
      721 tccggacgta acggggcgta tcaagcgccc gttactttga taatgataca gatgcgtgta
      781 tccgctgcgt tgagacgacc agataaattc gctgccgcct tttaaaaaat acaaatcgtt
      841 gtgttcgcgg acaaatccgg agtttatttc tgtaagtatc cgtgtaggtc ttccggaagc
      901 cgcatctaca aaataaagat tcaattcggt ttgcgaacgg ttcatcgtct gcatggccaa
      961 tcgcttgttg tcgggaagcc attttacccg cacgatgtat tcatacgtca ggccggtcat
     1021 atccacagcc accgtttttc cggtattcat gtttgtgcag tgcagggtag cgataggatt
     1081 gacgcttccg gttttaggat aacgttggcg tatcaatgcc ggaacggcgg gtttataatc
     1141 ctgaaaaacc acttcgtcaa cttgcgattc atcaaattta taaaacgcaa gtagcgaacc
     1201 gtcgtctgac caaaagctgc caagctcttc gcgatcatag atctcttccc aataaaccca
     1261 ggacaaacga ccgtttagta atgtcgaact tccgtcggag gtgatctgtg tttctttttg
     1321 cgaaacaaga tcgtaactaa acaaatcatt gtggcggacg taaacgactt tttttccgtc
     1381 cggagcaagc cggggcgcca tttcttcttc cgatgtcgcg gttacgcgcg tccattgtga
     1441 tgttttaaag tgaaagataa atacgtcgcc ttcaaaagaa aataaagcca gcgaccctga
     1501 tgcatccaaa ccctccggcc aaggcagtgc ggcgggaacg gaggagatat gtttttgtaa
     1561 acccgcgata gccgtacgca tatcaaagag tttaaagcgt tccccgcttt gggggtccag
     1621 catttgataa gaacggtcgg gccatgcgac gcgtgtgtcg taaagtataa tggtattctg
     1681 cgaagtccaa aaataaggag gcgcttccgt gcgttgttta atttcaccgt tgtacagata
     1741 ttcaggcgtg agtgttacgt tcgtttgagc gtaaacgggc acggttatca tcaccgctat
     1801 tatccataat aatctttttt tcatacatca ctttgccggt attatgggtt gttatgcatt
     1861 gttgggattc attcgaagct ggattgcgtg caattttttt ccggtacgat acccgtgaat
     1921 tcccatagcc ggtccgatca gccaatacgt taggccggcc caattggcca tagcggagcc
     1981 gccgtataaa agtaaagagg ttgaaaaaag cgcaataccg
//
LOCUS       DAOQGF010000252         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_38222_length_2018_cov_3.768721, whole genome shotgun sequence.
ACCESSION   DAOQGF010000252 DAOQGF010000000
VERSION     DAOQGF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38222_length_2018_cov_3.768721"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PK937_16460"
     CDS             complement(<1..624)
                     /locus_tag="PK937_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH34218.1"
                     /translation="MKKSGTHLKKIFTTAAWIIVALTAVAQAQDLPTPASSQKQPILL
                     RNGRVHVGDGRVLEKTDILLDNGKIVEIGPGLSKPSTAREVDATGKEIYPGLIACYST
                     LGLSEIEAARPTRDDREVGSINPNARALIAFNTDSRVIPTVRSNGVLLAQITPQGGAL
                     SGTASVVQLDAWNYEDAVVSADNGLIVQWPSMSISSSIFAPPADVQKK"
     gene            complement(634..>2018)
                     /locus_tag="PK937_16465"
     CDS             complement(634..>2018)
                     /locus_tag="PK937_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNH34219.1"
                     /translation="DSSVLSPISFPNMAFGLKEIPVQQTVLIRNATVWTNTSKGILKE
                     TDVLFEKGKIKQVGKNLSAPAGAWIVDGTGKHVTTGIIDEHSHIAVSRGVNEGTQSVS
                     SEVRIGDALDAEDSDIYRQLAGGVTSSHLLHGSANAIGGQTQLIKLRWGAAAEELKFP
                     NWPGFIKFALGENVKQSNWGDDFTTRYPQTRMGVEQIIRDAFQAAKEYKSTWESYQKS
                     GGEKSGKVAPRRDLELDALVEILDGKRHITCHSYVQSEITMLMRVAEEMGFRINTFTH
                     ILEGYKVADKMKKHGAAGSTFADWWAYKYEVIDAIPHNPGLMHQEGVTVGINSDDAEM
                     ARRLNQEAAKSVKYAGVSEEDAWKFVTLNPAKMLRVDDRVGSLDIGKDADIVVWNNNP
                     LSIYAVPEKTFVDGRLYFDKEADARLQIEMNQERSRLIQKMIADKKGGAPSVKPIPQK
                     KIRYHCVGEE"
BASE COUNT          468 a          545 c          451 g          554 t
ORIGIN      
        1 tttcttttga acatcggccg gcggcgcaaa tatactgctg gaaattgaca tagacggcca
       61 ctgcacgatc aaaccattgt ccgcagaaac cactgcgtct tcatagttcc atgcatccag
      121 ctgaacgacg gaagctgttc ccgacaaagc gcctccttgc ggtgtgattt gtgccagaag
      181 cacgccattg gaacgcacgg taggaataac acgggaatcg gtgttaaatg ctatcaaagc
      241 gcgcgcattg gggttgatcg aaccgacttc ccgatcatca cgcgtcggac gtgccgcttc
      301 gatctccgac aaaccgagcg tgctatagca ggcaatcaat ccgggataaa tttcttttcc
      361 cgttgcgtcc acctcacgtg cggtggaagg cttgctaagc ccaggaccaa tttcgacgat
      421 tttaccgtta tccagaagga tatccgtttt ttccagcacg cgcccgtcac cgacatgtac
      481 gcggccattg cgtaacagaa tcggctgctt ttgggatgat gcaggcgtcg gcaaatcctg
      541 ggcctgtgcg acggcggtaa gcgccactat gatccatgcg gccgttgtaa atattttttt
      601 caaatgcgtt cctgattttt tcatattttt ctcctattcc tcaccgacac agtggtaacg
      661 aattttcttc tgcggtatgg gttttacaga cggggcgccg ccttttttat ccgcgatcat
      721 tttttgtatt aaccggctgc gttcttgatt catttcgatt tgcaaacggg catccgcttc
      781 tttatcaaaa tataaacgcc cgtccacgaa agttttttcg ggcacggcat aaatcgaaag
      841 cgggttatta ttccatacta cgatatccgc atctttgcca atatccaggc tgcccacacg
      901 atcatccacg cggagcattt ttgccggatt gagcgttaca aatttccacg catcttcttc
      961 tgaaacgccg gcatatttta ccgatttggc cgcttcttga ttgagtcgcc gcgccatttc
     1021 ggcgtcatcg gaattgatac ccacggtaac accttcctgg tgcatgagcc caggattatg
     1081 cggtatcgca tcaatcacct catatttgta tgcccaccaa tcggcaaacg tagaacccgc
     1141 tgcgccatgt tttttcatct tatcggctac tttgtaacct tcgagaatat gcgtaaatgt
     1201 attgatgcga aaacccattt cttcggcgac gcgcatcagc atcgtaattt ccgattggac
     1261 gtaagaatgg catgtgatat gacgtttccc gtcgagaatt tcgaccaggg catcaagctc
     1321 cagatcgcgg cgcggagcaa cttttccgga tttttctccg ccggatttct gataactttc
     1381 ccacgtcgat ttgtattctt tggccgcctg aaacgcatca cggatgattt gctcaacgcc
     1441 catgcgggtt tgcggatacc gcgtcgtaaa atcatcaccc caattggact gtttgacatt
     1501 ttcaccgaga gcaaacttaa taaaaccggg ccagtttgga aattttaatt cttcagccgc
     1561 ggcaccccag cgcaatttga tcagttgtgt ctgcccgccg atcgcattag cagagccgtg
     1621 caaaaggtgc gacgatgtga ccccgccggc caattggcga tatatatccg aatcctcggc
     1681 atccaaggca tcgccgatgc gcacttccga actcaccgat tgcgtacctt cattgacacc
     1741 acggctcacc gcgatgtgcg agtgttcatc aataataccc gtcgtcacat gtttgcccgt
     1801 cccatcaacg atccatgcac ccgctggcgc cgaaagattt tttccaactt gtttgatttt
     1861 acctttttca aacaacacat cggtttcttt taaaatccct ttcgacgtgt tagtccacac
     1921 cgtagcgttt cggattaata ccgtttgttg tacgggaatt tctttcaaac cgaaagccat
     1981 attgggaaaa ctgatcggtg ataatacgga cgaatccg
//
LOCUS       DAOQGF010000253         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_41075_length_1935_cov_9.642021, whole genome shotgun sequence.
ACCESSION   DAOQGF010000253 DAOQGF010000000
VERSION     DAOQGF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41075_length_1935_cov_9.642021"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PK937_16470"
     CDS             <1..1195
                     /locus_tag="PK937_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNH34220.1"
                     /translation="TSIASGIYPALYISRFHAAGILKGSVRFGKNNPLTKVLLSIQLV
                     LACMFMTCAVDFTRNTDFLSNRSWGYENREVLYTAVPDRSAFEQLRSKLLQEPNALSI
                     SGSTHHLGKKHVTTLIQRPERAYEVDQLFVDAHYFQTMGIPISRGRYFPDEAQADQRS
                     IVINETMVKHLNLENPIGHKLRIDSTEYSVIGVTQDIHVYDFSSAIRPTLFRVASPDI
                     TCFLSVKVRPGTQPEMYRILKNHWSSLFPEIPFDGGYQEDVWGRYYETIGIHGLVWRI
                     FAFFAVAMAGLGLYGLITYNVTGRIKEFSIRKVLGAEAFSISALLSRPYTLLYGAALL
                     VGMPLSYLFATHLLVASYPYHMPVSYTGVFIAALALVLVLALSLSIQIHRILKSNPVE
                     GLRTE"
     gene            1265..>1935
                     /locus_tag="PK937_16475"
     CDS             1265..>1935
                     /locus_tag="PK937_16475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNH34221.1"
                     /translation="MNFKVTSSWLYLFVLLLLSGITVVTAQSDIERGRELFKNKRYSE
                     AKPIFEGILNGNEKNAEANYYLGMIYWRYVGDLDKASELLETATENDESNAQYHFLYS
                     GVLGVRAMRSNPIKQAYLATKIRNQLEKTIELEPTHVDARYNLIQFYIMAPGFMGGSI
                     EKAKVQAGALVKYNAARGYIAYAQIATNEKDDASAENYFFKAIAADPSLPGAYHQLGY
                     LYVKL"
BASE COUNT          509 a          431 c          448 g          547 t
ORIGIN      
        1 cacgagtatc gcatcgggca tttatcctgc gttgtatatt tcacggtttc atgccgccgg
       61 tattctgaaa ggctctgtgc ggtttggtaa aaataatcct ctcaccaaag tactcctgtc
      121 catccagttg gtactggcat gcatgtttat gacctgtgcc gttgatttca cgcggaatac
      181 tgattttttg tcgaaccgca gttggggata tgaaaatcgt gaagtattat acactgccgt
      241 tccagaccga tccgcatttg aacaattacg gtctaagtta ttacaagaac ctaatgcact
      301 ctcgatttcg ggctcgacac accacctcgg caaaaaacac gtgactacgc ttattcagag
      361 accggaacga gcttatgagg tggatcaatt atttgttgat gcgcattatt ttcagaccat
      421 gggaattccc atcagcagag gccgatattt tcctgacgaa gcccaagccg atcagcgcag
      481 tatcgtgatc aatgagacca tggtgaagca cttaaattta gaaaatccca tcggccataa
      541 attacgtatt gatagtacgg agtatagcgt gatcggcgta acacaggaca tccacgtgta
      601 cgacttttct tcggcaatac ggccgacatt atttcgcgtt gcctcaccgg acattacgtg
      661 ttttctgtcc gttaaagtgc ggccgggtac acagcctgag atgtatcgca ttttaaaaaa
      721 ccattggtca tccctgtttc ctgaaatacc ctttgatggc ggttatcagg aagatgtttg
      781 gggccggtat tatgaaacga tcggaattca tggattggtt tggcgcatat ttgctttttt
      841 tgcagtggct atggcaggac tcggtctgta tggccttatc acctataatg ttacgggacg
      901 tatcaaagag ttcagcattc gcaaagtact tggcgctgaa gccttctcga tatcggctct
      961 tctttcgcgt ccttatacgc tgctttacgg tgcagcgcta ttagttggca tgccattgag
     1021 ttatttgttt gcaacgcatt tgcttgtcgc ttcatacccg tatcacatgc ccgtcagcta
     1081 taccggagtt ttcattgctg cgcttgcact ggttcttgtt ctggcgttgt cgctttcgat
     1141 tcagatacac cgtattctga aatcgaatcc cgtagaagga ttgagaacgg aataaaacat
     1201 ttacatgcgg ctttcgtaag atattcaacg aataattacg aataatcatc gaaaggaaat
     1261 cgtcgtgaac ttcaaagtta ccagctcttg gttgtatttg tttgtactcc tgctgctctc
     1321 cggaatcact gtggttactg cacaatcgga tatcgaacgg ggtcgcgaat tatttaaaaa
     1381 taagcggtat agcgaagcca aacccatatt cgaaggaatt ctcaatggga atgaaaaaaa
     1441 cgctgaggcc aactattact tgggtatgat ttattggcgc tatgtcggtg atcttgacaa
     1501 agcgagtgaa cttctcgaga cggccacgga aaacgacgag tccaacgcgc aataccactt
     1561 tttatattcc ggcgttctgg gagtcagagc catgcgcagt aaccccatta agcaagccta
     1621 tctggcgaca aaaatccgca atcaattgga aaaaacaatc gaacttgaac cgacacacgt
     1681 ggatgcgcgt tataatctaa ttcaatttta catcatggcg cccggattta tgggtggtag
     1741 tatcgaaaaa gctaaggtgc aagcgggagc gctggtcaag tataatgctg cgcgggggta
     1801 catagcgtat gcgcagatcg caaccaatga aaaagacgat gcgtctgccg aaaattattt
     1861 tttcaaagct atagcggccg atccgtcgct tcccggcgct tatcatcagt tgggttatct
     1921 ttacgtgaaa ctaaa
//
LOCUS       DAOQGF010000254         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_41757_length_1917_cov_4.289474, whole genome shotgun sequence.
ACCESSION   DAOQGF010000254 DAOQGF010000000
VERSION     DAOQGF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41757_length_1917_cov_4.289474"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PK937_16480"
     CDS             complement(<1..388)
                     /locus_tag="PK937_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNH34222.1"
                     /translation="MKSYTTSLEIILYVKDQTKSRDFYRNILQREAVLDVPGMTEFLL
                     TDTCKLGLMPTHGISKIIGDRMPHPDTGVGIPRCELYLFVENAHEQLENALRHGAAFI
                     DPIAERDWGDTVGYVADPDGHVIAFAS"
     gene            complement(427..765)
                     /locus_tag="PK937_16485"
     CDS             complement(427..765)
                     /locus_tag="PK937_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNH34223.1"
                     /translation="MRNRKKRLRVRDVQDVLDVIGGRWRGPILASLCEKQKRFNELQR
                     DLHPITPRILTRELKYLEMNKLVFRSANGKTSSVVVYGLTPHGQSLDPVIDGIVAWAQ
                     KHRKEVLGYE"
     gene            861..1493
                     /locus_tag="PK937_16490"
     CDS             861..1493
                     /locus_tag="PK937_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNH34224.1"
                     /translation="MKISVIGASSGVGLQCVQRALQRGHAVTTLSRSPLPVNPSNDLL
                     IIRGSASNKEDIKKTIKDADAVLVSIGTGKSVKATTLYSDAAQVLVEARDELGTDIPF
                     IVLTGFGAGNSGQFHTGFMKFVFRFILQRVYENKTRMETIIAASRMRWVFVRPGVLTD
                     KEISEKYRVETRYYKGMRIGSIARSDVADFMVKQAENPSAIGQYAALSNK"
     gene            1677..>1917
                     /locus_tag="PK937_16495"
     CDS             1677..>1917
                     /locus_tag="PK937_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNH34225.1"
                     /translation="MSKIRVESFTISLDGFGAGIDQTMDNPLGVGGQNLHQWAMPTKT
                     FQKALFGSDNGTTGIDDKFATRGFDNIGAWILGRNM"
BASE COUNT          545 a          404 c          433 g          535 t
ORIGIN      
        1 tggatgcaaa tgcgataacg tgaccatccg gatcagctac atatcctacg gtgtctcccc
       61 aatctctttc agcgatgggg tcaataaacg cagcgccatg tcgtaaagca ttctcaagtt
      121 gctcgtgagc attttccaca aaaagataaa gttcgcaacg cggtattccc actccggtat
      181 caggatgcgg catacgatct ccgatgattt tggaaatgcc gtgcgtgggc attaacccaa
      241 gtttgcatgt atccgtgagc aaaaactcgg tcatgcccgg tacatcaagc acagcttcgc
      301 gttgaaggat attacggtaa aaatcgcgac ttttcgtctg atctttgaca tacagaataa
      361 tctcaagtga cgttgtataa gatttcatac tttatgtttt aataatatgt aattttgcga
      421 agtaatttat tcgtacccaa gaacctcttt acgatgtttt tgagcccagg ccacgatgcc
      481 gtcaatgacc gggtcaagtg attggccatg aggagttaac ccataaacaa cgacggaaga
      541 cgttttacca tttgcagatc taaatactaa tttattcatc tcaagatatt tcagttcgcg
      601 cgtcaatata cgcggcgtga tcggatggag atcacgttga agttcgttaa accttttttg
      661 cttttcgcat aagctggcta aaatagggcc acgccatcgt ccgccgatca catccaaaac
      721 atcctgtaca tcccttacac gcaaacgttt ctttctattt cgcatagtaa tatcctaaag
      781 gatagcattt tttccggttg gactcttgtc caaagtaact aacgtgatta ttttttgaaa
      841 ggaaataaaa aggagcaaac atgaaaatat cggttatcgg cgcatcttcc ggggtcggat
      901 tacaatgcgt gcagcgggcg cttcagcgag gtcacgccgt gactacgctc tcccgctcac
      961 cgttacccgt aaatccatcg aatgatcttt tgattatacg cggaagtgct tcaaacaaag
     1021 aggatattaa aaaaacaata aaagacgcag atgccgtact tgtatctatc ggtacaggaa
     1081 aaagcgtaaa ggcgactaca ctttattcgg atgccgccca agttttagtt gaagcaagag
     1141 acgaacttgg aacggatata ccgtttatcg ttttgacggg atttggtgca ggtaacagcg
     1201 ggcagtttca taccggattt atgaaattcg ttttccgttt tatacttcaa cgtgtgtatg
     1261 aaaacaaaac gcgtatggag acgatcatcg cagccagtcg catgcgatgg gtttttgttc
     1321 gccccggtgt tttgacggat aaagaaattt cagagaagta tcgtgtcgaa actcgatact
     1381 acaaaggtat gcgtatcgga tccatcgcgc ggagtgatgt agccgatttt atggtaaagc
     1441 aagctgaaaa tccctccgct atcggtcaat acgccgctct ttcaaataaa taatccttac
     1501 attccagcct ccggttttcc ggcgtgacga tcaaggaata tgttttataa taactgtatc
     1561 agaccacgat ttgttttgtg acgggaaaag agatttgttt ttttgggcga aatgagtaga
     1621 ataccggagc aatgcggttt tgaactttta ctttacaaaa tactccggag aacgccatgt
     1681 ccaaaatacg tgtagagagt ttcactattt cactggacgg attcggtgcc ggcattgatc
     1741 agacgatgga taatcctctc ggtgtaggcg gacaaaatct acatcagtgg gcgatgccga
     1801 caaaaacatt tcaaaaagcg ctgtttggtt ccgataacgg tacgacgggt atagatgaca
     1861 agtttgcaac acgaggtttt gataatatcg gtgcgtggat actgggtcga aatatgt
//
LOCUS       DAOQGF010000255         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_42155_length_1906_cov_7.584549, whole genome shotgun sequence.
ACCESSION   DAOQGF010000255 DAOQGF010000000
VERSION     DAOQGF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42155_length_1906_cov_7.584549"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PK937_16500"
     CDS             complement(<1..965)
                     /locus_tag="PK937_16500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNH34226.1"
                     /translation="MLKTIPHLLISDAQGRFSDIPQIAMAGRSGKNTVPVAADTLIPL
                     PEGSQLYFLPQRKAMGYDRKTGELIVFDDAYAVAAYIPPSYTHYLMAAYETITGAPRL
                     PLFSFTAVGYYRGRYYVPAVHVDEDIKQLPTSFDDKKVKHAVDAWIVRHPNNRLLAHH
                     GHACAIEYGCPNAKNLFLGRWEAPVAVASACNANCVGCISFQPKESVPSPQNRLDFVP
                     TVEEIVEYAVPHLEKAEHAILSFGQGCEGEPLLRGKLIEDSIRAIRRHTKRGVIHINT
                     NGSKPDTLARLFDAGLDSVRVSMNSAQLDLYHRYYKPNNYTFDDVV"
     gene            complement(987..1484)
                     /locus_tag="PK937_16505"
     CDS             complement(987..1484)
                     /locus_tag="PK937_16505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNH34227.1"
                     /translation="MSQPLTKPVLLIVDDEVEILKVLQKSLEDDYDVLTASRAKAGLE
                     LLDERVSVVISDQRMPEMTGSEFLRHVRERRPEVVRIIMSGYSDMTALINSVNYGEIF
                     RYLNKPWDLNELLNTIELAVKRYDENRRQIELASENQRLAAENLRLTEDLREKTTEIN
                     RLRGK"
     gene            1704..>1906
                     /locus_tag="PK937_16510"
     CDS             1704..>1906
                     /locus_tag="PK937_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34228.1"
                     /translation="MAHDAQAGSSTTGSHHFTPYIAPEHNVPEFTAKALILGALFGII
                     FGAATVYLALKAGLTVSASIPIAV"
BASE COUNT          500 a          449 c          446 g          511 t
ORIGIN      
        1 actacatcgt caaacgtata gttgttgggt ttgtaatagc gatgataaag atccagctga
       61 gccgagttca tgctcacgcg gacactgtcg aggcccgcat caaacaaccg cgccaaagta
      121 tccggtttgc tgccgttggt gttgatatga ataacaccac gcttggtgtg tcgacgtatt
      181 gcccgtatag aatcttcgat cagtttaccg cgtaacagcg gttcgccttc gcatccttga
      241 ccaaaactca aaatggcatg ttcggccttt tccaagtgcg gtacggcata ttcgacgatt
      301 tcttcgaccg taggaacaaa gtctaagcga ttttgcggtg atggaacaga ttctttcggt
      361 tgaaatgaaa tgcaaccgac acaattggca ttgcacgccg aggctacagc gaccggggcc
      421 tcccaacgtc cgagaaaaag atttttagcg ttaggacaac cgtattcgat agcacaggca
      481 tggccatgat gcgccaataa acgattatta ggatgacgaa cgatccacgc atcgacagcg
      541 tgcttcactt ttttatcatc aaaagacgtc ggcaactgtt tgatatcttc atcgacatgc
      601 acggcaggta catagtaacg accacgataa taacccactg cggtaaaaga aaaaagcgga
      661 agccggggtg ctcccgtgat ggtttcgtag gccgccatga ggtaatgcgt atatgacggc
      721 gggatgtatg ctgcgacagc atacgcatca tcaaatacta tgagttcgcc ggtttttcta
      781 tcgtacccca tagccttacg ttggggtaag aaatataatt gcgaaccttc cggcagaggg
      841 atcaatgtat cggctgcaac aggaaccgtg ttttttccac tacgcccggc catggcaatc
      901 tgcggaatgt cgctaaatcg cccttgcgca tcgctgatca gcagatgagg tatggtttta
      961 agcatgatag aatggaattc ggggaattat tttccgcgca ggcggttaat ttcggttgtc
     1021 ttttcgcgaa ggtcttcggt aaggcggagg ttttcagcgg ctaggcgctg attttcagag
     1081 gctaattcta tctgacggcg attttcgtca taccgtttga cagctaactc tatcgtattg
     1141 agtaattcgt tgagatccca aggcttattg agatatctga aaatctcacc gtagtttacg
     1201 gaattaatca aagccgtcat atcagagtac ccggacatga taatacgaac cacttcgggg
     1261 cggcgttcgc ggacatgacg taagaattct gaaccggtca tttccggcat acgttgatcc
     1321 gaaataacga cggatacccg ttcgtccaaa agctccagac cggctttggc acgcgatgcc
     1381 gtcaagacgt cataatcgtc ttcaagtgat ttttgcaaaa cctttaaaat ttcgacttca
     1441 tcatctacga tgagcaaaac cggttttgtt agcggctggg acatgccgtt tcctgtcata
     1501 atgagaaata ggtagtataa aaaccgtttt aaagatacaa tcaaagcatc gcaaaatcaa
     1561 aatattttct ctaaaaacgg tttgacttta ttgggatgcc gacctatatt ttttccggat
     1621 ttttcagcct gtcgctggca cttccatata ccattcaaaa tactttctat acatcttcgt
     1681 tatactaacg gaaaaggtaa tctatggcac atgacgctca agcgggctct tcgacgacgg
     1741 gttcgcatca cttcacgcct tatatagcgc cggaacacaa tgtaccggag tttacggcca
     1801 aagcgctgat cctcggtgcg cttttcggta tcatttttgg cgcggctacg gtctatcttg
     1861 cgctcaaagc aggactcacc gtatcagctt ccattcctat cgctgt
//
LOCUS       DAOQGF010000256         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_42785_length_1890_cov_4.723706, whole genome shotgun sequence.
ACCESSION   DAOQGF010000256 DAOQGF010000000
VERSION     DAOQGF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42785_length_1890_cov_4.723706"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK937_16515"
     CDS             <1..363
                     /locus_tag="PK937_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34229.1"
                     /translation="EATTRLVTVAQETSEHTISSADMAQQTLTNLKEILEWAQRSSDA
                     AKQIYISINQQRLANRQISFSFNDISADMRALAASSERYAETVDALKKFSNNMEAVVK
                     YFVKQQHSEKPDHEKNTT"
     gene            344..1849
                     /locus_tag="PK937_16520"
     CDS             344..1849
                     /locus_tag="PK937_16520"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNH34230.1"
                     /translation="MKKTLREILNNNALVRNVYGSTSLTVTGLCTDSRFAQKDNIFYA
                     LRGTQKDGNKFIAHAIQKGASVIVSDELYAKVQFPPKTRVTYVQVENLTQFMAESSAS
                     FYGTTNAPMAFIGITSTNGKTTTAKFINETLKALGHKTVFLGTTGFEVCGRIIPTDFT
                     TPPAPMLHKYLREGMDKGATHVVMEVSSHALKLKRVWGLRFDAAVFTNLTHEHRELHV
                     SMDDYFSTKALLFGMLKPVGFAVINGDDDFSGGMRKSVPSGHRIVEYGKKNSATLRLE
                     KIKFNPDSRLQSIAFVQDGKSTAIETRMIGEFNAYNALAAYGVLEGLGLDMAVGAEKF
                     PTLPTVDGRFNWYKAGTFNVIIDFAHTPDGLEKLLQEVNRIKPQGRRLITVFGCPGSR
                     DPSKRPMMGKIATAASEHTIITTDDIHHESPEAIIQDITRELSETRHEIIIDRREAIR
                     RSLQIAQEGDFVVIAGRGHERYQYVGDEKIPFYDRQVFMEEAQKLELKVEE"
BASE COUNT          561 a          465 c          424 g          440 t
ORIGIN      
        1 gaggccacaa cgcgcttagt taccgttgcc caagaaactt ccgagcatac gatcagcagc
       61 gccgacatgg cccaacaaac actgacgaac ctcaaagaaa ttttagaatg ggcgcaacgc
      121 tcctccgatg cggccaaaca aatatacatc tcgatcaatc aacaacgttt ggccaatcgg
      181 cagatatcct tttcattcaa cgatatttct gccgacatgc gcgcattagc ggcctccagc
      241 gaacgttacg cggaaaccgt agatgcactc aaaaaattct ccaacaatat ggaagcggtt
      301 gtaaaatact tcgtcaaaca acaacattct gaaaaaccgg atcatgaaaa aaacactacg
      361 tgagattctt aataataacg ctttagtacg caatgtttat ggcagcacga gcctcactgt
      421 taccggactt tgtaccgatt cacgttttgc acaaaaagat aacatttttt acgccctccg
      481 tggtacgcaa aaagacggta acaaattcat agcgcacgcc atacaaaaag gcgcgtccgt
      541 catcgtctcg gacgaattat acgccaaagt acaattccct cccaaaaccc gcgtgactta
      601 cgtacaggtg gaaaacctga ctcagtttat ggcggagtcc tccgcttctt tttacggcac
      661 gaccaatgcg cctatggctt ttatcggtat tacaagcacc aacggaaaaa cgacaacggc
      721 caaatttatt aatgaaaccc ttaaagcatt gggccacaaa accgtgtttc taggtacgac
      781 ggggtttgaa gtgtgcggcc gaatcatacc aacggatttt acaacgccgc ctgccccgat
      841 gctccacaaa tatctacgcg aaggcatgga taaaggcgct acgcatgttg tcatggaagt
      901 ttcttcgcac gcactcaagc tcaaacgtgt atggggcctt cgttttgatg cggcggtatt
      961 taccaatctg acgcacgaac atcgtgaact gcacgtttcg atggatgatt atttttcgac
     1021 caaggcttta ctctttggca tgctcaaacc ggtcggtttt gccgtgatca acggagatga
     1081 tgattttagc ggaggtatgc gcaaatcggt tccctccggt catcgcatcg tagaatatgg
     1141 caaaaaaaac agtgccactt taagactgga aaaaataaaa tttaatcccg attcacgcct
     1201 tcaaagcatc gcctttgttc aagacgggaa atccaccgct atagagacac gcatgatcgg
     1261 agaatttaac gcatacaacg cattggccgc ttacggtgta cttgaaggac tcggcctaga
     1321 catggccgtc ggtgctgaaa aatttcccac gctaccgacg gtggacggac gttttaattg
     1381 gtacaaagcc ggtacgttta atgttatcat tgattttgcc cacacgccgg acgggttaga
     1441 aaaacttttg caagaggtga accggataaa gcctcagggg cggcgcctca ttacggtatt
     1501 cggttgtccc ggcagccgcg atccttcaaa acgaccgatg atgggaaaga tagctacagc
     1561 cgcgtccgaa cacaccatca tcacgacgga cgatattcat cacgaatcgc cggaagccat
     1621 catccaggat atcacgcgtg aattatccga aacgcggcac gaaatcatca tagatcggcg
     1681 cgaagctatt cgccgaagtc ttcagattgc acaagagggt gattttgtag tgatcgcagg
     1741 ccgcggtcat gagcgctacc aatatgtagg ggatgaaaaa attccgtttt atgatcggca
     1801 agtttttatg gaagaggcac aaaaattaga attaaaagta gaagaataaa aaaaagcccg
     1861 atgcgaatgc accgggcttt tttattacta
//
LOCUS       DAOQGF010000257         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_42865_length_1888_cov_4.331697, whole genome shotgun sequence.
ACCESSION   DAOQGF010000257 DAOQGF010000000
VERSION     DAOQGF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42865_length_1888_cov_4.331697"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PK937_16525"
     CDS             complement(<1..756)
                     /locus_tag="PK937_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNH34231.1"
                     /translation="MSDLTVTPHSDAPLNDAADLYRQISAALDTTVIGQSDIKREMII
                     ALLSGGHILMEGVPGIAKTLLAKTLAKALSCEFKRIQFTPDLMPSDVIGTNVFDPKSN
                     QFNVKRGPIFTHIALIDEINRAPAKTQSALLEVMEERQITIDGTRYLLPEPFMVMATQ
                     NPIEYEGTYPLPEAQLDRFMFKLIVPYPSASEEEQVLRAYHGGFDAHQIDQIKIEKPC
                     TPEMIARARHLIRQIVVDDGILKYITEVVRKTRE"
     gene            complement(766..>1888)
                     /locus_tag="PK937_16530"
     CDS             complement(766..>1888)
                     /locus_tag="PK937_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNH34232.1"
                     /translation="QPGGTKIFYKLLTDNGYNIERWRKKINLVPVAQEAQSIIMIAPS
                     DILSSQETDFLISFIHQGHTAFLFYSPALDDLLQKLHVRCNTDSSMISFPSGYLGNTV
                     SGDTLHPHIITSDSTTHTRFDDECVYFNSDSSAIIPLYTTLNGHTALEIRHGQGKLII
                     FNSPNYVTNAGLQLRHNADIIFRILQVDPGGNQREPGIIYWDEYHHGYKDYESLIAVL
                     DTWPIKWGMILSTISFLLWVHARAKRWGRPVPVITSSRRASIDYVHSVSGVYQNSSAH
                     TLALHHWYRWVMADLQRRYATRDPDKLALILHRRFALDIQKTKKLFAWIERKTIVVKS
                     PDLKNDPVQKREVSIDDEELIAGAKQLDHIYFLHYPTQS"
BASE COUNT          487 a          409 c          465 g          527 t
ORIGIN      
        1 ttcgcgcgtt ttgcgaacaa cctctgtgat gtattttagt attccgtcat ccactacgat
       61 ttgtcgaatc aaatgacgtg cccgtgcgat catttccggt gtacacggtt tttctatttt
      121 tatttggtct atctgatgcg catcgaagcc gccgtggtat gcgcgaagca cttgctcttc
      181 ctcactggcg gagggatacg gcacaataag cttaaacata aaacgatcaa gctgcgcctc
      241 aggcaaagga taggtccctt cgtattcgat aggattttgt gtagccatca ccataaaagg
      301 ttccggtaaa agataacgcg tgccgtcaat cgtaatttgt cgctcttcca ttacttccag
      361 taatgccgat tgcgttttag ccggcgcgcg attgatttca tcaatcagcg caatgtgggt
      421 aaaaatagga ccgcgtttga cattaaactg attggatttt ggatcaaaaa cattggttcc
      481 gatcacgtcg gacggcatca gatcaggcgt aaactgaata cgcttgaatt cacaagacaa
      541 cgctttggct aacgttttgg ccagtagcgt tttggctata ccgggtacac cttccatcaa
      601 gatatgaccg ccgctcagca gtgcgatgat catttcgcgt ttgatatccg actggcctat
      661 gacggtagta tcaagagcgg ccgaaatctg ccgatacaaa tcggctgcgt cattgagcgg
      721 tgcatcgcta tggggagtta ctgtcaaatc ggacatggta tatccttaag attgggttgg
      781 ataatgtaaa aaatagatgt gatccaattg ttttgcaccg gcgatcaatt cttcatcgtc
      841 aatgcttacc tctcgttttt gtacgggatc gtttttgagg tcgggcgatt tgacgacgat
      901 cgttttacgt tcgatccagg caaaaagttt ttttgttttt tgaatatcca gtgcaaaccg
      961 tcggtggaga atgagcgcga gtttgtccgg atcgcgtgtg gcatagcgcc tttgcagatc
     1021 ggccataacc catcggtacc aatgatgaag tgcgagcgta tgcgcggacg aattttgata
     1081 tacaccggac acggagtgca cgtaatctat cgaagcgcgt cgtgacgaag tgataaccgg
     1141 tacggggcga ccccaacgtt tggcgcgtgc gtgcacccac aacaaaaaac tgatcgtact
     1201 gaggatcatg ccccacttaa tcggccaggt gtccagaacc gcgatgaggg attcgtagtc
     1261 tttatatccg tgatgatatt catcccaata aatgataccc ggttcacgtt ggtttccgcc
     1321 ggggtctact tgcaagattc taaaaataat atcagcatta tgccgcagtt gcaaaccggc
     1381 gttagttaca taattcggtg aattgaaaat aattagcttt ccttgtccat gacgtatttc
     1441 aagagccgta tggccgttta atgtggtata aagcggaatg atggccgacg aatctgaatt
     1501 aaaatagaca cactcgtcgt caaaacgagt gtgcgtcgtc gaatcggagg ttattatgtg
     1561 aggatgcaat gtgtcgccgc taacggtatt tcccagataa cccgacggaa aagaaatcat
     1621 ggacgaatcg gtattacaac ggacgtgcag cttttggagt agatcatcca aagccgggct
     1681 ataaaaaaga aacgcggtat ggccttgatg aataaacgat atcaaaaaat ccgtttcctg
     1741 cgacgaaagg atgtcactcg gtgcgatcat tattatggat tgtgcttcct gtgctacagg
     1801 tactaggttt attttttttc gccaacgctc gatgttgtag ccgttgtcgg taagaagctt
     1861 gtaaaatatt tttgtgccgc ccggttga
//
LOCUS       DAOQGF010000258         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_43964_length_1861_cov_6.325581, whole genome shotgun sequence.
ACCESSION   DAOQGF010000258 DAOQGF010000000
VERSION     DAOQGF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43964_length_1861_cov_6.325581"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK937_16535"
     CDS             complement(<1..913)
                     /locus_tag="PK937_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34233.1"
                     /translation="MPQVRRYYLMITIFLLFGYTLKAQGKRPITADDLWAFKRLSGFT
                     VSPDNKYGCFVVTDYNVKENKGNSDLYLIELATGKTKRLTTNVGSDNSPAWHPNSRTI
                     AFISKREGDYAQLHQIDIDGGEASLITEMPMGAAAPKYFPDGKRIAFVSTILPAYEND
                     FDGMKKNLKERKDSKMSAKVSENRFFRYWDTWLTDGYVPHLYSFHIETKERKDLTPGL
                     NELVSSDGSLDYDISPDGQWITFTRNSSNPPYAQLNSDVFLFKAGEYASAVNITADNL
                     DDDGGAVFSKDGKSLWYSRSFEKNHPDL"
     gene            1079..1756
                     /locus_tag="PK937_16540"
     CDS             1079..1756
                     /locus_tag="PK937_16540"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNH34234.1"
                     /translation="MSNPLLWVWEAVQKKAARVLAERLNRDLISYHLRTRNNMENLKR
                     ALKPGDVILVEGNKLISRVIMAVTHSTWSHCALYVGDGKIIDPLPSTGTVVNPVEALA
                     HLNIRVCRPFGLSGEAIDKVVSFAMSHVGRGYDHINISNLLFSFFKKKVDYTEFLGDI
                     SGSNEVCSGLIAEAYNQVGFPVLSGVNFSQIVPGDYDLSPNFEIIKFNKIYFNKNESL
                     VAREWHG"
BASE COUNT          510 a          371 c          435 g          545 t
ORIGIN      
        1 tcagatcggg atgatttttt tcaaacgaac ggctatacca caacgattta ccgtccttgg
       61 aaaaaaccgc accgccgtcg tcatcgagat tatctgccgt gatattgacc gccgaagcgt
      121 attcgccagc cttaaaaaga aaaacatcgc tgttgagctg tgcgtacgga ggattagaac
      181 tattacgggt aaaagtaatc cattgaccgt ccgggctgat atcgtaatcc aaagagccat
      241 cggaactgac caattcattg agtcccggtg taaggtcttt gcgttcttta gtttcgatgt
      301 gaaaactgta taaatgcggg acataaccgt ctgtcagcca tgtatcccaa taacggaaaa
      361 aacgattttc agaaacttta gccgacattt tggaatcttt gcgttctttt aagttttttt
      421 tcatcccgtc aaaatcattt tcataagcgg gcaatatcgt actcacgaaa gcaatgcgtt
      481 ttccgtcagg aaaatattta ggagcggcgg cgcccatcgg catctctgtg atcaaggagg
      541 cttcgccacc gtcaatgtct atctgatgaa gttgggcgta atcgccttca cgtttggaaa
      601 taaaagcgat cgttcggctg ttggggtgcc atgcaggcga gttgtcgctg ccaacattgg
      661 ttgtaaggcg ttttgttttt cctgtggcca attcgatcaa atacagatct gaattacctt
      721 tgttttcttt cacattgtag tctgtgacaa caaaacaacc atatttatta tcgggcgaca
      781 cggtgaaacc cgacagacgc ttaaatgccc ataagtcgtc agcagtaatt gggcgtttac
      841 cttgcgcctt tagcgtgtat ccaaataaca aaaaaatagt gatcatcaaa taataacgac
      901 gtacttgagg catggtaagc tcctttgatt atcgtgggct agggataaaa tgacccgaca
      961 acatatccat tccacaactt ttatgcaact acatagatat tgaaaatgga aggcaaaaga
     1021 agtacaataa tagcaatagc ggattttgcg atgtattact tatgtttgga ttttgaaaat
     1081 gtcaaaccct ctgctatggg tgtgggaagc cgtccagaaa aaagccgcac gggtacttgc
     1141 cgaaagactc aatcgggatc ttatcagtta tcacttacgt acacgcaata acatggaaaa
     1201 ccttaagcga gcgctgaagc ccggtgatgt tattttggtt gaaggtaata aattgataag
     1261 ccgcgttatt atggccgtaa cccattcgac atggagtcat tgtgcgcttt atgtgggtga
     1321 cgggaagatc attgatccgc tgccttcgac ggggacggtc gtcaatccgg ttgaggcgct
     1381 tgcgcacctc aatattcgag tttgccgtcc tttcggactg agtggtgagg ccatagacaa
     1441 agtagtttct tttgccatgt cacacgtcgg gcggggttac gatcacatca atatcagtaa
     1501 cctgcttttc agttttttta aaaagaaagt ggattatact gagtttttag gagatatttc
     1561 cggttccaat gaagtatgct ccggcctgat cgcggaagcg tacaatcagg tgggctttcc
     1621 tgttttgtcg ggagttaatt tttcacaaat cgtacccggt gattacgatt tgagtcccaa
     1681 ttttgagatt attaagttta ataaaattta ttttaataaa aatgaatctc ttgtagcgcg
     1741 cgaatggcac ggctgaaggg ctggggattt ttaagaggag ttttgggtat gaaatggttg
     1801 aaatacggca tgattttttt ggccgcatca gtttcacttt ttgcgaaaga tgatttggac
     1861 g
//
LOCUS       DAOQGF010000259         1853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_44312_length_1853_cov_8.348721, whole genome shotgun sequence.
ACCESSION   DAOQGF010000259 DAOQGF010000000
VERSION     DAOQGF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44312_length_1853_cov_8.348721"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1322)
                     /locus_tag="PK937_16545"
     CDS             complement(279..1322)
                     /locus_tag="PK937_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNH34235.1"
                     /translation="MKKNQVLFFCFFVALLDGCAPKSKNIWVIQEAVTKERLRGISAV
                     SERVVWASGNHGTCIRTTDGGSSWEKINIPNSDSLDFRDIEAFDADTAYVLSIGYGRQ
                     SRVYKTTDGGKSWQLQFLNTDTTAFFDAFAFWNSQDGIAVSDAVDGRWILVSTHDGGT
                     SWNRFPSQQSPLALSGEGAFAASGTCITTYGDSMVWIGSGVNAARVLSSNDRGKNWRY
                     SETPVAHSNGTSGIFSIAFYDEKKGVVCGGDFKDEKNNTNSLAFTADQGITWQIVNNK
                     LPQGFRSSILWLTDKILIAVGPNGSDISYDSGMTWNFLGDTGFHTADAGLRNKTIWAA
                     GDSGRVGKLDLDM"
BASE COUNT          554 a          383 c          325 g          591 t
ORIGIN      
        1 ttgactacgt tattaataat tttgcaatcc tgatatatca aaaatctctt ttccatttct
       61 cttagcggct ttgcgagaaa atatatgtac tagctaaagc gcaaaggtgc aaaaaattat
      121 ccatgacctc atggttgaat tgatatgtgt tttaaagaca agatagtgat tcattgacta
      181 cgttattgat aattatgcga ctctaaataa ttgaattaac aaattagtcc aaaagctttt
      241 ctgtctgtta gtgtactgtc cattgcgatt tttaaatcct acatatccaa atctagtttt
      301 cctacgcgtc cgctgtctcc tgcggcccat atcgttttgt tgcgcaaacc ggcatccgcc
      361 gtatgaaaac ccgtatcgcc taaaaaattc caagtcattc ctgaatcata agaaatatcc
      421 gaaccattgg gccctacggc gatcaatatt ttatctgtca accaaagtat ggaagatcga
      481 aaaccctgag gtagtttgtt gtttacgatt tgccaggtaa taccctgatc ggcggtgaaa
      541 gcaagtgagt ttgtattgtt tttttcatct ttgaagtctc cgccgcatac cacacctttt
      601 ttttcatcat aaaaagcgat ggaaaaaatc cctgaagtac cattggagtg tgcgactggc
      661 gtttctgaat atcgccagtt tttcccacga tcatttgacg aaagtacgcg tgccgcattg
      721 actccgctcc ctatccaaac catcgaatcc ccgtatgtcg taatgcatgt cccgcttgct
      781 gcaaacgcac cctctcctga aagtgctaac ggcgattgtt gagatggaaa gcgattccag
      841 ctagttcccc catcgtgtgt agatacgagt atccagcgtc catccactgc atcactgacg
      901 gcgatgccat cctgtgagtt ccaaaacgca aaagcatcaa aaaaagccgt tgtatccgtg
      961 tttagaaatt gcagttgcca cgattttcca ccatcggttg tcttgtaaac acgtgattga
     1021 cgtccataac ctatggataa aacatacgcc gtatcggcat caaacgcttc aatgtcacga
     1081 aaatcaagcg aatcagaatt cggaatattt attttttccc aagacgatcc tccgtcggtt
     1141 gtacgaatac acgtcccgtg attgccgctg gcccaaacaa cgcgctcgga gacagcgctt
     1201 attcctcgta aacgttcttt agttacagcc tcttgtataa cccaaatatt ttttgatttg
     1261 ggtgcgcatc cgtctaataa ggctacaaaa aagcaaaaaa ataatacttg gttttttttc
     1321 atacgttttc taacagattt agtaagtcct aagtaagatt aatttagcgc tgaaaggata
     1381 tctccaatct atattttttg agattgcaat acaatgagct tcggctccct tttacaatga
     1441 tttagtgatt tttaaataat attttttgtt atttaggata gttgactgat taaaaaaatt
     1501 aaagtaaaat acttagttat aaaatattag aaaaattcgt atcatttgag tacaattatg
     1561 aggtatttta tatcaaaatt ccaactatga aacagtcttg tgttgtattt attaggttta
     1621 cgcttggcac ataaaatgca aatttacaaa agcttttaat gaatttactt aaggtctatt
     1681 ttttataaaa acttttattt tcaaacaaat aagtttagag acatgaaaaa atttttactc
     1741 cccattgtgt ttttgctcgg ctgcatcagc gcatcagcgt atgcatcggc ggaaaataaa
     1801 accttagcct cttggtcgaa gtcaaatctc aattcgatca aacccatggc tgt
//
LOCUS       DAOQGF010000260         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_50297_length_1724_cov_4.346315, whole genome shotgun sequence.
ACCESSION   DAOQGF010000260 DAOQGF010000000
VERSION     DAOQGF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50297_length_1724_cov_4.346315"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK937_16550"
     CDS             complement(<1..302)
                     /locus_tag="PK937_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34236.1"
                     /translation="MSEIILKINRPTLRVVLNGTVPITGGGGGASFPSNQAANITKTD
                     RLYAKIGADEKYETIDQITKYNLGDKTWLNSWLDQTPFGGFELSRMFYTTRWAEADA"
     gene            complement(299..673)
                     /locus_tag="PK937_16555"
     CDS             complement(299..673)
                     /locus_tag="PK937_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34237.1"
                     /translation="MAKRYETDPNLDFEIPRDSIFNLPMTWEYDDGTAFDLTGCTIYF
                     VAMSDPEGAIASAIINHFWTTHTDATAGETTMTLTADTTASGTDKTLGTYYYNMKVVK
                     SGVNLATKLGRVTIAPNPCKDA"
     gene            complement(673..1164)
                     /locus_tag="PK937_16560"
     CDS             complement(673..1164)
                     /locus_tag="PK937_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34238.1"
                     /translation="MYRNALYEIGNLLQATDGISRVYIQQRDFNDQFDITNTIGGLQK
                     PTDVLAVVKTTGVNDLYKPQDFIALGFKMGFTIELFAGVDKVSDYSKSSESSFDRACF
                     NVLKKFMDVYHINIIHPDDSEGTLTIESISGVTPRDMTVIEELNKSAHHITFTLEAWE
                     TLT"
     gene            complement(1164..>1724)
                     /locus_tag="PK937_16565"
     CDS             complement(1164..>1724)
                     /locus_tag="PK937_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34239.1"
                     /translation="IDSTGNIRIFGGTTPYLAKVARRAAMVKPPISNKLTSRSGRLVA
                     SLNNKTKAGQIEAIRKIEMNGSGVTLTYGTSTPYALIHDKGGTINVSAHQRTSKFSAT
                     PIRSDLSTRDTIFKYRFAGNNKRVTSSKNFNKKNNIVSLTYKVKGYSINMPARPYVSK
                     ALLELPKKMQKLFDMAYDKTLGRILK"
BASE COUNT          449 a          390 c          333 g          552 t
ORIGIN      
        1 gcgtcggctt ccgcccatcg tgtcgtataa aacattcgcg ataattcaaa gccgccaaaa
       61 ggcgtttgat cgagccaaga attaagccaa gttttatctc ccaaattata tttagtgatt
      121 tgatctatcg tttcatattt ttcatctgcg cctatttttg cgtaaagcct atcagttttt
      181 gttatgtttg cggcctgatt agatgggaaa gaggcaccgc cgccgccacc ggttataggg
      241 accgtaccgt tcaacacaac acgcaatgtc ggtctgttta tttttaaaat tatctcgctc
      301 atgcgtcctt acaaggattc ggcgcaatcg ttacgcgccc aagtttagtc gctaagttca
      361 ctccgctttt aacgactttc atattgtagt aatatgtgcc gagcgtttta tctgttccag
      421 acgctgttgt atcagccgtt agcgtcattg ttgtttcgcc cgccgtagca tcggtatggg
      481 tagtccaaaa atgatttata atcgccgatg caatagcccc ttcggggtcg ctcattgcaa
      541 caaagtaaat cgtacatccc gttaaatcga aagccgtccc gtcgtcatac tcccaagtca
      601 ttggcaaatt aaatatcgaa tcacgcggaa tttcaaaatc taagttcggg tctgtctcat
      661 atcttttagc cattacgtca acgtctccca cgcctcaagc gtaaatgtta tatgatgtgc
      721 gcttttgttt agctcctcta ttaccgtcat atcacgcggc gtaacacccg atatgctttc
      781 tattgtcagc gttccttcgg aatcatccgg atgtattatg tttatatgat aaacgtccat
      841 aaatttttta agcacgttga aacacgcgcg atcaaacgac gactcagatg acttactata
      901 gtcgctgact ttgtcaacgc cggcgaacaa ttcaatcgtg aatcccattt taaaaccaag
      961 cgcgataaaa tcttgcggtt tatacaaatc gtttacgccg gttgttttaa cgacggccaa
     1021 cacgtcggtt ggcttttgca acccgccgat agtatttgta atgtcaaatt gatcgttgaa
     1081 atcgcgctgt tggatgtaaa cacggcttat accgtccgtc gcttgcaaca aattgccgat
     1141 ctcatataat gcgttgcgat acattactta agtattctcc ccaaagtttt atcatacgcc
     1201 atgtcgaaaa gcttttgcat tttcttaggc aattctaaca acgccttaga tacgtaagga
     1261 cgcgccggca tatttatgga gtatccctta actttatatg tcaaagaaac aatgttgttt
     1321 ttcttgttaa aatttttaga gctcgtaact ctcttattgt tgcctgcaaa cctatattta
     1381 aaaatagtat cgcgtgtaga taaatcggat cgtatcggtg ttgcgctaaa ttttgatgtt
     1441 ctttgatgcg ctgatacatt tatagtgccg cccttatcat gtatcaaagc gtaaggcgtt
     1501 gatgtaccat aagtcaaagt gacgccggag ccattcattt caatttttct aatagcttca
     1561 atttgcccag ctttagtttt attatttaaa gaagcaacaa gtcgtccgct tcttgatgtt
     1621 aacttattag atattggcgg ttttaccatc gcagcacggc gtgccacctt agctaaataa
     1681 ggagtagtgc cgccgaaaat tcttatatta cccgtgctat caat
//
LOCUS       DAOQGF010000261         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_51232_length_1705_cov_4.107273, whole genome shotgun sequence.
ACCESSION   DAOQGF010000261 DAOQGF010000000
VERSION     DAOQGF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51232_length_1705_cov_4.107273"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK937_16570"
     CDS             complement(<1..454)
                     /locus_tag="PK937_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34240.1"
                     /translation="MRIILTLVFAISLASCSISNKTISLAPTSLKLSANDTINLPSYA
                     SKYSEYDGVFLEIDESMEHNASDLMMGLNEWSFFYIRKVRYVIFNPDNQRLSTFSVHV
                     ESNQVLKNAFMRTISPDGKIKRFNMLHLKSEKNSDHSQTYRFVYPDVVK"
     gene            complement(451..>1705)
                     /locus_tag="PK937_16575"
     CDS             complement(451..>1705)
                     /locus_tag="PK937_16575"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNH34241.1"
                     /translation="INYLTPLAHNPFERIRAVVLTKLQNAKREPINPYTPASFTSWYN
                     GKLAQNGLTDDRHDSLAQAITRGLEEPMDKLRAIHNHVKNRIRYIAVADSFHAIIPHR
                     SKKVLELNYGDCKDRALLLMLLARSAGIPVRMALVSTTPRLRFDGVHTSFFNHVINVY
                     EADGKRFYFDPTAKYCEFGNLPPSDFNGRVLILNEADPVYTDEPPLPRKLSIEVEVNG
                     EMDSLRAAKASIKLRNEYLSSARHARYELSGQTLENYLSRMINSNFSKISMDQFHFEN
                     ETDSTMTFNAKADLSEFIIQTATRKYIPQAPFQSVSSQTGERIKDAWPVYMKNPEMLG
                     MMLRLKIGDAKVNYKDIALGDKGVVWFSNQTSLDASRGMLETRYEFVQQPLWYDGVER
                     QSYLEFCKQYLKLKTSLITITGNTP"
BASE COUNT          456 a          357 c          395 g          497 t
ORIGIN      
        1 ctttgaccac gtcgggataa acaaatcggt aggtctgtga atggtccgaa tttttttctg
       61 atttgaggtg cagcatgtta aaacgcttga tttttccgtc gggagaaatc gtacgcatga
      121 atgcgttttt caatacttga ttggattcga cgtgcaccga aaaagtgctc aacctttgat
      181 tgtcgggatt gaaaataacg taacggactt ttcgaatata aaaaaatgac cattcgttga
      241 gccccatcat caagtccgat gcgttgtgct ccatggattc gtctatttcg agaaacacac
      301 catcatactc cgaatatttt gaagcatacg acggaagatt gatcgtatcg ttagcgctca
      361 gttttaatga tgtcggtgcg agggatatgg tcttattgga gatcgagcag gatgccaggg
      421 atatagcaaa gaccagcgtt agtattattc tcatggcgtg tttccggtta tagtgataag
      481 cgatgtttta agtttcagat attgtttaca aaattcgaga taagattgtc gctccacccc
      541 atcataccaa aggggttgct ggacaaactc atagcgtgtt tctaacattc cccgcgacgc
      601 atccaatgaa gtttgattgc taaaccaaac aacgcctttg tctccgagcg cgatatcttt
      661 ataattcacc tttgcatcgc ctattttcaa tcgcagcatc ataccaagca tttccgggtt
      721 tttcatgtac accggccacg cgtcttttat tcgttcaccc gtttgggacg aaacggattg
      781 aaacggagct tgcgggatat atttgcgtgt tgcagtttga ataataaact ccgacaaatc
      841 cgctttagcg ttaaatgtca tggttgagtc tgtctcgttc tcaaaatgga attgatccat
      901 gcttatttta ctgaaattgg agttgatcat tcgggataaa taattttcaa gcgtctgtcc
      961 ggaaagttca taccgagcat gacgggccga cgataaatat tcattacgaa gcttgatgct
     1021 ggctttcgcg gcgcgcaaac tgtccatttc tccgtttact tccacttcga tggataattt
     1081 tcgcggcaag ggcggctcgt cggtgtaaac cgggtctgcc tcgttgagaa tcaatactcg
     1141 tccgttgaaa tcagacggag gaagattgcc aaattcacaa tatttagccg taggatcaaa
     1201 ataaaaacgt ttcccgtcgg cttcatagac attgatcaca tgattaaaaa acgatgtatg
     1261 gacgccatca aaacgaagtc tgggcgtggt cgaaacgagc gccatacgaa caggtatacc
     1321 tgcagaacgc gccaaaagca tcagcaacaa tgcccggtct ttacaatcac cgtagtttaa
     1381 ttcgagtact tttttggagc gatgcggtat gatcgcatga aaggaatccg ctacagcgat
     1441 gtatcgaata cgatttttga catgattatg tattgctctg agtttatcca taggctcttc
     1501 taatccccgc gtgatagcct gcgccagaga gtcatgacga tcatcggtca aaccgttttg
     1561 agcgagcttt ccgttgtacc acgatgtaaa tgaagccggt gtgtagggat tgatcggttc
     1621 gcgttttgcg ttttgcagtt tggtcaaaac tacggcacga atgcgttcaa acgggttatg
     1681 tgccaagggt gtaagatagt tgata
//
LOCUS       DAOQGF010000262         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_53507_length_1664_cov_7.376631, whole genome shotgun sequence.
ACCESSION   DAOQGF010000262 DAOQGF010000000
VERSION     DAOQGF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53507_length_1664_cov_7.376631"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK937_16580"
     CDS             <1..419
                     /locus_tag="PK937_16580"
                     /inference="COORDINATES: protein motif:HMM:NF026866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 23 domain-containing
                     protein"
                     /protein_id="HNH34242.1"
                     /translation="NWSNDLPFSDKGRTASAEIGFGQNLIGFNVYTNEDLNRKAAYES
                     VGEGTDYDSPIGGRNPEGTYVNGKPYFSAFYYARRSGGNVTRAGLNQPWVQDLFQNVV
                     AHKGLLRLLGGKAPYFRPGNYGSVYGYHGRYNPFIY"
     gene            382..1038
                     /locus_tag="PK937_16585"
     CDS             382..1038
                     /locus_tag="PK937_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34243.1"
                     /translation="MGIMDDTIHSSIKKKKILMKKQQFILSFICVLSLGACVITRYHD
                     DFWDPTLILKLQDFKVNRNGYFKLINPPPINKYDGFRDFILYENGFFAGHGFRVSQDR
                     MQKAEFKTPQVDWGIYNIGKDSVIIIQHFYPAVIIPGFFSAIDEYAVSSVKGYVSDAG
                     TILIYSRTEHVLRPKKTDEKISHLTFQPPLEFKFIGHDSLPSSDGNWLEEWLKKKKEY
                     "
     gene            1279..>1664
                     /locus_tag="PK937_16590"
     CDS             1279..>1664
                     /locus_tag="PK937_16590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNH34244.1"
                     /translation="MYDGLNSIAVKRKGGGTTEYVYGPTGLIASKVNGTWYFVLKDHL
                     GSARTIVDQSGTTKASIDYAPYGAILRTSGSPNIAYQFTGQEYDSTSGLWNYKARMYD
                     AELGVFYAVDPAGQFAGAYIGLGNNPV"
BASE COUNT          503 a          284 c          384 g          493 t
ORIGIN      
        1 tcaactggtc gaacgatctg cctttttccg ataaaggaag aactgcatca gcagagattg
       61 gatttggaca aaacttaata ggatttaatg tttacacgaa tgaggatttg aatagaaaag
      121 cagcatatga aagtgttgga gaaggcactg actatgattc gcctatcggc ggaagaaatc
      181 cggaaggaac ttatgtcaat ggaaaaccgt atttcagcgc tttttattat gcaagacgta
      241 gtggcggtaa cgtgacgcgt gcaggcctga atcagccatg ggtacaagat ttattccaaa
      301 atgtggtcgc tcacaaaggg ttattaaggc ttttaggagg aaaagctcct tattttcgac
      361 caggtaatta cggatcagtt tatgggtatc atggacgata caatccattc atctattaaa
      421 aaaaagaaga tattaatgaa aaaacaacaa ttcattttat cgtttatttg cgtgctatca
      481 ttaggagcat gcgtgattac acgatatcac gacgattttt gggatccgac gctcatacta
      541 aaactgcaag atttcaaagt gaatagaaat ggttatttta agttaataaa tcctcctcca
      601 attaataaat atgatggatt tcgtgatttt attttatatg aaaatggttt ttttgcaggt
      661 catgggtttc gtgtttcaca agatcgtatg caaaaagcag agtttaaaac tcctcaggtg
      721 gattggggaa tttataatat tggcaaggac agtgttatta ttattcagca tttttacccg
      781 gcggtcataa ttcctggttt ttttagcgcg atcgatgaat atgcggtgtc atctgtaaaa
      841 gggtatgttt cggatgctgg tactattcta atatactctc gcacagaaca tgtattaaga
      901 ccaaaaaaaa ccgatgaaaa aatcagtcat cttacatttc agccaccgct cgaattcaaa
      961 ttcattgggc acgattcgct accatcatcg gatggaaatt ggcttgagga atggttaaaa
     1021 aagaaaaagg aatattgata gaataatggt ttgttgttat ttacaattgc cgatgaaatc
     1081 ataactcgat atttctttac atcaattgat acgccaattt tttatatttc attgtacata
     1141 ttctggagca agttccgtgg aagagagttc gttctgaata caaaacactt aagcgttatg
     1201 acctaagttt gcaggatttg aaagaccgag attggaaagt tgtttatcct taagatttga
     1261 agtcgggtct tcatatattt gtacgatggc cttaatagca tcgccgtaaa acgcaaaggg
     1321 ggtggtacta cggaatacgt ctatggcccg acgggactga tcgcatcgaa ggtcaatggg
     1381 acgtggtatt ttgtactcaa agatcatctg ggttcggcgc gtacgatcgt ggatcaatcg
     1441 ggcacgacga aagcttcgat agactatgcg ccttatggag cgatccttcg cacatcgggc
     1501 tcgcccaata tcgcgtatca gtttaccgga caggaatacg acagcacgtc cggattgtgg
     1561 aattataagg cacggatgta tgacgcggag ctgggtgtgt tttatgccgt ggatccggcg
     1621 gggcagtttg caggtgcata catcggatta ggaaataatc ctgt
//
LOCUS       DAOQGF010000263         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_54653_length_1645_cov_7.444654, whole genome shotgun sequence.
ACCESSION   DAOQGF010000263 DAOQGF010000000
VERSION     DAOQGF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54653_length_1645_cov_7.444654"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK937_16595"
     CDS             <1..354
                     /locus_tag="PK937_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34245.1"
                     /translation="HRDEEVNAAMLEWKNLVTGETYKQILRQVLFLVRKENLSRCIWN
                     LNSVGNINKEDLKWSFQFWLPAMAQCGVKSFAVIVNEQHFLHLAIASYLEKQDIVEYG
                     MFEKSASAMYWLQSH"
     gene            complement(357..968)
                     /locus_tag="PK937_16600"
     CDS             complement(357..968)
                     /locus_tag="PK937_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34246.1"
                     /translation="MEIRELTVDDIAQAARISVESFDDYNIVMSKMGENYLRTVFFPA
                     IVQRQDTGAMGAFQDGKMLGYYCYMLDFHRFHADLQNKNFFRNVRFTLSAMMRGRLSI
                     KDLYNVFRAGAWIRRETAHIPVQVGPVAVARDIKGTPTGGMVTFTVVRAVLKKLQQQG
                     IKACWSTVDLRNPSQHVSTAVGFHEKSRIKLWGIEEALHVKKF"
     gene            complement(1041..1442)
                     /gene="mscL"
                     /locus_tag="PK937_16605"
     CDS             complement(1041..1442)
                     /gene="mscL"
                     /locus_tag="PK937_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNH34247.1"
                     /translation="MGMMQEFKQFASRGNVVDLAVGIIIGGAFGKIVSSAVSDLLMPP
                     LGWMIGGLSFTDLKFTFKTELLDVKPVTINYGNFLQTCVDFLIVAFAVFLLIKAMNRF
                     HQKQETAPAAPPAPSKQELLLEEIRDLLKKK"
BASE COUNT          457 a          372 c          352 g          464 t
ORIGIN      
        1 caccgagatg aagaggttaa tgccgccatg ttagaatgga aaaatcttgt aaccggtgaa
       61 acgtataaac agattttacg tcaagtattg tttcttgtgc gtaaggaaaa cttgtcccgt
      121 tgcatttgga atctgaacag tgttggaaat attaataaag aagacctgaa atggagcttt
      181 cagttctggc ttccggcgat ggcccaatgc ggtgtgaaat cgtttgccgt catcgtcaat
      241 gaacagcatt ttttacattt ggccattgct tcatatttag aaaaacaaga catcgtagaa
      301 tatggtatgt ttgaaaaaag cgccagtgcg atgtattggc tacaatcgca ttgaaattaa
      361 aatttcttta cgtgcaacgc ttcttcgata ccccataact tgatacgtga tttttcgtga
      421 aatccgacgg cggtagatac atgctgggaa ggattgcgca aatccacggt actccaacac
      481 gctttgatcc cttgttgttg caactttttt aatacggcgc gcacgaccgt aaaggtcacc
      541 atacctccgg tcggagtgcc tttgatgtca cgcgctacgg caaccggccc gacttgcacc
      601 ggtatgtggg ctgtttcacg ccgaatccat gctccggcac gaaatacatt ataaagatct
      661 tttatgctca accgtccgcg catcatagcc gaaagcgtaa aacggacgtt tcgaaaaaag
      721 ttcttatttt gcagatcggc atgaaaacga tggaaatcga gcatataaca ataataaccg
      781 agcattttcc cgtcttggaa tgcccccatc gcacccgtat cttggcgttg aactatagcg
      841 ggaaaaaaaa cggtccgaag ataattttct cccattttac tcataacgat attgtagtcg
      901 tcaaacgatt cgacagagat acgcgcggct tgtgcgatat cgtcaacggt tagttctcgg
      961 atttccatag ttttgcgaat gcggttttaa aatatgaggg agcggcacga atccgccccc
     1021 atacaatgag tccaagtttt ttatttcttt ttgagtaagt ctctgatttc ttcgagcagc
     1081 aattcttgtt tgcttggcgc cggaggtgca gccggcgctg tttcctgttt ctgatgaaag
     1141 cggttcatcg ctttgatcag aagaaacacg gcaaaagcca cgatcaaaaa atcaacacac
     1201 gtttgcaaaa agtttccgta attgatggta accggtttga catcaagtaa ctccgttttg
     1261 aacgtaaatt tcaaatccgt aaaactgagc ccgccgatca tccatccgag cggaggcatt
     1321 agcagatcgc tcacggccga tgaaacgatt tttccgaaag cgccgccgat gatgatacct
     1381 acggcaagat cgaccacatt gccacgcgaa gcaaattgct taaattcctg catcataccc
     1441 atacaatatt ctcctcgtta tgttggcaga gcggttaaaa tttgtacagt agcccggcgc
     1501 caaaaacttc tttgatctgc catttgttgg aaacatcctt atcgtaaaca aattgtgccg
     1561 tcaaattggc gctgacatat tcattcacct tcatcagaat caggttttcc cagttgacat
     1621 caacttcttt gatttttttg taact
//
LOCUS       DAOQGF010000264         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_56634_length_1614_cov_2.291212, whole genome shotgun sequence.
ACCESSION   DAOQGF010000264 DAOQGF010000000
VERSION     DAOQGF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56634_length_1614_cov_2.291212"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PK937_16610"
     CDS             complement(<1..391)
                     /locus_tag="PK937_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNH34248.1"
                     /translation="MKLSVVIPVYNEKNTILQVIKTVRNVDIGNTVMEVVVVDDCSTD
                     GTTELLRSQTSTDILKFYFHQHNMGKGAAVRTGFNQTTGDFIIVQDADLEYDPNEYGK
                     LLEPLLSGKADVVFGTRFAGNDAHRVLY"
     gene            complement(388..>1614)
                     /locus_tag="PK937_16615"
     CDS             complement(388..>1614)
                     /locus_tag="PK937_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNH34249.1"
                     /translation="RKREQKGSRNTFQSFVTPPTYNGKETMNIGICALGDVGSDTGGQ
                     TYIRNFVRMASELSDGDCIFFFVGSTDVPWMQSDHSNVKIITVPFSSPGLYRRLAGEH
                     IILPELIRQYDIDVMYYPNNFTSLNCPVPCVVAIRSTLYYHYPYAIDGLRLRYRKAMT
                     PRSARQAVKIICPSNDIKNDILNYIGVDSQKIEVVHHGIDVKLFGRSFTDKEFLSVAA
                     PLGIRKPFLMYVSALWEYKNQDILIESFARLIQDHSIPHQLVIVGKGLNTANSYEQKL
                     RDLPKQLGIENRVIFTGFQSHDVLKYLYCNTDVFVFPSSYESFGNPLFEAMAAGVPIV
                     AANTHSFPEMAGDAALLINPRDHRAMADAIYSVITDTKLKAQLIANGKKRIIAFDWNV
                     CFQKTMNIIRKAGKEK"
BASE COUNT          465 a          340 c          343 g          466 t
ORIGIN      
        1 aatacaagac ccgatgggca tcgtttccgg cgaatcgcgt accaaaaaca acatctgctt
       61 ttccggacaa cagaggttca agtaattttc cgtactcatt tgggtcatat tccaaatcgg
      121 catcctgaac tatgatgaaa tcacccgtag tttgattaaa tccggttcga accgcagcgc
      181 ctttacccat gttatgctga tgaaaataaa atttaagaat atcagttgat gtctgcgagc
      241 gtagcaattc tgtagtgccg tcggtagagc agtcatccac taccacgact tccatgacag
      301 tgttgcctat gtcaacattg cgaacagttt tgataacttg aagtatagta ttcttttcgt
      361 tataaaccgg aataaccacg gacaatttca tttttctttg ccggcctttc ggataatatt
      421 cattgttttt tgaaaacaga cattccaatc aaacgctata atacgttttt ttccgttagc
      481 aatcaattgc gctttgagct tagtatctgt tataaccgaa tagatagcat cggccattgc
      541 gcgatgatct cttggattta ttaatagggc tgcgtcaccg gccatttcgg ggaaagagtg
      601 agtatttgcc gcgacgatag gtacacctgc ggccatcgct tcaaaaagtg ggttaccgaa
      661 gctctcatac gaagacggaa aaacaaaaac atctgtattg cagtataggt acttcaatac
      721 atcgtgcgac tgaaatcccg taaaaatcac acggttttca atgcccagtt gtttcggcaa
      781 atcgcgtaat ttttgttcgt aagaattggc tgtatttaat ccttttccaa caattactaa
      841 ttgatgaggt atagaatgat cttggatcag ccgggcaaaa ctttcgatca gaatatcttg
      901 gtttttatat tcccataaag cagaaacgta catgaggaaa ggtttgcgaa tccccagtgg
      961 tgcagcgacg gataaaaatt ccttatccgt gaacgagcgt ccaaataatt tcacgtctat
     1021 accgtgatgt acgacttcaa ttttctgcga gtccacgccg atatagttga ggatgtcatt
     1081 ttttatatca ttagacgggc aaatgatttt gacagcttgg cgcgcactgc gcggtgtcat
     1141 cgcttttcga taccgaagcc gtaacccgtc aatcgcatag ggataatggt agtataatgt
     1201 gctgcgaatg gcgactacgc acggtacagg gcagttaagc gacgtaaaat tatttggata
     1261 gtacattacg tcaatgtcat attggcgtat caattccggc aggatgatgt gttcaccggc
     1321 gagtcggcga tataaacccg gagaagagaa aggaacggta ataattttta cattagaatg
     1381 atccgattgc atccaaggca catcggtcga accaacgaaa aaaaaaatac aatctccgtc
     1441 gctcaattca ctggccatgc gaacaaaatt gcgaatatac gtttggccgc cagtatcgga
     1501 gccaacatca cctaaagcac agattccgat attcatggtt tcttttccgt tataggtagg
     1561 tggtgtaaca aatgactgga aagtatttcg gctacctttt tgttcccttt ttcg
//
LOCUS       DAOQGF010000265         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_60166_length_1559_cov_2.251995, whole genome shotgun sequence.
ACCESSION   DAOQGF010000265 DAOQGF010000000
VERSION     DAOQGF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60166_length_1559_cov_2.251995"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1559)
                     /locus_tag="PK937_16620"
     CDS             complement(<1..>1559)
                     /locus_tag="PK937_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34250.1"
                     /translation="REAFVATPAGDFFKNTIPVAMIDFGRHAYWVITANDGIYTYNDS
                     TGLIKKLKTNMPLENLKISNAVRMKNGEIIVTSTLQGIFVMDSTGRIRMHADNRDARG
                     LSDTKFAFHATDGSVWFGHNNGLSVSDFYSPITFMRNEKGIDGAVTGVMSFGGKIVAG
                     TTQGLWESVSDEILPELLTFRKIKGTEFSVYGMDTMYTRLIIYGGDFAILDTNRKLLF
                     QTLEIGPRAIAKLPSRHNTILAAHRNILYAVDVFSFSMKPIHTFDDEVTAIVLDRSDQ
                     DGACYYWMSLRNRGVAYVRVDKNAILHIVAAHTMKGLNAYSNLSVLDNQTYILNGEEV
                     YRLTIDTESKVIRSNLDAMFTSVRGGVDINHILLRNKVFWIAKGYRFYKLIPLAANRW
                     QLDSTISIPGDPIYIDPQDSVTWFAGTEGVARWDHRVSRDYALGFPVLIRRIVLNNDS
                     LLRSGSISSATIFPESFEISYAFNSLTLYFSASHYHDISALRFSWKMEGFTNEWTDWS
                     EESRAIYTNLN"
BASE COUNT          461 a          373 c          309 g          416 t
ORIGIN      
        1 tcattcaggt tggtgtagat cgcacggctt tcttcactcc agtccgtcca ttcgttggta
       61 aatccttcca ttttccatga aaagcgcaaa gctgaaatat cgtgataatg tgatgctgaa
      121 aagtataacg tgagactatt gaacgcatag gatatttcga atgattcggg aaatattgtc
      181 gcactggaaa tcgaaccgga ccggagcaac gaatcattgt tgagaacaat acgacggatt
      241 agaacgggaa accccaatgc atagtcgcgt gaaactctgt gatcccatcg cgccaccccc
      301 tccgtaccgg caaaccacgt aaccgaatct tgcggatcaa tataaatcgg atcgcccgga
      361 atagagattg tagagtcaag ctgccaacga ttggcggcca acggtatcag tttgtaaaag
      421 cgatatcctt tggcaatcca aaacacctta ttgcgtaata agatatgatt aatgtccaca
      481 ccaccgcgca cagaagtgaa catcgcatcc agattactgc gaatgacttt cgattctgtg
      541 tctattgtca aacgataaac ttcttccccg ttcaagatgt aggtttgatt atctaaaacc
      601 gataagttgg aataagcatt caatcctttc atcgtatgcg ccgccacaat atgcaggatc
      661 gcatttttat cgactctgac ataggccaca cctcgattgc gcaaactcat ccaataatag
      721 catgctccgt cttggtccga tcgatcaaga acgattgccg taacctcatc atcaaaagtg
      781 tgaataggct tcatggaaaa agaaaacacg tccaccgcat ataaaatatt acgatgagct
      841 gccaaaatag tattatgtct acttggtaat ttggctatag cgcggggacc gatctccaaa
      901 gtttgaaaaa gtagtttgcg attggtatct aatatcgcaa aatcaccgcc gtagatgatc
      961 aaccgtgtat acatcgtatc cattccatag acggaaaact cggttccttt gatttttcgg
     1021 aacgttagta attccggcag gatttcgtct gaaacactct cccataaacc ttgcgtcgtg
     1081 ccggcaacaa tttttccgcc aaaactcata acgccggtaa cagcgccatc aatccccttt
     1141 tcgttgcgca taaacgtaat cggactataa aaatcagata ccgataaacc attgttatga
     1201 ccaaaccaaa cgctgccatc ggttgcgtga aaagcaaact ttgtgtccga gagtccacgc
     1261 gcatcgcgat tatcggcgtg catgcgaatg cggcccgtac tatccatgac gaatattcct
     1321 tgcaacgtac ttgtaacgat aatttctccg tttttcatac gtacggcatt agatattttt
     1381 aaattctcca acggcatgtt cgttttcaat tttttgataa gacccgttga atcattgtat
     1441 gtataaatac cgtcattggc tgtaatcacc caatacgcat gccgcccgaa atcaatcatc
     1501 gctacgggaa tagtgttttt aaaaaaatca cctgcgggcg ttgcaacaaa cgcctcacg
//
LOCUS       DAOQGF010000266         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_75741_length_1373_cov_5.345220, whole genome shotgun sequence.
ACCESSION   DAOQGF010000266 DAOQGF010000000
VERSION     DAOQGF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75741_length_1373_cov_5.345220"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..581)
                     /locus_tag="PK937_16625"
     CDS             complement(60..581)
                     /locus_tag="PK937_16625"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNH34251.1"
                     /translation="MDYKKYIREVPDFPKPGINFKDISTLVGNADAFRHVIRTMTEDA
                     AELKVDLVAGIESRGFIFGAAIAEKMGIGFIPVRKPGKLPYQTISASYALEYGENTLH
                     MHTDAIQPEQRVLIVDDLLATGGTVGATCALVEKLGGKVAAISVVIELSFLGAREKLN
                     SYKVMSLVDYSGE"
     gene            complement(602..874)
                     /locus_tag="PK937_16630"
     CDS             complement(602..874)
                     /locus_tag="PK937_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNH34252.1"
                     /translation="MNRAVITVSGIVQGVGYRRFVEKLAAECKLNGWVKNLENGDVYC
                     VVEGHDTELNQFLQYLRKGPTFARVTNVNVEWVHATSEFTSFMIKY"
     gene            complement(871..>1373)
                     /locus_tag="PK937_16635"
     CDS             complement(871..>1373)
                     /locus_tag="PK937_16635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNH34253.1"
                     /translation="LYNRRYINKKLDDEFRRAVRYKRPLSVIMFDADHFKSVNDTYGH
                     AFGDVVLKDIAAIILETARLVDICGRFGGEEFIAILPDTDIENAFVVADRIRKKVEEH
                     EFKDATNSIRRTVSVGISSLPDDLMNDQFTLIEWADKGLYYAKEHGRNQTVRYSDLPE
                     YKQIKG"
BASE COUNT          380 a          355 c          261 g          377 t
ORIGIN      
        1 gaataacagc taaaccaata ttccgcagga tagcgatctt caggtacaac aagcgccatt
       61 tattccccgc tgtaatccac taaactcatg actttatacg aattcagttt ttctcgtgca
      121 cctagaaacg acaattcaat tacaacgctt atcgcggcca ctttgcctcc caatttctct
      181 accaatgcgc acgtcgctcc gaccgtaccg ccggttgcca aaagatcgtc cacgatcaac
      241 acgcgttgtt ctggttggat cgcatcggta tgcatgtgca acgtgttttc accatactca
      301 agcgcatacg aagcagatat cgtttgatag ggcaatttcc ccggcttacg aacgggaata
      361 aatccaatac ccattttttc agcgatggcc gcgccaaaaa taaatccgcg cgattcgatg
      421 ccggcaacaa gatcaacttt cagttcagcg gcgtcttctg tcatcgtacg aataacatgc
      481 ctaaaagcat ccgcattacc taccaatgtc gaaatgtctt taaaattaat tcccggcttt
      541 gggaaatccg gaacttcacg tatatatttt ttatagtcca taatacgatg cacacttaaa
      601 actaatattt aatcataaaa ctggtgaatt cggatgttgc gtgcacccat tcaacgttta
      661 cattcgttac acgcgcaaac gtcgggcctt tgcgaagata ttggagaaat tgattgagtt
      721 cggtatcatg accttcgaca acgcagtata catcgccatt ctcgaggttt tttacccaac
      781 cgttcagctt acattcagca gcgagctttt ccacaaatcg gcgatacccg acaccttgca
      841 ctattccgct gacagtgatg acggcccgat tcatcctttg atttgcttgt actccggcag
      901 atccgaatag cgtaccgtct ggttacgacc atgttcttta gcgtagtata aacctttatc
      961 ggcccattcg atcaacgtaa actggtcatt catcagatca tcaggaagcg acgaaatacc
     1021 gacgctcact gtcctacgaa tggaattggt cgcatcttta aattcatgct cttcgacttt
     1081 tttacgaata cgatccgcca ctacaaatgc attctcaata tccgtatccg gaagaatcgc
     1141 aataaattcc tcgccgccaa aacgtccgca aatatctacc aatcgcgccg tttcaagaat
     1201 tatcgcggca atgtccttga gcaccacatc gccaaaggca tggccatagg tatcgtttac
     1261 tgatttgaaa tgatccgcgt caaacatgat aacggataaa ggacgtttat accgtacggc
     1321 acgacgaaat tcgtcatcga gttttttgtt gatgtatcgg cggttgtata act
//
LOCUS       DAOQGF010000267         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_76163_length_1369_cov_3.484018, whole genome shotgun sequence.
ACCESSION   DAOQGF010000267 DAOQGF010000000
VERSION     DAOQGF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76163_length_1369_cov_3.484018"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK937_16640"
     CDS             complement(<1..648)
                     /locus_tag="PK937_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNH34254.1"
                     /translation="MTIEQALKILEITNSNPNREEIKTAYRRLSKKYHPDATGSKDAT
                     KFLMINAAYERLINWSKSPEASSRTDVQDTEFETESELEVRIQAVNRAFDLLFQSYEE
                     YHNKIFEEMDNGIVNHLNSYNSNGDLKKRFTSDFELLIKNGMSNIISWFDKNIMGITS
                     AYDSWINGFLKDAYAVLKKSELEKWYKSVFFRKYVVFCFLFSLISIPFGIYLDFNL"
     gene            complement(951..>1369)
                     /locus_tag="PK937_16645"
     CDS             complement(951..>1369)
                     /locus_tag="PK937_16645"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNH34255.1"
                     /translation="DTPPVILKNLHRILKVRAYGYWGEHIWWNVQTGRAYSDLIGKQR
                     AYEIIVNAIIPYVALYAQRRGDKQLHDTVMTCMKTWASYEHNHIIGWMRKRLPGMPDR
                     VNAEYAQGLIELHKKCRVRNCSDCRIFQRMVYSDFI"
BASE COUNT          417 a          276 c          249 g          427 t
ORIGIN      
        1 gaggttgaaa tcgagataaa taccgaatgg tatactgatc aagctaaata gaaaacagaa
       61 aacaacatat ttcctaaaaa agaccgattt ataccatttt tctagttccg atttttttaa
      121 tactgcataa gcgtccttca aaaacccgtt gatccaagaa tcatacgctg atgtaatgcc
      181 catgatattc ttatcaaacc agcttataat attagacatc ccgtttttaa tcaaaagctc
      241 aaaatcagat gtaaatcttt tttttagatc tccgtttgaa ttgtatgaat tcagatgatt
      301 tactatgcca ttgtccattt cctcgaaaat tttgttatga tactcctcat aactttgaaa
      361 cagcagatca aacgctctat tgacagcttg gatacgaact tccaattcag attcagtttc
      421 aaactcggta tcctgaacgt ctgttctgct tgaagcctca ggtgattttg accaatttat
      481 aaggcgctca taagctgcat tgatcatcag aaattttgtt gcatcctttg atcccgtagc
      541 gtcaggatgg tattttttag ataacctgcg atatgcagtt tttatctctt cgcggtttgg
      601 gttcgaatta gttatttcga gtatcttcag tgcttgttca attgtcatag agtattcctc
      661 aagttctgta gttcaatgag ttaacaaggg attggaaata tttattgcat agatagagca
      721 aatatcgttt acaattaact aaataaatta tgtaattgaa gaacacaaaa aaagtaaaat
      781 attcgcccgt atgtaaagaa aaatgtagta aactaccacc gccaaaggcg gtggtagttt
      841 aagccgaaat aaaaaataaa acagctttat atgaaattat acttctctcc aaagtgatca
      901 atattatacg atattaataa atataaattt atcgtcacat gaactgcacg ctaaataaaa
      961 tccgaataca ccatgcgttg gaatatgcgg caatcactgc aattgcgcac acggcatttt
     1021 ttatgcagtt cgatcagccc ttgcgcatat tcggcattga cccgatccgg catgcccggt
     1081 aagcgtttcc tcatccagcc gatgatatga ttgtgttcgt acgatgccca cgttttcata
     1141 catgtcatga cggtatcatg cagttgttta tcccctcggc gctgtgcgta cagcgcgacg
     1201 tacggaatga tcgcattaac gatgatctcg tatgcgcgtt gctttccgat cagatcggaa
     1261 tacgcgcggc ccgtttggac attccaccaa atgtgttccc cccaataacc gtaggcacgg
     1321 acttttagaa tacgatgtaa gtttttaaga attaccggcg gcgtgtcgc
//
LOCUS       DAOQGF010000268         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_0685
            NODE_81321_length_1321_cov_7.233807, whole genome shotgun sequence.
ACCESSION   DAOQGF010000268 DAOQGF010000000
VERSION     DAOQGF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810317
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:22:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,331
            CDSs (total)                      :: 3,287
            Genes (coding)                    :: 3,273
            CDSs (with protein)               :: 3,273
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81321_length_1321_cov_7.233807"
                     /isolate="AMR_MDS_0685"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PK937_16650"
     CDS             complement(<1..458)
                     /locus_tag="PK937_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNH34256.1"
                     /translation="MKRILFFLMLGTLLIGSSSVFAQKPAWVDNPGSYSAENFVSVGV
                     AKDSRPDKAKEKAEKKSKAALEKLLRNKYPKKDVKGAMTSLRFEAYYQDPATKYYYVL
                     ALIPIETLDKDYAKQKMMDRNRSAAMGAVAMLNAQTKKDPDVIIVRVDEEV"
     gene            complement(500..>1321)
                     /locus_tag="PK937_16655"
     CDS             complement(500..>1321)
                     /locus_tag="PK937_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNH34257.1"
                     /translation="KVVVDCVNGAGGVIVPRLLKELGCNVVVINEEPHGVFPRNPEPS
                     VDNLKALEETVRQHHAHIGFAVDPDVDRLALVNEKGQAIGEELTLMIATDLVLSRKKG
                     PVVVNASTTMGIDQIATMYGVQTHHTRIGEINVSTKMEEIDSVIGGEGNGGVILPEIH
                     LGRDACTGIALALQYVLQKNKPFSECVAELPYFIMKKDKIELHDIDADAVMTKILKDQ
                     KKGRVDTVDGVKIIFEDGWIHFRKSNTEPIMRIIAEARTQERLDNMIQSFTSYFH"
BASE COUNT          343 a          306 c          265 g          407 t
ORIGIN      
        1 acctcttcat ctacacgcac tatgattaca tccggatctt ttttcgtttg cgcgttgagc
       61 attgctacgg cacccatggc agcagaacga ttgcgatcca tcattttctg tttggcataa
      121 tctttatcca acgtttctat aggaataaga gctaaaacat aataatattt tgttgccgga
      181 tcttgataat aggcttcaaa acgtaacgac gtcatagcac ctttgacgtc ttttttggga
      241 tatttgttac gaagcagttt ttcgagagct gctttagatt ttttttcggc tttttctttg
      301 gctttatcag gccggctgtc tttagcgaca cctacggaaa caaaattttc ggccgaatag
      361 gaaccggggt tatccaccca agccggtttt tgtgcaaata cggacgaaga cccaatcagc
      421 aacgtcccga gcatcaaaaa aaacaaaatt cttttcatgt atgtctcctc tgagtatagt
      481 tctgaacgga tatacatact taatgaaagt atgaggtaaa cgattgtatc atattatcca
      541 gtctttcttg cgtacgcgct tcggctatta tacgcatgat cggttcggta ttggacttgc
      601 gaaaatgaat ccatccgtct tcgaaaataa ttttaacacc atccaccgta tcaacacgtc
      661 cttttttctg atccttaagt attttggtca tgacagcatc ggcgtcaata tcatgcaatt
      721 cgattttatc ttttttcatg ataaaataag gaagctcggc tacacattct gaaaacggct
      781 tatttttttg taacacatac tgtaaggcaa gagcaatccc ggtacatgca tcgcgcccaa
      841 gatgaatttc cggtaagatc acgcccccgt taccttcgcc gccgataacg gagtctatct
      901 cttccatttt ggtggaaaca ttgatttcac caatgcgcgt atggtgagtt tgaacgccgt
      961 acatggtggc aatttgatct atgcccatcg tggtggatgc attgacgaca accggaccct
     1021 ttttgcgtga taaaacgagg tccgttgcga tcattaaggt caattcttca ccgatggctt
     1081 ggcctttttc gttgaccaat gctaacctat cgacatcagg atcaacggca aaaccaatat
     1141 gggcatgatg ttggcgaacc gtttcttcga gtgcttttag attatcaaca gatggctccg
     1201 gattacgagg aaatacgccg tgtggttctt cattgatcac cactacgtta caccccagtt
     1261 ctttcagaag tctgggtacg atcacgccgc ctgcgccatt cacgcagtcc actacaactt
     1321 t
//
